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Sample records for a4-a parkersburg wv

  1. 75 FR 26793 - Fypon, Ltd., Parkersburg, WV, Including Workers Whose Unemployment Insurance, (UI) Wages Are Paid...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-12

    ...] Fypon, Ltd., Parkersburg, WV, Including Workers Whose Unemployment Insurance, (UI) Wages Are Paid..., Including Workers Whose Unemployment Insurance, (UI) Wages Are Paid Through Therma-Tru Doors, Archbold, OH... reported under a separate unemployment insurance (UI) tax account under the name Therma-Tru...

  2. 2. VIEW EAST, REAR. see also WV2371, WV2372, WV2373, WV23710, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW EAST, REAR. see also WV-237-1, WV-237-2, WV-237-3, WV-237-10, WV-237-F-2, WV-237-G-3 and historic photographs WV-237-11, WV-237-12, WV-237-13, WV-237-14, WV-237-18, WV-237-F-5) - Parsons Nursery, Original Wash House, South side of U.S. Route 219, Parsons, Tucker County, WV

  3. 40 CFR 81.70 - Parkersburg (West Virginia)-Marietta (Ohio) Interstate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... (Ohio) Interstate Air Quality Control Region. 81.70 Section 81.70 Protection of Environment... (Ohio) Interstate Air Quality Control Region. The Parkersburg (West Virginia)-Marietta (Ohio) Interstate... County, Tyler County, Wetzel County, Wood County. In the State of Ohio: Athens County, Meigs...

  4. 40 CFR 81.70 - Parkersburg (West Virginia)-Marietta (Ohio) Interstate Air Quality Control Region.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (Ohio) Interstate Air Quality Control Region. 81.70 Section 81.70 Protection of Environment... (Ohio) Interstate Air Quality Control Region. The Parkersburg (West Virginia)-Marietta (Ohio) Interstate... County, Tyler County, Wetzel County, Wood County. In the State of Ohio: Athens County, Meigs...

  5. Greening America's Capitals - Charleston, WV

    EPA Pesticide Factsheets

    This Greening America’s Capitals report outlines design concepts that could improve Slack Plaza in Charleston, WV, by creating more opportunities for local businesses, better managing stormwater runoff, and increasing green space.

  6. 76 FR 80230 - Amendment of Class E Airspace; Huntington, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-23

    ... 14 CFR Part 71 [Docket No. FAA-2011-1057; Airspace Docket No. 11-AEA-21] Amendment of Class E... amend Class E airspace at Huntington, WV (76 FR 64295) Docket No. FAA-2011-1057. Interested parties...

  7. RadNet Air Data From Charleston, WV

    EPA Pesticide Factsheets

    This page presents radiation air monitoring and air filter analysis data for Charleston, WV from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

  8. 76 FR 28312 - Safety Zones; Fireworks Display Kanawha River, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-17

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zones; Fireworks Display Kanawha River, WV AGENCY... protect persons and vessels from the potential safety hazards associated with annual firework displays... movement of vessels within the established fireworks display areas. Entry into, transit through,...

  9. 76 FR 71936 - Upper Deckers Creek Watershed, Preston County, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-21

    ... Natural Resources Conservation Service Upper Deckers Creek Watershed, Preston County, WV AGENCY: Natural... Environmental Quality Guidelines (40 CFR part 1500); and the Natural Resources Conservation Service Guidelines (7 CFR part 650); the Natural Resources Conservation Service, U. S. Department of Agriculture,...

  10. METEOSAT IR and WV channels Fundamental Climate Data Record

    NASA Astrophysics Data System (ADS)

    Roebeling, Rob; John, Viju; Hewison, Tim; Lattanzio, Alessio; Schulz, Joerg

    2015-04-01

    The detection of climate change and analysis of climate variability at inter-annual scales requires long-term, well calibrated observations that are homogenised in time and space. Observations from EUMETSAT's series of Meteosat First Generation (MFG) and Meteosat Second Generation (MSG) geostationary satellites span a period from 1982 to today. Although these satellites provide data for climate analysis at multi-decadal scales, their applicability for such analysis is hampered by heterogeneities in the time series due to successive radiometers having different filter functions and changes in the calibration methodology. EUMETSAT initiated the activity to improve the quality of these data, and generates a Fundamental Climate Data Record (FCDR) of Water Vapour (WV) and Infrared (IR) channel radiances, i.e., a long-term data record of calibrated and quality-controlled sensor data designed to allow the generation of homogeneous products that are accurate and stable enough for climate monitoring. The generation of this FCDR is part of EUMETSATs activities in the European Re-Analysis of global CLIMate observations 2 (ERA-CLIM2) project. We present a method to inter-calibrate the complete time series of WV (6.3 µm) and IR (11.8 µm) channel radiances from MFG-MVIRI and MSG-SEVIRI observations. Our method is based on the principles of the Global Space-based Inter-Calibration System (GSICS). A systematic review of spectral conversion functions, which often dominate the errors, indicates that spectral changes of the WV channel from HIRS/2 to HIRS/3 triples the uncertainty of inter-calibrated METEOSAT WV radiances. We will show that these issues can be circumvented by using HIRS/2, AIRS, and IASI as reference instruments, and thus keeping the uncertainties due to spectral conversion similar throughout the time series. Finally we will present an evaluation of 30 years of recalibrated HIRS, MVIRI and /SEVIRI radiances from the IR and WV channels, and demonstrate their improved

  11. 75 FR 65251 - Proposed Amendment of Class E Airspace; Charleston, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-22

    ... examined during normal business hours at the office of the Eastern Service Center, Federal Aviation... more above the surface of the earth. * * * * * AEA WV E5 Charleston, WV Yeager Airport, Charleston,...

  12. 33 CFR 165.822 - Safety Zone; Fireworks Display, Kanawha River, WV.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Safety Zone; Fireworks Display... Safety Zone; Fireworks Display, Kanawha River, WV. (a) Safety zones. The following areas are designated... Virginia Special Olympics Fireworks Display Between mile 57.9 and 58.9, Charlestown, WV First Friday...

  13. 33 CFR 165.822 - Safety Zone; Fireworks Display, Kanawha River, WV.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Safety Zone; Fireworks Display... Safety Zone; Fireworks Display, Kanawha River, WV. (a) Safety zones. The following areas are designated... Virginia Special Olympics Fireworks Display Between mile 57.9 and 58.9, Charlestown, WV First Friday...

  14. 33 CFR 165.822 - Safety Zone; Fireworks Display, Kanawha River, WV.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Safety Zone; Fireworks Display... Safety Zone; Fireworks Display, Kanawha River, WV. (a) Safety zones. The following areas are designated... Virginia Special Olympics Fireworks Display Between mile 57.9 and 58.9, Charlestown, WV First Friday...

  15. 33 CFR 165.822 - Safety Zone; Fireworks Display, Kanawha River, WV.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Safety Zone; Fireworks Display... Safety Zone; Fireworks Display, Kanawha River, WV. (a) Safety zones. The following areas are designated... Virginia Special Olympics Fireworks Display Between mile 57.9 and 58.9, Charlestown, WV First Friday...

  16. 75 FR 55612 - TRG Insurance Solutions, LLC; Beckley, WV; Notice of Affirmative Determination Regarding...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration TRG Insurance Solutions, LLC; Beckley, WV; Notice of Affirmative... Solutions, LLC, Beckley, West Virginia (subject firm). The negative determination was issued on July...

  17. 76 FR 7584 - Matthews International Corporation, Bronze Division, Kingwood, WV; Notice of Affirmative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-10

    ... Employment and Training Administration Matthews International Corporation, Bronze Division, Kingwood, WV... workers and former workers of Matthews International Corporation, Bronze Division, Kingwood, West Virginia... activities related to the production of bronze burial markers and memorial products. In the request...

  18. Efficacy of a topical application of Certifect® (fipronil 6.26% w/v, amitraz 7.48% w/v, (S)-methoprene 5.63% w/v) for the treatment of canine generalized demodicosis

    PubMed Central

    Fourie, Josephus; Dumont, Pascal; Halos, Lénaïg; Beugnet, Frederic; Pollmeier, Matthias

    2013-01-01

    The efficacy of the treatment with Certifect ® (containing fipronil 6.26% w/v, amitraz 7.48% w/v, (S)-methoprene 5.63% w/v) applied topically was assessed in 18 dogs diagnosed with clinical generalized demodicosis. Three treatment regimens were compared over a 3-month period. Starting at Day 0, dogs were treated monthly (group 1) or every two weeks (group 2) with the combination of fipronil, amitraz, and (S)-methoprene or with monthly topical applications of the combination of amitraz and metaflumizone (group 3, reference treatment). Clinical examinations including deep skin scrapings were performed every month in order to evaluate the resolution of clinical signs and the reduction in mite counts. On Day 84, the percentage reduction of mite counts in group 1 was 99.8%, whereas no Demodex canis could be detected in groups 2 and 3 (i.e. 100% parasitological efficacy). As a result of the Demodex mite count reduction, the skin condition of the dogs improved significantly in all groups. This study illustrates, that both monthly and bi-weekly treatments with Certifect were effective in treating dogs with generalized demodicosis over a 3-month period. PMID:24256940

  19. Effect of ultraviolet irradiation on mast cell-deficient W/Wv mice

    SciTech Connect

    Ikai, K.; Danno, K.; Horio, T.; Narumiya, S.

    1985-07-01

    The effect of UV irradiation on the skin was investigated in (WB-W/+) X (C57BL/6J-Wv/+)F1-W/Wv mice, which are genetically deficient in tissue mast cells. Their congenic littermates (+/+) and normal albino mice (ICR or BALB/c) were used as controls. Mice were irradiated with 500 mJ/cm2 of UVB and the increment of ear thickness was measured before and 6, 12, and 24 h after irradiation. Ear swelling in W/Wv mice at 12 and 24 h after irradiation was significantly smaller than that in +/+ and ICR mice. In contrast, the number of sunburn cells formed 24 h after UVB irradiation (200 or 500 mJ/cm2) was similar in W/Wv, +/+ and ICR mice. On the other hand, when mice were treated with 8-methoxy-psoralen (0.5%) plus UVA irradiation (4 J/cm2) (topical PUVA), ears of W/Wv and BALB/c mice, which were both white in color, were thickened similarly 72 h after treatment, but less swelling was observed in +/+ mice, which were black in skin color. The amount of prostaglandin D2 (PGD2) in ears, determined by radioimmunoassay specific for PGD2, was elevated 3-fold in +/+ and ICR mice at 3 h after irradiation with 500 mJ/cm2 of UVB in comparison with basal level without irradiation. However, such elevation was not observed in W/Wv mice. These results suggest that mast cells play an important role in UVB-induced inflammation, and PGs from mast cells are responsible at least in part for the development of this reaction. However, neither mast cells nor PGs contribute to the sunburn cell formation and ear swelling response by PUVA treatment.

  20. 75 FR 65525 - TRG Insurance Solutions, Beckley, WV; Notice of Revised Determination on Reconsideration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-25

    ... Employment and Training Administration TRG Insurance Solutions, Beckley, WV; Notice of Revised Determination... of TRG Insurance Solutions, Beckley, West Virginia, to apply for Trade Adjustment Assistance. On... determine that workers of TRG Insurance Solutions, Beckley, West Virginia, who are engaged in...

  1. 16. Photocopy of published illustration, 1885; W.V. Herancourt, illustrator; J. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Photocopy of published illustration, 1885; W.V. Herancourt, illustrator; J. Monash, Minneapolis, publisher; SHOWS BIRDS-EYE VIEW OF DOWNTOWN MINNEAPOLIS WITH MILWAUKEE ROAD FREIGHT HOUSE LOCATED 2 INCHES FROM TOP AND LEFT MARGINS; LOOKING NORTHWEST - Chicago, Milwaukee, St. Paul & Pacific Railroad Freighthouse, 201-211 Third Avenue South, Minneapolis, Hennepin County, MN

  2. 75 FR 56469 - Safety Zone; Ohio River, Wheeling, WV, Wheeling Heritage Port Sternwheel Foundation Fireworks...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-16

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Ohio River, Wheeling, WV, Wheeling Heritage... Ohio River extending the full width of the river. The safety zone is needed to protect spectators and... Marker 90.5 on the Ohio River extending the full width of the river. The safety zone is needed to...

  3. Fabrication and characterization of MCC approved testing material: ATM-WV/205 glass

    SciTech Connect

    Maupin, G.D.; Bowen, W.M.; Daniel, J.L.

    1988-08-01

    The ATM-WV/205 glass was produced in accordance with PNL's QA Manual for License-Related Programs, MCC technical procedures, and MCC QA Plan that were in effect during the course of this work. The method and procedure to be used in the fabrication and characterization of the ATM-WV/205 glass were specified in two run plans for glass preparation and a characterization plan. The ATM-WV/205 glass meets all specifications. The elemental composition and oxidation state of the glass are within the sponsor's specifications. Visually, the ATM-WV/205 glass bars appear uniformly glassy and generally without exterior features. Microscopic examination and x-ray diffraction revealed low (about 0.5 wt %) concentrations of 3-..mu..m iron chrome spinel crystals and 1-..mu..m ruthenium inclusions scattered randomly throughout the glassy matrix. Closed porosity, with pores ranging in diameter from 20 to 135 ..mu..m, was observed in all samples. 3 refs., 10 figs., 21 tabs.

  4. Exploring Climate Science with WV Educators: A Regional Model for Teacher Professional Development

    NASA Astrophysics Data System (ADS)

    Ruberg, L. F.; Calinger, M.

    2014-12-01

    The National Research Council Framework for K-12 Science Literacy reports that children reared in rural agricultural communities, who experience regular interactions with plants and animals, develop more sophisticated understanding of ecology and biological systems than do urban and suburban children of the same age. West Virginia (WV) is a rural state. The majority of its residents live in communities of fewer than 2,500 people. Based on the features of the population being served and their unique strengths, this presentation focuses on a regional model for teacher professional development that addresses agricultural and energy vulnerabilities and adaptations to climate change in WV. The professional development model outlined shows how to guide teachers to use a problem-based learning approach to introduce climate data and analysis techniques within a scenario context that is locally meaningful. This strategy engages student interest by focusing on regional and community concerns. Climate science standards are emphasized in the Next Generation Science Standards, but WV has not provided its teachers with appropriate instructional resources to meet those standards. The authors addressed this need by offering a series of climate science education workshops followed by online webinars offered to WV science educators free of charge with funding by the West Virginia Space Grant Consortium. The authors report on findings from this series of professional development workshops conducted in partnership with the West Virginia Science Teachers Association. The goal was to enhance grades 5-12 teaching and learning about climate change through problem-based learning. Prior to offering the climate workshops, all WV science educators were asked to complete a short questionnaire. As Figure 1 shows, over 40% of the teacher respondents reported being confident in teaching climate science content. For comparison post workshops surveys measure teacher confidence in climate science

  5. 75 FR 1806 - Draft General Management Plan and Environmental Impact Statement, New River Gorge National River, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-13

    ... National Park Service Draft General Management Plan and Environmental Impact Statement, New River Gorge National River, WV AGENCY: National Park Service, Department of the Interior. ACTION: Notice of...), the National Park Service announces the availability of the ] Draft General Management Plan...

  6. 78 FR 72880 - Adequacy Status of the West Virginia Portion of the Steubenville-Weirton, WV-OH Nonattainment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-04

    ... findings through the transportation conformity adequacy process, under the Clean Air Act (CAA), for... of the Steubenville- Weirton, WV-OH 1997 PM 2.5 national ambient air quality standard (NAAQS... annual PM 2.5 air quality standard. DATES: Effective on December 19, 2013. FOR FURTHER...

  7. 78 FR 72881 - Adequacy Status of the West Virginia Portion of the Steubenville-Weirton, WV-OH Nonattainment...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-04

    ... findings through the transportation conformity adequacy process, under the Clean Air Act (CAA), for... of the Steubenville- Weirton, WV-OH 2006 PM 2.5 national ambient air quality standard (NAAQS... 2006 daily PM 2.5 air quality standard. DATES: Effective on December 19, 2013. FOR FURTHER...

  8. 7. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1880's. VOLCANO LITTLE THEATRE GUILD. SOME CAST. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  9. 19. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. THE OLD STILES HOUSE, LOOKING WEST. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  10. 9. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. VIEW LOOKING NORTH. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  11. 16. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. STILES RESIDENCE, THORNHILL FARM. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  12. 17. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. THE OLD STILES HOUSE LOOKING EAST. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  13. 5. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1880. VOLCANO TOWN HALL. BLACKLIN HOUSE AT LEFT. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  14. 1. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. VIEW LOOKING SOUTH. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  15. 14. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. MOUNT FARM OIL COMPANY. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  16. 12. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. THORNHILL STORE. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  17. Cross-language specialization in phonetic processing: English and Hindi perception of /w/-/v/ speech and nonspeech.

    PubMed

    Iverson, Paul; Wagner, Anita; Pinet, Melanie; Rosen, Stuart

    2011-11-01

    This study examined the perceptual specialization for native-language speech sounds, by comparing native Hindi and English speakers in their perception of a graded set of English /w/-/v/ stimuli that varied in similarity to natural speech. The results demonstrated that language experience does not affect general auditory processes for these types of sounds; there were strong cross-language differences for speech stimuli, and none for stimuli that were nonspeech. However, the cross-language differences extended into a gray area of speech-like stimuli that were difficult to classify, suggesting that the specialization occurred in phonetic processing prior to categorization.

  18. Interleukin 3 perfusion in W/Wv mice allows the development of macroscopic hematopoietic spleen colonies and restores cutaneous mast cell number

    SciTech Connect

    Ody, C.; Kindler, V.; Vassalli, P. )

    1990-07-01

    The genetically anemic W/Wv mice are characterized by the inability of their bone marrow cells to form macroscopic pluripotent hematopoietic colonies in the spleen of irradiated recipients upon transfer (colony-forming units). Furthermore, they almost totally lack mast cells, notably in the skin. In the present study, we have tested the effect of recombinant murine interleukin 3 (rmIL-3) on W/Wv mice hematopoiesis. Transfer of W/Wv bone marrow cells into lethally irradiated recipients perfused with rmIL-3 is followed by the appearance of macroscopic spleen colonies. Moreover, perfusion of rmIL-3 in W/Wv mice: (a) restores almost normal total numbers of hematopoietic precursors (colony-forming cells), but without modification of anemia; and (b) leads to the appearance of a normal number of mastocytes in the skin.

  19. U.S. DOE Industrial Technologies Program – Technology Delivery Plant-Wide Assessment at PPG Industries, Natrium, WV

    SciTech Connect

    Lester, Stephen R.; Wiethe, Jeff; Green, Russell; Guice, Christina; Gopalakrishnan, Bhaskaran; Turton, Richard

    2007-09-28

    PPG and West Virginia University performed a plantwide energy assessment at the PPG’s Natrium, WV chemical plant, an energy-intensive manufacturing facility producing chlor-alkali and related products. Implementation of all the assessment recommendations contained in this report could reduce plant energy consumption by 8.7%, saving an estimated 10,023,192 kWh/yr in electricity, 6,113 MM Btu/yr in Natural Gas, 401,156 M lb/yr in steam and 23,494 tons/yr in coal and reduce carbon dioxide emissions by 241 mm lb/yr. The total cost savings would amount to approximately $2.9 mm/yr. Projects being actively implemented will save $1.7 mm/yr; the remainder are undergoing more detailed engineering study.

  20. Region 5: Ohio Parkersburg Adequate Letter (4/18/2007)

    EPA Pesticide Factsheets

    This letter from EPA to the Ohio Environmental Protection Agency determined the 2009 and 2018 motor vehicle emission budgets for (MVEBs) for volatile organic compounds (VOCs) and oxides of nitrogen (NOx) for Washington County 8-hour ozone nonattainment

  1. 6. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. STAGE, VOLCANO TOWN HALL. OLD GLORY, GETTING THE WORST OF IT. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  2. 10. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    10. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV. EPISCOPAL CHURCH AND GATES RESIDENCE AT LEFT (n.d.). - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  3. 8. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. INTERIOR VOLCANO TOWN HALL FROM STAGE SHOWING RAISED SEATS. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  4. 3. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV. LOOKING DOWN THE CREEK, WEST BACK OF THE STORES, BEFORE THE FIRE (n.d.). - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  5. 2. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1898. LARGE TANK, VOLOCANO STATION AND BAND HOUSE, AFTER THE RR WAS DISMANTLED. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  6. 23. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1897. TAKEN AT WATER TANKS NEAR THE HIGH TRESTLE. BOB FLEMING-ENGINEER, WILSH (?) ROLLINS-FIREMAN, OTH (?) COLLINS-PASSENGER (WITH CANE). - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  7. 11. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. JOHN SHAFFER'S STORE AND JOHN WILSON'S BOWLING ALLEY AND SALOON IN FOREGROUND. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  8. 21. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV. 'JOHN NOON L(*(. SAYS HE DROVE THE FIRST SPIKE AND I SAW HIM PULL THE LAST ONE AT VOLCANO JUNCTION' SBS. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  9. 18. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1998. THIS ENGINE HAD CAB KNOCKED OFF AND TIRES USED ON OTHER ENGINE, SO JOHN NOON AND PAT O'BRIEN WERE SCRAPING IT. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  10. 13. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. VOLCANO SCHOOL HOUSE, ME. CHURCH IN WOODS AT LEFT. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  11. 22. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1898. TANK, VOLCANO PUMPING STATION, AND BARNE HOUSE STILL STANDING AND IN USE. THIS SHOWS THE OLD RR LINE. (1934 CAPTION). - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  12. 15. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, UP THE HILL, SOUTH OF THE TOWN (n.d.). - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  13. 24. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. JIM RIDGE'S CARPENTER SHOP, LOOKING UP THE CREEK, EAST. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  14. 4. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. CORNER OF THORNHILL STORE AND TOWN HALL. MRS. GATES AND JOHN SCHAFFER. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  15. 20. Photocopied 1973 from original owned by Albert M. Stiles, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. Photocopied 1973 from original owned by Albert M. Stiles, Jr., Parkersburg, WV, 1907. RESIDENCE OF E.W. STAPLES AND DR. W.H. SHARP. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  16. Category and perceptual interference in second-language phoneme learning: an examination of English /w/-/v/ learning by Sinhala, German, and Dutch speakers.

