Science.gov

Sample records for 8a 8b 8c

  1. 2p-2p decay of {sup 8}C and isospin-allowed 2p decay of the isobaric-analog state in {sup 8}B

    SciTech Connect

    Charity, R. J.; Elson, J. M.; Manfredi, J.; Shane, R.; Sobotka, L. G.; Chajecki, Z.; Coupland, D.; Iwasaki, H.; Kilburn, M.; Lee, Jenny; Lynch, W. G.; Sanetullaev, A.; Tsang, M. B.; Winkelbauer, J.; Youngs, M.; Marley, S. T.; Shetty, D. V.; Wuosmaa, A. H.; Ghosh, T. K.

    2010-10-15

    {sup 8}C is found to decay to four protons and an {alpha} particle in two 2p emission steps. The correlations between the protons in the first step ({sup 8}C to {sup 6}Be) exhibit a significant enhancement in the region of the decay phase space where the two protons have small relative energy, a region sometimes called the diproton region. The decay of the isobaric analog of {sup 8}C in {sup 8}B is also found to decay by 2p emission. This is the first case of isospin-allowed 2p decay between isobaric analog states.

  2. The New Structural Materials Science Beamlines BL8A and 8B at Photon Factory

    SciTech Connect

    Nakao, A.; Sugiyama, H.; Koyama, A.; Watanabe, K.

    2010-06-23

    BL8A and 8B are new beamlines for structural materials science at Photon Factory. The primary characteristics of both beamlines are similar. The incident beam is monochromatized by the Si(111) double-flat crystal monochromator and focused at the sample position by a Rh-coated bent cylindrical quartz mirror. The Weissenberg-camera-type imaging-plate (IP) diffractometers were installed. The X-ray diffraction experiments for structural studies of strongly correlated materials, such as transition metals, molecular conductors, endohedral fullerenes, nano-materials, etc, are conducted at these stations.

  3. MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.

    PubMed

    Wang, Youning; Li, Kexue; Chen, Liang; Zou, Yanmin; Liu, Haipei; Tian, Yinping; Li, Dongxiao; Wang, Rui; Zhao, Fang; Ferguson, Brett J; Gresshoff, Peter M; Li, Xia

    2015-07-01

    Legume root nodules convert atmospheric nitrogen gas into ammonium through symbiosis with a prokaryotic microsymbiont broadly called rhizobia. Auxin signaling is required for determinant nodule development; however, the molecular mechanism of auxin-mediated nodule formation remains largely unknown. Here, we show in soybean (Glycine max) that the microRNA miR167 acts as a positive regulator of lateral root organs, namely nodules and lateral roots. miR167c expression was up-regulated in the vasculature, pericycle, and cortex of soybean roots following inoculation with Bradyrhizobium japonicum strain USDA110 (the microsymbiont). It was found to positively regulate nodule numbers directly by repressing the target genes GmARF8a and GmARF8b (homologous genes of Arabidopsis [Arabidopsis thaliana] AtARF8 that encode auxin response factors). Moreover, the expression of miR167 and its targets was up- and down-regulated by auxin, respectively. The miR167-GmARF8 module also positively regulated nodulation efficiency under low microsymbiont density, a condition often associated with environmental stress. The regulatory role of miR167 on nodule initiation was dependent on the Nod factor receptor GmNFR1α, and it acts upstream of the nodulation-associated genes nodule inception, nodulation signaling pathway1, early nodulin40-1, NF-YA1 (previously known as HAEM activator protein2-1), and NF-YA2. miR167 also promoted lateral root numbers. Collectively, our findings establish a key role for the miR167-GmARF8 module in auxin-mediated nodule and lateral root formation in soybean. PMID:25941314

  4. MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development1[OPEN

    PubMed Central

    Wang, Youning; Li, Kexue; Chen, Liang; Zou, Yanmin; Tian, Yinping; Li, Dongxiao; Wang, Rui; Zhao, Fang; Ferguson, Brett J.; Gresshoff, Peter M.

    2015-01-01

    Legume root nodules convert atmospheric nitrogen gas into ammonium through symbiosis with a prokaryotic microsymbiont broadly called rhizobia. Auxin signaling is required for determinant nodule development; however, the molecular mechanism of auxin-mediated nodule formation remains largely unknown. Here, we show in soybean (Glycine max) that the microRNA miR167 acts as a positive regulator of lateral root organs, namely nodules and lateral roots. miR167c expression was up-regulated in the vasculature, pericycle, and cortex of soybean roots following inoculation with Bradyrhizobium japonicum strain USDA110 (the microsymbiont). It was found to positively regulate nodule numbers directly by repressing the target genes GmARF8a and GmARF8b (homologous genes of Arabidopsis [Arabidopsis thaliana] AtARF8 that encode auxin response factors). Moreover, the expression of miR167 and its targets was up- and down-regulated by auxin, respectively. The miR167-GmARF8 module also positively regulated nodulation efficiency under low microsymbiont density, a condition often associated with environmental stress. The regulatory role of miR167 on nodule initiation was dependent on the Nod factor receptor GmNFR1α, and it acts upstream of the nodulation-associated genes NODULE INCEPTION, NODULATION SIGNALING PATHWAY1, EARLY NODULIN40-1, NF-YA1 (previously known as HAEM ACTIVATOR PROTEIN2-1), and NF-YA2. miR167 also promoted lateral root numbers. Collectively, our findings establish a key role for the miR167-GmARF8 module in auxin-mediated nodule and lateral root formation in soybean. PMID:25941314

  5. MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.

    PubMed

    Wang, Youning; Li, Kexue; Chen, Liang; Zou, Yanmin; Liu, Haipei; Tian, Yinping; Li, Dongxiao; Wang, Rui; Zhao, Fang; Ferguson, Brett J; Gresshoff, Peter M; Li, Xia

    2015-07-01

    Legume root nodules convert atmospheric nitrogen gas into ammonium through symbiosis with a prokaryotic microsymbiont broadly called rhizobia. Auxin signaling is required for determinant nodule development; however, the molecular mechanism of auxin-mediated nodule formation remains largely unknown. Here, we show in soybean (Glycine max) that the microRNA miR167 acts as a positive regulator of lateral root organs, namely nodules and lateral roots. miR167c expression was up-regulated in the vasculature, pericycle, and cortex of soybean roots following inoculation with Bradyrhizobium japonicum strain USDA110 (the microsymbiont). It was found to positively regulate nodule numbers directly by repressing the target genes GmARF8a and GmARF8b (homologous genes of Arabidopsis [Arabidopsis thaliana] AtARF8 that encode auxin response factors). Moreover, the expression of miR167 and its targets was up- and down-regulated by auxin, respectively. The miR167-GmARF8 module also positively regulated nodulation efficiency under low microsymbiont density, a condition often associated with environmental stress. The regulatory role of miR167 on nodule initiation was dependent on the Nod factor receptor GmNFR1α, and it acts upstream of the nodulation-associated genes nodule inception, nodulation signaling pathway1, early nodulin40-1, NF-YA1 (previously known as HAEM activator protein2-1), and NF-YA2. miR167 also promoted lateral root numbers. Collectively, our findings establish a key role for the miR167-GmARF8 module in auxin-mediated nodule and lateral root formation in soybean.

  6. 15 CFR 8b.18 - New construction.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false New construction. 8b.18 Section 8b.18... HANDICAPPED IN FEDERALLY ASSISTED PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE Accessibility § 8b.18...

  7. 15 CFR 8b.18 - New construction.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false New construction. 8b.18 Section 8b.18... HANDICAPPED IN FEDERALLY ASSISTED PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE Accessibility § 8b.18...

  8. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  9. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  10. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  11. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  12. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  13. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  14. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  15. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  16. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  17. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  18. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  19. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  20. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  1. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  2. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  3. 15 CFR 8b.22 - Academic adjustments.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Academic adjustments. 8b.22 Section 8b... Secondary Education § 8b.22 Academic adjustments. (a) Academic requirements. A recipient to which this subpart applies shall make such modifications to its academic requirements as are necessary to ensure...

  4. 15 CFR 8b.22 - Academic adjustments.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Academic adjustments. 8b.22 Section 8b... Secondary Education § 8b.22 Academic adjustments. (a) Academic requirements. A recipient to which this subpart applies shall make such modifications to its academic requirements as are necessary to ensure...

  5. 15 CFR 8b.5 - Assurances required.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Assurances required. 8b.5 Section 8b.5... Assurances required. (a) Assurances. An applicant for Federal financial assistance to which this part applies shall submit an assurance, on a form specified by the Secretary, that the program or activity will...

  6. 15 CFR 8b.5 - Assurances required.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Assurances required. 8b.5 Section 8b.5... Assurances required. (a) Assurances. An applicant for Federal financial assistance to which this part applies shall submit an assurance, on a form specified by the Secretary, that the program or activity will...

  7. 15 CFR 8b.18 - New construction.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false New construction. 8b.18 Section 8b.18... construction. (a) Design and construction. Each facility or part of a facility constructed by, on behalf of,...

  8. 15 CFR 8c.40 - Employment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Equal Employment Opportunity Commission in 29 CFR Part 1613, shall apply to employment in federally... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Employment. 8c.40 Section 8c.40... BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF COMMERCE § 8c.40...

  9. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  10. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  11. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  12. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  13. Foothills Parkway Section 8B Final Environmental Report, Volume 2, Appendices A-C

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NW, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this EN there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constricting Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER, which consists of Appendices A, B, and C, assesses the potential geologic impacts of the proposed Section 8B construction, presents the results of the Section 8B soil survey, and describes the water quality studies and analyses performed for the ER. The following summary sections provide information for geology, soils, and water quality.

  14. 15 CFR 8c.60 - Communications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Communications. 8c.60 Section 8c.60 Commerce and Foreign Trade Office of the Secretary of Commerce ENFORCEMENT OF NONDISCRIMINATION ON THE... Communications. (a) The agency shall take appropriate steps to ensure effective communication with...

  15. 15 CFR 8c.60 - Communications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Communications. 8c.60 Section 8c.60 Commerce and Foreign Trade Office of the Secretary of Commerce ENFORCEMENT OF NONDISCRIMINATION ON THE... Communications. (a) The agency shall take appropriate steps to ensure effective communication with...

  16. 15 CFR 8c.60 - Communications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Communications. 8c.60 Section 8c.60 Commerce and Foreign Trade Office of the Secretary of Commerce ENFORCEMENT OF NONDISCRIMINATION ON THE... Communications. (a) The agency shall take appropriate steps to ensure effective communication with...

  17. 15 CFR 8c.60 - Communications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Communications. 8c.60 Section 8c.60 Commerce and Foreign Trade Office of the Secretary of Commerce ENFORCEMENT OF NONDISCRIMINATION ON THE... Communications. (a) The agency shall take appropriate steps to ensure effective communication with...

  18. Full genomic analyses of human rotavirus strains possessing the rare P[8]b VP4 subtype.

    PubMed

    Ghosh, Souvik; Paul, Shyamal Kumar; Yamamoto, Dai; Nagashima, Shigeo; Kobayashi, Nobumichi

    2011-08-01

    Rotaviruses with the P[8] VP4 genotype are a major cause of acute infantile diarrhea. The P[8] genotype is classified into two genetically distinct subtypes, P[8]a and P[8]b. Most of the P[8] strains belong to subtype P[8]a, whilst P[8]b strains are rare. To date, the whole genomes of a few P[8]a strains have been analyzed, whilst there are no reports on full genomic analysis of the P[8]b strains. To determine the genetic makeup of the rare P[8]b strains and their overall genetic relatedness to the P[8]a strains, the present study analyzed the full genomes of a human G9P[8]b strain, MMC38, and a G1P[8]b strain, MMC71, detected in Bangladesh in 2005. By nucleotide sequence identities and phylogenetic analyses, strains MMC38 and MMC71 exhibited a human rotavirus Wa-like genotype constellation. Except for the VP4 gene, all the genes of strains MMC38 and MMC71 were closely related to cognate genes of the contemporary and more recent human Wa-like G1P[8]a, G9P[8]a, G11P[8]a, G11P[25], G12P[6] and G12P[8]a strains, including those from Bangladesh. Therefore, strains MMC38 and MMC71 possessed the genetically distinct P[8]b VP4 gene on a common human Wa-like genetic backbone, pointing towards their possible origin from reassortment events between common human Wa-like strains and unidentified rotavirus strains possessing the rare P[8]b-like VP4 gene. Since strains with this stable Wa-like genetic backbone can spread rapidly, and it is not certain as to whether the current rotavirus vaccines will be equally efficacious against the P[8]b strains as the P[8]a strains, proper detection of P[8]b strains and their whole genomic analyses might be of public health significance. To our knowledge, the present study is the first report on full genomic analysis of the rare P[8]b rotavirus strains. PMID:21640848

  19. Foothills Parkway Section 8B Final Environmental Report, Volume 6, Appendix N

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER documents the results of the architectural, historical, and cultural resources assessment for the entire Section 8B ROW that was completed in May 1995 to document the architectural, historical, and cultural resources located within the project area. The assessment included evaluation of the potential for cultural (i.e., rural historic) landscapes in the area of the ROW. The assessment showed that one National Register-listed property is located 0.3 mile south of the ROW

  20. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Treatment of students. 8b.21 Section 8b.21 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall,...

  1. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Treatment of students. 8b.21 Section 8b.21 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall,...

  2. Foothills Parkway Section 8B Final Environmental Report, Volume 3, Appendix D

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER inventories the fishes and benthic macroinvertebrates inhabiting the aquatic ecosystems potentially affected by the proposed construction of Section 8B. Stream biological surveys were completed at 31 stream sites during the Fall of 1994. The sampling strategy for both invertebrates and fish was to survey the different taxa from all available habitats. For benthic invertebrates, a standardized qualitative manual collection technique was employed for all 31 stations. For fish

  3. Foothills Parkway Section 8B Final Environmental Report, Volume 5, Appendices J-M

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER consists of Appendices J through M, which describe potential impacts regarding traffic, noise, and aesthetics. The results of the traffic studies described in these appendices resulted in the following conclusions: Unacceptable levels of service will occur on numerous roads within and outside of the park increasingly in the Mure based upon current and future regional growth. However, the results of the traffic assessment indicated that there would be no significant or

  4. PROCEEDINGS: 1993 SO2 CONTROL SYMPOSIUM - VOLUME 4. SESSIONS 7, 8A, AND 8B

    EPA Science Inventory

    The report documents more than 100 presentations at the 1993 SO2 Control Symposium in Boston, MA, August 24-27, 1993. The presentations covered a wide range of topics: industry's strategies for dealing with Clean Air Act Amendments of 1990, including Phase I strategies, the emiss...

  5. A putative, novel coli surface antigen 8B (CS8B) of enterotoxigenic Escherichia coli

    PubMed Central

    Njoroge, Samuel M.; Boinett, Christine J.; Madé, Laure F.; Ouko, Tom T.; Fèvre, Eric M.; Thomson, Nicholas R.; Kariuki, Samuel

    2015-01-01

    Enterotoxigenic Escherichia coli (ETEC) strains harbor multiple fimbriae and pili to mediate host colonization, including the type IVb pilus, colonization factor antigen III (CFA/III). Not all colonization factors are well characterized or known in toxin positive ETEC isolates, which may have an impact identifying ETEC isolates based on molecular screening of these biomarkers. We describe a novel coli surface antigen (CS) 8 subtype B (CS8B), a family of CFA/III pilus, in a toxin producing ETEC isolate from a Kenyan collection. In highlighting the existence of this putative CS, we provide the sequence and specific primers, which can be used alongside other ETEC primers previously described. PMID:26187892

  6. A putative, novel coli surface antigen 8B (CS8B) of enterotoxigenic Escherichia coli.

    PubMed

    Njoroge, Samuel M; Boinett, Christine J; Madé, Laure F; Ouko, Tom T; Fèvre, Eric M; Thomson, Nicholas R; Kariuki, Samuel

    2015-10-01

    Enterotoxigenic Escherichia coli (ETEC) strains harbor multiple fimbriae and pili to mediate host colonization, including the type IVb pilus, colonization factor antigen III (CFA/III). Not all colonization factors are well characterized or known in toxin positive ETEC isolates, which may have an impact identifying ETEC isolates based on molecular screening of these biomarkers. We describe a novel coli surface antigen (CS) 8 subtype B (CS8B), a family of CFA/III pilus, in a toxin producing ETEC isolate from a Kenyan collection. In highlighting the existence of this putative CS, we provide the sequence and specific primers, which can be used alongside other ETEC primers previously described. PMID:26187892

  7. A putative, novel coli surface antigen 8B (CS8B) of enterotoxigenic Escherichia coli.

    PubMed

    Njoroge, Samuel M; Boinett, Christine J; Madé, Laure F; Ouko, Tom T; Fèvre, Eric M; Thomson, Nicholas R; Kariuki, Samuel

    2015-10-01

    Enterotoxigenic Escherichia coli (ETEC) strains harbor multiple fimbriae and pili to mediate host colonization, including the type IVb pilus, colonization factor antigen III (CFA/III). Not all colonization factors are well characterized or known in toxin positive ETEC isolates, which may have an impact identifying ETEC isolates based on molecular screening of these biomarkers. We describe a novel coli surface antigen (CS) 8 subtype B (CS8B), a family of CFA/III pilus, in a toxin producing ETEC isolate from a Kenyan collection. In highlighting the existence of this putative CS, we provide the sequence and specific primers, which can be used alongside other ETEC primers previously described.

  8. F-8C adaptive flight control laws

    NASA Technical Reports Server (NTRS)

    Hartmann, G. L.; Harvey, C. A.; Stein, G.; Carlson, D. N.; Hendrick, R. C.

    1977-01-01

    Three candidate digital adaptive control laws were designed for NASA's F-8C digital flyby wire aircraft. Each design used the same control laws but adjusted the gains with a different adaptative algorithm. The three adaptive concepts were: high-gain limit cycle, Liapunov-stable model tracking, and maximum likelihood estimation. Sensors were restricted to conventional inertial instruments (rate gyros and accelerometers) without use of air-data measurements. Performance, growth potential, and computer requirements were used as criteria for selecting the most promising of these candidates for further refinement. The maximum likelihood concept was selected primarily because it offers the greatest potential for identifying several aircraft parameters and hence for improved control performance in future aircraft application. In terms of identification and gain adjustment accuracy, the MLE design is slightly superior to the other two, but this has no significant effects on the control performance achievable with the F-8C aircraft. The maximum likelihood design is recommended for flight test, and several refinements to that design are proposed.

  9. Foothills Parkway Section 8B Final Environmental Report, Volume 4, Appendices E-I

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical, resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER consists of Appendices E through I (all ecological survey reports), which are summarized individually in the sections that follow. The following conclusions result from the completion of these surveys and the ER impact analysis: (1) Forest clearing should be limited as much as possible; (2) Disturbed areas should be replanted with native trees; (3) Drainages should be bridged rather than leveled with cut and fill; (4) For areas of steep slopes and potential erosion

  10. 17 CFR 270.8b-20 - Additional information.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Additional information. 270.8b...) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-20 Additional information. In addition to the information expressly required to be included in a registration statement or report, there...

  11. 15 CFR 8b.20 - Admission and recruitment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Admission and recruitment. 8b.20... Secondary Education § 8b.20 Admission and recruitment. (a) General. Qualified handicapped may not, on the basis of handicap, be denied admission or be subjected to discrimination in admission or recruitment...

  12. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license or certificate obtained from the United States Coast Guard pursuant to the requirements of 46 CFR 10... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Employment on ships. 8b.15 Section...

  13. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license or certificate obtained from the United States Coast Guard pursuant to the requirements of 46 CFR 10... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Employment on ships. 8b.15 Section...

  14. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license or certificate obtained from the United States Coast Guard pursuant to the requirements of 46 CFR 10... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Employment on ships. 8b.15 Section...

  15. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license or certificate obtained from the United States Coast Guard pursuant to the requirements of 46 CFR 10... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Employment on ships. 8b.15 Section...

  16. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license or certificate obtained from the United States Coast Guard pursuant to the requirements of 46 CFR 10... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Employment on ships. 8b.15 Section...

  17. Foothills Parkway Section 8B Final Environmental Report, Volume 1

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west.

  18. 15 CFR 8c.10 - Self-evaluation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Self-evaluation. 8c.10 Section 8c.10... BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF COMMERCE § 8c.10 Self... handicaps or organizations representing individuals with handicaps, to participate in the...

  19. Diminished phosphodiesterase-8B potentiates biphasic insulin response to glucose.

    PubMed

    Dov, Avital; Abramovitch, Eva; Warwar, Nasim; Nesher, Rafael

    2008-02-01

    cAMP activates multiple signal pathways, crucial for the pancreatic beta-cells function and survival and is a major potentiator of insulin release. A family of phosphodiesterases (PDEs) terminate the cAMP signals. We examined the expression of PDEs in rat beta-cells and their role in the regulation of insulin response. Using RT-PCR and Western blot analyses, we identified PDE3A, PDE3B, PDE4B, PDE4D, and PDE8B in rat islets and in INS-1E cells and several possible splice variants of these PDEs. Specific depletion of PDE3A with small interfering (si) RNA (siPDE3A) led to a small (67%) increase in the insulin response to glucose in INS-1E cells but not rat islets. siPDE3A had no effect on the glucagon-like peptide-1 (10 nmol/liter) potentiated insulin response in rat islets. Depletion in PDE8B levels in rat islets using similar technology (siPDE8B) increased insulin response to glucose by 70%, the potentiation being of similar magnitude during the first and second phase insulin release. The siPDE8B-potentiated insulin response was further increased by 23% when glucagon-like peptide-1 was included during the glucose stimulus. In conclusion, PDE8B is expressed in a small number of tissues unrelated to glucose or fat metabolism. We propose that PDE8B, an 3-isobutyl-1-methylxanthine-insensitive cAMP-specific phosphodiesterase, could prove a novel target for enhanced insulin response, affecting a specific pool of cAMP involved in the control of insulin granule trafficking and exocytosis. Finally, we discuss evidence for functional compartmentation of cAMP in pancreatic beta-cells.

  20. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., counseling, financial aid, physical education, athletics, recreation, transportation, other extracurricular, or other post secondary education aid, benefits, or services to which this subpart applies. (b) A... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall,...

  1. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., counseling, financial aid, physical education, athletics, recreation, transportation, other extracurricular, or other post secondary education aid, benefits, or services to which this subpart applies. (b) A... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall,...

  2. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., counseling, financial aid, physical education, athletics, recreation, transportation, other extracurricular, or other post secondary education aid, benefits, or services to which this subpart applies. (b) A... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall,...

  3. 15 CFR 8c.10 - Self-evaluation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Self-evaluation. 8c.10 Section 8c.10...-evaluation. (a) The agency shall, by July 26, 1989, evaluate its current policies and practices, and the... handicaps or organizations representing individuals with handicaps, to participate in the...

  4. 15 CFR 8c.50 - Program accessibility: Existing facilities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF COMMERCE § 8c.50 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in its entirety, is readily accessible to and usable...

  5. WASP-12b AND HAT-P-8b are members of triple star systems

    SciTech Connect

    Bechter, Eric B.; Crepp, Justin R.; Matthews, Christopher T.; Ngo, Henry; Knutson, Heather A.; Batygin, Konstantin; Johnson, John Asher; Hinkley, Sasha; Muirhead, Philip S.; Montet, Benjamin T.; Morton, Timothy D.; Howard, Andrew W.

    2014-06-10

    We present high spatial resolution images that demonstrate that WASP-12b and HAT-P-8b orbit the primary stars of hierarchical triple star systems. In each case, two distant companions with colors and brightnesses consistent with M dwarfs co-orbit the hot Jupiter planet host as well as one another. Our adaptive optics images spatially resolve the secondary around WASP-12, previously identified by Bergfors et al. and Crossfield et al. into two distinct sources separated by 84.3 ± 0.6 mas (21 ± 3 AU). We find that the secondary to HAT-P-8, also identified by Bergfors et al., is in fact composed of two stars separated by 65.3 ± 0.5 mas (15 ± 1 AU). Our follow-up observations demonstrate physical association through common proper motion. HAT-P-8 C has a particularly low mass, which we estimate to be 0.18 ± 0.02 M {sub ☉} using photometry. Due to their hierarchy, WASP-12 BC and HAT-P-8 BC will enable the first dynamical mass determination for hot Jupiter stellar companions. These previously well studied planet hosts now represent higher-order multi-star systems with potentially complex dynamics, underscoring the importance of diffraction-limited imaging and providing additional context for understanding the migrant population of transiting hot Jupiters.

  6. WASP-12b and HAT-P-8b are Members of Triple Star Systems

    NASA Astrophysics Data System (ADS)

    Bechter, Eric B.; Crepp, Justin R.; Ngo, Henry; Knutson, Heather A.; Batygin, Konstantin; Hinkley, Sasha; Muirhead, Philip S.; Johnson, John Asher; Howard, Andrew W.; Montet, Benjamin T.; Matthews, Christopher T.; Morton, Timothy D.

    2014-06-01

    We present high spatial resolution images that demonstrate that WASP-12b and HAT-P-8b orbit the primary stars of hierarchical triple star systems. In each case, two distant companions with colors and brightnesses consistent with M dwarfs co-orbit the hot Jupiter planet host as well as one another. Our adaptive optics images spatially resolve the secondary around WASP-12, previously identified by Bergfors et al. and Crossfield et al. into two distinct sources separated by 84.3 ± 0.6 mas (21 ± 3 AU). We find that the secondary to HAT-P-8, also identified by Bergfors et al., is in fact composed of two stars separated by 65.3 ± 0.5 mas (15 ± 1 AU). Our follow-up observations demonstrate physical association through common proper motion. HAT-P-8 C has a particularly low mass, which we estimate to be 0.18 ± 0.02 M ⊙ using photometry. Due to their hierarchy, WASP-12 BC and HAT-P-8 BC will enable the first dynamical mass determination for hot Jupiter stellar companions. These previously well studied planet hosts now represent higher-order multi-star systems with potentially complex dynamics, underscoring the importance of diffraction-limited imaging and providing additional context for understanding the migrant population of transiting hot Jupiters.

  7. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Ammonium salts of higher fatty acids... Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids...

  8. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 24 2011-07-01 2011-07-01 false Ammonium salts of higher fatty acids... Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids...

  9. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Ammonium salts of higher fatty acids... Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids...

  10. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Ammonium salts of higher fatty acids... Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids...

  11. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Ammonium salts of higher fatty acids... Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids...

  12. 15 CFR 8b.6 - Remedial action, voluntary action, and self-evaluation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... self-evaluation. 8b.6 Section 8b.6 Commerce and Foreign Trade Office of the Secretary of Commerce... DEPARTMENT OF COMMERCE General Provisions § 8b.6 Remedial action, voluntary action, and self-evaluation. (a... or this part and where another recipient exercises control over the recipient that has...

  13. 15 CFR 8b.24 - Financial and employment assistance to students.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... students. 8b.24 Section 8b.24 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF... Secondary Education § 8b.24 Financial and employment assistance to students. (a) Provision of financial... person that provides assistance to any of the recipient's students in a manner that discriminates...

  14. Leucine-rich repeat containing protein LRRC8A is essential for swelling-activated Cl- currents and embryonic development in zebrafish.

    PubMed

    Yamada, Toshiki; Wondergem, Robert; Morrison, Rebecca; Yin, Viravuth P; Strange, Kevin

    2016-10-01

    A volume-regulated anion channel (VRAC) has been electrophysiologically characterized in innumerable mammalian cell types. VRAC is activated by cell swelling and mediates the volume regulatory efflux of Cl(-) and small organic solutes from cells. Two groups recently identified the mammalian leucine-rich repeat containing protein LRRC8A as an essential VRAC component. LRRC8A must be coexpressed with at least one of the other four members of this gene family, LRRC8B-E, to reconstitute VRAC activity in LRRC8(-/-) cells. LRRC8 genes likely arose with the origin of chordates. We identified LRRC8A and LRRC8C-E orthologs in the zebrafish genome and demonstrate that zebrafish embryo cells and differentiated adult cell types express a swelling-activated Cl(-) current indistinguishable from mammalian VRAC currents. Embryo cell VRAC currents are virtually eliminated by morpholino knockdown of the zebrafish LRRC8A ortholog lrrc8aa VRAC activity is fully reconstituted in LRRC8(-/-) human cells by coexpression of zebrafish lrrc8aa and human LRRC8C cDNAs. lrrc8aa expression varies during zebrafish embryogenesis and lrrc8aa knockdown causes pericardial edema and defects in trunk elongation and somatogenesis. Our studies provide confirmation of the importance of LRRC8A in VRAC activity and establish the zebrafish as a model system for characterizing the molecular regulation and physiological roles of VRAC and LRRC8 proteins.

  15. Leucine-rich repeat containing protein LRRC8A is essential for swelling-activated Cl- currents and embryonic development in zebrafish.

    PubMed

    Yamada, Toshiki; Wondergem, Robert; Morrison, Rebecca; Yin, Viravuth P; Strange, Kevin

    2016-10-01

    A volume-regulated anion channel (VRAC) has been electrophysiologically characterized in innumerable mammalian cell types. VRAC is activated by cell swelling and mediates the volume regulatory efflux of Cl(-) and small organic solutes from cells. Two groups recently identified the mammalian leucine-rich repeat containing protein LRRC8A as an essential VRAC component. LRRC8A must be coexpressed with at least one of the other four members of this gene family, LRRC8B-E, to reconstitute VRAC activity in LRRC8(-/-) cells. LRRC8 genes likely arose with the origin of chordates. We identified LRRC8A and LRRC8C-E orthologs in the zebrafish genome and demonstrate that zebrafish embryo cells and differentiated adult cell types express a swelling-activated Cl(-) current indistinguishable from mammalian VRAC currents. Embryo cell VRAC currents are virtually eliminated by morpholino knockdown of the zebrafish LRRC8A ortholog lrrc8aa VRAC activity is fully reconstituted in LRRC8(-/-) human cells by coexpression of zebrafish lrrc8aa and human LRRC8C cDNAs. lrrc8aa expression varies during zebrafish embryogenesis and lrrc8aa knockdown causes pericardial edema and defects in trunk elongation and somatogenesis. Our studies provide confirmation of the importance of LRRC8A in VRAC activity and establish the zebrafish as a model system for characterizing the molecular regulation and physiological roles of VRAC and LRRC8 proteins. PMID:27688432

  16. F-8C digital CCV flight control laws

    NASA Technical Reports Server (NTRS)

    Hartmann, G. L.; Hauge, J. A.; Hendrick, R. C.

    1976-01-01

    A set of digital flight control laws were designed for the NASA F-8C digital fly-by-wire aircraft. The control laws emphasize Control Configured Vehicle (CCV) benefits. Specific pitch axis objectives were improved handling qualities, angle-of-attack limiting, gust alleviation, drag reduction in steady and maneuvering flight, and a capability to fly with reduced static stability. The lateral-directional design objectives were improved Dutch roll damping and turn coordination over a wide range in angle-of-attack. An overall program objective was to explore the use of modern control design methodilogy to achieve these specific CCV benefits. Tests for verifying system integrity, an experimental design for handling qualities evaluation, and recommended flight test investigations were specified.

  17. Global gene profiling of aging lungs in Atp8b1 mutant mice

    PubMed Central

    Soundararajan, Ramani; Stearns, Timothy M.; Czachor, Alexander; Fukumoto, Jutaro; Turn, Christina; Westermann-Clark, Emma; Breitzig, Mason; Tan, Lee; Lockey, Richard F.; King, Benjamin L.; Kolliputi, Narasaiah

    2016-01-01

    Objective Recent studies implicate cardiolipin oxidation in several age-related diseases. Atp8b1 encoding Type 4 P-type ATPases is a cardiolipin transporter. Mutation in Atp8b1 gene or inflammation of the lungs impairs the capacity of Atp8b1 to clear cardiolipin from lung fluid. However, the link between Atp8b1 mutation and age-related gene alteration is unknown. Therefore, we investigated how Atp8b1 mutation alters age-related genes. Methods We performed Affymetrix gene profiling of lungs isolated from young (7-9 wks, n=6) and aged (14 months, 14 M, n=6) C57BL/6 and Atp8b1 mutant mice. In addition, Ingenuity Pathway Analysis (IPA) was performed. Differentially expressed genes were validated by quantitative real-time PCR (qRT-PCR). Results Global transcriptome analysis revealed 532 differentially expressed genes in Atp8b1 lungs, 157 differentially expressed genes in C57BL/6 lungs, and 37 overlapping genes. IPA of age-related genes in Atp8b1 lungs showed enrichment of Xenobiotic metabolism and Nrf2-mediated signaling pathways. The increase in Adamts2 and Mmp13 transcripts in aged Atp8b1 lungs was validated by qRT-PCR. Similarly, the decrease in Col1a1 and increase in Cxcr6 transcripts was confirmed in both Atp8b1 mutant and C57BL/6 lungs. Conclusion Based on transcriptome profiling, our study indicates that Atp8b1 mutant mice may be susceptible to age-related lung diseases. PMID:27689529

  18. 15 CFR 8b.7 - Designation of responsible employee and adoption of grievance procedures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... and adoption of grievance procedures. 8b.7 Section 8b.7 Commerce and Foreign Trade Office of the... adoption of grievance procedures. (a) Designation of responsible employee. A recipient, other than a small...) Adoption of grievance procedures. A recipient, other than a small recipient, shall adopt...

  19. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good... report or any portion thereof may be prepared by any similar process which, in the opinion of...

  20. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good... report or any portion thereof may be prepared by any similar process which, in the opinion of...

  1. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good... portion thereof may be prepared by any similar process which, in the opinion of the Commission,...

  2. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good.... (d) The body of all printed registration statements and reports and all notes to financial...

  3. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... and reports shall be in the English language. If any exhibit or other paper or document filed with a... English language. (f) Where a registration statement or report is distributed through an electronic medium..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES...

  4. The YAV-8B simulation and modeling. Volume 2: Program listing

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Detailed mathematical models of varying complexity representative of the YAV-8B aircraft are defined and documented. These models are used in parameter estimation and in linear analysis computer programs while investigating YAV-8B aircraft handling qualities. Both a six degree of freedom nonlinear model and a linearized three degree of freedom longitudinal and lateral directional model were developed. The nonlinear model is based on the mathematical model used on the MCAIR YAV-8B manned flight simulator. This simulator model has undergone periodic updating based on the results of approximately 360 YAV-8B flights and 8000 hours of wind tunnel testing. Qualified YAV-8B flight test pilots have commented that the handling qualities characteristics of the simulator are quite representative of the real aircraft. These comments are validated herein by comparing data from both static and dynamic flight test maneuvers to the same obtained using the nonlinear program.

  5. Structural mechanism for the regulation of HCN ion channels by the accessory protein TRIP8b

    PubMed Central

    DeBerg, Hannah A.; Bankston, John R.; Rosenbaum, Joel C.; Brzovic, Peter S.; Zagotta, William N.; Stoll, Stefan

    2015-01-01

    Summary Hyperpolarization-activated cyclic nucleotide-gated (HCN) ion channels underlie the cationic Ih current present in many neurons. The direct binding of cAMP to HCN channels increases the rate and extent of channel opening and results in a depolarizing shift in the voltage dependence of activation. TRIP8b is an accessory protein that regulates the cell surface expression and dendritic localization of HCN channels and reduces the cyclic nucleotide dependence of these channels. Here we use electron paramagnetic resonance (EPR) to show that TRIP8b binds to the apo state of the cyclic nucleotide-binding domain (CNBD) of HCN2 channels without changing the overall domain structure. With EPR and nuclear magnetic resonance (NMR), we locate TRIP8b relative to the HCN channel and identify the binding interface on the CNBD. These data provide a structural framework for understanding how TRIP8b regulates the cyclic nucleotide dependence of HCN channels. PMID:25800552

  6. The phospholipid flippase ATP8B1 mediates apical localization of the cystic fibrosis transmembrane regulator.

    PubMed

    van der Mark, Vincent A; de Jonge, Hugo R; Chang, Jung-Chin; Ho-Mok, Kam S; Duijst, Suzanne; Vidović, Dragana; Carlon, Marianne S; Oude Elferink, Ronald P J; Paulusma, Coen C

    2016-09-01

    Progressive familial intrahepatic cholestasis type 1 (PFIC1) is caused by mutations in the gene encoding the phospholipid flippase ATP8B1. Apart from severe cholestatic liver disease, many PFIC1 patients develop extrahepatic symptoms characteristic of cystic fibrosis (CF), such as pulmonary infection, sweat gland dysfunction and failure to thrive. CF is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), a chloride channel essential for epithelial fluid transport. Previously it was shown that CFTR transcript levels were strongly reduced in livers of PFIC1 patients. Here we have investigated the hypothesis that ATP8B1 is important for proper CFTR expression and function. We analyzed CFTR expression in ATP8B1-depleted intestinal and pulmonary epithelial cell lines and assessed CFTR function by measuring short-circuit currents across transwell-grown ATP8B1-depleted intestinal T84 cells and by a genetically-encoded fluorescent chloride sensor. In addition, we studied CFTR surface expression upon induction of CFTR transcription. We show that CFTR protein levels are strongly reduced in the apical membrane of human ATP8B1-depleted intestinal and pulmonary epithelial cell lines, a phenotype that coincided with reduced CFTR activity. Apical membrane insertion upon induction of ectopically-expressed CFTR was strongly impaired in ATP8B1-depleted cells. We conclude that ATP8B1 is essential for correct apical localization of CFTR in human intestinal and pulmonary epithelial cells, and that impaired CFTR localization underlies some of the extrahepatic phenotypes observed in ATP8B1 deficiency. PMID:27301931

  7. BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

    PubMed Central

    Whittle, Andrew J.; Carobbio, Stefania; Martins, Luís; Slawik, Marc; Hondares, Elayne; Vázquez, María Jesús; Morgan, Donald; Csikasz, Robert I.; Gallego, Rosalía; Rodriguez-Cuenca, Sergio; Dale, Martin; Virtue, Samuel; Villarroya, Francesc; Cannon, Barbara; Rahmouni, Kamal; López, Miguel; Vidal-Puig, Antonio

    2012-01-01

    Summary Thermogenesis in brown adipose tissue (BAT) is fundamental to energy balance and is also relevant for humans. Bone morphogenetic proteins (BMPs) regulate adipogenesis, and, here, we describe a role for BMP8B in the direct regulation of thermogenesis. BMP8B is induced by nutritional and thermogenic factors in mature BAT, increasing the response to noradrenaline through enhanced p38MAPK/CREB signaling and increased lipase activity. Bmp8b−/− mice exhibit impaired thermogenesis and reduced metabolic rate, causing weight gain despite hypophagia. BMP8B is also expressed in the hypothalamus, and Bmp8b−/− mice display altered neuropeptide levels and reduced phosphorylation of AMP-activated protein kinase (AMPK), indicating an anorexigenic state. Central BMP8B treatment increased sympathetic activation of BAT, dependent on the status of AMPK in key hypothalamic nuclei. Our results indicate that BMP8B is a thermogenic protein that regulates energy balance in partnership with hypothalamic AMPK. BMP8B may offer a mechanism to specifically increase energy dissipation by BAT. PMID:22579288

  8. Asymptotic normalization coefficients (nuclear vertex constants) for p + 7Be → 8B and the direct 7Be( p, γ)8B astrophysical S factors at solar energies

    NASA Astrophysics Data System (ADS)

    Igamov, S. B.; Yarmukhamedov, R.

    2008-10-01

    A new analysis of the precise experimental astrophysical S factors for the direct-capture reaction 7Be( p, γ)8B [A.J. Junghans et al., Phys. Rev. C 68, 065803 (2003) and L.T. Baby et al., Phys. Rev. C 67, 065805 (2003)] is carried out on the basis of a modified two-body potential approach in which the direct astrophysical S factor, S 17( E), is expressed in terms of the asymptotic normalization constants for p + 7Be → 8B and two additional conditions are involved to verify the peripheral character of the reaction under consideration. The Woods-Saxon potential form is used for the bound-( p + 7Be)-state wave function and for p 7Be-scattering wave function. New estimates are obtained for the “indirectly measured” values of the asymptotic normalization constants (the nuclear vertex constants) for the p + 7Be → 8B and S 17( E) at E ≤ 115 keV, including E = 0. These values of S 17( E) and asymptotic normalization constants have been used for obtaining the indirectly measured values of the s-wave average scattering length and the p-wave effective-range parameters for p 7Be scattering.

  9. First-Principles Study of the Electronic Structure and Bonding Properties of X8C46 and X8B6C40 (X: Li, Na, Mg, Ca) Carbon Clathrates

    NASA Astrophysics Data System (ADS)

    KoleŻyński, Andrzej; Szczypka, Wojciech

    2016-03-01

    Results from theoretical analysis of the crystal structure, electronic structure, and bonding properties of C46 and B6C40 carbon clathrates doped with selected alkali and alkaline earth metals cations (Li, Na, Mg, Ca) are presented. The ab initio calculations were performed by means of the WIEN2k package (full potential linearized augmented plane wave method (FP-LAPW) within density functional theory (DFT)) with PBESol and modified Becke-Johnson exchange-correlation potentials used in geometry optimization and electronic structure calculations, respectively. The bonding properties were analyzed by applying Bader's quantum theory of atoms in molecules formalism to the topological properties of total electron density obtained from ab initio calculations. Analysis of the results obtained (i.a. equilibrium geometry, equation of state, cohesive energy, band structure, density of states—both total and projected on to particular atoms, and topological properties of bond critical points and net charges of topological atoms) is presented in detail.

  10. ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization

    PubMed Central

    Bruurs, Lucas J.M.; Donker, Lisa; Zwakenberg, Susan; Zwartkruis, Fried J.; Begthel, Harry; Knisely, A.S.; Posthuma, George; van de Graaf, Stan F.J.; Paulusma, Coen C.

    2015-01-01

    During yeast cell polarization localization of the small GTPase, cell division control protein 42 homologue (Cdc42) is clustered to ensure the formation of a single bud. Here we show that the disease-associated flippase ATPase class I type 8b member 1 (ATP8B1) enables Cdc42 clustering during enterocyte polarization. Loss of this regulation results in increased apical membrane size with scattered apical recycling endosomes and permits the formation of more than one apical domain, resembling the singularity defect observed in yeast. Mechanistically, we show that to become apically clustered, Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids provided by ATP8B1. Disturbing this interaction, either by ATP8B1 depletion or by introduction of a Cdc42 mutant defective in lipid binding, increases Cdc42 mobility and results in apical membrane enlargement. Re-establishing Cdc42 clustering, by tethering it to the apical membrane or lowering its diffusion, restores normal apical membrane size in ATP8B1-depleted cells. We therefore conclude that singularity regulation by Cdc42 is conserved between yeast and human and that this regulation is required to maintain healthy tissue architecture. PMID:26416959

  11. Autosomal-dominant striatal degeneration is caused by a mutation in the phosphodiesterase 8B gene.

    PubMed

    Appenzeller, Silke; Schirmacher, Anja; Halfter, Hartmut; Bäumer, Sebastian; Pendziwiat, Manuela; Timmerman, Vincent; De Jonghe, Peter; Fekete, Klára; Stögbauer, Florian; Lüdemann, Peter; Hund, Margret; Quabius, Elgar Susanne; Ringelstein, E Bernd; Kuhlenbäumer, Gregor

    2010-01-01

    Autosomal-dominant striatal degeneration (ADSD) is an autosomal-dominant movement disorder affecting the striatal part of the basal ganglia. ADSD is characterized by bradykinesia, dysarthria, and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present. Using genetic linkage analysis, we have mapped the causative genetic defect to a 3.25 megabase candidate region on chromosome 5q13.3-q14.1. A maximum LOD score of 4.1 (Theta = 0) was obtained at marker D5S1962. Here we show that ADSD is caused by a complex frameshift mutation (c.94G>C+c.95delT) in the phosphodiesterase 8B (PDE8B) gene, which results in a loss of enzymatic phosphodiesterase activity. We found that PDE8B is highly expressed in the brain, especially in the putamen, which is affected by ADSD. PDE8B degrades cyclic AMP, a second messenger implied in dopamine signaling. Dopamine is one of the main neurotransmitters involved in movement control and is deficient in Parkinson disease. We believe that the functional analysis of PDE8B will help to further elucidate the pathomechanism of ADSD as well as contribute to a better understanding of movement disorders.

  12. ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization.

