76 FR 59008 - Airworthiness Directives; Airbus Model A300 B4-600, B4-600R, and F4-600R Series Airplanes, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-23

...-bonding between the skin and honeycomb core. Such reworks were also performed on some rudders fitted on... as a result of de-bonding between the skin and honeycomb core. Such reworks were also performed on..., depending on findings, the application of corrective actions for those rudders where production reworks have...

75 FR 49370 - Airworthiness Directives; Airbus Model A300 B4-600, B4-600R, and F4-600R Series Airplanes, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-13

.... American Airlines states that this will allow for more efficient planning and will relieve the burden of obtaining special flight permits to move re-activated airplanes to facilities where the inspections are to.... We have added a compliance time of within 840 flight hours after the effective date of this AD to...

76 FR 42029 - Airworthiness Directives; Airbus Model A300 B4-600, B4-600R, and F4-600R Series Airplanes, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-18

... load path, which would likely result in loss of control of the aeroplane. For the reasons explained... failure of the secondary load path, which would likely result in loss of control of the aeroplane... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2011...

76 FR 18960 - Airworthiness Directives; Airbus Model A300 B4-600, B4-600R, and F4-600R Series Airplanes, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-06

... unsafe condition is damage to wiring in the wing, center, and trim fuel tanks, due to failed P-clips used..., center, or trim fuel tanks. The proposed AD would require actions that are intended to address the unsafe..., 2009]. The unsafe condition is damage to wiring in the wing, center, and trim fuel tanks, due to failed...

76 FR 39248 - Airworthiness Directives; Airbus Model A300 B4-600, B4-600R, and F4-600R Series Airplanes, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-06

... after receipt. List of Subjects in 14 CFR Part 39 Air transportation, Aircraft, Aviation safety... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010... AGENCY: Federal Aviation Administration (FAA), Department of Transportation (DOT). ACTION: Final rule...

75 FR 32266 - Airworthiness Directives; Airbus Model A300 Series Airplanes; Airbus Model A300 B4-600, B4-600R...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-08

... after receipt. List of Subjects in 14 CFR Part 39 Air transportation, Aircraft, Aviation safety... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010... Transportation (DOT). ACTION: Final rule. SUMMARY: We are adopting a new airworthiness directive (AD) for the...

75 FR 61975 - Airworthiness Directives; Airbus Model A300 B4-600 Series Airplanes, Model A300 B4-600R Series...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-07

... contains regulatory documents #0;having general applicability and legal effect, most of which are keyed #0... programme (42,500 FC [flight cycles]), it has been concluded that a reinforcement of the junction of frame... Goal (ESG). * * * [Failure of the frame base], if not corrected, could affect the structural integrity...

75 FR 38061 - Airworthiness Directives; Airbus Model A300 B4-600 Series Airplanes; Model A300 B4-600R Series...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-01

... dimensional measurement of the holes, and doing corrective actions if necessary; doing an eddy current... dimensional measurement of the holes, doing an eddy current inspection of the holes for cracking, doing a cold... the effective date of this AD, prior to doing any cold working process, determine if an eddy current...

76 FR 6549 - Airworthiness Directives; Airbus Model A300 B4-600 and A300 B4-600R Series Airplanes, Model A300...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-07

... similar to the Airworthiness Limitations Items (ALI) Tasks 53.16.04-01-1 and 53.16.22- 01-1. We do not... determined that accomplishing the actions required by this AD terminates ALI Tasks 53.16.04-01-1 and 53.16.22... of this AD terminates ALI Tasks 53.16.04-01-1 and 53.16.22-01-1. We have reidentified subsequent...

75 FR 51705 - Airworthiness Directives; Airbus Model A300 B4-600 and A300 B4-600R Series Airplanes, Model A300...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-23

... FR (frame) 58 is necessary to enable the aeroplane to reach the Extended Service Goal (ESG). The... may send comments by any of the following methods: Federal eRulemaking Portal: Go to http://www... reinforcement of the upper fuselage circumferential joint at FR (frame) 58 is necessary to enable the aeroplane...

75 FR 51698 - Airworthiness Directives; Airbus Model A310 Series Airplanes; and Model A300 B4-600, A300 B4-600R...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-23

... the secondary load path, which would likely result in loss of control of the aeroplane. The proposed... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010... demonstration of the THSA upper attachment primary load path elements, only a gimbal having a thickness of 70mm...

76 FR 22302 - Airworthiness Directives; Airbus Model A310 Series Airplanes; and Model A300 B4-600, A300 B4-600R...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-21

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010... of 70mm (2.76 in). During the fatigue life demonstration of the THSA upper attachment primary load path elements, only a gimbal having a thickness of 70mm (2.76 in) was used. Thereafter, no additional...

34 CFR 600.4 - Institution of higher education.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 3 2011-07-01 2011-07-01 false Institution of higher education. 600.4 Section 600.4 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION INSTITUTIONAL ELIGIBILITY UNDER THE HIGHER EDUCATION ACT OF 1965, AS...

34 CFR 600.4 - Institution of higher education.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 34 Education 3 2012-07-01 2012-07-01 false Institution of higher education. 600.4 Section 600.4 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION INSTITUTIONAL ELIGIBILITY UNDER THE HIGHER EDUCATION ACT OF 1965, AS...

34 CFR 600.4 - Institution of higher education.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 34 Education 3 2013-07-01 2013-07-01 false Institution of higher education. 600.4 Section 600.4 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION INSTITUTIONAL ELIGIBILITY UNDER THE HIGHER EDUCATION ACT OF 1965, AS...

34 CFR 600.4 - Institution of higher education.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 34 Education 3 2014-07-01 2014-07-01 false Institution of higher education. 600.4 Section 600.4 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION INSTITUTIONAL ELIGIBILITY UNDER THE HIGHER EDUCATION ACT OF 1965, AS...

34 CFR 600.4 - Institution of higher education.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false Institution of higher education. 600.4 Section 600.4 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION INSTITUTIONAL ELIGIBILITY UNDER THE HIGHER EDUCATION ACT OF 1965, AS...

Synthesis and characterization of nanocrystalline Al 2024-B4C composite powders by mechanical alloying

NASA Astrophysics Data System (ADS)

Varol, T.; Canakci, A.

2013-06-01

In the present work, the effect of milling parameters on the morphology and microstructure of nanostructure Al2024-B4C composite powders obtained by mechanical alloying (MA) was studied. The effects of milling time and B4C content on the morphology, microstructure and particle size of nanostructure Al2024-B4C composite powders have been investigated. Different amounts of B4C particles (0, 5, 10 and 20 wt.%) were mixed with Al2024 powders and milled in a planetary ball mill for 30, 60, 120, 300, 420 and 600 min. Al 2024-B4C composite powders were characterized using a scanning electron microscope (SEM), laser particle-size analyzer, X-ray diffraction analysis (XRD) and the Vickers microhardness test. The results showed that the nanostructure Al2024-B4C composite powders were produced when they were milled for 600 min. The size of composite powder in the milled powder mixture was affected by the milling time and content of B4C particles. Moreover, it was observed that when MA reached a steady state, the properties of composite powders were stabilized.

77 FR 24137 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-23

... Directives; Airbus Airplanes AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule... Register. That AD applies to all Airbus Model A300 B4-600, B4-600R, and F4-600R series airplanes, Model... Airbus Model A300 B4-600, B4- 600R, and F4-600R series airplanes, Model A300 C4-605R Variant F airplanes...

50 CFR 600.325 - National Standard 4-Allocations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... communities, efficiency of various types of gear used in the fishery, transferability of effort to and impact... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false National Standard 4-Allocations. 600.325 Section 600.325 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND...

50 CFR 600.325 - National Standard 4-Allocations.

Code of Federal Regulations, 2011 CFR

2011-10-01

... communities, efficiency of various types of gear used in the fishery, transferability of effort to and impact... 50 Wildlife and Fisheries 10 2011-10-01 2011-10-01 false National Standard 4-Allocations. 600.325 Section 600.325 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND...

50 CFR 600.325 - National Standard 4-Allocations.

Code of Federal Regulations, 2014 CFR

2014-10-01

... communities, efficiency of various types of gear used in the fishery, transferability of effort to and impact... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false National Standard 4-Allocations. 600.325 Section 600.325 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND...

75 FR 6862 - Airworthiness Directives; Bombardier, Inc. Model CL-600-1A11 (CL-600), CL-600-2A12 (CL-601), CL...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-12

... hydraulic accumulator screw cap or end cap failure have been experienced on CL-600-2B19 (CRJ) aircraft... experienced on CL-600-2B19 (CRJ) aircraft, resulting in loss of the associated hydraulic system and high... on CL-600-2B19 (CRJ) aircraft, resulting in loss of the associated hydraulic system and high-energy...

Oncogenic B-Raf(V600E) abrogates the AKT/B-Raf/Mps1 interaction in melanoma cells.

PubMed

Zhang, Ling; Shi, Ruyi; He, Chanting; Cheng, Caixia; Song, Bin; Cui, Heyang; Zhang, Yanyan; Zhao, Zhiping; Bi, Yanghui; Yang, Xiaofeng; Miao, Xiaoping; Guo, Jiansheng; Chen, Xing; Wang, Jinfen; Li, Yaoping; Cheng, Xiaolong; Liu, Jing; Cui, Yongping

2013-08-28

Activating B-Raf mutations that deregulate the mitogen-activated protein kinase (MAPK) pathway commonly occur in cancer. Although B-Raf(V600E) induces increased Mps1 protein contributing to centrosome amplification and chromosome instability, the regulatory mechanisms of Mps1 in melanoma cells is not fully understood. Here, we report that Mps1/AKT and B-Raf(WT)/ERK signaling form an auto-regulatory negative feedback loop in melanoma cells; notably, oncogenic B-Raf(V600E) abrogates the negative feedback loop, contributing the aberrant Mps1 functions and tumorigenesis. Our findings raise the possibility that targeting the oncogenic B-Raf and Mps1, especially when used in combination could potentially provide great therapeutic opportunities for cancer treatment. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

77 FR 10409 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-22

...; Model A310 series airplanes; Model A300 B4-600, B4-600R, and F4-600R series airplanes, and Model C4-605R... Model A300 B4-603, B4-620, and B4-622 airplanes, Model A300 B4-605R and B4-622R airplanes, Model A300 F4-605R and F4- 622R airplanes, and Model A300 C4-605R Variant F airplanes. (d) Subject Air Transport...

77 FR 24367 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-24

... Airworthiness Directives; Airbus Airplanes AGENCY: Federal Aviation Administration (FAA), Department of... Airbus Model A300 B4-2C, B4-103, and B4-203 airplanes; Model A300 B4- 600, B4-600R, and F4-600R series airplanes, and Model C4-605R Variant F airplanes (collectively called A300-600 series airplanes); and Model...

42 CFR 436.600 - Scope.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 4 2011-10-01 2011-10-01 false Scope. 436.600 Section 436.600 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL... specified in subparts B, C, and D of this part. Subparts H and I of this part prescribe additional financial...

42 CFR 436.600 - Scope.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 4 2010-10-01 2010-10-01 false Scope. 436.600 Section 436.600 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL... specified in subparts B, C, and D of this part. Subparts H and I of this part prescribe additional financial...

75 FR 74665 - Airworthiness Directives; Airbus Model A310 Airplanes, and Airbus Model A300 B4-600, B4-600R, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-01

... landing gear retraction. * * * * * Uncommanded movement of the pilot and co-pilot seats during takeoff or... landing gear retraction. AD 2009-0084 required the deactivation of the electrical power of SOGERMA pilot... retraction. * * * * * Uncommanded movement of the pilot and co-pilot seats during takeoff or landing could...

Development of a novel class of B-RafV600E-selective inhibitors through virtual screening and hierarchical hit optimization

PubMed Central

Kong, Xiangqian; Qin, Jie; Li, Zeng; Vultur, Adina; Tong, Linjiang; Feng, Enguang; Rajan, Geena; Liu, Shien; Lu, Junyan; Liang, Zhongjie; Zheng, Mingyue; Zhu, Weiliang; Jiang, Hualiang; Herlyn, Meenhard; Liu, Hong; Marmorstein, Ronen; Luo, Cheng

2012-01-01

Oncogenic mutations in critical nodes of cellular signaling pathways have been associated with tumorigenesis and progression. The B-Raf protein kinase, a key hub in the canonical MAPK signaling cascade, is mutated in a broad range of human cancers and especially in malignant melanoma. The most prevalent B-RafV600E mutant exhibits elevated kinase activity and results in constitutive activation of the MAPK pathway, thus making it a promising drug target for cancer therapy. Herein, we described the development of novel B-RafV600E selective inhibitors via multi-step virtual screening and hierarchical hit optimization. Nine hit compounds with low micromolar IC50 values were identified as B-RafV600E inhibitors through virtual screening. Subsequent scaffold-based analogue searching and medicinal chemistry efforts significantly improved both the inhibitor potency and oncogene selectivity. In particular, compounds 22f and 22q possess nanomolar IC50 values with selectivity for B-RafV600E in vitro and exclusive cytotoxicity against B-RafV600E harboring cancer cells. PMID:22875039

Development of a novel class of B-Raf(V600E)-selective inhibitors through virtual screening and hierarchical hit optimization.

PubMed

Kong, Xiangqian; Qin, Jie; Li, Zeng; Vultur, Adina; Tong, Linjiang; Feng, Enguang; Rajan, Geena; Liu, Shien; Lu, Junyan; Liang, Zhongjie; Zheng, Mingyue; Zhu, Weiliang; Jiang, Hualiang; Herlyn, Meenhard; Liu, Hong; Marmorstein, Ronen; Luo, Cheng

2012-09-28

Oncogenic mutations in critical nodes of cellular signaling pathways have been associated with tumorigenesis and progression. The B-Raf protein kinase, a key hub in the canonical MAPK signaling cascade, is mutated in a broad range of human cancers and especially in malignant melanoma. The most prevalent B-Raf(V600E) mutant exhibits elevated kinase activity and results in constitutive activation of the MAPK pathway, thus making it a promising drug target for cancer therapy. Herein, we describe the development of novel B-Raf(V600E) selective inhibitors via multi-step virtual screening and hierarchical hit optimization. Nine hit compounds with low micromolar IC(50) values were identified as B-Raf(V600E) inhibitors through virtual screening. Subsequent scaffold-based analogue searching and medicinal chemistry efforts significantly improved both the inhibitor potency and oncogene selectivity. In particular, compounds 22f and 22q possess nanomolar IC(50) values with selectivity for B-Raf(V600E)in vitro and exclusive cytotoxicity against B-Raf(V600E) harboring cancer cells.

50 CFR 600.325 - National Standard 4-Allocations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... promote conservation (in the sense of wise use) by optimizing the yield in terms of size, value, market... Section 600.325 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND....325 National Standard 4—Allocations. (a) Standard 4. Conservation and management measures shall not...

78 FR 9787 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-12

... Airworthiness Directives; Airbus Airplanes AGENCY: Federal Aviation Administration (FAA), Department of... Airbus Model A300 B4-600, B4-600R, and F4-600R series airplanes, and Model A300 C4-605R Variant F airplanes (collectively called Model A300- 600 series airplanes); and Airbus [[Page 9788

A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells.

PubMed

Stuart, Scott A; Houel, Stephane; Lee, Thomas; Wang, Nan; Old, William M; Ahn, Natalie G

2015-06-01

Inhibitors of oncogenic B-RAF(V600E) and MKK1/2 have yielded remarkable responses in B-RAF(V600E)-positive melanoma patients. However, the efficacy of these inhibitors is limited by the inevitable onset of resistance. Despite the fact that these inhibitors target the same pathway, combination treatment with B-RAF(V600E) and MKK1/2 inhibitors has been shown to improve both response rates and progression-free survival in B-RAF(V600E) melanoma patients. To provide insight into the molecular nature of the combinatorial response, we used quantitative mass spectrometry to characterize the inhibitor-dependent phosphoproteome of human melanoma cells treated with the B-RAF(V600E) inhibitor PLX4032 (vemurafenib) or the MKK1/2 inhibitor AZD6244 (selumetinib). In three replicate experiments, we quantified changes at a total of 23,986 phosphosites on 4784 proteins. This included 1317 phosphosites that reproducibly decreased in response to at least one inhibitor. Phosphosites that responded to both inhibitors grouped into networks that included the nuclear pore complex, growth factor signaling, and transcriptional regulators. Although the majority of phosphosites were responsive to both inhibitors, we identified 16 sites that decreased only in response to PLX4032, suggesting rare instances where oncogenic B-RAF signaling occurs in an MKK1/2-independent manner. Only two phosphosites were identified that appeared to be uniquely responsive to AZD6244. When cells were treated with the combination of AZD6244 and PLX4032 at subsaturating concentrations (30 nm), responses at nearly all phosphosites were additive. We conclude that AZD6244 does not substantially widen the range of phosphosites inhibited by PLX4032 and that the benefit of the drug combination is best explained by their additive effects on suppressing ERK1/2 signaling. Comparison of our results to another recent ERK1/2 phosphoproteomics study revealed a surprising degree of variability in the sensitivity of phosphosites to

50 CFR 600.515 - Interpretation of 16 U.S.C. 1857(4).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Interpretation of 16 U.S.C. 1857(4). 600.515 Section 600.515 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MAGNUSON-STEVENS ACT PROVISIONS Foreign Fishing § 600.515...

10 CFR 600.20 - Maximum DOE obligation.

Code of Federal Regulations, 2011 CFR

2011-01-01

... DOE obligation. (a) The maximum DOE obligation to the recipient is— (1) For monetary awards, the amount shown in the award as the amount of DOE funds obligated, and (2) Any designated property. (b) DOE... 10 Energy 4 2011-01-01 2011-01-01 false Maximum DOE obligation. 600.20 Section 600.20 Energy...

10 CFR 600.20 - Maximum DOE obligation.

Code of Federal Regulations, 2013 CFR

2013-01-01

... DOE obligation. (a) The maximum DOE obligation to the recipient is— (1) For monetary awards, the amount shown in the award as the amount of DOE funds obligated, and (2) Any designated property. (b) DOE... 10 Energy 4 2013-01-01 2013-01-01 false Maximum DOE obligation. 600.20 Section 600.20 Energy...

10 CFR 600.20 - Maximum DOE obligation.

Code of Federal Regulations, 2012 CFR

2012-01-01

... DOE obligation. (a) The maximum DOE obligation to the recipient is— (1) For monetary awards, the amount shown in the award as the amount of DOE funds obligated, and (2) Any designated property. (b) DOE... 10 Energy 4 2012-01-01 2012-01-01 false Maximum DOE obligation. 600.20 Section 600.20 Energy...

10 CFR 600.20 - Maximum DOE obligation.

Code of Federal Regulations, 2014 CFR

2014-01-01

... DOE obligation. (a) The maximum DOE obligation to the recipient is— (1) For monetary awards, the amount shown in the award as the amount of DOE funds obligated, and (2) Any designated property. (b) DOE... 10 Energy 4 2014-01-01 2014-01-01 false Maximum DOE obligation. 600.20 Section 600.20 Energy...

Surgical implications of B-RafV600E mutation in fine-needle aspiration of thyroid nodules

PubMed Central

Mekel, Michal; Nucera, Carmelo; Hodin, Richard A.; Parangi, Sareh

2013-01-01

BACKGROUND Management of patients with thyroid nodules is based on establishing an accurate diagnosis; however, differentiating benign from malignant lesions preoperatively is not always possible using current cytological techniques. Novel molecular testing on cytological material could lead to clearer treatment algorithms. B-RafV600E mutation is the most common genetic alteration in thyroid cancer, specifically found in papillary thyroid cancer (PTC), and usually reported to be associated with aggressive disease. DATA SOURCE A literature search using PubMed identified all the pertinent literature on the identification and utilization of the B-RafV600E mutation in thyroid cancer. CONCLUSIONS The utility of using B-Raf mutation testing for nodules with indeterminate cytology is limited since many of those nodules (benign and malignant) do not harbor B-Raf mutations. However, when the pathologist sees cytological features suspicious for PTC, B-RafV600E mutation analysis may enhance the assessment of preoperative risks for PTC, directing a more aggressive initial surgical management when appropriate. PMID:20637346

76 FR 33658 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-09

... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440); Model CL-600-2C10 (Regional Jet Series 700, 701, & 702); Model CL-600-2D15 (Regional Jet Series 705); and Model CL-600-2D24 (Regional Jet Series 900) Airplanes AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of...

75 FR 47249 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-05

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010.... Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes, CL-600-2C10 (Regional Jet Series 700, 701, & 702) Airplanes, CL-600-2D15 (Regional Jet Series 705) Airplanes, and CL-600-2D24 (Regional Jet Series...

Production of a thermostable 1,3-1,4-β-glucanase mutant in Bacillus subtilis WB600 at a high fermentation capacity and its potential application in the brewing industry.

PubMed

Niu, Chengtuo; Liu, Chunfeng; Li, Yongxian; Zheng, Feiyun; Wang, Jinjing; Li, Qi

2018-02-01

1,3-1,4-β-glucanase was an important biotechnological aid in the brewing industry. In a previous research, a Bacillus BglTO mutant (BglTO) with high tolerance towards high temperature and low-pH conditions was constructed and expressed in Escherichia coli. However, E. coli was not a suitable host for enzyme production in food industry. Therefore, the present work aimed to achieve the high-level expression of BglTO in Bacillus subtilis WB600 and to test its effect in Congress mashing. The β-glucanase mutant was successfully expressed in B. subtilis WB600 and favorable plasmid segregation and structural stability were observed. The maximal extracellular activity of β-glucanase in recombinant B. subtilis WB600 reached 4840.4UmL -1 after cultivation condition optimization, which was 1.94-fold higher than that before optimization. The fermentation capacity of recombinant B. subtilis reached 242.02UmL -1 h -1 , which was the highest among all reported β-glucanases. The addition of BglTO in Congress mashing significantly reduced the filtration time and viscosity of mash by 29.7% and 12.3%, respectively, which was superior to two commercial enzymes. These favorable properties indicated that B. subtilis WB600 was a suitable host for production of BglTO, which was promising for application in the brewing industry. Copyright © 2017 Elsevier B.V. All rights reserved.

Identification of novel B-RafV600E inhibitors employing FBDD strategy.

PubMed

Wang, Peng-Fei; Qiu, Han-Yue; Wang, Ze-Feng; Zhang, Yong-Jiao; Wang, Zhong-Chang; Li, Dong-Dong; Zhu, Hai-Liang

2017-05-15

B-Raf kinase is the key point in a main branch of mitogen-activated protein kinase pathways and some of its mutations, such as the V600E mutation, lead to the persistent activation of ERK signaling and the trigger of severe diseases, including melanoma and other somatic cancers. Several potent drugs have been approved to treat B-Raf-related tumors, however, cases of resistance and relapse have been reported universally. Hence, differential scaffolds are in need to alleviate the scarcity of drugs and benefit the therapy of B-Raf-mutant cancers. Herein we report our recent work on the construction of novel B-Raf V600E inhibitors employing fragment-based drug design strategy. In this research, we decomposed known inhibitors to fragments and rebuilt new candidates using these blocks according to the evaluation of their potential. Lead compounds were synthesized after selection by means of virtual screening and molecular dynamics validation. Afterwards, we tested the pharmacological efficiency of these entities both in vitro and in vivo utilizing A375 xenograft model. The results favored our rational design intention and hinted this new kind of inhibitors might be helpful in the further explorations of potent agents. Copyright © 2017 Elsevier Inc. All rights reserved.

10 CFR 600.220 - Standards for financial management systems.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Standards for financial management systems. 600.220... Post-Award Requirements § 600.220 Standards for financial management systems. (a) A State must expend... prohibitions of applicable statutes. (b) The financial management systems of other grantees and subgrantees...

10 CFR 600.220 - Standards for financial management systems.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Standards for financial management systems. 600.220... Post-Award Requirements § 600.220 Standards for financial management systems. (a) A State must expend... prohibitions of applicable statutes. (b) The financial management systems of other grantees and subgrantees...

10 CFR 600.220 - Standards for financial management systems.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Standards for financial management systems. 600.220... Post-Award Requirements § 600.220 Standards for financial management systems. (a) A State must expend... prohibitions of applicable statutes. (b) The financial management systems of other grantees and subgrantees...

75 FR 12152 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440), CL...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-15

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010.... Model CL-600-2B19 (Regional Jet Series 100 & 440), CL-600-2C10 (Regional Jet Series 700, 701 & 702), CL-600-2D15 (Regional Jet Series 705), and CL-600-2D24 (Regional Jet Series 900) Airplanes AGENCY...

78 FR 9581 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-11

... Airworthiness Directives; Airbus Airplanes AGENCY: Federal Aviation Administration (FAA), Department of... Airbus Model A300 B4-600, B4-600R, and F4-600R series airplanes, and Model A300 C4-605R Variant F...-03-11 Airbus: Amendment 39-17346. Docket No. FAA-2012-1002; Directorate Identifier 2012-NM-052-AD. (a...

78 FR 15874 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-13

... Airworthiness Directives; Airbus Airplanes AGENCY: Federal Aviation Administration (FAA), Department of... Airbus Model A300 B4-600, B4-600R, and F4-600R series airplanes, and Model A300 C4-605R Variant F... ground threat avoidance, an Airbus A310 aeroplane experienced an uncommanded slide back of the co-pilot...

76 FR 63177 - Airworthiness Directives; Airbus Model A300 B2-1C, A300 B2-203, A300 B2K-3C, A300-B4-103, A300 B4...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-12

... available in the AD docket shortly after receipt. List of Subjects in 14 CFR Part 39 Air transportation... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2011... B4-2C Airplanes AGENCY: Federal Aviation Administration (FAA), Department of Transportation (DOT...

MicroRNA-300 promotes apoptosis and inhibits proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway by targeting CUL4B in pancreatic cancer cells.

PubMed

Zhang, Jia-Qiang; Chen, Shi; Gu, Jiang-Ning; Zhu, Yi; Zhan, Qian; Cheng, Dong-Feng; Chen, Hao; Deng, Xia-Xing; Shen, Bai-Yong; Peng, Cheng-Hong

2018-01-01

The study aims to verify the hypothesis that up-regulation of microRNA-300 (miR-300) targeting CUL4B promotes apoptosis and suppresses proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of pancreatic cancer cells by regulating the Wnt/β-catenin signaling pathway. Pancreatic cancer tissues and adjacent tissues were collected from 110 pancreatic cancer patients. Expression of miR-300, CUL4B, Wnt, β-catenin, E-cadherin, N-cadherin, Snail, GSK-3β, and CyclinD1 were detected using qRT-PCR and Western blot. CFPAC-1, Capan-1, and PANC-1 were classified into blank, negative control (NC), miR-300 mimics, miR-300 inhibitors, siRNA-CUL4B, and miR-300 inhibitors + siRNA-CUL4B groups. The proliferation, migration, invasion abilities, the cell cycle distribution, and apoptosis rates were measured in CCK-8 and Transwell assays. Pancreatic cancer tissues showed increased CUL4B expression but decreased miR-300 expression. When miR-300 was lowly expressed, CUL4B was upregulated which in-turn activated the Wnt/β-catenin pathway to protect the β-catenin expression and thus induce EMT. When miR-300 was highly expressed, CUL4B was downregulated which in-turn inhibited the Wnt/β-catenin pathway to prevent EMT. Weakened cell migration and invasion abilities and enhanced apoptosis were observed in the CUL4B group. The miR-300 inhibitors group exhibited an evident increase in growth rate accompanied the largest tumor volume. Smaller tumor volume and slower growth rate were observed in the miR-300 mimics and siRNA-CUL4B group. Our study concludes that lowly expressed miR-300 may contribute to highly expressed CUL4B activating the Wnt/β-catenin signaling pathway and further stimulating EMT, thus promoting proliferation and migration but suppressing apoptosis of pancreatic cancer cells. © 2017 Wiley Periodicals, Inc.

