Science.gov

Sample records for 8b 8c 8d

  1. 2p-2p decay of {sup 8}C and isospin-allowed 2p decay of the isobaric-analog state in {sup 8}B

    SciTech Connect

    Charity, R. J.; Elson, J. M.; Manfredi, J.; Shane, R.; Sobotka, L. G.; Chajecki, Z.; Coupland, D.; Iwasaki, H.; Kilburn, M.; Lee, Jenny; Lynch, W. G.; Sanetullaev, A.; Tsang, M. B.; Winkelbauer, J.; Youngs, M.; Marley, S. T.; Shetty, D. V.; Wuosmaa, A. H.; Ghosh, T. K.

    2010-10-15

    {sup 8}C is found to decay to four protons and an {alpha} particle in two 2p emission steps. The correlations between the protons in the first step ({sup 8}C to {sup 6}Be) exhibit a significant enhancement in the region of the decay phase space where the two protons have small relative energy, a region sometimes called the diproton region. The decay of the isobaric analog of {sup 8}C in {sup 8}B is also found to decay by 2p emission. This is the first case of isospin-allowed 2p decay between isobaric analog states.

  2. The Protein Synthesis Inhibitor Blasticidin S Enters Mammalian Cells via Leucine-rich Repeat-containing Protein 8D

    PubMed Central

    Lee, Clarissa C.; Freinkman, Elizaveta; Sabatini, David M.; Ploegh, Hidde L.

    2014-01-01

    Leucine-rich repeat-containing 8 (LRRC8) proteins have been identified as putative receptors involved in lymphocyte development and adipocyte differentiation. They remain poorly characterized, and no specific function has been assigned to them. There is no consensus on how this family of proteins might function because homology searches suggest that members of the LRRC8 family act not as plasma membrane receptors, but rather as channels that mediate cell-cell signaling. Here we provide experimental evidence that supports a role for LRRC8s in the transport of small molecules. We show that LRRC8D is a mammalian protein required for the import of the antibiotic blasticidin S. We characterize localization and topology of LRRC8A and LRRC8D and demonstrate that LRRC8D interacts with LRRC8A, LRRC8B, and LRRC8C. Given the suggested involvement in solute transport, our results support a model in which LRRC8s form one or more complexes that may mediate cell-cell communication by transporting small solutes. PMID:24782309

  3. 15 CFR 8b.18 - New construction.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false New construction. 8b.18 Section 8b.18... HANDICAPPED IN FEDERALLY ASSISTED PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE Accessibility § 8b.18...

  4. 15 CFR 8b.18 - New construction.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false New construction. 8b.18 Section 8b.18... HANDICAPPED IN FEDERALLY ASSISTED PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE Accessibility § 8b.18...

  5. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  6. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  7. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  8. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  9. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  10. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  11. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  12. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  13. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  14. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  15. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  16. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  17. 15 CFR 8b.4 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Discrimination prohibited. 8b.4 Section 8b.4 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Provisions § 8b.4 Discrimination prohibited. (a) General. No qualified handicapped individual shall, on...

  18. 15 CFR 8b.11 - Discrimination prohibited.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Discrimination prohibited. 8b.11 Section 8b.11 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Practices § 8b.11 Discrimination prohibited. (a) General. (1) No qualified handicapped individual shall,...

  19. 15 CFR 8b.16 - Discrimination prohibited.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Discrimination prohibited. 8b.16 Section 8b.16 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Accessibility § 8b.16 Discrimination prohibited. No qualified handicapped individual shall, because a...

  20. 15 CFR 8b.22 - Academic adjustments.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Academic adjustments. 8b.22 Section 8b... Secondary Education § 8b.22 Academic adjustments. (a) Academic requirements. A recipient to which this subpart applies shall make such modifications to its academic requirements as are necessary to ensure...

  1. 15 CFR 8b.22 - Academic adjustments.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Academic adjustments. 8b.22 Section 8b... Secondary Education § 8b.22 Academic adjustments. (a) Academic requirements. A recipient to which this subpart applies shall make such modifications to its academic requirements as are necessary to ensure...

  2. 15 CFR 8b.5 - Assurances required.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Assurances required. 8b.5 Section 8b.5... Assurances required. (a) Assurances. An applicant for Federal financial assistance to which this part applies shall submit an assurance, on a form specified by the Secretary, that the program or activity will...

  3. 15 CFR 8b.5 - Assurances required.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Assurances required. 8b.5 Section 8b.5... Assurances required. (a) Assurances. An applicant for Federal financial assistance to which this part applies shall submit an assurance, on a form specified by the Secretary, that the program or activity will...

  4. 15 CFR 8b.18 - New construction.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Standards (UFAS) (Appendix A to 41 CFR subpart 101-19.6) shall be deemed to comply with the requirements of... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false New construction. 8b.18 Section 8b.18... construction. (a) Design and construction. Each facility or part of a facility constructed by, on behalf of,...

  5. 15 CFR 8c.40 - Employment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Equal Employment Opportunity Commission in 29 CFR Part 1613, shall apply to employment in federally... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Employment. 8c.40 Section 8c.40... BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF COMMERCE § 8c.40...

  6. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  7. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  8. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  9. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Secondary Education § 8b.25 Nonacademic services. (a) Physical education and athletics. (1) In providing physical education courses and athletics and similar aid, benefits, or services to any of its students, a... offers physical education courses or that operates or sponsors intercollegiate, club, or...

  10. Foothills Parkway Section 8B Final Environmental Report, Volume 2, Appendices A-C

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NW, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this EN there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constricting Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER, which consists of Appendices A, B, and C, assesses the potential geologic impacts of the proposed Section 8B construction, presents the results of the Section 8B soil survey, and describes the water quality studies and analyses performed for the ER. The following summary sections provide information for geology, soils, and water quality.

  11. JT8D engine performance retention

    NASA Technical Reports Server (NTRS)

    James, A. D.; Weisel, D. R.

    1981-01-01

    The attractive performance retention characteristics of the JT8D engine are described. Because of its moderate bypass ratio and turbine temperature, and stiff structural design, the performance retention versus flight cycles of the JT8D engine sets a standard that is difficult for other engines to equal. In addition, the significant benefits of refurbishment of the JT8D engine are presented. Cold section refurbishment offers thrust specific fuel consumption improvements of up to 2 percent and payback in less than a year, making a very attractive investment option for the airlines.

  12. 15 CFR 8c.60 - Communications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Communications. 8c.60 Section 8c.60 Commerce and Foreign Trade Office of the Secretary of Commerce ENFORCEMENT OF NONDISCRIMINATION ON THE... Communications. (a) The agency shall take appropriate steps to ensure effective communication with...

  13. 15 CFR 8c.60 - Communications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Communications. 8c.60 Section 8c.60 Commerce and Foreign Trade Office of the Secretary of Commerce ENFORCEMENT OF NONDISCRIMINATION ON THE... Communications. (a) The agency shall take appropriate steps to ensure effective communication with...

  14. 15 CFR 8c.60 - Communications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Communications. 8c.60 Section 8c.60 Commerce and Foreign Trade Office of the Secretary of Commerce ENFORCEMENT OF NONDISCRIMINATION ON THE... Communications. (a) The agency shall take appropriate steps to ensure effective communication with...

  15. 15 CFR 8c.60 - Communications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Communications. 8c.60 Section 8c.60 Commerce and Foreign Trade Office of the Secretary of Commerce ENFORCEMENT OF NONDISCRIMINATION ON THE... Communications. (a) The agency shall take appropriate steps to ensure effective communication with...

  16. Foothills Parkway Section 8B Final Environmental Report, Volume 6, Appendix N

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER documents the results of the architectural, historical, and cultural resources assessment for the entire Section 8B ROW that was completed in May 1995 to document the architectural, historical, and cultural resources located within the project area. The assessment included evaluation of the potential for cultural (i.e., rural historic) landscapes in the area of the ROW. The assessment showed that one National Register-listed property is located 0.3 mile south of the ROW

  17. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Treatment of students. 8b.21 Section 8b.21 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall,...

  18. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Treatment of students. 8b.21 Section 8b.21 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF DISCRIMINATION... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall,...

  19. Foothills Parkway Section 8B Final Environmental Report, Volume 3, Appendix D

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER inventories the fishes and benthic macroinvertebrates inhabiting the aquatic ecosystems potentially affected by the proposed construction of Section 8B. Stream biological surveys were completed at 31 stream sites during the Fall of 1994. The sampling strategy for both invertebrates and fish was to survey the different taxa from all available habitats. For benthic invertebrates, a standardized qualitative manual collection technique was employed for all 31 stations. For fish

  20. Foothills Parkway Section 8B Final Environmental Report, Volume 5, Appendices J-M

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER consists of Appendices J through M, which describe potential impacts regarding traffic, noise, and aesthetics. The results of the traffic studies described in these appendices resulted in the following conclusions: Unacceptable levels of service will occur on numerous roads within and outside of the park increasingly in the Mure based upon current and future regional growth. However, the results of the traffic assessment indicated that there would be no significant or

  1. A putative, novel coli surface antigen 8B (CS8B) of enterotoxigenic Escherichia coli

    PubMed Central

    Njoroge, Samuel M.; Boinett, Christine J.; Madé, Laure F.; Ouko, Tom T.; Fèvre, Eric M.; Thomson, Nicholas R.; Kariuki, Samuel

    2015-01-01

    Enterotoxigenic Escherichia coli (ETEC) strains harbor multiple fimbriae and pili to mediate host colonization, including the type IVb pilus, colonization factor antigen III (CFA/III). Not all colonization factors are well characterized or known in toxin positive ETEC isolates, which may have an impact identifying ETEC isolates based on molecular screening of these biomarkers. We describe a novel coli surface antigen (CS) 8 subtype B (CS8B), a family of CFA/III pilus, in a toxin producing ETEC isolate from a Kenyan collection. In highlighting the existence of this putative CS, we provide the sequence and specific primers, which can be used alongside other ETEC primers previously described. PMID:26187892

  2. A putative, novel coli surface antigen 8B (CS8B) of enterotoxigenic Escherichia coli.

    PubMed

    Njoroge, Samuel M; Boinett, Christine J; Madé, Laure F; Ouko, Tom T; Fèvre, Eric M; Thomson, Nicholas R; Kariuki, Samuel

    2015-10-01

    Enterotoxigenic Escherichia coli (ETEC) strains harbor multiple fimbriae and pili to mediate host colonization, including the type IVb pilus, colonization factor antigen III (CFA/III). Not all colonization factors are well characterized or known in toxin positive ETEC isolates, which may have an impact identifying ETEC isolates based on molecular screening of these biomarkers. We describe a novel coli surface antigen (CS) 8 subtype B (CS8B), a family of CFA/III pilus, in a toxin producing ETEC isolate from a Kenyan collection. In highlighting the existence of this putative CS, we provide the sequence and specific primers, which can be used alongside other ETEC primers previously described. PMID:26187892

  3. A putative, novel coli surface antigen 8B (CS8B) of enterotoxigenic Escherichia coli.

    PubMed

    Njoroge, Samuel M; Boinett, Christine J; Madé, Laure F; Ouko, Tom T; Fèvre, Eric M; Thomson, Nicholas R; Kariuki, Samuel

    2015-10-01

    Enterotoxigenic Escherichia coli (ETEC) strains harbor multiple fimbriae and pili to mediate host colonization, including the type IVb pilus, colonization factor antigen III (CFA/III). Not all colonization factors are well characterized or known in toxin positive ETEC isolates, which may have an impact identifying ETEC isolates based on molecular screening of these biomarkers. We describe a novel coli surface antigen (CS) 8 subtype B (CS8B), a family of CFA/III pilus, in a toxin producing ETEC isolate from a Kenyan collection. In highlighting the existence of this putative CS, we provide the sequence and specific primers, which can be used alongside other ETEC primers previously described.

  4. F-8C adaptive flight control laws

    NASA Technical Reports Server (NTRS)

    Hartmann, G. L.; Harvey, C. A.; Stein, G.; Carlson, D. N.; Hendrick, R. C.

    1977-01-01

    Three candidate digital adaptive control laws were designed for NASA's F-8C digital flyby wire aircraft. Each design used the same control laws but adjusted the gains with a different adaptative algorithm. The three adaptive concepts were: high-gain limit cycle, Liapunov-stable model tracking, and maximum likelihood estimation. Sensors were restricted to conventional inertial instruments (rate gyros and accelerometers) without use of air-data measurements. Performance, growth potential, and computer requirements were used as criteria for selecting the most promising of these candidates for further refinement. The maximum likelihood concept was selected primarily because it offers the greatest potential for identifying several aircraft parameters and hence for improved control performance in future aircraft application. In terms of identification and gain adjustment accuracy, the MLE design is slightly superior to the other two, but this has no significant effects on the control performance achievable with the F-8C aircraft. The maximum likelihood design is recommended for flight test, and several refinements to that design are proposed.

  5. Foothills Parkway Section 8B Final Environmental Report, Volume 4, Appendices E-I

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west. The major deliverables for the project are listed. From August 1995 through October 1996, NPS, GSMNP, and ORNL staff interacted with Federal Highway Administration staff to develop a conceptual design plan for Section 8B with the intent of protecting critical, resources identified during the ER process to the extent possible. In addition, ORNL arranged for bioengineering experts to discuss techniques that might be employed on Section 8B with NPS, GSMNP, and ORNL staff during September 1996. For the purposes of this ER, there are two basic alternatives under consideration: (1) a build alternative and (2) a no-build alternative. Within the build alternative are a number of options including constructing Section 8B with no interchanges, constructing Section 8B with an interchange at SR 416 or U.S. 321, constructing Section 8B with a spur road on Webb Mountain, and considering operation of Section 8B both before and after the operation of Section 8C. The no-build alternative is considered the no-action alternative and is not to construct Section 8B. This volume of the ER consists of Appendices E through I (all ecological survey reports), which are summarized individually in the sections that follow. The following conclusions result from the completion of these surveys and the ER impact analysis: (1) Forest clearing should be limited as much as possible; (2) Disturbed areas should be replanted with native trees; (3) Drainages should be bridged rather than leveled with cut and fill; (4) For areas of steep slopes and potential erosion

  6. 17 CFR 270.8b-20 - Additional information.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Additional information. 270.8b...) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-20 Additional information. In addition to the information expressly required to be included in a registration statement or report, there...

  7. 15 CFR 8b.20 - Admission and recruitment.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Admission and recruitment. 8b.20... Secondary Education § 8b.20 Admission and recruitment. (a) General. Qualified handicapped may not, on the basis of handicap, be denied admission or be subjected to discrimination in admission or recruitment...

  8. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license or certificate obtained from the United States Coast Guard pursuant to the requirements of 46 CFR 10... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Employment on ships. 8b.15 Section...

  9. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license or certificate obtained from the United States Coast Guard pursuant to the requirements of 46 CFR 10... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Employment on ships. 8b.15 Section...

  10. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license or certificate obtained from the United States Coast Guard pursuant to the requirements of 46 CFR 10... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Employment on ships. 8b.15 Section...

  11. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license or certificate obtained from the United States Coast Guard pursuant to the requirements of 46 CFR 10... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Employment on ships. 8b.15 Section...

  12. 15 CFR 8b.15 - Employment on ships.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Practices § 8b.15 Employment on ships. No qualified handicapped individual possessing an appropriate license or certificate obtained from the United States Coast Guard pursuant to the requirements of 46 CFR 10... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Employment on ships. 8b.15 Section...

  13. Foothills Parkway Section 8B Final Environmental Report, Volume 1

    SciTech Connect

    Blasing, T.J.; Cada, G.F.; Carer, M.; Chin, S.M.; Dickerman, J.A.; Etnier, D.A.; Gibson, R.; Harvey, M.; Hatcher, B.; Lietzske, D.; Mann, L.K.; Mulholland, P.J.; Petrich, C.H.; Pounds, L.; Ranney, J.; Reed, R.M.; Ryan, P.F.; Schweitzer, M.; Smith, D.; Thomason, P.; Wade, M.C.

    1999-07-01

    In 1994, Oak Ridge National Laboratory (ORNL) was tasked by the National Park Service (NPS) to prepare an Environmental Report (ER) for Section 8B of the Foothills Parkway in the Great Smoky Mountains National Park (GSMNP). Section 8B represents 27.7 km (14.2 miles) of a total of 115 km (72 miles) of the planned Foothills Parkway and would connect the Cosby community on the east to the incorporated town of Pittman Center to the west.

  14. 15 CFR 8c.10 - Self-evaluation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Self-evaluation. 8c.10 Section 8c.10... BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF COMMERCE § 8c.10 Self... handicaps or organizations representing individuals with handicaps, to participate in the...

  15. Diminished phosphodiesterase-8B potentiates biphasic insulin response to glucose.

    PubMed

    Dov, Avital; Abramovitch, Eva; Warwar, Nasim; Nesher, Rafael

    2008-02-01

    cAMP activates multiple signal pathways, crucial for the pancreatic beta-cells function and survival and is a major potentiator of insulin release. A family of phosphodiesterases (PDEs) terminate the cAMP signals. We examined the expression of PDEs in rat beta-cells and their role in the regulation of insulin response. Using RT-PCR and Western blot analyses, we identified PDE3A, PDE3B, PDE4B, PDE4D, and PDE8B in rat islets and in INS-1E cells and several possible splice variants of these PDEs. Specific depletion of PDE3A with small interfering (si) RNA (siPDE3A) led to a small (67%) increase in the insulin response to glucose in INS-1E cells but not rat islets. siPDE3A had no effect on the glucagon-like peptide-1 (10 nmol/liter) potentiated insulin response in rat islets. Depletion in PDE8B levels in rat islets using similar technology (siPDE8B) increased insulin response to glucose by 70%, the potentiation being of similar magnitude during the first and second phase insulin release. The siPDE8B-potentiated insulin response was further increased by 23% when glucagon-like peptide-1 was included during the glucose stimulus. In conclusion, PDE8B is expressed in a small number of tissues unrelated to glucose or fat metabolism. We propose that PDE8B, an 3-isobutyl-1-methylxanthine-insensitive cAMP-specific phosphodiesterase, could prove a novel target for enhanced insulin response, affecting a specific pool of cAMP involved in the control of insulin granule trafficking and exocytosis. Finally, we discuss evidence for functional compartmentation of cAMP in pancreatic beta-cells.

  16. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., counseling, financial aid, physical education, athletics, recreation, transportation, other extracurricular, or other post secondary education aid, benefits, or services to which this subpart applies. (b) A... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall,...

  17. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., counseling, financial aid, physical education, athletics, recreation, transportation, other extracurricular, or other post secondary education aid, benefits, or services to which this subpart applies. (b) A... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall,...

  18. 15 CFR 8b.21 - Treatment of students.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., counseling, financial aid, physical education, athletics, recreation, transportation, other extracurricular, or other post secondary education aid, benefits, or services to which this subpart applies. (b) A... Secondary Education § 8b.21 Treatment of students. (a) General. No qualified handicapped student shall,...

  19. JT8D-100 turbofan engine, phase 1. [noise reduction

    NASA Technical Reports Server (NTRS)

    1974-01-01

    The JT8D turbofan engine, widely used in short and medium range transport aircraft, contributes substantially to airport community noise. The jet noise is predominant in the JT8D engine and may be reduced in a modified engine, without loss of thrust, by increasing the airflow to reduce jet velocity. A configuration study evaluated the effects of fan airflow, fan pressure ratio, and bypass ratio on noise, thrust, and fuel comsumption. The cycle selected for the modified engine was based upon an increased diameter, single-stage fan and two additional core engine compressor stages, which replace the existing two-stage fan. Modifications were also made to the low pressure turbine to provide the increased torque required by the larger diameter fan. The resultant JT8D-100 engine models have the following characteristics at take-off thrust, compared to the current JT8D engine: Airflow and bypass ratio are increased, and fan pressure ratio and engine speed are reduced. The resultant engine is also longer, larger in diameter, and heavier than the JT8D base model, but these latter changes are compensated by the increased thrust and decreased fuel comsumption of the modified engine, thus providing the capability for maintaining the performance of the current JT8D-powered aircraft.

  20. 15 CFR 8c.10 - Self-evaluation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Self-evaluation. 8c.10 Section 8c.10...-evaluation. (a) The agency shall, by July 26, 1989, evaluate its current policies and practices, and the... handicaps or organizations representing individuals with handicaps, to participate in the...

  1. The 727/JT8D refan side nacelle airloads

    NASA Technical Reports Server (NTRS)

    Bailey, R. W.; Vadset, H. J.

    1974-01-01

    Airloads on the 727/JT8D refan side engine nacelle are presented. These consist of surface static pressure distributions from two low speed wind tunnel tests. External nacelle surface pressures are from testing of a flow-through, body mounted nacelle model, and internal inlet surface pressures are from performance testing of a forced air inlet model. The method for obtaining critical airloads on nacelle components and a representative example are discussed.

  2. 15 CFR 8c.50 - Program accessibility: Existing facilities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES CONDUCTED BY THE DEPARTMENT OF COMMERCE § 8c.50 Program accessibility: Existing facilities. (a) General. The agency shall operate each program or activity so that the program or activity, when viewed in its entirety, is readily accessible to and usable...

  3. WASP-12b AND HAT-P-8b are members of triple star systems

    SciTech Connect

    Bechter, Eric B.; Crepp, Justin R.; Matthews, Christopher T.; Ngo, Henry; Knutson, Heather A.; Batygin, Konstantin; Johnson, John Asher; Hinkley, Sasha; Muirhead, Philip S.; Montet, Benjamin T.; Morton, Timothy D.; Howard, Andrew W.

    2014-06-10

    We present high spatial resolution images that demonstrate that WASP-12b and HAT-P-8b orbit the primary stars of hierarchical triple star systems. In each case, two distant companions with colors and brightnesses consistent with M dwarfs co-orbit the hot Jupiter planet host as well as one another. Our adaptive optics images spatially resolve the secondary around WASP-12, previously identified by Bergfors et al. and Crossfield et al. into two distinct sources separated by 84.3 ± 0.6 mas (21 ± 3 AU). We find that the secondary to HAT-P-8, also identified by Bergfors et al., is in fact composed of two stars separated by 65.3 ± 0.5 mas (15 ± 1 AU). Our follow-up observations demonstrate physical association through common proper motion. HAT-P-8 C has a particularly low mass, which we estimate to be 0.18 ± 0.02 M {sub ☉} using photometry. Due to their hierarchy, WASP-12 BC and HAT-P-8 BC will enable the first dynamical mass determination for hot Jupiter stellar companions. These previously well studied planet hosts now represent higher-order multi-star systems with potentially complex dynamics, underscoring the importance of diffraction-limited imaging and providing additional context for understanding the migrant population of transiting hot Jupiters.

  4. WASP-12b and HAT-P-8b are Members of Triple Star Systems

    NASA Astrophysics Data System (ADS)

    Bechter, Eric B.; Crepp, Justin R.; Ngo, Henry; Knutson, Heather A.; Batygin, Konstantin; Hinkley, Sasha; Muirhead, Philip S.; Johnson, John Asher; Howard, Andrew W.; Montet, Benjamin T.; Matthews, Christopher T.; Morton, Timothy D.

    2014-06-01

    We present high spatial resolution images that demonstrate that WASP-12b and HAT-P-8b orbit the primary stars of hierarchical triple star systems. In each case, two distant companions with colors and brightnesses consistent with M dwarfs co-orbit the hot Jupiter planet host as well as one another. Our adaptive optics images spatially resolve the secondary around WASP-12, previously identified by Bergfors et al. and Crossfield et al. into two distinct sources separated by 84.3 ± 0.6 mas (21 ± 3 AU). We find that the secondary to HAT-P-8, also identified by Bergfors et al., is in fact composed of two stars separated by 65.3 ± 0.5 mas (15 ± 1 AU). Our follow-up observations demonstrate physical association through common proper motion. HAT-P-8 C has a particularly low mass, which we estimate to be 0.18 ± 0.02 M ⊙ using photometry. Due to their hierarchy, WASP-12 BC and HAT-P-8 BC will enable the first dynamical mass determination for hot Jupiter stellar companions. These previously well studied planet hosts now represent higher-order multi-star systems with potentially complex dynamics, underscoring the importance of diffraction-limited imaging and providing additional context for understanding the migrant population of transiting hot Jupiters.

  5. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Ammonium salts of higher fatty acids... Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids...

  6. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 24 2011-07-01 2011-07-01 false Ammonium salts of higher fatty acids... Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids...

  7. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Ammonium salts of higher fatty acids... Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids...

  8. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Ammonium salts of higher fatty acids... Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids...

  9. 40 CFR 180.1284 - Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Ammonium salts of higher fatty acids... Ammonium salts of higher fatty acids (C8-C18 saturated; C8-C12 unsaturated); exemption from the requirement of a tolerance. Ammonium salts of C8-C18 saturated and C8-C12 unsaturated higher fatty acids...

  10. 15 CFR 8b.6 - Remedial action, voluntary action, and self-evaluation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... self-evaluation. 8b.6 Section 8b.6 Commerce and Foreign Trade Office of the Secretary of Commerce... DEPARTMENT OF COMMERCE General Provisions § 8b.6 Remedial action, voluntary action, and self-evaluation. (a... or this part and where another recipient exercises control over the recipient that has...

  11. 15 CFR 8b.24 - Financial and employment assistance to students.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... students. 8b.24 Section 8b.24 Commerce and Foreign Trade Office of the Secretary of Commerce PROHIBITION OF... Secondary Education § 8b.24 Financial and employment assistance to students. (a) Provision of financial... person that provides assistance to any of the recipient's students in a manner that discriminates...

  12. Extremal black attractors in 8D maximal supergravity

    SciTech Connect

    Drissi, L. B; Hassani, F. Z; Jehjouh, H.; Saidi, E. H

    2010-05-15

    Motivated by the new higher D-supergravity solutions on intersecting attractors obtained by Ferrara et al. in [Phys. Rev. D 79, 065031 (2009)], we focus in this paper on 8D maximal supergravity with moduli space (SL(3,R)/SO(3))x(SL(2,R)/SO(2)) and study explicitly the attractor mechanism for various configurations of extremal black p-branes (antibranes) with the typical near horizon geometries AdS{sub p+2}xS{sup m}xT{sup 6-p-m} and p=0, 1, 2, 3, 4; 2{<=}m{<=}6. Interpretations in terms of wrapped M2 and M5 branes of the 11D M-theory on 3-torus are also given.

  13. Status of the JT8D refan noise reduction program

    NASA Technical Reports Server (NTRS)

    Kester, J. D.

    1974-01-01

    The present work describes measures being taken to suppress jet noise in the JT8D engine and reports on some preliminary acoustic tests to evaluate noise characteristics of the engine. To reduce noise generation without sacrificing other essential engine performance or durability characteristics, the two stage fan will be replaced by a larger-diameter single stage fan. Refanned engines have an increased bypass ratio that contributes to the reduction of jet exhaust noise. Advanced acoustical design features will be incorporated in the larger, single-stage fan, and acoustic treatment will be incorporated in both the engine and the nacelle. First tests have shown that engine component noise levels are about as predicted and the acoustic treatment sections tested to date are providing predicted levels of attenuation. Further work is required to demonstrate engine performance goals and compatibility with 727 and DC-9 installations.

  14. JT8D high pressure compressor performance improvement

    NASA Technical Reports Server (NTRS)

    Gaffin, W. O.

    1981-01-01

    An improved performance high pressure compressor with potential application to all models of the JT8D engine was designed. The concept consisted of a trenched abradable rubstrip which mates with the blade tips for each of the even rotor stages. This feature allows tip clearances to be set so blade tips run at or near the optimum radius relative to the flowpath wall, without the danger of damaging the blades during transients and maneuvers. The improved compressor demonstrated thrust specific fuel consumption and exhaust gas temperature improvements of 1.0 percent and at least 10 C over the takeoff and climb power range at sea level static conditions, compared to a bill-of-material high pressure compressor. Surge margin also improved 4 percentage points over the high power operating range. A thrust specific fuel consumption improvement of 0.7 percent at typical cruise conditions was calculated based on the sea level test results.

  15. F-8C digital CCV flight control laws

    NASA Technical Reports Server (NTRS)

    Hartmann, G. L.; Hauge, J. A.; Hendrick, R. C.

    1976-01-01

    A set of digital flight control laws were designed for the NASA F-8C digital fly-by-wire aircraft. The control laws emphasize Control Configured Vehicle (CCV) benefits. Specific pitch axis objectives were improved handling qualities, angle-of-attack limiting, gust alleviation, drag reduction in steady and maneuvering flight, and a capability to fly with reduced static stability. The lateral-directional design objectives were improved Dutch roll damping and turn coordination over a wide range in angle-of-attack. An overall program objective was to explore the use of modern control design methodilogy to achieve these specific CCV benefits. Tests for verifying system integrity, an experimental design for handling qualities evaluation, and recommended flight test investigations were specified.

  16. Fiscal year 1995 laboratory scale studies of Cs elution in Tank 8D-1 and sludge dissolution in tank 8D-2

    SciTech Connect

    Sills, J.A.; Patello, G.K.; Roberts, J.S.; Wiemers, K.D.; Elmore, M.R.; Richmond, W.G.; Russell, R.L.

    1996-04-01

    During Phase I of West Valley Demonstration project waste remediation, an estimated 95% of the zeolite currently in tank 8D-1 will be transferred to tank 8D-2, leaving behind residual Cs-loaded zeolite which will require treatment to remove the Cs. After phase I vitrification, tank 8D-2 will contain residual waste from PUREX and THOREX and spent Cs-loaded zeolite. The residual waste will require treatment. Oxalic acid has been proposed for eluting Cs from zeolite in tank 8D-1 and dissolving radionuclides in tank 8D-2. Laboratory tests were performed to determine optimum Cs elution and sludge dissolution conditions and to evaluate effects of multiple contacts, long-term contacts, presence of corrosion products, lack of agitation, temperature of tank contents, and oxalic acid concentration. Mild steel corrosion tests were also conducted.

  17. Global gene profiling of aging lungs in Atp8b1 mutant mice

    PubMed Central

    Soundararajan, Ramani; Stearns, Timothy M.; Czachor, Alexander; Fukumoto, Jutaro; Turn, Christina; Westermann-Clark, Emma; Breitzig, Mason; Tan, Lee; Lockey, Richard F.; King, Benjamin L.; Kolliputi, Narasaiah

    2016-01-01

    Objective Recent studies implicate cardiolipin oxidation in several age-related diseases. Atp8b1 encoding Type 4 P-type ATPases is a cardiolipin transporter. Mutation in Atp8b1 gene or inflammation of the lungs impairs the capacity of Atp8b1 to clear cardiolipin from lung fluid. However, the link between Atp8b1 mutation and age-related gene alteration is unknown. Therefore, we investigated how Atp8b1 mutation alters age-related genes. Methods We performed Affymetrix gene profiling of lungs isolated from young (7-9 wks, n=6) and aged (14 months, 14 M, n=6) C57BL/6 and Atp8b1 mutant mice. In addition, Ingenuity Pathway Analysis (IPA) was performed. Differentially expressed genes were validated by quantitative real-time PCR (qRT-PCR). Results Global transcriptome analysis revealed 532 differentially expressed genes in Atp8b1 lungs, 157 differentially expressed genes in C57BL/6 lungs, and 37 overlapping genes. IPA of age-related genes in Atp8b1 lungs showed enrichment of Xenobiotic metabolism and Nrf2-mediated signaling pathways. The increase in Adamts2 and Mmp13 transcripts in aged Atp8b1 lungs was validated by qRT-PCR. Similarly, the decrease in Col1a1 and increase in Cxcr6 transcripts was confirmed in both Atp8b1 mutant and C57BL/6 lungs. Conclusion Based on transcriptome profiling, our study indicates that Atp8b1 mutant mice may be susceptible to age-related lung diseases. PMID:27689529

  18. 15 CFR 8b.7 - Designation of responsible employee and adoption of grievance procedures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... and adoption of grievance procedures. 8b.7 Section 8b.7 Commerce and Foreign Trade Office of the... adoption of grievance procedures. (a) Designation of responsible employee. A recipient, other than a small...) Adoption of grievance procedures. A recipient, other than a small recipient, shall adopt...

  19. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good... report or any portion thereof may be prepared by any similar process which, in the opinion of...

  20. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good... report or any portion thereof may be prepared by any similar process which, in the opinion of...

  1. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good... portion thereof may be prepared by any similar process which, in the opinion of the Commission,...

  2. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES AND... Requirements as to paper, printing and language. (a) Registration statements and reports shall be filed on good.... (d) The body of all printed registration statements and reports and all notes to financial...

  3. 17 CFR 270.8b-12 - Requirements as to paper, printing and language.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... and reports shall be in the English language. If any exhibit or other paper or document filed with a... English language. (f) Where a registration statement or report is distributed through an electronic medium..., printing and language. 270.8b-12 Section 270.8b-12 Commodity and Securities Exchanges SECURITIES...

  4. The YAV-8B simulation and modeling. Volume 2: Program listing

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Detailed mathematical models of varying complexity representative of the YAV-8B aircraft are defined and documented. These models are used in parameter estimation and in linear analysis computer programs while investigating YAV-8B aircraft handling qualities. Both a six degree of freedom nonlinear model and a linearized three degree of freedom longitudinal and lateral directional model were developed. The nonlinear model is based on the mathematical model used on the MCAIR YAV-8B manned flight simulator. This simulator model has undergone periodic updating based on the results of approximately 360 YAV-8B flights and 8000 hours of wind tunnel testing. Qualified YAV-8B flight test pilots have commented that the handling qualities characteristics of the simulator are quite representative of the real aircraft. These comments are validated herein by comparing data from both static and dynamic flight test maneuvers to the same obtained using the nonlinear program.

  5. Results of acoustic testing of the JT8D-109 refan engines

    NASA Technical Reports Server (NTRS)

    Burdsall, E. A.; Brochu, F. P.; Scaramella, V. M.

    1975-01-01

    A JT8D engine was modified to reduce jet noise levels by 6-8 PNdB at takeoff power without increasing fan generated noise levels. Designated the JT8D-109, the modified engines featured a larger single stage fan, and acoustic treatment in the fan discharge ducts. Noise levels were measured on an outdoor test facility for eight engine/acoustic treatment configurations. Compared to the baseline JT8D, the fully treated JT8D-109 showed reductions of 6 PNdB at takeoff, and 11 PNdB at a typical approach power setting.

  6. Structural mechanism for the regulation of HCN ion channels by the accessory protein TRIP8b

    PubMed Central

    DeBerg, Hannah A.; Bankston, John R.; Rosenbaum, Joel C.; Brzovic, Peter S.; Zagotta, William N.; Stoll, Stefan

    2015-01-01

    Summary Hyperpolarization-activated cyclic nucleotide-gated (HCN) ion channels underlie the cationic Ih current present in many neurons. The direct binding of cAMP to HCN channels increases the rate and extent of channel opening and results in a depolarizing shift in the voltage dependence of activation. TRIP8b is an accessory protein that regulates the cell surface expression and dendritic localization of HCN channels and reduces the cyclic nucleotide dependence of these channels. Here we use electron paramagnetic resonance (EPR) to show that TRIP8b binds to the apo state of the cyclic nucleotide-binding domain (CNBD) of HCN2 channels without changing the overall domain structure. With EPR and nuclear magnetic resonance (NMR), we locate TRIP8b relative to the HCN channel and identify the binding interface on the CNBD. These data provide a structural framework for understanding how TRIP8b regulates the cyclic nucleotide dependence of HCN channels. PMID:25800552

  7. Full genomic analyses of human rotavirus strains possessing the rare P[8]b VP4 subtype.

    PubMed

    Ghosh, Souvik; Paul, Shyamal Kumar; Yamamoto, Dai; Nagashima, Shigeo; Kobayashi, Nobumichi

    2011-08-01

    Rotaviruses with the P[8] VP4 genotype are a major cause of acute infantile diarrhea. The P[8] genotype is classified into two genetically distinct subtypes, P[8]a and P[8]b. Most of the P[8] strains belong to subtype P[8]a, whilst P[8]b strains are rare. To date, the whole genomes of a few P[8]a strains have been analyzed, whilst there are no reports on full genomic analysis of the P[8]b strains. To determine the genetic makeup of the rare P[8]b strains and their overall genetic relatedness to the P[8]a strains, the present study analyzed the full genomes of a human G9P[8]b strain, MMC38, and a G1P[8]b strain, MMC71, detected in Bangladesh in 2005. By nucleotide sequence identities and phylogenetic analyses, strains MMC38 and MMC71 exhibited a human rotavirus Wa-like genotype constellation. Except for the VP4 gene, all the genes of strains MMC38 and MMC71 were closely related to cognate genes of the contemporary and more recent human Wa-like G1P[8]a, G9P[8]a, G11P[8]a, G11P[25], G12P[6] and G12P[8]a strains, including those from Bangladesh. Therefore, strains MMC38 and MMC71 possessed the genetically distinct P[8]b VP4 gene on a common human Wa-like genetic backbone, pointing towards their possible origin from reassortment events between common human Wa-like strains and unidentified rotavirus strains possessing the rare P[8]b-like VP4 gene. Since strains with this stable Wa-like genetic backbone can spread rapidly, and it is not certain as to whether the current rotavirus vaccines will be equally efficacious against the P[8]b strains as the P[8]a strains, proper detection of P[8]b strains and their whole genomic analyses might be of public health significance. To our knowledge, the present study is the first report on full genomic analysis of the rare P[8]b rotavirus strains. PMID:21640848

  8. The phospholipid flippase ATP8B1 mediates apical localization of the cystic fibrosis transmembrane regulator.

    PubMed

    van der Mark, Vincent A; de Jonge, Hugo R; Chang, Jung-Chin; Ho-Mok, Kam S; Duijst, Suzanne; Vidović, Dragana; Carlon, Marianne S; Oude Elferink, Ronald P J; Paulusma, Coen C

    2016-09-01

    Progressive familial intrahepatic cholestasis type 1 (PFIC1) is caused by mutations in the gene encoding the phospholipid flippase ATP8B1. Apart from severe cholestatic liver disease, many PFIC1 patients develop extrahepatic symptoms characteristic of cystic fibrosis (CF), such as pulmonary infection, sweat gland dysfunction and failure to thrive. CF is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), a chloride channel essential for epithelial fluid transport. Previously it was shown that CFTR transcript levels were strongly reduced in livers of PFIC1 patients. Here we have investigated the hypothesis that ATP8B1 is important for proper CFTR expression and function. We analyzed CFTR expression in ATP8B1-depleted intestinal and pulmonary epithelial cell lines and assessed CFTR function by measuring short-circuit currents across transwell-grown ATP8B1-depleted intestinal T84 cells and by a genetically-encoded fluorescent chloride sensor. In addition, we studied CFTR surface expression upon induction of CFTR transcription. We show that CFTR protein levels are strongly reduced in the apical membrane of human ATP8B1-depleted intestinal and pulmonary epithelial cell lines, a phenotype that coincided with reduced CFTR activity. Apical membrane insertion upon induction of ectopically-expressed CFTR was strongly impaired in ATP8B1-depleted cells. We conclude that ATP8B1 is essential for correct apical localization of CFTR in human intestinal and pulmonary epithelial cells, and that impaired CFTR localization underlies some of the extrahepatic phenotypes observed in ATP8B1 deficiency. PMID:27301931

  9. BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions

    PubMed Central

    Whittle, Andrew J.; Carobbio, Stefania; Martins, Luís; Slawik, Marc; Hondares, Elayne; Vázquez, María Jesús; Morgan, Donald; Csikasz, Robert I.; Gallego, Rosalía; Rodriguez-Cuenca, Sergio; Dale, Martin; Virtue, Samuel; Villarroya, Francesc; Cannon, Barbara; Rahmouni, Kamal; López, Miguel; Vidal-Puig, Antonio

    2012-01-01

    Summary Thermogenesis in brown adipose tissue (BAT) is fundamental to energy balance and is also relevant for humans. Bone morphogenetic proteins (BMPs) regulate adipogenesis, and, here, we describe a role for BMP8B in the direct regulation of thermogenesis. BMP8B is induced by nutritional and thermogenic factors in mature BAT, increasing the response to noradrenaline through enhanced p38MAPK/CREB signaling and increased lipase activity. Bmp8b−/− mice exhibit impaired thermogenesis and reduced metabolic rate, causing weight gain despite hypophagia. BMP8B is also expressed in the hypothalamus, and Bmp8b−/− mice display altered neuropeptide levels and reduced phosphorylation of AMP-activated protein kinase (AMPK), indicating an anorexigenic state. Central BMP8B treatment increased sympathetic activation of BAT, dependent on the status of AMPK in key hypothalamic nuclei. Our results indicate that BMP8B is a thermogenic protein that regulates energy balance in partnership with hypothalamic AMPK. BMP8B may offer a mechanism to specifically increase energy dissipation by BAT. PMID:22579288

  10. Asymptotic normalization coefficients (nuclear vertex constants) for p + 7Be → 8B and the direct 7Be( p, γ)8B astrophysical S factors at solar energies

    NASA Astrophysics Data System (ADS)

    Igamov, S. B.; Yarmukhamedov, R.

    2008-10-01

    A new analysis of the precise experimental astrophysical S factors for the direct-capture reaction 7Be( p, γ)8B [A.J. Junghans et al., Phys. Rev. C 68, 065803 (2003) and L.T. Baby et al., Phys. Rev. C 67, 065805 (2003)] is carried out on the basis of a modified two-body potential approach in which the direct astrophysical S factor, S 17( E), is expressed in terms of the asymptotic normalization constants for p + 7Be → 8B and two additional conditions are involved to verify the peripheral character of the reaction under consideration. The Woods-Saxon potential form is used for the bound-( p + 7Be)-state wave function and for p 7Be-scattering wave function. New estimates are obtained for the “indirectly measured” values of the asymptotic normalization constants (the nuclear vertex constants) for the p + 7Be → 8B and S 17( E) at E ≤ 115 keV, including E = 0. These values of S 17( E) and asymptotic normalization constants have been used for obtaining the indirectly measured values of the s-wave average scattering length and the p-wave effective-range parameters for p 7Be scattering.

  11. First-Principles Study of the Electronic Structure and Bonding Properties of X8C46 and X8B6C40 (X: Li, Na, Mg, Ca) Carbon Clathrates

    NASA Astrophysics Data System (ADS)

    KoleŻyński, Andrzej; Szczypka, Wojciech

    2016-03-01

    Results from theoretical analysis of the crystal structure, electronic structure, and bonding properties of C46 and B6C40 carbon clathrates doped with selected alkali and alkaline earth metals cations (Li, Na, Mg, Ca) are presented. The ab initio calculations were performed by means of the WIEN2k package (full potential linearized augmented plane wave method (FP-LAPW) within density functional theory (DFT)) with PBESol and modified Becke-Johnson exchange-correlation potentials used in geometry optimization and electronic structure calculations, respectively. The bonding properties were analyzed by applying Bader's quantum theory of atoms in molecules formalism to the topological properties of total electron density obtained from ab initio calculations. Analysis of the results obtained (i.a. equilibrium geometry, equation of state, cohesive energy, band structure, density of states—both total and projected on to particular atoms, and topological properties of bond critical points and net charges of topological atoms) is presented in detail.

  12. ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization

    PubMed Central

    Bruurs, Lucas J.M.; Donker, Lisa; Zwakenberg, Susan; Zwartkruis, Fried J.; Begthel, Harry; Knisely, A.S.; Posthuma, George; van de Graaf, Stan F.J.; Paulusma, Coen C.

    2015-01-01

    During yeast cell polarization localization of the small GTPase, cell division control protein 42 homologue (Cdc42) is clustered to ensure the formation of a single bud. Here we show that the disease-associated flippase ATPase class I type 8b member 1 (ATP8B1) enables Cdc42 clustering during enterocyte polarization. Loss of this regulation results in increased apical membrane size with scattered apical recycling endosomes and permits the formation of more than one apical domain, resembling the singularity defect observed in yeast. Mechanistically, we show that to become apically clustered, Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids provided by ATP8B1. Disturbing this interaction, either by ATP8B1 depletion or by introduction of a Cdc42 mutant defective in lipid binding, increases Cdc42 mobility and results in apical membrane enlargement. Re-establishing Cdc42 clustering, by tethering it to the apical membrane or lowering its diffusion, restores normal apical membrane size in ATP8B1-depleted cells. We therefore conclude that singularity regulation by Cdc42 is conserved between yeast and human and that this regulation is required to maintain healthy tissue architecture. PMID:26416959

  13. Autosomal-dominant striatal degeneration is caused by a mutation in the phosphodiesterase 8B gene.

    PubMed

    Appenzeller, Silke; Schirmacher, Anja; Halfter, Hartmut; Bäumer, Sebastian; Pendziwiat, Manuela; Timmerman, Vincent; De Jonghe, Peter; Fekete, Klára; Stögbauer, Florian; Lüdemann, Peter; Hund, Margret; Quabius, Elgar Susanne; Ringelstein, E Bernd; Kuhlenbäumer, Gregor

    2010-01-01

    Autosomal-dominant striatal degeneration (ADSD) is an autosomal-dominant movement disorder affecting the striatal part of the basal ganglia. ADSD is characterized by bradykinesia, dysarthria, and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present. Using genetic linkage analysis, we have mapped the causative genetic defect to a 3.25 megabase candidate region on chromosome 5q13.3-q14.1. A maximum LOD score of 4.1 (Theta = 0) was obtained at marker D5S1962. Here we show that ADSD is caused by a complex frameshift mutation (c.94G>C+c.95delT) in the phosphodiesterase 8B (PDE8B) gene, which results in a loss of enzymatic phosphodiesterase activity. We found that PDE8B is highly expressed in the brain, especially in the putamen, which is affected by ADSD. PDE8B degrades cyclic AMP, a second messenger implied in dopamine signaling. Dopamine is one of the main neurotransmitters involved in movement control and is deficient in Parkinson disease. We believe that the functional analysis of PDE8B will help to further elucidate the pathomechanism of ADSD as well as contribute to a better understanding of movement disorders.

  14. ATP8B1-mediated spatial organization of Cdc42 signaling maintains singularity during enterocyte polarization.

    PubMed

    Bruurs, Lucas J M; Donker, Lisa; Zwakenberg, Susan; Zwartkruis, Fried J; Begthel, Harry; Knisely, A S; Posthuma, George; van de Graaf, Stan F J; Paulusma, Coen C; Bos, Johannes L

    2015-09-28

    During yeast cell polarization localization of the small GTPase, cell division control protein 42 homologue (Cdc42) is clustered to ensure the formation of a single bud. Here we show that the disease-associated flippase ATPase class I type 8b member 1 (ATP8B1) enables Cdc42 clustering during enterocyte polarization. Loss of this regulation results in increased apical membrane size with scattered apical recycling endosomes and permits the formation of more than one apical domain, resembling the singularity defect observed in yeast. Mechanistically, we show that to become apically clustered, Cdc42 requires the interaction between its polybasic region and negatively charged membrane lipids provided by ATP8B1. Disturbing this interaction, either by ATP8B1 depletion or by introduction of a Cdc42 mutant defective in lipid binding, increases Cdc42 mobility and results in apical membrane enlargement. Re-establishing Cdc42 clustering, by tethering it to the apical membrane or lowering its diffusion, restores normal apical membrane size in ATP8B1-depleted cells. We therefore conclude that singularity regulation by Cdc42 is conserved between yeast and human and that this regulation is required to maintain healthy tissue architecture. PMID:26416959

  15. Autosomal-Dominant Striatal Degeneration Is Caused by a Mutation in the Phosphodiesterase 8B Gene

    PubMed Central

    Appenzeller, Silke; Schirmacher, Anja; Halfter, Hartmut; Bäumer, Sebastian; Pendziwiat, Manuela; Timmerman, Vincent; De Jonghe, Peter; Fekete, Klára; Stögbauer, Florian; Lüdemann, Peter; Hund, Margret; Quabius, Elgar Susanne; Ringelstein, E. Bernd; Kuhlenbäumer, Gregor

    2010-01-01

    Autosomal-dominant striatal degeneration (ADSD) is an autosomal-dominant movement disorder affecting the striatal part of the basal ganglia. ADSD is characterized by bradykinesia, dysarthria, and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present. Using genetic linkage analysis, we have mapped the causative genetic defect to a 3.25 megabase candidate region on chromosome 5q13.3-q14.1. A maximum LOD score of 4.1 (Θ = 0) was obtained at marker D5S1962. Here we show that ADSD is caused by a complex frameshift mutation (c.94G>C+c.95delT) in the phosphodiesterase 8B (PDE8B) gene, which results in a loss of enzymatic phosphodiesterase activity. We found that PDE8B is highly expressed in the brain, especially in the putamen, which is affected by ADSD. PDE8B degrades cyclic AMP, a second messenger implied in dopamine signaling. Dopamine is one of the main neurotransmitters involved in movement control and is deficient in Parkinson disease. We believe that the functional analysis of PDE8B will help to further elucidate the pathomechanism of ADSD as well as contribute to a better understanding of movement disorders. PMID:20085714

  16. Nuclear Structure of 8B Studied by Proton Resonance Scatterings on 7Be

    NASA Astrophysics Data System (ADS)

    Yamaguchi, H.; Wakabayashi, Y.; Hayakawa, S.; Amadio, G.; Kubono, S.; Fujikawa, H.; Saito, A.; He, J. J.; Teranishi, T.; Kwon, Y. K.; Nishimura, S.; Togano, Y.; Iwasa, N.; Inafuku, K.; Niikura, M.; Binh, D. N.; Khiem, L. H.

    2008-05-01

    A new measurement of the proton resonance scattering on 7Be was performed up to the excitation energy of 6.8 MeV using the low-energy RI beam facility CRIB (CNS Radioactive Ion Beam separator) at the Center for Nuclear Study (CNS) of the University of Tokyo. The excitation function of 8B above 3.5 MeV was successfully measured for the first time, providing important information about the reaction rate of 7Be(p,γ)8B, which is the key reaction in the solar 8B neutrino production. For more intensive experimental studies with RI beams, the development of a cryogenic gas target system is ongoing at CNS. In this paper a preliminary result of the 7Be experiment and the present status of the development of the target system are presented.

  17. Phosphodiesterase 8B and cyclic AMP signaling in the adrenal cortex.

    PubMed

    Leal, Leticia Ferro; Szarek, Eva; Faucz, Fabio; Stratakis, Constantine A

    2015-09-01

    Bilateral adrenocortical hyperplasia (BAH) in humans and mice has been recently linked to phosphodiesterase (PDE) 8B (PDE8B) and 11 (PDE11A) defects. These findings have followed the discovery that defects of primary genes of the cyclic monophosphatase (cAMP) signaling pathway, such as guanine nucleotide binding alpha subunit and PRKAR1A, are involved in the pathogenesis of BAH in humans; complete absence of Prkar1a in the adrenal cortex of mice also led to pathology that mimicked the human disease. Here, we review the most recent findings in human and mouse studies on PDE8B, a cAMP-specific PDE that appears to be highly expressed in the adrenal cortex and whose deficiency may underlie predisposition to BAH and possibly other human diseases. PMID:25971952

  18. Nuclear Structure of {sup 8}B Studied by Proton Resonance Scatterings on {sup 7}Be

    SciTech Connect

    Yamaguchi, H.; Wakabayashi, Y.; Hayakawa, S.; Amadio, G.; Kubono, S.; Fujikawa, H.; Niikura, M.; Binh, D. N.; Saito, A.; He, J. J.; Teranishi, T.; Kwon, Y. K.; Nishimura, S.; Togano, Y.; Iwasa, N.; Inafuku, K.; Khiem, L. H.

    2008-05-21

    A new measurement of the proton resonance scattering on {sup 7}Be was performed up to the excitation energy of 6.8 MeV using the low-energy RI beam facility CRIB (CNS Radioactive Ion Beam separator) at the Center for Nuclear Study (CNS) of the University of Tokyo. The excitation function of {sup 8}B above 3.5 MeV was successfully measured for the first time, providing important information about the reaction rate of {sup 7}Be(p,{gamma}){sup 8}B, which is the key reaction in the solar {sup 8}B neutrino production. For more intensive experimental studies with RI beams, the development of a cryogenic gas target system is ongoing at CNS. In this paper a preliminary result of the {sup 7}Be experiment and the present status of the development of the target system are presented.

  19. YAV-8B reaction control system bleed and control power usage in hover and transition

    NASA Technical Reports Server (NTRS)

    Borchers, Paul F.; Moralez, Ernesto, III; Merrick, Vernon K.; Stortz, Michael W.

    1994-01-01

    Using a calibrated Rolls-Royce Pegasus engine and existing aircraft instrumentation and pressure taps, total and individual nozzle reaction control system (RCS) bleed flow rates have been measured on a YAV-8B Harrier during typical short takeoff, transition, hover, and vertical landing maneuvers. RCS thrust forces were calculated from RCS nozzle total pressure measurements, and control power was determined from the moments produced by these thrusts and the aircraft's moments of inertia. These data document the characteristics of the YAV-8B RCS with its basic stability augmentation system (SAS) engaged. Advanced control system designs for the YAV-8B can be compared to the original SAS based on the total bleed use and the percentage of available bleed used. In addition, the peak and mean values of the bleed and control power data can be used for sizing the reaction controls for a future short takeoff and vertical landing (STOVL) aircraft.

  20. Analysis of Human TAAR8 and Murine Taar8b Mediated Signaling Pathways and Expression Profile

    PubMed Central

    Mühlhaus, Jessica; Dinter, Juliane; Nürnberg, Daniela; Rehders, Maren; Depke, Maren; Golchert, Janine; Homuth, Georg; Yi, Chun-Xia; Morin, Silke; Köhrle, Josef; Brix, Klaudia; Tschöp, Matthias; Kleinau, Gunnar; Biebermann, Heike

    2014-01-01

    The thyroid hormone derivative 3-iodothyronamine (3-T1AM) exerts metabolic effects in vivo that contradict known effects of thyroid hormones. 3-T1AM acts as a trace amine-associated receptor 1 (TAAR1) agonist and activates Gs signaling in vitro. Interestingly, 3-T1AM-meditated in vivo effects persist in Taar1 knockout-mice indicating that further targets of 3-T1AM might exist. Here, we investigated another member of the TAAR family, the only scarcely studied mouse and human trace-amine-associated receptor 8 (Taar8b, TAAR8). By RT-qPCR and locked-nucleic-acid (LNA) in situ hybridization, Taar8b expression in different mouse tissues was analyzed. Functionally, we characterized TAAR8 and Taar8b with regard to cell surface expression and signaling via different G-protein-mediated pathways. Cell surface expression was verified by ELISA, and cAMP accumulation was quantified by AlphaScreen for detection of Gs and/or Gi/o signaling. Activation of G-proteins Gq/11 and G12/13 was analyzed by reporter gene assays. Expression analyses revealed at most marginal Taar8b expression and no gender differences for almost all analyzed tissues. In heart, LNA-in situ hybridization demonstrated the absence of Taar8b expression. We could not identify 3-T1AM as a ligand for TAAR8 and Taar8b, but both receptors were characterized by a basal Gi/o signaling activity, a so far unknown signaling pathway for TAARs. PMID:25391046

  1. Measurement of the reaction ^7Be(p,γ)^8B with a solid radioactive target

    NASA Astrophysics Data System (ADS)

    Greife, Uwe

    2000-04-01

    The reaction ^7Be(p,γ)^8B is regarded as a key to the understanding of the solar neutrino spectrum and thus most probably to the understanding of new neutrino properties. This contribution describes an experiment measuring the absolute cross section of ^7Be(p,γ)^8B with a solid radioactive target in the energy range between 350 keV and 2.6 MeV. The experiment was performed at the Dynamitron Tandem Laboratory of the Ruhr-Universitaet Bochum, Germany.

  2. Probing the nuclear structure of {sup 8}B and {sup 19}C

    SciTech Connect

    Baumann, T.; Geissel, H.; Aumann, T.; Cortina-Gil, D.; Hellstroem, M.; Muenzenberg, G.; Nickel, F.; Scheidenberger, C.; Schwab, W.; Suemmerer, K.; Winkler, M.; Lenske, H.; Markenroth, K.; Axelsson, L.; Jonson, B.; Nilsson, T.; Smedberg, M. H.; Zhukov, M. V.; Bergmann, U.; Riisager, K.

    1998-12-21

    The nuclei {sup 8}B and {sup 19}C were investigated in breakup reactions at relativistic energies. The fragment separator FRS at GSI was used as an energy-loss spectrometer to measure the longitudinal momentum distributions of the breakup fragments after one-nucleon removal. For the case of {sup 8}B, the measured momentum distribution is in agreement with our previous experiment, but in this measurement the momentum range extends up to 175 MeV/c in the projectile frame. For {sup 18}C fragments from the breakup of {sup 19}C, we extract a much larger momentum width than the value obtained in a measurement at lower projectile energies.

  3. 15 CFR 8b.10 - Effect of state or local law or other requirements and effect of employment opportunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Effect of state or local law or other requirements and effect of employment opportunities. 8b.10 Section 8b.10 Commerce and Foreign Trade Office of... PROGRAMS OPERATED BY THE DEPARTMENT OF COMMERCE General Provisions § 8b.10 Effect of state or local law...

  4. Trafficking and Gating of Hyperpolarization-activated Cyclic Nucleotide-gated Channels Are Regulated by Interaction with Tetratricopeptide Repeat-containing Rab8b-interacting Protein (TRIP8b) and Cyclic AMP at Distinct Sites*

    PubMed Central

    Han, Ye; Noam, Yoav; Lewis, Alan S.; Gallagher, Johnie J.; Wadman, Wytse J.; Baram, Tallie Z.; Chetkovich, Dane M.

    2011-01-01

    Ion channel trafficking and gating are often influenced by interactions with auxiliary subunits. Tetratricopeptide repeat-containing Rab8b-interacting protein (TRIP8b) is an auxiliary subunit for neuronal hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. TRIP8b interacts directly with two distinct sites of HCN channel pore-forming subunits to control channel trafficking and gating. Here we use mutagenesis combined with electrophysiological studies to define and distinguish the functional importance of the HCN/TRIP8b interaction sites. Interaction with the last three amino acids of the HCN1 C terminus governed the effect of TRIP8b on channel trafficking, whereas TRIP8b interaction with the HCN1 cyclic nucleotide binding domain (CNBD) affected trafficking and gating. Biochemical studies revealed that direct interaction between TRIP8b and the HCN1 CNBD was disrupted by cAMP and that TRIP8b binding to the CNBD required an arginine residue also necessary for cAMP binding. In accord, increasing cAMP levels in cells antagonized the up-regulation of HCN1 channels mediated by a TRIP8b construct binding the CNBD exclusively. These data illustrate the distinct roles of the two TRIP8b-HCN interaction domains and suggest that TRIP8b and cAMP may directly compete for binding the HCN CNBD to control HCN channel gating, kinetics, and trafficking. PMID:21504900

  5. 17 CFR 270.8b-21 - Information unknown or not available.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 17 Commodity and Securities Exchanges 4 2014-04-01 2014-04-01 false Information unknown or not... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-21 Information unknown or not... registrant. If any required information is unknown and not reasonably available to the registrant,...

  6. 17 CFR 270.8b-21 - Information unknown or not available.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 17 Commodity and Securities Exchanges 3 2013-04-01 2013-04-01 false Information unknown or not... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-21 Information unknown or not... registrant. If any required information is unknown and not reasonably available to the registrant,...

  7. 17 CFR 270.8b-21 - Information unknown or not available.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Information unknown or not... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-21 Information unknown or not... registrant. If any required information is unknown and not reasonably available to the registrant,...

  8. 17 CFR 270.8b-21 - Information unknown or not available.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 17 Commodity and Securities Exchanges 3 2012-04-01 2012-04-01 false Information unknown or not... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-21 Information unknown or not... registrant. If any required information is unknown and not reasonably available to the registrant,...

