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Sample records for 6-4 pyrimidone photoproducts

  1. DNA photochemistry: geometrically unconstrained pyrimidine (6-4) pyrimidone photoproducts do photoisomerize.

    PubMed

    Douki, Thierry; Rebelo-Moreira, Silvestre; Hamon, Nadège; Bayle, Pierre-Alain

    2015-01-16

    Structural features are of major importance for the formation of mutagenic photoproducts in DNA. It was recently reported that lack of constraints between two adjacent nucleosidic units prevents the conversion of pyrimidine (6-4) pyrimidone photoproducts into their Dewar valence isomers. We here report that this is not the case for the thymidine photoproducts which, although unconstrained, are quantitatively converted into photolysis products identified as Dewar valence isomers by mass spectrometry and NMR and infrared spectroscopies.

  2. Structural basis of pyrimidine-pyrimidone (6-4) photoproduct recognition by UV-DDB in the nucleosome.

    PubMed

    Osakabe, Akihisa; Tachiwana, Hiroaki; Kagawa, Wataru; Horikoshi, Naoki; Matsumoto, Syota; Hasegawa, Mayu; Matsumoto, Naoyuki; Toga, Tatsuya; Yamamoto, Junpei; Hanaoka, Fumio; Thomä, Nicolas H; Sugasawa, Kaoru; Iwai, Shigenori; Kurumizaka, Hitoshi

    2015-01-01

    UV-DDB, an initiation factor for the nucleotide excision repair pathway, recognizes 6-4PP lesions through a base flipping mechanism. As genomic DNA is almost entirely accommodated within nucleosomes, the flipping of the 6-4PP bases is supposed to be extremely difficult if the lesion occurs in a nucleosome, especially on the strand directly contacting the histone surface. Here we report that UV-DDB binds efficiently to nucleosomal 6-4PPs that are rotationally positioned on the solvent accessible or occluded surface. We determined the crystal structures of nucleosomes containing 6-4PPs in these rotational positions, and found that the 6-4PP DNA regions were flexibly disordered, especially in the strand exposed to the solvent. This characteristic of 6-4PP may facilitate UV-DDB binding to the damaged nucleosome. We present the first atomic-resolution pictures of the detrimental DNA cross-links of neighboring pyrimidine bases within the nucleosome, and provide the mechanistic framework for lesion recognition by UV-DDB in chromatin. PMID:26573481

  3. Role of the carbonyl group of the (6-4) photoproduct in the (6-4) photolyase reaction.

    PubMed

    Yamamoto, Junpei; Hitomi, Kenichi; Hayashi, Ryosuke; Getzoff, Elizabeth D; Iwai, Shigenori

    2009-10-01

    The (6-4) photoproduct, which is one of the major UV-induced DNA lesions, causes carcinogenesis with high frequency. The (6-4) photolyase is a flavoprotein that can restore this lesion to the original bases, but its repair mechanism has not been elucidated. In this study, we focused on the interaction between the enzyme and the 3' pyrimidone component of the (6-4) photoproduct and prepared a substrate analogue in which the carbonyl group, a hydrogen-bond acceptor, was replaced with an imine, a hydrogen-bond donor, to investigate the involvement of this carbonyl group in the (6-4) photolyase reaction. UV irradiation of oligodeoxyribonucleotides containing a single thymine-5-methylisocytosine site yielded products with absorption bands at wavelengths longer than 300 nm, similar to those obtained from the conversion of the TT site to the (6-4) photoproduct. Nuclease digestion, MALDI-TOF mass spectrometry, and the instability of the products indicated the formation of the 2-iminopyrimidine-type photoproduct. Analyses of the reaction and the binding of the (6-4) photolyase using these oligonucleotides revealed that this imine analogue of the (6-4) photoproduct was not repaired by the (6-4) photolyase, although the enzyme bound to the oligonucleotide with considerable affinity. These results indicate that the carbonyl group of the 3' pyrimidone ring plays an important role in the (6-4) photolyase reaction. On the basis of these results, we discuss the repair mechanism. PMID:19715341

  4. DNA sequence context greatly affects the accuracy of bypass across an ultraviolet light 6-4 photoproduct in mammalian cells.

    PubMed

    Shriber, Pola; Leitner-Dagan, Yael; Geacintov, Nicholas; Paz-Elizur, Tamar; Livneh, Zvi

    2015-10-01

    Translesion DNA synthesis (TLS) is a DNA damage tolerance mechanism carried out by low-fidelity DNA polymerases that bypass DNA lesions, which overcomes replication stalling. Despite the miscoding nature of most common DNA lesions, several of them are bypassed in mammalian cells in a relatively accurate manner, which plays a key role maintaining a low mutation load. Whereas it is generally agreed that TLS across the major UV and sunlight induced DNA lesion, the cyclobutane pyrimidine dimer (CPD), is accurate, there were conflicting reports on whether the same is true for the thymine-thymine pyrimidine-pyrimidone(6-4) ultraviolet light photoproduct (TT6-4PP), which represents the second most common class of UV lesions. Using a TLS assay system based on gapped plasmids carrying site-specific TT6-4PP lesions in defined sequence contexts we show that the DNA sequence context markedly affected both the extent and accuracy of TLS. The sequence exhibiting higher TLS exhibited also higher error-frequency, caused primarily by semi-targeted mutations, at the nearest nucleotides flanking the lesion. Our results resolve the discrepancy reported on TLS across TT6-4PP, and suggest that TLS is more accurate in human cells than in mouse cells.

  5. Repair of the (6-4) photoproduct by DNA photolyase requires two photons.

    PubMed

    Yamamoto, Junpei; Martin, Ryan; Iwai, Shigenori; Plaza, Pascal; Brettel, Klaus

    2013-07-15

    It takes two (photons) to tango: Single-turnover flash experiments showed that the flavoenzyme (6-4) photolyase uses a successive two-photon mechanism to repair the UV-induced T(6-4)T lesion in DNA (see picture). The intermediate (X) formed by the first photoreaction is likely to be the oxetane-bridged dimer T(ox)T. The enzyme could stabilize the normally short-lived T(ox)T, allowing repair to be completed by the second photoreaction. PMID:23761226

  6. Fully functional global genome repair of (6-4) photoproducts and compromised transcription-coupled repair of cyclobutane pyrimidine dimers in condensed mitotic chromatin

    SciTech Connect

    Komura, Jun-ichiro; Ikehata, Hironobu; Mori, Toshio; Ono, Tetsuya

    2012-03-10

    During mitosis, chromatin is highly condensed, and activities such as transcription and semiconservative replication do not occur. Consequently, the condensed condition of mitotic chromatin is assumed to inhibit DNA metabolism by impeding the access of DNA-transacting proteins. However, about 40 years ago, several researchers observed unscheduled DNA synthesis in UV-irradiated mitotic chromosomes, suggesting the presence of excision repair. We re-examined this subject by directly measuring the removal of UV-induced DNA lesions by an ELISA and by a Southern-based technique in HeLa cells arrested at mitosis. We observed that the removal of (6-4) photoproducts from the overall genome in mitotic cells was as efficient as in interphase cells. This suggests that global genome repair of (6-4) photoproducts is fully functional during mitosis, and that the DNA in mitotic chromatin is accessible to proteins involved in this mode of DNA repair. Nevertheless, not all modes of DNA repair seem fully functional during mitosis. We also observed that the removal of cyclobutane pyrimidine dimers from the dihydrofolate reductase and c-MYC genes in mitotic cells was very slow. This suggests that transcription-coupled repair of cyclobutane pyrimidine dimers is compromised or non-functional during mitosis, which is probably the consequence of mitotic transcriptional repression. -- Highlights: Black-Right-Pointing-Pointer Global genome repair of (6-4) photoproducts is fully active in mitotic cells. Black-Right-Pointing-Pointer DNA in condensed mitotic chromatin does not seem inaccessible or inert. Black-Right-Pointing-Pointer Mitotic transcriptional repression may impair transcription-coupled repair.

  7. Functional Conversion of CPD and (6-4) Photolyases by Mutation.

    PubMed

    Yamada, Daichi; Dokainish, Hisham M; Iwata, Tatsuya; Yamamoto, Junpei; Ishikawa, Tomoko; Todo, Takeshi; Iwai, Shigenori; Getzoff, Elizabeth D; Kitao, Akio; Kandori, Hideki

    2016-08-01

    Ultraviolet (UV) light from the sun damages DNA by forming a cyclobutane pyrimidine dimer (CPD) and pyrimidine(6-4)pyrimidone photoproducts [(6-4) PP]. Photolyase (PHR) enzymes utilize near-UV/blue light for DNA repair, which is initiated by light-induced electron transfer from the fully reduced flavin adenine dinucleotide chromophore. Despite similar structures and repair mechanisms, the functions of PHR are highly selective; CPD PHR repairs CPD, but not (6-4) PP, and vice versa. In this study, we attempted functional conversion between CPD and (6-4) PHRs. We found that a triple mutant of (6-4) PHR is able to repair the CPD photoproduct, though the repair efficiency is 1 order of magnitude lower than that of wild-type CPD PHR. Difference Fourier transform infrared spectra for repair demonstrate the lack of secondary structural alteration in the mutant, suggesting that the triple mutant gains substrate binding ability while it does not gain the optimized conformational changes from light-induced electron transfer to the release of the repaired DNA. Interestingly, the (6-4) photoproduct is not repaired by the reverse mutation of CPD PHR, and eight additional mutations (total of 11 mutations) introduced into CPD PHR are not sufficient. The observed asymmetric functional conversion is interpreted in terms of a more complex repair mechanism for (6-4) repair, which was supported by quantum chemical/molecular mechanical calculation. These results suggest that CPD PHR may represent an evolutionary origin for photolyase family proteins. PMID:27431478

  8. Structural Changes of the Active Center during the Photoactivation of Xenopus (6-4) Photolyase.

    PubMed

    Yamada, Daichi; Yamamoto, Junpei; Zhang, Yu; Iwata, Tatsuya; Hitomi, Kenichi; Getzoff, Elizabeth D; Iwai, Shigenori; Kandori, Hideki

    2016-02-01

    Photolyases (PHRs) repair the UV-induced photoproducts, cyclobutane pyrimidine dimer (CPD) or pyrimidine-pyrimidone (6-4) photoproduct [(6-4) PP], restoring normal bases to maintain genetic integrity. CPD and (6-4) PP are repaired by substrate-specific PHRs, CPD PHR and (6-4) PHR, respectively. Flavin adenine dinucleotide (FAD) is the chromophore of both PHRs, and the resting oxidized form (FAD(ox)), at least under in vitro purified conditions, is first photoconverted to the neutral semiquinoid radical (FADH(•)) form, followed by photoconversion into the enzymatically active fully reduced (FADH(-)) form. Previously, we reported light-induced difference Fourier transform infrared (FTIR) spectra corresponding to the photoactivation process of Xenopus (6-4) PHR. Spectral differences between the absence and presence of (6-4) PP were observed in the photoactivation process. To identify the FTIR signals where these differences appeared, we compared the FTIR spectra of photoactivation (i) in the presence and absence of (6-4) PP, (ii) of (13)C labeling, (15)N labeling, and [(14)N]His/(15)N labeling, and (iii) of H354A and H358A mutants. We successfully assigned the vibrational bands for (6-4) PP, the α-helix and neutral His residue(s). In particular, we assigned three bands to the C ═ O groups of (6-4) PP in the three different redox states of FAD. Furthermore, the changed hydrogen bonding environments of C ═ O groups of (6-4) PP suggested restructuring of the binding pocket of the DNA lesion in the process of photoactivation. PMID:26719910

  9. Induction of (6-4) photolyase gene transcription by blue light in Xenopus A6 cells.

    PubMed

    Fukushima, Noriko; Naito, Yutaka; Ryoji, Masaru

    2009-05-29

    (6-4) photolyase repairs pyrimidine-pyrimidone (6-4) photoproducts generated in DNA upon UV light exposure. We studied the effects of blue light on the expression of this gene in Xenopus A6 cells. Exposure of the cells to blue light, but not red light, for 12h resulted in more than 20-fold increase of the (6-4) photolyase mRNA. By contrast, levels of the other two photolyase mRNAs, i.e., those for CPD photolyase and cryptochrome DASH, did not change significantly. Oxygen radicals presumably generated within the cells upon exposure to blue light were not the cause of the induction, since addition of neither hydrogen peroxide nor a photosensitizer, phenol red, in the culture medium increased the (6-4) photolyase mRNA level. These results support the possibility that the expression of (6-4) photolyase may be regulated by a mechanism involving an as yet ill-defined blue light photoreceptor in the peripheral tissues of Xenopus. PMID:19345672

  10. Resistance of the genome of Escherichia coli and Listeria monocytogenes to irradiation evaluated by the induction of cyclobutane pyrimidine dimers and 6-4 photoproducts using gamma and UV-C radiations

    NASA Astrophysics Data System (ADS)

    Beauchamp, S.; Lacroix, M.

    2012-08-01

    The effect of gamma and UV-C irradiation on the production of cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4 PPs) in DNA was investigated to compare the natural resistance of the genome of a Gram-positive bacterium and a Gram-negative bacterium against irradiation. Solution of pure DNA and bacterial strains Listeria monocytogenes and Escherichia coli were irradiated using gamma and UV-C rays. Extracted DNA from bacteria and pure DNA samples were then analysed by ELISA using anti-CPDs and anti-6-4 PPs monoclonal antibodies. The results show that gamma rays, as well as UV-C rays, induce the formation of CPDs and 6-4 PPs in DNA. During UV-C irradiation, the three samples showed a difference in their sensitivity against formation of CPDs (P≤0.05). Pure DNA was the most sensitive while the genome of L. monocytogenes was the most resistant. Also during UV-C irradiation, the genome of L. monocytogenes was the only one to show a significant resistance against formation of 6-4 PPs (P≤0.05). During gamma irradiation, for both types of lesion, pure DNA and the genome of E. coli did not show significant difference in their sensitivity (P>0.05) while the genome of L. monocytogenes showed a resistance against formation of CPDs and 6-4 PPs.

  11. Reversible Hydrolysis Reaction with the Spore Photoproduct under Alkaline Conditions.

    PubMed

    Adhikari, Surya; Lin, Gengjie; Li, Lei

    2016-09-16

    DNA lesions may reduce the electron density at the nucleobases, making them prone to further modifications upon the alkaline treatment. The dominant DNA photolesion found in UV-irradiated bacterial endospores is a thymine dimer, 5-thyminyl-5,6-dihydrothymine, i.e., the spore photoproduct (SP). Here we report a stepwise addition/elimination reaction in the SP hydrolysis product under strong basic conditions where a ureido group is added to the carboxyl moiety to form a cyclic amide, regenerating SP after eliminating a hydroxide ion. Direct amidation of carboxylic acids by reaction with amines in the presence of a catalyst is well documented; however, it is very rare for an amidation reaction to occur without activation. This uncatalyzed SP reverse reaction in aqueous solution is even more surprising because the carboxyl moiety is not a good electrophile due to the negative charge it carries. Examination of the base-catalyzed hydrolyses of two other saturated pyrimidine lesions, 5,6-dihydro-2'-deoxyuridine and pyrimidine (6-4) pyrimidone photoproduct, reveals that neither reaction is reversible even though all three hydrolysis reactions may share the same gem-diol intermediate. Therefore, the SP structure where the two thymine residues maintain a stacked conformation likely provides the needed framework enabling this highly unusual carboxyl addition/elimination reaction. PMID:27537985

  12. Different removal of ultraviolet photoproducts in genetically related xeroderma pigmentosum and trichothiodystrophy diseases.

    PubMed

    Eveno, E; Bourre, F; Quilliet, X; Chevallier-Lagente, O; Roza, L; Eker, A P; Kleijer, W J; Nikaido, O; Stefanini, M; Hoeijmakers, J H

    1995-10-01

    To understand the heterogeneity in genetic predisposition to skin cancer in different nucleotide excision repair-deficient human syndromes, we studied repair of cyclobutane pyrimidine dimers (CPDs) and of pyrimidine(6-4)pyrimidone (6-4PP) photoproducts in cells from trichothiodystrophy (TTD) patients. TTD is not associated with increased incidence of skin cancer, although 50% of the patients are photosensitive and carry a defect in the nucleotide excision repair pathway, similar to Xeroderma pigmentosum patients. However, in striking contrast to TTD, Xeroderma pigmentosum is highly prone to cancer. To address this apparent paradox, two types of studies were conducted: (a) reactivation of UV-irradiated plasmids harboring actively transcribed reporter genes, with or without photolyase treatment before transfection of SV40-transformed fibroblasts; and (b) the kinetics of removal of UV-induced CPDs and 6-4PPs in genomic DNA by immunoblot analysis using lesion-specific mAbs in SV40-transformed and untransformed fibroblasts representative of all genetic TTD complementation groups. Results showed that all cell lines from photosensitive TTD patients efficiently express Cat or luciferase genes in transfected plasmids carrying non-CPD lesions, including 6-4PP, and display wild-type or near-wild-type (50-70% in 3 cell lines) 6-4PP repair in the overall genome after immunoblot analysis. However, CPD lesions (the repair of which is defective in the overall genome) also block the expression of the reporter gene in transfected plasmids. Two cell lines from nonphotosensitive TTD patients showed wild-type levels of repair for both photoproducts in overall genome. A model on the lesion-specific repair in the context of the molecular defect in TTD is proposed. The implication of the defective CPD repair and efficient 6-4PP repair subpathways in cancer prevention in TTD patients is discussed. PMID:7671243

  13. Combined QM/MM investigation on the light-driven electron-induced repair of the (6-4) thymine dimer catalyzed by DNA photolyase.

    PubMed

    Faraji, Shirin; Groenhof, Gerrit; Dreuw, Andreas

    2013-09-01

    The (6-4) photolyases are blue-light-activated enzymes that selectively bind to DNA and initiate splitting of mutagenic thymine (6-4) thymine photoproducts (T(6-4)T-PP) via photoinduced electron transfer from flavin adenine dinucleotide anion (FADH(-)) to the lesion triggering repair. In the present work, the repair mechanism after the initial electron transfer and the effect of the protein/DNA environment are investigated theoretically by means of hybrid quantum mechanical/molecular mechanical (QM/MM) simulations using X-ray structure of the enzyme-DNA complex. By comparison of three previously proposed repair mechanisms, we found that the lowest activation free energy is required for the pathway in which the key step governing the repair photocycle is electron transfer coupled with the proton transfer from the protonated histidine, His365, to the N3' nitrogen of the pyrimidone thymine. The transfer simultaneously occurs with concerted intramolecular OH transfer without formation of an oxetane or isolated water molecule intermediate. In contrast to previously suggested mechanisms, this newly identified pathway requires neither a subsequent two-photon process nor electronic excitation of the photolesion.

  14. 2-Ureido-4-pyrimidone-based hydrogels with multiple responses.

    PubMed

    Cui, Jiaxi; Wang, Dapeng; Koynov, Kaloian; del Campo, Aránzazu

    2013-09-16

    Functionalisation of poly[2-(dimethylamino)ethyl methacrylate] (a responsive methacrylate) with light-activatable 2-ureido-4-pyrimidone units allows a supramolecular hydrogel to be obtained that combines temperature, light and pH response with self-healing properties. Whereas the self-healing properties of this system were described previously, this report focuses on its response to different external stimuli, which is studied by quartz crystal microbalance analysis of thin films of the material. Reversible collapse with increasing temperature, reversible swelling with decreasing pH and irreversible shrinkage with UV exposure are demonstrated. These three stimuli are combined to have externally gated or tuned responses. Thermo-induced swelling and shrinkage can be reversibly inhibited by changing the pH and irreversibly regulated by exposure to light of different doses. These materials represent the first general strategy to obtain responsive self-healing hydrogels in which the response and the self-healing properties are decoupled from each other and can be tuned independently. PMID:23918634

  15. Pyrimidone-based series of glucokinase activators with alternative donor-acceptor motif.

    PubMed

    Filipski, Kevin J; Guzman-Perez, Angel; Bian, Jianwei; Perreault, Christian; Aspnes, Gary E; Didiuk, Mary T; Dow, Robert L; Hank, Richard F; Jones, Christopher S; Maguire, Robert J; Tu, Meihua; Zeng, Dongxiang; Liu, Shenping; Knafels, John D; Litchfield, John; Atkinson, Karen; Derksen, David R; Bourbonais, Francis; Gajiwala, Ketan S; Hickey, Michael; Johnson, Theodore O; Humphries, Paul S; Pfefferkorn, Jeffrey A

    2013-08-15

    Glucokinase activators are a class of experimental agents under investigation as a therapy for Type 2 diabetes mellitus. An X-ray crystal structure of a modestly potent agent revealed the potential to substitute the common heterocyclic amide donor-acceptor motif for a pyridone moiety. We have successfully demonstrated that both pyridone and pyrimidone heterocycles can be used as a potent donor-acceptor substituent. Several sub-micromolar analogs that possess the desired partial activator profile were synthesized and characterized. Unfortunately, the most potent activators suffered from sub-optimal pharmacokinetic properties. Nonetheless, these donor-acceptor motifs may find utility in other glucokinase activator series or beyond.

  16. HTS followed by NMR based counterscreening. Discovery and optimization of pyrimidones as reversible and competitive inhibitors of xanthine oxidase.

    PubMed

    Evenäs, Johan; Edfeldt, Fredrik; Lepistö, Matti; Svitacheva, Naila; Synnergren, Anna; Lundquist, Britta; Gränse, Mia; Rönnholm, Anna; Varga, Mikael; Wright, John; Wei, Min; Yue, Sherrie; Wang, Junfeng; Li, Chong; Li, Xuan; Chen, Gang; Liao, Yong; Lv, Gang; Tjörnebo, Ann; Narjes, Frank

    2014-03-01

    The identification of novel, non-purine based inhibitors of xanthine oxidase is described. After a high-throughput screening campaign, an NMR based counterscreen was used to distinguish actives, which interact with XO in a reversible manner, from assay artefacts. This approach identified pyrimidone 1 as a reversible and competitive inhibitor with good lead-like properties. A hit to lead campaign gave compound 41, a nanomolar inhibitor of hXO with efficacy in the hyperuricemic rat model after oral dosing.

  17. Quantum chemical study of the enzymatic repair of T(6-4)C/C(6-4)T UV-photolesions by DNA photolyases.

    PubMed

    Faraji, Shirin; Wirz, Lukas; Dreuw, Andreas

    2013-08-26

    Several strategies have evolved to repair one of the abundant UV radiation-induced damages caused to DNA, namely the mutagenic pyrimidine (6-4) pyrimidone photolesions. DNA (6-4)-photolyases are enzymes repairing these lesions by a photoinitiated electron transfer. An important aspect of a possible repair mechanism is its generality and transferability to different (6-4) lesions. Therefore, previously suggested mechanisms for the repair of the T(6-4)T lesion are here transferred to the T(6-4)C and C(6-4)T lesions and investigated theoretically using quantum chemical methods. Despite the different functional groups of the pyrimidine bases involved, a general valid molecular mechanism was identified, in which the initial step is an electron transfer coupled to a proton transfer from the protonated HIS365 to the N3(') nitrogen of the 3(') pyrimidine, followed by an intramolecular OH/NH2 transfer in one concerted step, which does not require an oxetane/azetidine or isolated water/ammonia intermediate.

  18. Meson photoproduction (CLAS)

    SciTech Connect

    Steffen Strauch

    2009-10-01

    This is a brief and selective discussion of meson photoproduction measurements with the CEBAF Large Acceptance Spectrometer (CLAS) at Jefferson Lab. Meson photo- production is being used as a tool for various investigations, including the spectroscopy of baryons and mesons and the search for vector-meson medium modifications.

  19. Backward pion photoproduction

    SciTech Connect

    Sibirtsev, A; Haidenbauer, J; Huang, F; Krewald, S; Meissner, U -G

    2009-04-01

    We present a systematic analysis of backward pion photoproduction for the reactions $ \\gamma$ p $ \\rightarrow$ $ \\pi^{0}_{}$ p and $ \\gamma$ p $ \\rightarrow$ $ \\pi^{+}_{}$ n . Regge phenomenology is applied at invariant collision energies above 3GeV in order to fix the reaction amplitude. A comparision with older data on $ \\pi^{0}_{}$ - and $ \\pi^{+}_{}$ -photoproduction at $ \\vartheta$ = 180° indicates that the high-energy limit as given by the Regge calculation could be reached possibly at energies of around $ \\sqrt{{s}}$ ≃ 3 GeV. In the energy region of $ \\sqrt{{s}}$ $ \\le$2.5 GeV, covered by the new measurements of $ \\gamma$ p $ \\rightarrow$ $ \\pi^{0}_{}$ p differential cross-sections at large angles at ELSA, JLab, and LEPS, we see no clear signal for a convergence towards the Regge results. The baryon trajectories obtained in our analysis are in good agreement with those given by the spectrum of excited baryons.

  20. Extracting amplitudes from photoproduction data

    NASA Astrophysics Data System (ADS)

    Workman, R. L.

    2011-09-01

    We consider the problems associated with amplitude extraction, from meson photoproduction data, over the first resonance regions. The notion of a complete experiment has motivated the FROST program at Jefferson Lab. Exercises applied to pion photoproduction data illustrate the problems to be confronted in any attempt to extract underlying resonance signals from these data (without introducing a model for the resonant process).

  1. Fluoroimaging-based immunoassay of DNA photoproducts in ultraviolet-B-irradiated tadpoles.

    PubMed

    Pandelova, Iovanna; Hewitt, Stephen R; Hays, John B

    2005-01-01

    Sensitive and accurate measurement of photoproducts induced in DNA by natural or artificial ultraviolet-B (UVB; and UVC) light is essential to evaluate the toxic and mutagenic effects of this radiation. Monoclonal antibodies specific for the two major classes of photoproducts-cyclobutane pyrimidine dimers (CPDs) and pyrimidine-[6-4]-pyrimidinone photoproducts ([6-4]PPs)-have made possible highly specific and sensitive assays. Described here is the use of these primary antibodies with fluorescent secondary antibodies to generate 96-spot arrays. Stable fluorescence signals are rapidly and sensitively scored by fluoroimaging and computer analysis of peak-and-valley traces. CPD levels in a series of calibration standards are determined by acid hydrolysis/thin-layer chromatography analyses of radiolabeled bacterial DNA, UV-irradiated to known high fluences, and linear extrapolation to known lower fluences. The nonlinear fluorescence vs CPD curve reflects the effect of photoproduct concentration on single vs double binding by divalent antibody proteins. This technique is applied to photoproducts in whole inbred Xenopus laevis tadpoles, chronically irradiated at a series of UVB fluences that reach a lethality threshold when in vivo steady-state photoproduct levels are still quite low. As few as 0.01-0.02 CPDs per DNA kbp can be reliably detected, at signal/noise ratios of roughly 3:1. PMID:15502209

  2. FTIR study of light-dependent activation and DNA repair processes of (6-4) photolyase.

    PubMed

    Zhang, Yu; Iwata, Tatsuya; Yamamoto, Junpei; Hitomi, Kenichi; Iwai, Shigenori; Todo, Takeshi; Getzoff, Elizabeth D; Kandori, Hideki

    2011-05-10

    The UV component of sunlight threatens all life on the earth by damaging DNA. The photolyase (PHR) DNA repair proteins maintain genetic integrity by harnessing blue light to restore intact bases from the major UV-induced photoproducts, cyclobutane pyrimidine dimers (CPD), and (6-4) photoproducts ((6-4) PPs). The (6-4) PHR must catalyze not only covalent bond cleavage between two pyrmidine bases but also hydroxyl or amino group transfer from the 5'- to 3'-pyrimidine base, requiring a more complex mechanism than that postulated for CPD PHR. In this paper, we apply Fourier transform infrared (FTIR) spectroscopy to (6-4) PHR and report difference FTIR spectra that correspond to its photoactivation, substrate binding, and light-dependent DNA repair processes. The presence of DNA carrying a single (6-4) PP uniquely influences vibrations of the protein backbone and a protonated carboxylic acid, whereas photoactivation produces IR spectral changes for the FAD cofactor and the surrounding protein. Difference FTIR spectra for the light-dependent DNA damage repair reaction directly show significant DNA structural changes in the (6-4) lesion and the neighboring phosphate group. Time-dependent illumination of samples with different enzyme:substrate stoichiometries successfully distinguished signals characteristic of structural changes in the protein and the DNA resulting from binding and catalysis. PMID:21462921

  3. Photoproduction of hybrid mesons

    SciTech Connect

    Barnes, T. |

    1998-11-01

    In this contribution the author discusses prospects for photoproducing hybrid mesons at CEBAF, based on recent model results and experimental indications of possible hybrids. One excellent opportunity appears to be a search for I = 1, J{sup PC} = 2{sup +{minus}} ``b{sub 2}{sup o}`` hybrids in (a{sub 2}{pi}){sup o} through diffraction photoproduction. Other notable possibilities accessible through {pi}{sup +}; {pi}{sub J}{sup +}(1770) in f{sub 2}{pi}{sup +} and (b{sub 1}{pi}){sup +}; {pi}{sup +}(1800) in f{sub 0}{pi}{sup +}, f{sub 2}{pi}{sup =}, {rho}{sup +}{omega} and ({rho}{pi}){sup +}; a{sub 1} in f{sub 1}{pi}{sup +} and f{sub 2}{pi}{sup +}; and {omega} in ({rho}{pi}){sup o}, {omega}{eta} and K{sub 1}K.

  4. phi Photoproduction at CLAS

    SciTech Connect

    E. Anciant; G. Audit; T. Auger; M. Battaglieri; J.M. Laget; C. Marchand; K. McCormick; CLAS Collaboration

    2000-05-12

    The cross-section for phi meson photoproduction on the proton has been measured at a photon energy E_gamma = 3.6 GeV up to a four-momentum transfer -t = 4 GeV^2. A measurement of the differential cross-section at such high transfers, down to 100 pb/GeV^2, was only made possible thanks to the combination of the 100% duty cycle of CEBAF and the use of the large acceptance detector CLAS. At low four-momentum transfer, the differential cross-section is in agreement with previous measurements and is well accounted for by the exchange of the Pomeron. At large four-momentum transfer, above -t = 1 GeV^2, no previous data exist, and our results support a model where the Pomeron is resolved into its simplest component, two gluons. Taking into account the coupling of these gluons to any quark in the proton and the phi reproduces quite well the dependence in t of our cross-sections.

  5. Biological hydrogen photoproduction

    SciTech Connect

    Nemoto, Y.

    1995-09-01

    Following are the major accomplishments of the 6th year`s study of biological hydrogen photoproduction which were supported by DOE/NREL. (1) We have been characterizing a biological hydrogen production system using synchronously growing aerobically nitrogen-fixing unicellular cyanobacterium, Synechococcus sp. Miami BG 043511. So far it was necessary to irradiate the cells to produce hydrogen. Under darkness they did not produce hydrogen. However, we found that, if the cells are incubated with oxygen, they produce hydrogen under the dark. Under 80% argon + 20% oxygen condition, the hydrogen production activity under the dark was about one third of that under the light + argon condition. (2) Also it was necessary so far to incubate the cells under argon atmosphere to produce hydrogen in this system. Argon treatment is very expensive and should be avoided in an actual hydrogen production system. We found that, if the cells are incubated at a high cell density and in a container with minimum headspace, it is not necessary to use argon for the hydrogen production. (3) Calcium ion was found to play an important role in the mechanisms of protection of nitrogenase from external oxygen. This will be a clue to understand the reason why the hydrogen production is so resistant to oxygen in this strain. (4) In this strain, sulfide can be used as electron donor for the hydrogen production. This result shows that waste water can be used for the hydrogen production system using this strain.

  6. Bound transcription factor suppresses photoproduct formation in the NF-kappa B promoter.

    PubMed

    Ghosh, R; Paniker, L; Mitchell, D L

    2001-01-01

    The relationship between purified transcription factor p50 binding and ultraviolet light-induced DNA damage formation in the NF-kappa B promoter element was investigated. The effect of bound transcription factor on cyclobutane dimer formation was quantified using Maxam-Gilbert analysis of irradiated substrate digested with T4 phage endonuclease V. Two methods were employed for cleaving (6-4) photoproducts. Sites of (6-4) photoproducts cleaved by piperidine showed a general suppression in the presence of bound p50 protein similar to that observed for cyclobutane dimers. In contrast to piperidine, digestion with ultraviolet damage endonuclease (UVDE) from Saccharomyces pombe subsequent to cyclobutane dimer reversal by photolyase displayed a broader spectrum of damaged sites. Whereas some of these sites were suppressed by bound p50 protein, some remained unaffected and one site showed increased (6-4) photoproduct induction. These data illustrate the advantage of UVDE over piperidine for studying (6-4) photoproducts at the sequence level and suggest that this approach may be useful for footprinting transcription factor binding in other promoters.

  7. Induction of UV photoproducts and DNA damage by solar simulator UV irradiation

    SciTech Connect

    Wolfreys, A.; Henderson, L.; Clingen, P.

    1997-10-01

    The recent increased incidence of skin cancer and the depletion of the ozone layer has increased interest in the ultraviolet (UV) component of natural sunlight and its role in the induction of skin cancer. Previous research on UV radiation has concentrated on UVC (254nm) but, as only UVB and UVA are present in natural sunlight, its relevance is unknown. We have investigated the induction of two forms of direct DNA damage - the pyrimidine dimer and the (6-4) photoproduct - in human DNA repair deficient XP-G (Xeroderma pigmentosum group G) lymphoblastoid cells following exposure to simulated sunlight. As exposure to natural sunlight is highly variable, a solar simulator lamp was used which is known to mimic natural sunlight at midday in Central Europe. Cells were irradiated on ice to minimise DNA repair and the relative induction of pyrimidine dimers and (6-4) photoproducts was measured using specific monoclonal antibodies and a computer assisted image analysis system. A time dependent increase in both cyclobutane dimer and (6-4) photoproduct antibody binding sites was seen. The increases in pyrimidine dimer and (6-4) photoproduct antibody binding sites differed to that reported with natural sunlight in the UK but was similar to that seen with a similar solar simulator lamp.

  8. Charmed hadron photoproduction at COMPASS

    NASA Astrophysics Data System (ADS)

    Wang, Xiao-Yun; Guskov, Alexey

    2016-06-01

    Photoproduction of the charmonium-like state Zc(4200) and the charmed baryon Λ_c^* (2940) is investigated with an effective Lagrangian approach and the Regge trajectories applying to the COMPASS experiment. Combining the experimental data from COMPASS and our theoretical model we estimate the upper limit of ΓZc(4200)→J/ψπ to be of about 37 MeV. Moreover, the possibility to produce Λ_c^* (2940) at COMPASS is discussed. It seems one can try to search for this hadron in the missing mass spectrum since the t-channel is dominating for the Λ_c^* (2940) photoproduction.

  9. Heavy Quark Photoproduction at LHC

    SciTech Connect

    Goncalves, V. P.; Meneses, A. R.; Machado, M. V.

    2010-11-12

    In this work we calculate the inclusive and difractive photoproduction of heavy quarks in proton-proton collisions at LHC energies within the color dipole picture employing three phenomenological saturation models based on the color glass condensate formalism. Our results demonstrate that the experimental analyzes of these reactions is feasible and that the cross sections are sensitive to the underlying parton dynamics.

  10. 27 CFR 6.4 - Jurisdictional limits.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Jurisdictional limits. 6.4 Section 6.4 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS âTIED-HOUSEâ Scope of Regulations § 6.4 Jurisdictional limits. (a) General. The regulations in this part apply where:...

  11. 27 CFR 6.4 - Jurisdictional limits.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false Jurisdictional limits. 6.4 Section 6.4 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS âTIED-HOUSEâ Scope of Regulations § 6.4 Jurisdictional limits. (a) General. The regulations in this part apply where:...

  12. 27 CFR 6.4 - Jurisdictional limits.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Jurisdictional limits. 6.4 Section 6.4 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL âTIED-HOUSEâ Scope of Regulations § 6.4 Jurisdictional limits. (a) General. The regulations in this part apply where:...

  13. 27 CFR 6.4 - Jurisdictional limits.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Jurisdictional limits. 6.4 Section 6.4 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS âTIED-HOUSEâ Scope of Regulations § 6.4 Jurisdictional limits. (a) General. The regulations in this part apply where:...

  14. 27 CFR 6.4 - Jurisdictional limits.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false Jurisdictional limits. 6.4 Section 6.4 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL âTIED-HOUSEâ Scope of Regulations § 6.4 Jurisdictional limits. (a) General. The regulations in this part apply where:...

  15. 42 CFR 6.4 - Covered individuals.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Covered individuals. 6.4 Section 6.4 Public Health... COVERAGE OF CERTAIN GRANTEES AND INDIVIDUALS § 6.4 Covered individuals. (a) Officers and employees of a... if they meet the requirements of section 224(g)(5) of the Act. (c) An individual physician or...

  16. Photoproduction of dissolved inorganic carbon in Swedish lakes

    NASA Astrophysics Data System (ADS)

    Koehler, B.; Landelius, T.; Tranvik, L. J.

    2012-04-01

    A substantial fraction of the dissolved organic carbon (DOC) in inland waters is mineralized to dissolved inorganic carbon (DIC) during passage towards the sea. Both microbial and photochemical mineralization have a share but there is currently no landscape-scale estimate of the contribution of photomineralization to total lake carbon dioxide emissions, restricting our understanding of inland-water C cycling. We use 1) DOC absorbance spectra measured during autumn 2009 in water samples from 1074 lakes distributed across Sweden, 2) light attenuation coefficients estimated based on correlations with absorption coefficients as established from literature data, 3) cloud-corrected, below-water-surface downwelling scalar irradiance spectra derived by modeling radiative transfer in the atmosphere and transmission into the water and 4) an apparent quantum yield spectrum determined in a humic lake, to calculate spectra of DIC photoproduction from 280 to 600 nm and from the water surface down to the mean lake depths. For each lake, we calculate DIC photoproduction rates on a daily base and integrate to obtain yearly flux estimates. Preliminary model results calculated for July 2009 show that, even though water color differed largely (25%- and 75%-quantiles of specific UV absorption coefficients at 254 nm (SUV A254) of 6.4 and 9.6 L mg C-1 m-1, respectively), depth-integrated DIC photoproduction rates showed a relatively small variation with a 25%-quantile of 12.0 and a 75%-quantile of 13.1 mg C m-2 day-1. These rather similar DIC photoproduction rates are explained by their different depth distributions: The brownest lake with a SUV A254 of 12.9 L mg C-1 m-1 had large surface DIC photoproduction rates of 887.9 mg C m-3 day-1 but photons were quickly attenuated with depth, with DIC photoproduction rates falling below 1 mg C m-3 day-1 already at ¯ 0.2 m depth (depth-integrated rate of 14.2 mg C m-2 day-1). The clearest lake with a SUV A254 of 1.4 L mg C-1 m-1 had nearly 100

  17. Study of charm photoproduction mechanisms

    NASA Astrophysics Data System (ADS)

    Alvarez, M. P.; Barate, R.; Bloch, D.; Bonamy, P.; Borgeaud, P.; Burchell, M.; Burmeister, H.; Brunet, J. M.; Calvino, F.; Cattaneo, M.; Crespo, J. M.; D'Almagne, B.; David, M.; di Ciaccio, L.; Druet, P.; Duane, A.; Engel, J. P.; Ferrer, A.; Filippas, T. A.; Fokitis, E.; Forty, R. W.; Foucault, P.; Gazis, E. N.; Gerber, J. P.; Giomataris, Y.; Hall, G.; Hofmokl, T.; Katsoufis, E. C.; Koratzinos, M.; Krafft, C.; Lefievre, B.; Lemoigne, Y.; Lopez, A.; Lui, W. K.; Magneville, C.; Maltezos, A.; McEwen, J. G.; Papadopoulou, Th.; Pattison, B.; Poutot, D.; Primout, M.; Rahmani, H.; Roudeau, P.; Seez, C.; Six, J.; Strub, R.; Treille, D.; Triscos, P.; Tristram, G.; Villet, G.; Volte, A.; Wayne, M.; Websdale, D. M.; Wormser, G.; Zolnierowski, Y.

    1993-03-01

    This paper presents results on charm photoproduction in the energy interval 40 to 160 GeV, obtained from the high-statistics charm samples of the NA 14/2 experiment at CERN. We measure the charm cross-section, the distributions in x F and p {2/ T } and various production ratios and charge asymmetries. The total non-diffractive open-charm cross-section per nucleon is measured to beσ _{(γ N to cbar cX)} at < E γ> =100 GeV. We discuss the photoproduction of charm in terms of theoretical and phenomenological models. We compare the measured p {2/ T } and x F distributions with first-order QCD calculations of photon-gluon fusion and obtain a value for the charm-quark mass of m c =1.5{+0.2/-0.1}GeV/c2.

  18. Photoproduction of exotic baryon resonances

    NASA Astrophysics Data System (ADS)

    Karliner, Marek; Rosner, Jonathan L.

    2016-01-01

    We point out that the new exotic resonances recently reported by LHCb in the J / ψ p channel are excellent candidates for photoproduction off a proton target. This test is crucial to confirming the resonant nature of such states, as opposed to their being kinematical effects. We specialize to an interpretation of the heavier narrow state as a molecule composed of Σc and Dbar*, and estimate its production cross section using vector dominance. The relevant photon energies and fluxes are well within the capabilities of the GlueX and CLAS12 detectors at Thomas Jefferson National Accelerator Facility (JLAB). A corresponding calculation is also performed for photoproduction of an analogous resonance which is predicted to exist in the ϒp channel.

  19. Nuclear shadowing and ρ photoproduction

    NASA Astrophysics Data System (ADS)

    Pautz, A.; Shaw, G.

    1998-05-01

    ρ photoproduction on complex nuclei is reexamined using a generalized vector dominance model which succesfully predicts the oberved nuclear shadowing in real photoabsorption and deep inelastic scattering. This model is shown to give a good fit to ρ photoproduction data on both nucleons and complex nuclei, in which the disagreement between the measured γ-ρ coupling and the γ-ρ coupling required by the simple vector dominance model is eliminated. The ρN total cross sections required are similar to those predicted by the additive quark model, and the magnitude of the correction to simple vector dominance is consistent with that inferred from the analysis of real photoabsorption and deep inelastic scattering.

  20. Meson Photoproduction Experiments with CLAS

    SciTech Connect

    Eugene Pasyuk

    2012-12-01

    A large part of the experimental program in Hall B of the Jefferson Lab is dedicated to light baryon spectroscopy. Meson photoprodcution experiments are essential part of this program. CEBAF Large Acceptance Spectrometer (CLAS) and availability of circularly and linearly polarized tagged photon beams and frozen spin polarized targets provide unique conditions for this type of experiments. This combination of experimental tools gives a remarkable opportunity to measure double polarization observables for different pseudo-scalar meson photoproduction processes. For the first time, a complete or nearly complete measurement became possible and will facilitate model independent extraction of the reaction amplitude. An overview of the experimental program and its current status together with recent results on double polarization measurements in π{sup +} photoproduction are presented.

  1. Bayesian analysis for kaon photoproduction

    SciTech Connect

    Marsainy, T. Mart, T.

    2014-09-25

    We have investigated contribution of the nucleon resonances in the kaon photoproduction process by using an established statistical decision making method, i.e. the Bayesian method. This method does not only evaluate the model over its entire parameter space, but also takes the prior information and experimental data into account. The result indicates that certain resonances have larger probabilities to contribute to the process.

  2. Structural role of two histidines in the (6-4) photolyase reaction

    PubMed Central

    Yamada, Daichi; Iwata, Tatsuya; Yamamoto, Junpei; Hitomi, Kenichi; Todo, Takeshi; Iwai, Shigenori; Getzoff, Elizabeth D.; Kandori, Hideki

    2015-01-01

    Photolyases (PHRs) are DNA repair enzymes that revert UV-induced photoproducts, either cyclobutane pyrimidine dimers (CPD) or (6-4) photoproducts (PPs), into normal bases to maintain genetic integrity. (6-4) PHR must catalyze not only covalent bond cleavage, but also hydroxyl or amino group transfer, yielding a more complex mechanism than that postulated for CPD PHR. Previous mutation analysis revealed the importance of two histidines in the active center, H354 and H358 for Xenopus (6-4) PHR, whose mutations significantly lowered the enzymatic activity. Based upon highly sensitive FTIR analysis of the repair function, here we report that both H354A and H358A mutants of Xenopus (6-4) PHR still maintain their repair activity, although the efficiency is much lower than that of the wild type. Similar difference FTIR spectra between the wild type and mutant proteins suggest a common mechanism of repair in which (6-4) PP binds to the active center of each mutant, and is released after repair, as occurs in the wild type. Similar FTIR spectra also suggest that a decrease in volume by the H-to-A mutation is possibly compensated by the addition of water molecule( s). Such a modified environment is sufficient for the repair function that is probably controlled by proton-coupled electron transfer between the enzyme and substrate. On the other hand, two histidines must work in a concerted manner in the active center of the wild-type enzyme, which significantly raises the repair efficiency. PMID:27493863

  3. 43 CFR 3141.6-4 - Qualifications.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) LEASING IN SPECIAL TAR SAND AREAS Leasing in Special Tar Sand Areas § 3141.6-4 Qualifications. Each bidder shall submit with the bid a statement over...

  4. 43 CFR 3141.6-4 - Qualifications.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) LEASING IN SPECIAL TAR SAND AREAS Leasing in Special Tar Sand Areas § 3141.6-4 Qualifications. Each bidder shall submit with the bid a statement over...

  5. 43 CFR 3141.6-4 - Qualifications.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) LEASING IN SPECIAL TAR SAND AREAS Leasing in Special Tar Sand Areas § 3141.6-4 Qualifications. Each bidder shall submit with the bid a statement over...

  6. 43 CFR 3141.6-4 - Qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) LEASING IN SPECIAL TAR SAND AREAS Leasing in Special Tar Sand Areas § 3141.6-4 Qualifications. Each bidder shall submit with the bid a statement over...

  7. Complete Photo-production Experiments

    SciTech Connect

    D'Angelo, A.; Bartalini, O.; Fantini, A.; Schaerf, C.; Vegna, V.; Ardashev, K.; Bade, C.; Hicks, K.; Kizilgul, S.; Lucas, M.; Mahon, J.; Bellini, V.; Blecher, M.; Bocquet, J.-P.; Lleres, A.; Rebreyend, D.; Capogni, M.; Caracappa, A.; Kistner, O. C.; Miceli, L.

    2011-10-24

    The extraction of resonance parameters from meson photo-reaction data is a challenging effort, that would greatly benefit from the availability of several polarization observables, measured for each reaction channel on both proton and neutron targets. In the aim of obtaining such complete experiments, polarized photon beams and targets have been developed at facilities, worldwide. We report on the latest results from the LEGS and GRAAL collaborations, providing single and double polarization measurements on pseudo-scalar meson photo-production from the nucleon.

  8. Meson Photoproduction Experiments at ELPH, Tohoku University

    NASA Astrophysics Data System (ADS)

    Ishikawa, Takatsugu; Fujimura, Hisako; Fukasawa, Hiroshi; Hashimoto, Ryo; He, Qinghua; Honda, Yuki; Iwata, Takahiro; Kaida, Shun; Kasagi, Jirohta; Kawano, Atsushi; Kuwasaki, Shuzo; Maeda, Kazushige; Masumoto, Shin'ichi; Miyabe, Manabu; Miyahara, Fusashi; Mochizuki, Kei'ichi; Muramatsu, Norihito; Nakamura, Akihiko; Nawa, Ken'ichi; Ogushi, Shoei; Okada, Yasuyuki; Onodera, Yoshihito; Ozawa, Kyoichiro; Sakamoto, Yasunobu; Sato, Mamoru; Shimizu, Hajime; Sugai, Hiroyuki; Suzuki, Koutaku; Tajima, Yasuhisa; Takahashi, Shin'ichiro; Taniguchi, Yusuke; Tsuchikawa, Yusuke; Yamazaki, Hirohito; Yamazaki, Ryuji; Yoshida, Hiroshi Y.

    Meson photoproduction experiments have been conducted with an electromagnetic (EM) calorimeter FOREST at Research Center for Electron Photon Science (ELPH), Tohoku University. A narrow resonance observed at W = 1670 MeV in η photoproduction on the neutron is of great interest, which is a candidate of an anti-decuplet pentaquark baryon although its origin is still controversial. The preliminary results of the cross sections for π0 and η photoproduction on the deuteron are presented. The next generation FOREST experiments have been planned to study S11(1535) properties in the nuclear medium by searching for η-mesic nucleus states. The planned experiments are also shown in this contribution.

  9. Prediction of p38 map kinase inhibitory activity of 3, 4-dihydropyrido [3, 2-d] pyrimidone derivatives using an expert system based on principal component analysis and least square support vector machine.

    PubMed

    Shahlaei, M; Saghaie, L

    2014-01-01

    A quantitative structure-activity relationship (QSAR) study is suggested for the prediction of biological activity (pIC50) of 3, 4-dihydropyrido [3,2-d] pyrimidone derivatives as p38 inhibitors. Modeling of the biological activities of compounds of interest as a function of molecular structures was established by means of principal component analysis (PCA) and least square support vector machine (LS-SVM) methods. The results showed that the pIC50 values calculated by LS-SVM are in good agreement with the experimental data, and the performance of the LS-SVM regression model is superior to the PCA-based model. The developed LS-SVM model was applied for the prediction of the biological activities of pyrimidone derivatives, which were not in the modeling procedure. The resulted model showed high prediction ability with root mean square error of prediction of 0.460 for LS-SVM. The study provided a novel and effective approach for predicting biological activities of 3, 4-dihydropyrido [3,2-d] pyrimidone derivatives as p38 inhibitors and disclosed that LS-SVM can be used as a powerful chemometrics tool for QSAR studies.

  10. Prediction of p38 map kinase inhibitory activity of 3, 4-dihydropyrido [3, 2-d] pyrimidone derivatives using an expert system based on principal component analysis and least square support vector machine

    PubMed Central

    Shahlaei, M.; Saghaie, L.

    2014-01-01

    A quantitative structure–activity relationship (QSAR) study is suggested for the prediction of biological activity (pIC50) of 3, 4-dihydropyrido [3,2-d] pyrimidone derivatives as p38 inhibitors. Modeling of the biological activities of compounds of interest as a function of molecular structures was established by means of principal component analysis (PCA) and least square support vector machine (LS-SVM) methods. The results showed that the pIC50 values calculated by LS-SVM are in good agreement with the experimental data, and the performance of the LS-SVM regression model is superior to the PCA-based model. The developed LS-SVM model was applied for the prediction of the biological activities of pyrimidone derivatives, which were not in the modeling procedure. The resulted model showed high prediction ability with root mean square error of prediction of 0.460 for LS-SVM. The study provided a novel and effective approach for predicting biological activities of 3, 4-dihydropyrido [3,2-d] pyrimidone derivatives as p38 inhibitors and disclosed that LS-SVM can be used as a powerful chemometrics tool for QSAR studies. PMID:26339262

  11. Deformable nature of various damaged DNA duplexes estimated by an electrochemical analysis on electrodes.

    PubMed

    Chiba, J; Aoki, S; Yamamoto, J; Iwai, S; Inouye, M

    2014-10-01

    We report bending flexibility of damaged duplexes possessing an apurinic/apyrimidinic (AP) site analogue, a cyclobutane pyrimidine dimer (CPD), and a pyrimidine(6-4)pyrimidone photoproduct (6-4PP). Based on the electrochemical evaluation on electrodes, the duplex flexibilities of the lesions increased in the following order: CPD < AP < 6-4PP. We discussed the possibility that the emerging local flexibility might be a good sign for UV-damaged DNA-binding proteins on duplexes.

  12. Discovery and optimization of new benzimidazole- and benzoxazole-pyrimidone selective PI3Kβ inhibitors for the treatment of phosphatase and TENsin homologue (PTEN)-deficient cancers.

    PubMed

    Certal, Victor; Halley, Frank; Virone-Oddos, Angela; Delorme, Cécile; Karlsson, Andreas; Rak, Alexey; Thompson, Fabienne; Filoche-Rommé, Bruno; El-Ahmad, Youssef; Carry, Jean-Christophe; Abecassis, Pierre-Yves; Lejeune, Pascale; Vincent, Loic; Bonnevaux, Hélène; Nicolas, Jean-Paul; Bertrand, Thomas; Marquette, Jean-Pierre; Michot, Nadine; Benard, Tsiala; Below, Peter; Vade, Isabelle; Chatreaux, Fabienne; Lebourg, Gilles; Pilorge, Fabienne; Angouillant-Boniface, Odile; Louboutin, Audrey; Lengauer, Christoph; Schio, Laurent

    2012-05-24

    Most of the phosphoinositide-3 kinase (PI3K) kinase inhibitors currently in clinical trials for cancer treatment exhibit pan PI3K isoform profiles. Single PI3K isoforms differentially control tumorigenesis, and PI3Kβ has emerged as the isoform involved in the tumorigenicity of PTEN-deficient tumors. Herein we describe the discovery and optimization of a new series of benzimidazole- and benzoxazole-pyrimidones as small molecular mass PI3Kβ-selective inhibitors. Starting with compound 5 obtained from a one-pot reaction via a novel intermediate 1, medicinal chemistry optimization led to the discovery of compound 8, which showed a significant activity and selectivity for PI3Kβ and adequate in vitro pharmacokinetic properties. The X-ray costructure of compound 8 in PI3Kδ showed key interactions and structural features supporting the observed PI3Kβ isoform selectivity. Compound 8 achieved sustained target modulation and tumor growth delay at well tolerated doses when administered orally to SCID mice implanted with PTEN-deficient human tumor xenografts.

  13. Biological Systems for Hydrogen Photoproduction (Presentation)

    SciTech Connect

    Ghirardi, M. L.

    2012-05-01

    This presentation summarizes NREL biological systems for hydrogen photoproduction work for the DOE Hydrogen and Fuel Cells Program Annual Merit Review and Peer Evaluation Meeting, May 14-18, 2012. General goal is develop photobiological systems for large-scale, low cost and efficient H{sub 2} production from water (barriers AH, AI and AJ). Specific tasks are: (1) Address the O{sub 2} sensitivity of hydrogenases that prevent continuity of H{sub 2} photoproduction under aerobic, high solar-to-hydrogen (STH) light conversion efficiency conditions; and (2) Utilize a limited STH H{sub 2}-producing method (sulfur deprivation) as a platform to address or test other factors limiting commercial algal H{sub 2} photoproduction, including low rates due to biochemical and engineering mechanisms.

  14. Photoproduction of the Lambda*(1520) Hyperon

    SciTech Connect

    Z. W. Zhao, H. Y. Lu, L. Graham, K. Park, R. W. Gothe

    2010-08-01

    The photoproduction of the Lambda*(1520) on both the proton and neutron have been studied by using the CLAS eg3 run data set. The reactions are gammad-->K+Lambda*(n) and gammad-->K0Lambda*(p) with Lambda*-->pK-. Preliminary total and differential cross sections have been extracted in the photon energy region 1.75 GeVphotoproduction of Lambda*(1520) on the neutron is reported, and we will extend the results on the proton to higher energies than in previous studies.

  15. Studies on porphyrin photoproducts in solution, cells, and tumor tissue

    NASA Astrophysics Data System (ADS)

    Koenig, Karsten; Schneckenburger, Herbert; Rueck, Angelika C.; Koenig, Roland

    1994-07-01

    Light excitation of photosensitizing porphyrins leads to cytotoxic reactions. In addition, photobleaching and photoproduct formation occur indicating photosensitizer destruction. Photoproducts from hematoporphyrin (HP) fluoresce in aqueous solution at 642 nm, whereas photoproducts from protoporphyrin (PP) in hydrophobic environment emit around 670 nm and exhibit pronounced absorption at 665 nm. Photoproduct formation depends on singlet oxygen. The photoproducts exhibit faster fluorescence decay kinetics compared with nonirradiated porphyrins, as shown by time-grated spectroscopy and fluorescence decay measurements. Photoproduct fluorescence was observed during light exposure of cells and of tumor-bearing, nude mice, following administration of Hematoporphyrin Derivative (HpD), tetramethyl-HP, and PP. Photoconversion was also detected with naturally-occurring porphyrins (PP-producing bacteria) and ALA-simulated biosynthesis of PP in tumor tissue and in skin lesions of patients (psoriasis, mycosis fungoides). The efficiency of PDT with porphyrin photoproducts was found to be low in spite of the strong electronic transitions in the red spectral region.

  16. Photoproduction of charm particles at Fermilab

    SciTech Connect

    Cumalat, John P.

    1997-03-15

    A brief description of the Fermilab Photoproduction Experiment E831 or FOCUS is presented. The experiment concentrates on the reconstruction of charm particles. The FOCUS collaboration has participants from several Central American and Latin American institutions; CINVESTAV and Universidad Autonoma de Puebla from Mexico, University of Puerto Rico from the United States, and Centro Brasileiro de Pesquisas Fisicas in Rio de Janeiro from Brasil.

  17. Photoproduction of the phi (1020) near threshold

    SciTech Connect

    D.J. Tedeschi; M. Huertas

    2000-05-12

    Photoproduction of phi mesons at photon energies below 2 GeV were measured at the CLAS detector in Hall B of Thomas Jefferson National Accelerator Facility. Preliminary differential cross sections are reported from the proton near threshold. The measurement are extended to the region in the variable t where production mechanisms beyond that of diffraction are expected to become significant.

  18. Factors affecting the efficiency of carbon monoxide photoproduction in the St. Lawrence estuarine system (Canada).

    PubMed

    Zhang, Yong; Xie, Huixiang; Chen, Guohua

    2006-12-15

    This study examined the effects of water temperature and the origin (terrestrial vs marine) and light history of chromophoric dissolved organic matter (CDOM) on the apparent quantum yields of carbon monoxide (CO) photoproduction for water samples collected along a salinity gradient (salinity range: 0-33) in the St. Lawrence estuarine system (Canada). The solar insolation-weighted mean apparent quantum yield of CO (phico) decreased as much as fourfold with increasing salinity and showed a strong positive correlation with the dissolved organic carbon-specific absorption coefficient at 254 nm. This suggests that terrestrial CDOM is more efficient at photochemically producing CO than is marine algae-derived CDOM and that aromatic moieties are likely involved in this photoprocess. CDOM photobleaching, mainly at the very early stage, dramatically decreased phico (by up to 6.4 times) for low-salinity samples, but photobleaching had little effect on the most marine sample. For a 20 degree C increase in temperature, phico increased by approximately 70% for low-salinity samples and 30-40% for saline samples. This study demonstrates that water temperature, as well as the CDOM's origin and light history, strongly affect the efficiency of CO photoproduction. These factors should be taken into account in modeling the photochemical fluxes of CO and other related CDOM photoproducts on varying spatiotemporal scales. PMID:17256526

  19. Crystal structure of a prokaryotic (6-4) photolyase with an Fe-S cluster and a 6,7-dimethyl-8-ribityllumazine antenna chromophore.

    PubMed

    Zhang, Fan; Scheerer, Patrick; Oberpichler, Inga; Lamparter, Tilman; Krauß, Norbert

    2013-04-30

    The (6-4) photolyases use blue light to reverse UV-induced (6-4) photoproducts in DNA. This (6-4) photorepair was thought to be restricted to eukaryotes. Here we report a prokaryotic (6-4) photolyase, PhrB from Agrobacterium tumefaciens, and propose that (6-4) photolyases are broadly distributed in prokaryotes. The crystal structure of photolyase related protein B (PhrB) at 1.45 Å resolution suggests a DNA binding mode different from that of the eukaryotic counterparts. A His-His-X-X-Arg motif is located within the proposed DNA lesion contact site of PhrB. This motif is structurally conserved in eukaryotic (6-4) photolyases for which the second His is essential for the (6-4) photolyase function. The PhrB structure contains 6,7-dimethyl-8-ribityllumazine as an antenna chromophore and a [4Fe-4S] cluster bound to the catalytic domain. A significant part of the Fe-S fold strikingly resembles that of the large subunit of eukaryotic and archaeal primases, suggesting that the PhrB-like photolyases branched at the base of the evolution of the cryptochrome/photolyase family. Our study presents a unique prokaryotic (6-4) photolyase and proposes that the prokaryotic (6-4) photolyases are the ancestors of the cryptochrome/photolyase family.

  20. Hadronic form factors in kaon photoproduction

    SciTech Connect

    Syukurilla, L. Mart, T.

    2014-09-25

    We have revisited the effect of hadronic form factors in kaon photoproduction process by utilizing an isobaric model developed for kaon photoproduction off the proton. The model is able to reproduce the available experimental data nicely as well as to reveal the origin of the second peak in the total cross section, which was the main source of confusion for decades. Different from our previous study, in the present work we explore the possibility of using different hadronic form factors in each of the KΛN vertices. The use of different hadronic form factors, e.g. dipole, Gaussian, and generalized dipole, has been found to produce a more flexible isobar model, which can provide a significant improvement in the model.

  1. Exclusive photoproduction of the cascade (Xi) hyperon

    SciTech Connect

    John Price; Bernard Nefkens; Justin Ducote; John Goetz; et. Al.

    2004-09-01

    We report on the first measurement of exclusive {Xi}{sup -}(1321) hyperon photoproduction in {gamma}p {yields} K{sup +}K{sup +}{Xi}{sup -} for 3.2 < E{sub {gamma}} < 3.9 GeV. The final state is identified by the missing mass in p({gamma}, K{sup +}K{sup +})X measured with the CLAS detector at Jefferson Laboratory. We have detected a significant number of the ground-state {Xi}{sup -}(132)1/2{sup +}, and have estimated the total cross section for its production. We have also observed the first excited state {Xi}{sup -}(1530)3/2{sup +}. Photoproduction provides a copious source of {Xi}'s. We discuss the possibilities of a search for the recently proposed {Xi}{sub 5}{sup --} and {Xi}{sub 5}{sup +} pentaquarks.

  2. Fourier-transform infrared study of the photoactivation process of Xenopus (6-4) photolyase.

    PubMed

    Yamada, Daichi; Zhang, Yu; Iwata, Tatsuya; Hitomi, Kenichi; Getzoff, Elizabeth D; Kandori, Hideki

    2012-07-24

    Photolyases (PHRs) are blue light-activated DNA repair enzymes that maintain genetic integrity by reverting UV-induced photoproducts into normal bases. The flavin adenine dinucleotide (FAD) chromophore of PHRs has four different redox states: oxidized (FAD(ox)), anion radical (FAD(•-)), neutral radical (FADH(•)), and fully reduced (FADH(-)). We combined difference Fourier-transform infrared (FTIR) spectroscopy with UV-visible spectroscopy to study the detailed photoactivation process of Xenopus (6-4) PHR. Two photons produce the enzymatically active, fully reduced PHR from oxidized FAD: FAD(ox) is converted to semiquinone via light-induced one-electron and one-proton transfers and then to FADH(-) by light-induced one-electron transfer. We successfully trapped FAD(•-) at 200 K, where electron transfer occurs but proton transfer does not. UV-visible spectroscopy following 450 nm illumination of FAD(ox) at 277 K defined the FADH(•)/FADH(-) mixture and allowed calculation of difference FTIR spectra among the four redox states. The absence of a characteristic C=O stretching vibration indicated that the proton donor is not a protonated carboxylic acid. Structural changes in Trp and Tyr are suggested by UV-visible and FTIR analysis of FAD(•-) at 200 K. Spectral analysis of amide I vibrations revealed structural perturbation of the protein's β-sheet during initial electron transfer (FAD(•-) formation), a transient increase in α-helicity during proton transfer (FADH(•) formation), and reversion to the initial amide I signal following subsequent electron transfer (FADH(-) formation). Consequently, in (6-4) PHR, unlike cryptochrome-DASH, formation of enzymatically active FADH(-) did not perturb α-helicity. Protein structural changes in the photoactivation of (6-4) PHR are discussed on the basis of these FTIR observations. PMID:22747528

  3. 1 CFR 6.4 - Monthly list of sections affected.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 1 General Provisions 1 2012-01-01 2012-01-01 false Monthly list of sections affected. 6.4 Section 6.4 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER THE FEDERAL REGISTER INDEXES AND ANCILLARIES § 6.4 Monthly list of sections affected. A monthly list of sections of the Code...

  4. 1 CFR 6.4 - Monthly list of sections affected.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 1 General Provisions 1 2014-01-01 2012-01-01 true Monthly list of sections affected. 6.4 Section 6.4 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER THE FEDERAL REGISTER INDEXES AND ANCILLARIES § 6.4 Monthly list of sections affected. A monthly list of sections of the Code...

  5. 1 CFR 6.4 - Monthly list of sections affected.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 1 General Provisions 1 2013-01-01 2012-01-01 true Monthly list of sections affected. 6.4 Section 6.4 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER THE FEDERAL REGISTER INDEXES AND ANCILLARIES § 6.4 Monthly list of sections affected. A monthly list of sections of the Code...

  6. 43 CFR 6.4 - Action by Solicitor.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Action by Solicitor. 6.4 Section 6.4 Public Lands: Interior Office of the Secretary of the Interior PATENT REGULATIONS Inventions by Employees § 6.4 Action by Solicitor. (a) If an employee inventor requests pursuant to § 6.2(e), that...

  7. 1 CFR 6.4 - Monthly list of sections affected.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 1 General Provisions 1 2010-01-01 2010-01-01 false Monthly list of sections affected. 6.4 Section 6.4 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER THE FEDERAL REGISTER INDEXES AND ANCILLARIES § 6.4 Monthly list of sections affected. A monthly list of sections of the Code...

  8. 1 CFR 6.4 - Monthly list of sections affected.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 1 General Provisions 1 2011-01-01 2011-01-01 false Monthly list of sections affected. 6.4 Section 6.4 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER THE FEDERAL REGISTER INDEXES AND ANCILLARIES § 6.4 Monthly list of sections affected. A monthly list of sections of the Code...

  9. Status of Meson Photoproduction Experiments with CLAS

    SciTech Connect

    Pasyuk, Eugene A.

    2014-01-01

    A large part of the experimental program in Hall B of the Jefferson Lab is dedicated to baryon spectroscopy. Meson photoproduction experiments are essential part of this program. CEBAF Large Acceptance Spectrometer (CLAS) and availability of circularly and linearly polarized tagged photon beams in combination with longitudinally and transversely polarized frozen spin targets provide unique conditions for this type of experiments. For the first time, a complete or nearly complete measurement became possible and will allow model independent extraction of the reaction amplitude. The measurements were complete with both proton and deuteron targets. An overview of the collected experimental data will be presented.

  10. Higgs boson photoproduction at the LHC

    SciTech Connect

    Ducati, M. B. Gay; Silveira, G. G.

    2011-07-15

    We present the current development of the photoproduction approach for the Higgs boson with its application to pp and pA collisions at the LHC. We perform a different analysis for the Gap Survival Probability, where we consider a probability of 3% and also a more optimistic value of 10% based on the HERA data for dijet production. As a result, the cross section for the exclusive Higgs boson production is about 2 fb and 6 fb in pp collisions and 617 and 2056 fb for pPb collisions, considering the gap survival factor of 3% and 10%, respectively.

  11. Photoproduction of neutral pions off protons

    NASA Astrophysics Data System (ADS)

    Crede, V.; Sparks, N.; Wilson, A.; Anisovich, A. V.; Bacelar, J. C. S.; Bantes, R.; Bartholomy, O.; Bayadilov, D.; Beck, R.; Beloglazov, Y. A.; Castelijns, R.; Dutz, H.; Elsner, D.; Ewald, R.; Frommberger, F.; Funke, Chr.; Gregor, R.; Gridnev, A.; Gutz, E.; Hillert, W.; Hoffmeister, P.; Jaegle, I.; Junkersfeld, J.; Kalinowsky, H.; Kammer, S.; Klein, Frank; Klein, Friedrich; Klempt, E.; Kotulla, M.; Krusche, B.; Löhner, H.; Lopatin, I. V.; Lugert, S.; Menze, D.; Mertens, T.; Messchendorp, J. G.; Metag, V.; Nanova, M.; Nikonov, V. A.; Novinski, D.; Novotny, R.; Ostrick, M.; Pant, L. M.; van Pee, H.; Pfeiffer, M.; Roy, A.; Sarantsev, A. V.; Schadmand, S.; Schmidt, C.; Schmieden, H.; Schoch, B.; Shende, S.; Sokhoyan, V.; Süle, A.; Sumachev, V. V.; Szczepanek, T.; Thoma, U.; Trnka, D.; Varma, R.; Walther, D.; Wendel, Ch.

    2011-11-01

    Photoproduction of neutral pions has been studied with the CBELSA/TAPS detector in the reaction γp→pπ0 for photon energies between 0.85 and 2.50 GeV. The π0 mesons are observed in their dominant neutral decay mode: π0→γγ. For the first time, the differential cross sections cover the very forward region, θc.m.<60∘. A partial-wave analysis of these data within the Bonn-Gatchina framework observes the high-mass resonances G17(2190), D13(2080), and D15(2070).

  12. Antibaryon photoproduction using CLAS at Jefferson Lab

    NASA Astrophysics Data System (ADS)

    Phelps, W.; Guo, L.

    2016-05-01

    There is little known about baryon-antibaryon photoproduction mechanisms. Three reactions, γ p →p p p ¯, γ p →p p n ¯π-, and γ p →p n p ¯π+ have been investigated for the photon energy range of 3.95-5.45 GeV. The data were from the g12 run period taken with the CLAS detector using a liquid hydrogen target in Hall B at Thomas Jefferson National Accelerator Facility. This experiment had high statistics, with an integrated luminosity of 68 pb-1. General features of the data and preliminary cross sections are presented.

  13. Charm and Beauty in Photoproduction at HERA

    NASA Astrophysics Data System (ADS)

    Dobre, Monica

    2014-04-01

    The photoproduction of beauty and charm quarks at the ep collider HERA are presented. The b-quarks production was investigated in the bb → eeX' channel and the differential production cross section was measured as a function of the average transverse momentum of the beauty quarks down to the threshold. The cross section of D* meson decaying in the golden channel was determined both inclusively and in D*-tagged dijet events. Exploiting the characteristics of the heavy-flavoured hadron decays, beauty and charm quark cross sections were also measured in dijet events using secondary vertices or semi-muonic decays.

  14. {Lambda}(1520) photoproduction with Regge contribution

    SciTech Connect

    Nam, Seung-il; Yu, Byung-Geel; Kao, Chung-Wen

    2011-10-21

    In this talk, we report our recent progresses on the {Lambda}(1520) photoproduction using the effective Lagrangian approach. In addition to the tree-level Born diagrams, we take into account the Regge-trajectories for the possible strange-meson exchanges in the t channel. We compute the angular and energy dependences of the production process, including polarization observables, such as the photon-beam asymmetry and the polarization-transfer coefficients, resulting in good qualitative agreement with current experimental data. We also compute the K{sup -} angle distribution function in the Gottfried-Jackson frame, using the polarization-transfer coefficients in the z direction.

  15. Hydrogen photoproduction of A. Variabilis incorporated in a photobioreactor

    NASA Astrophysics Data System (ADS)

    Liu, Jian-Guo; Hall, David O.; Rao, Krishna K.; Tsygankov, Anatoly A.; Sveshnikov, Dmitry A.

    1998-03-01

    H2 photoproduction and nitrogenase activities in two strains of Anabaena variabilis marked wild type ATCC 29413 and mutant PK84 exposed to thermal stress (temperature higher than the normal incubation temperature of 30°C) were studied. Cultures of both strains collected from any interval of logarithmic growth phase exhibited high H2 photoproduction and nitrogenase activities when exposed to limited time heat shock during the assay process. In contrast, the algal H2 photoproduction rate of both strains fluctuated with long term thermal stress caused by increasing the growth temperature from 30°C to 36°C. The changes of nitrogenase (the key H2 photobiosynthetic enzyme) activities in the mutant PK84 showed variation tendency similar to that of H2 photoproduction during exposure to thermal stress, indicating that fluctuation of H2 photoproduction in the mutant was mainly due to the variation of nitrogenase activities. A temporary maximal H2 photoproduction in the mutant PK84 (wild type ATCC29413) was observed when cells grew at 36°C for 14 (6) days. However, the responses of nitrogenase activities in the wild type to thermal stress were not completely similar to those in the mutant in spite of similar variations of H2 photoproduction in both strains. The data obtained in these studies suggested that the activities of other enzymes (in the wild strain) involved in H2 photoproduction were affected by thermal stress since H2 photoproduction maximized or dropped to 0 without variation tendency similar to that of nitrogenase activities. Furthermore, an enhancement of H2 photoproduction speed of the mutant strain cultured in a 4.4 L laboratory photobioreactor was also observed when it was subjected to short time continuous charge of argon, and temperature rise. All these results indicated that high temperature plays an important role in the photo-autotrophic H2 photoproduction, and that long term thermal stress is unfavourable for net H2 photoproduction in both strains

  16. GlueX: Meson Spectroscopy in Photoproduction

    SciTech Connect

    Salgado, Carlos; Smith, Elton S.

    2014-03-01

    The goal of the GlueX experiment \\cite{gluex} is to provide crucial data to help understand the soft gluonic fields responsible for binding quarks in hadrons. Hybrid mesons, and in particular exotic hybrid mesons, provide the ideal laboratory for testing QCD in the confinement regime since these mesons explicitly manifest the gluonic degrees of freedom. Photoproduction is expected to be effective in producing exotic hybrids but there is little data on the photoproduction of light mesons. GlueX will use the new 12-GeV electron beam at Jefferson Lab to produce a 9-GeV beam of linearly polarized photons using the technique of coherent bremsstrahlung. A solenoid-based hermetic detector is under construction, which will be used to collect data on meson production and decays. These data will also be used to study the spectrum of conventional mesons, including the poorly understood excited vector mesons. This talk will give an update on the experiment as well as describe theoretical developments \\cite{Dudek:2011bn} to help understand how these data can provide insights into the fundamental theory of strong interactions.

  17. 15 CFR 6.4 - Adjustments to penalties.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6.4 Commerce and Foreign Trade Office of the Secretary of Commerce CIVIL MONETARY PENALTY INFLATION ADJUSTMENTS § 6.4 Adjustments to penalties. The civil monetary penalties provided by law within the... Sensing Policy Act of 1992, from $11,000 to $11,000. (2) 15 U.S.C. 5658(c), Land Remote Sensing Policy...

  18. 44 CFR 6.4 - Standards of accuracy.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Standards of accuracy. 6.4 Section 6.4 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF.... The system manager shall ensure that all records which are used by FEMA to make determinations...

  19. Photoproduction of the rho meson and its magnetic moments

    SciTech Connect

    Kaneko, Hiromi; Hosaka, Atsushi; Scholten, Olaf

    2011-10-21

    We study photoproduction of {rho} meson in a model of hidden local symmetry. We introduce the {rho} meson on a hidden gauge boson and phenomenological {rho} meson-nucleon Lagrangian is constructed respecting chiral symmetry. It turns out that the {sigma}-exchange interaction plays an important role in neutral {rho} meson photoproduction to reproduce the experimental cross sections. In charged {rho} meson photoproduction, the model takes into account the {rho} meson magnetic moments from the three-point vertex in the kinetic terms. We show that the magnetic moment of the charged {rho} meson has a significant effect on the total cross sections in proportion to the photon energies.

  20. Strangeness Photoproduction at the BGO-OD Experiment

    NASA Astrophysics Data System (ADS)

    Jude, T. C.; Alef, S.; Bayadilov, D.; Beck, R.; Becker, M.; Bella, A.; Bielefeldt, P.; Boese, S.; Braghieri, A.; Brinkmann, K.; Cole, P.; Curciarello, F.; De Leo, V.; Di Salvo, R.; Dutz, H.; Elsner, D.; Fantini, A.; Freyermuth, O.; Friedrich, S.; Frommberger, F.; Ganenko, V.; Gervino, G.; Ghio, F.; Giardina, G.; Goertz, S.; Gridnev, A.; Gutz, E.; Hammann, D.; Hannappel, J.; Hartmann, P.; Hillert, W.; Ignatov, A.; Jahn, R.; Joosten, R.; Klein, F.; Koop, K.; Krusche, B.; Lapik, A.; Levi Sandri, P.; Lopatin, I. V.; Mandaglio, G.; Messi, F.; Messi, R.; Metag, V.; Moricciani, D.; Mushkarenkov, A.; Nanova, M.; Nedorezov, V.; Novinskiy, D.; Pedroni, P.; Reitz, B.; Romaniuk, M.; Rostomyan, T.; Rudnev, N.; Scheluchin, G.; Schmieden, H.; Stugelev, A.; Sumachev, V.; Tarakanov, V.; Vegna, V.; Walther, D.; Watts, D.; Zaunick, H.; Zimmermann, T.

    BGO-OD is a newly commissioned experiment to investigate the internal structure of the nucleon, using an energy tagged bremsstrahlung photon beam at the ELSA electron facility. The setup consists of a highly segmented BGO calorimeter surrounding the target, with a particle tracking magnetic spectrometer at forward angles. BGO-OD is ideal for investigating meson photoproduction. The extensive physics programme for open strangeness photoproduction is introduced, and preliminary analysis presented.

  1. Model discrimination in pseudoscalar-meson photoproduction

    NASA Astrophysics Data System (ADS)

    Nys, J.; Ryckebusch, J.; Ireland, D. G.; Glazier, D. I.

    2016-08-01

    To learn about a physical system of interest, experimental results must be able to discriminate among models. We introduce a geometrical measure to quantify the distance between models for pseudoscalar-meson photoproduction in amplitude space. Experimental observables, with finite precision, map to probability distributions in amplitude space, and the characteristic width scale of such distributions needs to be smaller than the distance between models if the observable data are going to be useful. We therefore also introduce a method for evaluating probability distributions in amplitude space that arise as a result of one or more measurements, and show how one can use this to determine what further measurements are going to be necessary to be able to discriminate among models.

  2. Photoproduction of the Λ c charmed baryon

    NASA Astrophysics Data System (ADS)

    Alvarez, M. P.; Barate, R.; Bloch, D.; Bonamy, P.; Borgeaud, P.; Burchell, M.; Burmeister, H.; Brunet, J. M.; Calvino, F.; Cattaneo, M.; Crespo, J. M.; d'Almagne, B.; David, M.; DiCiaccio, L.; Dixon, J.; Druet, P.; Duane, A.; Engel, J. P.; Ferrer, A.; Filippas, T. A.; Fokitis, E.; Forty, R. W.; Foucault, P.; Gazis, E. N.; Gerber, J. P.; Giomataris, Y.; Hofmokl, T.; Katsoufis, E. C.; Koratzinos, M.; Krafft, C.; Lefievre, B.; Lemoigne, Y.; Lopez, A.; Lui, W. K.; Magneville, C.; Maltezos, A.; McEwen, J. G.; Papadopoulou, Th.; Pattison, B.; Poutot, D.; Primout, M.; Rahmani, H.; Roudeau, P.; Seez, C.; Six, J.; Strub, R.; Treille, D.; Triscos, P.; Tristram, G.; Villet, G.; Volte, A.; Wayne, M.; Websdale, D. M.; Wormser, G.; Zolnierowski, Y.; NA14/2 Collaboration

    1990-08-01

    In a photoproduction experiment using a mean photon energy of 100 GeV we have observed 29±8 Λ c( overlineΛ c) charmed-baryon and antibaryon decays in the pK-π + ( overlinepK +π -) final state. Quasi two-body final states do not contribite significantly to this channel. The mass of the Λ c was measured to be 2281.7±2.7±2.6 MeV/ c2 and its lifetime 0.18±0.03±0.03 ps. The ratio of {Λ c}/{D} production, measured in this experiment, is significantly greater than that predicted by photon-gluon fusion and using a Lund model to describe the hadronization. This excess cannot be completely accounted for in this model, even using a Λ c branching fraction in pK π as high as 5%.

  3. Photocouplings at the pole from pion photoproduction

    SciTech Connect

    Ronchen, D.; Doring, M.; Huang, F.; Haberzettl, H.; Haidenbauer, J.; Hanhart, C.; Krewald, S.; MeiBner, U. -G.; Nakayama, K.

    2014-06-24

    The reactions γp → π0p and γp → π+n are analyzed in a semi-phenomenological approach up to E ~ 2.3 GeV. Fits to differential cross section and single and double polarization observables are performed. A good overall reproduction of the available photoproduction data is achieved. The Julich2012 dynamical coupled-channel model -which describes elastic πN scattering and the world data base of the reactions πN → ηN, KΛ, and KΣ at the same time– is employed as the hadronic interaction in the final state. Furthermore, the framework guarantees analyticity and, thus, allows for a reliable extraction of resonance parameters in terms of poles and residues. In particular, the photocouplings at the pole can be extracted and are presented.

  4. Microbial degradation of thidiazuron and its photoproduct.

    PubMed

    Benezet, H J; Knowles, C O

    1982-01-01

    Degradation of the cotton defoliant thidiazuron and its photoproduct photothidiazuron by soil and thirteen species of microorganisms was examined. Aspergillus versicolor, Torula rosea, and Flavobacter sp. were most active in degrading thidiazuron. Unknown water-soluble metabolites and phenylurea were the major products. A. versicolor and Penicillium cyclopium were most active in degrading photothidiazuron. 4-Hydroxyphenylphotothidiazuron was the major organosoluble product formed by A. versicolor; phenylurea and an unidentified metabolite constituted the major organosoluble products from P. cyclopium. Both microbes also formed appreciable water-soluble metabolites. Radioactive carbon dioxide was formed from thidiazuron-aniline-14C by Oscillatoria sp. but not by Chlorella sp., suggesting that the former algal species utilized the defoliant as an energy source. PMID:7073312

  5. Microbial degradation of thidiazuron and its photoproduct.

    PubMed

    Benezet, H J; Knowles, C O

    1982-01-01

    Degradation of the cotton defoliant thidiazuron and its photoproduct photothidiazuron by soil and thirteen species of microorganisms was examined. Aspergillus versicolor, Torula rosea, and Flavobacter sp. were most active in degrading thidiazuron. Unknown water-soluble metabolites and phenylurea were the major products. A. versicolor and Penicillium cyclopium were most active in degrading photothidiazuron. 4-Hydroxyphenylphotothidiazuron was the major organosoluble product formed by A. versicolor; phenylurea and an unidentified metabolite constituted the major organosoluble products from P. cyclopium. Both microbes also formed appreciable water-soluble metabolites. Radioactive carbon dioxide was formed from thidiazuron-aniline-14C by Oscillatoria sp. but not by Chlorella sp., suggesting that the former algal species utilized the defoliant as an energy source.

  6. Meson photoproduction from the nucleon at CLAS

    SciTech Connect

    Daniel P. Watts

    2012-01-01

    The excitation spectrum of the nucleon provides a stringent constraint on the dynamics and interactions of its internal constituents and therefore probes the mechanism of confinement in the light quark sector. Our detailed knowlege of this excitation spectrum is poor, with many predicted states not yet observed in experiment and many 'established' states having poorly known properties. To address these shortcomings a worldwide effort is currently underway exploiting the latest generation of electron and photon beams in detailed studies of meson photoproduction from nucleon targets. A major contribution to this effort will come from the experimental programme at Jefferson Lab exploiting the frozen spin target (FROST) with the CLAS spectrometer. The status of this project will be presented along with preliminary results and analyses.

  7. Photocouplings at the pole from pion photoproduction

    DOE PAGESBeta

    Ronchen, D.; Doring, M.; Huang, F.; Haberzettl, H.; Haidenbauer, J.; Hanhart, C.; Krewald, S.; MeiBner, U. -G.; Nakayama, K.

    2014-06-24

    The reactions γp → π0p and γp → π+n are analyzed in a semi-phenomenological approach up to E ~ 2.3 GeV. Fits to differential cross section and single and double polarization observables are performed. A good overall reproduction of the available photoproduction data is achieved. The Julich2012 dynamical coupled-channel model -which describes elastic πN scattering and the world data base of the reactions πN → ηN, KΛ, and KΣ at the same time– is employed as the hadronic interaction in the final state. Furthermore, the framework guarantees analyticity and, thus, allows for a reliable extraction of resonance parameters in termsmore » of poles and residues. In particular, the photocouplings at the pole can be extracted and are presented.« less

  8. Photoproduction of heavy quarkonium at the ILC

    NASA Astrophysics Data System (ADS)

    Chen, Gu; Wu, Xing-Gang; Fu, Hai-Bing; Han, Hua-Yong; Sun, Zhan

    2014-08-01

    We study the photoproduction of the heavy quarkonium at the future International Linear Collider (ILC) within the nonrelativistic QCD theory. We focus on the production channel via the subprocess γγ→|[Q1(n)⟩+Q'+Q¯, where Q and Q' stand for the heavy c or b quark, respectively. |[Q Q1(n)⟩ stands for the color-singlet S-wave quarkonium, i.e., ηc(|[c1(S10)⟩), J/ψ(|[cc¯]1(S31)⟩), Bc(|[c1(S10)⟩), Bc*(|[cb¯]1(S31)⟩), ηb(|[b1(1S0)⟩), and Υ(|[bb¯]1(S31)⟩), respectively. To improve the calculation efficiency, we adopt the improved helicity amplitude approach to deal with the difficulty of calculating the expressions for the yields when the quark masses cannot be neglected. Total and differential photoproduction cross sections, together with their uncertainties, have been presented. It is noted that a sizable number of |cc¯⟩-charmonium and |cb¯⟩-quarkonium events can be generated at the ILC. More specifically, we predict (2.4-0.6+0.8)×106 ηc, (4.7-1.1+1.6)×106 J/ψ, (8.6-1.9+2.3)×103 Bc, (4.6-0.9+1.3)×104 Bc*, (6.6±1.2)×103 ηb, and (1.2±0.2)×103 ϒ events to be generated in one operation year at the ILC under the condition of √S =500 GeV and L ≃1036 cm-2s-1.

  9. DETERMINATION OF APPARENT QUANTUM YIELD SPECTRA FOR THE FORMATION OF BIOLOGICALLY LABILE PHOTOPRODUCTS

    EPA Science Inventory

    Quantum yield spectra for the photochemical formation of biologically labile photoproducts from dissolved organic matter (DOM) have not been available previously, although they would greatly facilitate attempts to model photoproduct formation rates across latitudinal, seasonal, a...

  10. Photoproduction of the K+ K0-(1750)

    SciTech Connect

    Mitchell, Ryan Edward

    2003-01-01

    While photoproduction has often been advertised as an important environment in which to study light meson spectroscopy, solid experimental results are sparse. In fact, beyond the relatively straightforward photoproduction of the {rho}, {omega}, and {phi} mesons, the few results of exclusive photoproduction that do exist are poorly understood, and several, perhaps, have even been misinterpreted. After extensively reviewing the sometimes tenuous history of the exclusive photoproduction of the ''{rho}{sup 1}(1600)'', the ''{omega}{pi}{sup 0}(1250)'', the ''{omega}(1650)'', and the ''K{sup +}K{sup -}(1750)'', new results from the E831/FOCUS photoproduction experiment at Fermilab are presented which address the interpretation of the K{sup +}K{sup -}(1750). This enhancement in low-p{sub T} K{sup +}K{sup -} pairs at a mass near 1750 MeV/c{sup 2} has been observed by several previous photoproduction experiments, but, despite several apparent inconsistencies, it has always been interpreted as the J{sup PC} = 1{sup --} {phi}(1680) meson. With nearly two orders of magnitude more events than any previous observation of the K{sup +}K{sup -}(1750), and based on precise measurements of its mass and width, and its absence from the K*K final state, the FOCUS data can finally render this interpretation implausible. In addition, several steps have been taken towards establishing a new interpretation. Based on limited angular analyses of its decay and the beam energy dependence of its production, they argue that, in the absence of any wild interference scenarios, the K{sup +}K{sup -}(1750) has J{sup PC} {ne} 1{sup --}, and, in fact, the most likely assignment appears to be 2{sup ++}. It is hoped that this work can help set the stage for future reevaluations and new insights in photoproduction.

  11. 41 CFR 51-6.4 - Military resale commodities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 41 Public Contracts and Property Management 1 2013-07-01 2013-07-01 false Military resale... PROCEDURES § 51-6.4 Military resale commodities. (a) Purchase procedures for ordering military resale commodities are available from the central nonprofit agencies. Authorized resale outlets (military...

  12. 41 CFR 51-6.4 - Military resale commodities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Military resale... PROCEDURES § 51-6.4 Military resale commodities. (a) Purchase procedures for ordering military resale commodities are available from the central nonprofit agencies. Authorized resale outlets (military...

  13. 34 CFR 6.4 - Central records; confidentiality.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Education Office of the Secretary, Department of Education INVENTIONS AND PATENTS (GENERAL) § 6.4 Central records; confidentiality. Central files and records shall be maintained of all inventions, patents, and... Department under such patents. Invention reports required from employees or others for the purpose...

  14. 34 CFR 6.4 - Central records; confidentiality.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Education Office of the Secretary, Department of Education INVENTIONS AND PATENTS (GENERAL) § 6.4 Central records; confidentiality. Central files and records shall be maintained of all inventions, patents, and... Department under such patents. Invention reports required from employees or others for the purpose...

  15. Photoproduction of ω mesons off the proton

    NASA Astrophysics Data System (ADS)

    Wilson, A.; Crede, V.; Anisovich, A. V.; Bacelar, J. C. S.; Bantes, B.; Bartholomy, O.; Bayadilov, D.; Beck, R.; Beloglazov, Y. A.; Brinkmann, K. T.; Castelijns, R.; Dutz, H.; Elsner, D.; Ewald, R.; Frommberger, F.; Fuchs, M.; Funke, Chr.; Gregor, R.; Gridnev, A.; Gutz, E.; Hannappel, J.; Hillert, W.; Hoffmeister, P.; Horn, I.; Jaegle, I.; Jude, T.; Junkersfeld, J.; Kalinowsky, H.; Kleber, V.; Klein, Frank; Klein, Friedrich; Klempt, E.; Kotulla, M.; Krusche, B.; Lang, M.; Löhner, H.; Lopatin, I. V.; Lugert, S.; Mertens, T.; Messchendorp, J. G.; Metag, V.; Nanova, M.; Nikonov, V. A.; Novinski, D.; Novotny, R.; Ostrick, M.; Pant, L. M.; van Pee, H.; Pfeiffer, M.; Roy, A.; Sarantsev, A. V.; Schmidt, C.; Schmieden, H.; Shende, S.; Sokhoyan, V.; Sparks, N.; Süle, A.; Sumachev, V. V.; Szczepanek, T.; Thoma, U.; Trnka, D.; Varma, R.; Walther, D.; Wendel, Ch.; Wiedner, U.

    2015-10-01

    The differential cross sections and unpolarized spin-density matrix elements for the reaction γp → pω were measured using the CBELSA/TAPS experiment for initial photon energies ranging from the reaction threshold to 2.5 GeV. These observables were measured from the radiative decay of the ω meson, ω →π0 γ. The cross sections cover the full angular range and show the full extent of the t-channel forward rise. The overall shape of the angular distributions in the differential cross sections and unpolarized spin-density matrix elements are in fair agreement with previous data. In addition, for the first time, a beam of linearly-polarized tagged photons in the energy range from 1150 MeV to 1650 MeV was used to extract polarized spin-density matrix elements. These data were included in the Bonn-Gatchina partial wave analysis (PWA). The dominant contribution to ω photoproduction near threshold was found to be the 3 /2+ partial wave, which is primarily due to the sub-threshold N (1720) 3 /2+ resonance. At higher energies, pomeron-exchange was found to dominate whereas π-exchange remained small. These t-channel contributions as well as further contributions from nucleon resonances were necessary to describe the entire dataset: the 1 /2-, 3 /2-, and 5 /2+ partial waves were also found to contribute significantly.

  16. Meson photoproduction and baryon resonances at MAMBO experiment

    NASA Astrophysics Data System (ADS)

    Romaniuk, Mariia

    2013-03-01

    Photoproduction of mesons within the framework of the MAMBO experiment (BGO-OD at Bonn plus MAMI at Mainz) was studied. The results on the operative work of the cryogenic H2/D2 target system during the last commissioning beam times at the March and June 2012 are shown. Investigation of the single charged pion photoproduction was provided using a polarized 3He target at the tagged photon facility of the MAMI accelerator. Unpolarized and helicity dependent cross sections are presented for channels γN → π±X in the Δ(1232) baryon resonance region.

  17. Spore Photoproduct Lyase: The Known, the Controversial, and the Unknown*

    PubMed Central

    Yang, Linlin; Li, Lei

    2015-01-01

    Spore photoproduct lyase (SPL) repairs 5-thyminyl-5,6-dihydrothymine, a thymine dimer that is also called the spore photoproduct (SP), in germinating endospores. SPL is a radical S-adenosylmethionine (SAM) enzyme, utilizing the 5′-deoxyadenosyl radical generated by SAM reductive cleavage reaction to revert SP to two thymine residues. Here we review the current progress in SPL mechanistic studies. Protein radicals are known to be involved in SPL catalysis; however, how these radicals are quenched to close the catalytic cycle is under debate. PMID:25477522

  18. Backward-meson photoproduction at SPring-8/LEPS

    SciTech Connect

    Sumihama, Mizuki

    2011-10-21

    Photoproductions of {pi}{sup 0} and {eta} mesons from protons were measured at backward angles. Bump structures were observed in both reaction, and a strong angular distribution of photon asymmetries was found in {pi}{sup 0} production. These structures are discussed connecting with baryon resonances.

