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Sample records for 96-well plate system

  1. Electrothermal micromixing in 96 well plate

    NASA Astrophysics Data System (ADS)

    Kauffmann, Paul; Loire, Sophie; Mezic, Igor

    2011-11-01

    Diagnostic and pharmacology processes could be greatly accelerated by appropriate mixing. Here electrothermal flows are explored to provide mixing of conductive physiological solutions (=1.6 S/m) in a 96 well plate. Three interdigitated electrodes provide an electric field (< 15Vpp, 1MHz) beneath each well. Polarization and conduction phenomenon of the fluid in a well will be first modeled numerically and compared to an electrical circuit model. Due to high conductivity and permittivity of the fluid, the impedance of the array of filled wells collapse dramatically (96 wells: R = 1Ohm, C=250nF). The power supply challenges accordingly raised by arrays of electrothermal micromixers will be then analyzed. The efficiency of different methods of mixing in those wells will be also compared: the addition of low frequency signal leading to AC electro-osmotic perturbations, a blinking vortices method. The experimental results will be compared to simulations.

  2. An automated 96-well-plate loader for FACScan.

    PubMed

    Illges, H

    1999-01-01

    Staining multiple samples for data acquisition with a flow cytometer is done in 96-well plates to save time and make large data assessment in one working day possible. However, the inability of the FACScan to take up the samples from 96-well plates is a major drawback. In order to avoid the individual transfer of samples to tubes, we have developed a system, which allows using the FACScan with 96-well plates. The machine consists of a programmable control module and a loader which moves the 96-well plate in 3 axis well by well along the sample collector. The machine is equipped with a wash buffer tank to avoid cross contamination of samples and a shaking option to avoid sedimentation of cells during acquisition. The machine can be further developed into a full automatic loader if connected to the FACS Station. In 24 hours about 7,000 samples with 10,000 cells each can be acquired.

  3. Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System.

    PubMed

    Conway, Michael K; Gerger, Michael J; Balay, Erin E; O'Connell, Rachel; Hanson, Seth; Daily, Neil J; Wakatsuki, Tetsuro

    2015-05-14

    Continued advancement in pluripotent stem cell culture is closing the gap between bench and bedside for using these cells in regenerative medicine, drug discovery and safety testing. In order to produce stem cell derived biopharmaceutics and cells for tissue engineering and transplantation, a cost-effective cell-manufacturing technology is essential. Maintenance of pluripotency and stable performance of cells in downstream applications (e.g., cell differentiation) over time is paramount to large scale cell production. Yet that can be difficult to achieve especially if cells are cultured manually where the operator can introduce significant variability as well as be prohibitively expensive to scale-up. To enable high-throughput, large-scale stem cell production and remove operator influence novel stem cell culture protocols using a bench-top multi-channel liquid handling robot were developed that require minimal technician involvement or experience. With these protocols human induced pluripotent stem cells (iPSCs) were cultured in feeder-free conditions directly from a frozen stock and maintained in 96-well plates. Depending on cell line and desired scale-up rate, the operator can easily determine when to passage based on a series of images showing the optimal colony densities for splitting. Then the necessary reagents are prepared to perform a colony split to new plates without a centrifugation step. After 20 passages (~3 months), two iPSC lines maintained stable karyotypes, expressed stem cell markers, and differentiated into cardiomyocytes with high efficiency. The system can perform subsequent high-throughput screening of new differentiation protocols or genetic manipulation designed for 96-well plates. This technology will reduce the labor and technical burden to produce large numbers of identical stem cells for a myriad of applications.

  4. Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

    PubMed Central

    Conway, Michael K.; Gerger, Michael J.; Balay, Erin E.; O'Connell, Rachel; Hanson, Seth; Daily, Neil J.; Wakatsuki, Tetsuro

    2015-01-01

    Continued advancement in pluripotent stem cell culture is closing the gap between bench and bedside for using these cells in regenerative medicine, drug discovery and safety testing. In order to produce stem cell derived biopharmaceutics and cells for tissue engineering and transplantation, a cost-effective cell-manufacturing technology is essential. Maintenance of pluripotency and stable performance of cells in downstream applications (e.g., cell differentiation) over time is paramount to large scale cell production. Yet that can be difficult to achieve especially if cells are cultured manually where the operator can introduce significant variability as well as be prohibitively expensive to scale-up. To enable high-throughput, large-scale stem cell production and remove operator influence novel stem cell culture protocols using a bench-top multi-channel liquid handling robot were developed that require minimal technician involvement or experience. With these protocols human induced pluripotent stem cells (iPSCs) were cultured in feeder-free conditions directly from a frozen stock and maintained in 96-well plates. Depending on cell line and desired scale-up rate, the operator can easily determine when to passage based on a series of images showing the optimal colony densities for splitting. Then the necessary reagents are prepared to perform a colony split to new plates without a centrifugation step. After 20 passages (~3 months), two iPSC lines maintained stable karyotypes, expressed stem cell markers, and differentiated into cardiomyocytes with high efficiency. The system can perform subsequent high-throughput screening of new differentiation protocols or genetic manipulation designed for 96-well plates. This technology will reduce the labor and technical burden to produce large numbers of identical stem cells for a myriad of applications. PMID:26068617

  5. Evaluation of drug load and polymer by using a 96-well plate vacuum dry system for amorphous solid dispersion drug delivery.

    PubMed

    Chiang, Po-Chang; Ran, Yingqing; Chou, Kang-Jye; Cui, Yong; Sambrone, Amy; Chan, Connie; Hart, Ryan

    2012-06-01

    It is well recognized that poor dissolution rate and solubility of drug candidates are key limiting factors for oral bioavailability. While numerous technologies have been developed to enhance solubility of the drug candidates, poor water solubility continuously remains a challenge for drug delivery. Among those technologies, amorphous solid dispersions (SD) have been successfully employed to enhance both dissolution rate and solubility of poorly water-soluble drugs. This research reports a high-throughput screening technology developed by utilizing a 96-well plate system to identify optimal drug load and polymer using a solvent casting approach. A minimal amount of drug was required to evaluate optimal drug load in three different polymers with respect to solubility improvement and solid-state stability of the amorphous drug-polymer system. Validation of this method was demonstrated with three marketed drugs as well as with one internal compound. Scale up of the internal compound SD by spray drying further confirmed the validity of this method, and its quality was comparable to a larger scale process. Here, we demonstrate that our system is highly efficient, cost-effective, and robust to evaluate the feasibility of spray drying technology to produce amorphous solid dispersions.

  6. HIGHLY SENSITIVE ASSAY FOR ANTICHOLINESTERASE COMPOUNDS USING 96 WELL PLATE FORMAT

    EPA Science Inventory

    The rapid and sensitive detection of organophosphate insecticides using a 96 well plate format is reported. Several features of this assay make it attractive for development as a laboratory-based or field screening assay. Acetylcholinesterase (AChE) was stabilized in a gelati...

  7. 96-Well plate assays for measuring collagenase activity using (3)H-acetylated collagen.

    PubMed

    Koshy, P J; Rowan, A D; Life, P F; Cawston, T E

    1999-11-15

    We describe two alternative assays for measuring collagenolytic activity using (3)H-acetylated collagen. Both assays have been developed for the 96-well plate format and measure the amount of radiolabeled collagen fragments released into the supernatant from an insoluble (3)H-acetylated collagen fibril preparation. The first method separates digested solubilized fragments from the intact fibril by sedimentation of the undigested collagen by centrifugation. The second method achieves this separation by filtration of the supernatant through the membrane of a 96-well filtration plate which retains the undigested collagen fibril. Both methods give linear dose- and time-dependent responses of collagenase activity > or = 70% of total collagen lysis. In addition, both assays can be simply modified to measure tissue inhibitors of metalloproteinases (TIMPs) inhibitory activity, which is also linear between 20 and 75% of total collagen lysis with the amount of TIMP added.

  8. A 96-well plate assay for CYP4503A induction using cryopreserved human hepatocytes.

    PubMed

    Kamiguchi, Naomi; Aoyama, Eiji; Okuda, Teruaki; Moriwaki, Toshiya

    2010-11-01

    A reliable and practical CYP3A induction assay with cryopreserved human hepatocytes in a 96-well format was developed. Various 96-well plates with different basement membrane were evaluated using prototypical inducers, rifampicin, phenytoin, and carbamazepine. Thin-layer (TL) Matrigel was found to yield the highest basal and induced levels of CYP3A activity as determined by testosterone 6β-hydroxylation. Concentration-dependent CYP3A induction of rifampicin was reproducible with the EC(50) values of 0.36 ± 0.28 μM from four batches of human hepatocytes using the 96-well plate with TL Matrigel. The rank order of induction potency for nine inducers or noninducers at a concentration of 10 μM were well comparable among the multiple donors, by expressing the results as percentage of change compared with the positive control, 10 μM rifampicin. Cotreatment of avasimibe or efavirenz with 10 μM rifampicin was found to reduce CYP3A activities induced by rifampicin at a lower rate than treatment with rifampicin alone, whereas treatment with phenobarbital and carbamazepine had no effect. From a comparison of induced CYP3A activities and gene expression levels, there were compounds that would cause induction of CYP3A4 mRNA but not activity, presumably due to their inhibitory effect on CYP3A activity. The cotreatment assay of test compound with rifampicin allows us to exclude the false-negative results caused by the cytotoxicity and/or the mechanism-based inactivation, when the drug candidate's ability for CYP3A induction is evaluating the enzyme activity. This 96-well plate assay, which is robust, reproducible, and convenient, has demonstrated the paramount applicability to the early drug discovery stage.

  9. Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis.

    PubMed

    Fitzpatrick, Brittany; Schuler, Catherine; Leggett, Robert E; Levin, Robert M

    2012-01-01

    Purpose. Nitrotyrosine was quantitated in rabbit bladder muscle and mucosa using two analytical systems: Western blotting analyses and a 96-well plate quantitative analysis kit. Materials and Methods. Rabbit bladder muscle and mucosa were obtained from control rabbits. For the Western analysis, the samples were loaded into a SDS page gel and then transferred to a PVDF membrane. The optical density was measured using a Kodak Scanner. Using the 96-well plate, the samples and standards were loaded, incubated with primary and secondary antibody, washed and vacuumed with 10x wash buffer three times between each incubation period. Stop buffer was added to the plate and the results were quantified via the plate reader. Results. For both muscle and mucosa tissue, the optical density readings were linear with tissue concentration; the concentration of nitrotyrosine in the mucosa was significantly higher than in the muscle. However, whereas the Western blot analysis is based on relative optical densities, the 96-well plate kit provides a truly quantitative analysis. Discussion. Mucosa tissue displayed a higher density of nitrotyrosine than did detrusor muscle tissue. This may well be due to the significantly higher metabolic activity of the mucosa compared to the muscle.

  10. Urine sample preparation in 96-well filter plates for quantitative clinical proteomics.

    PubMed

    Yu, Yanbao; Suh, Moo-Jin; Sikorski, Patricia; Kwon, Keehwan; Nelson, Karen E; Pieper, Rembert

    2014-06-03

    Urine is an important, noninvasively collected body fluid source for the diagnosis and prognosis of human diseases. Liquid chromatography mass spectrometry (LC-MS) based shotgun proteomics has evolved as a sensitive and informative technique to discover candidate disease biomarkers from urine specimens. Filter-aided sample preparation (FASP) generates peptide samples from protein mixtures of cell lysate or body fluid origin. Here, we describe a FASP method adapted to 96-well filter plates, named 96FASP. Soluble urine concentrates containing ~10 μg of total protein were processed by 96FASP and LC-MS resulting in 700-900 protein identifications at a 1% false discovery rate (FDR). The experimental repeatability, as assessed by label-free quantification and Pearson correlation analysis for shared proteins among replicates, was high (R ≥ 0.97). Application to urinary pellet lysates which is of particular interest in the context of urinary tract infection analysis was also demonstrated. On average, 1700 proteins (±398) were identified in five experiments. In a pilot study using 96FASP for analysis of eight soluble urine samples, we demonstrated that protein profiles of technical replicates invariably clustered; the protein profiles for distinct urine donors were very different from each other. Robust, highly parallel methods to generate peptide mixtures from urine and other body fluids are critical to increase cost-effectiveness in clinical proteomics projects. This 96FASP method has potential to become a gold standard for high-throughput quantitative clinical proteomics.

  11. Urine Sample Preparation in 96-Well Filter Plates for Quantitative Clinical Proteomics

    PubMed Central

    2015-01-01

    Urine is an important, noninvasively collected body fluid source for the diagnosis and prognosis of human diseases. Liquid chromatography mass spectrometry (LC-MS) based shotgun proteomics has evolved as a sensitive and informative technique to discover candidate disease biomarkers from urine specimens. Filter-aided sample preparation (FASP) generates peptide samples from protein mixtures of cell lysate or body fluid origin. Here, we describe a FASP method adapted to 96-well filter plates, named 96FASP. Soluble urine concentrates containing ∼10 μg of total protein were processed by 96FASP and LC-MS resulting in 700–900 protein identifications at a 1% false discovery rate (FDR). The experimental repeatability, as assessed by label-free quantification and Pearson correlation analysis for shared proteins among replicates, was high (R ≥ 0.97). Application to urinary pellet lysates which is of particular interest in the context of urinary tract infection analysis was also demonstrated. On average, 1700 proteins (±398) were identified in five experiments. In a pilot study using 96FASP for analysis of eight soluble urine samples, we demonstrated that protein profiles of technical replicates invariably clustered; the protein profiles for distinct urine donors were very different from each other. Robust, highly parallel methods to generate peptide mixtures from urine and other body fluids are critical to increase cost-effectiveness in clinical proteomics projects. This 96FASP method has potential to become a gold standard for high-throughput quantitative clinical proteomics. PMID:24797144

  12. Development of a novel bead-based 96-well filtration plate competitive immunoassay for the detection of Gentamycin.

    PubMed

    Ho, Tien Yu Jessica; Chan, Chia-Chung; Chan, KinGho; Wang, Yu Chieh; Lin, Jing-Tang; Chang, Cheng-Ming; Chen, Chien-Sheng

    2013-11-15

    We developed a sensitive, simple, inexpensive and rapid bead-based immunoassay platform, composed of liposomal nanovesicle amplification system, Gentamycin sulfate beads and 96-well filtration plates. In the beginning of the assay, Gentamycin sulfate beads, Gentamycin sulfate and Gentamycin specific antibody were incubated in a bottom-sealed 96-well filtration plate. After incubation, washing was done by running washing buffer through the unsealed filtration plate with only gravity and the antibody-Gentamycin bead complexes were retained in the plate. Fluorescent dye-loaded protein G-liposomal nanovesicles were then added to specifically bind to antibodies on the retained beads. After washing unbound nanovesicles, millions of fluorescent dye molecules were released by adding a detergent solution to lyse liposomal nanovesicles. The limit of detection (LOD) of this novel detection platform in TBS and in skim milk were 52.65 ng/mL and 14.16 ng/mL, which are both sufficient for detecting the 200 ng/mL Codex maximum residual level (MRL). The dynamic ranges were both from each of their LODs to 100 μg/mL. The 50% inhibition concentrations (IC50) in TBS and skim milk were 199.66 ng/mL and 360.81 ng/mL, respectively. We also demonstrated the good specificity of this platform by comparing detection results between pure Gentamycin solution and a mixture solution of 6 different antibiotics including Gentamycin in skim milk. The entire assay with 60 samples was conducted within 2h. In sum, this novel biosensing platform not only fulfilled most benefits of magnetic bead-based assays, but also was inexpensive and convenient by replacing the magnetic separation with filtration plate separation.

  13. Ice-Cap: a method for growing Arabidopsis and tomato plants in 96-well plates for high-throughput genotyping.

    PubMed

    Su, Shih-Heng; Clark, Katie A; Gibbs, Nicole M; Bush, Susan M; Krysan, Patrick J

    2011-11-09

    It is becoming common for plant scientists to develop projects that require the genotyping of large numbers of plants. The first step in any genotyping project is to collect a tissue sample from each individual plant. The traditional approach to this task is to sample plants one-at-a-time. If one wishes to genotype hundreds or thousands of individuals, however, using this strategy results in a significant bottleneck in the genotyping pipeline. The Ice-Cap method that we describe here provides a high-throughput solution to this challenge by allowing one scientist to collect tissue from several thousand seedlings in a single day (1,2). This level of throughput is made possible by the fact that tissue is harvested from plants 96-at-a-time, rather than one-at-a-time. The Ice-Cap method provides an integrated platform for performing seedling growth, tissue harvest, and DNA extraction. The basis for Ice-Cap is the growth of seedlings in a stacked pair of 96-well plates. The wells of the upper plate contain plugs of agar growth media on which individual seedlings germinate. The roots grow down through the agar media, exit the upper plate through a hole, and pass into a lower plate containing water. To harvest tissue for DNA extraction, the water in the lower plate containing root tissue is rapidly frozen while the seedlings in the upper plate remain at room temperature. The upper plate is then peeled away from the lower plate, yielding one plate with 96 root tissue samples frozen in ice and one plate with 96 viable seedlings. The technique is named "Ice-Cap" because it uses ice to capture the root tissue. The 96-well plate containing the seedlings can then wrapped in foil and transferred to low temperature. This process suspends further growth of the seedlings, but does not affect their viability. Once genotype analysis has been completed, seedlings with the desired genotype can be transferred from the 96-well plate to soil for further propagation. We have demonstrated

  14. A 96-well screen filter plate for high-throughput biological sample preparation and LC-MS/MS analysis.

    PubMed

    Peng, Sean X; Cousineau, Martin; Juzwin, Stephen J; Ritchie, David M

    2006-01-01

    A novel 96-well screen filter plate (patent pending) has been invented to eliminate a time-consuming and labor-intensive step in preparation of in vivo study samples--to remove blood or plasma clots. These clots plug the pipet tips during a manual or automated sample-transfer step causing inaccurate pipetting or total pipetting failure. Traditionally, these blood and plasma clots are removed by picking them out manually one by one from each sample tube before any sample transfer can be made. This has significantly slowed the sample preparation process and has become a bottleneck for automated high-throughput sample preparation using robotic liquid handlers. Our novel screen filter plate was developed to solve this problem. The 96-well screen filter plate consists of 96 stainless steel wire-mesh screen tubes connected to the 96 openings of a top plate so that the screen filter plate can be readily inserted into a 96-well sample storage plate. Upon insertion, the blood and plasma clots are excluded from entering the screen tube while clear sample solutions flow freely into it. In this way, sample transfer can be easily completed by either manual or automated pipetting methods. In this report, three structurally diverse compounds were selected to evaluate and validate the use of the screen filter plate. The plasma samples of these compounds were transferred and processed in the presence and absence of the screen filter plate and then analyzed by LC-MS/MS methods. Our results showed a good agreement between the samples prepared with and without the screen filter plate, demonstrating the utility and efficiency of this novel device for preparation of blood and plasma samples. The device is simple, easy to use, and reusable. It can be employed for sample preparation of other biological fluids that contain floating particulates or aggregates.

  15. Performance evaluation of 3D polystyrene 96-well plates with human neural stem cells in a calcium assay.

    PubMed

    Lai, Yinzhi; Kisaalita, William S

    2012-08-01

    In this study, we have generated a high-throughput screening (HTS)-compatible 3D cell culture platform by chemically "welding" polystyrene scaffolds into standard 2D polystyrene 96-well plates. The variability of scaffolds was minimized by introducing automation into the fabrication process. The fabricated 3D cell culture plates were compared with several commercially available 3D cell culture platforms with light and scanning electron microscopy. Voltage-gated calcium channel functionality was used to access the Z' factors of all plates, including a 2D standard plate control. It was found that with the No-Wash Fluo-4 calcium assay and neural progenitor cells, all plates display acceptable Z' factors for use in HTS. The plates with "welded" polystyrene scaffolds have several advantages, such as being versatile and economical, and are ready to use off the shelf. These characteristics are especially desired in HTS preclinical drug discovery applications.

  16. Thermodynamic equilibrium solubility measurements in simulated fluids by 96-well plate method in early drug discovery.

    PubMed

    Bharate, Sonali S; Vishwakarma, Ram A

    2015-04-01

    An early prediction of solubility in physiological media (PBS, SGF and SIF) is useful to predict qualitatively bioavailability and absorption of lead candidates. Despite of the availability of multiple solubility estimation methods, none of the reported method involves simplified fixed protocol for diverse set of compounds. Therefore, a simple and medium-throughput solubility estimation protocol is highly desirable during lead optimization stage. The present work introduces a rapid method for assessment of thermodynamic equilibrium solubility of compounds in aqueous media using 96-well microplate. The developed protocol is straightforward to set up and takes advantage of the sensitivity of UV spectroscopy. The compound, in stock solution in methanol, is introduced in microgram quantities into microplate wells followed by drying at an ambient temperature. Microplates were shaken upon addition of test media and the supernatant was analyzed by UV method. A plot of absorbance versus concentration of a sample provides saturation point, which is thermodynamic equilibrium solubility of a sample. The established protocol was validated using a large panel of commercially available drugs and with conventional miniaturized shake flask method (r(2)>0.84). Additionally, the statistically significant QSPR models were established using experimental solubility values of 52 compounds.

  17. A method for high throughput determination of viable bacteria cell counts in 96-well plates

    PubMed Central

    2012-01-01

    Background There are several methods for quantitating bacterial cells, each with advantages and disadvantages. The most common method is bacterial plating, which has the advantage of allowing live cell assessment through colony forming unit (CFU) counts but is not well suited for high throughput screening (HTS). On the other hand, spectrophotometry is adaptable to HTS applications but does not differentiate between dead and living bacteria and has low sensitivity. Results Here, we report a bacterial cell counting method termed Start Growth Time (SGT) that allows rapid and serial quantification of the absolute or relative number of live cells in a bacterial culture in a high throughput manner. We combined the methodology of quantitative polymerase chain reaction (qPCR) calculations with a previously described qualitative method of bacterial growth determination to develop an improved quantitative method. We show that SGT detects only live bacteria and is sensitive enough to differentiate between 40 and 400 cells/mL. SGT is based on the re-growth time required by a growing cell culture to reach a threshold, and the notion that this time is proportional to the number of cells in the initial inoculum. We show several applications of SGT, including assessment of antibiotic effects on cell viability and determination of an antibiotic tolerant subpopulation fraction within a cell population. SGT results do not differ significantly from results obtained by CFU counts. Conclusion SGT is a relatively quick, highly sensitive, reproducible and non-laborious method that can be used in HTS settings to longitudinally assess live cells in bacterial cell cultures. PMID:23148795

  18. A Novel 96well-formatted Micro-gap Plate Enabling Drug Response Profiling on Primary Tumour Samples

    NASA Astrophysics Data System (ADS)

    Ma, Wei-Yuan; Hsiung, Lo-Chang; Wang, Chen-Ho; Chiang, Chi-Ling; Lin, Ching-Hung; Huang, Chiun-Sheng; Wo, Andrew M.

    2015-04-01

    Drug-based treatments are the most widely used interventions for cancer management. Personalized drug response profiling remains inherently challenging with low cell count harvested from tumour sample. We present a 96well-formatted microfluidic plate with built-in micro-gap that preserves up to 99.2% of cells during multiple assay/wash operation and only 9,000 cells needed for a single reagent test (i.e. 1,000 cells per test spot x 3 selected concentration x triplication), enabling drug screening and compatibility with conventional automated workstations. Results with MCF7 and MDA-MB-231 cell lines showed that no statistical significance was found in dose-response between the device and conventional 96-well plate control. Primary tumour samples from breast cancer patients tested in the device also showed good IC50 prediction. With drug screening of primary cancer cells must consider a wide range of scenarios, e.g. suspended/attached cell types and rare/abundant cell availability, the device enables high throughput screening even for suspended cells with low cell count since the signature microfluidic cell-trapping feature ensures cell preservation in a multiple solution exchange protocol.

  19. Mass Spectrometric N-Glycan Analysis of Haptoglobin from Patient Serum Samples Using a 96-Well Plate Format.

    PubMed

    Zhu, Jianhui; Wu, Jing; Yin, Haidi; Marrero, Jorge; Lubman, David M

    2015-11-06

    Alterations in glycosylation of serum glycoproteins can provide unique and highly specific fingerprints of malignancy. Our previous mass spectrometric study revealed that the bifucosylation level of serum haptoglobin was distinctly increased in hepatocellular carcinoma (HCC) patients versus liver cirrhosis of all three major etiologies. We have thus developed a method for the analysis of large numbers of serum samples based on a 96-well plate platform for the evaluation of fucosylation changes of serum haptoglobin between HCC versus cirrhosis. Haptoglobin was isolated from the serum of individual patient samples based on an HPLC column immobilized with antihaptoglobin antibody via hydrazide immobilization chemistry. Only 10 μL of serum was required for glycan extraction and processing for MALDI-QIT mass spectrometry analysis using the 96-well plate format. The bifucosylation degrees of haptoglobin in individuals were calculated using a quantitative glycomics method. The MS data confirmed that the bifucosylated tetra-anntenary glycan was upregulated in HCC samples of all etiologies. This study provides a parallel method for processing glycan content for haptoglobin and evaluating detailed changes in glycan structures for a potentially large cohort of clinical serum samples.

  20. 96-Well Plate Colorimetric Assay for K(sub i) Determination of (plusmn)-2-Benzylsuccinic Acid, an Inhibitor of Carboxypeptidase A

    ERIC Educational Resources Information Center

    Wentland, Mark P.; Raza, Shaan; Yingtong Gao

    2004-01-01

    An appropriate assay to determine the inhibition potency of carboxypeptidase A (CPA) in 96-well format to illustrate how high throughput screening is used in modern drug discovery to identify bioactive molecules is developed. Efforts in developing a colorimetric 96-well plate assay for determination of the K(sub i) for inhibition of CPA by…

  1. Equilibrium drug solubility measurements in 96-well plates reveal similar drug solubilities in phosphate buffer pH 6.8 and human intestinal fluid.

    PubMed

    Heikkilä, Tiina; Karjalainen, Milja; Ojala, Krista; Partola, Kirsi; Lammert, Frank; Augustijns, Patrick; Urtti, Arto; Yliperttula, Marjo; Peltonen, Leena; Hirvonen, Jouni

    2011-02-28

    This study was conducted to develop a high throughput screening (HTS) method for the assessment of equilibrium solubility of drugs. Solid-state compounds were precipitated from methanol in 96-well plates, in order to eliminate the effect of co-solvent. Solubility of twenty model drugs was analyzed in water and aqueous solutions (pH 1.2 and 6.8) in 96-well plates and in shake-flasks (UV detection). The results obtained with the 96-well plate method correlated well (R(2)=0.93) between the shake-flask and 96-well plates over the wide concentration scale of 0.002-169.2mg/ml. Thereafter, the solubility tests in 96-well plates were performed using fasted state human intestinal fluid (HIF) from duodenum of healthy volunteers. The values of solubility were similar in phosphate buffer solution (pH 6.8) and HIF over the solubility range of 10(2)-10(5)μg/ml. The new 96-well plate method is useful for the screening of equilibrium drug solubility during the drug discovery process and it also allows the use of human intestinal fluid in solubility screening.

  2. Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates

    PubMed Central

    Maioli, Vincent; Chennell, George; Sparks, Hugh; Lana, Tobia; Kumar, Sunil; Carling, David; Sardini, Alessandro; Dunsby, Chris

    2016-01-01

    Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM). Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Förster resonance energy transfer (FRET) biosensor is demonstrated in 16 fields of view with image acquisition at 10 minute intervals. As a proof-of-principle, the ssOPM system is also used to acquire a dose response curve with the concentration of glucose in the culture medium being varied across 42 wells of a 96-well plate with the whole acquisition taking 9 min. The 3-D image data enable the FRET ratio to be measured as a function of distance from the surface of the spheroid. Overall, the results demonstrate the capability of the OPM system to measure spatio-temporal changes in FRET ratio in 3-D in multicellular spheroids over time in a multi-well plate format. PMID:27886235

  3. Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates

    NASA Astrophysics Data System (ADS)

    Maioli, Vincent; Chennell, George; Sparks, Hugh; Lana, Tobia; Kumar, Sunil; Carling, David; Sardini, Alessandro; Dunsby, Chris

    2016-11-01

    Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM). Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Förster resonance energy transfer (FRET) biosensor is demonstrated in 16 fields of view with image acquisition at 10 minute intervals. As a proof-of-principle, the ssOPM system is also used to acquire a dose response curve with the concentration of glucose in the culture medium being varied across 42 wells of a 96-well plate with the whole acquisition taking 9 min. The 3-D image data enable the FRET ratio to be measured as a function of distance from the surface of the spheroid. Overall, the results demonstrate the capability of the OPM system to measure spatio-temporal changes in FRET ratio in 3-D in multicellular spheroids over time in a multi-well plate format.

  4. Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates.

    PubMed

    Maioli, Vincent; Chennell, George; Sparks, Hugh; Lana, Tobia; Kumar, Sunil; Carling, David; Sardini, Alessandro; Dunsby, Chris

    2016-11-25

    Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM). Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Förster resonance energy transfer (FRET) biosensor is demonstrated in 16 fields of view with image acquisition at 10 minute intervals. As a proof-of-principle, the ssOPM system is also used to acquire a dose response curve with the concentration of glucose in the culture medium being varied across 42 wells of a 96-well plate with the whole acquisition taking 9 min. The 3-D image data enable the FRET ratio to be measured as a function of distance from the surface of the spheroid. Overall, the results demonstrate the capability of the OPM system to measure spatio-temporal changes in FRET ratio in 3-D in multicellular spheroids over time in a multi-well plate format.

  5. A new approach to the application of solid phase extraction disks with LC-MS/MS for the analysis of drugs on a 96-well plate format.

    PubMed

    Cudjoe, Erasmus; Pawliszyn, Janusz

    2009-11-01

    A new 96-well disk solid phase extraction sample preparation technique which does not involve vacuum pumps integrated with liquid chromatographic tandem mass spectrometric (LC-MS/MS) was developed for high throughput determination of benzodiazepines (nordiazepam, diazepam, lorazepam and oxazepam). In addition, the method completely allows the re-use of the SPE disk membranes for subsequent analyses after re-conditioning. The method utilizes a robotic autosampler for parallel extractions in a 96-well plate format. Results have been presented for independent extractions from three matrices; phosphate buffer solution, urine, and plasma. Factors affecting data reproducibility, extraction kinetics, sample throughput, and reliability of the system were investigated and optimized. A total time required per sample was 0.94 min using 96-well format. Method reproducibility was < or =9% relative standard deviation for all three matrices. Limits of detection and quantitation recorded were respectively in the range 0.02-0.15 and 0.2-2.0 ng/mL with linearity ranging from 0.2 to 500 ng/mL for all matrices.

  6. 96-well plate-to-plate gravity fluorous solid-phase extraction (F-SPE) for solution-phase library purification.

    PubMed

    Zhang, Wei; Lu, Yimin

    2007-01-01

    Large particle size (125_210 microm) fluorous silica gel bonded with a -SiCH2CH2C8F17 stationary phase has been employed for gravity-driven fluorous solid-phase extraction (F-SPE) on two types of 96-well plates. A 1 or 0.75 g portion of fluorous silica is packed to each well of the 3.5-mL Ex-Blok and the 2.2-mL deep-well filtration plates, respectively. Up to 50 mg of reaction mixture is loaded and then eluted with a fluorophobic solvent (DMSO, DMF, or 85:15 DMF-H2O). Products collected in 96-well receiving plates are directly concentrated on a GeneVac vacuum centrifuge. This simple and highly efficient plate-to-plate F-SPE technique has been demonstrated in the purification of four 96-compound libraries produced by scavenging reactions with 1-(perfluoroctyl)propyl isatoic anhydride (F-IA), amide coupling reactions with 2-chloro-4,6-bis[(perfluorooctyl)propyloxy]-1,3,5-triazine (F-CDMT) or 2,4-dichloro-6-(perfluorooctyl)propyloxy-1,3,5-triazine (F-DCT), and Mitsunobu reactions with fluorous diethyl azodicarboxylate (F-DEAD) and triphenylphosphine (F-TPP). Approximately 80% of products in each library have greater than 85% purity after F-SPE without conducting chromatography.

  7. Assessment of a 96-Well Plate Assay of Quantitative Drug Susceptibility Testing for Mycobacterium Tuberculosis Complex in China

    PubMed Central

    Xia, Hui; Zheng, Yang; Zhao, Bing; van den Hof, Susan; Cobelens, Frank; Zhao, YanLin

    2017-01-01

    Objective To evaluate the performance of the Sensitire MYCOTB MIC Plate (MYCOTB) which could measure the twelve anti-tuberculosis drugs susceptibility on one 96-wells plate. Methods A total of 140 MDR-TB strains and 60 non-MDR strains were sub-cultured and 193 strains were finally tested for drug resistance using MYCOTB and agar proportion method (APM) and another 7 strains failed of subculture. The drugs included ofloxacin (Ofx), moxifloxacin (Mfx), rifampin (RFP), amikacin (Am), rifabutin (Rfb), para-aminosalicylic acid (PAS), ethionamide (Eth), isoniazid (INH), kanamycin (Km), ethambutol (EMB), streptomycin (Sm), and cycloserine(Cs). The categorical agreement, conditional agreement, sensitivity and specificity of MYCOTB were assessed in comparison with APM. For strains with inconsistent results between MYCOTB and APM, the drug resistance related gene fragments were amplified and sequenced: gyrA for Ofx and Mfx; rpoB for RFP and Rfb; embB for EMB; rpsl for Sm; katG and the promoter region of inhA for INH, ethA and the promoter region of inhA for Eth. The sequence results were compared with results of MYCOTB and APM to analyze the consistency between sequence results and MYCOTB or APM. Results The categorical agreement between two methods for each drug ranged from 88.6% to 100%. It was the lowest for INH (88.6%). The sensitivity and specificity of MYCOTB ranged from 71.4% to 100% and 84.3% to 100%, respectively. The sensitivity was lowest for Cs(71.4%), EMB at 10μg/ml (80.0%) and INH at 10.0μg/ml (84.6%). The specificity was lowest for Rfb (84.3%). Overall discordance between the two phenotypic methods was observed for 96 strains, of which 63 (65.6%) were found susceptible with APM and resistant with MYCOTB and the remaining 33(34.4%) strains were resistant by APM and susceptible with MYCOTB. 34/52 (65.4%) sequenced APM susceptible and MYCOTB resistant(APM-S/MYCOTB-R) strains had mutations or insertions in the amplified regions. 20/30 (66.7%) sequenced APM

  8. High-throughput determination of fudosteine in human plasma by liquid chromatography-tandem mass spectrometry, following protein precipitation in the 96-well plate format.

    PubMed

    Wen, Jun; Wu, Yiwen; Zhang, Linli; Qi, Yunpeng; Fan, Guorong; Wu, Yutian; Li, Zhen

    2008-05-01

    A 96-well protein precipitation, liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and fully validated for the determination of fudosteine in human plasma. After protein precipitation of the plasma samples (50 microL) by the methanol (150 microL) containing the internal standard (IS), erdosteine, the 96-well plate was vortexed for 5 min and centrifuged for 15 min. The 100 microL supernatant and 100 microL mobile phase were added to another plate and mixed and then the mixture was directly injected into the LC-MS/MS system in the negative ionization mode. The separation was performed on a XB-CN column for 3.0 min per sample using an eluent of methanol-water (60:40, v/v) containing 0.005% formic acid. Multiple reaction monitoring (MRM) using the precursor-product ion transitions m/z 178-->91 and m/z 284-->91 was performed to quantify fudosteine and erdosteine, respectively. The method was sensitive with a lower limit of quantification (LLOQ) of 0.02 microg mL(-1), with good linearity (r>0.999) over the linear range of 0.02-10 microg mL(-1). The within- and between-run precision was less than 5.5% and accuracy ranged from 94.2 to 106.7% for quality control (QC) samples at three concentrations of 0.05, 1 and 8 microg mL(-1). The method was employed in the clinical pharmacokinetic study of fudosteine formulation product after oral administration to healthy volunteers.

  9. Quantum dot immunoassays in renewable surface column and 96-well plate formats for the fluorescence detection of Botulinum neurotoxin using high-affinity antibodies

    SciTech Connect

    Warner, Marvin G.; Grate, Jay W.; Tyler, Abby J.; Ozanich, Richard M.; Miller, Keith D.; Lou, Jianlong; Marks, James D.; Bruckner-Lea, Cindy J.

    2009-09-01

    A fluorescence sandwich immunoassay using high affinity antibodies and quantum dot (QD) reporters has been developed for detection of botulinum toxin serotype A (BoNT/A). For the development of the assay, a nontoxic recombinant fragment of the holotoxin (BoNT/A-HC-fragment) has been used as a structurally valid simulant for the full toxin molecule. The antibodies used, AR4 and RAZ1, bind to nonoverlapping epitopes present on both the full toxin and on the recombinant fragment. In one format, the immunoassay is carried out in a 96-well plate with detection in a standard plate reader. Detection down to 31 pM of the BoNT/Hc-fragment was demonstrated with a total incubation time of 3 hours, using AR4 as the capture antibody and QD-coupled RAZ1 as the reporter. In a second format, the AR4 capture antibody was coupled to Sepharose beads, and the immunochemical reactions were carried out in microcentrifuge tubes with an incubation time of 1 hour. These beads were subsequently captured and concentrated in a rotating rod “renewable surface” flow cell as part of a sequential injection fluidic system. This flow cell was equipped with a fiber optic system for fluorescence measurements. In PBS buffer solution matrix, the BoNT/A-HC-fragment was detected to concentrations as low as 5 pM using the fluidic measurement approach.

  10. 96-Well Polycarbonate-Based Microfluidic Titer Plate for High-Throughput Purification of DNA and RNA

    PubMed Central

    Witek, Małgorzata A.; Hupert, Mateusz L.; Park, Daniel S.-W.; Fears, Kirby; Murphy, Michael C.; Soper, Steven A.

    2008-01-01

    We report a simple and effective method for the high-throughput purification of a variety of nucleic acids (NAs) from whole cell lysates or whole blood using a photactivated polycarbonate solid-phase reversible immobilization (PPC-SPRI) microfluidic chip. High-throughput operation was achieved by placing 96 purification beds, each containing an array of 3800 20 µm diameter posts, on a single 3″ × 5″ polycarbonate (PC) wafer fabricated by hot embossing. All beds were interconnected through a common microfluidic network that permitted parallel access through the use of a vacuum and syringe pump for delivery of immobilization buffer (IB) and effluent. The PPC-SPRI purification was accomplished by condensation of NAs onto a UV-modified PC surface in the presence of the IB comprised of polyethylene glycol, NaCl, and ethanol with a composition dependent on the length of the NAs to be isolated and the identity of the sample matrix. The performance of the device was validated by quantification of the recovered material following PCR (for DNA) or RT-PCR (for RNA). The extraction bed load capacity of NAs was 206 ± 93 ng for gDNA and 165 ± 81 ng for TRNA from Escherichia coli. Plate-to-plate variability was found to be 35 ± 10%. The purification process was fast (>30 min) and easy to automate, and the low cost of wafer fabrication makes it appropriate for single-use applications. PMID:18355091

  11. High-throughput 96-well solvent mediated sonic blending synthesis and on-plate solid/solution stability characterization of pharmaceutical cocrystals.

    PubMed

    Luu, Van; Jona, Janan; Stanton, Mary K; Peterson, Matthew L; Morrison, Henry G; Nagapudi, Karthik; Tan, Helming

    2013-01-30

    A 96-well high-throughput cocrystal screening workflow has been developed consisting of solvent-mediated sonic blending synthesis and on-plate solid/solution stability characterization by XRPD. A strategy of cocrystallization screening in selected blend solvents including water mixtures is proposed to not only manipulate solubility of the cocrystal components but also differentiate physical stability of the cocrystal products. Caffeine-oxalic acid and theophylline-oxalic acid cocrystals were prepared and evaluated in relation to saturation levels of the cocrystal components and stability of the cocrystal products in anhydrous and hydrous solvents. AMG 517 was screened with a number of coformers, and solid/solution stability of the resulting cocrystals on the 96-well plate was investigated. A stability trend was observed and confirmed that cocrystals comprised of lower aqueous solubility coformers tended to be more stable in water. Furthermore, cocrystals which could be isolated under hydrous solvent blending condition exhibited superior physical stability to those which could only be obtained under anhydrous condition. This integrated HTS workflow provides an efficient route in an API-sparing approach to screen and identify cocrystal candidates with proper solubility and solid/solution stability properties.

  12. Development of cystic embryoid bodies with visceral yolk-sac-like structures from mouse embryonic stem cells using low-adherence 96-well plate.

    PubMed

    Yasuda, Emiko; Seki, Yuji; Higuchi, Takatoshi; Nakashima, Fumio; Noda, Tomozumi; Kurosawa, Hiroshi

    2009-04-01

    Cystic embryoid bodies with visceral yolk-sac-like structure (cystic EB-Vs) are used as a model for the study of early extraembryonic tissue formation containing visceral endoderm-like derivatives. In this study, we optimized the cell density of embryonic stem (ES) cells for developing cystic EB-Vs in a low-adherence 96-well plate. When ES cells were seeded at a density of 4000 cells/well, the cystic EB-Vs were most efficiently developed from ES cells via forming multicellular spherical aggregates called embryoid bodies (EBs). The suspension culture in the low-adherence plate was preferable for developing EBs into cystic EB-Vs rather than the attachment culture in the plate coated with 0.1% gelatin. The seeding cell density of 4000 cells/well was always superior to 1000 cells/well in the efficiency of cystic EB-V development. Because the high-cell density culture generally raises the limitation of oxygen and nutrient supplies, we investigated the effects of low-oxygen and low-nutrient conditions on the development of cystic EB-Vs. It was found that low oxygen tension was not a factor for promoting the development of cystic EB-Vs. It was suggested that a low-nutrient medium is preferred for developing cystic EB-Vs rather than a sufficient-nutrient medium. In conclusion, the suspension culture in the low-adherence 96-well plate seeded with 4000 ES cells/well was optimum for developing cystic EB-Vs. The low-nutrient condition may be one of the factors for promoting the development of cystic EB-Vs.

  13. A high-throughput approach for the determination of pesticide residues in cucumber samples using solid-phase microextraction on 96-well plate.

    PubMed

    Bagheri, Habib; Es'haghi, Ali; Es-haghi, Ali; Mesbahi, Noushin

    2012-08-31

    A high-throughput solid-phase microextraction (SPME) on 96-well plate together with gas chromatography-mass spectrometry (GC-MS) was developed for the determination of some selected pesticides in cucumber samples. Pieces with the length of 1.0 cm of silicon tubing were precisely prepared and then coated on the end part of stainless steel wires. The prepared fibers were positioned in a home-made polytetrafluoroethylene (PTFE)-based constructed ninety-six holes block to have the possibility of simultaneous immersion of the SPME fibers into the center of individual wells. Pesticides such as diazinon, penconazol, tebuconazol, bitertanol, malathion, phosalone and chlorpyrifos-methyl were selected for their highly application in cucumber field. The performances of the SPME fibers, such as intra and inter-fibers reproducibility, were evaluated and the results showed a good similarity in extraction yields. A volume of 1 mL of the aquatic supernatant of the cucumber samples was transferred into the 96-well plate and the array of SPME fibers was applied for the extraction of the selected pesticides. The important parameters influencing the whole extraction process including, organic solvent percent, salt addition, dilution factor, stirring rate and extraction time were optimized. The inter- and intra-day RSD% were found to be less than 15.4%. Limits of detection (LOD) and limits of quantification (LOQ) were below 60 and 180 μg kg(-1), respectively. The coefficient of determination was satisfactory (r(2)>0.99) for all the studied analytes. The developed method was successfully applied to the monitoring of several samples gathered from local markets.

  14. A Quantitative High-Throughput 96-well plate Fluorescence Assay for Mechanism-Based Inactivators of Cytochromes P450 Exemplified using CYP2B6

    PubMed Central

    Kenaan, Cesar; Zhang, Haoming; Hollenberg, Paul F.

    2010-01-01

    Mechanism-based inactivators such as bergamottin are useful chemical tools for identifying the roles of specific active-site amino acid residues in the reactions catalyzed by the cytochromes P450 (CYPs or P450s) that are responsible for the metabolism of a wide variety of drugs and endogenous substrates. In clinical settings mechanism-based inactivation of P450s involved in xenobiotic metabolism has the potential to lead to adverse drug-drug interactions and assays to identify and characterize drug candidates as P450 inactivators are important in drug discovery and development. Here we present a quantitative high-throughput protocol for investigating cytochrome P450 mechanism-based inactivators using the example of CYP2B6 and bergamottin to illustrate the finer points of this protocol. This protocol details the adaptation of a 7-ethoxytrifluoromethyl coumarin (7-EFC) O-deethylation fluorescence activity assay to a 96-well microtiter plate format and uses a plate-reader to detect the fluorescence of the product. Compared to previous methods, this protocol requires less P450 and takes significantly less time while greatly increasing throughput. The protocol as written takes approximately two hours to complete. The principles and procedures outlined in this protocol can be easily adapted to other inactivators, P450 isoforms, substrates and plate-readers. PMID:20885377

  15. Induction of apoptosis by intracellular potassium ion depletion: using the fluorescent dye PBFI in a 96-well plate method in cultured lung cancer cells.

    PubMed

    Andersson, B; Janson, V; Behnam-Motlagh, P; Henriksson, R; Grankvist, K

    2006-09-01

    Depletion of intracellular potassium ions (K+) is necessary for cells to shrink, activate caspases and induce DNA fragmentation, events which are features of apoptosis. Here we describe a 96-well plate method using the cell permeable form of K+ binding benzofuran isophtalate (PBFI-AM) to measure intracellular K+ content in relation to untreated control. Cultured human pulmonary mesothelioma cells (P31) and small-cell lung cancer cells (U1690) were treated with K+ flux modulators in order to deprive the cells of intracellular K+. The combination of K+ influx inhibition with 10 micromol/L bumetanide plus 10 micromol/L ouabain and K+ efflux stimulation with 3 mg/L amphotericin B or 5 micromol/L nigericin efficiently reduced the intracellular K+ content after 3 h. Manipulation of K+ fluxes with subsequent intracellular K+ depletion induced apoptosis of lung cancer cells, as detected by caspase-3 activity after 3 h K+ depletion followed by 24 h proliferation and TUNEL positive staining after 48 h proliferation. We concluded that the PBFI-AM assay was a useful tool to determine intracellular K+ content in relation to untreated control, and that intracellular K+ depletion of lung cancer cells by clinically used drugs of relevant concentrations induced apoptosis. These findings may lead to novel therapeutic strategies in the treatment of lung cancer.

  16. Quantitative analysis of simvastatin and its beta-hydroxy acid in human plasma using automated liquid-liquid extraction based on 96-well plate format and liquid chromatography-tandem mass spectrometry.

    PubMed

    Zhang, Nanyan; Yang, Amy; Rogers, John Douglas; Zhao, Jamie J

    2004-01-27

    An assay based on automated liquid-liquid extraction (LLE) and liquid chromatography-tandem mass spectrometry (LC/MS/MS) has been developed and validated for the quantitative analysis of simvastatin (SV) and its beta-hydroxy acid (SVA) in human plasma. A Packard MultiProbe II workstation was used to convert human plasma samples collected following administration of simvastatin and quality control (QC) samples from individual tubes into 96-well plate format. The workstation was also used to prepare calibration standards and spike internal standards. A Tomtec Quadra 96-channel liquid handling workstation was used to perform LLE based on 96-well plates including adding solvents, separating organic from aqueous layer and reconstitution. SV and SVA were separated through a Kromasil C18 column (50 mm x 2 mm i.d., 5 microm) and detected by tandem mass spectrometry with a TurboIonspray interface. Stable isotope-labeled SV and SVA, 13CD(3)-SV and 13 CD(3)-SVA, were used as the internal standards for SV and SVA, respectively. The automated procedures reduced the overall analytical time (96 samples) to 1/3 of that of manual LLE. Most importantly, an analyst spent only a fraction of time on the 96-well LLE. A limit of quantitation of 50 pg/ml was achieved for both SV and SVA. The interconversion between SV and SVA during the 96-well LLE was found to be negligible. The assay showed very good reproducibility, with intra- and inter-assay precision (%R.S.D.) of less than 7.5%, and accuracy of 98.7-102.3% of nominal values for both analytes. By using this method, sample throughput should be enhanced at least three-fold compared to that of the manual procedure.

  17. Development and application of a high-throughput sample cleanup process based on 96-well plate for simultaneous determination of 16 steroids in biological matrices using liquid chromatography-triple quadrupole mass spectrometry.

    PubMed

    Luo, Guanzhong; Li, Youxin; Bao, James J

    2016-02-01

    A novel high-throughput sample pretreatment system was developed by the integration of protein precipitation (PP), phospholipid removal (PPR), and hollow fiber liquid-phase microextraction (HF-LPME) into two simple 96-well plates and a matching 96-grid lid. With this system, 16 steroids were separated from biological matrices of plasma, milk, and urine and analyzed by liquid chromatography-triple quadrupole mass spectrometry. In the tandem sample cleanup process, the prepositive PP and PPR step preliminarily removed some of the interferences from the biological matrices. The following HF-LPME step kept the residual interference out of the hollow fiber and enriched the steroids in the hollow fiber to achieve high sensitivity. By a series of method optimizations, acetonitrile was chosen as the crash solvent for PP and PPR. A mixture of octanol and toluene (1:1 v/v) was used as the acceptor phase for HF-LPME. The extraction was conducted at 80 rpm for 50 min in a donor phase containing 1 mL 20% sodium chloride at 25 °C. Under these conditions, the limits of detection for the 16 steroids were 3.6-300.0 pg(.)mL(-1) in plasma, 3.0-270.0 pg·mL(-1) in milk, and 2.2-210.0 pg(.)mL(-1) in urine. The recoveries of the 16 steroids were 81.9-97.9% in plasma (relative standard deviation 1.0-8.0%), 80.6-97.7% in milk (relative standard deviation 0.8-5.4%), and 87.3-98.7% in urine (relative standard deviation 1.0-4.9%). Further, the integrated 96-well platform of PP, PPR, and HF-LPME enabled us to run this assay in an automatic and high-throughput fashion. The reliability of the method was further corroborated by evaluation of its applicability in plasma and urine samples from volunteers and fresh bovine milk from local dairy enterprises.

  18. Comparison of turbulent-flow chromatography with automated solid-phase extraction in 96-well plates and liquid-liquid extraction used as plasma sample preparation techniques for liquid chromatography-tandem mass spectrometry.

    PubMed

    Zimmer, D; Pickard, V; Czembor, W; Müller, C

    1999-08-27

    Turbulent flow chromatography (TFC) combined with the high selectivity and sensitivity of tandem mass spectrometry (MS-MS) is a new technique for the fast direct analysis of drugs from crude plasma. TFC in the 96-well plate format reduces significantly the time required for sample clean-up in the laboratory. For example, for 100 samples the workload for a technician is reduced from about 8 h by a manual liquid-liquid extraction (LLE) assay to about 1 h in the case of TFC. Sample clean-up and analysis are performed on-line on the same column. Similar chromatographic performance and validation results were achieved using HTLC Turbo-C18 columns (Cohesive Technologies) and Oasis HLB extraction columns (Waters). One 96-well plate with 96 plasma samples is analyzed within 5.25 h, corresponding to 3.3 min per sample. Compared to this LLE and analysis of 96 samples takes about 16 h. Two structurally different and highly protein bound compounds, drug A and drug B, were analyzed under identical TFC conditions and the assays were fully validated for the application to toxicokinetics studies (compliant with Good Laboratory Practices-GLP). The limit of quantitation was 1.00 microg/l and the linear working range covered three orders of magnitude for both drugs. In the case of drug A the quality of analysis by TFC was similar to the reference LLE assay and slightly better than automated solid-phase extraction in 96-well plates. The accuracy was -3.1 to 6.7% and the precision was 3.1 to 6.8% in the case of drug A determined for dog plasma by TFC-MS-MS. For drug B the accuracy was -3.7 to 3.5% and the precision was 1.6 to 5.4% for rat plasma, which is even slightly better than what was achieved with the validated protein precipitation assay.

  19. High-throughput sample preparation for the quantitation of acephate, methamidophos, omethoate, dimethoate, ethylenethiourea, and propylenethiourea in human urine using 96-well-plate automated extraction and high-performance liquid chromatography-tandem mass spectrometry.

    PubMed

    Jayatilaka, Nayana K; Angela Montesano, M; Whitehead, Ralph D; Schloth, Sara J; Needham, Larry L; Barr, Dana Boyd

    2011-07-01

    Acephate, methamidophos, o-methoate, and dimethoate are organophosphorus pesticides, and ethylenethiouria and propylenethiourea are two metabolites from the bisdithiocarbamate fungicide family. They are some of the most widely used pesticides and fungicides in agriculture both domestically and abroad. The existing high-performance liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) method for the measurement of these compounds in human urine was improved by using a 96-well plate format sample preparation; the use of HPLC-MS/MS was comparable with a concentration range of 0.125 to 50 ng/ml. Deuterium-labeled acephate, ethylenethiouria, and methamidophos were used as internal standards. The sample preparation procedure, in the 96-well format with a 0.8-ml urine sample size, uses lyophilization of samples, followed by extraction with dichloromethane. The analytes were chromatographed on a Zorbax SB-C3 (4.6 × 150 mm, 5.0-μm) column with gradient elution by using 0.1% formic acid in aqueous solution (solvent A) and 0.1% formic acid in methanol (solvent B) mobile phase at a flow rate of 1 ml/min. Quantitative analysis was performed by atmospheric pressure chemical ionization source in positive ion mode using multiple-reaction monitoring of the precursor-to-product ion pairs for the analytes on a TSQ Quantum Ultra HPLC-MS/MS. Repeated analyses of urine samples spiked with high (15 ng/ml), medium (5 ng/ml), and low (1 ng/ml) concentrations of the analytes gave relative SDs of <13%. The limits of detection were in the range of 0.004-0.01 ng/ml. The method also has high accuracy, high precision, and excellent extraction recovery. Furthermore, the improved sample preparation method decreased the cost and labor required while effectively doubling the analytic throughput with minimal matrix effect.

  20. Automated liquid-liquid extraction based on 96-well plate format in conjunction with ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for the quantitation of methoxsalen in human plasma.

    PubMed

    Yadav, Manish; Contractor, Pritesh; Upadhyay, Vivek; Gupta, Ajay; Guttikar, Swati; Singhal, Puran; Goswami, Sailendra; Shrivastav, Pranav S

    2008-09-01

    A sensitive, specific and high throughput bioanalytical method using automated sample processing via 96-well plate liquid-liquid extraction and ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) has been developed for the determination of methoxsalen in human plasma. Plasma samples with ketoconazole as internal standard (IS) were prepared by employing 0.2 mL human plasma in ethyl acetate:dichloromethane (80:20, v/v). The chromatographic separation was achieved on a Waters Acquity UPLC BEH C18 column using isocratic mobile phase, consisting of 10 mM ammonium formate and acetonitrile (60:40, v/v), at a flow rate of 0.5 mL/min. The linear dynamic range was established over the concentration range 1.1-213.1 ng/mL for methoxsalen. The method was rugged and rapid with a total run time of 1.5 min. It was successfully applied to a pivotal bioequivalence study in 12 healthy human subjects after oral administration of 10 mg extended release methoxsalen formulation under fasting condition.

  1. 96-well electroporation method for transfection of mammalian central neurons.

    PubMed

    Buchser, William J; Pardinas, Jose R; Shi, Yan; Bixby, John L; Lemmon, Vance P

    2006-11-01

    Manipulating gene expression in primary neurons has been a goal for many scientists for over 20 years. Vertebrate central nervous system neurons are classically difficult to transfect. Most lipid reagents are inefficient and toxic to the cells, and time-consuming methods such as viral infections are often required to obtain better efficiencies. We have developed an efficient method for the transfection of cerebellar granule neurons and hippocampal neurons with standard plasmid vectors. Using 96-well electroporation plates, square-wave pulses can introduce 96 different plasmids into neurons in a single step. The procedure results in greater than 20% transfection efficiencies and requires only simple solutions of nominal cost. In addition to enabling the rapid optimization of experimental protocols with multiple parameters, this procedure enables the use of high content screening methods to characterize neuronal phenotypes.

  2. 96-Well electroporation method for transfection of mammalian central neurons

    PubMed Central

    Buchser, William J.; Pardinas, Jose R.; Shi, Yan; Bixby, John L.; Lemmon, Vance P.

    2008-01-01

    Manipulating gene expression in primary neurons has been a goal for many scientists for over 20 years. Vertebrate central nervous system neurons are classically difficult to transfect. Most lipid reagents are inefficient and toxic to the cells, and time-consuming methods such as viral infections are often required to obtain better efficiencies. We have developed an efficient method for the transfection of cerebellar granule neurons and hippocampal neurons with standard plasmid vectors. Using 96-well electroporation plates, square-wave pulses can introduce 96 different plasmids into neurons in a single step. The procedure results in greater than 20% transfection efficiencies and requires only simple solutions of nominal cost. In addition to enabling the rapid optimization of experimental protocols with multiple parameters, this procedure enables the use of high content screening methods to characterize neuronal phenotypes. PMID:17140120

  3. An improved 96-well turbidity assay for T4 lysozyme activity

    PubMed Central

    Toro, Tasha B.; Nguyen, Thao P.; Watt, Terry J.

    2015-01-01

    T4 lysozyme (T4L) is an important model system for investigating the relationship between protein structure and function. Despite being extensively studied, a reliable, quantitative activity assay for T4L has not been developed. Here, we present an improved T4L turbidity assay as well as an affinity-based T4L expression and purification protocol. This assay is designed for 96-well format and utilizes conditions amenable for both T4L and other lysozymes. This protocol enables easy, efficient, and quantitative characterization of T4L variants and allows comparison between different lysozymes. Our method: • Is applicable for all lysozymes, with enhanced sensitivity for T4 lysozyme compared to other 96-well plate turbidity assays; • Utilizes standardized conditions for comparing T4 lysozyme variants and other lysozymes; and • Incorporates a simplified expression and purification protocol for T4 lysozyme. PMID:26150996

  4. Protocol: high throughput silica-based purification of RNA from Arabidopsis seedlings in a 96-well format

    PubMed Central

    2011-01-01

    The increasing popularity of systems-based approaches to plant research has resulted in a demand for high throughput (HTP) methods to be developed. RNA extraction from multiple samples in an experiment is a significant bottleneck in performing systems-level genomic studies. Therefore we have established a high throughput method of RNA extraction from Arabidopsis thaliana to facilitate gene expression studies in this widely used plant model. We present optimised manual and automated protocols for the extraction of total RNA from 9-day-old Arabidopsis seedlings in a 96 well plate format using silica membrane-based methodology. Consistent and reproducible yields of high quality RNA are isolated averaging 8.9 μg total RNA per sample (~20 mg plant tissue). The purified RNA is suitable for subsequent qPCR analysis of the expression of over 500 genes in triplicate from each sample. Using the automated procedure, 192 samples (2 × 96 well plates) can easily be fully processed (samples homogenised, RNA purified and quantified) in less than half a day. Additionally we demonstrate that plant samples can be stored in RNAlater at -20°C (but not 4°C) for 10 months prior to extraction with no significant effect on RNA yield or quality. Additionally, disrupted samples can be stored in the lysis buffer at -20°C for at least 6 months prior to completion of the extraction procedure providing a flexible sampling and storage scheme to facilitate complex time series experiments. PMID:22136293

  5. Stretch Injury of Human Induced Pluripotent Stem Cell Derived Neurons in a 96 Well Format

    PubMed Central

    Sherman, Sydney A.; Phillips, Jack K.; Costa, J. Tighe; Cho, Frances S.; Oungoulian, Sevan R.; Finan, John D.

    2016-01-01

    Traumatic brain injury (TBI) is a major cause of mortality and morbidity with limited therapeutic options. Traumatic axonal injury (TAI) is an important component of TBI pathology. It is difficult to reproduce TAI in animal models of closed head injury, but in vitro stretch injury models reproduce clinical TAI pathology. Existing in vitro models employ primary rodent neurons or human cancer cell line cells in low throughput formats. This in vitro neuronal stretch injury model employs human induced pluripotent stem cell-derived neurons (hiPSCNs) in a 96 well format. Silicone membranes were attached to 96 well plate tops to create stretchable, culture substrates. A custom-built device was designed and validated to apply repeatable, biofidelic strains and strain rates to these plates. A high content approach was used to measure injury in a hypothesis-free manner. These measurements are shown to provide a sensitive, dose-dependent, multi-modal description of the response to mechanical insult. hiPSCNs transition from healthy to injured phenotype at approximately 35% Lagrangian strain. Continued development of this model may create novel opportunities for drug discovery and exploration of the role of human genotype in TAI pathology. PMID:27671211

  6. High throughput preparation of fly genomic DNA in 96-well format using a paint-shaker.

    PubMed

    Lang, Michael; Nagy, Olga; Lang, Claus; Orgogozo, Virginie

    2015-01-01

    Sample homogenization is an essential step for genomic DNA extraction, with multiple downstream applications in Molecular Biology. Genotyping hundreds or thousands of samples requires an automation of this homogenization step, and high throughput homogenizer equipment currently costs 7000 euros or more. We present an apparatus for homogenization of individual Drosophila adult flies in 96-well micro-titer dishes, which was built from a small portable paint-shaker (F5 portable paint-shaker, Ushake). Single flies are disrupted in each well that contains extraction buffer and a 4-mm metal ball. Our apparatus can hold up to five 96-well micro-titer plates. Construction of the homogenizer apparatus takes about 3-4 days, and all equipment can be obtained from a home improvement store. The total material cost is approximately 700 euros including the paint-shaker. We tested the performance of our apparatus using the ZR-96 Quick-gDNA™ kit (Zymo Research) homogenization buffer and achieved nearly complete tissue homogenization after 15 minutes of shaking. PCR tests did not detect any cross contamination between samples of neighboring wells. We obtained on average 138 ng of genomic DNA per fly, and DNA quality was adequate for standard PCR applications. In principle, our tissue homogenizer can be used for isolation of DNA suitable for library production and high throughput genotyping by Multiplexed Shotgun Genotyping (MSG), as well as RNA isolation from single flies. The sample adapter can also hold and shake other items, such as centrifuge tubes (15-50 mL) or small bottles.

  7. High throughput preparation of fly genomic DNA in 96-well format using a paint-shaker

    PubMed Central

    Lang, Michael; Nagy, Olga; Lang, Claus; Orgogozo, Virginie

    2015-01-01

    Sample homogenization is an essential step for genomic DNA extraction, with multiple downstream applications in Molecular Biology. Genotyping hundreds or thousands of samples requires an automation of this homogenization step, and high throughput homogenizer equipment currently costs 7000 euros or more. We present an apparatus for homogenization of individual Drosophila adult flies in 96-well micro-titer dishes, which was built from a small portable paint-shaker (F5 portable paint-shaker, Ushake). Single flies are disrupted in each well that contains extraction buffer and a 4-mm metal ball. Our apparatus can hold up to five 96-well micro-titer plates. Construction of the homogenizer apparatus takes about 3–4 days, and all equipment can be obtained from a home improvement store. The total material cost is approximately 700 euros including the paint-shaker. We tested the performance of our apparatus using the ZR-96 Quick-gDNA™ kit (Zymo Research) homogenization buffer and achieved nearly complete tissue homogenization after 15 minutes of shaking. PCR tests did not detect any cross contamination between samples of neighboring wells. We obtained on average 138 ng of genomic DNA per fly, and DNA quality was adequate for standard PCR applications. In principle, our tissue homogenizer can be used for isolation of DNA suitable for library production and high throughput genotyping by Multiplexed Shotgun Genotyping (MSG), as well as RNA isolation from single flies. The sample adapter can also hold and shake other items, such as centrifuge tubes (15–50 mL) or small bottles. PMID:26818699

  8. Streptomycetes in micro-cultures: growth, production of secondary metabolites, and storage and retrieval in the 96-well format.

    PubMed

    Minas, W; Bailey, J E; Duetz, W

    2000-12-01

    Mycelium-forming Streptomyces strains were grown in one milliliter liquid micro-cultures in square deep-well microtiter plates. Growth was evaluated with respect to biomass formation and production of secondary metabolites which were found to be very similar in the micro-cultures, bioreactor, and shake flask cultivations, respectively. Despite repetitive sampling and extensive growth on the walls of the wells, no cross contamination occurred. Furthermore, we successfully employed cold storage at -20 degrees C of spore suspensions (in the 96-well format), directly prepared from cultures grown on agar in the microtitre plate. Cultures were retrieved by replicating aliquots from the frozen spore suspensions.

  9. HIGHLY SENSITIVE ASSAY FOR ANTICHOLINESTERASE COMPOUNDS USING 96 WELL PLATE FORMAT

    EPA Science Inventory

    One of the approaches for reducing uncertainties in the assessment of human exposure is to better characterize concentrations of hazardous compounds that may be present in our immediate environment. A significant limitation to this approach, however, is that sampling and labora...

  10. Development of a 96-well plate iodine binding assay for amylose content determination

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cereal starch amylose/amylopectin (AM/AP) ratios are critical in functional properties for food and industrial applications. Conventional methods for the determination of AM/AP of cereal starches are very time consuming and labor intensive making it very difficult to screen large sample sets. Stud...

  11. Determining cereal starch amylose content using a dual wavelength iodine binding 96 well plate assay

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cereal starch amylose/amylopectin (AM/AP) ratios are critical in functional properties for food and industrial applications. Conventional determination of AM/AP of cereal starches are very time consuming and labor intensive making it very difficult to screen large sample sets. Studying these large...

  12. Folate content in fresh-cut vegetable packed products by 96-well microtiter plate microbiological assay.

    PubMed

    Fajardo, Violeta; Alonso-Aperte, Elena; Varela-Moreiras, Gregorio

    2015-02-15

    Ready-to-eat foods have nowadays become a significant portion of the diet. Accordingly, nutritional composition of these food categories should be well-known, in particular its folate content. However, there is a broad lack of folate data in food composition tables and databases. A total of 21 fresh-cut vegetable and fruit packed products were analysed for total folate (TF) content using a validated method that relies on the folate-dependent growth of chloramphenicol-resistant Lactobacillus casei subspecies rhamnosus (NCIMB 10463). Mean TF content ranged from 10.0 to 140.9μg/100g for the different matrices on a fresh weight basis. Higher TF quantity, 140.9-70.1μg/100g, was found in spinach, rocket, watercress, chard and broccoli. Significant differences were observed between available data for fresh vegetables and fruits from food composition tables or databases and the analysed results for fresh-cut packed products. Supplied data support the potential of folate-rich fresh-cut ready-to-eat vegetables to increase folate intake significantly.

  13. License Plate Recognition System for Indian Vehicles

    NASA Astrophysics Data System (ADS)

    Sanap, P. R.; Narote, S. P.

    2010-11-01

    We consider the task of recognition of Indian vehicle number plates (also called license plates or registration plates in other countries). A system for Indian number plate recognition must cope with wide variations in the appearance of the plates. Each state uses its own range of designs with font variations between the designs. Also, vehicle owners may place the plates inside glass covered frames or use plates made of nonstandard materials. These issues compound the complexity of automatic number plate recognition, making existing approaches inadequate. We have developed a system that incorporates a novel combination of image processing and artificial neural network technologies to successfully locate and read Indian vehicle number plates in digital images. Commercial application of the system is envisaged.

  14. Practical automatic Arabic license plate recognition system

    NASA Astrophysics Data System (ADS)

    Mohammad, Khader; Agaian, Sos; Saleh, Hani

    2011-02-01

    Since 1970's, the need of an automatic license plate recognition system, sometimes referred as Automatic License Plate Recognition system, has been increasing. A license plate recognition system is an automatic system that is able to recognize a license plate number, extracted from image sensors. In specific, Automatic License Plate Recognition systems are being used in conjunction with various transportation systems in application areas such as law enforcement (e.g. speed limit enforcement) and commercial usages such as parking enforcement and automatic toll payment private and public entrances, border control, theft and vandalism control. Vehicle license plate recognition has been intensively studied in many countries. Due to the different types of license plates being used, the requirement of an automatic license plate recognition system is different for each country. [License plate detection using cluster run length smoothing algorithm ].Generally, an automatic license plate localization and recognition system is made up of three modules; license plate localization, character segmentation and optical character recognition modules. This paper presents an Arabic license plate recognition system that is insensitive to character size, font, shape and orientation with extremely high accuracy rate. The proposed system is based on a combination of enhancement, license plate localization, morphological processing, and feature vector extraction using the Haar transform. The performance of the system is fast due to classification of alphabet and numerals based on the license plate organization. Experimental results for license plates of two different Arab countries show an average of 99 % successful license plate localization and recognition in a total of more than 20 different images captured from a complex outdoor environment. The results run times takes less time compared to conventional and many states of art methods.