  9. The expansion of Phytophthora clade 8b: three new species associated with winter grown vegetable crops.

    PubMed

    Bertier, L; Brouwer, H; de Cock, A W A M; Cooke, D E L; Olsson, C H B; Höfte, M

    2013-12-01

    Despite its association with important agricultural crops, Phytophthora clade 8b is a poorly studied group of species. The clade currently consists of three officially described species (Phytophthora porri, P. brassicae and P. primulae) that are host-specific pathogens of leek, cabbages and Primula spp., respectively. However, over the past few decades, several other clade 8b-like Phytophthoras have been found on a variety of different host plants that were all grown at low temperatures in winter seasons. In this study, a collection of 30 of these isolates was subjected to a phylogenetic study using two loci (the rDNA ITS region and the mitochondrial cox1 gene). This analysis revealed a clear clustering of isolates according to their host plants. To verify whether these isolates belong to separate species, a detailed morphological study was conducted. On the basis of genetic and morphological differences and host specificity, we now present the official description of three new species in clade 8b: Phytophthora cichorii sp. nov., P. dauci sp. nov. and P. lactucae sp. nov. Two other groups of isolates (Phytophthora taxon castitis and Phytophthora taxon parsley) might also represent new species but the data available at this time are insufficient for an official description. This brings Phytophthora clade 8b to a group of six species that are all host-specific, slow-growing and specifically infect herbaceous crops at low temperatures.

  10. 7Be(p,(gamma))8B S-factor From Ab Initio Wave Functions

    SciTech Connect

    Navratil, P; Bertulani, C; Caurier, E

    2005-08-15

    Nuclear structure of {sup 7}Be, {sup 8}B and {sup 7,8}Li is studied within the ab initio no-core shell model (NCSM). Starting from the high-precision CD-Bonn 2000 nucleon-nucleon (NN) interaction, wave functions of {sup 7}Be and {sup 8}B bound states are obtained in basis spaces up to 10{h_bar}{Omega} and used to calculate channel cluster form factors (overlap integrals) of the {sup 8}B ground state with {sup 7}Be+p. Due to the use of the harmonic oscillator (HO) basis, the overlap integrals have incorrect asymptotic properties. We fix this problem in two alternative ways. First, by a Woods-Saxon (WS) potential solution fit to the interior of the NCSM overlap integrals. Second, by a direct matching with the Whittaker function. The corrected overlap integrals are then used for the {sup 7}Be(p,{gamma}){sup 8}B S-factor calculation. We study the convergence of the S-factor with respect to the NCSM HO frequency and the model space size. Our S-factor results are in agreement with recent direct measurement data.

  11. 17 CFR 270.8b-10 - Requirements as to proper form.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 17 Commodity and Securities Exchanges 4 2014-04-01 2014-04-01 false Requirements as to proper form... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-10 Requirements as to proper form... by the Commission, as in effect on the date of filing. Any such statement or report shall be...

  12. 17 CFR 270.8b-10 - Requirements as to proper form.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Requirements as to proper form... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-10 Requirements as to proper form... by the Commission, as in effect on the date of filing. Any such statement or report shall be...

  13. 17 CFR 270.8b-11 - Number of copies; signatures; binding.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Number of copies; signatures... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.8b-11 Number of copies; signatures; binding. (a) Three complete copies of each registration statement or report, including exhibits and...

  14. A 727/JT8D-100 series engine exhaust system propulsion performance model test

    NASA Technical Reports Server (NTRS)

    Haugan, W. J.; Kern, P. R. A.

    1974-01-01

    The results are presented from testing one-eighth scale models of the Pratt and Whitney aircraft reference and Boeing nozzles for the JT8D-100 series mixed flow engines. The objective of the test was to obtain the nozzle velocity and flow coefficients for the reference configurations and compare these with the Boeing configurations which incorporated a longer splitter between the fan and primary flows. A further comparison was made between the JT8D-100 series nozzles and the Boeing JT8D-9/727 production nozzle performance. A statistical analysis was used to compare configurations which showed the performance (velocity coefficient) of the reference and the Boeing configuration was the same for the JT8D-109. It also showed no difference between reference and the Boeing configuration for the JT8D-115 and no difference for the JT8D-117 nozzles. Bypass ratio (match) was shown to be equally dependent on splitter position as on nozzle area within the range investigated. The nozzles were very similar in flow coefficient within an engine family. Excellent profile data was recorded. The effects of swirl on the nozzle performance was examined and found to degrade the velocity and flow coefficients.

  15. Program for refan JT8D engine design, fabrication and test, phase 2

    NASA Technical Reports Server (NTRS)

    Glass, J. A.; Zimmerman, E. S.; Scaramella, V. M.

    1975-01-01

    The objective of the JT8D refan program was to design, fabricate, and test certifiable modifications of the JT8D engine which would reduce noise generated by JT8D powered aircraft. This was to be accomplished without affecting reliability and maintainability, at minimum retrofit cost, and with no performance penalty. The mechanical design, engine performance and stability characteristics at sea-level and altitude, and the engine noise characteristics of the test engines are documented. Results confirmed the structural integrity of the JT8D-109. Engine operation was stable throughout the airplane flight envelope. Fuel consumption of the test engines was higher than that required to meet the goal of no airplane performance penalty, but the causes were identified and corrected during a normal pre-certification engine development program. Compared to the baseline JT8D-109 engine, the acoustically treated JT8D-109 engine showed noise reductions of 6 PNdB at takeoff and 11 PNdB at a typical approach power setting.

  16. Determination of YAV-8B Reaction Control System bleed flow usage

    NASA Technical Reports Server (NTRS)

    Borchers, Paul F.; Moralez, Ernesto, III; Merrick, Vernon K.; Stortz, Michael W.; Eames, David J. H.

    1992-01-01

    Using a calibrated Rolls-Royce Pegasus engine, total Reaction Control System (RCS) bleed flow rates have been measured on a YAV-8B Harrier during typical short takeoff, transition, hover and vertical landing maneuvers. Using existing aircraft instrumentation and pressure taps located in the RCS ducts, bleed flow rates at each RCS valve were also measured directly during flight and ground tests. These data were compared with the calibrated engine data and with the RCS part of a YAV-8B mathematical model used in piloted simulation at NASA Ames Research Center. Areas of disagreement were small, being confined to the estimation of closed RCS valve leakages and the modeling of the RCS butterfly valve pressure losses.

  17. Measurement of the 8B Solar Neutrino Flux with KamLAND

    SciTech Connect

    Abe, S.; Furuno, K.; Gando, A.; Gando, Y.; Ichimura, K.; Ikeda, H.; Inoue, K.; Kibe, Y.; Kimura, W.; Kishimoto, Y.; Koga, M.; Minekawa, Y.; Mitsui, T.; Morikawa, T.; Nagai, N.; Nakajima, K.; Nakamura, K.; Nakamura, M.; Narita, K.; Shimizu, I.; Shimizu, Y.; Shirai, J.; Suekane, F.; Suzuki, A.; Takahashi, H.; Takahashi, N.; Takemoto, Y.; Tamae, K.; Watanabe, H.; Xu, B.D.; Yabumoto, H.; Yonezawa, E.; Yoshida, H.; Yoshida, S.; Enomoto, S.; Kozlov, A.; Murayama, H.; Grant, C.; Keefer, G.; McKee, D.; Piepke, A.; Banks, T.I.; Bloxham, T.; Detwiler, J.A.; Freedman, S.J.; Fujikawa, B.K.; Han, K.; Kadel, R.; O'Donnell, T.; Steiner, H.M.; Winslow, L.A.; Dwyer, D.A.; Mauger, C.; McKeown, R.D.; Zhang, C.; Berger, B.E.; Lane, C.E.; Maricic, J.; Miletic, T.; Batygov, M.; Learned, J.G.; Matsuno, S.; Pakvasa, S.; Sakai, M.; Horton-Smith, G.A.; Tang, A.; Downum, K.E.; Gratta, G.; Tolich, K.; Efremenko, Y.; Kamyshkov, Y.; Perevozchikov, O.; Karwowski, H.J.; Markoff, D.M.; Tornow, W.; Heeger, K.M.; Piquemal, F.; Ricol, J.-S.; Decowski, M.P.

    2011-06-04

    We report a measurement of the neutrino-electron elastic scattering rate from {sup 8}B solar neutrinos based on a 123 kton-day exposure of KamLAND. The background-subtracted electron recoil rate, above a 5.5-MeV analysis threshold is 1.49 {+-} 0.14(stat) {+-} 0.17(syst) events per kton-day. Interpreted as due to a pure electron flavor flux with a {sup 8}B neutrino spectrum, this corresponds to a spectrum integrated flux of 2.77 {+-} 0.26(stat) {+-} 0.32(syst) x 10{sup 6} cm{sup -2}s{sup -1}. The analysis threshold is driven by {sup 208}Tl present in the liquid scintillator, and the main source of systematic uncertainty is due to background from cosmogenic {sup 11}Be. The measured rate is consistent with existing measurements and with standard solar model predictions which include matter-enhanced neutrino oscillation.

  18. Complete Genome Sequence of Fowl Aviadenovirus Serotype 8b Isolated in South America

    PubMed Central

    Calderón, Katherine; Chumbe, Ana; Montesinos, Ricardo; Montalván, Ángela; González, Armando E.; Icochea, Eliana; Fernández-Díaz, Manolo

    2016-01-01

    We present here the complete genome sequence of fowl aviadenovirus E (FAdV-E) serotype 8b strain FV211-16, isolated from chickens with inclusion body hepatitis in Peru. Genome comparisons with other FAdV-E strains revealed identities of 84.9 to 97.1% and the presence of 9 and 2 unique amino acid mutations in hexon and fiber proteins, respectively.

  19. A lower radius and mass for the transiting extrasolar planet HAT-P-8 b

    NASA Astrophysics Data System (ADS)

    Mancini, L.; Southworth, J.; Ciceri, S.; Fortney, J. J.; Morley, C. V.; Dittmann, J. A.; Tregloan-Reed, J.; Bruni, I.; Barbieri, M.; Evans, D. F.; D'Ago, G.; Nikolov, N.; Henning, Th.

    2013-03-01

    Context. The extrasolar planet HAT-P-8 b was thought to be one of the more inflated transiting hot Jupiters. Aims: By using new and existing photometric data, we computed precise estimates of the physical properties of the system. Methods: We present photometric observations comprising eleven light curves covering six transit events, obtained using five medium-class telescopes and telescope-defocussing technique. One transit was simultaneously obtained through four optical filters, and two transits were followed contemporaneously from two observatories. We modelled these and seven published datasets using the jktebop code. The physical parameters of the system were obtained from these results and from published spectroscopic measurements. In addition, we investigated the theoretically-predicted variation of the apparent planetary radius as a function of wavelength, covering the range 330-960 nm. Results: We find that HAT-P-8 b has a significantly lower radius (1.321 ± 0.037 RJup) and mass (1.275 ± 0.053 MJup) compared to previous estimates (1.50-0.06+0.08 R_{Jup} and 1.52-0.16+0.18 M_{Jup} respectively). We also detect a radius variation in the optical bands that, when compared with synthetic spectra of the planet, may indicate the presence of a strong optical absorber, perhaps TiO and VO gases, near the terminator of HAT-P-8 b. Conclusions: These new results imply that HAT-P-8 b is not significantly inflated, and that its position in the planetary mass-radius diagram is congruent with those of many other transiting extrasolar planets. Full Table 2 is only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/551/A11

  20. Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes.

    PubMed

    Kobayashi, Shintaro; Suzuki, Tadaki; Kawaguchi, Akira; Phongphaew, Wallaya; Yoshii, Kentaro; Iwano, Tomohiko; Harada, Akihiro; Kariwa, Hiroaki; Orba, Yasuko; Sawa, Hirofumi

    2016-03-18

    West Nile virus (WNV) particles assemble at and bud into the endoplasmic reticulum (ER) and are secreted from infected cells through the secretory pathway. However, the host factor related to these steps is not fully understood. Rab proteins, belonging to the Ras superfamily, play essential roles in regulating many aspects of vesicular trafficking. In this study, we sought to determine which Rab proteins are involved in intracellular trafficking of nascent WNV particles. RNAi analysis revealed that Rab8b plays a role in WNV particle release. We found that Rab8 and WNV antigen were colocalized in WNV-infected human neuroblastoma cells, and that WNV infection enhanced Rab8 expression in the cells. In addition, the amount of WNV particles in the supernatant of Rab8b-deficient cells was significantly decreased compared with that of wild-type cells. We also demonstrated that WNV particles accumulated in the recycling endosomes in WNV-infected cells. In summary, these results suggest that Rab8b is involved in trafficking of WNV particles from recycling endosomes to the plasma membrane. PMID:26817838

  1. Evolution of substellar 'brown' dwarfs and the evolutionary status of VB8B

    NASA Technical Reports Server (NTRS)

    Stringfellow, Guy S.

    1986-01-01

    Detailed evolutionary calculations for stars near and below the minimum main sequence (MS) mass are presented, and the results are compared to VB8B. The standard set of stellar equations are solved, and the influence of both high and low grain opacities is assessed. All masses less than 0.08 solar are found to evolve to 0.0001 solar luminosities in a billion years or less. Both 0.08 solar mass and 0.075 solar mass stars become MS stars after five billion years, but with luminosities substantially below 0.0001 solar. The best fit to VB8B is 0.06 + or - 0.02 solar mass, but less than 0.8 solar mass. Depending upon its very precise mass, VB8B can be either a substellar brown dwarf with an age of 0.3-3 billion years, or an MS star with an age anywhere from two billion years to a Hubble time.

  2. The Characterization of the Cool and Eccentric Exoplanet WASP-8b with Spitzer

    NASA Astrophysics Data System (ADS)

    Cubillos, Patricio; Harrington, J.; Madhusudhan, N.; Stevenson, K.; Hardy, R.; Blecic, J.; Anderson, D.; Hardin, M.; Campo, C.

    2012-10-01

    WASP-8b is one of the coldest hot-Jupiter planets observed during secondary eclipse (when the planet passes behind the star) with the Spitzer Space Telescope. We present the our observations of WASP-8b at the 3.6, 4.5, and 8.0-micron wavebands with the Spitzer's IRAC instrument, during secondary eclipse. We will show the resulting light curves of our infrared observations, determining the planet-to-star flux ratios. With this spectral information we further characterized the planet's dayside atmosphere, constraining its chemical composition, recognizing the absence of a thermal inversion, and estimating the energy redistribution regime over its surface. Although having a equilibrium temperature is only 950K, the large eccentricity of the orbit (e=0.31) should make the dayside temperature of WASP-8b oscillate with an amplitude of hundreds of degrees. We modeled these temporal variation of the temperature over the surface of the planet and set constrains on the rotational angular velocity and radiative timescale of the planet, based in the observed hemisphere-averaged brightness temperature during secondary eclipse. This planet is also dynamically interesting since it orbits the primary star of a binary system. Along with a large eccentricity, suggests the presence of an unseen planetary companion. A precise determination of the eclipse mid-times will help to constrain the orbit of such companion.

  3. Atomization and combustion characteristics of antimisting fuels using JT8D and air-boost injectors

    NASA Technical Reports Server (NTRS)

    Kennedy, J. B.; Florentino, A. J.

    1986-01-01

    The atomization levels of antimisting fuels are presently determined for a JT8D fuel injector, a low emission airblast JT8D injector, and an air-boost injector, at operating conditions simulating engine operating conditions. The effects of the use of antimisting kerosene (AMK) on component performance are also studied in the case of an in-service JT8D engine. The use of the AMK fuel causes a decline in the quality of the spray, most notably as a large increase in the Sauter mean diameter for all three injector types. In addition, the idle patternation data obtained indicate that the low emission injector fuel distribution changed from a hollow cone Jet A spray having no fuel at its center to a semihollow spray cone in the case of AMK; this change could disrupt the combustor primary zone recirculation pattern.

  4. Pollution reduction technology program for class T4(JT8D) engines

    NASA Technical Reports Server (NTRS)

    Roberts, R.; Fiorentino, A. J.; Diehl, L. A.

    1977-01-01

    The technology required to develop commercial gas turbine engines with reduced exhaust emissions was demonstrated. Can-annular combustor systems for the JT8D engine family (EPA class T4) were investigated. The JT8D turbofan engine is an axial-flow, dual-spool, moderate-bypass-ratio design. It has a two-stage fan, a four-stage low-pressure compressor driven by a three-stage low-pressure turbine, and a seven-stage high-pressure compressor driven by a single-stage high-pressure turbine. A cross section of the JT8D-17 showing the mechanical configuration is given. Key specifications for this engine are listed.

  5. Summary and analysis of performance and stability characteristics of the refan JT8D-109 engine

    NASA Technical Reports Server (NTRS)

    Sanberg, K. G.; Mogielnicki, R. M.; Davis, J. C.; Scaramella, V. M.

    1975-01-01

    The refan JT8D-109 performance and stability characteristics are reported as determined from sea level testing, altitude testing, and DC-9 flight testing. The test results are summarized as follows: (1) TSFC at SLS achieved design goal of 12.66 percent reduction. (2) TSFC at altitude average cruise power 0.5 percent higher than design goal, (3) TSFC at altitude maximum cruise power 1.7-3.7 percent higher than design goal, (4) ground starting consistent with JT8D-9 base engine, (5) successful flight starts without starter assist, (6) transient surge margin equivalent to JT8D-9, (7) stable engine operation with inlet distortion, and (8) stable engine operation during snap acceleration and deceleration. A flight idle setting is required for acceptable aborted-landing go-around acceleration time due to increase in low-rotor moment of inertia, and a performance improvement program should be conducted as part of any future certification program.

  6. Structural and electronic properties of neutral and charged Ca 8C 12 metal carbides

    NASA Astrophysics Data System (ADS)

    Chen, Gang; Peng, Qi; Kawazoe, Yoshiyuki

    2011-05-01

    This Letter reports our detailed first-principles study on neutral and charged Ca8C12 clusters. Except a compact structure owning geometrical characters of the CaC2 bulk, all the other low-lying structures within 0.2 eV in relative total energy are hollow configurations. Most of the low-lying structures are calculated to be magnetic. The ground states are a D3d flat structure, a compact structure, and a D3d hollow cage-like structure for Ca8C12, Ca8C12-, and Ca8C12+ clusters, respectively. The net charge on Ca ion, except the dangling one of the compact structure, is calculated to be around +1.4e.

  7. High-Temperature Performance of Cast CF8C-Plus Austenitic Stainless Steel

    SciTech Connect

    Maziasz, Philip J; Pint, Bruce A

    2011-01-01

    Covers and casings of small to medium size gas turbines can be made from cast austenitic stainless steels, including grades such as CF8C, CF3M, or CF10M. Oak Ridge National Laboratory and Caterpillar have developed a new cast austenitic stainless steel, CF8C-Plus, which is a fully austenitic stainless steel, based on additions of Mn and N to the standard Nb-stabilized CF8C steel grade. The Mn addition improves castability, as well as increases the alloy solubility for N, and both Mn and N synergistically act to boost mechanical properties. CF8C-Plus steel has outstanding creep-resistance at 600-900 C, which compares well with Ni-based superalloys such as alloys X, 625, 617, and 230. CF8C-Plus also has very good fatigue and thermal fatigue resistance. It is used in the as-cast condition, with no additional heat-treatments. While commercial success for CF8C-Plus has been mainly for diesel exhaust components, this steel can also be considered for gas turbine and microturbine casings. The purposes of this paper are to demonstrate some of the mechanical properties, to update the long-term creep-rupture data, and to present new data on the high-temperature oxidation behavior of these materials, particularly in the presence of water vapor.

  8. Conserved POU/OCT- and GATA-binding sites in 5'-flanking promoter region of mammalian WNT8B orthologs.

    PubMed

    Katoh, Masuko; Katoh, Masaru

    2007-05-01

    WNT family members are secreted-type glycoproteins regulating cell fate, planar cell polarity, cell adhesion, and cell movement. WNT signals are context-dependently transduced to the canonical pathway for the transcriptional up-regulation of MYC, CCND1, FGF20, JAG1, WISP1 and DKK1 genes, and also to the non-canonical pathway for the activation of RHOA, JNK, PKC, NFAT and NLK signaling cascades. We cloned and characterized the wild-type human WNT8B, while another group the aberrant human WNT8B with Gly230Ala and Arg284Leu amino-acid substitutions. Although WNT8B is undetectable in normal adult tissues by using Northern blot analyses, WNT8B is expressed in gastric cancer, pancreatic cancer, colorectal cancer, breast cancer, and embryonal tumors. Here, comparative integromics on WNT8B orthologs were investigated by using bioinformatics (Techint) and human intelligence (Humint). Cow Wnt8b gene was identified within NW_001494361.1 genome sequence. Predicted sequence XM_582222.3 was an artificial cow Wnt8b with aberrant prediction for the first exon. Cow Wnt8b complete coding sequence was found to encode a 350-amino-acid protein, which showed 96.9% total-amino-acid identity with human WNT8B. Comparative proteomics revealed that N-terminal signal peptide, 22 Cys residues, two Asn-linked glycosylation sites, Gly230, and Arg284 of human WNT8B were conserved among mammalian WNT8B orthologs. Comparative genomics revealed that POU/OCT- and GATA-binding sites in the 5'-flanking promoter region were conserved among human, chimpanzee, cow, mouse, and rat WNT8B orthologs. In silico expression analyses revealed that human WNT8B was expressed in embryoid body derived from embryonic stem (ES) cells, hepatocyte progenitors derived from ES cells, fetal brain, diffuse-type gastric cancer, colorectal cancer, prostate cancer, and ovarian fibrotheoma. Based on the expression profiles of POU and GATA family transcription factors, it was revealed that WNT8B expression in hepatocyte

  9. DC-9 flight demonstration program with refanned JT8D engines. Volume 1: Summary

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The design, analysis, fabrication, and ground and flight testing of DC-9 airframe/nacelle hardware with prototype JT8D-109 engines are discussed. The installation of the JT8D-109 engine on the DC-9 Refan airplane required new or modified hardware for the pylon, nacelle, and fuselage. The acoustic material used in the nose cowl was bonded aluminum honeycomb sandwich and the exhaust duct acoustic material was Inconel 625 Stresskin. The sea level static, standard day bare engine takeoff thrust, the cruise TSFC and the maximum available cruise thrust for the JT8D-109 engine were compared with those of the JT8D-9 engine. The range capabilities of the DC-9 Refan and the production DC-9 airplane were also compared. The Refan airplane demonstrated flight characteristics similar to the production DC-9-30 and satisfied airworthiness requirements. Flyover noise levels were determined for the DC-9 Refan and the DC-9 C-9A airplane for takeoff and landing conditions. Cost estimates were also made.

  10. Fiscal year 1996 laboratory scale studies of cesium elution in tank 8D-1

    SciTech Connect

    Russell, R.L.; Patello, G.K.; Sills, J.A.

    1996-09-01

    This report details work performed as part of the West Valley Support Project (WVSP) by Pacific Northwest National Laboratory (PNNL). This work is intended to support residual waste removal during high-level waste (HLW) tank stabilization activities to be performed by the West valley Demonstration Project (WVDP). The HLW originated from a now inactive commercial nuclear fuel-reprocessing plant at West Valley, New York. It is stored in a carbon-steel tank designated 8D-2. Cesium-loaded zeolite was generated by a supernatant decontamination process involving ion exchange. The exchange columns and the spent zeolite are stored in a carbon-steel tank designated 8D-1. During the vitrification phase of the WVDP waste remediation, and estimated 95 percent of the zeolite will be transferred from tank 8D-1 to tank 8D- 2. The remaining cesium-loaded zeolite will require treatment to remove the highly radioactive cesium. This report summarizes the findings of laboratory experiments. The primary objectives of these experiments were: to refine the optimum process conditions for use of oxalic acid to elute cesium from zeolite; minimize iron dissolution during cesium elution; investigation of the effect of neutralization on cesium elution; determination of effects of zeolite particle size on cesium elution; and determine if aluminum can be used as an indicator for cesium in solution.

  11. Scale model testing of the jet noise characteristics of the JT8D refan engine nozzle system

    NASA Technical Reports Server (NTRS)

    Reed, D. H.

    1974-01-01

    The results are presented of static scale model acoustic tests of the nozzle system for the JT8D-9 baseline engine and candidate nozzle systems for JT8D-109, JT8D-115, and JT8D-117 refan engines. The objective of these tests was to determine the jet noise benefit of the three refan engine cycles relative to the baseline JT8D-9, and to provide acoustic information toward selection of the optimum primary-secondary area match and centerbody contour for the refan engine cycles. One of the nozzle configurations was tested with and without simulated turbine exit swirl to determine what effect, if any, swirl has no jet noise. The JT8D-109 cycle was found to afford approximately 9 db (0ASPL) jet noise reduction relative to the JT8D-9 when compared on an equal static thrust basis. The JT8D-115 and JT8D-117 afford an 8 db and 6 db reduction, respectively, relative to the JT8D-9, at equal static thrust. Turbine exit swirl was found to have no significant effect on the jet noise of the JT8D-109 nozzle system.

  12. DETECTING GRAVITY MODES IN THE SOLAR {sup 8} B NEUTRINO FLUX

    SciTech Connect

    Lopes, Ilídio; Turck-Chièze, Sylvaine E-mail: ilopes@uevora.pt

    2014-09-10

    The detection of gravity modes produced in the solar radiative zone has been a challenge in modern astrophysics for more than 30 yr and their amplitude in the core is not yet determined. In this Letter, we develop a new strategy to look for standing gravity modes through solar neutrino fluxes. We note that due to a resonance effect, the gravity modes of low degree and low order have the largest impact on the {sup 8} B neutrino flux. The strongest effect is expected to occur for the dipole mode with radial order 2, corresponding to periods of about 1.5 hr. These standing gravity waves produce temperature fluctuations that are amplified by a factor of 170 in the boron neutrino flux for the corresponding period, in consonance with the gravity modes. From current neutrino observations, we determine that the maximum temperature variation due to the gravity modes in the Sun's core is smaller than 5.8 × 10{sup –4}. This study clearly shows that due to their high sensitivity to the temperature, the {sup 8} B neutrino flux time series is an excellent tool to determine the properties of gravity modes in the solar core. Moreover, if gravity mode footprints are discovered in the {sup 8} B neutrino flux, this opens a new line of research to probe the physics of the solar core as non-standing gravity waves of higher periods cannot be directly detected by helioseismology but could leave their signature on boron neutrino or on other neutrino fluxes.

  13. An 8-b 1.3-MHz successive-approximation A/D converter

    NASA Technical Reports Server (NTRS)

    Hadidi, KH.; Tso, Vincent S.; Temes, Gabor C.

    1990-01-01

    A new successive-approximation A/D converter is described. It combines a string of equal-valued polysilicon resistors and a set of ratioed capacitors in a unique circuit configuration so that a high sampling rate is achieved. The comparator is realized by a chopper-stabilized amplifier to reduce the effect of the offset voltages of MOS amplifiers. The converter performs an 8-b monotonic conversion with a differential nonlinearity less than 1 LSB in 770 nsec. The die area is 3750 sq mil. This new conversion technique can also be utilized in a pipelined A/D converter (Temes, 1985) and enables it to achieve high speed.

  14. The New Structural Materials Science Beamlines BL8A and 8B at Photon Factory

    SciTech Connect

    Nakao, A.; Sugiyama, H.; Koyama, A.; Watanabe, K.

    2010-06-23

    BL8A and 8B are new beamlines for structural materials science at Photon Factory. The primary characteristics of both beamlines are similar. The incident beam is monochromatized by the Si(111) double-flat crystal monochromator and focused at the sample position by a Rh-coated bent cylindrical quartz mirror. The Weissenberg-camera-type imaging-plate (IP) diffractometers were installed. The X-ray diffraction experiments for structural studies of strongly correlated materials, such as transition metals, molecular conductors, endohedral fullerenes, nano-materials, etc, are conducted at these stations.

  15. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... citations affecting Form N-8B-3, see the List of CFR Sections Affected, which appears in the Finding Aids... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.13 Form N..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  16. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... citations affecting Form N-8B-3, see the List of CFR Sections Affected, which appears in the Finding Aids... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.13 Form N..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  17. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... citations affecting Form N-8B-3, see the List of CFR Sections Affected, which appears in the Finding Aids... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.13 Form N..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  18. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... citations affecting Form N-8B-3, see the List of CFR Sections Affected, which appears in the Finding Aids... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.13 Form N..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  19. 17 CFR 274.13 - Form N-8B-3, registration statement of unincorporated management investment companies currently...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... citations affecting Form N-8B-3, see the List of CFR Sections Affected, which appears in the Finding Aids... (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF 1940 Registration Statements § 274.13 Form N..., pursuant to section 8(b) of the Investment Company Act of 1940, by unincorporated management...

  20. A long pentraxin-3-derived pentapeptide for the therapy of FGF8b-driven steroid hormone-regulated cancers.

    PubMed

    Giacomini, Arianna; Matarazzo, Sara; Pagano, Katiuscia; Ragona, Laura; Rezzola, Sara; Corsini, Michela; Di Salle, Emanuela; Presta, Marco; Ronca, Roberto

    2015-05-30

    Fibroblast growth factor-8b (FGF8b) affects the epithelial/stromal compartments of steroid hormone-regulated tumors by exerting an autocrine activity on cancer cells and a paracrine pro-angiogenic function, thus contributing to tumor progression. The FGF8b/FGF receptor (FGFR) system may therefore represent a target for the treatment of steroid hormone-regulated tumors. The soluble pattern recognition receptor long pentraxin-3 (PTX3) binds various FGFs, including FGF2 and FGF8b, thus inhibiting the angiogenic and tumorigenic activity of androgen-regulated tumor cells. Nevertheless, the complex/proteinaceous structure of PTX3 hampers its pharmacological exploitation. In this context, the acetylated pentapeptide Ac-ARPCA-NH2 (ARPCA), corresponding to the N-terminal amino acid sequence PTX3(100-104), was identified as a minimal FGF2-binding peptide able to antagonize the biological activity of FGF2. Here, we demonstrate that ARPCA binds FGF8b and inhibits its capacity to form FGFR1-mediated ternary complexes with heparan sulphate proteoglycans. As a FGF8b antagonist, ARPCA inhibits FGFR1 activation and signalling in endothelial cells, hampering the angiogenic activity exerted in vitro and in vivo by FGF8b. Also, ARPCA suppresses the angiogenic and tumorigenic potential of prototypic androgen/FGF8b-dependent Shionogi 115 mammary carcinoma cells and of androgen/FGF8b/FGF2-dependent TRAMP-C2 prostate cancer cells. In conclusion, ARPCA represents a novel FGF8b antagonist with translational implications for the therapy of steroid hormone-regulated tumors.

  1. Adsorption of Procion Red MX 8B using spent tea leaves as adsorbent

    NASA Astrophysics Data System (ADS)

    Heraldy, Eddy; Osa, Riesta Ramdhaniyati; Suryanti, Venty

    2016-02-01

    The adsorption of Procion Red MX 8B using spent tea leaves (STL) as adsorbent, has been studied by batch adsorption technique. The adsorbent was activated by NaOH 4% for 24 hours for delignification process. The adsorbent was characterized using FTIR to indetify the functional groups of cellulose was shown by uptake -OH, C-H and C-O. The optimum conditions of adsorption experiments were achieved when pH was set as 6 with contact time of 75 minutes and capacity of adsorption was 3.28 mg/g. The equilibrium data were fitted to Langmuir and Isotherm Freundlichs. The kinetic models, pseudo first order and pseudo second order were employed to describe the adsorption mechanism. The experimental results showed that the pseudo second order equation was the best model that described the adsorption behavior with the coefficient of correlation (R2) was equal higher than 0.99 The results suggested that STL had high potential to be used as effective adsorbent for Procion Red MX 8B removal.

  2. WASP-8b: CHARACTERIZATION OF A COOL AND ECCENTRIC EXOPLANET WITH SPITZER

    SciTech Connect

    Cubillos, Patricio; Harrington, Joseph; Hardy, Ryan A.; Blecic, Jasmina; Hardin, Matthew; Campo, Christopher J.; Madhusudhan, Nikku; Stevenson, Kevin B.; Anderson, David R.

    2013-05-01

    WASP-8b has 2.18 times Jupiter's mass and is on an eccentric (e = 0.31) 8.16 day orbit. With a time-averaged equilibrium temperature of 948 K, it is one of the least-irradiated hot Jupiters observed with the Spitzer Space Telescope. We have analyzed six photometric light curves of WASP-8b during secondary eclipse observed in the 3.6, 4.5, and 8.0 {mu}m Infrared Array Camera bands. The eclipse depths are 0.113% {+-} 0.018%, 0.069% {+-} 0.007%, and 0.093% {+-} 0.023%, respectively, giving respective brightness temperatures of 1552, 1131, and 938 K. We characterized the atmospheric thermal profile and composition of the planet using a line-by-line radiative transfer code and a Markov-chain Monte Carlo sampler. The data indicated no thermal inversion, independently of any assumption about chemical composition. We noted an anomalously high 3.6 {mu}m brightness temperature (1552 K); by modeling the eccentricity-caused thermal variation, we found that this temperature is plausible for radiative timescales less than {approx}10{sup 2} hr. However, as no model spectra fit all three data points well, the temperature discrepancy remains as an open question.

  3. 7Be(p,gamma)8B S-factor from Ab Initio Wave Functions

    SciTech Connect

    Navratil, P; Bertulani, C A; Caurier, E

    2006-10-12

    There has been a significant progress in ab initio approaches to the structure of light nuclei. Starting from realistic two- and three-nucleon interactions the ab initio no-core shell model (NCSM) predicts low-lying levels in p-shell nuclei. It is a challenging task to extend ab initio methods to describe nuclear reactions. We present here a brief overview of the first steps taken toward nuclear reaction applications. In particular, we discuss our calculation of the {sup 7}Be(p,{gamma}){sup 8}B S-factor. We also present our first results of the {sup 3}He({alpha},{gamma}){sup 7}Be S-factor and of the S-factor of the mirror reaction {sup 3}H({alpha},{gamma}){sup 7}Li. The {sup 7}Be(p,{gamma}){sup 8}B and {sup 3}He({alpha},{gamma}){sup 7}Be reactions correspond to the most important uncertainties in solar model predictions of neutrino fluxes.

  4. WASP-8b: Characterization of a Cool and Eccentric Exoplanet with Spitzer

    NASA Astrophysics Data System (ADS)

    Cubillos, Patricio; Harrington, Joseph; Madhusudhan, Nikku; Stevenson, Kevin B.; Hardy, Ryan A.; Blecic, Jasmina; Anderson, David R.; Hardin, Matthew; Campo, Christopher J.

    2013-05-01

    WASP-8b has 2.18 times Jupiter's mass and is on an eccentric (e = 0.31) 8.16 day orbit. With a time-averaged equilibrium temperature of 948 K, it is one of the least-irradiated hot Jupiters observed with the Spitzer Space Telescope. We have analyzed six photometric light curves of WASP-8b during secondary eclipse observed in the 3.6, 4.5, and 8.0 μm Infrared Array Camera bands. The eclipse depths are 0.113% ± 0.018%, 0.069% ± 0.007%, and 0.093% ± 0.023%, respectively, giving respective brightness temperatures of 1552, 1131, and 938 K. We characterized the atmospheric thermal profile and composition of the planet using a line-by-line radiative transfer code and a Markov-chain Monte Carlo sampler. The data indicated no thermal inversion, independently of any assumption about chemical composition. We noted an anomalously high 3.6 μm brightness temperature (1552 K) by modeling the eccentricity-caused thermal variation, we found that this temperature is plausible for radiative timescales less than ~102 hr. However, as no model spectra fit all three data points well, the temperature discrepancy remains as an open question.

  5. Cast CF8C-Plus Stainless Steel for Turbocharger Applications

    SciTech Connect

    Maziasz, P.J.; Shyam, A.; Evans, N.D.; Pattabiraman, K. (Honeywell Turbo Technologies

    2010-06-30

    The purpose of this Cooperative Research and Development Agreement (CRADA) project is to provide the critical test data needed to qualify CF8C-Plus cast stainless steel for commercial production and use for turbocharger housings with upgraded performance and durability relative to standard commercial cast irons or stainless steels. The turbocharger technologies include, but are not limited to, heavy-duty highway diesel engines, and passenger vehicle diesel and gasoline engines. This CRADA provides additional critical high-temperature mechanical properties testing and data analysis needed to quality the new CF8C-Plus steels for turbocharger housing applications.

  6. The JT8D and JT9D engine component improvement: Performance improvement program

    NASA Technical Reports Server (NTRS)

    Gaffin, W. O.

    1982-01-01

    The NASA sponsored Engine Component Improvement - Performance Improvement Program at Pratt & Whitney Aircraft advanced the state of the art of thermal barrier coatings and ceramic seal systems, demonstrated the practicality of an advanced turbine clearance control system and an advanced fan design in the JT9D engine, and demonstrated the advantages of modern cooling, sealing, and aerodynamic designs in the high pressure turbine and compressor of the JT8D engine. Several of these improvements are already in airline service in JT8D and JT9D engines, and others will enter service soon in advanced models of these engines. In addition, the technology advances are being transferred to completely new engine configurations, the PW2037 engine and the NASA sponsored Energy Efficient Engine.

  7. Reduction of JT8D powered aircraft noise by engine refanning

    NASA Technical Reports Server (NTRS)

    Stitt, L. E.; Medeiros, A. A.

    1974-01-01

    The technical feasibility is described of substantially reducing the noise levels of existing JT8D powered aircraft by retrofitting the existing fleet with quieter refan engines and new acoustically treated nacelles. No major technical problems exist that preclude the development and installation of refanned engines on aircraft currently powered by the JT8D engine. The refan concept is technically feasible and provides calculated noise reductions of from 7 to 8 EPNdb for the B727-200 aircraft and from 10 to 12 EPNdb for the DC-9-32 aircraft at the FAR Part 36 measuring stations. These noise levels are lower than both the FAR Part 36 noise standards and the noise levels of the wide-body DC-10-10. Corresponding reductions in the 90 EPNdb footprint area are estimated to vary from about 70 percent for the DC-9 to about 80 percent for the B727.

  8. Reduction of JT8D powered aircraft noise by engine refanning

    NASA Technical Reports Server (NTRS)

    Stitt, L. E.; Medeiros, A. A.

    1974-01-01

    The purpose of the Refan Program is to establish the technical feasibility of substantially reducing the noise levels of existing JT8D powered aircraft. This would be accomplished by retrofitting the existing fleet with quieter refan engines and new acoustically treated nacelles. No major technical problems exist that preclude the development and installation of refanned engines on aircraft currently powered by the JT8D engine. The refan concept is technically feasible and provides calculated noise reductions of from 7 to 8 EPNdB for the B727-200 aircraft and from 10 to 12 EPNdB for the DC-9-32 aircraft at the FAR Part 36 measuring stations. Corresponding reductions in the 90 EPNdB footprint area are estimated to vary from about 70 percent for the DC-9 to about 80 percent for the B727.

  9. Flight test and numerical simulation of transonic flow around YAV-8B Harrier II wing

    NASA Technical Reports Server (NTRS)

    Gea, Lie-Mine; Chyu, Wei J.; Stortz, Michael W.; Roberts, Andrew C.; Chow, Chuen-Yen

    1991-01-01

    A computational fluid dynamics (CFD) method is used to study the aerodynamics of the YAV-8B Harrier II wing in the transonic region. A numerical procedure is developed to compute the flow field around the complicated wing-pylon-fairing geometry. The surface definition of the wing and pylons were obtained from direct measurement using theodolite triangulation. A thin-layer Navier-Stokes code with the Chimera technique is used to compute flow solutions. The computed pressure distributions at several span stations are compared with flight test data and show good agreement. Computed results are correlated with flight test data that show the flow is severely separated in the vicinity of the wing-pylon junction. Analysis shows that shock waves are induced by pylon swaybrace fairings, that the flow separation is much stronger at the outboard pylon and that the separation is caused mainly by the crossflow passing the geometry of wing-pylon junction.

  10. Searches for high frequency variations in the 8-B neutrino flux at the Sudbury neutrino observatory

    SciTech Connect

    Rielage, Keith; Seibert, Stanley R; Hime, Andrew; Elliott, Steven R; Stonehill, L C; Wouters, J M; Aharmim, B; Ahmed, S N; Anthony, A E; Barros, N; Beier, E W; Bellerive, A; Belttran, B; Bergevin, M; Biller, S D; Boudjemline, K; Burritt, T H; Cai, B; Chan, Y D; Chauhan, D; Chen, M; Cleveland, B T; Cox - Mobrand, G A; Dai, X; Deng, H; Detwiler, J; Dimarco, M; Doe, P J; Drouin, P - L; Duba, C A; Duncan, F A; Dunford, M; Earle, E D; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Ford, R J; Formaggilo, J A; Gagnon, N; Goon, J Tm; Guillian, E; Habib, S; Hahn, R L; Hallin, A L; Hallman, E D; Harvey, P J; Hazama, R; Heintzelman, W J; Heise, J; Helmer, R L; Howard, C; Howe, M A; Huang, M; Jamieson, B; Jelley, N A; Keeter, K J; Klein, J R; Kos, M; Kraus, C; Krauss, C B; Kutter, T; Kyba, C C M; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Loach, J C; Maclellan, R; Majerus, S; Mak, H B; Maneira, J; Martin, R; Mccauley, N; Mc Donald, A B; Mcgee, S; Miffin, C; Miller, M L; Monreal, B; Monroe, J; Morissette, B; Nickel, B G; Noble, A J; O' Keeffe, H M; Oblath, N S; Orebi Gann, G D; Oser, S M; Ott, R A; Peeters, S J M; Poon, A W P; Prior, G; Reitzner, S D; Robertson, B C; Robertson, R G H; Rollin, E; Schwendener, M H; Secrest, J A; Seibert, S R; Simard, O; Sinclair, D; Sinclair, L; Skensved, P; Sonley, T J; Tesic, G; Tolich, N; Tsui, T; Tunnell, C D; Van Berg, R; Van Devender, B A; Virtue, C J; Wall, B L; Waller, D; Wan Chan Tseung, H; West, N; Wilkerson, J F; Wilson, J R; Wright, A; Yeh, M; Zhang, F; Zuber, K

    2009-01-01

    We have peformed three searches for high-frequency signals in the solar neutrino flux measured by the Sudbury Neutrino Observatory (SNO), motivated by the possibility that solar g-mode oscillations could affect the production or propagation of solar {sup 8}B neutrinos. The first search looked for any significant peak in the frequency range l/day to 144/day, with a sensitivity to sinusoidal signals with amplitudes of 12% or greater. The second search focused on regions in which g-mode signals have been claimed by experiments aboard the SoHO satellite, and was sensitive to signals with amplitudes of 10% or greater. The third search looked for extra power across the entire frequency band. No statistically significant signal was detected in any of the three searches.

  11. 1995 Bird survey Foothills parkway section 8B National Park Service, Tennessee

    SciTech Connect

    Wade, M.C.; Giffen, N.R.; Wade, B.A.

    1996-05-01

    The Foothills Parkway Section 8B right-of-way (ROW) is a stretch of land between Pittman Center and Cosby, Tennessee that is approximately 14.2 miles long and 1,000 ft wide, with a considerably wider section on Webb Mountain. A breeding bird survey was conducted at selected sample points along the ROW. The intent of the survey was to identify bird communities, area sensitive species (birds dependent on extensive forest systems for all their needs) and endangered, threatened, federal candidate, and state `in need of management` species now using the ROW. The survey also provides baseline data to assess future habitat impacts as well as cumulative impacts of the project.

  12. WASP-8b: a retrograde transiting planet in a multiple system

    NASA Astrophysics Data System (ADS)

    Queloz, D.; Anderson, D. R.; Collier Cameron, A.; Gillon, M.; Hebb, L.; Hellier, C.; Maxted, P.; Pepe, F.; Pollacco, D.; Ségransan, D.; Smalley, B.; Triaud, A. H. M. J.; Udry, S.; West, R.

    2010-07-01

    We report the discovery of WASP-8b, a transiting planet of 2.25 ± 0.08 MJup on a strongly inclined eccentric 8.15-day orbit, moving in a retrograde direction to the rotation of its late-G host star. Evidence is found that the star is in a multiple stellar system with two other companions. The dynamical complexity of the system indicates that it may have experienced secular interactions such as the Kozai mechanism or a formation that differs from the “classical” disc-migration theory. Based on observations made with HARPS spectrograph on the 3.6-m ESO telescope and the EULER Swiss telescope at La Silla Observatory, Chile.Radial velocity data are only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/cgi-bin/qcat?J/A+A/517/L1

  13. The Coulomb Dissociation of {sup 8}B: A New Tool in Nuclear Astrophysics

    SciTech Connect

    Gai, Moshe

    2008-01-24

    The GSI1, GSI2 (as well as the RIKEN2 and the corrected GSI2) measurements of the Coulomb Dissociation (CD) of {sup 8}B are in good agreement with the most recent Direct Capture (DC) {sup 7}Be(p,{gamma}){sup 8}B reaction measurement performed at Weizmann and in agreement with the Seattle result. Yet it was claimed that the CD and DC results are sufficiently different and need to be reconciled. We show that these statements arise from a misunderstanding (as well as misrepresentation) of CD experiments. We recall a similar strong statement questioning the validity of the CD method due to an invoked large E2 component that was also shown to arise from a misunderstanding of the CD method. In spite of the good agreement between DC and CD data the slope of the astrophysical cross section factor (S{sub 17}) can not be extracted with high accuracy due to discrepancies among the most recent DC data as well as a discrepancies among the three reports of the GSI CD data. The slope is directly related to the d-wave component that dominates at higher energies. This d-wave component must be subtracted from measured data to extrapolate to zero energy. Hence the uncertainty of the measured slope leads to an additional downward uncertainty ({sub -3.0}{sup +0.0} eV-b) of the extrapolated zero energy cross section factor, S{sub 17}(0). Such an uncertainty is also consistent with the smaller value of S{sub 17}(0) extracted using the ANC method. This uncertainty must be alleviated by future experiments to allow a precise determination of S{sub 17}(0), a goal that so far has not be achieved in spite of strong statement(s) that appeared in the literature.

  14. Structure and stoichiometry of an accessory subunit TRIP8b interaction with hyperpolarization-activated cyclic nucleotide-gated channels

    PubMed Central

    Bankston, John R.; Camp, Stacey S.; DiMaio, Frank; Lewis, Alan S.; Chetkovich, Dane M.; Zagotta, William N.

    2012-01-01

    Ion channels operate in intact tissues as part of large macromolecular complexes that can include cytoskeletal proteins, scaffolding proteins, signaling molecules, and a litany of other molecules. The proteins that make up these complexes can influence the trafficking, localization, and biophysical properties of the channel. TRIP8b (tetratricopetide repeat-containing Rab8b-interacting protein) is a recently discovered accessory subunit of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels that contributes to the substantial dendritic localization of HCN channels in many types of neurons. TRIP8b interacts with the carboxyl-terminal region of HCN channels and regulates their cell-surface expression level and cyclic nucleotide dependence. Here we examine the molecular determinants of TRIP8b binding to HCN2 channels. Using a single-molecule fluorescence bleaching method, we found that TRIP8b and HCN2 form an obligate 4:4 complex in intact channels. Fluorescence-detection size-exclusion chromatography and fluorescence anisotropy allowed us to confirm that two different domains in the carboxyl-terminal portion of TRIP8b—the tetratricopepide repeat region and the TRIP8b conserved region—interact with two different regions of the HCN carboxyl-terminal region: the carboxyl-terminal three amino acids (SNL) and the cyclic nucleotide-binding domain, respectively. And finally, using X-ray crystallography, we determined the atomic structure of the tetratricopepide region of TRIP8b in complex with a peptide of the carboxy-terminus of HCN2. Together, these experiments begin to uncover the mechanism for TRIP8b binding and regulation of HCN channels. PMID:22550182

  15. JT8D-15/17 High Pressure Turbine Root Discharged Blade Performance Improvement. [engine design

    NASA Technical Reports Server (NTRS)

    Janus, A. S.

    1981-01-01

    The JT8D high pressure turbine blade and seal were modified, using a more efficient blade cooling system, improved airfoil aerodynamics, more effective control of secondary flows, and improved blade tip sealing. Engine testing was conducted to determine the effect of these improvements on performance. The modified turbine package demonstrated significant thrust specific fuel consumption and exhaust gas temperature improvements in sea level and altitude engine tests. Inspection of the improved blade and seal hardware after testing revealed no unusual wear or degradation.

  16. JT8D and JT9D jet engine performance improvement program. Task 1: Feasibility analysis

    NASA Technical Reports Server (NTRS)

    Gaffin, W. O.; Webb, D. E.

    1979-01-01

    JT8D and JT9D component performance improvement concepts which have a high probability of incorporation into production engines were identified and ranked. An evaluation method based on airline payback period was developed for the purpose of identifying the most promising concepts. The method used available test data and analytical models along with conceptual/preliminary designs to predict the performance improvements, weight, installation characteristics, cost for new production and retrofit, maintenance cost, and qualitative characteristics of candidate concepts. These results were used to arrive at the concept payback period, which is the time required for an airline to recover the investment cost of concept implementation.

  17. Aerodynamic performance of 0.4066-scale model to JT8D refan stage

    NASA Technical Reports Server (NTRS)

    Moore, R. D.; Kovich, G.; Tysl, E. R.

    1976-01-01

    The aerodynamic performance of a scale model of the split flow JT8D rafan stage is presented over a range of flows at speeds from 40 to 100 percent design. The bypass stage peak efficiency of 0.800 occurred at a total weight flow of 35.82 kilograms per second and a pressure ratio of 1.697. The stall margin was 15 percent based on pressure ratio and weight flow at stall and peak efficiency conditions. The data indicated that the hub region of the core stators was choked at design speed over the entire flow range tested.

  18. NASA refan program status. [for noise reduction of JT8D turbofan engine

    NASA Technical Reports Server (NTRS)

    Abdalla, K. L.; Yuska, J. A.

    1975-01-01

    The objective of the refan program is to demonstrate the technical feasibility of substantially reducing the noise levels of existing JT8D powered aircraft. The program consists of the design, manufacturing and testing of the refan engines and modified nacelles and airplanes. Experimental testing was completed for the refan engine both at sea level and at altitude conditions. Ground testing for the B727 side- and center-engine installations and flight testing of the DC-9 with refan engines and acoustic nacelles were performed. Preliminary results presented show that substantial noise reductions were achieved.

  19. Performance of aluminide coatings applied on alloy CF8C plus at 800 C

    SciTech Connect

    Kumar, Deepak; Dryepondt, Sebastien N; Shyam, Amit; Haynes, James A; Pint, Bruce A; Armstrong, Beth L; Lara-Curzio, Edgar

    2011-01-01

    The cost effective, austenitic stainless steel CF8C plus is an attractive alloy for massive cast structures such as steam turbine casings. The microstructure stability and creep strength of this alloy are better than commercial high-performance heat-resistant steels such as NF709 and Super 304H, and are comparable to the Ni-based superalloy Inconel 617. The oxidation resistance of the alloy in atmosphere rich in water vapor is however insufficient at T>800 C, and the use of diffusion aluminide coatings is considered for potential high temperature applications. The thermal stability and protectiveness of coatings applied on the CF8C plus substrate by pack cementation and slurry process were investigated in air + 10% H2O environment at 800 C. Further, the coating effect on the fatigue life of the alloy was assessed via low-cycle-fatigue experiments.

  20. Lateral aerodynamic parameters extracted from flight data for the F-8C airplane in maneuvering flight

    NASA Technical Reports Server (NTRS)

    Suit, W. T.

    1977-01-01

    Flight test data are used to extract the lateral aerodynamic parameters of the F-8C airplane at moderate to high angles of attack. The data were obtained during perturbations of the airplane from steady turns with trim normal accelerations from 1.5g to 3.0g. The angle-of-attack variation from trim was negligible. The aerodynamic coefficients extracted from flight data were compared with several other sets of coefficients, and the extracted coefficients resulted in characteristics for the Dutch roll mode (at the highest angles of attack) similar to those of a set of coefficients that have been the basis of several simulations of the F-8C.

  1. Wibault two-seat monoplane 8C2 : an all-metal pursuit and observation airplane

    NASA Technical Reports Server (NTRS)

    Serryer, J

    1926-01-01

    Michel Wibault's two seat monoplane 8C2, is similar to the Parasol pursuit monoplane which preceded it. It has no perishable parts in its structure and needs no special storage or coverings. The sample aeroplane uses a 500 HP Hispano-Suiza engine but can accept a 400-600HP engine from a variety of manufacturers with little difficulty. It uses a two blade tractor propeller.

  2. Toward the Total Synthesis of Amphidinolide N: Synthesis of the C8-C29 Fragment.

    PubMed

    Kawashima, Yuki; Toyoshima, Atsushi; Fuwa, Haruhiko; Sasaki, Makoto

    2016-05-01

    A synthesis of the C8-C29 fragment of amphidinolide N, a potent cytotoxic macrolide isolated from the marine dinoflagellate Amphidinium sp., has been achieved. The key features of the synthesis involve a convergent union of the C9-C15 and C16-C29 fragments by Steglich esterification and the construction of a pyran unit through a Tebbe methylenation/ring-closing metathesis sequence. PMID:27116189

  3. Pressure distribution for the wing of the YAV-8B airplane; with and without pylons

    NASA Technical Reports Server (NTRS)

    Saltzman, Edwin J.; Delfrate, John H.; Sabsay, Catherine M.; Yarger, Jill M.

    1992-01-01

    Pressure distribution data have been obtained in flight at four span stations on the wing panel of the YAV-8B airplane. Data obtained for the supercritical profiled wing, with and without pylons installed, ranged from Mach 0.46 to 0.88. The altitude ranged from approximately 20,000 to 40,000 ft and the resultant Reynolds numbers varied from approximately 7.2 million to 28.7 million based on the mean aerodynamic chord. Pressure distribution data and flow visualization results show that the full-scale flight wing performance is compromised because the lower surface cusp region experiences flow separation for some important transonic flight conditions. This condition is aggravated when local shocks occur on the lower surface of the wing (mostly between 20 and 35 percent chord) when the pylons are installed for Mach 0.8 and above. There is evidence that convex fairings, which cover the pylon attachment flanges, cause these local shocks. Pressure coefficients significantly more negative than those for sonic flow also occur farther aft on the lower surface (near 60 percent chord) whether or not the pylons are installed for Mach numbers greater than or equal to 0.8. These negative pressure coefficient peaks and associated local shocks would be expected to cause increasing wave and separation drag at transonic Mach number increases.

  4. DC-9 Flight Demonstration Program with Refanned JT8D Engines. Volume 3; Performance and Analysis

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The JT8D-109 engine has a sea level static, standard day bare engine takeoff thrust of 73,840 N. At sea level standard day conditions the additional thrust of the JT8D-109 results in 2,040 kg additional takeoff gross weight capability for a given field length. Range loss of the DC-9 Refan airplane for long range cruise was determined. The Refan airplane demonstrated stall, static longitudinal stability, longitudinal control, longitudinal trim, minimum control speeds, and directional control characteristics similar to the DC-9-30 production airplane and complied with airworthiness requirements. Cruise, climb, and thrust reverser performance were evaluated. Structural and dynamic ground test, flight test and analytical results substantiate Refan Program requirements that the nacelle, thrust reverser hardware, and the airplane structural modifications are flightworthy and certifiable and that the airplane meets flutter speed margins. Estimated unit cost of a DC-9 Refan retrofit program is 1.338 million in mid-1975 dollars with about an equal split in cost between airframe and engine.

  5. DC-9 flight demonstration program with refanned JT8D engines. Volume 4: Flyover noise

    NASA Technical Reports Server (NTRS)

    1975-01-01

    Flyover noise tests were conducted to determine the noise reductions achievable by modifying the engines and nacelles of DC-9-30 airplanes. The two stage fan of the JT8D-9 engine was replaced with a larger diameter, single stage fan and sound absorbing materials were incorporated in the engines and nacelles. The noise levels were determined to be 95.3 EPNdB at the sideline, 96.2 EPNdB for a full thrust takeoff, 87.5 EPNdB for takeoff with thrust cutback, and 97.4 EPNdB for landing approach. The noise reductions relative to the hardwall JT8D-9 were 8.2 EPNdB for takeoff with cutback and 8.7 EPNdB for landing. The 90 EPNdB noise contour areas were reduced by 40% for missions requiring maximum design takeoff and landing weights. For typical mission weights, the reductions were 19% for full thrust takeoff and 34% for takeoff with cutback. The 95 EPNdB contour areas were reduced by 50% for takeoff and 30% for takeoff with cutback for both missions.

  6. 7Be(p,(gamma))8B S-factor from Ab Initio No-Core Shell Model Wave Functions

    SciTech Connect

    Navratil, P; Bertulani, C A; Caurier, E

    2005-12-02

    Nuclear structure of {sup 7}Be, {sup 8}B and {sup 7,8}Li is studied within the ab initio no-core shell model (NCSM). Starting from high-precision nucleon-nucleon (NN) interactions, wave functions of {sup 7}Be and {sup 8}B bound states are obtained in basis spaces up to 10 h bar{Omega} and used to calculate channel cluster form factors (overlap integrals) of the {sup 8}B ground state with {sup 7}Be+p. Due to the use of the harmonic oscillator (HO) basis, the overlap integrals have incorrect asymptotic properties. We fix this problem in two alternative ways. First, by a Woods-Saxon (WS) potential solution fit to the interior of the NCSM overlap integrals. Second, by a direct matching with the Whittaker function. The corrected overlap integrals are then used for the {sup 7}Be(p,{gamma}){sup 8}B S-factor calculation. We study the convergence of the S-factor with respect to the NCSM HO frequency and the model space size. Our S-factor results are in agreement with recent direct measurement data. We also test the spectroscopic factors and the corrected overlap integrals from the NCSM in describing the momentum distributions in knockout reactions with {sup 8}B projectiles. A good agreement with the available experimental data is also found, attesting the overall consistency of the calculations.