  19. Photoproduction of η and η' mesons off protons

    NASA Astrophysics Data System (ADS)

    Crede, V.; McVeigh, A.; Anisovich, A. V.; Bacelar, J. C. S.; Bantes, R.; Bartholomy, O.; Bayadilov, D.; Beck, R.; Beloglazov, Y. A.; Castelijns, R.; Ehmanns, A.; Elsner, D.; Essig, K.; Ewald, R.; Fabry, I.; Fuchs, M.; Funke, Chr.; Gothe, R.; Gregor, R.; Gridnev, A.; Gutz, E.; Höffgen, St.; Hoffmeister, P.; Horn, I.; Jaegle, I.; Junkersfeld, J.; Kalinowsky, H.; Kammer, S.; Klein, Frank; Klein, Friedrich; Klempt, E.; Konrad, M.; Kotulla, M.; Krusche, B.; Langheinrich, J.; Löhner, H.; Lopatin, I. V.; Lotz, J.; Lugert, S.; Menze, D.; Mertens, T.; Messchendorp, J. G.; Metag, V.; Nanova, M.; Nikonov, V. A.; Novinski, D.; Novotny, R.; Ostrick, M.; Pant, L. M.; van Pee, H.; Pfeiffer, M.; Roy, A.; Sarantsev, A. V.; Schadmand, S.; Schmidt, C.; Schmieden, H.; Schoch, B.; Shende, S.; Sokhoyan, V.; Sparks, N.; Süle, A.; Sumachev, V. V.; Szczepanek, T.; Thoma, U.; Trnka, D.; Varma, R.; Walther, D.; Weinheimer, Ch.; Wendel, Ch.; Wilson, A.

    2009-11-01

    Total and differential cross sections for η and η'photoproduction off the proton have been determined with the CBELSA/TAPS detector for photon energies between 0.85 and 2.55 GeV. The η mesons are detected in their two neutral decay modes, η→γγ and η→3π0→6γ, and for the first time, cover the full angular range in cosθc.m. of the η meson. These new η photoproduction data are consistent with the earlier CB-ELSA results. The η' mesons are observed in their neutral decay to π0π0η→6γ and also extend the coverage in angular range.

  20. Polarization Measurements in Photoproduction with CEBAF Large Acceptance Spectrometer

    SciTech Connect

    E. Pasyuk

    2010-05-01

    A significant part of the experimental program in Hall-B of the Jefferson Lab is dedicated to the studies of the structure of baryons. CEBAF Large Acceptance Spectrometer (CLAS), availability of circularly and linearly polarized photon beams and recent addition of polarized targets provides remarkable opportunity for single, double and in some cases triple polarization measurements in photoproduction. An overview of the experiments will be presented.

  1. Kaon Photoproduction Near Threshold in Six Isospin Channels Revisited

    NASA Astrophysics Data System (ADS)

    Mart, T.

    2016-08-01

    In this talk I review the progress achieved in the investigation of kaon photoproduction on the nucleon near the production threshold. This investigation has been performed by using the so-called isobar model, which makes use of Feynman diagrams for the background terms and the Breit-Wigner multipoles for the resonance terms. The future prospect as well as application of this model in the hadronic sector are also briefly discussed.

  2. Correlations between D and Dbar mesons in high energy photoproduction

    SciTech Connect

    Erik E Gottschalk

    2002-11-13

    Over 7000 events containing a fully reconstructed D{bar D} pair have been extracted from data recorded by the FOCUS photoproduction experiment at Fermilab. Preliminary results from a study of correlations between D and {bar D} mesons are presented. Correlations are used to study perturbative QCD predictions and investigate non-perturbative effects. We also present a preliminary result on the production of {psi}(3770).

  3. Double pi(0) Photoproduction on the Proton at GRAAL.

    PubMed

    Assafiri, Y; Bartalini, O; Bellini, V; Bocquet, J P; Bouchigny, S; Capogni, M; Castoldi, M; D'Angelo, A; Didelez, J P; Di Salvo, R; Fantini, A; Fichen, L; Gervino, G; Ghio, F; Girolami, B; Giusa, A; Guidal, M; Hourany, E; Kouznetsov, V; Kunne, R; Laget, J-M; Lapik, A; Sandri, P Levi; Lleres, A; Moricciani, D; Nedorezov, V; Rebreyend, D; Randieri, C; Renard, F; Rudnev, N; Schaerf, C; Sperduto, M; Sutera, M; Turinge, A; Zucchiatti, A

    2003-06-01

    The double pi(0) photoproduction off the proton has been measured in the beam energy range of 0.65-1.5 GeV. The total and differential cross sections and the Sigma beam asymmetry were extracted. The total cross section measured for the first time in the third resonance region of the nucleon shows a prominent peak. The interpretation of these results by two independent theoretical models infers mostly the selective excitation of P11- and D13-nucleon resonances.

  4. Charm and bottom photoproduction at HERA with MC@NLO

    SciTech Connect

    Toll T.; Frixione, S.

    2011-12-01

    We apply the MC@NLO formalism to the production of heavy-quark pairs in pointlike photon-hadron collisions. By combining this result with its analogue relevant to hadron-hadron collisions, we obtain NLO predictions matched to parton showers for the photoproduction of Q{bar Q} pairs. We compare MC{at}NLO results to the measurements of c- and b-flavored hadron observables performed by the H1 and ZEUS Collaborations at HERA.

  5. Microbial ecotoxicity and mutagenicity of 1-hydroxypyrene and its photoproducts.

    PubMed

    Hwang, H M; Shi, X; Ero, I; Jayasinghe, A; Dong, S; Yu, H

    2001-11-01

    1-Hydroxypyrene (1-HP) is a carcinogenic and slightly water-soluble polycyclic aromatic hydrocarbon. Ecotoxicity and mutagenicity of 1-HP and its photoproducts, and the effect of Mn2+ and Cu2+ on their mutagenicity were measured with microbial assay in this study. The assay includes spread plate counting, direct counting, microbial mineralization of 14C-UL-D-glucose and Mutatox Test. At the concentration examined (0.8 microM), the photoproducts (after 1.5 h solar irradiation) of 1-HP inhibited microbial glucose mineralization activity (by 64%) after microbial assemblages of a local reservoir site were exposed for 1 day. However, heterotrophic bacteria were able to utilize 1-HP photoproducts as the growth substrates and increase viability counts by up to 4.75-folds. 1-HP exhibited positive response to Mutatox Test in both direct medium and S-9 medium, with the lowest observable effective concentration of 0.625 microM in the test with direct medium. After photolysis, 1-HP decreased its mutagenicity. Mn2+ (312.5 microM-5 mM) and Cu2+ (6.25-100 microM) themselves are not mutagenic. However, addition of the metal ions before or after photolysis modifies the light readings of 1-HP during the test. Therefore, presence of metal ions could affect the genotoxicity of 1-HP in aquatic environments, depending on timing of the addition. PMID:11680740

  6. Femtosecond formation dynamics of primary photoproducts of visual pigment rhodopsin.

    PubMed

    Smitienko, O A; Mozgovaya, M N; Shelaev, I V; Gostev, F E; Feldman, T B; Nadtochenko, V A; Sarkisov, O M; Ostrovsky, M A

    2010-01-01

    The coherent 11-cis-retinal photoisomerization dynamics in bovine rhodopsin was studied by femtosecond time-resolved laser absorption spectroscopy at 30-fs resolution. Femtosecond pulses of 500, 535, and 560 nm wavelength were used for rhodopsin excitation to produce different initial Franck-Condon states and relevant distinct values of the vibrational energy of the molecule in its electron excited state. Time evolution of the photoinduced rhodopsin absorption spectra was monitored after femtosecond excitation in the spectral range of 400-720 nm. Oscillations of the time-resolved absorption signals of rhodopsin photoproducts represented by photorhodopsin(570) with vibrationally-excited all-trans-retinal and rhodopsin(498) in its initial state with vibrationally-excited 11-cis-retinal were studied. These oscillations reflect the dynamics of coherent vibrational wave-packets in the ground state of photoproducts. Fourier analysis of these oscillatory components has revealed frequencies, amplitudes, and initial phases of different vibrational modes, along which the motion of wave-packets of both photoproducts occurs. The main vibrational modes established are 62, 160 cm(-1) and 44, 142 cm(-1) for photorhodopsin(570) and for rhodopsin(498), respectively. These vibrational modes are directly involved in the coherent reaction under the study, and their amplitudes in the power spectrum obtained through the Fourier transform of the kinetic curves depend on the excitation wavelength of rhodopsin.

  7. Recent results on eta and eta-prime photoproduction on the proton

    SciTech Connect

    Barry Ritchie

    2004-06-01

    The experimental situation on eta and eta' photoproduction on the proton is reviewed, emphasizing progress made since 2001. New preliminary results for eta' photoproduction on the proton from Jefferson Lab are presented. Experimental results are compared with several theoretical approaches, with an emphasis on consequences for understanding baryon spectroscopy.

  8. Resonance Raman spectroscopy of octopus rhodopsin and its photoproducts

    SciTech Connect

    Pande, C.; Pande, A.; Yue, K.T.; Callender, R.; Ebrey, T.G.; Tsuda, M.

    1987-08-11

    The authors report here the resonance Raman spectra of octopus rhodopsin and its photoproducts, bathorhodopsin and acid metarhodopsin. These studies were undertaken in order to make comparisons with the well-studied bovine pigments, so as to understand the similarities and the differences in pigment structure and photochemical processes between vertebrates and invertebrates. The flow method was used to obtain the Raman spectrum of rhodopsin at 13 /sup 0/C. The bathorhodopsin spectrum was obtained by computer subtraction of the spectra containing different photostationary mixtures of rhodopsin, isorhodopsin, hypsorhodopsin, and bathorhodopsin, obtained at 12 K using the pump-probe technique and from measurements at 80 K. Like their bovine counterparts, the Schiff base vibrational mode appears at approx. 1660 cm/sup -1/ in octopus rhodopsin and the photoproducts, bathorhodopsin and acid metarhodopsin, suggesting a proteonated Schiff base linkage between the chromophore and the protein. Differences between the Raman spectra of octopus rhodopsin and bathorhodopsin indicate that the formation of bathorhodopsin is associated with chromophore isomerization. This inference is substantiated by the chromophore chemical extraction data which show that, like the bovine system, octopus rhodopsin is an 11-cis pigment, while the photoproducts contain an all-trans pigment, in agreement with the previous work. The octopus rhodopsin and bathorhodopsin spectra show marked differences from their bovine counterparts in other respects, however. The differences are most dramatic in the structure-sensitive fingerprint and the HOOP regions. Thus, it appears that although the two species differ in the specific nature of the chromophore-protein interactions, the general process of visual transduction is the same.

  9. Primakoff effect in η-photoproduction off protons

    DOE PAGESBeta

    A. Sibirtsev; Haidenbauer, J.; Krewald, S.; MeiBner, U. -G.

    2010-03-26

    In this paper, we analyse data on forward η -meson photoproduction off a proton target and extract the η → γγ decay width utilizing the Primakoff effect. The hadronic amplitude that enters into our analysis is strongly constrained because it is fixed from a global fit to available γp → pη data for differential cross-sections and polarizations. Finally, we compare our results with present information on the two-photon η-decay from the literature. We provide predictions for future PrimEx experiments at Jefferson Laboratory in order to motivate further studies.

  10. Subleading corrections to parity-violating pion photoproduction

    SciTech Connect

    Barry Holstein; Michael Ramsey-Musolf; Steven Puglia; Shi-Lin Zhu

    2001-09-01

    We compute the photon asymmetry B{sub {gamma}} for near threshold parity violating (PV) pion photoproduction through sub-leading order. We show that sub-leading contributions involve a new combination of PV couplings not included in previous analyses of hadronic PV. We argue that existing constraints on the leading order contribution to B{sub {gamma}}--obtained from the PV {gamma}-decay of {sup 18}F--suggest that the impact of the subleading contributions may be more significant than expected from naturalness arguments.

  11. Recent N* results from photoproduction experiments at CLAS

    SciTech Connect

    D. Sokhan

    2011-10-01

    The recent breakthroughs in the technology of polarized targets have enabled a new generation of meson photo-production experiments to be carried out. A measurement of a full set of polarization observables off both polarized proton and neutron tar gets and in a large number of meson-production channels has come within sight. Such a measurement would very significantly reduce model-dependence in the analysis of the data and thus has the potential to resolve long-standing issues, such as the 'missing resonance' problem, and shed new light on the nucleon excitation spectrum. This has formed the motivation for the recent N* experimental programme of CLAS.

  12. Diffractive photoproduction of J/ψ at LHC energies

    NASA Astrophysics Data System (ADS)

    Gonçalves, V. P.; Moreira, B. D.; Navarra, F. S.

    2015-07-01

    In this work we calculate the rapidity distribution and the total cross section for the diffractive photoproduction of J/ψ in PbPb collisions in LHC energies. We use the color dipole formalism and different models for the forward dipole-proton scattering amplitude (N) which take into account nonlinear effects. Using always the same model for the wave function of the vector meson, we estimate the theoretical uncertainty related to the choice of the model for N. We compare our results with available data from ALICE [1, 2] and present our prediction for higher enegies.

  13. Photoproduction of a2(1320 ) in a Regge model

    NASA Astrophysics Data System (ADS)

    Wang, Xiao-Yun; Guskov, Alexey

    2016-04-01

    In this work, the photoproduction of a2(1320 ) off a proton target is investigated within an effective Lagrangian approach and the Regge model. The theoretical result indicates that the shapes of the total and differential cross sections of the γ p →a2+n reaction within the Feynman (isobar) model are much different from that of the Reggeized treatment. The obtained cross section is compared with the existing experimental results at low energies. The a2(1320 ) production cross section at high energies can be tested by the COMPASS experiment, which can provide important information for clarifying the role of the Reggeized treatment at that energy range.

  14. Photoproduction and Decay of Light Mesons in CLAS

    SciTech Connect

    Amaryan, Moskov Jamalovich

    2013-08-01

    We present preliminary experimental results on photoproduction and decay of light mesons measured with CLAS setup at JLAB . This include Dalitz decay of pseudoscalar and vector mesons, radiative decay of pseudoscalar mesons as well hadronic decays of pseudoscalar and vector mesons. The collected high statistics in some of decay channels exceeds the world data by an order of magnitude and some other decay modes are observed for the first time. It is shown how the CLAS data will improve the world data on transition form factors of light mesons, Dalitz plot analyses, branching ratios of rare decay modes and other fundamental properties potentially accessible through the light meson decays.

  15. Beauty photoproduction using decays into electrons at HERA

    SciTech Connect

    Chekanov, S.; Derrick, M.; Magill, S.; Musgrave, B.; Nicholass, D.; Repond, J.; Yoshida, R.; Mattingly, M. C. K.; Antonioli, P.; Bari, G.; Bellagamba, L.; Boscherini, D.; Bruni, A.; Bruni, G.; Cindolo, F.; Corradi, M.; Iacobucci, G.; Margotti, A.; Nania, R.; Polini, A.

    2008-10-01

    Photoproduction of beauty quarks in events with two jets and an electron associated with one of the jets has been studied with the ZEUS detector at HERA using an integrated luminosity of 120 pb{sup -1}. The fractions of events containing b quarks, and also of events containing c quarks, were extracted from a likelihood fit using variables sensitive to electron identification as well as to semileptonic decays. Total and differential cross sections for beauty and charm production were measured and compared with next-to-leading-order QCD calculations and Monte Carlo models.

  16. 12 CFR 6.4 - Capital measures and capital category definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 1 2012-01-01 2012-01-01 false Capital measures and capital category definitions. 6.4 Section 6.4 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY PROMPT CORRECTIVE ACTION Capital Categories § 6.4 Capital measures and capital category definitions. (a) Capital measures. For purposes of section 38...

  17. Polarization asymmetry zero in heavy quark photoproduction and leptoproduction

    NASA Astrophysics Data System (ADS)

    Brodsky, Stanley J.; Schmidt, Ivan

    1998-03-01

    We demonstrate two novel features of the sea-quark contributions to the polarized structure functions and photoproduction cross sections, a zero sum rule and a zero crossing point of the polarization asymmetry, which can be traced directly to the dynamics of the perturbative tree-graph gluon-splitting contributions. In particular, we show that the Born contribution of massive quarks arising from photon-gluon fusion gives zero contribution to the logarithmic integral over the polarization asymmetry ∫dν/νΔσ(ν,Q2) for any photon virtuality. The vanishing of this integral in the Bjorken scaling limit then implies a zero gluon-splitting Born contribution to the Gourdin-Ellis-Jaffe sum rule for polarized structure functions from massive sea quarks. The vanishing of the polarization asymmetry at or near the canonical position predicted by perturbative QCD provides an important tool for verifying the dominance of the photon-gluon fusion contribution to charm photoproduction and for validating the effectiveness of this process as a measure of the gluon polarization ΔG(x,Q2) in the nucleon. The displacement of the asymmetry zero from its canonical position is sensitive to the virtuality of the gluon in the photon-gluon fusion subprocess, and it can provide a measure of intrinsic and higher-order sea quark contributions. © 1998

  18. Photoproduction of ω mesons off nuclei and impact of polarization on the meson-nucleon interaction

    DOE PAGESBeta

    Chudakov, Eugene A.; Gevorkyan, Sergey; Somov, Alexander

    2016-01-25

    We consider photoproduction of ω mesons off complex nuclei to study interactions of transversely and longitudinally polarized vector mesons with nucleons. Whereas the total cross section for interactions of the transversely polarized vector mesons with nucleons σT = σ(VTN) can be obtained from coherent photoproduction, measurements of vector meson photoproduction in the incoherent region provide a unique opportunity to extract the not-yet-measured total cross section for longitudinally polarized mesons σL = σ(VLN). The predictions for the latter strongly depend on the theoretical approaches. Furthermore, this work is stimulated by the construction of the new experiment GlueX at Jefferson Lab, designedmore » to study the photoproduction of mesons in a large beam energy range up to 12 GeV.« less

  19. Pole positions and residues from pion photoproduction using the Laurent-Pietarinen expansion method

    NASA Astrophysics Data System (ADS)

    Švarc, Alfred; Hadžimehmedović, Mirza; Osmanović, Hedim; Stahov, Jugoslav; Tiator, Lothar; Workman, Ron L.

    2014-06-01

    We applied a new approach to determine the pole positions and residues from pion photoproduction multipoles. The method is based on a Laurent expansion of the partial-wave T matrices, with a Pietarinen series representing the regular part of energy-dependent and single-energy photoproduction solutions. The method is applied to multipole fits generated by the MAID and George Washington University SAID (GWU-SAID) groups. We show that the number and properties of poles extracted from photoproduction data correspond very well to results from πN elastic data and values cited by the Particle Data Group (PDG). The photoproduction residues provide new information for the electromagnetic current at the pole position, which are independent of background parametrizations, which is not the case for the Breit-Wigner representation. Finally, we present the photodecay amplitudes from the current MAID and SAID solutions at the pole for all four-star nucleon resonances below W =2 GeV.

  20. Eta photoproduction in a combined analysis of pion- and photon-induced reactions

    NASA Astrophysics Data System (ADS)

    Rönchen, D.; Döring, M.; Haberzettl, H.; Haidenbauer, J.; Meißner, U.-G.; Nakayama, K.

    2015-06-01

    The ηN final state is isospin-selective and thus provides access to the spectrum of excited nucleons without being affected by excited Δ states. To this end, the world database on eta photoproduction off the proton up to a center-of-mass energy of E ˜ 2.3 GeV is analyzed, including data on differential cross sections, and single- and double-polarization observables. The resonance spectrum and its properties are determined in a combined analysis of eta and pion photoproduction off the proton together with the reactions πN → πN, ηN, KΛ and KΣ. For the analysis, the so-called Jülich coupled-channel framework is used, incorporating unitarity, analyticity, and effective three-body channels. Parameters tied to photoproduction and hadronic interactions are varied simultaneously. The influence of recent MAMI T and F asymmetry data on the eta photoproduction amplitude is discussed in detail.

  1. Exclusive photoproduction of quarkonium at the LHC energies within the color dipole approach

    SciTech Connect

    Ducati, M. B. Gay; Griep, M. T.; Machado, M. V. T.

    2015-04-10

    The exclusive photoproduction of ψ(2S) meson was investigated and the coherent and the incoherent contributions were evaluated. The light-cone dipole formalism was considered in this analysis and predictions are done for PbPb collisions at the CERN-LHC energy of 2.76 TeV. A comparison is done to the recent ALICE Collaboration data for the ψ(1S) state photoproduction with good agreement.

  2. Nonresonant Background in Isobaric Models of Photoproduction of η-Mesons on Nucleons

    NASA Astrophysics Data System (ADS)

    Tryasuchev, V. A.; Alekseev, B. A.; Yakovleva, V. S.; Kondratyeva, A. G.

    2016-07-01

    Within the framework of isobaric models of pseudoscalar meson photoproduction, the nonresonant background of photoproduction of η-mesons on nucleons is investigated as a function of energy. A bound on the magnitude of the pseudoscalar coupling constant of the η-meson with a nucleon is obtained: g η NN 2 /4π ≤ 0.01, and a bound on vector meson exchange models is also obtained.

  3. Unitary constraints on charged pion photoproduction at large p⊥

    DOE PAGESBeta

    Laget, Jean-Marc

    2010-01-25

    Aroundmore » $$\\theta_{\\pi}=$$90$$^\\circ$$, the coupling to the $$\\rho^\\circ N$$ channel leads to a good accounting of the charged pion exclusive photoproduction cross section in the energy range 3 < Eγ < 10 GeV, where experimental data exist. Starting from a Regge Pole approach that successfully describes vector meson production, the singular part of the corresponding box diagrams (where the intermediate vector meson-baryon pair propagates on-shell) is evaluated without any further assumptions (unitarity). Such a treatment provides an explanation of the $$s^{-7}$$ scaling of the cross section. Furthermore, elastic rescattering of the charged pion improves the basic Regge pole model at forward and backward angles.« less

  4. The FOREST detector for meson photoproduction experiments at ELPH

    NASA Astrophysics Data System (ADS)

    Ishikawa, T.; Fujimura, H.; Fukasawa, H.; Hashimoto, R.; Ishida, T.; Kaida, S.; Kasagi, J.; Kawano, A.; Kuwasaki, S.; Maeda, K.; Miyahara, F.; Mochizuki, K.; Nakabayashi, T.; Nakamura, A.; Nawa, K.; Ogushi, S.; Okada, Y.; Okamura, K.; Onodera, Y.; Saito, Y.; Sakamoto, Y.; Sato, M.; Shimizu, H.; Sugai, H.; Suzuki, K.; Takahashi, S.; Tsuchikawa, Y.; Yamazaki, H.; Yonemura, H.

    2016-10-01

    An electromagnetic calorimeter complex, FOREST, has been constructed for meson photoproduction experiments at the Research Center for Electron Photon Science, Tohoku University. It consists of three types of calorimeters, which are made of pure cesium-iodide crystals, lead scintillating-fiber modules, and lead glass Cherenkov counters. Each calorimeter is equipped with a plastic scintillator hodoscope to identify charged particles. The design and performance of FOREST are described. The energy responses of test calorimeters have been investigated by using 100-800 MeV positron beams. The energy resolutions of the three calorimeters are found to be approximately 3%, 7%, and 5% for 1-GeV photons, respectively. A cryogenic hydrogen/deuterium target system fitted to the FOREST experiments and a newly developed data acquisition system are also presented.

  5. The Evolution of t dependence in Meson Photoproduction

    NASA Astrophysics Data System (ADS)

    Puentes, Daniel; Raue, Brian; Guo, Lei; Freese, Adam

    2016-03-01

    Studies of single-meson photoproduction off the proton over the past few decades have yielded tremendous amounts of data on differential cross sections. The wealth of data can be used to understand the exchange mechanisms responsible for the production of specific final-state hadrons. At low momentum transfer the differential cross section dσ / dt can be parameterized by the t-slope parameter b, where dσ / dt ~e-bt . We have determined b as a function of photon beam energy for the reactions γp -->K+Σ0 , γp -->K+ Λ , γp -->p ω , γp -->p η , γp -->pη' , γp -->pρ0 , γp -->pπ0 , and γp -->p ϕ . Preliminary results will be presented.

  6. Baryon Antibaryon Photoproduction using CLAS at Jefferson Lab

    NASA Astrophysics Data System (ADS)

    Phelps, William

    2015-04-01

    There is little known about the baryon antibaryon photoproduction mechanism. Three reactions, γp --> pp p , γp --> ppπ- n , and γp --> p p π+ n have been investigated for the photon energy range of 4.4-5.45 GeV. The data were from the g12 experiment taken with the CLAS detector using a liquid hydrogen target at Thomas Jefferson National Accelerator Facility. This experiment had high statistics, with an integrated luminosity of 68 pb-1. General features of the data for these three reactions will be shown. In particular, the angular and energy dependence of the antibaryons as well as the preliminary normalized yields will be presented. Also, preliminary partial wave analysis results for the p p system will be discussed.

  7. Baryon Antibaryon Photoproduction using CLAS at Jefferson Lab

    NASA Astrophysics Data System (ADS)

    Phelps, William; CLAS Collaboration

    2014-09-01

    There is little known about the baryon antibaryon photoproduction mechanism. Three reactions, γ p --> pp p , γp --> pp π- n , and γp --> p p π+ n have been investigated for the photon energy range of 4.4-5.45 GeV. The data were from the g12 experiment taken with the CLAS detector using a liquid hydrogen target at Thomas Jefferson National Accelerator Facility. This experiment had high statistics, with an integrated luminosity of 68 pb-1. General features of the data for these three reactions will be shown. In particular, the angular and energy dependence of the antibaryons as well as the preliminary normalized yields will be presented. Also, preliminary partial wave analysis results for the p p system will be discussed.

  8. Near-threshold photoproduction of Φ mesons from deuterium

    DOE PAGESBeta

    Qian, X.; Chen, W.; Gao, H.; Hicks, K.; Kramer, K.; Laget, J. M.; Mibe, T.; Qiang, Y.; Stepanyan, S.; Tedeschi, D. J.; et al

    2011-01-05

    In this report, we measure the differential cross section onmore » $$\\phi$$-meson photoproduction from deuterium near the production threshold for a proton using the CLAS detector and a tagged-photon beam in Hall B at Jefferson Lab. The measurement was carried out by a triple coincidence detection of a proton, $K^+$ and $K^-$ near the theoretical production threshold of 1.57 GeV. Moreover, the extracted differential cross sections $$\\frac{d\\sigma}{dt}$$ for the initial photon energy from 1.65-1.75 GeV are consistent with predictions based on a quasifree mechanism. Ultimately, this experiment establishes a baseline for a future experimental search for an exotic $$\\phi$$-N bound state from heavier nuclear targets utilizing subthreshold/near-threshold production of $$\\phi$$ mesons.« less

  9. Photoproduction of the phi (1020) near threshold in CLAS

    SciTech Connect

    Tedeschi, D J

    2002-06-01

    The differential cross section for the photoproduction of the phi(1020) near threshold (E_{gamma} - 1.57GeV ) is predicted to be sensitive to production mechanisms other than diffraction. However, the existing low energy data is of limited statistics and kinematical coverage. Complete measurements of phi meson production on the proton have been performed at the Thomas Jefferson National Accelerator Facility using a liquid hydrogen target and the CEBAF Large Acceptance Spectrometer (CLAS). The phi was identified by missing mass using a proton and positive kaon detected by CLAS in coincidence with an electron in the photon tagger. The energy of the tagged, bremsstrahlung photons ranged from phi-threshold to 2.4 GeV. A description of the data set and the differential cross section far (E_{gamma} = 2.0 GeV ) will be presented and compared with present theoretical calculations.

  10. Near-threshold photoproduction of Φ mesons from deuterium

    SciTech Connect

    Qian, X.; Chen, W.; Gao, H.; Hicks, K.; Kramer, K.; Laget, J. M.; Mibe, T.; Qiang, Y.; Stepanyan, S.; Tedeschi, D. J.; Xu, W.; Adhikari, K. P.; Amaryan, M.; Anghinolfi, M.; Ball, J.; Battaglieri, M.; Batourine, V.; Bedlinskiy, I.; Bellis, M.; Biselli, A. S.; Bookwalter, C.; Branford, D.; Briscoe, W. J.; Brooks, W. K.; Burkert, V. D.; Careccia, S. L.; Carman, D. S.; Cole, P. L.; Collins, P.; Crede, V.; D'Angelo, A.; Daniel, A.; Dashyan, N.; De Vita, R.; De Sanctis, E.; Deur, A.; Dey, B.; Dhamija, S.; Djalali, C.; Doughty, D.; Dupre, R.; Egiyan, H.; El Alaoui, A.; Eugenio, P.; Fegan, S.; Gabrielyan, M. Y.; Gevorgyan, N.; Gilfoyle, G. P.; Giovanetti, K. L.; Girod, F. X.; Goetz, J. T.; Gohn, W.; Gothe, R. W.; Graham, L.; Griffioen, K. A.; Guidal, M.; Guo, L.; Hafidi, K.; Hakobyan, H.; Hanretty, C.; Hassall, N.; Holtrop, M.; Ilieva, Y.; Ireland, D. G.; Jawalkar, S. S.; Jo, H. S.; Joo, K.; Keller, D.; Khandaker, M.; Khetarpal, P.; Kim, A.; Kim, W.; Klein, A.; Klein, F. J.; Konczykowski, P.; Kubarovsky, V.; Kuleshov, S. V.; Kuznetsov, V.; Livingston, K.; Martinez, D.; Mayer, M.; McAndrew, J.; McCracken, M. E.; McKinnon, B.; Meyer, C. A.; Mikhailov, K.; Mineeva, T.; Mirazita, M.; Mokeev, V.; Moreno, B.; Moriya, K.; Morrison, B.; Moutarde, H.; Munevar, E.; Nadel-Turonski, P.; Ni, A.; Niccolai, S.; Niculescu, I.; Niroula, M. R.; Osipenko, M.; Ostrovidov, A. I.; Paremuzyan, R.; Park, K.; Park, S.; Pereira, S. Anefalos; Pisano, S.; Pogorelko, O.; Pozdniakov, S.; Price, J. W.; Procureur, S.; Protopopescu, D.; Ricco, G.; Ripani, M.; Ritchie, B. G.; Rosner, G.; Rossi, P.; Sabatié, F.; Saini, M. S.; Salgado, C.; Schott, D.; Schumacher, R. A.; Seder, E.; Seraydaryan, H.; Sharabian, Y. G.; Smith, E. S.; Smith, G. D.; Sober, D. I.; Sokhan, D.; Stepanyan, S. S.; Stoler, P.; Strakovsky, I. I.; Strauch, S.; Taiuti, M.; Taylor, C. E.; Tkachenko, S.; Ungaro, M.; Vernarsky, B.; Vineyard, M. F.; Voutier, E.; Weinstein, L. B.; Weygand, D. P.; Wood, M. H.; Zachariou, N.; Zana, L.; Zhang, J.; Zhao, B.; Zhao, Z. W.

    2011-01-05

    In this report, we measure the differential cross section on $\\phi$-meson photoproduction from deuterium near the production threshold for a proton using the CLAS detector and a tagged-photon beam in Hall B at Jefferson Lab. The measurement was carried out by a triple coincidence detection of a proton, $K^+$ and $K^-$ near the theoretical production threshold of 1.57 GeV. Moreover, the extracted differential cross sections $\\frac{d\\sigma}{dt}$ for the initial photon energy from 1.65-1.75 GeV are consistent with predictions based on a quasifree mechanism. Ultimately, this experiment establishes a baseline for a future experimental search for an exotic $\\phi$-N bound state from heavier nuclear targets utilizing subthreshold/near-threshold production of $\\phi$ mesons.

  11. Photoproduction of {pi}{pi} Pairs off {sup 7}Li

    SciTech Connect

    Maghrbi, Yasser

    2011-10-21

    This paper reports on the quasi-free photoproduction of {pi}{sup 0}{pi}{sup 0} and {pi}{sup 0}{pi}{sup +/-} pairs from {sup 7}Li in view of the in-medium properties of hadrons. Measurements have been done using the CB/TAPS detector setup and the Glasgow photon tagging spectrometer for incident photon energies up to 820 MeV. At small invariant masses, an enhancement of the neutral invariant mass distributions is seen compared to the mixed charged channel and could be explained either by an in-medium modification of the {pi}{pi} interaction in the I = J = 0 channel or by effects related to the final state interactions.

  12. Electro- and Photoproduction of Vector Mesons at Jefferson Lab

    SciTech Connect

    Marco Battaglieri

    2002-09-01

    The total and differential cross section for exclusive electro- and photoproduction of vector mesons in the resonance region and above (1.6 < W < 2.9 GeV) was measured at Jefferson Laboratory in a wide kinematic range (0 < Q2 < 4 GeV2 and 0< -t < 5 GeV2). The measurement of the total and differential cross section down to 100 pb/GeV2 with the full kinematic coverage, was possible for the first time thanks to the combination of the 100% duty cycle of CEBAF and the large acceptance of the CLAS detector. The main results from the CLAS Collaboration activity in this field will be presented and discussed.

  13. Determining pseudoscalar meson photoproduction amplitudes from complete experiments

    SciTech Connect

    A. M. Sandorfi, S. Hoblit, H. Kamano, T.-S. H. Lee

    2011-05-01

    In preparation for a new generation of complete experiments with the goal of performing a high precision extraction of pseudoscalar meson photo-production amplitudes, we present expressions that allow the direct numerical calculation of matrix elements with arbitrary spin projections from Chew-Goldberger-Low-Nambu (CGLN) amplitudes. We use this numerical tool to verify the most general analytic form of the cross section, dependent upon the three polarization vectors of the beam, target and recoil baryon, including all single, double and triple-polarization terms involving 16 spin-dependent observables. Analytic expressions that determine the recoil baryon polarization are presented, together with examples of their potential use with quasi-4? detectors to deduce observables. We assemble the analytic equations relating the 16 experimental observables and the CGLN amplitudes and use our independent method of numerical evaluation to resolve sign differences that exist in the literature.

  14. Baryon Antibaryon Photoproduction using CLAS at Jefferson Lab

    NASA Astrophysics Data System (ADS)

    Phelps, William; CLAS Collaboration

    2013-10-01

    There is little known about the baryon antibaryon production mechanism. The following reactions were looked at, γp -->pp p , γp -->ppπ- n , and γp -->p p π+ n. For these reactions the photon energies that were selected were from 4.4-5.45 GeV. The data were from the g12 experiment taken with the CLAS detector using a liquid hydrogen target at Thomas Jefferson National Accelerator Facility. This experiment had high statistics, with a luminosity of 68 pb-1. Features of the data such as invariant mass spectra, missing mass spectra, and angular distributions necessary for the analysis will be shown. In addition, a first observation of antineutrons in photoproduction in the missing mass spectra of γp -->ppπ- n will also be shown.

  15. Photoproduction of hydrogen by membranes of green photosynthetic bacteria

    SciTech Connect

    Bernstein, J D; Olson, J M

    1980-01-01

    Photoproduction of H/sub 2/ from ascorbate by unit-membrane vesicles from Chlorobium limicola f. thiosulfatophilum was achieved with a system containing gramicidin D, tetramethyl-p-phenylenediamine, methyl viologen, dithioerythritol, Clostridium hydrogenase, and an oxygen-scavenging mixture of glucose, glucose oxidase, ethanol, and catalase. Maximum quantum yield was less than one percent. Half maximum rate of H/sub 2/ production occurred at a white-light intensity of approximately 0.15 cm/sup -2/. The reaction was inhibited completely by 0.3% sodium dodecylbenzene sulfonate, 1% Triton X-100, or preheating the vesicles at 100/sup 0/C for 5 minutes. Low concentrations (0.01 and 0.05%) of Triton X-100 about doubled the reaction rate.

  16. A Radical Transfer Pathway in Spore Photoproduct Lyase

    PubMed Central

    Yang, Linlin; Nelson, Renae S.; Benjdia, Alhosna; Lin, Gengjie; Telser, Joshua; Stoll, Stefan; Schlichting, Ilme; Li, Lei

    2013-01-01

    Spore photoproduct lyase (SPL) repairs a covalent UV-induced thymine dimer, spore photoproduct (SP), in germinating endospores and is responsible for endospores’ strong UV resistance. SPL is a radical SAM enzyme, which uses a [4Fe-4S]1+ cluster to reduce the S-adenosyl-L-methionine (SAM), generating a catalytic 5′-deoxyadenosyl radical (5′-dA•). This in turn abstracts an H atom from SP, generating an SP radical that undergoes β scission to form a repaired 5′-thymine and a 3′-thymine allylic radical. Recent biochemical and structural data suggest that a conserved cysteine donates an H atom to the thymine radical, resulting in a putative thiyl radical. Here we present structural and biochemical data which suggest that two conserved tyrosines are also critical in enzyme catalysis. One (Y99(Bs) in Bacillus subtilis SPL) is downstream of the cysteine, suggesting that SPL uses a novel hydrogen atom transfer (HAT) pathway with a pair of cysteine-tyrosine residues to regenerate SAM. The other tyrosine (Y97(Bs)) has a structural role to facilitate SAM binding; it may also contribute to the SAM regeneration process by interacting with the putative •Y99(Bs) and/or 5′-dA• intermediates to lower the energy barrier for the second H-abstraction step. Our results indicate that SPL is the first member of the radical SAM superfamily (comprising more than 44,000 members) to bear a catalytically operating HAT chain. PMID:23607538

  17. 38 CFR 6.4 - Proof of age, relationship and marriage.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2012-07-01 2012-07-01 false Proof of age, relationship and marriage. 6.4 Section 6.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS....4 Proof of age, relationship and marriage. Whenever it is necessary for a claimant to prove...

  18. 38 CFR 6.4 - Proof of age, relationship and marriage.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2014-07-01 2014-07-01 false Proof of age, relationship and marriage. 6.4 Section 6.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS....4 Proof of age, relationship and marriage. Whenever it is necessary for a claimant to prove...

  19. 38 CFR 6.4 - Proof of age, relationship and marriage.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2013-07-01 2013-07-01 false Proof of age, relationship and marriage. 6.4 Section 6.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS....4 Proof of age, relationship and marriage. Whenever it is necessary for a claimant to prove...

  20. 38 CFR 6.4 - Proof of age, relationship and marriage.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false Proof of age, relationship and marriage. 6.4 Section 6.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS UNITED STATES GOVERNMENT LIFE INSURANCE Beneficiary of United States Government Life Insurance §...

  1. 38 CFR 6.4 - Proof of age, relationship and marriage.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., relationship and marriage. 6.4 Section 6.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS....4 Proof of age, relationship and marriage. Whenever it is necessary for a claimant to prove age, relationship or marriage, the provisions of 38 U.S.C. 103(c) and Part 3 this chapter will be followed....

  2. Genetics of human sensitivity to ultraviolet radiation

    NASA Astrophysics Data System (ADS)

    Cleaver, James E.

    1994-07-01

    the major human health effects of solar and artificial UV light occur from the UVB and UVC wavelength ranges and involve a variety of short-term and long-term deleterious changes to the skin and eyes. the more important initial damage to cellular macromolecules involves dimerization of adjacent pyrimidines in DNA to produce cyclobutane pyrimidine dimes, (6-4) pyrimidine- pyrimidone, and (6-4) dewar photoproducts. these photoproducts can be repaired by a genetically regulated enzyme system (nucleotide excision repair) which removes oligonucleotides 29-30 nucleotides long that contain the photoproducts, and synthesizes replacement patches. At least a dozen gene products are involved in the process of recognizing photoproducts in DNA, altering local DNA helicity and cleaving the polynucleotide chain at defined positions either side of a photoproduct. Hereditary mutations in many of these genes are recognized in the human genetic disorders xeroderma pigmentosum (XP), Cockayne syndrome (CS), and trichothiodystrophy (TTD). Several of the gene products have other functions involving the regulation of gene transcription which accounts for the complex clinical presentation of repair deficient diseases that involve sensitivity of the skin and eyes to UV light, increased solar carcinogenesis (in XP), demyelination, and ganglial calcification (in CS), hair abnormalities (in TTD), and developmental and neurological abnormalities

  3. Determining pseudoscalar meson photoproduction amplitudes from complete experiments

    NASA Astrophysics Data System (ADS)

    Sandorfi, A. M.; Hoblit, S.; Kamano, H.; Lee, T.-S. H.

    2011-05-01

    A new generation of complete experiments is focused on a high precision extraction of pseudoscalar meson photoproduction amplitudes. Here, we review the development of the most general analytic form of the cross section, dependent upon the three polarization vectors of the beam, target and recoil baryon, including all single-, double- and triple-polarization terms involving 16 spin-dependent observables. We examine the different conventions that have been used by different authors, and we present expressions that allow the direct numerical calculation of any pseudoscalar meson photoproduction observables with arbitrary spin projections from the Chew-Goldberger-Low-Nambu amplitudes. We use this numerical tool to clarify apparent sign differences that exist in the literature, in particular with the definitions of six double-polarization observables. We also present analytic expressions that determine the recoil baryon polarization, together with examples of their potential use with quasi-4π detectors to deduce observables. As an illustration of the use of the consistent machinery presented in this review, we carry out a multipole analysis of the γp → K+Λ reaction and examine the impact of recently published polarization measurements. When combining data from different experiments, we utilize the Fierz identities to fit a consistent set of scales. In fitting multipoles, we use a combined Monte Carlo sampling of the amplitude space, with gradient minimization, and find a shallow χ2 valley pitted with a very large number of local minima. This results in broad bands of multipole solutions that are experimentally indistinguishable. While these bands have been noticeably narrowed by the inclusion of new polarization measurements, many of the multipoles remain very poorly determined, even in sign, despite the inclusion of data on eight different observables. We have compared multipoles from recent PWA codes with our model-independent solution bands and found that such

  4. Study of the two pion final state photoproduction on deuterium

    SciTech Connect

    Graham, Lewis; Gothe, Ralf; Park, Kijun; Smith, Elton

    2010-08-05

    Understanding the structure of baryons in terms of the fundamental interaction of the constituent quarks and gluons is one of the challenges in strong interaction physics. This interaction is governed by Quantum Chromodynamics (QCD). However, solutions of this theory in the non-perturbative domain of the interaction are extremely difficult to achieve. In inelastic electron scattering, very little is known about exclusive hadron production purely contributed to a lack of knowledge. The {gamma}N interaction is recognized for being a powerful method for investigating hadrons and the mysteries that still exist within the strong interaction. From reactions with the nucleon, the strong interaction can be tested through the amplitudes of the N and {Delta} resonances. More specifically, if an electromagnetic interaction is well known then the intermediate resonance states may be evaluated through pion photoproduction. To gain more detailed insight into this interaction, we look to probe the baryon structure of {Delta} and the meson structure of the pion through photon scattering off a deuteron producing two pions in the final state. The photoproduction processes on the deuteron will be used to investigate known baryon resonances in the proton-pion channel. The two pion final state will be investigated for unraveling new information in to the rho decay at threshold. We want to explore both final states interactions to search for ''missing'' states that are predicted by quark models but have not yet been found experimentally. Using the CEBAF Large Acceptance Spectrometer (CLAS), the hadronic products are detected in coincidence with the scattered photon. This makes it possible to measure the differential cross section and the decay angular distribution for the production of two and three pion final states. The measured cross sections will contribute significantly and push the knowledge of the strong interaction to the next level. We propose to use the CEBAF Large Acceptance

  5. Study of the two pion final state photoproduction on deuterium

    SciTech Connect

    Lewis Graham, Kijun Park, Ralf Gothe, Elton Smith

    2010-08-01

    Understanding the structure of baryons in terms of the fundamental interaction of the constituent quarks and gluons is one of the challenges in strong interaction physics. This interaction is governed by Quantum Chromodynamics (QCD). However, solutions of this theory in the non-perturbative domain of the interaction are extremely difficult to achieve. In inelastic electron scattering, very little is known about exclusive hadron production purely contributed to a lack of knowledge. The gammaN interaction is recognized for being a powerful method for investigating hadrons and the mysteries that still exist within the strong interaction. From reactions with the nucleon, the strong interaction can be tested through the amplitudes of the N and Delta resonances. More specifically, if an electromagnetic interaction is well known then the intermediate resonance states may be evaluated through pion photoproduction. To gain more detailed insight into this interaction, we look to probe the baryon structure of Delta and the meson structure of the pion through photon scattering off a deuteron producing two pions in the final state. The photoproduction processes on the deuteron will be used to investigate known baryon resonances in the proton-pion channel. The two pion final state will be investigated for unraveling new information in to the rho decay at threshold. We want to explore both final states interactions to search for “missing” states that are predicted by quark models but have not yet been found experimentally. Using the CEBAF Large Acceptance Spectrometer (CLAS), the hadronic products are detected in coincidence with the scattered photon. This makes it possible to measure the differential cross section and the decay angular distribution for the production of two and three pion final states. The measured cross sections will contribute significantly and push the knowledge of the strong interaction to the next level. We propose to use the CEBAF Large Acceptance

  6. Determining pseudoscalar meson photoproduction amplitudes from complete experiments

    SciTech Connect

    Sandorfi, A.M.; Hoblit, S.; Sandorfi,A.M.; Hoblit,S.; Kamano,H.; Lee,T-S.H.

    2011-04-06

    A new generation of complete experiments is focused on a high precision extraction of pseudoscalar meson photoproduction amplitudes. Here, we review the development of the most general analytic form of the cross section, dependent upon the three polarization vectors of the beam, target and recoil baryon, including all single-, double- and triple-polarization terms involving 16 spin-dependent observables. We examine the different conventions that have been used by different authors, and we present expressions that allow the direct numerical calculation of any pseudoscalar meson photoproduction observables with arbitrary spin projections from the Chew-Goldberger-Low-Nambu amplitudes. We use this numerical tool to clarify apparent sign differences that exist in the literature, in particular with the definitions of six double-polarization observables. We also present analytic expressions that determine the recoil baryon polarization, together with examples of their potential use with quasi-4{pi} detectors to deduce observables. As an illustration of the use of the consistent machinery presented in this review, we carry out a multipole analysis of the {gamma}p {yields} K{sup +}{Lambda} reaction and examine the impact of recently published polarization measurements. When combining data from different experiments, we utilize the Fierz identities to fit a consistent set of scales. In fitting multipoles, we use a combined Monte Carlo sampling of the amplitude space, with gradient minimization, and find a shallow {chi}{sup 2} valley pitted with a very large number of local minima. This results in broad bands of multipole solutions that are experimentally indistinguishable. While these bands have been noticeably narrowed by the inclusion of new polarization measurements, many of the multipoles remain very poorly determined, even in sign, despite the inclusion of data on eight different observables. We have compared multipoles from recent PWA codes with our model

  7. Carbon Monoxide Photoproduction from Particles and Solutes in the Delaware Estuary under Contrasting Hydrological Conditions.

    PubMed

    Song, Guisheng; Richardson, John D; Werner, James P; Xie, Huixiang; Kieber, David J

    2015-12-15

    Full-spectrum, ultraviolet (UV), and visible broadband apparent quantum yields (AQYs) for carbon monoxide (CO) photoproduction from chromophoric dissolved organic matter (CDOM) and particulate organic matter (POM) were determined in the Delaware Estuary in two hydrologically contrasting seasons in 2012: an unusually low flow in August and a storm-driven high flow in November. Average AQYs for CDOM and POM in November were 10 and 16 times the corresponding AQYs in August. Maximum AQYs in November occurred in a midestuary particle absorption maximum zone. Although POM AQYs were generally smaller than CDOM AQYs, the ratio of the former to the latter increased substantially from the UV to the visible. In both seasons, UV solar radiation was the primary driver for CO photoproduction from CDOM whereas visible light was the principal contributor to POM-based CO photoproduction. CDOM dominated CO photoproduction in the uppermost water layer while POM prevailed at deeper depths. On a depth-integrated basis, the Delaware Estuary shifted from a CDOM-dominated system in August to a POM-dominated system in November with respect to CO photoproduction. This study reveals that flood events may enhance photochemical cycling of terrigenous organic matter and switch the primary photochemical driver from CDOM to POM. PMID:26506215

  8. Diffractive Higgs boson photoproduction in ultraperipheral collisions at LHC

    SciTech Connect

    Gay Ducati, M. B.; Silveira, G. G.

    2010-10-01

    A new production mechanism for the standard model Higgs boson in ultraperipheral collisions at the LHC, which allows central exclusive diffractive production by double pomeron exchange in photon-proton processes, is presented. The Higgs boson is centrally produced by gluon fusion with two large rapidity gaps emerging in the final state, being the main experimental signature for this process. As already studied for Pomeron-Pomeron and two-photon processes, the Higgs boson photoproduction is studied within this new mechanism in proton-proton (pp) and proton-nucleus (pA) collisions, where each system has a different dynamics to be taken into account. As a result, this mechanism predicts a production cross section for pp collisions of about 1.8 fb, which is similar to that obtained in Pomeron-Pomeron processes. Besides, in pPb collisions the cross sections have increased to about 0.6 pb, being comparable with the results of two-photon processes in pAu collisions. Therefore, as the rapidity gap survival probability is an open question in high-energy physics, an analysis for different values of this probability shows how competitive the mechanisms are in the LHC kinematical regime.

  9. High-energy photoproduction of rho and phi vector mesons

    SciTech Connect

    Callahan, P.H.

    1983-01-01

    We have studied the photoproduction of rho and phi vector mesons from hydrogen in the Fermilab broad band neutral beam. Forward going two particle final states were detected in a multiparticle spectrometer consisting of two analyzing magnets, a multiwire-proportional-chamber tracking system and a particle identification system. Recoil protons and target fragments were observed in a recoil detector which surrounded the target. The total elastic cross-sections were measured to be independent of energy at the 10% level from 35 to 225 GeV at 10.6 ..mu..b for the rho and from 35 to 165 GeV at 0.64 ..mu..b for the phi. The elastic differential cross-sections were also measured. Approximately 20% of the diffractive rho and phi events were found to be inelastic from an analysis of the recoil detector. The t-dependence of the fraction of diffractive events which are inelastic for both the phi and the psi are consistent with a universal function determined from the rho data.

  10. Photoproduction of $\\pi^+ \\pi^-$ meson pairs on the proton

    SciTech Connect

    Marco A. Battaglieri; DeVita, Raffaella; Szczepaniak, Adam P.

    2009-10-01

    The exclusive reaction $\\gamma p \\to p \\pi^+ \\pi^-$ was studied in the photon energy range 3.0 - 3.8 GeV and momentum transfer range $0.4<-t<1.0$ GeV$^2$. Data were collected with the CLAS detector at the Thomas Jefferson National Accelerator Facility. In this kinematic range the integrated luminosity was about 20 pb$^{-1}$. The reaction was isolated by detecting the $\\pi^+$ and proton in CLAS, and reconstructing the $\\pi^-$ via the missing-mass technique. Moments of the di-pion decay angular distributions were derived from the experimental data. Differential cross sections for the $S$, $P$, and $D$-waves in the $M_{\\pi^+\\pi^-}$ mass range $0.4-1.4$ GeV were derived performing a partial wave expansion of the extracted moments. Besides the dominant contribution of the $\\rho(770)$ meson in the $P$-wave, evidence for the $f_0(980)$ and the $f_2(1270)$ mesons was found in the $S$ and $D$-waves, respectively. The differential production cross sections $d\\sigma/dt$ for individual waves in the mass range of the above-mentioned mesons were extracted. This is the first time the $f_0(980)$ has been measured in a photoproduction experiment.

  11. Dynamical Coupled-channels Effects on Pion Photoproduction

    SciTech Connect

    Julia-Diaz, B; Lee, T -S. H.; Matsuyama, A; Sato, T; Smith, L C

    2007-12-18

    The electromagnetic pion production reactions are investigated within the dynamical coupled-channels model developed in {\\bf Physics Reports, 439, 193 (2007)}. The meson-baryon channels included in this study are $\\gamma N$, $\\pi N$, $\\eta N$, and the $\\pi\\Delta$, $\\rho N$ and $\\sigma N$ resonant components of the $\\pi\\pi N$ channel. With the hadronic parameters of the model determined in a recent study of $\\pi N$ scattering, we show that the pion photoproduction data up to the second resonance region can be described to a very large extent by only adjusting the bare $\\gamma N \\rightarrow N^*$ helicity amplitudes, while the non-resonant electromagnetic couplings are taken from previous works. It is found that the coupled-channels effects can contribute about 10 - 20 $\\%$ of the production cross sections in the $\\Delta$ (1232) resonance region, and can drastically change the magnitude and shape of the cross sections in the second resonance region. The importance of the off-shell effects in a dynamical approach is also demonstrated. The meson cloud effects as well as the coupled-channels contributions to the $\\gamma N \\rightarrow N^*$ form factors are found to be mainly in the low $Q^2$ region. For the magnetic M1 $\\gamma N \\rightarrow \\Delta$ (1232) form factor, the results are close to that of the Sato-Lee Model. Necessary improvements to the model and future developments are discussed.

  12. Dynamical coupled-channels effects on pion photoproduction

    SciTech Connect

    Julia-Diaz, B.; Lee, T.-S. H.; Matsuyama, A.; Sato, T.; Smith, L. C.

    2008-04-15

    The electromagnetic pion production reactions are investigated within the dynamical coupled-channels model developed by Matsuyama, Sato, and Lee [Phys. Rep. 439, 193 (2007)]. The meson-baryon channels included in this study are {gamma}N,{pi}N,{eta}N, and the {pi}{delta},{rho}N, and {sigma}N resonant components of the {pi}{pi}N channel. With the hadronic parameters of the model determined in a recent study of {pi}N scattering, we show that the pion photoproduction data up to the second resonance region can be described to a very large extent by only adjusting the bare {gamma}N{yields}N* helicity amplitudes, while the nonresonant electromagnetic couplings are taken from previous works. It is found that the coupled-channels effects can contribute about 30-40 % of the production cross sections in the {delta} (1232) resonance region, and can drastically change the magnitude and shape of the cross sections in the second resonance region. The importance of the loop-integrations in a dynamical approach is also demonstrated. The meson cloud effects as well as the coupled-channels contributions to the {gamma}N{yields}N* form factors are found to be mainly in the low Q{sup 2} region. Necessary improvements to the model and future developments are discussed.

  13. The Determination of Pseudoscalar Meson Photoproduction Amplitudes from Complete Experiments

    SciTech Connect

    A. M. Sandorfi, S. Hoblit, H. Kamano, and T-S. H. Lee

    2011-10-01

    A new generation of complete experiments is currently underway with the goal of performing a high precision extraction of pseudoscalar meson photo-production amplitudes. Here we review the most general analytic form of the cross section, dependent upon the three polarization vectors of the beam, target and recoil baryon, including all single, double and triple-polarization terms involving 16 spin-dependent observables. Analytic expressions that determine the recoil baryon polarization are also presented. Different conventions are in use in the literature and we have used a numerical calculation of cross sections from Chew-Goldberger-Low-Nambu amplitudes with arbitrary spin projections to clarify apparent sign differences. As an illustration of the use of this machinery, we carry out a multipole analysis of the gammap --> K+Lambda reaction and examine the impact of recently published polarization measurements. In fitting multipoles, we use a combined Monte Carlo sampling of the amplitude space, with gradient minimization, and find a shallow chi2 valley pitted with a very large number of local minima, despite the inclusion of recent data on 8 different observables. We conclude that, while a mathematical solution to the problem of determining an amplitude free of ambiguities may require 8 observables, as has been pointed out in the literature, experiments with realistically achievable uncertainties will require a significantly larger number.

  14. Nucleon and Δ resonances in KΣ(1385) photoproduction from nucleons

    NASA Astrophysics Data System (ADS)

    Oh, Yongseok; Ko, Che Ming; Nakayama, K.

    2008-04-01

    The reaction mechanisms for KΣ(1385) photoproduction from the reaction γp→K+Σ0(1385) in the resonance energy region are investigated in a hadronic model. Both contributions from N and Δ resonances of masses around 2 GeV as given in the Review of Particle Data Group and by the quark model predictions are included. The Lagrangians for describing the decays of these resonances into KΣ(1385) are constructed with the coupling constants determined from the decay amplitudes predicted by a quark model. Comparing the resulting total cross section for the reaction γp→K+Σ0(1385) with the preliminary data from the Thomas Jefferson National Accelerator Facility, we find that the most important contributions are from the two-star-rated resonances Δ(2000)F35,Δ(1940)D33, and N(2080)D13, as well as the missing resonance N(3)/(2)-(2095) predicted in the quark model. Predictions on the differential cross section and photon asymmetry in this reaction are also given.

  15. Rescattering in Meson Photoproduction off Few Body Systems

    SciTech Connect

    Jean-Marc Laget

    2006-04-01

    Exclusive reactions induced at high momentum transfer in few body systems allow to adjust the formation time of the produced particles to the distance between two nucleons in the target. They are the best windows to study the propagation of exotic configurations of hadrons such as for instance the onset of color transparency. It may appear earlier in meson photo-production reactions, in the strange sector more particularly, than in more classical quasi elastic scattering of electrons. More generally, those reactions provide them with the best tool to determine the cross section of the scattering of various hadrons (strange particles, vector mesons) with nucleon, to better understand the mechanisms of their formation in cold hadronic matter, and to access the production of possible exotic states. At the top of the unitary rescattering peak (triangular logarithmic singularity), the reaction amplitude is on solid ground since it depends only on on-shell elementary amplitudes and on low momentum components of the nuclear wave function.

  16. Role of two-nucleon mechanisms in pion photoproduction on nuclei in the region of high momentum transfers

    SciTech Connect

    Egorov, M. V.; Fix, A. I.

    2013-05-15

    The role of two-nucleon mechanisms in pion photoproduction on nuclei was studied in the region of high momentum transfers to the residual nucleus. The process in which the photoproduction of negative pions on a {sup 12}C nucleus is accompanied by proton emission was considered by way of example. The results of the calculations were compared with available experimental data.

  17. An investigation of adhesive/adherend and fiber matrix interactions. [Ti 6-4 surfaces

    NASA Technical Reports Server (NTRS)

    Beck, B.; Siriwardane, R.; Wightman, J. P.

    1981-01-01

    Research during the report period focused on continued scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) of lap shear samples and flatwise tensile specimens and on the surface characterization of TiO2, Ti 6-4, and Ti powders with particular emphasis on their interaction with primer solutions of both polyphenylquinoxaline and LaRC-13 polyimide. The use of SEM and XPS in the analysis of Ti 6-4 adherend surfaces is described as well as differences in Ti 6-4 surface composition after different chemical pretreatments. Analysis of fractured surfaces is used to established the failure mode. The surface acidity of Ti 6-4 coupons can be established by reflectance visible spectroscopy using indicator dyes.

  18. ϕ-meson photoproduction on hydrogen in the neutral decay mode

    NASA Astrophysics Data System (ADS)

    Seraydaryan, H.; Amaryan, M. J.; Gavalian, G.; Baghdasaryan, H.; Weinstein, L.; Adhikari, K. P.; Adikaram, D.; Aghasyan, M.; Anderson, M. D.; Pereira, S. Anefalos; Avakian, H.; Ball, J.; Baltzell, N. A.; Battaglieri, M.; Batourine, V.; Bedlinskiy, I.; Bennett, R. P.; Biselli, A. S.; Bono, J.; Boiarinov, S.; Briscoe, W. J.; Brooks, W. K.; Bültmann, S.; Burkert, V. D.; Carman, D. S.; Celentano, A.; Chandavar, S.; Collins, P.; Contalbrigo, M.; Cortes, O.; Crede, V.; D'Angelo, A.; Dashyan, N.; De Vita, R.; De Sanctis, E.; Deur, A.; Djalali, C.; Doughty, D.; Dugger, M.; Dupre, R.; Fassi, L. El; Eugenio, P.; Fedotov, G.; Fegan, S.; Fersch, R.; Fleming, J. A.; Gevorgyan, N.; Giovanetti, K. L.; Girod, F. X.; Goetz, J. T.; Gohn, W.; Golovatch, E.; Gothe, R. W.; Griffioen, K. A.; Guidal, M.; Guler, N.; Guo, L.; Hafidi, K.; Hakobyan, H.; Hanretty, C.; Harrison, N.; Heddle, D.; Hicks, K.; Ho, D.; Holtrop, M.; Hyde, C. E.; Ilieva, Y.; Ireland, D. G.; Ishkhanov, B. S.; Isupov, E. L.; Jo, H. S.; Joo, K.; Keller, D.; Khandaker, M.; Kim, A.; Kim, W.; Klein, F. J.; Koirala, S.; Kubarovsky, A.; Kubarovsky, V.; Kuhn, S. E.; Kuleshov, S. V.; Lewis, S.; Livingston, K.; Lu, H. Y.; MacGregor, I. J. D.; Martinez, D.; Mayer, M.; McKinnon, B.; Mineeva, T.; Mirazita, M.; Mokeev, V.; Montgomery, R. A.; Moutarde, H.; Munevar, E.; Camacho, C. Munoz; Nadel-Turonski, P.; Nasseripour, R.; Niccolai, S.; Niculescu, I.; Osipenko, M.; Ostrovidov, A. I.; Pappalardo, L. L.; Paremuzyan, R.; Park, K.; Park, S.; Pasyuk, E.; Phelps, E.; Phillips, J. J.; Pisano, S.; Pogorelko, O.; Pozdniakov, S.; Price, J. W.; Protopopescu, D.; Puckett, A. J. R.; Rimal, D.; Ripani, M.; Ritchie, B. G.; Rizzo, A.; Rosner, G.; Rossi, P.; Sabatié, F.; Saini, M. S.; Salgado, C.; Schott, D.; Schumacher, R. A.; Seder, E.; Sharabian, Y. G.; Smith, G. D.; Sober, D. I.; Sokhan, D.; Stepanyan, S.; Stoler, P.; Strakovsky, I. I.; Strauch, S.; Tang, W.; Taylor, C. E.; Tian, Ye; Tkachenko, S.; Ungaro, M.; Vineyard, M. F.; Voskanyan, H.; Voutier, E.; Walford, N. K.; Watts, D. P.; Weinstein, L. B.; Wood, M. H.; Zachariou, N.; Zana, L.; Zhang, J.; Zhao, Z. W.; CLAS Collaboration

    2014-05-01

    We report the first measurement of the photoproduction cross section of the ϕ meson in its neutral decay mode in the reaction γp →pϕ(KSKL). The experiment was performed with a tagged photon beam of energy 1.6≤Eγ≤3.6 GeV incident on a liquid hydrogen target of the CLAS spectrometer at the Thomas Jefferson National Accelerator Facility. The pϕ final state is identified via reconstruction of KS in the invariant mass of two oppositely charged pions and by requiring the missing particle in the reaction γp →pKSX to be KL. The presented results significantly enlarge the existing data on ϕ photoproduction. These data, combined with the data from the charged decay mode, will help to constrain different mechanisms of ϕ photoproduction.

  19. Partial-wave expansion for photoproduction of two pseudoscalars on a nucleon

    NASA Astrophysics Data System (ADS)

    Fix, A.; Arenhövel, H.

    2012-03-01

    The amplitudes for photoproduction of two pseudoscalars on a nucleon are expanded in the overall center-of-momentum (c.m.) frame in a model-independent way with respect to the contribution of the final-state partial wave of total angular momentum J and its projection on the normal to the plane spanned by the momenta of the final particles. The expansion coefficients, which are analogs to the multipole amplitudes for single-meson photoproduction, contain the complete information about the reaction dynamics. Results of an explicit evaluation are presented for the moments Wjm of the inclusive angular distribution of an incident photon beam with respect to the c.m. coordinate system defined by the final particles, taking photoproduction of π0π0 and π0η as an example.

  20. Use Of A Quartz Crystal Microbalance Rate Monitor To Examine Photoproduct Effects On Resist Dissolution

    NASA Astrophysics Data System (ADS)

    Hinsberg, W. D.; Willson, C. G.; Kanazawa, K. K.

    1985-04-01

    A new general method for measuring dissolution kinetics of thin films has been developed. This technique employs a quartz crystal microbalance to measure the mass of the dissolving thin film. The method allows the measurement of very rapid dissolution rates, and can also be used to study the dissolution kinetics of thick or opaque films. The technique has several advantages over alternative in situ methods based on optical interferometry or capacitance. This instrument has been used to examine the effects of photoproducts on the dissolution kinetics of positive photoresist. The influence of photolytically generated carboxylic acid, and the nitrogen byproduct entrapped in the film, have been independently assessed by comparing the solubility of films of novolac resin, and films of resin plus carboxylic acid photoproduct, with that of exposed photoresist. Our results indicate that the acid does not significantly influence the solubility of the resin, and that entrapped gaseous photoproducts exert a rate-enhancing effect.

  1. Double and single pion photoproduction within a dynamical coupled-channels model

    SciTech Connect

    Kamano, H.; Julia-Diaz, B.; Lee, T.-S. H.; Matsuyama, A.; Sato, T.

    2009-12-15

    Within a dynamical coupled-channels model that has already been fixed by analyzing the data of the {pi}N{yields}{pi}N and {gamma}N{yields}{pi}N reactions, we present the predicted double pion photoproduction cross sections up to the second resonance region, W<1.7 GeV. The roles played by the different mechanisms within our model in determining both the single and double pion photoproduction reactions are analyzed, focusing on the effects attributable to the direct {gamma}N{yields}{pi}{pi}N mechanism, the interplay between the resonant and nonresonant amplitudes, and the coupled-channels effects. The model parameters that can be determined most effectively in the combined studies of both the single and double pion photoproduction data are identified for future studies.

  2. Double and single pion photoproduction within a dynamical coupled-channels model

    SciTech Connect

    Hiroyuki Kamano; Julia-Diaz, Bruno; Lee, T. -S. H.; Matsuyama, Akihiko; Sato, Toru

    2009-12-16

    Within a dynamical coupled-channels model which has already been fixed from analyzing the data of the πN → πN and γN → πN reactions, we present the predicted double pion photoproduction cross sections up to the second resonance region, W < 1.7 GeV. The roles played by the different mechanisms within our model in determining both the single and double pion photoproduction reactions are analyzed, focusing on the effects due to the direct γN → ππN mechanism, the interplay between the resonant and non-resonant amplitudes, and the coupled-channels effects. As a result, the model parameters which can be determined most effectively in the combined studies of both the single and double pion photoproduction data are identified for future studies.

  3. Double and single pion photoproduction within a dynamical coupled-channels model

    DOE PAGESBeta

    Hiroyuki Kamano; Julia-Diaz, Bruno; Lee, T. -S. H.; Matsuyama, Akihiko; Sato, Toru

    2009-12-16

    Within a dynamical coupled-channels model which has already been fixed from analyzing the data of the πN → πN and γN → πN reactions, we present the predicted double pion photoproduction cross sections up to the second resonance region, W < 1.7 GeV. The roles played by the different mechanisms within our model in determining both the single and double pion photoproduction reactions are analyzed, focusing on the effects due to the direct γN → ππN mechanism, the interplay between the resonant and non-resonant amplitudes, and the coupled-channels effects. As a result, the model parameters which can be determined mostmore » effectively in the combined studies of both the single and double pion photoproduction data are identified for future studies.« less

  4. phi-meson photoproduction on Hydrogen in the neutral decay mode

    SciTech Connect

    Seraydaryan, Helena; Amaryan, Moscov J.; Gavalian, Gagik; Baghdasaryan, Hovhannes A.; Weinstein, Larry

    2014-05-01

    We report the first measurement of the photoproduction cross section of the $\\phi$ meson in its neutral decay mode in the reaction $\\gamma p \\to p\\phi(K_SK_L)$. The experiment was performed with a tagged photon beam of energy $1.6 \\le E_\\gamma \\le 3.6$ GeV incident on a liquid hydrogen target of the CLAS spectrometer at the Thomas Jefferson National Accelerator Facility. The $p \\phi$ final state is identified via reconstruction of $K_S$ in the invariant mass of two oppositely charged pions and by requiring the missing particle in the reaction $\\gamma p \\to p K_S X$ to be $K_L$. The presented results significantly enlarge the existing data on $\\phi$-photoproduction. These data, combined with the data from the charged decay mode, will help to constrain different mechanisms of $\\phi$ photoproduction.

  5. Immobilized chloroplast-ferredoxin-hydrogenase system for the simultaneous photoproduction of hydrogen and oxygen

    SciTech Connect

    Woodward, J.; Greenbaum, E.

    1983-01-01

    The chloroplast-ferredoxin-hydrogenase (CFH) system is capable of the simultaneous photoproduction of hydrogen and oxygen using water as the source of electrons. The immobilization of chloroplasts and hydrogenase, which has been the subject of several reports, has been attempted in order to increase their storage and functional stability. However, immobilized chloroplasts and hydrogenase have never been shown to produce hydrogen and oxygen simultaneously. The simultaneous photoproduction of hydrogen and oxygen is a valuable guide in assessing the role of water as the primary substrate in biophotolysis. As has been shown previously, the stoichiometric ratio of hydrogen to oxygen is, in general, not equal to two. By measuring the oxygen that is produced along with the hydrogen, it is possible to set an upper limit on the photoproduced hydrogen that is derived from water. The study reported here describes for the first time the simultaneous photoproduction of hydrogen and oxygen by the CFH system immobilized in calcium alginate gel spheres.

  6. Photoproduction of neutral kaons on a liquid deuterium target in the threshold region

    SciTech Connect

    Tsukada, K.; Takahashi, T.; Watanabe, T.; Fujii, Y.; Futatsukawa, K.; Hashimoto, O.; Hirose, K.; Ito, K.; Kameoka, S.; Kanda, H.; Maeda, K.; Matsumura, A.; Miura, Y.; Miyase, H.; Nakamura, S. N.; Nomura, H.; Nonaka, K.; Osaka, T.; Okayasu, Y.; Tamura, H.

    2008-07-15

    The photoproduction process of neutral kaons on a liquid deuterium target is investigated near the threshold region, E{sub {gamma}}=0.8-1.1 GeV. K{sup 0} events are reconstructed from positive and negative pions, and differential cross sections are derived. Experimental momentum spectra are compared with those calculated in the spectator model using a realistic deuteron wave function. Elementary amplitudes as given by recent isobar models and a simple phenomenological model are used to study the effect of the new data on the angular behavior of the elementary cross section. The data favor a backward-peaked angular distribution of the elementary n({gamma},K{sup 0}){lambda} process, which provides additional constraints on current models of kaon photoproduction. The present study demonstrates that the n({gamma},K{sup 0}){lambda} reaction can provide key information on the mechanism of the photoproduction of strangeness.

  7. Sustainable Hydrogen Photoproduction by Phosphorus-Deprived Marine Green Microalgae Chlorella sp.

    PubMed Central

    Batyrova, Khorcheska; Gavrisheva, Anastasia; Ivanova, Elena; Liu, Jianguo; Tsygankov, Anatoly

    2015-01-01

    Previously it has been shown that green microalga Chlamydomonas reinhardtii is capable of prolonged H2 photoproduction when deprived of sulfur. In addition to sulfur deprivation (-S), sustained H2 photoproduction in C. reinhardtii cultures can be achieved under phosphorus-deprived (-P) conditions. Similar to sulfur deprivation, phosphorus deprivation limits O2 evolving activity in algal cells and causes other metabolic changes that are favorable for H2 photoproduction. Although significant advances in H2 photoproduction have recently been realized in fresh water microalgae, relatively few studies have focused on H2 production in marine green microalgae. In the present study phosphorus deprivation was applied for hydrogen production in marine green microalgae Chlorella sp., where sulfur deprivation is impossible due to a high concentration of sulfates in the sea water. Since resources of fresh water on earth are limited, the possibility of hydrogen production in seawater is more attractive. In order to achieve H2 photoproduction in P-deprived marine green microalgae Chlorella sp., the dilution approach was applied. Cultures diluted to about 0.5–1.8 mg Chl·L−1 in the beginning of P-deprivation were able to establish anaerobiosis, after the initial growth period, where cells utilize intracellular phosphorus, with subsequent transition to H2 photoproduction stage. It appears that marine microalgae during P-deprivation passed the same stages of adaptation as fresh water microalgae. The presence of inorganic carbon was essential for starch accumulation and subsequent hydrogen production by microalgae. The H2 accumulation was up to 40 mL H2 gas per 1iter of the culture, which is comparable to that obtained in P-deprived C. reinhardtii culture. PMID:25629229

  8. Sustainable hydrogen photoproduction by phosphorus-deprived marine green microalgae Chlorella sp.

    PubMed

    Batyrova, Khorcheska; Gavrisheva, Anastasia; Ivanova, Elena; Liu, Jianguo; Tsygankov, Anatoly

    2015-01-01

    Previously it has been shown that green microalga Chlamydomonas reinhardtii is capable of prolonged H2 photoproduction when deprived of sulfur. In addition to sulfur deprivation (-S), sustained H2 photoproduction in C. reinhardtii cultures can be achieved under phosphorus-deprived (-P) conditions. Similar to sulfur deprivation, phosphorus deprivation limits O2 evolving activity in algal cells and causes other metabolic changes that are favorable for H2 photoproduction. Although significant advances in H2 photoproduction have recently been realized in fresh water microalgae, relatively few studies have focused on H2 production in marine green microalgae. In the present study phosphorus deprivation was applied for hydrogen production in marine green microalgae Chlorella sp., where sulfur deprivation is impossible due to a high concentration of sulfates in the sea water. Since resources of fresh water on earth are limited, the possibility of hydrogen production in seawater is more attractive. In order to achieve H2 photoproduction in P-deprived marine green microalgae Chlorella sp., the dilution approach was applied. Cultures diluted to about 0.5-1.8 mg Chl·L-1 in the beginning of P-deprivation were able to establish anaerobiosis, after the initial growth period, where cells utilize intracellular phosphorus, with subsequent transition to H2 photoproduction stage. It appears that marine microalgae during P-deprivation passed the same stages of adaptation as fresh water microalgae. The presence of inorganic carbon was essential for starch accumulation and subsequent hydrogen production by microalgae. The H2 accumulation was up to 40 mL H2 gas per 1iter of the culture, which is comparable to that obtained in P-deprived C. reinhardtii culture. PMID:25629229

  9. Helicity Amplitudes for Photoproduction of Baryons with J = 1/2 and JP = 3/2+

    NASA Astrophysics Data System (ADS)

    Liu, Lei-Hua

    2015-12-01

    We derive the separate helicity amplitudes using the partial wave analysis in the process of pseudo-scalar meson photoproduction. For JP = 3/2+, we find the amplitude is model independent. According to parity conservation, the general amplitude in the case of JP = 1/2- is obtained. We prove this general amplitude corresponds to the situation of λ = -1 when adopting the circular polarization. Finally, the formulas of scattering amplitudes involving the meson photoproduction with JP = 3/2+ are obtained from the chiral quark model. Supported by the Chinese Scholarship Council (CSC)

  10. Determination of E and G Observables in n Photoproduction on the CLAS Frozen Spin Target (FROST)

    SciTech Connect

    Senderovich, Igor; Morrison, Brian T.; Dugger, Michael R.; Ritchie, Barry G.; Tucker, Ross J.

    2014-01-01

    Polarization observables are vital for disentangling overlapping resonances in the baryon spectrum. Extensive data have been collected at Jefferson Lab in Hall B with circularly and linearly polarized tagged photon beam incident on longitudinally polarized protons provided by the Frozen Spin Target (FROST). The focus of the described work is on η photoproduction, which acts as an "isospin filter", isolating the N*(I = 1/2) resonances. Preliminary results for the double-polarization observables E and G are presented. There are currently no data on these in the world database for η photoproduction.

  11. Studying the Pc(4450 ) resonance in J /ψ photoproduction off protons

    NASA Astrophysics Data System (ADS)

    Blin, A. N. Hiller; Fernández-Ramírez, C.; Jackura, A.; Mathieu, V.; Mokeev, V. I.; Pilloni, A.; Szczepaniak, A. P.; Joint Physics Analysis Center

    2016-08-01

    A resonancelike structure, the Pc(4450 ), has recently been observed in the J /ψ p spectrum by the LHCb Collaboration. We discuss the feasibility of detecting this structure in J /ψ photoproduction in the CLAS12 experiment at JLab. We present a first estimate of the upper limit for the branching ratio of the Pc(4450 ) to J /ψ p . Our estimates, which take into account the experimental resolution effects, predict that it will be possible to observe a sizable cross section close to the J /ψ production threshold and shed light on the Pc(4450 ) resonance in the future photoproduction measurements.

  12. Preliminary results for the helicity asymmetry E for eta photoproduction on the proton

    SciTech Connect

    B. T. Morrison, M. Dugger, B. G. Ritchie, CLAS Collaboration

    2012-04-01

    Polarization observables are an important tool for clarifying the nucleon resonance spectrum. No previous measurements for double polarization asymmetries have been published for eta photoproduction. Double polarization measurements have been made at Jefferson Lab using a polarized photon beam and protons in a polarized frozen spin target (FROST). Data were taken during the first running period of FROST using the CLAS detector with photon energies from 0.33 to 2.35 GeV. Preliminary results for the E polarization observable for eta meson photoproduction from the proton at threshold and above, along with comparisons to several theoretical predictions are presented.

  13. CLAS+FROST: new generation of photoproduction experiments at Jefferson Lab

    SciTech Connect

    Eugene Pasyuk

    2009-12-01

    A large part of the experimental program in Hall B of the Jefferson Lab is dedicated to baryon spectroscopy. Photoproduction experiments are essential part of this program. CEBAF Large Acceptance Spectrometer (CLAS) and availability of circularly and linearly polarized tagged photon beams provide unique conditions for this type of experiments. Recent addition of the Frozen Spin Target (FROST) gives a remarkable opportunity to measure double and triple polarization observables for different pseudo-scalar meson photoproduction processes. For the first time, a complete or nearly complete experiment becomes possible and will allow model independent extraction of the reaction amplitude. An overview of the experiment and its current status is presented.

  14. Measurement of Beauty and Charm Photoproduction at H1 using inclusive lifetime tagging

    SciTech Connect

    Finke, L.

    2005-10-06

    A measurement of the charm and beauty photoproduction cross sections at the ep collider HERA is presented. The lifetime signature of c and b-flavoured hadrons is exploited to determine the fractions of events in the sample containing charm or beauty. Differential cross sections as a function of the jet transverse momentum, the rapidity and x{sub {gamma}}{sup obs} are measured in the photoproduction region Q2 < 1 GeV2, with inelasticity 0.15 < y < 0.8. The results are compared with calculations in next-to-leading order perturbative QCD and Monte Carlo models as implemented in PYTHIA and CASCADE.

  15. Light-induced conformational change and product release in DNA repair by (6-4) photolyase

    PubMed Central

    Kondoh, Masato; Hitomi, Kenichi; Yamamoto, Junpei; Todo, Takeshi; Iwai, Shigenori; Getzoff, Elizabeth D.; Terazima, Masahide

    2012-01-01

    Proteins of the cryptochrome/photolyase family share high sequence similarities, common folds and the flavin adenine dinucleotide (FAD) cofactor, but exhibit diverse physiological functions. Mammalian cryptochromes are essential regulatory components of the 24-hour circadian clock, whereas (6-4) photolyases recognize and repair UV-induced DNA damage by using light energy absorbed by FAD. Despite increasing knowledge about physiological functions from genetic analyses, the molecular mechanisms and conformational dynamics involved in clock signaling and DNA repair remain poorly understood. The (6-4) photolyase, which has strikingly high similarity to human clock cryptochromes, is a prototypic biological system to study conformational dynamics of cryptochrome/photolyase family proteins. The entire light-dependent DNA repair process for (6-4) photolyase can be reproduced in a simple in vitro system. To decipher pivotal reactions of the common FAD cofactor, we accomplished time resolved measurements of radical formation, diffusion, and protein conformational changes during light-dependent repair by full-length (6-4) photolyase on DNA carrying a single UV-induced damage. The (6-4) photolyase by itself showed significant volume changes after blue light activation, indicating protein conformational changes distant from the flavin cofactor. A drastic diffusion change was observed only in the presence of both (6-4) photolyase and damaged DNA, and not for (6-4) photolyase alone or with undamaged DNA. Thus, we propose that this diffusion change reflects the rapid (50 μs time constant) dissociation of the protein from the repaired DNA product. Conformational changes with such fast turnover would likely enable DNA repair photolyases to access the entire genome in cells. PMID:21271694

  16. Study of the Two-pion Photoproduction on the Deuteron

    SciTech Connect

    Graham, Lewis P.

    2012-12-01

    Understanding the structure of baryons in terms of the fundamental interaction of the constituent quarks and gluons is one of the primary challenges in strong interaction physics. This interaction is governed by Quantum Chromodynamics (QCD), which is a theory for understanding the dynamics of strong. QCD displays the asymptotic freedom of hadrons at very short distances and also the confinement of quarks and gluons inside hadrons. However, solutions of this QCD theory in the non-perturbative domain of the interaction are extremely difficult to achieve, mainly because confinement happens on the hadronic scale on which the coupling constant is large and prevents any perturbative approach. Thus leaving us with strategies such as lattice QCD or formulating QCD sum rules to get around this problem. In exclusive hadron production the yN interaction is recognized for being a powerful method for investigating hadrons and the mysteries that still exist within the strong interaction. From reactions with the nucleon, the strong interaction can be investigated through the transition amplitudes to the N and Delta resonances. More specifically, if an electromagnetic interaction is well known then the intermediate resonance states may be evaluated through meson photoproduction. To gain more detailed insight into this interaction, we look to probe the baryon structure of the nucleon and the photo-excited resonance decays through photon scattering off a deuteron producing two pions in the final state. This photoproduction process off the deuteron will be used to investigate known baryon resonances in the two pion channel. The two pion final state will be investigated for unraveling new information into the photo-coupling strengths. We want to explore final state interactions, search for properties of known resonances, and to explore the possibility of seeing missing states that are predicted by quark models but have not yet been found experimentally. Using the CEBAF Large

  17. Photoproduction of π0-pairs off protons and off neutrons

    NASA Astrophysics Data System (ADS)

    Dieterle, M.; Oberle, M.; Ahrens, J.; Annand, J. R. M.; Arends, H. J.; Bantawa, K.; Bartolome, P. A.; Beck, R.; Bekrenev, V.; Berghäuser, H.; Braghieri, A.; Branford, D.; Briscoe, W. J.; Brudvik, J.; Cherepnya, S.; Costanza, S.; Demissie, B.; Downie, E. J.; Drexler, P.; Fil'kov, L. V.; Fix, A.; Garni, S.; Glazier, D. I.; Hamilton, D.; Heid, E.; Hornidge, D.; Howdle, D.; Huber, G. M.; Jahn, O.; Jude, T. C.; Käser, A.; Kashevarov, V. L.; Keshelashvili, I.; Kondratiev, R.; Korolija, M.; Krusche, B.; Lisin, V.; Livingston, K.; MacGregor, I. J. D.; Maghrbi, Y.; Mancell, J.; Manley, D. M.; Marinides, Z.; McGeorge, J. C.; McNicoll, E.; Mekterovic, D.; Metag, V.; Micanovic, S.; Middleton, D. G.; Mushkarenkov, A.; Nikolaev, A.; Novotny, R.; Ostrick, M.; Otte, P.; Oussena, B.; Pedroni, P.; Pheron, F.; Polonski, A.; Prakhov, S.; Robinson, J.; Rostomyan, T.; Schumann, S.; Sikora, M. H.; Sober, D. I.; Starostin, A.; Strub, Th.; Supek, I.; Thiel, M.; Thomas, A.; Unverzagt, M.; Walford, N. K.; Watts, D. P.; Werthmüller, D.; Witthauer, L.

    2015-11-01

    Total cross sections, angular distributions, and invariant-mass distributions have been measured for the photoproduction of π0π0 pairs off free protons and off nucleons bound in the deuteron. The experiments were performed at the MAMI accelerator facility in Mainz using the Glasgow photon tagging spectrometer and the Crystal Ball/TAPS detector. The accelerator delivered electron beams of 1508 and 1557MeV, which produced bremsstrahlung in thin radiator foils. The tagged photon beam covered energies up to 1400MeV. The data from the free proton target are in good agreement with previous measurements and were only used to test the analysis procedures. The results for differential cross sections (angular distributions and invariant-mass distributions) for free and quasi-free protons are almost identical in shape, but differ in absolute magnitude up to 15%. Thus, moderate final-state interaction effects are present. The data for quasi-free neutrons are similar to the proton data in the second resonance region (final-state invariant masses up to ≈ 1550 MeV), where both reactions are dominated by the N(1520)3/2- → Δ(1232)3/2+π decay. At higher energies, angular and invariant-mass distributions are different. A simple analysis of the shapes of the invariant-mass distributions in the third resonance region is consistent with strong contributions of an N^{star}→ Nσ decay for the proton, while the reaction is dominated by a sequential decay via a Δπ intermediate state for the neutron. The data are compared to predictions from the Two-Pion-MAID model and the Bonn-Gatchina coupled-channel analysis.

  18. Photoproduction of η π pairs off nucleons and deuterons

    NASA Astrophysics Data System (ADS)

    Käser, A.; Müller, F.; Ahrens, J.; Annand, J. R. M.; Arends, H. J.; Bantawa, K.; Bartolome, P. A.; Beck, R.; Braghieri, A.; Briscoe, W. J.; Cherepnya, S.; Costanza, S.; Dieterle, M.; Downie, E. J.; Drexler, P.; Fil'kov, L. V.; Fix, A.; Garni, S.; Glazier, D. I.; Hamilton, D.; Hornidge, D.; Howdle, D.; Huber, G. M.; Jaegle, I.; Jude, T. C.; Kashevarov, V. L.; Keshelashvili, I.; Kondratiev, R.; Korolija, M.; Krusche, B.; Lisin, V.; Livingston, K.; MacGregor, I. J. D.; Maghrbi, Y.; Mancell, J.; Manley, D. M.; Marinides, Z.; McGeorge, J. C.; McNicoll, E.; Mekterovic, D.; Metag, V.; Micanovic, S.; Middleton, D. G.; Mushkarenkov, A.; Nikolaev, A.; Novotny, R.; Oberle, M.; Ostrick, M.; Otte, P.; Oussena, B.; Pedroni, P.; Pheron, F.; Polonski, A.; Prakhov, S.; Robinson, J.; Rostomyan, T.; Schumann, S.; Sikora, M. H.; Sober, D.; Starostin, A.; Strub, Th.; Supek, I.; Thiel, M.; Thomas, A.; Unverzagt, M.; Walford, N. K.; Watts, D. P.; Werthmüller, D.; Witthauer, L.

    2016-09-01

    Quasi-free photoproduction of πη-pairs has been investigated from threshold up to incident photon energies of 1.4 GeV, respectively up to photon-nucleon invariant masses up to 1.9 GeV. Total cross sections, angular distributions, invariant-mass distributions of the πη and meson-nucleon pairs, and beam-helicity asymmetries have been measured for the reactions γ p→ pπ0η, γ n→ nπ0η , γ p→ nπ+η , and γ n→ pπ-η from nucleons bound inside the deuteron. For the γ p initial-state data for free protons have also been analyzed. Finally, the total cross sections for quasi-free production of π0η pairs from nucleons bound in 3 He nuclei have been investigated in view of final state interaction (FSI) effects. The experiments were performed at the tagged photon beam facility of the Mainz MAMI accelerator using an almost 4 π covering electromagnetic calorimeter composed of the Crystal Ball and TAPS detectors. The shapes of all differential cross section data and the asymmetries are very similar for protons and neutrons and agree with the conjecture that the reactions are dominated by the sequential Δ^{star}3/2-→ ηΔ(1232) →πη N decay chain, mainly with Δ(1700)3/2- and Δ(1940)3/2- . The ratios of the magnitude of the total cross sections also agree with this assumption. However, the absolute magnitudes of the cross sections are reduced by FSI effects with respect to free proton data.

  19. Role of Lys281 in the Dunaliella salina (6-4) photolyase reaction.

    PubMed

    Zhang, Feiwei; Xu, Hui; Cao, Yu; Wen, Tao; Lin, Jiafu; Ma, Gen; Qiao, Dairong; Cao, Yi

    2011-01-01

    His(354) and His(358), two highly conserved histidines in Xenopus laevis (6-4) photolyase [equivalent to His(401) and His(405), in Dunaliella salina (6-4) photolyase], are critical for photoreactivation. They act as a base and an acid, respectively. However, the remaining high repair activity when the pH value is higher than the pKa of histidine suggests the involvement of other basic amino acids in photoreactivation. According to the results of in vivo enzyme assay and three-dimension structural model of Dunaliella salina (6-4) photolyase we hypothesized that Lys(281) might be involved in the photoreactivation over the pH range from 10.0 to 11.0. To test this, we generated two mutant forms of the (6-4) photolyase, K281G and K281R mutant, by overlap extension polymerase chain reaction, and performed the enzyme assay with these mutants. From these results we conclude that the Lys(281), which is highly conserved in (6-4) photolyases, participates in the photoreactivation and acts as an acid to donate a proton to His(401) when the environmental pH is higher than the pKa value of histidine.

  20. Eta photoproduction in a combined analysis of pion- and photon-induced reactions

    SciTech Connect

    Ronchen, D.; Doring, M.; Haberzettl, H.; Haidenbauer, J.; MeiBner, U. -G.; Nakayama, K.

    2015-06-25

    The $\\eta N$ final state is isospin-selective and thus provides access to the spectrum of excited nucleons without being affected by excited $\\Delta$ states. To this end, the world database on eta photoproduction off the proton up to a center-of-mass energy of $E\\sim 2.3$ GeV is analyzed, including data on differential cross sections, and single and double polarization observables. The resonance spectrum and its properties are determined in a combined analysis of eta and pion photoproduction off the proton together with the reactions $\\pi N\\to \\pi N$, $\\eta N$, $K\\Lambda$ and $K\\Sigma$. For the analysis, the so-called J\\"ulich coupled-channel framework is used, incorporating unitarity, analyticity, and effective three-body channels. Parameters tied to photoproduction and hadronic interactions are varied simultaneously. Furthermore, the influence of recent MAMI $T$ and $F$ asymmetry data on the eta photoproduction amplitude is discussed in detail.

  1. Survey of selected seaweeds for simultaneous photoproduction of hydrogen and oxygen

    SciTech Connect

    Greenbaum, E.; Ramus, J.

    1983-03-01

    Then seaweed species were surveyed for simultaneous photoevolution of hydrogen and oxygen. In an attempt to induce hydrogenase activity (as measured by hydrogen photoproduction) the seaweeds were maintained under anaerobiosis in CO/sub 2/-free seawater for varying lengths of time. Although oxygen evolution was observed in every alga studied, hydrogen evolution was not observed. One conclusion of this research is that, in contrast to the microscopic algae, there is not a single example of a macroscopic alga for which the photoevolution of hydrogen has been observed, in spite of the fact that there are now at least nine macroscopic algal species known for which hydrogenase activity has been reported (either by dark hydrogen evolution or light-activated hydrogen uptake). These results are in conflict with the conventional view that algal hydrogenase can catalyze a multiplicity of reactions, one of which is the photoproduction of molecular hydrogen. Two possible explanations for the lack of hydrogen photoproduction in macroscopic algae are presented. It is postulated that electron acceptors other than carbon dioxide can take up reducing equivalents from Photosystem I to the measurable exclusion of hydrogen photoproduction. Alternatively, the hydrogenase system in macroscopic algae may be primarily a hydrogen-uptake system with respect to light-activated reactions. A simple kinetic argument based on recent measurements of the photosynthetic turnover times of simultaneous light-activated hydrogen and oxygen production is presented that supports the second explanation. 25 references, 3 figures, 1 table.

  2. Identification of UV photoproducts and hydrolysis products of butachlor by mass spectrometry.

    PubMed

    Zheng, H H; Ye, C M

    2001-07-15

    The photoproducts and hydrolysis products of butachlor in water were identified by gas chromatography/mass spectrometry. When exposed to UV light, butachlor in aqueous solution was rapidly degraded, giving at least 11 photoproducts as a result of dechlorination with subsequent hydroxylation or cyclization processes. The chemical structures of nine degradation compounds were identified on the basis of mass spectrum interpretation and literature data. Major photoproducts are identified as 8-ethyl-1-butoxymethyl-4-methyl-2-oxo-1,2,3,4-tetrahydro-quinoline, 2-hydroxy-2',6'-diethyl-N-(butoxymethyl) acetanilide, and a compound related to butachlor. Minor photoproducts are identified as 2,6-diethylaniline; 1-acetyl-7-ethylindole; N-(2,6-diethylphenyl)-N-(butoxymethyl)acetamide; 2-oxo-N-(2,6-diethyl-phenyl)-N-(butoxymethyl)acetamide; 1-hydroxyacetyl-2-butoxyl-3-methyl-7-ethylindole; 1-acetyl-2-butoxyl-3-methyl-7-ethylindole; and two compounds with the chemical structure unknown. The half-lives of butachlor UV photolysis were 7.54, 10.56, and 12.22 min in deionized water, river water, and paddy water, respectively. The half-lives of butachlor hydrolysis at pH 4, 7, and 10 were 630, 1155, and 1155 days at 25 +/- 1 degrees C, respectively. A hydrolysis product at pH 4 was identified by GC/MS to be 2-hydroxy-2',6'-diethyl-N-(butoxymethyl) acetanilide.

