Science.gov

Sample records for 8b 8c 8d

  1. Multiple proton decays of 6Be, 8C, 8B(IAS) and excited states in 10C

    NASA Astrophysics Data System (ADS)

    Sobotka, Lee

    2011-10-01

    Recent technical advances have allowed for high-order correlation experiments to be done. We have primarily focused on experiments in which the final channels are composed of only alphas and protons. Four cases we have studied are: 6Be, 10C*, 8C, and 8B*(IAS) via 3, 4, 5, and 3-particle correlation measurements, respectively. While the first case had been studied before, our work presents very high statistics in the full Jacobi coordinates (the coordinates needed to describe 3-body decay.) Our study of 10C excited states provides isolatable examples of: correlated 2p decay, from one state, and the decay of another which is unusually highly correlated, a ``ménage a quatre.'' 8C decay presents the only case of sequential 3-body 2p decay steps (i.e. 2p-2p.) The intermediate in this 2-step process is the first example (6Be) mentioned above. Unlike the well-studied second step (6Be decay), the first step in this 2p-2p process provides another example of correlated 2p emission. The decay of 8B(IAS), the isobaric analog of 8C, also decays overwhelmingly by 2p emission, in this case to 6Li(IAS). This IAS-to-IAS 2p decay is one for which decay to the potential 1p intermediates is energetically allowed but isospin forbidden. This represents an expansion, over that originally envisioned by Goldanski, of the conceivable nuclear territory for 2p decay.

  2. The Protein Synthesis Inhibitor Blasticidin S Enters Mammalian Cells via Leucine-rich Repeat-containing Protein 8D

    PubMed Central

    Lee, Clarissa C.; Freinkman, Elizaveta; Sabatini, David M.; Ploegh, Hidde L.

    2014-01-01

    Leucine-rich repeat-containing 8 (LRRC8) proteins have been identified as putative receptors involved in lymphocyte development and adipocyte differentiation. They remain poorly characterized, and no specific function has been assigned to them. There is no consensus on how this family of proteins might function because homology searches suggest that members of the LRRC8 family act not as plasma membrane receptors, but rather as channels that mediate cell-cell signaling. Here we provide experimental evidence that supports a role for LRRC8s in the transport of small molecules. We show that LRRC8D is a mammalian protein required for the import of the antibiotic blasticidin S. We characterize localization and topology of LRRC8A and LRRC8D and demonstrate that LRRC8D interacts with LRRC8A, LRRC8B, and LRRC8C. Given the suggested involvement in solute transport, our results support a model in which LRRC8s form one or more complexes that may mediate cell-cell communication by transporting small solutes. PMID:24782309

  3. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  4. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  5. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  6. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  7. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  8. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  9. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  10. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  11. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  12. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  13. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  14. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  15. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  16. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... activities. (2) A recipient may offer to handicapped students physical education and athletic activities that... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students,...

  17. 15 CFR 8b.14 - Preemployment inquiries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 8b.14 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... applicant is a handicapped individual, or as to the nature or severity of a handicap. A recipient may... a recipient is taking remedial action to correct the effects of past discrimination pursuant to §...

  18. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  19. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  20. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  1. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  2. JT8D engine performance retention

    NASA Technical Reports Server (NTRS)

    James, A. D.; Weisel, D. R.

    1981-01-01

    The attractive performance retention characteristics of the JT8D engine are described. Because of its moderate bypass ratio and turbine temperature, and stiff structural design, the performance retention versus flight cycles of the JT8D engine sets a standard that is difficult for other engines to equal. In addition, the significant benefits of refurbishment of the JT8D engine are presented. Cold section refurbishment offers thrust specific fuel consumption improvements of up to 2 percent and payback in less than a year, making a very attractive investment option for the airlines.

  3. Foothills Parkway Section 8B Final Environmental Report, Volume 2, Appendices A-C

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NW, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this EN there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constricting Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER, which consists of Appendices A, B, and C, assesses the potential geologic impacts of the proposed Section 8B construction, presents the results of the Section 8B soil survey, and describes the water quality studies and analyses performed for the ER. The following summary sections provide information for geology, soils, and water quality.

  4. 15 CFR 8c.60 - Communications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Communications. 8c.60 Section 8c.60 Commerce and Foreign Trade Office of the Secretary of Commerce ENFORCEMENT OF NONDISCRIMINATION ON THE... Communications. (a) The agency shall take appropriate steps to ensure effective communication with...

  5. 15 CFR 8c.60 - Communications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Communications. 8c.60 Section 8c.60 Commerce and Foreign Trade Office of the Secretary of Commerce ENFORCEMENT OF NONDISCRIMINATION ON THE... Communications. (a) The agency shall take appropriate steps to ensure effective communication with...

  6. 15 CFR 8c.60 - Communications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Communications. 8c.60 Section 8c.60 Commerce and Foreign Trade Office of the Secretary of Commerce ENFORCEMENT OF NONDISCRIMINATION ON THE... Communications. (a) The agency shall take appropriate steps to ensure effective communication with...

  7. West Valley Tank 8D-1 and 8D-2 Inventory Estimation Methodology

    SciTech Connect

    O'Brien, Robert F.; Heasler, Patrick G.; Rowell, Laurene

    2001-07-20

    This report details work funded by the West Valley Support Project (WVSP) and the Tanks Focus Area Retrieval and Closure Program. The work was conducted by the Pacific Northwest National Laboratory (PNNL) and is in support of the West Valley Demonstration Project (WVDP). The WVDP site in New York was originally the site of a commercial nuclear fuel reprocessing plant. The high-level waste (HLW), approximately 2 million liters, produced during plutonium-uranium extraction (PUREX) and thorium extraction (THOREX) reprocessing campaigns at the plant and subsequent HLW preprocessing, was stored on site in three tanks identified as 8D-1, 8D-2, and 8D-4. Waste from the PUREX process was neutralized with NaOH for storage in a carbon steel tank designated as 8D-2. Neutralization resulted in a precipitated hydroxide sludge that settled to the bottom of the tank and was covered by a supernatant salt solution. The acidic THOREX waste, approximately 55,000 L, was first stored in a stainless steel tank (8D-4) and then added to the PUREX waste in Tank 8D-2. Supernatant decontamination, primarily cesium removal, was conducted by ion-exchange using in-tank columns suspended in Tank 8D-1. The cesium-loaded zeolite, resulting from the supernatant decontamination process, was dumped to the bottom of Tank 8D-1. Approximately 90% of the spent zeolite was transferred from Tank 8D-1 into Tank 8D-2 by the start of vitrification processing in June 1996. Periodically, the remaining spent zeolite contained in Tank 8D-1 is incrementally transferred to 8D-2. The combined waste in Tank 8D-2 continues to be processed through the Vitrification Facility into canisters for final repository disposal.

  8. An 8-b Josephson digital signal processor

    NASA Astrophysics Data System (ADS)

    Kotani, Seigo; Inoue, Atsuki; Imamura, Takeshi; Hasuo, Shinya

    1990-12-01

    A 6.3 K-gate Josephson digital signal processor (DSP) that performs 240-psec 8-b multiplication and 410-psec 13-b addition is described. The structure of the DSP, which is based on a three-stage pipeline, and the design of the components used in the DSP are reviewed. The DSP contains 23,000 Josephson junctions on a 5 x 5-mm die and was fabricated using 1.5-micron all-niobium Josephson techniques. Measurements indicate that the DSP can attain a nonparallel processing speed of 1 gigaoperation per second with 12-mW power dissipation.

  9. Foothills Parkway Section 8B Final Environmental Report, Volume 6, Appendix N

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER documents the results of the architectural, historical, and cultural resources assessment for the entire Section 8B ROW that was completed in May 1995 to document the architectural, historical, and cultural resources located within the project area. The assessment included evaluation of the potential for cultural (i.e., rural historic) landscapes in the area of the ROW. The assessment showed that one National Register-listed property is located 0.3 mile south of the ROW

  10. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Employment on ships. 8b.15 Section 8b... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license... employment on ships under any program or activity to which this part applies....

  11. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Employment on ships. 8b.15 Section 8b... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license... employment on ships under any program or activity to which this part applies....

  12. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Employment on ships. 8b.15 Section 8b... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license... employment on ships under any program or activity to which this part applies....

  13. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Employment on ships. 8b.15 Section 8b... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license... employment on ships under any program or activity to which this part applies....

  14. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Employment on ships. 8b.15 Section 8b... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license... employment on ships under any program or activity to which this part applies....

  15. Foothills Parkway Section 8B Final Environmental Report, Volume 5, Appendices J-M

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER consists of Appendices J through M, which describe potential impacts regarding traffic, noise, and aesthetics. The results of the traffic studies described in these appendices resulted in the following conclusions: Unacceptable levels of service will occur on numerous roads within and outside of the park increasingly in the Mure based upon current and future regional growth. However, the results of the traffic assessment indicated that there would be no significant or

  16. Foothills Parkway Section 8B Final Environmental Report, Volume 3, Appendix D

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER inventories the fishes and benthic macroinvertebrates inhabiting the aquatic ecosystems potentially affected by the proposed construction of Section 8B. Stream biological surveys were completed at 31 stream sites during the Fall of 1994. The sampling strategy for both invertebrates and fish was to survey the different taxa from all available habitats. For benthic invertebrates, a standardized qualitative manual collection technique was employed for all 31 stations. For fish

  17. F-8C adaptive flight control laws

    NASA Technical Reports Server (NTRS)

    Hartmann, G. L.; Harvey, C. A.; Stein, G.; Carlson, D. N.; Hendrick, R. C.

    1977-01-01

    Three candidate digital adaptive control laws were designed for NASA's F-8C digital flyby wire aircraft. Each design used the same control laws but adjusted the gains with a different adaptative algorithm. The three adaptive concepts were: high-gain limit cycle, Liapunov-stable model tracking, and maximum likelihood estimation. Sensors were restricted to conventional inertial instruments (rate gyros and accelerometers) without use of air-data measurements. Performance, growth potential, and computer requirements were used as criteria for selecting the most promising of these candidates for further refinement. The maximum likelihood concept was selected primarily because it offers the greatest potential for identifying several aircraft parameters and hence for improved control performance in future aircraft application. In terms of identification and gain adjustment accuracy, the MLE design is slightly superior to the other two, but this has no significant effects on the control performance achievable with the F-8C aircraft. The maximum likelihood design is recommended for flight test, and several refinements to that design are proposed.

  18. Foothills Parkway Section 8B Final Environmental Report, Volume 4, Appendices E-I

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical, resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER consists of Appendices E through I (all ecological survey reports), which are summarized individually in the sections that follow. The following conclusions result from the completion of these surveys and the ER impact analysis: (1) Forest clearing should be limited as much as possible; (2) Disturbed areas should be replanted with native trees; (3) Drainages should be bridged rather than leveled with cut and fill; (4) For areas of steep slopes and potential erosion

  19. 17 CFR 270.8b-3 - Title of securities.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 17 Commodity and Securities Exchanges 3 2013-04-01 2013-04-01 false Title of securities. 270.8b-3 Section 270.8b-3 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-3 Title of securities. Wherever the title of securities is required to be stated,...

  20. 17 CFR 270.8b-3 - Title of securities.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 17 Commodity and Securities Exchanges 4 2014-04-01 2014-04-01 false Title of securities. 270.8b-3 Section 270.8b-3 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-3 Title of securities. Wherever the title of securities is required to be stated,...

  1. 17 CFR 270.8b-3 - Title of securities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Title of securities. 270.8b-3 Section 270.8b-3 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-3 Title of securities. Wherever the title of securities is required to be stated,...

  2. 17 CFR 270.8b-3 - Title of securities.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 17 Commodity and Securities Exchanges 3 2012-04-01 2012-04-01 false Title of securities. 270.8b-3 Section 270.8b-3 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-3 Title of securities. Wherever the title of securities is required to be stated,...

  3. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Treatment of students. 8b.21 Section... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall, on... recipient to which this subpart applies that considers participation by students in education programs...

  4. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Treatment of students. 8b.21 Section... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall, on... recipient to which this subpart applies that considers participation by students in education programs...

  5. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Treatment of students. 8b.21 Section... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall, on... recipient to which this subpart applies that considers participation by students in education programs...

  6. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Treatment of students. 8b.21 Section... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall, on... recipient to which this subpart applies that considers participation by students in education programs...

  7. 17 CFR 270.8b-3 - Title of securities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Title of securities. 270.8b-3 Section 270.8b-3 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) RULES... value, if any; the rate of dividends, if fixed, and whether cumulative or noncumulative; a...

  8. Foothills Parkway Section 8B Final Environmental Report, Volume 1

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west.

  9. 15 CFR 8c.10 - Self-evaluation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Self-evaluation. 8c.10 Section 8c.10... BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF COMMERCE § 8c.10 Self... handicaps or organizations representing individuals with handicaps, to participate in the...

  10. 8B+27Al scattering at low energies

    NASA Astrophysics Data System (ADS)

    Morcelle, V.; Lichtenthäler, R.; Lépine-Szily, A.; Guimarães, V.; Pires, K. C. C.; Lubian, J.; Mendes Junior, D. R.; de Faria, P. N.; Kolata, J. J.; Becchetti, F. D.; Jiang, H.; Aguilera, E. F.; Lizcano, D.; Martinez-Quiroz, E.; Garcia, H.

    2017-01-01

    We present 8B 27Al elastic scattering angular distributions for the proton-halo nucleus 8B at two energies above the Coulomb barrier, namely Elab=15.3 and 21.7 MeV. The experiments were performed in the Radioactive Ion Beams in Brasil facility (RIBRAS) in São Paulo, and in the TwinSol facility at the University of Notre Dame, USA. The angular distributions were measured in the angular range of 15-80 degrees. Optical model and continuum discretized coupled channels calculations were performed, and the total reaction cross sections were derived. A comparison of the 8B+27Al total reaction cross sections with similar systems including exotic, weakly bound, and tightly bound projectiles impinging on the same target is presented.

  11. 8B studied as a secondary beam at GANIL

    NASA Astrophysics Data System (ADS)

    Borcea, C.; Carstoiu, F.; Negoita, F.; Lewitowicz, M.; Saint-Laurent, M. G.; Anne, R.; Bazin, D.; Borrel, V.; Corre, J. M.; Dlouhy, Z.; Fomitchev, A.; Guillemaud-Mueller, D.; Keller, H.; Kordyasz, A.; Lukyanov, S.; Mueller, A. C.; Penionzhkevich, Yu.; Roussel-Chomaz, P.; Skobelev, N.; Sorlin, O.; Tarasov, O.

    1997-02-01

    The LISE spectrometer at GANIL has been used to form a 8B secondary beam. Using a multiple silicon telescope, data have been obtained for the reaction and break-up cross sections, as well as for the parallel momentum distribution of 7Be from break-up. For the first time, separate contributions to the break-up of diffraction and/or Coulomb dissociation and absorption mechanisms have been determined. The ensemble of data supports the existence in 8B of a "pigmy" halo.

  12. JT8D-100 turbofan engine, phase 1. [noise reduction

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The JT8D turbofan engine, widely used in short and medium range transport aircraft, contributes substantially to airport community noise. The jet noise is predominant in the JT8D engine and may be reduced in a modified engine, without loss of thrust, by increasing the airflow to reduce jet velocity. A configuration study evaluated the effects of fan airflow, fan pressure ratio, and bypass ratio on noise, thrust, and fuel comsumption. The cycle selected for the modified engine was based upon an increased diameter, single-stage fan and two additional core engine compressor stages, which replace the existing two-stage fan. Modifications were also made to the low pressure turbine to provide the increased torque required by the larger diameter fan. The resultant JT8D-100 engine models have the following characteristics at take-off thrust, compared to the current JT8D engine: Airflow and bypass ratio are increased, and fan pressure ratio and engine speed are reduced. The resultant engine is also longer, larger in diameter, and heavier than the JT8D base model, but these latter changes are compensated by the increased thrust and decreased fuel comsumption of the modified engine, thus providing the capability for maintaining the performance of the current JT8D-powered aircraft.

  13. Precise determination of the unperturbed 8B neutrino spectrum.

    PubMed

    Roger, T; Büscher, J; Bastin, B; Kirsebom, O S; Raabe, R; Alcorta, M; Äystö, J; Borge, M J G; Carmona-Gallardo, M; Cocolios, T E; Cruz, J; Dendooven, P; Fraile, L M; Fynbo, H O U; Galaviz, D; Gasques, L R; Giri, G S; Huyse, M; Hyldegaard, S; Jungmann, K; Kruithof, W L; Lantz, M; Perea, A; Riisager, K; Saastamoinen, A; Santra, B; Shidling, P D; Sohani, M; Sørensen, A J; Tengblad, O; Traykov, E; van der Hoek, D J; Van Duppen, P; Versolato, O O; Wilschut, H W

    2012-04-20

    A measurement of the final state distribution of the (8)B β decay, obtained by implanting a (8)B beam in a double-sided silicon strip detector, is reported here. The present spectrum is consistent with a recent independent precise measurement performed by our collaboration at the IGISOL facility, Jyväskylä [O. S. Kirsebom et al., Phys. Rev. C 83, 065802 (2011)]. It shows discrepancies with previously measured spectra, leading to differences in the derived neutrino spectrum. Thanks to a low detection threshold, the neutrino spectrum is for the first time directly extracted from the measured final state distribution, thus avoiding the uncertainties related to the extrapolation of R-matrix fits. Combined with the IGISOL data, this leads to an improvement of the overall errors and the extension of the neutrino spectrum at high energy. The new unperturbed neutrino spectrum represents a benchmark for future measurements of the solar neutrino flux as a function of energy.

  14. Fungicidal mechanisms of the antimicrobial peptide Bac8c.

    PubMed

    Lee, Wonyoung; Lee, Dong Gun

    2015-02-01

    Bac8c (RIWVIWRR-NH2) is an analogue peptide derived through complete substitution analysis of the linear bovine host defense peptide variant Bac2A. In the present study, the antifungal mechanism of Bac8c against pathogenic fungi was investigated, with a particular focus on the effects of Bac8c on the cytoplasmic membrane. We used bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC4(3)] staining and 3,3'-dipropylthiacarbocyanine iodide [DiSC3(5)] assays to show that Bac8c induced disturbances in the membrane potential of Candida albicans. An increase in membrane permeability and suppression of cell wall regeneration were also observed in Bac8c-treated C. albicans. We studied the effects of Bac8c treatment on model membranes to elucidate its antifungal mechanism. Using calcein and FITC-labeled dextran leakage assays from Bac8c-treated large unilamellar vesicles (LUVs) and giant unilamellar vesicles (GUVs), we found that Bac8c has a pore-forming action on fungal membranes, with an estimated pore radius of between 2.3 and 3.3 nm. A membrane-targeted mechanism of action was also supported by the observation of potassium release from the cytosol of Bac8c-treated C. albicans. These results indicate that Bac8c is considered as a potential candidate to develop a novel antimicrobial agent because of its low-cost production characteristics and high antimicrobial activity via its ability to induce membrane perturbations in fungi.

  15. Peer review in Clinical Pharmacology using the 8-D Assessment

    PubMed Central

    Woodcock, Barry G.

    2017-01-01

    The requirement for editors of clinical pharmacology journals to maintain an overview of the peer review process for manuscripts submitted can be facilitated by use of the 8-D Assessment. The 8-D Assessment comprises peer review criteria to determine if the:1. Design of the study, 2. Diagnoses employed, 3. Drug molecules involved, 4. Dosages applied, 5. Data collected, 6. Discussion of the findings, 7. Deductions made, and 8. Documentation are in accord with the objectives of the study and meet the requirements of evidence-based medicine. This tool, although easy to apply, requires a high level of clinical pharmacology expertise, especially in the fields of drug disposition, pharmacokinetics, and drug action. PMID:28218890

  16. WASP-12b AND HAT-P-8b are members of triple star systems

    SciTech Connect

    Bechter, Eric B.; Crepp, Justin R.; Matthews, Christopher T.; Ngo, Henry; Knutson, Heather A.; Batygin, Konstantin; Johnson, John Asher; Hinkley, Sasha; Muirhead, Philip S.; Montet, Benjamin T.; Morton, Timothy D.; Howard, Andrew W.

    2014-06-10

    We present high spatial resolution images that demonstrate that WASP-12b and HAT-P-8b orbit the primary stars of hierarchical triple star systems. In each case, two distant companions with colors and brightnesses consistent with M dwarfs co-orbit the hot Jupiter planet host as well as one another. Our adaptive optics images spatially resolve the secondary around WASP-12, previously identified by Bergfors et al. and Crossfield et al. into two distinct sources separated by 84.3 ± 0.6 mas (21 ± 3 AU). We find that the secondary to HAT-P-8, also identified by Bergfors et al., is in fact composed of two stars separated by 65.3 ± 0.5 mas (15 ± 1 AU). Our follow-up observations demonstrate physical association through common proper motion. HAT-P-8 C has a particularly low mass, which we estimate to be 0.18 ± 0.02 M {sub ☉} using photometry. Due to their hierarchy, WASP-12 BC and HAT-P-8 BC will enable the first dynamical mass determination for hot Jupiter stellar companions. These previously well studied planet hosts now represent higher-order multi-star systems with potentially complex dynamics, underscoring the importance of diffraction-limited imaging and providing additional context for understanding the migrant population of transiting hot Jupiters.

  17. WASP-12b and HAT-P-8b are Members of Triple Star Systems

    NASA Astrophysics Data System (ADS)

    Bechter, Eric B.; Crepp, Justin R.; Ngo, Henry; Knutson, Heather A.; Batygin, Konstantin; Hinkley, Sasha; Muirhead, Philip S.; Johnson, John Asher; Howard, Andrew W.; Montet, Benjamin T.; Matthews, Christopher T.; Morton, Timothy D.

    2014-06-01

    We present high spatial resolution images that demonstrate that WASP-12b and HAT-P-8b orbit the primary stars of hierarchical triple star systems. In each case, two distant companions with colors and brightnesses consistent with M dwarfs co-orbit the hot Jupiter planet host as well as one another. Our adaptive optics images spatially resolve the secondary around WASP-12, previously identified by Bergfors et al. and Crossfield et al. into two distinct sources separated by 84.3 ± 0.6 mas (21 ± 3 AU). We find that the secondary to HAT-P-8, also identified by Bergfors et al., is in fact composed of two stars separated by 65.3 ± 0.5 mas (15 ± 1 AU). Our follow-up observations demonstrate physical association through common proper motion. HAT-P-8 C has a particularly low mass, which we estimate to be 0.18 ± 0.02 M ⊙ using photometry. Due to their hierarchy, WASP-12 BC and HAT-P-8 BC will enable the first dynamical mass determination for hot Jupiter stellar companions. These previously well studied planet hosts now represent higher-order multi-star systems with potentially complex dynamics, underscoring the importance of diffraction-limited imaging and providing additional context for understanding the migrant population of transiting hot Jupiters.

  18. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids are... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance....

  19. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids are... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance....

  20. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids are... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance....

  1. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids are... 40 Protection of Environment 24 2011-07-01 2011-07-01 false Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance....

  2. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Ammonium salts of higher fatty acids... Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids...

  3. JT8D high pressure compressor performance improvement

    NASA Technical Reports Server (NTRS)

    Gaffin, W. O.

    1981-01-01

    An improved performance high pressure compressor with potential application to all models of the JT8D engine was designed. The concept consisted of a trenched abradable rubstrip which mates with the blade tips for each of the even rotor stages. This feature allows tip clearances to be set so blade tips run at or near the optimum radius relative to the flowpath wall, without the danger of damaging the blades during transients and maneuvers. The improved compressor demonstrated thrust specific fuel consumption and exhaust gas temperature improvements of 1.0 percent and at least 10 C over the takeoff and climb power range at sea level static conditions, compared to a bill-of-material high pressure compressor. Surge margin also improved 4 percentage points over the high power operating range. A thrust specific fuel consumption improvement of 0.7 percent at typical cruise conditions was calculated based on the sea level test results.

  4. JT8D high pressure compressor performance improvement

    SciTech Connect

    Gaffin, W.O.

    1981-11-01

    An improved performance high pressure compressor with potential application to all models of the JT8D engine was designed. The concept consisted of a trenched abradable rubstrip which mates with the blade tips for each of the even rotor stages. This feature allows tip clearances to be set so blade tips run at or near the optimum radius relative to the flowpath wall, without the danger of damaging the blades during transients and maneuvers. The improved compressor demonstrated thrust-specific fuel consumption and exhaust gas temperature improvements of 1.0% and at least 10/sup 0/C over the takeoff and climb power range at sea level static conditions, compared to a bill-of-material high pressure compressor. Surge margin also improved 4 percentage points over the high power operating range. A thrust-specific fuel consumption improvement of 0.7% at typical cruise conditions was calculated based on the sea level test results.

  5. Extremal black attractors in 8D maximal supergravity

    SciTech Connect

    Drissi, L. B; Hassani, F. Z; Jehjouh, H.; Saidi, E. H

    2010-05-15

    Motivated by the new higher D-supergravity solutions on intersecting attractors obtained by Ferrara et al. in [Phys. Rev. D 79, 065031 (2009)], we focus in this paper on 8D maximal supergravity with moduli space (SL(3,R)/SO(3))x(SL(2,R)/SO(2)) and study explicitly the attractor mechanism for various configurations of extremal black p-branes (antibranes) with the typical near horizon geometries AdS{sub p+2}xS{sup m}xT{sup 6-p-m} and p=0, 1, 2, 3, 4; 2{<=}m{<=}6. Interpretations in terms of wrapped M2 and M5 branes of the 11D M-theory on 3-torus are also given.

  6. 15 CFR 8b.24 - Financial and employment assistance to students.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... students. 8b.24 Section 8b.24 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF... Secondary Education § 8b.24 Financial and employment assistance to students. (a) Provision of financial... person that provides assistance to any of the recipient's students in a manner that discriminates...

  7. 15 CFR 8b.6 - Remedial action, voluntary action, and self-evaluation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... self-evaluation. 8b.6 Section 8b.6 Commerce and Foreign Trade Office of the Secretary of Commerce... DEPARTMENT OF COMMERCE General Provisions § 8b.6 Remedial action, voluntary action, and self-evaluation. (a... or this part and where another recipient exercises control over the recipient that has...

  8. Fast growth associated with aberrant vasculature and hypoxia in fibroblast growth factor 8b (FGF8b) over-expressing PC-3 prostate tumour xenografts

    PubMed Central

    2010-01-01

    Background Prostate tumours are commonly poorly oxygenated which is associated with tumour progression and development of resistance to chemotherapeutic drugs and radiotherapy. Fibroblast growth factor 8b (FGF8b) is a mitogenic and angiogenic factor, which is expressed at an increased level in human prostate tumours and is associated with a poor prognosis. We studied the effect of FGF8b on tumour oxygenation and growth parameters in xenografts in comparison with vascular endothelial growth factor (VEGF)-expressing xenografts, representing another fast growing and angiogenic tumour model. Methods Subcutaneous tumours of PC-3 cells transfected with FGF8b, VEGF or empty (mock) vectors were produced and studied for vascularity, cell proliferation, glucose metabolism and oxygenation. Tumours were evaluated by immunohistochemistry (IHC), flow cytometry, use of radiolabelled markers of energy metabolism ([18F]FDG) and hypoxia ([18F]EF5), and intratumoral polarographic measurements of pO2. Results Both FGF8b and VEGF tumours grew rapidly in nude mice and showed highly vascularised morphology. Perfusion studies, pO2 measurements, [18F]EF5 and [18F]FDG uptake as well as IHC staining for glucose transport protein (GLUT1) and hypoxia inducible factor (HIF) 1 showed that VEGF xenografts were well-perfused and oxygenised, as expected, whereas FGF8b tumours were as hypoxic as mock tumours. These results suggest that FGF8b-induced tumour capillaries are defective. Nevertheless, the growth rate of hypoxic FGF8b tumours was highly increased, as that of well-oxygenised VEGF tumours, when compared with hypoxic mock tumour controls. Conclusion FGF8b is able to induce fast growth in strongly hypoxic tumour microenvironment whereas VEGF-stimulated growth advantage is associated with improved perfusion and oxygenation of prostate tumour xenografts. PMID:21034500

  9. Molecular cloning and characterisation of Ts8B1, Ts8B2 and Ts8B3, three new members of the Taenia solium metacestode 8 kDa diagnostic antigen family.

    PubMed

    Ferrer, Elizabeth; Bonay, Pedro; Foster-Cuevas, Mildred; González, Luis Miguel; Dávila, Iris; Cortéz, María Milagros; Harrison, Leslie J S; Parkhouse, R Michael E; Gárate, Teresa

    2007-03-01

    Antibody screening of a lambdaZAP-XR Taenia solium metacestode cDNA library yielded a clone (Ts8B1), with an insert of 345 bp, and an open reading frame of 258 bp, that coded for a protein with 85 amino acid residues. Alignment of the predicted amino acid sequence with sequences from SWISSPROT revealed an 88% identity with TcA5.5, a 10 kDa immunodiagnostic antigen of T. crassiceps, 75% identity with CyDA a T. solium metacestode antigen, 40-50% identity with several variants of the 8 kDa subunit of antigen B of Echinococcus spp. and with members of the T. solium metacestode 8 kDa antigen family. Two other Ts8B1 related molecules, Ts8B2 and Ts8B3, were identified in the metacestode cDNA library by PCR, coding for 85 and 66 amino acid polypeptides, respectively. Both Ts8B1 and Ts8B2 were characterized as E/S antigens through their subcellular localisation in the secretory membrane system when expressed in NRK cells. The three cDNA inserts were expressed, purified and probed in enzyme-linked immunosorbent assays (ELISA) with sera and cerebro-spinal fluid from patients with confirmed neurocysticercosis, and with sera from pigs infected with T. solium. The most promising antigen, Ts8B2, performed with a sensitivity of 96.8% and specificity of 93.1% in the detection of active NCC when using serum samples in the assay and performed similarly in the porcine system. The implications of these findings are discussed.

  10. F-8C digital CCV flight control laws

    NASA Technical Reports Server (NTRS)

    Hartmann, G. L.; Hauge, J. A.; Hendrick, R. C.

    1976-01-01

    A set of digital flight control laws were designed for the NASA F-8C digital fly-by-wire aircraft. The control laws emphasize Control Configured Vehicle (CCV) benefits. Specific pitch axis objectives were improved handling qualities, angle-of-attack limiting, gust alleviation, drag reduction in steady and maneuvering flight, and a capability to fly with reduced static stability. The lateral-directional design objectives were improved Dutch roll damping and turn coordination over a wide range in angle-of-attack. An overall program objective was to explore the use of modern control design methodilogy to achieve these specific CCV benefits. Tests for verifying system integrity, an experimental design for handling qualities evaluation, and recommended flight test investigations were specified.

  11. Preparation of Al8B4C7 composite materials by using oxide raw materials

    NASA Astrophysics Data System (ADS)

    Zhu, H. X.; Pan, C.; Deng, C. J.; Yuan, W. J.

    2011-10-01

    Al8B4C7 composites materials were prepared by using Al, B2O3 and C as raw materials. The effect of sintering temperature and different additives (Al and C) on Al8B4C7 composites materials were investigated. The Al8B4C7 composites materials were characterized from microstructure, apparent porosity, bulk density and compressive strength. The results demonstrated that the increasing of sintering temperatures could make the samples denser and improve compressive strength. The optimal sintering temperature was 1700 °C, and the main phase composition of Al8B4C7 composites materials were Al8B4C7 and Al2O3. Al additive could improve the properties while C additive played an harmful role. The Al8B4C7 grains were irregular flake and the size was 2~4 μm.

  12. Global gene profiling of aging lungs in Atp8b1 mutant mice

    PubMed Central

    Soundararajan, Ramani; Stearns, Timothy M.; Czachor, Alexander; Fukumoto, Jutaro; Turn, Christina; Westermann-Clark, Emma; Breitzig, Mason; Tan, Lee; Lockey, Richard F.; King, Benjamin L.; Kolliputi, Narasaiah

    2016-01-01

    Objective Recent studies implicate cardiolipin oxidation in several age-related diseases. Atp8b1 encoding Type 4 P-type ATPases is a cardiolipin transporter. Mutation in Atp8b1 gene or inflammation of the lungs impairs the capacity of Atp8b1 to clear cardiolipin from lung fluid. However, the link between Atp8b1 mutation and age-related gene alteration is unknown. Therefore, we investigated how Atp8b1 mutation alters age-related genes. Methods We performed Affymetrix gene profiling of lungs isolated from young (7-9 wks, n=6) and aged (14 months, 14 M, n=6) C57BL/6 and Atp8b1 mutant mice. In addition, Ingenuity Pathway Analysis (IPA) was performed. Differentially expressed genes were validated by quantitative real-time PCR (qRT-PCR). Results Global transcriptome analysis revealed 532 differentially expressed genes in Atp8b1 lungs, 157 differentially expressed genes in C57BL/6 lungs, and 37 overlapping genes. IPA of age-related genes in Atp8b1 lungs showed enrichment of Xenobiotic metabolism and Nrf2-mediated signaling pathways. The increase in Adamts2 and Mmp13 transcripts in aged Atp8b1 lungs was validated by qRT-PCR. Similarly, the decrease in Col1a1 and increase in Cxcr6 transcripts was confirmed in both Atp8b1 mutant and C57BL/6 lungs. Conclusion Based on transcriptome profiling, our study indicates that Atp8b1 mutant mice may be susceptible to age-related lung diseases. PMID:27689529

  13. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good...) Registration statements and reports shall be in the English language. If any exhibit or other paper or...

  14. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good... and reports shall be in the English language. If any exhibit or other paper or document filed with...

  15. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good...) Registration statements and reports shall be in the English language. If any exhibit or other paper or...

  16. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good... and reports shall be in the English language. If any exhibit or other paper or document filed with...

  17. 15 CFR 8b.7 - Designation of responsible employee and adoption of grievance procedures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... and adoption of grievance procedures. 8b.7 Section 8b.7 Commerce and Foreign Trade Office of the... adoption of grievance procedures. (a) Designation of responsible employee. A recipient, other than a small...) Adoption of grievance procedures. A recipient, other than a small recipient, shall adopt...

  18. 15 CFR 8b.7 - Designation of responsible employee and adoption of grievance procedures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... and adoption of grievance procedures. 8b.7 Section 8b.7 Commerce and Foreign Trade Office of the... adoption of grievance procedures. (a) Designation of responsible employee. A recipient, other than a small...) Adoption of grievance procedures. A recipient, other than a small recipient, shall adopt...

  19. The YAV-8B simulation and modeling. Volume 2: Program listing

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Detailed mathematical models of varying complexity representative of the YAV-8B aircraft are defined and documented. These models are used in parameter estimation and in linear analysis computer programs while investigating YAV-8B aircraft handling qualities. Both a six degree of freedom nonlinear model and a linearized three degree of freedom longitudinal and lateral directional model were developed. The nonlinear model is based on the mathematical model used on the MCAIR YAV-8B manned flight simulator. This simulator model has undergone periodic updating based on the results of approximately 360 YAV-8B flights and 8000 hours of wind tunnel testing. Qualified YAV-8B flight test pilots have commented that the handling qualities characteristics of the simulator are quite representative of the real aircraft. These comments are validated herein by comparing data from both static and dynamic flight test maneuvers to the same obtained using the nonlinear program.

  20. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes

    PubMed Central

    Marwaha, Rituraj; Arya, Subhash B.; Jagga, Divya; Kaur, Harmeet

    2017-01-01

    Endocytic, autophagic, and phagocytic vesicles move on microtubule tracks to fuse with lysosomes. Small GTPases, such as Rab7 and Arl8b, recruit their downstream effectors to mediate this transport and fusion. However, the potential cross talk between these two GTPases is unclear. Here, we show that the Rab7 effector PLEKHM1 simultaneously binds Rab7 and Arl8b, bringing about clustering and fusion of late endosomes and lysosomes. We show that the N-terminal RUN domain of PLEKHM1 is necessary and sufficient for interaction with Arl8b and its subsequent localization to lysosomes. Notably, we also demonstrate that Arl8b mediates recruitment of HOPS complex to PLEKHM1-positive vesicle contact sites. Consequently, Arl8b binding to PLEKHM1 is required for its function in delivery and, therefore, degradation of endocytic and autophagic cargo in lysosomes. Finally, we also show that PLEKHM1 competes with SKIP for Arl8b binding, which dictates lysosome positioning. These findings suggest that Arl8b, along with its effectors, orchestrates lysosomal transport and fusion. PMID:28325809

  1. The small GTPase Arl8b regulates assembly of the mammalian HOPS complex on lysosomes

    PubMed Central

    Khatter, Divya; Raina, Vivek B.; Dwivedi, Devashish; Sindhwani, Aastha; Bahl, Surbhi; Sharma, Mahak

    2015-01-01

    The homotypic fusion and protein sorting (HOPS) complex is a multi-subunit complex conserved from yeast to mammals that regulates late endosome and lysosome fusion. However, little is known about how the HOPS complex is recruited to lysosomes in mammalian cells. Here, we report that the small GTPase Arl8b, but not Rab7 (also known as RAB7A), is essential for membrane localization of the human (h)Vps41 subunit of the HOPS complex. Assembly of the core HOPS subunits to Arl8b- and hVps41-positive lysosomes is guided by their subunit–subunit interactions. RNA interference (RNAi)-mediated depletion of hVps41 resulted in the impaired degradation of EGFR that was rescued upon expression of wild-type but not an Arl8b-binding-defective mutant of hVps41, suggesting that Arl8b-dependent lysosomal localization of hVps41 is required for its endocytic function. Furthermore, we have also identified that the Arl8b effector SKIP (also known as PLEKHM2) interacts with and recruits HOPS subunits to Arl8b and kinesin-positive peripheral lysosomes. Accordingly, RNAi-mediated depletion of SKIP impaired lysosomal trafficking and degradation of EGFR. These findings reveal that Arl8b regulates the association of the human HOPS complex with lysosomal membranes, which is crucial for the function of this tethering complex in endocytic degradation. PMID:25908847

  2. TRIP8b Is Required for Maximal Expression of HCN1 in the Mouse Retina

    PubMed Central

    Pan, Yuan; Bhattarai, Sajag; Modestou, Modestos; Drack, Arlene V.; Chetkovich, Dane M.; Baker, Sheila A.

    2014-01-01

    Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are cation-selective channels present in retina, brain and heart. The activity of HCN channels contributes to signal integration, cell excitability and pacemaker activity. HCN1 channels expressed in photoreceptors participate in keeping light responses transient and are required for normal mesopic vision. The subcellular localization of HCN1 varies among cell types. In photoreceptors HCN1 is concentrated in the inner segments while in other retinal neurons, HCN1 is evenly distributed though the cell. This is in contrast to hippocampal neurons where HCN1 is concentrated in a subset of dendrites. A key regulator of HCN1 trafficking and activity is tetratricopeptide repeat-containing Rab8b interacting protein (TRIP8b). Multiple splice isoforms of TRIP8b are expressed throughout the brain and can differentially regulate the surface expression and activity of HCN1. The purpose of the present study was to determine which isoforms of TRIP8b are expressed in the retina and to test if loss of TRIP8b alters HCN1 expression or trafficking. We found that TRIP8b colocalizes with HCN1 in multiple retina neurons and all major splice isoforms of TRIP8b are expressed in the retina. Photoreceptors express three different isoforms. In TRIP8b knockout mice, the ability of HCN1 to traffic to the surface of retinal neurons is unaffected. However, there is a large decrease in the total amount of HCN1. We conclude that TRIP8b in the retina is needed to achieve maximal expression of HCN1. PMID:24409334

  3. Results of acoustic testing of the JT8D-109 refan engines

    NASA Technical Reports Server (NTRS)

    Burdsall, E. A.; Brochu, F. P.; Scaramella, V. M.

    1975-01-01

    A JT8D engine was modified to reduce jet noise levels by 6-8 PNdB at takeoff power without increasing fan generated noise levels. Designated the JT8D-109, the modified engines featured a larger single stage fan, and acoustic treatment in the fan discharge ducts. Noise levels were measured on an outdoor test facility for eight engine/acoustic treatment configurations. Compared to the baseline JT8D, the fully treated JT8D-109 showed reductions of 6 PNdB at takeoff, and 11 PNdB at a typical approach power setting.

  4. Analysis of Coulomb breakup experiments of {sup 8}B with a dynamical eikonal approximation

    SciTech Connect

    Goldstein, G.; Capel, P.; Baye, D.

    2007-08-15

    Various measurements of the Coulomb breakup of {sup 8}B are analyzed within the dynamical eikonal approximation using a single description of {sup 8}B. We obtain a good agreement with experiment for different observables measured between 40 and 80 MeV/nucleon. A simple {sup 7}Be-p potential model description of {sup 8}B seems sufficient to describe all observables. In particular, the asymmetry in parallel-momentum distributions due to E1-E2 interferences is well reproduced without any scaling. The projectile-target nuclear interactions seem negligible if data are selected at forward angles. On the contrary, like in previous analyses we observe a significant influence of higher-order effects. The accuracy of astrophysical S factors for the {sup 7}Be(p,{gamma}){sup 8}B reaction at stellar energies extracted from breakup measurements therefore seems difficult to evaluate.

  5. 30. Photocopy of photograph (from Buffalo Illustrated file #129B8B92, prints ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. Photocopy of photograph (from Buffalo Illustrated file #129B8B92, prints inpossession of Ciminilli Construction,Buffalo, N.Y.), photographer unknown,1890 GENERAL VIEW LOOKING WEST - Cyclorama Building, 369 Franklin Street, Buffalo, Erie County, NY

  6. Structural mechanism for the regulation of HCN ion channels by the accessory protein TRIP8b

    PubMed Central

    DeBerg, Hannah A.; Bankston, John R.; Rosenbaum, Joel C.; Brzovic, Peter S.; Zagotta, William N.; Stoll, Stefan

    2015-01-01

    Summary Hyperpolarization-activated cyclic nucleotide-gated (HCN) ion channels underlie the cationic Ih current present in many neurons. The direct binding of cAMP to HCN channels increases the rate and extent of channel opening and results in a depolarizing shift in the voltage dependence of activation. TRIP8b is an accessory protein that regulates the cell surface expression and dendritic localization of HCN channels and reduces the cyclic nucleotide dependence of these channels. Here we use electron paramagnetic resonance (EPR) to show that TRIP8b binds to the apo state of the cyclic nucleotide-binding domain (CNBD) of HCN2 channels without changing the overall domain structure. With EPR and nuclear magnetic resonance (NMR), we locate TRIP8b relative to the HCN channel and identify the binding interface on the CNBD. These data provide a structural framework for understanding how TRIP8b regulates the cyclic nucleotide dependence of HCN channels. PMID:25800552

  7. VIEW OF ROOMS 8B AND 21B, LOOKING TOWARDS SIDE 3 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    VIEW OF ROOMS 8B AND 21B, LOOKING TOWARDS SIDE 3 OF THE MLP - Cape Canaveral Air Force Station, Launch Complex 39, Mobile Launcher Platforms, Launcher Road, East of Kennedy Parkway North, Cape Canaveral, Brevard County, FL

  8. Full genomic analyses of human rotavirus strains possessing the rare P[8]b VP4 subtype.

    PubMed

    Ghosh, Souvik; Paul, Shyamal Kumar; Yamamoto, Dai; Nagashima, Shigeo; Kobayashi, Nobumichi

    2011-08-01

    Rotaviruses with the P[8] VP4 genotype are a major cause of acute infantile diarrhea. The P[8] genotype is classified into two genetically distinct subtypes, P[8]a and P[8]b. Most of the P[8] strains belong to subtype P[8]a, whilst P[8]b strains are rare. To date, the whole genomes of a few P[8]a strains have been analyzed, whilst there are no reports on full genomic analysis of the P[8]b strains. To determine the genetic makeup of the rare P[8]b strains and their overall genetic relatedness to the P[8]a strains, the present study analyzed the full genomes of a human G9P[8]b strain, MMC38, and a G1P[8]b strain, MMC71, detected in Bangladesh in 2005. By nucleotide sequence identities and phylogenetic analyses, strains MMC38 and MMC71 exhibited a human rotavirus Wa-like genotype constellation. Except for the VP4 gene, all the genes of strains MMC38 and MMC71 were closely related to cognate genes of the contemporary and more recent human Wa-like G1P[8]a, G9P[8]a, G11P[8]a, G11P[25], G12P[6] and G12P[8]a strains, including those from Bangladesh. Therefore, strains MMC38 and MMC71 possessed the genetically distinct P[8]b VP4 gene on a common human Wa-like genetic backbone, pointing towards their possible origin from reassortment events between common human Wa-like strains and unidentified rotavirus strains possessing the rare P[8]b-like VP4 gene. Since strains with this stable Wa-like genetic backbone can spread rapidly, and it is not certain as to whether the current rotavirus vaccines will be equally efficacious against the P[8]b strains as the P[8]a strains, proper detection of P[8]b strains and their whole genomic analyses might be of public health significance. To our knowledge, the present study is the first report on full genomic analysis of the rare P[8]b rotavirus strains.

  9. First-Principles Study of the Electronic Structure and Bonding Properties of X8C46 and X8B6C40 (X: Li, Na, Mg, Ca) Carbon Clathrates

    NASA Astrophysics Data System (ADS)

    KoleŻyński, Andrzej; Szczypka, Wojciech

    2016-03-01

    Results from theoretical analysis of the crystal structure, electronic structure, and bonding properties of C46 and B6C40 carbon clathrates doped with selected alkali and alkaline earth metals cations (Li, Na, Mg, Ca) are presented. The ab initio calculations were performed by means of the WIEN2k package (full potential linearized augmented plane wave method (FP-LAPW) within density functional theory (DFT)) with PBESol and modified Becke-Johnson exchange-correlation potentials used in geometry optimization and electronic structure calculations, respectively. The bonding properties were analyzed by applying Bader's quantum theory of atoms in molecules formalism to the topological properties of total electron density obtained from ab initio calculations. Analysis of the results obtained (i.a. equilibrium geometry, equation of state, cohesive energy, band structure, density of states—both total and projected on to particular atoms, and topological properties of bond critical points and net charges of topological atoms) is presented in detail.

  10. BMP8B Is a Tumor Suppressor Gene Regulated by Histone Acetylation in Gastric Cancer.

    PubMed

    Wisnieski, Fernanda; Leal, Mariana Ferreira; Calcagno, Danielle Queiroz; Santos, Leonardo Caires; Gigek, Carolina Oliveira; Chen, Elizabeth Suchi; Artigiani, Ricardo; Demachki, Sâmia; Assumpção, Paulo Pimentel; Lourenço, Laércio Gomes; Burbano, Rommel Rodríguez; Smith, Marília Cardoso

    2017-04-01

    Different from genetic alterations, the reversible nature of epigenetic modifications provides an interesting opportunity for the development of clinically relevant therapeutics in different tumors. In this study, we aimed to screen and validate candidate genes regulated by the epigenetic marker associated with transcriptional activation, histone acetylation, in gastric cancer (GC). We first compared gene expression profile of trichostatin A-treated and control GC cell lines using microarray assay. Among the 55 differentially expressed genes identified in this analysis, we chose the up-regulated genes BMP8B and BAMBI for further analyses, that included mRNA and histone acetylation quantification in paired GC and nontumor tissue samples. BMP8B expression was reduced in GC compared to nontumor samples (P < 0.01). In addition, reduced BMP8B expression was associated with poorly differentiated GC (P = 0.02). No differences or histopathological associations were identified concerning BAMBI expression. Furthermore, acetylated H3K9 and H4K16 levels at BMP8B were increased in GC compared to nontumors (P < 0.05). However, reduced levels of acetylated H3K9 and H4K16 were associated with poorly differentiated GC (P < 0.05). Reduced levels of acetylated H3K9 was also associated with diffuse-type histological GC (P < 0.05). Notably, reduced BMP8B mRNA and acetylated H4K16 levels were positively correlated in poorly differentiated GC (P < 0.05). Our study demonstrated that BMP8B seems to be a tumor suppressor gene regulated by H4K16 acetylation in poorly differentiated GC. Therefore, BMP8B may be a potential target for TSA-based therapies in this GC sample subset. J. Cell. Biochem. 118: 869-877, 2017. © 2016 Wiley Periodicals, Inc.

  11. ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization.

    PubMed

    Bruurs, Lucas J M; Donker, Lisa; Zwakenberg, Susan; Zwartkruis, Fried J; Begthel, Harry; Knisely, A S; Posthuma, George; van de Graaf, Stan F J; Paulusma, Coen C; Bos, Johannes L

    2015-09-28

    During yeast cell polarization localization of the small GTPase, cell division control protein 42 homologue (Cdc42) is clustered to ensure the formation of a single bud. Here we show that the disease-associated flippase ATPase class I type 8b member 1 (ATP8B1) enables Cdc42 clustering during enterocyte polarization. Loss of this regulation results in increased apical membrane size with scattered apical recycling endosomes and permits the formation of more than one apical domain, resembling the singularity defect observed in yeast. Mechanistically, we show that to become apically clustered, Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids provided by ATP8B1. Disturbing this interaction, either by ATP8B1 depletion or by introduction of a Cdc42 mutant defective in lipid binding, increases Cdc42 mobility and results in apical membrane enlargement. Re-establishing Cdc42 clustering, by tethering it to the apical membrane or lowering its diffusion, restores normal apical membrane size in ATP8B1-depleted cells. We therefore conclude that singularity regulation by Cdc42 is conserved between yeast and human and that this regulation is required to maintain healthy tissue architecture.

  12. ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization

    PubMed Central

    Bruurs, Lucas J.M.; Donker, Lisa; Zwakenberg, Susan; Zwartkruis, Fried J.; Begthel, Harry; Knisely, A.S.; Posthuma, George; van de Graaf, Stan F.J.; Paulusma, Coen C.

    2015-01-01

    During yeast cell polarization localization of the small GTPase, cell division control protein 42 homologue (Cdc42) is clustered to ensure the formation of a single bud. Here we show that the disease-associated flippase ATPase class I type 8b member 1 (ATP8B1) enables Cdc42 clustering during enterocyte polarization. Loss of this regulation results in increased apical membrane size with scattered apical recycling endosomes and permits the formation of more than one apical domain, resembling the singularity defect observed in yeast. Mechanistically, we show that to become apically clustered, Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids provided by ATP8B1. Disturbing this interaction, either by ATP8B1 depletion or by introduction of a Cdc42 mutant defective in lipid binding, increases Cdc42 mobility and results in apical membrane enlargement. Re-establishing Cdc42 clustering, by tethering it to the apical membrane or lowering its diffusion, restores normal apical membrane size in ATP8B1-depleted cells. We therefore conclude that singularity regulation by Cdc42 is conserved between yeast and human and that this regulation is required to maintain healthy tissue architecture. PMID:26416959

  13. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... statement of unincorporated management investment companies currently issuing periodic payment plan...-8B-3, registration statement of unincorporated management investment companies currently issuing..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  14. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... statement of unincorporated management investment companies currently issuing periodic payment plan...-8B-3, registration statement of unincorporated management investment companies currently issuing..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  15. YAV-8B reaction control system bleed and control power usage in hover and transition

    NASA Technical Reports Server (NTRS)

    Borchers, Paul F.; Moralez, Ernesto, III; Merrick, Vernon K.; Stortz, Michael W.

    1994-01-01

    Using a calibrated Rolls-Royce Pegasus engine and existing aircraft instrumentation and pressure taps, total and individual nozzle reaction control system (RCS) bleed flow rates have been measured on a YAV-8B Harrier during typical short takeoff, transition, hover, and vertical landing maneuvers. RCS thrust forces were calculated from RCS nozzle total pressure measurements, and control power was determined from the moments produced by these thrusts and the aircraft's moments of inertia. These data document the characteristics of the YAV-8B RCS with its basic stability augmentation system (SAS) engaged. Advanced control system designs for the YAV-8B can be compared to the original SAS based on the total bleed use and the percentage of available bleed used. In addition, the peak and mean values of the bleed and control power data can be used for sizing the reaction controls for a future short takeoff and vertical landing (STOVL) aircraft.

  16. Nuclear Structure of {sup 8}B Studied by Proton Resonance Scatterings on {sup 7}Be

    SciTech Connect

    Yamaguchi, H.; Wakabayashi, Y.; Hayakawa, S.; Amadio, G.; Kubono, S.; Fujikawa, H.; Niikura, M.; Binh, D. N.; Saito, A.; He, J. J.; Teranishi, T.; Kwon, Y. K.; Nishimura, S.; Togano, Y.; Iwasa, N.; Inafuku, K.; Khiem, L. H.

    2008-05-21

    A new measurement of the proton resonance scattering on {sup 7}Be was performed up to the excitation energy of 6.8 MeV using the low-energy RI beam facility CRIB (CNS Radioactive Ion Beam separator) at the Center for Nuclear Study (CNS) of the University of Tokyo. The excitation function of {sup 8}B above 3.5 MeV was successfully measured for the first time, providing important information about the reaction rate of {sup 7}Be(p,{gamma}){sup 8}B, which is the key reaction in the solar {sup 8}B neutrino production. For more intensive experimental studies with RI beams, the development of a cryogenic gas target system is ongoing at CNS. In this paper a preliminary result of the {sup 7}Be experiment and the present status of the development of the target system are presented.

  17. Analysis of Human TAAR8 and Murine Taar8b Mediated Signaling Pathways and Expression Profile

    PubMed Central

    Mühlhaus, Jessica; Dinter, Juliane; Nürnberg, Daniela; Rehders, Maren; Depke, Maren; Golchert, Janine; Homuth, Georg; Yi, Chun-Xia; Morin, Silke; Köhrle, Josef; Brix, Klaudia; Tschöp, Matthias; Kleinau, Gunnar; Biebermann, Heike

    2014-01-01

    The thyroid hormone derivative 3-iodothyronamine (3-T1AM) exerts metabolic effects in vivo that contradict known effects of thyroid hormones. 3-T1AM acts as a trace amine-associated receptor 1 (TAAR1) agonist and activates Gs signaling in vitro. Interestingly, 3-T1AM-meditated in vivo effects persist in Taar1 knockout-mice indicating that further targets of 3-T1AM might exist. Here, we investigated another member of the TAAR family, the only scarcely studied mouse and human trace-amine-associated receptor 8 (Taar8b, TAAR8). By RT-qPCR and locked-nucleic-acid (LNA) in situ hybridization, Taar8b expression in different mouse tissues was analyzed. Functionally, we characterized TAAR8 and Taar8b with regard to cell surface expression and signaling via different G-protein-mediated pathways. Cell surface expression was verified by ELISA, and cAMP accumulation was quantified by AlphaScreen for detection of Gs and/or Gi/o signaling. Activation of G-proteins Gq/11 and G12/13 was analyzed by reporter gene assays. Expression analyses revealed at most marginal Taar8b expression and no gender differences for almost all analyzed tissues. In heart, LNA-in situ hybridization demonstrated the absence of Taar8b expression. We could not identify 3-T1AM as a ligand for TAAR8 and Taar8b, but both receptors were characterized by a basal Gi/o signaling activity, a so far unknown signaling pathway for TAARs. PMID:25391046

  18. 7Be- and 8B-reaction dynamics at Coulomb barrier energies

    NASA Astrophysics Data System (ADS)

    Mazzocco, M.; Boiano, A.; Boiano, C.; La Commara, M.; Manea, C.; Parascandolo, C.; Pierroutsakou, D.; Signorini, C.; Strano, E.; Torresi, D.; Yamaguchi, H.; Kahl, D.; Acosta, L.; Di Meo, P.; Fernandez-Garcia, J. P.; Glodariu, T.; Grebosz, J.; Guglielmetti, A.; Imai, N.; Hirayama, Y.; Ishiyama, H.; Iwasa, N.; Jeong, S. C.; Jia, H. M.; Keeley, N.; Kim, Y. H.; Kimura, S.; Kubono, S.; Lay, J. A.; Lin, C. J.; Marquinez-Duran, G.; Martel, I.; Miyatake, H.; Mukai, M.; Nakao, T.; Nicoletto, M.; Pakou, A.; Rusek, K.; Sakaguchi, Y.; Sánchez-Benítez, A. M.; Sava, T.; Sgouros, O.; Stefanini, C.; Soramel, F.; Soukeras, V.; Stiliaris, E.; Stroe, L.; Teranishi, T.; Toniolo, N.; Wakabayashi, Y.; Watanabe, Y. X.; Yang, L.; Yang, Y. Y.