  7. Evidence for a functional subdivision of Premotor Ear-Eye Field (Area 8B)

    PubMed Central

    Lanzilotto, Marco; Perciavalle, Vincenzo; Lucchetti, Cristina

    2015-01-01

    The Supplementary Eye Field (SEF) and the Frontal Eye Field (FEF) have been described as participating in gaze shift control. Recent evidence suggests, however, that other areas of the dorsomedial prefrontal cortex also influence gaze shift. Herein, we have investigated electrically evoked ear- and eye movements from the Premotor Ear-Eye Field, or PEEF (area 8B) of macaque monkeys. We stimulated PEEF during spontaneous condition (outside the task performance) and during the execution of a visual fixation task (VFT). In the first case, we functionally identified two regions within the PEEF: a core and a belt. In the core region, stimulation elicited forward ear movements; regarding the evoked eye movements, in some penetrations, stimulation elicited contraversive fixed-vectors with a mean amplitude of 5.14°; while in other penetrations, we observed prevalently contralateral goal-directed eye movements having end-points that fell within 15° in respect to the primary eye position. On the contrary, in the belt region, stimulation elicited backward ear movements; regarding the eye movements, in some penetrations stimulation elicited prevalently contralateral goal-directed eye movements having end-points that fell within 15° in respect to the primary eye position, while in the lateral edge of the investigated region, stimulation elicited contralateral goal-directed eye movements having end-points that fell beyond 15° in respect to the primary eye position. Stimulation during VFT either did not elicit eye movements or evoked saccades of only a few degrees. Finally, even though no head rotation movements were observed during the stimulation period, we viewed a relationship between the duration of stimulation and the neck forces exerted by the monkey's head. We propose an updated vision of the PEEF composed of two functional regions, core and belt, which may be involved in integrating auditory and visual information important to the programming of gaze orienting

  8. DISCOVERY AND ROSSITER-McLAUGHLIN EFFECT OF EXOPLANET KEPLER-8b

    SciTech Connect

    Jenkins, Jon M.; Caldwell, Douglas A.; Marcy, Geoffrey W.; Basri, Gibor; Isaacson, Howard; Cochran, William D.; Endl, Michael; Welsh, William F.; Batalha, Natalie M.; Buchhave, Lars A.; Geary, John C.; Brown, Timothy M.; Dunham, Edward W.; Fischer, Debra A.; Gautier, Thomas N.; Howell, Steve B.; Johnson, John Asher

    2010-12-01

    We report on the discovery and the Rossiter-McLaughlin (R-M) effect of Kepler-8b, a transiting planet identified by the NASA Kepler Mission. Kepler photometry and Keck-HIRES radial velocities yield the radius and mass of the planet around this F8IV subgiant host star. The planet has a radius R{sub P} = 1.419 R{sub J} and a mass M{sub P} = 0.60 M{sub J}, yielding a density of 0.26 g cm{sup -3}, one of the lowest planetary densities known. The orbital period is P = 3.523 days and the orbital semimajor axis is 0.0483{sup +0.0006}{sub -0.0012} AU. The star has a large rotational vsin i of 10.5 {+-} 0.7 km s{sup -1} and is relatively faint (V {approx} 13.89 mag); both properties are deleterious to precise Doppler measurements. The velocities are indeed noisy, with scatter of 30 m s{sup -1}, but exhibit a period and phase that are consistent with those implied by transit photometry. We securely detect the R-M effect, confirming the planet's existence and establishing its orbit as prograde. We measure an inclination between the projected planetary orbital axis and the projected stellar rotation axis of {lambda} = -26.{sup 0}4 {+-} 10.{sup 0}1, indicating a significant inclination of the planetary orbit. R-M measurements of a large sample of transiting planets from Kepler will provide a statistically robust measure of the true distribution of spin-orbit orientations for hot Jupiters around F and early G stars.

  9. Discovery and Rossiter-Mclaughlin Effect of Exoplanet Kepler-8b

    NASA Astrophysics Data System (ADS)

    Jenkins, Jon M.; Borucki, William J.; Koch, David G.; Marcy, Geoffrey W.; Cochran, William D.; Welsh, William F.; Basri, Gibor; Batalha, Natalie M.; Buchhave, Lars A.; Brown, Timothy M.; Caldwell, Douglas A.; Dunham, Edward W.; Endl, Michael; Fischer, Debra A.; Gautier, Thomas N., III; Geary, John C.; Gilliland, Ronald L.; Howell, Steve B.; Isaacson, Howard; Johnson, John Asher; Latham, David W.; Lissauer, Jack J.; Monet, David G.; Rowe, Jason F.; Sasselov, Dimitar D.; Howard, Andrew W.; MacQueen, Phillip; Orosz, Jerome A.; Chandrasekaran, Hema; Twicken, Joseph D.; Bryson, Stephen T.; Quintana, Elisa V.; Clarke, Bruce D.; Li, Jie; Allen, Christopher; Tenenbaum, Peter; Wu, Hayley; Meibom, Søren; Klaus, Todd C.; Middour, Christopher K.; Cote, Miles T.; McCauliff, Sean; Girouard, Forrest R.; Gunter, Jay P.; Wohler, Bill; Hall, Jennifer R.; Ibrahim, Khadeejah; Kamal Uddin, AKM; Wu, Michael S.; Bhavsar, Paresh A.; Van Cleve, Jeffrey; Pletcher, David L.; Dotson, Jessie L.; Haas, Michael R.

    2010-12-01

    We report on the discovery and the Rossiter-McLaughlin (R-M) effect of Kepler-8b, a transiting planet identified by the NASA Kepler Mission. Kepler photometry and Keck-HIRES radial velocities yield the radius and mass of the planet around this F8IV subgiant host star. The planet has a radius R P = 1.419 R J and a mass M P = 0.60 M J, yielding a density of 0.26 g cm-3, one of the lowest planetary densities known. The orbital period is P = 3.523 days and the orbital semimajor axis is 0.0483+0.0006 -0.0012 AU. The star has a large rotational vsin i of 10.5 ± 0.7 km s-1 and is relatively faint (V ≈ 13.89 mag); both properties are deleterious to precise Doppler measurements. The velocities are indeed noisy, with scatter of 30 m s-1, but exhibit a period and phase that are consistent with those implied by transit photometry. We securely detect the R-M effect, confirming the planet's existence and establishing its orbit as prograde. We measure an inclination between the projected planetary orbital axis and the projected stellar rotation axis of λ = -26fdg4 ± 10fdg1, indicating a significant inclination of the planetary orbit. R-M measurements of a large sample of transiting planets from Kepler will provide a statistically robust measure of the true distribution of spin-orbit orientations for hot Jupiters around F and early G stars. Based in part on observations obtained at the W. M. Keck Observatory, which is operated as a scientific partnership between the California Institute of Technology, the University of California, and the National Aeronautics and Space Administration. The Observatory was made possible by the generous financial support of the W. M. Keck Foundation.

  10. Engine component improvement: JT8D and JT9D performance improvements

    NASA Technical Reports Server (NTRS)

    Gaffin, W. O.

    1978-01-01

    A feasibility analysis screening method for predicting the airline acceptance of a proposed engine performance improvement modification was developed. Technical information derived from available test data and analytical models is used along with conceptual/preliminary designs to establish the predicted performance improvement, weight and installation characteristics, the cost for new production and retrofit, maintenance cost and qualitative characteristics of the performance improvement concepts being evaluated. These results are used to arrive at the payback period, which is the time required for an airline to recover the investment cost of concept implementation, and to predict the amount of fuel saved by a performance improvement concept. The assumptions used to calculate the payback period and fuel saved are discussed. A summary of the results when the screening method is applied is presented for several representative JT8D and JT9D performance improvement concepts. An example of the input information used to develop the summary results is shown.

  11. Chamigrane Sesquiterpenes from a Basidiomycetous Endophytic Fungus XG8D Associated with Thai Mangrove Xylocarpus granatum.

    PubMed

    Choodej, Siwattra; Teerawatananond, Thapong; Mitsunaga, Tohru; Pudhom, Khanitha

    2016-01-01

    Six new chamigrane sesquiterpenes, merulinols A‒F (1‒6), and four known metabolites (7‒10) were isolated from the culture of the basidiomycetous fungus XG8D, a mangrove-derived endophyte. Their structures were elucidated mainly by 1D and 2D NMR, while the structures of 1 and 2 were further confirmed by single-crystal X-ray diffraction analysis. The in vitro cytotoxicity of all compounds was evaluated against three human cancer cell lines, MCF-7, Hep-G2, and KATO-3. Compounds 3 and 4 selectively displayed cytotoxicity against KATO-3 cells with IC50 values of 35.0 and 25.3 μM, respectively. PMID:27428984

  12. The 727 airplane target thrust reverser static performance model test for refanned JT8D engines

    NASA Technical Reports Server (NTRS)

    Chow, C. T. P.; Atkey, E. N.

    1974-01-01

    The results of a scale model static performance test of target thrust reverser configurations for the Pratt and Whitney Aircraft JT8D-100 series engine are presented. The objective of the test was to select a series of suitable candidate reverser configurations for the subsequent airplane model wind tunnel ingestion and flight controls tests. Test results indicate that adequate reverse thrust performance with compatible engine airflow match is achievable for the selected configurations. Tapering of the lips results in loss of performance and only minimal flow directivity. Door pressure surveys were conducted on a selected number of lip and fence configurations to obtain data to support the design of the thrust reverser system.

  13. JT8D revised high-pressure turbine cooling and other outer air seal program

    NASA Technical Reports Server (NTRS)

    Gaffin, W. O.

    1979-01-01

    The JT8D high pressure turbine was revised to reduce leakage between the blade tip shrouds and the outer air seal, and engine testing was performed to determine the effect on performance. The addition of a second knife-edge on the blade tip shroud, the extension of the honeycomb seal land to cover the added knife-edge and an existing spoiler on the shroud, and a material substitution in the seal support ring to improve thermal growth characteristics are included. A relocation of the blade cooling air discharge to insure adequate cooling flow is required. Significant specific fuel consumption and exhaust gas temperature improvements were demonstrated with the revised turbine in sea level and simulated altitude engine tests. Inspection of the revised seal hardware after these tests showed no unusual wear or degradation.

  14. Chamigrane Sesquiterpenes from a Basidiomycetous Endophytic Fungus XG8D Associated with Thai Mangrove Xylocarpus granatum

    PubMed Central

    Choodej, Siwattra; Teerawatananond, Thapong; Mitsunaga, Tohru; Pudhom, Khanitha

    2016-01-01

    Six new chamigrane sesquiterpenes, merulinols A‒F (1‒6), and four known metabolites (7‒10) were isolated from the culture of the basidiomycetous fungus XG8D, a mangrove-derived endophyte. Their structures were elucidated mainly by 1D and 2D NMR, while the structures of 1 and 2 were further confirmed by single-crystal X-ray diffraction analysis. The in vitro cytotoxicity of all compounds was evaluated against three human cancer cell lines, MCF-7, Hep-G2, and KATO-3. Compounds 3 and 4 selectively displayed cytotoxicity against KATO-3 cells with IC50 values of 35.0 and 25.3 μM, respectively. PMID:27428984

  15. Sc2@C3v(8)-C82 vs. Sc2C2@C3v(8)-C82: drastic effect of C2 capture on the redox properties of scandium metallofullerenes.

    PubMed

    Kurihara, Hiroki; Lu, Xing; Iiduka, Yuko; Mizorogi, Naomi; Slanina, Zdenek; Tsuchiya, Takahiro; Nagase, Shigeru; Akasaka, Takeshi

    2012-01-30

    We describe the first example of scandium dimetallofullerenes, Sc(2)@C(3v)(8)-C(82), which has the same cage as the previously assigned scandium carbide cluster fullerene Sc(2)C(2)@C(3v)(8)-C(82) but they exhibit distinctly different electronic configurations and electronic behaviours, confirming the drastic influence of the internal C(2) unit.

  16. 17 CFR 239.16 - Form S-6, for unit investment trusts registered on Form N-8B-2.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Form S-6, for unit investment... § 239.16 Form S-6, for unit investment trusts registered on Form N-8B-2. This form may be used for registration under the Securities Act of 1933 of securities of any unit investment trust registered under...

  17. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... under the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8B-4, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... SECURITIES AND EXCHANGE COMMISSION (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF...

  18. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... under the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8B-4, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... SECURITIES AND EXCHANGE COMMISSION (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF...

  19. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... under the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8B-4, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... SECURITIES AND EXCHANGE COMMISSION (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF...

  20. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... under the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8B-4, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... SECURITIES AND EXCHANGE COMMISSION (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF...

  1. 17 CFR 274.14 - Form N-8B-4, registration statements of face-amount certificate companies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... under the Investment Company Act of 1940. Editorial Note: For Federal Register citations affecting Form N-8B-4, see the List of CFR Sections Affected, which appears in the Finding Aids section of the... SECURITIES AND EXCHANGE COMMISSION (CONTINUED) FORMS PRESCRIBED UNDER THE INVESTMENT COMPANY ACT OF...

  2. SEARCH FOR GLOBAL f-MODES AND p-MODES IN THE {sup 8}B NEUTRINO FLUX

    SciTech Connect

    Lopes, Ilídio E-mail: ilopes@uevora.pt

    2013-11-01

    The impact of global acoustic modes on the {sup 8}B neutrino flux time series is computed for the first time. It is shown that the time fluctuations of the {sup 8}B neutrino flux depend on the amplitude of acoustic eigenfunctions in the region where the {sup 8}B neutrino flux is produced: modes with low n (or order) that have eigenfunctions with a relatively large amplitude in the Sun's core strongly affect the neutrino flux; conversely, modes with high n that have eigenfunctions with a minimal amplitude in the Sun's core have a very small impact on the neutrino flux. It was found that the global modes with a larger impact on the {sup 8}B neutrino flux have a frequency of oscillation in the interval 250 μHz to 500 μHz (or a period in the interval 30 minutes to 70 minutes), such as the f-modes (n = 0) for the low degrees, radial modes of order n ≤ 3, and the dipole mode of order n = 1. Their corresponding neutrino eigenfunctions are very sensitive to the solar inner core and are unaffected by the variability of the external layers of the solar surface. If time variability of neutrinos is observed for these modes, it will lead to new ways of improving the sound speed profile inversion in the central region of the Sun.

  3. A Functional Link between AMPK and Orexin Mediates the Effect of BMP8B on Energy Balance.

    PubMed

    Martins, Luís; Seoane-Collazo, Patricia; Contreras, Cristina; González-García, Ismael; Martínez-Sánchez, Noelia; González, Francisco; Zalvide, Juan; Gallego, Rosalía; Diéguez, Carlos; Nogueiras, Rubén; Tena-Sempere, Manuel; López, Miguel

    2016-08-23

    AMP-activated protein kinase (AMPK) in the ventromedial nucleus of the hypothalamus (VMH) and orexin (OX) in the lateral hypothalamic area (LHA) modulate brown adipose tissue (BAT) thermogenesis. However, whether these two molecular mechanisms act jointly or independently is unclear. Here, we show that the thermogenic effect of bone morphogenetic protein 8B (BMP8B) is mediated by the inhibition of AMPK in the VMH and the subsequent increase in OX signaling via the OX receptor 1 (OX1R). Accordingly, the thermogenic effect of BMP8B is totally absent in ox-null mice. BMP8B also induces browning of white adipose tissue (WAT), its thermogenic effect is sexually dimorphic (only observed in females), and its impact on OX expression and thermogenesis is abolished by the knockdown of glutamate vesicular transporter 2 (VGLUT2), implicating glutamatergic signaling. Overall, our data uncover a central network controlling energy homeostasis that may be of considerable relevance for obesity and metabolic disorders. PMID:27524625

  4. Hydrocarbons in the C8--C20 range measured during COAST study in Texas

    SciTech Connect

    Zielinska, B.; Sagebiel, J.; Sheetz, L.H.; Harshfield, G.; Uberna, E.; Hauze, W.J.; Price, J.H.

    1994-12-31

    Hydrocarbons in the C8--C20 range were collected using Tenax-TA solid adsorbent cartridges during the Coastal Oxidant Assessment for Southeast Texas (COAST) field study, carried out in the Houston-Galveston and Beaumont-Port Arthur areas. Ambient samples were collected at the Galleria, Clinton and Port Arthur sampling sites, from August 26 to August 30, 1993. In addition, a number of Tenax samples were collected in the vicinity of major industrial complexes (such as Exxon, AMOCO Oil, Union Carbide, Dow Chemical) and in an urban tunnel. All samples were analyzed with high resolution capillary column gas chromatography with flame ionization detection (GC/FID) for quantification and selected duplicate samples were analyzed with combined gas chromatography/Fourier transform infrared/mass spectrometry (GC/FTIR/MS) for identification of individual compounds. The results of these analyses are presented.

  5. Gaseous exhaust emissions from a JT8D-109 turbofan engine at simulated cruise flight conditions

    NASA Technical Reports Server (NTRS)

    Diehl, L. A.; Holdeman, J. D.

    1975-01-01

    Gaseous emissions from a JT8D-109 turbofan engine were measured in an altitude facility at four simulated cruise flight conditions: Mach 0.8 at altitudes of 9.1, 10, 7, and 12.2 km and Mach 0.9 at 10.7 km. Engine inlet air temperature was held constant at 283 K for all tests. Emissions measurements were made at nominally 6 cm intervals across the horizontal diameter of the engine exhaust nozzle with a single-point traversing gas sample probe. Measured emissions of decreased with increasing altitude from an emission index of 10.4 to one of 8.3, while carbon monoxide increased with increasing altitude from an emission index of 1.6 to one of 4.4. Unburned hydrocarbon emissions were essentially negligible for all flight conditions. Since the engine inlet air temperatures were not correctly simulated, the NOx emission indices were corrected to true altitude conditions by using correlating parameters for changes in combustor inlet temperature, pressure, and temperature rise. The correction was small at the lowest altitude. At the 10.7 and 12.2 km, Mach 0.8 test conditions the correction decreased the measured values by 1 emission index.

  6. Evidence for electron neutrino flavor change through measurement of the (8)B solar neutrino flux at the Sudbury Neutrino Observatory

    NASA Astrophysics Data System (ADS)

    Neubauer, Mark Stephen

    2001-11-01

    The Sudbury Neutrino Observatory (SNO) is a water Cerenkov detector designed to study solar neutrinos. Using 1 kiloton of heavy water as the target and detection medium, SNO is able to separately determine the flux of electron neutrinos (νe) and the flux of all active neutrinos from the Sun by measuring the rate of charged current (CC) and neutral current (NC) interactions with deuterons. A comparison of these interaction rates allows for direct observation of solar neutrino oscillations. SNO can also search for oscillations by comparing the rate of CC and neutrino- electron elastic scattering (ES) events, since ES has both charged current and neutral current sensitivity. In this thesis, we present measurement of the 8B solar ν e flux of 1.78+0.13-0.14 (stat+syst) × 106cm-2s -1 (35% BP2000 SSM) through measurement of the CC rate over 169.3 days of livetime. We have also measured the 8B flux from the ES reaction to be 2.56+0.48-0.45 (stat+syst), consistent with measurements by previous water Cerenkov experiments. A flavor analysis comparing the CC measured flux with that determined through ES by SuperKamiokande yields a non- νe active neutrino flux from 8B of 3.62+1.06-1.08 × 106cm-2s-1 , providing evidence for νe --> ν μ,τ oscillations as a solution to the solar neutrino problem. This result excludes pure solar νe --> ν s oscillations at greater than the 99.7% C.I. The total active 8B neutrino flux has been measured to be 5.39+1.07-1.09 × 106cm-2s-1 , consistent with BP2000 SSM predictions. First analyses of the CC (NHit) spectrum and hep flux in SNO are presented. The CC spectrum is found to be a good fit to expectations from an undistorted 8B spectrum, and global best fit vacuum oscillation solutions are disfavored over the other solutions by the data. Through observations near the 8B endpoint with consideration of energy systematics, hep flux limits of 4.1 (90% C.I.) and 6.9 (99% C.I.) times SSM expectations are obtained. A statistical fit for the

  7. Measurement of the {sup 8}B solar neutrino flux with the KamLAND liquid scintillator detector

    SciTech Connect

    Abe, S.; Furuno, K.; Gando, A.; Gando, Y.; Ichimura, K.; Ikeda, H.; Kibe, Y.; Kimura, W.; Kishimoto, Y.; Minekawa, Y.; Mitsui, T.; Morikawa, T.; Nagai, N.; Nakajima, K.; Nakamura, M.; Narita, K.; Shimizu, I.; Shimizu, Y.; Shirai, J.; Suekane, F.

    2011-09-15

    We report a measurement of the neutrino-electron elastic scattering rate from {sup 8}B solar neutrinos based on a 123 kton-day exposure of KamLAND. The background-subtracted electron recoil rate, above a 5.5-MeV analysis threshold is 1.49 {+-} 0.14(stat) {+-} 0.17(syst) events per kton-day. Interpreted as due to a pure electron flavor flux with a {sup 8}B neutrino spectrum, this corresponds to a spectrum integrated flux of 2.77 {+-} 0.26(stat) {+-} 0.32(syst) x10{sup 6} cm{sup -2}s{sup -1}. The analysis threshold is driven by {sup 208}Tl present in the liquid scintillator, and the main source of systematic uncertainty is due to background from cosmogenic {sup 11}Be. The measured rate is consistent with existing measurements and with standard solar model predictions which include matter-enhanced neutrino oscillation.

  8. Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea

    SciTech Connect

    Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; Sà, Elisabet Laia; Ignacio-Espinoza, J. Cesar; Cornejo-Castillo, Francisco M.; Verberkmoes, Nathan C.; Vaqué, Dolors; Sullivan, Matthew B.; Acinas, Silvia G.; Kellogg, Christina A.

    2015-01-14

    Marine viruses (phages) alter bacterial diversity and evolution with impacts on marine biogeochemical cycles, and yet few well-developed model systems limit opportunities for hypothesis testing. We isolate phage B8b from the Mediterranean Sea using Pseudoalteromonas sp. QC-44 as a host and characterize it using myriad techniques. Morphologically, phage B8b was classified as a member of the Siphoviridae family. One-step growth analyses showed that this siphovirus had a latent period of 70 min and released 172 new viral particles per cell. In the host range analysis against 89 bacterial host strains revealed that phage B8b infected 3 Pseudoalteromonas strains (52 tested, >99.9% 16S rRNA gene nucleotide identity) and 1 non-Pseudoaltermonas strain belonging to Alteromonas sp. (37 strains from 6 genera tested), which helps bound the phylogenetic distance possible in a phage-mediated horizontal gene transfer event. The Pseudoalteromonas phage B8b genome size was 42.7 kb, with clear structural and replication modules where the former were delineated leveraging identification of 16 structural genes by virion structural proteomics, only 4 of which had any similarity to known structural proteins. In nature, this phage was common in coastal marine environments in both photic and aphotic layers (found in 26.5% of available viral metagenomes), but not abundant in any sample (average per sample abundance was 0.65% of the reads). Together these data improve our understanding of siphoviruses in nature, and provide foundational information for a new 'rare virosphere' phage-host model system.

  9. Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea

    DOE PAGES

    Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; Sà, Elisabet Laia; Ignacio-Espinoza, J. Cesar; Cornejo-Castillo, Francisco M.; Verberkmoes, Nathan C.; Vaqué, Dolors; Sullivan, Matthew B.; Acinas, Silvia G.; et al

    2015-01-14

    Marine viruses (phages) alter bacterial diversity and evolution with impacts on marine biogeochemical cycles, and yet few well-developed model systems limit opportunities for hypothesis testing. We isolate phage B8b from the Mediterranean Sea using Pseudoalteromonas sp. QC-44 as a host and characterize it using myriad techniques. Morphologically, phage B8b was classified as a member of the Siphoviridae family. One-step growth analyses showed that this siphovirus had a latent period of 70 min and released 172 new viral particles per cell. In the host range analysis against 89 bacterial host strains revealed that phage B8b infected 3 Pseudoalteromonas strains (52 tested,more » >99.9% 16S rRNA gene nucleotide identity) and 1 non-Pseudoaltermonas strain belonging to Alteromonas sp. (37 strains from 6 genera tested), which helps bound the phylogenetic distance possible in a phage-mediated horizontal gene transfer event. The Pseudoalteromonas phage B8b genome size was 42.7 kb, with clear structural and replication modules where the former were delineated leveraging identification of 16 structural genes by virion structural proteomics, only 4 of which had any similarity to known structural proteins. In nature, this phage was common in coastal marine environments in both photic and aphotic layers (found in 26.5% of available viral metagenomes), but not abundant in any sample (average per sample abundance was 0.65% of the reads). Together these data improve our understanding of siphoviruses in nature, and provide foundational information for a new 'rare virosphere' phage-host model system.« less

  10. Life-style and genome structure of marine Pseudoalteromonas siphovirus B8b isolated from the northwestern Mediterranean Sea.

    PubMed

    Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; Sà, Elisabet Laia; Ignacio-Espinoza, J Cesar; Cornejo-Castillo, Francisco M; Verberkmoes, Nathan C; Vaqué, Dolors; Sullivan, Matthew B; Acinas, Silvia G

    2015-01-01

    Marine viruses (phages) alter bacterial diversity and evolution with impacts on marine biogeochemical cycles, and yet few well-developed model systems limit opportunities for hypothesis testing. Here we isolate phage B8b from the Mediterranean Sea using Pseudoalteromonas sp. QC-44 as a host and characterize it using myriad techniques. Morphologically, phage B8b was classified as a member of the Siphoviridae family. One-step growth analyses showed that this siphovirus had a latent period of 70 min and released 172 new viral particles per cell. Host range analysis against 89 bacterial host strains revealed that phage B8b infected 3 Pseudoalteromonas strains (52 tested, >99.9% 16S rRNA gene nucleotide identity) and 1 non-Pseudoaltermonas strain belonging to Alteromonas sp. (37 strains from 6 genera tested), which helps bound the phylogenetic distance possible in a phage-mediated horizontal gene transfer event. The Pseudoalteromonas phage B8b genome size was 42.7 kb, with clear structural and replication modules where the former were delineated leveraging identification of 16 structural genes by virion structural proteomics, only 4 of which had any similarity to known structural proteins. In nature, this phage was common in coastal marine environments in both photic and aphotic layers (found in 26.5% of available viral metagenomes), but not abundant in any sample (average per sample abundance was 0.65% of the reads). Together these data improve our understanding of siphoviruses in nature, and provide foundational information for a new 'rare virosphere' phage-host model system. PMID:25587991

  11. Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean Sea

    PubMed Central

    Lara, Elena; Holmfeldt, Karin; Solonenko, Natalie; Sà, Elisabet Laia; Ignacio-Espinoza, J. Cesar; Cornejo-Castillo, Francisco M.; Verberkmoes, Nathan C.; Vaqué, Dolors; Sullivan, Matthew B.; Acinas, Silvia G.

    2015-01-01

    Marine viruses (phages) alter bacterial diversity and evolution with impacts on marine biogeochemical cycles, and yet few well-developed model systems limit opportunities for hypothesis testing. Here we isolate phage B8b from the Mediterranean Sea using Pseudoalteromonas sp. QC-44 as a host and characterize it using myriad techniques. Morphologically, phage B8b was classified as a member of the Siphoviridae family. One-step growth analyses showed that this siphovirus had a latent period of 70 min and released 172 new viral particles per cell. Host range analysis against 89 bacterial host strains revealed that phage B8b infected 3 Pseudoalteromonas strains (52 tested, >99.9% 16S rRNA gene nucleotide identity) and 1 non-Pseudoaltermonas strain belonging to Alteromonas sp. (37 strains from 6 genera tested), which helps bound the phylogenetic distance possible in a phage-mediated horizontal gene transfer event. The Pseudoalteromonas phage B8b genome size was 42.7 kb, with clear structural and replication modules where the former were delineated leveraging identification of 16 structural genes by virion structural proteomics, only 4 of which had any similarity to known structural proteins. In nature, this phage was common in coastal marine environments in both photic and aphotic layers (found in 26.5% of available viral metagenomes), but not abundant in any sample (average per sample abundance was 0.65% of the reads). Together these data improve our understanding of siphoviruses in nature, and provide foundational information for a new ‘rare virosphere’ phage–host model system. PMID:25587991

  12. Range 8C Rehabilitation Demonstration Project, Hohenfels Training Area, Germany: Final report

    SciTech Connect

    Zellmer, S.D.; Hinchman, R.R.; Johnson, D.O.; Severinghaus, W.D.; Brent, J.J.

    1991-11-01

    More than 30 years of intensive and continual tactical training has caused extensive environmental damage at the US Army Hohenfels Training Area in Germany. The Range 8C Rehabilitation Demonstration Project, followed by a three-year monitoring effort, was conducted to develop and evaluate the environmental and economic effectiveness of seven revegetation and four erosion control prescriptions implemented at a 16-ha site. The point-intercept method was used to measure the types and amounts of vegetation established and the changes in the vegetative community during three years of military use on the seven areas treated with revegetation prescriptions. Field observations were made to determine the suitability and durability of four types of erosion control structures. Soil fertility and a source of seed appeared to be the most limiting factors in establishing vegetation, while seedbed preparation had only a minor influence. Grasses appeared to be more resistant to vehicle traffic than did other types of vegetation. Because grassed waterways were used as roads by military vehicles and a system of graded terraces was expensive, these erosion control prescriptions were unsuitable and uneconomical for use on training areas. Low-cost riprap waterbars and porous check dams slowed the velocity of runoff, trapped sediments, and were durable. Recommendations were formulated to improve the environmental and economic effectiveness of future rehabilitation efforts on tactical training areas.

  13. Improving a recombinant Zymomonas mobilis strain 8b through continuous adaptation on dilute acid pretreated corn stover hydrolysate

    DOE PAGES

    Mohagheghi, Ali; Linger, Jeffrey G.; Yang, Shihui; Smith, Holly; Dowe, Nancy; Zhang, Min; Pienkos, Philip T.

    2015-03-31

    Complete conversion of the major sugars of biomass including both the C5 and C6 sugars is critical for biofuel production processes. Several inhibitory compounds like acetate, hydroxymethylfurfural (HMF), and furfural are produced from the biomass pretreatment process leading to ‘hydrolysate toxicity,’ a major problem for microorganisms to achieve complete sugar utilization. Therefore, development of more robust microorganisms to utilize the sugars released from biomass under toxic environment is critical. In this study, we use continuous culture methodologies to evolve and adapt the ethanologenic bacterium Zymomonas mobilis to improve its ethanol productivity using corn stover hydrolysate. The results are the following:more » A turbidostat was used to adapt the Z. mobilis strain 8b in the pretreated corn stover liquor. The adaptation was initiated using pure sugar (glucose and xylose) followed by feeding neutralized liquor at different dilution rates. Once the turbidostat reached 60% liquor content, the cells began washing out and the adaptation was stopped. Several ‘sub-strains’ were isolated, and one of them, SS3 (sub-strain 3), had 59% higher xylose utilization than the parent strain 8b when evaluated on 55% neutralized PCS (pretreated corn stover) liquor. Using saccharified PCS slurry generated by enzymatic hydrolysis from 25% solids loading, SS3 generated an ethanol yield of 75.5% compared to 64% for parent strain 8b. Furthermore, the total xylose utilization was 57.7% for SS3 versus 27.4% for strain 8b. To determine the underlying genotypes in these new sub-strains, we conducted genomic resequencing and identified numerous single-nucleotide mutations (SNPs) that had arisen in SS3. We further performed quantitative reverse transcription PCR (qRT-PCR) on genes potentially affected by these SNPs and identified significant down-regulation of two genes, ZMO0153 and ZMO0776, in SS3 suggesting potential genetic mechanisms behind SS3’s improved

  14. Frequency measurement of the 2S--8S/8D transitions in atomic hydrogen: Determination of the Rydberg constant

    SciTech Connect

    Nez, F.; Plimmer, M.D.; Bourzeix, S.; Julien, L.; Biraben, F. ); Felder, R. )

    1993-06-10

    We have performed a new measurement of the Rydberg constant by frequency comparison of the 2S--8S and 2S--8D two-photon transitions in hydrogen with the difference of two optical standards connected to a frequency chain. Our result is: R[sub [infinity

  15. 13 CFR 121.411 - What are the size procedures for SBA's Section 8(d) Subcontracting Program?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What are the size procedures for SBA's Section 8(d) Subcontracting Program? 121.411 Section 121.411 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION SMALL BUSINESS SIZE REGULATIONS Size Eligibility Provisions and...

  16. [Clinical feature and ATP8B1 mutation analysis of a patient with progressive familial intrahepatic cholestasis type I].

    PubMed

    Cheng, Ying; Guo, Li; Song, Yuan-Zong

    2016-08-01

    Progressive familial intrahepatic cholestasis type I (PFIC1) is an autosomal recessive disorder caused by biallelic mutations of ATP8B1 gene, with progressive cholestasis as the main clinical manifestation. This paper reports the clinical and genetic features of a PFIC1 patient definitely diagnosed by ATP8B1 genetic analysis. The patient, a boy aged 14 months, was referred to the hospital with the complaint of jaundiced skin and sclera over 10 months. The patient had been managed in different hospitals, but the therapeutic effects were unsatisfactory due to undetermined etiology. On physical examination, hepatosplenomegaly was discovered in addition to jaundice of the skin and sclera. The liver was palpable 4 cm below the right subcostal margin and 2 cm below the xiphoid while the spleen 2 cm below the left subcostal margin. The liver function test revealed elevated levels of serum total bile acids, bilirubin, and transaminases; however, the γ-glutamyl transferase level was normal. The diagnosis was genetic cholestasis of undetermined origin. At the age of 1 year and 8 months, a Roux-en-Y cholecystocolonic bypass operation was performed, and thereafter the jaundice disappeared. At 5 years and 1 month, via whole genome sequencing analysis and Sanger sequencing confirmation, the boy was found to be a homozygote of mutation c.2081T>A(p.I694N) of ATP8B1 gene, and thus PFIC1 was definitely diagnosed. The boy was followed up until he was 6 years, and jaundice did not recur, but the long-term outcome remains to be observed. PMID:27530795

  17. EARLINET dust observations vs. BSC-DREAM8b modeled profiles: 12-year-long systematic comparison at Potenza, Italy

    NASA Astrophysics Data System (ADS)

    Mona, L.; Papagiannopoulos, N.; Basart, S.; Baldasano, J.; Binietoglou, I.; Cornacchia, C.; Pappalardo, G.

    2014-08-01

    In this paper, we report the first systematic comparison of 12-year modeled dust extinction profiles vs. Raman lidar measurements. We use the BSC-DREAM8b model, one of the most widely used dust regional models in the Mediterranean, and Potenza EARLINET lidar profiles for Saharan dust cases, the largest one-site database of dust extinction profiles. A total of 310 dust cases were compared for the May 2000-July 2012 period. The model reconstructs the measured layers well: profiles are correlated within 5% of significance for 60% of the cases and the dust layer center of mass as measured by lidar and modeled by BSC-DREAM8b differ on average 0.3 ± 1.0 km. Events with a dust optical depth lower than 0.1 account for 70% of uncorrelated profiles. Although there is good agreement in terms of profile shape and the order of magnitude of extinction values, the model overestimates the occurrence of dust layer top above 10 km. Comparison with extinction profiles measured by the Raman lidar shows that BSC-DREAM8b typically underestimates the dust extinction coefficient, in particular below 3 km. Lowest model-observation differences (below 17%) correspond to a lidar ratio at 532 nm and Ångström exponent at 355/532 nm of 60 ± 13 and 0.1 ± 0.6 sr, respectively. These are in agreement with values typically observed and modeled for pure desert dust. However, the highest differences (higher than 85%) are typically related to greater Ångström values (0.5 ± 0.6), denoting smaller particles. All these aspects indicate that the level of agreement decreases with an increase in mixing/modification processes.

  18. Pigs fed camelina meal increase hepatic gene expression of cytochrome 8b1, aldehyde dehydrogenase, and thiosulfate transferase

    PubMed Central

    2014-01-01

    Camelina sativa is an oil seed crop which can be grown on marginal lands. Camelina seed oil is rich in omega-3 fatty acids (>35%) and γ-tocopherol but is also high in erucic acid and glucosinolates. Camelina meal, is the by-product after the oil has been extracted. Camelina meal was fed to 28 d old weaned pigs at 3.7% and 7.4% until age 56 d. The camelina meal supplements in the soy based diets, improved feed efficiency but also significantly increased the liver weights. Gene expression analyses of the livers, using intra-species microarrays, identified increased expression of phase 1 and phase 2 drug metabolism enzymes. The porcine versions of the enzymes were confirmed by real time PCR. Cytochrome 8b1 (CYP8B1), aldehyde dehydrogenase 2 (Aldh2), and thiosulfate transferase (TST) were all significantly stimulated. Collectively, these genes implicate the camelina glucosinolate metabolite, methyl-sulfinyldecyl isothiocyanate, as the main xeniobiotic, causing increased hepatic metabolism and increased liver weight. PMID:24383433

  19. A 727 airplane center duct inlet low speed performance confirmation model test for refanned JT8D engines, phase 2

    NASA Technical Reports Server (NTRS)

    Kaldschmidt, G.; Syltebo, B. E.; Ting, C. T.

    1973-01-01

    The results from testing of a 0.3 scale model center duct inlet (S duct) for the Pratt and Whitney Aircraft JT8D-100 engines are presented. The objective of this test was to demonstrate that the required airflow of the JT8D-100 engine (480 lb/sec as compared to 334 lb/sec for JT8D-15) can be achieved with minimum modifications to the existing 727 airplane structure at acceptable levels of total pressure recovery and distortion. Steady-state pressure recovery, steady-state pressure distortion, and dynamic pressure measurements were taken at the engine face station. Surface static pressure measurements were taken along the duct. Test results indicated that the required airflow was achieved with acceptable pressure recovery (comparable to the current 727-200 S duct). Inlet inflow angle variation within the 727 airplane operating regime (minus 5 to 5 degrees) had no effect on the inlet performance. Pressure distortion at static and forward speed at takeoff airflow conditions are within P and WA limits for the Phase II duct when equipped with vortex generators. Static crosswind operation between 10 knots and 25 knots appears feasible at full takeoff power.

  20. Heterologous expression of ATG8c from soybean confers tolerance to nitrogen deficiency and increases yield in Arabidopsis.

    PubMed

    Xia, Tongmei; Xiao, Dong; Liu, Dong; Chai, Wenting; Gong, Qingqiu; Wang, Ning Ning

    2012-01-01

    Nitrogen is an essential element for plant growth and yield. Improving Nitrogen Use Efficiency (NUE) of crops could potentially reduce the application of chemical fertilizer and alleviate environmental damage. To identify new NUE genes is therefore an important task in molecular breeding. Macroautophagy (autophagy) is an intracellular process in which damaged or obsolete cytoplasmic components are encapsulated in double membraned vesicles termed autophagosomes, then delivered to the vacuole for degradation and nutrient recycling. One of the core components of autophagosome formation, ATG8, has been shown to directly mediate autophagosome expansion, and the transcript of which is highly inducible upon starvation. Therefore, we postulated that certain homologs of Saccharomyces cerevisiae ATG8 (ScATG8) from crop species could have potential for NUE crop breeding. A soybean (Glycine max, cv. Zhonghuang-13) ATG8, GmATG8c, was selected from the 11 family members based on transcript analysis upon nitrogen deprivation. GmATG8c could partially complement the yeast atg8 mutant. Constitutive expression of GmATG8c in soybean callus cells not only enhanced nitrogen starvation tolerance of the cells but accelerated the growth of the calli. Transgenic Arabidopsis over-expressing GmATG8c performed better under extended nitrogen and carbon starvation conditions. Meanwhile, under optimum growth conditions, the transgenic plants grew faster, bolted earlier, produced larger primary and axillary inflorescences, eventually produced more seeds than the wild-type. In average, the yield was improved by 12.9%. We conclude that GmATG8c may serve as an excellent candidate for breeding crops with enhanced NUE and better yield. PMID:22629371

  1. A Validated HPLC Method for Simultaneous Determination of Caffeoyl Phenylethanoid Glucosides and Flavone 8-C-glycosides in Haberlea rhodopensis.

    PubMed

    Zheleva-Dimitrova, Dimitrina; Nedialkov, Paraskev; Giresser, Ulrich

    2016-06-01

    A HPLC-UV method for analysis of the main compounds: caffeoyl phenylethanoid glucosides myconoside (1) and paucifloside (2) and flavone 8-C-glycosides: hispidulin 8-C-β-galactopyranoside (3), hispidulin 8-C-(2"-O-syringoyl-β-glucopyranoside) (4), hispidulin 8-C-(6-O-acetyl-β-glucopyranoside) (5) and hispidulin 8-C-(6-O-acetyl-2"-O-syringoyl--glucopyranoside) (6) in Haberlea rhodopensis leaves was developed and validated. Compound 3 was isolated for the first time from the title species. Ultrasound extraction with 80% methanol at room temperature allowed a good recovery of analytes (from 87.2 % for 1 to 109.8 % for 3) and the precision of the entire procedure was between 1.6% and 6.9%. The subsequent HPLC separation and quantification was achieved using a Hypersil ODS C18 column and UV detection at 280 nm. The mobile phase comprised methanol and 0.1 % o-phosphoric acid, and gradient elution mode was applied. The detection limits ranged from 0.042 μg/mL (6) to 0.18 μg/mL (5). The total amount in leaves of the assayed phenolic compounds was 374.2 mg/g. Myconoside was found to be the dominant compound in H. rhodopensis extract (332.2 ± 0.7 mg/g dw) and reached up to 88.8% of the analyzed mixture in leaves, while the total content of flavone C-glycosides was 17.1 mg/g dw. PMID:27534117

  2. Screening a phage display library for a novel FGF8b-binding peptide with anti-tumor effect on prostate cancer

    SciTech Connect

    Wang, Wenhui; Chen, Xilei; Li, Tao; Li, Yanmei; Wang, Ruixue; He, Dan; Luo, Wu; Li, Xiaokun; Wu, Xiaoping

    2013-05-01

    Fibroblast growth factor 8b (FGF8b) is the major isoform of FGF8 expressed in prostate cancer and it correlates with the stage and grade of the disease. FGF8b has been considered as a potential target for prostate cancer therapy. Here we isolated 12 specific FGF8b-binding phage clones by screening a phage display heptapeptide library with FGF8b. The peptide (HSQAAVP, named as P12) corresponding to one of these clones showed high homology to the immunoglobulin-like (Ig-like) domain II(D2) of high-affinity FGF8b receptor (FGFR3c), contained 3 identical amino acids (AVP) to the authentic FGFR3 D2 sequence aa 163–169 (LLAVPAA) directly participating in ligand binding, carried the same charges as its corresponding motif (aa163–169) in FGFR3c, suggesting that P12 may have a greater potential to interrupt FGF8b binding to its receptors than other identified heptapeptides do. Functional analysis indicated that synthetic P12 peptides mediate significant inhibition of FGF8b-induced cell proliferation, arrest cell cycle at the G0/G1 phase via suppression of Cyclin D1 and PCNA, and blockade of the activations of Erk1/2 and Akt cascades in both prostate cancer cells and vascular endothelial cells. The results demonstrated that the P12 peptide acting as an FGF8b antagonist may have therapeutic potential in prostate cancer. - Highlights: ► A novel FGF8b-binding peptide P12 was isolated from a phage display library. ► The mechanisms for P12 peptide inhibiting cell proliferation were proposed. ► P12 caused cell cycle arrest at G0/G1 phase via suppression of Cyclin D1 and PCNA. ► P12 suppressed FGF8b-induced activations of Akt and MAP kinases. ► P12 acting as an FGF8b antagonist may have therapeutic potential in prostate cancer.

  3. Measurement of the total active 8B solar neutrino flux at the Sudbury Neutrino Observatory with enhanced neutral current sensitivity.

    PubMed

    Ahmed, S N; Anthony, A E; Beier, E W; Bellerive, A; Biller, S D; Boger, J; Boulay, M G; Bowler, M G; Bowles, T J; Brice, S J; Bullard, T V; Chan, Y D; Chen, M; Chen, X; Cleveland, B T; Cox, G A; Dai, X; Dalnoki-Veress, F; Doe, P J; Dosanjh, R S; Doucas, G; Dragowsky, M R; Duba, C A; Duncan, F A; Dunford, M; Dunmore, J A; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Formaggio, J A; Fowler, M M; Frame, K; Fulsom, B G; Gagnon, N; Graham, K; Grant, D R; Hahn, R L; Hall, J C; Hallin, A L; Hallman, E D; Hamer, A S; Handler, W B; Hargrove, C K; Harvey, P J; Hazama, R; Heeger, K M; Heintzelman, W J; Heise, J; Helmer, R L; Hemingway, R J; Hime, A; Howe, M A; Jagam, P; Jelley, N A; Klein, J R; Kos, M S; Krumins, A V; Kutter, T; Kyba, C C M; Labranche, H; Lange, R; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Levine, I; Luoma, S; MacLellan, R; Majerus, S; Mak, H B; Maneira, J; Marino, A D; McCauley, N; McDonald, A B; McGee, S; McGregor, G; Mifflin, C; Miknaitis, K K S; Miller, G G; Moffat, B A; Nally, C W; Nickel, B G; Noble, A J; Norman, E B; Oblath, N S; Okada, C E; Ollerhead, R W; Orrell, J L; Oser, S M; Ouellet, C; Peeters, S J M; Poon, A W P; Robertson, B C; Robertson, R G H; Rollin, E; Rosendahl, S S E; Rusu, V L; Schwendener, M H; Simard, O; Simpson, J J; Sims, C J; Sinclair, D; Skensved, P; Smith, M W E; Starinsky, N; Stokstad, R G; Stonehill, L C; Tafirout, R; Takeuchi, Y; Tesić, G; Thomson, M; Thorman, M; Van Berg, R; Van de Water, R G; Virtue, C J; Wall, B L; Waller, D; Waltham, C E; Tseung, H Wan Chan; Wark, D L; West, N; Wilhelmy, J B; Wilkerson, J F; Wilson, J R; Wouters, J M; Yeh, M; Zuber, K

    2004-05-01

    The Sudbury Neutrino Observatory has precisely determined the total active (nu(x)) 8B solar neutrino flux without assumptions about the energy dependence of the nu(e) survival probability. The measurements were made with dissolved NaCl in heavy water to enhance the sensitivity and signature for neutral-current interactions. The flux is found to be 5.21 +/- 0.27(stat)+/-0.38(syst) x 10(6) cm(-2) s(-1), in agreement with previous measurements and standard solar models. A global analysis of these and other solar and reactor neutrino results yields Deltam(2)=7.1(+1.2)(-0.6) x 10(-5) eV(2) and theta=32.5(+2.4)(-2.3) degrees. Maximal mixing is rejected at the equivalent of 5.4 standard deviations.

  4. Parametric Study of a YAV-8B Harrier in Ground Effect using Time-Dependent Navier-Stokes Computations

    NASA Technical Reports Server (NTRS)

    Pandya, Shishir; Chaderjian, Neal; Ahmad, Jasim; Kwak, Dochan (Technical Monitor)

    2002-01-01

    A process is described which enables the generation of 35 time-dependent viscous solutions for a YAV-8B Harrier in ground effect in one week. Overset grids are used to model the complex geometry of the Harrier aircraft and the interaction of its jets with the ground plane and low-speed ambient flow. The time required to complete this parametric study is drastically reduced through the use of process automation, modern computational platforms, and parallel computing. Moreover, a dual-time-stepping algorithm is described which improves solution robustness. Unsteady flow visualization and a frequency domain analysis are also used to identify and correlated key flow structures with the time variation of lift.

  5. Moving-base simulation evaluation of thrust margins for vertical landing for the NASA YAV-8B Harrier aircraft

    NASA Technical Reports Server (NTRS)

    Franklin, James A.; Stortz, Michael W.

    1993-01-01

    A simulation experiment was conducted on Ames Research Center's Vertical Motion Simulator to evaluate the thrust margin for vertical landing required for the YAV-8B Harrier. Two different levels of ground effect were employed, representing the aircraft with or without lift improvement devices installed. In addition, two different inlet temperature profiles were included to cover a wide range of hot gas ingestion. For each ground effect and hot gas ingestion variant, vertical landings were performed at successively heavier weights, with the pilot assessing the acceptability of the operation in each case. Results are presented as a function of hover weight ratio and a metric of the mean ground effect and ingestion that reflect the increase in thrust margin required to provide acceptable control of sink rate during the descent to touchdown with increasing suck down and hot gas ingestion.

  6. Critical assessment of the claim of a significant difference between the results of measurements of the Coulomb dissociation of {sup 8}B and the {sup 7}Be(p,{gamma}){sup 8}B direct capture reaction

    SciTech Connect

    Gai, Moshe

    2006-08-15

    The Coulomb dissociation (CD) of {sup 8}B has emerged as a landmark testing ground of the very method of CD for measuring the cross section of the low-energy {sup 7}Be(p,{gamma}){sup 8}B direct capture (DC) reaction. Recent claims of evidence of slope difference between CD and DC results are critically examined. We include all relevant RIKEN2 data and all previously published DC data, and we examine the extracted so-called average scale-independent slope (b). The parametrization used by the Seattle group to extract the so-called b-slope parameter is also examined at energies above 300 keV. Considering the physical slope (S{sup '}=dS/dE) above 300 keV, we observe a (1.7{sigma}) agreement between slopes (S{sup '}) measured in CD and DC above 300 keV. The claim that S{sub 17}(0) values extracted from CD data are inconsistent and lower than DC results arises from a neglect of substantial systematic uncertainty of low-energy CD data. A consideration of the published CD S{sub 17}(0) results yields very consistent S{sub 17}(0) values that agree with most recent DC measurements. The recent correction of the b-slope parameter suggested by Esbensen, Bertsch, and Snover (EBS) was applied to the wrong b slope calculated using part of the RIKEN2 data. When the correct slope of the RIKEN2 data is used, the EBS correction in fact leads to a substantial disagreement between the slopes of the RIKEN2 data and DC data. In spite of an agreement between CD and DC data neither allow for extracting the slope above 300 keV with high accuracy. Uncertainty of the slope (S{sup '}) leads to an additional uncertainty of the extrapolated S{sub 17}(0). The slope of the astrophysical cross-section factor S{sub 17} must be measured with high precision to enable extraction of the d/s ratio and a high-precision extrapolation of S{sub 17}(0)

  7. Effect of ceramic coating of JT8D combustor liner on maximum liner temperatures and other combustor performance parameters

    NASA Technical Reports Server (NTRS)

    Butze, H. F.; Liebert, C. H.

    1976-01-01

    The effect of ceramic coating of a JT8D combustor liner was investigated at simulated cruise and takeoff conditions with two fuels of widely different aromatic contents. Substantial decreases in maximum liner temperatures and flame radiation values were obtained with the ceramic-coated liner. Small reductions in exhaust gas smoke concentrations were observed with the ceramic-coated liner. Other performance parameters such as combustion efficiency and emissions of unburned hydrocarbons, CO, and NOx were not affected significantly. No deterioration of the ceramic coating was observed after about 6 hours of cyclic operation including several startups and shutdowns.

  8. Phase 2 program on ground test of refanned JT8D turbofan engines and nacelles for the 727 airplane. Volume 3: Ground tests

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The NASA Refan Program included full-scale performance and noise ground tests of both a current production (JT8D-15) and a refanned (JT8D-115) engine. A description of the two ground tests including detailed propulsion, noise, and structural test results is presented. The primary objectives of the total test program were comparison of JT8D-15 and JT8D-115 overall propulsion system performance and noise characteristics and determination of incremental component noise levels. Other objectives of the test program included: (1) determination of acoustic treatment effectiveness; (2) measurement of internal sound pressure levels; (3) measurement of inlet and exhaust hardware performance; (4) determination of center-engine surge margin; and (5) evaluation of certain structural characteristics associated with the 727 refan center-engine inlet duct and JT8D refan engine exhaust system. The JT8D-15 and -115 tests were conducted during September 1974 and January to March 1975, respectively. Analyses of the test data indicated that the JT8D-115, as compared to the JT8D-15, demonstrates a 12.5 percent to 13.2 percent reduction in static specific fuel consumption, and a reduction of 6 to 7 PNdB in a weighted average value of static tone corrected perceived noise level. Separated into noise components, a significant reduction was shown for the inlet fan, aft fan, exhaust duct flow, turbine, and jet noises. However, core noise was increased. Photographs of test stands and test equipment are shown.

  9. A sub-micrometer resolution hard X-ray microprobe system of BL8C at Pohang Light Source.

    PubMed

    Sung, Nark Eon; Lee, Ik Jae; Lee, Kug Seong; Jeong, Seong Hun; Kang, Seen Woong; Shin, Yong Bi

    2015-09-01

    A microprobe system has been installed on the nanoprobe/XAFS beamline (BL8C) at PLS-II, South Korea. Owing to the reproducible switch of the gap of the in-vacuum undulator (IVU), the intense and brilliant hard X-ray beam of an IVU can be used in X-ray fluorescence (XRF) and X-ray absorption fine-structure (XAFS) experiments. For high-spatial-resolution microprobe experiments a Kirkpatrick-Baez mirror system has been used to focus the millimeter-sized X-ray beam to a micrometer-sized beam. The performance of this system was examined by a combination of micro-XRF imaging and micro-XAFS of a beetle wing. These results indicate that the microprobe system of the BL8C can be used to obtain the distributions of trace elements and chemical and structural information of complex materials.

  10. Design and development experience with a digital fly-by-wire control system in an F-8C airplane

    NASA Technical Reports Server (NTRS)

    Deets, D. A.

    1976-01-01

    To assess the feasibility of a digital fly by wire system, the mechanical flight control system of an F-8C airplane was replaced with a digital primary system and an analog backup system. The Apollo computer was used as the heart of the primary system. This paper discusses the experience gained during the design and development of the system and relates it to active control systems that are anticipated for future civil transport applications.

  11. Design and development experience with a digital fly-by-wire control system in an F-8C airplane

    NASA Technical Reports Server (NTRS)

    Deets, D. A.

    1975-01-01

    To assess the feasibility of a digital fly-by-wire system, the mechanical flight control system of an F-8C airplane was replaced with a digital system and an analog backup system. The Apollo computer was used as the heart of the primary system. This paper discusses the experience gained during the design and development of the system and relates it to active control systems that are anticipated for future civil transport applications.

  12. The stochastic control of the F-8C aircraft using the Multiple Model Adaptive Control (MMAC) method

    NASA Technical Reports Server (NTRS)

    Athans, M.; Dunn, K. P.; Greene, E. S.; Lee, W. H.; Sandel, N. R., Jr.

    1975-01-01

    The purpose of this paper is to summarize results obtained for the adaptive control of the F-8C aircraft using the so-called Multiple Model Adaptive Control method. The discussion includes the selection of the performance criteria for both the lateral and the longitudinal dynamics, the design of the Kalman filters for different flight conditions, the 'identification' aspects of the design using hypothesis testing ideas, and the performance of the closed loop adaptive system.

  13. The 727 airplane side inlet low-speed performance confirmation model test for refanned JT8D engines

    NASA Technical Reports Server (NTRS)

    Schuehle, A. L.

    1974-01-01

    The results of a low-speed wind tunnel test of a 0.3 scale model 727 airplane side inlet for JT8D-100 engines are presented. The objectives of the test were to develop lines for a full-scale flightworthy inlet, to evaluate inlet total pressure recovery and steady-state total pressure distortion, and to obtain model-scale distortion data which can be used in the assessment of the compatibility of the inlet with the JT8D-100 series engines. A secondary objective was to obtain internal/external cowl static pressures for the determination of nacelle loads. Two basic inlet models were tested at static, forward speed, angle-of-attack (inflow angle), and cross-wind conditions. One model was with and one without an acoustic ring. Two modifications to the models were also tested, one with the ring closer to the inlet throat and one with a larger lip. Test measurements consisted of inlet surface static pressure, engine face total pressure, inlet airflow, tunnel total pressure, tunnel total temperature and tunnel velocity. Total pressure traverses were taken directly behind the ring and strut. No dynamic measurements were taken.

  14. {sup 7}Be(p,{gamma}){sup 8}B S factor from ab initio no-core shell model wave functions

    SciTech Connect

    Navratil, P.; Bertulani, C.A.; Caurier, E.

    2006-06-15

    Nuclear structure of {sup 7}Be, {sup 8}B, and {sup 7,8}Li is studied within the ab initio no-core shell model (NCSM). Starting from high-precision nucleon-nucleon (NN) interactions, wave functions of {sup 7}Be and {sup 8}B bound states are obtained in basis spaces up to 10({Dirac_h}/2{pi}){omega} and used to calculate channel cluster form factors (overlap integrals) of the {sup 8}B ground state with {sup 7}Be+p. Due to the use of the harmonic oscillator (HO) basis, the overlap integrals have incorrect asymptotic properties. We fix this problem in two alternative ways. First, by a Woods-Saxon potential solution fit to the interior of the NCSM overlap integrals. Second, by a direct matching with the Whittaker function. The corrected overlap integrals are then used for the {sup 7}Be(p,{gamma}){sup 8}B S-factor calculation. We study the convergence of the S factor with respect to the NCSM HO frequency and the model space size. Our S factor results agree with recent direct measurement data. We also test the spectroscopic factors and the corrected overlap integrals from the NCSM in describing the momentum distributions in knockout reactions with {sup 8}B projectiles. A good agreement with the available experimental data is also found, attesting to the overall consistency of the calculations.

  15. Volumetric Properties of the Mixture Toluene C7H8 + C14H30 Tetradecane (VMSD1212, LB3260_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume A 'Binary Liquid Systems of Nonelectrolytes I' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Toluene C7H8 + C14H30 Tetradecane (VMSD1212, LB3260_V)' providing data by calculation of molar excess volume from low-pressure density measurements at variable mole fraction and constant temperature.

  16. Volumetric Properties of the Mixture Toluene C7H8 + C14H30 Tetradecane (VMSD1111, LB3270_V)

    NASA Astrophysics Data System (ADS)

    Cibulka, I.; Fontaine, J.-C.; Sosnkowska-Kehiaian, K.; Kehiaian, H. V.

    This document is part of Subvolume A 'Binary Liquid Systems of Nonelectrolytes I' of Volume 26 'Heats of Mixing, Vapor-Liquid Equilibrium, and Volumetric Properties of Mixtures and Solutions' of Landolt-Börnstein Group IV 'Physical Chemistry'. It contains the Chapter 'Volumetric Properties of the Mixture Toluene C7H8 + C14H30 Tetradecane (VMSD1111, LB3270_V)' providing data from direct low-pressure measurement of mass density at variable mole fraction and constant temperature, in the single-phase region(s).

  17. Parametric Time-Dependent Navier-Stokes Computations for a YAV-8B Harrier in Ground Effect

    NASA Technical Reports Server (NTRS)

    Chaderjian, Neal M.; Pandya, Shishir; Ahmad, Jasim; Murman, Scott; Kwak, Dochan (Technical Monitor)

    2002-01-01

    The Harrier Jump Jet has the distinction of being the only powered-lift aircraft in the free world to achieve operational status and to have flown in combat. This V/STOL aircraft can take-off and land vertically or utilize very short runways by directing its four exhaust nozzles towards the ground. Transition to forward flight is achieved by rotating these nozzles into a horizontal position. Powered-lift vehicles have certain advantages over conventional strike fighters. Their V/STOL capabilities allow for safer carrier operations, smaller carrier size, and quick reaction time for troop support. Moreover, they are not dependent on vulnerable land-based runways. The AV-8A Harrier first entered service in the British Royal Air Force (RAF) during 1969, and the U.S. Marine Corps (USMC) in 1971. The AV-8B was a redesign to achieve improved payload capacity, range, and accuracy. This modified design first entered service with the USMC and RAF in 1985. The success and unique capabilities of the Harrier has prompted the design of a powered-lift version of the Joint Strike Fighter (JSF). The flowfield for the Harrier near the ground during low-speed or hover flight operations is very complex and time-dependent. A sketch of this flowfield is shown. Warm air from the fan is exhausted from the front nozzles, while a hot air/fuel mixture from the engine is exhausted from the rear nozzles. These jets strike the ground and move out radially forming a ground jet-flow. The ambient freestream, due to low-speed forward flight or - headwind during hover, opposes the jet-flow. This interaction causes the flow to separate and form a ground vortex. The multiple jets also interact with each other near the ground and form an upwash or jet fountain, which strikes the underside of the fuselage. If the aircraft is sufficiently close to the ground, the inlet can ingest ground debris and hot gases from the fountain and ground vortex. This Hot Gas Ingestion (HGI) can cause a sudden loss of

  18. The effects of ordered carbon vacancies on stability and thermo-mechanical properties of V8C7 compared with VC

    PubMed Central

    Chong, XiaoYu; Jiang, YeHua; Zhou, Rong; Feng, Jing

    2016-01-01

    The ordered non-stoichiometric V8C7 can form in the VCy carbides by the disorder–order phase transformation. The intrusion of ordered carbon vacancies can affect their stability, mechanical, thermal and electronic properties. The relatively thermodynamic stability and mechanical properties at high temperature for the ordered stoichiometric VC and non-stoichiometric V8C7 are investigated in this paper by first-principle calculations combined with the quasi-harmonic approximation. The difference between the properties of VC and V8C7 can be obtained. We find that the V8C7 is thermodynamic more stable than VC, but has weaker elastic heat resistance than VC. Moreover, the minimum thermal conductivity of VC is a little larger than V8C7 and a simple way is proposed to characterize the anisotropy of lattice thermal conductivity based on the Cahill’s model. PMID:27659072

  19. 26 CFR 31.3401(a)(8)(B)-1 - Remuneration for services performed in possession of the United States (other than Puerto Rico...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 15 2010-04-01 2010-04-01 false Remuneration for services performed in... TAX AT SOURCE Collection of Income Tax at Source § 31.3401(a)(8)(B)-1 Remuneration for services...) Remuneration paid for services for an employer (other than the United States or any agency thereof)...