  3. Beam asymmetry {Sigma} measurements of {pi}{sup -} photoproduction on neutrons

    SciTech Connect

    Mandaglio, G.; Manganaro, M.; Giardina, G.; Mammoliti, F.; Bellini, V.; Giusa, A.; Randieri, C.; Russo, G.; Sperduto, M. L.; Bocquet, J. P.; Lleres, A.; Rebreyend, D.; D'Angelo, A.; Fantini, A.; Franco, D.; Schaerf, C.; Vegna, V.

    2010-10-15

    The -beam asymmetry {Sigma} in the photoproduction of negative pions on quasi-free neutrons in a deuterium target was measured at the Grenoble Anneau Accelerateur Laser in the energy interval 700-1500 MeV and over a wide angular range, using polarized and tagged photons. Results are compared with recent partial-wave analyses.

  4. Abiotic ammonification and gross ammonium photoproduction in the upwelling system off central Chile (36° S)

    NASA Astrophysics Data System (ADS)

    Rain-Franco, A.; Muñoz, C.; Fernandez, C.

    2012-12-01

    We investigated the production of ammonium via photodegradation of dissolved organic matter (DOM) in the coastal upwelling system off central Chile (36° S). Photoammonification experiments were carried out using exudates obtained from representative diatom species (Chaetoceros muelleri and Thalassiosira minuscule) and natural marine DOM under simulated solar radiation conditions. Additionally, we evaluated the use of photoproduced ammonium by natural microbial communities and separated ammonium oxidizing archaea and bacteria by using GC-7 as an inhibitor of the archaeal community. We found photoammonification operating at two levels: via the transformation of DOM by UV radiation (abiotic ammonification) and via the simultaneous occurrence of abiotic phototransformation and biological remineralization of DOM into NH4+ (referred as gross photoproduction of NH4+). The maximum rates of abiotic ammonification reached 0.057 μmol L-1 h-1, whereas maximum rates of gross photoproduction reached 0.746 μmol L-1 h-1. Our results also suggest that ammonium oxidizing archaea could dominate the biotic remineralization induced by photodegradation of organic matter and consequently play an important role in the local N cycle. Abiotic ammonium photoproduction in coastal upwelling systems could support between 7 and 50% of the spring-summer phytoplankton NH4+ demand. Surprisingly, gross ammonium photoproduction (remineralization induced by abiotic ammonification) might support 50 to 180% of spring-summer phytoplankton NH4+ assimilation.

  5. Helicity Asymmetry Measurement for pi0 Photoproduction with FROST

    SciTech Connect

    Iwamoto, Hideko

    2011-08-15

    This thesis reports on the first helicity asymmetry measurement for single neutral pion photoproduction using the CLAS detector in Hall B at the Thomas Jefferson National Accelerator Facility (JLab). This measurement used longitudinally polarized protons and circularly polarized photons at energies between 350 MeV and 2400 MeV. The experimental results are compared to three available model calculations.

  6. Eta photoproduction in a combined analysis of pion- and photon-induced reactions

    DOE PAGESBeta

    Ronchen, D.; Doring, M.; Haberzettl, H.; Haidenbauer, J.; MeiBner, U. -G.; Nakayama, K.

    2015-06-25

    Themore » $$\\eta N$$ final state is isospin-selective and thus provides access to the spectrum of excited nucleons without being affected by excited $$\\Delta$$ states. To this end, the world database on eta photoproduction off the proton up to a center-of-mass energy of $$E\\sim 2.3$$ GeV is analyzed, including data on differential cross sections, and single and double polarization observables. resonance spectrum and its properties are determined in a combined analysis of eta and pion photoproduction off the proton together with the reactions $$\\pi N\\to \\pi N$$, $$\\eta N$$, $$K\\Lambda$$ and $$K\\Sigma$$. For the analysis, the so-called J\\"ulich coupled-channel framework is used, incorporating unitarity, analyticity, and effective three-body channels. Parameters tied to photoproduction and hadronic interactions are varied simultaneously. Furthermore, the influence of recent MAMI $T$ and $F$ asymmetry data on the eta photoproduction amplitude is discussed in detail.« less

  7. Spectrally resolved efficiencies of carbon monoxide (CO) photoproduction in the western Canadian Arctic: particles versus solutes

    NASA Astrophysics Data System (ADS)

    Song, G.; Xie, H.; Bélanger, S.; Leymarie, E.; Babin, M.

    2013-06-01

    Spectrally resolved efficiency (i.e. apparent quantum yield, AQY) of carbon monoxide (CO) photoproduction is a useful indicator of substrate photoreactivity and a crucial parameter for modeling CO photoproduction rates in the water column. Recent evidence has suggested that CO photoproduction from particles in marine waters is significant compared to the well-known CO production from chromophoric dissolved organic matter (CDOM) photodegradation. Although CDOM-based CO AQY spectra have been extensively determined, little is known of this information on the particulate phase. Using water samples collected from the Mackenzie estuary, shelf, and Canada Basin in the southeastern Beaufort Sea, the present study for the first time quantified the AQY spectra of particle-based CO photoproduction and compared them with the concomitantly determined CDOM-based CO AQY spectra. CO AQYs of both particles and CDOM decreased with wavelength but the spectral shape of the particulate AQY was flatter in the visible regime. This feature resulted in a disproportionally higher visible light-driven CO production by particles, thereby increasing the ratio of particle- to CDOM-based CO photoproduction with depth in the euphotic zone. In terms of depth-integrated production in the euphotic zone, CO formation from CDOM was dominated by the ultraviolet (UV, 290-400 nm) radiation whereas UV and visible light played roughly equal roles in CO production from particles. Spatially, CO AQY of bulk particulate matter (i.e. the sum of organics and inorganics) augmented from the estuary and shelf to the basin while CO AQY of CDOM trended inversely. Water from the deep chlorophyll maximum layer revealed higher CO AQYs than did surface water for both particles and CDOM. CO AQY of bulk particulate matter exceeded that of CDOM on the shelf and in the basin, but the sequence reversed in the estuary. Without consideration of the potential role of metal oxides (e.g. iron oxides) in particle photochemistry

  8. Spectrally resolved efficiencies of carbon monoxide (CO) photoproduction in the Western Canadian Arctic: particles versus solutes

    NASA Astrophysics Data System (ADS)

    Song, G.; Xie, H.; Bélanger, S.; Babin, M.

    2012-11-01

    Spectrally resolved efficiency (i.e. apparent quantum yield, AQY) of carbon monoxide (CO) photoproduction is a useful indicator of substrate photoreactivity and a crucial parameter for modeling CO photoproduction rates in the water column. Recent evidence has suggested that CO photoproduction from particles in marine waters is significant compared to the well-known CO production from chromophoric dissolved organic matter (CDOM) photodegradation. Although CDOM-based CO AQY spectra have been extensively determined, little is known of this information on the particulate phase. Using water samples collected from the Mackenzie estuary, shelf, and Canada Basin in the Southeastern Beaufort Sea, the present study for the first time quantified the AQY spectra of particle-based CO photoproduction and compared them with the concomitantly determined CDOM-based CO AQY spectra. CO AQYs of both particles and CDOM decreased with wavelength but the spectral shape of the particulate AQY was flatter in the visible regime. This feature resulted in a disproportionally higher visible light-driven CO production by particles, thereby increasing the ratio of particle- to CDOM-based CO photoproduction with depth in the euphotic zone. In terms of depth-integrated production in the euphotic zone, CO formation from CDOM was dominated by the ultraviolet (UV, 290-400 nm) radiation whereas UV and visible light played roughly equal roles in CO production from particles. Spatially, CO AQY of bulk particulate matter (i.e. the sum of organics and inorganics) augmented from the estuary to shelf to basin while CO AQY of CDOM trended inversely. Water from the deep chlorophyll maximum layer revealed higher CO AQYs than did surface water for both particles and CDOM. CO AQY of bulk particulate matter exceeded that of CDOM on the shelf and in the basin but the sequence reversed in the estuary. Mineral absorption-corrected CO AQY of particulate organic matter (POM) was, however, greater than its CDOM

  9. Sustained hydrogen photoproduction by Chlamydomonas reinhardtii: Effects of culture parameters.

    PubMed

    Kosourov, Sergey; Tsygankov, Anatoly; Seibert, Michael; Ghirardi, Maria L

    2002-06-30

    The green alga, Chlamydomonas reinhardtii, is capable of sustained H(2) photoproduction when grown under sulfur-deprived conditions. This phenomenon is a result of the partial deactivation of photosynthetic O(2)-evolution activity in response to sulfur deprivation. At these reduced rates of water-oxidation, oxidative respiration under continuous illumination can establish an anaerobic environment in the culture. After 10-15 hours of anaerobiosis, sulfur-deprived algal cells induce a reversible hydrogenase and start to evolve H(2) gas in the light. Using a computer-monitored photobioreactor system, we investigated the behavior of sulfur-deprived algae and found that: (1) the cultures transition through five consecutive phases: an aerobic phase, an O(2)-consumption phase, an anaerobic phase, a H(2)-production phase and a termination phase; (2) synchronization of cell division during pre-growth with 14:10 h light:dark cycles leads to earlier establishment of anaerobiosis in the cultures and to earlier onset of the H(2)-production phase; (3) re-addition of small quantities of sulfate (12.5-50 microM MgSO(4), final concentration) to either synchronized or unsynchronized cell suspensions results in an initial increase in culture density, a higher initial specific rate of H(2) production, an increase in the length of the H(2)-production phase, and an increase in the total amount of H(2) produced; and (4) increases in the culture optical density in the presence of 50 microM sulfate result in a decrease in the initial specific rates of H(2) production and in an earlier start of the H(2)-production phase with unsynchronized cells. We suggest that the effects of sulfur re-addition on H(2) production, up to an optimal concentration, are due to an increase in the residual water-oxidation activity of the algal cells. We also demonstrate that, in principle, cells synchronized by growth under light:dark cycles can be used in an outdoor H(2)-production system without loss of

  10. The isolation and characterisation of a new type of dimeric adenine photoproduct in UV-irradiated deoxyadenylates.

    PubMed Central

    Kumar, S; Sharma, N D; Davies, R J; Phillipson, D W; McCloskey, J A

    1987-01-01

    A new type of dimeric adenine photoproduct has been isolated from d(ApA) irradiated at 254 nm in neutral aqueous solution. It is formed in comparable amounts to another, quite distinct, adenine photoproduct first described by Pörschke (J. Am. Chem. Soc. (1973), 95, 8440-8446). Results from high resolution mass spectrometry and 1H NMR indicate that the new photoproduct comprises a mixture of two stereoisomers whose formation involves covalent coupling of the adenine bases in d(ApA) and concomitant incorporation of the elements of one molecule of water. The photoproduct is degraded specifically by acid to 4,6-diamino-5-guanidinopyrimidine (DGPY) whose identity has been confirmed by independent chemical synthesis. Formation of the new photoproduct in UV-irradiated d(pA)2 and poly(dA), but not poly(rA), has been demonstrated by assaying their acid hydrolysates for the presence of DGPY. The properties of the photoproduct are consistent with it being generated by the hydrolytic fission of an azetidine photoadduct in which the N(7) and C(8) atoms of the 5'-adenine in d(ApA) are linked respectively to the C(6) and C(5) positions of the 3'-adenine. PMID:3822822

  11. Photoaffinity labeling of diphtheria toxin fragment A with NAD: structure of the photoproduct at position 148.

    PubMed

    Carroll, S F; McCloskey, J A; Crain, P F; Oppenheimer, N J; Marschner, T M; Collier, R J

    1985-11-01

    Irradiation of mixtures of diphtheria toxin fragment A and [carbonyl-14C]NAD with UV light (253.7 nm) is known to induce efficient transfer of the radiolabel to position 148, corresponding to glutamic acid in the unmodified protein. Here we report the structure of the photoproduct at position 148, as determined by chemical and photochemical methods, fast-atom-bombardment mass spectrometry, and nuclear magnetic resonance. The photoproduct [an alpha-amino-gamma-(6-nicotin-amidyl)butyric acid residue] contains the entire nicotinamide moiety of NAD linked via its number 6 carbon to the decarboxylated gamma-methylene carbon of Glu-148. No portion of the ADP-ribosyl group of NAD is present. These findings are consistent with the idea that Glu-148 lies at or near the catalytic center of diphtheria toxin. PMID:3864158

  12. Photoproduction of the rho^0 Meson on the Proton at Large Momentum Transfer

    SciTech Connect

    M. Battaglieri; E. Anciant; M. Anghinolfi; R. De Vita; E. Golovach; J. M. Laget; V. Mokeev; M. Ripani; G. Adams; M. J. Amaryan; D. S. Armstrong; B. Asavapibhop; G. Asryan; G. Audit; T. Auger; H. Avakian; S. Barrow; K. Beard; M. Bektasoglu; B. L. Berman; N. Bianchi; A. S. Biselli; S. Boiarinov; D. Branford; W. J. Briscoe; W. K. Brooks; V. D. Burkert; J. R. Calarco; G. P. Capitani; D. S. Carman; B. Carnahan; A. Cazes; C. Cetina; P. L. Cole; A. Coleman; D. Cords; P. Corvisiero; D. Crabb; H. Crannell; J. P. Cummings; E. DeSanctis; P. V. Degtyarenko; R. Demirchyan; H. Denizli; L. Dennis; K. V. Dharmawardane; K. S. Dhuga; C. Djalali; G. E. Dodge; D. Doughty; P. Dragovitsch; M. Dugger; S. Dytman; M. Eckhause; H. Egiyan; K. S. Egiyan; L. Elouadrhiri; L. Farhi; R. J. Feuerbach; J. Ficenec; T. A. Forest; A. P. Freyberger; V. Frolov; H. Funsten; S. J. Gaff; M. Gai; S. Gilad; G. P. Gilfoyle; K. L. Giovanetti; K. Griffioen; M. Guidal; M. Guillo; V. Gyurjyan; D. Hancock; J. Hardie; D. Heddle; F. W. Hersman; K. Hicks; R. S. Hicks; M. Holtrop; C. E. Hyde-Wright; M. M. Ito; K. Joo; J. H. Kelley; M. Khandaker; W. Kim; A. Klein; F. J. Klein; M. Klusman; M. Kossov; L. H. Kramer; Y. Kuang; S. E. Kuhn; D. Lawrence; M. Lucas; K. Lukashin; R. W. Major; J. J. Manak; C. Marchand; S. McAleer; J. McCarthy; J. W. C. McNabb; B. A. Mecking; M. D. Mestayer; C. A. Meyer; K. Mikhailov; R. Minehart; M. Mirazita; R. Miskimen; V. Muccifora; J. Mueller; G. S. Mutchler; J. Napolitano; S. O. Nelson; B. B. Niczyporuk; R. A. Niyazov; J. T. O'Brien; A. K. Opper; G. Peterson; S. A. Philips; N. Pivnyuk; D. Pocanic; O. Pogorelko; E. Polli; B. M. Preedom; J. W. Price; D. Protopopescu; L. M. Qin; B. A. Raue; A. R. Reolon; G. Riccardi; G. Ricco; B. G. Ritchie; F. Ronchetti; P. Rossi; D. Rowntree; P. D. Rubin; K. Sabourov; C. Salgado; M. Sanzone-Arenhovel; V. Sapunenko; R. A. Schumacher; V. S. Serov; A. Shafi; Y. G. Sharabian; J. Shaw; A. V. Skabelin; E. S. Smith; T. Smith; L. C. Smith; D. I. Sober; M. Spraker; A. Stavinsky; S. Stepanyan; P. Stoler; M. Taiuti; S. Taylor; D. J. Tedeschi; L. Todor; R. Thompson; M. F. Vineyard; A. V. Vlassov; L. B. Weinstein; A. Weisberg; H. Weller; D. P. Weygand; C. S. Whisnant; E. Wolin; M. Wood; A. Yegneswaran; J. Yun; B. Zhang; J. Zhao; Z. Zhou

    2001-10-01

    The differential cross section, d{sigma}/dt, for p0 meson photoproduction on the proton above the resonance region was measured up to a momentum transfer -t = 5 GeV2 using the CLAS detector at the Thomas Jefferson National Accelerator Facility. The p0 channel was extracted from the measured two charged-pion cross sections by fitting the {pi}+{pi}- and p{pi}+ invariant masses. The low momentum transfer region shows the typical diffractive pattern expected from Reggeon exchange. The flatter behavior at large -t cannot be explained solely in terms of QCD-inspired two-gluon exchange models. The data indicate that other processes, like quark interchange, are important to fully describe p photoproduction.

  13. Photolytic degradation of benorylate: effects of the photoproducts on cultured hepatocytes

    SciTech Connect

    Castell, J.V.; Gomez, M.J.; Mirabet, V.; Miranda, M.A.; Morera, I.M.

    1987-05-01

    The photodegradation of benorylate (4'-(acetamido)phenyl-2-acetoxybenzoate), a drug frequently used in rheumatoid arthritis therapy, has been examined under different sets of experimental conditions. Several photoproducts have been isolated and identified on the basis of their IR, NMR, and MS spectra. The most significant photochemical process is the photo-Fries rearrangement of benorylate, leading to 5-acetamido-2'-acetoxy-2-hydroxybenzophenone (1). This compound undergoes a rapid transacylation to the isomeric 5'-acetamido-2'-acetoxy-2-hydroxybenzophenone (2). A primary culture of rat hepatocytes has been used to evaluate the possible toxicity of these two benzophenones, keeping in mind the following criteria: leakage of cytosolic enzymes, attachment index to culture plates, gluconeogenesis from lactate and fructose, glycogen balance, and albumin synthesis. At the concentrations assayed, neither of the two major photoproducts of benorylate (benzophenones 1 and 2) had significant toxic effects on liver cells in culture.

  14. Study of photoproducts of Rhodamine 6G in ethanol upon powerful laser pumping

    SciTech Connect

    Batishche, S.A.; Malevich, N.A.; Mostovnikov, V.A.

    1995-04-01

    Absorption spectra of rhodamine 6G in ethanol solution are measured using, the technique of laser probing upon pumping by a doubled Nd {sup 3+}:YAG laser with pulse length{tau}{sub 01}{approx_equal}16ns. It is shown that, at the pumping energy density {ge}1.5 J/cm{sup 2}, short-lived ({tau} < 25 ns) and long-lived photoproducts formed in the dye solution, which absorbed in a wide spectral range, including the lasing region. The estimates show that the probability of rhodamine 6G transformation to the photoproduct upon three-step excitation at 532 nm achieves {approximately}2.5 X 10{sup -3}. It is noted that, in order to obtain reliable spectroscopic information using this technique, one should take into account the intense scattering of probing radiation by thermal noise gratings, which are formed due to self-diffraction of the pumping radiation into noise components.

  15. Some New Features in the Pseudoscalar Meson and Vector Meson Photoproductions

    NASA Astrophysics Data System (ADS)

    Yu, Byung Geel; Park, Yong Jae; Choi, Ki-Seok; Nam, Seung-il; Choi, Tae-Keun; Oh, Yongseok

    2013-08-01

    We investigate the role of the t-channel meson exchange in various photoproduction processes to discuss features of the respective production mechanism. For the less model-dependent analysis we work with the t-channel meson pole reggeized in the Born approximation amplitude. With the meson-baryon coupling constants chosen consistently with symmetry prediction we show that the Reggeized pole model could reproduce the experimental data to a good degree in the lower energy region. Numerical consequences show the significance of the tensor meson exchange in the γ p → K +Λ, the dominance of the pseudoscalar meson exchange in the πΔ (and KΣ*) processes, and the sizable role of the vector-meson magnetic moment in the charged ρ (and K*) photoproductions, respectively. These new features from the present analyses could provide a useful guide for future study of the N* resonances in the low energy region.

  16. Upper limits for the photoproduction cross section for the Phi--(1860) pentaquark state off the deuteron

    SciTech Connect

    Hovanes Egiyan

    2012-01-01

    We searched for the {Phi}{sup --}(1860) pentaquark in the photoproduction process off the deuteron in the {Xi}{sup -} {pi}{sup -} decay channel using CLAS. The invariant mass spectrum of the {Xi}{sup -} {pi}{sup -} system does not indicate any statistically significant enhancement near the reported mass M = 1.860 GeV. The statistical analysis of the sideband-subtracted mass spectrum yields a 90% confidence level upper limit of 0.7 nb for the photoproduction cross section of {Phi}{sup --}(1860) with a consecutive decay into {Xi}{sup -} {pi}{sup -} in the photon energy range 4.5 GeV < E{sub {gamma}} < 5.5 GeV.

  17. Chiral effective-field theory in the Delta(1232) region : II. radiative pion photoproduction

    SciTech Connect

    Vladimir Pascalutsa; Marc Vanderhaeghen

    2007-10-12

    We present a theoretical study of the radiative pion photoproduction on the nucleon ($\\gamma N \\rightarrow \\pi N \\gamma'$) in the $\\De$-resonance region, with the aim to determine the magnetic dipole moment (MDM) of the $\\Delta^+(1232)$. The study is done within the framework of chiral effective-field theory where the expansion is performed (to next-to-leading order) in the $\\delta$ power-counting scheme which is an extension of chiral perturbation theory to the $\\Delta$-resonance energy region. We present in detail the results for the absorptive part of the $\\Delta$ MDM, as well as a sensitivity study for the radiative pion photoproduction observables on the real part of the $\\Delta$ MDM. We find that an asymmetry for circular polarization of the photon beam may provide a model-independent way to measure the $\\Delta$ MDM.

  18. Weak and electromagnetic mechanisms of neutrino-pair photoproduction in a strongly magnetized electron gas

    SciTech Connect

    Borisov, A. V.; Kerimov, B. K.; Sizin, P. E.

    2012-11-15

    Expressions for the power of neutrino radiation from a degenerate electron gas in a strong magnetic field are derived for the case of neutrino-pair photoproduction via the weak and electromagnetic interaction mechanisms (it is assumed that the neutrino possesses electromagnetic form factors). It is shown that the neutrino luminosity of a medium in the electromagnetic reaction channel may exceed substantially the luminosity in the weak channel. Relative upper bounds on the effective neutrino magnetic moment are obtained.

  19. Prolongation of H2 Photoproduction by Immobilized, Sulfur-Limited Chlamydomonas reinhardtii Cultures

    SciTech Connect

    Laurinavichene, T. V.; Kosourov, S. N.; Ghirardi, M. L.; Seibert, M.; Tsygankov, A. A.

    2008-04-30

    Two approaches to prolong the duration of hydrogen production by immobilized, sulfur-limited Chlamydomonas reinhardtii cells are examined. The results demonstrate that continuous H{sub 2} photoproduction can occur for at least 90 days under constant flow of TAP medium containing micromolar sulfate concentrations. Furthermore, it is also possible to prolong the duration of H{sub 2} production by cycling immobilized cells between minus and plus sulfate conditions.

  20. Analysis of negative parity baryon photoproduction helicity amplitudes in the 1/Nc expansion

    SciTech Connect

    Jose Goity; Nicolas Matagne; Norberto Scoccola

    2007-11-26

    We study the photoproduction helicity amplitudes of negative parity baryons in the context of the 1/Nc expansion of QCD. A complete analysis to next-to-leading order is carried out. The results show sub-leading effects to be within the magnitude expected from the $1/N_c$ power counting. They also show significant deviations from the quark model, in particular the need for 2-body effects.

  1. Test of factorization in diffractive deep inelastic scattering and photoproduction at HERA

    SciTech Connect

    Polifka, Richard

    2015-04-10

    The QCD factorization theorem in diffraction is tested by comparing diffractive jet production data to QCD predictions based on fits to inclusive diffractive cross section data. H1 measured dijet production with a leading proton detected in the Very Forward Proton Spectrometer (VFPS), both in deep-inelastic scattering and in photoproduction. The DIS measurements are complemented by measurements of dijet production with an associated rapidity gap and in a data sample selected with a leading proton in the Forward Proton Spectrometer (FPS)

  2. Exclusive Photoproduction of K+Σ*-(n) Off Deuteron

    SciTech Connect

    Lu, Haiyun; Graham, Lewis; Zhao, Zhiwen; Park, Kijun; Gothe, Ralf

    2010-08-16

    We are using the CEBAF Large Acceptance Spectrometer (CLAS) to study the exclusive photoproduction of K+Σ*- (1385) off the deuteron. It will be the first published total cross section of this reaction channel. We show the preliminary results of the total cross section, while we present all the key steps of achieving it. In order to study the reaction mechanism, we also study and show the angular distribution in the Gottfried-Jackson frame.

  3. Pion photoproduction on /sup 27/Al: The effect of V/sub opt/

    SciTech Connect

    Freed, N.; Ostrander, P.

    1981-07-01

    Total cross sections for pion photoproduction to bound states in /sup 27/Mg are calculated in the distorted wave impulse approximation and compared to experiment. Particular attention is paid to the form of the optical potential used to incorporate final state effects. Substantial differences are found between results using an earlier potential and present results using potentials having different analytic forms. The latter lead to good agreement with experiment.

  4. Photoproduction of the eta prime meson in the effective Lagrangian approach

    SciTech Connect

    Mukhopadhyay, N.C.; Zhang, J.F.; Benmerrouche, M.

    1994-04-01

    In the framework of the effective Lagrangian approach, the authors study the {eta}{prime} photoproduction off protons, of great interest at CEBAF I and II. They calculate the contributions from the leading nucleon Born terms, vector meson exchanges, and estimate the resonance contributions, using the transition amplitudes from the recent quark model estimates by Capstick and Roberts. They discuss implications for the CEBAF experiments.

  5. Polarization Observables From The Photoproduction Of Omega-Mesons Using Linearly Polarized Photons

    SciTech Connect

    Martinez, Danny; Cole, Philip L.

    2014-01-01

    We report on the extraction of Polarization Observables Spin Density Matrix Elements (SDMEs), and Beam Asymmetry Sigma for omega meson photoproduction using a beam of linearly polarized photons in the photon energy region of Egamma = 1.3 to 1.7 GeV, by means of the angular distributions of the daughter pions from omega decay. These preliminary results are from the g8b dataset, which were collected with the CLAS detector in Hall B of Jefferson Lab.

  6. Preliminary results of t and f asymmetries for {eta} photoproduction from the proton

    SciTech Connect

    Tucker, Ross J.; Ritchie, Barry G.; Dugger, Michael R.

    2014-01-01

    We present preliminary data for the T and F asymmetries in {eta} photoproduction from the proton, along with comparisons to theoretical predictions. The data used in the present analysis were taken using the CEBAF Large Acceptance Spectrometer (CLAS) at Jefferson Lab, a cryogenic target which utilized transversely polarized protons in a butanol target, and incident tagged photons with energy between 0.62 and 2.93 GeV.

  7. Measurement of the G double-polarisation observable in pion photoproduction

    SciTech Connect

    J. McAndrew, D. Watts, E. Pasyuk

    2012-04-01

    The g9a experiment using the CLAS detector in Hall B of Jefferson Lab will measure double-polarization observables using a polarized energy-tagged photon beam in conjunction with the frozen spin target, FROST. This contribution describes the extraction of the G double polarization observable in the single pion photoproduction using a linearly polarized photon beam in the energy range 730-2300 MeV and the longitudinally polarized frozen spin target, FROST.

  8. A comparison of hydrogen photoproduction by sulfur-deprived Chlamydomonas reinhardtii under different growth conditions.

    PubMed

    Kosourov, Sergey; Patrusheva, Elena; Ghirardi, Maria L; Seibert, Michael; Tsygankov, Anatoly

    2007-03-10

    Continuous photoproduction of H(2) by the green alga, Chlamydomonas reinhardtii, is observed after incubating the cultures for about a day in the absence of sulfate and in the presence of acetate. Sulfur deprivation causes the partial and reversible inactivation of photosynthetic O(2) evolution in algae, resulting in the light-induced establishment of anaerobic conditions in sealed photobioreactors, expression of two [FeFe]-hydrogenases in the cells, and H(2) photoproduction for several days. We have previously demonstrated that sulfur-deprived algal cultures can produce H(2) gas in the absence of acetate, when appropriate experimental protocols were used (Tsygankov, A.A., Kosourov, S.N., Tolstygina, I.V., Ghirardi, M.L., Seibert, M., 2006. Hydrogen production by sulfur-deprived Chlamydomonas reinhardtii under photoautotrophic conditions. Int. J. Hydrogen Energy 31, 1574-1584). We now report the use of an automated photobioreactor system to compare the effects of photoautotrophic, photoheterotrophic and photomixotrophic growth conditions on the kinetic parameters associated with the adaptation of the algal cells to sulfur deprivation and H(2) photoproduction. This was done under the experimental conditions outlined in the above reference, including controlled pH. From this comparison we show that both acetate and CO(2) are required for the most rapid inactivation of photosystem II and the highest yield of H(2) gas production. Although, the presence of acetate in the system is not critical for the process, H(2) photoproduction under photoautotrophic conditions can be increased by optimizing the conditions for high starch accumulation. These results suggest ways of engineering algae to improve H(2) production, which in turn may have a positive impact on the economics of applied systems for H(2) production. PMID:17275940

  9. Energetics of Photoinduced Charge Migration within the Tryptophan Tetrad of an Animal (6-4) Photolyase.

    PubMed

    Cailliez, Fabien; Müller, Pavel; Firmino, Thiago; Pernot, Pascal; de la Lande, Aurélien

    2016-02-17

    Cryptochromes and photolyases are flavoproteins that undergo cascades of electron/hole transfers after excitation of the flavin cofactor. It was recently discovered that animal (6-4) photolyases, as well as animal cryptochromes, feature a chain of four tryptophan residues, while other members of the family contain merely a tryptophan triad. Transient absorption spectroscopy measurements on Xenopus laevis (6-4) photolyase have shown that the fourth residue is effectively involved in photoreduction but at the same time could not unequivocally ascertain the final redox state of this residue. In this article, polarizable molecular dynamics simulations and constrained density functional theory calculations are carried out to reveal the energetics of charge migration along the tryptophan tetrad. Migration toward the fourth tryptophan is found to be thermodynamically favorable. Electron transfer mechanisms are sought either through an incoherent hopping mechanism or through a multiple sites tunneling process. The Jortner-Bixon formulation of electron transfer (ET) theory is employed to characterize the hopping mechanism. The interplay between electron transfer and relaxation of protein and solvent is analyzed in detail. Our simulations confirm that ET in (6-4) photolyase proceeds out of equilibrium. Multiple site tunneling is modeled with the recently proposed flickering resonance mechanism. Given the position of energy levels and the distribution of electronic coupling values, tunneling over three tryptophan residues may become competitive in some cases, although a hopping mechanism is likely to be the dominant channel. For both reactive channels, computed rates are very sensitive to the starting protein configuration, suggesting that both can take place and eventually be mixed, depending on the state of the system when photoexcitation takes place.

  10. Proteomic analysis of hydrogen photoproduction in sulfur-deprived Chlamydomonas cells.

    PubMed

    Chen, Mei; Zhao, Le; Sun, Yong-Le; Cui, Su-Xia; Zhang, Li-Fang; Yang, Bin; Wang, Jie; Kuang, Ting-Yun; Huang, Fang

    2010-08-01

    The green alga Chlamydomonas reinhardtii is a model organism to study H(2) metabolism in photosynthetic eukaryotes. To understand the molecular mechanism of H(2) metabolism, we used 2-DE coupled with MALDI-TOF and MALDI-TOF/TOF-MS to investigate proteomic changes of Chlamydomonas cells that undergo sulfur-depleted H(2) photoproduction process. In this report, we obtained 2-D PAGE soluble protein profiles of Chlamydomonas at three time points representing different phases leading to H(2) production. We found over 105 Coomassie-stained protein spots, corresponding to 82 unique gene products, changed in abundance throughout the process. Major changes included photosynthetic machinery, protein biosynthetic apparatus, molecular chaperones, and 20S proteasomal components. A number of proteins related to sulfate, nitrogen and acetate assimilation, and antioxidative reactions were also changed significantly. Other proteins showing alteration during the sulfur-depleted H(2) photoproduction process were proteins involved in cell wall and flagella metabolisms. In addition, among these differentially expressed proteins, 11 were found to be predicted proteins without functional annotation in the Chlamydomonas genome database. The results of this proteomic analysis provide new insight into molecular basis of H(2) photoproduction in Chlamydomonas under sulfur depletion.

  11. The structure of an authentic spore photoproduct lesion in DNA suggests a basis for recognition.

    PubMed

    Singh, Isha; Jian, Yajun; Lian, Yajun; Li, Lei; Georgiadis, Millie M

    2014-03-01

    The spore photoproduct lesion (SP; 5-thymine-5,6-dihydrothymine) is the dominant photoproduct found in UV-irradiated spores of some bacteria such as Bacillus subtilis. Upon spore germination, this lesion is repaired in a light-independent manner by a specific repair enzyme: the spore photoproduct lyase (SP lyase). In this work, a host-guest approach in which the N-terminal fragment of Moloney murine leukemia virus reverse transcriptase (MMLV RT) serves as the host and DNA as the guest was used to determine the crystal structures of complexes including 16 bp oligonucleotides with and without the SP lesion at 2.14 and 1.72 Å resolution, respectively. In contrast to other types of thymine-thymine lesions, the SP lesion retains normal Watson-Crick hydrogen bonding to the adenine bases of the complementary strand, with shorter hydrogen bonds than found in the structure of the undamaged DNA. However, the lesion induces structural changes in the local conformation of what is otherwise B-form DNA. The region surrounding the lesion differs significantly in helical form from B-DNA, and the minor groove is widened by almost 3 Å compared with that of the undamaged DNA. Thus, these unusual structural features associated with SP lesions may provide a basis for recognition by the SP lyase. PMID:24598744

  12. Immobilized chloroplast-ferredoxin-hydrogenase system for the simultaneous photoproduction of hydrogen and oxygen

    SciTech Connect

    Woodward, J.; Greenbaum, E.

    1983-01-01

    The chloroplast-ferredoxin-hydrogenase (CFH) system is capable of the simultaneous photoproduction of hydrogen and oxygen using water as the source of electrons. The immobilization of chloroplasts and hydrogenase, which has been the subject of several reports has been attempted in order to increase their storage and functional stability. However, immobilized chloroplasts and hydrogenase have never been shown to produce hydrogen and oxygen simultaneously. The simultaneous photoproduction of hydrogen and oxygen is a valuable guide in assessing the role of water as the primary substrate in biophotolysis. As has been shown previously, the stoichiometric ratio of hydrogen to oxygen is, in general, not equal to 2. By measuring the oxygen that is produced along with the hydrogen, it is possible to set an upper limit on the photoproduced hydrogen that is derive d from water. The study reported here describes for the first time the simultaneous photoproduction of hydrogen and oxygen by the CFH system immobilized in calcium alginate gel spheres. 15 references, 4 figures.

  13. CFL3D Version 6.4-General Usage and Aeroelastic Analysis

    NASA Technical Reports Server (NTRS)

    Bartels, Robert E.; Rumsey, Christopher L.; Biedron, Robert T.

    2006-01-01

    This document contains the course notes on the computational fluid dynamics code CFL3D version 6.4. It is intended to provide from basic to advanced users the information necessary to successfully use the code for a broad range of cases. Much of the course covers capability that has been a part of previous versions of the code, with material compiled from a CFL3D v5.0 manual and from the CFL3D v6 web site prior to the current release. This part of the material is presented to users of the code not familiar with computational fluid dynamics. There is new capability in CFL3D version 6.4 presented here that has not previously been published. There are also outdated features no longer used or recommended in recent releases of the code. The information offered here supersedes earlier manuals and updates outdated usage. Where current usage supersedes older versions, notation of that is made. These course notes also provides hints for usage, code installation and examples not found elsewhere.

  14. Biological activity of photoproducts of merocyanine 540 generated by laser-light activation

    NASA Astrophysics Data System (ADS)

    Gulliya, Kirpal S.; Chanh, Tran C.; Pervaiz, Shazib; Harriman, Anthony; Matthews, James Lester

    1992-08-01

    Controlled exposure of photoactive compounds to light prior to their use in biological targets results in the formation of heretofore unknown photoproducts. This process of photoproduct generation, termed "preactivation," renders the photactive compound capable of systemic use without further dependence on light. Preactivation of mercyanin 540 (MC540) and several other photoactive compounds is achievable by exposure to CW and pulse laser radiation. The singlet oxygen generated at excited states attacks the dye molucule itself, resulting in the formation of biologically active photoproducts. For preactivated MC540 (photoproducts of MC540) generated by exposure to argon laser light (514 nm) and light from free-electron laser, we have demonstrated its effectiveness in selective killing of certain types of cultured tumor cells as well as human immunodeficiency virus type 1 (HIV-1) with very low, if any, damage to normal cells and tisues. For example, approximately 90% of the Burkitt's lymphoma Daudi cells and HL-60 leukemic cells are killed by preactivated MC540 at a concentration of 120 μg/ml. A two-hour treatment of cultured cells with buthionine sulfoxamine followed by the treatement with preactivated MC540 reults in 99.99% inhibition of clonogenic tumor stem cell growth. We also have demonstrated that preactivated MC540 is very effective in killing cell-free and cell-associated HIV-1. It also is very effective in killing HIV-1 and simian immunodeficiency virus (SIV) in virus-infected blood in vitro as determined by reverse transcriptase, P24, P17, core antigen expression and synctium formation. Treatment of HIV-1 with preactivated MC540 renders the treated HIV-1 incapable of binding to CD4 target molecules on T cells as determined by immunofluorescence and radioimmunoprecipitation assays. In vivo toxicology studies show that preactivated MC540 is very well tolerated and does not produce any signs of adverse reaction at the therapeutic doses, as determined by

  15. Results of a new OCTOPUS'' ECR ion source at 6. 4 GHz

    SciTech Connect

    Dupont, C.; Jongen, Y. ); Arakawa, K.; Yokota, W. ); Satoh, T.; Tachikawa, T. )

    1990-01-01

    The first OCTOPUS electron cyclstron resonance (ECR) multicharged heavy ion source was built in 1985 at the Centre de Recherches du Cyclotron of the University of Louvain (Belgium). This first source used an ECR frequency of 14.3 GHz in the injector stage and 8.5 GHz in the main confinement stage. A new OCTOPUS source has now been built for a new cyclotron to be installed at the Japan Atomic Energy Research Institute (JAERI). The design of this new OCTOPUS source is identical to the first OCTOPUS source, but uses an ECR frequency of 6.4 GHz in the main confinement stage. The experimental results are described, and a comparison is made between the two sources. However, the available data does not allow any clear conclusion to be drawn on frequency scaling.

  16. Hydrogen photoproduction is attenuated by disruption of an isoamylase gene in Chlamydomonas reinhardtii.

    PubMed

    Posewitz, Matthew C; Smolinski, Sharon L; Kanakagiri, Saradadevi; Melis, Anastasios; Seibert, Michael; Ghirardi, Maria L

    2004-08-01

    DNA insertional transformants of Chlamydomonas reinhardtii were screened chemochromically for attenuated H(2) production. One mutant, displaying low H(2) gas photoproduction, has a nonfunctional copy of a gene that shows high homology to the family of isoamylase genes found in several photosynthetic organisms. DNA gel blotting and gene complementation were used to link this isoamylase gene to previously characterized nontagged sta7 mutants. This mutant is therefore denoted sta7-10. In C. reinhardtii, the STA7 isoamylase gene is important for the accumulation of crystalline starch, and the sta7-10 mutant reported here contains <3% of the glucose found in insoluble starch when compared with wild-type control cells. Hydrogen photoproduction rates, induced after several hours of dark, anaerobic treatment, are attenuated in sta7 mutants. RNA gel blot analysis indicates that the mRNA transcripts for both the HydA1 and HydA2 [Fe]-hydrogenase genes are expressed in the sta7-10 mutant at greater than wild-type levels 0.5 h after anaerobic induction. However, after 1.5 h, transcript levels of both HydA1 and HydA2 begin to decline rapidly and reach nearly undetectable levels after 7 h. In wild-type cells, the hydrogenase transcripts accumulate more slowly, reach a plateau after 4 h of anaerobic treatment, and maintain the same level of expression for >7 h under anaerobic incubation. Complementation of mutant cells with genomic DNA corresponding to the STA7 gene restores both the starch accumulation and H(2) production phenotypes. The results indicate that STA7 and starch metabolism play an important role in C. reinhardtii H(2) photoproduction. Moreover, the results indicate that mere anaerobiosis is not sufficient to maintain hydrogenase gene expression without the underlying physiology, an important aspect of which is starch metabolism. PMID:15269330

  17. Nucleotide excision repair by dual incisions in plants

    PubMed Central

    Canturk, Fazile; Karaman, Muhammet; Selby, Christopher P.; Kemp, Michael G.; Kulaksiz-Erkmen, Gulnihal; Hu, Jinchuan; Li, Wentao; Lindsey-Boltz, Laura A.; Sancar, Aziz

    2016-01-01

    Plants use light for photosynthesis and for various signaling purposes. The UV wavelengths in sunlight also introduce DNA damage in the form of cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6-4) pyrimidone photoproducts [(6-4)PPs] that must be repaired for the survival of the plant. Genome sequencing has revealed the presence of genes for both CPD and (6-4)PP photolyases, as well as genes for nucleotide excision repair in plants, such as Arabidopsis and rice. Plant photolyases have been purified, characterized, and have been shown to play an important role in plant survival. In contrast, even though nucleotide excision repair gene homologs have been found in plants, the mechanism of nucleotide excision repair has not been investigated. Here we used the in vivo excision repair assay developed in our laboratory to demonstrate that Arabidopsis removes CPDs and (6-4)PPs by a dual-incision mechanism that is essentially identical to the mechanism of dual incisions in humans and other eukaryotes, in which oligonucleotides with a mean length of 26–27 nucleotides are removed by incising ∼20 phosphodiester bonds 5′ and 5 phosphodiester bonds 3′ to the photoproduct. PMID:27071131

  18. Nucleotide excision repair by dual incisions in plants.

    PubMed

    Canturk, Fazile; Karaman, Muhammet; Selby, Christopher P; Kemp, Michael G; Kulaksiz-Erkmen, Gulnihal; Hu, Jinchuan; Li, Wentao; Lindsey-Boltz, Laura A; Sancar, Aziz

    2016-04-26

    Plants use light for photosynthesis and for various signaling purposes. The UV wavelengths in sunlight also introduce DNA damage in the form of cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6-4) pyrimidone photoproducts [(6-4)PPs] that must be repaired for the survival of the plant. Genome sequencing has revealed the presence of genes for both CPD and (6-4)PP photolyases, as well as genes for nucleotide excision repair in plants, such as Arabidopsis and rice. Plant photolyases have been purified, characterized, and have been shown to play an important role in plant survival. In contrast, even though nucleotide excision repair gene homologs have been found in plants, the mechanism of nucleotide excision repair has not been investigated. Here we used the in vivo excision repair assay developed in our laboratory to demonstrate that Arabidopsis removes CPDs and (6-4)PPs by a dual-incision mechanism that is essentially identical to the mechanism of dual incisions in humans and other eukaryotes, in which oligonucleotides with a mean length of 26-27 nucleotides are removed by incising ∼20 phosphodiester bonds 5' and 5 phosphodiester bonds 3' to the photoproduct. PMID:27071131

  19. Physicochemical Mechanism of Light-Driven DNA Repair by (6-4) Photolyases

    NASA Astrophysics Data System (ADS)

    Faraji, Shirin; Dreuw, Andreas

    2014-04-01

    DNA photolyases are light-activated enzymes that repair DNA damage induced by ultraviolet (UV) radiation. UV radiation causes two of the most abundant mutagenic and cytotoxic DNA lesions: cyclobutane pyrimidine dimers and 6-4 photolesions. Photolyases selectively bind to DNA and initiate the splitting of mutagenic pyrimidine dimers via photoinduced electron transfer from a flavin adenine dinucleotide anion (FADH-) to the lesion triggering its repair. This review discusses the consecutive steps of the repair process, from both experimental and theoretical points of view. It covers the following issues: the process of how photolyases accommodate the lesion into their binding pockets, excitation energy transfer between two involved catalytic cofactors, photoinduced electron transfer to the lesion, the splitting of the pyrimidine dimer radical anion, and the fate of the unstable radical species created after the splitting of the thymine dimer. In particular, mechanisms of the splitting and restoration of the original bases are described in detail, and the most probable repair pathways are outlined.

  20. High-power MIXSEL: an integrated ultrafast semiconductor laser with 6.4 W average power.

    PubMed

    Rudin, B; Wittwer, V J; Maas, D J H C; Hoffmann, M; Sieber, O D; Barbarin, Y; Golling, M; Südmeyer, T; Keller, U

    2010-12-20

    High-power ultrafast lasers are important for numerous industrial and scientific applications. Current multi-watt systems, however, are based on relatively complex laser concepts, for example using additional intracavity elements for pulse formation. Moving towards a higher level of integration would reduce complexity, packaging, and manufacturing cost, which are important requirements for mass production. Semiconductor lasers are well established for such applications, and optically-pumped vertical external cavity surface emitting lasers (VECSELs) are most promising for higher power applications, generating the highest power in fundamental transverse mode (>20 W) to date. Ultrashort pulses have been demonstrated using passive modelocking with a semiconductor saturable absorber mirror (SESAM), achieving for example 2.1-W average power, sub-100-fs pulse duration, and 50-GHz pulse repetition rate. Previously the integration of both the gain and absorber elements into a single wafer was demonstrated with the MIXSEL (modelocked integrated external-cavity surface emitting laser) but with limited average output power (<200 mW). We have demonstrated the power scaling concept of the MIXSEL using optimized quantum dot saturable absorbers in an antiresonant structure design combined with an improved thermal management by wafer removal and mounting of the 8-µm thick MIXSEL structure directly onto a CVD-diamond heat spreader. The simple straight cavity with only two components has generated 28-ps pulses at 2.5-GHz repetition rate and an average output power of 6.4 W, which is higher than for any other modelocked semiconductor laser. PMID:21197032

  1. Asperity break after 12 years: The Mw6.4 2015 Lefkada (Greece) earthquake

    NASA Astrophysics Data System (ADS)

    Sokos, E.; Zahradník, J.; Gallovič, F.; Serpetsidaki, A.; Plicka, V.; Kiratzi, A.

    2016-06-01

    The Mw6.4 earthquake sequence of 2015 in western Greece is analyzed using seismic data. Multiple point source modeling, nonlinear slip patch, and linear slip inversions reveal a coherent rupture image with directivity toward the southwest and several moment release episodes, reflected in the complex aftershock distribution. The key feature is that the 2015 earthquake ruptured a strong asperity, which was left unbroken in between two large subevents of the Mw6.2 Lefkada doublet in 2003. This finding and the well-analyzed Cephalonia earthquake sequence of 2014 provide strong evidence of segmentation of the major dextral Cephalonia-Lefkada Transform Fault (CTF), being related to extensional duplex transform zones. We propose that the duplexes extend farther to the north and that the CTF runs parallel to the western coast of Lefkada and Cephalonia Islands, considerably closer to the inhabited islands than previously thought. Generally, this study demonstrates faulting complexity across short time scales (earthquake doublets) and long time scales (seismic gaps).

  2. Fatigue Behavior of Ti-6-4 Alloy with Application of Calcium Phosphate

    NASA Astrophysics Data System (ADS)

    Kamboj, Jaspal Singh

    It is key to consider a material's mechanical properties when determining its use for any given application, including biomaterial implantations. One such characteristic includes the fatigue life, which is determined by repeatedly cycling specified amounts of load on any given material. The fatigue life is then measured according to how many cycles of load the material can undergo before fracture. When a material is introduced to the human body it is usually expected that the life of the material will not match that of the patient. It is crucial to understand the fatigue life of the material before implantation to pre-diagnose how often and when the implant will need to be replaced. The purpose of this project is to help determine and compare the fatigue behaviors of a commonly used biocompatible coating on Titanium 6-4 metal alloy. The comparison will help identify how the material properties vary with the addition of calcium phosphate when compared to the bare alloy itself. Multiple, small rectangular samples were cut by electrical discharge machining (EDM) of which half were covered with calcium phosphate coating. Fatigue crack initiations and propagation would then be analyzed using scanning electron microscopy (SEM) to help determine the nature of the damage processes. It was found that the fatigue life of the coated samples varied at different stresses but was greater than the bare samples, and particularly high in the 900 MPa - 1000 MPa stress range.

  3. The Effect of Recent Pion Photoproduction Data on the SAID and MAID Analyses

    NASA Astrophysics Data System (ADS)

    Briscoe, William; Arndt, Richard; Paris, Mark; Strakovsky, Igor; Workman, Ronald

    2009-10-01

    Major experimental contributions to the SAID Pion Photoproduction data base from JLab, Mainz, Bonn, LNS, and Spring-8 have recently been publish or are about to be published. These data significantly constrain the SAID and MAID Partial-Wave Analyses. Yet there are still disagreements between the two analyses, especially in their predictions for double scattering channels. Three of these laboratories (JLab, Mainz, and Bonn) are in a position to perform ``complete'' experiments on complementary pion channels and in overlapping energy ranges. Latest Partial-Wave Analysis results will be compared to the latest published data and similarities and differences between SAID and MAID will be discussed.

  4. Helicity-dependent angular distributions in double-charged-pion photoproduction

    SciTech Connect

    Steffen Strauch

    2003-05-01

    Two-pion photoproduction in the reaction {gamma}p {yields} p{pi}{sup +} {pi}{sup -} has been studied at Jefferson Lab Hall B using a circularly-polarized tagged photon beam in the energy range between 0.6 GeV and 2.3 GeV. Owing to the large angular acceptance of the CLAS detector, complete beam-helicity-dependent angular distributions of the final-state particles were measured. The large cross-section asymmetries exhibit strong sensitivity to the kinematics of the reaction and provide valuable information on the reaction dynamics. Preliminary results are presented.

  5. A new measurement of Beam Asymmetry in Pion Photoproduction from the Neutron using CLAS

    SciTech Connect

    D. Sokhan, D. Watts, D. Branford, F. Klein

    2010-08-01

    We present a preliminary analysis of the photon beam asymmetry observable (Sigma) from the photoproduction reaction channel gamma+ n -> p + pi-. This new data was obtained using the near-4pi CEBAF Large Acceptance Spectrometer (CLAS) at Jefferson Laboratory, USA, employing a linearly polarised photon beam with an energy range 1.1 - 2.3 GeV. The measurement will provide new data to address the poorly established neutron excitation spectrum and will greatly expand the sparse world data-set both in energy and angle.

  6. Study of the Hyperon-Nucleon (YN) Interaction in Exclusive Λ Photoproduction off the Deuteron

    NASA Astrophysics Data System (ADS)

    Cao, Tongtong; Ilieva, Yordanka; Zachariou, Nicholas

    2016-07-01

    This study aims to extract the polarization observables Cx, Cz, ∑, Ox, and Oz for final-state interactions (FSI) in overrightarrow γ d to {K^ + }overrightarrow Λ n. The data were taken with the CEBAF Large Acceptance Spectrometer (CLAS) at the Thomas Jefferson National Accelerator Facility (JLab) during the E06-103 experiment. These are the very first results for FSI observables in hyperon photoproduction and are expected to constrain the free parameters of YN potentials. This work is funded in part by the U.S. NSF under grant PHY-125782.

  7. Inclusive Sigma- photoproduction on the neutron via the reaction gamma n (p) ---> K+ Sigma- (p)

    SciTech Connect

    Jorn Langheinrich; Ana Lima; Barry Berman

    2006-06-01

    The analysis described here is part of a comprehensive survey of the elementary strangeness photoproduction cross sections on the nucleon. The six elementary strangeness reactions are {gamma}n {yields} K{sup 0}{Lambda} and {gamma}p {yields} K{sup +}{Lambda} {gamma}n {yields} K{sup 0}{Sigma}{sup 0} and {gamma}p {yields} K{sup +}{Sigma}{sup 0} {gamma}n {yields} K{sup +}{Sigma}{sup -} and {gamma}p {yields} K{sup 0}{Sigma}|{sup +}

  8. Precise photoproduction of the charged top-pions at the LHC with forward detector acceptances

    NASA Astrophysics Data System (ADS)

    Sun, Hao; Yue, Chong-Xing

    2014-04-01

    We study the photoproduction of the charged top-pion predicted by the top triangle moose (TTM) model (a deconstructed version of the topcolor-assisted technicolor TC2 model) via the processes at the 14 TeV Large Hadron Collider (LHC) including next-to-leading order (NLO) QCD corrections. Our results show that the production cross sections and distributions are sensitive to the free parameters and . A typical QCD correction value is and this does not depend much on as well as the forward detector acceptances.

  9. Photoproduction of the Charged Top-Pions at the LHeC

    NASA Astrophysics Data System (ADS)

    Guo, Jing; Yue, Chong-Xing; Zhang, Jiao; Zeng, Qing-Guo

    2012-11-01

    The top triangle moose (TTM) model, which can be seen as the deconstructed version of the topcolor-assisted technicolor (TC2) model, predicts the existence of the charged top-pions πt± in low energy spectrum. In the context of this model, we consider photoproduction of πt± via the subprocesses γb → tπt- and at the large hadron-electron collider (LHeC), in which high energy photon beams are generated by using the Compton backscatting method. We find that, as long as the charged top-pions are not too heavy, they can be abundantly produced via 76 collision.

  10. Double neutral pion photoproduction off the proton with FOREST at ELPH

    NASA Astrophysics Data System (ADS)

    He, Q.; Fujimura, H.; Fukasawa, H.; Hashimoto, R.; Honda, Y.; Ishikawa, T.; Iwata, T.; Kaida, S.; Kasagi, J.; Kawano, A.; Kuwasaki, S.; Maeda, K.; Masumoto, S.; Miyabe, M.; Miyahara, F.; Mochizuki, K.; Muramatsu, N.; Nakamura, A.; Nawa, K.; Ogushi, S.; Okada, Y.; Onodera, Y.; Ozawa, K.; Sakamoto, Y.; Sato, M.; Shimizu, H.; Sugai, H.; Suzuki, K.; Tajima, Y.; Takahashi, S.; Taniguchi, Y.; Tsuchikawa, Y.; Yamazaki, H.; Yamazaki, R.; Yoshida, H. Y.

    2016-02-01

    Total cross section for the double neutral pion photoproduction off the proton is presented in the incident photon energy range of 0.58 to 1.15 GeV. The data were accumulated in the years 2009-2010, recorded by a 4π EM calorimeter at ELPH, named FOREST. The number of recorded events obtained during this period is 1.6×109 for a hydrogen target. Two neutral pions are reconstructed via detecting their decay products, four gammas (2π0 → 4γ). Compared to the previous results obtained by other groups, our data are in good agreement with theirs within error bars.

  11. Effects of the Consistent Interaction on Kaon Photoproduction with Spin 5/2 Nucleon Resonances

    NASA Astrophysics Data System (ADS)

    Clymton, S.; Mart, T.

    2016-08-01

    Theoretical models for kaon photoproduction with spin 5/2 nucleon resonances have been plagued with the problem of interaction consistency. A number of studies predicted that a model with a consistent interaction leads to a better agreement with data. In this study a model with consistent interaction (model 2) is compared to the old model, which utilizes an inconsistent interaction (model 1), as well as to experimental data. The unknown parameters in scattering amplitude are extracted from fitting to 7400 experimental data points. This is performed by minimizing the X2/N value. It is found that model with a consistent interaction (model 2) is more suitable for explaining experimental data.

  12. The GlueX experiment: Search for gluonic excitations via photoproduction at Jefferson Lab

    SciTech Connect

    Eugenio, Paul

    2013-07-01

    Studies of meson spectra via strong decays provide insight regarding QCD at the confinement scale. These studies have led to phenomenological models for QCD such as the constituent quark model. However, QCD allows for a much richer spectrum of meson states which include extra states such as exotics, hybrids, multi-quarks, and glueballs. First discussion of the status of exotic meson searches is given followed by an overview of the progress at Jefferson Lab to double the energy of the machine to 12 GeV, which will allow us to access photoproduction of mesons in search for gluonic excited states.

  13. Studying the Pc(4450) resonance in J/ψ photoproduction off protons

    DOE PAGESBeta

    Blin, A. N. Hiller; Fernandez-Ramirez, C.; Jackura, A.; Mathieu, V.; Mokeev, V. I.; Pilloni, A.; Szczepaniak, A. P.

    2016-08-01

    In this study, a resonance-like structure, the Pc(4450), has recently been observed in the J/ψ p spectrum by the LHCb collaboration. We discuss the feasibility of detecting this structure in J/ψ photoproduction in the CLAS12 experiment at JLab. We present a first estimate of the upper limit for the branching ratio of the Pc(4450) to J/ψ p. Our estimates, which take into account the experimental resolution effects, lead to a sizable cross section close to the J/ψ production threshold, which makes future experiments covering this region very promising.

  14. Evidence for nucleon-resonance excitation in omega-meson photoproduction.

    PubMed

    Ajaka, J; Assafiri, Y; Bouchigny, S; Didelez, J P; Fichen, L; Guidal, M; Hourany, E; Kouznetsov, V; Kunne, R; Mushkarenkov, A N; Nedorezov, V; Rudnev, N; Turinge, A; Zhao, Q

    2006-04-01

    The photoproduction of the omega meson has been studied at GRAAL from threshold up to a photon energy of 1.5 GeV. The differential cross sections and beam asymmetries have been measured precisely at all angles. The total cross section is also obtained. Systematic enhancements of the differential cross section at large angles and nonzero beam asymmetries at intermediate angles provide clear evidence for s- and u-channel resonant processes. The data are compared to the results of hadron and quark models.

  15. Mm/submm Study of Gas-Phase Photoproducts from Methanol Interstellar Ice Analogues

    NASA Astrophysics Data System (ADS)

    Mesko, AJ; Smith, Houston Hartwell; Milam, Stefanie N.; Widicus Weaver, Susanna L.

    2016-06-01

    Icy grain reactions have gained quite the popularity in the astrochemistry community to explain the formation of complex organic molecules. Through temperature programmed desorption and photolysis experiments we use rotational spectroscopy to measure the gas-phase products of icy grain reactions. Previous results include testing detection limits of the system by temperature programmed desorption of methanol and water ices, photochemistry of gas-phase methanol, and detection of photodesorbed water from a pure water ice surface. Current work that will be discussed focuses on the detection of gas-phase CO and other photoproducts from an ice surface.

  16. Σ(1385) photoproduction from the proton within a Regge-plus-resonance approach

    NASA Astrophysics Data System (ADS)

    He, Jun

    2014-05-01

    The interaction mechanism of the Σ(1385) photoproduction from proton γp →K+Σ0(1385) is investigated within a Regge-plus-resonance approach based on the experimental data released by the CLAS Collaboration recently. The t channel and the u channel are responsible for the behaviors of differential cross sections at forward and backward angles, respectively. The contributions from nucleon resonances including N* and Δ*, which are determined by the predicted decay amplitudes in the constituent quark model, are found to be small, but the F35 state Δ (2000) is essential to reproduce the differential cross section.

  17. Regge approach to charged-pion photoproduction at invariant energies above 2 GeV

    SciTech Connect

    Sibirtsev, A; Haidenbauer, J; Krewald, S; Lee, T S.H.; Meissner, U -G; Thomas, A W

    2007-10-01

    A Regge model with absorptive corrections is employed in a global analysis of the world data on positive and negative pion photoproduction for photon energies from 3 to 8~GeV. In this region resonance contributions are expected to be negligible so that the available experimental information on differential cross sections and single polarization observables at $-t{\\leq}2$ GeV$^2$ allows us to determine the non-resonant part of the reaction amplitude reliably. The model amplitude is then used to predict observables for photon energies below $3$ GeV. Differences between our predictions and data in this energy region are systematically examined as possible signals for the presence of excited baryons. We find that the data available for the polarized photon asymmetry show promising resonance signatures at invariant energies around 2~GeV. With regard to differential cross sections the analysis of negative pion photoproduction data, obtained recently at JLab, indicates likewise the presence of resonance structures around 2~GeV.

  18. First measurement of direct $f_0(980)$ photoproduction on the proton

    SciTech Connect

    Battaglieri, Marco; De Vita, Raffaella; Szczepaniak, Adam

    2009-03-01

    We report on the results of the first measurement of exclusive $f_0(980)$ meson photoproduction on protons for $E_\\gamma=3.0 - 3.8$ GeV and $-t = 0.4-1.0$ GeV$^2$. Data were collected with the CLAS detector at the Thomas Jefferson National Accelerator Facility. The resonance was detected via its decay in the $\\pi^+ \\pi^-$ channel by performing a partial wave analysis of the reaction $\\gamma p \\to p \\pi^+ \\pi^-$. Clear evidence of the $f_0(980)$ meson was found in the interference between $P$ and $S$ waves at $M_{\\pi^+ \\pi^-}\\sim 1$ GeV. The $S$-wave differential cross section integrated in the mass range of the $f_0(980)$ was found to be a factor of 50 smaller than the cross section for the $\\rho$ meson. This is the first time the $f_0(980)$ meson has been measured in a photoproduction experiment.

  19. Photoproduction of the f1(1285)/eta(1295) Mesons using CLAS at Jefferson Lab

    NASA Astrophysics Data System (ADS)

    Dickson, Ryan

    This work presents the results of analysis of a meson of mass mx = 1281.0 +/- 0.8 MeV/c² and a FWHM of Gammax = 18.4 +/- 1.4 MeV/ c² seen in CLAS at Jefferson Lab in photoproduction off the proton, gammap → xp. The f1(1285), eta(1295) or both are candidates for this observed state. Neither of these states has previously been observed in photoproduction. The meson was measured in the decay modes x → etapi +pi-, K+ K¯0pi-, K -K0pi+, and rho 0gamma through detection of its charged decay products and the recoil proton. Differential cross sections were obtained via the x → etapi +pi-, K+ K¯0pi-, and K -K0pi + decay channels from threshold up to a center-of-mass energy of 2.8 GeV, and compared to recent Regge model predictions. Dalitz analysis showed strong evidence for the f1(1285) identity, with the dominance of etapi+pi- decays via a+/-0 (980)pi∓ intermediate state and with interference consistent with a spin one particle. The relative branching fractions Gamma( KK¯pi)/Gamma(etapipi) and Gamma(rho0gamma)/Gamma(etapipi) were measured, with agreement to world data for the f 1(1285) in the former and lower than the world average for the latter.

  20. Photochemistry and Photobiology of the Spore Photoproduct: A 50-Year Journey.

    PubMed

    Setlow, Peter; Li, Lei

    2015-11-01

    Fifty years ago, a new thymine dimer was discovered as the dominant DNA photolesion in UV-irradiated bacterial spores [Donnellan, J. E. & Setlow R. B. (1965) Science, 149, 308-310], which was later named the spore photoproduct (SP). Formation of SP is due to the unique environment in the spore core that features low hydration levels favoring an A-DNA conformation, high levels of calcium dipicolinate that acts as a photosensitizer, and DNA saturation with small, acid-soluble proteins that alters DNA structure and reduces side reactions. In vitro studies reveal that any of these factors alone can promote SP formation; however, SP formation is usually accompanied by the production of other DNA photolesions. Therefore, the nearly exclusive SP formation in spores is due to the combined effects of these three factors. Spore photoproduct photoreaction is proved to occur via a unique H-atom transfer mechanism between the two involved thymine residues. Successful incorporation of SP into an oligonucleotide has been achieved via organic synthesis, which enables structural studies that reveal minor conformational changes in the SP-containing DNA. Here, we review the progress on SP photochemistry and photobiology in the past 50 years, which indicates a very rich SP photobiology that may exist beyond endospores.

  1. Resummation of Large Endpoint Corrections to Color-Octet J/psi Photoproduction

    SciTech Connect

    Sean Fleming; Adam K. Leibovich; Thomas Mehen

    2006-07-27

    An unresolved problem in J/{psi} phenomenology is a systematic understanding of the differential photoproduction cross section, d{sigma}/dz [{gamma} + p {yields} J/{psi} + X], where z = E{sub {psi}}/E{sub {gamma}} in the proton rest frame. In the non-relativistic QCD (NRQCD) factorization formalism, fixed-order perturbative calculations of color-octet mechanisms suffer from large perturbative and nonperturbative corrections that grow rapidly in the endpoint region, z {yields} 1. In this paper, NRQCD and soft collinear effective theory are combined to resum these large corrections to the color-octet photoproduction cross section. We derive a factorization theorem for the endpoint differential cross section involving the parton distribution function and the color-octet J/{psi} shape functions. A one loop matching calculation explicitly confirms our factorization theorem at next-to-leading order. Large perturbative corrections are resummed using the renormalization group. The calculation of the color-octet contribution to d{sigma}/dz is in qualitative agreement with data. Quantitative tests of the universality of color-octet matrix elements require improved knowledge of shape functions entering these calculations as well as resummation of the color-singlet contribution which accounts for much of the total cross section and also peaks near the endpoint.

  2. Measurement of direct f0(980) photoproduction on the proton.

    PubMed

    Battaglieri, M; De Vita, R; Szczepaniak, A P; Adhikari, K P; Aghasyan, M; Amaryan, M J; Ambrozewicz, P; Anghinolfi, M; Asryan, G; Avakian, H; Bagdasaryan, H; Baillie, N; Ball, J P; Baltzell, N A; Batourine, V; Bedlinskiy, I; Bellis, M; Benmouna, N; Berman, B L; Bibrzycki, L; Biselli, A S; Bookwalter, C; Bouchigny, S; Boiarinov, S; Bradford, R; Branford, D; Briscoe, W J; Brooks, W K; Bültmann, S; Burkert, V D; Calarco, J R; Careccia, S L; Carman, D S; Casey, L; Chen, S; Cheng, L; Clinton, E; Cole, P L; Collins, P; Crabb, D; Crannell, H; Crede, V; Cummings, J P; Dale, D; Daniel, A; Dashyan, N; De Masi, R; De Sanctis, E; Degtyarenko, P V; Deur, A; Dhamija, S; Dharmawardane, K V; Dickson, R; Djalali, C; Dodge, G E; Donnelly, J; Doughty, D; Dugger, M; Dzyubak, O P; Egiyan, H; Egiyan, K S; El Fassi, L; Elouadrhiri, L; Eugenio, P; Fedotov, G; Fersch, R; Forest, T A; Fradi, A; Gabrielyan, M Y; Gan, L; Garçon, M; Gasparian, A; Gavalian, G; Gevorgyan, N; Gilfoyle, G P; Giovanetti, K L; Girod, F X; Glamazdin, O; Goett, J; Goetz, J T; Gohn, W; Golovatch, E; Gordon, C I O; Gothe, R W; Graham, L; Griffioen, K A; Guidal, M; Guler, N; Guo, L; Gyurjyan, V; Hadjidakis, C; Hafidi, K; Hakobyan, H; Hakobyan, R S; Hanretty, C; Hardie, J; Hassall, N; Heddle, D; Hersman, F W; Hicks, K; Hleiqawi, I; Holtrop, M; Hyde, C E; Ilieva, Y; Ireland, D G; Ishkhanov, B S; Isupov, E L; Ito, M M; Jenkins, D; Jo, H S; Johnstone, J R; Joo, K; Juengst, H G; Kageya, T; Kalantarians, N; Keller, D; Kellie, J D; Khandaker, M; Khetarpal, P; Kim, W; Klein, A; Klein, F J; Klimenko, A V; Konczykowski, P; Kossov, M; Krahn, Z; Kramer, L H; Kubarovsky, V; Kuhn, J; Kuhn, S E; Kuleshov, S V; Kuznetsov, V; Lachniet, J; Laget, J M; Langheinrich, J; Lawrence, D; Lee, T; Lesniak, L; Li, Ji; Livingston, K; Lowry, M; Lu, H Y; Maccormick, M; Malace, S; Markov, N; Mattione, P; McCracken, M E; McKinnon, B; Mecking, B A; Melone, J J; Mestayer, M D; Meyer, C A; Mibe, T; Mikhailov, K; Mineeva, T; Minehart, R; Mirazita, M; Miskimen, R; Mochalov, V; Mokeev, V; Moreno, B; Moriya, K; Morrow, S A; Moteabbed, M; Munevar, E; Mutchler, G S; Nadel-Turonski, P; Nakagawa, I; Nasseripour, R; Niccolai, S; Niculescu, G; Niculescu, I; Niczyporuk, B B; Niroula, M R; Niyazov, R A; Nozar, M; Osipenko, M; Ostrovidov, A I; Park, K; Park, S; Pasyuk, E; Paris, M; Paterson, C; Pereira, S Anefalos; Pierce, J; Pivnyuk, N; Pocanic, D; Pogorelko, O; Pozdniakov, S; Price, J W; Prok, Y; Protopopescu, D; Raue, B A; Riccardi, G; Ricco, G; Ripani, M; Ritchie, B G; Rosner, G; Rossi, P; Sabatié, F; Saini, M S; Salamanca, J; Salgado, C; Sandorfi, A; Santoro, J P; Sapunenko, V; Schott, D; Schumacher, R A; Serov, V S; Sharabian, Y G; Sharov, D; Shvedunov, N V; Smith, E S; Smith, L C; Sober, D I; Sokhan, D; Starostin, A; Stavinsky, A; Stepanyan, S; Stepanyan, S S; Stokes, B E; Stoler, P; Stopani, K A; Strakovsky, I I; Strauch, S; Taiuti, M; Tedeschi, D J; Teymurazyan, A; Tkabladze, A; Tkachenko, S; Todor, L; Tur, C; Ungaro, M; Vineyard, M F; Vlassov, A V; Watts, D P; Wei, X; Weinstein, L B; Weygand, D P; Williams, M; Wolin, E; Wood, M H; Yegneswaran, A; Yurov, M; Zana, L; Zhang, J; Zhao, B; Zhao, Z W

    2009-03-13

    We report on the results of the first measurement of exclusive f_{0}(980) meson photoproduction on protons for E_{gamma}=3.0-3.8 GeV and -t=0.4-1.0 GeV2. Data were collected with the CLAS detector at the Thomas Jefferson National Accelerator Facility. The resonance was detected via its decay in the pi;{+}pi;{-} channel by performing a partial wave analysis of the reaction gammap-->ppi;{+}pi;{-}. Clear evidence of the f_{0}(980) meson was found in the interference between P and S waves at M_{pi;{+}pi;{-}} approximately 1 GeV. The S-wave differential cross section integrated in the mass range of the f_{0}(980) was found to be a factor of about 50 smaller than the cross section for the rho meson. This is the first time the f_{0}(980) meson has been measured in a photoproduction experiment. PMID:19392104

  3. Resummation of large endpoint corrections to color-octet J/{psi} photoproduction

    SciTech Connect

    Fleming, Sean; Leibovich, Adam K.; Mehen, Thomas

    2006-12-01

    An unresolved problem in J/{psi} phenomenology is a systematic understanding of the differential photoproduction cross section, d{sigma}/dz[{gamma}+p{yields}J/{psi}+X], where z=E{sub {psi}}/E{sub {gamma}} in the proton rest frame. In the nonrelativistic QCD (NRQCD) factorization formalism, fixed-order perturbative calculations of color-octet mechanisms suffer from large perturbative and nonperturbative corrections that grow rapidly in the endpoint region, z{yields}1. In this paper, NRQCD and soft collinear effective theory are combined to resum these large corrections to the color-octet photoproduction cross section. We derive a factorization theorem for the endpoint differential cross section involving the parton distribution function and the color-octet J/{psi} shape functions. A one-loop matching calculation explicitly confirms our factorization theorem at next-to-leading order. Large perturbative corrections are resummed using the renormalization group. The calculation of the color-octet contribution to d{sigma}/dz is in qualitative agreement with data. Quantitative tests of the universality of color-octet matrix elements require improved knowledge of shape functions entering these calculations as well as resummation of the color-singlet contribution which accounts for much of the total cross section and also peaks near the endpoint.

  4. Exclusive J/ψ and ϒ photoproduction and the low x gluon

    NASA Astrophysics Data System (ADS)

    Jones, S. P.; Martin, A. D.; Ryskin, M. G.; Teubner, T.

    2016-04-01

    We study exclusive vector meson photoproduction, γ p\\to V+p with V=J/\\psi or ϒ, at next-to-leading order (NLO) in collinear factorisation, in order to examine what may be learnt about the gluon distribution at very low x. We examine the factorisation scale dependence of the predictions. We argue that, using knowledge of the NLO corrections, terms enhanced by a large {ln}(1/ξ ) can be reabsorbed in the LO part by a choice of the factorisation scale. (In these exclusive processes ξ takes the role of Bjorken-x.) Then, the scale dependence coming from the remaining NLO contributions has no {ln}(1/ξ ) enhancements. As a result, we find that predictions for the amplitude of ϒ production are stable to within about ±15%. This will allow data for the exclusive process {pp}\\to p{{\\Upsilon }}p at the Large Hadron Collider (LHC), particularly from LHCb, to be included in global parton analyses to constrain the gluon parton distribution function (PDF) down to x∼ {10}-5. Moreover, the study of exclusive J/\\psi photoproduction indicates that the gluon density found in the recent global PDF analyses is too small at low x and low scales.

  5. Solution Spectroscopic and Chemical Properties of the Complex Hydride [FeH(6)](4-).

    PubMed

    Linn, Donald E.; Gibbins, Sidney G.

    1997-07-30

    Solution spectroscopic and chemical behavior was examined in the case of the homoleptic hydridic anion of iron [FeH(6)](4)(-). Examination of the UV-visible spectrum in THF revealed a LMCT band which occurs at 41 x 10(3) cm(-)(1) (epsilon = 1200 L mol(-)(1) cm(-)(1)). A manifold between 470 and 500 nm was consistent with overlapping spin-forbidden transitions: (1)A(1g) --> (3)T(2g) and (1)A(1g) --> (3)T(1g). The doubly spin-forbidden transition ((1)A(1g) --> (5)T(2g)) was not observed. Spin-allowed ligand field transitions, (1)A(1g) --> (1)T(2g) and (1)A(1g) --> (1)T(1g), occurred at 28.2 (epsilon = 356 L mol(-)(1) cm(-)(1)) and 24.2 x 10(3) cm(-)(1) (epsilon = 414 L mol(-)(1) cm(-)(1)), respectively. The latter data yielded the parameters Delta(H)()- = 25 x 10(3) cm(-)(1) and B = 310 cm(-)(1), assuming C/B = 4. Thus, the position of hydride was established in the spectrochemical series of low-spin Fe(2+) as well beneath cyanide (35 x 10(3) cm(-)(1) ) yet well above bipyridine (18 x 10(3) cm(-)(1) ). Titration of solutions of [FeH(6])[MgX(THF)(2)](4) (1.2 x 10(-)(3) M), I (X = Cl, Br), with [MgCl(2)] ((1.8-45) x 10(-)(3) M) did not perturb the ligand field absorptions but caused a hypsochromic shift in the LMCT band consistent with the formation of the less anionic polyhydride complex, I, from [MgX(THF)(n)()](+) and {[FeH(6)][MgX(THF)(2)](3)}(-), II, where K(1)()() approximately (3 +/- 1) x 10(-)(3) (UV-visible). The (1)H NMR (1.2 x 10(-)(3) M, 25 degrees C) in THF-d(8) displayed two hydride components at delta -20.3 and -20.4 ppm (5.6:1). Coalescence of the two hydride absorptions occurred near 40 degrees C and 200 MHz. Reaction of I with (6)LiOH (8 equiv) was found by (6)Li{(1)H} NMR to result in the replacement of the [MgX](+) unit in I with (6)Li(+).

  6. Hydrogen peroxide and superoxide photoproduction in diverse marine waters: A simple proxy for estimating direct CO2 photochemical fluxes

    NASA Astrophysics Data System (ADS)

    Powers, Leanne C.; Miller, William L.

    2015-09-01

    The photoproduction of H2O2 and CO2 exhibits a well-defined relationship with an average CO2:H2O2 molar ratio of 6.6 ± 1.8 as determined in a variety of marine waters ranging from a dark tidal creek to clear offshore stations near the Gulf Stream. Even when corrected for photobleaching, accumulation of both H2O2 and CO2 was not linear beyond 12 h of constant irradiation, with interval rates indicating that production efficiency for both products decreased with increasing photon dose. Direct measurements of O2- photoproduction, together with its dark thermal decay to H2O2, indicate that O2- may be the better proxy for photochemical dissolved organic matter oxidation to CO2. Given the very short irradiation times needed to determine O2- production rates (~2-5 min), determination of O2- photoproduction may provide robust estimates for initial CO2 photoproduction rates even in blue water, allowing greatly improved estimates for the global significance of dissolved organic carbon photooxidation.

  7. Significant Increase in Hydrogen Photoproduction Rates and Yields by Wild-Type Algae is Detected at High Photobioreactor Gas Phase Volume (Fact Sheet)

    SciTech Connect

    Not Available

    2012-07-01

    This NREL Hydrogen and Fuel Cell Technical Highlight describes how hydrogen photoproduction activity in algal cultures can be improved dramatically by increasing the gas-phase to liquid-phase volume ratio of the photobioreactor. NREL, in partnership with subcontractors from the Institute of Basic Biological Problems in Pushchino, Russia, demonstrated that the hydrogen photoproduction rate in algal cultures always decreases exponentially with increasing hydrogen partial pressure above the culture. The inhibitory effect of high hydrogen concentrations in the photobioreactor gas phase on hydrogen photoproduction by algae is significant and comparable to the effect observed with some anaerobic bacteria.

  8. Maximizing the Hydrogen Photoproduction Yields in Chlamydomonas Reinhardtii Cultures: The Effect of the H2 Partial Pressure

    SciTech Connect

    Kosourov, S. N.; Batyrova, K. A.; Petushkova, E. P.; Tsygankov, A. A.; Ghirardi, M. L.; Seibert, M.

    2012-05-01

    Photoproduction of H{sub 2} gas has been examined in sulfur/phosphorus-deprived Chalmydomonas reinhardtii cultures, placed in photobioreactors (PhBRs) with different gas phase to liquid phase ratios (V{sub g.p.}/V{sub l.p.}). The results demonstrate that an increase in the ratio stimulates H{sub 2} photoproduction activity in both algal suspension cultures and in algae entrapped in thin alginate films. In suspension cultures, a 4x increase (from {approx}0.5 to {approx}2) in V{sub g.p.}/V{sub l.p} results in a 2x increase (from 10.8 to 23.1 mmol l{sup -1} or 264-565 ml l{sup -1}) in the total yield of H{sub 2} gas. Remarkably, 565 ml of H{sub 2} gas per liter of the suspension culture is the highest yield ever reported for a wild-type strain in a time period of less than 190 h. In immobilized algae, where diffusion of H{sub 2} from the medium to the PhBR gas phase is not affected by mixing, the maximum rate and yield of H{sub 2} photoproduction occur in PhBRs with V{sub g.p.}/V{sub l.p} above 7 or in a PhBR with smaller headspace, if the H{sub 2} is effectively removed from the medium by continuous flushing of the headspace with argon. These experiments in combination with studies of the direct inhibitory effect of high H{sub 2} concentrations in the PhBR headspace on H{sub 2} photoproduction activity in algal cultures clearly show that H{sub 2} photoproduction in algae depends significantly on the partial pressure of H{sub 2} (not O{sub 2} as previously thought) in the PhBR gas phase.

  9. A TeGM6-4r antigen-based immunochromatographic test (ICT) for animal trypanosomosis.

    PubMed

    Nguyen, Thu-Thuy; Ruttayaporn, Ngasaman; Goto, Yasuyuki; Kawazu, Shin-ichiro; Sakurai, Tatsuya; Inoue, Noboru

    2015-11-01

    Animal trypanosomosis is a disease that is distributed worldwide which results in huge economic losses due to reduced animal productivity. Endemic regions are often located in the countryside where laboratory diagnosis is costly or inaccessible. The establishment of simple, effective, and accurate field tests is therefore of great interest to the farming and veterinary sectors. Our study aimed to develop a simple, rapid, and sensitive immunochromatographic test (ICT) for animal trypanosomosis utilizing the recombinant tandem repeat antigen TeGM6-4r, which is conserved amongst salivarian trypanosome species. In the specificity analysis, TeGM6-4r/ICT detected all of Trypanosoma evansi-positive controls from experimentally infected water buffaloes. As expected, uninfected controls tested negative. All sera samples collected from Tanzanian and Ugandan cattle that were Trypanosoma congolense- and/or Trypanosoma vivax-positive by microscopic examination of the buffy coat were found to be positive by the newly developed TeGM6-4r/ICT, which was comparable to results from TeGM6-4r/ELISA (kappa coefficient [κ] = 0.78). TeGM6/ICT also showed substantial agreement with ELISA using Trypanosoma brucei brucei (κ = 0.64) and T. congolense (κ = 0.72) crude antigen, suggesting the high potential of TeGM6-4r/ICT as a field diagnostic test, both for research purposes and on-site diagnosis of animal trypanosomosis.

  10. Genome-wide analysis of human global and transcription-coupled excision repair of UV damage at single-nucleotide resolution.

    PubMed

    Hu, Jinchuan; Adar, Sheera; Selby, Christopher P; Lieb, Jason D; Sancar, Aziz

    2015-05-01

    We developed a method for genome-wide mapping of DNA excision repair named XR-seq (excision repair sequencing). Human nucleotide excision repair generates two incisions surrounding the site of damage, creating an ∼30-mer. In XR-seq, this fragment is isolated and subjected to high-throughput sequencing. We used XR-seq to produce stranded, nucleotide-resolution maps of repair of two UV-induced DNA damages in human cells: cyclobutane pyrimidine dimers (CPDs) and (6-4) pyrimidine-pyrimidone photoproducts [(6-4)PPs]. In wild-type cells, CPD repair was highly associated with transcription, specifically with the template strand. Experiments in cells defective in either transcription-coupled excision repair or general excision repair isolated the contribution of each pathway to the overall repair pattern and showed that transcription-coupled repair of both photoproducts occurs exclusively on the template strand. XR-seq maps capture transcription-coupled repair at sites of divergent gene promoters and bidirectional enhancer RNA (eRNA) production at enhancers. XR-seq data also uncovered the repair characteristics and novel sequence preferences of CPDs and (6-4)PPs. XR-seq and the resulting repair maps will facilitate studies of the effects of genomic location, chromatin context, transcription, and replication on DNA repair in human cells.

  11. Site-specific analysis of UV-induced cyclobutane pyrimidine dimers in nucleotide excision repair-proficient and -deficient hamster cells: Lack of correlation with mutational spectra.

    PubMed

    Vreeswijk, Maaike P G; Meijers, Caro M; Giphart-Gassler, Micheline; Vrieling, Harry; van Zeeland, Albert A; Mullenders, Leon H F; Loenen, Wil A M

    2009-04-26

    Irradiation of cells with UVC light induces two types of mutagenic DNA photoproducts, i.e. cyclobutane pyrimidine dimers (CPD) and pyrimidine (6-4) pyrimidone photoproducts (6-4 PP). To investigate the relationship between the frequency of UV-induced photolesions at specific sites and their ability to induce mutations, we quantified CPD formation at the nucleotide level along exons 3 and 8 of the hprt gene using ligation-mediated PCR, and determined the mutational spectrum of 132 UV-induced hprt mutants in the AA8 hamster cell line and of 165 mutants in its nucleotide excision repair-defective derivative UV5. In AA8 cells, transversions predominated with a strong strand bias towards thymine-containing photolesions in the non-transcribed strand. As hamster AA8 cells are proficient in global genome repair of 6-4 PP but selectively repair CPD from the transcribed strand of active genes, most mutations probably resulted from erroneous bypass of CPD in the non-transcribed strand. However, the relative incidence of CPD and the positions where mutations most frequently arose do not correlate. In fact some major damage sites hardly gave rise to the formation of mutations. In the repair-defective UV5 cells, mutations were almost exclusively C>T transitions caused by photoproducts at PyC sites in the transcribed strand. Even though CPD were formed at high frequencies at some TT sites in UV5, these photoproducts did not contribute to mutation induction at all. We conclude that, even in the absence of repair, large variations in the level of induction of CPD at different sites throughout the two exons do not correspond to frequencies of mutation induction.

  12. Chiral effective-field theory in the {delta}(1232) region. II. Radiative pion photoproduction

    SciTech Connect

    Pascalutsa, Vladimir; Vanderhaeghen, Marc

    2008-01-01

    We present a theoretical study of the radiative pion photoproduction on the nucleon ({gamma}N{yields}{pi}N{gamma}{sup '}) in the {delta}-resonance region, with the aim to determine the magnetic dipole moment (MDM) of the {delta}{sup +}(1232). The study is done within the framework of chiral effective-field theory, where the expansion is performed (to next-to-leading order) in the {delta} power-counting scheme, an extension of chiral perturbation theory to the {delta}-resonance energy region. We present the results for the absorptive part of the {delta} MDM, as well as perform a sensitivity study of the dependence of {gamma}N{yields}{pi}N{gamma}{sup '} observables on the real part of the {delta} MDM. We find that an asymmetry for circular polarization of the photon beam may provide a model-independent way to measure the {delta} MDM.

  13. Further studies of the photoproduction of isolated photons with a jet at HERA

    NASA Astrophysics Data System (ADS)

    Abramowicz, H.; Abt, I.; Adamczyk, L.; Adamus, M.; Aggarwal, R.; Antonelli, S.; Arslan, O.; Aushev, V.; Aushev, Y.; Bachynska, O.; Barakbaev, A. N.; Bartosik, N.; Behnke, O.; Behr, J.; Behrens, U.; Bertolin, A.; Bhadra, S.; Bloch, I.; Bokhonov, V.; Boos, E. G.; Borras, K.; Brock, I.; Brugnera, R.; Bruni, A.; Brzozowska, B.; Bussey, P. J.; Caldwell, A.; Capua, M.; Catterall, C. D.; Chwastowski, J.; Ciborowski, J.; Ciesielski, R.; Cooper-Sarkar, A. M.; Corradi, M.; Corriveau, F.; D'Agostini, G.; Dementiev, R. K.; Devenish, R. C. E.; Dolinska, G.; Drugakov, V.; Dusini, S.; Ferrando, J.; Figiel, J.; Foster, B.; Gach, G.; Garfagnini, A.; Geiser, A.; Gizhko, A.; Gladilin, L. K.; Gogota, O.; Golubkov, Yu. A.; Grebenyuk, J.; Gregor, I.; Grzelak, G.; Gueta, O.; Guzik, M.; Hain, W.; Hartner, G.; Hochman, D.; Hori, R.; Ibrahim, Z. A.; Iga, Y.; Ishitsuka, M.; Iudin, A.; Januschek, F.; Kadenko, I.; Kananov, S.; Kanno, T.; Karshon, U.; Kaur, M.; Kaur, P.; Khein, L. A.; Kisielewska, D.; Klanner, R.; Klein, U.; Kondrashova, N.; Kononenko, O.; Korol, Ie.; Korzhavina, I. A.; Kotanski, A.; Kötz, U.; Kovalchuk, N.; Kowalski, H.; Kuprash, O.; Kuze, M.; Levchenko, B. B.; Levy, A.; Libov, V.; Limentani, S.; Lisovyi, M.; Lobodzinska, E.; Lohmann, W.; Löhr, B.; Lohrmann, E.; Longhin, A.; Lontkovskyi, D.; Lukina, O. Yu.; Maeda, J.; Makarenko, I.; Malka, J.; Martin, J. F.; Mergelmeyer, S.; Idris, F. Mohamad; Mujkic, K.; Myronenko, V.; Nagano, K.; Nigro, A.; Nobe, T.; Notz, D.; Nowak, R. J.; Olkiewicz, K.; Onishchuk, Yu.; Paul, E.; Perlanski, W.; Perrey, H.; Pokrovskiy, N. S.; Proskuryakov, A. S.; Przybycien, M.; Raval, A.; Roloff, P.; Rubinsky, I.; Ruspa, M.; Samojlov, V.; Saxon, D. H.; Schioppa, M.; Schmidke, W. B.; Schneekloth, U.; Schörner-Sadenius, T.; Schwartz, J.; Shcheglova, L. M.; Shevchenko, R.; Shkola, O.; Singh, I.; Skillicorn, I. O.; Slominski, W.; Sola, V.; Solano, A.; Spiridonov, A.; Stanco, L.; Stefaniuk, N.; Stern, A.; Stewart, T. P.; Stopa, P.; Sztuk-Dambietz, J.; Szuba, D.; Szuba, J.; Tassi, E.; Temiraliev, T.; Tokushuku, K.; Tomaszewska, J.; Trofymov, A.; Trusov, V.; Tsurugai, T.; Turcato, M.; Turkot, O.; Tymieniecka, T.; Verbytskyi, A.; Viazlo, O.; Walczak, R.; Abdullah, W. A. T. Wan; Wichmann, K.; Wing, M.; Wolf, G.; Yamada, S.; Yamazaki, Y.; Zakharchuk, N.; Żarnecki, A. F.; Zawiejski, L.; Zenaiev, O.; Zhautykov, B. O.; Zhmak, N.; Zotkin, D. S.

    2014-08-01

    In this extended analysis using the ZEUS detector at HERA, the photoproduction of isolated photons together with a jet is measured for different ranges of the fractional photon energy, x {/γ meas}, contributing to the photon-jet final state. Cross sections are evaluated in the photon transverse-energy and pseudorapidity ranges 6 < E {/T γ } < 15 GeV and -0 .7 < η γ < 0 .9, and for jet transverse-energy and pseudorapidity ranges 4 < E {/T jet} < 35 GeV and -1 .5 < η jet < 1 .8, for an integrated luminosity of 374 pb-1. The kinematic observables studied comprise the transverse energy and pseudorapidity of the photon and the jet, the azimuthal difference between them, the fraction of proton energy taking part in the interaction, and the difference between the pseudorapidities of the photon and the jet. Higher-order theoretical calculations are compared to the results.

  14. Measurement of tropospheric 300 nm solar ultraviolet flux for determination of O/1D/ photoproduction rate

    NASA Technical Reports Server (NTRS)

    Sellers, B.; Hanser, F. A.

    1978-01-01

    The tropospheric importance of the OH radical, and the reaction scheme that leads to its formation, are now being widely investigated. Ozone photolysis at wavelengths no greater than 318 nm produces O(1D), a small fraction of which then reacts with water vapor to yield OH. Although experimental data are available for the O(1D) quantum yield, as well as the O3 absorption cross section, all previous tropospheric photochemical models have had to use theoretical calculations to determine the UV flux. Discussed in this paper are aircraft spectral measurements of the solar UV flux at two altitudes - 2 and 6 km. These results have been compared with three theoretical approaches. The measured experimental fluxes have been combined here with recent quantum yield data to calculate the O(1D) photoproduction rate for various albedo values. This rate is larger than that used in models by about a factor of 2 for reasonable values of assumed albedo.

  15. Polarization Transfer in Wide-Angle Compton Scattering and Single-Pion Photoproduction from the Proton

    NASA Astrophysics Data System (ADS)

    Fanelli, C.; Cisbani, E.; Hamilton, D. J.; Salmé, G.; Wojtsekhowski, B.; Ahmidouch, A.; Annand, J. R. M.; Baghdasaryan, H.; Beaufait, J.; Bosted, P.; Brash, E. J.; Butuceanu, C.; Carter, P.; Christy, E.; Chudakov, E.; Danagoulian, S.; Day, D.; Degtyarenko, P.; Ent, R.; Fenker, H.; Fowler, M.; Frlez, E.; Gaskell, D.; Gilman, R.; Horn, T.; Huber, G. M.; de Jager, C. W.; Jensen, E.; Jones, M. K.; Kelleher, A.; Keppel, C.; Khandaker, M.; Kohl, M.; Kumbartzki, G.; Lassiter, S.; Li, Y.; Lindgren, R.; Lovelace, H.; Luo, W.; Mack, D.; Mamyan, V.; Margaziotis, D. J.; Markowitz, P.; Maxwell, J.; Mbianda, G.; Meekins, D.; Meziane, M.; Miller, J.; Mkrtchyan, A.; Mkrtchyan, H.; Mulholland, J.; Nelyubin, V.; Pentchev, L.; Perdrisat, C. F.; Piasetzky, E.; Prok, Y.; Puckett, A. J. R.; Punjabi, V.; Shabestari, M.; Shahinyan, A.; Slifer, K.; Smith, G.; Solvignon, P.; Subedi, R.; Wesselmann, F. R.; Wood, S.; Ye, Z.; Zheng, X.

    2015-10-01

    Wide-angle exclusive Compton scattering and single-pion photoproduction from the proton have been investigated via measurement of the polarization transfer from a circularly polarized photon beam to the recoil proton. The wide-angle Compton scattering polarization transfer was analyzed at an incident photon energy of 3.7 GeV at a proton scattering angle of θcmp=70 ° . The longitudinal transfer KLL, measured to be 0.645 ±0.059 ±0.048 , where the first error is statistical and the second is systematic, has the same sign as predicted for the reaction mechanism in which the photon interacts with a single quark carrying the spin of the proton. However, the observed value is ˜3 times larger than predicted by the generalized-parton-distribution-based calculations, which indicates a significant unknown contribution to the scattering amplitude.

  16. Extraction of electromagnetic properties of the {Delta}(1232) excitation from pion photoproduction

    SciTech Connect

    Wilbois, T.; Wilhelm, P.; Arenhoevel, H.