    PubMed

    Bruurs, Lucas J M; Donker, Lisa; Zwakenberg, Susan; Zwartkruis, Fried J; Begthel, Harry; Knisely, A S; Posthuma, George; van de Graaf, Stan F J; Paulusma, Coen C; Bos, Johannes L

    2015-09-28

    During yeast cell polarization localization of the small GTPase, cell division control protein 42 homologue (Cdc42) is clustered to ensure the formation of a single bud. Here we show that the disease-associated flippase ATPase class I type 8b member 1 (ATP8B1) enables Cdc42 clustering during enterocyte polarization. Loss of this regulation results in increased apical membrane size with scattered apical recycling endosomes and permits the formation of more than one apical domain, resembling the singularity defect observed in yeast. Mechanistically, we show that to become apically clustered, Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids provided by ATP8B1. Disturbing this interaction, either by ATP8B1 depletion or by introduction of a Cdc42 mutant defective in lipid binding, increases Cdc42 mobility and results in apical membrane enlargement. Re-establishing Cdc42 clustering, by tethering it to the apical membrane or lowering its diffusion, restores normal apical membrane size in ATP8B1-depleted cells. We therefore conclude that singularity regulation by Cdc42 is conserved between yeast and human and that this regulation is required to maintain healthy tissue architecture. PMID:26416959

  13. Autosomal-Dominant Striatal Degeneration Is Caused by a Mutation in the Phosphodiesterase 8B Gene

    PubMed Central

    Appenzeller, Silke; Schirmacher, Anja; Halfter, Hartmut; Bäumer, Sebastian; Pendziwiat, Manuela; Timmerman, Vincent; De Jonghe, Peter; Fekete, Klára; Stögbauer, Florian; Lüdemann, Peter; Hund, Margret; Quabius, Elgar Susanne; Ringelstein, E. Bernd; Kuhlenbäumer, Gregor

    2010-01-01

    Autosomal-dominant striatal degeneration (ADSD) is an autosomal-dominant movement disorder affecting the striatal part of the basal ganglia. ADSD is characterized by bradykinesia, dysarthria, and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present. Using genetic linkage analysis, we have mapped the causative genetic defect to a 3.25 megabase candidate region on chromosome 5q13.3-q14.1. A maximum LOD score of 4.1 (Θ = 0) was obtained at marker D5S1962. Here we show that ADSD is caused by a complex frameshift mutation (c.94G>C+c.95delT) in the phosphodiesterase 8B (PDE8B) gene, which results in a loss of enzymatic phosphodiesterase activity. We found that PDE8B is highly expressed in the brain, especially in the putamen, which is affected by ADSD. PDE8B degrades cyclic AMP, a second messenger implied in dopamine signaling. Dopamine is one of the main neurotransmitters involved in movement control and is deficient in Parkinson disease. We believe that the functional analysis of PDE8B will help to further elucidate the pathomechanism of ADSD as well as contribute to a better understanding of movement disorders. PMID:20085714

  14. Nuclear Structure of 8B Studied by Proton Resonance Scatterings on 7Be

    NASA Astrophysics Data System (ADS)

    Yamaguchi, H.; Wakabayashi, Y.; Hayakawa, S.; Amadio, G.; Kubono, S.; Fujikawa, H.; Saito, A.; He, J. J.; Teranishi, T.; Kwon, Y. K.; Nishimura, S.; Togano, Y.; Iwasa, N.; Inafuku, K.; Niikura, M.; Binh, D. N.; Khiem, L. H.

    2008-05-01

    A new measurement of the proton resonance scattering on 7Be was performed up to the excitation energy of 6.8 MeV using the low-energy RI beam facility CRIB (CNS Radioactive Ion Beam separator) at the Center for Nuclear Study (CNS) of the University of Tokyo. The excitation function of 8B above 3.5 MeV was successfully measured for the first time, providing important information about the reaction rate of 7Be(p,γ)8B, which is the key reaction in the solar 8B neutrino production. For more intensive experimental studies with RI beams, the development of a cryogenic gas target system is ongoing at CNS. In this paper a preliminary result of the 7Be experiment and the present status of the development of the target system are presented.

  15. Phosphodiesterase 8B and cyclic AMP signaling in the adrenal cortex.

    PubMed

    Leal, Leticia Ferro; Szarek, Eva; Faucz, Fabio; Stratakis, Constantine A

    2015-09-01

    Bilateral adrenocortical hyperplasia (BAH) in humans and mice has been recently linked to phosphodiesterase (PDE) 8B (PDE8B) and 11 (PDE11A) defects. These findings have followed the discovery that defects of primary genes of the cyclic monophosphatase (cAMP) signaling pathway, such as guanine nucleotide binding alpha subunit and PRKAR1A, are involved in the pathogenesis of BAH in humans; complete absence of Prkar1a in the adrenal cortex of mice also led to pathology that mimicked the human disease. Here, we review the most recent findings in human and mouse studies on PDE8B, a cAMP-specific PDE that appears to be highly expressed in the adrenal cortex and whose deficiency may underlie predisposition to BAH and possibly other human diseases. PMID:25971952

  16. Nuclear Structure of {sup 8}B Studied by Proton Resonance Scatterings on {sup 7}Be

    SciTech Connect

    Yamaguchi, H.; Wakabayashi, Y.; Hayakawa, S.; Amadio, G.; Kubono, S.; Fujikawa, H.; Niikura, M.; Binh, D. N.; Saito, A.; He, J. J.; Teranishi, T.; Kwon, Y. K.; Nishimura, S.; Togano, Y.; Iwasa, N.; Inafuku, K.; Khiem, L. H.

    2008-05-21

    A new measurement of the proton resonance scattering on {sup 7}Be was performed up to the excitation energy of 6.8 MeV using the low-energy RI beam facility CRIB (CNS Radioactive Ion Beam separator) at the Center for Nuclear Study (CNS) of the University of Tokyo. The excitation function of {sup 8}B above 3.5 MeV was successfully measured for the first time, providing important information about the reaction rate of {sup 7}Be(p,{gamma}){sup 8}B, which is the key reaction in the solar {sup 8}B neutrino production. For more intensive experimental studies with RI beams, the development of a cryogenic gas target system is ongoing at CNS. In this paper a preliminary result of the {sup 7}Be experiment and the present status of the development of the target system are presented.

  17. YAV-8B reaction control system bleed and control power usage in hover and transition

    NASA Technical Reports Server (NTRS)

    Borchers, Paul F.; Moralez, Ernesto, III; Merrick, Vernon K.; Stortz, Michael W.

    1994-01-01

    Using a calibrated Rolls-Royce Pegasus engine and existing aircraft instrumentation and pressure taps, total and individual nozzle reaction control system (RCS) bleed flow rates have been measured on a YAV-8B Harrier during typical short takeoff, transition, hover, and vertical landing maneuvers. RCS thrust forces were calculated from RCS nozzle total pressure measurements, and control power was determined from the moments produced by these thrusts and the aircraft's moments of inertia. These data document the characteristics of the YAV-8B RCS with its basic stability augmentation system (SAS) engaged. Advanced control system designs for the YAV-8B can be compared to the original SAS based on the total bleed use and the percentage of available bleed used. In addition, the peak and mean values of the bleed and control power data can be used for sizing the reaction controls for a future short takeoff and vertical landing (STOVL) aircraft.

  18. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  19. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  20. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  1. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  2. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  3. Analysis of Human TAAR8 and Murine Taar8b Mediated Signaling Pathways and Expression Profile

    PubMed Central

    Mühlhaus, Jessica; Dinter, Juliane; Nürnberg, Daniela; Rehders, Maren; Depke, Maren; Golchert, Janine; Homuth, Georg; Yi, Chun-Xia; Morin, Silke; Köhrle, Josef; Brix, Klaudia; Tschöp, Matthias; Kleinau, Gunnar; Biebermann, Heike

    2014-01-01

    The thyroid hormone derivative 3-iodothyronamine (3-T1AM) exerts metabolic effects in vivo that contradict known effects of thyroid hormones. 3-T1AM acts as a trace amine-associated receptor 1 (TAAR1) agonist and activates Gs signaling in vitro. Interestingly, 3-T1AM-meditated in vivo effects persist in Taar1 knockout-mice indicating that further targets of 3-T1AM might exist. Here, we investigated another member of the TAAR family, the only scarcely studied mouse and human trace-amine-associated receptor 8 (Taar8b, TAAR8). By RT-qPCR and locked-nucleic-acid (LNA) in situ hybridization, Taar8b expression in different mouse tissues was analyzed. Functionally, we characterized TAAR8 and Taar8b with regard to cell surface expression and signaling via different G-protein-mediated pathways. Cell surface expression was verified by ELISA, and cAMP accumulation was quantified by AlphaScreen for detection of Gs and/or Gi/o signaling. Activation of G-proteins Gq/11 and G12/13 was analyzed by reporter gene assays. Expression analyses revealed at most marginal Taar8b expression and no gender differences for almost all analyzed tissues. In heart, LNA-in situ hybridization demonstrated the absence of Taar8b expression. We could not identify 3-T1AM as a ligand for TAAR8 and Taar8b, but both receptors were characterized by a basal Gi/o signaling activity, a so far unknown signaling pathway for TAARs. PMID:25391046

  4. Measurement of the reaction ^7Be(p,γ)^8B with a solid radioactive target

    NASA Astrophysics Data System (ADS)

    Greife, Uwe

    2000-04-01

    The reaction ^7Be(p,γ)^8B is regarded as a key to the understanding of the solar neutrino spectrum and thus most probably to the understanding of new neutrino properties. This contribution describes an experiment measuring the absolute cross section of ^7Be(p,γ)^8B with a solid radioactive target in the energy range between 350 keV and 2.6 MeV. The experiment was performed at the Dynamitron Tandem Laboratory of the Ruhr-Universitaet Bochum, Germany.

  5. Probing the nuclear structure of {sup 8}B and {sup 19}C

    SciTech Connect

    Baumann, T.; Geissel, H.; Aumann, T.; Cortina-Gil, D.; Hellstroem, M.; Muenzenberg, G.; Nickel, F.; Scheidenberger, C.; Schwab, W.; Suemmerer, K.; Winkler, M.; Lenske, H.; Markenroth, K.; Axelsson, L.; Jonson, B.; Nilsson, T.; Smedberg, M. H.; Zhukov, M. V.; Bergmann, U.; Riisager, K.

    1998-12-21

    The nuclei {sup 8}B and {sup 19}C were investigated in breakup reactions at relativistic energies. The fragment separator FRS at GSI was used as an energy-loss spectrometer to measure the longitudinal momentum distributions of the breakup fragments after one-nucleon removal. For the case of {sup 8}B, the measured momentum distribution is in agreement with our previous experiment, but in this measurement the momentum range extends up to 175 MeV/c in the projectile frame. For {sup 18}C fragments from the breakup of {sup 19}C, we extract a much larger momentum width than the value obtained in a measurement at lower projectile energies.

  6. 15 CFR 8b.10 - Effect of state or local law or other requirements and effect of employment opportunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Effect of state or local law or other requirements and effect of employment opportunities. 8b.10 Section 8b.10 Commerce and Foreign Trade Office of... PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE General Provisions § 8b.10 Effect of state or local law...

  7. Trafficking and Gating of Hyperpolarization-activated Cyclic Nucleotide-gated Channels Are Regulated by Interaction with Tetratricopeptide Repeat-containing Rab8b-interacting Protein (TRIP8b) and Cyclic AMP at Distinct Sites*

    PubMed Central

    Han, Ye; Noam, Yoav; Lewis, Alan S.; Gallagher, Johnie J.; Wadman, Wytse J.; Baram, Tallie Z.; Chetkovich, Dane M.

    2011-01-01

    Ion channel trafficking and gating are often influenced by interactions with auxiliary subunits. Tetratricopeptide repeat-containing Rab8b-interacting protein (TRIP8b) is an auxiliary subunit for neuronal hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. TRIP8b interacts directly with two distinct sites of HCN channel pore-forming subunits to control channel trafficking and gating. Here we use mutagenesis combined with electrophysiological studies to define and distinguish the functional importance of the HCN/TRIP8b interaction sites. Interaction with the last three amino acids of the HCN1 C terminus governed the effect of TRIP8b on channel trafficking, whereas TRIP8b interaction with the HCN1 cyclic nucleotide binding domain (CNBD) affected trafficking and gating. Biochemical studies revealed that direct interaction between TRIP8b and the HCN1 CNBD was disrupted by cAMP and that TRIP8b binding to the CNBD required an arginine residue also necessary for cAMP binding. In accord, increasing cAMP levels in cells antagonized the up-regulation of HCN1 channels mediated by a TRIP8b construct binding the CNBD exclusively. These data illustrate the distinct roles of the two TRIP8b-HCN interaction domains and suggest that TRIP8b and cAMP may directly compete for binding the HCN CNBD to control HCN channel gating, kinetics, and trafficking. PMID:21504900

  8. 17 CFR 270.8b-21 - Information unknown or not available.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 17 Commodity and Securities Exchanges 4 2014-04-01 2014-04-01 false Information unknown or not... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-21 Information unknown or not... registrant. If any required information is unknown and not reasonably available to the registrant,...

  9. 17 CFR 270.8b-21 - Information unknown or not available.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 17 Commodity and Securities Exchanges 3 2013-04-01 2013-04-01 false Information unknown or not... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-21 Information unknown or not... registrant. If any required information is unknown and not reasonably available to the registrant,...

  10. 17 CFR 270.8b-21 - Information unknown or not available.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Information unknown or not... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-21 Information unknown or not... registrant. If any required information is unknown and not reasonably available to the registrant,...

  11. 17 CFR 270.8b-21 - Information unknown or not available.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 17 Commodity and Securities Exchanges 3 2012-04-01 2012-04-01 false Information unknown or not... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-21 Information unknown or not... registrant. If any required information is unknown and not reasonably available to the registrant,...

  12. The expansion of Phytophthora clade 8b: three new species associated with winter grown vegetable crops.

    PubMed

    Bertier, L; Brouwer, H; de Cock, A W A M; Cooke, D E L; Olsson, C H B; Höfte, M

    2013-12-01

    Despite its association with important agricultural crops, Phytophthora clade 8b is a poorly studied group of species. The clade currently consists of three officially described species (Phytophthora porri, P. brassicae and P. primulae) that are host-specific pathogens of leek, cabbages and Primula spp., respectively. However, over the past few decades, several other clade 8b-like Phytophthoras have been found on a variety of different host plants that were all grown at low temperatures in winter seasons. In this study, a collection of 30 of these isolates was subjected to a phylogenetic study using two loci (the rDNA ITS region and the mitochondrial cox1 gene). This analysis revealed a clear clustering of isolates according to their host plants. To verify whether these isolates belong to separate species, a detailed morphological study was conducted. On the basis of genetic and morphological differences and host specificity, we now present the official description of three new species in clade 8b: Phytophthora cichorii sp. nov., P. dauci sp. nov. and P. lactucae sp. nov. Two other groups of isolates (Phytophthora taxon castitis and Phytophthora taxon parsley) might also represent new species but the data available at this time are insufficient for an official description. This brings Phytophthora clade 8b to a group of six species that are all host-specific, slow-growing and specifically infect herbaceous crops at low temperatures.

  13. 7Be(p,(gamma))8B S-factor From Ab Initio Wave Functions

    SciTech Connect

    Navratil, P; Bertulani, C; Caurier, E

    2005-08-15

    Nuclear structure of {sup 7}Be, {sup 8}B and {sup 7,8}Li is studied within the ab initio no-core shell model (NCSM). Starting from the high-precision CD-Bonn 2000 nucleon-nucleon (NN) interaction, wave functions of {sup 7}Be and {sup 8}B bound states are obtained in basis spaces up to 10{h_bar}{Omega} and used to calculate channel cluster form factors (overlap integrals) of the {sup 8}B ground state with {sup 7}Be+p. Due to the use of the harmonic oscillator (HO) basis, the overlap integrals have incorrect asymptotic properties. We fix this problem in two alternative ways. First, by a Woods-Saxon (WS) potential solution fit to the interior of the NCSM overlap integrals. Second, by a direct matching with the Whittaker function. The corrected overlap integrals are then used for the {sup 7}Be(p,{gamma}){sup 8}B S-factor calculation. We study the convergence of the S-factor with respect to the NCSM HO frequency and the model space size. Our S-factor results are in agreement with recent direct measurement data.

  14. 17 CFR 270.8b-10 - Requirements as to proper form.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 17 Commodity and Securities Exchanges 4 2014-04-01 2014-04-01 false Requirements as to proper form... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-10 Requirements as to proper form... by the Commission, as in effect on the date of filing. Any such statement or report shall be...

  15. 17 CFR 270.8b-10 - Requirements as to proper form.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Requirements as to proper form... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-10 Requirements as to proper form... by the Commission, as in effect on the date of filing. Any such statement or report shall be...

  16. 17 CFR 270.8b-11 - Number of copies; signatures; binding.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Number of copies; signatures... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-11 Number of copies; signatures; binding. (a) Three complete copies of each registration statement or report, including exhibits and...

  17. Determination of YAV-8B Reaction Control System bleed flow usage

    NASA Technical Reports Server (NTRS)

    Borchers, Paul F.; Moralez, Ernesto, III; Merrick, Vernon K.; Stortz, Michael W.; Eames, David J. H.

    1992-01-01

    Using a calibrated Rolls-Royce Pegasus engine, total Reaction Control System (RCS) bleed flow rates have been measured on a YAV-8B Harrier during typical short takeoff, transition, hover and vertical landing maneuvers. Using existing aircraft instrumentation and pressure taps located in the RCS ducts, bleed flow rates at each RCS valve were also measured directly during flight and ground tests. These data were compared with the calibrated engine data and with the RCS part of a YAV-8B mathematical model used in piloted simulation at NASA Ames Research Center. Areas of disagreement were small, being confined to the estimation of closed RCS valve leakages and the modeling of the RCS butterfly valve pressure losses.

  18. Measurement of the 8B Solar Neutrino Flux with KamLAND

    SciTech Connect

    Abe, S.; Furuno, K.; Gando, A.; Gando, Y.; Ichimura, K.; Ikeda, H.; Inoue, K.; Kibe, Y.; Kimura, W.; Kishimoto, Y.; Koga, M.; Minekawa, Y.; Mitsui, T.; Morikawa, T.; Nagai, N.; Nakajima, K.; Nakamura, K.; Nakamura, M.; Narita, K.; Shimizu, I.; Shimizu, Y.; Shirai, J.; Suekane, F.; Suzuki, A.; Takahashi, H.; Takahashi, N.; Takemoto, Y.; Tamae, K.; Watanabe, H.; Xu, B.D.; Yabumoto, H.; Yonezawa, E.; Yoshida, H.; Yoshida, S.; Enomoto, S.; Kozlov, A.; Murayama, H.; Grant, C.; Keefer, G.; McKee, D.; Piepke, A.; Banks, T.I.; Bloxham, T.; Detwiler, J.A.; Freedman, S.J.; Fujikawa, B.K.; Han, K.; Kadel, R.; O'Donnell, T.; Steiner, H.M.; Winslow, L.A.; Dwyer, D.A.; Mauger, C.; McKeown, R.D.; Zhang, C.; Berger, B.E.; Lane, C.E.; Maricic, J.; Miletic, T.; Batygov, M.; Learned, J.G.; Matsuno, S.; Pakvasa, S.; Sakai, M.; Horton-Smith, G.A.; Tang, A.; Downum, K.E.; Gratta, G.; Tolich, K.; Efremenko, Y.; Kamyshkov, Y.; Perevozchikov, O.; Karwowski, H.J.; Markoff, D.M.; Tornow, W.; Heeger, K.M.; Piquemal, F.; Ricol, J.-S.; Decowski, M.P.

    2011-06-04

    We report a measurement of the neutrino-electron elastic scattering rate from {sup 8}B solar neutrinos based on a 123 kton-day exposure of KamLAND. The background-subtracted electron recoil rate, above a 5.5-MeV analysis threshold is 1.49 {+-} 0.14(stat) {+-} 0.17(syst) events per kton-day. Interpreted as due to a pure electron flavor flux with a {sup 8}B neutrino spectrum, this corresponds to a spectrum integrated flux of 2.77 {+-} 0.26(stat) {+-} 0.32(syst) x 10{sup 6} cm{sup -2}s{sup -1}. The analysis threshold is driven by {sup 208}Tl present in the liquid scintillator, and the main source of systematic uncertainty is due to background from cosmogenic {sup 11}Be. The measured rate is consistent with existing measurements and with standard solar model predictions which include matter-enhanced neutrino oscillation.

  19. Complete Genome Sequence of Fowl Aviadenovirus Serotype 8b Isolated in South America

    PubMed Central

    Calderón, Katherine; Chumbe, Ana; Montesinos, Ricardo; Montalván, Ángela; González, Armando E.; Icochea, Eliana; Fernández-Díaz, Manolo

    2016-01-01

    We present here the complete genome sequence of fowl aviadenovirus E (FAdV-E) serotype 8b strain FV211-16, isolated from chickens with inclusion body hepatitis in Peru. Genome comparisons with other FAdV-E strains revealed identities of 84.9 to 97.1% and the presence of 9 and 2 unique amino acid mutations in hexon and fiber proteins, respectively.

  20. A lower radius and mass for the transiting extrasolar planet HAT-P-8 b

    NASA Astrophysics Data System (ADS)

    Mancini, L.; Southworth, J.; Ciceri, S.; Fortney, J. J.; Morley, C. V.; Dittmann, J. A.; Tregloan-Reed, J.; Bruni, I.; Barbieri, M.; Evans, D. F.; D'Ago, G.; Nikolov, N.; Henning, Th.

    2013-03-01

    Context. The extrasolar planet HAT-P-8 b was thought to be one of the more inflated transiting hot Jupiters. Aims: By using new and existing photometric data, we computed precise estimates of the physical properties of the system. Methods: We present photometric observations comprising eleven light curves covering six transit events, obtained using five medium-class telescopes and telescope-defocussing technique. One transit was simultaneously obtained through four optical filters, and two transits were followed contemporaneously from two observatories. We modelled these and seven published datasets using the jktebop code. The physical parameters of the system were obtained from these results and from published spectroscopic measurements. In addition, we investigated the theoretically-predicted variation of the apparent planetary radius as a function of wavelength, covering the range 330-960 nm. Results: We find that HAT-P-8 b has a significantly lower radius (1.321 ± 0.037 RJup) and mass (1.275 ± 0.053 MJup) compared to previous estimates (1.50-0.06+0.08 R_{Jup} and 1.52-0.16+0.18 M_{Jup} respectively). We also detect a radius variation in the optical bands that, when compared with synthetic spectra of the planet, may indicate the presence of a strong optical absorber, perhaps TiO and VO gases, near the terminator of HAT-P-8 b. Conclusions: These new results imply that HAT-P-8 b is not significantly inflated, and that its position in the planetary mass-radius diagram is congruent with those of many other transiting extrasolar planets. Full Table 2 is only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/551/A11

  1. Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes.

    PubMed

    Kobayashi, Shintaro; Suzuki, Tadaki; Kawaguchi, Akira; Phongphaew, Wallaya; Yoshii, Kentaro; Iwano, Tomohiko; Harada, Akihiro; Kariwa, Hiroaki; Orba, Yasuko; Sawa, Hirofumi

    2016-03-18

    West Nile virus (WNV) particles assemble at and bud into the endoplasmic reticulum (ER) and are secreted from infected cells through the secretory pathway. However, the host factor related to these steps is not fully understood. Rab proteins, belonging to the Ras superfamily, play essential roles in regulating many aspects of vesicular trafficking. In this study, we sought to determine which Rab proteins are involved in intracellular trafficking of nascent WNV particles. RNAi analysis revealed that Rab8b plays a role in WNV particle release. We found that Rab8 and WNV antigen were colocalized in WNV-infected human neuroblastoma cells, and that WNV infection enhanced Rab8 expression in the cells. In addition, the amount of WNV particles in the supernatant of Rab8b-deficient cells was significantly decreased compared with that of wild-type cells. We also demonstrated that WNV particles accumulated in the recycling endosomes in WNV-infected cells. In summary, these results suggest that Rab8b is involved in trafficking of WNV particles from recycling endosomes to the plasma membrane. PMID:26817838

  2. Evolution of substellar 'brown' dwarfs and the evolutionary status of VB8B

    NASA Technical Reports Server (NTRS)

    Stringfellow, Guy S.

    1986-01-01

    Detailed evolutionary calculations for stars near and below the minimum main sequence (MS) mass are presented, and the results are compared to VB8B. The standard set of stellar equations are solved, and the influence of both high and low grain opacities is assessed. All masses less than 0.08 solar are found to evolve to 0.0001 solar luminosities in a billion years or less. Both 0.08 solar mass and 0.075 solar mass stars become MS stars after five billion years, but with luminosities substantially below 0.0001 solar. The best fit to VB8B is 0.06 + or - 0.02 solar mass, but less than 0.8 solar mass. Depending upon its very precise mass, VB8B can be either a substellar brown dwarf with an age of 0.3-3 billion years, or an MS star with an age anywhere from two billion years to a Hubble time.

  3. The Characterization of the Cool and Eccentric Exoplanet WASP-8b with Spitzer

    NASA Astrophysics Data System (ADS)

    Cubillos, Patricio; Harrington, J.; Madhusudhan, N.; Stevenson, K.; Hardy, R.; Blecic, J.; Anderson, D.; Hardin, M.; Campo, C.

    2012-10-01

    WASP-8b is one of the coldest hot-Jupiter planets observed during secondary eclipse (when the planet passes behind the star) with the Spitzer Space Telescope. We present the our observations of WASP-8b at the 3.6, 4.5, and 8.0-micron wavebands with the Spitzer's IRAC instrument, during secondary eclipse. We will show the resulting light curves of our infrared observations, determining the planet-to-star flux ratios. With this spectral information we further characterized the planet's dayside atmosphere, constraining its chemical composition, recognizing the absence of a thermal inversion, and estimating the energy redistribution regime over its surface. Although having a equilibrium temperature is only 950K, the large eccentricity of the orbit (e=0.31) should make the dayside temperature of WASP-8b oscillate with an amplitude of hundreds of degrees. We modeled these temporal variation of the temperature over the surface of the planet and set constrains on the rotational angular velocity and radiative timescale of the planet, based in the observed hemisphere-averaged brightness temperature during secondary eclipse. This planet is also dynamically interesting since it orbits the primary star of a binary system. Along with a large eccentricity, suggests the presence of an unseen planetary companion. A precise determination of the eclipse mid-times will help to constrain the orbit of such companion.

  4. Structural and electronic properties of neutral and charged Ca 8C 12 metal carbides

    NASA Astrophysics Data System (ADS)

    Chen, Gang; Peng, Qi; Kawazoe, Yoshiyuki

    2011-05-01

    This Letter reports our detailed first-principles study on neutral and charged Ca8C12 clusters. Except a compact structure owning geometrical characters of the CaC2 bulk, all the other low-lying structures within 0.2 eV in relative total energy are hollow configurations. Most of the low-lying structures are calculated to be magnetic. The ground states are a D3d flat structure, a compact structure, and a D3d hollow cage-like structure for Ca8C12, Ca8C12-, and Ca8C12+ clusters, respectively. The net charge on Ca ion, except the dangling one of the compact structure, is calculated to be around +1.4e.

  5. High-Temperature Performance of Cast CF8C-Plus Austenitic Stainless Steel

    SciTech Connect

    Maziasz, Philip J; Pint, Bruce A

    2011-01-01

    Covers and casings of small to medium size gas turbines can be made from cast austenitic stainless steels, including grades such as CF8C, CF3M, or CF10M. Oak Ridge National Laboratory and Caterpillar have developed a new cast austenitic stainless steel, CF8C-Plus, which is a fully austenitic stainless steel, based on additions of Mn and N to the standard Nb-stabilized CF8C steel grade. The Mn addition improves castability, as well as increases the alloy solubility for N, and both Mn and N synergistically act to boost mechanical properties. CF8C-Plus steel has outstanding creep-resistance at 600-900 C, which compares well with Ni-based superalloys such as alloys X, 625, 617, and 230. CF8C-Plus also has very good fatigue and thermal fatigue resistance. It is used in the as-cast condition, with no additional heat-treatments. While commercial success for CF8C-Plus has been mainly for diesel exhaust components, this steel can also be considered for gas turbine and microturbine casings. The purposes of this paper are to demonstrate some of the mechanical properties, to update the long-term creep-rupture data, and to present new data on the high-temperature oxidation behavior of these materials, particularly in the presence of water vapor.

  6. Conserved POU/OCT- and GATA-binding sites in 5'-flanking promoter region of mammalian WNT8B orthologs.

    PubMed

    Katoh, Masuko; Katoh, Masaru

    2007-05-01

    WNT family members are secreted-type glycoproteins regulating cell fate, planar cell polarity, cell adhesion, and cell movement. WNT signals are context-dependently transduced to the canonical pathway for the transcriptional up-regulation of MYC, CCND1, FGF20, JAG1, WISP1 and DKK1 genes, and also to the non-canonical pathway for the activation of RHOA, JNK, PKC, NFAT and NLK signaling cascades. We cloned and characterized the wild-type human WNT8B, while another group the aberrant human WNT8B with Gly230Ala and Arg284Leu amino-acid substitutions. Although WNT8B is undetectable in normal adult tissues by using Northern blot analyses, WNT8B is expressed in gastric cancer, pancreatic cancer, colorectal cancer, breast cancer, and embryonal tumors. Here, comparative integromics on WNT8B orthologs were investigated by using bioinformatics (Techint) and human intelligence (Humint). Cow Wnt8b gene was identified within NW_001494361.1 genome sequence. Predicted sequence XM_582222.3 was an artificial cow Wnt8b with aberrant prediction for the first exon. Cow Wnt8b complete coding sequence was found to encode a 350-amino-acid protein, which showed 96.9% total-amino-acid identity with human WNT8B. Comparative proteomics revealed that N-terminal signal peptide, 22 Cys residues, two Asn-linked glycosylation sites, Gly230, and Arg284 of human WNT8B were conserved among mammalian WNT8B orthologs. Comparative genomics revealed that POU/OCT- and GATA-binding sites in the 5'-flanking promoter region were conserved among human, chimpanzee, cow, mouse, and rat WNT8B orthologs. In silico expression analyses revealed that human WNT8B was expressed in embryoid body derived from embryonic stem (ES) cells, hepatocyte progenitors derived from ES cells, fetal brain, diffuse-type gastric cancer, colorectal cancer, prostate cancer, and ovarian fibrotheoma. Based on the expression profiles of POU and GATA family transcription factors, it was revealed that WNT8B expression in hepatocyte

  7. DETECTING GRAVITY MODES IN THE SOLAR {sup 8} B NEUTRINO FLUX

    SciTech Connect

    Lopes, Ilídio; Turck-Chièze, Sylvaine E-mail: ilopes@uevora.pt

    2014-09-10

    The detection of gravity modes produced in the solar radiative zone has been a challenge in modern astrophysics for more than 30 yr and their amplitude in the core is not yet determined. In this Letter, we develop a new strategy to look for standing gravity modes through solar neutrino fluxes. We note that due to a resonance effect, the gravity modes of low degree and low order have the largest impact on the {sup 8} B neutrino flux. The strongest effect is expected to occur for the dipole mode with radial order 2, corresponding to periods of about 1.5 hr. These standing gravity waves produce temperature fluctuations that are amplified by a factor of 170 in the boron neutrino flux for the corresponding period, in consonance with the gravity modes. From current neutrino observations, we determine that the maximum temperature variation due to the gravity modes in the Sun's core is smaller than 5.8 × 10{sup –4}. This study clearly shows that due to their high sensitivity to the temperature, the {sup 8} B neutrino flux time series is an excellent tool to determine the properties of gravity modes in the solar core. Moreover, if gravity mode footprints are discovered in the {sup 8} B neutrino flux, this opens a new line of research to probe the physics of the solar core as non-standing gravity waves of higher periods cannot be directly detected by helioseismology but could leave their signature on boron neutrino or on other neutrino fluxes.

  8. An 8-b 1.3-MHz successive-approximation A/D converter

    NASA Technical Reports Server (NTRS)

    Hadidi, KH.; Tso, Vincent S.; Temes, Gabor C.

    1990-01-01

    A new successive-approximation A/D converter is described. It combines a string of equal-valued polysilicon resistors and a set of ratioed capacitors in a unique circuit configuration so that a high sampling rate is achieved. The comparator is realized by a chopper-stabilized amplifier to reduce the effect of the offset voltages of MOS amplifiers. The converter performs an 8-b monotonic conversion with a differential nonlinearity less than 1 LSB in 770 nsec. The die area is 3750 sq mil. This new conversion technique can also be utilized in a pipelined A/D converter (Temes, 1985) and enables it to achieve high speed.

  9. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... citations affecting Form N-8B-3, see the List of CFR Sections Affected, which appears in the Finding Aids... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.13 Form N..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  10. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... citations affecting Form N-8B-3, see the List of CFR Sections Affected, which appears in the Finding Aids... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.13 Form N..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  11. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... citations affecting Form N-8B-3, see the List of CFR Sections Affected, which appears in the Finding Aids... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.13 Form N..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  12. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... citations affecting Form N-8B-3, see the List of CFR Sections Affected, which appears in the Finding Aids... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.13 Form N..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  13. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... citations affecting Form N-8B-3, see the List of CFR Sections Affected, which appears in the Finding Aids... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.13 Form N..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  14. A long pentraxin-3-derived pentapeptide for the therapy of FGF8b-driven steroid hormone-regulated cancers.

    PubMed

    Giacomini, Arianna; Matarazzo, Sara; Pagano, Katiuscia; Ragona, Laura; Rezzola, Sara; Corsini, Michela; Di Salle, Emanuela; Presta, Marco; Ronca, Roberto

    2015-05-30

    Fibroblast growth factor-8b (FGF8b) affects the epithelial/stromal compartments of steroid hormone-regulated tumors by exerting an autocrine activity on cancer cells and a paracrine pro-angiogenic function, thus contributing to tumor progression. The FGF8b/FGF receptor (FGFR) system may therefore represent a target for the treatment of steroid hormone-regulated tumors. The soluble pattern recognition receptor long pentraxin-3 (PTX3) binds various FGFs, including FGF2 and FGF8b, thus inhibiting the angiogenic and tumorigenic activity of androgen-regulated tumor cells. Nevertheless, the complex/proteinaceous structure of PTX3 hampers its pharmacological exploitation. In this context, the acetylated pentapeptide Ac-ARPCA-NH2 (ARPCA), corresponding to the N-terminal amino acid sequence PTX3(100-104), was identified as a minimal FGF2-binding peptide able to antagonize the biological activity of FGF2. Here, we demonstrate that ARPCA binds FGF8b and inhibits its capacity to form FGFR1-mediated ternary complexes with heparan sulphate proteoglycans. As a FGF8b antagonist, ARPCA inhibits FGFR1 activation and signalling in endothelial cells, hampering the angiogenic activity exerted in vitro and in vivo by FGF8b. Also, ARPCA suppresses the angiogenic and tumorigenic potential of prototypic androgen/FGF8b-dependent Shionogi 115 mammary carcinoma cells and of androgen/FGF8b/FGF2-dependent TRAMP-C2 prostate cancer cells. In conclusion, ARPCA represents a novel FGF8b antagonist with translational implications for the therapy of steroid hormone-regulated tumors.

  15. Adsorption of Procion Red MX 8B using spent tea leaves as adsorbent

    NASA Astrophysics Data System (ADS)

    Heraldy, Eddy; Osa, Riesta Ramdhaniyati; Suryanti, Venty

    2016-02-01

    The adsorption of Procion Red MX 8B using spent tea leaves (STL) as adsorbent, has been studied by batch adsorption technique. The adsorbent was activated by NaOH 4% for 24 hours for delignification process. The adsorbent was characterized using FTIR to indetify the functional groups of cellulose was shown by uptake -OH, C-H and C-O. The optimum conditions of adsorption experiments were achieved when pH was set as 6 with contact time of 75 minutes and capacity of adsorption was 3.28 mg/g. The equilibrium data were fitted to Langmuir and Isotherm Freundlichs. The kinetic models, pseudo first order and pseudo second order were employed to describe the adsorption mechanism. The experimental results showed that the pseudo second order equation was the best model that described the adsorption behavior with the coefficient of correlation (R2) was equal higher than 0.99 The results suggested that STL had high potential to be used as effective adsorbent for Procion Red MX 8B removal.

  16. WASP-8b: CHARACTERIZATION OF A COOL AND ECCENTRIC EXOPLANET WITH SPITZER

    SciTech Connect

    Cubillos, Patricio; Harrington, Joseph; Hardy, Ryan A.; Blecic, Jasmina; Hardin, Matthew; Campo, Christopher J.; Madhusudhan, Nikku; Stevenson, Kevin B.; Anderson, David R.

    2013-05-01

    WASP-8b has 2.18 times Jupiter's mass and is on an eccentric (e = 0.31) 8.16 day orbit. With a time-averaged equilibrium temperature of 948 K, it is one of the least-irradiated hot Jupiters observed with the Spitzer Space Telescope. We have analyzed six photometric light curves of WASP-8b during secondary eclipse observed in the 3.6, 4.5, and 8.0 {mu}m Infrared Array Camera bands. The eclipse depths are 0.113% {+-} 0.018%, 0.069% {+-} 0.007%, and 0.093% {+-} 0.023%, respectively, giving respective brightness temperatures of 1552, 1131, and 938 K. We characterized the atmospheric thermal profile and composition of the planet using a line-by-line radiative transfer code and a Markov-chain Monte Carlo sampler. The data indicated no thermal inversion, independently of any assumption about chemical composition. We noted an anomalously high 3.6 {mu}m brightness temperature (1552 K); by modeling the eccentricity-caused thermal variation, we found that this temperature is plausible for radiative timescales less than {approx}10{sup 2} hr. However, as no model spectra fit all three data points well, the temperature discrepancy remains as an open question.

  17. 7Be(p,gamma)8B S-factor from Ab Initio Wave Functions

    SciTech Connect

    Navratil, P; Bertulani, C A; Caurier, E

    2006-10-12

    There has been a significant progress in ab initio approaches to the structure of light nuclei. Starting from realistic two- and three-nucleon interactions the ab initio no-core shell model (NCSM) predicts low-lying levels in p-shell nuclei. It is a challenging task to extend ab initio methods to describe nuclear reactions. We present here a brief overview of the first steps taken toward nuclear reaction applications. In particular, we discuss our calculation of the {sup 7}Be(p,{gamma}){sup 8}B S-factor. We also present our first results of the {sup 3}He({alpha},{gamma}){sup 7}Be S-factor and of the S-factor of the mirror reaction {sup 3}H({alpha},{gamma}){sup 7}Li. The {sup 7}Be(p,{gamma}){sup 8}B and {sup 3}He({alpha},{gamma}){sup 7}Be reactions correspond to the most important uncertainties in solar model predictions of neutrino fluxes.

  18. WASP-8b: Characterization of a Cool and Eccentric Exoplanet with Spitzer

    NASA Astrophysics Data System (ADS)

    Cubillos, Patricio; Harrington, Joseph; Madhusudhan, Nikku; Stevenson, Kevin B.; Hardy, Ryan A.; Blecic, Jasmina; Anderson, David R.; Hardin, Matthew; Campo, Christopher J.

    2013-05-01

    WASP-8b has 2.18 times Jupiter's mass and is on an eccentric (e = 0.31) 8.16 day orbit. With a time-averaged equilibrium temperature of 948 K, it is one of the least-irradiated hot Jupiters observed with the Spitzer Space Telescope. We have analyzed six photometric light curves of WASP-8b during secondary eclipse observed in the 3.6, 4.5, and 8.0 μm Infrared Array Camera bands. The eclipse depths are 0.113% ± 0.018%, 0.069% ± 0.007%, and 0.093% ± 0.023%, respectively, giving respective brightness temperatures of 1552, 1131, and 938 K. We characterized the atmospheric thermal profile and composition of the planet using a line-by-line radiative transfer code and a Markov-chain Monte Carlo sampler. The data indicated no thermal inversion, independently of any assumption about chemical composition. We noted an anomalously high 3.6 μm brightness temperature (1552 K) by modeling the eccentricity-caused thermal variation, we found that this temperature is plausible for radiative timescales less than ~102 hr. However, as no model spectra fit all three data points well, the temperature discrepancy remains as an open question.

  19. Cast CF8C-Plus Stainless Steel for Turbocharger Applications

    SciTech Connect

    Maziasz, P.J.; Shyam, A.; Evans, N.D.; Pattabiraman, K. (Honeywell Turbo Technologies

    2010-06-30

    The purpose of this Cooperative Research and Development Agreement (CRADA) project is to provide the critical test data needed to qualify CF8C-Plus cast stainless steel for commercial production and use for turbocharger housings with upgraded performance and durability relative to standard commercial cast irons or stainless steels. The turbocharger technologies include, but are not limited to, heavy-duty highway diesel engines, and passenger vehicle diesel and gasoline engines. This CRADA provides additional critical high-temperature mechanical properties testing and data analysis needed to quality the new CF8C-Plus steels for turbocharger housing applications.

  20. Flight test and numerical simulation of transonic flow around YAV-8B Harrier II wing

    NASA Technical Reports Server (NTRS)

    Gea, Lie-Mine; Chyu, Wei J.; Stortz, Michael W.; Roberts, Andrew C.; Chow, Chuen-Yen

    1991-01-01

    A computational fluid dynamics (CFD) method is used to study the aerodynamics of the YAV-8B Harrier II wing in the transonic region. A numerical procedure is developed to compute the flow field around the complicated wing-pylon-fairing geometry. The surface definition of the wing and pylons were obtained from direct measurement using theodolite triangulation. A thin-layer Navier-Stokes code with the Chimera technique is used to compute flow solutions. The computed pressure distributions at several span stations are compared with flight test data and show good agreement. Computed results are correlated with flight test data that show the flow is severely separated in the vicinity of the wing-pylon junction. Analysis shows that shock waves are induced by pylon swaybrace fairings, that the flow separation is much stronger at the outboard pylon and that the separation is caused mainly by the crossflow passing the geometry of wing-pylon junction.

  1. Searches for high frequency variations in the 8-B neutrino flux at the Sudbury neutrino observatory

    SciTech Connect

    Rielage, Keith; Seibert, Stanley R; Hime, Andrew; Elliott, Steven R; Stonehill, L C; Wouters, J M; Aharmim, B; Ahmed, S N; Anthony, A E; Barros, N; Beier, E W; Bellerive, A; Belttran, B; Bergevin, M; Biller, S D; Boudjemline, K; Burritt, T H; Cai, B; Chan, Y D; Chauhan, D; Chen, M; Cleveland, B T; Cox - Mobrand, G A; Dai, X; Deng, H; Detwiler, J; Dimarco, M; Doe, P J; Drouin, P - L; Duba, C A; Duncan, F A; Dunford, M; Earle, E D; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Ford, R J; Formaggilo, J A; Gagnon, N; Goon, J Tm; Guillian, E; Habib, S; Hahn, R L; Hallin, A L; Hallman, E D; Harvey, P J; Hazama, R; Heintzelman, W J; Heise, J; Helmer, R L; Howard, C; Howe, M A; Huang, M; Jamieson, B; Jelley, N A; Keeter, K J; Klein, J R; Kos, M; Kraus, C; Krauss, C B; Kutter, T; Kyba, C C M; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Loach, J C; Maclellan, R; Majerus, S; Mak, H B; Maneira, J; Martin, R; Mccauley, N; Mc Donald, A B; Mcgee, S; Miffin, C; Miller, M L; Monreal, B; Monroe, J; Morissette, B; Nickel, B G; Noble, A J; O' Keeffe, H M; Oblath, N S; Orebi Gann, G D; Oser, S M; Ott, R A; Peeters, S J M; Poon, A W P; Prior, G; Reitzner, S D; Robertson, B C; Robertson, R G H; Rollin, E; Schwendener, M H; Secrest, J A; Seibert, S R; Simard, O; Sinclair, D; Sinclair, L; Skensved, P; Sonley, T J; Tesic, G; Tolich, N; Tsui, T; Tunnell, C D; Van Berg, R; Van Devender, B A; Virtue, C J; Wall, B L; Waller, D; Wan Chan Tseung, H; West, N; Wilkerson, J F; Wilson, J R; Wright, A; Yeh, M; Zhang, F; Zuber, K

    2009-01-01

    We have peformed three searches for high-frequency signals in the solar neutrino flux measured by the Sudbury Neutrino Observatory (SNO), motivated by the possibility that solar g-mode oscillations could affect the production or propagation of solar {sup 8}B neutrinos. The first search looked for any significant peak in the frequency range l/day to 144/day, with a sensitivity to sinusoidal signals with amplitudes of 12% or greater. The second search focused on regions in which g-mode signals have been claimed by experiments aboard the SoHO satellite, and was sensitive to signals with amplitudes of 10% or greater. The third search looked for extra power across the entire frequency band. No statistically significant signal was detected in any of the three searches.

  2. 1995 Bird survey Foothills parkway section 8B National Park Service, Tennessee

    SciTech Connect

    Wade, M.C.; Giffen, N.R.; Wade, B.A.

    1996-05-01

    The Foothills Parkway Section 8B right-of-way (ROW) is a stretch of land between Pittman Center and Cosby, Tennessee that is approximately 14.2 miles long and 1,000 ft wide, with a considerably wider section on Webb Mountain. A breeding bird survey was conducted at selected sample points along the ROW. The intent of the survey was to identify bird communities, area sensitive species (birds dependent on extensive forest systems for all their needs) and endangered, threatened, federal candidate, and state `in need of management` species now using the ROW. The survey also provides baseline data to assess future habitat impacts as well as cumulative impacts of the project.

  3. WASP-8b: a retrograde transiting planet in a multiple system

    NASA Astrophysics Data System (ADS)

    Queloz, D.; Anderson, D. R.; Collier Cameron, A.; Gillon, M.; Hebb, L.; Hellier, C.; Maxted, P.; Pepe, F.; Pollacco, D.; Ségransan, D.; Smalley, B.; Triaud, A. H. M. J.; Udry, S.; West, R.