    PubMed

    Iverson, Paul; Ekanayake, Dulika; Hamann, Silke; Sennema, Anke; Evans, Bronwen G

    2008-10-01

    The present study investigated the perception and production of English /w/ and /v/ by native speakers of Sinhala, German, and Dutch, with the aim of examining how their native language phonetic processing affected the acquisition of these phonemes. Subjects performed a battery of tests that assessed their identification accuracy for natural recordings, their degree of spoken accent, their relative use of place and manner cues, the assimilation of these phonemes into native-language categories, and their perceptual maps (i.e., multidimensional scaling solutions) for these phonemes. Most Sinhala speakers had near-chance identification accuracy, Germans ranged from chance to 100% correct, and Dutch speakers had uniformly high accuracy. The results suggest that these learning differences were caused more by perceptual interference than by category assimilation; Sinhala and German speakers both have a single native-language phoneme that is similar to English /w/ and /v/, but the auditory sensitivities of Sinhala speakers make it harder for them to discern the acoustic cues that are critical to /w/-/v/ categorization.

  17. Two WV-MnIII bimetallic assemblies built by octacyanotungstate(v) and MnIII Schiff bases: molecular structures and a spin-flop transition.

    PubMed

    Ko, Hyun Hee; Lim, Jeong Hak; Yoo, Houng Sik; Kang, Jun Sung; Kim, Hyoung Chan; Koh, Eui Kwan; Hong, Chang Seop

    2007-05-28

    Two W(V)-Mn(III) bimetallic compounds, [Mn(Cl-salmen)(H(2)O)2]{[Mn(5-Clsalmen)(H(2)O)]2[W(CN)8].2H(2)O (1.2H(2)O) [5-Clsalmen = N,N'-(1-methylethylene)bis(5-chlorosalicylideneiminato) dianion], which contains trinuclear Mn(2)W and isolated Mn(III) moieties, and [Mn(3-MeOsalcy)(H(2)O)2]3[W(CN)(8)].2H(2)O (2.2H(2)O) [3-MeOsalcy = N,N'-(trans-1,2-cyclohexanediylethylene)bis(3-methoxysalicylideneiminato) dianion] molecules were prepared in redox processes and characterized using X-ray analysis and magnetic measurements. Compound 1 is composed of the {[Mn(5-Clsalmen)(H(2)O)]2[W(CN)8]}- trimer, in which two CN groups among eight in [W(CN)8](3-) bridge W(5+) and two Mn(3+) ions and the remaining CN ligands are hydrogen-bonded to water molecules or unbound, and the [Mn(Cl-salmen)(H(2)O)2]+ cation. Subsequently, two water molecules of the isolated cation are subject to hydrogen bonds. For 2, there are no covalent bonds among the subunits and six serial stacks of [Mn(3-MeOsalcy)(H(2)O)2]+ units are all hydrogen-bonded. The many hydrogen bonds found in both complexes eventually lead to three-dimensional networks. The magnetic studies for 1 reveal that antiferromagnetic interactions (J = -5.4 cm(-1)) between W(V) and Mn(III) centers within the trimer are transmitted via the bridging CN groups. Intermolecular antiferromagnetic couplings (zJ' = -0.2 cm(-1)) are also observed. The static and dynamic magnetic data of 1 demonstrate the existence of a field-induced spin-flop transition occurring among the clusters and monomeric molecules.

  18. Diluting ferric carboxymaltose in sodium chloride infusion solution (0.9% w/v) in polypropylene bottles and bags: effects on chemical stability

    PubMed Central

    Philipp, Erik; Braitsch, Michaela; Bichsel, Tobias; Mühlebach, Stefan

    2016-01-01

    Objectives This study was designed to assess the physicochemical stability of colloidal ferric carboxymaltose solution (Ferinject) when diluted and stored in polypropylene (PP) bottles and bags for infusion. Methods Two batches of ferric carboxymaltose solution (Ferinject) were diluted (500 mg, 200 mg and 100 mg iron in 100 mL saline) in PP bottles or bags under aseptic conditions. The diluted solutions were stored at 30°C and 75%±5% relative humidity (rH) for 72 h, and samples were withdrawn aseptically at preparation and after 24 h, 48 h and 72 h. Multiple parameters were used to test stability-related measures (pH, total iron and iron (II) content, molecular weight range determination, microbial contamination and particles count ≥10 μm). Results Overall, Ferinject diluted in 0.9% (w/v) NaCl solution and stored in PP bottles and bags was stable within the specifications for the complex and the acceptability limits set for all assays. In both containers, total iron content remained stable, within 10% of the theoretical iron content, and levels of iron (II) remained far below the threshold of acceptability. All preparations were free from sediments, particle numbers were acceptable and there was no microbial contamination. The molecular weight distribution and polydispersity index were also acceptable. Conclusions Under the tested experimental conditions, colloidal ferric carboxymaltose solution (Ferinject) diluted in saline in PP infusion bottles or bags demonstrated physical and chemical stability for up to 72 h at 30°C and 75% rH. Because of the lack of additional clinical data, when using ferric carboxymaltose, physicians/pharmacists should refer to the dilution and storing recommendations given in the product's summary of product characteristics. PMID:26835007

  19. Phenotypic changes of bone marrow-derived mast cells after intraperitoneal transfer into W/Wv mice that are genetically deficient in mast cells

    PubMed Central

    1987-01-01

    The ability of mouse IL-3-dependent, bone marrow culture-derived mast cells (BMMC) to generate serosal mast cells (SMC) in vivo after adoptive transfer to mast cell-deficient mice has been defined by chemical and immunochemical criteria. BMMC differentiated and grown from WBB6F1-+/+ mouse progenitor cells in medium containing PWM/splenocyte-conditioned medium synthesized a approximately 350,000 Mr protease-resistant proteoglycan bearing approximately 55,000 Mr glycosaminoglycans, as defined by gel filtration of each. Approximately 85% of the glycosaminoglycans bound to the cell-associated BMMC proteoglycans were chondroitin sulfates based upon their susceptibility to chondroitinase ABC digestion; HPLC of the chondroitinase ABC- generated unsaturated disaccharides revealed these glycosaminoglycans to be chondroitin sulfate E. As determined by heparinase and nitrous acid degradations, approximately 10% of the glycosaminoglycans bound to BMMC proteoglycans were heparin. In contrast, mast cells recovered from the peritoneal cavity of congenitally mast cell-deficient WBB6F1-W/Wv mice 15 wk after intraperitoneal injection of BMMC synthesized approximately 650,000 Mr protease-resistant proteoglycans that contained approximately 80% heparin glycosaminoglycans of approximately 105,000 Mr. Thus, after adoptive transfer, the SMC of the previously mast cell-deficient mice were like those recovered from the normal WBB6F1-+/+ mice that were shown to synthesize approximately 600,000 Mr proteoglycans that contained approximately 80% heparin glycosaminoglycans of approximately 115,000 Mr. As assessed by indirect immunofluorescence staining and flow cytometry using the B1.1 rat mAb (an antibody that recognizes an epitope located on the neutral glycosphingolipid globopentaosylceramide), approximately 5% of BMMC bound the antibody detectably, whereas approximately 72% of the SMC that were harvested from mast cell-deficient mice 15 wk after adoptive transfer of BMMC were B1.1-positive

  20. 12 CFR 615.5461 - Lost, stolen, destroyed, mutilated or defaced Farm Credit securities, including coupons.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... ADMINISTRATION FARM CREDIT SYSTEM FUNDING AND FISCAL AFFAIRS, LOAN POLICIES AND OPERATIONS, AND FUNDING OPERATIONS Book-Entry Procedures for Farm Credit Securities § 615.5461 Lost, stolen, destroyed, mutilated or... Public Debt, P.O. Box 396, Parkersburg, WV 26102-0396, in the case of consolidated or...

  1. 76 FR 26802 - Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-09

    .... Sharp, 200 Third Street, Avery 4-A, Parkersburg, WV 26106- 5312, or e-mail to Bruce.Sharp@bpd.treas.gov... instructions should be directed to Bruce A. Sharp, Bureau of the Public Debt, 200 Third Street, Avery 4-A... is requested to complete transaction involving the assignment of U.S. Registered and...

  2. 77 FR 32179 - Proposed Collection: Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-31

    ... Department of the Treasury is soliciting comments concerning the Certificate of Identity. DATES: Written..., Parkersburg, WV 26106-1328, (304) 480-8150. SUPPLEMENTARY INFORMATION: Title: Certificate of Identity. OMB... identity of the owner of the United States Savings Securities. Current Actions: None. Type of...

  3. ADAPTING EDUCATIONAL CHANGE TO MANPOWER NEEDS IN QUINCY, MASSACHUSETTS, AND WOOD COUNTY (PARKERSBURG), WEST VIRGINIA.

    ERIC Educational Resources Information Center

    ROGERS, VIRGIL M.

    PRESENT PROCEDURES AND INNOVATIONS RESULTING FROM TECHNOLOGICAL CHANGES WERE EXAMINED IN TWO REPRESENTATIVE SCHOOL SYSTEMS TO HELP OTHER SCHOOLS, BOARDS, AND FACULTIES FACING COMPARABLE PROBLEMS. THE STUDY FOCUSED ON MANPOWER TRAINING AND DEVELOPMENT, THE RESOURCES AVAILABLE IN THE COMMUNITY, THE PROFESSIONAL STAFF OF THE SCHOOLS, THE RECEPTIVITY…

  4. 49 CFR 372.207 - Charleston, WV.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... foreign commerce, not under common control, management, or arrangement for a continuous carriage or... this section, and (e) All of any municipality wholly surrounded, or so surrounded except for a...

  5. 49 CFR 372.215 - Ravenswood, WV.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... foreign commerce, not under common control, management, or arrangement for a continuous carriage or... the limits of the combined areas defined in paragraphs (b) and (c) of this section, and (e) All of...

  6. Studies on localization and function of annexin A4a within urinary bladder epithelium using a mouse knockout model.

    PubMed

    Hill, Warren G; Meyers, Susan; von Bodungen, Maximilian; Apodaca, Gerard; Dedman, John R; Kaetzel, Marcia A; Zeidel, Mark L

    2008-04-01

    Annexin A4 (anxA4) is a member of the Ca(2+)-dependent membrane-binding family of proteins implicated in the regulation of ion conductances, Ca(2+) homeostasis, and membrane trafficking. We demonstrate, in mice, that annexins 1-6 are present in whole bladder and exhibit differential expression in the urothelium. An anxA4a-knockout (anxA4a(-/-)) mouse model shows no protein in the urothelium by immunofluorescence and immunoblotting. In wild-type bladders, anxA4a in umbrella cells showed uniform cytoplasmic staining and some association with the nuclear membrane. Application of a hydrostatic pressure to bladders mounted in Ussing chambers resulted in redistribution of anxA4a from cytoplasm to cellular boundaries in the basal and intermediate cells but not in superficial umbrella cells. We hypothesized that anxA4a might be important for barrier function or for stretch-activated membrane trafficking. To test these hypotheses, we conducted a series of functional and morphological analyses on bladders from control and anxA4a(-/-) animals. The transepithelial resistances, water permeabilities, and urea permeabilities of anxA4a(-/-) bladders were not different from controls, indicating that barrier function was intact. Membrane trafficking in response to hydrostatic pressure as measured by capacitance increases was also normal for anxA4a(-/-) bladders. Cystometrograms performed on live animals showed that voiding frequency and intrabladder pressures were also not different. There were no differences in bladder surface morphology or cellular architecture examined by scanning and transmission electron microscopy, respectively. We conclude that loss of anxA4 from the urothelium does not affect barrier function, membrane trafficking, or normal bladder-voiding behavior.

  7. Evaluation of the Adulticidal Efficacy of Imidacloprid 10 %/Moxidectin 2.5 % (w/v) Spot-on (Advocate®, Advantage® Multi) against Dirofilaria repens in Experimentally Infected Dogs.

    PubMed

    Petry, Gabriele; Genchi, Marco; Schmidt, Holger; Schaper, Roland; Lawrenz, Bettina; Genchi, Claudio

    2015-08-01

    This study aimed to evaluate the efficacy of imidacloprid 10 %/moxidectin 2.5 % (w/v) spot-on (Advocate®/Advantage® Multi, Bayer) against adult Dirofilaria repens in a blinded, placebo-controlled randomised laboratory study. Twenty-four Beagle dogs were experimentally infected with approximately 75 infective D. repens larvae each on study day (SD) 0. Treatment was initiated on SD 228 after patency had been confirmed in 21 dogs, using a modified Knott Test. Eleven dogs received monthly treatments with imidacloprid/moxidectin at the minimum therapeutic dose (10 mg/kg imidacloprid and 2.5 mg/kg moxidectin) for six consecutive months and 12 control dogs were treated with a placebo formulation. Approximately one month after the last treatment, all dogs were euthanised and necropsied for the detection of D. repens worms. Eleven control dogs harboured live adult D. repens (range 2-11, geometric mean 5.44). Eight of 11 imidacloprid/moxidectin-treated dogs were free of live worms. The live worm count was reduced by 96.2 % (range 0-1, geometric mean 0.21). The majority of dead worms were encapsulated and degenerated. After the first treatment, Knott Tests were negative in all imidacloprid/moxidectin-treated dogs and this status was maintained in 10 dogs until study end. One dog showed a low microfilariae count (1 and 4/mL) on four occasions but was also negative before necropsy. The treatment was well tolerated by all study animals. It is concluded that six consecutive monthly treatments with imidacloprid/moxidectin spot-on are effective and safe against adult D. repens and provide an option for preventing the further spread of this zoonotic parasite.

  8. Inhibition of cytochrome P450 enzymes by saturated and unsaturated fatty acids in human liver microsomes, characterization of enzyme kinetics in the presence of bovine serum albumin (0.1 and 1.0% w/v) and in vitro - in vivo extrapolation of hepatic clearance.

    PubMed

    Palacharla, Raghava Choudary; Uthukam, Venkatesham; Manoharan, Arunkumar; Ponnamaneni, Ranjith Kumar; Padala, Nagasurya Prakash; Boggavarapu, Rajesh Kumar; Bhyrapuneni, Gopinadh; Ajjala, Devender Reddy; Nirogi, Ramakrishna

    2017-04-01

    The objective of the study was to determine the effect of fatty acids on CYP enzymes and the effect of BSA on intrinsic clearance of probe substrates. The inhibitory effect of thirteen fatty acids including saturated, mono-unsaturated and polyunsaturated fatty acids on CYP enzymes, kinetic parameters and intrinsic clearance values of nine CYP marker probe substrate reactions in the absence and presence of BSA (0.1 and 1.0% w/v) were characterized in human liver microsomes. The results demonstrate that most of the unsaturated fatty acids showed marked inhibition towards CYP2C8 mediated amodiaquine N-deethylation followed by inhibition of CYP2C9 and CYP2B6 mediated activities. The addition of 0.1% BSA in the incubation markedly improved the unbound intrinsic clearance values of probe substrates by reducing the Km values with little or no effect on maximal velocity. The addition of BSA (0.1 and 1.0% w/v) did not influence the unbound intrinsic clearance of marker reactions for CYP2A6, and CYP3A4 enzymes. The addition of 0.1% w/v BSA is sufficient to determine the intrinsic clearance of marker probe reactions by metabolite formation approach. The predicted hepatic clearance values for the substrates using the well-stirred model, in the presence of BSA (0.1% BSA), are comparable to the in vivo hepatic clearance values.

  9. Linkage and association of haplotypes at the APOA1/C3/A4/A5 genecluster to familial combined hyperlipidemia

    SciTech Connect

    Eichenbaum-Voline, Sophie; Olivier, Michael; Jones, Emma L.; Naoumova, Rossitza P.; Jones, Bethan; Gau, Brian; Seed, Mary; Betteridge,D. John; Galton, David J.; Rubin, Edward M.; Scott, James; Shoulders,Carol C.; Pennacchio, Len A.

    2002-09-15

    Combined hyperlipidemia (CHL) is a common disorder of lipidmetabolism that leads to an increased risk of cardiovascular disease. Thelipid profile of CHL is characterised by high levels of atherogeniclipoproteins and low levels of high-density-lipoprotein-cholesterol.Apolipoprotein (APO) A5 is a newly discovered gene involved in lipidmetabolism located within 30kbp of the APOA1/C3/A4 gene cluster. Previousstudies have indicated that sequence variants in this cluster areassociated with increased plasma lipid levels. To establish whethervariation at the APOA5 gene contributes to the transmission of CHL, weperformed linkage and linkage disequilibrium (LD) tests on a large cohortof families (n=128) with familial CHL (FCHL). The linkage data producedevidence for linkage of the APOA1/C3/A4/A5 genomic interval to FCHL (NPL= 1.7, P = 0.042). The LD studies substantiated these data. Twoindependent rare alleles, APOA5c.56G and APOC3c.386G of this gene clusterwere over-transmitted in FCHL (P = 0.004 and 0.007, respectively), andthis was associated with a reduced transmission of the most commonAPOA1/C3/A4/A5 haplotype (frequency 0.4425) to affected subjects (P =0.013). The APOA5c.56G allele was associated with increased plasmatriglyceride levels in FCHL probands, whereas the second, andindependent, APOC3c.386G allele was associated with increased plasmatriglyceride levels in FCHL pedigree founders. Thus, this allele (or anallele in LD) may mark a quantitative trait associated with FCHL, as wellas representing a disease susceptibility locus for the condition. Thisstudy establishes that sequence variation in the APOA1/C3/A4/A5 genecluster contributes to the transmission of FCHL in a substantialproportion of affected families, and that these sequence variants mayalso contribute to the lipid abnormalities of the metabolic syndrome,which is present in up to 40 percent of persons with cardiovasculardisease.

  10. Orthologs of the class A4 heat shock transcription factor HsfA4a confer cadmium tolerance in wheat and rice.

    PubMed

    Shim, Donghwan; Hwang, Jae-Ung; Lee, Joohyun; Lee, Sichul; Choi, Yunjung; An, Gynheung; Martinoia, Enrico; Lee, Youngsook

    2009-12-01

    Cadmium (Cd) is a widespread soil pollutant; thus, the underlying molecular controls of plant Cd tolerance are of substantial interest. A screen for wheat (Triticum aestivum) genes that confer Cd tolerance to a Cd hypersensitive yeast strain identified Heat shock transcription factor A4a (HsfA4a). Ta HsfA4a is most similar to the class A4 Hsfs from monocots. The most closely related rice (Oryza sativa) homolog, Os HsfA4a, conferred Cd tolerance in yeast, as did Ta HsfA4a, but the second most closely related rice homolog, Os HsfA4d, did not. Cd tolerance was enhanced in rice plants expressing Ta HsfA4a and decreased in rice plants with knocked-down expression of Os HsfA4a. An analysis of the functional domain using chimeric proteins constructed from Ta HsfA4a and Os HsfA4d revealed that the DNA binding domain (DBD) of HsfA4a is critical for Cd tolerance, and within the DBD, Ala-31 and Leu-42 are important for Cd tolerance. Moreover, Ta HsfA4a-mediated Cd resistance in yeast requires metallothionein (MT). In the roots of wheat and rice, Cd stress caused increases in HsfA4a expression, together the MT genes. Our findings thus suggest that HsfA4a of wheat and rice confers Cd tolerance by upregulating MT gene expression in planta.

  11. Effects of variations in the APOA1/C3/A4/A5 gene cluster on different parameters of postprandial lipid metabolism in healthy young men

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Background: The APOA1/C3/A4/A5 gene cluster encodes important regulators of fasting lipids, but the majority of lipid metabolism takes place in the postprandial state, and knowledge about gene regulation in this state is scarce. With the aim of characterizing possible regulators of lipid metabolism...

  12. The APOA1/C3/A4/A5 cluster and markers of allostatic load in the Boston Puerto Rican Health Study

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The APOA1/C3/A4/A5 cluster encodes key regulators of plasma lipids. Interactions between dietary factors and single nucleotide polymorphisms (SNPs) in the cluster have been reported. Allostatic load, or physiological dysregulation in response to stress, has been implicated in shaping health disparit...

  13. 76 FR 34606 - Special Local Regulation; Monongahela River, Morgantown, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-14

    ... safeguard participants during the Mountaineer Triathlon marine event from the hazards imposed by marine... regulation is being established to safeguard participants of the Mountaineer Triathlon from the hazards of marine traffic. Entry into, movement within, and departure from this Coast Guard regulated area...

  14. Argus Energy WV, LLC wins 2007 Wetlands West Virginia Award

    SciTech Connect

    2007-07-15

    Argus Energy's Kiah Creek Operation has received the 2007 Wetlands West Virginia Award presented by the West Virginian Coal Association. The operation was originally a 1267 acre underground mine in the Coalburg seam. Underground mining commenced in 2000 until the end of 2003 with more than two million tons of coal being produced. The creation of the wetlands was achieved during the operations. 8 photos.

  15. 77 FR 3836 - Supplemental Environmental Impact Statement, Mingo County, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-25

    ... portion of the King Coal Highway 2000 FEIS and the Buffalo Mountain Surface Mine Clean Water Act Section... USACE is evaluating a Clean Water Act (CWA) Section 404 Individual Permit (IP) application submitted by....S.) in conjunction with the construction and operation of the Buffalo Mountain Surface Mine....

  16. 75 FR 22871 - West Virginia Disaster # WV-00018

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-30

    ... snowstorms. Incident Period: 02/05/2010 through 02/11/2010. Effective Date: 04/23/2010. Physical Loan Application Deadline Date: 06/22/2010. Economic Injury (EIDL) Loan Application Deadline Date: 01/24/2011... P. Loddo, Acting Associate Administrator for Disaster Assistance. BILLING CODE 8025-01-P...

  17. Nobody Asked Us: The WV Welfare Reform Listening Project.

    ERIC Educational Resources Information Center

    Wilson, Rick

    West Virginia's state welfare reform plan was in effect by the end of 1997. In 1999, over 175 interviews were conducted with past and present welfare recipients to bring their experiences and concerns to the attention of the public and policymakers. Nearly all interviewees were women and were taking part in work activities. In general, welfare…

  18. Nigellamines A3, A4, A5, and C, new dolabellane-type diterpene alkaloids, with lipid metabolism-promoting activities from the Egyptian medicinal food black cumin.

    PubMed

    Morikawa, Toshio; Xu, Fengming; Ninomiya, Kiyofumi; Matsuda, Hisashi; Yoshikawa, Masayuki

    2004-04-01

    New dolabellane-type diterpene alkaloids, nigellamines A(3), A(4), A(5), and C, were isolated from the methanolic extract of an Egyptian medicinal food, black cumin (the seeds of Nigella sativa). Their absolute configurations were determined on the basis of chemical and physicochemical evidence. Nigellamines were found to lower triglyceride levels in primary cultured mouse hepatocytes, and in particular, the activity of nigellamine A(5) was equivalent to that of the hypolipidemic agent, clofibrate.

  19. Sputtering Deposition of Sandwich-Structured V2O5/Metal (V, W)/V2O5 Multilayers for the Preparation of High-Performance Thermally Sensitive VO2 Thin Films with Selectivity of VO2 (B) and VO2 (M) Polymorph.