    1998-01-01

    Several methods for the treatment of pion photoproduction in the region of the {Delta}(1232) resonance are discussed, in particular the effective Lagrangian approach and the speed plot analysis are compared to a dynamical treatment. As a main topic, we discuss the extraction of the genuine resonance parts of the magnetic dipole and electric quadrupole multipoles of the electromagnetic excitation of the resonance. To this end, we try to relate the various values for the ratio R{sub EM} of the E2 to M1 multipole excitation strengths for the {Delta}(1232) resonance as extracted by the different methods to corresponding ratios of a dynamical model. Moreover, it is confirmed that all methods for extracting resonance properties suffer from an unitary ambiguity which is due to some phenomenological contributions entering the models. {copyright} {ital 1998} {ital The American Physical Society}

  17. Dalitz Decay of Pseudoscalar Mesons from Photoproduction on Hydrogen Target with CLAS

    NASA Astrophysics Data System (ADS)

    Kunkel, Michael; Amaryan, Moskov; Paolone, Michael; Weygand, Dennis; CLAS Collaboration

    2013-10-01

    Experimental results on the Dalitz decay of pseudoscalar mesons P(π0 , η ,η') -->e+e- γ produced in the photoproduction reaction γ + p --> P(π0 , η , η ') + p on a Hydrogen target, with CLAS setup at Thomas Jefferson National Accelerator Facility (TJNAF), are presented. The total statistics collected for π0 , η Dalitz decay exceeds the world's published statistics by an order of magnitude, while the Dalitz decay of η' -->e+e- γ is observed and measured for the first time. The data obtained will allow for measurements of transition form factors that are encoded in the mass spectrum of the e+e- system. The current status of analysis of the π0 , η transition form factors and the search for the heavy photon from the e+e- mass system will be discussed.

  18. Penrose photoproduction processes - A high efficiency energy mechanism for active galactic nuclei and quasars

    NASA Technical Reports Server (NTRS)

    Leiter, D.; Kafatos, M.

    1979-01-01

    Recent observations of NGC 4151 and 3C273 suggest that the nuclei of active galaxies have very high gamma ray efficiencies. In addition, optical studies of M87 have indicated the possibility of a massive black hole in its central region. The above facts have led to study of a new physical mechanism, Penrose Photoproduction Processes, in the ergospheres of massive Kerr black holes, as a way to account for the fluctuating, high efficiency, energy production associated with active galaxies and quasars. Observational signatures, associated with this mechanism, occur in the form of approximately 2 MeV and approximately 2 GeV gamma ray cutoffs which might be corroborated by the observed spectra of NGC 4151 and 3C273, respectively.

  19. BFKL evolution and the growth with energy of exclusive J /Ψ and ϒ photoproduction cross sections

    NASA Astrophysics Data System (ADS)

    Bautista, I.; Fernandez Tellez, A.; Hentschinski, M.

    2016-09-01

    We investigate whether the Balitsky-Fadin-Kuraev-Lipatov (BFKL) low x evolution equation is capable to describe the energy dependence of the exclusive photo-production cross-section of vector mesons J /Ψ and ϒ on protons. Such cross-sections have been measured by both HERA experiments H1 and ZEUS in electron-proton collisions and by LHC experiments ALICE, CMS and LHCb in ultra-peripheral proton-proton and ultra-peripheral proton-lead collisions. Our approach provides a perturbative description of the rise with energy and relies only on a fit of the initial transverse momentum profile of the proton impact factor, which can be extracted from BFKL fits to inclusive HERA data. We find that BFKL evolution is capable to provide a very good description of the energy dependence of the current data set, while the available fits of the proton impact factor require an adjustment in the overall normalization.

  20. Exclusive photoproduction of J/ψ and ψ(2S) in pp and AA collisions

    SciTech Connect

    Cisek, Anna; Schäfer, Wolfgang; Szczurek, Antoni

    2015-04-10

    The amplitude for γp → J/ψp(γp → ψ'p) is calculated in a pQCD k{sub ⊥}-factorization approach. The total cross section for this process is calculated for different unintegrated gluon distributions and compared with the HERA data and the data extracted recently by the LHCb collaboration. The amplitude for γp → J/ψp(γp → ψ'p) is used to predict the cross section for exclusive photoproduction of the J/ψ(ψ') meson in proton-proton and nucleus-nucleus collisions. In the pp case, compared to earlier calculations we include both Dirac and Pauli electromagnetic form factors. We also discuss the dependence of nuclear shadowing on the charmonium state.

  1. Photochemistry. Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure.

    PubMed

    Premi, Sanjay; Wallisch, Silvia; Mano, Camila M; Weiner, Adam B; Bacchiocchi, Antonella; Wakamatsu, Kazumasa; Bechara, Etelvino J H; Halaban, Ruth; Douki, Thierry; Brash, Douglas E

    2015-02-20

    Mutations in sunlight-induced melanoma arise from cyclobutane pyrimidine dimers (CPDs), DNA photoproducts that are typically created picoseconds after an ultraviolet (UV) photon is absorbed at thymine or cytosine. We found that in melanocytes, CPDs are generated for >3 hours after exposure to UVA, a major component of the radiation in sunlight and in tanning beds. These "dark CPDs" constitute the majority of CPDs and include the cytosine-containing CPDs that initiate UV-signature C→T mutations. Dark CPDs arise when UV-induced reactive oxygen and nitrogen species combine to excite an electron in fragments of the pigment melanin. This creates a quantum triplet state that has the energy of a UV photon but induces CPDs by energy transfer to DNA in a radiation-independent manner. Melanin may thus be carcinogenic as well as protective against cancer. These findings also validate the long-standing suggestion that chemically generated excited electronic states are relevant to mammalian biology.

  2. Beam-Helicity Asymmetries in Double-Charged-Pion Photoproduction on the Proton

    SciTech Connect

    S. Strauch; B. L. Berman

    2005-08-01

    Beam-helicity asymmetries for the two-pion-photoproduction reaction gamma + p --> p pi+ pi- have been studied for the first time in the resonance region for center-of-mass energies between 1.35 GeV and 2.30 GeV. The experiment was performed at Jefferson Lab with the CEBAF Large Acceptance Spectrometer using circularly polarized tagged photons incident on an unpolarized hydrogen target. Beam-helicity-dependent angular distributions of the final-state particles were measured. The large cross-section asymmetries exhibit strong sensitivity to the kinematics and dynamics of the reaction. The data are compared with the results of various phenomenological model calculations, and show that these models currently do not provide an adequate description for the behavior of this new observable.

  3. Polarization transfer in wide-angle Compton scattering and single-pion photoproduction from the proton

    DOE PAGESBeta

    Fanelli, Cristiano V.

    2015-10-06

    Wide-angle exclusive Compton scattering and single-pion photoproduction from the proton have been investigated via measurement of the polarization transfer from a circularly polarized photon beam to the recoil proton. The WACS polarization transfer was analyzed at an incident photon energy of 3.7 GeV at a proton scattering angle of θPcm = 70°. The longitudinal transfer KLL, measured to be 0.645 ± 0.059 ± 0.048, where the first error is statistical and the second is systematic, has the same sign as predicted for the reaction mechanism in which the photon interacts with a single quark carrying the spin of the proton.more » However, the observed value is ~3 times larger than predicted by the GPD-based calculations, which indicates a significant unknown contribution to the scattering amplitude.« less

  4. Polarization transfer in wide-angle Compton scattering and single-pion photoproduction from the proton

    SciTech Connect

    Fanelli, Cristiano V.

    2015-10-06

    Wide-angle exclusive Compton scattering and single-pion photoproduction from the proton have been investigated via measurement of the polarization transfer from a circularly polarized photon beam to the recoil proton. The WACS polarization transfer was analyzed at an incident photon energy of 3.7 GeV at a proton scattering angle of θPcm = 70°. The longitudinal transfer KLL, measured to be 0.645 ± 0.059 ± 0.048, where the first error is statistical and the second is systematic, has the same sign as predicted for the reaction mechanism in which the photon interacts with a single quark carrying the spin of the proton. However, the observed value is ~3 times larger than predicted by the GPD-based calculations, which indicates a significant unknown contribution to the scattering amplitude.

  5. Effect of the Spin 3/2 Nucleon Resonances in Kaon Photoproduction

    NASA Astrophysics Data System (ADS)

    Arifi, A. J.; Mart, T.

    2016-08-01

    We have studied two different formulations of spin 3/2 nucleon resonance by means of kaon photoproduction on the proton γp→K+Λ. The formulations of spin 3/2 nucleon resonances proposed by Adelseck (model A) and Pascalutsa (model B) have been used in deriving the scattering amplitudes. The amplitudes are calculated by means of the relevant Feynman diagrams for the process. All nucleon resonances with spin up to 3/2 listed by the Particle Data Group are included in the model. Both formulations are then compared with the experimental data, which include differential cross section and polarization observables, through X2 minimization. It is found that the Pascalutsa's formulation of the spin 3/2 leads to a better agreement with the experimental data.

  6. Differential Photoproduction Cross Sections of the Sigma0(1385), Lambda(1405), and Lambda(1520)

    SciTech Connect

    Moriya, Kei; Schumacher, Reinhard A.

    2013-10-01

    We report the exclusive photoproduction cross sections for the Sigma(1385), Lambda(1405), and Lambda(1520) in the reactions gamma + p -> K+ + Y* using the CLAS detector for energies from near the respective production thresholds up to a center-of-mass energy W of 2.85 GeV. The differential cross sections are integrated to give the total exclusive cross sections for each hyperon. Comparisons are made to current theoretical models based on the effective Lagrangian approach and fitted to previous data. The accuracy of these models is seen to vary widely. The cross sections for the Lambda(1405) region are strikingly different for the Sigma+pi-, Sigma0 pi0, and Sigma- pi+ decay channels, indicating the effect of isospin interference, especially at W values close to the threshold.

  7. Scaling behavior in exclusive meson photoproduction from Jefferson Lab at large momentum transfers

    SciTech Connect

    Dey, Biplab

    2014-07-01

    With the availability of new high-statistics and wide-angle measurements for several exclusive non-πN meson photoproduction channels from Jefferson Lab, we examine the fundamental scaling law of 90° scattering in QCD that was originally derived in the high-energy perturbative limit. The data show scaling to be prominently visible even in the medium-energy domain of 2.5 GeV ≲√s≲2.84 GeV, where √s is the center-of-mass energy. While constituent quark exchange suffices for pseudoscalar mesons, additional gluon exchanges from higher Fock states of the hadronic wave functions appear be needed for vector-meson production. Finally, the case of the Φ(1020), where two-gluon exchanges are known to dominate, is especially illuminating.

  8. Measurement of the transverse target and beam-target asymmetries in η meson photoproduction at MAMI.

    PubMed

    Akondi, C S; Annand, J R M; Arends, H J; Beck, R; Bernstein, A; Borisov, N; Braghieri, A; Briscoe, W J; Cherepnya, S; Collicott, C; Costanza, S; Downie, E J; Dieterle, M; Fix, A; Fil'kov, L V; Garni, S; Glazier, D I; Gradl, W; Gurevich, G; Hall Barrientos, P; Hamilton, D; Hornidge, D; Howdle, D; Huber, G M; Kashevarov, V L; Keshelashvili, I; Kondratiev, R; Korolija, M; Krusche, B; Lazarev, A; Lisin, V; Livingston, K; MacGregor, I J D; Mancel, J; Manley, D M; Martel, P; McNicoll, E F; Meyer, W; Middleton, D; Miskimen, R; Mushkarenkov, A; Nefkens, B M K; Neganov, A; Nikolaev, A; Oberle, M; Ostrick, M; Ortega, H; Ott, P; Otte, P B; Oussena, B; Pedroni, P; Polonski, A; Polyanski, V V; Prakhov, S; Reicherz, G; Rostomyan, T; Sarty, A; Schumann, S; Steffen, O; Strakovsky, I I; Strub, Th; Supek, I; Tiator, L; Thomas, A; Unverzagt, M; Usov, Yu A; Watts, D P; Werthmüller, D; Witthauer, L; Wolfes, M

    2014-09-01

    We present new data for the transverse target asymmetry T and the very first data for the beam-target asymmetry F in the γ[over →]p[over →]→ηp reaction up to a center-of-mass energy of W=1.9  GeV. The data were obtained with the Crystal-Ball/TAPS detector setup at the Glasgow tagged photon facility of the Mainz Microtron MAMI. All existing model predictions fail to reproduce the new data indicating a significant impact on our understanding of the underlying dynamics of η meson photoproduction. The peculiar nodal structure observed in existing T data close to threshold is not confirmed. PMID:25238349

  9. {phi}-Meson Photoproduction with Linearly Polarized Photons at Threshold Energies

    SciTech Connect

    Salamanca, Julian; Cole, Philip L.

    2007-10-26

    The observables provided by linearly-polarized photons are of interest in delineating the contributions of the various hadronic processes giving rise to vector meson photoproduction. In particular, we describe how {phi}-meson production affords an incisive tool for exploring the nature of the parity exchange at threshold energies, the strangeness content of proton, as well as extracting signatures for the violation of Okubo-Zweig-Iizuka observation (OZI rule). Our goal is to study the {gamma}-vectorp{yields}{phi}p reaction, with {phi}{yields}K{sup +}K{sup -}, in the photon energy range of 1.7 to 2.1 GeV by using the Coherent Linear Bremsstrahlung Facility in Hall B of Jefferson Laboratory, Newport News, VA. The data were collected during the g8b run in the summer of 2005.

  10. Cascade spectroscopy in photoproduction on a proton target at Jefferson Lab

    SciTech Connect

    L. Guo; D. P. Weygand

    2005-10-01

    The CLAS Collaboration at Jefferson Lab conducted a photoproduction experiment on a proton target using a tagged photon beam with an energy range of 1.5-3.8 GeV during May-July 2004. With an integrated luminosity of about 75 pb{sup -1}, this experiment provides the largest data set for photon-proton reactions ever collected. The reaction {gamma}p {yields} K{sup +}K{sup +}{Xi}{sup -}(1320) has been investigated and the preliminary results of the cross section measurement of {Xi}{sup -}(1320) for the photon energy range of 2.7-3.8 GeV have been obtained. In a search for excited cascade states, the reaction of {gamma}p {yields} K{sup +}K{sup +}{pi}{sup -}({Xi}{sup 0}(1320)) has also been explored.

  11. Extraction of polarization observables in vector-meson photoproduction using a polarized target at CLAS

    NASA Astrophysics Data System (ADS)

    Roy, P.

    2016-05-01

    Baryon spectroscopy is an indispensable tool for investigating the nature of strong interaction within baryons. The study of vector-meson photoproduction is expected to improve our understanding of the properties of known baryon resonances and also immensely aid in discovering new higher-mass resonances. Polarization observables, in addition to unpolarized cross sections, are required to identify the contributing resonances to these reactions with minimal ambiguities. Preliminary results from the FROST experiment on beam-polarization observables in γ →p →→p ω as well as γ →p →→p π+π- using a linearly-polarized beam and a transversely-polarized FROzen Spin butanol Target will be presented. The latter reaction will provide important information on N* to (broad) ρ vector-meson decay modes, which is difficult to extract directly from the data.

  12. First measurement of the helicity asymmetry E in η photoproduction on the proton

    NASA Astrophysics Data System (ADS)

    Senderovich, I.; Morrison, B. T.; Dugger, M.; Ritchie, B. G.; Pasyuk, E.; Tucker, R.; Brock, J.; Carlin, C.; Keith, C. D.; Meekins, D. G.; Seely, M. L.; Rönchen, D.; Döring, M.; Collins, P.; Adhikari, K. P.; Adikaram, D.; Akbar, Z.; Anderson, M. D.; Anefalos Pereira, S.; Badui, R. A.; Ball, J.; Baltzell, N. A.; Battaglieri, M.; Batourine, V.; Bedlinskiy, I.; Biselli, A. S.; Boiarinov, S.; Briscoe, W. J.; Brooks, W. K.; Burkert, V. D.; Carman, D. S.; Celentano, A.; Chandavar, S.; Charles, G.; Colaneri, L.; Cole, P. L.; Contalbrigo, M.; Cortes, O.; Credé, V.; D'Angelo, A.; Dashyan, N.; De Vita, R.; De Sanctis, E.; Deur, A.; Djalali, C.; Dupre, R.; Egiyan, H.; El Alaoui, A.; El Fassi, L.; Elouadrhiri, L.; Eugenio, P.; Fedotov, G.; Fegan, S.; Filippi, A.; Fleming, J. A.; Fradi, A.; Garillon, B.; Ghandilyan, Y.; Gilfoyle, G. P.; Giovanetti, K. L.; Girod, F. X.; Glazier, D. I.; Goetz, J. T.; Gohn, W.; Golovatch, E.; Gothe, R. W.; Griffioen, K. A.; Guidal, M.; Guo, L.; Hafidi, K.; Hakobyan, H.; Hanretty, C.; Hattawy, M.; Hicks, K.; Ho, D.; Holtrop, M.; Hughes, S. M.; Ilieva, Y.; Ireland, D. G.; Ishkhanov, B. S.; Jenkins, D.; Jiang, H.; Jo, H. S.; Joo, K.; Joosten, S.; Keller, D.; Khachatryan, G.; Khandaker, M.; Kim, A.; Klein, F. J.; Kubarovsky, V.; Kunkel, M. C.; Lenisa, P.; Livingston, K.; Lu, H. Y.; MacGregor, I. J. D.; Mattione, P.; McKinnon, B.; Meyer, C. A.; Mineeva, T.; Mokeev, V.; Montgomery, R. A.; Movsisyan, A.; Munoz Camacho, C.; Nadel-Turonski, P.; Net, L. A.; Niccolai, S.; Niculescu, G.; Niculescu, I.; Osipenko, M.; Park, K.; Park, S.; Peng, P.; Phelps, W.; Pisano, S.; Pogorelko, O.; Price, J. W.; Prok, Y.; Puckett, A. J. R.; Ripani, M.; Rizzo, A.; Rosner, G.; Roy, P.; Sabatié, F.; Salgado, C.; Schott, D.; Schumacher, R. A.; Seder, E.; Simonyan, A.; Skorodumina, Iu.; Smith, G. D.; Sober, D. I.; Sparveris, N.; Stepanyan, S.; Stoler, P.; Strakovsky, I. I.; Strauch, S.; Sytnik, V.; Tian, Ye; Ungaro, M.; Voskanyan, H.; Voutier, E.; Walford, N. K.; Wei, X.; Wood, M. H.; Zachariou, N.; Zana, L.; Zhang, J.; Zhao, Z. W.; Zonta, I.

    2016-04-01

    Results are presented for the first measurement of the double-polarization helicity asymmetry E for the η photoproduction reaction γp → ηp. Data were obtained using the FROzen Spin Target (FROST) with the CLAS spectrometer in Hall B at Jefferson Lab, covering a range of center-of-mass energy W from threshold to 2.15 GeV and a large range in center-of-mass polar angle. As an initial application of these data, the results have been incorporated into the Jülich-Bonn model to examine the case for the existence of a narrow N* resonance between 1.66 and 1.70 GeV. The addition of these data to the world database results in marked changes in the predictions for the E observable from that model. Further comparison with several theoretical approaches indicates these data will significantly enhance our understanding of nucleon resonances.

  13. Φ-Meson Photoproduction with Linearly Polarized Photons at Threshold Energies

    SciTech Connect

    Salamanca, Julian; Cole, Philip L

    2007-10-01

    The observables provided by linearly-polarized photons are of interest in delineating the contributions of the various hadronic processes giving rise to vector meson photoproduction. In particular, we describe how Φ-meson production affords an incisive tool for exploring the nature of the parity exchange at threshold energies, the strangeness content of proton, as well as extracting signatures for the violation of Okubo-Zweig-Iizuka observation (OZI rule). Our goal is to study the γp → Φp reaction, with Φ → K+K-, in the photon energy range of 1.7 to 2.1 GeV by using the Coherent Linear Bremsstrahlung Facility in Hall B of Jefferson Laboratory, Newport News, VA. The data were collected during the g8b run in the summer of 2005.

  14. First measurement of coherent $\\phi$-meson photoproduction on deuteron at low energies

    SciTech Connect

    Tsutomu Mibe; Haiyan Gao; Kenneth Hicks; Kevin Kramer; Stepan Stepanyan; David Tedeschi; Moscov Amaryan; Pawel Ambrozewicz; Marco Anghinolfi; G. Asryan; Gerard Audit; Harutyun Avakian; Hovhannes Baghdasaryan; Nathan Baillie; Jacques Ball; Nathan Baltzell; Marco Battaglieri; Ivan Bedlinski; Ivan Bedlinskiy; Matthew Bellis; Nawal Benmouna; Barry Berman; Angela Biselli; Lukasz Blaszczyk; Sylvain Bouchigny; Sergey Boyarinov; Robert Bradford; Derek Branford; William Briscoe; William Brooks; Stephen Bueltmann; Volker Burkert; Cornel Butuceanu; John Calarco; Sharon Careccia; Daniel Carman; Shifeng Chen; Philip Cole; Patrick Collins; Philip Coltharp; Donald Crabb; Hall Crannell; Volker Crede; John Cummings; Natalya Dashyan; Rita De Masi; Raffaella De Vita; Enzo De Sanctis; Pavel Degtiarenko; Alexandre Deur; Kahanawita Dharmawardane; Richard Dickson; Chaden Djalali; Gail Dodge; Joseph Donnelly; David Doughty; Michael Dugger; Oleksandr Dzyubak; Hovanes Egiyan; Kim Egiyan; Lamiaa Elfassi; Latifa Elouadrhiri; Paul Eugenio; Gleb Fedotov; Gerald Feldman; Herbert Funsten; Michel Garcon; Gagik Gavalian; Gerard Gilfoyle; Kevin Giovanetti; Francois-Xavier Girod; John Goetz; Atilla Gonenc; Christopher Gordon; Ralf Gothe; Keith Griffioen; Michel Guidal; Nevzat Guler; Lei Guo; Vardan Gyurjyan; Cynthia Hadjidakis; Kawtar Hafidi; Hayk Hakobyan; Rafael Hakobyan; Charles Hanretty; John Hardie; F. Hersman; Ishaq Hleiqawi; Maurik Holtrop; Charles Hyde; Charles Hyde-Wright; Yordanka Ilieva; David Ireland; Boris Ishkhanov; Eugeny Isupov; Mark Ito; David Jenkins; Hyon-Suk Jo; John Johnstone; Kyungseon Joo; Henry Juengst; Narbe Kalantarians; James Kellie; Mahbubul Khandaker; Wooyoung Kim; Andreas Klein; Franz Klein; Alexei Klimenko; Mikhail Kossov; Zebulun Krahn; Laird Kramer; V. Kubarovsky; Joachim Kuhn; Sebastian Kuhn; Sergey Kuleshov; Viacheslav Kuznetsov; Jeff Lachniet; Jean Laget; Jorn Langheinrich; David Lawrence; Tsung-shung Lee; Ji Li; Kenneth Livingston; Haiyun Lu; Marion MacCormick; Claude Marchand; Nikolai Markov; Paul Mattione; Bryan McKinnon; Bernhard Mecking; Joseph Melone; Mac Mestayer; Curtis Meyer; Konstantin Mikhaylov; Ralph Minehart; Marco Mirazita; Rory Miskimen; Viktor Mokeev; Kei Moriya; Steven Morrow; M. Moteabbed; E. Munevar; Gordon Mutchler; P. Nadel-Turonski; Rakhsha Nasseripour; Silvia Niccolai; Gabriel Niculescu; Maria-Ioana Niculescu; Bogdan Niczyporuk; Megh Niroula; Rustam Niyazov; Mina Nozar; Mikhail Osipenko; Alexander Ostrovidov; K. Park; Evgueni Pasyuk; Craig Paterson; S. Anefalos Pereira; Joshua Pierce; Nikolay Pivnyuk; Dinko Pocanic; Oleg Pogorelko; S. Pozdniakov; John Price; Yelena Prok; Dan Protopopescu; Brian Raue; Gregory Riccardi; Giovanni Ricco; Marco Ripani; Barry Ritchie; Federico Ronchetti; Guenther Rosner; Patrizia Rossi; Franck Sabatie; Julian Salamanca; Carlos Salgado; Joseph Santoro; Vladimir Sapunenko; Reinhard Schumacher; Vladimir Serov; Youri Sharabian; Dmitri Sharov; Nikolay Shvedunov; Elton Smith; Lee Smith; Daniel Sober; Daria Sokhan; A. Stavinsky; Samuel Stepanyan; B.E. Stokes; Paul Stoler; I.I. Strakovsky; Steffen Strauch; Mauro Taiuti; Ulrike Thoma; Avtandil Tkabladze; Svyatoslav Tkachenko; Luminita Todor; Clarisse Tur; Maurizio Ungaro; Michael Vineyard; Alexander Vlassov; Daniel Watts; Lawrence Weinstein; Dennis Weygand; M. Williams; Elliott Wolin; Michael Wood; Amrit Yegneswaran; Lorenzo Zana; Jixie Zhang; Bo Zhao; Zhiwen Zhao

    2007-11-01

    The cross section and decay angular distributions for the coherent \\phi meson photoproduction on the deuteron have been measured for the first time up to a squared four-momentum transfer t =(p_{\\gamma}-p_{\\phi})^2 =-2 GeV^2/c^2, using the CLAS detector at the Thomas Jefferson National Accelerator Facility. The cross sections are compared with predictions from a re-scattering model. In a framework of vector meson dominance, the data are consistent with the total \\phi-N cross section \\sigma_{\\phi N} at about 10 mb. If vector meson dominance is violated, a larger \\sigma_{\\phi N} is possible by introducing larger t-slope for the \\phi N \\to \\phi N process than that for the \\gamma N \\to \\phi N process. The decay angular distributions of the \\phi are consistent with helicity conservation.

  15. Certain tryptophan photoproducts are inhibitors of cytochrome P450-dependent mutagenicity

    SciTech Connect

    Rannug, U.; Agurell, E.; Cederberg, H. ); Rannug, A. )

    1992-01-01

    Two photoproducts, derived from UV-irradiation of the amino acid L-tryptophan and with high Ah (TCDD) receptor binding affinity, were tested for genotoxic and antimutagenic effects. The two indolo[3,2-b]carbazole derivatives, with the molecular weights of 284 and 312, respectively, were tested in Saccharomyces cerevisiae strain D7 for mitotic gene conversion and reverse mutation and in strain RS112 for sister chromatid conversion and gene conversion. No significant (P > 0.05) genotoxic effects were found in strain D7, while strain RS112 showed a small but significant increase in the frequency of sister chromatid conversions. In Chinese hamster ovary (CHO) cells the two compounds induced a statistically significant but less than twofold increase in the frequency of sister chromatid exchanges (SCE). No mutations were detected when the compounds were tested in Salmonella tphimurium strains TA98 and TA100. However, both 284 and 312 acted as antimutagens on strain TA100+S9 in the presence of benzo(a)pyrene. The decrease in mutagenicity by the most potent compound 284 was 20 revertants/nmol. This effect could be explained by an inhibitory effect on the cytochrome P450-dependent ethoxyresorufin O-deethylase (EROD) activity as seen in rat hepatocytes. The two compounds were also tested with hamster cells expressing rat cytochrome P-4501A1. The results support the conclusion that this cytochrome P-450 isozyme is inhibited by the tryptophan photoproducts. Similar results were also seen with two other high affinity Ah receptor ligands the quinazolinocarboline alkaloids rutaecapine and dehydrorutaecarpine. 20 refs., 3 figs., 4 tabs.

  16. Photoproduction of Scalar Mesons Using the CEBAF Large Acceptance Spectrometer (CLAS)

    NASA Astrophysics Data System (ADS)

    Chandavar, Shloka K.

    The search for glueballs has been ongoing for several decades. The lightest glueball has been predicted by quenched lattice QCD to have mass in the range of 1.0--1.7 GeV and JPC = 0++ . The mixing of glueball states with neighbouring meson states complicates their identification and hence several experiments have been carried out over the years to study the glueball candidates. By analyzing the decay channels and production mechanisms of these candidates, their glueball content can theoretically be determined. In reality, a lot of confusion still exists about the status of these glueball candidates. The f0(1500) is one of several contenders for the lightest glueball, which has been extensively studied in several different kinds of experiments. However, there exists no photoproduction data on this particle. In the analysis presented in this dissertation, the presence of the f0(1500) in the KS 0KS0 channel is investigated in photoproduction using the CEBAF Large Acceptance Spectrometer (CLAS) at the Thomas Jefferson National Accelerator Facility, also called Jefferson Lab (JLab). This is done by studying the reaction, gammap → fJp → KS0 KS0p → 2(pi +pi-)p using data from the g12 experiment. A clear peak is seen at 1500 MeV in the background subtracted data. This is enhanced if the momentum transfer is restricted to be less than 1 GeV2. Comparing with simulations, it is seen that this peak is associated with t channel production mechanism. The f 2'(1525) has a mass of 1525 MeV and a width of 73 MeV, and hence there is a possibility of it contributing to the peak observed in our data. A moments analysis seems to suggest some presence of a D wave, however, the low acceptance at forward and backward angles prohibits a definitive conclusion.

  17. Photoproduction of Hydrogen by Sulfur-Deprived Chlamydomonas reinhardtii Mutants with Impaired Photosystem II Photochemical Activity

    SciTech Connect

    Makarova, V. V.; Kosourov, S.; Krendeleva, T. E.; Semin, B. K.; Kukarskikh, G. P.; Rubin, A. B.; Sayre, R. T.; Ghirardi, M. L.; Seibert, M.

    2007-01-01

    Photoproduction of H2 was examined in a series of sulfur-deprived Chlamydomonas reinhardtii D1-R323 mutants with progressively impaired PSII photochemical activity. In the R323H, R323D, and R323E D1 mutants, replacement of arginine affects photosystem II (PSII) function, as demonstrated by progressive decreases in O2-evolving activity and loss of PSII photochemical activity. Significant changes in PSII activity were found when the arginine residue was replaced by negatively charged amino acid residues (R323D and R323E). However, the R323H (positively charged or neutral, depending on the ambient pH) mutant had minimal changes in PSII activity. The R323H, R323D, and R323E mutants and the pseudo-wild-type (pWt) with restored PSII function were used to study the effects of sulfur deprivation on H2-production activity. All of these mutants exhibited significant changes in the normal parameters associated with the H2-photoproduction process, such as a shorter aerobic phase, lower accumulation of starch, a prolonged anaerobic phase observed before the onset of H2-production, a shorter duration of H2-production, lower H2 yields compared to the pWt control, and slightly higher production of dark fermentation products such as acetate and formate. The more compromised the PSII photochemical activity, the more dramatic was the effect of sulfur deprivation on the H2-production process, which depends both on the presence of residual PSII activity and the amount of stored starch.

  18. The Enzyme-Mediated Direct Reversal of a Dithymine Photoproduct in Germinating Endospores

    PubMed Central

    Yang, Linlin; Li, Lei

    2013-01-01

    Spore photoproduct lyase (SPL) repairs a special thymine dimer, 5-thyminyl-5,6-dihydrothymine, which is commonly called spore photoproduct, or SP, in germinating endospores. SP is the exclusive DNA photo-damaging product found in endospores; its generation and swift repair by SPL are responsible for the spores’ extremely high UV resistance. Early in vivo studies suggested that SPL utilizes a direct reversal strategy to repair SP in the absence of light. Recently, it has been established that SPL belongs to the radical S-adenosylmethionine (SAM) superfamily. The enzymes in this superfamily utilize a tri-cysteine CXXXCXXC motif to bind a [4Fe-4S] cluster. The cluster provides an electron to the S-adenosylmethionine (SAM) to reductively cleave its C5′-S bond, generating a reactive 5′-deoxyadenosyl (5′-dA) radical. This 5′-dA radical abstracts the proR hydrogen atom from the C6 carbon of SP to initiate the repair process; the resulting SP radical subsequently fragments to generate a putative thymine methyl radical, which accepts a back-donated H atom to yield the repaired TpT. The H atom donor is suggested to be a conserved cysteine141 in B. subtilis SPL; the resulting thiyl radical likely interacts with a neighboring tyrosine99 before oxidizing the 5′-dA to 5′-dA radical and, subsequently, regenerating SAM. These findings suggest SPL to be the first enzyme in the large radical SAM superfamily (>44,000 members) to utilize a radical transfer pathway for catalysis; its study should shed light on the mechanistic understanding of the SAM regeneration process in other members of the superfamily. PMID:23799365

  19. Photoproduction of hydrogen by sulfur-deprived C. reinhardtii mutants with impaired photosystem II photochemical activity.

    PubMed

    Makarova, Valeria V; Kosourov, Sergey; Krendeleva, Tatiana E; Semin, Boris K; Kukarskikh, Galina P; Rubin, Andrei B; Sayre, Richard T; Ghirardi, Maria L; Seibert, Michael

    2007-10-01

    Photoproduction of H2 was examined in a series of sulfur-deprived Chlamydomonas reinhardtii D1-R323 mutants with progressively impaired PSII photochemical activity. In the R323H, R323D, and R323E D1 mutants, replacement of arginine affects photosystem II (PSII) function, as demonstrated by progressive decreases in O2-evolving activity and loss of PSII photochemical activity. Significant changes in PSII activity were found when the arginine residue was replaced by negatively charged amino acid residues (R323D and R323E). However, the R323H (positively charged or neutral, depending on the ambient pH) mutant had minimal changes in PSII activity. The R323H, R323D, and R323E mutants and the pseudo-wild-type (pWt) with restored PSII function were used to study the effects of sulfur deprivation on H2-production activity. All of these mutants exhibited significant changes in the normal parameters associated with the H2-photoproduction process, such as a shorter aerobic phase, lower accumulation of starch, a prolonged anaerobic phase observed before the onset of H2-production, a shorter duration of H2-production, lower H2 yields compared to the pWt control, and slightly higher production of dark fermentation products such as acetate and formate. The more compromised the PSII photochemical activity, the more dramatic was the effect of sulfur deprivation on the H2-production process, which depends both on the presence of residual PSII activity and the amount of stored starch. PMID:17701084

  20. Thymine photoproduct formation and inactivation of intact spores of Bacillus subtilis irradiated with short wavelength UV (200-300nm) at atmospheric pressure and in vacuo

    NASA Astrophysics Data System (ADS)

    Lindberg, C.; Horneck, G.

    Vacuum exposure renders the survival of spores of Bacillus subtilis approximately five times more sensitive to ultraviolet light irradiation than exposure under atmospheric conditions. The photoproduct formation in spores irradiated under ultrahigh vacuum (UHV) conditions is compared to the photoproduct formation in spores irradiated at atmospheric pressure. Compared to irradiation at atmospheric pressure, where only the ``spore photoproduct'' 5-thyminyl-5,6-dihydrothymine (TDHT) can be detected, two additional photoproducts, known as the c,s and t,s isomers of thymine dimer (T???T) are produced in vacuo. The spectral efficiencies for photoproduct formation in spores under atmospheric and vacuum conditions are compared. Since there is no increased formation of TDHT after irradiation in vacuum, TDHT cannot be made responsible for the observed vacuum effect. ``Vacuum specific'' photoproducts may cause a synergistic response of spores to the simultaneous action of ultraviolet light (UV) and UHV. Three different mechanisms are discussed for the enhanced sensitivity of B. subtilis spores to UV radiation in vacuum. The experiments described contribute valuable research information on the chance for survival of microorganisms in outer space.

  1. A TeGM6-4r antigen-based immunochromatographic test (ICT) for animal trypanosomosis.

    PubMed

    Nguyen, Thu-Thuy; Ruttayaporn, Ngasaman; Goto, Yasuyuki; Kawazu, Shin-ichiro; Sakurai, Tatsuya; Inoue, Noboru

    2015-11-01

    Animal trypanosomosis is a disease that is distributed worldwide which results in huge economic losses due to reduced animal productivity. Endemic regions are often located in the countryside where laboratory diagnosis is costly or inaccessible. The establishment of simple, effective, and accurate field tests is therefore of great interest to the farming and veterinary sectors. Our study aimed to develop a simple, rapid, and sensitive immunochromatographic test (ICT) for animal trypanosomosis utilizing the recombinant tandem repeat antigen TeGM6-4r, which is conserved amongst salivarian trypanosome species. In the specificity analysis, TeGM6-4r/ICT detected all of Trypanosoma evansi-positive controls from experimentally infected water buffaloes. As expected, uninfected controls tested negative. All sera samples collected from Tanzanian and Ugandan cattle that were Trypanosoma congolense- and/or Trypanosoma vivax-positive by microscopic examination of the buffy coat were found to be positive by the newly developed TeGM6-4r/ICT, which was comparable to results from TeGM6-4r/ELISA (kappa coefficient [κ] = 0.78). TeGM6/ICT also showed substantial agreement with ELISA using Trypanosoma brucei brucei (κ = 0.64) and T. congolense (κ = 0.72) crude antigen, suggesting the high potential of TeGM6-4r/ICT as a field diagnostic test, both for research purposes and on-site diagnosis of animal trypanosomosis. PMID:26290217

  2. Polarization observables in the longitudinal basis for pseudo-scalar meson photoproduction using a density matrix approach

    SciTech Connect

    Biplab Dey, Michael E. McCracken, David G. Ireland, Curtis A. Meyer

    2011-05-01

    The complete expression for the intensity in pseudo-scalar meson photoproduction with a polarized beam, target, and recoil baryon is derived using a density matrix approach that offers great economy of notation. A Cartesian basis with spins for all particles quantized along a single direction, the longitudinal beam direction, is used for consistency and clarity in interpretation. A single spin-quantization axis for all particles enables the amplitudes to be written in a manifestly covariant fashion with simple relations to those of the well-known CGLN formalism. Possible sign discrepancies between theoretical amplitude-level expressions and experimentally measurable intensity profiles are dealt with carefully. Our motivation is to provide a coherent framework for coupled-channel partial-wave analysis of several meson photoproduction reactions, incorporating recently published and forthcoming polarization data from Jefferson Lab.

  3. Upper limits for the photoproduction cross section for the Φ⁻⁻(1860) pentaquark state off the deuteron

    SciTech Connect

    Egiyan, H.; Langheinrich, J.; Gothe, R. W.; Graham, L.; Holtrop, M.; Lu, H.; Mattione, P.; Mutchler, G.; Park, K.; Smith, E. S.; Stepanyan, S.; Zhao, Z. W.; Adhikari, K. P.; Aghasyan, M.; Anghinolfi, M.; Baghdasaryan, H.; Ball, J.; Baltzell, N. A.; Battaglieri, M.; Bedlinskiy, I.; Bennett, R. P.; Biselli, A. S.; Bookwalter, C.; Branford, D.; Briscoe, W. J.; Brooks, W. K.; Burkert, V. D.; Carman, D. S.; Celentano, A.; Chandavar, S.; Contalbrigo, M.; D’Angelo, A.; Daniel, A.; Dashyan, N.; De Vita, R.; De Sanctis, E.; Deur, A.; Dey, B.; Dickson, R.; Djalali, C.; Doughty, D.; Dupre, R.; El Alaoui, A.; El Fassi, L.; Eugenio, P.; Fedotov, G.; Fegan, S.; Fradi, A.; Gabrielyan, M. Y.; Gevorgyan, N.; Gilfoyle, G. P.; Giovanetti, K. L.; Girod, F. X.; Goetz, J. T.; Gohn, W.; Golovatch, E.; Griffioen, K. A.; Guidal, M.; Guler, N.; Guo, L.; Gyurjyan, V.; Hafidi, K.; Hakobyan, H.; Hanretty, C.; Heddle, D.; Hicks, K.; Ilieva, Y.; Ireland, D. G.; Ishkhanov, B. S.; Jo, H. S.; Joo, K.; Khetarpal, P.; Kim, A.; Kim, W.; Klein, A.; Klein, F. J.; Kubarovsky, V.; Kuleshov, S. V.; Livingston, K.; MacGregor, I. J. D.; Mao, Y.; Mayer, M.; McKinnon, B.; Mokeev, V.; Munevar, E.; Nadel-Turonski, P.; Ni, A.; Niculescu, G.; Ostrovidov, A. I.; Paolone, M.; Pappalardo, L.; Paremuzyan, R.; Park, S.; Pasyuk, E.; Anefalos Pereira, S.; Phelps, E.; Pogorelko, O.; Pozdniakov, S.; Price, J. W.; Procureur, S.; Protopopescu, D.; Raue, B. A.; Ricco, G.; Rimal, D.; Ripani, M.; Ritchie, B. G.; Rosner, G.; Rossi, P.; Sabatié, F.; Saini, M. S.; Salgado, C.; Schott, D.; Schumacher, R. A.; Seder, E.; Seraydaryan, H.; Sharabian, Y. G.; Smith, G. D.; Sober, D. I.; Stepanyan, S. S.; Strauch, S.; Taiuti, M.; Tang, W.; Taylor, C. E.; Tedeschi, D. J.; Ungaro, M.; Voutier, E.; Watts, D. P.; Weinstein, L. B.; Weygand, D. P.; Wood, M. H.; Zachariou, N.; Zana, L.; Zhao, B.

    2012-01-30

    We searched for the Φ⁻⁻(1860) pentaquark in the photoproduction process off the deuteron in the Ξ⁻π⁻-decay channel using CLAS. The invariant-mass spectrum of the Ξ⁻π⁻ system does not indicate any statistically significant enhancement near the reported mass M=1.860 GeV. The statistical analysis of the sideband-subtracted mass spectrum yields a 90%-confidence-level upper limit of 0.7 nb for the photoproduction cross section of Φ⁻⁻(1860) with a consecutive decay intoΞ⁻π⁻ in the photon-energy range 4.5GeVγ<5.5GeV.

  4. Upper limits for the photoproduction cross section for the Φ⁻⁻(1860) pentaquark state off the deuteron

    DOE PAGESBeta

    Egiyan, H.; Langheinrich, J.; Gothe, R. W.; Graham, L.; Holtrop, M.; Lu, H.; Mattione, P.; Mutchler, G.; Park, K.; Smith, E. S.; et al

    2012-01-30

    We searched for the Φ⁻⁻(1860) pentaquark in the photoproduction process off the deuteron in the Ξ⁻π⁻-decay channel using CLAS. The invariant-mass spectrum of the Ξ⁻π⁻ system does not indicate any statistically significant enhancement near the reported mass M=1.860 GeV. The statistical analysis of the sideband-subtracted mass spectrum yields a 90%-confidence-level upper limit of 0.7 nb for the photoproduction cross section of Φ⁻⁻(1860) with a consecutive decay intoΞ⁻π⁻ in the photon-energy range 4.5GeVγ<5.5GeV.

  5. A fresh look at factorization breaking in diffractive photoproduction of dijets at HERA at next-to-leading order QCD

    NASA Astrophysics Data System (ADS)

    Guzey, V.; Klasen, M.

    2016-08-01

    We calculate the cross section of diffractive dijet photoproduction in ep scattering at next-to-leading order (NLO) of perturbative QCD (pQCD), which we supplement by a model of factorization breaking for the resolved-photon contribution. In this model, the suppression depends on the flavor and momentum fraction of the partons in the photon. We show that within experimental and theoretical uncertainties, the resulting approach provides a good description of the available HERA data in most of the bins. Hence, taken together with the observation that NLO pQCD explains well the data on diffractive photoproduction of open charm in ep scattering, our model of factorization breaking presents a viable alternative to the scheme based on the global suppression factor.

  6. Diagnostic value of the recombinant tandem repeat antigen TeGM6-4r for surra in water buffaloes.

    PubMed

    Nguyen, Thu-Thuy; Zhou, Mo; Ruttayaporn, Ngasaman; Nguyen, Quoc Doanh; Nguyen, Viet Khong; Goto, Yasuyuki; Suzuki, Yasuhiko; Kawazu, Shin-ichiro; Inoue, Noboru

    2014-03-17

    Trypanosoma evansi infection, or surra, is currently affecting various species of animals, especially water buffaloes. Since diagnosis is an important aspect of surra control, development of novel diagnostic antigens is of interest to implement and improve the currently utilized methods. Our study evaluated the tandem repeat antigen TeGM6-4r in T. evansi antibody detection in water buffaloes. TeGM6-4r-based ELISA was performed with 20 positive and 8 negative controls and 484 field samples from water buffaloes in Northern Vietnam. To examine cross-reactivity, sera from Japanese cattle that had been experimentally infected with Theileria orientalis (n=10), Babesia bovis (n=3), Babesia bigemina (n=7) and Trypanosoma theileri (n=59) were included in the study. The sensitivity of the test was 80%. TeGM6-4r did not react with Theileria or Babesia infected sera, however it showed cross reactivity with 11/59 T. theileri infected samples. The reference test, CATT/T. evansi also reacted with 3/59 T. theileri infected sera. The lysate antigen-based ELISA reacted with 4/59 T. theileri, 9/10 Theileria and 3/10 Babesia infected sera. In contrast, TeGM6-4r-based ELISA was 86.3% sensitive and 58.3% specific in the screening of field samples. The average seroprevalence of T. evansi infection among water buffaloes in Northern Vietnam was 27.1% by CATT/T. evansi and 53.7% by TeGM6-4r. Seroprevalence in the five surveyed provinces ranged from 17.4% to 39.8% in the reference test, and 47.3% to 67.3% in the recombinant antigen based test. The finding indicated that the disease is still widely endemic in the area and that surveillance programs need to be carried out regularly to better control surra. We proposed TeGM6-4r as a useful serodiagnostic antigen for the detection and epidemiological surveillance of T. evansi infection among water buffaloes.

  7. Irreducible tensor approach to spin observables in the photoproduction of mesons with arbitrary spin-parity s{sup {pi}}

    SciTech Connect

    Ramachandran, G.; Vidya, M. S.; Balasubramanyam, J.

    2007-06-15

    A theoretical formalism leading to elegant derivation of formulas for all spin observables is outlined for photoproduction of mesons with arbitrary spin-parity s{sup {pi}}. The salient features of this formalism, based on irreducible tensor techniques, are (i) the number of independent irreducible tensor amplitudes is 4(2s+1) (ii) a single compact formula is sufficient to express these amplitudes in terms of allowed electric and magnetic multipole amplitudes, and (iii) all the spin observables, including beam analyzing powers as well as the differential cross section, are expressible in terms of bilinear irreducible tensors of rank 0 to 2(s+1). The relationship between the irreducible tensor amplitudes and the helicity amplitudes is elucidated in general and explicit expressions for the helicity amplitudes are given in terms of the irreducible tensor amplitudes in the particular cases of pseudoscalar and vector meson photoproduction. The connection between the irreducible tensor amplitudes introduced here and the well-known Chew-Goldberger-Low-Nambu amplitudes for photoproduction of pseudoscalar mesons is also established.

  8. Adenine photodimerization in deoxyadenylate sequences: elucidation of the mechanism through structural studies of a major d(ApA) photoproduct.

    PubMed Central

    Kumar, S; Joshi, P C; Sharma, N D; Bose, S N; Jeremy, R; Davies, H; Takeda, N; McCloskey, J A

    1991-01-01

    The mechanism of the photodimerization of adjacent adenine bases on the same strand of DNA has been elucidated by determining the structure of one of the two major photoproducts that are formed by UV irradiation of the deoxydinucleoside monophosphate d(ApA). The photoproduct, denoted d(ApA)*, corresponds to a species of adenine photodimer first described by Pörschke (Pörschke, D. (1973) J.Am.Chem.Soc. 95, 8440-8446). From a detailed examination of its chemical and spectroscopic properties, including comparisons with the model compound N-cyano-N1-(1-methylimidazol-5-yl)formamidine, it is deduced that d(ApA)* contains a deoxyadenosine unit covalently linked through its C(8) position to C(4) of an imidazole N(1) deoxyribonucleoside moiety bearing an N-cyanoformamidino substituent at C(5). On treatment with acid, d(ApA)* is degraded with high specificity to 8-(5-amino-imidazol-4-yl)adenine whose identity has been confirmed by independent chemical synthesis. It is concluded that the primary event in adenine photodimerization entails photoaddition of the N(7)-C(8) double bond of the 5'-adenine across the C(6) and C(5) positions of the 3'-adenine. The azetidine species thus generated acts as a common precursor to both types of d(ApA) photoproduct which are formed from it by competing modes of azetidine ring fission. PMID:2057348

  9. Misinsertion and bypass of thymine-thymine dimers by human DNA polymerase iota.

    PubMed

    Tissier, A; Frank, E G; McDonald, J P; Iwai, S; Hanaoka, F; Woodgate, R

    2000-10-01

    Human DNA polymerase iota (pol(iota)) is a recently discovered enzyme that exhibits extremely low fidelity on undamaged DNA templates. Here, we show that poliota is able to facilitate limited translesion replication of a thymine-thymine cyclobutane pyrimidine dimer (CPD). More importantly, however, the bypass event is highly erroneous. Gel kinetic assays reveal that pol(iota) misinserts T or G opposite the 3' T of the CPD approximately 1.5 times more frequently than the correct base, A. While pol(iota) is unable to extend the T.T mispair significantly, the G.T mispair is extended and the lesion completely bypassed, with the same efficiency as that of the correctly paired A. T base pair. By comparison, pol(iota) readily misinserts two bases opposite a 6-4 thymine-thymine pyrimidine-pyrimidone photoproduct (6-4PP), but complete lesion bypass is only a fraction of that observed with the CPD. Our data indicate, therefore, that poliota possesses the ability to insert nucleotides opposite UV photoproducts as well as to perform unassisted translesion replication that is likely to be highly mutagenic.

  10. Discovery of a 6.4 keV Emission Line in a Burst from SGR 1900+14

    NASA Technical Reports Server (NTRS)

    Strohmayer, Tod E.; Ibrahim, Alaa I.

    2000-01-01

    We present evidence of a 6.4 key emission line during a burst from the soft gamma repeater SGR 1900+14. The Rossi X-Ray Timing Explorer (RXTE) monitored this source extensively during its outburst in the summer of 1998. A strong burst observed on 1998 August 29 revealed a number of unique properties. The burst exhibits a precursor and is followed by a long (approx. 10(exp 3) s) tail modulated at the 5.16 s stellar rotation period. The precursor has a duration of approx. equals 0.85 s and shows both significant spectral evolution as well as an emission feature centered near 6.4 keV during the first 0.3 s of the event, when the X-ray spectrum was hardest. The continuum during the burst is well fit with an optically thin thermal bremsstrahlung spectrum with the temperature ranging from approx. equals 40 to 10 keV. The line is strong, with an equivalent width of approx. 400 eV, and is consistent with Fe K(alpha) fluorescence from relatively coot material. If the rest-frame energy is indeed 6.4 keV, then the lack of an observed redshift indicates that the source is at least approx. 80 km above the neutron star surface. We discuss the implications of the line detection in the context of models for SGRs.

  11. Exclusive Photoproduction of Charged Pions in Hydrogen and Deuterium from 1 to 6 GeV

    SciTech Connect

    Lingyan Zhu

    2004-02-28

    The study of the transition region in the description of exclusive processes and hadron structure, from the nucleon-meson degrees of freedom in meson-exchange models at low energy to the quark-gluon degrees of freedom in pQCD at high energy, is essential for us to understand the strong interaction. The differential cross section measurements for exclusive reactions at fixed center-of-mass angles enable us to investigate the constituent counting rule, which explicitly connects the quark-gluon degrees of freedom to the energy dependence of differential cross sections. JLab Experiment E94-104 was carried out in Hall A with two high resolution spectrometers. It included the coincidence cross section measurement for the [gamma]n --> pi{sup -}[p] process with a deuterium target and the singles measurement for the [gamma]p --> pi{sup +}[n] process with a hydrogen target. The untagged real photons were generated by the electron beam impinging on a copper radiator. The photon energies ranged from 1.1 to 5.5 GeV, corresponding to the center-of-mass energies from 1.7 to 3.4 GeV. The pion center-of-mass angles were fixed at 50 deg, 70 deg, 90 deg, and also 100 deg, 110 deg at a few energies. The JLab E94-104 data presented in this thesis contain four interesting features. The data exhibit a global scaling behavior for both [pi]{sup -} and [pi]{sup +} photoproduction at high energies and high transverse momenta, consistent with the constituent counting rule and the existing [pi]{sup +} photoproduction data. This implies that the quark-gluon degrees of freedom start to play a role at this energy scale. The data suggests possible substructure of the scaling behavior, which might be oscillations around the scaling value. There are several possible mechanisms that can cause oscillations, for example the one associated with the generalized constituent counting rule involving quark orbital angular momentum. The data show an enhancement in the scaled cross section at center

  12. Mechanism elucidation of the radical SAM enzyme spore photoproduct lyase (SPL)

    PubMed Central

    Li, Lei

    2011-01-01

    Spore photoproduct lyase (SPL) repairs a special thymine dimer 5-thyminyl-5,6-dihydrothymine, which is commonly called spore photoproduct or SP at the bacterial early germination phase. SP is the exclusive DNA photo-damage product in bacterial endospores; its generation and swift repair by SPL are responsible for the spores’ extremely high UV resistance. The early in vivo studies suggested that SPL utilizes a direct reversal strategy to repair the SP in the absence of light. The research in the past decade further established SPL as a radical SAM enzyme, which utilizes a tri-cysteine CXXXCXXC motif to harbor a [4Fe-4S] cluster. At the 1+ oxidation state, the cluster provides an electron to the S-adenosylmethionine (SAM), which binds to the cluster in a bidentate manner as the fourth and fifth ligands, to reductively cleave the C-S bond associated with the sulfonium ion in SAM, generating a reactive 5′-deoxyadenosyl (5′-dA) radical. This 5′-dA radical abstracts the proR hydrogen atom from the C6 carbon of SP to initiate the repair process; the resulting SP radical subsequently fragments to generate a putative thymine methyl radical, which accepts a back-donated H atom to yield the repaired TpT. SAM is suggested to be regenerated at the end of each catalytic cycle; and only a catalytic amount of SAM is needed in the SPL reaction. The H atom source for the back donation step is suggested to be a cysteine residue (C141 in B. subtilis SPL), and the H-atom transfer reaction leaves a thiyl radical behind on the protein. This thiyl radical thus must participate in the SAM regeneration process; however how the thiyl radical abstracts an H atom from the 5′-dA to regenerate SAM is unknown. This paper reviews and discusses the history and the latest progress in the mechanistic elucidation of SPL. Despite some recent breakthroughs, more questions are raised in the mechanistic understanding of this intriguing DNA repair enzyme. PMID:22197590

  13. Mechanistic studies of the spore photoproduct lyase via a single cysteine mutation.

    PubMed

    Yang, Linlin; Lin, Gengjie; Nelson, Renae S; Jian, Yajun; Telser, Joshua; Li, Lei

    2012-09-11

    5-Thyminyl-5,6-dihydrothymine (also called spore photoproduct or SP) is the exclusive DNA photodamage product in bacterial endospores. It is repaired by a radical SAM (S-adenosylmethionine) enzyme, the spore photoproduct lyase (SPL), at the bacterial early germination phase. Our previous studies proved that SPL utilizes the 5'-dA• generated by the SAM cleavage reaction to abstract the H(6proR) atom to initiate the SP repair process. The resulting thymine allylic radical was suggested to take an H atom from an unknown protein source, most likely cysteine 141. Here we show that C141 can be readily alkylated in the native SPL by an iodoacetamide treatment, suggesting that it is accessible to the TpT radical. SP repair by the SPL C141A mutant yields TpTSO(2)(-) and TpT simultaneously from the very beginning of the reaction; no lag phase is observed for TpTSO(2)(-) formation. Should any other protein residue serve as the H donor, its presence would result in TpT being the major product at least for the first enzyme turnover. These observations provide strong evidence to support C141 as the direct H atom donor. Moreover, because of the lack of this intrinsic H donor, the C141A mutant produces TpT via an unprecedented thymine cation radical reduction (proton-coupled electron transfer) process, contrasting to the H atom transfer mechanism in the wild-type (WT) SPL reaction. The C141A mutant repairs SP at a rate that is ~3-fold slower than that of the WT enzyme. Formation of TpTSO(2)(-) and TpT exhibits a V(max) deuterium kinetic isotope effect (KIE) of 1.7 ± 0.2, which is smaller than the (D)V(max) KIE of 2.8 ± 0.3 determined for the WT SPL reaction. These findings suggest that removing the intrinsic H atom donor disturbs the rate-limiting process during enzyme catalysis. As expected, the prereduced C141A mutant supports only ~0.4 turnover, which is in sharp contrast to the >5 turnovers exhibited by the WT SPL reaction, suggesting that the enzyme catalytic cycle (SAM

  14. Mechanisms for selective toxicity of fipronil insecticide and its sulfone metabolite and desulfinyl photoproduct.

    PubMed

    Hainzl, D; Cole, L M; Casida, J E

    1998-12-01

    Fipronil, an N-phenylpyrazole with a trifluoromethylsulfinyl substituent, initiated the second generation of insecticides acting at the gamma-aminobutyric acid (GABA) receptor to block the chloride channel. The first generation includes the polychlorocycloalkanes alpha-endosulfan and lindane. In this study, we examine the mechanisms for selective toxicity of the sulfoxide fipronil and its sulfone metabolite and desulfinyl photoproduct relative to their target site interactions in vitro and ex vivo and the importance in fipronil action of biooxidation to the sulfone. Differences in GABA receptor sensitivity, assayed by displacement of 4'-ethynyl-4-n-[2, 3-3H2]propylbicycloorthobenzoate ([3H]EBOB) from the noncompetitive blocker site, appear to be a major factor in fipronil being much more toxic to the insects (housefly and fruit fly) than to the vertebrates (humans, dogs, mice, chickens, quail, and salmon) examined; in insects, the IC50s range from 3 to 12 nM for fipronil and its sulfone and desulfinyl derivatives, while in vertebrates, the IC50 average values are 1103, 175, and 129 nM for fipronil, fipronil sulfone, and desulfinyl fipronil, respectively. The insect relative to the vertebrate specificity decreases in the following order: fipronil > lindane > desulfinyl fipronil > fipronil sulfone > alpha-endosulfan. Ex vivo inhibition of [3H]EBOB binding in mouse brain is similar for fipronil and its sulfone and desulfinyl derivatives at the LD50 dose, but surprisingly, at higher doses fipronil can be lethal without detectably blocking the [3H]EBOB site. The P450 inhibitor piperonyl butoxide, acting in houseflies, increases the metabolic stability and effectiveness of fipronil and the sulfone but not those of the desulfinyl compound, and in mice it completely blocks the sulfoxide to sulfone conversion without altering the poisoning. Thus, the selective toxicity of fipronil and fipronil-derived residues is due in part to the higher potency of the parent compound at

  15. Genotoxicity of sulcotrione pesticide and photoproducts on Allium cepa root meristem.

    PubMed

    Goujon, Eric; Sta, Chaima; Trivella, Aurélien; Goupil, Pascale; Richard, Claire; Ledoigt, Gérard

    2014-07-01

    Contamination by toxic agents in the environment has become matters of concern to agricultural countries. Sulcotrione, a triketone herbicide used to control dicotyledonous weeds in maize culture is rapidly photolyzed on plant foliage and generate two main photoproducts the xanthene-1,9-dione-3,4-dihydro-6-methylsulfonyl and 2-chloro-4-mesylbenzoic acid (CMBA). The aim of this study was to analyze the potential toxicity of the herbicide and the irradiated herbicide cocktail. Cytotoxicity and genotoxicity of non irradiated and irradiated sulcotrione were investigated in Allium cepa test. The sulcotrione irradiation was monitored under sunlight simulated conditions to reach 50% of phototransformation. Concentrations of sulcotrione in the range 5 × 10(-)(9)-5 × 10(-)(5)M were tested. Cytological analysis of root tips cells showed that both non irradiated and irradiated sulcotrione caused a dose-dependent decrease of mitotic index with higher cytotoxicity for the irradiated herbicide which can lead to 24.2% reduction of mitotic index compared to water control. Concomitantly, chromosomal aberrations were observed in A.cepa root meristems. Both non irradiated sulcotrione and irradiated sulcotrione induced a dose-dependent increase of chromosomal abnormalities frequencies to a maximal value of 33.7%. A saturating effect in anomaly frequencies was observed in meristems treated with high concentrations of non irradiated sulcotrione only. These data suggest that photolyzed sulcotrione cocktail have a greater cytotoxicity and genotoxicity than parent molecule and question about the impact of photochemical process on environment. PMID:25052526

  16. DNA repair of a single UV photoproduct in a designed nucleosome

    SciTech Connect

    Kosmoskil, Joseph V.; Ackerman, Eric J. ); Smerdon, Michael J.

    2001-08-28

    Eukaryotic DNA repair enzymes must interact with the architectural hierarchy of chromatin. The challenge of finding damaged DNA complexed with histone proteins in nucleosomes is complicated by the need to maintain local chromatin structures involved in regulating other DNA processing events. The heterogeneity of lesions induced by DNA-damaging agents has led us to design homogeneously damaged substrates to directly compare repair of naked DNA with that of nucleosomes. Here we report that nucleotide excision repair in Xenopus nuclear extracts can effectively repair a single UV radiation photoproduct located 5 bases from the dyad center of a positioned nucleosome, although the nucleosome is repaired at about half the rate at which the naked DNA fragment is. Extract repair within the nucleosome is > 50-fold more rapid than either enzymatic photoreversal or endonuclease cleavage of the lesion in vitro. Furthermore, nucleosome formation occurs (after repair) only on damaged naked DNA ( 165-bp fragments) during a 1-h incubation in these extracts, even in the presence of a large excess of undamaged DNA. This is an example of selective nucleosome assembly by Xenopus nuclear extracts on a short linear DNA fragment containing a DNA lesion.

  17. Enhanced photoproduction of hydrogen peroxide by humic substances in the presence of phenol electron donors.

    PubMed

    Zhang, Yi; Simon, Kelli A; Andrew, Andrea A; Del Vecchio, Rossana; Blough, Neil V

    2014-11-01

    Addition of a series of phenol electron donors to solutions of humic substances (HS) enhanced substantially the initial rates of hydrogen peroxide (H2O2) photoproduction (RH2O2), with enhancement factors (EF) ranging from a low of ∼3 for 2,4,6-trimethylphenol (TMP) to a high of ∼15 for 3,4-dimethoxyphenol (DMOP). The substantial inhibition of the enhanced RH2O2 following borohydride reduction of the HS, as well as the dependence of RH2O2 on phenol and dioxygen concentrations are consistent with a mechanism in which the phenols react with the triplet excited states of (aromatic) ketones within the HS to form initially a phenoxy and ketyl radical. The ketyl radical then reacts rapidly with dioxygen to regenerate the ketone and form superoxide (O2-), which subsequently dismutates to H2O2. However, as was previously noted for the photosensitized loss of TMP, the incomplete inhibition of the enhanced RH2O2 following borohydride reduction suggests that there may remain another pool of oxidizing triplets. The results demonstrate that H2O2 can be generated through an additional pathway in the presence of sufficiently high concentrations of appropriate electron donors through reaction with the excited triplet states of aromatic ketones and possibly of other species such as quinones. However, in some cases, the much lower ratio of H2O2 produced to phenol consumed suggests that secondary reactions could alter this ratio significantly.

  18. Λ(1405) poles obtained from π0Σ0 photoproduction data

    NASA Astrophysics Data System (ADS)

    Roca, L.; Oset, E.

    2013-05-01

    We present a strategy to extract the position of the two Λ(1405) poles from experimental photoproduction data measured recently at different energies in the γp→K+π0Σ0 reaction at Jefferson Laboratory. By means of a chiral dynamics motivated potential with free parameters, we solve the Bethe-Salpeter equation in the coupled channels K¯N and πΣ in isospin I=0 and parametrize the amplitude for the photonuclear reaction in terms of a linear combination of the πΣ→πΣ and K¯N→πΣ scattering amplitudes in I=0, with a different linear combination for each energy. Good fits to the data are obtained with some sets of parameters, by means of which one can also predict the cross section for the K-p→π0Σ0 reaction. These later results help us decide among the possible solutions. The result is that the different solutions lead to two poles similar to those found in the chiral unitary approach. With the best result we find the two Λ(1405) poles at 1385-68iMeV and 1419-22iMeV.

  19. Search for Resonances in the Photoproduction of Proton-Antiproton Pairs

    SciTech Connect

    Stokes, Burnham

    2006-01-01

    Results are reported on the reaction γp → p$\\bar{p}$p with beam energy in the range 4.8-5.5 GeV. The data were collected at the Thomas Jefferson National Accelerator Facility in CLAS experiment E01-017(G6C). The focus of this study is an understanding of the mechanisms of photoproduction of proton-antiproton pairs, and to search for intermediate resonances, both narrow and broad, which decay to p$\\bar{p}$. The total measured cross section in the photon energy range 4.8-5.5 GeV is σ = 33 ± 2 nb. Measurement of the cross section as a function of energy is provided. An upper limit on the production of a narrow resonance state previously observed with a mass of 2.02 GeV/c2 is placed at 0.35 nb. No intermediate resonance states were observed. Meson exchange production appears to dominate the production of the proton-antiproton pairs.

  20. Photoproduction of J /ψ mesons in peripheral and semicentral heavy ion collisions

    NASA Astrophysics Data System (ADS)

    Kłusek-Gawenda, Mariola; Szczurek, Antoni

    2016-04-01

    We calculate total and differential cross sections for J /ψ photoproduction in ultrarelativistic lead-lead collisions at the LHC energy √{sN N}=2.76 TeV. In the present approach we use a simple model based on vector dominance picture and multiple scattering of the hadronic (c c ¯ ) state in a cold nucleus as an example. In our analysis we use both the classical mechanics and quantum (Glauber) formulas for calculating σtot ,J /ψ P b which is a building block of our model. We compare our UPC results with ALICE and CMS data. For semicentral collisions (b

  1. Fast Electrical Potential from a Long-Lived, Long-Wavelength Photoproduct of Fly Visual Pigment

    PubMed Central

    Pak, William L.; Lidington, Kellie J.

    1974-01-01

    A rapid electrical potential, which we have named the M-potential, can be obtained from the Drosophila eye using a high energy flash stimulus. The potential can be elicited from the normal fly, but it is especially prominent in the mutant norp AP12 (a phototransduction mutant), particularly if the eye color pigments are genetically removed from the eye. Several lines of evidence suggest that the M-potential arises from photoexcitation of long-lived metarhodopsin. Photoexcitation of rhodopsin does not produce a comparable potential. The spectral sensitivity of the M-potential peaks at about 575 nm. The M-potential pigment (metarhodopsin) can be shown to photoconvert back and forth with a "silent pigment(s)" absorbing maximally at about 485 nm. The silent pigment presumably is rhodopsin. These results support the recent spectrophotometric findings that dipteran metarhodopsin absorbs at much longer wavelengths than rhodopsin. The M-potential probably is related to the photoproduct component of the early receptor potential (ERP). Two major differences between the M-potential and the classical ERP are: (a) Drosophila rhodopsin does not produce a rapid photoresponse, and (b) an anesthetized or freshly sacrificed animal does not yield the M-potential. As in the case of the ERP, the M-potential appears to be a response associated with a particular state of the fly visual pigment. Therefore, it should be useful in in vivo investigations of the fly visual pigment, about which little is known. PMID:4829527

  2. Prompt photon photoproduction at HERA within the framework of the quark Reggeization hypothesis

    NASA Astrophysics Data System (ADS)

    Saleev, V. A.

    2008-12-01

    We study the inclusive production of isolated prompt photons within the framework of the quasi-multi-Regge-kinematic approach, applying the quark Reggeization hypothesis. We describe accurately and without free parameters the transverse momentum and pseudorapidity spectra of prompt photons in the inclusive photoproduction at the HERA Collider. It is shown that the main mechanism of the inclusive prompt photon production in the γp collisions is the fusion of a Reggeized quark (antiquark) from the proton and a collinear antiquark (quark) from the photon into a photon, via the effective Reggeon-quark-gamma vertex. The fragmentation of the quark, which is produced via the gamma-Reggeon-quark and quark-Reggeon-quark vertices, into a photon is strongly suppressed by the isolation cone condition and it gives a significant contribution in the region of a large negative pseudorapidity only. At the stage of numerical calculations we use the Kimber-Martin-Ryskin prescription for unintegrated quark and gluon distribution functions, with the following collinear parton densities as input: Martin-Roberts-Stirling-Thorne for a proton and Glück-Reya-Vogt for a photon.

  3. Photoproduction of the \\Theta^+ resonance on the nucleon in a Regge model

    SciTech Connect

    H. Kwee; M. Guidal; M. Polyakov; M. Vanderhaeghen

    2005-09-15

    We estimate the reaction mechanisms for the photoproduction of the {Theta}{sup +}(1540) resonance on the nucleon, through K and K* Regge exchanges. We compare the size of the cross sections for the {gamma}n {yields} K{sup -} {Theta}{sup +} and {gamma}p {yields} {bar K}{sup 0} {Theta}{sup +} reactions, and investigate their sensitivity to the spin-parity assignments J{sup P} = (1/2){sup {+-}}, (3/2){sup {+-}} for the {Theta}{sup +} resonance. The model allows to estimate the cross sections corresponding with a given upper bound on the width of the {Theta}{sup +}. Within this model, the cross sections on the neutron are found to be around a factor 5 larger than the ones on the proton, due to the presence of charged K exchange for the reaction on a neutron target. Furthermore, the photon asymmetry is found to display a pronounced sensitivity to the parity of the {Theta}{sup +}, making it a very promising observable to help determining the quantum numbers of the {Theta}{sup +} resonance.

  4. Carbon monoxide photoproduction: implications for photoreactivity of Arctic permafrost-derived soil dissolved organic matter.

    PubMed

    Hong, Jun; Xie, Huixiang; Guo, Laodong; Song, Guisheng

    2014-08-19

    Apparent quantum yields of carbon monoxide (CO) photoproduction (AQY(CO)) for permafrost-derived soil dissolved organic matter (SDOM) from the Yukon River Basin and Alaska coast were determined to examine the dependences of AQY(CO) on temperature, ionic strength, pH, and SDOM concentration. SDOM from different locations and soil depths all exhibited similar AQY(CO) spectra irrespective of soil age. AQY(CO) increased by 68% for a 20 °C warming, decreased by 25% from ionic strength 0 to 0.7 mol L(-1), and dropped by 25-38% from pH 4 to 8. These effects combined together could reduce AQY(CO) by up to 72% when SDOM transits from terrestrial environemnts to open-ocean conditions during summer in the Arctic. A Michaelis-Menten kinetics characterized the influence of SDOM dilution on AQY(CO) with a very low substrate half-saturation concentration. Generalized global-scale relationships between AQY(CO) and salinity and absorbance demostrate that the CO-based photoreactivity of ancient permaforst SDOM is comparable to that of modern riverine DOM and that the effects of the physicochemical variables revealed here alone could account for the seaward decline of AQY(CO) observed in diverse estuarine and coastal water bodies.

  5. Beam Asymmetries in {pi}{sup 0} Photoproduction on the Proton

    SciTech Connect

    Sparks, Nathan

    2010-08-05

    Polarization observables, such as the beam asymmetry {Sigma}, are vital to the study of the many N* and {Delta} resonances that decay to the p{pi}{sup 0} final state. Beam asymmetries in {pi}{sup 0} photoproduction off the proton have been determined using the Crystal Barrel CsI(Tl) calorimeter at ELSA, University of Bonn in Germany, in the energy range E{gamma} = 920 to 1680 MeV by analyzing the neutral decay mode {pi}{sup 0{yields}{gamma}{gamma}}. In this experiment, the BaF{sub 2} spectrometer TAPS was placed in the forward direction increasing the solid angle coverage to nearly 4{pi} and serving as a fast trigger. For the first time, these measurements include the most forward angles in {theta}{sub {pi}}{sup 0c{center_dot}m}. Additionally, this analysis provides new data points in the energy range E{gamma} = 1510 to 1680 MeV where few measurements have previously been made.

  6. Chemiexcitation of Melanin Derivatives Induces DNA Photoproducts Long after UV Exposure

    PubMed Central

    Premi, Sanjay; Wallisch, Silvia; Mano, Camila M.; Weiner, Adam B.; Bacchiocchi, Antonella; Wakamatsu, Kazumasa; Bechara, Etelvino J. H.; Halaban, Ruth; Douki, Thierry; Brash, Douglas E.

    2015-01-01

    Mutations in sunlight-induced melanoma arise from cyclobutane pyrimidine dimers (CPD), DNA photoproducts that are typically created picoseconds after an ultraviolet (UV) photon is absorbed at thymine or cytosine. Here we show that in melanocytes, CPD are generated for >3 hours after exposure to UVA, a major component of the radiation in sunlight and in tanning beds. These “dark CPD” constitute the majority of CPD and include the cytosine-containing CPD that initiate UV-signature C→T mutations. Dark CPD arise when UV-induced reactive oxygen and nitrogen species combine to excite an electron in fragments of the pigment melanin. This creates a quantum triplet state that has the energy of a UV photon but that induces CPD by energy transfer to DNA in a radiation-independent manner. Melanin may thus be carcinogenic as well as protective against cancer. These findings also validate the long-standing suggestion that chemically-generated excited electronic states are relevant to mammalian biology. PMID:25700512

  7. Analysis of recent CLAS data on Σ*(1385) photoproduction off a neutron target

    NASA Astrophysics Data System (ADS)

    Wang, Xiao-Yun; He, Jun; Haberzettl, Helmut

    2016-04-01

    Based on recent experimental data obtained by the CLAS Collaboration, the Σ (1385 ) photoproduction off a neutron target at laboratory photon energies Eγ up to 2.5 GeV is investigated in an effective Lagrangian approach including s -, u -, and t -channel Born-term contributions. The present calculation does not take into account any explicit s -channel baryon-resonance contributions; however, in the spirit of duality, we include t -channel exchanges of mesonic Regge trajectories. The onset of the Regge regime is controlled by smoothly interpolating between Feynman-type single-meson exchanges and full-fledged Regge-trajectory exchanges. Gauge invariance broken by the Regge treatment is fully restored by introducing contact-type interaction currents that result from the implementation of local gauge invariance in terms of generalized Ward-Takahashi identities. The cross sections for the γ n →K+Σ*(1385) - reaction are calculated and compared with experimental results from the CLAS and LEPS collaborations. Despite its simplicity, the present theoretical approach provides a good description of the main features of the data. However, the parameters fitted to the data show that the gauge-invariance-restoring contact term plays a large role, which may point to large contributions from final-state interactions.

  8. Photoproduction of π0 mesons off neutrons in the nucleon resonance region.

    PubMed

    Dieterle, M; Keshelashvili, I; Ahrens, J; Annand, J R M; Arends, H J; Bantawa, K; Bartolome, P A; Beck, R; Bekrenev, V; Braghieri, A; Branford, D; Briscoe, W J; Brudvik, J; Cherepnya, S; Demissie, B; Downie, E J; Drexler, P; Fil'kov, L V; Fix, A; Glazier, D I; Hamilton, D; Heid, E; Hornidge, D; Howdle, D; Huber, G M; Jaegle, I; Jahn, O; Jude, T C; Käser, A; Kashevarov, V L; Kondratiev, R; Korolija, M; Kruglov, S P; Krusche, B; Kulbardis, A; Lisin, V; Livingston, K; MacGregor, I J D; Maghrbi, Y; Mancell, J; Manley, D M; Marinides, Z; Martinez, M; McGeorge, J C; McNicoll, E; Mekterovic, D; Metag, V; Micanovic, S; Middleton, D G; Mushkarenkov, A; Nefkens, B M K; Nikolaev, A; Novotny, R; Oberle, M; Ostrick, M; Oussena, B; Pedroni, P; Pheron, F; Polonski, A; Prakhov, S N; Robinson, J; Rosner, G; Rostomyan, T; Schumann, S; Sikora, M H; Sober, D; Starostin, A; Supek, I; Thiel, M; Thomas, A; Unverzagt, M; Watts, D P; Werthmüller, D; Witthauer, L

    2014-04-11

    Precise angular distributions have been measured for the first time for the photoproduction of π0 mesons off neutrons bound in the deuteron. The effects from nuclear Fermi motion have been eliminated by a complete kinematic reconstruction of the final state. The influence of final-state-interaction effects has been estimated by a comparison of the reaction cross section for quasifree protons bound in the deuteron to the results for free protons and then applied as a correction to the quasifree neutron data. The experiment was performed at the tagged photon facility of the Mainz Microtron MAMI with the Crystal Ball and TAPS detector setup for incident photon energies between 0.45 and 1.4 GeV. The results are compared to the predictions from reaction models and partial-wave analyses based on data from other isospin channels. The model predictions show large discrepancies among each other and the present data will provide much tighter constraints. This is demonstrated by the results of a new analysis in the framework of the Bonn-Gatchina coupled-channel analysis which included the present data.

  9. Helicity Asymmetry E In Eta (547) Meson Photoproduction From The Proton

    NASA Astrophysics Data System (ADS)

    Morrison, Brian

    The nucleon resonance spectrum consists of many overlapping excitations. Polarization observables are an important tool for understanding and clarifying these spectra. While there is a large data base of differential cross sections for the process, very few data exist for polarization observables. A program of double polarization experiments has been conducted at Jefferson Lab using a tagged polarized photon beam and a frozen spin polarized target (FROST). The results presented here were taken during the first running period of FROST using the CLAS detector at Jefferson Lab with photon energies ranging from 329 MeV to 2.35 GeV. Data are presented for the E polarization observable for eta meson photoproduction on the proton from threshold ( W=1500 MeV) to W=1900 MeV. Comparisons to the partial wave analyses of SAID and Bonn-Gatchina along with the isobar analysis of eta-MAID are made. These results will help distinguish between current theoretical predictions and refine future theories.

  10. Solvent dependence of the photophysical properties of 2-Chlorothioxanthone, the principal photoproduct of chlorprothixene

    PubMed Central

    Piñero Santiago, Luis E.; García, Carmelo; Lhiaubet-Vallet, Virginie; Miranda, Miguel A.; Oyola, Rolando

    2011-01-01

    2-Chlorothioxanthone (CTX) is used as photoinitiator for the reticulation of synthetic resins and for the preparation of pharmaceuticals. It was previously determined that CTX is the primary photoproduct of z-chlorprothixene (CPTX) when irradiated at 313 nm and is formed in an auto-catalyzed reaction through an energy transfer mechanism [Piñero, L. E. et. al. (2009) Photochem. Photobiol. 85, 895–900]. In this work, the photophysical properties of CTX were measured in acetonitrile/water solutions to determine if their magnitude can affect the side-effects of CPTX. The results show that CTX has higher absorption coefficients in the visible region (400–420 nm) and higher triplet quantum yields than its parent compound. Similar to for TX, both properties strongly depend on the solvent polarity/hydroxylicity. The quantum yield of the triplet intermediate is very closed to the value of the phenothiazine triplets. The phenothiazines are the most phototoxic antidepressants. Therefore, given the appropriate microenvironment, the photosensitization side-effects of CPTX can be intensified on the production of CTX. PMID:21294748

  11. Low-energy {omega} ({yields}{pi}{sup 0}{gamma}) meson photoproduction in the nucleus

    SciTech Connect

    Das, Swapan

    2011-06-15

    The {pi}{sup 0{gamma}} invariant mass distribution spectra in the ({gamma},{pi}{sup 0{gamma}}) reaction were measured by the TAPS/ELSA Collaboration to look for the hadron parameters of the {omega} meson in the Nb nucleus. We study the mechanism for this reaction, where we consider that the elementary reaction in the Nb nucleus proceeds as {gamma}N{yields}{omega}N;{omega}{yields}{pi}{sup 0}{gamma}. The {omega}-meson photoproduction amplitude for this reaction is extracted from the measured four-momentum transfer distribution in the {gamma}p{yields}{omega}p reaction. The propagation of the {omega} meson and the distorted wave function for the {pi}{sup 0} meson in the final state are described by the eikonal form. The {omega} and {pi}{sup 0} mesons' nucleus optical potentials, appearing in the {omega} meson propagator and {pi}{sup 0} meson distorted wave function respectively, are estimated using the t{rho} approximation. The effects of pair correlation and color transparency are also studied. The calculated results do not show medium modification for the {omega} meson produced in the nucleus for momentum greater than 200 MeV. It occurs because the {omega} meson predominantly decays outside the nucleus. The dependence of the cross section on the final-state interaction is also investigated. The broadening of the {omega}-meson mass distribution spectra is shown to occur due to the large resolution width associated with the detector used in the experiment.

  12. Helicity asymmetry E measurement for single pi^0 photoproduction with a frozen spin target

    SciTech Connect

    Hideko Iwamoto

    2012-04-01

    The helicity asymmetry for single neutral pion photoproduction was measured using the CLAS detector in Hall B at the Thomas Jefferson National Accelerator Facility. This measurement used longitudinally polarized protons and circularly polarized photons with photon energies between 0.35 GeV to 2.4 GeV. The target was a frozen-spin butanol (C{sub 4}H{sub 9}OH) target, polarized at about 85%. The helicity asymmetry E for the {gamma}p {yields} p{pi}{sup 0} was measured with missing-mass technique at the high statistics of about 12 x 10{sup 6} events. The experimental results are compared to three available theoretical predictions, SAID, MAID, and EBAC. The preliminary results are in good agreement with the model calculations at low E{sub {gamma}} energy bins. However, a significant deviation is observed at high energy bins. Therefore, the new data will help to constrain the parameters of the theoretical models.

  13. Photoproduction of I2, Br2, and Cl2 on n-semiconducting powder

    NASA Technical Reports Server (NTRS)

    Reichman, B.; Byvik, C. E.

    1981-01-01

    The photosynthetic production of Br2 and Cl2 and the photocatalytic production of I2 from aqueous solutions of the respective halide ions in the presence of platinized semiconducting n-TiO2 powder are reported. Reactions were produced in 2-3 M oxygen-saturated aqueous solutions of KI, KBr or NaCl containing Pt-TiO2 powder which were irradiated by a high-pressure mercury lamp at a power of 400 mW/sq cm. Halogens are found to be produced in greater quantities when platinized TiO2 powders are used rather than pure TiO2, and rates of halogen production are observed to increase from Cl2 to Br2 to I2. The presence of the synthetic reactions producing Br2 and Cl2 with a net influx of energy indicates that an effective separation of the photoproduced electron-hole pair occurs in the semiconductor. Quantum efficiencies of the reaction, which increase with decreasing solution pH, are found to be as high as 30%, implying a solar-to-chemical energy conversion efficiency between 0.03% and 3% for the case of chlorine production. It is concluded that the photoproduction of halogens may be of practical value if product halogens are efficiently removed from the reaction cell.

  14. Scalar isovector resonance photoproduction through the final state meson-meson interactions

    NASA Astrophysics Data System (ADS)

    Bibrzycki, Łukasz; Kamiński, Robert

    2016-08-01

    We construct the amplitudes of πη photoproduction taking into account the effects of the πη-KK¯ interchannel coupling. The idea of our model is to describe the scalar isovectors as dynamically produced in the final state while the initial stage of the reaction being described in terms of meson exchanges. Meson loops which arise this way include not only pseudoscalars but also vector mesons. These amplitudes are used to calculate the S-wave cross-sections and mass distributions in the πη effective mass region corresponding to the scalar resonances a0(980) and a0(1450). The values we obtained for a0(980) are comparable with predictions of other models while the cross-section for a0(1450) is about an order of magnitude larger than prediction based on the quark model. We show that the amplitudes with loops containing vector mesons calculated in the on-shell approximation are not suppressed in contrast to amplitudes containing only pseudoscalar loops. We also estimate the cross-sections for the P- and D-waves in the πη channel.

  15. Extraction of Meson Resonances from Three-pions Photo-production Reactions

    SciTech Connect

    S. X. Nakamura, H. Kamano, T.-S. H. Lee, T. Sato

    2012-12-01

    We have investigated the model dependence of meson resonance properties extracted from the Dalitz-plot analysis of the three-pions photoproduction reactions on the nucleon. Within a unitary model developed in Phys. Rev. D 84, 114019 (2011), we generate Dalitz-plot distributions as data to perform an isobar model fit that is similar to most of the previous analyses of three-pion production reactions. It is found that the resonance positions from the two models agree well when both fit the data accurately, except for the resonance poles near branch points. The residues of the resonant amplitudes extracted from the two models and by the usual Breit-Wigner procedure agree well only for the isolated resonances with narrow widths. For overlapping resonances, most of the extracted residues could be drastically different. Our results suggest that even with high precision data, the resonance extraction should be based on models within which the amplitude parametrization is constrained by three-particle unitarity condition.

  16. Nuclear Photoproduction of Pseudoscalar Mesons at Forward Angles up to 6.0 GeV

    SciTech Connect

    Rodrigues, T. E.; Mesa, J.; Garcia, C.; Shtejer, K.; Dale, D.; Cole, P. L.; Nakagawa, I.; Arruda-Neto, J. D. T

    2007-10-26

    The nuclear incoherent {pi}{sup 0} photoproduction cross section from {sup 12}C is evaluated at forward angles in the 4.0 to 6.0 GeV energy range using the multicollisional intranuclear cascade model MCMC. The model incorporates some improvements in comparison with previous versions associated with the momentum distribution (MD) for light nuclei--extracted from the available (e,e{sup '}p) data--as well as the evaluation of the shadowing effects during the photo-nucleus interaction. The final results of the single and double differential cross sections at forward angles are very sensitive to the MD parameterizations due to the Pauli principle, which largely suppresses the cross sections for low momentum transfer. The attenuation of the nuclear cross section due to pion--nucleus final state interactions is approximately 40% (without nuclear shadowing), which is in nice agreement with the predictions from the Glauber model. The single and double {pi}{sup 0} differential cross sections are presented for possible applications for the interpretation of the inelastic background in the PrimEx experiment at the Jefferson Laboratory.

  17. Hyperon Photoproduction from Polarized H and D: towards a complete N* experiment

    SciTech Connect

    Sandorfi, Andrew M.; Hoblit, S.

    2013-09-01

    New complete experiments in pseudoscalar meson photo-production are being pursued at several laboratories. Here the designation of complete refers to measurements of most if not all of the possible reaction observables, of which there are 16 involving spins of the beam, target and recoil baryon. Hyperon production to Λ or Σ{sup +} final states affords attractive opportunities, since their weak decays provide an efficient self-analysis of their polarization. When the beam and target are also polarized, the resulting triple polarization measurements determine the full suite of observables with a single target orientation. This has been a focus at Jefferson Lab in the recently completed g9/FROST and g14/HDice experiments now under analysis. Multipole analyses of γp->K{sup +}Λ have been carried out with a large though incomplete set of recently published polarization data, and the uniqueness of the extracted amplitudes has been studied. Experiments with realistically achievable uncertainties require a significantly greater number of spin asymmetries than the in-principle minimum needed for a mathematical solution of the amplitude.

  18. Polarisation observables for strangeness photoproduction on a frozen spin target with CLAS at Jefferson Lab

    SciTech Connect

    Stuart Fegan

    2012-04-01

    The FROST experiment at Jefferson Lab used the CLAS detector in Hall B with the intention of performing a complete measurement of polarization observables associated with strangeness photoproduction, in combination with data from previous JLab experiments. This was achieved by utilizing the FROST polarized target in conjunction with polarized photon beams, allowing direct measurement of beam-target double polarization observables. By studying strangeness reactions, such as {gamma}p {yields} K{sup +}{Lambda}{sup 0}, it may be possible to find 'missing' baryon resonances, predicted by symmetric quark models but not observed in previous experiments, whose results are consistent with the di-quark model. It is thought these 'missing' resonances remain undiscovered because they have different coupling strengths for different reaction channels, such as the strangeness reactions, whereas the current data is dominated by studies of pN reactions. Observing these resonances therefore has important implications for our knowledge of the excited states of nucleons, and the models predicting the quark interactions within them. The G polarization observable is one of the beam-target double polarization observables, associated with a longitudinally polarized target and a linearly polarized photon beam, and its measurement for the strangeness reaction {gamma}p {yields} K{sup +}{Lambda}{sup 0} is the focus of the work presented.

  19. Study of the Asymmetric Photoproduction of Charmed Mesons using Data from the FOCUS Experiment

    SciTech Connect

    Vazquez-Valencia, Elsa Fabiola; /CINVESTAV, IPN

    2005-05-01

    Using data from the fixed target charm-photoproduction experiment, Fermilab FOCUS/E831, they studied the asymmetric production of the mesons: D{sup 0}, D{sup +} y D{sub s}{sup +}. Even when the asymmetry in the production of charm particles, defined as the ratio between particles and antiparticles, at next leading order in quantum chromodynamics (QCD) is almost zero, in data we observe asymmetries probably associate dto the fragmentation processes. Since these are the less understood phenomena in QCD, the results in the present work could be of great importance to understand them. We found asymmetries in the production of the mesons D{sup +} and D{sup 0} (statistic significant), for the D{sub s}{sup +} we only observed an asymmetry not conclusive. We also report a study of the production asymmetries vs. the kinematic variables: p{sub T}{sup 2} (square transversal momentum of the particles), p{sub L} or p{sub Z} (longitudinal momentum), x{sub F} (Feynman x) and E{sub {gamma}} (photon beam energy).

  20. On the stability of {omega} phase in Ti-6-22-22S and Ti-6-4 alloys

    SciTech Connect

    Zhang, X.D.; Baeslack, W.A. III; Fraser, H.L.; Wiezorek, J.M.K.; Evans, D.J.

    1999-08-20

    Ti-6Al-2Mo-2Cr-2Sn-2Zr-0.2Si (Ti-6-22-22S) alloy, which was developed initially twenty years ago, has been recently reconsidered as a new moderate temperature material for aircraft structural applications. Ti-6-22-22S is stronger than the conventional Ti-6Al-4V (Ti-6-4) alloy and has better damage tolerance. As a part of the comprehensive program on the microstructural characterization of Ti-6-22-22S, the effect of heat treatment and the addition of small amounts of Si on the microstructural development and mechanical properties have been thoroughly investigated. These heat treatment conditions include variations in the cooling rates from the {beta} solution treatment and ({alpha}+{beta}) solution treatment, the temperatures of ({alpha}+{beta}) solution treatment and aging temperatures. Furthermore the microstructural stability of this alloy, particularly with respect to the precipitation of secondary intermetallic compounds and their effect on the mechanical properties, has been investigated. In previous studies, two types of precipitates, namely silicides and {alpha}{sub 2} phase, have been observed. The stability of these precipitates during long time aging, and partitioning of alloying elements as function of heat treatment as well as their effects on mechanical properties have also been reported. In the present study, observations of the {omega} phase and its stability will be reported for Ti-6-22-22S and the results will be compared with those reported for Ti-6-4 alloy.

  1. Effect of Electron Beam Freeform Fabrication (EBF3) Processing Parameters on Composition of Ti-6-4

    NASA Technical Reports Server (NTRS)

    Lach, Cynthia L.; Taminger, Karen; Schuszler, A. Bud, II; Sankaran, Sankara; Ehlers, Helen; Nasserrafi, Rahbar; Woods, Bryan

    2007-01-01

    The Electron Beam Freeform Fabrication (EBF3) process developed at NASA Langley Research Center was evaluated using a design of experiments approach to determine the effect of processing parameters on the composition and geometry of Ti-6-4 deposits. The effects of three processing parameters: beam power, translation speed, and wire feed rate, were investigated by varying one while keeping the remaining parameters constant. A three-factorial, three-level, fully balanced mutually orthogonal array (L27) design of experiments approach was used to examine the effects of low, medium, and high settings for the processing parameters on the chemistry, geometry, and quality of the resulting deposits. Single bead high deposits were fabricated and evaluated for 27 experimental conditions. Loss of aluminum in Ti-6-4 was observed in EBF3 processing due to selective vaporization of the aluminum from the sustained molten pool in the vacuum environment; therefore, the chemistries of the deposits were measured and compared with the composition of the initial wire and base plate to determine if the loss of aluminum could be minimized through careful selection of processing parameters. The influence of processing parameters and coupling between these parameters on bulk composition, measured by Direct Current Plasma (DCP), local microchemistries determined by Wavelength Dispersive Spectrometry (WDS), and deposit geometry will also be discussed.

  2. Parameterization of Highly Charged Metal Ions Using the 12-6-4 LJ-Type Nonbonded Model in Explicit Water

    PubMed Central

    2015-01-01

    Highly charged metal ions act as catalytic centers and structural elements in a broad range of chemical complexes. The nonbonded model for metal ions is extensively used in molecular simulations due to its simple form, computational speed, and transferability. We have proposed and parametrized a 12-6-4 LJ (Lennard-Jones)-type nonbonded model for divalent metal ions in previous work, which showed a marked improvement over the 12-6 LJ nonbonded model. In the present study, by treating the experimental hydration free energies and ion–oxygen distances of the first solvation shell as targets for our parametrization, we evaluated 12-6 LJ parameters for 18 M(III) and 6 M(IV) metal ions for three widely used water models (TIP3P, SPC/E, and TIP4PEW). As expected, the interaction energy underestimation of the 12-6 LJ nonbonded model increases dramatically for the highly charged metal ions. We then parametrized the 12-6-4 LJ-type nonbonded model for these metal ions with the three water models. The final parameters reproduced the target values with good accuracy, which is consistent with our previous experience using this potential. Finally, tests were performed on a protein system, and the obtained results validate the transferability of these nonbonded model parameters. PMID:25145273

  3. A Rare and Exclusive Endoperoxide Photoproduct Derived from a Thiacalix[4]arene Crown-Shaped Derivative Bearing a 9,10-Substituted Anthracene Moiety.

    PubMed

    Zhao, Jiang-Lin; Wu, Chong; Tomiyasu, Hirotsugu; Zeng, Xi; Elsegood, Mark R J; Redshaw, Carl; Yamato, Takehiko

    2016-05-20

    A rare and exclusive endoperoxide photoproduct was quantitatively obtained from a thiacalix[4]arene crown-shaped derivative upon irradiation at λ=365 nm; the structure was unambiguously confirmed by (1) H/(13) C NMR spectroscopy and X-ray crystallography. The prerequisites for the formation of the endoperoxide photoproduct have also been discussed. Furthermore, the photochemical reaction rate could be greatly enhanced in the presence of the thiacalix[4]arene platform because it served as a host to capture oxygen.