  20. Association between chromosomal aberration of COX8C and tethered spinal cord syndrome: array-based comparative genomic hybridization analysis

    PubMed Central

    Zhao, Qiu-jiong; Bai, Shao-cong; Cheng, Cheng; Tao, Ben-zhang; Wang, Le-kai; Liang, Shuang; Yin, Ling; Hang, Xing-yi; Shang, Ai-jia

    2016-01-01

    Copy number variations have been found in patients with neural tube abnormalities. In this study, we performed genome-wide screening using high-resolution array-based comparative genomic hybridization in three children with tethered spinal cord syndrome and two healthy parents. Of eight copy number variations, four were non-polymorphic. These non-polymorphic copy number variations were associated with Angelman and Prader-Willi syndromes, and microcephaly. Gene function enrichment analysis revealed that COX8C, a gene associated with metabolic disorders of the nervous system, was located in the copy number variation region of Patient 1. Our results indicate that array-based comparative genomic hybridization can be used to diagnose tethered spinal cord syndrome. Our results may help determine the pathogenesis of tethered spinal cord syndrome and prevent occurrence of this disease.

  1. Association between chromosomal aberration of COX8C and tethered spinal cord syndrome: array-based comparative genomic hybridization analysis

    PubMed Central

    Zhao, Qiu-jiong; Bai, Shao-cong; Cheng, Cheng; Tao, Ben-zhang; Wang, Le-kai; Liang, Shuang; Yin, Ling; Hang, Xing-yi; Shang, Ai-jia

    2016-01-01

    Copy number variations have been found in patients with neural tube abnormalities. In this study, we performed genome-wide screening using high-resolution array-based comparative genomic hybridization in three children with tethered spinal cord syndrome and two healthy parents. Of eight copy number variations, four were non-polymorphic. These non-polymorphic copy number variations were associated with Angelman and Prader-Willi syndromes, and microcephaly. Gene function enrichment analysis revealed that COX8C, a gene associated with metabolic disorders of the nervous system, was located in the copy number variation region of Patient 1. Our results indicate that array-based comparative genomic hybridization can be used to diagnose tethered spinal cord syndrome. Our results may help determine the pathogenesis of tethered spinal cord syndrome and prevent occurrence of this disease. PMID:27651783

  2. Performance of Diffusion Aluminide Coatings Applied on Alloy CF8C-Plus at 800oC

    SciTech Connect

    Kumar, Deepak; Dryepondt, Sebastien N; Zhang, Ying; Haynes, James A; Pint, Bruce A; Armstrong, Beth L; Shyam, Amit; Lara-Curzio, Edgar

    2012-01-01

    High performance cast stainless steel, CF8C-Plus, is a low cost alloy with prospective applications ranging from covers and casings of small and medium size gas turbines to turbocharger housing and manifolds in internal combustion engines. Diffusion aluminide coatings were applied on this alloy as a potential strategy for improved oxidation resistance, particularly in wet air and steam. In this paper the performance of the aluminide coatings evaluated by cyclic oxidation experiments in air containing 10 vol.% H2O at 800 C and conventional tension-compression low-cycle-fatigue tests in air at 800 C with a strain range of 0.5% is presented. The results show that specimens coated by a chemical vapor deposition process provide better oxidation resistance than those coated by an Al-slurry coating process. The application of a coating by pack cementation reduced the fatigue life by 15%.

  3. A reliable dual-redundant sensor FDI system for the NASA F8C-DFBW aircraft

    NASA Technical Reports Server (NTRS)

    Deckert, J. C.; Desai, M. N.; Deyst, J. J., Jr.; Willsky, A. S.

    1976-01-01

    In this paper we outline the structure of a sensor failure detection and identification (FDI) system designed for the NASA F8C DFBW aircraft. The system is for use in a dual-redundant environment, and it takes maximal advantage of all functional and kinematic relationships among the sensed variables. The technique is reliable in that we have designed the system to account for a variety of unmodeled effects and have introduced a new concept - the quality sequential probability ratio (QSPR) - that provides a useful measure of our confidence in each test. The methodology used for determining detector parameters and the 'outer loop logic' for combining the various sources of information are described.

  4. Dual redundant sensor FDI techniques applied to the NASA F8C DFBW aircraft. [Failure Detection and Identification

    NASA Technical Reports Server (NTRS)

    Desai, M. N.; Deckert, J. C.; Deyst, J. J.; Willsky, A. S.; Chow, E. Y.

    1976-01-01

    An onboard failure detection and identification (FDI) technique for dual redundant sensors on the NASA F8C digital fly-by-wire (DFBW) aircraft is presented. The failure of one of a pair of sensors of the same type is detected by a direct redundancy trigger which observes the difference between the outputs of these two sensors. Identification of the failed sensor is accomplished utilizing the analytic redundancy that exists as kinematic and functional relationships among the variables being measured by dissimilar instruments. In addition, identification of generic failures, common to both instruments of a given type, is accomplished by using a time trigger to periodically initiate analytic redundancy failure identification tests for individual sensors. The basic form of these tests is the comparison of the measurement of a variable using the suspect instrument with another measurement of the same variable obtained using other instrument types.

  5. Preliminary system design study for a digital fly-by-wire flight control system for an F-8C aircraft

    NASA Technical Reports Server (NTRS)

    Seacord, C. L.; Vaughn, D. K.

    1976-01-01

    The design of a fly-by-wire control system having a mission failure probability of less than one millionth failures per flight hour is examined. Emphasis was placed on developing actuator configurations that would improve the system performance, and consideration of the practical aspects of sensor/computer and computer/actuator interface implementation. Five basic configurations were defined as appropriate candidates for the F-8C research aircraft. Options on the basic configurations were included to cover variations in flight sensors, redundancy levels, data transmission techniques, processor input/output methods, and servo actuator arrangements. The study results can be applied to fly by wire systems for transport aircraft in general and the space shuttle.

  6. NASA/RAE collaboration on nonlinear control using the F-8C digital fly-by-wire aircraft

    NASA Technical Reports Server (NTRS)

    Butler, G. F.; Corbin, M. J.; Mepham, S.; Stewart, J. F.; Larson, R. R.

    1983-01-01

    Design procedures are reviewed for variable integral control to optimize response (VICTOR) algorithms and results of preliminary flight tests are presented. The F-8C aircraft is operated in the remotely augmented vehicle (RAV) mode, with the control laws implemented as FORTRAN programs on a ground-based computer. Pilot commands and sensor information are telemetered to the ground, where the data are processed to form surface commands which are then telemetered back to the aircraft. The RAV mode represents a singlestring (simplex) system and is therefore vulnerable to a hardover since comparison monitoring is not possible. Hence, extensive error checking is conducted on both the ground and airborne computers to prevent the development of potentially hazardous situations. Experience with the RAV monitoring and validation procedures is described.

  7. Association between chromosomal aberration of COX8C and tethered spinal cord syndrome: array-based comparative genomic hybridization analysis.

    PubMed

    Zhao, Qiu-Jiong; Bai, Shao-Cong; Cheng, Cheng; Tao, Ben-Zhang; Wang, Le-Kai; Liang, Shuang; Yin, Ling; Hang, Xing-Yi; Shang, Ai-Jia

    2016-08-01

    Copy number variations have been found in patients with neural tube abnormalities. In this study, we performed genome-wide screening using high-resolution array-based comparative genomic hybridization in three children with tethered spinal cord syndrome and two healthy parents. Of eight copy number variations, four were non-polymorphic. These non-polymorphic copy number variations were associated with Angelman and Prader-Willi syndromes, and microcephaly. Gene function enrichment analysis revealed that COX8C, a gene associated with metabolic disorders of the nervous system, was located in the copy number variation region of Patient 1. Our results indicate that array-based comparative genomic hybridization can be used to diagnose tethered spinal cord syndrome. Our results may help determine the pathogenesis of tethered spinal cord syndrome and prevent occurrence of this disease. PMID:27651783

  8. Aerodynamic performance of 0.4066-scale model of JT8D refan stage with S-duct inlet

    NASA Technical Reports Server (NTRS)

    Moore, R. D.; Kovich, G.; Lewis, G. W., Jr.

    1977-01-01

    A scale model of the JT8D refan stage was tested with a scale model of the S-duct inlet design for the refanned Boeing 727 center engine. Detailed survey data of pressures, temperatures, and flow angles were obtained over a range of flows at speeds from 70 to 97 percent of design speed. Two S-duct configurations were tested; one with a bellmouth inlet and the other with a flight lip inlet. The results indicated that the overall performance was essentially unaffected by the distortion generated by the S-duct inlet. The stall weight flow increased by less than 0.5 kg/sec (approximately 1.5% of design flow) with the S-duct inlet compared with that obtained with uniform flow. The detailed measurements indicated that the inlet guide vane (IGV) significantly reduced circumferential variations. For example, the flow angles ahead of the IGV were positive in the right half of the inlet and negative in the left half. Behind the IGV, the flow angles tended to be more uniform circumferentially.

  9. Titanium honeycomb acoustic lining structural and thermal test report. [for acoustic tailpipe for JT8D engine

    NASA Technical Reports Server (NTRS)

    Joynes, D.; Balut, J. P.

    1974-01-01

    The results are presented of static, fatigue and thermal testing of titanium honeycomb acoustic panels representing the acoustic tailpipe for the Pratt and Whitney Aircraft JT8D Refan engine which is being studied for use on the Boeing 727-200 airplane. Test specimens represented the engine and tailpipe flange joints, the rail to which the thrust reverser is attached and shear specimens of the tailpipe honeycomb. Specimens were made in four different batches with variations in configuration, materials and processes in each. Static strength of all test specimens exceeded the design ultimate load requirements. Fatigue test results confirmed that aluminum brazed titanium, as used in the Refan tailpipe design, meets the fatigue durability objectives. Quality of welding was found to be critical to life, with substandard welding failing prematurely, whereas welding within the process specification exceeded the panel skin life. Initial fatigue testing used short grip length bolts which failed prematurely. These were replaced with longer bolts and subsequent testing demonstrated the required life. Thermal tests indicate that perforated skin acoustic honeycomb has approximately twice the heat transfer of solid skin honeycomb.

  10. Identification of fibroblast growth factor-8b target genes associated with early and late cell cycle events in breast cancer cells.

    PubMed

    Nilsson, E M; Brokken, L J S; Narvi, E; Kallio, M J; Härkönen, P L

    2012-07-01

    Fibroblast growth factor-8 (FGF-8) is implicated in the development and progression of breast cancer and its levels are frequently elevated in breast tumors. The mechanisms driving FGF-8-mediated tumorigenesis are not well understood. Herein we aimed to identify target genes associated with FGF-8b-mediated breast cancer cell proliferation by carrying out a cDNA microarray analysis of genes expressed in estrogen receptor negative S115 breast cancer cells treated with FGF-8b for various time periods in comparison with those expressed in non-treated cells. Gene and protein expression was validated for selected genes by qPCR and western blotting respectively. Furthermore, using TRANSBIG data, the expression of human orthologs of FGF-8-regulated genes was correlated to the Nottingham prognostic index and estrogen receptor status. The analysis revealed a number of significantly up- and down-regulated genes in response to FGF-8b at all treatment times. The most differentially expressed genes were genes related to cell cycle regulation, mitosis, cancer, and cell death. Several key regulators of early cell cycle progression such as Btg2 and cyclin D1, as well as regulators of mitosis, including cyclin B, Plk1, survivin, and aurora kinase A, were identified as novel targets for FGF-8b, some of which were additionally shown to correlate with prognosis and ER status in human breast cancer. The results suggest that in stimulation of proliferation FGF-8b not only promotes cell cycle progression through the G1 restriction point but also regulates key proteins involved in chromosomal segregation during mitosis and cytokinesis of breast cancer cells.

  11. Oxidation of Slurry Aluminide Coatings on Cast Stainless Steel Alloy CF8C-Plus at 800oC in Water Vapor

    SciTech Connect

    Haynes, James A; Armstrong, Beth L; Dryepondt, Sebastien N; Kumar, Deepak; Zhang, Ying

    2013-01-01

    A new, cast austenitic stainless steel, CF8C-Plus, has been developed for a wide range of high temperature applications, including diesel exhaust components, turbine casings and turbocharger housings. CF8C-Plus offers significant improvements in creep rupture life and creep rupture strength over standard CF8C steel. However, at higher temperatures and in more aggressive environments, such as those containing significant water vapor, an oxidation-resistant protective coating will be necessary. The oxidation behavior of alloys CF8C and CF8C-Plus with various aluminide coatings were compared at 800oC in air plus 10 vol% water vapor. Due to their affordability, slurry aluminides were the primary coating system of interest, although chemical vapor deposition (CVD) and pack cementation coatings were also compared. Additionally, a preliminary study of the low cycle fatigue behavior of aluminized CF8C-Plus was conducted at 800oC. Each type of coating provided substantial improvements in oxidation behavior, with simple slurry aluminides showing very good oxidation resistance after 4,000 h testing in water vapor. Preliminary low cycle fatigue results indicated that thicker aluminide coatings degraded high temperature fatigue properties of CF8C-Plus, whereas thinner coatings did not. Results suggest that appropriately designed slurry aluminide coatings are a viable option for economical, long-term oxidation protection of austenitic stainless steels in water vapor.

  12. Expression of the Multimeric and Highly Immunogenic Brucella spp. Lumazine Synthase Fused to Bovine Rotavirus VP8d as a Scaffold for Antigen Production in Tobacco Chloroplasts

    PubMed Central

    Alfano, E. Federico; Lentz, Ezequiel M.; Bellido, Demian; Dus Santos, María J.; Goldbaum, Fernando A.; Wigdorovitz, Andrés; Bravo-Almonacid, Fernando F.

    2015-01-01

    Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein which can accommodate foreign polypeptides or protein domains fused to its N-termini, markedly increasing their immunogenicity. The inner core domain (VP8d) of VP8 spike protein from bovine rotavirus is responsible for viral adhesion to sialic acid residues and infection. It also displays neutralizing epitopes, making it a good candidate for vaccination. In this work, the BLS scaffold was assessed for the first time in plants for recombinant vaccine development by N-terminally fusing BLS to VP8d and expressing the resulting fusion (BLSVP8d) in tobacco chloroplasts. Transplastomic plants were obtained and characterized by Southern, northern and western blot. BLSVP8d was highly expressed, representing 40% of total soluble protein (4.85 mg/g fresh tissue). BLSVP8d remained soluble and stable during all stages of plant development and even in lyophilized leaves stored at room temperature. Soluble protein extracts from fresh and lyophilized leaves were able to induce specific neutralizing IgY antibodies in a laying hen model. This work presents BLS as an interesting platform for highly immunogenic injectable, or even oral, subunit vaccines. Lyophilization of transplastomic leaves expressing stable antigenic fusions to BLS would further reduce costs and simplify downstream processing, purification and storage, allowing for more practical vaccines. PMID:26779198

  13. 17beta-estradiol attenuates hyperoxia-induced apoptosis in mouse C8-D1A cell line.

    PubMed

    Huppmann, Stephanie; Römer, Susanne; Altmann, Rodica; Obladen, Michael; Berns, Monika

    2008-11-15

    In premature infants, oxygen free radicals generated following neonatal resuscitation are associated with subsequent diseases such as retinopathy of prematurity and bronchopulmonary dysplasia. Recent studies in brain tissue samples have shown that nonphysiologic oxygen levels play a key role in induction of apoptosis in the developing brain. Estrogen is a well-established agent in neuroprotection and, therefore, is thought to be neuroprotective even in the premature brain. Astrocytes appear to have a critical role in protection and survival of neurons in the brain. As one of the glial cell types, they have a great potential for possible involvement in the mediation of estrogen neuroprotective effects. The aim of our study was to analyze whether astrocytes in cell cultures are damaged by hyperoxia and whether 17beta-estradiol (E2) can protect them against apoptosis. Additionally, we investigated the mechanism of the protection by E2, hypothesizing that it is mediated through extracellular signal-regulated kinase (ERK1/2). Cells underwent eightfold more apoptosis when cultivated in hyperoxia compared with normoxia. Addition of E2 reduced apoptosis in hyperoxia by more than 50%. Levels of ERK1/2 and phosphorylated ERK1/2 were increased after hyperoxia compared with normoxia. Preincubation with E2 prior to exposure to hyperoxia resulted in decreased levels of ERK1/2 and pERK1/2. Hyperoxia induces apoptosis in C8-D1A cells, and E2 seems to be a protecting factor for astrocytes in hyperoxia. This effect is not mediated through up-regulation of pERK1/2.

  14. Heat and corrosion resistant cast CF8C stainless steel with improved high temperature strength and ductility

    DOEpatents

    Maziasz, Philip J.; McGreevy, Tim; Pollard, Michael James; Siebenaler, Chad W.; Swindeman, Robert W.

    2010-08-17

    A CF8C type stainless steel alloy and articles formed therefrom containing about 18.0 weight percent to about 22.0 weight percent chromium and 11.0 weight percent to about 14.0 weight percent nickel; from about 0.05 weight percent to about 0.15 weight percent carbon; from about 2.0 weight percent to about 10.0 weight percent manganese; and from about 0.3 weight percent to about 1.5 weight percent niobium. The present alloys further include less than 0.15 weight percent sulfur which provides high temperature strength both in the matrix and at the grain boundaries without reducing ductility due to cracking along boundaries with continuous or nearly-continuous carbides. The disclosed alloys also have increased nitrogen solubility thereby enhancing strength at all temperatures because nitride precipitates or nitrogen porosity during casting are not observed. The solubility of nitrogen is dramatically enhanced by the presence of manganese, which also retains or improves the solubility of carbon thereby providing additional solid solution strengthening due to the presence of manganese and nitrogen, and combined carbon.

  15. Heat and corrosion resistant cast CF8C stainless steel with improved high temperature strength and ductility

    DOEpatents

    Maziasz, Philip J.; McGreevy, Tim; Pollard, Michael James; Siebenaler, Chad W.; Swindeman, Robert W.

    2006-12-26

    A CF8C type stainless steel alloy and articles formed therefrom containing about 18.0 weight percent to about 22.0 weight percent chromium and 11.0 weight percent to about 14.0 weight percent nickel; from about 0.05 weight percent to about 0.15 weight percent carbon; from about 2.0 weight percent to about 10.0 weight percent manganese; and from about 0.3 weight percent to about 1.5 weight percent niobium. The present alloys further include less than 0.15 weight percent sulfur which provides high temperature strength both in the matrix and at the grain boundaries without reducing ductility due to cracking along boundaries with continuous or nearly-continuous carbides. The disclosed alloys also have increased nitrogen solubility thereby enhancing strength at all temperatures because nitride precipitates or nitrogen porosity during casting are not observed. The solubility of nitrogen is dramatically enhanced by the presence of manganese, which also retains or improves the solubility of carbon thereby providing additional solid solution strengthening due to the presence of manganese and nitrogen, and combined carbon.

  16. Measurement of the rate of nu(e) + d --> p + p + e(-) interactions produced by (8)B solar neutrinos at the Sudbury Neutrino Observatory.

    PubMed

    Ahmad, Q R; Allen, R C; Andersen, T C; Anglin, J D; Bühler, G; Barton, J C; Beier, E W; Bercovitch, M; Bigu, J; Biller, S; Black, R A; Blevis, I; Boardman, R J; Boger, J; Bonvin, E; Boulay, M G; Bowler, M G; Bowles, T J; Brice, S J; Browne, M C; Bullard, T V; Burritt, T H; Cameron, K; Cameron, J; Chan, Y D; Chen, M; Chen, H H; Chen, X; Chon, M C; Cleveland, B T; Clifford, E T; Cowan, J H; Cowen, D F; Cox, G A; Dai, Y; Dai, X; Dalnoki-Veress, F; Davidson, W F; Doe, P J; Doucas, G; Dragowsky, M R; Duba, C A; Duncan, F A; Dunmore, J; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Ferraris, A P; Ford, R J; Fowler, M M; Frame, K; Frank, E D; Frati, W; Germani, J V; Gil, S; Goldschmidt, A; Grant, D R; Hahn, R L; Hallin, A L; Hallman, E D; Hamer, A; Hamian, A A; Haq, R U; Hargrove, C K; Harvey, P J; Hazama, R; Heaton, R; Heeger, K M; Heintzelman, W J; Heise, J; Helmer, R L; Hepburn, J D; Heron, H; Hewett, J; Hime, A; Howe, M; Hykawy, J G; Isaac, M C; Jagam, P; Jelley, N A; Jillings, C; Jonkmans, G; Karn, J; Keener, P T; Kirch, K; Klein, J R; Knox, A B; Komar, R J; Kouzes, R; Kutter, T; Kyba, C C; Law, J; Lawson, I T; Lay, M; Lee, H W; Lesko, K T; Leslie, J R; Levine, I; Locke, W; Lowry, M M; Luoma, S; Lyon, J; Majerus, S; Mak, H B; Marino, A D; McCauley, N; McDonald, A B; McDonald, D S; McFarlane, K; McGregor, G; McLatchie, W; Meijer Drees, R; Mes, H; Mifflin, C; Miller, G G; Milton, G; Moffat, B A; Moorhead, M; Nally, C W; Neubauer, M S; Newcomer, F M; Ng, H S; Noble, A J; Norman, E B; Novikov, V M; O'Neill, M; Okada, C E; Ollerhead, R W; Omori, M; Orrell, J L; Oser, S M; Poon, A W; Radcliffe, T J; Roberge, A; Robertson, B C; Robertson, R G; Rowley, J K; Rusu, V L; Saettler, E; Schaffer, K K; Schuelke, A; Schwendener, M H; Seifert, H; Shatkay, M; Simpson, J J; Sinclair, D; Skensved, P; Smith, A R; Smith, M W; Starinsky, N; Steiger, T D; Stokstad, R G; Storey, R S; Sur, B; Tafirout, R; Tagg, N; Tanner, N W; Taplin, R K; Thorman, M; Thornewell, P; Trent, P T; Tserkovnyak, Y I; Van Berg, R; Van de Water, R G; Virtue, C J; Waltham, C E; Wang, J X; Wark, D L; West, N; Wilhelmy, J B; Wilkerson, J F; Wilson, J; Wittich, P; Wouters, J M; Yeh, M

    2001-08-13

    Solar neutrinos from (8)B decay have been detected at the Sudbury Neutrino Observatory via the charged current (CC) reaction on deuterium and the elastic scattering (ES) of electrons. The flux of nu(e)'s is measured by the CC reaction rate to be straight phi(CC)(nu(e)) = 1.75 +/- 0.07(stat)(+0.12)(-0.11)(syst) +/- 0.05(theor) x 10(6) cm(-2) s(-1). Comparison of straight phi(CC)(nu(e)) to the Super-Kamiokande Collaboration's precision value of the flux inferred from the ES reaction yields a 3.3 sigma difference, assuming the systematic uncertainties are normally distributed, providing evidence of an active non- nu(e) component in the solar flux. The total flux of active 8B neutrinos is determined to be 5.44+/-0.99 x 10(6) cm(-2) s(-1).

  17. Radius studies of 8Li and 8B using the optical-limit Glauber model in conjunction with relativistic mean-field theory

    NASA Astrophysics Data System (ADS)

    Fan, Guang-Wei; Xu, Wang; Fukuda, M.; Pan, Qiang-Yan; Cai, Xiao-Lu; Fan, Gong-Tao; Li, Yong-Jiang; Luo, Wen; Xu, Ben-Ji; Yan, Zhe; Yang, Li-Feng

    2010-10-01

    We study the reaction cross sections (σR) and root-mean-square (RMS) radii of 8Li and 8B, the halo-like nuclei, with stable target 12C, 27Al and 9Be within the standard optical-limit Glauber model, using densities obtained from relativistic mean-field (RMF) formalisms and other types of distributions. It is found that the experimental σR can be reproduced well at high energy. The RMS radius and Δr extracted by RMF-theory and harmonic oscillator distribution are compared. We find that the RMS radius and Δr of 8B are larger than those of 8Li. In addition, we analyze in detail the relationship between σR and density distribution.

  18. Warm Spitzer Observations of Three Hot Exoplanets: XO-4b, HAT-P-6b, and HAT-P-8b

    NASA Astrophysics Data System (ADS)

    Todorov, Kamen O.; Deming, Drake; Knutson, Heather A.; Burrows, Adam; Sada, Pedro V.; Cowan, Nicolas B.; Agol, Eric; Desert, Jean-Michel; Fortney, Jonathan J.; Charbonneau, David; Laughlin, Gregory; Langton, Jonathan; Showman, Adam P.; Lewis, Nikole K.

    2012-02-01

    We analyze Warm Spitzer/Infrared Array Camera observations of the secondary eclipses of three planets, XO-4b, HAT-P-6b, and HAT-P-8b. We measure secondary eclipse amplitudes at 3.6 μm and 4.5 μm for each target. XO-4b exhibits a stronger eclipse depth at 4.5 μm than at 3.6 μm, which is consistent with the presence of a temperature inversion. HAT-P-8b shows a stronger eclipse amplitude at 3.6 μm and is best described by models without a temperature inversion. The eclipse depths of HAT-P-6b can be fitted with models with a small or no temperature inversion. We consider our results in the context of a postulated relationship between stellar activity and temperature inversion and a relationship between irradiation level and planet dayside temperature, as discussed by Knutson et al. and Cowan & Agol, respectively. Our results are consistent with these hypotheses, but do not significantly strengthen them. To measure accurate secondary eclipse central phases, we require accurate ephemerides. We obtain primary transit observations and supplement them with publicly available observations to update the orbital ephemerides of the three planets. Based on the secondary eclipse timing, we set upper boundaries for ecos (ω) for HAT-P-6b, HAT-P-8b, and XO-4b and find that the values are consistent with circular orbits.

  19. WARM SPITZER OBSERVATIONS OF THREE HOT EXOPLANETS: XO-4b, HAT-P-6b, AND HAT-P-8b

    SciTech Connect

    Todorov, Kamen O.; Deming, Drake; Knutson, Heather A.; Burrows, Adam; Sada, Pedro V.; Cowan, Nicolas B.; Agol, Eric; Desert, Jean-Michel; Charbonneau, David; Fortney, Jonathan J.; Laughlin, Gregory; Langton, Jonathan; Showman, Adam P.; Lewis, Nikole K.

    2012-02-10

    We analyze Warm Spitzer/Infrared Array Camera observations of the secondary eclipses of three planets, XO-4b, HAT-P-6b, and HAT-P-8b. We measure secondary eclipse amplitudes at 3.6 {mu}m and 4.5 {mu}m for each target. XO-4b exhibits a stronger eclipse depth at 4.5 {mu}m than at 3.6 {mu}m, which is consistent with the presence of a temperature inversion. HAT-P-8b shows a stronger eclipse amplitude at 3.6 {mu}m and is best described by models without a temperature inversion. The eclipse depths of HAT-P-6b can be fitted with models with a small or no temperature inversion. We consider our results in the context of a postulated relationship between stellar activity and temperature inversion and a relationship between irradiation level and planet dayside temperature, as discussed by Knutson et al. and Cowan and Agol, respectively. Our results are consistent with these hypotheses, but do not significantly strengthen them. To measure accurate secondary eclipse central phases, we require accurate ephemerides. We obtain primary transit observations and supplement them with publicly available observations to update the orbital ephemerides of the three planets. Based on the secondary eclipse timing, we set upper boundaries for ecos ({omega}) for HAT-P-6b, HAT-P-8b, and XO-4b and find that the values are consistent with circular orbits.

  20. Distinction between elastic scattering of weakly bound proton- and neutron-rich nuclei: The case of 8B and 11Be

    NASA Astrophysics Data System (ADS)

    Yang, Y. Y.; Liu, X.; Pang, D. Y.

    2016-09-01

    Experimental data show that the elastic scattering cross sections of the neutron-rich nucleus 11Be are greatly reduced by the coupling effects from the breakup channels, while those of the proton-rich nucleus 8B are not. Such difference is found to persist in results of systematic calculations of 8B elastic scattering from 208Pb at 60 and 170.3 MeV and from 64Zn at 32 and 86 MeV, and 11Be elastic scattering from 208Pb at 55 and 143 MeV and from 64Zn at 29 and 66 MeV with the continuum-discretized coupled channel (CDCC) method. The Coulomb and centrifugal barriers experienced by the valence proton in the ground state of 8B, which do not exist for the valence neutron in the ground state of 11Be, are found to be the reason for such differences in the angular distributions of elastic scattering cross sections of these two weakly bound nuclei.

  1. Improving a recombinant Zymomonas mobilis strain 8b through continuous adaptation on dilute acid pretreated corn stover hydrolysate

    SciTech Connect

    Mohagheghi, Ali; Linger, Jeffrey G.; Yang, Shihui; Smith, Holly; Dowe, Nancy; Zhang, Min; Pienkos, Philip T.

    2015-03-31

    Complete conversion of the major sugars of biomass including both the C5 and C6 sugars is critical for biofuel production processes. Several inhibitory compounds like acetate, hydroxymethylfurfural (HMF), and furfural are produced from the biomass pretreatment process leading to ‘hydrolysate toxicity,’ a major problem for microorganisms to achieve complete sugar utilization. Therefore, development of more robust microorganisms to utilize the sugars released from biomass under toxic environment is critical. In this study, we use continuous culture methodologies to evolve and adapt the ethanologenic bacterium Zymomonas mobilis to improve its ethanol productivity using corn stover hydrolysate. The results are the following: A turbidostat was used to adapt the Z. mobilis strain 8b in the pretreated corn stover liquor. The adaptation was initiated using pure sugar (glucose and xylose) followed by feeding neutralized liquor at different dilution rates. Once the turbidostat reached 60% liquor content, the cells began washing out and the adaptation was stopped. Several ‘sub-strains’ were isolated, and one of them, SS3 (sub-strain 3), had 59% higher xylose utilization than the parent strain 8b when evaluated on 55% neutralized PCS (pretreated corn stover) liquor. Using saccharified PCS slurry generated by enzymatic hydrolysis from 25% solids loading, SS3 generated an ethanol yield of 75.5% compared to 64% for parent strain 8b. Furthermore, the total xylose utilization was 57.7% for SS3 versus 27.4% for strain 8b. To determine the underlying genotypes in these new sub-strains, we conducted genomic resequencing and identified numerous single-nucleotide mutations (SNPs) that had arisen in SS3. We further performed quantitative reverse transcription PCR (qRT-PCR) on genes potentially affected by these SNPs and identified significant down-regulation of two genes, ZMO0153 and ZMO0776, in SS3 suggesting potential genetic mechanisms behind SS3

  2. Creep and Oxidation Behavior of Modified CF8C-Plus with W, Cu, Ni, and Cr

    NASA Astrophysics Data System (ADS)

    Unocic, Kinga A.; Dryepondt, Sebastien; Yamamoto, Yukinori; Maziasz, Philip J.

    2016-04-01

    The microstructures of modified CF8C-Plus (Fe-19Cr-12Ni-0.4W-3.8Mn-0.2Mo-0.6Nb-0.5Si-0.9C) with W and Cu (CF8CPWCu) and CF8CPWCu enhanced with 21Cr + 15Ni or 22Cr + 17.5Ni were characterized in the as-cast condition and after creep testing. When imaged at lower magnifications, the as-cast microstructure was similar among all three alloys as they all contained a Nb-rich interdendritic phase and Mn-based inclusions. Transmission electron microscopy (TEM) analysis showed the presence of nanoscale Cu-rich nanoprecipitates distributed uniformly throughout the matrix of CF8CPWCu, whereas in CF8CPWCu22/17, Cu precipitates were found primarily at the grain boundaries. The presence of these nanoscale Cu-rich particles, in addition to W-rich Cr23C6, nanoscale Nb carbides, and Z-phase (Nb2Cr2N2), improved the creep strength of the CF8CPWCu steel. Modification of CF8CPWCu with Cr and Ni contents slightly decreased the creep strength but significantly improved the oxidation behavior at 1073 K (800 °C). In particular, the addition of 22Cr and 17.5Ni strongly enhanced the oxidation resistance of the stainless steel resulting in a 100 degrees or greater temperature improvement, and this composition provided the best balance between improving both mechanical properties and oxidation resistance.

  3. Creep and oxidation behavior of modified CF8C-plus with W, Cu, Ni, and Cr

    DOE PAGES

    Unocic, Kinga A.; Dryepondt, Sebastien N.; Yamamoto, Yukinori; Maziasz, Philip J.

    2016-02-01

    Here, the microstructures of modified CF8C-Plus (Fe-19Cr-12Ni-0.4W-3.8Mn-0.2Mo-0.6Nb-0.5Si-0.9C) with W and Cu (CF8CPWCu) and CF8CPWCu enhanced with 21Cr + 15Ni or 22Cr + 17.5Ni were characterized in the as-cast condition and after creep testing. When imaged at lower magnifications, the as-cast microstructure was similar among all three alloys as they all contained a Nb-rich interdendritic phase and Mn-based inclusions. Transmission electron microscopy (TEM) analysis showed the presence of nanoscale Cu-rich nanoprecipitates distributed uniformly throughout the matrix of CF8CPWCu, whereas in CF8CPWCu22/17, Cu precipitates were found primarily at the grain boundaries. The presence of these nanoscale Cu-rich particles, in addition to W-richmore » Cr23C6, nanoscale Nb carbides, and Z-phase (Nb2Cr2N2), improved the creep strength of the CF8CPWCu steel. Modification of CF8CPWCu with Cr and Ni contents slightly decreased the creep strength but significantly improved the oxidation behavior at 1073 K (800 °C). In particular, the addition of 22Cr and 17.5Ni strongly enhanced the oxidation resistance of the stainless steel resulting in a 100 degrees or greater temperature improvement, and this composition provided the best balance between improving both mechanical properties and oxidation resistance.« less

  4. Optimized anisotropic magnetoelectric response of Fe61.6Co16.4Si10.8B11.2/PVDF/Fe61.6Co16.4Si10.8B11.2 laminates for AC/DC magnetic field sensing

    NASA Astrophysics Data System (ADS)

    Reis, S.; Silva, M. P.; Castro, N.; Correia, V.; Gutierrez, J.; Lasheras, A.; Lanceros-Mendez, S.; Martins, P.

    2016-05-01

    The anisotropic magnetoelectric (ME) effect on a Fe61.6Co16.4Si10.8B11.2/PVDF Fe61.6Co16.4Si10.8B11.2 laminate composite has been used for the development of a magnetic field sensor able to detect both the magnitude and direction of AC and DC magnetic fields. The accuracy (99% for both AC and DC sensors), linearity (92% for the DC sensor and 99% for the AC sensor) and reproducibility (99% for both sensors) indicate the suitability of the sensor for applications. Furthermore, the sensitivity of the Fe61.6Co16.4Si10.8B11.2/PVDF/Fe61.6Co16.4Si10.8B11.2 anisotropic magnetic sensor—15 and 1400 mV Oe-1 for the DC and AC fields, respectively—are the highest reported in the literature for polymer-based ME materials. Such features, combined with its flexibility, versatility, light weight, low cost and low-temperature fabrication, lead to the suitability of the developed sensor for use in magnetic sensor applications.

  5. Design and testing of new center inlet and S-duct for B-727 airplane with refanned JT8D engines

    NASA Technical Reports Server (NTRS)

    Ting, C. T.; Kaldschmidt, G.; Syltebo, B. E.

    1975-01-01

    The work described in this paper was part of the NASA refan program. The airflow requirement of the refanned JT8D (-100 series) engine increased about 50% above that of the basic JT8D. This required a redesign of the center inlet and S-duct of the Boeing 727 airplane. The paper describes the design constraints for the S-duct and the analytic method used to define the lines of the new duct. Model tests that were conducted at static, angle-of-attack, and crosswind conditions are described with a variety of flow control devices. Test results showed that the new inlet and S-duct have a pressure recovery comparable to that of the existing inlet. By employing corotating vortex generators, less flow distortion was obtained for the core region than the existing duct has with its counter rotating vortex generators.

  6. Some comparisons of the flyover noise characteristics of DC-9 aircraft having refanned and hardwalled JT8D engines, with special reference to measurement and analysis procedures

    NASA Technical Reports Server (NTRS)

    Hosier, R. N.

    1976-01-01

    Flyover noise measurements were made (using Federal Aviation Regulations, part 36 procedures) of two DC-9 aircraft, one equipped with refanned JT8D-109 engines and the other equipped with hardwalled JT8D-9 engines. NASA analyses show a refan centerline noise reduction of about 9.1 EPNdB and 10.0 EPNdB for takeoff with cutback and 50 deg. flap landing approach, respectively. A comparison of refan and hardwall PNLTM spectra shows that the refan noise reduction may be attributed to lower jet noise levels on takeoff and reduced high-frequency tonal content on landing approach. A general description of the test procedures and results are included along with detailed descriptions of the measurement and analysis systems.

  7. Warm Spitzer Secondary Transit Photometry of Hot Jupiters HAT-P-6b, HAT-P-8b and XO-4b

    NASA Astrophysics Data System (ADS)

    Todorov, Kamen 0.; Deming, D.; Knutson, H.; Burrows, A.; Sada, P.; Agol, E.; Desert, J.; Fortney, J. J.; Charbonneau, D.; Cowan, N. B.; Laughlin, G.; Langton, J.; Showman, A. P.; Lewis, N. K.

    2011-05-01

    An increasing number of transiting exoplanets have been observed at secondary eclipse. By measuring the depth of these eclipses at different wavelengths it is possible to distinguish between planets that have a temperature inversion in the upper layers of their atmospheres and ones that do not. We observed XO-4b, HAT-P-6b and HAT-P-8b during secondary eclipse with the IRAC instrument on Warm Spitzer at 3.6 and 4.5 microns. We compare the resulting eclipse depths to atmospheric models with and without temperature inversions, and thereby place constraints on the properties of their day-side atmospheres and heat redistribution efficiencies. The XO-4b and HAT-P-6b eclipse depths agree best with inverted models, while HAT-P-8b exhibits no temperature inversion. Knutson et al. (2010) hypothesized a correlation between lack of a temperature inversion and host star activity. Also, Cowan & Agol (2011), investigated the dependence between planetary effective temperatures, assuming no redistribution, and heat redistribution efficiency, finding that the hottest planets re-distribute heat inefficiently. We compare our planets with the Knutson and Cowan-Agol relations, and we find that they are consistent with the Knutson et al. activity hypothesis, but they are not hot enough to test the Cowan & Agol hypothesis.

  8. Corrosion Behaviour of Ni{sub 78}Si{sub 8}B{sub 14} Alloy in 1 M NaCl Aqueous Medium at Room Temperature

    SciTech Connect

    Mathur, Shubhra; Vyas, Rishi; Sachdev, K.; Sharma, S. K.

    2010-06-29

    Potentiodynamic polarization studies were performed on the air side and wheel side of the specimen Ni{sub 78}Si{sub 8}B{sub 14} in 1 M NaCl aqueous medium at room temperature. It was observed that the corrosion current density on the air side of the specimen was less than that of the wheel side of the specimen in 1 M NaCl aqueous medium. The amorphous nature of the air side and wheel side of the specimen Ni{sub 78}Si{sub 8}B{sub 14} was confirmed by X-ray diffraction (XRD). The observed behaviour can be related to the surface morphology due to processing of the ribbons. It is suggested that the inferior corrosion resistance of the wheel side surface is attributed to higher concentration of quenched-in defects due to higher cooling rate. Weight loss studies were also carried out in order to corroborate the polarization results. The results are discussed in the paper in the light of data reported in the literature.

  9. Measurement of the nue and Total 8B Solar Neutrino Fluxes with theSudbury Neutrino Observatory Phase I Data Set

    SciTech Connect

    Aharmim, B.; Ahmad, Q.R.; Ahmed, S.N.; Allen, R.C.; Andersen,T.C.; Anglin, J.D.; Buehler, G.; Barton, J.C.; Beier, E.W.; Bercovitch,M.; Bergevin, M.; Bigu, J.; Biller, S.D.; Black, R.A.; Blevis, I.; Boardman, R.J.; Boger, J.; Bonvin, E.; Boulay, M.G.; Bowler, M.G.; Bowles, T.J.; Brice, S.J.; Browne, M.C.; Bullard, T.V.; Burritt, T.H.; Cameron, J.; Chan, Y.D.; Chen, H.H.; Chen, M.; Chen, X.; Cleveland, B.T.; Cowan, J.H.M.; Cowen, D.F.; Cox, G.A.; Currat, C.A.; Dai, X.; Dalnoki-Veress, F.; Davidson, W.F.; Deng, H.; DiMarco, M.; Doe, P.J.; Doucas, G.; Dragowsky, M.R.; Duba, C.A.; Duncan, F.A.; Dunford, M.; Dunmore, J.A.; Earle, E.D.; Elliott, S.R.; Evans, H.C.; Ewan, G.T.; Farine, J.; Fergani, H.; Ferraris, A.P.; Fleurot, F.; Ford, R.J.; Formaggio, J.A.; Fowler, M.M.; Frame, K.; Frank, E.D.; Frati, W.; Gagnon,N.; Germani, J.V.; Gil, S.; Goldschmidt, A.; Goon, J.T.M.; Graham, K.; Grant, D.R.; Guillian, E.; Hahn, R.L.; Hallin, A.L.; Hallman, E.D.; Hamer, A.S.; Hamian, A.A.; Handler, W.B.; Haq, R.U.; Hargrove, C.K.; Harvey, P.J.; Hazama, R.; Heeger, K.M.; Heintzelman, W.J.; Heise, J.; Helmer, R.L.; Henning, R.; Hepburn, J.D.; Heron, H.; Hewett, J.; Hime,A.; Howard, C.; Howe, M.A.; Huang, M.; Hykawy, J.G.; Isaac, M.C.P.; Jagam, P.; Jamieson, B.; Jelley, N.A.; Jillings, C.; Jonkmans, G.; Kazkaz, K.; Keener, P.T.; Kirch, K.; Klein, J.R.; Knox, A.B.; Komar,R.J.; Kormos, L.L.; Kos, M.; Kouzes, R.; Krueger, A.; Kraus, C.; Krauss,C.B.; Kutter, T.; Kyba, C.C.M.; Labranche, H.; Lange, R.; Law, J.; Lawson, I.T.; Lay, M.; Lee, H.W.; Lesko, K.T.; Leslie, J.R.; Levine, I.; Loach, J.C.; Locke, W.; Luoma, S.; Lyon, J.; MacLellan, R.; Majerus, S.; Mak, H.B.; Maneira, J.; Marino, A.D.; Martin, R.; McCauley, N.; McDonald,A.B.; McDonald, D.S.; McFarlane, K.; McGee, S.; McGregor, G.; MeijerDrees, R.; Mes, H.; Mifflin, C.; Miknaitis, K.K.S.; Miller, M.L.; Milton,G.; Moffat, B.A.; Monreal, B.; Moorhead, M.; Morrissette, B.; Nally,C.W.; Neubauer, M.S.; et al.

    2007-02-01

    This article provides the complete description of resultsfrom the Phase I data set of the Sudbury Neutrino Observatory (SNO). ThePhase I data set is based on a 0.65 kt-year exposure of heavy water tothe solar 8B neutrino flux. Included here are details of the SNO physicsand detector model, evaluations of systematic uncertainties, andestimates of backgrounds. Also discussed are SNO's approach tostatistical extraction of the signals from the three neutrino reactions(charged current, neutral current, and elastic scattering) and theresults of a search for a day-night asymmetry in the ?e flux. Under theassumption that the 8B spectrum is undistorted, the measurements fromthis phase yield a solar ?e flux of ?(?e) =1.76+0.05?0.05(stat.)+0.09?0.09 (syst.) x 106 cm?2 s?1, and a non-?ecomponent ?(? mu) = 3.41+0.45?0.45(stat.)+0.48?0.45 (syst.) x 106 cm?2s?1. The sum of these components provides a total flux in excellentagreement with the predictions of Standard Solar Models. The day-nightasymmetry in the ?e flux is found to be Ae = 7.0 +- 4.9 (stat.)+1.3?1.2percent (sys.), when the asymmetry in the total flux is constrained to bezero.

  10. Identification of a key recombinant narrows the CADASIL gene region to 8 cM and argues against allelism of CADASIL and familial hemiplegic migraine

    SciTech Connect

    Dichgans, M.; Mayer, M.; Straube, A.

    1996-02-15

    This article reports on new information regarding the genetic mapping of the human CADASIL gene region. Previously, the gene had been mapped to human chromosome 19q12. Using the identification of a chromosomal crossover, the region has been refined to an 8-cM interval. 11 refs., 2 figs., 1 tab.

  11. 26 CFR 31.3401(a)(8)(C)-1 - Remuneration for services performed in Puerto Rico by citizen of the United States.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 15 2010-04-01 2010-04-01 false Remuneration for services performed in Puerto... Source § 31.3401(a)(8)(C)-1 Remuneration for services performed in Puerto Rico by citizen of the United States. (a) Remuneration paid for services performed within Puerto Rico for an employer (other than...

  12. Ground and flight test experience with a triple redundant digital fly by wire control system. [installed in F-8C aircraft

    NASA Technical Reports Server (NTRS)

    Jarvis, C. R.; Szalai, K. J.

    1981-01-01

    A triplex digital fly by wire flight control system was developed and installed in an F-8C aircraft to provide fail operative, full authority control. Hardware and software redundancy management techniques were designed to detect and identify failures in the system. Control functions typical of those projected for future actively controlled vehicles were implemented.

  13. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  14. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  15. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  16. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  17. 40 CFR 180.1250 - C8, C10, and C12 fatty acid monoesters of glycerol and propylene glycol; exemption from the...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Exemptions From Tolerances § 180.1250 C8... a tolerance. The C8, C10, and C12 straight-chain fatty acid monoesters of glycerol (glycerol... of a tolerance in or on all food commodities when used in accordance with approved label rates...

  18. Antimicrobial and anti-biofilm effect of Bac8c on major bacteria associated with dental caries and Streptococcus mutans biofilms.

    PubMed

    Ding, Yonglin; Wang, Wei; Fan, Meng; Tong, Zhongchun; Kuang, Rong; Jiang, WenKai; Ni, Longxing

    2014-02-01

    Dental caries is a common oral bacterial infectious disease. Its prevention and treatment requires control of the causative pathogens within dental plaque, especially Streptococcus mutans (S. mutans). Antimicrobial peptides (AMPs), one of the promising substitutes for conventional antibiotics, have been widely tested and used for controlling bacterial infections. The present study focuses on evaluating the potential of the novel AMPs cyclic bactenecin and its derivatives against bacteria associated with dental caries. The results indicate that Bac8c displayed highest activity against the bacteria tested, whereas both cyclic and linear bactenecin had weak antimicrobial activity. The cytotoxicity assay showed that Bac8c did not cause detectable toxicity at concentrations of 32-128μg/ml for 5min or 32-64μg/ml for 60min. S. mutans and Lactobacillus fermenti treated with Bac8c showed variable effects on bacterial structure via scanning electron microscopy and transmission electron microscopy. There appeared to be a large amount of extracellular debris and obvious holes on the cell surface, as well as loss of cell wall and nucleoid condensation. The BioFlux system was employed to generate S. mutans biofilms under a controlled flow, which more closely resemble the formation process of natural biofilms. Bac8c remarkably reduced the viability of cells in biofilms formed in the BioFlux system. This phenomenon was further analyzed and verified by real-time PCR results of a significant suppression of the genes involved in S. mutans biofilm formation. Taken together, this study suggests that Bac8c has a potential clinical application in preventing and treating dental caries.

  19. Synthesis, structural elucidation and spectroscopic analysis of 3a,8b-dihydroxy-4-oxo-1H,2H,3H,3aH,4H,8bH-indeno[1,2-d]imidazolidin-2-iminium chloride

    NASA Astrophysics Data System (ADS)

    Uma Devi, T.; Priya, S.; Selvanayagam, S.; Ravikumar, K.; Anitha, K.

    2012-11-01

    Ninhydrin guanidinium chloride (3a,8b-dihydroxy-4-oxo-1H,2H,3H,3aH,4H,8bH-indeno [1,2-d]imidazolidin-2-iminium chloride) a semiorganic crystal was synthesized. The structure was determined using X-ray single crystal technique. Comparisons between the FT-IR spectrum of ninhydrin guanidinium chloride with ninhydrin were made. Melting point was found using thermal measurements. The molecular geometry, vibrational frequencies and Mulliken charges of the compound in the ground state have been calculated by the density functional theory (DFT) method with 3-21G(d,p) basis set and theoretical frequencies were compared with the experimental FT-IR spectrum. Besides, molecular electrostatic potential (MEP), frontier molecular orbitals (FMO) analysis, natural bond orbitals (NBO) and thermodynamic properties at various temperatures of the compound were investigated by theoretical calculations.

  20. Independent measurement of the total active 8B solar neutrino flux using an array of 3He proportional counters at the Sudbury Neutrino Observatory.

    PubMed

    Aharmim, B; Ahmed, S N; Amsbaugh, J F; Anthony, A E; Banar, J; Barros, N; Beier, E W; Bellerive, A; Beltran, B; Bergevin, M; Biller, S D; Boudjemline, K; Boulay, M G; Bowles, T J; Browne, M C; Bullard, T V; Burritt, T H; Cai, B; Chan, Y D; Chauhan, D; Chen, M; Cleveland, B T; Cox-Mobrand, G A; Currat, C A; Dai, X; Deng, H; Detwiler, J; DiMarco, M; Doe, P J; Doucas, G; Drouin, P-L; Duba, C A; Duncan, F A; Dunford, M; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Ford, R J; Formaggio, J A; Fowler, M M; Gagnon, N; Germani, J V; Goldschmidt, A; Goon, J T M; Graham, K; Guillian, E; Habib, S; Hahn, R L; Hallin, A L; Hallman, E D; Hamian, A A; Harper, G C; Harvey, P J; Hazama, R; Heeger, K M; Heintzelman, W J; Heise, J; Helmer, R L; Henning, R; Hime, A; Howard, C; Howe, M A; Huang, M; Jagam, P; Jamieson, B; Jelley, N A; Keeter, K J; Klein, J R; Kormos, L L; Kos, M; Krüger, A; Kraus, C; Krauss, C B; Kutter, T; Kyba, C C M; Lange, R; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Loach, J C; MacLellan, R; Majerus, S; Mak, H B; Maneira, J; Martin, R; McBryde, K; McCauley, N; McDonald, A B; McGee, S; Mifflin, C; Miller, G G; Miller, M L; Monreal, B; Monroe, J; Morissette, B; Myers, A; Nickel, B G; Noble, A J; Oblath, N S; O'Keeffe, H M; Ollerhead, R W; Gann, G D Orebi; Oser, S M; Ott, R A; Peeters, S J M; Poon, A W P; Prior, G; Reitzner, S D; Rielage, K; Robertson, B C; Robertson, R G H; Rollin, E; Schwendener, M H; Secrest, J A; Seibert, S R; Simard, O; Simpson, J J; Sinclair, L; Skensved, P; Smith, M W E; Steiger, T D; Stonehill, L C; Tesić, G; Thornewell, P M; Tolich, N; Tsui, T; Tunnell, C D; Van Wechel, T; Van Berg, R; VanDevender, B A; Virtue, C J; Walker, T J; Wall, B L; Waller, D; Tseung, H Wan Chan; Wendland, J; West, N; Wilhelmy, J B; Wilkerson, J F; Wilson, J R; Wouters, J M; Wright, A; Yeh, M; Zhang, F; Zuber, K

    2008-09-12

    The Sudbury Neutrino Observatory (SNO) used an array of 3He proportional counters to measure the rate of neutral-current interactions in heavy water and precisely determined the total active (nu_x) 8B solar neutrino flux. This technique is independent of previous methods employed by SNO. The total flux is found to be 5.54_-0.31;+0.33(stat)-0.34+0.36(syst)x10(6) cm(-2) s(-1), in agreement with previous measurements and standard solar models. A global analysis of solar and reactor neutrino results yields Deltam2=7.59_-0.21;+0.19x10(-5) eV2 and theta=34.4_-1.2;+1.3 degrees. The uncertainty on the mixing angle has been reduced from SNO's previous results.

  1. Measurement of the νe and total 8B solar neutrino fluxes with the Sudbury Neutrino Observatory phase-III data set

    NASA Astrophysics Data System (ADS)

    Aharmim, B.; Ahmed, S. N.; Amsbaugh, J. F.; Anaya, J. M.; Anthony, A. E.; Banar, J.; Barros, N.; Beier, E. W.; Bellerive, A.; Beltran, B.; Bergevin, M.; Biller, S. D.; Boudjemline, K.; Boulay, M. G.; Bowles, T. J.; Browne, M. C.; Bullard, T. V.; Burritt, T. H.; Cai, B.; Chan, Y. D.; Chauhan, D.; Chen, M.; Cleveland, B. T.; Cox, G. A.; Currat, C. A.; Dai, X.; Deng, H.; Detwiler, J. A.; DiMarco, M.; Doe, P. J.; Doucas, G.; Dragowsky, M. R.; Drouin, P.-L.; Duba, C. A.; Duncan, F. A.; Dunford, M.; Earle, E. D.; Elliott, S. R.; Evans, H. C.; Ewan, G. T.; Farine, J.; Fergani, H.; Fleurot, F.; Ford, R. J.; Formaggio, J. A.; Fowler, M. M.; Gagnon, N.; Germani, J. V.; Goldschmidt, A.; Goon, J. TM.; Graham, K.; Guillian, E.; Habib, S.; Hahn, R. L.; Hallin, A. L.; Hallman, E. D.; Hamian, A. A.; Harper, G. C.; Harvey, P. J.; Hazama, R.; Heeger, K. M.; Heintzelman, W. J.; Heise, J.; Helmer, R. L.; Henning, R.; Hime, A.; Howard, C.; Howe, M. A.; Huang, M.; Jagam, P.; Jamieson, B.; Jelley, N. A.; Keeter, K. J.; Klein, J. R.; Kormos, L. L.; Kos, M.; Krüger, A.; Kraus, C.; Krauss, C. B.; Kutter, T.; Kyba, C. C. M.; Lange, R.; Law, J.; Lawson, I. T.; Lesko, K. T.; Leslie, J. R.; Loach, J. C.; MacLellan, R.; Majerus, S.; Mak, H. B.; Maneira, J.; Martin, R.; McCauley, N.; McDonald, A. B.; McGee, S. R.; Mifflin, C.; Miller, G. G.; Miller, M. L.; Monreal, B.; Monroe, J.; Morissette, B.; Myers, A. W.; Nickel, B. G.; Noble, A. J.; O'Keeffe, H. M.; Oblath, N. S.; Ollerhead, R. W.; Orebi Gann, G. D.; Oser, S. M.; Ott, R. A.; Peeters, S. J. M.; Poon, A. W. P.; Prior, G.; Reitzner, S. D.; Rielage, K.; Robertson, B. C.; Robertson, R. G. H.; Rollin, E.; Schwendener, M. H.; Secrest, J. A.; Seibert, S. R.; Simard, O.; Simpson, J. J.; Skensved, P.; Smith, M. W. E.; Sonley, T. J.; Steiger, T. D.; Stonehill, L. C.; Tešić, G.; Thornewell, P. M.; Tolich, N.; Tsui, T.; Tunnell, C. D.; Van Wechel, T.; Van Berg, R.; VanDevender, B. A.; Virtue, C. J.; Wall, B. L.; Waller, D.; Wan Chan Tseung, H.; Wendland, J.; West, N.; Wilhelmy, J. B.; Wilkerson, J. F.; Wilson, J. R.; Wouters, J. M.; Wright, A.; Yeh, M.; Zhang, F.; Zuber, K.

    2013-01-01

    This paper details the solar neutrino analysis of the 385.17-day phase-III data set acquired by the Sudbury Neutrino Observatory (SNO). An array of 3He proportional counters was installed in the heavy-water target to measure precisely the rate of neutrino-deuteron neutral-current interactions. This technique to determine the total active 8B solar neutrino flux was largely independent of the methods employed in previous phases. The total flux of active neutrinos was measured to be 5.54-0.31+0.33(stat.)-0.34+0.36(syst.)×106 cm-2 s-1, consistent with previous measurements and standard solar models. A global analysis of solar and reactor neutrino mixing parameters yielded the best-fit values of Δm2=7.59-0.21+0.19×10-5eV2 and θ=34.4-1.2+1.3degrees.

  2. Navier-Stokes simulation of external/internal transonic flow on the forebody/inlet of the AV-8B Harrier II

    NASA Technical Reports Server (NTRS)

    Mysko, Stephen J.; Chyu, Wei J.; Stortz, Michael W.; Chow, Chuen-Yen

    1993-01-01

    In this work, the computation of combined external/internal transonic flow on the complex forebody/inlet configuration of the AV-8B Harrier II is performed. The actual aircraft has been measured and its surface and surrounding domain, in which the fuselage and inlet have a common wall, have been described using structured grids. The 'thin-layer' Navier-Stokes equations were used to model the flow along with the Chimera embedded multi-block technique. A fully conservative, alternating direction implicit (ADI), approximately factored, partially fluxsplit algorithm was employed to perform the computation. Comparisons to some experimental wind tunnel data yielded good agreement for flow at zero incidence and angle of attack. The aim of this paper is to provide a methodology or computational tool for the numerical solution of complex external/internal flows.

  3. Independent measurement of the total active 8B solar neutrino flux using an array of 3He proportional counters at the Sudbury Neutrino Observatory.

    PubMed

    Aharmim, B; Ahmed, S N; Amsbaugh, J F; Anthony, A E; Banar, J; Barros, N; Beier, E W; Bellerive, A; Beltran, B; Bergevin, M; Biller, S D; Boudjemline, K; Boulay, M G; Bowles, T J; Browne, M C; Bullard, T V; Burritt, T H; Cai, B; Chan, Y D; Chauhan, D; Chen, M; Cleveland, B T; Cox-Mobrand, G A; Currat, C A; Dai, X; Deng, H; Detwiler, J; DiMarco, M; Doe, P J; Doucas, G; Drouin, P-L; Duba, C A; Duncan, F A; Dunford, M; Earle, E D; Elliott, S R; Evans, H C; Ewan, G T; Farine, J; Fergani, H; Fleurot, F; Ford, R J; Formaggio, J A; Fowler, M M; Gagnon, N; Germani, J V; Goldschmidt, A; Goon, J T M; Graham, K; Guillian, E; Habib, S; Hahn, R L; Hallin, A L; Hallman, E D; Hamian, A A; Harper, G C; Harvey, P J; Hazama, R; Heeger, K M; Heintzelman, W J; Heise, J; Helmer, R L; Henning, R; Hime, A; Howard, C; Howe, M A; Huang, M; Jagam, P; Jamieson, B; Jelley, N A; Keeter, K J; Klein, J R; Kormos, L L; Kos, M; Krüger, A; Kraus, C; Krauss, C B; Kutter, T; Kyba, C C M; Lange, R; Law, J; Lawson, I T; Lesko, K T; Leslie, J R; Loach, J C; MacLellan, R; Majerus, S; Mak, H B; Maneira, J; Martin, R; McBryde, K; McCauley, N; McDonald, A B; McGee, S; Mifflin, C; Miller, G G; Miller, M L; Monreal, B; Monroe, J; Morissette, B; Myers, A; Nickel, B G; Noble, A J; Oblath, N S; O'Keeffe, H M; Ollerhead, R W; Gann, G D Orebi; Oser, S M; Ott, R A; Peeters, S J M; Poon, A W P; Prior, G; Reitzner, S D; Rielage, K; Robertson, B C; Robertson, R G H; Rollin, E; Schwendener, M H; Secrest, J A; Seibert, S R; Simard, O; Simpson, J J; Sinclair, L; Skensved, P; Smith, M W E; Steiger, T D; Stonehill, L C; Tesić, G; Thornewell, P M; Tolich, N; Tsui, T; Tunnell, C D; Van Wechel, T; Van Berg, R; VanDevender, B A; Virtue, C J; Walker, T J; Wall, B L; Waller, D; Tseung, H Wan Chan; Wendland, J; West, N; Wilhelmy, J B; Wilkerson, J F; Wilson, J R; Wouters, J M; Wright, A; Yeh, M; Zhang, F; Zuber, K

    2008-09-12

    The Sudbury Neutrino Observatory (SNO) used an array of 3He proportional counters to measure the rate of neutral-current interactions in heavy water and precisely determined the total active (nu_x) 8B solar neutrino flux. This technique is independent of previous methods employed by SNO. The total flux is found to be 5.54_-0.31;+0.33(stat)-0.34+0.36(syst)x10(6) cm(-2) s(-1), in agreement with previous measurements and standard solar models. A global analysis of solar and reactor neutrino results yields Deltam2=7.59_-0.21;+0.19x10(-5) eV2 and theta=34.4_-1.2;+1.3 degrees. The uncertainty on the mixing angle has been reduced from SNO's previous results. PMID:18851271

  4. Inclusion body hepatitis associated with an outbreak of fowl adenovirus type 2 and type 8b in broiler flocks in South Africa.