    PubMed

    Liu, Hengwu; Wan, Dongyun; Ishaq, Ahmad; Chen, Lanli; Guo, Beibei; Shi, Siqi; Luo, Hongjie; Gao, Yanfeng

    2016-03-01

    For specific application to an uncooled infrared detector, VO2 thin films should have a series of characteristics including purposefully chosen polymorphs, accurate stoichiometry, phase stabilization, a high temperature-coefficient of resistance (TCR), and suitable square-resistance. This work reports controllable preparation of high-performance VO2 films via post annealing of a sandwich-structured V2O5/metal (V, W)/V2O5 multilayer precursor, which was deposited by RF magnetron sputtering. This sandwich structure can dynamically regulate oxygen contents and doping element levels in the films, enabling us to achieve accurate regulation of stoichiometry and polymorphs. The precursor films undergo a B to M phase transition depending on the quantity of the metal layers. At the thickness of the metal layer below a limitation, the resulting film after heat treatment was VO2 (B), and above the limitation, the product was VO2 (M). The optical modulation of the VO2 (M) in the near-infrared region can be tuned from 1.2 to 39.8% (ΔT2000 nm). TCR values can range from -1.89 to -4.29%/K and the square-resistances at room temperature (R0) from 69.68 to 12.63 kΩ. The simplicity in phase regulation of the present method and the superior optical and electrical properties of the films may allow its wide applications in thermo-opto-electro sensing devices.

  20. Molecules based on M(v) (M=Mo, W) and Ni(II) ions: a new class of trigonal bipyramidal cluster and confirmation of SMM behavior for the pentadecanuclear molecule {NiII[NiII(tmphen)(MeOH)]6[Ni(H2O)3]2[micro-CN]30[WV(CN)3]6}.

    PubMed

    Hilfiger, Matthew G; Zhao, Hanhua; Prosvirin, Andrey; Wernsdorfer, Wolfgang; Dunbar, Kim R

    2009-07-14

    The preparation, single crystal X-ray crystallography, and magnetic properties are reported for four new clusters based on [M'V(CN)8]3- octacyanometallates (M'=Mo, W). Reactions of [M'V(CN)8]3- with mononuclear NiII ions in the presence of the tmphen blocking ligand (tmphen=3,4,7,8-tetramethyl-1,10-phenanthroline) in a 2:3:6 ratio, respectively, lead to the formation of the trigonal bipyramidal clusters [NiII(tmphen)2]3[M'V(CN)8]2. Analogous reactions with the same starting materials performed in a 2:3:2 ratio, respectively, produce pentadecanuclear clusters of the type {NiII[NiII(tmphen)(MeOH)]6[Ni(H2O)3]2[micro-CN]30[WV(CN)3]6}. The W2Ni3 (1) and Mo2Ni3(2) pentanuclear clusters and the W6Ni9 (3) and Mo6Ni9 (4) pentadecanuclear molecules are isostructural to each other and crystallize in the space groups P2(1)/c and R3 respectively. Magnetic measurements indicate that the ground states for the trigonal bipyamidal clusters are S=4 as a consequence of ferromagnetic coupling with JW-Ni=9.5 cm(-1), JMo-Ni=10 cm(-1). The pentadecanuclear clusters exhibit ferromagnetic coupling as well, which leads to S=12 ground states (JW-Ni=12 cm(-1), JMo-Ni=12.2 cm(-1)). Reduced magnetization studies on the W-Ni analogues support the conclusion that they exhibit a negative axial anisotropy term; the fits give D values of -0.24 cm(-1) for the W2Ni3 cluster and D=-0.04 cm(-1)for the W6Ni9 cluster. AC susceptibility measurements indicate the beginning of an out-of-phase signal for the W2Ni3 and the W6Ni9 compounds, but detailed low temperature studies on small crystals by the microSQUID technique indicate that only the pentadecanuclear cluster exhibits hysteresis in accord with SMM behavior. Neither Mo cluster reveals any evidence for slow paramagnetic relaxation at low temperatures.

  1. Haplotypes in the APOA1-C3-A4-A5 gene cluster affect plasma lipids in both humans and baboons

    SciTech Connect

    Wang, Qian-fei; Liu, Xin; O'Connell, Jeff; Peng, Ze; Krauss, Ronald M.; Rainwater, David L.; VandeBerg, John L.; Rubin, Edward M.; Cheng, Jan-Fang; Pennacchio, Len A.

    2003-09-15

    Genetic studies in non-human primates serve as a potential strategy for identifying genomic intervals where polymorphisms impact upon human disease-related phenotypes. It remains unclear, however, whether independently arising polymorphisms in orthologous regions of non-human primates leads to similar variation in a quantitative trait found in both species. To explore this paradigm, we studied a baboon apolipoprotein gene cluster (APOA1/C3/A4/A5) for which the human gene orthologs have well established roles in influencing plasma HDL-cholesterol and triglyceride concentrations. Our extensive polymorphism analysis of this 68 kb gene cluster in 96 pedigreed baboons identified several haplotype blocks each with limited diversity, consistent with haplotype findings in humans. To determine whether baboons, like humans, also have particular haplotypes associated with lipid phenotypes, we genotyped 634 well characterized baboons using 16 haplotype tagging SNPs. Genetic analysis of single SNPs, as well as haplotypes, revealed an association of APOA5 and APOC3 variants with HDL cholesterol and triglyceride concentrations, respectively. Thus, independent variation in orthologous genomic intervals does associate with similar quantitative lipid traits in both species, supporting the possibility of uncovering human QTL genes in a highly controlled non-human primate model.

  2. 76 FR 64295 - Proposed Amendment of Class E Airspace; Huntington, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-18

    ...-800-647-5527; Fax: 202-493-2251. You must identify the Docket Number FAA-2011-1057; Airspace Docket No... the proposal. Communications should identify both docket numbers (FAA Docket No. FAA-2011-1057... made: ``Comments to Docket No. FAA-2011-1057; Airspace Docket No. 11-AEA-21.'' The postcard will...

  3. Characterization of Rain Attenuation and Depolarization at W/V Bands

    DTIC Science & Technology

    2015-07-30

    5. M. Islam, Z. Elabdin, O. Elshaikh, O. Khalifa, A. Zahirul, S. Khan, and A. Naji. “Prediction of Signal Attenuation Due to Dust Storms Using Mie...from a Derived Two-Dimensional Dust Storm Model,” IEEE Trans. Antennas Propagation, Vol. 49, No. 12, pp. 1703-1711, 2001. 7. Study Group 3 – Radiowave...8-12], which causes uncertainty in the correlation of attenuation and rain-rate measurements. Furthermore, the overall dielectric constant of

  4. WV R-EMAP STUDY: MULTIPLE-OBJECTIVE SAMPLING DESIGN FRAMEWORK

    EPA Science Inventory

    A multi-objective sampling design has been implemented through Regional Monitoring and Assessment Program (R-EMAP) support of a cooperative agreement with the state of West Virginia. Goals of the project include: 1) development and testing of a temperature-adjusted fish IBI for t...

  5. 78 FR 8589 - Verizon Services Corporation, Customer Services Clerk, General Clerk, Clarksburg, WV; Notice of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ... Employment and Training Administration Verizon Services Corporation, Customer Services Clerk, General Clerk... of Verizon Services Corporation, Customer Services Clerk, General Clerk, Clarksburg, West Virginia... country the supply of services like or directly competitive with those supplied by the workers and did...

  6. 77 FR 44684 - American Woodmark Corporation, Moorefield, WV; Notice of Negative Determination on Reconsideration

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-30

    ... kitchen and bath cabinetry products. Pursuant to 29 CFR 90.18(c), reconsideration may be granted under the... were not attributable to increased imports of kitchen and bath cabinetry, or articles like or directly... to a shift of production of kitchen and bath cabinetry, or articles like or directly competitive,...

  7. 75 FR 63540 - Request for Public Comment, Morgantown Municipal Airport, Morgantown, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-15

    ... Sponsor's operation and development of the Morgantown Airport. Once released, the land will change to a non-aeronautical use and will be transferred to the West Virginia Army National Guard (``AR- WVARNG... Guard Readiness Center on the property. The development of this property will also bring...

  8. 78 FR 3964 - Request for Public Comment, Raleigh County Memorial Airport, Beckley, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-17

    ... Creek Gorge'' to a depth in excess of 600ft below the airport elevation and has no aeronautical benefit... development opportunities exist for the airport. Once released, the land will be sold and placed in a Conservation Easement, with restriction of no future development. Proposed buyer would be placing the area...

  9. 76 FR 12365 - Canaan Valley National Wildlife Refuge, Tucker and Grant Counties, WV; Final Comprehensive...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-07

    ...-forest matrix landscape with the management of early successional habitats and the protection of wetlands... refuge. Management would range from passive, or ``letting nature take its course,'' to actively... natural disturbances such as infrequent fires, ice storms, and small patch blow-downs. Under...

  10. 77 FR 38376 - Request for Public Comment, Raleigh County Memorial Airport, Beckley, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-27

    ... Airport Circle, Room 101, Beaver, West Virginia 25813. In addition, one copy of any comments submitted to... Airport Circle, Room 105, Beaver, West Virginia 25813. FOR FURTHER INFORMATION CONTACT: Connie...

  11. 76 FR 40402 - Matthews International Corporation, Bronze Division, Kingwood, WV; Notice of Negative...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-08

    ... Employment and Training Administration TA-W-72,953 Matthews International Corporation, Bronze Division... former workers of Matthews International Corporation, Bronze ] Division, Kingwood, West Virginia (subject..., 2011 (76 FR 7584). Workers were engaged in the production of cast bronze memorial products. The...

  12. Proceedings of the Third International Workshop on Multistrategy Learning, May 23-25 Harpers Ferry, WV.

    DTIC Science & Technology

    1996-09-16

    strategies and paradigms, cognitive models of learning processes and their relationships to methods and-paradigms of machine learning, the development of...learning used. methods according to a set of "knowledge transmutation" processes . A toolbox with selection approach is described in Independently of a...taking subformulas in the specified hypothesis language. During into consideration global information. this process , the domain theory may also be

  13. First highly efficient and photostable E and C derivatives of 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) as dye lasers in the liquid phase, thin films, and solid-state rods.

    PubMed

    Duran-Sampedro, Gonzalo; Esnal, Ixone; Agarrabeitia, Antonia R; Bañuelos Prieto, Jorge; Cerdán, Luis; García-Moreno, Inmaculada; Costela, Angel; Lopez-Arbeloa, Iñigo; Ortiz, María J

    2014-02-24

    A new library of E- and C-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) derivatives has been synthesized through a straightforward protocol from commercially available BODIPY complexes, and a systematic study of the photophysical properties and laser behavior related to the electronic properties of the B-substituent group (alkynyl, cyano, vinyl, aryl, and alkyl) has been carried out. The replacement of fluorine atoms by electron-withdrawing groups enhances the fluorescence response of the dye, whereas electron-donor groups diminish the fluorescence efficiency. As a consequence, these compounds exhibit enhanced laser action with respect to their parent dyes, both in liquid solution and in the solid phase, with lasing efficiencies under transversal pumping up to 73 % in liquid solution and 53 % in a solid matrix. The new dyes also showed enhanced photostability. In a solid matrix, the derivative of commercial dye PM597 that incorporated cyano groups at the boron center exhibited a very high lasing stability, with the laser emission remaining at the initial level after 100 000 pump pulses in the same position of the sample at a 10 Hz repetition rate. Distributed feedback laser emission was demonstrated with organic films that incorporated parent dye PM597 and its cyano derivative. The films were deposited onto quartz substrates engraved with appropriate periodical structures. The C derivative exhibited a laser threshold lower than that of the parent dye as well as lasing intensities up to three orders of magnitude higher.

  14. 47 CFR 22.627 - Effective radiated power limits.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    .... Pleasant, MI, 43°34′24.1″ N. Lat. 84°46′21.1″ W. Long. 476-482 MHz482-488 MHz 488-494 MHz 494-500 MHz 500.... Lat. 77°00′15.9″ W. Long. Champaign, IL, 40°04′11.1″ N. Lat. 87°54′45.1″ W. Long. Madison, WI, 43°03′01.0″ N. Lat. 89°29′15.4″ W. Long. Parkersburg, WV, 39°20′50.3″ N. Lat. 81°33′55.5″ W. Long....

  15. 47 CFR 22.627 - Effective radiated power limits.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    .... Pleasant, MI, 43°34′24.1″ N. Lat. 84°46′21.1″ W. Long. 476-482 MHz482-488 MHz 488-494 MHz 494-500 MHz 500.... Lat. 77°00′15.9″ W. Long. Champaign, IL, 40°04′11.1″ N. Lat. 87°54′45.1″ W. Long. Madison, WI, 43°03′01.0″ N. Lat. 89°29′15.4″ W. Long. Parkersburg, WV, 39°20′50.3″ N. Lat. 81°33′55.5″ W. Long....

  16. 47 CFR 22.627 - Effective radiated power limits.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    .... Pleasant, MI, 43°34′24.1″ N. Lat. 84°46′21.1″ W. Long. 476-482 MHz482-488 MHz 488-494 MHz 494-500 MHz 500.... Lat. 77°00′15.9″ W. Long. Champaign, IL, 40°04′11.1″ N. Lat. 87°54′45.1″ W. Long. Madison, WI, 43°03′01.0″ N. Lat. 89°29′15.4″ W. Long. Parkersburg, WV, 39°20′50.3″ N. Lat. 81°33′55.5″ W. Long....

  17. 47 CFR 22.627 - Effective radiated power limits.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    .... Pleasant, MI, 43°34′24.1″ N. Lat. 84°46′21.1″ W. Long. 476-482 MHz482-488 MHz 488-494 MHz 494-500 MHz 500.... Lat. 77°00′15.9″ W. Long. Champaign, IL, 40°04′11.1″ N. Lat. 87°54′45.1″ W. Long. Madison, WI, 43°03′01.0″ N. Lat. 89°29′15.4″ W. Long. Parkersburg, WV, 39°20′50.3″ N. Lat. 81°33′55.5″ W. Long....

  18. Specificity of the hepatitis C virus NS3 serine protease: effects of substitutions at the 3/4A, 4A/4B, 4B/5A, and 5A/5B cleavage sites on polyprotein processing.

    PubMed Central

    Kolykhalov, A A; Agapov, E V; Rice, C M

    1994-01-01

    Cleavage at four sites (3/4A, 4A/4B, 4B/5A, and 5A/5B) in the hepatitis C virus polyprotein requires a viral serine protease activity residing in the N-terminal one-third of the NS3 protein. Sequence comparison of the residues flanking these cleavage sites reveals conserved features including an acidic residue (Asp or Glu) at the P6 position, a Cys or Thr residue at the P1 position, and a Ser or Ala residue at the P1' position. In this study, we used site-directed mutagenesis to assess the importance of these and other residues for NS3 protease-dependent cleavages. Substitutions at the P7 to P2' positions of the 4A/4B site had varied effects on cleavage efficiency. Only Arg at the P1 position or Pro at P1' substantially blocked processing at this site. Leu was tolerated at the P1 position, whereas five other substitutions allowed various degrees of cleavage. Substitutions with positively charged or other hydrophilic residues at the P7, P3, P2, and P2' positions did not reduce cleavage efficiency. Five substitutions examined at the P6 position allowed complete cleavage, demonstrating that an acidic residue at this position is not essential. Parallel results were obtained with substrates containing an active NS3 protease domain in cis or when the protease domain was supplied in trans. Selected substitutions blocking or inhibiting cleavage at the 4A/4B site were also examined at the 3/4A, 4B/5A, and 5A/5B sites. For a given substitution, a site-dependent gradient in the degree of inhibition was observed, with a 3/4A site being least sensitive to mutagenesis, followed by the 4A/4B, 4B/5A, and 5A/5B sites. In most cases, mutations abolishing cleavage at one site did not affect processing at the other serine protease-dependent sites. However, mutations at the 3/4A site which inhibited cleavage also interfered with processing at the 4B/5A site. Finally, during the course of these studies an additional NS3 protease-dependent cleavage site has been identified in the NS4B

  19. The search for extraterrestrial intelligence; Proceedings of the Workshop, Green Bank, WV, May 20-22, 1985

    NASA Astrophysics Data System (ADS)

    Kellermann, Kenneth I.; Seielstad, George A.

    Papers are presented on life in space and humanity on earth, the Ozma project, the evolution of strategy for SETI, the search for biomolecules in space, a symbiotic SETI search, searches in the optical portion of the spectrum, a search for SETI targets, and a Milky Way search strategy for extraterrestrial intelligence. Topics discussed include SETI observations worldwide, the ultranarrow-band SETI at Harvard/Smithsonian, the NASA SETI program, narrow-band signal detection in the SETI field test, radio astronomy aspects of the NASA SETI sky survey, and small SETI systems. Consideration is given to the nature of technological civilizations and the manifestation of intelligence implications for SETI.

  20. Superfund Record of Decision (EPA Region 3): Allegany Ballistics Laboratory (USNavy), Operable Unit 1, Mineral, WV, February 12, 1997

    SciTech Connect

    1998-01-01

    This decision document presents the selected remedial action for Site 5 Landfill Contents and Surface Soil at the Allegany Ballistics Laboratory (ABL), Rocket Center, West Virginia. The major components of the selected remedy are: Deed notation along with property use and limited access restrictions; Installation of a composite CAP-GCL and FMC; Installation of a drainage layer utilizing a geonet; Installation of a passive landfill gas (LFG) venting system; Revegetation of the capped area; Installation of perimeter drainage system; and Post-closure requirements.

  1. Superfund record of decision (EPA Region 3): Allegany Ballistics Laboratory (USNavy) Site 10, Mineral, WV, June 30, 1998

    SciTech Connect

    1998-12-01

    This decision document presents the selected remedial action for Site 10 (the Site) Groundwater at the Allegany Ballistics Laboratory (ABL), Rocket Center, West Virginia. The major components of the selected remedy are: Institutional controls, including land use restrictions imposed through appropriate administrative mechanisms to prevent groundwater use; Groundwater pumping from a minimum of three extraction wells to capture the hot spot of the VOC contaminant plume; Installation of a pipeline to transport groundwater from Site 10 to the Site 1 treatment plant; Discharge to the North Branch Potomac River; and Groundwater monitoring on a timely basis, quarterly to semi-annually, will evaluate groundwater quality, contaminant migration, and degradation for inclusion in the 5-year site reviews.

  2. Feasibility study of wood-fired cogeneration at a Wood Products Industrial Park, Belington, WV. Phase II

    SciTech Connect

    Vasenda, S.K.; Hassler, C.C.

    1992-06-01

    Customarily, electricity is generated in a utility power plant while thermal energy is generated in a heating/cooling plant; the electricity produced at the power plant is transmitted to the heating/cooling plant to power equipments. These two separate systems waste vast amounts of heat and result in individual efficiencies of about 35%. Cogeneration is the sequential production of power (electrical or mechanical) and thermal energy (process steam, hot/chilled water) from a single power source; the reject heat of one process issued as input into the subsequent process. Cogeneration increases the efficiency of these stand-alone systems by producing these two products sequentially at one location using a small additional amount of fuel, rendering the system efficiency greater than 70%. This report discusses cogeneration technologies as applied to wood fuel fired system.

  3. Bayer Cropscience to Enhance Safeguards at Chemical Facilities in Four States to Settle Violations at W.V. Plant

    EPA Pesticide Factsheets

    WASHINGTON - The U.S. Environmental Protection Agency (EPA) and U.S. Department of Justice (DOJ) today announced a $5.6 million settlement with Bayer CropScience LP to resolve violations of federal chemical accident prevention laws at its facility i

  4. Rock Physics Modeling to Constrain Petrophysical Properties in the Productive Zone of the Marcellus Shale, WV from Wireline Log Data

    NASA Astrophysics Data System (ADS)

    Morshed, S. M.; Tatham, R. H.

    2013-12-01

    A rock physics characterization based on wireline log data is proposed for constraining the petrophysical properties of the productive interval in the Marcellus Shale. The method involves two parts, 1) petrophysical interpretation of organic shale from wireline log data, and 2) rock physics modeling utilizing the interpreted log data. A petrophysical interpretation of the more radioactive interval of log data suggests that higher TOC is associated with lower clay content. This interpretation also showed that upper the part of the Marcellus Shale is clay dominated whereas the lower part is quartz dominated. Following the petrophysically interpreted data, the rock physics modeling was performed using differential effective medium (DEM) scheme in an inclusion based model to estimate the effective elastic moduli of the composites. The elastic moduli of the matrix phase in the DEM were provided with the Voigt-Reuss-Hill average for a composition of quartz and clay. Imbedded inclusions were assumed. Three types of inclusion phases were considered; a dry pore (i.e. equant pores or ellipsoidal pores), a water-wet clay pore and kerogen. Dry pores were saturated with pore fluids simulating reservoir situations with the low frequency Gassmann equations. Rock physics modeling suggests that the elastic properties of the Marcellus Shale were controlled by the interplay of clay content, kerogen content and low aspect ratio pores. Low aspect ratio pores (~1/40) also comprise the dominant pore types in the Marcellus Shale and these pores are more common in the lower part of the formation. An illustration of the DEM scheme for understanding the effect of inclusion moduli and inclusion shape to host material. Here, the host is a solid rock of 40% Quartz with clay mixture, and kerogen is the inclusion. It shows that aspect ratio of kerogen inclusion plays a significant role in this scheme; lower aspect ratio produces much lower elastic moduli than higher aspect ratios.

  5. Effects of a Culturally Responsive Teaching Project on Teachers and Students in Selected Kanawha County, WV, Schools

    ERIC Educational Resources Information Center

    Hughes, Georgia K.; Cowley, Kimberly S.; Copley, Lisa D.; Finch, Nicole L.; Meehan, Merrill L.; Burns, Rebecca C.; Kusimo, Patricia S.; Keyes, Marian C.; Orletsky, Sandra R.; Holdzkom, David

    2004-01-01

    Differences in academic achievement among ethnic and socioeconomic groups, called achievement gaps, have been an issue in education for many years. Achievement gaps exist between upper- and lower-class students and between students of differing races and ethnic backgrounds. As a group, Black and Hispanic students perform less well on many…

  6. 75 FR 47715 - Safety Zone; Kanawha River Mile 56.7 to 57.6, Charleston, WV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-09

    ... Governor's Cup Jet Ski Race. Entry into this zone is prohibited unless specifically authorized by the... ``Search.'' They are also available for inspection or copying at the Docket Management Facility (M-30), U.S... under section 4(a) of the Administrative Procedure Act (APA) (5 U.S.C. 553(b)). This...

  7. WV R-EMAP SMALL WATERSHED CHARACTERIZATION, CLASSIFICATION, AND ASSESSMENT FOR WEST VIRGINIA UTILIZING EMAP DESIGN AND TOOLS

    EPA Science Inventory

    A probabilistic watershed-based framework was developed to encompass wadeable streams within all three ecoregions of West Virginia, with the exclusion noted below. In Phase I of the project (year 2001), we developed and applied a probabilistic watershed-based sampling framework ...