  4. Measurements of C_x and C_z for K^+Lambda and K^+Sigma^o Photoproduction

    SciTech Connect

    Robert Bradford; Reinhard Schumacher

    2005-10-10

    The CLAS collaboration has recently completed first measurements of the double polarization observables C{sub x} and C{sub z} for the reactions K{sup +} Lambda and K{sup +} Sigma{sup o} photoproduction. C{sub x} and C{sub z} are the beam-recoil polarization asymmetries measuring the polarization transfer from incoming circularly polarized photons to the outgoing hyperons along two directions in the reaction's production plane. The Lambda is found to be nearly maximally polarized along the direction of the incident photon's polarization for forward-going kaons. Polarization transfer to the Sigma{sup o} is different from the Lambda case.

  5. Light Vector Meson Photoproduction off of H at Jefferson Lab and rho-omega Interference in the Leptonic Decay Channel

    SciTech Connect

    Chaden Djalali

    2011-12-01

    Recent studies of light vector meson production in heavy nuclear targets has generated interest in {rho}-{omega} interference in the leptonic e{sup +}e{sup -} decay channel. An experimental study of the elementary process provides valuable input for theoretical models and calculations. In experiment E04-005 (g12), high statistics photoproduction data has been taken in Jefferson Lab's Hall B with the Cebaf Large Acceptance Spectrometer (CLAS). The invariant mass spectrum is fitted with two interfering relativistic Breit-Wigner functions to determine the interference phase. Preliminary analysis indicate a measurable {rho}-{omega} interference.

  6. Double Polarization Observable E in η, π0, and 2π0 Photoproduction off Protons and Neutrons

    NASA Astrophysics Data System (ADS)

    Dieterle, Manuel; Witthauer, Lilian

    Helicity dependent cross sections σ1/2, σ3/2, and the polarization observable E for π0, π0π0, and η photoproduction from quasi-free protons and neutrons in the second and third nucleon resonance region were measured for the first time at the electron accelerator facility MAMI. Different methods for the extraction of the observables were used to determine the systematics of the measurements. The results will provide stringent constraints on the resonances that contribute to the excitation spectrum of the nucleon and will clearly help to improve its theoretical understanding.

  7. Synthesis, spectral, thermal, optical and theoretical studies of (2E,6E)-2-benzylidene-6-(4-methoxybenzylidene)cyclohexanone.

    PubMed

    Meenatchi, V; Muthu, K; Rajasekar, M; Meenakshisundaram, Sp

    2014-01-01

    Single crystals of (2E,6E)-2-benzylidine-6-(4-methoxybenzylidine)cyclohexanone are grown by slow evaporation of ethanolic solution at room temperature. The characteristic functional groups present in the molecule are confirmed by Fourier transform infrared and Fourier transform Raman analyses. The scanning electron microscopy study reveals the surface morphology of the material. Thermogravimetric/differential thermal analysis study reveals the purity of the material and the crystal is transparent in the visible region having a lower optical cut-off at ∼487nm. The second harmonic generation efficiency of as-grown material is estimated by Kurtz and Perry technique. Optimized geometry has been derived using Hartree-Fock calculations performed at the level 6-31G (d,p) and the first-order molecular hyperpolarizability (β) is estimated. The specimen is further characterized by nuclear magnetic resonance spectroscopy.

  8. Spatiotemporal investigation of seismicity and Coulomb stress variations prior to the 2010 ML 6.4 Jiashian, Taiwan earthquake

    NASA Astrophysics Data System (ADS)

    Wen, Yi-Ying; Chen, Chien-Chih; Wu, Yi-Hsuan; Chan, Chung-Han; Wang, Yu-Ju; Yeh, Yu-Lien

    2016-08-01

    The 2010 ML 6.4 Jiashian earthquake struck southern Taiwan and caused some damage due to the strong ground shaking. Since the epicenter is located in a relatively low background seismicity area, we want to investigate seismicity rate changes associated with the Jiashian main shock by applying the region-time-length and pattern informatics algorithms. Both temporal and spatial results exhibit signatures of abnormal seismicity change related to the seismic activation and quiescence prior to the 2010 Jiashian main shock. In addition, patch of abnormal seismicity changes coincides with the region with Coulomb stress change during the same period. Our study demonstrates that the epicenter area of the 2010 Jiashian earthquake experienced a long period of seismicity rate changes and stress accumulation and thus suggests that both the variations in Coulomb stress and seismicity rate play fundamental roles in the nucleation process of impending earthquakes.

  9. Low cost upgrade of 6.4 GHz ECRIS and recent results with 14 GHz ECRIS at JYFL

    NASA Astrophysics Data System (ADS)

    Koivisto, H.; Heikkinen, P.; Liukkonen, E.; ńrje, J.; Vondrasek, R.

    2002-02-01

    The old JYFL 6.4 GHz electron cyclotron resonance ion source (ECRIS) at the University of Jyväskylä, Department of Physics (JYFL), is in the process of being upgraded. The calculations have shown that the magnetic field configuration of the SC-ECRIS at the NSCL/MSU can be closely achieved using present coils, power supplies, plasma chamber, and permanent magnets. The hexapole magnetic field will be upgraded later using an iron cylinder around the plasma chamber. Calculations have shown that the improvement of around 30% can be obtained to the strength of the hexapole field. A new AECR-U-type ion source was completed in spring 2000 for the program of the nuclear physics in the laboratory. The MIVOC method and the internal oven for the production of several metal ion beams with the new source have been tested.

  10. Multiple fault slip triggered above the 2016 Mw 6.4 MeiNong earthquake in Taiwan

    NASA Astrophysics Data System (ADS)

    Huang, Mong-Han; Tung, Hsin; Fielding, Eric J.; Huang, Hsin-Hua; Liang, Cunren; Huang, Chung; Hu, Jyr-Ching

    2016-07-01

    Rapid shortening in convergent mountain belts is often accommodated by slip on faults at multiple levels in upper crust, but no geodetic observation of slip at multiple levels within hours of a moderate earthquake has been shown before. Here we show clear evidence of fault slip within a shallower thrust at 5-10 km depth in SW Taiwan triggered by the 2016 Mw 6.4 MeiNong earthquake at 15-20 km depth. We constrain the primary coseismic fault slip with kinematic modeling of seismic and geodetic measurements and constrain the triggered slip and fault geometry using synthetic aperture radar interferometry. The shallower thrust coincides with a proposed duplex located in a region of high fluid pressure and high interseismic uplift rate, and may be sensitive to stress perturbations. Our results imply that under tectonic conditions such as high-background stress level and high fluid pressure, a moderate lower crustal earthquake can trigger faults at shallower depth.

  11. Hydrogen film cooling with incident and swept-shock interactions in a Mach 6.4 nitrogen free stream

    NASA Technical Reports Server (NTRS)

    Olsen, George C.; Nowak, Robert J.

    1995-01-01

    The effectiveness of slot film cooling of a flat plate in a Mach 6.4 flow with and without incident and swept oblique shock interactions was experimentally investigated. Hydrogen was the primary coolant gas, although some tests were conducted using helium as the coolant. Tests were conducted in the Calspan 48-Inch Shock Tunnel with a nitrogen flow field to preclude combustion of the hydrogen coolant gas. A two-dimensional highly instrumented model developed in a previous test series was used. Parameters investigated included coolant mass flow rate, coolant gas, local free-stream Reynolds number, incident oblique shock strength, and a swept oblique shock. Both gases were highly effective coolants in undisturbed flow; however, both incident and swept shocks degraded that effectiveness.

  12. Anthelmintic profile of methyl 5(6)-(4-methylpiperidin-1-yl) carbonylbenzimidazole-2-carbamate in experimental helminthiases.

    PubMed

    Gupta, S; Khan, A M; Jain, M K; Katiyar, J C; Naim, S S; Singh, S K; Sharma, S

    1990-05-01

    Biological evaluation of methyl 5(6)-(4-methylpiperidin-1-yl) carbonylbenzimidazole-2-carbamate against Ancylostoma ceylanicum, Nippostrongylus brasiliensis, Syphacia obvelata, Hymenolepis nana, H. diminuta and Cysticercus fasciolaris in experimental animals is reported. The compound (mg/kg) causes 100% elimination of A. ceylanicum (25 x 1), N. brasiliensis (100 x 1), S. obvelata (50 x 1), H. nana (250 x 3) and C. fasciolaris (50 x 10). It was also effective against the developing larvae (L3, L4 and L5) of A. ceylanicum at a single oral dose of 100 mg/kg. Another study indicated that the compound elicits 100% response within 32 hr of drug administration. The drug is well tolerated and LD50 is greater than 4500 mg/kg.

  13. Sensitizing effect of Z,Z-bilirubin IXα and its photoproducts on enzymes in model solutions

    NASA Astrophysics Data System (ADS)

    Plavskii, V. Yu.; Mostovnikov, V. A.; Tret'yakova, A. I.; Mostovnikova, G. R.

    2008-05-01

    In model systems, we have studied side effects which may be induced by light during phototherapy of hyperbilirubinemia (jaundice) in newborn infants, with the aim of reducing the Z,Z-bilirubin IXα (Z,Z-BR IXα) level. We have shown that the sensitizing effect of Z,Z-BR IXα, localized at strong binding sites of the human serum albumin (HSA) macromolecule, is primarily directed at the amino acid residues of the carrier protein and does not involve the molecules of the enzyme (lactate dehydrogenase (LDH)) present in the buffer solution. The detected photodynamic damage to LDH is due to sensitization by bilirubin photoisomers, characterized by lower HSA association constants and located (in contrast to native Z,Z-BR IXα) on the surface of the HSA protein globule. Based on study of the spectral characteristics of the photoproducts of Z,Z-BR IXα and comparison of their accumulation kinetics in solution and the enzyme photo-inactivation kinetics, we concluded that the determining role in sensitized damage to LDH is played by lumirubin. The photosensitization effect depends on the wavelength of the radiation used for photoconversion of bilirubin. When (at the beginning of exposure) we make sure that identical numbers of photons are absorbed by the pigment in the different spectral ranges, the side effect is minimal for radiation corresponding to the long-wavelength edge of the bilirubin absorption band. We have shown that for a bilirubin/HSA concentration ratio >2 (when some of the pigment molecules are sorbed on the surface of the protein globule), the bilirubin can act as a photosensitizing agent for the enzyme present in solution. We discuss methods for reducing unfavorable side effects of light on the body of newborn infants during phototherapy of hyperbilirubinemia.

  14. Near-threshold incoherent ϕ photoproduction on the deuteron: Searching for traces of a resonance

    NASA Astrophysics Data System (ADS)

    Kiswandhi, Alvin; Yang, Shin Nan; Dong, Yu Bing

    2016-07-01

    We study the near-threshold incoherent ϕ photoproduction on the deuteron based on a model of γ N →ϕ N , consisting of Pomeron, (π ,η ) exchanges, and a JP=3 /2- resonance, which describes the low-energy γ p →ϕ p LEPS data well, including the peak in the forward differential cross section. The calculation is done up to double rescatterings, with the spin dependence of the elementary γ N →ϕ N amplitude retained throughout the calculation. The Fermi motion and final-state interactions (FSIs) are all properly treated as prescribed by realistic nucleon-nucleon interaction. The couplings of the resonance to γ n and ϕ n channels are estimated with the help of a constituent quark model. The main features of the LEPS and CLAS data are described reasonably well except for some quantitative discrepancies at very low energies and low-momentum-transfer regions. It is found that contributions of Fermi motion, p n FSI, and resonance are all indispensable in bridging the differences between the single-scattering results and the data. The off-shell rescattering is found to be important because it cancels out a large portion of the on-shell contribution. The discrepancies at low-momentum-transfer regions might be related to the binning size of the data. No peak is found to be associated with the weak resonance because it gets smeared out by the Fermi motion and FSI with the deuterium target. The problem at very-low-energy regions hints at the possible contributions from other mechanisms and should be investigated in depth with the use of recent high-statistics γ p →ϕ p data from CLAS.

  15. Increased Nitrogenase-Dependent H(2) Photoproduction by hup Mutants of Rhodospirillum rubrum.

    PubMed

    Kern, M; Klipp, W; Klemme, J H

    1994-06-01

    Transposon Tn5 mutagenesis was used to isolate mutants of Rhodospirillum rubrum which lack uptake hydrogenase (Hup) activity. Three Tn5 insertions mapped at different positions within the same 13-kb EcoRI fragment (fragment E1). Hybridization experiments revealed homology to the structural hydrogenase genes hupSLM from Rhodobacter capsulatus and hupSL from Bradyrhizobium japonicum in a 3.8-kb EcoRI-ClaI subfragment of fragment E1. It is suggested that this region contains at least some of the structural genes encoding the nickel-dependent uptake hydrogenase of R. rubrum. At a distance of about 4.5 kb from the fragment homologous to hupSLM, a region with homology to a DNA fragment carrying hypDE and hoxXA from B. japonicum was identified. Stable insertion and deletion mutations were generated in vitro and introduced into R. rubrum by homogenotization. In comparison with the wild type, the resulting hup mutants showed increased nitrogenase-dependent H(2) photoproduction. However, a mutation in a structural hup gene did not result in maximum H(2) production rates, indicating that the capacity to recycle H(2) was not completely lost. Highest H(2) production rates were obtained with a mutant carrying an insertion in a nonstructural hup-specific sequence and with a deletion mutant affected in both structural and nonstructural hup genes. Thus, besides the known Hup activity, a second, previously unknown Hup activity seems to be involved in H(2) recycling. A single regulatory or accessory gene might be responsible for both enzymes. In contrast to the nickel-dependent uptake hydrogenase, the second Hup activity seems to be resistant to the metal chelator EDTA. PMID:16349271

  16. The Subfamily-Specific Interaction between Kv2.1 and Kv6.4 Subunits Is Determined by Interactions between the N- and C-termini

    PubMed Central

    Bocksteins, Elke; Mayeur, Evy; Van Tilborg, Abbi; Regnier, Glenn; Timmermans, Jean-Pierre; Snyders, Dirk J.

    2014-01-01

    The “silent” voltage-gated potassium (KvS) channel subunit Kv6.4 does not form electrically functional homotetramers at the plasma membrane but assembles with Kv2.1 subunits, generating functional Kv2.1/Kv6.4 heterotetramers. The N-terminal T1 domain determines the subfamily-specific assembly of Kv1-4 subunits by preventing interactions between subunits that belong to different subfamilies. For Kv6.4, yeast-two-hybrid experiments showed an interaction of the Kv6.4 N-terminus with the Kv2.1 N-terminus, but unexpectedly also with the Kv3.1 N-terminus. We confirmed this interaction by Fluorescence Resonance Energy Transfer (FRET) and co-immunoprecipitation (co-IP) using N-terminal Kv3.1 and Kv6.4 fragments. However, full-length Kv3.1 and Kv6.4 subunits do not form heterotetramers at the plasma membrane. Therefore, additional interactions between the Kv6.4 and Kv2.1 subunits should be important in the Kv2.1/Kv6.4 subfamily-specificity. Using FRET and co-IP approaches with N- and C-terminal fragments we observed that the Kv6.4 C-terminus physically interacts with the Kv2.1 N-terminus but not with the Kv3.1 N-terminus. The N-terminal amino acid sequence CDD which is conserved between Kv2 and KvS subunits appeared to be a key determinant since charge reversals with arginine substitutions abolished the interaction between the N-terminus of Kv2.1 and the C-terminus of both Kv2.1 and Kv6.4. In addition, the Kv6.4(CKv3.1) chimera in which the C-terminus of Kv6.4 was replaced by the corresponding domain of Kv3.1, disrupted the assembly with Kv2.1. These results indicate that the subfamily-specific Kv2.1/Kv6.4 heterotetramerization is determined by interactions between Kv2.1 and Kv6.4 that involve both the N- and C-termini in which the conserved N-terminal CDD sequence plays a key role. PMID:24901643

  17. Nucleotide excision repair is reduced in oral epithelial tissues compared with skin.

    PubMed

    Mitchell, David; Paniker, Lakshmi; Godar, Dianne

    2012-01-01

    Ultraviolet radiation (UVR) exposure to internal tissues for diagnostic, therapeutic and cosmetic procedures has increased dramatically over the past decade. The greatest increase in UVR exposure of internal tissues occurs in the cosmetic industry where it is combined with oxidizing agents for teeth whitening, often in conjunction with indoor tanning. To address potential carcinogenic risks of these procedures, we analyzed the formation and repair of the DNA photoproducts associated with the signature mutations of UVR. Radioimmunoassay was used to quantify the induction and repair of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts in DNA purified from three reconstructed tissues, EpiDerm(TM) , EpiGingival(TM) and EpiOral(TM) . We observed comparable levels of DNA damage in all tissues immediately after UVR exposure. In contrast, repair was significantly reduced in both oral tissues compared with EpiDerm(TM) . Our data suggest that UVR exposure of oral tissues can result in accumulation of DNA damage and increase the risk for carcinoma and melanoma of the mouth. Because NER is a broad-spectrum defense against DNA damage caused by a variety of agents in addition to UVR, our data suggest that the relatively low NER efficiency observed in oral tissues may have wide-ranging consequences in this highly exposed environment.

  18. Nucleotide excision repair is reduced in oral epithelial tissues compared to skin‡

    PubMed Central

    Mitchell, David; Paniker, Lakshmi; Godar, Dianne

    2012-01-01

    Ultraviolet radiation (UVR) exposure to internal tissues for diagnostic, therapeutic and cosmetic procedures has increased dramatically over the past decade. The greatest increase in UVR exposure of internal tissues occurs in the cosmetic industry where it is combined with oxidizing agents for teeth whitening, often in conjunction with indoor tanning. To address potential carcinogenic risks of these procedures, we analyzed the formation and repair of the DNA photoproducts associated with the signature mutations of UVR. Radioimmunoassay was used to quantify the induction and repair of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts in DNA purified from three reconstructed tissues, EpiDerm™, EpiGingival™, and EpiOral™ (MatTek Corp.). We observed comparable levels of DNA damage in all tissues immediately after UVR exposure. In contrast, repair was significantly reduced in both oral tissues compared to EpiDerm™. Our data suggest that UVR exposure of oral tissues can result in accumulation of DNA damage and increase the risk for carcinoma and melanoma of the mouth. Because NER is a broad-spectrum defense against DNA damage caused by a variety of agents in addition to UVR, our data suggest that the relatively low NER efficiency observed in oral tissues may have wide-ranging consequences in this highly exposed environment. PMID:22519509

  19. Insight into the Narrow Structure in η Photoproduction on the Neutron from Helicity-Dependent Cross Sections

    NASA Astrophysics Data System (ADS)

    Witthauer, L.; Dieterle, M.; Abt, S.; Achenbach, P.; Afzal, F.; Ahmed, Z.; Annand, J. R. M.; Arends, H. J.; Bashkanov, M.; Beck, R.; Biroth, M.; Borisov, N. S.; Braghieri, A.; Briscoe, W. J.; Cividini, F.; Costanza, S.; Collicott, C.; Denig, A.; Downie, E. J.; Drexler, P.; Ferretti-Bondy, M. I.; Gardner, S.; Garni, S.; Glazier, D. I.; Glowa, D.; Gradl, W.; Günther, M.; Gurevich, G. M.; Hamilton, D.; Hornidge, D.; Huber, G. M.; Käser, A.; Kashevarov, V. L.; Kay, S.; Keshelashvili, I.; Kondratiev, R.; Korolija, M.; Krusche, B.; Lazarev, A. B.; Linturi, J. M.; Lisin, V.; Livingston, K.; Lutterer, S.; MacGregor, I. J. D.; Mancell, J.; Manley, D. M.; Martel, P. P.; Metag, V.; Meyer, W.; Miskimen, R.; Mornacchi, E.; Mushkarenkov, A.; Neganov, A. B.; Neiser, A.; Oberle, M.; Ostrick, M.; Otte, P. B.; Paudyal, D.; Pedroni, P.; Polonski, A.; Prakhov, S. N.; Rajabi, A.; Reicherz, G.; Ron, G.; Rostomyan, T.; Sarty, A.; Sfienti, C.; Sikora, M. H.; Sokhoyan, V.; Spieker, K.; Steffen, O.; Strakovski, I. I.; Strub, Th.; Supek, I.; Thiel, A.; Thiel, M.; Thomas, A.; Unverzagt, M.; Usov, Yu. A.; Wagner, S.; Walford, N. K.; Watts, D. P.; Werthmüller, D.; Wettig, J.; Wolfes, M.; Zana, L.; A2 Collaboration at MAMI

    2016-09-01

    The double polarization observable E and the helicity dependent cross sections σ1 /2 and σ3 /2 were measured for η photoproduction from quasifree protons and neutrons. The circularly polarized tagged photon beam of the A2 experiment at the Mainz MAMI accelerator was used in combination with a longitudinally polarized deuterated butanol target. The almost 4 π detector setup of the Crystal Ball and TAPS is ideally suited to detect the recoil nucleons and the decay photons from η →2 γ and η →3 π0. The results show that the narrow structure previously observed in η photoproduction from the neutron is only apparent in σ1 /2 and hence, most likely related to a spin-1 /2 amplitude. Nucleon resonances that contribute to this partial wave in η production are only N 1 /2- (S11) and N 1 /2+ (P11). Furthermore, the extracted Legendre coefficients of the angular distributions for σ1 /2 are in good agreement with recent reaction model predictions assuming a narrow resonance in the P11 wave as the origin of this structure.

  20. Hydrogen peroxide photoproduction by immobilized cells of the blue-green alga Anabaena variabilis: A way to solar energy conversion

    SciTech Connect

    Morales, I.; La Rosa, F.F. de )

    1992-07-01

    A photosystem for hydrogen peroxide photoproduction formed by immobilized cells of the blue-green alga, Anabaena variabilis and the redox mediator methyl viologen is described. Hydrogen peroxide is produced in a redox catalyst cycle in which methyl viologen is reduced by electrons from water obtained by the photosynthetic apparatus of the algae using solar energy, and reoxidized by the introduction of oxygen into the solution. Hydrogen peroxide is produced during methyl viologen re-oxidation in two steps by means of the formation of superoxide. Experimental conditions for maximum photoproduction (catalyst charge, chlorophyll, and agar final concentration for cell immobilization) have been investigated using a continuous photosystem with immobilized A. variabilis as photocatalyst. Under the determined optimum conditions, the photosystem with immobilized A. variabilis is photocatalyst. Under the determined optimum conditions, the photosystem produces hydrogen peroxide at a rate of 100 {mu}moles/mg Chl{center dot}h, maintaining the production for several hours, and with an energy conversion efficiency of about 2%. Taking into account the use of hydrogen peroxide as fuel, this photosystem can be a useful tool in the storage of solar energy.

  1. Control of Hydrogen Photoproduction by the Proton Gradient Generated by Cyclic Electron Flow in Chlamydomonas reinhardtii[W

    PubMed Central

    Tolleter, Dimitri; Ghysels, Bart; Alric, Jean; Petroutsos, Dimitris; Tolstygina, Irina; Krawietz, Danuta; Happe, Thomas; Auroy, Pascaline; Adriano, Jean-Marc; Beyly, Audrey; Cuiné, Stéphan; Plet, Julie; Reiter, Ilja M.; Genty, Bernard; Cournac, Laurent; Hippler, Michael; Peltier, Gilles

    2011-01-01

    Hydrogen photoproduction by eukaryotic microalgae results from a connection between the photosynthetic electron transport chain and a plastidial hydrogenase. Algal H2 production is a transitory phenomenon under most natural conditions, often viewed as a safety valve protecting the photosynthetic electron transport chain from overreduction. From the colony screening of an insertion mutant library of the unicellular green alga Chlamydomonas reinhardtii based on the analysis of dark-light chlorophyll fluorescence transients, we isolated a mutant impaired in cyclic electron flow around photosystem I (CEF) due to a defect in the Proton Gradient Regulation Like1 (PGRL1) protein. Under aerobiosis, nonphotochemical quenching of fluorescence (NPQ) is strongly decreased in pgrl1. Under anaerobiosis, H2 photoproduction is strongly enhanced in the pgrl1 mutant, both during short-term and long-term measurements (in conditions of sulfur deprivation). Based on the light dependence of NPQ and hydrogen production, as well as on the enhanced hydrogen production observed in the wild-type strain in the presence of the uncoupling agent carbonyl cyanide p-trifluoromethoxyphenylhydrazone, we conclude that the proton gradient generated by CEF provokes a strong inhibition of electron supply to the hydrogenase in the wild-type strain, which is released in the pgrl1 mutant. Regulation of the trans-thylakoidal proton gradient by monitoring pgrl1 expression opens new perspectives toward reprogramming the cellular metabolism of microalgae for enhanced H2 production. PMID:21764992

  2. Probing the gluon density of the proton in the exclusive photoproduction of vector mesons at the LHC: a phenomenological analysis

    NASA Astrophysics Data System (ADS)

    Gonçalves, V. P.; Martins, L. A. S.; Sauter, W. K.

    2016-02-01

    The current uncertainty on the gluon density extracted from the global parton analysis is large in the kinematical range of small values of the Bjorken- x variable and low values of the hard scale Q^2. An alternative to reduces this uncertainty is the analysis of the exclusive vector meson photoproduction in photon-hadron and hadron-hadron collisions. This process offers a unique opportunity to constrain the gluon density of the proton, since its cross section is proportional to the gluon density squared. In this paper we consider current parametrisations for the gluon distribution and estimate the exclusive vector meson photoproduction cross section at HERA and LHC using the leading logarithmic formalism. We perform a fit of the normalisation of the γ h cross section and the value of the hard scale for the process and demonstrate that the current LHCb experimental data are better described by models that assume a slow increasing of the gluon distribution at small x and low Q^2.

  3. Autotrophic hydrogen photoproduction by operation of carbon-concentrating mechanism in Chlamydomonas reinhardtii under sulfur deprivation condition.

    PubMed

    Hong, Min Eui; Shin, Ye Sol; Kim, Byung Woo; Sim, Sang Jun

    2016-03-10

    Under autotrophic conditions, starch plays an important role in establishing anoxic conditions during PSII-dependent hydrogen (H2) photoproduction in microalgae. This is because starch is the sole organic substrate during respiratory consumption of internal oxygen (O2) from PSII-dependent direct pathway. Herein, we propose a novel approach to further facilitate the internal starch synthesis of Chlamydomonas reinhardtii through the operation of carbon-concentrating mechanism (CCM) along with a two-stage process based on sulfur (S) deprivation, thereby resulting in enhanced anaerobic capacity during PSII-dependent H2 photoproduction. When CCM-induced cells were exposed to high levels of carbon dioxide (CO2) (5%, v/v) with S deprivation, internal levels of starch were significantly elevated by retaining a functional CCM with the boosted photosynthetic activity during 24h of O2 evolution phase (I) of S deprivation. Consequently, during H2 production phase of S deprivation at irradiance of 50μEm(-2)s(-1), the concentrations of starch and H2 in CCM-induced cells were remarkably enhanced by 65.0% and 218.9% compared to that of CCM-uninduced cells, respectively. The treatment of low-CO2-driven CCM induction prior to S deprivation is a cost-effective and energy-efficient strategy that significantly improves the solar-driven H2 production by microalgae; this is particularly realizable in an industrial scale. PMID:26812657

  4. The effect of sulfur re-addition on H(2) photoproduction by sulfur-deprived green algae.

    PubMed

    Kosourov, Sergey; Makarova, Valeriya; Fedorov, Alexander S; Tsygankov, Anatoly; Seibert, Michael; Ghirardi, Maria L

    2005-09-01

    Sulfur deprivation of algal cultures selectively and partially inactivates photosystem II (PSII)-catalyzed O(2) evolution, induces anaerobiosis and hydrogenase expression, and results in sustained H(2) photoproduction for several days. We show that re-addition of limiting amounts of sulfate (1-10 microM final concentration) to the cultures during the H(2)-production phase temporarily reactivates PSII photochemical and O(2)-evolution activity and re-establishes higher rates of electron transport through the photosynthetic electron transport chain. The reactivation of PSII occurs by de novo D1 protein synthesis, but does not result in the re-establishment of aerobic conditions in the reactor, detectable by dissolved-O(2) sensors. However, concomitant H(2) photoproduction is inhibited, possibly due to excessive intra-cellular levels of photosynthetically-evolved O(2). The partial recovery of electron transport rates correlates with the re-oxidation of the plastoquinone (PQ) pool, as observed by pulse-amplitude modulated (PAM) and fluorescence-induction measurements. These results show that the presence of a more oxidized PQ pool releases some of the down-regulation of electron transport caused by the anaerobic conditions. PMID:16170632

  5. p{sup 0} photoproduction in ultraperipheral relativistic heavy ion collisions at {radical}{ovr s}{sub NN} = 200 GeV.

    SciTech Connect

    Abelev, B. I.; Aggarwal, M. M.; Ahammed, Z.; Anderson, B. D.; Arkhipkin, D.; Krueger, K.; Spinka, H. M.; Underwood, D. G.; STAR Collaboration; High Energy Physics; Univ. of Illinois; Panjab Univ.; Variable Energy Cyclotron Centre; Kent State Univ.; Particle Physic Lab.

    2008-01-01

    Photoproduction reactions occur when the electromagnetic field of a relativistic heavy ion interacts with another heavy ion. The STAR Collaboration presents a measurement of {rho}{sup 0} and direct {pi}{sup +}{pi}{sup -} photoproduction in ultraperipheral relativistic heavy ion collisions at {radical}s{sub NN} = 200 GeV. We observe both exclusive photoproduction and photoproduction accompanied by mutual Coulomb excitation. We find a coherent cross section of {sigma} (AuAu {yields} Au{sup +}Au{sup +} {rho}{sup 0}) = 530 {+-} 19(stat.) {+-} 57(syst.) mb, in accord with theoretical calculations based on a Glauber approach, but considerably below the predictions of a color dipole model. The {rho}{sup p} transverse momentum spectrum (p{sub T}{sup 2}) is fit by a double exponential curve including both coherent and incoherent coupling to the target nucleus; we find {sigma}{sub inc}/{sigma}{sub coh} = 0.29 {+-} 0.03(stat.) {+-} 0.08(syst.). The ratio of direct {pi}{sup +}{pi}{sup -} to {rho}{sup 0} production is comparable to that observed in {gamma}{sub p} collisions at HERA and appears to be independent of photon energy. Finally, the measured {rho}{sup 0} spin helicity matrix elements agree within errors with the expected s-channel helicity conservation.

  6. Identification of unwanted photoproducts of cosmetic preservatives in personal care products under ultraviolet-light using solid-phase microextraction and micro-matrix solid-phase dispersion.

    PubMed

    Alvarez-Rivera, Gerardo; Llompart, Maria; Garcia-Jares, Carmen; Lores, Marta

    2015-04-17

    The photochemical transformation of widely used cosmetic preservatives including benzoates, parabens, BHA, BHT and triclosan has been investigated in this work applying an innovative double-approach strategy: identification of transformation products in aqueous photodegradation experiments (UV-light, 254nm), followed by targeted screening analysis of such photoproducts in UV-irradiated cosmetic samples. Solid-phase microextraction (SPME) was applied, using different fiber coatings, in order to widen the range of detectable photoproducts in water, whereas UV-irradiated personal care products (PCPs) containing the target preservatives were extracted by micro-matrix solid-phase dispersion (micro-MSPD). Both SPME and micro-MSPD-based methodologies were successfully optimized and validated. Degradation kinetics of parent species, and photoformation of their transformation by-products were monitored by gas chromatography coupled to mass spectrometry (GC-MS). Thirty nine photoproducts were detected in aqueous photodegradation experiments, being tentatively identified based on their mass spectra. Transformation pathways between structurally related by-products, consistent with their kinetic behavior were postulated. The photoformation of unexpected photoproducts such as 2- and 4-hydroxybenzophenones, and 2,8-dichlorodibenzo-p-dioxin in PCPs are reported in this work for the first time.

  7. The 29 July 2014 (Mw 6.4) Southern Veracruz, Mexico Earthquake: Scenary Previous to Its Occurrence.

    NASA Astrophysics Data System (ADS)

    Yamamoto, J.

    2014-12-01

    On 29 July 2014 (10:46 UTC) a magnitude 6.4 (Mw) earthquake occurred at the southern Veracruz, Mexico region. The epicenter was preliminary located at 17.70° N and 95.63° W. It was a normal fault event with the slip on a fault that trend NNW and a focus approximately 117 km below the surface of the Gulf of Mexico costal plane. The earthquake was widely felt through centro and southern Mexico. In Oaxaca City 133 km to the south a person die of a hearth attack. No damages were reported. Most prominent moderate-sized earthquakes occurring in the southern Veracruz region since 1959 has been concentrated along two well defined seismic belts. One belt runs off the coast following nearly its contour. Here the earthquakes are shallow depth and mostly show a reverse fault mechanism. This belt of seismicity begins at the Los Tuxtlas volcanic field. Another seismic belt is located inland 70 km to the west. Here most earthquakes are of intermediate-depth (108-154 km) focus and normal faulting mechanism. The July 2014 earthquake is located near to this second seismic belt. In the present paper we discuss, within the regional geotectonic framework, the location and some aspects of the rupture process of the July 2014 earthquake.

  8. ENHANCEMENT OF THE 6.4 keV LINE IN THE INNER GALACTIC RIDGE: PROTON-INDUCED FLUORESCENCE?

    SciTech Connect

    Nobukawa, K. K.; Nobukawa, M.; Tsuru, T. G.; Tanaka, T.; Koyama, K.; Uchiyama, H.; Torii, K.; Fukui, Y.; Chernyshov, D. O.; Dogiel, V. A.

    2015-07-01

    A common idea for the origin of the Galactic diffuse X-ray emission, particularly that of the iron lines from neutral and highly ionized atoms, is a superposition of many cataclysmic variables and coronally active binaries. In this scenario, the flux should symmetrically distribute between the east and west on the plane with respect to Sagittarius A* because the stellar mass distribution determined by infrared observations is nearly symmetric. This symmetry is confirmed for the highly ionized iron line as well as the continuum emission. However, a clear excess of the neutral iron line in the near east of the Galactic center compared to the near-west side is found. The flux distribution of the excess emission well correlates with the molecular column density. The X-ray spectrum of the excess emission is described by a power-law continuum plus a 6.4 keV line with a large equivalent width of ∼1.3 keV, which is hardly explained by the low-energy electron bombardment scenario. The longitudinal and latitudinal distribution of the excess emission disfavors the X-ray irradiation, either by Sagittarius A* or by nearby X-ray binaries. Then, the low-energy proton bombardment is the most probable origin, although the high-energy density ∼80 eV cm{sup −3} in 0.1–1000 MeV is required and there is no conventional proton source in the vicinity.

  9. Final report on SIM.T-K6.4: Comparison of INMETRO and INTI humidity standards

    NASA Astrophysics Data System (ADS)

    Brionizio, J. D.; Skabar, J. G.

    2013-01-01

    The aim of this paper is to describe a bilateral comparison carried out by the hygrometry laboratories of the National Metrological Institutes of Brazil and Argentina, INMETRO and INTI, respectively. This comparison was planned and carried out as an informal comparison. But, in view of the lack of and the need for humidity comparison reports in the region of the Inter American Metrology System (SIM), we decided to register this comparison as a bilateral key comparison of the regional metrology organization (RMO), SIM.T-K6.4-INMETRO/INTI. The comparison was performed in the range from -20 ºC to +60 ºC dew/frost point temperatures in 20 °C steps. This paper presents the calibration methods of the laboratories, the uncertainty analysis and the comparison results. The measurements results of the comparison are also presented in terms of the normalised error (En) as a function of the dew/frost point temperature. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCT, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

  10. Structural Basis of Enzymatic Activity for the Ferulic Acid Decarboxylase (FADase) from Enterobacter sp. Px6-4

    PubMed Central

    Liang, Lianming; Sun, Yuna; Huang, Jingwen; Li, Xuemei; Cao, Yi; Meng, Zhaohui; Zhang, Ke-Qin

    2011-01-01

    Microbial ferulic acid decarboxylase (FADase) catalyzes the transformation of ferulic acid to 4-hydroxy-3-methoxystyrene (4-vinylguaiacol) via non-oxidative decarboxylation. Here we report the crystal structures of the Enterobacter sp. Px6-4 FADase and the enzyme in complex with substrate analogues. Our analyses revealed that FADase possessed a half-opened bottom β-barrel with the catalytic pocket located between the middle of the core β-barrel and the helical bottom. Its structure shared a high degree of similarity with members of the phenolic acid decarboxylase (PAD) superfamily. Structural analysis revealed that FADase catalyzed reactions by an “open-closed” mechanism involving a pocket of 8×8×15 Å dimension on the surface of the enzyme. The active pocket could directly contact the solvent and allow the substrate to enter when induced by substrate analogues. Site-directed mutagenesis showed that the E134A mutation decreased the enzyme activity by more than 60%, and Y21A and Y27A mutations abolished the enzyme activity completely. The combined structural and mutagenesis results suggest that during decarboxylation of ferulic acid by FADase, Trp25 and Tyr27 are required for the entering and proper orientation of the substrate while Glu134 and Asn23 participate in proton transfer. PMID:21283705

  11. A fundamental approach to adhesion: Synthesis, surface analysis, thermodynamics and mechanics. [acid-base properties of titanium 6-4 surfaces

    NASA Technical Reports Server (NTRS)

    Siriwardane, R.; Wightman, J. P.

    1980-01-01

    The acid-base properties of titanium 6-4 plates (low surface area) were investigated after three different pretreatments, namely Turco, phosphate-fluoride and Pasa-Jell. A series of indicators was used and color changes were detected using diffuse reflectance visible spectroscopy. Electron spectroscopy for chemical analysis was used to examine the indicator on the Ti 6-4 surface. Specular reflectance infra-red spectroscopy was used to study the adsorption of stearic acid from cyclohexane solutions on the Ti 6-4 surface.

  12. Fully quantal calculation of H2 translation-rotation states in (H2)4@5(12)6(4) clathrate sII inclusion compounds.

    PubMed

    Felker, Peter M

    2013-05-01

    The quantal translation-rotation (TR) states of the (p-H2)4@5(12)6(4) and (o-D2)4@5(12)6(4) hydrate clathrate sII inclusion compounds have been computed by nuclear-orbital/configuration-interaction methods. The model of these compounds in a rigid, high-symmetry 5(12)6(4) cage is treated in detail. The low-energy TR level structures of both isotopomers within this model are found to consist of states that can be readily described in terms of a small number of single-H2 and double-H2 excitation modes. The use of the high-symmetry results to facilitate the calculation and interpretation of (p-H2)4 and (o-D2)4 TR states in low-symmetry physically realizable 5(12)6(4) cages is also reported.

  13. Characterization of Arabidopsis photolyase enzymes and analysis of their role in protection from ultraviolet-B radiation.

    PubMed

    Waterworth, Wanda M; Jiang, Qing; West, Christopher E; Nikaido, M; Bray, Clifford M

    2002-05-01

    DNA photolyases are enzymes which mediate the light-dependent repair (photoreactivation) of UV-induced damage products in DNA by direct reversal of base damage rather than via excision repair pathways. Arabidopsis thaliana contains two photolyases specific for photoreactivation of either cyclobutane pyrimidine dimers (CPDs) or pyrimidine (6-4)pyrimidones (6-4PPs), the two major UV-B-induced photoproducts in DNA. Reduced FADH and a reduced pterin were identified as cofactors of the native Arabidopsis CPD photolyase protein. This is the first report of the chromophore composition of any native class II CPD photolyase protein to our knowledge. CPD photolyase protein levels vary between tissues and with leaf age and are highest in flowers and leaves of 3-5-week-old Arabidopsis plants. White light or UV-B irradiation induces CPD photolyase expression in Arabidopsis tissues. This contrasts with the 6-4PP photolyase protein which is constitutively expressed and not regulated by either white or UV-B light. Arabidopsis CPD and 6-4PP photolyase enzymes can remove UV-B-induced photoproducts from DNA in planta even when plants are grown under enhanced levels of UV-B irradiation and at elevated temperatures although the rate of removal of CPDs is slower at high growth temperatures. These studies indicate that Arabidopsis possesses the photorepair capacity to respond effectively to increased UV-B-induced DNA damage under conditions predicted to be representative of increases in UV-B irradiation levels at the Earth's surface and global warming in the twenty-first century.

  14. Genotoxic stress in plants: shedding light on DNA damage, repair and DNA repair helicases.

    PubMed

    Tuteja, Narendra; Ahmad, Parvaiz; Panda, Brahma B; Tuteja, Renu

    2009-01-01

    Plant cells are constantly exposed to environmental agents and endogenous processes that inflict damage to DNA and cause genotoxic stress, which can reduce plant genome stability, growth and productivity. Plants are most affected by solar UV-B radiation, which damage the DNA by inducing the formation of two main UV photoproducts such as cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6-4) pyrimidone photoproducts (6-4PPs). Reactive oxygen species (ROS) are also generated extra- or intra-cellularly, which constitute yet another source of genotoxic stress. As a result of this stress, the cellular DNA-damage responses (DDR) are activated, which transiently arrest the cell cycle and allow cells to repair DNA before proceeding into mitosis. DDR requires the activation of Ataxia telangiectasia-mutated (ATM) and Rad3-related (ATR) genes, which regulate the cell cycle and transmit the damage signals to downstream effectors of cell-cycle progression. Since genomic protection and stability are fundamental to ensure and sustain plant diversity and productivity, therefore, repair of DNA damages is essential. In plants the bulky DNA lesions, CPDs and 6-4PPs, are repaired by a simple and error-free mechanism: photoreactivation, which is a light-dependent mechanism and requires CPD or 6-4PP specific photolyases. In addition to this direct repair process, the plants also have sophisticated light-independent general repair mechanisms, such as the nucleotide excision repair (NER) and base excision repair (BER). The completed plant genome sequences reveal that most of the genes involved in NER and BER are present in higher plants, which suggests that the network of in-built DNA-damage repair mechanisms is conserved. This article describes the insight underlying the DNA damage and repair pathways in plants. The comet assay to measure the DNA damage and the role of DNA repair helicases such as XPD and XPB are also covered.

  15. The DDX6-4E-T interaction mediates translational repression and P-body assembly.

    PubMed

    Kamenska, Anastasiia; Simpson, Clare; Vindry, Caroline; Broomhead, Helen; Bénard, Marianne; Ernoult-Lange, Michèle; Lee, Benjamin P; Harries, Lorna W; Weil, Dominique; Standart, Nancy

    2016-07-27

    4E-Transporter binds eIF4E via its consensus sequence YXXXXLΦ, shared with eIF4G, and is a nucleocytoplasmic shuttling protein found enriched in P-(rocessing) bodies. 4E-T inhibits general protein synthesis by reducing available eIF4E levels. Recently, we showed that 4E-T bound to mRNA however represses its translation in an eIF4E-independent manner, and contributes to silencing of mRNAs targeted by miRNAs. Here, we address further the mechanism of translational repression by 4E-T by first identifying and delineating the interacting sites of its major partners by mass spectrometry and western blotting, including DDX6, UNR, unrip, PAT1B, LSM14A and CNOT4. Furthermore, we document novel binding between 4E-T partners including UNR-CNOT4 and unrip-LSM14A, altogether suggesting 4E-T nucleates a complex network of RNA-binding protein interactions. In functional assays, we demonstrate that joint deletion of two short conserved motifs that bind UNR and DDX6 relieves repression of 4E-T-bound mRNA, in part reliant on the 4E-T-DDX6-CNOT1 axis. We also show that the DDX6-4E-T interaction mediates miRNA-dependent translational repression and de novo P-body assembly, implying that translational repression and formation of new P-bodies are coupled processes. Altogether these findings considerably extend our understanding of the role of 4E-T in gene regulation, important in development and neurogenesis. PMID:27342281

  16. Heterogeneous stress field in the source area of the 2003 M6.4 Northern Miyagi Prefecture, NE Japan, earthquake

    NASA Astrophysics Data System (ADS)

    Yoshida, Keisuke; Hasegawa, Akira; Okada, Tomomi

    2016-07-01

    We investigated a detailed spatial distribution of principal stress axis orientations in the source area of the 2003 M6.4 Northern Miyagi Prefecture earthquake that occurred in the forearc of northeastern Japan. Aftershock hypocentres were precisely relocated by applying the double difference method to arrival time data obtained at temporary stations as well as at surrounding routine stations. We picked many P-wave polarity data from seismograms at these stations, which enabled us to obtain 312 well-determined focal mechanism solutions. Stress tensor inversions were performed by using these focal mechanism data. The results show that quite a lot of focal mechanisms are difficult to explain by the uniform stress field, especially near the large slip area of the main-shock rupture. Stress tensor inversions at the location of individual earthquakes show that σ1 axes are orientated mainly to WSW-ENE in the northern part of the source area, while they are oriented to NW-SE in the southern part. This spatial pattern is roughly similar to those of the static stress change by the main shock, which suggests that the observed spatially heterogeneous stress field was formed by the static stress change. If this is the case, the deviatoric stress magnitude before the main shock was very small. Another possibility is the heterogeneous stress field observed after the main shock had existed even before the main shock, although we do not know why it was formed. Unfavourable orientation of the main shock fault with respect to this stress field suggests that the fault is not strong in this case too.

  17. Enhanced UV-induced mutagenesis in the UV61 cell line, the Chinese hamster homologue of Cockayne's syndrome B, is associated with defective transcription coupled repair of cyclobutane pyrimidine dimers.

    PubMed

    Vreeswijk, M P; Overkamp, M W; Westland, B E; van Hees-Stuivenberg, S; Vrieling, H; Zdzienicka, M Z; van Zeeland, A A; Mullenders, L H

    1998-10-21

    Cells from Cockayne's syndrome (CS) patients are hypersensitive to the cytotoxic effects of UV-irradiation and are defective in transcription coupled repair (TCR). We have examined the mutagenic consequences of impaired TCR in the Chinese hamster cell line UV61, the rodent homologue of CS complementation group B. Analysis of the two major UV-induced photolesions, cyclobutane pyrimidine dimers (CPD) and pyrimidine 6-4 pyrimidone photoproducts (6-4 PP), revealed that repair of CPD from the transcribed strand was strongly reduced in UV61 cells, but repair of 6-4 PP was indistinguishable from that in wild-type hamster cells. UV-induced mutation induction was enhanced in UV61 compared to that observed in repair proficient cells. The spectrum of UV-induced base substitutions in UV61 was clearly different from that observed in wild-type hamster cells and resembled the spectrum previously observed in nucleotide excision repair deficient hamster cells. In UV61 cells a strong strand bias for mutation induction was found; assuming that premutagenic lesions occur at dipyrimidine sequences, 76% of the mutations could be attributed to lesions in the transcribed strand. These data strongly favour the hypothesis that defective TCR of CPD is responsible for the enhanced UV-induced mutagenesis in UV61 cells. PMID:9806502

  18. Auxiliary KCNE subunits modulate both homotetrameric Kv2.1 and heterotetrameric Kv2.1/Kv6.4 channels

    PubMed Central

    David, Jens-Peter; Stas, Jeroen I.; Schmitt, Nicole; Bocksteins, Elke

    2015-01-01

    The diversity of the voltage-gated K+ (Kv) channel subfamily Kv2 is increased by interactions with auxiliary β-subunits and by assembly with members of the modulatory so-called silent Kv subfamilies (Kv5-Kv6 and Kv8-Kv9). However, it has not yet been investigated whether these two types of modulating subunits can associate within and modify a single channel complex simultaneously. Here, we demonstrate that the transmembrane β-subunit KCNE5 modifies the Kv2.1/Kv6.4 current extensively, whereas KCNE2 and KCNE4 only exert minor effects. Co-expression of KCNE5 with Kv2.1 and Kv6.4 did not alter the Kv2.1/Kv6.4 current density but modulated the biophysical properties significantly; KCNE5 accelerated the activation, slowed the deactivation and steepened the slope of the voltage-dependence of the Kv2.1/Kv6.4 inactivation by accelerating recovery of the closed-state inactivation. In contrast, KCNE5 reduced the current density ~2-fold without affecting the biophysical properties of Kv2.1 homotetramers. Co-localization of Kv2.1, Kv6.4 and KCNE5 was demonstrated with immunocytochemistry and formation of Kv2.1/Kv6.4/KCNE5 and Kv2.1/KCNE5 complexes was confirmed by Fluorescence Resonance Energy Transfer experiments performed in HEK293 cells. These results suggest that a triple complex consisting of Kv2.1, Kv6.4 and KCNE5 subunits can be formed. In vivo, formation of such tripartite Kv2.1/Kv6.4/KCNE5 channel complexes might contribute to tissue-specific fine-tuning of excitability. PMID:26242757

  19. [Fe(CN)6]4- decorated mesoporous gelatin thin films for colorimetric detection and as sorbents of heavy metal ions.

    PubMed

    Shi, Li; Huang, Hubiao; Sun, Luwei; Lu, Yanping; Du, Binyang; Mao, Yiyin; Li, Junwei; Ye, Zhizhen; Peng, Xinsheng

    2013-09-28

    [Fe(CN)6](4-) decorated mesoporous gelatin films, acting as colorimetric sensors and sorbents for heavy metal ions, were prepared by incorporating [Fe(CN)6](4-) ions into the mesoporous gelatin films through electrostatic interaction. Gelatin-Prussian blue (PB) and gelatin-PB analogue composite films were successfully synthesized by immersing the [Fe(CN)6](4-) decorated gelatin films into aqueous solutions of metal ions, such as Fe(3+), Cu(2+), Co(2+), Pb(2+) and Cd(2+) (all as nitrates). The in situ formation process of PB or its analogues in the films was investigated using quartz crystal microbalance (QCM) measurements. According to the different colors of the PB nanoparticles and its analogues, the [Fe(CN)6](4-) decorated mesoporous gelatin films demonstrated colorimetric sensor abilities for detecting the corresponding metal ions by the naked eye with sufficient sensitivity at 1 ppm level and a quite short response time of 5 minutes. Moreover, due to the [Fe(CN)6](4-) functionality and other functional groups of gelatin itself, this [Fe(CN)6](4-) decorated mesoporous gelatin film shows a tens times higher adsorption ability for heavy metal ions in water than that of activated carbon. Due to both the efficient detection and high adsorption ability for heavy metal ions, this film has wide potential applications for the detection and purification of heavy metal ions from polluted water.

  20. [Fe(CN)6]4- decorated mesoporous gelatin thin films for colorimetric detection and as sorbents of heavy metal ions.

    PubMed

    Shi, Li; Huang, Hubiao; Sun, Luwei; Lu, Yanping; Du, Binyang; Mao, Yiyin; Li, Junwei; Ye, Zhizhen; Peng, Xinsheng

    2013-09-28

    [Fe(CN)6](4-) decorated mesoporous gelatin films, acting as colorimetric sensors and sorbents for heavy metal ions, were prepared by incorporating [Fe(CN)6](4-) ions into the mesoporous gelatin films through electrostatic interaction. Gelatin-Prussian blue (PB) and gelatin-PB analogue composite films were successfully synthesized by immersing the [Fe(CN)6](4-) decorated gelatin films into aqueous solutions of metal ions, such as Fe(3+), Cu(2+), Co(2+), Pb(2+) and Cd(2+) (all as nitrates). The in situ formation process of PB or its analogues in the films was investigated using quartz crystal microbalance (QCM) measurements. According to the different colors of the PB nanoparticles and its analogues, the [Fe(CN)6](4-) decorated mesoporous gelatin films demonstrated colorimetric sensor abilities for detecting the corresponding metal ions by the naked eye with sufficient sensitivity at 1 ppm level and a quite short response time of 5 minutes. Moreover, due to the [Fe(CN)6](4-) functionality and other functional groups of gelatin itself, this [Fe(CN)6](4-) decorated mesoporous gelatin film shows a tens times higher adsorption ability for heavy metal ions in water than that of activated carbon. Due to both the efficient detection and high adsorption ability for heavy metal ions, this film has wide potential applications for the detection and purification of heavy metal ions from polluted water. PMID:23887280

  1. Multi-wavelength diagnostics and modelling of the emission during a B6.4 flare of August 20, 2005

    NASA Astrophysics Data System (ADS)

    Awasthi, Arun Kumar; Berlicki, Arkadiusz; Rudawy, Powel; Heinzel, Petr

    2015-08-01

    We study the temporal, spatial and spectral evolution of multi-wavelength emission observed during a B6.4 flare occurred on August 20, 2005 with the motivation to outline the thermal and non-thermal processes during the precursor and gradual phase of the flare. Precursor phase is designated as the gradual enhancement of soft X-ray emission prior to onset of the impulsive phase. Observations from several space and ground based observatories viz. RHESSI, TRACE, GONG, SoHO/EIT and NoRP are included in this study. Temporal evolution of X-ray emission does not show the presence of hard X-rays (>12 keV) emission during the precursor phase of the flare. We synthesized X-ray images in 6-12 keV from RHESSI observations, which show several discrete sources during the precursor phase. Following to this, one of these sources pronounced during the main phase of the flare. We carry out in-depth analysis of chromospheric response in various phases of the flare employing high temporal cadence images of the Sun in Hα line centre as well as wings obtained from the Multi-channel Subtractive Double Pass Spectrograph (MSDP) at the Bialkow Observatory of the University of Wroclaw, Poland. Our analysis of Hα images during the main phase of the flare suggests localized emission in the form of kernels. On the contrary, we note extended and diffused source morphology of emitting region during the precursor phase of the flare. We also study various kinematic properties of different structures visible in the Hα images in the line centre as well as wings. In addition, the correlation of the relative timing of X-ray and Hα emission profile is performed to estimate the delay in the chromospheric response during different phases of flare. Further, we employ thermal plasma parameters estimated during the precursor and gradual phase to model the associated Hα emission. For the modeling we employ NLTE numerical codes modified for flare conditions. The modeled and observed flare emission

  2. Charmonium and e + e - pair photoproduction at mid-rapidity in ultra-peripheral Pb-Pb collisions at

    NASA Astrophysics Data System (ADS)

    Abbas, E.; Abelev, B.; Adam, J.; Adamová, D.; Adare, A. M.; Aggarwal, M. M.; Aglieri Rinella, G.; Agnello, M.; Agocs, A. G.; Agostinelli, A.; Ahammed, Z.; Ahmad, N.; Ahmad Masoodi, A.; Ahmed, I.; Ahn, S. A.; Ahn, S. U.; Aimo, I.; Ajaz, M.; Akindinov, A.; Aleksandrov, D.; Alessandro, B.; Alexandre, D.; Alici, A.; Alkin, A.; Almaráz Aviña, E.; Alme, J.; Alt, T.; Altini, V.; Altinpinar, S.; Altsybeev, I.; Andrei, C.; Andronic, A.; Anguelov, V.; Anielski, J.; Anson, C.; Antičić, T.; Antinori, F.; Antonioli, P.; Aphecetche, L.; Appelshäuser, H.; Arbor, N.; Arcelli, S.; Arend, A.; Armesto, N.; Arnaldi, R.; Aronsson, T.; Arsene, I. C.; Arslandok, M.; Asryan, A.; Augustinus, A.; Averbeck, R.; Awes, T. C.; Äystö, J.; Azmi, M. D.; Bach, M.; Badalà, A.; Baek, Y. W.; Bailhache, R.; Bala, R.; Baldisseri, A.; Baltasar Dos Santos Pedrosa, F.; Bán, J.; Baral, R. C.; Barbera, R.; Barile, F.; Barnaföldi, G. G.; Barnby, L. S.; Barret, V.; Bartke, J.; Basile, M.; Bastid, N.; Basu, S.; Bathen, B.; Batigne, G.; Batyunya, B.; Batzing, P. C.; Baumann, C.; Bearden, I. G.; Beck, H.; Behera, N. K.; Belikov, I.; Bellini, F.; Bellwied, R.; Belmont-Moreno, E.; Bencedi, G.; Beole, S.; Berceanu, I.; Bercuci, A.; Berdnikov, Y.; Berenyi, D.; Bergognon, A. A. E.; Bertens, R. A.; Berzano, D.; Betev, L.; Bhasin, A.; Bhati, A. K.; Bhom, J.; Bianchi, L.; Bianchi, N.; Bianchin, C.; Bielčík, J.; Bielčíková, J.; Bilandzic, A.; Bjelogrlic, S.; Blanco, F.; Blanco, F.; Blau, D.; Blume, C.; Boccioli, M.; Böttger, S.; Bogdanov, A.; Bøggild, H.; Bogolyubsky, M.; Boldizsár, L.; Bombara, M.; Book, J.; Borel, H.; Borissov, A.; Bossú, F.; Botje, M.; Botta, E.; Braidot, E.; Braun-Munzinger, P.; Bregant, M.; Breitner, T.; Broker, T. A.; Browning, T. A.; Broz, M.; Brun, R.; Bruna, E.; Bruno, G. E.; Budnikov, D.; Buesching, H.; Bufalino, S.; Buncic, P.; Busch, O.; Buthelezi, Z.; Caffarri, D.; Cai, X.; Caines, H.; Calvo Villar, E.; Camerini, P.; Canoa Roman, V.; Cara Romeo, G.; Carena, W.; Carena, F.; Carlin Filho, N.; Carminati, F.; Casanova Díaz, A.; Castillo Castellanos, J.; Castillo Hernandez, J. F.; Casula, E. A. R.; Catanescu, V.; Cavicchioli, C.; Ceballos Sanchez, C.; Cepila, J.; Cerello, P.; Chang, B.; Chapeland, S.; Charvet, J. L.; Chattopadhyay, S.; Chattopadhyay, S.; Cherney, M.; Cheshkov, C.; Cheynis, B.; Chibante Barroso, V.; Chinellato, D. D.; Chochula, P.; Chojnacki, M.; Choudhury, S.; Christakoglou, P.; Christensen, C. H.; Christiansen, P.; Chujo, T.; Chung, S. U.; Cicalo, C.; Cifarelli, L.; Cindolo, F.; Cleymans, J.; Colamaria, F.; Colella, D.; Collu, A.; Conesa Balbastre, G.; Conesa del Valle, Z.; Connors, M. E.; Contin, G.; Contreras, J. G.; Cormier, T. M.; Corrales Morales, Y.; Cortese, P.; Cortés Maldonado, I.; Cosentino, M. R.; Costa, F.; Cotallo, M. E.; Crescio, E.; Crochet, P.; Cruz Alaniz, E.; Cruz Albino, R.; Cuautle, E.; Cunqueiro, L.; Dainese, A.; Dang, R.; Danu, A.; Das, K.; Das, I.; Das, S.; Das, D.; Dash, S.; Dash, A.; De, S.; de Barros, G. O. V.; De Caro, A.; de Cataldo, G.; de Cuveland, J.; De Falco, A.; De Gruttola, D.; Delagrange, H.; Deloff, A.; De Marco, N.; Dénes, E.; De Pasquale, S.; Deppman, A.; D Erasmo, G.; de Rooij, R.; Diaz Corchero, M. A.; Di Bari, D.; Dietel, T.; Di Giglio, C.; Di Liberto, S.; Di Mauro, A.; Di Nezza, P.; Divià, R.; Djuvsland, Ø.; Dobrin, A.; Dobrowolski, T.; Dönigus, B.; Dordic, O.; Dubey, A. K.; Dubla, A.; Ducroux, L.; Dupieux, P.; Dutta Majumdar, A. K.; Elia, D.; Emschermann, D.; Engel, H.; Erazmus, B.; Erdal, H. A.; Eschweiler, D.; Espagnon, B.; Estienne, M.; Esumi, S.; Evans, D.; Evdokimov, S.; Eyyubova, G.; Fabris, D.; Faivre, J.; Falchieri, D.; Fantoni, A.; Fasel, M.; Fehlker, D.; Feldkamp, L.; Felea, D.; Feliciello, A.; Fenton-Olsen, B.; Feofilov, G.; Fernández Téllez, A.; Ferretti, A.; Festanti, A.; Figiel, J.; Figueredo, M. A. S.; Filchagin, S.; Finogeev, D.; Fionda, F. M.; Fiore, E. M.; Floratos, E.; Floris, M.; Foertsch, S.; Foka, P.; Fokin, S.; Fragiacomo, E.; Francescon, A.; Frankenfeld, U.; Fuchs, U.; Furget, C.; Fusco Girard, M.; Gaardhøje, J. J.; Gagliardi, M.; Gago, A.; Gallio, M.; Gangadharan, D. R.; Ganoti, P.; Garabatos, C.; Garcia-Solis, E.; Gargiulo, C.; Garishvili, I.; Gerhard, J.; Germain, M.; Geuna, C.; Gheata, M.; Gheata, A.; Ghidini, B.; Ghosh, P.; Gianotti, P.; Giubellino, P.; Gladysz-Dziadus, E.; Glässel, P.; Gomez, R.; Ferreiro, E. G.; González-Trueba, L. H.; González-Zamora, P.; Gorbunov, S.; Goswami, A.; Gotovac, S.; Graczykowski, L. K.; Grajcarek, R.; Grelli, A.; Grigoras, C.; Grigoras, A.; Grigoriev, V.; Grigoryan, A.; Grigoryan, S.; Grinyov, B.; Grion, N.; Gros, P.; Grosse-Oetringhaus, J. F.; Grossiord, J.-Y.; Grosso, R.; Guber, F.; Guernane, R.; Guerzoni, B.; Guilbaud, M.; Gulbrandsen, K.; Gulkanyan, H.; Gunji, T.; Gupta, A.; Gupta, R.; Haake, R.; Haaland, Ø.; Hadjidakis, C.; Haiduc, M.; Hamagaki, H.; Hamar, G.; Han, B. H.; Hanratty, L. D.; Hansen, A.; Harmanová-Tóthová, Z.; Harris, J. W.; Hartig, M.; Harton, A.; Hatzifotiadou, D.; Hayashi, S.; Hayrapetyan, A.; Heckel, S. T.; Heide, M.; Helstrup, H.; Herghelegiu, A.; Herrera Corral, G.; Herrmann, N.; Hess, B. A.; Hetland, K. F.; Hicks, B.; Hippolyte, B.; Hori, Y.; Hristov, P.; Hřivnáčová, I.; Huang, M.; Humanic, T. J.; Hwang, D. S.; Ichou, R.; Ilkaev, R.; Ilkiv, I.; Inaba, M.; Incani, E.; Innocenti, G. M.; Innocenti, P. G.; Ippolitov, M.; Irfan, M.; Ivan, C.; Ivanov, M.; Ivanov, A.; Ivanov, V.; Ivanytskyi, O.; Jachołkowski, A.; Jacobs, P. M.; Jahnke, C.; Jang, H. J.; Janik, M. A.; Jayarathna, P. H. S. Y.; Jena, S.; Jha, D. M.; Jimenez Bustamante, R. T.; Jones, P. G.; Jung, H.; Jusko, A.; Kaidalov, A. B.; Kalcher, S.; Kaliňák, P.; Kalliokoski, T.; Kalweit, A.; Kang, J. H.; Kaplin, V.; Kar, S.; Karasu Uysal, A.; Karavichev, O.; Karavicheva, T.; Karpechev, E.; Kazantsev, A.; Kebschull, U.; Keidel, R.; Ketzer, B.; Khan, M. M.; Khan, P.; Khan, S. A.; Khan, K. H.; Khanzadeev, A.; Kharlov, Y.; Kileng, B.; Kim, M.; Kim, T.; Kim, B.; Kim, S.; Kim, M.; Kim, D. J.; Kim, J. S.; Kim, J. H.; Kim, D. W.; Kirsch, S.; Kisel, I.; Kiselev, S.; Kisiel, A.; Klay, J. L.; Klein, J.; Klein-Bösing, C.; Kliemant, M.; Kluge, A.; Knichel, M. L.; Knospe, A. G.; Köhler, M. K.; Kollegger, T.; Kolojvari, A.; Kompaniets, M.; Kondratiev, V.; Kondratyeva, N.; Konevskikh, A.; Kovalenko, V.; Kowalski, M.; Kox, S.; Koyithatta Meethaleveedu, G.; Kral, J.; Králik, I.; Kramer, F.; Kravčáková, A.; Krelina, M.; Kretz, M.; Krivda, M.; Krizek, F.; Krus, M.; Kryshen, E.; Krzewicki, M.; Kucera, V.; Kucheriaev, Y.; Kugathasan, T.; Kuhn, C.; Kuijer, P. G.; Kulakov, I.; Kumar, J.; Kurashvili, P.; Kurepin, A.; Kurepin, A. B.; Kuryakin, A.; Kushpil, V.; Kushpil, S.; Kvaerno, H.; Kweon, M. J.; Kwon, Y.; Ladrón de Guevara, P.; Lagana Fernandes, C.; Lakomov, I.; Langoy, R.; La Pointe, S. L.; Lara, C.; Lardeux, A.; La Rocca, P.; Lea, R.; Lechman, M.; Lee, S. C.; Lee, G. R.; Legrand, I.; Lehnert, J.; Lemmon, R. C.; Lenhardt, M.; Lenti, V.; León, H.; Leoncino, M.; León Monzón, I.; Lévai, P.; Li, S.; Lien, J.; Lietava, R.; Lindal, S.; Lindenstruth, V.; Lippmann, C.; Lisa, M. A.; Ljunggren, H. M.; Lodato, D. F.; Loenne, P. I.; Loggins, V. R.; Loginov, V.; Lohner, D.; Loizides, C.; Loo, K. K.; Lopez, X.; López Torres, E.; Løvhøiden, G.; Lu, X.-G.; Luettig, P.; Lunardon, M.; Luo, J.; Luparello, G.; Luzzi, C.; Ma, R.; Ma, K.; Madagodahettige-Don, D. M.; Maevskaya, A.; Mager, M.; Mahapatra, D. P.; Maire, A.; Malaev, M.; Maldonado Cervantes, I.; Malinina, L.; Mal'Kevich, D.; Malzacher, P.; Mamonov, A.; Manceau, L.; Mangotra, L.; Manko, V.; Manso, F.; Manzari, V.; Mao, Y.; Marchisone, M.; Mareš, J.; Margagliotti, G. V.; Margotti, A.; Marín, A.; Markert, C.; Marquard, M.; Martashvili, I.; Martin, N. A.; Martinengo, P.; Martínez, M. I.; Martínez García, G.; Martynov, Y.; Mas, A.; Masciocchi, S.; Masera, M.; Masoni, A.; Massacrier, L.; Mastroserio, A.; Matyja, A.; Mayer, C.; Mazer, J.; Mazumder, R.; Mazzoni, M. A.; Meddi, F.; Menchaca-Rocha, A.; Mercado Pérez, J.; Meres, M.; Miake, Y.; Mikhaylov, K.; Milano, L.; Milosevic, J.; Mischke, A.; Mishra, A. N.; Miśkowiec, D.; Mitu, C.; Mizuno, S.; Mlynarz, J.; Mohanty, B.; Molnar, L.; Montaño Zetina, L.; Monteno, M.; Montes, E.; Moon, T.; Morando, M.; Moreira De Godoy, D. A.; Moretto, S.; Morreale, A.; Morsch, A.; Muccifora, V.; Mudnic, E.; Muhuri, S.; Mukherjee, M.; Müller, H.; Munhoz, M. G.; Murray, S.; Musa, L.; Musinsky, J.; Nandi, B. K.; Nania, R.; Nappi, E.; Nattrass, C.; Nayak, T. K.; Nazarenko, S.; Nedosekin, A.; Nicassio, M.; Niculescu, M.; Nielsen, B. S.; Niida, T.; Nikolaev, S.; Nikolic, V.; Nikulin, S.; Nikulin, V.; Nilsen, B. S.; Nilsson, M. S.; Noferini, F.; Nomokonov, P.; Nooren, G.; Nyanin, A.; Nyatha, A.; Nygaard, C.; Nystrand, J.; Ochirov, A.; Oeschler, H.; Oh, S.; Oh, S. K.; Oleniacz, J.; Oliveira Da Silva, A. C.; Onderwaater, J.; Oppedisano, C.; Ortiz Velasquez, A.; Oskarsson, A.; Ostrowski, P.; Otwinowski, J.; Oyama, K.; Ozawa, K.; Pachmayer, Y.; Pachr, M.; Padilla, F.; Pagano, P.; Paić, G.; Painke, F.; Pajares, C.; Pal, S. K.; Palaha, A.; Palmeri, A.; Papikyan, V.; Pappalardo, G. S.; Park, W. J.; Passfeld, A.; Patalakha, D. I.; Paticchio, V.; Paul, B.; Pavlinov, A.; Pawlak, T.; Peitzmann, T.; Pereira Da Costa, H.; Pereira De Oliveira Filho, E.; Peresunko, D.; Pérez Lara, C. E.; Perrino, D.; Peryt, W.; Pesci, A.; Pestov, Y.; Petráček, V.; Petran, M.; Petris, M.; Petrov, P.; Petrovici, M.; Petta, C.; Piano, S.; Pikna, M.; Pillot, P.; Pinazza, O.; Pinsky, L.; Pitz, N.; Piyarathna, D. B.; Planinic, M.; Płoskoń, M.; Pluta, J.; Pocheptsov, T.; Pochybova, S.; Podesta-Lerma, P. L. M.; Poghosyan, M. G.; Polák, K.; Polichtchouk, B.; Poljak, N.; Pop, A.; Porteboeuf-Houssais, S.; Pospíšil, V.; Potukuchi, B.; Prasad, S. K.; Preghenella, R.; Prino, F.; Pruneau, C. A.; Pshenichnov, I.; Puddu, G.; Punin, V.; Putschke, J.; Qvigstad, H.; Rachevski, A.; Rademakers, A.; Räihä, T. S.; Rak, J.; Rakotozafindrabe, A.; Ramello, L.; Raniwala, S.; Raniwala, R.; Räsänen, S. S.; Rascanu, B. T.; Rathee, D.; Rauch, W.; Rauf, A. W.; Razazi, V.; Read, K. F.; Real, J. S.; Redlich, K.; Reed, R. J.; Rehman, A.; Reichelt, P.; Reicher, M.; Renfordt, R.; Reolon, A. R.; Reshetin, A.; Rettig, F.; Revol, J.-P.; Reygers, K.; Riccati, L.; Ricci, R. A.; Richert, T.; Richter, M.; Riedler, P.; Riegler, W.; Riggi, F.; Rivetti, A.; Rodríguez Cahuantzi, M.; Rodriguez Manso, A.; Røed, K.; Rogochaya, E.; Rohr, D.; Röhrich, D.; Romita, R.; Ronchetti, F.; Rosnet, P.; Rossegger, S.; Rossi, A.; Roy, P.; Roy, C.; Rubio Montero, A. J.; Rui, R.; Russo, R.; Ryabinkin, E.; Rybicki, A.; Sadovsky, S.; Šafařík, K.; Sahoo, R.; Sahu, P. K.; Saini, J.; Sakaguchi, H.; Sakai, S.; Sakata, D.; Salgado, C. A.; Salzwedel, J.; Sambyal, S.; Samsonov, V.; Sanchez Castro, X.; Šándor, L.; Sandoval, A.; Sano, M.; Santagati, G.; Santoro, R.; Sarkamo, J.; Sarkar, D.; Scapparone, E.; Scarlassara, F.; Scharenberg, R. P.; Schiaua, C.; Schicker, R.; Schmidt, H. R.; Schmidt, C.; Schuchmann, S.; Schukraft, J.; Schuster, T.; Schutz, Y.; Schwarz, K.; Schweda, K.; Scioli, G.; Scomparin, E.; Scott, R.; Scott, P. A.; Segato, G.; Selyuzhenkov, I.; Senyukov, S.; Seo, J.; Serci, S.; Serradilla, E.; Sevcenco, A.; Shabetai, A.; Shabratova, G.; Shahoyan, R.; Sharma, S.; Sharma, N.; Rohni, S.; Shigaki, K.; Shtejer, K.; Sibiriak, Y.; Sicking, E.; Siddhanta, S.; Siemiarczuk, T.; Silvermyr, D.; Silvestre, C.; Simatovic, G.; Simonetti, G.; Singaraju, R.; Singh, R.; Singha, S.; Singhal, V.; Sinha, T.; Sinha, B. C.; Sitar, B.; Sitta, M.; Skaali, T. B.; Skjerdal, K.; Smakal, R.; Smirnov, N.; Snellings, R. J. M.; Søgaard, C.; Soltz, R.; Song, M.; Song, J.; Soos, C.; Soramel, F.; Sputowska, I.; Spyropoulou-Stassinaki, M.; Srivastava, B. K.; Stachel, J.; Stan, I.; Stefanek, G.; Steinpreis, M.; Stenlund, E.; Steyn, G.; Stiller, J. H.; Stocco, D.; Stolpovskiy, M.; Strmen, P.; Suaide, A. A. P.; Subieta Vásquez, M. A.; Sugitate, T.; Suire, C.; Suleymanov, M.; Sultanov, R.; Šumbera, M.; Susa, T.; Symons, T. J. M.; Szanto de Toledo, A.; Szarka, I.; Szczepankiewicz, A.; Szymański, M.; Takahashi, J.; Tangaro, M. A.; Tapia Takaki, J. D.; Tarantola Peloni, A.; Tarazona Martinez, A.; Tauro, A.; Tejeda Muñoz, G.; Telesca, A.; Ter Minasyan, A.; Terrevoli, C.; Thäder, J.; Thomas, D.; Tieulent, R.; Timmins, A. R.; Tlusty, D.; Toia, A.; Torii, H.; Toscano, L.; Trubnikov, V.; Truesdale, D.; Trzaska, W. H.; Tsuji, T.; Tumkin, A.; Turrisi, R.; Tveter, T. S.; Ulery, J.; Ullaland, K.; Ulrich, J.; Uras, A.; Urciuoli, G. M.; Usai, G. L.; Vajzer, M.; Vala, M.; Valencia Palomo, L.; Vallero, S.; Vande Vyvre, P.; Van Hoorne, J. W.; van Leeuwen, M.; Vannucci, L.; Vargas, A.; Varma, R.; Vasileiou, M.; Vasiliev, A.; Vechernin, V.; Veldhoen, M.; Venaruzzo, M.; Vercellin, E.; Vergara, S.; Vernet, R.; Verweij, M.; Vickovic, L.; Viesti, G.; Viinikainen, J.; Vilakazi, Z.; Villalobos Baillie, O.; Vinogradov, Y.; Vinogradov, L.; Vinogradov, A.; Virgili, T.; Viyogi, Y. P.; Vodopyanov, A.; Völkl, M. A.; Voloshin, S.; Voloshin, K.; Volpe, G.; von Haller, B.; Vorobyev, I.; Vranic, D.; Vrláková, J.; Vulpescu, B.; Vyushin, A.; Wagner, V.; Wagner, B.; Wan, R.; Wang, Y.; Wang, Y.; Wang, M.; Watanabe, K.; Weber, M.; Wessels, J. P.; Westerhoff, U.; Wiechula, J.; Wikne, J.; Wilde, M.; Wilk, G.; Williams, M. C. S.; Windelband, B.; Yaldo, C. G.; Yamaguchi, Y.; Yang, S.; Yang, P.; Yang, H.; Yasnopolskiy, S.; Yi, J.; Yin, Z.; Yoo, I.-K.; Yoon, J.; Yuan, X.; Yushmanov, I.; Zaccolo, V.; Zach, C.; Zampolli, C.; Zaporozhets, S.; Zarochentsev, A.; Závada, P.; Zaviyalov, N.; Zbroszczyk, H.; Zelnicek, P.; Zgura, I. S.; Zhalov, M.; Zhang, Y.; Zhang, H.; Zhang, X.; Zhou, D.; Zhou, Y.; Zhou, F.; Zhu, H.; Zhu, J.; Zhu, X.; Zhu, J.; Zichichi, A.; Zimmermann, A.; Zinovjev, G.; Zoccarato, Y.; Zynovyev, M.; Zyzak, M.

    2013-11-01

    The ALICE Collaboration at the LHC has measured the J/ ψ and ψ' photoproduction at mid-rapidity in ultra-peripheral Pb-Pb collisions at . The charmonium is identified via its leptonic decay for events where the hadronic activity is required to be minimal. The analysis is based on an event sample corresponding to an integrated luminosity of about 23 μb-1. The cross section for coherent and incoherent J/ ψ production in the rapidity interval -0.9< y<0.9, are and , respectively. The results are compared to theoretical models for J/ ψ production and the coherent cross section is found to be in good agreement with those models incorporating moderate nuclear gluon shadowing at Bjorken- x around 10-3, such as EPS09 parametrization. In addition the cross section for the process γγ→ e + e - has been measured and found to be in agreement with models implementing QED at leading order.

  3. Factors affecting the photoproduction of ammonia from dinitrogen and water by the cyanobacterium Anabaena sp. strain ATCC 33047

    SciTech Connect

    Ramos, J.L.; Guerrero, M.G.; Losada, M.

    1987-04-01

    Synthesis of ammonia from dinitrogen and water by suspensions of Anabaena sp. strain ATCC 33047 treated with the glutamine synthetase inhibitor L-methionine-D,L-sulfoximine is strictly dependent on light. Under otherwise optimal conditions, the yield of ammonia production is influenced by irradiance, as well as by the density, depth, and turbulence of the cell suspension. The interaction among these factors seems to determine the actual amount of light available to each single cell or filament in the suspension for the photoproduction process. Under convenient illumination, the limiting factor in the synthesis of ammonia seems to be the cellular nitrogenase activity level, but under limiting light conditions the limiting factor could, however, be the assimilatory power required for nitrogen fixation. Photosynthetic ammonia production from atmospheric nitrogen and water can operate with an efficiency of ca. 10% of its theoretical maximum, representing a remarkable process for the conversion of light energy into chemical energy.

  4. Lepton Universality Test in the Photoproduction of e-e+ Versus μ-μ+ Pairs on a Proton Target

    NASA Astrophysics Data System (ADS)

    Pauk, Vladyslav; Vanderhaeghen, Marc

    2015-11-01

    In view of the significantly different proton charge radius extracted from muonic hydrogen Lamb shift measurements as compared to electronic hydrogen spectroscopy or electron-scattering experiments, we study in this Letter the photoproduction of a lepton pair on a proton target in the limit of very small momentum transfer as a way to provide a test of the lepton universality when extracting the proton charge form factor. By detecting the recoiling proton in the γ p →l-l+p reaction, we show that a measurement of a ratio of e-e++μ-μ+ over e-e+ cross sections with an absolute precision of 7 ×1 0-4 would allow for a test to distinguish, at the 3 σ level, between the two different proton charge radii currently extracted from muonic and electronic observables.

  5. Continuous hydrogen photoproduction by Chlamydomonas reinhardtii: using a novel two-stage, sulfate-limited chemostat system.

    PubMed

    Fedorov, Alexander S; Kosourov, Sergey; Ghirardi, Maria L; Seibert, Michael

    2005-01-01

    This study demonstrates, for the first time, that it is possible to couple sulfate-limited Chlamydomonas reinhardtii growth to continuous H2 photoproduction for more than 4000 h. A two-stage chemostat system physically separates photosynthetic growth from H2 production, and it incorporates two automated photobioreactors (PhBRs). In the first PhBR, the algal cultures are grown aerobically in chemostat mode under limited sulfate to obtain photosynthetically competent cells. Active cells are then continuously delivered to the second PhBR, where H2 production occurs under anaerobic conditions. The dependence of the H2 production rate on sulfate concentration in the medium, dilution rates in the PhBRs, and incident light intensity is reported. PMID:15917617

  6. Measurement of {pi}{sup +}{pi}{sup -} Photoproduction in Double Polarization Experiments using the CLAS Spectrometer

    SciTech Connect

    Hanretty, Charles

    2007-10-26

    Spectroscopic predictions based on first principles are not possible in the non-perturbative regime of Quantum Chromodynamics (QCD) where the strong coupling constant is large. For this reason, effective theories and models have been developed to determine the masses, couplings, and decay widths of resonances. Various constituent quark models (CQMs) based on three quark degrees of freedom predict numerous baryon resonances that have not been experimentally verified and are thus 'missing'. The persistent non-observation of these states would present a big problem as the models would have failed to describe physical reality. CQMs predict strong couplings of these unobserved or missing states to {gamma}N as well as to N{eta}, N{eta}{sup '} or {delta}{pi}({delta}{yields}p{pi}) making photoproduction experiments a promising method to find these missing resonances. Previous analyses of unpolarized data show the importance of polarization obervables because some resonances reveal themselves more clearly in the interference with more dominant amplitudes. In addition, the determination of resonant contributions based on unpolarized data is not unique and requires further constraints provided by single- and double-polarization observables in the Partial Wave Analysis (PWA). A linearly- and circularly-polarized photon beam will be incident on a frozen-spin butanol target in Jefferson Lab's Hall B CLAS detector located in Newport News, Va. This detector allows the target to be polarized both longitudinally as well as transversely giving rise to measurable polarization observables in {pi}{sup +}{pi}{sup -} photoproduction. This experiment (FROST) will shed some light on the problem of the missing baryon resonances serving to better understand the properties of these states.

  7. The effects of ultraviolet light on aspects of DNA metabolism in cell lines derived from plodia interpunctella and Trichoplusia ni

    SciTech Connect

    Styer, S.C.

    1991-01-01

    Insect cells are significantly more resistant to the lethal effects of 254 nm ultraviolet light (UV) than mammalian cells. The predominant photoproduct produced by UV is the (5-6) cyclobutyl pyrimidine dimer. There is controversy whether this lesion, or another, the pyrimidine (6-4) pyrimidone, is responsible for the biological effects of UV. Insect cells contain a photolyase which selectively removes the (5-6), but not the (6-4) lesion, so that the relative roles of these lesions can be studied. Insect cell lines derived from the cabbage looper and the Indian meal moth were exposed to UV and analyzed for their ability to incorporate [sup 3]H-thymidine. After exposure, cells from the Indian meal moth exhibited a rapid and prolonged depression in the rate of thymidine incorporation, whereas cells from the cabbage looper showed only a slight drop in incorporation and a rapid recovery. The extent of depression in thymidine incorporation was not correlated to the amount of cell killing by UV in these cell lines. Blockage of fork progression was correlated to the depression in thymidine incorporation. Photoreactivation did not entirely relieve blockage, depression in thymidine incorporation or cell killing, indicating that although the (5-6) dimer appears to be the major lesion responsible for these effects, other lesions, such as the (6-4) photoproduct, may play a role. In addition, activation of alternative sites of replicon initiation appeared to correlate with the depression in thymidine incorporation and the excision capabilities in these cells. The resistance to UV in these insect cells compared to mammalian cells may be due to their ability to rapidly remove the (5-6) lesion, which is the critical lesion in these insect cells.

  8. Inhibition of semiconservative DNA synthesis in ICR 2A frog cells by pyrimidine dimers and nondimer photoproducts induced by ultraviolet radiation

    SciTech Connect

    Rosenstein, B.S.

    1984-11-01

    DNA synthesis was examined in ultraviolet (uv)-irradiated ICR 2A frog cells in which either pyrimidine dimers or nondimer photoproducts represented the major class of DNA lesions. In addition, cells were exposed to /sup 60/Co ..gamma.. rays. The cultures were pulse-labeled and the size distribution of the DNA synthesized was estimated using both sucrose gradient sedimentation and alkaline step elution. Using either of these techniques, it was found that the presence of dimers resulted in a reduction principally in the synthesis of high molecular weight (MW) DNA. In contrast, nondimer photoproducts caused a strong inhibition in the synthesis of low MW DNA, as was also observed in ..gamma..-irradiated cells. Hence the induction of pyrimidine dimers in DNA mainly affected the elongation of replicons, whereas nondimer lesions primarily caused an inhibition of replicon initiation.

  9. Integration and scaling of UV-B radiation effects on plants: from DNA to leaf

    PubMed Central

    Suchar, Vasile Alexandru; Robberecht, Ronald

    2015-01-01

    A process-based model integrating the effects of UV-B radiation through epidermis, cellular DNA, and its consequences to the leaf expansion was developed from key parameters in the published literature. Enhanced UV-B radiation-induced DNA damage significantly delayed cell division, resulting in significant reductions in leaf growth and development. Ambient UV-B radiation-induced DNA damage significantly reduced the leaf growth of species with high relative epidermal absorbance at longer wavelengths and average/low pyrimidine cyclobutane dimers (CPD) photorepair rates. Leaf expansion was highly dependent on the number of CPD present in the DNA, as a result of UV-B radiation dose, quantitative and qualitative absorptive properties of epidermal pigments, and repair mechanisms. Formation of pyrimidine-pyrimidone (6-4) photoproducts (6-4PP) has no effect on the leaf expansion. Repair mechanisms could not solely prevent the UV-B radiation interference with the cell division. Avoidance or effective shielding by increased or modified qualitative epidermal absorptance was required. Sustained increased UV-B radiation levels are more detrimental than short, high doses of UV-B radiation. The combination of low temperature and increased UV-B radiation was more significant in the level of UV-B radiation-induced damage than UV-B radiation alone. Slow-growing leaves were more affected by increased UV-B radiation than fast-growing leaves. PMID:26257869

  10. Regulation of plant MSH2 and MSH6 genes in the UV-B-induced DNA damage response.

    PubMed

    Lario, Luciana D; Ramirez-Parra, Elena; Gutierrez, Crisanto; Casati, Paula; Spampinato, Claudia P

    2011-05-01

    Deleterious effects of UV-B radiation on DNA include the formation of cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6-4) pyrimidone photoproducts (6-4PPs). These lesions must be repaired to maintain the integrity of DNA and provide genetic stability. Of the several repair systems involved in the recognition and removal of UV-B-induced lesions in DNA, the focus in the present study was on the mismatch repair system (MMR). The contribution of MutSα (MSH2-MSH6) to UV-induced DNA lesion repair and cell cycle regulation was investigated. MSH2 and MSH6 genes in Arabidopsis and maize are up-regulated by UV-B, indicating that MMR may have a role in UV-B-induced DNA damage responses. Analysis of promoter sequences identified MSH6 as a target of the E2F transcription factors. Using electrophoretic mobility shift assays, MSH6 was experimentally validated as an E2F target gene, suggesting an interaction between MMR genes and the cell cycle control. Mutations in MSH2 or MSH6 caused an increased accumulation of CPDs relative to wild-type plants. In addition, msh2 mutant plants showed a different expression pattern of cell cycle marker genes after the UV-B treatment when compared with wild-type plants. Taken together, these data provide evidence that plant MutSα is involved in a UV-B-induced DNA damage response pathway.

  11. Integration and scaling of UV-B radiation effects on plants: from DNA to leaf.

    PubMed

    Suchar, Vasile Alexandru; Robberecht, Ronald

    2015-07-01

    A process-based model integrating the effects of UV-B radiation through epidermis, cellular DNA, and its consequences to the leaf expansion was developed from key parameters in the published literature. Enhanced UV-B radiation-induced DNA damage significantly delayed cell division, resulting in significant reductions in leaf growth and development. Ambient UV-B radiation-induced DNA damage significantly reduced the leaf growth of species with high relative epidermal absorbance at longer wavelengths and average/low pyrimidine cyclobutane dimers (CPD) photorepair rates. Leaf expansion was highly dependent on the number of CPD present in the DNA, as a result of UV-B radiation dose, quantitative and qualitative absorptive properties of epidermal pigments, and repair mechanisms. Formation of pyrimidine-pyrimidone (6-4) photoproducts (6-4PP) has no effect on the leaf expansion. Repair mechanisms could not solely prevent the UV-B radiation interference with the cell division. Avoidance or effective shielding by increased or modified qualitative epidermal absorptance was required. Sustained increased UV-B radiation levels are more detrimental than short, high doses of UV-B radiation. The combination of low temperature and increased UV-B radiation was more significant in the level of UV-B radiation-induced damage than UV-B radiation alone. Slow-growing leaves were more affected by increased UV-B radiation than fast-growing leaves. PMID:26257869

  12. Seasonal and spatial variabilities in the water chemistry of prairie pothole wetlands influence the photoproduction of reactive intermediates.

    PubMed

    McCabe, Andrew J; Arnold, William A

    2016-07-01

    The hydrology and water chemistry of prairie pothole wetlands vary spatially and temporally, on annual and decadal timescales. Pesticide contamination of wetlands arising from agricultural activities is a foremost concern. Photochemical reactions are important in the natural attenuation of pesticides and may be important in limiting ecological and human exposure. Little is known, however, about the variable influence of wetland water chemistry on indirect photochemistry. In this study, seasonal water samples were collected from seven sites throughout the prairie pothole region over three years to understand the spatiotemporal dynamics of reactive intermediate photoproduction. Samples were classified by the season in which they were collected (spring, summer, or fall) and the typical hydroperiod of the wetland surface water (temporary or semi-permanent). Under photostable conditions, steady-state concentrations and apparent quantum yields or quantum yield coefficients were measured for triplet excited states of dissolved organic matter, singlet oxygen, hydroxyl radical, and carbonate radical under simulated sunlight. Steady-state concentrations and quantum yields increased on average by 15% and 40% from spring to fall, respectively. Temporary wetlands had 40% higher steady-state concentrations of reactive intermediates than semi-permanent wetlands, but 50% lower quantum yields. Computed quantum yields for reactive intermediate formation were used to predict the indirect photochemical half-lives of seven pesticides in average temporary and semi-permanent prairie pothole wetlands. As a first approximation, the predictions agree to within two orders of magnitude of previously reported half-lives. PMID:27174849

  13. Two-Photon Absorption of Bacteriorhodopsin: Formation of a Red-Shifted Thermally Stable Photoproduct F620

    PubMed Central

    Fischer, Thorsten; Hampp, Norbert A.

    2005-01-01

    By means of high-intensity 532 nm laser pulses, a photochemical conversion of the initial B570 state of bacteriorhodopsin (BR) to a stable photoproduct absorbing maximally at ≈620 nm in BR suspensions and at ≈610 nm in BR films is induced. This state, which we named F620, is photochemically further converted to a group of three products with maximal absorptions in the wavelength range from 340 nm to 380 nm, which show identical spectral properties to the so-called P360 state reported in the literature. The photoconversion from B570 to F620 is most likely a resonant two-photon absorption induced step. The formation of F620 and P360 leads to a distinguished photo-induced permanent optical anisotropy in BR films. The spectral dependence of the photo-induced anisotropy and the anisotropy orientations at the educt (B570) and product (F620) wavelengths are strong indicators that F620 is formed in a direct photochemical step from B570. The chemical nature of the P360 products probably is that of a retro-retinal containing BR, but the structural characteristics of the F620 state are still unclear. The photo-induced permanent anisotropy induced by short laser pulses in BR films helps to better understand the photochemical pathways related to this transition, and it is interesting in view of potential applications as this feature is the molecular basis for permanent optical data storage using BR films. PMID:15894635

  14. Polarization components in π0 photoproduction at photon energies up to 5.6 GeV

    SciTech Connect

    Luo, W.; Brash, E. J.; Gilman, R.; Jones, M. K.; Meziane, M.; Pentchev, L.; Perdrisat, C. F; Puckett, A. J.R.; Punjabi, V.; Wesselmann, F. R.; Marsh, A.; Matulenko, Y.; Maxwell, J.; Meekins, D.; Melnik, Y.; Miller, J.; Mkrtchyan, A.; Mkrtchyan, H.; Moffit, B.; Moreno, O.; Mulholland, J.; Narayan, A.; Nuruzzaman, .; Nedev, S.; Piasetzky, E.; Pierce, W.; Piskunov, N. M.; Prok, Y.; Ransome, R. D.; Razin, D. S.; Reimer, P. E.; Reinhold, J.; Rondon, O.; Shabestari, M.; Shahinyan, A.; Shestermanov, K.; Sirca, S.; Sitnik, I.; Smykov, L.; Smith, G.; Solovyev, L.; Solvignon, P.; Strakovsky, I. I; Subedi, R.; Suleiman, R.; Tomasi-Gustafsson, E.; Vasiliev, A.; Veilleux, M.; Wood, S.; Ye, Z.; Zanevsky, Y.; Zhang, X.; Zhang, Y.; Zheng, X.; Zhu, L.; Ahmidouch, A.; Albayrak, I.; Aniol, K. A.; Arrington, J.; Asaturyan, A.; Ates, O.; Baghdasaryan, H.; Benmokhtar, F.; Bertozzi, W.; Bimbot, L.; Bosted, P.; Boeglin, W.; Butuceanu, C.; Carter, P.; Chernenko, S.; Christy, M. E.; Commisso, M.; Cornejo, J. C.; Covrig, S.; Danagoulian, S.; Daniel, A.; Davidenko, A.; Day, D.; Dhamija, S.; Dutta, D.; Ent, R.; Frullani, S.; Fenker, H.; Frlez, E.; Garibaldi, F.; Gaskell, D.; Gilad, S.; Goncharenko, Y.; Hafidi, K.; Hamilton, D.; Higinbotham, D. W.; Hinton, W.; Horn, T.; Hu, B.; Huang, J.; Huber, G. M.; Jensen, E.; Kang, H.; Keppel, C.; Khandaker, M.; King, P.; Kirillov, D.; Kohl, M.; Kravtsov, V.; Kumbartzki, G.; Li, Y.; Mamyan, V.; Margaziotis, D. J.; Markowitz, P.