    PubMed

    Maartens, Louis H; Joubert, Hilda W; Aitchison, Henry; Venter, Estelle H

    2015-01-01

    Inclusion body hepatitis is an acute disease of chickens ascribed to viruses of the genus Aviadenovirus and referred to as fowl adenovirus (FAdV). There are 12 FAdV types (FAdV1to FAdV8a and FAdV8b to FAdV11), classified into five species based on their genotype (designated FAdVA to FAdVE). A total of 218 000 chickens, 2-29 days of age, were affected over a 1-year period, all testing positive by microscopy, virus isolation and confirmation with polymerase chain reaction (PCR). Affected birds were depressed, lost body weight,were weak and had watery droppings. Pathological changes observed during necropsy indicated consistent changes in the liver, characterised by hepatomegaly, cholestasis and hepatitis. Lesions were also discernible in the spleen, kidney and gizzard wall and were characterised by splenomegaly, pinpoint haemorrhages, nephritis with haemorrhage,visceral gout and serosal ecchymosis of the gizzard wall. Histopathological lesions were most consistently observed in the liver but could also be seen in renal and splenic tissue. Virus isolation was achieved in embryonated eggs and most embryos revealed multifocalto diffuse hepatic necrosis, with a mixed cellular infiltrate of macrophages and heterophils(necro-granulomas), even in the absence of macroscopic pathology. Virus isolation results were verified by histopathology and PCR on embryonic material and further characterised by nucleotide sequence analysis. Two infectious bursal disease virus isolates were also made from the Klerksdorp flock. Nucleotide sequence analysis of the L1 hexon loop of all the FAdV isolates indicated homology (99%) with prototype strains P7-A for FAdV-2, as well as for FAdV-8b.

  5. Phase 2 program on ground test of refanned JT8D turbofan engines and nacelles for the 727 airplane. Volume 1: Summary

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The propulsion performance, acoustic, structural, and systems changes to a 727-200 airplane retrofitted with a refan modification of the JT8D turbofan engine are evaluated. Model tests, design of certifiable airplane retrofit kit hardware, manufacture of test hardware, ground test of a current production JT8D engine, followed by test of the same engine modified to the refan configuration, detailed analyses of the retrofit impact on airplane airworthiness, performance, and noise, and a preliminary analysis of retrofit costs are included. Results indicate that the refan retrofit of the 727-200 would be certifiable and would result in a 6-to 8 EPNdb reduction in effective perceived noise level (EPNL) at the FAR 36 measuring points and an annoyance-weighted footprint area reduction of 68% to 83%. The installed refan engine is estimated to provide 14% greater takeoff thrust at zero velocity and 10% greater thrust at 100 kn (51.4 m/s). There would be an approximate 0.6% increase in cruise specific fuel consumption (SFC). The refan engine performance in conjunction with the increase in stalled weight results in a range reduction of approximately 15% over the unmodified airplane at the same brake release gross weight (BRGW), with a block fuel increase of 1.5% to 3%. With the particular model 727 that was studied, however, it is possible to operate the airplane (with minor structural modifications) at a higher BRGW and increase the range up to approximately 15% relative to the nonrefanned airplane (with equal or slightly increased noise levels). The JT8D refan engine also improves the limited-field range of the airplane.

  6. Cruise Drag Results from High Speed Wind Tunnel Tests of NASA Refan JT8D Engine Nacelles on the Boeing 727-200

    NASA Technical Reports Server (NTRS)

    Easterbrook, W. G.; Carlson, R. B.

    1973-01-01

    High speed wind tunnel test results are presented showing the cruise drag effect of installing JT8D-109 Refan engines on a Boeing 727-200. Incremental drags of a refan center inlet and side nacelles are presented for several configuration variations. Static pressure distributions were obtained on the side nacelle strut and on the fuselage (above and below the strut). Oil flow photographs of selected configurations are also presented. In general the drag level of the refan installation is slightly better than predicted prior to the test and the drag rise is favorable.

  7. Knockdown of ATP8B1 expression leads to specific downregulation of the bile acid sensor FXR in HepG2 cells: effect of the FXR agonist GW4064.

    PubMed

    Martínez-Fernández, Pilar; Hierro, Loreto; Jara, Paloma; Alvarez, Luis

    2009-05-01

    Farnesoid X receptor (FXR) is a bile acid-sensing nuclear receptor that controls bile acid homeostasis. It has been suggested that downregulation of FXR contributes to the pathogenesis of an inherited disorder of bile secretion caused by mutations in ATP8B1. We have investigated the relationship between ATP8B1 knockdown and FXR downregulation in the human hepatoblastoma cell line HepG2. Transfection of HepG2 cells with ATP8B1 small interfering RNA (siRNA) duplexes led to a 60% reduction in the endogenous levels of ATP8B1 mRNA and protein and a concomitant decrease in FXR mRNA and protein content, as well as in FXR phosphorylation. This decrease was accompanied by a marked reduction in mRNA levels of a subset of FXR targets, such as bile salt export pump (ABCB11), small heterodimer partner, and uridine 5'-diphosphate-glucuronosyltransferase. ATP8B1 inhibition specifically targeted FXR since mRNA expression of other prominent nuclear receptors, such as pregnane X receptor and constitutive androstane receptor, or liver-enriched transcription factors, such as hepatocyte nuclear factor 1alpha (HNF-1alpha) and HNF-4alpha, was not altered. The expression of other key genes involved in bile acid synthesis, detoxification, and transport also remained unchanged upon ATP8B1 knockdown. Supporting the specificity of the effect, siRNA-mediated silencing of ABCB11, whose defect is associated with another inherited disorder of bile secretion, did not affect FXR expression. Treatment with the synthetic FXR agonist GW4064 was able to partially neutralize ATP8B1 siRNA-mediated FXR downregulation and fully counteract inhibition of FXR target genes. Collectively these findings indicate that ATP8B1 knockdown specifically downregulates FXR, and this action can be circumvented by treatment with FXR agonists.

  8. A functional N-terminal domain in C/EBPβ-LAP* is required for interacting with SWI/SNF and to repress Ric-8B gene transcription in osteoblasts.

    PubMed

    Aguilar, Rodrigo; Grandy, Rodrigo; Meza, Daniel; Sepulveda, Hugo; Pihan, Philippe; van Wijnen, Andre J; Lian, Jane B; Stein, Gary S; Stein, Janet L; Montecino, Martin

    2014-10-01

    The chromatin remodeling complex SWI/SNF and the transcription factor C/EBPβ play critical roles in osteoblastic cells as they jointly control transcription of a number of bone-related target genes. The largest C/EBPβ isoform, LAP*, possesses a short additional N-terminal domain that has been proposed to mediate the interaction of this factor with SWI/SNF in myeloid cells. Here we examine the requirement of a functional N-terminus in C/EBPβ-LAP* for binding SWI/SNF and for recruiting this complex to the Ric-8B gene to mediate transcriptional repression. We find that both C/EBPβ-LAP* and SWI/SNF simultaneously bind to the Ric-8B promoter in differentiating osteoblasts that repress Ric-8B expression. This decreased expression of Ric-8B is not accompanied by significant changes in histone acetylation at the Ric-8B gene promoter sequence. A single aminoacid change at the C/EBPβ-LAP* N-terminus (R3L) that inhibits C/EBPβ-LAP*-SWI/SNF interaction, also prevents SWI/SNF recruitment to the Ric-8B promoter as well as C/EBPβ-LAP*-dependent repression of the Ric-8B gene. Inducible expression of the C/EBPβ-LAP*R3L protein in stably transfected osteoblastic cells demonstrates that this mutant protein binds to C/EBPβ-LAP*-target promoters and competes with the endogenous C/EBPβ factor. Together our results indicate that a functional N-terminus in C/EBPβ-LAP* is required for interacting with SWI/SNF and for Ric-8B gene repression in osteoblasts.

  9. An Independent Measurement of the Total Active 8B Solar Neutrino Flux Using an Array of 3He Proportional Counters at the Sudbury Neutrino Observatory

    SciTech Connect

    SNO Colla

    2008-06-05

    The Sudbury Neutrino Observatory (SNO) used an array of {sup 3}He proportional counters to measure the rate of neutral-current interactions in heavy water and precisely determined the total active ({nu}{sub x}) {sup 8}B solar neutrino flux. This technique is independent of previous methods employed by SNO. The total flux is found to be 5.54{sub -0.31}{sup +0.33}(stat){sub -0.34}{sup +0.36}(syst) x 10{sup 6} cm{sup -2}s{sup -1}, in agreement with previous measurements and standard solar models. A global analysis of solar and reactor neutrino results yields {Delta}m{sup 2} = 7.94{sub -0.26}{sup +0.42} x 10{sup -5} eV{sup 2} and {theta} = 33.8{sub -1.3}{sup +1.4} degrees. The uncertainty on the mixing angle has been reduced from SNO's previous results.

  10. The lipid flippase heterodimer ATP8B1-CDC50A is essential for surface expression of the apical sodium-dependent bile acid transporter (SLC10A2/ASBT) in intestinal Caco-2 cells.

    PubMed

    van der Mark, Vincent A; de Waart, D Rudi; Ho-Mok, Kam S; Tabbers, Merit M; Voogt, Heleen W; Oude Elferink, Ronald P J; Knisely, A S; Paulusma, Coen C

    2014-12-01

    Deficiency of the phospholipid flippase ATPase, aminophospholipid transporter, class I, type 8B, member 1 (ATP8B1) causes progressive familial intrahepatic cholestasis type 1 (PFIC1) and benign recurrent intrahepatic cholestasis type 1 (BRIC1). Apart from cholestasis, many patients also suffer from diarrhea of yet unknown etiology. Here we have studied the hypothesis that intestinal ATP8B1 deficiency results in bile salt malabsorption as a possible cause of PFIC1/BRIC1 diarrhea. Bile salt transport was studied in ATP8B1-depleted intestinal Caco-2 cells. Apical membrane localization was studied by a biotinylation approach. Fecal bile salt and electrolyte contents were analyzed in stool samples of PFIC1 patients, of whom some had undergone biliary diversion or liver transplantation. Bile salt uptake by the apical sodium-dependent bile salt transporter solute carrier family 10 (sodium/bile acid cotransporter), member 2 (SLC10A2) was strongly impaired in ATP8B1-depleted Caco-2 cells. The reduced SLC10A2 activity coincided with strongly reduced apical membrane localization, which was caused by impaired apical membrane insertion of SLC10A2. Moreover, we show that endogenous ATP8B1 exists in a functional heterodimer with transmembrane protein 30A (CDC50A) in Caco-2 cells. Analyses of stool samples of post-transplant PFIC1 patients demonstrated that bile salt content was not changed, whereas sodium and chloride concentrations were elevated and potassium levels were decreased. The ATP8B1-CDC50A heterodimer is essential for the apical localization of SLC10A2 in Caco-2 cells. Diarrhea in PFIC1/BRIC1 patients has a secretory origin to which SLC10A2 deficiency may contribute. This results in elevated luminal bile salt concentrations and consequent enhanced electrolyte secretion and/or reduced electrolyte resorption.

  11. Ric-8B interacts with G alpha olf and G gamma 13 and co-localizes with G alpha olf, G beta 1 and G gamma 13 in the cilia of olfactory sensory neurons.

    PubMed

    Kerr, Daniel S; Von Dannecker, Luiz Eduardo C; Davalos, Marcela; Michaloski, Jussara S; Malnic, Bettina

    2008-07-01

    Olfactory sensory neurons are able to detect odorants with high sensitivity and specificity. We have demonstrated that Ric-8B, a guanine nucleotide exchange factor (GEF), interacts with Galphaolf and enhances odorant receptor signaling. Here we show that Ric-8B also interacts with Ggamma13, a divergent member of the Ggamma subunit family which has been implicated in taste signal transduction, and is abundantly expressed in the cilia of olfactory sensory neurons. We show that Gbeta1 is the predominant Gbeta subunit expressed in the olfactory sensory neurons. Ric-8B and Gbeta1, like Galphaolf and Ggamma13, are enriched in the cilia of olfactory sensory neurons. We also show that Ric-8B interacts with Galphaolf in a nucleotide dependent manner, consistent with the role as a GEF. Our results constitute the first example of a GEF protein that interacts with two different olfactory G protein subunits and further implicate Ric-8B as a regulator of odorant signal transduction. PMID:18462949

  12. The primary structure of component 8c-1, a subunit protein of intermediate filaments in wool keratin. Relationships with proteins from other intermediate filaments.

    PubMed Central

    Dowling, L M; Crewther, W G; Inglis, A S

    1986-01-01

    Component 8c-1, one of four highly homologous component-8 subunit proteins present in the microfibrils of wool, was isolated as its S-carboxymethyl derivative and its amino acid sequence was determined. Large peptides were isolated after cleaving the protein chemically or enzymically and the sequence of each was determined with an automatic Sequenator. The peptides were ordered by sequence overlaps and, in some instances, by homology with known sequences from other component-8 subunits. The C-terminal residues were identified by three procedures. Full details of the various procedures used have been deposited as Supplementary Publication SUP 50133 (4 pp.) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1986) 233, 5. The result showed that the protein comprises 412 residues and has an Mr, including the N-terminal acetyl group, of 48,300. The sequence of residues 98-200 of component 8c-1 was found to correspond to the partial or complete sequences of four homologous type I helical segments previously isolated from helical fragments recovered from chymotryptic digests of microfibrillar proteins of wool [Crewther & Dowling (1971) Appl. Polym. Symp. 18, 1-20; Crewther, Gough, Inglis & McKern (1978) Text. Res. J. 48, 160-162; Gough, Inglis & Crewther (1978) Biochem. J. 173, 385]. Considered in relation to amino acid sequences of other intermediate-filament proteins, the sequence is in accord with the view that keratin filament proteins are of two types [Hanukoglu & Fuchs (1983) Cell (Cambridge, Mass.) 33, 915-924]. Filament proteins from non-keratinous tissues, such as desmin, vimentin, neurofilament proteins and the glial fibrillary acidic protein, which form monocomponent filaments, constitute a third type. It is suggested that as a whole the proteins from intermediate filaments be classed as filamentins, the three types at present identified forming

  13. Crystal structures of two novel borate compounds MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8}

    SciTech Connect

    Li, H.K.; Cai, G.M.; Fan, J.J.; Jin, Z.P.; Zhou, T.T.; Chen, X.L.

    2013-06-15

    Two novel borate compounds MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8}, have been synthesized via solid-state reactions, and their crystal structures have been solved and refined from powder X-ray diffraction data. The compound MgInBO{sub 4}, which was obtained at 1190 °C, belongs to the warwickite family. It crystallizes in the Pnma space group (no. 62) with a=9.5443(1) Å, b=3.2771(1) Å, c=9.5228(1) Å, and Z=4. The fundamental building units are liner –Mg(In)O{sub 6}–[In(Mg)]{sub 2}O{sub 10}–Mg(In)O{sub 6}– chains and isolated BO{sub 3} triangles. The low-temperature phase, MgIn{sub 7/8}B{sub 7/8}O{sub 29/8}, whose crystal structure is solved ab initio by the charge-flipping method with standard chemical formula MgInBO{sub 4}, was prepared at 1080 °C. It crystallizes in the P12{sub 1}/n1 space group (no. 14) with a=17.0976(1) Å, b=3.2504(1) Å, c=5.3387(1) Å, β=96.0829(3)°, and Z=4. The structure of MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} contains [In(2)/Mg(2)]{sub 2}O{sub 10} groups, –MgO{sub 6}–InO{sub 6}– infinite ribbons and isolated BO{sub 3} triangles. The experiments and the differential thermal analysis (DTA) show the decompositions of MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} happen at about 1220 °C and 1180 °C, respectively. The comparative crystal chemistry from MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} to MgInBO{sub 4} has been discussed. Infrared spectra and UV–vis diffuse reflectance spectra of MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} were measured. - Graphical abstract: The structural transformation from the monoclinic MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} (P12{sub 1}/n1) to the orthorhombic MgInBO{sub 4} (Pnma). - Highlights: • Two novel borate compounds MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} have been synthesized via solid-state reactions for the first time. • The crystal structures of MgInBO{sub 4} and MgIn{sub 7/8}B{sub 7/8}O{sub 29/8} have been solved and refined from powder X

  14. Ring Puckering Potentials of Three Fluorinated Cyclopentenes: C_5F_8, C_5HF_7, and C_5H_2F_6

    NASA Astrophysics Data System (ADS)

    Arsenault, E. A.; Long, B. E.; Pringle, Wallace C.; Choi, Yoon Jeong; Cooke, S. A.; Ocola, Esther J.; Laane, Jaan

    2015-06-01

    A systematic study on the ring puckering potentials of three fluorinated cyclopentenes has been performed using Fourier transform microwave spectroscopy in tandem with quantum chemical calculations. Spectra between 8 GHz and 16 GHz have been measured for octafluorocyclopentene, 1H-heptafluorocyclopentene, and 1H,2H-hexafluorocyclopentene, where the hydrogens sequentially replace the fluorines on the sp^2 hybridized carbons. Rotational constants and centrifugal distortion constants have been determined for the parent species and all 13C isotopologues. In regards to the ring puckering, double minimum potential, both cross state and intra-state transitions were observed for all molecules except the 1H,2H-hexafluorocyclopentene. Experimental Coriolis coupling constants and ΔE01 values will be presented and discussed. The ring puckering barrier heights for C_5F_8, C_5HF_7, and C_5H_2F_6, have been calculated to be 222 wn, 302 wn, and 367 wn, respectively.

  15. Expression pattern of NMDA receptors reveals antiepileptic potential of apigenin 8-C-glucoside and chlorogenic acid in pilocarpine induced epileptic mice.

    PubMed

    Aseervatham, G Smilin Bell; Suryakala, U; Doulethunisha; Sundaram, S; Bose, P Chandra; Sivasudha, T

    2016-08-01

    The present study was aimed to evaluate the effect of apigenin 8-C-glucoside (Vitexin) and chlorogenic acid on epileptic mice induced by pilocarpine and explored its possible mechanisms. Intraperitonial administration of pilocarpine (85mg/kg) induced seizure in mice was assessed by behavior observations, which is significantly (p>0.05) reduced by apigenin 8-C-glucoside (AP8CG) (10mg/kg) and chlorogenic acid (CA) (5mg/kg), similar to diazepam. Seizure was accompanied by an imbalance in the levels of Gamma-aminobutyric acid (GABA) and glutamate in the pilocarpine administered group. Moreover, convulsion along with reduced acetylcholinesterase, increased monoamine oxidase and oxidative stress was observed in epileptic mice brain. AP8CG and CA significantly restored back to normal levels even at lower doses. Further, increased lipid peroxidation and nitrite content was also significantly attenuated by AP8CG and CA. However, CA was found to be more effective when compared to AP8CG. In addition, the mRNA expression of N-methyl-d-aspartate receptor (NMDAR), mGluR1 and mGlu5 was significantly (P≤0.05) inhibited by AP8CG and CA in a lower dose. The mRNA expression of GRIK1 did not differ significantly in any of the group and showed a similar pattern of expression. Our result shows that AP8CG and CA selectively inhibit NMDAR, mGluR1 and mGlu5 expression. Modification in the provoked NMDAR calcium response coupled with neuronal death. Hence, these findings underline that the polyphenolics, AP8CG and CA have exerted antiepileptic and neuroprotective activity by suppressing glutamate receptors. PMID:27470339

  16. HWMA/RCRA CLOSURE PLAN FOR THE MATERIALS TEST REACTOR WING (TRA-604) LABORATORY COMPONENTS VOLUNTARY CONSENT ORDER ACTION PLAN VCO-5.8 D REVISION2

    SciTech Connect

    KIRK WINTERHOLLER

    2008-02-25

    This Hazardous Waste Management Act/Resource Conservation and Recovery Act closure plan was developed for the laboratory components of the Test Reactor Area Catch Tank System (TRA-630) that are located in the Materials Test Reactor Wing (TRA-604) at the Reactor Technology Complex, Idaho National Laboratory Site, to meet a further milestone established under Voluntary Consent Order Action Plan VCO-5.8.d. The TRA-604 laboratory components addressed in this closure plan were deferred from the TRA-630 Catch Tank System closure plan due to ongoing laboratory operations in the areas requiring closure actions. The TRA-604 laboratory components include the TRA-604 laboratory warm wastewater drain piping, undersink drains, subheaders, and the east TRA-604 laboratory drain header. Potentially contaminated surfaces located beneath the TRA-604 laboratory warm wastewater drain piping and beneath the island sinks located in Laboratories 126 and 128 (located in TRA-661) are also addressed in this closure plan. The TRA-604 laboratory components will be closed in accordance with the interim status requirements of the Hazardous Waste Management Act/Resource Conservation and Recovery Act as implemented by the Idaho Administrative Procedures Act 58.01.05.009 and 40 Code of Federal Regulations 265, Subparts G and J. This closure plan presents the closure performance standards and the methods for achieving those standards.

  17. Phase 2 program on ground test of refanned JT8D turbofan engines and nacelles for the 727 airplane. Volume 4: Airplane evaluation and analysis

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The retrofit of JT8D-109 (refan) engines are evaluated on a 727-200 airplane in terms of airworthiness, performance, and noise. Design of certifiable hardware, manufacture, and ground testing of the essential nacelle components is included along with analysis of the certifiable airplane design to ensure airworthiness compliance and to predict the in-flight performance and noise characteristics of the modified airplane. The analyses confirm that the 727 refan airplane is certifiable. The refan airplane range would be 15% less that of the baseline airplane and block fuel would be increased by 1.5% to 3%. However, with this particular 727-200 model, with a brake release gross weight of 172,500 lb (78,245 kg), it is possible to operate the airplane (with minor structural modifications) at higher gross weights and increase the range up to 15% over the 727-200 (baseline) airplane. The refan airplane FAR Part 36 noise levels would be 6 to 8 EPNdB (effective perceived noise in decibels) below the baseline. Noise footprint studies showed that approach noise contour areas are small compared to takeoff areas. The 727 refan realizes a 68% to 83% reduction in annoyance-weighted area when compared to the 727-200 over a range of gross weights and operational procedures.

  18. Flight effects on JT8D engine jet noise as measured in the NASA Ames 40-by 80-foot wind tunnel

    NASA Technical Reports Server (NTRS)

    Strout, F. G.; Atencio, A., Jr.

    1976-01-01

    A JT8D-17 turbofan engine was tested in a 40 x 80 ft wind tunnel to determine flight effects on jet noise. The engine was configured as a baseline with conical nozzle, a quiet nacelle 20-lobe ejector/suppressor, and an internal mixer with conical nozzle. Tunnel-off and tunnel-on noise tests were conducted over a range of nozzle pressure ratios (1.2 to 2.1), primary jet velocities (275 to 550 m/s), and tunnel velocities up to 100 m/s. Aft quadrant noise data were measured by a pair of traversing microphones located on a 3-m sideline relative to the engine centerline. Unique correlations and analysis procedures were developed in order to define far-field flight effects from the relatively near-field noise measurements. The ejector/suppressor experienced a significant loss of suppression relative to static measurements during flight while the internal mixer indicated a slight gain in suppression. It is concluded that the wind tunnel is a viable method for studying flight effects on engine jet noise.

  19. Influence of long (16L:8D) and short (8L:16D) photoperiods on blood metabolites and hepatic metabolism in Olive flounder, Paralichthys olivaceus.

    PubMed

    Zou, Huafeng; Bai, Xianshou; Feng, Yuhong; Zhang, Ying; Wang, Youji; Lu, Weiqun

    2016-01-01

    In the present study the influence of long photoperiod (LP, 16L:8D) and short photoperiod (SP, 8L:16D) on hepatic energy metabolism in the olive flounder (Paralichthys olivaceus) was investigated. Flounders were maintained under LP or SP conditions for 2 weeks then plasmatic and hepatic parameters were assessed. At the plasmatic level, the concentration of cortisol was enhanced in flounder maintained under LP compared to SP. Alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzyme activities in plasma also increased in LP flounder. There was no significant difference in plasma glucose levels between the two experimental groups. Plasma osmotic pressure, Na and Cl levels were significantly higher in LP compared to the SP group. In liver, a significant decrease of triglycerides together with an increase in glycogen was observed in the LP group. Hepatic hsl and pepck and muscle hsl mRNA expression in LP was significantly higher in the SP group. Overall the results indicate that the LP treatment caused a mild stress response and increased hepatic energy metabolism in the flounder, which in turn could affect osmoregulation. In conclusion, it would appear that LP treatment can adversely influence hepatic energy metabolism in adult olive flounder under fasting condition. PMID:27386368

  20. Luteolin 8-C-β-fucopyranoside inhibits invasion and suppresses TPA-induced MMP-9 and IL-8 via ERK/AP-1 and ERK/NF-κB signaling in MCF-7 breast cancer cells.

    PubMed

    Park, Su-Ho; Kim, Jung-Hee; Lee, Dong-Hun; Kang, Jeong-Woo; Song, Hyuk-Hwan; Oh, Sei-Ryang; Yoon, Do-Young

    2013-11-01

    Matrix metalloproteinase 9 (MMP-9) and interleukin-8 (IL-8) play major roles in tumor progression and invasion of breast cancer cells. The present study was undertaken to investigate the inhibitory mechanism of cell invasion by luteolin 8-C-β-fucopyranoside (named as LU8C-FP), a C-glycosylflavone, in human breast cancer cells. We investigated whether LU8C-FP would inhibit MMP-9 activation and IL-8 expression in 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MCF-7 breast cancer cells. LU8C-FP suppressed TPA-induced MMP-9 and IL-8 secretion and mRNA expression via inhibition of the MAPK signaling pathway and down-regulation of nuclear AP-1 and NF-κB. TPA-induced phosphorylation of ERK 1/2 was suppressed by LU8C-FP, whereas JNK and p38 MAPK phosphorylation were unaffected. In addition, LU8C-FP blocked the ERK 1/2 pathways following expression of MMP-9 and IL-8. These results suggest LU8C-FP may function to suppress invasion of breast cancer cells through the ERK/AP-1 and ERK/NF-κB signaling cascades.

  1. DC-9/JT8D refan, Phase 1. [technical and economic feasibility of retrofitting DC-9 aircraft with refan engine to achieve desired acoustic levels

    NASA Technical Reports Server (NTRS)

    1973-01-01

    Analyses and design studies were conducted on the technical and economic feasibility of installing the JT8D-109 refan engine on the DC-9 aircraft. Design criteria included minimum change to the airframe to achieve desired acoustic levels. Several acoustic configurations were studied with two selected for detailed investigations. The minimum selected acoustic treatment configuration results in an estimated aircraft weight increase of 608 kg (1,342 lb) and the maximum selected acoustic treatment configuration results in an estimated aircraft weight increase of 809 kg (1,784 lb). The range loss for the minimum and maximum selected acoustic treatment configurations based on long range cruise at 10 668 m (35,000 ft) altitude with a typical payload of 6 804 kg (15,000 lb) amounts to 54 km (86 n. mi.) respectively. Estimated reduction in EPNL's for minimum selected treatment show 8 EPNdB at approach, 12 EPNdB for takeoff with power cutback, 15 EPNdB for takeoff without power cutback and 12 EPNdB for sideline using FAR Part 36. Little difference was estimated in EPNL between minimum and maximum treatments due to reduced performance of maximum treatment. No major technical problems were encountered in the study. The refan concept for the DC-9 appears technically feasible and economically viable at approximately $1,000,000 per airplane. An additional study of the installation of JT3D-9 refan engine on the DC-8-50/61 and DC-8-62/63 aircraft is included. Three levels of acoustic treatment were suggested for DC-8-50/61 and two levels for DC-8-62/63. Results indicate the DC-8 technically can be retrofitted with refan engines for approximately $2,500,000 per airplane.

  2. Biological half-lives and organ distribution of tritiated 8-lysine-vasopressin and 1-deamino-8-D-arginine-vasopressin in Brattleboro rats

    SciTech Connect

    Janaky, T.; Laczi, F.; Laszlo, F.A.

    1982-01-01

    The biological half-lives and organ distribution of tritiated 8-lysine-vasopressin and 1-deamino-8-D-arginine-vasopressin were determined in R-Amsterdam rats and in homozygous and heterozygous Brattleboro rats with hereditary central diabetes insipidus. It was found that the biological half-lives of (/sup 3/H)LVP and (/sup 3/H)dDAVP in the Brattleboro rats did not differ significantly from that found in the control R-Amsterdam rats. The half-life of (/sup 3/H)dDAVP proved longer than that of (/sup 3/H)LVP in all three groups of animals. In the case of (/sup 3/H)LVP the highest radioactivities were observed in the neurohypophyses, adenohypophyses, and kidneys of both the R-Amsterdam and Brattleboro rats. The accumulation of tritiated material was higher in the small intestine of the Brattleboro rats than in that of the R-Amsterdam animals. In all three groups of rats, (/sup 3/H)dDAVP was accumulated to the greatest extent in the kidney and the small intestine. The kidney and small intestine contained less radioactivity in homozygous Brattleboro rats than in the controls. There was only a slight radioactivity accumulation in the adenohypophysis and neurohypophysis. From the results it was concluded that the decrease in the rate of enzymatic decomposition may play a role in the increased duration of antidiuretic action of dDAVP. The results have led to the conclusion that the accelerated elimination of vasopressin and its pathologic organ accumulation are probably not involved in the water metabolism disturbance of Brattleboro rats with hereditary diabetes insipidus.

  3. "Dancing inside the ball": the structures and nonlinear optical properties of three Sc2S@C3v(8)-C82 isomers.

    PubMed

    Gao, Feng-Wei; Gao, Ying; Wang, Li-Jie; Xu, Hong-Liang; Sun, Shi-Ling; Su, Zhong-Min

    2015-10-01

    Recently, the crystal structures and electrochemical properties of the isomers (Sc2S "trapped" in C82) have been reported, in which the Sc2S is located inside the different positions of the C82 cage. In the present work, three isomers of endohedral metallofullerenes Sc2S@C3v(8)-C82 (A, B, and C) have been designed to explore the effect of the position of Sc2S on their interaction energies and nonlinear optical properties. Among three isomers, the Sc2S is located in different positions of the C82 cage: the angles of Sc-S-Sc in A, B, and C are 104.9, 114.8, and 115.7°, respectively. Furthermore, the analysis of natural bond orbital (NBO) charge indicates that the electron-transfer is from the Sc2S to the adjacent carbon atoms of the C82 cage. The interaction energy of B is the smallest among three isomers which is -226.2 kcal mol(-1). It was worth mentioning that their first hyperpolarizabilities (β tot) were studied, we found that their β tot values were related to the positions of Sc2S: C (2100) > B (1191) > A (947 au). We hope that the present work can provide a new strategy to promote the nonlinear optical properties of endohedral metallofullerenes by changing the positions of the encapsulated molecular. Graphical abstract Three isomers of endohedral metallofullerenes Sc2S@C3v(8)-C82 (A, B, and C) have been designed to explore the position effect of Sc2S on the interaction energies and nonlinear optical properties. Among three isomers, the Sc2S in B has the most stable position. Significantly, the first hyperpolarizability is related to the position of Sc2S inside the C82 cage, which provides a novel strategy to enhance the first hyperpolarizability by the Sc2S revolving inside the C82 cage.

  4. Nearly Perfect Spin Filter Based on a Wire of Half-Metallic (η5-C5H5)Ti (η8-C8H8)Ti Units

    NASA Astrophysics Data System (ADS)

    Zhu, Sicong; Fang, Jianming; Yao, Kailun; Wu, Yanqing

    2015-07-01

    Organic molecular devices show strong potential for future nanoelectronics applications, many of which require multifunction capability. Organometallic [(C5H5)Ti(C8H8)] n clusters are one of the few molecular material clusters that have been synthesized experimentally. Here we present a theoretical study of the spin-polarized transport on the clusters of [(C5H5)Ti(C8H8)] n by using nonequilibrium Green's functions combined with the density-functional theory. Results indicate that one-dimensional, organic (η5-C5H5)Ti (η8-C8H8)Ti wire has unique half-metallic properties with 100% spin polarization near the Fermi level in the ground state. We predict that finite-layer [(C5H5)Ti (C8H8)] n clusters coupled to gold electrodes exhibit a near-perfect spin-filter effect. Furthermore, due to the large spin coherence originated from its organic nature, these devices possess a spin-valve effect with large magnetoresistance. Our findings suggest that such a multifunctional molecular device is an excellent candidate for future molecule-based spintronic applications.

  5. 40 CFR Appendix A to Subpart Bb of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... performance specifications. 63.8(a)(3) No . 63.8(a)(4) Yes. 63.8(b) Conduct of Monitoring Yes. 63.8(c)(1... or CMS performance specifications. 63.8(d) Quality Control Yes. 63.8(e) CMS Performance Evaluation No...)(6) No Subpart BB does not require CMS performance specifications. 63.10(c)(7) and (8) Yes....

  6. Chimeric Antibody c.8B6 to O-Acetyl-GD2 Mediates the Same Efficient Anti-Neuroblastoma Effects as Therapeutic ch14.18 Antibody to GD2 without Antibody Induced Allodynia

    PubMed Central

    Cochonneau, Denis; Chaumette, Tanguy; Xiao, Wenhua; Diccianni, Mitchell B.; Barbet, Jacques; Yu, Alice L.; Paris, François; Sorkin, Linda S.; Birklé, Stéphane

    2014-01-01

    Background Anti-GD2 antibody is a proven therapy for GD2-postive neuroblastoma. Monoclonal antibodies against GD2, such as chimeric mAb ch14.18, have become benchmarks for neuroblastoma therapies. Pain, however, can limit immunotherapy with anti-GD2 therapeutic antibodies like ch14.18. This adverse effect is attributed to acute inflammation via complement activation on GD2-expressing nerves. Thus, new strategies are needed for the development of treatment intensification strategies to improve the outcome of these patients. Methodology/Principal Findings We established the mouse-human chimeric antibody c.8B6 specific to OAcGD2 in order to reduce potential immunogenicity in patients and to fill the need for a selective agent that can kill neuroblastoma cells without inducing adverse neurological side effects caused by anti-GD2 antibody immunotherapy. We further analyzed some of its functional properties compared with anti-GD2 ch14.18 therapeutic antibody. With the exception of allodynic activity, we found that antibody c.8B6 shares the same anti-neuroblastoma attributes as therapeutic ch14.18 anti-GD2 mAb when tested in cell-based assay and in vivo in an animal model. Conclusion/Significance The absence of OAcGD2 expression on nerve fibers and the lack of allodynic properties of c.8B6–which are believed to play a major role in mediating anti-GD2 mAb dose-limiting side effects–provide an important rationale for the clinical application of c.8B6 in patients with high-risk neuroblastoma. PMID:24520328

  7. In-depth understanding of the chemical properties of rarely explored carbide cluster metallofullerenes: a case study of Sc2 C2 @C3v (8)-C82 that reveals a general rule.

    PubMed

    Cai, Wenting; Chen, Muqing; Bao, Lipiao; Xie, Yunpeng; Akasaka, Takeshi; Lu, Xing

    2015-02-16

    The chemical properties of carbide-cluster metallofullerenes (CCMFs) remain largely unexplored, although several new members of CCMFs have been discovered recently. Herein, we report the reaction between Sc2 C2 @C3v (8)-C82 , which is viewed as a prototypical CCMF because of its high abundance, and 3-triphenylmethyl-5-oxazolidinone (1) to afford the corresponding pyrrolidino derivative Sc2 C2 @C3v (8)-C82 (CH2 )2 NTrt (2; Trt=triphenylmethyl). Single-crystal X-ray crystallography studies of 2 revealed that the reaction takes place at a [6,6]-bond junction, which is directly over the encapsulated C2 unit and is far from either of the two scandium atoms. On the basis of theoretical calculations and by considering previously reports, we have found that a hexagonal carbon ring on the cage of Sc2 C2 @C3v (8)-C82 is highly reactive toward different reagents due to the overlap of high p-orbital axis vector (POAV) angles and large LUMO coefficients. We propose that this highly concentrated area of reactivity is generated by the encapsulation of the Sc2 C2 cluster because this region is absent from the empty fullerene C3v (8)-C82 . Moreover, the absorption and electrochemical results confirm that derivative 2 is more stable than pristine Sc2 C2 @C3v (8)-C82 , thus illuminating its potential applications.

  8. ATOMIC AND MOLECULAR PHYSICS: Interference Angle on Quantum Rotational Energy Transfer in Na + Na2 (A1 Σ+u, v = 8 ~ b3п0u, v = 14) Molecular Collision System

    NASA Astrophysics Data System (ADS)

    Wang, Wei-Li; Miao, Gang; Li, Jian; Ma, Feng-Cai

    2009-12-01

    In order to study the collisional quantum interference (CQI) on rotational energy transfer in atom-diatom system, we have studied the relation of the integral interference angle and differential interference angle in Na + Na2 (A1 σ+u, v = 8 ~ b3п0u, v = 14) collision system. In this paper, based on the first-Born approximation of time-dependent perturbation theory and taking into accounts the anisotropic effect of Lennard-Jones interaction potentials, we present a theoretical model of collisional quantum interference in intramolecular rotational energy transfer, and a relationship between differential and integral interference angles.

  9. Flight effects on noise generated by the JT8D-17 engine in a quiet nacelle and a conventional nacelle as measured in the NASA-Ames 40- by 80-foot wind tunnel

    NASA Technical Reports Server (NTRS)

    Strout, F. G.

    1976-01-01

    A JT8D-17 turbofan engine was tested in the NASA-Ames 40- by 80-foot wind tunnel to determine flight effects on jet and fan noise. Baseline, quiet nacelle with 20-lobe ejector/suppressor, and internal mixer configurations were tested over a range of engine power settings and tunnel velocities. Flight effects derived from the 40- by 80-foot wind tunnel test are compared with 727/JT8D flight test data and with model data obtained in a smaller wind tunnel. Procedures are defined for measuring noise data in a wind tunnel relatively near the sources and analyzing the results to obtain far-field flight effects. Wind tunnel and 727 flight test noise results compare favorably for both the baseline and quiet nacelle configurations. Two reports are provided, including a comprehensive version with extensive test results and analysis and the subject summary version that emphasizes data analysis and program finding.

  10. Association of the Variants CASP8 D302H and CASP10 V410I with Breast and Ovarian Cancer Risk in BRCA1 and BRCA2 Mutation Carriers

    PubMed Central

    Engel, Christoph; Versmold, Beatrix; Wappenschmidt, Barbara; Simard, Jacques; Easton, Douglas F.; Peock, Susan; Cook, Margaret; Oliver, Clare; Frost, Debra; Mayes, Rebecca; Evans, D. Gareth; Eeles, Rosalind; Paterson, Joan; Brewer, Carole; McGuffog, Lesley; Antoniou, Antonis C.; Stoppa-Lyonnet, Dominique; Sinilnikova, Olga M.; Barjhoux, Laure; Frenay, Marc; Michel, Cécile; Leroux, Dominique; Dreyfus, Helene; Toulas, Christine; Gladieff, Laurence; Uhrhammer, Nancy; Bignon, Yves-Jean; Meindl, Alfons; Arnold, Norbert; Varon-Mateeva, Raymonda; Niederacher, Dieter; Preisler-Adams, Sabine; Kast, Karin; Deissler, Helmut; Sutter, Christian; Gadzicki, Dorothea; Chenevix-Trench, Georgia; Spurdle, Amanda B.; Chen, Xiaoqing; Beesley, Jonathan; Olsson, Håkan; Kristoffersson, Ulf; Ehrencrona, Hans; Liljegren, Annelie; van der Luijt, Rob B.; van Os, Theo A.; van Leeuwen, Flora E.; Domchek, Susan M.; Rebbeck, Timothy R.; Nathanson, Katherine L.; Osorio, Ana; Cajal, Teresa Ramón y; Konstantopoulou, Irene; Benítez, Javier; Friedman, Eitan; Kaufman, Bella; Laitman, Yael; Mai, Phuong L.; Greene, Mark H.; Nevanlinna, Heli; Aittomäki, Kristiina; Szabo, Csilla I.; Caldes, Trinidad; Couch, Fergus J.; Andrulis, Irene L.; Godwin, Andrew K.; Hamann, Ute; Schmutzler, Rita K.

    2011-01-01

    Background The genes caspase-8 (CASP8) and caspase-10 (CASP10) functionally cooperate and play a key role in the initiation of apoptosis. Suppression of apoptosis is one of the major mechanisms underlying the origin and progression of cancer. Previous case-control studies have indicated that the polymorphisms CASP8 D302H and CASP10 V410I are associated with a reduced risk of breast cancer in the general population. Methods To evaluate whether the CASP8 D302H (CASP10 V410I) polymorphisms modify breast or ovarian cancer risk in BRCA1 and BRCA2 mutation carriers, we analyzed 7,353 (7,227) subjects of white European origin provided by 19 (18) study groups that participate in the Consortium of Investigators of Modifiers of BRCA1/2 (CIM-BA). A weighted cohort approach was used to estimate hazard ratios (HR) and 95% confidence intervals (95% CI). Results The minor allele of CASP8 D302H was significantly associated with a reduced risk of breast cancer (per-allele HR, 0.85; 95% CI, 0.76–0.97; Ptrend = 0.011) and ovarian cancer (per-allele HR, 0.69; 95% CI, 0.53–0.89; Ptrend = 0.004) for BRCA1 but not for BRCA2 mutation carriers. The CASP10 V410I polymorphism was not associated with breast or ovarian cancer risk for BRCA1 or BRCA2 mutation carriers. Conclusions CASP8 D302H decreases breast and ovarian cancer risk for BRCA1 mutation carriers but not for BRCA2 mutation carriers. Impact The combined application of these and other recently identified genetic risk modifiers could in the future allow better individual risk calculation and could aid in the individualized counseling and decision making with respect to preventive options in BRCA1 mutation carriers. PMID:20978178

  11. The oxygen content of the high-temperature superconducting compound Bi(2+x)Sr(3-y)CayCu2O(8+d) with respect to varying Ca and Bi contents

    NASA Technical Reports Server (NTRS)

    Majewski, P.; Su, H.-L.; Aldinger, F.

    1995-01-01

    The oxygen content of Bi(2+x)Sr(3-y)Cu2O(8+d) (2212 phase) has been determined as a function of its cation concentration. With increasing Ca and Bi content the oxygen content increases and T(sub c) decreases. The oxygen content of Ca rich 2212 phase increases with decreasing annealing temperatures. The study shows that the T(sub c) of the 2212 phase primarily is controlled by its cation concentration.

  12. Flight effects on noise by the JT8D engine with inverted primary/fan flow as measured in the NASA-Ames 40 by 80 foot wind tunnel

    NASA Technical Reports Server (NTRS)

    Strout, F. G.

    1978-01-01

    A JT8D-17R engine with inverted primary and fan flows was tested under static conditions as well as in the NASA Ames 40 by 80 Foot Wind Tunnel to determine static and flight noise characteristics, and flow profile of a large scale engine. Test and analysis techniques developed by a previous model and JT8D engine test program were used to determine the in-flight noise. The engine with inverted flow was tested with a conical nozzle and with a plug nozzle, 20 lobe nozzle, and an acoustic shield. Wind tunnel results show that forward velocity causes significant reduction in peak PNL suppression relative to uninverted flow. The loss of EPNL suppression is relatively modest. The in-flight peak PNL suppression of the inverter with conical nozzle was 2.5 PNdb relative to a static value of 5.5 PNdb. The corresponding EPNL suppression was 4.0 EPNdb for flight and 5.0 EPNdb for static operation. The highest in-flight EPNL suppression was 7.5 EPNdb obtained by the inverter with 20 lobe nozzle and acoustic shield. When compared with the JT8D engine with internal mixer, the inverted flow configuration provides more EPNL suppression under both static and flight conditions.

  13. Crystal structure and Mössbauer studies of the isotypic Fe{sub 6}-cluster compounds RE{sub 15}[Fe{sub 8}C{sub 25}], RE=Dy, Ho

    SciTech Connect

    Davaasuren, Bambar; Dashjav, Enkhtsetseg; Kreiner, Guido; Borrmann, Horst; Weber, Sven-Ulf; Becker, Klaus-Dieter; Jochen Litterst, Fred; Kniep, Rüdiger

    2015-05-15

    The carboferrates RE{sub 15}[Fe{sub 8}C{sub 25}] (RE=Dy, Ho) were prepared from mixtures of the elements by arc-melting followed with subsequent annealing at 1373 K. The crystal structures were determined from single crystal X-ray diffraction data and revealed an isotypic relationship to Er{sub 15}[Fe{sub 8}C{sub 25}] (hP48, P321). The main feature of the crystal structure is given by Fe{sub 6} cluster units characterized by covalent Fe–Fe bonding interactions. {sup 57}Fe Mössbauer spectra of Dy{sub 15}[Fe{sub 8}C{sub 25}] were fitted by three subspectra with relative spectral weights of about 3:3:2 which is in general agreement with the crystal structure. Below 50 K, an onset of magnetic hyperfine fields at the three iron sites is observed which is supposed to be caused by dipolar fields arising from neighboring, slowly relaxing Dy magnetic moments. - Graphical abstract: Fe{sub 6}-cluster in the crystal structure of RE{sub 15}[Fe{sub 8}C{sub 25}], RE=Dy, Ho. - Highlights: • New carboferrates RE{sub 15}[Fe{sub 8}C{sub 25}] with RE=Dy, Ho have been synthesized. • The crystal structures were refined using single crystal X-ray data. • An orientational relationship between Fe{sub 6}-clusters and Fe in γ-Fe is outlined. • {sup 57}Fe Mössbauer spectra are in agreement with structural data from X-rays. • Magnetic hyperfine fields below 50 K are explained by dipolar fields from Dy atoms.

  14. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... syndrome, emotional or mental illness, and specific learning disabilities; (C) The term “physical or mental... disease, diabetes, mental retardation, emotional illness, drug addiction and alcoholism. (ii) Major life...) Handicapped person—(1) Handicapped person means any person who: (i) Has a physical or mental impairment...

  15. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... syndrome, emotional or mental illness, and specific learning disabilities; (C) The term “physical or mental... disease, diabetes, mental retardation, emotional illness, drug addiction and alcoholism. (ii) Major life...) Handicapped person—(1) Handicapped person means any person who: (i) Has a physical or mental impairment...

  16. 15 CFR 8b.8 - Notice.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... participants, beneficiaries, applicants and employees, including those with impaired vision or hearing, and... available to persons with impaired vision or hearing. (b) If a recipient publishes or uses...

  17. 15 CFR 8b.25 - Nonacademic services.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... athletics shall provide to qualified handicapped students an equal opportunity for participation in these... of particular careers. (c) Social organizations. A recipient that provides significant assistance...

  18. 15 CFR 8b.17 - Existing facilities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... buildings, assignment of aides to beneficiaries, home visits, delivery of services at alternate accessible... accessible or the aid, benefit, or service must be made available at an alternative accessible site or sites... alternative, refer the qualified handicapped individual to other providers of those services that...

  19. 15 CFR 8b.17 - Existing facilities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... buildings, assignment of aides to beneficiaries, home visits, delivery of services at alternate accessible... accessible or the aid, benefit, or service must be made available at an alternative accessible site or sites... alternative, refer the qualified handicapped individual to other providers of those services that...

  20. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... more of the following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genito-urinary; hemic and lymphatic; skin... postsecondary institution, or a public system of higher education; or (ii) A local educational agency...

  1. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... more of the following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genito-urinary; hemic and lymphatic; skin... postsecondary institution, or a public system of higher education; or (ii) A local educational agency...

  2. 15 CFR 8b.3 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... interest in such property. (e) Federal financial assistance means any grant, loan, contract (other than a... Federal personnel; or (3) Real and personal property or any interest in or use of such property, including... syndrome, emotional or mental illness, and specific learning disabilities; (C) The term “physical or...

  3. 15 CFR 8b.13 - Employment criteria.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... sensory, manual, or speaking skills, the test results accurately to reflect the applicant's or employee's... applicant's or employee's impaired sensory, manual, or speaking skills (except where those skills are...

  4. 15 CFR 8b.12 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... physical or metal limitations of an otherwise qualified handicapped applicant or employee unless the...'s operation, including the composition and structure of the recipient's workforce; and (3)...

  5. 15 CFR 8b.14 - Preemployment inquiries.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., That: (1) The recipient states clearly on any written questionnaire used for this purpose or makes clear orally, if no written questionnaire is used, that the information requested is intended for...

  6. 15 CFR 8b.22 - Academic adjustments.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... that impairs sensory, manual, or speaking skills as will best ensure that the results of the evaluation..., manual, or speaking skills (except where such skills are the factors that the test purports to measure..., or speaking skills. A recipient shall operate each program or activity to which this subpart...

  7. High hard magnetic properties and cellular structure of nanocomposite magnet Nd 4.5Fe 73.8B 18.5Cr 0.5Co 1.5Nb 1Cu 0.2

    NASA Astrophysics Data System (ADS)

    The, N. D.; Chau, N.; Vuong, N. V.; Quyen, N. H.

    2006-08-01

    The formation of special nanostructure, cellular structure, in Nd 4.5Fe 73.8B 18.5Cr 0.5Co 1.5Nb 1Cu 0.2 nanocomposite magnet has been observed by means of SEM for the first time. Ultrafine structure of cellules with thickness of 20-25 nm and length in range of 200-300 nm leads to high shape anisotropy of the materials. Therefore, high hard magnetic properties were obtained with ( BH) max up to 17.3 MG Oe in ribbons with very high remanence of 13.5 kG. The role of Cr and Co in the formation and refinement of cellular structure is proposed. Effect of heat treatment on hard magnetic properties is discussed in detail.

  8. Biological assessment: possible impacts of exploratory drilling in sections 8B and 18H, Naval Petroleum Reserve No. 2, Kern County, California on the endangered San Joaquin kit fox, blunt-nosed leopard lizard, and other sensitive species

    SciTech Connect

    O'Farrell, T.P.; Sauls, M.L.

    1982-07-01

    The U.S. Department of Energy proposes to drill exploratory wells on two sections, 8B and 18H, within Naval Petroleum Reserve No. 2 in western Kern County, California. The proposed sites are thought to provide habitat for the endangered San Joaquin kit fox and blunt-nosed leopard lizard, as well as two sensitive species: the giant kangaroo rat and San Joaquin antelope ground squirrel. The objective was to assess the possible impacts of the exploratory drilling on these species and their essential habitats. Although 23 potential San Joaquin kit fox den sites were found during surveys of a total of 512 ha (1280 acres) surrounding both well sites, no burrows were closer than 30 m from proposed disturbance, and most were over 200 m away. Two blunt-nosed leopard lizards were observed on private land within 8B, one was observed on private land in 18H, and two were seen on DOE portions of 18H. No evidence of blunt-nosed leopard lizards was gathered in the immediate vicinity of either proposed well site. Although 5 ha of habitat will be disturbed, there is no evidence to indicate any of the species has burrows on-site that will be lost during land clearing. Loss of habitat will be mitigated during the cleanup and restoration phases when disturbed areas will be revegetated. Increased traffic, human activities, noise and ground vibration levels, as well as illumination throughout the night, may disturb the fauna. However, these species have adapted to intensive human disturbances on Elk Hills without obvious negative effects. The short duration of the project should allow any displaced animals to return to the sites after drilling ceases.

  9. Role of IncP-1β plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses.

    PubMed

    Król, J E; Penrod, J T; McCaslin, H; Rogers, L M; Yano, H; Stancik, A D; Dejonghe, W; Brown, C J; Parales, R E; Wuertz, S; Top, E M

    2012-02-01

    Broad-host-range catabolic plasmids play an important role in bacterial degradation of man-made compounds. To gain insight into the role of these plasmids in chloroaniline degradation, we determined the first complete nucleotide sequences of an IncP-1 chloroaniline degradation plasmid, pWDL7::rfp and its close relative pNB8c, as well as the expression pattern, function, and bioaugmentation potential of the putative 3-chloroaniline (3-CA) oxidation genes. Based on phylogenetic analysis of backbone proteins, both plasmids are members of a distinct clade within the IncP-1β subgroup. The plasmids are almost identical, but whereas pWDL7::rfp carries a duplicate inverted catabolic transposon, Tn6063, containing a putative 3-CA oxidation gene cluster, dcaQTA1A2BR, pNB8c contains only a single copy of the transposon. No genes for an aromatic ring cleavage pathway were detected on either plasmid, suggesting that only the upper 3-CA degradation pathway was present. The dcaA1A2B gene products expressed from a high-copy-number vector were shown to convert 3-CA to 4-chlorocatechol in Escherichia coli. Slight differences in the dca promoter region between the plasmids and lack of induction of transcription of the pNB8c dca genes by 3-CA may explain previous findings that pNB8C does not confer 3-CA transformation. Bioaugmentation of activated sludge with pWDL7::rfp accelerated removal of 3-CA, but only in the presence of an additional carbon source. Successful bioaugmentation requires complementation of the upper pathway genes with chlorocatechol cleavage genes in indigenous bacteria. The genome sequences of these plasmids thus help explain the molecular basis of their catabolic activities.

  10. The oxygen content of the high-temperature superconducting compound Bi{sub 2+x}Sr{sub 3-y}CayCu{sub 2}O{sub 8+d} with respect to varying Ca and Bi contents

    SciTech Connect

    Majewski, P.; Su, H.L.; Aldinger, F.

    1994-12-31

    The oxygen content of Bi{sub 2+x}Sr{sub 3-y}Ca{sub y}Cu{sub 2}O{sub 8+d} (2212 phase) has been determined as a function of its cation concentration. With increasing Ca and Bi content the oxygen content increases and T{sub c} decreases. The oxygen content of Ca rich 2212 phase increases with decreasing annealing temperatures. The study shows that the T{sub c} of the 2212 phase primarily is controlled by its cation concentration.

  11. Formation of metallaboranes from the group IV transition metals and pentaborane(9): crystal and molecular structure of [(Cp(2)Zr)(2)B(5)H(8)][B(11)H(14)].

    PubMed

    Thomas, Rhodri Ll; Rath, Nigam P; Barton, Lawrence

    2002-01-14

    The reactions between [(C(5)H(5))(2)MCl(2)] (where M = Ti, Zr, Hf) and Li[B(5)H(8)] in a variety of solvents have been investigated. In the case of Zr, a pale orange solid, mu-(Cp(2)ClZr)B(5)H(8) (1), is formed in 70% yield. Compound 1 exists as a B(5)H(9) cage with a Cp(2)ClZr moiety replacing a bridging H atom. The variable temperature NMR spectra of 1 reveal two fluxional processes, one (DeltaG(++) = 54 kJ mol(-1)) which renders a plane of symmetry in the molecule and a higher temperature one (DeltaG(++) = 48 kJ mol(-1)) which renders all the basal B atoms equivalent. Dynamic processes are suggested to account for these observations. Passage of a CH(2)Cl(2) solution of 1 through silica gel affords 2, [(Cp(2)Zr)(2)B(5)H(8)][B(11)H(14)], a yellow, air-stable, crystalline solid, in 14% yield. The cation in 2, [(Cp(2)Zr)(2)B(5)H(8)](+), consists of a distorted spiro[2.2]pentane-like B(5) moiety comprising two B(3) triangles sharing a naked boron vertex. The two triangles are twisted 73 degrees with respect to each other, and the two [Cp(2)Zr] groups bond in a trihapto arrangement to the two opposite B-B-B edges. Each exterior B-Zr edge is H-bridged, and the B atoms possess terminal hydrogens. Reactions of Cp(2)HfCl(2) with Li[B(5)H(8)] lead to the formation of the analogue of 2, [(Cp(2)Hf)(2)B(5)H(8)][B(11)H(14)] (3). The precursor to 3, that is, the Hf analogue of 1, is not observed. Reaction between Li[B(5)H(8)] and Cp(2)TiCl(2) afforded no identifiable products, but reaction with CpTiCl(3) resulted in cage coupling and the formation of B(10)H(14). PMID:11782145

  12. The results of a high-speed wind tunnel test to investigate the effects of the NASA refan JT8D engine nacelles on the stability and control characteristics of the Boeing 727 airplane

    NASA Technical Reports Server (NTRS)

    Kupcis, E. A.

    1973-01-01

    A high speed wind tunnel test was conducted to investigate the effects of the NASA Refan JT8D engine nacelles on the stability and control characteristics of the Boeing 727 airplane. The test was performed at the Calspan Corporation 8x8 ft. (2.44x2.44 m.) transonic wind tunnel. Both the 727-200 and -100 models were tested. A small nose-down pitching moment increment and a slight increase in longitudinal stability were noted due to the Refan nacelles. The directional stability of the 727-200 airplane increased up to 10 percent. A smaller improvement was observed on the 727-100 model. In general, the high speed stability and control characteristics of the basic airplane are not significantly altered by the Refan nacelle installation.

  13. The results of a low-speed wind tunnel test to investigate the effects of the Refan JT8D engine target thrust reverser on the stability and control characteristics of the Boeing 727-200 airplane

    NASA Technical Reports Server (NTRS)

    Kupcis, E. A.

    1974-01-01

    The effects of the Refan JT8D side engine target thrust reverser on the stability and control characteristics of the Boeing 727-200 airplane were investigated using the Boeing-Vertol 20 x 20 ft Low-Speed Wind Tunnel. A powered model of the 727-200 was tested in groud effect in the landing configuration. The Refan target reverser configuration was evaluated relative to the basic production 727 airplane with its clamshell-deflector door thrust reverser design. The Refan configuration had slightly improved directional control characteristics relative to the basic airplane. Clocking the Refan thrust reversers 20 degrees outboard to direct the reverser flow away from the vertical tail, had little effect on directional control. However, clocking them 20 degrees inboard resulted in a complete loss of rudder effectiveness for speeds greater than 90 knots. Variations in Refan reverser lip/fence geometry had a minor effect on directional control.

  14. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genitourinary; hemic and lymphatic; skin; and endocrine... that term is defined for purposes of employment in 29 CFR 1613.702(f), which is made applicable to...