    2016-05-01

    We investigated the reaction dynamics induced by the Radioactive Ion Beams 7Be and 8B on a 208Pb target at energies around the Coulomb barrier. The two measurements are strongly interconnected, being 7Be (Sα = 1.586 MeV) the loosely bound core of the even more exotic 8B (Sp = 0.1375 MeV) nucleus. Here we summarize the present status of the data analysis for the measurement of the elastic scattering process for both reactions and the preliminary results for the optical model analysis of the collected data.

  19. Molecular characterization of OP354-like P[8] (P[8]b subtype) human rotaviruses A species isolated in Japan.

    PubMed

    Kuzuya, Mitsutaka; Fujii, Ritsushi; Hamano, Masako; Kida, Kouji; Kanadani, Tomohisa; Nishimura, Keiko; Kishimoto, Toshio

    2012-04-01

    OP354-like P[8] (P[8]b subtype) species A rotaviruses (RVAs) were isolated first time in Japan during a RVA survey in Okayama Prefecture between 2006 and 2009. Two of 236 RVA-positive samples were identified as G1P[8]b by reverse transcription polymerase chain reaction. P[8]b strains (RVA/human-wt/JPN/OH1998/2008/G1P[8]b and RVA/human-wt/JPN/OH2024/2008/G1P[8]b) were isolated only in May, 2008 and both patients infected with P[8]b viruses lived in the same city, suggesting that the prevalence of P[8]b RVAs is limited considerably in Okayama Prefecture. Molecular analysis of four genes (VP4, VP6, VP7, and NSP4 genes) of Japanese P[8]b strains revealed that the VP4 genes of these strains were related closely to those of Southeast Asian and Indian P[8]b strains. In contrast, the VP6, VP7, and NSP4 genes of Japanese P[8]b strains were highly homologous to G1P[8]a strains prevalent in the same area. These results suggest that the Japanese P[8]b strain may be a result of reassortment events between Japanese G1P[8]a viruses and unidentified Asian viruses possessing the P[8]b VP4 gene.

  20. Section 8(d) Health Safety Data Reporting User Guide – Primary Support

    EPA Pesticide Factsheets

    This document presents the user guide for the Office of Pollution Prevention and Toxics (OPPT) Section 8(d) Health & Safety Data Reporting application. This document is the user guide for the Primary Support user.

  1. The expansion of Phytophthora clade 8b: three new species associated with winter grown vegetable crops.

    PubMed

    Bertier, L; Brouwer, H; de Cock, A W A M; Cooke, D E L; Olsson, C H B; Höfte, M

    2013-12-01

    Despite its association with important agricultural crops, Phytophthora clade 8b is a poorly studied group of species. The clade currently consists of three officially described species (Phytophthora porri, P. brassicae and P. primulae) that are host-specific pathogens of leek, cabbages and Primula spp., respectively. However, over the past few decades, several other clade 8b-like Phytophthoras have been found on a variety of different host plants that were all grown at low temperatures in winter seasons. In this study, a collection of 30 of these isolates was subjected to a phylogenetic study using two loci (the rDNA ITS region and the mitochondrial cox1 gene). This analysis revealed a clear clustering of isolates according to their host plants. To verify whether these isolates belong to separate species, a detailed morphological study was conducted. On the basis of genetic and morphological differences and host specificity, we now present the official description of three new species in clade 8b: Phytophthora cichorii sp. nov., P. dauci sp. nov. and P. lactucae sp. nov. Two other groups of isolates (Phytophthora taxon castitis and Phytophthora taxon parsley) might also represent new species but the data available at this time are insufficient for an official description. This brings Phytophthora clade 8b to a group of six species that are all host-specific, slow-growing and specifically infect herbaceous crops at low temperatures.

  2. Program for refan JT8D engine design, fabrication and test, phase 2

    NASA Technical Reports Server (NTRS)

    Glass, J. A.; Zimmerman, E. S.; Scaramella, V. M.

    1975-01-01

    The objective of the JT8D refan program was to design, fabricate, and test certifiable modifications of the JT8D engine which would reduce noise generated by JT8D powered aircraft. This was to be accomplished without affecting reliability and maintainability, at minimum retrofit cost, and with no performance penalty. The mechanical design, engine performance and stability characteristics at sea-level and altitude, and the engine noise characteristics of the test engines are documented. Results confirmed the structural integrity of the JT8D-109. Engine operation was stable throughout the airplane flight envelope. Fuel consumption of the test engines was higher than that required to meet the goal of no airplane performance penalty, but the causes were identified and corrected during a normal pre-certification engine development program. Compared to the baseline JT8D-109 engine, the acoustically treated JT8D-109 engine showed noise reductions of 6 PNdB at takeoff and 11 PNdB at a typical approach power setting.

  3. CF8C-Plus: A New High Temperature Austenitic Casting for Advanced Power Systems

    SciTech Connect

    Shingledecker, John P; Maziasz, Philip J; Evans, Neal D; Santella, Michael L

    2006-01-01

    A new cast austenitic stainless steel, CF8C plus, has been developed by Oak Ridge National Laboratory and Caterpillar for a wide range of transportation and energy applications. CF8C plus steel has improved high temperature tensile, creep, fatigue, and creep-fatigue properties compared with standard CF8C steel. Changes to the CF8C steel composition, including additions of Mn and N, result in changes to the solidification behaviour and final microstructure of the alloy, which directly relate to the improved mechanical properties. Additionally, CF8C plus is a relatively inexpensive steel which exhibits good castability. The mechanical properties of the alloy have generated significant interest for the production/design of cast components for diesel engine turbochargers and other exhaust components, natural gas reciprocating engines for distributed power, and turbine end covers and casings for land based turbines. In the present paper, the microstructural evolution of CF8C and CF8C plus are presented in more detail, and the mechanical properties of the alloys are compared with each other and other engineering alloys.

  4. Determination of YAV-8B Reaction Control System bleed flow usage

    NASA Technical Reports Server (NTRS)

    Borchers, Paul F.; Moralez, Ernesto, III; Merrick, Vernon K.; Stortz, Michael W.; Eames, David J. H.

    1992-01-01

    Using a calibrated Rolls-Royce Pegasus engine, total Reaction Control System (RCS) bleed flow rates have been measured on a YAV-8B Harrier during typical short takeoff, transition, hover and vertical landing maneuvers. Using existing aircraft instrumentation and pressure taps located in the RCS ducts, bleed flow rates at each RCS valve were also measured directly during flight and ground tests. These data were compared with the calibrated engine data and with the RCS part of a YAV-8B mathematical model used in piloted simulation at NASA Ames Research Center. Areas of disagreement were small, being confined to the estimation of closed RCS valve leakages and the modeling of the RCS butterfly valve pressure losses.

  5. Measurement of the 8B Solar Neutrino Flux with KamLAND

    SciTech Connect

    Abe, S.; Furuno, K.; Gando, A.; Gando, Y.; Ichimura, K.; Ikeda, H.; Inoue, K.; Kibe, Y.; Kimura, W.; Kishimoto, Y.; Koga, M.; Minekawa, Y.; Mitsui, T.; Morikawa, T.; Nagai, N.; Nakajima, K.; Nakamura, K.; Nakamura, M.; Narita, K.; Shimizu, I.; Shimizu, Y.; Shirai, J.; Suekane, F.; Suzuki, A.; Takahashi, H.; Takahashi, N.; Takemoto, Y.; Tamae, K.; Watanabe, H.; Xu, B.D.; Yabumoto, H.; Yonezawa, E.; Yoshida, H.; Yoshida, S.; Enomoto, S.; Kozlov, A.; Murayama, H.; Grant, C.; Keefer, G.; McKee, D.; Piepke, A.; Banks, T.I.; Bloxham, T.; Detwiler, J.A.; Freedman, S.J.; Fujikawa, B.K.; Han, K.; Kadel, R.; O'Donnell, T.; Steiner, H.M.; Winslow, L.A.; Dwyer, D.A.; Mauger, C.; McKeown, R.D.; Zhang, C.; Berger, B.E.; Lane, C.E.; Maricic, J.; Miletic, T.; Batygov, M.; Learned, J.G.; Matsuno, S.; Pakvasa, S.; Sakai, M.; Horton-Smith, G.A.; Tang, A.; Downum, K.E.; Gratta, G.; Tolich, K.; Efremenko, Y.; Kamyshkov, Y.; Perevozchikov, O.; Karwowski, H.J.; Markoff, D.M.; Tornow, W.; Heeger, K.M.; Piquemal, F.; Ricol, J.-S.; Decowski, M.P.

    2011-06-04

    We report a measurement of the neutrino-electron elastic scattering rate from {sup 8}B solar neutrinos based on a 123 kton-day exposure of KamLAND. The background-subtracted electron recoil rate, above a 5.5-MeV analysis threshold is 1.49 {+-} 0.14(stat) {+-} 0.17(syst) events per kton-day. Interpreted as due to a pure electron flavor flux with a {sup 8}B neutrino spectrum, this corresponds to a spectrum integrated flux of 2.77 {+-} 0.26(stat) {+-} 0.32(syst) x 10{sup 6} cm{sup -2}s{sup -1}. The analysis threshold is driven by {sup 208}Tl present in the liquid scintillator, and the main source of systematic uncertainty is due to background from cosmogenic {sup 11}Be. The measured rate is consistent with existing measurements and with standard solar model predictions which include matter-enhanced neutrino oscillation.

  6. A lower radius and mass for the transiting extrasolar planet HAT-P-8 b

    NASA Astrophysics Data System (ADS)

    Mancini, L.; Southworth, J.; Ciceri, S.; Fortney, J. J.; Morley, C. V.; Dittmann, J. A.; Tregloan-Reed, J.; Bruni, I.; Barbieri, M.; Evans, D. F.; D'Ago, G.; Nikolov, N.; Henning, Th.

    2013-03-01

    Context. The extrasolar planet HAT-P-8 b was thought to be one of the more inflated transiting hot Jupiters. Aims: By using new and existing photometric data, we computed precise estimates of the physical properties of the system. Methods: We present photometric observations comprising eleven light curves covering six transit events, obtained using five medium-class telescopes and telescope-defocussing technique. One transit was simultaneously obtained through four optical filters, and two transits were followed contemporaneously from two observatories. We modelled these and seven published datasets using the jktebop code. The physical parameters of the system were obtained from these results and from published spectroscopic measurements. In addition, we investigated the theoretically-predicted variation of the apparent planetary radius as a function of wavelength, covering the range 330-960 nm. Results: We find that HAT-P-8 b has a significantly lower radius (1.321 ± 0.037 RJup) and mass (1.275 ± 0.053 MJup) compared to previous estimates (1.50-0.06+0.08 R_{Jup} and 1.52-0.16+0.18 M_{Jup} respectively). We also detect a radius variation in the optical bands that, when compared with synthetic spectra of the planet, may indicate the presence of a strong optical absorber, perhaps TiO and VO gases, near the terminator of HAT-P-8 b. Conclusions: These new results imply that HAT-P-8 b is not significantly inflated, and that its position in the planetary mass-radius diagram is congruent with those of many other transiting extrasolar planets. Full Table 2 is only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/551/A11

  7. Complete Genome Sequence of Fowl Aviadenovirus Serotype 8b Isolated in South America.

    PubMed

    Izquierdo-Lara, Ray; Calderón, Katherine; Chumbe, Ana; Montesinos, Ricardo; Montalván, Ángela; González, Armando E; Icochea, Eliana; Fernández-Díaz, Manolo

    2016-10-20

    We present here the complete genome sequence of fowl aviadenovirus E (FAdV-E) serotype 8b strain FV211-16, isolated from chickens with inclusion body hepatitis in Peru. Genome comparisons with other FAdV-E strains revealed identities of 84.9 to 97.1% and the presence of 9 and 2 unique amino acid mutations in hexon and fiber proteins, respectively.

  8. Complete Genome Sequence of Fowl Aviadenovirus Serotype 8b Isolated in South America

    PubMed Central

    Calderón, Katherine; Chumbe, Ana; Montesinos, Ricardo; Montalván, Ángela; González, Armando E.; Icochea, Eliana; Fernández-Díaz, Manolo

    2016-01-01

    We present here the complete genome sequence of fowl aviadenovirus E (FAdV-E) serotype 8b strain FV211-16, isolated from chickens with inclusion body hepatitis in Peru. Genome comparisons with other FAdV-E strains revealed identities of 84.9 to 97.1% and the presence of 9 and 2 unique amino acid mutations in hexon and fiber proteins, respectively. PMID:27795284

  9. Characterization of mutations in ATP8B1 associated with hereditary cholestasis.

    PubMed

    Klomp, Leo W J; Vargas, Julie C; van Mil, Saskia W C; Pawlikowska, Ludmila; Strautnieks, Sandra S; van Eijk, Michiel J T; Juijn, Jenneke A; Pabón-Peña, Carlos; Smith, Lauren B; DeYoung, Joseph A; Byrne, Jane A; Gombert, Justijn; van der Brugge, Gerda; Berger, Ruud; Jankowska, Irena; Pawlowska, Joanna; Villa, Erica; Knisely, A S; Thompson, Richard J; Freimer, Nelson B; Houwen, Roderick H J; Bull, Laura N

    2004-07-01

    Progressive familial intrahepatic cholestasis (PFIC) and benign recurrent intrahepatic cholestasis (BRIC) are clinically distinct hereditary disorders. PFIC patients suffer from chronic cholestasis and develop liver fibrosis. BRIC patients experience intermittent attacks of cholestasis that resolve spontaneously. Mutations in ATP8B1 (previously FIC1) may result in PFIC or BRIC. We report the genomic organization of ATP8B1 and mutation analyses of 180 families with PFIC or BRIC that identified 54 distinct disease mutations, including 10 mutations predicted to disrupt splicing, 6 nonsense mutations, 11 small insertion or deletion mutations predicted to induce frameshifts, 1 large genomic deletion, 2 small inframe deletions, and 24 missense mutations. Most mutations are rare, occurring in 1-3 families, or are limited to specific populations. Many patients are compound heterozygous for 2 mutations. Mutation type or location correlates overall with clinical severity: missense mutations are more common in BRIC (58% vs. 38% in PFIC), while nonsense, frameshifting, and large deletion mutations are more common in PFIC (41% vs. 16% in BRIC). Some mutations, however, lead to a wide range of phenotypes, from PFIC to BRIC or even no clinical disease. ATP8B1 mutations were detected in 30% and 41%, respectively, of the PFIC and BRIC patients screened.

  10. Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes.

    PubMed

    Kobayashi, Shintaro; Suzuki, Tadaki; Kawaguchi, Akira; Phongphaew, Wallaya; Yoshii, Kentaro; Iwano, Tomohiko; Harada, Akihiro; Kariwa, Hiroaki; Orba, Yasuko; Sawa, Hirofumi

    2016-03-18

    West Nile virus (WNV) particles assemble at and bud into the endoplasmic reticulum (ER) and are secreted from infected cells through the secretory pathway. However, the host factor related to these steps is not fully understood. Rab proteins, belonging to the Ras superfamily, play essential roles in regulating many aspects of vesicular trafficking. In this study, we sought to determine which Rab proteins are involved in intracellular trafficking of nascent WNV particles. RNAi analysis revealed that Rab8b plays a role in WNV particle release. We found that Rab8 and WNV antigen were colocalized in WNV-infected human neuroblastoma cells, and that WNV infection enhanced Rab8 expression in the cells. In addition, the amount of WNV particles in the supernatant of Rab8b-deficient cells was significantly decreased compared with that of wild-type cells. We also demonstrated that WNV particles accumulated in the recycling endosomes in WNV-infected cells. In summary, these results suggest that Rab8b is involved in trafficking of WNV particles from recycling endosomes to the plasma membrane.

  11. Evolution of substellar 'brown' dwarfs and the evolutionary status of VB8B

    NASA Technical Reports Server (NTRS)

    Stringfellow, Guy S.

    1986-01-01

    Detailed evolutionary calculations for stars near and below the minimum main sequence (MS) mass are presented, and the results are compared to VB8B. The standard set of stellar equations are solved, and the influence of both high and low grain opacities is assessed. All masses less than 0.08 solar are found to evolve to 0.0001 solar luminosities in a billion years or less. Both 0.08 solar mass and 0.075 solar mass stars become MS stars after five billion years, but with luminosities substantially below 0.0001 solar. The best fit to VB8B is 0.06 + or - 0.02 solar mass, but less than 0.8 solar mass. Depending upon its very precise mass, VB8B can be either a substellar brown dwarf with an age of 0.3-3 billion years, or an MS star with an age anywhere from two billion years to a Hubble time.

  12. Utilization Chitosan-p-t-Butylcalix[4]Arene for Red MX 8B Adsorbent

    NASA Astrophysics Data System (ADS)

    Handayani, D. S.; Kusumaningsih, T.; Hak, L. A.

    2017-02-01

    Adsorption of Procion Red MX 8B using chitosan dan chitosan-linked p-t-butylcalix[4]arene has been done. The research aimed to understand the adsorption of Procion Red MX 8B using chitosan p-t-butylcalix[4]arene compared to ordinary chitosan. The research was conducted in a batch process varying in pH, contact time and initial concentration of the Procion Red MX 8B. The amount of dye adsorbed was determined using UV-Vis spectrophotometer and the adsorben was characterized using FTIR and SEM-EDX spectrophotometer. The result showed that the optimum condition was achieved when the pH was set at 4, contact time 135 minutes and initial concentration at 200 ppm. The kinetic analysis showed that the adsorption followed Ho kinetic model and pseudo second order with the adsorption rate constant was 3.69×10-3 g/mg.minute and 2.03×10-3 g/mg.minute. The isotherm analysis showed that the adsorption process tend to occur following the Langmuir model with maximum capacity for chitosan and chitosan-linked p-t-butylcalix[4]arene 136.09 mg/g and 147.35 mg/g respectively. The adsorption energy of chitosan and chitosan-linked p-t-butylcalix[4]arene at 30.53 kJ/mole and 33.65 kJ/mole.

  13. Pollution reduction technology program for class T4(JT8D) engines

    NASA Technical Reports Server (NTRS)

    Roberts, R.; Fiorentino, A. J.; Diehl, L. A.

    1977-01-01

    The technology required to develop commercial gas turbine engines with reduced exhaust emissions was demonstrated. Can-annular combustor systems for the JT8D engine family (EPA class T4) were investigated. The JT8D turbofan engine is an axial-flow, dual-spool, moderate-bypass-ratio design. It has a two-stage fan, a four-stage low-pressure compressor driven by a three-stage low-pressure turbine, and a seven-stage high-pressure compressor driven by a single-stage high-pressure turbine. A cross section of the JT8D-17 showing the mechanical configuration is given. Key specifications for this engine are listed.

  14. Summary and analysis of performance and stability characteristics of the refan JT8D-109 engine

    NASA Technical Reports Server (NTRS)

    Sanberg, K. G.; Mogielnicki, R. M.; Davis, J. C.; Scaramella, V. M.

    1975-01-01

    The refan JT8D-109 performance and stability characteristics are reported as determined from sea level testing, altitude testing, and DC-9 flight testing. The test results are summarized as follows: (1) TSFC at SLS achieved design goal of 12.66 percent reduction. (2) TSFC at altitude average cruise power 0.5 percent higher than design goal, (3) TSFC at altitude maximum cruise power 1.7-3.7 percent higher than design goal, (4) ground starting consistent with JT8D-9 base engine, (5) successful flight starts without starter assist, (6) transient surge margin equivalent to JT8D-9, (7) stable engine operation with inlet distortion, and (8) stable engine operation during snap acceleration and deceleration. A flight idle setting is required for acceptable aborted-landing go-around acceleration time due to increase in low-rotor moment of inertia, and a performance improvement program should be conducted as part of any future certification program.

  15. HATS-8b: A Low-density Transiting Super-Neptune

    NASA Astrophysics Data System (ADS)

    Bayliss, D.; Hartman, J. D.; Bakos, G. Á.; Penev, K.; Zhou, G.; Brahm, R.; Rabus, M.; Jordán, A.; Mancini, L.; de Val-Borro, M.; Bhatti, W.; Espinoza, N.; Csubry, Z.; Howard, A. W.; Fulton, B. J.; Buchhave, L. A.; Henning, T.; Schmidt, B.; Ciceri, S.; Noyes, R. W.; Isaacson, H.; Marcy, G. W.; Suc, V.; Lázár, J.; Papp, I.; Sári, P.

    2015-08-01

    HATS-8b is a low density transiting super-Neptune discovered as part of the HATSouth project. The planet orbits its solar-like G-dwarf host (V = 14.03+/- 0.10, {T}{eff} = 5679+/- 50 K) with a period of 3.5839 days. HATS-8b is the third lowest-mass transiting exoplanet to be discovered from a wide-field ground-based search, and with a mass of 0.138+/- 0.019 {M}{{J}} it is approximately halfway between the masses of Neptune and Saturn. However, HATS-8b has a radius of {0.873}-0.075+0.123 {R}{{J}}, resulting in a bulk density of just 0.259+/- 0.091 {{g}} {{cm}}-3. The metallicity of the host star is super-solar ([{Fe}/{{H}}] = 0.210+/- 0.080), providing evidence against the idea that low-density exoplanets form from metal-poor environments. The low density and large radius of HATS-8b results in an atmospheric scale height of almost 1000 km, and in addition to this there is an excellent reference star of nearly equal magnitude at just 19″ separation in the sky. These factors make HATS-8b an exciting target for future atmospheric characterization studies, particularly for long-slit transmission spectroscopy. The HATSouth network is operated by a collaboration consisting of Princeton University (PU), the Max Planck Institute für Astronomie (MPIA), the Australian National University (ANU), and the Pontificia Universidad Católica de Chile (PUC). The station at Las Campanas Observatory (LCO) of the Carnegie Institute is operated by PU in conjunction with PUC, the station at the High Energy Spectroscopic Survey site is operated in conjunction with MPIA, and the station at Siding Spring Observatory is operated jointly with ANU. This paper includes data gathered with the 6.5 m Magellan Telescopes located in LCO, Chile. The work is based in part on observations made with the MPG 2.2 m Telescope and the ESO 3.6 m Telescope at the ESO Observatory in La Silla. This paper uses observations obtained using the facilities of the Las Cumbres Observatory Global Telescope.

  16. 13 CFR 121.410 - What are the size standards for SBA's Section 8(d) Subcontracting Program?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... SBA's Section 8(d) Subcontracting Program? 121.410 Section 121.410 Business Credit and Assistance...'s Section 8(d) Subcontracting Program? For subcontracting purposes pursuant to sections 8(d) of the... Aerospace Equipment and Military Weapons under NAICS code 541330....

  17. 13 CFR 121.410 - What are the size standards for SBA's Section 8(d) Subcontracting Program?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... SBA's Section 8(d) Subcontracting Program? 121.410 Section 121.410 Business Credit and Assistance...'s Section 8(d) Subcontracting Program? For subcontracting purposes pursuant to sections 8(d) of the... Aerospace Equipment and Military Weapons under NAICS code 541330....

  18. 13 CFR 121.410 - What are the size standards for SBA's Section 8(d) Subcontracting Program?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... SBA's Section 8(d) Subcontracting Program? 121.410 Section 121.410 Business Credit and Assistance...'s Section 8(d) Subcontracting Program? For subcontracting purposes pursuant to sections 8(d) of the... Aerospace Equipment and Military Weapons under NAICS code 541330....

  19. 13 CFR 121.410 - What are the size standards for SBA's Section 8(d) Subcontracting Program?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... SBA's Section 8(d) Subcontracting Program? 121.410 Section 121.410 Business Credit and Assistance...'s Section 8(d) Subcontracting Program? For subcontracting purposes pursuant to sections 8(d) of the... Aerospace Equipment and Military Weapons under NAICS code 541330....

  20. DC-9 flight demonstration program with refanned JT8D engines. Volume 1: Summary

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The design, analysis, fabrication, and ground and flight testing of DC-9 airframe/nacelle hardware with prototype JT8D-109 engines are discussed. The installation of the JT8D-109 engine on the DC-9 Refan airplane required new or modified hardware for the pylon, nacelle, and fuselage. The acoustic material used in the nose cowl was bonded aluminum honeycomb sandwich and the exhaust duct acoustic material was Inconel 625 Stresskin. The sea level static, standard day bare engine takeoff thrust, the cruise TSFC and the maximum available cruise thrust for the JT8D-109 engine were compared with those of the JT8D-9 engine. The range capabilities of the DC-9 Refan and the production DC-9 airplane were also compared. The Refan airplane demonstrated flight characteristics similar to the production DC-9-30 and satisfied airworthiness requirements. Flyover noise levels were determined for the DC-9 Refan and the DC-9 C-9A airplane for takeoff and landing conditions. Cost estimates were also made.

  1. Nonequilibrium THz Conductivity of Bi2Sr2CaCu2O8+d

    SciTech Connect

    Carnahan, M.A.; Kaindl, R.A.; Orenstein, J.; Chemla, D.S.; Oh,S.; Eckstein, J.N.

    2003-05-27

    Using high sensitivity visible-pump/THz-probe spectroscopywe investigate the dynamics of the complex optical conductivity inoptimally-doped Bi2Sr2CaCu2O8+d films directly after photoexcitation. Thephotoinduced change in the imaginary part, indicative of a reduction inthe superconducting condensate density, saturates at higherlaser-fluences and shows a complete destruction of thecondensate.

  2. DETECTING GRAVITY MODES IN THE SOLAR {sup 8} B NEUTRINO FLUX

    SciTech Connect

    Lopes, Ilídio; Turck-Chièze, Sylvaine E-mail: ilopes@uevora.pt

    2014-09-10

    The detection of gravity modes produced in the solar radiative zone has been a challenge in modern astrophysics for more than 30 yr and their amplitude in the core is not yet determined. In this Letter, we develop a new strategy to look for standing gravity modes through solar neutrino fluxes. We note that due to a resonance effect, the gravity modes of low degree and low order have the largest impact on the {sup 8} B neutrino flux. The strongest effect is expected to occur for the dipole mode with radial order 2, corresponding to periods of about 1.5 hr. These standing gravity waves produce temperature fluctuations that are amplified by a factor of 170 in the boron neutrino flux for the corresponding period, in consonance with the gravity modes. From current neutrino observations, we determine that the maximum temperature variation due to the gravity modes in the Sun's core is smaller than 5.8 × 10{sup –4}. This study clearly shows that due to their high sensitivity to the temperature, the {sup 8} B neutrino flux time series is an excellent tool to determine the properties of gravity modes in the solar core. Moreover, if gravity mode footprints are discovered in the {sup 8} B neutrino flux, this opens a new line of research to probe the physics of the solar core as non-standing gravity waves of higher periods cannot be directly detected by helioseismology but could leave their signature on boron neutrino or on other neutrino fluxes.

  3. Structure of 8B from elastic and inelastic 7Be+p scattering

    NASA Astrophysics Data System (ADS)

    Mitchell, J. P.; Rogachev, G. V.; Johnson, E. D.; Baby, L. T.; Kemper, K. W.; Moro, A. M.; Peplowski, P.; Volya, A. S.; Wiedenhöver, I.

    2013-05-01

    Background: Detailed experimental knowledge of the level structure of light weakly bound nuclei is necessary to guide the development of new theoretical approaches that combine nuclear structure with reaction dynamics.Purpose: The resonant structure of 8B is studied in this work.Method: Excitation functions for elastic and inelastic 7Be+p scattering were measured using a 7Be rare isotope beam. Excitation energies ranging between 1.6 and 3.4 MeV were investigated. An R-matrix analysis of the excitation functions was performed.Results: New low-lying resonances at 1.9, 2.54, and 3.3 MeV in 8B are reported with spin-parity assignment 0+, 2+, and 1+, respectively. Comparison to the time-dependent continuum shell (TDCSM) model and ab initio no-core shell model/resonating-group method (NCSM/RGM) calculations is performed. This work is a more detailed analysis of the data first published as a Rapid Communication. J. P. Mitchell, G. V. Rogachev, E. D. Johnson, L. T. Baby, K. W. Kemper , [Phys. Rev. CPRVCAN0556-281310.1103/PhysRevC.82.011601 82, 011601(R) (2010)].Conclusions: Identification of the 0+, 2+, 1+ states that were predicted by some models at relatively low energy but never observed experimentally is an important step toward understanding the structure of 8B. Their identification was aided by having both elastic and inelastic scattering data. Direct comparison of the cross sections and phase shifts predicted by the TDCSM and ab initio no-core shell model coupled with the resonating group method is of particular interest and provides a good test for these theoretical approaches.

  4. An 8-b 1.3-MHz successive-approximation A/D converter

    NASA Technical Reports Server (NTRS)

    Hadidi, KH.; Tso, Vincent S.; Temes, Gabor C.

    1990-01-01

    A new successive-approximation A/D converter is described. It combines a string of equal-valued polysilicon resistors and a set of ratioed capacitors in a unique circuit configuration so that a high sampling rate is achieved. The comparator is realized by a chopper-stabilized amplifier to reduce the effect of the offset voltages of MOS amplifiers. The converter performs an 8-b monotonic conversion with a differential nonlinearity less than 1 LSB in 770 nsec. The die area is 3750 sq mil. This new conversion technique can also be utilized in a pipelined A/D converter (Temes, 1985) and enables it to achieve high speed.

  5. 15 CFR 8b.10 - Effect of state or local law or other requirements and effect of employment opportunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Effect of state or local law or other requirements and effect of employment opportunities. 8b.10 Section 8b.10 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION AGAINST THE HANDICAPPED IN FEDERALLY ASSISTED PROGRAMS OPERATED BY THE DEPARTMENT...

  6. WASP-8b: CHARACTERIZATION OF A COOL AND ECCENTRIC EXOPLANET WITH SPITZER

    SciTech Connect

    Cubillos, Patricio; Harrington, Joseph; Hardy, Ryan A.; Blecic, Jasmina; Hardin, Matthew; Campo, Christopher J.; Madhusudhan, Nikku; Stevenson, Kevin B.; Anderson, David R.

    2013-05-01

    WASP-8b has 2.18 times Jupiter's mass and is on an eccentric (e = 0.31) 8.16 day orbit. With a time-averaged equilibrium temperature of 948 K, it is one of the least-irradiated hot Jupiters observed with the Spitzer Space Telescope. We have analyzed six photometric light curves of WASP-8b during secondary eclipse observed in the 3.6, 4.5, and 8.0 {mu}m Infrared Array Camera bands. The eclipse depths are 0.113% {+-} 0.018%, 0.069% {+-} 0.007%, and 0.093% {+-} 0.023%, respectively, giving respective brightness temperatures of 1552, 1131, and 938 K. We characterized the atmospheric thermal profile and composition of the planet using a line-by-line radiative transfer code and a Markov-chain Monte Carlo sampler. The data indicated no thermal inversion, independently of any assumption about chemical composition. We noted an anomalously high 3.6 {mu}m brightness temperature (1552 K); by modeling the eccentricity-caused thermal variation, we found that this temperature is plausible for radiative timescales less than {approx}10{sup 2} hr. However, as no model spectra fit all three data points well, the temperature discrepancy remains as an open question.

  7. Adsorption of Procion Red MX 8B using spent tea leaves as adsorbent

    NASA Astrophysics Data System (ADS)

    Heraldy, Eddy; Osa, Riesta Ramdhaniyati; Suryanti, Venty

    2016-02-01

    The adsorption of Procion Red MX 8B using spent tea leaves (STL) as adsorbent, has been studied by batch adsorption technique. The adsorbent was activated by NaOH 4% for 24 hours for delignification process. The adsorbent was characterized using FTIR to indetify the functional groups of cellulose was shown by uptake -OH, C-H and C-O. The optimum conditions of adsorption experiments were achieved when pH was set as 6 with contact time of 75 minutes and capacity of adsorption was 3.28 mg/g. The equilibrium data were fitted to Langmuir and Isotherm Freundlichs. The kinetic models, pseudo first order and pseudo second order were employed to describe the adsorption mechanism. The experimental results showed that the pseudo second order equation was the best model that described the adsorption behavior with the coefficient of correlation (R2) was equal higher than 0.99 The results suggested that STL had high potential to be used as effective adsorbent for Procion Red MX 8B removal.

  8. WASP-8b: Characterization of a Cool and Eccentric Exoplanet with Spitzer

    NASA Astrophysics Data System (ADS)

    Cubillos, Patricio; Harrington, Joseph; Madhusudhan, Nikku; Stevenson, Kevin B.; Hardy, Ryan A.; Blecic, Jasmina; Anderson, David R.; Hardin, Matthew; Campo, Christopher J.

    2013-05-01

    WASP-8b has 2.18 times Jupiter's mass and is on an eccentric (e = 0.31) 8.16 day orbit. With a time-averaged equilibrium temperature of 948 K, it is one of the least-irradiated hot Jupiters observed with the Spitzer Space Telescope. We have analyzed six photometric light curves of WASP-8b during secondary eclipse observed in the 3.6, 4.5, and 8.0 μm Infrared Array Camera bands. The eclipse depths are 0.113% ± 0.018%, 0.069% ± 0.007%, and 0.093% ± 0.023%, respectively, giving respective brightness temperatures of 1552, 1131, and 938 K. We characterized the atmospheric thermal profile and composition of the planet using a line-by-line radiative transfer code and a Markov-chain Monte Carlo sampler. The data indicated no thermal inversion, independently of any assumption about chemical composition. We noted an anomalously high 3.6 μm brightness temperature (1552 K) by modeling the eccentricity-caused thermal variation, we found that this temperature is plausible for radiative timescales less than ~102 hr. However, as no model spectra fit all three data points well, the temperature discrepancy remains as an open question.

  9. Nqrs Data for C10H8D2 (Subst. No. 1222)

    NASA Astrophysics Data System (ADS)

    Chihara, H.; Nakamura, N.

    This document is part of Subvolume A `Substances Containing Ag … C10H15' of Volume 48 `Nuclear Quadrupole Resonance Spectroscopy Data' of Landolt-Börnstein - Group III `Condensed Matter'. It contains an extract of Section `3.2 Data tables' of the Chapter `3 Nuclear quadrupole resonance data' providing the NQRS data for C10H8D2 (Subst. No. 1222)

  10. Cast CF8C-Plus Stainless Steel for Turbocharger Applications

    SciTech Connect

    Maziasz, P.J.; Shyam, A.; Evans, N.D.; Pattabiraman, K. (Honeywell Turbo Technologies

    2010-06-30

    The purpose of this Cooperative Research and Development Agreement (CRADA) project is to provide the critical test data needed to qualify CF8C-Plus cast stainless steel for commercial production and use for turbocharger housings with upgraded performance and durability relative to standard commercial cast irons or stainless steels. The turbocharger technologies include, but are not limited to, heavy-duty highway diesel engines, and passenger vehicle diesel and gasoline engines. This CRADA provides additional critical high-temperature mechanical properties testing and data analysis needed to quality the new CF8C-Plus steels for turbocharger housing applications.

  11. t-C8B2N2: A potential superhard material

    NASA Astrophysics Data System (ADS)

    Wang, Dong; Shi, Rui; Gan, Li-Hua

    2017-02-01

    A potential superhard material C8B2N2 with I-4m2 space group is found and confirmed to be stable with first-principles calculations. The results show that its structure is highly incompressible with bulk modulus of 383.4 GPa and shear modulus of 383.0 GPa. It shows that this material is nearly isotropy with universal anisotropy index of 0.056, and its fractional anisotropy ratio of shear modulus and bulk modulus are 0.0055 and 0.0, respectively. Interestingly, its theoretical bulk modulus, shear modules, Young's modulus, Poisson's ratio and Vickers hardness are almost same to those of well-known superhard material c-BN.

  12. Flight test and numerical simulation of transonic flow around YAV-8B Harrier II wing

    NASA Technical Reports Server (NTRS)

    Gea, Lie-Mine; Chyu, Wei J.; Stortz, Michael W.; Roberts, Andrew C.; Chow, Chuen-Yen

    1991-01-01

    A computational fluid dynamics (CFD) method is used to study the aerodynamics of the YAV-8B Harrier II wing in the transonic region. A numerical procedure is developed to compute the flow field around the complicated wing-pylon-fairing geometry. The surface definition of the wing and pylons were obtained from direct measurement using theodolite triangulation. A thin-layer Navier-Stokes code with the Chimera technique is used to compute flow solutions. The computed pressure distributions at several span stations are compared with flight test data and show good agreement. Computed results are correlated with flight test data that show the flow is severely separated in the vicinity of the wing-pylon junction. Analysis shows that shock waves are induced by pylon swaybrace fairings, that the flow separation is much stronger at the outboard pylon and that the separation is caused mainly by the crossflow passing the geometry of wing-pylon junction.

  13. 1995 Bird survey Foothills parkway section 8B National Park Service, Tennessee

    SciTech Connect

    Wade, M.C.; Giffen, N.R.; Wade, B.A.

    1996-05-01

    The Foothills Parkway Section 8B right-of-way (ROW) is a stretch of land between Pittman Center and Cosby, Tennessee that is approximately 14.2 miles long and 1,000 ft wide, with a considerably wider section on Webb Mountain. A breeding bird survey was conducted at selected sample points along the ROW. The intent of the survey was to identify bird communities, area sensitive species (birds dependent on extensive forest systems for all their needs) and endangered, threatened, federal candidate, and state `in need of management` species now using the ROW. The survey also provides baseline data to assess future habitat impacts as well as cumulative impacts of the project.

  14. Searches for high frequency variations in the 8-B neutrino flux at the Sudbury neutrino observatory

    SciTech Connect

    Rielage, Keith; Seibert, Stanley R; Hime, Andrew; Elliott, Steven R; Stonehill, L C; Wouters, J M; Aharmim, B; Ahmed, S N; Anthony, A E; Barros, N; Beier, E W; Bellerive, A; Belttran, B; Bergevin, M; Biller, S D; Boudjemline, K; Burritt, T H; Cai, B; Chan, Y D; Chauhan, D; Chen, M; Cleveland, B T; Cox - Mobrand, G A; Dai, X; Deng, H; Detwiler, J; Dimarco, M; Doe, P J; Drouin, P - L; Duba, C A; Duncan, F A; Dunford, M; Earle, E D; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Ford, R J; Formaggilo, J A; Gagnon, N; Goon, J Tm; Guillian, E; Habib, S; Hahn, R L; Hallin, A L; Hallman, E D; Harvey, P J; Hazama, R; Heintzelman, W J; Heise, J; Helmer, R L; Howard, C; Howe, M A; Huang, M; Jamieson, B; Jelley, N A; Keeter, K J; Klein, J R; Kos, M; Kraus, C; Krauss, C B; Kutter, T; Kyba, C C M; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Loach, J C; Maclellan, R; Majerus, S; Mak, H B; Maneira, J; Martin, R; Mccauley, N; Mc Donald, A B; Mcgee, S; Miffin, C; Miller, M L; Monreal, B; Monroe, J; Morissette, B; Nickel, B G; Noble, A J; O' Keeffe, H M; Oblath, N S; Orebi Gann, G D; Oser, S M; Ott, R A; Peeters, S J M; Poon, A W P; Prior, G; Reitzner, S D; Robertson, B C; Robertson, R G H; Rollin, E; Schwendener, M H; Secrest, J A; Seibert, S R; Simard, O; Sinclair, D; Sinclair, L; Skensved, P; Sonley, T J; Tesic, G; Tolich, N; Tsui, T; Tunnell, C D; Van Berg, R; Van Devender, B A; Virtue, C J; Wall, B L; Waller, D; Wan Chan Tseung, H; West, N; Wilkerson, J F; Wilson, J R; Wright, A; Yeh, M; Zhang, F; Zuber, K

    2009-01-01

    We have peformed three searches for high-frequency signals in the solar neutrino flux measured by the Sudbury Neutrino Observatory (SNO), motivated by the possibility that solar g-mode oscillations could affect the production or propagation of solar {sup 8}B neutrinos. The first search looked for any significant peak in the frequency range l/day to 144/day, with a sensitivity to sinusoidal signals with amplitudes of 12% or greater. The second search focused on regions in which g-mode signals have been claimed by experiments aboard the SoHO satellite, and was sensitive to signals with amplitudes of 10% or greater. The third search looked for extra power across the entire frequency band. No statistically significant signal was detected in any of the three searches.

  15. The Coulomb Dissociation of {sup 8}B: A New Tool in Nuclear Astrophysics

    SciTech Connect

    Gai, Moshe

    2008-01-24

    The GSI1, GSI2 (as well as the RIKEN2 and the corrected GSI2) measurements of the Coulomb Dissociation (CD) of {sup 8}B are in good agreement with the most recent Direct Capture (DC) {sup 7}Be(p,{gamma}){sup 8}B reaction measurement performed at Weizmann and in agreement with the Seattle result. Yet it was claimed that the CD and DC results are sufficiently different and need to be reconciled. We show that these statements arise from a misunderstanding (as well as misrepresentation) of CD experiments. We recall a similar strong statement questioning the validity of the CD method due to an invoked large E2 component that was also shown to arise from a misunderstanding of the CD method. In spite of the good agreement between DC and CD data the slope of the astrophysical cross section factor (S{sub 17}) can not be extracted with high accuracy due to discrepancies among the most recent DC data as well as a discrepancies among the three reports of the GSI CD data. The slope is directly related to the d-wave component that dominates at higher energies. This d-wave component must be subtracted from measured data to extrapolate to zero energy. Hence the uncertainty of the measured slope leads to an additional downward uncertainty ({sub -3.0}{sup +0.0} eV-b) of the extrapolated zero energy cross section factor, S{sub 17}(0). Such an uncertainty is also consistent with the smaller value of S{sub 17}(0) extracted using the ANC method. This uncertainty must be alleviated by future experiments to allow a precise determination of S{sub 17}(0), a goal that so far has not be achieved in spite of strong statement(s) that appeared in the literature.

  16. Binding of the auxiliary subunit TRIP8b to HCN channels shifts the mode of action of cAMP.

    PubMed

    Hu, Lei; Santoro, Bina; Saponaro, Andrea; Liu, Haiying; Moroni, Anna; Siegelbaum, Steven

    2013-12-01

    Hyperpolarization-activated cyclic nucleotide-regulated cation (HCN) channels generate the hyperpolarization-activated cation current Ih present in many neurons. These channels are directly regulated by the binding of cAMP, which both shifts the voltage dependence of HCN channel opening to more positive potentials and increases maximal Ih at extreme negative voltages where voltage gating is complete. Here we report that the HCN channel brain-specific auxiliary subunit TRIP8b produces opposing actions on these two effects of cAMP. In the first action, TRIP8b inhibits the effect of cAMP to shift voltage gating, decreasing both the sensitivity of the channel to cAMP (K1/2) and the efficacy of cAMP (maximal voltage shift); conversely, cAMP binding inhibits these actions of TRIP8b. These mutually antagonistic actions are well described by a cyclic allosteric mechanism in which TRIP8b binding reduces the affinity of the channel for cAMP, with the affinity of the open state for cAMP being reduced to a greater extent than the cAMP affinity of the closed state. In a second apparently independent action, TRIP8b enhances the action of cAMP to increase maximal Ih. This latter effect cannot be explained by the cyclic allosteric model but results from a previously uncharacterized action of TRIP8b to reduce maximal current through the channel in the absence of cAMP. Because the binding of cAMP also antagonizes this second effect of TRIP8b, application of cAMP produces a larger increase in maximal Ih in the presence of TRIP8b than in its absence. These findings may provide a mechanistic explanation for the wide variability in the effects of modulatory transmitters on the voltage gating and maximal amplitude of Ih reported for different neurons in the brain.

  17. Probing the exotic structure of 8B by its elastic scattering and breakup reaction on nuclear targets

    NASA Astrophysics Data System (ADS)

    Lukyanov, V. K.; Kadrev, D. N.; Zemlyanaya, E. V.; Lukyanov, K. V.; Antonov, A. N.; Gaidarov, M. K.; Spasova, K.

    2017-02-01

    The structure of the exotic 8B nucleus is studied by means of elastic scattering, as well as its breakup on nuclear targets. We present microscopic calculations of the optical potentials (OPs) and cross sections of elastic scattering of 8B on 12C, 58Ni, and 208Pb targets at energies 20 < E < 170 MeV. The density distributions of 8B obtained within the variational Monte Carlo (VMC) model and the three-cluster model (3CM) are used to construct the potentials. The real part of the hybrid OP is calculated using the folding model with the direct and exchange terms included, while the imaginary part is obtained on the base of the high-energy approximation (HEA) and also taken to be equal to the microscopic real part of the OP. In this model the only free parameters are the depths of the real and imaginary parts of OP obtained by fitting the elastic scattering experimental data. A dependence of their values on the model density of 8 B is found. In addition, the cluster model, in which 8B consists of a p-halo and the 7 Be core, is applied to calculate the breakup cross sections of the 8B nucleus on 9Be, 12C, and 197Au targets, as well as momentum distributions of 7Be fragments, and a comparison with the existing experimental data is made.

  18. JT8D-15/17 High Pressure Turbine Root Discharged Blade Performance Improvement. [engine design

    NASA Technical Reports Server (NTRS)

    Janus, A. S.

    1981-01-01

    The JT8D high pressure turbine blade and seal were modified, using a more efficient blade cooling system, improved airfoil aerodynamics, more effective control of secondary flows, and improved blade tip sealing. Engine testing was conducted to determine the effect of these improvements on performance. The modified turbine package demonstrated significant thrust specific fuel consumption and exhaust gas temperature improvements in sea level and altitude engine tests. Inspection of the improved blade and seal hardware after testing revealed no unusual wear or degradation.

  19. JT8D and JT9D jet engine performance improvement program. Task 1: Feasibility analysis

    NASA Technical Reports Server (NTRS)

    Gaffin, W. O.; Webb, D. E.

    1979-01-01

    JT8D and JT9D component performance improvement concepts which have a high probability of incorporation into production engines were identified and ranked. An evaluation method based on airline payback period was developed for the purpose of identifying the most promising concepts. The method used available test data and analytical models along with conceptual/preliminary designs to predict the performance improvements, weight, installation characteristics, cost for new production and retrofit, maintenance cost, and qualitative characteristics of candidate concepts. These results were used to arrive at the concept payback period, which is the time required for an airline to recover the investment cost of concept implementation.

  20. 75 FR 50945 - Airworthiness Directives; Pratt & Whitney JT8D-209, -217, -217A, -217C, and -219 Turbofan Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... JT8D-209, -217, -217A, -217C, and -219 Turbofan Engines AGENCY: Federal Aviation Administration (FAA... airworthiness directive (AD) for Pratt & Whitney (PW) JT8D-209, -217, -217A, -217C, and -219 turbofan engines. That AD currently requires revisions to the engine manufacturer's time limits section (TLS) to...

  1. 13 CFR 121.411 - What are the size procedures for SBA's Section 8(d) Subcontracting Program?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...'s Section 8(d) Subcontracting Program? (a) Prime contractors may rely on the information contained... still qualify and self-certify as a small business at the time it submits its offer as a section 8(d... prior to award, the prime contractor must inform each unsuccessful subcontract offeror in writing of...

  2. The two Dictyostelium autophagy eight proteins, ATG8a and ATG8b, associate with the autophagosome in succession.

    PubMed

    Matthias, Jan; Meßling, Susanne; Eichinger, Ludwig

    2016-01-01

    Autophagy is an ancient cellular pathway that is conserved from yeast to man. It contributes to many physiological and pathological processes and plays a major role in the degradation of proteins and/or organelles in response to starvation and stress. In the autophagic process cytosolic material is captured into double membrane-bound vesicles, the autophagosomes. After fusion with lysosomes, the cargo is degraded in the generated autolysosomes and then recycled for further use. Autophagy 8 (ATG8, in mammals LC3), a well-established marker of autophagy, is covalently linked to phosphatidylethanolamine on the autophagic membrane during autophagosome formation. Bioinformatic analysis of the Dictyostelium genome revealed two atg8 genes which encode the ATG8a and ATG8b paralogs. They are with around 14kDa similar in size, 54 % identical to one another and more closely related to the corresponding proteins in fungi and plants than in animals. For ATG8a we found a strong up-regulation throughout the 24h developmental time course while ATG8b expression was highest in vegetative cells followed by a moderate reduction during early development. Confocal microscopy of fluorescently tagged ATG8a and ATG8b in vegetative AX2 wild-type and in ATG9(-) cells showed that both proteins mainly co-localized on vesicular structures with a diameter above 500nm while those smaller than 500nm were predominantly positive for ATG8b. In ATG9(-) cells we found a strong increase in the relative abundance of ATG8a-positive large vesicular structures and of total ATG8b-positive structures per cell indicating autophagic flux problems in this mutant. We also found that vesicular structures positive for ATG8a and/or ATG8b were also positive for ubiquitin. Live cell imaging of AX2 and ATG9(-) cells co-expressing combinations of red and green tagged ATG8a, ATG8b or ATG9 revealed transient co localizations of these proteins. Our results suggest that ATG8b associates with nascent autophagosomes before

  3. Performance of aluminide coatings applied on alloy CF8C plus at 800 C

    SciTech Connect

    Kumar, Deepak; Dryepondt, Sebastien N; Shyam, Amit; Haynes, James A; Pint, Bruce A; Armstrong, Beth L; Lara-Curzio, Edgar

    2011-01-01

    The cost effective, austenitic stainless steel CF8C plus is an attractive alloy for massive cast structures such as steam turbine casings. The microstructure stability and creep strength of this alloy are better than commercial high-performance heat-resistant steels such as NF709 and Super 304H, and are comparable to the Ni-based superalloy Inconel 617. The oxidation resistance of the alloy in atmosphere rich in water vapor is however insufficient at T>800 C, and the use of diffusion aluminide coatings is considered for potential high temperature applications. The thermal stability and protectiveness of coatings applied on the CF8C plus substrate by pack cementation and slurry process were investigated in air + 10% H2O environment at 800 C. Further, the coating effect on the fatigue life of the alloy was assessed via low-cycle-fatigue experiments.

  4. Lateral aerodynamic parameters extracted from flight data for the F-8C airplane in maneuvering flight

    NASA Technical Reports Server (NTRS)

    Suit, W. T.

    1977-01-01

    Flight test data are used to extract the lateral aerodynamic parameters of the F-8C airplane at moderate to high angles of attack. The data were obtained during perturbations of the airplane from steady turns with trim normal accelerations from 1.5g to 3.0g. The angle-of-attack variation from trim was negligible. The aerodynamic coefficients extracted from flight data were compared with several other sets of coefficients, and the extracted coefficients resulted in characteristics for the Dutch roll mode (at the highest angles of attack) similar to those of a set of coefficients that have been the basis of several simulations of the F-8C.

  5. Pressure distribution for the wing of the YAV-8B airplane; with and without pylons

    NASA Technical Reports Server (NTRS)

    Saltzman, Edwin J.; Delfrate, John H.; Sabsay, Catherine M.; Yarger, Jill M.

    1992-01-01

    Pressure distribution data have been obtained in flight at four span stations on the wing panel of the YAV-8B airplane. Data obtained for the supercritical profiled wing, with and without pylons installed, ranged from Mach 0.46 to 0.88. The altitude ranged from approximately 20,000 to 40,000 ft and the resultant Reynolds numbers varied from approximately 7.2 million to 28.7 million based on the mean aerodynamic chord. Pressure distribution data and flow visualization results show that the full-scale flight wing performance is compromised because the lower surface cusp region experiences flow separation for some important transonic flight conditions. This condition is aggravated when local shocks occur on the lower surface of the wing (mostly between 20 and 35 percent chord) when the pylons are installed for Mach 0.8 and above. There is evidence that convex fairings, which cover the pylon attachment flanges, cause these local shocks. Pressure coefficients significantly more negative than those for sonic flow also occur farther aft on the lower surface (near 60 percent chord) whether or not the pylons are installed for Mach numbers greater than or equal to 0.8. These negative pressure coefficient peaks and associated local shocks would be expected to cause increasing wave and separation drag at transonic Mach number increases.

  6. DC-9 flight demonstration program with refanned JT8D engines. Volume 4: Flyover noise

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Flyover noise tests were conducted to determine the noise reductions achievable by modifying the engines and nacelles of DC-9-30 airplanes. The two stage fan of the JT8D-9 engine was replaced with a larger diameter, single stage fan and sound absorbing materials were incorporated in the engines and nacelles. The noise levels were determined to be 95.3 EPNdB at the sideline, 96.2 EPNdB for a full thrust takeoff, 87.5 EPNdB for takeoff with thrust cutback, and 97.4 EPNdB for landing approach. The noise reductions relative to the hardwall JT8D-9 were 8.2 EPNdB for takeoff with cutback and 8.7 EPNdB for landing. The 90 EPNdB noise contour areas were reduced by 40% for missions requiring maximum design takeoff and landing weights. For typical mission weights, the reductions were 19% for full thrust takeoff and 34% for takeoff with cutback. The 95 EPNdB contour areas were reduced by 50% for takeoff and 30% for takeoff with cutback for both missions.

  7. DC-9 Flight Demonstration Program with Refanned JT8D Engines. Volume 3; Performance and Analysis

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The JT8D-109 engine has a sea level static, standard day bare engine takeoff thrust of 73,840 N. At sea level standard day conditions the additional thrust of the JT8D-109 results in 2,040 kg additional takeoff gross weight capability for a given field length. Range loss of the DC-9 Refan airplane for long range cruise was determined. The Refan airplane demonstrated stall, static longitudinal stability, longitudinal control, longitudinal trim, minimum control speeds, and directional control characteristics similar to the DC-9-30 production airplane and complied with airworthiness requirements. Cruise, climb, and thrust reverser performance were evaluated. Structural and dynamic ground test, flight test and analytical results substantiate Refan Program requirements that the nacelle, thrust reverser hardware, and the airplane structural modifications are flightworthy and certifiable and that the airplane meets flutter speed margins. Estimated unit cost of a DC-9 Refan retrofit program is 1.338 million in mid-1975 dollars with about an equal split in cost between airframe and engine.

  8. Wibault two-seat monoplane 8C2 : an all-metal pursuit and observation airplane

    NASA Technical Reports Server (NTRS)

    Serryer, J

    1926-01-01

    Michel Wibault's two seat monoplane 8C2, is similar to the Parasol pursuit monoplane which preceded it. It has no perishable parts in its structure and needs no special storage or coverings. The sample aeroplane uses a 500 HP Hispano-Suiza engine but can accept a 400-600HP engine from a variety of manufacturers with little difficulty. It uses a two blade tractor propeller.