Basic design studies for a 600 MWe CFB boiler (270b, 2 x 600 C)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bursi, J.M.; Lafanechere, L.; Jestin, L.

Commercial CFB boilers are currently available in the 300 MWe equivalent range for use with international coal. Retrofitting of Provence 4 with a 250 MWe CFB boiler was an important step in CFB development. In light of the results obtained from two large French units--Emile Huchet 4 (125 MWe) and Provence 4 (250 MWe)--this paper focuses on the main technical points which are currently being studied in relation to the basic design of a 600 MWe CFB boiler, a project that has been undertaken by EDF. The general aim of this project is to demonstrate the competitiveness of a CFBmore » boiler compared with a PF boiler. The main areas of focus in the design of this large CFB boiler with advanced steam conditions are described. These points are subjected to particular analysis from a design standpoint. The objective is to prepare the precise specifications needed to ensure a product which is optimized in terms of quality/cost or service/cost. Due to the present lack of theoretical understanding of the refined and complex two-phase flow, design is a challenge which has to be based on reliable and comprehensive data obtained from large plants in commercial operation. This will ensure that the advantages of CFB which arise from the hydrodynamics within the circulation loop are maintained. The major goals of maintaining good particle residence time and concentration in the furnace are described. Misunderstanding of CFB furnace bottom conditions is also pointed out, with cost reduction and better NO{sub x} capture certainly among the major new targets in relation to bottom furnace design. General problems associated with the heat exchanger arrangement, principally those linked to high steam conditions and, especially, the vaporization system, are discussed. Once again, comparison with PF in this area showed that CFB boilers appear more competitive. Finally, the main area in which there is a need for sharing of CFB experience among CFB users is pointed out.« less

40 CFR 35.600 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-07-01

... ASSISTANCE Environmental Program Grants for Tribes Water Quality Cooperative Agreements (section 104(b)(3)) § 35.600 Purpose. (a) Purpose of section. Sections 35.600 through 35.604 govern Water Quality... Water Act. These sections do not govern Water Quality Cooperative Agreements under section 104(b)(3) to...

78 FR 70003 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-22

... Airworthiness Directives; Airbus Airplanes AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: We propose to adopt a new airworthiness directive (AD) for all Airbus Model A300 series airplanes; Airbus Model A300 B4-600, B4-600R, and F4-600R series airplanes, and Model...

77 FR 40485 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-10

... Airworthiness Directives; Airbus Airplanes AGENCY: Federal Aviation Administration (FAA), Department of... (AD) for all Airbus Model A300 series airplanes; all Model A300 B4-600, B4-600R, and F4-600R series... new AD: 2012-13-06 Airbus: Amendment 39-17108. Docket No. FAA-2012-0040; Directorate Identifier 2011...

A HRM assay for identification of low level BRAF V600E and V600K mutations using the CADMA principle in FFPE specimens.

PubMed

Huebner, Claudia; Weber, Remeny; Lloydd, Richard

2017-12-01

Melanoma patients with BRAF V600E and V600K mutations show complete or partial response to vemurafenib. Detection assays often scan for the common V600E mutation rather than the rare V600K variant, although this mutation can be found in a high proportion of melanoma patients in the South Pacific. Herein, we describe a BRAF high resolution melting (HRM) assay that can differentiate low level of V600E and V600K mutations using formalin fixed, paraffin embedded (FFPE) reference standards for assay validation. The assay is based on the competitive amplification of differentially melting amplicons (CADMA principle) and has a limit of detection of 0.8% mutant allele for V600K and 1.4% mutant allele for V600E. A differentiation between the two mutations based on the melting profile is possible even at low mutation level. Sixty FFPE specimens were scanned and mutations could be scored correctly as confirmed by castPCR. In summary, the developed HRM assay is suitable for detection of V600K and V600E mutations and proved to be reliable and cost effective in a diagnostic environment. Copyright © 2017 Royal College of Pathologists of Australasia. Published by Elsevier B.V. All rights reserved.

Qatar Exoplanet Survey : Qatar-3b, Qatar-4b, and Qatar-5b

NASA Astrophysics Data System (ADS)

Alsubai, Khalid; Mislis, Dimitris; Tsvetanov, Zlatan I.; Latham, David W.; Bieryla, Allyson; Buchhave, Lars A.; Esquerdo, Gilbert A.; Bramich, D. M.; Pyrzas, Stylianos; Vilchez, Nicolas P. E.; Mancini, Luigi; Southworth, John; Evans, Daniel F.; Henning, Thomas; Ciceri, Simona

2017-04-01

We report the discovery of Qatar-3b, Qatar-4b, and Qatar-5b, three new transiting planets identified by the Qatar Exoplanet Survey. The three planets belong to the hot Jupiter family, with orbital periods of {P}{{Q}3{{b}}} = 2.50792 days, {P}{{Q}4{{b}}} = 1.80539 days, and {P}{{Q}5{{b}}} = 2.87923 days. Follow-up spectroscopic observations reveal the masses of the planets to be {M}{{Q}3{{b}}} = 4.31 ± 0.47 {M}{{J}}, {M}{{Q}4{{b}}} = 6.10 ± 0.54 {M}{{J}}, and {M}{{Q}5{{b}}} = 4.32 ± 0.18 {M}{{J}}, while model fits to the transit light curves yield radii of {R}{{Q}3{{b}}} = 1.096 ± 0.14 {R}{{J}}, {R}{{Q}4{{b}}} = 1.135 ± 0.11 {R}{{J}}, and {R}{{Q}5{{b}}} = 1.107 ± 0.064 {R}{{J}}. The host stars are low-mass main sequence stars with masses and radii M Q3 = 1.145 ± 0.064 M ⊙, M Q4 = 0.896 ± 0.048 M ⊙, M Q5 = 1.128 ± 0.056 M ⊙ and R Q3 = 1.272 ± 0.14 R ⊙, R Q4 = 0.849 ± 0.063 R ⊙, and R Q5 = 1.076 ± 0.051 R ⊙ for Qatar-3, 4, and 5 respectively. The V magnitudes of the three host stars are V Q3 = 12.88, V Q4 = 13.60, and V Q5 = 12.82. All three new planets can be classified as heavy hot Jupiters (M > 4 M J).

B11 NMR in the layered diborides OsB2 and RuB2

NASA Astrophysics Data System (ADS)

Suh, B. J.; Zong, X.; Singh, Y.; Niazi, A.; Johnston, D. C.

2007-10-01

B11 nuclear magnetic resonance (NMR) measurements have been performed on B11 enriched OsB2 and RuB2 polycrystalline powder samples in an external field of 4.7T and in the temperature range, 4.2K300K . The spectra for both samples show similar quadrupole powder patterns that are typical for a nonaxial symmetry. The Knight shifts K in both samples are very small and constant in temperature. The nuclear spin-lattice relaxation rate T1-1 follows a Korringa law in the whole temperature range investigated with T1T=600 and 680sK for OsB2 and RuB2 , respectively. The experimental results indicate that a p character dominates the conduction electron wave function at the B site with a negligibly small s character in both compounds.

50 CFR 600.625 - Secretary's decision.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 10 2011-10-01 2011-10-01 false Secretary's decision. 600.625 Section 600... Section 306(b) § 600.625 Secretary's decision. (a) The Secretary will, on the basis of the hearing, record... for Federal preemption are determined to exist, the Secretary will notify in writing the Attorney...

50 CFR 600.625 - Secretary's decision.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Secretary's decision. 600.625 Section 600... Section 306(b) § 600.625 Secretary's decision. (a) The Secretary will, on the basis of the hearing, record... for Federal preemption are determined to exist, the Secretary will notify in writing the Attorney...

50 CFR 600.625 - Secretary's decision.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Secretary's decision. 600.625 Section 600... Section 306(b) § 600.625 Secretary's decision. (a) The Secretary will, on the basis of the hearing, record... for Federal preemption are determined to exist, the Secretary will notify in writing the Attorney...

46 CFR 199.600 - General.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false General. 199.600 Section 199.600 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) LIFESAVING APPLIANCES AND ARRANGEMENTS LIFESAVING SYSTEMS FOR.... This subpart sets out specific exemptions and alternatives to requirements in subparts A, B, C, D, and...

Continuous lake-sediment records of glaciation in the Sierra Nevada between 52,600 and 12,500 14C yr B.P.

USGS Publications Warehouse

Benson, L.V.; May, Howard M.; Antweiler, Ronald C.; Brinton, T.I.; Kashgarian, Michaele; Smoot, J.P.; Lund, S.P.

1998-01-01

The chemistry of the carbonate-free clay-size fraction of Owens Lake sediments supports the use of total organic carbon and magnetic susceptibility as indicators of stadial-interstadial oscillations. Owens Lake records of total organic carbon, magnetic susceptibility, and chemical composition of the carbonate-free, clay-size fraction indicate that Tioga glaciation began ~24,500 and ended by ~13,600 14C yr B.P. Many of the components of glacial rock flour (e.g., TiO2, MnO, BaO) found in Owens Lake sediments achieved maximum values during the Tioga glaciation when valley glaciers reached their greatest extent. Total organic carbon and SiO2 (amorphous) concentrations reached minimum values during Tioga glaciation, resulting from decreases in productivity that accompanied the introduction of rock flour into the surface waters of Owens Lake. At least 20 stadial-interstadial oscillations occurred in the Sierra Nevada between 52,600 and 14,000 14C yr B.P. Total organic carbon data from a Pyramid Lake sediment core also indicate oscillations in glacier activity between >39,500 and ~13,600 14C yr B.P. Alpine glacier oscillations occurred on a frequency of ???1900 yr in both basins, suggesting that millennial-scale oscillations occurred in California and Nevada during most of the past 52,600 yr.

75 FR 38009 - Airworthiness Directives; The Boeing Company Model 737-200, -300, -400, -500, -600, -700, -800...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-01

... Airworthiness Directives; The Boeing Company Model 737-200, -300, -400, -500, -600, -700, -800, and -900 Series Airplanes; Model 747-400 Series Airplanes; Model 757-200 and 757-300 Series Airplanes; Model 767-200, 767..., -500, -600, -700, -800, and -900 series airplanes; Model 747-400 series airplanes; Model 757-200 and...

Targeting BRAF V600E and Autophagy in Pediatric Brain Tumors

DTIC Science & Technology

2015-10-01

AWARD NUMBER: W81XWH-14-1-0414 TITLE: Targeting BRAF V600E and Autophagy in Pediatric Brain Tumors PRINCIPAL INVESTIGATOR: Jean Mulcahy...29 Sep 2015 4. TITLE AND SUBTITLE 5a. CONTRACT NUMBER W81XWH-14-1-0414 Targeting BRAF V600E and Autophagy in Pediatric Brain Tumors 5b. GRANT...ABSTRACT 200 words most significant findings 15. SUBJECT TERMS autophagy , BRAF, brain tumor. pediatric 16. SECURITY CLASSIFICATION OF: 17

12 CFR 600.10 - Service of Process.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Service of Process. 600.10 Section 600.10 Banks... Procedures for Service Upon the Farm Credit Administration § 600.10 Service of Process. (a) Except as... Administration Board, 1501 Farm Credit Drive, McLean, Virginia 22102-5090. (b) Service of process upon the...

21 CFR 73.600 - Turmeric.

Code of Federal Regulations, 2011 CFR

2011-04-01

... mixtures for coloring foods. (b) Uses and restrictions. Turmeric may be safely used for the coloring of... 21 Food and Drugs 1 2011-04-01 2011-04-01 false Turmeric. 73.600 Section 73.600 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL LISTING OF COLOR...

77 FR 64701 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-23

... Airworthiness Directives; Airbus Airplanes AGENCY: Federal Aviation Administration (FAA), Department of... Airbus Model A300 series airplanes; Model A310 series airplanes; and Model A300 B4-600, B4-600R, and F4... that the AD be effective after Airbus completes certifying the improved design for the fuel pump half...

Continuous Lake-Sediment Records of Glaciation in the Sierra Nevada between 52,600 and 12,500 14C yr B.P

NASA Astrophysics Data System (ADS)

Benson, Larry V.; May, Howard M.; Antweiler, Ronald C.; Brinton, Terry I.; Kashgarian, Michaele; Smoot, Joseph P.; Lund, Steve P.

1998-09-01

The chemistry of the carbonate-free clay-size fraction of Owens Lake sediments supports the use of total organic carbon and magnetic susceptibility as indicators of stadial-interstadial oscillations. Owens Lake records of total organic carbon, magnetic susceptibility, and chemical composition of the carbonate-free, clay-size fraction indicate that Tioga glaciation began ˜24,500 and ended by ˜13,600 14C yr B.P. Many of the components of glacial rock flour (e.g., TiO 2, MnO, BaO) found in Owens Lake sediments achieved maximum values during the Tioga glaciation when valley glaciers reached their greatest extent. Total organic carbon and SiO 2(amorphous) concentrations reached minimum values during Tioga glaciation, resulting from decreases in productivity that accompanied the introduction of rock flour into the surface waters of Owens Lake. At least 20 stadial-interstadial oscillations occurred in the Sierra Nevada between 52,600 and 14,000 14C yr B.P. Total organic carbon data from a Pyramid Lake sediment core also indicate oscillations in glacier activity between >39,500 and ˜13,600 14C yr B.P. Alpine glacier oscillations occurred on a frequency of ≤1900 yr in both basins, suggesting that millennial-scale oscillations occurred in California and Nevada during most of the past 52,600 yr.

50 CFR 600.1012 - Reduction loan.

Code of Federal Regulations, 2010 CFR

2010-10-01

... annual rate, that shall be determined by subsequent events, of simple interest on the reduction loan's... deposit and disburse the fee revenue in accordance with § 600.1014. (b) Principal amount, interest rate... § 600.1014. (c) Effect of prospective interest rate. Any difference between a prospective interest rate...

Measurements of cross section of e + e - → p p ¯ π 0 at center-of-mass energies between 4.008 and 4.600 GeV

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ablikim, M.; Achasov, M. N.; Ahmed, S.

Based on e +e - annihilation data samples collected with the BESIII detector at the BEPCII collider at 13 center-of-mass energies from 4.008 to 4.600 GeV, measurements of the Born cross section of e +e -→more » $$p\\bar{p}$$π 0 are then performed. No significant resonant structure is observed in the measured energy dependence of the cross section. The upper limit on the Born cross section of e +e -→Y (4260) →$$p\\bar{p}$$π 0 at the 90% C.L. is determined to be 0.01 pb. The upper limit on the ratio of the branching fractions B(Y(4260)→$$p\\bar{p}$$π 0) / B(Y(4260)→π +π - J/ψ) at the 90% C.L. is determined to be 0.02%.« less

Measurements of cross section of e + e - → p p ¯ π 0 at center-of-mass energies between 4.008 and 4.600 GeV

DOE PAGES

Ablikim, M.; Achasov, M. N.; Ahmed, S.; ...

2017-08-10

Based on e +e - annihilation data samples collected with the BESIII detector at the BEPCII collider at 13 center-of-mass energies from 4.008 to 4.600 GeV, measurements of the Born cross section of e +e -→more » $$p\\bar{p}$$π 0 are then performed. No significant resonant structure is observed in the measured energy dependence of the cross section. The upper limit on the Born cross section of e +e -→Y (4260) →$$p\\bar{p}$$π 0 at the 90% C.L. is determined to be 0.01 pb. The upper limit on the ratio of the branching fractions B(Y(4260)→$$p\\bar{p}$$π 0) / B(Y(4260)→π +π - J/ψ) at the 90% C.L. is determined to be 0.02%.« less

COX-2 Elevates Oncogenic miR-526b in Breast Cancer by EP4 Activation.

PubMed

Majumder, Mousumi; Landman, Erin; Liu, Ling; Hess, David; Lala, Peeyush K

2015-06-01

MicroRNAs (miRs) are small regulatory molecules emerging as potential biomarkers in cancer. Previously, it was shown that COX-2 expression promotes breast cancer progression via multiple mechanisms, including induction of stem-like cells (SLC), owing to activation of the prostaglandin E2 receptor EP4 (PTGER4). COX-2 overexpression also upregulated microRNA-526b (miR-526b), in association with aggressive phenotype. Here, the functional roles of miR-526b in breast cancer and the mechanistic role of EP4 signaling in miR-526b upregulation were examined. A positive correlation was noted between miR-526b and COX-2 mRNA expression in COX-2 disparate breast cancer cell lines. Stable overexpression of miR-526b in poorly metastatic MCF7 and SKBR3 cell lines resulted in increased cellular migration, invasion, EMT phenotype and enhanced tumorsphere formation in vitro, and lung colony formation in vivo in immunodeficient mice. Conversely, knockdown of miR-526b in aggressive MCF7-COX-2 and SKBR3-COX-2 cells reduced oncogenic functions and reversed the EMT phenotype, in vitro. Furthermore, it was determined that miR-526b expression is dependent on EP4 receptor activity and downstream PI3K-AKT and cyclic AMP (cAMP) signaling pathways. PI3K-AKT inhibitors blocked EP4 agonist-mediated miR-526b upregulation and tumorsphere formation in MCF7 and SKBR3 cells. NF-κB inhibitor abrogates EP agonist-stimulated miRNA expression in MCF7 and T47D cells, indicating that the NF-κB pathway is also involved in miR-526b regulation. In addition, inhibition of COX-2, EP4, PI3K, and PKA in COX-2-overexpressing cells downregulated miR-526b and its functions in vitro. Finally, miR-526b expression was significantly higher in cancerous than in noncancerous breast tissues and associated with reduced patient survival. In conclusion, miR-526b promotes breast cancer progression, SLC-phenotype through EP4-mediated signaling, and correlates with breast cancer patient survival. This study presents novel

10 CFR 600.143 - Competition.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Competition. 600.143 Section 600.143 Energy DEPARTMENT OF... Nonprofit Organizations Post-Award Requirements § 600.143 Competition. All procurement transactions shall be conducted in a manner to provide, to the maximum extent practical, open and free competition. The recipient...

10 CFR 600.316 - Audits.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Audits. 600.316 Section 600.316 Energy DEPARTMENT OF... Grants and Cooperative Agreements With For-Profit Organizations Post-Award Requirements § 600.316 Audits. (a) Any recipient that expends $500,000 or more in a year under Federal awards must have an audit...

50 CFR 600.520 - Northwest Atlantic Ocean fishery.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Northwest Atlantic Ocean fishery. 600.520... Northwest Atlantic Ocean fishery. (a) Purpose. Sections 600.520 and 600.525 regulate all foreign fishing conducted under a GIFA within the EEZ in the Atlantic Ocean north of 35°00′ N. lat. (b) Authorized fishery...

50 CFR 600.520 - Northwest Atlantic Ocean fishery.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Northwest Atlantic Ocean fishery. 600.520... Northwest Atlantic Ocean fishery. (a) Purpose. Sections 600.520 and 600.525 regulate all foreign fishing conducted under a GIFA within the EEZ in the Atlantic Ocean north of 35°00′ N. lat. (b) Authorized fishery...

50 CFR 600.520 - Northwest Atlantic Ocean fishery.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Northwest Atlantic Ocean fishery. 600.520... Northwest Atlantic Ocean fishery. (a) Purpose. Sections 600.520 and 600.525 regulate all foreign fishing conducted under a GIFA within the EEZ in the Atlantic Ocean north of 35°00′ N. lat. (b) Authorized fishery...

50 CFR 600.520 - Northwest Atlantic Ocean fishery.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 10 2011-10-01 2011-10-01 false Northwest Atlantic Ocean fishery. 600.520... Northwest Atlantic Ocean fishery. (a) Purpose. Sections 600.520 and 600.525 regulate all foreign fishing conducted under a GIFA within the EEZ in the Atlantic Ocean north of 35°00′ N. lat. (b) Authorized fishery...

50 CFR 600.133 - Scientific and Statistical Committee (SSC).

Code of Federal Regulations, 2013 CFR

2013-10-01

... information as is relevant to such Council's development and amendment of any fishery management plan. (b...). 600.133 Section 600.133 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC... Fishery Management Councils § 600.133 Scientific and Statistical Committee (SSC). (a) Each Council shall...

50 CFR 600.133 - Scientific and Statistical Committee (SSC).

Code of Federal Regulations, 2012 CFR

2012-10-01

... information as is relevant to such Council's development and amendment of any fishery management plan. (b...). 600.133 Section 600.133 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC... Fishery Management Councils § 600.133 Scientific and Statistical Committee (SSC). (a) Each Council shall...

Protective Effect of Zingiber Officinale against CCl4-Induced Liver Fibrosis Is Mediated through Downregulating the TGF-β1/Smad3 and NF-ĸB/IĸB Pathways.

PubMed

Hasan, Iman H; El-Desouky, M A; Hozayen, Walaa G; Abd el Aziz, Ghada M

2016-01-01

No ideal hepatoprotective agents are available in modern medicine to effectively prevent liver disorders. In this study, we aimed at evaluating the potential of Zingiber officinale in the regression of liver fibrosis and its underlining mechanism of action. To induce liver fibrosis, male Wistar rats received CCl4 (2 ml/kg/2 times/week; i.p.), with and without 300 or 600 mg/kg Z. officinale extract daily through oral gavage. To assess the protective effect of Z. officinale, liver function parameters, histopathology, inflammatory markers and gene expression of transforming growth factor-beta 1 (TGF-β1)/Smad3 and nuclear factor-kappa B (NF-ĸB)/IĸB pathways were analyzed. Results demonstrate that Z. officinale extract markedly prevented liver injury as evident by the decreased liver marker enzymes. Concurrent administration of Z. officinale significantly protected against the CCl4-induced inflammation as showed by the decreased pro-inflammatory cytokine levels as well as the downregulation of the NF-ĸB)/IĸB and TGF-β1/Smad3 pathways in CCl4-administered rats. In conclusion, our study provides evidence that the protective effect of Z. officinale against rat liver fibrosis could be explained through its ability to modulate the TGF-β1/Smad3 and NF-ĸB)/IĸB signaling pathways. © 2015 S. Karger AG, Basel.

75 FR 61348 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-05

... CFR Part 39 Air transportation, Aircraft, Aviation safety, Incorporation by reference, Safety... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY...

10 CFR 600.152 - Financial reporting.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Financial reporting. 600.152 Section 600.152 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform Administrative... Nonprofit Organizations Post-Award Requirements § 600.152 Financial reporting. (a) The following forms or...

10 CFR 603.600 - Administrative matters.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Administrative matters. 603.600 Section 603.600 Energy... Affecting Participants' Financial, Property, and Purchasing Systems § 603.600 Administrative matters. This subpart addresses “systemic” administrative matters that place requirements on the operation of a...

10 CFR 603.600 - Administrative matters.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Administrative matters. 603.600 Section 603.600 Energy... Affecting Participants' Financial, Property, and Purchasing Systems § 603.600 Administrative matters. This subpart addresses “systemic” administrative matters that place requirements on the operation of a...

10 CFR 603.600 - Administrative matters.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Administrative matters. 603.600 Section 603.600 Energy... Affecting Participants' Financial, Property, and Purchasing Systems § 603.600 Administrative matters. This subpart addresses “systemic” administrative matters that place requirements on the operation of a...

10 CFR 603.600 - Administrative matters.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Administrative matters. 603.600 Section 603.600 Energy... Affecting Participants' Financial, Property, and Purchasing Systems § 603.600 Administrative matters. This subpart addresses “systemic” administrative matters that place requirements on the operation of a...

10 CFR 603.600 - Administrative matters.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Administrative matters. 603.600 Section 603.600 Energy... Affecting Participants' Financial, Property, and Purchasing Systems § 603.600 Administrative matters. This subpart addresses “systemic” administrative matters that place requirements on the operation of a...

10 CFR 600.152 - Financial reporting.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Financial reporting. 600.152 Section 600.152 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform Administrative... Nonprofit Organizations Post-Award Requirements § 600.152 Financial reporting. (a) The following forms or...

10 CFR 600.31 - Research misconduct.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Research misconduct. 600.31 Section 600.31 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES General § 600.31 Research misconduct. (a) A recipient is responsible for maintaining the integrity of research of any kind under an...

10 CFR 600.31 - Research misconduct.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Research misconduct. 600.31 Section 600.31 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES General § 600.31 Research misconduct. (a) A recipient is responsible for maintaining the integrity of research of any kind under an...

10 CFR 600.31 - Research misconduct.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Research misconduct. 600.31 Section 600.31 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES General § 600.31 Research misconduct. (a) A recipient is responsible for maintaining the integrity of research of any kind under an...

10 CFR 600.31 - Research misconduct.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Research misconduct. 600.31 Section 600.31 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES General § 600.31 Research misconduct. (a) A recipient is responsible for maintaining the integrity of research of any kind under an...

75 FR 11422 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-11

... Part 39 Air transportation, Aircraft, Aviation safety, Incorporation by reference, Safety. Adoption of... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2009... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY...

75 FR 19203 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-14

... transportation, Aircraft, Aviation safety, Incorporation by reference, Safety. Adoption of the Amendment 0... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2009... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY...

76 FR 71241 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-17

..., Aircraft, Aviation safety, Incorporation by reference, Safety. Adoption of the Amendment Accordingly, under... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2011... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY...

75 FR 10667 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-09

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY: Federal Aviation Administration (FAA), Department of Transportation (DOT). ACTION: Final rule; request for...

75 FR 52233 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-25

... transportation, Aircraft, Aviation safety, Incorporation by reference, Safety. Adoption of the Amendment 0... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY...

76 FR 29997 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-24

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule; correction. SUMMARY: The FAA is correcting...

76 FR 38065 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-29

.... Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY: Federal Aviation Administration (FAA... Part 39 Air transportation, Aircraft, Aviation safety, Incorporation by reference, Safety. The Proposed... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2011...

75 FR 31324 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-03

.... Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY: Federal Aviation Administration (FAA... Part 39 Air transportation, Aircraft, Aviation safety, Incorporation by reference, Safety. The Proposed... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010...

10 CFR 600.148 - Contract provisions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Contract provisions. 600.148 Section 600.148 Energy... Nonprofit Organizations Post-Award Requirements § 600.148 Contract provisions. The recipient shall include... contracts. The following provisions shall also be applied to subcontracts. (a) Contracts in excess of the...

10 CFR 600.131 - Insurance coverage.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Insurance coverage. 600.131 Section 600.131 Energy... Nonprofit Organizations Post-Award Requirements § 600.131 Insurance coverage. Recipients shall, at a minimum, provide the equivalent insurance coverage for real property and equipment acquired with DOE funds as...

Association of BRAFV600E Mutation and MicroRNA Expression with Central Lymph Node Metastases in Papillary Thyroid Cancer: A Prospective Study from Four Endocrine Surgery Centers

PubMed Central

Aragon Han, Patricia; Kim, Hyun-seok; Cho, Soonweng; Fazeli, Roghayeh; Najafian, Alireza; Khawaja, Hunain; McAlexander, Melissa; Dy, Benzon; Sorensen, Meredith; Aronova, Anna; Sebo, Thomas J.; Giordano, Thomas J.; Fahey, Thomas J.; Thompson, Geoffrey B.; Gauger, Paul G.; Somervell, Helina; Bishop, Justin A.; Eshleman, James R.; Schneider, Eric B.; Witwer, Kenneth W.; Umbricht, Christopher B.