  15. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genitourinary; hemic and lymphatic; skin; and endocrine... that term is defined for purposes of employment in 29 CFR 1613.702(f), which is made applicable to...

  16. 15 CFR 8c.40 - Employment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... discrimination in employment under any program or activity conducted by the agency. The definitions, requirements, and procedures of section 501 of the Rehabilitation Act of 1973 (29 U.S.C. 791), as established by the Equal Employment Opportunity Commission in 29 CFR Part 1613, shall apply to employment in...

  17. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... following body systems: Neurological; musculoskeletal; special sense organs; respiratory, including speech organs; cardiovascular; reproductive; digestive; genitourinary; hemic and lymphatic; skin; and endocrine... that term is defined for purposes of employment in 29 CFR 1613.702(f), which is made applicable to...

  18. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... impairments, cerebral palsy, epilepsy, muscular dystrophy, multiple sclerosis, cancer, heart disease, diabetes... that term is defined for purposes of employment in 29 CFR 1613.702(f), which is made applicable to...

  19. 15 CFR 8c.3 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... impairments, cerebral palsy, epilepsy, muscular dystrophy, multiple sclerosis, cancer, heart disease, diabetes... that term is defined for purposes of employment in 29 CFR 1613.702(f), which is made applicable to...

  20. 8-C N-ethyl-2-pyrrolidinone substituted flavan-3-ols as the marker compounds of Chinese dark teas formed in the post-fermentation process provide significant antioxidative activity.

    PubMed

    Wang, Weinan; Zhang, Liang; Wang, Shu; Shi, Shepo; Jiang, Yong; Li, Ning; Tu, Pengfei

    2014-01-01

    Phytochemical investigation of the aqueous extract of pu-erh tea afforded eight novel 8-C N-ethyl-2-pyrrolidinone substituted flavan-3-ols (puerins I-VIII) by (1)H, (13)C, two-dimensional nuclear magnetic resonance (NMR) and high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS) analysis. Comparative chemical analysis of green tea, black tea and Chinese dark teas confirmed that these compounds were the marker compounds of Chinese dark teas. Furthermore, fungal fermentation was indispensable for the biosynthesis of these novel compounds. Through single fungal fermentation, it was proved that catechins and theanine were the precursors of puerins I-VIII. HPLC-DAD-ESI/MS analysis elucidated the biosynthetic pathway for puerins I-VIII. Puerins I-IV have potential protective effects for the human micro-vascular endothelial cells (HMEC) injury induced by hydrogen dioxide compared to other tea polyphenols. 8-C N-ethyl-2-pyrrolidinone substituted flavan-3-ols could be used in the quality control and authentication of Chinese dark teas. PMID:24444972

  1. Evaluation of Cavity Collapse and Surface Crater Formation at the Norbo Underground Nuclear Test in U8c, Nevada Nuclear Security Site, and the Impact on Stability of the Ground Surface

    SciTech Connect

    Pawloski, G A

    2012-06-18

    Lawrence Livermore National Laboratory (LLNL) Containment Program performed a review of nuclear test-related data for the Norbo underground nuclear test in U8c to assist in evaluating this legacy site as a test bed for application technologies for use in On-Site Inspections (OSI) under the Comprehensive Nuclear Test Ban Treaty. This request is similar to one made for the Salut site in U8c (Pawloski, 2012b). Review of the Norbo site is complicated because the test first exhibited subsurface collapse, which was not unusual, but it then collapsed to the surface over one year later, which was unusual. Of particular interest is the stability of the ground surface above the Norbo detonation point. Proposed methods for on-site verification include radiological signatures, artifacts from nuclear testing activities, and imaging to identify alteration to the subsurface hydrogeology due to the nuclear detonation. Aviva Sussman from the Los Alamos National Laboratory (LANL) has also proposed work at this site. Both proposals require physical access at or near the ground surface of specific underground nuclear test locations at the Nevada Nuclear Security Site (NNSS), formerly the Nevada Test Site (NTS), and focus on possible activities such as visual observation, multispectral measurements, and shallow and deep geophysical surveys.

  2. MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.

    PubMed

    Wang, Youning; Li, Kexue; Chen, Liang; Zou, Yanmin; Liu, Haipei; Tian, Yinping; Li, Dongxiao; Wang, Rui; Zhao, Fang; Ferguson, Brett J; Gresshoff, Peter M; Li, Xia

    2015-07-01

    Legume root nodules convert atmospheric nitrogen gas into ammonium through symbiosis with a prokaryotic microsymbiont broadly called rhizobia. Auxin signaling is required for determinant nodule development; however, the molecular mechanism of auxin-mediated nodule formation remains largely unknown. Here, we show in soybean (Glycine max) that the microRNA miR167 acts as a positive regulator of lateral root organs, namely nodules and lateral roots. miR167c expression was up-regulated in the vasculature, pericycle, and cortex of soybean roots following inoculation with Bradyrhizobium japonicum strain USDA110 (the microsymbiont). It was found to positively regulate nodule numbers directly by repressing the target genes GmARF8a and GmARF8b (homologous genes of Arabidopsis [Arabidopsis thaliana] AtARF8 that encode auxin response factors). Moreover, the expression of miR167 and its targets was up- and down-regulated by auxin, respectively. The miR167-GmARF8 module also positively regulated nodulation efficiency under low microsymbiont density, a condition often associated with environmental stress. The regulatory role of miR167 on nodule initiation was dependent on the Nod factor receptor GmNFR1α, and it acts upstream of the nodulation-associated genes nodule inception, nodulation signaling pathway1, early nodulin40-1, NF-YA1 (previously known as HAEM activator protein2-1), and NF-YA2. miR167 also promoted lateral root numbers. Collectively, our findings establish a key role for the miR167-GmARF8 module in auxin-mediated nodule and lateral root formation in soybean. PMID:25941314

  3. MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development1[OPEN

    PubMed Central

    Wang, Youning; Li, Kexue; Chen, Liang; Zou, Yanmin; Tian, Yinping; Li, Dongxiao; Wang, Rui; Zhao, Fang; Ferguson, Brett J.; Gresshoff, Peter M.

    2015-01-01

    Legume root nodules convert atmospheric nitrogen gas into ammonium through symbiosis with a prokaryotic microsymbiont broadly called rhizobia. Auxin signaling is required for determinant nodule development; however, the molecular mechanism of auxin-mediated nodule formation remains largely unknown. Here, we show in soybean (Glycine max) that the microRNA miR167 acts as a positive regulator of lateral root organs, namely nodules and lateral roots. miR167c expression was up-regulated in the vasculature, pericycle, and cortex of soybean roots following inoculation with Bradyrhizobium japonicum strain USDA110 (the microsymbiont). It was found to positively regulate nodule numbers directly by repressing the target genes GmARF8a and GmARF8b (homologous genes of Arabidopsis [Arabidopsis thaliana] AtARF8 that encode auxin response factors). Moreover, the expression of miR167 and its targets was up- and down-regulated by auxin, respectively. The miR167-GmARF8 module also positively regulated nodulation efficiency under low microsymbiont density, a condition often associated with environmental stress. The regulatory role of miR167 on nodule initiation was dependent on the Nod factor receptor GmNFR1α, and it acts upstream of the nodulation-associated genes NODULE INCEPTION, NODULATION SIGNALING PATHWAY1, EARLY NODULIN40-1, NF-YA1 (previously known as HAEM ACTIVATOR PROTEIN2-1), and NF-YA2. miR167 also promoted lateral root numbers. Collectively, our findings establish a key role for the miR167-GmARF8 module in auxin-mediated nodule and lateral root formation in soybean. PMID:25941314

  4. MicroRNA167-Directed Regulation of the Auxin Response Factors GmARF8a and GmARF8b Is Required for Soybean Nodulation and Lateral Root Development.

    PubMed

    Wang, Youning; Li, Kexue; Chen, Liang; Zou, Yanmin; Liu, Haipei; Tian, Yinping; Li, Dongxiao; Wang, Rui; Zhao, Fang; Ferguson, Brett J; Gresshoff, Peter M; Li, Xia

    2015-07-01

    Legume root nodules convert atmospheric nitrogen gas into ammonium through symbiosis with a prokaryotic microsymbiont broadly called rhizobia. Auxin signaling is required for determinant nodule development; however, the molecular mechanism of auxin-mediated nodule formation remains largely unknown. Here, we show in soybean (Glycine max) that the microRNA miR167 acts as a positive regulator of lateral root organs, namely nodules and lateral roots. miR167c expression was up-regulated in the vasculature, pericycle, and cortex of soybean roots following inoculation with Bradyrhizobium japonicum strain USDA110 (the microsymbiont). It was found to positively regulate nodule numbers directly by repressing the target genes GmARF8a and GmARF8b (homologous genes of Arabidopsis [Arabidopsis thaliana] AtARF8 that encode auxin response factors). Moreover, the expression of miR167 and its targets was up- and down-regulated by auxin, respectively. The miR167-GmARF8 module also positively regulated nodulation efficiency under low microsymbiont density, a condition often associated with environmental stress. The regulatory role of miR167 on nodule initiation was dependent on the Nod factor receptor GmNFR1α, and it acts upstream of the nodulation-associated genes nodule inception, nodulation signaling pathway1, early nodulin40-1, NF-YA1 (previously known as HAEM activator protein2-1), and NF-YA2. miR167 also promoted lateral root numbers. Collectively, our findings establish a key role for the miR167-GmARF8 module in auxin-mediated nodule and lateral root formation in soybean.

  5. Kasatkinite, Ba2Ca8B5Si8O32(OH)3 · 6H2O6, a new mineral from the Bazhenovskoe deposit, the Central Urals, Russia

    NASA Astrophysics Data System (ADS)

    Pekov, I. V.; Chukanov, N. V.; Filinchuk, Ya. E.; Zadov, A. E.; Kononkova, N. N.; Epanchintsev, S. G.; Kaden, P.; Kutzer, A.; Göttlicher, J.

    2013-12-01

    A new mineral, kasatkinite, Ba2Ca8B5Si8O32(OH)3 · 6H2O, has been found at the Bazhenovskoe chrysotile asbestos deposit, the Central Urals, Russia in the cavities in rhodingite as a member of two assemblages: (l) on prehnite, with pectolite, calcite, and clinochlore; and (2) on grossular, with diopside and pectolite. Kasatkinite occurs as spherulites or bunches up to 3 mm in size, occasionally combined into crusts. Its individuals are acicular to hair-like, typically split, with a polygonal cross section, up to 0.5 mm (rarely, to 6 mm) in length and to 20 μm in thickness. They consist of numerous misoriented needle-shaped subindividuals up to several dozen μm long and no more than 1 μm thick. Kasatkinite individuals are transparent and colorless; its aggregates are snow white. The luster is vitreous or silky. No cleavage was observed; the fracture is uneven or splintery for aggregates. Individuals are flexible and elastic. The Mohs' hardness is 4-4.5. D meas = 2.95(5), D calc = 2.89 g/cm3. Kasatkinite is optically biaxial (+), α = 1.600(5), β = 1.603(2), γ = 1.626(2), 2 V meas = 30(20)°, 2 V calc = 40°. The IR spectrum is given. The 11B MAS NMR spectrum shows the presence of BO4 in the absence of BO3 groups. The chemical composition of kasatkinite (wt %; electron microprobe, H2O by gas chromatography) is as follows: 0.23 Na2O, 0.57 K2O, 28.94 CaO, 16.79 BaO, 11.57 B2O3, 0.28 Al2O3, 31.63 SiO2, 0.05 F, 9.05 H2O, -0.02 -O=F2; the total is 99.09. The empirical formula (calculated on the basis of O + F = 41 apfu, taking into account the TGA data) is: Na0.11K0.18Ba1.66Ca7.84B5.05Al0.08Si8.00O31.80(OH)3.06F0.04 · 6.10H2O. Kasatkinite is monoclinic, space group P21/ c, P2/ c, or Pc; the unit-cell dimensions are a = 5.745(3), b = 7.238(2), c = 20.79 (1) Å, β = 90.82(5)°, V = 864(1) Å3, Z = 1. The strongest reflections ( d Å- I[ hkl]) in the X-ray powder diffractions pattern are: 5.89-24[012], 3.48-2.1[006], 3.36-24[114]; 3.009-100[, 121, ], 2

  6. Alkyl nitrate formation from the reactions of C8-C14 n-alkanes with OH radicals in the presence of NO(x): measured yields with essential corrections for gas-wall partitioning.

    PubMed

    Yeh, Geoffrey K; Ziemann, Paul J

    2014-09-18

    In this study, C8-C14 n-alkanes were reacted with OH radicals in the presence of NO(x) in a Teflon film environmental chamber and isomer-specific yields of alkyl nitrates were determined using gas chromatography. Because results indicated significant losses of alkyl nitrates to chamber walls, gas-wall partitioning was investigated by monitoring the concentrations of a suite of synthesized alkyl nitrates added to the chamber. Gas-to-wall partitioning increased with increasing carbon number and with proximity of the nitrooxy group to the terminal carbon, with losses as high as 86%. The results were used to develop a structure-activity model to predict the effects of carbon number and isomer structure on gas-wall partitioning, which was used to correct the measured yields of alkyl nitrate isomers formed in chamber reactions. The resulting branching ratios for formation of secondary alkyl nitrates were similar for all isomers of a particular carbon number, and average values, which were almost identical to alkyl nitrate yields, were 0.219, 0.206, 0.254, 0.291, and 0.315 for reactions of n-octane, n-decane, n-dodecane, n-tridecane, and n-tetradecane, respectively. The increase in average branching ratios and alkyl nitrate yields with increasing carbon number to a plateau value of ∼0.30 at about C13-C14 is consistent with predictions of a previously developed model, indicating that the model is valid for alkane carbon numbers ≥C3.

  7. Alkyl nitrate formation from the reactions of C8-C14 n-alkanes with OH radicals in the presence of NO(x): measured yields with essential corrections for gas-wall partitioning.

    PubMed

    Yeh, Geoffrey K; Ziemann, Paul J

    2014-09-18

    In this study, C8-C14 n-alkanes were reacted with OH radicals in the presence of NO(x) in a Teflon film environmental chamber and isomer-specific yields of alkyl nitrates were determined using gas chromatography. Because results indicated significant losses of alkyl nitrates to chamber walls, gas-wall partitioning was investigated by monitoring the concentrations of a suite of synthesized alkyl nitrates added to the chamber. Gas-to-wall partitioning increased with increasing carbon number and with proximity of the nitrooxy group to the terminal carbon, with losses as high as 86%. The results were used to develop a structure-activity model to predict the effects of carbon number and isomer structure on gas-wall partitioning, which was used to correct the measured yields of alkyl nitrate isomers formed in chamber reactions. The resulting branching ratios for formation of secondary alkyl nitrates were similar for all isomers of a particular carbon number, and average values, which were almost identical to alkyl nitrate yields, were 0.219, 0.206, 0.254, 0.291, and 0.315 for reactions of n-octane, n-decane, n-dodecane, n-tridecane, and n-tetradecane, respectively. The increase in average branching ratios and alkyl nitrate yields with increasing carbon number to a plateau value of ∼0.30 at about C13-C14 is consistent with predictions of a previously developed model, indicating that the model is valid for alkane carbon numbers ≥C3. PMID:24654572

  8. Influence of the availability of iron during hypoxia on the genes associated with apoptotic activity and local iron metabolism in rat H9C2 cardiomyocytes and L6G8C5 skeletal myocytes.

    PubMed

    Dziegala, Magdalena; Kasztura, Monika; Kobak, Kamil; Bania, Jacek; Banasiak, Waldemar; Ponikowski, Piotr; Jankowska, Ewa A

    2016-10-01

    The differential availability of iron during hypoxia is presumed to affect the functioning of cardiac and skeletal myocytes. Rat H9C2 cardiomyocytes and L6G8C5 myocytes were cultured for 48 h in normoxic or hypoxic conditions at the optimal, reduced or increased iron concentration. The mRNA expression levels of markers of apoptosis [B‑cell lymphoma‑2 (Bcl2; inhibition) and Bcl‑2‑activated X protein (Bax; induction)], atrophy (Atrogin), glycolysis (pyruvate kinase 2; PKM2) and iron metabolism [transferrin receptor 1 (TfR1; iron importer), ferroportin 1 (FPN1; iron exporter), ferritin heavy chain (FTH; iron storage protein) and hepcidin (HAMP; iron regulator)] were determined using reverse transcription‑quantitative polymerase chain reaction, and cell viability was measured using an tetrazolium reduction assay. Cardiomyocytes and myocytes, when exposed to hypoxia, demonstrated an increased Bax/Bcl‑2 gene expression ratio (P<0.05). Additional deferoxamine (DFO) treatment resulted in further increases in Bax/Bcl‑2 in each cell type (P<0.001 each) and this was associated with the 15% loss in viability. The analogous alterations were observed in both cell types upon ammonium ferric citrate (AFC) treatment during hypoxia; however, the increased Bax/Bcl‑2 ratio and associated viability loss was lower compared with that in case of DFO treatment (P<0.05 each). Under hypoxic conditions, myocytes demonstrated an increased expression of PKM2 (P<0.01). Additional DFO treatment caused an increase in the mRNA expression levels of PKM2 and Atrogin‑1 (P<0.001 and P<0.05, respectively), whereas AFC treatment caused an increased mRNA expression of PKM2 (P<0.01) and accompanied decreased mRNA expression of Atrogin‑1 (P<0.05). The expression augmentation of PKM2 during hypoxia was greater upon low iron compared with that of ferric salt treatment (P<0.01). Both cell types upon DFO during hypoxia demonstrated the increased expression of TfR1

  9. Influence of the availability of iron during hypoxia on the genes associated with apoptotic activity and local iron metabolism in rat H9C2 cardiomyocytes and L6G8C5 skeletal myocytes.

    PubMed

    Dziegala, Magdalena; Kasztura, Monika; Kobak, Kamil; Bania, Jacek; Banasiak, Waldemar; Ponikowski, Piotr; Jankowska, Ewa A

    2016-10-01

    The differential availability of iron during hypoxia is presumed to affect the functioning of cardiac and skeletal myocytes. Rat H9C2 cardiomyocytes and L6G8C5 myocytes were cultured for 48 h in normoxic or hypoxic conditions at the optimal, reduced or increased iron concentration. The mRNA expression levels of markers of apoptosis [B‑cell lymphoma‑2 (Bcl2; inhibition) and Bcl‑2‑activated X protein (Bax; induction)], atrophy (Atrogin), glycolysis (pyruvate kinase 2; PKM2) and iron metabolism [transferrin receptor 1 (TfR1; iron importer), ferroportin 1 (FPN1; iron exporter), ferritin heavy chain (FTH; iron storage protein) and hepcidin (HAMP; iron regulator)] were determined using reverse transcription‑quantitative polymerase chain reaction, and cell viability was measured using an tetrazolium reduction assay. Cardiomyocytes and myocytes, when exposed to hypoxia, demonstrated an increased Bax/Bcl‑2 gene expression ratio (P<0.05). Additional deferoxamine (DFO) treatment resulted in further increases in Bax/Bcl‑2 in each cell type (P<0.001 each) and this was associated with the 15% loss in viability. The analogous alterations were observed in both cell types upon ammonium ferric citrate (AFC) treatment during hypoxia; however, the increased Bax/Bcl‑2 ratio and associated viability loss was lower compared with that in case of DFO treatment (P<0.05 each). Under hypoxic conditions, myocytes demonstrated an increased expression of PKM2 (P<0.01). Additional DFO treatment caused an increase in the mRNA expression levels of PKM2 and Atrogin‑1 (P<0.001 and P<0.05, respectively), whereas AFC treatment caused an increased mRNA expression of PKM2 (P<0.01) and accompanied decreased mRNA expression of Atrogin‑1 (P<0.05). The expression augmentation of PKM2 during hypoxia was greater upon low iron compared with that of ferric salt treatment (P<0.01). Both cell types upon DFO during hypoxia demonstrated the increased expression of TfR1

  10. 15 CFR 8b.23 - Housing provided by the recipient.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... of living accommodations is, as a whole, comparable to that of nonhandicapped students. (b) Other... of its students shall take such action as may be necessary to assure itself that such housing is, as a whole, made available in a manner that does not result in discrimination on the basis of handicap....

  11. 17 CFR 270.8b-2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... statements, means certified by an independent public or independent certified public accountant or accountants. (c) Charter. The term “charter” includes articles of incorporation, declaration of trust, articles of association or partnership, or any similar instrument, as amended, effecting (either with...

  12. 17 CFR 270.8b-2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... accountants. (c) Charter. The term “charter” includes articles of incorporation, declaration of trust... of the advisory board. (e) Fiscal year. The term “fiscal year” means the annual accounting period...

  13. 15 CFR 8b.20 - Admission and recruitment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...); (ii) admissions tests that are designed for persons with impaired sensory, manual, or speaking skills... are handicapped, Provided, That: (1) The recipient states clearly on any written questionnaire used for this purpose or makes clear orally, if no written questionnaire is used, that the...

  14. 15 CFR 8b.20 - Admission and recruitment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... speaking skills, the test results accurately reflect the applicant's aptitude or achievement level of..., manual, or speaking skills (except where those skills are the factors that the test purports to measure); (ii) admissions tests that are designed for persons with impaired sensory, manual, or speaking...

  15. Crystal structures of the novel hydrated borates Ba{sub 2}B{sub 5}O{sub 9}(OH), Sr{sub 2}B{sub 5}O{sub 9}(OH) and Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2}

    SciTech Connect

    McMillen, Colin; Heyward, Carla; Giesber, Henry; Kolis, Joseph

    2011-11-15

    Three novel hydrated borates Ba{sub 2}B{sub 5}O{sub 9}(OH) (1), Sr{sub 2}B{sub 5}O{sub 9}(OH) (2) and Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2} (3) have been synthesized hydrothermally and their structures determined. Compounds (1) and (2) are isostructural, crystallizing in space group P2{sub 1}/c and having lattice parameters of a=6.6330(13) A, b=8.6250(17) A, c=14.680(3) A, {beta}=93.46(3){sup o} and a=6.4970(13) A, b=8.4180(17) A, c=14.177(3) A, {beta}=94.35(3){sup o}, respectively. Compound (3) crystallizes in P-1 with lattice parameters of a=6.4684(13) A, b=8.4513(17) A, c=14.881(3) A, {alpha}=101.21(3){sup o}, {beta}=93.96(3){sup o}, {gamma}=90.67(3){sup o}. While similar in their axis lengths, (3) differs greatly in structure and formulation from (1) and (2). The structure of (1) and (2) is contrasted to that of the well-known mineral hilgardite (Ca{sub 2}B{sub 5}O{sub 9}Cl.H{sub 2}O). - Graphical abstract: Novel hydrated borate structures. Highlights: > Synthesis and crystal structures of three novel hydrated borates are reported. > Unique descriptive chemistry governing Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2} synthesis is discussed. > Structures of Ba{sub 2}B{sub 5}O{sub 9}(OH) and Sr{sub 2}B{sub 5}O{sub 9}(OH) are contrasted to Ca{sub 2}B{sub 5}O{sub 9}Cl.H{sub 2}O. > Structure of Li{sub 2}Sr{sub 8}B{sub 22}O{sub 41}(OH){sub 2} differs from Ba{sub 2}B{sub 5}O{sub 9}(OH) and Sr{sub 2}B{sub 5}O{sub 9}(OH).

  16. Shaped Sonic Boom Demonstration/Experiment Airborne Data

    NASA Technical Reports Server (NTRS)

    Haering ,Edward A., Jr.; Murray, James E.

    2004-01-01

    This viewgraph presentation reviews NASA's project to demonstrate that careful design of aircraft contour the resultant sonic boom can maintain a tailored shape, propagating through a real atmosphere down to ground level. The areas in covered in this presentation are: (1) Past airborne shock measurement efforts, (2) SR-71 Sonic Boom Propagation Experiment (3) F-5E Inlet Spillage Shock Measurement (4) Flight test approach (5) GPS data (6) Shaped Sonic Boom Demonstration (SSBD) Mach calibration (7) Super Blanik L-23 sailplane (8) Near-field probing (8a)Maneuvers (8b) Control Room Displays (8c) Pressure Instrumentation (8d) Signatures.

  17. F-8C adaptive control law refinement and software development

    NASA Technical Reports Server (NTRS)

    Hartmann, G. L.; Stein, G.

    1981-01-01

    An explicit adaptive control algorithm based on maximum likelihood estimation of parameters was designed. To avoid iterative calculations, the algorithm uses parallel channels of Kalman filters operating at fixed locations in parameter space. This algorithm was implemented in NASA/DFRC's Remotely Augmented Vehicle (RAV) facility. Real-time sensor outputs (rate gyro, accelerometer, surface position) are telemetered to a ground computer which sends new gain values to an on-board system. Ground test data and flight records were used to establish design values of noise statistics and to verify the ground-based adaptive software.

  18. 15 CFR 8c.10 - Self-evaluation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... handicaps or organizations representing individuals with handicaps, to participate in the self-evaluation...) A description of areas examined and any problems identified, and (2) A description of...

  19. The shape of the Sc2(μ2-S) unit trapped in C82: crystallographic, computational, and electrochemical studies of the isomers, Sc2(μ2-S)@C(s)(6)-C82 and Sc2(μ2-S)@C(3v)(8)-C82.

    PubMed

    Mercado, Brandon Q; Chen, Ning; Rodríguez-Fortea, Antonio; Mackey, Mary A; Stevenson, Steven; Echegoyen, Luis; Poblet, Josep M; Olmstead, Marilyn M; Balch, Alan L

    2011-05-01

    Single-crystal X-ray diffraction studies of Sc(2)(μ(2)-S)@C(s)(6)-C(82)·Ni(II)(OEP)·2C(6)H(6) and Sc(2)(μ(2)-S)@C(3v)(8)-C(82)·Ni(II)(OEP)·2C(6)H(6) reveal that both contain fully ordered fullerene cages. The crystallographic data for Sc(2)(μ(2)-S)@C(s)(6)-C(82)·Ni(II)(OEP)·2C(6)H(6) show two remarkable features: the presence of two slightly different cage sites and a fully ordered molecule Sc(2)(μ(2)-S)@C(s)(6)-C(82) in one of these sites. The Sc-S-Sc angles in Sc(2)(μ(2)-S)@C(s)(6)-C(82) (113.84(3)°) and Sc(2)(μ(2)-S)@C(3v)(8)-C(82) differ (97.34(13)°). This is the first case where the nature and structure of the fullerene cage isomer exerts a demonstrable effect on the geometry of the cluster contained within. Computational studies have shown that, among the nine isomers that follow the isolated pentagon rule for C(82), the cage stability changes markedly between 0 and 250 K, but the C(s)(6)-C(82) cage is preferred at temperatures ≥250 °C when using the energies obtained with the free encapsulated model (FEM). However, the C(3v)(8)-C(82) cage is preferred at temperatures ≥250 °C using the energies obtained by rigid rotor-harmonic oscillator (RRHO) approximation. These results corroborate the fact that both cages are observed and likely to trap the Sc(2)(μ(2)-S) cluster, whereas earlier FEM and RRHO calculations predicted only the C(s)(6)-C(82) cage is likely to trap the Sc(2)(μ(2)-O) cluster. We also compare the recently published electrochemistry of the sulfide-containing Sc(2)(μ(2)-S)@C(s)(6)-C(82) to that of corresponding oxide-containing Sc(2)(μ(2)-O)@C(s)(6)-C(82). PMID:21473581

  20. A novel technique for measurement of thermal rate constants and temperature dependences of dissociative recombination: CO2(+), CF3(+), N2O(+), C7H8(+), C7H7(+), C6H6(+), C6H5(+), C5H6(+), C4H4(+), and C3H3(+).

    PubMed

    Fournier, Joseph A; Shuman, Nicholas S; Melko, Joshua J; Ard, Shaun G; Viggiano, Albert A

    2013-04-21

    A novel technique using a flowing afterglow-Langmuir probe apparatus for measurement of temperature dependences of rate constants for dissociative recombination (DR) is presented. Low (~10(11) cm(-3)) concentrations of a neutral precursor are added to a noble gas∕electron afterglow plasma thermalized at 300-500 K. Charge exchange yields one or many cation species, each of which may undergo DR. Relative ion concentrations are monitored at a fixed reaction time while the initial plasma density is varied between 10(9) and 10(10) cm(-3). Modeling of the decrease in concentration of each cation relative to the non-recombining noble gas cation yields the rate constant for DR. The technique is applied to several species (O2(+), CO2(+), CF3(+), N2O(+)) with previously determined 300 K values, showing excellent agreement. The measurements of those species are extended to 500 K, with good agreement to literature values where they exist. Measurements are also made for a range of CnHm(+) (C7H7(+), C7H8(+), C5H6(+), C4H4(+), C6H5(+), C3H3(+), and C6H6(+)) derived from benzene and toluene neutral precursors. CnHm(+) DR rate constants vary from 8-12 × 10(-7) cm(3) s(-1) at 300 K with temperature dependences of approximately T(-0.7). Where prior measurements exist these results are in agreement, with the exception of C3H3(+) where the present results disagree with a previously reported flat temperature dependence. PMID:23614415

  1. Introducing New Technology into the Workplace: Retraining Issues and Strategies. Background Paper No. 8b.

    ERIC Educational Resources Information Center

    Kearsley, Greg

    Technological advances necessitate the continuous retraining of the work force. Three technologies are having greatest impact on the labor force: (1) the scope and depth of computer skills required by most jobs continue to expand; (2) robotics in manufacturing means that certain new jobs are more technical and require postsecondary education; and…

  2. Insights into Acetate Toxicity in Zymomonas mobilis 8b using Different Substrates

    DOE PAGES

    Yang, Shihui; Franden, M. A.; Brown, S. D.; Chou, Y. C.; Pienkos, P. T.; Zhang, Min

    2014-09-30

    The lignocellulosic biomass is a promising renewable feedstock for biofuel production. Acetate is one of the major inhibitors liberated from hemicelluloses during hydrolysis. Likewise, an understanding of the toxic effects of acetate on the fermentation microorganism and the efficient utilization of mixed sugars of glucose and xylose in the presence of hydrolysate inhibitors is crucial for economic biofuel production.

  3. W8...b4 IM, how did u rite??! Digital Writing in the Composition Classroom

    ERIC Educational Resources Information Center

    Partridge, Bryan

    2011-01-01

    From word processing computers, to mobile telephones, to the advent of the Internet, and finally to online communication venues like Instant Messenger (IM), the past four decades have brought an increasing prevalence of technology into our culture that is altering the English language. While decried by parents and lamented by teachers, these…

  4. 17 CFR 270.8b-33 - XBRL-Related Documents.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... electronic format as an exhibit to a filing on Form N-1A (§§ 239.15A and 274.11A of this chapter), Form N-CSR... separately identified. A registrant may submit such exhibit with, or in an amendment to, the Form N-CSR...

  5. Materials Data on Ba8B5N10F (SG:2) by Materials Project

    SciTech Connect

    Kristin Persson

    2014-07-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  6. 17 CFR 270.8b-16 - Amendments to registration statement.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... shareholders; (4) Any material changes in the principal risk factors associated with investment in the company... on Form N-SAR, as prescribed by rule 30b1-1 (17 CFR 270.30b1-1), shall amend the registration... company's prospectus by Item 10.1.e of Form N-2 (17 CFR 274.11a-1); (2) Any material changes in...

  7. International Communique. . . About Information, People, Places, Things. Printing Processes Issue P-8B.

    ERIC Educational Resources Information Center

    Peace Corps, Washington, DC. Information Collection and Exchange Div.

    Focusing on the production and utilization of printing processes in constructing effective visuals for teaching, this bulletin contains articles on the silk screening stencil process, use of a similar process with a portable mimeograph, and the hectograph process. The first article lists equipment needed to make a silk screen, steps in building…

  8. Carpentry II, 3-8B. Military Curriculum Materials for Vocational and Technical Education.

    ERIC Educational Resources Information Center

    Army Engineer School, Fort Belvoir, VA.

    This individualized, self-paced correspondence course in carpentry has been adapted from military curriculum materials for use in vocational and technical education programs. This second volume of a two-volume set is designed to present the theory portion of carpentry through the teaching of basic skills of frame construction. This Carpentry II…

  9. PROCEEDINGS: 1993 SO2 CONTROL SYMPOSIUM - VOLUME 4. SESSIONS 7, 8A, AND 8B

    EPA Science Inventory

    The report documents more than 100 presentations at the 1993 SO2 Control Symposium in Boston, MA, August 24-27, 1993. The presentations covered a wide range of topics: industry's strategies for dealing with Clean Air Act Amendments of 1990, including Phase I strategies, the emiss...

  10. Isomerization of the diphosphine ligand 3,4-bis(diphenylphosphino)-5-methoxy-2(5H)-furanone (bmf) at a triosmium cluster and P C bond cleavage in the unsaturated cluster 1,1-Os3(CO)9(bmf): Synthesis and X-ray diffraction structures of the isomeric Os3(CO)10(bmf) clusters and HOs3(CO)8( -C6H4)[ -PhPCC(Ph2P)CH(OMe)OC(O)

    SciTech Connect

    Kandala, Srikanth; Yang, Li; Campana, Charles F.; Nesterov, Vladimir; Richmond, Michael G.

    2010-07-01

    The labile cluster 1,2-Os3(CO)10(MeCN)2 (1) reacts with the chiral diphosphine ligand 3,4-bis(diphenylphosphino)-5-methoxy-2(5H)-furanone (bmf) to furnish 1,2-Os3(CO)10(bmf) (2a) in high yield. Heating cluster 2a over the temperature range 358 383 K under CO leads to isomerization of the bmf ligand and formation of the diphosphine-chelated cluster 1,1-Os3(CO)10(bmf) (2b) and an equilibrium mixture consisting of 2a and 2b in a 15:85 ratio. Extended thermolysis of an equilibrium mixture of Os3(CO)10(bmf) is accompanied by CO loss and ortho-metalation of an aryl ring to afford an inseparable mixture of three diastereomeric hydride clusters HOs3(CO)9(C29H23O3P2) (3a c). Thermolysis of HOs3(CO)9(C29H23O3P2) (3a c) in refluxing toluene leads to P C bond cleavage and formation of the benzyne-substituted clusters HOs3(CO)8( -C6H4)( -C23H19O3P2) (4a,b) as a 4:1 mixture of diastereomers. The unequivocal identity of the major benzyne-substituted cluster has been determined by X-ray diffraction analysis, where the activation of one of the phenyl groups situated to the furanone carbonyl group in the bmf ligand has been established. The isomerization and activation of the bmf ligand are contrasted with other Os3(CO)10(diphosphine) derivatives prepared by our groups.

  11. Quasi-three-coordinate iron and cobalt terphenoxide complexes {Ar(iPr8)OM(μ-O)}2 (Ar(iPr8) = C6H-2,6-(C6H2-2,4,6-(i)Pr3)2-3,5-(i)Pr2; M = Fe or Co) with M(III)2(μ-O)2 core structures and the peroxide dimer of 2-oxepinoxy relevant to benzene oxidation.

    PubMed

    Zhao, Pei; Lei, Hao; Ni, Chengbao; Guo, Jing-Dong; Kamali, Saeed; Fettinger, James C; Grandjean, Fernande; Long, Gary J; Nagase, Shigeru; Power, Philip P

    2015-09-21

    The bis(μ-oxo) dimeric complexes {Ar(iPr8)OM(μ-O)}2 (Ar(iPr8) = C6H-2,6-(C6H2-2,4,6-(i)Pr3)2-3,5-(i)Pr2; M = Fe (1), Co (2)) were prepared by oxidation of the M(I) half-sandwich complexes {Ar(iPr8)M(η(6)-arene)} (arene = benzene or toluene). Iron species 1 was prepared by reacting {Ar(iPr8)Fe(η(6)-benzene)} with N2O or O2, and cobalt species 2 was prepared by reacting {Ar(iPr8)Co(η(6)-toluene)} with O2. Both 1 and 2 were characterized by X-ray crystallography, UV-vis spectroscopy, magnetic measurements, and, in the case of 1, Mössbauer spectroscopy. The solid-state structures of both compounds reveal unique M2(μ-O)2 (M = Fe (1), Co(2)) cores with formally three-coordinate metal ions. The Fe···Fe separation in 1 bears a resemblance to that in the Fe2(μ-O)2 diamond core proposed for the methane monooxygenase intermediate Q. The structural differences between 1 and 2 are reflected in rather differing magnetic behavior. Compound 2 is thermally unstable, and its decomposition at room temperature resulted in the oxidation of the Ar(iPr8) ligand via oxygen insertion and addition to the central aryl ring of the terphenyl ligand to produce the 5,5'-peroxy-bis[4,6-(i)Pr2-3,7-bis(2,4,6-(i)Pr3-phenyl)oxepin-2(5H)-one] (3). The structure of the oxidized terphenyl species is closely related to that of a key intermediate proposed for the oxidation of benzene. PMID:26331405

  12. DC-9 flight demonstration program with refanned JT8D engines. Volume 2: Design and construction

    NASA Technical Reports Server (NTRS)

    1975-01-01

    The nacelle configuration selected for the DC-9 had a 1595.6 mm Refan length inlet and an 1811.8 mm exhaust duct. The inlet had 1234.4 mm of acoustic treatment and the tailpipe had 1305.5 mm of equivalent length acoustic treatment. The pylon was reduced in width from 425.5 mm to 204.5 mm. Fuselage frames and titanium skin panels in the area of the pylon were reinforced or replaced to support the higher loads and engine thrust. Experimental type tooling, fabrication and assembly were used on all hardware. The design is considered certifiable and representative of the hardware that would be built as retrofit kits.

  13. Spectral Re-Growth Reduction for CCSDS 8-D 8-PSK TCM

    NASA Technical Reports Server (NTRS)

    Borah, Deva K.

    2002-01-01

    This report presents a study on the CCSDS recommended 8-dimensional 8 PSK Trellis Coded Modulation (TCM) scheme. The important steps of the CCSDS scheme include: conversion of serial data into parallel form, differential encoding, convolutional encoding, constellation mapping, and filtering the 8-PSK symbols using the square root raised cosine (SRRC) pulses. The last step, namely the filtering of the 8 PSK symbols using SRRC pulses, significantly affects the bandwidth of the signal. If a nonlinear power amplifier is used, the SRRC filtered signal creates spectral regrowth. The purpose of this report is to investigate a technique, called the smooth phase interpolated keying (SPIK), that can provide an alternative to SRRC filtering so that good spectral as well as power efficiencies can be obtained with the CCSDS encoder. The results of this study show that the CCSDS encoder does not affect the spectral shape of the SRRC filtered signal or the SPIK signal. When a nonlinear traveling wave tube amplifier (TWTA) is used, the spectral performance of the SRRC signal degrades significantly while the spectral performance of SPIK remains unaffected. The degrading effect of a nonlinear solid state power amplifier (SSPA) on SRRC is found to be less than that due to a nonlinear TWTA. However, in both cases, the spectral performance of the SRRC modulated signal is worse than that of the SPIK signal. The bit error rate (BER) performance of the SRRC signal in a linear amplifier environment is about 2.5 dB better than that of the SPIK signal when both the receivers use algorithms of similar complexity. In a nonlinear TWTA environment, the SRRC signal requires accurate phase tracking since the TWTA introduces additional phase distortion. This problem does not arise with SPIK signal due to its constant envelope property. When a nonlinear amplifier is used, the SRRC method loses nearly 1 dB in the bit error rate performance. The SPIK signal does not lose any performance. Thus the performance gap between SRRC and SPIK reduces. The BER performance of SPIK can be improved even further by using a more optimal receiver. A similar optimal receiver for SRRC is quite complex since the amplifier distorts the pulse shape. However, this requires further investigation and is not covered in this report.

  14. 8D likelihood effective Higgs couplings extraction framework in h → 4 ℓ

    NASA Astrophysics Data System (ADS)

    Chen, Yi; Di Marco, Emanuele; Lykken, Joe; Spiropulu, Maria; Vega-Morales, Roberto; Xie, Si

    2015-01-01

    We present an overview of a comprehensive analysis framework aimed at performing direct extraction of all possible effective Higgs couplings to neutral electroweak gauge bosons in the decay to electrons and muons, the so called `golden channel'. Our framework is based primarily on a maximum likelihood method constructed from analytic expressions of the fully differential cross sections for h → 4 ℓ and for the dominant irreducible → 4 ℓ background, where 4 ℓ = 2 e2 μ, 4 e, 4 μ. Detector effects are included by an explicit convolution of these analytic expressions with the appropriate transfer function over all center of mass variables. Utilizing the full set of observables, we construct an unbinned detector-level likelihood which is continuous in the effective couplings. We consider possible ZZ, Zγ, and γγ couplings simultaneously, allowing for general CP odd/even admixtures. A broad overview is given of how the convolution is performed and we discuss the principles and theoretical basis of the framework. This framework can be used in a variety of ways to study Higgs couplings in the golden channel using data obtained at the LHC and other future colliders.

  15. Cepheids in open clusters: an 8D all-sky census

    NASA Astrophysics Data System (ADS)

    Anderson, Richard I.; Eyer, Laurent; Mowlavi, Nami

    2013-09-01

    Cepheids in Galactic open clusters (CCs) are of great importance as zero-point calibrators of the Galactic Cepheid period-luminosity relationship (PLR). We perform an eight-dimensional all-sky census that aims to identify new bona fide CCs and provides a ranking of membership confidence for known CC candidates according to membership probabilities. The probabilities are computed for combinations of known Galactic open clusters and classical Cepheid candidates, based on spatial, kinematic and population-specific membership constraints. Data employed in this analysis are taken largely from published literature and supplemented by a year-round observing programme on both hemispheres dedicated to determining systemic radial velocities of Cepheids. In total, we find 23 bona fide CCs, 5 of which are candidates identified for the first time, including an overtone-Cepheid member in NGC 129. We discuss a subset of CC candidates in detail, some of which have been previously mentioned in the literature. Our results indicate unlikely membership for seven Cepheids that have been previously discussed in terms of cluster membership. We furthermore revisit the Galactic PLR using our bona fide CC sample and obtain a result consistent with the recent calibration by Turner. However, our calibration remains limited mainly by cluster uncertainties and the small number of long-period calibrators. In the near future, Gaia will enable our study to be carried out in much greater detail and accuracy, thanks to data homogeneity and greater levels of completeness.

  16. 8D likelihood effective Higgs couplings extraction framework in h → 4ℓ

    SciTech Connect

    Chen, Yi; Di Marco, Emanuele; Lykken, Joe; Spiropulu, Maria; Vega-Morales, Roberto; Xie, Si

    2015-01-23

    We present an overview of a comprehensive analysis framework aimed at performing direct extraction of all possible effective Higgs couplings to neutral electroweak gauge bosons in the decay to electrons and muons, the so called ‘golden channel’. Our framework is based primarily on a maximum likelihood method constructed from analytic expressions of the fully differential cross sections for h → 4l and for the dominant irreducible $ q\\overline{q} $ → 4l background, where 4l = 2e2μ, 4e, 4μ. Detector effects are included by an explicit convolution of these analytic expressions with the appropriate transfer function over all center of mass variables. Utilizing the full set of observables, we construct an unbinned detector-level likelihood which is continuous in the effective couplings. We consider possible ZZ, Zγ, and γγ couplings simultaneously, allowing for general CP odd/even admixtures. A broad overview is given of how the convolution is performed and we discuss the principles and theoretical basis of the framework. This framework can be used in a variety of ways to study Higgs couplings in the golden channel using data obtained at the LHC and other future colliders.

  17. 8D likelihood effective Higgs couplings extraction framework in h → 4ℓ

    DOE PAGES

    Chen, Yi; Di Marco, Emanuele; Lykken, Joe; Spiropulu, Maria; Vega-Morales, Roberto; Xie, Si

    2015-01-23

    We present an overview of a comprehensive analysis framework aimed at performing direct extraction of all possible effective Higgs couplings to neutral electroweak gauge bosons in the decay to electrons and muons, the so called ‘golden channel’. Our framework is based primarily on a maximum likelihood method constructed from analytic expressions of the fully differential cross sections for h → 4l and for the dominant irreduciblemore » $$ q\\overline{q} $$ → 4l background, where 4l = 2e2μ, 4e, 4μ. Detector effects are included by an explicit convolution of these analytic expressions with the appropriate transfer function over all center of mass variables. Utilizing the full set of observables, we construct an unbinned detector-level likelihood which is continuous in the effective couplings. We consider possible ZZ, Zγ, and γγ couplings simultaneously, allowing for general CP odd/even admixtures. A broad overview is given of how the convolution is performed and we discuss the principles and theoretical basis of the framework. This framework can be used in a variety of ways to study Higgs couplings in the golden channel using data obtained at the LHC and other future colliders.« less

  18. Materials Data on VH8C10N3Cl4 (SG:14) by Materials Project

    SciTech Connect

    Kristin Persson

    2014-10-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  19. Materials Data on Eu8C4I9N7 (SG:156) by Materials Project

    SciTech Connect

    Kristin Persson

    2014-07-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  20. F-8C adaptive flight control extensions. [for maximum likelihood estimation

    NASA Technical Reports Server (NTRS)

    Stein, G.; Hartmann, G. L.

    1977-01-01

    An adaptive concept which combines gain-scheduled control laws with explicit maximum likelihood estimation (MLE) identification to provide the scheduling values is described. The MLE algorithm was improved by incorporating attitude data, estimating gust statistics for setting filter gains, and improving parameter tracking during changing flight conditions. A lateral MLE algorithm was designed to improve true air speed and angle of attack estimates during lateral maneuvers. Relationships between the pitch axis sensors inherent in the MLE design were examined and used for sensor failure detection. Design details and simulation performance are presented for each of the three areas investigated.

  1. Materials Data on TiH8C4NO10 (SG:180) by Materials Project

    SciTech Connect

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  2. Documentation of the Range 8C rehabilitation demonstration project at Hohenfels Training Area, West Germany

    SciTech Connect

    Zellmer, S.D.; Hinchman, R.R.; Carter, R.P.; Severinghaus, W.D.; Lacey, R.M.; Brent, J.J.

    1987-03-01

    Continued and intensive tactical training for the last 35 years at the Hohenfels Training Area (HTA), Federal Republic of Germany, has resulted in extensive environmental damage and reduced training realism. The US Corps of Engineers Construction Engineering Research Laboratory is developing an Integrated Training Area Management (ITAM) Program for the Seventh Army Training Command for use at HTA. Argonne National Laboratory was asked to assist in one element of the ITAM program, a training range rehabilitation demonstration project. The rehabilitation project was begun in 1986 on a 62-ha watershed that included about 16 ha of meadow with training damage typical of HTA. On the basis of amount of plant ground cover, type and degree of erosion, and soil properties, 10 rehabilitation prescriptions were developed to reestablish plant cover, control erosion, and improve training realism. Prescriptions were installed by a local contractor in September 1986. A monitoring program is under way to determine the effectiveness of this effort. Results and experience gained from this project will be used in the ITAM program and for rehabilitation training courses conducted at HTA.

  3. 17 CFR 240.8c-1 - Hypothecation of customers' securities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... amount of loans of such member, broker or dealer are payable and, in any event, before such member, broker or dealer on such day has obtained or increased any bank loan collateralized by securities carried... customer of such member, broker or dealer under a lien for a loan made to such member, broker or dealer;...

  4. Materials Data on H8C3SN(OF)3 (SG:14) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-04-22

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  5. Materials Data on CuH8C4O5 (SG:15) by Materials Project

    SciTech Connect

    Kristin Persson

    2014-07-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  6. Materials Data on TiH8C4NO10 (SG:181) by Materials Project

    SciTech Connect

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  7. Materials Data on InH8C4NO10 (SG:180) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  8. γ-Hydroxyethyl piperidine iminosugar and N-alkylated derivatives: a study of their activity as glycosidase inhibitors and as immunosuppressive agents.

    PubMed

    Markad, Pramod R; Sonawane, Dhiraj P; Ghosh, Sougata; Chopade, Balu A; Kumbhar, Navnath; Louat, Thierry; Herman, Jean; Waer, Mark; Herdewijn, Piet; Dhavale, Dilip D

    2014-11-01

    An efficient and practical strategy for the synthesis of (3R,4s,5S)-4-(2-hydroxyethyl) piperidine-3,4,5-triol and its N-alkyl derivatives 8a-f, starting from the D-glucose, is reported. The chiral pool methodology involves preparation of the C-3-allyl-α-D-ribofuranodialdose 10, which was converted to the C-5-amino derivative 11 by reductive amination. The presence of C-3-allyl group gives an easy access to the requisite hydroxyethyl substituted compound 13. Intramolecular reductive aminocyclization of C-5 amino group with C-1 aldehyde provided the γ-hydroxyethyl substituted piperidine iminosugar 8a that was N-alkylated to get N-alkyl derivatives 8b-f. Iminosugars 8a-f were screened against glycosidase enzymes. Amongst synthetic N-alkylated iminosugars, 8b and 8c were found to be α-galactosidase inhibitors while 8d and 8e were selective and moderate α-mannosidase inhibitors. In addition, immunomodulatory activity of compounds 8a-f was examined. These results were substantiated by molecular docking studies using AUTODOCK 4.2 programme.

  9. Sixth Grade Evaluation, Preliminary Study. Evaluation Report 8-B-1. Extended Pilot Trial of the Comprehensive School Mathematics Program.

    ERIC Educational Resources Information Center

    Marshall, Gail; Herbert, Martin

    The Comprehensive School Mathematics Program (CSMP) is a program of CEMREL, Inc., one of the national educational laboratories, and was funded by the National Institute of Education (NIE). Its major purpose is the development of curriculum materials for kindergarten through grade 6. An Extended Pilot Test was scheduled for spring 1982 for several…

  10. How Accurately Do We Know the Cross Section of the 7Be(p, {gamma})8B Reaction?

    SciTech Connect

    Gai, Moshe

    2006-03-13

    The 'world average' of the astrophysical cross section factor , S17(0), is driven by the Seattle result due to the very small quoted uncertainty, which we however demonstrate it to be an overestimated accuracy. We propose more realistic error bars for the Seattle results based on the published Seattle data. This leads to a an uncertainty of the measured slope of S17 and thus an uncertainty due to extrapolation that can be reasonably estimated to be {sub -3.0}{sup +0.0} eV-b.

  11. A 3.8 b.y. History of Bacterial Biofilms and Their Significance in the Search for Extraterrestrial Life

    NASA Technical Reports Server (NTRS)

    Westall, Frances; Steele, Andrew; Toporski, Jan; Walsh, Maud; Allen, Carlton; Guidry, Sean; McKay, David; Gibson, Everett; Chafetz, Henry

    2000-01-01

    Bacterial biofilms are almost ubiquitous in terrestrial environments, many similar to past or present Martian environments. Together with ToF-SIMS analysis of the in situ organics, fossil biofilms constitute reliable biomarkers.

  12. PoroTomo Subtask 3.8D - Raw Pressure Data from Boise Hydrogephysical Research Site (BHRS)

    DOE Data Explorer

    Lim, David

    2013-07-17

    Pressure data from a phreatic aquifer was collected in the summer of 2013 during Multi-frequency Oscillatory Hydraulic Tomography pumping tests. All tests were performed at the Boise Hydrogeophysical Research Site. The data will be inverted using a fast steady-periodic adjoint-based inverse code.

  13. Low speed wind tunnel flow field results for JT8D refan engines on the Boeing 727-200

    NASA Technical Reports Server (NTRS)

    Easterbrook, W. G.; Roberts, W. H.

    1974-01-01

    Low speed flow angularity results are presented showing flow direction at the nacelle locations on the Boeing 727-200. Flow angle probes (yawheads) were used for measurements at side and center inlet positions on the aft fuselage. A range of flap settings were tested with flap angles of 0 deg, 15 deg, and 40 deg selected for investigation.

  14. 76 FR 16526 - Airworthiness Directives; Pratt & Whitney JT8D-209, -217, -217A, -217C, and -219 Series Turbofan...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-24

    ...-209, -217, -217A, -217C, and -219 Series Turbofan Engines AGENCY: Federal Aviation Administration (FAA...-13948 (70 FR 3867, January 27, 2005). That AD applies to the specified products. The NPRM published in the Federal Register on July 1, 2010 (75 FR 38052). That NPRM proposed the same torque...

  15. 76 FR 8620 - Airworthiness Directives; Pratt & Whitney JT8D-209, -217, -217A, -217C, and -219 Turbofan Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-15

    ...-14242 (70 FR 71610, November 29, 2005). That AD applies to the specified products. The NPRM published in the Federal Register on August 18, 2010 (75 FR 50945). That NPRM proposed to modify the TLS of the... Executive Order 12866; (2) Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44...

  16. 75 FR 50945 - Airworthiness Directives; Pratt & Whitney JT8D-209, -217, -217A, -217C, and -219 Turbofan Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... complete Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477-78...-02, Amendment 39-14242 (70 FR 52004, September 1, 2005), to require revisions to the TLS of the... Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Would not have a...

  17. 75 FR 12968 - Airworthiness Directives; Pratt & Whitney JT8D-209, -217, -217C, and -219 Turbofan Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-18

    ... (62 FR 4902). ADDRESSES: You can get the service information identified in this AD from Pratt... proposed AD in the Federal Register on December 21, 2009 (74 FR 67831). That action proposed to require...'' under DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and (3) Will not have...

  18. Deletion of Huntington's disease-linked G8 (D4S10) locus in Wolf-Hirschhorn syndrome.

    PubMed

    Gusella, J F; Tanzi, R E; Bader, P I; Phelan, M C; Stevenson, R; Hayden, M R; Hofman, K J; Faryniarz, A G; Gibbons, K

    Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder characterized by progressive involuntary movements and dementia. The symptoms of the disease, although devastating in severity, do not usually appear until the third to fourth decade of life. The gene defect is highly penetrant, and results in the loss of neurones in the basal ganglia, globus pallidus, and more diffusely in the cortex. A DNA marker, G8 (or D4S10), is tightly linked to Huntington's disease and this gene has been localized to chromosome 4 (ref. 3). The discovery of this linkage marker raises the possibility of developing a presymptomatic test for the disorder, and of eventually isolating the disease gene based on its map position. We have now regionally localized the DNA marker G8 to the terminal band of the short arm of the chromosome, a region representing approximately 0.5% of the total human genome. The assignment was made by examining DNA from patients with Wolf-Hirschhorn syndrome, a birth defect resulting from partial heterozygous deletion of the short arm of chromosome 4.

  19. 75 FR 38052 - Airworthiness Directives; Pratt & Whitney JT8D-209, -217, -217A, -217C, and -219 Series Turbofan...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-01

    ... April 11, 2000 (65 FR 19477-78). Examining the AD Docket You may examine the AD docket on the Internet... 39 by superseding AD 2005-02- 03, Amendment 39-13948 (70 FR 3867, January 27, 2005). That AD requires... Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Would not have a...

  20. University Opinion Poll 8C, Fall 1973. Research Bulletin, University of Minnesota, V14 N8 January 28, 1974.

    ERIC Educational Resources Information Center

    Barnhart, William

    The University Opinion Poll conducted a survey to obtain an estimate of student and staff opinion toward alcohol on campus. Four hundred seventy students and staff, 77% of a random sample of all students and staff at the University of Minnesota, were contacted for their opinions. Most respondents favor having alcoholic beverages on campus and…

  1. Art Teaching Guides: Modeling with Clay, Kindergarten-Grade 6. Curriculum Bulletin No. 8c, 1968-69 Series.

    ERIC Educational Resources Information Center

    New York City Board of Education, Brooklyn, NY. Bureau of Curriculum Development.

    This guide offers teaching suggestions for introducing young children to the pleasure of handling clay, for guiding third and fourth grade children in shaping clay into many forms, and for instructing fifth and sixth grade children in the molding, glazing and firing of clay. Sections for each grade (K-6) include discussions of (1) materials and…

  2. Materials Data on BaH8C6(N3O4)2 (SG:2) by Materials Project

    SciTech Connect

    Kristin Persson

    2014-07-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  3. Ground wave emergency network environmental assessment for northwestern Colorado relay node site number RN 8C924CO

    NASA Astrophysics Data System (ADS)

    1993-02-01

    The Ground Wave Emergency Network (GWEN) is a radio communication system designed to relay emergency messages between strategic military areas in the continental United States. The system is immune to the effects of high-altitude electromagnetic pulse (HEMP) energy surges caused by nuclear bursts in the ionosphere that would disrupt conventional communications equipment such as telephones and shortwave radios. A failure of such equipment would prevent timely communications among top military and civilian leaders and strategic Air Force locations and prevent U.S. assessment and retaliation during an attack. GWEN is an essential part of a defense modernization program to upgrade and improve our nation's communications system, thereby strengthening deterrence. The GWEN system consists of a network of relay nodes, receive-only stations, and input/output stations. Each relay node, such as the one proposed in southern Nevada consists of a guyed radio tower facility similar to those used by commercial AM broadcast transmitters.

  4. Cobalt complexes with cinchonidine and quinidine: effect of C8/C9 stereochemistry and 6'-substitution on intermolecular interactions.

    PubMed

    Skórska, Agnieszka; Stadnicka, Katarzyna; Oleksyn, Barbara J

    2005-02-01

    The title compounds, (cinchonidinium)trichlorocobalt(II) [(C(19)H(23)ON(2))CoCl(3)] (CoCdn) and (quinidinium)trichlorocobalt(II) [(C(20)H(25)O(2)N(2))CoCl(3)] (CoQd), are zwitterions that differ in absolute configuration and conformation. In both complexes, the sp(3) nitrogen of quinuclidine is protonated, whereas the sp(2) nitrogen of quinoline is linked to the Co(II) atom, which coordinates three chlorine atoms in distorted tetrahedral geometry. The mutual orientations of the quinoline and quinuclidine moieties in CoCdn and CoQd differ significantly because of different hydrogen bonding involving the hydroxyl group. In both complexes, the quinuclidine NH groups and hydroxyl groups are hydrogen-bond donors to the chlorine atoms of Co(II) tetrahedra. In CoQd the hydrogen bonding leads to formation of a nine-membered ring consisting of Co, two chlorines, and a fragment of the quinidine molecule. A comparison of the crystal structures of four Cinchona alkaloid complexes with trichlorocobalt(II) shows that their space groups are determined by the absolute configuration of the alkaloid, whereas the hydrogen-bonding pattern is mainly affected by the substituent in the quinoline ring, i.e., by hydrogen or methoxyl group.

  5. Materials Data on ZnH8C4(NO3)2 (SG:52) by Materials Project

    SciTech Connect

    Kristin Persson

    2014-07-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  6. Materials Data on ZnH8C4(N4Cl)2 (SG:14) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  7. Materials Data on CrH8C4(S2N3)2 (SG:2) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-03-28

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  8. Materials Data on FeH8C4(S2N3)2 (SG:2) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-04-23

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  9. Materials Data on MgH8C4(N3O2)2 (SG:14) by Materials Project

    SciTech Connect

    Kristin Persson

    2014-07-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  10. Materials Data on CdH8C4(S2N3)2 (SG:2) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-03-28

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  11. Materials Data on CuH8C4(NO3)2 (SG:14) by Materials Project

    SciTech Connect

    Kristin Persson

    2015-01-27

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  12. Materials Data on InH8C8N4Cl7 (SG:18) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2014-10-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  13. G6PD haplotypes spanning Xq28 from F8C to red/green color vision

    SciTech Connect

    Filosa, S.; Lania, G.; Martini, G. ); Brancati, C.; Tagarelli, A. ); Calabro, V. Hammersmith Hospital, London ); Vulliamy, T.J.; Luzzatto, L. )

    1993-07-01

    The most telomeric region of the human X chromosome within band Xq28 consists of a gene-rich region of about 3 Mb which contains the genes for coagulation factor VIIIc, glucose-6-phosphate dehydrogenase (G6PD), and red/green color vision. The authors have studied five polymorphic sites from this region, in a sample of normal people from the Cosenza province of Southern Italy. These sites, which span a distance of some 350 kb, are in strong linkage disequilibrium. Of the 32 possible haplotypes only 10 were found, and 4 of these account for 80% of all X chromosomes analyzed. In addition, they found that all G6PD-deficient people with the G6PD Mediterranean mutation belong to only two haplotypes. One of these (Med 1) is found only within a small subregion of the area investigated, west of the Appennine mountain range. Most remarkably, all Med 1 G6PD-deficient individuals also had red/green color blindness. The more frequent haplotype (Med 2) is the same in Calabria and in Sardinia, where it accounts for about 90% of the G6PD Mediterranean mutations, despite the fact that gene flow between the populations of Sardinia and Southern Italy must have been limited. These data do not enable determination of whether the two types of G6PD Mediterranean have arisen through two separate identical mutational events or through a single mutational event followed by recombination. However, the data indicate relatively little recombination over an extended region of the X chromosome and they suggest that the G6PD Mediterranean mutation is recent by comparison to the other polymorphisms investigated. 44 refs., 4 figs., 5 tabs.