  9. Structural basis for the mutual antagonism of cAMP and TRIP8b in regulating HCN channel function.

    PubMed

    Saponaro, Andrea; Pauleta, Sofia R; Cantini, Francesca; Matzapetakis, Manolis; Hammann, Christian; Donadoni, Chiara; Hu, Lei; Thiel, Gerhard; Banci, Lucia; Santoro, Bina; Moroni, Anna

    2014-10-07

    cAMP signaling in the brain mediates several higher order neural processes. Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels directly bind cAMP through their cytoplasmic cyclic nucleotide binding domain (CNBD), thus playing a unique role in brain function. Neuronal HCN channels are also regulated by tetratricopeptide repeat-containing Rab8b interacting protein (TRIP8b), an auxiliary subunit that antagonizes the effects of cAMP by interacting with the channel CNBD. To unravel the molecular mechanisms underlying the dual regulation of HCN channel activity by cAMP/TRIP8b, we determined the NMR solution structure of the HCN2 channel CNBD in the cAMP-free form and mapped on it the TRIP8b interaction site. We reconstruct here the full conformational changes induced by cAMP binding to the HCN channel CNBD. Our results show that TRIP8b does not compete with cAMP for the same binding region; rather, it exerts its inhibitory action through an allosteric mechanism, preventing the cAMP-induced conformational changes in the HCN channel CNBD.

  10. DISCOVERY AND ROSSITER-McLAUGHLIN EFFECT OF EXOPLANET KEPLER-8b

    SciTech Connect

    Jenkins, Jon M.; Caldwell, Douglas A.; Marcy, Geoffrey W.; Basri, Gibor; Isaacson, Howard; Cochran, William D.; Endl, Michael; Welsh, William F.; Batalha, Natalie M.; Buchhave, Lars A.; Geary, John C.; Brown, Timothy M.; Dunham, Edward W.; Fischer, Debra A.; Gautier, Thomas N.; Howell, Steve B.; Johnson, John Asher

    2010-12-01

    We report on the discovery and the Rossiter-McLaughlin (R-M) effect of Kepler-8b, a transiting planet identified by the NASA Kepler Mission. Kepler photometry and Keck-HIRES radial velocities yield the radius and mass of the planet around this F8IV subgiant host star. The planet has a radius R{sub P} = 1.419 R{sub J} and a mass M{sub P} = 0.60 M{sub J}, yielding a density of 0.26 g cm{sup -3}, one of the lowest planetary densities known. The orbital period is P = 3.523 days and the orbital semimajor axis is 0.0483{sup +0.0006}{sub -0.0012} AU. The star has a large rotational vsin i of 10.5 {+-} 0.7 km s{sup -1} and is relatively faint (V {approx} 13.89 mag); both properties are deleterious to precise Doppler measurements. The velocities are indeed noisy, with scatter of 30 m s{sup -1}, but exhibit a period and phase that are consistent with those implied by transit photometry. We securely detect the R-M effect, confirming the planet's existence and establishing its orbit as prograde. We measure an inclination between the projected planetary orbital axis and the projected stellar rotation axis of {lambda} = -26.{sup 0}4 {+-} 10.{sup 0}1, indicating a significant inclination of the planetary orbit. R-M measurements of a large sample of transiting planets from Kepler will provide a statistically robust measure of the true distribution of spin-orbit orientations for hot Jupiters around F and early G stars.

  11. Evidence for a functional subdivision of Premotor Ear-Eye Field (Area 8B)

    PubMed Central

    Lanzilotto, Marco; Perciavalle, Vincenzo; Lucchetti, Cristina

    2015-01-01

    The Supplementary Eye Field (SEF) and the Frontal Eye Field (FEF) have been described as participating in gaze shift control. Recent evidence suggests, however, that other areas of the dorsomedial prefrontal cortex also influence gaze shift. Herein, we have investigated electrically evoked ear- and eye movements from the Premotor Ear-Eye Field, or PEEF (area 8B) of macaque monkeys. We stimulated PEEF during spontaneous condition (outside the task performance) and during the execution of a visual fixation task (VFT). In the first case, we functionally identified two regions within the PEEF: a core and a belt. In the core region, stimulation elicited forward ear movements; regarding the evoked eye movements, in some penetrations, stimulation elicited contraversive fixed-vectors with a mean amplitude of 5.14°; while in other penetrations, we observed prevalently contralateral goal-directed eye movements having end-points that fell within 15° in respect to the primary eye position. On the contrary, in the belt region, stimulation elicited backward ear movements; regarding the eye movements, in some penetrations stimulation elicited prevalently contralateral goal-directed eye movements having end-points that fell within 15° in respect to the primary eye position, while in the lateral edge of the investigated region, stimulation elicited contralateral goal-directed eye movements having end-points that fell beyond 15° in respect to the primary eye position. Stimulation during VFT either did not elicit eye movements or evoked saccades of only a few degrees. Finally, even though no head rotation movements were observed during the stimulation period, we viewed a relationship between the duration of stimulation and the neck forces exerted by the monkey's head. We propose an updated vision of the PEEF composed of two functional regions, core and belt, which may be involved in integrating auditory and visual information important to the programming of gaze orienting

  12. Discovery and Rossiter-Mclaughlin Effect of Exoplanet Kepler-8b

    NASA Astrophysics Data System (ADS)

    Jenkins, Jon M.; Borucki, William J.; Koch, David G.; Marcy, Geoffrey W.; Cochran, William D.; Welsh, William F.; Basri, Gibor; Batalha, Natalie M.; Buchhave, Lars A.; Brown, Timothy M.; Caldwell, Douglas A.; Dunham, Edward W.; Endl, Michael; Fischer, Debra A.; Gautier, Thomas N., III; Geary, John C.; Gilliland, Ronald L.; Howell, Steve B.; Isaacson, Howard; Johnson, John Asher; Latham, David W.; Lissauer, Jack J.; Monet, David G.; Rowe, Jason F.; Sasselov, Dimitar D.; Howard, Andrew W.; MacQueen, Phillip; Orosz, Jerome A.; Chandrasekaran, Hema; Twicken, Joseph D.; Bryson, Stephen T.; Quintana, Elisa V.; Clarke, Bruce D.; Li, Jie; Allen, Christopher; Tenenbaum, Peter; Wu, Hayley; Meibom, Søren; Klaus, Todd C.; Middour, Christopher K.; Cote, Miles T.; McCauliff, Sean; Girouard, Forrest R.; Gunter, Jay P.; Wohler, Bill; Hall, Jennifer R.; Ibrahim, Khadeejah; Kamal Uddin, AKM; Wu, Michael S.; Bhavsar, Paresh A.; Van Cleve, Jeffrey; Pletcher, David L.; Dotson, Jessie L.; Haas, Michael R.

    2010-12-01

    We report on the discovery and the Rossiter-McLaughlin (R-M) effect of Kepler-8b, a transiting planet identified by the NASA Kepler Mission. Kepler photometry and Keck-HIRES radial velocities yield the radius and mass of the planet around this F8IV subgiant host star. The planet has a radius R P = 1.419 R J and a mass M P = 0.60 M J, yielding a density of 0.26 g cm-3, one of the lowest planetary densities known. The orbital period is P = 3.523 days and the orbital semimajor axis is 0.0483+0.0006 -0.0012 AU. The star has a large rotational vsin i of 10.5 ± 0.7 km s-1 and is relatively faint (V ≈ 13.89 mag); both properties are deleterious to precise Doppler measurements. The velocities are indeed noisy, with scatter of 30 m s-1, but exhibit a period and phase that are consistent with those implied by transit photometry. We securely detect the R-M effect, confirming the planet's existence and establishing its orbit as prograde. We measure an inclination between the projected planetary orbital axis and the projected stellar rotation axis of λ = -26fdg4 ± 10fdg1, indicating a significant inclination of the planetary orbit. R-M measurements of a large sample of transiting planets from Kepler will provide a statistically robust measure of the true distribution of spin-orbit orientations for hot Jupiters around F and early G stars. Based in part on observations obtained at the W. M. Keck Observatory, which is operated as a scientific partnership between the California Institute of Technology, the University of California, and the National Aeronautics and Space Administration. The Observatory was made possible by the generous financial support of the W. M. Keck Foundation.

  13. Chamigrane Sesquiterpenes from a Basidiomycetous Endophytic Fungus XG8D Associated with Thai Mangrove Xylocarpus granatum

    PubMed Central

    Choodej, Siwattra; Teerawatananond, Thapong; Mitsunaga, Tohru; Pudhom, Khanitha

    2016-01-01

    Six new chamigrane sesquiterpenes, merulinols A‒F (1‒6), and four known metabolites (7‒10) were isolated from the culture of the basidiomycetous fungus XG8D, a mangrove-derived endophyte. Their structures were elucidated mainly by 1D and 2D NMR, while the structures of 1 and 2 were further confirmed by single-crystal X-ray diffraction analysis. The in vitro cytotoxicity of all compounds was evaluated against three human cancer cell lines, MCF-7, Hep-G2, and KATO-3. Compounds 3 and 4 selectively displayed cytotoxicity against KATO-3 cells with IC50 values of 35.0 and 25.3 μM, respectively. PMID:27428984

  14. JT8D revised high-pressure turbine cooling and other outer air seal program

    NASA Technical Reports Server (NTRS)

    Gaffin, W. O.

    1979-01-01

    The JT8D high pressure turbine was revised to reduce leakage between the blade tip shrouds and the outer air seal, and engine testing was performed to determine the effect on performance. The addition of a second knife-edge on the blade tip shroud, the extension of the honeycomb seal land to cover the added knife-edge and an existing spoiler on the shroud, and a material substitution in the seal support ring to improve thermal growth characteristics are included. A relocation of the blade cooling air discharge to insure adequate cooling flow is required. Significant specific fuel consumption and exhaust gas temperature improvements were demonstrated with the revised turbine in sea level and simulated altitude engine tests. Inspection of the revised seal hardware after these tests showed no unusual wear or degradation.

  15. The 727 airplane target thrust reverser static performance model test for refanned JT8D engines

    NASA Technical Reports Server (NTRS)

    Chow, C. T. P.; Atkey, E. N.

    1974-01-01

    The results of a scale model static performance test of target thrust reverser configurations for the Pratt and Whitney Aircraft JT8D-100 series engine are presented. The objective of the test was to select a series of suitable candidate reverser configurations for the subsequent airplane model wind tunnel ingestion and flight controls tests. Test results indicate that adequate reverse thrust performance with compatible engine airflow match is achievable for the selected configurations. Tapering of the lips results in loss of performance and only minimal flow directivity. Door pressure surveys were conducted on a selected number of lip and fence configurations to obtain data to support the design of the thrust reverser system.

  16. Search for periodicities in the {sup 8}B solar neutrino flux measured by the Sudbury Neutrino Observatory

    SciTech Connect

    Aharmim, B.; Farine, J.; Fleurot, F.; Hallman, E.D.; Krueger, A.; Luoma, S.; Schwendener, M.H.; Tafirout, R.; Virtue, C.J.; Ahmed, S.N.; Chen, M.; Duncan, F.A.; Earle, E.D.; Evans, H.C.; Ewan, G.T.; Fulsom, B.G.; Graham, K.; Hallin, A.L.; Handler, W.B.; Harvey, P.J.

    2005-09-01

    A search has been made for sinusoidal periodic variations in the {sup 8}B solar neutrino flux using data collected by the Sudbury Neutrino Observatory over a 4-year time interval. The variation at a period of 1 yr is consistent with modulation of the {sup 8}B neutrino flux by the Earth's orbital eccentricity. No significant sinusoidal periodicities are found with periods between 1 d and 10 years with either an unbinned maximum likelihood analysis or a Lomb-Scargle periodogram analysis. The data are inconsistent with the hypothesis that the results of the recent analysis by Sturrock et al., based on elastic scattering events in Super-Kamiokande, can be attributed to a 7% sinusoidal modulation of the total {sup 8}B neutrino flux.

  17. Quasielastic scattering of 8B and 7Be on 12C at 40 MeV/nucleon

    NASA Astrophysics Data System (ADS)

    Pecina, I.; Anne, R.; Bazin, D.; Borcea, C.; Borrel, V.; Carstoiu, F.; Corre, J. M.; Dlouhy, Z.; Fomitchev, A.; Guillemaud-Mueller, D.; Keller, H.; Kordyasz, A.; Lewitowicz, M.; Lukyanov, S.; Mueller, A. C.; Penionzhkevich, Yu.; Roussel-Chomaz, P.; Saint-Laurent, M. G.; Skobelev, N.; Sorlin, O.; Tarasov, O.

    1995-07-01

    The quasielastic scattering of the exotic nucleus 8B on a 12C target has been studied at an energy of 320 MeV and compared with that of 7Be at the same velocity. The quasielastic scattering of 12C + 12C at 20 MeV/nucleon, also performed as a secondary beam experiment, was used to check the data reduction method. The results are interpreted in terms of a semimicroscopic double folding model and coupled-channels calculation. The difference in the total reaction cross section (8%) between 8B and 7Be is consistent with the measured one proton removal cross section and corresponds to an increase in the interaction radius of 4%. The existence of a substantial proton halo in 8B is not supported by the present data.

  18. Cyp8b1 ablation prevents western diet-induced weight gain and hepatic steatosis due to impaired fat absorption.

    PubMed

    Bertaggia, Enrico; Jensen, Kristian K; Castro-Perez, Jose; Xu, Yimeng; Di Paolo, Gilbert; Chan, Robin B; Wang, Liangsu; Haeusler, Rebecca A

    2017-04-04

    Bile acids (BAs) are cholesterol derivatives that regulate lipid metabolism, through their dual abilities to promote lipid absorption and activate BA receptors. However, different BA species have varying abilities to perform these functions. Eliminating 12α-hydroxy BAs in mice via Cyp8b1 knockout causes low body weight and improved glucose tolerance. The goal of this study was to determine mechanisms of low body weight in Cyp8b1(-/-) mice. We challenged Cyp8b1(-/-) mice with western type diet and assessed body weight and composition. We measured energy expenditure, fecal calories, lipid absorption and performed lipidomic studies on feces and intestine. We investigated the requirement for dietary fat in the phenotype using a fat-free diet. Cyp8b1(-/-) mice were resistant to western diet-induced body weight gain, hepatic steatosis, and insulin resistance. These changes were associated with increased fecal calories, due to malabsorption of hydrolyzed dietary triglycerides. This was reversed by treating the mice with taurocholic acid, the major 12α-hydroxylated BA species. The improvements in body weight and steatosis were normalized by feeding mice a fat-free diet. The effects of BA composition on intestinal lipid handling are important for whole-body energy homeostasis. Thus, modulating BA composition is a potential tool for obesity or diabetes therapy.

  19. SEARCH FOR GLOBAL f-MODES AND p-MODES IN THE {sup 8}B NEUTRINO FLUX

    SciTech Connect

    Lopes, Ilídio E-mail: ilopes@uevora.pt

    2013-11-01

    The impact of global acoustic modes on the {sup 8}B neutrino flux time series is computed for the first time. It is shown that the time fluctuations of the {sup 8}B neutrino flux depend on the amplitude of acoustic eigenfunctions in the region where the {sup 8}B neutrino flux is produced: modes with low n (or order) that have eigenfunctions with a relatively large amplitude in the Sun's core strongly affect the neutrino flux; conversely, modes with high n that have eigenfunctions with a minimal amplitude in the Sun's core have a very small impact on the neutrino flux. It was found that the global modes with a larger impact on the {sup 8}B neutrino flux have a frequency of oscillation in the interval 250 μHz to 500 μHz (or a period in the interval 30 minutes to 70 minutes), such as the f-modes (n = 0) for the low degrees, radial modes of order n ≤ 3, and the dipole mode of order n = 1. Their corresponding neutrino eigenfunctions are very sensitive to the solar inner core and are unaffected by the variability of the external layers of the solar surface. If time variability of neutrinos is observed for these modes, it will lead to new ways of improving the sound speed profile inversion in the central region of the Sun.

  20. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... statements of face-amount certificate companies. 274.14 Section 274.14 Commodity and Securities Exchanges... Registration Statements § 274.14 Form N-8B-4, registration statements of face-amount certificate companies. This form shall be used for registration statements of face-amount certificate companies...

  1. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... statements of face-amount certificate companies. 274.14 Section 274.14 Commodity and Securities Exchanges... Registration Statements § 274.14 Form N-8B-4, registration statements of face-amount certificate companies. This form shall be used for registration statements of face-amount certificate companies...

  2. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... statements of face-amount certificate companies. 274.14 Section 274.14 Commodity and Securities Exchanges... Registration Statements § 274.14 Form N-8B-4, registration statements of face-amount certificate companies. This form shall be used for registration statements of face-amount certificate companies...

  3. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... statements of face-amount certificate companies. 274.14 Section 274.14 Commodity and Securities Exchanges... Registration Statements § 274.14 Form N-8B-4, registration statements of face-amount certificate companies. This form shall be used for registration statements of face-amount certificate companies...

  4. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... statements of face-amount certificate companies. 274.14 Section 274.14 Commodity and Securities Exchanges... Registration Statements § 274.14 Form N-8B-4, registration statements of face-amount certificate companies. This form shall be used for registration statements of face-amount certificate companies...

  5. Gaseous exhaust emissions from a JT8D-109 turbofan engine at simulated cruise flight conditions

    NASA Technical Reports Server (NTRS)

    Diehl, L. A.; Holdeman, J. D.

    1975-01-01

    Gaseous emissions from a JT8D-109 turbofan engine were measured in an altitude facility at four simulated cruise flight conditions: Mach 0.8 at altitudes of 9.1, 10, 7, and 12.2 km and Mach 0.9 at 10.7 km. Engine inlet air temperature was held constant at 283 K for all tests. Emissions measurements were made at nominally 6 cm intervals across the horizontal diameter of the engine exhaust nozzle with a single-point traversing gas sample probe. Measured emissions of decreased with increasing altitude from an emission index of 10.4 to one of 8.3, while carbon monoxide increased with increasing altitude from an emission index of 1.6 to one of 4.4. Unburned hydrocarbon emissions were essentially negligible for all flight conditions. Since the engine inlet air temperatures were not correctly simulated, the NOx emission indices were corrected to true altitude conditions by using correlating parameters for changes in combustor inlet temperature, pressure, and temperature rise. The correction was small at the lowest altitude. At the 10.7 and 12.2 km, Mach 0.8 test conditions the correction decreased the measured values by 1 emission index.

  6. Predicted infrared and Raman spectra for neutral Ti{sub 8}C{sub 12} isomers

    SciTech Connect

    Baruah, Tunna; Pederson, Mark R.; Lyn, M.L.; Castleman, A.W. Jr.

    2002-11-01

    Using a density-functional based algorithm, the full infrared and Raman spectra are calculated for the neutral Ti{sub 8}C{sub 12} cluster assuming geometries of T{sub h}, T{sub d}, D{sub 2d}, and C{sub 3v} symmetry. The T{sub h} pentagonal dodecahedron is found to be dynamically unstable. The calculated properties of the relaxed structure having C{sub 3v} symmetry are found to be in excellent agreement with experimental gas-phase infrared results, ionization potential and electron affinity measurements. Consequently, the results presented may be used as a reference for further experimental characterization using vibrational spectroscopy.

  7. The Features of GGT in Patients with ATP8B1 or ABCB11 Deficiency Improve the Diagnostic Efficiency

    PubMed Central

    Wang, Neng-Li; Li, Li-Ting; Wu, Bing-Bing; Gong, Jing-Yu; Abuduxikuer, Kuerbanjiang; Li, Gang; Wang, Jian-She

    2016-01-01

    Background and Aims Genetic defects in ATP8B1 or ABCB11 account for the majority of cholestasis with low GGT. But the ranges for GGT in patients with ATP8B1 or ABCB11 deficiency are unclear. This study tried to unravel the features of GGT in these patients that improve diagnostic efficiency. Methods This study enrolled 207 patients with chronic cholestasis who were ordered to test for ATP8B1 and/or ABCB11 from January 2012 to December 2015. Additional 17 patients with ATPB81 or ABCB11 deficiency diagnosed between January 2004 and December 2011 were also enrolled in this study. 600 population-matched children served as controls. Clinical data were obtained by retrospectively reviewing medical records. Results A total of 26 patients were diagnosed with ATP8B1 deficiency and 30 patients were diagnosed with ABCB11 deficiency. GGT levels were similar between the two disorders at any observed month of age, but varied with age. The peak GGT value was <70U/L in the 2nd~6th month of life, <60U/L in the 7th~12th month and <50U/L beyond one year. GGT levels in patients with a genetic diagnosis were different from that in patients without a genetic diagnosis and controls. Larger ranges for GGT were found in patients without a genetic diagnosis. Some controls had GGT≥70U/L in the 2nd~6th month. Of the 207 patients, 39 (18.8%) obtained a genetic diagnosis. 111 patients met the ranges described above, including all the 39 patients with ATP8B1 or ABCB11 deficiency. The sensitivity was 100.0%. The rate of a positive molecular diagnosis increased to 35.1% (39/111 vs. 39/207, X2 = 10.363, P = 0.001). The remaining 96 patients exceeded the ranges described above and failed to receive a genetic diagnosis. These patients accounted for 43.8% of sequencing cost. Conclusions GGT levels in patients with ATP8B1 or ABCB11 deficiency varied with age. The peak GGT value was <70U/L in the 2nd~6th month of life, <60U/L in the 7th~12th month and <50U/L beyond one year. PMID:27050426

  8. [ital E]2 properties of nuclei far from stability and the proton-halo problem of [sup 8]B

    SciTech Connect

    Nakada, H.; Otsuka, T. Department of Physics, Faculty of Science, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113 Institute for Nuclear Theory, University of Washington, NH-12, Seattle, Washington 98195 )

    1994-02-01

    [ital E]2 properties of [ital A]=6--10 nuclei, including those of nuclei far from stability, are studied by a (0+2)[h bar][omega] shell-model calculation which includes [ital E]2 core-polarization effects explicitly. The quadrupole moments and the [ital E]2 transition strengths in [ital A]=6--10 nuclei are described quite well by the present cal- culation. This result indicates that the relatively large value of the quadrupole moment of [sup 8]B can be understood without introducing the proton halo in [sup 8]B. An interesting effect of the 2[h bar][omega] core-polarization is found for effective charges used in the 0[h bar][omega] shell model; although isoscalar effective charges are almost constant as a function of nucelus, appreciable variations are needed for isovector effective charges which play important roles in nuclei with high isospin values.

  9. Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea

    PubMed Central

    Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; Sà, Elisabet Laia; Ignacio-Espinoza, J. Cesar; Cornejo-Castillo, Francisco M.; Verberkmoes, Nathan C.; Vaqué, Dolors; Sullivan, Matthew B.; Acinas, Silvia G.

    2015-01-01

    Marine viruses (phages) alter bacterial diversity and evolution with impacts on marine biogeochemical cycles, and yet few well-developed model systems limit opportunities for hypothesis testing. Here we isolate phage B8b from the Mediterranean Sea using Pseudoalteromonas sp. QC-44 as a host and characterize it using myriad techniques. Morphologically, phage B8b was classified as a member of the Siphoviridae family. One-step growth analyses showed that this siphovirus had a latent period of 70 min and released 172 new viral particles per cell. Host range analysis against 89 bacterial host strains revealed that phage B8b infected 3 Pseudoalteromonas strains (52 tested, >99.9% 16S rRNA gene nucleotide identity) and 1 non-Pseudoaltermonas strain belonging to Alteromonas sp. (37 strains from 6 genera tested), which helps bound the phylogenetic distance possible in a phage-mediated horizontal gene transfer event. The Pseudoalteromonas phage B8b genome size was 42.7 kb, with clear structural and replication modules where the former were delineated leveraging identification of 16 structural genes by virion structural proteomics, only 4 of which had any similarity to known structural proteins. In nature, this phage was common in coastal marine environments in both photic and aphotic layers (found in 26.5% of available viral metagenomes), but not abundant in any sample (average per sample abundance was 0.65% of the reads). Together these data improve our understanding of siphoviruses in nature, and provide foundational information for a new ‘rare virosphere’ phage–host model system. PMID:25587991

  10. Whole-exome Sequencing Identifies Rare Variants in ATP8B4 as a Risk Factor for Systemic Sclerosis

    PubMed Central

    Gao, Li; Emond, Mary J; Louie, Tin; Cheadle, Chris; Berger, Alan E.; Rafaels, Nicholas; Vergara, Candelaria; Kim, Yoonhee; Taub, Margaret A.; Ruczinski, Ingo; Mathai, Stephen C.; Rich, Stephen S; Nickerson, Deborah A; Hummers, Laura K.; Bamshad, Michael J; Hassoun, Paul M.; Mathias, Rasika A; Barnes, Kathleen C.

    2015-01-01

    Objective To determine the contribution of rare variants as genetic modifiers of the expressivity, penetrance, and severity of systemic sclerosis (SSc). Methods We performed whole-exome sequencing of 78 European American systemic sclerosis patients, including 35 patients without pulmonary arterial hypertension (SSc-PAH−) and 43 patients with PAH (SSc-PAH+). Association testing of case-control probability for rare variants was performed using the aSKAT-O method with small sample adjustment by comparing all SSc patients with a reference population of 3,179 controls from the ESP 5,500 exome dataset. Replication genotyping was performed in an independent sample of 3,263 patients (415 SSc and 2,848 controls). We conducted expression profiling of mRNA from 61 SSc patients (19 SSc-PAH− and 42 SSc-PAH+) and 41 corresponding controls. Results The ATP8B4 gene was associated with a significant increase in the risk of SSc (P = 3.18 × 10−7). Among the 64 ATP8B4 variants tested, a single missense variant, c.1308C>G (F436L, rs55687265), provided the most compelling evidence for association (P = 9.35 × 10−10; OR = 6.11), which was confirmed in the replication cohort (P = 0.012; OR = 1.86) and meta-analysis (P = 1.92 x 10−7; OR = 2.5). Genes involved in E3 ubiquitin-protein ligase complex (ASB10) and cyclic nucleotide gated channelopathies (CNGB3) as well as HLA-DRB5 and HSPB2 (aka heat shock protein 27) provided additional evidence for association (P < 10−5). Differential ATP8B4 expression was observed among the SSc patients compared to the controls (P = 0.0005). Conclusion ATP8B4 may represent a putative genetic risk factor for SSc and pulmonary vascular complications. PMID:26473621

  11. Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea

    DOE PAGES

    Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; ...

    2015-01-14

    Marine viruses (phages) alter bacterial diversity and evolution with impacts on marine biogeochemical cycles, and yet few well-developed model systems limit opportunities for hypothesis testing. We isolate phage B8b from the Mediterranean Sea using Pseudoalteromonas sp. QC-44 as a host and characterize it using myriad techniques. Morphologically, phage B8b was classified as a member of the Siphoviridae family. One-step growth analyses showed that this siphovirus had a latent period of 70 min and released 172 new viral particles per cell. In the host range analysis against 89 bacterial host strains revealed that phage B8b infected 3 Pseudoalteromonas strains (52 tested,more » >99.9% 16S rRNA gene nucleotide identity) and 1 non-Pseudoaltermonas strain belonging to Alteromonas sp. (37 strains from 6 genera tested), which helps bound the phylogenetic distance possible in a phage-mediated horizontal gene transfer event. The Pseudoalteromonas phage B8b genome size was 42.7 kb, with clear structural and replication modules where the former were delineated leveraging identification of 16 structural genes by virion structural proteomics, only 4 of which had any similarity to known structural proteins. In nature, this phage was common in coastal marine environments in both photic and aphotic layers (found in 26.5% of available viral metagenomes), but not abundant in any sample (average per sample abundance was 0.65% of the reads). Together these data improve our understanding of siphoviruses in nature, and provide foundational information for a new 'rare virosphere' phage-host model system.« less

  12. Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea

    SciTech Connect

    Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; Sà, Elisabet Laia; Ignacio-Espinoza, J. Cesar; Cornejo-Castillo, Francisco M.; Verberkmoes, Nathan C.; Vaqué, Dolors; Sullivan, Matthew B.; Acinas, Silvia G.; Kellogg, Christina A.

    2015-01-14

    Marine viruses (phages) alter bacterial diversity and evolution with impacts on marine biogeochemical cycles, and yet few well-developed model systems limit opportunities for hypothesis testing. We isolate phage B8b from the Mediterranean Sea using Pseudoalteromonas sp. QC-44 as a host and characterize it using myriad techniques. Morphologically, phage B8b was classified as a member of the Siphoviridae family. One-step growth analyses showed that this siphovirus had a latent period of 70 min and released 172 new viral particles per cell. In the host range analysis against 89 bacterial host strains revealed that phage B8b infected 3 Pseudoalteromonas strains (52 tested, >99.9% 16S rRNA gene nucleotide identity) and 1 non-Pseudoaltermonas strain belonging to Alteromonas sp. (37 strains from 6 genera tested), which helps bound the phylogenetic distance possible in a phage-mediated horizontal gene transfer event. The Pseudoalteromonas phage B8b genome size was 42.7 kb, with clear structural and replication modules where the former were delineated leveraging identification of 16 structural genes by virion structural proteomics, only 4 of which had any similarity to known structural proteins. In nature, this phage was common in coastal marine environments in both photic and aphotic layers (found in 26.5% of available viral metagenomes), but not abundant in any sample (average per sample abundance was 0.65% of the reads). Together these data improve our understanding of siphoviruses in nature, and provide foundational information for a new 'rare virosphere' phage-host model system.

  13. Range 8C Rehabilitation Demonstration Project, Hohenfels Training Area, Germany: Final report

    SciTech Connect

    Zellmer, S.D.; Hinchman, R.R.; Johnson, D.O. . Energy Systems Div.); Severinghaus, W.D. ); Brent, J.J. )

    1991-11-01

    More than 30 years of intensive and continual tactical training has caused extensive environmental damage at the US Army Hohenfels Training Area in Germany. The Range 8C Rehabilitation Demonstration Project, followed by a three-year monitoring effort, was conducted to develop and evaluate the environmental and economic effectiveness of seven revegetation and four erosion control prescriptions implemented at a 16-ha site. The point-intercept method was used to measure the types and amounts of vegetation established and the changes in the vegetative community during three years of military use on the seven areas treated with revegetation prescriptions. Field observations were made to determine the suitability and durability of four types of erosion control structures. Soil fertility and a source of seed appeared to be the most limiting factors in establishing vegetation, while seedbed preparation had only a minor influence. Grasses appeared to be more resistant to vehicle traffic than did other types of vegetation. Because grassed waterways were used as roads by military vehicles and a system of graded terraces was expensive, these erosion control prescriptions were unsuitable and uneconomical for use on training areas. Low-cost riprap waterbars and porous check dams slowed the velocity of runoff, trapped sediments, and were durable. Recommendations were formulated to improve the environmental and economic effectiveness of future rehabilitation efforts on tactical training areas.

  14. Molecular cloning and sequencing of the banded dogfish (Triakis scyllia) interleukin-8 cDNA.

    PubMed

    Inoue, Yuuki; Haruta, Chiaki; Usui, Kazushige; Moritomo, Tadaaki; Nakanishi, Teruyuki

    2003-03-01

    The dogfish (Triakis scyllia) interleukin-8 (IL-8) cDNA was isolated from mitogen-stimulated peripheral white blood cells (WBCs) utilising the polymerase chain reaction (PCR). The cDNA sequence showed that the dogfish IL-8 clones contained an open reading frame encoding 101 amino acids. A short 5' untranslated region (UTR) of 70 nucleotides and a long 3' UTR of 893 nucleotides were also present in this 1.2-kb cDNA. Furthermore, the 3' UTR of the mRNA contained the AUUUA sequence that has been implicated in shortening of the half-life of several cytokines and growth factors. The predicted IL-8 peptide had one potential N-linked glycosylation site (Asn-72-Thr-74) that is not conserved in other vertebrates. It also contained four cysteine residues (Cys-34, 36, 61 and 77), which are characteristic of CXC subfamily cytokines and found in all vertebrates, to date. The dogfish IL-8 lacked an ELR motif as found in the lamprey and trout. Comparison of the deduced amino acids showed that the dogfish IL-8 sequence shared 50.5, 41.2, 37.1 and 40.4-45.5% identity with the chicken, lamprey, trout and mammalian IL-8 sequences, respectively.

  15. Improving a recombinant Zymomonas mobilis strain 8b through continuous adaptation on dilute acid pretreated corn stover hydrolysate

    DOE PAGES

    Mohagheghi, Ali; Linger, Jeffrey G.; Yang, Shihui; ...

    2015-03-31

    Complete conversion of the major sugars of biomass including both the C5 and C6 sugars is critical for biofuel production processes. Several inhibitory compounds like acetate, hydroxymethylfurfural (HMF), and furfural are produced from the biomass pretreatment process leading to ‘hydrolysate toxicity,’ a major problem for microorganisms to achieve complete sugar utilization. Therefore, development of more robust microorganisms to utilize the sugars released from biomass under toxic environment is critical. In this study, we use continuous culture methodologies to evolve and adapt the ethanologenic bacterium Zymomonas mobilis to improve its ethanol productivity using corn stover hydrolysate. The results are the following:more » A turbidostat was used to adapt the Z. mobilis strain 8b in the pretreated corn stover liquor. The adaptation was initiated using pure sugar (glucose and xylose) followed by feeding neutralized liquor at different dilution rates. Once the turbidostat reached 60% liquor content, the cells began washing out and the adaptation was stopped. Several ‘sub-strains’ were isolated, and one of them, SS3 (sub-strain 3), had 59% higher xylose utilization than the parent strain 8b when evaluated on 55% neutralized PCS (pretreated corn stover) liquor. Using saccharified PCS slurry generated by enzymatic hydrolysis from 25% solids loading, SS3 generated an ethanol yield of 75.5% compared to 64% for parent strain 8b. Furthermore, the total xylose utilization was 57.7% for SS3 versus 27.4% for strain 8b. To determine the underlying genotypes in these new sub-strains, we conducted genomic resequencing and identified numerous single-nucleotide mutations (SNPs) that had arisen in SS3. We further performed quantitative reverse transcription PCR (qRT-PCR) on genes potentially affected by these SNPs and identified significant down-regulation of two genes, ZMO0153 and ZMO0776, in SS3 suggesting potential genetic mechanisms behind SS3’s improved

  16. 13 CFR 121.410 - What are the size standards for SBA's Section 8(d) Subcontracting Program?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What are the size standards for SBA's Section 8(d) Subcontracting Program? 121.410 Section 121.410 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and...

  17. Polymorphisms in ABCB11 and ATP8B1 Associated with Development of Severe Intrahepatic Cholestasis in Hodgkin's Lymphoma.

    PubMed

    Blackmore, Laura; Knisely, A S; Hartley, Jane L; McKay, Kirsten; Gissen, Paul; Marcus, Robert; Shawcross, Debbie L

    2013-06-01

    We report a young man presenting with jaundice and severe debilitating intrahepatic cholestasis 7 months before the diagnosis of Hodgkin's lymphoma. Serum gamma-glutamyl transferase (GGT) activity was not raised. Liver biopsy demonstrated deficiency of canalicular GGT and bile salt export pump expression, which suggested "benign" recurrent intrahepatic cholestasis. Direct sequencing of genomic DNA was therefore undertaken to look for mutations in ATP8B1 and ABCB11. Cholestasis and pruritus are well recognized presenting features of Hodgkin's lymphoma. However, striking in this case is that the intrahepatic cholestasis presented and resolved 7 months before the diagnosis. Furthermore, 4 polymorphisms were identified in ATP8B1 in this patient-c.696T > C (rs319438), c.811A > C (rs319438), c.2855G > A (rs1296811) and c.3454G > A (rs222581)-and two polymorphisms in ABCB11-c.1331T > C (rs2287622) and c.3084A > G (rs497692); 2 of which have been associated with intrahepatic cholestasis of pregnancy. We therefore postulate that these polymorphisms predisposed this patient to the development of intrahepatic cholestasis within the abnormal pro-inflammatory cytokine milieu typical for Hodgkin's lymphoma. This case shows for the first time that some polymorphisms in ABCB11 and ATP8B1 may predispose to the development of intrahepatic cholestasis in Hodgkin's lymphoma. It also demonstrates the importance of close clinical surveillance for the development of Hodgkin's lymphoma in patients presenting with unexplained intrahepatic cholestasis.

  18. A 727 airplane center duct inlet low speed performance confirmation model test for refanned JT8D engines, phase 2

    NASA Technical Reports Server (NTRS)

    Kaldschmidt, G.; Syltebo, B. E.; Ting, C. T.

    1973-01-01

    The results from testing of a 0.3 scale model center duct inlet (S duct) for the Pratt and Whitney Aircraft JT8D-100 engines are presented. The objective of this test was to demonstrate that the required airflow of the JT8D-100 engine (480 lb/sec as compared to 334 lb/sec for JT8D-15) can be achieved with minimum modifications to the existing 727 airplane structure at acceptable levels of total pressure recovery and distortion. Steady-state pressure recovery, steady-state pressure distortion, and dynamic pressure measurements were taken at the engine face station. Surface static pressure measurements were taken along the duct. Test results indicated that the required airflow was achieved with acceptable pressure recovery (comparable to the current 727-200 S duct). Inlet inflow angle variation within the 727 airplane operating regime (minus 5 to 5 degrees) had no effect on the inlet performance. Pressure distortion at static and forward speed at takeoff airflow conditions are within P and WA limits for the Phase II duct when equipped with vortex generators. Static crosswind operation between 10 knots and 25 knots appears feasible at full takeoff power.

  19. Measurement of the total active 8B solar neutrino flux at the Sudbury Neutrino Observatory with enhanced neutral current sensitivity.

    PubMed

    Ahmed, S N; Anthony, A E; Beier, E W; Bellerive, A; Biller, S D; Boger, J; Boulay, M G; Bowler, M G; Bowles, T J; Brice, S J; Bullard, T V; Chan, Y D; Chen, M; Chen, X; Cleveland, B T; Cox, G A; Dai, X; Dalnoki-Veress, F; Doe, P J; Dosanjh, R S; Doucas, G; Dragowsky, M R; Duba, C A; Duncan, F A; Dunford, M; Dunmore, J A; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Formaggio, J A; Fowler, M M; Frame, K; Fulsom, B G; Gagnon, N; Graham, K; Grant, D R; Hahn, R L; Hall, J C; Hallin, A L; Hallman, E D; Hamer, A S; Handler, W B; Hargrove, C K; Harvey, P J; Hazama, R; Heeger, K M; Heintzelman, W J; Heise, J; Helmer, R L; Hemingway, R J; Hime, A; Howe, M A; Jagam, P; Jelley, N A; Klein, J R; Kos, M S; Krumins, A V; Kutter, T; Kyba, C C M; Labranche, H; Lange, R; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Levine, I; Luoma, S; MacLellan, R; Majerus, S; Mak, H B; Maneira, J; Marino, A D; McCauley, N; McDonald, A B; McGee, S; McGregor, G; Mifflin, C; Miknaitis, K K S; Miller, G G; Moffat, B A; Nally, C W; Nickel, B G; Noble, A J; Norman, E B; Oblath, N S; Okada, C E; Ollerhead, R W; Orrell, J L; Oser, S M; Ouellet, C; Peeters, S J M; Poon, A W P; Robertson, B C; Robertson, R G H; Rollin, E; Rosendahl, S S E; Rusu, V L; Schwendener, M H; Simard, O; Simpson, J J; Sims, C J; Sinclair, D; Skensved, P; Smith, M W E; Starinsky, N; Stokstad, R G; Stonehill, L C; Tafirout, R; Takeuchi, Y; Tesić, G; Thomson, M; Thorman, M; Van Berg, R; Van de Water, R G; Virtue, C J; Wall, B L; Waller, D; Waltham, C E; Tseung, H Wan Chan; Wark, D L; West, N; Wilhelmy, J B; Wilkerson, J F; Wilson, J R; Wouters, J M; Yeh, M; Zuber, K

    2004-05-07

    The Sudbury Neutrino Observatory has precisely determined the total active (nu(x)) 8B solar neutrino flux without assumptions about the energy dependence of the nu(e) survival probability. The measurements were made with dissolved NaCl in heavy water to enhance the sensitivity and signature for neutral-current interactions. The flux is found to be 5.21 +/- 0.27(stat)+/-0.38(syst) x 10(6) cm(-2) s(-1), in agreement with previous measurements and standard solar models. A global analysis of these and other solar and reactor neutrino results yields Deltam(2)=7.1(+1.2)(-0.6) x 10(-5) eV(2) and theta=32.5(+2.4)(-2.3) degrees. Maximal mixing is rejected at the equivalent of 5.4 standard deviations.

  20. Parametric Study of a YAV-8B Harrier in Ground Effect using Time-Dependent Navier-Stokes Computations

    NASA Technical Reports Server (NTRS)

    Pandya, Shishir; Chaderjian, Neal; Ahmad, Jasim; Kwak, Dochan (Technical Monitor)

    2002-01-01

    A process is described which enables the generation of 35 time-dependent viscous solutions for a YAV-8B Harrier in ground effect in one week. Overset grids are used to model the complex geometry of the Harrier aircraft and the interaction of its jets with the ground plane and low-speed ambient flow. The time required to complete this parametric study is drastically reduced through the use of process automation, modern computational platforms, and parallel computing. Moreover, a dual-time-stepping algorithm is described which improves solution robustness. Unsteady flow visualization and a frequency domain analysis are also used to identify and correlated key flow structures with the time variation of lift.

  1. Heterologous Expression of ATG8c from Soybean Confers Tolerance to Nitrogen Deficiency and Increases Yield in Arabidopsis

    PubMed Central

    Liu, Dong; Chai, Wenting; Gong, Qingqiu; Wang, Ning Ning

    2012-01-01

    Nitrogen is an essential element for plant growth and yield. Improving Nitrogen Use Efficiency (NUE) of crops could potentially reduce the application of chemical fertilizer and alleviate environmental damage. To identify new NUE genes is therefore an important task in molecular breeding. Macroautophagy (autophagy) is an intracellular process in which damaged or obsolete cytoplasmic components are encapsulated in double membraned vesicles termed autophagosomes, then delivered to the vacuole for degradation and nutrient recycling. One of the core components of autophagosome formation, ATG8, has been shown to directly mediate autophagosome expansion, and the transcript of which is highly inducible upon starvation. Therefore, we postulated that certain homologs of Saccharomyces cerevisiae ATG8 (ScATG8) from crop species could have potential for NUE crop breeding. A soybean (Glycine max, cv. Zhonghuang-13) ATG8, GmATG8c, was selected from the 11 family members based on transcript analysis upon nitrogen deprivation. GmATG8c could partially complement the yeast atg8 mutant. Constitutive expression of GmATG8c in soybean callus cells not only enhanced nitrogen starvation tolerance of the cells but accelerated the growth of the calli. Transgenic Arabidopsis over-expressing GmATG8c performed better under extended nitrogen and carbon starvation conditions. Meanwhile, under optimum growth conditions, the transgenic plants grew faster, bolted earlier, produced larger primary and axillary inflorescences, eventually produced more seeds than the wild-type. In average, the yield was improved by 12.9%. We conclude that GmATG8c may serve as an excellent candidate for breeding crops with enhanced NUE and better yield. PMID:22629371

  2. Screening a phage display library for a novel FGF8b-binding peptide with anti-tumor effect on prostate cancer

    SciTech Connect

    Wang, Wenhui; Chen, Xilei; Li, Tao; Li, Yanmei; Wang, Ruixue; He, Dan; Luo, Wu; Li, Xiaokun; Wu, Xiaoping

    2013-05-01

    Fibroblast growth factor 8b (FGF8b) is the major isoform of FGF8 expressed in prostate cancer and it correlates with the stage and grade of the disease. FGF8b has been considered as a potential target for prostate cancer therapy. Here we isolated 12 specific FGF8b-binding phage clones by screening a phage display heptapeptide library with FGF8b. The peptide (HSQAAVP, named as P12) corresponding to one of these clones showed high homology to the immunoglobulin-like (Ig-like) domain II(D2) of high-affinity FGF8b receptor (FGFR3c), contained 3 identical amino acids (AVP) to the authentic FGFR3 D2 sequence aa 163–169 (LLAVPAA) directly participating in ligand binding, carried the same charges as its corresponding motif (aa163–169) in FGFR3c, suggesting that P12 may have a greater potential to interrupt FGF8b binding to its receptors than other identified heptapeptides do. Functional analysis indicated that synthetic P12 peptides mediate significant inhibition of FGF8b-induced cell proliferation, arrest cell cycle at the G0/G1 phase via suppression of Cyclin D1 and PCNA, and blockade of the activations of Erk1/2 and Akt cascades in both prostate cancer cells and vascular endothelial cells. The results demonstrated that the P12 peptide acting as an FGF8b antagonist may have therapeutic potential in prostate cancer. - Highlights: ► A novel FGF8b-binding peptide P12 was isolated from a phage display library. ► The mechanisms for P12 peptide inhibiting cell proliferation were proposed. ► P12 caused cell cycle arrest at G0/G1 phase via suppression of Cyclin D1 and PCNA. ► P12 suppressed FGF8b-induced activations of Akt and MAP kinases. ► P12 acting as an FGF8b antagonist may have therapeutic potential in prostate cancer.

  3. 29 CFR 101.23 - Initiation and investigation of a petition in connection with a case under section 8(b)(7).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... employer for recognition or that the union represents a substantial number of employees. (1) The employer's... for an object proscribed by section 8(b)(7) of the Act; (3) Subparagraph (C) of that section of...

  4. 26 CFR 31.3401(a)(8)(B)-1 - Remuneration for services performed in possession of the United States (other than Puerto Rico...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... calendar year will be for such services. The reasonable belief contemplated by section 3401(a)(8)(B) may be based upon any evidence reasonably sufficient to induce such belief, even though such evidence may...

  5. Phase 2 program on ground test of refanned JT8D turbofan engines and nacelles for the 727 airplane. Volume 3: Ground tests

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The NASA Refan Program included full-scale performance and noise ground tests of both a current production (JT8D-15) and a refanned (JT8D-115) engine. A description of the two ground tests including detailed propulsion, noise, and structural test results is presented. The primary objectives of the total test program were comparison of JT8D-15 and JT8D-115 overall propulsion system performance and noise characteristics and determination of incremental component noise levels. Other objectives of the test program included: (1) determination of acoustic treatment effectiveness; (2) measurement of internal sound pressure levels; (3) measurement of inlet and exhaust hardware performance; (4) determination of center-engine surge margin; and (5) evaluation of certain structural characteristics associated with the 727 refan center-engine inlet duct and JT8D refan engine exhaust system. The JT8D-15 and -115 tests were conducted during September 1974 and January to March 1975, respectively. Analyses of the test data indicated that the JT8D-115, as compared to the JT8D-15, demonstrates a 12.5 percent to 13.2 percent reduction in static specific fuel consumption, and a reduction of 6 to 7 PNdB in a weighted average value of static tone corrected perceived noise level. Separated into noise components, a significant reduction was shown for the inlet fan, aft fan, exhaust duct flow, turbine, and jet noises. However, core noise was increased. Photographs of test stands and test equipment are shown.

  6. Design and development experience with a digital fly-by-wire control system in an F-8C airplane

    NASA Technical Reports Server (NTRS)

    Deets, D. A.

    1976-01-01

    To assess the feasibility of a digital fly by wire system, the mechanical flight control system of an F-8C airplane was replaced with a digital primary system and an analog backup system. The Apollo computer was used as the heart of the primary system. This paper discusses the experience gained during the design and development of the system and relates it to active control systems that are anticipated for future civil transport applications.

  7. The stochastic control of the F-8C aircraft using the Multiple Model Adaptive Control (MMAC) method

    NASA Technical Reports Server (NTRS)

    Athans, M.; Dunn, K. P.; Greene, E. S.; Lee, W. H.; Sandel, N. R., Jr.

    1975-01-01

    The purpose of this paper is to summarize results obtained for the adaptive control of the F-8C aircraft using the so-called Multiple Model Adaptive Control method. The discussion includes the selection of the performance criteria for both the lateral and the longitudinal dynamics, the design of the Kalman filters for different flight conditions, the 'identification' aspects of the design using hypothesis testing ideas, and the performance of the closed loop adaptive system.

  8. Design and development experience with a digital fly-by-wire control system in an F-8C airplane

    NASA Technical Reports Server (NTRS)

    Deets, D. A.

    1975-01-01

    To assess the feasibility of a digital fly-by-wire system, the mechanical flight control system of an F-8C airplane was replaced with a digital system and an analog backup system. The Apollo computer was used as the heart of the primary system. This paper discusses the experience gained during the design and development of the system and relates it to active control systems that are anticipated for future civil transport applications.

  9. The 727 airplane side inlet low-speed performance confirmation model test for refanned JT8D engines

    NASA Technical Reports Server (NTRS)

    Schuehle, A. L.

    1974-01-01

    The results of a low-speed wind tunnel test of a 0.3 scale model 727 airplane side inlet for JT8D-100 engines are presented. The objectives of the test were to develop lines for a full-scale flightworthy inlet, to evaluate inlet total pressure recovery and steady-state total pressure distortion, and to obtain model-scale distortion data which can be used in the assessment of the compatibility of the inlet with the JT8D-100 series engines. A secondary objective was to obtain internal/external cowl static pressures for the determination of nacelle loads. Two basic inlet models were tested at static, forward speed, angle-of-attack (inflow angle), and cross-wind conditions. One model was with and one without an acoustic ring. Two modifications to the models were also tested, one with the ring closer to the inlet throat and one with a larger lip. Test measurements consisted of inlet surface static pressure, engine face total pressure, inlet airflow, tunnel total pressure, tunnel total temperature and tunnel velocity. Total pressure traverses were taken directly behind the ring and strut. No dynamic measurements were taken.

  10. Thermal and magnetic properties of amorphous Prx(Fe0.8B0.2)1 - x

    NASA Astrophysics Data System (ADS)

    Kabacoff, L.; Dallek, S.; Modzelewski, C.; Krull, W.

    1982-03-01

    Amorphous ribbons of the composition Prx(Fe0.8B0.2)1-, 0?x?0.30, have been produced by meltspinning onto a copper wheel under argon atmosphere. The alloys were characterized thermally using a DuPont 1090 Thermal Analysis System. The crystallization onset temperature Tx, measured at a heating rate of 20 K/min, has a maximum of 899 K at x = 0.10. Tx equals 696 and 729 K for x = 0 and x = 0.30, respectively. The activation energy of crystallization of 5.4 eV for x = 0.05, dropping to 4.3 eV at x = 0.10, remaining approximately constant betwen x = 0.10 and 0.20, then dropping to 2.7 eV for x = 0.30. There is evidence that the alloys phase-separate slowly at room temperature, rapidly at elevated temperature. The magnetic moment was measured as a function of applied field at room temperature and at 4.2 K. The saturation moment decreased from 142 to 50.2 emu/gm at room temperature, and from 171.5 to 93.5 emu/gm at 4.2 K. The low temperature moments were strongly time dependent. The room temperature coercive force was too small to measure (<5 Oe).

  11. Postprandial response and tissue distribution of the bile acid synthesis-related genes, cyp7a1, cyp8b1 and shp, in rainbow trout Oncorhynchus mykiss.

    PubMed

    Murashita, Koji; Yoshiura, Yasutoshi; Chisada, Shin-ichi; Furuita, Hirofumi; Sugita, Tsuyoshi; Matsunari, Hiroyuki; Yamamoto, Takeshi

    2013-10-01

    In mammals, cholesterol 7α-hydroxylase (CYP7A1) and sterol 12α-hydroxylase (CYP8B1) are rate-limiting enzymes in bile acid synthesis. In addition, a small heterodimer partner (SHP) is also known to inhibit bile acid synthesis via the suppression of CYP7A1 and CYP8B1 expression. However, little information is currently available regarding primary structure of the genes involved in bile acid synthesis in fish. We therefore cloned cyp7a1, cyp8b1 and shp genes from rainbow trout and obtained cDNAs encoding two isoforms each of Cyp7a1 (-1 and -2), Cyp8b1 (-1 and -2) and Shp (-1 and -2). Both cyp7a1-1 and -2 encoded proteins of 512 amino acids. Trout cyp7a1-1 was expressed not only primarily in the kidney, pyloric caecum and mid-gut, but also weakly in the liver, eye, gill and ovary. cyp7a1-2 was highly expressed in the liver, pyloric caecum and mid-gut. cyp8b1-1 and -2, which encoded proteins of 512 and 509 amino acids, respectively, were principally expressed in the liver. Both shp-1 and -2, which encoded proteins of 288 and 290 amino acids, respectively, were strongly expressed in the liver, but shp-2 was also highly expressed in the gallbladder and digestive tract. The temporal changes in the expression of cyp7a1-1/-2, cyp8b1-1/-2 and shp-1/-2 in the liver were assessed after consumption of a single meal. Expression of cyp7a1-1/-2 and cyp8b1-1/-2 increased within 3h post feeding (hpf) when the stomach was still approximately 84% full and the gallbladder was almost completely empty. Although the expression of shp-1 did not change after feeding, the expression pattern of shp-2 was inversely related to the expression patterns of cyp7a1-1/-2 and cyp8b1-1/-2. Specifically, shp-2 expression decreased until 3 hpf before returning to initial levels at 24 hpf. These findings suggest that Cyp7a1s/8b1s and Shp-2 function antagonistically in bile acid synthesis in rainbow trout.

  12. Dynamic regulation of cardiolipin by the lipid pump Atp8b1 determines the severity of lung injury in experimental pneumonia.

    PubMed

    Ray, Nancy B; Durairaj, Lakshmi; Chen, Bill B; McVerry, Bryan J; Ryan, Alan J; Donahoe, Michael; Waltenbaugh, Alisa K; O'Donnell, Christopher P; Henderson, Florita C; Etscheidt, Christopher A; McCoy, Diann M; Agassandian, Marianna; Hayes-Rowan, Emily C; Coon, Tiffany A; Butler, Phillip L; Gakhar, Lokesh; Mathur, Satya N; Sieren, Jessica C; Tyurina, Yulia Y; Kagan, Valerian E; McLennan, Geoffrey; Mallampalli, Rama K

    2010-10-01

    Pneumonia remains the leading cause of death from infection in the US, yet fundamentally new conceptual models underlying its pathogenesis have not emerged. We show that humans and mice with bacterial pneumonia have markedly elevated amounts of cardiolipin, a rare, mitochondrial-specific phospholipid, in lung fluid and find that it potently disrupts surfactant function. Intratracheal cardiolipin administration in mice recapitulates the clinical phenotype of pneumonia, including impaired lung mechanics, modulation of cell survival and cytokine networks and lung consolidation. We have identified and characterized the activity of a unique cardiolipin transporter, the P-type ATPase transmembrane lipid pump Atp8b1, a mutant version of which is associated with severe pneumonia in humans and mice. Atp8b1 bound and internalized cardiolipin from extracellular fluid via a basic residue-enriched motif. Administration of a peptide encompassing the cardiolipin binding motif or Atp8b1 gene transfer in mice lessened bacteria-induced lung injury and improved survival. The results unveil a new paradigm whereby Atp8b1 is a cardiolipin importer whose capacity to remove cardiolipin from lung fluid is exceeded during inflammation or when Atp8b1 is defective. This discovery opens the door for new therapeutic strategies directed at modulating the abundance or molecular interactions of cardiolipin in pneumonia.