2016-01-01

Background: Studies have demonstrated an association of the BRAFV600E mutation and microRNA (miR) expression with aggressive clinicopathologic features in papillary thyroid cancer (PTC). Analysis of BRAFV600E mutations with miR expression data may improve perioperative decision making for patients with PTC, specifically in identifying patients harboring central lymph node metastases (CLNM). Methods: Between January 2012 and June 2013, 237 consecutive patients underwent total thyroidectomy and prophylactic central lymph node dissection (CLND) at four endocrine surgery centers. All tumors were tested for the presence of the BRAFV600E mutation and miR-21, miR-146b-3p, miR-146b-5p, miR-204, miR-221, miR-222, and miR-375 expression. Bivariate and multivariable analyses were performed to examine associations between molecular markers and aggressive clinicopathologic features of PTC. Results: Multivariable logistic regression analysis of all clinicopathologic features found miR-146b-3p and miR-146b-5p to be independent predictors of CLNM, while the presence of BRAFV600E almost reached significance. Multivariable logistic regression analysis limited to only predictors available preoperatively (molecular markers, age, sex, and tumor size) found miR-146b-3p, miR-146b-5p, miR-222, and BRAFV600E mutation to predict CLNM independently. While BRAFV600E was found to be associated with CLNM (48% mutated in node-positive cases vs. 28% mutated in node-negative cases), its positive and negative predictive values (48% and 72%, respectively) limit its clinical utility as a stand-alone marker. In the subgroup analysis focusing on only classical variant of PTC cases (CVPTC), undergoing prophylactic lymph node dissection, multivariable logistic regression analysis found only miR-146b-5p and miR-222 to be independent predictors of CLNM, while BRAFV600E was not significantly associated with CLNM. Conclusion: In the patients undergoing prophylactic CLNDs, miR-146b-3p, miR-146b-5p, and miR

40 CFR 600.107-78 - Fuel specifications.

Code of Federal Regulations, 2010 CFR

2010-07-01

... and Later Model Year Automobiles-Test Procedures § 600.107-78 Fuel specifications. (a) The test fuel... test fuel specifications for diesel automobiles are given in paragraphs (b) (1) and (2) of § 86.113 of... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Fuel specifications. 600.107-78...

75 FR 69609 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-15

.... Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY: Federal Aviation Administration (FAA... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010... airworthiness information (MCAI) originated by an aviation authority of another country to identify and correct...

75 FR 25788 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-10

.... Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY: Federal Aviation Administration (FAA.... List of Subjects in 14 CFR Part 39 Air transportation, Aircraft, Aviation safety, Incorporation by... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010...

75 FR 37708 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-30

.... List of Subjects in 14 CFR Part 39 Air transportation, Aircraft, Aviation safety, Incorporation by... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2009... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY...

75 FR 64636 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-20

... docket. List of Subjects in 14 CFR Part 39 Air transportation, Aircraft, Aviation safety, Incorporation... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY...

75 FR 63054 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-14

.... List of Subjects in 14 CFR Part 39 Air transportation, Aircraft, Aviation safety, Incorporation by... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2009... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY...

75 FR 22521 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-29

... after receipt. List of Subjects in 14 CFR Part 39 Air transportation, Aircraft, Aviation safety... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2009... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY...

75 FR 17086 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 & 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-05

.... Model CL-600-2B19 (Regional Jet Series 100 & 440) Airplanes AGENCY: Federal Aviation Administration (FAA... the AD docket. List of Subjects in 14 CFR Part 39 Air transportation, Aircraft, Aviation safety... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010...

10 CFR 600.141 - Recipient responsibilities.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Nonprofit Organizations Post-Award Requirements § 600.141 Recipient responsibilities. The standards... contract(s). The recipient is the responsible authority, without recourse to DOE regarding the settlement... 10 Energy 4 2012-01-01 2012-01-01 false Recipient responsibilities. 600.141 Section 600.141 Energy...

10 CFR 600.141 - Recipient responsibilities.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Nonprofit Organizations Post-Award Requirements § 600.141 Recipient responsibilities. The standards... contract(s). The recipient is the responsible authority, without recourse to DOE regarding the settlement... 10 Energy 4 2011-01-01 2011-01-01 false Recipient responsibilities. 600.141 Section 600.141 Energy...

10 CFR 600.141 - Recipient responsibilities.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Nonprofit Organizations Post-Award Requirements § 600.141 Recipient responsibilities. The standards... contract(s). The recipient is the responsible authority, without recourse to DOE regarding the settlement... 10 Energy 4 2013-01-01 2013-01-01 false Recipient responsibilities. 600.141 Section 600.141 Energy...

10 CFR 600.141 - Recipient responsibilities.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Nonprofit Organizations Post-Award Requirements § 600.141 Recipient responsibilities. The standards... contract(s). The recipient is the responsible authority, without recourse to DOE regarding the settlement... 10 Energy 4 2014-01-01 2014-01-01 false Recipient responsibilities. 600.141 Section 600.141 Energy...

10 CFR 600.124 - Program income.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Program income. 600.124 Section 600.124 Energy DEPARTMENT... Nonprofit Organizations Post-Award Requirements § 600.124 Program income. (a) The standards set forth in this section shall be used to account for program income related to projects financed in whole or in...

A 500-600 MHz GaN power amplifier with RC-LC stability network

NASA Astrophysics Data System (ADS)

Ma, Xinyu; Duan, Baoxing; Yang, Yintang

2017-08-01

A 500-600 MHz high-efficiency, high-power GaN power amplifier is designed and realized on the basis of the push-pull structure. The RC-LC stability network is proposed and applied to the power amplifier circuit for the first time. The RC-LC stability network can significantly reduce the high gain out the band, which eliminates the instability of the power amplifier circuit. The developed power amplifier exhibits 58.5 dBm (700 W) output power with a 17 dB gain and 85% PAE at 500-600 MHz, 300 μs, 20% duty cycle. It has the highest PAE in P-band among the products at home and abroad. Project supported by the National Key Basic Research Program of China (No. 2014CB339901).

10 CFR 600.132 - Real property.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Real property. 600.132 Section 600.132 Energy DEPARTMENT... Nonprofit Organizations Post-Award Requirements § 600.132 Real property. Unless otherwise provided by statute or program regulations, the requirements concerning the use and disposition of real property...

10 CFR 600.132 - Real property.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Real property. 600.132 Section 600.132 Energy DEPARTMENT... Nonprofit Organizations Post-Award Requirements § 600.132 Real property. Unless otherwise provided by statute or program regulations, the requirements concerning the use and disposition of real property...

10 CFR 600.132 - Real property.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Real property. 600.132 Section 600.132 Energy DEPARTMENT... Nonprofit Organizations Post-Award Requirements § 600.132 Real property. Unless otherwise provided by statute or program regulations, the requirements concerning the use and disposition of real property...

10 CFR 600.132 - Real property.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Real property. 600.132 Section 600.132 Energy DEPARTMENT... Nonprofit Organizations Post-Award Requirements § 600.132 Real property. Unless otherwise provided by statute or program regulations, the requirements concerning the use and disposition of real property...

B4GALT3 up-regulation by miR-27a contributes to the oncogenic activity in human cervical cancer cells.

PubMed

Sun, Yanrui; Yang, Xi; Liu, Min; Tang, Hua

2016-06-01

β-1,4-Galactosyltransferase III (B4GALT3) is an enzyme responsible for the generation of poly-N-acetyllactosamine and is involved in tumorigenesis. However, B4GALT3-dysregulation and its role in cervical cancer cells are unknown. Herein, we found that B4GALT3 was upregulated in cervical cancer tissues compared to adjacent non-tumor tissues. B4GALT3-overexpression promoted, whereas B4GALT3-knockdown suppressed the cellular migration, invasion and EMT of HeLa and C33A cervical cancer cells. To explore the mechanism of dysregulation, B4GALT3 was predicted to be a target of miR-27a. EGFP and pGL3-promoter reporter assay showed miR-27a binds to B4GALT3 3'UTR region but enhanced its expression. RT-qPCR showed miR-27a was also upregulated and presented positive correlation with B4GALT3-expression in cervical cancer tissues. miR-27a-overexpression promoted, but blocking-miR-27a repressed these malignancies in HeLa and C33A cells. Furthermore, shR-B4GALT3 counteracted the promotion of malignancies induced by miR-27a, suggesting miR-27a upregulates B4GALT3 to enhance tumorigenic activities. In addition, we found that B4GALT3 significantly enhances β1-integrin stability, thus mediating promotion of B4GALT3 on malignancy in cervical cancer cells. Altogether, our findings evidenced that B4GALT3 upregulated by miR-27a contributes to the tumorigenic activities by β1-integrin pathway and might provide potential biomarkers for cervical cancer. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

PSMB4 promotes multiple myeloma cell growth by activating NF-κB-miR-21 signaling

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zheng, Peihao; Guo, Honggang; Li, Guangchao

2015-03-06

Proteasomal subunit PSMB4, was recently identified as potential cancer driver genes in several tumors. However, the regulatory mechanism of PSMB4 on carcinogenesis process remains unclear. In this study, we investigated the expression and roles of PSMB4 in multiple myeloma (MM). We found a significant up-regulation of PSMB4 in MM plasma and cell lines. Ectopic overexpression of PSMB4 promoted cell growth and colony forming ability of MM cells, whereas inhibition of PSMB4 led to a decrease of such events. Furthermore, our results demonstrated the up-regulation of miR-21 and a positive correlation between the levels of miR-21 and PSMB4 in MM. Re-expressionmore » of miR-21 markedly rescued PSMB4 knockdown-mediated suppression of cell proliferation and clone-formation. Additionally, while enforced expression of PSMB4 profoundly increased NF-κB activity and the level of miR-21, PSMB4 knockdown or NF-κB inhibition suppressed miR-21 expression in MM cells. Taken together, our results demonstrated that PSMB4 regulated MM cell growth in part by activating NF-κB-miR-21 signaling, which may represent promising targets for novel specific therapies. - Highlights: • First reported upregulation of PSMB4 in MM plasma and cell lines. • PSMB4 promoted MM cell growth and colony forming ability. • Further found miR-21 was up-regulated by PSMB4 in MM plasma and cell lines. • PSMB4-induced miR-21 expression was modulated by NF-κB. • PSMB4-NF-κB-miR-21 axis may be potential therapeutic targets of MM.« less

10 CFR 600.244 - Termination for convenience.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Termination for convenience. 600.244 Section 600.244 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform... Requirements § 600.244 Termination for convenience. Except as provided in § 600.443 awards may be terminated in...

46 CFR 118.600 - Fire axe.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Fire axe. 118.600 Section 118.600 Shipping COAST GUARD... OVERNIGHT ACCOMMODATIONS FOR MORE THAN 49 PASSENGERS FIRE PROTECTION EQUIPMENT Additional Equipment § 118.600 Fire axe. A vessel of more than 19.8 meters (65 feet) in length must have at least one fire axe...

Reactivation of Mitogen-activated Protein Kinase (MAPK) Pathway by FGF Receptor 3 (FGFR3)/Ras Mediates Resistance to Vemurafenib in Human B-RAF V600E Mutant Melanoma*

PubMed Central

Yadav, Vipin; Zhang, Xiaoyi; Liu, Jiangang; Estrem, Shawn; Li, Shuyu; Gong, Xue-Qian; Buchanan, Sean; Henry, James R.; Starling, James J.; Peng, Sheng-Bin

2012-01-01

Oncogenic B-RAF V600E mutation is found in 50% of melanomas and drives MEK/ERK pathway and cancer progression. Recently, a selective B-RAF inhibitor, vemurafenib (PLX4032), received clinical approval for treatment of melanoma with B-RAF V600E mutation. However, patients on vemurafenib eventually develop resistance to the drug and demonstrate tumor progression within an average of 7 months. Recent reports indicated that multiple complex and context-dependent mechanisms may confer resistance to B-RAF inhibition. In the study described herein, we generated B-RAF V600E melanoma cell lines of acquired-resistance to vemurafenib, and investigated the underlying mechanism(s) of resistance. Biochemical analysis revealed that MEK/ERK reactivation through Ras is the key resistance mechanism in these cells. Further analysis of total gene expression by microarray confirmed a significant increase of Ras and RTK gene signatures in the vemurafenib-resistant cells. Mechanistically, we found that the enhanced activation of fibroblast growth factor receptor 3 (FGFR3) is linked to Ras and MAPK activation, therefore conferring vemurafenib resistance. Pharmacological or genetic inhibition of the FGFR3/Ras axis restored the sensitivity of vemurafenib-resistant cells to vemurafenib. Additionally, activation of FGFR3 sufficiently reactivated Ras/MAPK signaling and conferred resistance to vemurafenib in the parental B-RAF V600E melanoma cells. Finally, we demonstrated that vemurafenib-resistant cells maintain their addiction to the MAPK pathway, and inhibition of MEK or pan-RAF activities is an effective therapeutic strategy to overcome acquired-resistance to vemurafenib. Together, we describe a novel FGFR3/Ras mediated mechanism for acquired-resistance to B-RAF inhibition. Our results have implications for the development of new therapeutic strategies to improve the outcome of patients with B-RAF V600E melanoma. PMID:22730329

The thermal expansion and thermophysical properties of an aluminum and Al/B4C composite

NASA Astrophysics Data System (ADS)

Gladkovsky, S. V.; Kamantsev, I. S.; Kuteneva, S. V.; Veselova, V. E.; Ryzhkov, M. A.

2017-12-01

The paper presents results of experimental studies of the thermal expansion and thermophysical properties of an Al/B4C composite with a boron carbide content of 20 wt% and technically pure aluminum in the temperature range from 100 to 600°C to evaluate the possible use of this composite as a neutron-protective material in the nuclear industry.

10 CFR 600.353 - Disputes and appeals.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Disputes and appeals. 600.353 Section 600.353 Energy... Requirements for Grants and Cooperative Agreements With For-Profit Organizations Post-Award Requirements § 600.353 Disputes and appeals. Consistent with 10 CFR 600.22 and part 1024, recipients have the right to...

High-Resolution Synchrotron Infrared Spectroscopy of Thiophosgene: the νb{1}, νb{5}, 2νb{4}, and νb{2} + 2νb{6} bands

NASA Astrophysics Data System (ADS)

McKellar, Bob; Billinghurst, Brant E.

2015-06-01

Thiophosgene (Cl_2CS) is a favorite model system for studies of photophysics, vibrational dynamics, and intersystem interactions. But at high resolution its infrared spectrum is very congested due to hot bands and multiple isotopic species. Previously, we reported the first high resolution IR study of this molecule, analyzing the νb{2} (504 wn) and νb{4} (471 wn) fundamental bands. Here we continue, with analysis of the νb{1} (1139 wn) and νb{5} (820 wn) fundamentals for the two most abundant isotopologues, 35Cl2CS and 35Cl37ClCS, based on spectra with a resolution of about 0.001 wn obtained at the Canadian Light Source far-infrared beamline using synchrotron radiation and a Bruker IFS125 Fourier transform spectrometer. The νb{2} + νb{4} (942 wn) and νb{2} + 2νb{6} (1104 wn) bands are also studied here. But so far the νb{2} + νb{6} combination band (795 wn) resists analysis, as do the weak νb{3} (292.9 wn) and νb{6} (≈300? wn) fundamentals. A.R.W. McKellar, B.E.Billinghurst, J. Mol. Spectrosc. 260, 66 (2010).

34 CFR 82.600 - Semi-annual compilation.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 1 2010-07-01 2010-07-01 false Semi-annual compilation. 82.600 Section 82.600 Education Office of the Secretary, Department of Education NEW RESTRICTIONS ON LOBBYING Agency Reports § 82... reports (see appendix B) and, on May 31 and November 30 of each year, submit to the Secretary of the...

50 CFR 600.1003 - Content of a request for a financed program.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Content of a request for a financed program. 600.1003 Section 600.1003 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL... financed program shall: (a) Specify the reduction fishery. (b) Project the amount of the reduction and...

50 CFR 600.1005 - Content of a request for a subsidized program.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Content of a request for a subsidized program. 600.1005 Section 600.1005 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL... subsidized program shall: (a) Specify the reduction fishery. (b) Project the amount of the reduction and...

50 CFR 600.1005 - Content of a request for a subsidized program.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Content of a request for a subsidized program. 600.1005 Section 600.1005 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL... subsidized program shall: (a) Specify the reduction fishery. (b) Project the amount of the reduction and...

50 CFR 600.1005 - Content of a request for a subsidized program.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Content of a request for a subsidized program. 600.1005 Section 600.1005 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL... subsidized program shall: (a) Specify the reduction fishery. (b) Project the amount of the reduction and...

50 CFR 600.1005 - Content of a request for a subsidized program.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 10 2011-10-01 2011-10-01 false Content of a request for a subsidized program. 600.1005 Section 600.1005 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL... subsidized program shall: (a) Specify the reduction fishery. (b) Project the amount of the reduction and...

50 CFR 600.1003 - Content of a request for a financed program.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 50 Wildlife and Fisheries 12 2012-10-01 2012-10-01 false Content of a request for a financed program. 600.1003 Section 600.1003 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL... financed program shall: (a) Specify the reduction fishery. (b) Project the amount of the reduction and...

50 CFR 600.1003 - Content of a request for a financed program.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 10 2011-10-01 2011-10-01 false Content of a request for a financed program. 600.1003 Section 600.1003 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL... financed program shall: (a) Specify the reduction fishery. (b) Project the amount of the reduction and...

50 CFR 600.1003 - Content of a request for a financed program.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 50 Wildlife and Fisheries 12 2014-10-01 2014-10-01 false Content of a request for a financed program. 600.1003 Section 600.1003 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL... financed program shall: (a) Specify the reduction fishery. (b) Project the amount of the reduction and...

10 CFR 600.230 - Changes.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Changes. 600.230 Section 600.230 Energy DEPARTMENT OF... Changes. (a) General. Grantees and subgrantees are permitted to rebudget within the approved direct cost budget to meet unanticipated requirements and may make limited program changes to the approved project...

76 FR 8605 - Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 and 440...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-15

... after receipt. List of Subjects in 14 CFR Part 39 Air transportation, Aircraft, Aviation safety... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2010... Airworthiness Directives; Bombardier, Inc. Model CL-600-2B19 (Regional Jet Series 100 and 440) Airplanes AGENCY...

Gene encoding the group B streptococcal protein R4, its presence in clinical reference laboratory isolates & R4 protein pepsin sensitivity.

PubMed

Smith, B L; Flores, A; Dechaine, J; Krepela, J; Bergdall, A; Ferrieri, P

2004-05-01

R proteins were first identified by Lancefield in group B Streptococcus (GBS) as resistant to trypsin at pH8 and sensitive to pepsin at pH2. The R4 protein found predominantly in type III and some type II and V invasive isolates conforms to these criteria. The Rib protein, although structurally and epidemiologically similar to R4, was reported as resistant to both proteases. We report here the gene encoding the R4 protein from a type III group B streptococcal isolate (76-043) well characterized in our laboratory. Trypsin extracted GBS proteins were assayed for protease sensitivities by double-diffusion Ouchterlony using varying conditions for the enzyme pepsin. Standard haemoglobin assay was used to examine pepsin enzymatic activity. Thirty clinical isolates of varying protein profiles identified by double-diffusion from our reference strain laboratory were screened by PCR and Southern technique. SDS-PAGE gel purified R4 amino acid sequences were determined and used to design oligonucleotide primers for screening a 76-043 genomic library. R4 was sensitive to pepsin at pH2 but appeared resistant at pH4, the reported pH used for Rib. By standard haemoglobin assay and trypsin extract studies of R4 protein, pepsin was shown to be active at pH2, yet easily inactivated; assays of GBS surface proteins are critical at pH2. Of the amino acids initially sequenced from R4, 88 per cent (61/69) showed identity to Rib; the r4 nucleotide sequence was identical to that of rib. All isolates with strong positive protein reactions for R4 were positive in both PCR and Southern technique, whereas isolates expressing alpha, beta, R1/R4, and R5 (BPS) protein profiles were not. Sequenced PCR products aligned with identity to the R4 and Rib nucleotide sequences and confirmed the identity of these proteins and their molecular sequences.

10 CFR 600.233 - Supplies.

Code of Federal Regulations, 2010 CFR

2010-01-01

... supplies exceeding $5,000 in total aggregate fair market value upon termination or completion of the award... 10 Energy 4 2010-01-01 2010-01-01 false Supplies. 600.233 Section 600.233 Energy DEPARTMENT OF... Supplies. (a) Title. Title to supplies acquired under a grant or subgrant will vest, upon acquisition, in...

10 CFR 600.111 - Pre-award policies.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Requirements for Grants and Cooperative Agreements With Institutions of Higher Education, Hospitals, and Other Nonprofit Organizations Pre-Award Requirements § 600.111 Pre-award policies. (a) Use of Grants and... 10 Energy 4 2014-01-01 2014-01-01 false Pre-award policies. 600.111 Section 600.111 Energy...

10 CFR 600.20 - Maximum DOE obligation.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Maximum DOE obligation. 600.20 Section 600.20 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES General § 600.20 Maximum DOE obligation. (a) The maximum DOE obligation to the recipient is— (1) For monetary awards, the...

10 CFR 600.324 - Supplies.

Code of Federal Regulations, 2010 CFR

2010-01-01

... unused supplies. If the inventory of unused supplies exceeds $5,000 in total aggregate value and the... 10 Energy 4 2010-01-01 2010-01-01 false Supplies. 600.324 Section 600.324 Energy DEPARTMENT OF... Supplies. (a) Title vests in the recipient upon acquisition of supplies acquired with Federal funds under...

24 CFR 960.600 - Implementation.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Implementation. 960.600 Section 960.600 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR PUBLIC AND INDIAN HOUSING, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

10 CFR 600.137 - Property trust relationship.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Property trust relationship. 600.137 Section 600.137 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform..., Hospitals, and Other Nonprofit Organizations Post-Award Requirements § 600.137 Property trust relationship...

40 CFR 600.302-08 - Fuel economy label format requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... or diesel fuel as calculated in § 600.210-08(a) and (b). (3) The fuel pump logo. (4) The following... *”. The title shall be positioned in the grey area above the window of the fuel pump logo, in a size and...)”]”. Both of these titles are centered in the grey area above the window of the fuel pump logo, with a size...

40 CFR 600.302-08 - Fuel economy label format requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... or diesel fuel as calculated in § 600.210-08(a) and (b). (3) The fuel pump logo. (4) The following... *”. The title shall be positioned in the grey area above the window of the fuel pump logo, in a size and...)”]”. Both of these titles are centered in the grey area above the window of the fuel pump logo, with a size...

48 CFR 511.600 - Scope of subpart.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Scope of subpart. 511.600 Section 511.600 Federal Acquisition Regulations System GENERAL SERVICES ADMINISTRATION COMPETITION AND ACQUISITION PLANNING DESCRIBING AGENCY NEEDS Priorities and Allocations 511.600 Scope of subpart. Pursuant to...

48 CFR 511.600 - Scope of subpart.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Scope of subpart. 511.600 Section 511.600 Federal Acquisition Regulations System GENERAL SERVICES ADMINISTRATION COMPETITION AND ACQUISITION PLANNING DESCRIBING AGENCY NEEDS Priorities and Allocations 511.600 Scope of subpart. Pursuant to...

10 CFR 600.304 - Special award conditions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Special award conditions. 600.304 Section 600.304 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Administrative Requirements for Grants and Cooperative Agreements With For-Profit Organizations General § 600.304 Special...

15 CFR 971.600 - General.

Code of Federal Regulations, 2010 CFR

2010-01-01

... SERVICE DEEP SEABED MINING REGULATIONS FOR COMMERCIAL RECOVERY PERMITS Environmental Effects § 971.600... in a significant adverse environmental effect. In addition, each permit issued must contain TCRs... (section 109(b)), to prevent a significant adverse environmental effect. Furthermore, each permit issued...

77 FR 73343 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-10

...-1224; Directorate Identifier 2012-NM-112-AD] RIN 2120-AA64 Airworthiness Directives; Airbus Airplanes...: We propose to adopt a new airworthiness directive (AD) for all Airbus Model A300 B4-600, B4-600R, and... holidays. For Airbus service information identified in this proposed AD, contact Airbus SAS-EAW...

12 CFR 411.600 - Semi-annual compilation.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Semi-annual compilation. 411.600 Section 411.600 Banks and Banking EXPORT-IMPORT BANK OF THE UNITED STATES NEW RESTRICTIONS ON LOBBYING Agency Reports § 411.600 Semi-annual compilation. (a) The head of each agency shall collect and compile the...

50 CFR 600.910 - Definitions and word usage.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Definitions and word usage. 600.910..., Consultation, and Recommendations § 600.910 Definitions and word usage. (a) Definitions. In addition to the... undertaken by a state agency. (b) Word usage. The terms “must”, “shall”, “should”, “may”, “may not”, “will...

SH3BP4, a novel pigmentation gene, is inversely regulated by miR-125b and MITF

PubMed Central

Kim, Kyu-Han; Lee, Tae Ryong; Cho, Eun-Gyung

2017-01-01

Our previous work has identified miR-125b as a negative regulator of melanogenesis. However, the specific melanogenesis-related genes targeted by this miRNA had not been identified. In this study, we established a screening strategy involving three consecutive analytical approaches—analysis of target genes of miR-125b, expression correlation analysis between each target gene and representative pigmentary genes, and functional analysis of candidate genes related to melanogenesis—to discover melanogenesis-related genes targeted by miR-125b. Through these analyses, we identified SRC homology 3 domain-binding protein 4 (SH3BP4) as a novel pigmentation gene. In addition, by combining bioinformatics analysis and experimental validation, we demonstrated that SH3BP4 is a direct target of miR-125b. Finally, we found that SH3BP4 is transcriptionally regulated by microphthalmia-associated transcription factor as its direct target. These findings provide important insights into the roles of miRNAs and their targets in melanogenesis. PMID:28819321

Polarimetry of 600 pulsars from observations at 1.4 GHz with the Parkes radio telescope

NASA Astrophysics Data System (ADS)

Johnston, Simon; Kerr, Matthew

2018-03-01

Over the past 13 yr, the Parkes radio telescope has observed a large number of pulsars using digital filter bank backends with high time and frequency resolution and the capability for Stokes recording. Here, we use archival data to present polarimetry data at an observing frequency of 1.4 GHz for 600 pulsars with spin-periods ranging from 0.036 to 8.5 s. We comment briefly on some of the statistical implications from the data and highlight the differences between pulsars with high and low spin-down energy. The data set, images and table of properties for all 600 pulsars are made available in a public data archive maintained by the CSIRO.

Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells

PubMed Central

Yoon, Chan Jin; Middeldorp, Jaap M.; Martinez, Olivia M.; Byeon, Sun-ju; Rha, Sun Young; Kim, Sung Han; Kim, Yang Soo; Woo, Jun Hee

2016-01-01

Epstein-Barr virus (EBV)-encoded BamHI-A rightward frame 1 (BARF1) is a putative viral oncogene in EBV-infected stomach cancer. The aim of the present study was to investigate BARF1-induced cellular protein and microRNA alterations. In this study, BARF1-expressing stomach cancer cells showed a high rate of proliferation, high levels of NFκB, and miR-146a upregulation, which was reversed by NFκB knockdown. During BARF1-induced NFκB upregulation, hCSF1 receptor level was unchanged. Knockdown of BARF1 in the naturally EBV-infected YCCEL1 stomach cancer cells suppressed cell proliferation, and downregulated NFκB and miR-146a. SMAD4 was identified as a miR-146a target and was downregulated in BARF1-expressing cells, whereas SMAD4 expression was restored by anti-miR-146a. Knockdown of BARF1 in YCCEL1 cells upregulated SMAD4, and this effect was reversed by miR-146a overexpression. Transfection of BARF1-expressing cells with pCEP4-SMAD4 abolished the cell proliferating effect of BARF1. In stomach cancer tissues, miR-146a was expressed at higher levels, and more frequent NFκB nuclear positivity immunohistochemically, but not of SMAD4 nuclear loss was found in the EBV-positive group compared with the EBV-negative group. In conclusion, EBV-encoded BARF1 promotes cell proliferation in stomach cancer by upregulating NFκB and miR-146a and downregulating SMAD4, thereby contributing to EBV-induced stomach cancer progression. PMID:27438138

Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells.