    2010-07-01

    We report the discovery of WASP-8b, a transiting planet of 2.25 ± 0.08 MJup on a strongly inclined eccentric 8.15-day orbit, moving in a retrograde direction to the rotation of its late-G host star. Evidence is found that the star is in a multiple stellar system with two other companions. The dynamical complexity of the system indicates that it may have experienced secular interactions such as the Kozai mechanism or a formation that differs from the “classical” disc-migration theory. Based on observations made with HARPS spectrograph on the 3.6-m ESO telescope and the EULER Swiss telescope at La Silla Observatory, Chile.Radial velocity data are only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/cgi-bin/qcat?J/A+A/517/L1

  4. The Coulomb Dissociation of {sup 8}B: A New Tool in Nuclear Astrophysics

    SciTech Connect

    Gai, Moshe

    2008-01-24

    The GSI1, GSI2 (as well as the RIKEN2 and the corrected GSI2) measurements of the Coulomb Dissociation (CD) of {sup 8}B are in good agreement with the most recent Direct Capture (DC) {sup 7}Be(p,{gamma}){sup 8}B reaction measurement performed at Weizmann and in agreement with the Seattle result. Yet it was claimed that the CD and DC results are sufficiently different and need to be reconciled. We show that these statements arise from a misunderstanding (as well as misrepresentation) of CD experiments. We recall a similar strong statement questioning the validity of the CD method due to an invoked large E2 component that was also shown to arise from a misunderstanding of the CD method. In spite of the good agreement between DC and CD data the slope of the astrophysical cross section factor (S{sub 17}) can not be extracted with high accuracy due to discrepancies among the most recent DC data as well as a discrepancies among the three reports of the GSI CD data. The slope is directly related to the d-wave component that dominates at higher energies. This d-wave component must be subtracted from measured data to extrapolate to zero energy. Hence the uncertainty of the measured slope leads to an additional downward uncertainty ({sub -3.0}{sup +0.0} eV-b) of the extrapolated zero energy cross section factor, S{sub 17}(0). Such an uncertainty is also consistent with the smaller value of S{sub 17}(0) extracted using the ANC method. This uncertainty must be alleviated by future experiments to allow a precise determination of S{sub 17}(0), a goal that so far has not be achieved in spite of strong statement(s) that appeared in the literature.

  5. Structure and stoichiometry of an accessory subunit TRIP8b interaction with hyperpolarization-activated cyclic nucleotide-gated channels

    PubMed Central

    Bankston, John R.; Camp, Stacey S.; DiMaio, Frank; Lewis, Alan S.; Chetkovich, Dane M.; Zagotta, William N.

    2012-01-01

    Ion channels operate in intact tissues as part of large macromolecular complexes that can include cytoskeletal proteins, scaffolding proteins, signaling molecules, and a litany of other molecules. The proteins that make up these complexes can influence the trafficking, localization, and biophysical properties of the channel. TRIP8b (tetratricopetide repeat-containing Rab8b-interacting protein) is a recently discovered accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels that contributes to the substantial dendritic localization of HCN channels in many types of neurons. TRIP8b interacts with the carboxyl-terminal region of HCN channels and regulates their cell-surface expression level and cyclic nucleotide dependence. Here we examine the molecular determinants of TRIP8b binding to HCN2 channels. Using a single-molecule fluorescence bleaching method, we found that TRIP8b and HCN2 form an obligate 4:4 complex in intact channels. Fluorescence-detection size-exclusion chromatography and fluorescence anisotropy allowed us to confirm that two different domains in the carboxyl-terminal portion of TRIP8b—the tetratricopepide repeat region and the TRIP8b conserved region—interact with two different regions of the HCN carboxyl-terminal region: the carboxyl-terminal three amino acids (SNL) and the cyclic nucleotide-binding domain, respectively. And finally, using X-ray crystallography, we determined the atomic structure of the tetratricopepide region of TRIP8b in complex with a peptide of the carboxy-terminus of HCN2. Together, these experiments begin to uncover the mechanism for TRIP8b binding and regulation of HCN channels. PMID:22550182

  6. Performance of aluminide coatings applied on alloy CF8C plus at 800 C

    SciTech Connect

    Kumar, Deepak; Dryepondt, Sebastien N; Shyam, Amit; Haynes, James A; Pint, Bruce A; Armstrong, Beth L; Lara-Curzio, Edgar

    2011-01-01

    The cost effective, austenitic stainless steel CF8C plus is an attractive alloy for massive cast structures such as steam turbine casings. The microstructure stability and creep strength of this alloy are better than commercial high-performance heat-resistant steels such as NF709 and Super 304H, and are comparable to the Ni-based superalloy Inconel 617. The oxidation resistance of the alloy in atmosphere rich in water vapor is however insufficient at T>800 C, and the use of diffusion aluminide coatings is considered for potential high temperature applications. The thermal stability and protectiveness of coatings applied on the CF8C plus substrate by pack cementation and slurry process were investigated in air + 10% H2O environment at 800 C. Further, the coating effect on the fatigue life of the alloy was assessed via low-cycle-fatigue experiments.

  7. Lateral aerodynamic parameters extracted from flight data for the F-8C airplane in maneuvering flight

    NASA Technical Reports Server (NTRS)

    Suit, W. T.

    1977-01-01

    Flight test data are used to extract the lateral aerodynamic parameters of the F-8C airplane at moderate to high angles of attack. The data were obtained during perturbations of the airplane from steady turns with trim normal accelerations from 1.5g to 3.0g. The angle-of-attack variation from trim was negligible. The aerodynamic coefficients extracted from flight data were compared with several other sets of coefficients, and the extracted coefficients resulted in characteristics for the Dutch roll mode (at the highest angles of attack) similar to those of a set of coefficients that have been the basis of several simulations of the F-8C.

  8. Wibault two-seat monoplane 8C2 : an all-metal pursuit and observation airplane

    NASA Technical Reports Server (NTRS)

    Serryer, J

    1926-01-01

    Michel Wibault's two seat monoplane 8C2, is similar to the Parasol pursuit monoplane which preceded it. It has no perishable parts in its structure and needs no special storage or coverings. The sample aeroplane uses a 500 HP Hispano-Suiza engine but can accept a 400-600HP engine from a variety of manufacturers with little difficulty. It uses a two blade tractor propeller.

  9. Toward the Total Synthesis of Amphidinolide N: Synthesis of the C8-C29 Fragment.

    PubMed

    Kawashima, Yuki; Toyoshima, Atsushi; Fuwa, Haruhiko; Sasaki, Makoto

    2016-05-01

    A synthesis of the C8-C29 fragment of amphidinolide N, a potent cytotoxic macrolide isolated from the marine dinoflagellate Amphidinium sp., has been achieved. The key features of the synthesis involve a convergent union of the C9-C15 and C16-C29 fragments by Steglich esterification and the construction of a pyran unit through a Tebbe methylenation/ring-closing metathesis sequence. PMID:27116189

  10. Pressure distribution for the wing of the YAV-8B airplane; with and without pylons

    NASA Technical Reports Server (NTRS)

    Saltzman, Edwin J.; Delfrate, John H.; Sabsay, Catherine M.; Yarger, Jill M.

    1992-01-01

    Pressure distribution data have been obtained in flight at four span stations on the wing panel of the YAV-8B airplane. Data obtained for the supercritical profiled wing, with and without pylons installed, ranged from Mach 0.46 to 0.88. The altitude ranged from approximately 20,000 to 40,000 ft and the resultant Reynolds numbers varied from approximately 7.2 million to 28.7 million based on the mean aerodynamic chord. Pressure distribution data and flow visualization results show that the full-scale flight wing performance is compromised because the lower surface cusp region experiences flow separation for some important transonic flight conditions. This condition is aggravated when local shocks occur on the lower surface of the wing (mostly between 20 and 35 percent chord) when the pylons are installed for Mach 0.8 and above. There is evidence that convex fairings, which cover the pylon attachment flanges, cause these local shocks. Pressure coefficients significantly more negative than those for sonic flow also occur farther aft on the lower surface (near 60 percent chord) whether or not the pylons are installed for Mach numbers greater than or equal to 0.8. These negative pressure coefficient peaks and associated local shocks would be expected to cause increasing wave and separation drag at transonic Mach number increases.

  11. 7Be(p,(gamma))8B S-factor from Ab Initio No-Core Shell Model Wave Functions

    SciTech Connect

    Navratil, P; Bertulani, C A; Caurier, E

    2005-12-02

    Nuclear structure of {sup 7}Be, {sup 8}B and {sup 7,8}Li is studied within the ab initio no-core shell model (NCSM). Starting from high-precision nucleon-nucleon (NN) interactions, wave functions of {sup 7}Be and {sup 8}B bound states are obtained in basis spaces up to 10 h bar{Omega} and used to calculate channel cluster form factors (overlap integrals) of the {sup 8}B ground state with {sup 7}Be+p. Due to the use of the harmonic oscillator (HO) basis, the overlap integrals have incorrect asymptotic properties. We fix this problem in two alternative ways. First, by a Woods-Saxon (WS) potential solution fit to the interior of the NCSM overlap integrals. Second, by a direct matching with the Whittaker function. The corrected overlap integrals are then used for the {sup 7}Be(p,{gamma}){sup 8}B S-factor calculation. We study the convergence of the S-factor with respect to the NCSM HO frequency and the model space size. Our S-factor results are in agreement with recent direct measurement data. We also test the spectroscopic factors and the corrected overlap integrals from the NCSM in describing the momentum distributions in knockout reactions with {sup 8}B projectiles. A good agreement with the available experimental data is also found, attesting the overall consistency of the calculations.

  12. Evidence for a functional subdivision of Premotor Ear-Eye Field (Area 8B)

    PubMed Central

    Lanzilotto, Marco; Perciavalle, Vincenzo; Lucchetti, Cristina

    2015-01-01

    The Supplementary Eye Field (SEF) and the Frontal Eye Field (FEF) have been described as participating in gaze shift control. Recent evidence suggests, however, that other areas of the dorsomedial prefrontal cortex also influence gaze shift. Herein, we have investigated electrically evoked ear- and eye movements from the Premotor Ear-Eye Field, or PEEF (area 8B) of macaque monkeys. We stimulated PEEF during spontaneous condition (outside the task performance) and during the execution of a visual fixation task (VFT). In the first case, we functionally identified two regions within the PEEF: a core and a belt. In the core region, stimulation elicited forward ear movements; regarding the evoked eye movements, in some penetrations, stimulation elicited contraversive fixed-vectors with a mean amplitude of 5.14°; while in other penetrations, we observed prevalently contralateral goal-directed eye movements having end-points that fell within 15° in respect to the primary eye position. On the contrary, in the belt region, stimulation elicited backward ear movements; regarding the eye movements, in some penetrations stimulation elicited prevalently contralateral goal-directed eye movements having end-points that fell within 15° in respect to the primary eye position, while in the lateral edge of the investigated region, stimulation elicited contralateral goal-directed eye movements having end-points that fell beyond 15° in respect to the primary eye position. Stimulation during VFT either did not elicit eye movements or evoked saccades of only a few degrees. Finally, even though no head rotation movements were observed during the stimulation period, we viewed a relationship between the duration of stimulation and the neck forces exerted by the monkey's head. We propose an updated vision of the PEEF composed of two functional regions, core and belt, which may be involved in integrating auditory and visual information important to the programming of gaze orienting

  13. DISCOVERY AND ROSSITER-McLAUGHLIN EFFECT OF EXOPLANET KEPLER-8b

    SciTech Connect

    Jenkins, Jon M.; Caldwell, Douglas A.; Marcy, Geoffrey W.; Basri, Gibor; Isaacson, Howard; Cochran, William D.; Endl, Michael; Welsh, William F.; Batalha, Natalie M.; Buchhave, Lars A.; Geary, John C.; Brown, Timothy M.; Dunham, Edward W.; Fischer, Debra A.; Gautier, Thomas N.; Howell, Steve B.; Johnson, John Asher

    2010-12-01

    We report on the discovery and the Rossiter-McLaughlin (R-M) effect of Kepler-8b, a transiting planet identified by the NASA Kepler Mission. Kepler photometry and Keck-HIRES radial velocities yield the radius and mass of the planet around this F8IV subgiant host star. The planet has a radius R{sub P} = 1.419 R{sub J} and a mass M{sub P} = 0.60 M{sub J}, yielding a density of 0.26 g cm{sup -3}, one of the lowest planetary densities known. The orbital period is P = 3.523 days and the orbital semimajor axis is 0.0483{sup +0.0006}{sub -0.0012} AU. The star has a large rotational vsin i of 10.5 {+-} 0.7 km s{sup -1} and is relatively faint (V {approx} 13.89 mag); both properties are deleterious to precise Doppler measurements. The velocities are indeed noisy, with scatter of 30 m s{sup -1}, but exhibit a period and phase that are consistent with those implied by transit photometry. We securely detect the R-M effect, confirming the planet's existence and establishing its orbit as prograde. We measure an inclination between the projected planetary orbital axis and the projected stellar rotation axis of {lambda} = -26.{sup 0}4 {+-} 10.{sup 0}1, indicating a significant inclination of the planetary orbit. R-M measurements of a large sample of transiting planets from Kepler will provide a statistically robust measure of the true distribution of spin-orbit orientations for hot Jupiters around F and early G stars.

  14. Discovery and Rossiter-Mclaughlin Effect of Exoplanet Kepler-8b

    NASA Astrophysics Data System (ADS)

    Jenkins, Jon M.; Borucki, William J.; Koch, David G.; Marcy, Geoffrey W.; Cochran, William D.; Welsh, William F.; Basri, Gibor; Batalha, Natalie M.; Buchhave, Lars A.; Brown, Timothy M.; Caldwell, Douglas A.; Dunham, Edward W.; Endl, Michael; Fischer, Debra A.; Gautier, Thomas N., III; Geary, John C.; Gilliland, Ronald L.; Howell, Steve B.; Isaacson, Howard; Johnson, John Asher; Latham, David W.; Lissauer, Jack J.; Monet, David G.; Rowe, Jason F.; Sasselov, Dimitar D.; Howard, Andrew W.; MacQueen, Phillip; Orosz, Jerome A.; Chandrasekaran, Hema; Twicken, Joseph D.; Bryson, Stephen T.; Quintana, Elisa V.; Clarke, Bruce D.; Li, Jie; Allen, Christopher; Tenenbaum, Peter; Wu, Hayley; Meibom, Søren; Klaus, Todd C.; Middour, Christopher K.; Cote, Miles T.; McCauliff, Sean; Girouard, Forrest R.; Gunter, Jay P.; Wohler, Bill; Hall, Jennifer R.; Ibrahim, Khadeejah; Kamal Uddin, AKM; Wu, Michael S.; Bhavsar, Paresh A.; Van Cleve, Jeffrey; Pletcher, David L.; Dotson, Jessie L.; Haas, Michael R.

    2010-12-01

    We report on the discovery and the Rossiter-McLaughlin (R-M) effect of Kepler-8b, a transiting planet identified by the NASA Kepler Mission. Kepler photometry and Keck-HIRES radial velocities yield the radius and mass of the planet around this F8IV subgiant host star. The planet has a radius R P = 1.419 R J and a mass M P = 0.60 M J, yielding a density of 0.26 g cm-3, one of the lowest planetary densities known. The orbital period is P = 3.523 days and the orbital semimajor axis is 0.0483+0.0006 -0.0012 AU. The star has a large rotational vsin i of 10.5 ± 0.7 km s-1 and is relatively faint (V ≈ 13.89 mag); both properties are deleterious to precise Doppler measurements. The velocities are indeed noisy, with scatter of 30 m s-1, but exhibit a period and phase that are consistent with those implied by transit photometry. We securely detect the R-M effect, confirming the planet's existence and establishing its orbit as prograde. We measure an inclination between the projected planetary orbital axis and the projected stellar rotation axis of λ = -26fdg4 ± 10fdg1, indicating a significant inclination of the planetary orbit. R-M measurements of a large sample of transiting planets from Kepler will provide a statistically robust measure of the true distribution of spin-orbit orientations for hot Jupiters around F and early G stars. Based in part on observations obtained at the W. M. Keck Observatory, which is operated as a scientific partnership between the California Institute of Technology, the University of California, and the National Aeronautics and Space Administration. The Observatory was made possible by the generous financial support of the W. M. Keck Foundation.

  15. Parametric Time-Dependent Navier-Stokes Computations for a YAV-8B Harrier in Ground Effect

    NASA Technical Reports Server (NTRS)

    Chaderjian, Neal M.; Pandya, Shishir; Ahmad, Jasim; Murman, Scott; Kwak, Dochan (Technical Monitor)

    2002-01-01

    The Harrier Jump Jet has the distinction of being the only powered-lift aircraft in the free world to achieve operational status and to have flown in combat. This V/STOL aircraft can take-off and land vertically or utilize very short runways by directing its four exhaust nozzles towards the ground. Transition to forward flight is achieved by rotating these nozzles into a horizontal position. Powered-lift vehicles have certain advantages over conventional strike fighters. Their V/STOL capabilities allow for safer carrier operations, smaller carrier size, and quick reaction time for troop support. Moreover, they are not dependent on vulnerable land-based runways. The AV-8A Harrier first entered service in the British Royal Air Force (RAF) during 1969, and the U.S. Marine Corps (USMC) in 1971. The AV-8B was a redesign to achieve improved payload capacity, range, and accuracy. This modified design first entered service with the USMC and RAF in 1985. The success and unique capabilities of the Harrier has prompted the design of a powered-lift version of the Joint Strike Fighter (JSF). The flowfield for the Harrier near the ground during low-speed or hover flight operations is very complex and time-dependent. A sketch of this flowfield is shown. Warm air from the fan is exhausted from the front nozzles, while a hot air/fuel mixture from the engine is exhausted from the rear nozzles. These jets strike the ground and move out radially forming a ground jet-flow. The ambient freestream, due to low-speed forward flight or - headwind during hover, opposes the jet-flow. This interaction causes the flow to separate and form a ground vortex. The multiple jets also interact with each other near the ground and form an upwash or jet fountain, which strikes the underside of the fuselage. If the aircraft is sufficiently close to the ground, the inlet can ingest ground debris and hot gases from the fountain and ground vortex. This Hot Gas Ingestion (HGI) can cause a sudden loss of

  16. Sc2@C3v(8)-C82 vs. Sc2C2@C3v(8)-C82: drastic effect of C2 capture on the redox properties of scandium metallofullerenes.

    PubMed

    Kurihara, Hiroki; Lu, Xing; Iiduka, Yuko; Mizorogi, Naomi; Slanina, Zdenek; Tsuchiya, Takahiro; Nagase, Shigeru; Akasaka, Takeshi

    2012-01-30

    We describe the first example of scandium dimetallofullerenes, Sc(2)@C(3v)(8)-C(82), which has the same cage as the previously assigned scandium carbide cluster fullerene Sc(2)C(2)@C(3v)(8)-C(82) but they exhibit distinctly different electronic configurations and electronic behaviours, confirming the drastic influence of the internal C(2) unit.

  17. 17 CFR 239.16 - Form S-6, for unit investment trusts registered on Form N-8B-2.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Form S-6, for unit investment... § 239.16 Form S-6, for unit investment trusts registered on Form N-8B-2. This form may be used for registration under the Securities Act of 1933 of securities of any unit investment trust registered under...

  18. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... under the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8B-4, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... SECURITIES AND EXCHANGE COMMISSION (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF...

  19. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... under the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8B-4, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... SECURITIES AND EXCHANGE COMMISSION (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF...

  20. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... under the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8B-4, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... SECURITIES AND EXCHANGE COMMISSION (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF...

  1. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... under the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8B-4, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... SECURITIES AND EXCHANGE COMMISSION (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF...

  2. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... under the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8B-4, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... SECURITIES AND EXCHANGE COMMISSION (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF...

  3. SEARCH FOR GLOBAL f-MODES AND p-MODES IN THE {sup 8}B NEUTRINO FLUX

    SciTech Connect

    Lopes, Ilídio E-mail: ilopes@uevora.pt

    2013-11-01

    The impact of global acoustic modes on the {sup 8}B neutrino flux time series is computed for the first time. It is shown that the time fluctuations of the {sup 8}B neutrino flux depend on the amplitude of acoustic eigenfunctions in the region where the {sup 8}B neutrino flux is produced: modes with low n (or order) that have eigenfunctions with a relatively large amplitude in the Sun's core strongly affect the neutrino flux; conversely, modes with high n that have eigenfunctions with a minimal amplitude in the Sun's core have a very small impact on the neutrino flux. It was found that the global modes with a larger impact on the {sup 8}B neutrino flux have a frequency of oscillation in the interval 250 μHz to 500 μHz (or a period in the interval 30 minutes to 70 minutes), such as the f-modes (n = 0) for the low degrees, radial modes of order n ≤ 3, and the dipole mode of order n = 1. Their corresponding neutrino eigenfunctions are very sensitive to the solar inner core and are unaffected by the variability of the external layers of the solar surface. If time variability of neutrinos is observed for these modes, it will lead to new ways of improving the sound speed profile inversion in the central region of the Sun.

  4. A Functional Link between AMPK and Orexin Mediates the Effect of BMP8B on Energy Balance.

    PubMed

    Martins, Luís; Seoane-Collazo, Patricia; Contreras, Cristina; González-García, Ismael; Martínez-Sánchez, Noelia; González, Francisco; Zalvide, Juan; Gallego, Rosalía; Diéguez, Carlos; Nogueiras, Rubén; Tena-Sempere, Manuel; López, Miguel

    2016-08-23

    AMP-activated protein kinase (AMPK) in the ventromedial nucleus of the hypothalamus (VMH) and orexin (OX) in the lateral hypothalamic area (LHA) modulate brown adipose tissue (BAT) thermogenesis. However, whether these two molecular mechanisms act jointly or independently is unclear. Here, we show that the thermogenic effect of bone morphogenetic protein 8B (BMP8B) is mediated by the inhibition of AMPK in the VMH and the subsequent increase in OX signaling via the OX receptor 1 (OX1R). Accordingly, the thermogenic effect of BMP8B is totally absent in ox-null mice. BMP8B also induces browning of white adipose tissue (WAT), its thermogenic effect is sexually dimorphic (only observed in females), and its impact on OX expression and thermogenesis is abolished by the knockdown of glutamate vesicular transporter 2 (VGLUT2), implicating glutamatergic signaling. Overall, our data uncover a central network controlling energy homeostasis that may be of considerable relevance for obesity and metabolic disorders. PMID:27524625

  5. Hydrocarbons in the C8--C20 range measured during COAST study in Texas

    SciTech Connect

    Zielinska, B.; Sagebiel, J.; Sheetz, L.H.; Harshfield, G.; Uberna, E.; Hauze, W.J.; Price, J.H.

    1994-12-31

    Hydrocarbons in the C8--C20 range were collected using Tenax-TA solid adsorbent cartridges during the Coastal Oxidant Assessment for Southeast Texas (COAST) field study, carried out in the Houston-Galveston and Beaumont-Port Arthur areas. Ambient samples were collected at the Galleria, Clinton and Port Arthur sampling sites, from August 26 to August 30, 1993. In addition, a number of Tenax samples were collected in the vicinity of major industrial complexes (such as Exxon, AMOCO Oil, Union Carbide, Dow Chemical) and in an urban tunnel. All samples were analyzed with high resolution capillary column gas chromatography with flame ionization detection (GC/FID) for quantification and selected duplicate samples were analyzed with combined gas chromatography/Fourier transform infrared/mass spectrometry (GC/FTIR/MS) for identification of individual compounds. The results of these analyses are presented.

  6. Inclusion body hepatitis associated with an outbreak of fowl adenovirus type 2 and type 8b in broiler flocks in South Africa.

    PubMed

    Maartens, Louis H; Joubert, Hilda W; Aitchison, Henry; Venter, Estelle H

    2015-01-01

    Inclusion body hepatitis is an acute disease of chickens ascribed to viruses of the genus Aviadenovirus and referred to as fowl adenovirus (FAdV). There are 12 FAdV types (FAdV1to FAdV8a and FAdV8b to FAdV11), classified into five species based on their genotype (designated FAdVA to FAdVE). A total of 218 000 chickens, 2-29 days of age, were affected over a 1-year period, all testing positive by microscopy, virus isolation and confirmation with polymerase chain reaction (PCR). Affected birds were depressed, lost body weight,were weak and had watery droppings. Pathological changes observed during necropsy indicated consistent changes in the liver, characterised by hepatomegaly, cholestasis and hepatitis. Lesions were also discernible in the spleen, kidney and gizzard wall and were characterised by splenomegaly, pinpoint haemorrhages, nephritis with haemorrhage,visceral gout and serosal ecchymosis of the gizzard wall. Histopathological lesions were most consistently observed in the liver but could also be seen in renal and splenic tissue. Virus isolation was achieved in embryonated eggs and most embryos revealed multifocalto diffuse hepatic necrosis, with a mixed cellular infiltrate of macrophages and heterophils(necro-granulomas), even in the absence of macroscopic pathology. Virus isolation results were verified by histopathology and PCR on embryonic material and further characterised by nucleotide sequence analysis. Two infectious bursal disease virus isolates were also made from the Klerksdorp flock. Nucleotide sequence analysis of the L1 hexon loop of all the FAdV isolates indicated homology (99%) with prototype strains P7-A for FAdV-2, as well as for FAdV-8b.

  7. Evidence for electron neutrino flavor change through measurement of the (8)B solar neutrino flux at the Sudbury Neutrino Observatory

    NASA Astrophysics Data System (ADS)

    Neubauer, Mark Stephen

    2001-11-01

    The Sudbury Neutrino Observatory (SNO) is a water Cerenkov detector designed to study solar neutrinos. Using 1 kiloton of heavy water as the target and detection medium, SNO is able to separately determine the flux of electron neutrinos (νe) and the flux of all active neutrinos from the Sun by measuring the rate of charged current (CC) and neutral current (NC) interactions with deuterons. A comparison of these interaction rates allows for direct observation of solar neutrino oscillations. SNO can also search for oscillations by comparing the rate of CC and neutrino- electron elastic scattering (ES) events, since ES has both charged current and neutral current sensitivity. In this thesis, we present measurement of the 8B solar ν e flux of 1.78+0.13-0.14 (stat+syst) × 106cm-2s -1 (35% BP2000 SSM) through measurement of the CC rate over 169.3 days of livetime. We have also measured the 8B flux from the ES reaction to be 2.56+0.48-0.45 (stat+syst), consistent with measurements by previous water Cerenkov experiments. A flavor analysis comparing the CC measured flux with that determined through ES by SuperKamiokande yields a non- νe active neutrino flux from 8B of 3.62+1.06-1.08 × 106cm-2s-1 , providing evidence for νe --> ν μ,τ oscillations as a solution to the solar neutrino problem. This result excludes pure solar νe --> ν s oscillations at greater than the 99.7% C.I. The total active 8B neutrino flux has been measured to be 5.39+1.07-1.09 × 106cm-2s-1 , consistent with BP2000 SSM predictions. First analyses of the CC (NHit) spectrum and hep flux in SNO are presented. The CC spectrum is found to be a good fit to expectations from an undistorted 8B spectrum, and global best fit vacuum oscillation solutions are disfavored over the other solutions by the data. Through observations near the 8B endpoint with consideration of energy systematics, hep flux limits of 4.1 (90% C.I.) and 6.9 (99% C.I.) times SSM expectations are obtained. A statistical fit for the

  8. Measurement of the {sup 8}B solar neutrino flux with the KamLAND liquid scintillator detector

    SciTech Connect

    Abe, S.; Furuno, K.; Gando, A.; Gando, Y.; Ichimura, K.; Ikeda, H.; Kibe, Y.; Kimura, W.; Kishimoto, Y.; Minekawa, Y.; Mitsui, T.; Morikawa, T.; Nagai, N.; Nakajima, K.; Nakamura, M.; Narita, K.; Shimizu, I.; Shimizu, Y.; Shirai, J.; Suekane, F.

    2011-09-15

    We report a measurement of the neutrino-electron elastic scattering rate from {sup 8}B solar neutrinos based on a 123 kton-day exposure of KamLAND. The background-subtracted electron recoil rate, above a 5.5-MeV analysis threshold is 1.49 {+-} 0.14(stat) {+-} 0.17(syst) events per kton-day. Interpreted as due to a pure electron flavor flux with a {sup 8}B neutrino spectrum, this corresponds to a spectrum integrated flux of 2.77 {+-} 0.26(stat) {+-} 0.32(syst) x10{sup 6} cm{sup -2}s{sup -1}. The analysis threshold is driven by {sup 208}Tl present in the liquid scintillator, and the main source of systematic uncertainty is due to background from cosmogenic {sup 11}Be. The measured rate is consistent with existing measurements and with standard solar model predictions which include matter-enhanced neutrino oscillation.

  9. Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea

    SciTech Connect

    Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; Sà, Elisabet Laia; Ignacio-Espinoza, J. Cesar; Cornejo-Castillo, Francisco M.; Verberkmoes, Nathan C.; Vaqué, Dolors; Sullivan, Matthew B.; Acinas, Silvia G.; Kellogg, Christina A.

    2015-01-14

    Marine viruses (phages) alter bacterial diversity and evolution with impacts on marine biogeochemical cycles, and yet few well-developed model systems limit opportunities for hypothesis testing. We isolate phage B8b from the Mediterranean Sea using Pseudoalteromonas sp. QC-44 as a host and characterize it using myriad techniques. Morphologically, phage B8b was classified as a member of the Siphoviridae family. One-step growth analyses showed that this siphovirus had a latent period of 70 min and released 172 new viral particles per cell. In the host range analysis against 89 bacterial host strains revealed that phage B8b infected 3 Pseudoalteromonas strains (52 tested, >99.9% 16S rRNA gene nucleotide identity) and 1 non-Pseudoaltermonas strain belonging to Alteromonas sp. (37 strains from 6 genera tested), which helps bound the phylogenetic distance possible in a phage-mediated horizontal gene transfer event. The Pseudoalteromonas phage B8b genome size was 42.7 kb, with clear structural and replication modules where the former were delineated leveraging identification of 16 structural genes by virion structural proteomics, only 4 of which had any similarity to known structural proteins. In nature, this phage was common in coastal marine environments in both photic and aphotic layers (found in 26.5% of available viral metagenomes), but not abundant in any sample (average per sample abundance was 0.65% of the reads). Together these data improve our understanding of siphoviruses in nature, and provide foundational information for a new 'rare virosphere' phage-host model system.

  10. Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea

    DOE PAGES

    Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; Sà, Elisabet Laia; Ignacio-Espinoza, J. Cesar; Cornejo-Castillo, Francisco M.; Verberkmoes, Nathan C.; Vaqué, Dolors; Sullivan, Matthew B.; Acinas, Silvia G.; et al

    2015-01-14

    Marine viruses (phages) alter bacterial diversity and evolution with impacts on marine biogeochemical cycles, and yet few well-developed model systems limit opportunities for hypothesis testing. We isolate phage B8b from the Mediterranean Sea using Pseudoalteromonas sp. QC-44 as a host and characterize it using myriad techniques. Morphologically, phage B8b was classified as a member of the Siphoviridae family. One-step growth analyses showed that this siphovirus had a latent period of 70 min and released 172 new viral particles per cell. In the host range analysis against 89 bacterial host strains revealed that phage B8b infected 3 Pseudoalteromonas strains (52 tested,more » >99.9% 16S rRNA gene nucleotide identity) and 1 non-Pseudoaltermonas strain belonging to Alteromonas sp. (37 strains from 6 genera tested), which helps bound the phylogenetic distance possible in a phage-mediated horizontal gene transfer event. The Pseudoalteromonas phage B8b genome size was 42.7 kb, with clear structural and replication modules where the former were delineated leveraging identification of 16 structural genes by virion structural proteomics, only 4 of which had any similarity to known structural proteins. In nature, this phage was common in coastal marine environments in both photic and aphotic layers (found in 26.5% of available viral metagenomes), but not abundant in any sample (average per sample abundance was 0.65% of the reads). Together these data improve our understanding of siphoviruses in nature, and provide foundational information for a new 'rare virosphere' phage-host model system.« less

  11. Life-style and genome structure of marine Pseudoalteromonas siphovirus B8b isolated from the northwestern Mediterranean Sea.

    PubMed

    Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; Sà, Elisabet Laia; Ignacio-Espinoza, J Cesar; Cornejo-Castillo, Francisco M; Verberkmoes, Nathan C; Vaqué, Dolors; Sullivan, Matthew B; Acinas, Silvia G

    2015-01-01

    Marine viruses (phages) alter bacterial diversity and evolution with impacts on marine biogeochemical cycles, and yet few well-developed model systems limit opportunities for hypothesis testing. Here we isolate phage B8b from the Mediterranean Sea using Pseudoalteromonas sp. QC-44 as a host and characterize it using myriad techniques. Morphologically, phage B8b was classified as a member of the Siphoviridae family. One-step growth analyses showed that this siphovirus had a latent period of 70 min and released 172 new viral particles per cell. Host range analysis against 89 bacterial host strains revealed that phage B8b infected 3 Pseudoalteromonas strains (52 tested, >99.9% 16S rRNA gene nucleotide identity) and 1 non-Pseudoaltermonas strain belonging to Alteromonas sp. (37 strains from 6 genera tested), which helps bound the phylogenetic distance possible in a phage-mediated horizontal gene transfer event. The Pseudoalteromonas phage B8b genome size was 42.7 kb, with clear structural and replication modules where the former were delineated leveraging identification of 16 structural genes by virion structural proteomics, only 4 of which had any similarity to known structural proteins. In nature, this phage was common in coastal marine environments in both photic and aphotic layers (found in 26.5% of available viral metagenomes), but not abundant in any sample (average per sample abundance was 0.65% of the reads). Together these data improve our understanding of siphoviruses in nature, and provide foundational information for a new 'rare virosphere' phage-host model system. PMID:25587991

  12. Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea

    PubMed Central

    Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; Sà, Elisabet Laia; Ignacio-Espinoza, J. Cesar; Cornejo-Castillo, Francisco M.; Verberkmoes, Nathan C.; Vaqué, Dolors; Sullivan, Matthew B.; Acinas, Silvia G.

    2015-01-01

    Marine viruses (phages) alter bacterial diversity and evolution with impacts on marine biogeochemical cycles, and yet few well-developed model systems limit opportunities for hypothesis testing. Here we isolate phage B8b from the Mediterranean Sea using Pseudoalteromonas sp. QC-44 as a host and characterize it using myriad techniques. Morphologically, phage B8b was classified as a member of the Siphoviridae family. One-step growth analyses showed that this siphovirus had a latent period of 70 min and released 172 new viral particles per cell. Host range analysis against 89 bacterial host strains revealed that phage B8b infected 3 Pseudoalteromonas strains (52 tested, >99.9% 16S rRNA gene nucleotide identity) and 1 non-Pseudoaltermonas strain belonging to Alteromonas sp. (37 strains from 6 genera tested), which helps bound the phylogenetic distance possible in a phage-mediated horizontal gene transfer event. The Pseudoalteromonas phage B8b genome size was 42.7 kb, with clear structural and replication modules where the former were delineated leveraging identification of 16 structural genes by virion structural proteomics, only 4 of which had any similarity to known structural proteins. In nature, this phage was common in coastal marine environments in both photic and aphotic layers (found in 26.5% of available viral metagenomes), but not abundant in any sample (average per sample abundance was 0.65% of the reads). Together these data improve our understanding of siphoviruses in nature, and provide foundational information for a new ‘rare virosphere’ phage–host model system. PMID:25587991

  13. Range 8C Rehabilitation Demonstration Project, Hohenfels Training Area, Germany: Final report

    SciTech Connect

    Zellmer, S.D.; Hinchman, R.R.; Johnson, D.O.; Severinghaus, W.D.; Brent, J.J.

    1991-11-01

    More than 30 years of intensive and continual tactical training has caused extensive environmental damage at the US Army Hohenfels Training Area in Germany. The Range 8C Rehabilitation Demonstration Project, followed by a three-year monitoring effort, was conducted to develop and evaluate the environmental and economic effectiveness of seven revegetation and four erosion control prescriptions implemented at a 16-ha site. The point-intercept method was used to measure the types and amounts of vegetation established and the changes in the vegetative community during three years of military use on the seven areas treated with revegetation prescriptions. Field observations were made to determine the suitability and durability of four types of erosion control structures. Soil fertility and a source of seed appeared to be the most limiting factors in establishing vegetation, while seedbed preparation had only a minor influence. Grasses appeared to be more resistant to vehicle traffic than did other types of vegetation. Because grassed waterways were used as roads by military vehicles and a system of graded terraces was expensive, these erosion control prescriptions were unsuitable and uneconomical for use on training areas. Low-cost riprap waterbars and porous check dams slowed the velocity of runoff, trapped sediments, and were durable. Recommendations were formulated to improve the environmental and economic effectiveness of future rehabilitation efforts on tactical training areas.

  14. Improving a recombinant Zymomonas mobilis strain 8b through continuous adaptation on dilute acid pretreated corn stover hydrolysate

    DOE PAGES

    Mohagheghi, Ali; Linger, Jeffrey G.; Yang, Shihui; Smith, Holly; Dowe, Nancy; Zhang, Min; Pienkos, Philip T.

    2015-03-31

    Complete conversion of the major sugars of biomass including both the C5 and C6 sugars is critical for biofuel production processes. Several inhibitory compounds like acetate, hydroxymethylfurfural (HMF), and furfural are produced from the biomass pretreatment process leading to ‘hydrolysate toxicity,’ a major problem for microorganisms to achieve complete sugar utilization. Therefore, development of more robust microorganisms to utilize the sugars released from biomass under toxic environment is critical. In this study, we use continuous culture methodologies to evolve and adapt the ethanologenic bacterium Zymomonas mobilis to improve its ethanol productivity using corn stover hydrolysate. The results are the following:more » A turbidostat was used to adapt the Z. mobilis strain 8b in the pretreated corn stover liquor. The adaptation was initiated using pure sugar (glucose and xylose) followed by feeding neutralized liquor at different dilution rates. Once the turbidostat reached 60% liquor content, the cells began washing out and the adaptation was stopped. Several ‘sub-strains’ were isolated, and one of them, SS3 (sub-strain 3), had 59% higher xylose utilization than the parent strain 8b when evaluated on 55% neutralized PCS (pretreated corn stover) liquor. Using saccharified PCS slurry generated by enzymatic hydrolysis from 25% solids loading, SS3 generated an ethanol yield of 75.5% compared to 64% for parent strain 8b. Furthermore, the total xylose utilization was 57.7% for SS3 versus 27.4% for strain 8b. To determine the underlying genotypes in these new sub-strains, we conducted genomic resequencing and identified numerous single-nucleotide mutations (SNPs) that had arisen in SS3. We further performed quantitative reverse transcription PCR (qRT-PCR) on genes potentially affected by these SNPs and identified significant down-regulation of two genes, ZMO0153 and ZMO0776, in SS3 suggesting potential genetic mechanisms behind SS3’s improved

  15. [Clinical feature and ATP8B1 mutation analysis of a patient with progressive familial intrahepatic cholestasis type I].

    PubMed

    Cheng, Ying; Guo, Li; Song, Yuan-Zong

    2016-08-01

    Progressive familial intrahepatic cholestasis type I (PFIC1) is an autosomal recessive disorder caused by biallelic mutations of ATP8B1 gene, with progressive cholestasis as the main clinical manifestation. This paper reports the clinical and genetic features of a PFIC1 patient definitely diagnosed by ATP8B1 genetic analysis. The patient, a boy aged 14 months, was referred to the hospital with the complaint of jaundiced skin and sclera over 10 months. The patient had been managed in different hospitals, but the therapeutic effects were unsatisfactory due to undetermined etiology. On physical examination, hepatosplenomegaly was discovered in addition to jaundice of the skin and sclera. The liver was palpable 4 cm below the right subcostal margin and 2 cm below the xiphoid while the spleen 2 cm below the left subcostal margin. The liver function test revealed elevated levels of serum total bile acids, bilirubin, and transaminases; however, the γ-glutamyl transferase level was normal. The diagnosis was genetic cholestasis of undetermined origin. At the age of 1 year and 8 months, a Roux-en-Y cholecystocolonic bypass operation was performed, and thereafter the jaundice disappeared. At 5 years and 1 month, via whole genome sequencing analysis and Sanger sequencing confirmation, the boy was found to be a homozygote of mutation c.2081T>A(p.I694N) of ATP8B1 gene, and thus PFIC1 was definitely diagnosed. The boy was followed up until he was 6 years, and jaundice did not recur, but the long-term outcome remains to be observed. PMID:27530795

  16. EARLINET dust observations vs. BSC-DREAM8b modeled profiles: 12-year-long systematic comparison at Potenza, Italy

    NASA Astrophysics Data System (ADS)

    Mona, L.; Papagiannopoulos, N.; Basart, S.; Baldasano, J.; Binietoglou, I.; Cornacchia, C.; Pappalardo, G.

    2014-08-01

    In this paper, we report the first systematic comparison of 12-year modeled dust extinction profiles vs. Raman lidar measurements. We use the BSC-DREAM8b model, one of the most widely used dust regional models in the Mediterranean, and Potenza EARLINET lidar profiles for Saharan dust cases, the largest one-site database of dust extinction profiles. A total of 310 dust cases were compared for the May 2000-July 2012 period. The model reconstructs the measured layers well: profiles are correlated within 5% of significance for 60% of the cases and the dust layer center of mass as measured by lidar and modeled by BSC-DREAM8b differ on average 0.3 ± 1.0 km. Events with a dust optical depth lower than 0.1 account for 70% of uncorrelated profiles. Although there is good agreement in terms of profile shape and the order of magnitude of extinction values, the model overestimates the occurrence of dust layer top above 10 km. Comparison with extinction profiles measured by the Raman lidar shows that BSC-DREAM8b typically underestimates the dust extinction coefficient, in particular below 3 km. Lowest model-observation differences (below 17%) correspond to a lidar ratio at 532 nm and Ångström exponent at 355/532 nm of 60 ± 13 and 0.1 ± 0.6 sr, respectively. These are in agreement with values typically observed and modeled for pure desert dust. However, the highest differences (higher than 85%) are typically related to greater Ångström values (0.5 ± 0.6), denoting smaller particles. All these aspects indicate that the level of agreement decreases with an increase in mixing/modification processes.

  17. Pigs fed camelina meal increase hepatic gene expression of cytochrome 8b1, aldehyde dehydrogenase, and thiosulfate transferase

    PubMed Central

    2014-01-01

    Camelina sativa is an oil seed crop which can be grown on marginal lands. Camelina seed oil is rich in omega-3 fatty acids (>35%) and γ-tocopherol but is also high in erucic acid and glucosinolates. Camelina meal, is the by-product after the oil has been extracted. Camelina meal was fed to 28 d old weaned pigs at 3.7% and 7.4% until age 56 d. The camelina meal supplements in the soy based diets, improved feed efficiency but also significantly increased the liver weights. Gene expression analyses of the livers, using intra-species microarrays, identified increased expression of phase 1 and phase 2 drug metabolism enzymes. The porcine versions of the enzymes were confirmed by real time PCR. Cytochrome 8b1 (CYP8B1), aldehyde dehydrogenase 2 (Aldh2), and thiosulfate transferase (TST) were all significantly stimulated. Collectively, these genes implicate the camelina glucosinolate metabolite, methyl-sulfinyldecyl isothiocyanate, as the main xeniobiotic, causing increased hepatic metabolism and increased liver weight. PMID:24383433

  18. Heterologous expression of ATG8c from soybean confers tolerance to nitrogen deficiency and increases yield in Arabidopsis.

    PubMed

    Xia, Tongmei; Xiao, Dong; Liu, Dong; Chai, Wenting; Gong, Qingqiu; Wang, Ning Ning

    2012-01-01

    Nitrogen is an essential element for plant growth and yield. Improving Nitrogen Use Efficiency (NUE) of crops could potentially reduce the application of chemical fertilizer and alleviate environmental damage. To identify new NUE genes is therefore an important task in molecular breeding. Macroautophagy (autophagy) is an intracellular process in which damaged or obsolete cytoplasmic components are encapsulated in double membraned vesicles termed autophagosomes, then delivered to the vacuole for degradation and nutrient recycling. One of the core components of autophagosome formation, ATG8, has been shown to directly mediate autophagosome expansion, and the transcript of which is highly inducible upon starvation. Therefore, we postulated that certain homologs of Saccharomyces cerevisiae ATG8 (ScATG8) from crop species could have potential for NUE crop breeding. A soybean (Glycine max, cv. Zhonghuang-13) ATG8, GmATG8c, was selected from the 11 family members based on transcript analysis upon nitrogen deprivation. GmATG8c could partially complement the yeast atg8 mutant. Constitutive expression of GmATG8c in soybean callus cells not only enhanced nitrogen starvation tolerance of the cells but accelerated the growth of the calli. Transgenic Arabidopsis over-expressing GmATG8c performed better under extended nitrogen and carbon starvation conditions. Meanwhile, under optimum growth conditions, the transgenic plants grew faster, bolted earlier, produced larger primary and axillary inflorescences, eventually produced more seeds than the wild-type. In average, the yield was improved by 12.9%. We conclude that GmATG8c may serve as an excellent candidate for breeding crops with enhanced NUE and better yield. PMID:22629371

  19. A Validated HPLC Method for Simultaneous Determination of Caffeoyl Phenylethanoid Glucosides and Flavone 8-C-glycosides in Haberlea rhodopensis.