  13. Determination of astrophysical 7Be(p, γ)8B reaction rates from the 7Li(d, p)8Li reaction

    NASA Astrophysics Data System (ADS)

    Du, XianChao; Guo, Bing; Li, ZhiHong; Pang, DanYang; Li, ErTao; Liu, WeiPing

    2015-06-01

    The 7Be(p, γ)8B reaction plays a central role not only in the evaluation of solar neutrino fluxes but also in the evolution of the first stars. Study of this reaction requires the asymptotic normalization coefficient (ANC) for the virtual decay 8B g.s. → 7Be + p. By using the charge symmetry relation, we obtain this proton ANC with the single neutron ANC of 8Li g.s. →7Li + n, which is determined with the distorted wave Born approximation (DWBA) and adiabatic distorted wave approximation (ADWA) analysis of the 7Li(d, p)8Li angular distribution. The astrophysical S-factors and reaction rates of the direct capture process in the 7Be(p, γ)8B reaction are further deduced at energies of astrophysical relevance. The astrophysical S-factor at zero energy for direct capture, S 17(0), is derived to be (19.9 ± 3.5) eV b in good agreement with the most recent recommended value. The contributions of the 1+ and 3+ resonances to the S-factor and reaction rate are also evaluated. The present result demonstrates that the direct capture dominates the 7Be(p, γ)8B reaction in the whole temperature range. This work provides an independent examination to the current results of the 7Be(p, γ)8B reaction.

  14. {sup 7}Be(p,{gamma}){sup 8}B S factor from ab initio no-core shell model wave functions

    SciTech Connect

    Navratil, P.; Bertulani, C.A.; Caurier, E.

    2006-06-15

    Nuclear structure of {sup 7}Be, {sup 8}B, and {sup 7,8}Li is studied within the ab initio no-core shell model (NCSM). Starting from high-precision nucleon-nucleon (NN) interactions, wave functions of {sup 7}Be and {sup 8}B bound states are obtained in basis spaces up to 10({Dirac_h}/2{pi}){omega} and used to calculate channel cluster form factors (overlap integrals) of the {sup 8}B ground state with {sup 7}Be+p. Due to the use of the harmonic oscillator (HO) basis, the overlap integrals have incorrect asymptotic properties. We fix this problem in two alternative ways. First, by a Woods-Saxon potential solution fit to the interior of the NCSM overlap integrals. Second, by a direct matching with the Whittaker function. The corrected overlap integrals are then used for the {sup 7}Be(p,{gamma}){sup 8}B S-factor calculation. We study the convergence of the S factor with respect to the NCSM HO frequency and the model space size. Our S factor results agree with recent direct measurement data. We also test the spectroscopic factors and the corrected overlap integrals from the NCSM in describing the momentum distributions in knockout reactions with {sup 8}B projectiles. A good agreement with the available experimental data is also found, attesting to the overall consistency of the calculations.

  15. Table-like magnetocaloric effect of Fe88-xNdxCr8B4 composite materials

    NASA Astrophysics Data System (ADS)

    Lai, J. W.; Zheng, Z. G.; Zhong, X. C.; Franco, V.; Montemayor, R.; Liu, Z. W.; Zeng, D. C.

    2015-09-01

    The narrow working temperature range due to the sharp magnetic entropy change |ΔSM| peak and large thermal or magnetic hysteresis restricts the practical application of magnetocaloric materials. In this work, the table-like magnetocaloric effect (MCE) was obtained in the multilayer composite of Fe88-xNdxCr8B4 alloys with various Nd substitutions for Fe (x=5, 8, 10, 12, and 15), which were prepared by arc-melting followed by melt-spinning. The substation of Nd was found to enhance the glass-forming ability. For the alloys with Nd substitution from 5 at% to 15 at%, the Curie temperature (TC) ranged from 322 K to 350 K and the peak value of |ΔSM| remained almost constant, 3.4-3.5 J/(kg K) under an applied field of 0-5 T. The composite with various Nd contents was prepared by stocking the ribbons layer by layer. The |ΔSM| of the composite approached a nearly constant value of ˜3.2 J/(kg K) in a field change of 0-5 T in a wide temperature span over 40 K, resulting in large refrigerant capacity value of >408 J/kg. This |ΔSM| value was much larger than the previous reported Fe-based amorphous composite Fe78-xCexSi4Nb5B12Cu1. This composite can be used as the working material in the Ericsson-cycle magnetic regenerative refrigerator around room temperature.

  16. Nitrogen redistribution and its relationship with the expression of GmATG8c during seed filling in soybean.

    PubMed

    Islam, Md Matiul; Ishibashi, Yushi; Nakagawa, Andressa C S; Tomita, Yuki; Iwaya-Inoue, Mari; Arima, Susumu; Zheng, Shao-Hui

    2016-03-15

    It is well known that some nitrogen in the vegetative organs is redistributed to the seeds during seed filling in soybean (Glycine max [L.] Merrill). This redistribution is considered to affect the seed yield of soybean. However, it is still not clear when the nitrogen moves from the vegetative part to the seeds, and the relationship between nitrogen redistribution and leaf senescence has not been clarified. The soybean variety Fukuyutaka was grown in the experimental field of Saga University, Japan from 22 July to 31 October, 2014. After the first flower stage (R1), the plant samples were collected weekly and were separated into leaf, petiole, stem, podshell and seed. The nitrogen concentrations in each plant part were determined. Fresh leaf samples were provided for the determination of soluble protein and autophagy gene GmATG8c expression. The nitrogen that accumulated in the vegetative parts reached its highest level at 60days after sowing (DAS), then began to decrease at 73DAS (R6). This decrease is considered to be the consequence of nitrogen redistribution from the vegetative parts to the seeds. The movement of nitrogen from the vegetative parts to the seeds was estimated to occur at around 73DAS (R6). At this stage, leaf SPAD values, leaf nitrogen, and soluble protein concentrations began to decrease simultaneously, suggesting the onset of leaf senescence. Furthermore, the expression of the autophagy gene GmATG8c in the leaves increased dramatically from 73 to 85DAS, which is the duration of nitrogen redistribution. The results suggest that the nitrogen redistribution from the vegetative parts to the seeds could be one of the initiating factors of leaf senescence, and the autophagy gene GmATG8c was associated with this process.

  17. Differential hepatocellular zonation pattern of cholesterol 7alpha-hydroxylase (Cyp7a1) and sterol 12alpha-hydroxylase (Cyp8b1) in the mouse.

    PubMed

    Wang, Jin; Olin, Maria; Rozell, Björn; Björkhem, Ingemar; Einarsson, Curt; Eggertsen, Gösta; Gåfvels, Mats

    2007-03-01

    The synthesis of primary bile acids is confined to the hepatocytes. This study aimed to evaluate the expression pattern within the liver architecture of the rate-limiting enzyme of the neutral pathway, cholesterol 7alpha-hydroxylase (Cyp7a1), and sterol 12alpha-hydroxylase (Cyp8b1), the enzyme necessary for the synthesis of cholic acid. Specific Cyp8b1 and Cyp7a1 peptide antiserums were used for immunohistochemical staining of livers from wild type and Cyp8b1 null mice, the latter instead expressing beta-galactosidase (beta-Gal) as a replacement reporter gene. Cyp8b1 was mainly expressed in the hepatocytes in a zonal pattern surrounding the central vein while the areas surrounding the portal zones showed much lower levels. The zonation was maintained in cholic acid-depleted mice using beta-Gal as a reporter protein. Cyp7a1 expression in wild type mice also showed a zonal distribution pattern, although less distinct, with a maximal expression within a 1-2 cell thick layer of hepatocytes surrounding the central vein. In Cyp8b1 null mice, a more intense staining was obtained, in accordance with the higher expression level of Cyp7a1, although the overall expression pattern was maintained. Our results in mice indicate possible differences in the regulation of the cellular zonation of Cyp7a1 and Cyp8b1. Also, cholic acid affects the set-point of Cyp7a1 expression but not its zonal distribution.

  18. Parametric Time-Dependent Navier-Stokes Computations for a YAV-8B Harrier in Ground Effect

    NASA Technical Reports Server (NTRS)

    Chaderjian, Neal M.; Pandya, Shishir; Ahmad, Jasim; Murman, Scott; Kwak, Dochan (Technical Monitor)

    2002-01-01

    The Harrier Jump Jet has the distinction of being the only powered-lift aircraft in the free world to achieve operational status and to have flown in combat. This V/STOL aircraft can take-off and land vertically or utilize very short runways by directing its four exhaust nozzles towards the ground. Transition to forward flight is achieved by rotating these nozzles into a horizontal position. Powered-lift vehicles have certain advantages over conventional strike fighters. Their V/STOL capabilities allow for safer carrier operations, smaller carrier size, and quick reaction time for troop support. Moreover, they are not dependent on vulnerable land-based runways. The AV-8A Harrier first entered service in the British Royal Air Force (RAF) during 1969, and the U.S. Marine Corps (USMC) in 1971. The AV-8B was a redesign to achieve improved payload capacity, range, and accuracy. This modified design first entered service with the USMC and RAF in 1985. The success and unique capabilities of the Harrier has prompted the design of a powered-lift version of the Joint Strike Fighter (JSF). The flowfield for the Harrier near the ground during low-speed or hover flight operations is very complex and time-dependent. A sketch of this flowfield is shown. Warm air from the fan is exhausted from the front nozzles, while a hot air/fuel mixture from the engine is exhausted from the rear nozzles. These jets strike the ground and move out radially forming a ground jet-flow. The ambient freestream, due to low-speed forward flight or - headwind during hover, opposes the jet-flow. This interaction causes the flow to separate and form a ground vortex. The multiple jets also interact with each other near the ground and form an upwash or jet fountain, which strikes the underside of the fuselage. If the aircraft is sufficiently close to the ground, the inlet can ingest ground debris and hot gases from the fountain and ground vortex. This Hot Gas Ingestion (HGI) can cause a sudden loss of

  19. PK/TD modeling for prediction of the effects of 8C2, an anti-topotecan mAb, on topotecan-induced toxicity in mice

    PubMed Central

    Shah, Dhaval K.; Balthasar, Joseph P.

    2014-01-01

    To facilitate the development of an inverse targeting strategy, where anti-topotecan antibodies are administered to prevent systemic toxicity following intraperitoneal topotecan, a pharmacokinetic/toxicodynamic (PK/TD) model was developed and evaluated. The pharmacokinetics of 8C2, a monoclonal anti-topotecan antibody, were assessed following IV and SC administration, and the data were characterized using a two compartmental model with nonlinear absorption and elimination. A hybrid PK model was constructed by combining a PBPK model for topotecan with the two-compartment model for 8C2, and the model was employed to predict the disposition of topotecan, 8C2, and the topotecan-8C2 complex. The model was linked to a toxicodynamic model for topotecan-induced weight-loss, and simulations were conducted to predict the effects of 8C2 on the toxicity of topotecan in mice. Increasing the molar dose ratio of 8C2 to topotecan resulted in a dose-dependent decrease in the unbound (i.e., not bound to 8C2) topotecan exposure in plasma (AUCf) and a decrease in the extent of topotecan-induced weight-loss. Consistent with model predictions, toxicodynamic experiments showed substantial reduction in the percent nadir weight loss observed with 30 mg/kg IP topotecan after co-administration of 8C2 (20±8% vs. 10±8%). The investigation supports the use of anti-topotecan mAb to reduce the systemic toxicity of IP topotecan chemotherapy. PMID:24508555

  20. The effects of ordered carbon vacancies on stability and thermo-mechanical properties of V8C7 compared with VC

    PubMed Central

    Chong, XiaoYu; Jiang, YeHua; Zhou, Rong; Feng, Jing

    2016-01-01

    The ordered non-stoichiometric V8C7 can form in the VCy carbides by the disorder–order phase transformation. The intrusion of ordered carbon vacancies can affect their stability, mechanical, thermal and electronic properties. The relatively thermodynamic stability and mechanical properties at high temperature for the ordered stoichiometric VC and non-stoichiometric V8C7 are investigated in this paper by first-principle calculations combined with the quasi-harmonic approximation. The difference between the properties of VC and V8C7 can be obtained. We find that the V8C7 is thermodynamic more stable than VC, but has weaker elastic heat resistance than VC. Moreover, the minimum thermal conductivity of VC is a little larger than V8C7 and a simple way is proposed to characterize the anisotropy of lattice thermal conductivity based on the Cahill’s model. PMID:27659072

  1. Titanium honeycomb acoustic lining structural and thermal test report. [for acoustic tailpipe for JT8D engine

    NASA Technical Reports Server (NTRS)

    Joynes, D.; Balut, J. P.

    1974-01-01

    The results are presented of static, fatigue and thermal testing of titanium honeycomb acoustic panels representing the acoustic tailpipe for the Pratt and Whitney Aircraft JT8D Refan engine which is being studied for use on the Boeing 727-200 airplane. Test specimens represented the engine and tailpipe flange joints, the rail to which the thrust reverser is attached and shear specimens of the tailpipe honeycomb. Specimens were made in four different batches with variations in configuration, materials and processes in each. Static strength of all test specimens exceeded the design ultimate load requirements. Fatigue test results confirmed that aluminum brazed titanium, as used in the Refan tailpipe design, meets the fatigue durability objectives. Quality of welding was found to be critical to life, with substandard welding failing prematurely, whereas welding within the process specification exceeded the panel skin life. Initial fatigue testing used short grip length bolts which failed prematurely. These were replaced with longer bolts and subsequent testing demonstrated the required life. Thermal tests indicate that perforated skin acoustic honeycomb has approximately twice the heat transfer of solid skin honeycomb.

  2. Complete Genome Sequences of Caldicellulosiruptor sp. Strain Rt8.B8, Caldicellulosiruptor sp. Strain Wai35.B1, and "Thermoanaerobacter cellulolyticus".

    PubMed

    Lee, Laura L; Izquierdo, Javier A; Blumer-Schuette, Sara E; Zurawski, Jeffrey V; Conway, Jonathan M; Cottingham, Robert W; Huntemann, Marcel; Copeland, Alex; Chen, I-Min A; Kyrpides, Nikos; Markowitz, Victor; Palaniappan, Krishnaveni; Ivanova, Natalia; Mikhailova, Natalia; Ovchinnikova, Galina; Andersen, Evan; Pati, Amrita; Stamatis, Dimitrios; Reddy, T B K; Shapiro, Nicole; Nordberg, Henrik P; Cantor, Michael N; Hua, Susan X; Woyke, Tanja; Kelly, Robert M

    2015-05-14

    The genus Caldicellulosiruptor contains extremely thermophilic, cellulolytic bacteria capable of lignocellulose deconstruction. Currently, complete genome sequences for eleven Caldicellulosiruptor species are available. Here, we report genome sequences for three additional Caldicellulosiruptor species: Rt8.B8 DSM 8990 (New Zealand), Wai35.B1 DSM 8977 (New Zealand), and "Thermoanaerobacter cellulolyticus" strain NA10 DSM 8991 (Japan).

  3. Complete Genome Sequences of Caldicellulosiruptor sp. Strain Rt8.B8, Caldicellulosiruptor sp. Strain Wai35.B1, and “Thermoanaerobacter cellulolyticus”

    PubMed Central

    Lee, Laura L.; Izquierdo, Javier A.; Blumer-Schuette, Sara E.; Zurawski, Jeffrey V.; Conway, Jonathan M.; Cottingham, Robert W.; Huntemann, Marcel; Copeland, Alex; Chen, I-Min A.; Kyrpides, Nikos; Markowitz, Victor; Palaniappan, Krishnaveni; Ivanova, Natalia; Mikhailova, Natalia; Ovchinnikova, Galina; Andersen, Evan; Pati, Amrita; Stamatis, Dimitrios; Reddy, T.B.K.; Shapiro, Nicole; Nordberg, Henrik P.; Cantor, Michael N.; Hua, Susan X.; Woyke, Tanja

    2015-01-01

    The genus Caldicellulosiruptor contains extremely thermophilic, cellulolytic bacteria capable of lignocellulose deconstruction. Currently, complete genome sequences for eleven Caldicellulosiruptor species are available. Here, we report genome sequences for three additional Caldicellulosiruptor species: Rt8.B8 DSM 8990 (New Zealand), Wai35.B1 DSM 8977 (New Zealand), and “Thermoanaerobacter cellulolyticus” strain NA10 DSM 8991 (Japan). PMID:25977428

  4. Flight-test results using nonlinear control with the F-8C digital fly-by-wire aircraft

    NASA Technical Reports Server (NTRS)

    Larson, R. R.; Smith, R. E.; Krambeer, K. D.

    1983-01-01

    The design and operation of the cooperative advanced digital research experiment (CADRE) to develop nonlinear pitch flight control algorithms is described, and the results of an in-flight evaluation using the F-8C digital fly-by-wire (DFBW) research aircraft are presented. The CADRE controller is described, including the initial filter, linear command prefilter, nonlinear command prefilter, and gain scheduling. The variable-integral control-to-optimize response of the controller is considered, and CADRE parameter combinations are addressed. The remotely-augmented-vehicle interface used in the DFBW aircraft experiment is discussed. The distanct-tracking and close-formation tracking evaluation tasks for the aircraft are described along with evaluation configurations, and the test results are presented and discussed. The latter indicate that a nonlinear adaptive controller is a feasible control system technique for the fighter tracking task.

  5. NASA/RAE collaboration on nonlinear control using the F-8C digital fly-by-wire aircraft

    NASA Technical Reports Server (NTRS)

    Butler, G. F.; Corbin, M. J.; Mepham, S.; Stewart, J. F.; Larson, R. R.

    1983-01-01

    Design procedures are reviewed for variable integral control to optimize response (VICTOR) algorithms and results of preliminary flight tests are presented. The F-8C aircraft is operated in the remotely augmented vehicle (RAV) mode, with the control laws implemented as FORTRAN programs on a ground-based computer. Pilot commands and sensor information are telemetered to the ground, where the data are processed to form surface commands which are then telemetered back to the aircraft. The RAV mode represents a singlestring (simplex) system and is therefore vulnerable to a hardover since comparison monitoring is not possible. Hence, extensive error checking is conducted on both the ground and airborne computers to prevent the development of potentially hazardous situations. Experience with the RAV monitoring and validation procedures is described.

  6. Association between chromosomal aberration of COX8C and tethered spinal cord syndrome: array-based comparative genomic hybridization analysis

    PubMed Central

    Zhao, Qiu-jiong; Bai, Shao-cong; Cheng, Cheng; Tao, Ben-zhang; Wang, Le-kai; Liang, Shuang; Yin, Ling; Hang, Xing-yi; Shang, Ai-jia

    2016-01-01

    Copy number variations have been found in patients with neural tube abnormalities. In this study, we performed genome-wide screening using high-resolution array-based comparative genomic hybridization in three children with tethered spinal cord syndrome and two healthy parents. Of eight copy number variations, four were non-polymorphic. These non-polymorphic copy number variations were associated with Angelman and Prader-Willi syndromes, and microcephaly. Gene function enrichment analysis revealed that COX8C, a gene associated with metabolic disorders of the nervous system, was located in the copy number variation region of Patient 1. Our results indicate that array-based comparative genomic hybridization can be used to diagnose tethered spinal cord syndrome. Our results may help determine the pathogenesis of tethered spinal cord syndrome and prevent occurrence of this disease. PMID:27651783

  7. Performance of Diffusion Aluminide Coatings Applied on Alloy CF8C-Plus at 800oC

    SciTech Connect

    Kumar, Deepak; Dryepondt, Sebastien N; Zhang, Ying; Haynes, James A; Pint, Bruce A; Armstrong, Beth L; Shyam, Amit; Lara-Curzio, Edgar

    2012-01-01

    High performance cast stainless steel, CF8C-Plus, is a low cost alloy with prospective applications ranging from covers and casings of small and medium size gas turbines to turbocharger housing and manifolds in internal combustion engines. Diffusion aluminide coatings were applied on this alloy as a potential strategy for improved oxidation resistance, particularly in wet air and steam. In this paper the performance of the aluminide coatings evaluated by cyclic oxidation experiments in air containing 10 vol.% H2O at 800 C and conventional tension-compression low-cycle-fatigue tests in air at 800 C with a strain range of 0.5% is presented. The results show that specimens coated by a chemical vapor deposition process provide better oxidation resistance than those coated by an Al-slurry coating process. The application of a coating by pack cementation reduced the fatigue life by 15%.

  8. Preliminary system design study for a digital fly-by-wire flight control system for an F-8C aircraft

    NASA Technical Reports Server (NTRS)

    Seacord, C. L.; Vaughn, D. K.

    1976-01-01

    The design of a fly-by-wire control system having a mission failure probability of less than one millionth failures per flight hour is examined. Emphasis was placed on developing actuator configurations that would improve the system performance, and consideration of the practical aspects of sensor/computer and computer/actuator interface implementation. Five basic configurations were defined as appropriate candidates for the F-8C research aircraft. Options on the basic configurations were included to cover variations in flight sensors, redundancy levels, data transmission techniques, processor input/output methods, and servo actuator arrangements. The study results can be applied to fly by wire systems for transport aircraft in general and the space shuttle.

  9. A reliable dual-redundant sensor FDI system for the NASA F8C-DFBW aircraft

    NASA Technical Reports Server (NTRS)

    Deckert, J. C.; Desai, M. N.; Deyst, J. J., Jr.; Willsky, A. S.

    1976-01-01

    In this paper we outline the structure of a sensor failure detection and identification (FDI) system designed for the NASA F8C DFBW aircraft. The system is for use in a dual-redundant environment, and it takes maximal advantage of all functional and kinematic relationships among the sensed variables. The technique is reliable in that we have designed the system to account for a variety of unmodeled effects and have introduced a new concept - the quality sequential probability ratio (QSPR) - that provides a useful measure of our confidence in each test. The methodology used for determining detector parameters and the 'outer loop logic' for combining the various sources of information are described.

  10. Dual redundant sensor FDI techniques applied to the NASA F8C DFBW aircraft. [Failure Detection and Identification

    NASA Technical Reports Server (NTRS)

    Desai, M. N.; Deckert, J. C.; Deyst, J. J.; Willsky, A. S.; Chow, E. Y.

    1976-01-01

    An onboard failure detection and identification (FDI) technique for dual redundant sensors on the NASA F8C digital fly-by-wire (DFBW) aircraft is presented. The failure of one of a pair of sensors of the same type is detected by a direct redundancy trigger which observes the difference between the outputs of these two sensors. Identification of the failed sensor is accomplished utilizing the analytic redundancy that exists as kinematic and functional relationships among the variables being measured by dissimilar instruments. In addition, identification of generic failures, common to both instruments of a given type, is accomplished by using a time trigger to periodically initiate analytic redundancy failure identification tests for individual sensors. The basic form of these tests is the comparison of the measurement of a variable using the suspect instrument with another measurement of the same variable obtained using other instrument types.

  11. NASA/RAE collaboration on nonlinear control using the F-8C digital fly-by-wire aircraft

    NASA Technical Reports Server (NTRS)

    Butler, G. F.; Corbin, M. J.; Mepham, S.; Stewart, J. F.; Larson, R. R.

    1983-01-01

    A cooperative advanced digital research experiment (CADRE) was established by the National Aeronautics and Space Administration (NASA) and the Royal Aircraft Establishment (RAE), in which nonlinear control algorithms developed by the RAE were tested on the F-8C digital fly-by-wire (DFBW) aircraft based at the Dryden Flight Research Facility. In the initial phase of the collaboration, some variable-gain algorithms, referred to collectively as variable integral control to optimize response (VICTOR) algorithms, were fight tested. With VICTOR, various measures available within the control system are used to vary gains and time-constants within the closed loop and thereby enhance the control capability of the system, while reducing the adverse effects of sensor noise on the control surfaces. A review of design procedures for VICTOR and results of preliminary flight tests are presented. the F-8C aircraft is operated in the remotely augmented vehicle (RAV) mode, with the control laws implemented as FORTRAN programs on a ground-based computer. Pilot commands and sensor information are telemetered to the ground, where the data are processed to form surface commands which are then telemetered to the ground, where the data are processed to form surface commands which are then telemetered back to the aircraft. The RAV mode represents a single-string (simplex) system and is therefore vulnerable to a hardover since comparison monitoring is not possible. Hence, extensive error checking is conducted on both the ground and airborne computers to prevent the development of potentially hazardous situations. Experience with the RAV monitoring and validation procedures is described.

  12. Deletion of the HCN channel auxiliary subunit TRIP8b impairs hippocampal Ih localization and function and promotes antidepressant behavior in mice

    PubMed Central

    Lewis, Alan S.; Vaidya, Sachin P.; Blaiss, Cory A.; Liu, Zhiqiang; Stoub, Travis R.; Brager, Darrin H.; Chen, Xiangdong; Bender, Roland A.; Estep, Chad M.; Popov, Andrey B.; Kang, Catherine E.; Van Veldhoven, Paul P.; Bayliss, Douglas A.; Nicholson, Daniel A.; Powell, Craig M.; Johnston, Daniel; Chetkovich, Dane M.

    2011-01-01

    Output properties of neurons are greatly shaped by voltage-gated ion channels, whose biophysical properties and localization within axodendritic compartments serve to significantly transform the original input. The hyperpolarization-activated current, Ih, is mediated by HCN channels and plays a fundamental role in influencing neuronal excitability by regulating both membrane potential and input resistance. In neurons such as cortical and hippocampal pyramidal neurons, the subcellular localization of HCN channels plays critical functional role, yet mechanisms controlling HCN channel trafficking are not fully understood. Because ion channel function and localization are often influenced by interacting proteins, we generated a knockout mouse lacking the HCN channel auxiliary subunit, TRIP8b. Eliminating expression of TRIP8b dramatically reduced Ih expression in hippocampal pyramidal neurons. Loss of Ih-dependent membrane voltage properties was attributable to reduction of HCN channels on the neuronal surface, and there was a striking disruption of the normal expression pattern of HCN channels in pyramidal neuron dendrites. In heterologous cells and neurons, absence of TRIP8b increased HCN subunit targeting to and degradation by lysosomes. Mice lacking TRIP8b demonstrated motor learning deficits and enhanced resistance to multiple tasks of behavioral despair with high predictive validity for antidepressant efficacy. We observed similar resistance to behavioral despair in distinct mutant mice lacking HCN1 or HCN2. These data demonstrate that interaction with the auxiliary subunit TRIP8b is a major mechanism underlying proper expression of HCN channels and Ih in vivo, and suggest that targeting Ih may provide a novel approach to treatment of depression. PMID:21593326

  13. Identification of fibroblast growth factor-8b target genes associated with early and late cell cycle events in breast cancer cells.

    PubMed

    Nilsson, E M; Brokken, L J S; Narvi, E; Kallio, M J; Härkönen, P L

    2012-07-06

    Fibroblast growth factor-8 (FGF-8) is implicated in the development and progression of breast cancer and its levels are frequently elevated in breast tumors. The mechanisms driving FGF-8-mediated tumorigenesis are not well understood. Herein we aimed to identify target genes associated with FGF-8b-mediated breast cancer cell proliferation by carrying out a cDNA microarray analysis of genes expressed in estrogen receptor negative S115 breast cancer cells treated with FGF-8b for various time periods in comparison with those expressed in non-treated cells. Gene and protein expression was validated for selected genes by qPCR and western blotting respectively. Furthermore, using TRANSBIG data, the expression of human orthologs of FGF-8-regulated genes was correlated to the Nottingham prognostic index and estrogen receptor status. The analysis revealed a number of significantly up- and down-regulated genes in response to FGF-8b at all treatment times. The most differentially expressed genes were genes related to cell cycle regulation, mitosis, cancer, and cell death. Several key regulators of early cell cycle progression such as Btg2 and cyclin D1, as well as regulators of mitosis, including cyclin B, Plk1, survivin, and aurora kinase A, were identified as novel targets for FGF-8b, some of which were additionally shown to correlate with prognosis and ER status in human breast cancer. The results suggest that in stimulation of proliferation FGF-8b not only promotes cell cycle progression through the G1 restriction point but also regulates key proteins involved in chromosomal segregation during mitosis and cytokinesis of breast cancer cells.

  14. Oxidation of Slurry Aluminide Coatings on Cast Stainless Steel Alloy CF8C-Plus at 800oC in Water Vapor

    SciTech Connect

    Haynes, James A; Armstrong, Beth L; Dryepondt, Sebastien N; Kumar, Deepak; Zhang, Ying

    2013-01-01

    A new, cast austenitic stainless steel, CF8C-Plus, has been developed for a wide range of high temperature applications, including diesel exhaust components, turbine casings and turbocharger housings. CF8C-Plus offers significant improvements in creep rupture life and creep rupture strength over standard CF8C steel. However, at higher temperatures and in more aggressive environments, such as those containing significant water vapor, an oxidation-resistant protective coating will be necessary. The oxidation behavior of alloys CF8C and CF8C-Plus with various aluminide coatings were compared at 800oC in air plus 10 vol% water vapor. Due to their affordability, slurry aluminides were the primary coating system of interest, although chemical vapor deposition (CVD) and pack cementation coatings were also compared. Additionally, a preliminary study of the low cycle fatigue behavior of aluminized CF8C-Plus was conducted at 800oC. Each type of coating provided substantial improvements in oxidation behavior, with simple slurry aluminides showing very good oxidation resistance after 4,000 h testing in water vapor. Preliminary low cycle fatigue results indicated that thicker aluminide coatings degraded high temperature fatigue properties of CF8C-Plus, whereas thinner coatings did not. Results suggest that appropriately designed slurry aluminide coatings are a viable option for economical, long-term oxidation protection of austenitic stainless steels in water vapor.

  15. Expression of the Multimeric and Highly Immunogenic Brucella spp. Lumazine Synthase Fused to Bovine Rotavirus VP8d as a Scaffold for Antigen Production in Tobacco Chloroplasts

    PubMed Central

    Alfano, E. Federico; Lentz, Ezequiel M.; Bellido, Demian; Dus Santos, María J.; Goldbaum, Fernando A.; Wigdorovitz, Andrés; Bravo-Almonacid, Fernando F.

    2015-01-01

    Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein which can accommodate foreign polypeptides or protein domains fused to its N-termini, markedly increasing their immunogenicity. The inner core domain (VP8d) of VP8 spike protein from bovine rotavirus is responsible for viral adhesion to sialic acid residues and infection. It also displays neutralizing epitopes, making it a good candidate for vaccination. In this work, the BLS scaffold was assessed for the first time in plants for recombinant vaccine development by N-terminally fusing BLS to VP8d and expressing the resulting fusion (BLSVP8d) in tobacco chloroplasts. Transplastomic plants were obtained and characterized by Southern, northern and western blot. BLSVP8d was highly expressed, representing 40% of total soluble protein (4.85 mg/g fresh tissue). BLSVP8d remained soluble and stable during all stages of plant development and even in lyophilized leaves stored at room temperature. Soluble protein extracts from fresh and lyophilized leaves were able to induce specific neutralizing IgY antibodies in a laying hen model. This work presents BLS as an interesting platform for highly immunogenic injectable, or even oral, subunit vaccines. Lyophilization of transplastomic leaves expressing stable antigenic fusions to BLS would further reduce costs and simplify downstream processing, purification and storage, allowing for more practical vaccines. PMID:26779198

  16. Measurement of the rate of nu(e) + d --> p + p + e(-) interactions produced by (8)B solar neutrinos at the Sudbury Neutrino Observatory.

    PubMed

    Ahmad, Q R; Allen, R C; Andersen, T C; Anglin, J D; Bühler, G; Barton, J C; Beier, E W; Bercovitch, M; Bigu, J; Biller, S; Black, R A; Blevis, I; Boardman, R J; Boger, J; Bonvin, E; Boulay, M G; Bowler, M G; Bowles, T J; Brice, S J; Browne, M C; Bullard, T V; Burritt, T H; Cameron, K; Cameron, J; Chan, Y D; Chen, M; Chen, H H; Chen, X; Chon, M C; Cleveland, B T; Clifford, E T; Cowan, J H; Cowen, D F; Cox, G A; Dai, Y; Dai, X; Dalnoki-Veress, F; Davidson, W F; Doe, P J; Doucas, G; Dragowsky, M R; Duba, C A; Duncan, F A; Dunmore, J; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Ferraris, A P; Ford, R J; Fowler, M M; Frame, K; Frank, E D; Frati, W; Germani, J V; Gil, S; Goldschmidt, A; Grant, D R; Hahn, R L; Hallin, A L; Hallman, E D; Hamer, A; Hamian, A A; Haq, R U; Hargrove, C K; Harvey, P J; Hazama, R; Heaton, R; Heeger, K M; Heintzelman, W J; Heise, J; Helmer, R L; Hepburn, J D; Heron, H; Hewett, J; Hime, A; Howe, M; Hykawy, J G; Isaac, M C; Jagam, P; Jelley, N A; Jillings, C; Jonkmans, G; Karn, J; Keener, P T; Kirch, K; Klein, J R; Knox, A B; Komar, R J; Kouzes, R; Kutter, T; Kyba, C C; Law, J; Lawson, I T; Lay, M; Lee, H W; Lesko, K T; Leslie, J R; Levine, I; Locke, W; Lowry, M M; Luoma, S; Lyon, J; Majerus, S; Mak, H B; Marino, A D; McCauley, N; McDonald, A B; McDonald, D S; McFarlane, K; McGregor, G; McLatchie, W; Meijer Drees, R; Mes, H; Mifflin, C; Miller, G G; Milton, G; Moffat, B A; Moorhead, M; Nally, C W; Neubauer, M S; Newcomer, F M; Ng, H S; Noble, A J; Norman, E B; Novikov, V M; O'Neill, M; Okada, C E; Ollerhead, R W; Omori, M; Orrell, J L; Oser, S M; Poon, A W; Radcliffe, T J; Roberge, A; Robertson, B C; Robertson, R G; Rowley, J K; Rusu, V L; Saettler, E; Schaffer, K K; Schuelke, A; Schwendener, M H; Seifert, H; Shatkay, M; Simpson, J J; Sinclair, D; Skensved, P; Smith, A R; Smith, M W; Starinsky, N; Steiger, T D; Stokstad, R G; Storey, R S; Sur, B; Tafirout, R; Tagg, N; Tanner, N W; Taplin, R K; Thorman, M; Thornewell, P; Trent, P T; Tserkovnyak, Y I; Van Berg, R; Van de Water, R G; Virtue, C J; Waltham, C E; Wang, J X; Wark, D L; West, N; Wilhelmy, J B; Wilkerson, J F; Wilson, J; Wittich, P; Wouters, J M; Yeh, M

    2001-08-13

    Solar neutrinos from (8)B decay have been detected at the Sudbury Neutrino Observatory via the charged current (CC) reaction on deuterium and the elastic scattering (ES) of electrons. The flux of nu(e)'s is measured by the CC reaction rate to be straight phi(CC)(nu(e)) = 1.75 +/- 0.07(stat)(+0.12)(-0.11)(syst) +/- 0.05(theor) x 10(6) cm(-2) s(-1). Comparison of straight phi(CC)(nu(e)) to the Super-Kamiokande Collaboration's precision value of the flux inferred from the ES reaction yields a 3.3 sigma difference, assuming the systematic uncertainties are normally distributed, providing evidence of an active non- nu(e) component in the solar flux. The total flux of active 8B neutrinos is determined to be 5.44+/-0.99 x 10(6) cm(-2) s(-1).

  17. The isolation and partial characterization of the glycolipids of BP8/C3H ascites-sarcoma cells

    PubMed Central

    Gray, G. M.

    1965-01-01

    1. The total lipid was extracted from BP8/C3H ascites-sarcoma cells with acetone, light petroleum, pyridine and chloroform–methanol successively. Each extract was treated with mild alkali. The alkali-stable lipids from the pyridine and chloroform–methanol extracts, which included the glycolipids, were fractionated on silicic acid and silica gel G columns. 2. The total yield of glycolipid was about 60 mg./100 g. dry wt. of tumour cells, about 0·4% of the total lipid. Four classes of glycolipid were isolated and characterized as ceramide monohexoside (G1), ceramide dihexoside (G2), ceramide trihexoside (G3) and ceramide hexosaminyltrihexoside (G4). 3. G1, G2, G3 and G4 constituted 55, 21, 9 and 15% of the total glycolipid respectively. 4. G1 was a mixture of ceramide glucoside (70%) and ceramide galactoside. 5. The general structures of the oligosaccharide moieties of G2, G3 and G4 were elucidated by partial acid hydrolysis of the glycolipids with water-soluble polystyrenesulphonic acid. G2 was mostly ceramidelactoside with about 10% of ceramide galactosylgalactoside. G3 and G4 were probably a ceramide digalactosylglucoside and a ceramide N-acetylgalactosaminylgalactosylgalactosylglucoside respectively. 6. The fatty acid compositions of the glycolipids were very similar; lignoceric acid and nervonic acid were the major components and all contained monohydroxy acids in proportions varying from 10 to 25% of the total acids. PMID:14342256

  18. Heat and corrosion resistant cast CF8C stainless steel with improved high temperature strength and ductility

    DOEpatents

    Maziasz, Philip J.; McGreevy, Tim; Pollard, Michael James; Siebenaler, Chad W.; Swindeman, Robert W.

    2010-08-17

    A CF8C type stainless steel alloy and articles formed therefrom containing about 18.0 weight percent to about 22.0 weight percent chromium and 11.0 weight percent to about 14.0 weight percent nickel; from about 0.05 weight percent to about 0.15 weight percent carbon; from about 2.0 weight percent to about 10.0 weight percent manganese; and from about 0.3 weight percent to about 1.5 weight percent niobium. The present alloys further include less than 0.15 weight percent sulfur which provides high temperature strength both in the matrix and at the grain boundaries without reducing ductility due to cracking along boundaries with continuous or nearly-continuous carbides. The disclosed alloys also have increased nitrogen solubility thereby enhancing strength at all temperatures because nitride precipitates or nitrogen porosity during casting are not observed. The solubility of nitrogen is dramatically enhanced by the presence of manganese, which also retains or improves the solubility of carbon thereby providing additional solid solution strengthening due to the presence of manganese and nitrogen, and combined carbon.

  19. Heat and corrosion resistant cast CF8C stainless steel with improved high temperature strength and ductility

    DOEpatents

    Maziasz, Philip J.; McGreevy, Tim; Pollard, Michael James; Siebenaler, Chad W.; Swindeman, Robert W.

    2006-12-26

    A CF8C type stainless steel alloy and articles formed therefrom containing about 18.0 weight percent to about 22.0 weight percent chromium and 11.0 weight percent to about 14.0 weight percent nickel; from about 0.05 weight percent to about 0.15 weight percent carbon; from about 2.0 weight percent to about 10.0 weight percent manganese; and from about 0.3 weight percent to about 1.5 weight percent niobium. The present alloys further include less than 0.15 weight percent sulfur which provides high temperature strength both in the matrix and at the grain boundaries without reducing ductility due to cracking along boundaries with continuous or nearly-continuous carbides. The disclosed alloys also have increased nitrogen solubility thereby enhancing strength at all temperatures because nitride precipitates or nitrogen porosity during casting are not observed. The solubility of nitrogen is dramatically enhanced by the presence of manganese, which also retains or improves the solubility of carbon thereby providing additional solid solution strengthening due to the presence of manganese and nitrogen, and combined carbon.

  20. Distinction between elastic scattering of weakly bound proton- and neutron-rich nuclei: The case of 8B and 11Be

    NASA Astrophysics Data System (ADS)

    Yang, Y. Y.; Liu, X.; Pang, D. Y.

    2016-09-01

    Experimental data show that the elastic scattering cross sections of the neutron-rich nucleus 11Be are greatly reduced by the coupling effects from the breakup channels, while those of the proton-rich nucleus 8B are not. Such difference is found to persist in results of systematic calculations of 8B elastic scattering from 208Pb at 60 and 170.3 MeV and from 64Zn at 32 and 86 MeV, and 11Be elastic scattering from 208Pb at 55 and 143 MeV and from 64Zn at 29 and 66 MeV with the continuum-discretized coupled channel (CDCC) method. The Coulomb and centrifugal barriers experienced by the valence proton in the ground state of 8B, which do not exist for the valence neutron in the ground state of 11Be, are found to be the reason for such differences in the angular distributions of elastic scattering cross sections of these two weakly bound nuclei.

  1. New fluoroborate Cd8B5O15F with two different isolated borate anions prepared by an open high-temperature solution method.

    PubMed

    Yang, Yun; Dong, Xiaoyu; Pan, Shilie

    2016-04-28

    The novel cadmium fluoroborate crystal Cd8B5O15F has been successfully obtained from the reaction of the high-temperature solution method in open air for the first time. An X-ray crystallographic study of the product reveals that Cd8B5O15F crystallizes in the space group Fd3[combining macron]m. The parameters of the cubic unit cell are a = 13.972(3) Å, and Z = 8. The title compound exhibits a complicated three-dimensional (3D) network. And the crystal structure is composed of two isolated borate anions: BO3 and B(O/F)4. For the B(O/F)4 group, it consists of the occupancy of F and O with the ratio of 3 : 1. Spectroscopic properties using the IR spectrum, X-ray photoelectron spectroscopy, and Raman spectrum were measured to illustrate this feature. The thermal behavior and UV-Vis-NIR diffuse-reflectance spectrum of Cd8B5O15F are also reported in this work.

  2. WARM SPITZER OBSERVATIONS OF THREE HOT EXOPLANETS: XO-4b, HAT-P-6b, AND HAT-P-8b

    SciTech Connect

    Todorov, Kamen O.; Deming, Drake; Knutson, Heather A.; Burrows, Adam; Sada, Pedro V.; Cowan, Nicolas B.; Agol, Eric; Desert, Jean-Michel; Charbonneau, David; Fortney, Jonathan J.; Laughlin, Gregory; Langton, Jonathan; Showman, Adam P.; Lewis, Nikole K.

    2012-02-10

    We analyze Warm Spitzer/Infrared Array Camera observations of the secondary eclipses of three planets, XO-4b, HAT-P-6b, and HAT-P-8b. We measure secondary eclipse amplitudes at 3.6 {mu}m and 4.5 {mu}m for each target. XO-4b exhibits a stronger eclipse depth at 4.5 {mu}m than at 3.6 {mu}m, which is consistent with the presence of a temperature inversion. HAT-P-8b shows a stronger eclipse amplitude at 3.6 {mu}m and is best described by models without a temperature inversion. The eclipse depths of HAT-P-6b can be fitted with models with a small or no temperature inversion. We consider our results in the context of a postulated relationship between stellar activity and temperature inversion and a relationship between irradiation level and planet dayside temperature, as discussed by Knutson et al. and Cowan and Agol, respectively. Our results are consistent with these hypotheses, but do not significantly strengthen them. To measure accurate secondary eclipse central phases, we require accurate ephemerides. We obtain primary transit observations and supplement them with publicly available observations to update the orbital ephemerides of the three planets. Based on the secondary eclipse timing, we set upper boundaries for ecos ({omega}) for HAT-P-6b, HAT-P-8b, and XO-4b and find that the values are consistent with circular orbits.

  3. Improving a recombinant Zymomonas mobilis strain 8b through continuous adaptation on dilute acid pretreated corn stover hydrolysate

    SciTech Connect

    Mohagheghi, Ali; Linger, Jeffrey G.; Yang, Shihui; Smith, Holly; Dowe, Nancy; Zhang, Min; Pienkos, Philip T.

    2015-03-31

    Complete conversion of the major sugars of biomass including both the C5 and C6 sugars is critical for biofuel production processes. Several inhibitory compounds like acetate, hydroxymethylfurfural (HMF), and furfural are produced from the biomass pretreatment process leading to ‘hydrolysate toxicity,’ a major problem for microorganisms to achieve complete sugar utilization. Therefore, development of more robust microorganisms to utilize the sugars released from biomass under toxic environment is critical. In this study, we use continuous culture methodologies to evolve and adapt the ethanologenic bacterium Zymomonas mobilis to improve its ethanol productivity using corn stover hydrolysate. The results are the following: A turbidostat was used to adapt the Z. mobilis strain 8b in the pretreated corn stover liquor. The adaptation was initiated using pure sugar (glucose and xylose) followed by feeding neutralized liquor at different dilution rates. Once the turbidostat reached 60% liquor content, the cells began washing out and the adaptation was stopped. Several ‘sub-strains’ were isolated, and one of them, SS3 (sub-strain 3), had 59% higher xylose utilization than the parent strain 8b when evaluated on 55% neutralized PCS (pretreated corn stover) liquor. Using saccharified PCS slurry generated by enzymatic hydrolysis from 25% solids loading, SS3 generated an ethanol yield of 75.5% compared to 64% for parent strain 8b. Furthermore, the total xylose utilization was 57.7% for SS3 versus 27.4% for strain 8b. To determine the underlying genotypes in these new sub-strains, we conducted genomic resequencing and identified numerous single-nucleotide mutations (SNPs) that had arisen in SS3. We further performed quantitative reverse transcription PCR (qRT-PCR) on genes potentially affected by these SNPs and identified significant down-regulation of two genes, ZMO0153 and ZMO0776, in SS3 suggesting potential genetic mechanisms behind SS3

  4. Creep and Oxidation Behavior of Modified CF8C-Plus with W, Cu, Ni, and Cr

    NASA Astrophysics Data System (ADS)

    Unocic, Kinga A.; Dryepondt, Sebastien; Yamamoto, Yukinori; Maziasz, Philip J.

    2016-04-01

    The microstructures of modified CF8C-Plus (Fe-19Cr-12Ni-0.4W-3.8Mn-0.2Mo-0.6Nb-0.5Si-0.9C) with W and Cu (CF8CPWCu) and CF8CPWCu enhanced with 21Cr + 15Ni or 22Cr + 17.5Ni were characterized in the as-cast condition and after creep testing. When imaged at lower magnifications, the as-cast microstructure was similar among all three alloys as they all contained a Nb-rich interdendritic phase and Mn-based inclusions. Transmission electron microscopy (TEM) analysis showed the presence of nanoscale Cu-rich nanoprecipitates distributed uniformly throughout the matrix of CF8CPWCu, whereas in CF8CPWCu22/17, Cu precipitates were found primarily at the grain boundaries. The presence of these nanoscale Cu-rich particles, in addition to W-rich Cr23C6, nanoscale Nb carbides, and Z-phase (Nb2Cr2N2), improved the creep strength of the CF8CPWCu steel. Modification of CF8CPWCu with Cr and Ni contents slightly decreased the creep strength but significantly improved the oxidation behavior at 1073 K (800 °C). In particular, the addition of 22Cr and 17.5Ni strongly enhanced the oxidation resistance of the stainless steel resulting in a 100 degrees or greater temperature improvement, and this composition provided the best balance between improving both mechanical properties and oxidation resistance.

  5. Creep and oxidation behavior of modified CF8C-plus with W, Cu, Ni, and Cr

    SciTech Connect

    Unocic, Kinga A.; Dryepondt, Sebastien N.; Yamamoto, Yukinori; Maziasz, Philip J.

    2016-02-01

    Here, the microstructures of modified CF8C-Plus (Fe-19Cr-12Ni-0.4W-3.8Mn-0.2Mo-0.6Nb-0.5Si-0.9C) with W and Cu (CF8CPWCu) and CF8CPWCu enhanced with 21Cr + 15Ni or 22Cr + 17.5Ni were characterized in the as-cast condition and after creep testing. When imaged at lower magnifications, the as-cast microstructure was similar among all three alloys as they all contained a Nb-rich interdendritic phase and Mn-based inclusions. Transmission electron microscopy (TEM) analysis showed the presence of nanoscale Cu-rich nanoprecipitates distributed uniformly throughout the matrix of CF8CPWCu, whereas in CF8CPWCu22/17, Cu precipitates were found primarily at the grain boundaries. The presence of these nanoscale Cu-rich particles, in addition to W-rich Cr23C6, nanoscale Nb carbides, and Z-phase (Nb2Cr2N2), improved the creep strength of the CF8CPWCu steel. Modification of CF8CPWCu with Cr and Ni contents slightly decreased the creep strength but significantly improved the oxidation behavior at 1073 K (800 °C). In particular, the addition of 22Cr and 17.5Ni strongly enhanced the oxidation resistance of the stainless steel resulting in a 100 degrees or greater temperature improvement, and this composition provided the best balance between improving both mechanical properties and oxidation resistance.

  6. Creep and oxidation behavior of modified CF8C-plus with W, Cu, Ni, and Cr

    DOE PAGES

    Unocic, Kinga A.; Dryepondt, Sebastien N.; Yamamoto, Yukinori; ...

    2016-02-01

    Here, the microstructures of modified CF8C-Plus (Fe-19Cr-12Ni-0.4W-3.8Mn-0.2Mo-0.6Nb-0.5Si-0.9C) with W and Cu (CF8CPWCu) and CF8CPWCu enhanced with 21Cr + 15Ni or 22Cr + 17.5Ni were characterized in the as-cast condition and after creep testing. When imaged at lower magnifications, the as-cast microstructure was similar among all three alloys as they all contained a Nb-rich interdendritic phase and Mn-based inclusions. Transmission electron microscopy (TEM) analysis showed the presence of nanoscale Cu-rich nanoprecipitates distributed uniformly throughout the matrix of CF8CPWCu, whereas in CF8CPWCu22/17, Cu precipitates were found primarily at the grain boundaries. The presence of these nanoscale Cu-rich particles, in addition to W-richmore » Cr23C6, nanoscale Nb carbides, and Z-phase (Nb2Cr2N2), improved the creep strength of the CF8CPWCu steel. Modification of CF8CPWCu with Cr and Ni contents slightly decreased the creep strength but significantly improved the oxidation behavior at 1073 K (800 °C). In particular, the addition of 22Cr and 17.5Ni strongly enhanced the oxidation resistance of the stainless steel resulting in a 100 degrees or greater temperature improvement, and this composition provided the best balance between improving both mechanical properties and oxidation resistance.« less

  7. Evaluation of the acute behavioral effects and abuse potential of a C8-C9 isoparaffin solvent.

    PubMed

    Balster, R L; Bowen, S E; Evans, E B; Tokarz, M E

    1997-07-04

    We hypothesized that the abuse potential of certain types of inhalants could be evaluated in animals by determining the overlap in their profile of behavioral effects with that of CNS depressant drugs and other depressant-like abused inhalants. For our first attempt in evaluating a solvent with an unknown abuse potential we tested ISOPAR-E. ISOPAR-E is a mixture of predominantly C8-C9 isoparaffinic hydrocarbons that is being used more and more frequently as a solvent in industrial and consumer products, including, but not limited to, typewriter correction fluids. Presently, nothing is known about the potential for abuse of products containing this solvent. In the present studies, we compared the volatility of ISOPAR-E and the abused solvent 1,1,1-trichloroethane (TCE) in our exposure systems. Additionally, five behavioral procedures were conducted in mice to compare the effects of the two compounds. The results demonstrate that: (1) ISOPAR-E was less volatile than TCE; (2) ISOPAR-E produced a somewhat different profile of effects than did TCE as assessed with a functional observational battery; (3) unlike TCE, ISOPAR-E did not affect performance on tests of motor coordination; (4) TCE and ISOPAR-E produced concentration-related decreases in schedule-controlled operant performance with recovery from TCE being somewhat more rapid; (5) ISOPAR-E produced cross dependence in TCE-dependent mice; and (6) both TCE and ISOPAR-E produced substantial levels of ethanol-lever responding in a drug discrimination procedure, although the ethanol-like effects of ISOPAR-E only occurred at response rate decreasing concentrations. Overall, there was a poorer separation of behavioral and lethal concentrations for ISOPAR-E than for TCE. Although a somewhat different profile of behavioral effects was obtained with ISOPAR-E and TCE, we cannot say with certainty if enough similarities exist with abused inhalants to predict that ISOPAR-E would be subject to depressant-like abuse

  8. Optimized anisotropic magnetoelectric response of Fe61.6Co16.4Si10.8B11.2/PVDF/Fe61.6Co16.4Si10.8B11.2 laminates for AC/DC magnetic field sensing

    NASA Astrophysics Data System (ADS)

    Reis, S.; Silva, M. P.; Castro, N.; Correia, V.; Gutierrez, J.; Lasheras, A.; Lanceros-Mendez, S.; Martins, P.

    2016-05-01

    The anisotropic magnetoelectric (ME) effect on a Fe61.6Co16.4Si10.8B11.2/PVDF Fe61.6Co16.4Si10.8B11.2 laminate composite has been used for the development of a magnetic field sensor able to detect both the magnitude and direction of AC and DC magnetic fields. The accuracy (99% for both AC and DC sensors), linearity (92% for the DC sensor and 99% for the AC sensor) and reproducibility (99% for both sensors) indicate the suitability of the sensor for applications. Furthermore, the sensitivity of the Fe61.6Co16.4Si10.8B11.2/PVDF/Fe61.6Co16.4Si10.8B11.2 anisotropic magnetic sensor—15 and 1400 mV Oe-1 for the DC and AC fields, respectively—are the highest reported in the literature for polymer-based ME materials. Such features, combined with its flexibility, versatility, light weight, low cost and low-temperature fabrication, lead to the suitability of the developed sensor for use in magnetic sensor applications.

  9. Some comparisons of the flyover noise characteristics of DC-9 aircraft having refanned and hardwalled JT8D engines, with special reference to measurement and analysis procedures

    NASA Technical Reports Server (NTRS)

    Hosier, R. N.

    1976-01-01

    Flyover noise measurements were made (using Federal Aviation Regulations, part 36 procedures) of two DC-9 aircraft, one equipped with refanned JT8D-109 engines and the other equipped with hardwalled JT8D-9 engines. NASA analyses show a refan centerline noise reduction of about 9.1 EPNdB and 10.0 EPNdB for takeoff with cutback and 50 deg. flap landing approach, respectively. A comparison of refan and hardwall PNLTM spectra shows that the refan noise reduction may be attributed to lower jet noise levels on takeoff and reduced high-frequency tonal content on landing approach. A general description of the test procedures and results are included along with detailed descriptions of the measurement and analysis systems.

  10. Corrosion Behaviour of Ni{sub 78}Si{sub 8}B{sub 14} Alloy in 1 M NaCl Aqueous Medium at Room Temperature

    SciTech Connect

    Mathur, Shubhra; Vyas, Rishi; Sachdev, K.; Sharma, S. K.

    2010-06-29

    Potentiodynamic polarization studies were performed on the air side and wheel side of the specimen Ni{sub 78}Si{sub 8}B{sub 14} in 1 M NaCl aqueous medium at room temperature. It was observed that the corrosion current density on the air side of the specimen was less than that of the wheel side of the specimen in 1 M NaCl aqueous medium. The amorphous nature of the air side and wheel side of the specimen Ni{sub 78}Si{sub 8}B{sub 14} was confirmed by X-ray diffraction (XRD). The observed behaviour can be related to the surface morphology due to processing of the ribbons. It is suggested that the inferior corrosion resistance of the wheel side surface is attributed to higher concentration of quenched-in defects due to higher cooling rate. Weight loss studies were also carried out in order to corroborate the polarization results. The results are discussed in the paper in the light of data reported in the literature.