  15. Development of a rapid, 96-well alkaline based differential DNA extraction method for sexual assault evidence.

    PubMed

    Hudlow, William R; Buoncristiani, Martin R

    2012-01-01

    We present a rapid alkaline lysis procedure for the extraction of DNA from sexual assault evidence that generates purified sperm fraction extracts that yield STR typing results similar to those obtained from the traditional organic/dithiothreitol differential extraction. Specifically, a sodium hydroxide based differential extraction method has been developed in a single-tube format and further optimized in a 96-well format. The method yields purified extracts from a small sample set (≈ 2-6 swabs) in approximately 2h and from a larger sample set (up to 96 swabs) in approximately 4h. While conventional differential extraction methods require vigorous sample manipulation to remove the spermatozoa from the substrate, the method described here exploits the propensity of sperm to adhere to a substrate and does not require any manipulation of the substrate after it is sampled. For swabs, sample handling is minimized by employing a process where the tip of the swab, including the shaft, is transferred to the appropriate vessel eliminating the need for potentially hazardous scalpels to separate the swab material from the shaft. The absence of multiple handling steps allows the process to be semi-automated, however the procedure as described here does not require use of a robotic system. This method may provide forensic laboratories a cost-effective tool for the eradication of backlogs of sexual assault evidence, and more timely service to their client agencies. In addition, we have demonstrated that a modification of the procedure can be used to retrieve residual sperm-cell DNA from previously extracted swabs.

  16. Lighting system with heat distribution face plate

    DOEpatents

    Arik, Mehmet; Weaver, Stanton Earl; Stecher, Thomas Elliot; Kuenzler, Glenn Howard; Wolfe, Jr., Charles Franklin; Li, Ri

    2013-09-10

    Lighting systems having a light source and a thermal management system are provided. The thermal management system includes synthetic jet devices, a heat sink and a heat distribution face plate. The synthetic jet devices are arranged in parallel to one and other and are configured to actively cool the lighting system. The heat distribution face plate is configured to radially transfer heat from the light source into the ambient air.

  17. Enzyme activity assay of glycoprotein enzymes based on a boronate affinity molecularly imprinted 96-well microplate.

    PubMed

    Bi, Xiaodong; Liu, Zhen

    2014-12-16

    Enzyme activity assay is an important method in clinical diagnostics. However, conventional enzyme activity assay suffers from apparent interference from the sample matrix. Herein, we present a new format of enzyme activity assay that can effectively eliminate the effects of the sample matrix. The key is a 96-well microplate modified with molecularly imprinted polymer (MIP) prepared according to a newly proposed method called boronate affinity-based oriented surface imprinting. Alkaline phosphatase (ALP), a glycoprotein enzyme that has been routinely used as an indicator for several diseases in clinical tests, was taken as a representative target enzyme. The prepared MIP exhibited strong affinity toward the template enzyme (with a dissociation constant of 10(-10) M) as well as superb tolerance for interference. Thus, the enzyme molecules in a complicated sample matrix could be specifically captured and cleaned up for enzyme activity assay, which eliminated the interference from the sample matrix. On the other hand, because the boronate affinity MIP could well retain the enzymatic activity of glycoprotein enzymes, the enzyme captured by the MIP was directly used for activity assay. Thus, additional assay time and possible enzyme or activity loss due to an enzyme release step required by other methods were avoided. Assay of ALP in human serum was successfully demonstrated, suggesting a promising prospect of the proposed method in real-world applications.

  18. A Microfabricated 96-Well 3D Assay Enabling High-Throughput Quantification of Cellular Invasion Capabilities

    PubMed Central

    Hao, Rui; Wei, Yuanchen; Li, Chaobo; Chen, Feng; Chen, Deyong; Zhao, Xiaoting; Luan, Shaoliang; Fan, Beiyuan; Guo, Wei; Wang, Junbo; Chen, Jian

    2017-01-01

    This paper presents a 96-well microfabricated assay to study three-dimensional (3D) invasion of tumor cells. A 3D cluster of tumor cells was first generated within each well by seeding cells onto a micro-patterned surface consisting of a central fibronectin-coated area that promotes cellular attachment, surrounded by a poly ethylene glycol (PEG) coated area that is resistant to cellular attachment. Following the formation of the 3D cell clusters, a 3D collagen extracellular matrix was formed in each well by thermal-triggered gelation. Invasion of the tumor cells into the extracellular matrix was subsequently initiated and monitored. Two modes of cellular infiltration were observed: A549 cells invaded into the extracellular matrix following the surfaces previously coated with PEG molecules in a pseudo-2D manner, while H1299 cells invaded into the extracellular matrix in a truly 3D manner including multiple directions. Based on the processing of 2D microscopic images, a key parameter, namely, equivalent invasion distance (the area of invaded cells divided by the circumference of the initial cell cluster) was obtained to quantify migration capabilities of these two cell types. These results validate the feasibility of the proposed platform, which may function as a high-throughput 3D cellular invasion assay. PMID:28240272

  19. A Microfabricated 96-Well 3D Assay Enabling High-Throughput Quantification of Cellular Invasion Capabilities.

    PubMed

    Hao, Rui; Wei, Yuanchen; Li, Chaobo; Chen, Feng; Chen, Deyong; Zhao, Xiaoting; Luan, Shaoliang; Fan, Beiyuan; Guo, Wei; Wang, Junbo; Chen, Jian

    2017-02-27

    This paper presents a 96-well microfabricated assay to study three-dimensional (3D) invasion of tumor cells. A 3D cluster of tumor cells was first generated within each well by seeding cells onto a micro-patterned surface consisting of a central fibronectin-coated area that promotes cellular attachment, surrounded by a poly ethylene glycol (PEG) coated area that is resistant to cellular attachment. Following the formation of the 3D cell clusters, a 3D collagen extracellular matrix was formed in each well by thermal-triggered gelation. Invasion of the tumor cells into the extracellular matrix was subsequently initiated and monitored. Two modes of cellular infiltration were observed: A549 cells invaded into the extracellular matrix following the surfaces previously coated with PEG molecules in a pseudo-2D manner, while H1299 cells invaded into the extracellular matrix in a truly 3D manner including multiple directions. Based on the processing of 2D microscopic images, a key parameter, namely, equivalent invasion distance (the area of invaded cells divided by the circumference of the initial cell cluster) was obtained to quantify migration capabilities of these two cell types. These results validate the feasibility of the proposed platform, which may function as a high-throughput 3D cellular invasion assay.

  20. Phospholipid removal combined with a semi-automated 96-well SPE application for determination of budesonide in human plasma with LC-MS/MS.

    PubMed

    Nilsson, Karin; Andersson, Maria; Beck, Olof

    2014-11-01

    A semi-automated method for quantification of budesonide in human plasma was developed, validated, and applied for high-volume analysis of samples in connection with a pharmacokinetic study. Protein and phospholipid removal was performed using an Ostro 96-well filter plate and subsequently combined with C18 solid-phase extraction on a Hamilton Microlab STARlet automation robot. The final extracts were evaporated to dryness and redissolved in 20% acetonitrile/water. The procedure used budesonide-d8 as internal standard and gave a 3.5-fold concentration of plasma to extract. The final extracts (5 μL injected) were analyzed with selected reaction monitoring liquid chromatography-tandem mass spectrometry (LC-MS/MS) using electrospray ionization in positive mode. The chromatography system used a 100 mm ACQUITY BEH UPLC column and a gradient system consisting of aqueous 0.1% formic acid and acetonitrile as organic modifier. Phospholipid removal was found to be needed during method development in order to reduce ion suppression effects from matrix and to increase method sensitivity. The measuring range was 50-5000 pg/mL with and LOD 24 pg/mL. Calibration response showed good linearity (correlation coefficients<0.99) over the measuring range. The absolute recovery over the sample preparation procedure was estimated to 67%. Total imprecision was <9% at three levels and accuracy was between 98.9 and 103%. The method was successfully applied for analysis of 864 study samples in a short time. The quality control samples at concentration levels 200 and 2000 pg/mL gave a total imprecision of 7.4% and 4.2%, respectively, (n=95).

  1. Development and Testing of a Colorimetric 96 Well Plate Assay for the Determination of HD Hydrolysis Rate in Various Formulations

    DTIC Science & Technology

    2005-01-01

    Cold Foam 1% ethylene glycol 1% benzalkonium chloride 1% polyvinylpolypyrrolidone 1% Brij 58 1% Benzyldimethyl tetradecyl ammonium chloride dihydrate...LITERA TURE CITED ..................................................................................... 15 5 FIGURES 1. Rates of Chloride Release in...Ground, MD). The HD was stabilized with tributylamine and was approximately 90% pure. Quantitative assays were conducted with a chloride electrode

  2. A 96-well single-pot protein precipitation, liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the determination of muraglitazar, a novel diabetes drug, in human plasma.

    PubMed

    Xue, Y-J; Liu, Jane; Pursley, Janice; Unger, Steve

    2006-02-02

    A 96-well single-pot protein precipitation, liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed and validated for the determination of muraglitazar, a PPAR alpha/gamma dual agonist, in human plasma. The internal standard, a chemical analogue, was dissolved in acetonitrile containing 0.1% formic acid. The solvent system was also served as a protein precipitation reagent. Human plasma samples (0.1 mL) and the internal standard solution (0.3 mL) were added to a 96-well plate. The plate was vortexed for 1 min and centrifuged for 5 min. Then the supernatant layers were directly injected into the LC/MS/MS system. The chromatographic separation was achieved isocratically on a Phenomenox C18(2) Luna column (2 mm x 50 mm, 5 microm). The mobile phase contained 20/80 (v/v) of water and acetonitrile containing 0.1% formic acid. Detection was by positive ion electrospray tandem mass spectrometry on a Sciex API 3000. The standard curve, which ranged from 1 to 1000 ng/mL, was fitted to a 1/x weighted quadratic regression model. This single-pot approach effectively eliminated three time consuming sample preparation steps: sample transfer, dry-down, and reconstitution before the injection, while it preserved all the benefits of the traditional protein precipitation. By properly adjusting the autosampler needle offset level, only the supernatant was injected, without disturbing the precipitated proteins in the bottom. As a result, the quality of chromatography and column life were not compromised. After more than 600 injections, there was only slightly increase of column back-pressure. The validation results demonstrated that this method was rugged and provide satisfactory precision and accuracy. The method has been successfully applied to analyze human plasma samples in support of a first-in-man study. This method has also been validated in monkey and mouse plasma for the determination of muraglitazar.

  3. Higher throughput bioanalysis by automation of a protein precipitation assay using a 96-well format with detection by LC-MS/MS.

    PubMed

    Watt, A P; Morrison, D; Locker, K L; Evans, D C

    2000-03-01

    Generic methodology for the automated preparation and analysis of drug levels in plasma samples within a drug discovery environment was achieved through the redesign of a protein precipitation assay to a microtiter (96-well) plate format and the application of robotic liquid handling for performance of all transfer and pipetting steps. Validation studies revealed that the application of robotics to sample preparation, in general, maintained the analytical accuracy and precision compared with preparing samples manually. The use of rapid gradient LC-MS/MS for analysis coupled with flow diversion of the solvent front allowed the introduction of protein-precipitated samples into the mass spectrometer without the necessity for source cleaning. The problem inherent in automatically pipetting plasma, caused by fibrinogen clots, was overcome by storing samples at -80 degrees C and thus precluding clot formation. The resulting methodology allowed sample preparation for a 96-well plate designed to accommodate 54 unknowns, duplicate 12-point calibration curves, and 6 sets of quality controls at three levels in approximately 2 h. This approach allowed an increase in throughput of sample preparation and analysis to >400 samples per day per LC-MS/MS instrument with minimal manual intervention. Overall, substantial time savings were realized, demonstrating that automation is an increasingly essential tool in a drug discovery bioanalytical environment.

  4. Parallel Plate System for Collecting Data Used to Determine Viscosity

    NASA Technical Reports Server (NTRS)

    Kaukler, William (Inventor); Ethridge, Edwin C. (Inventor)

    2013-01-01

    A parallel-plate system collects data used to determine viscosity. A first plate is coupled to a translator so that the first plate can be moved along a first direction. A second plate has a pendulum device coupled thereto such that the second plate is suspended above and parallel to the first plate. The pendulum device constrains movement of the second plate to a second direction that is aligned with the first direction and is substantially parallel thereto. A force measuring device is coupled to the second plate for measuring force along the second direction caused by movement of the second plate.

  5. A 96-well single-pot liquid-liquid extraction, hydrophilic interaction liquid chromatography-mass spectrometry method for the determination of muraglitazar in human plasma.

    PubMed

    Xue, Y-J; Liu, Jane; Unger, Steve

    2006-06-07

    A single-pot liquid-liquid extraction (LLE) with hydrophilic interaction liquid chromatography/tandem mass spectrometry (HILIC/MS/MS) method has been developed and validated for the determination of muraglitazar, a hydrophobic diabetes drug, in human plasma. To 0.050 ml of each plasma sample in a 96-well plate, the internal standard solution in acetonitrile and toluene were added to extract the compound of interest. The plate was vortexed, followed by centrifugation. The organic layer was then directly injected into an LC/MS/MS system. Chromatographic separation was achieved isocratically on a Thermohypersil_Keystone, Hypersil silica column (3 mmx50 mm, 3 microm). The mobile phase contained 85% of methyl t-butyl ether and 15% of 90/10 (v/v) acetonitrile/water with 0.3% trifluoroacetic acid. Post-column mobile phase of 50/50 (v/v) acetonitrile/water containing 0.1% formic acid was added. Detection was by positive ion electrospray tandem mass spectrometry on a Sciex API 4000. The standard curve, ranged from 1 to 1000 ng/ml, was fitted to a 1/x weighted quadratic regression model. This single-pot LLE approach effectively eliminated time-consuming organic layer transfer, dry-down, and sample reconstitution steps, which are essential for a conventional liquid-liquid extraction procedure. The modified mobile phase was more compatible with the direct injection of the commonly used extraction solvents in LLE. Furthermore, the modified mobile phase improved the retention of muraglitazar, a hydrophobic compound, on the normal phase silica column. The validation results demonstrated that this method was rugged and suitable for analyzing muraglitazar in human plasma. In comparison with a revised-phase LC/MS/MS method, this single-pot LLE, HILIC/MS/MS method improved the detection sensitivity by more than four-fold based upon the LLOQ signal to noise ratio. This approach may be applied to other hydrophobic compounds with proper modification of the mobile phase compositions.

  6. Root-Contact/Pressure-Plate Assembly For Hydroponic System

    NASA Technical Reports Server (NTRS)

    Morris, Carlton E.; Loretan, Philip A.; Bonsi, Conrad K.; Hill, Walter A.

    1994-01-01

    Hydroponic system includes growth channels equipped with rootcontact/pressure-plate assemblies. Pump and associated plumbing circulate nutrient liquid from reservoir, along bottom of growth channels, and back to reservoir. Root-contact/pressure-plate assembly in each growth channel stimulates growth of roots by applying mild contact pressure. Flat plate and plate connectors, together constitute pressure plate, free to move upward to accommodate growth of roots. System used for growing sweetpotatoes and possibly other tuber and root crops.

  7. A semi-automated 96-well protein precipitation method for the determination of montelukast in human plasma using high performance liquid chromatography/fluorescence detection.

    PubMed

    Kitchen, Chester J; Wang, Amy Q; Musson, Donald G; Yang, Amy Y; Fisher, Alison L

    2003-03-26

    A simple, semi-automated, protein precipitation assay for the determination of montelukast (SINGULAIR, MK-0476) in human plasma has been developed. Montelukast is a potent and selective antagonist of the cysteinyl leukotriene receptor used for the treatment of asthma. A Packard MultiPROBE II EX is used to transfer 300 microl of plasma from sample, standard, and QC sample tubes to a microtiter plate (96-well). After addition of the internal standard by a repeating pipettor, a Tomtec QUADRA 96 adds 400 microl of acetonitrile to all plasma sample wells, simultaneously, in the microtiter plate. The Tomtec is also used to transfer the acetonitrile supernatant from the plasma protein precipitation step, batchwise, to another microtiter plate for analysis by HPLC with fluorescence detection. This assay has been validated and implemented for a clinical study of over 1300 plasma samples and is comparable to manual assays in the LLOQ (lower limit of quantitation, 3 ng/ml) and in stability. This is the first semi-automated protein precipitation assay published for the analysis of montelukast in human plasma and it results in significant time savings over the manual methods, both in sample preparation and in HPLC run time.

  8. Automated 96-well solid phase extraction and hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the analysis of cetirizine (ZYRTEC) in human plasma--with emphasis on method ruggedness.

    PubMed

    Song, Qi; Junga, Heiko; Tang, Yong; Li, Austin C; Addison, Tom; McCort-Tipton, Melanie; Beato, Brian; Naidong, Weng

    2005-01-05

    A high-throughput bioanalytical method based on automated sample transfer, automated solid phase extraction, and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) analysis, has been developed for the determination of cetirizine, a selective H(1)-receptor antagonist. Deuterated cetirizine (cetirizine-d(8)) was synthesized as described and was used as the internal standard. Samples were transferred into 96-well plates using an automated sample handling system. Automated solid phase extraction was carried out using a 96-channel programmable liquid-handling workstation. Solid phase extraction 96-well plate on polymer sorbent (Strata X) was used to extract the analyte. The extracted samples were injected onto a Betasil silica column (50 x 3, 5 microm) using a mobile phase of acetonitrile-water-acetic acid-trifluroacetic acid (93:7:1:0.025, v/v/v/v) at a flow rate of 0.5 ml/min. The chromatographic run time is 2.0 min per injection, with retention time of cetirizine and cetirizine-d(8) both at 1.1 min. The system consisted of a Shimadzu HPLC system and a PE Sciex API 3000 or API 4000 tandem mass spectrometer with (+) ESI. The method has been validated over the concentration range of 1.00-1000 ng/ml cetirizine in human plasma, based on a 0.10-ml sample size. The inter-day precision and accuracy of the quality control (QC) samples demonstrated <3.0% relative standard deviation (R.S.D.) and <6.0% relative error (RE). Stability of cetirizine in stock solution, in plasma, and in reconstitution solution was established. The absolute extraction recovery was 85.8%, 84.5%, and 88.0% at 3, 40, and 800 ng/ml, respectively. The recovery for the internal standard was 84.1%. No adverse matrix effects were noticed for this assay. The automation of the sample preparation steps not only increased the analysis throughput, but also increased method ruggedness. The use of a stable isotope-labeled internal standard further improved the method ruggedness

  9. Voltage-current characteristics of a pin-plate system with different plate configurations

    NASA Astrophysics Data System (ADS)

    Feng, Zhuangbo; Long, Zhengwei

    2013-03-01

    In this paper, the voltage-current (V-I) characteristics of a pin-plate system with four types of collection plate configurations are studied experimentally. The collection plates consider a single metal plate, a metal plate with a fly ash cake layer, a metal plate with a clean filter media and a metal plate with a dirty filter media. The results show that the clean filter media has no obvious effect on the V-I characteristics. But the dirty filter media reduces the current density because of its high resistance. The thick fly ash cake layer increase current density because of the anti-corona effect but the increment is not very obvious.

  10. Numerically simulating the sandwich plate system structures

    NASA Astrophysics Data System (ADS)

    Feng, Guo-Qing; Li, Gang; Liu, Zhi-Hui; Niu, Huai-Lei; Li, Chen-Feng

    2010-09-01

    Sandwich plate systems (SPS) are advanced materials that have begun to receive extensive attention in naval architecture and ocean engineering. At present, according to the rules of classification societies, a mixture of shell and solid elements are required to simulate an SPS. Based on the principle of stiffness decomposition, a new numerical simulation method for shell elements was proposed. In accordance with the principle of stiffness decomposition, the total stiffness can be decomposed into the bending stiffness and shear stiffness. Displacement and stress response related to bending stiffness was calculated with the laminated shell element. Displacement and stress response due to shear was calculated by use of a computational code write by FORTRAN language. Then the total displacement and stress response for the SPS was obtained by adding together these two parts of total displacement and stress. Finally, a rectangular SPS plate and a double-bottom structure were used for a simulation. The results show that the deflection simulated by the elements proposed in the paper is larger than the same simulated by solid elements and the analytical solution according to Hoff theory and approximate to the same simulated by the mixture of shell-solid elements, and the stress simulated by the elements proposed in the paper is approximate to the other simulating methods. So compared with calculations based on a mixture of shell and solid elements, the numerical simulation method given in the paper is more efficient and easier to do.

  11. Composite Bipolar Plate for Unitized Fuel Cell/Electrolyzer Systems

    NASA Technical Reports Server (NTRS)

    Mittelsteadt, Cortney K.; Braff, William

    2009-01-01

    In a substantial improvement over present alkaline systems, an advanced hybrid bipolar plate for a unitized fuel cell/electrolyzer has been developed. This design, which operates on pure feed streams (H2/O2 and water, respectively) consists of a porous metallic foil filled with a polymer that has very high water transport properties. Combined with a second metallic plate, the pore-filled metallic plates form a bipolar plate with an empty cavity in the center.

  12. Tectonics of oblique plate boundary systems

    NASA Astrophysics Data System (ADS)

    Díaz-Azpiroz, Manuel; Brune, Sascha; Leever, Karen A.; Fernández, Carlos; Czeck, Dyanna M.

    2016-12-01

    The relative displacement between lithospheric plates normally results in obliquely deforming plate boundaries. This is simply caused by the fact that, on plate tectonics basis, irregularly shaped plate boundaries are rarely perpendicular or parallel to small-circle rotation paths, which describe plate motion on a sphere (Fig. 1a). Global current relative plate motions estimated from geological data (DeMets et al., 2010; Argus et al., 2011) and GPS measurements (e.g., Kreemer et al., 2003; Argus et al., 2010) provide insight to the prevalent degrees of obliquity on Earth's surface. Based on these global data sets, Philippon and Corti (2016), statistically show that current orthogonal boundaries (obliquity angle smaller than 10°) represent around 8% of the total boundary length whereas strike-slip boundaries (obliquity angle larger than 80°) are encountered in < 10% of the total boundary length. Therefore, around 80% of active plate boundaries present oblique relative motions. Furthermore, changes in plate kinematics leading to migration or jumps in the rotation poles necessarily cause obliquity along former pure strike-slip or convergent/divergent boundaries (Fig. 1b).

  13. Development and validation of a 96-well cellular assay for the discovery of ALDH1A1 inhibitors.

    PubMed

    Ming, Wenyu; Ma, Wenzhen; Chen, Lisa H; Volk, Catherine; Michael, Mervyn Dodson; Xu, Yanping; Zhang, Fang; Wang, Xiaojun

    2013-07-01

    Retinoic acid, the active metabolite of vitamin A, plays important roles in various physiological and pathological processes. The two-step production of retinoic acid from vitamin A (retinol) is catalyzed by alcohol dehydrogenases and aldehyde dehydrogenases, which are potential therapeutic targets for numerous diseases, such as obesity, diabetes, and cancer. Currently, the lack of a suitable high-throughput cellular assay hinders efforts to identify therapeutic small molecular inhibitors of aldehyde dehydrogenase, such as ALDH1A1. In this report, we utilized high-content imaging technology and a commercially available cell permeable ALDH substrate to develop a 96-well cellular ALDH1A1 assay. This assay has a robust and sensitive readout and is amenable to automation. With this cellular assay, we identified potent selective ALDH1A1 inhibitors to explore the role of retinoic acid production in various preclinical disease models.

  14. Orion Boiler Plate Airdrop Test System

    NASA Technical Reports Server (NTRS)

    Machin, Ricardo A.; Evans, Carol T.

    2013-01-01

    On the 29th of February 2012 the Orion Capsule Parachute Assembly System (CPAS) project attempted to perform an airdrop test of a boilerplate test article for the second time. The first attempt (Cluster Development Test 2, July 2008) to deliver a similar boilerplate from a C-17 using the Low Velocity Air Drop (LVAD) technique resulted in the programmer parachute failing to properly inflate, the test article failing to achieve the desired test initiation conditions, and the test article a total loss. This paper will pick up where the CDT-2 failure investigation left off, describing the test technique that was adopted, and outline the modeling that was performed to gain confidence that the second attempt would be successful. The second boiler plate test (Cluster Development Test 3-3) was indeed a complete success and has subsequently been repeated several times, allowing the CPAS project to proceed with the full scale system level development testing required to integrate the hardware to the first Entry Flight Test vehicle as well as go into the Critical Design Review with minimum risk and a mature design.

  15. Simplified method for determination of clarithromycin in human plasma using protein precipitation in a 96-well format and liquid chromatography-tandem mass spectrometry.

    PubMed

    Shin, Jaekyu; Pauly, Daniel F; Johnson, Julie A; Frye, Reginald F

    2008-08-01

    A simplified method to determine clarithromycin concentrations in human plasma using protein precipitation in a 96-well plate and liquid chromatography-tandem mass spectrometry was developed and validated. Plasma proteins were precipitated with acetonitrile and roxithromycin was used as the internal standard. After vortex mixing and centrifugation, the supernatants were directly injected onto a Phenomenex Luna Phenyl-Hexyl column (50 mm x 2.0 mm ID, 3 microm). The mobile phase consisted of water and methanol (30:70, v/v) containing 0.1% formic acid and 5mM ammonium acetate. The flow rate was 0.22 mL/min and the total run time (injection to injection) was less than 3 min. Detection of the analytes was achieved using positive ion electrospray tandem mass spectrometry in selected reaction monitoring (SRM) mode. The linear standard curve ranged from 100 to 5000 ng/mL and the precision and accuracy (inter- and intra-run) were within 7.9% and 4.9%, respectively. The method was successfully used to determine clarithromycin concentrations in human plasma samples obtained from healthy subjects who were given clarithromycin 500 mg for 3 days. The method is rapid, simple, precise and directly applicable to clarithromycin pharmacokinetic studies.

  16. Remediation System Evaluation, Peerless Plating Site

    EPA Pesticide Factsheets

    The Peerless Plating Superfund Site is located at 2554 South Getty Street, north of the intersection of South Getty Street and East Sherman Boulevard in Muskegon, Michigan. Copper, nickel, chromium, cadmium, and zinc electroplating operations as well as...

  17. Plate Tectonics, Geographical Information System, paleogeography

    SciTech Connect

    Moore, Thomas L.; Scotese, Christopher

    2002-05-24

    The PaleoX.framwork is a dynamically linked/loaded framework for Cocoa applications. The primary goal of this library is to standardize several elements used for working with paleogeographic data. This includes objects designed to organize information for tectonic plates, including maps, rotation objects, plate names, and designations. In addition, PaleoX provides object-oriented solutions for handling standard paleogeographic file formats from the PALEOMAP Project.

  18. Ornament Problem Suppression in Indonesian License Plate Recognition Systems

    NASA Astrophysics Data System (ADS)

    Mahatmaputra Tedjojuwono, Samuel

    2017-03-01

    Based on the original work of fast performance algorithm in detecting Indonesian license plate, the proposed work will solve the error found in the license plate localization process caused by plate like pattern within the image, which was called the ornament problem. Although not in all cases, this problem could exist when a car has banner, regular pattern, car’s front grill, that could miss understood by the system as license plate letters. The proposed work will implement filtering systems instead of machine learning approach. The filtering methods will follows three steps: detection filter based on the number of elements in the vector, based on the letter proportion of a license plate number, and based on the distance between detected letters. This approach will maintain the fast properties of the original algorithm and will increase the accuracy of localizing the license plate within the given image.

  19. Note: Heated flyer-plate impact system.

    PubMed

    Dolan, D H; Seagle, C T; Ao, T; Hacking, R G

    2014-07-01

    A technique for launching heated flyer plates was developed on a single-stage gas gun. This type of impact creates a well-posed mechanical state and a tunable thermal state, which is useful for calibrating dynamic temperature measurements. Proof-of-principle thermoreflectance measurements were performed using this technique. Since the target remains at room temperature until the moment of impact, heated flyers avoid differential expansion and annealing issues, allowing novel impact experiments to be performed.

  20. Absolute viscosity measured using instrumented parallel plate system

    NASA Technical Reports Server (NTRS)

    Broyles, H. H.

    1967-01-01

    An automatic system measures the true average shear viscosity of liquids and viscoelastic materials, using the parallel plate method and automatically displays the results on a graphic record. This eliminates apparatus setup and extensive calculations.

  1. Global Plate Velocities from the Global Positioning System

    NASA Technical Reports Server (NTRS)

    Larson, Kristine M.; Freymueller, Jeffrey T.; Philipsen, Steven

    1997-01-01

    We have analyzed 204 days of Global Positioning System (GPS) data from the global GPS network spanning January 1991 through March 1996. On the basis of these GPS coordinate solutions, we have estimated velocities for 38 sites, mostly located on the interiors of the Africa, Antarctica, Australia, Eurasia, Nazca, North America, Pacific, and South America plates. The uncertainties of the horizontal velocity components range from 1.2 to 5.0 mm/yr. With the exception of sites on the Pacific and Nazca plates, the GPS velocities agree with absolute plate model predictions within 95% confidence. For most of the sites in North America, Antarctica, and Eurasia, the agreement is better than 2 mm/yr. We find no persuasive evidence for significant vertical motions (less than 3 standard deviations), except at four sites. Three of these four were sites constrained to geodetic reference frame velocities. The GPS velocities were then used to estimate angular velocities for eight tectonic plates. Absolute angular velocities derived from the GPS data agree with the no net rotation (NNR) NUVEL-1A model within 95% confidence except for the Pacific plate. Our pole of rotation for the Pacific plate lies 11.5 deg west of the NNR NUVEL-1A pole, with an angular speed 10% faster. Our relative angular velocities agree with NUVEL-1A except for some involving the Pacific plate. While our Pacific-North America angular velocity differs significantly from NUVEL-1A, our model and NUVEL-1A predict very small differences in relative motion along the Pacific-North America plate boundary itself. Our Pacific-Australia and Pacific- Eurasia angular velocities are significantly faster than NUVEL-1A, predicting more rapid convergence at these two plate boundaries. Along the East Pacific Pise, our Pacific-Nazca angular velocity agrees in both rate and azimuth with NUVFL-1A.

  2. System recognizing Bahamian license plate with touching characters

    NASA Astrophysics Data System (ADS)

    Dun, Jingyu; Zhang, Sanyuan; Ye, Xiuzi; Zhang, Yin

    2016-11-01

    Various methods are proposed for license plate recognition, but none of them are universal. Some common methods for license plate localization, character extraction, and recognition are analyzed. Then a system is proposed to recognize the Bahamian license plate with touching characters. A vertical edge-based method with a modified sliding window technique is used to locate the license plate, and a machine learning process is used to trim the region. The located license plate is rectified by using the minimum enclosing box and the stroke width value. Then the vertical projection and pairs of extreme points are combined to segment the characters. Finally, a deep learning method is used to recognize the characters. 2996 images are experimented on and the total recognition accuracy achieves 83.29%. Typical methods of each stage are implemented to compare with the proposed methods. In addition, the proposed system is experimented on a public dataset to show the generalization ability of the system. The experimental results show that the proposed system performs better than the other methods and is able to be used in a real-time application.

  3. Imaging and quantitative analysis of tritium-labelled cells in lymphocyte proliferation assays using microchannel plate detectors originally developed for X-ray astronomy.

    PubMed

    Lees, J E; Hales, J M

    2001-01-01

    Microchannel plate detectors have been used in many astronomical X-ray telescopes. Recently we have begun to use similar detectors to image electron emission from radiolabelled biological assays. Here we show how a microchannel plate (MCP) detector can be used to image tritium uptake in T lymphocyte proliferation assays. Quantitative analysis using the MCP detector has the same sensitivity and speed as conventional liquid scintillation counter (LSC) analysis whilst obviating the need for scintillation fluid. In addition the system permits the imaging of whole plate harvests from a range of plate sizes. Here we present data obtained with 96-well plates and Terasaki plates.

  4. A simple 96-well liquid-liquid extraction with a mixture of acetonitrile and methyl t-butyl ether for the determination of a drug in human plasma by high-performance liquid chromatography with tandem mass spectrometry.

    PubMed

    Xue, Y-J; Pursley, Janice; Arnold, Mark E

    2004-02-04

    A simple 96-well plate liquid-liquid extraction (LLE), liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed and validated for the determination of a basic drug candidate in human plasma. Against the wisdom of conventional approaches, an aqueous/organic miscible solvent, acetonitrile, was used for liquid-liquid extraction along with methyl t-butyl ether. The use of acetonitrile effectively eliminated the formation of the irregular emulsion between aqueous/organic interfaces and modulated the polarity of the extraction solvents to achieve the desired recovery. This approach, which solved the emulsion problem, permitted the method to be automated using standard 96-well plate technology. A practical application was demonstrated through the use of this technique in the measurement of a novel drug in human plasma samples by LC/MS/MS. Chromatographic separation was achieved isocratically on a Phenomenox C18(2) Luna column (2 mm x 50 mm, 5 microm). The mobile phase contained 60% of 0.1% formic acid and 40% acetonitrile. Detection was by positive ion electrospray tandem mass spectrometry. The standard curve, which ranged from 1.22 to 979ng/ml, was fitted to a 1/x2 weighted quadratic regression model. The validation results show that this method was very rugged and had excellent precision and accuracy. The actual sample analysis results further demonstrated that this extraction procedure is well suited for real life applications. It is expected that with some modifications, this approach can be applied for the extraction of similar compounds from various biological fluids.

  5. Multiwell cell culture plate format with integrated microfluidic perfusion system

    NASA Astrophysics Data System (ADS)

    Domansky, Karel; Inman, Walker; Serdy, Jim; Griffith, Linda G.

    2006-01-01

    A new cell culture analog has been developed. It is based on the standard multiwell cell culture plate format but it provides perfused three-dimensional cell culture capability. The new capability is achieved by integrating microfluidic valves and pumps into the plate. The system provides a means to conduct high throughput assays for target validation and predictive toxicology in the drug discovery and development process. It can be also used for evaluation of long-term exposure to drugs or environmental agents or as a model to study viral hepatitis, cancer metastasis, and other diseases and pathological conditions.

  6. Plate-Based Fuel Processing System Final Report

    SciTech Connect

    Carlos Faz; Helen Liu; Jacques Nicole; David Yee

    2005-12-22

    On-board reforming of liquid fuels into hydrogen is an enabling technology that could accelerate consumer usage of fuel cell powered vehicles. The technology would leverage the convenience of the existing gasoline fueling infrastructure while taking advantage of the fuel cell efficiency and low emissions. Commercial acceptance of on-board reforming faces several obstacles that include: (1) startup time, (2) transient response, and (3) system complexity (size, weight and cost). These obstacles are being addressed in a variety of projects through development, integration and optimization of existing fuel processing system designs. In this project, CESI investigated steam reforming (SR), water-gas-shift (WGS) and preferential oxidation (PrOx) catalysts while developing plate reactor designs and hardware where the catalytic function is integrated into a primary surface heat exchanger. The plate reactor approach has several advantages. The separation of the reforming and combustion streams permits the reforming reaction to be conducted at a higher pressure than the combustion reaction, thereby avoiding costly gas compression for combustion. The separation of the two streams also prevents the dilution of the reformate stream by the combustion air. The advantages of the plate reactor are not limited to steam reforming applications. In a WGS or PrOx reaction, the non-catalytic side of the plate would act as a heat exchanger to remove the heat generated by the exothermic WGS or PrOx reactions. This would maintain the catalyst under nearly isothermal conditions whereby the catalyst would operate at its optimal temperature. Furthermore, the plate design approach results in a low pressure drop, rapid transient capable and attrition-resistant reactor. These qualities are valued in any application, be it on-board or stationary fuel processing, since they reduce parasitic losses, increase over-all system efficiency and help perpetuate catalyst durability. In this program, CESI

  7. Infinite systems in problems for a stiffened rectangular plate

    NASA Astrophysics Data System (ADS)

    Baburchenkov, M. F.; Borodachev, N. M.

    2016-07-01

    A method is proposed for obtaining analytic solutions of a set of infinite systems of linear algebraic equations arising in problems of elasticity for stiffened rectangular plates with stiffening ribs. The method is based on a transformation of a set of infinite systems to a single system and on determining a majorant of the function generating the system series with regard to the order of the unknowns. It is proved that the constructed solution satisfies the infinite system for large indices of the unknowns. The amount of computations is decreased, and the reliability of the results increases. Some realization examples are given.

  8. A dynamic simulation of a flat-plate collector system

    NASA Astrophysics Data System (ADS)

    Annino, A.

    1983-04-01

    A numerical model for the performance of a flat plate solar collector array is presented, with account taken of thermal transients and calculation on a microcomputer. The system modeled consists of a flat plate array, the heat transfer fluid, an insulated storage tank, an exchange loop for heating a secondary fluid, and a load maintained by a pump. The one-dimensional analysis includes equations for the energy balances, with consideration given to heat losses to the outside. A function is defined for the total incident solar radiation, and behavior is simulated over the entire 24-hr day, weighted by the highest and lowest recorded temperatures. Good agreement has been found with experimental data.

  9. A cost-effective plate-based sample preparation for antibody N-glycan analysis.

    PubMed

    Burnina, Irina; Hoyt, Erik; Lynaugh, Heather; Li, Huijuan; Gong, Bing

    2013-09-13

    During early cell line and process development of therapeutic antibodies, a cost-effective high-throughput approach to characterize the N-linked glycans is highly desired given that a large number of samples need to be analyzed. Using commercially available, low cost 96-well plates, we developed a practical procedure to prepare fluorescently labeled N-linked glycans for both qualitative and quantitative analysis by mass spectrometry (MS) and ultrahigh performance liquid chromatography (UPLC). Antibody samples were continuously denatured, reduced, and deglycosylated in a single 96-well hydrophobic membrane filter plate. Subsequently, released glycans were fluorescently labeled in a collection plate, and cleaned-up using a hydrophilic membrane filter plate. Carried out entirely in ready-to-use 96-well plates with simple buffer systems, this procedure requires less than 90min to finish. We applied the optimized procedure to examine the N-linked glycosylation of trastuzumab and were able to quantify ten major N-linked glycans. The results from different amounts of starting materials (10-200μg) were highly similar and showed the robustness of this procedure. Compared to other methods, this new procedure is simple to implement, economically more affordable, and could be very valuable for early screenings of antibody development.

  10. System Advisor Model: Flat Plate Photovoltaic Performance Modeling Validation Report

    SciTech Connect

    Freeman, J.; Whitmore, J.; Kaffine, L.; Blair, N.; Dobos, A. P.

    2013-12-01

    The System Advisor Model (SAM) is a free software tool that performs detailed analysis of both system performance and system financing for a variety of renewable energy technologies. This report provides detailed validation of the SAM flat plate photovoltaic performance model by comparing SAM-modeled PV system generation data to actual measured production data for nine PV systems ranging from 75 kW to greater than 25 MW in size. The results show strong agreement between SAM predictions and field data, with annualized prediction error below 3% for all fixed tilt cases and below 8% for all one axis tracked cases. The analysis concludes that snow cover and system outages are the primary sources of disagreement, and other deviations resulting from seasonal biases in the irradiation models and one axis tracking issues are discussed in detail.

  11. Sensor network based vehicle classification and license plate identification system

    SciTech Connect

    Frigo, Janette Rose; Brennan, Sean M; Rosten, Edward J; Raby, Eric Y; Kulathumani, Vinod K

    2009-01-01

    Typically, for energy efficiency and scalability purposes, sensor networks have been used in the context of environmental and traffic monitoring applications in which operations at the sensor level are not computationally intensive. But increasingly, sensor network applications require data and compute intensive sensors such video cameras and microphones. In this paper, we describe the design and implementation of two such systems: a vehicle classifier based on acoustic signals and a license plate identification system using a camera. The systems are implemented in an energy-efficient manner to the extent possible using commercially available hardware, the Mica motes and the Stargate platform. Our experience in designing these systems leads us to consider an alternate more flexible, modular, low-power mote architecture that uses a combination of FPGAs, specialized embedded processing units and sensor data acquisition systems.

  12. Convection pattern and stress system under the African plate

    NASA Technical Reports Server (NTRS)

    Liu, H.-S.

    1977-01-01

    Studies on tectonic forces from satellite-derived gravity data have revealed a subcrustal stress system which provides a unifying mechanism for uplift, depression, rifting, plate motion and ore formation in Africa. The subcrustal stresses are due to mantle convection. Seismicity, volcanicity and kimberlite magmatism in Africa and the development of the African tectonic and magnetic features are explained in terms of this single stress system. The tensional stress fields in the crust exerted by the upwelling mantle flows are shown to be regions of structural kinship characterized by major concentration of mineral deposits. It is probable that the space techniques are capable of detecting and determining the tectonic forces in the crust of Africa.

  13. The parameterization of microchannel-plate-based detection systems

    NASA Astrophysics Data System (ADS)

    Gershman, Daniel J.; Gliese, Ulrik; Dorelli, John C.; Avanov, Levon A.; Barrie, Alexander C.; Chornay, Dennis J.; MacDonald, Elizabeth A.; Holland, Matthew P.; Giles, Barbara L.; Pollock, Craig J.

    2016-10-01

    The most common instrument for low-energy plasmas consists of a top-hat electrostatic analyzer (ESA) geometry coupled with a microchannel-plate-based (MCP-based) detection system. While the electrostatic optics for such sensors are readily simulated and parameterized during the laboratory calibration process, the detection system is often less well characterized. Here we develop a comprehensive mathematical description of particle detection systems. As a function of instrument azimuthal angle, we parameterize (1) particle scattering within the ESA and at the surface of the MCP, (2) the probability distribution of MCP gain for an incident particle, (3) electron charge cloud spreading between the MCP and anode board, and (4) capacitive coupling between adjacent discrete anodes. Using the Dual Electron Spectrometers on the Fast Plasma Investigation on NASA's Magnetospheric Multiscale mission as an example, we demonstrate a method for extracting these fundamental detection system parameters from laboratory calibration. We further show that parameters that will evolve in flight, namely, MCP gain, can be determined through application of this model to specifically tailored in-flight calibration activities. This methodology provides a robust characterization of sensor suite performance throughout mission lifetime. The model developed in this work is not only applicable to existing sensors but also can be used as an analytical design tool for future particle instrumentation.

  14. Micro system comprising 96 micro valves on a titer plate

    NASA Astrophysics Data System (ADS)

    Krabbe, S.; Flitsch, D.; Büchs, J.; Schomburg, W. K.

    2016-10-01

    A system of 96 micro valves has been developed and mounted on top of a 48-well micro titer plate providing two valves for each well controlling its air inlet and outlet. Testing of the valve system showed that all valves are working and are opened and closed reliably. A pneumatic system is switching inlet and outlet valves independently of each other. The geometry of the feed channels ensures an equal air flow through all wells, when the valves are open. Between the micro valves, one optical fibre was inserted through the lid of each well allowing measuring the oxygen partial pressure in the enclosed air volume by fluorescence sensor spots. Escherichia coli bacteria were grown inside the wells and their metabolism was observed by the oxygen partial pressure change due to respiration. In all 48 wells, the same oxygen transfer rate was observed within an averaged standard deviation of 1 mmol/L/h. The oxygen transfer rate differences compared to a macroscopic standard shake flask system were overall compatible within their uncertainties.

  15. A combined lift and propulsion system of a steel plate by transverse flux linear induction motors

    SciTech Connect

    Hayashiya, H.; Ohsaki, H.; Masada, E.

    1999-09-01

    To realize a non-contacting conveyance of a steel plate, a combined lift and propulsion system of a steel plate by transverse flux linear induction motors (LIMs) is proposed. By introducing the DC biased AC feeding to the LIM< a steel plate is supported stably and efficiently. In this paper, after showing the advantages of the system, the magnetic levitation experiments are carried out to investigate the feasibility of the system.

  16. Soluble penicillin-binding protein 2a: beta-lactam binding and inhibition by non-beta-lactams using a 96-well format.

    PubMed

    Toney, J H; Hammond, G G; Leiting, B; Pryor, K D; Wu, J K; Cuca, G C; Pompliano, D L

    1998-01-01

    High level methicillin resistance in Staphylococcus aureus is dependent upon the acquisition of the mecA gene encoding penicillin-binding protein 2a (PBP2a). PBP2a is a member of a family of peptidoglycan biosynthetic enzymes involved in assembly of the cell wall in bacteria and is poorly inactivated by beta-lactam antibiotics. We describe a 96-well-filter binding assay using recombinant, soluble PBP2a which allows for kinetic measurement of penicillin binding. The deacylation rate constant for the PBP2a-penicillin G covalent complex was found to be 5.7 +/- 1.0 x 10(-5) s-1 at 30 degrees C (half-life of approximately 200 min). For the PBP2a acylation reaction, the value of K(m) (penicillin G) = 0.5 +/- 0.1 mM and kcat = 1 x 10(-3) s-1, which yields a second-order rate constant (kcat/K(m)) for inactivation of 2.0 M-1 s-1. Using this assay, several non-beta-lactam inhibitors including Cibacron blue have been found which exhibit IC50 values between 10 and 30 microM. The binding affinities of several carbapenems and beta-lactams correlated well between the filter binding assay described in this report and an electrophoretic assay for PBP2a using membranes prepared form methicillin-resistant S. aureus.

  17. An analytic model of convection in a system with layered viscosity and plates

    NASA Astrophysics Data System (ADS)

    Crowley, John W.; O'Connell, Richard J.

    2012-01-01

    We present an analytic boundary layer model for thermal convection with a finite-strength plate and depth-dependent viscosity. The model permits solutions in which convective flow rates in the mantle exceed the plate velocity. The energy balance equations for the lithosphere and convective cell are distinct and the model yields the plate velocity, plate thickness and heat flow, as well as the laterally averaged horizontal flow profile for a convective cell. We demonstrate, by inspection of the lithospheric energy balance, that the dominant plate driving force depends not only on the material properties of the mantle and lithosphere, but also on the velocity of the plate. Multiple solutions are possible with three solution branches for the plate velocity representing three distinct modes of thermal convection. The branch of solutions with the largest plate velocity corresponds to the classic boundary layer solution, with a plate velocity approximately equal to the maximum mantle velocity. This branch reproduces the classic convective scaling laws for an isoviscous fluid and its dynamics are controlled by the mantle material properties. The branch of solutions with intermediate plate velocity represents a convective cell with a sluggish-lid and a plate velocity that is less than the underlying mantle velocity. The dynamics for this solution branch depend on the material properties of both the mantle and the lithosphere. Finally, the lower branch of solutions for the plate velocity yields a convective solution in which the plate velocity is determined entirely by the local dynamics of a thick and strong (relative to the mantle) lithosphere. The dynamics of this solution branch are independent of the material properties of the mantle and depend entirely on the properties of the lithosphere. The introduction of an asthenosphere or low viscosity layer (LVL) beneath the plate can significantly alter the dynamics of the system by affecting plate-mantle coupling. Lowering

  18. Relative motions of the Australian, Pacific and Antarctic plates estimated by the Global Positioning System

    NASA Technical Reports Server (NTRS)

    Larson, Kristine M.; Freymueller, Jeff

    1995-01-01

    Global Positioning System (GPS) measurements spanning approximately 3 years have been used to determine velocities for 7 sites on the Australian, Pacific and Antarctic plates. The site velocities agree with both plate model predictions and other space geodetic techniques. We find no evidence for internal deformation of the interior of the Australian plate. Wellington, New Zealand, located in the Australian-Pacific plate boundary zone, moves 20 +/- 5 mm/yr west-southwest relative to the Australian plate. Its velocity lies midway between the predicted velocities of the two plates. Relative Euler vectors for the Australia-Antarctica and Pacific-Antarctica plates agree within one standard deviation with the NUVEL-1A predictions.

  19. Development and Clinical Evaluation of MatrixMANDIBLE Subcondylar Plates System (Synthes).

    PubMed

    Cortelazzi, Roberto; Altacera, Mario; Turco, Monica; Antonicelli, Viviana; De Benedittis, Michele

    2015-06-01

    In this article, authors report the different steps of development and clinical validation of MatrixMANDIBLE Subcondylar Plates (Synthes, Soletta, Switzerland), a specialized osteosynthesis system developed by Synthes during the past 4 years. Between 2009 and 2013, a total of 62 patients were treated for subcondylar and condylar neck fractures via a preauricular or retromandibular/transparotid approach. The MatrixMANDIBLE Subcondylar Plates System consists of a Trapezoidal Plate, a three-dimensional (3D) 4-hole 1.0-mm plate for smaller fracture areas, the Lambda Plate, a 7-hole 1.0-mm linear plate which mimics the two miniplates technique, and the Strut Plate, a 3D 1.0-mm plate with great versatility of employment. All devices satisfy the principles of a functionally stable osteosynthesis as stated by Champy et al. None of the plates broke and no macroscopic condylar displacement was noted on radiological follow-up. Clinical and functional parameters assessed at 6 months postoperative (mandibular range of motion, pain, dental occlusion) were almost restored. MatrixMANDIBLE Subcondylar Plates System (Synthes) has proved to provide sufficient mechanical stiffness and anatomically accurate fracture reduction to avoid major postoperative drawbacks of subcondylar and condylar neck fractures.

  20. Plate Motion and Crustal Deformation Estimated with Geodetic Data from the Global Positioning System

    NASA Technical Reports Server (NTRS)

    Argus, Donald F.; Heflin, Michael B.

    1995-01-01

    We use geodetic data taken over four years with the Global Positioning System (GPS) to estimate: (1) motion between six major plates and (2) motion relative to these plates of ten sites in plate boundary zones. The degree of consistency between geodetic velocities and rigid plates requires the (one-dimensional) standard errors in horizontal velocities to be approx. 2 mm/yr. Each of the 15 angular velocities describing motion between plate pairs that we estimate with GPS differs insignificantly from the corresponding angular velocity in global plate motion model NUVEL-1A, which averages motion over the past 3 m.y. The motion of the Pacific plate relative to both the Eurasian and North American plates is observed to be faster than predicted by NUVEL-1A, supporting the inference from Very Long B ase- line Interferometry (VLBI) that motion of the Pacific plate has speed up over the past few m.y. The Eurasia-North America pole of rotation is estimated to be north of NUVEL-1A, consistent with the independent hypothesis that the pole has recently migrated northward across northeast Asia to near the Lena River delta. Victoria, which lies above the main thrust at the Cascadia subduction zone, moves relative to the interior of the overriding plate at 30% of the velocity of the subducting plate, reinforcing the conclusion that the thrust there is locked beneath the continental shelf and slope.

  1. Method development for the quantitation of ABT-578 in rabbit artery tissue by 96-well liquid-liquid extraction and liquid chromatography/tandem mass spectrometric detection.

    PubMed

    Ji, Qin C; Zhang, Jun; Rodila, Ramona; Watson, Pamela; El-Shourbagy, Tawakol

    2004-01-01

    Quantitative determination of drug concentrations in tissue samples can provide critical information for drug metabolism, kinetics, and toxicity evaluations. For analysis of tissue samples using liquid chromatography/tandem mass spectrometric (LC/MS/MS) detection, homogenization is a critical step in achieving good assay performance. Assay performance can be closely evaluated by spiking the drug directly into tissue samples prior to homogenization. It is especially important to include this assay evaluation for the analysis of artery tissue samples because artery tissue is very elastic, making it quite a challenge to develop an effective procedure for homogenization. An LC/MS/MS assay in 96-well format using liquid-liquid extraction was developed for analyzing ABT-578 in rabbit artery samples. Tissue quality control samples were prepared by spiking ABT-578 stock solutions directly into the tissue before homogenization. The usage of the tissue control samples gives a thorough evaluation of the sample preparation process that includes both homogenization and sample extraction. A 20% blood in saline solution was used as a homogenization solution. Calibration standards were made by spiking ABT-578 into rabbit whole blood. Blood quality control samples were also prepared by spiking ABT-578 into rabbit whole blood. These blood QC samples were used to confirm the validity of the calibration curve. A lower limit of quantitation of 0.050 ng/mL was achieved. The linear dynamic range of blood standards was from 0.050-30.3 ng/mL with the correlation coefficient (r) ranging from 0.9969-0.9996. Overall %CV was between 1.3 and 7.0%, and analytical recovery was between 98.2 and 105.8% for blood QC samples. The %CVs for tissue QC samples were between 6.7 and 13.0%, and analytical recovery after correction was between 93.5 and 114.3%.

  2. A high-throughput protein refolding screen in 96-well format combined with design of experiments to optimize the refolding conditions.

    PubMed

    Dechavanne, Vincent; Barrillat, Nicolas; Borlat, Frederic; Hermant, Aurélie; Magnenat, Laurent; Paquet, Mikael; Antonsson, Bruno; Chevalet, Laurent

    2011-02-01

    Production of correctly folded and biologically active proteins in Escherichiacoli can be a challenging process. Frequently, proteins are recovered as insoluble inclusion bodies and need to be denatured and refolded into the correct structure. To address this, a refolding screening process based on a 96-well assay format supported by design of experiments (DOE) was developed for identification of optimal refolding conditions. After a first generic screen of 96 different refolding conditions the parameters that produced the best yield were further explored in a focused DOE-based screen. The refolding efficiency and the quality of the refolded protein were analyzed by RP-HPLC and SDS-PAGE. The results were analyzed by the DOE software to identify the optimal concentrations of the critical additives. The optimal refolding conditions suggested by DOE were verified in medium-scale refolding tests, which confirmed the reliability of the predictions. Finally, the refolded protein was purified and its biological activity was tested in vitro. The screen was applied for the refolding of Interleukin 17F (IL-17F), stromal-cell-derived factor-1 (SDF-1α/CXCL12), B cell-attracting chemokine 1 (BCA-1/CXCL13), granulocyte macrophage colony stimulating factor (GM-CSF) and the complement factor C5a. This procedure identified refolding conditions for all the tested proteins. For the proteins where refolding conditions were already available, the optimized conditions identified in the screening process increased the yields between 50% and 100%. Thus, the method described herein is a useful tool to determine the feasibility of refolding and to identify high-yield scalable refolding conditions optimized for each individual protein.

  3. Development, validation and application of a 96-well enzymatic assay based on LC-ESI-MS/MS quantification for the screening of selective inhibitors against Mycobacterium tuberculosis purine nucleoside phosphorylase.

    PubMed

    Cattaneo, Giulia; Ubiali, Daniela; Calleri, Enrica; Rabuffetti, Marco; Höfner, Georg C; Wanner, Klaus T; De Moraes, Marcela C; Martinelli, Leonardo K B; Santos, Diógenes Santiago; Speranza, Giovanna; Massolini, Gabriella

    2016-11-02

    Mycobacterium tuberculosis (Mtb) purine nucleoside phosphorylase (PNP, EC 2.4.2.1) has been identified as a target for the development of specific inhibitors with potential antimycobacterial activity. We hereby described the development and validation of a new 96-well LC-ESI-MS/MS method to assess the inhibition activity of nucleoside analogues towards MtbPNP and the human PNP (HsPNP). Enzyme activity was determined by monitoring the phosphorolysis of inosine (Ino) to hypoxanthine (Hpx). The enzymatic assay (v = 0.5 mL, enzyme<0.2 μg/well, T = 37 °C) was performed with an overall time of about 15 min/plate for sample processing and 2 min/sample for LC-MS analysis. Validation of the quantification method met the criteria of the CDER guidance of FDA. Kinetic parameters were in agreement with those reported in literature (HsPNP KM = 0.150 ± 0.020 mM vs 0.133 ± 0.015 mM; MtbPNP KM = 0.060 ± 0.009 mM vs 0.040 ± 0.003 mM for Ino), thus demonstrating the reliability of the newly developed enzymatic assay. Preliminary inhibition assays confirmed the effects reported for Acyclovir (Acv) and Formycin A (FA) against HsPNP and MtbPNP. The validated enzymatic assay was applied to the evaluation of a set of 8-halo-, 8-amino-, 8-O-alkyl-substituted purine ribonucleosides synthesized on purpose as potential inhibitors against MtbPNP. The assayed 8-substituted ribonucleosides did not exert a significant inhibitory effect against the tested enzymes up to 1 mM.

  4. Risk factors for breakage of biodegradable plate systems after bilateral sagittal split mandibular setback surgery.

    PubMed

    Yoshioka, Izumi; Igawa, Kaori; Nagata, Jyunko; Yoshida, Maho; Baba, Takashi; Ichiki, Takeshi; Kondoh, Yudai; Takamori, Koichi; Kashima, Koji; Sakoda, Sumio

    2013-06-01

    The aim of this retrospective study was to evaluate the risk factors associated with breakage of biodegradable plate systems after bilateral sagittal split mandibular setback. We studied 169 Japanese adults (62 men, 107 women; age range 16-53 years) with deformities of the jaw diagnosed as mandibular prognathism. All patients were treated by bilateral sagittal split osteotomy (BSSO) with 2 biodegradable fixation plates and screws at the anterior mandibular ramus. We collected the following data from the medical records and radiological findings: sex; age; degree of setback; presence of asymmetry; presence of open bite; operation; design of the plate; operating time; and blood loss. Multiple logistic regression analysis was used to find the factors that were independently associated with the dependent variable: breakage of the biodegradable plate system. In 10 of the 169 patients (6%) the biodegradable plate system for the BSSO broke. Factors that influenced whether or not the biodegradable plate system fractured were if they were asymmetrical (odds ratio (OR) 5.35; P=0.02) and had an open bite (OR 5.20; P=0.02). Asymmetry or open bite was significantly associated with breaks in the biodegradable plate system. Biodegradable plates should be used only when loading is minimal.

  5. The Demonstrator for the European Plate Observing System (EPOS)

    NASA Astrophysics Data System (ADS)

    Hoffmann, T. L.; Euteneuer, F.; Ulbricht, D.; Lauterjung, J.; Bailo, D.; Jeffery, K. G.

    2014-12-01

    An important outcome of the 4-year Preparatory Phase of the ESFRI project European Plate Observing System (EPOS) was the development and first implementation of the EPOS Demonstrator by the project's ICT Working Group 7. The Demonstrator implements the vertical integration of the three-layer architectural scheme for EPOS, connecting the Integrated Core Services (ICS), Thematic Core Services (TCS) and the National Research Infrastructures (NRI). The demonstrator provides a single GUI with central key discovery and query functionalities, based on already existing services by the seismic, geologic and geodetic communities. More specifically the seismic services of the Demonstrator utilize webservices and APIs for data and discovery of raw seismic data (FDSN webservices by the EIDA Network), events (Geoportal by EMSC) and analytical data products (e.g., hazard maps by EFEHR via OGC WMS). For geologic services, the EPOS Demonstrator accesses OneGeology Europe which serves the community with geologic maps and point information via OGC webservices. The Demonstrator also provides access to raw geodetic data via a newly developed universal tool called GSAC. The Demonstrator itself resembles the future Integrated Core Service (ICS) and provides direct access to the end user. Its core functionality lies in a metadata catalogue, which serves as the central information hub and stores information about all RIs, related persons, projects, financial background and technical access information. The database schema of the catalogue is based on CERIF, which has been slightly adapted. Currently, the portal provides basic query functions as well as cross domain search. [www.epos.cineca.it

  6. A radiophotoluminescent glass plate system for medium-sized field dosimetry

    SciTech Connect

    Nakagawa, Keiichi; Koyanagi, Hiroki; Shiraki, Takashi; Saegusa, Shigeki; Sasaki, Katsutake; Oritate, Takashi; Mima, Kazuo; Miyazawa, Masanori; Ishidoya, Tatsuyo; Ohtomo, Kuni; Yoda, Kiyoshi

    2005-10-15

    A two-dimensional radiophotoluminescent system for medium-sized field dosimetry has been developed using a silver-activated phosphate glass plate with a dimension of 120 mmx120 mmx1 mm and a readout unit comprising a UV excitation lamp and a CCD imager. A dose ranging from 0 to 400 cGy, provided by a 6 MV x-ray beam, was delivered to the glass plate oriented perpendicularly to the beam and positioned in a water phantom at a depth of 10 cm, where the center of the glass plate coincided with the linac isocenter. After the dose delivery, the glass plate was placed in the readout system. The CCD output intensity increased linearly with the applied dose. The angular dependence of response on the direction of radiation incidence was measured by rotating the glass plate in the water phantom, indicating that the output remained constant up to 75 deg. from perpendicular incident direction, followed by a steep reduction down to 85% at an angle of 90 deg. A lateral dose distribution resulting from a 60 mmx60 mm irradiation was compared between the glass plate and an x-ray film having had the same exposure, showing that the glass plate and the x-ray film led to identical dose distributions. The dose reproducibility for a glass plate and the sensitivity variation among different glass plates were also evaluated.

  7. Multi-well sample plate cover penetration system

    DOEpatents

    Beer, Neil Reginald [Pleasanton, CA

    2011-12-27

    An apparatus for penetrating a cover over a multi-well sample plate containing at least one individual sample well includes a cutting head, a cutter extending from the cutting head, and a robot. The cutting head is connected to the robot wherein the robot moves the cutting head and cutter so that the cutter penetrates the cover over the multi-well sample plate providing access to the individual sample well. When the cutting head is moved downward the foil is pierced by the cutter that splits, opens, and folds the foil inward toward the well. The well is then open for sample aspiration but has been protected from cross contamination.

  8. Research and implementation of the integrated cooling system for focal plate

    NASA Astrophysics Data System (ADS)

    Wang, Jianping; Cheng, Lixuan; Chu, Jiaru; Hu, Hongzhuan; Zhou, Zengxiang

    2016-07-01

    With the rapid development of multi-objective astronomical survey telescope technology, the heat of focal plate which high-density optical fiber positioners were mounted in has become the key factor of system precision. The new integrated cooling system designed multi curved composite grooves on the surface of focal plate for forced convection was proposed. Meanwhile, the manufacturing process, sealing structure and heat dissipation performance of the system were analyzed and tested with detail in the paper. The experimental results suggested that the new integrated cooling system of focal plate has a fast response speed and good heat dissipation performance.

  9. High-throughput, semi-automated determination of a cyclooxygenase II inhibitor in human plasma and urine using solid-phase extraction in the 96-well format and high-performance liquid chromatography with post-column photochemical derivatization-fluorescence detection.

    PubMed

    Matthews, C Z; Woolf, E J; Lin, L; Fang, W; Hsieh, J; Ha, S; Simpson, R; Matuszewski, B K

    2001-02-25

    Compound I, 5-chloro-3-(4-methanesulfonylphenyl)-6'-methyl-[2,3']bipyridinyl, has been found to be a specific inhibitor of the enzyme cyclooxygenase II (COX II). The anti-inflammatory properties of this compound are currently being investigated. HPLC assays for the determination of this analyte in human plasma and human urine have been developed. Isolation of I and the internal standard (II) was achieved by solid-phase extraction (SPE) in the 96-well format. A C8 SPE plate was used for the extraction of the drug from human plasma (recovery >90%) while a mixed-mode (C8/Cation) SPE plate was used to isolate the analytes from human urine (recovery approximately 71%). The analyte and internal standard were chromatographed on a Keystone Scientific Prism-RP guard column (20 x 4.6 mm) connected to a Prism-RP analytical column (150 x 4.6 mm), using a mobile phase consisting of 45% acetonitrile in 10 mM acetate buffer (pH = 4); the analytes eluted at retention times of 5.2 and 6.9 min for I and II, respectively. Compounds I and II were found to form highly fluorescent products after exposure to UV light (254 nm). Thus, the analytes were detected by fluorescence (lambda(ex) = 260 nm, lambda(em) =375 nm) following post-column photochemical derivatization. Eight point calibration curves over the concentration range of 5-500 ng/ml for human plasma and human urine yielded a linear response (R2>0.99) when a 1/y weighted linear regression model was employed. Based on the replicate analyses (n = 5) of spiked standards, the within-day precision for both assays was better than 7% C.V. at all points on the calibration curve; within-day accuracy was within 5% of nominal at all standard concentrations. The between-run precision and accuracy of the assays, as calculated from the results of the analysis of quality control samples, was better than 8% C.V. and within 8% of nominal. I was found to be stable in human plasma and urine for at least 8 and 2 months, respectively. In addition, the

  10. The Core Services of the European Plate Observing System (EPOS)

    NASA Astrophysics Data System (ADS)

    Hoffmann, T. L.; Euteneuer, F. H.; Lauterjung, J.

    2013-12-01

    The ESFRI project European Plate Observing System (EPOS) was launched in November 2010 and has now completed its year 3 of the four-year preparatory phase. EPOS will create a single sustainable, permanent observation infrastructure, integrating existing geophysical monitoring networks, local observatories and experimental laboratories in Europe and adjacent regions. EPOS' technical Work Package 6 has developed a three layer architectural model for the construction of the EPOS Core Services (CS) during the subsequent implementation phase. The Poster will present and detail on these three layers, consisting of the EPOS Integrated Core Services (ICS), the Thematic Core Services (TCS) and the existing National Research Infrastructures & Data Centers. The basic layer of the architecture is established by the National Research Infrastructures (RIs) & Data Centers, which generate data and information and are responsible for the operation of the instrumentation. National RIs will provide their data to the Thematic Cores Services. The Thematic Core Services constitute the community layer of EPOS architecture and they will: 1) consist of existing (e.g. ORFEUS, EMSC), developing (e.g. EUREF/GNSS) or still to be developed Service Providers for specific thematic communities, as represented within EPOS through the technical EPOS Working Groups (e.g., seismology, volcanology, geodesy, geology, analytic labs for rock physics, geomagnetism, geo-resources ... and many others), 2) provide data services to specific communities, 3) link the National Research Infrastructures to the EPOS Integrated Services, 4) include Service Providers (e.g. OneGeology+, Intermagnet) that may be merely linked or partially integrated and 5) consist of Integrated Laboratories and RIs spanning multiple EPOS disciplines and taking advantage of other existing Thematic Services. The EPOS Integrated Services constitute the ICT layer of the EPOS portal and they will: 1) provide access to multidisciplinary data

  11. Plate Tectonics as a Far-From-Equilibrium Self-Organized Dissipative System

    NASA Astrophysics Data System (ADS)

    Anderson, D. L.

    2001-12-01

    A fluid above the critical Rayleigh number is far from equilibrium and spontaneously organizes itself into patterns involving the collective motion of large numbers of molecules which are resisted by the viscosity of the fluid. No external template is involved in forming the pattern. In 1928 Pearson showed that Bénard's experiments were driven by variations in surface tension at the top of the fluid and the surface motions drove convection in the fluid. In this case, the surface organized itself AND the underlying fluid. Both internal buoyancy driven flow and flow driven by surface forces can be far-from-equilibrium self-organized open systems that receive energy and matter from the environment. In the Earth, the cold thermal boundary layer at the surface drives plate tectonics and introduces temperature, shear and pressure gradients into the mantle that drive mantle convection. The mantle provides energy and material but may not provide the template. Plate tectonics is therefore a candidate for a far-from-equilibrium dissipative self-organizing system. Alternatively, one could view mantle convection as the self-organized system and the plates as simply the surface manifestation. Lithospheric architecture also imposes lateral temperature gradients onto the mantle which can drive and organize flow. Far-from-equilibrium self-organization requires; an open system, interacting parts, nonlinearities or feedbacks, an outside steady source of energy or matter, multiple possible states and a source of dissipation. In uniform fluids viscosity is the source of dissipation. Sources of dissipation in the plate system include bending, breaking, folding, shearing, tearing, collision and basal drag. These can change rapidly, in contrast to plate driving forces, and introduce the sort of fluctuations that can reorganize far-from-equilibrium systems. Global plate reorganizations can alternatively be thought of as convective overturns of the mantle, or thermal weakening of plates

  12. Design and Development of ZigBee Based Instantaneous Flat-plate Collector Efficiency Measurement System

    NASA Astrophysics Data System (ADS)

    Vairamani, K.; Venkatesh, K. Arun; Mathivanan, N.

    2011-01-01

    Computing the efficiency of flat-plate collector is vital in solar thermal system testing. This paper presents the design of ZigBee enabled data acquisition system for instantaneous flat-plate collector efficiency calculation. It involves measurement of parameters like inlet and outlet fluid temperatures, ambient temperature and solar radiation intensity. The designed system has a base station and a sensor node. ZigBee wireless communication protocol is used for communication between the base station and the sensor node for wireless data acquisition. The wireless sensor node which is mounted over the collector plate includes the necessary sensors and associated signal-conditioners. An application program has been developed on LabVIEW platform for data acquisition, processing and analysis and is executed in base station PC. Instantaneous flat-plate collector efficiency is computed and reported.