    PubMed

    Zheleva-Dimitrova, Dimitrina; Nedialkov, Paraskev; Giresser, Ulrich

    2016-06-01

    A HPLC-UV method for analysis of the main compounds: caffeoyl phenylethanoid glucosides myconoside (1) and paucifloside (2) and flavone 8-C-glycosides: hispidulin 8-C-β-galactopyranoside (3), hispidulin 8-C-(2"-O-syringoyl-β-glucopyranoside) (4), hispidulin 8-C-(6-O-acetyl-β-glucopyranoside) (5) and hispidulin 8-C-(6-O-acetyl-2"-O-syringoyl--glucopyranoside) (6) in Haberlea rhodopensis leaves was developed and validated. Compound 3 was isolated for the first time from the title species. Ultrasound extraction with 80% methanol at room temperature allowed a good recovery of analytes (from 87.2 % for 1 to 109.8 % for 3) and the precision of the entire procedure was between 1.6% and 6.9%. The subsequent HPLC separation and quantification was achieved using a Hypersil ODS C18 column and UV detection at 280 nm. The mobile phase comprised methanol and 0.1 % o-phosphoric acid, and gradient elution mode was applied. The detection limits ranged from 0.042 μg/mL (6) to 0.18 μg/mL (5). The total amount in leaves of the assayed phenolic compounds was 374.2 mg/g. Myconoside was found to be the dominant compound in H. rhodopensis extract (332.2 ± 0.7 mg/g dw) and reached up to 88.8% of the analyzed mixture in leaves, while the total content of flavone C-glycosides was 17.1 mg/g dw. PMID:27534117

  20. Screening a phage display library for a novel FGF8b-binding peptide with anti-tumor effect on prostate cancer

    SciTech Connect

    Wang, Wenhui; Chen, Xilei; Li, Tao; Li, Yanmei; Wang, Ruixue; He, Dan; Luo, Wu; Li, Xiaokun; Wu, Xiaoping

    2013-05-01

    Fibroblast growth factor 8b (FGF8b) is the major isoform of FGF8 expressed in prostate cancer and it correlates with the stage and grade of the disease. FGF8b has been considered as a potential target for prostate cancer therapy. Here we isolated 12 specific FGF8b-binding phage clones by screening a phage display heptapeptide library with FGF8b. The peptide (HSQAAVP, named as P12) corresponding to one of these clones showed high homology to the immunoglobulin-like (Ig-like) domain II(D2) of high-affinity FGF8b receptor (FGFR3c), contained 3 identical amino acids (AVP) to the authentic FGFR3 D2 sequence aa 163–169 (LLAVPAA) directly participating in ligand binding, carried the same charges as its corresponding motif (aa163–169) in FGFR3c, suggesting that P12 may have a greater potential to interrupt FGF8b binding to its receptors than other identified heptapeptides do. Functional analysis indicated that synthetic P12 peptides mediate significant inhibition of FGF8b-induced cell proliferation, arrest cell cycle at the G0/G1 phase via suppression of Cyclin D1 and PCNA, and blockade of the activations of Erk1/2 and Akt cascades in both prostate cancer cells and vascular endothelial cells. The results demonstrated that the P12 peptide acting as an FGF8b antagonist may have therapeutic potential in prostate cancer. - Highlights: ► A novel FGF8b-binding peptide P12 was isolated from a phage display library. ► The mechanisms for P12 peptide inhibiting cell proliferation were proposed. ► P12 caused cell cycle arrest at G0/G1 phase via suppression of Cyclin D1 and PCNA. ► P12 suppressed FGF8b-induced activations of Akt and MAP kinases. ► P12 acting as an FGF8b antagonist may have therapeutic potential in prostate cancer.

  1. Measurement of the total active 8B solar neutrino flux at the Sudbury Neutrino Observatory with enhanced neutral current sensitivity.

    PubMed

    Ahmed, S N; Anthony, A E; Beier, E W; Bellerive, A; Biller, S D; Boger, J; Boulay, M G; Bowler, M G; Bowles, T J; Brice, S J; Bullard, T V; Chan, Y D; Chen, M; Chen, X; Cleveland, B T; Cox, G A; Dai, X; Dalnoki-Veress, F; Doe, P J; Dosanjh, R S; Doucas, G; Dragowsky, M R; Duba, C A; Duncan, F A; Dunford, M; Dunmore, J A; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Formaggio, J A; Fowler, M M; Frame, K; Fulsom, B G; Gagnon, N; Graham, K; Grant, D R; Hahn, R L; Hall, J C; Hallin, A L; Hallman, E D; Hamer, A S; Handler, W B; Hargrove, C K; Harvey, P J; Hazama, R; Heeger, K M; Heintzelman, W J; Heise, J; Helmer, R L; Hemingway, R J; Hime, A; Howe, M A; Jagam, P; Jelley, N A; Klein, J R; Kos, M S; Krumins, A V; Kutter, T; Kyba, C C M; Labranche, H; Lange, R; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Levine, I; Luoma, S; MacLellan, R; Majerus, S; Mak, H B; Maneira, J; Marino, A D; McCauley, N; McDonald, A B; McGee, S; McGregor, G; Mifflin, C; Miknaitis, K K S; Miller, G G; Moffat, B A; Nally, C W; Nickel, B G; Noble, A J; Norman, E B; Oblath, N S; Okada, C E; Ollerhead, R W; Orrell, J L; Oser, S M; Ouellet, C; Peeters, S J M; Poon, A W P; Robertson, B C; Robertson, R G H; Rollin, E; Rosendahl, S S E; Rusu, V L; Schwendener, M H; Simard, O; Simpson, J J; Sims, C J; Sinclair, D; Skensved, P; Smith, M W E; Starinsky, N; Stokstad, R G; Stonehill, L C; Tafirout, R; Takeuchi, Y; Tesić, G; Thomson, M; Thorman, M; Van Berg, R; Van de Water, R G; Virtue, C J; Wall, B L; Waller, D; Waltham, C E; Tseung, H Wan Chan; Wark, D L; West, N; Wilhelmy, J B; Wilkerson, J F; Wilson, J R; Wouters, J M; Yeh, M; Zuber, K

    2004-05-01

    The Sudbury Neutrino Observatory has precisely determined the total active (nu(x)) 8B solar neutrino flux without assumptions about the energy dependence of the nu(e) survival probability. The measurements were made with dissolved NaCl in heavy water to enhance the sensitivity and signature for neutral-current interactions. The flux is found to be 5.21 +/- 0.27(stat)+/-0.38(syst) x 10(6) cm(-2) s(-1), in agreement with previous measurements and standard solar models. A global analysis of these and other solar and reactor neutrino results yields Deltam(2)=7.1(+1.2)(-0.6) x 10(-5) eV(2) and theta=32.5(+2.4)(-2.3) degrees. Maximal mixing is rejected at the equivalent of 5.4 standard deviations.

  2. Parametric Study of a YAV-8B Harrier in Ground Effect using Time-Dependent Navier-Stokes Computations

    NASA Technical Reports Server (NTRS)

    Pandya, Shishir; Chaderjian, Neal; Ahmad, Jasim; Kwak, Dochan (Technical Monitor)

    2002-01-01

    A process is described which enables the generation of 35 time-dependent viscous solutions for a YAV-8B Harrier in ground effect in one week. Overset grids are used to model the complex geometry of the Harrier aircraft and the interaction of its jets with the ground plane and low-speed ambient flow. The time required to complete this parametric study is drastically reduced through the use of process automation, modern computational platforms, and parallel computing. Moreover, a dual-time-stepping algorithm is described which improves solution robustness. Unsteady flow visualization and a frequency domain analysis are also used to identify and correlated key flow structures with the time variation of lift.

  3. Moving-base simulation evaluation of thrust margins for vertical landing for the NASA YAV-8B Harrier aircraft

    NASA Technical Reports Server (NTRS)

    Franklin, James A.; Stortz, Michael W.

    1993-01-01

    A simulation experiment was conducted on Ames Research Center's Vertical Motion Simulator to evaluate the thrust margin for vertical landing required for the YAV-8B Harrier. Two different levels of ground effect were employed, representing the aircraft with or without lift improvement devices installed. In addition, two different inlet temperature profiles were included to cover a wide range of hot gas ingestion. For each ground effect and hot gas ingestion variant, vertical landings were performed at successively heavier weights, with the pilot assessing the acceptability of the operation in each case. Results are presented as a function of hover weight ratio and a metric of the mean ground effect and ingestion that reflect the increase in thrust margin required to provide acceptable control of sink rate during the descent to touchdown with increasing suck down and hot gas ingestion.

  4. Critical assessment of the claim of a significant difference between the results of measurements of the Coulomb dissociation of {sup 8}B and the {sup 7}Be(p,{gamma}){sup 8}B direct capture reaction

    SciTech Connect

    Gai, Moshe

    2006-08-15

    The Coulomb dissociation (CD) of {sup 8}B has emerged as a landmark testing ground of the very method of CD for measuring the cross section of the low-energy {sup 7}Be(p,{gamma}){sup 8}B direct capture (DC) reaction. Recent claims of evidence of slope difference between CD and DC results are critically examined. We include all relevant RIKEN2 data and all previously published DC data, and we examine the extracted so-called average scale-independent slope (b). The parametrization used by the Seattle group to extract the so-called b-slope parameter is also examined at energies above 300 keV. Considering the physical slope (S{sup '}=dS/dE) above 300 keV, we observe a (1.7{sigma}) agreement between slopes (S{sup '}) measured in CD and DC above 300 keV. The claim that S{sub 17}(0) values extracted from CD data are inconsistent and lower than DC results arises from a neglect of substantial systematic uncertainty of low-energy CD data. A consideration of the published CD S{sub 17}(0) results yields very consistent S{sub 17}(0) values that agree with most recent DC measurements. The recent correction of the b-slope parameter suggested by Esbensen, Bertsch, and Snover (EBS) was applied to the wrong b slope calculated using part of the RIKEN2 data. When the correct slope of the RIKEN2 data is used, the EBS correction in fact leads to a substantial disagreement between the slopes of the RIKEN2 data and DC data. In spite of an agreement between CD and DC data neither allow for extracting the slope above 300 keV with high accuracy. Uncertainty of the slope (S{sup '}) leads to an additional uncertainty of the extrapolated S{sub 17}(0). The slope of the astrophysical cross-section factor S{sub 17} must be measured with high precision to enable extraction of the d/s ratio and a high-precision extrapolation of S{sub 17}(0)

  5. 38 CFR 8a.4 - Coverage.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2014-07-01 2014-07-01 false Coverage. 8a.4 Section 8a.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS VETERANS MORTGAGE LIFE INSURANCE § 8a.4 Coverage. (a) The amount of VMLI in force on his or her life at any one time shall...

  6. 38 CFR 8a.4 - Coverage.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2012-07-01 2012-07-01 false Coverage. 8a.4 Section 8a.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS VETERANS MORTGAGE LIFE INSURANCE § 8a.4 Coverage. (a) The amount of VMLI in force on his or her life at any one time shall...

  7. 38 CFR 8a.4 - Coverage.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2013-07-01 2013-07-01 false Coverage. 8a.4 Section 8a.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS VETERANS MORTGAGE LIFE INSURANCE § 8a.4 Coverage. (a) The amount of VMLI in force on his or her life at any one time shall...

  8. 38 CFR 8a.4 - Coverage.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2011-07-01 2011-07-01 false Coverage. 8a.4 Section 8a.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS VETERANS MORTGAGE LIFE INSURANCE § 8a.4 Coverage. (a) The amount of VMLI in force on his or her life at any one time shall...

  9. 38 CFR 8a.4 - Coverage.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false Coverage. 8a.4 Section 8a.4 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS VETERANS MORTGAGE LIFE INSURANCE § 8a.4 Coverage. (a) The amount of VMLI in force on his or her life at any one time shall...

  10. 15 CFR 8a.230 - Transition plans.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Transition plans. 8a.230 Section 8a... Transition plans. (a) Submission of plans. An institution to which § 8a.225 applies and that is composed of more than one administratively separate unit may submit either a single transition plan applicable...

  11. 15 CFR 8a.230 - Transition plans.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Transition plans. 8a.230 Section 8a... Transition plans. (a) Submission of plans. An institution to which § 8a.225 applies and that is composed of more than one administratively separate unit may submit either a single transition plan applicable...

  12. 15 CFR 8a.540 - Advertising.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Advertising. 8a.540 Section 8a.540... in Employment in Education Programs or Activities Prohibited § 8a.540 Advertising. A recipient shall not in any advertising related to employment indicate preference, limitation, specification,...

  13. 15 CFR 8a.515 - Compensation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Compensation. 8a.515 Section 8a.515... in Employment in Education Programs or Activities Prohibited § 8a.515 Compensation. A recipient shall... of pay or other compensation; (b) Results in the payment of wages to employees of one sex at a...

  14. 15 CFR 8a.220 - Admissions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Admissions. 8a.220 Section 8a.220 Commerce and Foreign Trade Office of the Secretary of Commerce NONDISCRIMINATION ON THE BASIS OF SEX IN... institution. (c) Application of §§ 8a.300 through .310. Except as provided in paragraphs (d) and (e) of...

  15. 15 CFR 8a.540 - Advertising.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Advertising. 8a.540 Section 8a.540... in Employment in Education Programs or Activities Prohibited § 8a.540 Advertising. A recipient shall not in any advertising related to employment indicate preference, limitation, specification,...

  16. 15 CFR 8a.510 - Recruitment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Recruitment. 8a.510 Section 8a.510... in Employment in Education Programs or Activities Prohibited § 8a.510 Recruitment. (a) Nondiscriminatory recruitment and hiring. A recipient shall not discriminate on the basis of sex in the...

  17. 15 CFR 8a.310 - Recruitment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Recruitment. 8a.310 Section 8a.310... in Admission and Recruitment Prohibited § 8a.310 Recruitment. (a) Nondiscriminatory recruitment. A... recruitment and admission of students. A recipient may be required to undertake additional recruitment...

  18. 15 CFR 8a.510 - Recruitment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Recruitment. 8a.510 Section 8a.510... in Employment in Education Programs or Activities Prohibited § 8a.510 Recruitment. (a) Nondiscriminatory recruitment and hiring. A recipient shall not discriminate on the basis of sex in the...

  19. A sub-micrometer resolution hard X-ray microprobe system of BL8C at Pohang Light Source.

    PubMed

    Sung, Nark Eon; Lee, Ik Jae; Lee, Kug Seong; Jeong, Seong Hun; Kang, Seen Woong; Shin, Yong Bi

    2015-09-01

    A microprobe system has been installed on the nanoprobe/XAFS beamline (BL8C) at PLS-II, South Korea. Owing to the reproducible switch of the gap of the in-vacuum undulator (IVU), the intense and brilliant hard X-ray beam of an IVU can be used in X-ray fluorescence (XRF) and X-ray absorption fine-structure (XAFS) experiments. For high-spatial-resolution microprobe experiments a Kirkpatrick-Baez mirror system has been used to focus the millimeter-sized X-ray beam to a micrometer-sized beam. The performance of this system was examined by a combination of micro-XRF imaging and micro-XAFS of a beetle wing. These results indicate that the microprobe system of the BL8C can be used to obtain the distributions of trace elements and chemical and structural information of complex materials.

  20. Design and development experience with a digital fly-by-wire control system in an F-8C airplane

    NASA Technical Reports Server (NTRS)

    Deets, D. A.

    1976-01-01

    To assess the feasibility of a digital fly by wire system, the mechanical flight control system of an F-8C airplane was replaced with a digital primary system and an analog backup system. The Apollo computer was used as the heart of the primary system. This paper discusses the experience gained during the design and development of the system and relates it to active control systems that are anticipated for future civil transport applications.

  1. Design and development experience with a digital fly-by-wire control system in an F-8C airplane

    NASA Technical Reports Server (NTRS)

    Deets, D. A.

    1975-01-01

    To assess the feasibility of a digital fly-by-wire system, the mechanical flight control system of an F-8C airplane was replaced with a digital system and an analog backup system. The Apollo computer was used as the heart of the primary system. This paper discusses the experience gained during the design and development of the system and relates it to active control systems that are anticipated for future civil transport applications.

  2. The stochastic control of the F-8C aircraft using the Multiple Model Adaptive Control (MMAC) method

    NASA Technical Reports Server (NTRS)

    Athans, M.; Dunn, K. P.; Greene, E. S.; Lee, W. H.; Sandel, N. R., Jr.

    1975-01-01

    The purpose of this paper is to summarize results obtained for the adaptive control of the F-8C aircraft using the so-called Multiple Model Adaptive Control method. The discussion includes the selection of the performance criteria for both the lateral and the longitudinal dynamics, the design of the Kalman filters for different flight conditions, the 'identification' aspects of the design using hypothesis testing ideas, and the performance of the closed loop adaptive system.

  3. PROCEEDINGS: 1991 INTERNATIONAL CONFERENCE ON MUNICIPAL WASTE COMBUSTION - VOLUME 2. SESSIONS 1B, 2B, 3B, 4B, 7A, 7B, 8A, 8B, AND 9B

    EPA Science Inventory

    The three-volumes document 82 presentations by authors from 15 countries at the Second International Conference on Municipal Waste Combustion (MWC) in Tampa, Florida, April 16-19, 1991. The Conference fostered the exchange of current information on research concerning MWC, ash di...

  4. Small GTPases Rab8a and Rab11a Are Dispensable for Rhodopsin Transport in Mouse Photoreceptors

    PubMed Central

    Ying, Guoxin; Gerstner, Cecilia D.; Frederick, Jeanne M.; Boye, Sanford L.; Hauswirth, William W.; Baehr, Wolfgang

    2016-01-01

    Rab11a and Rab8a are ubiquitous small GTPases shown as required for rhodopsin transport in Xenopus laevis and zebrafish photoreceptors by dominant negative (dn) disruption of function. Here, we generated retina-specific Rab11a (retRab11a) and Rab8a (retRab8a) single and double knockout mice to explore the consequences in mouse photoreceptors. Rhodopsin and other outer segment (OS) membrane proteins targeted correctly to OS and electroretinogram (ERG) responses in all three mutant mouse lines were indistinguishable from wild-type (WT). Further, AAV (adeno-associated virus)-mediated expression of dnRab11b in retRab11a-/- retina, or expression of dnRab8b in retRab8a-/- retina did not cause OS protein mislocalization. Finally, a retRab8a-/- retina injected at one month of age with AAVs expressing dnRab11a, dnRab11b, dnRab8b, and dnRab10 (four dn viruses on Rab8a-/- background) and harvested three months later exhibited normal OS protein localization. In contrast to results obtained with dnRab GTPases in Xenopus and zebrafish, mouse Rab11a and Rab8a are dispensable for proper rhodopsin and outer segment membrane protein targeting. Absence of phenotype after expression of four dn Rab GTPases in a Rab8a-/- retina suggests that Rab8b and Rab11b paralogs maybe dispensable as well. Our data thus demonstrate significant interspecies variation in photoreceptor membrane protein and rhodopsin trafficking. PMID:27529348

  5. {sup 7}Be(p,{gamma}){sup 8}B S factor from ab initio no-core shell model wave functions

    SciTech Connect

    Navratil, P.; Bertulani, C.A.; Caurier, E.

    2006-06-15

    Nuclear structure of {sup 7}Be, {sup 8}B, and {sup 7,8}Li is studied within the ab initio no-core shell model (NCSM). Starting from high-precision nucleon-nucleon (NN) interactions, wave functions of {sup 7}Be and {sup 8}B bound states are obtained in basis spaces up to 10({Dirac_h}/2{pi}){omega} and used to calculate channel cluster form factors (overlap integrals) of the {sup 8}B ground state with {sup 7}Be+p. Due to the use of the harmonic oscillator (HO) basis, the overlap integrals have incorrect asymptotic properties. We fix this problem in two alternative ways. First, by a Woods-Saxon potential solution fit to the interior of the NCSM overlap integrals. Second, by a direct matching with the Whittaker function. The corrected overlap integrals are then used for the {sup 7}Be(p,{gamma}){sup 8}B S-factor calculation. We study the convergence of the S factor with respect to the NCSM HO frequency and the model space size. Our S factor results agree with recent direct measurement data. We also test the spectroscopic factors and the corrected overlap integrals from the NCSM in describing the momentum distributions in knockout reactions with {sup 8}B projectiles. A good agreement with the available experimental data is also found, attesting to the overall consistency of the calculations.

  6. Volumetric Properties of the Mixture Toluene C7H8 + C14H30 Tetradecane (VMSD1212, LB3260_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume A 'Binary Liquid Systems of Nonelectrolytes I' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Toluene C7H8 + C14H30 Tetradecane (VMSD1212, LB3260_V)' providing data by calculation of molar excess volume from low-pressure density measurements at variable mole fraction and constant temperature.

  7. Volumetric Properties of the Mixture Toluene C7H8 + C14H30 Tetradecane (VMSD1111, LB3270_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume A 'Binary Liquid Systems of Nonelectrolytes I' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Toluene C7H8 + C14H30 Tetradecane (VMSD1111, LB3270_V)' providing data from direct low-pressure measurement of mass density at variable mole fraction and constant temperature, in the single-phase region(s).

  8. 15 CFR 8a.300 - Admission.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Admission. 8a.300 Section 8a.300 Commerce and Foreign Trade Office of the Secretary of Commerce NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Admission and Recruitment Prohibited §...

  9. 15 CFR 8a.500 - Employment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Employment. 8a.500 Section 8a.500 Commerce and Foreign Trade Office of the Secretary of Commerce NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs...

  10. 15 CFR 8a.515 - Compensation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Compensation. 8a.515 Section 8a.515 Commerce and Foreign Trade Office of the Secretary of Commerce NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs...

  11. 38 CFR 8a.3 - Effective date.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2013-07-01 2013-07-01 false Effective date. 8a.3 Section 8a.3 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS VETERANS MORTGAGE LIFE... effective August 11, 1971, if on that date, the eligible veteran was obligated under a mortgage loan,...

  12. 38 CFR 8a.3 - Effective date.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2014-07-01 2014-07-01 false Effective date. 8a.3 Section 8a.3 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS VETERANS MORTGAGE LIFE... effective August 11, 1971, if on that date, the eligible veteran was obligated under a mortgage loan,...

  13. 38 CFR 8a.3 - Effective date.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2011-07-01 2011-07-01 false Effective date. 8a.3 Section 8a.3 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS VETERANS MORTGAGE LIFE... effective August 11, 1971, if on that date, the eligible veteran was obligated under a mortgage loan,...

  14. 38 CFR 8a.3 - Effective date.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false Effective date. 8a.3 Section 8a.3 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS VETERANS MORTGAGE LIFE... effective August 11, 1971, if on that date, the eligible veteran was obligated under a mortgage loan,...

  15. 38 CFR 8a.1 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Insurance (VMLI) means the mortgage protection life insurance authorized for veterans under 38 U.S.C. 2106... 8a.1 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS VETERANS MORTGAGE LIFE INSURANCE § 8a.1 Definitions. (a) The term housing unit means a family dwelling or unit, together with...

  16. 15 CFR 8a.500 - Employment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... subjected to discrimination in employment, or recruitment, consideration, or selection therefor, whether... §§ 8a.500 through 8a.550 apply to: (1) Recruitment, advertising, and the process of application for..., layoff, termination, application of nepotism policies, right of return from layoff, and rehiring;...

  17. The effects of ordered carbon vacancies on stability and thermo-mechanical properties of V8C7 compared with VC

    PubMed Central

    Chong, XiaoYu; Jiang, YeHua; Zhou, Rong; Feng, Jing

    2016-01-01

    The ordered non-stoichiometric V8C7 can form in the VCy carbides by the disorder–order phase transformation. The intrusion of ordered carbon vacancies can affect their stability, mechanical, thermal and electronic properties. The relatively thermodynamic stability and mechanical properties at high temperature for the ordered stoichiometric VC and non-stoichiometric V8C7 are investigated in this paper by first-principle calculations combined with the quasi-harmonic approximation. The difference between the properties of VC and V8C7 can be obtained. We find that the V8C7 is thermodynamic more stable than VC, but has weaker elastic heat resistance than VC. Moreover, the minimum thermal conductivity of VC is a little larger than V8C7 and a simple way is proposed to characterize the anisotropy of lattice thermal conductivity based on the Cahill’s model. PMID:27659072

  18. 26 CFR 31.3401(a)(8)(B)-1 - Remuneration for services performed in possession of the United States (other than Puerto Rico...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 15 2010-04-01 2010-04-01 false Remuneration for services performed in... TAX AT SOURCE Collection of Income Tax at Source § 31.3401(a)(8)(B)-1 Remuneration for services...) Remuneration paid for services for an employer (other than the United States or any agency thereof)...

  19. Association between chromosomal aberration of COX8C and tethered spinal cord syndrome: array-based comparative genomic hybridization analysis

    PubMed Central

    Zhao, Qiu-jiong; Bai, Shao-cong; Cheng, Cheng; Tao, Ben-zhang; Wang, Le-kai; Liang, Shuang; Yin, Ling; Hang, Xing-yi; Shang, Ai-jia

    2016-01-01

    Copy number variations have been found in patients with neural tube abnormalities. In this study, we performed genome-wide screening using high-resolution array-based comparative genomic hybridization in three children with tethered spinal cord syndrome and two healthy parents. Of eight copy number variations, four were non-polymorphic. These non-polymorphic copy number variations were associated with Angelman and Prader-Willi syndromes, and microcephaly. Gene function enrichment analysis revealed that COX8C, a gene associated with metabolic disorders of the nervous system, was located in the copy number variation region of Patient 1. Our results indicate that array-based comparative genomic hybridization can be used to diagnose tethered spinal cord syndrome. Our results may help determine the pathogenesis of tethered spinal cord syndrome and prevent occurrence of this disease.

  20. Association between chromosomal aberration of COX8C and tethered spinal cord syndrome: array-based comparative genomic hybridization analysis

    PubMed Central

    Zhao, Qiu-jiong; Bai, Shao-cong; Cheng, Cheng; Tao, Ben-zhang; Wang, Le-kai; Liang, Shuang; Yin, Ling; Hang, Xing-yi; Shang, Ai-jia

    2016-01-01

    Copy number variations have been found in patients with neural tube abnormalities. In this study, we performed genome-wide screening using high-resolution array-based comparative genomic hybridization in three children with tethered spinal cord syndrome and two healthy parents. Of eight copy number variations, four were non-polymorphic. These non-polymorphic copy number variations were associated with Angelman and Prader-Willi syndromes, and microcephaly. Gene function enrichment analysis revealed that COX8C, a gene associated with metabolic disorders of the nervous system, was located in the copy number variation region of Patient 1. Our results indicate that array-based comparative genomic hybridization can be used to diagnose tethered spinal cord syndrome. Our results may help determine the pathogenesis of tethered spinal cord syndrome and prevent occurrence of this disease. PMID:27651783

  1. Performance of Diffusion Aluminide Coatings Applied on Alloy CF8C-Plus at 800oC

    SciTech Connect

    Kumar, Deepak; Dryepondt, Sebastien N; Zhang, Ying; Haynes, James A; Pint, Bruce A; Armstrong, Beth L; Shyam, Amit; Lara-Curzio, Edgar

    2012-01-01

    High performance cast stainless steel, CF8C-Plus, is a low cost alloy with prospective applications ranging from covers and casings of small and medium size gas turbines to turbocharger housing and manifolds in internal combustion engines. Diffusion aluminide coatings were applied on this alloy as a potential strategy for improved oxidation resistance, particularly in wet air and steam. In this paper the performance of the aluminide coatings evaluated by cyclic oxidation experiments in air containing 10 vol.% H2O at 800 C and conventional tension-compression low-cycle-fatigue tests in air at 800 C with a strain range of 0.5% is presented. The results show that specimens coated by a chemical vapor deposition process provide better oxidation resistance than those coated by an Al-slurry coating process. The application of a coating by pack cementation reduced the fatigue life by 15%.

  2. A reliable dual-redundant sensor FDI system for the NASA F8C-DFBW aircraft

    NASA Technical Reports Server (NTRS)

    Deckert, J. C.; Desai, M. N.; Deyst, J. J., Jr.; Willsky, A. S.

    1976-01-01

    In this paper we outline the structure of a sensor failure detection and identification (FDI) system designed for the NASA F8C DFBW aircraft. The system is for use in a dual-redundant environment, and it takes maximal advantage of all functional and kinematic relationships among the sensed variables. The technique is reliable in that we have designed the system to account for a variety of unmodeled effects and have introduced a new concept - the quality sequential probability ratio (QSPR) - that provides a useful measure of our confidence in each test. The methodology used for determining detector parameters and the 'outer loop logic' for combining the various sources of information are described.

  3. Dual redundant sensor FDI techniques applied to the NASA F8C DFBW aircraft. [Failure Detection and Identification

    NASA Technical Reports Server (NTRS)

    Desai, M. N.; Deckert, J. C.; Deyst, J. J.; Willsky, A. S.; Chow, E. Y.

    1976-01-01

    An onboard failure detection and identification (FDI) technique for dual redundant sensors on the NASA F8C digital fly-by-wire (DFBW) aircraft is presented. The failure of one of a pair of sensors of the same type is detected by a direct redundancy trigger which observes the difference between the outputs of these two sensors. Identification of the failed sensor is accomplished utilizing the analytic redundancy that exists as kinematic and functional relationships among the variables being measured by dissimilar instruments. In addition, identification of generic failures, common to both instruments of a given type, is accomplished by using a time trigger to periodically initiate analytic redundancy failure identification tests for individual sensors. The basic form of these tests is the comparison of the measurement of a variable using the suspect instrument with another measurement of the same variable obtained using other instrument types.

  4. Preliminary system design study for a digital fly-by-wire flight control system for an F-8C aircraft

    NASA Technical Reports Server (NTRS)

    Seacord, C. L.; Vaughn, D. K.

    1976-01-01

    The design of a fly-by-wire control system having a mission failure probability of less than one millionth failures per flight hour is examined. Emphasis was placed on developing actuator configurations that would improve the system performance, and consideration of the practical aspects of sensor/computer and computer/actuator interface implementation. Five basic configurations were defined as appropriate candidates for the F-8C research aircraft. Options on the basic configurations were included to cover variations in flight sensors, redundancy levels, data transmission techniques, processor input/output methods, and servo actuator arrangements. The study results can be applied to fly by wire systems for transport aircraft in general and the space shuttle.

  5. NASA/RAE collaboration on nonlinear control using the F-8C digital fly-by-wire aircraft

    NASA Technical Reports Server (NTRS)

    Butler, G. F.; Corbin, M. J.; Mepham, S.; Stewart, J. F.; Larson, R. R.

    1983-01-01

    Design procedures are reviewed for variable integral control to optimize response (VICTOR) algorithms and results of preliminary flight tests are presented. The F-8C aircraft is operated in the remotely augmented vehicle (RAV) mode, with the control laws implemented as FORTRAN programs on a ground-based computer. Pilot commands and sensor information are telemetered to the ground, where the data are processed to form surface commands which are then telemetered back to the aircraft. The RAV mode represents a singlestring (simplex) system and is therefore vulnerable to a hardover since comparison monitoring is not possible. Hence, extensive error checking is conducted on both the ground and airborne computers to prevent the development of potentially hazardous situations. Experience with the RAV monitoring and validation procedures is described.

  6. Association between chromosomal aberration of COX8C and tethered spinal cord syndrome: array-based comparative genomic hybridization analysis.

    PubMed

    Zhao, Qiu-Jiong; Bai, Shao-Cong; Cheng, Cheng; Tao, Ben-Zhang; Wang, Le-Kai; Liang, Shuang; Yin, Ling; Hang, Xing-Yi; Shang, Ai-Jia

    2016-08-01

    Copy number variations have been found in patients with neural tube abnormalities. In this study, we performed genome-wide screening using high-resolution array-based comparative genomic hybridization in three children with tethered spinal cord syndrome and two healthy parents. Of eight copy number variations, four were non-polymorphic. These non-polymorphic copy number variations were associated with Angelman and Prader-Willi syndromes, and microcephaly. Gene function enrichment analysis revealed that COX8C, a gene associated with metabolic disorders of the nervous system, was located in the copy number variation region of Patient 1. Our results indicate that array-based comparative genomic hybridization can be used to diagnose tethered spinal cord syndrome. Our results may help determine the pathogenesis of tethered spinal cord syndrome and prevent occurrence of this disease. PMID:27651783

  7. Identification of fibroblast growth factor-8b target genes associated with early and late cell cycle events in breast cancer cells.

    PubMed

    Nilsson, E M; Brokken, L J S; Narvi, E; Kallio, M J; Härkönen, P L

    2012-07-01

    Fibroblast growth factor-8 (FGF-8) is implicated in the development and progression of breast cancer and its levels are frequently elevated in breast tumors. The mechanisms driving FGF-8-mediated tumorigenesis are not well understood. Herein we aimed to identify target genes associated with FGF-8b-mediated breast cancer cell proliferation by carrying out a cDNA microarray analysis of genes expressed in estrogen receptor negative S115 breast cancer cells treated with FGF-8b for various time periods in comparison with those expressed in non-treated cells. Gene and protein expression was validated for selected genes by qPCR and western blotting respectively. Furthermore, using TRANSBIG data, the expression of human orthologs of FGF-8-regulated genes was correlated to the Nottingham prognostic index and estrogen receptor status. The analysis revealed a number of significantly up- and down-regulated genes in response to FGF-8b at all treatment times. The most differentially expressed genes were genes related to cell cycle regulation, mitosis, cancer, and cell death. Several key regulators of early cell cycle progression such as Btg2 and cyclin D1, as well as regulators of mitosis, including cyclin B, Plk1, survivin, and aurora kinase A, were identified as novel targets for FGF-8b, some of which were additionally shown to correlate with prognosis and ER status in human breast cancer. The results suggest that in stimulation of proliferation FGF-8b not only promotes cell cycle progression through the G1 restriction point but also regulates key proteins involved in chromosomal segregation during mitosis and cytokinesis of breast cancer cells.

  8. Oxidation of Slurry Aluminide Coatings on Cast Stainless Steel Alloy CF8C-Plus at 800oC in Water Vapor

    SciTech Connect

    Haynes, James A; Armstrong, Beth L; Dryepondt, Sebastien N; Kumar, Deepak; Zhang, Ying

    2013-01-01

    A new, cast austenitic stainless steel, CF8C-Plus, has been developed for a wide range of high temperature applications, including diesel exhaust components, turbine casings and turbocharger housings. CF8C-Plus offers significant improvements in creep rupture life and creep rupture strength over standard CF8C steel. However, at higher temperatures and in more aggressive environments, such as those containing significant water vapor, an oxidation-resistant protective coating will be necessary. The oxidation behavior of alloys CF8C and CF8C-Plus with various aluminide coatings were compared at 800oC in air plus 10 vol% water vapor. Due to their affordability, slurry aluminides were the primary coating system of interest, although chemical vapor deposition (CVD) and pack cementation coatings were also compared. Additionally, a preliminary study of the low cycle fatigue behavior of aluminized CF8C-Plus was conducted at 800oC. Each type of coating provided substantial improvements in oxidation behavior, with simple slurry aluminides showing very good oxidation resistance after 4,000 h testing in water vapor. Preliminary low cycle fatigue results indicated that thicker aluminide coatings degraded high temperature fatigue properties of CF8C-Plus, whereas thinner coatings did not. Results suggest that appropriately designed slurry aluminide coatings are a viable option for economical, long-term oxidation protection of austenitic stainless steels in water vapor.

  9. Novel applications for TAZ-8A

    NASA Technical Reports Server (NTRS)

    Riddlebaugh, Stephen M.; Waters, William J.

    1991-01-01

    Recent needs in the non-aerospace industrial sector have revitalized interest in high performance alloys. TAZ-8A has a combination of properties that makes it unique: a high temperature strength, oxidation resistance, abrasion resistance, and exceptional thermal shock resistance. The major drawback for the utilization of this alloy is the relatively high cost compared with the more common iron base alloys. Reduced material consumption and lower costs are possible by using coatings of TAZ-8A on a low cost substrate. Coatings were applied using plasma spray techniques developed by NASA as well as modified plasma vapor deposition (PVD) techniques.

  10. 15 CFR 8a.300 - Admission.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... discriminate against or exclude any person on the basis of pregnancy, childbirth, termination of pregnancy, or...) Subject to § 8a.235(d), shall treat disabilities related to pregnancy, childbirth, termination of pregnancy, or recovery therefrom in the same manner and under the same policies as any other...

  11. 38 CFR 8a.1 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 8a.1 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS VETERANS MORTGAGE LIFE... necessary land therefor, that has been or will be purchased, constructed, or remodeled with a grant to meet... eligible veteran as his or her home, or a family dwelling or unit, including the necessary land...

  12. 15 CFR 8a.230 - Transition plans.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Coverage § 8a.230... order to be approved by the Secretary of Education, a transition plan shall: (1) State the name, address, and Federal Interagency Committee on Education Code of the educational institution submitting...

  13. 15 CFR 8a.105 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Commerce and Foreign Trade Office of the Secretary of Commerce NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Introduction § 8a.105 Definitions..., or matriculation in or at an education program or activity operated by a recipient. Applicant...

  14. 15 CFR 8a.300 - Admission.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... discriminate against or exclude any person on the basis of pregnancy, childbirth, termination of pregnancy, or...) Subject to § 8a.235(d), shall treat disabilities related to pregnancy, childbirth, termination of pregnancy, or recovery therefrom in the same manner and under the same policies as any other...

  15. Heat and corrosion resistant cast CF8C stainless steel with improved high temperature strength and ductility

    DOEpatents

    Maziasz, Philip J.; McGreevy, Tim; Pollard, Michael James; Siebenaler, Chad W.; Swindeman, Robert W.

    2010-08-17

    A CF8C type stainless steel alloy and articles formed therefrom containing about 18.0 weight percent to about 22.0 weight percent chromium and 11.0 weight percent to about 14.0 weight percent nickel; from about 0.05 weight percent to about 0.15 weight percent carbon; from about 2.0 weight percent to about 10.0 weight percent manganese; and from about 0.3 weight percent to about 1.5 weight percent niobium. The present alloys further include less than 0.15 weight percent sulfur which provides high temperature strength both in the matrix and at the grain boundaries without reducing ductility due to cracking along boundaries with continuous or nearly-continuous carbides. The disclosed alloys also have increased nitrogen solubility thereby enhancing strength at all temperatures because nitride precipitates or nitrogen porosity during casting are not observed. The solubility of nitrogen is dramatically enhanced by the presence of manganese, which also retains or improves the solubility of carbon thereby providing additional solid solution strengthening due to the presence of manganese and nitrogen, and combined carbon.

  16. Heat and corrosion resistant cast CF8C stainless steel with improved high temperature strength and ductility

    DOEpatents

    Maziasz, Philip J.; McGreevy, Tim; Pollard, Michael James; Siebenaler, Chad W.; Swindeman, Robert W.

    2006-12-26

    A CF8C type stainless steel alloy and articles formed therefrom containing about 18.0 weight percent to about 22.0 weight percent chromium and 11.0 weight percent to about 14.0 weight percent nickel; from about 0.05 weight percent to about 0.15 weight percent carbon; from about 2.0 weight percent to about 10.0 weight percent manganese; and from about 0.3 weight percent to about 1.5 weight percent niobium. The present alloys further include less than 0.15 weight percent sulfur which provides high temperature strength both in the matrix and at the grain boundaries without reducing ductility due to cracking along boundaries with continuous or nearly-continuous carbides. The disclosed alloys also have increased nitrogen solubility thereby enhancing strength at all temperatures because nitride precipitates or nitrogen porosity during casting are not observed. The solubility of nitrogen is dramatically enhanced by the presence of manganese, which also retains or improves the solubility of carbon thereby providing additional solid solution strengthening due to the presence of manganese and nitrogen, and combined carbon.

  17. Measurement of the rate of nu(e) + d --> p + p + e(-) interactions produced by (8)B solar neutrinos at the Sudbury Neutrino Observatory.

    PubMed

    Ahmad, Q R; Allen, R C; Andersen, T C; Anglin, J D; Bühler, G; Barton, J C; Beier, E W; Bercovitch, M; Bigu, J; Biller, S; Black, R A; Blevis, I; Boardman, R J; Boger, J; Bonvin, E; Boulay, M G; Bowler, M G; Bowles, T J; Brice, S J; Browne, M C; Bullard, T V; Burritt, T H; Cameron, K; Cameron, J; Chan, Y D; Chen, M; Chen, H H; Chen, X; Chon, M C; Cleveland, B T; Clifford, E T; Cowan, J H; Cowen, D F; Cox, G A; Dai, Y; Dai, X; Dalnoki-Veress, F; Davidson, W F; Doe, P J; Doucas, G; Dragowsky, M R; Duba, C A; Duncan, F A; Dunmore, J; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Ferraris, A P; Ford, R J; Fowler, M M; Frame, K; Frank, E D; Frati, W; Germani, J V; Gil, S; Goldschmidt, A; Grant, D R; Hahn, R L; Hallin, A L; Hallman, E D; Hamer, A; Hamian, A A; Haq, R U; Hargrove, C K; Harvey, P J; Hazama, R; Heaton, R; Heeger, K M; Heintzelman, W J; Heise, J; Helmer, R L; Hepburn, J D; Heron, H; Hewett, J; Hime, A; Howe, M; Hykawy, J G; Isaac, M C; Jagam, P; Jelley, N A; Jillings, C; Jonkmans, G; Karn, J; Keener, P T; Kirch, K; Klein, J R; Knox, A B; Komar, R J; Kouzes, R; Kutter, T; Kyba, C C; Law, J; Lawson, I T; Lay, M; Lee, H W; Lesko, K T; Leslie, J R; Levine, I; Locke, W; Lowry, M M; Luoma, S; Lyon, J; Majerus, S; Mak, H B; Marino, A D; McCauley, N; McDonald, A B; McDonald, D S; McFarlane, K; McGregor, G; McLatchie, W; Meijer Drees, R; Mes, H; Mifflin, C; Miller, G G; Milton, G; Moffat, B A; Moorhead, M; Nally, C W; Neubauer, M S; Newcomer, F M; Ng, H S; Noble, A J; Norman, E B; Novikov, V M; O'Neill, M; Okada, C E; Ollerhead, R W; Omori, M; Orrell, J L; Oser, S M; Poon, A W; Radcliffe, T J; Roberge, A; Robertson, B C; Robertson, R G; Rowley, J K; Rusu, V L; Saettler, E; Schaffer, K K; Schuelke, A; Schwendener, M H; Seifert, H; Shatkay, M; Simpson, J J; Sinclair, D; Skensved, P; Smith, A R; Smith, M W; Starinsky, N; Steiger, T D; Stokstad, R G; Storey, R S; Sur, B; Tafirout, R; Tagg, N; Tanner, N W; Taplin, R K; Thorman, M; Thornewell, P; Trent, P T; Tserkovnyak, Y I; Van Berg, R; Van de Water, R G; Virtue, C J; Waltham, C E; Wang, J X; Wark, D L; West, N; Wilhelmy, J B; Wilkerson, J F; Wilson, J; Wittich, P; Wouters, J M; Yeh, M

    2001-08-13

    Solar neutrinos from (8)B decay have been detected at the Sudbury Neutrino Observatory via the charged current (CC) reaction on deuterium and the elastic scattering (ES) of electrons. The flux of nu(e)'s is measured by the CC reaction rate to be straight phi(CC)(nu(e)) = 1.75 +/- 0.07(stat)(+0.12)(-0.11)(syst) +/- 0.05(theor) x 10(6) cm(-2) s(-1). Comparison of straight phi(CC)(nu(e)) to the Super-Kamiokande Collaboration's precision value of the flux inferred from the ES reaction yields a 3.3 sigma difference, assuming the systematic uncertainties are normally distributed, providing evidence of an active non- nu(e) component in the solar flux. The total flux of active 8B neutrinos is determined to be 5.44+/-0.99 x 10(6) cm(-2) s(-1).

  18. Radius studies of 8Li and 8B using the optical-limit Glauber model in conjunction with relativistic mean-field theory

    NASA Astrophysics Data System (ADS)

    Fan, Guang-Wei; Xu, Wang; Fukuda, M.; Pan, Qiang-Yan; Cai, Xiao-Lu; Fan, Gong-Tao; Li, Yong-Jiang; Luo, Wen; Xu, Ben-Ji; Yan, Zhe; Yang, Li-Feng

    2010-10-01

    We study the reaction cross sections (σR) and root-mean-square (RMS) radii of 8Li and 8B, the halo-like nuclei, with stable target 12C, 27Al and 9Be within the standard optical-limit Glauber model, using densities obtained from relativistic mean-field (RMF) formalisms and other types of distributions. It is found that the experimental σR can be reproduced well at high energy. The RMS radius and Δr extracted by RMF-theory and harmonic oscillator distribution are compared. We find that the RMS radius and Δr of 8B are larger than those of 8Li. In addition, we analyze in detail the relationship between σR and density distribution.

  19. Warm Spitzer Observations of Three Hot Exoplanets: XO-4b, HAT-P-6b, and HAT-P-8b

    NASA Astrophysics Data System (ADS)

    Todorov, Kamen O.; Deming, Drake; Knutson, Heather A.; Burrows, Adam; Sada, Pedro V.; Cowan, Nicolas B.; Agol, Eric; Desert, Jean-Michel; Fortney, Jonathan J.; Charbonneau, David; Laughlin, Gregory; Langton, Jonathan; Showman, Adam P.; Lewis, Nikole K.