  11. Measurement of the nue and Total 8B Solar Neutrino Fluxes with theSudbury Neutrino Observatory Phase I Data Set

    SciTech Connect

    Aharmim, B.; Ahmad, Q.R.; Ahmed, S.N.; Allen, R.C.; Andersen,T.C.; Anglin, J.D.; Buehler, G.; Barton, J.C.; Beier, E.W.; Bercovitch,M.; Bergevin, M.; Bigu, J.; Biller, S.D.; Black, R.A.; Blevis, I.; Boardman, R.J.; Boger, J.; Bonvin, E.; Boulay, M.G.; Bowler, M.G.; Bowles, T.J.; Brice, S.J.; Browne, M.C.; Bullard, T.V.; Burritt, T.H.; Cameron, J.; Chan, Y.D.; Chen, H.H.; Chen, M.; Chen, X.; Cleveland, B.T.; Cowan, J.H.M.; Cowen, D.F.; Cox, G.A.; Currat, C.A.; Dai, X.; Dalnoki-Veress, F.; Davidson, W.F.; Deng, H.; DiMarco, M.; Doe, P.J.; Doucas, G.; Dragowsky, M.R.; Duba, C.A.; Duncan, F.A.; Dunford, M.; Dunmore, J.A.; Earle, E.D.; Elliott, S.R.; Evans, H.C.; Ewan, G.T.; Farine, J.; Fergani, H.; Ferraris, A.P.; Fleurot, F.; Ford, R.J.; Formaggio, J.A.; Fowler, M.M.; Frame, K.; Frank, E.D.; Frati, W.; Gagnon,N.; Germani, J.V.; Gil, S.; Goldschmidt, A.; Goon, J.T.M.; Graham, K.; Grant, D.R.; Guillian, E.; Hahn, R.L.; Hallin, A.L.; Hallman, E.D.; Hamer, A.S.; Hamian, A.A.; Handler, W.B.; Haq, R.U.; Hargrove, C.K.; Harvey, P.J.; Hazama, R.; Heeger, K.M.; Heintzelman, W.J.; Heise, J.; Helmer, R.L.; Henning, R.; Hepburn, J.D.; Heron, H.; Hewett, J.; Hime,A.; Howard, C.; Howe, M.A.; Huang, M.; Hykawy, J.G.; Isaac, M.C.P.; Jagam, P.; Jamieson, B.; Jelley, N.A.; Jillings, C.; Jonkmans, G.; Kazkaz, K.; Keener, P.T.; Kirch, K.; Klein, J.R.; Knox, A.B.; Komar,R.J.; Kormos, L.L.; Kos, M.; Kouzes, R.; Krueger, A.; Kraus, C.; Krauss,C.B.; Kutter, T.; Kyba, C.C.M.; Labranche, H.; Lange, R.; Law, J.; Lawson, I.T.; Lay, M.; Lee, H.W.; Lesko, K.T.; Leslie, J.R.; Levine, I.; Loach, J.C.; Locke, W.; Luoma, S.; Lyon, J.; MacLellan, R.; Majerus, S.; Mak, H.B.; Maneira, J.; Marino, A.D.; Martin, R.; McCauley, N.; McDonald,A.B.; McDonald, D.S.; McFarlane, K.; McGee, S.; McGregor, G.; MeijerDrees, R.; Mes, H.; Mifflin, C.; Miknaitis, K.K.S.; Miller, M.L.; Milton,G.; Moffat, B.A.; Monreal, B.; Moorhead, M.; Morrissette, B.; Nally,C.W.; Neubauer, M.S.; et al.

    2007-02-01

    This article provides the complete description of resultsfrom the Phase I data set of the Sudbury Neutrino Observatory (SNO). ThePhase I data set is based on a 0.65 kt-year exposure of heavy water tothe solar 8B neutrino flux. Included here are details of the SNO physicsand detector model, evaluations of systematic uncertainties, andestimates of backgrounds. Also discussed are SNO's approach tostatistical extraction of the signals from the three neutrino reactions(charged current, neutral current, and elastic scattering) and theresults of a search for a day-night asymmetry in the ?e flux. Under theassumption that the 8B spectrum is undistorted, the measurements fromthis phase yield a solar ?e flux of ?(?e) =1.76+0.05?0.05(stat.)+0.09?0.09 (syst.) x 106 cm?2 s?1, and a non-?ecomponent ?(? mu) = 3.41+0.45?0.45(stat.)+0.48?0.45 (syst.) x 106 cm?2s?1. The sum of these components provides a total flux in excellentagreement with the predictions of Standard Solar Models. The day-nightasymmetry in the ?e flux is found to be Ae = 7.0 +- 4.9 (stat.)+1.3?1.2percent (sys.), when the asymmetry in the total flux is constrained to bezero.

  12. 75 FR 50942 - Airworthiness Directives; Pratt & Whitney JT8D-7, -7A, -7B, -9, -9A, -11, -15, -15A, -17, -17A...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... JT8D-7, -7A, -7B, -9, - 9A, -11, -15, -15A, -17, -17A, -17R, and -17AR Series Turbofan Engines AGENCY..., -1A, -1B, -7, -7A, -7B, -9, -9A, -11, -15, -15A, -17, -17A, -17R, and -17AR series turbofan engines. That AD currently requires revisions to the engine manufacturer's time limits section (TLS) to...

  13. The AV-8B Decision

    DTIC Science & Technology

    1982-06-01

    flexibility in 9 operations. In Europe, defense planners began to realize that being forced to operate from a fixed number of easily targeted airfields was...attacks against hostile targets close to friendly forces which require detailed integration of each air mission with the fire and maneuver of these...is either scheduled or on-call. Scheduled close air support is that which will be delivered on specified targets at a specified time. On-cal. close

  14. Identification of a key recombinant narrows the CADASIL gene region to 8 cM and argues against allelism of CADASIL and familial hemiplegic migraine

    SciTech Connect

    Dichgans, M.; Mayer, M.; Straube, A.

    1996-02-15

    This article reports on new information regarding the genetic mapping of the human CADASIL gene region. Previously, the gene had been mapped to human chromosome 19q12. Using the identification of a chromosomal crossover, the region has been refined to an 8-cM interval. 11 refs., 2 figs., 1 tab.

  15. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  16. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  17. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  18. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  19. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  20. Ground and flight test experience with a triple redundant digital fly by wire control system. [installed in F-8C aircraft

    NASA Technical Reports Server (NTRS)

    Jarvis, C. R.; Szalai, K. J.

    1981-01-01

    A triplex digital fly by wire flight control system was developed and installed in an F-8C aircraft to provide fail operative, full authority control. Hardware and software redundancy management techniques were designed to detect and identify failures in the system. Control functions typical of those projected for future actively controlled vehicles were implemented.

  1. Wind tunnel investigation of the aerodynamic characteristics of symmetrically deflected ailerons of the F-8C airplane. [conducted in the Langley 8-foot transonic pressure tunnel

    NASA Technical Reports Server (NTRS)

    Gera, J.

    1977-01-01

    A .042-scale model of the F-8C airplane was investigated in a transonic wind tunnel at high subsonic Mach numbers and a range of angles of attack between-3 and 20 degrees. The effect of symmetrically deflected ailerons on the longitudinal aerodynamic characteristics was measured. Some data were also obtained on the lateral control effectiveness of asymmetrically deflected horizontal tail surfaces.

  2. 26 CFR 31.3401(a)(8)(C)-1 - Remuneration for services performed in Puerto Rico by citizen of the United States.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... statement is true to the best of the employee's knowledge and belief) that such employee has at all times... the employee will be a bona fide resident of Puerto Rico. The reasonable belief contemplated by section 3401(a)(8)(C) may be based upon any evidence reasonably sufficient to induce such belief,...

  3. 26 CFR 31.3401(a)(8)(C)-1 - Remuneration for services performed in Puerto Rico by citizen of the United States.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... statement is true to the best of the employee's knowledge and belief) that such employee has at all times... the employee will be a bona fide resident of Puerto Rico. The reasonable belief contemplated by section 3401(a)(8)(C) may be based upon any evidence reasonably sufficient to induce such belief,...

  4. 26 CFR 31.3401(a)(8)(C)-1 - Remuneration for services performed in Puerto Rico by citizen of the United States.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the employee will be a bona fide resident of Puerto Rico. The reasonable belief contemplated by section 3401(a)(8)(C) may be based upon any evidence reasonably sufficient to induce such belief, even... year. (b) The employer may, in the absence of cause for a reasonable belief to the contrary,...

  5. Characterization of the specific interaction between the DNA aptamer sgc8c and protein tyrosine kinase-7 receptors at the surface of T-cells by biosensing AFM.

    PubMed

    Leitner, Michael; Poturnayova, Alexandra; Lamprecht, Constanze; Weich, Sabine; Snejdarkova, Maja; Karpisova, Ivana; Hianik, Tibor; Ebner, Andreas

    2017-04-01

    We studied the interaction of the specific DNA aptamer sgc8c immobilized at the AFM tip with its corresponding receptor, the protein tyrosine kinase-7 (PTK7) embedded in the membrane of acute lymphoblastic leukemia (ALL) cells (Jurkat T-cells). Performing single molecule force spectroscopy (SMFS) experiments, we showed that the aptamer sgc8c bound with high probability (38.3 ± 7.48%) and high specificity to PTK7, as demonstrated by receptor blocking experiments and through comparison with the binding behavior of a nonspecific aptamer. The determined kinetic off-rate (koff = 5.16 s(-1)) indicates low dissociation of the sgc8c-PTK7 complex. In addition to the pulling force experiments, simultaneous topography and recognition imaging (TREC) experiments using AFM tips functionalized with sgc8c aptamers were realized on the outer regions surface of surface-immobilized Jurkat cells for the first time. This allowed determination of the distribution of PTK7 without any labeling and at near physiological conditions. As a result, we could show a homogeneous distribution of PTK7 molecules on the outer regions of ALL cells with a surface density of 325 ± 12 PTK7 receptors (or small receptor clusters) per μm(2). Graphical Abstract The specific interaction of the DNA aptamer sgc8c and protein tyrosine kinase-7 (PTK7) on acute lymphoblastic leukemia (ALL) cells was characterized. AFM based single molecule force spectroscopy (SMFS) yielded a kinetic off-rate of 5.16 s(-1) of the complex. Simultaneous topography and recognition imaging (TREC) revealed a PTK7 density of 325 ± 12 molecules or clusters per μm(2) in the cell membrane.

  6. Antimicrobial and anti-biofilm effect of Bac8c on major bacteria associated with dental caries and Streptococcus mutans biofilms.

    PubMed

    Ding, Yonglin; Wang, Wei; Fan, Meng; Tong, Zhongchun; Kuang, Rong; Jiang, WenKai; Ni, Longxing

    2014-02-01

    Dental caries is a common oral bacterial infectious disease. Its prevention and treatment requires control of the causative pathogens within dental plaque, especially Streptococcus mutans (S. mutans). Antimicrobial peptides (AMPs), one of the promising substitutes for conventional antibiotics, have been widely tested and used for controlling bacterial infections. The present study focuses on evaluating the potential of the novel AMPs cyclic bactenecin and its derivatives against bacteria associated with dental caries. The results indicate that Bac8c displayed highest activity against the bacteria tested, whereas both cyclic and linear bactenecin had weak antimicrobial activity. The cytotoxicity assay showed that Bac8c did not cause detectable toxicity at concentrations of 32-128μg/ml for 5min or 32-64μg/ml for 60min. S. mutans and Lactobacillus fermenti treated with Bac8c showed variable effects on bacterial structure via scanning electron microscopy and transmission electron microscopy. There appeared to be a large amount of extracellular debris and obvious holes on the cell surface, as well as loss of cell wall and nucleoid condensation. The BioFlux system was employed to generate S. mutans biofilms under a controlled flow, which more closely resemble the formation process of natural biofilms. Bac8c remarkably reduced the viability of cells in biofilms formed in the BioFlux system. This phenomenon was further analyzed and verified by real-time PCR results of a significant suppression of the genes involved in S. mutans biofilm formation. Taken together, this study suggests that Bac8c has a potential clinical application in preventing and treating dental caries.

  7. Independent measurement of the total active 8B solar neutrino flux using an array of 3He proportional counters at the Sudbury Neutrino Observatory.

    PubMed

    Aharmim, B; Ahmed, S N; Amsbaugh, J F; Anthony, A E; Banar, J; Barros, N; Beier, E W; Bellerive, A; Beltran, B; Bergevin, M; Biller, S D; Boudjemline, K; Boulay, M G; Bowles, T J; Browne, M C; Bullard, T V; Burritt, T H; Cai, B; Chan, Y D; Chauhan, D; Chen, M; Cleveland, B T; Cox-Mobrand, G A; Currat, C A; Dai, X; Deng, H; Detwiler, J; DiMarco, M; Doe, P J; Doucas, G; Drouin, P-L; Duba, C A; Duncan, F A; Dunford, M; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Ford, R J; Formaggio, J A; Fowler, M M; Gagnon, N; Germani, J V; Goldschmidt, A; Goon, J T M; Graham, K; Guillian, E; Habib, S; Hahn, R L; Hallin, A L; Hallman, E D; Hamian, A A; Harper, G C; Harvey, P J; Hazama, R; Heeger, K M; Heintzelman, W J; Heise, J; Helmer, R L; Henning, R; Hime, A; Howard, C; Howe, M A; Huang, M; Jagam, P; Jamieson, B; Jelley, N A; Keeter, K J; Klein, J R; Kormos, L L; Kos, M; Krüger, A; Kraus, C; Krauss, C B; Kutter, T; Kyba, C C M; Lange, R; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Loach, J C; MacLellan, R; Majerus, S; Mak, H B; Maneira, J; Martin, R; McBryde, K; McCauley, N; McDonald, A B; McGee, S; Mifflin, C; Miller, G G; Miller, M L; Monreal, B; Monroe, J; Morissette, B; Myers, A; Nickel, B G; Noble, A J; Oblath, N S; O'Keeffe, H M; Ollerhead, R W; Gann, G D Orebi; Oser, S M; Ott, R A; Peeters, S J M; Poon, A W P; Prior, G; Reitzner, S D; Rielage, K; Robertson, B C; Robertson, R G H; Rollin, E; Schwendener, M H; Secrest, J A; Seibert, S R; Simard, O; Simpson, J J; Sinclair, L; Skensved, P; Smith, M W E; Steiger, T D; Stonehill, L C; Tesić, G; Thornewell, P M; Tolich, N; Tsui, T; Tunnell, C D; Van Wechel, T; Van Berg, R; VanDevender, B A; Virtue, C J; Walker, T J; Wall, B L; Waller, D; Tseung, H Wan Chan; Wendland, J; West, N; Wilhelmy, J B; Wilkerson, J F; Wilson, J R; Wouters, J M; Wright, A; Yeh, M; Zhang, F; Zuber, K

    2008-09-12

    The Sudbury Neutrino Observatory (SNO) used an array of 3He proportional counters to measure the rate of neutral-current interactions in heavy water and precisely determined the total active (nu_x) 8B solar neutrino flux. This technique is independent of previous methods employed by SNO. The total flux is found to be 5.54_-0.31;+0.33(stat)-0.34+0.36(syst)x10(6) cm(-2) s(-1), in agreement with previous measurements and standard solar models. A global analysis of solar and reactor neutrino results yields Deltam2=7.59_-0.21;+0.19x10(-5) eV2 and theta=34.4_-1.2;+1.3 degrees. The uncertainty on the mixing angle has been reduced from SNO's previous results.

  8. A new measurement of the 7Li(d,p)8Li cross section and consequences for 7Be(p,γ)8B

    NASA Astrophysics Data System (ADS)

    Weissman, L.; Broude, C.; Goldring, G.; Hadar, R.; Hass, M.; Schwamm, F.; Shaanan, M.

    1998-02-01

    A novel scheme for measuring the cross section of the 7Be(p,γ)8B reaction, the major source of high energy neutrinos from the sun, is presented. The scheme involves a strictly uniform particle beam and overcomes some of the recognized experimental uncertainties of previous measurements. A new measurement of σ[7Li(d,p)8Li] has been carried out using this setup, and the present value of σ[7Li(d,p)8Li] = 155(8) mb at the top of the Ed(lab.) = 776 keV resonance is compared with previous measurements. A new issue regarding both the (d,p) and (p,γ) reactions has been examined: reaction-product nuclei which are backscattered out of the target. Measurements and simulations carried out in the course of this investigation are presented and discussed in the context of possible effects on the measured cross sections of these reactions.

  9. Inclusion body hepatitis associated with an outbreak of fowl adenovirus type 2 and type 8b in broiler flocks in South Africa.

    PubMed

    Maartens, Louis H; Joubert, Hilda W; Aitchison, Henry; Venter, Estelle H

    2014-12-02

    Inclusion body hepatitis is an acute disease of chickens ascribed to viruses of the genus Aviadenovirus and referred to as fowl adenovirus (FAdV). There are 12 FAdV types (FAdV1to FAdV8a and FAdV8b to FAdV11), classified into five species based on their genotype (designated FAdVA to FAdVE). A total of 218 000 chickens, 2-29 days of age, were affected over a 1-year period, all testing positive by microscopy, virus isolation and confirmation with polymerase chain reaction (PCR). Affected birds were depressed, lost body weight,were weak and had watery droppings. Pathological changes observed during necropsy indicated consistent changes in the liver, characterised by hepatomegaly, cholestasis and hepatitis. Lesions were also discernible in the spleen, kidney and gizzard wall and were characterised by splenomegaly, pinpoint haemorrhages, nephritis with haemorrhage,visceral gout and serosal ecchymosis of the gizzard wall. Histopathological lesions were most consistently observed in the liver but could also be seen in renal and splenic tissue. Virus isolation was achieved in embryonated eggs and most embryos revealed multifocalto diffuse hepatic necrosis, with a mixed cellular infiltrate of macrophages and heterophils(necro-granulomas), even in the absence of macroscopic pathology. Virus isolation results were verified by histopathology and PCR on embryonic material and further characterised by nucleotide sequence analysis. Two infectious bursal disease virus isolates were also made from the Klerksdorp flock. Nucleotide sequence analysis of the L1 hexon loop of all the FAdV isolates indicated homology (99%) with prototype strains P7-A for FAdV-2, as well as for FAdV-8b.

  10. Epidemiological and pathological investigation of fowl aviadenovirus serotypes 8b and 11 isolated from chickens with inclusion body hepatitis in Spain (2011-2013).

    PubMed

    Oliver-Ferrando, S; Dolz, R; Calderón, C; Valle, R; Rivas, R; Pérez, M; Biarnés, M; Blanco, A; Bertran, K; Ramis, A; Busquets, N; Majó, N

    2017-04-01

    Inclusion body hepatitis caused by different fowl aviadenovirus (FAdV) serotypes has been described in several countries in recent years. In Spain, from the spring of 2011 to 2013, an increased number of outbreaks in broiler and broiler breeder flocks from different regions occurred. The objectives of the present work were to carry out the molecular characterization of FAdV strains from Spanish inclusion body hepatitis cases and to study the pathogenicity and viral dynamics of these strains in specific pathogen-free (SPF) chickens. A total of 52 inclusion body hepatitis clinical cases, including 45 from broiler farms and seven from broiler breeder farms, were analysed by conventional polymerase chain reaction and sequencing targeting the FAdV hexon gene. From these, 37 strains were classified as FAdV type 8b, while the remaining 15 were classified as FAdV types 11 (n = 10), 2 (n = 4) and 8a (n = 1). In addition, two different FAdVs belonging to the genotypes 8b and 11 were used for experimental infection. Specific pathogen-free five-day-old birds were inoculated intramuscularly with a high (10(6.5) tissue culture infective dose (TCID)50/ml) or low (10(4) TCID50/ml) dose of the above-mentioned FAdVs. No mortality was observed in any of the experimental groups, and only one bird showed evident clinical signs. However, macroscopic and microscopic hepatic lesions, as well as viral DNA, were detected in birds from all infection groups. Inclusion bodies and viral DNA were also detected in the pancreas and in the small and the large intestine in some birds. Long-lasting shedding and transmission to contact birds were confirmed in all infected groups.

  11. Nqrs Data for C6H16I2N4O8 [C6H14N4O2·2(HIO3)] (Subst. No. 0932)

    NASA Astrophysics Data System (ADS)

    Chihara, H.; Nakamura, N.

    This document is part of Subvolume A `Substances Containing Ag … C10H15' of Volume 48 `Nuclear Quadrupole Resonance Spectroscopy Data' of Landolt-Börnstein - Group III `Condensed Matter'. It contains an extract of Section `3.2 Data tables' of the Chapter `3 Nuclear quadrupole resonance data' providing the NQRS data for C6H16I2N4O8 [C6H14N4O2·2(HIO3)] (Subst. No. 0932)

  12. Nqrs Data for C6H16I2N2O8 [C6H14N2O2·2(HIO3)] (Subst. No. 0931)

    NASA Astrophysics Data System (ADS)

    Chihara, H.; Nakamura, N.

    This document is part of Subvolume A `Substances Containing Ag … C10H15' of Volume 48 `Nuclear Quadrupole Resonance Spectroscopy Data' of Landolt-Börnstein - Group III `Condensed Matter'. It contains an extract of Section `3.2 Data tables' of the Chapter `3 Nuclear quadrupole resonance data' providing the NQRS data for C6H16I2N2O8 [C6H14N2O2·2(HIO3)] (Subst. No. 0931)

  13. Metallocarbohedrenes as a class of stable neutral clusters: Formation mechanism of M{sub 8}C{sub 12} (M = Ti and V)

    SciTech Connect

    Wei, S.; Guo, B.C.; Purnell, J.; Buzza, S.; Castleman, A.W. Jr.

    1992-05-28

    Evidence of the existence of metallocarbohedrenes [M{sub 8}C{sub 12} (M = Ti and V)] as a general class of stable neutral molecular clusters is reported. Studies of the formation and growth of metal-carbon clusters M{sub m}C{sub n} reveal the general mechanisms responsible for the formation of the prominent cagelike structure, M{sub 8}C{sub 12}, using a laser-based time-of-flight mass spectrometer. Investigation of the effect of the ionizing laser fluence over at least 4 orders of magnitude and studies at 1064, 532, 355, and 266 nm establish that the prominence of the M{sub 8}C{sub 12} cluster arises due to its presence as a neutral rather than as the photofragment of clusters of large size. The unusual stability of this species for both Ti and V is consistent with the proposed cagelike structure being a pentagonal dodecahedron (point group symmetry T{sub h}). 5 refs., 4 figs.

  14. Theoretical and experimental analyses of the synergism in the dielectric strength for C3F8/C2HF5 mixtures

    NASA Astrophysics Data System (ADS)

    Larin, A. V.; Meurice, N.; Gentils, F.; Fievet, C.; Vercauteren, D. P.

    2007-04-01

    Two theoretical approaches are applied in order to describe the behavior of the experimental breakdown voltage in C3F8/C2HF5 mixtures. First, available cross section data of the electron-molecule processes are scaled to simulate the dielectric strength (DS) behavior in both C3F8 and C2HF5 gases at the level of the Boltzmann equation (BE) solution corresponding to a homogeneous electric field model. Then, the DS of C3F8/C2HF5 mixtures is evaluated and compared to the experimental breakdown voltage measured using different electrode geometries as sphere/plane type via experimental techniques, i.e., power frequency and negative or positive polarity lightning impulse. Due to the limitation of the BE approach to describe the observed experimental data, the Hunter-Christophorou [J. Appl. Phys. 57, 4377 (1985)] model regarding the positive synergism phenomenon is finally applied. Both models allow us to compare relative values of the "chemical" and "physical" DS components in C3F8/C2HF5 mixtures.

  15. Binuclear complexes of bis-chelating ligands based on [1,4]dioxocino[6,5-b:7,8-b']dipyridine moieties.

    PubMed

    Casalino, Marcello; De Felice, Vincenzo; Fraldi, Natascia; Panunzi, Achille; Ruffo, Francesco

    2009-07-06

    Ligands based on a [1,4]dioxocino[6,5-b:7,8-b']dipyridine (doxpy) core were prepared and characterized. They all present two equal chelating moieties each one including one N, O, or S donor in addition to a pyridinic nitrogen. These ligands displayed high selectivity for the formation of binuclear complexes. At least one d(8) ion (Pd(II) or Pt(II)) complex was prepared for each type of ligand. The stereochemical behavior of the ligands is discussed on the basis of NMR spectra. Stable atropoisomers were obtained in the case of N-oxides or in case chiral centers were introduced in the ethereal bridge. As for the complexes, stable enantiomers appear to be in principle attainable for all the new compounds. A test on the cooperative ability of two Pd(II) centers has been grounded on the microstructure of the styrene/CO copolymer catalytically produced by a binuclear pyridine-imino complex. In fact, comparison with the microstructure of the copolymers produced by related single-site mono- and (open-chain) binuclear catalysts reveals significant difference, thus giving indication of possible synergic metal activity.

  16. An Independent Measurement of the Total Active 8B Solar Neutrino Flux Using an Array of 3He Proportional Counters at the Sudbury Neutrino Observatory

    SciTech Connect

    SNO Colla

    2008-06-05

    The Sudbury Neutrino Observatory (SNO) used an array of {sup 3}He proportional counters to measure the rate of neutral-current interactions in heavy water and precisely determined the total active ({nu}{sub x}) {sup 8}B solar neutrino flux. This technique is independent of previous methods employed by SNO. The total flux is found to be 5.54{sub -0.31}{sup +0.33}(stat){sub -0.34}{sup +0.36}(syst) x 10{sup 6} cm{sup -2}s{sup -1}, in agreement with previous measurements and standard solar models. A global analysis of solar and reactor neutrino results yields {Delta}m{sup 2} = 7.94{sub -0.26}{sup +0.42} x 10{sup -5} eV{sup 2} and {theta} = 33.8{sub -1.3}{sup +1.4} degrees. The uncertainty on the mixing angle has been reduced from SNO's previous results.

  17. Independent Measurement of the Total Active {sup 8}B Solar Neutrino Flux Using an Array of {sup 3}He Proportional Counters at the Sudbury Neutrino Observatory

    SciTech Connect

    Aharmim, B.; Chauhan, D.; Farine, J.; Fleurot, F.; Hallman, E. D.; Krueger, A.; Schwendener, M. H.; Virtue, C. J.; Ahmed, S. N.; Cai, B.; Chen, M.; DiMarco, M.; Earle, E. D.; Evans, H. C.; Ewan, G. T.; Guillian, E.; Harvey, P. J.; Keeter, K. J.; Kormos, L. L.; Kos, M.

    2008-09-12

    The Sudbury Neutrino Observatory (SNO) used an array of {sup 3}He proportional counters to measure the rate of neutral-current interactions in heavy water and precisely determined the total active ({nu}{sub x}) {sup 8}B solar neutrino flux. This technique is independent of previous methods employed by SNO. The total flux is found to be 5.54{sub -0.31}{sup +0.33}(stat){sub -0.34}{sup +0.36}(syst)x10{sup 6} cm{sup -2} s{sup -1}, in agreement with previous measurements and standard solar models. A global analysis of solar and reactor neutrino results yields {delta}m{sup 2}=7.59{sub -0.21}{sup +0.19}x10{sup -5} eV{sup 2} and {theta}=34.4{sub -1.2}{sup +1.3} degrees. The uncertainty on the mixing angle has been reduced from SNO's previous results.

  18. Serial block-face scanning electron microscopy applied to study the trafficking of 8D3-coated gold nanoparticles at the blood-brain barrier.

    PubMed

    Cabezón, Itsaso; Augé, Elisabet; Bosch, Manel; Beckett, Alison J; Prior, Ian A; Pelegrí, Carme; Vilaplana, Jordi

    2017-03-10

    Due to the physical and physiological properties of the blood-brain barrier (BBB), the transport of neurotherapeutics from blood to brain is still a pharmaceutical challenge. We previously conducted a series of experiments to explore the potential of the anti-transferrin receptor 8D3 monoclonal antibody (mAb) to transport neurotherapeutics across the BBB. In that study, gold nanoparticles (AuNPs) were coated with the 8D3 antibody and administered intravenously to mice. Transmission electron microscopy was used and a two-dimensional (2D) image analysis was performed to detect the AuNPs in the brain capillary endothelial cells (BCECs) and brain parenchyma. In the present work, we determined that serial block-face scanning electron microscopy (SBF-SEM) is a useful tool to study the transcytosis of these AuNPs across the BBB in three dimensions and we, therefore, applied it to gain more knowledge of their transcellular trafficking. The resulting 3D reconstructions provided additional information on the endocytic vesicles containing AuNPs and the endosomal processing that occurs inside BCECs. The passage from 2D to 3D analysis reinforced the trafficking model proposed in the 2D study, and revealed that the vesicles containing AuNPs are significantly larger and more complex than described in our 2D study. We also discuss tradeoffs of using this technique for our application, and conclude that together with other volume electron microscopy imaging techniques, SBF-SEM is a powerful approach that is worth of considering for studies of drug transport across the BBB.

  19. Characterization of a versatile reference instrument for traceable fluorescence measurements using different illumination and viewing geometries specified in practical colorimetry—part 2: sphere geometry (8:d)

    NASA Astrophysics Data System (ADS)

    Zwinkels, Joanne; Neil, William; Noël, Mario; Côté, Eric

    2017-02-01

    In the second part of this two-part series on the development of a versatile reference instrument at the National Research Council of Canada (NRC), we have extended the characterization of the NRC Reference Goniospectrofluorimeter to high-accuracy fluorescence measurements in a sphere geometry (8:d) that is specified in standard test methods for many practical applications in colorimetry. This builds upon the work reported in part-one of this series which described in detail the design, characterization and validation of this new instrument for realizing a total spectral radiance factor scale in a bidirectional (45a:0) geometry. To extend the measurement capabilities to a sphere geometry, it was configured with a large diameter integrating sphere accessory. Preliminary results using a substitution-mode operating procedure showed large sphere errors that were characterized and corrected for. To improve this traceability, the sphere was modified to operate in comparison-mode and this effectively eliminated many of the sphere-related errors that typically limit the accuracy of sphere-based fluorescence measurements. The performance of the instrument configured for a sphere geometry (8:d) with this modified sphere design has been validated by means of comparison measurements of both non-fluorescent and fluorescent artifacts. The reflectance component has been validated using non-fluorescent comparison samples that have been calibrated under the same geometric conditions with traceability to the NRC Absolute Reflectometer (d:0 geometry). The fluorescent-only component has been validated using near-Lambertian fluorescent reflecting materials with traceability to the NRC Reference Spectrofluorimeter (45:0 geometry), under the assumption that this component is nearly the same for these two geometries. This work has enabled NRC to provide an uninterrupted link for improved traceability of fluorescence calibrations that specify a sphere geometry. These calibration requests

  20. Volumetric Properties of the Mixture Toluene C7H8 + C10H7Cl 1-Chloronaphthalene (VMSD1511, LB4812_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Toluene C7H8 + C10H7Cl 1-Chloronaphthalene (VMSD1511, LB4812_V)' providing data from direct measurement of low-pressure thermodynamic speed of sound at variable mole fraction and constant temperature, in the single-phase region(s).

  1. Volumetric Properties of the Mixture Toluene C7H8 + C7H8O Methyl phenyl ether (VMSD1511, LB4556_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Toluene C7H8 + C7H8O Methyl phenyl ether (VMSD1511, LB4556_V)' providing data from direct measurement of low-pressure thermodynamic speed of sound at variable mole fraction and constant temperature, in the single-phase region(s).

  2. Volumetric Properties of the Mixture Toluene C7H8 + C7H8O Methyl phenyl ether (VMSD1412, LB4562_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Toluene C7H8 + C7H8O Methyl phenyl ether (VMSD1412, LB4562_V)' providing data by calculation of isentropic compressibility from low-pressure density and thermodynamic speed of sound data at variable mole fraction and constant temperature, in the single-phase region(s).

  3. The chemical activity of metal compound nanoparticles: Importance of electronic and steric effects in M8C12 (M=Ti, V, Mo) metcars

    NASA Astrophysics Data System (ADS)

    Liu, Ping; Rodriguez, José A.; Muckerman, James T.

    2004-12-01

    Density functional theory was employed to investigate the chemical activity of metal carbide nanoparticles. The present calculations indicate that M8C12 (M=Ti, V, Mo) nanoparticles exhibit a unique behavior compared to metal [M(001)] and metal carbide surfaces [M2C(001) and MC(001)]. It is found that the nanoparticles behave very reactive in spite of the high carbon concentration in some reactions, while surprisingly inert in other cases. Our study reveals that the unexpected activity is the result of the interplay of shifts in the metal d-bands and distortions in the geometry of the metal carbide nanoparticles.

  4. Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C12H22 Bicyclohexyl (HMSD1111, LB4033_H)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C12H22 Bicyclohexyl (HMSD1111, LB4033_H)' providing data from direct low-pressure calorimetric measurement of molar excess enthalpy at variable mole fraction and constant temperature.

  5. Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C12H16 Cyclohexylbenzene (HMSD1111, LB4039_H)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C12H16 Cyclohexylbenzene (HMSD1111, LB4039_H)' providing data from direct low-pressure calorimetric measurement of molar excess enthalpy at variable mole fraction and constant temperature.

  6. Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C12H16 Cyclohexylbenzene (VMSD1211, LB3967_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C12H16 Cyclohexylbenzene (VMSD1211, LB3967_V)' providing data from direct low-pressure dilatometric measurement of molar excess volume at variable mole fraction and constant temperature.

  7. Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C12H22 Bicyclohexyl (VMSD1211, LB3961_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C12H22 Bicyclohexyl (VMSD1211, LB3961_V)' providing data from direct low-pressure dilatometric measurement of molar excess volume at variable mole fraction and constant temperature.

  8. Synthesis of aluminum-rich coatings on new high-temperature cast austenitic steel CF8C-Plus by a pack cementation process

    NASA Astrophysics Data System (ADS)

    Hall, Alex Keith

    2011-12-01

    In this research, a pack cementation process is developed for coating the newly developed cast austenitic steel CF8C-Plus. The developed coating process is capable of producing pack particle free coatings on large fatigue test specimens in a horizontal laboratory tube furnace as well as smaller oxidation and creep test samples. Several methods for the production of the pack powder free Al-rich coating are presented and evaluated for samples of both sizes. The developed coating is intended to compete with coatings of a similar quality produced with chemical vapor deposition and slurry coating methods. Additionally, because CF8C-Plus has only recently become available there is currently no available data on the effect of the fabrication of an Al-rich coating on the substrates properties. This research used advanced characterization methods to evaluate the coating surface and cross-sectional features. These methods include scanning electron microscopy, X-ray diffraction, electron probe microanalysis and energy dispersive spectroscopy analysis. This is the first time that this information has been made available to the scientific community. Also, the oxidation performance of the coating will be tested and compared to other coatings developed with CVD and slurry coating methods and the preliminary results of the effect of the coating on the alloys fatigue performance will be presented.

  9. Prevalence of human rotavirus genotypes in Wuhan, China, during 2008-2011: changing trend of predominant genotypes and emergence of strains with the P[8]b subtype of the VP4 gene.

    PubMed

    Wang, Yuan-Hong; Zhou, Xuan; Ghosh, Souvik; Zhou, Dun-Jin; Pang, Bei-Bei; Peng, Jin-Song; Hu, Quan; Kobayashi, Nobumichi

    2011-12-01

    Hospital-based surveillance of rotavirus genotypes was conducted in Wuhan, China, between March 2008 and May 2011. The detection rates of group A rotavirus were 24.6% (458/1859) and 12.1% (96/795) in children and adults, respectively, with diarrhea. Among the 554 positive specimens, the most frequent genotype was G3P[8] (57.9%), followed by G1P[8] (29.4%). Compared with previous studies in Wuhan (2000-2008), the relative frequency of G3P[8] has been decreasing year by year, while the predominant genotype G3 shifted to G1 in 2011. In the present study, a rare P[8]b subtype of the VP4 gene (OP354-like P[8]) was identified in nine strains. Full-length sequences of VP7, VP4, VP6 and NSP4 genes of two G9P[8]b strains (RVA/Human-wt/CHN/E1545/2009/G9P[8]b and RVA/Human-wt/CHN/Z1108/2008/G9P[8]b) were determined for phylogenetic analysis. The four genes of these strains were closely related to one another, and the G9-VP7 genes of these strains belonged to lineage III, which contains globally spreading G9 rotaviruses. The full-length sequence of VP4 gene segments of the P[8]b strains in Wuhan clustered with those of P[8]b strains in Vietnam, Russia and Belgium, while they were distinct from those of the OP354 strain from Malawi and Bangladeshi strains. The VP6 and NSP4 genes of two P[8]b strains belonged to the I1 and E1 genotype, respectively, and clustered with those of strains belonging to Wa-like human rotaviruses from various Asian countries. These findings indicate the changing epidemiologic trend of rotavirus genotypes in Wuhan, i.e., the shift of the predominant type from G3 to G1 and the emergence of P[8]b strains genetically related to those distributed in other Asian countries.

  10. Data integrity and electronic calibrations for the Neutral Current Detector phase measurement of the 8B solar neutrino flux at the Sudbury Neutrino Observatory

    NASA Astrophysics Data System (ADS)

    Cox-Mobrand, Gary A.

    The Sudbury Neutrino Observatory (SNO) is a heavy water Cherenkov detector that observed solar neutrinos via elastic-scattering, charge-current and neutral-current interactions. SNO was designed to measure the flux the total 8B solar neutrino flux in three separate phases, making each measurement under a different set of detector conditions and detection mechanisms. In the third phase, an array of 3He proportional counters was installed, called Neutral Current Detectors (NCDs), which detected neutrons liberated in the neutral-current interactions with deuterium. The neutrino flux can be measured in the NCD phase by identification of neutron capture events via pulse-shape analysis techniques. To accomplish this, the transformation of the neutron capture signals caused by the NCD electronics and data acquisition system (NCD DAQ) must be well known. The NCD DAQ electronics model was developed and quantified, resulting in a small contribution to the systematic uncertainties of neutron identification. Of the four currently proposed neutron identification methods, the parameters which characterize the logarithmic amplification of pulse shapes contribute 1.65%, 0.65%, 0.05% and 0.0% to the systematic uncertainty in the number of identified neutrons. A mechanical problem in two NCDs was discovered that caused the detectors to disconnect from the signal cable with little evidence of being disconnected. The work presented here identified two NCDs that suffered from this mechanical problem and estimated the amount of time that each NCD was disconnected. The remaining NCDs are shown to be unaffected by this problem and an upper limit on the amount of time disconnected was estimated. This was accomplished by an analysis of the rate of thermal noise triggers, an instrumental background noise event. The detected rates of background alphas were also measured to test for anomalously low rates. It was determined that these two NCDs should be removed from the final neutrino flux

  11. The primary structure of component 8c-1, a subunit protein of intermediate filaments in wool keratin. Relationships with proteins from other intermediate filaments.

    PubMed Central

    Dowling, L M; Crewther, W G; Inglis, A S

    1986-01-01

    Component 8c-1, one of four highly homologous component-8 subunit proteins present in the microfibrils of wool, was isolated as its S-carboxymethyl derivative and its amino acid sequence was determined. Large peptides were isolated after cleaving the protein chemically or enzymically and the sequence of each was determined with an automatic Sequenator. The peptides were ordered by sequence overlaps and, in some instances, by homology with known sequences from other component-8 subunits. The C-terminal residues were identified by three procedures. Full details of the various procedures used have been deposited as Supplementary Publication SUP 50133 (4 pp.) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1986) 233, 5. The result showed that the protein comprises 412 residues and has an Mr, including the N-terminal acetyl group, of 48,300. The sequence of residues 98-200 of component 8c-1 was found to correspond to the partial or complete sequences of four homologous type I helical segments previously isolated from helical fragments recovered from chymotryptic digests of microfibrillar proteins of wool [Crewther & Dowling (1971) Appl. Polym. Symp. 18, 1-20; Crewther, Gough, Inglis & McKern (1978) Text. Res. J. 48, 160-162; Gough, Inglis & Crewther (1978) Biochem. J. 173, 385]. Considered in relation to amino acid sequences of other intermediate-filament proteins, the sequence is in accord with the view that keratin filament proteins are of two types [Hanukoglu & Fuchs (1983) Cell (Cambridge, Mass.) 33, 915-924]. Filament proteins from non-keratinous tissues, such as desmin, vimentin, neurofilament proteins and the glial fibrillary acidic protein, which form monocomponent filaments, constitute a third type. It is suggested that as a whole the proteins from intermediate filaments be classed as filamentins, the three types at present identified forming

  12. Crystal structures of two novel borate compounds MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8}

    SciTech Connect

    Li, H.K.; Cai, G.M.; Fan, J.J.; Jin, Z.P.; Zhou, T.T.; Chen, X.L.

    2013-06-15

    Two novel borate compounds MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8}, have been synthesized via solid-state reactions, and their crystal structures have been solved and refined from powder X-ray diffraction data. The compound MgInBO{sub 4}, which was obtained at 1190 °C, belongs to the warwickite family. It crystallizes in the Pnma space group (no. 62) with a=9.5443(1) Å, b=3.2771(1) Å, c=9.5228(1) Å, and Z=4. The fundamental building units are liner –Mg(In)O{sub 6}–[In(Mg)]{sub 2}O{sub 10}–Mg(In)O{sub 6}– chains and isolated BO{sub 3} triangles. The low-temperature phase, MgIn{sub 7/8}B{sub 7/8}O{sub 29/8}, whose crystal structure is solved ab initio by the charge-flipping method with standard chemical formula MgInBO{sub 4}, was prepared at 1080 °C. It crystallizes in the P12{sub 1}/n1 space group (no. 14) with a=17.0976(1) Å, b=3.2504(1) Å, c=5.3387(1) Å, β=96.0829(3)°, and Z=4. The structure of MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} contains [In(2)/Mg(2)]{sub 2}O{sub 10} groups, –MgO{sub 6}–InO{sub 6}– infinite ribbons and isolated BO{sub 3} triangles. The experiments and the differential thermal analysis (DTA) show the decompositions of MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} happen at about 1220 °C and 1180 °C, respectively. The comparative crystal chemistry from MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} to MgInBO{sub 4} has been discussed. Infrared spectra and UV–vis diffuse reflectance spectra of MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} were measured. - Graphical abstract: The structural transformation from the monoclinic MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} (P12{sub 1}/n1) to the orthorhombic MgInBO{sub 4} (Pnma). - Highlights: • Two novel borate compounds MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} have been synthesized via solid-state reactions for the first time. • The crystal structures of MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} have been solved and refined from powder X

  13. Dissociation Cross Sections for C2F6, c-C4F8, C3F8, CHF3, and TEOS

    NASA Astrophysics Data System (ADS)

    Morgan, W. L.

    1999-10-01

    Extensive use is made of beam measurements, ab initio calculations, and electron swarm measurements in deriving total cross sections for dissociation of C_2F_6, c-C_4F_8, C_3F_8, CHF_3, and TEOS into neutral fragments. Use is made of a numerical optimization algorithm for scaling measured or computed cross sections and manipulating model cross sections in order to satisfy the measured electron transport data. These are molecules that are important in plasma processing because they readily dissociate into reactive radical fragments. Although swarm analyses cannot tell us what the specific radical fragments are, they can provide an accurate estimate of the cross section and, hence, rate coefficient for dissociation. Further details can be found on the KR&S Web site.

  14. Phase 2 program on ground test of refanned JT8D turbofan engines and nacelles for the 727 airplane. Volume 4: Airplane evaluation and analysis

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The retrofit of JT8D-109 (refan) engines are evaluated on a 727-200 airplane in terms of airworthiness, performance, and noise. Design of certifiable hardware, manufacture, and ground testing of the essential nacelle components is included along with analysis of the certifiable airplane design to ensure airworthiness compliance and to predict the in-flight performance and noise characteristics of the modified airplane. The analyses confirm that the 727 refan airplane is certifiable. The refan airplane range would be 15% less that of the baseline airplane and block fuel would be increased by 1.5% to 3%. However, with this particular 727-200 model, with a brake release gross weight of 172,500 lb (78,245 kg), it is possible to operate the airplane (with minor structural modifications) at higher gross weights and increase the range up to 15% over the 727-200 (baseline) airplane. The refan airplane FAR Part 36 noise levels would be 6 to 8 EPNdB (effective perceived noise in decibels) below the baseline. Noise footprint studies showed that approach noise contour areas are small compared to takeoff areas. The 727 refan realizes a 68% to 83% reduction in annoyance-weighted area when compared to the 727-200 over a range of gross weights and operational procedures.

  15. The crystal structure of the spinach plastocyanin double mutant G8D/L12E gives insight into its low reactivity towards photosystem 1 and cytochrome f.

    PubMed

    Jansson, Hanna; Okvist, Mats; Jacobson, Frida; Ejdebäck, Mikael; Hansson, Orjan; Sjölin, Lennart

    2003-12-08

    Plastocyanin (Pc) is a copper-containing protein, which functions as an electron carrier between the cytochrome b(6)f and photosystem 1 (PS1) complexes in the photosynthetic electron transfer (ET) chain. The ET is mediated by His87 situated in the hydrophobic surface in the north region of Pc. Also situated in this region is Leu12, which mutated to other amino acids severely disturbs the ET from cytochrome f and to PS1, indicating the importance of the hydrophobic surface. The crystal structure of the Pc double mutant G8D/L12E has been determined to 2.0 A resolution, with a crystallographic R-factor of 18.3% (R(free)=23.2%). A comparison with the wild-type structure reveals that structural differences are limited to the sites of the mutations. In particular, there is a small but significant change in the hydrophobic surface close to His87. Evidently, this leads to a mismatch in the reactive complex with the redox partners. For PS1 this results in a 20 times weaker binding and an eightfold slower ET as determined by kinetic measurements. The mutations that have been introduced do not affect the optical absorption spectrum. However, there is a small change in the EPR spectrum, which can be related to changes in the copper coordination geometry.

  16. HWMA/RCRA CLOSURE PLAN FOR THE MATERIALS TEST REACTOR WING (TRA-604) LABORATORY COMPONENTS VOLUNTARY CONSENT ORDER ACTION PLAN VCO-5.8 D REVISION2

    SciTech Connect

    KIRK WINTERHOLLER

    2008-02-25

    This Hazardous Waste Management Act/Resource Conservation and Recovery Act closure plan was developed for the laboratory components of the Test Reactor Area Catch Tank System (TRA-630) that are located in the Materials Test Reactor Wing (TRA-604) at the Reactor Technology Complex, Idaho National Laboratory Site, to meet a further milestone established under Voluntary Consent Order Action Plan VCO-5.8.d. The TRA-604 laboratory components addressed in this closure plan were deferred from the TRA-630 Catch Tank System closure plan due to ongoing laboratory operations in the areas requiring closure actions. The TRA-604 laboratory components include the TRA-604 laboratory warm wastewater drain piping, undersink drains, subheaders, and the east TRA-604 laboratory drain header. Potentially contaminated surfaces located beneath the TRA-604 laboratory warm wastewater drain piping and beneath the island sinks located in Laboratories 126 and 128 (located in TRA-661) are also addressed in this closure plan. The TRA-604 laboratory components will be closed in accordance with the interim status requirements of the Hazardous Waste Management Act/Resource Conservation and Recovery Act as implemented by the Idaho Administrative Procedures Act 58.01.05.009 and 40 Code of Federal Regulations 265, Subparts G and J. This closure plan presents the closure performance standards and the methods for achieving those standards.

  17. Expression pattern of NMDA receptors reveals antiepileptic potential of apigenin 8-C-glucoside and chlorogenic acid in pilocarpine induced epileptic mice.

    PubMed

    Aseervatham, G Smilin Bell; Suryakala, U; Doulethunisha; Sundaram, S; Bose, P Chandra; Sivasudha, T

    2016-08-01

    The present study was aimed to evaluate the effect of apigenin 8-C-glucoside (Vitexin) and chlorogenic acid on epileptic mice induced by pilocarpine and explored its possible mechanisms. Intraperitonial administration of pilocarpine (85mg/kg) induced seizure in mice was assessed by behavior observations, which is significantly (p>0.05) reduced by apigenin 8-C-glucoside (AP8CG) (10mg/kg) and chlorogenic acid (CA) (5mg/kg), similar to diazepam. Seizure was accompanied by an imbalance in the levels of Gamma-aminobutyric acid (GABA) and glutamate in the pilocarpine administered group. Moreover, convulsion along with reduced acetylcholinesterase, increased monoamine oxidase and oxidative stress was observed in epileptic mice brain. AP8CG and CA significantly restored back to normal levels even at lower doses. Further, increased lipid peroxidation and nitrite content was also significantly attenuated by AP8CG and CA. However, CA was found to be more effective when compared to AP8CG. In addition, the mRNA expression of N-methyl-d-aspartate receptor (NMDAR), mGluR1 and mGlu5 was significantly (P≤0.05) inhibited by AP8CG and CA in a lower dose. The mRNA expression of GRIK1 did not differ significantly in any of the group and showed a similar pattern of expression. Our result shows that AP8CG and CA selectively inhibit NMDAR, mGluR1 and mGlu5 expression. Modification in the provoked NMDAR calcium response coupled with neuronal death. Hence, these findings underline that the polyphenolics, AP8CG and CA have exerted antiepileptic and neuroprotective activity by suppressing glutamate receptors.

  18. Conformational energy and structure in canonical and noncanonical forms of tRNA determined by temperature analysis of the rate of s(4)U8-C13 photocrosslinking.

    PubMed

    Huggins, Wayne; Shapkina, Tatjana; Wollenzien, Paul

    2007-11-01

    Bacterial tRNAs frequently have 4-thiouridine (s(4)U) modification at position 8, which is adjacent to the C13-G22-m(7)G46 base triple in the elbow region of the tRNA tertiary structure. Irradiation with light in the UVA range induces an efficient photocrosslink between s(4)U8 and C13. The temperature dependence of the rate constants for photocrosslinking between the s(4)U8 and C13 has been used to investigate the tRNA conformational energy and structure in Escherichia coli tRNA(Val), tRNA(Phe), and tRNA(fMet) under different conditions. Corrections have been made in the measured rate constants to compensate for differences in the excited state lifetimes due to tRNA identity, buffer conditions, and temperature. The resulting rate constants are related to the rate at which the s(4)U8 and C13 come into the alignment needed for photoreaction; this depends on an activation energy, attributable to the conformational potential energy that occurs during the photoreaction, and on the extent of the structural change. Different photocrosslinking rate constants and temperature dependencies occur in the three tRNAs, and these differences are due both to modest differences in the activation energies and in the apparent s(4)U8-C13 geometries. Analysis of tRNA(Val) in buffers without Mg(2+) indicate a smaller activation energy (~13 kJ mol(-1)) and a larger apparent s(4)U8-C13 distance (~12 A) compared to values for the same parameters in buffers with Mg(2+) (~26 kJ mol(-1) and 0.36 A, respectively). These measurements are a quantitative indication of the strong constraint that Mg(2+) imposes on the tRNA flexibility and structure.

  19. The d{sup 8}, d{sup 9} and d{sup 10} supermultiplets of NiH, PdH, and PtH

    SciTech Connect

    Field, R.W.; McCarthy, M.C.; Kanamori, H.; Steimle, T.

    1993-12-31

    The concept of integer oxidation states organizes an enormous body of structural and reactivity information. Yet, for gas phase diatomic molecules, oxidation states seem never to be taken seriously by either spectroscopists or quantum chemists. For MH, MO, MF, and MM{prime} diatomics, where M is a rare earth or transition metal, oxidation states can be defined by the near-integer occupancy of the (n{minus}1)d or (n{minus}2)f valence subshell. For example, the d{sup 8}, d{sup 9}, and d{sup 10} occupancies in the (NiH, PdH, PtH) family of molecules correspond respectively to M{sup 0}, M{sup +1}, and M{sup +2} oxidation states. Spin-orbit coupling plays a central role in controlling the pattern of low-lying states, the relative intensities of nominally forbidden electronic transitions, and the predissociative interaction strength between bound and repulsive electronic states. Also, since the atomic spin-orbit coupling constant {zeta}(nd) decreases by {approximately} 10% when an electron is added to the n{minus}1d subshell, the molecular spin-orbit parameters in a supermultiplet fit model can serve as diagnostic of the fractional characters of the various n{minus}1d{sup N} occupancies (i.e. oxidation states) for each of the MH, MF, and MCu electronic states. The NiH, PdH, PtH family of molecules provides an excellent opportunity to apply supermultiplet diagnostics because (i) the energy order of the d{sup 10}, d{sup 9}s, and d{sup 8}s{sup 2} configurations in the free M atom is different for (NiH, PtH) vs PdH; (ii) the n{minus}1d orbitals are maximally contracted.

  20. DC-9/JT8D refan, Phase 1. [technical and economic feasibility of retrofitting DC-9 aircraft with refan engine to achieve desired acoustic levels

    NASA Technical Reports Server (NTRS)

    1973-01-01

    Analyses and design studies were conducted on the technical and economic feasibility of installing the JT8D-109 refan engine on the DC-9 aircraft. Design criteria included minimum change to the airframe to achieve desired acoustic levels. Several acoustic configurations were studied with two selected for detailed investigations. The minimum selected acoustic treatment configuration results in an estimated aircraft weight increase of 608 kg (1,342 lb) and the maximum selected acoustic treatment configuration results in an estimated aircraft weight increase of 809 kg (1,784 lb). The range loss for the minimum and maximum selected acoustic treatment configurations based on long range cruise at 10 668 m (35,000 ft) altitude with a typical payload of 6 804 kg (15,000 lb) amounts to 54 km (86 n. mi.) respectively. Estimated reduction in EPNL's for minimum selected treatment show 8 EPNdB at approach, 12 EPNdB for takeoff with power cutback, 15 EPNdB for takeoff without power cutback and 12 EPNdB for sideline using FAR Part 36. Little difference was estimated in EPNL between minimum and maximum treatments due to reduced performance of maximum treatment. No major technical problems were encountered in the study. The refan concept for the DC-9 appears technically feasible and economically viable at approximately $1,000,000 per airplane. An additional study of the installation of JT3D-9 refan engine on the DC-8-50/61 and DC-8-62/63 aircraft is included. Three levels of acoustic treatment were suggested for DC-8-50/61 and two levels for DC-8-62/63. Results indicate the DC-8 technically can be retrofitted with refan engines for approximately $2,500,000 per airplane.

  1. Time-gated luminescence bioimaging with new luminescent nanocolloids based on [Mo6I8(C2F5COO)6](2-) metal atom clusters.

    PubMed

    Neaime, Chrystelle; Amela-Cortes, Maria; Grasset, Fabien; Molard, Yann; Cordier, Stéphane; Dierre, Benjamin; Mortier, Michel; Takei, Toshiaki; Takahashi, Kohsei; Haneda, Hajime; Verelst, Marc; Lechevallier, Séverine

    2016-11-02

    Bioimaging and cell labeling using red or near infrared phosphors emitting in the "therapeutic window" of biological tissues have recently become some of the most active research fields in modern medical diagnostics. However, because organic and inorganic autofluorophores are omnipresent in nature, very often the background signal from fluorochromes other than targeted probes has to be eliminated. This discrimination could be available using a time-gated luminescence microscopy (TGLM) technique associated with long lifetime phosphorescent nanocomposites. Here, we report new SiO2 nanostructured particle (50 nm in diameter) embedded luminescent nanosized [Mo6I8(C2F5COO)6](2-) metal atom clusters (1 nm in diameter), successfully prepared by the microemulsion technique. This combination provides new physical insight and displays red emission in biological based solution under UV-Vis excitation with long lifetimes of around 17 and 84 μs. Moreover, the nanoparticles can be internalized by cancer cells after surface functionalization by transferrin protein and clearly imaged by TGLM under excitation at 365 nm. The nanocomposites have been mainly characterized by scanning and transmission electron microscopies (SEM and HAADF-STEM), UV-Vis and photoluminescence (PL) spectroscopies.