  14. Antimutagenic Compounds of White Shrimp (Litopenaeus vannamei): Isolation and Structural Elucidation.

    PubMed

    López-Saiz, Carmen-María; Hernández, Javier; Cinco-Moroyoqui, Francisco-Javier; Velázquez, Carlos; Ocaño-Higuera, Víctor-Manuel; Plascencia-Jatomea, Maribel; Robles-Sánchez, Maribel; Machi-Lara, Lorena; Burgos-Hernández, Armando

    2016-01-01

    According to the World Health Organization, cancer is the main cause of mortality worldwide; thus, the search of chemopreventive compounds to prevent the disease has become a priority. White shrimp (Litopenaeus vannamei) has been reported as a source of compounds with chemopreventive activities. In this study, shrimp lipids were extracted and then fractionated in order to isolate those compounds responsible for the antimutagenic activity. The antimutagenic activity was assessed by the inhibition of the mutagenic effect of aflatoxin B1 on TA98 and TA100 Salmonella tester strains using the Ames test. Methanolic fraction was responsible for the highest antimutagenic activity (95.6 and 95.9% for TA98 and TA100, resp.) and was further separated into fifteen different subfractions (M1-M15). Fraction M8 exerted the highest inhibition of AFB1 mutation (96.5 and 101.6% for TA98 and TA100, resp.) and, after further fractionation, four subfractions M8a, M8b, M8c, and M8d were obtained. Data from (1)H and (13)C NMR, and mass spectrometry analysis of fraction M8a (the one with the highest antimutagenic activity), suggest that the compound responsible for its antimutagenicity is an apocarotenoid.

  15. Pyrimidine-5-carbonitriles II: synthesis and antimicrobial activity of novel 6-alkyl-2,4-disubstituted pyrimidine-5-carbonitriles.

    PubMed

    Al-Abdullah, E S; Al-Turkistani, A A; Al-Deeb, O A; El-Brollosy, N R; Habib, E E; El-Emam, A A

    2014-01-01

    New series of 6-alkyl-2,4-disubstituted pyrimidine-5-carbonitriles namely, 6-alkyl-2-thiouracil-5-carbonitriles 4c,d, 6-alkyl-2-arylmethylsulfanyl-3,4-dihydro-4-oxopyrimidine-5-carbonitriles 5a-p, 6-alkyl-2-(2-methoxyethylsulfanyl)-3,4-dihydro-4-oxopyrimidine-5-carbonitriles 6a-d, 6-alkyl-2-benzyloxymethylsulfanyl-3,4-dihydro-4-oxopyrimidine-5-carbonitriles 7a-c, 6-alkyl-2-(5-nitrofuran-2-ylmethylsulfanyl)-3,4-dihydro-4-oxopyrimidine-5-carbonitriles 8a-d, 6-alkyl-4-arylthio-2-(benzylsulfanyl)pyrimidine-5-carbonitriles 10a, b and 2-benzylsulfanyl-4-[4-(2-methoxyphenyl)-1-piperazinyl]-6-pentylpyrimidine-5-carbonitrile 11, were synthesized and tested for in vitro activities against a panel of Gram-positive and Gram-negative bacteria and the yeast-like pathogenic fungus Candida albicans. Compounds 4d, 5b, 5c, 5d, 5e, 5f, 5g, 5h, 5i, 5j, 5k, 5 l, 5p, 7a, 7b, 7c, 8a, 8b, 8c, 8d and 11 -displayed marked antibacterial activity particularly against the tested Gram-positive bacteria. Meanwhile, none of these compounds were proved to be active against Candida albicans.

  16. Synthesis and biological evaluation of 4-alkoxy-6,9-dichloro[1,2,4]triazolo[4,3-a]quinoxalines as inhibitors of TNF-α and IL-6.

    PubMed

    Guirado, Antonio; López Sánchez, José I; Ruiz-Alcaraz, Antonio J; Bautista, Delia; Gálvez, Jesús

    2012-08-01

    An efficient synthetic method for previously unattainable 4-alkoxy-6,9-dichloro[1,2,4]triazolo[4,3-a]quinoxalines has been established. Reactions between 5,8-dichloro-2,3-dicyanoquinoxaline and alcohols in the presence of triethylamine led to 3-alkoxy-5,8-dichloro-2-cyanoquinoxalines in high to quantitative yields. These compounds were treated with hydrazine giving 3-alkoxy-5,8-dichloro-2-hydrazinoquinoxalines in near quantitative yields, that reacted with triethyl orthoformate to provide the title compounds in high yields. The molecular structure of a member of this family of compounds: 6,9-dichloro-4-ethoxy[1,2,4]triazolo[4,3-a]quinoxaline, was determined by X-ray crystallography. The series of compounds synthesized were evaluated for their potential anti-inflammatory activity as inhibitors of the pro-inflammatory cytokines TNF-α and IL-6. Compounds 8e, 8a, 8b and 8g presented simultaneously good levels of inhibition of both cytokines being compound 8e the most concomitantly potent one. Compounds 8d, 8f and 8h specifically inhibited IL-6 with no significant inhibition of TNF-α. Compound 8c was not significantly active upon TNF-α, and showed no activity upon IL-6.

  17. Antimutagenic Compounds of White Shrimp (Litopenaeus vannamei): Isolation and Structural Elucidation

    PubMed Central

    López-Saiz, Carmen-María; Hernández, Javier; Cinco-Moroyoqui, Francisco-Javier; Velázquez, Carlos; Ocaño-Higuera, Víctor-Manuel; Plascencia-Jatomea, Maribel; Robles-Sánchez, Maribel; Machi-Lara, Lorena; Burgos-Hernández, Armando

    2016-01-01

    According to the World Health Organization, cancer is the main cause of mortality worldwide; thus, the search of chemopreventive compounds to prevent the disease has become a priority. White shrimp (Litopenaeus vannamei) has been reported as a source of compounds with chemopreventive activities. In this study, shrimp lipids were extracted and then fractionated in order to isolate those compounds responsible for the antimutagenic activity. The antimutagenic activity was assessed by the inhibition of the mutagenic effect of aflatoxin B1 on TA98 and TA100 Salmonella tester strains using the Ames test. Methanolic fraction was responsible for the highest antimutagenic activity (95.6 and 95.9% for TA98 and TA100, resp.) and was further separated into fifteen different subfractions (M1–M15). Fraction M8 exerted the highest inhibition of AFB1 mutation (96.5 and 101.6% for TA98 and TA100, resp.) and, after further fractionation, four subfractions M8a, M8b, M8c, and M8d were obtained. Data from 1H and 13C NMR, and mass spectrometry analysis of fraction M8a (the one with the highest antimutagenic activity), suggest that the compound responsible for its antimutagenicity is an apocarotenoid. PMID:27006678

  18. Hydrophobic substituents increase the potency of salacinol, a potent α-glucosidase inhibitor from Ayurvedic traditional medicine 'Salacia'.

    PubMed

    Tanabe, Genzoh; Xie, Weijia; Balakishan, Gorre; Amer, Mumen F A; Tsutsui, Nozomi; Takemura, Haruka; Nakamura, Shinya; Akaki, Junji; Ninomiya, Kiyofumi; Morikawa, Toshio; Nakanishi, Isao; Muraoka, Osamu

    2016-08-15

    Using an in silico method, seven analogs bearing hydrophobic substituents (8a: Me, 8b: Et, 8c: n-Pent, 8d: n-Hept, 8e: n-Tridec, 8f: isoBu and 8g: neoPent) at the 3'-O-position in salacinol (1), a highly potent natural α-glucosidase inhibitor from Ayurvedic traditional medicine 'Salacia', were designed and synthesized. In order to verify the computational SAR assessments, their α-glucosidase inhibitory activities were evaluated in vitro. All analogs (8a-8g) exhibited an equal or considerably higher level of inhibitory activity against rat small intestinal α-glucosidases compared with the original sulfonate (1), and were as potent as or higher in potency than the clinically used anti-diabetics, voglibose, acarbose or miglitol. Their activities against human maltase exhibited good relationships to the results obtained with enzymes of rat origin. Among the designed compounds, the one with a 3'-O-neopentyl moiety (8g) was most potent, with an approximately ten fold increase in activity against human maltase compared to 1. PMID:27325449

  19. Antimutagenic Compounds of White Shrimp (Litopenaeus vannamei): Isolation and Structural Elucidation.

    PubMed

    López-Saiz, Carmen-María; Hernández, Javier; Cinco-Moroyoqui, Francisco-Javier; Velázquez, Carlos; Ocaño-Higuera, Víctor-Manuel; Plascencia-Jatomea, Maribel; Robles-Sánchez, Maribel; Machi-Lara, Lorena; Burgos-Hernández, Armando

    2016-01-01

    According to the World Health Organization, cancer is the main cause of mortality worldwide; thus, the search of chemopreventive compounds to prevent the disease has become a priority. White shrimp (Litopenaeus vannamei) has been reported as a source of compounds with chemopreventive activities. In this study, shrimp lipids were extracted and then fractionated in order to isolate those compounds responsible for the antimutagenic activity. The antimutagenic activity was assessed by the inhibition of the mutagenic effect of aflatoxin B1 on TA98 and TA100 Salmonella tester strains using the Ames test. Methanolic fraction was responsible for the highest antimutagenic activity (95.6 and 95.9% for TA98 and TA100, resp.) and was further separated into fifteen different subfractions (M1-M15). Fraction M8 exerted the highest inhibition of AFB1 mutation (96.5 and 101.6% for TA98 and TA100, resp.) and, after further fractionation, four subfractions M8a, M8b, M8c, and M8d were obtained. Data from (1)H and (13)C NMR, and mass spectrometry analysis of fraction M8a (the one with the highest antimutagenic activity), suggest that the compound responsible for its antimutagenicity is an apocarotenoid. PMID:27006678

  20. Molecular epidemiology of measles in India, 2005-2010.

    PubMed

    Wairagkar, Niteen; Chowdhury, Deepika; Vaidya, Sunil; Sikchi, Sarika; Shaikh, Naseem; Hungund, Laxman; Tomar, R S; Biswas, D; Yadav, K; Mahanta, J; Das, V N R; Yergolkar, Prasanna; Gunasekaran, P; Raja, D; Jadi, R; Ramamurty, Nalini; Mishra, A C

    2011-07-01

    Measles is a childhood disease that causes great morbidity and mortality in India and worldwide. Because measles surveillance in India is in its infancy, there is a paucity of countrywide data on circulating Measles virus genotypes. This study was conducted in 21 of 28 States and 2 of 7 Union Territories of India by MeaslesNetIndia, a national network of 27 centers and sentinel practitioners. MeaslesNetIndia investigated 52 measles outbreaks in geographically representative areas from 2005 through June 2010. All outbreaks were serologically confirmed by detection of antimeasles virus immunoglobulin M (IgM) antibodies in serum or oral fluid samples. Molecular studies, using World Health Organization (WHO)-recommended protocols obtained 203 N-gene, 40 H-gene, and 4 M-gene sequences during this period. Measles genotypes D4, D7, and D8 were found to be circulating in various parts of India during the study period. Further phylogenetic analysis revealed 4 lineages of Indian D8 genotypes: D8a, D8b, D8c, and D8d. This study generated a large, countrywide sequence database that can form the baseline for future molecular studies on measles virus transmission pathways in India. This study has created support and capabilities for countrywide measles molecular surveillance that must be carried forward.

  1. A CPG ISLAND AT THE PROMOTER OF THE PDE8B GENE IS METHYLATED IN PLACENTA AND HYDATIDIFORM MOLES, BUT NOT IN CONTROL DNA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Objective: We used a genome-wide CpG methylation screen, restriction landmark genome scanning (RLGS) to identify CpG islands that have altered methylation in complete hydatidiform moles (CHM), compared to control genomic DNA. Because CHM are diploid, but of uniparental parental inheritance and uniq...

  2. STEALTH: a Lagrange explicit finite-difference code for solid, structural, and thermohydraulic analysis. Volume 8B. STEALTH/WHAMSE: a 3-D fluid-structure interaction code

    SciTech Connect

    Not Available

    1984-10-01

    STEALTH is a family of computer codes that can be used to calculate a variety of physical processes in which the dynamic behavior of a continuum is involved. The version of STEALTH described in this volume is designed for calculations of fluid-structure interaction. This version of the program consists of a hydrodynamic version of STEALTH which has been coupled to a finite-element code, WHAMSE. STEALTH computes the transient response of the fluid continuum, while WHAMSE computes the transient response of shell and beam structures under external fluid loadings. The coupling between STEALTH and WHAMSE is performed during each cycle or step of a calculation. Separate calculations of fluid response and structure response are avoided, thereby giving a more accurate model of the dynamic coupling between fluid and structure. This volume provides the theoretical background, the finite-difference equations, the finite-element equations, a discussion of several sample problems, a listing of the input decks for the sample problems, a programmer's manual and a description of the input records for the STEALTH/WHAMSE computer program.

  3. A 120nW 8b Sub-ranging SAR ADC with Signal-Dependent Charge Recycling for Biomedical Applications

    PubMed Central

    Jeong, Seokhyeon; Jung, Wanyeong; Jeon, Dongsuk; Berenfeld, Omer; Oral, Hakan; Kruger, Grant; Blaauw, David; Sylvester, Dennis

    2015-01-01

    We present an 8-bit sub-ranging SAR ADC designed for bursty signals having long time periods with small code spread. A modified capacitive-DAC (CDAC) saves previous sample’s MSB voltage and reuses it throughout subsequent conversions. This prevents unnecessary switching of large MSB capacitors as well as conversion cycles, reducing energy consumed in the comparator and digital logic and yielding total energy savings of 2.6×. In 0.18μm CMOS, the ADC consumes 120nW at 0.6V and 100kS/s with 46.9dB SNDR. PMID:26855850

  4. Pesticide Applicator Training Manual, Category 8B: Mosquito Control for New Jersey. A Training Program for the Certification of Commercial Pesticide Applicators, and Study Questions.

    ERIC Educational Resources Information Center

    Schulze, Terry L., Ed.; Kriner, Ray R., Ed.

    This training manual provides information needed to meet the mimimum EPA standards for certification as a commercial applicator of pesticides in the mosquito control category. The text discusses the aspects of mosquito biology and control by biological, mechanical, and integrated measures. A study guide with sample and study questions is included.…

  5. Synthesis of Key Points from the OSEP Early Childhood Transition FAQ (SPP/APR Indicators C-8, B-11 and B-12)

    ERIC Educational Resources Information Center

    Diefendorf, M.; Henson, J.; Lucas, A.; Whaley, K.

    2010-01-01

    This document is a synthesis of the key points provided in the Office of Special Education Programs (OSEP) Early Childhood Transition FAQs: SPP/APR indicators C-8 and B-12 released on December 1, 2009. It was developed to assist states with the implementation of effective transition policies and practices. The following is included: (1) Transition…

  6. KELT-8b: A Highly Inflated Transiting Hot Jupiter and a New Technique for Extracting High-precision Radial Velocities from Noisy Spectra

    NASA Astrophysics Data System (ADS)

    Fulton, Benjamin J.; Collins, Karen A.; Gaudi, B. Scott; Stassun, Keivan G.; Pepper, Joshua; Beatty, Thomas G.; Siverd, Robert J.; Penev, Kaloyan; Howard, Andrew W.; Baranec, Christoph; Corfini, Giorgio; Eastman, Jason D.; Gregorio, Joao; Law, Nicholas M.; Lund, Michael B.; Oberst, Thomas E.; Penny, Matthew T.; Riddle, Reed; Rodriguez, Joseph E.; Stevens, Daniel J.; Zambelli, Roberto; Ziegler, Carl; Bieryla, Allyson; D'Ago, Giuseppe; DePoy, Darren L.; Jensen, Eric L. N.; Kielkopf, John F.; Latham, David W.; Manner, Mark; Marshall, Jennifer; McLeod, Kim K.; Reed, Phillip A.

    2015-09-01

    We announce the discovery of a highly inflated transiting hot Jupiter by the KELT-North survey. A global analysis including constraints from isochrones indicates that the V = 10.8 host star (HD 343246) is a mildly evolved, G dwarf with {T}{eff}={5754}-55+54 K, {log} g={4.078}-0.054+0.049, [{Fe}/{{H}}]=0.272+/- 0.038, an inferred mass {M}*={1.211}-0.066+0.078 {M}⊙ , and radius {R}*={1.67}-0.12+0.14 {R}⊙ . The planetary companion has a mass {M}{{P}}={0.867}-0.061+0.065 MJ, radius {R}{{P}}={1.86}-0.16+0.18 RJ, surface gravity {log} {g}{{P}}={2.793}-0.075+0.072, and density {ρ }{{P}}={0.167}-0.038+0.047 g cm-3. The planet is on a roughly circular orbit with semimajor axis a={0.04571}-0.00084+0.00096 AU and eccentricity e={0.035}-0.025+0.050. The best-fit linear ephemeris is {T}0=2456883.4803+/- 0.0007 {{BJD}}{TDB} and P=3.24406+/- 0.00016 days. This planet is one of the most inflated of all known transiting exoplanets, making it one of the few members of a class of extremely low density, highly irradiated gas giants. The low stellar {log} g and large implied radius are supported by stellar density constraints from follow-up light curves, as well as an evolutionary and space motion analysis. We also develop a new technique to extract high-precision radial velocities from noisy spectra that reduces the observing time needed to confirm transiting planet candidates. This planet boasts deep transits of a bright star, a large inferred atmospheric scale height, and a high equilibrium temperature of {T}{eq}={1675}-55+61 K, assuming zero albedo and perfect heat redistribution, making it one of the best targets for future atmospheric characterization studies.

  7. 40 CFR Table 1 to Subpart Ddd of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart DDD of Part 63

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Subpart DDD does not require CMS performance specifications. 63.8(a)(3) No . 63.8(a)(4) Yes 63.8(b... does not require COMS or CMS performance specifications. 63.8(d) Quality Control No Subpart DDD does... Subpart DDD does not require CMS performance specifications. 63.10(c)(7)-(c)(8) Yes 63.10(c)(9) No ....

  8. 75 FR 27972 - Airworthiness Directives; Pratt & Whitney JT8D-9, -9A, -11, -15, -17, and -17R Turbofan Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-19

    .... This proposed AD would require overhauling fan blade leading edges at the first shop visit after 4,000 cycles-in-service (CIS) since the last total fan blade overhaul was performed. This proposed AD results from reports of failed fan blades. We are proposing this AD to prevent high-cycle fatigue cracking...

  9. Wind tunnel test of model target thrust reversers for the Pratt and Whitney aircraft JT8D-100 series engines installed on a 727-200 airplane

    NASA Technical Reports Server (NTRS)

    Hambly, D.

    1974-01-01

    The results of a low speed wind tunnel test of 0.046 scale model target thrust reversers installed on a 727-200 model airplane are presented. The full airplane model was mounted on a force balance, except for the nacelles and thrust reversers, which were independently mounted and isolated from it. The installation had the capability of simulating the inlet airflows and of supplying the correct proportions of primary and secondary air to the nozzles. The objectives of the test were to assess the compatibility of the thrust reversers target door design with the engine and airplane. The following measurements were made: hot gas ingestion at the nacelle inlets; model lift, drag, and pitching moment; hot gas impingement on the airplane structure; and qualitative assessment of the rudder effectiveness. The major parameters controlling hot gas ingestion were found to be thrust reverser orientation, engine power setting, and the lip height of the bottom thrust reverser doors on the side nacelles. The thrust reversers tended to increase the model lift, decrease the drag, and decrease the pitching moment.

  10. Art Teaching Guides: Using Cloth and Trimmings, Yarns and Fibers, Grades 2-6. Curriculum Bulletin No. 8d, 1968-69 Series.

    ERIC Educational Resources Information Center

    New York City Board of Education, Brooklyn, NY. Bureau of Curriculum Development.

    Creating with fabrics, yarns, and fibers--from early basic explorations in handling an assortment of cloths and trimmings, through experiments with stitching and weaving, to the designing and making of bags, hats, and costumes that children can use--is the focus of this curriculum guide. For each grade (2-6), guidelines are provided on (1)…

  11. Cloning and characterization of Rep-8 (D8S2298E) in the human chromosome 8p11.2-p12

    SciTech Connect

    Yamabe, Yukako; Ichikawa, Koji; Sugawara, Kahori

    1997-01-15

    A novel human gene referred to as the Rep-8 gene (D8S2298E) was cloned by a combination of exon trapping, thermal asymmetric interlaced-PCR, and screening of a cDNA library. It is located in human chromosome 8p.11.2-p12. The gene consists of eight exons and spans about 20 kb between the glutathione S-reductase and the protein phosphatase 2A beta subunit genes. The full-length Rep-8 gene contains 1483 nucleotides and codes for a protein of 270 amino acids. Southern blot experiments showed that the Rep-8 gene exists as a single copy per haploid. With a zoo blot analysis, human Rep-8 DNA hybridized strongly with the monkey DNA, but only weakly with the DNAs of species other than Homo sapiens. Northern blot analysis showed that it is expressed abundantly in the testis and ovary, suggesting that the Rep-8 gene product may play a role in reproduction. 16 refs., 5 figs., 1 tab.

  12. 76 FR 5066 - Airworthiness Directives; Pratt & Whitney JT8D-7, -7A, -7B, -9, -9A, -11, -15, -15A, -17, -17A...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-28

    ...-14398 (70 FR 73361, December 12, 2005). That AD applies to the specified products. The NPRM published in the Federal Register on August 18, 2010 (75 FR 50942). That NPRM proposed to modify the TLS of the... ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979), (3) Will...

  13. 75 FR 50942 - Airworthiness Directives; Pratt & Whitney JT8D-7, -7A, -7B, -9, -9A, -11, -15, -15A, -17, -17A...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ..., 2000 (65 FR 19477-78). Examining the AD Docket You may examine the AD docket on the Internet at http... AD 2005-25-05, Amendment 39- 14398 (70 FR 73361, December 12, 2005), to require revisions to the TLS.... Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034,...

  14. 75 FR 62319 - Airworthiness Directives; Pratt & Whitney JT8D-9, -9A, -11, -15, -17, and -17R Turbofan Engines

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-08

    ..., Engine Certification Office, FAA, Engine & Propeller Directorate, 12 New England Executive Park... Federal Register on May 19, 2010 (75 FR 27972). That action proposed to require overhauling fan blade... ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and (3)...

  15. Thermally Tunable Dual Emission of the d(8)-d(8) Dimer [Pt2(μ-P2O5(BF2)2)4](4).

    PubMed

    Hofbeck, Thomas; Lam, Yan Choi; Kalbáč, Martin; Záliš, Stanislav; Vlček, Antonín; Yersin, Hartmut

    2016-03-01

    High-resolution fluorescence, phosphorescence, as well as related excitation spectra, and, in particular, the emission decay behavior of solid [Bu4N]4[Pt2(μ-P2O5(BF2)2)4], abbreviated Pt(pop-BF2), have been investigated over a wide temperature range, 1.3-310 K. We focus on the lowest excited states that result from dσ*pσ (5dz(2)-6pz) excitations, i.e., the singlet state S1 (of (1)A2u symmetry in D4h) and the lowest triplet T1, which splits into spin-orbit substates A1u((3)A2u) and Eu((3)A2u). After optical excitation, an unusually slow intersystem crossing (ISC) is observed. As a consequence, the compound shows efficient dual emission, consisting of blue fluorescence and green phosphorescence with an overall emission quantum yield of ∼ 100% over the investigated temperature range. Our investigation sheds light on this extraordinary dual emission behavior, which is unique for a heavy-atom transition metal compound. Direct ISC processes in Pt(pop-BF2) are largely forbidden due to spin-, symmetry-, and Franck-Condon overlap-restrictions and, therefore, the ISC time is as long as 29 ns for T < 100 K. With temperature increase, two different thermally activated pathways, albeit still relatively slow, are promoted by spin-vibronic and vibronic mechanisms, respectively. Thus, distinct temperature dependence of the ISC processes results and, as a consequence, also of the fluorescence/phosphorescence intensity ratio. The phosphorescence lifetime also is temperature-dependent, reflecting the relative population of the triplet T1 substates Eu and A1u. The highly resolved phosphorescence shows a ∼ 220 cm(-1) red shift below 10 K, attributable to zero-field splitting of 40 cm(-1) plus a promoting vibration of 180 cm(-1). PMID:26909653

  16. 30 CFR 901.15 - Approval of Alabama regulatory program amendments.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...-X-: 2A-.06(xx), (yy), (fff), (www)(5), (kkkkkk)(1); 5A-.02(1)(i), .17(1)(n), .18, .36; 8C-.09; 8D... 5, 1984 ASMC Rules 880-X-8C-.06; 10C-.13, .17, .20, .27, .36; 10D-.13, .17, .20, .25, .33; Ala. revised systems Ch. V, § 731.14(f), (g)(9). January 9, 1984 September 27, 1984 ASMC Rules...

  17. Formation of high-molecular-weight compounds via the heterogeneous reactions of gaseous C8-C10 n-aldehydes in the presence of atmospheric aerosol components

    NASA Astrophysics Data System (ADS)

    Han, Yuemei; Kawamura, Kimitaka; Chen, Qingcai; Mochida, Michihiro

    2016-02-01

    A laboratory study on the heterogeneous reactions of straight-chain aldehydes was performed by exposing n-octanal, nonanal, and decanal vapors to ambient aerosol particles. The aerosol and blank filters were extracted using methanol. The extracts were nebulized and the resulting compositions were examined using a high-resolution time-of-flight aerosol mass spectrometer. The mass spectral analysis showed that the exposures of the aldehydes to aerosol samples increased the peak intensities in the high mass range. The peaks in the mass spectra of the aerosol samples after exposure to different aldehydes were characterized by a homologous series of peak shifts due to the addition of multiple CH2 units. This result is explained by the formation of high-molecular-weight (HMW) compounds that contain single or multiple aldehyde moieties. The HMW fragment peaks for the blank filters exposed to n-aldehydes were relatively weak, indicating an important contribution from the ambient aerosol components to the formation of the HMW compounds. Among the factors affecting the overall interaction of aldehydes with atmospheric aerosol components, gas phase diffusion possibly limited the reactions under the studied conditions; therefore, their occurrence to a similar degree in the atmosphere is not ruled out, at least for the reactions involving n-nonanal and decanal. The major formation pathways for the observed HMW products may be the self-reactions of n-aldehydes mediated by atmospheric aerosol components and the reactions of n-aldehydes with organic aerosol components. The observed formation of HMW compounds encourages further investigations into their effects on the aerosol properties as well as the organic aerosol mass in the atmosphere.

  18. 75 FR 50891 - N-alkyl (C8-C18) Primary Amines and Acetate Salts; Exemption from the Requirement of a Tolerance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... the Federal Register of February 4, 2010, (75 FR 5793) (FRL- 8807-5), EPA issued a notice pursuant to..., (74 FR 37578) (FRL-8428-9). C. Exposure Assessment 1. Dietary exposure from food and feed uses. In... types of actions from review under Executive Order 12866, entitled Regulatory Planning and Review (58...

  19. First-principles study on Ca 8C n ( n⩽12) and Ca mC 12 ( m⩽8) metal carbides

    NASA Astrophysics Data System (ADS)

    Chen, Gang; Peng, Qi; Kawazoe, Yoshiyuki

    2011-02-01

    We performed an extensive structure optimization for small Ca8Cn (n⩽12) and CamC12 (m⩽8) clusters by using density functional theory. The structure would gain CaC2 bulk characters for Ca8Cn with n⩾7 and CamC12 with m⩾6, respectively. Two cage-like structures were found and their structural stability was evaluated by molecular dynamics simulations. The Ca 4s valence electrons are donated to the π orbitals of dicarbon and part of the donated electrons is back donated to the Ca 3d split orbitals. Both Ca and C atoms contribute to the magnetic properties. The net charge on Ca analyzed with Bader technique is about +1.4e.

  20. Fission barriers for the emission of odd-numbered fragments from multicharged C{sub 60} prepared in Ar{sup 8+}-C{sub 60} collisions

    SciTech Connect

    Martin, S.; Chen, L.; Bernard, J.; Buchet-Poulizac, M. C.; Wei, B.; Bredy, R.

    2006-01-15

    We report on measurements of the branching ratios of emission of small C{sub n}{sup +} fragments in asymmetrical fission of highly charged C{sub 60}{sup r+} ions (r=4-6). For the channels corresponding to the emission of one fragment, only small fragments with an even number of carbon atoms are observed. For the channels with the emission of two fragments, successive emission of small fragments with an odd number of carbon atoms has been observed with a surprisingly high branching ratio (30%). In order to reproduce the experimental branching ratios in the framework of a statistical evaporation model, the height of fission barriers for the emission of one odd numbered fragment has to be reduced at higher temperature in order to allow the opening of these channels that are forbidden at lower temperature.

  1. Effects of flaps on buffet characteristics and wind-rock onset of an F-8C airplane at subsonic and transonic speeds

    NASA Technical Reports Server (NTRS)

    Monaghan, R. C.; Friend, E. L.

    1973-01-01

    Wind-up-turn maneuvers were performed to establish the values of airplane normal force coefficient for buffet onset, wing-rock onset, and buffet loads with various combinations of leading- and trailing-edge flap deflections. Data were gathered at both subsonic and transonic speeds covering a range from Mach 0.64 to Mach 0.92. Buffet onset and buffet loads were obtained from wingtip acceleration and wing-root bending-moment data, and wing-rock onset was obtained from airplane roll rate data. Buffet onset, wing-rock onset, and buffet loads were similarly affected by the various combinations of leading- and training-edge flaps. Subsonically, the 12 deg leading-edge-flap and trailing-edge-flap combination was most effective in delaying buffet onset, wing-rock onset, and equivalent values of buffet loads to a higher value of airplane normal force coefficient. This was the maximum flap deflection investigated. Transonically, however, the optimum leading-edge flap position was generally less than 12 deg.

  2. The Fas-associated death domain protein/caspase-8/c-FLIP signaling pathway is involved in TNF-induced activation of ERK

    SciTech Connect

    Lueschen, Silke; Falk, Markus; Scherer, Gudrun; Ussat, Sandra; Paulsen, Maren; Adam-Klages, Sabine . E-mail: sadam@email.uni-kiel.de

    2005-10-15

    The cytokine TNF activates multiple signaling pathways leading to cellular responses ranging from proliferation and survival to apoptosis. While most of these pathways have been elucidated in detail over the past few years, the molecular mechanism leading to the activation of the MAP kinases ERK remains ill defined and is controversially discussed. Therefore, we have analyzed TNF-induced ERK activation in various human and murine cell lines and show that it occurs in a cell-type-specific manner. In addition, we provide evidence for the involvement of the signaling components Fas-associated death domain protein (FADD), caspase-8, and c-FLIP in the pathway activating ERK in response to TNF. This conclusion is based on the following observations: (I) Overexpression of FADD, caspase-8, or a c-FLIP protein containing the death effector domains only leads to enhanced and prolonged ERK activation after TNF treatment. (II) TNF-induced ERK activation is strongly diminished in the absence of FADD. Interestingly, the enzymatic function of caspase-8 is not required for TNF-induced ERK activation. Additional evidence suggests a role for this pathway in the proliferative response of murine fibroblasts to TNF.

  3. Ground wave emergency network final operational capability. Environmental assessment for southern Arkansas relay node site no. RN 8C912AR

    NASA Astrophysics Data System (ADS)

    1993-02-01

    The Ground Wave Emergency Network (GWEN) is a radio communication system designed to relay emergency messages between strategic military areas in the continental United States. The system is immune to the effects of high-attitude electromagnetic pulse (HEMP) energy surges caused by nuclear bursts in the ionosphere that would disrupt conventional communications equipment such as telephones and shortwave radios. A failure of such equipment would prevent timely communications among top military and civilian leaders and strategic Air Force locations and prevent U.S. assessment and retaliation during an attack. GWEN is an essential part of a defense modernization program to upgrade and improve our nation's communications system, thereby strengthening deterrence.

  4. Ground wave emergency network final operational capability: Environmental assessment for northwestern Nebraska relay node, site number RN 8C930NE

    NASA Astrophysics Data System (ADS)

    1993-02-01

    The Ground Wave Emergency Network (GWEN) is a radio communication system designed to relay emergency messages between strategic military areas in the continental United States. The system is immune to the effects of high-altitude electromagnetic pulse (HEMP) energy surges caused by nuclear bursts in the ionosphere that would disrupt conventional communications equipment such as telephones and shortwave radios. A failure of such equipment would prevent timely communications among top military and civilian leaders and strategic Air Force locations and prevent U.S. assessment and retaliation during an attack. GWEN is an essential part of a defense modernization program to upgrade and improve our nation's communications system, thereby strengthening deterrence. The GWEN system consists of a network of relay nodes, receive-only stations, and input/output stations. Each relay node, such as the one proposed in northwestern Nebraska, consists of a guyed radio tower facility similar to those used by commercial AM broadcast transmitters.

  5. Regioselectivity of Sc2C2@C3v(8)-C82: Role of the Sumanene-Type Hexagon in Diels-Alder Reaction.

    PubMed

    Zhao, Pei; Zhao, Xiang; Ehara, Masahiro

    2016-09-16

    Recently, several experiments have demonstrated high chemical reactivity of the sumanene-type hexagon in Sc2C2@C82. To further uncover its reactivity, the Diels-Alder reaction to all the nonequivalent C-C bonds of C82 and Sc2C2@C82 has been investigated by density functional theory calculations. For the free fullerene, the [5,6] bond 7 is the thermodynamically most favored, whereas the addition on the [6,6] bond 3 has the lowest activation energy. Diels-Alder reaction has no preference for addition sites in the sumanene-type hexagon. However, in the case of the endohedral fullerene, the [6,6] bond 19 in the special hexagon becomes the most reactive site according to both kinetic and thermodynamic considerations. Further analyses reveal that bond 19 in Sc2C2@C82 exhibits the shortest bond length and third largest π-orbital axis vector. In addition, the LUMOs of bond 19 are also symmetry-allowed to interact with butadiene. PMID:27538115

  6. Association of HSL gene E1-c.276C>T and E8-c.51C>T mutation with economical traits of Chinese Simmental cattle.

    PubMed

    Fang, X B; Zhang, L P; Yu, X Z; Li, J Y; Lu, C Y; Zhao, Z H; Yang, R J

    2014-01-01

    Hormone-sensitive lipase (HSL) is responsible for the decomposition of triglycerides in adipose tissue to release free fatty acids, and it is a key rate-limiting enzyme in the regulation of adipose tissue deposition and decomposition. The objective of this study was to evaluate the association between novel SNPs in the coding region of bovine HSL gene and carcass and meat quality traits of Chinese Simmental-cross steers. Two novel SNPs were genotyped and the 47 traits of carcass and meat quality traits were measured in the population studied. Statistical analysis revealed that the SNPs of HSL gene were associated with the carcass and meat quality traits. The individuals with TT genotypes of E1-276C>T showed significant higher dressing percentage, net meat rate, hind legs circumference, fat coverage rate, mesenteric fat and kidney fat (p < 0.05). E8-51C>T (P17S) also showed a significant association with the pH of beef and fatty acids content in Chinese Simmental cattle (p < 0.01). Our findings indicated that polymorphisms in HSL might be one of important genetic factors that influence carcass yield and meat quality in beef cattle, and it may be a useful marker for meat quality traits in future marker-assisted selection programs in beef cattle breeding and production. PMID:24213829

  7. Longitudinal aerodynamic characteristics of a wing-winglet model designed at M = 0.8, C sub L = 0.4 using linear aerodynamic theory

    NASA Technical Reports Server (NTRS)

    Kuhlman, J. M.

    1983-01-01

    Wind tunnel test results have been presented herein for a subsonic transport type wing fitted with winglets. Wind planform was chosen to be representative of wings used on current jet transport aircraft, while wing and winglet camber surfaces were designed using two different linear aerodynamic design methods. The purpose of the wind tunnel investigation was to determine the effectiveness of these linear aerodynamic design computer codes in designing a non-planar transport configuration which would cruise efficiently. The design lift coefficient was chosen to be 0.4, at a design Mach number of 0.8. Force and limited pressure data were obtained for the basic wing, and for the wing fitted with the two different winglet designs, at Mach numbers of 0.60, 0.70, 0.75 and 0.80 over an angle of attack range of -2 to +6 degrees, at zero sideslip. The data have been presented without analysis to expedite publication.

  8. Association of HSL gene E1-c.276C>T and E8-c.51C>T mutation with economical traits of Chinese Simmental cattle.

    PubMed

    Fang, X B; Zhang, L P; Yu, X Z; Li, J Y; Lu, C Y; Zhao, Z H; Yang, R J

    2014-01-01

    Hormone-sensitive lipase (HSL) is responsible for the decomposition of triglycerides in adipose tissue to release free fatty acids, and it is a key rate-limiting enzyme in the regulation of adipose tissue deposition and decomposition. The objective of this study was to evaluate the association between novel SNPs in the coding region of bovine HSL gene and carcass and meat quality traits of Chinese Simmental-cross steers. Two novel SNPs were genotyped and the 47 traits of carcass and meat quality traits were measured in the population studied. Statistical analysis revealed that the SNPs of HSL gene were associated with the carcass and meat quality traits. The individuals with TT genotypes of E1-276C>T showed significant higher dressing percentage, net meat rate, hind legs circumference, fat coverage rate, mesenteric fat and kidney fat (p < 0.05). E8-51C>T (P17S) also showed a significant association with the pH of beef and fatty acids content in Chinese Simmental cattle (p < 0.01). Our findings indicated that polymorphisms in HSL might be one of important genetic factors that influence carcass yield and meat quality in beef cattle, and it may be a useful marker for meat quality traits in future marker-assisted selection programs in beef cattle breeding and production.

  9. N-[3a-(4-Bromo-phen-yl)-8b-hy-droxy-6,8-dimeth-oxy-3-phenyl-2,3,3a,8b-tetra-hydro-1H-cyclo-penta-[b]benzofuran-1-yl]formamide monohydrate.

    PubMed

    Aubert, Emmanuel; Thuaud, Frédéric; Ribeiro, Nigel; Désaubry, Laurent; Espinosa, Enrique

    2013-01-01

    In the title compound, C26H24BrNO5·H2O, a synthetic analogue of natural flavagline, the cyclo-pentane ring adopts an envelope conformation (the flap atom bearing the phenyl group) and the vicinal phenyl and bromo-phenyl groups are slightly shifted relative to each other [CPh-C-C-CPhBr = 36.3 (2)°]. Intra-molecular N-H⋯O and C-H⋯O hydrogen bonds form S(5) motifs. In the crystal, the organic and the water mol-ecules are linked by an O-H⋯O hydrogen bond. Pairs of organic and water mol-ecules, located about inversion centers, inter-act through O-H⋯O hydrogen bonds, forming R4(4)(20) and R4(4)(26) motifs, which together lead to C2(2)(9) motifs. The crystal packing is also characterized by N-H⋯O and C-H⋯O hydrogen bonds between neighbouring organic mol-ecules, forming R2(2)(10) and R2(2)(18) motifs, respectively. PMID:23476436

  10. 75 FR 14128 - Center for Nanoscale Science and Technology Postdoctoral Researcher and Visiting Fellow...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-24

    ... February 11, 2008 (73 FR 7696), are applicable to this notice on the form SF-424 items 8.b. and 8.c., the... Department of Commerce Pre-Award Notification Requirements, 73 FR 7696 (Feb. 11, 2008). Questions about these...,505 (74 FR 10667, March 9, 2009), revoking previous Executive Orders and Presidential...

  11. 12 CFR Appendix A to Part 363 - Guidelines and Interpretations

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Control 11. Service Organizations 12. Reserved Role of Independent Public Accountant (§ 363.3) 13. General.... Management's Reports on Internal Control over Financial Reporting under Part 363 and Section 404 of SOX 8B. Internal Control Reports and Part 363 Annual Reports for Acquired Businesses 8C. Management's Disclosure...

  12. 12 CFR Appendix A to Part 363 - Guidelines and Interpretations

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Control 11. Service Organizations 12. Reserved Role of Independent Public Accountant (§ 363.3) 13. General.... Management's Reports on Internal Control over Financial Reporting under Part 363 and Section 404 of SOX 8B. Internal Control Reports and Part 363 Annual Reports for Acquired Businesses 8C. Management's Disclosure...

  13. 12 CFR Appendix A to Part 363 - Guidelines and Interpretations

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Control 11. Service Organizations 12. Reserved Role of Independent Public Accountant (§ 363.3) 13. General.... Management's Reports on Internal Control over Financial Reporting under Part 363 and Section 404 of SOX 8B. Internal Control Reports and Part 363 Annual Reports for Acquired Businesses 8C. Management's Disclosure...

  14. Isolation and functional characterisation of the genes encoding Δ(8)-sphingolipid desaturase from Brassica rapa.

    PubMed

    Li, Shu-Fen; Song, Li-Ying; Yin, Wei-Bo; Chen, Yu-Hong; Chen, Liang; Li, Ji-Lin; Wang, Richard R-C; Hu, Zan-Min

    2012-01-01

    Δ(8)-Sphingolipid desaturase is the key enzyme that catalyses desaturation at the C8 position of the long-chain base of sphingolipids in higher plants. There have been no previous studies on the genes encoding Δ(8)-sphingolipid desaturases in Brassica rapa. In this study, four genes encoding Δ(8)-sphingolipid desaturases from B. rapa were isolated and characterised. Phylogenetic analyses indicated that these genes could be divided into two groups: BrD8A, BrD8C and BrD8D in group I, and BrD8B in group II. The two groups of genes diverged before the separation of Arabidopsis and Brassica. Though the four genes shared a high sequence similarity, and their coding desaturases all located in endoplasmic reticulum, they exhibited distinct expression patterns. Heterologous expression in Saccharomyces cerevisiae revealed that BrD8A/B/C/D were functionally diverse Δ(8)-sphingolipid desaturases that catalyse different ratios of the two products 8(Z)- and 8(E)-C18-phytosphingenine. The aluminium tolerance of transgenic yeasts expressing BrD8A/B/C/D was enhanced compared with that of control cells. Expression of BrD8A in Arabidopsis changed the ratio of 8(Z):8(E)-C18-phytosphingenine in transgenic plants. The information reported here provides new insights into the biochemical functional diversity and evolutionary relationship of Δ(8)-sphingolipid desaturase in plants and lays a foundation for further investigation of the mechanism of 8(Z)- and 8(E)-C18-phytosphingenine biosynthesis.

  15. 40 CFR Table 10 to Subpart Hhhhh... - Applicability of General Provisions to Subpart HHHHH

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 CFR part 63. This subpart does not contain requirements for continuous opacity monitoring systems... SS of 40 CFR part 63. § 63.8(c)(7)-(8) CMS Requirements Only for CEMS. Requirements for CPMS are specified in referenced subpart SS of 40 CFR part 63. § 63.8(d) CMS Quality Control Only for...

  16. 40 CFR Table 10 to Subpart Hhhhh... - Applicability of General Provisions to Subpart HHHHH

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 CFR part 63. This subpart does not contain requirements for continuous opacity monitoring systems... SS of 40 CFR part 63. § 63.8(c)(7)-(8) CMS Requirements Only for CEMS. Requirements for CPMS are specified in referenced subpart SS of 40 CFR part 63. § 63.8(d) CMS Quality Control Only for...

  17. 40 CFR Table 10 to Subpart Hhhhh... - Applicability of General Provisions to Subpart HHHHH

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 CFR part 63. This subpart does not contain requirements for continuous opacity monitoring systems... SS of 40 CFR part 63. § 63.8(c)(7)-(8) CMS Requirements Only for CEMS. Requirements for CPMS are specified in referenced subpart SS of 40 CFR part 63. § 63.8(d) CMS Quality Control Only for...

  18. 40 CFR Table 10 to Subpart Hhhhh... - Applicability of General Provisions to Subpart HHHHH

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 CFR part 63. This subpart does not contain requirements for continuous opacity monitoring systems... SS of 40 CFR part 63. § 63.8(c)(7)-(8) CMS Requirements Only for CEMS. Requirements for CPMS are specified in referenced subpart SS of 40 CFR part 63. § 63.8(d) CMS Quality Control Only for...

  19. 75 FR 40829 - Agency Information Collection Activities: Existing Collection; Emergency Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-14

    .... Abstract: Section 709(c) of Title VII of the Civil Rights Act of 1964, as amended, 42 U.S.C. 2000e-8(c... Title VII of the Civil Rights Act of 1964, U.S.C. 2000e-8(d), as amended, EEO-4 data is shared with... Employment Opportunity Commission. ACTION: Notice of information collection--emergency......

  20. 76 FR 4347 - Agency Information Collection Activities: Existing Collection; Emergency Extension

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-25

    .... Abstract: Section 709(c) of Title VII of the Civil Rights Act of 1964, as amended, 42 U.S.C. 2000e-8(c... Title VII of the Civil Rights Act of 1964, U.S.C. 2000e-8(d), as amended, EEO-4 data is shared with... Employment Opportunity Commission. ACTION: Notice of Information Collection--Emergency......

  1. 75 FR 56483 - Fisheries of the Exclusive Economic Zone Off Alaska; Reallocation of Crab and Halibut Prohibited...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-16

    ... established by the final 2010 and 2011 harvest specifications for groundfish in the BSAI (75 FR 11778, March... the BSAI (75 FR 11778, March 12, 2010) are revised as follows in Tables 8a, 8c, and 8d: Table 8a... Economic Zone Off Alaska; Reallocation of Crab and Halibut Prohibited Species Catch Allowances in...

  2. 40 CFR Table 10 to Subpart Hhhhh... - Applicability of General Provisions to Subpart HHHHH

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 CFR part 63. This subpart does not contain requirements for continuous opacity monitoring systems... SS of 40 CFR part 63. § 63.8(c)(7)-(8) CMS Requirements Only for CEMS. Requirements for CPMS are specified in referenced subpart SS of 40 CFR part 63. § 63.8(d) CMS Quality Control Only for...

  3. 40 CFR Appendix A to Subpart Ll of... - Applicability of General Provisions

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requirements under § 63.845. 63.7(a)(2)(ii) and (iii) Performance testing requirements No Subpart LL specifies performance test dates. 63.8(c)(4)-(c)(8) CMS operation and maintenance No Subpart LL does not require COMS/CMS or CMS performance specifications. 63.8(d) Quality control No Subpart LL does not require CMS...

  4. Revisiting the chemistry of the actinocenes [(η8-C8H8)2An] (An = U, Th) with neutral Lewis bases. Access to the bent sandwich complexes [(η8-C8H8)2An(L)] with thorium (L = py, 4,4'-bipy, tBuNC, R4phen).

    PubMed

    Berthet, Jean-Claude; Thuéry, Pierre; Garin, Nicolas; Dognon, Jean-Pierre; Cantat, Thibault; Ephritikhine, Michel

    2013-07-10

    In stark contrast to uranocene, (Cot)2Th reacts with neutral mono- or bidentate Lewis bases to give the bent sandwich complexes (Cot)2Th(L) (L = py, 4,4'-bipy, tBuNC, phen, Me4phen). DFT calculations in the gas phase show that, for both U and Th, formation of the bent compound (Cot)2An(L) should be facile, the linear and bent forms being close in energy.

  5. PROCEEDINGS: 1991 INTERNATIONAL CONFERENCE ON MUNICIPAL WASTE COMBUSTION - VOLUME 2. SESSIONS 1B, 2B, 3B, 4B, 7A, 7B, 8A, 8B, AND 9B

    EPA Science Inventory

    The three-volumes document 82 presentations by authors from 15 countries at the Second International Conference on Municipal Waste Combustion (MWC) in Tampa, Florida, April 16-19, 1991. The Conference fostered the exchange of current information on research concerning MWC, ash di...

  6. The Attorney General's Asbestos Liability Report to the Congress. Pursuant to Section 8(b) of the Asbestos School Hazard Detection and Control Act of 1980. Committee Print, Ninety-Seventh Congress, First Session.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and Labor.

    The Attorney General was directed by Congress to prepare a report on whether the United States could recover, from any persons determined liable, the amounts expended to detect, contain, or remove hazardous asbestos products from schools. The general background portion of this report contains the results of the factual research and investigation.…

  7. Synthesis of unsymmetrical monocarbonyl curcumin analogues with potent inhibition on prostaglandin E2 production in LPS-induced murine and human macrophages cell lines.

    PubMed

    Mohd Aluwi, Mohd Fadhlizil Fasihi; Rullah, Kamal; Yamin, Bohari M; Leong, Sze Wei; Abdul Bahari, Mohd Nazri; Lim, Sock Jin; Mohd Faudzi, Siti Munirah; Jalil, Juriyati; Abas, Faridah; Mohd Fauzi, Norsyahida; Ismail, Nor Hadiani; Jantan, Ibrahim; Lam, Kok Wai

    2016-05-15

    The syntheses and bioactivities of symmetrical curcumin and its analogues have been the subject of interest by many medicinal chemists and pharmacologists over the years. To improve our understanding, we have synthesized a series of unsymmetrical monocarbonyl curcumin analogues and evaluated their effects on prostaglandin E2 production in lipopolysaccharide-induced RAW264.7 and U937 cells. Initially, compounds 8b and 8c exhibited strong inhibition on the production of PGE2 in both LPS-stimulated RAW264.7 (8b, IC50=12.01μM and 8c, IC50=4.86μM) and U937 (8b, IC50=3.44μM and 8c, IC50=1.65μM) cells. Placing vanillin at position Ar2 further improved the potency when both compounds 15a and 15b significantly lowered the PGE2 secretion level (RAW264.7: 15a, IC50=0.78μM and 15b, IC50=1.9μM while U937: 15a, IC50=0.95μM and 15b, IC50=0.92μM). Further experiment showed that compounds 8b, 8c, 15a and 15b did not target the activity of downstream inflammatory COX-2 mediator. Finally, docking simulation on protein targets COX-2, IKK-β, ERK, JNK2, p38α and p38β were performed using the conformation of 15a determined by single-crystal XRD. PMID:27040659

  8. Program on ground test of modified quiet, clean, JT3D and JT8D turbofan engines in their respective nacelles. [modification of Boeing 707, 727, and 737 aircraft for aircraft noise reduction

    NASA Technical Reports Server (NTRS)

    1973-01-01

    A program to reduce the community noise levels of commercial jet aircraft is summarized. The program objective is the development of three acoustically treated nacelle configurations for the 707, 727, and 737 series aircraft to provide maximum noise reduction with minimum performance loss, modification requirements, and economic impact. The preliminary design, model testing, data analyses, and economic studies of proposed nacelle configurations are discussed.

  9. Evaluation of craniofacial surgery in the treatment of facial deformities.

    PubMed Central

    Murray, J E; Swanson, L T; Strand, R D; Hricko, G M

    1975-01-01

    . Fig. 1g. Fig. 1h. Fig. 1i. Fig. 1m. Fig. 2a. Fig. 2b., Fig. 2c., Fig. 2d. Fig. 2e. Figs. 3 a and b. Fig. 3d., Fig. 3e. Fig. 3f. Fig. 4a., Fig. 4b. Fig. 5a. Fig. 5b., Fig. 5c. Figs. 5d and e. Fig. 5f. Figs. 5g. and 5h. Fig. 6a., Fig. 6b. Fig. 6c. Fig. 7a. Fig. 7b. Figs. 7e and f. Fig. 8a. Fig. 8b. Fig. 8c. Fig. 8d., Fig. 8e. Fig. 9a. Fig. 9b. Figs. 9c. and d. Fig. 9e. Fig. 9f. Figs. 9g. and h. Figs. 11a, b, and c. Figs. 11d, e, f. PMID:169753

  10. PROCEEDINGS: 1991 INTERNATIONAL CONFERENCE ON MUNICIPAL WASTE COMBUSTION - VOLUME 3. SESSIONS 1C, 2C, 3C 4C, 6C, 7C, 8C, 9A, AND 10A/C

    EPA Science Inventory

    The three-volumes document 82 presentations by authors from 15 countries at the Second International Conference on Municipal Waste Combustion (MWC) in Tampa, Florida, April 16-19, 1991. The Conference fostered the exchange of current information on research concerning MWC, ash di...

  11. Low temperature rate coefficients for reactions of the butadiynyl radical, C4H, with various hydrocarbons. Part I: reactions with alkanes (CH4, C2H6, C3H8, C4H10).

    PubMed

    Berteloite, Coralie; Le Picard, Sébastien D; Balucani, Nadia; Canosa, André; Sims, Ian R

    2010-04-21

    The kinetics of the reactions of the linear butadiynyl radical, C4H (CCCCH), with methane, ethane, propane and butane have been studied over the temperature range of 39-300 K using a CRESU (Cinétique de Réaction en Ecoulement Supersonique Uniforme or Reaction Kinetics in Uniform Supersonic Flow) apparatus combined with the pulsed laser photolysis-laser induced fluorescence technique. The rate coefficients, except for the reaction with methane, show a negative temperature dependence and can be fitted with the following expressions over the temperature range of this study: k(C2H6) = 0.289 x 10(-10) (T/298 K)(-1.23) exp(-24.8 K/T) cm3 molecule(-1) s(-1); k(C3H8) = 1.06 x 10(-10) (T/298 K)(-1.36) exp(-56.9 K/T) cm3 molecule(-1) s(-1); k(C4H10) = 2.93 x 10(-10) (T/298 K)(-1.30) exp(-90.1 K/T) cm3 molecule(-1) s(-1). The rate coefficients for the reaction with methane were measured only at 200 K and 300 K yielding a positive temperature dependence: k(CH4) = 1.63 x 10(-11) exp(-610 K/T) cm3 molecule(-1) s(-1). Possible reaction mechanisms and product channels are discussed in detail for each of these reactions. Potential implications of these results for models of low temperature chemical environments, in particular cold interstellar clouds and planetary atmospheres such as that of Titan, are considered.

  12. Novel third-generation water-soluble noscapine analogs as superior microtubule-interfering agents with enhanced antiproliferative activity

    PubMed Central

    Henary, Maged; Narayana, Lakshminarayana; Ahad, Shazia; Gundala, Sushma R.; Mukkavilli, Rao; Sharma, Vibhuti; Owens, Eric A.; Yadav, Yogesh; Nagaraju, Mulpuri; Hamelberg, Donald; Tandon, Vibha; Panda, Dulal; Aneja, Ritu

    2015-01-01

    Noscapine, an opium-derived ‘kinder-gentler’ microtubule-modulating drug is in Phase I/II clinical trials for cancer chemotherapy. However, its limited water solubility encumbers its development into an oral anticancer drug with clinical promise. Here we report the synthesis of 9 third-generation, water-soluble noscapine analogs with negatively charged sulfonato and positively charged quaternary ammonium groups using noscapine, 9-bromonoscapine and 9-aminonoscapine as scaffolds. The predictive free energy of solvation was found to be lower for sulfonates (6a–c;8a–c) compared to the quaternary ammonium-substituted counterparts, explaining their higher water solubility. In addition, sulfonates showed higher charge dispersability, which may effectively shield the hydrophobicity of isoquinoline nucleus as indicated by hydrophobicity mapping methods. These in silico data underscore efficient net charge balancing, which may explain higher water solubility and thus enhanced antiproliferative efficacy and improved bioavailability. We observed that 6b, 8b and 8c strongly inhibited tubulin polymerization and demonstrated significant antiproliferative activity against four cancer cell lines compared to noscapine. Molecular simulation and docking studies of tubulin-drug complexes revealed that the brominated compound with a four-carbon chain (4b, 6b, 8b) showed optimal binding with tubulin heterodimers. Interestingly, 6b, 8b and 8c treated PC-3 cells resulted in preponderance of mitotic cells with multipolar spindle morphology, suggesting that they stall the cell cycle. Furthermore, in vivo pharmacokinetic evaluation of 6b, 8b and 8c revealed at least 1–2 fold improvement in their bioavailability compared to noscapine. To our knowledge, this is the first report to demonstrate novel water-soluble noscapine analogs that may pave the way for future pre-clinical drug development. PMID:25124704

  13. Novel third-generation water-soluble noscapine analogs as superior microtubule-interfering agents with enhanced antiproliferative activity.

    PubMed

    Henary, Maged; Narayana, Lakshminarayana; Ahad, Shazia; Gundala, Sushma R; Mukkavilli, Rao; Sharma, Vibhuti; Owens, Eric A; Yadav, Yogesh; Nagaraju, Mulpuri; Hamelberg, Donald; Tandon, Vibha; Panda, Dulal; Aneja, Ritu

    2014-11-15

    Noscapine, an opium-derived 'kinder-gentler' microtubule-modulating drug is in Phase I/II clinical trials for cancer chemotherapy. However, its limited water solubility encumbers its development into an oral anticancer drug with clinical promise. Here we report the synthesis of 9 third-generation, water-soluble noscapine analogs with negatively charged sulfonato and positively charged quaternary ammonium groups using noscapine, 9-bromonoscapine and 9-aminonoscapine as scaffolds. The predictive free energy of solvation was found to be lower for sulfonates (6a-c; 8a-c) compared to the quaternary ammonium-substituted counterparts, explaining their higher water solubility. In addition, sulfonates showed higher charge dispersability, which may effectively shield the hydrophobicity of isoquinoline nucleus as indicated by hydrophobicity mapping methods. These in silico data underscore efficient net charge balancing, which may explain higher water solubility and thus enhanced antiproliferative efficacy and improved bioavailability. We observed that 6b, 8b and 8c strongly inhibited tubulin polymerization and demonstrated significant antiproliferative activity against four cancer cell lines compared to noscapine. Molecular simulation and docking studies of tubulin-drug complexes revealed that the brominated compound with a four-carbon chain (4b, 6b, and 8b) showed optimal binding with tubulin heterodimers. Interestingly, 6b, 8b and 8c treated PC-3 cells resulted in preponderance of mitotic cells with multipolar spindle morphology, suggesting that they stall the cell cycle. Furthermore, in vivo pharmacokinetic evaluation of 6b, 8b and 8c revealed at least 1-2-fold improvement in their bioavailability compared to noscapine. To our knowledge, this is the first report to demonstrate novel water-soluble noscapine analogs that may pave the way for future pre-clinical drug development. PMID:25124704

  14. Chemical constituents of Plectranthus amboinicus and the synthetic analogs possessing anti-inflammatory activity.

    PubMed

    Chen, Yuan-Siao; Yu, Hui-Ming; Shie, Jiun-Jie; Cheng, Ting-Jen Rachel; Wu, Chung-Yi; Fang, Jim-Min; Wong, Chi-Huey

    2014-03-01

    This study demonstrates that compounds 1-4 from an extract of Plectranthus amboinicus inhibit the binding of AP-1 to its consensus DNA sequence. Thymoquinone (5) was further identified as a nonpolar ingredient from the hexane extract of P. amboinicus to suppress the expression of lipopolysaccharide-induced tumor necrosis factor-alpha (TNF-α). We then synthesized 2-alkylidenyl-4-cyclopentene-1,3-diones as the designed biomimetics of thymoquinone, and found that compounds 8a, 8b and 8d were more potent TNF-α inhibitors. PMID:24491635

  15. Antifungal agents, Part 11. Biphenyl analogues of naftifine: synthesis and antifungal activities.

    PubMed

    Porretta, G C; Fioravanti, R; Biava, M; Artico, M; Villa, A; Simonetti, N

    1995-09-01

    A series of naftifine analogues having the biphenyl instead of the naphthyl moiety have been synthesized in a search devoted to study bioanalogues of clinically efficacious antifungal agents. The new derivatives were tested against Candida albicans by the direct contact method. They were also assayed against Gram-positive and Gram-negative bacteria and against some isolates of plant pathogenic fungi. Derivatives 8a, 8c, and 9a were found to be active against Candida albicans, derivative 5a was active against E. coli, a very resistant species to antimycotic agents, and derivatives 8a and 8b inhibited the plant pathogenic Rhizoctonia solani.