  13. Optimization of 384-well Luminex Immunoassays on the FlexMAP 3D System for Routine Analyte Quantitation from Commercial Kits

    PubMed Central

    Pergande, Melissa; Pithadia, Ravi; Borgia, Jeffrey A.; Fhied, Cristina

    2014-01-01

    INTRODUCTION: The Luminex immunobead platform offers the benefits of ELISA-based assays, but also permits higher throughput, increased flexibility, reduced sample volume, and lower cost when evaluation of multiple analytes is necessary. The FlexMap 3D system is a compact, high bead throughput instrument which can read up to 500 different bead regions from a single sample in a 96 or 384-well plate format. Commercially-available kits for the measurement of protein analytes are currently only available in the 96-well plate format. The objective of this study was to adapt and validate the commonly used 96-well plate protocols to the 384-well plate format with equivalent or improved test performance characteristics. METHODS: A laboratory-developed 96-well plate assay protocol for Vimentin autoantibody quantitation was translated and optimized for a 384-well plate format. Assay sensitivity was evaluated by comparing the results from two commercially available kits (Human IGF Binding Proteins 1-7 and Human IGF I/II), first employing the traditional 96-well plate format and subsequently using the newly optimized 384-well plate format. RESULTS: Conversion from the 96 to 384-well plate format for our Vimentin autoantibody assay revealed comparable sensitivity, while consuming only about a quarter of the reagents and specimen. Results from the IGF-I assay revealed approximately equivalent sensitivity (±2.4%), whereas IGF-II showed a significant increase in sensitivity (17.7%), both when the 96 and 384-well plate formats were ran concomitantly. No significant alterations in assay range, recovery or precision were otherwise noted. CONCLUSION: In this study, we have demonstrated that the conversion of a 96 to 384-well plate format was developed and validated successfully. When employing our optimized 384-well plate format, we found that nearly 4 times as many samples can be ran additionally with one commercially available kit, saving in not only cost but also sample size.

  14. Plate kinematics of the Afro-Arabian Rift System with emphasis on the Afar Depression, Ethiopia

    NASA Astrophysics Data System (ADS)

    Bottenberg, Helen Carrie

    This work utilizes the Four-Dimensional Plates (4DPlates) software, and Differential Interferometric Synthetic Aperture Radar (DInSAR) to examine plate-scale, regional-scale and local-scale kinematics of the Afro-Arabian Rift System with emphasis on the Afar Depression in Ethiopia. First, the 4DPlates is used to restore the Red Sea, the Gulf of Aden, the Afar Depression and the Main Ethiopian Rift to development of a new model that adopts two poles of rotation for Arabia. Second, the 4DPlates is used to model regional-scale and local-scale kinematics within the Afar Depression. Most plate reconstruction models of the Afro-Arabian Rift System relies on considering the Afar Depression as a typical rift-rift-rift triple junction where the Arabian, Somali and Nubian (African) plates are separating by the Red Sea, the Gulf of Aden and the Main Ethiopian Rift suggesting the presence of "sharp and rigid" plate boundaries. However, at the regional-scale the Afar kinematics are more complex due to stepping of the Red Sea propagator and the Gulf of Aden propagator onto Afar as well as the presence of the Danakil, Ali Sabieh and East Central Block "micro-plates". This study incorporates the motion of these micro-plates into the regional-scale model and defined the plate boundary between the Arabian and the African plates within Afar as likely a diffused zone of extensional strain within the East Central Block. Third, DInSAR technology is used to create ascending and descending differential interferograms from the Envisat Advanced Synthetic Aperture Radar (ASAR) C-Band data for the East Central Block to image active crustal deformation related to extensional tectonics and volcanism. Results of the DInSAR study indicate no strong strain localization but rather a diffused pattern of deformation across the entire East Central Block.

  15. Biomechanical investigation of two plating systems for medial column fusion in foot

    PubMed Central

    Simons, Paul; Sommerer, Theresia; Zderic, Ivan; Wahl, Dieter; Lenz, Mark; Skulev, Hristo; Knobe, Matthias; Gueorguiev, Boyko; Richards, R. Geoff; Klos, Kajetan

    2017-01-01

    Background Arthrodesis of the medial column (navicular, cuneiform I and metatarsal I) is performed for reasons such as Charcot arthropathy, arthritis, posttraumatic reconstruction or severe pes planus. However, the complication rate is still high and mainly resulting from inadequate fixation. Special plates, designed for medial column arthrodesis, seem to offer potential to reduce the complication rate. The aim of this study was to investigate biomechanically plantar and dorsomedial fusion of the medial column using two new plating systems. Methods Eight matched pairs of human cadaveric lower legs were randomized in two groups and medial column fusion was performed using either plantar or dorsomedial variable-angle locking compression plates. The specimens were biomechanically tested under cyclic progressively increasing axial loading with physiological profile of each cycle. In addition to the machine data, mediolateral x-rays were taken every 250 cycles and motion tracking was performed to determine movements at the arthrodesis site. Statistical analysis of the parameters of interest was performed at a level of significance p = 0.05. Results Displacement of the talo-navicular joint after 1000, 2000 and 4000 cycles was significantly lower for plantar plating (p≤0.039) while there was significantly less movement in the naviculo-cuneiform I joint for dorsal plating post these cycle numbers (p<0.001). Displacements in all three joints of the medial column, as well as angular and torsional deformations between the navicular and metatarsal I increased significantly for each plating technique between 1000, 2000 and 4000 cycles (p≤0.021). The two plating systems did not differ significantly with regard to stiffness and cycles to failure (p≥0.171). Conclusion From biomechanical point of view, although dorsomedial plating showed less movement than plantar plating in the current setup under dynamic loading, there was no significant difference between the two plating

  16. Lengthening over nails using the double plate system ONAS-DPS

    PubMed Central

    Antolič, Vane

    2016-01-01

    Stable insertion of large Schanz screws behind an intramedullary (IM) nail when lengthening over nails (LON) may be difficult due to the limited bone stock. Additionally, the highly probable contact between the screws and IM nail (which is difficult to avoid) increases the likelihood of infection spreading from the skin via Schanz screws directly to the IM nail. A new device for LON has been developed. Instead of inserting Schanz screws from the external fixator beside the IM nail (as in standard LON), a system of two overlaying plates was constructed. Schanz screws can be fixed to the plates without entering the bone. The plates are fixed to the bone using four angle stability screws. The holes in the plates offer stabile fixation for a chosen angle under which the screw is positioned through the cortical bone. Using the new system there is no need to place Schanz screws behind the IM nail. Instead, Schanz screws pass to the plate and not through the bone. The new system for elongation over IM nail is called “Over Nail Angle Stability-Double Plate System” (ONAS-DPS) [Antolič V (2013) Modular side device with an intramedullary nail for guiding a bone during its lengthening. World Intellectual Property Organization. International Publication number: WO 2013/176632 A1]. PMID:27163094

  17. Evaluation of poly(lactic-co-glycolic acid) plate and screw system for bone fixation.

    PubMed

    Park, Subin; Kim, Jin Hee; Kim, Il Hwan; Lee, Minsu; Heo, Suhak; Kim, Hong; Kim, Eun Hee; Choy, Young Bin; Heo, Chan Yeong

    2013-05-01

    In this study, we investigated the efficacy and safety of the recently developed modifiable bioabsorbable plates and screws, which are made of PLGA [poly(lactic-co-glycolic acids)]. An in vitro extract test and a mammalian erythrocyte micronucleus test revealed that neither cytotoxicity nor genotoxicity was observed with the plates and screws tested in this study. An in vivo mandible fracture model in rabbit was introduced to evaluate the in vivo efficacy and of the PLGA-based plates and screws. At 4, 6, 8, and 10 weeks after implantation, tissue specimens were taken from the implanted sites of the rabbits and a histologic analysis was performed for each of the specimens. After 4 weeks, the plate was covered by connective tissues and severe chronic active inflammation in soft tissue was observed. After 6 weeks, the inflammation decreased and some of the specimens exhibited new bone formation around the periosteum. After 8 and 10 weeks, new bone formation was observed with all samples, where almost no severe inflammation was involved, implying the healing of the fracture. Given these, it can be suggested that the biodegradable plate and screw system that we evaluated in this study is effective for treatment of mandible fracture, one of the regions under a high load-bearing condition. The adjustment process and the long-term follow-up study are in progress for clinical application of the plate and screw system introduced in this study.

  18. A force plate system for measuring low-magnitude reaction forces in small laboratory animals.

    PubMed

    Handley, D E; Ross, J F; Carr, G J

    1998-07-01

    We present a force plate system which measures low-magnitude vertical reaction forces generated by small laboratory animals. The force plate mechanical design minimizes radiated transverse waves, acoustic reverberation, and standing waves caused by impacts on the force plate surface. A secondary force plate and PC-based software algorithm minimize floor vibrational artifact. The force plate was used to measure function of rats during two tests: forelimb/hindlimb hopping reaction and surface righting reaction. In control rats, forelimb hopping rate exceeded hindlimb hopping rate during 16 weeks of repeated testing. Subchronic intraperitoneal (i.p.) dosing of 10 mg/kg/day acrylamide produced a selective impairment of hindlimb hopping. In contrast, single doses of haloperidol (1-5 mg/kg, i.p.) slowed the righting reaction and produced a relatively selective impairment of forelimb hopping. The force plate system presents new opportunities for performing quantitative neurological assessments of small laboratory animals when previously such tests had been performed subjectively and qualitatively.

  19. A novel combined lift and propulsion system for a steel plate conveyance by electromagnets

    SciTech Connect

    Hayashiya, H.; Iizuka, D.; Ohsaki, H.; Masada, E.

    1998-07-01

    In the steel making process, there is a requirement to improve the quality of the products. To realize such a requirement, non-contacting conveyance of a steel plate by magnetic force has been investigated. The advantages brought by introducing non-contacting conveyance of a steel plate are improved quality of products, reduced maintenance cost of installations, increased productivity, and quieter operation. Here, a novel lift and propulsion system using alternating-current electromagnets is proposed. The proposed system can simplify the configuration of a non-contacting conveyance system for a steel plate by magnetic force. Both finite element analysis and experiments were carried out. After investigating the basic characteristics of the proposed electromagnetic linear actuator, magnetic levitation experiments with four controlled electromagnets were performed and stable levitation was realized though about 65% of the total mass of the steel plate was supported by uncontrolled alternating-current electromagnets. The results indicate that the proposed system can produce not only the strong levitation force but also the propulsion force on a steel plate.

  20. Oscillations of a spring-magnet system damped by a conductive plate

    NASA Astrophysics Data System (ADS)

    Ladera, C. L.; Donoso, G.

    2013-09-01

    We study the motion of a spring-magnet system that oscillates with very low frequencies above a circular horizontal non-magnetizable conductive plate. The magnet oscillations couple with the plate via the Foucault currents induced therein. We develop a simple theoretical model for this magneto-mechanical oscillator, a model that leads to the equation of a damped harmonic oscillator, whose weak attenuation constant depends upon the system parameters, e.g. the electrical conductivity of the constituent material of the plate and its thickness. We present a set of validating experiments, the results of which are predicted with good accuracy by our analytical model. Additional experiments can be performed with this oscillating system or its variants. This oscillator is simple and low-cost, easy to assemble, and can be used in experiments or project works in physics teaching laboratories at the undergraduate level.

  1. Proceedings of the Flat-Plate Solar Array Project Research Forum on Photovoltaic Metallization Systems

    NASA Technical Reports Server (NTRS)

    1983-01-01

    A photovoltaic Metallization Research forum, under the sponsorship of the Flat-Plate Solar Array Project consisted of five sessions, covering: (1) the current status of metallization systems, (2) system design, (3) thick-film metallization, (4) advanced techniques, and (5) future metallization challenges.

  2. 76 FR 60531 - National Institute of Justice Interview Room Recording Systems and License Plate Readers Workshop

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-29

    ... Office of Justice Programs National Institute of Justice Interview Room Recording Systems and License... Recording Systems and License Plate Readers Workshops. SUMMARY: The National Institute of Justice (NIJ) and... the 118th Annual IACP Conference in Chicago, Ill. The focus of the workshops is the development of...

  3. Design of Stress-Strain Measuring System for Bulldozing Plate Based on Virtual Instrument Technology

    NASA Astrophysics Data System (ADS)

    Xu, S. C.; Li, J. Q.; Zhang, R.

    2006-10-01

    Soil is a kind of discrete, multiphase compound that is composed of soil particles, liquid and air. When soil is disturbed by bulldozing plate, the mechanical behavior of the soil will become very complex. Based on the law of action and reaction, the dynamic mechanical behavior of disturbed soil was indirectly analyzed by measuring and studying the forces on the bulldozing plate by soil currently, so a stress-strain virtual measuring system for bulldozing plate, which was designed by the graphical programming language DASYLab, was used to measure the horizontal force Fz acting on the bulldozing plate. In addition, during the course of design, the experimental complexities and the interferential factors influencing on signal logging were analyzed when bulldozing plate worked, so the anti-jamming methods of hardware and software technology were adopted correlatively. In the end, the horizontal force Fz was analyzed with Error Theory, the result shown that the quantificational analysis of Fz were identical to the qualitative results of soil well, and the error of the whole test system is under 5 percent, so the tress-strain virtual measuring system was stable and credible.

  4. Development of walking analysis system consisting of mobile force plate and motion sensor.

    PubMed

    Adachi, Wataru; Tsujiuchi, Nobutaka; Koizumi, Takayuki; Aikawa, Masataka; Shiojima, Kouzou; Tsuchiya, Youtaro; Inoue, Yoshio

    2011-01-01

    In walking analysis, which is one useful method for efficient physical rehabilitation, the ground reaction force, the center of pressure, and the body orientation data are measured during walking. In the past, these data were measured by a 3D motion analysis system consisting of high-speed cameras and force plates, which must be installed in the floor. However, a conventional 3D motion analysis system can measure the ground reaction force and the center of pressure just on force plates during a few steps. In addition, the subjects' stride lengths are limited because they have to walk on the center of the force plate. These problems can be resolved by converting conventional devices into wearable devices. We used a measuring device consisting of portable force plates and motion sensors. We developed a walking analysis system that calculates the ground reaction force, the center of pressure, and the body orientations and measured a walking subject to estimate this system. We simultaneously used a conventional 3D motion analysis system to compare with our development system and showed its validity for measurements of ground reaction force and the center of pressure.

  5. An automatic system for measurement of retardation of wave plates based on phase-shifted method

    NASA Astrophysics Data System (ADS)

    Gao, Zhishan; Yan, Ming

    2005-02-01

    A practical system is described to measure the retardation of wave plates with phase-shifted method. The tested wave plate is put in and the original angle between the axis of it and the analyzer is random, not 45 degree. For the measurement is made rapidly and automatically, a standard wave plate act as a compensator, the stepping motor is used to drive the analyzer to realize phase shifting and a grating encoder is used to measure its rotating angle. At the same time, while the beam comes out from the analyzer, the photoelectric detector gets its intensity, and then the signals is magnified, filtered and sent to computer through its serial port. The results show the system has the advantages of costing little time and high accuracy.

  6. Novel 96-well quantitative bioelectrocatalytic analysis platform reveals highly efficient direct electrode regeneration of cytochrome P450 BM3 on indium tin oxide.

    PubMed

    Frank, Ronny; Klenner, Marcus; Azendorf, Ronny; Bartz, Manuel; Jahnke, Heinz-Georg; Robitzki, Andrea A

    2016-08-18

    Enzymes are the most effective catalysts for a broad range of difficult chemical reactions e.g. hydroxylation of non-activated C-H Bonds and stereoselective synthesis. Nevertheless, a lot of enzymes are not accessible for the biotechnological applications or industrial use. One reason is the prerequisite of expensive cofactors. In this context, we developed a bioelectrocatalytic analysis platform for the electrochemical and photonic quantification of the direct electron transfer from the electrode to redox enzymes and therefore, bypass the need of soluble cofactors that had to be continuously exchanged or regenerated. As reference enzyme, we chose cytochrome P450 BM3 that is restricted by NADPH dependence. We optimized the substrate spectrum for aromatic compounds by introduction of the triple mutation A74G/F87V/L188Q and established a sensitive fluorimetric product formation assay to monitor the enzymatic conversion of 7-ethoxycoumarine to 7-hydroxycoumarine. Gold and indium tin oxide electrodes were characterized with respect to surface morphology, charge-transfer resistance and P450 BM3 immobilization as well as activity. Using gold electrodes, no significant product formation by electrode mediated direct electron transfer could be detected. In contrast, P450 BM3 adsorbed on unmodified indium tin oxide electrodes revealed 36% activity by electrode mediated direct electron transfer in comparison to enzyme regeneration by NADPH. Since the reaction volumes are in the microliter range and upscaling of the measurement system is easily possible, our analysis platform is a useful tool for bioelectrocatalytic enzyme characterization and library screening based optimization for applications in the field of enzyme catalyzed chemical synthesis but also enzyme based fuel cells.

  7. VAPI: low-cost, rapid automated visual inspection system for Petri plate analysis

    NASA Astrophysics Data System (ADS)

    Chatburn, L. T.; Kirkup, B. C.; Polz, M. F.

    2007-09-01

    Most culture-based microbiology tasks utilize a petri plate during processing, but rarely do the scientists capture the full information available from the plate. In particular, visual analysis of plates is an under-developed rich source of data that can be rapid and non-invasive. However, collecting this data has been limited by the difficulties of standardizing and quantifying human observations, by the limits of a scientists' fatigue, and by the cost of automating the process. The availability of specialized counting equipment and intelligent camera systems has not changed this - they are prohibitively expensive for many laboratories, only process a limited number of plate types, are often destructive to the sample, and have limited accuracy. This paper describes an automated visual inspection solution, VAPI, that employs inexpensive consumer computing hardware and digital cameras along with custom cross-platform open-source software written in C++, combining Trolltech's Qt GUI toolkit with Intel's OpenCV computer vision library. The system is more accurate than common commercial systems costing many times as much, while being flexible in use and offering comparable responsiveness. VAPI not only counts colonies but also sorts and enumerates colonies by morphology, tracks colony growth by time series analysis, and provides other analytical resources. Output to XML files or directly to a database provides data that can be easily maintained and manipulated by the end user, offering ready access for system enhancement, interaction with other software systems, and rapid development of advanced analysis applications.

  8. Side-by-side comparisons of evacuated compound parabolic concentrator and flat plate solar collector systems

    SciTech Connect

    McGarity, A.E.; Allen, J.W.; Schertz, W.W.

    1983-10-01

    Three liquid-based solar heating systems employing different types of solar collectors were tested side by side near Chicago, Illinois for one year. The three different types of collectors were: a flat plate collector with a black-chrome coated absorber plate and one low-iron glass cover; an evacuated-tube compound parabolic concentrator (CPC) with a concentration ratio of 1.1, oriented with tubes and troughs along a north-south axis; and an evacuated-tube CPC collector with a concentration ratio of 1.3 and one low-iron glass cover, with tubes and troughs oriented along an east-west axis. Results indicate that the flat plate collector system was the most efficient during warm weather, but the CPC systems were more efficient during cold weather, but the CPC systems were more efficient during cold weather, and the CPC systems operated under conditions too adverse for the flat plate collector. The computer simulation model ANSIM was validated by means of the side-by-side tests. The model uses analytical solutions to the storage energy balance. ANSIM is compared with the general simulation TRNSYS. (LEW)

  9. Flate-plate photovoltaic power systems handbook for Federal agencies

    NASA Technical Reports Server (NTRS)

    Cochrane, E. H.; Lawson, A. C.; Savage, C. H.

    1984-01-01

    The primary purpose is to provide a tool for personnel in Federal agencies to evaluate the viability of potential photovoltaic applications. A second objective is to provide descriptions of various photovoltaic systems installed by different Federal agencies under the Federal Photovoltaic Utilization Program so that other agencies may consider similar applications. A third objective is to share lessons learned to enable more effective procurement, design, installation, and operation of future photovoltaic systems. The intent is not to provide a complete handbook, but rather to provide a guide for Federal agency personnel with additional information incorporated by references. The steps to be followed in selecting, procuring, and installing a photovoltaic application are given.

  10. Control design and robustness analysis of a ball and plate system by using polynomial chaos

    SciTech Connect

    Colón, Diego; Balthazar, José M.; Reis, Célia A. dos; Bueno, Átila M.; Diniz, Ivando S.; Rosa, Suelia de S. R. F.

    2014-12-10

    In this paper, we present a mathematical model of a ball and plate system, a control law and analyze its robustness properties by using the polynomial chaos method. The ball rolls without slipping. There is an auxiliary robot vision system that determines the bodies' positions and velocities, and is used for control purposes. The actuators are to orthogonal DC motors, that changes the plate's angles with the ground. The model is a extension of the ball and beam system and is highly nonlinear. The system is decoupled in two independent equations for coordinates x and y. Finally, the resulting nonlinear closed loop systems are analyzed by the polynomial chaos methodology, which considers that some system parameters are random variables, and generates statistical data that can be used in the robustness analysis.

  11. Control design and robustness analysis of a ball and plate system by using polynomial chaos

    NASA Astrophysics Data System (ADS)

    Colón, Diego; Balthazar, José M.; dos Reis, Célia A.; Bueno, Átila M.; Diniz, Ivando S.; de S. R. F. Rosa, Suelia

    2014-12-01

    In this paper, we present a mathematical model of a ball and plate system, a control law and analyze its robustness properties by using the polynomial chaos method. The ball rolls without slipping. There is an auxiliary robot vision system that determines the bodies' positions and velocities, and is used for control purposes. The actuators are to orthogonal DC motors, that changes the plate's angles with the ground. The model is a extension of the ball and beam system and is highly nonlinear. The system is decoupled in two independent equations for coordinates x and y. Finally, the resulting nonlinear closed loop systems are analyzed by the polynomial chaos methodology, which considers that some system parameters are random variables, and generates statistical data that can be used in the robustness analysis.

  12. Present-day plate motions: Retrieval from the TOPEX/Poseidon orbitography network (DORIS system)

    NASA Technical Reports Server (NTRS)

    Souriau, Annie; Cazenave, Anny; Biancale, R.; Balmino, G.; Dominh, K.; Mazzega, P.; Lemoine, J.-M.; Boucher, Claude; Willis, P.; Kasser, M.

    1991-01-01

    The goal of the proposal is to determine the present motion of the main tectonic plates from the Doppler data of the Doppler Orbitography and Radiopositioning Integrated by Satellite (DORIS) orbitography system, which includes in its final configuration about 50 tracking stations with a world-wide distribution.

  13. Plate coil thermal test bench for the Daniel K. Inouye Solar Telescope (DKIST) carousel cooling system

    NASA Astrophysics Data System (ADS)

    Phelps, LeEllen; Murga, Gaizka; Montijo, Guillermo; Hauth, David

    2014-08-01

    Analyses have shown that even a white-painted enclosure requires active exterior skin-cooling systems to mitigate dome seeing which is driven by thermal nonuniformities that change the refractive index of the air. For the Daniel K. Inouye Solar Telescope (DKIST) Enclosure, this active surface temperature control will take the form of a system of water cooled plate coils integrated into the enclosure cladding system. The main objective of this system is to maintain the surface temperature of the enclosure as close as possible to, but always below, local ambient temperature in order to mitigate this effect. The results of analyses using a multi-layer cladding temperature model were applied to predict the behavior of the plate coil cladding system and ultimately, with safety margins incorporated into the resulting design thermal loads, the detailed designs. Construction drawings and specifications have been produced. Based on these designs and prior to procurement of the system components, a test system was constructed in order to measure actual system behavior. The data collected during seasonal test runs at the DKIST construction site on Haleakalā are used to validate and/or refine the design models and construction documents as appropriate. The test fixture was also used to compare competing hardware, software, components, control strategies, and configurations. This paper outlines the design, construction, test protocols, and results obtained of the plate coil thermal test bench for the DKIST carousel cooling system.

  14. Design and test of a pumped two-phase mounting plate. [for spacecraft thermal control systems

    NASA Technical Reports Server (NTRS)

    Grote, M. G.; Swanson, T. D.

    1985-01-01

    The design, fabrication, and testing of the full-scale development unit of a pumped two-phase mounting plate (TPMP) used in advanced two-phase spacecraft thermal control systems are described. The mounting plate is tested with R-11 in the evaporator mode for total heat loads of over 3000 watts and local heat fluxes over 4 W/sq cm, and in the condenser mode with condenser loads from 60 to 400 watts and inlet qualities from 8 to 94 percent. The calculated heat-transfer coefficients are between 0.66 and 1.0 W/sq cm/C and are nearly independent of the flow rate and heat load except at very low heat loads. It is shown that the TPMP can be run with inlet conditions down to 22 C subcooling without any significant gradients in the plate and that it performs well with nonuniform heat fluxes.

  15. Operational experience with intermediate flat-plate photovoltaic systems

    NASA Astrophysics Data System (ADS)

    Risser, V. V.; Zwibel, H. S.

    Operating features, data acquisition, and fault isolation and maintenance procedures at 20 kWp and 100 kWp photovoltaic (PV) installations in Texas and New Mexico are discussed. Weather and system performance are sensed each minute, averages are calculated for each ten readings, and data is stored on magnetic tape. A total of 84 parameters, including 64 string currents, are recorded at the 20 kWp array and 84 parameters, with 42 string currents, are traced in New Mexico. The 20 kW array is coupled to a 197 MW utility power plant, which determines the voltage of the array. It produced 12 MWh in one yr of operation, functioning at 24 pct overall efficiency. The 100 kWp system is coupled to a 60 kW power conditioning unit and feeds a shopping center, producing 8 pct of the annual load with a cap factor of 25 pct and 192 MWh of dc current produced in one year. It was found that under normal conditions washing the panels is not economically justified in terms of the small power lost if washing does not occur. It is concluded that the PV arrays can be successfully used in an automated operation mode.

  16. Measurements of Conversion Efficiency for a Flat Plate Thermophotovoltaic System Using a Photonic Cavity Test System

    SciTech Connect

    E.J. Brown; C.T. Ballinger; S.R. Burger; G.W. Charache; L.R. Danielson; D.M. DePoy; T.J. Donovan; M. LoCascio

    2000-05-30

    The performance of a 1 cm{sup 2} thermophotovoltaic (TPV) module was recently measured in a photonic cavity test system. A conversion efficiency of 11.7% was measured at a radiator temperature of 1076 C and a module temperature of 29.9 C. This experiment achieved the highest direct measurement of efficiency for an integrated TPV system. Efficiency was calculated from the ratio of the peak (load matched) electrical power output and the heat absorption rate. Measurements of these two parameters were made simultaneously to assure the validity of the measured efficiency value. This test was conducted in a photonic cavity which mimicked a typical flat-plate TPV system. The radiator was a large, flat graphite surface. The module was affixed to the top of a copper pedestal for heat absorption measurements. The heat absorption rate was proportional to the axial temperature gradient in the pedestal under steady-state conditions. The test was run in a vacuum to eliminate conductive and convective heat transfer mechanisms. The photonic cavity provides the optimal test environment for TPV efficiency measurements because it incorporates all important physical phenomena found in an integrated TPV system: high radiator emissivity and blackbody spectral shape, photon recycling, Lambertian distribution of incident radiation and complex geometric effects. Furthermore, the large aspect ratio between radiating surface area and radiator/module spacing produces a view factor approaching unity with minimal photon leakage.

  17. Proceedings of the flat-plate solar array project research forum on photovoltaic metallization systems

    SciTech Connect

    1983-11-15

    A Photovoltaic Metallization Research Forum, under the sponsorship of the Jet Propulsion Laboratory's Flat-Plate Solar Array Project and the US Department of Energy, was held March 16-18, 1983 at Pine Mountain, Georgia. The Forum consisted of five sessions, covering (1) the current status of metallization systems, (2) system design, (3) thick-film metallization, (4) advanced techniques and (5) future metallization challenges. Twenty-three papers were presented.

  18. Analytical model development of an eddy-current-based non-contacting steel plate conveyance system

    NASA Astrophysics Data System (ADS)

    Liu, Cheng-Tsung; Lin, Sheng-Yang; Yang, Yung-Yi; Hwang, Chang-Chou

    A concise model for analyzing and predicting the quasi-static electromagnetic characteristics of an eddy-current-based non-contacting steel plate conveyance system has been developed. Confirmed by three-dimensional (3-D) finite element analysis (FEA), adequacy of the analytical model can be demonstrated. Such an effective approach, which can be conveniently used by the potential industries for preliminary system operational performance evaluations, will be essential for designers and on-site engineers.

  19. A 6 x 6 drop plate method for simultaneous colony counting and MPN enumeration of Campylobacter jejuni, Listeria monocytogenes, and Escherichia coli.

    PubMed

    Chen, Chin Yi; Nace, Gary W; Irwin, Peter L

    2003-11-01

    A protocol was developed using 96-well plates and multichannel pipettes for serial dilutions, followed by drop plating on agar in a 6 x 6 format. This protocol permits simultaneous plating of six dilutions which greatly decreases the number of plates utilized thereby saving incubator space for organisms such as Campylobacter which require unique environmental conditions.

  20. Liquid chromatography/tandem mass spectrometric bioanalysis using normal-phase columns with aqueous/organic mobile phases - a novel approach of eliminating evaporation and reconstitution steps in 96-well SPE.

    PubMed

    Naidong, Weng; Shou, Wilson Z; Addison, Thomas; Maleki, Saber; Jiang, Xiangyu

    2002-01-01

    Bioanalytical methods using automated 96-well solid-phase extraction (SPE) and liquid chromatography with electrospray tandem mass spectrometry (LC/MS/MS) are widely used in the pharmaceutical industry. SPE methods typically require manual steps of drying of the eluates and reconstituting of the analytes with a suitable injection solvent possessing elution strength weaker than the mobile phase. In this study, we demonstrated a novel approach of eliminating these two steps in 96-well SPE by using normal-phase LC/MS/MS methods with low aqueous/high organic mobile phases, which consisted of 70-95% organic solvent, 5-30% water, and small amount of volatile acid or buffer. While the commonly used SPE elution solvents (i.e. acetonitrile and methanol) have stronger elution strength than a mobile phase on reversed-phase chromatography, they are weaker elution solvents than a mobile phase for normal-phase LC/MS/MS and therefore can be injected directly. Analytical methods for a range of polar pharmaceutical compounds, namely, omeprazole, metoprolol, fexofenadine, pseudoephedrine as well as rifampin and its metabolite 25-desacetyl-rifampin, in biological fluids, were developed and optimized based on the foregoing principles. As a result of the time saving, a batch of 96 samples could be processed in one hour. These bioanalytical LC/MS/MS methods were validated according to "Guidance for Industry - Bioanalytical Method Validation" recommended by the Food and Drug Administration (FDA) of the United States.

  1. β-Cyclodextrin enhanced on-line organic solvent field-amplified sample stacking in capillary zone electrophoresis for analysis of ambroxol in human plasma, following liquid-liquid extraction in the 96-well format.

    PubMed

    Li, Ji; Bi, Youwei; Wang, Li; Sun, Fanlu; Chen, Zhao; Xu, Guili; Fan, Guorong

    2012-07-01

    A field-amplified sample stacking (FASS) and capillary zone electrophoresis (CZE) method is described for the quantification of ambroxol hydrochloride in human plasma, following liquid-liquid extraction in the 96-well format. The separation was carried out at 25 °C in a 31.2 cm × 75 μm fused-silica capillary with an applied voltage of 15 kV. The background electrolyte (BGE) was composed of 6.25 mM borate-25 mM phosphate (pH 3.0) and 1mM β-cyclodextrin. The detection wavelength was 210 nm. Clean-up and preconcentration of plasma biosamples were developed by 96-well format liquid-liquid extraction (LLE). In this study, FASS in combination with β-cyclodextrin enhanced the sensitivity about 60-70 fold in total. The method was suitably validated with respect to stability, specificity, linearity, lower limit of quantitation, accuracy, precision, extraction recovery and robustness. The calibration graph was linear for ambroxol hydrochloride from 2 to 500 ng/ml. The lower limit of quantification was 2 ng/ml. The intra- and inter-day precisions of lowest limit of quantification (LLOQ) were 9.61 and 11.80%, respectively. The method developed was successfully applied to the evaluation of clinical pharmacokinetic study of ambroxol hydrochloride tablet after oral administration to 12 healthy volunteers.

  2. Managing Vancouver B1 fractures by cerclage system compared to locking plate fixation - a biomechanical study.

    PubMed

    Gordon, Katharina; Winkler, Martin; Hofstädter, Thomas; Dorn, Ulrich; Augat, Peter

    2016-06-01

    With increasing life expectancy and number of total hip arthroplasties (THA), the need for revision surgery is increasing too. The aim of this study was to evaluate the optimal fracture treatment for a clinically characteristic Vancouver B1 fracture. We hypothesized that locking plate fixation has biomechanical advantages over fixation with a simple cerclage system. Additionally, we hypothesized that removal of the primary short stem and revision with a long stem would show biomechanical benefit. The biomechanical testing was performed with a static and a dynamic loading protocol on twenty 4th Generation sawbones. These were divided into four different groups (n = 5 each). In group 1, the primary uncemented short stem remained and the fracture was stabilized with a locking plate. In group 2, the primary stem remained and the fracture was stabilized with a cerclage stabilization system containing two stabilizers and four cerclages. In group 3, the primary stem was replaced by an uncemented long revision stem and the fracture was fixed with a locking plate. In group 4, the short stem was replaced by a long revision stem and the fracture was fixed with the cerclage system. Static testing revealed that the revision of the short stem with the long stem caused a 2-fold (p < 0.001, ANOVA) increase of axial stiffness. In dynamic testing, the number of cycles to failure was 4 times (p < 0.001, ANOVA) higher with the long revision stem. Compared to locked plating cerclage wiring demonstrated a 26% more cycles to failure (p = 0.031, ANOVA). The load to failure was 91% larger (p < 0.001, ANOVA) with the long revision stem and 11% smaller with locked plating (p < 0.001, ANOVA). In conclusion, the present biomechanical study indicates that periprosthetic Vancouver B1 fractures can be sufficiently fixed by simple cerclage systems. Revision with a long replacement stem provides a superior mechanical stability regardless of type of osteosynthesis fixation and is therefore a viable

  3. Development of ink transfer monitoring system for roll-to-plate gravure offset printing process

    NASA Astrophysics Data System (ADS)

    Lee, Seung-Hyun; Lee, Taik-Min; Kim, Dong-Soo; Kim, Byoung Jae; Lee, Seungwoo

    2010-11-01

    The gravure offset printing process is very cost-effective for printed electronics, such as printed solar cell, printed battery, printed TFT, printed RFID tag and so on. In gravure offset printing, there are two kinds of ink transfer processes -- off and set processes. At the off process, an elastic blanket cylinder picks up the ink from patterned plate or patterned cylinder. At the set process, ink on the elastic blanket cylinder is transferred onto the target substrate. These two ink transfer processes determine printing quality, therefore understanding of ink transfer mechanism during off and set processes are very important to control printing quality. In this study, we developed ink transfer monitoring system for roll-to-plate gravure printing. We visualized ink transfer from pattern plate to rolling blanket cylinder (off process) and from rolling blanket cylinder to plate substrate (set process) by using high-speed camera and long range microscope. We investigated the effects of pattern size, printing speed, rotational effect of blanket cylinder, contact angle and rheological property of ink to understanding gravure offset printing mechanism.

  4. Development of Portable Automatic Number Plate Recognition System on Android Mobile Phone

    NASA Astrophysics Data System (ADS)

    Mutholib, Abdul; Gunawan, Teddy S.; Chebil, Jalel; Kartiwi, Mira

    2013-12-01

    The Automatic Number Plate Recognition (ANPR) System has performed as the main role in various access control and security, such as: tracking of stolen vehicles, traffic violations (speed trap) and parking management system. In this paper, the portable ANPR implemented on android mobile phone is presented. The main challenges in mobile application are including higher coding efficiency, reduced computational complexity, and improved flexibility. Significance efforts are being explored to find suitable and adaptive algorithm for implementation of ANPR on mobile phone. ANPR system for mobile phone need to be optimize due to its limited CPU and memory resources, its ability for geo-tagging image captured using GPS coordinates and its ability to access online database to store the vehicle's information. In this paper, the design of portable ANPR on android mobile phone will be described as follows. First, the graphical user interface (GUI) for capturing image using built-in camera was developed to acquire vehicle plate number in Malaysia. Second, the preprocessing of raw image was done using contrast enhancement. Next, character segmentation using fixed pitch and an optical character recognition (OCR) using neural network were utilized to extract texts and numbers. Both character segmentation and OCR were using Tesseract library from Google Inc. The proposed portable ANPR algorithm was implemented and simulated using Android SDK on a computer. Based on the experimental results, the proposed system can effectively recognize the license plate number at 90.86%. The required processing time to recognize a license plate is only 2 seconds on average. The result is consider good in comparison with the results obtained from previous system that was processed in a desktop PC with the range of result from 91.59% to 98% recognition rate and 0.284 second to 1.5 seconds recognition time.

  5. Performance Assessment of Two Different Aviation CARC Coating Systems on Steel When Cadmium Plating is Eliminated (Briefing Charts)

    DTIC Science & Technology

    2006-01-25

    System Description 1 Cd Plating with MIL-PRF-23377C 1 1 15 1 1 1D Cd Plating with MIL-PRF-23377C Damaged 1 4 6 1 1 2 Unplated with MIL-PRF-23377C 80 80 80 80 ... 80 2D Unplated with MIL-PRF-23377C Damaged 7 9 48 9 8 3 Cd Plating with MIL-PRF-23377N 4 15 26 8 4 3D Cd Plating with MIL-PRF-23377N Damaged 1

  6. McClellan Air Force Base plating shop rinse water recycle system

    SciTech Connect

    Miyasaki, D.H.; LaRue, D.M.

    1988-01-01

    The Idaho National Engineering Laboratory performed an assessment on the technical and economic viability of a total recycle of the rinse water generated by the Sacramento Air Logistics Center Plating Shop, located in Building 243-G of McClellan Air Force Base. A proposed conceptual design using ion exchange beds for the recycle of plating shop rinse water is presented and evaluated. Laboratory studies indicated that the proposed recycle system meets all functional and operational requirements that were identified. But concern over the amount of waste generated during the process of regenerating the ion exchange columns prompted an evaluation of a reverse osmosis unit, which would function as pretreatment before the ion exchange process. Further studies indicated that the addition of this pretreatment process would improve the performance of the recycle system, but that the cost of construction and operation would be significantly higher. Based on technical and economic evaluations, the ion exchange system is the recommended recycle system for the plating shop. 9 figs.

  7. Formation of Island Arc-Trench System due to Plate Subduction on the Basis of Elastic Dislocation Theory

    NASA Astrophysics Data System (ADS)

    Fukahata, Y.; Matsu'ura, M.

    2015-12-01

    The most conspicuous cumulative deformation in subduction zones is the formation of island arc-trench system. A pair of anomalies in topography and free-air gravity, high in the arc and low around the trench, is observed without exceptions all over the world. Since the 1960s, elastic dislocation theory has been widely used to interpret coseismic crustal deformation. For the modeling of longer-term crustal deformation, it is necessary to consider viscoelastic properties of the asthenosphere. By simply applying elastic-viscoelastic dislocation theory to plate subduction, Matsu'ura and Sato (1989, GJI) have shown that some crustal deformation remains after the completion of one earthquake cycle, which means that crustal deformation accumulates with time in a long term due to plate subduction. In fact, by constructing a plate interface model in and around Japan, Hashimoto, Fukui and Matsu'ura (2004, PAGEOPH) have demonstrated that the computed vertical displacements due to steady plate subduction well explain the observed free-air gravity anomaly pattern. Recently, we got a lucid explanation of crustal deformation due to plate subduction. In subduction zones, oceanic plates bend and descend into the mantle. Because the bending of oceanic plates is usually not spontaneous, there exists kinematic interaction between the oceanic and overriding plates, which causes cumulative deformation of the overriding plate. This may be understood based on the law of action and reaction: one is bending of an oceanic plate and the other is deformation of the overriding plate. As a special case, it is useful to consider plate subduction along a part of true circle. In this case, crustal deformation due to steady subduction is solely caused by the effect of gravity, because dislocation along a circle does not cause any intrinsic internal deformation. When an oceanic plate is descending along an arcuate plate interface from the right-hand side, according to dislocation theory, the oceanic

  8. Thickness measurement system for transparent plates using dual digital versatile disc (DVD) pickups.

    PubMed

    Liu, Chien-Hung; Yeh, Shien-Chang; Huang, Hsueh-Liang

    2010-02-01

    A low-cost high-precision thickness measurement system for transparent plates that uses dual digital versatile disc (DVD) pickups is proposed. The two DVD pickups are used as the transmitter and the receiver in the measurement system, respectively. One of the DVD pickups emits a laser to the other DVD pickup (receiver) and projects on the photodiode integrated circuit of the receiver. The transparent plate is placed in the optical path to change the focused point that will affect the focusing error signal (FES) of the receiver. Using the FES, a mathematical model for thickness measurement based on the geometric optical method is developed. The experimental results show that the accuracy is 1.5 microm, and the uncertainty is estimated to be +/-1.37 microm for the measured thickness of 150 microm.

  9. Thickness measurement system for transparent plates using dual digital versatile disc (DVD) pickups

    SciTech Connect

    Liu, Chien-Hung; Yeh, Shien-Chang; Huang, Hsueh-Liang

    2010-02-01

    A low-cost high-precision thickness measurement system for transparent plates that uses dual digital versatile disc (DVD) pickups is proposed. The two DVD pickups are used as the transmitter and the receiver in the measurement system, respectively. One of the DVD pickups emits a laser to the other DVD pickup (receiver) and projects on the photodiode integrated circuit of the receiver. The transparent plate is placed in the optical path to change the focused point that will affect the focusing error signal (FES) of the receiver. Using the FES, a mathematical model for thickness measurement based on the geometric optical method is developed. The experimental results show that the accuracy is 1.5 {mu}m, and the uncertainty is estimated to be {+-}1.37 {mu}m for the measured thickness of 150{mu}m.

  10. Clinical application of locked plating system in children. An orthopaedic view

    PubMed Central

    Zafra-Jimenez, Jose Alberto; Rodriguez Martin, Juan

    2010-01-01

    In recent years, the locked plating system has gained favour in the treatment of certain fractures in adults; however, there is not much information regarding its use in children. We think there could be some advantages and applications such as: an alternative to external fixation, the bridge plating technique, unicortical screws, removal of hardware, metadiaphyseal fractures, periarticular fractures, poor quality bone, and allograft fixation. However, there are some disadvantages to keep in mind and the final decision for using it should be based on the osteosynthesis method principle the surgeon would like to apply. In this review article we discuss the up-to-date possible clinical applications and issues of this system. PMID:20162415

  11. A novel disturbance-observer based friction compensation scheme for ball and plate system.

    PubMed

    Wang, Yongkun; Sun, Mingwei; Wang, Zenghui; Liu, Zhongxin; Chen, Zengqiang

    2014-03-01

    Friction is often ignored when designing a controller for the ball and plate system, which can lead to steady-error and stick-slip phenomena, especially for the small amplitude command. It is difficult to achieve high-precision control performance for the ball and plate system because of its friction. A novel reference compensation strategy is presented to attenuate the aftereffects caused by the friction. To realize this strategy, a linear control law is proposed based on a reduced-order observer. Neither the accurate friction model nor the estimation of specific characteristic parameters is needed in this design. Moreover, the describing function method illustrates that the limit cycle can be avoided. Finally, the comparative mathematical simulations and the practical experiments are used to validate the effectiveness of the proposed method.

  12. Hydrogen sensing in vacuum systems with catalytic field plate MNOS capacitors

    NASA Astrophysics Data System (ADS)

    Hughes, R. C.; Bastasz, R.; Ellis, W. P.

    1997-05-01

    The rate of response of catalytic field plate MNOS capacitors to steps in hydrogen partial pressure in a ultra high vacuum (UHV) system is reported. The steps in pressure are provided by a gas pulse valve that allows sub-second increases in the pressure. A combination of light sputter cleaning and elevated sensor temperature produces a sensor response fast enough to be of interest in the monitoring of hydrogen partial pressures in a processing chamber.

  13. Radiation-hard ceramic Resistive Plate Chambers for forward TOF and T0 systems

    NASA Astrophysics Data System (ADS)

    Akindinov, A.; Dreyer, J.; Fan, X.; Kämpfer, B.; Kiselev, S.; Kotte, R.; Garcia, A. Laso; Malkevich, D.; Naumann, L.; Nedosekin, A.; Plotnikov, V.; Stach, D.; Sultanov, R.; Voloshin, K.

    2017-02-01

    Resistive Plate Chambers with ceramic electrodes are the main candidates for a use in precise multi-channel timing systems operating in high-radiation conditions. We report the latest R&D results on these detectors aimed to meet the requirements of the forward T0 counter at the CBM experiment. RPC design, gas mixture, limits on the bulk resistivity of ceramic electrodes, efficiency, time resolution, counting rate capabilities and ageing test results are presented.

  14. Integration of image analysis and robotics into a fully automated colony picking and plate handling system.

    PubMed Central

    Jones, P; Watson, A; Davies, M; Stubbings, S

    1992-01-01

    We describe here the integration of image analysis and robotics to produce a fully automated colony picking/plate handling system. Biological tests were performed to verify its performance in terms of sterilisation and accuracy of picking. The machine was then used by a single operative to pick a 36,000 clone cDNA library in approximately 42 hrs over 5 days. Images PMID:1408762

  15. A planar EMT system for the detection of faults on thin metallic plates

    NASA Astrophysics Data System (ADS)

    Yin, W.; Peyton, A. J.

    2006-08-01

    This paper describes a novel planar electromagnetic tomography system for the detection of conductivity inhomogeneity on a metallic plate. The proposed system differs from traditional electromagnetic inductance tomography (EMT) systems in its spatial arrangements of coils. Sensor coils are distributed to form a circular array with their axes not parallel but perpendicular to the plate under inspection. The forward solution for the sensor array next to a homogeneous conductive plate is based on the analytical solution provided by Cheng. The sensitivity matrix for a prototype sensor was computed by numerical evaluation of the analytical solution. For the inverse solution, a modified Newton-Raphson method was used to adjust the conductivity distribution to fit a set of inductances measured from the sensor array in a least-squared sense. Frequency- dependent sensitivity analysis was performed to find an optimum testing frequency. The far-field and near-field effects in electrical tomography are discussed. Good estimates for the conductivity distribution were obtained at the optimum frequency. Experimental tests were performed by taking the difference in mutual inductance of the coil pairs when placed next to a homogeneous reference conductor and next to a conductor with faults. Inverse results based on experimental data verified this method.

  16. Lamb shift of non-degenerate energy level systems placed between two infinite parallel conducting plates

    NASA Astrophysics Data System (ADS)

    Billaud, B.; Truong, T.-T.

    2013-01-01

    In this paper, the Lamb shift in systems with non-degenerate energy levels, put in the electromagnetic environment provided by two infinite parallel conducting plates, is analyzed. An explicit formula giving the relative Lamb shift (as compared to the standard one in a vacuum) is derived for spherical semiconductor quantum dots (QDs), via a careful mathematical treatment of divergences in the calculations using the theory of distributions. This result settles a controversy between two different formulas existing in the current literature. Its sensitive dependence on the plates separation may be viewed as an indirect manifestation of the Lamb shift and may be used for the fine tuning of the QD non-degenerate energy spectrum in some experimental contexts.

  17. A hybrid wave propagation and statistical energy analysis on the mid-frequency vibration of built-up plate systems

    NASA Astrophysics Data System (ADS)

    Ma, Yongbin; Zhang, Yahui; Kennedy, David

    2015-09-01

    Based on the concept of the hybrid finite element (FE) analysis and statistical energy analysis (SEA), a new hybrid method is developed for the mid-frequency vibration of a system comprising rectangular thin plates. The wave propagation method based on symplectic analysis is used to describe the vibration of the deterministic plate component. By enforcing the displacement continuity and equilibrium of force at the connection interface, the dynamic coupling between the deterministic plate component and the statistical plate component described by SEA is established. Furthermore, the hybrid solution formulation for the mid-frequency vibration of the system built up by plates is proposed. The symplectic analytical wave describing the deterministic plate component eliminates the boundary condition limitation of the traditional analytical wave propagation method and overcomes the numerical instability of numerical wave propagation methods. Numerical examples compare results from the proposed method with those from the hybrid FE-SEA method and the Monte Carlo method. The comparison illustrates that the proposed method gives good predictions for the mid-frequency behavior of the system considered here with low computational time. In addition, a constant proportionality coefficient between the system coupling power and the energy difference between the plate components can be found, when external forces are applied at different locations on a line perpendicular to the wave propagation direction. Based on this finding, two fast solution techniques are developed for the energy response of the system, and are validated by numerical examples.

  18. Advanced Extended Plate and Beam Wall System in a Cold-Climate House

    SciTech Connect

    Mallay, Dave; Wiehagen, Joseph; Kochkin, Vladimir

    2016-01-01

    This report presents the design and evaluation of an innovative wall system. This highly insulated (high-R) light-frame wall system for use above grade in residential buildings is referred to as Extended Plate & Beam (EP&B). The EP&B design is the first of its kind to be featured in a new construction test house (NCTH) for the DOE Building America program. The EP&B wall design integrates standard building methods and common building products to construct a high-R wall that minimizes transition risks and costs to builders.

  19. Clinical evaluation of a flat-plate membrane plasma exchange system.

    PubMed

    Grossman, L; Benny, W B; Buchanan, J; Erickson, R R; Buffaloe, G W

    1983-01-01

    A new flat-plate membrane plasma separation system specifically designed for therapeutic plasma exchange (TPE) was clinically evaluated in both research and routine clinical settings. The study included a comparison to a currently available centrifugal cell separation system employed for TPE. A total of 267 membrane procedures were performed on 39 patients over a 14-month period. Both qualitative and quantitative studies showed that membrane plasma exchange procedures were equivalent to centrifugal procedures in the removal of plasma constituents from patients. A notable difference between the two types of procedure was the effect on the peripheral blood platelet count: the plasma filtrate from the membrane system was essentially cell-free and platelet counts fell only 11% during the procedure, compared to a 53% decrease during the centrifugation runs. Patient responses to both types of procedure were similar and the frequency of side-effects was low. A sampling of patient opinion revealed a preference for the membrane system for a variety of reasons. Procedure times were shorter with the membrane system because of higher achievable blood flow rates, and thus higher plasma exchange rates, while the overall nursing time requirement was lower. The results show that this flat-plate membrane TPE system enables rapid and effective plasma exchange therapy, and offered a number of monitoring and control functions that provided a safer, more efficient therapeutic procedure in the majority of patient treatments performed in this study.

  20. Microtiter plate-based antibody microarrays for bacteria and toxins

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Research has focused on the development of rapid biosensor-based, high-throughput, and multiplexed detection of pathogenic bacteria in foods. Specifically, antibody microarrays in 96-well microtiter plates have been generated for the purpose of selective detection of Shiga toxin-producing E. coli (...

  1. PERPENDICULAR DOUBLE-PLATE FIXATION WITH LOCKING SYSTEM FOR ACROMION PEDICLE FRACTURE

    PubMed Central

    Zhu, Junkun; Pan, Zhijun; Zheng, Rongzong; Lan, Shuhua

    2016-01-01

    Objective : To describe the surgical technique and preliminary clinical outcomes in a series of open reduction internal fixation of basal acromion process fractures applying a double-plating technique. Methods : Nine consecutive patients, mean age 33.4 years old (range, 23-61 years old) with unilateral acromion fracture (Type 3 AO/OTA) with more than 1cm displacement who underwent fixation utilizing a locked double-plating technique, were evaluated on average at 7.8 months (range, 3-15 months) for outcomes related to pain, shoulder function, and surgical complications. Results : Eight patients recovered with complete radiographic union and favorable shoulder function. One case failed to be fully evaluated for more than 3 months follow-up. The overall scores of Constant, Shoulder Pain and Disability Index (SPADI) and DASH for the eight patients reviewed were 91.9± 6.31, 3.11± 3.79 and 5.2± 6.35, respectively. No post-operative infection or surgical hardware irritation was identified at final follow-up of these eight patients. Conclusion : While more evidence is needed to justify its advantages over traditional implants, perpendicular double-plate with a locking system may be indicated for acromion pedicle fracture treatment, since it performed well for fracture healing and joint function rehabilitation. Level of Evidence IV, Therapeutic Study. PMID:26981047

  2. A multichannel deflection plates control system for the ALF facility at the APS.

    SciTech Connect

    Deriy, B.; Accelerator Systems Division

    2006-01-01

    A deflection plates control system was developed as part of SPIRIT (Single Photon Ionization/Resonant Ionization to Threshold), a new secondary neutral mass spectrometry (SNMS) instrument that uses tunable vacuum ultraviolet light from the APS ALF (Argonne Linear Free-electron laser) facility for postionization. The system comprises a crate controller with PC104 embedded computer, 32 amplifiers, and two 1-kV power supplies. Thirty-two D/A converters are used to control voltages at the deflection plates within {+-} 400 V with 100-mV resolution. An algorithm for simultaneous sweeping of up to 16 XY areas with 10-{micro}s time resolution also has been implemented in the embedded computer. The purpose of the system is to supply potentials to various ion optical elements for electrostatic control of keV primary and secondary ion beams in this SNMS instrument. The control system is of particular value in supplying (1) bipolar potentials for steering ions, (2) multiple potentials for octupole lenses that shape the ion beams, and (3) ramped deflection potentials for rastering the primary ion beam. The system has been in use as part of the SPIRIT instrument at the ALF facility since 2002.

  3. Advanced Extended Plate and Beam Wall System in a Cold-Climate House

    SciTech Connect

    Mallay, Dave; Wiehagen, Joseph; Kochkin, Vladimir

    2016-01-29

    This report presents the design and evaluation of an innovative wall system. This highly insulated (high-R) light-frame wall system for use above grade in residential buildings is referred to as Extended Plate & Beam (EP&B). The EP&B design is the first of its kind to be featured in a new construction test house (NCTH) for the DOE Building America program. The EP&B wall design integrates standard building methods and common building products to construct a high-R wall that minimizes transition risks and costs to builders. The EP&B design combines optimized framing with integrated rigid foam sheathing to increase the wall system's R-value and reduce thermal bridging. The foam sheathing is installed between the wall studs and structural wood sheathing. The exterior wood sheathing is attached directly to a framing extension formed by extended top and bottom plates. The exterior wood sheathing can dry to the exterior and provides bracing, a clear drainage plane and flashing surface for window and door openings, and a nailing surface for siding attachment. With support of the DOE Building America program, Home Innovation Research Labs partnered with Lancaster County Career and Technology Center (LCCTC) to build a NCTH in Lancaster, PA to demonstrate the EP&B wall design in a cold climate (IECC climate zone 5A). The results of the study confirmed the benefits of the systems and the viability of its integration into the house construction process.

  4. Analysis on strength and stiffness of double-deck plates filter system of mechanical water treatment plant

    NASA Astrophysics Data System (ADS)

    Feng, De-zhen; Yu, Qi-qi

    2017-01-01

    Domestic water treatment is a very important technology field. Now, mechanical water treatment technology is getting wide use in production. In the process of life water treatment, filter process is a very important step. In this paper, the strength and deformation of double-deck plates filter system which includes upper filter plate, lower filter plate and reinforced ribs were analyzed with ANSYS and useful results were got. Through the analysis on strength and deformation, the paper found the advantages and disadvantages of production and design of filter systems. After analyzing and comparing the stresses and deformations of several different design schemes, the paper provided the optimized design plan of filter system which can satisfy the strength need and decrease the creep deformation of plastic filter plates.

  5. Korean Type Distal Radius Anatomical Volar Plate System: A Preliminary Report

    PubMed Central

    Kim, Jeong Hwan; Kim, Jihyeung; Kim, Min Bom; Rhee, Seung Hwan; Gong, Hyun Sik; Lee, Young Ho

    2014-01-01

    Background Distal radius fracture is the most common fracture of the upper extremity, and approximately 60,000 distal radius fractures occur annually in Korea. Internal fixation with an anatomical volar locking plate is widely used in the treatment of unstable distal radius fractures. However, most of the currently used distal radius anatomical plate systems were designed based on the anatomical characteristics of Western populations. Recently, the Korean-type distal radius anatomical volar plate (K-DRAVP) system was designed and developed based on the anatomical characteristics of the distal radius of Koreans. The purpose of this study was to evaluate the preliminary results of the new K-DRAVP system, and to compare its radiologic and functional results with those of the other systems. Methods From March 2012 to October 2012, 46 patients with acute distal radius fractures who were treated with the K-DRAVP system at three hospitals were enrolled in this study. Standard posteroanterior and lateral radiographs were obtained to assess fracture healing, and three radiographic parameters (volar tilt, radial inclination, and radial length) were assessed to evaluate radiographic outcomes. The range of motion and grip strength, the Gartland and Werley scoring system, and the disabilities of the arm, shoulder and hand (DASH) questionnaire were used to assess clinical and functional outcomes. Results All radiologic parameters were restored to normal values, and maintained without any loosening or collapse until the time of final follow-up. Grip strength was restored to 84% of the value for the unaffected side. The mean range of motion of the wrist at final follow-up was restored to 77%-95% of the value for the unaffected side. According to the Gartland and Werley scoring system, there were 16 excellent, 26 good, and 4 fair results. The mean DASH score was 8.4 points. There were no complications after surgery. Conclusions The newly developed K-DRAVP system could be used to

  6. Biomechanical design of less invasive stabilization system femoral plates: computational evaluation of the fracture environment.

    PubMed

    Reina-Romo, Esther; Giráldez-Sánchez, Ma; Mora-Macías, J; Cano-Luis, P; Domínguez, Jaime

    2014-10-01

    Less Invasive Stabilization System femoral plates are currently accepted as a suitable fixation technique for supra-intercondylar femoral fractures. However, general agreement does not exist regarding the optimum design of this fixator type. Therefore, the aim of this article is to reduce the intrinsic Less Invasive Stabilization System complications by clarifying, from a biomechanical point of view, how the number of screws, the screw connection type (unicortical or bicortical), or the structured position of the screws can influence the outcome of the fracture site. These studies include a specific finite element analysis that determines how several biomechanical variables, such as the movement at the fracture site, are influenced by the preconditions of bone healing. The results of this study show that the screw type affects the mechanical stabilization of the femur to a greater extent than the material type of the Less Invasive Stabilization System femoral plates. The most significant differences among all the analyzed configurations are observed in the shear interfragmentary strain between screw types. Values are approximately 50% higher with unicortical screws than with bicortical ones.

  7. Development of a nickel plated aluminum krypton-81m target system.

    PubMed

    Alrumayan, F; Okarvi, S M; Nagatsu, K; Yanbawi, S; Aljammaz, I

    2017-03-01

    A fully automated system was developed to produce rubidium-81 ((81)Rb), based on the (nat)Kr (p, n) (81)Rb reaction. The energy incident on the target was 26MeV. Only 6MeV was stopped inside the gas and the remainder was stopped by a specially designed flange. The target body was characterized by its conical shape and its inner walls were chemically plated with 100±10µm of nickel (Ni). Ni is advantageous as a fairly good conductor of heat whose surface can resist solutions. Additionally, the Ni plated target allowed potassium chloride to dissolve (81)Rb, with no further effect on the target body. The system produced (81)Rb with a production yield of approximately 4.5mCi/µAh, which is close to the calculated expected yield of 5.3mCi/µAh. The system is able to deliver reliable and reproducible radioactivity for patients and can be operated up to 1500µAh before preventive maintenance is due. Key steps in designing the (81)Rb target for selected energy ranges are reported here.

  8. Modification of a force plate system for equine gait analysis on hard road surfaces: a technical note.

    PubMed

    Wilson, A M; Pardoe, C H

    2001-04-01

    Studies on horseshoe materials have been limited to the analysis of kinematic data to determine slip times and distances, since equine force plate analysis is traditionally undertaken on a rubber-surfaced force plate. The purpose of this study was to modify a force plate for measuring ground reaction forces on a road surface and report preliminary data from the system. A steel-reinforced concrete top plate of 18 mm thickness and mass 23 kg, and a 12 mm thick top plate of 6 mm bituminous macadam wear coat (road surface) contained in a tray constructed of 2 mm thick steel, mass 21 kg, were constructed. The top plates were bolted to a forceplate and resonance frequency of the force plate top plate combinations were 278 Hz (concrete) and 218 Hz (roadway), respectively. Simultaneous kinetic and kinematic data were collected while a horse, shod in steel horseshoes, was trotted over the concrete-topped force plate until 8 foot placements were recorded. The foot slipped for mean +/- s.d. 35 +/- 7 mm in 29 +/- 8 ms after impact and both Fy and Fz increased during foot slip. The ratio of Fy/Fz during slip was relatively constant at 0.56 +/- 0.05. The top plates have proved resilient in use and have withstood a variety of horseshoe designs and tungsten-capped stud nails with minimal damage. They enable measurement of ground reaction forces on hard surfaces and the calculation of the energy dissipated via foot slippage by integration of the craniocaudal force-foot position data after impact.

  9. FBG sensor array-based-low speed impact localization system on composite plate

    NASA Astrophysics Data System (ADS)

    Sai, Yaozhang; Jiang, Mingshun; Sui, Qingmei; Lu, Shizeng; Jia, Lei

    2016-03-01

    A fiber Bragg grating (FBG) sensors-based impact localization system on composite structure and a novel localization algorithm independent of wave velocity were proposed. Six FBG sensors constitute two isosceles right triangle FBG arrays. Impact signals were detected by a high-speed FBG interrogation system. Morlet wavelet transform was employed to extract time differences of impact signals. The straight lines equations, which are through impact source and FBG sensors of right-angled vertices of FBG arrays, can be obtained by the time differences. The coordinate of impact source is the intersection of straight lines. Testing experiments were carried out on composite plate within 400 mm × 400 mm monitor area. The experimental results showed that the maximum and average errors were 20.92 and 8.67 mm, respectively. This article provides a simple and stable impact source localization system independent of wave velocity.

  10. Cooled electronic system with liquid-cooled cold plate and thermal spreader coupled to electronic component

    DOEpatents

    Chainer, Timothy J.; Graybill, David P.; Iyengar, Madhusudan K.; Kamath, Vinod; Kochuparambil, Bejoy J.; Schmidt, Roger R.; Steinke, Mark E.

    2016-04-05

    Apparatus and method are provided for facilitating cooling of an electronic component. The apparatus includes a liquid-cooled cold plate and a thermal spreader associated with the cold plate. The cold plate includes multiple coolant-carrying channel sections extending within the cold plate, and a thermal conduction surface with a larger surface area than a surface area of the component to be cooled. The thermal spreader includes one or more heat pipes including multiple heat pipe sections. One or more heat pipe sections are partially aligned to a first region of the cold plate, that is, where aligned to the surface to be cooled, and partially aligned to a second region of the cold plate, which is outside the first region. The one or more heat pipes facilitate distribution of heat from the electronic component to coolant-carrying channel sections of the cold plate located in the second region of the cold plate.

  11. Cooled electronic system with liquid-cooled cold plate and thermal spreader coupled to electronic component

    DOEpatents

    Chainer, Timothy J.; Graybill, David P.; Iyengar, Madhusudan K.; Kamath, Vinod; Kochuparambil, Bejoy J.; Schmidt, Roger R.; Steinke, Mark E.

    2016-08-09

    Apparatus and method are provided for facilitating cooling of an electronic component. The apparatus includes a liquid-cooled cold plate and a thermal spreader associated with the cold plate. The cold plate includes multiple coolant-carrying channel sections extending within the cold plate, and a thermal conduction surface with a larger surface area than a surface area of the component to be cooled. The thermal spreader includes one or more heat pipes including multiple heat pipe sections. One or more heat pipe sections are partially aligned to a first region of the cold plate, that is, where aligned to the surface to be cooled, and partially aligned to a second region of the cold plate, which is outside the first region. The one or more heat pipes facilitate distribution of heat from the electronic component to coolant-carrying channel sections of the cold plate located in the second region of the cold plate.

  12. Flat-plate boiloff calorimeters for testing of thermal insulation systems

    NASA Astrophysics Data System (ADS)

    Fesmire, J. E.; Johnson, W. L.; Kelly, A. O.; Meneghelli, B. J.; Swanger, A. M.

    2015-12-01

    Cryostats have been developed and standardized for laboratory testing of thermal insulation systems in a flat-plate configuration. Boiloff calorimetry is the measurement principle for determining the effective thermal conductivity (ke) and heat flux (q) of test specimens under a wide range of actual conditions. Cryostat-500 is thermally guarded to measure absolute thermal performance when calibrated with a known reference via an adjustable-edge guard ring. With liquid nitrogen as the energy meter, the cold boundary temperature can be adjusted to any temperature between 77 K and approximately 300 K by the interposition of a thermal resistance layer between the cold mass and the specimen. A low thermal conductivity suspension system has compliance rods that adjust for specimen thickness and compression force. Material type, thickness, density, flatness, compliance, outgassing, and temperature sensor placement are important test considerations, and edge effects and calibration techniques for the apparatus are crucial. Over the full vacuum pressure range, the thermal performance capability is nearly four orders of magnitude. The horizontal configuration provides key advantages over the vertical cylindrical cryostats for testing at ambient pressure conditions. Cryostat-500’s design and test methods, other flat-plate boiloff calorimeters, and results for select thermal insulation materials (composites, foams, aerogels) are discussed.

  13. A serial sample loading system: interfacing multiwell plates with microfluidic devices.

    PubMed

    Rane, Tushar D; Zec, Helena C; Wang, Tza-Huei

    2012-10-01

    There is an increasing demand for novel high-throughput screening (HTS) technologies in the pharmaceutical and biotechnological industries. The robotic sample-handling techniques currently used in these industries, although fast, are still limited to operating in multiwell plates with the sample volumes per reaction in the microliter regime. Digital microfluidics offers an alternative for reduction in sample volume consumption for HTS but lacks a reliable technique for transporting a large number of samples to the microfluidic device. In this report, we develop a technique for serial delivery of sample arrays to a microfluidic device from multiwell plates, through a single sample inlet. Under this approach, a serial array of sample plugs, separated by an immiscible carrier fluid, is loaded into a capillary and delivered to a microfluidic device. Similar approaches have been attempted in the past, however, either with a slower sample loading device such as a syringe pump or vacuum-based sample loading with limited driving pressure. We demonstrated the application of our positive-pressure-based serial sample loading (SSL) system to load a series of sample plugs into a capillary. The adaptability of the SSL system to generate sample plugs with a variety of volumes in a predictable manner was also demonstrated.

  14. A Serial Sample Loading System: Interfacing Multi-well plates with Microfluidic Devices

    PubMed Central

    Rane, Tushar D.; Zec, Helena; Wang, Jeff Tza-Huei

    2013-01-01

    There is an increasing demand for novel high-throughput screening (HTS) technologies in the pharmaceutical and biotechnological industries. The robotic sample handling techniques currently used in these industries, although fast, are still limited to operating in multi-well plates with the sample volumes per reaction in the microliter regime. Digital microfluidics offers an alternative for reduction in sample volume consumption for HTS but lacks a reliable technique for transporting large number of samples to the microfluidic device. In this report, we develop a technique for serial delivery of sample arrays to a microfluidic device from multi-well plates, through a single sample inlet. Under this approach, a serial array of sample plugs, separated by an immiscible carrier fluid, is loaded into a capillary and delivered to a microfluidic device. Similar approaches have been attempted in the past, however, either with a slower sample loading device like syringe pump or vacuum based sample loading with limited driving pressure. We demonstrated the application of our positive pressure based ‘Serial Sample Loading’ (SSL) system to load a series of sample plugs into a capillary. The adaptability of the SSL system to generate sample plugs with a variety of volumes in a predictable manner was also demonstrated. PMID:22885789

  15. Stand-alone flat-plate photovoltaic power systems: System sizing and life-cycle costing methodology for Federal agencies

    NASA Technical Reports Server (NTRS)

    Borden, C. S.; Volkmer, K.; Cochrane, E. H.; Lawson, A. C.

    1984-01-01

    A simple methodology to estimate photovoltaic system size and life-cycle costs in stand-alone applications is presented. It is designed to assist engineers at Government agencies in determining the feasibility of using small stand-alone photovoltaic systems to supply ac or dc power to the load. Photovoltaic system design considerations are presented as well as the equations for sizing the flat-plate array and the battery storage to meet the required load. Cost effectiveness of a candidate photovoltaic system is based on comparison with the life-cycle cost of alternative systems. Examples of alternative systems addressed are batteries, diesel generators, the utility grid, and other renewable energy systems.