  2. Isostructural Pd(II) and Pt(II) pyrophosphato complexes: polymorphism and unusual bond character in d8-d8 systems.

    PubMed

    Marino, Nadia; Fazen, Christopher H; Blakemore, James D; Incarvito, Christopher D; Hazari, Nilay; Doyle, Robert P

    2011-03-21

    functional theory and atoms in molecules analyses showed that a d(8)-d(8) interaction was present in 1 and 2.

  3. Ca3Na4LiBe4B10O24F: a new beryllium borate with a unique beryl borate ∞(2)[Be8B16O40F2] layer intrabridged by [B12O24] groups.

    PubMed

    Luo, Siyang; Yao, Wenjiao; Gong, Pifu; Yao, Jiyong; Lin, Zheshuai; Chen, Chuangtian

    2014-08-18

    A novel beryllium borate, Ca3Na4LiBe4B10O24F, has been discovered. It possesses a unique ∞(2)[Be8B16O40F2] layer composed of two opposite parallel [Be4B4O12F]∞ layers bridged with [B12O24] polyborates. The linkage of [B12O24] to other structural units is first found in anhydrous borates. In the ∞(2)[Be8B16O40F2] layer, multiple tunnels are arranged along different directions resided by the alkali and alkaline-earth cations. The compound remains stable in an ambient atmosphere from room temperature to the melting point at 830 °C and melts incongruently.

  4. Emission rates of C 8-C 15 VOCs from seaweed and sand in the inter-tidal zone at Mace Head, Ireland

    NASA Astrophysics Data System (ADS)

    Sartin, J. H.; Halsall, C. J.; Hayward, S.; Hewitt, C. N.

    Emission fluxes for a range of C 8-C 15 volatile organic compounds (VOCs) were determined from the seaweed Fucus spiralis (spiral wrack) and an adjacent sand surface during low tide on the coastline of Mace Head, Ireland. These two surface types, assessed using dynamic flux chamber systems, are typical of the Mace Head inter-tidal zone. A range of n-alkanes and oxygenates were routinely identified in the measurement of chamber air. Examination of the odd/even n-alkane ratios and use of the carbon preference index (CPI) suggested a biogenic source for these compounds (CPIs >2 in for all samples). Fluxes of n-pentadecane, the most predominant n-alkane, ranged from 0.2 to 5.1 μg m -2 h -1 (0.9-24 nmol m -2 h -1), while oxygenates such as nonanal and decanal had fluxes ranging from <0.1 to 4.4 μg m -2 h -1 (<0.1-31 nmol m -2 h -1) and <0.1 to 4.6 μg m -2 h -1 (<0.1-30 nmol m -2 h -1), respectively. Seaweed emission rates for n-pentadecane were correlated with photosynthetically active radiation (PAR) ( rs=0.94) while emissions from sand showed correlation with temperature ( rs=0.85). This suggests a possible biochemical route controlling the release of n-pentadecane from spiral wrack, and temperature-driven volatilisation from sand. Volatilisation from residual seawater trapped in the sand may explain the comparable flux of both n-alkanes and oxygenates from this surface. Unlike the n-alkanes, oxygenate fluxes from sand correlate with PAR, suggesting a photodependent production from organic carbon residues present in seawater. Comparison with previous flux estimates from coastal seawater indicates that the two source types ( Fucus spiralis and bare sand) are significant but not dominant sources of these VOCs.

  5. Transiting exoplanets from the CoRoT space mission. XI. CoRoT-8b: a hot and dense sub-Saturn around a K1 dwarf

    NASA Astrophysics Data System (ADS)

    Bordé, P.; Bouchy, F.; Deleuil, M.; Cabrera, J.; Jorda, L.; Lovis, C.; Csizmadia, S.; Aigrain, S.; Almenara, J. M.; Alonso, R.; Auvergne, M.; Baglin, A.; Barge, P.; Benz, W.; Bonomo, A. S.; Bruntt, H.; Carone, L.; Carpano, S.; Deeg, H.; Dvorak, R.; Erikson, A.; Ferraz-Mello, S.; Fridlund, M.; Gandolfi, D.; Gazzano, J.-C.; Gillon, M.; Guenther, E.; Guillot, T.; Guterman, P.; Hatzes, A.; Havel, M.; Hébrard, G.; Lammer, H.; Léger, A.; Mayor, M.; Mazeh, T.; Moutou, C.; Pätzold, M.; Pepe, F.; Ollivier, M.; Queloz, D.; Rauer, H.; Rouan, D.; Samuel, B.; Santerne, A.; Schneider, J.; Tingley, B.; Udry, S.; Weingrill, J.; Wuchterl, G.

    2010-09-01

    Aims: We report the discovery of CoRoT-8b, a dense small Saturn-class exoplanet that orbits a K1 dwarf in 6.2 days, and we derive its orbital parameters, mass, and radius. Methods: We analyzed two complementary data sets: the photometric transit curve of CoRoT-8b as measured by CoRoT and the radial velocity curve of CoRoT-8 as measured by the HARPS spectrometer. Results: We find that CoRoT-8b is on a circular orbit with a semi-major axis of 0.063 ± 0.001 AU. It has a radius of 0.57 ± 0.02 RJ, a mass of 0.22 ± 0.03 MJ, and therefore a mean density of 1.6 ± 0.1 g cm-3. Conclusions: With 67% of the size of Saturn and 72% of its mass, CoRoT-8b has a density comparable to that of Neptune (1.76 g cm-3). We estimate its content in heavy elements to be 47-63 {M}_⊕, and the mass of its hydrogen-helium envelope to be 7-23 {M}_⊕. At 0.063 AU, the thermal loss of hydrogen of CoRoT-8b should be no more than 0.1% over an assumed integrated lifetime of 3 Ga. Observations made with SOPHIE spectrograph at Observatoire de Haute Provence, France (PNP.07B.MOUT), and the HARPS spectrograph at ESO La Silla Observatory (081.C-0388 and 083.C-0186). The CoRoT space mission, launched on December 27, 2006, has been developed and is operated by the CNES with the contribution of Austria, Belgium, Brasil, ESA, Germany, and Spain.Both data sets are available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/520/A66

  6. Unique Four-Electron Metal-to-Cage Charge Transfer of Th to a C82 Fullerene Cage: Complete Structural Characterization of Th@C3v(8)-C82.

    PubMed

    Wang, Yaofeng; Morales-Martínez, Roser; Zhang, Xingxing; Yang, Wei; Wang, Yaxing; Rodríguez-Fortea, Antonio; Poblet, Josep M; Feng, Lai; Wang, Shuao; Chen, Ning

    2017-03-31

    Endohedral metallofullerenes (EMFs) containing lanthanides have been intensively studied in recent years. By contrast, actinide endohedral fullerenes remain largely unexplored. Herein, for the first time, we report the single crystal structure and full characterization of an actinide endohedral fullerene, Th@C82, which exhibits remarkably different electronic and spectroscopic properties compared to those of lanthanide EMFs. Single crystal X-ray crystallography unambiguously established the molecular structure as Th@C3v(8)-C82. Combined experimental and theoretical studies reveal that Th@C3v(8)-C82 is the first example of an isolated monometallofullerene with four electrons transferred from the metal to the cage, with a surprisingly large electrochemical band gap of 1.51 eV. Moreover, Th@C3v(8)-C82 displays a strong vibrationally coupled photoluminescence signal in the visible region, an extremely rare feature for both fullerenes and thorium compounds.

  7. Association of the Variants CASP8 D302H and CASP10 V410I with Breast and Ovarian Cancer Risk in BRCA1 and BRCA2 Mutation Carriers

    PubMed Central

    Engel, Christoph; Versmold, Beatrix; Wappenschmidt, Barbara; Simard, Jacques; Easton, Douglas F.; Peock, Susan; Cook, Margaret; Oliver, Clare; Frost, Debra; Mayes, Rebecca; Evans, D. Gareth; Eeles, Rosalind; Paterson, Joan; Brewer, Carole; McGuffog, Lesley; Antoniou, Antonis C.; Stoppa-Lyonnet, Dominique; Sinilnikova, Olga M.; Barjhoux, Laure; Frenay, Marc; Michel, Cécile; Leroux, Dominique; Dreyfus, Helene; Toulas, Christine; Gladieff, Laurence; Uhrhammer, Nancy; Bignon, Yves-Jean; Meindl, Alfons; Arnold, Norbert; Varon-Mateeva, Raymonda; Niederacher, Dieter; Preisler-Adams, Sabine; Kast, Karin; Deissler, Helmut; Sutter, Christian; Gadzicki, Dorothea; Chenevix-Trench, Georgia; Spurdle, Amanda B.; Chen, Xiaoqing; Beesley, Jonathan; Olsson, Håkan; Kristoffersson, Ulf; Ehrencrona, Hans; Liljegren, Annelie; van der Luijt, Rob B.; van Os, Theo A.; van Leeuwen, Flora E.; Domchek, Susan M.; Rebbeck, Timothy R.; Nathanson, Katherine L.; Osorio, Ana; Cajal, Teresa Ramón y; Konstantopoulou, Irene; Benítez, Javier; Friedman, Eitan; Kaufman, Bella; Laitman, Yael; Mai, Phuong L.; Greene, Mark H.; Nevanlinna, Heli; Aittomäki, Kristiina; Szabo, Csilla I.; Caldes, Trinidad; Couch, Fergus J.; Andrulis, Irene L.; Godwin, Andrew K.; Hamann, Ute; Schmutzler, Rita K.

    2011-01-01

    Background The genes caspase-8 (CASP8) and caspase-10 (CASP10) functionally cooperate and play a key role in the initiation of apoptosis. Suppression of apoptosis is one of the major mechanisms underlying the origin and progression of cancer. Previous case-control studies have indicated that the polymorphisms CASP8 D302H and CASP10 V410I are associated with a reduced risk of breast cancer in the general population. Methods To evaluate whether the CASP8 D302H (CASP10 V410I) polymorphisms modify breast or ovarian cancer risk in BRCA1 and BRCA2 mutation carriers, we analyzed 7,353 (7,227) subjects of white European origin provided by 19 (18) study groups that participate in the Consortium of Investigators of Modifiers of BRCA1/2 (CIM-BA). A weighted cohort approach was used to estimate hazard ratios (HR) and 95% confidence intervals (95% CI). Results The minor allele of CASP8 D302H was significantly associated with a reduced risk of breast cancer (per-allele HR, 0.85; 95% CI, 0.76–0.97; Ptrend = 0.011) and ovarian cancer (per-allele HR, 0.69; 95% CI, 0.53–0.89; Ptrend = 0.004) for BRCA1 but not for BRCA2 mutation carriers. The CASP10 V410I polymorphism was not associated with breast or ovarian cancer risk for BRCA1 or BRCA2 mutation carriers. Conclusions CASP8 D302H decreases breast and ovarian cancer risk for BRCA1 mutation carriers but not for BRCA2 mutation carriers. Impact The combined application of these and other recently identified genetic risk modifiers could in the future allow better individual risk calculation and could aid in the individualized counseling and decision making with respect to preventive options in BRCA1 mutation carriers. PMID:20978178

  8. The oxygen content of the high-temperature superconducting compound Bi(2+x)Sr(3-y)CayCu2O(8+d) with respect to varying Ca and Bi contents

    NASA Technical Reports Server (NTRS)

    Majewski, P.; Su, H.-L.; Aldinger, F.

    1995-01-01

    The oxygen content of Bi(2+x)Sr(3-y)Cu2O(8+d) (2212 phase) has been determined as a function of its cation concentration. With increasing Ca and Bi content the oxygen content increases and T(sub c) decreases. The oxygen content of Ca rich 2212 phase increases with decreasing annealing temperatures. The study shows that the T(sub c) of the 2212 phase primarily is controlled by its cation concentration.

  9. Flight effects on noise by the JT8D engine with inverted primary/fan flow as measured in the NASA-Ames 40 by 80 foot wind tunnel

    NASA Technical Reports Server (NTRS)

    Strout, F. G.

    1978-01-01

    A JT8D-17R engine with inverted primary and fan flows was tested under static conditions as well as in the NASA Ames 40 by 80 Foot Wind Tunnel to determine static and flight noise characteristics, and flow profile of a large scale engine. Test and analysis techniques developed by a previous model and JT8D engine test program were used to determine the in-flight noise. The engine with inverted flow was tested with a conical nozzle and with a plug nozzle, 20 lobe nozzle, and an acoustic shield. Wind tunnel results show that forward velocity causes significant reduction in peak PNL suppression relative to uninverted flow. The loss of EPNL suppression is relatively modest. The in-flight peak PNL suppression of the inverter with conical nozzle was 2.5 PNdb relative to a static value of 5.5 PNdb. The corresponding EPNL suppression was 4.0 EPNdb for flight and 5.0 EPNdb for static operation. The highest in-flight EPNL suppression was 7.5 EPNdb obtained by the inverter with 20 lobe nozzle and acoustic shield. When compared with the JT8D engine with internal mixer, the inverted flow configuration provides more EPNL suppression under both static and flight conditions.

  10. Influence of ball milling on atomic structure and magnetic properties of Co{sub 40}Fe{sub 22}Ta{sub 8}B{sub 30} glassy alloy

    SciTech Connect

    Taghvaei, Amir Hossein; Stoica, Mihai; Bednarčik, Jozef; Kaban, Ivan; Shahabi, Hamed Shakur; Khoshkhoo, Mohsen Samadi; Janghorban, Kamal; Eckert, Jürgen

    2014-06-01

    The influence of ball milling on the atomic structure and magnetic properties of the Co{sub 40}Fe{sub 22}Ta{sub 8}B{sub 30} metallic glass with a high thermal stability and excellent soft magnetic properties has been investigated. After 14 h of milling, the obtained powders were found to consist mainly of an amorphous phase and a small fraction of the (Co,Fe){sub 21}Ta{sub 2}B{sub 6} nanocrystals. The changes in the reduced pair correlation functions suggest noticeable changes in the atomic structure of the amorphous upon ball milling. Furthermore, it has been shown that milling is accompanied by introduction of compressive and dilatational sites in the glassy phase and increasing the fluctuation of the atomic-level hydrostatic stress without affecting the coordination number of the nearest neighbors. Ball milling has decreased the thermal stability and significantly affected the magnetic properties through increasing the saturation magnetization, Curie temperature of the amorphous phase and coercivity. - Highlights: • Ball milling affected the atomic structure of Co{sub 40}Fe{sub 22}Ta{sub 8}B{sub 30} metallic glass. • Mechanically-induced crystallization started after 4 h milling. • Milling increased the fluctuation of the atomic-level hydrostatic stress in glass. • Ball milling influenced the thermal stability and magnetic properties.

  11. Crystal structure and Mössbauer studies of the isotypic Fe{sub 6}-cluster compounds RE{sub 15}[Fe{sub 8}C{sub 25}], RE=Dy, Ho

    SciTech Connect

    Davaasuren, Bambar; Dashjav, Enkhtsetseg; Kreiner, Guido; Borrmann, Horst; Weber, Sven-Ulf; Becker, Klaus-Dieter; Jochen Litterst, Fred; Kniep, Rüdiger

    2015-05-15

    The carboferrates RE{sub 15}[Fe{sub 8}C{sub 25}] (RE=Dy, Ho) were prepared from mixtures of the elements by arc-melting followed with subsequent annealing at 1373 K. The crystal structures were determined from single crystal X-ray diffraction data and revealed an isotypic relationship to Er{sub 15}[Fe{sub 8}C{sub 25}] (hP48, P321). The main feature of the crystal structure is given by Fe{sub 6} cluster units characterized by covalent Fe–Fe bonding interactions. {sup 57}Fe Mössbauer spectra of Dy{sub 15}[Fe{sub 8}C{sub 25}] were fitted by three subspectra with relative spectral weights of about 3:3:2 which is in general agreement with the crystal structure. Below 50 K, an onset of magnetic hyperfine fields at the three iron sites is observed which is supposed to be caused by dipolar fields arising from neighboring, slowly relaxing Dy magnetic moments. - Graphical abstract: Fe{sub 6}-cluster in the crystal structure of RE{sub 15}[Fe{sub 8}C{sub 25}], RE=Dy, Ho. - Highlights: • New carboferrates RE{sub 15}[Fe{sub 8}C{sub 25}] with RE=Dy, Ho have been synthesized. • The crystal structures were refined using single crystal X-ray data. • An orientational relationship between Fe{sub 6}-clusters and Fe in γ-Fe is outlined. • {sup 57}Fe Mössbauer spectra are in agreement with structural data from X-rays. • Magnetic hyperfine fields below 50 K are explained by dipolar fields from Dy atoms.

  12. 15 CFR 8b.12 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... physical or metal limitations of an otherwise qualified handicapped applicant or employee unless the... program or activity. (b) Reasonable accommodation may include: (1) Making the facilities used by the employees in the area where the program or activity is conducted, including common areas used by...

  13. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., request, or plan required to be approved by a Department official or by a recipient as a condition to... fair market value is not returned to the Federal Government. (f) Handicap means any condition or...) Handicapped person—(1) Handicapped person means any person who: (i) Has a physical or mental impairment...

  14. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., request, or plan required to be approved by a Department official or by a recipient as a condition to... fair market value is not returned to the Federal Government. (f) Handicap means any condition or...) Handicapped person—(1) Handicapped person means any person who: (i) Has a physical or mental impairment...

  15. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... information available to persons with hearing impairments, readers for persons with visual impairments, equipment adapted for use by persons with manual impairments, and other similar devices and...

  16. 15 CFR 8b.14 - Preemployment inquiries.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., That: (1) The recipient states clearly on any written questionnaire used for this purpose or makes clear orally, if no written questionnaire is used, that the information requested is intended for...

  17. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... more of the following body systems: Neurological; musculoskeletal; special sense organs; respiratory... postsecondary institution, or a public system of higher education; or (ii) A local educational agency (as defined in 20 U.S.C. 7801), system of vocational education, or other school system; (3)(i) An...

  18. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... more of the following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genito-urinary; hemic and lymphatic; skin... postsecondary institution, or a public system of higher education; or (ii) A local educational agency...

  19. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... more of the following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genito-urinary; hemic and lymphatic; skin... postsecondary institution, or a public system of higher education; or (ii) A local educational agency...

  20. 15 CFR 8b.18 - New construction.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... requirements of UFAS by the use of other methods are permitted where substantially equivalent or greater access...) of UFAS shall be interpreted to exempt from the requirements of UFAS only mechanical rooms and...

  1. 15 CFR 8b.18 - New construction.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... requirements of UFAS by the use of other methods are permitted where substantially equivalent or greater access...) of UFAS shall be interpreted to exempt from the requirements of UFAS only mechanical rooms and...

  2. 15 CFR 8b.18 - New construction.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... requirements of UFAS by the use of other methods are permitted where substantially equivalent or greater access...) of UFAS shall be interpreted to exempt from the requirements of UFAS only mechanical rooms and...

  3. 15 CFR 8b.18 - New construction.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... requirements of UFAS by the use of other methods are permitted where substantially equivalent or greater access...) of UFAS shall be interpreted to exempt from the requirements of UFAS only mechanical rooms and...

  4. 15 CFR 8b.18 - New construction.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... requirements of UFAS by the use of other methods are permitted where substantially equivalent or greater access...) of UFAS shall be interpreted to exempt from the requirements of UFAS only mechanical rooms and...

  5. 15 CFR 8b.22 - Academic adjustments.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... that impairs sensory, manual, or speaking skills as will best ensure that the results of the evaluation..., manual, or speaking skills (except where such skills are the factors that the test purports to measure..., or speaking skills. A recipient shall operate each program or activity to which this subpart...

  6. 15 CFR 8b.22 - Academic adjustments.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... that impairs sensory, manual, or speaking skills as will best ensure that the results of the evaluation..., manual, or speaking skills (except where such skills are the factors that the test purports to measure..., or speaking skills. A recipient shall operate each program or activity to which this subpart...

  7. 15 CFR 8b.13 - Employment criteria.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... sensory, manual, or speaking skills, the test results accurately to reflect the applicant's or employee's job skills aptitude, or whatever factor the test purports to measure, rather than reflecting the applicant's or employee's impaired sensory, manual, or speaking skills (except where those skills are...

  8. 15 CFR 8b.13 - Employment criteria.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... sensory, manual, or speaking skills, the test results accurately to reflect the applicant's or employee's job skills aptitude, or whatever factor the test purports to measure, rather than reflecting the applicant's or employee's impaired sensory, manual, or speaking skills (except where those skills are...

  9. 15 CFR 8b.13 - Employment criteria.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... sensory, manual, or speaking skills, the test results accurately to reflect the applicant's or employee's job skills aptitude, or whatever factor the test purports to measure, rather than reflecting the applicant's or employee's impaired sensory, manual, or speaking skills (except where those skills are...

  10. 15 CFR 8b.22 - Academic adjustments.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... that impairs sensory, manual, or speaking skills as will best ensure that the results of the evaluation..., manual, or speaking skills (except where such skills are the factors that the test purports to measure..., or speaking skills. A recipient shall operate each program or activity to which this subpart...

  11. 15 CFR 8b.13 - Employment criteria.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... sensory, manual, or speaking skills, the test results accurately to reflect the applicant's or employee's job skills aptitude, or whatever factor the test purports to measure, rather than reflecting the applicant's or employee's impaired sensory, manual, or speaking skills (except where those skills are...

  12. 15 CFR 8b.13 - Employment criteria.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... sensory, manual, or speaking skills, the test results accurately to reflect the applicant's or employee's job skills aptitude, or whatever factor the test purports to measure, rather than reflecting the applicant's or employee's impaired sensory, manual, or speaking skills (except where those skills are...

  13. 15 CFR 8b.22 - Academic adjustments.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... that impairs sensory, manual, or speaking skills as will best ensure that the results of the evaluation..., manual, or speaking skills (except where such skills are the factors that the test purports to measure..., or speaking skills. A recipient shall operate each program or activity to which this subpart...

  14. 15 CFR 8b.22 - Academic adjustments.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... that impairs sensory, manual, or speaking skills as will best ensure that the results of the evaluation..., manual, or speaking skills (except where such skills are the factors that the test purports to measure..., or speaking skills. A recipient shall operate each program or activity to which this subpart...

  15. 15 CFR 8b.8 - Notice.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... participants, beneficiaries, applicants and employees, including those with impaired vision or hearing, and... available to persons with impaired vision or hearing. (b) If a recipient publishes or uses...

  16. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... most integrated setting appropriate to the individual's needs. However, aid, benefits and services, to...) Discriminatory actions prohibited. (1) A recipient, in providing any aid, benefit, or service, may not, directly... service; (ii) Afford a qualified handicapped individual an opportunity to participate in or benefit...

  17. 15 CFR 8b.17 - Existing facilities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... vision or hearing, can obtain information as to the existence and location of services, activities and... accessible or the aid, benefit, or service must be made available at an alternative accessible site or sites. Accessibility requires nonpersonal aids to make the aid, benefit, or service accessible to mobility...

  18. 15 CFR 8b.5 - Assurances required.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... extended, or for another purpose involving the provision of similar services or benefits. (2) In case of... covenant running with the land to assure nondiscrimination for the period during which the real property is... involving the provision of similar services or benefits. (2) Where no transfer or property is involved...

  19. NR1H4 analysis in patients with progressive familial intrahepatic cholestasis, drug-induced cholestasis or intrahepatic cholestasis of pregnancy unrelated to ATP8B1, ABCB11 and ABCB4 mutations.

    PubMed

    Davit-Spraul, Anne; Gonzales, Emmanuel; Jacquemin, Emmanuel

    2012-12-01

    Farnesoid X receptor (FXR, NR1H4) controls bile acid homeostasis. NR1H4 variants may predispose to intrahepatic cholestasis of pregnancy (ICP). We report on NR1H4 analysis in eight patients with progressive familial intrahepatic cholestasis (PFIC) and in eight women with either ICP and/or drug-induced cholestasis (DIC) in whom no disease causing mutation in ATP8B1, ABCB11 and/or ABCB4 were found. No NR1H4 mutation was found in PFIC patients. In one woman with ICP/DIC, a NR1H4 heterozygous variant (c.-1G>T) was found. This suggests that a NR1H4 mutation is not or rarely involved in hepatocellular cholestasis of unknown cause.

  20. The spin-orbit angles of the transiting exoplanets WASP-1b, WASP-24b, WASP-38b and HAT-P-8b from Rossiter-McLaughlin observations

    NASA Astrophysics Data System (ADS)

    Simpson, E. K.; Pollacco, D.; Cameron, A. Collier; Hébrard, G.; Anderson, D. R.; Barros, S. C. C.; Boisse, I.; Bouchy, F.; Faedi, F.; Gillon, M.; Hebb, L.; Keenan, F. P.; Miller, G. R. M.; Moutou, C.; Queloz, D.; Skillen, I.; Sorensen, P.; Stempels, H. C.; Triaud, A.; Watson, C. A.; Wilson, P. A.

    2011-07-01

    We present observations of the Rossiter-McLaughlin effect for the transiting exoplanets WASP-1b, WASP-24b, WASP-38b and HAT-P-8b, and deduce the orientations of the planetary orbits with respect to the host stars' rotation axes. The planets WASP-24b, WASP-38b and HAT-P-8b appear to move in prograde orbits and be well aligned, having sky-projected spin-orbit angles consistent with zero: λ=-4?7 ± 4?0, 15°+33-43 and ?, respectively. The host stars have Teff < 6250 K and conform with the trend of cooler stars having low obliquities. WASP-38b is a massive planet on a moderately long period, eccentric orbit so may be expected to have a misaligned orbit given the high obliquities measured in similar systems. However, we find no evidence for a large spin-orbit angle. By contrast, WASP-1b joins the growing number of misaligned systems and has an almost polar orbit, λ=?. It is neither very massive, eccentric nor orbiting a hot host star, and therefore does not share the properties of many other misaligned systems. This work is based on observations collected with the SOPHIE spectrograph on the 1.93-m telescope at Observatoire de Haute-Provence (CNRS), France, by the SOPHIE Consortium; the Nordic Optical Telescope, operated on the island of La Palma jointly by Denmark, Finland, Iceland, Norway and Sweden, in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofisica de Canarias and the HARPS spectrograph mounted on the European Southern Observatory (ESO) 3.6-m telescope at the La Silla Observatory in Chile under proposal 084.C-0185.

  1. Ligand-dependent regulation of the activity of the orphan nuclear receptor, small heterodimer partner (SHP), in the repression of bile acid biosynthetic CYP7A1 and CYP8B1 genes.

    PubMed

    Miao, Ji; Choi, Sung-E; Seok, Sun Mi; Yang, Linda; Zuercher, William J; Xu, Yong; Willson, Timothy M; Xu, H Eric; Kemper, Jongsook Kim

    2011-07-01

    Small heterodimer partner (SHP) plays important roles in diverse biological processes by directly interacting with transcription factors and inhibiting their activities. SHP has been designated an orphan nuclear receptor, but whether its activity can be modulated by ligands has been a long-standing question. Recently, retinoid-related molecules, including 4-[3-(1-adamantyl)-4-hydroxyphenyl]-3-chlorocinnamic acid (3Cl-AHPC), were shown to bind to SHP and enhance apoptosis. We have examined whether 3Cl-AHPC acts as an agonist and increases SHP activity in the repression of bile acid biosynthetic CYP7A1 and CYP8B1 genes and delineated the underlying mechanisms. Contrary to this expectation, micromolar concentrations of 3Cl-AHPC increased CYP7A1 expression but indirectly via p38 kinase signaling. Nanomolar concentrations, however, repressed CYP7A1 expression and decreased bile acid levels in HepG2 cells, and little repression was observed when SHP was down-regulated by small hairpin RNA. Mechanistic studies revealed that 3Cl-AHPC bound to SHP, increased the interaction of SHP with liver receptor homologue (LRH)-1, a hepatic activator for CYP7A1 and CYP8B1 genes, and with repressive cofactors, Brahma, mammalian Sin3a, and histone deacetylase-1, and, subsequently, increased the occupancy of SHP and these cofactors at the promoters. Mutation of Leu-100, predicted to contact 3Cl-AHPC within the SHP ligand binding pocket by molecular modeling, severely impaired the increased interaction with LRH-1, and repression of LRH-1 activity mediated by 3Cl-AHPC. 3Cl-AHPC repressed SHP metabolic target genes in a gene-specific manner in human primary hepatocytes and HepG2 cells. These data suggest that SHP may act as a ligand-regulated receptor in metabolic pathways. Modulation of SHP activity by synthetic ligands may be a useful therapeutic strategy.

  2. Biological assessment: possible impacts of exploratory drilling in sections 8B and 18H, Naval Petroleum Reserve No. 2, Kern County, California on the endangered San Joaquin kit fox, blunt-nosed leopard lizard, and other sensitive species

    SciTech Connect

    O'Farrell, T.P.; Sauls, M.L.

    1982-07-01

    The U.S. Department of Energy proposes to drill exploratory wells on two sections, 8B and 18H, within Naval Petroleum Reserve No. 2 in western Kern County, California. The proposed sites are thought to provide habitat for the endangered San Joaquin kit fox and blunt-nosed leopard lizard, as well as two sensitive species: the giant kangaroo rat and San Joaquin antelope ground squirrel. The objective was to assess the possible impacts of the exploratory drilling on these species and their essential habitats. Although 23 potential San Joaquin kit fox den sites were found during surveys of a total of 512 ha (1280 acres) surrounding both well sites, no burrows were closer than 30 m from proposed disturbance, and most were over 200 m away. Two blunt-nosed leopard lizards were observed on private land within 8B, one was observed on private land in 18H, and two were seen on DOE portions of 18H. No evidence of blunt-nosed leopard lizards was gathered in the immediate vicinity of either proposed well site. Although 5 ha of habitat will be disturbed, there is no evidence to indicate any of the species has burrows on-site that will be lost during land clearing. Loss of habitat will be mitigated during the cleanup and restoration phases when disturbed areas will be revegetated. Increased traffic, human activities, noise and ground vibration levels, as well as illumination throughout the night, may disturb the fauna. However, these species have adapted to intensive human disturbances on Elk Hills without obvious negative effects. The short duration of the project should allow any displaced animals to return to the sites after drilling ceases.

  3. H and D attachment to naphthalene: spectra and thermochemistry of cold gas-phase 1-C10H9 and 1-C10H8D radicals and cations.

    PubMed

    Krechkivska, Olha; Wilcox, Callan M; Chan, Bun; Jacob, Rebecca; Liu, Yu; Nauta, Klaas; Kable, Scott H; Radom, Leo; Schmidt, Timothy W

    2015-04-02

    Excitation spectra of the 1H-naphthalene (1-C10H9) and 1D-naphthalene (1-C10H8D) radicals, and their cations, are obtained by laser spectroscopy and mass spectrometry of a skimmed free-jet expansion following an electrical discharge. The spectra are assigned on the basis of density functional theory calculations. Isotopic shifts in origin transitions, vibrational frequencies and ionization energies were found to be well reproduced by (time-dependent) density functional theory. Absolute bond dissociation energies, ionization energies and proton affinities were calculated using high-level quantum chemical methods.

  4. The oxygen content of the high-temperature superconducting compound Bi{sub 2+x}Sr{sub 3-y}CayCu{sub 2}O{sub 8+d} with respect to varying Ca and Bi contents

    SciTech Connect

    Majewski, P.; Su, H.L.; Aldinger, F.

    1994-12-31

    The oxygen content of Bi{sub 2+x}Sr{sub 3-y}Ca{sub y}Cu{sub 2}O{sub 8+d} (2212 phase) has been determined as a function of its cation concentration. With increasing Ca and Bi content the oxygen content increases and T{sub c} decreases. The oxygen content of Ca rich 2212 phase increases with decreasing annealing temperatures. The study shows that the T{sub c} of the 2212 phase primarily is controlled by its cation concentration.

  5. Role of IncP-1β Plasmids pWDL7::rfp and pNB8c in Chloroaniline Catabolism as Determined by Genomic and Functional Analyses

    PubMed Central

    Król, J. E.; Penrod, J. T.; McCaslin, H.; Rogers, L. M.; Yano, H.; Stancik, A. D.; Dejonghe, W.; Brown, C. J.; Parales, R. E.; Wuertz, S.

    2012-01-01

    Broad-host-range catabolic plasmids play an important role in bacterial degradation of man-made compounds. To gain insight into the role of these plasmids in chloroaniline degradation, we determined the first complete nucleotide sequences of an IncP-1 chloroaniline degradation plasmid, pWDL7::rfp and its close relative pNB8c, as well as the expression pattern, function, and bioaugmentation potential of the putative 3-chloroaniline (3-CA) oxidation genes. Based on phylogenetic analysis of backbone proteins, both plasmids are members of a distinct clade within the IncP-1β subgroup. The plasmids are almost identical, but whereas pWDL7::rfp carries a duplicate inverted catabolic transposon, Tn6063, containing a putative 3-CA oxidation gene cluster, dcaQTA1A2BR, pNB8c contains only a single copy of the transposon. No genes for an aromatic ring cleavage pathway were detected on either plasmid, suggesting that only the upper 3-CA degradation pathway was present. The dcaA1A2B gene products expressed from a high-copy-number vector were shown to convert 3-CA to 4-chlorocatechol in Escherichia coli. Slight differences in the dca promoter region between the plasmids and lack of induction of transcription of the pNB8c dca genes by 3-CA may explain previous findings that pNB8C does not confer 3-CA transformation. Bioaugmentation of activated sludge with pWDL7::rfp accelerated removal of 3-CA, but only in the presence of an additional carbon source. Successful bioaugmentation requires complementation of the upper pathway genes with chlorocatechol cleavage genes in indigenous bacteria. The genome sequences of these plasmids thus help explain the molecular basis of their catabolic activities. PMID:22101050

  6. A new approach to the synthesis of functional derivatives of nido-carborane: alkylation of [9-MeS-nido-7,8-C2B9H11]⁻.

    PubMed

    Zakharova, Maria V; Sivaev, Igor B; Anufriev, Sergey A; Timofeev, Sergey V; Suponitsky, Kyrill Yu; Godovikov, Ivan A; Bregadze, Vladimir I

    2014-04-07

    A series of asymmetrically substituted sulfonium derivatives of nido-carborane [9-R(Me)S-nido-7,8-C2B9H11] (R = Et, Pr, Bu, Bn, CH=CH2, CH2CH=CH2, CH2C≡CH, CH=C=CH2) were prepared by alkylation of the 9-methylthio-nido-7,8-carborane. The synthesized compounds are the first examples of diastereomers combining nido-carborane and sulfonium chiral centers.

  7. The results of low-speed wind tunnel tests to investigate the effects of the NASA refan JT8D engine nacelles on the stability and control characteristics of the Boeing 727-200

    NASA Technical Reports Server (NTRS)

    Shirkey, M. D.

    1973-01-01

    The results from two low-speed wind tunnel tests of the Boeing 727-200 airplane as configured with the NASA refan JT8D-109 turbofan engines are presented. The objective of these tests was to determine the effects of the refan installation on the low-speed stability and control characteristics of the 727 airplane. Four side nacelle locations were tested to insure that aerodynamic interactions of the nacelles and empennage would be optimized. The optimum location was judged to be the same as that of the production JT8D-9 engines; the current production engine mounts can be used for this location. Some small changes in the basic airplane characteristics are attributable to the refan nacelles. The flaps up longitudinal and lateral-directional stability are both slightly increased for low angles of attack and sideslip respectively. The longitudinal stability at stall is improved for both the flaps up and landing flap configurations. The high attitude characteristics of the basic airplane are not significantly altered by the refan nacelle installation. Directional control capability is not affected by the refan nacelles.

  8. Neuroprotection of Neuro2a cells and the cytokine suppressive and anti-inflammatory mode of action of resveratrol in activated RAW264.7 macrophages and C8-B4 microglia.

    PubMed

    Steiner, Nicole; Balez, Rachelle; Karunaweera, Niloo; Lind, Joanne M; Münch, Gerald; Ooi, Lezanne

    2016-05-01

    Chronic inflammation is a hallmark of neurodegenerative disease and cytotoxic levels of nitric oxide (NO) and pro-inflammatory cytokines can initiate neuronal death pathways. A range of cellular assays were used to assess the anti-inflammatory and neuroprotective action of resveratrol using murine microglial (C8-B4), macrophage (RAW264.7) and neuronal-like (Neuro2a) cell lines. We examined the release of NO by Griess assay and used a Bioplex array to measure a panel of pro- and anti-inflammatory cytokines and chemokines, in response to the inflammatory stimuli lipopolysaccharide (LPS) and interferon-γ (IFN-γ). Resveratrol was a potent inhibitor of NO and cytokine release in activated macrophages and microglia. The activity of resveratrol increased marginally in potency with longer pre-incubation times in cell culture that was not due to cytotoxicity. Using an NO donor we show that resveratrol can protect Neuro2a cells from cytotoxic concentrations of NO. The protective effect of resveratrol from pro-inflammatory signalling in RAW264.7 cells was confirmed in co-culture experiments leading to increased survival of Neuro2a cells. Together our data are indicative of the potential neuroprotective effect of resveratrol during nitrosative stress and neuroinflammation.

  9. Spin-orbit inclinations of the exoplanetary systems HAT-P-8b, HAT-P-9b, HAT-P-16b, and HAT-P-23b

    NASA Astrophysics Data System (ADS)

    Moutou, C.; Díaz, R. F.; Udry, S.; Hébrard, G.; Bouchy, F.; Santerne, A.; Ehrenreich, D.; Arnold, L.; Boisse, I.; Bonfils, X.; Delfosse, X.; Eggenberger, A.; Forveille, T.; Lagrange, A.-M.; Lovis, C.; Martinez, P.; Pepe, F.; Perrier, C.; Queloz, D.; Santos, N. C.; Ségransan, D.; Toublanc, D.; Troncin, J. P.; Vanhuysse, M.; Vidal-Madjar, A.

    2011-09-01

    We report the measurement of the spin-orbit angle of the extra-solar planets HAT-P-8 b, HAT-P-9 b, HAT-P-16 b, and HAT-P-23 b, based on spectroscopic observations performed at the Observatoire de Haute-Provence with the SOPHIE spectrograph on the 1.93-m telescope. Radial velocity measurements of the Rossiter-McLaughlin effect show the detection of an apparent prograde, aligned orbit for all systems. The projected spin-orbit angles are found to be λ = -17°+9.2-11.5, -16° ± 8°, -10° ± 16°, and +15° ± 22° for HAT-P-8, HAT-P-9, HAT-P-16, and HAT-P-23, respectively, with corresponding projected rotational velocities of 14.5 ± 0.8, 12.5 ± 1.8, 3.9 ± 0.8, and 7.8 ± 1.6 km s-1. These new results increase to 37 the number of accurately measured spin-orbit angles in transiting extrasolar systems. We conclude by drawing a tentative picture of the global behaviour of orbital alignement, involving the complexity and diversity of possible mechanisms. Based on observations collected with the SOPHIE spectrograph on the 1.93-m telescope at Observatoire de Haute-Provence (CNRS), France, by the SOPHIE Consortium (program 10A.PNP.CONS).

  10. Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C10H18 Bicyclo[4.4.0]decane (HMSD1111, LB4030_H)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C10H18 Bicyclo[4.4.0]decane (HMSD1111, LB4030_H)' providing data from direct low-pressure calorimetric measurement of molar excess enthalpy at variable mole fraction and constant temperature.

  11. Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C10H12 1,2,3,4-Tetrahydronaphthalene (VMSD1211, LB3964_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C10H12 1,2,3,4-Tetrahydronaphthalene (VMSD1211, LB3964_V)' providing data from direct low-pressure dilatometric measurement of molar excess volume at variable mole fraction and constant temperature.

  12. Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C10H18 Bicyclo[4.4.0]decane (VMSD1211, LB3958_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Cyclohexa-1,3-diene C6H8 + C10H18 Bicyclo[4.4.0]decane (VMSD1211, LB3958_V)' providing data from direct low-pressure dilatometric measurement of molar excess volume at variable mole fraction and constant temperature.

  13. Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C10H12 1,2,3,4-Tetrahydronaphthalene (HMSD1111, LB4036_H)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume B 'Binary Liquid Systems of Nonelectrolytes II' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Heat of Mixing and Solution of Cyclohexa-1,3-diene C6H8 + C10H12 1,2,3,4-Tetrahydronaphthalene (HMSD1111, LB4036_H)' providing data from direct low-pressure calorimetric measurement of molar excess enthalpy at variable mole fraction and constant temperature.

  14. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genitourinary; hemic and lymphatic; skin; and...

  15. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    .... Auxiliary aids means services or devices that enable persons with impaired sensory, manual, or speaking skills to have an equal opportunity to participate in, and enjoy the benefits of, programs or activities..., speaking, breathing, learning, and working. (3) “Has a record of such an impairment” means has a history...

  16. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    .... Auxiliary aids means services or devices that enable persons with impaired sensory, manual, or speaking skills to have an equal opportunity to participate in, and enjoy the benefits of, programs or activities..., speaking, breathing, learning, and working. (3) “Has a record of such an impairment” means has a history...

  17. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genitourinary; hemic and lymphatic; skin; and...

  18. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genitourinary; hemic and lymphatic; skin; and...

  19. A phenylacetaldehyde-flavonoid adduct, 8-C-(E-phenylethenyl)-norartocarpetin, exhibits intrinsic apoptosis and MAPK pathways-related anticancer potential on HepG2, SMMC-7721 and QGY-7703.

    PubMed

    Zheng, Zong-Ping; Yan, Yan; Xia, Ji; Zhang, Shuang; Wang, Mingfu; Chen, Jie; Xu, Yang

    2016-04-15

    Norartocarpetin, quercetin and naringenin were found to effectively inhibit 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) formation through trapping its phenylacetaldehyde and form their adducts in roast beef patties. Six adducts [8-C- or 6-C-(E-phenylethenyl) flavonoids] formed between phenylacetaldehyde and three flavonoids were detected in roast beef patties by UPLC-MS analyses and compared with their synthetic references. These flavonoid-phenylacetaldehyde adducts were synthesised and further subjected to cytotoxicity tests on three liver cancer cell lines HepG2, SMMC-7721 and QGY-7703. The adduct 8-C-(E-phenylethenyl)norartocarpetin (NARA1) was found to significantly induce cancer cell death with IC50 values about 7 μM. After pre-treating with MAPK and caspase inhibitors, alteration of the cell morphology and cleaved-PARP were detected in liver cancer cell lines administered with NARA1. These data indicated that norartocarpetin could inhibit PhIP formation in roast beef patties and form norartocarpetin-phenylacetaldehyde adducts. The adduct NARA1 has anticancer potential via intrinsic caspase-dependent and cell context-dependent MAPKs pathways.

  20. Evaluation of Cavity Collapse and Surface Crater Formation at the Norbo Underground Nuclear Test in U8c, Nevada Nuclear Security Site, and the Impact on Stability of the Ground Surface

    SciTech Connect

    Pawloski, G A

    2012-06-18

    Lawrence Livermore National Laboratory (LLNL) Containment Program performed a review of nuclear test-related data for the Norbo underground nuclear test in U8c to assist in evaluating this legacy site as a test bed for application technologies for use in On-Site Inspections (OSI) under the Comprehensive Nuclear Test Ban Treaty. This request is similar to one made for the Salut site in U8c (Pawloski, 2012b). Review of the Norbo site is complicated because the test first exhibited subsurface collapse, which was not unusual, but it then collapsed to the surface over one year later, which was unusual. Of particular interest is the stability of the ground surface above the Norbo detonation point. Proposed methods for on-site verification include radiological signatures, artifacts from nuclear testing activities, and imaging to identify alteration to the subsurface hydrogeology due to the nuclear detonation. Aviva Sussman from the Los Alamos National Laboratory (LANL) has also proposed work at this site. Both proposals require physical access at or near the ground surface of specific underground nuclear test locations at the Nevada Nuclear Security Site (NNSS), formerly the Nevada Test Site (NTS), and focus on possible activities such as visual observation, multispectral measurements, and shallow and deep geophysical surveys.

  1. MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.

    PubMed

    Wang, Youning; Li, Kexue; Chen, Liang; Zou, Yanmin; Liu, Haipei; Tian, Yinping; Li, Dongxiao; Wang, Rui; Zhao, Fang; Ferguson, Brett J; Gresshoff, Peter M; Li, Xia

    2015-07-01

    Legume root nodules convert atmospheric nitrogen gas into ammonium through symbiosis with a prokaryotic microsymbiont broadly called rhizobia. Auxin signaling is required for determinant nodule development; however, the molecular mechanism of auxin-mediated nodule formation remains largely unknown. Here, we show in soybean (Glycine max) that the microRNA miR167 acts as a positive regulator of lateral root organs, namely nodules and lateral roots. miR167c expression was up-regulated in the vasculature, pericycle, and cortex of soybean roots following inoculation with Bradyrhizobium japonicum strain USDA110 (the microsymbiont). It was found to positively regulate nodule numbers directly by repressing the target genes GmARF8a and GmARF8b (homologous genes of Arabidopsis [Arabidopsis thaliana] AtARF8 that encode auxin response factors). Moreover, the expression of miR167 and its targets was up- and down-regulated by auxin, respectively. The miR167-GmARF8 module also positively regulated nodulation efficiency under low microsymbiont density, a condition often associated with environmental stress. The regulatory role of miR167 on nodule initiation was dependent on the Nod factor receptor GmNFR1α, and it acts upstream of the nodulation-associated genes nodule inception, nodulation signaling pathway1, early nodulin40-1, NF-YA1 (previously known as HAEM activator protein2-1), and NF-YA2. miR167 also promoted lateral root numbers. Collectively, our findings establish a key role for the miR167-GmARF8 module in auxin-mediated nodule and lateral root formation in soybean.

  2. Unveiling the unfolding pathway of F5F8D disorder-associated D81H/V100D mutant of MCFD2 via multiple molecular dynamics simulations.

    PubMed

    Hamza, Adel; Wei, Ning-Ning; Johnson-Scalise, Trudy; Naftolin, Frederick; Cho, Hoon; Zhan, Chang-Guo

    2012-01-01

    Combined factor deficiency (F5F8D) is a rare autosomal recessive disorder caused by mutations in the LMAN1 or MCFD2 genes. It has been proposed that this pathogenic process occurs via a multi-step pathway involving metal loss, EF-hand-Ca21 dissociation and assembly of misfolded MCFD2-LMAN1 complex. Here, we have investigated the solution conformations of the MCFD2((D81H,V100D)) protein mutant through extensive molecular dynamics (MD) simulations. The V100D, one of the many MCFD2 mutations known to be associated to F5F8D, is difficult to be reconciled with the pathway model because it is located far from the metal sites and the MCFD2/LMAN1 interface. Consequently, an inspection of all the steps involved in D81H/V100D MCFD2 misfolding is expected to provide hints in the understanding of the molecular basis of the disease. A comparison with parallel studies carried out for the Wild-Type (WT) MCFD2 pointed out that the mutation decreases the affinity of the protein for the Ca21 ion. Multiple explicit solvents MD simulations (50 ns) performed on the two proteins revealed that in the WT protein, stable H-bond network and compact hydrophobic core region are created thus confirming a pivotal role of this region in driving the biophysical properties of the entire protein. In fact it is shown that the V100D mutation, although located far away the EF-hand domain, may induce subtle modification in the structural core of MCFD2 leading to the loosening of metal binding and to the formation of metastable intermediate states along the unfolding pathway. The native-like hydrophobic cluster formed near the V100 residue in the wild-type protein is disrupted by the negatively charged Asparagine residue. Furthermore, the presence of the D81H mutation in the EF-1 hand domain may also increase the protein unfolding rate and consequently prevent the formation of the MCFD2-LMAN1 complex. The detailed structural insights obtained from our large-scale simulations complement the clinical

  3. Infrared band intensities and global warming potentials of CF4, C2F6, C3F8, C4F10, C5F12, and C6F14

    NASA Astrophysics Data System (ADS)

    Roehl, C. M.; Boglu, D.; Brühl, C.; Moortgat, G. K.

    1995-04-01

    IR band intensities have been measured for the species: CF4, C2F6, C3F8, C4F10, C5F12, and C6F14 via Fourier transform spectroscopy and compared to previous literature values if available. Relative radiative forcing calculations have been performed using these data in order to determine the global warming potential of the particular species. The relative forcing (compared to CFC11, per volume) increases with molecular weight in the above series from 0.47 to 2.1, the GWP for a time horizon of 100 yrs from 1.1 to 4.7. This corresponds to a GWP on CO2 basis per mass of about 5000.

  4. Computational Study of the Radical Mediated Mechanism of the Formation of C8, C5, and C4 Guanine:Lysine Adducts in the Presence of the Benzophenone Photosensitizer.

    PubMed

    Thapa, Bishnu; Munk, Barbara H; Burrows, Cynthia J; Schlegel, H Bernhard

    2016-09-19

    The oxidation of guanine by triplet benzophenone in the presence of lysine has been shown to produce mono- and dilysine-substituted spiroiminodihydantion products, 8-Lys-Sp and 5,8-diLys-Sp. The potential energy surfaces for C8, C5, and C4 nucleophilic addition have been mapped out using the B3LYP/aug-cc-pVTZ//B3LYP/6-31+G(d,p) level of density functional theory with the SMD solvation model and employing methylamine as a model for the side chain of lysine. Enthalpies, barrier heights, pKa's, and reduction potentials were calculated for intermediates to find the lowest energy paths. For neutral methylamine plus guanine radical and neutral methylamine radical plus guanine, the barrier for addition at C8 is ca. 10 kcal/mol lower than that for addition at C5 and C4. The barriers for water addition at C8, C5, and C4 of guanine radical are 13-20 kcal/mol higher than that for methylamine addition at C8. Further oxidation and loss of a proton leads to 8-methylaminoguanine, the methylamino analogue of 8-oxo-7,8-dihydroguanine (8-oxoG). The barrier for the addition of a second methylamine at C5 of 8-methylaminoguanine is 4.5 kcal/mol lower than that for the corresponding addition of water. Nevertheless, if the concentration of methylamine (or lysine) is very low, water addition could be competitive with methylamine addition. This would lead to comparable fractions of 8-monosubstituted-Sp and 5-8-disubstituted-Sp, in agreement with the experimental observations.

  5. Ground state properties of trigonal prismatic and trigonal bipyramidal d1,9, d2,8, d3,7, and d5 systems: Effects of trigonal distortion

    NASA Astrophysics Data System (ADS)

    Hempel, Judith C.; Miller, Michael E.

    1981-09-01

    Empirical ligand field theory has been used to calculate ground state energy space diagrams for d1,9, d2,8, d3,7, and d5 systems of D3h symmetry and allowed ground states for trigonal bipyramidal (five coordinate) and trigonal prismatic (six coordinate) D3h geometries have been identified. We demonstrate that entirely similar behavior should be expected for trigonal prismatic and trigonal bipyramidal d5 systems. Spin-orbit calculations complete within the d5 basis are used to establish that the ESR zero field parameter D for a 6A'1(D3h) ground state will normally be negative. Normalized spherical harmonic (NSH) ligand field Hamiltonians symmetry adapted to chains based on the D6h covering group are defined for all trigonal symmetries. Multiple symmetry parameter sets, which arise for trigonal symmetries other than D3h, describe the range of orbital mixing possible. Spin-orbit and Zeeman interactions reveal the effects of orbital mixing and restrict to two the number of symmetry parameter sets possible for a trigonal system. We specifically consider the magnetic properties of the five coordinate complexes of C3v symmetry [M(Me6tren)Br]Br, with M = Cu(II) and Ni(II) using calculations for C3v symmetry which incorporate spin-orbit and Zeeman interactions and are complete within the dn basis.

  6. The results of a low speed wind tunnel test to investigate the effects of installing refan JT8D engines on the McDonnell Douglas DC-9-30

    NASA Technical Reports Server (NTRS)

    Chrisenberry, H. E.; Doss, P. G.; Kressly, A. E.; Prichard, R. D.; Thorndike, C. S.

    1973-01-01

    A low speed wind tunnel test was conducted to assess the effects of the larger JT8D refan nacelles on the stability and control characteristics of the DC-9-30, with emphasis on the deep stall regime. Deep stall pitching moment and elevator hinge moment data, and low angle of attack tail-on and tail-off pitching moment data are presented. The refan nacelle was tested in conjunction with various pylons of reduced span relative to the production DC-9-30 pylon. Also, a horizontal tail that was larger than the production tail was tested. The data show that the refan installation has a small detrimental effect on the DC-9-30 deep stall recovery capability, that recovery characteristics are essentially independent of pylon span, and that the larger horizontal tail significantly increases recovery margins. The deep stall characteristics with the refan installation, within the range of pylon spans tested, are acceptable with no additional design changes anticipated.

  7. Kasatkinite, Ba2Ca8B5Si8O32(OH)3 · 6H2O6, a new mineral from the Bazhenovskoe deposit, the Central Urals, Russia

    NASA Astrophysics Data System (ADS)

    Pekov, I. V.; Chukanov, N. V.; Filinchuk, Ya. E.; Zadov, A. E.; Kononkova, N. N.; Epanchintsev, S. G.; Kaden, P.; Kutzer, A.; Göttlicher, J.

    2013-12-01

    A new mineral, kasatkinite, Ba2Ca8B5Si8O32(OH)3 · 6H2O, has been found at the Bazhenovskoe chrysotile asbestos deposit, the Central Urals, Russia in the cavities in rhodingite as a member of two assemblages: (l) on prehnite, with pectolite, calcite, and clinochlore; and (2) on grossular, with diopside and pectolite. Kasatkinite occurs as spherulites or bunches up to 3 mm in size, occasionally combined into crusts. Its individuals are acicular to hair-like, typically split, with a polygonal cross section, up to 0.5 mm (rarely, to 6 mm) in length and to 20 μm in thickness. They consist of numerous misoriented needle-shaped subindividuals up to several dozen μm long and no more than 1 μm thick. Kasatkinite individuals are transparent and colorless; its aggregates are snow white. The luster is vitreous or silky. No cleavage was observed; the fracture is uneven or splintery for aggregates. Individuals are flexible and elastic. The Mohs' hardness is 4-4.5. D meas = 2.95(5), D calc = 2.89 g/cm3. Kasatkinite is optically biaxial (+), α = 1.600(5), β = 1.603(2), γ = 1.626(2), 2 V meas = 30(20)°, 2 V calc = 40°. The IR spectrum is given. The 11B MAS NMR spectrum shows the presence of BO4 in the absence of BO3 groups. The chemical composition of kasatkinite (wt %; electron microprobe, H2O by gas chromatography) is as follows: 0.23 Na2O, 0.57 K2O, 28.94 CaO, 16.79 BaO, 11.57 B2O3, 0.28 Al2O3, 31.63 SiO2, 0.05 F, 9.05 H2O, -0.02 -O=F2; the total is 99.09. The empirical formula (calculated on the basis of O + F = 41 apfu, taking into account the TGA data) is: Na0.11K0.18Ba1.66Ca7.84B5.05Al0.08Si8.00O31.80(OH)3.06F0.04 · 6.10H2O. Kasatkinite is monoclinic, space group P21/ c, P2/ c, or Pc; the unit-cell dimensions are a = 5.745(3), b = 7.238(2), c = 20.79 (1) Å, β = 90.82(5)°, V = 864(1) Å3, Z = 1. The strongest reflections ( d Å- I[ hkl]) in the X-ray powder diffractions pattern are: 5.89-24[012], 3.48-2.1[006], 3.36-24[114]; 3.009-100[, 121, ], 2

  8. Mosaic composition of ribA and wspB genes flanking the virB8-D4 operon in the Wolbachia supergroup B-strain, wStr.

    PubMed

    Baldridge, Gerald D; Li, Yang Grace; Witthuhn, Bruce A; Higgins, LeeAnn; Markowski, Todd W; Baldridge, Abigail S; Fallon, Ann M

    2016-01-01

    The obligate intracellular bacterium, Wolbachia pipientis (Rickettsiales), is a widespread, vertically transmitted endosymbiont of filarial nematodes and arthropods. In insects, Wolbachia modifies reproduction, and in mosquitoes, infection interferes with replication of arboviruses, bacteria and plasmodia. Development of Wolbachia as a tool to control pest insects will be facilitated by an understanding of molecular events that underlie genetic exchange between Wolbachia strains. Here, we used nucleotide sequence, transcriptional and proteomic analyses to evaluate expression levels and establish the mosaic nature of genes flanking the T4SS virB8-D4 operon from wStr, a supergroup B-strain from a planthopper (Hemiptera) that maintains a robust, persistent infection in an Aedes albopictus mosquito cell line. Based on protein abundance, ribA, which contains promoter elements at the 5'-end of the operon, is weakly expressed. The 3'-end of the operon encodes an intact wspB, which encodes an outer membrane protein and is co-transcribed with the vir genes. WspB and vir proteins are expressed at similar, above average abundance levels. In wStr, both ribA and wspB are mosaics of conserved sequence motifs from Wolbachia supergroup A- and B-strains, and wspB is nearly identical to its homolog from wCobU4-2, an A-strain from weevils (Coleoptera). We describe conserved repeated sequence elements that map within or near pseudogene lesions and transitions between A- and B-strain motifs. These studies contribute to ongoing efforts to explore interactions between Wolbachia and its host cell in an in vitro system.