    2012-05-31

    We present new data for the polarization observables of the final state proton in the 1H(→ γ, → p)π0 reaction. These data can be used to test predictions based on hadron helicity conservation (HHC) and perturbative QCD (pQCD). These data have both small statistical and systematic uncertainties, and were obtained with beam energies between 1.8 and 5.6 GeV and for π0 scattering angles larger than 75{sup o} in center-of-mass (c.m.) frame. The data extend the polarization measurements data base for neutral pion photoproduction up to Eγ = 5.6 GeV. The results show non-zero induced polarization above the resonance region. The polarization transfer components vary rapidly with the photon energy and π0 scattering angle in the center-of-mass frame. This indicates that HHC does not hold and that the pQCD limit is still not reached in the energy regime of this experiment.

  15. Polarization components in π0 photoproduction at photon energies up to 5.6 GeV

    DOE PAGESBeta

    Luo, W.; Brash, E. J.; Gilman, R.; Jones, M. K.; Meziane, M.; Pentchev, L.; Perdrisat, C. F; Puckett, A. J.R.; Punjabi, V.; Wesselmann, F. R.; et al

    2012-05-31

    We present new data for the polarization observables of the final state proton in the 1H(→ γ, → p)π0 reaction. These data can be used to test predictions based on hadron helicity conservation (HHC) and perturbative QCD (pQCD). These data have both small statistical and systematic uncertainties, and were obtained with beam energies between 1.8 and 5.6 GeV and for π0 scattering angles larger than 75{sup o} in center-of-mass (c.m.) frame. The data extend the polarization measurements data base for neutral pion photoproduction up to Eγ = 5.6 GeV. The results show non-zero induced polarization above the resonance region. Themore » polarization transfer components vary rapidly with the photon energy and π0 scattering angle in the center-of-mass frame. This indicates that HHC does not hold and that the pQCD limit is still not reached in the energy regime of this experiment.« less

  16. Theoretical study of photoproduction of an η'N bound state on a deuteron target with forward proton emission

    NASA Astrophysics Data System (ADS)

    Sekihara, Takayasu; Sakai, Shuntaro; Jido, Daisuke

    2016-08-01

    Possibilities of observing a signal of an η'n bound state are investigated by considering photoproductions of the η and η' mesons on a deuteron target with forward proton emission. For this purpose, we take the η'n interaction from the linear σ model with a coupling to η n , in which an s -wave η'n bound state can be dynamically generated, and we fix the γ p →η p and η'p scattering amplitudes so as to reproduce the experimental cross sections with forward proton emission. By using these γ p →η(')p and η(')n →η(')n amplitudes, we calculate cross sections of the γ d →η n p and η'n p reactions with forward proton emission in single and η(')-exchange double-scattering processes. As a result, we find that the signal of the η'n bound state can be seen below the η'n threshold in the η n invariant mass spectrum of the γ d →η n p reaction and is comparable with the contribution from the quasifree η' production above the η'n threshold. We also discuss the behavior of the signal of the η'n bound state in several experimental conditions and model parameters.

  17. Rapidity and momentum transfer distributions of coherent J/ψ photoproduction in ultraperipheral pPb collisions at the LHC

    NASA Astrophysics Data System (ADS)

    Guzey, V.; Zhalov, M.

    2014-02-01

    Based on accurate calculations of the flux of equivalent photons of the proton and heavy nuclei and the pQCD framework for the gluon distribution in the proton and nuclei, we analyze the rapidity and momentum transfer distributions of coherent J/ψ photoproduction in ultraperipheral proton-Pb collisions at the LHC. We demonstrate that unlike the case of proton-proton UPCs marred by certain theoretical uncertainties and experimental limitations, after a cut excluding the region of small momentum transfers, ultraperipheral proton-Pb collisions offer a clean way to study the gluon distribution in the proton down to x ≈ 10-5. Our analysis of the momentum transfer distributions shows that an interplay of J/ψ production by low-energy photons on the nucleus and by high-energy photons on the proton in proton-Pb UPCs can result in some excess of events at small p t in a definite region of the rapidity y.

  18. Coherent π{sup 0}-photoproduction on the deuteron near the η-production threshold including polarization observables

    SciTech Connect

    Darwish, Eed M.; Al-Thoyaib, Suleiman S.

    2014-12-15

    Coherent π{sup 0}-photoproduction on the deuteron including polarization observables is studied in the energy region near the η-production threshold at backward center-of-mass angles of the outgoing pion. This work is motivated by the measurements of the CLAS Collaboration at Jefferson Lab, where a cusp-like structure in the energy dependence of the differential cross section has been observed at extremely backward pion angles. The present approach is based on the impulse approximation and first-order rescattering diagrams with intermediate production of both π- and η-mesons. Numerical results for unpolarized cross sections, the linear photon asymmetry (Σ), the vector (T{sub 11}) and tensor (T{sub 2M}, M=0, 1, 2) deuteron target asymmetries, and the double polarization E-asymmetry are predicted and compared with available experimental data and other theoretical models. The effect of first-order rescattering is found to be much larger in spin asymmetries than in the unpolarized cross sections. It reaches on average about 40% in the tensor target and E asymmetries. Compared to the experimental data from CLAS Collaboration, sizable discrepancies are found. This is not the case for the linear photon asymmetry, for which a better comparison with the data from YerPhI Collaboration is obtained.

  19. Photoproduction of lepton pairs in proton-nucleus and nucleus-nucleus collisions at RHIC and LHC energies

    SciTech Connect

    Moreira, B. D.; Goncalves, V. P.; De Santana Amaral, J. T.

    2013-03-25

    In this contribution we study coherent interactions as a probe of the nonlinear effects in the Quantum Electrodynamics (QED). In particular, we study the multiphoton effects in the production of leptons pairs for proton-nucleus and nucleus-nucleus collisions for heavy nuclei. In the proton-nucleus we assume the ultrarelativistic proton as a source of photons and estimate the photoproduction of lepton pairs on nuclei at RHIC and LHC energies considering the multiphoton effects associated to multiple rescattering of the projectile photon on the proton of the nucleus. In nucleus - nucleus colllisions we consider the two nuclei as a source of photons. As each scattering contributes with a factor {alpha}Z to the cross section, this contribution must be taken into account for heavy nuclei. We consider the Coulomb corrections to calculate themultiple scatterings and estimate the total cross section for muon and tau pair production in proton-nucleus and nucleus-nucleus collisions at RHIC and LHC energies.

  20. In situ photoproduction of dichlorodibenzo- p-dioxin from non-ionic triclosan isolated in solid argon

    NASA Astrophysics Data System (ADS)

    Kuş, Nihal; Reva, Igor; Bayarı, Sevgi; Fausto, Rui

    2012-01-01

    The infrared spectrum of triclosan [or 5-chloro-2-(2,4-dichlorophenoxy)phenol] isolated in a low temperature (˜15 K) argon matrix has been recorded and assigned with help of DFT claculations undertaken with the B3LYP functional and the 6-311++G(d,p) basis set. The obtained spectrum doubtlessly exhibits the characteristic vibrational signature of the neutral (phenol) form of the compound, which exists in two different conformations (forms I and II) in the matrix, in a I: II population ratio of ca. 0.75. Upon broadband UV irradiation of the matrix-isolated triclosan with unfiltered light provided by a xenon arc lamp, formation of 2,8-dichlorodibenzo- p-dioxin (2,8-DCDD) was observed, together with HCl. The reaction seems to occur through initial photoproduction of the triclosan phenol radical derivative, and involve in the initial step participation of dissociative (πσ ∗) excited states along the OH stretching coordinate, as observed previously for other phenol derivatives. The photochemically detached hydrogen atom derived from triclosan may then react with the closest located chlorine atom in the triclosan molecule to yield HCl and a biradical species, which can subsequently undergo a ring-closure reaction by intramolecular recombination, leading to the observed 2,8-DCDD.

  1. Hydrogen photoproduction by nutrient-deprived Chlamydomonas reinhardtii cells immobilized within thin alginate films under aerobic and anaerobic conditions.

    PubMed

    Kosourov, Sergey N; Seibert, Michael

    2009-01-01

    A new technique for immobilizing H2-photoproducing green algae within a thin (<400 microm) alginate film has been developed. Alginate films with entrapped sulfur/phosphorus-deprived Chlamydomonas reinhardtii, strain cc124, cells demonstrate (a) higher cell density (up to 2,000 microg Chl mL(-1) of matrix), (b) kinetics of H2 photoproduction similar to sulfur-deprived suspension cultures, (c) higher specific rates (up to 12.5 micromol mg(-1) Chl h(-1)) of H2 evolution, (d) light conversion efficiencies to H2 of over 1% and (e) unexpectedly high resistance of the H2-photoproducing system to inactivation by atmospheric O2. The algal cells, entrapped in alginate and then placed in vials containing 21% O2 in the headspace, evolved up to 67% of the H2 gas produced under anaerobic conditions. The results indicate that the lower susceptibility of the immobilized algal H2-producing system to inactivation by O2 depends on two factors: (a) the presence of acetate in the medium, which supports higher rates of respiration and (b) the capability of the alginate polymer itself to effectively separate the entrapped cells from O2 in the liquid and headspace and restrict O2 diffusion into the matrix. The strategy presented for immobilizing algal cells within thin polymeric matrices shows the potential for scale-up and possible future applications. PMID:18823051

  2. Mesostructured material based on [Re6Te8(CN)6]4- cluster and Mn(2+): a rational synthesis of hexagonal nonoxidic mesoscale material.

    PubMed

    Vien, Vo; Suh, Min-Jung; Huh, Seong; Kim, Youngmee; Kim, Sung-Jin

    2009-02-01

    A new nonoxidic mesostructured material with highly ordered hexagonal symmetry, [C(16)H(33)N(CH(3))(3)](3)Mn(0.5)[Re(6)Te(8)(CN)(6)], has been obtained through supramolecular assembly of [Re(6)Te(8)(CN)(6)](4-) clusters in the presence of a transition metal cation, Mn(2+), with cetyltrimethylammonium bromide as a liquid crystal template under hydrothermal conditions.

  3. Mesostructured material based on [Re6Te8(CN)6]4- cluster and Mn(2+): a rational synthesis of hexagonal nonoxidic mesoscale material.

    PubMed

    Vien, Vo; Suh, Min-Jung; Huh, Seong; Kim, Youngmee; Kim, Sung-Jin

    2009-02-01

    A new nonoxidic mesostructured material with highly ordered hexagonal symmetry, [C(16)H(33)N(CH(3))(3)](3)Mn(0.5)[Re(6)Te(8)(CN)(6)], has been obtained through supramolecular assembly of [Re(6)Te(8)(CN)(6)](4-) clusters in the presence of a transition metal cation, Mn(2+), with cetyltrimethylammonium bromide as a liquid crystal template under hydrothermal conditions. PMID:19283284

  4. Construction of Azabicyclo[6.4.0]dodecatrienes Based on Rhodium(I)-Catalyzed Intramolecular [6+2] Cycloaddition between Azetidine, Allene, and Alkynes.

    PubMed

    Yasuda, Shigeo; Yokosawa, Haruna; Mukai, Chisato

    2016-01-01

    Treatment of the allenylazetidine-alkynes with a catalytic amount of [RhCl(CO)dppp]2 (dppp: 1,3-bis(diphenylphosphino)propane) effected the intramolecular hetero-[6+2]-type ring-closing reaction via the C-C bond cleavage of the azetidine ring to produce azabicyclo[6.4.0]dodecatriene derivatives in good to excellent yields. The formation of the oxa analogue could also be achieved.

  5. 40 CFR 180.437 - Methyl 2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl)-p-toluate and methyl 6-(4-isopropyl-4...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Methyl 2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl)-p-toluate and methyl 6-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl)-m-toluate; tolerances for residues. 180.437 Section 180.437 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS...

  6. Photoaffinity derivative of colchicine: 6'-(4'-azido-2'-nitrophenylamino)hexanoyldeacetylcolchicine. Photolabeling and location of the colchicine-binding site on the alpha-subunit of tubulin

    SciTech Connect

    Williams, R.F.; Mumford, C.L.; Williams, G.A.; Floyd, L.J.; Aivaliotis, M.J.; Martinez, R.A.; Robinson, A.K.; Barnes, L.D.

    1985-11-05

    A photoaffinity analog of colchicine, 6-(4'-azido-2'-nitrophenylamino)hexanoyldeacetylcolchicine, was synthesized by reacting deacetylcolchicine or (TH)deacetylcochicine with N-succinimidyl-6-(4'-azido-2'-nitrophenylamino)hexanoate. Homogeneity of the photoaffinity analog was established by thin-layer chromatography and high-pressure liquid chromatography. The structure of the photoaffinity analog was determined by H and TC NMR, infrared and ultraviolet-visible spectroscopies, and elemental analysis. Binding of 6-(4'-azido-2'-nitrophenylamino)hexanoyldeacetylcolchicine to bovine renal tubulin was measured by competition with (TH)colchicine. The value of the apparent Ki for the photoaffinity analog was 0.28 microM in the concentration range of 0.8-1.2 microM of the analog. A value of 0.50 microM for the apparent Kd was measured by the direct binding of the tritiated photoaffinity analog to tubulin. The analog is slightly more potent an inhibitor of microtubule formation than colchicine. The photoaffinity analog reacted with renal tubulin upon irradiation with a mercury lamp equipped with a 420-nm cutoff filter. Spectral and radiochemical analyses of the tubulin after photolysis and dialysis have demonstrated a stoichiometric incorporation of the photoaffinity analog in the alpha-subunit of the tubulin. Covalent labeling of tubulin with the photoaffinity analog decreases the extent of (TH)colchicine binding by more than 90%.

  7. THE ORIGIN OF THE 6.4 keV LINE EMISSION AND H{sub 2} IONIZATION IN THE DIFFUSE MOLECULAR GAS OF THE GALACTIC CENTER REGION

    SciTech Connect

    Dogiel, V. A.; Chernyshov, D. O.; Tatischeff, V.; Terrier, R.

    2013-07-10

    We investigate the origin of the diffuse 6.4 keV line emission recently detected by Suzaku and the source of H{sub 2} ionization in the diffuse molecular gas of the Galactic center (GC) region. We show that Fe atoms and H{sub 2} molecules in the diffuse interstellar medium of the GC are not ionized by the same particles. The Fe atoms are most likely ionized by X-ray photons emitted by Sgr A* during a previous period of flaring activity of the supermassive black hole. The measured longitudinal intensity distribution of the diffuse 6.4 keV line emission is best explained if the past activity of Sgr A* lasted at least several hundred years and released a mean 2-100 keV luminosity {approx}> 10{sup 38} erg s{sup -1}. The H{sub 2} molecules of the diffuse gas cannot be ionized by photons from Sgr A*, because soft photons are strongly absorbed in the interstellar gas around the central black hole. The molecular hydrogen in the GC region is most likely ionized by low-energy cosmic rays, probably protons rather than electrons, whose contribution into the diffuse 6.4 keV line emission is negligible.

  8. Enhancement of the catalytic activity of ferulic acid decarboxylase from Enterobacter sp. Px6-4 through random and site-directed mutagenesis.

    PubMed

    Lee, Hyunji; Park, Jiyoung; Jung, Chaewon; Han, Dongfei; Seo, Jiyoung; Ahn, Joong-Hoon; Chong, Youhoon; Hur, Hor-Gil

    2015-11-01

    The enzyme ferulic acid decarboxylase (FADase) from Enterobacter sp. Px6-4 catalyzes the decarboxylation reaction of lignin monomers and phenolic compounds such as p-coumaric acid, caffeic acid, and ferulic acid into their corresponding 4-vinyl derivatives, that is, 4-vinylphenol, 4-vinylcatechol, and 4-vinylguaiacol, respectively. Among various ferulic acid decarboxylase enzymes, we chose the FADase from Enterobacter sp. Px6-4, whose crystal structure is known, and produced mutants to enhance its catalytic activity by random and site-directed mutagenesis. After three rounds of sequential mutations, FADase(F95L/D112N/V151I) showed approximately 34-fold higher catalytic activity than wild-type for the production of 4-vinylguaiacol from ferulic acid. Docking analyses suggested that the increased activity of FADase(F95L/D112N/V151I) could be due to formation of compact active site compared with that of the wild-type FADase. Considering the amount of phenolic compounds such as lignin monomers in the biomass components, successfully bioengineered FADase(F95L/D112N/V151I) from Enterobacter sp. Px6-4 could provide an ecofriendly biocatalytic tool for producing diverse styrene derivatives from biomass.

  9. Enhancement of the catalytic activity of ferulic acid decarboxylase from Enterobacter sp. Px6-4 through random and site-directed mutagenesis.

    PubMed

    Lee, Hyunji; Park, Jiyoung; Jung, Chaewon; Han, Dongfei; Seo, Jiyoung; Ahn, Joong-Hoon; Chong, Youhoon; Hur, Hor-Gil

    2015-11-01

    The enzyme ferulic acid decarboxylase (FADase) from Enterobacter sp. Px6-4 catalyzes the decarboxylation reaction of lignin monomers and phenolic compounds such as p-coumaric acid, caffeic acid, and ferulic acid into their corresponding 4-vinyl derivatives, that is, 4-vinylphenol, 4-vinylcatechol, and 4-vinylguaiacol, respectively. Among various ferulic acid decarboxylase enzymes, we chose the FADase from Enterobacter sp. Px6-4, whose crystal structure is known, and produced mutants to enhance its catalytic activity by random and site-directed mutagenesis. After three rounds of sequential mutations, FADase(F95L/D112N/V151I) showed approximately 34-fold higher catalytic activity than wild-type for the production of 4-vinylguaiacol from ferulic acid. Docking analyses suggested that the increased activity of FADase(F95L/D112N/V151I) could be due to formation of compact active site compared with that of the wild-type FADase. Considering the amount of phenolic compounds such as lignin monomers in the biomass components, successfully bioengineered FADase(F95L/D112N/V151I) from Enterobacter sp. Px6-4 could provide an ecofriendly biocatalytic tool for producing diverse styrene derivatives from biomass. PMID:26059194

  10. Characterization of Red/Green Cyanobacteriochrome NpR6012g4 by Solution Nuclear Magnetic Resonance Spectroscopy: A Hydrophobic Pocket for the C15-E,anti Chromophore in the Photoproduct.

    PubMed

    Rockwell, Nathan C; Martin, Shelley S; Lim, Sunghyuk; Lagarias, J Clark; Ames, James B

    2015-06-23

    Cyanobacteriochromes (CBCRs) are cyanobacterial photosensory proteins distantly related to phytochromes. Like phytochromes, CBCRs reversibly photoconvert between a dark-stable state and a photoproduct via photoisomerization of the 15,16-double bond of their linear tetrapyrrole (bilin) chromophores. CBCRs provide cyanobacteria with complete coverage of the visible spectrum and near-ultraviolet region. One CBCR subfamily, the canonical red/green CBCRs typified by AnPixJg2 and NpR6012g4, can function as sensors of light color or intensity because of their great variation in photoproduct stability. The mechanistic basis for detection of green light by the photoproduct state in this subfamily has proven to be a challenging research topic, with competing hydration and trapped-twist models proposed. Here, we use ¹³C-edited and ¹⁵N-edited ¹H-¹H NOESY solution nuclear magnetic resonance spectroscopy to probe changes in chromophore configuration and protein-chromophore interactions in the NpR6012g4 photocycle. Our results confirm a C15-Z,anti configuration for the red-absorbing dark state and reveal a C15-E,anti configuration for the green-absorbing photoproduct. The photoactive chromophore D-ring is located in a hydrophobic environment in the photoproduct, surrounded by both aliphatic and aromatic residues. Characterization of variant proteins demonstrates that no aliphatic residue is essential for photoproduct tuning. Taken together, our results support the trapped-twist model over the hydration model for the red/green photocycle of NpR6012g4. PMID:25989712

  11. 1H, 13C, and 15N chemical shift assignments of cyanobacteriochrome NpR6012g4 in the green-absorbing photoproduct state.

    PubMed

    Lim, Sunghyuk; Yu, Qinhong; Rockwell, Nathan C; Martin, Shelley S; Lagarias, J Clark; Ames, James B

    2016-04-01

    Cyanobacteriochromes (CBCRs) are cyanobacterial photosensory proteins with a tetrapyrrole (bilin) chromophore that belong to the phytochrome superfamily. Like phytochromes, CBCRs photoconvert between two photostates with distinct spectral properties. NpR6012g4 from Nostoc punctiforme is a model system for widespread CBCRs with conserved red/green photocycles. Atomic-level structural information for the photoproduct state in this subfamily is not known. Here, we report NMR backbone chemical shift assignments of the light-activated state of NpR6012g4 (BMRB no. 26577) as a first step toward determining its atomic resolution structure. PMID:26537963

  12. eta-prime photoproduction on the proton for photon energies from 1.527 to 2.227 GeV

    SciTech Connect

    M. Dugger; J. P. Ball; P. Collins; E. Pasyuk; B. G. Ritchie

    2006-01-13

    Differential cross sections for the reaction gamma p {yields} eta-prime p have been measured with the CLAS spectrometer and a tagged photon beam with energies from 1.527 to 2.227 GeV. The results reported here possess much greater accuracy than previous measurements. Analyses of these data indicate for the first time the coupling of the eta prime N channel to both the S{sub 11}(1535) and P{sub 11}(1710) resonances, known to couple strongly to the eta N channel in photoproduction on the proton, and the importance of j=3/2 resonances in the process.

  13. High efficiency antireflection coating in MWIR region (3.6-4.9 μm) simultaneously effective for Germanium and Silicon optics

    NASA Astrophysics Data System (ADS)

    Bhatt, Meenakshi; Nautiyal, B. B.; Bandyopadhyay, P. K.

    2010-01-01

    Antireflection coatings have critical importance in thermal imaging system working in MWIR region (3-5 μm) since optics of high refractive index materials are used. Germanium (Ge) and Silicon (Si) optics are used extensively in the MWIR thermal systems. In this paper a study has been carried out on the design and fabrication of multi-substrate antireflection coating effective for Germanium and Silicon optics in MWIR (3.6-4.9 μm) region. The wave band 3.6-4.9 μm is chosen for the reported work because detector system used in MWIR region has a band selection filter effective in the same wavelength region and atmospheric transmission window in MWIR region is effective in 3-5 μm spectral band. Comprehensive search method was used to design the multilayer stack on the substrate. The coating materials used in the design were Germanium (Ge), Hafnium oxide (HfO 2) and Y-Ba-Fluoride (IR-F625). The fabrication of coating was made in a coating plant fitted with Cryo pump system and residual gas analyzer (RGA). The evaporation was carried out at high vacuum (2-6 × 10 -6 mbar) with the help of electron beam gun system and layer thicknesses were measured with crystal monitor. The result achieved for the antireflection coating was 98.5% average transmission in 3.6-4.9 μm band for Germanium and Silicon optics. This work will be helpful in reducing the plant operation time, material and power consumption, as two different kinds of optics are simultaneously coated in a single coating cycle.

  14. Hydrogen Photoproduction by Nutrient-Deprived Chalamydomonas reinhardtii Cells Immobilized Within Thin Alginate Films Under Aerobic and Anaerobic Conditions

    SciTech Connect

    Kosourov, S. N.; Seibert, M.

    2009-01-01

    A new technique for immobilizing H{sub 2}-photoproducing green algae within a thin (<400 {micro}m) alginate film has been developed. Alginate films with entrapped sulfur/phosphorus-deprived Chlamydomonas reinhardtii, strain cc124, cells demonstrate (a) higher cell density (up to 2,000 {micro}g Chl mL{sup -1} of matrix), (b) kinetics of H{sub 2} photoproduction similar to sulfur-deprived suspension cultures, (c) higher specific rates (up to 12.5 {micro}mol mg{sup -1} Chl h{sup -1}) of H{sub 2} evolution, (d) light conversion efficiencies to H{sub 2} of over 1% and (e) unexpectedly high resistance of the H{sub 2}-photoproducing system to inactivation by atmospheric O{sub 2}. The algal cells, entrapped in alginate and then placed in vials containing 21% O{sub 2} in the headspace, evolved up to 67% of the H{sub 2} gas produced under anaerobic conditions. The results indicate that the lower susceptibility of the immobilized algal H{sub 2}-producing system to inactivation by O{sub 2} depends on two factors: (a) the presence of acetate in the medium, which supports higher rates of respiration and (b) the capability of the alginate polymer itself to effectively separate the entrapped cells from O{sub 2} in the liquid and headspace and restrict O{sub 2} diffusion into the matrix. The strategy presented for immobilizing algal cells within thin polymeric matrices shows the potential for scale-up and possible future applications.

  15. Rapid deamination of cyclobutane pyrimidine dimer photoproducts at TCG sites in a translationally and rotationally positioned nucleosome in vivo.

    PubMed

    Cannistraro, Vincent J; Pondugula, Santhi; Song, Qian; Taylor, John-Stephen

    2015-10-30

    Sunlight-induced C to T mutation hot spots in skin cancers occur primarily at methylated CpG sites that coincide with sites of UV-induced cyclobutane pyrimidine dimer (CPD) formation. The C and 5-methyl-C in CPDs are not stable and deaminate to U and T, respectively, which leads to the insertion of A by the DNA damage bypass polymerase η, thereby defining a probable mechanism for the origin of UV-induced C to T mutations. Deamination rates for T(m)CG CPDs have been found to vary 12-fold with rotational position in a nucleosome in vitro. To determine the influence of nucleosome structure on deamination rates in vivo, we determined the deamination rates of CPDs at TCG sites in a stably positioned nucleosome within the FOS promoter in HeLa cells. A procedure for in vivo hydroxyl radical footprinting with Fe-EDTA was developed, and, together with results from a cytosine methylation protection assay, we determined the translational and rotational positions of the TCG sites. Consistent with the in vitro observations, deamination was slower for one CPD located at an intermediate rotational position compared with two other sites located at outside positions, and all were much faster than for CPDs at non-TCG sites. Photoproduct formation was also highly suppressed at one site, possibly due to its interaction with a histone tail. Thus, it was shown that CPDs of TCG sites deaminate the fastest in vivo and that nucleosomes can modulate both their formation and deamination, which could contribute to the UV mutation hot spots and cold spots. PMID:26354431

  16. Rapid deamination of cyclobutane pyrimidine dimer photoproducts at TCG sites in a translationally and rotationally positioned nucleosome in vivo.

    PubMed

    Cannistraro, Vincent J; Pondugula, Santhi; Song, Qian; Taylor, John-Stephen

    2015-10-30

    Sunlight-induced C to T mutation hot spots in skin cancers occur primarily at methylated CpG sites that coincide with sites of UV-induced cyclobutane pyrimidine dimer (CPD) formation. The C and 5-methyl-C in CPDs are not stable and deaminate to U and T, respectively, which leads to the insertion of A by the DNA damage bypass polymerase η, thereby defining a probable mechanism for the origin of UV-induced C to T mutations. Deamination rates for T(m)CG CPDs have been found to vary 12-fold with rotational position in a nucleosome in vitro. To determine the influence of nucleosome structure on deamination rates in vivo, we determined the deamination rates of CPDs at TCG sites in a stably positioned nucleosome within the FOS promoter in HeLa cells. A procedure for in vivo hydroxyl radical footprinting with Fe-EDTA was developed, and, together with results from a cytosine methylation protection assay, we determined the translational and rotational positions of the TCG sites. Consistent with the in vitro observations, deamination was slower for one CPD located at an intermediate rotational position compared with two other sites located at outside positions, and all were much faster than for CPDs at non-TCG sites. Photoproduct formation was also highly suppressed at one site, possibly due to its interaction with a histone tail. Thus, it was shown that CPDs of TCG sites deaminate the fastest in vivo and that nucleosomes can modulate both their formation and deamination, which could contribute to the UV mutation hot spots and cold spots.

  17. Synthesis of N-(6-(4-(Piperazin-1-yl)phenoxy)pyridin-3-yl)benzenesulfonamide Derivatives for the Treatment of Metabolic Syndrome

    PubMed Central

    Bajare, Swapnil; Anthony, Jessy; Nair, Amrutha; Damre, Anagha; B-Rao, Chandrika; Sivaramakrishnan, H.; Wilankar, Chandan; Marita, Rosalind

    2013-01-01

    Metabolic syndrome is a widely prevalent multifactorial disorder associated with an increased risk of cardiovascular disease and type 2 diabetes mellitus. High plasma levels of insulin and glucose due to insulin resistance are a major component of the metabolic disorder. Thiazolidinediones (TZDs) are potent PPARγ ligand and used as insulin sensitizers in the treatment of type 2 diabetes mellitus. They are potent insulin-sensitizing agents but due to adverse effects like hepatotoxicity, a safer alternative of TZDs is highly demanded. Here we report synthesis of N-(6-(4-(piperazin-1-yl)phenoxy)pyridin-3-yl)benzenesulfonamide derivatives as an alternate remedy for insulin resistance. PMID:25374688

  18. Synthesis of N-(6-(4-(Piperazin-1-yl)phenoxy)pyridin-3-yl)benzenesulfonamide Derivatives for the Treatment of Metabolic Syndrome.

    PubMed

    Deka, Nabajyoti; Bajare, Swapnil; Anthony, Jessy; Nair, Amrutha; Damre, Anagha; Patel, Dharmeshkumar; B-Rao, Chandrika; Sivaramakrishnan, H; Mutt, Shivaprakash Jagalur; Wilankar, Chandan; Marita, Rosalind

    2013-01-01

    Metabolic syndrome is a widely prevalent multifactorial disorder associated with an increased risk of cardiovascular disease and type 2 diabetes mellitus. High plasma levels of insulin and glucose due to insulin resistance are a major component of the metabolic disorder. Thiazolidinediones (TZDs) are potent PPARγ ligand and used as insulin sensitizers in the treatment of type 2 diabetes mellitus. They are potent insulin-sensitizing agents but due to adverse effects like hepatotoxicity, a safer alternative of TZDs is highly demanded. Here we report synthesis of N-(6-(4-(piperazin-1-yl)phenoxy)pyridin-3-yl)benzenesulfonamide derivatives as an alternate remedy for insulin resistance.

  19. Corrosion behaviour of Al86.0Co7.6Ce6.4 glass forming alloy with different microstructures

    NASA Astrophysics Data System (ADS)

    Li, C. L.; Wang, P.; Sun, S. Q.; Voisey, K. T.; McCartney, D. G.

    2016-10-01

    It has been extensively reported that Al-TM-RE amorphous alloy has excellent mechanical properties and corrosion resistance. In this paper, the corrosion behaviour of an Al86.0Co7.6Ce6.4 glass forming alloy with different microstructures is investigated through electrochemical experiments and microscopy. Results show the effect of microstructure. Laser and electron beam surface melting processes produce rapidly solidified microstructures with different extents of passivation compared to the as-cast alloy. An amorphous surface layer produced by these surface treatments had superior corrosion resistance compared with the crystalline alloy. As-cast and laser treated Al86.0Co7.6Ce6.4 suffered localised corrosion in the Al/Al11Ce3 eutectic region whereas the amorphous material exhibited uniform corrosion. Compared with the electrochemical behaviour of AA2024 and Alclad 2024, the fully amorphous layer prepared by combined laser-electron beam treatment exhibited advantages such as the more negative corrosion potential, the higher pitting potential and the uniform corrosion mechanism, which indicates that this material is a potential anode candidate in the protection of AA2024.

  20. [Retarded excision of pyrimidine dimers in human unstimulated lymphocytes].

    PubMed

    Snopov, S A; Roza, L; de Gruijl, F R

    2006-01-01

    Using immuno-labelling of cyclobutane pyrimidine dimers (CPDs) in nuclei of peripheral lymphocytes after their UVC-irradiation and cultivation, we have found that within the first four hours of cultivation the CPD-specific fluorescent signal from cell nuclei increased. Earlier, a similar increase in binding of antibody specific for pyrimidine (6-4) pyrimidone photoproducts to undenatured DNA isolated from UV-irradiated Chinese hamster ovary cells was reported (Mitchell et al., 1986). Our experiments showed that nucleotide excision repair enzyme might induce such of DNA modification in lymphocyte nuclei that increased specific antibody binding to DNA fragments with lesions. We suggest that enzymatic formation of open structures in DNA predominated qualitatively over dual-incision and excision of these fragments, and resulted in the enhanced exposure of the pyrimidine dimers in nuclei to specific antibodies. The results evidence that nucleotid excision repair in unstimualted human lymphocytes being deficient in dual incision and removal of UV-induced DNA lesions appear to be capable of performing chromatin relaxation and pre-incision uncoiling of DNA fragments with lesions.

  1. Potassium bromate but not X-rays cause unexpectedly elevated levels of DNA breakage similar to those induced by ultraviolet light in Cockayne syndrome (CS-B) fibroblasts.

    PubMed

    Mosesso, P; Penna, S; Pepe, G; Lorenti-Garcia, C; Palitti, F

    2004-01-01

    It has been previously reported that the elevated accumulation of repair incision intermediates in cells from patients with combined characteristics of xeroderma pigmentosum complementation group D (XP-D) and Cockayne syndrome (CS) XP-D/CS fibroblasts following UV irradiation is caused by an "uncontrolled" incision of undamaged genomic DNA induced by UV-DNA-lesions which apparently are not removed. This could be an explanation for the extreme sensitivity of these cells to UV light. In the present study, we confirm the immediate DNA breakage following UV irradiation also for CS group B (CS-B) fibroblasts by DNA migration in the "comet assay" and extend these findings to other lesions such as 8-oxodeoxyguanosine (8-oxodG), selectively induced by KBrO3 treatment. In contrast, X-ray exposure does not induce differential DNA breakage. This indicates that additional lesions other than the UV-induced photoproducts (cyclobutane pyrimidine dimers, CPD, and 6-pyrimidine-4-pyrimidone products, 6-4 PP), such as 8-oxodG, specifically induced by KBrO3, are likely to trigger "uncontrolled" DNA breakage in the undamaged genomic DNA in the CS-B fibroblasts, thus accounting for some of the clinical features of these patients. PMID:15162034

  2. Analysis of a photosensitive lesion induced by sunlamp UV greater than 315 nm exposure of 254-nm-irradiated human cells.

    PubMed

    Rosenstein, B S

    1988-11-01

    Normal human skin fibroblasts were exposed to 0-10 J m-2 of 254 nm UV, incubated 0-16 h and then treated with 0-150 kJ m-2 of sunlamp UV greater than 315 nm. For each treatment, the cells were subjected to alkaline elution in order to measure the yield of single strand breaks (ssb) produced. It was found that treatment of 254-nm-irradiated cells with sunlamp UV greater than 315 nm resulted in the production of a higher level of ssb than that produced by separate exposures. Hence, lesions are produced by the 254 nm irradiation that are photolyzed through exposure to sunlamp UV greater than 315 nm. Approximately 50% of these lesions are removed following a 2-4 h incubation of the 254-nm-irradiated cells and nearly complete removal is achieved by 16 h. In addition, the profiles for elutions performed at pH 12.8 with cells exposed to the combined treatment were indicative of the presence of alkali labile sites. The repair kinetics of this lesion and alkaline lability of the photolysis product suggest that this photosensitive lesion may represent pyrimidine(6-4)pyrimidone photoproducts. Hence, this approach may represent a relatively simple and sensitive assay for the measurement of this DNA damage.

  3. Photoproduction of J/ψ and of high mass e+e− in ultra-peripheral Au +Au collisions at root sNN = 200GeV

    SciTech Connect

    Afanasiev, S.; PHENIX Collaboration

    2009-09-01

    We present the first measurement of photoproduction of J/{Psi} and of two-photon production of high-mass e{sup +}e{sup -} pairs in electromagnetic (or ultra-peripheral) nucleus-nucleus interactions, using Au + Au data at {radical}s{sub NN}. The events are tagged with forward neutrons emitted following Coulomb excitation of one or both Au* nuclei. The event sample consists of 28 events with me{sup +}e{sup -} > 2 GeV/c{sup 2} with zero like-sign background. The measured cross sections at midrapidity of d{sigma}/dy (J/{Psi} + Xn, y = 0) = 76 {+-} 33(stat) {+-} 11(syst) {mu}b and d{sup 2}{sigma}/dm dy (e{sup +}e{sup -} + Xn, y = 0) = 86 {+-} 23(stat) {+-} 16(syst) {mu}b/(GeV/c{sup 2}) for me{sup +}e{sup -} {element_of} [2.0,2.8] GeV/c{sup 2} have been compared and found to be consistent with models for photoproduction of J/{Psi} and QED based calculations of two-photon production of e{sup +}e{sup -} pairs.

  4. Photoproduction of J/ψ and of high mass e+e− in ultra-peripheral Au +Au collisions at root sNN = 200 GeV

    SciTech Connect

    Afanasiev, S.

    2009-08-01

    We present the first measurement of photoproduction of J/{Psi} and of two-photon production of high-mass e{sup +}e{sup -} pairs in electromagnetic (or ultra-peripheral) nucleus-nucleus interactions, using Au + Au data at {radical}s{sub NN}. The events are tagged with forward neutrons emitted following Coulomb excitation of one or both Au* nuclei. The event sample consists of 28 events with me{sup +}e{sup -} > 2 GeV/c{sup 2} with zero like-sign background. The measured cross sections at midrapidity of d{sigma}/dy (J/{Psi} + Xn, y = 0) = 76 {+-} 33(stat) {+-} 11(syst) {mu}b and d{sup 2}{sigma}/dm dy (e{sup +}e{sup -} + Xn, y = 0) = 86 {+-} 23(stat) {+-} 16(syst) {mu}b/(GeV/c{sup 2}) for me{sup +}e{sup -} {element_of} [2.0,2.8] GeV/c{sup 2} have been compared and found to be consistent with models for photoproduction of J/{Psi} and QED based calculations of two-photon production of e{sup +}e{sup -} pairs.

  5. Dephenolization of stored olive-mill wastewater, using four different adsorbing matrices to attain a low-cost feedstock for hydrogen photo-production.

    PubMed

    Padovani, Giulia; Pintucci, Cristina; Carlozzi, Pietro

    2013-06-01

    This investigation deals with the conversion of olive-mill wastewater (OMW) into several feedstocks suitable for hydrogen photo-production. The goal was reached by means of two sequential steps: (i) a pre-treatment process of stored-OMW for the removal of polyphenols, which made it possible to obtain several effluents, and (ii) a photo-fermentative process for hydrogen production by means of Rhodopseudomonas palustris sp. Four different adsorbent matrices (Azolla, granular active carbon, resin, and zeolite) were used to dephenolize stored-OMW. The four liquid fractions attained by using the above process created the same number of effluents, and these were diluted with water and then used for hydrogen photo-production. The maximum hydrogen production rate (14.31 mL/L/h) was attained with the photo-fermenter containing 25% of the effluent, which came from the pre-treatment of stored-OMW using granular active carbon. Using the carbon effluent as feedstock, the greatest light conversion efficiency of 2.29% was achieved. PMID:23612177

  6. Observation of pi+pi-pi+pi- photoproduction in ultraperipheral heavy-ion collisions at sqrt sNN = 200 GeV at the STAR detector

    SciTech Connect

    STAR Collaboration; Abelev, Betty

    2010-07-05

    We present a measurement of {pi}{sup +}{pi}{sup -}{pi}{sup +}{pi}{sup -} photonuclear production in ultra-peripheral Au-Au collisions at {radical}s{sub NN} = 200 GeV from the STAR experiment. The {pi}{sup +}{pi}{sup -}{pi}{sup +}{pi}{sup -} final states are observed at low transverse momentum and are accompanied by mutual nuclear excitation of the beam particles. The strong enhancement of the production cross section at low transverse momentum is consistent with coherent photoproduction. The {pi}{sup +}{pi}{sup -}{pi}{sup +}{pi}{sup -} invariant mass spectrum of the coherent events exhibits a broad peak around 1540 {+-} 40 MeV/c{sup 2} with a width of 570 {+-} 60 MeV/c{sup 2}, in agreement with the photoproduction data for the {rho}{sup 0}(1700). We do not observe a corresponding peak in the {pi}{sup +}{pi}{sup -} final state and measure an upper limit for the ratio of the branching fractions of the {rho}{sup 0}(1700) to {pi}{sup +}{pi}{sup -} and {pi}{sup +}{pi}{sup -}{pi}{sup +}{pi}{sup -} of 2.5% at 90% confidence level. The ratio of {rho}{sup 0}(1700) and {rho}{sup 0}(770) coherent production cross sections is measured to be 13.4 {+-} 0.8{sub stat.} {+-} 4.4{sub syst.}%.

  7. Exclusive J /ψ Photoproduction off Protons in Ultraperipheral p -Pb Collisions at √{sNN }=5.02 TeV

    NASA Astrophysics Data System (ADS)

    Abelev, B.; Adam, J.; Adamová, D.; Aggarwal, M. M.; Aglieri Rinella, G.; Agnello, M.; Agostinelli, A.; Agrawal, N.; Ahammed, Z.; Ahmad, N.; Ahmed, I.; Ahn, S. U.; Ahn, S. A.; Aimo, I.; Aiola, S.; Ajaz, M.; Akindinov, A.; Alam, S. N.; Aleksandrov, D.; Alessandro, B.; Alexandre, D.; Alici, A.; Alkin, A.; Alme, J.; Alt, T.; Altinpinar, S.; Altsybeev, I.; Alves Garcia Prado, C.; Andrei, C.; Andronic, A.; Anguelov, V.; Anielski, J.; Antičić, T.; Antinori, F.; Antonioli, P.; Aphecetche, L.; Appelshäuser, H.; Arcelli, S.; Armesto, N.; Arnaldi, R.; Aronsson, T.; Arsene, I. C.; Arslandok, M.; Augustinus, A.; Averbeck, R.; Awes, T. C.; Azmi, M. D.; Bach, M.; Badalà, A.; Baek, Y. W.; Bagnasco, S.; Bailhache, R.; Bala, R.; Baldisseri, A.; Baltasar Dos Santos Pedrosa, F.; Baral, R. C.; Barbera, R.; Barile, F.; Barnaföldi, G. G.; Barnby, L. S.; Barret, V.; Bartke, J.; Basile, M.; Bastid, N.; Basu, S.; Bathen, B.; Batigne, G.; Batista Camejo, A.; Batyunya, B.; Batzing, P. C.; Baumann, C.; Bearden, I. G.; Beck, H.; Bedda, C.; Behera, N. K.; Belikov, I.; Bellini, F.; Bellwied, R.; Belmont-Moreno, E.; Belmont, R.; Belyaev, V.; Bencedi, G.; Beole, S.; Berceanu, I.; Bercuci, A.; Berdnikov, Y.; Berenyi, D.; Berger, M. E.; Bertens, R. A.; Berzano, D.; Betev, L.; Bhasin, A.; Bhat, I. R.; Bhati, A. K.; Bhattacharjee, B.; Bhom, J.; Bianchi, L.; Bianchi, N.; Bianchin, C.; Bielčík, J.; Bielčíková, J.; Bilandzic, A.; Bjelogrlic, S.; Blanco, F.; Blau, D.; Blume, C.; Bock, F.; Bogdanov, A.; Bøggild, H.; Bogolyubsky, M.; Böhmer, F. V.; Boldizsár, L.; Bombara, M.; Book, J.; Borel, H.; Borissov, A.; Bossú, F.; Botje, M.; Botta, E.; Böttger, S.; Braun-Munzinger, P.; Bregant, M.; Breitner, T.; Broker, T. A.; Browning, T. A.; Broz, M.; Bruna, E.; Bruno, G. E.; Budnikov, D.; Buesching, H.; Bufalino, S.; Buncic, P.; Busch, O.; Buthelezi, Z.; Caffarri, D.; Cai, X.; Caines, H.; Calero Diaz, L.; Caliva, A.; Calvo Villar, E.; Camerini, P.; Carena, F.; Carena, W.; Castillo Castellanos, J.; Casula, E. A. R.; Catanescu, V.; Cavicchioli, C.; Ceballos Sanchez, C.; Cepila, J.; Cerello, P.; Chang, B.; Chapeland, S.; Charvet, J. L.; Chattopadhyay, S.; Chattopadhyay, S.; Chelnokov, V.; Cherney, M.; Cheshkov, C.; Cheynis, B.; Chibante Barroso, V.; Chinellato, D. D.; Chochula, P.; Chojnacki, M.; Choudhury, S.; Christakoglou, P.; Christensen, C. H.; Christiansen, P.; Chujo, T.; Chung, S. U.; Cicalo, C.; Cifarelli, L.; Cindolo, F.; Cleymans, J.; Colamaria, F.; Colella, D.; Collu, A.; Colocci, M.; Conesa Balbastre, G.; Conesa Del Valle, Z.; Connors, M. E.; Contreras, J. G.; Cormier, T. M.; Corrales Morales, Y.; Cortese, P.; Cortés Maldonado, I.; Cosentino, M. R.; Costa, F.; Crochet, P.; Cruz Albino, R.; Cuautle, E.; Cunqueiro, L.; Dainese, A.; Dang, R.; Danu, A.; Das, D.; Das, I.; Das, K.; Das, S.; Dash, A.; Dash, S.; de, S.; Delagrange, H.; Deloff, A.; Dénes, E.; D'Erasmo, G.; de Caro, A.; de Cataldo, G.; de Cuveland, J.; de Falco, A.; de Gruttola, D.; De Marco, N.; de Pasquale, S.; de Rooij, R.; Diaz Corchero, M. A.; Dietel, T.; Dillenseger, P.; Divià, R.; di Bari, D.; di Liberto, S.; di Mauro, A.; di Nezza, P.; Djuvsland, Ø.; Dobrin, A.; Dobrowolski, T.; Domenicis Gimenez, D.; Dönigus, B.; Dordic, O.; Dørheim, S.; Dubey, A. K.; Dubla, A.; Ducroux, L.; Dupieux, P.; Dutta Majumdar, A. K.; Hilden, T. E.; Ehlers, R. J.; Elia, D.; Engel, H.; Erazmus, B.; Erdal, H. A.; Eschweiler, D.; Espagnon, B.; Esposito, M.; Estienne, M.; Esumi, S.; Evans, D.; Evdokimov, S.; Fabris, D.; Faivre, J.; Falchieri, D.; Fantoni, A.; Fasel, M.; Fehlker, D.; Feldkamp, L.; Felea, D.; Feliciello, A.; Feofilov, G.; Ferencei, J.; Fernández Téllez, A.; Ferreiro, E. G.; Ferretti, A.; Festanti, A.; Figiel, J.; Figueredo, M. A. S.; Filchagin, S.; Finogeev, D.; Fionda, F. M.; Fiore, E. M.; Floratos, E.; Floris, M.; Foertsch, S.; Foka, P.; Fokin, S.; Fragiacomo, E.; Francescon, A.; Frankenfeld, U.; Fuchs, U.; Furget, C.; Furs, A.; Fusco Girard, M.; Gaardhøje, J. J.; Gagliardi, M.; Gago, A. M.; Gallio, M.; Gangadharan, D. R.; Ganoti, P.; Gao, C.; Garabatos, C.; Garcia-Solis, E.; Gargiulo, C.; Garishvili, I.; Gerhard, J.; Germain, M.; Gheata, A.; Gheata, M.; Ghidini, B.; Ghosh, P.; Ghosh, S. K.; Gianotti, P.; Giubellino, P.; Gladysz-Dziadus, E.; Glässel, P.; Gomez Ramirez, A.; González-Zamora, P.; Gorbunov, S.; Görlich, L.; Gotovac, S.; Graczykowski, L. K.; Grelli, A.; Grigoras, A.; Grigoras, C.; Grigoriev, V.; Grigoryan, A.; Grigoryan, S.; Grinyov, B.; Grion, N.; Grosse-Oetringhaus, J. F.; Grossiord, J.-Y.; Grosso, R.; Guber, F.; Guernane, R.; Guerzoni, B.; Guilbaud, M.; Gulbrandsen, K.; Gulkanyan, H.; Gumbo, M.; Gunji, T.; Gupta, A.; Gupta, R.; Khan, K. H.; Haake, R.; Haaland, Ø.; Hadjidakis, C.; Haiduc, M.; Hamagaki, H.; Hamar, G.; Hanratty, L. D.; Hansen, A.; Harris, J. W.; Hartmann, H.; Harton, A.; Hatzifotiadou, D.; Hayashi, S.; Heckel, S. T.; Heide, M.; Helstrup, H.; Herghelegiu, A.; Herrera Corral, G.; Hess, B. A.; Hetland, K. F.; Hippolyte, B.; Hladky, J.; Hristov, P.; Huang, M.; Humanic, T. J.; Hussain, N.; Hutter, D.; Hwang, D. S.; Ilkaev, R.; Ilkiv, I.; Inaba, M.; Innocenti, G. M.; Ionita, C.; Ippolitov, M.; Irfan, M.; Ivanov, M.; Ivanov, V.; Jachołkowski, A.; Jacobs, P. M.; Jahnke, C.; Jang, H. J.; Janik, M. A.; Jayarathna, P. H. S. Y.; Jena, C.; Jena, S.; Jimenez Bustamante, R. T.; Jones, P. G.; Jung, H.; Jusko, A.; Kadyshevskiy, V.; Kalcher, S.; Kalinak, P.; Kalweit, A.; Kamin, J.; Kang, J. H.; Kaplin, V.; Kar, S.; Karasu Uysal, A.; Karavichev, O.; Karavicheva, T.; Karpechev, E.; Kebschull, U.; Keidel, R.; Keijdener, D. L. D.; Keil Svn, M.; Khan, M. M.; Khan, P.; Khan, S. A.; Khanzadeev, A.; Kharlov, Y.; Kileng, B.; Kim, B.; Kim, D. W.; Kim, D. J.; Kim, J. S.; Kim, M.; Kim, M.; Kim, S.; Kim, T.; Kirsch, S.; Kisel, I.; Kiselev, S.; Kisiel, A.; Kiss, G.; Klay, J. L.; Klein, J.; Klein-Bösing, C.; Kluge, A.; Knichel, M. L.; Knospe, A. G.; Kobdaj, C.; Kofarago, M.; Köhler, M. K.; Kollegger, T.; Kolojvari, A.; Kondratiev, V.; Kondratyeva, N.; Konevskikh, A.; Kovalenko, V.; Kowalski, M.; Kox, S.; Koyithatta Meethaleveedu, G.; Kral, J.; Králik, I.; Kravčáková, A.; Krelina, M.; Kretz, M.; Krivda, M.; Krizek, F.; Kryshen, E.; Krzewicki, M.; Kučera, V.; Kucheriaev, Y.; Kugathasan, T.; Kuhn, C.; Kuijer, P. G.; Kulakov, I.; Kumar, J.; Kurashvili, P.; Kurepin, A.; Kurepin, A. B.; Kuryakin, A.; Kushpil, S.; Kweon, M. J.; Kwon, Y.; Ladron de Guevara, P.; Lagana Fernandes, C.; Lakomov, I.; Langoy, R.; Lara, C.; Lardeux, A.; Lattuca, A.; La Pointe, S. L.; La Rocca, P.; Lea, R.; Leardini, L.; Lee, G. R.; Legrand, I.; Lehnert, J.; Lemmon, R. C.; Lenti, V.; Leogrande, E.; Leoncino, M.; León Monzón, I.; Lévai, P.; Li, S.; Lien, J.; Lietava, R.; Lindal, S.; Lindenstruth, V.; Lippmann, C.; Lisa, M. A.; Ljunggren, H. M.; Lodato, D. F.; Loenne, P. I.; Loggins, V. R.; Loginov, V.; Lohner, D.; Loizides, C.; Lopez, X.; López Torres, E.; Lu, X.-G.; Luettig, P.; Lunardon, M.; Luparello, G.; Ma, R.; Maevskaya, A.; Mager, M.; Mahapatra, D. P.; Mahmood, S. M.; Maire, A.; Majka, R. D.; Malaev, M.; Maldonado Cervantes, I.; Malinina, L.; Mal'Kevich, D.; Malzacher, P.; Mamonov, A.; Manceau, L.; Manko, V.; Manso, F.; Manzari, V.; Marchisone, M.; Mareš, J.; Margagliotti, G. V.; Margotti, A.; Marín, A.; Markert, C.; Marquard, M.; Martashvili, I.; Martin, N. A.; Martinengo, P.; Martínez, M. I.; Martínez García, G.; Martin Blanco, J.; Martynov, Y.; Mas, A.; Masciocchi, S.; Masera, M.; Masoni, A.; Massacrier, L.; Mastroserio, A.; Matyja, A.; Mayer, C.; Mazer, J.; Mazzoni, M. A.; Meddi, F.; Menchaca-Rocha, A.; Meninno, E.; Mercado Pérez, J.; Meres, M.; Miake, Y.; Mikhaylov, K.; Milano, L.; Milosevic, J.; Mischke, A.; Mishra, A. N.; Miśkowiec, D.; Mitra, J.; Mitu, C. M.; Mlynarz, J.; Mohammadi, N.; Mohanty, B.; Molnar, L.; Montaño Zetina, L.; Montes, E.; Morando, M.; Moreira de Godoy, D. A.; Moretto, S.; Morreale, A.; Morsch, A.; Muccifora, V.; Mudnic, E.; Mühlheim, D.; Muhuri, S.; Mukherjee, M.; Müller, H.; Munhoz, M. G.; Murray, S.; Musa, L.; Musinsky, J.; Nandi, B. K.; Nania, R.; Nappi, E.; Nattrass, C.; Nayak, K.; Nayak, T. K.; Nazarenko, S.; Nedosekin, A.; Nicassio, M.; Niculescu, M.; Nielsen, B. S.; Nikolaev, S.; Nikulin, S.; Nikulin, V.; Nilsen, B. S.; Noferini, F.; Nomokonov, P.; Nooren, G.; Norman, J.; Nyanin, A.; Nystrand, J.; Oeschler, H.; Oh, S.; Oh, S. K.; Okatan, A.; Olah, L.; Oleniacz, J.; Oliveira da Silva, A. C.; Onderwaater, J.; Oppedisano, C.; Ortiz Velasquez, A.; Oskarsson, A.; Otwinowski, J.; Oyama, K.; Ozdemir, M.; Sahoo, P.; Pachmayer, Y.; Pachr, M.; Pagano, P.; Paić, G.; Painke, F.; Pajares, C.; Pal, S. K.; Palmeri, A.; Pant, D.; Papikyan, V.; Pappalardo, G. S.; Pareek, P.; Park, W. J.; Parmar, S.; Passfeld, A.; Patalakha, D. I.; Paticchio, V.; Paul, B.; Pawlak, T.; Peitzmann, T.; Pereira da Costa, H.; Pereira de Oliveira Filho, E.; Peresunko, D.; Pérez Lara, C. E.; Pesci, A.; Peskov, V.; Pestov, Y.; Petráček, V.; Petran, M.; Petris, M.; Petrovici, M.; Petta, C.; Piano, S.; Pikna, M.; Pillot, P.; Pinazza, O.; Pinsky, L.; Piyarathna, D. B.; Płoskoń, M.; Planinic, M.; Pluta, J.; Pochybova, S.; Podesta-Lerma, P. L. M.; Poghosyan, M. G.; Pohjoisaho, E. H. O.; Polichtchouk, B.; Poljak, N.; Pop, A.; Porteboeuf-Houssais, S.; Porter, J.; Potukuchi, B.; Prasad, S. K.; Preghenella, R.; Prino, F.; Pruneau, C. A.; Pshenichnov, I.; Puddu, G.; Pujahari, P.; Punin, V.; Putschke, J.; Qvigstad, H.; Rachevski, A.; Raha, S.; Rak, J.; Rakotozafindrabe, A.; Ramello, L.; Raniwala, R.; Raniwala, S.; Räsänen, S. S.; Rascanu, B. T.; Rathee, D.; Rauf, A. W.; Razazi, V.; Read, K. F.; Real, J. S.; Redlich, K.; Reed, R. J.; Rehman, A.; Reichelt, P.; Reicher, M.; Reidt, F.; Renfordt, R.; Reolon, A. R.; Reshetin, A.; Rettig, F.; Revol, J.-P.; Reygers, K.; Riabov, V.; Ricci, R. A.; Richert, T.; Richter, M.; Riedler, P.; Riegler, W.; Riggi, F.; Rivetti, A.; Rocco, E.; Rodríguez Cahuantzi, M.; Rodriguez Manso, A.; Røed, K.; Rogochaya, E.; Rohni, S.; Rohr, D.; Röhrich, D.; Romita, R.; Ronchetti, F.; Ronflette, L.; Rosnet, P.; Rossi, A.; Roukoutakis, F.; Roy, A.; Roy, C.; Roy, P.; Rubio Montero, A. J.; Rui, R.; Russo, R.; Ryabinkin, E.; Ryabov, Y.; Rybicki, A.; Sadovsky, S.; Šafařík, K.; Sahlmuller, B.; Sahoo, R.; Sahu, P. K.; Saini, J.; Sakai, S.; Salgado, C. A.; Salzwedel, J.; Sambyal, S.; Samsonov, V.; Sanchez Castro, X.; Sánchez Rodríguez, F. J.; Šándor, L.; Sandoval, A.; Sano, M.; Santagati, G.; Sarkar, D.; Scapparone, E.; Scarlassara, F.; Scharenberg, R. P.; Schiaua, C.; Schicker, R.; Schmidt, C.; Schmidt, H. R.; Schuchmann, S.; Schukraft, J.; Schulc, M.; Schuster, T.; Schutz, Y.; Schwarz, K.; Schweda, K.; Scioli, G.; Scomparin, E.; Scott, R.; Segato, G.; Seger, J. E.; Sekiguchi, Y.; Selyuzhenkov, I.; Seo, J.; Serradilla, E.; Sevcenco, A.; Shabetai, A.; Shabratova, G.; Shahoyan, R.; Shangaraev, A.; Sharma, N.; Sharma, S.; Shigaki, K.; Shtejer, K.; Sibiriak, Y.; Siddhanta, S.; Siemiarczuk, T.; Silvermyr, D.; Silvestre, C.; Simatovic, G.; Singaraju, R.; Singh, R.; Singha, S.; Singhal, V.; Sinha, B. C.; Sinha, T.; Sitar, B.; Sitta, M.; Skaali, T. B.; Skjerdal, K.; Slupecki, M.; Smirnov, N.; Snellings, R. J. M.; Søgaard, C.; Soltz, R.; Song, J.; Song, M.; Soramel, F.; Sorensen, S.; Spacek, M.; Spiriti, E.; Sputowska, I.; Spyropoulou-Stassinaki, M.; Srivastava, B. K.; Stachel, J.; Stan, I.; Stefanek, G.; Steinpreis, M.; Stenlund, E.; Steyn, G.; Stiller, J. H.; Stocco, D.; Stolpovskiy, M.; Strmen, P.; Suaide, A. A. P.; Sugitate, T.; Suire, C.; Suleymanov, M.; Sultanov, R.; Šumbera, M.; Susa, T.; Symons, T. J. M.; Szabo, A.; Szanto de Toledo, A.; Szarka, I.; Szczepankiewicz, A.; Szymanski, M.; Takahashi, J.; Tangaro, M. A.; Tapia Takaki, J. D.; Tarantola Peloni, A.; Tarazona Martinez, A.; Tarzila, M. G.; Tauro, A.; Tejeda Muñoz, G.; Telesca, A.; Terrevoli, C.; Thäder, J.; Thomas, D.; Tieulent, R.; Timmins, A. R.; Toia, A.; Trubnikov, V.; Trzaska, W. H.; Tsuji, T.; Tumkin, A.; Turrisi, R.; Tveter, T. S.; Ullaland, K.; Uras, A.; Usai, G. L.; Vajzer, M.; Vala, M.; Valencia Palomo, L.; Vallero, S.; Vande Vyvre, P.; van der Maarel, J.; van Hoorne, J. W.; van Leeuwen, M.; Vargas, A.; Vargyas, M.; Varma, R.; Vasileiou, M.; Vasiliev, A.; Vechernin, V.; Veldhoen, M.; Velure, A.; Venaruzzo, M.; Vercellin, E.; Vergara Limón, S.; Vernet, R.; Verweij, M.; Vickovic, L.; Viesti, G.; Viinikainen, J.; Vilakazi, Z.; Villalobos Baillie, O.; Vinogradov, A.; Vinogradov, L.; Vinogradov, Y.; Virgili, T.; Viyogi, Y. P.; Vodopyanov, A.; Völkl, M. A.; Voloshin, K.; Voloshin, S. A.; Volpe, G.; von Haller, B.; Vorobyev, I.; Vranic, D.; Vrláková, J.; Vulpescu, B.; Vyushin, A.; Wagner, B.; Wagner, J.; Wagner, V.; Wang, M.; Wang, Y.; Watanabe, D.; Weber, M.; Wessels, J. P.; Westerhoff, U.; Wiechula, J.; Wikne, J.; Wilde, M.; Wilk, G.; Wilkinson, J.; Williams, M. C. S.; Windelband, B.; Winn, M.; Yaldo, C. G.; Yamaguchi, Y.; Yang, H.; Yang, P.; Yang, S.; Yano, S.; Yasnopolskiy, S.; Yi, J.; Yin, Z.; Yoo, I.-K.; Yushmanov, I.; Zaccolo, V.; Zach, C.; Zaman, A.; Zampolli, C.; Zaporozhets, S.; Zarochentsev, A.; Závada, P.; Zaviyalov, N.; Zbroszczyk, H.; Zgura, I. S.; Zhalov, M.; Zhang, H.; Zhang, X.; Zhang, Y.; Zhao, C.; Zhigareva, N.; Zhou, D.; Zhou, F.; Zhou, Y.; Zhou, Zhuo; Zhu, H.; Zhu, J.; Zhu, X.; Zichichi, A.; Zimmermann, A.; Zimmermann, M. B.; Zinovjev, G.; Zoccarato, Y.; Zyzak, M.; Alice Collaboration

    2014-12-01

    We present the first measurement at the LHC of exclusive J /ψ photoproduction off protons, in ultraperipheral proton-lead collisions at √{sNN }=5.02 TeV . Events are selected with a dimuon pair produced either in the rapidity interval, in the laboratory frame, 2.5 photoproduction cross section in γ p energies from about 20 to 700 GeV, or equivalently, from Bjorken x scaling variable between ˜2 ×10-2 and ˜2 ×10-5, thus indicating no significant change in the gluon density behavior of the proton between HERA and LHC energies.

  8. Exclusive J/ψ photoproduction off protons in ultraperipheral p-Pb collisions at √(s(NN))=5.02 TeV.

    PubMed

    Abelev, B; Adam, J; Adamová, D; Aggarwal, M M; Aglieri Rinella, G; Agnello, M; Agostinelli, A; Agrawal, N; Ahammed, Z; Ahmad, N; Ahmed, I; Ahn, S U; Ahn, S A; Aimo, I; Aiola, S; Ajaz, M; Akindinov, A; Alam, S N; Aleksandrov, D; Alessandro, B; Alexandre, D; Alici, A; Alkin, A; Alme, J; Alt, T; Altinpinar, S; Altsybeev, I; Alves Garcia Prado, C; Andrei, C; Andronic, A; Anguelov, V; Anielski, J; Antičić, T; Antinori, F; Antonioli, P; Aphecetche, L; Appelshäuser, H; Arcelli, S; Armesto, N; Arnaldi, R; Aronsson, T; Arsene, I C; Arslandok, M; Augustinus, A; Averbeck, R; Awes, T C; Azmi, M D; Bach, M; Badalà, A; Baek, Y W; Bagnasco, S; Bailhache, R; Bala, R; Baldisseri, A; Baltasar Dos Santos Pedrosa, F; Baral, R C; Barbera, R; Barile, F; Barnaföldi, G G; Barnby, L S; Barret, V; Bartke, J; Basile, M; Bastid, N; Basu, S; Bathen, B; Batigne, G; Batista Camejo, A; Batyunya, B; Batzing, P C; Baumann, C; Bearden, I G; Beck, H; Bedda, C; Behera, N K; Belikov, I; Bellini, F; Bellwied, R; Belmont-Moreno, E; Belmont, R; Belyaev, V; Bencedi, G; Beole, S; Berceanu, I; Bercuci, A; Berdnikov, Y; Berenyi, D; Berger, M E; Bertens, R A; Berzano, D; Betev, L; Bhasin, A; Bhat, I R; Bhati, A K; Bhattacharjee, B; Bhom, J; Bianchi, L; Bianchi, N; Bianchin, C; Bielčík, J; Bielčíková, J; Bilandzic, A; Bjelogrlic, S; Blanco, F; Blau, D; Blume, C; Bock, F; Bogdanov, A; Bøggild, H; Bogolyubsky, M; Böhmer, F V; Boldizsár, L; Bombara, M; Book, J; Borel, H; Borissov, A; Bossú, F; Botje, M; Botta, E; Böttger, S; Braun-Munzinger, P; Bregant, M; Breitner, T; Broker, T A; Browning, T A; Broz, M; Bruna, E; Bruno, G E; Budnikov, D; Buesching, H; Bufalino, S; Buncic, P; Busch, O; Buthelezi, Z; Caffarri, D; Cai, X; Caines, H; Calero Diaz, L; Caliva, A; Calvo Villar, E; Camerini, P; Carena, F; Carena, W; Castillo Castellanos, J; Casula, E A R; Catanescu, V; Cavicchioli, C; Ceballos Sanchez, C; Cepila, J; Cerello, P; Chang, B; Chapeland, S; Charvet, J L; Chattopadhyay, S; Chattopadhyay, S; Chelnokov, V; Cherney, M; Cheshkov, C; Cheynis, B; Chibante Barroso, V; Chinellato, D D; Chochula, P; Chojnacki, M; Choudhury, S; Christakoglou, P; Christensen, C H; Christiansen, P; Chujo, T; Chung, S U; Cicalo, C; Cifarelli, L; Cindolo, F; Cleymans, J; Colamaria, F; Colella, D; Collu, A; Colocci, M; Conesa Balbastre, G; Conesa Del Valle, Z; Connors, M E; Contreras, J G; Cormier, T M; Corrales Morales, Y; Cortese, P; Cortés Maldonado, I; Cosentino, M R; Costa, F; Crochet, P; Cruz Albino, R; Cuautle, E; Cunqueiro, L; Dainese, A; Dang, R; Danu, A; Das, D; Das, I; Das, K; Das, S; Dash, A; Dash, S; De, S; Delagrange, H; Deloff, A; Dénes, E; D'Erasmo, G; De Caro, A; de Cataldo, G; de Cuveland, J; De Falco, A; De Gruttola, D; De Marco, N; De Pasquale, S; de Rooij, R; Diaz Corchero, M A; Dietel, T; Dillenseger, P; Divià, R; Di Bari, D; Di Liberto, S; Di Mauro, A; Di Nezza, P; Djuvsland, Ø; Dobrin, A; Dobrowolski, T; Domenicis Gimenez, D; Dönigus, B; Dordic, O; Dørheim, S; Dubey, A K; Dubla, A; Ducroux, L; Dupieux, P; Dutta Majumdar, A K; Hilden, T E; Ehlers, R J; Elia, D; Engel, H; Erazmus, B; Erdal, H A; Eschweiler, D; Espagnon, B; Esposito, M; Estienne, M; Esumi, S; Evans, D; Evdokimov, S; Fabris, D; Faivre, J; Falchieri, D; Fantoni, A; Fasel, M; Fehlker, D; Feldkamp, L; Felea, D; Feliciello, A; Feofilov, G; Ferencei, J; Fernández Téllez, A; Ferreiro, E G; Ferretti, A; Festanti, A; Figiel, J; Figueredo, M A S; Filchagin, S; Finogeev, D; Fionda, F M; Fiore, E M; Floratos, E; Floris, M; Foertsch, S; Foka, P; Fokin, S; Fragiacomo, E; Francescon, A; Frankenfeld, U; Fuchs, U; Furget, C; Furs, A; Fusco Girard, M; Gaardhøje, J J; Gagliardi, M; Gago, A M; Gallio, M; Gangadharan, D R; Ganoti, P; Gao, C; Garabatos, C; Garcia-Solis, E; Gargiulo, C; Garishvili, I; Gerhard, J; Germain, M; Gheata, A; Gheata, M; Ghidini, B; Ghosh, P; Ghosh, S K; Gianotti, P; Giubellino, P; Gladysz-Dziadus, E; Glässel, P; Gomez Ramirez, A; González-Zamora, P; Gorbunov, S; Görlich, L; Gotovac, S; Graczykowski, L K; Grelli, A; Grigoras, A; Grigoras, C; Grigoriev, V; Grigoryan, A; Grigoryan, S; Grinyov, B; Grion, N; Grosse-Oetringhaus, J F; Grossiord, J-Y; Grosso, R; Guber, F; Guernane, R; Guerzoni, B; Guilbaud, M; Gulbrandsen, K; Gulkanyan, H; Gumbo, M; Gunji, T; Gupta, A; Gupta, R; Khan, K H; Haake, R; Haaland, Ø; Hadjidakis, C; Haiduc, M; Hamagaki, H; Hamar, G; Hanratty, L D; Hansen, A; Harris, J W; Hartmann, H; Harton, A; Hatzifotiadou, D; Hayashi, S; Heckel, S T; Heide, M; Helstrup, H; Herghelegiu, A; Herrera Corral, G; Hess, B A; Hetland, K F; Hippolyte, B; Hladky, J; Hristov, P; Huang, M; Humanic, T J; Hussain, N; Hutter, D; Hwang, D S; Ilkaev, R; Ilkiv, I; Inaba, M; Innocenti, G M; Ionita, C; Ippolitov, M; Irfan, M; Ivanov, M; Ivanov, V; Jachołkowski, A; Jacobs, P M; Jahnke, C; Jang, H J; Janik, M A; Jayarathna, P H S Y; Jena, C; Jena, S; Jimenez Bustamante, R T; Jones, P G; Jung, H; Jusko, A; Kadyshevskiy, V; Kalcher, S; Kalinak, P; Kalweit, A; Kamin, J; Kang, J H; Kaplin, V; Kar, S; Karasu Uysal, A; Karavichev, O; Karavicheva, T; Karpechev, E; Kebschull, U; Keidel, R; Keijdener, D L D; Keil Svn, M; Khan, M M; Khan, P; Khan, S A; Khanzadeev, A; Kharlov, Y; Kileng, B; Kim, B; Kim, D W; Kim, D J; Kim, J S; Kim, M; Kim, M; Kim, S; Kim, T; Kirsch, S; Kisel, I; Kiselev, S; Kisiel, A; Kiss, G; Klay, J L; Klein, J; Klein-Bösing, C; Kluge, A; Knichel, M L; Knospe, A G; Kobdaj, C; Kofarago, M; Köhler, M K; Kollegger, T; Kolojvari, A; Kondratiev, V; Kondratyeva, N; Konevskikh, A; Kovalenko, V; Kowalski, M; Kox, S; Koyithatta Meethaleveedu, G; Kral, J; Králik, I; Kravčáková, A; Krelina, M; Kretz, M; Krivda, M; Krizek, F; Kryshen, E; Krzewicki, M; Kučera, V; Kucheriaev, Y; Kugathasan, T; Kuhn, C; Kuijer, P G; Kulakov, I; Kumar, J; Kurashvili, P; Kurepin, A; Kurepin, A B; Kuryakin, A; Kushpil, S; Kweon, M J; Kwon, Y; Ladron de Guevara, P; Lagana Fernandes, C; Lakomov, I; Langoy, R; Lara, C; Lardeux, A; Lattuca, A; La Pointe, S L; La Rocca, P; Lea, R; Leardini, L; Lee, G R; Legrand, I; Lehnert, J; Lemmon, R C; Lenti, V; Leogrande, E; Leoncino, M; León Monzón, I; Lévai, P; Li, S; Lien, J; Lietava, R; Lindal, S; Lindenstruth, V; Lippmann, C; Lisa, M A; Ljunggren, H M; Lodato, D F; Loenne, P I; Loggins, V R; Loginov, V; Lohner, D; Loizides, C; Lopez, X; López Torres, E; Lu, X-G; Luettig, P; Lunardon, M; Luparello, G; Ma, R; Maevskaya, A; Mager, M; Mahapatra, D P; Mahmood, S M; Maire, A; Majka, R D; Malaev, M; Maldonado Cervantes, I; Malinina, L; Mal'Kevich, D; Malzacher, P; Mamonov, A; Manceau, L; Manko, V; Manso, F; Manzari, V; Marchisone, M; Mareš, J; Margagliotti, G V; Margotti, A; Marín, A; Markert, C; Marquard, M; Martashvili, I; Martin, N A; Martinengo, P; Martínez, M I; Martínez García, G; Martin Blanco, J; Martynov, Y; Mas, A; Masciocchi, S; Masera, M; Masoni, A; Massacrier, L; Mastroserio, A; Matyja, A; Mayer, C; Mazer, J; Mazzoni, M A; Meddi, F; Menchaca-Rocha, A; Meninno, E; Mercado Pérez, J; Meres, M; Miake, Y; Mikhaylov, K; Milano, L; Milosevic, J; Mischke, A; Mishra, A N; Miśkowiec, D; Mitra, J; Mitu, C M; Mlynarz, J; Mohammadi, N; Mohanty, B; Molnar, L; Montaño Zetina, L; Montes, E; Morando, M; Moreira De Godoy, D A; Moretto, S; Morreale, A; Morsch, A; Muccifora, V; Mudnic, E; Mühlheim, D; Muhuri, S; Mukherjee, M; Müller, H; Munhoz, M G; Murray, S; Musa, L; Musinsky, J; Nandi, B K; Nania, R; Nappi, E; Nattrass, C; Nayak, K; Nayak, T K; Nazarenko, S; Nedosekin, A; Nicassio, M; Niculescu, M; Nielsen, B S; Nikolaev, S; Nikulin, S; Nikulin, V; Nilsen, B S; Noferini, F; Nomokonov, P; Nooren, G; Norman, J; Nyanin, A; Nystrand, J; Oeschler, H; Oh, S; Oh, S K; Okatan, A; Olah, L; Oleniacz, J; Oliveira Da Silva, A C; Onderwaater, J; Oppedisano, C; Ortiz Velasquez, A; Oskarsson, A; Otwinowski, J; Oyama, K; Ozdemir, M; Sahoo, P; Pachmayer, Y; Pachr, M; Pagano, P; Paić, G; Painke, F; Pajares, C; Pal, S K; Palmeri, A; Pant, D; Papikyan, V; Pappalardo, G S; Pareek, P; Park, W J; Parmar, S; Passfeld, A; Patalakha, D I; Paticchio, V; Paul, B; Pawlak, T; Peitzmann, T; Pereira Da Costa, H; Pereira De Oliveira Filho, E; Peresunko, D; Pérez Lara, C E; Pesci, A; Peskov, V; Pestov, Y; Petráček, V; Petran, M; Petris, M; Petrovici, M; Petta, C; Piano, S; Pikna, M; Pillot, P; Pinazza, O; Pinsky, L; Piyarathna, D B; Płoskoń, M; Planinic, M; Pluta, J; Pochybova, S; Podesta-Lerma, P L M; Poghosyan, M G; Pohjoisaho, E H O; Polichtchouk, B; Poljak, N; Pop, A; Porteboeuf-Houssais, S; Porter, J; Potukuchi, B; Prasad, S K; Preghenella, R; Prino, F; Pruneau, C A; Pshenichnov, I; Puddu, G; Pujahari, P; Punin, V; Putschke, J; Qvigstad, H; Rachevski, A; Raha, S; Rak, J; Rakotozafindrabe, A; Ramello, L; Raniwala, R; Raniwala, S; Räsänen, S S; Rascanu, B T; Rathee, D; Rauf, A W; Razazi, V; Read, K F; Real, J S; Redlich, K; Reed, R J; Rehman, A; Reichelt, P; Reicher, M; Reidt, F; Renfordt, R; Reolon, A R; Reshetin, A; Rettig, F; Revol, J-P; Reygers, K; Riabov, V; Ricci, R A; Richert, T; Richter, M; Riedler, P; Riegler, W; Riggi, F; Rivetti, A; Rocco, E; Rodríguez Cahuantzi, M; Rodriguez Manso, A; Røed, K; Rogochaya, E; Rohni, S; Rohr, D; Röhrich, D; Romita, R; Ronchetti, F; Ronflette, L; Rosnet, P; Rossi, A; Roukoutakis, F; Roy, A; Roy, C; Roy, P; Rubio Montero, A J; Rui, R; Russo, R; Ryabinkin, E; Ryabov, Y; Rybicki, A; Sadovsky, S; Šafařík, K; Sahlmuller, B; Sahoo, R; Sahu, P K; Saini, J; Sakai, S; Salgado, C A; Salzwedel, J; Sambyal, S; Samsonov, V; Sanchez Castro, X; Sánchez Rodríguez, F J; Šándor, L; Sandoval, A; Sano, M; Santagati, G; Sarkar, D; Scapparone, E; Scarlassara, F; Scharenberg, R P; Schiaua, C; Schicker, R; Schmidt, C; Schmidt, H R; Schuchmann, S; Schukraft, J; Schulc, M; Schuster, T; Schutz, Y; Schwarz, K; Schweda, K; Scioli, G; Scomparin, E; Scott, R; Segato, G; Seger, J E; Sekiguchi, Y; Selyuzhenkov, I; Seo, J; Serradilla, E; Sevcenco, A; Shabetai, A; Shabratova, G; Shahoyan, R; Shangaraev, A; Sharma, N; Sharma, S; Shigaki, K; Shtejer, K; Sibiriak, Y; Siddhanta, S; Siemiarczuk, T; Silvermyr, D; Silvestre, C; Simatovic, G; Singaraju, R; Singh, R; Singha, S; Singhal, V; Sinha, B C; Sinha, T; Sitar, B; Sitta, M; Skaali, T B; Skjerdal, K; Slupecki, M; Smirnov, N; Snellings, R J M; Søgaard, C; Soltz, R; Song, J; Song, M; Soramel, F; Sorensen, S; Spacek, M; Spiriti, E; Sputowska, I; Spyropoulou-Stassinaki, M; Srivastava, B K; Stachel, J; Stan, I; Stefanek, G; Steinpreis, M; Stenlund, E; Steyn, G; Stiller, J H; Stocco, D; Stolpovskiy, M; Strmen, P; Suaide, A A P; Sugitate, T; Suire, C; Suleymanov, M; Sultanov, R; Šumbera, M; Susa, T; Symons, T J M; Szabo, A; Szanto de Toledo, A; Szarka, I; Szczepankiewicz, A; Szymanski, M; Takahashi, J; Tangaro, M A; Tapia Takaki, J D; Tarantola Peloni, A; Tarazona Martinez, A; Tarzila, M G; Tauro, A; Tejeda Muñoz, G; Telesca, A; Terrevoli, C; Thäder, J; Thomas, D; Tieulent, R; Timmins, A R; Toia, A; Trubnikov, V; Trzaska, W H; Tsuji, T; Tumkin, A; Turrisi, R; Tveter, T S; Ullaland, K; Uras, A; Usai, G L; Vajzer, M; Vala, M; Valencia Palomo, L; Vallero, S; Vande Vyvre, P; Van Der Maarel, J; Van Hoorne, J W; van Leeuwen, M; Vargas, A; Vargyas, M; Varma, R; Vasileiou, M; Vasiliev, A; Vechernin, V; Veldhoen, M; Velure, A; Venaruzzo, M; Vercellin, E; Vergara Limón, S; Vernet, R; Verweij, M; Vickovic, L; Viesti, G; Viinikainen, J; Vilakazi, Z; Villalobos Baillie, O; Vinogradov, A; Vinogradov, L; Vinogradov, Y; Virgili, T; Viyogi, Y P; Vodopyanov, A; Völkl, M A; Voloshin, K; Voloshin, S A; Volpe, G; von Haller, B; Vorobyev, I; Vranic, D; Vrláková, J; Vulpescu, B; Vyushin, A; Wagner, B; Wagner, J; Wagner, V; Wang, M; Wang, Y; Watanabe, D; Weber, M; Wessels, J P; Westerhoff, U; Wiechula, J; Wikne, J; Wilde, M; Wilk, G; Wilkinson, J; Williams, M C S; Windelband, B; Winn, M; Yaldo, C G; Yamaguchi, Y; Yang, H; Yang, P; Yang, S; Yano, S; Yasnopolskiy, S; Yi, J; Yin, Z; Yoo, I-K; Yushmanov, I; Zaccolo, V; Zach, C; Zaman, A; Zampolli, C; Zaporozhets, S; Zarochentsev, A; Závada, P; Zaviyalov, N; Zbroszczyk, H; Zgura, I S; Zhalov, M; Zhang, H; Zhang, X; Zhang, Y; Zhao, C; Zhigareva, N; Zhou, D; Zhou, F; Zhou, Y; Zhou, Zhuo; Zhu, H; Zhu, J; Zhu, X; Zichichi, A; Zimmermann, A; Zimmermann, M B; Zinovjev, G; Zoccarato, Y; Zyzak, M

    2014-12-01

    We present the first measurement at the LHC of exclusive J/ψ photoproduction off protons, in ultraperipheral proton-lead collisions at sqrt[s_{NN}]=5.02  TeV. Events are selected with a dimuon pair produced either in the rapidity interval, in the laboratory frame, 2.5photoproduction cross section in γp energies from about 20 to 700 GeV, or equivalently, from Bjorken x scaling variable between ∼2×10^{-2} and ∼2×10^{-5}, thus indicating no significant change in the gluon density behavior of the proton between HERA and LHC energies.

  9. Photoproduction of 7pi0 on hydrogen with CLAS from 1.1 GeV - 5.45 GeV using e+e --gamma decay

    NASA Astrophysics Data System (ADS)

    Kunkel, Michael C.

    Photoproduction of the pi0 meson was studied using the CLAS detector at the Thomas Jefferson National Accelerator Facility using tagged incident photon energies spanning the range Egamma = 1.1 GeV - 5.45 GeV. The measurement is performed on a liquid hydrogen target in the reaction gammap → pe +e--(gamma). The final state of the reaction is the sum of two subprocesses for pi0 decay, the Dalitz decay mode of gamma0 → e +e--gamma and conversion mode where one photon from pi0 → gammagamma decay is converted into a e+e -- pair. This specific final state reaction avoided limitations caused by single prompt track triggering, while the span of incident photon energies allowed for measurements of gamma0 photoproduction to a domain never systematically measured before. We report the measurement of the gamma0 differential cross sections dsigma/dO and dsigma/dt. The angular distributions agree well with the SAID parametrization for incident beam energies below 3 GeV. As a result with this new data, the chi2/p.d.f. of the global fit in the SAID parametrization improved to 3.1 from 3.7. For incident beam energies greater than 3 GeV a comparison of a model based on Generalized Parton Distributions (GPD) with experimental data shows significant discrepancy, requiring further model developments to describe the data.

  10. Exclusive J/ψ photoproduction off protons in ultraperipheral p-Pb collisions at √(s(NN))=5.02 TeV.

    PubMed

    Abelev, B; Adam, J; Adamová, D; Aggarwal, M M; Aglieri Rinella, G; Agnello, M; Agostinelli, A; Agrawal, N; Ahammed, Z; Ahmad, N; Ahmed, I; Ahn, S U; Ahn, S A; Aimo, I; Aiola, S; Ajaz, M; Akindinov, A; Alam, S N; Aleksandrov, D; Alessandro, B; Alexandre, D; Alici, A; Alkin, A; Alme, J; Alt, T; Altinpinar, S; Altsybeev, I; Alves Garcia Prado, C; Andrei, C; Andronic, A; Anguelov, V; Anielski, J; Antičić, T; Antinori, F; Antonioli, P; Aphecetche, L; Appelshäuser, H; Arcelli, S; Armesto, N; Arnaldi, R; Aronsson, T; Arsene, I C; Arslandok, M; Augustinus, A; Averbeck, R; Awes, T C; Azmi, M D; Bach, M; Badalà, A; Baek, Y W; Bagnasco, S; Bailhache, R; Bala, R; Baldisseri, A; Baltasar Dos Santos Pedrosa, F; Baral, R C; Barbera, R; Barile, F; Barnaföldi, G G; Barnby, L S; Barret, V; Bartke, J; Basile, M; Bastid, N; Basu, S; Bathen, B; Batigne, G; Batista Camejo, A; Batyunya, B; Batzing, P C; Baumann, C; Bearden, I G; Beck, H; Bedda, C; Behera, N K; Belikov, I; Bellini, F; Bellwied, R; Belmont-Moreno, E; Belmont, R; Belyaev, V; Bencedi, G; Beole, S; Berceanu, I; Bercuci, A; Berdnikov, Y; Berenyi, D; Berger, M E; Bertens, R A; Berzano, D; Betev, L; Bhasin, A; Bhat, I R; Bhati, A K; Bhattacharjee, B; Bhom, J; Bianchi, L; Bianchi, N; Bianchin, C; Bielčík, J; Bielčíková, J; Bilandzic, A; Bjelogrlic, S; Blanco, F; Blau, D; Blume, C; Bock, F; Bogdanov, A; Bøggild, H; Bogolyubsky, M; Böhmer, F V; Boldizsár, L; Bombara, M; Book, J; Borel, H; Borissov, A; Bossú, F; Botje, M; Botta, E; Böttger, S; Braun-Munzinger, P; Bregant, M; Breitner, T; Broker, T A; Browning, T A; Broz, M; Bruna, E; Bruno, G E; Budnikov, D; Buesching, H; Bufalino, S; Buncic, P; Busch, O; Buthelezi, Z; Caffarri, D; Cai, X; Caines, H; Calero Diaz, L; Caliva, A; Calvo Villar, E; Camerini, P; Carena, F; Carena, W; Castillo Castellanos, J; Casula, E A R; Catanescu, V; Cavicchioli, C; Ceballos Sanchez, C; Cepila, J; Cerello, P; Chang, B; Chapeland, S; Charvet, J L; Chattopadhyay, S; Chattopadhyay, S; Chelnokov, V; Cherney, M; Cheshkov, C; Cheynis, B; Chibante Barroso, V; Chinellato, D D; Chochula, P; Chojnacki, M; Choudhury, S; Christakoglou, P; Christensen, C H; Christiansen, P; Chujo, T; Chung, S U; Cicalo, C; Cifarelli, L; Cindolo, F; Cleymans, J; Colamaria, F; Colella, D; Collu, A; Colocci, M; Conesa Balbastre, G; Conesa Del Valle, Z; Connors, M E; Contreras, J G; Cormier, T M; Corrales Morales, Y; Cortese, P; Cortés Maldonado, I; Cosentino, M R; Costa, F; Crochet, P; Cruz Albino, R; Cuautle, E; Cunqueiro, L; Dainese, A; Dang, R; Danu, A; Das, D; Das, I; Das, K; Das, S; Dash, A; Dash, S; De, S; Delagrange, H; Deloff, A; Dénes, E; D'Erasmo, G; De Caro, A; de Cataldo, G; de Cuveland, J; De Falco, A; De Gruttola, D; De Marco, N; De Pasquale, S; de Rooij, R; Diaz Corchero, M A; Dietel, T; Dillenseger, P; Divià, R; Di Bari, D; Di Liberto, S; Di Mauro, A; Di Nezza, P; Djuvsland, Ø; Dobrin, A; Dobrowolski, T; Domenicis Gimenez, D; Dönigus, B; Dordic, O; Dørheim, S; Dubey, A K; Dubla, A; Ducroux, L; Dupieux, P; Dutta Majumdar, A K; Hilden, T E; Ehlers, R J; Elia, D; Engel, H; Erazmus, B; Erdal, H A; Eschweiler, D; Espagnon, B; Esposito, M; Estienne, M; Esumi, S; Evans, D; Evdokimov, S; Fabris, D; Faivre, J; Falchieri, D; Fantoni, A; Fasel, M; Fehlker, D; Feldkamp, L; Felea, D; Feliciello, A; Feofilov, G; Ferencei, J; Fernández Téllez, A; Ferreiro, E G; Ferretti, A; Festanti, A; Figiel, J; Figueredo, M A S; Filchagin, S; Finogeev, D; Fionda, F M; Fiore, E M; Floratos, E; Floris, M; Foertsch, S; Foka, P; Fokin, S; Fragiacomo, E; Francescon, A; Frankenfeld, U; Fuchs, U; Furget, C; Furs, A; Fusco Girard, M; Gaardhøje, J J; Gagliardi, M; Gago, A M; Gallio, M; Gangadharan, D R; Ganoti, P; Gao, C; Garabatos, C; Garcia-Solis, E; Gargiulo, C; Garishvili, I; Gerhard, J; Germain, M; Gheata, A; Gheata, M; Ghidini, B; Ghosh, P; Ghosh, S K; Gianotti, P; Giubellino, P; Gladysz-Dziadus, E; Glässel, P; Gomez Ramirez, A; González-Zamora, P; Gorbunov, S; Görlich, L; Gotovac, S; Graczykowski, L K; Grelli, A; Grigoras, A; Grigoras, C; Grigoriev, V; Grigoryan, A; Grigoryan, S; Grinyov, B; Grion, N; Grosse-Oetringhaus, J F; Grossiord, J-Y; Grosso, R; Guber, F; Guernane, R; Guerzoni, B; Guilbaud, M; Gulbrandsen, K; Gulkanyan, H; Gumbo, M; Gunji, T; Gupta, A; Gupta, R; Khan, K H; Haake, R; Haaland, Ø; Hadjidakis, C; Haiduc, M; Hamagaki, H; Hamar, G; Hanratty, L D; Hansen, A; Harris, J W; Hartmann, H; Harton, A; Hatzifotiadou, D; Hayashi, S; Heckel, S T; Heide, M; Helstrup, H; Herghelegiu, A; Herrera Corral, G; Hess, B A; Hetland, K F; Hippolyte, B; Hladky, J; Hristov, P; Huang, M; Humanic, T J; Hussain, N; Hutter, D; Hwang, D S; Ilkaev, R; Ilkiv, I; Inaba, M; Innocenti, G M; Ionita, C; Ippolitov, M; Irfan, M; Ivanov, M; Ivanov, V; Jachołkowski, A; Jacobs, P M; Jahnke, C; Jang, H J; Janik, M A; Jayarathna, P H S Y; Jena, C; Jena, S; Jimenez Bustamante, R T; Jones, P G; Jung, H; Jusko, A; Kadyshevskiy, V; Kalcher, S; Kalinak, P; Kalweit, A; Kamin, J; Kang, J H; Kaplin, V; Kar, S; Karasu Uysal, A; Karavichev, O; Karavicheva, T; Karpechev, E; Kebschull, U; Keidel, R; Keijdener, D L D; Keil Svn, M; Khan, M M; Khan, P; Khan, S A; Khanzadeev, A; Kharlov, Y; Kileng, B; Kim, B; Kim, D W; Kim, D J; Kim, J S; Kim, M; Kim, M; Kim, S; Kim, T; Kirsch, S; Kisel, I; Kiselev, S; Kisiel, A; Kiss, G; Klay, J L; Klein, J; Klein-Bösing, C; Kluge, A; Knichel, M L; Knospe, A G; Kobdaj, C; Kofarago, M; Köhler, M K; Kollegger, T; Kolojvari, A; Kondratiev, V; Kondratyeva, N; Konevskikh, A; Kovalenko, V; Kowalski, M; Kox, S; Koyithatta Meethaleveedu, G; Kral, J; Králik, I; Kravčáková, A; Krelina, M; Kretz, M; Krivda, M; Krizek, F; Kryshen, E; Krzewicki, M; Kučera, V; Kucheriaev, Y; Kugathasan, T; Kuhn, C; Kuijer, P G; Kulakov, I; Kumar, J; Kurashvili, P; Kurepin, A; Kurepin, A B; Kuryakin, A; Kushpil, S; Kweon, M J; Kwon, Y; Ladron de Guevara, P; Lagana Fernandes, C; Lakomov, I; Langoy, R; Lara, C; Lardeux, A; Lattuca, A; La Pointe, S L; La Rocca, P; Lea, R; Leardini, L; Lee, G R; Legrand, I; Lehnert, J; Lemmon, R C; Lenti, V; Leogrande, E; Leoncino, M; León Monzón, I; Lévai, P; Li, S; Lien, J; Lietava, R; Lindal, S; Lindenstruth, V; Lippmann, C; Lisa, M A; Ljunggren, H M; Lodato, D F; Loenne, P I; Loggins, V R; Loginov, V; Lohner, D; Loizides, C; Lopez, X; López Torres, E; Lu, X-G; Luettig, P; Lunardon, M; Luparello, G; Ma, R; Maevskaya, A; Mager, M; Mahapatra, D P; Mahmood, S M; Maire, A; Majka, R D; Malaev, M; Maldonado Cervantes, I; Malinina, L; Mal'Kevich, D; Malzacher, P; Mamonov, A; Manceau, L; Manko, V; Manso, F; Manzari, V; Marchisone, M; Mareš, J; Margagliotti, G V; Margotti, A; Marín, A; Markert, C; Marquard, M; Martashvili, I; Martin, N A; Martinengo, P; Martínez, M I; Martínez García, G; Martin Blanco, J; Martynov, Y; Mas, A; Masciocchi, S; Masera, M; Masoni, A; Massacrier, L; Mastroserio, A; Matyja, A; Mayer, C; Mazer, J; Mazzoni, M A; Meddi, F; Menchaca-Rocha, A; Meninno, E; Mercado Pérez, J; Meres, M; Miake, Y; Mikhaylov, K; Milano, L; Milosevic, J; Mischke, A; Mishra, A N; Miśkowiec, D; Mitra, J; Mitu, C M; Mlynarz, J; Mohammadi, N; Mohanty, B; Molnar, L; Montaño Zetina, L; Montes, E; Morando, M; Moreira De Godoy, D A; Moretto, S; Morreale, A; Morsch, A; Muccifora, V; Mudnic, E; Mühlheim, D; Muhuri, S; Mukherjee, M; Müller, H; Munhoz, M G; Murray, S; Musa, L; Musinsky, J; Nandi, B K; Nania, R; Nappi, E; Nattrass, C; Nayak, K; Nayak, T K; Nazarenko, S; Nedosekin, A; Nicassio, M; Niculescu, M; Nielsen, B S; Nikolaev, S; Nikulin, S; Nikulin, V; Nilsen, B S; Noferini, F; Nomokonov, P; Nooren, G; Norman, J; Nyanin, A; Nystrand, J; Oeschler, H; Oh, S; Oh, S K; Okatan, A; Olah, L; Oleniacz, J; Oliveira Da Silva, A C; Onderwaater, J; Oppedisano, C; Ortiz Velasquez, A; Oskarsson, A; Otwinowski, J; Oyama, K; Ozdemir, M; Sahoo, P; Pachmayer, Y; Pachr, M; Pagano, P; Paić, G; Painke, F; Pajares, C; Pal, S K; Palmeri, A; Pant, D; Papikyan, V; Pappalardo, G S; Pareek, P; Park, W J; Parmar, S; Passfeld, A; Patalakha, D I; Paticchio, V; Paul, B; Pawlak, T; Peitzmann, T; Pereira Da Costa, H; Pereira De Oliveira Filho, E; Peresunko, D; Pérez Lara, C E; Pesci, A; Peskov, V; Pestov, Y; Petráček, V; Petran, M; Petris, M; Petrovici, M; Petta, C; Piano, S; Pikna, M; Pillot, P; Pinazza, O; Pinsky, L; Piyarathna, D B; Płoskoń, M; Planinic, M; Pluta, J; Pochybova, S; Podesta-Lerma, P L M; Poghosyan, M G; Pohjoisaho, E H O; Polichtchouk, B; Poljak, N; Pop, A; Porteboeuf-Houssais, S; Porter, J; Potukuchi, B; Prasad, S K; Preghenella, R; Prino, F; Pruneau, C A; Pshenichnov, I; Puddu, G; Pujahari, P; Punin, V; Putschke, J; Qvigstad, H; Rachevski, A; Raha, S; Rak, J; Rakotozafindrabe, A; Ramello, L; Raniwala, R; Raniwala, S; Räsänen, S S; Rascanu, B T; Rathee, D; Rauf, A W; Razazi, V; Read, K F; Real, J S; Redlich, K; Reed, R J; Rehman, A; Reichelt, P; Reicher, M; Reidt, F; Renfordt, R; Reolon, A R; Reshetin, A; Rettig, F; Revol, J-P; Reygers, K; Riabov, V; Ricci, R A; Richert, T; Richter, M; Riedler, P; Riegler, W; Riggi, F; Rivetti, A; Rocco, E; Rodríguez Cahuantzi, M; Rodriguez Manso, A; Røed, K; Rogochaya, E; Rohni, S; Rohr, D; Röhrich, D; Romita, R; Ronchetti, F; Ronflette, L; Rosnet, P; Rossi, A; Roukoutakis, F; Roy, A; Roy, C; Roy, P; Rubio Montero, A J; Rui, R; Russo, R; Ryabinkin, E; Ryabov, Y; Rybicki, A; Sadovsky, S; Šafařík, K; Sahlmuller, B; Sahoo, R; Sahu, P K; Saini, J; Sakai, S; Salgado, C A; Salzwedel, J; Sambyal, S; Samsonov, V; Sanchez Castro, X; Sánchez Rodríguez, F J; Šándor, L; Sandoval, A; Sano, M; Santagati, G; Sarkar, D; Scapparone, E; Scarlassara, F; Scharenberg, R P; Schiaua, C; Schicker, R; Schmidt, C; Schmidt, H R; Schuchmann, S; Schukraft, J; Schulc, M; Schuster, T; Schutz, Y; Schwarz, K; Schweda, K; Scioli, G; Scomparin, E; Scott, R; Segato, G; Seger, J E; Sekiguchi, Y; Selyuzhenkov, I; Seo, J; Serradilla, E; Sevcenco, A; Shabetai, A; Shabratova, G; Shahoyan, R; Shangaraev, A; Sharma, N; Sharma, S; Shigaki, K; Shtejer, K; Sibiriak, Y; Siddhanta, S; Siemiarczuk, T; Silvermyr, D; Silvestre, C; Simatovic, G; Singaraju, R; Singh, R; Singha, S; Singhal, V; Sinha, B C; Sinha, T; Sitar, B; Sitta, M; Skaali, T B; Skjerdal, K; Slupecki, M; Smirnov, N; Snellings, R J M; Søgaard, C; Soltz, R; Song, J; Song, M; Soramel, F; Sorensen, S; Spacek, M; Spiriti, E; Sputowska, I; Spyropoulou-Stassinaki, M; Srivastava, B K; Stachel, J; Stan, I; Stefanek, G; Steinpreis, M; Stenlund, E; Steyn, G; Stiller, J H; Stocco, D; Stolpovskiy, M; Strmen, P; Suaide, A A P; Sugitate, T; Suire, C; Suleymanov, M; Sultanov, R; Šumbera, M; Susa, T; Symons, T J M; Szabo, A; Szanto de Toledo, A; Szarka, I; Szczepankiewicz, A; Szymanski, M; Takahashi, J; Tangaro, M A; Tapia Takaki, J D; Tarantola Peloni, A; Tarazona Martinez, A; Tarzila, M G; Tauro, A; Tejeda Muñoz, G; Telesca, A; Terrevoli, C; Thäder, J; Thomas, D; Tieulent, R; Timmins, A R; Toia, A; Trubnikov, V; Trzaska, W H; Tsuji, T; Tumkin, A; Turrisi, R; Tveter, T S; Ullaland, K; Uras, A; Usai, G L; Vajzer, M; Vala, M; Valencia Palomo, L; Vallero, S; Vande Vyvre, P; Van Der Maarel, J; Van Hoorne, J W; van Leeuwen, M; Vargas, A; Vargyas, M; Varma, R; Vasileiou, M; Vasiliev, A; Vechernin, V; Veldhoen, M; Velure, A; Venaruzzo, M; Vercellin, E; Vergara Limón, S; Vernet, R; Verweij, M; Vickovic, L; Viesti, G; Viinikainen, J; Vilakazi, Z; Villalobos Baillie, O; Vinogradov, A; Vinogradov, L; Vinogradov, Y; Virgili, T; Viyogi, Y P; Vodopyanov, A; Völkl, M A; Voloshin, K; Voloshin, S A; Volpe, G; von Haller, B; Vorobyev, I; Vranic, D; Vrláková, J; Vulpescu, B; Vyushin, A; Wagner, B; Wagner, J; Wagner, V; Wang, M; Wang, Y; Watanabe, D; Weber, M; Wessels, J P; Westerhoff, U; Wiechula, J; Wikne, J; Wilde, M; Wilk, G; Wilkinson, J; Williams, M C S; Windelband, B; Winn, M; Yaldo, C G; Yamaguchi, Y; Yang, H; Yang, P; Yang, S; Yano, S; Yasnopolskiy, S; Yi, J; Yin, Z; Yoo, I-K; Yushmanov, I; Zaccolo, V; Zach, C; Zaman, A; Zampolli, C; Zaporozhets, S; Zarochentsev, A; Závada, P; Zaviyalov, N; Zbroszczyk, H; Zgura, I S; Zhalov, M; Zhang, H; Zhang, X; Zhang, Y; Zhao, C; Zhigareva, N; Zhou, D; Zhou, F; Zhou, Y; Zhou, Zhuo; Zhu, H; Zhu, J; Zhu, X; Zichichi, A; Zimmermann, A; Zimmermann, M B; Zinovjev, G; Zoccarato, Y; Zyzak, M

    2014-12-01

    We present the first measurement at the LHC of exclusive J/ψ photoproduction off protons, in ultraperipheral proton-lead collisions at sqrt[s_{NN}]=5.02  TeV. Events are selected with a dimuon pair produced either in the rapidity interval, in the laboratory frame, 2.5photoproduction cross section in γp energies from about 20 to 700 GeV, or equivalently, from Bjorken x scaling variable between ∼2×10^{-2} and ∼2×10^{-5}, thus indicating no significant change in the gluon density behavior of the proton between HERA and LHC energies. PMID:25526123

  11. Unexpected photoproduct generated via the acetone-sensitized photolysis of 5-bromo-2'-deoxyuridine in a water/isopropanol solution: experimental and computational studies.