PubMed

Kim, Dong Ha; Chang, Mee Soo; Yoon, Chan Jin; Middeldorp, Jaap M; Martinez, Olivia M; Byeon, Sun-Ju; Rha, Sun Young; Kim, Sung Han; Kim, Yang Soo; Woo, Jun Hee

2016-12-13

Epstein-Barr virus (EBV)-encoded BamHI-A rightward frame 1 (BARF1) is a putative viral oncogene in EBV-infected stomach cancer. The aim of the present study was to investigate BARF1-induced cellular protein and microRNA alterations. In this study, BARF1-expressing stomach cancer cells showed a high rate of proliferation, high levels of NFκB, and miR-146a upregulation, which was reversed by NFκB knockdown. During BARF1-induced NFκB upregulation, hCSF1 receptor level was unchanged. Knockdown of BARF1 in the naturally EBV-infected YCCEL1 stomach cancer cells suppressed cell proliferation, and downregulated NFκB and miR-146a. SMAD4 was identified as a miR-146a target and was downregulated in BARF1-expressing cells, whereas SMAD4 expression was restored by anti-miR-146a. Knockdown of BARF1 in YCCEL1 cells upregulated SMAD4, and this effect was reversed by miR-146a overexpression. Transfection of BARF1-expressing cells with pCEP4-SMAD4 abolished the cell proliferating effect of BARF1. In stomach cancer tissues, miR-146a was expressed at higher levels, and more frequent NFκB nuclear positivity immunohistochemically, but not of SMAD4 nuclear loss was found in the EBV-positive group compared with the EBV-negative group. In conclusion, EBV-encoded BARF1 promotes cell proliferation in stomach cancer by upregulating NFκB and miR-146a and downregulating SMAD4, thereby contributing to EBV-induced stomach cancer progression.

40 CFR 600.109-08 - EPA driving cycles.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 29 2010-07-01 2010-07-01 false EPA driving cycles. 600.109-08 Section... Model Year Automobiles-Test Procedures § 600.109-08 EPA driving cycles. (a) The FTP driving cycle is prescribed in § 86.115 of this chapter. (b) The highway fuel economy driving cycle is specified in this...

Comparison of 300,000 and 600,000 IU Oral Vitamin-D Bolus for Vitamin-D Deficiency in Young Children.

PubMed

Harnot, Jiyalal; Verma, Sanjay; Singhi, Sunit; Sankhyan, Naveen; Sachdeva, Naresh; Bharti, Bhavneet

2017-02-01

To compare the efficacy and safety of 300,000 and 600,000 IU vitamin-D single-oral dose for the treatment of vitamin-D deficiency (VDD) in young children (3 mo - 3 y). This double-blind randomized control trial (Clinical Trail Registration-CTRI/2012/05/002621) was conducted in the Pediatric out-patient department (OPD) at a tertiary-care referral hospital. Children (3 mo - 3 y) with clinical/radiological features suggestive of VDD were screened; those found to be having 25(OH)D below 15 ng/ml and meeting inclusion and exclusion criteria's were enrolled after taking informed consent. They were randomized into two groups, one receiving 600,000 and other 300,000 IU vitamin-D orally stat (Stoss-therapy). Primary outcome measure was proportion of children developing hypercalcemia/and hypercalciuria at day 7-10 post-therapy. Secondary outcome measures were proportion of children with hypercalciuria at day 3-5, hypercalcemia/and hypercalciuria at day 25-30 and 25(OH)D sufficiency at day 25-30 post-therapy. Sixty children, 30 in each group were randomized to two study groups. Baseline variables were comparable in two groups. Primary outcome measure (proportion of children with hypercalcemia/and hypercalciuria at 7 - 10th d) were 18.5 % (5/27) in 600,000 and 10.7 % (3/28) in 300,000 IU group (P = 0.47). Secondary outcome measures were - i) Proportion of children with hypercalciuria (3-5th d) were 18.5 % (5/27) in 600,000 and 7 % (2/28) in 300,000 group (P = 0.25). ii) Proportion of children with hypercalcemia/and hypercalciuria (25-30th d) were 18.5 % (5/27) in 600,000 and 11 % (3/28) in 300,000 group (P = 0.47). iii) All children in both groups had 25(OH)D levels in sufficiency range (25-30th d). With this sample size no significant difference in any of the group could be established. The superiority of 300,000 over 600,000 IU vitamin-D single-dose oral therapy for VDD in children (3 mo - 3 y) in terms of safety could not be established with this sample size

Complete genomic sequences for hepatitis C virus subtypes 4b, 4c, 4d, 4g, 4k, 4l, 4m, 4n, 4o, 4p, 4q, 4r and 4t.

PubMed

Li, Chunhua; Lu, Ling; Wu, Xianghong; Wang, Chuanxi; Bennett, Phil; Lu, Teng; Murphy, Donald

2009-08-01

In this study, we characterized the full-length genomic sequences of 13 distinct hepatitis C virus (HCV) genotype 4 isolates/subtypes: QC264/4b, QC381/4c, QC382/4d, QC193/4g, QC383/4k, QC274/4l, QC249/4m, QC97/4n, QC93/4o, QC139/4p, QC262/4q, QC384/4r and QC155/4t. These were amplified, using RT-PCR, from the sera of patients now residing in Canada, 11 of which were African immigrants. The resulting genomes varied between 9421 and 9475 nt in length and each contains a single ORF of 9018-9069 nt. The sequences showed nucleotide similarities of 77.3-84.3 % in comparison with subtypes 4a (GenBank accession no. Y11604) and 4f (EF589160) and 70.6-72.8 % in comparison with genotype 1 (M62321/1a, M58335/1b, D14853/1c, and 1?/AJ851228) reference sequences. These similarities were often higher than those currently defined by HCV classification criteria for subtype (75.0-80.0 %) and genotype (67.0-70.0 %) division, respectively. Further analyses of the complete and partial E1 and partial NS5B sequences confirmed these 13 'provisionally assigned subtypes'.

77 FR 59149 - Airworthiness Directives; Airbus Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-26

...-1002; Directorate Identifier 2012-NM-052-AD] RIN 2120-AA64 Airworthiness Directives; Airbus Airplanes...: We propose to adopt a new airworthiness directive (AD) for certain Airbus Model A300 B4-600, B4-600R...: Airbus: Docket No. FAA-2012-1002; Directorate Identifier 2012-NM- 052-AD. (a) Comments Due Date We must...

10 CFR 600.137 - Property trust relationship.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Property trust relationship. 600.137 Section 600.137..., Hospitals, and Other Nonprofit Organizations Post-Award Requirements § 600.137 Property trust relationship... Federal funds shall be held in trust by the recipient as trustee for the beneficiaries of the project or...

10 CFR 600.241 - Financial reporting.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Financial reporting. 600.241 Section 600.241 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform Administrative....241 Financial reporting. (a) General. (1) Except as provided in paragraphs (a) (2) and (5) of this...

10 CFR 600.241 - Financial reporting.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Financial reporting. 600.241 Section 600.241 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform Administrative....241 Financial reporting. (a) General. (1) Except as provided in paragraphs (a) (2) and (5) of this...

40 CFR 63.600 - Applicability.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Standards for Hazardous Air Pollutants From Phosphoric Acid Manufacturing Plants § 63.600 Applicability. (a... apply to the owner or operator of each phosphoric acid manufacturing plant. (b) The requirements of this... affected sources at a phosphoric acid manufacturing plant: (1) Each wet-process phosphoric acid process...

40 CFR 63.600 - Applicability.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Standards for Hazardous Air Pollutants From Phosphoric Acid Manufacturing Plants § 63.600 Applicability. (a... apply to the owner or operator of each phosphoric acid manufacturing plant. (b) The requirements of this... affected sources at a phosphoric acid manufacturing plant: (1) Each wet-process phosphoric acid process...

GATA4 promotes hepatoblastoma cell proliferation by altering expression of miR125b and DKK3.

PubMed

Pei, Yihua; Yao, Qin; Yuan, Sibo; Xie, Bozhen; Liu, Yan; Ye, Chunsheng; Zhuo, Huiqin

2016-11-22

GATA4 is a zinc finger DNA-binding protein that plays an important role in mammalian liver development. However, the effects of GATA4 in hepatoblastoma (HB), a common liver cancer in pediatric patients, remain largely unknown. In this study, we demonstrate that GATA4 promotes growth and survival in the Huh6 human hepatoblastoma cell line. GATA4 expression was high in Huh6 cells, and its knockdown decreased expression of Dickkopf-related protein 3 (DKK3), a gene that may contribute to premature or undifferentiated phenotypes in HB. GATA4 also directly bound to the promoter regions of the miRNA miR125b and inhibited its expression in Huh6 cells. DKK3 was a direct target of miR125b in Huh6 cells. Inhibition of miR125b or overexpression of DKK3 promoted proliferation, survival, migration, and invasion in Huh6 cells. This is the first report to demonstrate that GATA4 promotes oncogenesis by inhibiting miR125b-dependent suppression of DKK3 expression. This GATA4/miR125b/DKK3 axis may be a major regulator of growth, migration, invasion, and survival in hepatoma cells, and is therefore a potential therapeutic target or biomarker for progression in HB patients.

24 CFR 954.600 - Performance reviews.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Performance reviews. 954.600... DEVELOPMENT INDIAN HOME PROGRAM Performance Reviews and Sanctions § 954.600 Performance reviews. (a) General. HUD will review the performance of each grantee in carrying out its responsibilities under this part...

24 CFR 954.600 - Performance reviews.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 4 2011-04-01 2011-04-01 false Performance reviews. 954.600... DEVELOPMENT INDIAN HOME PROGRAM Performance Reviews and Sanctions § 954.600 Performance reviews. (a) General. HUD will review the performance of each grantee in carrying out its responsibilities under this part...

40 CFR 600.107-08 - Fuel specifications.

Code of Federal Regulations, 2014 CFR

2014-07-01

... Exhaust Emission Test Procedures § 600.107-08 Fuel specifications. (a) The test fuel specifications for... given in paragraph (b) of this section. (b)(1) Diesel test fuel used for cold temperature FTP testing... alternative fuel for cold temperature FTP testing. (c) Test fuels representing fuel types for which there are...

40 CFR 600.107-08 - Fuel specifications.

Code of Federal Regulations, 2013 CFR

2013-07-01

... Exhaust Emission Test Procedures § 600.107-08 Fuel specifications. (a) The test fuel specifications for... given in paragraph (b) of this section. (b)(1) Diesel test fuel used for cold temperature FTP testing... alternative fuel for cold temperature FTP testing. (c) Test fuels representing fuel types for which there are...

40 CFR 600.107-08 - Fuel specifications.

Code of Federal Regulations, 2012 CFR

2012-07-01

... Exhaust Emission Test Procedures § 600.107-08 Fuel specifications. (a) The test fuel specifications for... given in paragraph (b) of this section. (b)(1) Diesel test fuel used for cold temperature FTP testing... alternative fuel for cold temperature FTP testing. (c) Test fuels representing fuel types for which there are...

10 CFR 600.225 - Program income.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Program income. 600.225 Section 600.225 Energy DEPARTMENT....225 Program income. (a) General. Grantees are encouraged to earn income to defray program costs. Program income includes income from fees for services performed, from the use or rental of real or...

10 CFR 600.231 - Real property.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Real property. 600.231 Section 600.231 Energy DEPARTMENT....231 Real property. (a) Title. Subject to the obligations and conditions set forth in this section, title to real property acquired under a grant or subgrant will vest upon acquisition in the grantee or...

10 CFR 600.231 - Real property.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Real property. 600.231 Section 600.231 Energy DEPARTMENT....231 Real property. (a) Title. Subject to the obligations and conditions set forth in this section, title to real property acquired under a grant or subgrant will vest upon acquisition in the grantee or...

10 CFR 600.231 - Real property.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Real property. 600.231 Section 600.231 Energy DEPARTMENT....231 Real property. (a) Title. Subject to the obligations and conditions set forth in this section, title to real property acquired under a grant or subgrant will vest upon acquisition in the grantee or...

10 CFR 600.231 - Real property.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Real property. 600.231 Section 600.231 Energy DEPARTMENT....231 Real property. (a) Title. Subject to the obligations and conditions set forth in this section, title to real property acquired under a grant or subgrant will vest upon acquisition in the grantee or...

10 CFR 600.231 - Real property.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Real property. 600.231 Section 600.231 Energy DEPARTMENT....231 Real property. (a) Title. Subject to the obligations and conditions set forth in this section, title to real property acquired under a grant or subgrant will vest upon acquisition in the grantee or...

Prognostic significance of B7-H4 expression in matched primary pancreatic cancer and liver metastases.

PubMed

Qian, Yun; Sang, Yiwen; Wang, Frederick X C; Hong, Bo; Wang, Qi; Zhou, Xinhui; Weng, Tianhao; Wu, Zhigang; Zheng, Min; Zhang, Hong; Yao, Hangping

2016-11-01

Liver metastasis development in pancreatic cancer patients is common and confers a poor prognosis. Clinical relevance of biomarker analysis in metastatic tissue is necessary. B7-H4 has an inhibitory effect on T cell mediated response and may be involved in tumor development. Although B7-H4 expression has been detected in pancreatic cancer, its expression in liver metastases from pancreatic cancer is still unknown. In this study, overall 43 pancreatic cancer liver metastases (with matched primaries in 15/43 cases) and 57 pancreatic cancer cases without liver metastases or other distant metastases were analyzed for their expression of B7-H4 by immunohistochemistry. Survival curves and log-rank tests were used to test the association of B7-H4 expression with survival. B7-H4 was highly expressed in 28 (65.1%) of the 43 liver metastases and 9 (60.0%) of the 15 matched primary tumors. The expression of B7-H4 in liver metastases was significantly higher than in the matched primary tumors (p < 0.05). Patients with high B7-H4 expression in their primary pancreatic cancer had higher risk of developing liver metastases (p < 0.05). In univariate analysis, B7-H4 expression was significantly associated with the risk of death (p < 0.05). And the multivariate analysis identified that B7-H4 was an independent prognostic indicator (p < 0.05). Our results revealed B7-H4 to be associated with poor prognosis in patients with pancreatic cancer liver metastasis. B7-H4 may promote pancreatic cancer metastasis and was promising to be a potential prognostic indicator of pancreatic cancer.

50 CFR 600.355 - National Standard 10-Safety of Life at Sea.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Sea. 600.355 Section 600.355 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL... Standards § 600.355 National Standard 10—Safety of Life at Sea. (a) Standard 10. Conservation and management measures shall, to the extent practicable, promote the safety of human life at sea. (b) General. (1...

10 CFR 600.502 - What must DOE determine.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false What must DOE determine. 600.502 Section 600.502 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Eligibility Determination for Certain Financial Assistance Programs-General Statement of Policy § 600.502 What must DOE...

Using new non-invasive quick method to detect Borrelia Burgdorferi (B.B.) infection from specific parts of the heart in "seemingly normal" ECGs, and from the ECGs of Atrial Fibrillation (AF), a majority of AF ECGs are found to have: 1) Significant B.B. infection, 2) Markedly increased ANP, 3) Increased Cardiac Troponin I & 4) Markedly reduced Taurine. These 4 factors were mainly localized at infected areas of the SA node area, R-&L-Atria & pulmonary veins at the L-atrium.

PubMed

Omura, Yoshiaki; Lu, Dominic; Jones, Marilyn K; Nihrane, Abdallah; Duvvi, Harsha; Yapor, Dario; Shimotsuura, Yasuhiro; Ohki, Motomu

2015-01-01

Lyme disease is found in a majority of people we tested. Once Borrelia Burgdorferi (B.B.) spirochete enters human body, it not only causes pain by infecting joints, but it also often enters the brain and the heart. Infection of brain can be quickly detected from the pupil and infection of the heart by ECGs non-invasively. By evaluating recorded ECGs of atrial fibrillation (AF), using U.S. patented non-invasive highly sensitive electromagnetic field (EMF) resonance phenomenon between 2 identical molecules or between a molecule and its antibody, we examined 25 different AF patients' ECGs and found the majority of them suffer from various degrees of B.B. spirochete infection in SA node areas, also in the right & left atria, and pulmonary vein near and around its junction at left atrium & lesser degrees of infection at the AV node & His Bundle. When B.B. infection reaches over 224-600ng or higher at these areas, AF often appears in the majority of all AF analyzed. In order to develop AF, the 4 abnormal factors must be present simultaneously: 1) B.B. infection must be increased to 224-600ng or higher, 2) Atrial Natriuretic Peptide (ANP) must be markedly reduced from normal value of less than 4ng to over 100-400ng, 3) A significant increase of Cardiac Troponin I from normal value of less than 3ng to over 12ng and 4) Taurine must also be markedly reduced from normal value of 4-6ng to 0.25ng. These 4 changes were mainly found only at infected sites of the SA node area, both atria and between the end of the T wave & the beginning of the SA node area, which corresponds to U waves at recorded ECG. Origin of the U wave is mainly due to abnormal electrical potential of pulmonary vein at L-atrium. If all 4 factors do not occur at the infection site, no AF will develop. In seemingly normal ECGs, if using this method, one can detect invisible B.B. infection in early stages. Long before AF appears, AF can be prevented by improved treatment with Amoxicillin 500ng 3 times

76 FR 15870 - Airworthiness Directives; Airbus Model A310 Series Airplanes, and Airbus Model A300 B4-600, B4...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-22

..., Docket Operations, M-30, West Building Ground Floor, Room W12-140, 1200 New Jersey Avenue, SE... Building Ground Floor, Room W12-40, 1200 New Jersey Avenue, SE., Washington, DC, between 9 a.m. and 5 p.m... than estimated here. Based on these figures, we estimate the cost of the proposed AD on U.S. operators...

10 CFR 600.122 - Payment.

Code of Federal Regulations, 2010 CFR

2010-01-01

... codified at 31 CFR part 205. (b) Recipients will be paid in advance, provided they maintain or demonstrate... standards for fund control and accountability as established in § 600.121. Cash advances to a recipient... program or project. The timing and amount of cash advances shall be as close as is administratively...

75 FR 8461 - Airworthiness Directives; Bombardier, Inc. Model CL-600-1A11 (CL-600), CL-600-2A12 (CL-601), and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-25

... Model CL-600-1A11 AFM (Winglets). These two TRs are approved by TCCA, and should be replaced in AD 2009... the Canadair Challenger Model CL-600-1A11 AFM (Winglets). We agreed and proposed to revise Table 2 of...) 600-1/17 August 16, 2006.. Canadair Challenger Model CL-600-1A11 AFM (Winglets). (iii) 601/14 August...

10 CFR 600.145 - Cost and price analysis.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Cost and price analysis. 600.145 Section 600.145 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform Administrative... Nonprofit Organizations Post-Award Requirements § 600.145 Cost and price analysis. Some form of cost or...

10 CFR 600.102 - Effect on other issuances.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Effect on other issuances. 600.102 Section 600.102 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform Administrative... Nonprofit Organizations General § 600.102 Effect on other issuances. For awards subject to this subpart, all...

10 CFR 600.102 - Effect on other issuances.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Effect on other issuances. 600.102 Section 600.102 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform Administrative... Nonprofit Organizations General § 600.102 Effect on other issuances. For awards subject to this subpart, all...

10 CFR 600.204 - Effect on other issuances.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Effect on other issuances. 600.204 Section 600.204 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform Administrative Requirements for Grants and Cooperative Agreements to State and Local Governments General § 600.204 Effect on...

High radio fluxes of PKS2023-07 measured with RATAN-600

NASA Astrophysics Data System (ADS)

Trushkin, S. A.; Nizhelskij, N. A.; Tsybulev, P. G.

2016-04-01

After the AGILE detection the gamma-ray flare from the quasar PSK2023-07 (correctly PKS B2022-077) by Piano et al, ATel #8879 we carried out its observations with the RATAN-600 radio telescope SAO RAS. The measured flux densities are equal to 1.50, 1.96, 2.54 Jy at 4.8, 11.2, 21.7 GHz respectively on 1 Apr 2016.

10 CFR 600.8 - Funding Opportunity Announcement.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Funding Opportunity Announcement. 600.8 Section 600.8... Funding Opportunity Announcement. (a) General. Funding Opportunity Announcements (FOA) include any issuance used to announce funding opportunities that would result in the award of a discretionary grant...

10 CFR 600.8 - Funding Opportunity Announcement.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Funding Opportunity Announcement. 600.8 Section 600.8... Funding Opportunity Announcement. (a) General. Funding Opportunity Announcements (FOA) include any issuance used to announce funding opportunities that would result in the award of a discretionary grant...

Mercury-Bridged Cobaltacarborane Complexes Containing B-Hg-B Three-Center Bonds. Synthesis and Structure of mu, mu’-((n5-C5R5)Co(CH3)2C2B3H4)Hg, mu-(n(5)-C5R5)Co(CH3)2C2B3H4)HgCl, (R=H, CH3) and Related Compounds.

DTIC Science & Technology

1980-11-01

MERCURY-BRIDGED COBALTACARBORANE COMPLEXES CONTAINING B-HG-B TH--ETC(U) NOV 80 D C FINSTER . R N GRIMES N0 0 0 1 4-75-0305 UNCLASSXFIED TR󈧨 NL ILn...C5R5) Co 3)2C2B3 4 2 5 .- -C5R5 )Co(CH3)2C2B3H4 ]HgCl, (R=H, CH3 ) and Related Compounds, David C./ Finster -- Russell N./Grimes ( Department of Chemistry...Compounds 1 \\David C. Finster And Russell N. Grimes* Abstract. Reactions of the nid~p-cobaltacarborane anions 01CR )(C 3 )C BH and [n (H 1oC ihH~5n 5

Dibenzo[b,f][1,4]oxazepines and dibenzo[b,e]oxepines: Influence of the chlorine substitution pattern on the pharmacology at the H1R, H4R, 5-HT2AR and other selected GPCRs.

PubMed

Naporra, Franziska; Gobleder, Susanne; Wittmann, Hans-Joachim; Spindler, Julia; Bodensteiner, Michael; Bernhardt, Günther; Hübner, Harald; Gmeiner, Peter; Elz, Sigurd; Strasser, Andrea

2016-11-01

Inspired by VUF6884 (7-Chloro-11-(4-methylpiperazin-1-yl)dibenzo[b,f][1,4]oxazepine), reported as a dual H 1 /H 4 receptor ligand (pK i : 8.11 (human H 1 R (hH 1 R)), 7.55 (human H 4 R (hH 4 R))), four known and 28 new oxazepine and related oxepine derivatives were synthesised and pharmacologically characterized at histamine receptors and selected aminergic GPCRs. In contrast to the oxazepine series, within the oxepine series, the new compounds showed high affinity to the hH 1 R (pK i : 6.8-8.7), but no or moderate affinity to the hH 4 R (pK i :≤5.3). For one oxepine derivative (1-(2-Chloro-6,11-dihydrodibenzo[b,e]oxepin-11-yl)-4-methylpiperazine), the enantiomers were separated and the R-enantiomer was identified as the eutomer at the hH 1 R (pK i : 8.83 (R), 7.63 (S)) and the guinea-pig H 1 R (gpH 1 R) (pK i : 8.82 (R), 7.41 (S)). Molecular dynamic studies suggest that the tricyclic core of the compounds is bound in a similar mode into the binding pocket, as described for doxepine in the hH 1 R crystal structure. Moreover, docking studies of all oxepine derivatives at the hH 1 R indicate that the oxygen and the position of the chlorine in the tricyclic core determines, if the R- or the S-enantiomer is the eutomer. For some of the oxazepines and oxepines the affinity to other aminergic GPCRs is in the same range as to hH 1 R or hH 4 R, thus, those compounds have to be classified as dirty drugs. However, one oxazepine derivative (3,7-Dichloro-11-(4-methylpiperazin-1-yl)dibenzo[b,f][1,4]oxazepine was identified as dual hH 1 /h5-HT 2A receptor ligand (pK i : 9.23 (hH 1 R), 8.74 (h5-HT 2A R), ≤7 at other analysed GPCRs), whereas one oxepine derivative (1-(3,8-Dichloro-6,11-dihydrodibenzo[b,e]oxepin-11-yl)-4-methylpiperazine) was identified as selective hH 1 R antagonist (pK i : 8.44 (hH 1 R), ≤6.7 at other analyzed GPCRs). Thus, the pharmacological results suggest that the oxazepine/oxepine moiety and additionally the chlorine substitution pattern toggles

10 CFR 600.8 - Funding Opportunity Announcement.

Code of Federal Regulations, 2010 CFR

2010-01-01

... of eligible applicants live outside of the United States. (ii) Single source announcements of funding... 10 Energy 4 2010-01-01 2010-01-01 false Funding Opportunity Announcement. 600.8 Section 600.8... Funding Opportunity Announcement. (a) General. Funding Opportunity Announcements (FOA) include any...

10 CFR 600.115 - Metric system of measurement.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Metric system of measurement. 600.115 Section 600.115..., Hospitals, and Other Nonprofit Organizations Pre-Award Requirements § 600.115 Metric system of measurement...) declares that the metric system is the preferred measurement system for U.S. trade and commerce. The Act...

10 CFR 600.115 - Metric system of measurement.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Metric system of measurement. 600.115 Section 600.115..., Hospitals, and Other Nonprofit Organizations Pre-Award Requirements § 600.115 Metric system of measurement...) declares that the metric system is the preferred measurement system for U.S. trade and commerce. The Act...

10 CFR 600.115 - Metric system of measurement.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Metric system of measurement. 600.115 Section 600.115..., Hospitals, and Other Nonprofit Organizations Pre-Award Requirements § 600.115 Metric system of measurement...) declares that the metric system is the preferred measurement system for U.S. trade and commerce. The Act...

10 CFR 600.115 - Metric system of measurement.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Metric system of measurement. 600.115 Section 600.115..., Hospitals, and Other Nonprofit Organizations Pre-Award Requirements § 600.115 Metric system of measurement...) declares that the metric system is the preferred measurement system for U.S. trade and commerce. The Act...

10 CFR 600.115 - Metric system of measurement.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Metric system of measurement. 600.115 Section 600.115..., Hospitals, and Other Nonprofit Organizations Pre-Award Requirements § 600.115 Metric system of measurement...) declares that the metric system is the preferred measurement system for U.S. trade and commerce. The Act...

10 CFR 600.171 - Closeout procedures.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Nonprofit Organizations After-The-Award Requirements § 600.171 Closeout procedures. (a) Recipients shall..., and other reports as required by the terms and conditions of the award. DOE may approve extensions when requested by the recipient. (b) Unless DOE authorizes an extension, a recipient shall liquidate...

10 CFR 600.171 - Closeout procedures.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Nonprofit Organizations After-The-Award Requirements § 600.171 Closeout procedures. (a) Recipients shall..., and other reports as required by the terms and conditions of the award. DOE may approve extensions when requested by the recipient. (b) Unless DOE authorizes an extension, a recipient shall liquidate...

10 CFR 600.171 - Closeout procedures.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Nonprofit Organizations After-The-Award Requirements § 600.171 Closeout procedures. (a) Recipients shall..., and other reports as required by the terms and conditions of the award. DOE may approve extensions when requested by the recipient. (b) Unless DOE authorizes an extension, a recipient shall liquidate...

10 CFR 600.171 - Closeout procedures.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Nonprofit Organizations After-The-Award Requirements § 600.171 Closeout procedures. (a) Recipients shall..., and other reports as required by the terms and conditions of the award. DOE may approve extensions when requested by the recipient. (b) Unless DOE authorizes an extension, a recipient shall liquidate...

10 CFR 600.171 - Closeout procedures.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Nonprofit Organizations After-The-Award Requirements § 600.171 Closeout procedures. (a) Recipients shall..., and other reports as required by the terms and conditions of the award. DOE may approve extensions when requested by the recipient. (b) Unless DOE authorizes an extension, a recipient shall liquidate...