  8. 76 FR 57013 - Approval and Promulgation of Air Quality Implementation Plans; West Virginia; Revised Motor...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-15

    ...; Revised Motor Vehicle Emission Budgets for the Charleston, Huntington, Parkersburg, Weirton, and Wheeling..., Huntington, Parkersburg, Weirton, and Wheeling 8-hour ozone maintenance areas. This revision amends the... Federal Register, EPA is approving the State's SIP submittal as a direct final rule without prior...

  9. 76 FR 79539 - Approval and Promulgation of Air Quality Implementation Plans; West Virginia; Revised Motor...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-22

    ...; Revised Motor Vehicle Emission Budgets for the Charleston, Huntington, Parkersburg, Weirton, and Wheeling... a direct final rule pertaining to EPA's approval of the revised motor vehicle emissions budgets for the Charleston, Huntington, Parkersburg, Weirton, and Wheeling 8-hour ozone maintenance areas....

  10. Arthurdale (WV), Its Community School, and Director Elsie Ripley Clapp (1879-1965): First New Deal Subsistence Homestead Program (1933-48).

    ERIC Educational Resources Information Center

    Parker, Franklin

    Arthurdale, in northern West Virginia, was the first New Deal subsistence homestead project. Begun in 1933, the Arthurdale settlement was created to house displaced and jobless coal mining families. Eleanor Roosevelt was very active in the Arthurdale project. At the time of the project's founding, it was attacked by some for its costliness and the…

  11. RETROFIT COSTS FOR SO2 AND NOX CONTROL OPTIONS AT 200 COAL-FIRED PLANTS, VOLUME V - SITE SPECIFIC STUDIES FOR PA, SC, TN, VA, WI, WV

    EPA Science Inventory

    The report gives results of a study, the objective of which was to significantly improve engineering cost estimates currently being used to evaluate the economic effects of applying SO2 and NOx controls at 200 large SO2-emitting coal-fired utility plants. To accomplish the object...

  12. A Watershed-based spatially-explicit demonstration of an Integrated Environmental Modeling Framework for Ecosystem Services in the Coal River Basin (WV, USA)

    EPA Science Inventory

    We demonstrate a spatially-explicit regional assessment of current condition of aquatic ecoservices in the Coal River Basin (CRB), with limited sensitivity analysis for the atmospheric contaminant mercury. The integrated modeling framework (IMF) forecasts water quality and quant...

  13. Category and Perceptual Interference in Second-Language Phoneme Learning: An Examination of English /w/-/v/ Learning by Sinhala, German, and Dutch Speakers

    ERIC Educational Resources Information Center

    Iverson, Paul; Ekanayake, Dulika; Hamann, Silke; Sennema, Anke; Evans, Bronwen G.

    2008-01-01

    The present study investigated the perception and production of English /w/ and /v/ by native speakers of Sinhala, German, and Dutch, with the aim of examining how their native language phonetic processing affected the acquisition of these phonemes. Subjects performed a battery of tests that assessed their identification accuracy for natural…

  14. Increased expression of S100A4, a metastasis-associated gene, in human colorectal adenocarcinomas.

    PubMed

    Takenaga, K; Nakanishi, H; Wada, K; Suzuki, M; Matsuzaki, O; Matsuura, A; Endo, H

    1997-12-01

    The S100A4 gene (also known as pEL98/mts1/p9Ka/18A2/42A/calvasculin /FSP1/CAPL) encoding an S100-related calcium-binding protein is implied to be involved in the invasion and metastasis of murine tumor cells. In the present study, the expression of S100A4 in human colorectal adenocarcinoma cell lines (SW837, LoVo, DLD-1, HT-29, SW480, SW620, WiDr, and Colo201) and surgically resected neoplastic tissues was examined to investigate whether S100A4 plays a role in the invasion and metastasis of human tumor cells. Northern blot analysis using total RNA isolated from the adenocarcinoma cell lines revealed that five of the eight cell lines expressed substantial amounts of S100A4 mRNA. Normal colon fibroblasts (CCD-18Co) expressed little of the RNA. Using surgically resected specimens, it seemed that the amount of S100A4 mRNA in adenomas was nearly equal to that in normal colonic mucosa, whereas adenocarcinomas expressed a significantly higher amount of the RNA than did the adjacent normal colonic mucosa. Immunohistochemical analysis using formalin-fixed paraffin-embedded surgical specimens and monoclonal anti-S100A4 antibody demonstrated that none of 12 adenoma specimens were immunopositive, whereas 8 of 18 (44%) focal carcinomas in carcinoma in adenoma specimens and 50 of 53 (94%) adenocarcinoma specimens were immunopositive. Interestingly, the incidence of immunopositive cells increased according to the depth of invasion, and nearly all of the carcinoma cells in 14 metastases in the liver were positive. These results suggest that S100A4 may be involved in the progression and the metastatic process of human colorectal neoplastic cells.

  15. 31 CFR 351.20 - What is the investment yield (interest) during the original maturity period of Series EE savings...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... email at savbonds@bpd.treas.gov, or by writing us at the following address: Bureau of the Public Debt... savbonds@bpd.treas.gov, or writing to the following address: Bureau of the Public Debt, Parkersburg,...

  16. 75 FR 82146 - Prompt Payment Interest Rate; Contract Disputes Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-29

    ...\\ per centum per annum. ADDRESSES: Comments or inquiries may be mailed to Dorothy Dicks, Reporting Team... Accounting, Bureau of the Public Debt, Parkersburg, West Virginia 26106-1328, (304) 480-5103; Dorothy...

  17. Freezing Baboon Red Blood Cells in the Original 800 ml Polyvinylchloride Plastic Bag With 40% W/V Glycerol and Storage at -80 C and Deglycerolization Using the IBM Code Blood Processor 2991

    DTIC Science & Technology

    2007-11-02

    to glycerolization. b. Materials CONSUMABLES 1. Glycerol: A. Glycerolyte 57 (6.2 M glycerol, 500 ml bottle ). Each 100 ml contains 57 g...phosphate (dried), pH 6.8 (Fenwal #4A7833) B. 6.2 M Glycerolizing Solution (500 ml bottle ). Each 100 ml contains 57.1 g glycerin, 1.6 g sodium...glycerol solution and room temperature should be within a temperature range of 20 C (68 F) to 26 C (80 F). The temperature of a bottle of glycerol

  18. Enhancing community partnerships during a public health emergency: the school-located vaccination clinics model in Kanawha County, WV during the 2009 influenza A (H1N1) pandemic.

    PubMed

    Gupta, Rahul

    2011-01-01

    Broad based community support is vital in developing a comprehensive national strategy to protect the public's health prior to, during and after a disaster such as pandemic influenza. When disaster strikes, the successful response is often dependent upon the degree of collaboration, coordination, and shared decision making occurring among a wide-ranging group of public and private stakeholders in the community. Since these preparedness and response activities must occur at a local level, the degree to which a certain community can become resilient after an event is directly dependent upon the success of the response activities. In order to protect its citizens, the Kanawha-Charleston Health Department (KCHD) led a comprehensive community based response to the 2009 Influenza A (H1N1) pandemic. By organizing a high level strategic team consisting of major community stakeholders, KCHD was able to develop broad based community support for its mitigation and countermeasure delivery strategies. The timely enhancement of the existing community partnerships enabled us to successfully conduct several response activities with local community support including school-located vaccination (SLV) clinics. We describe the process, results and challenges faced during our SLV clinics campaign which resulted in exceptionally high vaccination rates for school aged children compared to other jurisdictions across the nation. We also discuss how such partnerships can be sustained resulting in resilient communities and mention some strategies for those contemplating such partnerships in future public health emergency.

  19. 40 CFR Appendix A to Part 97 - Final Section 126 Rule: EGU Allocations, 2004-2007

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MARTIN 3943 2 868 WV GRANT TOWN 10151 ST_own 156 WV HARRISON 3944 1 1,385 WV HARRISON 3944 2 1,444 WV HARRISON 3944 3 1,505 WV JOHN E AMOS 3935 1 1,254 WV JOHN E AMOS 3935 2 1,198 WV JOHN E AMOS 3935 3...

  20. Services for Evening Students: Final Report of a Survey Conducted in the 1979 Fall Semester.

    ERIC Educational Resources Information Center

    Clovis, George A.

    An in-class survey of 1,198 students attending evening classes at Parkersburg Community College was conducted to determine: (1) the students' awareness of nine college services available during evening hours; (2) the additional services needed by evening students and the hours during which they should be provided; (3) the demographic and…

  1. 78 FR 53275 - Approval and Promulgation of Air Quality Implementation Plans; Ohio; Redesignation of the Ohio...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-29

    ... dioxide (SO 2 ), ammonia and volatile organic compounds (VOC) in the Parkersburg-Marietta area (Washington... provided ammonia and VOC emissions inventories to EPA to supplement the February 29, 2012, and April 16... 2005 NO X , SO 2 , and PM 2.5 emission inventories and 2007/2008 ammonia and VOC emission...

  2. Home Start Evaluation Study.

    ERIC Educational Resources Information Center

    High/Scope Educational Research Foundation, Ypsilanti, MI.

    Case studies of eight Home Start programs are given as the third section of an evaluation study. Communities involved are Binghamton, New York; Franklin, North Carolina; Cleveland, Ohio; Harrogate, Tennessee; Houston, Texas; Weslaco, Texas; Millville, Utah; Parkersburg, West Virginia. Although each study varies in format, each describes in detail…

  3. The Effect of microRNAs in the Regulation of Human CYP3A4: a Systematic Study using a Mathematical Model

    NASA Astrophysics Data System (ADS)

    Wei, Zhiyun; Jiang, Songshan; Zhang, Yiting; Wang, Xiaofei; Peng, Xueling; Meng, Chunjie; Liu, Yichen; Wang, Honglian; Guo, Luo; Qin, Shengying; He, Lin; Shao, Fengmin; Zhang, Lirong; Xing, Qinghe

    2014-03-01

    CYP3A4 metabolizes more than 50% of the drugs on the market. The large inter-individual differences of CYP3A4 expression may contribute to the variability of human drug responses. Post-transcriptional regulation of CYP3A4 is poorly understood, whereas transcriptional regulation has been studied much more thoroughly. In this study, we used multiple software programs to predict miRNAs that might bind to CYP3A4 and identified 112 potentially functional miRNAs. Then a luciferase reporter system was used to assess the effect of the overexpression of each potentially functional miRNA in HEK 293T cells. Fourteen miRNAs that significantly decreased reporter activity were measured in human liver samples (N = 27) as candidate miRNAs. To establish a more effective way to analyze in vivo data for miRNA candidates, the relationship between functional miRNA and target mRNA was modeled mathematically. Taking advantage of this model, we found that hsa-miR-577, hsa-miR-1, hsa-miR-532-3p and hsa-miR-627 could significantly downregulate the translation efficiency of CYP3A4 mRNA in liver. This study used in silico, in vitro and in vivo methods to progressively screen functional miRNAs for CYP3A4 and to enhance our understanding of molecular events underlying the large inter-individual differences of CYP3A4 expression in human populations.

  4. 26 CFR 1.170A-4A - Special rule for the deduction of certain charitable contributions of inventory and other property.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... of self-care (including incapacity due to old age). A person suffering from mental illness is... person is nonhospitalized or noninstitutionalized, if the person's mental illness constitutes a... an existing illness and includes care of the physical, mental, or emotional needs of the ill....

  5. 26 CFR 1.170A-4A - Special rule for the deduction of certain charitable contributions of inventory and other property.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... an existing illness and includes care of the physical, mental, or emotional needs of the ill. (D... of self-care (including incapacity due to old age). A person suffering from mental illness is... person is nonhospitalized or noninstitutionalized, if the person's mental illness constitutes...

  6. 26 CFR 1.170A-4A - Special rule for the deduction of certain charitable contributions of inventory and other property.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... an existing illness and includes care of the physical, mental, or emotional needs of the ill. (D... of self-care (including incapacity due to old age). A person suffering from mental illness is... person is nonhospitalized or noninstitutionalized, if the person's mental illness constitutes...

  7. 26 CFR 1.170A-4A - Special rule for the deduction of certain charitable contributions of inventory and other property.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... an existing illness and includes care of the physical, mental, or emotional needs of the ill. (D... of self-care (including incapacity due to old age). A person suffering from mental illness is... person is nonhospitalized or noninstitutionalized, if the person's mental illness constitutes...

  8. 26 CFR 1.170A-4A - Special rule for the deduction of certain charitable contributions of inventory and other property.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... sudden and severe personal or family crisis (such as a person who is the victim of a crime of violence or... language, cultural, or financial difficulties, a minor child who is not self-sufficient and who is not... infant. An infant is a minor child (as determined under the laws of the jurisdiction in which the...

  9. Characterization of Molecular Factors Critical to the S100A4 (A Metastasis-Associated Protein)-Dependent Increase in Motility of Breast Cancer Cells

    DTIC Science & Technology

    2005-04-01

    Cold Spring Harbor Laboratory... Cold Spring Harbor , NY 11724 REPORT DATE: April 2005 TYPE OF REPORT: Annual Summary 20060309 15 PREPARED FOR: U.S. Army Medical Research...Ph.D. 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION Cold Spring Harbor Laboratory REPORT NUMBER Cold Spring Harbor ,

  10. Commensurate and Incommensurate Phases in the System A4A'Ir 2O 9( A=Sr, Ba; A'=Cu, Zn)

    NASA Astrophysics Data System (ADS)

    Battle, Peter D.; Blake, Graeme R.; Sloan, Jeremy; Vente, Jaap F.

    1998-02-01

    The crystal structure of Sr 4CuIr 2O 9is very sensitive to the conditions of synthesis. Prolonged heating of a commensurate trigonal sample prepared at 1120°C leads to the adoption of an incommensurate structure which can be regarded as a composite of two substructures having common unit cell parameters aand bbut different parameters c1and c2. No detectable change in chemical composition accompanies the structural transition, nor do the magnetic properties of the sample change significantly. The structure of the commensurate form has been determined from neutron powder diffraction data collected at 4.5 K (space group P321, a=9.68540(3) Å, c=8.04726(6) Å). Ir 2O 9octahedral dimers and CuO 6trigonal prisms alternate in chains parallel to z, with the Sr cations located between the chains; the Cu 2+cations are disordered within the prisms. The incommensurate form has been studied by X-ray diffraction and electron microscopy ( a=9.7020(6) Å, c1=4.0069(5) Å, c2=2.6993(4) Å). The relationship between the commensurate and incommensurate unit cells is discussed ( c˜2 c1˜3 c2). No commensurate Ba 4A'Ir 2O 9phases could be prepared, but incommensurate samples having A'=Cu, Zn showed behavior similar to that of Sr 4CuIr 2O 9.

  11. The heat shock factor A4A confers salt tolerance and is regulated by oxidative stress and the mitogen-activated protein kinases MPK3 and MPK6.

    PubMed

    Pérez-Salamó, Imma; Papdi, Csaba; Rigó, Gábor; Zsigmond, Laura; Vilela, Belmiro; Lumbreras, Victoria; Nagy, István; Horváth, Balázs; Domoki, Mónika; Darula, Zsuzsa; Medzihradszky, Katalin; Bögre, László; Koncz, Csaba; Szabados, László

    2014-05-01

    Heat shock factors (HSFs) are principal regulators of plant responses to several abiotic stresses. Here, we show that estradiol-dependent induction of HSFA4A confers enhanced tolerance to salt and oxidative agents, whereas inactivation of HSFA4A results in hypersensitivity to salt stress in Arabidopsis (Arabidopsis thaliana). Estradiol induction of HSFA4A in transgenic plants decreases, while the knockout hsfa4a mutation elevates hydrogen peroxide accumulation and lipid peroxidation. Overexpression of HSFA4A alters the transcription of a large set of genes regulated by oxidative stress. In yeast (Saccharomyces cerevisiae) two-hybrid and bimolecular fluorescence complementation assays, HSFA4A shows homomeric interaction, which is reduced by alanine replacement of three conserved cysteine residues. HSFA4A interacts with mitogen-activated protein kinases MPK3 and MPK6 in yeast and plant cells. MPK3 and MPK6 phosphorylate HSFA4A in vitro on three distinct sites, serine-309 being the major phosphorylation site. Activation of the MPK3 and MPK6 mitogen-activated protein kinase pathway led to the transcriptional activation of the HEAT SHOCK PROTEIN17.6A gene. In agreement that mutation of serine-309 to alanine strongly diminished phosphorylation of HSFA4A, it also strongly reduced the transcriptional activation of HEAT SHOCK PROTEIN17.6A. These data suggest that HSFA4A is a substrate of the MPK3/MPK6 signaling and that it regulates stress responses in Arabidopsis.

  12. Final Inspection of Lactated Ringer’s Resuscitation Fluid, Intravenous Sodium Chloride Solution, and Sodium Chloride-Glucose Solution Used in the Deglycerolization of Human Red Cells Frozen With 40% W/V Glycerol, Produced by the Sterimatics EDM Reflups System and Stored for 8 Months to 2.3 Years.

    DTIC Science & Technology

    1994-09-08

    000027302908 06/28/94 P N N 121 12942 ARM2 LR1000 000027302909 06/27/94 P N N 122 12641 ARM2 LR1000 000027302910 07/06/94 P N N 123 12475 ARM2 LR1000... 12641 ARM2 LR1000 000027302910 07/06/94 P 1121 1053 PASS N/A 59 10629 ARM2 LR1000 000027302912 07/06/94 P 1123 1055 PASS N/A 235 Appendix 2.5.1

  13. 77 FR 60087 - Approval and Promulgation of Air Quality Implementation Plans; West Virginia; The 2002 Base Year...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-02

    ... 3,859 Nonroad 1 2,506 130 120 134 1,138 Onroad 86 2,667 62 44 111 1,956 Biogenic N/A 99 N/A N/A N/A...: Proposed rule. SUMMARY: EPA is proposing to approve the fine particulate matter (PM 2.5 ) 2002 base year... Parkersburg Area for the 1997 PM 2.5 National Ambient Air Quality Standard (NAAQS) SIP. EPA is proposing...

  14. 6. Photo copy of photograph, (original owned by Mary Gaudineer, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Photo copy of photograph, (original owned by Mary Gaudineer, Beckley, WV, copy at National Forest Office, Elkins, WV), Don Gaudineer, 1934. CONSTRUCTION OF FERNOW EXPERIMENTAL FOREST BUNKHOUSE AND GARAGE. (see also historic photograph WV-237-13) - Parsons Nursery, Fernow Experimental Forest Residence, South side of U.S. Route 219, Parsons, Tucker County, WV

  15. PROCEEDINGS: 1991 INTERNATIONAL CONFERENCE ON MUNICIPAL WASTE COMBUSTION - VOLUME 1. SESSIONS P, 0, 1A, 2A, 3A, 4A, 6A, 6B, 9C, AND 10B

    EPA Science Inventory

    The three-volumes document 82 presentations by authors from 15 countries at the Second International Conference on Municipal Waste Combustion (MWC) in Tampa, Florida, April 16-19, 1991. The Conference fostered the exchange of current information on research concerning MWC, ash di...

  16. Superfund Record of Decision (EPA Region 1): Fort Devens-Sudbury Training Annex (areas of contamination A4, A7, and A9), Middlesex County, MA, September 30, 1997

    SciTech Connect

    1998-01-01

    The US Army Sudbury Annex (the Annex) is a National Priorities List (NPL) site under the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA). This Record of Decision addresses past releases of contaminants to all media at area of contamination (AOC) A4-Waste Dump, and past releases to groundwater at AOC A7-Old Gravel Pit Landfill and AOC A9-Petroleum, Oil, and Lubricant (POL) Burn Area.

  17. Corrosion-Control (CC) Program SIMA (Shore Intermediate Maintenance Activity) Pilot CC (Corrosion-Control) Shop Service Test and Technical Support. Volume 2. Appendices A3, A4, A6, A8.