  16. A plate-driven model for enigmatic volcanic history of the Cascades-Yellowstone System

    NASA Astrophysics Data System (ADS)

    Szwaja, S.; Kincaid, C. R.; Druken, K. A.; MacDougall, J.

    2013-12-01

    The Cascades subduction system in the Pacific Northwest (USA) represents a complex tectonic setting, where rollback subduction of the Juan de Fuca plate beneath the North American plate, back-arc extension, and a possible mantle plume have been proposed to explain the complicated volcanic trends observed over the past 20 Ma. Plume and non-plume models have been developed to reconcile the voluminous Columbia River/Steens Flood Basalts (CSFB) (~20 Ma), the age progressive (15 Ma to present) Snake River Plain (SRP) that terminates at Yellowstone and the opposite, or westward trending High Lava Plains (HLP) volcanic track of eastern/central Oregon. We present results from laboratory experiments designed to test a plate-driven model for reproducing gross spatial-temporal characteristics of these three magmatic features. Models use a glucose fluid with temperature dependent viscosity in representing Earth's mantle and continuous rubber belts that kinematically reproduce subduction trends for the Cascades system. Experiments begin at 20 Ma with a volume of mantle residuum in the Cascades wedge that is elongated and restricted in the trench-parallel and trench-normal directions, respectively. The underlying assumption is that residuum was created in the wedge during an earlier plate steepening event that caused the flood basalts. Our models characterize dispersion patterns for the melt residuum material as it deforms within four-dimensional wedge circulation fields driven by rollback subduction (e.g. with a translational component of motion). Results show that residuum viscosity, relative to the ambient fluid, determines whether anomalous fluid can evolve to a morphology that matches the SRP/HLP tracks over ~15-20Ma. A weak residuum (e.g. retained partial melt) deforms over this time scale from the initial north-south oriented feature to an east-west trending morphology that is thin in both depth and north-south extent, material initially beneath CSFB is offset to the

  17. Compact three-dimensional head-mounted display system with Savart plate.

    PubMed

    Lee, Chang-Kun; Moon, Seokil; Lee, Seungjae; Yoo, Dongheon; Hong, Jong-Young; Lee, Byoungho

    2016-08-22

    We propose three-dimensional (3D) head-mounted display (HMD) providing multi-focal and wearable functions by using polarization-dependent optical path switching in Savart plate. The multi-focal function is implemented as micro display with high pixel density of 1666 pixels per inches is optically duplicated in longitudinal direction according to the polarization state. The combination of micro display, fast switching polarization rotator and Savart plate retains small form factor suitable for wearable function. The optical aberrations of duplicated panels are investigated by ray tracing according to both wavelength and polarization state. Astigmatism and lateral chromatic aberration of extraordinary wave are compensated by modification of the Savart plate and sub-pixel shifting method, respectively. To verify the feasibility of the proposed system, a prototype of the HMD module for monocular eye is implemented. The module has the compact size of 40 mm by 90 mm by 40 mm and the weight of 131 g with wearable function. The micro display and polarization rotator are synchronized in real-time as 30 Hz and two focal planes are formed at 640 and 900 mm away from eye box, respectively. In experiments, the prototype also provides augmented reality function by combining the optically duplicated panels with a beam splitter. The multi-focal function of the optically duplicated panels without astigmatism and color dispersion compensation is verified. When light field optimization for two additive layers is performed, perspective images are observed, and the integration of real world scene and high quality 3D images is confirmed.

  18. New Whole-House Solutions Case Study: Advanced Extended Plate and Beam Wall System in a Cold-Climate House

    SciTech Connect

    2016-02-10

    A zero energy ready home was recently completed that features an innovative wall system. This highly insulated (high-R) light-frame wall system, called the extended plate and beam, is for use above grade in residential buildings. The Building America research team Home Innovation Research Labs featured this system in a new construction test house.

  19. Wave-plate structures, power selective optical filter devices, and optical systems using same

    SciTech Connect

    Koplow, Jeffrey P

    2012-07-03

    In an embodiment, an optical filter device includes an input polarizer for selectively transmitting an input signal. The device includes a wave-plate structure positioned to receive the input signal, which includes first and second substantially zero-order, zero-wave plates arranged in series with and oriented at an angle relative to each other. The first and second zero-wave plates are configured to alter a polarization state of the input signal passing in a manner that depends on the power of the input signal. Each zero-wave plate includes an entry and exit wave plate each having a fast axis, with the fast axes oriented substantially perpendicular to each other. Each entry wave plate is oriented relative to a transmission axis of the input polarizer at a respective angle. An output polarizer is positioned to receive a signal output from the wave-plate structure and selectively transmits the signal based on the polarization state.

  20. The `Plate-Like' Subsidence of the East Pacific Rise - South Pacific Superswell System

    NASA Astrophysics Data System (ADS)

    Hillier, J. K.; Watts, T. B.

    2003-12-01

    suggests to us a common causal mechanism that links processes acting at the Superswell with those at the EPR. Previous studies at the EPR show asymmetry in seafloor subsidence and other observables such as seismic velocity and electrical conductivity which have been interpreted in terms of an across-axis temperature gradient, possibly sustained by a flow of hotter material from the west. Thus, a lateral temperature gradient may exist across the entire EPR-Superswell system. We have tested this hypothesis using a model in which the isostatic and thermal effects of a lateral sub-lithospheric temperature gradient are allowed to perturb the subsidence of a standard plate. Our preferred model has a depth of isostatic compensation of 320km and a linear temperature gradient of only 0.014oC/km. Such a model better explains the regional seafloor depths of the EPR-Superswell system than published cooling plate models and is in accord with constraints from elastic thickness, heat flow, seismic tomographic and long-wavelength gravity anomalies.

  1. Validation of a sensitive and automated 96-well solid-phase extraction liquid chromatography-tandem mass spectrometry method for the determination of desloratadine and 3-hydroxydesloratadine in human plasma.

    PubMed

    Yang, Liyu; Clement, Robert P; Kantesaria, Bhavna; Reyderman, Larisa; Beaudry, Francis; Grandmaison, Charles; Di Donato, Lorella; Masse, Robert; Rudewicz, Patrick J

    2003-07-25

    To support clinical development, a liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed and validated for the determination of desloratadine (descarboethoxyloratadine) and 3-OH desloratadine (3-hydroxydescarboethoxyloratadine) concentrations in human plasma. The method consisted of automated 96-well solid-phase extraction for sample preparation and liquid chromatography/turbo ionspray tandem mass spectrometry for analysis. [2H(4)]Desloratadine and [2H(4)]3-OH desloratadine were used as internal standards (I.S.). A quadratic regression (weighted 1/concentration(2)) gave the best fit for calibration curves over the concentration range of 25-10000 pg/ml for both desloratadine and 3-OH desloratadine. There was no interference from endogenous components in the blank plasma tested. The accuracy (%bias) at the lower limit of quantitation (LLOQ) was -12.8 and +3.4% for desloratadine and 3-OH desloratadine, respectively. The precision (%CV) for samples at the LLOQ was 15.1 and 10.9% for desloratadine and 3-OH desloratadine, respectively. For quality control samples at 75, 1000 and 7500 pg/ml, the between run %CV was

  2. Validation and application of a liquid chromatography-tandem mass spectrometric method for the determination of GDC-0834 and its metabolite in human plasma using semi-automated 96-well protein precipitation.

    PubMed

    Shin, Young G; Jones, Steve A; Murakami, Stan C; Liu, Lichuan; Wong, Harvey; Buonarati, Michael H; Hop, Cornelis E C A

    2012-11-01

    A liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of GDC-0834 and its amide hydrolysis metabolite (M1) in human plasma to support clinical development. The method consisted of semi-automated 96-well protein precipitation extraction for sample preparation and LC-MS/MS analysis in positive ion mode using TurboIonSpray® for analysis. D6-GDC-0834 and D6-M1 metabolite were used as internal standards. A linear regression (weighted 1/concentration(2) ) was used to fit calibration curves over the concentration range of 1 - 500 ng/mL for both GDC-0834 and M1 metabolite. The accuracy (percentage bias) at the lower limit of quantitation (LLOQ) was 5.20 and 0.100% for GDC-0834 and M1 metabolite, respectively. The precision (CV) for samples at the LLOQ was 3.13-8.84 and 5.20-8.93% for GDC-0834 and M1 metabolite, respectively. For quality control samples at 3, 200 and 400 ng/mL, the between-run CV was ≤ 7.38% for GDC-0834 and ≤ 8.20% for M1 metabolite. Between run percentage bias ranged from -2.76 to 6.98% for GDC-0834 and from -6.73 to 2.21% for M1 metabolite. GDC-0834 and M1 metabolite were stable in human plasma for 31 days at -20 and -70°C. This method was successfully applied to support a GDC-0834 human pharmacokinetic-based study.

  3. Comparative Evaluation of Colorimetric Microtiter Plate Systems for Detection of Herpes Simplex Virus in Cerebrospinal Fluid

    PubMed Central

    Tang, Yi-Wei; Rys, Paul N.; Rutledge, Barbara J.; Mitchell, P. Shawn; Smith, Thomas F.; Persing, David H.

    1998-01-01

    In the past few years, application of the PCR to the detection of herpes simplex virus (HSV) DNA in the cerebrospinal fluid (CSF) from patients with encephalitis and meningitis has become standard laboratory practice. However, from an operational perspective, the true diagnostic value of PCR in this setting is yet to be realized because most laboratories subject the amplification products to lengthy probe hybridization procedures by Southern blotting. As alternatives to Southern blotting, we evaluated colorimetric microtiter plate (MTP) systems from ViroMed Laboratories, Inc. (PrimeCapture), CPG, Inc. (Quanti-PATH), and Incstar Corp. (GEN-ETI-K), in addition to a system developed at the Mayo Clinic with the PCR ELISA system (Boehringer Mannheim Corp.). We tested PCR products from 86 clinical CSF specimens submitted to our Molecular Microbiology Laboratory. The CSF specimens used had to have sufficient volume for comparative analysis. By conventional Southern blotting methods, 54 were positive and 32 were negative for HSV DNA. Compared with Southern blotting, the sensitivity and specificity were 63.0 and 100.0%, respectively, for the PrimeCapture system, 98.2 and 96.9%, respectively, for the Quanti-PATH system, 98.2 and 100.0%, respectively, for the GEN-ETI-K system, and 100.0 and 96.9%, respectively, for the Mayo system. All four MTP systems had turnaround times 12 to 24 h less than that for Southern blotting. There were no significant differences in costs or technologist time between the Mayo system and Southern blotting. Other features of the Mayo system include type-specific genotypic identification of HSV and the potential for determination of drug resistance by DNA sequencing. Overall, we found that colorimetric MTP systems were likely to improve test turnaround times and patient care at no additional cost. PMID:9705419

  4. Mantle flow geometry from ridge to trench beneath the Gorda-Juan de Fuca plate system

    NASA Astrophysics Data System (ADS)

    Martin-Short, Robert; Allen, Richard M.; Bastow, Ian D.; Totten, Eoghan; Richards, Mark A.

    2015-12-01

    Tectonic plates are underlain by a low-viscosity mantle layer, the asthenosphere. Asthenospheric flow may be induced by the overriding plate or by deeper mantle convection. Shear strain due to this flow can be inferred using the directional dependence of seismic wave speeds--seismic anisotropy. However, isolation of asthenospheric signals is challenging; most seismometers are located on continents, whose complex structure influences the seismic waves en route to the surface. The Cascadia Initiative, an offshore seismometer deployment in the US Pacific Northwest, offers the opportunity to analyse seismic data recorded on simpler oceanic lithosphere. Here we use measurements of seismic anisotropy across the Juan de Fuca and Gorda plates to reconstruct patterns of asthenospheric mantle shear flow from the Juan de Fuca mid-ocean ridge to the Cascadia subduction zone trench. We find that the direction of fastest seismic wave motion rotates with increasing distance from the mid-ocean ridge to become aligned with the direction of motion of the Juan de Fuca Plate, implying that this plate influences mantle flow. In contrast, asthenospheric mantle flow beneath the Gorda Plate does not align with Gorda Plate motion and instead aligns with the neighbouring Pacific Plate motion. These results show that asthenospheric flow beneath the small, slow-moving Gorda Plate is controlled largely by advection due to the much larger, faster-moving Pacific Plate.

  5. Design study of multi-imaging plate system for BNCT irradiation field at Kyoto university reactor.

    PubMed

    Tanaka, Kenichi; Sakurai, Yoshinori; Kajimoto, Tsuyoshi; Tanaka, Hiroki; Takata, Takushi; Endo, Satoru

    2016-09-01

    The converter configuration for a multi-imaging plate system was investigated for the application of quality assurance in the irradiation field profile for boron neutron capture therapy. This was performed by the simulation calculation using the PHITS code in the fields at the Heavy Water Neutron Irradiation Facility of Kyoto University Reactor. The converter constituents investigated were carbon for gamma rays, and polyethylene with and without LiF at varied (6)Li concentration for thermal, epithermal, and fast neutrons. Consequently, potential combinations of the converters were found for two components, gamma rays and thermal neutrons, for the standard thermal neutron mode and three components of gamma rays, epithermal neutrons, and thermal or fast neutrons, for the standard mixed or epithermal neutron modes, respectively.

  6. Constraints on Pacific plate kinematics and dynamics with global positioning system measurements

    NASA Technical Reports Server (NTRS)

    Dixon, T. H.; Golombek, M. P.; Thornton, C. L.

    1985-01-01

    A measurement program designed to investigate kinematic and dynamic aspects of plate tectonics in the Pacific region by means of satellite observations is proposed. Accuracy studies are summarized showing that for short baselines (less than 100 km), the measuring accuracy of global positioning system (GPS) receivers can be in the centimeter range. For longer baselines, uncertainty in the orbital ephemerides of the GPS satellites could be a major source of error. Simultaneous observations at widely (about 300 km) separated fiducial stations over the Pacific region, should permit an accuracy in the centimeter range for baselines of up to several thousand kilometers. The optimum performance level is based on the assumption of that fiducial baselines are known a priori to the centimeter range. An example fiducial network for a GPS study of the South Pacific region is described.

  7. Objective evaluation of interior trim effects on sound quality and noise reduction of a coupled plate cavity system

    NASA Astrophysics Data System (ADS)

    Egab, Laith; Wang, Xu

    2016-03-01

    In this study, the impedance mobility and psychoacoustic analysis methods are combined to develop a structural-acoustic model of a plate-cavity coupling system. The objective is to evaluate the effect of interior trim materials on sound loudness and sharpness of a plate-cavity coupling system. The impedance mobility method is applied to calculate the pressure frequency responses of the interior acoustic field for the plate-cavity coupling system. The sound pressure results calculated by the impedance mobility method are then directly used to calculate the psychoacoustic metrics using psychoacoustic analysis method. A good agreement was found between the experimental and analytical results. The results show that the interior trim has a large influence on the distribution of the sound loudness and sharpness inside the cavity in the middle and high frequency ranges.

  8. Progress towards a 256 channel multi-anode microchannel plate photomultiplier system with picosecond timing

    PubMed Central

    Lapington, J.S.; Ashton, T.J.R.; Ross, D.; Conneely, T.

    2012-01-01

    Despite the rapid advances in solid state technologies such as the silicon photomultiplier (SiPM), microchannel plate (MCP) photomultipliers still offer a proven and practical technological solution for high channel count pixellated photon-counting systems with very high time resolution. We describe progress towards a 256 channel optical photon-counting system using CERN-developed NINO and HTDC ASICs, and designed primarily for time resolved spectroscopy in life science applications. Having previously built and demonstrated a 18 mm diameter prototype tube with an 8×8 channel readout configuration and <43 ps rms single photon timing resolution, we are currently developing a 40 mm device with a 32×32 channel readout. Initially this will be populated with a 256 channel electronics system comprising four sets of modular 64 channel preamplifier/discriminator, and time-to-digital converter units, arranged in a compact three dimensional configuration. We describe the detector and electronics design and operation, and present performance measurements from the 256 channel development system. We discuss enhancements to the system including higher channel count and the use of application specific on-board signal processing capabilities. PMID:25843997

  9. Extended Plate and Beam Wall System: Concept Investigation and Initial Evaluation

    SciTech Connect

    Wiehagen, J.; Kochkin, V.

    2015-08-01

    A new and innovative High-R wall design, referred to as the Extended Plate & Beam (EP&B), is under development. The EP&B system uniquely integrates foam sheathing insulation with wall framing such that wood structural panels are installed exterior of the foam sheathing, enabling the use of standard practices for installation of drainage plane, windows and doors, claddings, cavity insulation, and the standard exterior foam sheathing installation approach prone to damage of the foam during transportation of prefabricated wall panels. As part of the ongoing work, the EP&B wall system concept has undergone structural verification testing and has been positively vetted by a group of industry stakeholders. Having passed these initial milestone markers, the advanced wall system design has been analyzed to assess cost implications relative to other advanced wall systems, undergone design assessment to develop construction details, and has been evaluated to develop representative prescriptive requirements for the building code. This report summarizes the assessment steps conducted to-date and provides details of the concept development.

  10. Fluid-structure interaction study of the splitter plate in a TBCC exhaust system during mode transition phase

    NASA Astrophysics Data System (ADS)

    Guo, Shuai; Xu, Jinglei; Mo, Jianwei; Gu, Rui; Pang, Lina

    2015-07-01

    Splitter plate plays an important role in a turbine-based combined-cycle (TBCC) exhaust system during the mode transition phase when turbojet engine and ramjet engine operate simultaneously. Dissimilar pressure distribution on both sides of the plate has a potential origin in the aeroelastic coupling, which is an interesting topic while few research works have devoted to that aspect. To better understand the aeroelastic behavior of the plate and the corresponding dynamic flow features, an integrated fluid-structure interaction simulation is conducted under one particular operation condition during mode transition phase in the TBCC exhaust system. A finite-volume-based CFD solver FLUENT is adopted to solve the unsteady Reynolds average Navier-Stokes equations. ABAQUS, a finite-element-method-based CSD solver, is employed to compute the plate elastic deformation. A two-way interaction between the fluid and the structure is accomplished by the mesh-based parallel-code coupling interface (MpCCI) in a loosely-coupled manner. The accuracy of the coupling procedure is validated for the flutter of a flat plate in supersonic flow. Then, features of steady flow field of the TBCC exhaust system are discussed, followed by the investigation of the aeroelastic phenomenon of the splitter plate and the evolution process of the flow field pattern. Finally, performances variation of the exhaust system is obtained and discussed. The results show that the plate vibrates with decaying amplitude and reaches a dynamic stable state eventually. The thrust, lift and pitch moment of the TBCC exhaust system are increased by 0.68%, 2.82% and 5.86%, respectively, compared with the corresponding values in steady state which does not take into account the fluid-structure interaction effects. The analysis reveals the importance of considering the fluid-structure interaction effects in designing the splitter plate in the TBCC exhaust system and demonstrates the availability of the present coupled

  11. Coefficient of Variation Estimates for the Plate Boundary Fault System of California

    NASA Astrophysics Data System (ADS)

    Biasi, G. P.; Scharer, K. M.

    2015-12-01

    The number of high-quality paleoseismic records on major strike-slip faults of California has increased in recent years to the point that patterns in earthquake recurrence are emerging. The degree of predictability in time intervals between ground-rupturing earthquakes can be measured by the CoV (coefficient of variation). The CoV approximately normalizes for mean recurrence, and is thus useful to isolate the temporal variability of earthquake records. CoV estimates are themselves uncertain because input dates are actually probability distributions and because paleoseismic records are short and not necessarily representative samples from the underlying recurrence distribution. Radiocarbon dating uncertainty can be incorporated by sampling from event PDFs and compiling sample CoV estimates. Uncertainty due to the brevity of the site event record is larger, and neglect of it can lead to improbable estimates. Long records are now available on the San Andreas and San Jacinto faults in Southern California, and the San Andreas and Hayward faults in northern California. These faults accommodate most of the Pacific-North American relative plate motion in their respective regions. CoV estimates from sites with 8 or more events cluster around 0.63, but are as low as 0.4 for the southern Hayward fault. Sites with fewer events give similar estimates, though with lower resolution. The one prominent outlier, Burro Flats, with a CoV near 1.0, is in a region of severe fault complexity and rapid fault-normal compression. Quasi-periodic recurrence is emerging as a general property for these plate boundary faults. Some individual site records allow that, at low probabilities, recurrence could be random in time. When the ensemble is considered together, however, it is improbable that we would see the observed degree of agreement among boundary fault paleoseismic records; the more likely explanation is that quasi-periodic recurrence is a real property of the boundary fault system.

  12. An improved plating process

    NASA Technical Reports Server (NTRS)

    Askew, John C.

    1994-01-01

    An alternative to the immersion process for the electrodeposition of chromium from aqueous solutions on the inside diameter (ID) of long tubes is described. The Vessel Plating Process eliminates the need for deep processing tanks, large volumes of solutions, and associated safety and environmental concerns. Vessel Plating allows the process to be monitored and controlled by computer thus increasing reliability, flexibility and quality. Elimination of the trivalent chromium accumulation normally associated with ID plating is intrinsic to the Vessel Plating Process. The construction and operation of a prototype Vessel Plating Facility with emphasis on materials of construction, engineered and operational safety and a unique system for rinse water recovery are described.

  13. Image-based fluidic sorting system for automated Zebrafish egg sorting into multiwell plates.

    PubMed

    Graf, Siegfried F; Hötzel, Sebastian; Liebel, Urban; Stemmer, Andreas; Knapp, Helmut F

    2011-04-01

    The global demand for the reduction of animal testing has led to the emergence of Zebrafish eggs/larvae as model organisms to replace current adult animal testing in, for example, toxicity testing. Because of the egg size (diameter 1.6mm) and the relatively easy maintenance of Zebrafish farms the eggs also offer high-throughput screening (HTS). However, the current bottleneck for HTS is the cost-efficient placing of individual organisms into single wells of a multiwell plate (MWP). The system presented here is capable of storing, sorting, and placing individual organisms in a highly reproducible manner. In about 11 min a complete 96-MWP is filled, which corresponds to about 8 sec per egg. The survival rate of fertilized transgenic and wild-type eggs was comparable to the one of the control (control 6.7%, system 7.6%). Furthermore, it was also possible to place dechorionated eggs into individual wells. The results demonstrate that the cost efficient system works gentle and reliable enough to disburden scientists from the exhausting and monotonous job of placing single eggs into single wells, such that they can concentrate on the scientific aspects of their experiments and create results with a higher statistical relevance.

  14. Plate motion

    SciTech Connect

    Gordon, R.G. )

    1991-01-01

    The motion of tectonic plates on the earth is characterized in a critical review of U.S. research from the period 1987-1990. Topics addressed include the NUVEL-1 global model of current plate motions, diffuse plate boundaries and the oceanic lithosphere, the relation between plate motions and distributed deformations, accelerations and the steadiness of plate motions, the distribution of current Pacific-North America motion across western North America and its margin, plate reconstructions and their uncertainties, hotspots, and plate dynamics. A comprehensive bibliography is provided. 126 refs.

  15. Lameness scoring system for dairy cows using force plates and artificial intelligence.

    PubMed

    Ghotoorlar, S Mokaram; Ghamsari, S Mehdi; Nowrouzian, I; Ghotoorlar, S Mokaram; Ghidary, S Shiry

    2012-02-04

    Lameness scoring is a routine procedure in dairy industry to screen the herds for new cases of lameness. Subjective lameness scoring, which is the most popular lameness detection and screening method in dairy herds, has several limitations. They include low intra-observer and inter-observer agreement and the discrete nature of the scores which limits its usage in monitoring the lameness. The aim of this study is to develop an automated lameness scoring system comparable with conventional subjective lameness scoring by means of artificial neural networks. The system is composed of four balanced force plates installed in a hoof-trimming box. A group of 105 dairy cows was used for the study. Twenty-three features extracted from ground reaction force (GRF) data were used in a computer training process which was performed on 60 per cent of the data. The remaining 40 per cent of the data were used to test the trained system. Repeatability of the lameness scoring system was determined by GRF samples from 25 cows, captured at two different times from the same animals. The mean sd was 0.31 and the mean coefficient of variation was 14.55 per cent, which represents a high repeatability in comparison with subjective vision-based scoring methods. Although the highest sensitivity and specificity values were seen in locomotion score groups 1 and 4, the automatic lameness system was both sensitive and specific in all groups. The sensitivity and specificity were higher than 72 per cent in locomotion score groups 1 to 4, and it was 100 per cent specific and 50 per cent sensitive for group 5.

  16. Chlorine isotope geochemistry of Icelandic thermal fluids: Implications for geothermal system behavior at divergent plate boundaries

    NASA Astrophysics Data System (ADS)

    Stefánsson, Andri; Barnes, Jaime D.

    2016-09-01

    The chlorine isotope composition of thermal fluids from Iceland were measured in order to evaluate the source of chlorine and possible chlorine isotope fractionation in geothermal systems at divergent plate boundaries. The geothermal systems studied have a wide range of reservoir temperatures from 40 to 437 °C and in-situ pH of 6.15 to 7.15. Chlorine concentrations range from 5.2 to 171 ppm and δ37 Cl values are -0.3 to + 2.1 ‰ (n = 38). The δ37 Cl values of the thermal fluids are interpreted to reflect the source of the chlorine in the fluids. Geothermal processes such as secondary mineral formation, aqueous and vapor speciation and boiling were found to have minimal effects on the δ37 Cl values. However, further work is needed on incorporation of Cl into secondary minerals and its effect on Cl isotope fractionation. Results of isotope geochemical modeling demonstrate that the range of δ37 Cl values documented in the natural thermal fluids can be explained by leaching of the basaltic rocks by meteoric source water under geothermal conditions. Magmatic gas partitioning may also contribute to the source of Cl in some cases. The range of δ37 Cl values of the fluids result mainly from the large range of δ37 Cl values observed for Icelandic basalts, which range from -0.6 to + 1.2 ‰.

  17. Mixing at the microscale: Power input in shaken microtiter plates.

    PubMed

    Dürauer, Astrid; Hobiger, Stefanie; Walther, Cornelia; Jungbauer, Alois

    2016-12-01

    Power input and local energy dissipation are crucial parameters for the engineering characterization of mixing and fluid dynamics at the microscale. Since hydrodynamic stress is solely dependent on the maximum power input, we adapted the clay/polymer method to obtain flock destruction kinetics in six-, 24-, and 96-well microtiter plates on orbital shakers. We also determined the specific power input using calorimetry and found that the power input is at the same order of magnitude for the six- and 96-well plates and the laboratory-scale stirred tank reactor, with 40 to 90 W/m(3) (Re' = 180 to 440), 40 to 140 W/m3 (Re' = 320 to 640), and 30 to 50 W/m(3) (Re = 4000 to 8500), respectively. All of these values are significantly below 450 to 2100 W/m(3) determined for the pilot-scale reactor. The hydrodynamic stress differs significantly between the different formats of MTPs, as the 96-well plates showed very low shear stress on the shaker with a shaking amplitude of 3 mm. Thus, the transfer of mixing conditions from the microtiter plate to small-scale and pilot-scale reactors must be undertaken with care. Our findings, especially the power input determined by the calorimetric method, show that the hydrodynamic conditions in laboratory- and pilot-scale reactors cannot be reached.

  18. Clinical results of using the proximal humeral internal locking system plate for internal fixation of displaced proximal humeral fractures.

    PubMed

    Norouzi, Masoud; Naderi, Mohammad Nasir; Komasi, Mehdi Hemmati; Sharifzadeh, Seyyed Reza; Shahrezaei, Mostafa; Eajazi, Alireza

    2012-05-01

    Proximal humerus fractures are accounting for 4-5% of all fractures with increasing incidence. Proximal Humeral Internal Locking System (PHILOS) plate is a new plate which permits early mobility and lowers the risk of complications. The aim of this study was to evaluate the functional outcome and the complication rate after using this plate. Between 2006-2008, 37 patients with displaced 2-, 3-, and 4-part fractures of the proximal humerus underwent surgery using PHILOS plate. The mean range of follow-up was 12 months. Twenty patients were aged 60 years and younger, and 17 were aged older than 60 years. The average American Shoulder and Elbow Surgeons (ASES) score at the final follow-up was 77.62. According to Michener and colleagues classification, 5.4% of patients had an excellent outcome, 72.9% were minimally functionally limited, 16.2% were moderately functionally limited, and 5.4% were maximally functionally limited. The average ASES score between patients 60 years and older and those 60 years and younger was not different significantly. One patient developed avascular necrosis of the humeral head, 2 patients developed an infection, and no patients developed a nonunion. Fixation with PHILOS plate can be considered a good method with high union rates for this kind of fracture, especially in the older population with osteoporotic bone.

  19. An Expert System to Analyze Homogeneity in Fuel Element Plates for Research Reactors

    SciTech Connect

    Tolosa, S.C.; Marajofsky, A.

    2004-10-06

    In the manufacturing control of Fuel Element Plates for Research Reactors, one of the problems to be addressed is how to determine the U-density homogeneity in a fuel plate and how to obtain qualitative and quantitative information in order to establish acceptance or rejection criteria for such, as well as carrying out the quality follow-up. This paper is aimed at developing computing software which implements an Unsupervised Competitive Learning Neural Network for the acknowledgment of regions belonging to a digitalized gray scale image. This program is applied to x-ray images. These images are generated when the x-ray beams go through a fuel plate of approximately 60 cm x 8 cm x 0.1 cm thick. A Nuclear Fuel Element for Research Reactors usually consists of 18 to 22 of these plates, positioned in parallel, in an arrangement of 8 x 7 cm. Carrying out the inspection of the digitalized x-ray image, the neural network detects regions with different luminous densities corresponding to U-densities in the fuel plate. This is used in quality control to detect failures and verify acceptance criteria depending on the homogeneity of the plate. This modality of inspection is important as it allows the performance of non-destructive measurements and the automatic generation of the map of U-relative densities of the fuel plate.

  20. On the Analysis of Old Objective-Prism Plate Spectra with Modern Systems

    NASA Astrophysics Data System (ADS)

    Rossi, C.; Polcaro, V. F.; Viotti, R. F.; Filippi, A.; Pedicelli, S.; Omizzolo, A.

    2004-07-01

    Objective prism (OP) spectroscopic plates collected with Schmidt Telescopes are a heritage of the pre-electronic Astronomy which may still contain useful data for statistical researches, and precious information on unrecorded peculiar events as well. Modern imaging processing techniques may allow to extract rapidly the massive amount of information included in a single plate. We present the results of our preliminary analysis of a set of old OP plates collected to follow the spectral evolution of a Symbiotic Nova, with special regards to the problem of the spectrophotometric calibration and of the quality of commercial scanners.

  1. Double-layer PVDF transducer and V(z) measurement system for measuring leaky Lamb waves in a piezoelectric plate.

    PubMed

    Lee, Yung-Chun; Kuo, Shi Hoa

    2007-03-01

    This paper presents a new experimental measurement method for leaky Lamb waves propagating in a piezoelectric plate immersed in a conductive fluid. The measurement system is a low-frequency version of lens-less acoustic microscopy which has been developed based on a line-focus double-layer PVDF transducer. The transducer and its defocusing measurement system can perform V(z) measurements on a sample plate immersed in a fluid, and therefore can obtain the leaky Lamb wave velocities with high accuracy. An X-cut LiNbO(3) plate is investigated with this experimental measurement system to find out its fluid-loading effects, especially the conductive loading effects by water of various conductivities. Angular dependence of this conductive loading effect along different propagating directions on the X-cut LiNbO(3) plate is measured. It is found out the conductive loading effects are strongly dependent on the piezoelectric coupling factor. Theoretical calculations based on partial wave theory have also been carried out and compared with experimental data. Good agreements have been observed.

  2. International Space Station Internal Thermal Control System Cold Plate/Fluid-Stability Test: Two Year Update

    NASA Technical Reports Server (NTRS)

    Wieland, Paul; Holt, Mike; Roman, Monsi; Cole, Harold; Daugherty, Steve

    2003-01-01

    Operation of the Internal Thermal Control System (ITCS) Cold Plate/Fluid-Stability Test Facility commenced on September 5, 2000. The facility was intended to provide advance indication of potential problems on board the International Space Station (ISS) and was designed: 1) To be materially similar to the flight ITCS. 2) To allow for monitoring during operation. 3) To run continuously for three years. During the first two years of operation the conditions of the coolant and components were remarkably stable. During this same period of time, the conditions of the ISS ITCS significantly diverged from the desired state. Due to this divergence, the test facility has not been providing information useful for predicting the flight ITCS condition. Results of the first two years are compared with flight conditions over the same time period, showing the similarities and divergences. To address the divergences, the test facility was modified incrementally to more closely match the flight conditions, and to gain insight into the reasons for the divergence. Results of these incremental changes are discussed and provide insight into the development of the conditions on orbit.

  3. CMS Resistive Plate Chamber overview, from the present system to the upgrade phase I

    NASA Astrophysics Data System (ADS)

    Paolucci, P.; Hadjiiska, R.; Litov, L.; Pavlov, B.; Petkov, P.; Dimitrov, A.; Beernaert, K.; Cimmino, A.; Costantini, S.; Guillaume, G.; Lellouch, J.; Marinov, A.; Ocampo, A.; Strobbe, N.; Thyssen, F.; Tytgat, M.; Verwilligen, P.; Yazgan, E.; Zaganidis, N.; Aleksandrov, A.; Genchev, V.; Iaydjiev, P.; Rodozov, M.; Shopova, M.; Sultanov, G.; Ban, Y.; Cai, J.; Xue, Z.; Ge, Y.; Li, Q.; Qian, S.; Avila, C.; Chaparro, L. F.; Gomez, J. P.; Gomez Moreno, B.; Osorio Oliveros, A. F.; Sanabria, J. C.; Assran, Y.; Sharma, A.; Abbrescia, M.; Colaleo, A.; Pugliese, G.; Loddo, F.; Calabria, C.; Maggi, M.; Benussi, L.; Bianco, S.; Colafranceschi, S.; Piccolo, D.; Carrillo, C.; Iorio, O.; Buontempo, S.; Vitulo, P.; Berzano, U.; Gabusi, M.; Kang, M.; Lee, K. S.; Park, S. K.; Shin, S.; Kim, M. S.; Seo, H. K.; Goh, J.; Choi, Y.-I.

    2013-04-01

    Resistive Plate Chambers have been chosen as dedicated trigger muon detector for the Compact Muon Solenoid experiment [1] at the Large Hadron Collider [2] at CERN. The system consists of about 3000 m2 of double gap RPC chambers placed in both the barrel and endcap muon regions. About 5.6 fb-1 (2010-2011) of proton-proton collision data have been used to study the performance of the RPC detector and trigger. A full high voltage scan of all the RPC chambers has been done at beginning of 2011 data taking to evaluate the working point chamber by chamber and to eventually spot aging effects. The excellent behaviour of the RPC detector can be summarized with an average detector efficiency of about 97%, an average cluster size of 1.8 and an intrinsic noise rate of 0.1 Hz/cm2. This is a clear fulfilment of all the requirements decided 18 years ago in the CMS TDR document [3].

  4. Lensless vision system for in-plane positioning of a patterned plate with subpixel resolution.

    PubMed

    Sandoz, Patrick; Jacquot, Maxime

    2011-12-01

    Whereas vision is an efficient way for noncontact sensing of many physical quantities, it assumes a cumbersome imaging system that may be very problematic in confined environments. In such contexts, the design of a compact vision probe can be based on digital holography that is a lensless imaging principle. In this interferometric method, object scenes are reconstructed numerically through wave propagation computations applied to a diffracted optical field recorded as an interferogram. We applied this approach to the visual positioning of a micropatterned glass plate. The pseudoperiodic pattern deposited on the surface is suited for absolute in-plane position determination as well as for fine object-feature interpolation leading to subpixel resolution. Results obtained demonstrate a lateral resolution of 0.1 μm, corresponding to 1/20th of a pixel, from a 150 μm period of the pseudoperiodic pattern and with a demonstrated excursion range of 1.6 cm. In the future, such position encoding could be applied to the backside of standardized sample holders for the easy localization of regions of interest when specimens are transferred from an instrument to another one, for instance in nanotechnology processes.

  5. Automated DNA Sequencing System

    SciTech Connect

    Armstrong, G.A.; Ekkebus, C.P.; Hauser, L.J.; Kress, R.L.; Mural, R.J.

    1999-04-25

    Oak Ridge National Laboratory (ORNL) is developing a core DNA sequencing facility to support biological research endeavors at ORNL and to conduct basic sequencing automation research. This facility is novel because its development is based on existing standard biology laboratory equipment; thus, the development process is of interest to the many small laboratories trying to use automation to control costs and increase throughput. Before automation, biology Laboratory personnel purified DNA, completed cycle sequencing, and prepared 96-well sample plates with commercially available hardware designed specifically for each step in the process. Following purification and thermal cycling, an automated sequencing machine was used for the sequencing. A technician handled all movement of the 96-well sample plates between machines. To automate the process, ORNL is adding a CRS Robotics A- 465 arm, ABI 377 sequencing machine, automated centrifuge, automated refrigerator, and possibly an automated SpeedVac. The entire system will be integrated with one central controller that will direct each machine and the robot. The goal of this system is to completely automate the sequencing procedure from bacterial cell samples through ready-to-be-sequenced DNA and ultimately to completed sequence. The system will be flexible and will accommodate different chemistries than existing automated sequencing lines. The system will be expanded in the future to include colony picking and/or actual sequencing. This discrete event, DNA sequencing system will demonstrate that smaller sequencing labs can achieve cost-effective the laboratory grow.

  6. Comparison study of streptavidin-coated microtitration plates.

    PubMed

    Välimaa, Lasse; Laurikainen, Katja

    2006-01-20

    Streptavidin (SAv)-coated 96-well microtitration plates from commercial suppliers (six plates) and our laboratory (two plates) were tested with respect to their binding capacity for small and large molecules, leaching, well-to-well variation and immunoassay performance. The binding capacities for europium-labelled biotin (Eu-biotin) varied from 4.4 to above 150 pmol/well depending on the plate, and for biotinylated monoclonal antibody from 1.2 to 6.4 pmol/well (190-1030 ng). Incubation for 1 h in regular immunoassay buffer resulted in leaching of 0.6-76 ng of SAv from unwashed wells and 0.5-60 ng from pre-washed wells. The desorbed quantity represented up to 3.4% of the maximal Eu-biotin binding capacity. Coating-related variation of 96 wells (CV% values) ranged from 1.2% to 8.0% when tested with respect to maximum binding capacity. Immunofluorometric assay for TSH as well as enzyme immunoassays for CA125 and PSA revealed immunoassay-related performance of the plates, regarding signal levels, variation and non-specific binding characteristics. Non-specific binding and variation tended to increase in the highest capacity plates, whereas some low capacity plates fulfilled the assay requirements optimally. Good performance with respect to capacity, coating homogeneity or leaching was not by definition reflected in the performance of two-site heterogeneous immunoassays.

  7. Guided growth with a noncannulated screw-plate system for angular deformity of the knee: a preliminary report.

    PubMed

    Lee, Hyun-Joo; Oh, Chang-Wug; Song, Kwang-Soon; Kyung, Hee-Soo; Min, Woo-Kie; Park, Byung-Chul

    2012-07-01

    We assessed the result of guided growth for an angular knee deformity using a 3.5-mm noncannulated screw-plate system. Twenty-seven patients with angular deformities (10 distal femora, 13 proximal tibiae, and four both areas) underwent this procedure with the reconstruction plate and two noncannulated screws. Average age at the time of the procedure was 7.8 years and the average follow-up was 25.7 months. Except for one patient (two knees), 25 of 27 deformities showed a resolved outcome with a neutral alignment. The mechanical lateral distal femoral angle changed an average of 8.3° for 13 months and the medial proximal tibial angle changed an average of 7.7° for 11.8 months in the neutral state. The mean angle between the two screws was -2° in immediate postoperative radiographs and 23° in radiographs taken at the latest follow-up. Two patients showed a rebound outcome and one showed a failure of no correction. There was a superficial infection, but no mechanical failure of the screw and the plate was observed. The noncannulated screw-plate system may play a similar role of guided growth, to correct angular knee deformity, although a large series study with a long follow-up is required for a definitive conclusion.

  8. The European Plate Observing System (EPOS) Services for Solid Earth Science

    NASA Astrophysics Data System (ADS)

    Cocco, Massimo; Atakan, Kuvvet; Pedersen, Helle; Consortium, Epos

    2016-04-01

    The European Plate Observing System (EPOS) aims to create a pan-European infrastructure for solid Earth science to support a safe and sustainable society. The main vision of the European Plate Observing System (EPOS) is to address the three basic challenges in Earth Sciences: (i) unravelling the Earth's deformational processes which are part of the Earth system evolution in time, (ii) understanding the geo-hazards and their implications to society, and (iii) contributing to the safe and sustainable use of geo-resources. The mission of EPOS is to monitor and understand the dynamic and complex Earth system by relying on new e-science opportunities and integrating diverse and advanced Research Infrastructures in Europe for solid Earth Science. EPOS will enable innovative multidisciplinary research for a better understanding of the Earth's physical and chemical processes that control earthquakes, volcanic eruptions, ground instability and tsunami as well as the processes driving tectonics and Earth's surface dynamics. EPOS will improve our ability to better manage the use of the subsurface of the Earth. Through integration of data, models and facilities EPOS will allow the Earth Science community to make a step change in developing new concepts and tools for key answers to scientific and socio-economic questions concerning geo-hazards and geo-resources as well as Earth sciences applications to the environment and to human welfare. EPOS has now started its Implementation Phase (EPOS-IP). One of the main challenges during the implementation phase is the integration of multidisciplinary data into a single e-infrastructure. Multidisciplinary data are organized and governed by the Thematic Core Services (TCS) and are driven by various scientific communities encompassing a wide spectrum of Earth science disciplines. These include Data, Data-products, Services and Software (DDSS), from seismology, near fault observatories, geodetic observations, volcano observations

  9. Development of a System to Measure Austenite Grain Size of Plate Steel Using Laser-Based Ultrasonics

    SciTech Connect

    Lim, C. S.; Hong, S. T.; Yi, J. K.; Choi, S. G.; Oh, K. J.; Nagata, Y.; Yamada, H.; Hamada, N.

    2007-03-21

    A measurement system for austenite grain size of plate steel using laser-based ultrasonics has been developed. At first, the relationship between the ultrasonic attenuation coefficients using longitudinal waves and austenite grain size of samples was investigated in the laboratory experiments. According to the experimental results, the ultrasonic attenuation coefficients showed a good correlation with actual austenite grain sizes. For the next step, the system was installed in a hot rolling pilot plant of plate steel, and it was verified that the austenite grain size could be measured even in the environment of a hot rolling pilot plant. In the experiments, it was also confirmed that the fiber delivery system could deliver Nd:YAG laser beam of 810 mJ/pulse and ultrasonic signals could be obtained successfully.

  10. Biofeedback control analysis using a synchronized system of two CCD video cameras and a force-plate sensor

    NASA Astrophysics Data System (ADS)

    Tsuruoka, Masako; Shibasaki, Ryosuke; Murai, Shunji

    1999-01-01

    The biofeedback control analysis of human movement has become increasingly important in rehabilitation, sports medicine and physical fitness. In this study, a synchronized system was developed for acquiring sequential data of a person's movement. The setup employs a video recorder system linked with two CCD video cameras and fore-plate sensor system, which are configured to stop and start simultaneously. The feedback control movement of postural stability was selected as a subject for analysis. The person's center of body gravity (COG) was calculated by measured 3-D coordinates of major joints using videometry with bundle adjustment and self-calibration. The raw serial data of COG and foot pressure by measured force plate sensor are difficult to analyze directly because of their complex fluctuations. Utilizing auto regressive modeling, the power spectrum and the impulse response of movement factors, enable analysis of their dynamic relations. This new biomedical engineering approach provides efficient information for medical evaluation of a person's stability.

  11. Experimental Fault Diagnosis in Systems Containing Finite Elements of Plate of Kirchoff by Using State Observers Methodology

    NASA Astrophysics Data System (ADS)

    Alegre, D. M.; Koroishi, E. H.; Melo, G. P.

    2015-07-01

    This paper presents a methodology for detection and localization of faults by using state observers. State Observers can rebuild the states not measured or values from points of difficult access in the system. So faults can be detected in these points without the knowledge of its measures, and can be track by the reconstructions of their states. In this paper this methodology will be applied in a system which represents a simplified model of a vehicle. In this model the chassis of the car was represented by a flat plate, which was divided in finite elements of plate (plate of Kirchoff), in addition, was considered the car suspension (springs and dampers). A test rig was built and the developed methodology was used to detect and locate faults on this system. In analyses done, the idea is to use a system with a specific fault, and then use the state observers to locate it, checking on a quantitative variation of the parameter of the system which caused this crash. For the computational simulations the software MATLAB was used.

  12. Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method.

    PubMed

    Gietz, R Daniel; Schiestl, Robert H

    2007-01-01

    Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2-3 h, once the yeast cells have been grown depending on the heat shock used.

  13. Mutual Inductance Problem for a System Consisting of a Current Sheet and a Thin Metal Plate

    NASA Technical Reports Server (NTRS)

    Fulton, J. P.; Wincheski, B.; Nath, S.; Namkung, M.

    1993-01-01

    Rapid inspection of aircraft structures for flaws is of vital importance to the commercial and defense aircraft industry. In particular, inspecting thin aluminum structures for flaws is the focus of a large scale R&D effort in the nondestructive evaluation (NDE) community. Traditional eddy current methods used today are effective, but require long inspection times. New electromagnetic techniques which monitor the normal component of the magnetic field above a sample due to a sheet of current as the excitation, seem to be promising. This paper is an attempt to understand and analyze the magnetic field distribution due to a current sheet above an aluminum test sample. A simple theoretical model, coupled with a two dimensional finite element model (FEM) and experimental data will be presented in the next few sections. A current sheet above a conducting sample generates eddy currents in the material, while a sensor above the current sheet or in between the two plates monitors the normal component of the magnetic field. A rivet or a surface flaw near a rivet in an aircraft aluminum skin will disturb the magnetic field, which is imaged by the sensor. Initial results showed a strong dependence of the flaw induced normal magnetic field strength on the thickness and conductivity of the current-sheet that could not be accounted for by skin depth attenuation alone. It was believed that the eddy current imaging method explained the dependence of the thickness and conductivity of the flaw induced normal magnetic field. Further investigation, suggested the complexity associated with the mutual inductance of the system needed to be studied. The next section gives an analytical model to better understand the phenomenon.

  14. Observation of acoustic streaming in water/sensor plate/thin water layer/128YX-LiNbO3 for realizing disposable digital microfluidic system

    NASA Astrophysics Data System (ADS)

    Kondoh, Jun; Toyoizumi, Hitoshi

    2012-09-01

    One application of a surface acoustic wave (SAW) device is a droplet manipulator. If a sensor is fabricated on the manipulation surface, digital microfluidic system (DMFS) is realized. For disposable application, structure of sensor plate/liquid layer/ 128YX-LiNbO3 is proposed. In this paper, acoustic streaming on a DMFS is experimentally observed. As the streaming in a droplet depends on a contact angle, it in the tank was observed. The radiation patterns on the 128YX-LiNbO3 and sensor plate are differences. The results indicate that the radiation on the sensor plate depends on plate material and thickness.

  15. Renewable liquid reflecting zone plate

    DOEpatents

    Toor, Arthur; Ryutov, Dmitri D.

    2003-12-09

    A renewable liquid reflecting zone plate. Electrodes are operatively connected to a dielectric liquid in a circular or other arrangement to produce a reflecting zone plate. A system for renewing the liquid uses a penetrable substrate.

  16. Preliminary design review package on air flat plate collector for solar heating and cooling system

    NASA Technical Reports Server (NTRS)

    1977-01-01

    Guidelines to be used in the development and fabrication of a prototype air flat plate collector subsystem containing 320 square feet (10-4 ft x 8 ft panels) of collector area are presented. Topics discussed include: (1) verification plan; (2) thermal analysis; (3) safety hazard analysis; (4) drawing list; (5) special handling, installation and maintenance tools; (6) structural analysis; and (7) selected drawings.

  17. Tectonic controls on the stratigraphic architecture and hydrocarbons systems of the Arabian Plate

    SciTech Connect

    Grabowski, G.J. Jr.; Norton, I.O.

    1995-12-31

    Arabian Platform sediments consist of major sequences separated by tectonically controlled unconformities. These tectonic events, at the plate margins, controlled the orientation and distribution of sedimentary facies on the stable platform. Eustacy and subsidence were the principle controls on the actual facies that formed.

  18. 100 Ma: the new frontier for quantitative global models of the coupled brittle-plates/viscous-mantle system.

    NASA Astrophysics Data System (ADS)

    Stotz, Ingo; Iaffaldano, Giampiero; Davies, Rhodri

    2014-05-01

    Over recent decades the body of geophysical datasets has grown substantially and rapidly. Ocean-floor observations now allow one to unravel past plate motions (for instance, in the North Atlantic and Indian Ocean over the past 20 Myr) at the unprecedented temporal resolution of about 1 Myr; and more data is anticipated in the near future. Similarly, our knowledge of continental evolution has grown due to advances in interpreting the records of orogeny and sedimentation. Altogether, these ever-growing datasets allow us to reconstruct the past evolution of Earth's lithospheric plates in greater detail than previously achieved. This is key to unravel the dynamics of geological processes, because reconstructed plate motions and their temporal changes are a powerful probe into the evolving balance of shallow- and deep-rooted forces. Such progress, however, is not yet matched by the ability to quantitatively model past plate-motion changes and, therefore to test hypotheses on the dominant geological controls. The main technical challenge is simulating the rheological behaviour of the plates/mantle system, which varies significantly from viscous to brittle. Classically, computer models for viscous mantle flow and for the piecewise motions of the brittle lithosphere have been developed separately. In recent years, coupling of these two independent classes of models has been pioneered, but only for neo-tectonic scenarios (i.e. past few Myr), and with some limitations as to accounting for the impact of evolving mantle-flow on plate motions. It is now timely to further advance the technical ability to simulate the coupled plates/mantle system through geological time (for instance throughout the Cenozoic and possibly the Cretaceous), and to use the growing body of geophysical data as a primary constraint on these quantitative models. In this project, we take steps in this direction. We build on previous work aimed at coupling two advanced codes for mantle flow and lithosphere

  19. Management of pediatric mandibular fractures using bioresorbable plating system – Efficacy, stability, and clinical outcomes: Our experiences and literature review

    PubMed Central

    Singh, Mahinder; Singh, R.K.; Passi, Deepak; Aggarwal, Mohit; Kaur, Guneet

    2015-01-01

    Aims The purpose of this study was to determine the efficacy and stability of the biodegradable fixation system for treatment of mandible fractures in pediatric patients by measuring the bite force. Methods Sixty pediatric patients with mandibular fractures (36 males, 24 females) were included in this study. The 2.5-mm resorbable plates were adapted along Champy's line of ideal osteosynthesis and secured with four 2.5 mm diameter monocortical resorbable screws, 8 mm in length. All patients were followed for 10 months. Clinical parameters, such as soft tissue infection, nonunion, malunion, implant exposure, malocclusion, nerve injury, and bite force for stability, were prospectively assessed. Results Adequate fixation and primary bone healing was achieved in 100% of the cases. Six minor complications (10%) were observed: 2 soft tissue infections (3%), 1 plate dehiscence (2%), 1 malocclusion (2%), and 2 paresthesia (3%). Conclusion 2.5-mm resorbable plating system along Champy's line of ideal osteosynthesis is a good treatment modality for mandible fractures in pediatric patients. PMID:27195206

  20. Experimental Simulation of Active Control With On-line System Identification on Sound Transmission Through an Elastic Plate

    NASA Technical Reports Server (NTRS)

    1998-01-01

    An adaptive control algorithm with on-line system identification capability has been developed. One of the great advantages of this scheme is that an additional system identification mechanism such as an additional uncorrelated random signal generator as the source of system identification is not required. A time-varying plate-cavity system is used to demonstrate the control performance of this algorithm. The time-varying system consists of a stainless-steel plate which is bolted down on a rigid cavity opening where the cavity depth was changed with respect to time. For a given externally located harmonic sound excitation, the system identification and the control are simultaneously executed to minimize the transmitted sound in the cavity. The control performance of the algorithm is examined for two cases. First, all the water was drained, the external disturbance frequency is swept with 1 Hz/sec. The result shows an excellent frequency tracking capability with cavity internal sound suppression of 40 dB. For the second case, the water level is initially empty and then raised to 3/20 full in 60 seconds while the external sound excitation is fixed with a frequency. Hence, the cavity resonant frequency decreases and passes the external sound excitation frequency. The algorithm shows 40 dB transmitted noise suppression without compromising the system identification tracking capability.

  1. An all-plated, low cost contact system for silicon solar cells

    NASA Technical Reports Server (NTRS)

    Tanner, D. P.; Iles, P. A.; Alexander, P.

    1980-01-01

    The plating sequences Pd-Cr-Cu and Pd-Ni-Cu are demonstrated. The surface was sensitized with a 50 A-thick Pd layer obtained from an immersion bath. After 15 min heating at 400 C in N2, a thin barrier layer of either Cr or Ni was deposited from electroless baths operated at temperatures around 90 C. The sintering process was repeated, and a thin copper layer of 500 A was deposited by electroless means. An electrolytic copper bath was used to build the copper layer to 3-4 micron thicknesses. Cells with good I-V curves were obtained, and the all-plated contacts had good adhesion. Preliminary cost estimates show that the process costs approximately 12 cents per watt excluding the cost of the masking procedure.

  2. Fixation of sternal fracture using absorbable plating system, three years follow-up

    PubMed Central

    Katballe, Niels; Pilegaard, Hans

    2015-01-01

    Sternal fractures occur due to severe chest wall trauma in a small number of patients. They are often conservatively treated. The surgical intervention, although controversial, is indicated in case of deformity, severe pain, and ventilatory complications. We report the first case where absorbable plate has been used to fix a traumatic fracture in a 42-year-old female. After 3 years, the patient is still free of symptoms and CT scanning reveals intact sternal bone structure. PMID:26101659

  3. Drag reducing effects of polymer additives in a plate heat exchanger for the OTEC system

    SciTech Connect

    Kim, N.; Yoon, S.; Kim, C.; Seo, T.

    1999-07-01

    Experiments were undertaken for a 15kW Alfa-Laval plate heat exchanger utilizing polyethylene oxide as a polymer additive. Concentrations of polymer additives were 5, 10, 20, 30, 40, 50, 100, 200 and 400 wppm at 25 C and mass flow rates were 0.6kg/s, 0.7kg/s, 0.8kg/s and 0.9kg/s in normal operating ranges of the plate heat exchanger. The maximum effects of drag reductions were found at 20 wppm polymer concentration and at approximately 0.7kg/s of mass flow rate. The results show that there exist optimum polymer concentration and at approximately 0.7kg/s of mass flow rate. The results show that there exist optimum polymer concentration and mass flow rate for the plate heat exchanger for maximum drag reduction effects. In most cases, drag reduction of approximately 20% has been obtained. It means considerable savings in pumping power for a large size OTEC plant.

  4. Understanding plate-motion changes over the past 100 Myr with quantitative models of the coupled lithosphere/mantle system

    NASA Astrophysics Data System (ADS)

    Stotz, Ingo; Iaffaldano, Giampiero; Rhodri Davies, D.

    2015-04-01

    The volume of geophysical datasets has grown substantially over recent decades. Our knowledge of continental evolution has increased due to advances in interpreting the records of orogeny and sedimentation. Ocean-floor observations now allow one to resolve past plate motions (e.g. in the North Atlantic and Indian Ocean over the past 20 Myr) at temporal resolutions of about 1 Myr. Altogether, these ever-growing datasets allow us to reconstruct the past evolution of Earth's lithospheric plates in greater detail. This is key to unravelling the dynamics of geological processes, because plate motions and their temporal changes are powerful probe into the evolving force balance between shallow- and deep-rooted processes. However, such progress is not yet matched by the ability to quantitatively model past plate-motion changes and, therefore, to test hypotheses on the dominant controls. The main technical challenge is simulating the rheological behaviour of the lithosphere/mantle system, which varies significantly from viscous to brittle. Traditionally computer models for viscous mantle flow on the one hand, and for the motions of the brittle lithosphere on the other hand, have been developed separately. Coupling of these two independent classes of models has been accomplished only for neo-tectonic scenarios, without accounting for the impact of time-evolving mantle-flow (e.g. Iaffaldano and Bunge 2009). However, we have built a coupled model to simulate the lithosphere/mantle system (using SHELLS and TERRA, respectively) through geological time, and to exploit the growing body of geophysical data as a primary constraint on these quantitative models. TERRA is a global spherical finite-element code for mantle convection (e.g. Baumgardner 1985, Bunge et al. 1996, Davies et al. 2013), whilst SHELLS is a thin-sheet finite-element code for lithosphere dynamics (e.g. Bird 1998). Our efforts are focused, in particular, on achieving the technical ability to: (i) simulate the

  5. Weak etalon effect in wave plates can introduce significant FM-to-AM modulations in complex laser systems.

    PubMed

    Dangpeng, Xu; Jianjun, Wang; Mingzhong, Li; Honghuan, Lin; Rui, Zhang; Ying, Deng; Qinghua, Deng; Xiaodong, Huang; Mingzhe, Wang; Lei, Ding; Jun, Tang

    2010-03-29

    The conversion of frequency modulation to amplitude modulation (FM-to-AM) effect is harmful to the high power laser facility based on the phase modulation technique. The FM-to-AM effect of phase modulation pulse induced by the weak etalon effect in wave plates was investigated theoretically and experimentally. A bulk phase modulator with a modulation frequency of 9.2GHz was employed. The numerical simulation results show that the FM-to-AM effect with a temporal modulation depth of 2.5% and 29.7% on the top of the pulse shape was induced by the weak etalon effect in half-wave plate with thickness of 1mm and residual reflectance ratio of 0.5% for 1 pass and 12 passes respectively. On the same condition, the temporal modulation depth is 3.0% and 23.4% respectively in the experiment. The results are in good agreement with numerical simulation results. To our knowledge, it is the first time to introduce the weak etalon effect in wave plates for a complex phase modulation laser system.

  6. Evaluation of the water-treatment ability of silica-doped titanium dioxide-coated glass plates using a cationic coupling reagent based on a flow analytical system.

    PubMed

    Sugita, Tsuyoshi; Mori, Masanobu; Mase, Akinori; Noguchi, Shin-nosuke; Tokutome, Toru; Fujii, Kengo; Hara, Chisato; Katayama, Kenji; Iwamoto, Shinji; Itabashi, Hideyuki

    2015-01-01

    In this study, a photocatalytic plate bound to highly dispersible silica-doped titanium dioxide (SiT) on a trimethoxysilyl-propyldiethylenetriamine (dien)-coated glass plate (dien-plate) was newly synthesized, and was evaluated by a flow analytical (FA) system, which consists of a photocatalytic reactor and a spectrophotometer, to continuously monitor the absorbance of tested chemicals. The method was not required to collect any sample solution at a constant period. The SiT-dien-plate facilitated the photodecomposition of methylene blue (MB) and indigo carmine (InC) in aqueous solutions. Notably, MB was quantitatively photo-decomposed following 18 h of UV-light irradiation, related to the electrostatic adsorption of surface-bound particles. A water-treatment ability of visible-light-responsive vanadium-modified nitrogen/silica co-doped titanium dioxide fixed on the dien-plate was also evaluated by the FA system. It clarified to decompose MB and InC under visible-light irradiation. Finally, the decomposition of a humic substance dissolved from Middle West China peaty soils by the SiT-dien-plate under UV-irradiation was assessed as applying the FA system with a photocatalytic plate.

  7. Building America Case Study: Advanced Extended Plate and Beam Wall System in a Cold-Climate House, Mount Joy, Pennsylvania

    SciTech Connect

    2016-01-01

    This report presents the design and evaluation of a innovative wall system. This highly insulated (high-R) light-frame wall system for use above grade in residential buildings is referred to as Extended Plate & Beam (EP&B). The EP&B design is the first of its kind to be featured in a new construction test house (NCTH) for the DOE Building America program. The EP&B wall design integrates standard building methods and common building products to construct a high-R wall that minimizes transition risks and costs to builders. The EP&B design combines optimized framing with integrated rigid foam sheathing to increase the wall system's R-value and reduce thermal bridging. The foam sheathing is installed between the wall studs and structural wood sheathing. The exterior wood sheathing is attached directly to a framing extension formed by extended top and bottom plates. The exterior wood sheathing can dry to the exterior and provides bracing, a clear drainage plane and flashing surface for window and door openings, and a nailing surface for siding attachment. With support of the DOE Building America program, Home Innovation Research Labs partnered with Lancaster County Career and Technology Center (LCCTC) to build a NCTH in Lancaster, PA to demonstrate the EP&B wall design in a cold climate (IECC climate zone 5A). The results of the study confirmed the benefits of the systems and the viability of its integration into the house construction process.

  8. Isla Guadalupe, a Plate Boundary Observatory Remote GPS System: What's Next in PBO-Mexico?

    NASA Astrophysics Data System (ADS)

    Gonzalez Garcia, J.

    2003-12-01

    As a join project between scientific and technical personnel from Southern California Integrated GPS Network, the University NAVSTAR Consortium, Nanometrics Inc, and CICESE, we installed a VSAT remote communications on Isla Guadalupe in support of data telemetry from a cluster of GPS, meteorological and seismic instrumentation. This Mexican island located between 28\\deg53' and 29\\deg 11'N and 118\\deg 13' to 118\\deg 22'W, lies too far from the main land to allow regular radio link. The station now in operation (GUAX) is near of the early GEOMEX site (GUAD), which recent GPS survey mode result show, is fully located on the Pacific plate within the prediction (1 mm/yr N and 2 mm/yr E) of both the geophysical (NNR-NUVEL1-A) and geodetic (ITRF2000) Plate Tectonic models. Thus, GUAX serve as an important clue to accurately monitor the plate's motion, as well as a reference for studies of California Borderland deformation. During the last 5 years we have built two more sites in northern Baja California: SPMX (1998) and CORX (2000); these together with the IGS station in Ensenada (CICE established in 1995 and replaced by CIC1 in 1999), became part of SCIGN-SOPAC (http://sopac.ucsd.edu, www.scign.org). In Mexico the major organizations working with GPS are INEGI (15 sites) and UNAM (different groups: 20-25 sites). Other State Universities and agencies are increasingly using permanent GPS stations for diverse purposes. It seems that in order to achieve our commitment for PBO-Mexico we must to follow the PGGA/SCIGN/CSRC waybill.

  9. A Module Experimental Process System Development Unit (MEPSDU). [flat plate solar arrays

    NASA Technical Reports Server (NTRS)

    1981-01-01

    The development of a cost effective process sequence that has the potential for the production of flat plate photovoltaic modules which meet the price goal in 1986 of 70 cents or less per Watt peak is described. The major accomplishments include (1) an improved AR coating technique; (2) the use of sand blast back clean-up to reduce clean up costs and to allow much of the Al paste to serve as a back conductor; and (3) the development of wave soldering for use with solar cells. Cells were processed to evaluate different process steps, a cell and minimodule test plan was prepared and data were collected for preliminary Samics cost analysis.

  10. A Curved Image-Plate Detector System for High-Resolution Synchrotron X-ray Diffraction

    SciTech Connect

    Sarin, P.; Haggerty, R; Yoon, W; Knapp, M; Berghaeuser, A; Zschack, P; Karapetrova, E; Yang, N; Kriven, W

    2009-01-01

    The developed curved image plate (CIP) is a one-dimensional detector which simultaneously records high-resolution X-ray diffraction (XRD) patterns over a 38.7 2{theta} range. In addition, an on-site reader enables rapid extraction, transfer and storage of X-ray intensity information in {le}30 s, and further qualifies this detector to study kinetic processes in materials science. The CIP detector can detect and store X-ray intensity information linearly proportional to the incident photon flux over a dynamical range of about five orders of magnitude. The linearity and uniformity of the CIP detector response is not compromised in the unsaturated regions of the image plate, regardless of saturation in another region. The speed of XRD data acquisition together with excellent resolution afforded by the CIP detector is unique and opens up wide possibilities in materials research accessible through X-ray diffraction. This article presents details of the basic features, operation and performance of the CIP detector along with some examples of applications, including high-temperature XRD.

  11. Fenton-biological treatment of reverse osmosis membrane concentrate from a metal plating wastewater recycle system.

    PubMed

    Huang, R M; He, J Y; Zhao, J; Luo, Q; Huang, C M

    2011-04-01

    Although reverse osmosis (RO) has been widely used in the recycling of metal plating wastewater, organic compounds and heavy metals in the RO concentrate are difficult to remove by conventional treatment. A combination process including Fenton oxidation and a biological aerated filter was used to treat RO concentrate containing complex Cu and Ni from metal plating. During the Fenton treatment, Cu and Ni ions were released due to degradation of organic compounds and then removed by pH adjustment and coagulation. The concentrate was further treated using by a biological aerated filter. Optimum conditions were as follows: initial pH of influent of 4.0; dosage of H2O2 of 5.0 mmol l(-1); ratio of n(Fe2+)/n(H2O2) of 0.8; precipitation pH of Cu and Ni ions of 8.0; and a hydraulic retention time of the biological aerated filter of 2.5 h. The results showed that concentrations of effluent COD, Cu and Ni ions were less than 40 mg l(-1), 0.5 mg l(-1) and 0.3 mg l(-1), respectively; this means the treated effluent meets the emission standards for pollutants from electroplating set by China's Environmental Protection Agency.

  12. A wearable force plate system for the continuous measurement of triaxial ground reaction force in biomechanical applications

    NASA Astrophysics Data System (ADS)

    Liu, Tao; Inoue, Yoshio; Shibata, Kyoko

    2010-08-01

    The ambulatory measurement of ground reaction force (GRF) and human motion under free-living conditions is convenient, inexpensive and never restricted to gait analysis in a laboratory environment and is therefore much desired by researchers and clinical doctors in biomedical applications. A wearable force plate system was developed by integrating small triaxial force sensors and three-dimensional (3D) inertial sensors for estimating dynamic triaxial GRF in biomechanical applications. The system, in comparison to existent systems, is characterized by being lightweight, thin and easy-to-wear. A six-axial force sensor (Nitta Co., Japan) was used as a verification measurement device to validate the static accuracy of the developed force plate. To evaluate the precision during dynamic gait measurements, we compared the measurements of the triaxial GRF and the center of pressure (CoP) by using the developed system with the reference measurements made using a stationary force plate and an optical motion analysis system. The root mean square (RMS) differences of the two transverse components (x- and y-axes) and the vertical component (z-axis) of the GRF were 4.3 ± 0.9 N, 6.0 ± 1.3 N and 12.1 ± 1.1 N, respectively, corresponding to 5.1 ± 1.1% and 6.5 ± 1% of the maximum of each transverse component and 1.3 ± 0.2% of the maximum vertical component of GRF. The RMS distance between the two systems' CoP traces was 3.2 ± 0.8 mm, corresponding to 1.2 ± 0.3% of the length of the shoe. Moreover, based on the results of the assessment of the influence of the system on natural gait, we found that gait was almost never affected. Therefore, the wearable system as an alternative device can be a potential solution for measuring CoP and triaxial GRF in non-laboratory environments.

  13. Peen plating

    NASA Technical Reports Server (NTRS)

    Babecki, A. J. (Inventor); Haehner, C. L.

    1973-01-01

    A process for metal plating which comprises spraying a mixture of metallic powder and small peening particles at high velocity against a surface is described. The velocity must be sufficient to impact and bond metallic powder onto the surface. In the case of metal surfaces, the process has as one of its advantages providing mechanical working (hardening) of the surface simultaneously with the metal plating.

  14. European Plate Observing System - the Arctic dimension and the Nordic collaboration

    NASA Astrophysics Data System (ADS)

    Atakan, K.; Heikkinen, P.; Juhlin, C.; Thybo, H.; Vogfjord, K.