    PubMed

    Polska, Katarzyna; Zielonka, Justyna; Chomicz, Lidia; Czerwicka, Małgorzata; Stepnowski, Piotr; Guzow, Katarzyna; Wiczk, Wiesław; Smużyńska, Maria; Kasprzykowski, Franciszek; Żylicz-Stachula, Agnieszka; Skowron, Piotr; Rak, Janusz

    2010-12-23

    The acetone-sensitized photolysis of 5-bromo-2'-deoxyuridine (5-BrdU) in a water/isopropanol solution with 300 nm photons leads to the formation of 2'-deoxyuridine (dU) and a comparable amount of another photoproduct that has not been reported in the literature so far. The negative and positive mass spectra recorded for this species indicate that they originate from the molecular mass of 286 Da, which corresponds to an adduct of 2'-deoxyuridine and 2-propanol. Quantum chemical calculations carried out at the DFT and TDDFT levels reveal both the structure and the UV spectrum of that adduct. The latter computational characteristic matches well the experimental UV spectrum of the new photoproduct. Our findings indicate that the acetone-sensitized photolysis of 5-BrdU is more complicated than has hitherto been assumed. Nevertheless, since electron transfer is one of the pathways responsible for 5-BrdU decay, acetone-sensitized photolysis of the halogen derivatives of nucleobases could be a convenient tool for studying their radiosensitivity in aqueous solutions. PMID:21117683

  12. Unexpected photoproduct generated via the acetone-sensitized photolysis of 5-bromo-2'-deoxyuridine in a water/isopropanol solution: experimental and computational studies.

    PubMed

    Polska, Katarzyna; Zielonka, Justyna; Chomicz, Lidia; Czerwicka, Małgorzata; Stepnowski, Piotr; Guzow, Katarzyna; Wiczk, Wiesław; Smużyńska, Maria; Kasprzykowski, Franciszek; Żylicz-Stachula, Agnieszka; Skowron, Piotr; Rak, Janusz

    2010-12-23

    The acetone-sensitized photolysis of 5-bromo-2'-deoxyuridine (5-BrdU) in a water/isopropanol solution with 300 nm photons leads to the formation of 2'-deoxyuridine (dU) and a comparable amount of another photoproduct that has not been reported in the literature so far. The negative and positive mass spectra recorded for this species indicate that they originate from the molecular mass of 286 Da, which corresponds to an adduct of 2'-deoxyuridine and 2-propanol. Quantum chemical calculations carried out at the DFT and TDDFT levels reveal both the structure and the UV spectrum of that adduct. The latter computational characteristic matches well the experimental UV spectrum of the new photoproduct. Our findings indicate that the acetone-sensitized photolysis of 5-BrdU is more complicated than has hitherto been assumed. Nevertheless, since electron transfer is one of the pathways responsible for 5-BrdU decay, acetone-sensitized photolysis of the halogen derivatives of nucleobases could be a convenient tool for studying their radiosensitivity in aqueous solutions.

  13. Comparative amperometric study of uptake hydrogenase and hydrogen photoproduction activities between heterocystous cyanobacterium Anabaena cylindrica B629 and nonheterocystous cyanobacterium Oscillatoria sp. strain Miami BG7

    SciTech Connect

    Kumazawa, S.; Mitsui, A.

    1985-08-01

    Heterocystous filamentous cyanobacterium Anabaena cylindrica B629 and nonheterocystous filamentous cyanobacterium Oscillatoria sp. strain Miami BG7 were cultured in media with N/sub 2/ as the sole nitrogen source; and activities of oxygen-dependent hydrogen uptake, photohydrogen production photooxygen evolution, and respiration were compared amperometrically under the same or similar experimental conditions for both strains. Distinct differences in these activities were observed in both strains. The rates of hydrogen photoproduction and hydrogen accumulation were significantly higher in Oscillatoria sp. strain BG7 than in A. cylindrica B629 at every light intensity tested. The major reason for the difference was attributable to the fact that the heterocystous cyanobacterium had a high rate of oxygen-dependent hydrogen consumption activity and the nonheterocystous cyanobacterium did not. The activity of oxygen photoevolution and respiration also contributed to the difference. Oscillatoria sp. strain BG7 had lower O/sub 2/ evolution and higher respiration than did A. cylindrica B629. Thus, the effect of O/sub 2/ on hydrogen photoproduction was minimized in Oscillatoria sp. strain BG7. 32 references, 5 figures.

  14. Polarization phenomena in small-angle photoproduction of e{sup +}e{sup {minus}} pairs and the Gerasimov-Drell-Hearn sum rule

    SciTech Connect

    Lvov, A.I.; Scopetta, S. |; Drechsel, D.; Scherer, S.

    1998-01-01

    Photoproduction of e{sup +}e{sup {minus}} pairs at small angles is investigated as a tool to determine the functions f{sub 1} and f{sub 2} entering the real-photon forward Compton scattering amplitude. The method is based on an interference of the Bethe-Heitler and the virtual Compton scattering mechanisms, generating an azimuthal asymmetry in the e{sup +} versus e{sup {minus}} yield. The general case of a circularly polarized beam and a longitudinally polarized target allows one to determine both the real and imaginary parts of f{sub 1} as well as f{sub 2}. The imaginary part of f{sub 2} requires target polarization only. We calculate cross sections and asymmetries of the reaction p({gamma},e{sup +}e{sup {minus}})p, estimate corrections and backgrounds, and propose suitable kinematical regions to perform the experiment. Our investigation shows that photoproduction of e{sup +}e{sup {minus}}-pairs off the proton and light nuclei may serve as a rather sensitive test of the validity of the Gerasimov-Drell-Hearn sum rule. {copyright} {ital 1998} {ital The American Physical Society}

  15. Identification of halogenated photoproducts generated after ultraviolet-irradiation of parabens and benzoates in water containing chlorine by solid-phase microextraction and gas chromatography-mass spectrometry.

    PubMed

    Alvarez-Rivera, Gerardo; Llompart, Maria; Garcia-Jares, Carmen; Lores, Marta

    2014-07-01

    This work presents a new solid-phase microextraction (SPME)-based approach to investigate the formation of halogenated by-products generated by the UV-induced photodegradation of parabens and their congener benzoates in water containing chlorine. Degradation of parent species, and further identification of their transformation by-products were monitored by gas chromatography coupled to mass spectrometry (GC/MS). In order to improve detectability, SPME was applied as a preconcentration step after UV-irradiation of target preservatives. Experiments performed with dechlorinated water, ultrapure water, and tap water showed that under UV-light, the presence of even low levels of free chlorine, increases the photodegradation rate of target preservatives, enhancing the formation of halogenated photoproducts. Monobrominated, dibrominated and bromochlorinated hydroxybenzoates were identified, and the transformation of benzoates into halogenated parabens was also confirmed. Bromination is expected to occur when free chlorine is present, due to the presence of traces of bromide in water samples. Five halogenated phenols (mainly brominated) were detected as breakdown photoproducts from both families of target preservatives. On the basis of the appearance of the aforementioned by-products, a tentative transformation pathway, consistent with the photoformation-photodecay kinetics of the by-products, is proposed herein for the first time.

  16. Comparative Amperometric Study of Uptake Hydrogenase and Hydrogen Photoproduction Activities between Heterocystous Cyanobacterium Anabaena cylindrica B629 and Nonheterocystous Cyanobacterium Oscillatoria sp. Strain Miami BG7

    PubMed Central

    Kumazawa, S.; Mitsui, A.

    1985-01-01

    Heterocystous filamentous cyanobacterium Anabaena cylindrica B629 and nonheterocystous filamentous cyanobacterium Oscillatoria sp. strain Miami BG7 were cultured in media with N2 as the sole nitrogen source; and activities of oxygen-dependent hydrogen uptake, photohydrogen production, photooxygen evolution, and respiration were compared amperometrically under the same or similar experimental conditions for both strains. Distinct differences in these activities were observed in both strains. The rates of hydrogen photoproduction and hydrogen accumulation were significantly higher in Oscillatoria sp. strain BG7 than in A. cylindrica B629 at every light intensity tested. The major reason for the difference was attributable to the fact that the heterocystous cyanobacterium had a high rate of oxygen-dependent hydrogen consumption activity and the nonheterocystous cyanobacterium did not. The activity of oxygen photoevolution and respiration also contributed to the difference. Oscillatoria sp. strain BG7 had lower O2 evolution and higher respiration than did A. cylindrica B629. Thus, the effect of O2 on hydrogen photoproduction was minimized in Oscillatoria sp. strain BG7. PMID:16346850

  17. Faster DNA Repair of Ultraviolet-Induced Cyclobutane Pyrimidine Dimers and Lower Sensitivity to Apoptosis in Human Corneal Epithelial Cells than in Epidermal Keratinocytes.

    PubMed

    Mallet, Justin D; Dorr, Marie M; Drigeard Desgarnier, Marie-Catherine; Bastien, Nathalie; Gendron, Sébastien P; Rochette, Patrick J

    2016-01-01

    Absorption of UV rays by DNA generates the formation of mutagenic cyclobutane pyrimidine dimers (CPD) and pyrimidine (6-4) pyrimidone photoproducts (6-4PP). These damages are the major cause of skin cancer because in turn, they can lead to signature UV mutations. The eye is exposed to UV light, but the cornea is orders of magnitude less prone to UV-induced cancer. In an attempt to shed light on this paradox, we compared cells of the corneal epithelium and the epidermis for UVB-induced DNA damage frequency, repair and cell death sensitivity. We found similar CPD levels but a 4-time faster UVB-induced CPD, but not 6-4PP, repair and lower UV-induced apoptosis sensitivity in corneal epithelial cells than epidermal. We then investigated levels of DDB2, a UV-induced DNA damage recognition protein mostly impacting CPD repair, XPC, essential for the repair of both CPD and 6-4PP and p53 a protein upstream of the genotoxic stress response. We found more DDB2, XPC and p53 in corneal epithelial cells than in epidermal cells. According to our results analyzing the protein stability of DDB2 and XPC, the higher level of DDB2 and XPC in corneal epithelial cells is most likely due to an increased stability of the protein. Taken together, our results show that corneal epithelial cells have a better efficiency to repair UV-induced mutagenic CPD. On the other hand, they are less prone to UV-induced apoptosis, which could be related to the fact that since the repair is more efficient in the HCEC, the need to eliminate highly damaged cells by apoptosis is reduced. PMID:27611318

  18. CYP2D6*4 Allele Polymorphism Increases the Risk of Parkinson’s Disease: Evidence from Meta-Analysis

    PubMed Central

    Chen, Siyuan; Xie, Yantong; Peng, Qiliu; He, Yu; Deng, Yan; Wang, Jian; Xie, Li; Zeng, Jie; Li, Shan; Qin, Xue

    2013-01-01

    Background Many epidemiological studies have been conducted to explore the association between a single CYP2D6 gene polymorphism and Parkinson’s disease (PD) susceptibility. However, the results remain controversial. Objectives To clarify the effects of a single CYP2D6 gene polymorphism on the risk of PD, a meta-analysis of all available studies relating to CYP2D6*4 polymorphism and the risk of PD was conducted. Methods A comprehensive literature search of PubMed, EMBASE, and the China National Knowledge Infrastructure (CNKI) up to September 1, 2013 was conducted. Data were extracted by two independent authors and pooled odds ratio (OR) with 95% confidence interval (CI) were calculated. Meta-regression, Galbraith plots, subgroup analysis, sensitivity analysis, and publication bias analysis were also performed. Results Twenty-two separate comparisons consisting of 2,629 patients and 3,601 controls were included in our meta-analysis. The pooled analyses showed a significant association between CYP2D6*4G/A polymorphism and PD risk in all of the comparisons (A vs. G allele: OR = 1.28, 95% CI = 1.14–1.43, P = 0.001; AA vs. GG: OR = 1.43, 95% CI = 1.06–1.93, P = 0.018; AG vs. GG: OR = 1.22, 95% CI = 1.06–1.40, P = 0.006; AG+AA vs. GG: OR = 1.26, 95% CI = 1.10–1.44, P = 0.001; AA vs. AG+GG: OR = 1.37, 95% CI = 1.02–1.83, P = 0.036). In subgroup analysis stratified by ethnicity, significant associations were also demonstrated in Caucasians but not in Asians. No significant association was found in subgroup analysis stratified by age of onset or disease form. Conclusions We concluded that the CYP2D6*4G/A polymorphism denotes an increased genetic susceptibility to PD in the overall population, especially in Caucasians. Further large and well-designed studies are needed to confirm this association. PMID:24376807

  19. A 6.4MB duplication of the alpha-synuclein locus causing fronto-temporal dementia and parkinsonism - phenotype-genotype correlations

    PubMed Central

    Kara, Eleanna; Kiely, Aoife P; Proukakis, Christos; Giffin, Nicola; Love, Seth; Hehir, Jason; Rantell, Khadija; Pandraud, Amelie; Hernandez, Dena G; Nacheva, Elizabeth; Pittman, Alan M; Nalls, Mike A; Singleton, Andrew B; Revesz, Tamas; Bhatia, Kailash P; Quinn, Niall; Hardy, John; Holton, Janice L; Houlden, Henry

    2015-01-01

    Importance SNCA locus duplications are associated with variable clinical features and reduced penetrance but the reasons underlying this variability are unknown. Objective 1) To report a novel family carrying a heterozygous 6.4Mb duplication of the SNCA locus with an atypical clinical presentation strongly reminiscent of frontotemporal dementia (FTD) and late-onset pallidopyramidal syndromes. 2) To study phenotype-genotype correlations in SNCA locus duplications. Design, Setting, Participants and Data sources We report the clinical and neuropathologic features of a family carrying a 6.4Mb duplication of the SNCA locus. To identify candidate disease modifiers, we undertake a genetic analysis in the family and conduct statistical analysis on previously published cases carrying SNCA locus duplication using regression modelling with robust standard errors to account for clustering at the family level. Main outcome measures To assess whether length of the SNCA locus duplication influences disease penetrance and severity, and whether extra-duplication factors have a disease-modifying role. Results We identified a large 6.4Mb duplication of the SNCA locus in this family. Neuropathological analysis showed extensive α-synuclein pathology with minimal phospho-tau pathology. Genetic analysis showed an increased burden of PD-related risk factors and the disease-predisposing H1/H1 MAPT haplotype. Statistical analysis of previously published cases suggested that there is a trend towards increasing disease severity and disease penetrance with increasing duplication size. The corresponding odds ratios (95% CI) from the univariate analyses were 1.17 (0.81 to 1.68) and 1.34 (0.78 to 2.31) respectively. Gender was significantly associated with both disease risk and severity; males compared to females had increased disease risk and severity and the corresponding odds ratios (95% CI) from the univariate analyses were 8.36 (1.97 to 35.42) and 5.55 (1.39 to 22.22) respectively

  20. Solvent effects on the oxidation (electron transfer) reaction of [Fe(CN) 6] 4- by [Co(NH 3) 5pz] 3+

    NASA Astrophysics Data System (ADS)

    Muriel, F.; Jiménez, R.; López, M.; Prado-Gotor, R.; Sánchez, F.

    2004-03-01

    Solvent effects on the title reaction were studied in different reaction media constituted by water and organic cosolvents (methanol, tert-butyl alcohol, ethyleneglycol and glucose) at 298.2 K. The results are considered in light of the Marcus-Hush approach for electron transfer reactions. Variations of the electron transfer rate constant are shown to be mainly due to changes in the reaction free energy. On the other hand the energies of the MMCT band, corresponding to the optical electron transfer within the ion pair [Fe(CN) 6] 4-/[Co(NH 3) 5pz] 3+, in the different reaction media, have been obtained. The activation free energies of the thermal electron transfer process have been calculated from the band ( Eop) data, and compared with those obtained from the kinetic study. Quantitative agreement is found between the two series of data. This shows the possibility of estimating activation free energies for electron transfer reactions from static (optical) measurements.

  1. A Chandra Observation of the Luminous Northeastern Rim of the Galactic Supernova Remnant W28 (G6.4-0.1)

    NASA Astrophysics Data System (ADS)

    Pannuti, Thomas

    2016-06-01

    We present an analysis of a pointed observation made of the luminous northeastern rim of the Galactic supernova remnant (SNR) W28 (G6.4-0.1) with the Chandra X-ray Observatory. W28 is the archetype for the class of SNRs known as the mixed-morphology SNRs: sources in this class of objects feature a shell-like morphology with a contrasting center-filled X-ray morphology. Almost unique amongst mixed-morphology SNRs, W28 exhibits a luminous northeastern rim which is detected in the X-ray, optical and radio: this rim is also the site of a vigorous interaction between W28 and adjacent molecular clouds, as evidenced by the high concentration of hydroxyl (OH) masers seen at this rim. Our pointed Chandra observation of this rim is the highest angular X-ray observation made of this feature: initial analysis and results will be presented and discussed.

  2. Viscoelastic Response of the Titanium Alloy Ti-6-4: Experimental Identification of Time- and Rate-Dependent Reversible and Irreversible Deformation Regions

    NASA Technical Reports Server (NTRS)

    Lerch, Bradley A.; Arnold, Steven M.

    2014-01-01

    In support of an effort on damage prognosis, the viscoelastic behavior of Ti-6Al-4V (Ti-6-4) was investigated. This report documents the experimental characterization of this titanium alloy. Various uniaxial tests were conducted to low load levels over the temperature range of 20 to 538 C to define tensile, creep, and relaxation behavior. A range of strain rates (6x10(exp -7) to 0.001/s) were used to document rate effects. All tests were designed to include an unloading portion, followed by a hold time at temperature to allow recovery to occur either at zero stress or strain. The titanium alloy was found to exhibit viscoelastic behavior below the "yield" point and over the entire range of temperatures (although at lower temperatures the magnitude is extremely small). These experimental data will be used for future characterization of a viscoelastic model.

  3. Observation of Secondary Magnetic Transition in Tetragonal YBa2Cu3Ox (6.1≤x≤6.4)

    NASA Astrophysics Data System (ADS)

    Matsumura, Masahiro; Yamagata, Hideki; Yamada, Yoshihiro; Ishida, Kenji; Kitaoka, Yoshio; Asayama, Kunisuke; Takagi, Hidenori; Iwabuchi, Hiroyuki; Uchida, Shin-ichi

    1989-03-01

    Temperature dependence of nuclear resonance spectra for the chain Cu(1) and the plane Cu(2) sites was investigated in the oxygen-deficient tetragonal YBa2Cu3Ox (6.1≤x≤6.4). The divergence of the spin-echo decay rate for the NQR at the Cu(1) site was observed at about 20 K, irrespective of the oxygen concentration, x, in x≥6.2. Below this critical temperature, only the NQR line broadening becomes significant without any resonance shifts. The spin-echo decay rate for the NMR at the Cu(2) site in the sample with x{=}6.2 also increases divergently when the temperature is raised to the critical temperature. These facts directly indicate the secondary transition associated with the moments on the Cu(2) sites at about 20 K, which is well below the Néel temperature. Some discussions are included for the magnetic structure below the transition temperature.

  4. Solar photoproduction of hydrogen. IEA technical report of the IEA Agreement of the Production and Utilization of Hydrogen

    SciTech Connect

    Bolton, J.R.

    1996-09-30

    The report was prepared for the International Energy Agency (IEA) Hydrogen Program and represents the result of subtask C, Annex 10 - Photoproduction of Hydrogen. The concept of using solar energy to drive the conversion of water into hydrogen and oxygen has been examined, from the standpoints of potential and ideal efficiencies, measurement of (and how to calculate) solar hydrogen production efficiencies, a survey of the state-of-the-art, and a technological assessment of various solar hydrogen options. The analysis demonstrates that the ideal limit of the conversion efficiency for 1 sun irradiance is {approximately}31% for a single photosystem scheme and {approximately}42% for a dual photosystem scheme. However, practical considerations indicate that real efficiencies will not likely exceed {approximately}10% and {approximately}16% for single and dual photosystem schemes, respectively. Four types of solar photochemical hydrogen systems have been identified: photochemical systems, semiconductor systems, photobiological systems, and hybrid and other systems. A survey of the state-of-the-art of these four types is presented. The four types (and their subtypes) have also been examined in a technological assessment, where each has been examined as to efficiency, potential for improvement, and long-term functionality. Four solar hydrogen systems have been selected as showing sufficient promise for further research and development: (1) Photovoltaic cells plus an electrolyzer; (2) Photoelectrochemical cells with one or more semiconductor electrodes; (3) Photobiological systems; and (4) Photodegradation systems. The following recommendations were presented for consideration of the IEA: (1) Define and measure solar hydrogen conversion efficiencies as the ratio of the rate of generation of Gibbs energy of dry hydrogen gas (with appropriate corrections for any bias power) to the incident solar power (solar irradiance times the irradiated area); (2) Expand support for pilot

  5. Extraction of the photon beam asymmetry Sigma in pi 0 photoproduction off the proton using the CBELSA/TAPS experiment

    NASA Astrophysics Data System (ADS)

    Sparks, Nathan Andrew

    The CBELSA/TAPS experiment at the electron accelerator ELSA, in Bonn, Germany, was used in order to study the photoproduction of neutral pions off the proton with a linearly polarized photon beam; Neutral pions were reconstructed through their dominant decay mode into two photons. The photons were detected in a barrel/forward electromagnetic calorimeter system which covered 99% of the 4pi solid angle. The Crystal Barrel CsI(Tl) calorimeter detected photons at polar angles from 30° to 168°, while TAPS, a BaF2 spectrometer, covered forward polar angles from 5.8° to 30° and served as a fast trigger; Both calorimeters had complete azimuthal angular coverage. Coherent bremsstrahlung of electrons in a diamond radiator was used to produce a linearly polarized beam of photons with a coherent peak at 1305 or 1610 MeV. The analysis of these two datasets allowed for the measurement of the photon beam asymmetry in the beam photon energy range of 920 to 1680 MeV. For the first time, these results cover the very forward polar angles of the neutral pion. The measurements are compared to the SAID, MAID, and BnGa models and to previous measurements. These new measurements of the photon beam asymmetry contribute to the ongoing experimentally-driven exploration of the N and Delta resonances. The study of strange baryons provides a link between the strong interaction physics of the excited nucleons and the heavy flavor baryons. The upcoming GlueX experiment at Jefferson Lab is expected to provide an opportunity to examine strange baryons in much greater detail than ever before. GEANT-based Monte Carlo simulations of Cascade baryons at the GlueX experiment were conducted in order to better understand the capabilities of this experiment. A proposal, "An initial study of mesons and baryons containing strange quarks with GlueX", was submitted to the 40th Jefferson Lab Program Advisory Committee (PAC), in part, supported by these Cascade baryon simulations. 200 days of additional beam

  6. Conserved Negative Charges in the N-terminal Tetramerization Domain Mediate Efficient Assembly of Kv2.1 and Kv2.1/Kv6.4 Channels*

    PubMed Central

    Bocksteins, Elke; Labro, Alain J.; Mayeur, Evy; Bruyns, Tine; Timmermans, Jean-Pierre; Adriaensen, Dirk; Snyders, Dirk J.

    2009-01-01

    Voltage-gated potassium (Kv) channels are transmembrane tetramers of individual α-subunits. Eight different Shaker-related Kv subfamilies have been identified in which the tetramerization domain T1, located on the intracellular N terminus, facilitates and controls the assembly of both homo- and heterotetrameric channels. Only the Kv2 α-subunits are able to form heterotetramers with members of the silent Kv subfamilies (Kv5, Kv6, Kv8, and Kv9). The T1 domain contains two subdomains, A and B box, which presumably determine subfamily specificity by preventing incompatible subunits to assemble. In contrast, little is known about the involvement of the A/B linker sequence. Both Kv2 and silent Kv subfamilies contain a fully conserved and negatively charged sequence (CDD) in this linker that is lacking in the other subfamilies. Neutralizing these aspartates in Kv2.1 by mutating them to alanines did not affect the gating properties, but reduced the current density moderately. However, charge reversal arginine substitutions strongly reduced the current density of these homotetrameric mutant Kv2.1 channels and immunocytochemistry confirmed the reduced expression at the plasma membrane. Förster resonance energy transfer measurements using confocal microscopy showed that the latter was not due to impaired trafficking, but to a failure to assemble the tetramer. This was further confirmed with co-immunoprecipitation experiments. The corresponding arginine substitution in Kv6.4 prevented its heterotetrameric interaction with Kv2.1. These results indicate that these aspartates (especially the first one) in the A/B box linker of the T1 domain are required for efficient assembly of both homotetrameric Kv2.1 and heterotetrameric Kv2.1/silent Kv6.4 channels. PMID:19717558

  7. Energy level properties of 4p{sup 6}4d{sup 3}, 4p{sup 6}4d{sup 2}4f, and 4p{sup 5}4d{sup 4} configurations of the W{sup 35+} ion

    SciTech Connect

    Bogdanovich, P. Kisielius, R.

    2014-11-15

    The ab initio quasirelativistic Hartree–Fock method developed specifically for the calculation of spectroscopic parameters of heavy atoms and highly charged ions was used to derive spectral data for the multicharged tungsten ion W{sup 35+}. The configuration interaction method was applied to include the electron-correlation effects. The relativistic effects were taken into account in the Breit–Pauli approximation for quasirelativistic Hartree–Fock radial orbitals. The energy level spectra, radiative lifetimes τ, and Lande g-factors have been calculated for the 4p{sup 6}4d{sup 3}, 4p{sup 6}4d{sup 2}4f, and 4p{sup 5}4d{sup 4} configurations of the W{sup 35+} ion.

  8. Synthesis and crystal structure of Bi{sub 6.4}Pb{sub 0.6}P{sub 2}O{sub 15.2}

    SciTech Connect

    Arumugam, N.; Lynch, V.; Steinfink, H.

    2007-10-15

    Bi{sub 6.4}Pb{sub 0.6}P{sub 2}O{sub 15.2} is a polymorph of structures with the general stoichiometry Bi{sub 6+x}M{sub 1-x}P{sub 2}O{sub 15+y}. However, unlike previously published structures that consist of layers formed by edge sharing OBi{sub 4} tetrahedra bridged by PO{sub 4} and TO{sub 6} (T=transition metal) tetrahedra and octahedra the title compound's structure is more complex. It is monoclinic, C2, a=19.4698(4) A, b=11.3692(3) A, c=16.3809(5) A, {beta}=101.167(1){sup o}, Z=10. Single-crystal X-ray diffraction data were refined by least squares on F{sup 2} converging to R{sub 1}=0.0387, wR{sub 2}=0.0836 for 7023 intensities. The crystal twins by mirror reflection across (001) as the twin plane and twin component 1 equals 0.74(1). Oxygen ions are in tetrahedral coordination to four metal ions and the O(BiPb){sub 4} units share corners to form layers that are part of the three-dimensional framework. Eight oxygen ions form a cube around the two crystallographically independent Pb ions. Pb-O bond lengths vary from 2.265(14) to 2.869(14) A. Pairs of such cubes share an edge to form a Pb{sub 3}O{sub 20} unit. The two oxygen ions from the unshared edges are part of irregular Bi polyhedra. Other oxygen ions of Bi polyhedra are part only of O(BiPb){sub 4} units, and some oxygen ions of the polyhedra are also part of PO{sub 4} tetrahedra. One, two, three and or four PO{sub 4} moieties are connected to the Bi polyhedra. Bi-O bond lengths {<=}3.1 A vary from 2.090(12) to 3.07(3) A. The articulations of Pb cubes, Bi polyhedra and PO{sub 4} tetrahedra link into the three-dimensional structure. - Graphical abstract: View of the structure of Bi{sub 6.4}Pb{sub 0.6}P{sub 2}O{sub 15.2} parallel to the b-axis.

  9. Coherent ψ(2S) photo-production in ultra-peripheral Pbsbnd Pb collisions at √{sNN} = 2.76 TeV

    NASA Astrophysics Data System (ADS)

    Adam, J.; Adamová, D.; Aggarwal, M. M.; Aglieri Rinella, G.; Agnello, M.; Agrawal, N.; Ahammed, Z.; Ahn, S. U.; Aimo, I.; Aiola, S.; Ajaz, M.; Akindinov, A.; Alam, S. N.; Aleksandrov, D.; Alessandro, B.; Alexandre, D.; Alfaro Molina, R.; Alici, A.; Alkin, A.; Alme, J.; Alt, T.; Altinpinar, S.; Altsybeev, I.; Alves Garcia Prado, C.; Andrei, C.; Andronic, A.; Anguelov, V.; Anielski, J.; Antičić, T.; Antinori, F.; Antonioli, P.; Aphecetche, L.; Appelshäuser, H.; Arcelli, S.; Armesto, N.; Arnaldi, R.; Aronsson, T.; Arsene, I. C.; Arslandok, M.; Augustinus, A.; Averbeck, R.; Azmi, M. D.; Bach, M.; Badalà, A.; Baek, Y. W.; Bagnasco, S.; Bailhache, R.; Bala, R.; Baldisseri, A.; Baltasar Dos Santos Pedrosa, F.; Baral, R. C.; Barbano, A. M.; Barbera, R.; Barile, F.; Barnaföldi, G. G.; Barnby, L. S.; Barret, V.; Bartalini, P.; Barth, K.; Bartke, J.; Bartsch, E.; Basile, M.; Bastid, N.; Basu, S.; Bathen, B.; Batigne, G.; Batista Camejo, A.; Batyunya, B.; Batzing, P. C.; Bearden, I. G.; Beck, H.; Bedda, C.; Behera, N. K.; Belikov, I.; Bellini, F.; Bello Martinez, H.; Bellwied, R.; Belmont, R.; Belmont-Moreno, E.; Belyaev, V.; Bencedi, G.; Beole, S.; Berceanu, I.; Bercuci, A.; Berdnikov, Y.; Berenyi, D.; Bertens, R. A.; Berzano, D.; Betev, L.; Bhasin, A.; Bhat, I. R.; Bhati, A. K.; Bhattacharjee, B.; Bhom, J.; Bianchi, L.; Bianchi, N.; Bianchin, C.; Bielčík, J.; Bielčíková, J.; Bilandzic, A.; Biswas, R.; Biswas, S.; Bjelogrlic, S.; Blanco, F.; Blau, D.; Blume, C.; Bock, F.; Bogdanov, A.; Bøggild, H.; Boldizsár, L.; Bombara, M.; Book, J.; Borel, H.; Borissov, A.; Borri, M.; Bossú, F.; Botje, M.; Botta, E.; Böttger, S.; Braun-Munzinger, P.; Bregant, M.; Breitner, T.; Broker, T. A.; Browning, T. A.; Broz, M.; Brucken, E. J.; Bruna, E.; Bruno, G. E.; Budnikov, D.; Buesching, H.; Bufalino, S.; Buncic, P.; Busch, O.; Buthelezi, Z.; Buxton, J. T.; Caffarri, D.; Cai, X.; Caines, H.; Calero Diaz, L.; Caliva, A.; Calvo Villar, E.; Camerini, P.; Carena, F.; Carena, W.; Castillo Castellanos, J.; Castro, A. J.; Casula, E. A. R.; Cavicchioli, C.; Ceballos Sanchez, C.; Cepila, J.; Cerello, P.; Chang, B.; Chapeland, S.; Chartier, M.; Charvet, J. L.; Chattopadhyay, S.; Chattopadhyay, S.; Chelnokov, V.; Cherney, M.; Cheshkov, C.; Cheynis, B.; Chibante Barroso, V.; Chinellato, D. D.; Chochula, P.; Choi, K.; Chojnacki, M.; Choudhury, S.; Christakoglou, P.; Christensen, C. H.; Christiansen, P.; Chujo, T.; Chung, S. U.; Chunhui, Z.; Cicalo, C.; Cifarelli, L.; Cindolo, F.; Cleymans, J.; Colamaria, F.; Colella, D.; Collu, A.; Colocci, M.; Conesa Balbastre, G.; Conesa del Valle, Z.; Connors, M. E.; Contreras, J. G.; Cormier, T. M.; Corrales Morales, Y.; Cortés Maldonado, I.; Cortese, P.; Cosentino, M. R.; Costa, F.; Crochet, P.; Cruz Albino, R.; Cuautle, E.; Cunqueiro, L.; Dahms, T.; Dainese, A.; Danu, A.; Das, D.; Das, I.; Das, S.; Dash, A.; Dash, S.; De, S.; De Caro, A.; de Cataldo, G.; de Cuveland, J.; De Falco, A.; De Gruttola, D.; De Marco, N.; De Pasquale, S.; Deisting, A.; Deloff, A.; Dénes, E.; D'Erasmo, G.; Di Bari, D.; Di Mauro, A.; Di Nezza, P.; Diaz Corchero, M. A.; Dietel, T.; Dillenseger, P.; Divià, R.; Djuvsland, Ø.; Dobrin, A.; Dobrowolski, T.; Domenicis Gimenez, D.; Dönigus, B.; Dordic, O.; Dubey, A. K.; Dubla, A.; Ducroux, L.; Dupieux, P.; Ehlers, R. J.; Elia, D.; Engel, H.; Erazmus, B.; Erhardt, F.; Eschweiler, D.; Espagnon, B.; Estienne, M.; Esumi, S.; Eum, J.; Evans, D.; Evdokimov, S.; Eyyubova, G.; Fabbietti, L.; Fabris, D.; Faivre, J.; Fantoni, A.; Fasel, M.; Feldkamp, L.; Felea, D.; Feliciello, A.; Feofilov, G.; Ferencei, J.; Fernández Téllez, A.; Ferreiro, E. G.; Ferretti, A.; Festanti, A.; Figiel, J.; Figueredo, M. A. S.; Filchagin, S.; Finogeev, D.; Fionda, F. M.; Fiore, E. M.; Fleck, M. G.; Floris, M.; Foertsch, S.; Foka, P.; Fokin, S.; Fragiacomo, E.; Francescon, A.; Frankenfeld, U.; Fuchs, U.; Furget, C.; Furs, A.; Fusco Girard, M.; Gaardhøje, J. J.; Gagliardi, M.; Gago, A. M.; Gallio, M.; Gangadharan, D. R.; Ganoti, P.; Gao, C.; Garabatos, C.; Garcia-Solis, E.; Gargiulo, C.; Gasik, P.; Germain, M.; Gheata, A.; Gheata, M.; Ghosh, P.; Ghosh, S. K.; Gianotti, P.; Giubellino, P.; Giubilato, P.; Gladysz-Dziadus, E.; Glässel, P.; Gomez Ramirez, A.; González-Zamora, P.; Gorbunov, S.; Görlich, L.; Gotovac, S.; Grabski, V.; Graczykowski, L. K.; Grelli, A.; Grigoras, A.; Grigoras, C.; Grigoriev, V.; Grigoryan, A.; Grigoryan, S.; Grinyov, B.; Grion, N.; Grosse-Oetringhaus, J. F.; Grossiord, J.-Y.; Grosso, R.; Guber, F.; Guernane, R.; Guerzoni, B.; Gulbrandsen, K.; Gulkanyan, H.; Gunji, T.; Gupta, A.; Gupta, R.; Haake, R.; Haaland, Ø.; Hadjidakis, C.; Haiduc, M.; Hamagaki, H.; Hamar, G.; Hanratty, L. D.; Hansen, A.; Harris, J. W.; Hartmann, H.; Harton, A.; Hatzifotiadou, D.; Hayashi, S.; Heckel, S. T.; Heide, M.; Helstrup, H.; Herghelegiu, A.; Herrera Corral, G.; Hess, B. A.; Hetland, K. F.; Hilden, T. E.; Hillemanns, H.; Hippolyte, B.; Hristov, P.; Huang, M.; Humanic, T. J.; Hussain, N.; Hussain, T.; Hutter, D.; Hwang, D. S.; Ilkaev, R.; Ilkiv, I.; Inaba, M.; Ionita, C.; Ippolitov, M.; Irfan, M.; Ivanov, M.; Ivanov, V.; Izucheev, V.; Jacobs, P. M.; Jahnke, C.; Jang, H. J.; Janik, M. A.; Jayarathna, P. H. S. Y.; Jena, C.; Jena, S.; Jimenez Bustamante, R. T.; Jones, P. G.; Jung, H.; Jusko, A.; Kalinak, P.; Kalweit, A.; Kamin, J.; Kang, J. H.; Kaplin, V.; Kar, S.; Karasu Uysal, A.; Karavichev, O.; Karavicheva, T.; Karpechev, E.; Kebschull, U.; Keidel, R.; Keijdener, D. L. D.; Keil, M.; Khan, K. H.; Khan, M. M.; Khan, P.; Khan, S. A.; Khanzadeev, A.; Kharlov, Y.; Kileng, B.; Kim, B.; Kim, D. W.; Kim, D. J.; Kim, H.; Kim, J. S.; Kim, M.; Kim, M.; Kim, S.; Kim, T.; Kirsch, S.; Kisel, I.; Kiselev, S.; Kisiel, A.; Kiss, G.; Klay, J. L.; Klein, C.; Klein, J.; Klein-Bösing, C.; Kluge, A.; Knichel, M. L.; Knospe, A. G.; Kobayashi, T.; Kobdaj, C.; Kofarago, M.; Kollegger, T.; Kolojvari, A.; Kondratiev, V.; Kondratyeva, N.; Kondratyuk, E.; Konevskikh, A.; Kouzinopoulos, C.; Kovalenko, O.; Kovalenko, V.; Kowalski, M.; Kox, S.; Koyithatta Meethaleveedu, G.; Kral, J.; Králik, I.; Kravčáková, A.; Krelina, M.; Kretz, M.; Krivda, M.; Krizek, F.; Kryshen, E.; Krzewicki, M.; Kubera, A. M.; Kučera, V.; Kugathasan, T.; Kuhn, C.; Kuijer, P. G.; Kulakov, I.; Kumar, J.; Kumar, L.; Kurashvili, P.; Kurepin, A.; Kurepin, A. B.; Kuryakin, A.; Kushpil, S.; Kweon, M. J.; Kwon, Y.; La Pointe, S. L.; La Rocca, P.; Lagana Fernandes, C.; Lakomov, I.; Langoy, R.; Lara, C.; Lardeux, A.; Lattuca, A.; Laudi, E.; Lea, R.; Leardini, L.; Lee, G. R.; Lee, S.; Legrand, I.; Lemmon, R. C.; Lenti, V.; Leogrande, E.; León Monzón, I.; Leoncino, M.; Lévai, P.; Li, S.; Li, X.; Lien, J.; Lietava, R.; Lindal, S.; Lindenstruth, V.; Lippmann, C.; Lisa, M. A.; Ljunggren, H. M.; Lodato, D. F.; Loenne, P. I.; Loggins, V. R.; Loginov, V.; Loizides, C.; Lopez, X.; López Torres, E.; Lowe, A.; Luettig, P.; Lunardon, M.; Luparello, G.; Luz, P. H. F. N. D.; Maevskaya, A.; Mager, M.; Mahajan, S.; Mahmood, S. M.; Maire, A.; Majka, R. D.; Malaev, M.; Maldonado Cervantes, I.; Malinina, L.; Mal'Kevich, D.; Malzacher, P.; Mamonov, A.; Manceau, L.; Manko, V.; Manso, F.; Manzari, V.; Marchisone, M.; Mareš, J.; Margagliotti, G. V.; Margotti, A.; Margutti, J.; Marín, A.; Markert, C.; Marquard, M.; Martin, N. A.; Martin Blanco, J.; Martinengo, P.; Martínez, M. I.; Martínez García, G.; Martinez Pedreira, M.; Martynov, Y.; Mas, A.; Masciocchi, S.; Masera, M.; Masoni, A.; Massacrier, L.; Mastroserio, A.; Masui, H.; Matyja, A.; Mayer, C.; Mazer, J.; Mazzoni, M. A.; Mcdonald, D.; Meddi, F.; Menchaca-Rocha, A.; Meninno, E.; Mercado Pérez, J.; Meres, M.; Miake, Y.; Mieskolainen, M. M.; Mikhaylov, K.; Milano, L.; Milosevic, J.; Minervini, L. M.; Mischke, A.; Mishra, A. N.; Miśkowiec, D.; Mitra, J.; Mitu, C. M.; Mohammadi, N.; Mohanty, B.; Molnar, L.; Montaño Zetina, L.; Montes, E.; Morando, M.; Moreira De Godoy, D. A.; Moretto, S.; Morreale, A.; Morsch, A.; Muccifora, V.; Mudnic, E.; Mühlheim, D.; Muhuri, S.; Mukherjee, M.; Müller, H.; Mulligan, J. D.; Munhoz, M. G.; Murray, S.; Musa, L.; Musinsky, J.; Nandi, B. K.; Nania, R.; Nappi, E.; Naru, M. U.; Nattrass, C.; Nayak, K.; Nayak, T. K.; Nazarenko, S.; Nedosekin, A.; Nellen, L.; Ng, F.; Nicassio, M.; Niculescu, M.; Niedziela, J.; Nielsen, B. S.; Nikolaev, S.; Nikulin, S.; Nikulin, V.; Noferini, F.; Nomokonov, P.; Nooren, G.; Norman, J.; Nyanin, A.; Nystrand, J.; Oeschler, H.; Oh, S.; Oh, S. K.; Ohlson, A.; Okatan, A.; Okubo, T.; Olah, L.; Oleniacz, J.; Oliveira Da Silva, A. C.; Oliver, M. H.; Onderwaater, J.; Oppedisano, C.; Ortiz Velasquez, A.; Oskarsson, A.; Otwinowski, J.; Oyama, K.; Ozdemir, M.; Pachmayer, Y.; Pagano, P.; Paić, G.; Pajares, C.; Pal, S. K.; Pan, J.; Pandey, A. K.; Pant, D.; Papikyan, V.; Pappalardo, G. S.; Pareek, P.; Park, W. J.; Parmar, S.; Passfeld, A.; Paticchio, V.; Patra, R. N.; Paul, B.; Peitzmann, T.; Pereira Da Costa, H.; Pereira De Oliveira Filho, E.; Peresunko, D.; Pérez Lara, C. E.; Peskov, V.; Pestov, Y.; Petráček, V.; Petrov, V.; Petrovici, M.; Petta, C.; Piano, S.; Pikna, M.; Pillot, P.; Pinazza, O.; Pinsky, L.; Piyarathna, D. B.; Płoskoń, M.; Planinic, M.; Pluta, J.; Pochybova, S.; Podesta-Lerma, P. L. M.; Poghosyan, M. G.; Polichtchouk, B.; Poljak, N.; Poonsawat, W.; Pop, A.; Porteboeuf-Houssais, S.; Porter, J.; Pospisil, J.; Prasad, S. K.; Preghenella, R.; Prino, F.; Pruneau, C. A.; Pshenichnov, I.; Puccio, M.; Puddu, G.; Pujahari, P.; Punin, V.; Putschke, J.; Qvigstad, H.; Rachevski, A.; Raha, S.; Rajput, S.; Rak, J.; Rakotozafindrabe, A.; Ramello, L.; Raniwala, R.; Raniwala, S.; Räsänen, S. S.; Rascanu, B. T.; Rathee, D.; Read, K. F.; Real, J. S.; Redlich, K.; Reed, R. J.; Rehman, A.; Reichelt, P.; Reidt, F.; Ren, X.; Renfordt, R.; Reolon, A. R.; Reshetin, A.; Rettig, F.; Revol, J.-P.; Reygers, K.; Riabov, V.; Ricci, R. A.; Richert, T.; Richter, M.; Riedler, P.; Riegler, W.; Riggi, F.; Ristea, C.; Rivetti, A.; Rocco, E.; Rodríguez Cahuantzi, M.; Rodriguez Manso, A.; Røed, K.; Rogochaya, E.; Rohr, D.; Röhrich, D.; Romita, R.; Ronchetti, F.; Ronflette, L.; Rosnet, P.; Rossi, A.; Roukoutakis, F.; Roy, A.; Roy, C.; Roy, P.; Rubio Montero, A. J.; Rui, R.; Russo, R.; Ryabinkin, E.; Ryabov, Y.; Rybicki, A.; Sadovsky, S.; Šafařík, K.; Sahlmuller, B.; Sahoo, P.; Sahoo, R.; Sahoo, S.; Sahu, P. K.; Saini, J.; Sakai, S.; Saleh, M. A.; Salgado, C. A.; Salzwedel, J.; Sambyal, S.; Samsonov, V.; Sanchez Castro, X.; Šándor, L.; Sandoval, A.; Sano, M.; Santagati, G.; Sarkar, D.; Scapparone, E.; Scarlassara, F.; Scharenberg, R. P.; Schiaua, C.; Schicker, R.; Schmidt, C.; Schmidt, H. R.; Schuchmann, S.; Schukraft, J.; Schulc, M.; Schuster, T.; Schutz, Y.; Schwarz, K.; Schweda, K.; Scioli, G.; Scomparin, E.; Scott, R.; Seeder, K. S.; Seger, J. E.; Sekiguchi, Y.; Selyuzhenkov, I.; Senosi, K.; Seo, J.; Serradilla, E.; Sevcenco, A.; Shabanov, A.; Shabetai, A.; Shadura, O.; Shahoyan, R.; Shangaraev, A.; Sharma, A.; Sharma, N.; Shigaki, K.; Shtejer, K.; Sibiriak, Y.; Siddhanta, S.; Sielewicz, K. M.; Siemiarczuk, T.; Silvermyr, D.; Silvestre, C.; Simatovic, G.; Simonetti, G.; Singaraju, R.; Singh, R.; Singha, S.; Singhal, V.; Sinha, B. C.; Sinha, T.; Sitar, B.; Sitta, M.; Skaali, T. B.; Skjerdal, K.; Slupecki, M.; Smirnov, N.; Snellings, R. J. M.; Snellman, T. W.; Søgaard, C.; Soltz, R.; Song, J.; Song, M.; Song, Z.; Soramel, F.; Sorensen, S.; Spacek, M.; Spiriti, E.; Sputowska, I.; Spyropoulou-Stassinaki, M.; Srivastava, B. K.; Stachel, J.; Stan, I.; Stefanek, G.; Steinpreis, M.; Stenlund, E.; Steyn, G.; Stiller, J. H.; Stocco, D.; Strmen, P.; Suaide, A. A. P.; Sugitate, T.; Suire, C.; Suleymanov, M.; Sultanov, R.; Šumbera, M.; Symons, T. J. M.; Szabo, A.; Szanto de Toledo, A.; Szarka, I.; Szczepankiewicz, A.; Szymanski, M.; Takahashi, J.; Tanaka, N.; Tangaro, M. A.; Tapia Takaki, J. D.; Tarantola Peloni, A.; Tariq, M.; Tarzila, M. G.; Tauro, A.; Tejeda Muñoz, G.; Telesca, A.; Terasaki, K.; Terrevoli, C.; Teyssier, B.; Thäder, J.; Thomas, D.; Tieulent, R.; Timmins, A. R.; Toia, A.; Trogolo, S.; Trubnikov, V.; Trzaska, W. H.; Tsuji, T.; Tumkin, A.; Turrisi, R.; Tveter, T. S.; Ullaland, K.; Uras, A.; Usai, G. L.; Utrobicic, A.; Vajzer, M.; Vala, M.; Valencia Palomo, L.; Vallero, S.; Van Der Maarel, J.; Van Hoorne, J. W.; van Leeuwen, M.; Vanat, T.; Vande Vyvre, P.; Varga, D.; Vargas, A.; Vargyas, M.; Varma, R.; Vasileiou, M.; Vasiliev, A.; Vauthier, A.; Vechernin, V.; Veen, A. M.; Veldhoen, M.; Velure, A.; Venaruzzo, M.; Vercellin, E.; Vergara Limón, S.; Vernet, R.; Verweij, M.; Vickovic, L.; Viesti, G.; Viinikainen, J.; Vilakazi, Z.; Villalobos Baillie, O.; Vinogradov, A.; Vinogradov, L.; Vinogradov, Y.; Virgili, T.; Vislavicius, V.; Viyogi, Y. P.; Vodopyanov, A.; Völkl, M. A.; Voloshin, K.; Voloshin, S. A.; Volpe, G.; von Haller, B.; Vorobyev, I.; Vranic, D.; Vrláková, J.; Vulpescu, B.; Vyushin, A.; Wagner, B.; Wagner, J.; Wang, H.; Wang, M.; Wang, Y.; Watanabe, D.; Weber, M.; Weber, S. G.; Wessels, J. P.; Westerhoff, U.; Wiechula, J.; Wikne, J.; Wilde, M.; Wilk, G.; Wilkinson, J.; Williams, M. C. S.; Windelband, B.; Winn, M.; Yaldo, C. G.; Yamaguchi, Y.; Yang, H.; Yang, P.; Yano, S.; Yin, Z.; Yokoyama, H.; Yoo, I.-K.; Yurchenko, V.; Yushmanov, I.; Zaborowska, A.; Zaccolo, V.; Zaman, A.; Zampolli, C.; Zanoli, H. J. C.; Zaporozhets, S.; Zarochentsev, A.; Závada, P.; Zaviyalov, N.; Zbroszczyk, H.; Zgura, I. S.; Zhalov, M.; Zhang, H.; Zhang, X.; Zhang, Y.; Zhao, C.; Zhigareva, N.; Zhou, D.; Zhou, Y.; Zhou, Z.; Zhu, H.; Zhu, J.; Zhu, X.; Zichichi, A.; Zimmermann, A.; Zimmermann, M. B.; Zinovjev, G.; Zyzak, M.

    2015-12-01

    We have performed the first measurement of the coherent ψ (2 S) photo-production cross section in ultra-peripheral Pbsbnd Pb collisions at the LHC. This charmonium excited state is reconstructed via the ψ (2 S) →l+l- and ψ (2 S) → J / ψπ+π- decays, where the J / ψ decays into two leptons. The analysis is based on an event sample corresponding to an integrated luminosity of about 22 μb-1. The cross section for coherent ψ (2 S) production in the rapidity interval - 0.9 < y < 0.9 is d σψ(2 S)coh/ dy = 0.83 ± 0.19 (stat + syst) mb. The ψ (2 S) to J / ψ coherent cross section ratio is 0.34-0.07+0.08 (stat + syst). The obtained results are compared to predictions from theoretical models.

  10. Coherent ρ 0 photoproduction in ultra-peripheral Pb-Pb collisions at √{s_{NN}}=2.76 TeV

    NASA Astrophysics Data System (ADS)

    Adam, J.; Adamová, D.; Aggarwal, M. M.; Aglieri Rinella, G.; Agnello, M.; Agrawal, N.; Ahammed, Z.; Ahn, S. U.; Aimo, I.; Aiola, S.; Ajaz, M.; Akindinov, A.; Alam, S. N.; Aleksandrov, D.; Alessandro, B.; Alexandre, D.; Alfaro Molina, R.; Alici, A.; Alkin, A.; Alme, J.; Alt, T.; Altinpinar, S.; Altsybeev, I.; Alves Garcia Prado, C.; Andrei, C.; Andronic, A.; Anguelov, V.; Anielski, J.; Antičić, T.; Antinori, F.; Antonioli, P.; Aphecetche, L.; Appelshäuser, H.; Arcelli, S.; Armesto, N.; Arnaldi, R.; Aronsson, T.; Arsene, I. C.; Arslandok, M.; Augustinus, A.; Averbeck, R.; Azmi, M. D.; Bach, M.; Badalà, A.; Baek, Y. W.; Bagnasco, S.; Bailhache, R.; Bala, R.; Baldisseri, A.; Baltasar Dos Santos Pedrosa, F.; Baral, R. C.; Barbano, A. M.; Barbera, R.; Barile, F.; Barnaföldi, G. G.; Barnby, L. S.; Barret, V.; Bartalini, P.; Barth, K.; Bartke, J.; Bartsch, E.; Basile, M.; Bastid, N.; Basu, S.; Bathen, B.; Batigne, G.; Batista Camejo, A.; Batyunya, B.; Batzing, P. C.; Bearden, I. 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T.; Caffarri, D.; Cai, X.; Caines, H.; Calero Diaz, L.; Caliva, A.; Calvo Villar, E.; Camerini, P.; Carena, F.; Carena, W.; Castillo Castellanos, J.; Castro, A. J.; Casula, E. A. R.; Cavicchioli, C.; Ceballos Sanchez, C.; Cepila, J.; Cerello, P.; Chang, B.; Chapeland, S.; Chartier, M.; Charvet, J. L.; Chattopadhyay, S.; Chattopadhyay, S.; Chelnokov, V.; Cherney, M.; Cheshkov, C.; Cheynis, B.; Chibante Barroso, V.; Chinellato, D. D.; Chochula, P.; Choi, K.; Chojnacki, M.; Choudhury, S.; Christakoglou, P.; Christensen, C. H.; Christiansen, P.; Chujo, T.; Chung, S. U.; Chunhui, Z.; Cicalo, C.; Cifarelli, L.; Cindolo, F.; Cleymans, J.; Colamaria, F.; Colella, D.; Collu, A.; Colocci, M.; Conesa Balbastre, G.; Conesa del Valle, Z.; Connors, M. E.; Contreras, J. G.; Cormier, T. M.; Corrales Morales, Y.; Cortés Maldonado, I.; Cortese, P.; Cosentino, M. R.; Costa, F.; Crochet, P.; Cruz Albino, R.; Cuautle, E.; Cunqueiro, L.; Dahms, T.; Dainese, A.; Danu, A.; Das, D.; Das, I.; Das, S.; Dash, A.; Dash, S.; De, S.; De Caro, A.; de Cataldo, G.; de Cuveland, J.; De Falco, A.; De Gruttola, D.; De Marco, N.; De Pasquale, S.; Deisting, A.; Deloff, A.; Dénes, E.; D'Erasmo, G.; Di Bari, D.; Di Mauro, A.; Di Nezza, P.; Diaz Corchero, M. A.; Dietel, T.; Dillenseger, P.; Divià, R.; Djuvsland, Ø.; Dobrin, A.; Dobrowolski, T.; Domenicis Gimenez, D.; Dönigus, B.; Dordic, O.; Dubey, A. K.; Dubla, A.; Ducroux, L.; Dupieux, P.; Ehlers, R. J.; Elia, D.; Engel, H.; Erazmus, B.; Erhardt, F.; Eschweiler, D.; Espagnon, B.; Estienne, M.; Esumi, S.; Eum, J.; Evans, D.; Evdokimov, S.; Eyyubova, G.; Fabbietti, L.; Fabris, D.; Faivre, J.; Fantoni, A.; Fasel, M.; Feldkamp, L.; Felea, D.; Feliciello, A.; Feofilov, G.; Ferencei, J.; Fernández Téllez, A.; Ferreiro, E. G.; Ferretti, A.; Festanti, A.; Figiel, J.; Figueredo, M. A. S.; Filchagin, S.; Finogeev, D.; Fionda, F. M.; Fiore, E. M.; Fleck, M. G.; Floris, M.; Foertsch, S.; Foka, P.; Fokin, S.; Fragiacomo, E.; Francescon, A.; Frankenfeld, U.; Fuchs, U.; Furget, C.; Furs, A.; Fusco Girard, M.; Gaardhøje, J. J.; Gagliardi, M.; Gago, A. M.; Gallio, M.; Gangadharan, D. R.; Ganoti, P.; Gao, C.; Garabatos, C.; Garcia-Solis, E.; Gargiulo, C.; Gasik, P.; Germain, M.; Gheata, A.; Gheata, M.; Ghosh, P.; Ghosh, S. K.; Gianotti, P.; Giubellino, P.; Giubilato, P.; Gladysz-Dziadus, E.; Glässel, P.; Gomez Ramirez, A.; González-Zamora, P.; Gorbunov, S.; Görlich, L.; Gotovac, S.; Grabski, V.; Graczykowski, L. K.; Grelli, A.; Grigoras, A.; Grigoras, C.; Grigoriev, V.; Grigoryan, A.; Grigoryan, S.; Grinyov, B.; Grion, N.; Grosse-Oetringhaus, J. F.; Grossiord, J.-Y.; Grosso, R.; Guber, F.; Guernane, R.; Guerzoni, B.; Gulbrandsen, K.; Gulkanyan, H.; Gunji, T.; Gupta, A.; Gupta, R.; Haake, R.; Haaland, Ø.; Hadjidakis, C.; Haiduc, M.; Hamagaki, H.; Hamar, G.; Hanratty, L. D.; Hansen, A.; Harris, J. W.; Hartmann, H.; Harton, A.; Hatzifotiadou, D.; Hayashi, S.; Heckel, S. T.; Heide, M.; Helstrup, H.; Herghelegiu, A.; Herrera Corral, G.; Hess, B. A.; Hetland, K. F.; Hilden, T. E.; Hillemanns, H.; Hippolyte, B.; Hristov, P.; Huang, M.; Humanic, T. J.; Hussain, N.; Hussain, T.; Hutter, D.; Hwang, D. S.; Ilkaev, R.; Ilkiv, I.; Inaba, M.; Ionita, C.; Ippolitov, M.; Irfan, M.; Ivanov, M.; Ivanov, V.; Izucheev, V.; Jacobs, P. M.; Jahnke, C.; Jang, H. J.; Janik, M. A.; Jayarathna, P. H. S. Y.; Jena, C.; Jena, S.; Jimenez Bustamante, R. T.; Jones, P. G.; Jung, H.; Jusko, A.; Kalinak, P.; Kalweit, A.; Kamin, J.; Kang, J. H.; Kaplin, V.; Kar, S.; Karasu Uysal, A.; Karavichev, O.; Karavicheva, T.; Karpechev, E.; Kebschull, U.; Keidel, R.; Keijdener, D. L. D.; Keil, M.; Khan, K. H.; Khan, M. M.; Khan, P.; Khan, S. A.; Khanzadeev, A.; Kharlov, Y.; Kileng, B.; Kim, B.; Kim, D. W.; Kim, D. J.; Kim, H.; Kim, J. S.; Kim, M.; Kim, M.; Kim, S.; Kim, T.; Kirsch, S.; Kisel, I.; Kiselev, S.; Kisiel, A.; Kiss, G.; Klay, J. L.; Klein, C.; Klein, J.; Klein-Bösing, C.; Kluge, A.; Knichel, M. L.; Knospe, A. G.; Kobayashi, T.; Kobdaj, C.; Kofarago, M.; Kollegger, T.; Kolojvari, A.; Kondratiev, V.; Kondratyeva, N.; Kondratyuk, E.; Konevskikh, A.; Kouzinopoulos, C.; Kovalenko, O.; Kovalenko, V.; Kowalski, M.; Kox, S.; Koyithatta Meethaleveedu, G.; Kral, J.; Králik, I.; Kravčáková, A.; Krelina, M.; Kretz, M.; Krivda, M.; Krizek, F.; Kryshen, E.; Krzewicki, M.; Kubera, A. M.; Kučera, V.; Kugathasan, T.; Kuhn, C.; Kuijer, P. G.; Kulakov, I.; Kumar, J.; Kumar, L.; Kurashvili, P.; Kurepin, A.; Kurepin, A. B.; Kuryakin, A.; Kushpil, S.; Kweon, M. J.; Kwon, Y.; La Pointe, S. L.; La Rocca, P.; Lagana Fernandes, C.; Lakomov, I.; Langoy, R.; Lara, C.; Lardeux, A.; Lattuca, A.; Laudi, E.; Lea, R.; Leardini, L.; Lee, G. R.; Lee, S.; Legrand, I.; Lemmon, R. C.; Lenti, V.; Leogrande, E.; León Monzón, I.; Leoncino, M.; Lévai, P.; Li, S.; Li, X.; Lien, J.; Lietava, R.; Lindal, S.; Lindenstruth, V.; Lippmann, C.; Lisa, M. A.; Ljunggren, H. M.; Lodato, D. F.; Loenne, P. I.; Loggins, V. R.; Loginov, V.; Loizides, C.; Lopez, X.; López Torres, E.; Lowe, A.; Luettig, P.; Lunardon, M.; Luparello, G.; Luz, P. H. F. N. D.; Maevskaya, A.; Mager, M.; Mahajan, S.; Mahmood, S. M.; Maire, A.; Majka, R. D.; Malaev, M.; Maldonado Cervantes, I.; Malinina, L.; Mal'Kevich, D.; Malzacher, P.; Mamonov, A.; Manceau, L.; Manko, V.; Manso, F.; Manzari, V.; Marchisone, M.; Mareš, J.; Margagliotti, G. V.; Margotti, A.; Margutti, J.; Marín, A.; Markert, C.; Marquard, M.; Martin, N. A.; Martin Blanco, J.; Martinengo, P.; Martínez, M. I.; Martínez García, G.; Martinez Pedreira, M.; Martynov, Y.; Mas, A.; Masciocchi, S.; Masera, M.; Masoni, A.; Massacrier, L.; Mastroserio, A.; Masui, H.; Matyja, A.; Mayer, C.; Mazer, J.; Mazzoni, M. A.; Mcdonald, D.; Meddi, F.; Menchaca-Rocha, A.; Meninno, E.; Mercado Pérez, J.; Meres, M.; Miake, Y.; Mieskolainen, M. M.; Mikhaylov, K.; Milano, L.; Milosevic, J.; Minervini, L. M.; Mischke, A.; Mishra, A. N.; Miskowiec, D.; Mitra, J.; Mitu, C. M.; Mohammadi, N.; Mohanty, B.; Molnar, L.; Montaño Zetina, L.; Montes, E.; Morando, M.; Moreira De Godoy, D. A.; Moretto, S.; Morreale, A.; Morsch, A.; Muccifora, V.; Mudnic, E.; Mühlheim, D.; Muhuri, S.; Mukherjee, M.; Müller, H.; Mulligan, J. D.; Munhoz, M. G.; Murray, S.; Musa, L.; Musinsky, J.; Nandi, B. K.; Nania, R.; Nappi, E.; Naru, M. U.; Nattrass, C.; Nayak, K.; Nayak, T. K.; Nazarenko, S.; Nedosekin, A.; Nellen, L.; Ng, F.; Nicassio, M.; Niculescu, M.; Niedziela, J.; Nielsen, B. S.; Nikolaev, S.; Nikulin, S.; Nikulin, V.; Noferini, F.; Nomokonov, P.; Nooren, G.; Norman, J.; Nyanin, A.; Nystrand, J.; Oeschler, H.; Oh, S.; Oh, S. K.; Ohlson, A.; Okatan, A.; Okubo, T.; Olah, L.; Oleniacz, J.; Oliveira Da Silva, A. C.; Oliver, M. H.; Onderwaater, J.; Oppedisano, C.; Ortiz Velasquez, A.; Oskarsson, A.; Otwinowski, J.; Oyama, K.; Ozdemir, M.; Pachmayer, Y.; Pagano, P.; Paić, G.; Pajares, C.; Pal, S. K.; Pan, J.; Pandey, A. K.; Pant, D.; Papikyan, V.; Pappalardo, G. S.; Pareek, P.; Park, W. J.; Parmar, S.; Passfeld, A.; Paticchio, V.; Patra, R. N.; Paul, B.; Peitzmann, T.; Pereira Da Costa, H.; Pereira De Oliveira Filho, E.; Peresunko, D.; Pérez Lara, C. E.; Peskov, V.; Pestov, Y.; Petráček, V.; Petrov, V.; Petrovici, M.; Petta, C.; Piano, S.; Pikna, M.; Pillot, P.; Pinazza, O.; Pinsky, L.; Piyarathna, D. B.; Ploskon, M.; Planinic, M.; Pluta, J.; Pochybova, S.; Podesta-Lerma, P. L. M.; Poghosyan, M. G.; Polichtchouk, B.; Poljak, N.; Poonsawat, W.; Pop, A.; Porteboeuf-Houssais, S.; Porter, J.; Pospisil, J.; Prasad, S. K.; Preghenella, R.; Prino, F.; Pruneau, C. A.; Pshenichnov, I.; Puccio, M.; Puddu, G.; Pujahari, P.; Punin, V.; Putschke, J.; Qvigstad, H.; Rachevski, A.; Raha, S.; Rajput, S.; Rak, J.; Rakotozafindrabe, A.; Ramello, L.; Raniwala, R.; Raniwala, S.; Räsänen, S. S.; Rascanu, B. T.; Rathee, D.; Read, K. F.; Real, J. S.; Redlich, K.; Reed, R. J.; Rehman, A.; Reichelt, P.; Reidt, F.; Ren, X.; Renfordt, R.; Reolon, A. R.; Reshetin, A.; Rettig, F.; Revol, J.-P.; Reygers, K.; Riabov, V.; Ricci, R. A.; Richert, T.; Richter, M.; Riedler, P.; Riegler, W.; Riggi, F.; Ristea, C.; Rivetti, A.; Rocco, E.; Rodríguez Cahuantzi, M.; Rodriguez Manso, A.; Røed, K.; Rogochaya, E.; Rohr, D.; Röhrich, D.; Romita, R.; Ronchetti, F.; Ronflette, L.; Rosnet, P.; Rossi, A.; Roukoutakis, F.; Roy, A.; Roy, C.; Roy, P.; Rubio Montero, A. J.; Rui, R.; Russo, R.; Ryabinkin, E.; Ryabov, Y.; Rybicki, A.; Sadovsky, S.; Šafařík, K.; Sahlmuller, B.; Sahoo, P.; Sahoo, R.; Sahoo, S.; Sahu, P. K.; Saini, J.; Sakai, S.; Saleh, M. A.; Salgado, C. A.; Salzwedel, J.; Sambyal, S.; Samsonov, V.; Sanchez Castro, X.; Šándor, L.; Sandoval, A.; Sano, M.; Santagati, G.; Sarkar, D.; Scapparone, E.; Scarlassara, F.; Scharenberg, R. P.; Schiaua, C.; Schicker, R.; Schmidt, C.; Schmidt, H. R.; Schuchmann, S.; Schukraft, J.; Schulc, M.; Schuster, T.; Schutz, Y.; Schwarz, K.; Schweda, K.; Scioli, G.; Scomparin, E.; Scott, R.; Seeder, K. S.; Seger, J. E.; Sekiguchi, Y.; Selyuzhenkov, I.; Senosi, K.; Seo, J.; Serradilla, E.; Sevcenco, A.; Shabanov, A.; Shabetai, A.; Shadura, O.; Shahoyan, R.; Shangaraev, A.; Sharma, A.; Sharma, N.; Shigaki, K.; Shtejer, K.; Sibiriak, Y.; Siddhanta, S.; Sielewicz, K. M.; Siemiarczuk, T.; Silvermyr, D.; Silvestre, C.; Simatovic, G.; Simonetti, G.; Singaraju, R.; Singh, R.; Singha, S.; Singhal, V.; Sinha, B. C.; Sinha, T.; Sitar, B.; Sitta, M.; Skaali, T. B.; Skjerdal, K.; Slupecki, M.; Smirnov, N.; Snellings, R. J. M.; Snellman, T. W.; Søgaard, C.; Soltz, R.; Song, J.; Song, M.; Song, Z.; Soramel, F.; Sorensen, S.; Spacek, M.; Spiriti, E.; Sputowska, I.; Spyropoulou-Stassinaki, M.; Srivastava, B. K.; Stachel, J.; Stan, I.; Stefanek, G.; Steinpreis, M.; Stenlund, E.; Steyn, G.; Stiller, J. H.; Stocco, D.; Strmen, P.; Suaide, A. A. P.; Sugitate, T.; Suire, C.; Suleymanov, M.; Sultanov, R.; Šumbera, M.; Symons, T. J. M.; Szabo, A.; Szanto de Toledo, A.; Szarka, I.; Szczepankiewicz, A.; Szymanski, M.; Takahashi, J.; Tanaka, N.; Tangaro, M. A.; Tapia Takaki, J. D.; Tarantola Peloni, A.; Tariq, M.; Tarzila, M. G.; Tauro, A.; Tejeda Muñoz, G.; Telesca, A.; Terasaki, K.; Terrevoli, C.; Teyssier, B.; Thäder, J.; Thomas, D.; Tieulent, R.; Timmins, A. R.; Toia, A.; Trogolo, S.; Trubnikov, V.; Trzaska, W. H.; Tsuji, T.; Tumkin, A.; Turrisi, R.; Tveter, T. S.; Ullaland, K.; Uras, A.; Usai, G. L.; Utrobicic, A.; Vajzer, M.; Vala, M.; Valencia Palomo, L.; Vallero, S.; Van Der Maarel, J.; Van Hoorne, J. W.; van Leeuwen, M.; Vanat, T.; Vande Vyvre, P.; Varga, D.; Vargas, A.; Vargyas, M.; Varma, R.; Vasileiou, M.; Vasiliev, A.; Vauthier, A.; Vechernin, V.; Veen, A. M.; Veldhoen, M.; Velure, A.; Venaruzzo, M.; Vercellin, E.; Vergara Limón, S.; Vernet, R.; Verweij, M.; Vickovic, L.; Viesti, G.; Viinikainen, J.; Vilakazi, Z.; Villalobos Baillie, O.; Vinogradov, A.; Vinogradov, L.; Vinogradov, Y.; Virgili, T.; Vislavicius, V.; Viyogi, Y. P.; Vodopyanov, A.; Völkl, M. A.; Voloshin, K.; Voloshin, S. A.; Volpe, G.; von Haller, B.; Vorobyev, I.; Vranic, D.; Vrláková, J.; Vulpescu, B.; Vyushin, A.; Wagner, B.; Wagner, J.; Wang, H.; Wang, M.; Wang, Y.; Watanabe, D.; Weber, M.; Weber, S. G.; Wessels, J. P.; Westerhoff, U.; Wiechula, J.; Wikne, J.; Wilde, M.; Wilk, G.; Wilkinson, J.; Williams, M. C. S.; Windelband, B.; Winn, M.; Yaldo, C. G.; Yamaguchi, Y.; Yang, H.; Yang, P.; Yano, S.; Yin, Z.; Yokoyama, H.; Yoo, I.-K.; Yurchenko, V.; Yushmanov, I.; Zaborowska, A.; Zaccolo, V.; Zaman, A.; Zampolli, C.; Zanoli, H. J. C.; Zaporozhets, S.; Zarochentsev, A.; Závada, P.; Zaviyalov, N.; Zbroszczyk, H.; Zgura, I. S.; Zhalov, M.; Zhang, H.; Zhang, X.; Zhang, Y.; Zhao, C.; Zhigareva, N.; Zhou, D.; Zhou, Y.; Zhou, Z.; Zhu, H.; Zhu, J.; Zhu, X.; Zichichi, A.; Zimmermann, A.; Zimmermann, M. B.; Zinovjev, G.; Zyzak, M.

    2015-09-01

    We report the first measurement at the LHC of coherent photoproduction of ρ 0 mesons in ultra-peripheral Pb-Pb collisions. The invariant mass and transverse momentum distributions for ρ 0 production are studied in the π + π - decay channel at mid-rapidity. The production cross section in the rapidity range | y| < 0.5 is found to be d σ/d y = 425 ± 10(stat.) - 50 + 42 (sys.) mb. Coherent ρ 0 production is studied with and without requirement of nuclear breakup, and the fractional yields for various breakup scenarios are presented. The results are compared with those from lower energies and with model predictions. [Figure not available: see fulltext.

  11. Cytotoxicity on Allium cepa of the two main sulcotrione photoproducts, xanthene-1,9-dione-3,4-dihydro-6-methylsulphonyl and 2-chloro-4-mesylbenzoic acid.

    PubMed

    Goujon, Eric; Richard, Claire; Goupil, Pascale; Ledoigt, Gérard

    2015-10-01

    The cytotoxic effects of 2-chloro-4-mesylbenzoic acid (CMBA) and xanthene-1,9-dione-3,4-dihydro-6-methylsulphonyl (XDD), the two main photoproducts of sulcotrione, were investigated on Allium root meristematic cells at different concentrations. Degradation of sulcotrione was correlated to mitotic index decrease, together with increasing anomaly and c-mitosis frequencies. Mitotic index significantly decreased with increasing XDD and CMBA concentrations. Cell frequency with abnormal chromosomes increased with CMBA or XDD application rates. In contrast, CMBA induced a low micronucleus rate even for high concentrations while XDD increased the micronucleus ratio. C-mitoses, chromosomal aberrations due to an inactivation of the spindle, were enhanced by CMBA treatments but not by XDD. The photochemical degradation process of the pesticide can change the risk for the environment. PMID:26453228

  12. Light Vector Meson Photoproduction off of 1H at Jefferson Lab and p-w Interference in the Leptonic Decay Channel

    SciTech Connect

    Djalali, Chaden; Paolone, Michael; Weygand, Dennis; Wood, Mike H.

    2014-09-01

    Although the phenomena of r – w interference has been studied at great length in pionic decay channel over the past 50 years, a study of the interference in a purely electromagnetic production and decay channel has never been performed on an elementary proton target until now. The only published photo-production data of the r - w leptonic decay channel was obtained in the early seventies on C and Be. An investigation of the r - w interference on a Hydrogen was recently completed at Jefferson Lab with the CLAS detector. The di-lepton spectra was fit with two inter- fering relativistic Breit-Wigner functions, and the interference phase was extracted. Preliminary results will be compared to the previous experimental studies in nuclei.

  13. Photoproduction of vector mesons in ultra-peripheral p–Pb and Pb–Pb collisions at the LHC with the ALICE experiment

    SciTech Connect

    Broz, Michal

    2015-04-10

    Vector mesons are copiously produced in ultra-peripheral collisions. In these collisions, the impact parameter is larger than the sum of the radii of the two projectiles, implying that electromagnetic processes become dominant. The cross section of production of vector mesons is expected to be sensitive to the gluon distribution and can therefore probe nuclear gluon shadowing (Pb–Pb) and the gluon structure function in the nucleon (p-Pb). The ALICE Collaboration has performed the first measurements of the production of ρ{sup 0}, J/ψ and ψ/(2S) in Pb–Pb ultra-peripheral collision as well as the cross section for exclusive J/ψ photoproduction off protons in ultra-peripheral proton-lead collisions at the LHC. The results are compared to the STARLIGHT Monte Carlo and to QCD based models.

  14. On theoretical uncertainty of color dipole phenomenology in the J/\\psi and ϒ photoproduction in pA and AA collisions at the CERN Large Hadron Collider

    NASA Astrophysics Data System (ADS)

    Sampaio dos Santos, G.; Machado, M. V. T.

    2015-10-01

    We investigate the theoretical uncertainty of the predictions for the photoproduction of J/\\psi and ϒ states in proton-nucleus and nucleus-nucleus collisions at the Large Hadron Collider (LHC) within the color dipole formalism. Predictions for the rapidity distributions are presented and the dependence on the meson wavefunction, heavy quark mass as well as the models for the dipole cross section are analyzed. We compare the theoretical results directly with recent data from the ALICE Collaboration on J/\\psi production in pPb collisions at a centre-of-mass energy per nucleon pair of 5.02 TeV and in PbPb collisions at a centre-of-mass energy per nucleon pair of 2.76 TeV. Predictions are also performed for ϒ state in PbPb and pPb collisions at LHC energies, including the coherent and incoherent contributions.

  15. A novel lyotropic liquid crystal formed by triphilic star-polyphiles: hydrophilic/oleophilic/fluorophilic rods arranged in a 12.6.4. tiling.

    PubMed

    de Campo, Liliana; Varslot, Trond; Moghaddam, Minoo J; Kirkensgaard, Jacob J K; Mortensen, Kell; Hyde, Stephen T

    2011-02-28

    Triphilic star-polyphiles are short-chain oligomeric molecules with a radial arrangement of hydrophilic, hydrocarbon and fluorocarbon chains linked to a common centre. They form a number of liquid crystalline structures when mixed with water. In this contribution we focus on a hexagonal liquid crystalline mesophase found in star-polyphiles as compared to the corresponding double-chain surfactant to determine whether the hydrocarbon and fluorocarbon chains are in fact demixed in these star-polyphile systems, or whether both hydrocarbon and fluorocarbon chains are miscible, leading to a single hydrophobic domain, making the star-polyphile effectively amphiphilic. We report SANS contrast variation data that are compatible only with the presence of three distinct immiscible domains within this hexagonal mesophase, confirming that these star-polyphile liquid crystals are indeed hydrophilic/oleophilic/fluorophilic 3-phase systems. Quantitative comparison with scattering simulations shows that the experimental data are in very good agreement with an underlying 2D columnar (12.6.4) tiling. As in a conventional amphiphilic hexagonal mesophase, the hexagonally packed water channels (dodecagonal prismatic domains) are embedded in a hydrophobic matrix, but that matrix is split into oleophilic hexagonal prismatic domains and fluorophilic quadrangular prismatic domains.

  16. Surface deformation and subsurface slip of the 28 March 1999 Mw = 6.4 west Himalayan Chamoli earthquake from InSAR analysis

    NASA Astrophysics Data System (ADS)

    Satyabala, S. P.; Bilham, Roger

    2006-12-01

    We report InSAR observations of coseismic ground deformation caused by the Mw6.4 Chamoli earthquake of March 28, 1999 in the western Himalaya near the surface trace of the Main Central Thrust. Analysis of ERS1/2-SAR data from ascending and descending tracks reveals surface deformation in a region 30 km by 40 km with a maximum coseismic uplift of ~60 mm. Assuming that the rupture occurred as a planar uniform slip dislocation in an elastic half space reveals slip of 0.55 +/- 0.1 m, strike of N300°E, 15° dip, with fault dimensions of 13 +/- 3 km along-strike and 10 +/- 3 km downdip. The corresponding moment magnitude is Mw = 6.2, lower than that derived from seismological methods. With the exception of the recent 2005 Kashmir earthquake, the Chamoli earthquake studied in this paper is the only Himalayan earthquake for which surface deformation data are available from directly above the epicenter.

  17. M -shell x-ray production by 0. 6--4. 0-MeV protons in ten elements from hafnium to thorium

    SciTech Connect

    Pajek, M. ); Kobzev, A.P.; Sandrik, R.; Skrypnik, A.V. ); Ilkhamov, R.A.; Khusmurodov, S.H. ); Lapicki, G. )

    1990-07-01

    {ital M}-shell x-ray production cross sections for selected heavy elements, namely, {sub 72}Hf, {sub 73}Ta, {sub 74}W, {sub 75}Re, {sub 76}Os, {sub 77}Ir, {sub 78}Pt, {sub 79}Au, {sub 83}Bi, and {sub 90}Th, were measured for protons of energy 0.6--4.0 MeV. The experimental results are compared with the predictions of the first Born and semiclassical approximations for {ital M}-shell ionization; these data are also compared with the theory that accounts for the projectile's energy loss and Coulomb deflection as well as for the target's {ital M}-shell electron perturbed stationary state and relativistic nature (ECPSSR). Generally, fair agreement between the data and the ECPSSR theory is found. Some systematical discrepancies observed for the lightest elements (Hf, Ta, and W) are explained as possible ambiguities in the {ital M}-shell Coster-Kronig factors and fluorescence yields, which were used to convert theoretical {ital M}-subshell ionization cross sections to the total {ital M}-x-ray production cross sections. The experimental total {ital M}-shell ionization cross sections were obtained from measured {ital M}-x-ray cross sections using the proposed approximate average fluorescence yield {bar {omega}}{sub {ital M}} that relies on two fluorescence yields and the Coster-Kronig factor for {ital only} {ital M}{sub 4} and {ital M}{sub 5} subshells.

  18. Polyamine Resistance Is Increased by Mutations in a Nitrate Transporter Gene NRT1.3 (AtNPF6.4) in Arabidopsis thaliana

    PubMed Central

    Tong, Wurina; Imai, Akihiro; Tabata, Ryo; Shigenobu, Shuji; Yamaguchi, Katsushi; Yamada, Masashi; Hasebe, Mitsuyasu; Sawa, Shinichiro; Motose, Hiroyasu; Takahashi, Taku

    2016-01-01

    Polyamines are small basic compounds present in all living organisms and act in a variety of biological processes. However, the mechanism of polyamine sensing, signaling and response in relation to other metabolic pathways remains to be fully addressed in plant cells. As one approach, we isolated Arabidopsis mutants that show increased resistance to spermine in terms of chlorosis. We show here that two of the mutants have a point mutation in a nitrate transporter gene of the NRT1/PTR family (NPF), NRT1.3 (AtNPF6.4). These mutants also exhibit increased resistance to putrescine and spermidine while loss-of-function mutants of the two closest homologs of NRT1.3, root-specific NRT1.1 (AtNPF6.3) and petiole-specific NRT1.4 (AtNPF6.2), were shown to have a normal sensitivity to polyamines. When the GUS reporter gene was expressed under the control of the NRT1.3 promoter, GUS staining was observed in leaf mesophyll cells and stem cortex cells but not in the epidermis, suggesting that NRT1.3 specifically functions in parenchymal tissues. We further found that the aerial part of the mutant seedling has normal levels of polyamines but shows reduced uptake of norspermidine compared with the wild type. These results suggest that polyamine transport or metabolism is associated with nitrate transport in the parenchymal tissue of the shoot. PMID:27379127

  19. Construction and 13C NMR signal-amplification efficiency of a dynamic nuclear polarizer at 6.4 T and 1.4 K

    NASA Astrophysics Data System (ADS)

    Kiswandhi, Andhika; Niedbalski, Peter; Parish, Christopher; Ferguson, Sarah; Taylor, David; McDonald, George; Lumata, Lloyd

    Dissolution dynamic nuclear polarization (DNP) is a rapidly emerging technique in biomedical and metabolic imaging since it amplifies the liquid-state nuclear magnetic resonance (NMR) and imaging (MRI) signals by >10,000-fold. Originally used in nuclear scattering experiments, DNP works by creating a non-Boltzmann nuclear spin distribution by transferring the high electron (γ = 28,000 MHz/T) thermal polarization to the nuclear spins via microwave irradiation of the sample at high magnetic field and low temperature. A dissolution device is used to rapidly dissolve the frozen sample and consequently produces an injectable ``hyperpolarized'' liquid at physiologically-tolerable temperature. Here we report the construction and performance evaluation of a dissolution DNP hyperpolarizer at 6.4 T and 1.4 K using a continuous-flow cryostat. The solid and liquid-state 13C NMR signal enhancement levels of 13C acetate samples doped with trityl OX063 and 4-oxo-TEMPO free radicals will be discussed and compared with the results from the 3.35 T commercial hyperpolarizer. This work is supported by US Dept of Defense Award No. W81XWH-14-1-0048 and Robert A. Welch Foundation Grant No. AT-1877.

  20. Synthesis of polypyrrole within the cell wall of yeast by redox-cycling of [Fe(CN)6](3-)/[Fe(CN)6](4-).

    PubMed

    Ramanavicius, Arunas; Andriukonis, Eivydas; Stirke, Arunas; Mikoliunaite, Lina; Balevicius, Zigmas; Ramanaviciene, Almira

    2016-02-01

    Yeast cells are often used as a model system in various experiments. Moreover, due to their high metabolic activity, yeast cells have a potential to be applied as elements in the design of biofuel cells and biosensors. However a wider application of yeast cells in electrochemical systems is limited due to high electric resistance of their cell wall. In order to reduce this problem we have polymerized conducting polymer polypyrrole (Ppy) directly in the cell wall and/or within periplasmic membrane. In this research the formation of Ppy was induced by [Fe(CN)6](3-)ions, which were generated from K4[Fe(CN)6], which was initially added to polymerization solution. The redox process was catalyzed by oxido-reductases, which are present in the plasma membrane of yeast cells. The formation of Ppy was confirmed by spectrophotometry and atomic force microscopy. It was confirmed that the conducting polymer polypyrrole was formed within periplasmic space and/or within the cell wall of yeast cells, which were incubated in solution containing pyrrole, glucose and [Fe(CN)6](4-). After 24h drying at room temperature we have observed that Ppy-modified yeast cell walls retained their initial spherical form. In contrast to Ppy-modified cells, the walls of unmodified yeast have wrinkled after 24h drying. The viability of yeast cells in the presence of different pyrrole concentrations has been evaluated.

  1. Synthesis, molecular docking and biological evaluation of novel 6-(4-(4-aminophenylsulfonyl)phenylamino)-5H-benzo[a]phenothiazin-5-one derivatives

    NASA Astrophysics Data System (ADS)

    Ravichandiran, Palanisamy; Athinarayanan, Jegan; Premnath, Dhanaraj; Periasamy, Vaiyapuri Subbarayan; Alshatwi, Ali A.; Vasanthkumar, Samuel

    2015-03-01

    A novel series of 6-(4-(4-aminophenylsulfonyl)phenylamino)-5H-benzo[a]phenothiazin-5-one derivatives have been synthesized and examined for their in vitro antibacterial activity against a panel of Gram-positive and Gram-negative bacteria. Among these, N-(4-(4-(5-oxo-5H-benzo[a]phenothiazin-6-ylamino)phenylsulfonyl)phenyl)-3,5-bis(trifluoromethyl)benzamide (3n) (0.4 μg/mL) and 4-ethyl-N-(4-(4-(5-oxo-5H-benzo[a]phenothiazin-6-ylamino)phenylsulfonyl)phenyl)benzamide (3l) (0.6 μg/mL) systems exhibited a potent inhibitory activity against Gram-positive organism Bacillus subtilis, when compare to the other synthesized compounds. Sparfloxacin (9.76 μg/mL), Norfloxacin (no activity) were employed as the standard drugs. An evaluation of the cytotoxicity of the title compounds (1, 2, 3a-n) revealed that they displayed low toxicity (26-115 mg/L) against cervical cancer cell line (SiHa). The results of these studies suggest that, phenothiazin-5-one derivatives are interesting binding agents for the development of new Gram-positive and Gram-negative antibacterial agents. To understand the interactions with protein receptors, docking simulation was done with crystal structures of B.subtilis (YmaH) and histone deacetylase (HDAC8) to determine the probable binding conformation.