    DTIC Science & Technology

    1985-11-30

    protects against corrosion. The aluminum forms a galvanic coating which becomes the base for the sealing system, without which it cannot be used successfully...Formula 150 and a topcoat system. The resultant coating system protects against corrosion. The aluminum forms a galvanic coating which becomes the base...excess moisture from the surface to be protected prior to applying the corrosion-preventive compound. p Apply a thin uniform coat of corrosion-preventive

  18. Crystal structure and solvent-dependent behaviours of 3-amino-1,6-diethyl-2,5,7-trimethyl-4,4-di­phenyl-3a,4a-di­aza-4-bora-s-indacene

    PubMed Central

    Yang, Lijing; Drew, Brett; Yalagala, Ravi Shekar; Chaviwala, Rameez; Simionescu, Razvan; Lough, Alan J.; Yan, Hongbin

    2017-01-01

    In the title compound (3-amino-4,4-diphenyl-BODIPY), C28H32BN3, the central six-membered ring has a flattened sofa conformation, with one of the N atoms deviating by 0.142 (4) Å from the mean plane of the other five atoms, which have an r.m.s. deviation of 0.015 Å. The dihedral angle between the two essentially planar outer five-membered rings is 8.0 (2)°. In the crystal, mol­ecules are linked via weak N—H⋯π inter­actions, forming chains along [010]. The com­pound displays solvent-dependent behaviours in both NMR and fluorescence spectroscopy. In the 1H NMR spectra, the aliphatic resonance signals virtually coalesce in solvents such as chloro­form, di­chloro­methane and di­bromo­ethane; however, they are fully resolved in solvents such as dimethyl sulfoxide (DMSO), methanol and toluene. The excitation and fluorescence intensities in chloro­form decreased significantly over time, while in DMSO the decrease is not so profound. In toluene, the excitation and fluorescent intensities are not time-dependent. This behaviour is presumably attributed to the assembly of 3-amino-4,4-diphenyl-BODIPY in solution that leads to the formation of noncovalent structures, while in polar or aromatic solvents, the formation of these assemblies is disrupted, leading to resolution of signals in the NMR spectra. PMID:28316814

  19. The Heat Shock Factor A4A Confers Salt Tolerance and Is Regulated by Oxidative Stress and the Mitogen-Activated Protein Kinases MPK3 and MPK61[C][W][OPEN

    PubMed Central

    Pérez-Salamó, Imma; Papdi, Csaba; Rigó, Gábor; Zsigmond, Laura; Vilela, Belmiro; Lumbreras, Victoria; Nagy, István; Horváth, Balázs; Domoki, Mónika; Darula, Zsuzsa; Medzihradszky, Katalin; Bögre, László; Koncz, Csaba; Szabados, László

    2014-01-01

    Heat shock factors (HSFs) are principal regulators of plant responses to several abiotic stresses. Here, we show that estradiol-dependent induction of HSFA4A confers enhanced tolerance to salt and oxidative agents, whereas inactivation of HSFA4A results in hypersensitivity to salt stress in Arabidopsis (Arabidopsis thaliana). Estradiol induction of HSFA4A in transgenic plants decreases, while the knockout hsfa4a mutation elevates hydrogen peroxide accumulation and lipid peroxidation. Overexpression of HSFA4A alters the transcription of a large set of genes regulated by oxidative stress. In yeast (Saccharomyces cerevisiae) two-hybrid and bimolecular fluorescence complementation assays, HSFA4A shows homomeric interaction, which is reduced by alanine replacement of three conserved cysteine residues. HSFA4A interacts with mitogen-activated protein kinases MPK3 and MPK6 in yeast and plant cells. MPK3 and MPK6 phosphorylate HSFA4A in vitro on three distinct sites, serine-309 being the major phosphorylation site. Activation of the MPK3 and MPK6 mitogen-activated protein kinase pathway led to the transcriptional activation of the HEAT SHOCK PROTEIN17.6A gene. In agreement that mutation of serine-309 to alanine strongly diminished phosphorylation of HSFA4A, it also strongly reduced the transcriptional activation of HEAT SHOCK PROTEIN17.6A. These data suggest that HSFA4A is a substrate of the MPK3/MPK6 signaling and that it regulates stress responses in Arabidopsis. PMID:24676858

  20. Rejuvenation and Freezing of Additive-Preserved Red Blood Cells in the 1000 ML-600 ML Polyvinyl Chloride Freezing Bay System Stored for Up to 42 Days at 4 C Prior to Rejuvenation and Glycerolization Using 40% W/V Glycerol and Storage at -80 C, Washed in the Haemonetics Blood Processor 115, and Stored at 4 C for up to 24 Hours Prior to Transfusion

    DTIC Science & Technology

    2007-11-02

    RESEARCH LABORATORY BOSTON UNIVERSITY SCHOOL OF MEDICINE 615 ALBANY STREET BOSTON, MA 02118 $f CD 14 AUGUST 1997 Reproduction in whole or in part is...containing the red cells. 14 . Close the roller clamp, turn off the shaker and equilibrate the mixture for 2 minutes. 15. Switch the Eberbach shaker on...L. > CO 0) 3 03 ř? X CO CC gi> •■=• o$s &- 5 -ob 14 in AC O 15 < Cü ü < ü > Q. O -o o r- UJ X H O CC CD u. < 2 CQ O ü V

  1. Alterations in dopamine and serotonin uptake systems in the striatum of the weaver mutant mouse.

    PubMed

    Stotz, E H; Palacios, J M; Landwehrmeyer, B; Norton, J; Ghetti, B; Simon, J R; Triarhou, L C

    1994-01-01

    In the striatum of the homozygous weaver mutant mouse (wv/wv), dopamine content, uptake and tyrosine hydroxylase activity are decreased compared to wild-type (+/+) mice. In mice heterozygous for the weaver gene (wv/+), these dopaminergic parameters exhibit only minor reductions compared to +/+ mice. The wv/wv striatum has recently been shown to have an increase in serotonin content. In the present study, the serotonin uptake system of the weaver striatum was investigated. Synaptosomal uptake of [3H] serotonin was determined in the dorsal portion of wv/wv and +/+ striatum, and serotonin uptake sites were examined by the binding of [3H] citalopram in the striatum of wv/wv, wv/+ and +/+ mice. The dopamine uptake system was also investigated in all three genotypes via the binding of [3H] mazindol. Synaptosomal uptake of [3H] serotonin was increased by 79% in the dorsal portion of the wv/wv striatum compared to that seen in the +/+ striatum. The binding of [3H] citalopram was increased by 62% in the dorsolateral and by 111% in the dorsomedial portions of the wv/wv striatum compared to +/+. [3H] Citalopram binding in the wv/+ striatum was also higher than +/+, but this increase did not reach statistical significance. Within the wv/wv striatum, [3H] mazindol binding was almost completely absent (88-89% reduction) in the dorsal portion and severely reduced in the other striatal areas. These data support the notion that the dorsal portion of the wv/wv striatum, which has the severest reduction in dopamine uptake, is hyperinnervated by serotonin fibers.

  2. 47 CFR 76.53 - Reference points.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...: Atlantic City 39°21′32″ 74°25′53″ Burlington 40°04′21″ 74°51′47″ Camden 39°56′45″ 75°07′20″ Glen Ridge 40...′41″ 79°57′28″ Oak Hill 37°58′31″ 81°08′45″ Parkersburg 39°15′57″ 81°33′46″ Weston 39°02′19″...

  3. 47 CFR 76.53 - Reference points.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...: Atlantic City 39°21′32″ 74°25′53″ Burlington 40°04′21″ 74°51′47″ Camden 39°56′45″ 75°07′20″ Glen Ridge 40...′41″ 79°57′28″ Oak Hill 37°58′31″ 81°08′45″ Parkersburg 39°15′57″ 81°33′46″ Weston 39°02′19″...

  4. View northwest, overview of building group: chicken house (HABS No. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View northwest, overview of building group: chicken house (HABS No. WV-267-D), wash house (267-C), Albert Thacker House (267-A), coal house (267-B) (left to right in photograph) - 3249 Cyrus Road (House), Cyrus, Wayne County, WV

  5. 30. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. Photo copy of photograph, (original in Forest Service Office, Elkins, WV, 'tractor with cultivator operating'), photographer unknown, ca. 1938. VIEW SOUTHEAST, CULTIVATING SEEDLING TRANSPLANTS. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  6. 28. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. Photo copy of photograph, (original in Forest Service Office, Elkins, WV), photographer unknown, August 1939. D. A. OLIVER WITH A TRANSPLANT BOARD. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  7. 37. Photo copy of map, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    37. Photo copy of map, (original in Forest Service Office, Elkins, WV, 'Blister Rust Survey Map), 1930. PARSONS NURSERY SITE PLAN - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  8. 6. Photocopy of photograph, 1972. GRAFTON BRIDGE COLLAPSE IN AUGUST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Photocopy of photograph, 1972. GRAFTON BRIDGE COLLAPSE IN AUGUST OF 1890 (From the original collection of Miss Lois Heflin, Grafton, WV) - Northwestern Virginia Railroad, Grafton Bridge, Spanning Tygart Valley River, Grafton, Taylor County, WV

  9. 77 FR 68737 - Procurement List, Proposed Deletions

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-16

    .../Custodial, Defense Logistics Agency: Point Pleasant Depot, Defense National Stockpile Zone, 2601 Madison Avenue, Point Pleasant, WV. NPA: Prestera Center for Mental Health Services, Inc., Huntington, WV. Contracting Activity: Defense Logistics Agency Support Services-- DSS, Fort Belvoir, VA. Service...

  10. 47 CFR 97.203 - Beacon station.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Radio Astronomy Observatory, P.O. Box 2, Green Bank, WV 24944. (1) The notification must include the... Radio Astronomy Observatory at Green Bank, Pocahontas County, WV, for itself or on behalf of the...

  11. 3. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Photo copy of photograph, (original in Forest Service Office, Elkins, WV, photo #292222), photographer unknown, ca. 1935. VIEW EAST, CCC CAMP PARSONS, FOREST SERVICE TRUCK STORAGE ON LEFT, WATER TANK (DEMOLISHED), MESS HALL (DEMOLISHED). (see also historic photograph WV-237-20, WV-237-35) - Parsons Nursery, Civilian Conservation Corps Garage, South side of U.S. Route 219, Parsons, Tucker County, WV

  12. View northeast, overview of Albert Thacker building group: chicken house ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View northeast, overview of Albert Thacker building group: chicken house (HABS No. WV-267-D), wash house (267-C), privy (HABS No. WV-268-B), and house (267-A) (left to right in photograph) - 3249 Cyrus Road (House), Cyrus, Wayne County, WV

  13. Cerulean Warbler Occurrence Atlas for Military Installations

    DTIC Science & Technology

    2010-04-01

    Army Ammuniton Plant (Closed) IN NO 2009 Army IOWA ARMY AMMUNITION PLANT IA UNCERTAIN 2009 USACE J. Percy Priest Lake TN UNCERTAIN 2009 USACE J...Stonewall Jackson Lake WV UNCERTAIN 2009 USACE Summersville Lake WV no POC Army Sunflower Army Ammunition Plant KS no POC USACE Sutton Lake WV UNCERTAIN

  14. Striatal dopamine innervation and receptor density: regional effects of the weaver mutation.

    PubMed

    Pullara, J M; Marshall, J F

    1989-02-20

    Mice homozygous for the autosomal recessive gene weaver (wv) exhibit a regionally specific depletion of forebrain dopamine (DA). DA is reduced approximately 70% in the dorsal striatum of homozygotes (wv/wv) relative to heterozygous (+/wv) controls while DA content in ventral striatum is relatively unchanged. The goal of the present study was to determine the regional effects of the weaver mutation on striatal DA receptors and DA uptake sites using quantitative autoradiography. Catecholamine histofluorescence was used to examine midbrain DA-containing cell bodies. Compared to behaviorally normal (+/-) littermates, the binding of [3H]spiroperidol to D2 sites was significantly increased in the dorsal but not ventral striatum of wv/wv mice. Binding of the D1 ligand, [3H]SCH23390, was significantly decreased throughout the striatum of wv/wv mice. The binding of [3H]mazindol to DA uptake sites was dramatically reduced in all wv/wv striatal regions except the ventrolateral portion. Compared to +/- littermates, wv/wv mice had far fewer fluorescent cell bodies in the substantia nigra and a less pronounced reduction of ventral tegmental area fluorescent somata. These findings support the hypothesis that heterogeneities exist in the genetic control of the mesotelencephalic DA system. The results underscore the usefulness of the weaver mouse in the study of mesostriatal sub-systems, receptor regulation, and potentially as a model of human neuropathologies that affect distinct populations of cells in the mesotelencephalic system.

  15. Perspectives on Obesity and Its Treatment: Health Care Providers and the General Public in Rural West Virginia and Urban Baltimore

    ERIC Educational Resources Information Center

    Menez, Steven; Cheskin, Lawrence; Geller, Gail

    2013-01-01

    Objective: To determine and compare the perspectives of the general public and health care providers (HCPs) on obesity and its treatment in rural West Virginia (WV) and Baltimore, MD. Method: Surveys were completed in both locations by the general public (WV: "n" = 200; Baltimore: "n" = 171) and HCPs (WV: "n" = 25;…

  16. 76 FR 38891 - Companies Holding Certificates of Authority as Acceptable Sureties on Federal Bonds and as...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    ..., SC, SD, TN, TX, UT, VT, VA, WA, WV, WI, WY. INCORPORATED IN: Florida. ACSTAR INSURANCE COMPANY (NAIC..., RI, SC, SD, TN, TX, UT, VT, VA, WA, WV, WI, WY. INCORPORATED IN: Illinois. Aegis Security Insurance..., OR, PA, RI, SC, SD, TN, TX, UT, VT, VA, WA, WV, WI, WY. INCORPORATED IN: Pennsylvania. ALL...

  17. Vasopressin and Breast Cancer

    DTIC Science & Technology

    1996-09-01

    Denhardt’s solution (0.1% (w/v) ficoll type 400, 0.1% (w/v) polyvinylpyrrolidone, 0.1% (w/v) bovine serum albumin), 200 [tg/ml denatured salmon sperm DNA...epithelial origin. These cells possess receptors lg cfor estrogen (8, 9], progesterone [6, 10-121, calcitonin [131, antagonist 4-aminopyridine (4-AP

  18. Cerebellar cortex development in the weaver condition presents regional and age-dependent abnormalities without differences in Purkinje cells neurogenesis.

    PubMed

    Martí, Joaquín; Santa-Cruz, María C; Hervás, José P; Bayer, Shirley A; Villegas, Sandra

    2016-01-01

    Ataxias are neurological disorders associated with the degeneration of Purkinje cells (PCs). Homozygous weaver mice (wv/wv) have been proposed as a model for hereditary cerebellar ataxia because they present motor abnormalities and PC loss. To ascertain the physiopathology of the weaver condition, the development of the cerebellar cortex lobes was examined at postnatal day (P): P8, P20 and P90. Three approaches were used: 1) quantitative determination of several cerebellar features; 2) qualitative evaluation of the developmental changes occurring in the cortical lobes; and 3) autoradiographic analyses of PC generation and placement. Our results revealed a reduction in the size of the wv/wv cerebellum as a whole, confirming previous results. However, as distinguished from these reports, we observed that quantified parameters contribute differently to the abnormal growth of the wv/wv cerebellar lobes. Qualitative analysis showed anomalies in wv/wv cerebellar cytoarchitecture, depending on the age and lobe analyzed. Such abnormalities included the presence of the external granular layer after P20 and, at P90, ectopic cells located in the molecular layer following several placement patterns. Finally, we obtained autoradiographic evidence that wild-type and wv/wv PCs presented similar neurogenetic timetables, as reported. However, the innovative character of this current work lies in the fact that the neurogenetic gradients of wv/wv PCs were not modified from P8 to P90. A tendency for the accumulation of late-formed PCs in the anterior and posterior lobes was found, whereas early-generated PCs were concentrated in the central and inferior lobes. These data suggested that wv/wv PCs may migrate properly to their final destinations. The extrapolation of our results to patients affected with cerebellar ataxias suggests that all cerebellar cortex lobes are affected with several age-dependent alterations in cytoarchitectonics. We also propose that PC loss may be regionally

  19. The effect of taste masking agents on in situ gelling pectin formulations for oral sustained delivery of paracetamol and ambroxol.

    PubMed

    Miyazaki, Shozo; Kubo, Wataru; Itoh, Kunihiko; Konno, Yasuhiro; Fujiwara, Mariko; Dairaku, Masatake; Togashi, Mitsuo; Mikami, Ryozo; Attwood, David

    2005-06-13

    The aim of this study was to examine the influence of polyhydric alcohols (taste masking agents) on the rheological properties of in situ gelling pectin formulations and on the in vitro and in vivo release of paracetamol and ambroxol from these formulations. Gelation of orally administered pectin solutions containing calcium in complexed form occurred on release of calcium in the acidic environment of the stomach. Inclusion of 10% (w/v) sorbitol in 2% (w/v) pectin sols reduced the viscosity and ensured Newtonian flow properties. Xylitol and mannitol in similar concentrations were less effective in reducing viscosity; sucrose increased viscosity and caused non-Newtonian flow. The in vitro release of paracetamol from 2% (w/v) pectin gels formulated with 10% (w/v) of sorbitol, erythritol, xylitol or mannitol, and of ambroxol from 2% (w/v) pectin gels containing 10% (w/v) sorbitol, followed diffusion-controlled kinetics. Pectin gels (2%, w/v) containing sorbitol (10%, w/v) sustained the release of paracetamol in the rat stomach and bioavailabilities of approximately 90% of those from an orally administered paracetamol syrup were achieved. Sustained release of ambroxol from in situ gelling formulations was achieved with pectin concentrations of 1.5 and 1% (w/v) and a sorbitol concentration of 10% (w/v).

  20. Predictive thermal inactivation model for effects and interactions of temperature, NaCl, sodium pyrophosphate and sodium lactate on Listeria monocytogenes in ground beef

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The effects and interactions of heating temperature (60 to 73.9C), salt (0.0 to 4.5% w/v), sodium pyrophosphate (0.0 to 0.5% w/v), and sodium lactate (0.0 to 4.5% w/v) on the heat resistance of a four-strain mixture of Listeria monocytogenes in 75% lean ground beef were examined. Meat samples in st...

  1. An EWMA chart for sample range of Weibull data using weighted variance method

    NASA Astrophysics Data System (ADS)

    Atta, Abdu. M. A.; Yahaya, Sharipah Soaad Syed; Zain, Zakiyah; Ali, Hazlina

    2016-10-01

    This article proposes new EWMA chart in observing process standard deviation or dispersion with sample range of Weibull data using weighted variance method (WV). This control chart, called Weighted Variance EWMA sample range WV-EWMASR chart hereafter. The proposed WV-EWMASR chart compared with standard EWMASR of [7], skewness correction R chart (SC-R) suggested by[3]and Weighted Variance R chart (WV-R) proposed by [2], in the case of Type I and Type II errors when the data generated from Weibull distribution. Optimal parameters λ and k of the proposed WV-EWMASR and standard EWMASR are obtained via simulation using SAS program 9.4. The proposed WV-EWMASR control chart reduces to the standard EWMASR control chart of [7] when the process follow symmetric distribution. The proposed WV-EWMASR control chart has less Type I error than the standard EWMASR, SC-R and WV-R control charts, for Weibull distribution data. In case of Type II error, the proposed WV-EWMASR control chart is closer to EWMA chart with the exact limits than the standard EWMASR in [7].

  2. 7. William E. Barrett, Photographer, August 1975. LOG PONDS LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. William E. Barrett, Photographer, August 1975. LOG PONDS LOOKING WEST FROM POWERHOUSE ROOF. TRANSFORMER SHED IN FOREGROUND. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  3. 5. VIEW FROM SLIGHTLY FARTHER DOWN SERVICE ROAD, LOOKING SOUTHEAST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. VIEW FROM SLIGHTLY FARTHER DOWN SERVICE ROAD, LOOKING SOUTHEAST AT ELECTRICITY SUPPLY BUILDING, LABORATORY, AND OFFICE. - Standard Lime & Stone Quarry, County Route 27, Millville, Jefferson County, WV

  4. 12. TRANSMISSION GEARING SHOWING RELATION TO SEGMENT GEAR ON WATERWHEEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. TRANSMISSION GEARING SHOWING RELATION TO SEGMENT GEAR ON WATERWHEEL william E. Barrett, photographer, 1973 (copy negative) - Thomas Shepherd's Grist Mill, High Street Vicinity, Shepherdstown, Jefferson County, WV

  5. 30 CFR 36.3 - Consultation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., 765 Technology Drive, Triadelphia, WV 26059, to discuss with qualified MSHA personnel proposed mobile... MINING PRODUCTS APPROVAL REQUIREMENTS FOR PERMISSIBLE MOBILE DIESEL-POWERED TRANSPORTATION...

  6. Predicting sleep apnoea syndrome from heart period: a time-frequency wavelet analysis.

    PubMed

    Roche, F; Pichot, V; Sforza, E; Court-Fortune, I; Duverney, D; Costes, F; Garet, M; Barthélémy, J C

    2003-12-01

    Heart rate fluctuations are a typical finding during obstructive sleep apnoea, characterised by bradycardia during the apnoeic phase and tachycardia at the restoration of ventilation. In this study, a time-frequency domain analysis of the nocturnal heart rate variability (HRV) was evaluated as the single diagnostic marker for obstructive sleep apnoea syndrome (OSAS). The predictive accuracy of time-frequency HRV variables (wavelet (Wv) decomposition parameters from level 2 (Wv2) to level 256 (Wv256)) obtained from nocturnal electrocardiogram Holter monitoring were analysed in 147 consecutive patients aged 53.8+/-11.2 yrs referred for possible OSAS. OSAS was diagnosed in 66 patients (44.9%) according to an apnoea/hypopnoea index > or = 10. Using receiver-operating characteristic curves analysis, the most powerful predictor variable was Wv32 (W 0.758, p<0.0001), followed by Wv16 (W 0.729, p<0.0001) and Wv64 (W 0.700, p<0.0001). Classification and Regression Trees methodology generated a decision tree for OSAS prediction including all levels of Wv coefficients, from Wv2 to Wv256 with a sensitivity reaching 92.4% and a specificity of 90.1% (percentage of agreement 91.2%) with this nonparametric analysis. Time-frequency parameters calculated using wavelet transform and extracted from the nocturnal heart period analysis appeared as powerful tools for obstructive sleep apnoea syndrome diagnosis.

  7. 77 FR 76415 - Approval and Promulgation of Air Quality Implementation Plans; West Virginia; Redesignation of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-28

    ...; Redesignation of the West Virginia Portion of the Huntington-Ashland, WV-KY-OH 1997 Annual Fine Particulate... Department of Environmental Protection (WVDEP) requested that the West Virginia portion of the Huntington- Ashland, WV-KY-OH fine particulate matter (PM 2.5 ) nonattainment area (``Huntington-Ashland Area''...

  8. Passing on a Culture: West Virginia Traditional Musical Heritage and the Allegheny Echoes Programme

    ERIC Educational Resources Information Center

    Swain, Leila Ryland

    2010-01-01

    Allegheny Echoes is a summer programme of instruction and performance of music and poetry held in Marlinton, WV. The Bing Brothers learned their traditional music from the Hammons family of Pocahontas County, WV, in an informal setting of friendship and collaboration. The Hammons family was recorded in the 1970s by Alan Jabbour and others; the…

  9. 26. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. Photo copy of photograph, (original in Forest Service Office, Elkins, WV), photographer unknown, ca. 1936. VIEW NORTHWEST, GARAGE (DEMOLISHED), NURSERY MANAGER'S GARAGE AND RESIDENCE, AND PACKING BUILDING, TRANSPLANT SHEDS AND PLOW IN FOREGROUND. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  10. 29. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    29. Photo copy of photograph, (original in Forest Service Office, Elkins, WV, photo #292218, 'Planting with 10 ft. transplant board'), D. A. Oliver, 1934. VIEW EAST, FIRMING EARTH BEFORE REMOVING TRANSPLANT BOARD FROM NEWLY TRANSPLANTED SEEDLINGS. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  11. First records of Nocomis biguttatus (Hornyhead Chub) from West Virginia discovered in museum voucher specimens

    USGS Publications Warehouse

    Welsh, Stuart; Cincotta, Daniel A.; Starnes, Wayne C.

    2013-01-01

    Specimens of Nocomis biguttatus (Hornyhead Chub) from South Fork Hughes River (Little Kanawha River drainage, WV) were discovered in two museum lots at the North Carolina Museum of Natural Sciences. These accessions, collected in 1960 and 1966, represent an addition to the state fauna and are the first distribution records for this species from the Appalachian Plateau, WV

  12. 77 FR 53842 - West Virginia Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-04

    ... meetings will be held on September 17, 2012, and if necessary to complete business, also on September 21... National Forest Supervisor's Office, 200 Sycamore Street, Elkins, WV 26241. Written comments should be sent... inspect comments received at Monongahela National Forest, 200 Sycamore Street, Elkins, WV 26241....

  13. 39. Photo copy of site plan, (original in Forest Service ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    39. Photo copy of site plan, (original in Forest Service Office, Elkins, WV, 'Parsons Nursery Special Use Permit, West Virginia Department of Natural Resources'), 1969. PARSONS NURSERY SITE PLAN. CORROLATES TO PARSONS NURSERY AT TIME OF FLOOD, NOVEMBER, 1985. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  14. 75 FR 63475 - Radio Broadcasting Services; AM or FM Proposals To Change The Community of License

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-15

    ... COMMISSION Radio Broadcasting Services; AM or FM Proposals To Change The Community of License AGENCY: Federal Communications Commission. ACTION: Notice. SUMMARY: The following applicants filed AM or FM proposals to change..., From CRAIGSVILLE, WV, To WEBSTER SPRINGS, WV; ENTRAVISION HOLDINGS, LLC, Station KVVA-FM, Facility...