    2012-04-01

    Within the framework of the EPOS project, Nordic interests are significant, not only in fundamental scientific issues related to geodynamic processes, but also in terms of the application of these to several central problems such as, hydrocarbon exploration and production including the related environmental issues, CO2 storage (or other toxic waste repositories) in geological formations, geothermal energy (natural and hot-dry rock) utilization and mining, geohazards (earthquakes, landslides and volcanic eruptions) and their consequences to the society. The Arctic dimension including Fennoscandia, the northern North Atlantic and the Arctic Sea constitutes an area of considerable geographical extent within the European plate. The region also contains a significant part of the European plate boundary submerged under the North Atlantic and the Arctic sea, where geodynamic processes such as rifting and fracturing are especially energetic. In particular, where the plate boundary is exposed on land in the South Iceland seismic zone, large earthquakes are frequently observed including two Mw6.5 events in 2000 and one Mw6.3 event in 2008. But, seismic hazard is not confined to the plate boundary. Significant intra-plate earthquakes have recently occurred in the region (Mw6.1 in the continental shelf near Spitsbergen in 2008, Mw5.0 in Southern Sweden in 2008, Mw5.2 near Kaliningrad in 2004) showing that there is considerable seismic hazard in the region. In addition, submarine landslide earthquakes are always of concern due to possible tsunami generation. Volcanic activity occurs on the plate boundary and is particularly strong in the rift zones of Iceland, where on average two volcanic eruptions occur per decade. subaerial volcanic eruptions also occur on Jan Mayen island, farther north on the Mid Atlantic ridge. Together, the Danish seismic network in Greenland, the Norwegian seismic arrays and national network traversing the length of Norway and the Icelandic seismic and

  15. Constraining the upper plate surface deformation across coupled subduction-collision systems

    NASA Astrophysics Data System (ADS)

    Sternai, P.; Jolivet, L.; Menant, A.; Gerya, T.; Avouac, J. P.; Pirouz, M.; Ueda, K.

    2014-12-01

    The relationships between subduction dynamics, continental collision, surface erosion, upper mantle flow and the tectonic history recorded in the continental crust have attracted significant interest. Major results confirmed that mantle convection is a valuable mechanism behind much of the observed dynamic topography and plate motion. However, the degree to which mantle flow controls surface deformation and how this is modulated by erosion are major open questions. Geodetic measurements, coupled with geological observations, enable to achieve a fairly good understanding of the evolution of active features, but the long-term dynamic origins of this kinematics are still controversial. For example, mantle flow is proposed as a driver of plate tectonics, but lithospheric processes such as slab rollback are also known to affect mantle flow. In addition, surface erosion affects crustal deformation, but the interactions between surface mass transfer, crustal strain and deeper dynamics are still widely unknown. More definitive conclusions, especially regarding how the dynamical mantle flow associated to slab tearing and rollback and the mass transfer by surface erosion affect crustal strain, require the investigation of the 3D relationships between the lithosphere and mantle, from the topmost crustal layers to the asthenosphere. We present new high-resolution 3D thermo-mechanical numerical joint models of continental collision, oceanic subduction and slab tearing which allow self-consistent reproduction of first order Tethyan tectonic structures such as back-arc rifting and large scale strike-slip faults accommodating continental escape. We compare these numerical outcomes with observations from the Aegean-Anatolian and eastern Indian-Eurasian margins. We show that the dynamical mantle flow due to slab rollback and tearing can modulate surface deformation by locally enhancing trench retreat and actively dragging the upper plate from below. Further investigations will

  16. Cascades of pi-phase plates: a transparent diffractive focusing system.

    PubMed

    Cagniot, Emmanuel; Fromager, Michael; Godin, Thomas; Traïche, Mohamed; Passilly, Nicolas; Päivänranta, Birgit; Ait-Ameur, Kamel

    2010-07-01

    Typically, refractive lenses are used to focus rays of light, but an alternative way can be found by exploiting diffraction of light. It is well known that cascades of hard-edge apertures are able to focus light but with the great drawback of absorption losses. In this paper, we demonstrate that replacing hard-edge apertures with pi-phase plates within a cascade greatly improves the focusing of collimated Gaussian beams. In addition, we propose a simple model to design this cascade, in particular to find the locations and the radii of the different optics once the focal length has been chosen. This model deduced from numerical simulation is useful for sizing cascades consisting of a high number of components and characterized by a strong focusing ability, without requiring a time-consuming optimization process.

  17. Island-ground single-plate electro-wetting on dielectric device for digital microfluidic systems

    NASA Astrophysics Data System (ADS)

    Cui, Weiwei; Zhang, Menglun; Zhang, Daihua; Pang, Wei; Zhang, Hao

    2014-07-01

    In this paper, we present a single-plate electro-wetting on dielectric (SEWOD) device by integrating an island-ground electrode (IG), which is surrounded by the driving electrodes and looks like an island. Both experiments and theoretical analysis have been conducted to investigate the performance of the IG-SEWOD device. The driving voltage of a fabricated IG-SEWOD has been measured to be 15 V, which is half of that of a floating SEWOD. The digital dynamic properties of the EWOD device are greatly enhanced due to the "double locking" effect and rapid residual charges elimination provided by the IG. The proposed EWOD device shows great potential in constructing advanced microfluidics platforms for bio-chemical detection and disease diagnosis.

  18. A Visualization Method for Corrosion Damage on Aluminum Plates Using an Nd:YAG Pulsed Laser Scanning System

    PubMed Central

    Lee, Inbok; Zhang, Aoqi; Lee, Changgil; Park, Seunghee

    2016-01-01

    This paper proposes a non-contact nondestructive evaluation (NDE) technique that uses laser-induced ultrasonic waves to visualize corrosion damage in aluminum alloy plate structures. The non-contact, pulsed-laser ultrasonic measurement system generates ultrasonic waves using a galvanometer-based Q-switched Nd:YAG laser and measures the ultrasonic waves using a piezoelectric (PZT) sensor. During scanning, a wavefield can be acquired by changing the excitation location of the laser point and measuring waves using the PZT sensor. The corrosion damage can be detected in the wavefield snapshots using the scattering characteristics of the waves that encounter corrosion. The structural damage is visualized by calculating the logarithmic values of the root mean square (RMS), with a weighting parameter to compensate for the attenuation caused by geometrical spreading and dispersion of the waves. An intact specimen is used to conduct a comparison with corrosion at different depths and sizes in other specimens. Both sides of the plate are scanned with the same scanning area to observe the effect of the location where corrosion has formed. The results show that the damage can be successfully visualized for almost all cases using the RMS-based functions, whether it formed on the front or back side. Also, the system is confirmed to have distinguished corroded areas at different depths. PMID:27999252

  19. A Visualization Method for Corrosion Damage on Aluminum Plates Using an Nd:YAG Pulsed Laser Scanning System.

    PubMed

    Lee, Inbok; Zhang, Aoqi; Lee, Changgil; Park, Seunghee

    2016-12-16

    This paper proposes a non-contact nondestructive evaluation (NDE) technique that uses laser-induced ultrasonic waves to visualize corrosion damage in aluminum alloy plate structures. The non-contact, pulsed-laser ultrasonic measurement system generates ultrasonic waves using a galvanometer-based Q-switched Nd:YAG laser and measures the ultrasonic waves using a piezoelectric (PZT) sensor. During scanning, a wavefield can be acquired by changing the excitation location of the laser point and measuring waves using the PZT sensor. The corrosion damage can be detected in the wavefield snapshots using the scattering characteristics of the waves that encounter corrosion. The structural damage is visualized by calculating the logarithmic values of the root mean square (RMS), with a weighting parameter to compensate for the attenuation caused by geometrical spreading and dispersion of the waves. An intact specimen is used to conduct a comparison with corrosion at different depths and sizes in other specimens. Both sides of the plate are scanned with the same scanning area to observe the effect of the location where corrosion has formed. The results show that the damage can be successfully visualized for almost all cases using the RMS-based functions, whether it formed on the front or back side. Also, the system is confirmed to have distinguished corroded areas at different depths.

  20. Analysis and experimental verification of electroacoustic wave energy harvesting in a coupled piezoelectric plate-harvester system

    NASA Astrophysics Data System (ADS)

    Darabi, Amir; Leamy, Michael J.

    2017-03-01

    This paper introduces an analytical framework for predicting wave energy harvested by a circular piezoelectric disk attached to a thin plate. An harmonic point source excitation generates waves that are then incident on a piezoelectric disk—summing responses due to all such excitation enables general forcing profiles to be considered. The analysis approach decomposes the coupled system into two subdomains, one being the piezoelectric disk, and the other an infinite plate for which a Green's function is readily available. Interaction forces between the two subdomains couple the problems and lead to a closed-form solution for the propagation, transmission, and reflection of waves over the entire domain. In addition, the voltage generated by the harvester is calculated using coupled electromechanical equations. The analysis approach is first validated by comparing predicted response quantities to those computed using numerical simulations, documenting good agreement. The system is then studied in the frequency domain and the optimum harvester resistance is found for generating the most electrical power. Representative experiments are carried out to demonstrate the validity of the analytical approach and verify the harvested power versus resistance trend.

  1. Matrix based system of isotretinoin as nail lacquer to enhance transungal delivery across human nail plate.

    PubMed

    Joshi, Monika; Sharma, Vijay; Pathak, Kamla

    2015-01-15

    The project was aimed at development of isotretinoin nail lacquer and assessment of its penetration efficiency across human nail plate. Preliminary studies (hydration enhancement factor and SEM) aided the selection of thioglycolic acid as permeation and eugenol was selected as local anesthetic in the formulation. The nail lacquer was optimized by 3(2) factorial design and a total of nine formulations were prepared and screened. In vitro adhesion and ex vivo permeation (cumulative drug permeation per unit area (CDP/A) = 6.61 ± 0.57 mg/cm(2)) across bovine hoof guided the selection of F3 as optimized formulation that was improvised. Viscosity adjustments to improve handling characteristics were affected by incorporation of ethyl cellulose (6%; F3M1) that scaled the viscosity to 312.681 cp and insignificantly (p > 0.05) affected CDP/A (6.32 ± 0.45 mg/cm(2)). In comparison to marketed preparation (Retino-A cream) F3M1 afforded two fold increase in CDP/A. The permeation characteristics were defined by Higuchi model (r(2) = 0.964) and flux value of 176 μg/cm(2)/h. Confocal laser scanning microscopy, after 72 h of nail lacquer application, revealed extensive distribution of the fluorescent tracer across the human nail plate in comparison to control that was confined to the top layer. Conclusively, an efficacious and stable nail lacquer of isotretinoin was developed for potential clinical topical use to target the drug to nail bed in treatment of nail psoriasis.

  2. Ionospheric plasma flow about a system of electrically biased flat plates. M.S. Thesis - Cleveland State Univ. Final Report

    NASA Technical Reports Server (NTRS)

    Herr, Joel L.

    1993-01-01

    The steady state interaction of two electrically biased parallel plates immersed in a flowing plasma characteristic of low earth orbit is studied numerically. Fluid equations are developed to describe the motion of the cold positively charged plasma ions, and are solved using finite-differences in two dimensions on a Cartesian grid. The behavior of the plasma electrons is assumed to be described by the Maxwell-Boltzmann distribution. Results are compared to an analytical and a particle simulation technique for a simplified flow geometry consisting of a single semi-infinite negatively biased plate. Comparison of the extent of the electrical disturbance into the flowing plasma and the magnitude of the current collected by the plate is very good. The interaction of two equally biased parallel plates is studied as a function of applied potential. The separation distance at which the current collected by either plate decreases by five and twenty percent is determined as a function of applied potential. The percent decreases were based on a non-interacting case. The decrease in overall current is caused by a decrease in ionic density in the region between the plates. As the separation between the plates decreases, the plates collect the ions at a faster rate than they are supplied to the middle region by the oncoming plasma flow. The docking of spacecraft in orbit is simulated by moving two plates of unequal potential toward one another in a quasi-static manner. One plate is held at a large negative potential while the other floats electrically in the resulting potential field. It is found that the floating plate does not charge continuously negative as it approaches the other more negatively biased plate. Instead, it charges more and then less negative as ionic current decreases and then increases respectively upon approach. When the two plates come into contact, it is expected that the electrically floating plate will charge rapidly negative to a potential near that of

  3. Parallel electromembrane extraction in the 96-well format.

    PubMed

    Eibak, Lars Erik Eng; Rasmussen, Knut Einar; Oiestad, Elisabeth Leere; Pedersen-Bjergaard, Stig; Gjelstad, Astrid

    2014-05-30

    The repeatability and extraction recoveries of parallel electromembrane extraction (Pa-EME) was thoroughly investigated in the present project. Amitriptyline, fluoxetine, and haloperidol were isolated from eight samples of pure water, undiluted human plasma, and undiluted human urine, respectively; in total 24 samples were processed in parallel. The repeatability was found to be independent of the different sample matrices (pure water samples, human plasma, and water) processed in parallel, although the respective samples contained different matrix components. In another experiment seven of the 24 wells were perforated. Even though the perforation caused the total current level in the Pa-EME setup to increase, the intact circuits were unaffected by the collapse in seven of the circuits. In another approach, exhaustive extraction of amitriptyline, fluoxetine, and haloperidol was demonstrated from pure water samples. Amitriptyline and haloperidol were also isolated exhaustively from undiluted human plasma samples; the extraction recovery of fluoxetine from undiluted human plasma was 81%. Finally, the sample throughput was increased with the Pa-EME configuration. The extraction recoveries were investigated by processing 1, 8, 68, or 96 samples in parallel in 10min; neither the extraction recoveries nor the repeatability was affected by the total numbers of samples. Eventually, the Pa-EME was combined with ultra performance liquid chromatography (UPLC) to combine high-throughput sample preparation with high-throughput analytical instrumentation. The aim of the present investigation was to demonstrate the potential of electromembrane extraction as a high throughput sample preparation platform; and hopefully to increase the interest for EME in the bioanalytical field to solve exisiting and novel analytical challenges.

  4. Fully back-end TSV process by Cu electro-less plating for 3D smart sensor systems

    NASA Astrophysics Data System (ADS)

    Santagata, F.; Farriciello, C.; Fiorentino, G.; van Zeijl, H. W.; Silvestri, C.; Zhang, G. Q.; Sarro, P. M.

    2013-05-01

    A fully back-end process for high-aspect ratio through-silicon vias (TSVs) for 3D smart sensor systems is developed. Atomic layer deposition of TiN provides a highly conformal barrier as well as a seed layer for metal plating. Cu electro-less plating on the chemically activated TiN surfaces is applied to uniformly fill the TSVs in a significantly shorter time (2 h for 300 μm deep and 20 μm wide TSVs) than with Cu bottom-up electroplating (>20 h). The process is CMOS compatible and can be performed after the last metalization step, making it a fully back-end process (VIA-last approach). Wafers containing metal interconnections on both sides are in fact used as demonstrator. Four-terminal 3D Kelvin structures are fabricated and characterized. An average resistance value of 650 mΩ is measured for 300 μm deep TSVs with an aspect ratio of 15. The crosstalk between adjacent TSVs is also measured by means of S-parameters characterization on dedicated RF test structures. The closest TSVs (75 μm) show a reciprocal crosstalk of less than -20 dB at 30 GHz.

  5. In vitro maturation system for individual culture of bovine oocytes using micro-volume multi-well plate.

    PubMed

    Nagano, Masashi; Kang, Sung-Sik; Koyama, Keisuke; Huang, Weiping; Yanagawa, Yojiro; Takahashi, Yoshiyuki

    2013-11-01

    As a preliminary study for the development of individual in vitro maturation (IVM) culture of bovine oocytes, a multi-well (MW) plate was used. Maturation, fertilization and development to blastocysts were examined and compared with those of IVM oocytes cultured in 50-microl droplets in groups and in 10-microl droplets individually. The maturation rates were similar in all experimental groups. Normal fertilization rates in MW and 50-microl droplets were similar, but lower in 10-microl droplets (p < 0.01). The blastocyst rate in 10-microl droplets tended to be lower than those in MW (p = 0.15) and 50-microl droplets (p = 0.19). These results indicate that an IVM system using MW supports the acquisition of developmental competence by bovine oocytes the same as conventional group IVM culture.

  6. Numerical analysis on performance of naphthalene phase change thermal storage system in aluminum plate-fin unit

    NASA Astrophysics Data System (ADS)

    Yang, Lin; Peng, Hao; Ling, Xiang; Dong, Huihua

    2015-02-01

    This paper presents a numerical simulation for the prediction of time, temperature and liquid fraction during the melting and solidification process in the latent heat storage of phase changed materials (PCM). The mathematical model, regarding the solid-liquid phase change heat transfer based on the enthalpy formulation, has been proposed. Naphthalene was used as the PCM which is contained in up layer of plate-fin thermal storage. Transient simulations were performed using a commercial computational fluid dynamics package, Fluent, based on the finite volume method. This computational model endeavored to describe both the melting and solidification processes of the PCM. A series of numerical calculations have been done in order to analyses the influence of several heat transfer fluid operating conditions and several fin geometric parameters on the thermal storage unit. Numerical results, which could be used for operating conditions and geometry optimization, provide guideline for the design of the latent thermal energy storage system.

  7. Performance advantages of two-axis tracking for large flat-plate photovoltaic energy systems

    SciTech Connect

    Gay, C.F.; Wilson, J.H.; Yerkes, J.W.

    1982-09-01

    Daily and annual energy-delivery performance is compared for large-scale fixed-array and two-axis tracking photovoltaic generating systems similar to the ASI Project at Lugo, California. Systems of equal peak-watt rating and systems of different sizes but equal annual energy output are compared in the Lugo setting. Site sensitivities are discussed. For the site studied, it is observed that a fixed-array system would use about 40% more modules than a two-axis tracking system, for equal annual energy.

  8. Performance advantages of two-axis tracking for large flat-plate photovoltaic energy systems

    NASA Astrophysics Data System (ADS)

    Gay, C. F.; Yerkes, J. W.; Wilson, J. H.

    Daily and annual energy-delivery performance is compared for large-scale fixed-array and two-axis tracking photovoltaic generating systems similar to the ASI Project at Lugo, CA. Systems of equal peak-watt rating and systems of different sizes but equal annual energy output are compared in the Lugo setting. Site sensitivities are discussed. For the site studied, it is observed that a fixed-array system would use about 40 percent more modules than a two-axis tracking system, for equal annual energy.

  9. Upper plate deformation and seismic barrier in front of Nazca subduction zone: The Chololo Fault System and active tectonics along the Coastal Cordillera, southern Peru

    NASA Astrophysics Data System (ADS)

    Audin, Laurence; Lacan, Pierre; Tavera, Hernando; Bondoux, Francis

    2008-11-01

    The South America plate boundary is one of the most active subduction zone. The recent Mw = 8.4 Arequipa 2001 earthquake ruptured the subduction plane toward the south over 400 km and stopped abruptly on the Ilo Peninsula. In this exact region, the subduction seismic crisis induced the reactivation of continental fault systems in the coastal area. We studied the main reactivated fault system that trends perpendicular to the trench by detailed mapping of fault related-geomorphic features. Also, at a longer time scale, a recurrent Quaternary transtensive tectonic activity of the CFS is expressed by offset river gullies and alluvial fans. The presence of such extensional fault systems trending orthogonal to the trench along the Coastal Cordillera in southern Peru is interpreted to reflect a strong coupling between the two plates. In this particular case, stress transfer to the upper plate, at least along the coastal fringe, appears to have induced crustal seismic events that were initiated mainly during and after the 2001 earthquake. The seafloor roughness of the subducting plate is usually thought to be a cause of segmentation along subduction zones. However, after comparing and discussing the role of inherited structures within the upper plate to the subduction zone segmentation in southern Peru, we suggest that the continental structure itself may exert some feedback control on the segmentation of the subduction zone and thus participate to define the rupture pattern of major subduction earthquakes along the southern Peru continental margin.

  10. Effect of different culture systems and 3, 5, 3'-triiodothyronine/follicle-stimulating hormone on preantral follicle development in mice.

    PubMed

    Zhang, Cheng; Wang, Xiaoxia; Wang, Zhengpin; Niu, Wanbao; Zhu, Baochang; Xia, Guoliang

    2013-01-01

    The mechanical method to isolate preantral follicle has been reported for many years. However, the culture systems in vitro are still unstable. The aim of this study was to analyze the effect of the culture system of mice preantral follicles on the follicular development in vitro. The results showed that the 96-well plate system was the most effective method for mice follicle development in vitro (volume change: 51.71%; survival rate: 89%, at day 4). Follicle-stimulating hormone (FSH) and Thyroid hormone (TH) are important for normal follicular development and dysregulation of hormones are related with impaired follicular development. To determine the effect of hormone on preantral follicular development, we cultured follicle with hormones in the 96-well plate culture system and found that FSH significantly increased preantral follicular growth on day 4. The FSH-induced growth action was markedly enhanced by T₃ although T₃ was ineffective alone. We also demonstrated by QRT-PCR that T₃ significantly enhanced FSH-induced up-regulation of Xiap mRNA level. Meanwhile, Bad, cell death inducer, was markedly down-regulated by the combination of hormones. Moreover, QRT-PCR results were also consistent with protein regulation which detected by Western Blotting analysis. Taken together, the findings of the present study demonstrate that 96-well plate system is an effective method for preantral follicle development in vitro. Moreover, these results provide insights on the role of thyroid hormone in increasing FSH-induced preantral follicular development, which mediated by up-regulating Xiap and down-regulating Bad.

  11. A new method for analytical solution of inplane free vibration of rectangular orthotropic plates based on the analysis of infinite systems

    NASA Astrophysics Data System (ADS)

    Papkov, S. O.

    2016-05-01

    In this paper, a new method for in-plane free vibration analysis of rectangular orthotropic plate is presented. The cases for completely clamped and completely free plates are considered in detail. The boundary value problem is essentially reduced to infinite systems of linear algebraic equations. New mathematical theory and associated results are presented for investigating the asymptotic behavior of non-trivial solution of quasi-regular infinite system. By enhancing the theory, an effective and accurate algorithm for determining the natural frequencies and modes shapes vibrations is developed. The accuracy and computational efficiency of the method are demonstrated by examples.

  12. Anatomy of an extinct magmatic system along a divergent plate boundary: Alftafjordur, Iceland

    NASA Astrophysics Data System (ADS)

    Urbani, S.; Trippanera, D.; Porreca, M.; Kissel, C.; Acocella, V.

    2015-08-01

    Recent rifting episodes highlight the role of magmatic systems with propagating dikes on crustal spreading. However, our knowledge of magmatic systems is usually limited to surface observations and geophysical data. Eastern Iceland allows direct access to extinct and eroded deeper magmatic systems. Here we collected field structural and AMS (anisotropy of magnetic susceptibility) data on 187 and 19 dikes, respectively, in the 10-12 Ma old Alftafjordur magmatic system. At a paleodepth of ~1.5 km, the extension due to diking is at least 1-2 orders of magnitude larger than that induced by regional tectonics, confirming magmatism as the key mechanism for crustal spreading. This magma-induced extension, inferred from the aspect ratio of the magmatic system, was of ~8 mm/yr, lower than the present one. AMS data suggest that most of dikes have geometrically normal fabric, at least at the margins, consistent with prevalent subvertical magma flow and propagation.

  13. Thermal and optical performance of encapsulation systems for flat-plate photovoltaic modules

    NASA Technical Reports Server (NTRS)

    Minning, C. P.; Coakley, J. F.; Perrygo, C. M.; Garcia, A., III; Cuddihy, E. F.

    1981-01-01

    The electrical power output from a photovoltaic module is strongly influenced by the thermal and optical characteristics of the module encapsulation system. Described are the methodology and computer model for performing fast and accurate thermal and optical evaluations of different encapsulation systems. The computer model is used to evaluate cell temperature, solar energy transmittance through the encapsulation system, and electric power output for operation in a terrestrial environment. Extensive results are presented for both superstrate-module and substrate-module design schemes which include different types of silicon cell materials, pottants, and antireflection coatings.

  14. Use of a Slick-Plate as a Contingency Exercise Surface for the Treadmill With Vibration Isolation System

    NASA Technical Reports Server (NTRS)

    Loehr, James A.; Lee, Stuart M. C.; Schneider, Suzanne M.

    2003-01-01

    The treadmill with vibration isolation system (TVIS) was developed to counteract cardiovascular, musculoskeletal, and neurovestibular deconditioning during long-duration missions to the International Space Station (ISS). However, recent hardware failures have necessitated the development of a short-term, temporary contingency exercise countermeasure for TVIS until nominal operations could be restored. The purpose of our evaluation was twofold: 1) to examine whether a slick-plate/contingency exercise surface (CES) could be used as a walking/running surface and could elicit a heart rate (HR) greater than or equal to 70% HR maximum and 2) to determine the optimal hardware configuration, in microgravity, to simulate running/walking in a 1-g environment. One subject (male) participated in the slick surface evaluation and two subjects (one male, one female) participated in the microgravity evaluation of the slick surface configuration. During the slick surface evaluation, the subject was suspended in a parachute harness and bungee cord configuration to offset the subject#s body weight. Using another bungee cord configuration, we added a vertical load back to the subject, who was then asked to run for 20 minutes on the slick surface. The microgravity evaluation simulated the ISS TVIS, and we evaluated two different slick surfaces (Teflon surface and an aluminum surface coated with Tufram) for use as a CES. We evaluated each surface with the subject walking and running, with and without a handrail, and while wearing either socks or nylon booties over shoes. In the slick surface evaluation, the subject ran for 20 minutes and reached a maximum HR of 170 bpm. In the microgravity evaluation, the subjects chose the aluminum plate coated with Tufram as the CES, while wearing a pair of nylon booties over running shoes and using a handrail, as the optimal hardware configuration.

  15. Plate electronics

    NASA Astrophysics Data System (ADS)

    Carlowicz, Michael

    Using a Cray T3D supercomputer and a simple assumption about the physical character of Earth's mantle, a pair of researchers from the University of California at Berkeley have built a computer model that may help explain why the planet's tectonic plates look the way they do.In creating a three-dimensional numerical simulation of convection in the Earth's interior, UC researchers Hans-Peter Bunge and Mark Richards simplified their model to account for just one major physical effect: that the viscosity of the mantle increases with depth. Reviewing some recent—but not yet widely accepted—seismic data, Bunge and Richards assumed for the sake of the model that the viscosity of the mantle increases by a factor of 30 from the lithosphere to the core-mantle boundary. Relying on that assumption, the pair ran the model for nearly three weeks on a supercomputer at Los Alamos National Laboratory and found that the simulation produced an effect similar to what we see on the surface of Earth. The model produced a surface paralleling the actual width of plates and the geometry of the plate boundaries.

  16. Structural-acoustic model of a rectangular plate-cavity system with an attached distributed mass and internal sound source: Theory and experiment

    NASA Astrophysics Data System (ADS)

    Pirnat, Miha; Čepon, Gregor; Boltežar, Miha

    2014-03-01

    In this paper three approaches are combined to develop a structural-acoustic model of a rectangular plate-cavity system with an attached distributed mass and internal sound source. The first approach results from a recently presented analysis based on the Rayleigh-Ritz method and is used to circumvent the difficulties in obtaining the natural frequencies and mode shapes of a plate with an attached, distributed mass. Furthermore, different plate boundary conditions can be accommodated. The resulting mode shapes are defined as continuous functions; this is advantageous as they can be directly used in the second approach, i.e., the classic modal-interaction approach in order to obtain the coupled equations of the system. Finally, in the third approach a group of point sources emitting a pressure pulse in the time domain is used to model an internal sound source. For the validation of the developed model an experiment was conducted in two configurations using a simply supported aluminium plate and a clamped plate coupled with a plexiglas box containing a loudspeaker. Good agreement was found between the analytical and experimental data.

  17. A high-capacity LC/MS system for the bioanalysis of samples generated from plate-based metabolic screening.

    PubMed

    Janiszewski, J S; Rogers, K J; Whalen, K M; Cole, M J; Liston, T E; Duchoslav, E; Fouda, H G

    2001-04-01

    HPLC/MS is a linear technique characterized by serial injection and analysis of individual samples. Parallel-format high-throughput screens for druglike properties present a significant analytical challenge. Analysis speed and system ruggedness are key requirements for bioanalysis of thousands of samples per day. The tasks involved in LC/MS analysis are readily divided into three areas, sample preparation/liquid handling, LC/MS method building/sample analysis, and data processing. Several automation and multitasking strategies were developed and implemented to minimize plating and liquid handling errors, reduce dead times within the analysis cycle, and allow for comprehensive review of data. Delivering multiple samples to multiple injectors allows the autosampler time to complete its wash cycles and aspirate the next set of samples while the previous set is being analyzed. A dual-column chromatography system provides column cycling and peak stacking and allows rapid throughput using conventional LC equipment. Collecting all data for a compound into a single file greatly reduces the number of data files collected, increases the speed of data collection, allows rugged and complete review of all data, and provides facile data management. The described systems have analyzed over 40 000 samples per month for two years and have the capacity for over 2000 samples per instrument per day.

  18. Real-time ArcGIS and heterotrophic plate count based chloramine disinfectant control in water distribution system.

    PubMed

    Bai, Xiaohui; Zhi, Xinghua; Zhu, Huifeng; Meng, Mingqun; Zhang, Mingde

    2015-01-01

    This study investigates the effect of chloramine residual on bacteria growth and regrowth and the relationship between heterotrophic plate counts (HPCs) and the concentration of chloramine residual in the Shanghai drinking water distribution system (DWDS). In this study, models to control HPCs in the water distribution system and consumer taps are also developed. Real-time ArcGIS was applied to show the distribution and changed results of the chloramine residual concentration in the pipe system by using these models. Residual regression analysis was used to get a reasonable range of the threshold values that allows the chloramine residual to efficiently inhibit bacteria growth in the Shanghai DWDS; the threshold values should be between 0.45 and 0.5 mg/L in pipe water and 0.2 and 0.25 mg/L in tap water. The low residual chloramine value (0.05 mg/L) of the Chinese drinking water quality standard may pose a potential health risk for microorganisms that should be improved. Disinfection by-products (DBPs) were detected, but no health risk was identified.

  19. Flexible Architecture System & Topology License Plate Recognition (Fast LPR) and Concept of Operations in Thailand

    DTIC Science & Technology

    2008-09-01

    Topology: System layout capability to support image capture remotely (distributed) from the GUI operation, localized “ toll booth ” type deployments...Scenarios .....................................................................119 a. Gate / Toll Post Fixed Scenario...111 Figure 34. Typical FAST LPR Gate Configuration ........................................................121 Figure 35. Toll

  20. Tests and Evaluation of Upgraded Flat-Plate and Waffle-Slab Floor Systems

    DTIC Science & Technology

    1983-12-01

    slab floor center portion. Therefore, in the case of Crisis Relocation Planning implementation involving the upgrading of floor systems, allowances...L. Huff managed the project. Mr. M. K. McVay planned and supervised the experiments and Mr. S. C. Woodson analyzed the test results and prepared this...Relocation Planning (CRP). CRP would call for the following actions by FEMA during a time of developing interna- tional crisis when a nuclear war is imminent

  1. Cadmium plating replacements

    SciTech Connect

    Nelson, M.J.; Groshart, E.C.

    1995-03-01

    The Boeing Company has been searching for replacements to cadmium plate. Two alloy plating systems seem close to meeting the needs of a cadmium replacement. The two alloys, zinc-nickel and tin-zinc are from alloy plating baths; both baths are neutral pH. The alloys meet the requirements for salt fog corrosion resistance, and both alloys excel as a paint base. Currently, tests are being performed on standard fasteners to compare zinc-nickel and tin-zinc on threaded hardware where cadmium is heavily used. The Hydrogen embrittlement propensity of the zinc-nickel bath has been tested, and just beginning for the tin-zinc bath. Another area of interest is the electrical properties on aluminum for tin-zinc and will be discussed. The zinc-nickel alloy plating bath is in production in Boeing Commercial Airplane Group for non-critical low strength steels. The outlook is promising that these two coatings will help The Boeing Company significantly reduce its dependence on cadmium plating.

  2. Cadmium plating replacements

    NASA Technical Reports Server (NTRS)

    Nelson, Mary J.; Groshart, Earl C.

    1995-01-01

    The Boeing Company has been searching for replacements to cadmium plate. Two alloy plating systems seem close to meeting the needs of a cadmium replacement. The two alloys, zinc-nickel and tin-zinc are from alloy plating baths; both baths are neutral pH. The alloys meet the requirements for salt fog corrosion resistance, and both alloys excel as a paint base. Currently, tests are being performed on standard fasteners to compare zinc-nickel and tin-zinc on threaded hardware where cadmium is heavily used. The Hydrogen embrittlement propensity of the zinc-nickel bath has been tested, and just beginning for the tin-zinc bath. Another area of interest is the electrical properties on aluminum for tin-zinc and will be discussed. The zinc-nickel alloy plating bath is in production in Boeing Commercial Airplane Group for non-critical low strength steels. The outlook is promising that these two coatings will help The Boeing Company significantly reduce its dependence on cadmium plating.

  3. Analysis of a high-throughput cone-and-plate apparatus for the application of defined spatiotemporal flow to cultured cells.

    PubMed

    Spruell, Christopher; Baker, Aaron B

    2013-06-01

    The shear stresses derived from blood flow regulate many aspects of vascular and immunobiology. In vitro studies on the shear stress-mediated mechanobiology of endothelial cells have been carried out using systems analogous to the cone-and-plate viscometer in which a rotating, low-angle cone applies fluid shear stress to cells grown on an underlying, flat culture surface. We recently developed a device that could perform high-throughput studies on shear-mediated mechanobiology through the rotation of cone-tipped shafts in a standard 96-well culture plate. Here, we present a model of the three-dimensional flow within the culture wells with a rotating, cone-tipped shaft. Using this model we examined the effects of modifying the design parameters of the system to allow the device to create a variety of flow profiles. We first examined the case of steady-state flow with the shaft rotating at constant angular velocity. By varying the angular velocity and distance of the cone from the underlying plate we were able to create flow profiles with controlled shear stress gradients in the radial direction within the plate. These findings indicate that both linear and non-linear spatial distributions in shear stress can be created across the bottom of the culture plate. In the transition and "parallel shaft" regions of the system, the angular velocities needed to provide high levels of physiological shear stress (5 Pa) created intermediate Reynolds number Taylor-Couette flow. In some cases, this led to the development of a flow regime in which stable helical vortices were created within the well. We also examined the system under oscillatory and pulsatile motion of the shaft and demonstrated minimal time lag between the rotation of the cone and the shear stress on the cell culture surface.

  4. Free convective heat transfer with hall effects, heat absorption and chemical reaction over an accelerated moving plate in a rotating system

    NASA Astrophysics Data System (ADS)

    Hussain, S. M.; Jain, J.; Seth, G. S.; Rashidi, M. M.

    2017-01-01

    The unsteady MHD free convective heat and mass transfer flow of an electrically conducting, viscous and incompressible fluid over an accelerated moving vertical plate in the presence of heat absorption and chemical reaction with ramped temperature and ramped surface concentration through a porous medium in a rotating system is studied, taking Hall effects into account. The governing equations are solved analytically with the help of Laplace transform technique. The unified closed-form expressions are obtained for fluid velocity, fluid temperature, species concentration, skin friction, Nusselt number and Sherwood numbers. The effects of various parameters on fluid velocity, fluid temperature and species concentration are discussed by graphs whereas numerical values of skin friction, Nusselt and Sherwood numbers are presented in tabular form for different values of pertinent flow parameters. The numerical results are also compared with free convective flow near ramped temperature plate with ramped surface concentration with the corresponding flow near isothermal plate with uniform surface concentration.

  5. Metal vapor arc ion plating

    DOEpatents

    Bertram, L.A.; Fisher, R.W.; Mattox, D.M.; Zanner, F.J.

    1986-09-09

    A method and apparatus for ion plating are described. The apparatus uses more negative than a first electrode voltage in a vacuum arc remelt system to attract low energy ions from the anode electrode to the article to be plated. 2 figs.

  6. Micro-channel plate detector

    DOEpatents

    Elam, Jeffrey W.; Lee, Seon W.; Wang, Hsien -Hau; Pellin, Michael J.; Byrum, Karen; Frisch, Henry J.

    2015-09-22

    A method and system for providing a micro-channel plate detector. An anodized aluminum oxide membrane is provided and includes a plurality of nanopores which have an Al coating and a thin layer of an emissive oxide material responsive to incident radiation, thereby providing a plurality of radiation sensitive channels for the micro-channel plate detector.

  7. Ridge subduction sparked reorganization of the Pacific plate-mantle system 60-50 million years ago

    NASA Astrophysics Data System (ADS)

    Seton, Maria; Flament, Nicolas; Whittaker, Joanne; Müller, R. Dietmar; Gurnis, Michael; Bower, Dan J.

    2015-03-01

    A reorganization centered on the Pacific plate occurred ~53-47 million years ago. A "top-down" plate tectonic mechanism, complete subduction of the Izanagi plate, as opposed to a "bottom-up" mantle flow mechanism, has been proposed as the main driver. Verification based on marine geophysical observations is impossible as most ocean crust recording this event has been subducted. Using a forward modeling approach, which assimilates surface plate velocities and shallow thermal structure of slabs into mantle flow models, we show that complete Izanagi plate subduction and margin-wide slab detachment induced a major change in sub-Pacific mantle flow, from dominantly southward before 60 Ma to north-northeastward after 50 Ma. Our results agree with onshore geology, mantle tomography, and the inferred motion of the Hawaiian hot spot and are consistent with a plate tectonic process driving the rapid plate-mantle reorganization in the Pacific hemisphere between 60 and 50 Ma. This reorganization is reflected in tectonic changes in the Pacific and surrounding ocean basins.

  8. Seismic Anisotropy and Mantle Flow from Ridge to Trench Below the Gorda-Juan de Fuca Plate System

    NASA Astrophysics Data System (ADS)

    Martin-Short, R.; Allen, R. M.; Bastow, I. D.; Richards, M. A.; Totten, E. J.

    2015-12-01

    Tectonic plates are underlain by a low viscosity layer of the mantle, the asthenosphere, which flows. Flow in the asthenosphere may be induced by motion of the overriding plate, or by deeper mantle convection. Measurement of seismic anisotropy, the directional dependence of seismic wave speed, is an important tool in understanding mantle structure and dynamics, and is often used to infer information about asthenospheric flow geometry. However, isolation of asthenospheric signals is challenging because most seismometers are located on the continents, whose complex structure influences seismic waves en-route to the surface. Thus the challenge is to record seismic data on oceanic lithosphere, which is much thinner and simpler. We present stacked shear wave splitting results from phases 1 to 3 of the Cascadia Initiative: An ambitious, large-scale deployment of offshore seismometers across the Gorda and Juan-de-Fuca plates. Fast splitting directions (FSD) can approximate mantle flow geometry, thus for the first time allowing an interpretation of flow beneath an ocean basin from ridge to trench. The Juan-de-Fuca plate appears able to influence mantle flow: FSD rotate towards the absolute plate motion direction (APM) with increasing distance from the ridge. In contrast, Gorda FSD align with the motion of the adjacent Pacific plate rather than Gorda APM. These observations suggest that drag caused by motion the Pacific plate controls asthenospheric flow beneath Gorda, and thus that the Gorda plate may be decoupled from the asthenosphere. We also construct a simple geodynamic model of this situation, which supports its plausibility. Our findings imply that tectonic plates must reach a minimum size and speed before they are able to exert influence on asthenospheric flow.

  9. Two-dimensional laser servoing for precision motion control of an ODV robotic license plate recognition system

    NASA Astrophysics Data System (ADS)

    Song, Zhen; Moore, Kevin L.; Chen, YangQuan; Bahl, Vikas

    2003-09-01

    As an outgrowth of series of projects focused on mobility of unmanned ground vehicles (UGV), an omni-directional (ODV), multi-robot, autonomous mobile parking security system has been developed. The system has two types of robots: the low-profile Omni-Directional Inspection System (ODIS), which can be used for under-vehicle inspections, and the mid-sized T4 robot, which serves as a ``marsupial mothership'' for the ODIS vehicles and performs coarse resolution inspection. A key task for the T4 robot is license plate recognition (LPR). For a successful LPR task without compromising the recognition rate, the robot must be able to identify the bumper locations of vehicles in the parking area and then precisely position the LPR camera relative to the bumper. This paper describes a 2D-laser scanner based approach to bumper identification and laser servoing for the T4 robot. The system uses a gimbal-mounted scanning laser. As the T4 robot travels down a row of parking stalls, data is collected from the laser every 100ms. For each parking stall in the range of the laser during the scan, the data is matched to a ``bumper box'' corresponding to where a car bumper is expected, resulting in a point cloud of data corresponding to a vehicle bumper for each stall. Next, recursive line-fitting algorithms are used to determine a line for the data in each stall's ``bumper box.'' The fitting technique uses Hough based transforms, which are robust against segmentation problems and fast enough for real-time line fitting. Once a bumper line is fitted with an acceptable confidence, the bumper location is passed to the T4 motion controller, which moves to position the LPR camera properly relative to the bumper. The paper includes examples and results that show the effectiveness of the technique, including its ability to work in real-time.

  10. Time-dependent inhibition and estimation of CYP3A clinical pharmacokinetic drug-drug interactions using plated human cell systems.

    PubMed

    Albaugh, Daniel R; Fullenwider, Cody L; Fisher, Michael B; Hutzler, J Matthew

    2012-07-01

    The current studies assessed the utility of freshly plated hepatocytes, cryopreserved plated hepatocytes, and cryopreserved plated HepaRG cells for the estimation of inactivation parameters k(inact) and K(I) for CYP3A. This was achieved using a subset of CYP3A time-dependent inhibitors (fluoxetine, verapamil, clarithromycin, troleandomycin, and mibefradil) representing a range of potencies. The estimated k(inact) and K(I) values for each time-dependent inhibitor were compared with those obtained using human liver microsomes and used to estimate the magnitude of clinical pharmacokinetic drug-drug interaction (DDI). The inactivation kinetic parameter, k(inact), was most consistent across systems tested for clarithromycin, verapamil, and troleandomycin, with a high k(inact) of 0.91 min(-1) observed for mibefradil in HepaRG cells. The apparent K(I) estimates derived from the various systems displayed a range of variability from 3-fold for clarithromycin (5.4-17.7 μM) to 6-fold for verapamil (1.9-12.6 μM). In general, the inactivation kinetic parameters derived from the cell systems tested fairly replicated what was observed in time-dependent inhibition studies using human liver microsomes. Despite some of the observed differences in inactivation kinetic parameters, the estimated DDIs derived from each of the tested systems generally agreed with the clinically reported DDI within approximately 2-fold. In addition, a plated cell approach offered the ability to conduct longer primary incubations (greater than 30 min), which afforded improved ability to identify the weak time-dependent inhibitor fluoxetine. Overall, results from these studies suggest that in vitro inactivation parameters generated from plated cell systems may be a practical approach for identifying time-dependent inhibitors and for estimating the magnitude of clinical DDIs.

  11. Forced vibrations of plates and cylindrical shells with regular orthogonal system of stiffeners

    NASA Astrophysics Data System (ADS)

    Efimtsov, B. M.; Lazarev, L. A.

    2009-10-01

    A wide range of engineering structures, such as aircraft fuselages or ship hulls have as the foundation a shell orthogonally strengthened by two sets of stiffeners. Solution of the task related to determining the vibrations of such complicated structures requires an application of special methods which permit accounting for the interaction between the shell and the two sets of discrete stiffeners correctly. The present work proposes an effective method of predicting the vibrations of a finite orthogonally stiffened structure as a part of an infinite one when the edge conditions permit. The prediction method proposed is based on the method of space-harmonic expansions when the shell displacements and forces are presented in the form of special double trigonometric series. The method allows the interconnection of all three components of displacement and rotation of the shell and the stiffeners to be taken into account. The vibration velocity of the construction is determined directly without a need for solving the task of eigen-values first. The vibration shapes are broken into a large number of non-interacting groups of shapes. The solution reduces to a system of equations relating to the generalized reactions at supports. All this allows predictions to be made for large parts of the investigated construction over practically the whole frequency range of sound.

  12. Detection system for inclusion defects in hot-rolled steel plates using MFLT with two different magnetizing strengths

    NASA Astrophysics Data System (ADS)

    Yotsuji, Junichi; Koshihara, Takahiro

    2014-02-01

    Recently, steel can manufacturing requires higher quality because otherwise minute non-metallic inclusions in thin sheets cause cracks and result in a burst during pressurization after the pressing process. Quality testing systems have already been installed in the final process in steel plants, but if there were another inspection in an earlier step, for example, at the hot strip mill, the mass manufacture of nonconforming products could be avoided and maintaining quality control would be more efficient. In order to detect inclusion defects in hot-rolled steel plates, the authors developed a new technique for MFLT (Magnetic Flux Leakage Testing) using different magnetizing forces. According to an analysis of the noise factors in MFLT, it was found that the signals generated from the scale layer on a steel surface are dominant. A different magnetizing force method is the used to decrease this overpowering noise level in MFLT. In this paper, it was confirmed that inclusions larger than 160μm in diameter and less than 0.45mm in depth can be detected utilizing this method.

  13. Study on detecting spatial distribution of neutrons and gamma rays using a multi-imaging plate system.

    PubMed

    Tanaka, Kenichi; Sakurai, Yoshinori; Endo, Satoru; Takada, Jun

    2014-06-01

    In order to measure the spatial distributions of neutrons and gamma rays separately using the imaging plate, the requirement for the converter to enhance specific component was investigated with the PHITS code. Consequently, enhancing fast neutrons using recoil protons from epoxy resin was not effective due to high sensitivity of the imaging plate to gamma rays. However, the converter of epoxy resin doped with (10)B was found to have potential for thermal and epithermal neutrons, and graphite for gamma rays.

  14. Improved Thermal-Vacuum Compatible Flat Plate Radiometric Souce for System-Level Testing of Optical Sensors

    NASA Technical Reports Server (NTRS)

    Schwarz, Mark A.; Kent, Craig J.; Bousquet, Robert; Brown, Steven W.

    2015-01-01

    This work describes the development of an improved vacuum compatible flat plate radiometric source used for characterizing and calibrating remote optical sensors, in situ, throughout their testing period. The original flat plate radiometric source was developed for use by the VIIRS instrument during the NPOESS Preparatory Project (NPP). Following this effort, the FPI has had significant upgrades in order to improve both the radiometric throughput and uniformity. Results of the VIIRS testing with the reconfigured FPI are reported and discussed.

  15. Near Fault Observatories (NFO) services and integration plan for European Plate Observing System (EPOS) Implementation Phase

    NASA Astrophysics Data System (ADS)

    Chiaraluce, Lauro

    2016-04-01

    the services provided by other Thematic Core Services for the standard data (e.g. seismic and geodetic) and on the direct access to the e-infrastructures of individual NFOs via the Integrated Core Services web services for access and distribution of non standard data (e.g. strain- and tilt-meters, geochemical and electro- magneto-telluric data). We will collaborate with the other groups possessing the same data on data harmonization in terms of both format and metadata description to optimise and facilitate the integration and interoperability processes. The services will include a Virtual Laboratory, novel visualization tools for data and products describing the anatomy of active faults and the physical processes governing earthquake generation. VL is an online engagement and knowledge sharing initiative for communicating to the other scientists, stockholders and the public the state of scientific knowledge concerning earthquake source and tectonic processes generating catastrophic events. The availability of real-time data provides the unique opportunity of observing all phases of the earthquake rupture. It is thus of crucial importance developing methodologies to follow in real-time the evolution of the event (e.g. Earthquake Early Warning systems). NFOs are ideal infrastructures for hosting testing centers where a variety of scientific algorithms for real-time monitoring can be independently evaluated. Besides the interest for fundamental science, such developments have a societal impact and can attract new stakeholders such as industry partners who are interested in adopting in such (e.g. EEW) technologies.

  16. The European Plate Observing System (EPOS): Integrating Thematic Services for Solid Earth Science

    NASA Astrophysics Data System (ADS)

    Atakan, Kuvvet; Bailo, Daniele; Consortium, Epos

    2016-04-01

    The mission of EPOS is to monitor and understand the dynamic and complex Earth system by relying on new e-science opportunities and integrating diverse and advanced Research Infrastructures in Europe for solid Earth Science. EPOS will enable innovative multidisciplinary research for a better understanding of the Earth's physical and chemical processes that control earthquakes, volcanic eruptions, ground instability and tsunami as well as the processes driving tectonics and Earth's surface dynamics. Through integration of data, models and facilities EPOS will allow the Earth Science community to make a step change in developing new concepts and tools for key answers to scientific and socio-economic questions concerning geo-hazards and geo-resources as well as Earth sciences applications to the environment and to human welfare. EPOS, during its Implementation Phase (EPOS-IP), will integrate multidisciplinary data into a single e-infrastructure. Multidisciplinary data are organized and governed by the Thematic Core Services (TCS) and are driven by various scientific communities encompassing a wide spectrum of Earth science disciplines. These include Data, Data-products, Services and Software (DDSS), from seismology, near fault observatories, geodetic observations, volcano observations, satellite observations, geomagnetic observations, as well as data from various anthropogenic hazard episodes, geological information and modelling. In addition, transnational access to multi-scale laboratories and geo-energy test-beds for low-carbon energy will be provided. TCS DDSS will be integrated into Integrated Core Services (ICS), a platform that will ensure their interoperability and access to these services by the scientific community as well as other users within the society. This requires dedicated tasks for interactions with the various TCS-WPs, as well as the various distributed ICS (ICS-Ds), such as High Performance Computing (HPC) facilities, large scale data storage

  17. Multiplexed chemiluminescent assays in ArrayPlates for high-throughput measurement of gene expression

    NASA Astrophysics Data System (ADS)

    Martel, Ralph R.; Rounseville, Matthew P.; Botros, Ihab W.; Seligmann, Bruce E.

    2002-06-01

    Multiplexed Molecular Profiling (MMP) assays for drug discovery are performed in ArrayPlates. ArrayPlates are 96- well microtiter plates that contain a 16-element array at the bottom of each well. Each element within an array measures one analyte in a sample. A CCD imager records the quantitative chemiluminescent readout of all 1,536 elements in a 96-well plate simultaneously. Since array elements are reagent modifiable by the end-user, ArrayPlates can be adapted to a broad range of nucleic acid- and protein-based assays. Such multiplexed assays are rapidly established, flexible, robust, automation-friendly and cost-effective. Nucleic acid assays in ArrayPlates can detect DNA and RNA, including SNPs and ESTs. A multiplexed mRNA assay to measure the expression of 16 genes is described. The assay combines a homogeneous nuclease protection assay with subsequent probe immobilization to the array by means of a sandwich hybridization followed with chemiluminescent detection. This assay was used to examine cells grown and treated in microplates and avoided cloning, transfection, RNA insolation, reverse transcription, amplification and fluorochrome labeling. Standard deviations for the measurement of 16 genes ranged from 3 percent to 13 percent in samples of 30,000 cells. Such ArrayPlates transcription assays are useful in drug discovery and development for target validation, screening, lead optimization, metabolism and toxicity profiling. Chemiluminescent detection provides ArrayPlates assays with high signal-to-noise readout and simplifies imager requirements. Imaging a 2D surface that contains arrays simplifies lens requirements relative to imaging columns of liquid in microtiter plate wells. The Omix imager for ArrayPlates is described.

  18. Plating Processes Utilizing High Intensity Acoustic Beams

    NASA Technical Reports Server (NTRS)

    Oeftering, Richard C. (Inventor); Denofrio, Charles (Inventor)

    2002-01-01

    A system and a method for selective plating processes are disclosed which use directed beams of high intensity acoustic waves to create non-linear effects that alter and improve the plating process. The directed beams are focused on the surface of an object, which in one embodiment is immersed in a plating solution, and in another embodiment is suspended above a plating solution. The plating processes provide precise control of the thickness of the layers of the plating, while at the same time, in at least some incidents, eliminates the need for masking.

  19. Secretion of albumin and induction of CYP1A2 and CYP3A4 in novel three-dimensional culture system for human hepatocytes using micro-space plate.

    PubMed

    Nishimura, Masuhiro; Hagi, Mieko; Ejiri, Yoko; Kishimoto, Sanae; Horie, Toru; Narimatsu, Shizuo; Naito, Shinsaku

    2010-01-01

    We evaluated a novel primary three-dimensional culture system for human hepatocytes using micro-space plates. The functional activity of human hepatocytes in primary culture was determined by measuring albumin secretion from hepatocytes to medium and measuring expression levels of albumin, CYP1A2 and CYP3A4 mRNA. Albumin secretion was higher in micro-space plates compared with traditional plates after 72 h of culture; the levels of albumin secretion from hepatocytes to medium in culture using micro-space plates after 96 h of culture were 2.7-fold higher than those in culture using traditional plates, and secretion of albumin in micro-space plate culture subsequently remained constant. Expression levels of albumin, CYP1A2 and CYP3A4 mRNA in the culture of hepatocytes were significantly higher using micro-space plates than using traditional plates. The inducibility of CYP1A2 and CYP3A4 mRNA after exposure to inducers in hepatocyte culture on micro-space plates was comparable to that in culture on traditional plates, while expression of CYP1A2 and CYP3A4 mRNA after exposure to inducers was higher on micro-space plates than on traditional plates. The present study demonstrates that a novel primary three-dimensional culture system of cryopreserved human hepatocytes using micro-space plates could be used for evaluating the induction of drug-metabolizing enzymes in humans. This in vitro method may thus be useful for screening the induction potency of new drug candidates.

  20. A novel integration system of magnetically immobilized cells and a pair of graphite plate-stainless iron mesh electrodes for the bioremediation of coking wastewater.

    PubMed

    Jiang, Bei; Tan, Liang; Ning, Shuxiang; Shi, Shengnan

    2016-09-01

    Magnetically immobilized cells of Comamonas sp. JB coupling with electrode reaction was developed to enhance the treatment efficiency of coking wastewater containing phenol, carbazole (CA), dibenzofuran (DBF), and dibenzothiophene (DBT). The pair of graphite plate-stainless iron mesh electrodes was chosen as the most suitable electrodes. Magnetically immobilized cells coupling with graphite plate-stainless iron mesh electrodes (coupling system) exhibited high degradation activity for all the compounds, which were significantly higher than the sum by single magnetically immobilized cells and electrode reaction at the optimal voltage. Recycling experiments demonstrated that the degradation activity of coupling system increased gradually during eight recycles, indicating that there was a coupling effect between the biodegradation and electrode reaction. Phenol hydroxylase and qPCR assays confirmed that appropriate electrical stimulation could improve phenol hydroxylase activity and promote cells growth. Toxicity assessment suggested the treatment of the coking wastewater by coupling system led to less toxicity than untreated wastewater.

  1. Downgoing plate controls on overriding plate deformation in subduction zones

    NASA Astrophysics Data System (ADS)

    Garel, Fanny; Davies, Rhodri; Goes, Saskia; Davies, Huw; Kramer, Stephan; Wilson, Cian

    2014-05-01

    Although subduction zones are convergent margins, deformation in the upper plate can be extensional or compressional and tends to change through time, sometimes in repeated episodes of strong deformation, e.g, phases of back-arc extension. It is not well understood what factors control this upper plate deformation. We use the code Fluidity, which uses an adaptive mesh and a free-surface formulation, to model a two-plate subduction system in 2-D. The model includes a composite temperature- and stress-dependent rheology, and plates are decoupled by a weak layer, which allows for free trench motion. We investigate the evolution of the state of stress and topography of the overriding plate during the different phases of the subduction process: onset of subduction, free-fall sinking in the upper mantle and interaction of the slab with the transition zone, here represented by a viscosity contrast between upper and lower mantle. We focus on (i) how overriding plate deformation varies with subducting plate age; (ii) how spontaneous and episodic back-arc spreading develops for some subduction settings; (iii) the correlation between overriding plate deformation and slab interaction with the transition zone; (iv) whether these trends resemble observations on Earth.

  2. Comparison of diploid fibroblast and rabbit kidney tissue cultures and a diploid fibroblast microtiter plate system for the isolation of herpes simplex virus.

    PubMed Central

    Langenberg, A; Zbanyszek, R; Dragavon, J; Ashley, R; Corey, L

    1988-01-01

    We evaluated the relative sensitivities of two cell systems (rabbit kidney [RK] and human diploid fibroblast [DF; human embryonic tonsil]) in standard tube cultures versus DF cells in a 48-well microtiter plate system for the detection of both symptomatic and asymptomatic herpes simplex virus (HSV) infection. At least one system isolated HSV in 111 of 809 specimens (13.7%). HSV was isolated in RK tube cultures from 110 specimens (99%), in DF tube cultures from 91 specimens (82%), and in DF microtiter plates from 95 specimens (86%). The frequency of HSV isolation varied with the anatomic site and the presence or absence of a herpetic lesion. The sensitivities of the three culture systems remained similar whether the specimens were obtained from lesions or whether the specimens were taken to determine if asymptomatic excretion of HSV was present. While RK tube cultures were more sensitive than DF tube cultures, the DF microtiter plate system was as sensitive as DF tube cultures and its use is supported as a cheaper and less labor-intensive method for the detection of HSV. PMID:2846647

  3. Impact on plate waste of switching from a tray to a trayless delivery system in a university dining hall and employee response to the switch.

    PubMed

    Thiagarajah, Krisha; Getty, Victoria M

    2013-01-01

    A potential strategy for decreasing food waste in foodservice operations is trayless dining. The objective of this 2010 study was to compare the impact of using a tray vs a trayless system on plate waste and on employees' attitudes. To test the hypothesis that going trayless would reduce waste, liquid and solid plate waste were measured for 1 week with the then-existing tray system and again after a new trayless system was implemented in a buffet-style university dining hall serving roughly1,000 meals a day. Foodservice staff were invited to participate in a focus group about the impact on their jobs. The investigators calculated plate waste per patron under the two systems and used an independent samples t test to examine the significance of the difference. Comments from the focus group were analyzed for themes. A significant decrease in solid waste per patron (0.81 oz; P=0.001) was observed in switching from the tray to the trayless system (4.39 ± 0.24 oz vs 3.58 ± 0.08 oz per patron). A nonsignificant reduction was observed with liquid waste (49.77 ± 2.62 mL vs 46.36 ± 4.51 mL; P=0.18). Most of the employees preferred the trayless system as long as it did reduce waste, but felt that increased breakage of dishware and increased need to wipe down tables were possible concerns resulting from the switch. This study demonstrates that trayless dining can reduce plate waste, and that employees can be supportive of the change.

  4. Comparison of base of support size during gait initiation using force-plate and motion-capture system: A Bland and Altman analysis.

    PubMed

    Yiou, E; Teyssèdre, C; Artico, R; Fourcade, P

    2016-12-08

    This study aimed to estimate the error made by investigators when force-plate data are used to approximate base of support size during gait initiation. Step length and step width obtained with a method based on motion capture system (Kinematics method, considered the "gold standard") and with a method based on the centre of pressure traces obtained from a force-plate (Force-plate method) were purposely compared using descriptive statistics and the Bland and Altman (BA) method. Participants (N=19) performed series of gait initiation in Spontaneous and Maximal Velocity Conditions (SVC and MVC, respectively). BA analysis showed that 1) step length and width biases, corresponding to the difference between the two methods, were very small (<2.1%) in both velocity conditions and 2) the 95% limits of agreement of the BA plots ranged between 10% and 15% in absolute value. Repeated measures ANOVA showed that step length was significantly larger in MVC than in SVC, with no velocity X method interaction. There was no significant effect of the method on both step parameters. The present results suggest that the Force-plate method is sufficiently accurate to compare step parameters across conditions. However, researchers should be aware that non-negligible errors might occur when considering individual data.

  5. Improved Thermal-Vacuum Compatible Flat Plate Radiometric Source For System-Level Testing Of Optical Sensors

    NASA Technical Reports Server (NTRS)

    Schwarz, Mark A.; Kent, Craig J.; Bousquet, Robert; Brown, Steven W.

    2016-01-01

    In this work, we describe an improved thermal-vacuum compatible flat plate radiometric source which has been developed and utilized for the characterization and calibration of remote optical sensors. This source is unique in that it can be used in situ, in both ambient and thermal-vacuum environments, allowing it to follow the sensor throughout its testing cycle. The performance of the original flat plate radiometric source was presented at the 2009 SPIE1. Following the original efforts, design upgrades were incorporated into the source to improve both radiometric throughput and uniformity. The pre-thermal-vacuum (pre-TVAC) testing results of a spacecraft-level optical sensor with the improved flat plate illumination source, both in ambient and vacuum environments, are presented. We also briefly discuss potential FPI configuration changes in order to improve its radiometric performance.

  6. Improved thermal-vacuum compatible flat plate radiometric source for system-level testing of remote optical sensors

    NASA Astrophysics Data System (ADS)

    Schwarz, Mark A.; Kent, Craig J.; Bousquet, Robert; Brown, Steven W.

    2016-09-01

    In this work, we describe an improved thermal-vacuum compatible flat plate radiometric source which has been developed and utilized for the characterization and calibration of remote optical sensors. This source is unique in that it can be used in situ, in both ambient and thermal-vacuum environments, allowing it to follow the sensor throughout its testing cycle. The performance of the original flat plate radiometric source was presented at the 2009 SPIE1. Following the original efforts, design upgrades were incorporated into the source to improve both radiometric throughput and uniformity. The pre-thermal-vacuum (pre-TVAC) testing results of a spacecraft-level optical sensor with the improved flat plate illumination source, both in ambient and vacuum environments, are presented. We also briefly discuss potential FPI configuration changes in order to improve its radiometric performance. Keywords: Calibration, radiometry, remote sensing, source.

  7. Plating versus intramedullary fixation for mid-shaft clavicle fractures: a systemic review and meta-analysis.

    PubMed

    Gao, Yan; Chen, Wei; Liu, Yue-Jv; Li, Xu; Wang, Hai-Li; Chen, Zhao-Yu

    2016-01-01

    Background. Plate fixation and intramedullary fixation are the most commonly used surgical treatment options for mid-shaft clavicle fractures; the latter method has demonstrated better performance in some studies. Objectives. Our aim was to critically review and summarize the literature comparing the outcomes of mid-shaft clavicle fracture treatment with plate fixation or intramedullary fixation to identify the better approach. Search Methods. Potential academic articles were identified from the Cochrane Library, MEDLINE (1966-2015.5), PubMed (1966-2015.5), EMBASE (1980-2015.5) and ScienceDirect (1966-2015.5). Gray studies were identified from the references of the included literature. Selection Criteria. Randomized controlled trials (RCTs) and non-RCTs comparing plate fixation and intramedullary fixation for mid-shaft clavicle fracture were included. Data Collection and Analysis. Two reviewers performed independent data abstraction. The I (2) statistic was used to assess heterogeneity. A fixed- or random-effects model was used for the meta-analysis. Results. Six RCTs and nine non-RCTs were retrieved, including 513 patients in the intramedullary fixation group and 521 patients in the plating group. No significant differences in terms of the union rate and shoulder function were found between the groups. Patients in the intramedullary fixation group had a shorter operative time, less blood loss, smaller wound size, and shorter union time than those in the plating group. With respect to complications, significant differences were identified for all complications and major complications (wound infection, nonunion, implant failures, transient brachial plexopathy, and pain after 6 months). Similar secondary complications (symptomatic hardware, hardware irritation, prominence, numbness, hypertrophic callus) were observed in both groups. Conclusions. Intramedullary fixation may be superior to plate fixation in the treatment of mid-shaft clavicle fractures, with similar

  8. FIZICS: fluorescent imaging zone identification system, a novel macro imaging system.

    PubMed

    Skwish, Stephen; Asensio, Francisco; King, Greg; Clarke, Glenn; Kath, Gary; Salvatore, Michael J; Dufresne, Claude

    2004-12-01

    Constantly improving biological assay development continues to drive technological requirements. Recently, a specification was defined for capturing white light and fluorescent images of agar plates ranging in size from the NUNC Omni tray (96-well footprint, 128 x 85 mm) to the NUNC Bio Assay Dish (245 x 245 mm). An evaluation of commercially available products failed to identify any system capable of fluorescent macroimaging with discrete wavelength selection. To address the lack of a commercially available system, a custom imaging system was designed and constructed. This system provides the same capabilities of many commercially available systems with the added ability to fluorescently image up to a 245 x 245 mm area using wavelengths in the visible light spectrum.

  9. Absorbable plate strength loss during molding.

    PubMed

    Ballard, Tiffany N S; Kelly, Kevin J; Zaydfudim, Victor; Walcutt, Noah L; Lahijani, Soheil S; Shack, R Bruce; Thayer, Wesley P

    2010-05-01

    Bioabsorbable plating systems play an integral role in cranial vault remodeling. After experiencing a case of plate failure requiring emergent reexploration, we investigated the potential causes. We hypothesize that extended submersion in the molding bath during plate preparation might advance the rate of hydrolysis and compromise plate structural integrity. Using an absorbable poly-D/L-lactic acid plating system, we assessed the effect of extended submersion on plate strength and stiffness when loaded in a cantilever fashion and with pure tension. We assessed these differences with the Student t test and linear regression modeling. We also generated a computer model of the plates for finite element analysis. When left in the molding bath for extended periods, the plates changed color and lost strength. After 5 minutes, 30% of maximum plate load capacity was lost in a cantilever beam test (P < 0.001) consistent with use of a 15% thinner plate. Tensile testing revealed the initial elastic modulus of 6.42 +/- 0.13 GPa decreased 16% to 5.41 +/- 0.50 GPa after 5 minutes of submersion (P = 0.027). The changes in plate strength and elastic modulus both worsened with increased submersion times. Finite element analysis of the plates also predicted clinically significant increases in plate deviation under normal loading conditions. Our study demonstrates that extended submersion of absorbable plates during molding results in a significant loss of plate strength and stiffness. Further, our computer model predicts that these changes could result in an unacceptable plate deviation under normal loading conditions. Together, these data caution against overmolding of plates to avoid compromising their structural integrity.

  10. Establishment of a fully automated microtiter plate-based system for suspension cell culture and its application for enhanced process optimization.

    PubMed

    Markert, Sven; Joeris, Klaus

    2017-01-01

    We developed an automated microtiter plate (MTP)-based system for suspension cell culture to meet the increased demands for miniaturized high throughput applications in biopharmaceutical process development. The generic system is based on off-the-shelf commercial laboratory automation equipment and is able to utilize MTPs of different configurations (6-24 wells per plate) in orbital shaken mode. The shaking conditions were optimized by Computational Fluid Dynamics simulations. The fully automated system handles plate transport, seeding and feeding of cells, daily sampling, and preparation of analytical assays. The integration of all required analytical instrumentation into the system enables a hands-off operation which prevents bottlenecks in sample processing. The modular set-up makes the system flexible and adaptable for a continuous extension of analytical parameters and add-on components. The system proved suitable as screening tool for process development by verifying the comparability of results for the MTP-based system and bioreactors regarding profiles of viable cell density, lactate, and product concentration of CHO cell lines. These studies confirmed that 6 well MTPs as well as 24 deepwell MTPs were predictive for a scale up to a 1000 L stirred tank reactor (scale factor 1:200,000). Applying the established cell culture system for automated media blend screening in late stage development, a 22% increase in product yield was achieved in comparison to the reference process. The predicted product increase was subsequently confirmed in 2 L bioreactors. Thus, we demonstrated the feasibility of the automated MTP-based cell culture system for enhanced screening and optimization applications in process development and identified further application areas such as process robustness. The system offers a great potential to accelerate time-to-market for new biopharmaceuticals. Biotechnol. Bioeng. 2017;114: 113-121. © 2016 Wiley Periodicals, Inc.

  11. Corrugated cover plate for flat plate collector

    DOEpatents

    Hollands, K. G. Terry; Sibbitt, Bruce

    1978-01-01

    A flat plate radiant energy collector is providing having a transparent cover. The cover has a V-corrugated shape which reduces the amount of energy reflected by the cover away from the flat plate absorber of the collector.

  12. Thermal protection system gap heating rates of the Rockwell International flat plate heat transfer model (OH2A/OH2B)

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Lockman, W. K.; Grifall, W. J.

    1973-01-01

    Heat transfer data for the Rockwell International Flat Plate Thermocouple Model are presented. The model simulated the Space Shuttle Vehicle Thermal Protection System. Data were recorded for locations in and around various size gaps for various gap orientation configurations. The test was conducted at Mach 5.1 for free-stream Reynolds number per foot values from 500,000 to 1,500,000.

  13. Growth Plate Fractures

    MedlinePlus

    .org Growth Plate Fractures Page ( 1 ) The bones of children and adults share many of the same risks for injury. But because they ... to a unique injury called a growth plate fracture. Growth plates are areas of cartilage located near ...