    2012-02-01

    We analyze Warm Spitzer/Infrared Array Camera observations of the secondary eclipses of three planets, XO-4b, HAT-P-6b, and HAT-P-8b. We measure secondary eclipse amplitudes at 3.6 μm and 4.5 μm for each target. XO-4b exhibits a stronger eclipse depth at 4.5 μm than at 3.6 μm, which is consistent with the presence of a temperature inversion. HAT-P-8b shows a stronger eclipse amplitude at 3.6 μm and is best described by models without a temperature inversion. The eclipse depths of HAT-P-6b can be fitted with models with a small or no temperature inversion. We consider our results in the context of a postulated relationship between stellar activity and temperature inversion and a relationship between irradiation level and planet dayside temperature, as discussed by Knutson et al. and Cowan & Agol, respectively. Our results are consistent with these hypotheses, but do not significantly strengthen them. To measure accurate secondary eclipse central phases, we require accurate ephemerides. We obtain primary transit observations and supplement them with publicly available observations to update the orbital ephemerides of the three planets. Based on the secondary eclipse timing, we set upper boundaries for ecos (ω) for HAT-P-6b, HAT-P-8b, and XO-4b and find that the values are consistent with circular orbits.

  20. WARM SPITZER OBSERVATIONS OF THREE HOT EXOPLANETS: XO-4b, HAT-P-6b, AND HAT-P-8b

    SciTech Connect

    Todorov, Kamen O.; Deming, Drake; Knutson, Heather A.; Burrows, Adam; Sada, Pedro V.; Cowan, Nicolas B.; Agol, Eric; Desert, Jean-Michel; Charbonneau, David; Fortney, Jonathan J.; Laughlin, Gregory; Langton, Jonathan; Showman, Adam P.; Lewis, Nikole K.

    2012-02-10

    We analyze Warm Spitzer/Infrared Array Camera observations of the secondary eclipses of three planets, XO-4b, HAT-P-6b, and HAT-P-8b. We measure secondary eclipse amplitudes at 3.6 {mu}m and 4.5 {mu}m for each target. XO-4b exhibits a stronger eclipse depth at 4.5 {mu}m than at 3.6 {mu}m, which is consistent with the presence of a temperature inversion. HAT-P-8b shows a stronger eclipse amplitude at 3.6 {mu}m and is best described by models without a temperature inversion. The eclipse depths of HAT-P-6b can be fitted with models with a small or no temperature inversion. We consider our results in the context of a postulated relationship between stellar activity and temperature inversion and a relationship between irradiation level and planet dayside temperature, as discussed by Knutson et al. and Cowan and Agol, respectively. Our results are consistent with these hypotheses, but do not significantly strengthen them. To measure accurate secondary eclipse central phases, we require accurate ephemerides. We obtain primary transit observations and supplement them with publicly available observations to update the orbital ephemerides of the three planets. Based on the secondary eclipse timing, we set upper boundaries for ecos ({omega}) for HAT-P-6b, HAT-P-8b, and XO-4b and find that the values are consistent with circular orbits.

  1. Distinction between elastic scattering of weakly bound proton- and neutron-rich nuclei: The case of 8B and 11Be

    NASA Astrophysics Data System (ADS)

    Yang, Y. Y.; Liu, X.; Pang, D. Y.

    2016-09-01

    Experimental data show that the elastic scattering cross sections of the neutron-rich nucleus 11Be are greatly reduced by the coupling effects from the breakup channels, while those of the proton-rich nucleus 8B are not. Such difference is found to persist in results of systematic calculations of 8B elastic scattering from 208Pb at 60 and 170.3 MeV and from 64Zn at 32 and 86 MeV, and 11Be elastic scattering from 208Pb at 55 and 143 MeV and from 64Zn at 29 and 66 MeV with the continuum-discretized coupled channel (CDCC) method. The Coulomb and centrifugal barriers experienced by the valence proton in the ground state of 8B, which do not exist for the valence neutron in the ground state of 11Be, are found to be the reason for such differences in the angular distributions of elastic scattering cross sections of these two weakly bound nuclei.

  2. Improving a recombinant Zymomonas mobilis strain 8b through continuous adaptation on dilute acid pretreated corn stover hydrolysate

    SciTech Connect

    Mohagheghi, Ali; Linger, Jeffrey G.; Yang, Shihui; Smith, Holly; Dowe, Nancy; Zhang, Min; Pienkos, Philip T.

    2015-03-31

    Complete conversion of the major sugars of biomass including both the C5 and C6 sugars is critical for biofuel production processes. Several inhibitory compounds like acetate, hydroxymethylfurfural (HMF), and furfural are produced from the biomass pretreatment process leading to ‘hydrolysate toxicity,’ a major problem for microorganisms to achieve complete sugar utilization. Therefore, development of more robust microorganisms to utilize the sugars released from biomass under toxic environment is critical. In this study, we use continuous culture methodologies to evolve and adapt the ethanologenic bacterium Zymomonas mobilis to improve its ethanol productivity using corn stover hydrolysate. The results are the following: A turbidostat was used to adapt the Z. mobilis strain 8b in the pretreated corn stover liquor. The adaptation was initiated using pure sugar (glucose and xylose) followed by feeding neutralized liquor at different dilution rates. Once the turbidostat reached 60% liquor content, the cells began washing out and the adaptation was stopped. Several ‘sub-strains’ were isolated, and one of them, SS3 (sub-strain 3), had 59% higher xylose utilization than the parent strain 8b when evaluated on 55% neutralized PCS (pretreated corn stover) liquor. Using saccharified PCS slurry generated by enzymatic hydrolysis from 25% solids loading, SS3 generated an ethanol yield of 75.5% compared to 64% for parent strain 8b. Furthermore, the total xylose utilization was 57.7% for SS3 versus 27.4% for strain 8b. To determine the underlying genotypes in these new sub-strains, we conducted genomic resequencing and identified numerous single-nucleotide mutations (SNPs) that had arisen in SS3. We further performed quantitative reverse transcription PCR (qRT-PCR) on genes potentially affected by these SNPs and identified significant down-regulation of two genes, ZMO0153 and ZMO0776, in SS3 suggesting potential genetic mechanisms behind SS3

  3. Creep and Oxidation Behavior of Modified CF8C-Plus with W, Cu, Ni, and Cr

    NASA Astrophysics Data System (ADS)

    Unocic, Kinga A.; Dryepondt, Sebastien; Yamamoto, Yukinori; Maziasz, Philip J.

    2016-04-01

    The microstructures of modified CF8C-Plus (Fe-19Cr-12Ni-0.4W-3.8Mn-0.2Mo-0.6Nb-0.5Si-0.9C) with W and Cu (CF8CPWCu) and CF8CPWCu enhanced with 21Cr + 15Ni or 22Cr + 17.5Ni were characterized in the as-cast condition and after creep testing. When imaged at lower magnifications, the as-cast microstructure was similar among all three alloys as they all contained a Nb-rich interdendritic phase and Mn-based inclusions. Transmission electron microscopy (TEM) analysis showed the presence of nanoscale Cu-rich nanoprecipitates distributed uniformly throughout the matrix of CF8CPWCu, whereas in CF8CPWCu22/17, Cu precipitates were found primarily at the grain boundaries. The presence of these nanoscale Cu-rich particles, in addition to W-rich Cr23C6, nanoscale Nb carbides, and Z-phase (Nb2Cr2N2), improved the creep strength of the CF8CPWCu steel. Modification of CF8CPWCu with Cr and Ni contents slightly decreased the creep strength but significantly improved the oxidation behavior at 1073 K (800 °C). In particular, the addition of 22Cr and 17.5Ni strongly enhanced the oxidation resistance of the stainless steel resulting in a 100 degrees or greater temperature improvement, and this composition provided the best balance between improving both mechanical properties and oxidation resistance.

  4. Creep and oxidation behavior of modified CF8C-plus with W, Cu, Ni, and Cr

    DOE PAGES

    Unocic, Kinga A.; Dryepondt, Sebastien N.; Yamamoto, Yukinori; Maziasz, Philip J.

    2016-02-01

    Here, the microstructures of modified CF8C-Plus (Fe-19Cr-12Ni-0.4W-3.8Mn-0.2Mo-0.6Nb-0.5Si-0.9C) with W and Cu (CF8CPWCu) and CF8CPWCu enhanced with 21Cr + 15Ni or 22Cr + 17.5Ni were characterized in the as-cast condition and after creep testing. When imaged at lower magnifications, the as-cast microstructure was similar among all three alloys as they all contained a Nb-rich interdendritic phase and Mn-based inclusions. Transmission electron microscopy (TEM) analysis showed the presence of nanoscale Cu-rich nanoprecipitates distributed uniformly throughout the matrix of CF8CPWCu, whereas in CF8CPWCu22/17, Cu precipitates were found primarily at the grain boundaries. The presence of these nanoscale Cu-rich particles, in addition to W-richmore » Cr23C6, nanoscale Nb carbides, and Z-phase (Nb2Cr2N2), improved the creep strength of the CF8CPWCu steel. Modification of CF8CPWCu with Cr and Ni contents slightly decreased the creep strength but significantly improved the oxidation behavior at 1073 K (800 °C). In particular, the addition of 22Cr and 17.5Ni strongly enhanced the oxidation resistance of the stainless steel resulting in a 100 degrees or greater temperature improvement, and this composition provided the best balance between improving both mechanical properties and oxidation resistance.« less

  5. Optimized anisotropic magnetoelectric response of Fe61.6Co16.4Si10.8B11.2/PVDF/Fe61.6Co16.4Si10.8B11.2 laminates for AC/DC magnetic field sensing

    NASA Astrophysics Data System (ADS)

    Reis, S.; Silva, M. P.; Castro, N.; Correia, V.; Gutierrez, J.; Lasheras, A.; Lanceros-Mendez, S.; Martins, P.

    2016-05-01

    The anisotropic magnetoelectric (ME) effect on a Fe61.6Co16.4Si10.8B11.2/PVDF Fe61.6Co16.4Si10.8B11.2 laminate composite has been used for the development of a magnetic field sensor able to detect both the magnitude and direction of AC and DC magnetic fields. The accuracy (99% for both AC and DC sensors), linearity (92% for the DC sensor and 99% for the AC sensor) and reproducibility (99% for both sensors) indicate the suitability of the sensor for applications. Furthermore, the sensitivity of the Fe61.6Co16.4Si10.8B11.2/PVDF/Fe61.6Co16.4Si10.8B11.2 anisotropic magnetic sensor—15 and 1400 mV Oe-1 for the DC and AC fields, respectively—are the highest reported in the literature for polymer-based ME materials. Such features, combined with its flexibility, versatility, light weight, low cost and low-temperature fabrication, lead to the suitability of the developed sensor for use in magnetic sensor applications.

  6. Identification, characterization and genetic mapping of TLR7, TLR8a1 and TLR8a2 genes in rainbow trout (Oncorhynchus mykiss)

    USGS Publications Warehouse

    Palti, Yniv; Gahr, Scott A.; Purcell, Maureen K.; Hadidi, Sima; Rexroad, Caird E.; Wiens, Gregory A.

    2010-01-01

    Induction of the innate immune pathways is critical for early anti-viral defense but there is limited understanding of how teleost fish recognize viral molecules and activate these pathways. In mammals, Toll-like receptors (TLR) 7 and 8 bind single-stranded RNA of viral origin and are activated by synthetic anti-viral imidazoquinoline compounds. Herein, we identify and describe the rainbow trout (Oncorhynchus mykiss) TLR7 and TLR8 gene orthologs and their mRNA expression. Two TLR7/8 loci were identified from a rainbow trout bacterial artificial chromosome (BAC) library using DNA fingerprinting and genetic linkage analyses. Direct sequencing of two representative BACs revealed intact omTLR7 and omTLR8a1 open reading frames (ORFs) located on chromosome 3 and a second locus on chromosome 22 that contains an omTLR8a2 ORF and a putative TLR7 pseudogene. We used the omTLR8a1/2 nomenclature for the two trout TLR8 genes as phylogenetic analysis revealed that they and all the other teleost TLR8 genes sequenced to date are similar to the zebrafish TLR8a, but are distinct from the zebrafish TLR8b. The duplicated trout loci exhibit conserved synteny with other fish genomes extending beyond the tandem of TLR7/8 genes. The trout TLR7 and 8a1/2 genes are composed of a single large exon similar to all other described TLR7/8 genes. The omTLR7 ORF is predicted to encode a 1049 amino acid (aa) protein with 84% similarity to the Fugu TLR7 and a conserved pattern of predicted leucine-rich repeats (LRR). The omTLR8a1 and omTLR8a2 are predicted to encode 1035- and 1034-aa proteins, respectively, and have 86% similarity to each other. omTLR8a1 is likely the ortholog of the only Atlantic salmon TLR8 gene described to date as they have 95% aa sequence similarity. The tissue expression profiles of omTLR7, omTLR8a1 and omTLR8a2 in healthy trout were highest in spleen tissue followed by anterior and then posterior kidney tissues. Rainbow trout anterior kidney leukocytes produced elevated

  7. Identification, characterization and genetic mapping of TLR7, TLR8a1 and TLR8a2 genes in rainbow trout (Oncorhynchus mykiss).

    PubMed

    Palti, Yniv; Gahr, Scott A; Purcell, Maureen K; Hadidi, Sima; Rexroad, Caird E; Wiens, Gregory D

    2010-02-01

    Induction of the innate immune pathways is critical for early anti-viral defense but there is limited understanding of how teleost fish recognize viral molecules and activate these pathways. In mammals, Toll-like receptors (TLR) 7 and 8 bind single-stranded RNA of viral origin and are activated by synthetic anti-viral imidazoquinoline compounds. Herein, we identify and describe the rainbow trout (Oncorhynchus mykiss) TLR7 and TLR8 gene orthologs and their mRNA expression. Two TLR7/8 loci were identified from a rainbow trout bacterial artificial chromosome (BAC) library using DNA fingerprinting and genetic linkage analyses. Direct sequencing of two representative BACs revealed intact omTLR7 and omTLR8a1 open reading frames (ORFs) located on chromosome 3 and a second locus on chromosome 22 that contains an omTLR8a2 ORF and a putative TLR7 pseudogene. We used the omTLR8a1/2 nomenclature for the two trout TLR8 genes as phylogenetic analysis revealed that they and all the other teleost TLR8 genes sequenced to date are similar to the zebrafish TLR8a, but are distinct from the zebrafish TLR8b. The duplicated trout loci exhibit conserved synteny with other fish genomes extending beyond the tandem of TLR7/8 genes. The trout TLR7 and 8a1/2 genes are composed of a single large exon similar to all other described TLR7/8 genes. The omTLR7 ORF is predicted to encode a 1049 amino acid (aa) protein with 84% similarity to the Fugu TLR7 and a conserved pattern of predicted leucine-rich repeats (LRR). The omTLR8a1 and omTLR8a2 are predicted to encode 1035- and 1034-aa proteins, respectively, and have 86% similarity to each other. omTLR8a1 is likely the ortholog of the only Atlantic salmon TLR8 gene described to date as they have 95% aa sequence similarity. The tissue expression profiles of omTLR7, omTLR8a1 and omTLR8a2 in healthy trout were highest in spleen tissue followed by anterior and then posterior kidney tissues. Rainbow trout anterior kidney leukocytes produced elevated

  8. Warm Spitzer Secondary Transit Photometry of Hot Jupiters HAT-P-6b, HAT-P-8b and XO-4b

    NASA Astrophysics Data System (ADS)

    Todorov, Kamen 0.; Deming, D.; Knutson, H.; Burrows, A.; Sada, P.; Agol, E.; Desert, J.; Fortney, J. J.; Charbonneau, D.; Cowan, N. B.; Laughlin, G.; Langton, J.; Showman, A. P.; Lewis, N. K.

    2011-05-01

    An increasing number of transiting exoplanets have been observed at secondary eclipse. By measuring the depth of these eclipses at different wavelengths it is possible to distinguish between planets that have a temperature inversion in the upper layers of their atmospheres and ones that do not. We observed XO-4b, HAT-P-6b and HAT-P-8b during secondary eclipse with the IRAC instrument on Warm Spitzer at 3.6 and 4.5 microns. We compare the resulting eclipse depths to atmospheric models with and without temperature inversions, and thereby place constraints on the properties of their day-side atmospheres and heat redistribution efficiencies. The XO-4b and HAT-P-6b eclipse depths agree best with inverted models, while HAT-P-8b exhibits no temperature inversion. Knutson et al. (2010) hypothesized a correlation between lack of a temperature inversion and host star activity. Also, Cowan & Agol (2011), investigated the dependence between planetary effective temperatures, assuming no redistribution, and heat redistribution efficiency, finding that the hottest planets re-distribute heat inefficiently. We compare our planets with the Knutson and Cowan-Agol relations, and we find that they are consistent with the Knutson et al. activity hypothesis, but they are not hot enough to test the Cowan & Agol hypothesis.

  9. Corrosion Behaviour of Ni{sub 78}Si{sub 8}B{sub 14} Alloy in 1 M NaCl Aqueous Medium at Room Temperature

    SciTech Connect

    Mathur, Shubhra; Vyas, Rishi; Sachdev, K.; Sharma, S. K.

    2010-06-29

    Potentiodynamic polarization studies were performed on the air side and wheel side of the specimen Ni{sub 78}Si{sub 8}B{sub 14} in 1 M NaCl aqueous medium at room temperature. It was observed that the corrosion current density on the air side of the specimen was less than that of the wheel side of the specimen in 1 M NaCl aqueous medium. The amorphous nature of the air side and wheel side of the specimen Ni{sub 78}Si{sub 8}B{sub 14} was confirmed by X-ray diffraction (XRD). The observed behaviour can be related to the surface morphology due to processing of the ribbons. It is suggested that the inferior corrosion resistance of the wheel side surface is attributed to higher concentration of quenched-in defects due to higher cooling rate. Weight loss studies were also carried out in order to corroborate the polarization results. The results are discussed in the paper in the light of data reported in the literature.

  10. Measurement of the nue and Total 8B Solar Neutrino Fluxes with theSudbury Neutrino Observatory Phase I Data Set

    SciTech Connect

    Aharmim, B.; Ahmad, Q.R.; Ahmed, S.N.; Allen, R.C.; Andersen,T.C.; Anglin, J.D.; Buehler, G.; Barton, J.C.; Beier, E.W.; Bercovitch,M.; Bergevin, M.; Bigu, J.; Biller, S.D.; Black, R.A.; Blevis, I.; Boardman, R.J.; Boger, J.; Bonvin, E.; Boulay, M.G.; Bowler, M.G.; Bowles, T.J.; Brice, S.J.; Browne, M.C.; Bullard, T.V.; Burritt, T.H.; Cameron, J.; Chan, Y.D.; Chen, H.H.; Chen, M.; Chen, X.; Cleveland, B.T.; Cowan, J.H.M.; Cowen, D.F.; Cox, G.A.; Currat, C.A.; Dai, X.; Dalnoki-Veress, F.; Davidson, W.F.; Deng, H.; DiMarco, M.; Doe, P.J.; Doucas, G.; Dragowsky, M.R.; Duba, C.A.; Duncan, F.A.; Dunford, M.; Dunmore, J.A.; Earle, E.D.; Elliott, S.R.; Evans, H.C.; Ewan, G.T.; Farine, J.; Fergani, H.; Ferraris, A.P.; Fleurot, F.; Ford, R.J.; Formaggio, J.A.; Fowler, M.M.; Frame, K.; Frank, E.D.; Frati, W.; Gagnon,N.; Germani, J.V.; Gil, S.; Goldschmidt, A.; Goon, J.T.M.; Graham, K.; Grant, D.R.; Guillian, E.; Hahn, R.L.; Hallin, A.L.; Hallman, E.D.; Hamer, A.S.; Hamian, A.A.; Handler, W.B.; Haq, R.U.; Hargrove, C.K.; Harvey, P.J.; Hazama, R.; Heeger, K.M.; Heintzelman, W.J.; Heise, J.; Helmer, R.L.; Henning, R.; Hepburn, J.D.; Heron, H.; Hewett, J.; Hime,A.; Howard, C.; Howe, M.A.; Huang, M.; Hykawy, J.G.; Isaac, M.C.P.; Jagam, P.; Jamieson, B.; Jelley, N.A.; Jillings, C.; Jonkmans, G.; Kazkaz, K.; Keener, P.T.; Kirch, K.; Klein, J.R.; Knox, A.B.; Komar,R.J.; Kormos, L.L.; Kos, M.; Kouzes, R.; Krueger, A.; Kraus, C.; Krauss,C.B.; Kutter, T.; Kyba, C.C.M.; Labranche, H.; Lange, R.; Law, J.; Lawson, I.T.; Lay, M.; Lee, H.W.; Lesko, K.T.; Leslie, J.R.; Levine, I.; Loach, J.C.; Locke, W.; Luoma, S.; Lyon, J.; MacLellan, R.; Majerus, S.; Mak, H.B.; Maneira, J.; Marino, A.D.; Martin, R.; McCauley, N.; McDonald,A.B.; McDonald, D.S.; McFarlane, K.; McGee, S.; McGregor, G.; MeijerDrees, R.; Mes, H.; Mifflin, C.; Miknaitis, K.K.S.; Miller, M.L.; Milton,G.; Moffat, B.A.; Monreal, B.; Moorhead, M.; Morrissette, B.; Nally,C.W.; Neubauer, M.S.; et al.

    2007-02-01

    This article provides the complete description of resultsfrom the Phase I data set of the Sudbury Neutrino Observatory (SNO). ThePhase I data set is based on a 0.65 kt-year exposure of heavy water tothe solar 8B neutrino flux. Included here are details of the SNO physicsand detector model, evaluations of systematic uncertainties, andestimates of backgrounds. Also discussed are SNO's approach tostatistical extraction of the signals from the three neutrino reactions(charged current, neutral current, and elastic scattering) and theresults of a search for a day-night asymmetry in the ?e flux. Under theassumption that the 8B spectrum is undistorted, the measurements fromthis phase yield a solar ?e flux of ?(?e) =1.76+0.05?0.05(stat.)+0.09?0.09 (syst.) x 106 cm?2 s?1, and a non-?ecomponent ?(? mu) = 3.41+0.45?0.45(stat.)+0.48?0.45 (syst.) x 106 cm?2s?1. The sum of these components provides a total flux in excellentagreement with the predictions of Standard Solar Models. The day-nightasymmetry in the ?e flux is found to be Ae = 7.0 +- 4.9 (stat.)+1.3?1.2percent (sys.), when the asymmetry in the total flux is constrained to bezero.

  11. Identification of a key recombinant narrows the CADASIL gene region to 8 cM and argues against allelism of CADASIL and familial hemiplegic migraine

    SciTech Connect

    Dichgans, M.; Mayer, M.; Straube, A.

    1996-02-15

    This article reports on new information regarding the genetic mapping of the human CADASIL gene region. Previously, the gene had been mapped to human chromosome 19q12. Using the identification of a chromosomal crossover, the region has been refined to an 8-cM interval. 11 refs., 2 figs., 1 tab.

  12. 26 CFR 31.3401(a)(8)(C)-1 - Remuneration for services performed in Puerto Rico by citizen of the United States.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 15 2010-04-01 2010-04-01 false Remuneration for services performed in Puerto... Source § 31.3401(a)(8)(C)-1 Remuneration for services performed in Puerto Rico by citizen of the United States. (a) Remuneration paid for services performed within Puerto Rico for an employer (other than...

  13. Ground and flight test experience with a triple redundant digital fly by wire control system. [installed in F-8C aircraft

    NASA Technical Reports Server (NTRS)

    Jarvis, C. R.; Szalai, K. J.

    1981-01-01

    A triplex digital fly by wire flight control system was developed and installed in an F-8C aircraft to provide fail operative, full authority control. Hardware and software redundancy management techniques were designed to detect and identify failures in the system. Control functions typical of those projected for future actively controlled vehicles were implemented.

  14. Antimicrobial and anti-biofilm effect of Bac8c on major bacteria associated with dental caries and Streptococcus mutans biofilms.

    PubMed

    Ding, Yonglin; Wang, Wei; Fan, Meng; Tong, Zhongchun; Kuang, Rong; Jiang, WenKai; Ni, Longxing

    2014-02-01

    Dental caries is a common oral bacterial infectious disease. Its prevention and treatment requires control of the causative pathogens within dental plaque, especially Streptococcus mutans (S. mutans). Antimicrobial peptides (AMPs), one of the promising substitutes for conventional antibiotics, have been widely tested and used for controlling bacterial infections. The present study focuses on evaluating the potential of the novel AMPs cyclic bactenecin and its derivatives against bacteria associated with dental caries. The results indicate that Bac8c displayed highest activity against the bacteria tested, whereas both cyclic and linear bactenecin had weak antimicrobial activity. The cytotoxicity assay showed that Bac8c did not cause detectable toxicity at concentrations of 32-128μg/ml for 5min or 32-64μg/ml for 60min. S. mutans and Lactobacillus fermenti treated with Bac8c showed variable effects on bacterial structure via scanning electron microscopy and transmission electron microscopy. There appeared to be a large amount of extracellular debris and obvious holes on the cell surface, as well as loss of cell wall and nucleoid condensation. The BioFlux system was employed to generate S. mutans biofilms under a controlled flow, which more closely resemble the formation process of natural biofilms. Bac8c remarkably reduced the viability of cells in biofilms formed in the BioFlux system. This phenomenon was further analyzed and verified by real-time PCR results of a significant suppression of the genes involved in S. mutans biofilm formation. Taken together, this study suggests that Bac8c has a potential clinical application in preventing and treating dental caries.

  15. Synthesis, structural elucidation and spectroscopic analysis of 3a,8b-dihydroxy-4-oxo-1H,2H,3H,3aH,4H,8bH-indeno[1,2-d]imidazolidin-2-iminium chloride

    NASA Astrophysics Data System (ADS)

    Uma Devi, T.; Priya, S.; Selvanayagam, S.; Ravikumar, K.; Anitha, K.

    2012-11-01

    Ninhydrin guanidinium chloride (3a,8b-dihydroxy-4-oxo-1H,2H,3H,3aH,4H,8bH-indeno [1,2-d]imidazolidin-2-iminium chloride) a semiorganic crystal was synthesized. The structure was determined using X-ray single crystal technique. Comparisons between the FT-IR spectrum of ninhydrin guanidinium chloride with ninhydrin were made. Melting point was found using thermal measurements. The molecular geometry, vibrational frequencies and Mulliken charges of the compound in the ground state have been calculated by the density functional theory (DFT) method with 3-21G(d,p) basis set and theoretical frequencies were compared with the experimental FT-IR spectrum. Besides, molecular electrostatic potential (MEP), frontier molecular orbitals (FMO) analysis, natural bond orbitals (NBO) and thermodynamic properties at various temperatures of the compound were investigated by theoretical calculations.

  16. Independent measurement of the total active 8B solar neutrino flux using an array of 3He proportional counters at the Sudbury Neutrino Observatory.

    PubMed

    Aharmim, B; Ahmed, S N; Amsbaugh, J F; Anthony, A E; Banar, J; Barros, N; Beier, E W; Bellerive, A; Beltran, B; Bergevin, M; Biller, S D; Boudjemline, K; Boulay, M G; Bowles, T J; Browne, M C; Bullard, T V; Burritt, T H; Cai, B; Chan, Y D; Chauhan, D; Chen, M; Cleveland, B T; Cox-Mobrand, G A; Currat, C A; Dai, X; Deng, H; Detwiler, J; DiMarco, M; Doe, P J; Doucas, G; Drouin, P-L; Duba, C A; Duncan, F A; Dunford, M; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Ford, R J; Formaggio, J A; Fowler, M M; Gagnon, N; Germani, J V; Goldschmidt, A; Goon, J T M; Graham, K; Guillian, E; Habib, S; Hahn, R L; Hallin, A L; Hallman, E D; Hamian, A A; Harper, G C; Harvey, P J; Hazama, R; Heeger, K M; Heintzelman, W J; Heise, J; Helmer, R L; Henning, R; Hime, A; Howard, C; Howe, M A; Huang, M; Jagam, P; Jamieson, B; Jelley, N A; Keeter, K J; Klein, J R; Kormos, L L; Kos, M; Krüger, A; Kraus, C; Krauss, C B; Kutter, T; Kyba, C C M; Lange, R; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Loach, J C; MacLellan, R; Majerus, S; Mak, H B; Maneira, J; Martin, R; McBryde, K; McCauley, N; McDonald, A B; McGee, S; Mifflin, C; Miller, G G; Miller, M L; Monreal, B; Monroe, J; Morissette, B; Myers, A; Nickel, B G; Noble, A J; Oblath, N S; O'Keeffe, H M; Ollerhead, R W; Gann, G D Orebi; Oser, S M; Ott, R A; Peeters, S J M; Poon, A W P; Prior, G; Reitzner, S D; Rielage, K; Robertson, B C; Robertson, R G H; Rollin, E; Schwendener, M H; Secrest, J A; Seibert, S R; Simard, O; Simpson, J J; Sinclair, L; Skensved, P; Smith, M W E; Steiger, T D; Stonehill, L C; Tesić, G; Thornewell, P M; Tolich, N; Tsui, T; Tunnell, C D; Van Wechel, T; Van Berg, R; VanDevender, B A; Virtue, C J; Walker, T J; Wall, B L; Waller, D; Tseung, H Wan Chan; Wendland, J; West, N; Wilhelmy, J B; Wilkerson, J F; Wilson, J R; Wouters, J M; Wright, A; Yeh, M; Zhang, F; Zuber, K

    2008-09-12

    The Sudbury Neutrino Observatory (SNO) used an array of 3He proportional counters to measure the rate of neutral-current interactions in heavy water and precisely determined the total active (nu_x) 8B solar neutrino flux. This technique is independent of previous methods employed by SNO. The total flux is found to be 5.54_-0.31;+0.33(stat)-0.34+0.36(syst)x10(6) cm(-2) s(-1), in agreement with previous measurements and standard solar models. A global analysis of solar and reactor neutrino results yields Deltam2=7.59_-0.21;+0.19x10(-5) eV2 and theta=34.4_-1.2;+1.3 degrees. The uncertainty on the mixing angle has been reduced from SNO's previous results.

  17. Measurement of the νe and total 8B solar neutrino fluxes with the Sudbury Neutrino Observatory phase-III data set

    NASA Astrophysics Data System (ADS)

    Aharmim, B.; Ahmed, S. N.; Amsbaugh, J. F.; Anaya, J. M.; Anthony, A. E.; Banar, J.; Barros, N.; Beier, E. W.; Bellerive, A.; Beltran, B.; Bergevin, M.; Biller, S. D.; Boudjemline, K.; Boulay, M. G.; Bowles, T. J.; Browne, M. C.; Bullard, T. V.; Burritt, T. H.; Cai, B.; Chan, Y. D.; Chauhan, D.; Chen, M.; Cleveland, B. T.; Cox, G. A.; Currat, C. A.; Dai, X.; Deng, H.; Detwiler, J. A.; DiMarco, M.; Doe, P. J.; Doucas, G.; Dragowsky, M. R.; Drouin, P.-L.; Duba, C. A.; Duncan, F. A.; Dunford, M.; Earle, E. D.; Elliott, S. R.; Evans, H. C.; Ewan, G. T.; Farine, J.; Fergani, H.; Fleurot, F.; Ford, R. J.; Formaggio, J. A.; Fowler, M. M.; Gagnon, N.; Germani, J. V.; Goldschmidt, A.; Goon, J. TM.; Graham, K.; Guillian, E.; Habib, S.; Hahn, R. L.; Hallin, A. L.; Hallman, E. D.; Hamian, A. A.; Harper, G. C.; Harvey, P. J.; Hazama, R.; Heeger, K. M.; Heintzelman, W. J.; Heise, J.; Helmer, R. L.; Henning, R.; Hime, A.; Howard, C.; Howe, M. A.; Huang, M.; Jagam, P.; Jamieson, B.; Jelley, N. A.; Keeter, K. J.; Klein, J. R.; Kormos, L. L.; Kos, M.; Krüger, A.; Kraus, C.; Krauss, C. B.; Kutter, T.; Kyba, C. C. M.; Lange, R.; Law, J.; Lawson, I. T.; Lesko, K. T.; Leslie, J. R.; Loach, J. C.; MacLellan, R.; Majerus, S.; Mak, H. B.; Maneira, J.; Martin, R.; McCauley, N.; McDonald, A. B.; McGee, S. R.; Mifflin, C.; Miller, G. G.; Miller, M. L.; Monreal, B.; Monroe, J.; Morissette, B.; Myers, A. W.; Nickel, B. G.; Noble, A. J.; O'Keeffe, H. M.; Oblath, N. S.; Ollerhead, R. W.; Orebi Gann, G. D.; Oser, S. M.; Ott, R. A.; Peeters, S. J. M.; Poon, A. W. P.; Prior, G.; Reitzner, S. D.; Rielage, K.; Robertson, B. C.; Robertson, R. G. H.; Rollin, E.; Schwendener, M. H.; Secrest, J. A.; Seibert, S. R.; Simard, O.; Simpson, J. J.; Skensved, P.; Smith, M. W. E.; Sonley, T. J.; Steiger, T. D.; Stonehill, L. C.; Tešić, G.; Thornewell, P. M.; Tolich, N.; Tsui, T.; Tunnell, C. D.; Van Wechel, T.; Van Berg, R.; VanDevender, B. A.; Virtue, C. J.; Wall, B. L.; Waller, D.; Wan Chan Tseung, H.; Wendland, J.; West, N.; Wilhelmy, J. B.; Wilkerson, J. F.; Wilson, J. R.; Wouters, J. M.; Wright, A.; Yeh, M.; Zhang, F.; Zuber, K.

    2013-01-01

    This paper details the solar neutrino analysis of the 385.17-day phase-III data set acquired by the Sudbury Neutrino Observatory (SNO). An array of 3He proportional counters was installed in the heavy-water target to measure precisely the rate of neutrino-deuteron neutral-current interactions. This technique to determine the total active 8B solar neutrino flux was largely independent of the methods employed in previous phases. The total flux of active neutrinos was measured to be 5.54-0.31+0.33(stat.)-0.34+0.36(syst.)×106 cm-2 s-1, consistent with previous measurements and standard solar models. A global analysis of solar and reactor neutrino mixing parameters yielded the best-fit values of Δm2=7.59-0.21+0.19×10-5eV2 and θ=34.4-1.2+1.3degrees.

  18. Navier-Stokes simulation of external/internal transonic flow on the forebody/inlet of the AV-8B Harrier II

    NASA Technical Reports Server (NTRS)

    Mysko, Stephen J.; Chyu, Wei J.; Stortz, Michael W.; Chow, Chuen-Yen

    1993-01-01

    In this work, the computation of combined external/internal transonic flow on the complex forebody/inlet configuration of the AV-8B Harrier II is performed. The actual aircraft has been measured and its surface and surrounding domain, in which the fuselage and inlet have a common wall, have been described using structured grids. The 'thin-layer' Navier-Stokes equations were used to model the flow along with the Chimera embedded multi-block technique. A fully conservative, alternating direction implicit (ADI), approximately factored, partially fluxsplit algorithm was employed to perform the computation. Comparisons to some experimental wind tunnel data yielded good agreement for flow at zero incidence and angle of attack. The aim of this paper is to provide a methodology or computational tool for the numerical solution of complex external/internal flows.

  19. Independent measurement of the total active 8B solar neutrino flux using an array of 3He proportional counters at the Sudbury Neutrino Observatory.

    PubMed

    Aharmim, B; Ahmed, S N; Amsbaugh, J F; Anthony, A E; Banar, J; Barros, N; Beier, E W; Bellerive, A; Beltran, B; Bergevin, M; Biller, S D; Boudjemline, K; Boulay, M G; Bowles, T J; Browne, M C; Bullard, T V; Burritt, T H; Cai, B; Chan, Y D; Chauhan, D; Chen, M; Cleveland, B T; Cox-Mobrand, G A; Currat, C A; Dai, X; Deng, H; Detwiler, J; DiMarco, M; Doe, P J; Doucas, G; Drouin, P-L; Duba, C A; Duncan, F A; Dunford, M; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Ford, R J; Formaggio, J A; Fowler, M M; Gagnon, N; Germani, J V; Goldschmidt, A; Goon, J T M; Graham, K; Guillian, E; Habib, S; Hahn, R L; Hallin, A L; Hallman, E D; Hamian, A A; Harper, G C; Harvey, P J; Hazama, R; Heeger, K M; Heintzelman, W J; Heise, J; Helmer, R L; Henning, R; Hime, A; Howard, C; Howe, M A; Huang, M; Jagam, P; Jamieson, B; Jelley, N A; Keeter, K J; Klein, J R; Kormos, L L; Kos, M; Krüger, A; Kraus, C; Krauss, C B; Kutter, T; Kyba, C C M; Lange, R; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Loach, J C; MacLellan, R; Majerus, S; Mak, H B; Maneira, J; Martin, R; McBryde, K; McCauley, N; McDonald, A B; McGee, S; Mifflin, C; Miller, G G; Miller, M L; Monreal, B; Monroe, J; Morissette, B; Myers, A; Nickel, B G; Noble, A J; Oblath, N S; O'Keeffe, H M; Ollerhead, R W; Gann, G D Orebi; Oser, S M; Ott, R A; Peeters, S J M; Poon, A W P; Prior, G; Reitzner, S D; Rielage, K; Robertson, B C; Robertson, R G H; Rollin, E; Schwendener, M H; Secrest, J A; Seibert, S R; Simard, O; Simpson, J J; Sinclair, L; Skensved, P; Smith, M W E; Steiger, T D; Stonehill, L C; Tesić, G; Thornewell, P M; Tolich, N; Tsui, T; Tunnell, C D; Van Wechel, T; Van Berg, R; VanDevender, B A; Virtue, C J; Walker, T J; Wall, B L; Waller, D; Tseung, H Wan Chan; Wendland, J; West, N; Wilhelmy, J B; Wilkerson, J F; Wilson, J R; Wouters, J M; Wright, A; Yeh, M; Zhang, F; Zuber, K

    2008-09-12

    The Sudbury Neutrino Observatory (SNO) used an array of 3He proportional counters to measure the rate of neutral-current interactions in heavy water and precisely determined the total active (nu_x) 8B solar neutrino flux. This technique is independent of previous methods employed by SNO. The total flux is found to be 5.54_-0.31;+0.33(stat)-0.34+0.36(syst)x10(6) cm(-2) s(-1), in agreement with previous measurements and standard solar models. A global analysis of solar and reactor neutrino results yields Deltam2=7.59_-0.21;+0.19x10(-5) eV2 and theta=34.4_-1.2;+1.3 degrees. The uncertainty on the mixing angle has been reduced from SNO's previous results. PMID:18851271

  20. (2aR*,5S*,6aS*,8aS*,E)-Ethyl 5-hy­droxy-7,7,8a-trimethyl-8-oxo-2,2a,6,6a,7,8,8a,8b-octa­hydro-1H-penta­leno[1,6-bc]oxepine-4-carboxyl­ate

    PubMed Central

    Mehta, Goverdhan; Kumar, C. S. Ananda; Sen, Saikat

    2012-01-01

    The title compound, C17H24O5, featuring a 2-carbeth­oxy-3-oxepanone unit in its intra­molecularly O—H⋯O hydrogen-bonded enol form, was obtained via [(CF3CO2)2Rh]2-catal­ysed intra­molecular O—H bond insertion in the α-diazo-ω-hy­droxy-β-ketoester, ethyl 4-[(1S,3aS,6R,6aS)-6-hy­droxy-2,2,3a-trimethyl-3-oxo-octa­hydro­penta­len-1-yl]-2-diazo-3-oxobutano­ate. The seven-membered oxacyclic ring, thus constructed on a cis-fused diquinane platform, was found to adopt a distorted boat–sofa conformation. PMID:23476221

  1. 15 CFR 8a.305 - Preference in admission.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Preference in admission. 8a.305... on the Basis of Sex in Admission and Recruitment Prohibited § 8a.305 Preference in admission. A recipient to which §§ 8a.300 through 8a.310 apply shall not give preference to applicants for admission,...

  2. Knockdown of ATP8B1 expression leads to specific downregulation of the bile acid sensor FXR in HepG2 cells: effect of the FXR agonist GW4064.

    PubMed

    Martínez-Fernández, Pilar; Hierro, Loreto; Jara, Paloma; Alvarez, Luis

    2009-05-01

    Farnesoid X receptor (FXR) is a bile acid-sensing nuclear receptor that controls bile acid homeostasis. It has been suggested that downregulation of FXR contributes to the pathogenesis of an inherited disorder of bile secretion caused by mutations in ATP8B1. We have investigated the relationship between ATP8B1 knockdown and FXR downregulation in the human hepatoblastoma cell line HepG2. Transfection of HepG2 cells with ATP8B1 small interfering RNA (siRNA) duplexes led to a 60% reduction in the endogenous levels of ATP8B1 mRNA and protein and a concomitant decrease in FXR mRNA and protein content, as well as in FXR phosphorylation. This decrease was accompanied by a marked reduction in mRNA levels of a subset of FXR targets, such as bile salt export pump (ABCB11), small heterodimer partner, and uridine 5'-diphosphate-glucuronosyltransferase. ATP8B1 inhibition specifically targeted FXR since mRNA expression of other prominent nuclear receptors, such as pregnane X receptor and constitutive androstane receptor, or liver-enriched transcription factors, such as hepatocyte nuclear factor 1alpha (HNF-1alpha) and HNF-4alpha, was not altered. The expression of other key genes involved in bile acid synthesis, detoxification, and transport also remained unchanged upon ATP8B1 knockdown. Supporting the specificity of the effect, siRNA-mediated silencing of ABCB11, whose defect is associated with another inherited disorder of bile secretion, did not affect FXR expression. Treatment with the synthetic FXR agonist GW4064 was able to partially neutralize ATP8B1 siRNA-mediated FXR downregulation and fully counteract inhibition of FXR target genes. Collectively these findings indicate that ATP8B1 knockdown specifically downregulates FXR, and this action can be circumvented by treatment with FXR agonists.

  3. A functional N-terminal domain in C/EBPβ-LAP* is required for interacting with SWI/SNF and to repress Ric-8B gene transcription in osteoblasts.

    PubMed

    Aguilar, Rodrigo; Grandy, Rodrigo; Meza, Daniel; Sepulveda, Hugo; Pihan, Philippe; van Wijnen, Andre J; Lian, Jane B; Stein, Gary S; Stein, Janet L; Montecino, Martin

    2014-10-01

    The chromatin remodeling complex SWI/SNF and the transcription factor C/EBPβ play critical roles in osteoblastic cells as they jointly control transcription of a number of bone-related target genes. The largest C/EBPβ isoform, LAP*, possesses a short additional N-terminal domain that has been proposed to mediate the interaction of this factor with SWI/SNF in myeloid cells. Here we examine the requirement of a functional N-terminus in C/EBPβ-LAP* for binding SWI/SNF and for recruiting this complex to the Ric-8B gene to mediate transcriptional repression. We find that both C/EBPβ-LAP* and SWI/SNF simultaneously bind to the Ric-8B promoter in differentiating osteoblasts that repress Ric-8B expression. This decreased expression of Ric-8B is not accompanied by significant changes in histone acetylation at the Ric-8B gene promoter sequence. A single aminoacid change at the C/EBPβ-LAP* N-terminus (R3L) that inhibits C/EBPβ-LAP*-SWI/SNF interaction, also prevents SWI/SNF recruitment to the Ric-8B promoter as well as C/EBPβ-LAP*-dependent repression of the Ric-8B gene. Inducible expression of the C/EBPβ-LAP*R3L protein in stably transfected osteoblastic cells demonstrates that this mutant protein binds to C/EBPβ-LAP*-target promoters and competes with the endogenous C/EBPβ factor. Together our results indicate that a functional N-terminus in C/EBPβ-LAP* is required for interacting with SWI/SNF and for Ric-8B gene repression in osteoblasts.

  4. An Independent Measurement of the Total Active 8B Solar Neutrino Flux Using an Array of 3He Proportional Counters at the Sudbury Neutrino Observatory

    SciTech Connect

    SNO Colla

    2008-06-05

    The Sudbury Neutrino Observatory (SNO) used an array of {sup 3}He proportional counters to measure the rate of neutral-current interactions in heavy water and precisely determined the total active ({nu}{sub x}) {sup 8}B solar neutrino flux. This technique is independent of previous methods employed by SNO. The total flux is found to be 5.54{sub -0.31}{sup +0.33}(stat){sub -0.34}{sup +0.36}(syst) x 10{sup 6} cm{sup -2}s{sup -1}, in agreement with previous measurements and standard solar models. A global analysis of solar and reactor neutrino results yields {Delta}m{sup 2} = 7.94{sub -0.26}{sup +0.42} x 10{sup -5} eV{sup 2} and {theta} = 33.8{sub -1.3}{sup +1.4} degrees. The uncertainty on the mixing angle has been reduced from SNO's previous results.

  5. The lipid flippase heterodimer ATP8B1-CDC50A is essential for surface expression of the apical sodium-dependent bile acid transporter (SLC10A2/ASBT) in intestinal Caco-2 cells.