76 FR 15805 - Airworthiness Directives; Airbus Model A310 Series Airplanes, and Airbus Model A300 B4-600, B4...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-22

... greater than 100 knots and until landing gear retraction. * * * * * Uncommanded movement of the pilot and... until landing gear retraction. AD 2009-0084 required the deactivation of the electrical power of SOGERMA... takeoff phase when the speed of the aeroplane is greater than 100 knots and until landing gear retraction...

Enhanced extracellular production of L-asparaginase from Bacillus subtilis 168 by B. subtilis WB600 through a combined strategy.

PubMed

Feng, Yue; Liu, Song; Jiao, Yun; Gao, Hui; Wang, Miao; Du, Guocheng; Chen, Jian

2017-02-01

L-asparaginase (EC 3.5.1.1, ASN) exhibits great commercial value due to its uses in the food and medicine industry. In this study, we reported the enhanced expression of type II ASN from Bacillus subtilis 168 in B. subtilis WB600 through a combined strategy. First, eight signal peptides (the signal peptide of the ASN, ywbN, yvgO, amyE, oppA, vpr, lipA, and wapA) were used for ASN secretion in B. subtilis by using Hpa II promoter, respectively. The signal peptide wapA achieved the highest extracellular ASN activity (28.91 U/mL). Second, Hpa II promoter was replaced by a strong promoter, P43 promoter, resulting in 38.1 % enhanced ASN activity. By two rounds of error-prone PCR mutation, the P43 promoter variants with remarkably enhanced strength (D7, E2, H6, B2, and F3) were identified. B2 (-28: A → G, -13: A → G) achieved ASN activity up to 51.13 U/mL. Third, after deletion of the N-terminal 25-residues, ASN activity reached 102.41 U/mL, which was 100 % higher than that of the intact ASN. At last, the extracellular ASN of the B. subtilis arrived at 407.6 U/mL (2.5 g/L of ASN protein) in a 3-L bioreactor by using a fed-batch strategy. The purified ASN showed maximal activity at 65 °C and its half-life at 65 °C was 61 min. The K m and k cat of the ASN were 5.29 mM and 54.4 s -1 , respectively. To the best of our knowledge, we obtained the highest yield of ASN in a food-grade host ever reported, which may benefit the industrial production and application of ASN.

B7-H4 as a Target for Breast Cancer Immunotherapy

DTIC Science & Technology

2012-06-01

lymphoma and leukemia cell lines. CEM, Karpas 299, and TLBR -1, cell lines derived from acute T-cell lymphoblastic leukemia, large cell anaplastic...Accomplishments  Generation of human B7-H4-Fc fusion protein (antigen).  Discovery of a B7-H4 receptor on CEM, Karpas 299, and TLBR -1 cell lines...CEM Karpas 299 TLBR -1 Jurkat B7-H4R Figure 3. B7-H4 binding to human T-cell lymphoma cell lines. Red

40 CFR 600.310-86 - Labeling of high altitude vehicles.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 30 2011-07-01 2011-07-01 false Labeling of high altitude vehicles... Regulations for 1977 and Later Model Year Automobiles-Labeling § 600.310-86 Labeling of high altitude vehicles... altitude vehicles according to § 600.306. (b) A high altitude vehicle may be labeled with a general or...

40 CFR 600.310-86 - Labeling of high altitude vehicles.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Labeling of high altitude vehicles... Regulations for 1977 and Later Model Year Automobiles-Labeling § 600.310-86 Labeling of high altitude vehicles... altitude vehicles according to § 600.306. (b) A high altitude vehicle may be labeled with a general or...

10 CFR 600.10 - Form and content of applications.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Form and content of applications. 600.10 Section 600.10 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES General § 600.10 Form and content of applications. (a) General. Applications shall be required for all financial...

10 CFR 600.10 - Form and content of applications.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Form and content of applications. 600.10 Section 600.10 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES General § 600.10 Form and content of applications. (a) General. Applications shall be required for all financial...

10 CFR 600.120 - Purpose of financial and program management.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Purpose of financial and program management. 600.120 Section 600.120 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES... financial and program management. Sections 600.121 through 600.128 prescribe standards for financial...

10 CFR 600.323 - Property management system.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Property management system. 600.323 Section 600.323 Energy....323 Property management system. The recipient's property management system must include the following... existence, current utilization, and continued need for the property. (d) A control system must be in effect...

10 CFR 600.323 - Property management system.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Property management system. 600.323 Section 600.323 Energy....323 Property management system. The recipient's property management system must include the following... existence, current utilization, and continued need for the property. (d) A control system must be in effect...

40 CFR 600.306-08 - Labeling requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... which has a combined FTP/HFET-based fuel economy value, as determined in § 600.513-08, at or below the...) Have not been delivered to the ultimate purchaser, and (B) Have a combined FTP/HFET-based model type...

Catalytic properties of thimet oligopeptidase H600A mutant

DOE Office of Scientific and Technical Information (OSTI.GOV)

Machado, Mauricio F.M.; Marcondes, Marcelo F.; Rioli, Vanessa

2010-04-02

Thimet oligopeptidase (EC 3.4.24.15, TOP) is a metallo-oligopeptidase that participates in the intracellular metabolism of peptides. Predictions based on structurally analogous peptidases (Dcp and ACE-2) show that TOP can present a hinge-bend movement during substrate hydrolysis, what brings some residues closer to the substrate. One of these residues that in TOP crystallographic structure are far from the catalytic residues, but, moves toward the substrate considering this possible structural reorganization is His{sup 600}. In the present work, the role of His{sup 600} of TOP was investigated by site-directed mutagenesis. TOP H600A mutant was characterized through analysis of S{sub 1} and S{submore » 1}' specificity, pH-activity profile and inhibition by JA-2. Results showed that TOP His{sup 600} residue makes important interactions with the substrate, supporting the prediction that His{sup 600} moves toward the substrate due to a hinge movement similar to the Dcp and ACE-2. Furthermore, the mutation H600A affected both K{sub m} and k{sub cat}, showing the importance of His{sup 600} for both substrate binding and/or product release from active site. Changes in the pH-profile may indicate also the participation of His{sup 600} in TOP catalysis, transferring a proton to the newly generated NH{sub 2}-terminus or helping Tyr{sup 605} and/or Tyr{sup 612} in the intermediate oxyanion stabilization.« less

10 CFR 600.310 - Purpose of financial and program management.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Purpose of financial and program management. 600.310... Requirements § 600.310 Purpose of financial and program management. Sections 600.311 through 600.318 prescribe standards for financial management systems; methods for making payments; and rules for cost sharing and...

50 CFR 600.1004 - Accepting a request for, and determinations about initiating, a financed program.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Accepting a request for, and... meets, or will meet after an appropriate reduction amendment, the requirements in § 600.1002(a); (2) The CFMP meets, or will meet after an appropriate reduction amendment, the requirements in § 600.1002(b...

10 CFR 600.17 - Contents of Award.

Code of Federal Regulations, 2013 CFR

2013-01-01

... of DOE and the recipient, consistent with the requirements of this part. [74 FR 44276, Aug. 28, 2009] ... 10 Energy 4 2013-01-01 2013-01-01 false Contents of Award. 600.17 Section 600.17 Energy DEPARTMENT... Award. Each financial assistance award shall be made on a Notice of Financial Assistance Award (NFAA...

10 CFR 600.17 - Contents of Award.

Code of Federal Regulations, 2012 CFR

2012-01-01

... of DOE and the recipient, consistent with the requirements of this part. [74 FR 44276, Aug. 28, 2009] ... 10 Energy 4 2012-01-01 2012-01-01 false Contents of Award. 600.17 Section 600.17 Energy DEPARTMENT... Award. Each financial assistance award shall be made on a Notice of Financial Assistance Award (NFAA...

10 CFR 600.17 - Contents of Award.

Code of Federal Regulations, 2014 CFR

2014-01-01

... of DOE and the recipient, consistent with the requirements of this part. [74 FR 44276, Aug. 28, 2009] ... 10 Energy 4 2014-01-01 2014-01-01 false Contents of Award. 600.17 Section 600.17 Energy DEPARTMENT... Award. Each financial assistance award shall be made on a Notice of Financial Assistance Award (NFAA...

10 CFR 600.17 - Contents of Award.

Code of Federal Regulations, 2011 CFR

2011-01-01

... of DOE and the recipient, consistent with the requirements of this part. [74 FR 44276, Aug. 28, 2009] ... 10 Energy 4 2011-01-01 2011-01-01 false Contents of Award. 600.17 Section 600.17 Energy DEPARTMENT... Award. Each financial assistance award shall be made on a Notice of Financial Assistance Award (NFAA...

10 CFR 600.17 - Contents of Award.

Code of Federal Regulations, 2010 CFR

2010-01-01

... of DOE and the recipient, consistent with the requirements of this part. [74 FR 44276, Aug. 28, 2009] ... 10 Energy 4 2010-01-01 2010-01-01 false Contents of Award. 600.17 Section 600.17 Energy DEPARTMENT... Award. Each financial assistance award shall be made on a Notice of Financial Assistance Award (NFAA...

10 CFR 600.126 - Non-Federal audits.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Non-Federal audits. 600.126 Section 600.126 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Uniform Administrative Requirements for Grants and Cooperative Agreements With Institutions of Higher Education, Hospitals, and Other Nonprofit Organizations Post-Award...

50 CFR 600.1006 - Accepting a request for, and determinations about conducting, a subsidized program.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Accepting a request for, and... reduction amendment, the requirements in § 600.1002(a); (2) Certify that the CFMP meets, or will meet after an appropriate reduction amendment, the requirements in § 600.1002(b)(1) and (2); and (3) Project the...

Usefulness of immunohistochemistry for the detection of the BRAF V600E mutation in Japanese lung adenocarcinoma.

PubMed

Sasaki, Hidefumi; Shimizu, Shigeki; Tani, Yoichi; Shitara, Masayuki; Okuda, Katsuhiro; Hikosaka, Yu; Moriyama, Satoru; Yano, Motoki; Fujii, Yoshitaka

2013-10-01

Mutations in components of the mitogen-activated protein kinase (MAPK) cascade may be a new candidate for target for lung cancer. The usefulness of immunohistochemistry (IHC) as a new approach for the detection of BRAF V600E in cancer patients has been recently reported. To increase the sensitivity, we modified BRAF V600E expression detection assay by IHC using mutation specific antibody. From the screening step, we found a novel 599 insertion T BRAF mutation in lung adenocarcinoma. In this study included 26 surgically removed cases with EGFR, Kras, erbB2, EML4-ALK and KIF5B-RET wild-type (wt) lung adenocarcinomas, including 7 BRAF mutants (5 V600E, 1 N581I, and 1 novel 599 insertion T mutation) analyzed by DNA sequencing. Detection of the BRAF V600E mutation was carried out by the Dako EnVision™ FLEX detection system using the VE1 clone antibody and compared with the results of direct sequencing. The autostainer IHC VE1 assay was positive in 5 of 5 (100%) BRAF V600E-mutated tumors and negative in 20 of 21 (95.2%) BRAF non-V600E tumors, except for a novel 599 insertion T case. IHC using the VE1 clone and FLEX linker is a specific method for the detection BRAF V600E and may be an alternative to molecular biology for the detection of mutations in lung adenocarcinomas. This method might be useful for screening to use molecular target therapy for lung adenocarcinomas. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

17 CFR Appendix B to Part 190 - Special Bankruptcy Distributions

Code of Federal Regulations, 2012 CFR

2012-04-01

.... Customer Claim Location(s) customer has consented to having funds held A $50 U.S. B €50 U.K. C €50 Germany D £300 U.K. Location Actual asset balance U.S. $50 U.K. £300 U.K. €50 Germany €50 Note: Conversion... Germany €50 1.0 50 50 Total 600.00 0 600.00 There are no shortfalls in funds held in any location...

300,000 IU or 600,000 IU of oral vitamin D3 for treatment of nutritional rickets: a randomized controlled trial.

PubMed

Mittal, Hema; Rai, Sunita; Shah, Dheeraj; Madhu, S V; Mehrotra, Gopesh; Malhotra, Rajeev Kumar; Gupta, Piyush

2014-04-01

To evaluate the non-inferiority of a lower therapeutic dose (300,000 IU) in comparison to standard dose (600,000) IU of Vitamin D for increasing serum 25(OH) D levels and achieving radiological recovery in nutritional rickets. Randomized, open-labeled, controlled trial. Tertiary care hospital. 76 children (median age 12 mo) with clinical and radiologically confirmed rickets. Oral vitamin D3 as 300,000 IU (Group 1; n=38) or 600,000 IU (Group 2; n=38) in a single day. Primary: Serum 25(OH)D, 12 weeks after administration of vitamin D3; Secondary: Radiological healing and serum parathormone at 12 weeks; and clinical and biochemical adverse effects. Serum 25(OH)D levels [geometric mean (95% CI)] increased significantly from baseline to 12 weeks after therapy in both the groups [Group 1: 7.58 (5.50–10.44) to 16.06 (12.71– 20.29) ng/mL, P<0.001]; Group 2: 6.57 (4.66–9.25) to 17.60 (13.71–22.60, P<0.001]. The adjusted ratio of geometric mean serum 25(OH)D levels at 12 weeks between the groups (taking baseline value as co-variate) was 0.91 (95% CI: 0.65–1.29). Radiological healing occurred in all children by 12 weeks. Both groups demonstrated significant (P<0.05) and comparable fall in the serum parathormone and alkaline phosphatase levels at 12 weeks. Relative change [ratio of geometric mean (95% CI)] in serum PTH and alkaline phosphatase, 12 weeks after therapy, were 0.98 (0.7–1.47) and 0.92 (0.72–1.19), respectively. The serum 25(OH)D levels were deficient (<20 ng/mL) in 63% (38/60) children after 12 weeks of intervention [Group 1: 20/32 (62.5%); Group 2: 18/28 (64.3%)]. No major clinical adverse effects were noticed in any of the children. Hypercalcemia was documented in 2 children at 4 weeks (1 in each Group) and 3 children at 12 weeks (1 in Group 1 and 2 in Group 2). None of the participants had hypercalciuria or hypervitaminosis D. A dose of 300,000 IU of vitamin D3 is comparable to 600,000 IU, administered orally, over a single day, for treating

Seizures induced in immature rats by homocysteic acid and the associated brain damage are prevented by group II metabotropic glutamate receptor agonist (2R,4R)-4-aminopyrrolidine-2,4-dicarboxylate.

PubMed

Folbergrová, Jaroslava; Druga, Rastislav; Otáhal, Jakub; Haugvicová, Renata; Mares, Pavel; Kubová, Hana

2005-04-01

The present study has examined the anticonvulsant and neuroprotective effect of group II metabotropic glutamate receptor (mGluR) agonist (2R,4R)-4-aminopyrrolidine-2,4-dicarboxylate (2R,4R-APDC) in the model of seizures induced in immature 12-day-old rats by bilateral intracerebroventricular infusion of dl-homocysteic acid (DL-HCA, 600 nmol/side). For biochemical analyses, rat pups were sacrificed during generalized clonic-tonic seizures, approximately 45-50 min after infusion. Comparable time intervals were used for sacrificing the pups which had received 2R,4R-APDC. Low doses of 2R,4R-APDC (0.05 nmol/side) provided a pronounced anticonvulsant effect which was abolished by pretreatment with a selective group II mGluR antagonist LY341495. Generalized clonic-tonic seizures were completely suppressed and cortical energy metabolite changes which normally accompany these seizures were either normalized (decrease of glucose and glycogen) or markedly reduced (an accumulation of lactate). EEG recordings support the marked anticonvulsant effect of 2R,4R-APDC, nevertheless, this was only partial. In spite of the absence of obvious motor phenomena, isolated spikes or even short periods of partial ictal activity could be observed. Isolated spikes could also be seen in some animals after application of 2R,4R-APDC alone, reflecting most likely subclinical proconvulsant activity of this agonist. The neuroprotective effect of 2R,4R-APDC was evaluated after 24 h and 6 days of survival following DL-HCA-induced seizures. Massive neuronal degeneration, as revealed by Fluoro-Jade B staining, was observed in a number of brain regions following infusion of DL-HCA alone (seizure group), whereas 2R,4R-APDC pretreatment provided substantial neuroprotection. The present findings support the possibility that group II mGluRs are a promising target for a novel approach to treating epilepsy.

Kinetics and formation mechanisms of intragranular ferrite in V-N microalloyed 600 MPa high strength rebar steel

NASA Astrophysics Data System (ADS)

Zhang, Jing; Wang, Fu-ming; Li, Chang-rong

2016-04-01

To systematically investigate the kinetics and formation mechanisms of intragranular ferrite (IGF), isothermal heat treatment in the temperature range of 450°C to 600°C with holding for 30 s to 300 s, analysis of the corresponding microstructures, and observation of the precipitated particles were conducted in V-N microalloyed 600 MPa high strength rebar steel. The potency of V(C,N) for IGF nucleation was also analyzed statistically. The results show that the dominant microstructure transforms from bainite (B) and acicular ferrite (AF) to grain boundary ferrite (GBF), intragranular polygonal ferrite (IPF), and pearlite (P) as the isothermal temperature increases from 450°C to 600°C. When the holding time at 600°C is extended from 30 s to 60 s, 120 s, and 300 s, the GBF content ranges from 6.0vol% to 6.5vol% and the IPF content increases from 0.5vol% to 2.8vol%, 13.1vol%, and 13.5vol%, respectively, because the ferrite transformation preferentially occurs at the grain boundaries and then occurs at the austenite grains. Notably, V(C,N) particles are the most effective nucleation site for the formation of IPF, accounting for 51% of the said formation.

Effect of increased HoxB4 on human megakaryocytic development

PubMed Central

Zhong, Yiming; Sullenbarger, Brent; Lasky, Larry C.

2010-01-01

In order to ex vivo produce clinically useful quantity of platelets, we may need to firstly enhance early self-renewal of hematopoietic stem cells (HSCs) and/or megakaryocyte (Mk) progenitors. The homeodomain transcription factor HoxB4 has been shown to be an important regulator of stem cell renewal and hematopoiesis; however, its effect on megakaryopoiesis is unclear. In this study, we investigated the effect of HoxB4 overexpression or RNA silencing on megakaryocytic development in the human TF1 progenitor cell line; we then used recombinant tPTD-HoxB4 fusion protein to study the effect of exogenous HoxB4 on megakaryocytic development of human CD34 positively-selected cord blood cells. We found that ectopic HoxB4 in TF1 cells increased the antigen expression of CD61and CD41a, increased the gene expression of thrombopoietin receptor (TpoR), Scl-1, Cyclin D1, Fog-1 and Fli-1 while it decreased c-Myb expression. HoxB4 RNA silencing in TF1 cells decreased the expression of CD61 and CD41a and decreased Fli-1 expression while it increased the expression of c-Myb. Recombinant tPTD-HoxB4 fusion protein increased the percentages and absolute numbers of CD41a and CD61 positive cells during megakaryocytic differentiation of CD34 positively-selected cord blood cells and increased the numbers of colony forming unit-megakaryocyte (CFU-Mk). Adding tPTD-HoxB4 fusion protein increased the gene expression of TpoR, Cyclin D1, Fog-1 and Fli-1 while it inhibited c-Myb expression. Our data indicate that increased HoxB4 enhanced early megakaryocytic development in human TF1 cells and CD34 positively-selected cord blood cells primarily by upregulating Tpo R and Fli-1 expression and downregulating c-Myb expression. Increasing HoxB4 expression or adding recombinant HoxB4 protein might be a way to expand Mks for the production of platelets for use in transfusion medicine. PMID:20599537

The PP4R1 sub-unit of protein phosphatase PP4 is essential for inhibition of NF-κB by merkel polyomavirus small tumour antigen.

PubMed

Abdul-Sada, Hussein; Müller, Marietta; Mehta, Rajni; Toth, Rachel; Arthur, J Simon C; Whitehouse, Adrian; Macdonald, Andrew

2017-04-11

Merkel cell carcinoma (MCC) is a highly aggressive skin cancer with a high metastatic potential. The majority of MCC cases are caused by the Merkel cell polyomavirus (MCPyV), through expression of the virus-encoded tumour antigens. Whilst mechanisms attributing tumour antigen expression to transformation are being uncovered, little is known of the mechanisms by which MCPyV persists in the host. We previously identified the MCPyV small T antigen (tAg) as a novel inhibitor of nuclear factor kappa B (NF-kB) signalling and a modulator of the host anti-viral response. Here we demonstrate that regulation of NF-kB activation involves a previously undocumented interaction between tAg and regulatory sub-unit 1 of protein phosphatase 4 (PP4R1). Formation of a complex with PP4R1 and PP4c is required to bridge MCPyV tAg to the NEMO adaptor protein, allowing deactivation of the NF-kB pathway. Mutations in MCPyV tAg that fail to interact with components of this complex, or siRNA depletion of PP4R1, prevents tAg-mediated inhibition of NF-kB and pro-inflammatory cytokine production. Comparison of tAg binding partners from other human polyomavirus demonstrates that interactions with NEMO and PP4R1 are unique to MCPyV. Collectively, these data identify PP4R1 as a novel target for virus subversion of the host anti-viral response.

17 CFR 301.300b - Form 300-B, for report of all fails to deliver.

Code of Federal Regulations, 2012 CFR

2012-04-01

... fails to deliver. 301.300b Section 301.300b Commodity and Securities Exchanges SECURITIES AND EXCHANGE... Closeout Or Completion of Open Contractual Commitments § 301.300b Form 300-B, for report of all fails to... form shall be used to report all the fails to deliver, as defined by § 300.300(b) of this chapter, that...

17 CFR 301.300b - Form 300-B, for report of all fails to deliver.

Code of Federal Regulations, 2011 CFR

2011-04-01

... fails to deliver. 301.300b Section 301.300b Commodity and Securities Exchanges SECURITIES AND EXCHANGE... Closeout Or Completion of Open Contractual Commitments § 301.300b Form 300-B, for report of all fails to... form shall be used to report all the fails to deliver, as defined by § 300.300(b) of this chapter, that...

17 CFR 301.300b - Form 300-B, for report of all fails to deliver.

Code of Federal Regulations, 2014 CFR

2014-04-01

... fails to deliver. 301.300b Section 301.300b Commodity and Securities Exchanges SECURITIES AND EXCHANGE... Closeout Or Completion of Open Contractual Commitments § 301.300b Form 300-B, for report of all fails to... form shall be used to report all the fails to deliver, as defined by § 300.300(b) of this chapter, that...

17 CFR 301.300b - Form 300-B, for report of all fails to deliver.

Code of Federal Regulations, 2013 CFR

2013-04-01

... fails to deliver. 301.300b Section 301.300b Commodity and Securities Exchanges SECURITIES AND EXCHANGE... Closeout Or Completion of Open Contractual Commitments § 301.300b Form 300-B, for report of all fails to... form shall be used to report all the fails to deliver, as defined by § 300.300(b) of this chapter, that...

Degradation of 4-chlorophenol and microbial diversity in soil inoculated with single Pseudomonas sp. CF600 and Stenotrophomonas maltophilia KB2.

PubMed

Nowak, Agnieszka; Mrozik, Agnieszka

2018-06-01

Soil contamination with chlorophenols is a serious problem all over the world due to their common use in different branches of industry and agriculture. The objective of this study was to determine whether bioaugmenting soil with single Pseudomonas sp. CF600 and Stenotrophomonas maltophilia KB2 and additional carbon sources such as phenol (P) and sodium benzoate (SB) could enhance the degradation of 4-chlorophenol (4-CP). During the degradation experiment, the number of bacteria as well as the structural and functional diversity of the soil microbial communities were determined. It was found that the most effective degradation of 4-CP in the soil was observed after it was inoculated with CF600 and the addition of SB. The biodegradation of five doses of 4-CP in this soil proceeded within 100 days. At the same time, the rate of the disappearance of 4-CP in the soil that had been bioaugmented with CF600 and contaminated with 4-CP and P was 5-6.5 times lower compared to its rate of disappearance in the soil that had been contaminated with 4-CP. The biodegradation of 4-CP in all of the treated and untreated soils was accompanied by a systematic decrease in the number of heterotrophic bacteria (THB) ranging between 13 and 40%. It was also proven that the tested aromatic compounds affected the soil microbial community structure through an increase in the marker fatty acids for Gram-negative bacteria (BG-) and fungi (F). The essential changes in the patterns of the fatty acid methyl esters (FAMEs) for the polluted soil included an increase in the fatty acid saturation and hydroxy fatty acid abundance. The obtained results also indicated that the introduction of CF600 into the soil contaminated with 4-CP and SB or P caused an increase in the functional diversity of the soil microorganisms. In contrast, in the soil that had been inoculated with KB2 and in the non-inoculated soil, the addition of 4-CP and P decreased the microbial activity. In conclusion, the inoculation of

10 CFR 600.321 - Real property and equipment.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Real property and equipment. 600.321 Section 600.321....321 Real property and equipment. (a) Prior approvals for acquisition with Federal funds. Recipients may purchase real property or equipment in whole or in part with Federal funds under an award only...

10 CFR 600.321 - Real property and equipment.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Real property and equipment. 600.321 Section 600.321....321 Real property and equipment. (a) Prior approvals for acquisition with Federal funds. Recipients may purchase real property or equipment in whole or in part with Federal funds under an award only...

10 CFR 600.321 - Real property and equipment.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Real property and equipment. 600.321 Section 600.321....321 Real property and equipment. (a) Prior approvals for acquisition with Federal funds. Recipients may purchase real property or equipment in whole or in part with Federal funds under an award only...

10 CFR 600.321 - Real property and equipment.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Real property and equipment. 600.321 Section 600.321....321 Real property and equipment. (a) Prior approvals for acquisition with Federal funds. Recipients may purchase real property or equipment in whole or in part with Federal funds under an award only...

MicroRNA-300 Regulates the Ubiquitination of PTEN through the CRL4BDCAF13 E3 Ligase in Osteosarcoma Cells.

PubMed

Chen, Zhi; Zhang, Wei; Jiang, Kaibiao; Chen, Bin; Wang, Kun; Lao, Lifeng; Hou, Canglong; Wang, Fei; Zhang, Caiguo; Shen, Hongxing

2018-03-02

Cullins, critical members of the cullin-RING ubiquitin ligases (CRLs), are often aberrantly expressed in different cancers. However, the underlying mechanisms regarding aberrant expression of these cullins and the specific substrates of CRLs in different cancers are mostly unknown. Here, we demonstrate that overexpressed CUL4B in human osteosarcoma cells forms an E3 complex with DNA damage binding protein 1 (DDB1) and DDB1- and CUL4-associated factor 13 (DCAF13). In vitro and in vivo analyses indicated that the CRL4B DCAF13 E3 ligase specifically recognized the tumor suppressor PTEN (phosphatase and tensin homolog deleted on chromosome 10) for degradation, and disruption of this E3 ligase resulted in PTEN accumulation. Further analyses indicated that miR-300 directly targeted the 3' UTR of CUL4B, and DNA hypermethylation of a CpG island in the miR-300 promoter region contributed to the downregulation of miR-300. Interestingly, ectopic expression of miR-300 or treatment with 5-AZA-2'-deoxycytidine, a DNA methylation inhibitor, decreased the stability of CRL4B DCAF13 E3 ligase and reduced PTEN ubiquitination. By applying in vitro screening to identify small molecules that specifically inhibit CUL4B-DDB1 interaction, we found that TSC01131 could greatly inhibit osteosarcoma cell growth and could disrupt the stability of the CRL4B DCAF13 E3 ligase. Collectively, our findings shed new light on the molecular mechanism of CUL4B function and might also provide a new avenue for osteosarcoma therapy. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

40 CFR 600.501-86 - General applicability.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Year 1978 Passenger Automobiles and for 1979 and Later Model Year Automobiles (Light Trucks and Passenger Automobiles)-Procedures for Determining Manufacturer's Average Fuel Economy § 600.501-86 General... and diesel automobiles. (b)(1) Manufacturers that produce only electric vehicles are exempt from the...