  14. Structure of the Lithosphere-Asthenosphere System Beneath the Juan de Fuca Plate: Results of Body Wave Imaging Using Cascadia Initiative Data

    NASA Astrophysics Data System (ADS)

    Byrnes, J. S.; Toomey, D. R.; Hooft, E. E. E.

    2014-12-01

    The plate-scale deployment of ocean bottom seismometers (OBS) as part of the Cascadia Initiative (CI) of NSF provides a unique opportunity to study the structure and dynamics of the lithosphere-asthenosphere system beneath an entire oceanic plate, from its birth at a spreading center to its subduction beneath a continent. Here we present tomographic images of the seismic structure of oceanic upper mantle beneath the Juan de Fuca (JdF) and Gorda plates derived from body wave delay times. The results constrain structural anomalies beneath the JdF and Gorda spreading centers, the Blanco and Mendocino transform faults, near ridge hotspots such as Axial Seamount, and the upper mantle structure beneath the subducting oceanic lithosphere. We measured delay times of teleseismic P and S wave phases for the first two years of the CI. Our tomographic analysis assumes both isotropic and anisotropic starting models and accounts for finite-frequency effects and three-dimensional ray bending. Preliminary results indicate that the upper mantle structure beneath the JdF spreading center is asymmetric, with lower shear wave velocities beneath the Pacific plate (also the direction of ridge migration). On a regional scale, regions of lower seismic velocities beneath the JdF and Gorda spreading centers correlate with shallower ridge depths. Beneath the southern Gorda plate a low velocity anomaly is detected, which is absent to the north; this anomaly is bounded to the south by the Mendocino transform. Ongoing work includes analysis of the third year of CI data, which will improve resolution of structure and allow better definition of anomalies in the vicinity of the Blanco transform. In addition, we will combine ocean and continental data to obtain images of the Cascadia subduction zone.

  15. Shear wave splitting observations across the Juan de Fuca plate system: Ridge- to-trench constraints on mantle flow from 2 years of Cascadia Initiative OBS data

    NASA Astrophysics Data System (ADS)

    Bodmer, M.; Toomey, D. R.; Hooft, E. E. E.

    2014-12-01

    We present SKS splitting measurements for the first two years of data collected by the Cascadia Initiative (CI) amphibious array. Our analysis includes observations from over 100 ocean bottom seismometers (OBS), as well as 31 onshore stations, and spans both the Juan de Fuca and Gorda plates. The CI dataset is unique in that it includes several regions that can distinctly influence anisotropic fabric development such as: the upwelling mantle beneath the Juan de Fuca and Gorda ridges, the young evolving oceanic lithosphere of the plate interior, the Blanco transform fault, and the Cascadia subduction zone. For the first time, we are able to analyze these regions with a single dataset, and using a common methodology. Splitting measurements are routinely done on land sites, but have been completed on relatively few OBS stations. This is largely due to the low signal to noise present in OBS data, which can obscure the splitting results. To address that nearly all the OBS data exceeds the global high noise limit at the frequencies used for splitting, we implement a rigorous quality control scheme. Our method specifically takes into account the response of common splitting methods to high noise data and addresses known issues such as cycle skipping, false minima, low transverse energy, and near-null measurements. Individual measurements are filtered at 0.03-0.1 Hz, manually checked for quality, and stacked. Preliminary results show trench perpendicular onshore measurements consistent with previous studies. Oceanic measurements in the plate interior show a coherent fast axis roughly aligned with absolute plate motion. Several measurements near the ridge and trench appear to be rotated in the ridge and trench parallel directions. Continuing work will integrate splitting measurements from the final two years of the CI with these findings, which will be used to characterize the ridge-to-trench mantle flow across the Juan de Fuca plate system.

  16. Wetting reaction of Sn-Ag based solder systems on Cu substrates plated with Au and/or Pd layer

    NASA Astrophysics Data System (ADS)

    Liu, C. Y.; Li, Jian; Vandentop, G. J.; Choi, W. J.; Tu, K. N.

    2001-05-01

    The wetting behavior of SnAg based Pb-free solders on Cu and Cu substrates plated with Au, Pd, and Au/Pd thin films have been studied. The wetting angle and kinetics of interfacial reaction were measured. The Au-plated substrates exhibit better wetting than the Pd-plated substrates. In the case of SnAg on Pd-plated Cu, SEM observation revealed that the solder cap was surrounded by an innerring of Cu-Sn compound and an outer ring of Pd-Sn compound. This implies that the molten SnAg solder had removed the Pd and wetted the Cu directly in the equilibrium state. The effects of pre-doping Cu in the SnAg solder on wetting behavior were also investigated. We found that wettability decreases with increasing Cu content in the solder. We also observed that the SnAgCu solders have a lower Cu consumption rate than the SnAg solder.

  17. Kinematic model for out-of-sequence thrusting: Motion of two ramp-flat faults and the production of upper plate duplex systems

    NASA Astrophysics Data System (ADS)

    Pavlis, Terry L.

    2013-06-01

    Kinematic models developed here suggest a bewildering array of structural styles can be generated during out-of-sequence thrusting. Many of these structures would be difficult to distinguish from a normally stacked thrust sequence and the process can produce younger-on-older faults that could easily be misinterpreted as normal faults. This paper considers a small subset of this problem within a large model space by considering structures that develop along a pair of ramp-flat faults that are moving simultaneously, or sequentially. Motion on the lower ramp warps the structurally higher fault due to fault-bend folding and when the fault ruptures through the warp it transfers a horse to the upper hanging wall. Continuity of the process generates what is referred to here as an "upper plate duplex" to distinguish the structure from a conventional duplex. Kinematic parameters are developed for two models within this general problem: 1) a system with a fixed ramp in the lower thrust, overridden by an upper thrust; and 2) a double-duplex system where a conventional duplex develops along the lower fault at the same time as an upper plate duplex is formed along the upper fault. The theory is tested with forward models using 2D Move software and these tests indicate different families of structural styles form in association with relative scaling of ramp systems, slip-ratio between faults, and aspect ratios of horse blocks formed in the upper-plate duplex. A first-order result of the analysis is that an upper plate duplex can be virtually indistinguishable from a conventional duplex unless the trailing branch lines of the horses are exposed or imaged; a condition seldom met in natural exposures. Restoration of an upper-plate duplex produces counterintuitive fault geometry in the restored state, and thus, restorations of upper plate duplexes that erroneously assume a conventional duplex model would produce restored states that are seriously in error. In addition, in most of

  18. Robotic platform for parallelized cultivation and monitoring of microbial growth parameters in microwell plates.

    PubMed

    Knepper, Andreas; Heiser, Michael; Glauche, Florian; Neubauer, Peter

    2014-12-01

    The enormous variation possibilities of bioprocesses challenge process development to fix a commercial process with respect to costs and time. Although some cultivation systems and some devices for unit operations combine the latest technology on miniaturization, parallelization, and sensing, the degree of automation in upstream and downstream bioprocess development is still limited to single steps. We aim to face this challenge by an interdisciplinary approach to significantly shorten development times and costs. As a first step, we scaled down analytical assays to the microliter scale and created automated procedures for starting the cultivation and monitoring the optical density (OD), pH, concentrations of glucose and acetate in the culture medium, and product formation in fed-batch cultures in the 96-well format. Then, the separate measurements of pH, OD, and concentrations of acetate and glucose were combined to one method. This method enables automated process monitoring at dedicated intervals (e.g., also during the night). By this approach, we managed to increase the information content of cultivations in 96-microwell plates, thus turning them into a suitable tool for high-throughput bioprocess development. Here, we present the flowcharts as well as cultivation data of our automation approach.

  19. Ultramicroband array electrode. 1. Analysis of mercury in contaminated soils and flue gas exposed samples using a gold-plated iridium portable system by anodic stripping voltammetry.

    PubMed

    Xiao, Li; Dietze, William; Nyasulu, Frazier; Mibeck, Blaise A F

    2006-07-15

    The applicability of a gold-plated iridium Nano-Band array ultramicroelectrode (6 microm by 0.2 microm, 64-microm interspacing, 100 electrode bands) in the analysis of mercury using a portable system is demonstrated by anodic stripping voltammetry in real-life samples. Optimized measurement parameters, 0.1 M HCl electrolyte, plating potential of 0 mV, and staircase scan mode were identified. The dynamic linear range is 10-180 ppb at 5-s deposition time with 1.5 microC of gold plated. The experimental detection limit for Hg2+ in 0.1 M HCl was 0.5 ppb at a deposition time of 4 min and a scan rate of 10 V/s. Real-life samples, such as flue gas exposed samples from flue gas simulators could be analyzed within 5 min using the method of standard additions. We identified a field-portable extraction procedure for soil samples using 1:1 concentrated HNO3/30% H2O2 mixture, compatible with ASV and the iridium electrode. The detection limit for soils is 1 ppm. The results obtained using ASV are in good agreement with reference values using cold vapor atomic absorption for the sample matrixes studied here. To our knowledge, this is the first mercury application using a reusable iridium array ultramicroelectrode. The portable potentiostat is less than 500 g, and together with the portable digestion method, makes the Nano-Band Explorer system field applicable.

  20. Laboratory flume experiments with the Swiss plate geophone bed load monitoring system: 2. Application to field sites with direct bed load samples

    NASA Astrophysics Data System (ADS)

    Wyss, Carlos R.; Rickenmann, Dieter; Fritschi, Bruno; Turowski, Jens M.; Weitbrecht, Volker; Travaglini, Eric; Bardou, Eric; Boes, Robert M.

    2016-10-01

    The Swiss plate geophone is a bed load surrogate monitoring system that had been calibrated in several gravel bed streams through field calibration measurements. Field calibration measurements are generally expensive and time consuming, therefore we investigated the possibility to replace it by a flume-based calibration approach. We applied impulse-diameter relations for the Swiss plate geophone obtained from systematic flume experiments to field calibration measurements in four different gravel bed streams. The flume-based relations were successfully validated with direct bed load samples from field measurements, by estimating the number of impulses based on observed bed load masses per grain-size class. We estimated bed load transport mass by developing flume-based and stream-dependent calibration procedures for the Swiss plate geophone system using an additional empirical function. The estimated masses are on average in the range of ±90% of measured bed load masses in the field, but the accuracy is generally improved for larger transported bed load masses. We discuss the limitations of the presented flume-based calibration approach.

  1. Geodynamics of flat-slab subduction, sedimentary basin development, and hydrocarbon systems along the southern Alaska convergent plate margin

    NASA Astrophysics Data System (ADS)

    Finzel, Emily S.

    Combining field-based geologic studies and numerical modeling provides a robust tool for evaluating the geodynamics of convergent margins. Southern Alaska is arguably the most tectonically active part of the convergent margin of western North America. This conceptual approach has been used to interpret the modern basin dynamics, as well as key stages in the Cenozoic development of this region, including spreading-ridge and flat-slab subduction. New macrofossil, palynological, and lithostratigraphic data for the Bear Lake Formation in the Bristol Bay retroarc basin allow us to construct the first chronostratigraphic framework for this formation, and indicate deposition during Middle and Late Miocene time in a regional transgressive estuarine depositional system. In the Cook Inlet forearc basin, new detrital zircon U-Pb geochronology, rare earth element geochemistry, and clast compositional data from middle Eocene-Pliocene strata demonstrate the importance of sediment sources located in the retroarc region and along strike within the basin. The Yakutat microplate has recently been reinterpreted to represent buoyant crust that is presently subducting at a shallow angle beneath southern Alaska. Integration of stratigraphic, geochronologic, and thermochronologic data indicate that in the flat-slab region, exhumation initiated ca. 43 Ma and migrated inboard, magmatism ceased at ca. 32 Ma, and deposition in sedimentary basins ended by ca. 23 Ma. Sedimentary basins positioned along the western and northern perimeter of the flat-slab region record enhanced subsidence and sediment delivery from the flat-slab region beginning in late Oligocene and middle Miocene time respectively. The discrete contributions of unique driving forces for lithospheric deformation in western Canada and Alaska have not been quantified in detail, so their relative role in influencing deformation has remained unresolved. Using finite element models, we calculate a continuous strain rate and velocity

  2. Coda Q and its Frequency Dependence in the Eastern Himalayan and Indo-Burman Plate Boundary Systems

    NASA Astrophysics Data System (ADS)

    Mitra, S.; Kumar, A.

    2015-12-01

    We use broadband waveform data for 305 local earthquakes from the Eastern Himalayan and Indo-Burman plate boundary systems, to model the seismic attenuation in NE India. We measure the decay in amplitude of coda waves at discreet frequencies (between 1 and 12Hz) to evaluate the quality factor (Qc) as a function of frequency. We combine these measurements to evaluate the frequency dependence of Qc of the form Qc(f)=Qof η, where Qo is the quality factor at 1Hz and η is the frequency dependence. Computed Qo values range from 80-360 and η ranges from 0.85-1.45. To study the lateral variation in Qo and η, we regionalise the Qc by combining all source-receiver measurements using a back-projection algorithm. For a single back scatter model, the coda waves sample an elliptical area with the epicenter and receiver at the two foci. We parameterize the region using square grids. The algorithm calculates the overlap in area and distributes Qc in the sampled grids using the average Qc as the boundary value. This is done in an iterative manner, by minimising the misfit between the observed and computed Qc within each grid. This process is repeated for all frequencies and η is computed for each grid by combining Qc for all frequencies. Our results reveal strong variation in Qo and η across NE India. The highest Qo are in the Bengal Basin (210-280) and the Indo-Burman subduction zone (300-360). The Shillong Plateau and Mikir Hills have intermediate Qo (~160) and the lowest Qo (~80) is observed in the Naga fold thrust belt. This variation in Qo demarcates the boundary between the continental crust beneath the Shillong Plateau and Mikir Hills and the transitional to oceanic crust beneath the Bengal Basin and Indo-Burman subduction zone. Thick pile of sedimentary strata in the Naga fold thrust belt results in the low Qo. Frequency dependence (η) of Qc across NE India is observed to be very high, with regions of high Qo being associated with relatively higher η.

  3. Oxygen transfer phenomena in 48-well microtiter plates: determination by optical monitoring of sulfite oxidation and verification by real-time measurement during microbial growth.

    PubMed

    Kensy, Frank; Zimmermann, Hartmut F; Knabben, Ingo; Anderlei, Tibor; Trauthwein, Harald; Dingerdissen, Uwe; Büchs, Jochen

    2005-03-20

    Oxygen limitation is one of the most frequent problems associated with the application of shaking bioreactors. The gas-liquid oxygen transfer properties of shaken 48-well microtiter plates (MTPs) were analyzed at different filling volumes, shaking diameters, and shaking frequencies. On the one hand, an optical method based on sulfite oxidation was used as a chemical model system to determine the maximum oxygen transfer capacity (OTR(max)). On the other hand, the Respiration Activity Monitoring System (RAMOS) was applied for online measurement of the oxygen transfer rate (OTR) during growth of the methylotropic yeast Hansenula polymorpha. A proportionality constant between the OTR(max) of the biological system and the OTR(max) of the chemical system were indicated from these data, offering the possibility to transform the whole set of chemical data to biologically relevant conditions. The results exposed "out of phase" shaking conditions at a shaking diameter of 1 mm, which were confirmed by theoretical consideration with the phase number (Ph). At larger shaking diameters (2-50 mm) the oxygen transfer rate in MTPs shaken at high frequencies reached values of up to 0.28 mol/L/h, corresponding to a volumetric mass transfer coefficient (k(L)a) of 1,600 1/h. The specific mass transfer area (a) increases exponentially with the shaking frequency up to values of 2,400 1/m. On the contrary, the mass transfer coefficient (k(L)) is constant at a level of about 0.15 m/h over a wide range of shaking frequencies and shaking diameters. However, at high shaking frequencies, when the complete liquid volume forms a thin film on the cylindric wall of the well, the mass transfer coefficient (k(L)) increases linearly to values of up to 0.76 m/h. Essentially, the present investigation demonstrates that the 48-well plate outperforms the 96-well MTP and shake flasks at widely used operating conditions with respect to oxygen supply. The 48-well plates emerge, therefore, as an excellent

  4. Peridinialean dinoflagellate plate patterns, labels and homologies

    USGS Publications Warehouse

    Edwards, L.E.

    1990-01-01

    Tabulation patterns for peridinialean dinoflagellate thecae and cysts have been traditionally expressed using a plate labelling system described by C.A. Kofoid in the early 1900's. This system can obscure dinoflagellate plate homologies and has not always been strictly applied. The plate-labelling system presented here introduces new series labels but incorporates key features and ideas from the more recently proposed systems of G.L. Eaton and F.J.R. Taylor, as modified by W.R. Evitt. Plate-series recognition begins with the cingulum (C-series) and proceeds from the cingulum toward the apex for the three series of the epitheca/epicyst and proceeds from the cingulum toward the antapex for the two series of the hypotheca/hypocyst. The epithecal/epicystal model consists of eight plates that touch the anterior margin of the cingulum (E-series: plates E1-E7, ES), seven plates toward the apex that touch the E-series plates (M-series: R, M1-M6), and up to seven plates near the apex that do not touch E-series plates (D-series: Dp-Dv). The hypothecal/hypocystal model consists of eight plates that touch the posterior margin of the cingulum (H-series: H1-H6,HR,HS) and three plates toward the antapex (T1-T3). Epithecal/epicystal tabulation patterns come in both 8- and 7- models, corresponding to eight and seven plates, respectively, in the E-series. Hypothecal/hypocystal tabulation patterns also come in both 8- and 7-models, corresponding to eight and seven plates, respectively, in the H-series. By convention, the 7-model epitheca/epicyst has no plates E1 and M1; the 7-model hypotheca/hypocyst has no plate H6. Within an 8-model or 7-model, the system emphasizes plates that are presumed to be homologous by giving them identical labels. I introduce the adjectives "monothigmate", "dithigmate," and "trithigmate" to designate plates touching one, two, and three plates, respectively, of the adjacent series. The term "thigmation" applies to the analysis of plate contacts between

  5. Plated wire memory subsystem

    NASA Technical Reports Server (NTRS)

    Carpenter, K. H.

    1974-01-01

    The design, construction, and test history of a 4096 word by 18 bit random access NDRO Plated Wire Memory for use in conjunction with a spacecraft input/output and central processing unit is reported. A technical and functional description is given along with diagrams illustrating layout and systems operation. Test data is shown on the procedures and results of system level and memory stack testing, and hybrid circuit screening. A comparison of the most significant physical and performance characteristics of the memory unit versus the specified requirements is also included.

  6. Measuring the efficacy of flunixin meglumine and meloxicam for lame sows using a GAITFour pressure mat and an embedded microcomputer-based force plate system.

    PubMed

    Pairis-Garcia, M D; Johnson, A K; Abell, C A; Coetzee, J F; Karriker, L A; Millman, S T; Stalder, K J

    2015-05-01

    Pain associated with lameness on farm is a negative affective state and has a detrimental impact on individual farm animal welfare. Animal pain can be managed utilizing husbandry tools and through pharmacological approaches. Nonsteroidal anti-inflammatory drugs including meloxicam and flunixin meglumine are compounds used in many species for pain management because they are easy to administer, long lasting, and cost-effective. Assessing an animal's biomechanical parameters using such tools as the embedded microcomputer-based force plate system and GAITFour pressure mat gait analysis walkway system provides an objective, sensitive, and precise means to detect animals in lame states. The objectives of this study were to determine the efficacy of meloxicam and flunixin meglumine for pain mitigation in lame sows using the embedded microcomputer-based force plate system and GAITFour pressure mat gait analysis walkway system. Lameness was induced in 24 mature mixed-parity sows using a chemical synovitis model and compared 3 treatments: meloxicam (1.0 mg/kg per os), flunixin meglumine (2.2 mg/kg intramuscular) and sterile saline (intramuscular). Weight distribution (kg) for each foot was collected twice per second for a total of 5 min for each time point using the embedded microcomputer-based force plate system. Stride time, stride length, maximum pressure, activated sensors, and stance time were collected using 3 quality walks (readings) for each time point using the GAITFour pressure mat gait analysis walkway system. Sows administered flunixin meglumine or meloxicam tolerated more weight on their lame leg compared with saline sows (P < 0.005). Sows administered flunixin meglumine or meloxicam had smaller differences in stance time, maximum pressure, and activated sensors between the sound and lame legs compared with saline-treated sows between 37 and 60 h after lameness induction (P < 0.03). In conclusion, flunixin meglumine and meloxicam administration mitigated pain

  7. A simulation study of a dual-plate in-room PET system for dose verification in carbon ion therapy

    NASA Astrophysics Data System (ADS)

    Chen, Ze; Hu, Zheng-Guo; Chen, Jin-Da; Zhang, Xiu-Ling; Guo, Zhong-Yan; Xiao, Guo-Qing; Sun, Zhi-Yu; Huang, Wen-Xue; Wang, Jian-Song

    2014-08-01

    During carbon ion therapy, lots of positron emitters such as 11C, 15O, 10C are generated in irradiated tissues by nuclear reactions, and can be used to track the carbon beam in the tissue by a positron emission tomography (PET) scanner. In this study, an dual-plate in-room PET scanner has been designed and evaluated based on the GATE simulation platform to monitor patient dose in carbon ion therapy. The dual-plate PET is designed to avoid interference with the carbon beamline and with patient positioning. Its performance was compared with that of four-head and full-ring PET scanners. The dual-plate, four-head and full-ring PET scanners consisted of 30, 60, 60 detector modules, respectively, with a 36 cm distance between directly opposite detector modules for dose deposition measurements. Each detector module consisted of a 24×24 array of 2 mm×2 mm×18 mm LYSO pixels coupled to a Hamamatsu H8500 PMT. To estimate the production yield of positron emitters, a 10 cm×15 cm×15 cm cuboid PMMA phantom was irradiated with 172, 200, 250 MeV/u 12C beams. 3D images of the activity distribution measured by the three types of scanner are produced by an iterative reconstruction algorithm. By comparing the longitudinal profile of positron emitters along the carbon beam path, it is indicated that use of the dual-plate PET scanner is feasible for monitoring the dose distribution in carbon ion therapy.

  8. Sputtering and ion plating

    NASA Technical Reports Server (NTRS)

    1972-01-01

    The proceedings of a conference on sputtering and ion plating are presented. Subjects discussed are: (1) concepts and applications of ion plating, (2) sputtering for deposition of solid film lubricants, (3) commercial ion plating equipment, (4) industrial potential for ion plating and sputtering, and (5) fundamentals of RF and DC sputtering.

  9. The contribution of the Volcano Observations Work Package to the implementation of the European Plate Observing System

    NASA Astrophysics Data System (ADS)

    Puglisi, Giuseppe

    2016-04-01

    The overall aim of the implementation phase of European Plate Observing System (EPOS) is to make the integrated platform operational in order to guarantee seamless access to the data provided by the European Solid Earth communities. The Volcano Observations Work Package (WP11) contributes to this objective by implementing a Thematic Core Service (TCS) which is planned to give access to the data and services provided by the European Volcano Observatories (VO) and some Volcanological Research Institutions (VRI; such as university departments, laboratories, etc.). Both types are considered as national research infrastructures (RI) which the TCS will integrate. Currently, monitoring networks on European volcanoes consist of thousands of stations or sites where volcanological parameters are continuously or periodically measured. These sites are equipped with instruments for geophysical (seismic, geodetic, gravimetric, electromagnetic), geochemical (volcanic plumes, fumaroles, groundwater, rivers, soils), environmental observations (e.g. meteorological and air quality parameters), as well as various prototypal monitoring systems (e.g. Doppler radars, ground based SAR). Across Europe several laboratories provide sample characterization (rocks, gases, isotopes, etc.), quasi-continuous analysis of space-borne data (SAR, thermal imagery, SO2 and ash), as well as high-performance computing facilities. All these RIs provide high-quality information (observations) on the current status of European volcanoes and the geodynamic background of the surrounding areas. The implementation of the Volcano Observations TCS will address technical as well as managerial issues, both considering the current heterogeneous state-of-the-art of the volcanological research infrastructures in Europe. Indeed, the current arrangement of individual VO and VRI is considered too fragmented to be considered as a unique distributed infrastructure. Therefore, the main effort in the framework of the EPOS

  10. A new variable angled locking volar plate system for Colles' fracture: outcome study and time-course improvement of objective clinical variables.

    PubMed

    Yasuda, Masataka; Ando, Yoshiyuki

    2009-01-01

    Our purposes were to report the radiographic outcomes and complications of patients with Colles' fracture treated with the Nakashima locking volar plate system (variable angled distal screw locking mechanism) prospectively and to report the results of objective clinical variables such as grip strength and range of motion of the wrist prospectively at up to one year. This study consisted of eight men and 32 women for analysis of radiographic parameters (volar tilt, radial inclination and radial length) and complications. Radiographic parameters were measured pre-operatively, immediately post-operatively and at final follow-up visit. The average age at operation was 60.3 years old. Among them, we selected 25 cases (6 men and 19 women) whom we followed up at six weeks, three months, six months and one year post-operatively. The average age at operation in this group was 62 years old. We measured objective clinical variables (grip strength, forearm rotation, wrist extension/flexion) at each visit. Except for volar tilt, radiographic parameters revealed no significant changes between immediately post-operative radiographs and radiographs at final follow-up visit. Complications included loss of reduction in two cases. Objective clinical variables other than pronation measurement showed significant increase at each visit up to one year post-operatively. Satisfactory clinical and radiographic results were obtained by using this system. The variable angled distal fragment plating system appears to be a reliable construct for rigid fixation of Colles' fractures; however, technical errors can occur, as with other fixation systems. We demonstrated that the follow-up of Colles' fracture treated by our volar locking plate less than one year post-operative may be insufficient.

  11. Development and testing of a multiwell plates absorbance reader for clinical analysis using inexpensive webcam

    NASA Astrophysics Data System (ADS)

    Castillo, Jimmy; Gutierrez, Hector; Vitta, Yosmery; Martinez, Mauro; Fernandez, Alberto

    2007-09-01

    Biochemical analysis and clinical tests like glucose, hemoglobin, cholesterol, iron, etc. are crucial for early illness diagnosis like diabetes, anemia and coronary deceases. These tests usually are done in state of the art instruments in well equipped laboratories in health centers. In some cases, these instruments are not portable, so they are not recommended for clinical field studies in remote areas. The present work shows a portable low-cost prototype of multi-well plates reader designed for clinical analysis. A Light Emission Diodes (LEDs) array is used as excitation source and an inexpensive webcam as detector. The light source illuminates the 96 well plates and the webcam take the image with 640x480 pixels. The data is acquired and processed by using a portable computer. 96 samples can be read including blanks and calibration standards simultaneously. Light absorption data are processed using a MatLab software designed in our laboratory to obtain calibration curves, standards lectures and samples concentration. The system was evaluated using different analytes series solutions: Neutral Red, Cooper (II) Ammonia Complex and Methyl Orange. The results shows that it is possible to measure few micro liters of solutions with adequate exactitude and precision of less than 3%. As possible analytical clinical application, iron determination was performed using Fe(III) Thiocyanate complex. This method is usually applied in serum samples analysis. The sensibility achieved with the proposed instrumentation configurations allows the analysis of iron in serum samples in the references values normal range (0.75 - 1.5 mg/L) in human.

  12. Measurement of spatial distribution of neutrons and gamma rays for BNCT using multi-imaging plate system.

    PubMed

    Tanaka, Kenichi; Sakurai, Yoshinori; Tanaka, Hiroki; Kajimoto, Tsuyoshi; Takata, Takushi; Takada, Jun; Endo, Satoru

    2015-12-01

    Quality assurance of the spatial distributions of neutrons and gamma rays was tried using imaging plates (IPs) and converters to enhance the beam components in the epithermal neutron mode of the Kyoto University Reactor. The converters used were 4mm thick epoxy resin with B4C at 6.85 weight-percent (wt%) (10)B for epithermal neutrons, and 3mm thick carbon for gamma rays. Results suggested that the IP signal does not need a sensitivity correction regardless of the incident radiation that produces it.

  13. Position-sensitive detector system OBI for High Resolution X-Ray Powder Diffraction using on-site readable image plates

    NASA Astrophysics Data System (ADS)

    Knapp, M.; Joco, V.; Baehtz, C.; Brecht, H. H.; Berghaeuser, A.; Ehrenberg, H.; von Seggern, H.; Fuess, H.

    2004-04-01

    A one-dimensional detector system has been developed using image plates. The detector is working in transmission mode or Debye-Scherrer geometry and is on-site readable which reduces the effort for calibration. It covers a wide angular range up to 110° and shows narrow reflection half-widths depending on the capillary diameter. The acquisition time is in the range of minutes and the data quality allows for reliable Rietveld refinement of complicated structures, even in multi-phase samples. The detector opens a wide field of new applications in kinetics and temperature resolved measurements.

  14. The behavior of organic components in copper recovery from electroless plating bath effluents using 3D electrode systems.

    PubMed

    Orhan, Gökhan; Gürmen, Sebahattin; Timur, Servet

    2004-08-30

    An electrochemical method was applied for the recovery of copper both from the spent solutions and from the rinse waters of electroless copper plating baths, containing copper sulfate, formaldehyde, quadrol, and NaOH. Experiments were conducted in a rotating packed cell (Rollschichtzelle) to investigate the effects of current density, electrolyte composition, temperature, and pH on the copper recovery. All the copper (final CCu=0.1 ppm) was recovered from the waste and rinse waters of chemical copper plating plants with 70% current efficiency by the electrochemical treatment in a rotating packed cell at 130 A/m2 current density, room temperature, with 5mm diameter cathode granules, with the presence of formaldehyde, and with a specific energy consumption of 3.2-3.5 kW h/kg Cu. On the other hand, final copper concentrations of 5 ppm were reached with 62% current efficiency and 5.5-5.8 kW h/kg Cu specific energy consumption, with electrolytes containing no formaldehyde.

  15. Effect of inherited structures on strike-slip plate boundaries: insight from analogue modelling of the central Levant Fracture System, Lebanon

    NASA Astrophysics Data System (ADS)

    Ghalayini, Ramadan; Daniel, Jean-Marc; Homberg, Catherine; Nader, Fadi

    2015-04-01

    Analogue sandbox modeling is a tool to simulate deformation style and structural evolution of sedimentary basins. The initial goal is to test what is the effect of inherited and crustal structures on the propagation, evolution, and final geometry of major strike-slip faults at the boundary between two tectonic plates. For this purpose, we have undertaken a series of analogue models to validate and reproduce the structures of the Levant Fracture System, a major NNE-SSW sinistral strike-slip fault forming the boundary between the Arabian and African plates. Onshore observations and recent high quality 3D seismic data in the Levant Basin offshore Lebanon demonstrated that Mesozoic ENE striking normal faults were reactivated into dextral strike-slip faults during the Late Miocene till present day activity of the plate boundary which shows a major restraining bend in Lebanon with a ~ 30°clockwise rotation in its trend. Experimental parameters consisted of a silicone layer at the base simulating the ductile crust, overlain by intercalated quartz sand and glass sand layers. Pre-existing structures were simulated by creating a graben in the silicone below the sand at an oblique (>60°) angle to the main throughgoing strike-slip fault. The latter contains a small stepover at depth to create transpression during sinistral strike-slip movement and consequently result in mountain building similarly to modern day Lebanon. Strike-slip movement and compression were regulated by steady-speed computer-controlled engines and the model was scanned using a CT-scanner continuously while deforming to have a final 4D model of the system. Results showed that existing normal faults were reactivated into dextral strike-slip faults as the sinistral movement between the two plates accumulated. Notably, the resulting restraining bend is asymmetric and segmented into two different compartments with differing geometries. One compartment shows a box fold anticline, while the second shows an

  16. Silencing using flexible plate in a duct

    NASA Astrophysics Data System (ADS)

    Ramamoorthy, Sripriya; Grosh, Karl; Nawar, Tony G.

    2002-11-01

    A flexible plate interacting with air in a duct can provide passive means for low frequency broadband transmission loss. The sensitivity of the system to various parameters including unintentionally applied tension, effect of external fluid loading, lateral plate cross modes, structural and acoustic boundary condition are analyzed through experimental measurements and theoretical predictions. In order to avoid breakout noise, a backing cavity can be introduced below the plate. This introduces differences in filtering characteristics. Compared to plate in a single duct, the two-duct system will have higher plate resonance frequencies due to cavity loading on the plate. Means to achieve low frequency broadband transmission loss using two-duct silencers will be discussed. Significance of three dimensionality of the problem will be brought out by comparing the results of three-dimensional finite-element analysis with experimental data. Successful designs and experiments for low frequency attenuation will be presented. [Work supported by NSF and ONR.

  17. Extending cycle life of lead-acid batteries: a new separation system allows the application of pressure on the plate group

    NASA Astrophysics Data System (ADS)

    Perrin, M.; Döring, H.; Ihmels, K.; Weiss, A.; Vogel, E.; Wagner, R.

    Since 1983, it has been claimed that pressure applied on a lead-acid battery increases its cycle life. But until now, the use of pressure in production batteries was limited by the mechanical properties of the conventional separation systems (absorptive glass mat (AGM), and gel) which cannot withstand mechanical pressure. In 1997, Daramic developed the new acid jellying separator (AJS) with the aim of combining the advantages of both conventional separation systems and to allow the application of lasting plate group pressure. The new separation system was evaluated and much information was gained on the effect of pressure in a lead-acid battery, e.g. on the evolution of the mechanical pressure during one cycle and during cycle life.

  18. A high-throughput multicomponent screening method for diuretics, masking agents, central nervous system (CNS) stimulants and opiates in human urine by UPLC-MS/MS.

    PubMed

    Thörngren, John-Olof; Ostervall, Fredrik; Garle, Mats

    2008-07-01

    A simple and rapid multicomponent screening method of 130 substances for direct injections of urine samples has been developed. The fully automated method based on ultra-performance liquid chromatography (UPLC) and tandem mass spectrometry (MS/MS) is used for three different classes of doping agents: diuretics, central nervous system stimulants (CNS stimulants) and opiates. The samples are diluted with buffer containing internal standards (IS) by a pipetting robot system into 96-well plates. Samples are injected on a reversed phase sub 2-microm particle column connected to a fast polarity switching and rapid scanning tandem mass spectrometer with an electrospray interface. The software used to evaluate the results produced reports containing a small-sized window for each component and a data table list with flags to indicate any adverse analytical findings in the sample. The report can also be processed automatically using an application software, which interpret the data and indicate if there is a suspicious sample. One 96-well plate can be analyzed within 16 h.

  19. Earth's Decelerating Tectonic Plates

    SciTech Connect

    Forte, A M; Moucha, R; Rowley, D B; Quere, S; Mitrovica, J X; Simmons, N A; Grand, S P

    2008-08-22

    Space geodetic and oceanic magnetic anomaly constraints on tectonic plate motions are employed to determine a new global map of present-day rates of change of plate velocities. This map shows that Earth's largest plate, the Pacific, is presently decelerating along with several other plates in the Pacific and Indo-Atlantic hemispheres. These plate decelerations contribute to an overall, globally averaged slowdown in tectonic plate speeds. The map of plate decelerations provides new and unique constraints on the dynamics of time-dependent convection in Earth's mantle. We employ a recently developed convection model constrained by seismic, geodynamic and mineral physics data to show that time-dependent changes in mantle buoyancy forces can explain the deceleration of the major plates in the Pacific and Indo-Atlantic hemispheres.

  20. Accelerated plate tectonics.

    PubMed

    Anderson, D L

    1975-03-21

    The concept of a stressed elastic lithospheric plate riding on a viscous asthenosphere is used to calculate the recurrence interval of great earthquakes at convergent plate boundaries, the separation of decoupling and lithospheric earthquakes, and the migration pattern of large earthquakes along an arc. It is proposed that plate motions accelerate after great decoupling earthquakes and that most of the observed plate motions occur during short periods of time, separated by periods of relative quiescence.

  1. Side-by-side comparisons of evacuated compound parabolic concentrator and flat plate solar collector systems at temperatures of 90 to 100C

    NASA Astrophysics Data System (ADS)

    Allen, J. W.; Schertz, W. W.; Wantroba, A. S.

    1987-03-01

    This collector system study is an extension of a previous system study in which Argonne National Laboratory (ANL) compared the performance of three solar energy systems operated side by side for over a year. In the present system study, four solar energy systems were operated side by side for part of a year. Two of the collector systems used commercially available compound parabolic concentrator (CPC) collectors, one used a commercially available flat plate collector, and one used an experimental CPC collector built by The University of Chicago. The collectors were mounted in fixed positions; they did not track the Sun, and their tilt angles were not seasonally adjusted. All of the collector arrays faced south and were tilted at 42 deg with respect to the horizon (to match the 42 deg N latitude at ANL). All four collector systems started each day with their storage temperatures at 90 C. During the day, each system was operated by its own solar controller. At the end of the day, the tanks were mixed and the temperature changes in the tanks were measured. The change in storage energy was calculated from the temperature change, the heat capacity of the storage system, and the pump energy.

  2. Obliquity along plate boundaries

    NASA Astrophysics Data System (ADS)

    Philippon, Mélody; Corti, Giacomo

    2016-12-01

    Most of the plate boundaries are activated obliquely with respect to the direction of far field stresses, as roughly only 8% of the plate boundaries total length shows a very low obliquity (ranging from 0 to 10°, sub-orthogonal to the plate displacement). The obliquity along plate boundaries is controlled by (i) lateral rheological variations within the lithosphere and (ii) consistency with the global plate circuit. Indeed, plate tectonics and magmatism drive rheological changes within the lithosphere and consequently influence strain localization. Geodynamical evolution controls large-scale mantle convection and plate formation, consumption, and re-organization, thus triggering plate kinematics variations, and the adjustment and re-orientation of far field stresses. These geological processes may thus result in plate boundaries that are not perpendicular but oblique to the direction of far field stresses. This paper reviews the global patterns of obliquity along plate boundaries. Using GPlate, we provide a statistical analysis of present-day obliquity along plate boundaries. Within this framework, by comparing natural examples and geological models, we discuss deformation patterns and kinematics recorded along oblique plate boundaries.

  3. Plating Tank Control Software

    SciTech Connect

    Krafcik, John

    1998-03-01

    The Plating Tank Control Software is a graphical user interface that controls and records plating process conditions for plating in high aspect ratio channels that require use of low current and long times. The software is written for a Pentium II PC with an 8 channel data acquisition card, and the necessary shunt resistors for measuring currents in the millampere range.

  4. Rotatable shear plate interferometer

    DOEpatents

    Duffus, Richard C.

    1988-01-01

    A rotatable shear plate interferometer comprises a transparent shear plate mounted obliquely in a tubular supporting member at 45.degree. with respect to its horizontal center axis. This tubular supporting member is supported rotatably around its center axis and a collimated laser beam is made incident on the shear plate along this center axis such that defocus in different directions can be easily measured.

  5. Characterization of antibody binding to cell surface antigens using a plasma membrane-bound plate assay.

    PubMed

    Vater, C A; Reid, K; Bartle, L M; Goldmacher, V S

    1995-01-01

    A procedure has been developed for measuring antibody binding to cell surface antigens using an immobilized plasma membrane fraction. In this method, isolated plasma membranes are dried onto wells of a 96-well microtiter plate and incubated with antibodies that recognize a cell surface protein. Bound antibody is detected indirectly using an enzyme-linked or fluorescently tagged second antibody. Alternatively, the primary antibody itself can be labeled and its binding can be detected directly. The assay is simple and fast and provides several advantages over whole cell binding assays currently in widespread use.

  6. Beyond plate tectonics - Looking at plate deformation with space geodesy

    NASA Technical Reports Server (NTRS)

    Jordan, Thomas H.; Minster, J. Bernard

    1988-01-01

    The requirements that must be met by space-geodetic systems in order to constrain the horizontal secular motions associated with the geological deformation of the earth's surface are explored. It is suggested that in order to improve existing plate-motion models, the tangential components of relative velocities on interplate baselines must be resolved to an accuracy of less than 3 mm/yr. Results indicate that measuring the velocities between crustal blocks to + or - 5 mm/yr on 100-km to 1000-km scales can produce geologically significant constraints on the integrated deformation rates across continental plate-boundary zones such as the western United States.

  7. Production of 6cm x 6cm Micro-channel Plate Based Picosecond Photodetectors with the Argonne Small Tile Processing System (STPS)

    NASA Astrophysics Data System (ADS)

    Xia, Lei; Byrum, Karen; Demarteau, Marcel; Wagner, Robert; Walters, Dean; Wang, Jingbo; Xie, Junqi; Zhao, Huyue

    2015-04-01

    Microchannel plate (MCP) based photodetectors feature fast timing, good position resolution and compact form factor. However, traditional MCP photodetectors suffer from limited charge lifetime and high cost. The LAPPD collaboration, over the years, developed Atomic Layer Deposition (ALD) coated new generation MCP's and low cost glass packaging technology. Recently, the Argonne group commissioned its small form factor tile processing system and produced the first fully processed sealed photodetectors with glass packaging, using the ALD coated MCP's. We report the design, construction and commissioning of the system, and production of the first devices. Supported by the U. S. Department of Energy, Office of Science, Office of Basic Energy Sciences and Office of High Energy Physics under Contract DE-AC02-06CH11357.

  8. Malachite green photosensitive plates.

    PubMed

    Solano, C

    1989-08-15

    An experimental study of the behavior of malachite green sensitized plates was carried out. The transmittance variation of the irradiated plates was taken as a parameter. It has been observed that photoreduction in the malachite green plates is present only when ammonium dichromate is added to the plates. The introduction of external electron donors does not improve the photochemical reaction. It has been determined that malachite green molecules form a weak complex with the dichromate molecules and this complex can only be destroyed photochemically. This effect can explain the limited response of the malachite green dichromated plates.

  9. [The use of blade plate and dynamic screw plate osteosynthesis].

    PubMed

    Oestern, H J; Gänsslen, A

    2010-02-01

    Osteosynthesis in fracture treatment and in some reconstructive procedures with blade plates or dynamic screw systems was the standard procedure for several decades. In this review, the current options and concepts using blade plate osteosynthesis, stabilization of proximal and distal femur fractures and reconstructive procedures with the dynamic hip screw or the dynamic condylar blade are discussed. On the basis of a literature review, the present indications, results and region-specific complications are reported and discussed.Blade plates are used mainly in the context of reconstructive procedures, as well as in the treatment of pseudoarthroses. The Pauwel procedure in femoral neck non-unions is one of the best known indications. In contrast, the dynamic hip screw is the gold standard for stabilization of femoral neck and most pertrochanteric fractures, whereas the dynamic condylar screw is still an alternative to internal fixators for proximal and distal femoral fracture fixations.

  10. A comparison of surface wave tomography in the Atlantic Ocean with the plate model: mismatches and implications for the lithosphere-asthenosphere system

    NASA Astrophysics Data System (ADS)

    Fishwick, S.; Crosby, A. G.

    2009-12-01

    of the lithoshere-asthenosphere system. Although previous studies of the Pacific have shown an agreement between seismic data and thermal models involving small scale convection, the seismic results for the Atlantic suggest that the oceanic lithosphere continues to cool even beneath the older ocean, which is at odds with a literal interpretation of the plate models that fit subsidence and heat flow data. One possible explanation for the discrepancy is that the oceanic lithosphere can continue to cool, but subside as though it has a fixed thickness of 95km, if the vertical stresses are attenuated by a low-viscosity layer. Further quantitative studies will assess if this mechanism is viable, and should place valuable constraints on the viscosity structure of the asthenosphere. More immediately, our results suggest that we should be cautious in assuming we have an accurate knowledge of the temperature structure beneath the oceans. Their thermal evolution remains, in many respects, an unsolved problem.

  11. Multicolor printing plate joining

    NASA Technical Reports Server (NTRS)

    Waters, W. J. (Inventor)

    1984-01-01

    An upper plate having ink flow channels and a lower plate having a multicolored pattern are joined. The joining is accomplished without clogging any ink flow paths. A pattern having different colored parts and apertures is formed in a lower plate. Ink flow channels each having respective ink input ports are formed in an upper plate. The ink flow channels are coated with solder mask and the bottom of the upper plate is then coated with solder. The upper and lower plates are pressed together at from 2 to 5 psi and heated to a temperature of from 295 F to 750 F or enough to melt the solder. After the plates have cooled and the pressure is released, the solder mask is removed from the interior passageways by means of a liquid solvent.

  12. MHD Heat Transfer in Two-Layered Flow of Conducting Fluids through a Channel Bounded by Two Parallel Porous Plates in a Rotating System

    NASA Astrophysics Data System (ADS)

    Linga Raju, T.; Neela Rao, B.

    2016-08-01

    The paper aims to analyze the heat transfer aspects of a two-layered fluid flow in a horizontal channel under the action of an applied magnetic and electric fields, when the whole system is rotated about an axis perpendicular to the flow. The flow is driven by a common constant pressure gradient in the channel bounded by two parallel porous insulating plates, one being stationary and the other one oscillatory. The fluids in the two regions are considered electrically conducting, and are assumed to be incompressible with variable properties, namely, different densities, viscosities, thermal and electrical conductivities. The transport properties of the two fluids are taken to be constant and the bounding plates are maintained at constant and equal temperature. The governing partial differential equations are then reduced to the ordinary linear differential equations by using a two-term series. The temperature distributions in both fluid regions of the channel are derived analytically. The results are presented graphically to discuss the effect on the heat transfer characteristics and their dependence on the governing parameters, i.e., the Hartmann number, Taylor number, porous parameter, and ratios of the viscosities, heights, electrical and thermal conductivities. It is observed that, as the Coriolis forces become stronger, i.e., as the Taylor number increases, the temperature decreases in the two fluid regions. It is also seen that an increase in porous parameter diminishes the temperature distribution in both the regions.

  13. Capacitive carbon and electrochemical lead electrode systems at the negative plates of lead-acid batteries and elementary processes on cycling

    NASA Astrophysics Data System (ADS)

    Pavlov, D.; Nikolov, P.

    2013-11-01

    Batteries in hybrid electric vehicles operate in High-Rate Partial-State-of-Charge (HRPSoC) cycling duty. To make lead-acid batteries suitable for this duty, carbon is added to the negative active material. As a result of this technological change, two electrical systems form at the negative plates: (a) a capacitive carbon system comprising high-rate charging and discharging of the electric double layer; low Ah capacity, and (b) a lead electrochemical system, comprising oxidation of Pb to PbSO4 during discharge and vice versa during charge; this system is slow to accept charge, but has high Ah capacity. Through cycling lead-acid cells under HRPSoC conditions with short current pulses of various durations we have established that the processes involved in the capacitive system proceed highly reversibly and complete hundreds of thousands HRPSoC cycles. The number of cycles achieved by the electrochemical system is limited to tens of thousands and lead to progressive sulfation. Carbon added to the negative active material changes the latter's structure. The specific surface of NAM increases and the median pore radius decreases. Some carbon additives may reduce the radius of the pores in NAM to membrane sizes, which may change the chemistry of the electrochemical system.

  14. Monitoring the production of inclusion bodies during fermentation and enzyme-linked immunosorbent assay analysis of intact inclusion bodies using cryogel minicolumn plates.

    PubMed

    Ahlqvist, Josefin; Dainiak, Maria B; Kumar, Ashok; Hörnsten, E Gunnar; Galaev, Igor Yu; Mattiasson, Bo

    2006-07-15

    A novel minicolumn chromatographic method to monitor the production of inclusion bodies during fermentation and an enzyme-linked immunosorbent assay (ELISA) system allowing direct analysis of the particles with surface-displayed antigens are described. A 33-kDa protein containing 306 amino acids with three sulfur bridges produced as inclusion bodies was labeled with polyclonal antibodies against 15 amino acid (anti-A15) and 17 amino acid (anti-B17) residues at the N- and C-terminal ends of the protein, respectively. Labeled particles were bound to macroporous monolithic protein A-cryogel adsorbents inserted into the open-ended wells of a 96-well plate (referred to as protein A-cryogel minicolumn plate). The concept behind this application is that the binding degree of inclusion bodies from lysed fermentation broth to the cryogel minicolumns increases with an increase in their concentration during fermentation. The technique allowed us to monitor the increase in the production levels of the inclusion bodies as the fermentation process progressed. The system also has a built-in quality parameter to ensure that the target protein has been fully expressed. Alternatively, inclusion bodies immobilized on phenyl-cryogel minicolumn plate were used in indirect ELISA based on anti-A15 and anti-B17 antibodies against terminal amino acid residues displayed on the surface of inclusion bodies. Drainage-protected properties of the cryogel minicolumns allow performance of successive reactions with tested immunoglobulin G (IgG) samples and enzyme-conjugated secondary IgG and of enzymatic reaction within the adsorbent.

  15. 49 CFR 236.779 - Plate, top.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Plate, top. 236.779 Section 236.779 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF... OF SIGNAL AND TRAIN CONTROL SYSTEMS, DEVICES, AND APPLIANCES Definitions § 236.779 Plate, top....

  16. 49 CFR 236.779 - Plate, top.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Plate, top. 236.779 Section 236.779 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF... OF SIGNAL AND TRAIN CONTROL SYSTEMS, DEVICES, AND APPLIANCES Definitions § 236.779 Plate, top....

  17. Evacuated flat-plate solar collectors

    SciTech Connect

    Whittemore, P.G.

    1981-09-15

    A structural support system is disclosed for use in an evacuated, flat-plate, solar collector to eliminate the problem of stress fractures in a glass cover plate. Nonlinearly spaced supports are used within the collector to dampen vibrations in the glass cover and to prevent overdeflection before buckling or stress fractures occur.

  18. Optics for People Stuck in Traffic: License Plates.

    ERIC Educational Resources Information Center

    Chagnon, Paul

    1995-01-01

    Explains the theory behind the working of Scotchlite, a retrodirective material used for coating automotive license plates, and the Ensure Imaging System that allows law enforcement officers to verify the authenticity of the plate. (JRH)

  19. Combining Facial Recognition, Automatic License Plate Readers and Closed Circuit Television to Create an Interstate Identification System for Wanted Subjects

    DTIC Science & Technology

    2015-12-01

    and CCTV systems has been created. The intent of this thesis is to serve as catalyst for a new security system designed to locate, identify, and...serve as catalyst for a new security system designed to locate, identify, and apprehend known terrorist watch list suspects and other wanted persons... catalyst for the formation of a new security system designed to locate, identify and apprehend known terrorist watch list suspects and other wanted

  20. A sensitivity analysis of central flat-plate photovoltaic systems and implications for national photovoltaics program planning

    NASA Technical Reports Server (NTRS)

    Crosetti, M. R.

    1985-01-01

    The sensitivity of the National Photovoltaic Research Program goals to changes in individual photovoltaic system parameters is explored. Using the relationship between lifetime cost and system performance parameters, tests were made to see how overall photovoltaic system energy costs are affected by changes in the goals set for module cost and efficiency, system component costs and efficiencies, operation and maintenance costs, and indirect costs. The results are presented in tables and figures for easy reference.

  1. Comparison of Petrifilm Staph Express Count System with the bacteriological analytical manual direct-plating method for enumeration of Staphylococcus aureus in artificially contaminated hard cheese.

    PubMed

    Fedio, Willis M; Wendakoon, Chitra N; Zapata, Ruben; Carrillo, Christina; Browning, Paul

    2008-01-01

    The 3M Petrifilm Staph Express Count System was compared with the U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) direct-plate count method for the enumeration of Staphylococcus aureus in 6 types of artificially contaminated hard cheese (Asiago, Cheddar, Gruyère, Parmesan, Romano, and Swiss). Five different samples of each cheese type were inoculated with S. aureus (ATCC 25923) to achieve low, medium, and high inoculum levels. S. aureus was enumerated by the Petrifilm and BAM methods, and the results were compared. Multivariate analysis of variance revealed no significant differences (P<0.05) between the 2 methods. The Petrifilm method compared favorably with the BAM procedure. The rapid method was more convenient to use, considerably faster, and less expensive to perform than the BAM method.

  2. GOLD PLATING PROCESS

    DOEpatents

    Seegmiller, R.

    1957-08-01

    An improved bath is reported for plating gold on other metals. The composition of the plating bath is as follows: Gold cyanide from about 15 to about 50 grams, potassium cyanide from about 70 to about 125 grams, and sulfonated castor oil from about 0.1 to about 10 cc. The gold plate produced from this bath is smooth, semi-hard, and nonporous.

  3. Plating methods, a survey

    NASA Technical Reports Server (NTRS)

    Berkowitz, J. B.; Emerson, N. H.

    1972-01-01

    Results are presented of a comprehensive search of the literature available, much of which has been generated by the research centers of NASA and its contractors, on plating and coating methods and techniques. Methods covered included: (1) electroplating from aqueous solutions; (2) electroplating from nonaqueous solutions; (3) electroplating from fused-salt baths; (4) electroforming; (5) electroless plating, immersion plating, and mirroring; (6) electroplating from gaseous plasmas; and (7) anodized films and conversion coatings.

  4. Shock wave absorber having apertured plate

    DOEpatents

    Shin, Y.W.; Wiedermann, A.H.; Ockert, C.E.

    1983-08-26

    The shock or energy absorber disclosed herein utilizes an apertured plate maintained under the normal level of liquid flowing in a piping system and disposed between the normal liquid flow path and a cavity pressurized with a compressible gas. The degree of openness (or porosity) of the plate is between 0.01 and 0.60. The energy level of a shock wave travelling down the piping system thus is dissipated by some of the liquid being jetted through the apertured plate toward the cavity. The cavity is large compared to the quantity of liquid jetted through the apertured plate, so there is little change in its volume. The porosity of the apertured plate influences the percentage of energy absorbed.

  5. Shock wave absorber having apertured plate

    DOEpatents

    Shin, Yong W.; Wiedermann, Arne H.; Ockert, Carl E.

    1985-01-01

    The shock or energy absorber disclosed herein utilizes an apertured plate maintained under the normal level of liquid flowing in a piping system and disposed between the normal liquid flow path and a cavity pressurized with a compressible gas. The degree of openness (or porosity) of the plate is between 0.01 and 0.60. The energy level of a shock wave travelling down the piping system thus is dissipated by some of the liquid being jetted through the apertured plate toward the cavity. The cavity is large compared to the quantity of liquid jetted through the apertured plate, so there is little change in its volume. The porosity of the apertured plate influences the percentage of energy absorbed.

  6. PLATES WITH OXIDE INSERTS

    DOEpatents

    West, J.M.; Schumar, J.F.

    1958-06-10

    Planar-type fuel assemblies for nuclear reactors are described, particularly those comprising fuel in the oxide form such as thoria and urania. The fuel assembly consists of a plurality of parallel spaced fuel plate mennbers having their longitudinal side edges attached to two parallel supporting side plates, thereby providing coolant flow channels between the opposite faces of adjacent fuel plates. The fuel plates are comprised of a plurality of longitudinally extending tubular sections connected by web portions, the tubular sections being filled with a plurality of pellets of the fuel material and the pellets being thermally bonded to the inside of the tubular section by lead.

  7. CALUTRON FACE PLATE

    DOEpatents

    Brobeck, W.M.

    1959-08-25

    The construction of a removable cover plate for a calutron tank is described. The plate is fabricated of a rectangular frame member to which is welded a bowed or dished plate of thin steel, reinforced with transverse stiffening ribs. When the tank is placed between the poles of a magnet, the plate may be pivoted away from the tank and magnet and is adapted to support the ion separation mechanism secured to its inner side as well as the vacuum load within the tank.

  8. Exhumation History of an Oblique Plate Boundary: Investigating Kaikoura Mountain-building within the Marlborough Fault System, NE South Island New Zealand

    NASA Astrophysics Data System (ADS)

    Collett, C.; Duvall, A. R.; Flowers, R. M.; Tucker, G. E.

    2015-12-01

    The Kaikoura Mountains stand high as topographic anomalies in the oblique Pacific-Australian plate boundary zone known as the Marlborough Fault System (MFS), NE South Island New Zealand. The base of both the Inland and Seaward Kaikoura Ranges are bound on the SE by major, steeply NW-dipping, right lateral, active strike-slips (Clarence and Hope faults of the MFS, respectively). Previous geologic mapping, observations of predominantly horizontal fault slip at the surface from GPS and offset Quaternary deposits, and uplift of marine terraces, provide evidence for shortening and mountain-building via distributed deformation off of the main MFS strike-slip faults. However, quantitative estimates of the magnitude and spatial patterns of exhumation and of the timing of mountain-building in the Kaikouras are needed to understand more fully the nature of oblique deformation in the MFS. We present new apatite and zircon (U-Th)/He ages from opposite sides of the Hope and Clarence faults, spanning over 2 km of relief within the Kaikoura Mountains to identify spatial and temporal changes in exhumation rates in relation to the adjacent faults. Young (~3 Ma) apatite He ages and rapid (potentially > 1 mm/yr) exhumation rates from opposite sides of the faults are consistent with previously mentioned evidence of recent, regional, distributed deformation off of the main MFS faults. Moreover, early Miocene zircon He ages imply that parts of this region experienced an earlier phase of fault-related exhumation. Large changes in zircon He ages across the faults from ~20 Ma to > 100 Ma support hypotheses that portions of the Marlborough Faults may be re-activated, early Miocene thrusts. The zircon data are also consistent with the hypothesis of an early Miocene initiation of the oblique Pacific-Australian plate boundary in this region. Evidence for this comes from a change in sedimentation during this time from fine marine sediments to coarse, terrigenous conglomerates. Observing more

  9. Partial Melt Systems in Plate-Driven Corner Flow: Evaluating the Formation of Porosity Bands as a Mechanism for Magma Focusing at Mid-Ocean Ridges

    NASA Astrophysics Data System (ADS)

    Gebhardt, D.; Butler, S. L.

    2015-12-01

    The imposition of an external shear on a system of partial melt will result in compaction of the solid matrix and concentration of the interstitial liquid melt leading to the formation of regions of contrasting high and low porosity. In experiments, direct and torsional shear geometries have demonstrated that these regions of varying porosity form in bands orientated at low angles relative to the shear plane. A variety of numerical models have been employed to recreate these experimental results. Simple shear, pure shear and torsional shear geometries have been used in both linear and nonlinear numerical settings to model the formation of the porosity bands. In this contribution the numerical models utilize a shear geometry derived from the velocity field of the plate-driven corner flow of a mid-ocean ridge. Motivation for using the velocity field of a mid-ocean ridge comes from evidence that suggests the existence of lateral melt channeling from either side of the ridge axis. Imposing the shear from a mid-ocean ridge corner flow allows for the evaluation of the resulting porosity bands in terms of suitability for channeling melt laterally toward the ridge axis. This is done using both slow and fast spreading ridge geometries. The degree of similarity between previous numerical and experimental results has been found to be greatly influenced by the imposed viscosity law of the solid matrix phase. In order to keep this in mind, the numerical models in this contribution use three different matrix viscosity laws: strain-rate independent, strain-rate dependent and anisotropic. Of these rheologies, strain-rate independence results in the poorest orientation for channeling melt directly to the ridge axis. The strain-rate dependent and anisotropic viscosities present more favorable direct-channeling orientations for the fastest growing porosity bands, but in both cases the background flow will rotate bands to less ideal orientations over time. However, these less

  10. Continuous deflation and plate spreading at the Askja volcanic system, Iceland: Constrains on deformation processes from finite element models using temperature-dependent non-linear rheology

    NASA Astrophysics Data System (ADS)

    Tariqul Islam, Md.; Sturkell, Erik; Sigmundsson, Freysteinn; Drouin, Vincent Jean Paul B.; Ófeigsson, Benedikt G.

    2014-05-01

    Iceland is located on the mid Atlantic ridge, where the spreading rate is nearly 2 cm/yr. The high rate of magmatism in Iceland is caused by the interaction between the Iceland hotspot and the divergent mid-Atlantic plate boundary. Iceland hosts about 35 volcanoes or volcanic systems that are active. Most of these are aliened along the plate boundary. The best studied magma chamber of central volcanoes (e.g., Askja, Krafla, Grimsvötn, Katla) have verified (suggested) a shallow magma chamber (< 5 km), which has been model successfully with a Mogi source, using elastic and/or elastic-viscoelastic half-space. Maxwell and Newtonian viscosity is mainly considered for viscoelastic half-space. Therefore, rheology may be oversimplified. Our attempt is to study deformation of the Askja volcano together with plate spreading in Iceland using temperature-dependent non-linear rheology. It offers continuous variation of rheology, laterally and vertically from rift axis and surface. To implement it, we consider thermo-mechanic coupling models where rheology follows dislocation flow in dry condition based on a temperature distribution. Continuous deflation of the Askja volcanic system is associated with solidification of magma in the magma chamber and post eruption relaxation. A long time series of levelling data show its subsidence trend to exponentially. In our preliminary models, a magma chamber at 2.8 km depth with 0.5 km radius is introduced at the ridge axis as a Mogi source. Simultaneously far field of rift axis stretching by 18.4 mm/yr (measured during 2007 to 20013) is applied to reproduce plate spreading. Predicted surface deformation caused of combined effect of tectonic-volcanic activities is evaluated with GPS during 2003-2009 and RADARSAT InSAR data during 2000 to 2010. During 2003-2009, data from the GPS site OLAF (close to the centre of subsidence) shows average rate of subsidence 19±1 mm/yr relative to the ITRF2005 reference frame. The MASK (Mid ASKJA) site is

  11. Far field effects of Alpine plate tectonism in the Iberian microplate recorded by fault-related denudation in the Spanish Central System

    NASA Astrophysics Data System (ADS)

    de Bruijne, C. H.; Andriessen, P. A. M.

    2002-05-01

    Apatite fission track analysis was performed on 56 samples from central Spain to unravel the far field effects of the Alpine plate tectonic history of Iberia. The modelled thermal histories reveal complex cooling in the Cenozoic, indicative of intermittent denudation. Accelerated cooling events occurred across the Spanish Central System (SCS) from the Middle Eocene to Recent. These accelerated cooling events resulted in up to 2.8±0.9 km of denudation in the western Sierra de Gredos and 3.6±1.0 km in the central and eastern Gredos (assuming a paleogeothermal gradient of 28±5 °C and a surface temperature of 10 °C). The greatest amount of denudation (5.0±1.6 km) occurred in the Sierra de Guadarrama. Accompanying rock uplift was 4.7±1.0 and 5.9±1.6 km in the eastern Gredos and Guadarrama, respectively. Most denudation in the Gredos occurred from the Middle Eocene to the Early Miocene and can be related to the N-S stress field, induced by the Pyrenean compression. In the Guadarrama, the greatest denudation was Pliocene to Recent of age and seems related to the ongoing NW-SE Betic compression. The fact that the formation of the E-W trending Gredos coincides with the N-S Pyrenean compression and the creation of the present day morphology of the NE-SW trending Guadarrama with the younger NW-SE Betic compression, indicates that they record the far field effects of Alpine plate tectonics on Iberia. The trend of pre-existing lineaments was of major importance in influencing the style and magnitude of these of far field effects.

  12. Unsteady two-layered fluid flow of conducting fluids in a channel between parallel porous plates under transverse magnetic field in a rotating system

    NASA Astrophysics Data System (ADS)

    Linga Raju, T.; Neela Rao, B.

    2016-05-01

    An unsteady MHD two-layered fluid flow of electrically conducting fluids in a horizontal channel bounded by two parallel porous plates under the influence of a transversely applied uniform strong magnetic field in a rotating system is analyzed. The flow is driven by a common constant pressure gradient in a channel bounded by two parallel porous plates, one being stationary and the other oscillatory. The two fluids are assumed to be incompressible, electrically conducting with different viscosities and electrical conductivities. The governing partial differential equations are reduced to the linear ordinary differential equations using two-term series. The resulting equations are solved analytically to obtain exact solutions for the velocity distributions (primary and secondary) in the two regions respectively, by assuming their solutions as a combination of both the steady state and time dependent components of the solutions. Numerical values of the velocity distributions are computed for different sets of values of the governing parameters involved in the study and their corresponding profiles are also plotted. The details of the flow characteristics and their dependence on the governing parameters involved, such as the Hartmann number, Taylor number, porous parameter, ratio of the viscosities, electrical conductivities and heights are discussed. Also an observation is made how the velocity distributions vary with the rotating hydromagnetic interaction in the case of steady and unsteady flow motions. The primary velocity distributions in the two regions are seen to decrease with an increase in the Taylor number, but an increase in the Taylor number causes a rise in secondary velocity distributions. It is found that an increase in the porous parameter decreases both the primary and secondary velocity distributions in the two regions.

  13. Blue Willow Story Plates

    ERIC Educational Resources Information Center

    Fontes, Kris

    2009-01-01

    In the December 1997 issue of "SchoolArts" is a lesson titled "Blue Willow Story Plates" by Susan Striker. In this article, the author shares how she used this lesson with her middle-school students many times over the years. Here, she describes a Blue Willow plate painting project that her students made.

  14. Earthquakes and plate tectonics

    USGS Publications Warehouse

    Spall, H.

    1977-01-01

    An explanation is to be found in plate tectonics, a concept which has revolutionized thinking in the Earth sciences in the last 10 years. The theory of plate tectonics combines many of the ideas about continental drift (originally proposed in 1912 by Alfred Wegener in Germany) and sea-floor spreading (suggested originally by Harry Hess of Princeton University). 

  15. Turbine vane plate assembly

    DOEpatents

    Schiavo Jr., Anthony L.

    2006-01-10

    A turbine vane assembly includes a turbine vane having first and second shrouds with an elongated airfoil extending between. Each end of the airfoil transitions into a shroud at a respective junction. Each of the shrouds has a plurality of cooling passages, and the airfoil has a plurality of cooling passages extending between the first and second shrouds. A substantially flat inner plate and an outer plate are coupled to each of the first and second shrouds so as to form inner and outer plenums. Each inner plenum is defined between at least the junction and the substantially flat inner plate; each outer plenum is defined between at least the substantially flat inner plate and the outer plate. Each inner plenum is in fluid communication with a respective outer plenum through at least one of the cooling passages in the respective shroud.

  16. A new control and data acquisition system for cosmic ray telescopes based on multigap resistive plate chambers

    NASA Astrophysics Data System (ADS)

    Avanzini, C.; Bossini, E.; Paoletti, R.; Partini, S.; Pilo, F.; Tazzioli, A.

    2013-08-01

    The upcoming phase of expansion of the telescope network of the EEE project has recently inspired a proposal of redesign of the detector control and monitoring, as well as data acquisition interfaces. The new design results in a very compact and versatile system with a significant cost reduction. The system architecture is described, with emphasis on the integration of data acquisition, trigger and GPS into a single unit. Performances of a fully functional prototype are also reported, as well as plans for future developments.

  17. Bending equation for a quasianisotropic plate

    NASA Astrophysics Data System (ADS)

    Shachnev, V. A.

    2010-10-01

    In the framework of the linear theory of elasticity, an exact bending equation is obtained for the median plane of a plate whose material is a monoclinic system with the axis of symmetry perpendicular to the plate plane. As an example, the equation of the median plane of an isotropic plate is considered; the operator of this equation coincides with the operator of Sophie Germain's approximate equation. As the plate thickness tends to zero, the right-hand side of the equation is asymptotically equivalent to the right-hand side of the approximate equation. In addition, equations relating the median plane transverse stresses and the total stresses in the plate boundary planes to the median plane deflexions are obtained.

  18. Thyroid hormone and the growth plate.

    PubMed

    Shao, Yvonne Y; Wang, Lai; Ballock, R Tracy

    2006-12-01

    Thyroid hormone was first identified as a potent regulator of skeletal maturation at the growth plate more than forty years ago. Since that time, many in vitro and in vivo studies have confirmed that thyroid hormone regulates the critical transition between cell proliferation and terminal differentiation in the growth plate, specifically the maturation of growth plate chondrocytes into hypertrophic cells. However these studies have neither identified the molecular mechanisms involved in the regulation of skeletal maturation by thyroid hormone, nor demonstrated how the systemic actions of thyroid hormone interface with the local regulatory milieu of the growth plate. This article will review our current understanding of the role of thyroid hormone in regulating the process of endochondral ossification at the growth plate, as well as what is currently known about the molecular mechanisms involved in this regulation.

  19. Importance of the Small-Scale Processes Melting, Plate Boundary Formation and Mineralogy on the Large-Scale, Long-Term Thermo-Chemical Evolution of Earth's Mantle-Plate System

    NASA Astrophysics Data System (ADS)

    Tackley, P.