    PubMed

    van der Mark, Vincent A; de Waart, D Rudi; Ho-Mok, Kam S; Tabbers, Merit M; Voogt, Heleen W; Oude Elferink, Ronald P J; Knisely, A S; Paulusma, Coen C

    2014-12-01

    Deficiency of the phospholipid flippase ATPase, aminophospholipid transporter, class I, type 8B, member 1 (ATP8B1) causes progressive familial intrahepatic cholestasis type 1 (PFIC1) and benign recurrent intrahepatic cholestasis type 1 (BRIC1). Apart from cholestasis, many patients also suffer from diarrhea of yet unknown etiology. Here we have studied the hypothesis that intestinal ATP8B1 deficiency results in bile salt malabsorption as a possible cause of PFIC1/BRIC1 diarrhea. Bile salt transport was studied in ATP8B1-depleted intestinal Caco-2 cells. Apical membrane localization was studied by a biotinylation approach. Fecal bile salt and electrolyte contents were analyzed in stool samples of PFIC1 patients, of whom some had undergone biliary diversion or liver transplantation. Bile salt uptake by the apical sodium-dependent bile salt transporter solute carrier family 10 (sodium/bile acid cotransporter), member 2 (SLC10A2) was strongly impaired in ATP8B1-depleted Caco-2 cells. The reduced SLC10A2 activity coincided with strongly reduced apical membrane localization, which was caused by impaired apical membrane insertion of SLC10A2. Moreover, we show that endogenous ATP8B1 exists in a functional heterodimer with transmembrane protein 30A (CDC50A) in Caco-2 cells. Analyses of stool samples of post-transplant PFIC1 patients demonstrated that bile salt content was not changed, whereas sodium and chloride concentrations were elevated and potassium levels were decreased. The ATP8B1-CDC50A heterodimer is essential for the apical localization of SLC10A2 in Caco-2 cells. Diarrhea in PFIC1/BRIC1 patients has a secretory origin to which SLC10A2 deficiency may contribute. This results in elevated luminal bile salt concentrations and consequent enhanced electrolyte secretion and/or reduced electrolyte resorption.

  6. 48 CFR 6.204 - Section 8(a) competition.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Section 8(a) competition... ACQUISITION PLANNING COMPETITION REQUIREMENTS Full and Open Competition After Exclusion of Sources 6.204 Section 8(a) competition. (a) To fulfill statutory requirements relating to section 8(a) of the...

  7. 48 CFR 6.204 - Section 8(a) competition.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Section 8(a) competition... ACQUISITION PLANNING COMPETITION REQUIREMENTS Full and Open Competition After Exclusion of Sources 6.204 Section 8(a) competition. (a) To fulfill statutory requirements relating to section 8(a) of the...

  8. 48 CFR 6.204 - Section 8(a) competition.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Section 8(a) competition... ACQUISITION PLANNING COMPETITION REQUIREMENTS Full and Open Competition After Exclusion of Sources 6.204 Section 8(a) competition. (a) To fulfill statutory requirements relating to section 8(a) of the...

  9. 48 CFR 6.204 - Section 8(a) competition.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Section 8(a) competition... ACQUISITION PLANNING COMPETITION REQUIREMENTS Full and Open Competition After Exclusion of Sources 6.204 Section 8(a) competition. (a) To fulfill statutory requirements relating to section 8(a) of the...

  10. 48 CFR 6.204 - Section 8(a) competition.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Section 8(a) competition... ACQUISITION PLANNING COMPETITION REQUIREMENTS Full and Open Competition After Exclusion of Sources 6.204 Section 8(a) competition. (a) To fulfill statutory requirements relating to section 8(a) of the...

  11. Ric-8B interacts with G alpha olf and G gamma 13 and co-localizes with G alpha olf, G beta 1 and G gamma 13 in the cilia of olfactory sensory neurons.

    PubMed

    Kerr, Daniel S; Von Dannecker, Luiz Eduardo C; Davalos, Marcela; Michaloski, Jussara S; Malnic, Bettina

    2008-07-01

    Olfactory sensory neurons are able to detect odorants with high sensitivity and specificity. We have demonstrated that Ric-8B, a guanine nucleotide exchange factor (GEF), interacts with Galphaolf and enhances odorant receptor signaling. Here we show that Ric-8B also interacts with Ggamma13, a divergent member of the Ggamma subunit family which has been implicated in taste signal transduction, and is abundantly expressed in the cilia of olfactory sensory neurons. We show that Gbeta1 is the predominant Gbeta subunit expressed in the olfactory sensory neurons. Ric-8B and Gbeta1, like Galphaolf and Ggamma13, are enriched in the cilia of olfactory sensory neurons. We also show that Ric-8B interacts with Galphaolf in a nucleotide dependent manner, consistent with the role as a GEF. Our results constitute the first example of a GEF protein that interacts with two different olfactory G protein subunits and further implicate Ric-8B as a regulator of odorant signal transduction. PMID:18462949

  12. Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function.

    PubMed

    Kumar, Lalit; Chou, Janet; Yee, Christina S K; Borzutzky, Arturo; Vollmann, Elisabeth H; von Andrian, Ulrich H; Park, Shin-Young; Hollander, Georg; Manis, John P; Poliani, P Luigi; Geha, Raif S

    2014-05-01

    Lrrc8a is a ubiquitously expressed gene that encodes a leucine-rich repeat (LRR)-containing protein detected at higher levels on the surface of thymocytes than on other immune cells. We generated Lrrc8a(-/-) mice to investigate the role of LRRC8A in lymphocyte development and function. Lrrc8a(-/-) mice had increased prenatal and postnatal mortality, growth retardation, and multiple tissue abnormalities. Lrrc8a(-/-) mice displayed a modest block in B cell development but intact intrinsic B cell function. In contrast, both Lrrc8a(-/-) mice and Lrrc8a(-/-)→Rag2(-/-) bone marrow chimeras exhibited a severe cell-intrinsic block in early thymic development, with decreased proliferation and increased apoptosis of thymocytes, and impaired peripheral T cell function. Thymic epithelial cells expressed an LRRC8A ligand that was critical for double-negative to double-positive thymocyte differentiation and survival in vitro. LRRC8A constitutively associated with the GRB2-GAB2 complex and lymphocyte-specific protein tyrosine kinase (LCK) in thymocytes. LRRC8A ligation activated AKT via the LCK-ZAP-70-GAB2-PI3K pathway, and AKT phosphorylation was markedly reduced in the thymus of Lrrc8a(-/-) mice. These findings reveal an essential role for LRRC8A in T cell development, survival, and function.

  13. A case of Kallmann syndrome carrying a missense mutation in alternatively spliced exon 8A encoding the immunoglobulin-like domain IIIb of fibroblast growth factor receptor 1

    PubMed Central

    Miura, Kiyonori; Miura, Shoko; Yoshiura, Koh-ichiro; Seminara, Stephanie; Hamaguchi, Daisuke; Niikawa, Norio; Masuzaki, Hideaki

    2010-01-01

    Fibroblast growth factor receptor 1 (FGFR1) is one of the causative genes for Kallmann syndrome (KS), which is characterized by isolated hypogonadotropic hypogonadism with anosmia/hyposmia. The third immunoglobulin-like domain (D3) of FGFR1 has the isoforms FGFR1-IIIb and FGFR1-IIIc, which are generated by alternative splicing of exons 8A and 8B, respectively. To date, the only mutations to have been identified in D3 of FGFR1 are in exon 8B. We performed mutation analysis of FGFR1 in a 23-year-old female patient with KS and found a missense mutation (c.1072C>T) in exon 8A of FGFR1. The c.1072C>T mutation was not detected in her family members or in 220 normal Japanese and 100 Caucasian female controls. No mutation in other KS genes, KS 1, prokineticin-2, prokineticin receptor-2 and FGF-8 was detected in the affected patient or in her family members. Therefore, this is the first case of KS carrying a de novo missense mutation in FGFR1 exon 8A, suggesting that isoform FGFR1-IIIb, as well as isoform FGFR1-IIIc, plays a crucial role in the pathogenesis of KS. PMID:20139426

  14. The primary structure of component 8c-1, a subunit protein of intermediate filaments in wool keratin. Relationships with proteins from other intermediate filaments.

    PubMed Central

    Dowling, L M; Crewther, W G; Inglis, A S

    1986-01-01

    Component 8c-1, one of four highly homologous component-8 subunit proteins present in the microfibrils of wool, was isolated as its S-carboxymethyl derivative and its amino acid sequence was determined. Large peptides were isolated after cleaving the protein chemically or enzymically and the sequence of each was determined with an automatic Sequenator. The peptides were ordered by sequence overlaps and, in some instances, by homology with known sequences from other component-8 subunits. The C-terminal residues were identified by three procedures. Full details of the various procedures used have been deposited as Supplementary Publication SUP 50133 (4 pp.) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1986) 233, 5. The result showed that the protein comprises 412 residues and has an Mr, including the N-terminal acetyl group, of 48,300. The sequence of residues 98-200 of component 8c-1 was found to correspond to the partial or complete sequences of four homologous type I helical segments previously isolated from helical fragments recovered from chymotryptic digests of microfibrillar proteins of wool [Crewther & Dowling (1971) Appl. Polym. Symp. 18, 1-20; Crewther, Gough, Inglis & McKern (1978) Text. Res. J. 48, 160-162; Gough, Inglis & Crewther (1978) Biochem. J. 173, 385]. Considered in relation to amino acid sequences of other intermediate-filament proteins, the sequence is in accord with the view that keratin filament proteins are of two types [Hanukoglu & Fuchs (1983) Cell (Cambridge, Mass.) 33, 915-924]. Filament proteins from non-keratinous tissues, such as desmin, vimentin, neurofilament proteins and the glial fibrillary acidic protein, which form monocomponent filaments, constitute a third type. It is suggested that as a whole the proteins from intermediate filaments be classed as filamentins, the three types at present identified forming

  15. Crystal structures of two novel borate compounds MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8}

    SciTech Connect

    Li, H.K.; Cai, G.M.; Fan, J.J.; Jin, Z.P.; Zhou, T.T.; Chen, X.L.

    2013-06-15

    Two novel borate compounds MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8}, have been synthesized via solid-state reactions, and their crystal structures have been solved and refined from powder X-ray diffraction data. The compound MgInBO{sub 4}, which was obtained at 1190 °C, belongs to the warwickite family. It crystallizes in the Pnma space group (no. 62) with a=9.5443(1) Å, b=3.2771(1) Å, c=9.5228(1) Å, and Z=4. The fundamental building units are liner –Mg(In)O{sub 6}–[In(Mg)]{sub 2}O{sub 10}–Mg(In)O{sub 6}– chains and isolated BO{sub 3} triangles. The low-temperature phase, MgIn{sub 7/8}B{sub 7/8}O{sub 29/8}, whose crystal structure is solved ab initio by the charge-flipping method with standard chemical formula MgInBO{sub 4}, was prepared at 1080 °C. It crystallizes in the P12{sub 1}/n1 space group (no. 14) with a=17.0976(1) Å, b=3.2504(1) Å, c=5.3387(1) Å, β=96.0829(3)°, and Z=4. The structure of MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} contains [In(2)/Mg(2)]{sub 2}O{sub 10} groups, –MgO{sub 6}–InO{sub 6}– infinite ribbons and isolated BO{sub 3} triangles. The experiments and the differential thermal analysis (DTA) show the decompositions of MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} happen at about 1220 °C and 1180 °C, respectively. The comparative crystal chemistry from MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} to MgInBO{sub 4} has been discussed. Infrared spectra and UV–vis diffuse reflectance spectra of MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} were measured. - Graphical abstract: The structural transformation from the monoclinic MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} (P12{sub 1}/n1) to the orthorhombic MgInBO{sub 4} (Pnma). - Highlights: • Two novel borate compounds MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} have been synthesized via solid-state reactions for the first time. • The crystal structures of MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} have been solved and refined from powder X

  16. Ring Puckering Potentials of Three Fluorinated Cyclopentenes: C_5F_8, C_5HF_7, and C_5H_2F_6

    NASA Astrophysics Data System (ADS)

    Arsenault, E. A.; Long, B. E.; Pringle, Wallace C.; Choi, Yoon Jeong; Cooke, S. A.; Ocola, Esther J.; Laane, Jaan

    2015-06-01

    A systematic study on the ring puckering potentials of three fluorinated cyclopentenes has been performed using Fourier transform microwave spectroscopy in tandem with quantum chemical calculations. Spectra between 8 GHz and 16 GHz have been measured for octafluorocyclopentene, 1H-heptafluorocyclopentene, and 1H,2H-hexafluorocyclopentene, where the hydrogens sequentially replace the fluorines on the sp^2 hybridized carbons. Rotational constants and centrifugal distortion constants have been determined for the parent species and all 13C isotopologues. In regards to the ring puckering, double minimum potential, both cross state and intra-state transitions were observed for all molecules except the 1H,2H-hexafluorocyclopentene. Experimental Coriolis coupling constants and ΔE01 values will be presented and discussed. The ring puckering barrier heights for C_5F_8, C_5HF_7, and C_5H_2F_6, have been calculated to be 222 wn, 302 wn, and 367 wn, respectively.

  17. Expression pattern of NMDA receptors reveals antiepileptic potential of apigenin 8-C-glucoside and chlorogenic acid in pilocarpine induced epileptic mice.

    PubMed

    Aseervatham, G Smilin Bell; Suryakala, U; Doulethunisha; Sundaram, S; Bose, P Chandra; Sivasudha, T

    2016-08-01

    The present study was aimed to evaluate the effect of apigenin 8-C-glucoside (Vitexin) and chlorogenic acid on epileptic mice induced by pilocarpine and explored its possible mechanisms. Intraperitonial administration of pilocarpine (85mg/kg) induced seizure in mice was assessed by behavior observations, which is significantly (p>0.05) reduced by apigenin 8-C-glucoside (AP8CG) (10mg/kg) and chlorogenic acid (CA) (5mg/kg), similar to diazepam. Seizure was accompanied by an imbalance in the levels of Gamma-aminobutyric acid (GABA) and glutamate in the pilocarpine administered group. Moreover, convulsion along with reduced acetylcholinesterase, increased monoamine oxidase and oxidative stress was observed in epileptic mice brain. AP8CG and CA significantly restored back to normal levels even at lower doses. Further, increased lipid peroxidation and nitrite content was also significantly attenuated by AP8CG and CA. However, CA was found to be more effective when compared to AP8CG. In addition, the mRNA expression of N-methyl-d-aspartate receptor (NMDAR), mGluR1 and mGlu5 was significantly (P≤0.05) inhibited by AP8CG and CA in a lower dose. The mRNA expression of GRIK1 did not differ significantly in any of the group and showed a similar pattern of expression. Our result shows that AP8CG and CA selectively inhibit NMDAR, mGluR1 and mGlu5 expression. Modification in the provoked NMDAR calcium response coupled with neuronal death. Hence, these findings underline that the polyphenolics, AP8CG and CA have exerted antiepileptic and neuroprotective activity by suppressing glutamate receptors. PMID:27470339

  18. 38 CFR 8a.2 - Maximum amount of insurance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2011-07-01 2011-07-01 false Maximum amount of... MORTGAGE LIFE INSURANCE § 8a.2 Maximum amount of insurance. (a) Each eligible veteran is authorized up to a... amount of insurance in force as provided for in § 8a.4(a) the amount of VMLI thereafter available...

  19. 38 CFR 8a.2 - Maximum amount of insurance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false Maximum amount of... MORTGAGE LIFE INSURANCE § 8a.2 Maximum amount of insurance. (a) Each eligible veteran is authorized up to a... amount of insurance in force as provided for in § 8a.4(a) the amount of VMLI thereafter available...

  20. 15 CFR 8a.400 - Education programs or activities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Education programs or activities. 8a... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs...

  1. 15 CFR 8a.400 - Education programs or activities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Education programs or activities. 8a... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs...

  2. 15 CFR 8a.400 - Education programs or activities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Education programs or activities. 8a... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs...

  3. 15 CFR 8a.520 - Job classification and structure.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Job classification and structure. 8a... Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.520 Job classification and structure. A recipient shall not: (a) Classify a job as being for males or for females;...

  4. 15 CFR 8a.520 - Job classification and structure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Job classification and structure. 8a... Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.520 Job classification and structure. A recipient shall not: (a) Classify a job as being for males or for females;...

  5. 15 CFR 8a.520 - Job classification and structure.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Job classification and structure. 8a... Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.520 Job classification and structure. A recipient shall not: (a) Classify a job as being for males or for females;...

  6. 15 CFR 8a.520 - Job classification and structure.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Job classification and structure. 8a... Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.520 Job classification and structure. A recipient shall not: (a) Classify a job as being for males or for females;...

  7. 15 CFR 8a.520 - Job classification and structure.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Job classification and structure. 8a... Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.520 Job classification and structure. A recipient shall not: (a) Classify a job as being for males or for females;...

  8. 15 CFR 8a.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Education programs or activities. 8a... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs...

  9. Luteolin 8-C-β-fucopyranoside inhibits invasion and suppresses TPA-induced MMP-9 and IL-8 via ERK/AP-1 and ERK/NF-κB signaling in MCF-7 breast cancer cells.

    PubMed

    Park, Su-Ho; Kim, Jung-Hee; Lee, Dong-Hun; Kang, Jeong-Woo; Song, Hyuk-Hwan; Oh, Sei-Ryang; Yoon, Do-Young

    2013-11-01

    Matrix metalloproteinase 9 (MMP-9) and interleukin-8 (IL-8) play major roles in tumor progression and invasion of breast cancer cells. The present study was undertaken to investigate the inhibitory mechanism of cell invasion by luteolin 8-C-β-fucopyranoside (named as LU8C-FP), a C-glycosylflavone, in human breast cancer cells. We investigated whether LU8C-FP would inhibit MMP-9 activation and IL-8 expression in 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MCF-7 breast cancer cells. LU8C-FP suppressed TPA-induced MMP-9 and IL-8 secretion and mRNA expression via inhibition of the MAPK signaling pathway and down-regulation of nuclear AP-1 and NF-κB. TPA-induced phosphorylation of ERK 1/2 was suppressed by LU8C-FP, whereas JNK and p38 MAPK phosphorylation were unaffected. In addition, LU8C-FP blocked the ERK 1/2 pathways following expression of MMP-9 and IL-8. These results suggest LU8C-FP may function to suppress invasion of breast cancer cells through the ERK/AP-1 and ERK/NF-κB signaling cascades.

  10. SCN8A encephalopathy: Research progress and prospects.

    PubMed

    Meisler, Miriam H; Helman, Guy; Hammer, Michael F; Fureman, Brandy E; Gaillard, William D; Goldin, Alan L; Hirose, Shinichi; Ishii, Atsushi; Kroner, Barbara L; Lossin, Christoph; Mefford, Heather C; Parent, Jack M; Patel, Manoj; Schreiber, John; Stewart, Randall; Whittemore, Vicky; Wilcox, Karen; Wagnon, Jacy L; Pearl, Phillip L; Vanderver, Adeline; Scheffer, Ingrid E

    2016-07-01

    On April 21, 2015, the first SCN8A Encephalopathy Research Group convened in Washington, DC, to assess current research into clinical and pathogenic features of the disorder and prepare an agenda for future research collaborations. The group comprised clinical and basic scientists and representatives of patient advocacy groups. SCN8A encephalopathy is a rare disorder caused by de novo missense mutations of the sodium channel gene SCN8A, which encodes the neuronal sodium channel Nav 1.6. Since the initial description in 2012, approximately 140 affected individuals have been reported in publications or by SCN8A family groups. As a result, an understanding of the severe impact of SCN8A mutations is beginning to emerge. Defining a genetic epilepsy syndrome goes beyond identification of molecular etiology. Topics discussed at this meeting included (1) comparison between mutations of SCN8A and the SCN1A mutations in Dravet syndrome, (2) biophysical properties of the Nav 1.6 channel, (3) electrophysiologic effects of patient mutations on channel properties, (4) cell and animal models of SCN8A encephalopathy, (5) drug screening strategies, (6) the phenotypic spectrum of SCN8A encephalopathy, and (7) efforts to develop a bioregistry. A panel discussion of gaps in bioregistry, biobanking, and clinical outcomes data was followed by a planning session for improved integration of clinical and basic science research. Although SCN8A encephalopathy was identified only recently, there has been rapid progress in functional analysis and phenotypic classification. The focus is now shifting from identification of the underlying molecular cause to the development of strategies for drug screening and prioritized patient care. PMID:27270488

  11. DETAIL VIEW OF COMPUTER PANELS, ROOM 8A Cape Canaveral ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL VIEW OF COMPUTER PANELS, ROOM 8A - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

  12. 48 CFR 219.806 - Pricing the 8(a) contract.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., DEPARTMENT OF DEFENSE SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Contracting With the Small Business... officer shall notify the SBA prior to withdrawing a requirement from the 8(a) Program due to failure...

  13. 48 CFR 219.806 - Pricing the 8(a) contract.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., DEPARTMENT OF DEFENSE SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Contracting With the Small Business... officer shall notify the SBA prior to withdrawing a requirement from the 8(a) Program due to failure...

  14. 48 CFR 219.806 - Pricing the 8(a) contract.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., DEPARTMENT OF DEFENSE SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Contracting With the Small Business... officer shall notify the SBA prior to withdrawing a requirement from the 8(a) Program due to failure...

  15. "Dancing inside the ball": the structures and nonlinear optical properties of three Sc2S@C3v(8)-C82 isomers.

    PubMed

    Gao, Feng-Wei; Gao, Ying; Wang, Li-Jie; Xu, Hong-Liang; Sun, Shi-Ling; Su, Zhong-Min

    2015-10-01

    Recently, the crystal structures and electrochemical properties of the isomers (Sc2S "trapped" in C82) have been reported, in which the Sc2S is located inside the different positions of the C82 cage. In the present work, three isomers of endohedral metallofullerenes Sc2S@C3v(8)-C82 (A, B, and C) have been designed to explore the effect of the position of Sc2S on their interaction energies and nonlinear optical properties. Among three isomers, the Sc2S is located in different positions of the C82 cage: the angles of Sc-S-Sc in A, B, and C are 104.9, 114.8, and 115.7°, respectively. Furthermore, the analysis of natural bond orbital (NBO) charge indicates that the electron-transfer is from the Sc2S to the adjacent carbon atoms of the C82 cage. The interaction energy of B is the smallest among three isomers which is -226.2 kcal mol(-1). It was worth mentioning that their first hyperpolarizabilities (β tot) were studied, we found that their β tot values were related to the positions of Sc2S: C (2100) > B (1191) > A (947 au). We hope that the present work can provide a new strategy to promote the nonlinear optical properties of endohedral metallofullerenes by changing the positions of the encapsulated molecular. Graphical abstract Three isomers of endohedral metallofullerenes Sc2S@C3v(8)-C82 (A, B, and C) have been designed to explore the position effect of Sc2S on the interaction energies and nonlinear optical properties. Among three isomers, the Sc2S in B has the most stable position. Significantly, the first hyperpolarizability is related to the position of Sc2S inside the C82 cage, which provides a novel strategy to enhance the first hyperpolarizability by the Sc2S revolving inside the C82 cage.

  16. Nearly Perfect Spin Filter Based on a Wire of Half-Metallic (η5-C5H5)Ti (η8-C8H8)Ti Units

    NASA Astrophysics Data System (ADS)

    Zhu, Sicong; Fang, Jianming; Yao, Kailun; Wu, Yanqing

    2015-07-01

    Organic molecular devices show strong potential for future nanoelectronics applications, many of which require multifunction capability. Organometallic [(C5H5)Ti(C8H8)] n clusters are one of the few molecular material clusters that have been synthesized experimentally. Here we present a theoretical study of the spin-polarized transport on the clusters of [(C5H5)Ti(C8H8)] n by using nonequilibrium Green's functions combined with the density-functional theory. Results indicate that one-dimensional, organic (η5-C5H5)Ti (η8-C8H8)Ti wire has unique half-metallic properties with 100% spin polarization near the Fermi level in the ground state. We predict that finite-layer [(C5H5)Ti (C8H8)] n clusters coupled to gold electrodes exhibit a near-perfect spin-filter effect. Furthermore, due to the large spin coherence originated from its organic nature, these devices possess a spin-valve effect with large magnetoresistance. Our findings suggest that such a multifunctional molecular device is an excellent candidate for future molecule-based spintronic applications.

  17. Gα13 Stimulates the Tyrosine Phosphorylation of Ric-8A

    PubMed Central

    Yan, Mingda; Ha, Ji Hee

    2015-01-01

    The G12 family of heterotrimeric G proteins is defined by their α-subunits, Gα12 and Gα13. These α-subunits regulate cellular homeostasis, cell migration, and oncogenesis in a context-specific manner primarily through their interactions with distinct proteins partners that include diverse effector molecules and scaffold proteins. With a focus on identifying any other novel regulatory protein(s) that can directly interact with Gα13, we subjected Gα13 to tandem affinity purification-coupled mass spectrometric analysis. Our results from such analysis indicate that Gα13 potently interacts with mammalian Ric-8A. Our mass spectrometric analysis data also indicates that Ric-8A, which was tandem affinity purified along with Gα13, is phosphorylated at Ser-436, Thr-441, Thr-443 and Tyr-435. Using a serial deletion approach, we have defined that the C-terminus of Gα13 containing the guanine-ring interaction site is essential and sufficient for its interaction with Ric-8A. Evaluation of Gα13-specific signaling pathways in SKOV3 or HeyA8 ovarian cancer cell lines indicate that Ric-8A potentiates Gα13-mediated activation of RhoA, Cdc42, and the downstream p38MAPK. We also establish that the tyrosine phosphorylation of Ric-8A, thus far unidentified, is potently stimulated by Gα13. Our results also indicate that the stimulation of tyrosine-phosphorylation of Ric-8A by Gα13 is partially sensitive to inhibitors of Src-family of kinases, namely PP2 and SI. Furthermore, we demonstrate that Gα13 promotes the translocation of Ric-8A to plasma membrane and this translocation is attenuated by the Src-inhibitors, SI1 and PP2. Thus, our results demonstrate for the first time that Gα13 stimulates the tyrosine phosphorylation of Ric-8A and Gα13-mediated tyrosine-phosphorylation plays a critical role in the translocation of Ric-8A to plasma membrane. PMID:27096001

  18. Chimeric Antibody c.8B6 to O-Acetyl-GD2 Mediates the Same Efficient Anti-Neuroblastoma Effects as Therapeutic ch14.18 Antibody to GD2 without Antibody Induced Allodynia

    PubMed Central

    Cochonneau, Denis; Chaumette, Tanguy; Xiao, Wenhua; Diccianni, Mitchell B.; Barbet, Jacques; Yu, Alice L.; Paris, François; Sorkin, Linda S.; Birklé, Stéphane

    2014-01-01

    Background Anti-GD2 antibody is a proven therapy for GD2-postive neuroblastoma. Monoclonal antibodies against GD2, such as chimeric mAb ch14.18, have become benchmarks for neuroblastoma therapies. Pain, however, can limit immunotherapy with anti-GD2 therapeutic antibodies like ch14.18. This adverse effect is attributed to acute inflammation via complement activation on GD2-expressing nerves. Thus, new strategies are needed for the development of treatment intensification strategies to improve the outcome of these patients. Methodology/Principal Findings We established the mouse-human chimeric antibody c.8B6 specific to OAcGD2 in order to reduce potential immunogenicity in patients and to fill the need for a selective agent that can kill neuroblastoma cells without inducing adverse neurological side effects caused by anti-GD2 antibody immunotherapy. We further analyzed some of its functional properties compared with anti-GD2 ch14.18 therapeutic antibody. With the exception of allodynic activity, we found that antibody c.8B6 shares the same anti-neuroblastoma attributes as therapeutic ch14.18 anti-GD2 mAb when tested in cell-based assay and in vivo in an animal model. Conclusion/Significance The absence of OAcGD2 expression on nerve fibers and the lack of allodynic properties of c.8B6–which are believed to play a major role in mediating anti-GD2 mAb dose-limiting side effects–provide an important rationale for the clinical application of c.8B6 in patients with high-risk neuroblastoma. PMID:24520328

  19. 15 CFR 8a.530 - Marital or parental status.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... family unit. (b) Pregnancy. A recipient shall not discriminate against or exclude from employment any employee or applicant for employment on the basis of pregnancy, childbirth, false pregnancy, termination of pregnancy, or recovery therefrom. (c) Pregnancy as a temporary disability. Subject to § 8a.235(d),...

  20. 15 CFR 8a.445 - Marital or parental status.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) Pregnancy and related conditions. (1) A recipient shall not discriminate against any student, or exclude any... basis of such student's pregnancy, childbirth, false pregnancy, termination of pregnancy, or recovery... offered to non-pregnant students. (4) Subject to § 8a.235(d), a recipient shall treat...

  1. 8. A VIEW LOOKING NORTHEAST FROM SOUTHWEST OF THE BRIDGE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. A VIEW LOOKING NORTHEAST FROM SOUTHWEST OF THE BRIDGE, SHOWING THE INTRADOS OF THE ARCH, ITS ARRAS, ARCHED BELTING COURSE AND INCISED ELEMENTS IN THE SPANDRELS. THIS IMAGE ALSO SHOWS THE CONNECTION BETWEEN THE BRIDGE PROPER AND ITS ABUTMENT. - Vandalia Railroad Bridge, Spanning U.S. Route 40, Indianapolis, Marion County, IN

  2. 15 CFR 8a.415 - Access to course offerings.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.415 Access to course... or activity separately on the basis of sex, or require or refuse participation therein by any of...

  3. 15 CFR 8a.415 - Access to course offerings.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.415 Access to course... or activity separately on the basis of sex, or require or refuse participation therein by any of...

  4. 15 CFR 8a.415 - Access to course offerings.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.415 Access to course... or activity separately on the basis of sex, or require or refuse participation therein by any of...

  5. 15 CFR 8a.415 - Access to course offerings.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.415 Access to course... or activity separately on the basis of sex, or require or refuse participation therein by any of...

  6. 15 CFR 8a.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.400 Education programs or... basis of sex, be excluded from participation in, be denied the benefits of, or be subjected...

  7. 15 CFR 8a.120 - Transfers of property.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Introduction § 8a... in part with Federal financial assistance to a transferee that operates any education program or activity, and the Federal share of the fair market value of the property is not upon such sale or...

  8. 15 CFR 8a.530 - Marital or parental status.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... family unit. (b) Pregnancy. A recipient shall not discriminate against or exclude from employment any employee or applicant for employment on the basis of pregnancy, childbirth, false pregnancy, termination of pregnancy, or recovery therefrom. (c) Pregnancy as a temporary disability. Subject to § 8a.235(d),...

  9. 15 CFR 8a.530 - Marital or parental status.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... family unit. (b) Pregnancy. A recipient shall not discriminate against or exclude from employment any employee or applicant for employment on the basis of pregnancy, childbirth, false pregnancy, termination of pregnancy, or recovery therefrom. (c) Pregnancy as a temporary disability. Subject to § 8a.235(d),...

  10. 15 CFR 8a.445 - Marital or parental status.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) Pregnancy and related conditions. (1) A recipient shall not discriminate against any student, or exclude any... basis of such student's pregnancy, childbirth, false pregnancy, termination of pregnancy, or recovery... offered to non-pregnant students. (4) Subject to § 8a.235(d), a recipient shall treat...

  11. 15 CFR 8a.530 - Marital or parental status.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... family unit. (b) Pregnancy. A recipient shall not discriminate against or exclude from employment any employee or applicant for employment on the basis of pregnancy, childbirth, false pregnancy, termination of pregnancy, or recovery therefrom. (c) Pregnancy as a temporary disability. Subject to § 8a.235(d),...

  12. 15 CFR 8a.445 - Marital or parental status.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) Pregnancy and related conditions. (1) A recipient shall not discriminate against any student, or exclude any... basis of such student's pregnancy, childbirth, false pregnancy, termination of pregnancy, or recovery... offered to non-pregnant students. (4) Subject to § 8a.235(d), a recipient shall treat...

  13. 15 CFR 8a.445 - Marital or parental status.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) Pregnancy and related conditions. (1) A recipient shall not discriminate against any student, or exclude any... basis of such student's pregnancy, childbirth, false pregnancy, termination of pregnancy, or recovery... offered to non-pregnant students. (4) Subject to § 8a.235(d), a recipient shall treat...

  14. 15 CFR 8a.530 - Marital or parental status.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... family unit. (b) Pregnancy. A recipient shall not discriminate against or exclude from employment any employee or applicant for employment on the basis of pregnancy, childbirth, false pregnancy, termination of pregnancy, or recovery therefrom. (c) Pregnancy as a temporary disability. Subject to § 8a.235(d),...

  15. 15 CFR 8a.445 - Marital or parental status.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) Pregnancy and related conditions. (1) A recipient shall not discriminate against any student, or exclude any... basis of such student's pregnancy, childbirth, false pregnancy, termination of pregnancy, or recovery... offered to non-pregnant students. (4) Subject to § 8a.235(d), a recipient shall treat...

  16. 15 CFR 8a.435 - Employment assistance to students.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Employment assistance to students. 8a... to students. (a) Assistance by recipient in making available outside employment. A recipient that assists any agency, organization, or person in making employment available to any of its students:...

  17. 15 CFR 8a.435 - Employment assistance to students.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Employment assistance to students. 8a... to students. (a) Assistance by recipient in making available outside employment. A recipient that assists any agency, organization, or person in making employment available to any of its students:...

  18. 15 CFR 8a.435 - Employment assistance to students.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Employment assistance to students. 8a... to students. (a) Assistance by recipient in making available outside employment. A recipient that assists any agency, organization, or person in making employment available to any of its students:...

  19. 15 CFR 8a.435 - Employment assistance to students.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Employment assistance to students. 8a... to students. (a) Assistance by recipient in making available outside employment. A recipient that assists any agency, organization, or person in making employment available to any of its students:...

  20. 15 CFR 8a.435 - Employment assistance to students.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Employment assistance to students. 8a... to students. (a) Assistance by recipient in making available outside employment. A recipient that assists any agency, organization, or person in making employment available to any of its students:...

  1. In-depth understanding of the chemical properties of rarely explored carbide cluster metallofullerenes: a case study of Sc2 C2 @C3v (8)-C82 that reveals a general rule.

    PubMed

    Cai, Wenting; Chen, Muqing; Bao, Lipiao; Xie, Yunpeng; Akasaka, Takeshi; Lu, Xing

    2015-02-16

    The chemical properties of carbide-cluster metallofullerenes (CCMFs) remain largely unexplored, although several new members of CCMFs have been discovered recently. Herein, we report the reaction between Sc2 C2 @C3v (8)-C82 , which is viewed as a prototypical CCMF because of its high abundance, and 3-triphenylmethyl-5-oxazolidinone (1) to afford the corresponding pyrrolidino derivative Sc2 C2 @C3v (8)-C82 (CH2 )2 NTrt (2; Trt=triphenylmethyl). Single-crystal X-ray crystallography studies of 2 revealed that the reaction takes place at a [6,6]-bond junction, which is directly over the encapsulated C2 unit and is far from either of the two scandium atoms. On the basis of theoretical calculations and by considering previously reports, we have found that a hexagonal carbon ring on the cage of Sc2 C2 @C3v (8)-C82 is highly reactive toward different reagents due to the overlap of high p-orbital axis vector (POAV) angles and large LUMO coefficients. We propose that this highly concentrated area of reactivity is generated by the encapsulation of the Sc2 C2 cluster because this region is absent from the empty fullerene C3v (8)-C82 . Moreover, the absorption and electrochemical results confirm that derivative 2 is more stable than pristine Sc2 C2 @C3v (8)-C82 , thus illuminating its potential applications.

  2. ATOMIC AND MOLECULAR PHYSICS: Interference Angle on Quantum Rotational Energy Transfer in Na + Na2 (A1 Σ+u, v = 8 ~ b3п0u, v = 14) Molecular Collision System

    NASA Astrophysics Data System (ADS)

    Wang, Wei-Li; Miao, Gang; Li, Jian; Ma, Feng-Cai

    2009-12-01

    In order to study the collisional quantum interference (CQI) on rotational energy transfer in atom-diatom system, we have studied the relation of the integral interference angle and differential interference angle in Na + Na2 (A1 σ+u, v = 8 ~ b3п0u, v = 14) collision system. In this paper, based on the first-Born approximation of time-dependent perturbation theory and taking into accounts the anisotropic effect of Lennard-Jones interaction potentials, we present a theoretical model of collisional quantum interference in intramolecular rotational energy transfer, and a relationship between differential and integral interference angles.

  3. Crystal structure and Mössbauer studies of the isotypic Fe{sub 6}-cluster compounds RE{sub 15}[Fe{sub 8}C{sub 25}], RE=Dy, Ho

    SciTech Connect

    Davaasuren, Bambar; Dashjav, Enkhtsetseg; Kreiner, Guido; Borrmann, Horst; Weber, Sven-Ulf; Becker, Klaus-Dieter; Jochen Litterst, Fred; Kniep, Rüdiger

    2015-05-15

    The carboferrates RE{sub 15}[Fe{sub 8}C{sub 25}] (RE=Dy, Ho) were prepared from mixtures of the elements by arc-melting followed with subsequent annealing at 1373 K. The crystal structures were determined from single crystal X-ray diffraction data and revealed an isotypic relationship to Er{sub 15}[Fe{sub 8}C{sub 25}] (hP48, P321). The main feature of the crystal structure is given by Fe{sub 6} cluster units characterized by covalent Fe–Fe bonding interactions. {sup 57}Fe Mössbauer spectra of Dy{sub 15}[Fe{sub 8}C{sub 25}] were fitted by three subspectra with relative spectral weights of about 3:3:2 which is in general agreement with the crystal structure. Below 50 K, an onset of magnetic hyperfine fields at the three iron sites is observed which is supposed to be caused by dipolar fields arising from neighboring, slowly relaxing Dy magnetic moments. - Graphical abstract: Fe{sub 6}-cluster in the crystal structure of RE{sub 15}[Fe{sub 8}C{sub 25}], RE=Dy, Ho. - Highlights: • New carboferrates RE{sub 15}[Fe{sub 8}C{sub 25}] with RE=Dy, Ho have been synthesized. • The crystal structures were refined using single crystal X-ray data. • An orientational relationship between Fe{sub 6}-clusters and Fe in γ-Fe is outlined. • {sup 57}Fe Mössbauer spectra are in agreement with structural data from X-rays. • Magnetic hyperfine fields below 50 K are explained by dipolar fields from Dy atoms.

  4. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... syndrome, emotional or mental illness, and specific learning disabilities; (C) The term “physical or mental... disease, diabetes, mental retardation, emotional illness, drug addiction and alcoholism. (ii) Major life...) Handicapped person—(1) Handicapped person means any person who: (i) Has a physical or mental impairment...

  5. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... syndrome, emotional or mental illness, and specific learning disabilities; (C) The term “physical or mental... disease, diabetes, mental retardation, emotional illness, drug addiction and alcoholism. (ii) Major life...) Handicapped person—(1) Handicapped person means any person who: (i) Has a physical or mental impairment...

  6. 15 CFR 8b.8 - Notice.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... participants, beneficiaries, applicants and employees, including those with impaired vision or hearing, and... available to persons with impaired vision or hearing. (b) If a recipient publishes or uses...

  7. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... athletics shall provide to qualified handicapped students an equal opportunity for participation in these... of particular careers. (c) Social organizations. A recipient that provides significant assistance...

  8. 15 CFR 8b.17 - Existing facilities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... buildings, assignment of aides to beneficiaries, home visits, delivery of services at alternate accessible... accessible or the aid, benefit, or service must be made available at an alternative accessible site or sites... alternative, refer the qualified handicapped individual to other providers of those services that...

  9. 15 CFR 8b.17 - Existing facilities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... buildings, assignment of aides to beneficiaries, home visits, delivery of services at alternate accessible... accessible or the aid, benefit, or service must be made available at an alternative accessible site or sites... alternative, refer the qualified handicapped individual to other providers of those services that...

  10. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... more of the following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genito-urinary; hemic and lymphatic; skin... postsecondary institution, or a public system of higher education; or (ii) A local educational agency...

  11. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... more of the following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genito-urinary; hemic and lymphatic; skin... postsecondary institution, or a public system of higher education; or (ii) A local educational agency...

  12. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... interest in such property. (e) Federal financial assistance means any grant, loan, contract (other than a... Federal personnel; or (3) Real and personal property or any interest in or use of such property, including... syndrome, emotional or mental illness, and specific learning disabilities; (C) The term “physical or...

  13. 15 CFR 8b.13 - Employment criteria.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... sensory, manual, or speaking skills, the test results accurately to reflect the applicant's or employee's... applicant's or employee's impaired sensory, manual, or speaking skills (except where those skills are...

  14. 15 CFR 8b.12 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... physical or metal limitations of an otherwise qualified handicapped applicant or employee unless the...'s operation, including the composition and structure of the recipient's workforce; and (3)...

  15. 15 CFR 8b.14 - Preemployment inquiries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., That: (1) The recipient states clearly on any written questionnaire used for this purpose or makes clear orally, if no written questionnaire is used, that the information requested is intended for...

  16. 15 CFR 8b.22 - Academic adjustments.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... that impairs sensory, manual, or speaking skills as will best ensure that the results of the evaluation..., manual, or speaking skills (except where such skills are the factors that the test purports to measure..., or speaking skills. A recipient shall operate each program or activity to which this subpart...

  17. High hard magnetic properties and cellular structure of nanocomposite magnet Nd 4.5Fe 73.8B 18.5Cr 0.5Co 1.5Nb 1Cu 0.2

    NASA Astrophysics Data System (ADS)

    The, N. D.; Chau, N.; Vuong, N. V.; Quyen, N. H.

    2006-08-01

    The formation of special nanostructure, cellular structure, in Nd 4.5Fe 73.8B 18.5Cr 0.5Co 1.5Nb 1Cu 0.2 nanocomposite magnet has been observed by means of SEM for the first time. Ultrafine structure of cellules with thickness of 20-25 nm and length in range of 200-300 nm leads to high shape anisotropy of the materials. Therefore, high hard magnetic properties were obtained with ( BH) max up to 17.3 MG Oe in ribbons with very high remanence of 13.5 kG. The role of Cr and Co in the formation and refinement of cellular structure is proposed. Effect of heat treatment on hard magnetic properties is discussed in detail.

  18. 13 CFR 124.204 - How does SBA process applications for 8(a) BD program admission?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... for 8(a) BD program admission? 124.204 Section 124.204 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Applying to the 8(a) Bd Program § 124.204 How does SBA process applications for 8(a) BD...

  19. Biological assessment: possible impacts of exploratory drilling in sections 8B and 18H, Naval Petroleum Reserve No. 2, Kern County, California on the endangered San Joaquin kit fox, blunt-nosed leopard lizard, and other sensitive species

    SciTech Connect

    O'Farrell, T.P.; Sauls, M.L.

    1982-07-01

    The U.S. Department of Energy proposes to drill exploratory wells on two sections, 8B and 18H, within Naval Petroleum Reserve No. 2 in western Kern County, California. The proposed sites are thought to provide habitat for the endangered San Joaquin kit fox and blunt-nosed leopard lizard, as well as two sensitive species: the giant kangaroo rat and San Joaquin antelope ground squirrel. The objective was to assess the possible impacts of the exploratory drilling on these species and their essential habitats. Although 23 potential San Joaquin kit fox den sites were found during surveys of a total of 512 ha (1280 acres) surrounding both well sites, no burrows were closer than 30 m from proposed disturbance, and most were over 200 m away. Two blunt-nosed leopard lizards were observed on private land within 8B, one was observed on private land in 18H, and two were seen on DOE portions of 18H. No evidence of blunt-nosed leopard lizards was gathered in the immediate vicinity of either proposed well site. Although 5 ha of habitat will be disturbed, there is no evidence to indicate any of the species has burrows on-site that will be lost during land clearing. Loss of habitat will be mitigated during the cleanup and restoration phases when disturbed areas will be revegetated. Increased traffic, human activities, noise and ground vibration levels, as well as illumination throughout the night, may disturb the fauna. However, these species have adapted to intensive human disturbances on Elk Hills without obvious negative effects. The short duration of the project should allow any displaced animals to return to the sites after drilling ceases.

  20. Role of IncP-1β plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses.

    PubMed

    Król, J E; Penrod, J T; McCaslin, H; Rogers, L M; Yano, H; Stancik, A D; Dejonghe, W; Brown, C J; Parales, R E; Wuertz, S; Top, E M

    2012-02-01

    Broad-host-range catabolic plasmids play an important role in bacterial degradation of man-made compounds. To gain insight into the role of these plasmids in chloroaniline degradation, we determined the first complete nucleotide sequences of an IncP-1 chloroaniline degradation plasmid, pWDL7::rfp and its close relative pNB8c, as well as the expression pattern, function, and bioaugmentation potential of the putative 3-chloroaniline (3-CA) oxidation genes. Based on phylogenetic analysis of backbone proteins, both plasmids are members of a distinct clade within the IncP-1β subgroup. The plasmids are almost identical, but whereas pWDL7::rfp carries a duplicate inverted catabolic transposon, Tn6063, containing a putative 3-CA oxidation gene cluster, dcaQTA1A2BR, pNB8c contains only a single copy of the transposon. No genes for an aromatic ring cleavage pathway were detected on either plasmid, suggesting that only the upper 3-CA degradation pathway was present. The dcaA1A2B gene products expressed from a high-copy-number vector were shown to convert 3-CA to 4-chlorocatechol in Escherichia coli. Slight differences in the dca promoter region between the plasmids and lack of induction of transcription of the pNB8c dca genes by 3-CA may explain previous findings that pNB8C does not confer 3-CA transformation. Bioaugmentation of activated sludge with pWDL7::rfp accelerated removal of 3-CA, but only in the presence of an additional carbon source. Successful bioaugmentation requires complementation of the upper pathway genes with chlorocatechol cleavage genes in indigenous bacteria. The genome sequences of these plasmids thus help explain the molecular basis of their catabolic activities.