40 CFR 600.501-85 - General applicability.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Year 1978 Passenger Automobiles and for 1979 and Later Model Year Automobiles (Light Trucks and Passenger Automobiles)-Procedures for Determining Manufacturer's Average Fuel Economy § 600.501-85 General... applicable to 1985 and later model year gasoline-fueled and diesel automobiles. (b)(1) Manufacturers that...

10 CFR 600.321 - Real property and equipment.

Code of Federal Regulations, 2010 CFR

2010-01-01

... following: (1) During the time that the real property or equipment is used on the project or program for... 10 Energy 4 2010-01-01 2010-01-01 false Real property and equipment. 600.321 Section 600.321....321 Real property and equipment. (a) Prior approvals for acquisition with Federal funds. Recipients...

10 CFR 600.306 - Metric system of measurement.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Metric system of measurement. 600.306 Section 600.306... system of measurement. (a) The Metric Conversion Act of 1975, as amended by the Omnibus Trade and... system is the preferred measurement system for U.S. trade and commerce. (2) The metric system of...

10 CFR 600.306 - Metric system of measurement.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Metric system of measurement. 600.306 Section 600.306... system of measurement. (a) The Metric Conversion Act of 1975, as amended by the Omnibus Trade and... system is the preferred measurement system for U.S. trade and commerce. (2) The metric system of...

10 CFR 600.306 - Metric system of measurement.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Metric system of measurement. 600.306 Section 600.306... system of measurement. (a) The Metric Conversion Act of 1975, as amended by the Omnibus Trade and... system is the preferred measurement system for U.S. trade and commerce. (2) The metric system of...

10 CFR 600.306 - Metric system of measurement.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Metric system of measurement. 600.306 Section 600.306... system of measurement. (a) The Metric Conversion Act of 1975, as amended by the Omnibus Trade and... system is the preferred measurement system for U.S. trade and commerce. (2) The metric system of...

10 CFR 600.306 - Metric system of measurement.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 4 2013-01-01 2013-01-01 false Metric system of measurement. 600.306 Section 600.306... system of measurement. (a) The Metric Conversion Act of 1975, as amended by the Omnibus Trade and... system is the preferred measurement system for U.S. trade and commerce. (2) The metric system of...

MCNP5 CALCULATIONS REPLICATING ARH-600 NITRATE DATA

DOE Office of Scientific and Technical Information (OSTI.GOV)

FINFROCK SH

This report serves to extend the previous document: 'MCNP Calculations Replicating ARH-600 Data' by replicating the nitrate curves found in ARH-600. This report includes the MCNP models used, the calculated critical dimension for each analyzed parameter set, and the resulting data libraries for use with the CritView code. As with the ARH-600 data, this report is not meant to replace the analysis of the fissile systems by qualified criticality personnel. The M CNP data is presented without accounting for the statistical uncertainty (although this is typically less than 0.001) or bias and, as such, the application of a reasonable safetymore » margin is required. The data that follows pertains to the uranyl nitrate and plutonium nitrate spheres, infinite cylinders, and infinite slabs of varying isotopic composition, reflector thickness, and molarity. Each of the cases was modeled in MCNP (version 5.1.40), using the ENDF/B-VI cross section set. Given a molarity, isotopic composition, and reflector thickness, the fissile concentration and diameter (or thicknesses in the case of the slab geometries) were varied. The diameter for which k-effective equals 1.00 for a given concentration could then be calculated and graphed. These graphs are included in this report. The pages that follow describe the regions modeled, formulas for calculating the various parameters, a list of cross-sections used in the calculations, a description of the automation routine and data, and finally the data output. The data of most interest are the critical dimensions of the various systems analyzed. This is presented graphically, and in table format, in Appendix B. Appendix C provides a text listing of the same data in a format that is compatible with the CritView code. Appendices D and E provide listing of example Template files and MCNP input files (these are discussed further in Section 4). Appendix F is a complete listing of all of the output data (i.e., all of the analyzed dimensions and

40 CFR 600.107-78 - Fuel specifications.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Exhaust Emission Regulations for 1978 and Later Model Year Automobiles-Test Procedures § 600.107-78 Fuel specifications. (a) The test fuel specifications for gasoline-fueled automobiles are given in paragraph (a)(1) of § 86.113 of this chapter. (b) The test fuel specifications for diesel automobiles are given in...

10 CFR 600.341 - Monitoring and reporting program and financial performance.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Monitoring and reporting program and financial performance. 600.341 Section 600.341 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL... Organizations Post-Award Requirements § 600.341 Monitoring and reporting program and financial performance. (a...

Dual inhibition of human type 4 phosphodiesterase isostates by (R, R)-(+/-)-methyl 3-acetyl-4-[3-(cyclopentyloxy)-4-methoxyphenyl]-3- methyl-1-pyrrolidinecarboxylate.

PubMed

Tian, G; Rocque, W J; Wiseman, J S; Thompson, I Z; Holmes, W D; Domanico, P L; Stafford, J A; Feldman, P L; Luther, M A

1998-05-12

Purified recombinant human type 4 phosphodiesterase B2B (HSPDE4B2B) exists in both a low- and a high-affinity state that bind (R)-rolipram with Kd's of ca. 500 and 1 nM, respectively [Rocque, W. J., Tian, G., Wiseman, J. S., Holmes, W. D., Thompson, I. Z., Willard, D. H., Patel, I. R., Wisely, G. B., Clay, W. C., Kadwell, S. H., Hoffman, C. R., and Luther, M. A. (1997) Biochemistry 36, 14250-14261]. Since the tissue distribution of the two isostates may be significantly different, development of inhibitors that effectively inhibit both forms may be advantageous pharmacologically. In this study, enzyme inhibition and binding of HSPDE4B2B by (R, R)-(+/-)-methyl 3-acetyl-4-[3-(cyclopentyloxy)-4-methoxyphenyl]-3-methyl-1-pyrrolidin ecarboxylate (1), a novel inhibitor of phosphodiesterase 4 (PDE 4), were investigated. Binding experiments demonstrated high-affinity binding of 1 to HSPDE4B2B with a stoichiometry of 1:1. Inhibition of PDE activity showed only a single transition with an observed Ki similar to the apparent Kd determined by the binding experiments. Deletional mutants of HSPDE4B2B, which have been shown to bind (R)-rolipram with low affinity, were shown to interact with 1 with high affinity, indistinguishable from the results obtained with the full-length enzyme. Bound 1 was completely displaced by (R)-rolipram, and the displacement showed a biphasic transition that resembles the biphasic inhibition of HSPDE4B2B by (R)-rolipram. Theoretical analysis of the two transitions exemplified in the interaction of (R)-rolipram with HSPDE4B2B indicated that the two isostates were nonexchangeable. Phosphorylation at serines 487 and 489 on HSPDE4B2B had no effect on the stoichiometry of binding, the affinity for binding, or the inhibition of the enzyme by 1. These data further illustrate the presence of two isostates in PDE 4 as shown previously for (R)-rolipram binding and inhibition. In contrast to (R)-rolipram, where only one of the two isostates of PDE 4 binds with

10 CFR 600.121 - Standards for financial management systems.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Standards for financial management systems. 600.121... Education, Hospitals, and Other Nonprofit Organizations Post-Award Requirements § 600.121 Standards for financial management systems. (a) Recipients shall relate financial data to performance data and develop...

10 CFR 600.121 - Standards for financial management systems.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Standards for financial management systems. 600.121 Section 600.121 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES... financial management systems. (a) Recipients shall relate financial data to performance data and develop...

10 CFR 600.5 - Selection of award instrument.

Code of Federal Regulations, 2010 CFR

2010-01-01

... instrument, the DOE decision as to whether the relationship is principally one of procurement or financial... financial assistance instrument, DOE shall limit involvement between itself and the recipient in the... 10 Energy 4 2010-01-01 2010-01-01 false Selection of award instrument. 600.5 Section 600.5 Energy...

24 CFR 87.600 - Semi-annual compilation.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Semi-annual compilation. 87.600... the six-month period ending on March 31 or September 30, respectively, of that year. (b) The report..., and shall contain a compilation of the disclosure reports received from December 23, 1989 to March 31...

Ag3PO4 nanocrystals deposited on monoclinic olive-like BiVO4 with efficient photodegradation of organic dyes under visible light irradiation

NASA Astrophysics Data System (ADS)

Chen, Jingshuai; Jiang, Liang-Liang; Liu, Xing-Pei; Mao, Chang-Jie; Song, Ji-Ming; Niu, Helin; Zhang, Shengyi

2017-05-01

Olive-like BiVO4 microstructures with lengths of 600-1000 nm and widths of 300-600 nm have been synthesized via a facile and additive-free solvothermal method. Studies find that the type of solvent plays an important role in the morphology of the final products. Furthermore, Ag3PO4 nanocrystals are successfully deposited on monoclinic olive-like BiVO4 via in situ precipitation method. The as-synthesized samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high resolution transmission electron microscopy (HRTEM), photoluminescence (PL) spectra, and UV-vis diffuse reflectance spectra (DRS). The photocatalytic activities of the catalysts are evaluated by degradation of rhodamine B (RhB) and methylene blue (MB) under visible light (≥420 nm) irradiation. The experimental results suggest that catalytic activity of the composite photocatalysts is greatly influenced by the loading level of Ag3PO4. The molar ratio of 0.8:1.0 Ag3PO4-loaded BiVO4 exhibits higher photocatalytic activity in both the decolorization of RhB and MB than that of individual BiVO4 and P25. The observed improvement in photocatalytic activity is associated with the extended absorption in the visible light region resulting from the Ag3PO4 nanoparticles, and the effective separation of photogenerated carriers at the Ag3PO4/BiVO4 interfaces through the formation of heterojunction structure. The study provides a general and effective method in the fabrication of composite with sound heterojunctions that may show a variety of applications.

40 CFR 600.107-93 - Fuel specifications.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Exhaust Emission Regulations for 1978 and Later Model Year Automobiles-Test Procedures § 600.107-93 Fuel specifications. (a) The test fuel specifications for gasoline-fueled automobiles are given in § 86.113(a) (1) and (2) of this chapter. (b) The test fuel specifications for diesel-fueled automobiles are given in § 86...

10 CFR 600.311 - Standards for financial management systems.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Standards for financial management systems. 600.311... Requirements § 600.311 Standards for financial management systems. (a) Recipients are encouraged to use existing financial management systems to the extent that the systems comply with Generally Accepted...

10 CFR 600.311 - Standards for financial management systems.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Standards for financial management systems. 600.311... Requirements § 600.311 Standards for financial management systems. (a) Recipients are encouraged to use existing financial management systems to the extent that the systems comply with Generally Accepted...

10 CFR 600.340 - Purpose of reports and records.

Code of Federal Regulations, 2010 CFR

2010-01-01

... and reporting financial and program performance and for records retention. ... 10 Energy 4 2010-01-01 2010-01-01 false Purpose of reports and records. 600.340 Section 600.340 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Administrative...

10 CFR 600.220 - Standards for financial management systems.

Code of Federal Regulations, 2011 CFR

2011-01-01

... subgrant award documents, etc. (7) Cash management. Procedures for minimizing the time elapsing between the... 10 Energy 4 2011-01-01 2011-01-01 false Standards for financial management systems. 600.220... Post-Award Requirements § 600.220 Standards for financial management systems. (a) A State must expend...

40 CFR 600.306-86 - Labeling requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... labels are required to bear range values as required by paragraph (b) of this section, or determined by... requested which has a combined unadjusted fuel economy value at or below the minimum tax-free value, the... manufacturer shall include the current range of fuel economy of comparable automobiles (as described in §§ 600...

40 CFR 600.306-86 - Labeling requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... labels are required to bear range values as required by paragraph (b) of this section, or determined by... requested which has a combined unadjusted fuel economy value at or below the minimum tax-free value, the... manufacturer shall include the current range of fuel economy of comparable automobiles (as described in §§ 600...

Enhanced supercapacitive behaviour of Fe3O4/fMWCNT nanoassemblies synthesized by PEG-600 assisted solvothermal method

NASA Astrophysics Data System (ADS)

Aparna, M. L.; Sathyanarayanan, P.; Sahu, Niroj Kumar

2018-04-01

We report a simple PEG-600 assisted solvothermal method for the synthesis iron ferrite with functionalized multi-walled carbon nanotube (Fe3O4/fMWCNT) composite nanoassemblies. The results show that the composite deliver excellent electrochemical activity because of the synergistic effect of each component. The fMWCNT act as a conductive network with high surface area promoting fast movement of electrons which enhances the charge storing nature and stability of Fe3O4 nanoassemblies.

DRV concentrations and viral load in CSF in patients on DRV/r 600/100 or 800/100mg once daily plus two NRTI.

PubMed

Di Yacovo, Silvana; Molto, Jose; Ferrer, Elena; Curran, Adrian; Else, Laura Jayne; Clotet, Bonaventura; Tiraboschi, Juan; Niubo, Jordi; Vila, Antonia; Podzamczer, Daniel

2014-01-01

Darunavir/r (DRV/r) is currently used at a dose of 800/100 mg once daily (OD) in a high proportion of patients. Pharmacokinetic data suggest that 600/100 OD may be effective, reducing toxicity and cost. However, drug concentrations in reservoirs such as cerebrospinal fluid (CSF) might not be adequate to inhibit viral replication. We aimed to evaluate concentrations of DRV and HIV-1 viral load (VL) in CSF patients receiving DRV 600/100 mg OD. DRV600 is an ongoing randomized open study comparing DRV/r 800/100 mg (DRV800) vs 600/100 mg (DRV600) OD plus TDF/FTC or ABC/3TC in 100 virologically suppressed patients (eudraCT 2011-006272-39). Here we present the results of a CSF sub-study. A lumbar puncture (LP) was performed in participating patients after at least six months of inclusion in the study, 20-28 hours after a dose of DRV/r. VL (PCR, LOD 40 copies/mL) was determined in CSF and in plasma. DRV concentrations were quantified in CSF by liquid chromatography mass spectrometry (LC/MS/MS) and in plasma using high-performance liquid chromatography (HPLC). Sixteen patients were included (eight in each arm). All DRV600 patients and four out of eight DRV800 patients received TDF/FTC, and the other four ABC/3TC. 75% were males, median (range) age was 48 (17-71) years, CD4 cell count 532 cells/mL (190-1,394). Median total time on DRV/r was 30 (11-57) months, and since the beginning of the study 8 (6-12) months in DRV800 and 10 (7-12) months in DRV600 patients. LP was performed a median of 26 (24-28) hours after the last DRV/r+TVD or KVX dose. In DRV600 patients the median DRV plasma levels were 1,674 (326-3,742) ng/mL, CSF levels 17.08 (5.79-30.19) ng/mL and DRV CSF:plasma ratio 0.0084 (0.0028-0.0388), while in the DRV800 arm, median DRV plasma levels were 1,707 (958-3,910) ng/mL, CSF levels 13.23 (3.47-32.98) ng/mL and DRV CSF:plasma ratio 0.0104 (0.0018-0.0262). All patients had VL<40 copies/mL in plasma and 14 patients VL<40 copies/mL in CSF. Two patients (1 in each arm

10 CFR 600.504 - Information an applicant must submit.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 4 2012-01-01 2012-01-01 false Information an applicant must submit. 600.504 Section 600... Information an applicant must submit. (a) Any applicant for financial assistance under a covered program shall... United States of products resulting from a technology developed with financial assistance or to procure...

10 CFR 600.504 - Information an applicant must submit.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 4 2014-01-01 2014-01-01 false Information an applicant must submit. 600.504 Section 600... Information an applicant must submit. (a) Any applicant for financial assistance under a covered program shall... United States of products resulting from a technology developed with financial assistance or to procure...

10 CFR 600.504 - Information an applicant must submit.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 4 2011-01-01 2011-01-01 false Information an applicant must submit. 600.504 Section 600... Information an applicant must submit. (a) Any applicant for financial assistance under a covered program shall... United States of products resulting from a technology developed with financial assistance or to procure...

Characterization of B4C-composite-reinforced aluminum alloy composites

NASA Astrophysics Data System (ADS)

Singh, Ram; Rai, R. N.

2018-04-01

Dry sliding wear tests conducted on Pin-on-disk wear test machine. The rotational speed of disc is ranging from (400-600rpm) and under loads ranging from (30-70 N) the contact time between the disc and pin is constant for each pin specimen of composites is 15 minute. In all manufacturing industries the uses of composite materials has been increasing globally, In the present study, an aluminum 5083 alloy is used as the matrix and 5% of weight percentage of Boron Carbide (B4C) as the reinforcing material. The composite is produced using stir casting technique. This is cost effective method. The aluminum 5083 matrix can be strengthened by reinforcing with hard ceramic particles like silicon carbide and boron carbide. In this experiment, aluminum 5083 alloy is selected as one of main material for making parts of the ship it has good mechanical properties, good corrosion resistance and it is can welded very easily and does have good strength. The samples are tested for hardness and tensile strength. The mechanical properties like Hardness can be increased by reinforcing aluminum 5083alloy 5% boron carbide (B4C) particles and tensile strength. Finally the Scanning Electron Microscope (SEM) analysis and EDS is done, which helps to study topography of composites and it produces images of a sample by scanning it with a focused beam of electrons and the presence of composition found in the matrix.

33 CFR 207.600 - Rochester (Charlotte) Harbor, N.Y.; use, administration, and navigation.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Rochester (Charlotte) Harbor, N.Y.; use, administration, and navigation. 207.600 Section 207.600 Navigation and Navigable Waters CORPS OF... (Charlotte) Harbor, N.Y.; use, administration, and navigation. (a)-(b) [Reserved] (c) No vessel shall moor or...

10 CFR 600.135 - Supplies and other expendable property.

Code of Federal Regulations, 2010 CFR

2010-01-01

... acquisition. If there is a residual inventory of unused supplies exceeding $5000 in total aggregate value upon... 10 Energy 4 2010-01-01 2010-01-01 false Supplies and other expendable property. 600.135 Section... Education, Hospitals, and Other Nonprofit Organizations Post-Award Requirements § 600.135 Supplies and other...

10 CFR 600.220 - Standards for financial management systems.

Code of Federal Regulations, 2010 CFR

2010-01-01

... financial reporting requirements of the grant or subgrant. (2) Accounting records. Grantees and subgrantees... 10 Energy 4 2010-01-01 2010-01-01 false Standards for financial management systems. 600.220 Section 600.220 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES...

miR-188-5p inhibits tumour growth and metastasis in prostate cancer by repressing LAPTM4B expression

PubMed Central

Zhang, Hongtuan; Qi, Shiyong; Zhang, Tao; Wang, Andi; Liu, Ranlu; Guo, Jia; Wang, Yuzhuo; Xu, Yong

2015-01-01

Elucidation of the molecular targets and pathways regulated by the tumour-suppressive miRNAs can shed light on the oncogenic and metastatic processes in prostate cancer (PCa). Using miRNA profiling analysis, we find that miR-188-5p was significantly down-regulated in metastatic PCa. Down-regulation of miR-188-5p is an independent prognostic factor for poor overall and biochemical recurrence-free survival. Restoration of miR-188-5p in PCa cells (PC-3 and LNCaP) significantly suppresses proliferation, migration and invasion in vitro and inhibits tumour growth and metastasis in vivo. We also find overexpression of miR-188-5p in PC-3 cells can significantly enhance the cells' chemosensitivity to adriamycin. LAPTM4B is subsequently identified as a direct target of miR-188-5p in PCa, and is found to be significantly over-expressed in PCa. Knockdown of LAPTM4B phenotypically copies miR-188-5p-induced phenotypes, whereas ectopic expression of LAPTM4B reverses the effects of miR-188-5p. We also find that restoration of miR-188-5p can inhibit the PI3K/AKT signaling pathway via the suppression of LAPTM4B. Taken together, this is the first report unveils that miR-188-5p acts as a tumour suppressor in PCa and may therefore serve as a useful therapeutic target for the development of new anticancer therapy. PMID:25714029

miR-188-5p inhibits tumour growth and metastasis in prostate cancer by repressing LAPTM4B expression.

PubMed

Zhang, Hongtuan; Qi, Shiyong; Zhang, Tao; Wang, Andi; Liu, Ranlu; Guo, Jia; Wang, Yuzhuo; Xu, Yong

2015-03-20

Elucidation of the molecular targets and pathways regulated by the tumour-suppressive miRNAs can shed light on the oncogenic and metastatic processes in prostate cancer (PCa). Using miRNA profiling analysis, we find that miR-188-5p was significantly down-regulated in metastatic PCa. Down-regulation of miR-188-5p is an independent prognostic factor for poor overall and biochemical recurrence-free survival. Restoration of miR-188-5p in PCa cells (PC-3 and LNCaP) significantly suppresses proliferation, migration and invasion in vitro and inhibits tumour growth and metastasis in vivo. We also find overexpression of miR-188-5p in PC-3 cells can significantly enhance the cells' chemosensitivity to adriamycin. LAPTM4B is subsequently identified as a direct target of miR-188-5p in PCa, and is found to be significantly over-expressed in PCa. Knockdown of LAPTM4B phenotypically copies miR-188-5p-induced phenotypes, whereas ectopic expression of LAPTM4B reverses the effects of miR-188-5p. We also find that restoration of miR-188-5p can inhibit the PI3K/AKT signaling pathway via the suppression of LAPTM4B. Taken together, this is the first report unveils that miR-188-5p acts as a tumour suppressor in PCa and may therefore serve as a useful therapeutic target for the development of new anticancer therapy.

EphB4 as a therapeutic target in mesothelioma

PubMed Central

2013-01-01

Background Malignant pleural mesothelioma (MPM) often develops decades following exposure to asbestos. Current best therapy produces a response in only half of patients, and the median survival with this therapy remains under a year. A search for novel targets and therapeutics is underway, and recently identified targets include VEGF, Notch, and EphB4-Ephrin-B2. Each of these targets has dual activity, promoting tumor cell growth as well as tumor angiogenesis. Methods We investigated EphB4 expression in 39 human mesothelioma tissues by immunohistochemistry. Xenograft tumors established with human mesothelioma cells were treated with an EphB4 inhibitor (monomeric soluble EphB4 fused to human serum albumin, or sEphB4-HSA). The combinatorial effect of sEphB4-HSA and biologic agent was also studied. Results EphB4 was overexpressed in 72% of mesothelioma tissues evaluated, with 85% of epithelioid and 38% of sarcomatoid subtypes demonstrating overexpression. The EphB4 inhibitor sEphB4-HSA was highly active as a single agent to inhibit tumor growth, accompanied by tumor cell apoptosis and inhibition of PI3K and Src signaling. Combination of sEphB4-HSA and the anti-VEGF antibody (Bevacizumab) was superior to each agent alone and led to complete tumor regression. Conclusion EphB4 is a potential therapeutic target in mesothelioma. Clinical investigation of sEphB4-HSA as a single agent and in combination with VEGF inhibitors is warranted. PMID:23721559

Map saturation and SNP marker development for the rust resistance genes (R4, R5, R13a, and R13b) in sunflower (Helianthus annuus L.)

USDA-ARS?s Scientific Manuscript database

Sunflower rust, which is incited by the fungus Puccinia helianthi Schwein., is the most common disease in Australia, Argentina, South Africa, and North America. Three independent genes, R5, R4, and R13 with two alleles R13a and R13b, were discovered in sunflower and are promising sources of resistan...

Observation of e + e → Φ χ c 1 and Φ χ c 2 at s = 4.600 GeV

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ablikim, M.; Achasov, M. N.; Ahmed, S.

Using a data sample collected with the BESIII detector operating at the BEPCII storage ring at a centerof- mass energy of √s = 4.600 GeV, we search for the production of e +e - → φχ c0,1,2. A search is also performed for the charmonium-like state X(4140) in the radiative transition e +e - → γX(4140) with X(4140) subsequently decaying into φJ/ψ. The processes e +e - → φχ c1 and φχ c2 are observed for the first time, each with a statistical significance ofmore than 10σ, and the Born cross sections are measuredmore » to be (4.2+1.7 -1.0±0.3) pb and (6.7+3.4 -1.7 ± 0.5) pb, respectively, where the first uncertainties are statistical and the second systematic. No significant signals are observed for e +e - → φχ c0 and e +e - → γX(4140) and upper limits on the Born cross sections at 90% confidence level are provided at √s = 4.600 GeV.« less

Observation of e + e → Φ χ c 1 and Φ χ c 2 at s = 4.600 GeV

DOE PAGES

Ablikim, M.; Achasov, M. N.; Ahmed, S.; ...

2018-02-12

Using a data sample collected with the BESIII detector operating at the BEPCII storage ring at a centerof- mass energy of √s = 4.600 GeV, we search for the production of e +e - → φχ c0,1,2. A search is also performed for the charmonium-like state X(4140) in the radiative transition e +e - → γX(4140) with X(4140) subsequently decaying into φJ/ψ. The processes e +e - → φχ c1 and φχ c2 are observed for the first time, each with a statistical significance ofmore than 10σ, and the Born cross sections are measuredmore » to be (4.2+1.7 -1.0±0.3) pb and (6.7+3.4 -1.7 ± 0.5) pb, respectively, where the first uncertainties are statistical and the second systematic. No significant signals are observed for e +e - → φχ c0 and e +e - → γX(4140) and upper limits on the Born cross sections at 90% confidence level are provided at √s = 4.600 GeV.« less

Effect of hot-dip galvanizing processes on the microstructure and mechanical properties of 600-MPa hot-dip galvanized dual-phase steel

NASA Astrophysics Data System (ADS)

Kuang, Chun-fu; Zheng, Zhi-wang; Wang, Min-li; Xu, Quan; Zhang, Shen-gen

2017-12-01

A C-Mn dual-phase steel was soaked at 800°C for 90 s and then either rapidly cooled to 450°C and held for 30 s (process A) or rapidly cooled to 350°C and then reheated to 450°C (process B) to simulate the hot-dip galvanizing process. The influence of the hot-dip galvanizing process on the microstructure and mechanical properties of 600-MPa hot-dip galvanized dual-phase steel (DP600) was investigated using optical microscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and tensile tests. The results showed that, in the case of process A, the microstructure of DP600 was composed of ferrite, martensite, and a small amount of bainite. The granular bainite was formed in the hot-dip galvanizing stage, and martensite islands were formed in the final cooling stage after hot-dip galvanizing. By contrast, in the case of process B, the microstructure of the DP600 was composed of ferrite, martensite, bainite, and cementite. In addition, compared with the yield strength (YS) of the DP600 annealed by process A, that for the DP600 annealed by process B increased by approximately 50 MPa because of the tempering of the martensite formed during rapid cooling. The work-hardening coefficient ( n value) of the DP600 steel annealed by process B clearly decreased because the increase of the YS affected the computation result for the n value. However, the ultimate tensile strength (UTS) and elongation ( A 80) of the DP600 annealed by process B exhibited less variation compared with those of the DP600 annealed by process A. Therefore, DP600 with excellent comprehensive mechanical properties (YS = 362 MPa, UTS = 638 MPa, A 80 = 24.3%, n = 0.17) was obtained via process A.

17 CFR 301.300b - Form 300-B, for report of all fails to deliver.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Form 300-B, for report of all fails to deliver. 301.300b Section 301.300b Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) Schedule A to Part 285 FORMS, SECURITIES INVESTOR PROTECTION CORPORATION Forms for...