  15. 27. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. Photo copy of photograph, (original in Forest Service Office, Elkins, WV, photo #292219, 'Tree nurseries-transplanting-transplanting beds'), D. A. Oliver, 1934. VIEW SOUTHWEST, TRANSPLANT SHED AND MAN WITH TRANSPLANT BOARD. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  16. 23. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. Photo copy of photograph, (original in Forest Service Office, Elkins, WV, photo #248336, 'Tree nurseries-seed bed seeding machine'), D. A. Oliver, 1930. VIEW WEST, SEEDING MACHINE. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  17. 25. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. Photo copy of photograph, (original in Forest Service Office, Elkins, WV, photo #248333, 'Tree nurseries-seed bed preparation'), D. A. Oliver, 1930. VIEW SOUTHEAST, STORAGE SHED (DEMOLISHED), POPLAR SEEDLINGS, SHADED SEEDLING BEDS ON RIGHT. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  18. 14. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. Photo copy of photograph, (original in Forest Service Office, Elkins, WV, photo #333469, 'Tree nurseries-seed bed preparation'), D. A. Oliver, November 1936. VIEW NORTHEAST, ORIGINAL WASH HOUSE, GARAGE (DEMOLISHED), WORKSHOP/WAREHOUSE, AND NURSERY OFFICE. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  19. 13. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. Photo copy of photograph, (original in Forest Service Office, Elkins, WV, photo #333464, 'Tree nurseries-seed bed preparation'), D. A. Oliver, November 1936. VIEW SOUTHEAST, WORKSHOP/WAREHOUSE, ORIGINAL WASH HOUSE, NURSERY OFFICE, NURSERY MANAGER'S RESIDENCE, GARAGE (DEMOLISHED), PUMP HOUSE, AND FERNOW EXPERIMENTAL FOREST RESIDENCE. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  20. 16. Photocopy of photograph, (original in Forest Service Office, Elkins, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Photocopy of photograph, (original in Forest Service Office, Elkins, WV, photo #302581, 'Tree nurseries-Broad view, C. C. Corps project'), D. A. Oliver, May 1935. AERIAL VIEW SOUTH. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  1. 78 FR 45937 - Final Flood Hazard Determinations

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-30

    ...-based studies: Community map repository Community address Mason County, West Virginia, and Incorporated... Mason Office of the Mayor, 656 2nd Street, Mason, WV 25260. Town of New Haven Town of New Haven City Hall, 218 5th Street, New Haven, WV 25265. Unincorporated Areas of Mason County... Mason...

  2. 7. Photo copy of blue print sheet, (original in Forest ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Photo copy of blue print sheet, (original in Forest Service Office, Elkins, WV), George W. Root, draftsman, July, 1931. MAIN FLOOR PLAN SHOWING JOIST FRAMING, FOUNDATION PLAN, MAIN FLOOR PLAN, SECOND FLOOR PLAN, END ELEVATION AT SLOUGH. - Parsons Nursery, Packing Building, South side of U.S. Route 219, Parsons, Tucker County, WV

  3. View southeast, overview of building group: coal house (HABS No. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View southeast, overview of building group: coal house (HABS No. WV-267-B), large tree, Albert Thacker House (267-A), wash house (267-C), Eustace Chadwick House (268-A) (on lot to south), chicken house (267-D), and small tree (left to right in photograph) - 3249 Cyrus Road (House), Cyrus, Wayne County, WV

  4. Case Study: Effect of Handrim Diameter on Performance in a Paralympic Wheelchair Athlete

    ERIC Educational Resources Information Center

    Costa, Gabriel Brizuela; Rubio, Miguel Polo; Belloch, Salvador Llana; Soriano, Pedro Perez

    2009-01-01

    This study, with a top T-52 class athlete, determines the relationship between stroke frequency (SF) and push time (PT) and wheelchair velocity (Wv) using different handrim diameters (HD) and the effect of different HDs on the athlete's heart rate (HR) and blood lactate (LACT) at competition speeds. Wv shows a linear-direct relationship with SF…

  5. 4. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Photo copy of photograph, (original in Forest Service Office, Elkins, WV, photo #302582, 'Insect/disease control'), D. A. Oliver, May 1935. VIEW NORTHEAST. - Parsons Nursery, Packing Building, South side of U.S. Route 219, Parsons, Tucker County, WV

  6. The effect of bioleaching on sewage sludge pyrolysis.

    PubMed

    Chen, Zhihua; Hu, Mian; Cui, Baihui; Liu, Shiming; Guo, Dabin; Xiao, Bo

    2016-02-01

    The effects of bioleaching on sewage sludge pyrolysis were studied. Sewage sludge was treated by bioleaching with solid concentrations of 6% (w/v), 8% (w/v), 10% (w/v). Results showed that bioleaching treatment could modify the physicochemical properties of sewage sludge and enhance the metals removal. The optimum removal efficiencies of heavy metals were achieved with solid concentration of 6% (w/v) bioleaching treatment: Cu, 73.08%; Zn, 78.67%; Pb, 24.65%; Cd, 79.46%. The characterization results of thermogravimetric analysis (TGA) showed that the bioleached sewage sludge with a 6% (w/v) solid concentration treatment was the easiest to decompose. Pyrolytic experiments of bioleached sewage sludge were performed in a laboratory-scale fixed bed reactor. Results indicated that bioleaching treatment greatly influenced the product yields and gas composition.

  7. Space-borne observations of aerosol - cloud relations for cloud systems of different heights

    NASA Astrophysics Data System (ADS)

    Stathopoulos, S.; Georgoulias, A. K.; Kourtidis, K.

    2017-01-01

    Here, we examine the aerosol - cloud relations over three major urban clusters of China, representative of three different climatic regimes, under different water vapor conditions and cloud heights, using Aerosol Optical Depth at 550 nm (AOD), Cloud Fraction (CC), Cloud Optical Depth (COD), Water Vapor (WV) and Cloud Top Pressure (CTP) data from the MODIS instrument. Over all regions and for all seasons, CC is found to increase with increasing AOD, WV and cloud height. Aerosols, at low WV environments and under constant CTP, have less impact on CC than at high WV environments. Furthermore, AOD has a varying influence on COD depending on CTP. Finally, COD is found to increase with height for low and middle height clouds, and with increasing AOD, especially at low AOD. Our results demonstrate that the role of WV in the observed satellite-based aerosol - cloud relations is significant for all cloud heights.

  8. Increasing alcohol yield by selected yeast fermentation of sweet sorghum. I. Evaluation of yeast strains for ethanol production

    SciTech Connect

    de Mancilha, I.M.; Pearson, A.M.; Waller, J.; Hogaboam, G.J.

    1984-01-01

    A study was conducted for the purpose of evaluating and selecting yeast strains for their ability to produce ethanol using sweet sorghum juice as the substrate. Stalks of sweet sorghum were obtained by cutting off the tops and stripping away the leaves. Fermentation media were prepared by diluting or adding dextrose to the sorghum juice to give a sugar concentration of either 10% (w/v) or 20% (w/v). All yeast strains were first tested in 10% (w/v) total sugar medium. Those strains showing more than 90% sugar conversion efficiency were further tested in 20% (w/v) total sugar medium. Active cultures for inoculation were prepared by growing the yeast strains on the fermentation medium (10% (w/v) total sugar) for 24 h. Then the cultures were added to the fermentation media at a rate of 2%.

  9. West Virginia Full-Scale Solar Energy Team Growing the Mountain State Energy Economy by Reaching our Full Solar Potential

    SciTech Connect

    Inc., Geostellar,

    2012-02-15

    The WV Team will design and demonstrate a complete set of systems and processes for automating the identification of residential and light commercial solar opportunities, evaluating project finance options, securing permits and arranging for interconnections to the utility grid. These operations will be supported by the predictive qualification and feasibility of residential and light commercial solar energy opportunities in WV based on the current solar LCOE and potential state-wide policy initiatives. Through the WV Department of Commerce and its Division of Energy, the WV Team will coordinate with all 55 county development authorities in West Virginia, as well as a number of local and municipal authorities responsible for zoning and building code ordinances to support and implement a statewide, integrated set of regulations and administrative services. Additionally the WV Team will work with the State administration and legislature to develop incentives that will promote the financing of residential and light commercial solar projects.

  10. Optimization of γ-amino butyric acid production in a newly isolated Lactobacillus brevis.

    PubMed

    Binh, Tran Thi Thanh; Ju, Wan-Taek; Jung, Woo-Jin; Park, Ro-Dong

    2014-01-01

    An isolate from kimchi, identified as Lactobacillus brevis, accumulated γ-aminobutyric acid (GABA), a major inhibitory neurotransmitter, in the culture medium. Optimal culture conditions for growth of L. brevis and production of GABA were 6 % (w/v) l-glutamic acid, 4 % (w/v) maltose, 2 % (w/v) yeast extract, 1 % (w/v) NaCl, 1 % (w/v) CaCl2, 2 g Tween 80/l, and 0.02 mM pyridoxal 5′-phosphate at initial pH 5.25 and 37 °C. GABA reached 44.4 g/l after 72 h cultivation with a conversion rate 99.7 %, based on the amount (6 %) of l-glutamic acid added. GABA was purified using ion exchange column chromatography with 70 % recovery and 97 % purity.

  11. Reliability and validity of heart rate variability threshold assessment during an incremental shuttle-walk test in middle-aged and older adults

    PubMed Central

    Dourado, V.Z.; Guerra, R.L.F.

    2013-01-01

    Studies on the assessment of heart rate variability threshold (HRVT) during walking are scarce. We determined the reliability and validity of HRVT assessment during the incremental shuttle walk test (ISWT) in healthy subjects. Thirty-one participants aged 57 ± 9 years (17 females) performed 3 ISWTs. During the 1st and 2nd ISWTs, instantaneous heart rate variability was calculated every 30 s and HRVT was measured. Walking velocity at HRVT in these tests (WV-HRVT1 and WV-HRVT2) was registered. During the 3rd ISWT, physiological responses were assessed. The ventilatory equivalents were used to determine ventilatory threshold (VT) and the WV at VT (WV-VT) was recorded. The difference between WV-HRVT1 and WV-HRVT2 was not statistically significant (median and interquartile range = 4.8; 4.8 to 5.4 vs 4.8; 4.2 to 5.4 km/h); the correlation between WV-HRVT1 and WV-HRVT2 was significant (r = 0.84); the intraclass correlation coefficient was high (0.92; 0.82 to 0.96), and the agreement was acceptable (-0.08 km/h; -0.92 to 0.87). The difference between WV-VT and WV-HRVT2 was not statistically significant (4.8; 4.8 to 5.4 vs 4.8; 4.2 to 5.4 km/h) and the agreement was acceptable (0.04 km/h; -1.28 to 1.36). HRVT assessment during walking is a reliable measure and permits the estimation of VT in adults. We suggest the use of the ISWT for the assessment of exercise capacity in middle-aged and older adults. PMID:23369974

  12. Identification of a novel multiple kinase inhibitor with potent antiviral activity against influenza virus by reducing viral polymerase activity

    SciTech Connect

    Sasaki, Yutaka; Kakisaka, Michinori; Chutiwitoonchai, Nopporn; Tajima, Shigeru; Hikono, Hirokazu; Saito, Takehiko; Aida, Yoko

    2014-07-18

    Highlights: • Screening of 50,000 compounds and subsequent lead optimization identified WV970. • WV970 has antiviral effects against influenza A, B and highly pathogenic viral strains. • WV970 inhibits viral genome replication and transcription. • A target database search suggests that WV970 may bind to a number of kinases. • KINOMEscan screening revealed that WV970 has inhibitory effects on 15 kinases. - Abstract: Neuraminidase inhibitors are the only currently available influenza treatment, although resistant viruses to these drugs have already been reported. Thus, new antiviral drugs with novel mechanisms of action are urgently required. In this study, we identified a novel antiviral compound, WV970, through cell-based screening of a 50,000 compound library and subsequent lead optimization. This compound exhibited potent antiviral activity with nanomolar IC{sub 50} values against both influenza A and B viruses but not non-influenza RNA viruses. Time-of-addition and indirect immunofluorescence assays indicated that WV970 acted at an early stage of the influenza life cycle, but likely after nuclear entry of viral ribonucleoprotein (vRNP). Further analyses of viral RNA expression and viral polymerase activity indicated that WV970 inhibited vRNP-mediated viral genome replication and transcription. Finally, structure-based virtual screening and comprehensive human kinome screening were used to demonstrate that WV970 acts as a multiple kinase inhibitor, many of which are associated with influenza virus replication. Collectively, these results strongly suggest that WV970 is a promising anti-influenza drug candidate and that several kinases associated with viral replication are promising drug targets.

  13. Soluble copolymer of wasp venom with human albumin for venom immunotherapy.

    PubMed

    Gewurz, A; Grammer, L C; Shaughnessy, M A; Patterson, R

    1986-03-01

    Polymerization of allergens decreases allergenicity while retaining immunogenicity, as we have demonstrated for ragweed, grass, and tree pollens. We have also polymerized bee venom with human albumin to form soluble, high-molecular-weight copolymers that are immunogenic in rabbits. We now have prepared a soluble wasp venom-albumin polymer (WVAP), molecular weight greater than or equal to 240,000 daltons, by glutaraldehyde treatment and Sephacryl S-300 column fractionation. Rabbits immunized with WVAP produced IgG to both WVAP and wasp venom (WV), as measured by ELISA. IgG against WVAP was totally inhibitable by a mixture of WV and albumin, demonstrating both retention of native antigens and absence of new antigenic determinants in WVAP. IgG against WV in serum from patients receiving maintenance doses of WV immunotherapy was inhibited by WVAP. In summary, we have synthesized a soluble, high-molecular-weight copolymer of WV that retains the immunogenicity of native WV, contains no new antigenic determinants, and has potential value in the treatment of patients with WV anaphylaxis.

  14. Factors affecting mycelial biomass and exopolysaccharide production in submerged cultivation of Antrodia cinnamomea using complex media.

    PubMed

    Lin, En-Shyh; Chen, Yueh-Hsiang

    2007-09-01

    Submerged cultures were used to identify growth-limiting nutrients by Antrodia cinnamomea strains. The mycelial biomass and EPS production by A. cinnamomea BCRC 35396 were markedly higher than other A. cinnamomea strains. A relatively high C/N ratio was favorable for both the mycelial growth (5.41 g/l) and EPS production (0.55 g/l); the optimum ratio was 40. The glucose was available utilized preferentially for mycelial growth, rather than for EPS production. Flushing the culture medium with nitrogen had a stimulating effect on both mycelial growth and EPS production. In addition, peptone, yeast extract and malt extract appeared to be important and significant component for EPS production. Phosphate ion, magnesium ion and thiamine were probably not essential for mycelial growth. By optimizing the effects of additional nutrition, the results showed that 5% (w/v) glucose, 0.8% (w/v) peptone, 0.8% (w/v) yeast extract, 0.8% (w/v) malt extract, 0.03% (w/v) KH2PO4, 0.1% (w/v) MgSO4 .7H2O and 0.1% (w/v) thiamine could lead to the maximum production of EPS (1.36 g/l).

  15. Cellulase and Xylanase Production by Penicillium echinulatum in Submerged Media Containing Cellulose Amended with Sorbitol

    PubMed Central

    Todero Ritter, Carla Eliana; Camassola, Marli; Zampieri, Denise; Silveira, Mauricio Moura; Dillon, Aldo José Pinheiro

    2013-01-01

    The present work investigated the use of sorbitol as a soluble carbon source, in association with cellulose, to produce cellulases and xylanases in submerged cultures of Penicillium echinulatum 9A02S1. Because cellulose is an insoluble carbon source, in cellulase production, there are some problems with rheology and oxygen transfer. The submerged fermentations containing media composed of 0, 0.25, 0.5, 0.75, and 1% (w/v) sorbitol and cellulose that were added at different times during the cultivation; 0.2% (w/v) soy bran; 0.1% (w/v) wheat bran; and a solution of salts. The highest filter paper activity (FPA) (1.95  ±  0.04 IU·mL−1) was obtained on the seventh day in the medium containing 0.5% (w/v) sorbitol and 0.5% (w/v) cellulose added 24 h after the start of cultivation. However, the CMCases showed an activity peak on the sixth day (9.99 ± 0.75 IU·mL−1) in the medium containing 0.75% (w/v) sorbitol and 0.75% (w/v) cellulose added after 12 h of cultivation. The xylanases showed the highest activity in the medium with 0.75% (w/v) sorbitol and 0.25% (w/v) cellulose added 36 h after the start of cultivation. This strategy enables the reduction of the cellulose concentration, which in high concentrations can cause rheological and oxygen transfer problems. PMID:24058733

  16. Metallothionein-mediated neuroprotection in genetically engineered mouse models of Parkinson's disease

    PubMed Central

    Ebadi, Manuchair; Brown-Borg, Holly; Refaey, Hesham El; Singh, Brij B.; Garrett, Scott; Shavali, Shaik; Sharma, Sushil K.

    2013-01-01

    Parkinson's disease is characterized by a progressive loss of dopaminergic neurons in the substantia nigra zona compacta, and in other sub-cortical nuclei associated with a widespread occurrence of Lewy bodies. The cause of cell death in Parkinson's disease is still poorly understood, but a defect in mitochondrial oxidative phosphorylation and enhanced oxidative and nitrative stresses have been proposed. We have studied controlwt (C57B1/6), metallothionein transgenic (MTtrans), metallothionein double gene knock (MTdko), α-synuclein knock out (α-synko), α-synuclein–metallothionein triple knock out (α-syn–MTtko), weaver mutant (wv/wv) mice, and Ames dwarf mice to examine the role of peroxynitrite in the etiopathogenesis of Parkinson's disease and aging. Although MTdko mice were genetically susceptible to 1, methyl, 4-phenyl, 1,2,3,6-tetrahydropyridine (MPTP) Parkinsonism, they did not exhibit any overt clinical symptoms of neurodegeneration and gross neuropathological changes as observed in wv/wv mice. Progressive neurodegenerative changes were associated with typical Parkinsonism in wv/wv mice. Neurodegenerative changes in wv/wv mice were observed primarily in the striatum, hippocampus and cerebellum. Various hallmarks of apoptosis including caspase-3, TNFα, NFκB, metallothioneins (MT-1, 2) and complex-1 nitration were increased; whereas glutathione, complex-1, ATP, and Ser(40)-phosphorylation of tyrosine hydroxylase, and striatal 18F-DOPA uptake were reduced in wv/wv mice as compared to other experimental genotypes. Striatal neurons of wv/wv mice exhibited age-dependent increase in dense cored intra-neuronal inclusions, cellular aggregation, proto-oncogenes (c-fos, c-jun, caspase-3, and GAPDH) induction, inter-nucleosomal DNA fragmentation, and neuro-apoptosis. MTtrans and α-Synko mice were genetically resistant to MPTP-Parkinsonism and Ames dwarf mice possessed significantly higher concentrations of striatal coenzyme Q10 and metallothioneins (MT 1, 2

  17. 21. Photocopy of photograph, photographer: The Thomas Studios, Huntington, date ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. Photocopy of photograph, photographer: The Thomas Studios, Huntington, date unknown. View northwest, rear elevation completed building. - Veterans Administration Medical Center, Building No. 1, Spring Valley Drive, Huntington, Cabell County, WV

  18. 19. Photocopy of photograph, photographer: The Thomas Studios, Huntington, May ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. Photocopy of photograph, photographer: The Thomas Studios, Huntington, May 4, 1932. View east front elevation during construction. - Veterans Administration Medical Center, Building No. 1, Spring Valley Drive, Huntington, Cabell County, WV

  19. 4. GENERAL VIEW LOOKING WEST AT MACHINE SHOP IN KEYSER. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. GENERAL VIEW LOOKING WEST AT MACHINE SHOP IN KEYSER. OLD TRAIN SHED TO RIGHT OF MACHINE SHOP. - Baltimore & Ohio Railroad, Keyser Machine Shop, State Route 46 Northwest of Spring Street, Keyser, Mineral County, WV

  20. 75 FR 71462 - Investigations Regarding Certifications of Eligibility To Apply for Worker Adjustment Assistance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-23

    ... Cranberry Resources, LLC Beckley, WV........ 11/01/10 10/25/10 (Company). 74806 Cranberry Hardwoods, Beckley..., TN.. 11/05/10 10/25/10 Company (Company). 74826 Cranberry Lumber Company Newport, OH...........

  1. 8. Environmental view facing northwest showing pond in relationship to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Environmental view facing northwest showing pond in relationship to house - John Bly House, East side of County Road 857, just north of intersection with Quarry Run Road, Cheat Neck, Monongalia County, WV

  2. 7. William E. Barrett, Photographer, 1974. SKEWED VIEW SHOWING CHEAT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. William E. Barrett, Photographer, 1974. SKEWED VIEW SHOWING CHEAT RIVER VALLEY, REMAINS OF 1887 PIER AND c. 1900 MASONRY ARCHES. - Baltimore & Ohio Railroad, Tray Run Viaduct, Spanning Tray Run, Rowlesburg, Preston County, WV

  3. Environmental perspective facing south showing chicken house, tractor shed, and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Environmental perspective facing south showing chicken house, tractor shed, and homestead - Norris Farm, .5 mile west of County Road 857 & .25 mile east of County Road 88/1, Cheat Neck, Monongalia County, WV

  4. North and east elevations of tractor shed facing southwest ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    North and east elevations of tractor shed facing southwest - Norris Farm, Tractor Shed-Combine Barn, .5 mile west of County Road 857 & .25 mile east of County Road 88/1, Cheat Neck, Monongalia County, WV

  5. South and west elevations of tractor shed facing northeast ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    South and west elevations of tractor shed facing northeast - Norris Farm, Tractor Shed-Combine Barn, .5 mile west of County Road 857 & .25 mile east of County Road 88/1, Cheat Neck, Monongalia County, WV

  6. 34. View of pier 3, showing supporting main anchor arm ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    34. View of pier 3, showing supporting main anchor arm and cantilever arm spans, as seen from shore near pier 4, looking north - Williamstown-Marietta Bridge, Spanning Ohio River between Williamstown & Marietta, Williamstown, Wood County, WV

  7. 22. View showing main anchor arm, as viewed from main ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. View showing main anchor arm, as viewed from main cantilever arm looking south. Note upper chord eyebar arrangement. - Williamstown-Marietta Bridge, Spanning Ohio River between Williamstown & Marietta, Williamstown, Wood County, WV

  8. 75 FR 52978 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-30

    ... of Alcohol, Tobacco, Firearms and Explosives Agency Information Collection Activities: Proposed... Federal firearms license. The Department of Justice (DOJ), Bureau of Alcohol, Tobacco, Firearms and... Firearms Licensing Center, 244 Needy Road, Martinsburg, WV 20226. Written comments and suggestions from...