  1. Formation of metallaboranes from the group IV transition metals and pentaborane(9): crystal and molecular structure of [(Cp(2)Zr)(2)B(5)H(8)][B(11)H(14)].

    PubMed

    Thomas, Rhodri Ll; Rath, Nigam P; Barton, Lawrence

    2002-01-14

    The reactions between [(C(5)H(5))(2)MCl(2)] (where M = Ti, Zr, Hf) and Li[B(5)H(8)] in a variety of solvents have been investigated. In the case of Zr, a pale orange solid, mu-(Cp(2)ClZr)B(5)H(8) (1), is formed in 70% yield. Compound 1 exists as a B(5)H(9) cage with a Cp(2)ClZr moiety replacing a bridging H atom. The variable temperature NMR spectra of 1 reveal two fluxional processes, one (DeltaG(++) = 54 kJ mol(-1)) which renders a plane of symmetry in the molecule and a higher temperature one (DeltaG(++) = 48 kJ mol(-1)) which renders all the basal B atoms equivalent. Dynamic processes are suggested to account for these observations. Passage of a CH(2)Cl(2) solution of 1 through silica gel affords 2, [(Cp(2)Zr)(2)B(5)H(8)][B(11)H(14)], a yellow, air-stable, crystalline solid, in 14% yield. The cation in 2, [(Cp(2)Zr)(2)B(5)H(8)](+), consists of a distorted spiro[2.2]pentane-like B(5) moiety comprising two B(3) triangles sharing a naked boron vertex. The two triangles are twisted 73 degrees with respect to each other, and the two [Cp(2)Zr] groups bond in a trihapto arrangement to the two opposite B-B-B edges. Each exterior B-Zr edge is H-bridged, and the B atoms possess terminal hydrogens. Reactions of Cp(2)HfCl(2) with Li[B(5)H(8)] lead to the formation of the analogue of 2, [(Cp(2)Hf)(2)B(5)H(8)][B(11)H(14)] (3). The precursor to 3, that is, the Hf analogue of 1, is not observed. Reaction between Li[B(5)H(8)] and Cp(2)TiCl(2) afforded no identifiable products, but reaction with CpTiCl(3) resulted in cage coupling and the formation of B(10)H(14). PMID:11782145

  2. 13 CFR 124.501 - What general provisions apply to the award of 8(a) contracts?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Contractual Assistance § 124.501 What general provisions apply to the award of 8(a) contracts? (a... awards won through competition with other Participants. (c) Admission into the 8(a) BD program does...

  3. 13 CFR 124.509 - What are non-8(a) business activity targets?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... competitive marketplace after graduating from the 8(a) BD program, Participants must make maximum efforts to... reasonable marketing strategy, to attain the targeted dollar levels of non-8(a) revenue established in its... source or competitive 8(a) contracts). These targets are called non-8(a) business activity targets...

  4. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genitourinary; hemic and lymphatic; skin; and endocrine... that term is defined for purposes of employment in 29 CFR 1613.702(f), which is made applicable to...

  5. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genitourinary; hemic and lymphatic; skin; and endocrine... that term is defined for purposes of employment in 29 CFR 1613.702(f), which is made applicable to...

  6. 15 CFR 8c.40 - Employment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... discrimination in employment under any program or activity conducted by the agency. The definitions, requirements, and procedures of section 501 of the Rehabilitation Act of 1973 (29 U.S.C. 791), as established by the Equal Employment Opportunity Commission in 29 CFR Part 1613, shall apply to employment in...

  7. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genitourinary; hemic and lymphatic; skin; and endocrine... that term is defined for purposes of employment in 29 CFR 1613.702(f), which is made applicable to...

  8. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... impairments, cerebral palsy, epilepsy, muscular dystrophy, multiple sclerosis, cancer, heart disease, diabetes... that term is defined for purposes of employment in 29 CFR 1613.702(f), which is made applicable to...

  9. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... impairments, cerebral palsy, epilepsy, muscular dystrophy, multiple sclerosis, cancer, heart disease, diabetes... that term is defined for purposes of employment in 29 CFR 1613.702(f), which is made applicable to...

  10. 8-C N-ethyl-2-pyrrolidinone substituted flavan-3-ols as the marker compounds of Chinese dark teas formed in the post-fermentation process provide significant antioxidative activity.

    PubMed

    Wang, Weinan; Zhang, Liang; Wang, Shu; Shi, Shepo; Jiang, Yong; Li, Ning; Tu, Pengfei

    2014-01-01

    Phytochemical investigation of the aqueous extract of pu-erh tea afforded eight novel 8-C N-ethyl-2-pyrrolidinone substituted flavan-3-ols (puerins I-VIII) by (1)H, (13)C, two-dimensional nuclear magnetic resonance (NMR) and high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS) analysis. Comparative chemical analysis of green tea, black tea and Chinese dark teas confirmed that these compounds were the marker compounds of Chinese dark teas. Furthermore, fungal fermentation was indispensable for the biosynthesis of these novel compounds. Through single fungal fermentation, it was proved that catechins and theanine were the precursors of puerins I-VIII. HPLC-DAD-ESI/MS analysis elucidated the biosynthetic pathway for puerins I-VIII. Puerins I-IV have potential protective effects for the human micro-vascular endothelial cells (HMEC) injury induced by hydrogen dioxide compared to other tea polyphenols. 8-C N-ethyl-2-pyrrolidinone substituted flavan-3-ols could be used in the quality control and authentication of Chinese dark teas. PMID:24444972

  11. Evaluation of Cavity Collapse and Surface Crater Formation at the Norbo Underground Nuclear Test in U8c, Nevada Nuclear Security Site, and the Impact on Stability of the Ground Surface

    SciTech Connect

    Pawloski, G A

    2012-06-18

    Lawrence Livermore National Laboratory (LLNL) Containment Program performed a review of nuclear test-related data for the Norbo underground nuclear test in U8c to assist in evaluating this legacy site as a test bed for application technologies for use in On-Site Inspections (OSI) under the Comprehensive Nuclear Test Ban Treaty. This request is similar to one made for the Salut site in U8c (Pawloski, 2012b). Review of the Norbo site is complicated because the test first exhibited subsurface collapse, which was not unusual, but it then collapsed to the surface over one year later, which was unusual. Of particular interest is the stability of the ground surface above the Norbo detonation point. Proposed methods for on-site verification include radiological signatures, artifacts from nuclear testing activities, and imaging to identify alteration to the subsurface hydrogeology due to the nuclear detonation. Aviva Sussman from the Los Alamos National Laboratory (LANL) has also proposed work at this site. Both proposals require physical access at or near the ground surface of specific underground nuclear test locations at the Nevada Nuclear Security Site (NNSS), formerly the Nevada Test Site (NTS), and focus on possible activities such as visual observation, multispectral measurements, and shallow and deep geophysical surveys.

  12. 17 CFR Appendix A to Part 3 - Interpretative Statement With Respect to Section 8a(2)(C) and (E) and Section 8a(3)(J) and (M) of...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... exchange imposed a relatively minor sanction. 2 Commission rules referred to herein are found at 17 CFR Ch...”), including settled disciplinary actions. 5 Commission rules referred to in this letter are found at 17 CFR Ch... Respect to Section 8a(2)(C) and (E) and Section 8a(3)(J) and (M) of the Commodity Exchange Act A...

  13. 48 CFR 619.803-71 - Simplified procedures for 8(a) acquisitions under MOUs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Registration database (http://www.ccr.gov) to establish that the selected 8(a) firm is a current program... the price with the selected 8(a) contractor. (d) The contracting officer shall issue the...

  14. 48 CFR 252.219-7009 - Section 8(a) direct award.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Contracting Officer, simultaneous with its notification to the SBA (as required by SBA's 8(a) regulations at 13 CFR 124.308), when the owner or owners upon whom 8(a) eligibility is based plan to...

  15. 15 CFR 8a.135 - Designation of responsible employee and adoption of grievance procedures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Designation of responsible employee and adoption of grievance procedures. 8a.135 Section 8a.135 Commerce and Foreign Trade Office of the... FEDERAL FINANCIAL ASSISTANCE Introduction § 8a.135 Designation of responsible employee and adoption...

  16. 15 CFR 8a.425 - Counseling and use of appraisal and counseling materials.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Counseling and use of appraisal and counseling materials. 8a.425 Section 8a.425 Commerce and Foreign Trade Office of the Secretary of Commerce... Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.425 Counseling and use...

  17. 15 CFR 8a.425 - Counseling and use of appraisal and counseling materials.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Counseling and use of appraisal and counseling materials. 8a.425 Section 8a.425 Commerce and Foreign Trade Office of the Secretary of Commerce... Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.425 Counseling and use...

  18. 15 CFR 8a.425 - Counseling and use of appraisal and counseling materials.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Counseling and use of appraisal and counseling materials. 8a.425 Section 8a.425 Commerce and Foreign Trade Office of the Secretary of Commerce... Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.425 Counseling and use...

  19. 15 CFR 8a.425 - Counseling and use of appraisal and counseling materials.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Counseling and use of appraisal and counseling materials. 8a.425 Section 8a.425 Commerce and Foreign Trade Office of the Secretary of Commerce... Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.425 Counseling and use...

  20. 15 CFR 8a.425 - Counseling and use of appraisal and counseling materials.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Counseling and use of appraisal and counseling materials. 8a.425 Section 8a.425 Commerce and Foreign Trade Office of the Secretary of Commerce... Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.425 Counseling and use...

  1. 48 CFR 1052.219-72 - Section 8(a) direct awards.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Section 8(a) direct awards. As prescribed in 1019.811-3(f), insert the following clause: 8(a) Business Development Program Awards (JUN 2003) (a) This purchase/delivery/task order or contract is issued by the... under the 8(a) Business Development program. The cognizant SBA district office is: (b) The...

  2. 13 CFR 124.3 - What definitions are important in the 8(a) BD program?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business... terms. Eligibility Requirements for Participation in the 8(a) Business Development Program ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What definitions are important...

  3. 13 CFR 124.507 - What procedures apply to competitive 8(a) procurements?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What procedures apply to competitive 8(a) procurements? 124.507 Section 124.507 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a)...

  4. 13 CFR 124.508 - How is an 8(a) contract executed?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false How is an 8(a) contract executed? 124.508 Section 124.508 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development...

  5. 13 CFR 124.1 - What is the purpose of the 8(a) Business Development program?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) Business Development program? 124.1 Section 124.1 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Provisions of General Applicability § 124.1 What is the purpose of the 8(a) Business...

  6. 13 CFR 124.508 - How is an 8(a) contract executed?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false How is an 8(a) contract executed? 124.508 Section 124.508 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development...

  7. 13 CFR 124.501 - What general provisions apply to the award of 8(a) contracts?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What general provisions apply to the award of 8(a) contracts? 124.501 Section 124.501 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a)...

  8. 13 CFR 124.501 - What general provisions apply to the award of 8(a) contracts?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What general provisions apply to the award of 8(a) contracts? 124.501 Section 124.501 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a)...

  9. 13 CFR 124.511 - How is fair market price determined for an 8(a) contract?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false How is fair market price determined for an 8(a) contract? 124.511 Section 124.511 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a)...

  10. 13 CFR 124.1 - What is the purpose of the 8(a) Business Development program?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) Business Development program? 124.1 Section 124.1 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Provisions of General Applicability § 124.1 What is the purpose of the 8(a) Business...

  11. 48 CFR 52.219-18 - Notification of Competition Limited to Eligible 8(a) Concerns.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... made to the Small Business Administration, which will subcontract performance to the successful 8(a... Eligible 8(a) Concerns (JUN 2003) (a) Offers are solicited only from small business concerns expressly certified by the Small Business Administration (SBA) for participation in the SBA's 8(a) Program and...

  12. 13 CFR 124.507 - What procedures apply to competitive 8(a) procurements?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What procedures apply to competitive 8(a) procurements? 124.507 Section 124.507 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a)...

  13. 40 CFR Appendix A to Subpart Bb of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... performance specifications. 63.8(a)(3) No . 63.8(a)(4) Yes. 63.8(b) Conduct of Monitoring Yes. 63.8(c)(1... or CMS performance specifications. 63.8(d) Quality Control Yes. 63.8(e) CMS Performance Evaluation No...)(6) No Subpart BB does not require CMS performance specifications. 63.10(c)(7) and (8) Yes....

  14. Post-transcriptional regulation of wnt8a is essential to zebrafish axis development.

    PubMed

    Wylie, Annika D; Fleming, Jo-Ann G W; Whitener, Amy E; Lekven, Arne C

    2014-02-01

    wnt8a Is essential for normal patterning during vertebrate embryonic development, and either gain or loss-of-function gene dysregulation results in severe axis malformations. The zebrafish wnt8a locus is structured such that transcripts may possess two regulatory 3' untranslated regions (UTRs), raising the possibility of post-transcriptional regulation as an important mode of wnt8a signaling control. To determine whether both UTRs contribute to post-transcriptional wnt8a gene regulation, each UTR (UTR1 and UTR2) was tested in transient and transgenic reporter assays. Both UTRs suppress EGFP reporter expression in cis, with UTR2 exhibiting a more pronounced effect. UTR2 contains a 6 base sequence necessary for UTR2 regulatory function that is complementary to the seed of the microRNA, miR-430. A target protector morpholino that overlaps the seed complement stabilizes both reporter mRNAs and wnt8a mRNAs, and produces phenotypic abnormalities consistent with wnt8a gain-of-function. In rescue assays, specific functions can be attributed to each of the two wnt8a proteins encoded by the locus. An interplay of wnt8a.1 and wnt8a.2 regulates neural and mesodermal patterning and morphogenesis as well as patterning between brain subdivisions. Thus, post-transcriptional control of wnt8a is essential to fine tune the balance of the signaling outputs of the complex wnt8a locus.

  15. Kasatkinite, Ba2Ca8B5Si8O32(OH)3 · 6H2O6, a new mineral from the Bazhenovskoe deposit, the Central Urals, Russia

    NASA Astrophysics Data System (ADS)

    Pekov, I. V.; Chukanov, N. V.; Filinchuk, Ya. E.; Zadov, A. E.; Kononkova, N. N.; Epanchintsev, S. G.; Kaden, P.; Kutzer, A.; Göttlicher, J.

    2013-12-01

    A new mineral, kasatkinite, Ba2Ca8B5Si8O32(OH)3 · 6H2O, has been found at the Bazhenovskoe chrysotile asbestos deposit, the Central Urals, Russia in the cavities in rhodingite as a member of two assemblages: (l) on prehnite, with pectolite, calcite, and clinochlore; and (2) on grossular, with diopside and pectolite. Kasatkinite occurs as spherulites or bunches up to 3 mm in size, occasionally combined into crusts. Its individuals are acicular to hair-like, typically split, with a polygonal cross section, up to 0.5 mm (rarely, to 6 mm) in length and to 20 μm in thickness. They consist of numerous misoriented needle-shaped subindividuals up to several dozen μm long and no more than 1 μm thick. Kasatkinite individuals are transparent and colorless; its aggregates are snow white. The luster is vitreous or silky. No cleavage was observed; the fracture is uneven or splintery for aggregates. Individuals are flexible and elastic. The Mohs' hardness is 4-4.5. D meas = 2.95(5), D calc = 2.89 g/cm3. Kasatkinite is optically biaxial (+), α = 1.600(5), β = 1.603(2), γ = 1.626(2), 2 V meas = 30(20)°, 2 V calc = 40°. The IR spectrum is given. The 11B MAS NMR spectrum shows the presence of BO4 in the absence of BO3 groups. The chemical composition of kasatkinite (wt %; electron microprobe, H2O by gas chromatography) is as follows: 0.23 Na2O, 0.57 K2O, 28.94 CaO, 16.79 BaO, 11.57 B2O3, 0.28 Al2O3, 31.63 SiO2, 0.05 F, 9.05 H2O, -0.02 -O=F2; the total is 99.09. The empirical formula (calculated on the basis of O + F = 41 apfu, taking into account the TGA data) is: Na0.11K0.18Ba1.66Ca7.84B5.05Al0.08Si8.00O31.80(OH)3.06F0.04 · 6.10H2O. Kasatkinite is monoclinic, space group P21/ c, P2/ c, or Pc; the unit-cell dimensions are a = 5.745(3), b = 7.238(2), c = 20.79 (1) Å, β = 90.82(5)°, V = 864(1) Å3, Z = 1. The strongest reflections ( d Å- I[ hkl]) in the X-ray powder diffractions pattern are: 5.89-24[012], 3.48-2.1[006], 3.36-24[114]; 3.009-100[, 121, ], 2

  16. Rbm8a Haploinsufficiency Disrupts Embryonic Cortical Development Resulting in Microcephaly

    PubMed Central

    Mao, Hanqian; Pilaz, Louis-Jan; McMahon, John J.; Golzio, Christelle; Wu, Danwei; Shi, Lei; Katsanis, Nicholas

    2015-01-01

    The cerebral cortex is built during embryonic neurogenesis, a period when excitatory neurons are generated from progenitors. Defects in neurogenesis can cause acute neurodevelopmental disorders, such as microcephaly (reduced brain size). Altered dosage of the 1q21.1 locus has been implicated in the etiology of neurodevelopmental phenotypes; however, the role of 1q21.1 genes in neurogenesis has remained elusive. Here, we show that haploinsufficiency for Rbm8a, an exon junction complex (EJC) component within 1q21.1, causes severe microcephaly and defective neurogenesis in the mouse. At the onset of neurogenesis, Rbm8a regulates radial glia proliferation and prevents premature neuronal differentiation. Reduced Rbm8a levels result in subsequent apoptosis of neurons, and to a lesser extent, radial glia. Hence, compared to control, Rbm8a-haploinsufficient brains have fewer progenitors and neurons, resulting in defective cortical lamination. To determine whether reciprocal dosage change of Rbm8a alters embryonic neurogenesis, we overexpressed human RBM8A in two animal models. Using in utero electroporation of mouse neocortices as well as zebrafish models, we find RBM8A overexpression does not significantly perturb progenitor number or head size. Our findings demonstrate that Rbm8a is an essential neurogenesis regulator, and add to a growing literature highlighting roles for EJC components in cortical development and neurodevelopmental pathology. Our results indicate that disruption of RBM8A may contribute to neurodevelopmental phenotypes associated with proximal 1q21.1 microdeletions. PMID:25948253

  17. 17 CFR 239.14 - Form N-2 for closed end management investment companies registered on Form N-8A.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and at www... closed end management investment companies registered under the Investment Company Act of 1940 on form N... companies pursuant to section 8(b) of the Investment Company Act of 1940 (§ 274.11a-1 of this chapter)....

  18. 17 CFR 239.14 - Form N-2 for closed end management investment companies registered on Form N-8A.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and at www... closed end management investment companies registered under the Investment Company Act of 1940 on form N... companies pursuant to section 8(b) of the Investment Company Act of 1940 (§ 274.11a-1 of this chapter)....

  19. 17 CFR 239.14 - Form N-2 for closed end management investment companies registered on Form N-8A.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and on GPO... closed end management investment companies registered under the Investment Company Act of 1940 on form N... companies pursuant to section 8(b) of the Investment Company Act of 1940 (§ 274.11a-1 of this chapter)....

  20. 17 CFR 239.14 - Form N-2 for closed end management investment companies registered on Form N-8A.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and at www... closed end management investment companies registered under the Investment Company Act of 1940 on form N... companies pursuant to section 8(b) of the Investment Company Act of 1940 (§ 274.11a-1 of this chapter)....

  1. 17 CFR 239.14 - Form N-2 for closed end management investment companies registered on Form N-8A.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... of CFR Sections Affected, which appears in the Finding Aids section of the printed volume and at www... closed end management investment companies registered under the Investment Company Act of 1940 on form N... companies pursuant to section 8(b) of the Investment Company Act of 1940 (§ 274.11a-1 of this chapter)....

  2. 19 CFR 10.8a - Imported articles exported and reimported.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Imported articles exported and reimported. 10.8a...; DEPARTMENT OF THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. General Provisions Articles Exported and Returned § 10.8a Imported articles exported and reimported. (a) In addition...

  3. 19 CFR 10.8a - Imported articles exported and reimported.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 1 2012-04-01 2012-04-01 false Imported articles exported and reimported. 10.8a...; DEPARTMENT OF THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. General Provisions Articles Exported and Returned § 10.8a Imported articles exported and reimported. (a) In addition...

  4. 19 CFR 10.8a - Imported articles exported and reimported.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 1 2011-04-01 2011-04-01 false Imported articles exported and reimported. 10.8a...; DEPARTMENT OF THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. General Provisions Articles Exported and Returned § 10.8a Imported articles exported and reimported. (a) In addition...

  5. 19 CFR 10.8a - Imported articles exported and reimported.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 1 2014-04-01 2014-04-01 false Imported articles exported and reimported. 10.8a...; DEPARTMENT OF THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. General Provisions Articles Exported and Returned § 10.8a Imported articles exported and reimported. (a) In addition...

  6. 13 CFR 124.204 - How does SBA process applications for 8(a) BD program admission?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Development Applying to the 8(a) Bd Program § 124.204 How does SBA process applications for 8(a) BD program... processing. The appropriate field office will advise each program applicant within 15 days after the receipt... rights. (f) If the AA/BD approves the application, the date of the approval letter is the date of...

  7. 48 CFR 2452.219-72 - Section 8(a) direct awards (Deviation).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... URBAN DEVELOPMENT CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 2452.219-72 Section 8(a) direct awards (Deviation). As prescribed in 2419.811-3(f), insert the... the Department of Housing and Urban Development (HUD) and the 8(a) Contractor pursuant to...

  8. 75 FR 60263 - Federal Acquisition Regulation; Offering a Construction Requirement-8(a) Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-29

    ... Federal Acquisition Regulation; Offering a Construction Requirement--8(a) Program AGENCIES: Department of... Regulations Council (Councils) are issuing a final rule amending the Federal Acquisition Regulation (FAR) to revise FAR subpart 19.8, Contracting with the Small Business Administration (The 8(a) Program),...

  9. 13 CFR 124.509 - What are non-8(a) business activity targets?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... activity targets? 124.509 Section 124.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a... Contractual Assistance § 124.509 What are non-8(a) business activity targets? (a) General. (1) To ensure that...) business activity targets during transitional stage—(1) General. During the transitional stage of the...

  10. 13 CFR 124.509 - What are non-8(a) business activity targets?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... activity targets? 124.509 Section 124.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a... Contractual Assistance § 124.509 What are non-8(a) business activity targets? (a) General. (1) To ensure that...) business activity targets during transitional stage—(1) General. During the transitional stage of the...

  11. 13 CFR 124.509 - What are non-8(a) business activity targets?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... activity targets? 124.509 Section 124.509 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a... Contractual Assistance § 124.509 What are non-8(a) business activity targets? (a) General. (1) To ensure that...) business activity targets during transitional stage—(1) General. During the transitional stage of the...

  12. 17 CFR 274.10 - Form N-8A, for notification of registration.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... filed with the Commission pursuant to section 8(a) of the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8A, see the List of CFR Sections Affected, which... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.10 Form...

  13. 17 CFR 274.10 - Form N-8A, for notification of registration.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... filed with the Commission pursuant to section 8(a) of the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8A, see the List of CFR Sections Affected, which... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.10 Form...

  14. 48 CFR 52.219-18 - Notification of Competition Limited to Eligible 8(a) Concerns.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Competition Limited to Eligible 8(a) Concerns. 52.219-18 Section 52.219-18 Federal Acquisition Regulations... CLAUSES Text of Provisions and Clauses 52.219-18 Notification of Competition Limited to Eligible 8(a) Concerns. As prescribed in 19.811-3(d), insert the following clause: Notification of Competition Limited...

  15. 48 CFR 52.219-18 - Notification of Competition Limited to Eligible 8(a) Concerns.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Competition Limited to Eligible 8(a) Concerns. 52.219-18 Section 52.219-18 Federal Acquisition Regulations... CLAUSES Text of Provisions and Clauses 52.219-18 Notification of Competition Limited to Eligible 8(a) Concerns. As prescribed in 19.811-3(d), insert the following clause: Notification of Competition Limited...

  16. 15 CFR 8a.205 - Educational institutions and other entities controlled by religious organizations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... entities controlled by religious organizations. 8a.205 Section 8a.205 Commerce and Foreign Trade Office of... controlled by religious organizations. (a) Exemption. These Title IX regulations do not apply to any operation of an educational institution or other entity that is controlled by a religious organization...

  17. 13 CFR 124.514 - Exercise of 8(a) options and modifications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Exercise of 8(a) options and... Contractual Assistance § 124.514 Exercise of 8(a) options and modifications. (a) Unpriced options. The exercise of an unpriced option is considered to be a new contracting action. (1) If a concern has...

  18. 13 CFR 124.514 - Exercise of 8(a) options and modifications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Exercise of 8(a) options and... Contractual Assistance § 124.514 Exercise of 8(a) options and modifications. (a) Unpriced options. The exercise of an unpriced option is considered to be a new contracting action. (1) If a concern has...

  19. 13 CFR 124.514 - Exercise of 8(a) options and modifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Exercise of 8(a) options and... Contractual Assistance § 124.514 Exercise of 8(a) options and modifications. (a) Unpriced options. The exercise of an unpriced option is considered to be a new contracting action. (1) If a concern has...

  20. 13 CFR 124.514 - Exercise of 8(a) options and modifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Exercise of 8(a) options and... Contractual Assistance § 124.514 Exercise of 8(a) options and modifications. (a) Unpriced options. The exercise of an unpriced option is considered to be a new contracting action. (1) If a concern has...

  1. 13 CFR 124.514 - Exercise of 8(a) options and modifications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Exercise of 8(a) options and... Contractual Assistance § 124.514 Exercise of 8(a) options and modifications. (a) Unpriced options. The exercise of an unpriced option is considered to be a new contracting action. (1) If a concern has...

  2. 17 CFR 274.10 - Form N-8A, for notification of registration.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...: For Federal Register citations affecting Form N-8A, see the List of CFR Sections Affected, which... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Form N-8A, for notification of... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.10 Form...

  3. 17 CFR 274.10 - Form N-8A, for notification of registration.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...: For Federal Register citations affecting Form N-8A, see the List of CFR Sections Affected, which... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Form N-8A, for notification of... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.10 Form...

  4. 48 CFR 52.219-11 - Special 8(a) Contract Conditions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Special 8(a) Contract... (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 52.219-11 Special 8(a) Contract Conditions. As prescribed in 19.811-3(a), insert the following...

  5. 13 CFR 124.509 - What are non-8(a) business activity targets?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development..., business development, financing, marketing, accounting, or proposal preparation. (5) SBA may initiate... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What are non-8(a)...

  6. Involvement of calprotectin (S100A8/A9) in molecular pathways associated with HNSCC

    PubMed Central

    Khammanivong, Ali; Sorenson, Brent S.; Ross, Karen F.; Dickerson, Erin B.; Hasina, Rifat; Lingen, Mark W.; Herzberg, Mark C.

    2016-01-01

    Calprotectin (S100A8/A9), a heterodimeric protein complex of calcium-binding proteins S100A8 and S100A9, plays key roles in cell cycle regulation and inflammation, with potential functions in squamous cell differentiation. While upregulated in many cancers, S100A8/A9 is downregulated in squamous cell carcinomas of the cervix, esophagus, and the head and neck (HNSCC). We previously reported that ectopic S100A8/A9 expression inhibits cell cycle progression in carcinoma cells. Here, we show that declining expression of S100A8/A9 in patients with HNSCC is associated with increased DNA methylation, less differentiated tumors, and reduced overall survival. Upon ectopic over-expression of S100A8/A9, the cancer phenotype of S100A8/A9-negative carcinoma cells was suppressed in vitro and tumor growth in vivo was significantly decreased. MMP1, INHBA, FST, LAMC2, CCL3, SULF1, and SLC16A1 were significantly upregulated in HNSCC but were downregulated by S100A8/A9 expression. Our findings strongly suggest that downregulation of S100A8/A9 through epigenetic mechanisms may contribute to increased proliferation, malignant transformation, and disease progression in HNSCC. PMID:26883112

  7. Role of the hippocampus in Nav1.6 (Scn8a) mediated seizure resistance

    PubMed Central

    Lamar, Tyra; Goldin, Alan L; Escayg, Andrew

    2014-01-01

    SCN1A mutations are the main cause of the epilepsy disorders Dravet syndrome (DS) and genetic epilepsy with febrile seizures plus (GEFS+). Mutations that reduce the activity of the mouse Scn8a gene, in contrast, are found to confer seizure resistance and extend the lifespan of mouse models of DS and GEFS+. To investigate the mechanism by which reduced Scn8a expression confers seizure resistance, we induced interictal-like burst discharges in hippocampal slices of heterozygous Scn8a null mice (Scn8amed/+) with elevated extracellular potassium. Scn8amed/+ mutants exhibited reduced epileptiform burst discharge activity after P20, indicating an age-dependent increased threshold for induction of epileptiform discharges. Scn8a deficiency also reduced the occurrence of burst discharges in a GEFS+ mouse model (Scn1aR1648H/+). There was no detectable change in the expression levels of Scn1a (Nav1.1) or Scn2a (Nav1.2) in the hippocampus of adult Scn8amed/+ mutants. To determine whether the increased seizure resistance associated with reduced Scn8a expression was due to alterations that occurred during development, we examined the effect of deleting Scn8a in adult mice. Global Cre-mediated deletion of a heterozygous floxed Scn8a allele in adult mice was found to increase thresholds to chemically and electrically induced seizures. Finally, knockdown of Scn8a gene expression in the adult hippocampus via lentiviral Cre injection resulted in a reduction in the number of EEG-confirmed seizures following the administration of picrotoxin. Our results identify the hippocampus as an important structure in the mediation of Scn8a-dependent seizure protection and suggest that selective targeting of Scn8a activity might be efficacious in patients with epilepsy. PMID:24704313

  8. Alkyl nitrate formation from the reactions of C8-C14 n-alkanes with OH radicals in the presence of NO(x): measured yields with essential corrections for gas-wall partitioning.

    PubMed

    Yeh, Geoffrey K; Ziemann, Paul J

    2014-09-18

    In this study, C8-C14 n-alkanes were reacted with OH radicals in the presence of NO(x) in a Teflon film environmental chamber and isomer-specific yields of alkyl nitrates were determined using gas chromatography. Because results indicated significant losses of alkyl nitrates to chamber walls, gas-wall partitioning was investigated by monitoring the concentrations of a suite of synthesized alkyl nitrates added to the chamber. Gas-to-wall partitioning increased with increasing carbon number and with proximity of the nitrooxy group to the terminal carbon, with losses as high as 86%. The results were used to develop a structure-activity model to predict the effects of carbon number and isomer structure on gas-wall partitioning, which was used to correct the measured yields of alkyl nitrate isomers formed in chamber reactions. The resulting branching ratios for formation of secondary alkyl nitrates were similar for all isomers of a particular carbon number, and average values, which were almost identical to alkyl nitrate yields, were 0.219, 0.206, 0.254, 0.291, and 0.315 for reactions of n-octane, n-decane, n-dodecane, n-tridecane, and n-tetradecane, respectively. The increase in average branching ratios and alkyl nitrate yields with increasing carbon number to a plateau value of ∼0.30 at about C13-C14 is consistent with predictions of a previously developed model, indicating that the model is valid for alkane carbon numbers ≥C3.

  9. Alkyl nitrate formation from the reactions of C8-C14 n-alkanes with OH radicals in the presence of NO(x): measured yields with essential corrections for gas-wall partitioning.

    PubMed

    Yeh, Geoffrey K; Ziemann, Paul J

    2014-09-18

    In this study, C8-C14 n-alkanes were reacted with OH radicals in the presence of NO(x) in a Teflon film environmental chamber and isomer-specific yields of alkyl nitrates were determined using gas chromatography. Because results indicated significant losses of alkyl nitrates to chamber walls, gas-wall partitioning was investigated by monitoring the concentrations of a suite of synthesized alkyl nitrates added to the chamber. Gas-to-wall partitioning increased with increasing carbon number and with proximity of the nitrooxy group to the terminal carbon, with losses as high as 86%. The results were used to develop a structure-activity model to predict the effects of carbon number and isomer structure on gas-wall partitioning, which was used to correct the measured yields of alkyl nitrate isomers formed in chamber reactions. The resulting branching ratios for formation of secondary alkyl nitrates were similar for all isomers of a particular carbon number, and average values, which were almost identical to alkyl nitrate yields, were 0.219, 0.206, 0.254, 0.291, and 0.315 for reactions of n-octane, n-decane, n-dodecane, n-tridecane, and n-tetradecane, respectively. The increase in average branching ratios and alkyl nitrate yields with increasing carbon number to a plateau value of ∼0.30 at about C13-C14 is consistent with predictions of a previously developed model, indicating that the model is valid for alkane carbon numbers ≥C3. PMID:24654572

  10. COSMIC ORIGINS SPECTROGRAPH OBSERVATIONS OF TRANSLUCENT CLOUDS: Cyg OB2 8A

    SciTech Connect

    Snow, Theodore P.; Destree, Joshua D.; Burgh, Eric B.; Ferguson, Ryan M.; Danforth, Charles W.; Cordiner, Martin E-mail: destree@colorado.ed E-mail: ryan.m.ferguson@colorado.ed E-mail: martin.cordiner@nasa.go

    2010-09-10

    Data from the Cosmic Origins Spectrograph (COS) are presented for the first highly reddened target (Cyg OB2 8A) under the COS Science Team's guaranteed time allocation. Column densities of ionic, atomic, and molecular species are reported and implications are discussed. Data from Cyg OB2 8A demonstrate the ability to analyze highly reddened interstellar sight lines with the COS that were unavailable to previous UV instruments. Measured column densities indicate that the Cyg OB2 8A line of sight contains multiple diffuse clouds rather than a dominant translucent cloud.

  11. 7 CFR 6.2 - Responsibility for actions under section 22 and section 8(a).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... under section 22 of the Agricultural Adjustment Act of 1933, as amended, and section 8(a) of the Trade Agreements Extension Act of 1951 is assigned to the Administrator, Foreign Agricultural Service (referred...

  12. 7 CFR 6.2 - Responsibility for actions under section 22 and section 8(a).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... under section 22 of the Agricultural Adjustment Act of 1933, as amended, and section 8(a) of the Trade Agreements Extension Act of 1951 is assigned to the Administrator, Foreign Agricultural Service (referred...

  13. 48 CFR 1052.219-72 - Section 8(a) Direct Awards.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 1052.219-72... contracting activity and the 8(a) contractor pursuant to the Memorandum of Understanding between the...

  14. 48 CFR 1252.219-71 - Section 8(a) Direct Awards.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Section 8(a) Direct Awards. As prescribed in (TAR) 48 CFR 1219.811-3(f), insert the following clause... administration office. (c) The contractor agrees: (1) To notify the Contracting Officer, simultaneous with...

  15. 48 CFR 1252.219-71 - Section 8(a) Direct Awards.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Section 8(a) Direct Awards. As prescribed in (TAR) 48 CFR 1219.811-3(f), insert the following clause... administration office. (c) The contractor agrees: (1) To notify the Contracting Officer, simultaneous with...

  16. 11. A westward view within building #8A of space used ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. A westward view within building #8-A of space used as a foreman's office, as storage, and for rectifiers (converters of alternating current to direct current for the zinc-electro-plating equipment). Buildings #6-A and #8-A were the enclosed outside spaces between the outer walls of buildings #6 and #7, and #8 and #7 respectively. - American Chain & Cable Company, East Princess Street (400 Block), York, York County, PA

  17. 7 CFR 319.8a - Administrative instructions relating to the entry of cotton and covers into Guam.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... cotton and covers into Guam. 319.8a Section 319.8a Agriculture Regulations of the Department of... QUARANTINE NOTICES Foreign Cotton and Covers Quarantine § 319.8a Administrative instructions relating to the entry of cotton and covers into Guam. The plants and products specified in § 319.8(a) may be...

  18. 7 CFR 319.8a - Administrative instructions relating to the entry of cotton and covers into Guam.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... cotton and covers into Guam. 319.8a Section 319.8a Agriculture Regulations of the Department of... QUARANTINE NOTICES Foreign Cotton and Covers Quarantine § 319.8a Administrative instructions relating to the entry of cotton and covers into Guam. The plants and products specified in § 319.8(a) may be...

  19. 7 CFR 319.8a - Administrative instructions relating to the entry of cotton and covers into Guam.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... cotton and covers into Guam. 319.8a Section 319.8a Agriculture Regulations of the Department of... QUARANTINE NOTICES Foreign Cotton and Covers Quarantine § 319.8a Administrative instructions relating to the entry of cotton and covers into Guam. The plants and products specified in § 319.8(a) may be...

  20. 7 CFR 319.8a - Administrative instructions relating to the entry of cotton and covers into Guam.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... cotton and covers into Guam. 319.8a Section 319.8a Agriculture Regulations of the Department of... QUARANTINE NOTICES Foreign Cotton and Covers Quarantine § 319.8a Administrative instructions relating to the entry of cotton and covers into Guam. The plants and products specified in § 319.8(a) may be...

  1. 7 CFR 319.8a - Administrative instructions relating to the entry of cotton and covers into Guam.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... cotton and covers into Guam. 319.8a Section 319.8a Agriculture Regulations of the Department of... QUARANTINE NOTICES Foreign Cotton and Covers Quarantine § 319.8a Administrative instructions relating to the entry of cotton and covers into Guam. The plants and products specified in § 319.8(a) may be...

  2. 13 CFR 124.518 - How can an 8(a) contract be terminated before performance is completed?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Contractual Assistance § 124.518 How can an 8(a) contract be terminated before... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false How can an 8(a) contract...

  3. 13 CFR 124.506 - At what dollar threshold must an 8(a) procurement be competed among eligible Participants?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Contractual Assistance § 124.506 At what dollar threshold must an 8(a..., Subpart A, 8(a) Business Development, contains acquisition-related dollar thresholds subject...

  4. 13 CFR 124.510 - What percentage of work must a Participant perform on an 8(a) contract?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business...) contract? (a) To assist the business development of Participants in the 8(a) BD program, an 8(a) contractor... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What percentage of work must...

  5. 13 CFR 124.506 - At what dollar threshold must an 8(a) procurement be competed among eligible Participants?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Contractual Assistance § 124.506 At what dollar threshold must an 8(a..., Subpart A, 8(a) Business Development, contains acquisition-related dollar thresholds subject...

  6. 13 CFR 124.510 - What percentage of work must a Participant perform on an 8(a) contract?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business...) contract? (a) To assist the business development of Participants in the 8(a) BD program, an 8(a) contractor... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What percentage of work must...

  7. 13 CFR 124.518 - How can an 8(a) contract be terminated before performance is completed?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Contractual Assistance § 124.518 How can an 8(a) contract be terminated before... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false How can an 8(a) contract...

  8. Morphological, physiological and enzymatic characteristics of cephalosporin acylase-producing Arthrobacter strain 45-8A.

    PubMed

    Zhang, Q J; Xu, W X

    1993-01-01

    A bacterial strain producing cephalosporin acylases was isolated from soil. The morphological and physiological properties of this strain suggest that it belongs to the genus Arthrobacter, and the isolate was therefore designated Arthrobacter strain 45-8A. Substrate specificity of the enzyme was examined. The enzyme can convert both cephalosporin acid to 7-aminocephalosporanic acid. An interesting feature of the acylases is their temperature-dependent regulation. Activity of acylases was detected in strain 45-8A grown at temperature below 30 degrees C, but was not observed at higher temperature. Arthrobacter strain 45-8A did not exhibit beta-lactamase activity, even though its resistance to cephalosporin C was very strong (> 2000 micrograms/ml). This is quite beneficial for its application in the manufacture of 7-aminocephalosporanic acid. PMID:8484708

  9. Kinetic and Structural Studies of Phosphodiesterase-8A and Implication on the Inhibitor Selectivity

    SciTech Connect

    Wang, H.; Yan, Z; Yang, S; Cai, J; Robinson, H; Ke, H

    2008-01-01

    Cyclic nucleotide phosphodiesterase-8 (PDE8) is a family of cAMP-specific enzymes and plays important roles in many biological processes, including T-cell activation, testosterone production, adrenocortical hyperplasia, and thyroid function. However, no PDE8 selective inhibitors are available for trial treatment of human diseases. Here we report kinetic properties of the highly active PDE8A1 catalytic domain prepared from refolding and its crystal structures in the unliganded and 3-isobutyl-1-methylxanthine (IBMX) bound forms at 1.9 and 2.1 Angstroms resolutions, respectively. The PDE8A1 catalytic domain has a KM of 1.8 eM, Vmax of 6.1 emol/min/mg, a kcat of 4.0 s-1 for cAMP, and a KM of 1.6 mM, Vmax of 2.5 emol/min/mg, a kcat of 1.6 s-1 for cGMP, thus indicating that the substrate specificity of PDE8 is dominated by KM. The structure of the PDE8A1 catalytic domain has similar topology as those of other PDE families but contains two extra helices around Asn685-Thr710. Since this fragment is distant from the active site of the enzyme, its impact on the catalysis is unclear. The PDE8A1 catalytic domain is insensitive to the IBMX inhibition (IC50 = 700 eM). The unfavorable interaction of IBMX in the PDE8A1-IBMX structure suggests an important role of Tyr748 in the inhibitor binding. Indeed, the mutation of Tyr748 to phenylalanine increases the PDE8A1 sensitivity to several nonselective or family selective PDE inhibitors. Thus, the structural and mutagenesis studies provide not only insight into the enzymatic properties but also guidelines for design of PDE8 selective inhibitors.

  10. Influence of the availability of iron during hypoxia on the genes associated with apoptotic activity and local iron metabolism in rat H9C2 cardiomyocytes and L6G8C5 skeletal myocytes.

    PubMed

    Dziegala, Magdalena; Kasztura, Monika; Kobak, Kamil; Bania, Jacek; Banasiak, Waldemar; Ponikowski, Piotr; Jankowska, Ewa A

    2016-10-01

    The differential availability of iron during hypoxia is presumed to affect the functioning of cardiac and skeletal myocytes. Rat H9C2 cardiomyocytes and L6G8C5 myocytes were cultured for 48 h in normoxic or hypoxic conditions at the optimal, reduced or increased iron concentration. The mRNA expression levels of markers of apoptosis [B‑cell lymphoma‑2 (Bcl2; inhibition) and Bcl‑2‑activated X protein (Bax; induction)], atrophy (Atrogin), glycolysis (pyruvate kinase 2; PKM2) and iron metabolism [transferrin receptor 1 (TfR1; iron importer), ferroportin 1 (FPN1; iron exporter), ferritin heavy chain (FTH; iron storage protein) and hepcidin (HAMP; iron regulator)] were determined using reverse transcription‑quantitative polymerase chain reaction, and cell viability was measured using an tetrazolium reduction assay. Cardiomyocytes and myocytes, when exposed to hypoxia, demonstrated an increased Bax/Bcl‑2 gene expression ratio (P<0.05). Additional deferoxamine (DFO) treatment resulted in further increases in Bax/Bcl‑2 in each cell type (P<0.001 each) and this was associated with the 15% loss in viability. The analogous alterations were observed in both cell types upon ammonium ferric citrate (AFC) treatment during hypoxia; however, the increased Bax/Bcl‑2 ratio and associated viability loss was lower compared with that in case of DFO treatment (P<0.05 each). Under hypoxic conditions, myocytes demonstrated an increased expression of PKM2 (P<0.01). Additional DFO treatment caused an increase in the mRNA expression levels of PKM2 and Atrogin‑1 (P<0.001 and P<0.05, respectively), whereas AFC treatment caused an increased mRNA expression of PKM2 (P<0.01) and accompanied decreased mRNA expression of Atrogin‑1 (P<0.05). The expression augmentation of PKM2 during hypoxia was greater upon low iron compared with that of ferric salt treatment (P<0.01). Both cell types upon DFO during hypoxia demonstrated the increased expression of TfR1

  11. Influence of the availability of iron during hypoxia on the genes associated with apoptotic activity and local iron metabolism in rat H9C2 cardiomyocytes and L6G8C5 skeletal myocytes.