  2. Adipic acid–2,4-diamino-6-(4-meth­oxy­phen­yl)-1,3,5-triazine (1/2)

    PubMed Central

    Thanigaimani, Kaliyaperumal; Razak, Ibrahim Abdul; Arshad, Suhana; Jagatheesan, Rathinavel; Santhanaraj, Kulandaisamy Joseph

    2012-01-01

    The asymmetric unit of the title compound, 2C10H11N5O·C6H10O4, consists of a 2,4-diamino-6-(4-meth­oxy­phen­yl)-1,3,5-triazine mol­ecule and one-half mol­ecule of adipic acid which lies about an inversion center. The triazine ring makes a dihedral angle of 12.89 (4)° with the adjacent benzene ring. In the crystal, the components are linked by N—H⋯O and O—H⋯N hydrogen bonds, thus generating a centrosymmetric 2 + 1 unit of triazine and adipic acid mol­ecules with R 2 2(8) motifs. The triazine mol­ecules are connected to each other by N—H⋯N hydrogen bonds, forming an R 2 2(8) motif and a supra­molecular ribbon along the c axis. The 2 + 1 units and the supra­molecular ribbons are further inter­linked by weak N—H⋯O, C—H⋯O and C—H⋯π inter­actions, resulting in a three-dimensional network. PMID:23125724

  3. Super-soft X-ray emission on day 6.4 from nova LMC N1968-12a strongly suggests a very high mass white dwarf

    NASA Astrophysics Data System (ADS)

    Page, K. L.; Osborne, J. P.; Darnley, M. J.; Henze, M.; Kuin, N. P. M.; Schwarz, G. J.; Shore, S. N.; Starrfield, S.; Williams, S. C.

    2016-01-01

    Swift observations of the 2016 outburst of LMCN 1968-12a (ATel #8578, #8586, #8587) have been continuing daily. The early XRT spectrum, covering 2016 Jan 23 - 25 (days 2.07-4.26 after outburst), can be modelled by a single temperature optically thin emission component of kT & gt; 0.9 keV and NH = (8.3 +21.7/-7.8)x1021 cm-2, with a mean 0.3-10 keV observed flux of (5.1 +8.2/-2.3)x10-13 erg cm-2 s-1. After this time, an increase in soft emission (counts below ~1 keV) was seen, although at first this appeared to be related to a decrease in the absorbing column; no corresponding increase in X-ray count rate was found, remaining around 0.016 count s-1. On 2016 Jan 27 (day 6.4), the X-ray count rate rose to 0.05 count s-1 with the emission being dominated by counts below 1 keV. We identify this as the start of the super-soft source (SSS) phase.

  4. Competition of nucleoside transport inhibitors with binding of 6-[(4-nitrobenzyl)-mercapto]purine ribonucleoside to intact erythrocytes and ghost membranes from different species.

    PubMed

    Ogbunude, P O; Baer, H P

    1990-04-01

    The potency of nucleoside transport inhibitors, including 6-[(4-nitrobenzyl)-mercapto]purine ribonucleoside (NBMPR), dilazep, mioflazine and its derivatives soluflazine and R57974 as inhibitors of the binding of [3H(G)]NBMPR to intact erythrocytes and respective ghost membranes from human, mouse and hamster was determined. There was no close agreement between the IC50 profiles for the different inhibitors when comparing values obtained for intact cells and membranes from each species, and there was no consistent profile of differences when considering individual drugs and comparing their actions in the three species. Present data also were compared with potency values obtained previously with the same drugs directly in nucleoside transport inhibition studies with erythrocytes from the same species as well as with [3H(G)]NBMPR binding studies in isolated liver and lung membranes from hamster. The overall conclusion from this and previous studies is that the evaluation of relative potencies in screening of potential nucleoside transport inhibitors is best carried out at the level actual nucleoside transport studies in intact cells, since [3H(G)]NBMPR binding studies yield discrepant data. PMID:2322305

  5. FRACTURE TOUGHNESS OF 6.4 MM (0.25 INCH) ARC-CAST MOLOBDENUM AND MOLYBDENUM-TZM PLATE AT ROOM TEMPERATURE AND 300 DEGREES C

    SciTech Connect

    J. A. SHIELDS, JR.; P. LIPETZKY; A. J. MUELLER

    2001-04-11

    THE FRACTURE TOUGHNESS OF 6.4 mm (0.25 INCH) LOW CARBON ARC-CAST (LCAC) MOLYBDENUM AND ARC-CAST MOLYBDENUM-TZM ALLOY PLATE WERE MEASURED AT ROOM TEMPERATURE AND 300 DEGREES C USING COMPACT TNESION SPECIMENTS. THE EFFECT OF CRACK PLANE ORIENTATION (LONGITUDINAL VS. TRANSVERSE) AND ANNEALING PRACTICE (STRESS-RELIEVED VS. RECRYSTALLIZED) WERE EVALUATED. DEPENDING UPON THE TEST TEMPERATURE EITHER A STANDARD K[SUB]IC OR A J-INTEGRAL ANALYSIS WAS USED TO OBTAIN THE TOUGHNESS VALUE. AT ROOM TEMPERATURE, REGARDLESS OF ALLOY, ORIENTATION, OR MICROSTURECTURE, FRACTURE TOUGHNESS VALUES BETWEEN 15 AND 22 MPa m{sup 1/2} (14 AND 20 KSI IN{sup 1/2}) WERE MEASURED. THESE K[SUB]IC VALUES WERE CONSISTENT WITH MEASUREMENTS BY THE AUTHORS. INCREASING TEMPERATURE IMPROVES THE TOUGHNESS, DUE TO THE FACT THAT ONE TAKES ADVANTAGE OF THE DUCTIVE-BRITTLE TRANSITION BEHAVIOR OF MOLYBDENUM. AT 300 DEGREES C, THE FRACTURE TOUGHNESS OF RECRYSTALLIZED LCAC AND ARC-CAST TZM MOLYBDENUM WERE ALSO SIMILAR AT APPROXI MATELY 64 MPa m{sup 1/2} (58 KSI IN{sup 1/2}). IN THE STRESS-RELIEVED CONDITION, HOWEVER, THE TOUGHNESS OF ARC-CAST TZM (91 MPa m{sup 1/2}/83 KSI IN{sup 1/2}) WAS HIGHER THAN THAT OF THE LCAC MOLYBDENUM (74 MPa m{sup 1/2}/67 KSI IN{sup 1/2}).

  6. Two saturable recognition sites for (-) (125I)iodo-N6-(4-hydroxyphenyl-isopropyl)-adenosine binding on purified cardiac sarcolemma

    SciTech Connect

    Hausleithner, V.; Freissmuth, M.; Schuetz, W.

    1986-01-01

    Analysis of (-) (125)iodo-N6-(4-hydroxyphenylisopropyl)-adenosine (( /sup 125/I)HPIA) binding to purified sarcolemmal preparations of guinea pig and bovine hearts revealed two classes of binding sites when unlabeled iodo-HPIA (100 mumol/l) was used as non-specific binding marker. In the presence of 1 mmol/l theophylline, however, only the high affinity component was detected. Adenosine receptor agonists caused biphasic displacement of (/sup 125/I)HPIA binding, with a high affinity potency rank order typical of interaction with A1-adenosine receptors. Biphasic competition curves were also observed with 8-phenyltheophylline and isobutylmethylxanthine, whereas the theophylline curve was monophasic up to 1 mmol/l. In brain membranes, specific binding of (/sup 125/I)HPIA as well as of (/sup 3/H)PIA was further reduced when unlabeled iodo-HPIA replaces theophylline as the non-specific binding marker. These results suggest the presence of two (/sup 125/I)HPIA binding sites on cardiac sarcolemma and brain membranes, but receptor function can only be ascribed to the high affinity sites. The low affinity site probably represents an artefact, which is often observed when non-specific binding is defined with the unlabeled counterpart or a structurally related ligand of the radioligand used.

  7. Monohydroxylated Polybrominated Diphenyl Ethers (OH-PBDEs) and Dihydroxylated Polybrominated Biphenyls (Di-OH-PBBs): Novel Photoproducts of 2,6-Dibromophenol.

    PubMed

    Zhao, Hongxia; Jiang, Jingqiu; Wang, Yanli; Lehmler, Hans-Joachim; Buettner, Garry R; Quan, Xie; Chen, Jingwen

    2015-12-15

    Hydroxylated polybromodiphenyl ethers (OH-PBDEs) are emerging aquatic pollutants, but their origins in the environment are not fully understood. There is evidence that OH-PBDEs are formed from bromophenols, but the underlying transformation processes remain unknown. Here, we investigate if the photoformation of OH-PBDEs from 2,6-dibromophenol in aqueous solution involves 2,6-bromophenoxyl radicals. After the UV irradiation of an aqueous 2,6-dibromophenol solution, HPLC-LTQ-Orbitrap MS and GC-MS analysis revealed the formation of a OH-PBDE and a dihydroxylated polybrominated biphenyl (di-OH-PBB). Both dimeric photoproducts were tentatively identified as 4'-OH-BDE73 and 4,4'-di-OH-PBB80. In addition, three debromination products (4-OH-BDE34, 4'-OH-BDE27, and 4,4'-di-OH-PBBs) were observed. Electron paramagnetic resonance spectroscopy revealed the presence of a 2,6-dibromophenoxyl radical with a six-line spectrum (a(H) (2 meta) = 3.45 G, a(H) (1 para) = 1.04 G, g = 2.0046) during irradiation of a 2,6-dibromophenol solution in water. The 2,6-dibromophenoxyl radical had a relatively long half-life (122 ± 5 μs) according to laser flash photolysis experiments. The para-para C-C and O-para-C couplings of these 2,6-dibromophenoxyl radicals are consistent with the observed formation of both dimeric OH-PBDE and di-OH-PBB photoproducts. These findings show that bromophenoxyl radical-mediated phototransformation of bromophenols is a source of OH-PBDEs and di-OH-PBBs in aqueous environments that requires further attention. PMID:26545041

  8. Toxicity of a PAH photooxidation product to the bacteria Photobacterium phosphoreum and the duckweed Lemna gibba: Effects of phenanthrene and its primary photoproduct, phenanthrenequinone

    SciTech Connect

    McConkey, B.J.; Duxbury, C.L.; Dixon, D.G.; Greenberg, B.M.

    1997-05-01

    Phenanthrene (PHE) undergoes a significant increase in toxicity after exposure to simulated or natural sunlight in aqueous media, coincident with the appearance of PHE photoproducts. To investigate whether the primary photoproduct of PHE, 9,10-phenanthrenequinone (PHEQ), contributes to the increased hazards of solutions containing photomodified PHE, toxicity assays were conducted using the marine bacteria Photobacterium phosphoreum and the aquatic plant Lemna gibba (duckweed). Photobacterium phosphoreum was exposed to PHE, PHEQ, a photomodified PHE mixture containing known amounts of PHE and PHEQ (pmPHE), and a mixture mimicking the amounts of PHE and PHEQ in the pmPHE mixture. The bacteria were found to be equally sensitive to PHE in simulated solar radiation or darkness, with an EC50 of 0.53 mg/L. In both darkness or SSR, solutions containing PHEQ (with or without PHE) all exhibited an EC50 of 0.06 to 0.10 mg/L based on PHEQ concentrations, indicating that PHEQ was the primary active component of the pmPHE mixture. Lemna gibba was tested in SSR and visible light with PHE, PHEQ, and the pmPHE mixture. The calculated EC50 for PHE was 3.5 mg/L in SSR and 10.8 mg/L in visible light, showing that the presence of UV radiation in the SSR source increased the phytotoxicity of PHE. Strikingly, PHEQ was much more toxic to L. gibba than PHE in a light-independent manner. Thus, for both P. phosphoreum and L. gibba the major photooxidation product of PHE in SSR, PHEQ, is the more toxic of the two chemicals.

  9. Photoproduction of catalase-insensitive peroxides on the donor side of manganese-depleted photosystem II: evidence with a specific fluorescent probe.

    PubMed

    Khorobrykh, Sergey A; Khorobrykh, Andrei A; Yanykin, Denis V; Ivanov, Boris N; Klimov, Vyacheslav V; Mano, Jun'ichi

    2011-12-13

    The photoproduction of organic peroxides (ROOH) in photosystem II (PSII) membranes was studied using the fluorescent probe Spy-HP. Two types of peroxide, highly lipophilic ones and relatively hydrophilic ones, were distinguished by the rate of reaction with Spy-HP; the former oxidized Spy-HP to the higher fluorescent form Spy-HPOx within 5 min, while the latter did so very slowly (the reaction was still not completed after 180 min). The level of photoproduction of these peroxides was significantly larger in the alkaline-treated, Mn-depleted PSII membranes than that in the untreated membranes, and it was suppressed by an artificial electron donor (diphenylcarbazide or ferrocyanide) and by the electron transport inhibitor diuron. Postillumination addition of Fe(2+) ions, which degrade peroxides by the Fenton mechanism, abolished the accumulation of Spy-HPOx, but catalase did not change the peroxide level, indicating that the detected species were organic peroxides, excluding H(2)O(2). These results agreed with our previous observation of an electron transport-dependent O(2) consumption on the PSII donor side and indicated that ROOH accumulated via a radical chain reaction that started with the formation of organic radicals on the donor side. Illumination (λ > 600 nm; 1500 μmol of photons m(-2) s(-1)) of the Mn-depleted PSII membranes for 3 min resulted in the formation of nearly 200 molecules of hydrophilic ROOH per reaction center, but only four molecules of highly lipophilic ROOH. The limited formation of the latter was due to the limited supply of its precursor to the reaction, suggesting that it represented structurally fixed peroxides, i.e., either protein peroxides or peroxides of the lipids tightly bound to the core complex. These ROOH forms, likely including several species derived from lipid peroxides, may mediate the donor side-induced photoinhibition of PSII via protein modification.

  10. Plastidial Expression of Type II NAD(P)H Dehydrogenase Increases the Reducing State of Plastoquinones and Hydrogen Photoproduction Rate by the Indirect Pathway in Chlamydomonas reinhardtii1.

    PubMed

    Baltz, Anthony; Dang, Kieu-Van; Beyly, Audrey; Auroy, Pascaline; Richaud, Pierre; Cournac, Laurent; Peltier, Gilles

    2014-05-12

    Biological conversion of solar energy into hydrogen is naturally realized by some microalgae species due to a coupling between the photosynthetic electron transport chain and a plastidial hydrogenase. While promising for the production of clean and sustainable hydrogen, this process requires improvement to be economically viable. Two pathways, called direct and indirect photoproduction, lead to sustained hydrogen production in sulfur-deprived Chlamydomonas reinhardtii cultures. The indirect pathway allows an efficient time-based separation of O2 and H2 production, thus overcoming the O2 sensitivity of the hydrogenase, but its activity is low. With the aim of identifying the limiting step of hydrogen production, we succeeded in overexpressing the plastidial type II NAD(P)H dehydrogenase (NDA2). We report that transplastomic strains overexpressing NDA2 show an increased activity of nonphotochemical reduction of plastoquinones (PQs). While hydrogen production by the direct pathway, involving the linear electron flow from photosystem II to photosystem I, was not affected by NDA2 overexpression, the rate of hydrogen production by the indirect pathway was increased in conditions, such as nutrient limitation, where soluble electron donors are not limiting. An increased intracellular starch was observed in response to nutrient deprivation in strains overexpressing NDA2. It is concluded that activity of the indirect pathway is limited by the nonphotochemical reduction of PQs, either by the pool size of soluble electron donors or by the PQ-reducing activity of NDA2 in nutrient-limited conditions. We discuss these data in relation to limitations and biotechnological improvement of hydrogen photoproduction in microalgae. PMID:24820024

  11. The NR4A2 Nuclear Receptor Is Recruited to Novel Nuclear Foci in Response to UV Irradiation and Participates in Nucleotide Excision Repair

    PubMed Central

    Harrison, Matthew; Lim, Wen; Muscat, George E. O.; Sturm, Richard A.; Smith, Aaron G.

    2013-01-01

    Ultraviolet radiation (UVR) is one of the most common mutagens encountered by humans and induces the formation of cyclobutane pyrimidine dimers (CPDs) and pyrimidine-(6-4)-pyrimidone photoproduct (6-4PP) lesions in the genomic DNA. To prevent the accumulation of deleterious mutations these lesions must be efficiently repaired, primarily by nucleotide excision repair. We have previously demonstrated that the NR4A family of nuclear receptors are crucial mediators of the DNA repair function of the MC1R signalling pathway in melanocytes. Here we explore the role of the NR4A2 protein in the DNA repair process further. Using EYFP tagged-NR4A2 we have demonstrated a UVR induced recruitment to distinct nuclear foci where they co-localise with known DNA repair proteins. We reveal that the N-terminal domain of the receptor is required for this translocation and identify a role for p38 and PARP signalling in this process. Moreover disruption of the functional integrity of the Ligand Binding Domain of the receptor by deleting the terminal helix 12 effectively blocks co-localisation of the receptor with DNA repair factors. Restored co-localisation of the mutant receptor with DNA repair proteins in the presence of a Histone Deacetylase Inhibitor suggests that impaired chromatin accessibility underpins the mis-localisation observed. Finally NR4A2 over-expression facilitated a more efficient clearance of UVR induced CPD and 6-4PP lesions. Taken together these data uncover a novel role for the NR4A nuclear receptors as direct facilitators of nucleotide excision repair. PMID:24223135

  12. Faster DNA Repair of Ultraviolet-Induced Cyclobutane Pyrimidine Dimers and Lower Sensitivity to Apoptosis in Human Corneal Epithelial Cells than in Epidermal Keratinocytes

    PubMed Central

    Mallet, Justin D.; Bastien, Nathalie; Gendron, Sébastien P.; Rochette, Patrick J.

    2016-01-01

    Absorption of UV rays by DNA generates the formation of mutagenic cyclobutane pyrimidine dimers (CPD) and pyrimidine (6–4) pyrimidone photoproducts (6-4PP). These damages are the major cause of skin cancer because in turn, they can lead to signature UV mutations. The eye is exposed to UV light, but the cornea is orders of magnitude less prone to UV-induced cancer. In an attempt to shed light on this paradox, we compared cells of the corneal epithelium and the epidermis for UVB-induced DNA damage frequency, repair and cell death sensitivity. We found similar CPD levels but a 4-time faster UVB-induced CPD, but not 6-4PP, repair and lower UV-induced apoptosis sensitivity in corneal epithelial cells than epidermal. We then investigated levels of DDB2, a UV-induced DNA damage recognition protein mostly impacting CPD repair, XPC, essential for the repair of both CPD and 6-4PP and p53 a protein upstream of the genotoxic stress response. We found more DDB2, XPC and p53 in corneal epithelial cells than in epidermal cells. According to our results analyzing the protein stability of DDB2 and XPC, the higher level of DDB2 and XPC in corneal epithelial cells is most likely due to an increased stability of the protein. Taken together, our results show that corneal epithelial cells have a better efficiency to repair UV-induced mutagenic CPD. On the other hand, they are less prone to UV-induced apoptosis, which could be related to the fact that since the repair is more efficient in the HCEC, the need to eliminate highly damaged cells by apoptosis is reduced. PMID:27611318

  13. DD correlations in photoproduction

    NASA Astrophysics Data System (ADS)

    Alvarez, M. P.; Barate, R.; Bloch, D.; Bonamy, P.; Borgeaud, P.; Burchell, M.; Burmeister, H.; Brunet, J. M.; Calvino, F.; Cattaneo, M.; Crespo, J. M.; D'Almagne, B.; David, M.; di Ciaccio, L.; Dixon, J.; Druet, P.; Duane, A.; Engel, J. P.; Ferrer, A.; Filippas, T. A.; Fokitis, E.; Forty, R. W.; Foucault, P.; Gazis, E. N.; Gerber, J. P.; Giomataris, Y.; Hofmokl, T.; Katsoufis, E. C.; Koratzinos, M.; Krafft, C.; Lefievre, B.; Lemoigne, Y.; Lopez, A.; Lui, W. K.; Magneville, C.; Maltezos, A.; McEwen, J. G.; Papadopoulou, T.; Pattison, B.; Poutot, D.; Primout, M.; Rahmani, H.; Roudeau, P.; Seez, C.; Six, J.; Strub, R.; Treille, D.; Triscos, P.; Tristram, G.; Villet, G.; Volte, A.; Wayne, M.; Websdale, D. M.; Wormser, G.; Zolnierowski, Y.

    1992-03-01

    Kinematic correlations between the charmed D and D mesons produced by a photon beam of mean energy 100 GeV/c have been measured by the NA14/2 experiment at CERN using a sample of almost background-free fully reconstructed DD events. The observed D and DD distributions are compared to the predictions of production models using different parameters for the charm fragmentation function and for the intrinsic transverse momentum of the partons.

  14. Biological hydrogen photoproduction

    SciTech Connect

    Brand, L.E.; Luo, Yao-Hua; Tsuzuki, T.

    1996-10-01

    The authors maintain a culture collection of over 2,500 cyanobacteria and photosynthetic bacteria and 2,000 eukaryotic algae for research on H{sub 2} biophotoproduction as well as other projects. A high H{sub 2}-producing strain Synechococcus sp. Miami BG 043511 from the collection has been used to examine H{sub 2} production in relation to water photolysis and substance degradation, high O{sub 2}-tolerance characterization of nitrogenase, and interactive effects of light intensity and cell density on H{sub 2} production and its stable isotope fractionation. When cellular carbohydrate is depleted, no H{sub 2} production occurs. However, when pyruvate or glucose is added, H{sub 2} production begins. On the other hand, when glucose and DCMU are added to cellular carbohydrate-depleted cells from synchronous cultures, H{sub 2} production is inhibited compared with that with glucose addition alone. These results indicate that water photolysis alone cannot directly induce H{sub 2} production, but it acts together with cellular glucose degradation to enhance H{sub 2} production. The cyanobacteria strain, unlike unicellular green algae, shows no significant difference in H{sub 2} evolution with or without O{sub 2} removal reagent (Fieser`s solution: sodium dithionite/sodium anthraquinon-{beta}-sulfonate/KOH water solution) treated. When 10-hr cells are incubated in the dark with injected O{sub 2}, H{sub 2} evolution is induced, accompanied by O{sub 2} consumption. When zero-hr cells of synchronous cultures are incubated with 5% CO{sub 2}, the carbon is assimilated under light and O{sub 2} accumulates. Even when cells are under aerobic conditions with 25 {mu}mol O{sub 2}/Mg dry wt., H{sub 2} production is still induced, comparable with that under anaerobic conditions. The H{sub 2}-light curve has a pattern similar to CO{sub 2}-light curves, the light saturation point increasing, and the magnitude of H{sub 2} accumulation decreasing, with increasing cell density.

  15. Charged Pion Photoproduction

    SciTech Connect

    Haiyan Gao, Wei Chen

    2009-12-01

    We extracted the differential cross section for the gn --> pi-p process from a deuterium target using the CLAS detector at Jefferson Lab in Hall B for photon energies between 1.0 and 3.5 GeV and pion center-of-mass (c.m.) angles (theta c.m.) between 50 deg. and 115 deg. We confirm a previous indication of a broad enhancement around a c.m. energy (sqrt s) of 2.1 GeV at theta c.m. =90 deg. in the scaled differential cross section, s^7 ds/dt and a rapid fall-off in a center-of-mass energy region of about 400 MeV following the enhancement. Our data show an angular dependence of this enhancement as the suggested scaling region is approached for theta c.m. from 70 deg. to 10 deg.

  16. A new mineral species ferricoronadite, Pb[Mn6 4+(Fe3+, Mn3+)2]O16: mineralogical characterization, crystal chemistry and physical properties

    NASA Astrophysics Data System (ADS)

    Chukanov, Nikita V.; Aksenov, Sergey M.; Jančev, Simeon; Pekov, Igor V.; Göttlicher, Jörg; Polekhovsky, Yury S.; Rusakov, Vyacheslav S.; Nelyubina, Yuliya V.; Van, Konstantin V.

    2016-07-01

    A new mineral ferricoronadite with the simplified formula Pb(Mn6 4+Fe2 3+)O16 was discovered in the orogenetic zone related to the "Mixed Series" metamorphic complex near the Nežilovo village, Pelagonian massif, Republic of Macedonia. Associated minerals are franklinite, gahnite, hetaerolite, roméite, almeidaite, Mn-analogue of plumboferrite, zincohögbomite analogue with Fe3+ > Al, zincochromite, Zn-bearing talc, Zn-bearing muscovite, baryte, quartz and zircon. Ferricoronadite is a late hydrothermal mineral forming veinlets up to 8 mm thick in granular aggregate predominantly composed by zinc-dominant spinels. The new mineral is opaque, black, with brownish black streak. The luster is strong submetallic to metallic. The micro-indentation hardness is 819 kg/mm2. Distinct cleavage is observed on (100). Ferricoronadite is brittle, with uneven fracture. The density calculated from the empirical formula is 5.538 g/cm3. In reflected light, ferricoronadite is light gray. The reflectance values [ R max/ R min, % ( λ, nm)] are: 28.7/27.8 (470), 27.6/26.6 (546), 27.2/26.1 (589), 26.5/25.5 (650). The IR spectrum shows the absence of H2O and OH groups. According to the Mössbauer spectrum, all iron is trivalent. The Mn K-edge XANES spectroscopy shows that Mn is predominantly tetravalent, with subordinate Mn3+. The chemical composition is (wt%; electron microprobe, Mn apportioned between MnO2 and Mn2O3 based on the charge-balance requirement): BaO 5.16, PbO 24.50, ZnO 0.33, Al2O3 0.50, Mn2O3 9.90, Fe2O3 11.45, TiO2 4.19, MnO2 44.81, total 100.84. The empirical formula based on 8 cations Mn + Fe + Ti + Al + Zn pfu is Pb1.03Ba0.32(Mn 4.85 4+ Fe 1.35 3+ Mn 1.18 3+ Ti0.49Al0.09Zn0.04)Σ8.00O16. The crystal structure was determined using single-crystal X-ray diffraction data. The new mineral is tetragonal, space group I4/ m, a = 9.9043(7), c = 2.8986(9) Å, V = 284.34(9) Å3, Z = 1. In ferricoronadite, double chains of edge-sharing (Mn, Fe, Ti)-centered octahedra are connected

  17. The 2012 August 11 MW 6.5, 6.4 Ahar-Varzghan earthquakes, NW Iran: aftershock sequence analysis and evidence for activity migration

    NASA Astrophysics Data System (ADS)

    Rezapour, Mehdi

    2016-02-01

    The Ahar-Varzghan doublet earthquakes with magnitudes MW 6.5 and 6.4 occurred on 2012 August 11 in northwest Iran and were followed by many aftershocks. In this paper, we analyse ˜5 months of aftershocks of these events. The Ahar-Varzghan earthquakes occurred along complex faults and provide a new constraint on the earthquake hazard in northwest Iran. The general pattern of relocated aftershocks defines a complex seismic zone covering an area of approximately 25 × 10 km2. The Ahar-Varzghan aftershock sequence shows a secondary activity which started on November 7, approximately 3 months after the main shocks, with a significant increase in activity, regarding both number of events and their magnitude. This stage was characterized by a seismic zone that propagated to the west of the main shocks. The catalogue of aftershocks for the doublet earthquake has a magnitude completeness of Mc 2.0. A below-average b-value for the Ahar-Varzghan sequence indicates a structural heterogeneity in the fault plane and the compressive stress state of the region. Relocated aftershocks occupy a broad zone clustering east-west with near-vertical dip which we interpret as the fault plane of the first of the doublet main shocks (MW 6.5). The dominant depth range of the aftershocks is from 3 to about 20 km, and the focal depths decrease toward the western part of the fault. The aftershock activity has its highest concentration in the eastern and middle parts of the active fault, and tapers off toward the western part of the active fault segment, indicating mainly a unilateral rupture toward west.

  18. Thrust faulting and 3D ground deformation of the 3 July 2015 Mw 6.4 Pishan, China earthquake from Sentinel-1A radar interferometry

    NASA Astrophysics Data System (ADS)

    Sun, Jianbao; Shen, Zheng-Kang; Li, Tao; Chen, Jie

    2016-06-01

    Boosted by the launch of Sentinel-1A radar satellite from the European Space Agency (ESA), we now have the opportunity of fast, full and multiple coverage of the land based deformation field of earthquakes. Here we use the data to investigate a strong earthquake struck Pishan, western China on July 3, 2015. The earthquake fault is blind and no ground break features are found on-site, thus Synthetic Aperture Radar (SAR) data give full play to its technical advantage for the recovery of coseismic deformation field. By using the Sentinel-1A radar data in the Interferometric Wide Swath mode, we obtain 3 tracks of InSAR data over the struck region, and resolve the 3D ground deformation generated by the earthquake. Then the Line-of-Sight (LOS) InSAR data are inverted for the slip-distribution of the seismogenic fault. The final model shows that the earthquake is completely blind with pure-thrust motion. The maximum slip is ~ 0.48 m at a depth of ~ 7 km, consistent with the depth estimate from seismic reflection data. In particular, the inverted model is also compatible with a south-dipping fault ramp among a group of fault interfaces detected by the seismic reflection profile over the region. The seismic moment obtained equals to a Mw 6.4 earthquake. The Pishan earthquake ruptured the frontal part of the thrust ramps under the Slik anticline, and unloaded the coulomb stress of them. However, it may have loaded stress to the back-thrust above the thrust ramps by ~ 1-4 bar, and promoted it for future failure. Moreover, the stress loading on the west side of the earthquake fault is much larger than that on the east side, indicating a higher risk for failure to the west of the Zepu fault.

  19. Source model of the 2015 Mw 6.4 Pishan earthquake constrained by interferometric synthetic aperture radar and GPS: Insight into blind rupture in the western Kunlun Shan

    NASA Astrophysics Data System (ADS)

    He, Ping; Wang, Qi; Ding, Kaihua; Wang, Min; Qiao, Xuejun; Li, Jie; Wen, Yangmao; Xu, Caijun; Yang, Shaomin; Zou, Rong

    2016-02-01

    The Pishan, Xinjiang, earthquake on 3 July 2015 is the one of largest events (Mw 6-7) that has occurred along the western Kunlun Shan, northwestern edge of the Tibetan Plateau in recent time. It involved blind thrusting at a shallow depth beneath the range front, providing a rare chance to gain insights into the interaction between the Tarim Basin and the Tibetan Plateau. Here we present coseismic ground displacements acquired by high-resolution ALOS-2 SAR imagery and derived from GPS resurveys on several near-field geodetic markers after the event. We observed a maximum displacement exceeding 10 cm in the epicentral region. Analysis of the data based on a finite fault model indicates that coseismic slip occurred on a subsurface plane of 22 km × 8 km in size with a dip of about 27° to the north and a strike of 114°, representing partial break of one ramp fault buried in Paleozoic strata at 8-16 km depths beneath the foothill of the western Kunlun Shan. This blind rupture is characterized largely by a compact thrusting patch with a peak slip of 0.63 m, resulting in a stress drop of 2.3 MPa. The source model yields a geodetic moment of 5.05 × 1018 N · m, corresponding to Mw 6.4. The Pishan earthquake suggests a northward migration of deformation front of the Tibetan Plateau onto the Tarim Basin. Our finding highlights slip along ramp-décollement faults to build up the western Kunlun Shan as the Tarim slab is subducting beneath western Tibet.

  20. REDSHIFT 6.4 HOST GALAXIES OF 10{sup 8} SOLAR MASS BLACK HOLES: LOW STAR FORMATION RATE AND DYNAMICAL MASS

    SciTech Connect

    Willott, Chris J.; Omont, Alain; Bergeron, Jacqueline

    2013-06-10

    We present Atacama Large Millimeter Array observations of rest-frame far-infrared continuum and [C II] line emission in two z = 6.4 quasars with black hole masses of Almost-Equal-To 10{sup 8} M{sub Sun }. CFHQS J0210-0456 is detected in the continuum with a 1.2 mm flux of 120 {+-} 35 {mu}Jy, whereas CFHQS J2329-0301 is undetected at a similar noise level. J2329-0301 has a star formation rate limit of <40 M{sub Sun} yr{sup -1}, considerably below the typical value at all redshifts for this bolometric luminosity. Through comparison with hydro simulations, we speculate that this quasar is observed at a relatively rare phase where quasar feedback has effectively shut down star formation in the host galaxy. [C II] emission is also detected only in J0210-0456. The ratio of [C II] to far-infrared luminosity is similar to that of low-redshift galaxies of comparable luminosity, suggesting that the previous finding of an offset in the relationships between this ratio and far-infrared luminosity at low and high redshifts may be partially due to a selection effect due to the limited sensitivity of previous continuum data. The [C II] line of J0210-0456 is relatively narrow (FWHM = 189 {+-} 18 km s{sup -1}), indicating a dynamical mass substantially lower than expected from the local black hole-velocity dispersion correlation. The [C II] line is marginally resolved at 0.''7 resolution with the blue and red wings spatially offset by 0.''5 (3 kpc) and a smooth velocity gradient of 100 km s{sup -1} across a scale of 6 kpc, possibly due to the rotation of a galaxy-wide disk. These observations are consistent with the idea that stellar mass growth lags black hole accretion for quasars at this epoch with respect to more recent times.

  1. A comparison of classical and intelligent methods to detect potential thermal anomalies before the 11 August 2012 Varzeghan, Iran, earthquake (Mw = 6.4)

    NASA Astrophysics Data System (ADS)

    Akhoondzadeh, M.

    2013-04-01

    In this paper, a number of classical and intelligent methods, including interquartile, autoregressive integrated moving average (ARIMA), artificial neural network (ANN) and support vector machine (SVM), have been proposed to quantify potential thermal anomalies around the time of the 11 August 2012 Varzeghan, Iran, earthquake (Mw = 6.4). The duration of the data set, which is comprised of Aqua-MODIS land surface temperature (LST) night-time snapshot images, is 62 days. In order to quantify variations of LST data obtained from satellite images, the air temperature (AT) data derived from the meteorological station close to the earthquake epicenter has been taken into account. For the models examined here, results indicate the following: (i) ARIMA models, which are the most widely used in the time series community for short-term forecasting, are quickly and easily implemented, and can efficiently act through linear solutions. (ii) A multilayer perceptron (MLP) feed-forward neural network can be a suitable non-parametric method to detect the anomalous changes of a non-linear time series such as variations of LST. (iii) Since SVMs are often used due to their many advantages for classification and regression tasks, it can be shown that, if the difference between the predicted value using the SVM method and the observed value exceeds the pre-defined threshold value, then the observed value could be regarded as an anomaly. (iv) ANN and SVM methods could be powerful tools in modeling complex phenomena such as earthquake precursor time series where we may not know what the underlying data generating process is. There is good agreement in the results obtained from the different methods for quantifying potential anomalies in a given LST time series. This paper indicates that the detection of the potential thermal anomalies derive credibility from the overall efficiencies and potentialities of the four integrated methods.

  2. Stochastic finite-fault simulation of ground motion from the August 11, 2012, M w 6.4 Ahar earthquake, northwestern Iran

    NASA Astrophysics Data System (ADS)

    Heidari, Reza

    2016-04-01

    In this study, the 11 August 2012 M w 6.4 Ahar earthquake is investigated using the ground motion simulation based on the stochastic finite-fault model. The earthquake occurred in northwestern Iran and causing extensive damage in the city of Ahar and surrounding areas. A network consisting of 58 acceleration stations recorded the earthquake within 8-217 km of the epicenter. Strong ground motion records from six significant well-recorded stations close to the epicenter have been simulated. These stations are installed in areas which experienced significant structural damage and humanity loss during the earthquake. The simulation is carried out using the dynamic corner frequency model of rupture propagation by extended fault simulation program (EXSIM). For this purpose, the propagation features of shear-wave including {Q}_s value, kappa value {k}_0 , and soil amplification coefficients at each site are required. The kappa values are obtained from the slope of smoothed amplitude of Fourier spectra of acceleration at higher frequencies. The determined kappa values for vertical and horizontal components are 0.02 and 0.05 s, respectively. Furthermore, an anelastic attenuation parameter is derived from energy decay of a seismic wave by using continuous wavelet transform (CWT) for each station. The average frequency-dependent relation estimated for the region is Q=(122± 38){f}^{(1.40± 0.16)}. Moreover, the horizontal to vertical spectral ratio H/V is applied to estimate the site effects at stations. Spectral analysis of the data indicates that the best match between the observed and simulated spectra occurs for an average stress drop of 70 bars. Finally, the simulated and observed results are compared with pseudo acceleration spectra and peak ground motions. The comparison of time series spectra shows good agreement between the observed and the simulated waveforms at frequencies of engineering interest.

  3. Interplate seismicity at the CRISP drilling site: The 2002 Mw 6.4 Osa Earthquake at the southeastern end of the Middle America Trench

    NASA Astrophysics Data System (ADS)

    Arroyo, Ivonne G.; Grevemeyer, Ingo; Ranero, Cesar R.; von Huene, Roland

    2014-07-01

    investigate potential relations between variations in seafloor relief and age of the incoming plate and interplate seismicity. Westward from Osa Peninsula in Costa Rica, a major change in the character of the incoming Cocos Plate is displayed by abrupt lateral variations in seafloor depth and thermal structure. Here a Mw 6.4 thrust earthquake was followed by three aftershock clusters in June 2002. Initial relocations indicate that the main shock occurred fairly trenchward of most large earthquakes along the Middle America Trench off central Costa Rica. The earthquake sequence occurred while a temporary network of OBH and land stations ˜80 km to the northwest were deployed. By adding readings from permanent local stations, we obtain uncommon P wave coverage of a large subduction zone earthquake. We relocate this catalog using a nonlinear probabilistic approach within both, a 1-D and a 3-D P wave velocity models. The main shock occurred ˜25 km from the trench and probably along the plate interface at 5-10 km depth. We analyze teleseismic data to further constrain the rupture process of the main shock. The best depth estimates indicate that most of the seismic energy was radiated at shallow depth below the continental slope, supporting the nucleation of the Osa earthquake at ˜6 km depth. The location and depth coincide with the plate boundary imaged in prestack depth-migrated reflection lines shot near the nucleation area. Aftershocks propagated downdip to the area of a 1999 Mw 6.9 sequence and partially overlapped it. The results indicate that underthrusting of the young and buoyant Cocos Ridge has created conditions for interplate seismogenesis shallower and closer to the trench axis than elsewhere along the central Costa Rica margin.

  4. Relocation of the Mw 6.4 July 1, 2009 earthquake to the south of Crete and modeling of its associated small tsunami

    NASA Astrophysics Data System (ADS)

    Bocchini, Gian Maria; Papadopoulos, Gerassimos A.; Novikova, Tatiana; Karastathis, Vassilis K.; Mouzakiotis, Aggelos; Voulgaris, Nikolaos

    2016-04-01

    On July 1, 2009 (09:30 UTC) a Mw6.4 earthquake ruptured south of Crete Island triggering a small tsunami. Eyewitness reported the tsunami from Myrtos and Arvi Port, in the SE coast of Crete, and in Chrisi islet. In Arvi 4 or 5 wave arrivals were reported after a withdrawal of the sea of about 1 m. The sea disturbance lasted for about 1 h. The earthquake occurred as the result of the subduction of the oceanic African Plate beneath the continental Eurasian Plate along the Hellenic Subduction Zone (HSZ). South of Crete the Nubia-Aegean convergence rate (~3.5 cm/yr) is partially accommodated by low-angle (~20-25°) thrust faults at 20-40km depths and by steeper (>30°) reverse-faults at shallower depths. The area of interest has been struck by large magnitude earthquakes in historical times that in some cases triggered damaging tsunamis (e.g AD 1303). Routine earthquake locations performed by NOA do not provide good quality hypocenters for the area under investigation given the poor azimuthal coverage and the low density of the seismic stations. The 2009 earthquake, given its tsunamigenic nature, has been identified as a key event to study the central segment of the HSZ. We performed the relocation of the 2009 mainshock along with the seismicity of the area (ML>=3, period 2008-2015) using the NLLoc algorithm and testing several 1D velocity models available for the area and a 2D velocity model obtained from a published N-S seismic refraction profile across Crete. The hypocenters obtained from NLLoc have been subsequently relocated with HypoDD algorithm using catalog phase data. The results from the various relocation procedures showed a shallow hypocentral depth (12-17km) of the 2009 event and its likely intraplate nature. A set of hypocentral solutions were selected on the basis of minimum RMS and smaller errors with the aim to perform tsunami simulations with varying source parameters. Two different fault dips were used to discriminate between the intraplate (dip 32

  5. New constraints from seismology and geodesy on the Mw = 6.4 2008 Movri (Greece) earthquake: evidence for a growing strike-slip fault system

    NASA Astrophysics Data System (ADS)

    Serpetsidaki, A.; Elias, P.; Ilieva, M.; Bernard, P.; Briole, P.; Deschamps, A.; Lambotte, S.; Lyon-Caen, H.; Sokos, E.; Tselentis, G.-A.

    2014-09-01

    The 2008 Mw = 6.4 Movri earthquake ruptured a NNE right lateral strike-slip fault about 30 km south of the city of Patras. Although some strike-slip activity on minor faults was known, there was no tectonic evidence of large scale NS striking fault and such a large event was not anticipated. Following the event, a network of six stations was installed for 4 months in the epicentral area in order to monitor aftershocks and in particular the northern part of the rupture area closest to the city of Patras. We combine these new aftershock observations with GPS measurements of an already existing geodetic network in the area performed just after the earthquake, as well as with SAR interferograms, together with already published source studies, in order to refine already proposed models of this event. The combined data set allows defining much more accurately the lateral and vertical limits of the rupture. Its length inferred from geodesy is ˜15 km and its modelled upper edge ˜17 km. The seismic moment then constrains the lower edge to coincide, within a few kilometres, with the Moho interface. The absence of seismicity in the shallow crust above the co-seismic fault is interpreted as a result of the decoupling effect of possible presence of salt layers above the rupture area, near 14 to 16 km in depth, which favours our interpretation of an immature strike-slip fault system, compatible with the absence of surface ruptures. The immature character of this large crustal fault is further suggested by the high variability of focal mechanisms and of fault geometries deduced from aftershock clusters, in the strike direction. Its geometry and mechanism is consistent with the crustal shear, striking NNE, revealed by GPS in this region. This shear and faulting activity might be generated by the differential slip rate on the subduction interface, 50 km to the south, leading to a north-northeastward propagating strike-slip fault zone. The wide extension of the aftershock

  6. Pressurized liquid extraction-gas chromatography-mass spectrometry for confirming the photo-induced generation of dioxin-like derivatives and other cosmetic preservative photoproducts on artificial skin.

    PubMed

    Alvarez-Rivera, Gerardo; Llompart, Maria; Garcia-Jares, Carmen; Lores, Marta

    2016-04-01

    The stability and photochemical transformations of cosmetic preservatives in topical applications exposed to UV-light is a serious but poorly understood problem. In this study, a high throughput extraction and selective method based on pressurized liquid extraction (PLE) coupled to gas chromatography-mass spectrometry (GC-MS) was validated and applied to investigate the photochemical transformation of the antioxidant butylated hydroxytoluene (BHT), as well as the antimicrobials triclosan (TCS) and phenyl benzoate (PhBz) in an artificial skin model. Two sets of photodegradation experiments were performed: (i) UV-Irradiation (8W, 254nm) of artificial skin directly spiked with the target preservatives, and (ii) UV-irradiation of artificial skin after the application of a cosmetic cream fortified with the target compounds. After irradiation, PLE was used to isolate the target preservatives and their transformation products. The follow-up of the photodegradation kinetics of the parent preservatives, the identification of the arising by-products, and the monitorization of their kinetic profiles was performed by GC-MS. The photochemical transformation of triclosan into 2,8-dichloro-dibenzo-p-dioxin (2,8-DCDD) and other dioxin-like photoproducts has been confirmed in this work. Furthermore, seven BHT photoproducts, and three benzophenones as PhBz by-products, have been also identified. These findings reveal the first evidences of cosmetic ingredients phototransformation into unwanted photoproducts on an artificial skin model. PMID:26948762

  7. Pressurized liquid extraction-gas chromatography-mass spectrometry for confirming the photo-induced generation of dioxin-like derivatives and other cosmetic preservative photoproducts on artificial skin.

    PubMed

    Alvarez-Rivera, Gerardo; Llompart, Maria; Garcia-Jares, Carmen; Lores, Marta

    2016-04-01

    The stability and photochemical transformations of cosmetic preservatives in topical applications exposed to UV-light is a serious but poorly understood problem. In this study, a high throughput extraction and selective method based on pressurized liquid extraction (PLE) coupled to gas chromatography-mass spectrometry (GC-MS) was validated and applied to investigate the photochemical transformation of the antioxidant butylated hydroxytoluene (BHT), as well as the antimicrobials triclosan (TCS) and phenyl benzoate (PhBz) in an artificial skin model. Two sets of photodegradation experiments were performed: (i) UV-Irradiation (8W, 254nm) of artificial skin directly spiked with the target preservatives, and (ii) UV-irradiation of artificial skin after the application of a cosmetic cream fortified with the target compounds. After irradiation, PLE was used to isolate the target preservatives and their transformation products. The follow-up of the photodegradation kinetics of the parent preservatives, the identification of the arising by-products, and the monitorization of their kinetic profiles was performed by GC-MS. The photochemical transformation of triclosan into 2,8-dichloro-dibenzo-p-dioxin (2,8-DCDD) and other dioxin-like photoproducts has been confirmed in this work. Furthermore, seven BHT photoproducts, and three benzophenones as PhBz by-products, have been also identified. These findings reveal the first evidences of cosmetic ingredients phototransformation into unwanted photoproducts on an artificial skin model.

  8. Murine melanomas accelerated by a single UVR exposure carry photoproduct footprints but lack UV signature C>T mutations in critical genes.

    PubMed

    Mukhopadhyay, P; Ferguson, B; Muller, H K; Handoko, H Y; Walker, G J

    2016-06-23

    Ultraviolet radiation (UVR) exposure increases malignant melanoma (MM) risk, but in the context of acute, not cumulative exposure. C>T and CC>TT changes make up the overwhelming majority of single base substitutions (SBS) in MM DNA, as both precursor melanocytes and melanocytic lesions have incurred incidental exposures to sunlight. To study the mutagenic mechanisms by which acute sunburn accelerates MM, we sequenced the exomes of spontaneous and neonatal UVB-induced Cdk4-R24C::Tyr-NRASQ61K mouse MMs. UVR-induced MMs carried more SBSs than spontaneous MMs, but the levels of genomic instability, reflected by translocations and copy number changes, were not different. C>T/G>A was the most common SBS in spontaneous and UVR-induced MMs, only modestly increased in the latter. However, they tended to occur at the motif A/GpCpG (reflecting C>T transition due to spontaneous deamination of cytosine at CpG) in spontaneous MMs, and T/CpCpC/T (reflecting the effects of pyrimidine dimers on either side of the mutated C) in UVR-induced MMs. Unlike MMs associated with repetitive exposures, we observed no CC>TT changes. In addition, we also found UVR 'footprints' at T>A/A>Ts (at NpTpT) and T>C/A>G (at CpTpC). These footprints are also present in MMs from a chronic UVR mouse model, and in some human MMs, suggesting that they may be minor UVR signature changes. We found few significantly somatically mutated genes (~6 per spontaneous and 15 per UVR-induced melanoma) in addition to the Cdk4 and NRAS mutations already present. Trp53 was the most convincing recurrently mutated gene; however, in the UVR-induced MMs no Trp53 mutations were at C>T/G>A, suggesting that it was probably mutated during tumour progression, not directly induced by UVR photoproducts. The very low load of recurrent mutations convincingly induced by classical UVB-induced dimer photoproducts may support a role for cell extrinsic mechanisms, such as photoimmunosuppression and inflammation in driving MM after acute

  9. Murine melanomas accelerated by a single UVR exposure carry photoproduct footprints but lack UV signature C>T mutations in critical genes.

    PubMed

    Mukhopadhyay, P; Ferguson, B; Muller, H K; Handoko, H Y; Walker, G J

    2016-06-23

    Ultraviolet radiation (UVR) exposure increases malignant melanoma (MM) risk, but in the context of acute, not cumulative exposure. C>T and CC>TT changes make up the overwhelming majority of single base substitutions (SBS) in MM DNA, as both precursor melanocytes and melanocytic lesions have incurred incidental exposures to sunlight. To study the mutagenic mechanisms by which acute sunburn accelerates MM, we sequenced the exomes of spontaneous and neonatal UVB-induced Cdk4-R24C::Tyr-NRASQ61K mouse MMs. UVR-induced MMs carried more SBSs than spontaneous MMs, but the levels of genomic instability, reflected by translocations and copy number changes, were not different. C>T/G>A was the most common SBS in spontaneous and UVR-induced MMs, only modestly increased in the latter. However, they tended to occur at the motif A/GpCpG (reflecting C>T transition due to spontaneous deamination of cytosine at CpG) in spontaneous MMs, and T/CpCpC/T (reflecting the effects of pyrimidine dimers on either side of the mutated C) in UVR-induced MMs. Unlike MMs associated with repetitive exposures, we observed no CC>TT changes. In addition, we also found UVR 'footprints' at T>A/A>Ts (at NpTpT) and T>C/A>G (at CpTpC). These footprints are also present in MMs from a chronic UVR mouse model, and in some human MMs, suggesting that they may be minor UVR signature changes. We found few significantly somatically mutated genes (~6 per spontaneous and 15 per UVR-induced melanoma) in addition to the Cdk4 and NRAS mutations already present. Trp53 was the most convincing recurrently mutated gene; however, in the UVR-induced MMs no Trp53 mutations were at C>T/G>A, suggesting that it was probably mutated during tumour progression, not directly induced by UVR photoproducts. The very low load of recurrent mutations convincingly induced by classical UVB-induced dimer photoproducts may support a role for cell extrinsic mechanisms, such as photoimmunosuppression and inflammation in driving MM after acute

  10. Molecular and sensory mechanisms to mitigate sunlight-induced DNA damage in treefrog tadpoles.

    PubMed

    Schuch, André P; Lipinski, Victor M; Santos, Mauricio B; Santos, Caroline P; Jardim, Sinara S; Cechin, Sonia Z; Loreto, Elgion L S

    2015-10-01

    The increased incidence of solar ultraviolet B (UVB) radiation has been proposed as an environmental stressor, which may help to explain the enigmatic decline of amphibian populations worldwide. Despite growing knowledge regarding the UV-induced biological effects in several amphibian models, little is known about the efficacy of DNA repair pathways. In addition, little attention has been given to the interplay between these molecular mechanisms with other physiological strategies that avoid the damage induced by sunlight. Here, DNA lesions induced by environmental doses of solar UVB and UVA radiation were detected in genomic DNA samples of treefrog tadpoles (Hypsiboas pulchellus) and their DNA repair activity was evaluated. These data were complemented by monitoring the induction of apoptosis in blood cells and tadpole survival. Furthermore, the tadpoles' ability to perceive and escape from UV wavelengths was evaluated as an additional strategy of photoprotection. The results show that tadpoles are very sensitive to UVB light, which could be explained by the slow DNA repair rates for both cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6,4) pyrimidone photoproducts (6,4PPs). However, they were resistant to UVA, probably as a result of the activation of photolyases during UVA irradiation. Surprisingly, a sensory mechanism that triggers their escape from UVB and UVA light avoids the generation of DNA damage and helps to maintain the genomic integrity. This work demonstrates the genotoxic impact of both UVB and UVA radiation on tadpoles and emphasizes the importance of the interplay between molecular and sensory mechanisms to minimize the damage caused by sunlight.

  11. Crystal structures of ethyl 6-(4-methyl­phen­yl)-4-oxo-4H-chromene-2-carboxyl­ate and ethyl 6-(4-fluoro­phen­yl)-4-oxo-4H-chromene-2-carboxyl­ate

    PubMed Central

    Gomes, Ligia R.; Low, John Nicolson; Fernandes, Carlos; Gaspar, Alexandra; Borges, Fernanda

    2016-01-01

    The crystal structures of two chromone derivatives, viz. ethyl 6-(4-methyl­phen­yl)-4-oxo-4H-chromene-2-carboxyl­ate, C19H16O4, (1), and ethyl 6-(4-fluoro­phen­yl)-4-oxo-4H-chromene-2-carboxyl­ate C18H13FO4, (2), have been determined: (1) crystallizes with two mol­ecules in the asymmetric unit. A comparison of the dihedral angles beween the mean planes of the central chromone core with those of the substituents, an ethyl ester moiety at the 2-position and a para-substituted phenyl ring at the 6-position shows that each mol­ecule differs significantly from the others, even the two independent mol­ecules (a and b) of (1). In all three mol­ecules, the carbonyl groups of the chromone and the carboxyl­ate are trans-related. The supra­molecular structure of (1) involves only weak C—H⋯π inter­actions between H atoms of the substituent phenyl group and the phenyl group, which link mol­ecules into a chain of alternating mol­ecules a and b, and weak π–π stacking inter­actions between the chromone units. The packing in (2) involves C—H⋯O inter­actions, which form a network of two inter­secting ladders involving the carbonyl atom of the carboxyl­ate group as the acceptor for H atoms at the 7-position of the chromone ring and from an ortho-H atom of the exocyclic benzene ring. The carbonyl atom of the chromone acts as an acceptor from a meta-H atom of the exocyclic benzene ring. π–π inter­actions stack the mol­ecules by unit translation along the a axis. PMID:26870574

  12. UVB dose-toxicity thresholds and steady-state DNA-photoproduct levels during chronic irradiation of inbred Xenopus laevis tadpoles.

    PubMed

    Pandelova, Iovanna; Hewitt, Stephen R; Rollins-Smith, Louise A; Hays, John B

    2006-01-01

    Environmental stressors that severely impact some species more than others can alter ecosystems and threaten biodiversity. Genotoxic stress, such as solar UV-B irradiance, may induce levels of DNA damage at rates that exceed repair capacities in some species but remain below repair capacities in other species. Repair rates would seem to establish toxicity thresholds. We used inbred Xenopus laevis tadpoles in the laboratory to test the hypothesis that balances between rates of induction of cyclobutane pyrimidine dimers (CPDs; the major UV-B photoproduct in DNA) and rates of CPD removal (repair) can determine UV-B toxicity thresholds. As rates of chronic UV-B irradiance were progressively increased by decreased shielding of lamps, survival decreased sharply over a relatively narrow range of dose rates. Apparent toxicity thresholds were associated with large increases in steady-state CPD levels. Induction at twice the measured removal (repair) rate produced sustained high CPDs and 100% mortality. Induction at one-half the removal rate resulted in negligible CPD levels and low mortality. Increased intensity of visible radiation available to drive CPD photoreactivation, mimicking interspecies variation in DNA repair capacity, reduced steady-state CPD levels and increased survival at UV-B dose rates that were previously toxic, resulting in increased thresholds of apparent toxicity. We suggest that threshold effects due to DNA repair should generally be considered in assessments of effects of genotoxic agents on species-specific population decreases and human health risks. PMID:17205633

  13. Cross Section Measurements of Charged Pion Photoproduction in Hydrogen and Deuterium from 1.1 to 5.5 GeV

    SciTech Connect

    L.Y. Zhu; John Arrington; Todd Averett; Elizabeth Beise; John Calarco; Ting Chang; Jian-Ping Chen; Eugene Chudakov; Marius Coman; Benjamin Clasie; Christopher Crawford; Sonja Dieterich; Frank Dohrmann; Dipangkar Dutta; Kevin Fissum; Salvatore Frullani; Haiyan Gao; Ronald Gilman; Charles Glashausser; Javier Gomez; Kawtar Hafidi; Jens-ole Hansen; Douglas Higinbotham; Roy Holt; Cornelis De Jager; Xiaodong Jiang; Edward Kinney; Kevin Kramer; Gerfried Kumbartzki; John LeRose; Nilanga Liyanage; David Mack; Pete Markowitz; Kathy McCormick; David Meekins; Zein-Eddine Meziani; Robert Michaels; Joseph Mitchell; Sirish Nanda; David Potterveld; Ronald Ransome; Paul Reimer; Bodo Reitz; Arunava Saha; Elaine Schulte; Charles Seely; Simon Sirca; Steffen Strauch; Vincent Sulkosky; Branislav Vlahovic; Lawrence Weinstein; Krishni Wijesooriya; Claude Williamson; Bogdan Wojtsekhowski; Hong XIANG; Feng Xiong; Wang Xu; Jianning Zeng; Xiaochao Zheng

    2004-09-01

    The differential cross section for the {gamma}n {yields} {pi}{sup -}p and the {gamma}p {yields} {pi}{sup +}n processes were measured at Jefferson Lab. The photon energies ranged from 1.1 to 5.5 GeV, corresponding to center-of-mass energies from 1.7 to 3.4 GeV. The pion center-of-mass angles varied from 50 degree to 110 degree. The {pi}{sup -} and {pi}{sup +} photoproduction data both exhibit a global scaling behavior at high energies and high transverse momenta, consistent with the constituent counting rule prediction and the existing {pi}{sup +} data. The data suggest possible substructure of the scaling behavior, which might be oscillations around the scaling value. The data show an enhancement in the scaled cross section at center-of-mass energy near 2.2 GeV. The differential cross section ratios at high energies and high transverse momenta can be described by calculations based on one-hard-gluon-exchange diagrams.

  14. Photoproduction of Λ(1405) and Σ0(1385) on the proton at Eγ=1.5-2.4 GeV

    NASA Astrophysics Data System (ADS)

    Niiyama, M.; Fujimura, H.; Ahn, D. S.; Ahn, J. K.; Ajimura, S.; Bhang, H. C.; Chang, T. H.; Chang, W. C.; Chen, J. Y.; Daté, S.; Fukui, S.; Funahashi, H.; Hicks, K.; Horie, K.; Hotta, T.; Imai, K.; Ishikawa, T.; Kato, Y.; Kino, K.; Kohri, H.; Makino, S.; Matsumura, T.; Mibe, T.; Miwa, K.; Miyabe, M.; Muramatsu, N.; Nakamura, M.; Nakano, T.; Nakatsugawa, Y.; Ohashi, Y.; Oshuev, D. S.; Parker, J. D.; Saito, N.; Sawada, T.; Sugaya, Y.; Sumihama, M.; Tang, J. L.; Uchida, M.; Wang, C. W.; Yorita, T.; Yosoi, M.

    2008-09-01

    Differential cross sections for γp→K+Λ(1405) and γp→K+Σ0(1385) reactions have been measured in the photon energy range from 1.5 to 2.4 GeV and the angular range of 0.8photoproduction cross section. The line shapes of Λ(1405) measured in Σ+π- and Σ-π+ decay modes were different with each other, indicating a strong interference of the isospin 0 and 1 terms of the Σπ scattering amplitudes. The ratios of Λ(1405) production to Σ0(1385) production were measured in two photon energy ranges: near the production threshold (1.5

  15. Folic acid and its photoproducts, 6-formylpterin and pterin-6-carboxylic acid, as generators of reactive oxygen species in skin cells during UVA exposure.

    PubMed

    Juzeniene, Asta; Grigalavicius, Mantas; Ma, Li Wei; Juraleviciute, Marina

    2016-02-01

    Folic acid (FA) is the synthetic form of folate (vitamin B9), present in supplements and fortified foods. During ultraviolet (UV) radiation FA is degraded to 6-formylpterin (FPT) and pterin-6-carboxylic acid (PCA) which generate reactive oxygen species (ROS) and may be phototoxic. The aim of the present study was to investigate the production of ROS and phototoxicity of FA, FPT and PCA in skin cells during UVA exposure. The production of ROS and phototoxicity of FA, FPT and PCA were studied in the immortal human keratinocytes (HaCaT) and malignant skin cells (A431 and WM115) during UVA exposure. Increased ROS production and the photoinactivation of cells in vitro were observed during UVA exposure in the presence of FA, FPT and PCA. HPLC analysis revealed that 10 μM FA photodegradation was around 2.1 and 5.8-fold faster than that of 5 μM and 1 μM FA. Photodegradation of FA is concentration dependent, and even non-phototoxic doses of FA and its photoproducts, FPT and PCA, generate high levels of ROS in vitro. FA, FPT and PCA are phototoxic in vitro. The photodegradation of topical or unmetabolized FA during UV exposure via sunlight, sunbeds or phototherapy may lead to ROS production, to the cutaneous folate deficiency, skin photocarcinogenesis and other deleterious skin effects. Further studies are needed to confirm whether UV exposure can decrease cutaneous and serum folate levels in humans taking FA supplements or using cosmetic creams with FA.

  16. K*(892)0Λ and K+Σ*(1385)- Photoproduction on the Deuteron

    SciTech Connect

    Mattione, Paul

    2011-05-01

    Thirteen N* states have been well-established according to the Particle Data Group, but some relativized quark models predict that many more N* resonances exist. Diquark models predict that the N* spectrum is limited by a correlated quark-pair in the nucleon, but there is strong evidence for the existence of the N 3/2+(1900)** resonance, which is absent in diquark models. Measuring the spectrum of N* states will provide valuable information on the relevant degrees of freedom within the nucleons. Most of the experimental searches for the N* states have been conducted in the π N channel. Some models of baryon decays predict that most of the unobserved N* states couple somewhat weakly to the π N channel, and that some couple non-negligibly to the KY, K*Y, and KY* channels. Measurements of the cross sections and polarization observables of strangeness photoproduction reactions can provide additional information on the spectrum of N* states. These measurements can be used in coupled-channel partial-wave analyses that can provide simultaneous constraints on the N* resonance parameters from several channels. These analyses can also take into account hadronic rescattering, which is predicted to have a large effect on the measured cross sections. However, to determine the isospin decomposition of the photo-transition amplitudes to these channels, photoproduction measurements are necessary on both the proton and the neutron. Measurements of the differential cross sections of the γn\\rightarrow K*(892)0Λ and γn→ K+Σ*(1385)- reactions have been performed using data from the Jefferson Lab Hall B CLAS g13 experiment. No experimental cross section data have yet been published on the γ n\\rightarrow K*(892)0Λ reaction, and the only published cross section data on

  17. Photoproduction of {Lambda} and {Sigma}{sup 0} Hyperons off Protons in the Nucleon Resonance Region using CLAS at Jefferson Lab

    SciTech Connect

    John McNabb

    2002-12-01

    The differential cross section and hyperon recoil polarizations of the photoproduction of the ground state hyperons, {gamma} p {yields} K{sup +} {Lambda} and {gamma} p {yields} K{sup +} {Sigma}{sup 0} , have been measured with the CLAS at Jefferson Lab up to a photon energy in the lab of 2.325 GeV. The results for both channels show significantly larger cross section in the middle to forward angles than have been observed previously by the SAPHIR Collaboration. Both reactions show significantly more backward peaking in the angular distributions than has previously been possible to observe. The backward peaking hints that hyperon resonances in the u-channel play a significant role in the production mechanism. In addition, in the {gamma} p {yields} K{sup +} {Lambda} reaction, a previously unobserved bump in the cross section was observed at forward angles, centered on a W of 1.95 GeV with a width of approximately {Gamma} = 100 MeV. In both {gamma} p {yields} K{sup +} Y reactions the recoil polarization in the forward direction seems reasonably well reproduced by t-channel interferences in a Regge model calculation as well as hadrodynamic models that include kaon resonances in the t-channel. The recoil polarization for {gamma} p {yields} K{sup +} {Lambda} shows a significant enhancement around a W of 1.9 GeV in the backward angles, which is a sign of resonance activity in this vicinity. The polarization of {gamma} p {yields} K{sup +} {Sigma}{sup 0} at backward angles is, in contrast, less pronounced and mostly consistent with zero.

  18. Extracting the photoproduction cross sections off the neutron, via the γn→π-p reaction, from deuteron data with final-state interaction effects

    NASA Astrophysics Data System (ADS)

    Tarasov, V. E.; Briscoe, W. J.; Gao, H.; Kudryavtsev, A. E.; Strakovsky, I. I.

    2011-09-01

    The incoherent pion photoproduction reaction γd→π-pp is considered theoretically in a wide energy region Eth≤Eγ≤2700 MeV. The model applied contains the impulse approximation as well as the NN and πN final-state-interaction (FSI) amplitudes. The aim of the paper is to study a reliable way for getting the information on elementary γn→π-p reaction cross sections beyond the impulse approximation for γd→π-pp. For the elementary γN→πN, NN→NN, and πN→πN amplitudes, the results of The George Washington University (GW) Data Analysis Center (DAC) are used. There are no additional theoretical constraints. The calculated cross sections dσ/dΩ(γd→π-pp) are compared with existing data. The procedure used to extract information on the differential cross section dσ/dΩ(γn→π-p) on the neutron from the deuteron data using the FSI correction factor R is discussed. The calculations for R versus π-p center-of-mass (CM) angle θ1 of the outgoing pion are performed at different photon-beam energies with kinematic cuts for a “quasifree” process γn→π-p. The results show a sizable FSI effect R≠1 from the S-wave part of pp-FSI at small angles close to θ1˜0: this region narrows as the photon energy increases. At larger angles, the effect is small (|R-1|≪1) and agrees with estimations of FSI in the Glauber approach.

  19. Induction of long interspersed nucleotide element-1 (L1) retrotransposition by 6-formylindolo[3,2-b]carbazole (FICZ), a tryptophan photoproduct.

    PubMed

    Okudaira, Noriyuki; Iijima, Kenta; Koyama, Takayoshi; Minemoto, Yuzuru; Kano, Shigeyuki; Mimori, Akio; Ishizaka, Yukihito

    2010-10-26

    Long interspersed nucleotide element-1 (L1) is a retroelement comprising about 17% of the human genome, of which 80-100 copies are competent as mobile elements (retrotransposition: L1-RTP). Although the genetic structures modified during L1-RTP have been clarified, little is known about the cellular signaling cascades involved. Herein we found that 6-formylindolo[3,2-b]carbazole (FICZ), a tryptophan photoproduct postulated as a candidate physiological ligand of the aryl hydrocarbon receptor (AhR), induces L1-RTP. Notably, RNA-interference experiments combined with back-transfection of siRNA-resistant cDNAs revealed that the induction of L1-RTP by FICZ is dependent on AhR nuclear translocator-1 (ARNT1), a binding partner of AhR, and the activation of cAMP-responsive element-binding protein. However, our extensive analyses suggested that AhR is not required for L1-RTP. FICZ stimulated the interaction of the L1-encoded open reading frame-1 (ORF1) and ARNT1, and recruited ORF1 to chromatin in a manner dependent on the activation of mitogen-activated protein kinase. Along with our additional observations that the cellular cascades for FICZ-induced L1-RTP were different from those of L1-RTP triggered by DNA damage, we propose that the presence of the cellular machinery of ARNT1 mediates L1-RTP. A possible role of ARNT1-mediated L1-RTP in the adaptation of living organisms to environmental changes is discussed.

  20. Recombinagenic Processing of Uv-Light Photoproducts in Nonreplicating Phage DNA by the Escherichia Coli Methyl-Directed Mismatch Repair System

    PubMed Central

    Feng, W. Y.; Lee, E.; Hays, J. B.

    1991-01-01

    Nonreplicating λ phage DNA in homoimmune Escherichia coli lysogens provides a useful model system for study of processes that activate DNA for homologous recombination. We measured recombination by extracting phage DNA from infected cells, using it to transfect recA recipient cells, and scoring the frequency of recombinant infective centers. With unirradiated phage, recombinant frequencies were less than 0.1%. However, recombination could be increased over 300-fold by prior UV irradiation of the phages. The dependence of recombination on UvrA function varied greatly with UV dose. With phage irradiated to 20 J/m(2), recombinant frequencies in repressed infections of uvr(+) bacteria were one-fifth those in uvrA infections; with phages irradiated to 100 J/m(2), frequencies in uvr(+) infections were thirty times higher than in uvrA infections. Most UV-stimulated recombination in uvrA infections appeared to depend on the bacterial methyl-directed mismatch-repair system: frequencies were depressed 5-20-fold in uvrA bacteria also lacking MutH, MutL or MutS functions, and recombinant frequencies decreased with increasing GATC-adenine methylation of phage stocks. The biological activity of nonreplicating UV-irradiated phage DNA declined with time after infection of uvrA cells; this decline was photoproduct-dependent, more marked for undermethylated than overmethylated phage DNA, and depended on host MutHLS functions. In uvr(+) bacteria, where the UvrABC system provided an alternative, apparently less efficient, route to recombinagenic DNA, UV-stimulated recombinant frequencies were about twice as high in mutH or mutLS as in mut(+) cells, in agreement with hyper-rec mut effects previously described by others. PMID:1838344

  1. Hydrogen Photoproduction by Immobilized N2-Fixing Cyanobacteria: Understanding the Role of the Uptake Hydrogenase in the Long-Term Process

    PubMed Central

    Kosourov, Sergey; Leino, Hannu; Murukesan, Gayathri; Lynch, Fiona; Sivonen, Kaarina; Tsygankov, Anatoly A.; Aro, Eva-Mari

    2014-01-01

    We have investigated two approaches to enhance and extend H2 photoproduction yields in heterocystous, N2-fixing cyanobacteria entrapped in thin alginate films. In the first approach, periodic CO2 supplementation was provided to alginate-entrapped, N-deprived cells. N deprivation led to the inhibition of photosynthetic activity in vegetative cells and the attenuation of H2 production over time. Our results demonstrated that alginate-entrapped ΔhupL cells were considerably more sensitive to high light intensity, N deficiency, and imbalances in C/N ratios than wild-type cells. In the second approach, Anabaena strain PCC 7120, its ΔhupL mutant, and Calothrix strain 336/3 films were supplemented with N2 by periodic treatments of air, or air plus CO2. These treatments restored the photosynthetic activity of the cells and led to a high level of H2 production in Calothrix 336/3 and ΔhupL cells (except for the treatment air plus CO2) but not in the Anabaena PCC 7120 strain (for which H2 yields did not change after air treatments). The highest H2 yield was obtained by the air treatment of ΔhupL cells. Notably, the supplementation of CO2 under an air atmosphere led to prominent symptoms of N deficiency in the ΔhupL strain but not in the wild-type strain. We propose that uptake hydrogenase activity in heterocystous cyanobacteria not only supports nitrogenase activity by removing excess O2 from heterocysts but also indirectly protects the photosynthetic apparatus of vegetative cells from photoinhibition, especially under stressful conditions that cause an imbalance in the C/N ratio in cells. PMID:25015894

  2. Hydrogen photoproduction by immobilized n2-fixing cyanobacteria: understanding the role of the uptake hydrogenase in the long-term process.

    PubMed

    Kosourov, Sergey; Leino, Hannu; Murukesan, Gayathri; Lynch, Fiona; Sivonen, Kaarina; Tsygankov, Anatoly A; Aro, Eva-Mari; Allahverdiyeva, Yagut

    2014-09-01

    We have investigated two approaches to enhance and extend H2 photoproduction yields in heterocystous, N2-fixing cyanobacteria entrapped in thin alginate films. In the first approach, periodic CO2 supplementation was provided to alginate-entrapped, N-deprived cells. N deprivation led to the inhibition of photosynthetic activity in vegetative cells and the attenuation of H2 production over time. Our results demonstrated that alginate-entrapped ΔhupL cells were considerably more sensitive to high light intensity, N deficiency, and imbalances in C/N ratios than wild-type cells. In the second approach, Anabaena strain PCC 7120, its ΔhupL mutant, and Calothrix strain 336/3 films were supplemented with N2 by periodic treatments of air, or air plus CO2. These treatments restored the photosynthetic activity of the cells and led to a high level of H2 production in Calothrix 336/3 and ΔhupL cells (except for the treatment air plus CO2) but not in the Anabaena PCC 7120 strain (for which H2 yields did not change after air treatments). The highest H2 yield was obtained by the air treatment of ΔhupL cells. Notably, the supplementation of CO2 under an air atmosphere led to prominent symptoms of N deficiency in the ΔhupL strain but not in the wild-type strain. We propose that uptake hydrogenase activity in heterocystous cyanobacteria not only supports nitrogenase activity by removing excess O2 from heterocysts but also indirectly protects the photosynthetic apparatus of vegetative cells from photoinhibition, especially under stressful conditions that cause an imbalance in the C/N ratio in cells.

  3. Analysis of the 3d(sup 6)4s((sup 6)D)4f-5g supermultiplet of Fe I in laboratory and solar infrared spectra

    NASA Technical Reports Server (NTRS)

    Johansson, S.; Nave, G.; Geller, M.; Sauval, A. J.; Grevesse, N.; Schoenfeld, W. G.; Change, E. S.; Farmer, C. B.

    1994-01-01

    The combined laboratory and solar analysis of the highly excited subconfigurations 3d(sup 6)4s((sup 6)D)4f and 3d(sup 6)4s((sup 6)D)5g of Fe I has allowed us to classify 87 lines of the 4f-5g supermultiplet in the spectral region 2545-2585 per cm. The level structure of these JK-coupled configurations is predicted by semiempirical calculations and the quardrupolic approximation. Semiempirical gf-values have been calculated and are compared to gf-values derived from the solar spectrum. The solar analysis has shown that these lines, which should be much less sensitive than lower excitation lines to departures from Local Thermal Equilibrium (LTE) and to temperature uncertanties, lead to a solar abundance of iron which is consistent with the meteoritic value (A(sub Fe) = 7.51).

  4. Rh(I)-catalyzed intramolecular [2 + 2 + 1] cycloaddition of allenenes: Construction of bicyclo[4.3.0]nonenones with an angular methyl group and tricyclo[6.4.0.0]dodecenone.

    PubMed

    Inagaki, Fuyuhiko; Itoh, Naoya; Hayashi, Yujiro; Matsui, Yumi; Mukai, Chisato

    2011-04-07

    The [RhCl(CO)dppp](2)-catalyzed intramolecular carbonylative [2 + 2 + 1] cycloaddition of allenenes was developed to prepare bicyclo[4.3.0]nonenones possessing a methyl group at the ring junction, which is difficult to achieve by the Pauson-Khand reaction of the corresponding enynes. This method also provided a new procedure for the construction of the tricyclo[6.4.0.0(1,5)]dodecenone framework in a satisfactory yield.

  5. Preparation, patterning and luminescent properties of oxyapatite La9.33(SiO6)4O2:A(A = Eu3+, Tb3+, Ce3+) phosphor films by sol gel soft lithography

    NASA Astrophysics Data System (ADS)

    Han, X. M.; Lin, J.; Xing, R. B.; Fu, J.; Wang, S. B.; Han, Y. C.

    2003-04-01

    Silicate oxyapatite La9.33(SiO6)4O2:A (A = Eu3+, Tb3+ and/or Ce3+) phosphor films and their patterning were fabricated by a sol-gel process combined with soft lithography. X-ray diffraction(XRD), Fourier transform infrared spectroscopy, atomic force microscopy, optical microscopy and photoluminescence spectra, as well as lifetimes, were used to characterize the resulting films. The results of XRD indicated that the films began to crystallize at 800 °C and the crystallinity increased with the increase in annealing temperatures. Transparent nonpatterned phosphor films were uniform and crack-free, which mainly consisted of rodlike grains with a size between 150 and 210 nm. Patterned thin films with different bandwidths(20, 50µm) were obtained by the micromoulding in capillaries technique. The doped rare earth ions(Eu3+, Tb3+ and Ce3+) showed their characteristic emission in crystalline La9.33(SiO6)4O2 phosphor films, i.e. Eu3+5D 0 --7F J(J = 0, 1, 2, 3, 4), Tb3+5D 3,4 --7F J(J = 3, 4, 5, 6) and Ce3+ 5d(2D)-- 4f(2F 2/5,2F 2/7) emissions, respectively. Both the lifetimes and PL intensity of the Eu3+, Tb3+ ions increased with increasing annealing temperature from 800 to 1100 °C, and the optimum concentrations for Eu3+, Tb3+ were determined to be 9 and 7 mol% of La3+ in La9.33(SiO6)4O2 films, respectively. An energy transfer from Ce3+ to Tb3+ was observed in the La9.33(SiO6)4O2:Ce, Tb phosphor films, and the energy transfer efficiency was estimated as a function of Tb3+ concentration.

  6. Mutation of histidine 105 in the T1 domain of the potassium channel Kv2.1 disrupts heteromerization with Kv6.3 and Kv6.4.

    PubMed

    Mederos Y Schnitzler, Michael; Rinné, Susanne; Skrobek, Lennart; Renigunta, Vijay; Schlichthörl, Günter; Derst, Christian; Gudermann, Thomas; Daut, Jürgen; Preisig-Müller, Regina

    2009-02-13

    The voltage-activated K(+) channel subunit Kv2.1 can form heterotetramers with members of the Kv6 subfamily, generating channels with biophysical properties different from homomeric Kv2.1 channels. The N-terminal tetramerization domain (T1) has been shown previously to play a role in Kv channel assembly, but the mechanisms controlling specific heteromeric assembly are still unclear. In Kv6.x channels the histidine residue of the zinc ion-coordinating C3H1 motif of Kv2.1 is replaced by arginine or valine. Using a yeast two-hybrid assay, we found that substitution of the corresponding histidine 105 in Kv2.1 by valine (H105V) or arginine (H105R) disrupted the interaction of the T1 domain of Kv2.1 with the T1 domains of both Kv6.3 and Kv6.4, whereas interaction of the T1 domain of Kv2.1 with itself was unaffected by this mutation. Using fluorescence resonance energy transfer (FRET), interaction could be detected between the subunits Kv2.1/Kv2.1, Kv2.1/Kv6.3, and Kv2.1/Kv6.4. Reduced FRET signals were obtained after co-expression of Kv2.1(H105V) or Kv2.1(H105R) with Kv6.3 or Kv6.4. Wild-type Kv2.1 but not Kv2.1(H105V) could be co-immunoprecipitated with Kv6.4. Co-expression of dominant-negative mutants of Kv6.3 reduced the current produced Kv2.1, but not of Kv2.1(H105R) mutants. Co-expression of Kv6.3 or Kv6.4 with wt Kv2.1 but not with Kv2.1(H105V) or Kv2.1(H105R) changed the voltage dependence of activation of the channels. Our results suggest that His-105 in the T1 domain of Kv2.1 is required for functional heteromerization with members of the Kv6 subfamily. We conclude from our findings that Kv2.1 and Kv6.x subunits have complementary T1 domains that control selective heteromerization. PMID:19074135

  7. Photoproduction of J/psi and of High Mass e+e- in Ultra-Peripheral Au+Au Collisions at sqrt SNN = 200 GeV

    SciTech Connect

    Afanasiev, S.; Awes, Terry C; Cianciolo, Vince; Enokizono, Akitomo; Read Jr, Kenneth F; Silvermyr, David O; Sorensen, Soren P; Stankus, Paul W; Young, Glenn R; PHENIX, Collaboration

    2009-01-01

    We present the first measurement of photoproduction of J/{Psi} and of two-photon production of high-mass e{sup +}e{sup -} pairs in electromagnetic (or ultra-peripheral) nucleus-nucleus interactions, using Au + Au data at {radical}S{sub NN} = 200 GeV. The events are tagged with forward neutrons emitted following Coulomb excitation of one or both Au* nuclei. The event sample consists of 28 events with m{sub e{sup +}e{sup -}} > 2 GeV/c{sup 2} with zero like-sign background. The measured cross sections at midrapidity of d{sigma}/dy (J/{Psi} + Xn,y = 0) = 76 {+-} 33(stat) {+-} 11(syst) {micro}b and d{sup 2}{sigma}/dm dy(e{sup +}e{sup -} + Xn, y = 0) = 86 {+-} 23(stat) {+-} 16(syst) {micro}b/(GeV/c{sup 2}) for m{sub e{sup +}e{sup -}} {element_of} [2.0,2.8] GeV/c{sup 2} have been compared and found to be consistent with models for photoproduction of J/{Psi} and QED based calculations of two-photon production of e{sup +}e{sup -} pairs.

  8. Characterization of the ultraviolet-visible photoproducts of thiophanate-methyl using high performance liquid chromatography coupled with high resolution tandem mass spectrometry-Detection in grapes and tomatoes.

    PubMed

    Chayata, Houda; Lassalle, Yannick; Nicol, Édith; Manolikakes, Sophia; Souissi, Yasmine; Bourcier, Sophie; Gosmini, Corinne; Bouchonnet, Stéphane

    2016-04-01

    UV-visible irradiation of thiophanate-methyl (TM) led to the formation of nine photoproducts that were characterized by high performance liquid chromatography coupled with high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). Although carbendazime has been reported in the literature to be the major metabolite and photoproduct of thiophanate-methyl, it was not detected in this study. However, an isomer of carbendazime referred as PP2, which was unambiguously characterized owing to CID experiments, was found in great abundance. Grape berries and cherry tomatoes treated with aqueous solutions of thiophanate-methyl were submitted to irradiation under laboratory conditions. TM and PP2 were detected in both peel and flesh of berries. The ability of TM and PP2 to pass through the fruit skin has been shown to be highly compound and matrix dependent. In vitro bioassays on Vibrio fischeri bacteria showed that the global ecotoxicity of the TM solution increases significantly with the irradiation time. PP2 should likely contribute to this ecotoxicity enhancement since in silico estimations for Daphnia magna provide a LC50 value seven times lower for PP2 than for the parent molecule. PMID:26961913

  9. Sequence context-dependent replication of DNA templates containing UV-induced lesions by human DNA polymerase iota.

    PubMed

    Vaisman, Alexandra; Frank, Ekaterina G; Iwai, Shigenori; Ohashi, Eiji; Ohmori, Haruo; Hanaoka, Fumio; Woodgate, Roger

    2003-09-18

    Humans possess four Y-family polymerases: pols eta, iota, kappa and the Rev1 protein. The pivotal role that pol eta plays in protecting us from UV-induced skin cancers is unquestioned given that mutations in the POLH gene (encoding pol eta), lead to the sunlight-sensitive and cancer-prone xeroderma pigmentosum variant phenotype. The roles that pols iota, kappa and Rev1 play in the tolerance of UV-induced DNA damage is, however, much less clear. For example, in vitro studies in which the ability of pol iota to bypass UV-induced cyclobutane pyrimidine dimers (CPDs) or 6-4 pyrimidine-pyrimidone (6-4PP) lesions has been assayed, are somewhat varied with results ranging from limited misinsertion opposite CPDs to complete lesion bypass. We have tested the hypothesis that such discrepancies might have arisen from different assay conditions and local sequence contexts surrounding each UV-photoproduct and find that pol iota can facilitate significant levels of unassisted highly error-prone bypass of a T-T CPD, particularly when the lesion is located in a 3'-A[T-T]A-5' template sequence context and the reaction buffer contains no KCl. When encountering a T-T 6-4PP dimer under the same assay conditions, pol iota efficiently and accurately inserts the correct base, A, opposite the 3'T of the 6-4PP by factors of approximately 10(2) over the incorporation of incorrect nucleotides, while incorporation opposite the 5'T is highly mutagenic. Pol kappa has been proposed to function in the bypass of UV-induced lesions by helping extend primers terminated opposite CPDs. However, we find no evidence that the combined actions of pol iota and pol kappa result in a significant increase in bypass of T-T CPDs when compared to pol iota alone. Our data suggest that under certain conditions and sequence contexts, pol iota can bypass T-T CPDs unassisted and can efficiently incorporate one or more bases opposite a T-T 6-4PP. Such biochemical activities may, therefore, be of biological

  10. 24 CFR 6.4 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... at 24 CFR part 8 apply to discrimination on the basis of disability; and the regulations at 24 CFR... Recipient shall not be prohibited by this section from taking any action eligible under subpart C of 24 CFR... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Discrimination prohibited....

  11. 24 CFR 6.4 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... at 24 CFR part 8 apply to discrimination on the basis of disability; and the regulations at 24 CFR... Recipient shall not be prohibited by this section from taking any action eligible under subpart C of 24 CFR... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Discrimination prohibited....

  12. 24 CFR 6.4 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... at 24 CFR part 8 apply to discrimination on the basis of disability; and the regulations at 24 CFR... Recipient shall not be prohibited by this section from taking any action eligible under subpart C of 24 CFR... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Discrimination prohibited....

  13. 24 CFR 6.4 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... at 24 CFR part 8 apply to discrimination on the basis of disability; and the regulations at 24 CFR... Recipient shall not be prohibited by this section from taking any action eligible under subpart C of 24 CFR... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Discrimination prohibited....

  14. 24 CFR 6.4 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... at 24 CFR part 8 apply to discrimination on the basis of disability; and the regulations at 24 CFR... Recipient shall not be prohibited by this section from taking any action eligible under subpart C of 24 CFR... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Discrimination prohibited....

  15. PYTHIA 6.4 Physics and Manual

    SciTech Connect

    Sjostrand, Torbjorn; Mrenna, Stephen; Skands, Peter; /Fermilab

    2006-03-01

    The Pythia program can be used to generate high-energy-physics ''events'', i.e. sets of outgoing particles produced in the interactions between two incoming particles. The objective is to provide as accurate as possible a representation of event properties in a wide range of reactions, within and beyond the Standard Model, with emphasis on those where strong interactions play a role, directly or indirectly, and therefore multihadronic final states are produced. The physics is then not understood well enough to give an exact description; instead the program has to be based on a combination of analytical results and various QCD-based models. This physics input is summarized here, for areas such as hard subprocesses, initial- and final-state parton showers, underlying events and beam remnants, fragmentation and decays, and much more. Furthermore, extensive information is provided on all program elements: subroutines and functions, switches and parameters, and particle and process data. This should allow the user to tailor the generation task to the topics of interest.

  16. Nucleotide Excision Repair and Vitamin D—Relevance for Skin Cancer Therapy

    PubMed Central

    Pawlowska, Elzbieta; Wysokinski, Daniel; Blasiak, Janusz

    2016-01-01

    Ultraviolet (UV) radiation is involved in almost all skin cancer cases, but on the other hand, it stimulates the production of pre-vitamin D3, whose active metabolite, 1,25-dihydroxyvitamin D3 (1,25VD3), plays important physiological functions on binding with its receptor (vitamin D receptor, VDR). UV-induced DNA damages in the form of cyclobutane pyrimidine dimers or (6-4)-pyrimidine-pyrimidone photoproducts are frequently found in skin cancer and its precursors. Therefore, removing these lesions is essential for the prevention of skin cancer. As UV-induced DNA damages are repaired by nucleotide excision repair (NER), the interaction of 1,25VD3 with NER components can be important for skin cancer transformation. Several studies show that 1,25VD3 protects DNA against damage induced by UV, but the exact mechanism of this protection is not completely clear. 1,25VD3 was also shown to affect cell cycle regulation and apoptosis in several signaling pathways, so it can be considered as a potential modulator of the cellular DNA damage response, which is crucial for mutagenesis and cancer transformation. 1,25VD3 was shown to affect DNA repair and potentially NER through decreasing nitrosylation of DNA repair enzymes by NO overproduction by UV, but other mechanisms of the interaction between 1,25VD3 and NER machinery also are suggested. Therefore, the array of NER gene functioning could be analyzed and an appropriate amount of 1.25VD3 could be recommended to decrease UV-induced DNA damage important for skin cancer transformation. PMID:27058533

  17. A model for triplet mutation formation based on error-prone translesional DNA synthesis opposite UV photolesions.

    PubMed

    Ikehata, Hironobu; Ono, Tetsuya; Tanaka, Kiyoji; Todo, Takeshi

    2007-05-01

    A triplet mutation is defined as multiple base substitutions or frameshifts within a three-nucleotide sequence which includes a dipyrimidine sequence. Triplet mutations have recently been identified as a new type of UV-specific mutation, although the mechanism of their formation is unknown. A total of 163 triplet mutations were identified through an extensive search of previously published data on UV-induced mutations, including mutations from skin, skin cancer, and cultured mammalian cells. Seven common patterns of sequence changes were found: Type I, NTC-->TTT; Type IIa, NCC-->PyTT or PyCT (Py, pyrimidine); Type IIb, TCC-->PuTT or PuCT (Pu, purine); Type III, NCC-->NAT or NTA; Type IV, NTT-->AAT; Type Va, NCT-->NTX; and Type Vb, PuCT-->XTT (N and X, independent anonymous bases). Furthermore, it is suggested that the type of UV lesion responsible for each of these triplet mutation classes are (a) pyrimidine(6-4)pyrimidone photoproducts for Types I, IIb, III, IV and Vb, (b) cyclobutane pyrimidine dimers for Type Va, and (c) Dewar valence isomers for Types IIa and IIb. These estimations are based primarily on results from previous studies using photolyases specific for each type of UV lesion. A model is proposed to explain the formation of each type of triplet mutation, based on error-prone translesional DNA synthesis opposite UV-specific photolesions. The model is largely consistent with the 'A-rule', and predicts error-prone insertions not only opposite photolesions but also opposite the undamaged template base one-nucleotide downstream from the lesions.

  18. Discovery of 6-({4-[2-(4-tert-butylphenyl)-1H-benzimidazol-4-yl]piperazin-1-yl}methyl)quinoxaline (WAY-207024): an orally active antagonist of the gonadotropin releasing hormone receptor (GnRH-R).

    PubMed

    Pelletier, Jeffrey C; Chengalvala, Murty V; Cottom, Joshua E; Feingold, Irene B; Green, Daniel M; Hauze, Diane B; Huselton, Christine A; Jetter, James W; Kopf, Gregory S; Lundquist, Joseph T; Magolda, Ronald L; Mann, Charles W; Mehlmann, John F; Rogers, John F; Shanno, Linda K; Adams, William R; Tio, Cesario O; Wrobel, Jay E

    2009-04-01

    A potent, highly insoluble, GnRH antagonist with a 2-phenyl-4-piperazinylbenzimidazole template and a quinoxaline-2,3-dione pharmacophore was modified to maintain GnRH antagonist activity and improve in vitro pharmaceutical properties. Structural changes to the quinoxaline-2,3-dione portion of the molecule resulted in several structures with improved properties and culminated in the discovery of 6-([4-[2-(4-tert-butylphenyl)-1H-benzimidazol-4-yl]piperazin-1-yl] methyl)quinoxaline (WAY-207024). The compound was shown to have excellent pharmacokinetic parameters and lowered rat plasma LH levels after oral administration.

  19. High antiallergic activity of 5,6,4'-trihydroxy-7,8,3'-trimethoxyflavone and 5,6-dihydroxy-7,8,3',4'-tetramethoxyflavone from eau de cologne mint (Mentha×piperita citrata).

    PubMed

    Sato, Akihiko; Tamura, Hirotoshi

    2015-04-01

    The following compounds with higher antiallergic activities were isolated from eau de cologne mint leaves: 5,6,4'-trihydroxy-7,8-dimethoxyflavone (6), 5,6,4'-trihydroxy-7,8,3'-trimethoxyflavone (7), 5,6-dihydroxy-7,3',4'-trimethoxyflavone (8), 5,6-dihydroxy-7,8,3',4'-tetramethoxyflavone (9), and 5,6-dihydroxy-7,8,4'-trimethoxyflavone (10). The IC50 values of compounds 6-10 against RBL-2H3 cells were 6.7, 2.4, 5.6, 3.0, and 6.1μM. Compounds 7 and 9 (IC50 2.4μM and 3.0μM) had the highest antiallergic activities among the flavonoids previously reported. The amounts of 7, 9, and 10 isolated were fairly high, at 177.7mg/kg, 278.0mg/kg, and 179.7mg/kg in the mint, respectively. LD5 value (index of toxicity) and LD5/IC50 ratio of 7 and 9 indicate that the safety is greater than that of luteolin, a typical antiallergic substance. The extract containing powerful antiallergic flavones, 6-10 with higher hydrophobicity could be selectively separated from the extract containing luteolin and other flavonoid glycosides by partition with dichloromethane and water. Therefore, compounds 7 and 9 in mint, and the dichloromethane extract would be the most potent and preventive resources against type I allergy.

  20. A new insight into high-strength Ti62Nb12.2Fe13.6Co6.4Al5.8 alloys with bimodal microstructure fabricated by semi-solid sintering.

    PubMed

    Liu, L H; Yang, C; Kang, L M; Qu, S G; Li, X Q; Zhang, W W; Chen, W P; Li, Y Y; Li, P J; Zhang, L C

    2016-03-31

    It is well known that semi-solid forming could only obtain coarse-grained microstructure in a few alloy systems with a low melting point, such as aluminum and magnesium alloys. This work presents that semi-solid forming could also produce novel bimodal microstructure composed of nanostructured matrix and micro-sized (CoFe)Ti2 twins in a titanium alloy, Ti62Nb12.2Fe13.6Co6.4Al5.8. The semi-solid sintering induced by eutectic transformation to form a bimodal microstructure in Ti62Nb12.2Fe13.6Co6.4Al5.8 alloy is a fundamentally different approach from other known methods. The fabricated alloy exhibits high yield strength of 1790 MPa and plastic strain of 15.5%. The novel idea provides a new insight into obtaining nano-grain or bimodal microstructure in alloy systems with high melting point by semi-solid forming and into fabricating high-performance metallic alloys in structural applications.