  9. Quantitative Analysis of CF4 Produced in the SiO2 Etching Process Using c-C4F8, C3F8, and C2F6 Plasmas by In Situ Mass Spectrometry

    NASA Astrophysics Data System (ADS)

    Furuya, Kenji; Hatano, Yoshihiko

    2004-01-01

    The use of CF32+ as a specific product ion to selectively quantify CF4 produced in the SiO2 etching process using plasmas of perfluorocompounds (PFCs), such as c-C4F8, C3F8, and C2F6, has been proposed and investigated in the present experiments by measuring mass spectra inside and outside the plasmas. It is known that the CF32+ ion does not appear in the mass spectra of any stable PFCs, except for CF4. It is confirmed in the present experiments that the quantity of CF32+ originating from the CF3 radical in the mass spectra measured in situ is negligible. Other unstable chemical species in the plasmas are too small in quantity to explain the intensity of CF32+ appearing in the mass spectra measured in situ, even if they could produce stable CF32+ by ionization. It is therefore concluded that CF32+ can be used as a fingerprint of CF4 in mass spectrometry. Application of this new method for the quantitative analysis of CF4 produced in the SiO2 etching process using PFC plasmas results in CF4 production advancing significantly not only in the etching region of SiO2 but also in the downstream region of the plasmas.

  10. Alkyl nitrate formation from the reactions of C8-C14 n-alkanes with OH radicals in the presence of NO(x): measured yields with essential corrections for gas-wall partitioning.

    PubMed

    Yeh, Geoffrey K; Ziemann, Paul J

    2014-09-18

    In this study, C8-C14 n-alkanes were reacted with OH radicals in the presence of NO(x) in a Teflon film environmental chamber and isomer-specific yields of alkyl nitrates were determined using gas chromatography. Because results indicated significant losses of alkyl nitrates to chamber walls, gas-wall partitioning was investigated by monitoring the concentrations of a suite of synthesized alkyl nitrates added to the chamber. Gas-to-wall partitioning increased with increasing carbon number and with proximity of the nitrooxy group to the terminal carbon, with losses as high as 86%. The results were used to develop a structure-activity model to predict the effects of carbon number and isomer structure on gas-wall partitioning, which was used to correct the measured yields of alkyl nitrate isomers formed in chamber reactions. The resulting branching ratios for formation of secondary alkyl nitrates were similar for all isomers of a particular carbon number, and average values, which were almost identical to alkyl nitrate yields, were 0.219, 0.206, 0.254, 0.291, and 0.315 for reactions of n-octane, n-decane, n-dodecane, n-tridecane, and n-tetradecane, respectively. The increase in average branching ratios and alkyl nitrate yields with increasing carbon number to a plateau value of ∼0.30 at about C13-C14 is consistent with predictions of a previously developed model, indicating that the model is valid for alkane carbon numbers ≥C3.

  11. Influence of the availability of iron during hypoxia on the genes associated with apoptotic activity and local iron metabolism in rat H9C2 cardiomyocytes and L6G8C5 skeletal myocytes.

    PubMed

    Dziegala, Magdalena; Kasztura, Monika; Kobak, Kamil; Bania, Jacek; Banasiak, Waldemar; Ponikowski, Piotr; Jankowska, Ewa A

    2016-10-01

    The differential availability of iron during hypoxia is presumed to affect the functioning of cardiac and skeletal myocytes. Rat H9C2 cardiomyocytes and L6G8C5 myocytes were cultured for 48 h in normoxic or hypoxic conditions at the optimal, reduced or increased iron concentration. The mRNA expression levels of markers of apoptosis [B‑cell lymphoma‑2 (Bcl2; inhibition) and Bcl‑2‑activated X protein (Bax; induction)], atrophy (Atrogin), glycolysis (pyruvate kinase 2; PKM2) and iron metabolism [transferrin receptor 1 (TfR1; iron importer), ferroportin 1 (FPN1; iron exporter), ferritin heavy chain (FTH; iron storage protein) and hepcidin (HAMP; iron regulator)] were determined using reverse transcription‑quantitative polymerase chain reaction, and cell viability was measured using an tetrazolium reduction assay. Cardiomyocytes and myocytes, when exposed to hypoxia, demonstrated an increased Bax/Bcl‑2 gene expression ratio (P<0.05). Additional deferoxamine (DFO) treatment resulted in further increases in Bax/Bcl‑2 in each cell type (P<0.001 each) and this was associated with the 15% loss in viability. The analogous alterations were observed in both cell types upon ammonium ferric citrate (AFC) treatment during hypoxia; however, the increased Bax/Bcl‑2 ratio and associated viability loss was lower compared with that in case of DFO treatment (P<0.05 each). Under hypoxic conditions, myocytes demonstrated an increased expression of PKM2 (P<0.01). Additional DFO treatment caused an increase in the mRNA expression levels of PKM2 and Atrogin‑1 (P<0.001 and P<0.05, respectively), whereas AFC treatment caused an increased mRNA expression of PKM2 (P<0.01) and accompanied decreased mRNA expression of Atrogin‑1 (P<0.05). The expression augmentation of PKM2 during hypoxia was greater upon low iron compared with that of ferric salt treatment (P<0.01). Both cell types upon DFO during hypoxia demonstrated the increased expression of TfR1

  12. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., counseling, financial aid, physical education, athletics, recreation, transportation, other extracurricular... activities not operated wholly by the recipient as part of, or equivalent to, education programs or activities operated by the recipient shall assure itself that the other education program or activity, as...

  13. 15 CFR 8b.20 - Admission and recruitment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... speaking skills, the test results accurately reflect the applicant's aptitude or achievement level of..., manual, or speaking skills (except where those skills are the factors that the test purports to measure); (ii) admissions tests that are designed for persons with impaired sensory, manual, or speaking...

  14. 15 CFR 8b.20 - Admission and recruitment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... speaking skills, the test results accurately reflect the applicant's aptitude or achievement level of..., manual, or speaking skills (except where those skills are the factors that the test purports to measure); (ii) admissions tests that are designed for persons with impaired sensory, manual, or speaking...

  15. 15 CFR 8b.20 - Admission and recruitment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... speaking skills, the test results accurately reflect the applicant's aptitude or achievement level of..., manual, or speaking skills (except where those skills are the factors that the test purports to measure); (ii) admissions tests that are designed for persons with impaired sensory, manual, or speaking...

  16. 15 CFR 8b.20 - Admission and recruitment.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... speaking skills, the test results accurately reflect the applicant's aptitude or achievement level of..., manual, or speaking skills (except where those skills are the factors that the test purports to measure); (ii) admissions tests that are designed for persons with impaired sensory, manual, or speaking...

  17. 15 CFR 8b.20 - Admission and recruitment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... speaking skills, the test results accurately reflect the applicant's aptitude or achievement level of..., manual, or speaking skills (except where those skills are the factors that the test purports to measure); (ii) admissions tests that are designed for persons with impaired sensory, manual, or speaking...

  18. Crystal structures of the novel hydrated borates Ba{sub 2}B{sub 5}O{sub 9}(OH), Sr{sub 2}B{sub 5}O{sub 9}(OH) and Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2}

    SciTech Connect

    McMillen, Colin; Heyward, Carla; Giesber, Henry; Kolis, Joseph

    2011-11-15

    Three novel hydrated borates Ba{sub 2}B{sub 5}O{sub 9}(OH) (1), Sr{sub 2}B{sub 5}O{sub 9}(OH) (2) and Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2} (3) have been synthesized hydrothermally and their structures determined. Compounds (1) and (2) are isostructural, crystallizing in space group P2{sub 1}/c and having lattice parameters of a=6.6330(13) A, b=8.6250(17) A, c=14.680(3) A, {beta}=93.46(3){sup o} and a=6.4970(13) A, b=8.4180(17) A, c=14.177(3) A, {beta}=94.35(3){sup o}, respectively. Compound (3) crystallizes in P-1 with lattice parameters of a=6.4684(13) A, b=8.4513(17) A, c=14.881(3) A, {alpha}=101.21(3){sup o}, {beta}=93.96(3){sup o}, {gamma}=90.67(3){sup o}. While similar in their axis lengths, (3) differs greatly in structure and formulation from (1) and (2). The structure of (1) and (2) is contrasted to that of the well-known mineral hilgardite (Ca{sub 2}B{sub 5}O{sub 9}Cl.H{sub 2}O). - Graphical abstract: Novel hydrated borate structures. Highlights: > Synthesis and crystal structures of three novel hydrated borates are reported. > Unique descriptive chemistry governing Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2} synthesis is discussed. > Structures of Ba{sub 2}B{sub 5}O{sub 9}(OH) and Sr{sub 2}B{sub 5}O{sub 9}(OH) are contrasted to Ca{sub 2}B{sub 5}O{sub 9}Cl.H{sub 2}O. > Structure of Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2} differs from Ba{sub 2}B{sub 5}O{sub 9}(OH) and Sr{sub 2}B{sub 5}O{sub 9}(OH).

  19. JT8D Engine Internal Exhaust Mixer Technology Program.

    DTIC Science & Technology

    1980-04-01

    fabricability and cost considerations. In addition, this material has excellent repairability properties for service usage. The aluminum fan duct fairing...this period. (3) Along with noise and performance, ambient air temperature, rela- tive humidity, barometric pressure, and wind velocity were re- corded...at each test condition. Neither noise nor performance were recorded when the wind speed exceeded 8 knots or when the crosswind component exceeded 5

  20. 15 CFR 8c.30 - General prohibitions against discrimination.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... providing any aid, benefit, or service, may not, directly or through contractual, licensing, or other... basis of handicap, be excluded from participation in, be denied the benefits of, or otherwise be... participate in or benefit from the aid, benefit, or service; (ii) Afford a qualified individual with...

  1. F-8C adaptive control law refinement and software development

    NASA Technical Reports Server (NTRS)

    Hartmann, G. L.; Stein, G.

    1981-01-01

    An explicit adaptive control algorithm based on maximum likelihood estimation of parameters was designed. To avoid iterative calculations, the algorithm uses parallel channels of Kalman filters operating at fixed locations in parameter space. This algorithm was implemented in NASA/DFRC's Remotely Augmented Vehicle (RAV) facility. Real-time sensor outputs (rate gyro, accelerometer, surface position) are telemetered to a ground computer which sends new gain values to an on-board system. Ground test data and flight records were used to establish design values of noise statistics and to verify the ground-based adaptive software.

  2. 15 CFR 8c.50 - Program accessibility: Existing facilities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... accessibility requirements to the extent compelled by the Architectural Barriers Act of 1968, as amended (42 U.S... changes shall be made by July 26, 1991, but in any event as expeditiously as possible. (d) Transition plan... accessibility, the agency shall develop, by January 26, 1989, a transition plan setting forth the...

  3. Heterocycles [h]-fused onto 4-oxoquinoline-3-carboxylic acid, part VIII [1]. Convenient synthesis and antimicrobial properties of substituted hexahydro[1,4]diazepino[2,3-h]quinoline-9-carboxylic acid and its tetrahydroquino[7,8-b]benzodiazepine analog.

    PubMed

    Al-Hiari, Yusuf M; Abu-Dahab, Rana; El-Abadelah, Mustafa M

    2008-11-18

    [1,4]Diazepino[2,3-h]quinolone carboxylic acid 3 and its benzo-homolog tetrahydroquino[7,8-b]benzodiazepine-3-carboxylic acid 5 were prepared via PPAcatalyzed thermal lactamization of the respective 8-amino-7-substituted-1,4-dihydroquinoline-3-carboxylic acid derivatives 8, 10. The latter compounds were obtained by reduction of their 8-nitro-7-substituted-1,4-dihydroquinoline-3-carboxylic acid precursors 7, 9 which, in turn, were prepared by reaction of 7-chloro-1-cyclopropyl-6-fluoro-8-nitro-1,4-dihydroquinoline-3-carboxylic acid (6) with each of beta-alanine and anthranilic acid. All intermediates and target compounds were characterized using elemental analysis, NMR, IR and MS spectral data. The prepared targets and the intermediates have shown interesting antibacterial activity mainly against Gram positive strains. In particular, compound 8 showed good activity against S. aureus (MIC = 0.39 microg/mL) and B. subtilis (MIC = 0.78 microg/mL). Compounds 5a and 9 have also displayed good antifungal activity against C. albicans (MIC = 1.56 microg/mL and 0.78 microg/mL, respectively). None of the compounds tested showed any anticancer activity against solid breast cancer cell line MCF-7 cells or a human breast adenocarcinoma cell line.

  4. 30 CFR 901.15 - Approval of Alabama regulatory program amendments.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 880-X-2A-.06; 2B-.01; 7B-.07; 7D-.10; 8A-.07; 8B-.06; 8C-.08; 8D-.08, .14; 8E-.05, .06, .10, .11; 8F..., .19; 8I-.12, .17, .19; 8J-.08; 9A-.04; 9B-.04; 9C-.03, (7), .04; 9D-.02; 9E-.05; 10B-.01, 02, .06, .07... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Approval of Alabama regulatory...

  5. 30 CFR 901.15 - Approval of Alabama regulatory program amendments.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 880-X-2A-.06; 2B-.01; 7B-.07; 7D-.10; 8A-.07; 8B-.06; 8C-.08; 8D-.08, .14; 8E-.05, .06, .10, .11; 8F..., .19; 8I-.12, .17, .19; 8J-.08; 9A-.04; 9B-.04; 9C-.03, (7), .04; 9D-.02; 9E-.05; 10B-.01, 02, .06, .07... 30 Mineral Resources 3 2011-07-01 2011-07-01 false Approval of Alabama regulatory...

  6. 30 CFR 901.15 - Approval of Alabama regulatory program amendments.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 880-X-2A-.06; 2B-.01; 7B-.07; 7D-.10; 8A-.07; 8B-.06; 8C-.08; 8D-.08, .14; 8E-.05, .06, .10, .11; 8F..., .19; 8I-.12, .17, .19; 8J-.08; 9A-.04; 9B-.04; 9C-.03, (7), .04; 9D-.02; 9E-.05; 10B-.01, 02, .06, .07... 30 Mineral Resources 3 2012-07-01 2012-07-01 false Approval of Alabama regulatory...

  7. 30 CFR 901.15 - Approval of Alabama regulatory program amendments.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 880-X-2A-.06; 2B-.01; 7B-.07; 7D-.10; 8A-.07; 8B-.06; 8C-.08; 8D-.08, .14; 8E-.05, .06, .10, .11; 8F..., .19; 8I-.12, .17, .19; 8J-.08; 9A-.04; 9B-.04; 9C-.03, (7), .04; 9D-.02; 9E-.05; 10B-.01, 02, .06, .07... 30 Mineral Resources 3 2013-07-01 2013-07-01 false Approval of Alabama regulatory...

  8. 30 CFR 901.15 - Approval of Alabama regulatory program amendments.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 880-X-2A-.06; 2B-.01; 7B-.07; 7D-.10; 8A-.07; 8B-.06; 8C-.08; 8D-.08, .14; 8E-.05, .06, .10, .11; 8F..., .19; 8I-.12, .17, .19; 8J-.08; 9A-.04; 9B-.04; 9C-.03, (7), .04; 9D-.02; 9E-.05; 10B-.01, 02, .06, .07... 30 Mineral Resources 3 2014-07-01 2014-07-01 false Approval of Alabama regulatory...

  9. Shaped Sonic Boom Demonstration/Experiment Airborne Data

    NASA Technical Reports Server (NTRS)

    Haering ,Edward A., Jr.; Murray, James E.

    2004-01-01

    This viewgraph presentation reviews NASA's project to demonstrate that careful design of aircraft contour the resultant sonic boom can maintain a tailored shape, propagating through a real atmosphere down to ground level. The areas in covered in this presentation are: (1) Past airborne shock measurement efforts, (2) SR-71 Sonic Boom Propagation Experiment (3) F-5E Inlet Spillage Shock Measurement (4) Flight test approach (5) GPS data (6) Shaped Sonic Boom Demonstration (SSBD) Mach calibration (7) Super Blanik L-23 sailplane (8) Near-field probing (8a)Maneuvers (8b) Control Room Displays (8c) Pressure Instrumentation (8d) Signatures.

  10. Estimation of the glass forming ability of the Fe-based bulk metallic glass Fe68.8C7.0Si3.5B5.0P9.6Cr2.1Mo2.0Al2.0 that contains non-metallic inclusions

    NASA Astrophysics Data System (ADS)

    Li, Hongxiang; Lu, Zhaoping; Yi, Seonghoon

    2009-02-01

    For the mass production of bulk metallic glasses, the use of industrial raw materials that contain certain amounts of inclusions is inevitable. The glass-forming ability of bulk metallic glasses, i.e., the critical cooling rate for glass formation upon solidification, is closely related to the nature of heterogeneous nucleation offered by inclusions during the solidification process. Significantly different effects of various types of inclusions on the glass forming ability of the alloy Fe68.8C7.0Si3.5B5.0P9.6Cr2.1Mo2.0Al2.0 are demonstrated in this study. The origins of the effects of different inclusions on the glass forming ability are analyzed through thermodynamic, crystallographic and classical heterogeneous nucleation kinetic theories.

  11. A novel technique for measurement of thermal rate constants and temperature dependences of dissociative recombination: CO2+, CF3+, N2O+, C7H8+, C7H7+, C6H6+, C6H5+, C5H6+, C4H4+, and C3H3+

    NASA Astrophysics Data System (ADS)

    Fournier, Joseph A.; Shuman, Nicholas S.; Melko, Joshua J.; Ard, Shaun G.; Viggiano, Albert A.

    2013-04-01

    A novel technique using a flowing afterglow-Langmuir probe apparatus for measurement of temperature dependences of rate constants for dissociative recombination (DR) is presented. Low (˜1011 cm-3) concentrations of a neutral precursor are added to a noble gas/electron afterglow plasma thermalized at 300-500 K. Charge exchange yields one or many cation species, each of which may undergo DR. Relative ion concentrations are monitored at a fixed reaction time while the initial plasma density is varied between 109 and 1010 cm-3. Modeling of the decrease in concentration of each cation relative to the non-recombining noble gas cation yields the rate constant for DR. The technique is applied to several species (O2+, CO2+, CF3+, N2O+) with previously determined 300 K values, showing excellent agreement. The measurements of those species are extended to 500 K, with good agreement to literature values where they exist. Measurements are also made for a range of CnHm+ (C7H7+, C7H8+, C5H6+, C4H4+, C6H5+, C3H3+, and C6H6+) derived from benzene and toluene neutral precursors. CnHm+ DR rate constants vary from 8-12 × 10-7 cm3 s-1 at 300 K with temperature dependences of approximately T-0.7. Where prior measurements exist these results are in agreement, with the exception of C3H3+ where the present results disagree with a previously reported flat temperature dependence.

  12. A novel technique for measurement of thermal rate constants and temperature dependences of dissociative recombination: CO2(+), CF3(+), N2O(+), C7H8(+), C7H7(+), C6H6(+), C6H5(+), C5H6(+), C4H4(+), and C3H3(+).

    PubMed

    Fournier, Joseph A; Shuman, Nicholas S; Melko, Joshua J; Ard, Shaun G; Viggiano, Albert A

    2013-04-21

    A novel technique using a flowing afterglow-Langmuir probe apparatus for measurement of temperature dependences of rate constants for dissociative recombination (DR) is presented. Low (~10(11) cm(-3)) concentrations of a neutral precursor are added to a noble gas∕electron afterglow plasma thermalized at 300-500 K. Charge exchange yields one or many cation species, each of which may undergo DR. Relative ion concentrations are monitored at a fixed reaction time while the initial plasma density is varied between 10(9) and 10(10) cm(-3). Modeling of the decrease in concentration of each cation relative to the non-recombining noble gas cation yields the rate constant for DR. The technique is applied to several species (O2(+), CO2(+), CF3(+), N2O(+)) with previously determined 300 K values, showing excellent agreement. The measurements of those species are extended to 500 K, with good agreement to literature values where they exist. Measurements are also made for a range of CnHm(+) (C7H7(+), C7H8(+), C5H6(+), C4H4(+), C6H5(+), C3H3(+), and C6H6(+)) derived from benzene and toluene neutral precursors. CnHm(+) DR rate constants vary from 8-12 × 10(-7) cm(3) s(-1) at 300 K with temperature dependences of approximately T(-0.7). Where prior measurements exist these results are in agreement, with the exception of C3H3(+) where the present results disagree with a previously reported flat temperature dependence.

  13. Review of Chemical, Biological, Radiological, and Nuclear (CBRN) Terminology in Technical Guide 316 (TG 316) and Allied Medical Publication 8(C) (AMedP-8(C))

    DTIC Science & Technology

    2013-05-01

    Exposure to Specified Biological Agents: Brucellosis , Glanders, Q Fever, SEB and Tularemia, IDA Document D-4132 (Alexandria, VA: Institute for Defense...Biological Agents: Brucellosis , Glanders, Q Fever, SEB and Tularemia. IDA Document D- 4132. Alexandria, VA: IDA, November 2010. Lee, Vernon J. and Mark I

  14. 17 CFR 270.8b-16 - Amendments to registration statement.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... day-to-day management of the company's portfolio (described in Item 9.1.c of Form N-2), including any... responsible for the management of the portfolio. (c) In lieu of including a description of the dividend... statement. (a) Every registered management investment company which is required to file a semi-annual...

  15. AV-8B Map System II: Moving Map Composer Software Users Manual

    DTIC Science & Technology

    2007-11-02

    System Name Location 0 VOODOO NRL, Stennis, MS (old NRL computer; no DMU) 1 MOE NAWC-AD, China Lake, CA 2 LARRY MCAS Yuma, AZ 3 CURLY NRL, Stennis...polygon; (2) entering a series of lat/lon points to define a polygon; (3) using the mouse to draw a "rubber- band " box around a coverage; and (4) picking...The main MMC Window will return. Use the Define Coverage Rectangle button to define a rectangular area of coverage ■—* ; using a rubber- band box

  16. PROCEEDINGS: 1993 SO2 CONTROL SYMPOSIUM - VOLUME 4. SESSIONS 7, 8A, AND 8B

    EPA Science Inventory

    The report documents more than 100 presentations at the 1993 SO2 Control Symposium in Boston, MA, August 24-27, 1993. The presentations covered a wide range of topics: industry's strategies for dealing with Clean Air Act Amendments of 1990, including Phase I strategies, the emiss...

  17. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... and reports shall be in the English language. If any exhibit or other paper or document filed with a registration statement or report is in a foreign language, it shall be accompanied by a translation into the English language. (f) Where a registration statement or report is distributed through an electronic...

  18. Insights into Acetate Toxicity in Zymomonas mobilis 8b using Different Substrates

    DOE PAGES

    Yang, Shihui; Franden, M. A.; Brown, S. D.; ...

    2014-09-30

    The lignocellulosic biomass is a promising renewable feedstock for biofuel production. Acetate is one of the major inhibitors liberated from hemicelluloses during hydrolysis. Likewise, an understanding of the toxic effects of acetate on the fermentation microorganism and the efficient utilization of mixed sugars of glucose and xylose in the presence of hydrolysate inhibitors is crucial for economic biofuel production.

  19. 17 CFR 270.8b-33 - XBRL-Related Documents.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... electronic format as an exhibit to a filing on Form N-1A (§§ 239.15A and 274.11A of this chapter), Form N-CSR... separately identified. A registrant may submit such exhibit with, or in an amendment to, the Form N-CSR...

  20. W8...b4 IM, how did u rite??! Digital Writing in the Composition Classroom

    ERIC Educational Resources Information Center

    Partridge, Bryan

    2011-01-01

    From word processing computers, to mobile telephones, to the advent of the Internet, and finally to online communication venues like Instant Messenger (IM), the past four decades have brought an increasing prevalence of technology into our culture that is altering the English language. While decried by parents and lamented by teachers, these…

  1. International Communique. . . About Information, People, Places, Things. Printing Processes Issue P-8B.

    ERIC Educational Resources Information Center

    Peace Corps, Washington, DC. Information Collection and Exchange Div.

    Focusing on the production and utilization of printing processes in constructing effective visuals for teaching, this bulletin contains articles on the silk screening stencil process, use of a similar process with a portable mimeograph, and the hectograph process. The first article lists equipment needed to make a silk screen, steps in building…

  2. Announcing the Advanced Encryption Standard (AES)

    DTIC Science & Technology

    2007-11-02

    b9 da 5e 15 46 57 a7 8d 9d 84 6 90 d8 ab 00 8c bc d3 0a f7 e4 58 05 b8 b3 45 06 7 d0 2c 1e 8f ca 3f 0f 02 c1 af bd 03 01...e3 4a 46 cf 11 d6 5a d6 5a cf 11 09 63 cf d0 47 fe 7e 88 3 03 ef d2 9a 7b df b5 b8 b8 7b df b5 93 33 7c dc ⊕ 7d 3e 44 3b = 48 67 4d d6 52 85 e3 f6 52...6c fb c8 6c 4f d1 11 3a 4c d1 83 f2 f9 7f 63 35 be d2 fb 96 ae 96 ae d2 fb a9 d1 33 c0 c6 9d b8 15 5 e8 c0 50 01 9b ba 53 7c 7c 9b ba 53 ad 68 8e b0

  3. Identification of Breast Cancer Serum Biomarkers: A Novel Retroviral Library Screen to Define the Breast Cancer-Soluble Ectodomain Proteome

    DTIC Science & Technology

    2009-10-01

    BamHI Not I O P N H A H is Sfi B Age I (2092) HindIII (2568) pBM-Lselectin-HA-IRES-Puro BamHI Not I L-selectin SfiI- B Age I (2092) HindIII (2568) H A...A7 A8 B7 B8 C7 C8 D7 D8 E7 E8 F7 F8 G7 A9 A10 B9 B10 C9 C10 D9 D10 E9 E10 F9 F10 G9 G10 H9 H10 A11 A12 B11 B12C11 C12 D11

  4. Isomerization of the diphosphine ligand 3,4-bis(diphenylphosphino)-5-methoxy-2(5H)-furanone (bmf) at a triosmium cluster and P C bond cleavage in the unsaturated cluster 1,1-Os3(CO)9(bmf): Synthesis and X-ray diffraction structures of the isomeric Os3(CO)10(bmf) clusters and HOs3(CO)8( -C6H4)[ -PhPCC(Ph2P)CH(OMe)OC(O)

    SciTech Connect

    Kandala, Srikanth; Yang, Li; Campana, Charles F.; Nesterov, Vladimir; Richmond, Michael G.

    2010-07-01

    The labile cluster 1,2-Os3(CO)10(MeCN)2 (1) reacts with the chiral diphosphine ligand 3,4-bis(diphenylphosphino)-5-methoxy-2(5H)-furanone (bmf) to furnish 1,2-Os3(CO)10(bmf) (2a) in high yield. Heating cluster 2a over the temperature range 358 383 K under CO leads to isomerization of the bmf ligand and formation of the diphosphine-chelated cluster 1,1-Os3(CO)10(bmf) (2b) and an equilibrium mixture consisting of 2a and 2b in a 15:85 ratio. Extended thermolysis of an equilibrium mixture of Os3(CO)10(bmf) is accompanied by CO loss and ortho-metalation of an aryl ring to afford an inseparable mixture of three diastereomeric hydride clusters HOs3(CO)9(C29H23O3P2) (3a c). Thermolysis of HOs3(CO)9(C29H23O3P2) (3a c) in refluxing toluene leads to P C bond cleavage and formation of the benzyne-substituted clusters HOs3(CO)8( -C6H4)( -C23H19O3P2) (4a,b) as a 4:1 mixture of diastereomers. The unequivocal identity of the major benzyne-substituted cluster has been determined by X-ray diffraction analysis, where the activation of one of the phenyl groups situated to the furanone carbonyl group in the bmf ligand has been established. The isomerization and activation of the bmf ligand are contrasted with other Os3(CO)10(diphosphine) derivatives prepared by our groups.

  5. DC-9 flight demonstration program with refanned JT8D engines. Volume 2: Design and construction

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The nacelle configuration selected for the DC-9 had a 1595.6 mm Refan length inlet and an 1811.8 mm exhaust duct. The inlet had 1234.4 mm of acoustic treatment and the tailpipe had 1305.5 mm of equivalent length acoustic treatment. The pylon was reduced in width from 425.5 mm to 204.5 mm. Fuselage frames and titanium skin panels in the area of the pylon were reinforced or replaced to support the higher loads and engine thrust. Experimental type tooling, fabrication and assembly were used on all hardware. The design is considered certifiable and representative of the hardware that would be built as retrofit kits.

  6. Internal Mixer Investigation for JT8D Engine Jet Noise Reduction. Volume 1 Results.

    DTIC Science & Technology

    1977-12-01

    were compared with~ re- suilts from other con tigurations. Scale Mo1 del Peru rmanc Tests Tlwo mixer". and the rt ference sytem were tested by thle...PN. curves in Vi,. uro 5.4’. Tite slort mixcr peroduccd about I P"NO1 1110K. 10is re. duction Mhan did the longs mixert &tInjakienwi takeoff and

  7. Spectral Re-Growth Reduction for CCSDS 8-D 8-PSK TCM

    NASA Technical Reports Server (NTRS)

    Borah, Deva K.

    2002-01-01

    This report presents a study on the CCSDS recommended 8-dimensional 8 PSK Trellis Coded Modulation (TCM) scheme. The important steps of the CCSDS scheme include: conversion of serial data into parallel form, differential encoding, convolutional encoding, constellation mapping, and filtering the 8-PSK symbols using the square root raised cosine (SRRC) pulses. The last step, namely the filtering of the 8 PSK symbols using SRRC pulses, significantly affects the bandwidth of the signal. If a nonlinear power amplifier is used, the SRRC filtered signal creates spectral regrowth. The purpose of this report is to investigate a technique, called the smooth phase interpolated keying (SPIK), that can provide an alternative to SRRC filtering so that good spectral as well as power efficiencies can be obtained with the CCSDS encoder. The results of this study show that the CCSDS encoder does not affect the spectral shape of the SRRC filtered signal or the SPIK signal. When a nonlinear traveling wave tube amplifier (TWTA) is used, the spectral performance of the SRRC signal degrades significantly while the spectral performance of SPIK remains unaffected. The degrading effect of a nonlinear solid state power amplifier (SSPA) on SRRC is found to be less than that due to a nonlinear TWTA. However, in both cases, the spectral performance of the SRRC modulated signal is worse than that of the SPIK signal. The bit error rate (BER) performance of the SRRC signal in a linear amplifier environment is about 2.5 dB better than that of the SPIK signal when both the receivers use algorithms of similar complexity. In a nonlinear TWTA environment, the SRRC signal requires accurate phase tracking since the TWTA introduces additional phase distortion. This problem does not arise with SPIK signal due to its constant envelope property. When a nonlinear amplifier is used, the SRRC method loses nearly 1 dB in the bit error rate performance. The SPIK signal does not lose any performance. Thus the performance gap between SRRC and SPIK reduces. The BER performance of SPIK can be improved even further by using a more optimal receiver. A similar optimal receiver for SRRC is quite complex since the amplifier distorts the pulse shape. However, this requires further investigation and is not covered in this report.

  8. 8D likelihood effective Higgs couplings extraction framework in h → 4ℓ

    DOE PAGES

    Chen, Yi; Di Marco, Emanuele; Lykken, Joe; ...

    2015-01-23

    We present an overview of a comprehensive analysis framework aimed at performing direct extraction of all possible effective Higgs couplings to neutral electroweak gauge bosons in the decay to electrons and muons, the so called ‘golden channel’. Our framework is based primarily on a maximum likelihood method constructed from analytic expressions of the fully differential cross sections for h → 4l and for the dominant irreduciblemore » $$ q\\overline{q} $$ → 4l background, where 4l = 2e2μ, 4e, 4μ. Detector effects are included by an explicit convolution of these analytic expressions with the appropriate transfer function over all center of mass variables. Utilizing the full set of observables, we construct an unbinned detector-level likelihood which is continuous in the effective couplings. We consider possible ZZ, Zγ, and γγ couplings simultaneously, allowing for general CP odd/even admixtures. A broad overview is given of how the convolution is performed and we discuss the principles and theoretical basis of the framework. This framework can be used in a variety of ways to study Higgs couplings in the golden channel using data obtained at the LHC and other future colliders.« less

  9. 8D likelihood effective Higgs couplings extraction framework in h → 4ℓ

    SciTech Connect

    Chen, Yi; Di Marco, Emanuele; Lykken, Joe; Spiropulu, Maria; Vega-Morales, Roberto; Xie, Si

    2015-01-23

    We present an overview of a comprehensive analysis framework aimed at performing direct extraction of all possible effective Higgs couplings to neutral electroweak gauge bosons in the decay to electrons and muons, the so called ‘golden channel’. Our framework is based primarily on a maximum likelihood method constructed from analytic expressions of the fully differential cross sections for h → 4l and for the dominant irreducible $ q\\overline{q} $ → 4l background, where 4l = 2e2μ, 4e, 4μ. Detector effects are included by an explicit convolution of these analytic expressions with the appropriate transfer function over all center of mass variables. Utilizing the full set of observables, we construct an unbinned detector-level likelihood which is continuous in the effective couplings. We consider possible ZZ, Zγ, and γγ couplings simultaneously, allowing for general CP odd/even admixtures. A broad overview is given of how the convolution is performed and we discuss the principles and theoretical basis of the framework. This framework can be used in a variety of ways to study Higgs couplings in the golden channel using data obtained at the LHC and other future colliders.

  10. Technical Note for 8D Likelihood Effective Higgs Couplings Extraction Framework in the Golden Channel

    SciTech Connect

    Chen, Yi; Di Marco, Emanuele; Lykken, Joe; Spiropulu, Maria; Vega-Morales, Roberto; Xie, Si

    2014-10-17

    In this technical note we present technical details on various aspects of the framework introduced in arXiv:1401.2077 aimed at extracting effective Higgs couplings in the $h\\to 4\\ell$ `golden channel'. Since it is the primary feature of the framework, we focus in particular on the convolution integral which takes us from `truth' level to `detector' level and the numerical and analytic techniques used to obtain it. We also briefly discuss other aspects of the framework.

  11. Evaluation of a Gentex (registered trademark) ORO-NASAL Oxygen Mask for Integration with the Aqualung (registered trademark) Personal Helicopter Oxygen Delivery System (PHODS)

    DTIC Science & Technology

    2008-07-13

    13 8b. Onyx II® Digital Pulse Oximeter...was backed up with an Onyx II pulse oximeter (figure 8b) (Yamaya et al., 2002). Additionally, color vision testing using the Ishihara pseudo...Figure 8b. Onyx II® Digital Pulse Oximeter. 14 Figure 8c. Blue

  12. 17 CFR 240.8c-1 - Hypothecation of customers' securities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... derivative instrument, or pursuant to the OTC derivatives dealer's cash management securities activities or ancillary portfolio management securities activities, and who has received a prominent written notice...

  13. 17 CFR 240.8c-1 - Hypothecation of customers' securities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... derivative instrument, or pursuant to the OTC derivatives dealer's cash management securities activities or ancillary portfolio management securities activities, and who has received a prominent written notice...

  14. Environmental Assessment for North Central Texas Relay Node Site NO. RN8C915TX

    DTIC Science & Technology

    1993-02-11

    River drainage basins (Davenport, 1990). The central and southern portions of the SSA drain southward into the Brazos River (Jones, 1990; Sims, 1989...guy anchors and to prevent inadvertent exposure to electric shock resulting from the buildup of static electric charge. In addition to the main tower...area in Knox and Baylor counties centered 3.6 miles south of the town of Vera, in the Southern Great Plains portion of the Central Lowlands

  15. F-8C adaptive flight control extensions. [for maximum likelihood estimation

    NASA Technical Reports Server (NTRS)

    Stein, G.; Hartmann, G. L.

    1977-01-01

    An adaptive concept which combines gain-scheduled control laws with explicit maximum likelihood estimation (MLE) identification to provide the scheduling values is described. The MLE algorithm was improved by incorporating attitude data, estimating gust statistics for setting filter gains, and improving parameter tracking during changing flight conditions. A lateral MLE algorithm was designed to improve true air speed and angle of attack estimates during lateral maneuvers. Relationships between the pitch axis sensors inherent in the MLE design were examined and used for sensor failure detection. Design details and simulation performance are presented for each of the three areas investigated.

  16. Materials Data on H8C3SeN2 (SG:61) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-02-11

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  17. Materials Data on H8C3SN2 (SG:61) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-02-11

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  18. Environmental Assessment for North Central Wisconsin Relay Node Site No. RN 8C936WI

    DTIC Science & Technology

    1993-02-26

    SCS) as glossoboralfs and glossaqualfs and were formerly designated as grey -brown podzolics. These soils erode easily when exposed, although they are...The WDNR indicated that another federally listed species, the grey or timber wolf, might pass through the area (Vaneck, 1989). The grey wolf is...eagle is 2 miles from the closest CGS. Wolves introduced into Lincoln County are not expected to occur near any of the CGSs, and no impact is expected

  19. Documentation of the Range 8C rehabilitation demonstration project at Hohenfels Training Area, West Germany

    SciTech Connect

    Zellmer, S.D.; Hinchman, R.R.; Carter, R.P.; Severinghaus, W.D.; Lacey, R.M.; Brent, J.J.

    1987-03-01

    Continued and intensive tactical training for the last 35 years at the Hohenfels Training Area (HTA), Federal Republic of Germany, has resulted in extensive environmental damage and reduced training realism. The US Corps of Engineers Construction Engineering Research Laboratory is developing an Integrated Training Area Management (ITAM) Program for the Seventh Army Training Command for use at HTA. Argonne National Laboratory was asked to assist in one element of the ITAM program, a training range rehabilitation demonstration project. The rehabilitation project was begun in 1986 on a 62-ha watershed that included about 16 ha of meadow with training damage typical of HTA. On the basis of amount of plant ground cover, type and degree of erosion, and soil properties, 10 rehabilitation prescriptions were developed to reestablish plant cover, control erosion, and improve training realism. Prescriptions were installed by a local contractor in September 1986. A monitoring program is under way to determine the effectiveness of this effort. Results and experience gained from this project will be used in the ITAM program and for rehabilitation training courses conducted at HTA.

  20. Materials Data on H8C3(NO)4 (SG:14) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-02-04

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  1. Operation Dominic, Fish Bowl Series. Project Officer’s Report. Project 8C

    DTIC Science & Technology

    1963-07-03

    1.2 BACKGROUND AND THEOR I0 J$ Gaseous or solid explouives bave been applied d ctly to full- scale vebioles to simulate structural responses= ib e...with the real case as structural responses. Thus, during the past 3 years# a large- scale theoret- losl and ezperiasntal simlation program e" been...separation froz th~e Thor was monitcoed by inc1i4ine two, sepuirstion ( switches in tlio spacer assemb3yv. One separation sirnal. C ve & traneuittod via Thor

  2. Environmental Assessment for Southern Utah Relay Node Site NO. RN 8C919UT

    DTIC Science & Technology

    1993-03-18

    3-6 The SSA contains no national or state parks, refuges, preserves , or sanctuaries (USFWS, 1986). The nearest preserve is the Grand Canyon Game... Preserve , located 0.5 mile southeast of the SSA. In compliance with Section 7 of the Endangered Species Act of 1973, as amended (16 USC 1531, et seq., at...Utah (Durham, 1990). As required by the National Historic Preservation Act (16 USC 470, et seq.), the Arizona and Utah State Historic Preservation

  3. Materials Data on H8C2NCl (SG:72) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-02-11

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  4. Materials Data on H8C3SN(OF)3 (SG:14) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-04-22

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  5. Materials Data on H8C3S2 (SG:14) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-02-11

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  6. Materials Data on H8C3O2 (SG:14) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-02-11

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  7. γ-Hydroxyethyl piperidine iminosugar and N-alkylated derivatives: a study of their activity as glycosidase inhibitors and as immunosuppressive agents.

    PubMed

    Markad, Pramod R; Sonawane, Dhiraj P; Ghosh, Sougata; Chopade, Balu A; Kumbhar, Navnath; Louat, Thierry; Herman, Jean; Waer, Mark; Herdewijn, Piet; Dhavale, Dilip D

    2014-11-01

    An efficient and practical strategy for the synthesis of (3R,4s,5S)-4-(2-hydroxyethyl) piperidine-3,4,5-triol and its N-alkyl derivatives 8a-f, starting from the D-glucose, is reported. The chiral pool methodology involves preparation of the C-3-allyl-α-D-ribofuranodialdose 10, which was converted to the C-5-amino derivative 11 by reductive amination. The presence of C-3-allyl group gives an easy access to the requisite hydroxyethyl substituted compound 13. Intramolecular reductive aminocyclization of C-5 amino group with C-1 aldehyde provided the γ-hydroxyethyl substituted piperidine iminosugar 8a that was N-alkylated to get N-alkyl derivatives 8b-f. Iminosugars 8a-f were screened against glycosidase enzymes. Amongst synthetic N-alkylated iminosugars, 8b and 8c were found to be α-galactosidase inhibitors while 8d and 8e were selective and moderate α-mannosidase inhibitors. In addition, immunomodulatory activity of compounds 8a-f was examined. These results were substantiated by molecular docking studies using AUTODOCK 4.2 programme.

  8. A 3.8 b.y. History of Bacterial Biofilms and Their Significance in the Search for Extraterrestrial Life

    NASA Technical Reports Server (NTRS)

    Westall, Frances; Steele, Andrew; Toporski, Jan; Walsh, Maud; Allen, Carlton; Guidry, Sean; McKay, David; Gibson, Everett; Chafetz, Henry

    2000-01-01

    Bacterial biofilms are almost ubiquitous in terrestrial environments, many similar to past or present Martian environments. Together with ToF-SIMS analysis of the in situ organics, fossil biofilms constitute reliable biomarkers.

  9. SEMICONDUCTOR INTEGRATED CIRCUITS: An 8-b 300MS/S folding and interpolating ADC for embedded applications

    NASA Astrophysics Data System (ADS)

    Yan, Lu; Li, Lin; Jiefeng, Xia; Fan, Ye; Junyan, Ren

    2010-06-01

    A 1.4-V 8-bit 300-MS/s folding and interpolating analog-to-digital converter (ADC) is proposed. Fabricated in the 0.13-μm CMOS process and occupying only 0.6-mm2 active area, the ADC is especially suitable for embedded applications. The system is optimized for a low-power purpose. Pipelining sampling switches help to cut down the extra power needed for complete settling. An averaging resistor array is placed between two folding stages for power-saving considerations. The converter achieves 43.4-dB signal-to-noise and distortion ratio and 53.3-dB spurious-free dynamic range at 1-MHz input and 42.1-dB and 49.5-dB for Nyquist input. Measured results show a power dissipation of 34 mW and a figure of merit of 1.14 pJ/convstep at 250-MHz sampling rate at 1.4-V supply.

  10. How Accurately Do We Know the Cross Section of the 7Be(p, {gamma})8B Reaction?

    SciTech Connect

    Gai, Moshe

    2006-03-13

    The 'world average' of the astrophysical cross section factor , S17(0), is driven by the Seattle result due to the very small quoted uncertainty, which we however demonstrate it to be an overestimated accuracy. We propose more realistic error bars for the Seattle results based on the published Seattle data. This leads to a an uncertainty of the measured slope of S17 and thus an uncertainty due to extrapolation that can be reasonably estimated to be {sub -3.0}{sup +0.0} eV-b.

  11. Documentation of Production: Allied Medical Publication 8(B), Volume 2, Medical Planning Guide of NBC Battle Casualties (Biological)

    DTIC Science & Technology

    2006-06-01

    Figure IV-2. Casualty and Area Coverage Values for STI Data Set .................................. 39 Figure IV-3. Extract from GRIDGEN Special Inputs...data, delivery system and munitions data, and meteorological data. VLSTRACK ( Gridgen ) Attack Point Meteorological Data Biostrike Agent Cloud Exposure...reported in a specific format; to streamline the methodology, IDA developed a variant of VLSTRACK, called Gridgen , to report the outputs of VLSTRACK in a

  12. Materials Data on Zn8B4H3O15F (SG:4) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-02-04

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  13. PoroTomo Subtask 6.8b - Raw Pressure Data from Observation Wells at Brady's Hot Springs

    DOE Data Explorer

    David Lim

    2016-03-13

    This .csv files contain the raw water pressure data from three observation wells during pumping tests performed in the Spring of 2016. Included is a "read me" file explaining the details of where and how the data were collected.

  14. 76 FR 16526 - Airworthiness Directives; Pratt & Whitney JT8D-209, -217, -217A, -217C, and -219 Series Turbofan...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-24

    ... with bolts and nuts made of Tinidur material. This new AD requires the same torque inspection, blade... longer bolts made of Tinidur material. This AD also requires installation of crushable sleeve spacers on the bolts. This AD was prompted by nine reports of failure of Tinidur material LPT-to-exhaust...

  15. 75 FR 38052 - Airworthiness Directives; Pratt & Whitney JT8D-209, -217, -217A, -217C, and -219 Series Turbofan...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-01

    ... and nuts made of Tinidur material. This proposed AD would require the same actions but would require replacement of the LPT-to-exhaust case bolts and nuts with longer bolts made of Tinidur material, with nuts made of Tinidur material, and installation of crushable sleeve spacers on the bolts. This proposed...

  16. PoroTomo Subtask 3.8D - Raw Pressure Data from Boise Hydrogephysical Research Site (BHRS)

    DOE Data Explorer

    Lim, David

    2013-07-17

    Pressure data from a phreatic aquifer was collected in the summer of 2013 during Multi-frequency Oscillatory Hydraulic Tomography pumping tests. All tests were performed at the Boise Hydrogeophysical Research Site. The data will be inverted using a fast steady-periodic adjoint-based inverse code.

  17. Vanishing of phase coherence in underdopedBi_2Sr_2CaCu_2O_8+d

    SciTech Connect

    Corson, John; Mallozzi, R.; Orenstein, J.; Eckstein, N.; Bozovic,I.

    1998-10-21

    Although the binding of electrons into Cooper pairs is essential in forming the superconducting state, its remarkable properties--zero resistance and perfect diamagnetism--require phase coherence among the pairs as well. When coherence is lost at the transition temperature Tc, pairing remains, together with phase correlations which are finite in space and time. In conventional metals, Cooper pairs with short-range phase coherence survive no more than 1 K above Tc. In underdoped high-Tc copper oxides, spectroscopic evidence for some form of pairing is found up to a temperature T*, which is roughly 100 K above Tc (refs 1,2,3). How this pairing and Cooper-pair formation are related is a central problem in high-Tc superconductivity. The nature of this relationship hinges on the extent to which phase correlations accompany pairing in the normal state4. Here we report measurements of high-frequency conductivity that track the phase-correlation time tau in the normal state of the Bi2Sr2CaCu2O8+delta family of underdoped copper oxide superconductors. Just above Tc, we find that tau reflects the motion of thermally generated topological defects in the phase, or vortices5,6. However, vortex proliferation reduces tau to a value indistinguishable from the lifetime of normal-state electrons at 100 K, well below T*.

  18. Corrosion monitoring of high-level waste storage Tank 8-D2 at the West Valley Demonstration Project

    SciTech Connect

    Shukla, R.K.; Bourgeois, P.M.; Jaramins, R.J.; Secen, W.G.; Stroud, D.J.; Perkins, A.J.

    1994-12-31

    From 1966 to 1972, nearly 600,000 gallons of highly radioactive liquid waste were generated at the site of the only commercial nuclear fuel reprocessing facility to have operated in the United States, located in West Valley, New York. This waste has been held in underground storage tanks since reprocessing operations ceased in 1972. Premature failure of tank walls represents a significant safety risk at West Valley. For this reason, application of conventional methods of probe insertion and data acquisition are impractical. Because a satisfactory corrosion monitoring system must allow for remote monitoring, as well as decontamination of any probe or coupon that comes into contact with the liquid high-level waste (HLW) held in the tanks, a fully automated Integrated Corrosion Monitoring System has been implemented at West Valley. This system allows for remote continuous monitoring of corrosion effects by linear polarization and electrical resistance probes. Real time corrosion data that indicates metal loss versus time and corrosion rate for electrical resistance probes, as well as corrosion rate and pitting tendencies for linear polarization resistance probes is depicted on a electroluminescent display. Because corrosion rates are being continuously checked, this system is ideal for monitoring the corrosive conditions that will affect the life of the containment HLW storage vessels. The probes in the system also permits the simultaneous use of coupons for determining specific types of corrosion occurring, and the average corrosion rate for the entire exposure period. This paper describes the design parameters, installation procedures, and the results of data collected from the Integrated Corrosion Monitoring System installed at West Valley.

  19. Materials Data on ZnH8C2N4(ClO)2 (SG:2) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  20. Materials Data on CrH8C4(S2N3)2 (SG:2) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-03-28

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  1. Materials Data on FeH8C4(S2N3)2 (SG:2) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-04-23

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  2. Materials Data on CdH8C4(S2N3)2 (SG:2) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-03-28

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  3. Materials Data on SiH8C2NClO (SG:2) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-02-11

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  4. Materials Data on Sb4H8C2S7N (SG:64) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-02-04

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  5. Materials Data on CdH8C2Br3N (SG:14) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-02-11

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  6. Materials Data on HgH8C2Br3N (SG:11) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-02-11

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  7. 2015 Review on the Extension of the AMedP-8(C) Methodology to New Agents, Materials, and Conditions

    DTIC Science & Technology

    2016-06-01

    Defense Planning & Response Project,” for the Joint Staff, Joint Requirements Office (JRO) for Chemical , Biological, Radiological and Nuclear (CBRN...Treaty Organization (NATO) planning guide documenting a methodology to estimate casualties from chemical , biological, radiological, and nuclear (CBRN...March 2011, included the parameters to estimate casualties caused by three chemical agents, five biological agents, seven radioisotopes, nuclear

  8. 2012 Review on the Extension of the AMedP-8(C) Methodology to New Agents, Materials, and Conditions

    DTIC Science & Technology

    2013-10-01

    technology.com/projects/bavarian_nordic/. 191 Bertram L. Jacobs et al., “Vaccinia Virus Vaccines: Past, Present and Future,” Antiviral Research 84...Countermeasure Screening in Accordance with the FDA Animal Rule.” Health Physics 103, no. 4 (Oct 2012): 463–473. Jacobs, Bertram L., Jeffrey O. Langland

  9. 2013 Review on the Extension of the AMedP-8(C) Methodology to New Agents, Materials, and Conditions

    DTIC Science & Technology

    2014-06-30

    12 Teske et al., “Animal and Human Dose-Response Models for Brucella Species,” Risk Analysis 31 (2011...Dose-Response Model of Coxiella burnetii (Q Fever).” Risk Analysis 31, no. 1 (2011): 120–128. Teske , S. S., Huang, Y., Tamrakar, S. B., Bartrand, T

  10. Materials Data on ZnH8C2(SN3)2 (SG:14) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-04-22

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  11. 2014 Review on the Extension of the AMedP-8(C) Methodology to New Agents, Materials, and Conditions

    DTIC Science & Technology

    2015-08-01

    counterforce tool that predicts the effects of hazardous material releases (nuclear, biological, and chemical) into the atmosphere and their...Consequence Assessment of Atmospheric Releases of Hazardous Materials ,” International Journal of Environment and Pollution 55, no. 1/2/3/4 (2014): 3– 12...Atmospheric Releases of Hazardous Materials . International Journal of Environment and Pollution. 55, no. 1/2/3/4 (2014): 3–12. Rodriquez, Jason

  12. G6PD haplotypes spanning Xq28 from F8C to red/green color vision

    SciTech Connect

    Filosa, S.; Lania, G.; Martini, G. ); Brancati, C.; Tagarelli, A. ); Calabro, V. Hammersmith Hospital, London ); Vulliamy, T.J.; Luzzatto, L. )

    1993-07-01

    The most telomeric region of the human X chromosome within band Xq28 consists of a gene-rich region of about 3 Mb which contains the genes for coagulation factor VIIIc, glucose-6-phosphate dehydrogenase (G6PD), and red/green color vision. The authors have studied five polymorphic sites from this region, in a sample of normal people from the Cosenza province of Southern Italy. These sites, which span a distance of some 350 kb, are in strong linkage disequilibrium. Of the 32 possible haplotypes only 10 were found, and 4 of these account for 80% of all X chromosomes analyzed. In addition, they found that all G6PD-deficient people with the G6PD Mediterranean mutation belong to only two haplotypes. One of these (Med 1) is found only within a small subregion of the area investigated, west of the Appennine mountain range. Most remarkably, all Med 1 G6PD-deficient individuals also had red/green color blindness. The more frequent haplotype (Med 2) is the same in Calabria and in Sardinia, where it accounts for about 90% of the G6PD Mediterranean mutations, despite the fact that gene flow between the populations of Sardinia and Southern Italy must have been limited. These data do not enable determination of whether the two types of G6PD Mediterranean have arisen through two separate identical mutational events or through a single mutational event followed by recombination. However, the data indicate relatively little recombination over an extended region of the X chromosome and they suggest that the G6PD Mediterranean mutation is recent by comparison to the other polymorphisms investigated. 44 refs., 4 figs., 5 tabs.

  13. 2010 Review on the Extension of the AMedP-8(C) Methodology to New Agents, Materials, and Conditions

    DTIC Science & Technology

    2010-12-01

    pneumonic plague, smallpox, and Venezuelan equine encephalitis (VEE)). D. Human Response Injury Profile (HRIP) Parameters The HRIP methodology...would be a significantly different response due to the use of antibiotics (as chemoprophylaxis) or immunizations, and a separate set of prophylaxis...vaccination protocols or existing vaccination research programs and for bacterial agents that respond to antibiotics . E. The 2009 Report and

  14. University Opinion Poll 8C, Fall 1973. Research Bulletin, University of Minnesota, V14 N8 January 28, 1974.

    ERIC Educational Resources Information Center

    Barnhart, William

    The University Opinion Poll conducted a survey to obtain an estimate of student and staff opinion toward alcohol on campus. Four hundred seventy students and staff, 77% of a random sample of all students and staff at the University of Minnesota, were contacted for their opinions. Most respondents favor having alcoholic beverages on campus and…

  15. Materials Data on H8C2SeS2(N2Cl)2 (SG:14) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-04-22

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  16. Cobalt complexes with cinchonidine and quinidine: effect of C8/C9 stereochemistry and 6'-substitution on intermolecular interactions.