    PubMed

    Dziegala, Magdalena; Kasztura, Monika; Kobak, Kamil; Bania, Jacek; Banasiak, Waldemar; Ponikowski, Piotr; Jankowska, Ewa A

    2016-10-01

    The differential availability of iron during hypoxia is presumed to affect the functioning of cardiac and skeletal myocytes. Rat H9C2 cardiomyocytes and L6G8C5 myocytes were cultured for 48 h in normoxic or hypoxic conditions at the optimal, reduced or increased iron concentration. The mRNA expression levels of markers of apoptosis [B‑cell lymphoma‑2 (Bcl2; inhibition) and Bcl‑2‑activated X protein (Bax; induction)], atrophy (Atrogin), glycolysis (pyruvate kinase 2; PKM2) and iron metabolism [transferrin receptor 1 (TfR1; iron importer), ferroportin 1 (FPN1; iron exporter), ferritin heavy chain (FTH; iron storage protein) and hepcidin (HAMP; iron regulator)] were determined using reverse transcription‑quantitative polymerase chain reaction, and cell viability was measured using an tetrazolium reduction assay. Cardiomyocytes and myocytes, when exposed to hypoxia, demonstrated an increased Bax/Bcl‑2 gene expression ratio (P<0.05). Additional deferoxamine (DFO) treatment resulted in further increases in Bax/Bcl‑2 in each cell type (P<0.001 each) and this was associated with the 15% loss in viability. The analogous alterations were observed in both cell types upon ammonium ferric citrate (AFC) treatment during hypoxia; however, the increased Bax/Bcl‑2 ratio and associated viability loss was lower compared with that in case of DFO treatment (P<0.05 each). Under hypoxic conditions, myocytes demonstrated an increased expression of PKM2 (P<0.01). Additional DFO treatment caused an increase in the mRNA expression levels of PKM2 and Atrogin‑1 (P<0.001 and P<0.05, respectively), whereas AFC treatment caused an increased mRNA expression of PKM2 (P<0.01) and accompanied decreased mRNA expression of Atrogin‑1 (P<0.05). The expression augmentation of PKM2 during hypoxia was greater upon low iron compared with that of ferric salt treatment (P<0.01). Both cell types upon DFO during hypoxia demonstrated the increased expression of TfR1

  12. SED1/MFG-E8: a bi-motif protein that orchestrates diverse cellular interactions.

    PubMed

    Raymond, Adam; Ensslin, Michael A; Shur, Barry D

    2009-04-15

    MFG-E8 was initially identified as a principle component of the Milk Fat Globule, a membrane-encased collection of proteins and triglycerides that bud from the apical surface of mammary epithelia during lactation. It has since been independently identified in many species and by many investigators and given a variety of names, including p47, lactadherin, rAGS, PAS6/7, and BA-46. The acronym SED1 was proposed to bring cohesion to this nomenclature based upon it being a Secreted protein that contains two distinct functional domains: an N-terminal domain with two EGF-repeats, the second of which has an integrin-binding RGD motif, and a C-terminal domain with two Discoidin/F5/8C domains that bind to anionic phospholipids and/or extracellular matrices. SED1/MFG-E8 is now known to participate in a wide variety of cellular interactions, including phagocytosis of apoptotic lymphocytes and other apoptotic cells, adhesion between sperm and the egg coat, repair of intestinal mucosa, mammary gland branching morphogenesis, angiogenesis, among others. This article will explore the various roles proposed for SED1/MFG-E8, as well as its provocative therapeutic potential. PMID:19204935

  13. 13 CFR 124.511 - How is fair market price determined for an 8(a) contract?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... take into account prevailing market conditions, commercial prices for similar products or services, or... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false How is fair market price... Development Contractual Assistance § 124.511 How is fair market price determined for an 8(a) contract? (a)...

  14. 15 CFR 8a.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.550 Sex as...

  15. 15 CFR 8a.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.550 Sex as...

  16. 15 CFR 8a.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.550 Sex as...

  17. 15 CFR 8a.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.550 Sex as...

  18. 15 CFR 8a.550 - Sex as a bona fide occupational qualification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Sex as a bona fide occupational... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 8a.550 Sex as...

  19. 48 CFR 19.803 - Selecting acquisitions for the 8(a) Program.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... code, business support levels, and business activity targets; or, (ii) If competitive, a statement that... activity targets. If requested by the contracting activity, SBA will identify at least two such firms and... independently, or through the self marketing efforts of an 8(a) firm, identify a requirement for the...

  20. 15 CFR 8a.210 - Military and merchant marine educational institutions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Military and merchant marine... Coverage § 8a.210 Military and merchant marine educational institutions. These Title IX regulations do not apply to an educational institution whose primary purpose is the training of individuals for a...

  1. 15 CFR 8a.210 - Military and merchant marine educational institutions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Military and merchant marine... Coverage § 8a.210 Military and merchant marine educational institutions. These Title IX regulations do not apply to an educational institution whose primary purpose is the training of individuals for a...

  2. 15 CFR 8a.210 - Military and merchant marine educational institutions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Military and merchant marine... Coverage § 8a.210 Military and merchant marine educational institutions. These Title IX regulations do not apply to an educational institution whose primary purpose is the training of individuals for a...

  3. 15 CFR 8a.440 - Health and insurance benefits and services.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.440 Health and insurance... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Health and insurance benefits...

  4. 15 CFR 8a.440 - Health and insurance benefits and services.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Health and insurance benefits and... Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.440 Health and insurance... the basis of sex, or provide such benefit, service, policy, or plan in a manner that would...

  5. 15 CFR 8a.440 - Health and insurance benefits and services.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Health and insurance benefits and... Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.440 Health and insurance... the basis of sex, or provide such benefit, service, policy, or plan in a manner that would...

  6. 15 CFR 8a.440 - Health and insurance benefits and services.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Health and insurance benefits and... Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.440 Health and insurance... the basis of sex, or provide such benefit, service, policy, or plan in a manner that would...

  7. 76 FR 27859 - 8(a) Business Development Program Regulation Changes; Tribal Consultation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-13

    .... to 4 p.m. at the National 8(a) Association Summer Conference in the Dena'ina Civic and Convention... email chequita.carter@sba.gov . SUPPLEMENTARY INFORMATION: I. Background On February 11, 2011 (76 FR 8222), SBA issued a Final Rule, publicly available at...

  8. 13 CFR 124.507 - What procedures apply to competitive 8(a) procurements?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Participants in accordance with the Federal Acquisition Regulation (48 CFR, chapter 1). (b) Eligibility... paragraph (d)(1). The program term for 8(a) Participant X is scheduled to expire on December 19. A... procuring activity amends the solicitation to extend the date for the receipt of offers to January 5....

  9. 15 CFR 8a.210 - Military and merchant marine educational institutions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Military and merchant marine... Coverage § 8a.210 Military and merchant marine educational institutions. These Title IX regulations do not... service of the United States or for the merchant marine....

  10. 15 CFR 8a.110 - Remedial and affirmative action and self-evaluation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Remedial and affirmative action and... Introduction § 8a.110 Remedial and affirmative action and self-evaluation. (a) Remedial action. If the... official deems necessary to overcome the effects of such discrimination. (b) Affirmative action. In...

  11. 15 CFR 8a.110 - Remedial and affirmative action and self-evaluation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Remedial and affirmative action and... Introduction § 8a.110 Remedial and affirmative action and self-evaluation. (a) Remedial action. If the... official deems necessary to overcome the effects of such discrimination. (b) Affirmative action. In...

  12. 15 CFR 8a.110 - Remedial and affirmative action and self-evaluation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Remedial and affirmative action and... Introduction § 8a.110 Remedial and affirmative action and self-evaluation. (a) Remedial action. If the... official deems necessary to overcome the effects of such discrimination. (b) Affirmative action. In...

  13. 15 CFR 8a.110 - Remedial and affirmative action and self-evaluation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Remedial and affirmative action and... Introduction § 8a.110 Remedial and affirmative action and self-evaluation. (a) Remedial action. If the... official deems necessary to overcome the effects of such discrimination. (b) Affirmative action. In...

  14. 15 CFR 8a.110 - Remedial and affirmative action and self-evaluation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Remedial and affirmative action and... Introduction § 8a.110 Remedial and affirmative action and self-evaluation. (a) Remedial action. If the... official deems necessary to overcome the effects of such discrimination. (b) Affirmative action. In...

  15. 15 CFR 8a.440 - Health and insurance benefits and services.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Health and insurance benefits and... benefits and services. Subject to § 8a.235(d), in providing a medical, hospital, accident, or life insurance benefit, service, policy, or plan to any of its students, a recipient shall not discriminate...

  16. 76 FR 14559 - Federal Acquisition Regulation; Justification and Approval of Sole-Source 8(a) Contracts

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-16

    ... the meeting notice that was published in the Federal Register on August 31, 2010 at 75 FR 53269... Acquisition Regulation; Justification and Approval of Sole-Source 8(a) Contracts AGENCIES: Department of...) sole-source contracts over $20 million, as required by section 811. DATES: Effective Date: March...

  17. 15 CFR 8a.420 - Access to schools operated by LEAs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 8a.420 Access to schools... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Access to schools operated by LEAs....

  18. Convulsive seizures and SUDEP in a mouse model of SCN8A epileptic encephalopathy.

    PubMed

    Wagnon, Jacy L; Korn, Matthew J; Parent, Rachel; Tarpey, Taylor A; Jones, Julie M; Hammer, Michael F; Murphy, Geoffrey G; Parent, Jack M; Meisler, Miriam H

    2015-01-15

    De novo mutations of the voltage-gated sodium channel gene SCN8A have recently been recognized as a cause of epileptic encephalopathy, which is characterized by refractory seizures with developmental delay and cognitive disability. We previously described the heterozygous SCN8A missense mutation p.Asn1768Asp in a child with epileptic encephalopathy that included seizures, ataxia, and sudden unexpected death in epilepsy (SUDEP). The mutation results in increased persistent sodium current and hyperactivity of transfected neurons. We have characterized a knock-in mouse model expressing this dominant gain-of-function mutation to investigate the pathology of the altered channel in vivo. The mutant channel protein is stable in vivo. Heterozygous Scn8a(N1768D/+) mice exhibit seizures and SUDEP, confirming the causality of the de novo mutation in the proband. Using video/EEG analysis, we detect ictal discharges that coincide with convulsive seizures and myoclonic jerks. Prior to seizure onset, heterozygous mutants are not defective in motor learning or fear conditioning, but do exhibit mild impairment of motor coordination and social discrimination. Homozygous mutant mice exhibit earlier seizure onset than heterozygotes and more rapid progression to death. Analysis of the intermediate phenotype of functionally hemizygous Scn8a(N1768D/-) mice indicates that severity is increased by a double dose of mutant protein and reduced by the presence of wild-type protein. Scn8a(N1768D) mutant mice provide a model of epileptic encephalopathy that will be valuable for studying the in vivo effects of hyperactive Nav1.6 and the response to therapeutic interventions.

  19. Relationship between TMEM16A/anoctamin 1 and LRRC8A.

    PubMed

    Benedetto, Roberta; Sirianant, Lalida; Pankonien, Ines; Wanitchakool, Podchanart; Ousingsawat, Jiraporn; Cabrita, Ines; Schreiber, Rainer; Amaral, Margarida; Kunzelmann, Karl

    2016-10-01

    TMEM16A/anoctamin 1/ANO1 and VRAC/LRRC8 are independent chloride channels activated either by increase in intracellular Ca(2+) or cell swelling, respectively. In previous studies, we observed overlapping properties for both types of channels. (i) TMEM16A/ANO1 and LRRC8 are inhibited by identical compounds, (ii) the volume-regulated anion channel VRAC requires compartmentalized Ca(2+) increase to be fully activated, (iii) anoctamins are activated by cell swelling, (iv) both channels have a role for apoptotic cell death, (v) both channels are possibly located in lipid rafts/caveolae like structures, and (vi) VRAC and anoctamin 1 currents are not additive when each are fully activated. In the present study, we demonstrate in different cell types that loss of LRRC8A expression not only inhibited VRAC, but also attenuated Ca(2+) activated Cl(-) currents. Moreover, expression of LRRC8A enhanced Ca(2+) activated Cl(-) currents, and both LRRC8A and ANO1 could be coimmunoprecipitated. We found that LRRC8A becomes accessible to biotinylation upon exposure to hypotonic bath solution, while membrane capacitance was not enhanced. When intracellular Ca(2+) was increased in ANO1-expressing cells, the membrane capacitance was enhanced and increased binding of FM4-64 to the membrane was observed. As this was not seen in cells lacking ANO1 expression, a role of ANO1 for exocytosis was suggested. We propose that ANO1 and LRRC8A are activated in parallel. Thus, ionomycin or purinergic stimulation will not only activate ANO1 but also LRRC8 currents. Cell swelling will not only activate LRRC8/VRAC, but also stimulate ANO1 currents by enhancing compartmentalized Ca(2+) increase and/or through swelling induced autocrine release of ATP. PMID:27514381

  20. Relationship between TMEM16A/anoctamin 1 and LRRC8A.

    PubMed

    Benedetto, Roberta; Sirianant, Lalida; Pankonien, Ines; Wanitchakool, Podchanart; Ousingsawat, Jiraporn; Cabrita, Ines; Schreiber, Rainer; Amaral, Margarida; Kunzelmann, Karl

    2016-10-01

    TMEM16A/anoctamin 1/ANO1 and VRAC/LRRC8 are independent chloride channels activated either by increase in intracellular Ca(2+) or cell swelling, respectively. In previous studies, we observed overlapping properties for both types of channels. (i) TMEM16A/ANO1 and LRRC8 are inhibited by identical compounds, (ii) the volume-regulated anion channel VRAC requires compartmentalized Ca(2+) increase to be fully activated, (iii) anoctamins are activated by cell swelling, (iv) both channels have a role for apoptotic cell death, (v) both channels are possibly located in lipid rafts/caveolae like structures, and (vi) VRAC and anoctamin 1 currents are not additive when each are fully activated. In the present study, we demonstrate in different cell types that loss of LRRC8A expression not only inhibited VRAC, but also attenuated Ca(2+) activated Cl(-) currents. Moreover, expression of LRRC8A enhanced Ca(2+) activated Cl(-) currents, and both LRRC8A and ANO1 could be coimmunoprecipitated. We found that LRRC8A becomes accessible to biotinylation upon exposure to hypotonic bath solution, while membrane capacitance was not enhanced. When intracellular Ca(2+) was increased in ANO1-expressing cells, the membrane capacitance was enhanced and increased binding of FM4-64 to the membrane was observed. As this was not seen in cells lacking ANO1 expression, a role of ANO1 for exocytosis was suggested. We propose that ANO1 and LRRC8A are activated in parallel. Thus, ionomycin or purinergic stimulation will not only activate ANO1 but also LRRC8 currents. Cell swelling will not only activate LRRC8/VRAC, but also stimulate ANO1 currents by enhancing compartmentalized Ca(2+) increase and/or through swelling induced autocrine release of ATP.

  1. Loss of col8a1a function during zebrafish embryogenesis results in congenital vertebral malformations.

    PubMed

    Gray, Ryan S; Wilm, Thomas P; Smith, Jeff; Bagnat, Michel; Dale, Rodney M; Topczewski, Jacek; Johnson, Stephen L; Solnica-Krezel, Lilianna

    2014-02-01

    Congenital vertebral malformations (CVM) occur in 1 in 1000 live births and in many cases can cause spinal deformities, such as scoliosis, and result in disability and distress of affected individuals. Many severe forms of the disease, such as spondylocostal dystostosis, are recessive monogenic traits affecting somitogenesis, however the etiologies of the majority of CVM cases remain undetermined. Here we demonstrate that morphological defects of the notochord in zebrafish can generate congenital-type spine defects. We characterize three recessive zebrafish leviathan/col8a1a mutant alleles ((m531, vu41, vu105)) that disrupt collagen type VIII alpha1a (col8a1a), and cause folding of the embryonic notochord and consequently adult vertebral column malformations. Furthermore, we provide evidence that a transient loss of col8a1a function or inhibition of Lysyl oxidases with drugs during embryogenesis was sufficient to generate vertebral fusions and scoliosis in the adult spine. Using periodic imaging of individual zebrafish, we correlate focal notochord defects of the embryo with vertebral malformations (VM) in the adult. Finally, we show that bends and kinks in the notochord can lead to aberrant apposition of osteoblasts normally confined to well-segmented areas of the developing vertebral bodies. Our results afford a novel mechanism for the formation of VM, independent of defects of somitogenesis, resulting from aberrant bone deposition at regions of misshapen notochord tissue.

  2. Loss of col8a1a Function during Zebrafish Embryogenesis Results in Congenital Vertebral Malformations

    PubMed Central

    Gray, Ryan S.; Wilm, Thomas; Smith, Jeff; Bagnat, Michel; Dale, Rodney M.; Topczewski, Jacek; Johnson, Stephen L.; Solnica-Krezel, Lilianna

    2014-01-01

    Congenital vertebral malformations (CVM) occur in 1 in 1,000 live births and in many cases can cause spinal deformities, such as scoliosis, and result in disability and distress of affected individuals. Many severe forms of the disease, such as spondylocostal dystostosis, are recessive monogenic traits affecting somitogenesis, however the etiologies of the majority of CVM cases remain undetermined. Here we demonstrate that morphological defects of the notochord in zebrafish can generate congenital-type spine defects. We characterize three recessive zebrafish leviathan/col8a1a mutant alleles (m531, vu41, vu105) that disrupt collagen type VIII alpha1a (col8a1a), and cause folding of the embryonic notochord and consequently adult vertebral column malformations. Furthermore, we provide evidence that a transient loss of col8a1a function or inhibition of Lysyl oxidases with drugs during embryogenesis was sufficient to generate vertebral fusions and scoliosis in the adult spine. Using periodic imaging of individual zebrafish, we correlate focal notochord defects of the embryo with vertebral malformations (VM) in the adult. Finally, we show that bends and kinks in the notochord can lead to aberrant apposition of osteoblasts normally confined to well-segmented areas of the developing vertebral bodies. Our results afford a novel mechanism for the formation of VM, independent of defects of somitogenesis, resulting from aberrant bone deposition at regions of misshapen notochord tissue. PMID:24333517

  3. Exposure of CCRF-CEM cells to acridone derivative 8a triggers tumor death via multiple mechanisms.

    PubMed

    Wang, Yini; Gao, Dan; Chu, Bizhu; Gao, Chunmei; Cao, Deliang; Liu, Hongxia; Jiang, Yuyang

    2016-04-01

    A newly synthesized acridone derivative 8a shows potent antitumor activity against CCRF-CEM leukemia cells. Herein, the first proteomic study of 8a effects in CCRF-CEM cells was performed by 2D nano-LC-ESI-MS/MS to better understand the mechanisms of action of 8a. Data analyses based on PLGS, STRING, Cytoscape, and database for annotation, visualization, and integrated discovery identified 55 proteins that were differentially expressed in response to 8a exposure. Multiple cellular pathways were affected, including chromatin organization, energy metabolism, DNA repair, oxidative-stress, and apoptosis. The changes in protein expression were further verified for PKM2. Moreover, 8a lowered down the expression of HEX and PFK-1. Lactate production was decreased in 8a-treated cells, indicating suppression of glycolysis. The elevated XRCC6 and decreased histone expression levels suggested increased DNA damage in 8a-treated cells, which was confirmed by the increased γ-H2AX foci. Molecular docking of 8a with DNA demonstrated direct interactions of 8a with DNA through three hydrogen bonds and four π-π interactions, potentially explaining the mode of action that 8a damaged to DNA. The differential protein profiling and dysfunction of metabolic pathways induced by 8a provide novel insights into the potential action mechanisms of 8a.

  4. 13 CFR 124.203 - What must a concern submit to apply to the 8(a) BD program?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What must a concern submit to apply to the 8(a) BD program? 124.203 Section 124.203 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a)...

  5. 13 CFR 124.101 - What are the basic requirements a concern must meet for the 8(a) BD program?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Eligibility Requirements for Participation in the 8(a) Business Development Program... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What are the basic requirements...

  6. 13 CFR 124.102 - What size business is eligible to participate in the 8(a) BD program?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Eligibility Requirements for Participation in the 8(a) Business Development Program § 124.102 What... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What size business is eligible...

  7. 13 CFR 124.101 - What are the basic requirements a concern must meet for the 8(a) BD program?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Eligibility Requirements for Participation in the 8(a) Business Development Program... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What are the basic requirements...

  8. 13 CFR 124.102 - What size business is eligible to participate in the 8(a) BD program?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Eligibility Requirements for Participation in the 8(a) Business Development Program § 124.102 What... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What size business is eligible...

  9. 15 CFR 8b.23 - Housing provided by the recipient.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... of living accommodations is, as a whole, comparable to that of nonhandicapped students. (b) Other... of its students shall take such action as may be necessary to assure itself that such housing is, as a whole, made available in a manner that does not result in discrimination on the basis of handicap....

  10. 17 CFR 270.8b-2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... statements, means certified by an independent public or independent certified public accountant or accountants. (c) Charter. The term “charter” includes articles of incorporation, declaration of trust, articles of association or partnership, or any similar instrument, as amended, effecting (either with...

  11. 17 CFR 270.8b-2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... accountants. (c) Charter. The term “charter” includes articles of incorporation, declaration of trust... of the advisory board. (e) Fiscal year. The term “fiscal year” means the annual accounting period...

  12. 15 CFR 8b.20 - Admission and recruitment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...); (ii) admissions tests that are designed for persons with impaired sensory, manual, or speaking skills... are handicapped, Provided, That: (1) The recipient states clearly on any written questionnaire used for this purpose or makes clear orally, if no written questionnaire is used, that the...

  13. 15 CFR 8b.20 - Admission and recruitment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... speaking skills, the test results accurately reflect the applicant's aptitude or achievement level of..., manual, or speaking skills (except where those skills are the factors that the test purports to measure); (ii) admissions tests that are designed for persons with impaired sensory, manual, or speaking...

  14. Phosphorylation status of human RNA-binding protein 8A in cells and its inhibitory regulation by Magoh

    PubMed Central

    Nakamura, Yuka; Tatsuno, Takanori; Ma, Shaofu; Tomosugi, Naohisa

    2015-01-01

    The RNA-binding protein 8A (RBM8A)–mago-nashi homolog, proliferation-associated (Magoh) complex is a component of the exon junction complex (EJC) required for mRNA metabolism involving nonsense-mediated mRNA decay (NMD). RBM8A is a phosphorylated protein that plays some roles in NMD. However, the detailed status and mechanism of the phosphorylation of RBM8A is not completely understood. Therefore, in this study, we analyzed in detail RBM8A phosphorylation in human cells. Accordingly, analysis of the phosphorylation status of RBM8A protein in whole-cell lysates by using Phos-tag gels revealed that the majority of endogenous RBM8A was phosphorylated throughout the cell-cycle progression. Nuclear and cytoplasmic RBM8A and RBM8A in the EJC were also found to be mostly phosphorylated. We also screened the phosphorylated serine by mutational analysis using Phos-tag gels to reveal modifications of serine residues 166 and 168. A single substitution at position 168 that concomitantly abolished the phosphorylation of serine 166 suggested the priority of kinase reaction between these sites. Furthermore, analysis of the role of the binding protein Magoh in RBM8A phosphorylation revealed its inhibitory effect in vitro and in vivo. Thus, we conclude that almost all synthesized RBM8A proteins are rapidly phosphorylated in cells and that phosphorylation occurs before the complex formation with Magoh. PMID:25349214

  15. Structure and properties of ITQ-8: a hydrous layer silicate with microporous silicate layers.

    PubMed

    Marler, Bernd; Müller, Melanie; Gies, Hermann

    2016-06-21

    ITQ-8 is a new hydrous layer silicate (HLS) with a chemical composition of [C4H8(C7H13N)2]8 [Si64O128(OH)16]·48H2O per unit cell. The synthesis of ITQ-8 was first described in 2002 by Díaz-Cabañas et al., the structure of this material, however, remained unsolved at that time. Physico-chemical characterization using solid-state NMR spectroscopy, SEM, TG-DTA, and FTIR spectroscopy confirmed that ITQ-8 is a layer silicate. The XRD powder pattern was indexed in the monoclinic system with lattice parameters of a0 = 35.5168(5) Å, b0 = 13.3989(2) Å, c0 = 16.0351(2) Å, β = 106.74(2)°. The crystal structure was solved by simulated annealing. Rietveld refinement of the structure in space group C2/c converged to residual values of RBragg = 0.023, RF = 0.022 and chi(2) = 2.3 confirming the structure model. The structure of ITQ-8 contains silicate layers with a topology that resembles a (11-1) section of the framework of zeolite levyne. So far, this layer topology is unique among layer silicates. The layer can be regarded as made up of 4-, 6-, double-six and 8-rings which are interconnected to form cup-like "half-cages". Unlike other HLSs, which possess impermeable silicate layers, ITQ-8 contains 8-rings pores with a free diameter of 3.5 Å × 3.4 Å and can be regarded as a "small-pore layer silicate". In the crystal structure, the organic cations, 1,4-diquiniclidiniumbutane, used as structure directing agents during synthesis are intercalated between the silicate layers. Clusters (bands) of water molecules which are hydrogen bonded to each other and to the terminal Si-OH/Si-O(-) groups are located between the organic cations and interconnect the silicate layers. ITQ-8 is a very interesting material as precursor for the synthesis of microporous framework silicates by topotactic condensation or interlayer expansion reactions leading to 3D micro-pore systems which may be useful in applications as e.g. catalysts, catalyst supports and adsorbents of for separation. PMID

  16. Crystal structures of the novel hydrated borates Ba{sub 2}B{sub 5}O{sub 9}(OH), Sr{sub 2}B{sub 5}O{sub 9}(OH) and Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2}

    SciTech Connect

    McMillen, Colin; Heyward, Carla; Giesber, Henry; Kolis, Joseph

    2011-11-15

    Three novel hydrated borates Ba{sub 2}B{sub 5}O{sub 9}(OH) (1), Sr{sub 2}B{sub 5}O{sub 9}(OH) (2) and Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2} (3) have been synthesized hydrothermally and their structures determined. Compounds (1) and (2) are isostructural, crystallizing in space group P2{sub 1}/c and having lattice parameters of a=6.6330(13) A, b=8.6250(17) A, c=14.680(3) A, {beta}=93.46(3){sup o} and a=6.4970(13) A, b=8.4180(17) A, c=14.177(3) A, {beta}=94.35(3){sup o}, respectively. Compound (3) crystallizes in P-1 with lattice parameters of a=6.4684(13) A, b=8.4513(17) A, c=14.881(3) A, {alpha}=101.21(3){sup o}, {beta}=93.96(3){sup o}, {gamma}=90.67(3){sup o}. While similar in their axis lengths, (3) differs greatly in structure and formulation from (1) and (2). The structure of (1) and (2) is contrasted to that of the well-known mineral hilgardite (Ca{sub 2}B{sub 5}O{sub 9}Cl.H{sub 2}O). - Graphical abstract: Novel hydrated borate structures. Highlights: > Synthesis and crystal structures of three novel hydrated borates are reported. > Unique descriptive chemistry governing Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2} synthesis is discussed. > Structures of Ba{sub 2}B{sub 5}O{sub 9}(OH) and Sr{sub 2}B{sub 5}O{sub 9}(OH) are contrasted to Ca{sub 2}B{sub 5}O{sub 9}Cl.H{sub 2}O. > Structure of Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2} differs from Ba{sub 2}B{sub 5}O{sub 9}(OH) and Sr{sub 2}B{sub 5}O{sub 9}(OH).

  17. Thermal fatigue and oxidation data of TAZ-8A and M22 alloys and variations

    NASA Technical Reports Server (NTRS)

    Hofer, K. E.; Humphreys, V. E.

    1981-01-01

    Thermal fatigue and oxidation data were obtained on 36 specimens, representing 18 distinct variations (including the base systems) of TAZ-8A and M22 alloys. Double-edge wedge specimens for these systems were cycled between fluidized beds maintained at 1088 C and 316 C with a 180 s immersion in each bed. The systems included alloys TAZ-8A, M22, and 16 variations of these alloys. Each alloy variation consisted of a unique composition with an alternation in the percentage of carbon (C1 and C2), molydenum (M1 and M2), tungsten (W1 and W2), columbium (CB1, CB2, and CB3), tantalium (T1, T2, and T3), or boron (B1, B2, and B3) present. All of the alloys showed little weight change due to oxidation compared with other alloys previously tested in fluidized beds. Only both C1 alloy variation specimens survived 3500 cycles without cracking in the small radius, although substantial cracks were present, emanating from the end notches which were used for holding the specimens.

  18. Quorum sensing activity of Citrobacter amalonaticus L8A, a bacterium isolated from dental plaque

    PubMed Central

    Goh, Share-Yuan; Khan, Saad Ahmed; Tee, Kok Keng; Abu Kasim, Noor Hayaty; Yin, Wai-Fong; Chan, Kok-Gan

    2016-01-01

    Cell-cell communication is also known as quorum sensing (QS) that happens in the bacterial cells with the aim to regulate their genes expression in response to increased cell density. In this study, a bacterium (L8A) isolated from dental plaque biofilm was identified as Citrobacter amalonaticus by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Its N-acylhomoserine-lactone (AHL) production was screened by using two types of AHL biosensors namely Chromobacterium violaceum CV026 and Escherichia coli [pSB401]. Citrobacter amalonaticus strain L8A was identified and confirmed producing numerous types of AHL namely N-butyryl-L-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL) and N-hexadecanoyl-L-homoserine lactone (C16-HSL). We performed the whole genome sequence analysis of this oral isolate where its genome sequence reveals the presence of QS signal synthase gene and our work will pave the ways to study the function of the related QS genes in this bacterium. PMID:26860259

  19. Aberrant hippocampal Atp8a1 levels are associated with altered synaptic strength, electrical activity, and autistic-like behavior.

    PubMed

    Kerr, Daniel J; Marsillo, Alexandra; Guariglia, Sara R; Budylin, Tatyana; Sadek, Rodina; Menkes, Silvia; Chauhan, Abha; Wen, Guang Y; McCloskey, Daniel P; Wieraszko, Andrzej; Banerjee, Probal

    2016-09-01

    Type IV ATPases are putative aminophospholipid translocases (APLTs), more commonly known as flippases. A pronounced induction of the flippase Atp8a1 was observed in post-mortem tissue homogenates from the hippocampus and temporal lobe of juvenile autistic subjects compared to age-matched controls. In order to simulate the human data, C57BL/6 mice were allowed to develop after intra-hippocampal injection of recombinant lentivirus expressing Atp8a1 at the early developmental stage of postnatal day 6 (P6). Transmission electron microscopy (TEM) analysis of the lentivirus-Atp8a1 treated (Atp8a1+) mice in adulthood revealed fewer and weaker excitatory synapses in the hippocampal CA1 region compared to mice injected with empty virus. Significant inhibition of the Schaffer collateral pathway was observed in the Atp8a1+ mice in paired-pulse recording (PPR) at 20-ms inter-stimulus interval. In the three-chambered sociability test, the Atp8a1+ mice displayed no preference for an encaged stranger mouse over a novel object, which is a characteristic autistic-like behavior. In sharp contrast, Atp8a1 (-/-) mice displayed a preference for a stranger mouse over the novel object, which is characteristic of neurotypical mouse behavior. However, similar to the Atp8a1+ mice, the Atp8a1 (-/-) mice harbored fewer and weaker excitatory synapses in CA1 compared to wild-type controls, and displayed inhibition at 20-ms inter-stimulus interval in PPR. These findings suggest that both elevated and diminished levels of Atp8a1 during early development are detrimental to brain connectivity, but only elevated Atp8a1 is associated with aberrant social behavior. Mice with augmented levels of Atp8a1 may therefore serve as a potential model in autism research. PMID:27287255

  20. Test of acoustic tone source and propulsion performance of C8A Buffalo suppressor nozzle

    NASA Technical Reports Server (NTRS)

    Marrs, C. C.; Harkonen, D. L.; Okeefe, J. V.

    1974-01-01

    Results are presented for a static acoustic and propulsion performance ground test conducted at the Boeing hot nozzle facility on the C8A Buffalo noise suppressor nozzle. Various methods to remove a nozzle-associated 2000-Hz tone are evaluated. Results of testing this rectangular-array lobed nozzle for propulsion performance and acoustic directivity are reported. Recommendations for future nozzle modifications and further testing are included. Appendix A contains the test plan. Appendix B presents the test log. Appendix C contains plots of the one-third octave sound pressure levels recorded during the test. Appendix D describes the acoustic data recording and reduction systems. The performance data is tabulated in Appendix E.

  1. Palladium-catalyzed cross-coupling reactions of 4a,8a-azaboranaphthalene.

    PubMed

    Sun, Feiye; Lv, Lily; Huang, Min; Zhou, Zhaohui; Fang, Xiangdong

    2014-10-01

    A concise and effective three-step synthesis of 4a,8a-azaboranaphthalene (ABN) has been developed in gram scale. Electrophilic aromatic substitution reactions of ABN provide excellent functional-group-tolerant cross-coupling partners in various Pd-catalyzed cross-coupling reactions (e.g., Sonogashira, Suzuki-Miyaura, or Heck reaction). Photophysical, electrochemical, and DFT calculations all suggest a narrowed HOMO-LUMO gap with extended π-conjugation characters in the cross-coupled molecules. The ABN moiety as a new fluorophore has a distinct and selective fluorescence response toward Zn(II) and Cd(II) ions, demonstrating great potential for the ABN structural motif in fluorescent chemosensors.

  2. Aerosol data assimilation using data from Himawari-8, a next-generation geostationary meteorological satellite

    NASA Astrophysics Data System (ADS)

    Yumimoto, K.; Nagao, T. M.; Kikuchi, M.; Sekiyama, T. T.; Murakami, H.; Tanaka, T. Y.; Ogi, A.; Irie, H.; Khatri, P.; Okumura, H.; Arai, K.; Morino, I.; Uchino, O.; Maki, T.

    2016-06-01

    Himawari-8, a next-generation geostationary meteorological satellite, was launched on 7 October 2014 and became operational on 7 July 2015. The advanced imager on board Himawari-8 is equipped with 16 observational bands (including three visible and three near-infrared bands) that enable retrieval of full-disk aerosol optical properties at 10 min intervals from geostationary (GEO) orbit. Here we show the first application of aerosol optical properties (AOPs) derived from Himawari-8 data to aerosol data assimilation. Validation of the assimilation experiment by comparison with independent observations demonstrated successful modeling of continental pollution that was not predicted by simulation without assimilation and reduced overestimates of dust front concentrations. These promising results suggest that AOPs derived from Himawari-8/9 and other planned GEO satellites will considerably improve forecasts of air quality, inverse modeling of emissions, and aerosol reanalysis through assimilation techniques.

  3. [Effect of heat shock on cells of phytopathogenic mycoplasma Acholeplasma laidlawii PG-8A].

    PubMed

    Vishniakov, I E; Levitskiĭ, S A; Borkhsenius, S N

    2015-01-01

    Heat shock caused a more active formation of the "dormant" forms (minibodies), as well as increased production of extracellular membrane vesicles by Acholeplasma laidlawii PG-8A cells. Raise of the amount of the minibodies that have increased resistance to biogenic and abiogenic stress factors and pathogenicity may lead to more successful persistence of mycoplasmas in their hosts. Increased production of the extracellular membrane vesicles containing virulence factors by Acholeplasma laidlawii cells during stress may be an additional burden for the infected organism. It has been recently revealed that the vesicles of A. laidlawii contain appreciable quantities of small heat shock protein IbpA (Hsp20). In this paper, using immune-electron microscopy, have shown that at elevated temperature IbpA is associated with A. laidlawii minibodies. Perhaps, IbpA contributes to increased resistance and pathogenicity of the minibodies, keeping their proteins and polypeptides, including protein virulence factors in the folding-competent state.

  4. 17 CFR 239.15 - Form N-1 for open-end management investment companies registered on Form N-8A.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... affecting Form N-1, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... section 2(a)(37) of the Investment Company Act of 1940 registered under the Investment Company Act of 1940... of such companies pursuant to section 8(b) of the Investment Company Act of 1940 (§ 274.11 of...

  5. 17 CFR 239.15 - Form N-1 for open-end management investment companies registered on Form N-8A.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... affecting Form N-1, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... section 2(a)(37) of the Investment Company Act of 1940 registered under the Investment Company Act of 1940... of such companies pursuant to section 8(b) of the Investment Company Act of 1940 (§ 274.11 of...

  6. 17 CFR 239.15 - Form N-1 for open-end management investment companies registered on Form N-8A.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... affecting Form N-1, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... section 2(a)(37) of the Investment Company Act of 1940 registered under the Investment Company Act of 1940... of such companies pursuant to section 8(b) of the Investment Company Act of 1940 (§ 274.11 of...

  7. 17 CFR 239.15 - Form N-1 for open-end management investment companies registered on Form N-8A.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... affecting Form N-1, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... section 2(a)(37) of the Investment Company Act of 1940 registered under the Investment Company Act of 1940... of such companies pursuant to section 8(b) of the Investment Company Act of 1940 (§ 274.11 of...

  8. 17 CFR 239.15 - Form N-1 for open-end management investment companies registered on Form N-8A.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... affecting Form N-1, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... section 2(a)(37) of the Investment Company Act of 1940 registered under the Investment Company Act of 1940... of such companies pursuant to section 8(b) of the Investment Company Act of 1940 (§ 274.11 of...

  9. 13 CFR 124.513 - Under what circumstances can a joint venture be awarded an 8(a) contract?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    .... (2) Where a joint venture has been established and approved by SBA for one 8(a) contract, a second or... agreement, setting forth the performance requirements on that second or third contract, is provided to and... activity will execute an 8(a) contract in the name of the joint venture entity. (g) Amendments to...

  10. 13 CFR 124.305 - What is suspension and how is a Participant suspended from the 8(a) BD program?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... those which have been “self-marketed” by a Participant, will not be made during the pendency of the... requirements that the firm has self-marketed and those that have been accepted into the 8(a) BD program on... 8(a) contracts. (i) SBA does not recognize the concept of de facto suspension. Adding time to...

  11. 13 CFR 124.305 - What is suspension and how is a Participant suspended from the 8(a) BD program?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... those which have been “self-marketed” by a Participant, will not be made during the pendency of the... requirements that the firm has self-marketed and those that have been accepted into the 8(a) BD program on... 8(a) contracts. (i) SBA does not recognize the concept of de facto suspension. Adding time to...

  12. 13 CFR 124.305 - What is suspension and how is a Participant suspended from the 8(a) BD program?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... those which have been “self-marketed” by a Participant, will not be made during the pendency of the... requirements that the firm has self-marketed and those that have been accepted into the 8(a) BD program on... 8(a) contracts. (i) SBA does not recognize the concept of de facto suspension. Adding time to...

  13. 13 CFR 124.2 - What length of time may a business participate in the 8(a) BD program?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Provisions of General Applicability § 124.2 What length of time may a business participate in the... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false What length of time may a...

  14. 13 CFR 124.513 - Under what circumstances can a joint venture be awarded an 8(a) contract?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... BUSINESS ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Contractual Assistance § 124.513 Under what circumstances can a joint venture be... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Under what circumstances can...

  15. 13 CFR 124.2 - What length of time may a business participate in the 8(a) BD program?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ADMINISTRATION 8(a) BUSINESS DEVELOPMENT/SMALL DISADVANTAGED BUSINESS STATUS DETERMINATIONS 8(a) Business Development Provisions of General Applicability § 124.2 What length of time may a business participate in the... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What length of time may a...

  16. α-Synuclein interacts with the switch region of Rab8a in a Ser129 phosphorylation-dependent manner.

    PubMed

    Yin, Guowei; Lopes da Fonseca, Tomas; Eisbach, Sibylle E; Anduaga, Ane Martín; Breda, Carlo; Orcellet, Maria L; Szegő, Éva M; Guerreiro, Patricia; Lázaro, Diana F; Braus, Gerhard H; Fernandez, Claudio O; Griesinger, Christian; Becker, Stefan; Goody, Roger S; Itzen, Aymelt; Giorgini, Flaviano; Outeiro, Tiago F; Zweckstetter, Markus

    2014-10-01

    Alpha-synuclein (αS) misfolding is associated with Parkinson's disease (PD) but little is known about the mechanisms underlying αS toxicity. Increasing evidence suggests that defects in membrane transport play an important role in neuronal dysfunction. Here we demonstrate that the GTPase Rab8a interacts with αS in rodent brain. NMR spectroscopy reveals that the C-terminus of αS binds to the functionally important switch region as well as the C-terminal tail of Rab8a. In line with a direct Rab8a/αS interaction, Rab8a enhanced αS aggregation and reduced αS-induced cellular toxicity. In addition, Rab8 - the Drosophila ortholog of Rab8a - ameliorated αS-oligomer specific locomotor impairment and neuron loss in fruit flies. In support of the pathogenic relevance of the αS-Rab8a interaction, phosphorylation of αS at S129 enhanced binding to Rab8a, increased formation of insoluble αS aggregates and reduced cellular toxicity. Our study provides novel mechanistic insights into the interplay of the GTPase Rab8a and αS cytotoxicity, and underscores the therapeutic potential of targeting this interaction.

  17. 13 CFR 124.513 - Under what circumstances can a joint venture be awarded an 8(a) contract?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... joint venture be awarded an 8(a) contract? 124.513 Section 124.513 Business Credit and Assistance SMALL...) Business Development Contractual Assistance § 124.513 Under what circumstances can a joint venture be... the purpose of performing one or more specific 8(a) contracts. (2) A joint venture agreement...

  18. 48 CFR 1352.219-72 - Notification of competition limited to eligible 8(a) concerns, Alternate III (Deviation).

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... eligible 8(a) concerns, Alternate III (Deviation). As prescribed in 48 CFR 1319.811-3 (c), insert the...) (APR 2010) (a) Offers are solicited only from small business concerns expressly certified by the Small Business Administration (SBA) for participation in the SBA's 8(a) Program and which meet the...

  19. Structure of apo-azurin from Alcaligenes denitrificans at 1.8 A resolution.

    PubMed

    Shepard, W E; Kingston, R L; Anderson, B F; Baker, E N

    1993-05-01

    The structure of apo-azurin from Alcaligenes denitrificans has been determined at high resolution by X-ray crystallography. Two separate structure analyses have been carried out, (i) on crystals obtained from solutions of apo-azurin and (ii) on crystals obtained by removal of copper from previously formed crystals of holo-azurin. Data to 1.8 A resolution were collected from the apo-azurin crystals, by Weissenberg photography (with image plates) using synchrotron radiation and by diffractometry, and the structure was refined by restrained least-squares methods to a final R value of 0.160 for all data in the range 10.0-1.8 A. The final model of 1954 protein atoms, 246 water molecules (66 half-weighted), four SO(4)(2-) ions, and two low-occupancy (0.13 and 0.15) Cu atoms has r.m.s. deviations of 0.012, 0.045 and 0.013 A from standard bond lengths, angle distances and planar groups. For copper-removed azurin, data to 2.2 A were collected by diffractometry and the structure refined by restrained least squares to a final R value of 0.158 for all data in the range 10.0-2.2 A. The final model of 1954 protein atoms, 264 water molecules, two SO(4)(2-) ions, two low occupancy (0.18 and 0.22) metal atoms and one unidentified atom (modelled as S) has r.m.s. deviations of 0.013, 0.047 and 0.012 A from standard bond lengths, angle distances and planar groups. The two structures are essentially identical to each other and show no significant differences from the oxidized and reduced holo-azurin structures. The ligand side chains move slightly closer together following the removal of copper, with the radius of the cavity between the three strongly binding ligands, His 46, His 117 and Cys 112, shrinking from 1.31 A in reduced azurin to 1.24 A in oxidized azurin and 1.16 A in apo-azurin. There is a suggestion of increased flexibility in one of the copper-binding loops but the structure supports the view that the copper site found in holo-azurin is a stable structure, defined by the

  20. Myosin Va mediates Rab8A-regulated GLUT4 vesicle exocytosis in insulin-stimulated muscle cells.

    PubMed

    Sun, Yi; Chiu, Tim T; Foley, Kevin P; Bilan, Philip J; Klip, Amira

    2014-04-01

    Rab-GTPases are important molecular switches regulating intracellular vesicle traffic, and we recently showed that Rab8A and Rab13 are activated by insulin in muscle to mobilize GLUT4-containing vesicles to the muscle cell surface. Here we show that the unconventional motor protein myosin Va (MyoVa) is an effector of Rab8A in this process. In CHO-IR cell lysates, a glutathione S-transferase chimera of the cargo-binding COOH tail (CT) of MyoVa binds Rab8A and the related Rab10, but not Rab13. Binding to Rab8A is stimulated by insulin in a phosphatidylinositol 3-kinase-dependent manner, whereas Rab10 binding is insulin insensitive. MyoVa-CT preferentially binds GTP-locked Rab8A. Full-length green fluorescent protein (GFP)-MyoVa colocalizes with mCherry-Rab8A in perinuclear small puncta, whereas GFP-MyoVa-CT collapses the GTPase into enlarged perinuclear depots. Further, GFP-MyoVa-CT blocks insulin-stimulated translocation of exofacially myc-tagged GLUT4 to the surface of muscle cells. Mutation of amino acids in MyoVa-CT predicted to bind Rab8A abrogates both interaction with Rab8A (not Rab10) and inhibition of insulin-stimulated GLUT4myc translocation. Of importance, small interfering RNA-mediated MyoVa silencing reduces insulin-stimulated GLUT4myc translocation. Rab8A colocalizes with GLUT4 in perinuclear but not submembrane regions visualized by confocal total internal reflection fluorescence microscopy. Hence insulin signaling to the molecular switch Rab8A connects with the motor protein MyoVa to mobilize GLUT4 vesicles toward the muscle cell plasma membrane.