    2015-12-01

    Seismic observations of the deep Earth reveal the presence of two large low shear velocity provinces (LLSVPs) that are typically inferred to be dense chemically-distinct material, as well as discontinuities that are typically linked to the post-perovskite (pPv) phase transition. Several possible origins of chemically-dense material have been proposed, including recycling of mid-ocean ridge basalt (MORB), primordial differentiation events, crystallisation of a basal magma ocean, or some combination of these creating a basal melange (BAM; Tackley 2012 Earth Sci. Rev.). Each of these possibilities would result in a different composition hence different mineralogy. In order to constrain this we have been running calculations of thermo-chemical mantle evolution over 4.5 billion years that include melting-induced differentiation, plate tectonics induced by strongly temperature-dependent viscosity and plastic yielding, core cooling and compressibility with reasonable assumptions about the pressure-dependence of other material properties. Some of our simulations start from a magma ocean state so initial layering is developed self-consistently. Already-published results (Nakagawa et al., 2009 GCubed, 2010 PEPI, 2012 GCubed) already indicate the importance of exact MORB composition on the amount of MORB segregating above the CMB, which in turn influences mantle thermal structure and the evolution of the core and geodynamo. In more recent results we have been additionally including primordial material. We find that melting-induced differentiation has several first-order effects on the dynamics, including (i) making plate tectonics easier (through stresses associated with lateral variations in crustal thickness) and (ii) reducing heat flux through the CMB (due to the build-up of dense material above the CMB); also (iii) tectonic mode (continuous plate tectonics, episodic lid or stagnant lid) also makes a first-order difference to mantle structure and dynamics. This emphasises

  20. Vacuum fusion bonding of glass plates

    DOEpatents

    Swierkowski, Steve P.; Davidson, James C.; Balch, Joseph W.

    2000-01-01

    An improved apparatus and method for vacuum fusion bonding of large, patterned glass plates. One or both glass plates are patterned with etched features such as microstructure capillaries and a vacuum pumpout moat, with one plate having at least one hole therethrough for communication with a vacuum pumpout fixture. High accuracy alignment of the plates is accomplished by a temporary clamping fixture until the start of the fusion bonding heat cycle. A complete, void-free fusion bond of seamless, full-strength quality is obtained through the plates; because the glass is heated well into its softening point and because of a large, distributed force that is developed that presses the two plates together from the difference in pressure between the furnace ambient (high pressure) and the channeling and microstructures in the plates (low pressure) due to the vacuum drawn. The apparatus and method may be used to fabricate microcapillary arrays for chemical electrophoresis; for example, any apparatus using a network of microfluidic channels embedded between plates of glass or similar moderate melting point substrates with a gradual softening point curve, or for assembly of glass-based substrates onto larger substrates, such as in flat panel display systems.

  1. Vacuum fusion bonding of glass plates

    DOEpatents

    Swierkowski, Steve P.; Davidson, James C.; Balch, Joseph W.

    2001-01-01

    An improved apparatus and method for vacuum fusion bonding of large, patterned glass plates. One or both glass plates are patterned with etched features such as microstructure capillaries and a vacuum pumpout moat, with one plate having at least one hole therethrough for communication with a vacuum pumpout fixture. High accuracy alignment of the plates is accomplished by a temporary clamping fixture until the start of the fusion bonding heat cycle. A complete, void-free fusion bond of seamless, full-strength quality is obtained through the plates; because the glass is heated well into its softening point and because of a large, distributed force that is developed that presses the two plates together from the difference in pressure between the furnace ambient (high pressure) and the channeling and microstructures in the plates (low pressure) due to the vacuum drawn. The apparatus and method may be used to fabricate microcapillary arrays for chemical electrophoresis; for example, any apparatus using a network of microfluidic channels embedded between plates of glass or similar moderate melting point substrates with a gradual softening point curve, or for assembly of glass-based substrates onto larger substrates, such as in flat panel display systems.

  2. A liquid phase based C. elegans behavioral analysis system identifies motor activity loss in a nematode Parkinson's disease model.

    PubMed

    Zheng, Maohua; Gorelenkova, Olga; Yang, Jiong; Feng, Zhaoyang

    2012-03-15

    Motor activity of Caenorhabditis elegans is widely used to study the mechanisms ranging from basic neuronal functions to human neurodegenerative diseases. It may also serve as a paradigm to screen for potential therapeutic reagents treating these diseases. Here, we developed an automated, 96-well plate and liquid phase based system that quantifies nematode motor activity in real time. Using this system, we identified an adult-onset, ageing-associated motor activity loss in a transgenic nematode line expressing human pathogenic G2019S mutant LRRK2 (leucine-rich repeat kinase 2), the leading genetic cause of Parkinson's disease characterized by dopaminergic neurodegeneration associated motor deficient mainly in elder citizens. Thus, our system may be used as a platform to screen for potential therapeutic drugs treating Parkinson's disease. It can also be used to monitor motor activity of nematodes in liquid phase at similar scenario.

  3. Characterisation of a PdCl 2/SnCl 2 electroless plating catalyst system adsorbed on barium titanate-based electroactive ceramics

    NASA Astrophysics Data System (ADS)

    Meenan, B. J.; Brown, N. M. D.; Wilson, J. W.

    1994-03-01

    A PdCl 2/SnCl 2 metallisation catalyst system, of the type used to activate non-conducting surfaces for electroless metal deposition, has been characterised by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). The substrate is a barium titanate (BaTiO 3)-based electroactive ceramic of the type used in the fabrication of multilayer ceramic capacitors (MLCC). The treatment of the substrate surface with the PdCl 2/SnCl 2 "sensitiser" solution leads to the adsorption of catalytically inactive compounds of palladium and tin. Subsequent treatment of this surface with an "accelerator" solution removes excess oxides, hydroxides and salts of tin thereby leaving the active catalyst species, Pd xSn y, on the surface. Such sites, on exposure to the appropriete electroless plating bath, are then responsible for the metal deposition. In this study, the chemical state and relative quantities of the various surface species present after each of the processing stages have been determined by XPS. The surface roughness of the substrate results in less of the tin compounds present thereon being removed on washing the catalysed surface in the accelerator solution than normally reported for such systems, thereby affecting the measured Pd: Sn ratio. SEM studies show that the accelerator solution treatment generates crystalline areas, which may be a result of coagulation of the Pd xSn y particles present, in the otherwise amorphous catalyst coating.

  4. Evaluation of three-dimensional cultured HepG2 cells in a nano culture plate system: an in vitro human model of acetaminophen hepatotoxicity.

    PubMed

    Aritomi, Kohei; Ishitsuka, Yoichi; Tomishima, Yoshiro; Shimizu, Daisuke; Abe, Nazuki; Shuto, Tsuyoshi; Irikura, Mitsuru; Kai, Hirofumi; Irie, Tetsumi

    2014-01-01

    Overdoses of acetaminophen (paracetamol, N-acetyl-p-aminophenol; APAP) cause severe liver injury, yet there is no common or high throughput in vitro human APAP model. This study examined the characteristics and usefulness of HepG2 cells grown in a nano culture plate (NCP) system, a three-dimensional culture method, as an in vitro human model for APAP-induced hepatotoxicity. The NCP-cultured HepG2 cells showed higher expression of mRNA and protein levels of cytochrome P450 2E1, which metabolizes APAP to a toxic metabolite, APAP-cysteine adduct formation, and higher sensitivity against APAP-induced cell injury compared with conventionally cultured cells. We demonstrated that treatment of APAP in NCP-cultured HepG2 cells shows key mechanistic features of APAP-induced hepatotoxicity, such as decreases in intracellular glutathione and mitochondrial membrane potential, activation of JNK, and cellular injury; and pharmacological agents, such as Cyclosporine A (a mitochondrial permeability transition inhibitor) and SP600125 (a JNK inhibitor), prevented cell injury induced by APAP exposure. In addition, the antidote of APAP-induced hepatotoxicity, N-acetylcysteine, could attenuate cellular injury induced by APAP in NCP-cultured HepG2 cells. We suggest that cellular injury induced by APAP treatment using an NCP-HepG2 system is a useful human model to study mechanisms and screen drug candidates of APAP-induced hepatotoxicity.

  5. Development of Magnetically Excited Flexural Plate Wave Devices for Implementation as Physical, Chemical, and Acoustic Sensors, and as Integrated Micro-Pumps for Sensored Systems

    NASA Astrophysics Data System (ADS)

    Schubert, W. K.; Mitchell, M. A.; Graf, D. C.; Shul, R. J.

    2002-05-01

    The magnetically excited flexural plate wave (mag-FPW) device has great promise as a versatile sensor platform. FPW's can have better sensitivity at lower operating frequencies than surface acoustic wave (SAW) devices. Lower operating frequency simplifies the control electronics and makes integration of sensor with electronics easier. Magnetic rather than piezoelectric excitation of the FPW greatly simplifies the device structure and processing by eliminating the need for piezoelectric thin films, also simplifying integration issues. The versatile mag-FPW resonator structure can potentially be configured to fulfill a number of critical functions in an autonomous sensored system. As a physical sensor, the device can be extremely sensitive to temperature, fluid flow, strain, acceleration and vibration. By coating the membrane with self-assembled monolayers (SAMs), or polymer films with selective absorption properties (originally developed for SAW sensors), the mass sensitivity of the FPW allows it to be used as biological or chemical sensors. Yet another critical need in autonomous sensor systems is the ability to pump fluid. FPW structures can be configured as micro-pumps. This report describes work done to develop mag-FPW devices as physical, chemical, and acoustic sensors, and as micro-pumps for both liquid and gas-phase analytes to enable new integrated sensing platform.

  6. Earthquakes and plate tectonics.

    USGS Publications Warehouse

    Spall, H.

    1982-01-01

    Earthquakes occur at the following three kinds of plate boundary: ocean ridges where the plates are pulled apart, margins where the plates scrape past one another, and margins where one plate is thrust under the other. Thus, we can predict the general regions on the earth's surface where we can expect large earthquakes in the future. We know that each year about 140 earthquakes of magnitude 6 or greater will occur within this area which is 10% of the earth's surface. But on a worldwide basis we cannot say with much accuracy when these events will occur. The reason is that the processes in plate tectonics have been going on for millions of years. Averaged over this interval, plate motions amount to several mm per year. But at any instant in geologic time, for example the year 1982, we do not know, exactly where we are in the worldwide cycle of strain build-up and strain release. Only by monitoring the stress and strain in small areas, for instance, the San Andreas fault, in great detail can we hope to predict when renewed activity in that part of the plate tectonics arena is likely to take place. -from Author

  7. Three-thrust fault system at the plate suture of arc-continent collision in the southernmost Longitudinal Valley, eastern Taiwan

    NASA Astrophysics Data System (ADS)

    Lee, J.; Chen, H.; Hsu, Y.; Yu, S.

    2013-12-01

    Active faults developed into a rather complex three-thrust fault system at the southern end of the narrow Longitudinal Valley in eastern Taiwan, a present-day on-land plate suture between the Philippine Sea plate and Eurasia. Based on more than ten years long geodetic data (including GPS and levelling), field geological investigation, seismological data, and regional tomography, this paper aims at elucidating the architecture of this three-thrust system and the associated surface deformation, as well as providing insights on fault kinematics, slip behaviors and implications of regional tectonics. Combining the results of interseismic (secular) horizontal and vertical velocities, we are able to map the surface traces of the three active faults in the Taitung area. The west-verging Longitudinal Valley Fault (LVF), along which the Coastal Range of the northern Luzon arc is thrusting over the Central Range of the Chinese continental margin, braches into two active strands bounding both sides of an uplifted, folded Quaternary fluvial deposits (Peinanshan massif) within the valley: the Lichi fault to the east and the Luyeh fault to the west. Both faults are creeping, to some extent, in the shallow surface level. However, while the Luyeh fault shows nearly pure thrust type, the Lichi fault reveals transpression regime in the north and transtension in the south end of the LVF in the Taitung plain. The results suggest that the deformation in the southern end of the Longitudinal Valley corresponds to a transition zone from present arc-collision to pre-collision zone in the offshore SE Taiwan. Concerning the Central Range, the third major fault in the area, the secular velocities indicate that the fault is mostly locked during the interseismic period and the accumulated strain would be able to produce a moderate earthquake, such as the example of the 2006 M6.1 Peinan earthquake, expressed by an oblique thrust (verging toward east) with significant left-lateral strike slip

  8. Pixelated neutron image plates

    NASA Astrophysics Data System (ADS)

    Schlapp, M.; Conrad, H.; von Seggern, H.

    2004-09-01

    Neutron image plates (NIPs) have found widespread application as neutron detectors for single-crystal and powder diffraction, small-angle scattering and tomography. After neutron exposure, the image plate can be read out by scanning with a laser. Commercially available NIPs consist of a powder mixture of BaFBr : Eu2+ and Gd2O3 dispersed in a polymer matrix and supported by a flexible polymer sheet. Since BaFBr : Eu2+ is an excellent x-ray storage phosphor, these NIPs are particularly sensitive to ggr-radiation, which is always present as a background radiation in neutron experiments. In this work we present results on NIPs consisting of KCl : Eu2+ and LiF that were fabricated into ceramic image plates in which the alkali halides act as a self-supporting matrix without the necessity for using a polymeric binder. An advantage of this type of NIP is the significantly reduced ggr-sensitivity. However, the much lower neutron absorption cross section of LiF compared with Gd2O3 demands a thicker image plate for obtaining comparable neutron absorption. The greater thickness of the NIP inevitably leads to a loss in spatial resolution of the image plate. However, this reduction in resolution can be restricted by a novel image plate concept in which a ceramic structure with square cells (referred to as a 'honeycomb') is embedded in the NIP, resulting in a pixelated image plate. In such a NIP the read-out light is confined to the particular illuminated pixel, decoupling the spatial resolution from the optical properties of the image plate material and morphology. In this work, a comparison of experimentally determined and simulated spatial resolutions of pixelated and unstructured image plates for a fixed read-out laser intensity is presented, as well as simulations of the properties of these NIPs at higher laser powers.

  9. New Method for Accurate Calibration of Micro-Channel Plate based Detection Systems and its use in the Fast Plasma Investigation of NASA's Magnetospheric MultiScale Mission

    NASA Astrophysics Data System (ADS)

    Gliese, U.; Avanov, L. A.; Barrie, A.; Kujawski, J. T.; Mariano, A. J.; Tucker, C. J.; Chornay, D. J.; Cao, N. T.; Zeuch, M.; Pollock, C. J.; Jacques, A. D.

    2013-12-01

    The Fast Plasma Investigation (FPI) of the NASA Magnetospheric MultiScale (MMS) mission employs 16 Dual Electron Spectrometers (DESs) and 16 Dual Ion Spectrometers (DISs) with 4 of each type on each of 4 spacecraft to enable fast (30ms for electrons; 150ms for ions) and spatially differentiated measurements of full the 3D particle velocity distributions. This approach presents a new and challenging aspect to the calibration and operation of these instruments on ground and in flight. The response uniformity and reliability of their calibration and the approach to handling any temporal evolution of these calibrated characteristics all assume enhanced importance in this application, where we attempt to understand the meaning of particle distributions within the ion and electron diffusion regions. Traditionally, the micro-channel plate (MCP) based detection systems for electrostatic particle spectrometers have been calibrated by setting a fixed detection threshold and, subsequently, measuring a detection system count rate plateau curve to determine the MCP voltage that ensures the count rate has reached a constant value independent of further variation in the MCP voltage. This is achieved when most of the MCP pulse height distribution (PHD) is located at higher values (larger pulses) than the detection amplifier threshold. This method is adequate in single-channel detection systems and in multi-channel detection systems with very low crosstalk between channels. However, in dense multi-channel systems, it can be inadequate. Furthermore, it fails to fully and individually characterize each of the fundamental parameters of the detection system. We present a new detection system calibration method that enables accurate and repeatable measurement and calibration of MCP gain, MCP efficiency, signal loss due to variation in gain and efficiency, crosstalk from effects both above and below the MCP, noise margin, and stability margin in one single measurement. The fundamental

  10. The Clinical Usefulness of Ultrasound-Aided Fixation Using an Absorbable Plate System in Patients with Zygomatico-Maxillary Fracture

    PubMed Central

    Park, Jun Hyung

    2013-01-01

    Background Ultrasound-aided fixation is a recently developed alternative method of treatment of zygomatico-maxillary (ZM) fracture, and it can resolve the problems of excessive torsion force and subsequent fractures of screws. We conducted this study to evaluate the clinical usefulness of ultrasound-aided fixation as compared with the conventional fixation method using a drill and an expander in patients with ZM fracture. Methods We conducted a retrospective study in 35 patients with ZM fracture who had been treated at our hospital during a period ranging from March of 2008 to December of 2010. We divided them into two groups: an ultrasound-aided fixation group, comprising 13 patients who underwent ultrasound-aided fixation (SonicWeld Rx, KLS Martin), and a conventional group, comprising 22 patients who underwent conventional fixation (Biosorb FX, Linvatec Biomaterials Ltd.). We compared such variables as sex, direction, age at operation, follow-up period, operation duration, number of fixed holes, and time to discharge between the two groups. Results The ultrasound-aided fixation reduced the operation duration by about 30 minutes as compared with that of conventional fixation. There was no significant difference in follow-up period, number of fixed holes, or time to discharge between the two groups. Furthermore, there were no complications in either group. Conclusions The ultrasound-aided fixation of fractured ZM bone using an absorbable implant system is safe and effective in promptly reducing the bone fracture and providing satisfactory cosmetic outcomes over time. PMID:23898427

  11. Designing Assemblies Of Plates

    NASA Technical Reports Server (NTRS)

    Williams, F. W.; Kennedy, D.; Butler, R.; Aston, G.; Anderson, M. S.

    1992-01-01

    VICONOPT calculates vibrations and instabilities of assemblies of prismatic plates. Designed for efficient, accurate analysis of buckling and vibration, and for optimum design of panels of composite materials. Written in FORTRAN 77.

  12. Plate tectonics: Metamorphic myth

    NASA Astrophysics Data System (ADS)

    Korenaga, Jun

    2016-01-01

    Clear evidence for subduction-induced metamorphism, and thus the operation of plate tectonics on the ancient Earth has been lacking. Theoretical calculations indicate that we may have been looking for something that cannot exist.

  13. Violin plate modes.

    PubMed

    Gough, Colin

    2015-01-01

    As the first step toward developing a generic model for the acoustically radiating vibrational modes of the violin and related instruments, the modes of both freely supported and edge-constrained top and back plates have been investigated as functions of shape, arching height, elastic anisotropy, the f-holes and associated island area, thickness graduations, and the additional boundary constraints of the ribs, soundpost, and bass-bar present in the assembled instrument. Comsol shell structure finite element software has been used as a quasi-experimental tool, with physical and geometric properties varied smoothly, often over several orders of magnitude, allowing the development of the plate modes to be followed continuously from those of an initially square plate to those of doubly-arched, guitar-shaped, orthotropic plates and their dependence on all the above factors.

  14. Tectonic Plate Movement.

    ERIC Educational Resources Information Center

    Landalf, Helen

    1998-01-01

    Presents an activity that employs movement to enable students to understand concepts related to plate tectonics. Argues that movement brings topics to life in a concrete way and helps children retain knowledge. (DDR)

  15. Reduction of astrometric plates

    NASA Technical Reports Server (NTRS)

    Stock, J.

    1984-01-01

    A rapid and accurate method for the reduction of comet or asteroid plates is described. Projection equations, scale length correction, rotation of coordinates, linearization, the search for additional reference stars, and the final solution are examined.

  16. What's On Your Plate?

    MedlinePlus

    ... Table of Contents What's On Your Plate? Smart Food Choices for Healthy Aging www.nia.nih.gov/health/ ... calories in" and "calories out," and making good food choices as you age. Shopping Tips See how planning ...

  17. Feynman's wobbling plate

    NASA Astrophysics Data System (ADS)

    Tuleja, Slavomir; Gazovic, Boris; Tomori, Alexander; Hanc, Jozef

    2007-03-01

    In the book Surely You Are Joking, Mr. Feynman! Richard Feynman tells a story of a Cornell cafeteria plate being tossed into the air. As the plate spun, it wobbled. Feynman noticed a relation between the two motions. He solved the motion of the plate by using the Lagrangian approach. This solution didn't satisfy him. He wanted to understand the motion of the plate by analyzing the motion of its individual particles and the forces acting on them. He was successful, but he didn't tell us how he did it. We provide an elementary explanation for the two-to-one ratio of wobble to spin frequencies, based on an analysis of the motion of the particles and the forces acting on them. We also demonstrate the power of numerical simulation and computer animation to provide insight into a physical phenomenon and guidance on how to do the analysis.

  18. Flat plate solar oven

    SciTech Connect

    Parikh, M.

    1981-01-01

    The construction of an Indian Rs. 186 (US $20.33) flat-plate solar oven is described. Detailed drawings are provided and relevant information on cooking times and temperature for different foods is given.

  19. Involvement of the German Research Center for GeoSciences (GFZ) in the EPOS Implementation Phase 2015-18 (European Plate Observing System)

    NASA Astrophysics Data System (ADS)

    Hoffmann, T. L.; Lauterjung, J.

    2015-12-01

    Under the Horizon 2020 Programme INFRADEV-3, the European Commission (EC) has awarded a prioritized grant for the establishment of the European Plate Observing System (EPOS) during a four-year Implementation Phase 2015-18. As laid in detail during the EPOS Preparatory Phase 2010-14, the EPOS cyberinfrastructure will be established as an ERIC (European Research Infrastructure Consortium) and it will encompass the implementation of both the EPOS Integrated Core Services (ICS) for solid Earth Science and a multitude of EPOS Thematic Core Services (TCS). As one of the 29 awardees of the EC grant, the German Research Center for Geosciences (GFZ) will play an important role in the implementation of EPOS and its Thematic and Integrated Core Services. The presented poster will give an overview of GFZ's involvement in the different Work Packages, including administrative tasks (WP3 Harmonization) as well as the technical implementation efforts (WP7 ICS Development, WP8 Seismology, WP11 Volcano Observations, WP12 Satellite Data, WP13 Geomagnetic Observations, WP14 Anthropogenic Hazards, WP15 Geological Information and Modelling, WP16 Multi-Scale Laboratories and WP17 Geo Energy Test Beds).

  20. High-throughput system for screening of high L-lactic acid-productivity strains in deep-well microtiter plates.

    PubMed

    Lv, Xiangyun; Song, Jiali; Yu, Bo; Liu, Huilan; Li, Chao; Zhuang, Yingping; Wang, Yonghong

    2016-11-01

    For strain improvement, robust and scalable high-throughput cultivation systems as well as simple and rapid high-throughput detection methods are crucial. However, most of the screening methods for lactic acid bacteria (LAB) strains were conducted in shake flasks and detected by high-performance liquid chromatography (HPLC), making the screening program laborious, time-consuming and costly. In this study, an integrated strategy for high-throughput screening of high L-lactic acid-productivity strains by Bacillus coagulans in deep-well microtiter plates (MTPs) was developed. The good agreement of fermentation results obtained in the MTPs platform with shake flasks confirmed that 24-well U-bottom MTPs could well alternate shake flasks for cell cultivation as a scale-down tool. The high-throughput pH indicator (bromocresol green) and L-lactate oxidase (LOD) assays were subsequently developed to qualitatively and quantitatively analyze L-lactic acid concentration. Together with the color halos method, the pH indicator assay and LOD assay, the newly developed three-step screening strategy has greatly accelerated the screening process for LAB strains with low cost. As a result, two high L-lactic acid-productivity mutants, IH6 and IIIB5, were successfully screened out, which presented, respectively, 42.75 and 46.10 % higher productivities than that of the parent strain in a 5-L bioreactor.

  1. Dynamics of an inverted flexible plate in a uniform flow

    NASA Astrophysics Data System (ADS)

    Tang, Chao; Liu, Nan-Sheng; Lu, Xi-Yun

    2015-07-01

    The dynamics of an inverted flexible plate with a free leading-edge and a fixed trailing-edge in a uniform flow has been studied numerically by an immersed boundary-lattice Boltzmann method for the fluid flow and a finite element method for the plate deformation. Mechanisms underlying the dynamics of the fluid-plate system are elucidated systematically. A series of distinct states of the plate deformation and motion are identified and can be described as straight, flapping, deflected, deflected-flapping, and asymmetric-flapping states. Which state to occur depends mainly on the bending stiffness and aspect ratio of the plate. The forces exerted on the plate and the elastic strain energy of the plate are analyzed. It is found that the flapping state can improve the conversion of fluid kinetic energy to elastic strain energy. In addition, the effects of the mass ratio of the plate and the fluid, the Reynolds number, and the angle of attack of the uniform flow on the dynamics and the elastic strain energy of flexible plate are also investigated in detail. The vortical structures around the plate are given to discuss the connection of the evolution of vortices with the plate deformation and motion. The results obtained in this study provide physical insight into the understanding of the mechanisms on the dynamics of the fluid-plate system.

  2. Fractal multifiber microchannel plates

    NASA Technical Reports Server (NTRS)

    Cook, Lee M.; Feller, W. B.; Kenter, Almus T.; Chappell, Jon H.

    1992-01-01

    The construction and performance of microchannel plates (MCPs) made using fractal tiling mehtods are reviewed. MCPs with 40 mm active areas having near-perfect channel ordering were produced. These plates demonstrated electrical performance characteristics equivalent to conventionally constructed MCPs. These apparently are the first MCPs which have a sufficiently high degree of order to permit single channel addressability. Potential applications for these devices and the prospects for further development are discussed.

  3. Positive battery plate

    NASA Technical Reports Server (NTRS)

    Rowlette, John R. (Inventor)

    1985-01-01

    The power characteristics of a lead acid battery are improved by incorporating a dispersion of 1 to 10% by weight of a thermodynamically stable conductivity additive, such as conductive tin oxide coated glass fibers (34) of filamentary glass wool (42) in the positive active layer (32) carried on the grid (30) of the positive plate (16). Positive plate potential must be kept high enough to prevent reduction of the tin oxide to tin by utilizing an oversized, precharged positive paste.

  4. Subduction Drive of Plate Tectonics

    NASA Astrophysics Data System (ADS)

    Hamilton, W. B.

    2003-12-01

    Don Anderson emphasizes that plate tectonics is self-organizing and is driven by subduction, which rights the density inversion generated as oceanic lithosphere forms by cooling of asthenosphere from the top. The following synthesis owes much to many discussions with him. Hinge rollback is the key to kinematics, and, like the rest of actual plate behavior, is incompatible with bottom-up convection drive. Subduction hinges (which are under, not in front of, thin leading parts of arcs and overriding plates) roll back into subducting plates. The Pacific shrinks because bounding hinges roll back into it. Colliding arcs, increasing arc curvatures, back-arc spreading, and advance of small arcs into large plates also require rollback. Forearcs of overriding plates commonly bear basins which preclude shortening of thin plate fronts throughout periods recorded by basin strata (100 Ma for Cretaceous and Paleogene California). This requires subequal rates of advance and rollback, and control of both by subduction. Convergence rate is equal to rates of rollback and advance in many systems but is greater in others. Plate-related circulation probably is closed above 650 km. Despite the popularity of concepts of plumes from, and subduction into, lower mantle, there is no convincing evidence for, and much evidence against, penetration of the 650 in either direction. That barrier not only has a crossing-inhibiting negative Clapeyron slope but also is a compositional boundary between fractionated (not "primitive"), sluggish lower mantle and fertile, mobile upper mantle. Slabs sink more steeply than they dip. Slabs older than about 60 Ma when their subduction began sink to, and lie down on and depress, the 650-km discontinuity, and are overpassed, whereas younger slabs become neutrally buoyant in mid-upper mantle, into which they are mixed as they too are overpassed. Broadside-sinking old slabs push all upper mantle, from base of oceanic lithosphere down to the 650, back under

  5. High MS-compatibility of silver nitrate-stained protein spots from 2-DE gels using ZipPlates and AnchorChips for successful protein identification.

    PubMed

    Nebrich, Grit; Herrmann, Marion; Sagi, Dijana; Klose, Joachim; Giavalisco, Patrick

    2007-05-01

    The availability of easy-to-handle, sensitive, and cost-effective protein staining protocols for 2-DE, in conjunction with a high compatibility for subsequent MS analysis, is still a prerequisite for successful proteome research. In this article we describe a quick and easy-to-use methodological protocol based on sensitive, homogeneous, and MS-compatible silver nitrate protein staining, in combination with an in-gel digestion, employing the Millipore 96-well ZipPlate system for peptide preparation. The improved quality and MS compatibility of the generated protein digests, as compared to the otherwise weakly MS-compatible silver nitrate staining, were evaluated on real tissue samples by analyzing 192 Coomassie-stained protein spots against their counterparts from a silver-stained 2-DE gel. Furthermore, the applicability of the experimental setup was evaluated and demonstrated by the analysis of a large-scale MALDI-TOF MS experiment, in which we analyzed an additional ~1000 protein spots from 2-DE gels from mouse liver and mouse brain tissue.

  6. Ethics issues in scientific data and service provision: evidence and challenges for the European Plate Observing System (EPOS)

    NASA Astrophysics Data System (ADS)

    Cocco, Massimo; Freda, Carmela; Haslinger, Florian; Consortium, Epos

    2016-04-01

    Addressing Ethics issues is nowadays a relevant challenge for any initiative, program or project dealing with scientific data and products provision, access to services for scientific purposes and communication with different stakeholders, including society. This is corroborated by the evidence that Ethics has very high priority in EU funded research. Indeed, all the activities carried out under Horizon 2020 must comply with ethical principles and national, Union and international legislation. This implies that "For all activities funded by the European Union, Ethics is an integral part of research from beginning to end, and ethical compliance is seen as pivotal to achieve real research excellence." Here, we present the experience of EPOS, a public pan-European research infrastructure. EPOS aims at integrating data, data products, services and software (DDSS) for solid Earth science generated and provided by monitoring networks, observing systems and facilities belonging to European countries. EPOS fosters the integrated use of multidisciplinary solid Earth data to improve the understanding of physical and chemical processes controlling earthquakes, volcanic eruptions, tsunamis as well as those driving tectonics and surface dynamics. The EPOS integration plan will make significant contributions to understanding and mitigating geo-hazards, yielding data for hazard assessment, data products for engaging different stakeholders, and services for training, education and communication to society. Numerous national research infrastructures engaged in EPOS are deployed for the monitoring of areas prone to geo-hazards and for the surveillance of the national territory including areas used for exploiting geo-resources. The EPOS community is therefore already trained to provide services to public (civil defence agencies, local and national authorities) and private (petroleum industry, mining industry, geothermal companies, aviation security) stakeholders. Our ability to

  7. Evaluation of cytotoxicity of oils used in coenzyme Q10 Self-emulsifying Drug Delivery Systems (SEDDS).

    PubMed

    Palamakula, Anitha; Khan, Mansoor A

    2004-04-01

    The objective of the present study was to develop a suitable method for evaluation of cytotoxicity of the oils used in Self-Emulsified Drug Delivery Systems (SEDDS) using Coenzyme Q10 (Co Q10) as a model compound. For this purpose, three methods of sample preparation were tested, namely (i) suspensions, (ii) homogenization, and (iii) nanoemulsions of oils in Dulbecco's Modified Eagle's Media (DMEM). Studies were carried out by incubating the sample or control with Caco-2 cells grown on transwell insert systems as well as in flat bottom 96-well plates. The cell viability was assessed by using WST-1 and propidium iodide reagents while the monolayer integrity was assessed by mannitol permeability and Trans Epithelial Electrical Resistance (TEER). The cytotoxicity of oils was found to be dependent on the method of sample preparation; nanoemulsions being the least cytotoxic. In conclusion, nanoemulsification is a useful tool for cytotoxicity evaluation of substances, which exhibit poor aqueous/dimethyl sulfoxide (DMSO) solubility.

  8. Plated-through hole testing. Final repot

    SciTech Connect

    Abel, W.B.

    1980-05-01

    An improved microresistance testing method to measure the thickness of copper plating in plated-through holes of double-sided printed wiring boards is presented. A new probe design, combined with a microprocessor controller, makes it possible for a direct reading tester to provide variables data. Tester design and software programs for the system are included. Results of correlation studies, comparing the improved resistance method to cross-sectional measurements are presented. Limitations on the microresistance test method are discussed.

  9. Electrochemical cell and separator plate thereof

    DOEpatents

    Baker, Bernard S.; Dharia, Dilip J.

    1979-01-01

    A fuel cell includes a separator plate having first and second flow channels extending therethrough contiguously with an electrode and respectively in flow communication with the cell electrolyte and in flow isolation with respect to such electrolyte. In fuel cell system arrangement, the diverse type channels are suplied in common with process gas for thermal control purposes. The separator plate is readily formed by corrugation of integral sheet material.

  10. Electrochemical cell and separator plate thereof

    DOEpatents

    Baker, Bernard S.; Dharia, Dilip J.

    1979-10-02

    A fuel cell includes a separator plate having first and second flow channels extending there through contiguously with an electrode and respectively in flow communication with the cell electrolyte and in flow isolation with respect to such electrolyte. In fuel cell system arrangement, the diverse type channels are supplied in common with process gas for thermal control purposes. The separator plate is readily formed by corrugation of integral sheet material. 10 figs.

  11. Plate Boundary Observatory in Taiwan

    NASA Astrophysics Data System (ADS)

    Yu, S.; Tsai, C.

    2003-12-01

    The island of Taiwan is situated in the plate boundary zone between the Eurasian and the Philippine Sea plates. The Philippine Sea plate is subducting northwestward underneath the Eurasian plate along the Ryukyu Trench in the north, while the Eurasian plate underthrusts the Philippine Sea plate along the Manila Trench in the south. Taking advantage of the extremely high strain rate in the Taiwan area, an integrated National Science Council project, Plate Boundary Observatory in Taiwan (PBOT), was initiated following the idea of US PBO. The scientific goal of PBOT is to observe the crustal deformation on various temporal and spatial scales in the Taiwan plate boundary zone employing available state of the art techniques for measuring crustal strain. The techniques include seismology, Global Positioning System (GPS), Interferometric Synthetic Aperture Radar (InSAR), borehole strainmeter, and earthquake geology. They are complementary to each other and form a complete spectrum of measuring various periods of crustal strain. The process of crustal deformation is generally quite slow. To obtain a reliable result, we usually need to persist in the observations for several years or even decades. Thus the PBOT should be a long-term project. In the first phase of 3 years period from 2003 to 2006, we will focus on the two areas, i.e. the plate suture zone in the Longitudinal Valley area and the western Taiwan where the higher seismic hazard is expected. A five-year national program, entitled ­Program for Earthquake and Active-fault Research (PEAR)­" was initiated after the disastrous 1999 Chi-Chi earthquake (Mw 7.6). As part of the PEAR, a dense continuous GPS array consisting of 150 new and about 50 pre-existing stations will be completed in the Taiwan area by the end of 2005 through a joint effort by the Central Weather Bureau and the Institute of Earth Sciences, Academia Sinica. The 50 new stations are going to be evenly distributed around the Taiwan Island. The other

  12. Caribbean plate interactions

    SciTech Connect

    Ball, M. )

    1993-02-01

    Vector analysis of plate motions, derived from studies of Atlantic magnetic lineations and fracture zone trends, indicates the following relative movements between the Caribbean, North American, and South American Plates. (1) During Early Jurassic to Early Cretaceous, the North American Plate moved 1900 km westward and 900 km northward relative to the South American Plate. A broad zone including the Caribbean region, i.e., the zone between the North and South America Plates, was a site of left-lateral shear and north-south extension. (2) During Early Cretaceous to Late Cretaceous, the North American Mate moved an additional 1200 km westward relative to South America across this zone. (3) During Late Cretaceous to the end of the Eocene, the North American Plate moved 200 km westward and 400 km northward relative to the South American Plate. (4) From the end of the Eocene to near the end of the Miocene, North America converged on South America some 200 km and moved 100 km eastward relative to it. Through the Mesozoic and earliest Tertiary history of the Caribbean, the region was a shear zone within which left-lateral displacement exceeded 3000 km and north-south extension exceeded 1300 km. In regard to time, 80% of the history of the Caribbean region is one of north-south extension and left-lateral shear. In terms of space, 97% of the shear is left-lateral and the ratio of divergence versus convergence is 7 to 1. Thus, characterizing the Caribbean region, and the Atlantic to its east, as a zone of north-south extension and left-lateral shear, is a fair generalization.

  13. A cell-based screening system for detection of inhibitors toward mycobacterial cell wall core.

    PubMed

    Gao, Peng; Guan, Yan; Song, Danqing; Xiao, Chunling

    2009-06-01

    Mycobacterium tuberculosis and nonpathogenic bacteria, Corynebacterium glutamicum, possess a common and unusual cell wall architecture. A cell-based screening system was designed to identify novel compounds interacting with the synthesis, assembly or regulation of the M. tuberculosis cell wall. C. glutamicum was tested in a paired medium assay in 96-well plates with natural product extracts and pure chemical compounds in the presence and absence of the osmotic stabilizer, sorbitol and some ions. Growth was visually examined over a 12-h period and detected with a microplate reader for absorbance at 544 nm. Screening hits from the osmotic stabilizer rescue were then examined by mycolic acid analysis to confirm the effect on cell wall integrity.

  14. Improvements in Cold-Plate Fabrication

    NASA Technical Reports Server (NTRS)

    Zaffetti, Mark A.; Taddey, Edmund P.; Laurin, Michael B.; Chabebe, Natalia

    2012-01-01

    Five improvements are reported in cold-plate fabrication. This cold plate is part of a thermal control system designed to serve on space missions. The first improvement is the merging of the end sheets of the cold plate with the face sheets of the structural honeycomb panel. The cold plate, which can be a brazed assembly, uses the honeycomb face sheet as its end sheet. Thus, when the honeycomb panel is fabricated, the face sheet that is used is already part of the cold plate. In addition to reducing weight, costs, and steps, the main benefit of this invention is that it creates a more structurally sound assembly. The second improvement involves incorporation of the header into the closure bar to pass the fluid to a lower layer. Conventional designs have used a separate header, which increases the geometry of the system. The improvement reduces the geometry, thus allowing the cold plate to fit into smaller area. The third improvement eliminates the need of hose, tube, or manifold to supply the cooling fluid externally. The external arrangement can be easily damaged and is vulnerable to leakage. The new arrangement incorporates an internal fluid transfer tube. This allows the fluid to pass from one cold plate to the other without any exposed external features. The fourth improvement eliminates separate fabrication of cold plate(s) and structural members followed by a process of attaching them to each other. Here, the structural member is made of material that can be brazed just as that of the cold plate. Now the structural member and the cold plate can be brazed at the same time, creating a monolithic unit, and thus a more structurally sound assembly. Finally, the fifth improvement is the elimination of an additional welding step that can damage the braze joints. A tube section, which is usually welded on after the braze process, is replaced with a more structurally sound configuration that can be brazed at the same time as the rest of the cold plate.

  15. Effective characteristics of corrugated plates

    NASA Astrophysics Data System (ADS)

    Arkhangel'skii, A. F.; Gorbachev, V. I.

    2007-06-01

    Corrugated plates are widely used in modern constructions and structures, because they, in contrast to plane plates, possess greater rigidity. In many cases, such a plate can be modeled by a homogeneous anisotropic plate with certain effective flexural and tensional rigidities. Depending on the geometry of corrugations and their location, the equivalent homogeneous plate can also have rigidities of mutual influence. These rigidities allow one to take into account the influence of bending moments on the strain in the midplane and, conversely, the influence of longitudinal strains on the plate bending [1]. The behavior of the corrugated plate under the action of a load normal to the midsurface is described by equations of the theory of flexible plates with initial deflection. These equations form a coupled system of nonlinear partial differential equations with variable coefficients [2]. The dependence of the coefficients on the coordinates is determined by the corrugation geometry. In the case of a plate with periodic corrugation, the coefficients significantly vary within one typical element and depend on the values of local variables determined in each of the typical elements. There is a connection between the local and global variables, and therefore, the functions of local coordinates are simultaneously functions of global coordinates, which are sometimes called rapidly oscillating functions [3]. One of the methods for solving the equations with rapidly oscillating coefficients is the asymptotic method of small geometric parameter. The standard procedure of this method usually includes preparatory stages. At the first stage, as a rule, a rectangular periodicity cell is distinguished to be a typical element. At the second stage, the scale of global coordinates is changed so that the rectangular structure periodicity cells became square cells of size l × l. The third stage consists in passing to the dimensionless global coordinates relative to the plate

  16. Study on plate silencer with general boundary conditions

    NASA Astrophysics Data System (ADS)

    Liu, Gongmin; Zhao, Xiaochen; Zhang, Wenping; Li, Shuaijun

    2014-09-01

    A plate silencer consists of an expansion chamber with two side-branch rigid cavities covered by plates. Previous studies showed that, in a duct, the introduction of simply supported or clamped plates into an air conveying system could achieve broadband quieting from low to medium frequencies. In this study, analytical formulation is extended to the plate silencer with general boundary conditions. A set of static beam functions, which are a combination of sine series and third-order polynomial, is employed as the trial functions of the plate vibration velocity. Greens function and Kirchhoff-Helmholtz integral are used to solve the sound radiation in the duct and the cavity, and then the vibration velocity of the plate is obtained. Having obtained the vibration velocity, the pressure perturbations induced by the plate oscillation and the transmission loss are found. Optimization is carried out in order to obtain the widest stopband. The transmission loss calculated by the analytical method agrees closely with the result of the finite element method simulation. Further studies with regard to the plate under several different classical boundary conditions based on the validated model show that a clamped-free plate silencer has the worst stopband. Attempts to release the boundary restriction of the plate are also made to study its effect on sound reflection. Results show that a softer end for a clamped-clamped plate silencer helps increase the optimal bandwidth, while the same treatment for simply supported plate silencer will result in performance degradation.

  17. Biomechanical study of different plate configurations for distal humerus osteosynthesis.

    PubMed

    Bogataj, M; Kosel, F; Norris, R; Krkovic, M; Brojan, M

    2015-05-01

    Fractures of the distal humerus are most commonly fixed by open reduction and internal fixation, using plates and screws, either in a locking or in a non-locking construct. Three different plating systems are commonly used in practice. The most important differences between them are in plate orientation, which affects both the rigidity of the osteosynthesis and invasiveness of the surgical procedure. Unfortunately, there is no common agreement between surgeons about which plate configuration brings the best clinical outcome. In this study, we investigate the theoretical rigidity of plate osteosyntheses considering two types of AO/ASIF configurations (90° angle between plates), Mayo clinic (Acumed) configuration (180° between plates) and dorsal fixation of both plates. We also compared the results for cases with and without contact between the bone fragments. In the case of no bone contact, the Mayo clinic plate configuration is found to be the most rigid, followed by both AO/ASIF plate configurations, and the least rigid system is the Korosec plate configuration. On the other hand, no significant differences between all types of fixation configurations are found in cases with contact in-between the bone fragments. Our findings show that this contact is very important and can compensate for the lack of load carrying capacity of the implants. This could therefore incite other implant fixation solutions, leading to less invasive surgical procedures and consequently improved clinical outcome.

  18. Bipolar battery plate

    NASA Technical Reports Server (NTRS)

    Rowlette, John J. (Inventor)

    1987-01-01

    A liquid-impermeable plate (10) having through-plate conductivity with essentially zero resistance comprises an insulator sheet (12) having a series of spaced perforations (14) each of which contains a metal element (16) sealingly received into the perforation (14). A low-cost plate can readily be manufactured by punching a thermoplastic sheet (40) such as polypropylene with a punching tool (52), filling the apertures with led spheres (63) having a diameter smaller than the holes (50) but larger than the thickness of the sheet, sweeping excess spheres (62) off the sheet with a doctor blade (60) and then pressing a heated platen (74) onto the sheet to swage the spheres into a cylindrical shape and melt the surrounding resin to form a liquid-impermeable collar (4) sealing the metal into the sheet.

  19. A Comparative Outcomes Study Using the Volar Locking Plating System for Distal Radius Fractures in both Young Adults and Adults Older than 60 Years

    PubMed Central

    Chung, Kevin C.; Squitieri, Lee; Kim, H. Myra

    2015-01-01

    Purpose Despite the high prevalence and impact of distal radius fractures (DRFs) on older patients, the current available literature regarding DRFs in older adults lacks adequate comparative treatment data. The purpose of this prospective, controlled outcomes study is to compare outcomes using the volar locking plating system (VLPS) for DRFs in both older and younger adults. Methods Consecutive, eligible patients were enrolled into our prospective study over a two-year period on the basis of strict inclusion/exclusion criteria. Subjects were entered into two cohorts based on age: 20–40 years and ≥ 60 years. Patient outcomes and complication rates were evaluated at three, six and twelve months after surgery. Outcome measures included the Michigan Hand Outcomes Questionnaire (MHQ), grip strength, active wrist and forearm range of motion, the Jebsen-Taylor test, and radiographic parameters. Results 55 patients (30 young and 25 older adults) with unilateral, inadequately reduced DRFs were enrolled and received surgical treatment with the VLPS. We observed no statistically significant difference in any of the outcomes for all three follow-up periods. While older age patients continued to improve throughout their twelve month postoperative visits, younger patients achieved their maximum recovery during the six month follow-up period, suggesting different recovery patterns. At the twelve-month assessment, older patients were able to achieve a higher mean MHQ score than their younger counterparts (normalized mean: 85% and 82%, respectively). Complication rates were similar between the two groups for all three time periods, with most occurring on or before the three month postoperative visit. Conclusions This study indicates that the VLPS is successful in managing DRFs in older patients and without increased complications compared to younger patients. For the older patients without prohibitive surgical risks, internal fixation using the VLPS yields comparable outcomes

  20. Image simulation for automatic license plate recognition

    NASA Astrophysics Data System (ADS)

    Bala, Raja; Zhao, Yonghui; Burry, Aaron; Kozitsky, Vladimir; Fillion, Claude; Saunders, Craig; Rodríguez-Serrano, José

    2012-01-01

    Automatic license plate recognition (ALPR) is an important capability for traffic surveillance applications, including toll monitoring and detection of different types of traffic violations. ALPR is a multi-stage process comprising plate localization, character segmentation, optical character recognition (OCR), and identification of originating jurisdiction (i.e. state or province). Training of an ALPR system for a new jurisdiction typically involves gathering vast amounts of license plate images and associated ground truth data, followed by iterative tuning and optimization of the ALPR algorithms. The substantial time and effort required to train and optimize the ALPR system can result in excessive operational cost and overhead. In this paper we propose a framework to create an artificial set of license plate images for accelerated training and optimization of ALPR algorithms. The framework comprises two steps: the synthesis of license plate images according to the design and layout for a jurisdiction of interest; and the modeling of imaging transformations and distortions typically encountered in the image capture process. Distortion parameters are estimated by measurements of real plate images. The simulation methodology is successfully demonstrated for training of OCR.

  1. Modeling the hydrodynamics of Phloem sieve plates.

    PubMed

    Jensen, Kaare Hartvig; Mullendore, Daniel Leroy; Holbrook, Noel Michele; Bohr, Tomas; Knoblauch, Michael; Bruus, Henrik

    2012-01-01

    Sieve plates have an enormous impact on the efficiency of the phloem vascular system of plants, responsible for the distribution of photosynthetic products. These thin plates, which separate neighboring phloem cells, are perforated by a large number of tiny sieve pores and are believed to play a crucial role in protecting the phloem sap from intruding animals by blocking flow when the phloem cell is damaged. The resistance to the flow of viscous sap in the phloem vascular system is strongly affected by the presence of the sieve plates, but the hydrodynamics of the flow through them remains poorly understood. We propose a theoretical model for quantifying the effect of sieve plates on the phloem in the plant, thus unifying and improving previous work in the field. Numerical simulations of the flow in real and idealized phloem channels verify our model, and anatomical data from 19 plant species are investigated. We find that the sieve plate resistance is correlated to the cell lumen resistance, and that the sieve plate and the lumen contribute almost equally to the total hydraulic resistance of the phloem translocation pathway.

  2. Plate-mantle coupling from post-Pangea plate kinematics

    NASA Astrophysics Data System (ADS)

    Zahirovic, Sabin; Dietmar Müller, R.; Seton, Maria; Flament, Nicolas

    2015-04-01

    Convection in the Earth's mantle that involves plates at the surfaces gives rise to plate velocities that vary through time and depend on the balance of plate boundary forces, with the present-day providing a snapshot of this ongoing process. However, present-day plate velocities do not capture plate behaviour over geologically representative timeframes and thus cannot be used to evaluate factors limiting plate velocities. Previous studies investigated the effects of continental keels on plate speeds by either using the present-day snapshot or a limited number of reconstructed plate configurations, often leading to conflicting results. For example, an early assumption was that continental keels (especially cratons) were unlikely to impede fast plate motions because India's velocity approached ~20 cm/yr in the Eocene prior to the collision with Eurasia. We employ a modern plate reconstruction approach with evolving global topological plate boundaries for the post-Pangea timeframe (since 200 Ma) to evaluate factors controlling plate velocities. Plate boundary configurations and plate velocities are extracted from the open-source and cross-platform plate reconstruction package GPlates (www.gplates.org) at 1 Myr intervals. For each plate, at each timestep, the area of continental and cratonic lithosphere is calculated to evaluate the effect on plate velocities. Our results support that oceanic plates tend to be 2-3 times faster than plates with large portion of continental plate area, consistent with predictions of numerical models of mantle convection. The fastest plates (~8.5 cm/yr RMS) are dominated by oceanic plate area and high subducting portion of plate perimeter, while the slowest plates (~2.6-2.8 cm/yr RMS) are dominated by continental plate area and bounded by transforms and mid-oceanic ridge segments. Importantly, increasing cratonic fractions (both Proterozoic and Archean lithosphere) significantly impede plate velocities, suggesting that deep continental

  3. High-throughput and automated diagnosis of antimicrobial resistance using a cost-effective cellphone-based micro-plate reader

    NASA Astrophysics Data System (ADS)

    Feng, Steve; Tseng, Derek; di Carlo, Dino; Garner, Omai B.; Ozcan, Aydogan

    2016-12-01

    Routine antimicrobial susceptibility testing (AST) can prevent deaths due to bacteria and reduce the spread of multi-drug-resistance, but cannot be regularly performed in resource-limited-settings due to technological challenges, high-costs, and lack of trained professionals. We demonstrate an automated and cost-effective cellphone-based 96-well microtiter-plate (MTP) reader, capable of performing AST without the need for trained diagnosticians. Our system includes a 3D-printed smartphone attachment that holds and illuminates the MTP using a light-emitting-diode array. An inexpensive optical fiber-array enables the capture of the transmitted light of each well through the smartphone camera. A custom-designed application sends the captured image to a server to automatically determine well-turbidity, with results returned to the smartphone in ~1 minute. We tested this mobile-reader using MTPs prepared with 17 antibiotics targeting Gram-negative bacteria on clinical isolates of Klebsiella pneumoniae, containing highly-resistant antimicrobial profiles. Using 78 patient isolate test-plates, we demonstrated that our mobile-reader meets the FDA-defined AST criteria, with a well-turbidity detection accuracy of 98.21%, minimum-inhibitory-concentration accuracy of 95.12%, and a drug-susceptibility interpretation accuracy of 99.23%, with no very major errors. This mobile-reader could eliminate the need for trained diagnosticians to perform AST, reduce the cost-barrier for routine testing, and assist in spatio-temporal tracking of bacterial resistance.

  4. High-throughput and automated diagnosis of antimicrobial resistance using a cost-effective cellphone-based micro-plate reader

    PubMed Central

    Feng, Steve; Tseng, Derek; Di Carlo, Dino; Garner, Omai B.; Ozcan, Aydogan

    2016-01-01

    Routine antimicrobial susceptibility testing (AST) can prevent deaths due to bacteria and reduce the spread of multi-drug-resistance, but cannot be regularly performed in resource-limited-settings due to technological challenges, high-costs, and lack of trained professionals. We demonstrate an automated and cost-effective cellphone-based 96-well microtiter-plate (MTP) reader, capable of performing AST without the need for trained diagnosticians. Our system includes a 3D-printed smartphone attachment that holds and illuminates the MTP using a light-emitting-diode array. An inexpensive optical fiber-array enables the capture of the transmitted light of each well through the smartphone camera. A custom-designed application sends the captured image to a server to automatically determine well-turbidity, with results returned to the smartphone in ~1 minute. We tested this mobile-reader using MTPs prepared with 17 antibiotics targeting Gram-negative bacteria on clinical isolates of Klebsiella pneumoniae, containing highly-resistant antimicrobial profiles. Using 78 patient isolate test-plates, we demonstrated that our mobile-reader meets the FDA-defined AST criteria, with a well-turbidity detection accuracy of 98.21%, minimum-inhibitory-concentration accuracy of 95.12%, and a drug-susceptibility interpretation accuracy of 99.23%, with no very major errors. This mobile-reader could eliminate the need for trained diagnosticians to perform AST, reduce the cost-barrier for routine testing, and assist in spatio-temporal tracking of bacterial resistance. PMID:27976700

  5. Multiple sulfur isotope systematics of Icelandic geothermal fluids and the source and reactions of sulfur in volcanic geothermal systems at divergent plate boundaries

    NASA Astrophysics Data System (ADS)

    Stefánsson, Andri; Keller, Nicole S.; Robin, Jóhann Gunnarsson; Ono, Shuhei

    2015-09-01

    Multiple sulfur isotope systematics of geothermal fluids at Krafla, Northeast Iceland, were studied in order to determine the source and reactions of sulfur in this system, as an example of a geothermal system hosted on a divergent plate boundary. Fluid temperatures ranged from 192 to 437 °C, and the fluids have low Cl concentration between ∼10 and ∼150 ppm, with liquid water and vapor being present in the reservoir. Dissolved sulfide (S-II) and sulfate (SVI) predominated in the water phase with trace concentrations of thiosulfate (S2O32-) whereas sulfide (S-II) was the only species observed in the vapor phase. The reconstructed sulfur isotope ratios of the reservoir fluids based on samples collected at surface from two-phase and vapor only well discharges indicated that δ34S and Δ33S of sulfide in the reservoir fluid ranged from -1.5 to +1.1‰ and -0.001 to -0.017‰, respectively, whereas δ34S and Δ33S of sulfate were significantly different and ranged from +3.4 to +13.4‰ and 0.000 to -0.036‰, respectively. Depressurization boiling upon fluid ascent coupled with progressive fluid-rock interaction and sulfide mineral (pyrite) formation results in the liquid phase becoming progressively isotopically lighter with respect to both δ34S and Δ33S. In contrast, H2S in the vapor phase and pyrite become isotopically heavier. The observed Δ33S and δ34S systematics for geothermal fluids at Krafla suggest that the source of sulfide in the reservoir fluids is the basaltic magma, either through degassing or upon dissolution of unaltered basalts. At high temperatures, insignificant SO4 was observed in the fluids but below ∼230 °C significant concentrations of SO4 were observed, the source inferred to be H2S oxidation. The two key factors controlling the multiple sulfur isotope systematics of geothermal fluids are: (1) the isotopic composition of the source material and (2) the isotope fractionation associated with aqueous and vapor speciation and how these

  6. Reduced Plating Ignitron

    NASA Technical Reports Server (NTRS)

    Polzin, Kurt A (Inventor); Pearson, J Boise (Inventor)

    2014-01-01

    An ignitron apparatus has an airtight tubular housing having a first sealed end and a second sealed end. An anode is connected at the first sealed end, projecting into the housing, and a recess at the second sealed and forms a well which contains a quantity of liquid gallium or gallium alloy making up the cathode. An ignitor projects through the liquid metal and into the housing. The inner surface of the housing includes at least one plating-reduction structure to prevent electrical shorting of the apparatus caused by plating of the liquid metal.

  7. NICKEL PLATING PROCESS

    DOEpatents

    Hoover, T.B.; Zava, T.E.

    1959-05-12

    A simplified process is presented for plating nickel by the vapor decomposition of nickel carbonyl. In a preferred form of the invention a solid surface is nickel plated by subjecting the surface to contact with a mixture containing by volume approximately 20% nickel carbonyl vapor, 2% hydrogen sulfide and .l% water vapor or 1% oxygen and the remainder carbon dioxide at room temperature until the desired thickness of nickel is obtained. The advantage of this composition over others is that the normally explosive nickel carbonyl is greatly stabilized.

  8. License plate detection algorithm

    NASA Astrophysics Data System (ADS)

    Broitman, Michael; Klopovsky, Yuri; Silinskis, Normunds

    2013-12-01

    A novel algorithm for vehicle license plates localization is proposed. The algorithm is based on pixel intensity transition gradient analysis. Near to 2500 natural-scene gray-level vehicle images of different backgrounds and ambient illumination was tested. The best set of algorithm's parameters produces detection rate up to 0.94. Taking into account abnormal camera location during our tests and therefore geometrical distortion and troubles from trees this result could be considered as passable. Correlation between source data, such as license Plate dimensions and texture, cameras location and others, and parameters of algorithm were also defined.

  9. Cascade impactor and jet plate for same

    DOEpatents

    Dahlin, Robert S.; Farthing, William E.; Landham Jr., Edward C.

    2004-02-03

    A sampling system and method for sampling particulate matter from a high-temperature, high-pressure gas stream. A cyclone sampler for use at high temperatures and pressures, and having threadless sacrificial connectors is disclosed. Also disclosed is an improved cascade impactor including jet plates with integral spacers, and alignment features provided for aligning the jet plates with their associated collection substrates. An activated bauxite alkali collector is disclosed, and includes an alumina liner. The sampling system can be operated remotely or locally, and can be permanently installed or configured as a portable system.

  10. Ponderomotive phase plate for transmission electron microscopes

    DOEpatents

    Reed, Bryan W [Livermore, CA

    2012-07-10

    A ponderomotive phase plate system and method for controllably producing highly tunable phase contrast transfer functions in a transmission electron microscope (TEM) for high resolution and biological phase contrast imaging. The system and method includes a laser source and a beam transport system to produce a focused laser crossover as a phase plate, so that a ponderomotive potential of the focused laser crossover produces a scattering-angle-dependent phase shift in the electrons of the post-sample electron beam corresponding to a desired phase contrast transfer function.

  11. Robot Arm with Tendon Connector Plate and Linear Actuator

    NASA Technical Reports Server (NTRS)

    Ihrke, Chris A. (Inventor); Diftler, Myron A. (Inventor); Bridgwater, Lyndon (Inventor); Nguyen, Vienny (Inventor); Millerman, Alexander (Inventor)

    2014-01-01

    A robotic system includes a tendon-driven end effector, a linear actuator, a flexible tendon, and a plate assembly. The linear actuator assembly has a servo motor and a drive mechanism, the latter of which translates linearly with respect to a drive axis of the servo motor in response to output torque from the servo motor. The tendon connects to the end effector and drive mechanism. The plate assembly is disposed between the linear actuator assembly and the tendon-driven end effector and includes first and second plates. The first plate has a first side that defines a boss with a center opening. The second plate defines an accurate through-slot having tendon guide channels. The first plate defines a through passage for the tendon between the center opening and a second side of the first plate. A looped end of the flexible tendon is received within the tendon guide channels.

  12. Intermittent Plate Tectonics

    NASA Astrophysics Data System (ADS)

    Silver, P. G.; Behn, M. D.

    2006-12-01

    Intermittent Plate Tectonics A basic premise of Earth Science is that plate tectonics has been continuously operating since it began early in Earth's history. Yet, plate-tectonic theory itself, specifically the collisional phase of the Wilson Cycle, constitutes a process that is capable of stopping all plate motion. The plausibility of a plate-tectonic hiatus is most easily illustrated by considering the expected future of the present-day plate-tectonic configuration. Since the opening of the Atlantic at ~200 ma, the area of the Atlantic basin has been growing at the expense of the Pacific. If this trend continues, relative plate motion models predict that in ~350 my, the Pacific Ocean basin will effectively close leading to widespread continent-continent collisions. Since a continent-continent collision represents the termination of subduction locally, the accumulated effect of all collisions is to stop subduction globally. In this scenario, ridges would then stop spreading and young oceanic lithosphere would cool, reaching a steady-state thickness of 100 km in about 80 my, based on the properties of oceanic lithosphere today. This would constitute the stoppage of plate tectonics. The presumption that plate tectonics never stops in the face of continental collisions is equivalent to requiring that subduction flux is approximately constant through time, such that subduction initiation roughly balances subduction termination. Such a balance then raises several questions about the subduction initiation process. When and how does subduction initiate? Is there a detectible relationship between subduction cessation and subduction initiation? We can gain some guidance into these questions by examining the plate motion history over the last 200 my. Subduction initiation has occurred over the last 80 my in three intra- oceanic subduction zones: Aleutians, Marianas-Izu-Bonin and Tonga-Kermadec in the Pacific basin. In these cases, however, subduction initiation would not

  13. Nuclear reactor alignment plate configuration

    SciTech Connect

    Altman, David A; Forsyth, David R; Smith, Richard E; Singleton, Norman R

    2014-01-28

    An alignment plate that is attached to a core barrel of a pressurized water reactor and fits within slots within a top plate of a lower core shroud and upper core plate to maintain lateral alignment of the reactor internals. The alignment plate is connected to the core barrel through two vertically-spaced dowel pins that extend from the outside surface of the core barrel through a reinforcement pad and into corresponding holes in the alignment plate. Additionally, threaded fasteners are inserted around the perimeter of the reinforcement pad and into the alignment plate to further secure the alignment plate to the core barrel. A fillet weld also is deposited around the perimeter of the reinforcement pad. To accomodate thermal growth between the alignment plate and the core barrel, a gap is left above, below and at both sides of one of the dowel pins in the alignment plate holes through with the dowel pins pass.

  14. Polar Plate Theory for Orthogonal Anisotropy

    NASA Technical Reports Server (NTRS)

    Bailey, Michelle D.; Bower, Mark V.

    2000-01-01

    Laminated fiber-reinforced (or filamentary) composites are used today for their high strength-to-weight and stiffness-to-weight ratios. However, because of the anisotropic behavior of composites, determining the response on a macroscopic scale is challenging. This is particularly evident in the evaluation of the governing differential equations of a circular disk with the fibers of the lamina oriented with rectilinear orthogonality. This includes any situation involving a composite plate of circular geometry in which out-of-plane displacements due to load are desired, such as fastener pull through loading of a composite plate. Current analysis techniques use numerical methods with rectilinear coordinate systems to solve problems with circular geometry. These analyses over predict plate stiffness by 20% and underpredict failure by 70%. Consequently, there is a need to transform classical composite plate theory to a polar coordinate system. In order to better analyze structures with circular geometries the classical composite plate equations are transformed into the plate equations for a rectilinearly anisotropic composite in polar coordinates. A composite plate is typically a laminate of fibers in rectilinear directions. Subsequent to the lay-tip the necessary geometry is cut out of a rectangular plate. In a similar manner, the derivation of the plate equation starts with the fundamental definitions of strain, displacement and curvature and incorporates the material property angular dependence into the equilibrium equations for a differential polar element. In the transformed state, the stiffness coefficients are no longer constant, adding to the complexity of the governing differential equations. This paper discusses the new derivation and evaluation of the plate equations for a circular composite disk with orthogonal rectilinear anisotropy. The resultant new three partial differential equations, which describe the circular anisotropic plate, can be used to

  15. The Plate Tectonics Project

    ERIC Educational Resources Information Center

    Hein, Annamae J.

    2011-01-01

    The Plate Tectonics Project is a multiday, inquiry-based unit that facilitates students as self-motivated learners. Reliable Web sites are offered to assist with lessons, and a summative rubric is used to facilitate the holistic nature of the project. After each topic (parts of the Earth, continental drift, etc.) is covered, the students will…

  16. Unitary plate electrode

    NASA Technical Reports Server (NTRS)

    Rowlette, John J. (Inventor); Clough, Thomas J. (Inventor); Josefowicz, Jack Y. (Inventor); Sibert, John W. (Inventor)

    1985-01-01

    The unitary electrode (10) comprises a porous sheet (12) of fiberglass the strands (14) of which contain a coating (16) of conductive tin oxide. The lower portion of the sheet contains a layer (18) of resin and the upper layer (20) contains lead dioxide forming a positive active electrode on an electrolyte-impervious layer. The strands (14) form a continuous conduction path through both layers (16, 18). Tin oxide is prevented from reduction by coating the surface of the plate facing the negative electrode with a conductive, impervious layer resistant to reduction such as a thin film (130) of lead or graphite filled resin adhered to the plate with a layer (31) of conductive adhesive. The plate (10) can be formed by casting a molten resin from kettle (60) onto a sheet of glass wool (56) overlying a sheet of lead foil and then applying positive active paste from hopper (64) into the upper layer (68). The plate can also be formed by passing an assembly of a sheet ( 80) of resin, a sheet (86) of sintered glass and a sheet (90) of lead between the nip (92) of heated rollers (93, 95) and then filling lead oxide into the pores (116) of the upper layer (118).

  17. Growth Plate Injuries

    MedlinePlus

    ... or crushed, the growth plate may close prematurely, forming a bony bridge or “bar.” The risk of ... this publication: James S. Panagis, M.D., M.P.H., NIAMS/NIH; R. Tracy Ballock, M.D., Case ...

  18. INL HIP Plate Fabrication

    SciTech Connect

    B. H. Park; C. R. Clark; J. F. Jue

    2010-02-01

    This document outlines the process used to bond monolithic fuel plates by Hot Isostatic Pressing (HIP). This method was developed at Idaho National Laboratory (INL) for the Reduced Enrichment for Research and Test Reactors (RERTR) program. These foils have been used in a number of irradiation experiments in support of the United States Global Threat Reduction Initiative (GTRI) program.

  19. Congruency of scapula locking plates: implications for implant design.

    PubMed

    Park, Andrew Y; DiStefano, James G; Nguyen, Thuc-Quyen; Buckley, Jenni M; Montgomery, William H; Grimsrud, Chris D

    2012-04-01

    We conducted a study to evaluate the congruency of fit of current scapular plate designs. Three-dimensional image-processing and -analysis software, and computed tomography scans of 12 cadaveric scapulae were used to generate 3 measurements: mean distance from plate to bone, maximum distance, and percentage of plate surface within 2 mm of bone. These measurements were used to quantify congruency. The scapular spine plate had the most congruent fit in all 3 measured variables. The lateral border and glenoid plates performed statistically as well as the scapular spine plate in at least 1 of the measured variables. The medial border plate had the least optimal measurements in all 3 variables. With locking-plate technology used in a wide variety of anatomical locations, the locking scapula plate system can allow for a fixed-angle construct in this region. Our study results showed that the scapular spine, glenoid, and lateral border plates are adequate in terms of congruency. However, design improvements may be necessary for the medial border plate. In addition, we describe a novel method for quantifying hardware congruency, a method that can be applied to any anatomical location.

  20. Analysis of boiling flat-plate collectors

    SciTech Connect

    Price, H.W.; Klein, S.A.; Beckman, W.A.

    1986-05-01

    A detailed model for use with TRNSYS, capable of modelling a wide range of boiling collector types, was used to analyze boiling flat-plate collector systems. This model can account for a subcooled liquid entering the collector, heat losses in the vapor and the liquid return line, pressure drops due to friction in the collector and piping, and pressure drops due to the hydrostatic head of the fluid. The model has been used to determine the yearly performance of boiling flat-plate solar collector systems. A simplified approach was also developed which can be used with the f-Chart method to predict yearly performance of boiling flat-plate collector systems.

  1. Dermatitis from a chromium dental plate.

    PubMed

    Hubler, W R; Hubler, W R

    1983-09-01

    Systemic absorption of metal or metallic salts from dental and orthopedic surgical implants can produce a cutaneous allergic dermatitis in susceptible individuals. Mercury, nickel and cobalt are the most common metals to elicit such systemic allergic reactions from chronic internal exposure. A case is presented of a generalized eczematoid dermatitis apparently caused by allergy to chromium liberated from a metal dental plate.

  2. Uplift of Zagros Mountains slows plate convergence

    NASA Astrophysics Data System (ADS)

    Balcerak, Ernie

    2013-05-01

    Research has indicated that mountain ranges can slow down the convergence between two tectonic plates on timescales as short as a few million years, as the growing mountains provide enough tectonic force to impact plate motions. Focusing on the convergence of the Arabian and Eurasian plates at the Zagros mountain range, which runs across Iran and Iraq, Austermann and Iaffaldano reconstructed the relative motion of the plates using published paleomagnetic data covering the past 13 million years, as well as current geodetic measurements. They show that the convergence of the two plates has decreased by about 30% over the past 5 million years. Looking at the geological record to infer past topography and using a computer model of the mantle-lithosphere system, the authors examined whether the recent uplift across the Zagros Mountains could have caused the observed slowdown. They also considered several other geological events that might have influenced the convergence rate, but the authors were able to rule those out as dominant controls. The authors conclude that the uplift across the Zagros Mountains in the past 5 million years did indeed play a key role in slowing down the convergence between the Eurasian and Arabian plates. (Tectonics, doi:10.1002/tect.20027, 2013)

  3. Models for rupture mechanics of plate boundaries and crustal deformation

    NASA Astrophysics Data System (ADS)

    Nur, A.