A facile hydrothermal approach to synthesize rGO/BiVO4 photocatalysts for visible light induced degradation of RhB dye

NASA Astrophysics Data System (ADS)

Pal, Shreyasi; Dutta, Shibsankar; De, Sukanta

2018-05-01

RGO/BiVO4 composites were synthesized by a simple hydrothermal method. The samples were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) and high-resolution transmission electron microscopy (HRTEM) and surface analysis (BET). The photocatalytic activity of the as-prepared samples was evaluated by studying the degradation of model dyes rhodamine B (RhB) under visible light. The prepared rGO/BiVO4 composites exhibited higher photocatalytic activity for the degradation of RhB with a maximum removal rate of 86% under visible light irradiation under visible-light irradiation than pure BiVO4 nanoparticles (63%). This behavior could be associated to their higher specific surface area (BET), increased light absorption intensity and the degradation of electron-hole pair recombination in BiVO4 with the introduction of the rGO.

miR-146b-5p promotes the neural conversion of pluripotent stem cells by targeting Smad4

PubMed Central

Zhang, Nianping; Lyu, Ying; Pan, Xuebing; Xu, Liping; Xuan, Aiguo; He, Xiaosong; Huang, Wandan; Long, Dahong

2017-01-01

Pluripotent stem cells (PSCs) are regarded as potential sources that provide specific neural cells for cell therapy in some nervous system diseases. However, the mechanisms underlying the neural differentiation of PSCs remain largely unknown. MicroRNAs (miRNAs or miRs) are a class of small non-protein-coding RNAs that act as critical regulatory molecules in many cellular processes. In this study, we found that miR-146b-5p expression was markedly increased following the neural induction of mouse embryonic stem cells (ESCs) or induced PSCs (iPSCs). In this study, to further identify the role of miR-146b-5p, we generated stable miR-146b-5p- overexpressing ESC and iPSC cell lines, and induced the differentiation of these cells by the adherent monolayer culture method. In the miR-146b-5p-overexpressing ESC- or iPSC- derived cultures, RT-qPCR analysis revealed that the mRNA expression levels of neuroectoderm markers, such as Sox1, Nestin and Pax6, were markedly increased, and flow cytometric analysis verified that the number of Nestin-positive cells was higher in the miR-146b-5p-overexpressing compared with the control cells. Mechanistically, the miR-146b-5p-overexpressing ESCs or iPSCs exhibited a significant reduction in Oct4 expression, which may be an explanation for these cells having a tendency to differentiate towards the neural lineage. Moreover, we confirmed that miR-146b-5p directly targeted Smad4 and negatively regulated the transforming growth factor (TGF)-β signaling pathway, which contributed to the neural commitment of PSCs. Collectively, our findings uncover the essential role of miR-146b-5p in the neural conversion of PSCs. PMID:28713933

CASMO5 JENDL-4.0 and ENDF/B-VII.1beta4 libraries

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rhodes, J.; Gheorghiu, N.; Ferrer, R.

2012-07-01

This paper details the generation of neutron data libraries for the CASMO5 lattice physics code based on the recently released JENDL-4.0 and ENDF/B-VII.1beta4 nuclear data evaluations. This data represents state-of-the-art nuclear data for late-2011. The key features of the new evaluations are briefly described along with the procedure for processing of this data into CASMO5, 586-energy group neutron data libraries. Finally some CASMO5 results for standard UO{sub 2} and MOX critical experiments for the two new libraries and the current ENDF/B-VII.0 CASMO5 library are presented including the B and W 1810 series, DIMPLE S06A, S06B, TCA reflector criticals with ironmore » plates and the PNL-30-35 MOX criticals. The results show that CASMO5 with the new libraries is performing well for these criticals with a very slight edge in results to the JENDL-4.0 nuclear data evaluation over the ENDF/B-VII.1beta4 evaluation. Work is currently underway to generate a CASMO5 library based on the final ENDF/B-VII.R1 evaluation released Dec. 22, 2011. (authors)« less

Plume Characteristics of the BHT-HD-600 Hall Thruster (Preprint)

DTIC Science & Technology

2006-07-01

Hall thruster on spacecraft, a number of plume properties have been measured. These include current density using a Faraday probe, ion energy distribution using a retarding potential analyzer, and ion species fractions using an E x B probe. The BHT-HD-600 Hall thruster is a nominally 600 W xenon Hall thruster developed by Busek Co. Inc. for the U.S. Air Force Research Laboratory. Plume characterization of Hall thrusters is required to fully understand the impacts of thruster operation on spacecraft. Much of these plume data are

Circulating cell-free BRAFV600E as a biomarker in children with Langerhans cell histiocytosis.

PubMed

Héritier, Sébastien; Hélias-Rodzewicz, Zofia; Lapillonne, Hélène; Terrones, Nathalie; Garrigou, Sonia; Normand, Corinne; Barkaoui, Mohamed-Aziz; Miron, Jean; Plat, Geneviève; Aladjidi, Nathalie; Pagnier, Anne; Deville, Anne; Gillibert-Yvert, Marion; Moshous, Despina; Lefèvre-Utile, Alain; Lutun, Anne; Paillard, Catherine; Thomas, Caroline; Jeziorski, Eric; Nizard, Philippe; Taly, Valérie; Emile, Jean-François; Donadieu, Jean

2017-08-01

The BRAF V600E mutation is reported in half of patients with Langerhans cell histiocytosis (LCH). This study investigated the detection of the BRAF V600E allele in circulating cell-free (ccf) DNA in a paediatric LCH cohort. Children with BRAF V600E -mutated LCH were investigated to detect ccf BRAF V600E at diagnosis (n = 48) and during follow-up (n = 17) using a picolitre-droplet digital PCR assay. At diagnosis, ccf BRAF V600E was positive in 15/15 (100%) patients with risk-organ positive multisystem (RO+ MS) LCH, 5/12 (42%) of patients with RO- MS LCH and 3/21 (14%) patients with single-system (SS) LCH (P < 0·001, Fisher's exact test). The positive BRAF V600E load was higher for RO+ patients (mean, 2·90%; range, 0·04-11·4%) than for RO- patients (mean, 0·16%; range, 0·01-0·39) (P = 0·003, Mann-Whitney U test). After first-line vinblastine-steroid induction therapy, 7/7 (100%) of the non-responders remained positive for ccf BRAF V600E compared to 2/4 (50%) of the partial-responders and 0/4 of the complete responders (P = 0·002, Fisher's exact test). Six children treated with vemurafenib showed a clinical response that was associated with a decrease in the ccf BRAF V600E load at day 15. Thus, ccf BRAF V600E is a promising biomarker for monitoring the response to therapy for children with RO+ MS LCH or RO- LCH resistant to first-line chemotherapy. © 2017 John Wiley & Sons Ltd.

The role of BRAF V600 mutation in melanoma

PubMed Central

2012-01-01

BRAF is a serine/threonine protein kinase activating the MAP kinase/ERK-signaling pathway. About 50 % of melanomas harbors activating BRAF mutations (over 90 % V600E). BRAFV600E has been implicated in different mechanisms underlying melanomagenesis, most of which due to the deregulated activation of the downstream MEK/ERK effectors. The first selective inhibitor of mutant BRAF, vemurafenib, after highly encouraging results of the phase I and II trial, was compared to dacarbazine in a phase III trial in treatment-naïve patients (BRIM-3). The study results showed a relative reduction of 63 % in risk of death and 74 % in risk of tumor progression. Considering all trials so far completed, median overall survival reached approximately 16 months for vemurafenib compared to less than 10 months for dacarbazine treatment. Vemurafenib has been extensively tested on melanoma patients expressing the BRAFV600E mutated form; it has been demonstrated to be also effective in inhibiting melanomas carrying the V600K mutation. In 2011, both FDA and EMA therefore approved vemurafenib for metastatic melanoma carrying BRAFV600 mutations. Some findings suggest that continuation of vemurafenib treatment is potentially beneficial after local therapy in a subset of patients with disease progression (PD). Among who continued vemurafenib >30 days after local therapy of PD lesion(s), a median overall survival was not reached, with a median follow-up of 15.5 months from initiation of BRAF inhibitor therapy. For patients who did not continue treatment, median overall survival from the time of disease progression was 1.4 months. A clinical phase I/II trial is evaluating the safety, tolerability and efficacy of vemurafenib in combination with the CTLA-4 inhibitor mAb ipilimumab. In the BRIM-7 trial vemurafenib is tested in association with GDC-0973, a potent and highly selective inhibitor of MEK1/2. Preliminary data seem to indicate that an additional inhibitor of mutated BRAF, GSK2118436, might

75 FR 8549 - Airworthiness Directives; Airbus Model A300 Series Airplanes, Airbus Model A300 B4-600 Series...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-25

... rack 101VU by accomplishment of Airbus Service Bulletin (SB) A310-53-2076 was embodied on the aeroplane... the splicing with rack 107VU fitting. This condition, if not detected and corrected, could degrade the structural integrity of the crossbeam on NLG FR15A web attachment fitting of rack 107VU. Rack 107VU contains...

10 CFR 600.502 - What must DOE determine.

Code of Federal Regulations, 2010 CFR

2010-01-01

... determine. A company shall be eligible to receive an award of financial assistance under a covered program only if DOE finds that— (a) Consistent with § 600.503, the company's participation in a covered program would be in the economic interest of the United States; and (b) The company is either— (1) A United...

THE TRANSIT LIGHT-CURVE PROJECT. XIV. CONFIRMATION OF ANOMALOUS RADII FOR THE EXOPLANETS TrES-4b, HAT-P-3b, AND WASP-12b

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chan, Tucker; Ingemyr, Mikael; Winn, Joshua N.

2011-06-15

We present transit photometry of three exoplanets, TrES-4b, HAT-P-3b, and WASP-12b, allowing for refined estimates of the systems' parameters. TrES-4b and WASP-12b were confirmed to be 'bloated' planets, with radii of 1.706 {+-} 0.056R{sub Jup} and 1.736 {+-} 0.092R{sub Jup}, respectively. These planets are too large to be explained with standard models of gas giant planets. In contrast, HAT-P-3b has a radius of 0.827 {+-} 0.055R{sub Jup}, smaller than a pure hydrogen-helium planet and indicative of a highly metal-enriched composition. Analyses of the transit timings revealed no significant departures from strict periodicity. For TrES-4, our relatively recent observations allow formore » improvement in the orbital ephemerides, which is useful for planning future observations.« less

40 CFR 600.510-12 - Calculation of average fuel economy and average carbon-related exhaust emissions.

Code of Federal Regulations, 2013 CFR

2013-07-01

... nearest 0.1 mpg; or (iii) For natural gas-fueled model types, the fuel economy value calculated for that... as determined in § 600.208-12(b)(5)(i). (vi) For natural gas dual fuel model types, for model years... natural gas as determined in § 600.208-12(b)(5)(ii) divided by 0.15 provided the requirements of paragraph...

40 CFR 600.510-12 - Calculation of average fuel economy and average carbon-related exhaust emissions.

Code of Federal Regulations, 2014 CFR

2014-07-01

... nearest 0.1 mpg; or (iii) For natural gas-fueled model types, the fuel economy value calculated for that... as determined in § 600.208-12(b)(5)(i). (vi) For natural gas dual fuel model types, for model years... natural gas as determined in § 600.208-12(b)(5)(ii) divided by 0.15 provided the requirements of paragraph...

Response of DP 600 products to dynamic impact loads

NASA Astrophysics Data System (ADS)

Clark, Deidra Darcell

The objective of this study was to compare the microstructural response of various DP 600 products subjected to low velocity, dynamic impact tests, typically encountered in a car crash. Since the response of steel is sensitive to its microstructure as controlled by the alloying elements, phase content, and processing; various DP 600 products may respond differently to crashes. The microstructure before and after dynamic impact deformation at 5 and 10 mph was characterized with regards to grain size, morphology, and phase content among vendors A, B, and C to evaluate efficiency in absorbing energy mechanisms during a crash simulated by dynamic impact testing in a drop tower.

miR-2909-mediated regulation of KLF4: a novel molecular mechanism for differentiating between B-cell and T-cell pediatric acute lymphoblastic leukemias

PubMed Central

2014-01-01

Background microRNAs (miRNAs) play both oncogenic and oncostatic roles in leukemia. However, the molecular details underlying miRNA-mediated regulation of their target genes in pediatric B- and T-cell acute lymphoblastic leukemias (ALLs) remain unclear. The present study investigated the relationship between miR-2909 and Kruppel-like factor 4 (KLF4), and its functional relevance to cell cycle progression and immortalization in patients with pediatric ALL. Methods Elevated levels of miR-2909 targeted the tumor suppressor gene KLF4 in pediatric B-cell, but not pediatric T-cell ALL, as detected by pMIR-GFP reporter assay. Expression levels of genes including apoptosis-antagonizing transcription factor (AATF), MYC, B-cell lymphoma (BCL3), P21 CIP , CCND1 and SP1 in B- and T-cells from patients with pediatric ALL were compared with control levels using real-time quantitative reverse transcription polymerase chain reaction, western blotting, and reporter assays. Results We identified two novel mutations in KLF4 in pediatric T-ALL. A mutation in the 3′ untranslated region of the KLF4 gene resulted in loss of miR-2909-mediated regulation, while mutation in its first or third zinc-finger motif (Zf1/Zf3) rendered KLF4 transcriptionally inactive. This mutation was a frameshift mutation resulting in alteration of the Zf3 motif sequence in the mutant KLF4 protein in all pediatric T-ALL samples. Homology models, docking studies and promoter activity of its target gene P21 CIP confirmed the lack of function of the mutant KLF4 protein in pediatric T-ALL. Moreover, the inability of miR-2909 to regulate KLF4 and its downstream genes controlling cell cycle and apoptosis in T-cell but not in B-ALL was verified by antagomiR-2909 transfection. Comprehensive sequence analysis of KLF4 identified the predominance of isoform 1 (~55 kDa) in most patients with pediatric B-ALL, while those with pediatric T-ALL expressed isoform 2 (~51 kDa). Conclusions This study identified a novel miR

BRAF V600 Mutation Profile of Metastatic Melanoma in the Thrace Region of Turkey.

PubMed

Can, Nuray; Taştekin, Ebru; Deniz Yalta, Tülin; Süt, Necdet; Korkmaz, Selma; Usta, Ufuk; Öz Puyan, Fulya; Genç, Ezgi; Cezik, Mert; Binboğa Tutuğ, Busem; Köstek, Osman; Tozkir, Hilmi

2018-02-08

BRAF is the most common mutation in melanoma. The most common subtype is BRAF V600E, followed by V600K. Initially, the authors aimed to investigate whether clinicopathological features of melanoma are associated with BRAF mutations. We then aimed to present the relationships between the clinicopathological features and the mutated subtype (V600E vs V600K). 61 patients with metastatic malignant melanoma (affecting the lymph node or other distant sites) were selected. Patient data regarding age at the time of diagnosis, sex, metastatic site (lymph node, distant metastasis or both) and primary tumour site were obtained from the hospital's database. Tissue samples containing at least 30% tumour cells were isolated from the specimens of 61 patients (24 samples from primary tumours and 37 from metastatic foci) for BRAF analysis. Comparisons between the BRAF V600 mutation and clinicopathological and histopathological features were performed. BRAF V600 mutation was detected in 34 (55.7%) patients. The subtype was BRAF V600E in 22 (64.7%) patients, BRAF V600K in 11(32.4%) patients and BRAF V600R in 1(2.9%) patient. The crucial results of the present study may be summarized as follows: i) BRAF V600 mutation was more common in older patients and tumors with BRAF V600 mutation revealed necrosis and LVI more commonly than wild-type tumors, ii) BRAF V600K mutation was more common in older patients and BRAF V600K mutated tumors exhibited ulceration more commonly than tumors with BRAF V600E mutation (close to significant). The BRAF V600 mutation may have interactions with prognostic clinicoptahological features of melanoma including necrosis and lymphovascular invasion. V600K mutation may be more common than expected and may have different associations with properties of the tumor such as tumor ulceration and patient age. Investigation of the mutated subtype of the BRAF gene may therefore reveal more detailed data about the management of melanoma and may also prevent missing of

Interfacial microstructure in a B{sub 4}C/Al composite fabricated by pressureless infiltration.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Luo, Z.; Song, Y.; Zhang, S.

In this work, B{sub 4}C particulate-reinforced Al composite was fabricated by a pressureless infiltration technique, and its interfacial microstructure was studied in detail by X-ray diffraction as well as by scanning and transmission electron microscopy. The B{sub 4}C phase was unstable in Al melt during the infiltration process, forming AlB{sub 10}-type AlB{sub 24}C{sub 4} or Al{sub 2.1}B{sub 51}C{sub 8} as a major reactant phase. The Al matrix was large grains (over 10 {micro}m), which had no definite orientation relationships (ORs) with the randomly orientated B{sub 4}C or its reactant particles, except for possible nucleation sites with {l_brace}011{r_brace}{sub B{sub 4}C} almostmore » parallel to {l_brace}111{r_brace}{sub Al} at a deviation angle of 1.5 deg. Both B{sub 4}C-Al and reactant-Al interfaces are semicoherent and free of other phases. A comparison was made with the SiC/Al composite fabricated similarly by the pressureless infiltration. It was suggested that the lack of ORs between the Al matrix and reinforced particles, except for possible nucleation sites, is the common feature of the composites prepared by the infiltration method.« less

17 CFR 260.11b-4 - Definition of “cash transaction” in section 311(b)(4).

Code of Federal Regulations, 2011 CFR

2011-04-01

... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Definition of âcash transactionâ in section 311(b)(4). 260.11b-4 Section 260.11b-4 Commodity and Securities Exchanges SECURITIES... securities sold is made within 7 days after delivery of the goods or securities in currency or in checks or...

Simple theory for the dependence of the electrical resistance of the magnetic superconductors TmRh/sub 4/B/sub 4/ and ErRh/sub 4/B/sub 4/ on temperature and field

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meijer, H.C.; Andriessen, J.; Postma, H.

1986-04-01

A phenomenological description for the temperature and magnetic field dependence of the electrical resistance R of polycrystalline samples of the reentrant superconductors TmRh/sub 4/B/sub 4/ and ErRh/sub 4/B/sub 4/ is given on the basis of two assumptions: (1) Due to the anisotropic values of the rare-earth ions the critical field of the crystallites depends on the direction of the externally applied field, which leads to an increasing number of normal crystallites with increasing field. For the dependence of the magnetization M on temperature, a molecular field model is used. (2) The bulk resistance R of the sample depends in amore » linear way on the fraction of normal crystallites. There is a qualitative agreement with the experimental results of Hamaker et al. and of Ott et al. It is also shown that an applied field H/sub e/ is equal to the orbital critical field H(/sub c//sub 2/ for the temperature at which R(H/sub e/, T) starts deviating from the resistance of the normal sample.« less

12 CFR Appendix B to Subpart B of... - Article 4A, Funds Transfers

Code of Federal Regulations, 2013 CFR

2013-01-01

... 12 Banks and Banking 2 2013-01-01 2013-01-01 false Article 4A, Funds Transfers B Appendix B to...—Article 4A, Funds Transfers Part 1—Subject Matter and Definitions Section 4A-101. Short Title This Article... otherwise provided in section 4A-108, this Article applies to funds transfers defined in section 4A-104...

12 CFR Appendix B to Subpart B of... - Article 4A, Funds Transfers

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 2 2014-01-01 2014-01-01 false Article 4A, Funds Transfers B Appendix B to...—Article 4A, Funds Transfers Part 1—Subject Matter and Definitions Section 4A-101. Short Title This Article... otherwise provided in section 4A-108, this Article applies to funds transfers defined in section 4A-104...

12 CFR Appendix B to Subpart B of... - Article 4A, Funds Transfers

Code of Federal Regulations, 2012 CFR

2012-01-01

... 12 Banks and Banking 2 2012-01-01 2012-01-01 false Article 4A, Funds Transfers B Appendix B to...—Article 4A, Funds Transfers Part 1—Subject Matter and Definitions Section 4A-101. Short Title This Article... otherwise provided in section 4A-108, this Article applies to funds transfers defined in section 4A-104...

12 CFR Appendix B to Subpart B of... - Article 4A, Funds Transfers

Code of Federal Regulations, 2011 CFR

2011-01-01

... 12 Banks and Banking 2 2011-01-01 2011-01-01 false Article 4A, Funds Transfers B Appendix B to...—Article 4A, Funds Transfers Part 1—Subject Matter and Definitions Section 4A-101. Short Title This Article... otherwise provided in section 4A-108, this Article applies to funds transfers defined in section 4A-104...

12 CFR Appendix B to Subpart B of... - Article 4A, Funds Transfers

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 2 2010-01-01 2010-01-01 false Article 4A, Funds Transfers B Appendix B to...—Article 4A, Funds Transfers Part 1—Subject Matter and Definitions Section 4A-101. Short Title This Article... otherwise provided in section 4A-108, this Article applies to funds transfers defined in section 4A-104...

21 CFR 189.300 - Hydrogenated 4,4′-isopropyl-idene-diphenol-phosphite ester resins.

Code of Federal Regulations, 2014 CFR

2014-04-01

... manufacture of rigid vinyl chloride polymer bottles. (b) Food containing any added or detectable levels of... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Hydrogenated 4,4â²-isopropyl-idene-diphenol-phosphite ester resins. 189.300 Section 189.300 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF...

CD300b regulates the phagocytosis of apoptotic cells via phosphatidylserine recognition

PubMed Central

Murakami, Y; Tian, L; Voss, O H; Margulies, D H; Krzewski, K; Coligan, J E

2014-01-01

The CD300 receptor family members are a group of molecules that modulate a variety of immune cell processes. We show that mouse CD300b (CLM7/LMIR5), expressed on myeloid cells, recognizes outer membrane-exposed phosphatidylserine (PS) and does not, as previously reported, directly recognize TIM1 or TIM4. CD300b accumulates in phagocytic cups along with F-actin at apoptotic cell contacts, thereby facilitating their engulfment. The CD300b-mediated activation signal is conveyed through CD300b association with the adaptor molecule DAP12, and requires a functional DAP12 ITAM motif. Binding of apoptotic cells promotes the activation of the PI3K-Akt kinase pathway in macrophages, while silencing of CD300b expression diminishes PI3K-Akt kinase activation and impairs efferocytosis. Collectively, our data show that CD300b recognizes PS as a ligand, and regulates the phagocytosis of apoptotic cells via the DAP12 signaling pathway. PMID:25034781

Observation of e+e-→ϕ χc 1 and ϕ χc 2 at √{s }=4.600 GeV

NASA Astrophysics Data System (ADS)

Ablikim, M.; Achasov, M. N.; Ahmed, S.; Albrecht, M.; Amoroso, A.; An, F. F.; An, Q.; Bai, J. Z.; Bai, Y.; Bakina, O.; Baldini Ferroli, R.; Ban, Y.; Bennett, D. W.; Bennett, J. V.; Berger, N.; Bertani, M.; Bettoni, D.; Bian, J. M.; Bianchi, F.; Boger, E.; Boyko, I.; Briere, R. A.; Cai, H.; Cai, X.; Cakir, O.; Calcaterra, A.; Cao, G. F.; Cetin, S. A.; Chai, J.; Chang, J. F.; Chelkov, G.; Chen, G.; Chen, H. S.; Chen, J. C.; Chen, M. L.; Chen, P. L.; Chen, S. J.; Chen, X. R.; Chen, Y. B.; Chu, X. K.; Cibinetto, G.; Dai, H. L.; Dai, J. P.; Dbeyssi, A.; Dedovich, D.; Deng, Z. Y.; Denig, A.; Denysenko, I.; Destefanis, M.; de Mori, F.; Ding, Y.; Dong, C.; Dong, J.; Dong, L. Y.; Dong, M. Y.; Dou, Z. L.; Du, S. X.; Duan, P. F.; Fang, J.; Fang, S. S.; Fang, Y.; Farinelli, R.; Fava, L.; Fegan, S.; Feldbauer, F.; Felici, G.; Feng, C. Q.; Fioravanti, E.; Fritsch, M.; Fu, C. D.; Gao, Q.; Gao, X. L.; Gao, Y.; Gao, Y. G.; Gao, Z.; Garzia, I.; Goetzen, K.; Gong, L.; Gong, W. X.; Gradl, W.; Greco, M.; Gu, M. H.; Gu, Y. T.; Guo, A. Q.; Guo, R. P.; Guo, Y. P.; Haddadi, Z.; Han, S.; Hao, X. Q.; Harris, F. A.; He, K. L.; He, X. Q.; Heinsius, F. H.; Held, T.; Heng, Y. K.; Holtmann, T.; Hou, Z. L.; Hu, H. M.; Hu, T.; Hu, Y.; Huang, G. S.; Huang, J. S.; Huang, X. T.; Huang, X. Z.; Huang, Z. L.; Hussain, T.; Ikegami Andersson, W.; Ji, Q.; Ji, Q. P.; Ji, X. B.; Ji, X. L.; Jiang, X. S.; Jiang, X. Y.; Jiao, J. B.; Jiao, Z.; Jin, D. P.; Jin, S.; Jin, Y.; Johansson, T.; Julin, A.; Kalantar-Nayestanaki, N.; Kang, X. L.; Kang, X. S.; Kavatsyuk, M.; Ke, B. C.; Khan, T.; Khoukaz, A.; Kiese, P.; Kliemt, R.; Koch, L.; Kolcu, O. B.; Kopf, B.; Kornicer, M.; Kuemmel, M.; Kuessner, M.; Kuhlmann, M.; Kupsc, A.; Kühn, W.; Lange, J. S.; Lara, M.; Larin, P.; Lavezzi, L.; Leithoff, H.; Leng, C.; Li, C.; Li, Cheng; Li, D. M.; Li, F.; Li, F. Y.; Li, G.; Li, H. B.; Li, H. J.; Li, J. C.; Li, Jin; Li, K. J.; Li, Kang; Li, Ke; Li, Lei; Li, P. L.; Li, P. R.; Li, Q. Y.; Li, W. D.; Li, W. G.; Li, X. L.; Li, X. N.; Li, X. Q.; Li, Z. B.; Liang, H.; Liang, Y. F.; Liang, Y. T.; Liao, G. R.; Lin, D. X.; Liu, B.; Liu, B. J.; Liu, C. X.; Liu, D.; Liu, F. H.; Liu, Fang; Liu, Feng; Liu, H. B.; Liu, H. M.; Liu, Huanhuan; Liu, Huihui; Liu, J. B.; Liu, J. P.; Liu, J. Y.; Liu, K.; Liu, K. Y.; Liu, Ke; Liu, L. D.; Liu, P. L.; Liu, Q.; Liu, S. B.; Liu, X.; Liu, Y. B.; Liu, Z. A.; Liu, Zhiqing; Long, Y. F.; Lou, X. C.; Lu, H. J.; Lu, J. G.; Lu, Y.; Lu, Y. P.; Luo, C. L.; Luo, M. X.; Luo, X. L.; Lyu, X. R.; Ma, F. C.; Ma, H. L.; Ma, L. L.; Ma, M. M.; Ma, Q. M.; Ma, T.; Ma, X. N.; Ma, X. Y.; Ma, Y. M.; Maas, F. E.; Maggiora, M.; Malik, Q. A.; Mao, Y. J.; Mao, Z. P.; Marcello, S.; Meng, Z. X.; Messchendorp, J. G.; Mezzadri, G.; Min, J.; Min, T. J.; Mitchell, R. E.; Mo, X. H.; Mo, Y. J.; Morales Morales, C.; Muchnoi, N. Yu.; Muramatsu, H.; Musiol, P.; Mustafa, A.; Nefedov, Y.; Nerling, F.; Nikolaev, I. B.; Ning, Z.; Nisar, S.; Niu, S. L.; Niu, X. Y.; Olsen, S. L.; Ouyang, Q.; Pacetti, S.; Pan, Y.; Papenbrock, M.; Patteri, P.; Pelizaeus, M.; Pellegrino, J.; Peng, H. P.; Peters, K.; Pettersson, J.; Ping, J. L.; Ping, R. G.; Pitka, A.; Poling, R.; Prasad, V.; Qi, H. R.; Qi, M.; Qian, S.; Qiao, C. F.; Qin, N.; Qin, X. S.; Qin, Z. H.; Qiu, J. F.; Rashid, K. H.; Redmer, C. F.; Richter, M.; Ripka, M.; Rolo, M.; Rong, G.; Rosner, Ch.; Sarantsev, A.; Savrié, M.; Schnier, C.; Schoenning, K.; Shan, W.; Shao, M.; Shen, C. P.; Shen, P. X.; Shen, X. Y.; Sheng, H. Y.; Song, J. J.; Song, W. M.; Song, X. Y.; Sosio, S.; Sowa, C.; Spataro, S.; Sun, G. X.; Sun, J. F.; Sun, L.; Sun, S. S.; Sun, X. H.; Sun, Y. J.; Sun, Y. K.; Sun, Y. Z.; Sun, Z. J.; Sun, Z. T.; Tang, C. J.; Tang, G. Y.; Tang, X.; Tapan, I.; Tiemens, M.; Tsednee, B.; Uman, I.; Varner, G. S.; Wang, B.; Wang, B. L.; Wang, D.; Wang, D. Y.; Wang, Dan; Wang, K.; Wang, L. L.; Wang, L. S.; Wang, M.; Wang, Meng; Wang, P.; Wang, P. L.; Wang, W. P.; Wang, X. F.; Wang, Y.; Wang, Y. D.; Wang, Y. F.; Wang, Y. Q.; Wang, Z.; Wang, Z. G.; Wang, Z. Y.; Wang, Zongyuan; Weber, T.; Wei, D. H.; Weidenkaff, P.; Wen, S. P.; Wiedner, U.; Wolke, M.; Wu, L. H.; Wu, L. J.; Wu, Z.; Xia, L.; Xia, Y.; Xiao, D.; Xiao, H.; Xiao, Y. J.; Xiao, Z. J.; Xie, Y. G.; Xie, Y. H.; Xiong, X. A.; Xiu, Q. L.; Xu, G. F.; Xu, J. J.; Xu, L.; Xu, Q. J.; Xu, Q. N.; Xu, X. P.; Yan, L.; Yan, W. B.; Yan, W. C.; Yan, Y. H.; Yang, H. J.; Yang, H. X.; Yang, L.; Yang, Y. H.; Yang, Y. X.; Ye, M.; Ye, M. H.; Yin, J. H.; You, Z. Y.; Yu, B. X.; Yu, C. X.; Yu, J. S.; Yuan, C. Z.; Yuan, Y.; Yuncu, A.; Zafar, A. A.; Zeng, Y.; Zeng, Z.; Zhang, B. X.; Zhang, B. Y.; Zhang, C. C.; Zhang, D. H.; Zhang, H. H.; Zhang, H. Y.; Zhang, J.; Zhang, J. L.; Zhang, J. Q.; Zhang, J. W.; Zhang, J. Y.; Zhang, J. Z.; Zhang, K.; Zhang, L.; Zhang, S. Q.; Zhang, X. Y.; Zhang, Y. H.; Zhang, Y. T.; Zhang, Yang; Zhang, Yao; Zhang, Yu; Zhang, Z. H.; Zhang, Z. P.; Zhang, Z. Y.; Zhao, G.; Zhao, J. W.; Zhao, J. Y.; Zhao, J. Z.; Zhao, Lei; Zhao, Ling; Zhao, M. G.; Zhao, Q.; Zhao, S. J.; Zhao, T. C.; Zhao, Y. B.; Zhao, Z. G.; Zhemchugov, A.; Zheng, B.; Zheng, J. P.; Zheng, W. J.; Zheng, Y. H.; Zhong, B.; Zhou, L.; Zhou, X.; Zhou, X. K.; Zhou, X. R.; Zhou, X. Y.; Zhou, Y. X.; Zhu, J.; Zhu, J.; Zhu, K.; Zhu, K. J.; Zhu, S.; Zhu, S. H.; Zhu, X. L.; Zhu, Y. C.; Zhu, Y. S.; Zhu, Z. A.; Zhuang, J.; Zou, B. S.; Zou, J. H.; Besiii Collaboration