  9. 75 FR 52977 - Agency Information Collection Activities: Proposed Collection; Comments Requested

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-30

    ... of Alcohol, Tobacco, Firearms and Explosives Agency Information Collection Activities: Proposed... Alcohol, Tobacco, Firearms and Explosives (ATF), will be submitting the following information collection... Desiree Winger, Firearms and Explosives Imports Branch, 244 Needy Road, Martinsburg, WV 25405....

  10. 78 FR 76322 - Agency Information Collection Activities: Proposed Collection; Comments Requested: FFL Out-of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-17

    ... of Alcohol, Tobacco, Firearms and Explosives Agency Information Collection Activities: Proposed... Justice (DOJ), Bureau of Alcohol, Tobacco, Firearms and Explosives (ATF), will submit the following... Firearms Licensing Center, 244 Needy Road, Martinsburg, WV 25405. Written comments and suggestions from...

  11. 77 FR 4827 - Agency Information Collection Activities; Proposed Collection; Comments Requested: FFL Out-of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-31

    ... of Alcohol, Tobacco, Firearms and Explosives Agency Information Collection Activities; Proposed... Collection. The Department of Justice (DOJ), Bureau of Alcohol, Tobacco, Firearms and Explosives (ATF), will...-4647, Acting Chief, Federal Firearms Licensing Center, 244 Needy Road, Martinsburg, WV 25405....

  12. 32. Credit JTL. Exterior transformer bank; note lightning arrestors removed ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    32. Credit JTL. Exterior transformer bank; note lightning arrestors removed from pad and smaller arrestors installed on transformers and in area near air switches. - Dam No. 4 Hydroelectric Plant, Potomac River, Martinsburg, Berkeley County, WV

  13. 13. View South, showing the remaining pier footings for the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. View South, showing the remaining pier footings for the steam engine water tower for the Chesapeake and Ohio Railroad. - Cotton Hill Station Bridge, Spanning New River at State Route 16, Cotton Hill, Fayette County, WV

  14. 9. (5 X 7 enlargement from 4 X 5 negative) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. (5 X 7 enlargement from 4 X 5 negative) FIRST FLOOR, WINDOW MOLDING ON SOUTH WALL LOOKING SOUTH - Sites Homestead, Monongahela National Forest (Tract 390) East of Route 28, Seneca Rocks, Pendleton County, WV

  15. 4. EASTBOUND VIEW. NORTH TRACK WAITING STATION ON LEFT. STATION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. EASTBOUND VIEW. NORTH TRACK WAITING STATION ON LEFT. STATION ON RIGHT. NOTE TUNNEL IN BACKGROUND. - Baltimore & Ohio Railroad, Harpers Ferry Station, Potomac Street, Harpers Ferry, Jefferson County, WV

  16. 13. VIEW FROM POTOMAC RIVER BRIDGE PLATFORM WEST TOWARDS STATION. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. VIEW FROM POTOMAC RIVER BRIDGE PLATFORM WEST TOWARDS STATION. HARPERS FERRY DEPOT IS ON LEFT, NORTH TRACK WAITING STATION IS ON RIGHT. - Baltimore & Ohio Railroad, Harpers Ferry Station, Potomac Street, Harpers Ferry, Jefferson County, WV

  17. View of Harpers Ferry, station behind trees in lower right. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of Harpers Ferry, station behind trees in lower right. Taken from Maryland Heights Ridge from the east. - Baltimore & Ohio Railroad, Harpers Ferry Station, Potomac Street, Harpers Ferry, Jefferson County, WV

  18. View of Harpers Ferry, station to right at end of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of Harpers Ferry, station to right at end of CSX Railroad Bridge. Taken from Maryland Heights Ridge from the east. - Baltimore & Ohio Railroad, Harpers Ferry Station, Potomac Street, Harpers Ferry, Jefferson County, WV

  19. 1. Photocopied 1974 from Album of Baltimore and Ohio Railroad, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Photocopied 1974 from Album of Baltimore and Ohio Railroad, Witteman Brothers, Smithsonian Institution, 1882. WOODCUT ILLUSTRATION OF TRESTLE WORK, CHEAT RIVER GORGE. - Baltimore & Ohio Railroad, Tray Run Viaduct, Spanning Tray Run, Rowlesburg, Preston County, WV

  20. 2. Photocopied 1972 from Ecole imperiale des ponts et chaussees, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Photocopied 1972 from Ecole imperiale des ponts et chaussees, Collection de Dessins Distribues aus eleves, (n.d. and no place), ORIGINAL FINK TRUSS BRIDGE, 1852. - Baltimore & Ohio Railroad, Fairmont Bridge, Spanning Monongahela River, Fairmont, Marion County, WV

  1. View west, barn, east elevation Woods Homestead, Barn, County ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View west, barn, east elevation - Woods Homestead, Barn, County Route 12 on north side of North Fork of Hughes River, 2.2 miles north & east of Goose Run Road intersection, Harrisville, Ritchie County, WV

  2. View south, barn, north elevation Woods Homestead, Barn, County ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View south, barn, north elevation - Woods Homestead, Barn, County Route 12 on north side of North Fork of Hughes River, 2.2 miles north & east of Goose Run Road intersection, Harrisville, Ritchie County, WV

  3. View west, interior, detail of joinery Woods Homestead, Barn, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View west, interior, detail of joinery - Woods Homestead, Barn, County Route 12 on north side of North Fork of Hughes River, 2.2 miles north & east of Goose Run Road intersection, Harrisville, Ritchie County, WV

  4. View southeast, general view, barn at left Woods Homestead, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View southeast, general view, barn at left - Woods Homestead, County Route 12 on north side of North Fork of Hughes River, 2.2 miles north & east of Goose Run Road intersection, Harrisville, Ritchie County, WV

  5. View north, barn, south elevation Woods Homestead, Barn, County ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View north, barn, south elevation - Woods Homestead, Barn, County Route 12 on north side of North Fork of Hughes River, 2.2 miles north & east of Goose Run Road intersection, Harrisville, Ritchie County, WV

  6. View east, barn, west elevation Woods Homestead, Barn, County ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View east, barn, west elevation - Woods Homestead, Barn, County Route 12 on north side of North Fork of Hughes River, 2.2 miles north & east of Goose Run Road intersection, Harrisville, Ritchie County, WV

  7. 27. Credit JTL. Second floor, detail of Eureka horizontal wheat ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. Credit JTL. Second floor, detail of Eureka horizontal wheat scourer, by S. Howes Co., (Silver Creek, NY). After leaving scourer, grain passed directly to the Midget Marvel Machine. - Bunker Hill Mill, County Route 26, Bunker Hill, Berkeley County, WV

  8. 78 FR 2394 - Columbia Gas Transmission, LLC; Notice of Request Under Blanket Authorization

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-11

    ... its Seneca Compressor Station located in Pendleton County, WV. Columbia also seeks authorization to abandon by removal an existing 6,200 horsepower compressor unit at its Seneca Compressor Station....

  9. 27. William E. Barrett, Photographer, August 1975. TRIMMER SAWS LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. William E. Barrett, Photographer, August 1975. TRIMMER SAWS LOOKING BACK FROM SORTING DOCK. SAW BLADES ARE HIDDEN BY HINGED PARTITION. SPIRAL ROLLERS CARRY BOARDS FROM SAWS TO HANDLES. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  10. 8. VIEW EAST, CIVILIAN CONSERVATION CORPS CAMP PARSONS GARAGE, MODERN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. VIEW EAST, CIVILIAN CONSERVATION CORPS CAMP PARSONS GARAGE, MODERN SHED, CIVILIAN CONSERVATION CORPS CAMP PARSONS BLACKSMITH SHOP, AND MODERN SHED. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  11. Conformity Adequacy Review: Region 3

    EPA Pesticide Factsheets

    Resources are for air quality and transportation government and community leaders. Information on the adequacy/inadequacy of state implementation plans (SIPs) in EPA Region 3 (DE, DC, MD, PA, VA, WV) is provided here.

  12. 1. Photocopy of engraved flyer (from the collection of Dr. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Photocopy of engraved flyer (from the collection of Dr. Margaret Ballard, Union, West Virginia) H. Bartsch, engraver late 19th century AERIAL VIEW OF TOWN AND ADVERTISING SLOGANS - Red Sulphur Springs, Route 12, Salt Sulphur Springs, Monroe County, WV

  13. 31 CFR 353.1 - Official agencies.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Service's Web site at http://www.treasurydirect.gov. Communications concerning transactions and requests..., WV 26106-7012; or (2) The Federal Reserve Bank of Minneapolis, Treasury Retail Securities Site,...

  14. 3. Photocopy of 1977 photograph (original negative lost) 2307 (AT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Photocopy of 1977 photograph (original negative lost) 2307 (AT EXTREME RIGHT) THROUGH 2319 CHAPLINE STREET - Chapline Street Row Historic District, 2301-2319 Chapline Street, Wheeling, Ohio County, WV

  15. 9. LOOKING EAST AND TOWARD SMALL TANK, 60FOOT SQUARE BASIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. LOOKING EAST AND TOWARD SMALL TANK, 60-FOOT SQUARE BASIN (ON EXTREME RIGHT) AND 125-FOOT BASIN (IN BACKGROUND). - Standard Lime & Stone Quarry, Tanks & Settling Basins, County Route 27, Millville, Jefferson County, WV

  16. 78 FR 16035 - Qualification of Drivers; Exemption Applications; Vision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-13

    ... renewable two-year period. They are: Richard D. Carlson (MN) Robert P. Conrad, Sr. (MD) Donald P. Dodson, Jr. (WV) James A. Stoudt (PA) Ralph A. Thompson (KY) The exemptions are extended subject to the...

  17. TOWER, WEST ELEVATION, SHOWING CONNECTION PIPES FOR TURNOUTS 22 (FOREGROUND) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    TOWER, WEST ELEVATION, SHOWING CONNECTION PIPES FOR TURNOUTS 22 (FOREGROUND) AND 24. NOTE “LAZY JACK” TEMPERATURE COMPENSATOR IN FOREGROUND. - Baltimore & Ohio Railroad, Z Tower, State Route 46, Keyser, Mineral County, WV

  18. 77 FR 74734 - Qualification of Drivers; Exemption Applications; Vision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-17

    .... Isenhart (WV) Damir Kocijan (FL) Robert T. Lantry (MA) John W. Laskey (NH) Kenneth Liuzza (LA) Samson B... C. Schmude (WI) Dennis J. Smith (CO) David C. Stitt (KS) Kevin L. Truxell (FL) Bruce A. Walker...

  19. 12. Credit WS. Basement of Mill, showing wooden flywheels to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. Credit WS. Basement of Mill, showing wooden flywheels to water wheel and Fairbanks. Morse 20 hp, 350 rpm diesel engine, patented April 20, 1920. - Bunker Hill Mill, County Route 26, Bunker Hill, Berkeley County, WV

  20. 14. INTERIOR VIEW OF FIREPLACE AND SURROUNDING WALL IN FIRST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. INTERIOR VIEW OF FIREPLACE AND SURROUNDING WALL IN FIRST FLOOR, NORTHWEST PARLOR, NORTH WALL, WITH SCALE (NOTE WALL STENCILING) - George W. Eckhart House, 810 Main Street, Wheeling, Ohio County, WV

  1. 2. DETAIL OF INVERTED MINE CARS, LOOKING SOUTHWEST; NOTE SHORT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. DETAIL OF INVERTED MINE CARS, LOOKING SOUTHWEST; NOTE SHORT WHEEL BASE NEEDED FOR DUMPING CONTENTS - Nuttallburg Mine Complex, Main Mine, North side of New River, 2.7 miles upstream from Fayette Landing, Lookout, Fayette County, WV

  2. 16. William E. Barrett, Photographer, August 1975. CENTER MILL (RIGHTHANDED) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. William E. Barrett, Photographer, August 1975. CENTER MILL (RIGHT-HANDED) WITH CARRIAGE TRACKS AND BANDSAW HOUSING INTACT. LOG CLAMPS ON THIS CARRIAGE WERE PNEUMATICALLY OPERATED. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  3. 21. William E. Barrett, Photographer, August 1975. EDGER SAWS FOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. William E. Barrett, Photographer, August 1975. EDGER SAWS FOR RIPPING BOARD TO VARIOUS WIDTHS. BLADES VISIBLE BEHIND ROLLERS. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  4. 8. William E. Barrett, Photographer, August 1975. LOG DOCK AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. William E. Barrett, Photographer, August 1975. LOG DOCK AND PARTIALLY DEMOLISHED JACKSLIP USED FOR HAULING LOGS UP TO SAWMILL. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  5. 13. William E. Barrett, Photographer, August 1975. INTERIOR OF SAWMILL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. William E. Barrett, Photographer, August 1975. INTERIOR OF SAWMILL LOOKING EAST. LOGS FOR CENTER MILL ROLLED RIGHT TO CONVEYOR WHICH CARRIED THEM TO SECOND SLOPING DECK. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  6. 11. William E. Barrett, Photographer, August 1975. INTERIOR OF SAWMILL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. William E. Barrett, Photographer, August 1975. INTERIOR OF SAWMILL SHOWING STEAM CYLINDER AND PISTON ROD OF LEFT-HAND MILL (LEFT) AND HOUSING OF CENTER BANDSAW. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  7. 20. William E. Barrett, Photographer, August 1975. INTERIOR OF SAWMILL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. William E. Barrett, Photographer, August 1975. INTERIOR OF SAWMILL LOOKING EAST. EDGER SAW AT LOWER LEFT, TRIMMERS AT RIGHT. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  8. 19. William E. Barrett, Photographer, August 1975. LOWER BAND SAW ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. William E. Barrett, Photographer, August 1975. LOWER BAND SAW PULLEYS OF RIGHT-HAND MILL. DRIVE PULLEY IN BELOW TENSION PULLEY. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  9. 6. William E. Barrett, Photographer, 1975. GENERAL VIEW LOOKING EAST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. William E. Barrett, Photographer, 1975. GENERAL VIEW LOOKING EAST. SAWMILL ON RIGHT. SECONDARY WOODWORKING SHOPS ON LEFT PARTIALLY DEMOLISHED. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  10. 1. LOOKING NORTH, SHOWING IODINE SPRING (FOREGROUND), SALT SULPHUR SPRING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. LOOKING NORTH, SHOWING IODINE SPRING (FOREGROUND), SALT SULPHUR SPRING (LEFT BACKGROUND), AND TWIN COTTAGES (UPPER RIGHT) (4 x 5 negative; 5 x 7 print) - Salt Sulpher Springs, U.S. Route 219, Salt Sulphur Springs, Monroe County, WV

  11. 45. INTERIOR VIEW SHOWING CONVEYOR BELT SYSTEM WHICH CONVEY THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    45. INTERIOR VIEW SHOWING CONVEYOR BELT SYSTEM WHICH CONVEY THE HARDENED NAILS TO THE DRAWBACK TUBE FOR TEMPERING; MOTIONED STOPPED - LaBelle Iron Works, Thirtieth & Wood Streets, Wheeling, Ohio County, WV

  12. 47. INTERIOR VIEW, DETAIL OF CONVEYOR BELT SYSTEM SYSTEM WITH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    47. INTERIOR VIEW, DETAIL OF CONVEYOR BELT SYSTEM SYSTEM WITH BACK BELT DROPPING HARDENED NAILS ON THE FRONT BELT TO BE TEMPERED; MOTION STOPPED - LaBelle Iron Works, Thirtieth & Wood Streets, Wheeling, Ohio County, WV

  13. 42. INTERIOR VIEW OF THE NAIL HARDENER USED TO HARDEN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    42. INTERIOR VIEW OF THE NAIL HARDENER USED TO HARDEN AND TEMPER THE NAILS; WEST TUBES IN FOREGRPUND AND DRAWBACK TUBE IN THE CENTER - LaBelle Iron Works, Thirtieth & Wood Streets, Wheeling, Ohio County, WV

  14. The Public Use Limitation in Eminent Domain: "Handley v. Cook."

    ERIC Educational Resources Information Center

    Grill, Donna P.

    1979-01-01

    It is time for the courts to rigorously scrutinize allegations of public use in order to protect the property rights of private individuals. Available from West Virginia Law Review, W.V.U. Law Center, Morgantown, WV 26506. (Author)

  15. 3. Photocopied August 1975 from The Evolution of Modern Band ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Photocopied August 1975 from The Evolution of Modern Band Saw Mills for Sawing Logs, Dewitt Clinton Prescott, 1910. VIEW OF BAND SAW. *NOTE* 4'x 5' NEGATIVE - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  16. 4. Photocopied August 1975 from The Evolution of Modern Band ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Photocopied August 1975 from The Evolution of Modern Band Saw Mills for Sawing Logs, Dewitt Clinton Prescott, 1910. THREE-BLOCK LOG CARRIAGE. *NOTE* 4'x 5' NEGATIVE - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  17. 1. NORTHWEST FRONT, SOUTHWEST SIDE (SPRING HOUSE IN FOREGROUND; BATH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. NORTHWEST FRONT, SOUTHWEST SIDE (SPRING HOUSE IN FOREGROUND; BATH HOUSE AT REAR) (4 x 5 negative; 5 x 7 print) - Salt Sulphur Springs, Spring House, U.S. Route 219, Salt Sulphur Springs, Monroe County, WV

  18. 2. SHOWING (LEFT TO RIGHT) CHAPEL, STORE BUILDING, SPRING HOUSE, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. SHOWING (LEFT TO RIGHT) CHAPEL, STORE BUILDING, SPRING HOUSE, AND BATH HOUSE, SOUTHEAST FACADES (4 x 5 negative; 5 x 7 print) - Salt Sulpher Springs, U.S. Route 219, Salt Sulphur Springs, Monroe County, WV

  19. 9. INTERIOR VIEW OF EYE PRESS AREA; TO LEFT IS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. INTERIOR VIEW OF EYE PRESS AREA; TO LEFT IS A TWO-BAY SLOTTED TYPE FURNACE, TO CENTER IS EYE PRESS, TO RIGHT IS CURCULAR SAW - Warwood Tool Company, Foot of Nineteenth Street, Wheeling, Ohio County, WV

  20. 31 CFR 353.1 - Official agencies.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... half), OH, PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern...

  1. 31 CFR 352.13 - Fiscal agents.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern half), VA,...

  2. 31 CFR 315.1 - Official agencies.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... half), OH, PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern...

  3. 31 CFR 315.1 - Official agencies.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... half), OH, PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern...

  4. 31 CFR 353.1 - Official agencies.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... half), OH, PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern...

  5. 31 CFR 353.1 - Official agencies.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... half), OH, PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern...

  6. 31 CFR 315.1 - Official agencies.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... (eastern half), NJ (southern half), OH, PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern...

  7. 31 CFR 330.9 - Fiscal agents.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... half), OH, PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern...

  8. 31 CFR 352.13 - Fiscal agents.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern half), VA,...

  9. 31 CFR 352.13 - Fiscal agents.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern half), VA,...

  10. 31 CFR 352.13 - Fiscal agents.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... half), OH, PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern...

  11. 31 CFR 353.1 - Official agencies.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... half), OH, PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern...

  12. 31 CFR 315.1 - Official agencies.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... half), OH, PA, WV (northern panhandle). Federal Reserve Bank of Richmond, P.O. Box 27622, Richmond, VA 23261 Richmond, Atlanta AL, DC, FL, LA (southern half), MD, MS (southern half), NC, SC, TN (eastern...

  13. 45. William E. Barrett, Photographer, August 1975. EARLY STEAM GENERATING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    45. William E. Barrett, Photographer, August 1975. EARLY STEAM GENERATING UNIT USED TO PRODUCE ELECTRICITY FOR MANUFACTURING OPERATIONS AND FOR THE TOWN OF RAINELLE. STEAM ENGINE IS A HAMILTON CORLISS. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  14. 1. LOOKING SOUTHWEST TOWARD NORTH AND WEST SIDES OF OFFICE; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. LOOKING SOUTHWEST TOWARD NORTH AND WEST SIDES OF OFFICE; LABORATORY AND ELECTRICITY SUPPLY HOUSE TO THE RIGHT. - Standard Lime & Stone Quarry, Office, County Route 27, Millville, Jefferson County, WV

  15. 75 FR 13486 - Application(s) for Duty-Free Entry of Scientific Instruments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-22

    ..., WV 26506. Instrument: Electron Microscope. Manufacturer: JEOL, Japan. Intended Use: The instrument... domestic manufacturers of this type of electron microscope. Application accepted by Commissioner of Customs... 13617. Instrument: Electron Microscope. Manufacturer: FEI, Czech Republic. Intended Use: This...

  16. 5. DETAIL OF THE INSIDE ROBERTS AND SCHAEFER LOADING BOOM, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. DETAIL OF THE INSIDE ROBERTS AND SCHAEFER LOADING BOOM, WITH COUNTERWEIGHT (RIGHT), NOTE METAL CONVEYOR BELT (FOREGROUND) - Nuttallburg Mine Complex, Tipple, North side of New River, 2.7 miles upstream from Fayette Landing, Lookout, Fayette County, WV

  17. 27 CFR 9.111 - Kanawha River Valley.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...., dated 1958; (12) Sissonville, W. Va., dated 1958; (13) Romance, W. Va.,—Ky., dated 1957; (14) Kentuck, W... Johns Branch and Sugar Creek in the town of Romance, in Jackson County, WV. (Romance quadrangle) (8)...

  18. 27 CFR 9.111 - Kanawha River Valley.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...., dated 1958; (12) Sissonville, W. Va., dated 1958; (13) Romance, W. Va.,—Ky., dated 1957; (14) Kentuck, W... Johns Branch and Sugar Creek in the town of Romance, in Jackson County, WV. (Romance quadrangle) (8)...

  19. 27 CFR 9.111 - Kanawha River Valley.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...., dated 1958; (12) Sissonville, W. Va., dated 1958; (13) Romance, W. Va.,—Ky., dated 1957; (14) Kentuck, W... Johns Branch and Sugar Creek in the town of Romance, in Jackson County, WV. (Romance quadrangle) (8)...

  20. 27 CFR 9.111 - Kanawha River Valley.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...., dated 1958; (12) Sissonville, W. Va., dated 1958; (13) Romance, W. Va.,—Ky., dated 1957; (14) Kentuck, W... Johns Branch and Sugar Creek in the town of Romance, in Jackson County, WV. (Romance quadrangle) (8)...