    1983-02-01

    The role of pull aparts and pushups in transcurrent systems, the rotation of faults and blocks within transcurrent fault systems, the role of accretion tectonics in plate boundary deformation, and power law creep behavior and the yielding at plate boundaries were investigated.

  4. Models for rupture mechanics of plate boundaries and crustal deformation

    NASA Technical Reports Server (NTRS)

    Nur, A.

    1983-01-01

    The role of pull aparts and pushups in transcurrent systems, the rotation of faults and blocks within transcurrent fault systems, the role of accretion tectonics in plate boundary deformation, and power law creep behavior and the yielding at plate boundaries were investigated.

  5. Principal visual word discovery for automatic license plate detection.

    PubMed

    Zhou, Wengang; Li, Houqiang; Lu, Yijuan; Tian, Qi

    2012-09-01

    License plates detection is widely considered a solved problem, with many systems already in operation. However, the existing algorithms or systems work well only under some controlled conditions. There are still many challenges for license plate detection in an open environment, such as various observation angles, background clutter, scale changes, multiple plates, uneven illumination, and so on. In this paper, we propose a novel scheme to automatically locate license plates by principal visual word (PVW), discovery and local feature matching. Observing that characters in different license plates are duplicates of each other, we bring in the idea of using the bag-of-words (BoW) model popularly applied in partial-duplicate image search. Unlike the classic BoW model, for each plate character, we automatically discover the PVW characterized with geometric context. Given a new image, the license plates are extracted by matching local features with PVW. Besides license plate detection, our approach can also be extended to the detection of logos and trademarks. Due to the invariance virtue of scale-invariant feature transform feature, our method can adaptively deal with various changes in the license plates, such as rotation, scaling, illumination, etc. Promising results of the proposed approach are demonstrated with an experimental study in license plate detection.

  6. This-layer chromatography/electrospray ionization triple-quadrupole linear ion trap mass spectrometry system: analysis of rhodamine dyes separated on reversed-phase C8 plates

    SciTech Connect

    Ford, Michael J; Kertesz, Vilmos; Van Berkel, Gary J

    2005-01-01

    The direct analysis of separated rhodamine dyes on reversed-phase C{sub 8} thin-layer chromatography plates using a surface sampling/electrospray emitter probe coupled with a triple-quadrupole linear ion trap mass spectrometer is presented. This report represents continuing work to advance the performance metrics and utility of this basic surface sampling electrospray mass spectrometry system for the analysis of thin-layer chromatography plates. Experimental results examining the role of sampling probe spray end configuration on liquid aspiration rate and gas-phase ion signal generated are discussed. The detection figures-of-merit afforded by full-scan, automated product ion and selected reaction monitoring modes of operation were examined. The effect of different eluting solvents on mass spectrum signal levels with the reversed-phase C{sub 8} plate was investigated. The combined effect of eluting solvent flow-rate and development lane surface scan rate on preservation of chromatographic resolution was also studied. Analysis of chromatographically separated red pen ink extracts from eight different pens using selected reaction monitoring demonstrated the potential of this surface sampling electrospray mass spectrometry system for targeted compound analysis with real samples.

  7. Design analysis for a nutating plate drive 4

    NASA Technical Reports Server (NTRS)

    Loewenthal, S. H.; Townsend, D. P.

    1972-01-01

    A simplified design analysis was conducted on a nutating plate type drive system for a 2500 horsepower helicopter main rotor gear box. A drive system that split the output torque evenly between two nutating plates for the purpose of reducing the load on each nutating plate was analyzed. Needle bearings were used on the nutating plate pins. The results of the analysis indicate that the required load capacity of the pin bearings and the speed of the nutating plate bearings were beyond the state-of-the-art capacity of rolling-element bearings. The analysis further indicates that the nutating plate drive is less efficient, and results in a higher weight per horsepower than a conventional planetary helicopter transmission with similar design specifications.

  8. What Are Growth Plate Injuries?

    MedlinePlus

    ... nih.gov November 2014 What Are Growth Plate Injuries? Fast Facts: An Easy-to-Read Series of Publications ... Some inherited disorders 1 What Are Growth Plate Injuries? Fast Facts: An Easy-to-Read Series of Publications ...

  9. Large Deflections of Elastic Rectangular Plates

    NASA Astrophysics Data System (ADS)

    Razdolsky, A. G.

    2015-11-01

    It is known that elastic large deflections of thin plates are governed by von Karman nonlinear equations. The analytical solution of these equations in the general case is unfeasible. Samuel Levy, in 1942, showed that large deflections of the rectangular plate can be expressed as a double series of sine-shaped harmonics (deflection harmonics). However, this method gave no way of creating the computer algorithm of solving the problem. The stress function expression taken in the Levy's method must be revised to find the approach that takes into account of all possible products of deflection coefficients. The algorithm of solving the problem for the rectangular plate with an arbitrary aspect ratio under the action of the lateral distributed load is reported in this paper. The approximation of the plate deflection is taken in the form of double series proposed by Samuel Levy. However, the expression for the stress function is presented in the form that incorporates products of deflection coefficients in the explicit form in distinction to the Levy's expression. The number of harmonics in the deflection expression may be arbitrary. The algorithm provides composing the system of governing cubic equations, which includes the deflection coefficients in the explicit form. Solving the equation system is based on using the principle of minimum potential energy. A method of the gradient descent is applied to find the equilibrium state of the plate as the minimum point of the potential energy. A computer program is developed on the basis of the present algorithm. Numerical examples carried out for the plate model with 16 deflection harmonics illustrate the potentialities of the program. The results of solving the examples are presented in the graphical form for the plates with a different aspect ratio and may be used under designing thin-walled elements of airplane and ship structures.

  10. Strain Library Imaging Protocol for high-throughput, automated single-cell microscopy of large bacterial collections arrayed on multiwell plates.

    PubMed

    Shi, Handuo; Colavin, Alexandre; Lee, Timothy K; Huang, Kerwyn Casey

    2017-02-01

    Single-cell microscopy is a powerful tool for studying gene functions using strain libraries, but it suffers from throughput limitations. Here we describe the Strain Library Imaging Protocol (SLIP), which is a high-throughput, automated microscopy workflow for large strain collections that requires minimal user involvement. SLIP involves transferring arrayed bacterial cultures from multiwell plates onto large agar pads using inexpensive replicator pins and automatically imaging the resulting single cells. The acquired images are subsequently reviewed and analyzed by custom MATLAB scripts that segment single-cell contours and extract quantitative metrics. SLIP yields rich data sets on cell morphology and gene expression that illustrate the function of certain genes and the connections among strains in a library. For a library arrayed on 96-well plates, image acquisition can be completed within 4 min per plate.

  11. Computational models for the nonlinear analysis of reinforced concrete plates

    NASA Technical Reports Server (NTRS)

    Hinton, E.; Rahman, H. H. A.; Huq, M. M.

    1980-01-01

    A finite element computational model for the nonlinear analysis of reinforced concrete solid, stiffened and cellular plates is briefly outlined. Typically, Mindlin elements are used to model the plates whereas eccentric Timoshenko elements are adopted to represent the beams. The layering technique, common in the analysis of reinforced concrete flexural systems, is incorporated in the model. The proposed model provides an inexpensive and reasonably accurate approach which can be extended for use with voided plates.

  12. Microchannel plate streak camera

    DOEpatents

    Wang, Ching L.

    1989-01-01

    An improved streak camera in which a microchannel plate electron multiplier is used in place of or in combination with the photocathode used in prior streak cameras. The improved streak camera is far more sensitive to photons (UV to gamma-rays) than the conventional x-ray streak camera which uses a photocathode. The improved streak camera offers gamma-ray detection with high temporal resolution. It also offers low-energy x-ray detection without attenuation inside the cathode. Using the microchannel plate in the improved camera has resulted in a time resolution of about 150 ps, and has provided a sensitivity sufficient for 1000 KeV x-rays.

  13. Bipolar battery plate

    NASA Technical Reports Server (NTRS)

    Rowlette, John J. (Inventor)

    1985-01-01

    A liquid-impermeable plate (10) having throughplate conductivity with essentially zero resistance comprises an insulator sheet (12) having a series of spaced perforations (14) each of which contains a metal element (16) sealingly received into the perforation (14). A low-cost plate can readily be manufactured by punching a thermoplastic sheet (40) such as polypropylene with a punching tool (52), filling the apertures with lead spheres (63) having a diameter smaller than the holes (50) but larger than the thickness of the sheet, sweeping excess spheres (62) off the sheet with a doctor blade (60) and then pressing a heated platen (74) onto the sheet to swage the spheres into a cylindrical shape and melt the surrounding resin to form a liquid-impermeable collar (4) sealing the metal into the sheet.

  14. Microchannel plate streak camera

    DOEpatents

    Wang, C.L.

    1984-09-28

    An improved streak camera in which a microchannel plate electron multiplier is used in place of or in combination with the photocathode used in prior streak cameras. The improved streak camera is far more sensitive to photons (uv to gamma-rays) than the conventional x-ray streak camera which uses a photocathode. The improved streak camera offers gamma-ray detection with high temporal resolution. It also offers low-energy x-ray detection without attenuation inside the cathode. Using the microchannel plate in the improved camera has resulted in a time resolution of about 150 ps, and has provided a sensitivity sufficient for 1000 keV x-rays.

  15. Microchannel plate streak camera

    DOEpatents

    Wang, C.L.

    1989-03-21

    An improved streak camera in which a microchannel plate electron multiplier is used in place of or in combination with the photocathode used in prior streak cameras is disclosed. The improved streak camera is far more sensitive to photons (UV to gamma-rays) than the conventional x-ray streak camera which uses a photocathode. The improved streak camera offers gamma-ray detection with high temporal resolution. It also offers low-energy x-ray detection without attenuation inside the cathode. Using the microchannel plate in the improved camera has resulted in a time resolution of about 150 ps, and has provided a sensitivity sufficient for 1,000 KeV x-rays. 3 figs.

  16. Mechanisms in Thermal Mechanical Forming of Plates.

    DTIC Science & Technology

    1987-05-01

    specific locations within the plate is discussed, and recommendations for further research are made. Keywords: Metal plates; Ship plates; Material forming; Thermomechanics; Edge effect ; Laser line heating.

  17. Robotic production of cancer cell spheroids with an aqueous two-phase system for drug testing.

    PubMed

    Ham, Stephanie Lemmo; Atefi, Ehsan; Fyffe, Darcy; Tavana, Hossein

    2015-04-23

    Cancer cell spheroids present a relevant in vitro model of avascular tumors for anti-cancer drug testing applications. A detailed protocol for producing both mono-culture and co-culture spheroids in a high throughput 96-well plate format is described in this work. This approach utilizes an aqueous two-phase system to confine cells into a drop of the denser aqueous phase immersed within the second aqueous phase. The drop rests on the well surface and keeps cells in close proximity to form a single spheroid. This technology has been adapted to a robotic liquid handler to produce size-controlled spheroids and expedite the process of spheroid production for compound screening applications. Spheroids treated with a clinically-used drug show reduced cell viability with increase in the drug dose. The use of a standard micro-well plate for spheroid generation makes it straightforward to analyze viability of cancer cells of drug-treated spheroids with a micro-plate reader. This technology is straightforward to implement both robotically and with other liquid handling tools such as manual pipettes.

  18. Plated wire memory subsystem

    NASA Technical Reports Server (NTRS)

    Reynolds, L.; Tweed, H.

    1972-01-01

    The work performed entailed the design, development, construction and testing of a 4000 word by 18 bit random access, NDRO plated wire memory for use in conjunction with a spacecraft imput/output unit and central processing unit. The primary design parameters, in order of importance, were high reliability, low power, volume and weight. A single memory unit, referred to as a qualification model, was delivered.

  19. Elastic plate spallation

    NASA Technical Reports Server (NTRS)

    Oline, L.; Medaglia, J.

    1972-01-01

    The dynamic finite element method was used to investigate elastic stress waves in a plate. Strain displacement and stress strain relations are discussed along with the stiffness and mass matrix. The results of studying point load, and distributed load over small, intermediate, and large radii are reported. The derivation of finite element matrices, and the derivation of lumped and consistent matrices for one dimensional problems with Laplace transfer solutions are included. The computer program JMMSPALL is also included.

  20. Martian plate tectonics

    NASA Astrophysics Data System (ADS)

    Sleep, N. H.

    1994-03-01

    The northern lowlands of Mars have been produced by plate tectonics. Preexisting old thick highland crust was subducted, while seafloor spreading produced thin lowland crust during late Noachian and Early Hesperian time. In the preferred reconstruction, a breakup margin extended north of Cimmeria Terra between Daedalia Planum and Isidis Planitia where the highland-lowland transition is relatively simple. South dipping subduction occured beneath Arabia Terra and east dipping subduction beneath Tharsis Montes and Tempe Terra. Lineations associated with Gordii Dorsum are attributed to ridge-parallel structures, while Phelegra Montes and Scandia Colles are interpreted as transfer-parallel structures or ridge-fault-fault triple junction tracks. Other than for these few features, there is little topographic roughness in the lowlands. Seafloor spreading, if it occurred, must have been relatively rapid. Quantitative estimates of spreading rate are obtained by considering the physics of seafloor spreading in the lower (approx. 0.4 g) gravity of Mars, the absence of vertical scarps from age differences across fracture zones, and the smooth axial topography. Crustal thickness at a given potential temperature in the mantle source region scales inversely with gravity. Thus, the velocity of the rough-smooth transition for axial topography also scales inversely with gravity. Plate reorganizations where young crust becomes difficult to subduct are another constraint on spreading age. Plate tectonics, if it occurred, dominated the thermal and stress history of the planet. A geochemical implication is that the lower gravity of Mars allows deeper hydrothermal circulation through cracks and hence more hydration of oceanic crust so that more water is easily subducted than on the Earth. Age and structural relationships from photogeology as well as median wavelength gravity anomalies across the now dead breakup and subduction margins are the data most likely to test and modify hypotheses

  1. Electronic Equipment Cold Plates

    DTIC Science & Technology

    1976-04-01

    equations for such a flow regiae. For laainar flow and Moderate teaperature differwwe« between the well «nd coolant, a aodifled Sieder -Tate...con- figuration. The heat-transfer coefficients, therefore, were determined by using both the Sieder -Tate and McAdams equations and the coaputed...values used In the analytical predictions. As with th* previous cold Plates, the Sieder -Tate equation gave too low of values for the heat- transfer

  2. The Plate Overlap Technique.

    DTIC Science & Technology

    1978-07-31

    INTRODUCTION 1 II. NOTATION 2 III. THE GNOMONIC PROJECTION 4 IV . THE PLATE OVERLAP TECHNIQUE 6 A. MOTIVATION 6 B. FORNULATION 9 C. ON STATISTICAL RIGOR 14 D...and new hardware. Since this aim was clearly recognized long ago, wherever possible in earlier documents or software development flexibility was...reader should see 1, 2, and 3. The procedures one should use to update stellar positions are discussed in 4 with applica- tions to the SAOC in 5. Non

  3. Plate motion and deformation

    SciTech Connect

    Minster, B.; Prescott, W.; Royden, L.

    1991-02-01

    Our goal is to understand the motions of the plates, the deformation along their boundaries and within their interiors, and the processes that control these tectonic phenomena. In the broadest terms, we must strive to understand the relationships of regional and local deformation to flow in the upper mantle and the rheological, thermal and density structure of the lithosphere. The essential data sets which we require to reach our goal consist of maps of current strain rates at the earth's surface and the distribution of integrated deformation through time as recorded in the geologic record. Our success will depend on the effective synthesis of crustal kinematics with a variety of other geological and geophysical data, within a quantitative theoretical framework describing processes in the earth's interior. Only in this way can we relate the snapshot of current motions and earth structure provided by geodetic and geophysical data with long-term processes operating on the time scales relevant to most geological processes. The wide-spread use of space-based techniques, coupled with traditional geological and geophysical data, promises a revolution in our understanding of the kinematics and dynamics of plate motions over a broad range of spatial and temporal scales and in a variety of geologic settings. The space-based techniques that best address problems in plate motion and deformation are precise space-geodetic positioning -- on land and on the seafloor -- and satellite acquisition of detailed altimetric and remote sensing data in oceanic and continental areas. The overall science objectives for the NASA Solid Earth Science plan for the 1990's, are to Understand the motion and deformation of the lithosphere within and across plate boundaries'', and to understand the dynamics of the mantle, the structure and evolution of the lithosphere, and the landforms that result from local and regional deformation. 57 refs., 7 figs., 2 tabs.

  4. Armor Plate Surface Roughness Measurements

    DTIC Science & Technology

    2005-04-01

    Armor Plate Surface Roughness Measurements by Brian Stanton, William Coburn, and Thomas J. Pizzillo ARL-TR-3498 April 2005... Armor Plate Surface Roughness Measurements Brian Stanton, William Coburn and Thomas J. Pizzillo Sensors and Electron Devices Directorate...October 2004 5a. CONTRACT NUMBER 5b. GRANT NUMBER 4. TITLE AND SUBTITLE Armor Plate Surface Roughness Measurements 5c. PROGRAM ELEMENT NUMBER

  5. The Hydrothermal System at Home Plate in Gusev Crater, Mars: Formation of High Silica Material by Acid-Sulfate Alteration of Basalt

    NASA Technical Reports Server (NTRS)

    Morris, R. V.; Ming, D. W.; Gellert, R.; Yen, A.; Clark, B. C.; Gnaff, T. G.; Arvidson, R. E.; Squyres, S. W.

    2008-01-01

    The Alpha Particle X-ray Spectrometer (APXS) instrument on the Mars Exploration Rover (MER) Spirit measured three targets on or adjacent to Home Plate in Gusev Crater that have unusually high SiO2 concentrations (68% to 91%), unusually low FeO concentrations (1% to 7%, with total Fe as FeO), and unusually high TiO2/FeO ratios (0.2 to 1.2 by weight) [1]. Two targets (Kenosha Comets and Lefty Ganote) are located on high albedo soil (Gertrude Weise) that was exposed by the rover wheels, and one target is a float rock called Fuzzy Smith. Kenosha Comets has the highest SiO2 concentration, lowest FeO concentration, and highest TiO2/FeO ratio. Mineralogical evidence from the MER Miniature Thermal Emission Spectrometer (Mini-TES) suggests that the SiO2 is present as amorphous (noncrystalline) SiO2 at Gertrude Weise and nearby targets [2,3]. Mini-TES data were not acquired for Fuzzy Smith. Home Plate is considered to have an explosive volcanic origin, resulting when basaltic magma came into contact with ground water or ice [4]. Within 50 m to 1 km of Home Plate are sulfate rich soil deposits (Paso Robles class soils with 22-35% SO3) which are considered to be probable fumarolic and/or hydrothermal deposits associated with the volcanism [5]. We develop the model here, suggested by [5], that the high-silica materials are another manifestation of acid-sulfate processes associated with fumarolic and hydrothermal activity at Home Plate. This is done by analogy with basaltic materials altered by acid sulfate processes on the Island of Hawaii.

  6. [Chronic non-specific disease of the respiratory system and measurable alterations in workers in the chromium-plating industry (author's transl)].

    PubMed

    Cavatorta, A; Mutti, A; Frigeri, G; Falzoi, M; Cigala, F; Franchini, I

    1980-01-01

    Lung function test performed on 26 chromium plating workers showed a high prevalence of obstructive disease and of change in DLCO. The connection between lung function impairment and occupational exposure to chromium is suggested by the relationship between respiratory changes and biological indices of chromium absorption. The prevalence and the severity of ventilatory impairment also show an increasing trend with the duration of the exposure, while the smoking habitus seems not to be important.

  7. Origin of Small Tectonic Plates

    NASA Astrophysics Data System (ADS)

    Mallard, C.; Coltice, N.; Seton, M.; Müller, D.; Tackley, P.

    2015-12-01

    The plate tectonic theory allowed to split the Earth surface into 6 (Le Pichon 1968) to 52 tectonic plates (Bird 2003). These plates are separated into two groups: the first of 7 large plates and the second of numerous smaller plates (Morra et al 2013). Previous studies using the reconstruction of the past 200 My, suggest that the size of large plates is driven by mantle flow. But the tools employed are descriptive (Morra et al 2013, Sornette and Pisarenko 2003), hence ignoring forces and physical principles within the lithosphere and the mantle. The processes at the origin of small plates remain unknown. We developed a new approach to explain the plate sizes. We demonstrate that the physics of convection drives it. We applied plate tectonics theory on 3D spherical convection models generating plate-like motions, which give access to a complete survey of data: velocities, viscosity and heat flow. Our data show that (1) the large plates depend on the dominating scale of the convective flow due to the initiation or the shutdown of subductions; (2) the smaller plates are generated thanks to large variability of regional stresses along subduction zone by slab pull and suction influenced by the geometry of trenches. Our results are consistent with the quick reorganizations of back-arc basins occuring synchronously with the modification of subduction zones geometry around the Pacific plate (Sdrolias et al 2004). Hence, we conclude that (1) the decreasing number of small plates in the plate reconstructions back in time is an artifact induced by their short lifetime, that is why they are artificially ignored; (2) the geometry of past trenches is simplified leading to an underestimation of the length of subduction zones.

  8. Symmetries in laminated composite plates

    NASA Technical Reports Server (NTRS)

    Noor, A. K.

    1976-01-01

    The different types of symmetry exhibited by laminated anisotropic fibrous composite plates are identified and contrasted with the symmetries of isotropic and homogeneous orthotropic plates. The effects of variations in the fiber orientation and the stacking sequence of the layers on the symmetries exhibited by composite plates are discussed. Both the linear and geometrically nonlinear responses of the plates are considered. A simple procedure is presented for exploiting the symmetries in the finite element analysis. Examples are given of square, skew and polygonal plates where use of symmetry concepts can significantly reduce the scope and cost of analysis.

  9. Orifice plate for controlling solids flow, methods of use thereof and articles comprising the same

    DOEpatents

    Jukkola, Glen D.; Teigen, Bard C.

    2017-01-31

    Disclosed herein is an orifice plate comprising one or more plates having orifices disposed therein; the orifices being operative to permit the flow of solids from a moving bed heat exchanger to a solids flow control system; where the orifice plate is downstream of a tube bundle of the moving bed heat exchanger and upstream of the solids flow control system and wherein the orifice plate is operative to evenly distribute the flow of solids in the solids flow control system.

  10. Yield Surfaces for Anisotropic Plates

    NASA Astrophysics Data System (ADS)

    Walker, J. D.; Thacker, B. H.

    1999-06-01

    Modern aerospace systems are incorporating composite materials into their structures. Often, the composite materials are anisotropic in their mechanical response due to the geometric layout of fibers. For many years, the failure surfaces of anisotropic materials were thought to be characterizable by a quadratic function in the stress, often referred to as a Tsai-Wu yield surface, or, in a more restrictive form, a Tsai-Hill yield surface. Such a representation does not work for materials that are strong in two directions and weak in one direction, which, unfortunately, is the case of most interest since it represents most composite plates. This paper demonstrates the impossibility of modeling the failure surface with both the Tsai-Wu and Tsai-Hill failure surfaces. We then present a yield surface based on the lemniscate, which is quartic in the stress. This new yield surface addresses the case of strong in two directions and weak in one. Calculations with a fragment impacting a composite plate modeled with the new yield surface are presented. Modifications of the yield surface are presented to allow, in a limited way, materials that are both anisotropic and have differing strengths in tension and compression.

  11. 30 CFR 27.7 - Certification plate or label.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... APPROVAL OF MINING PRODUCTS METHANE-MONITORING SYSTEMS General Provisions § 27.7 Certification plate or label. A certified methane-monitoring system or component thereof shall be identified with...

  12. 30 CFR 27.7 - Certification plate or label.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... APPROVAL OF MINING PRODUCTS METHANE-MONITORING SYSTEMS General Provisions § 27.7 Certification plate or label. A certified methane-monitoring system or component thereof shall be identified with...

  13. Physical optics for oven-plate scattering prediction

    NASA Technical Reports Server (NTRS)

    Baldauf, J.; Lambert, K.

    1991-01-01

    An oven assembly design is described, which will be used to determine the effects of temperature on the electrical properties of materials which are used as coatings for metal plates. Experimentally, these plates will be heated to a very high temperature in the oven assembly, and measured using a microwave reflectance measurement system developed for the NASA Lewis Research Center, Near-Field Facility. One unknown in this measurement is the effect that the oven assembly will have on the reflectance properties of the plate. Since the oven will be much larger than the plate, the effect could potentially be significant as the size of the plate becomes smaller. Therefore, it is necessary to predict the effect of the oven on the measurement of the plate. A method for predicting the oven effect is described, and the theoretical oven effect is compared to experimental results of the oven material. The computer code which is used to predict the oven effect is also described.

  14. Hypervelocity impact on shielded plates

    NASA Technical Reports Server (NTRS)

    Smith, James P.

    1993-01-01

    A ballistic limit equation for hypervelocity impact on thin plates is derived analytically. This equation applies to cases of impulsive impact on a plate that is protected by a multi-shock shield, and it is valid in the range of velocity above 6 km/s. Experimental tests were conducted at the NASA Johnson Space Center on square aluminum plates. Comparing the center deflections of these plates with the theoretical deflections of a rigid-plastic plate subjected to a blast load, one determines the dynamic yield strength of the plate material. The analysis is based on a theory for the expansion of the fragmented projectile and on a simple failure criterion. Curves are presented for the critical projectile radius versus the projectile velocity, and for the critical plate thickness versus the velocity. These curves are in good agreement with curves that have been generated empirically.

  15. Fuel cell end plate structure

    DOEpatents

    Guthrie, Robin J.; Katz, Murray; Schroll, Craig R.

    1991-04-23

    The end plates (16) of a fuel cell stack (12) are formed of a thin membrane. Pressure plates (20) exert compressive load through insulation layers (22, 26) to the membrane. Electrical contact between the end plates (16) and electrodes (50, 58) is maintained without deleterious making and breaking of electrical contacts during thermal transients. The thin end plate (16) under compressive load will not distort with a temperature difference across its thickness. Pressure plate (20) experiences a low thermal transient because it is insulated from the cell. The impact on the end plate of any slight deflection created in the pressure plate by temperature difference is minimized by the resilient pressure pad, in the form of insulation, therebetween.

  16. Characteristics of a microchannel plate intensifier

    NASA Technical Reports Server (NTRS)

    Sandie, W. G.; Mende, S. B.

    1982-01-01

    A telephoto optical system has been designed for imaging applications involving a use of the spectral region from 2000 to 7500 A. The optics images onto the photocathode of a multichannel plate photomultiplier (PMT). Interference filters are employed to provide spectral selectivity. The PMT consists of three microchannel plate intensifiers (MCP), arranged in a 'Z' configuration. MCP photomultiplier pulse height distributions are discussed along with aspects of spatial distribution, the electron gain pulse height distribution, and the charge loss due to edge effects. Attention is given to the electron gain pulse height distribution obtained from four anodes connected in parallel, taking into account a uniform illumination of the photocathode.

  17. Fuel Cell Thermal Management Through Conductive Cooling Plates

    NASA Technical Reports Server (NTRS)

    Colozza, Anthony J.; Burke, Kenneth A.

    2008-01-01

    An analysis was performed to evaluate the concept of utilizing conductive cooling plates to remove heat from a fuel cell stack, as opposed to a conventional internal cooling loop. The potential advantages of this type of cooling system are reduced stack complexity and weight and increased reliability through the reduction of the number of internal fluid seals. The conductive cooling plates would extract heat from the stack transferring it to an external coolant loop. The analysis was performed to determine the required thickness of these plates. The analysis was based on an energy balance between the thermal energy produced within the stack and the heat removal from the cooling plates. To accomplish the energy balance, the heat flow into and along the plates to the cooling fluid was modeled. Results were generated for various numbers of cells being cooled by a single cooling plate. The results provided cooling plate thickness, mass, and operating temperature of the plates. It was determined that utilizing high-conductivity pyrolitic graphite cooling plates can provide a specific cooling capacity (W/kg) equivalent to or potentially greater than a conventional internal cooling loop system.

  18. Rotary sequencing valve with flexible port plate

    DOEpatents

    Wagner, Glenn Paul

    2005-05-10

    Rotary sequencing valve comprising a rotor having a rotor face rotatable about an axis perpendicular to the rotor face, wherein the rotor face has a plurality of openings, one or more of which are disposed at a selected radial distance from the axis, and wherein the rotor includes at least one passage connecting at least one pair of the plurality of openings. The valve includes a flexible port plate having a first side and a second side, wherein the first side faces the rotor and engages the rotor such that the flexible port plate can be rotated coaxially by the rotor and can move axially with respect to the rotor, wherein the flexible port plate has a plurality of ports between the first and second sides, which ports are aligned with the openings in the rotor face. The valve also includes a stator having a stator face disposed coaxially with the rotor and the flexible port plate, wherein the second side of the flexible port plate is in sealable, slidable rotary contact with the stator face, wherein the stator face has a plurality of openings, some of which are disposed at the selected radial distance from the axis, and wherein the plurality of openings extend as passages through the stator. The valve may be used in pressure or temperature swing adsorption systems.

  19. Tectonic development of the Maya plate

    SciTech Connect

    Charleston, S.; Concit, S.C.; Sanchez, R.

    1985-01-01

    The Maya Plate is located at the southern margin of the North American Plate, it comprises from north to south, the following tectonic provinces: The Yucatan Platform, (including the lowlands of Peten and the oil-rich offshore platform of the Campeche Bank), the Macuspana basin, the Salt Basin, the NW-SE trending Sierra de Chiapas Folded Belt, the Chiapas depression and the Ciapas Massif. During the past, the deformation of the Maya Plate, have been attributed to the Laramide Orogeny. The present study develops a model based on the interaction between the Maya, Caribbean and the oceanic Cocos-plates, assuming that during the Middle Miocene, the development of the left-lateral Motagua Fault between the Maya and Caribbean plates, displaced the Yucatan Platform in a southwestward direction. It is consider that the combine action of two opposite forces, was responsible for the development of most of the Sierra de Chiapas major anticlines and synclines. Finally the model suggests that these structures, were later affected by trans-compressive forces, originated as a secondary response, due to the development of several left-lateral transcurrent faults, associated with the Polochic Fault System.

  20. Plate tectonics drive tropical reef biodiversity dynamics

    NASA Astrophysics Data System (ADS)

    Leprieur, Fabien; Descombes, Patrice; Gaboriau, Théo; Cowman, Peter F.; Parravicini, Valeriano; Kulbicki, Michel; Melián, Carlos J.; de Santana, Charles N.; Heine, Christian; Mouillot, David; Bellwood, David R.; Pellissier, Loïc

    2016-05-01

    The Cretaceous breakup of Gondwana strongly modified the global distribution of shallow tropical seas reshaping the geographic configuration of marine basins. However, the links between tropical reef availability, plate tectonic processes and marine biodiversity distribution patterns are still unknown. Here, we show that a spatial diversification model constrained by absolute plate motions for the past 140 million years predicts the emergence and movement of diversity hotspots on tropical reefs. The spatial dynamics of tropical reefs explains marine fauna diversification in the Tethyan Ocean during the Cretaceous and early Cenozoic, and identifies an eastward movement of ancestral marine lineages towards the Indo-Australian Archipelago in the Miocene. A mechanistic model based only on habitat-driven diversification and dispersal yields realistic predictions of current biodiversity patterns for both corals and fishes. As in terrestrial systems, we demonstrate that plate tectonics played a major role in driving tropical marine shallow reef biodiversity dynamics.

  1. Plate tectonics drive tropical reef biodiversity dynamics.

    PubMed

    Leprieur, Fabien; Descombes, Patrice; Gaboriau, Théo; Cowman, Peter F; Parravicini, Valeriano; Kulbicki, Michel; Melián, Carlos J; de Santana, Charles N; Heine, Christian; Mouillot, David; Bellwood, David R; Pellissier, Loïc

    2016-05-06

    The Cretaceous breakup of Gondwana strongly modified the global distribution of shallow tropical seas reshaping the geographic configuration of marine basins. However, the links between tropical reef availability, plate tectonic processes and marine biodiversity distribution patterns are still unknown. Here, we show that a spatial diversification model constrained by absolute plate motions for the past 140 million years predicts the emergence and movement of diversity hotspots on tropical reefs. The spatial dynamics of tropical reefs explains marine fauna diversification in the Tethyan Ocean during the Cretaceous and early Cenozoic, and identifies an eastward movement of ancestral marine lineages towards the Indo-Australian Archipelago in the Miocene. A mechanistic model based only on habitat-driven diversification and dispersal yields realistic predictions of current biodiversity patterns for both corals and fishes. As in terrestrial systems, we demonstrate that plate tectonics played a major role in driving tropical marine shallow reef biodiversity dynamics.

  2. Plate tectonics drive tropical reef biodiversity dynamics

    PubMed Central

    Leprieur, Fabien; Descombes, Patrice; Gaboriau, Théo; Cowman, Peter F.; Parravicini, Valeriano; Kulbicki, Michel; Melián, Carlos J.; de Santana, Charles N.; Heine, Christian; Mouillot, David; Bellwood, David R.; Pellissier, Loïc

    2016-01-01

    The Cretaceous breakup of Gondwana strongly modified the global distribution of shallow tropical seas reshaping the geographic configuration of marine basins. However, the links between tropical reef availability, plate tectonic processes and marine biodiversity distribution patterns are still unknown. Here, we show that a spatial diversification model constrained by absolute plate motions for the past 140 million years predicts the emergence and movement of diversity hotspots on tropical reefs. The spatial dynamics of tropical reefs explains marine fauna diversification in the Tethyan Ocean during the Cretaceous and early Cenozoic, and identifies an eastward movement of ancestral marine lineages towards the Indo-Australian Archipelago in the Miocene. A mechanistic model based only on habitat-driven diversification and dispersal yields realistic predictions of current biodiversity patterns for both corals and fishes. As in terrestrial systems, we demonstrate that plate tectonics played a major role in driving tropical marine shallow reef biodiversity dynamics. PMID:27151103

  3. Effect of calcium ions on the evolution of biofouling by Bacillus subtilis in plate heat exchangers simulating the heat pump system used with treated sewage in the 2008 Olympic Village.

    PubMed

    Tian, Lei; Chen, Xiao Dong; Yang, Qian Peng; Chen, Jin Chun; Shi, Lin; Li, Qiong

    2012-06-01

    Heat pump systems using treated sewage water as the heat source were used in the Beijing Olympic Village for domestic heating and cooling. However, considerable biofouling occurred in the plate heat exchangers used in the heat pump system, greatly limiting the system efficiency. This study investigates the biofouling characteristics using a plate heat exchanger in parallel with a flow cell system to focus on the effect of calcium ions on the biofilm development. The interactions between the microorganisms and Ca(2+) enhances both the extent and the rate of biofilm development with increasing Ca(2+) concentration, leading to increased heat transfer and flow resistances. Three stages of biofouling development were identified in the presence of Ca(2+) from different biofouling mass growth rates with an initial stage, a rapid growth stage and an extended growth stage. Each growth stage had different biofouling morphologies influenced by the Ca(2+) concentration. The effects of Ca(2+) on the biofouling heat transfer and flow resistances had a synergistic effect related to both the biofouling mass and the morphology. The effect of Ca(2+) on the biofouling development was most prominent during the rapid growth stage.

  4. Shuttle plate braiding machine

    NASA Technical Reports Server (NTRS)

    Huey, Jr., Cecil O. (Inventor)

    1994-01-01

    A method and apparatus for moving yarn in a selected pattern to form a braided article. The apparatus includes a segmented grid of stationary support elements and a plurality of shuttles configured to carry yarn. The shuttles are supported for movement on the grid assembly and each shuttle includes a retractable plunger for engaging a reciprocating shuttle plate that moves below the grid assembly. Such engagement at selected times causes the shuttles to move about the grid assembly in a selected pattern to form a braided article of a particular geometry.

  5. Crack Detection in Armor Plates Using Ultrasonic Techniques (PREPRINT)

    DTIC Science & Technology

    2007-03-01

    A method of using piezoelectric lead zirconate titanate (PZT) transducers to characterize the vibrational modes of Vehicle Body Armor Support System...VBASS) plates and its preliminary results are presented. The amplitude of the vibrational modes of undamaged plates are compared to the vibrational

  6. Crack Detection in Armor Plates Using Ultrasonic Techniques

    DTIC Science & Technology

    2008-06-01

    A method of using piezoelectric lead zirconate titanate (PZT) transducers to characterize the vibrational modes of ceramic Vehicle Body Armor Support...System (VBASS) plates for the purpose of crack detection is presented. The amplitudes of the vibrational modes undamaged plates are compared to the

  7. Earth's Decelerating Tectonic Plates (Invited)

    NASA Astrophysics Data System (ADS)

    Forte, A. M.; Moucha, R.; Rowley, D. B.; Quere, S.; Mitrovica, J. X.; Simmons, N. A.; Grand, S. P.

    2009-12-01

    We employ a recently developed convection model constrained by seismic, geodynamic and mineral physics data (Simmons et al., GJI 2009) to explore the impact of time-dependent changes in mantle buoyancy forces on tectonic plate accelerations. This plate-coupled mantle convection model incorporates a viscosity structure that reconciles both glacial isostatic adjustment and global convection-related data sets (Mitrovica & Forte, EPSL 2004) and it successfully reproduces present-day plate velocities, global surface gravity and topography data. This convection model predicts the recent deceleration of several major plates in the Pacific and Indo-Atlantic hemispheres. Independent verification of these predictions is a fundamental test of the plausibility of the buoyancy forces and rheological structure in the convection model. To this end, we consider marine magnetic anomaly and space geodetic constraints on tectonic plate motions to determine a new global map of present-day rates of change of plate velocities. This map shows that several major plates, such as the Pacific, Africa and Nazca plates are presently decelerating and that they contribute to a globally-averaged slowdown in tectonic plate speeds. These joint geologic-geodetic inferences of plate decelerations are consistent with those predicted by our tomography-based convection model.

  8. Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology.

    PubMed

    Ganjali Dashti, M; Abdeshahian, P; Sudesh, K; Phua, K K

    2016-01-01

    The objective of this study was to develop an optimized assay for Salmonella Typhi biofilm that mimics the environment of the gallbladder as an experimental model for chronic typhoid fever. Multi-factorial assays are difficult to optimize using traditional one-factor-at-a-time optimization methods. Response surface methodology (RSM) was used to optimize six key variables involved in S. Typhi biofilm formation on cholesterol-coated polypropylene 96-well microtiter plates. The results showed that bile (1.22%), glucose (2%), cholesterol (0.05%) and potassium chloride (0.25%) were critical factors affecting the amount of biofilm produced, but agitation (275 rpm) and sodium chloride (0.5%) had antagonistic effects on each other. Under these optimum conditions the maximum OD reading for biofilm formation was 3.4 (λ600 nm), and the coefficients of variation for intra-plate and inter-plate assays were 3% (n = 20) and 5% (n = 8), respectively. These results showed that RSM is an effective approach for biofilm assay optimization.

  9. Adaptive Focused Acoustics (AFA) Improves the Performance of Microtiter Plate ELISAs.

    PubMed

    Green, David J; Rudd, Edwin A; Laugharn, James A

    2014-08-01

    We investigated the use of Adaptive Focused Acoustics (AFA) technology to improve the performance of microtiter plate enzyme-linked immunosorbent assays (ELISAs). Experiments were performed with commercially available AFA instrumentation and off-the-shelf 96-well microtiter plate sandwich ELISAs. AFA was applied over a range of acoustic energies, temperatures, and durations to the antigen/antibody binding step of an ELISA for measuring HIV-1 p24 in tissue culture samples. AFA-mediated antigen/antibody binding was enhanced up to 2-fold over passive binding at comparable temperatures and was superior or comparable at low temperature (8-10 °C) to passive binding at 37 °C. Lower nonspecific binding (NSB), lower inter- and intra-assay coefficients of variation (CVs), higher Z' factors, and lower limits of detection (LODs) were measured in AFA-mediated assays compared with conventional passive binding. In a more limited study, AFA enhancement of antigen/antibody binding and lower NSB was measured in an ELISA for measuring IGFBP-3 in human plasma. We conclude from this study that application of AFA to antigen/antibody binding steps in microtiter plate ELISAs can enhance key assay performance parameters, particularly Z' factors and LODs. These features render AFA-mediated binding assays potentially more useful in applications such as high-throughput screening and in vitro diagnostics than assays processed with conventional passive antigen/antibody binding steps.

  10. Plating on difficult-to-plate metals: what's new

    SciTech Connect

    Wiesner, H.J.

    1980-07-30

    Some of the changes since 1970 in procedures for plating on such materials as titanium, molybdenum, silicon, aluminum, and gallium arsenide are summarized. While basic procedures for plating some of these materials were developed as many as 30 to 40 years ago, changes in the end uses of the plated products have necessitated new plating processes. In some cases, vacuum techniques - such as ion bombardment, ion implantation, and vacuum metallization - have been introduced to improve the adhesion of electrodeposits. In other cases, these techniques have been used to deposit materials upon which electrodeposits are required.

  11. Yield surfaces for anisotropic plates

    NASA Astrophysics Data System (ADS)

    Walker, J. D.; Thacker, B. H.

    2000-04-01

    Aerospace systems are incorporating composite materials into their structures. The composite materials are often anisotropic in mechanical response due to their geometric layout. For many years, the failure surfaces of anisotropic materials were thought to be characterizable by a quadratic function in the stress, referred to as a Tsai-Wu yield surface, or, in a more restrictive form, a Tsai-Hill yield surface. Such a representation does not work for materials that are strong in two directions and weak in one direction, which is the case of most interest since it represents fiber/epoxy composite plates. This paper demonstrates the impossibility of modeling the failure surface with either the Tsai-Wu or Tsai-Hill failure surfaces. A yield surface is presented based on the lemniscate, which is quartic in the stress. This new yield surface addresses the case of strong in two directions and weak in one.

  12. LOWLID FORMATION AND PLATE TECTONICS ON EXOPLANETS

    NASA Astrophysics Data System (ADS)

    Stamenkovic, V.; Noack, L.; Breuer, D.

    2009-12-01

    The last years of astronomical observation have opened the doors to a universe filled with extrasolar planets. Detection techniques still only offer the possibility to detect mainly Super-Earths above five Earth masses. But detection techniques do steadily improve and are offering the possibility to detect even smaller planets. The observations show that planets seem to exist in many possible sizes just as the planets and moons of our own solar system do. It is only a natural question to ask if planetary mass has an influence on some key habitability factors such as on plate tectonics, allowing us to test which exoplanets might be more likely habitable than others, and allowing us to understand if plate tectonics on Earth is a stable or a critical, instable process that could easily be perturbed. Here we present results derived from 1D parameterized thermal evolution and 2D/3D computer models, showing how planetary mass influences the propensity of plate tectonics for planets with masses ranging from 0.1 to 10 Earth masses. Lately [2, 3] studied the effect of planetary mass on the ability to break plates and hence initiate plate tectonics - but both derived results contradictory to the other. We think that one of the reasons why both studies [2, 3] are not acceptable in their current form is partly due to an oversimplification. Both treated viscosity only temperature-dependent but neglected the effect pressure has on enlarging the viscosity in the deep mantle. More massive planets have therefore a stronger pressure-viscosity-coupling making convection at high pressures sluggish or even impossible. For planets larger than two Earth masses we observe that a conductive lid (termed low-lid) forms above the core-mantle boundary and thus reduces the effective convective part of the mantle when including a pressure-dependent term into the viscosity laws as shown in [1]. Moreover [2, 3] use time independent steady state models neglecting the fact that plate tectonics is a

  13. Localised Plate Motion on Venus

    NASA Astrophysics Data System (ADS)

    Ghail, R. C.

    1996-03-01

    The volcanic and tectonic features observed in Dali Vinculum, Parga Vinculum and Imdr Regio are concentrated at long, narrow, curvilinear zones, with relatively minor volcanism and tectonism between these zones. These zones, whilst more diffuse than terrestrial plate boundaries, nevertheless define the margins of tectonic plates. In contrast to Earth, however, it appears that venusian plates are neither created nor destroyed by lateral motion. Rather, plates are thinned and intruded at vincula plate boundaries, vertically accreted by small-scale intra-plate (planitia) volcanism and perhaps destroyed by delamination of thickened crust in tesserae and montane regions such as Thetis Regio and Ishtar Terra. The diversity in age both between and within these three areas together with the evidence for infrequent, small scale resurfacing in the planitiae are difficult to reconcile with a non-uniformitarian geological process.

  14. Tectonics of the Easter plate

    NASA Technical Reports Server (NTRS)

    Engeln, J. F.; Stein, S.

    1984-01-01

    A new model for the Easter plate is presented in which rift propagation has resulted in the formation of a rigid plate between the propagating and dying ridges. The distribution of earthquakes, eleven new focal mechanisms, and existing bathymetric and magnetic data are used to describe the tectonics of this area. Both the Easter-Nazca and Easter-Pacific Euler poles are sufficiently close to the Easter plate to cause rapid changes in rates and directions of motion along the boundaries. The east and west boundaries are propagating and dying ridges; the southwest boundary is a slow-spreading ridge and the northern boundary is a complex zone of convergent and transform motion. The Easter plate may reflect the tectonics of rift propagation on a large scale, where rigid plate tectonics requires boundary reorientation. Simple schematic models to illustrate the general features and processes which occur at plates resulting from large-scale rift propagation are used.

  15. On electrostatically actuated NEMS/MEMS circular plates

    NASA Astrophysics Data System (ADS)

    Caruntu, Dumitru I.; Alvarado, Iris

    2011-04-01

    This paper deals with electrostatically actuated micro and nano-electromechanical (MEMS/NEMS) circular plates. The system under investigation consists of two bodies, a deformable and conductive circular plate placed above a fixed, rigid and conductive ground plate. The deformable circular plate is electrostatically actuated by applying an AC voltage between the two plates. Nonlinear parametric resonance and pull-in occur at certain frequencies and relatively large AC voltage, respectively. Such phenomena are useful for applications such as sensors, actuators, switches, micro-pumps, micro-tweezers, chemical and mass sensing, and micro-mirrors. A mathematical model of clamped circular MEMS/NEMS electrostatically actuated plates has been developed. Since the model is in the micro- and nano-scale, surface forces, van der Waals and/or Casimir, acting on the plate are included. A perturbation method, the Method of Multiple Scales (MMS), is used for investigating the case of weakly nonlinear MEMS/NEMS circular plates. Two time scales, fast and slow, are considered in this work. The amplitude-frequency and phase-frequency response of the plate in the case of primary resonance are obtained and discussed.

  16. Solution of non-rectangular plates with macroelement method

    NASA Astrophysics Data System (ADS)

    Delyavskyy, Mykhaylo; Rosinski, Krystian

    2017-03-01

    New approach to static analysis of thin non-rectangular arbitrarily loaded plates, called the macroelement method, has been developed in this paper. Macroelement is a rectangular plate which entirely contains real plate. The mathematical model of macroelement was built. The equilibrium equations are performed for macroelement and boundary conditions are written on the line corresponding to contour of real plate in the nodes which are zero points of trigonometric functions, included in the macroelement model. The load is applied only to separate nodes on the surface of real plate, whereas the complement of a plate to macroelement is unloaded. Analysis of construction is reduced to solving a system of linear algebraic equations. The method provides better accuracy compared to finite element method and requires less equations. There is trapeze plate clamped at inclined edge and simply supported at opposite one considered in this paper. The other edges of the plate are free. Uniformly distributed load on the surface of real plate is taken into account.

  17. Channel plate for DNA sequencing

    DOEpatents

    Douthart, Richard J.; Crowell, Shannon L.

    1998-01-01

    This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface.

  18. Dissolution of HFIR control plates

    SciTech Connect

    Posey, J.C.

    1984-03-01

    A process was developed for the dissolution of High Flux Isotope Reactor (HFIR) control plates. These plates consist of aluminum metal, intensely radioactive europium oxide, and a small amount of tantalum metal. The radioactive solution will be diluted, mixed with grout, and disposed of by shale fracture. The plates are dissolved in nitric acid using a mercury catalyst. Conditions were determined that would produce a reaction rate compatible with existing equipment. 3 references, 1 figure, 3 tables.

  19. Channel plate for DNA sequencing

    DOEpatents

    Douthart, R.J.; Crowell, S.L.

    1998-01-13

    This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface. 15 figs.

  20. Ion plating for the future

    NASA Technical Reports Server (NTRS)

    Spalvins, T.

    1981-01-01

    The ion plating techniques are classified relative to the instrumental set up, evaporation media, and mode of transport. A distinction is drawn between the low vacuum (plasma) and high vacuum (ion beam) techniques. Ion plating technology is discussed at the fundamental and industrial level. At the fundamental level, the capabilities and limitations of the plasma (evaporant flux) and film characteristics are evaluated. And on the industrial level, the performance and potential uses of ion plated films are discussed.

  1. Plate tectonics and planetary habitability: current status and future challenges.

    PubMed

    Korenaga, Jun

    2012-07-01

    Plate tectonics is one of the major factors affecting the potential habitability of a terrestrial planet. The physics of plate tectonics is, however, still far from being complete, leading to considerable uncertainty when discussing planetary habitability. Here, I summarize recent developments on the evolution of plate tectonics on Earth, which suggest a radically new view on Earth dynamics: convection in the mantle has been speeding up despite its secular cooling, and the operation of plate tectonics has been facilitated throughout Earth's history by the gradual subduction of water into an initially dry mantle. The role of plate tectonics in planetary habitability through its influence on atmospheric evolution is still difficult to quantify, and, to this end, it will be vital to better understand a coupled core-mantle-atmosphere system in the context of solar system evolution.

  2. [Macroscopic and functional anatomy of the apophyseal plate].

    PubMed

    Putz, R; Milz, S

    2016-03-01

    Apophyses are growth zones attached to the shaft (corpus) of larger bones. They vary in size and develop their own ossification centres or form as part of an usually fibrocartilaginous tendon or ligament insertion. The structure of the cartilaginous apophyseal plate is very similar to that of an epiphyseal growth plate and like these they are adapted to withstand perpendicular compressive forces without becoming harmed. This is best highlighted by the fact that their mineralized borders always orient themselves perpendicular to the overall resulting force vector. The edges of the apophyseal plates are characteristically bent which allows them to resist moderate shear forces. Like the epiphyseal plates the apophyseal plates exhibit a zonal organization which is not very well adapted to permanently withstand shear forces, especially if they occur under dynamic conditions. In these situations the tendinous insertions with their collagen fibre anchoring system have to provide compensation when balancing the load transmitted across the system.

  3. Laser-driven flyer plate

    DOEpatents

    Paisley, Dennis L.

    1991-01-01

    Apparatus for producing high velocity flyer plates involving placing a layer of dielectric material between a first metal foil and a second metal foil. With laser irradiation through an optical substrate, the first metal foil forms a plasma in the area of the irradiation, between the substrate and the solid portion of the first metal foil. When the pressure between the substrate and the foil reaches the stress limit of the dielectric, the dielectric will break away and launch the flyer plate out of the second metal foil. The mass of the flyer plate is controlled, as no portion of the flyer plate is transformed into a plasma.

  4. True Shear Parallel Plate Viscometer

    NASA Technical Reports Server (NTRS)

    Ethridge, Edwin; Kaukler, William

    2010-01-01

    This viscometer (which can also be used as a rheometer) is designed for use with liquids over a large temperature range. The device consists of horizontally disposed, similarly sized, parallel plates with a precisely known gap. The lower plate is driven laterally with a motor to apply shear to the liquid in the gap. The upper plate is freely suspended from a double-arm pendulum with a sufficiently long radius to reduce height variations during the swing to negligible levels. A sensitive load cell measures the shear force applied by the liquid to the upper plate. Viscosity is measured by taking the ratio of shear stress to shear rate.

  5. Glass-bead peen plating

    NASA Technical Reports Server (NTRS)

    Graves, J. R.

    1974-01-01

    Peen plating of aluminum, copper, and nickel powders was investigated. Only aluminum was plated successfully within the range of peen plating conditions studied. Optimum plating conditions for aluminum were found to be: (1) bead/powder mixture containing 25 to 35% powder by weight, (2) peening intensity of 0.007A as measured by Almen strip, and (3) glass impact bead diameter of at least 297 microns (0.0117 inches) for depositing-100 mesh aluminum powder. No extensive cleaning or substrate preparation is required beyond removing loose dirt or heavy oil.

  6. Carbon-assisted flyer plates

    DOEpatents

    Stahl, David B.; Paisley, Dennis L.

    1994-01-01

    A laser driven flyer plate utilizing an optical fiber connected to a laser. The end of the optical fiber has a layer of carbon and a metal layer deposited onto it. The carbon layer provides the laser induced plasma which is superior to the plasma produced from most metals. The carbon layer plasma is capable of providing a flatter flyer plate, converting more of the laser energy to driving plasma, promoting a higher flyer plate acceleration, and providing a more uniform pulse behind the plate. In another embodiment, the laser is in optical communication with a substrate onto which a layer of carbon and a layer of metal have been deposited.

  7. BEPLATE emdash simulation of electrochemical plating

    SciTech Connect

    Giles, G.E. ); Gray, L.J. ); Bullock, J.S. IV )

    1990-09-01

    BEPLATE is a FORTRAN code that uses the boundary element method to simulate the electrochemical plating of material on parts, primarily rotating axisymmetric parts. A boundary element technique is used to solve for the local current density and thus the plating rate on the part, which is used to calculate the growth in the plated layer over a user-specified time step. The surface is moved to reflect this growth, and the new surface is used to generate the local current density. This cycle is repeated until the final time specified by the analyst, producing the final plated thickness. BEPLATE includes models for the polarization effects at both the part (cathode) and anode and allows the use of symmetry planes and nonconducting shields. For electroplating simulations, the part shape is normally assumed to be axisymmetric with a centerline along the z-axis. More general part shapes can be analyzed by BEPLATE if the surface growth simulation is not needed. In either case, the shield, anode, and tank geometries are not restricted to specific shapes. This report includes the information required to run BEPLATE, specifically, a brief description of the BEPLATE system including hardware and software requirements, a description of the complete simulation process, discussion of rules for generating models, and additional reference material. This system of codes consists of model generators (PIGS or PATRAN), input processor (BEPIN), the simulation code (BEPLATE) and postprocessing codes (PATRAN or CONPLOT).

  8. Wave interaction with dual circular porous plates

    NASA Astrophysics Data System (ADS)

    Mondal, Arpita; Gayen, R.

    2015-12-01

    In this paper we investigated the reflection and the transmission of a system of two symmetric circular-arc-shaped thin porous plates submerged in deep water within the context of linear theory. The hypersingular integral equation technique has been used to analyze the problem mathematically. The integral equations are formulated by applying Green's integral theorem to the fundamental potential function and the scattered potential function into a suitable fluid region, and then using the boundary condition on the porous plate surface. These are solved approximately using an expansion-cum-collocation method using the behaviour of the potential functions at the tips of the plates. This method ultimately produces a very good numerical approximation for the reflection and the transmission coefficients and hydrodynamic force components. The numerical results are depicted graphically against the wave number for a variety of layouts of the arc. Some results are compared with known results for similar configurations of dual rigid plate systems available in the literature with good agreement.

  9. Scintillator plate calorimetry

    SciTech Connect

    Price, L.E.

    1990-01-01

    Calorimetry using scintillator plates or tiles alternated with sheets of (usually heavy) passive absorber has been proven over multiple generations of collider detectors. Recent detectors including UA1, CDF, and ZEUS have shown good results from such calorimeters. The advantages offered by scintillator calorimetry for the SSC environment, in particular, are speed (<10 nsec), excellent energy resolution, low noise, and ease of achieving compensation and hence linearity. On the negative side of the ledger can be placed the historical sensitivity of plastic scintillators to radiation damage, the possibility of nonuniform response because of light attenuation, and the presence of cracks for light collection via wavelength shifting plastic (traditionally in sheet form). This approach to calorimetry is being investigated for SSC use by a collaboration of Ames Laboratory/Iowa State University, Argonne National Laboratory, Bicron Corporation, Florida State University, Louisiana State University, University of Mississippi, Oak Ridge National Laboratory, Virginia Polytechnic Institute and State University, Westinghouse Electric Corporation, and University of Wisconsin.

  10. Intermittent plate tectonics?

    PubMed

    Silver, Paul G; Behn, Mark D

    2008-01-04

    Although it is commonly assumed that subduction has operated continuously on Earth without interruption, subduction zones are routinely terminated by ocean closure and supercontinent assembly. Under certain circumstances, this could lead to a dramatic loss of subduction, globally. Closure of a Pacific-type basin, for example, would eliminate most subduction, unless this loss were compensated for by comparable subduction initiation elsewhere. Given the evidence for Pacific-type closure in Earth's past, the absence of a direct mechanism for termination/initiation compensation, and recent data supporting a minimum in subduction flux in the Mesoproterozoic, we hypothesize that dramatic reductions or temporary cessations of subduction have occurred in Earth's history. Such deviations in the continuity of plate tectonics have important consequences for Earth's thermal and continental evolution.

  11. From Neural Plate to Cortical Arousal—A Neuronal Network Theory of Sleep Derived from in Vitro “Model” Systems for Primordial Patterns of Spontaneous Bioelectric Activity in the Vertebrate Central Nervous System

    PubMed Central

    Corner, Michael A.

    2013-01-01

    In the early 1960s intrinsically generated widespread neuronal discharges were discovered to be the basis for the earliest motor behavior throughout the animal kingdom. The pattern generating system is in fact programmed into the developing nervous system, in a regionally specific manner, already at the early neural plate stage. Such rhythmically modulated phasic bursts were next discovered to be a general feature of developing neural networks and, largely on the basis of experimental interventions in cultured neural tissues, to contribute significantly to their morpho-physiological maturation. In particular, the level of spontaneous synchronized bursting is homeostatically regulated, and has the effect of constraining the development of excessive network excitability. After birth or hatching, this “slow-wave” activity pattern becomes sporadically suppressed in favor of sensory oriented “waking” behaviors better adapted to dealing with environmental contingencies. It nevertheless reappears periodically as “sleep” at several species-specific points in the diurnal/nocturnal cycle. Although this “default” behavior pattern evolves with development, its essential features are preserved throughout the life cycle, and are based upon a few simple mechanisms which can be both experimentally demonstrated and simulated by computer modeling. In contrast, a late onto- and phylogenetic aspect of sleep, viz., the intermittent “paradoxical” activation of the forebrain so as to mimic waking activity, is much less well understood as regards its contribution to brain development. Some recent findings dealing with this question by means of cholinergically induced “aroused” firing patterns in developing neocortical cell cultures, followed by quantitative electrophysiological assays of immediate and longterm sequelae, will be discussed in connection with their putative implications for sleep ontogeny. PMID:24961426

  12. From neural plate to cortical arousal-a neuronal network theory of sleep derived from in vitro "model" systems for primordial patterns of spontaneous bioelectric activity in the vertebrate central nervous system.

    PubMed

    Corner, Michael A

    2013-05-22

    In the early 1960s intrinsically generated widespread neuronal discharges were discovered to be the basis for the earliest motor behavior throughout the animal kingdom. The pattern generating system is in fact programmed into the developing nervous system, in a regionally specific manner, already at the early neural plate stage. Such rhythmically modulated phasic bursts were next discovered to be a general feature of developing neural networks and, largely on the basis of experimental interventions in cultured neural tissues, to contribute significantly to their morpho-physiological maturation. In particular, the level of spontaneous synchronized bursting is homeostatically regulated, and has the effect of constraining the development of excessive network excitability. After birth or hatching, this "slow-wave" activity pattern becomes sporadically suppressed in favor of sensory oriented "waking" behaviors better adapted to dealing with environmental contingencies. It nevertheless reappears periodically as "sleep" at several species-specific points in the diurnal/nocturnal cycle. Although this "default" behavior pattern evolves with development, its essential features are preserved throughout the life cycle, and are based upon a few simple mechanisms which can be both experimentally demonstrated and simulated by computer modeling. In contrast, a late onto- and phylogenetic aspect of sleep, viz., the intermittent "paradoxical" activation of the forebrain so as to mimic waking activity, is much less well understood as regards its contribution to brain development. Some recent findings dealing with this question by means of cholinergically induced "aroused" firing patterns in developing neocortical cell cultures, followed by quantitative electrophysiological assays of immediate and longterm sequelae, will be discussed in connection with their putative implications for sleep ontogeny.

  13. 10. DETAIL OF BUILDER'S PLATE AT NORTH PORTAL. PLATE READS: ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. DETAIL OF BUILDER'S PLATE AT NORTH PORTAL. PLATE READS: 1889, BUILT BY THE BERLIN IRON BRIDGE CO. EAST BERLIN CONN. DOUGLAS & JARVIS PAT. APT. 16, 1878, AP'L 17, 1885. A.P. FORESMAN, WM. S. STARR, T.J. STREBEIGH, COMMISSIONERS. - Pine Creek Bridge, River Road spanning Pine Creek, Jersey Shore, Lycoming County, PA

  14. Maskless, parallel patterning with zone-plate array lithography

    SciTech Connect

    Carter, D. J. D.; Gil, Dario; Menon, Rajesh; Mondol, Mark K.; Smith, Henry I.; Anderson, Erik H.

    1999-11-01

    Zone-plate array lithography (ZPAL) is a maskless lithography scheme that uses an array of shuttered zone plates to print arbitrary patterns on a substrate. An experimental ultraviolet ZPAL system has been constructed and used to simultaneously expose nine different patterns with a 3x3 array of zone plates in a quasidot-matrix fashion. We present exposed patterns, describe the system design and construction, and discuss issues essential to a functional ZPAL system. We also discuss another ZPAL system which operates with 4.5 nm x radiation from a point source. We present simulations which show that, with our existing x-ray zone plates and this system, we should be able to achieve 55 nm resolution. (c) 1999 American Vacuum Society.

  15. Are allogenic or xenogenic screws and plates a reasonable alternative to alloplastic material for osteosynthesis--a histomorphological analysis in a dynamic system.

    PubMed

    Jacobsen, C; Obwegeser, J A

    2010-12-01

    Despite invention of titanium and resorbable screws and plates, still, one of the main challenges in bone fixation is the search for an ideal osteosynthetic material. Biomechanical properties, biocompatibility, and also cost effectiveness and clinical practicability are factors for the selection of a particular material. A promising alternative seems to be screws and plates made of bone. Recently, xenogenic bone pins and screws have been invented for use in joint surgery. In this study, screws made of allogenic sheep and xenogenic human bone were analyzed in a vital and dynamic sheep-model and compared to conventional titanium screws over a standard period of bone healing of 56 days with a constant applied extrusion force. Biomechanical analysis and histomorphological evaluation were performed. After 56 days of insertion xenogenic screws made of human bone showed significantly larger distance of extrusion of on average 173.8 μm compared to allogenic screws made of sheep bone of on average 27.8 and 29.95 μm of the titanium control group. Severe resorption processes with connective tissue interposition were found in the histomorphological analysis of the xenogenic screws in contrast to new bone formation and centripetal vascularization of the allogenic bone screw, as well as in processes of incorporation of the titanium control group. The study showed allogenic cortical bone screws as a substantial alternative to titanium screws with good biomechanical properties. In contrast to other reports a different result was shown for the xenogenic bone screws. They showed insufficient holding strength with confirmative histomorphological signs of degradation and insufficient osseointegration. Before common clinical use of xenogenic osteosynthetic material, further evaluation should be performed.

  16. Plate tectonics, damage and inheritance.

    PubMed

    Bercovici, David; Ricard, Yanick

    2014-04-24

    The initiation of plate tectonics on Earth is a critical event in our planet's history. The time lag between the first proto-subduction (about 4 billion years ago) and global tectonics (approximately 3 billion years ago) suggests that plates and plate boundaries became widespread over a period of 1 billion years. The reason for this time lag is unknown but fundamental to understanding the origin of plate tectonics. Here we suggest that when sufficient lithospheric damage (which promotes shear localization and long-lived weak zones) combines with transient mantle flow and migrating proto-subduction, it leads to the accumulation of weak plate boundaries and eventually to fully formed tectonic plates driven by subduction alone. We simulate this process using a grain evolution and damage mechanism with a composite rheology (which is compatible with field and laboratory observations of polycrystalline rocks), coupled to an idealized model of pressure-driven lithospheric flow in which a low-pressure zone is equivalent to the suction of convective downwellings. In the simplest case, for Earth-like conditions, a few successive rotations of the driving pressure field yield relic damaged weak zones that are inherited by the lithospheric flow to form a nearly perfect plate, with passive spreading and strike-slip margins that persist and localize further, even though flow is driven only by subduction. But for hotter surface conditions, such as those on Venus, accumulation and inheritance of damage is negligible; hence only subduction zones survive and plate tectonics does not spread, which corresponds to observations. After plates have developed, continued changes in driving forces, combined with inherited damage and weak zones, promote increased tectonic complexity, such as oblique subduction, strike-slip boundaries that are subparallel to plate motion, and spalling of minor plates.

  17. Saliva-based system for health and toxicology monitoring

    NASA Astrophysics Data System (ADS)

    Fenner, D. B.; Stevens, A. E.; Rosen, D. I.; Ferrante, A. A.; Davis, S. J.

    2009-05-01

    The practical utility of technologies for early detection of human exposure to a variety of toxic agents has been limited in many cases by the absence of instruments suitable for first responders and at field hospitals. Microarrays provide multiplexed assay of a large number of human biomarkers, including cytokines and chemokines, indicators of immune system health. Assay of saliva is less invasive and provides quick indication of exposure especially of the respiratory system. Our pilot clinical study has uncovered an early cytokine response in human saliva. As a model for respiratory exposure, a cohort of 16 adult volunteers was challenged with FluMistTM vaccinations, an FDA approved, attenuated live influenza virus. Blood and saliva cytokine levels were monitored immediately prior to and up to 7 days afterwards. Bead assay found little change in blood cytokine levels while several of those in saliva were frequently elevated above two standard deviations on trial days one and three. We have developed a prototype portable saliva monitoring system consisting of microarray cytokine capture plate, luminescent reporter, and whole plate imaging. Assay is with a commercial 96-well plate spotted with up to 16 distinct biomarkers per well and read by chemiluminescence. A battery-powered, 16-bit, cooled-CCD camera and laptop PC provide imaging and data reduction. Detection limits of common inflammatory cytokines were measured at about 1-5 pg/ml which is within the clinically significant range for saliva of exposed individuals, as verified for samples from the small clinical trial. An expanded study of cytokine response in saliva of therapeutic radiation oncology patients is being launched.

  18. Metals plated on fluorocarbon polymers

    NASA Technical Reports Server (NTRS)

    Ford, H.; Krasinsky, J. B.; Vango, S. P.

    1964-01-01

    Electroplating lead on fluorocarbon polymer parts is accomplished by etching the parts to be plated with sodium, followed by successive depositions of silver and lead from ultrasonically agitated plating solutions. Metals other than lead may be electroplated on the silvered parts.

  19. Gold plating on spectacle frames.

    PubMed

    Kenny, I; Mitchell, J W; Walsh, G

    1997-05-01

    An investigation was carried out into the thickness and standard of application of the plating and lacquer coatings applied to three metal spectacle frames. All conform to BS 6625 (1991) for plating thickness, but there was considerable variation in regularity and porosity.

  20. An Optimized High Throughput Clean-Up Method Using Mixed-Mode SPE Plate for the Analysis of Free Arachidonic Acid in Plasma by LC-MS/MS.

    PubMed

    Wang, Wan; Qin, Suzi; Li, Linsen; Chen, Xiaohua; Wang, Qunjie; Wei, Junfu

    2015-01-01

    A high throughput sample preparation method was developed utilizing mixed-mode solid phase extraction (SPE) in 96-well plate format for the determination of free arachidonic acid in plasma by LC-MS/MS. Plasma was mixed with 3% aqueous ammonia and loaded into each well of 96-well plate. After washing with water and methanol sequentially, 3% of formic acid in acetonitrile was used to elute arachidonic acid. The collected fraction was injected onto a reversed phase column at 30°C with mobile phase of acetonitrile/water (70 : 30, v/v) and detected by LC-MS/MS coupled with electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The calibration curve ranged from 10 to 2500 ng/mL with sufficient linearity (r (2) = 0.9999). The recoveries were in the range of 99.38% to 103.21% with RSD less than 6%. The limit of detection is 3 ng/mL.