  16. Study of resonance interactions in polyatomic molecules on the basis of highly accurate experimental data: Set of strongly interacting Bands ν10(B1), ν7(B2), ν4(A2), ν8(B2), ν3(A1) and ν6(B1) of CH2=CD2

    NASA Astrophysics Data System (ADS)

    Ulenikov, O. N.; Gromova, O. V.; Bekhtereva, E. S.; Berezkin, K. B.; Kashirina, N. V.; Tan, T. L.; Sydow, C.; Maul, C.; Bauerecker, S.

    2016-09-01

    The highly accurate (experimental accuracy in line positions ~(1 - 3) ×10-4cm-1) FTIR ro-vibrational spectra of CH2=CD2 in the region of 600-1300 cm-1, where the fundamental bands ν10, ν7, ν4, ν8, ν3, and ν6 are located, were recorded and analyzed with the Hamiltonian model which takes into account resonance interactions between all six studied bands. About 12 200 ro-vibrational transitions belonging to these bands (that is considerably more than it was made in the preceding studies for the bands ν10, ν7, ν8, ν3 and ν6; transitions belonging to the ν4 band were assigned for the first time) were assigned in the experimental spectra with the maximum values of quantum numbers Jmax. / Kamax . equal to 31/20, 46/18, 33/11, 50/26, 44/20 and 42/21 for the bands ν10, ν7, ν4, ν8, ν3, and ν6, respectively. On that basis, a set of 133 vibrational, rotational, centrifugal distortion and resonance interaction parameters was obtained from the weighted fit. They reproduce values of 3920 initial "experimental" ro-vibrational energy levels (positions of about 12 200 experimentally recorded and assigned transitions) with the rms error drms = 2.3 ×10-4cm-1.

  17. Exxon Valdez oil spill: State/federal natural resource damage assessment final report. Effects of pink salmon (oncorhynchus gorbuscha) escapement level on egg retention, preemergent fry, and adult returns to the kodiak and chignik management areas caused by the Exxon Valdez oil spill. Fish/shellfish study numbers 7b and 8b. Final report

    SciTech Connect

    1993-12-01

    As a result of the 1989 Exxon Valdez oil spill, commercial salmon fishing in and around the Kodiak and Chignik areas was severely restricted throughout the 1989 season. Consequently, pink salmon escapements for these areas greatly exceeded targeted escapement objectives. Investigations were conducted within the Kodiak and Chignik Management Areas during 1989 and 1990 to determine if negative impacts on future odd-year brood line pink salmon production occurred as a result of overescapement in 1989.

  18. 1H NMR Study of the Enantioselective Binding of λ- and Δ-[Ru(bpy)2(m-bpy-GHK)]Cl2 to the Deoxynucleotide Duplex d(5'-C1G2C3G4A5A6T7T8C9G10C11G12-3')2

    PubMed Central

    Myari, A.; Garoufis, A.

    2005-01-01

    The interaction of the diastereomeric complexes Λ- and Δ - [Ru(bpy)2(m - GHK)]Cl2, (GHK = glycine-histidine-lysine) to the deoxynucleotide duplex d(5'-CGCGAATTCGCG-3')2 was studied by means of 1H NMR spectroscopy. The diastereomers interact with the oligonucleotide duplex differently. The Δ - [Ru(bpy)2 (m - GHK)]Cl2 is characterized by major groove binding close to the central part of the oligonucleotide, with both the peptide and the bipyridine ligand of the complex involved in the binding. The λ - [Ru(bpy)2 (m - bpy - GHK)]C2 binds loosely, approaching the helix from the minor groove. The NMR analysis shows that the peptide (GHK) binding has a determinative role in the interactions of both diastereomers with the oligonucleotide. PMID:18365093

  19. (2aR*,5S*,6aS*,8aS*,E)-Ethyl 5-hy­droxy-7,7,8a-trimethyl-8-oxo-2,2a,6,6a,7,8,8a,8b-octa­hydro-1H-penta­leno[1,6-bc]oxepine-4-carboxyl­ate

    PubMed Central

    Mehta, Goverdhan; Kumar, C. S. Ananda; Sen, Saikat

    2012-01-01

    The title compound, C17H24O5, featuring a 2-carbeth­oxy-3-oxepanone unit in its intra­molecularly O—H⋯O hydrogen-bonded enol form, was obtained via [(CF3CO2)2Rh]2-catal­ysed intra­molecular O—H bond insertion in the α-diazo-ω-hy­droxy-β-ketoester, ethyl 4-[(1S,3aS,6R,6aS)-6-hy­droxy-2,2,3a-trimethyl-3-oxo-octa­hydro­penta­len-1-yl]-2-diazo-3-oxobutano­ate. The seven-membered oxacyclic ring, thus constructed on a cis-fused diquinane platform, was found to adopt a distorted boat–sofa conformation. PMID:23476221

  20. Esthetic Dentistry

    PubMed Central

    McComb, D.

    1989-01-01

    The author of this article discusses the development of means to attach biologically acceptable polymers to tooth enamel, as well as the ongoing development of suitable reinforced plastics to provide esthetic improvement of patients' teeth. She describes a variety of esthetic dental problems and discusses their treatment. ImagesFigure 1aFigure 1bFigure 2aFigure 2bFigure 3aFigure 3bFigure 4aFigure 4bFigure 5aFigure 5bFigure 6Figure 7aFigure 7bFigure 8aFigure 8bFigure 8cFigure 9Figure 10 PMID:21248898

  1. Molecular design, synthesis and cell based HCV replicon assay of novel benzoxazole derivatives.

    PubMed

    Ismail, M A H; Adel, M; Ismail, N S M; Abouzid, K A M

    2013-03-01

    Hepatitis C virus inhibitors based on benzoxazole scaffold were designed based on molecular modeling simulation study including docking into the NS5B polymerase active site. Several compounds showed significant high simulation docking scores relative to the assigned benzimidazole lead compound. The designed compounds were synthesized, structurally elucidated and their antiviral activity was evaluated through cell-based replicon in cultured Huh 5-2 cells. A number of the synthesized compounds showed significant inhibitory activity ranging from (52.2% inhibition up to 98% at<50 µg/mL). N-Benzyl-2-phenylbenzo[1,3]oxazole-5-carboxamide (8b) and N-Phenethyl-2-phenylbenzo[1,3] oxazole-5-carboxamide (8c) demonstrated genuine HCV inhibitory activity with EC50 values of 41.6 and 24.5 µg/mL respectively.

  2. Zirconium and hafnium complexes containing N-alkyl-substituted amine biphenolate ligands: unexpected ligand degradation and divergent complex constitutions governed by N-alkyls.

    PubMed

    Liang, Lan-Chang; Chien, Chia-Cheng; Chen, Ming-Tsz; Lin, Sheng-Ta

    2013-07-01

    The reactivity and thermal stability of zirconium and hafnium complexes containing the N-alkyl-substituted amine biphenolate ligands of the type [RN(CH2-2-O-3,5-C6H2(tBu)2)2](2-) ([R-ONO](2-); R = tBu (1a), iPr (1b), or nPr (1c)) were investigated. The reactions of either [1a]M(OiPr)2 (M = Zr or Hf) with equimolar H2[1a] or M(OiPr)4(HOiPr) (M = Zr or Hf) with 2 equiv of H2[1a] at 25 °C in diethyl ether or 80 °C in toluene afford moderate yields of colorless crystals of M[1a](OiPr)(iPrOCH2-2-O-3,5-C6H2(tBu)2) (M = Zr (4a) or Hf (5a)). Controlled experiments revealed that the production of 4a and 5a proceeds via unexpected thermal degradation of H2[1a] that produces a highly reactive, transient ortho-quinone methide intermediate. Similar reactions employing H2[1b] and H2[1c], however, led to the formation of homoleptic bis-ligand complexes Zr[1b]2 (8b) and M[1c]2 (M = Zr (8c) or Hf (9c)) as colorless crystals. Decisive factors governing these divergent reaction pathways and complex constitutions are discussed. The X-ray structures of 4a, 5a, 8b, 8c, and 9c are presented.

  3. Survey of the terrestrial habitats and vegetation of Shetland, 1974 - a framework for long term ecological monitoring

    NASA Astrophysics Data System (ADS)

    Wood, C. M.; Bunce, R. G. H.

    2015-10-01

    A survey of the natural environment was undertaken in Shetland in 1974, after concern was expressed that large scale development from the new oil industry could threaten the natural features of the islands. A framework was constructed by the Institute of Terrestrial Ecology on which to select samples for the survey. The vegetation and habitat data that were collected, along with the sampling framework, have recently been made public via the following DOIs: doi:8c-cc4a-4ea8-b4be-f8bd7ee25342" target="_blank">10.5285/06fc0b8c-cc4a-4ea8-b4be-f8bd7ee25342 (Terrestrial habitat, vegetation and soil data from Shetland, 1974) and doi:10.5285/f1b3179e-b446-473d-a5fb-4166668da146 (Land Classification of Shetland 1974). In addition to providing valuable information about the state of the natural environment of Shetland, the repeatable and statistically robust methods developed in the survey were used to underpin the Countryside Survey, Great Britain's national long-term integrated environmental monitoring programme. The demonstration of the effectiveness of the methodology indicates that a repeat of the survey would yield statistics about ecological changes in the islands, such as those arising from the impacts of the oil industry. Currently no such figures are available although there is much information on the sociological impacts, as well as changes in agriculture.

  4. Leucine-rich repeat containing protein LRRC8A is essential for swelling-activated Cl- currents and embryonic development in zebrafish.

    PubMed

    Yamada, Toshiki; Wondergem, Robert; Morrison, Rebecca; Yin, Viravuth P; Strange, Kevin

    2016-10-01

    A volume-regulated anion channel (VRAC) has been electrophysiologically characterized in innumerable mammalian cell types. VRAC is activated by cell swelling and mediates the volume regulatory efflux of Cl(-) and small organic solutes from cells. Two groups recently identified the mammalian leucine-rich repeat containing protein LRRC8A as an essential VRAC component. LRRC8A must be coexpressed with at least one of the other four members of this gene family, LRRC8B-E, to reconstitute VRAC activity in LRRC8(-/-) cells. LRRC8 genes likely arose with the origin of chordates. We identified LRRC8A and LRRC8C-E orthologs in the zebrafish genome and demonstrate that zebrafish embryo cells and differentiated adult cell types express a swelling-activated Cl(-) current indistinguishable from mammalian VRAC currents. Embryo cell VRAC currents are virtually eliminated by morpholino knockdown of the zebrafish LRRC8A ortholog lrrc8aa VRAC activity is fully reconstituted in LRRC8(-/-) human cells by coexpression of zebrafish lrrc8aa and human LRRC8C cDNAs. lrrc8aa expression varies during zebrafish embryogenesis and lrrc8aa knockdown causes pericardial edema and defects in trunk elongation and somatogenesis. Our studies provide confirmation of the importance of LRRC8A in VRAC activity and establish the zebrafish as a model system for characterizing the molecular regulation and physiological roles of VRAC and LRRC8 proteins.

  5. Design, synthesis, molecular docking and biological evaluation of thiophen-2-iminothiazolidine derivatives for use against Trypanosoma cruzi.

    PubMed

    Silva-Júnior, E F; Silva, E P S; França, P H B; Silva, J P N; Barreto, E O; Silva, E B; Ferreira, R S; Gatto, C C; Moreira, D R M; Siqueira-Neto, J L; Mendonça-Júnior, F J B; Lima, M C A; Bortoluzzi, J H; Scotti, M T; Scotti, L; Meneghetti, M R; Aquino, T M; Araújo-Júnior, J X

    2016-09-15

    In this study, we designed and synthesized a series of thiophen-2-iminothiazolidine derivatives from thiophen-2-thioureic with good anti-Trypanosoma cruzi activity. Several of the final compounds displayed remarkable trypanocidal activity. The ability of the new compounds to inhibit the activity of the enzyme cruzain, the major cysteine protease of T. cruzi, was also explored. The compounds 3b, 4b, 8b and 8c were the most active derivatives against amastigote form, with significant IC50 values between 9.7 and 6.03μM. The 8c derivative showed the highest potency against cruzain (IC50=2.4μM). Molecular docking study showed that this compound can interact with subsites S1 and S2 simultaneously, and the negative values for the theoretical energy binding (Eb=-7.39kcal·mol(-1)) indicates interaction (via dipole-dipole) between the hybridized sulfur sp(3) atom at the thiazolidine ring and Gly66. Finally, the results suggest that the thiophen-2-iminothiazolidines synthesized are important lead compounds for the continuing battle against Chagas disease.

  6. Design, synthesis, molecular docking and biological evaluation of thiophen-2-iminothiazolidine derivatives for use against Trypanosoma cruzi.

    PubMed

    Silva-Júnior, E F; Silva, E P S; França, P H B; Silva, J P N; Barreto, E O; Silva, E B; Ferreira, R S; Gatto, C C; Moreira, D R M; Siqueira-Neto, J L; Mendonça-Júnior, F J B; Lima, M C A; Bortoluzzi, J H; Scotti, M T; Scotti, L; Meneghetti, M R; Aquino, T M; Araújo-Júnior, J X

    2016-09-15

    In this study, we designed and synthesized a series of thiophen-2-iminothiazolidine derivatives from thiophen-2-thioureic with good anti-Trypanosoma cruzi activity. Several of the final compounds displayed remarkable trypanocidal activity. The ability of the new compounds to inhibit the activity of the enzyme cruzain, the major cysteine protease of T. cruzi, was also explored. The compounds 3b, 4b, 8b and 8c were the most active derivatives against amastigote form, with significant IC50 values between 9.7 and 6.03μM. The 8c derivative showed the highest potency against cruzain (IC50=2.4μM). Molecular docking study showed that this compound can interact with subsites S1 and S2 simultaneously, and the negative values for the theoretical energy binding (Eb=-7.39kcal·mol(-1)) indicates interaction (via dipole-dipole) between the hybridized sulfur sp(3) atom at the thiazolidine ring and Gly66. Finally, the results suggest that the thiophen-2-iminothiazolidines synthesized are important lead compounds for the continuing battle against Chagas disease. PMID:27475533

  7. Structure–function analysis of myomaker domains required for myoblast fusion

    PubMed Central

    Millay, Douglas P.; Gamage, Dilani G.; Quinn, Malgorzata E.; Min, Yi-Li; Mitani, Yasuyuki; Bassel-Duby, Rhonda; Olson, Eric N.

    2016-01-01

    During skeletal muscle development, myoblasts fuse to form multinucleated myofibers. Myomaker [Transmembrane protein 8c (TMEM8c)] is a muscle-specific protein that is essential for myoblast fusion and sufficient to promote fusion of fibroblasts with muscle cells; however, the structure and biochemical properties of this membrane protein have not been explored. Here, we used CRISPR/Cas9 mutagenesis to disrupt myomaker expression in the C2C12 muscle cell line, which resulted in complete blockade to fusion. To define the functional domains of myomaker required to direct fusion, we established a heterologous cell–cell fusion system, in which fibroblasts expressing mutant versions of myomaker were mixed with WT myoblasts. Our data indicate that the majority of myomaker is embedded in the plasma membrane with seven membrane-spanning regions and a required intracellular C-terminal tail. We show that myomaker function is conserved in other mammalian orthologs; however, related family members (TMEM8a and TMEM8b) do not exhibit fusogenic activity. These findings represent an important step toward deciphering the cellular components and mechanisms that control myoblast fusion and muscle formation. PMID:26858401

  8. Leucine-rich repeat containing protein LRRC8A is essential for swelling-activated Cl- currents and embryonic development in zebrafish.

    PubMed

    Yamada, Toshiki; Wondergem, Robert; Morrison, Rebecca; Yin, Viravuth P; Strange, Kevin

    2016-10-01

    A volume-regulated anion channel (VRAC) has been electrophysiologically characterized in innumerable mammalian cell types. VRAC is activated by cell swelling and mediates the volume regulatory efflux of Cl(-) and small organic solutes from cells. Two groups recently identified the mammalian leucine-rich repeat containing protein LRRC8A as an essential VRAC component. LRRC8A must be coexpressed with at least one of the other four members of this gene family, LRRC8B-E, to reconstitute VRAC activity in LRRC8(-/-) cells. LRRC8 genes likely arose with the origin of chordates. We identified LRRC8A and LRRC8C-E orthologs in the zebrafish genome and demonstrate that zebrafish embryo cells and differentiated adult cell types express a swelling-activated Cl(-) current indistinguishable from mammalian VRAC currents. Embryo cell VRAC currents are virtually eliminated by morpholino knockdown of the zebrafish LRRC8A ortholog lrrc8aa VRAC activity is fully reconstituted in LRRC8(-/-) human cells by coexpression of zebrafish lrrc8aa and human LRRC8C cDNAs. lrrc8aa expression varies during zebrafish embryogenesis and lrrc8aa knockdown causes pericardial edema and defects in trunk elongation and somatogenesis. Our studies provide confirmation of the importance of LRRC8A in VRAC activity and establish the zebrafish as a model system for characterizing the molecular regulation and physiological roles of VRAC and LRRC8 proteins. PMID:27688432

  9. Synthesis of CNS active thyrotropin-releasing hormone (TRH)-like peptides: Biological evaluation and effect on cognitive impairment induced by cerebral ischemia in mice.

    PubMed

    Meena, Chhuttan L; Thakur, Avinash; Nandekar, Prajwal P; Sangamwar, Abhay T; Sharma, Shyam S; Jain, Rahul

    2015-09-01

    Thyrotropin-releasing hormone (TRH)-like peptides were synthesized by replacing critical histidine and pGlu residues in the native peptide. The peptides were evaluated in vitro for receptor binding activity assay and in the cell functional assay; the peptides exhibit selective basal signaling agonist behavior toward TRH-R2. For example, peptides 8a, 8b, 8c, 8 f, 8 h, 8 l and 12 d activated TRH-R2 with potency (EC50) of 0.53 μM, 0.048 μM, 0.05 μM, 0.006 μM, 0.31 μM, 0.034 μM and 0.004 μM, respectively. In contrast for signaling activation of TRH-R1, the same peptide required higher concentration of 19.35 μM, 3.98 μM, 2.54 μM, 0.287 μM, 11.28 μM, 0.986 μM and 0.944 μM, respectively. The results showed that peptides were 36.5, 82.9, 50.8, 47.8, 36.3, 32.6 and 235-fold selective to TRH-R2 receptor subtype. The peptides were investigated for CNS activity at 10 μmol/kg in pentobarbital-induced sleep assay study. Peptides 8c (16.5 ± 1.4 min) and 8l (16.5 ± 2.1 min) displayed excellent CNS activity. In an in vivo study, peptide 8c did not cause significant change in the rat plasma TSH levels. The peptide 8c was further investigated for neuroprotective potential, and significantly reduced infracts volume and neurological score in the focal cerebral ischemia model in mice. Peptide 8c also significantly lowered MDA levels, indicating reduction of oxidative and enhanced percentage cell survival in CA1 region, when compared to ischemic brain. PMID:26216015

  10. Geologic and well-construction data for the H-8 borehole complex near the proposed Waste Isolation Pilot Plant site, southeastern New Mexico

    USGS Publications Warehouse

    Wells, J.G.; Drellack, S.L.

    1982-01-01

    The H-8 complex, a group of three closely-spaced boreholes, is located 9 miles south of the proposed Waste Isolation Pilot Plant site in southeastern Eddy County, New Mexico. The holes were drilled during July, August, and September of 1979 to obtain geologic and hydrologic data to better define the regional ground-water-flow system. The geologic data presented in this report are part of a site-characterization study for the possible disposal of defense-associated radioactive wastes within salt beds of the Salado Formation of Permian age. The geologic data include detailed descriptions of cores, cuttings, and geophysical logs. Each borehole was designed to penetrate a distinct water-bearing zone: H-8a (total depth 505 feet) was completed just below the Magenta Dolomite Member of the Rustler Formation of Permian Age; H-8b (total depth 624 feet) was completed just belows the Culebra Dolomite Member of the Rustler Formation; and H-8c (total depth 808 feet) was completed just below the Rustler Formation-Salado Formation contact. The geologic units penetrated in borehole H-8c are surficial alluvium and eolian sand of Holocene age (0-4 feet); the Mescalero caliche (4-10 feet) and Gatuna Formation (10-153 feet) , both of Pleistocene age; and the Dewey Lake Red Beds (153-399 feet), the Rustler Formation (399-733 feet), and part of the Salado Formation penetrated by borehole H-8c is composed of residue from dissolution of halite and associated rocks, and the hydration of anhydrite to gypsum, indicating that the eastward-moving dissolution front on top of the Salado, found just to the west of the WIPP site, has reached the H-8 site. (USGS)

  11. Synthesis of novel ethyl 1-ethyl-6-fluoro-7-(fatty amido)-1,4-dihydro-4-oxoquinoline-3-carboxylate derivatives and their biological evaluation.

    PubMed

    Venepally, Vijayendar; Prasad, R B N; Poornachandra, Y; Kumar, C Ganesh; Jala, Ram Chandra Reddy

    2016-01-15

    A series of novel ethyl 1-ethyl-6-fluoro-7-(fatty amido)-1,4-dihydro-4-oxoquinoline-3-carboxylate derivatives were prepared through multistep synthesis. The key step in the synthesis was to obtain the C-7 fatty amide derivative. The azide was selectively formed at C-7 position using sodium azide at 60°C. Subsequently, the azide was reduced under mild conditions using zinc and ammonium chloride to form the corresponding amine. The synthesized derivatives were further subjected to biological evaluation studies like cytotoxicity against a panel of cancer cell lines such as DU145, A549, SKOV3, MCF7 and normal lung cells, IMR-90 as well as with antimicrobial and antioxidant activities. It was observed that the carboxylated quinolone derivatives with hexanoic (8a), octanoic (8b), lauric (8d) and myristic (8e) moieties exhibited promising cytotoxicity against all the tested cancer cell lines. The results also suggested that hexanoic acid-based fatty amide carboxylated quinolone derivative (8a) exhibited promising activity against both bacterial and fungal strains and significant antibacterial activity was observed against Staphylococcus aureus MTCC 96 (MIC value of 3.9μg/mL). The compound 8a also showed excellent anti-biofilm activity against Staphylococcus aureus MTCC 96 and Bacillus subtilis MTCC 121 with MIC values of 2.1 and 4.6μg/mL, respectively.

  12. Synthesis of novel ethyl 1-ethyl-6-fluoro-7-(fatty amido)-1,4-dihydro-4-oxoquinoline-3-carboxylate derivatives and their biological evaluation.

    PubMed

    Venepally, Vijayendar; Prasad, R B N; Poornachandra, Y; Kumar, C Ganesh; Jala, Ram Chandra Reddy

    2016-01-15

    A series of novel ethyl 1-ethyl-6-fluoro-7-(fatty amido)-1,4-dihydro-4-oxoquinoline-3-carboxylate derivatives were prepared through multistep synthesis. The key step in the synthesis was to obtain the C-7 fatty amide derivative. The azide was selectively formed at C-7 position using sodium azide at 60°C. Subsequently, the azide was reduced under mild conditions using zinc and ammonium chloride to form the corresponding amine. The synthesized derivatives were further subjected to biological evaluation studies like cytotoxicity against a panel of cancer cell lines such as DU145, A549, SKOV3, MCF7 and normal lung cells, IMR-90 as well as with antimicrobial and antioxidant activities. It was observed that the carboxylated quinolone derivatives with hexanoic (8a), octanoic (8b), lauric (8d) and myristic (8e) moieties exhibited promising cytotoxicity against all the tested cancer cell lines. The results also suggested that hexanoic acid-based fatty amide carboxylated quinolone derivative (8a) exhibited promising activity against both bacterial and fungal strains and significant antibacterial activity was observed against Staphylococcus aureus MTCC 96 (MIC value of 3.9μg/mL). The compound 8a also showed excellent anti-biofilm activity against Staphylococcus aureus MTCC 96 and Bacillus subtilis MTCC 121 with MIC values of 2.1 and 4.6μg/mL, respectively. PMID:26646219

  13. Crystal Structure of Bi

    SciTech Connect

    Borg, Stefan; Svensson, Goeran

    2001-02-15

    The room temperature structures of the two-layer Aurivillius phases Bi{sub 2.5}Me{sub 0.5}Nb{sub 2}O{sub 9} (Me=Na, K) have been refined with the Rietveld method from powder neutron diffraction data ({lambda}=1.470 {angstrom}). They consist of (Bi{sub 2}O{sub 2}){sup 2+} layers interleaved with perovskite (Bi{sub 0.5}Me{sub 0.5}Nb{sub 2}O{sub 7}){sup 2-} (Me=Na, K) slabs. The structures were refined in the orthorhombic space group A2{sub 1}am, Z=4, and the unit cell parameters of the two oxides are a= 5.4937(3), b=5.4571(4), c=24.9169(14) {angstrom} and a=5.5005(8), b=5.4958(8), c=25.2524(16) {angstrom}, respectively. The orthorhombic distortion increases with decreasing Me+ cation size in the perovskite layer (Bi/Me){sup 2+} site and the lone pair electrons from the Bi{sup 3+} cation are influencing the site distortion. This is in agreement with other two-layer Aurivillius phases and originates from bonding requirements depending on size and electronic environment.

  14. Survey of the terrestrial habitats and vegetation of Shetland, 1974 - a framework for long-term ecological monitoring

    NASA Astrophysics Data System (ADS)

    Wood, Claire M.; Bunce, Robert G. H.

    2016-02-01

    A survey of the natural environment was undertaken in Shetland in 1974, after concern was expressed that large-scale development from the new oil industry could threaten the natural features of the islands. A framework was constructed by the Institute of Terrestrial Ecology on which to select samples for the survey. The vegetation and habitat data that were collected, along with the sampling framework, have recently been made public via the following doi:10.5285/06fc0b8c-cc4a-4ea8-b4be-f8bd7ee25342 (Terrestrial habitat, vegetation and soil data from Shetland, 1974) and doi:10.5285/f1b3179e-b446-473d-a5fb-4166668da146 (Land Classification of Shetland 1974). In addition to providing valuable information about the state of the natural environment of Shetland, the repeatable and statistically robust methods developed in the survey were used to underpin the Countryside Survey, Great Britain's national long-term integrated environmental monitoring programme. The demonstration of the effectiveness of the methodology indicates that a repeat of the Shetland survey would yield statistics about ecological changes in the islands, such as those arising from the impacts of the oil industry, a range of socio-economic impacts, and perhaps climate change. Currently no such figures are available, although there is much information on the sociological impacts, as well as changes in agriculture.

  15. Imaginary part of the 9C-9Be single-folded optical potential

    NASA Astrophysics Data System (ADS)

    Bonaccorso, A.; Carstoiu, F.; Charity, R. J.

    2016-09-01

    In a recent publication we have argued that using two very successful n -9Be optical potentials [A. Bonaccorso and R. J. Charity, Phys. Rev. C 89, 024619 (2014), 10.1103/PhysRevC.89.024619] and microscopic projectile densities, it is possible to build a single-folded (light-) nucleus-9Be imaginary optical potential which is more accurate than a double-folded optical potential. By comparing to experimental reaction cross sections, we showed for 8B,8Li, and 8C projectiles, that a very good agreement between theory and data could be obtained with such a "bare" potential, at all but the lowest energies where a small semimicroscopic surface term was added to the single-folded potential to take into account projectile breakup. In this paper we extend this study to the case of 9C projectiles and assess the sensitivity to the projectile density used. We then obtained the modulus of the nucleus-nucleus S matrix and parametrize it in terms of a strong-absorption radius Rs and finally extracted the phenomenological energy dependence of this radius. This approach could be the basis for a systematic study of optical potentials for light exotic nuclei scattering on light targets and/or parametrizations of the S matrix. Furthermore our study will serve to make a quantitative assessment of the description of the core-target part of knockout reactions, in particular their localization in terms of impact parameters.

  16. Synthesis and structures of YNiIn 2 and Y 4Ni 11In 20

    NASA Astrophysics Data System (ADS)

    Hlukhyy, Viktor; Zaremba, Vasyl'I.; Kalychak, Yaroslav M.; Pöttgen, Rainer

    2004-04-01

    Well-shaped single crystals of YNiIn 2 and Y 4Ni 11In 20 were obtained by arc-melting of the elements and subsequent slow cooling. Both indides were investigated by X-ray diffraction on powders and single crystals: MgCuAl 2 type, Cmcm, a=431.52(8), b=1042.0(2), c=730.0(1) pm, w R2=0.0471, 587 F2 values, 16 variables for YNiIn 2 and U 4Ni 11Ga 20 type, C2/ m, a=2251.2(4), b=430.77(8), c=1658.5(3) pm, β=124.62(1)°, w R2=0.0542, 1583 F2 values, 108 variables for Y 4Ni 11In 20. Both structures are built up from three-dimensional [NiIn 2] and [Ni 11In 20] networks in which the yttrium atoms fill distorted pentagonal channels. The [NiIn 2] network has only Ni-In and In-In contacts, while also Ni-Ni bonding plays an important role in the [Ni 11In 20] network.

  17. SILAC based protein profiling data of MKK3 knockout mouse embryonic fibroblasts.

    PubMed

    Srivastava, Anup; Shinn, Amanda S; Lam, TuKiet T; Lee, Patty J; Mannam, Praveen

    2016-06-01

    This data article reports changes in the phospho and total proteome of MKK3 knock out (MKK3(-) (/) (-)) mouse embryonic fibroblasts (MEFs). The dataset generated highlights the changes at protein level which can be helpful for understanding targets of the MAP kinase signaling pathway. Data was collected after TiO2-based phosphopeptide enrichment of whole cell lysate at baseline condition with bottom-up SILAC-based LC MS/MS quantitative mass spectrometry. We report all the proteins and peptides identified and quantified in MKK3(-/-) and WT MEFs. The altered pathways in MKK3(-/-) MEFs were analyzed by Database for Annotation, Visualization and Integrated Discovery (DAVID, v6.7) and Ingenuity Pathway Analysis (IPA) and are presented as a table and graph, respectively. The data reported here is related to the published work [1]. All the associated mass spectrometry data has been deposited in the Yale Protein Expression Database (YPED) with the web-link to the data: http://yped.med.yale.edu/repository/ViewSeriesMenu.do;jsessionid=6A5CB07543D8B529FAE8C3FCFE29471D?series_id=5044&series_name=MMK3+Deletion+in+MEFs. PMID:26977448

  18. Activation of Single-Component Nickel(II) Polyethylene Catalysts via Phase Transfer of Fluorous Phosphine Ligands.

    PubMed

    Xi, Zhenxing; Bazzi, Hassan S; Gladysz, John A

    2015-09-01

    The nickel salicylaldiminato phosphine complexes [1,2,3-C6H3(9-anthracenyl)O(CH═N(2,6-C6H3(iPr)2)]Ni(Me)[P(4-C6H4R)3] (4; R = a, (CH2)2Rf8; b, (CH2)3Rf8; c, H (Rf8 = (CF2)7CF3)) are prepared from the corresponding phosphines 3a-c and nickel NCMe adduct (46-68%). These are applied as catalysts for ethylene polymerization in toluene and fluorous/toluene liquid/liquid biphasic mixtures. Under the latter conditions, the fluorous phosphines 3a,b that must dissociate to generate the active catalyst migrate to the fluorous phase (partition coefficients 97.5:2.5 and 66.6:33.4 vs <0.5:>99.5 for 4a,b). Catalysts 4a,b show marked accelerations under biphasic conditions, but 4c (which has a lipophilic phosphine ligand) does not. Under all conditions, 4a,b are faster catalysts than the Ni(Ph)(PPh3) analogue, a previously reported benchmark. PMID:26300472

  19. Long-term variations of atmospheric wave activity in the mesosphere and lower thermosphere region over the equatorial Pacific

    NASA Astrophysics Data System (ADS)

    Tsuda, T.; Yoshida, S.; Isoda, F.; Nakamura, T.; Nuryanto, A.; Manurung, S.; Sobari, O.; Vincent, R. A.; Reid, I. M.

    2002-05-01

    We have analyzed long-term variations of the wind velocity variance of ultra-fast Kelvin (UFK) waves (3.0-3.8d) and gravity waves in two wave period ranges; 30-35h (0.7-0.8c/d) and 8-11h(2.1-2.9c/d), in the equatorial mesosphere and lower thermosphere region using meteor wind radar (MWR) observations in Jakarta (JKT) (6°S107°E) from November 1992 to June 1998, as well as two medium frequency (MF) radars at Christmas Island (2°N157°W) and Pontianak (0.03°N109°E) from January 1996 to July 1997. The long-term variations of the wind variance of these waves at JKT showed enhancements twice a year in 1993-1995, mostly coinciding with the westward wind phase of MSAO or the transition period of MSAO from eastward to westward wind. The activity of both UFK and gravity waves became significantly weak in 1996-1997. In particular, the UFK wave variance became simultaneously smaller at JKT and the two MF radar sites, indicating that this peculiar event had a global scale.

  20. Novel Coumarin-Containing Aminophosphonatesas Antitumor Agent: Synthesis, Cytotoxicity, DNA-Binding and Apoptosis Evaluation.

    PubMed

    Li, Ya-Jun; Wang, Cai-Yi; Ye, Man-Yi; Yao, Gui-Yang; Wang, Heng-Shan

    2015-01-01

    A series of novel coumarin-containing α-aminophosphonates were synthesized and evaluated for their antitumor activities against Human colorectal (HCT-116), human nasopharyngeal carcinoma (human KB) and human lung adenocarcinoma (MGC-803) cell lines in vitro. Compared with 7-hydroxy-4-methylcoumarin (4-MU), most of the derivatives showed an improved antitumor activity. Compound 8j (diethyl 1-(3-(4-methyl-2-oxo-2H-chromen-7-yloxy) propanamido)-1-phenylethyl-Phosphonate), with IC50 value of 8.68 μM against HCT-116 cell lines, was about 12 fold than that of unsubstituted parent compound. The mechanism investigation proved that 8c, 8d, 8f and 8j were achieved through the induction of cell apoptosis by G1 cell-cycle arrest. In addition, the further mechanisms of compound 8j-induced apoptosis in HCT-116 cells demonstrated that compound 8j induced the activations of caspase-9 and caspase-3 for causing cell apoptosis, and altered anti- and pro-apoptotic proteins. DNA-binding experiments suggested that some derivatives bind to DNA through intercalation. The results seem to imply the presence of an important synergistic effect between coumarin and aminophosphonate, which could contribute to the strong chelating properties of aminophosphonate moiety.

  1. Synthesis and biological activities of novel 5-substituted-1,3,4-oxadiazole Mannich bases and bis-Mannich bases as ketol-acid reductoisomerase inhibitors.

    PubMed

    Zhang, Yan; Liu, Xing-Hai; Zhan, Yi-Zhou; Zhang, Li-Yuan; Li, Zheng-Ming; Li, Yong-Hong; Zhang, Xiao; Wang, Bao-Lei

    2016-10-01

    A series of novel 5-substituted-1,3,4-oxadiazole Mannich bases and bis-Mannich bases have been conveniently synthesized in good yields. Their structures were characterized by IR, (1)H NMR, (13)C NMR and elemental analysis. The preliminary bioassay results indicated that some of the compounds showed promising in vitro fungicidal activities towards several test plant fungi; some of them exhibited significant herbicidal activities against Brassica campestris and excellent in vitro inhibitory activities against rice ketol-acid reductoisomerase (KARI). Among 14 novel compounds, 8c, 8d and 8m showed potent KARI inhibitory activities with Ki value of (0.96±0.42), (3.86±0.49) and (3.10±0.71) μmol/L, respectively, and were comparable with IpOHA. These compounds could be novel KARI inhibitors for further investigation. The density functional theory (DFT) calculations and molecular docking were carried out to study the structure-activity relationship (SAR) of the active inhibitors in this Letter. PMID:27575481

  2. Design, synthesis, antimicrobial evaluation and molecular docking studies of some new 2,3-dihydrothiazoles and 4-thiazolidinones containing sulfisoxazole.

    PubMed

    Nasr, Tamer; Bondock, Samir; Eid, Sameh

    2016-01-01

    Microbial resistance to the available drugs poses a serious threat in modern medicine. We report the design, synthesis and in vitro antimicrobial evaluation of new functionalized 2,3-dihydrothiazoles and 4-thiazolidinones tagged with sulfisoxazole moiety. Compound 8d was most active against Bacillis subtilis (MIC, 0.007 µg/mL). Moreover, compounds 7c-d and 8c displayed significant activities against B. subtilis and Streptococcus pneumoniae (MIC, 0.03-0.06 µg/mL and 0.06-0.12 µg/mL versus ampicillin 0.24 µg/mL and 0.12 µg/mL; respectively). Compounds 7a and 7c-d were highly potent against Escherichia coli (MIC, 0.49-0.98 µg/mL versus gentamycin 1.95 µg/mL). On the other hand, compounds 7e and 9c were fourfolds more active than amphotericin B against Syncephalastrum racemosum. Molecular docking studies showed that the synthesized compounds could act as inhibitors for the dihydropteroate synthase enzyme (DHPS). This study is a platform for the future design of more potent antimicrobial agents.

  3. 21 CFR 172.710 - Adjuvants for pesticide use dilutions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... blends that are added to herbicides for application to corn and sorghum. Di-n-alkyl (C8-C18) dimethyl ammonium chloride, where the alkyl groups (C8-C18) are derived from coconut oil, as surfactants in... growing crop: n-Alkyl (C8-C18) amine acetate, where the alkyl groups (C8-C18) are derived from coconut...

  4. Knockout beyond the dripline

    SciTech Connect

    Bonaccorso, A.; Charity, R. J.; Kumar, R.; Salvioni, G.

    2015-02-24

    In this contribution, we will describe neutron and proton removal from {sup 9}C and {sup 7}Be which are two particularly interesting nuclei entering the nucleo-synthesis pp-chain [1, 2]. Neutron and proton removal reactions have been used in the past twenty years to probe the single-particle structure of exotic nuclei. The core parallel-momentum distribution can give information on the angular momentum and spin of the nucleon initial state while the total removal cross section is sensitive to the asymptotic part of the initial wave function and also to the reaction mechanism. Because knockout is a peripheral reaction from which the Asymptotic Normalization Constant (ANC) of the single-particle wave function can be extracted, it has been used as an indirect method to obtain the rate of reactions like {sup 8}B(p,γ){sup 9}C or {sup 7}Be(p,γ){sup 8}B. Nucleon removal has recently been applied by the HiRA collaboration [3] to situations in which the remaining “core” is beyond the drip line, such as {sup 8}C and {sup 6}Be, unbound by one or more protons, and whose excitation-energy spectrum can be obtained by the invariant-mass method. By gating on the ground-state peak, “core” parallel-momentum distributions and total knockout cross sections have been obtained similar to previous studies with well-bound “cores”. In addition for each projectile, knock out to final bound states has also been obtained in several cases. We will report on the theoretical description and comparison to this experimental data for a few cases for which advances in the accuracy of the transfer-to-the continuum model [4, 5] have been made [6]. These include the use, when available, of “ab-initio” overlaps for the initial state [7] and in particular their ANC values [8]. Also, the construction of a nucleus-target folding potential for the treatment of the core-target S-matrix [9] using for the cores “ab-initio” densities [10] and state-of-the-art n−{sup 9}Be optical

  5. Base-Displaced Intercalated Conformation of the 2-Amino-3-methylimidazo[4,5-f]quinoline N2-dG DNA Adduct Positioned at the Nonreiterated G1 in the NarI Restriction Site

    PubMed Central

    2016-01-01

    The conformation of an N2-dG adduct arising from the heterocyclic amine 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), a potent food mutagen, was determined in 5′-d(C1T2C3X4G5C6G7C8C9A10T11C12)-3′:5′-d(G13A14T15G16G17C18G19C20C21G22A23G24)-3′; X = N2-dG-IQ, in which the modified nucleotide X4 corresponds to G1 in the 5′-d(G1G2CG3CC)-3′ NarI restriction endonuclease site. Circular dichroism (CD) revealed blue shifts relative to the unmodified duplex, consistent with adduct-induced twisting, and a hypochromic effect for the IQ absorbance in the near UV region. NMR revealed that the N2-dG-IQ adduct adopted a base-displaced intercalated conformation in which the modified guanine remained in the anti conformation about the glycosidic bond, the IQ moiety intercalated into the duplex, and the complementary base C21 was displaced into the major groove. The processing of the N2-dG-IQ lesion by hpol η is sequence-dependent; when placed at the reiterated G3 position, but not at the G1 position, this lesion exhibits a propensity for frameshift replication [Choi, J. Y., et al. (2006) J. Biol. Chem., 281, 25297–25306]. The structure of the N2-dG-IQ adduct at the nonreiterated G1 position was compared to that of the same adduct placed at the G3 position [Stavros, K. M., et al. (2014) Nucleic Acids Res., 42, 3450–3463]. CD indicted minimal spectral differences between the G1 vs G3N2-dG-IQ adducts. NMR indicated that the N2-dG-IQ adduct exhibited similar base-displaced intercalated conformations at both the G1 and G3 positions. This result differed as compared to the corresponding C8-dG-IQ adducts placed at the same positions. The C8-dG-IQ adduct adopted a minor groove conformation when placed at position G1 but a base-displaced intercalated conformation when placed at position G3 in the NarI sequence. The present studies suggest that differences in lesion bypass by hpol η may be mediated by differences in the 3′-flanking sequences, perhaps modulating the ability

  6. Peramivir Phosphonate Derivatives as Influenza Neuraminidase Inhibitors.

    PubMed

    Wang, Peng-Cheng; Fang, Jim-Min; Tsai, Keng-Chang; Wang, Shi-Yun; Huang, Wen-I; Tseng, Yin-Chen; Cheng, Yih-Shyun E; Cheng, Ting-Jen Rachel; Wong, Chi-Huey

    2016-06-01

    Peramivir is a potent neuraminidase (NA) inhibitor for treatment of influenza infection by intravenous administration. By replacing the carboxylate group in peramivir with a phosphonate group, phosphono-peramivir (6a), the dehydration and deoxy derivatives (7a and 8a) as well as their corresponding monoalkyl esters are prepared from a pivotal intermediate epoxide 12. Among these phosphonate compounds, the dehydration derivative 7a that has a relatively rigid cyclopentene core structure exhibits the strongest inhibitory activity (IC50 = 0.3-4.1 nM) against several NAs of wild-type human and avian influenza viruses (H1N1, H3N2, H5N1, and H7N9), although the phosphonate congener 6a is unexpectedly less active than peramivir. The inferior binding affinity of 6a is attributable to the deviated orientations of its phosphonic acid and 3-pentyl groups in the NA active site as inferred from the NMR, X-ray diffraction, and molecular modeling analyses. Compound 7a is active to the oseltamivir-resistant H275Y strains of H1N1 and H5N1 viruses (IC50 = 73-86 nM). The phosphonate monoalkyl esters (6b, 6c, 7b, 7c, 8b, and 8c) are better anti-influenza agents (EC50 = 19-89 nM) than their corresponding phosphonic acids (EC50 = 50-343 nM) in protection of cells from the viral infection. The phosphonate monoalkyl esters are stable in buffer solutions (pH 2.0-7.4) and rabbit serum; furthermore, the alkyl group is possibly tuned to attain the desired pharmacokinetic properties. PMID:27167096

  7. New techniques for imaging and analyzing lung tissue.

    PubMed Central

    Roggli, V L; Ingram, P; Linton, R W; Gutknecht, W F; Mastin, P; Shelburne, J D

    1984-01-01

    The recent technological revolution in the field of imaging techniques has provided pathologists and toxicologists with an expanding repertoire of analytical techniques for studying the interaction between the lung and the various exogenous materials to which it is exposed. Analytical problems requiring elemental sensitivity or specificity beyond the range of that offered by conventional scanning electron microscopy and energy dispersive X-ray analysis are particularly appropriate for the application of these newer techniques. Electron energy loss spectrometry, Auger electron spectroscopy, secondary ion mass spectrometry, and laser microprobe mass analysis each offer unique advantages in this regard, but also possess their own limitations and disadvantages. Diffraction techniques provide crystalline structural information available through no other means. Bulk chemical techniques provide useful cross-checks on the data obtained by microanalytical approaches. It is the purpose of this review to summarize the methodology of these techniques, acknowledge situations in which they have been used in addressing problems in pulmonary toxicology, and comment on the relative advantages and disadvantages of each approach. It is necessary for an investigator to weigh each of these factors when deciding which technique is best suited for any given analytical problem; often it is useful to employ a combination of two or more of the techniques discussed. It is anticipated that there will be increasing utilization of these technologies for problems in pulmonary toxicology in the decades to come. Images FIGURE 3. A FIGURE 3. B FIGURE 3. C FIGURE 3. D FIGURE 4. FIGURE 5. FIGURE 7. A FIGURE 7. B FIGURE 8. A FIGURE 8. B FIGURE 8. C FIGURE 9. A FIGURE 9. B FIGURE 10. PMID:6090115

  8. Biotransformations of Bile Acids with Bacteria from Cayambe Slaughterhouse (Ecuador): Synthesis of Bendigoles.

    PubMed

    Costa, Stefania; Maldonado Rodriguez, Maria Elena; Rugiero, Irene; De Bastiani, Morena; Medici, Alessandro; Tamburini, Elena; Pedrini, Paola

    2016-08-01

    The biotransformations of cholic acid (1a), deoxycholic acid (1b), and hyodeoxycholic acid (1c) to bendigoles and other metabolites with bacteria isolated from the rural slaughterhouse of Cayambe (Pichincha Province, Ecuador) were reported. The more active strains were characterized, and belong to the genera Pseudomonas and Rhodococcus. Various biotransformation products were obtained depending on bacteria and substrates. Cholic acid (1a) afforded the 3-oxo and 3-oxo-4-ene derivatives 2a and 3a (45% and 45%, resp.) with P. mendocina ECS10, 3,12-dioxo-4-ene derivative 4a (60%) with Rh. erythropolis ECS25, and 9,10-secosteroid 6 (15%) with Rh. erythropolis ECS12. Bendigole F (5a) was obtained in 20% with P. fragi ECS22. Deoxycholic acid (1b) gave 3-oxo derivative 2b with P. prosekii ECS1 and Rh. erythropolis ECS25 (20% and 61%, resp.), while 3-oxo-4-ene derivative 3b was obtained with P. prosekii ECS1 and P. mendocina ECS10 (22% and 95%, resp.). Moreover, P. fragi ECS9 afforded bendigole A (8b; 80%). Finally, P. mendocina ECS10 biotransformed hyodeoxycholic acid (1c) to 3-oxo derivative 2c (50%) and Rh. erythropolis ECS12 to 6α-hydroxy-3-oxo-23,24-dinor-5β-cholan-22-oic acid (9c, 66%). Bendigole G (5c; 13%) with P. prosekii ECS1 and bendigole H (8c) with P. prosekii ECS1 and Rh. erythropolis ECS12 (20% and 16%, resp.) were obtained. PMID:27358241

  9. Multiple mean motion resonances in the HR 8799 planetary system

    NASA Astrophysics Data System (ADS)

    Goździewski, Krzysztof; Migaszewski, Cezary

    2014-06-01

    HR 8799 is a nearby star hosting at least four ˜10 mJup planets in wide orbits up to ˜70 au, detected through the direct, high-contrast infrared imaging. Large companions and debris discs reported interior to ˜10 au, and exterior to ˜100 au indicate massive protoplanetary disc in the past. The dynamical state of the HR 8799 system is not yet fully resolved, due to limited astrometric data covering tiny orbital arcs. We construct a new orbital model of the HR 8799 system, assuming rapid migration of the planets after their formation in wider orbits. We found that the HR 8799 planets are likely involved in double Laplace resonance, 1e:2d:4c:8b MMR. Quasi-circular planetary orbits are coplanar with the stellar equator and inclined by ˜25° to the sky plane. This best-fitting orbital configuration matches astrometry, debris disc models, and mass estimates from cooling models. The multiple mean motion resonance (MMR) is stable for the age of the star ˜160 Myr, for at least 1 Gyr unless significant perturbations to the N-body dynamics are present. We predict four configurations with the fifth hypothetical innermost planet HR 8799f in ˜9.7 au, or ˜7.5 au orbit, extending the MMR chain to triple Laplace resonance 1f:2e:4d:8c:16b MMR or to the 1f:3e:6d:12c:24b MMR, respectively. Our findings may establish strong boundary conditions for the system formation and its early history.

  10. Descriptions of Deladenus albizicus n. sp. and D. processus n. sp. (Nematoda: Hexatylina) from Haryana, India

    PubMed Central

    Tomar, V. V. S.; Somvanshi, Vishal S.; Bajaj, Harish K.

    2015-01-01

    Two different nematodes were isolated from the bark of Albizia lebbeck trees; one from insect infested and another from noninfested, healthy tree. Based on the biological, morphological, and molecular evidences, the nematodes are described as Deladenus albizicus n. sp. and D. processus n. sp. (Nematoda: Hexatylina). Deladenus albizicus n. sp., isolated from insect-infested tree, multiplied on the fungus Nigrospora oryzae. Myceliophagous females of this nematode reproduced by parthenogenesis and spermathecae were indistinct. Infective females, readily produced in the cultures, are dorsally curved. Only one type of males containing small-sized sperms in their genital tracts were produced in the culture. Myceliophagous females: L = 0.75 to 1.71 mm, a = 32.3 to 50.8, b = 9.3 to 11.2, b’ = 5.2 to 7.3, c = 27.2 to 35.6, V = 91.0 to 93.3, c’ = 2.0 to 2.9, stylet = 11 to 12 µm, excretory pore in the region of median pharyngeal bulb, 43 to 47 µm anterior to hemizonid. Deladenus processus n. sp., isolated from bark of healthy A. lebbeck tree, was cultured on Alternaria alternata. Myceliophagous females reproduced by amphimixis and their spermathecae contained rounded sperms. Infective females were never produced, even in old cultures. Myceliophagous females: L = 0.76 to 0.99 mm, a = 34 to 49, b = 13.3 to 17.7, b’ = 3.8 to 5.8, c = 19.6 to 22.8, V = 92.2 to 93.5, c’ = 2.7 to 3.5, stylet = 6 to 7 µm, excretory pore in the proximity of hemizonid, tail conoid, tapering from both sides to a long pointed central process. It is proposed to classify Deladenus species in three groups: durus, siricidicola, and laricis groups based on female and spermatogonia dimorphism, mode of reproduction, and insect parasitism. PMID:25861116

  11. Effect of Regular Resistance Training on Motivation, Self-Perceived Health, and Quality of Life in Previously Inactive Overweight Women: A Randomized, Controlled Trial

    PubMed Central

    Rustaden, Anne Mette; Bø, Kari; Haakstad, Lene A. H.

    2016-01-01

    Objectives. The aim was to investigate the effects of three different types of resistance training implementation. Design. Randomized controlled trial. Methods. Inactive, overweight women (n = 143), mean BMI 31.3 ± 5.2 kg/m2, mean age 39.9 ± 10.5 years, were randomized to one of the following groups: A (BodyPump group training), B (individual follow-up by a personal trainer), C (nonsupervised exercise), or D (controls). The intervention included 12 weeks of 45–60 minutes' full-body resistance training three sessions per week. The outcomes in this paper are all secondary outcome measures: exercise motivation, self-perceived health, and quality of life. Results. Adherence averaged 26.1 ± 10.3 of 36 prescribed sessions. After the intervention period, all three training groups (A–C) had better scores on exercise motivation (A = 43.9 ± 19.8, B = 47.6 ± 15.4, C = 48.4 ± 17.8) compared to the control group (D) (26.5 ± 18.2) (p < 0.001). Groups B and C scored better on self-perceived health (B = 1.9 ± 0.8, C = 2.3 ± 0.8), compared to group D (3.0 ± 0.6) (p < 0.001). For quality of life measurement, there was no statistically significant difference between either intervention groups or the control. Conclusions. Resistance training contributed to higher scores in important variables related to exercise motivation and self-perceived health. Low adherence showed that it was difficult to motivate previously inactive, overweight women to participate in regular strength training. PMID:27462608

  12. Effect of Regular Resistance Training on Motivation, Self-Perceived Health, and Quality of Life in Previously Inactive Overweight Women: A Randomized, Controlled Trial.

    PubMed

    Heiestad, Hege; Rustaden, Anne Mette; Bø, Kari; Haakstad, Lene A H

    2016-01-01

    Objectives. The aim was to investigate the effects of three different types of resistance training implementation. Design. Randomized controlled trial. Methods. Inactive, overweight women (n = 143), mean BMI 31.3 ± 5.2 kg/m(2), mean age 39.9 ± 10.5 years, were randomized to one of the following groups: A (BodyPump group training), B (individual follow-up by a personal trainer), C (nonsupervised exercise), or D (controls). The intervention included 12 weeks of 45-60 minutes' full-body resistance training three sessions per week. The outcomes in this paper are all secondary outcome measures: exercise motivation, self-perceived health, and quality of life. Results. Adherence averaged 26.1 ± 10.3 of 36 prescribed sessions. After the intervention period, all three training groups (A-C) had better scores on exercise motivation (A = 43.9 ± 19.8, B = 47.6 ± 15.4, C = 48.4 ± 17.8) compared to the control group (D) (26.5 ± 18.2) (p < 0.001). Groups B and C scored better on self-perceived health (B = 1.9 ± 0.8, C = 2.3 ± 0.8), compared to group D (3.0 ± 0.6) (p < 0.001). For quality of life measurement, there was no statistically significant difference between either intervention groups or the control. Conclusions. Resistance training contributed to higher scores in important variables related to exercise motivation and self-perceived health. Low adherence showed that it was difficult to motivate previously inactive, overweight women to participate in regular strength training. PMID:27462608

  13. NMR structural studies of the ionizing radiation adduct 7-hydro-8-oxodeoxyguanosine (8-oxo-7H-dG) opposite deoxyadenosine in a DNA duplex. 8-Oxo-7H-dG(syn).dA(anti) alignment at lesion site.

    PubMed

    Kouchakdjian, M; Bodepudi, V; Shibutani, S; Eisenberg, M; Johnson, F; Grollman, A P; Patel, D J

    1991-02-01

    Proton NMR studies are reported on the complementary d(C1-C2-A3-C4-T5-A6-oxo-G7-T8-C9-A10-C11-C12).d(G13-G14-T15- G16-A17-A18-T19- A20-G21-T22-G23-G24) dodecanucleotide duplex (designated 8-oxo-7H-dG.dA 12-mer), which contains a centrally located 7-hydro-8-oxodeoxyguanosine (8-oxo-7H-dG) residue, a group commonly found in DNA that has been exposed to ionizing radiation or oxidizing free radicals. From the NMR spectra it can be deduced that this moiety exists as two tautomers, or gives rise to two DNA conformations, that are in equilibrium and that exchange slowly. The present study focuses on the major component of the equilibrium that originates in the 6,8-dioxo tautomer of 8-oxo-7H-dG. We have assigned the exchangeable NH1, NH7, and NH2-2 base protons located on the Watson-Crick and Hoogsteen edges of 8-oxo-7H-dG7 in the 8-oxo-7H-dG.dA 12-mer duplex, using an analysis of one- and two-dimensional nuclear Overhauser enhancement (NOE) data in H2O solution. The observed NOEs derived from the NH7 proton of 8-oxo-7H-dG7 to the H2 and NH2-6 protons of dA18 establish an 8-oxo-7H-dG7(syn).dA 18(anti) alignment at the lesion site in the 8-oxo-7H-dG.dA 12-mer duplex in solution. This alignment, which places the 8-oxo group in the minor groove, was further characterized by an analysis of the NOESY spectrum of the 8-oxo-7H-dG.dA 12-mer duplex in D2O solution. We were able to detect a set of intra- and interstrand NOEs between protons (exchangeable and nonexchangeable) on adjacent residues in the d(A6-oxo-G7-T8).d(A17-A18-T19) trinucleotide segment centered about the lesion site that establishes stacking of the oxo-dG7(syn).dA(anti) pair between stable Watson-Crick dA6.dT19 and dT8.dA17 base pairs with minimal perturbation of the helix. Thus, both strands of the 8-oxo-7H-dG.dA 12-mer duplex adopt right-handed conformations at and adjacent to the lesion site, the unmodified bases adopt anti glycosidic torsion angles, and the bases are stacked into the helix. The energy

  14. 26 CFR 1.404(a)(8)-1T - Deductions for plan contributions on behalf of self-employed individuals. (Temporary)

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... by section 713(d)(6) of the Tax Reform Act of 1984 (TRA of 1984), affect section 404(a)(8)(C)? A: In... also having been made to section 404(a)(8)(C), pending enactment of technical corrections to TRA...

  15. 26 CFR 1.404(a)(8)-1T - Deductions for plan contributions on behalf of self-employed individuals. (Temporary)

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... by section 713(d)(6) of the Tax Reform Act of 1984 (TRA of 1984), affect section 404(a)(8)(C)? A: In... also having been made to section 404(a)(8)(C), pending enactment of technical corrections to TRA...

  16. 26 CFR 1.404(a)(8)-1T - Deductions for plan contributions on behalf of self-employed individuals. (Temporary)

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... section 713(d)(6) of the Tax Reform Act of 1984 (TRA of 1984), affect section 404(a)(8)(C)? A: In applying... also having been made to section 404(a)(8)(C), pending enactment of technical corrections to TRA...

  17. 26 CFR 1.404(a)(8)-1T - Deductions for plan contributions on behalf of self-employed individuals. (Temporary)

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... by section 713(d)(6) of the Tax Reform Act of 1984 (TRA of 1984), affect section 404(a)(8)(C)? A: In... also having been made to section 404(a)(8)(C), pending enactment of technical corrections to TRA...

  18. 26 CFR 1.404(a)(8)-1T - Deductions for plan contributions on behalf of self-employed individuals. (Temporary)

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... by section 713(d)(6) of the Tax Reform Act of 1984 (TRA of 1984), affect section 404(a)(8)(C)? A: In... also having been made to section 404(a)(8)(C), pending enactment of technical corrections to TRA...

  19. 21 CFR 172.710 - Adjuvants for pesticide use dilutions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... and sorghum. Di-n-alkyl (C8-C18) dimethyl ammonium chloride, where the alkyl groups (C8-C18) are... applicant prior to application to the growing crop: n-Alkyl (C8-C18) amine acetate, where the alkyl...

  20. 21 CFR 172.710 - Adjuvants for pesticide use dilutions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... and sorghum. Di-n-alkyl (C8-C18) dimethyl ammonium chloride, where the alkyl groups (C8-C18) are... applicant prior to application to the growing crop: n-Alkyl (C8-C18) amine acetate, where the alkyl...