    PubMed

    Skórska, Agnieszka; Stadnicka, Katarzyna; Oleksyn, Barbara J

    2005-02-01

    The title compounds, (cinchonidinium)trichlorocobalt(II) [(C(19)H(23)ON(2))CoCl(3)] (CoCdn) and (quinidinium)trichlorocobalt(II) [(C(20)H(25)O(2)N(2))CoCl(3)] (CoQd), are zwitterions that differ in absolute configuration and conformation. In both complexes, the sp(3) nitrogen of quinuclidine is protonated, whereas the sp(2) nitrogen of quinoline is linked to the Co(II) atom, which coordinates three chlorine atoms in distorted tetrahedral geometry. The mutual orientations of the quinoline and quinuclidine moieties in CoCdn and CoQd differ significantly because of different hydrogen bonding involving the hydroxyl group. In both complexes, the quinuclidine NH groups and hydroxyl groups are hydrogen-bond donors to the chlorine atoms of Co(II) tetrahedra. In CoQd the hydrogen bonding leads to formation of a nine-membered ring consisting of Co, two chlorines, and a fragment of the quinidine molecule. A comparison of the crystal structures of four Cinchona alkaloid complexes with trichlorocobalt(II) shows that their space groups are determined by the absolute configuration of the alkaloid, whereas the hydrogen-bonding pattern is mainly affected by the substituent in the quinoline ring, i.e., by hydrogen or methoxyl group.

  17. Claudin-8 and -27 tight junction proteins in puffer fish Tetraodon nigroviridis acclimated to freshwater and seawater.

    PubMed

    Bagherie-Lachidan, Mazdak; Wright, Stephen I; Kelly, Scott P

    2009-05-01

    Genes encoding for claudin-8 and -27 tight junction proteins in the euryhaline puffer fish (Tetraodon nigroviridis) were identified using its recently sequenced genome. Phylogenetic analysis indicated that multiple genes encoding for claudin-8 proteins (designated Tncldn8a, Tncldn8b, Tncldn8c and Tncldn8d) arose by tandem gene duplication. In contrast, both tandem and whole genome duplication events appear to have generated genes encoding for claudin-27 proteins (designated Tncldn27a, Tncldn27b, Tncldn27c and Tncldn27d). Tncldn8 and Tncldn27 mRNA were widely distributed in Tetraodon, suggesting involvement in various physiological processes. All Tncldn8 and Tncldn27 genes were expressed in gill and skin tissue (i.e., epithelia exposed directly to the external environment). A potential role for claudin-8 and -27 proteins in the regulation of hydromineral balance in Tetraodon was investigated by examining alterations in mRNA abundance in select ionoregulatory tissue of fish acclimated to freshwater (FW) and seawater (SW). In FW or SW, Tetraodon exhibited alterations in Na(+)-K(+)-ATPase activity (a correlate of transcellular transport) typical of a euryhaline teleost fish. Simultaneously, tissue and gene specific alterations in Tncldn8 and Tncldn27 transcript abundance occurred. These data provide some insight into the duplication history of cldn8 and cldn27 genes in fishes and suggest a possible role for claudin-8 and -27 proteins in the osmoregulatory strategies of euryhaline teleosts.

  18. The development of an experimental model of subretinal neovascularization in disciform macular degeneration.

    PubMed Central

    Ryan, S J

    1979-01-01

    A reproducible model of subretinal neovascularization has been developed, an important first step in the study of disciform macular degeneration. The methodology and clinical description of subretinal neovascularization in the primate have been provided and histopathologic and electron microscopic correlations included. This model provides a most promising and useful mechanism for the evaluation of the many different factors and hypotheses that have been proposed for the genesis of the disciform response. In humans, it probably has more relevance to the traumatically induced disciform process, particularly that after laser therapy, than to the senile degenerative process. The purpose of this investigation is to develop the animal model by which many different theories regarding the pathogenesis of the disciform response can be tested. These initial studies relate to ischemia, hemorrhage, the inflammatory response, and position in relation to the center of the vascular-free zone as factors important in the development and evolution of subretinal neovascularization. Images FIGURE 1 FIGURE 2 A FIGURE 2 B FIGURE 2 C FIGURE 2 D FIGURE 3 A FIGURE 3 B FIGURE 3 C FIGURE 3 D FIGURE 3 E FIGURE 4 A FIGURE 4 B FIGURE 7 A FIGURE 7 B FIGURE 7 C FIGURE 7 D FIGURE 7 E FIGURE 7 F FIGURE 8 A FIGURE 8 B FIGURE 8 C FIGURE 8 D FIGURE 8 E FIGURE 8 F FIGURE 8 G FIGURE 9 A FIGURE 9 B FIGURE 9 C FIGURE 9 D FIGURE 10 A FIGURE 10 B FIGURE 10 C PMID:94717

  19. Antimutagenic Compounds of White Shrimp (Litopenaeus vannamei): Isolation and Structural Elucidation.

    PubMed

    López-Saiz, Carmen-María; Hernández, Javier; Cinco-Moroyoqui, Francisco-Javier; Velázquez, Carlos; Ocaño-Higuera, Víctor-Manuel; Plascencia-Jatomea, Maribel; Robles-Sánchez, Maribel; Machi-Lara, Lorena; Burgos-Hernández, Armando

    2016-01-01

    According to the World Health Organization, cancer is the main cause of mortality worldwide; thus, the search of chemopreventive compounds to prevent the disease has become a priority. White shrimp (Litopenaeus vannamei) has been reported as a source of compounds with chemopreventive activities. In this study, shrimp lipids were extracted and then fractionated in order to isolate those compounds responsible for the antimutagenic activity. The antimutagenic activity was assessed by the inhibition of the mutagenic effect of aflatoxin B1 on TA98 and TA100 Salmonella tester strains using the Ames test. Methanolic fraction was responsible for the highest antimutagenic activity (95.6 and 95.9% for TA98 and TA100, resp.) and was further separated into fifteen different subfractions (M1-M15). Fraction M8 exerted the highest inhibition of AFB1 mutation (96.5 and 101.6% for TA98 and TA100, resp.) and, after further fractionation, four subfractions M8a, M8b, M8c, and M8d were obtained. Data from (1)H and (13)C NMR, and mass spectrometry analysis of fraction M8a (the one with the highest antimutagenic activity), suggest that the compound responsible for its antimutagenicity is an apocarotenoid.

  20. Antimutagenic Compounds of White Shrimp (Litopenaeus vannamei): Isolation and Structural Elucidation

    PubMed Central

    López-Saiz, Carmen-María; Hernández, Javier; Cinco-Moroyoqui, Francisco-Javier; Velázquez, Carlos; Ocaño-Higuera, Víctor-Manuel; Plascencia-Jatomea, Maribel; Robles-Sánchez, Maribel; Machi-Lara, Lorena; Burgos-Hernández, Armando

    2016-01-01

    According to the World Health Organization, cancer is the main cause of mortality worldwide; thus, the search of chemopreventive compounds to prevent the disease has become a priority. White shrimp (Litopenaeus vannamei) has been reported as a source of compounds with chemopreventive activities. In this study, shrimp lipids were extracted and then fractionated in order to isolate those compounds responsible for the antimutagenic activity. The antimutagenic activity was assessed by the inhibition of the mutagenic effect of aflatoxin B1 on TA98 and TA100 Salmonella tester strains using the Ames test. Methanolic fraction was responsible for the highest antimutagenic activity (95.6 and 95.9% for TA98 and TA100, resp.) and was further separated into fifteen different subfractions (M1–M15). Fraction M8 exerted the highest inhibition of AFB1 mutation (96.5 and 101.6% for TA98 and TA100, resp.) and, after further fractionation, four subfractions M8a, M8b, M8c, and M8d were obtained. Data from 1H and 13C NMR, and mass spectrometry analysis of fraction M8a (the one with the highest antimutagenic activity), suggest that the compound responsible for its antimutagenicity is an apocarotenoid. PMID:27006678

  1. Hydrophobic substituents increase the potency of salacinol, a potent α-glucosidase inhibitor from Ayurvedic traditional medicine 'Salacia'.

    PubMed

    Tanabe, Genzoh; Xie, Weijia; Balakishan, Gorre; Amer, Mumen F A; Tsutsui, Nozomi; Takemura, Haruka; Nakamura, Shinya; Akaki, Junji; Ninomiya, Kiyofumi; Morikawa, Toshio; Nakanishi, Isao; Muraoka, Osamu

    2016-08-15

    Using an in silico method, seven analogs bearing hydrophobic substituents (8a: Me, 8b: Et, 8c: n-Pent, 8d: n-Hept, 8e: n-Tridec, 8f: isoBu and 8g: neoPent) at the 3'-O-position in salacinol (1), a highly potent natural α-glucosidase inhibitor from Ayurvedic traditional medicine 'Salacia', were designed and synthesized. In order to verify the computational SAR assessments, their α-glucosidase inhibitory activities were evaluated in vitro. All analogs (8a-8g) exhibited an equal or considerably higher level of inhibitory activity against rat small intestinal α-glucosidases compared with the original sulfonate (1), and were as potent as or higher in potency than the clinically used anti-diabetics, voglibose, acarbose or miglitol. Their activities against human maltase exhibited good relationships to the results obtained with enzymes of rat origin. Among the designed compounds, the one with a 3'-O-neopentyl moiety (8g) was most potent, with an approximately ten fold increase in activity against human maltase compared to 1.

  2. Synthesis, biological evaluation and molecular modeling of 4,6-diarylpyrimidines and diarylbenzenes as novel non-nucleosides HIV-1 reverse transcriptase inhibitors.

    PubMed

    Ribone, Sergio R; Leen, Volker; Madrid, Marcela; Dehaen, Wim; Daelemans, Dirk; Pannecouque, Christophe; Briñón, Margarita C

    2012-12-01

    A series of novel 4,6-diarylpyrimidines (4,6-DAPY) and diarylbenzenes (DABE) compounds were synthesized and evaluated as inhibitors of human immunodeficiency virus type-1 (HIV-1). Among them, the most potent HIV-1 inhibitors were 8b, 8d, 14b and 18 (EC(50) = 0.049, 0.381, 0.599 and 0.398 μM, respectively), with HIV-1 inhibitory activity improved or similar to nevirapine (NVP, EC(50) = 0.097 μM) and delavirdine (DEV, EC(50) = 0.55 μM). The other compounds displayed moderate activity (8c, EC(50) = 5.25 μM) or were inactive (8a and 14a) against HIV-1 replication. Molecular modeling studies were performed with the synthesized compounds in complex with the wild-type reverse transcriptase (RT). A correlation was found between the anti-HIV activity and the electrostatic energy of interaction with Lys101 residue. These findings enrich the SAR of these Non-Nucleoside Reverse Transcriptase Inhibitors (NNRTIs) families.

  3. Implementation and Optimization of the Advanced Encryption Standard Algorithm on all 8-Bit Field Programmable Gate Array Hardware Platform

    DTIC Science & Technology

    2007-03-01

    82 84 86 88 8A 8C 8E 90 92 94 96 98 9A 9C 9E 5 A0 A2 A4 A6 A8 AA AC AE B0 B2 B4 B6 B8 BA BC BE 6 C0 C2 C4 C6 C8 CA CC CE D0 D2 D4 D6 D8 DA DC DE 7 E0...8E 90 92 94 96 98 9A 9C 9E 5 A0 A2 A4 A6 A8 AA AC AE B0 B2 B4 B6 B8 BA BC BE 6 C0 C2 C4 C6 C8 CA CC CE D0 D2 D4 D6 D8 DA DC DE 7 E0 E2 E4 E6 E8 EA EC...55 4B 49 4F 4D 43 41 47 45 B 7B 79 7F 7D 73 71 77 75 6B 69 6F 6D 63 61 67 65 C 9B 99 9F 9D 93 91 97 95 8B 89 8F 8D 83 81

  4. Pesticide Applicator Training Manual, Category 8B: Mosquito Control for New Jersey. A Training Program for the Certification of Commercial Pesticide Applicators, and Study Questions.

    ERIC Educational Resources Information Center

    Schulze, Terry L., Ed.; Kriner, Ray R., Ed.

    This training manual provides information needed to meet the mimimum EPA standards for certification as a commercial applicator of pesticides in the mosquito control category. The text discusses the aspects of mosquito biology and control by biological, mechanical, and integrated measures. A study guide with sample and study questions is included.…

  5. AV-8B Map System II: Moving-Map Composer (MMC) Version 3.5, Software User’s Manual (Third Edition)

    DTIC Science & Technology

    2007-11-02

    Table 1). Table 1 - ID Numbers, System Names, and Locations of Map-Il Stations M System Name Location 0 VOODOO NRL, Stennis, MS (old NRL computer...define a polygon; 2) entering a series of lat/lon points to define a polygon; 3) using the mouse to draw a "rubber band " box around a coverage; and 4...defines a rectangular area of coverage using a "rubber- band " box. Click on this button, then click on the upper-left corner of the area to be defined

  6. A Characterization of V-391/H46-8B, A Toughened BMI Prepreg System Produced by U.S. Polymeric

    DTIC Science & Technology

    1990-02-01

    includes plots of 4-point flex data so that the affects of hot/wet conditions can be visualized. Mechanical test results were typical of modified BMI ... composites . An interesting feature however, is that an increase in strength was noted between the RT dry and RT wet tests. The results show that the flex

  7. A 120nW 8b Sub-ranging SAR ADC with Signal-Dependent Charge Recycling for Biomedical Applications.

    PubMed

    Jeong, Seokhyeon; Jung, Wanyeong; Jeon, Dongsuk; Berenfeld, Omer; Oral, Hakan; Kruger, Grant; Blaauw, David; Sylvester, Dennis

    2015-06-01

    We present an 8-bit sub-ranging SAR ADC designed for bursty signals having long time periods with small code spread. A modified capacitive-DAC (CDAC) saves previous sample's MSB voltage and reuses it throughout subsequent conversions. This prevents unnecessary switching of large MSB capacitors as well as conversion cycles, reducing energy consumed in the comparator and digital logic and yielding total energy savings of 2.6×. In 0.18μm CMOS, the ADC consumes 120nW at 0.6V and 100kS/s with 46.9dB SNDR.

  8. Synthesis of Key Points from the OSEP Early Childhood Transition FAQ (SPP/APR Indicators C-8, B-11 and B-12)

    ERIC Educational Resources Information Center

    Diefendorf, M.; Henson, J.; Lucas, A.; Whaley, K.

    2010-01-01

    This document is a synthesis of the key points provided in the Office of Special Education Programs (OSEP) Early Childhood Transition FAQs: SPP/APR indicators C-8 and B-12 released on December 1, 2009. It was developed to assist states with the implementation of effective transition policies and practices. The following is included: (1) Transition…

  9. 75 FR 27972 - Airworthiness Directives; Pratt & Whitney JT8D-9, -9A, -11, -15, -17, and -17R Turbofan Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-19

    .... This proposed AD would require overhauling fan blade leading edges at the first shop visit after 4,000 cycles-in-service (CIS) since the last total fan blade overhaul was performed. This proposed AD results from reports of failed fan blades. We are proposing this AD to prevent high-cycle fatigue cracking...

  10. Cloning and characterization of Rep-8 (D8S2298E) in the human chromosome 8p11.2-p12

    SciTech Connect

    Yamabe, Yukako; Ichikawa, Koji; Sugawara, Kahori

    1997-01-15

    A novel human gene referred to as the Rep-8 gene (D8S2298E) was cloned by a combination of exon trapping, thermal asymmetric interlaced-PCR, and screening of a cDNA library. It is located in human chromosome 8p.11.2-p12. The gene consists of eight exons and spans about 20 kb between the glutathione S-reductase and the protein phosphatase 2A beta subunit genes. The full-length Rep-8 gene contains 1483 nucleotides and codes for a protein of 270 amino acids. Southern blot experiments showed that the Rep-8 gene exists as a single copy per haploid. With a zoo blot analysis, human Rep-8 DNA hybridized strongly with the monkey DNA, but only weakly with the DNAs of species other than Homo sapiens. Northern blot analysis showed that it is expressed abundantly in the testis and ovary, suggesting that the Rep-8 gene product may play a role in reproduction. 16 refs., 5 figs., 1 tab.

  11. 75 FR 62319 - Airworthiness Directives; Pratt & Whitney JT8D-9, -9A, -11, -15, -17, and -17R Turbofan Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-08

    ... root, which could result in uncontained failures of first stage fan blades and damage to the airplane.... The extension would allow time to determine the root cause of a fan blade failure on a Delta DC-9... rulemaking process. The root cause investigation can continue after the AD is issued. We did not change...

  12. Wind tunnel test of model target thrust reversers for the Pratt and Whitney aircraft JT8D-100 series engines installed on a 727-200 airplane

    NASA Technical Reports Server (NTRS)

    Hambly, D.

    1974-01-01

    The results of a low speed wind tunnel test of 0.046 scale model target thrust reversers installed on a 727-200 model airplane are presented. The full airplane model was mounted on a force balance, except for the nacelles and thrust reversers, which were independently mounted and isolated from it. The installation had the capability of simulating the inlet airflows and of supplying the correct proportions of primary and secondary air to the nozzles. The objectives of the test were to assess the compatibility of the thrust reversers target door design with the engine and airplane. The following measurements were made: hot gas ingestion at the nacelle inlets; model lift, drag, and pitching moment; hot gas impingement on the airplane structure; and qualitative assessment of the rudder effectiveness. The major parameters controlling hot gas ingestion were found to be thrust reverser orientation, engine power setting, and the lip height of the bottom thrust reverser doors on the side nacelles. The thrust reversers tended to increase the model lift, decrease the drag, and decrease the pitching moment.

  13. Tracing WR wind structures by using the orbiting companion in the 29d WC8d + O8-9IV binary CV Ser

    NASA Astrophysics Data System (ADS)

    David-Uraz, Alexandre; Moffat, Anthony F. J.; Chené, André Nicolas; Lange, Nicholas

    2011-01-01

    We have obtained continuous, high-precision, broadband visible photometry from the MOST satellite of CV Ser over more than a full orbit in order to link the small-scale light-curve variations to extinction due to wind structures in the WR component, thus permitting us to trace these structures. The light-curve presented unexpected characteristics, in particular eclipses with a varying depth. Parallel optical spectroscopy from the Mont Megantic Observatory and Dominion Astrophysical Observatory was obtained to refine the orbital and wind-collision parameters, as well as to reveal line emission from clumps.

  14. Using the orbiting companion to trace WR wind structures in the 29d WC8d + O8-9IV binary CV Ser

    NASA Astrophysics Data System (ADS)

    David-Uraz, Alexandre; Moffat, Anthony F. J.

    2011-07-01

    We have used continuous, high-precision, broadband visible photometry from the MOST satellite to trace wind structures in the WR component of CV Ser over more than a full orbit. Most of the small-scale light-curve variations are likely due to extinction by clumps along the line of sight to the O companion as it orbits and shines through varying columns of the WR wind. Parallel optical spectroscopy from the Mont Megantic Observatory is used to refine the orbital and wind-collision parameters, as well as to reveal line emission from clumps.

  15. Ground Wave Emergency Network. Final Operational Capability. Environmental Assessment for South Central Montana Relay Node, Site No. RN8C925MT

    DTIC Science & Technology

    1993-02-11

    Candela A unit of measure of the intensity of light equal to the brightness of one candle Carboniferous Period of geologic time from 286 to 360 million...significant impacts (Watson, CGS-4 and Meigs, CGS-9). During the 6-week construction period , the project would cause temporary and insignificant air quality...only 2 hours every week for testing purposes and for additional periods as required during power outages. Thus, the generator will operate for a

  16. Technical Reference Manual: NATO Planning Guide for the Estimation of Chemical, Biological, Radiological, and Nuclear (CBRN), Casualties, Allied Medical Publication-8(C)

    DTIC Science & Technology

    2010-08-01

    Factors a. Radiation Shielding Initial ionizing radiation exposure occurs as external irradiation with gamma and neutron radiation from the nuclear...15 year old boy who had been “frequently bitten” by insects —this case was not used for two reasons: the boy was a teen and not within the age

  17. Ground Wave Emergency Network Final Operational Capability: Environmental Assessment for Northwestern Nebraska Relay Node, Site Number RN 8C930NE

    DTIC Science & Technology

    1993-02-19

    migrans 2 Swift fox Vulpes velox Canidae 2 Yellow mud turtle Kinosternon flavescens Kinosternidac (northern population) flavescens C-7 UXEýUV - United...ign niJgje Laridae 2 Harlequin duck Histrionicum Anaatidae hiutrionigue 2 Swift fox y•GI velax Canidae 2 Fringed-tailed myotis •1i tvg

  18. Longitudinal aerodynamic characteristics of a wing-winglet model designed at M = 0.8, C sub L = 0.4 using linear aerodynamic theory

    NASA Technical Reports Server (NTRS)

    Kuhlman, J. M.

    1983-01-01

    Wind tunnel test results have been presented herein for a subsonic transport type wing fitted with winglets. Wind planform was chosen to be representative of wings used on current jet transport aircraft, while wing and winglet camber surfaces were designed using two different linear aerodynamic design methods. The purpose of the wind tunnel investigation was to determine the effectiveness of these linear aerodynamic design computer codes in designing a non-planar transport configuration which would cruise efficiently. The design lift coefficient was chosen to be 0.4, at a design Mach number of 0.8. Force and limited pressure data were obtained for the basic wing, and for the wing fitted with the two different winglet designs, at Mach numbers of 0.60, 0.70, 0.75 and 0.80 over an angle of attack range of -2 to +6 degrees, at zero sideslip. The data have been presented without analysis to expedite publication.

  19. 75 FR 50891 - N-alkyl (C8-C18) Primary Amines and Acetate Salts; Exemption from the Requirement of a Tolerance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... CFR 180.930 at a maximum concentration in formulated end-use products of 10% by weight in herbicide... concentration in formulated end-use products not to exceed 10% by weight in herbicide products, 4% by weight in..., herbicides, and fungicides. A complete description of the general approach taken to assess inert...

  20. Ground Wave Emergency Network Final Operational Capability: Environmental Assessment for Central Utah Relay Node, Site Number RN 8C920UT

    DTIC Science & Technology

    1993-04-16

    March 29, 1990. Burt, W.H., and R. P. Grossenheider, 1976. A Field Guide to the Mammals: North America North of Mexico, 3rd Edition. Houghton Mifflin...The Birder’s Handbook, a Field Guide to the Natural History of North American Birds. Fireside Books, Simon and Schuster, Inc., New York, New York

  1. Testing the Itinerancy of Spin Dynamics in Superconducting Bi2Sr2CaCu2O8C

    SciTech Connect

    Xu, G.; Gu, G.D.; Hücker, M.; Fauqué, B.; Perring, T.G.; Regnault, L.P.; Tranquada, J.M.

    2009-09-01

    Much of what we know about the electronic states of high-temperature superconductors is due to photoemission and scanning tunnelling spectroscopy studies of the compound Bi{sub 2}Sr{sub 2}CaCu{sub 2}O{sub 8+{delta}}. The demonstration of well-defined quasiparticles in the superconducting state has encouraged many theorists to apply the conventional theory of metals, Fermi-liquid theory, to the cuprates. In particular, the spin excitations observed by neutron scattering at energies below twice the superconducting gap energy are commonly believed to correspond to an excitonic state involving itinerant electrons. Here, we present the first measurements of the magnetic spectral weight of optimally doped Bi{sub 2}Sr{sub 2}CaCu{sub 2}O{sub 8+{delta}} in absolute units. The lack of temperature dependence of the local spin susceptibility across the superconducting transition temperature, T{sub c}, is incompatible with the itinerant calculations. Alternatively, the magnetic excitations could be due to local moments, as the magnetic spectrum is similar to that in La{sub 1.875}Ba{sub 0.125}CuO{sub 4}, where quasiparticles and local moments coexist.

  2. Materials Data on H8C2SeS2(BrN2)2 (SG:14) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-04-22

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  3. W-026, acceptance test report gamma energy assay (GEA) system A(submittal {number_sign}39.8) C3

    SciTech Connect

    Watson, T. L.

    1997-01-28

    This test procedure is designed to test all of the functions of the GEA system at the WRAP facility at Hanford, Washington. The procedure is designed to be executed in the sequence given, starting with: * verification of components and labeling * verification or entry of all of the setup parameters * performing the calibrations (energy, efficiency, transmission, and reference peak) * performing the measurements in local mode * performing the measurements in remote mode * confirming the message formats * utility operations (LN2 fill, archiving, password maintenance) The system is designed to be operated in a remote mode in which all commands are received over a network, and the results are returned over the same network. A manual mode is provided for the setup and calibration operations, and for maintenance functions. The system is designed to start up in remote mode. The user can go to local mode when at the keyboard by selecting Exit on the screen shown during remote mode. A user logon screen, requiring 2 username and password, limits access to local mode.

  4. 40 CFR Table 10 to Subpart Uuuu of... - Applicability of General Provisions to Subpart UUUU

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... calibration drifts No. Replaced with language in § 63.5560. § 63.8(c)(4)(i) and (ii) Continuous Monitoring...-control periods No. Replaced with language in § 63.5545. § 63.8(c)(7) and (8) CMS Requirements Out-of-control periods, including reporting No. Replaced with language in § 63.5580(c)(6). § 63.8(d) CMS...

  5. Balancing the Direct and Indirect Approaches: Implications for Ending the Violence in Southern Thailand

    DTIC Science & Technology

    2009-12-01

    6 3. Insurgency Groups in Thailand.............................................................. 7 a. Barisan Nasional Pemberbasan Pattani...BNPP)........................ 8 b. Barisan Revolusi Nasional (BRN) ............................................... 8 c. Patani United Liberation...BLANK xiii LIST OF ACRONYMS AND ABBREVIATIONS AFP Philippines Armed Forces ASG Abu Sayyaf Group BNPP Barisan Nasional

  6. 12 CFR Appendix A to Part 363 - Guidelines and Interpretations

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., including the prevention or detection of management override or compromise of the internal control system.... Management's Reports on Internal Control over Financial Reporting under Part 363 and Section 404 of SOX 8B. Internal Control Reports and Part 363 Annual Reports for Acquired Businesses 8C. Management's Disclosure...

  7. Reproductive and Hormonal Risk Factors for Breast Cancer in Blind Women

    DTIC Science & Technology

    2005-06-01

    Retinitis pigmentosa or other retinal dystrophies _ Retinal ...detachment Glaucoma _Optic nerve disease _Other eye disease 8b. Left Eye: "Dry" Macular Degeneration "Wet" Macular Degeneration __. Retinitis pigmentosa or...other retinal dystrophies _ Retinal detachment RetGlaucoma Optic nerve disease Other eye disease 8c. Have either of your eyes been enucleated

  8. 12 CFR Appendix A to Part 363 - Guidelines and Interpretations

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Branches of Foreign Banks 7A. Compliance with Designated Laws and Regulations 8. Management Report 8A. Management's Reports on Internal Control over Financial Reporting under Part 363 and Section 404 of SOX 8B. Internal Control Reports and Part 363 Annual Reports for Acquired Businesses 8C. Management's Disclosure...

  9. 15 CFR 764.8 - Voluntary self-disclosures for boycott violations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...-disclosure as described in paragraph (c)(2) of this section before it commences an investigation or inquiry... the disclosure subsequently completes the narrative account required by § 764.8(c)(3) of this part... of § 764.8(b)(3) of this part. (3) Narrative account. After the initial notification, a...

  10. 76 FR 56304 - Hazardous Materials: Minor Editorial Corrections and Clarifications

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-13

    ....115(k)(6). Some of the information for the entry ``Argon, compressed UN1006'' in the HMT, while... ``Argon, compressed UN1006'' by correcting the information reported in columns 5, 6, 7, 8a, 8b, 8c, 9a, 9b... exceeding 1 L capacity). * * * * * * * Argon, compressed. 2.2 UN1006 2.2 306, 307...... 302 314,...

  11. Fine needle aspiration biopsy of suspected metastatic cancers to the posterior uvea.

    PubMed Central

    Augsburger, J J

    1988-01-01

    This thesis presents the author's experience with diagnostic intraocular fine needle aspiration biopsy in 18 patients with a suspected metastatic choroidal or ciliary body tumor. The author has reviewed the literature on biopsy of intraocular tumors and has specified what he believes to be valid indications for diagnostic biopsy of posterior uveal tumors. He has evaluated the accuracy, limitations, and complications of diagnostic fine needle aspiration biopsy in this series and others, and he has suggested methods for improving the recovery of sufficient cells for cytologic diagnosis and lessening the risks of tumor cell seeding during the biopsy. The author has concluded that fine needle aspiration biopsy appears to be a relatively safe, generally reliable means of establishing the pathologic diagnosis of a choroidal or ciliary body tumor in highly selected patients suspected of having metastatic cancer. In spite of its apparent safety and reliability, however, the author has cautioned against the routine use of fine needle aspiration biopsy in patients with posterior uveal tumors since its long-term safety has not been established. The author has suggested that diagnostic fine needle aspiration biopsy of posterior uveal tumors be performed only in medical centers where there can be input from and cooperation among ophthalmologists, ophthalmic pathologists, and cytopathologists who are experienced in the diagnosis of intraocular malignancies. Images FIGURE 3 A FIGURE 3 B FIGURE 4 A FIGURE 4 B FIGURE 4 C FIGURE 4 D FIGURE 5 A FIGURE 5 B FIGURE 5 C FIGURE 5 D FIGURE 6 A FIGURE 6 B FIGURE 7 A FIGURE 7 B FIGURE 7 C FIGURE 7 D FIGURE 8 A FIGURE 8 B FIGURE 8 C FIGURE 8 D FIGURE 9 A FIGURE 9 B FIGURE 9 C FIGURE 9 D FIGURE 10 A FIGURE 10 B FIGURE 10 C FIGURE 11 PMID:2979028

  12. Analysis of Early Separation Incentive Options to Shape the Naval Force of the Future

    DTIC Science & Technology

    2004-12-01

    8 C. TEMPORARY EARLY RETIREMENT ACT (TERA)..............................8 D. CAREER STATUS BONUS (CSB...Incentive (VSI), the Selective Separation Benefit (SSB), the Temporary Early Retirement Act (TERA), the Career Status Bonus (CSB), involuntary separation...maintained temporarily. C. TEMPORARY EARLY RETIREMENT ACT (TERA) TERA is a program which allows personnel to retire before they reach 20 years of

  13. 40 CFR Table 6 to Subpart Cccc of... - Applicability of General Provisions to Subpart CCCC

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Requirements 1. For § 63.6(e) and (f), requirements for startup, shutdown, and malfunctions apply only to.... 3. For § 63.8(c)(1), requirements for startup, shutdown, and malfunctions apply only to malfunctions, and no report pursuant to § 63.10(d)(5)(i) is required. 4. For § 63.8(d), requirements for...

  14. 40 CFR Table 3 to Subpart Bbbbbb... - Applicability of General Provisions

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...); COMS are properly maintained and operated according to § 63.8(c) and data quality as § 63.8(d) No. § 63... (COMS) Monitoring Data from Performance Test Must submit COMS data with other performance test data No. § 63.6(h)(7)(ii) Using COMS Instead of EPA Method 9 Can submit COMS data instead of EPA Method...

  15. Multigrid Methods in Network Optimization: Overview and Appraisal

    DTIC Science & Technology

    1994-03-01

    Tucker ( KKT ) optimality conditions . These are necessary and sufficient for linear programming problems. The case of equality constraints is specifically...8 C. KARUSH-KUHN-TUCKER ( KKT ) OPTIMALITY CONDITIONS ....... 8 D. OPTIMALITY CONDITIONS IN NETWORKS...considered. A key result in establishing the KKT conditions is the lemma presented in section B. Finally, in section D we specialize the optimality

  16. Revisiting the chemistry of the actinocenes [(η8-C8H8)2An] (An = U, Th) with neutral Lewis bases. Access to the bent sandwich complexes [(η8-C8H8)2An(L)] with thorium (L = py, 4,4'-bipy, tBuNC, R4phen).

    PubMed

    Berthet, Jean-Claude; Thuéry, Pierre; Garin, Nicolas; Dognon, Jean-Pierre; Cantat, Thibault; Ephritikhine, Michel

    2013-07-10

    In stark contrast to uranocene, (Cot)2Th reacts with neutral mono- or bidentate Lewis bases to give the bent sandwich complexes (Cot)2Th(L) (L = py, 4,4'-bipy, tBuNC, phen, Me4phen). DFT calculations in the gas phase show that, for both U and Th, formation of the bent compound (Cot)2An(L) should be facile, the linear and bent forms being close in energy.

  17. PROCEEDINGS: 1991 INTERNATIONAL CONFERENCE ON MUNICIPAL WASTE COMBUSTION - VOLUME 2. SESSIONS 1B, 2B, 3B, 4B, 7A, 7B, 8A, 8B, AND 9B

    EPA Science Inventory

    The three-volumes document 82 presentations by authors from 15 countries at the Second International Conference on Municipal Waste Combustion (MWC) in Tampa, Florida, April 16-19, 1991. The Conference fostered the exchange of current information on research concerning MWC, ash di...

  18. The Attorney General's Asbestos Liability Report to the Congress. Pursuant to Section 8(b) of the Asbestos School Hazard Detection and Control Act of 1980. Committee Print, Ninety-Seventh Congress, First Session.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and Labor.

    The Attorney General was directed by Congress to prepare a report on whether the United States could recover, from any persons determined liable, the amounts expended to detect, contain, or remove hazardous asbestos products from schools. The general background portion of this report contains the results of the factual research and investigation.…

  19. The Synchrotron Radiation Beamline 8-b at VEPP-4 Collider for SAXS, WAXS and Micro Tomography Investigation of Fast Processes at Extreme Condition of High Temperature and Pressure with Nanosecond Time Resolution

    NASA Astrophysics Data System (ADS)

    Tolochko, B. P.; Kosov, A. V.; Evdokov, O. V.; Zhogin, I. L.; Ten, K. A.; Pruuel, E. R.; Shekchtman, L. I.; Aulchenko, V. M.; Zhulanov, V. V.; Piminov, P. F.; Nazmov, V. P.; Zolotarev, K. V.; Kulipanov, G. N.

    The main purpose of the beamline design - realization of experiments using explosive charges up to 200 grams of TNT equivalent. To achieve this goal is necessary to use a of hard range of photons in the region of 30-60 keV. Additional requirement - the development of a powerful explosion chamber, and very fast one coordinate detector DIMEX. The fist detonation experiments was made with explosive 40 mm diameter. Test WAXS experiments was made and time resolution of 73 ps was received. SAXS/WAXS experiments was made at accelerators complex VEPP-3/VEPP-4.

  20. Exchange Coupling in an Isostructural Series of Face-Sharing Bioctahedral Complexes [LM(II)(&mgr;-X)(3)M(II)L]BPh(4) (M = Mn, Fe, Co, Ni, Zn; X = Cl, Br; L = 1,4,7-Trimethyl-1,4,7-triazacyclononane).

    PubMed

    Bossek, Ursula; Nühlen, Daniela; Bill, Eckhard; Glaser, Thorsten; Krebs, Carsten; Weyhermüller, Thomas; Wieghardt, Karl; Lengen, Marek; Trautwein, A. X.

    1997-06-18

    The reaction of the divalent metal halides ZnCl(2), ZnBr(2), MnCl(2).4CH(3)CN, MnBr(2), FeCl(2).4CH(3)CN, CoCl(2).4CH(3)CN, CoBr(2), NiCl(2).6H(2)O, and NiBr(2), respectively, with the macrocycle 1,4,7-trimethyl-1,4,7-triazacyclononane (L) (1:1) in anhydrous acetonitrile, acetone, chloroform, or ethanol affords upon additon of NaBPh(4) the isomorphous series of complexes [LM(II)(&mgr;-X)(3)M(II)L]BPh(4): 1, M = Zn, X = Cl; 2, Zn, Br; 3, Mn, Cl; 4, Mn, Br; 5, Fe, Cl; 6, Co, Cl; 7, Co, Br; 8, Ni, Cl; 9, Ni, Br. Six of these complexes have been structurally characterized by single-crystal X-ray crystallography; they crystallize in the triclinic space group P&onemacr; (No. 2) with Z = 4. Crystal data are as follows 1, a = 16.654(1), b = 17.042(1), c = 17.684(1) Å, alpha = 97.30(1), beta = 93.58(1), gamma = 117.46(1) degrees; 3, a = 16.632(8), b = 17.012(8), c = 17.855(5) Å, alpha = 97.16(3), beta = 93.37(3), gamma = 117.24(3) degrees; 5, a = 16.658(3), b = 17.064(3), c = 17.741(4) Å, alpha = 97.32(3), beta = 93.47(3), gamma = 117.36(3) degrees; 6, a = 16.640(3), b = 17.040(3), c = 17.686(4) Å, alpha = 97.39(3), beta = 93.58(3), gamma = 117.39(3) degrees; 8, a = 16.608(3), b = 16.995(3), c = 17.555(3) Å, alpha = 97.36(1), beta = 93.52(1), gamma = 117.52(1) degrees; 9, a = 16.680(3), b = 17.016(2), c = 17.715(3)Å, alpha = 96.99(1), beta = 93.70(1), gamma = 117.42(1) degrees. All complexes consist of a dinuclear, face-sharing bioctahedral monocation with three &mgr;(2)-Cl or &mgr;(2)-Br bridging ligands and two LM fragments and well-separated tetraphenylborate anions (1:1). The cations cocrystallize in two different forms: an enantiomeric form with (lambdalambdalambda) (or (deltadeltadelta)) conformation at both LM fragments and a meso form with an (lambdalambdalambda) conformation at one LM fragment and (deltadeltadelta) at the other (ratio 1:1). From temperature-dependent magnetic susceptibility measurements (2-293 K) it was established that the spins of the

  1. Program on ground test of modified quiet, clean, JT3D and JT8D turbofan engines in their respective nacelles. [modification of Boeing 707, 727, and 737 aircraft for aircraft noise reduction

    NASA Technical Reports Server (NTRS)

    1973-01-01

    A program to reduce the community noise levels of commercial jet aircraft is summarized. The program objective is the development of three acoustically treated nacelle configurations for the 707, 727, and 737 series aircraft to provide maximum noise reduction with minimum performance loss, modification requirements, and economic impact. The preliminary design, model testing, data analyses, and economic studies of proposed nacelle configurations are discussed.

  2. PACAF (Pacific Air Forces) Red Shirt/Farm Club: a New Look.

    DTIC Science & Technology

    1988-04-01

    PERFORMING ORGANIZATION 6b. OFFICE SYMBOL 7a. NAME OF MONITORING ORGANIZATION (It applicable) ACSC/EDC I 6c. ADDRESS ( City , State, and ZlPCode) 7b...ADDRESS( City , State, and ZIP Code) Miaxwell AFB AL 36112-5542 Ba. NAME OF FUNDING/SPONSORING 8b. OFFICE SYMBOL 9. PROCUREMENT INSTRUMENT IDENTIFICATION...NUMBER ORGANIZATION (If applicable) 8c. ADDRESS ( City , State, and ZIP Code) 10. SOURCE OF FUNDING NUMBERS PROGRAM PROJECT TASK WORK UNITELEMENT NO. NO

  3. Unsteady Three-Dimensional Euler Equations Solutions on Dynamic Blocked Grids.

    DTIC Science & Technology

    1986-10-01

    OF REPORT Approved for public release; distribution 2b DECLASSIFICATION/ DOWNGRADING SCHEDULE unlimited. 4. PERFORMING ORGANIZATION REPORT NUMBER (S) 5...MONITORING ORGANIZATION REPORT NUMBER (S) r .- ; AFATL-TR--36-74 ’ ~’ 6a NAME OF PERFORMING ORGANIZATION 6b OFFICE SYMBOL 7a NAM[OF MONITORING...8b OFFICE SYMBOL 9 PROCUREMENT INSTRUMENT IDENTIFICATION NUMBER ORGANIZATION (If a plicable) Air Force Armament Laboratory ATL/FXA 8c. ADDRESS (City

  4. Synthesis and structure-activity studies of benzyl ester meperidine and normeperidine derivatives as selective serotonin transporter ligands.

    PubMed

    Gu, Xiaobo; Izenwasser, Sari; Wade, Dean; Housman, Amy; Gulasey, Gerard; Rhoden, Jill B; Savoie, Christopher D; Mobley, David L; Lomenzo, Stacey A; Trudell, Mark L

    2010-12-01

    A series of benzyl esters of meperidine and normeperidine were synthesized and evaluated for binding affinity at serotonin, dopamine and norepinephrine transporters. The 4-methoxybenzyl ester 8b and 4-nitrobenzyl ester 8c in the meperidine series and 4-methoxybenzyl ester 14a in the normeperidine series exhibited low nanomolar binding affinities at the SERT (K(i) values <2nM) and high SERT selectivity (DAT/SERT >1500 and NET/SERT >1500).

  5. Study of Energy Levels and Decay Mechanisms for Singlet Rydberg States of Molecular Nitrogen.

    DTIC Science & Technology

    1987-09-15

    C.4 Harvard University Holyoke Center 1350 Massachusetts Avenue Cambridge, MA 02138 C1 Final Report 1 October 1983 - 1 August 1987 15 September 1987... Holyoke Center 1350 Massachusetts Avenue Hanscom AFB, MA 01731-5000 Cambridge, MA 02138 8a. NAME OF FUNDING/SPONSORING 8b OFFICE SYMBOL 9. PROCUREMENT...INSTRUMENT IDENTIFICATION NUMBER ORGANIZATION (if applicable) F19628-84-K-0015 8c. ADDRESS (City, State, and ZIP Code) 10. SOURCE OF FUNDING NUMBERS

  6. Design and Construction of Mat Foundations

    DTIC Science & Technology

    1989-11-01

    Waterways Experiment Station, Corps of Engineers 3909 Halls Ferry Road, Vicksburg, Mississippi 39180-6199 /WA) DTIC ELECTE ,. . ~S DEC291989 November...1989 Appre Fr PFinal Report ’II Prepared for DEPARTMENT OF THE ARMY US Army Corps of Engineers Washington, DC 20314-1000 -LABORATORY Under RDT&E Work...OF FUNDINGSPONSORING 8b OFFICE SYMBOL 9 PROCUREMENT INSTRUMENT IDENTIFICATION NUMBER ORGANIZATION US Army Corps of (If applicable) Engineers I 8c

  7. Evaluation of Membrane Systems for Washing/Deglycerolizing Packed Red Blood Cells

    DTIC Science & Technology

    1987-03-20

    CLASSIFICATION Of THIS PAGE REPORT DOCUMENTATION PAGE Form Approved OM8 No 0704 0188 la REPORT SECURITY CLASSIFICATION Unclassified lb RESTRICTIVE...Bend Research, Inc. 6b OFFICE SYMBOL (if •pptktbh) 7a. NAME OF MONITORING ORGANIZATION •c AOOfttSS (City. State. end ZIP Code) 64550 Research Road...8b OFFICE SYMBOL (If ipplktble) 9 PROCUREMENT INSTRUMENT IDENTIFICATION NUMBER DAMD17-86-C-6142 8c AOORESSfOfy. Stttr ind ZIP Cod*) Fort

  8. Bomber Force 2000: Operational Concepts for Long-Range Combat Aircraft

    DTIC Science & Technology

    1994-02-08

    ORGAIhiZATtON (if applicable ) OPERATIONS DEPARTMENT C 6c. ADDRESS (City, State, and ZIP Code) 7b ADDRESS (City. State, and ZIP Code) NAVAL WAR COLLEGE NEWPORT...RI 02841 Ba. NAME OF FUNDING iSPONSORING 8b. OFFICE SYMBOL 9 PROCUREMENT INSTRUMENT IDENTIFICATION NUMBER ORGANIZATION (If applicable ) 8c. ADDRESS...the indivisible application of air power. Strategic and tactical air operations became more associated with specific types of aircraft than with ways of

  9. Ground-Based Surveillance and Tracking System (GSTS)

    DTIC Science & Technology

    1987-08-01

    SCHEDULE UNLIMITED 4. PERFORMING ORGANiZATION REPORT NUMBER (S) 5. MONITORING ORGANIZATION REPORT NUMBER (S) 6a. NAME OF. PERFORMING ORGANIZATION 6h. OFFICE...8b. OFFICE SYMBOL 9. PROCUREMENT INSTRUMENT IDENTIFICATION NUMBER ORGANIZATION (If applicable) 8c. ADDRESS (City, State, and ZIP Code) 10. SOURCE OF...FUNDING NUMBERS PROGRAM PROJECT TASK WORK UNIT ELEMENT NO. NO. NO. ACCESSION NO. 11. TITLE (Include Security Classi6carion) Ground-Based Surveillance

  10. EMMA (Expert Missile Maintenance Aid): The Expert System for Munition Maintenance

    DTIC Science & Technology

    1988-07-01

    CONCLUDED) Electonic Systems Division 8b. OFFICE SYMBOL (CONCLUDED) ESD/XRS 8c. ADDRESS (CONCLUDED) Hanscom AFB, MA 01731-5003 19. ABSTRACT (CONCLUDED...325th Equipment Maintenance Squadron formed by the knowledge engineer . Since (EMS) at Tyndall AFB, Florida for an eval- expert system development is...development. The knowl- edge engineer and the expert verify the The argument could be made that EMMA expert system and identify potential cor- should accurately

  11. PROCEEDINGS: 1991 INTERNATIONAL CONFERENCE ON MUNICIPAL WASTE COMBUSTION - VOLUME 3. SESSIONS 1C, 2C, 3C 4C, 6C, 7C, 8C, 9A, AND 10A/C

    EPA Science Inventory

    The three-volumes document 82 presentations by authors from 15 countries at the Second International Conference on Municipal Waste Combustion (MWC) in Tampa, Florida, April 16-19, 1991. The Conference fostered the exchange of current information on research concerning MWC, ash di...

  12. Addenda to Allied Medical Publication 8, NATO Planning Guide for the Estimation of Chemical, Biological, Radiological, and Nuclear (CBRN) Casualties (AMedP-8(C)) to Consider the Impact of Medical Treatment on Casualty Estimation

    DTIC Science & Technology

    2013-05-01

    Osterholm, Tara O’Toole, Gerald Parker, Trish M. Perl, Philip K. Russell , David L. Swerdlow, and Kevin Tonat. “Botulinum Toxin as a Biological Weapon...DeAngelis, Louise Cain, Barbara Wallace , and Nellie Dumas. “Laboratory-Acquired Brucellosis.” Emerging Infectious Diseases 10, no. 10 (2004): 1848–50...Amari, Alfred M. Sciuto, Richard K. Gordon, Bhupendra P. Doctor, and Madhusoodana P. Nambiar. “Post-Exposure Treatment with Nasal Atropine Methyl

  13. Abstraction and addition kinetics of C2H radicals with CH4, C2H6, C3H8, C2H4, and C3H6: CVT/SCT/ISPE and hybrid meta-DFT methods.

    PubMed

    Dash, Manas Ranjan; Rajakumar, B

    2015-02-07

    Rate coefficients for the reactions of C2H radicals with methane (k1), ethane (k2), propane (k3), ethylene (k4), and propylene (k5) were computed using canonical variational transition state theory (CVT) coupled with hybrid-meta density functional theory (DFT) over a wide range of temperatures from 150 to 5000 K. The quantum chemical tunneling effect was corrected by the small curvature tunneling (SCT) method. The dynamic calculations are performed using the variational transition state theory (VTST) with the interpolated single-point energies (ISPE) method at the CCSD(T)/cc-pVTZ//M06-2X/6-31+G(d,p) level of theory. Intrinsic reaction coordinate (IRC) calculations were performed to verify that the transition states are connected to the reactants and products. The rate coefficients obtained over the studied temperature range yield the following Arrhenius expressions (cm(3) molecule(-1) s(-1)): k1 = 4.69 × 10(-19)T(2.44) exp[331/T], k2 = 4.29 × 10(-17)T(2.11) exp[432/T], k3 = 4.81 × 10(-17)T(1.98) exp[697/T], k4 = 7.54 × 10(-21)T(2.96) exp[1942/T], and k5 = 8.04 × 10(-23)T(3.44) exp[3011/T] cm(3) molecule(-1) s(-1). Branching ratio calculation for the reactions of C2H radicals with ethylene and propylene shows that the abstraction reactions are not important at lower temperatures. However, as the temperature increases, abstraction reactions become more important.

  14. NATO Planning Guide for the Estimation of Chemical, Biological, Radiological, and Nuclear (CBRN) Casualties (AMedP-8(C)) - Parameters for Estimation of Casualties from Exposure to Specified Biological Agents. Addenda to Allied Medical Publication 8

    DTIC Science & Technology

    2011-01-01

    estimating the numbers of persons developing illness or dying from anthrax, botulism, Venezuelan equine encephalitis, plague, and smallpox. Five additional...the non-contagious biological agent explanation in Section 0106.7b, following the Venezuelan equine encephalitis (VEE) assumptions and limitations...comprehensive clinical studies of tularemia available were reported in the pre- antibiotic era before inhalation was understood to be a potential route of

  15. Refinement of the OPA1 gene locus on chromosome 3q28-q29 to a region of 2-8 cM, in one Cuban pedigree with autosomal dominant optic atrophy type Kjer

    SciTech Connect

    Lunkes, A.; Hartung, U.; Auburger, G.

    1995-10-01

    Kjer type autosomal dominant optic atrophy was reported to have a prevalence of 1:50,000 and is therefore the most common form of familial optic atrophy. Age at onset and chronic progressive course were used as subclassification criteria by Kjer, Jaeger, and Smith, stating that almost all cases manifested subacutely before 8 years of age. Typically, tritan color defects and small paracentral scotomas are found together with both variable reduction of visual acuity, of approximately 0.3/0.1, and a temporal pallor on fundoscopy. Pathologically the retinal ganglion cells are affected, resulting in a progressive degeneration of the optic nerve. 12 refs., 1 fig., 1 tab.

  16. 40 CFR Table 3 to Subpart Cccccc... - Applicability of General Provisions

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and operated according to § 63.8(c) and data quality as § 63.8(d) No. § 63.6(h)(7)(v) Determining.... § 63.6(h)(7)(i) Report Continuous Opacity Monitoring System (COMS) Monitoring Data From Performance Test Must submit COMS data with other performance test data No. § 63.6(h)(7)(ii) Using COMS Instead...

  17. 76 FR 34658 - The Internet Assigned Numbers Authority (IANA) Functions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-14

    .../attachments/Cisco.pdf ; ictQatar Comments at 1 (March 30, 2011), available at http://www.ntia.doc.gov/comments...-9FCC- 9AF8D47BC69C. \\14\\ See IAB Comments at 1; ICANN Comments at 8; ictQatar Comments at 1; UAE...-62FF-4A91-90A8-C87C8CFA9527; ISOC Comments at 3. \\23\\ See ictQatar Comments at 2. \\24\\ See...

  18. Evaluation of craniofacial surgery in the treatment of facial deformities.

    PubMed Central

    Murray, J E; Swanson, L T; Strand, R D; Hricko, G M

    1975-01-01

    . Fig. 1g. Fig. 1h. Fig. 1i. Fig. 1m. Fig. 2a. Fig. 2b., Fig. 2c., Fig. 2d. Fig. 2e. Figs. 3 a and b. Fig. 3d., Fig. 3e. Fig. 3f. Fig. 4a., Fig. 4b. Fig. 5a. Fig. 5b., Fig. 5c. Figs. 5d and e. Fig. 5f. Figs. 5g. and 5h. Fig. 6a., Fig. 6b. Fig. 6c. Fig. 7a. Fig. 7b. Figs. 7e and f. Fig. 8a. Fig. 8b. Fig. 8c. Fig. 8d., Fig. 8e. Fig. 9a. Fig. 9b. Figs. 9c. and d. Fig. 9e. Fig. 9f. Figs. 9g. and h. Figs. 11a, b, and c. Figs. 11d, e, f. PMID:169753

  19. Novel third-generation water-soluble noscapine analogs as superior microtubule-interfering agents with enhanced antiproliferative activity.

    PubMed

    Henary, Maged; Narayana, Lakshminarayana; Ahad, Shazia; Gundala, Sushma R; Mukkavilli, Rao; Sharma, Vibhuti; Owens, Eric A; Yadav, Yogesh; Nagaraju, Mulpuri; Hamelberg, Donald; Tandon, Vibha; Panda, Dulal; Aneja, Ritu

    2014-11-15

    Noscapine, an opium-derived 'kinder-gentler' microtubule-modulating drug is in Phase I/II clinical trials for cancer chemotherapy. However, its limited water solubility encumbers its development into an oral anticancer drug with clinical promise. Here we report the synthesis of 9 third-generation, water-soluble noscapine analogs with negatively charged sulfonato and positively charged quaternary ammonium groups using noscapine, 9-bromonoscapine and 9-aminonoscapine as scaffolds. The predictive free energy of solvation was found to be lower for sulfonates (6a-c; 8a-c) compared to the quaternary ammonium-substituted counterparts, explaining their higher water solubility. In addition, sulfonates showed higher charge dispersability, which may effectively shield the hydrophobicity of isoquinoline nucleus as indicated by hydrophobicity mapping methods. These in silico data underscore efficient net charge balancing, which may explain higher water solubility and thus enhanced antiproliferative efficacy and improved bioavailability. We observed that 6b, 8b and 8c strongly inhibited tubulin polymerization and demonstrated significant antiproliferative activity against four cancer cell lines compared to noscapine. Molecular simulation and docking studies of tubulin-drug complexes revealed that the brominated compound with a four-carbon chain (4b, 6b, and 8b) showed optimal binding with tubulin heterodimers. Interestingly, 6b, 8b and 8c treated PC-3 cells resulted in preponderance of mitotic cells with multipolar spindle morphology, suggesting that they stall the cell cycle. Furthermore, in vivo pharmacokinetic evaluation of 6b, 8b and 8c revealed at least 1-2-fold improvement in their bioavailability compared to noscapine. To our knowledge, this is the first report to demonstrate novel water-soluble noscapine analogs that may pave the way for future pre-clinical drug development.

  20. Chemical constituents of Plectranthus amboinicus and the synthetic analogs possessing anti-inflammatory activity.

    PubMed

    Chen, Yuan-Siao; Yu, Hui-Ming; Shie, Jiun-Jie; Cheng, Ting-Jen Rachel; Wu, Chung-Yi; Fang, Jim-Min; Wong, Chi-Huey

    2014-03-01

    This study demonstrates that compounds 1-4 from an extract of Plectranthus amboinicus inhibit the binding of AP-1 to its consensus DNA sequence. Thymoquinone (5) was further identified as a nonpolar ingredient from the hexane extract of P. amboinicus to suppress the expression of lipopolysaccharide-induced tumor necrosis factor-alpha (TNF-α). We then synthesized 2-alkylidenyl-4-cyclopentene-1,3-diones as the designed biomimetics of thymoquinone, and found that compounds 8a, 8b and 8d were more potent TNF-α inhibitors.