2018-02-01

Using a data sample collected with the BESIII detector operating at the BEPCII storage ring at a center-of-mass energy of √{s }=4.600 GeV , we search for the production of e+e-→ϕ χc 0 ,1 ,2 . A search is also performed for the charmonium-like state X (4140 ) in the radiative transition e+e-→γ X (4140 ) with X (4140 ) subsequently decaying into ϕ J /ψ . The processes e+e-→ϕ χc 1 and ϕ χc 2 are observed for the first time, each with a statistical significance of more than 10 σ , and the Born cross sections are measured to be (4. 2-1.0+1.7±0.3 ) and (6. 7-1.7+3.4±0.5 ) pb , respectively, where the first uncertainties are statistical and the second systematic. No significant signals are observed for e+e-→ϕ χc 0 and e+e-→γ X (4140 ) and upper limits on the Born cross sections at 90% C.L. are provided at √{s }=4.600 GeV .

46 CFR 177.300 - Structural design.

Code of Federal Regulations, 2010 CFR

2010-10-01

...) CONSTRUCTION AND ARRANGEMENT Hull Structure § 177.300 Structural design. Except as otherwise allowed by this... (incorporated by reference, see 46 CFR 175.600); (b) Steel hull vessels: (1) Lloyd's Yachts and Small Craft; or (2) ABS Steel Vessel Rules (< 61 Meters)(incorporated by reference, see 46 CFR 175.600); (c) Fiber...

46 CFR 177.300 - Structural design.

Code of Federal Regulations, 2014 CFR

2014-10-01

...) CONSTRUCTION AND ARRANGEMENT Hull Structure § 177.300 Structural design. Except as otherwise allowed by this... (incorporated by reference, see 46 CFR 175.600); (b) Steel hull vessels: (1) Lloyd's Yachts and Small Craft; or (2) ABS Steel Vessel Rules (<61 Meters)(incorporated by reference, see 46 CFR 175.600); (c) Fiber...

46 CFR 177.300 - Structural design.

Code of Federal Regulations, 2013 CFR

2013-10-01

...) CONSTRUCTION AND ARRANGEMENT Hull Structure § 177.300 Structural design. Except as otherwise allowed by this... (incorporated by reference, see 46 CFR 175.600); (b) Steel hull vessels: (1) Lloyd's Yachts and Small Craft; or (2) ABS Steel Vessel Rules (<61 Meters)(incorporated by reference, see 46 CFR 175.600); (c) Fiber...

46 CFR 177.300 - Structural design.

Code of Federal Regulations, 2011 CFR

2011-10-01

...) CONSTRUCTION AND ARRANGEMENT Hull Structure § 177.300 Structural design. Except as otherwise allowed by this... (incorporated by reference, see 46 CFR 175.600); (b) Steel hull vessels: (1) Lloyd's Yachts and Small Craft; or (2) ABS Steel Vessel Rules (< 61 Meters)(incorporated by reference, see 46 CFR 175.600); (c) Fiber...

46 CFR 177.300 - Structural design.

Code of Federal Regulations, 2012 CFR

2012-10-01

...) CONSTRUCTION AND ARRANGEMENT Hull Structure § 177.300 Structural design. Except as otherwise allowed by this... (incorporated by reference, see 46 CFR 175.600); (b) Steel hull vessels: (1) Lloyd's Yachts and Small Craft; or (2) ABS Steel Vessel Rules (< 61 Meters)(incorporated by reference, see 46 CFR 175.600); (c) Fiber...

[Cytogenetical comparison of restorers TP-4 and D minghui63 and maintainer D46B of autotetraploid rice].

PubMed

Long, Wen-Bo; Luan, Li; Wang, Xing; Liu, Yu-Hua; Tu, Sheng-Bin; Kong, Fan-Lun; He, Tao

2007-04-01

Cytogenetical comparison was made between high seed set restorers TP-4 and D minghui63 and eminent maintainer line D46B of autotetraploid rice. The meiosis observation demonstrated the genomes of our autotetraploid materials were all 2n = 48, the same as those in mitosis observation. Low percentages of univalent and trivalent in metaphase I (MI) of restorers TP-4 and D minghui63 and in metaphase I (MI) of maintainer line D46B of autotetraploid rice were observed. And the percentages of chromosome pairing were all over 99%, showing eminent cytological character. The frequency of TP-4 and D minghui63 in metaphase I (MI) was 2.00/PMC and 2.26/PMC, respectively. However the frequency of D46B was 6.00/PMC, significantly higher than those of TP-4 and D minghui63. It indicated that the maintainer D46B has better chromosome pairing capability in metaphase I (MI). While, the frequency of lagging chromosomes of the maintainer D46B in anaphase I (AI) was 10.62%, significantly lower than that of TP-4 (19.44%) or D minghui63 (23.14%), and it was close to the level of diploid control (7.30%). In telophase I (TI), maintainer D46B exhibited a lower frequency of microkernel, and in telophase II (TII) the frequency of normal quartered microspore of maintainer D46B was not only higher than that of TP-4 or D minghui63 but also than that of diploid control. The percentage of the cell observed chromosome lagging in A1 and the percentage of abnormal cell in TI showed a greatly significant positive correlation. That may demonstrate chromo some separation in anaphase I (AI) and microkernel formation in telophase I (TI) are controlled by the same dominant single gene or the major gene of QTL.

40 CFR 600.507-08 - Running change data requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 31 2012-07-01 2012-07-01 false Running change data requirements. 600... Running change data requirements. (a) Except as specified in paragraph (d) of this section, the manufacturer shall submit additional running change fuel economy data as specified in paragraph (b) of this...

40 CFR 600.507-08 - Running change data requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... POLICY FUEL ECONOMY AND GREENHOUSE GAS EXHAUST EMISSIONS OF MOTOR VEHICLES Procedures for Determining Manufacturer's Average Fuel Economy and Manufacturer's Average Carbon-Related Exhaust Emissions § 600.507-08... manufacturer shall submit additional running change fuel economy data as specified in paragraph (b) of this...

21 CFR 189.300 - Hydrogenated 4,4′-isopropyl-idene-diphenol-phosphite ester resins.

Code of Federal Regulations, 2012 CFR

2012-04-01

... resins are used in the manufacture of rigid vinyl chloride polymer bottles. (b) Food containing any added... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Hydrogenated 4,4â²-isopropyl-idene-diphenol-phosphite ester resins. 189.300 Section 189.300 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF...

21 CFR 189.300 - Hydrogenated 4,4′-isopropyl-idene-diphenol-phosphite ester resins.

Code of Federal Regulations, 2013 CFR

2013-04-01

... resins are used in the manufacture of rigid vinyl chloride polymer bottles. (b) Food containing any added... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Hydrogenated 4,4â²-isopropyl-idene-diphenol-phosphite ester resins. 189.300 Section 189.300 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF...

Revisiting the biology of infant t(4;11)/MLL-AF4+ B-cell acute lymphoblastic leukemia

PubMed Central

Bueno, Clara; Prieto, Cristina; Acha, Pamela; Stam, Ronald W.; Marschalek, Rolf; Menéndez, Pablo

2015-01-01

Infant B-cell acute lymphoblastic leukemia (B-ALL) accounts for 10% of childhood ALL. The genetic hallmark of most infant B-ALL is chromosomal rearrangements of the mixed-lineage leukemia (MLL) gene. Despite improvement in the clinical management and survival (∼85-90%) of childhood B-ALL, the outcome of infants with MLL-rearranged (MLL-r) B-ALL remains dismal, with overall survival <35%. Among MLL-r infant B-ALL, t(4;11)+ patients harboring the fusion MLL-AF4 (MA4) display a particularly poor prognosis and a pro-B/mixed phenotype. Studies in monozygotic twins and archived blood spots have provided compelling evidence of a single cell of prenatal origin as the target for MA4 fusion, explaining the brief leukemia latency. Despite its aggressiveness and short latency, current progress on its etiology, pathogenesis, and cellular origin is limited as evidenced by the lack of mouse/human models recapitulating the disease phenotype/latency. We propose this is because infant cancer is from an etiologic and pathogenesis standpoint distinct from adult cancer and should be seen as a developmental disease. This is supported by whole-genome sequencing studies suggesting that opposite to the view of cancer as a “multiple-and-sequential-hit” model, t(4;11) alone might be sufficient to spawn leukemia. The stable genome of these patients suggests that, in infant developmental cancer, one “big-hit” might be sufficient for overt disease and supports a key contribution of epigenetics and a prenatal cell of origin during a critical developmental window of stem cell vulnerability in the leukemia pathogenesis. Here, we revisit the biology of t(4;11)+ infant B-ALL with an emphasis on its origin, genetics, and disease models. PMID:26463423

Oxide Morphology of a FeCrAl Alloy, Kanthal APMT, following Extended Aging at 300-600C

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Nan; Parker, Stephen Scott; Wood, Elizabeth Sooby

Iron-chromium-aluminum (FeCrAl) alloys are of interest to the nuclear materials community due to their resistance to high temperature steam oxidation under accident conditions. The present work investigates oxide formation at temperatures relevant to light water reactor cladding operation following extended aging to assess growth kinetics, chemical composition, and microstructure of oxide formation on a commercial FeCrAl alloy, Fe-21wt.%Cr-5wt.%Al-3wt.%Mo (Kanthal APMT). Aging treatments were performed for 100-1000 hours in stagnant air at 300, 400, 500, and 600 °C, respectively. Oxide growth behavior under the investigated conditions follows a logarithmic time dependence. When the oxidization temperature is 400 °C or below, themore » oxide is amorphous. At 500 °C, isolated crystalline regions start to appear during short period aging time and expand with extended exposures. Crystalline α-Al2O3 oxide film develops at 600 °C and the correlated logarithmic rate constant decreases significantly, indicating enhanced oxidation resistance of the formed oxide film. In addition, Mo segregation at grain boundaries has been observed when the aging temperature exceeds 500 °C. The results of this study can be viewed as an upper bounding result for potential oxide coarsening during reactor operation.« less

Polarization Observations of Giant Radio Pulses from the Millisecond Pulsar B1937+21 at a Frequency of 600 MHz

NASA Astrophysics Data System (ADS)

Popov, M. V.; Soglasnov, V. A.; Kondrat'ev, V. I.; Kostyuk, S. V.

2004-02-01

We performed polarization observations of giant radio pulses from the millisecond pulsar B1937+21. The observations were carried out in July 2002 with the 64-m Kalyazin radio telescope at a frequency of 600 MHz in two polarization channels with left- and right-hand circular polarizations (RCP and LCP). We used the S2 data acquisition system with a time resolution of 125 ns. The duration of an observing session was 20 min. We detected twelve giant radio pulses with peak flux densities higher than 1000 Jy; five and seven of these pulses appeared in the RCP and LCP channels, respectively. We found no event that exceeded the established detection threshold simultaneously in the two polarization channels. Thus, we may conclude that the detected giant pulses have a high degree of circular polarization, with the frequency of occurrence of RCP and LCP pulses being the same.

EphB4 promotes or suppresses Ras/MEK/ERK pathway in a context-dependent manner: Implications for EphB4 as a cancer target.

PubMed

Xiao, Zhan; Carrasco, Rosa; Kinneer, Krista; Sabol, Darrin; Jallal, Bahija; Coats, Steve; Tice, David A

2012-06-01

EphB4 is a member of the Eph receptor tyrosine kinase family shown to act in neuronal guidance and mediate venal/arterial separation. In contrast to these more established roles, EphB4's function in cancer is much less clear. Here we illustrate both tumor promoting as well as suppressing roles of EphB4, by showing that its activation resulted in inhibition of the Ras/ERK pathway in endothelial cells but activation of the same pathway in MCF-7 breast cancer cells. This was true if EphB4 was stimulated with EphrinB2, its natural ligand, or an agonistic monoclonal antibody for EphB4. Correspondingly, EphB4 activation stimulated MCF7 growth while inhibiting HUVEC cell proliferation. The reason for these dramatic differences is due to functional coupling of EphB4 to different downstream effectors. Reduction of p120 RasGAP in HUVEC cells attenuated the inhibitory effect of EphB4 activation on the ERK pathway, whereas knockdown of PP2A in MCF7 cells attenuated EphB4 activation of the ERK pathway. This represents the first time a functional coupling between Eph receptor and PP2A has been demonstrated leading to activation of an oncogenic pathway. Our study illustrates the caveats and potential challenges of targeting EphB4 for cancer therapy due to the conflicting effects on cancer cell and endothelial cell compartments.

50 CFR 600.227 - Lobbying.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Lobbying. 600.227 Section 600.227 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MAGNUSON-STEVENS ACT PROVISIONS Council Membership § 600.227 Lobbying. (a) Council...

50 CFR 600.227 - Lobbying.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 50 Wildlife and Fisheries 12 2013-10-01 2013-10-01 false Lobbying. 600.227 Section 600.227 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MAGNUSON-STEVENS ACT PROVISIONS Council Membership § 600.227 Lobbying. (a) Council...

44 CFR 17.600 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Purpose. 17.600 Section 17.600 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY GENERAL GOVERNMENTWIDE REQUIREMENTS FOR DRUG-FREE WORKPLACE (GRANTS) § 17.600 Purpose. (a) The...

Acousto-optic interaction in alpha-BaB(2)O(4)and Li(2)B(4)O(7) crystals.

PubMed

Martynyuk-Lototska, Irina; Mys, Oksana; Dudok, Taras; Adamiv, Volodymyr; Smirnov, Yevgen; Vlokh, Rostyslav

2008-07-01

Experimental studies and analysis of acousto-optic diffraction in alpha-BaB(2)O(4) and Li(2)B(4)O(7) crystals are given. Ultrasonic wave velocity, elastic compliance and stiffness coefficients, and piezo-optic and photoelastic coefficients of alpha-BaB(2)O(4) and Li(2)B(4)O(7) crystals are determined. The acousto-optic figure of merit has been estimated for different possible geometries of acousto-optic interaction. It is shown that the acousto-optic figures of merit for alpha-BaB(2)O(4) crystals reach the value M(2)=(270 +/- 70) x 10(-15) s(3)/kg for the case of interaction with the slowest ultrasonic wave. The directions of propagation and polarization of those acoustic waves are obtained on the basis of construction of acoustic slowness surfaces. The acousto-optic diffraction is experimentally studied for alpha-BaB(2)O(4) and Li(2)B(4)O(7) crystals.

50 CFR 600.505 - Prohibitions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 8 2010-10-01 2010-10-01 false Prohibitions. 600.505 Section 600.505 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MAGNUSON-STEVENS ACT PROVISIONS Foreign Fishing § 600.505 Prohibitions. (a) It is...

Sevoflurane represses the self-renewal ability by regulating miR-7a,7b/Klf4 signalling pathway in mouse embryonic stem cells.

PubMed

Wang, Qimin; Li, Guifeng; Li, Baolin; Chen, Qiu; Lv, Dongdong; Liu, Jiaying; Ma, Jieyu; Sun, Nai; Yang, Longqiu; Fei, Xuejie; Song, Qiong

2016-10-01

Sevoflurane is a frequently-used clinical inhalational anaesthetic and can cause toxicity to embryos during foetal development. Embryonic stem cells (ESCs) are derived from the inner cell mass of blastospheres and can be used as a useful model of early development. Here, we found that sevoflurane significantly influenced self-renewal ability of mESCs on stemness maintenance and cell proliferation. The cell cycle was arrested via G1 phase delay. We further found that sevoflurane upregulated expression of miR-7a,7b to repress self-renewal. Next we performed rescue experiments and found that after adding miR-7a,7b inhibitor into mESCs treated with sevoflurane, its influence on self-renewal could be blocked. Further we identified stemness factor Klf4 as the direct target of miR-7a,7b. Overexpression of Klf4 restored self-renewal ability repressed by miR-7a,7b or sevoflurane. In this work, we determined that sevoflurane repressed self-renewal ability by regulating the miR-7a,7b/Klf4 signalling pathway in mESCs. Our study demonstrated molecular mechanism underlying the side effects of sevoflurane during early development, laying the foundation for studies on safe usage of inhalational anaesthetic during non-obstetric surgery. © 2016 John Wiley & Sons Ltd.

21 CFR 600.90 - Waivers.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Waivers. 600.90 Section 600.90 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) BIOLOGICS BIOLOGICAL PRODUCTS: GENERAL Reporting of Adverse Experiences § 600.90 Waivers. (a) A licensed manufacturer may ask...

24 CFR 598.600 - Applicability.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 3 2012-04-01 2012-04-01 false Applicability. 598.600 Section 598.600 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... Grants § 598.600 Applicability. This subpart applies to a project or activity proposed by an Empowerment...

24 CFR 598.600 - Applicability.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 3 2014-04-01 2013-04-01 true Applicability. 598.600 Section 598.600 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... Grants § 598.600 Applicability. This subpart applies to a project or activity proposed by an Empowerment...

24 CFR 598.600 - Applicability.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 3 2013-04-01 2013-04-01 false Applicability. 598.600 Section 598.600 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... Grants § 598.600 Applicability. This subpart applies to a project or activity proposed by an Empowerment...

28 CFR 600.5 - Staff.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Staff. 600.5 Section 600.5 Judicial Administration OFFICES OF INDEPENDENT COUNSEL, DEPARTMENT OF JUSTICE GENERAL POWERS OF SPECIAL COUNSEL § 600.5 Staff. A Special Counsel may request the assignment of appropriate Department employees to assist the...

28 CFR 600.5 - Staff.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Staff. 600.5 Section 600.5 Judicial Administration OFFICES OF INDEPENDENT COUNSEL, DEPARTMENT OF JUSTICE GENERAL POWERS OF SPECIAL COUNSEL § 600.5 Staff. A Special Counsel may request the assignment of appropriate Department employees to assist the...

28 CFR 600.5 - Staff.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Staff. 600.5 Section 600.5 Judicial Administration OFFICES OF INDEPENDENT COUNSEL, DEPARTMENT OF JUSTICE GENERAL POWERS OF SPECIAL COUNSEL § 600.5 Staff. A Special Counsel may request the assignment of appropriate Department employees to assist the...

28 CFR 600.5 - Staff.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Staff. 600.5 Section 600.5 Judicial Administration OFFICES OF INDEPENDENT COUNSEL, DEPARTMENT OF JUSTICE GENERAL POWERS OF SPECIAL COUNSEL § 600.5 Staff. A Special Counsel may request the assignment of appropriate Department employees to assist the...

28 CFR 600.5 - Staff.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Staff. 600.5 Section 600.5 Judicial Administration OFFICES OF INDEPENDENT COUNSEL, DEPARTMENT OF JUSTICE GENERAL POWERS OF SPECIAL COUNSEL § 600.5 Staff. A Special Counsel may request the assignment of appropriate Department employees to assist the...

RAF inhibitor resistance is mediated by dimerization of aberrantly spliced BRAF(V600E)

PubMed Central

Poulikakos, Poulikos I.; Persaud, Yogindra; Janakiraman, Manickam; Kong, Xiangju; Ng, Charles; Moriceau, Gatien; Shi, Hubing; Atefi, Mohammad; Titz, Bjoern; Gabay, May Tal; Salton, Maayan; Dahlman, Kimberly B.; Tadi, Madhavi; Wargo, Jennifer A.; Flaherty, Keith T.; Kelley, Mark C.; Misteli, Tom; Chapman, Paul B.; Sosman, Jeffrey A.; Graeber, Thomas G.; Ribas, Antoni; Lo, Roger S.; Rosen, Neal; Solit, David B.

2011-01-01

Summary Activated RAS promotes dimerization of members of the RAF kinase family1-3. ATP-competitive RAF inhibitors activate ERK signaling4-7 by transactivating RAF dimers4. In melanomas with mutant BRAF(V600E), levels of RAS activation are low and these drugs bind to BRAF(V600E) monomers and inhibit their activity. This tumor-specific inhibition of ERK signaling results in a broad therapeutic index and RAF inhibitors have remarkable clinical activity in patients with melanomas that harbor mutant BRAF(V600E)8. However, resistance invariably develops. Here, we identify a novel resistance mechanism. We find that a subset of cells resistant to vemurafenib (PLX4032, RG7204) express a 61kd variant form of BRAF(V600E) that lacks exons 4-8, a region that encompasses the RAS-binding domain. p61BRAF(V600E) exhibits enhanced dimerization in cells with low levels of RAS activation, as compared to full length BRAF(V600E). In cells in which p61BRAF(V600E) is expressed endogenously or ectopically, ERK signaling is resistant to the RAF inhibitor. Moreover, a mutation that abolishes the dimerization of p61BRAF(V600E) restores its sensitivity to vemurafenib. Finally, we identified BRAF(V600E) splicing variants lacking the RAS-binding domain in the tumors of six of 19 patients with acquired resistance to vemurafenib. These data support the model that inhibition of ERK signaling by RAF inhibitors is dependent on levels of RAS-GTP too low to support RAF dimerization and identify a novel mechanism of acquired resistance in patients: expression of splicing isoforms of BRAF(V600E) that dimerize in a RAS-independent manner. PMID:22113612

Development of an ErbB4 monoclonal antibody that blocks neuregulin-1-induced ErbB4 activation in cancer cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Okazaki, Shogo; Nakatani, Fumi; Masuko, Kazue

2016-01-29

The use of monoclonal antibodies (mAbs) for cancer therapy is one of the most important strategies for current cancer treatment. The epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases, which regulates cancer cell proliferation, survival, and migration, is a major molecular target for antibody-based therapy. ErbB4/HER4, which contains a ligand-binding extracellular region, is activated by several ligands, including neuregulins (NRGs), heparin-binding EGF-like growth factor, betacellulin and epiregulin. Although there are clinically approved antibodies for ErbB1 and ErbB2, there are no available therapeutic mAbs for ErbB4, and it is not known whether ErbB4 is a useful target for antibody-basedmore » cancer therapy. In this study, we developed an anti-ErbB4 mAb (clone P6-1) that suppresses NRG-dependent activation of ErbB4 and examined its effect on breast cancer cell proliferation in the extracellular matrix. - Highlights: • We newly generated four clones of human ErbB4 specific mAb. • ErbB4 mAb clone P6-1 blocks ErbB4 phosphorylation induced by NRG-1. • ErbB4 mAb clone P6-1 suppresses NRG-1-promoted breast cancer cells proliferation on three dimensional culture condition.« less

21 CFR 600.12 - Records.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 7 2013-04-01 2013-04-01 false Records. 600.12 Section 600.12 Food and Drugs FOOD... PRODUCTS: GENERAL Establishment Standards § 600.12 Records. (a) Maintenance of records. Records shall be... be traced by an inspector. Such records shall be legible and indelible, shall identify the person...

21 CFR 600.12 - Records.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 7 2012-04-01 2012-04-01 false Records. 600.12 Section 600.12 Food and Drugs FOOD... PRODUCTS: GENERAL Establishment Standards § 600.12 Records. (a) Maintenance of records. Records shall be... be traced by an inspector. Such records shall be legible and indelible, shall identify the person...

21 CFR 600.12 - Records.

Code of Federal Regulations, 2010 CFR

2010-04-01

... product upon notification by the Director, Center for Biologics Evaluation and Research or the Director... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Records. 600.12 Section 600.12 Food and Drugs FOOD... PRODUCTS: GENERAL Establishment Standards § 600.12 Records. (a) Maintenance of records. Records shall be...

21 CFR 600.12 - Records.

Code of Federal Regulations, 2011 CFR

2011-04-01

... product upon notification by the Director, Center for Biologics Evaluation and Research or the Director... 21 Food and Drugs 7 2011-04-01 2010-04-01 true Records. 600.12 Section 600.12 Food and Drugs FOOD... PRODUCTS: GENERAL Establishment Standards § 600.12 Records. (a) Maintenance of records. Records shall be...

40 CFR 600.011-93 - Reference materials.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Standard Test Method for Heat of Combustion of Liquid Hydrocarbon Fuels by Bomb Calorimeter 600.113-93, 600... Hydrocarbon Fuels by Bomb Calorimeter, IBR approved for §§ 600.113-93, 600.510-93, 600.113-08, and 600.510-08...