  1. 27 CFR 9.111 - Kanawha River Valley.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...., dated 1958; (12) Sissonville, W. Va., dated 1958; (13) Romance, W. Va.,—Ky., dated 1957; (14) Kentuck, W... Johns Branch and Sugar Creek in the town of Romance, in Jackson County, WV. (Romance quadrangle) (8)...

  2. 21. Credit WS. Ground floor, Midget Marvel milling machine, a ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. Credit WS. Ground floor, Midget Marvel milling machine, a 25 barrel day machine by the Anglo American Mill Co. (Owensboro, KY), without covers, exposing rollers and sifters. - Bunker Hill Mill, County Route 26, Bunker Hill, Berkeley County, WV

  3. Anchor Casting and Portal Strut at West Bank Abutment, Endpost ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Anchor Casting and Portal Strut at West Bank Abutment, Endpost Base and Granite Plinth - Baltimore & Ohio Railroad, Bollman Bridge, Spanning Potomac River at Harpers Ferry, Harpers Ferry, Jefferson County, WV

  4. 14. INTERIOR OF POWER PLANT LOOKING SOUTHEAST AT ELECTRICAL PANEL. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. INTERIOR OF POWER PLANT LOOKING SOUTHEAST AT ELECTRICAL PANEL. - Potomac Power Plant, On West Virginia Shore of Potomac River, about 1 mile upriver from confluence with Shenandoah River, Harpers Ferry, Jefferson County, WV

  5. 63. Photocopy of photograph, no date. MAP OF POTOMAC EDISON ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    63. Photocopy of photograph, no date. MAP OF POTOMAC EDISON COMPANY SYSTEM. (Courtesy of the Potomac Edison Company Library (Hagerstown, MD), Historical Data Files - Dam No. 5 Hydroelectric Plant, On Potomac River, Hedgesville, Berkeley County, WV

  6. DETAIL OF STANDARD INTERLOCKING MACHINE OPERATING LEVERS. LOCKING MECHANISM IS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL OF STANDARD INTERLOCKING MACHINE OPERATING LEVERS. LOCKING MECHANISM IS BELOW FLOOR. BOXES BEHIND SOME LEVERS HOUSE ELECTRICAL CONTACTS FOR SIGNALS. - Baltimore & Ohio Railroad, Z Tower, State Route 46, Keyser, Mineral County, WV

  7. Preparation and characterization of malonic acid cross-linked chitosan and collagen 3D scaffolds: an approach on non-covalent interactions.

    PubMed

    Mitra, Tapas; Sailakshmi, G; Gnanamani, A; Mandal, A B

    2012-05-01

    The present study emphasizes the influence of non-covalent interactions on the mechanical and thermal properties of the scaffolds of chitosan/collagen origin. Malonic acid (MA), a bifuncitonal diacid was chosen to offer non-covalent cross-linking. Three dimensional scaffolds was prepared using chitosan at 1.0% (w/v) and MA at 0.2% (w/v), similarly collagen 0.5% (w/v) and MA 0.2% (w/v) and characterized. Results on FT-IR, TGA, DSC, SEM and mechanical properties (tensile strength, stiffness, Young's modulus, etc.) assessment demonstrated the existence of non-covalent interaction between MA and chitosan/collagen, which offered flexibility and high strength to the scaffolds suitable for tissue engineering research. Studies using NIH 3T3 fibroblast cells suggested biocompatibility nature of the scaffolds. Docking simulation study further supports the intermolecular hydrogen bonding interactions between MA and chitosan/collagen.

  8. Detail view of wrenches used in maintenance of engines and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of wrenches used in maintenance of engines and compressors. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  9. Detail view of lubricating oil pumps used in maintenance of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of lubricating oil pumps used in maintenance of the engines and compressors. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  10. 7. VIEW WEST, FERNOW EXPERIMENTAL FOREST WELL HOUSE, FERNOW EXPERIMENTAL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. VIEW WEST, FERNOW EXPERIMENTAL FOREST WELL HOUSE, FERNOW EXPERIMENTAL FOREST BUNKHOUSE, FERNOW EXPERIMENTAL FOREST GARAGE, AND FERNOW EXPERIMENTAL FOREST RESIDENCE. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  11. 77 FR 8276 - Agency Information Collection Activities: Proposed Collection; Comments Requested: Certificate of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-14

    ... instructions or additional information, please contact Tracey Robertson, Acting Chief, Federal Firearms Licensing Center, 244 Needy Road, Martinsburg, WV 25405 or via email at tracey.robertson@atf.gov...

  12. 35. Credit JTL. Third floor, side view of Monitor Dustless ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    35. Credit JTL. Third floor, side view of Monitor Dustless Receiving Separator, by Huntley, Cranson and Hammond, (Silver Creek NY) capacity of 100 barrels/day. - Bunker Hill Mill, County Route 26, Bunker Hill, Berkeley County, WV

  13. Detail exterior view looking north showing piping system adjacent to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail exterior view looking north showing piping system adjacent to engine house. Gas cooling system is on far right. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  14. Detail exterior view looking southwest of gas cooling system. Engine ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail exterior view looking southwest of gas cooling system. Engine house is shown in right background. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  15. Detail view of oil container designed as part of lubricating ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of oil container designed as part of lubricating system for unit 43. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  16. Detail view of valve mechanisms and goverenor on high pressure ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of valve mechanisms and goverenor on high pressure stage engine of unit 43. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  17. Detail exterior view looking northwest showing field gas cleaner in ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail exterior view looking northwest showing field gas cleaner in the center. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  18. Exterior view looking northwest with construction facilities of "new" pumping ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Exterior view looking northwest with construction facilities of "new" pumping station in foreground. Doug Hine is man in picture. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  19. DETAIL VIEW OF STEAM CHEST FOR LOW PRESSURE STAGE ENGINE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL VIEW OF STEAM CHEST FOR LOW PRESSURE STAGE ENGINE OF UNIT #3. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  20. General interior view of pumphouse looking southwest. Compressor unit 40 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General interior view of pumphouse looking southwest. Compressor unit 40 is in foreground. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  1. Detail view of steam chest for low pressure stage of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of steam chest for low pressure stage of unit 40. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  2. Detail view of gauges that record pressure of gas leaving ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of gauges that record pressure of gas leaving the engine house. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  3. Detail view of gauges that record pressure of gas coming ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of gauges that record pressure of gas coming into the engine house. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  4. General exterior view looking north. Boiler house is center/left. Engine ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General exterior view looking north. Boiler house is center/left. Engine house is center right. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  5. Detail view of unit 43 with high pressure stage compressor ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of unit 43 with high pressure stage compressor in left foreground. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  6. Detail view of condensor located below the floor level of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of condensor located below the floor level of the pumphouse. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  7. DETAIL VIEW OF OIL CONTAINER DESIGNED AS PART OF LUBRICATING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL VIEW OF OIL CONTAINER DESIGNED AS PART OF LUBRICATING SYSTEM FOR UNIT #3. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  8. Exterior view looking north/northwest showing water intake from Little Kanawha ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Exterior view looking north/northwest showing water intake from Little Kanawha River on right. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  9. Detail view of feedwater pump (below) and preheater tanks (above) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of feedwater pump (below) and preheater tanks (above) in boiler house. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  10. General view looking north showing boilers with units 47 and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General view looking north showing boilers with units 47 and 48 in right foreground. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  11. Exterior view of boiler house looking southwest with steam exhaust ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Exterior view of boiler house looking southwest with steam exhaust vents in foreground. Engine house is on left. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  12. Detail view of steam chest and valve mechanisms for high ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of steam chest and valve mechanisms for high pressure stage of unit 40. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  13. DETAIL VIEW OF UNIT #3 WITH HIGH PRESSURE STAGE COMPRESSOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL VIEW OF UNIT #3 WITH HIGH PRESSURE STAGE COMPRESSOR IN LEFT FOREGROUND. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  14. View of steam powered air compressor in boiler house. Gas ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of steam powered air compressor in boiler house. Gas engine powered electric generators are visible in far left background. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  15. Interior view of boiler house looking south. Boiler units are ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of boiler house looking south. Boiler units are on left. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  16. Detail view of steam chest for low pressure stage engine ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of steam chest for low pressure stage engine of unit 43. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  17. 35. INTERIOR VIEW, WHEELBRATORFRYE SHOT PEENER FOR REMOVAL OF RUST ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    35. INTERIOR VIEW, WHEELBRATOR-FRYE SHOT PEENER FOR REMOVAL OF RUST AND SCALE; NOTE TOOLS ARE TUMBLED WITH BLASTED WITH LEAD SHOT TO CLEAN SURFACES - Warwood Tool Company, Foot of Nineteenth Street, Wheeling, Ohio County, WV

  18. 9. TRANSMISSION GEARING FROM SPUR WHEEL (right) USED TO INCREASE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. TRANSMISSION GEARING FROM SPUR WHEEL (right) USED TO INCREASE REVOLUTIONS PER MINUTE William E. Barrett, photographer, 1973 - Thomas Shepherd's Grist Mill, High Street Vicinity, Shepherdstown, Jefferson County, WV

  19. 19. LOOKING NORTH ALONG ROAD BISECTING SITE; PEBBLE LIME SILO ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. LOOKING NORTH ALONG ROAD BISECTING SITE; PEBBLE LIME SILO ON THE RIGHT, MAIN SUPPLY BUILDING AND MACHINE SHOP ON THE LEFT. - Standard Lime & Stone Quarry, County Route 27, Millville, Jefferson County, WV

  20. East yard, north elevation of car department tool house (converted ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    East yard, north elevation of car department tool house (converted from express car). - Chesapeake & Ohio Railroad, Thurmond Yards, East side New River, mouths of Arbuckle & Dunlop Circles, Thurmond, Fayette County, WV

  1. Spruce No. 1 Surface Mine Recommended Determination

    EPA Pesticide Factsheets

    This document explains the basis for the EPA Region III recommendation to withdraw the specification of Pigeonroost Branch, Oldhouse Branch and their tributaries within Logan County, WV as a disposal site for dredged or fill material.

  2. 3. Photocopied 1974 from The Baltimore and Ohio Railroad and ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Photocopied 1974 from The Baltimore and Ohio Railroad and Its Branches, from the Lakes to the Sea, Baltimore, 1872. EARLY VIEW AT TRACK LEVEL. - Baltimore & Ohio Railroad, Tray Run Viaduct, Spanning Tray Run, Rowlesburg, Preston County, WV

  3. 1. LOOKING WEST TOWARD OIL/CLEANING SUPPLY BUILDING (SIDING AND ROOF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. LOOKING WEST TOWARD OIL/CLEANING SUPPLY BUILDING (SIDING AND ROOF VISIBLE TO THE LEFT); GAS PUMP SHED IN CENTER (IN SHADOWS). - Standard Lime & Stone Quarry, Oil-Cleaning Supply Building, County Route 27, Millville, Jefferson County, WV

  4. 32. William E. Barrett, Photographer, 1973. DEADEND WELL WITH WOODEN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    32. William E. Barrett, Photographer, 1973. DEAD-END WELL WITH WOODEN OIL TANK, PIPE ROCKER ARM. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  5. 41. William E. Barrett, Photographer, 1973. MR. WEST'S HOUSE, MR. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    41. William E. Barrett, Photographer, 1973. MR. WEST'S HOUSE, MR. WEST ON PORCH AND THE ARBOR THAT IS OVER 100 YEARS OLD. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  6. 27. Photocopied 1973 from The Rig & Reel Magazine, (March, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. Photocopied 1973 from The Rig & Reel Magazine, (March, 1923) p. 5. EARLIER ENDLESS-WIRE TRANSMISSION NOW IN RUINS. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  7. 31. William E. Barrett, Photographer, 1973. WELL WITH POLE DERRICK ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    31. William E. Barrett, Photographer, 1973. WELL WITH POLE DERRICK AND COMPLETE SET OF WHEELS, ROADWAY ACCESS. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  8. 40. William E. Barrett, Photographer, 1973. SHOP, YPOLE, MR. WEST'S ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    40. William E. Barrett, Photographer, 1973. SHOP, Y-POLE, MR. WEST'S HOUSE AND POLE SWELL - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  9. 34. William E. Barrett, Photographer, 1973. ENGINE HOUSE, WIRES FROM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    34. William E. Barrett, Photographer, 1973. ENGINE HOUSE, WIRES FROM IT, AND COMPLETE WELL DERAIL, WHEELS. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  10. 28. Photocopied 1973 from The Rig & Reel Magazine, (March, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    28. Photocopied 1973 from The Rig & Reel Magazine, (March, 1923) p. 2. INTERIOR OF CENTRAL POWERHOUSE AT VOLCANO - NOW DISMANTLED. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  11. 26. Photocopied 1973 from The Rig & Reel Magazine, (March, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    26. Photocopied 1973 from The Rig & Reel Magazine, (March, 1923) p. 1. TYPICAL WELL HOOK-UP. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  12. 25. Photocopied 1973 from The Rig & Reel Magazine, (March, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    25. Photocopied 1973 from The Rig & Reel Magazine, (March, 1923) p. 1. TYPICAL ENDLESS-WIRE RIG IN VOLCANO. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  13. 46. William E. Barrett, Photographer, 1977. SAME SHOWING MORE ROOF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    46. William E. Barrett, Photographer, 1977. SAME SHOWING MORE ROOF DAMAGE AND NOT QUITE AS WIDE AN ANGLE VIEW. - West Oil Company Endless Wire Pumping Station, U.S. Route 50 (Volcano vicinity), Petroleum, Ritchie County, WV

  14. 3. WEST ELEVATION, SHOWING CHECK WEIGHMAN'S ROOM (LEFT CENTER), CONVEYOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. WEST ELEVATION, SHOWING CHECK WEIGHMAN'S ROOM (LEFT CENTER), CONVEYOR MOTOR ROOM (RIGHT CENTER) AND CONVEYOR (RIGHT), LOOKING SOUTHEAST - Nuttallburg Mine Complex, Headhouse, North side of New River, 2.7 miles upstream from Fayette Landing, Lookout, Fayette County, WV

  15. 8. MACHINE SHOP, INTERIOR, LOOKING AT ROOF TRUSS SYSTEM. NOTE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. MACHINE SHOP, INTERIOR, LOOKING AT ROOF TRUSS SYSTEM. NOTE DECORATIVE WOOD COLUMN AT LOWER LEFT. - Baltimore & Ohio Railroad, Keyser Machine Shop, State Route 46 Northwest of Spring Street, Keyser, Mineral County, WV

  16. 8. Double crib barn, south corner, log section, loft area, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Double crib barn, south corner, log section, loft area, detail of log construction - Wilkins Farm, Barn, South side of Dove Hollow Road, 6000 feet east of State Route 259, Lost City, Hardy County, WV

  17. Libraries in West Virginia: MedlinePlus

    MedlinePlus

    ... this page: https://medlineplus.gov/libraries/westvirginia.html Libraries in West Virginia To use the sharing features ... enable JavaScript. Bridgeport United Hospital Center Health Sciences Library 327 Medical Park Dr Bridgeport, WV 26330 681- ...

  18. 77 FR 29447 - Qualification of Drivers; Exemption Applications; Vision

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-17

    .... Allen (OR) James C. Askin (FL) Ernie E. Black (NC) Gary O. Brady (WV) Stephen H. Goldcamp (OH) Hazel L. Hopkins, Jr. (MD) Wai F. King (IL) Dennis E. Krone (IL) Richard J. McKenzie, Jr. (MD) Christopher...

  19. 78 FR 44874 - IFR Altitudes; Miscellaneous Amendments

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-25

    ... Point From To MEA MAA Sec. 95.3000 Low Altitude RNAV Routes Sec. 95.3291 RNAV Route T291 Is Added To...--GNSS MEA *DME/DME/IRU MEA KOATA, OR WP KIMBERLY, OR VORTAC *29000 45000 *18000--GNSS MEA *DME/DME/IRU MEA Sec. 95.4068 RNAV Route Q68 Is Added To Read CHARLESTON, WV VORTAC TOMCA, WV WP *18000...

  20. Topical Hazard Evaluation Program Procedural Guide.

    DTIC Science & Technology

    1982-01-01

    conditions and are percent (w/v) Oil of tion reaction under test not expected to cause a Bergamot solution conditions. photochemical irritation...photochemical skin irritant ( Bergamot oil). d. All compounds-are handled with caution. Current test procedures cannot eliminate the possibility of individual...percent ethyl alcohol. One additional compound applied along with the test compounds is a 10 percent solution (w/v) of Bergamot oil" in 95 percent ethyl

  1. Corynebacterium marinum sp. nov. isolated from coastal sediment in Qingdao, China

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A taxonomic study was performed on strain D7015T, which was isolated from coastal sediment close to a coal-fired power station in Qingdao, China. D7015T comprised Gram-positive, non-motile diphtheroid rods, which grew in 0-10 % (w/v) NaCl and 4-37°C, with optimum growth at 1% (w/v) NaCl and 30-32°C...

  2. Relativistic Jets in the Radio Reference Frame Image Database. 1. Apparent Speeds From the First 5 Years of Data

    DTIC Science & Technology

    2007-02-11

    astronomical community have very good coverage in the RRFID. Because of this historical evolution of the source list, the exact nature of the biases...geodetic antennas (Gilcreek: Fairbanks, AK, USA; NRAO 20 cm: Green Bank, WV, USA; Kokee: Kokee Park, HI, USA; Medicina : Medicina , Italy; Ny Alesund...Fairbanks, AK, USA; Gn: NRAO 20 cm, Green Bank,WV, USA; Kk: Kokee, Kokee Park, HI, USA; Mc: Medicina , Medicina , Italy; Ny: Ny Alesund 20 cm, Ny Alesund

  3. Decrease of growth and aflatoxin production in Aspergillus parasiticus caused by spices.

    PubMed

    Olojede, F; Engelhardt, G; Wallnofer, P R; Adegoke, G O

    1993-09-01

    Non-commercial spices and herbs Tetrapleura tetrapetra, Triumfetta cordifolia, Garcina kola, Monodora myristica and Xylopia aethiopica at 0.08 to 0.32% (w/v) decreased the mycelial weight of Aspergillus parasiticus NRRL 2999 in yeast extract/sucrose broth by up to 68%. Aflatoxin production, monitored with ELISA, was most effectively decreased, from 97 to 23 μg/ml, when the extract of G. kola was added at 0.32% (w/v).

  4. 24. Photo copy of photograph, (original in Forest Service Office, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    24. Photo copy of photograph, (original in Forest Service Office, Elkins, WV, photo #397508, 'Women weeding in the one-year old red spruce seedlings at the Parsons Nursery at Parsons, W. Va. Women are used for weeding here because of the dexterity of their hands. They are paid 40 cents an hour'), B. W. Muir, 1940. VIEW NORTHEAST, PACKING BUILDING IN BACKGROUND, WOMEN WEEDING. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  5. Flavobacterium faecale sp. nov., an agarase-producing species isolated from stools of Antarctic penguins.

    PubMed

    Kim, Jin Ho; Choi, Bo Hyun; Jo, Minho; Kim, Sun Chang; Lee, Pyung Cheon

    2014-08-01

    Taxonomic studies were performed on an agarase-producing strain, designated WV33(T), isolated from faeces of Antarctic penguins. Cells of strain WV33(T) were Gram-staining-negative, strictly aerobic, orange and rod-shaped. Strain WV33(T) displayed agarase activity and was able to utilize galactose as a sole carbon source. 16S rRNA gene sequence analysis revealed that strain WV33(T) was closely related to Flavobacterium algicola TC2(T) (98.0% similarity), F. frigidarium ATCC 700810(T) (96.9%) and F. frigoris LMG 21922(T) (96.1%). The predominant cellular fatty acids were iso-C(15 : 1) G, iso-C(15 : 0), C(15 : 0), C(16 : 0) and summed feature 3 (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c). Menaquinone 6 (MK-6) was the sole quinone identified, and the major pigment was zeaxanthin. The major polar lipid was phosphatidylethanolamine. DNA-DNA relatedness of strain WV33(T) with respect to its closest phylogenetic neighbours was 25% for F. algicola NBRC 102673(T), 23% for F. frigidarium DSM 17623(T) and 21% for F. frigoris DSM 15719(T). The DNA G+C content of strain WV33(T) was 37±0.6 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain WV33(T) is concluded to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium faecale sp. nov. is proposed. The type strain is WV33(T) ( = KCTC 32457(T) = CECT 8384(T)).

  6. WorldView-2 and the evolution of the DigitalGlobe remote sensing satellite constellation: introductory paper for the special session on WorldView-2

    NASA Astrophysics Data System (ADS)

    Anderson, Neal T.; Marchisio, Giovanni B.

    2012-06-01

    Over the last decade DigitalGlobe (DG) has built and launched a series of remote sensing satellites with steadily increasing capabilities: QuickBird, WorldView-1 (WV-1), and WorldView-2 (WV-2). Today, this constellation acquires over 2.5 million km2 of imagery on a daily basis. This paper presents the configuration and performance capabilities of each of these satellites, with emphasis on the unique spatial and spectral capabilities of WV-2. WV-2 employs high-precision star tracker and inertial measurement units to achieve a geolocation accuracy of 5 m Circular Error, 90% confidence (CE90). The native resolution of WV-2 is 0.5 m GSD in the panchromatic band and 2 m GSD in 8 multispectral bands. Four of the multispectral bands match those of the Landsat series of satellites; four new bands enable novel and expanded applications. We are rapidly establishing and refreshing a global database of very high resolution (VHR) 8-band multispectral imagery. Control moment gyroscopes (CMGs) on both WV-1 and WV-2 improve collection capacity and provide the agility to capture multi-angle sequences in rapid succession. These capabilities result in a rich combination of image features that can be exploited to develop enhanced monitoring solutions. Algorithms for interpretation and analysis can leverage: 1) broader and more continuous spectral coverage at 2 m resolution; 2) textural and morphological information from the 0.5 m panchromatic band; 3) ancillary information from stereo and multi-angle collects, including high precision digital elevation models; 4) frequent revisits and time-series collects; and 5) the global reference image archives. We introduce the topic of creative fusion of image attributes, as this provides a unifying theme for many of the papers in this WV-2 Special Session.

  7. The Evolution of Teamwork Skills: An Empirical Assessment with Implications for Training

    DTIC Science & Technology

    1987-11-15

    session. 29 Technical Report 87-016 DATA COLLECTION FORMS For ms Critica TeamBevios r. A critical incident approach (Flanagan, 1954; Glickman & Vallance...2 1 BRIDGE TALKER 5 4 3 2 1 PLEASE CIRCLE SESSION: PRE-MIDTERM MIDTERM POST-MIDTERM 104 Hill Rvvu~ww w wu w-rvi~wu wV ww wu ww " wv wVwV " w ’ w~wu

  8. An Algorithm for the Computation of Generalized Likelihood or Self-Critical Estimators for Binary Data.

    DTIC Science & Technology

    1986-01-01

    Polyteci IUtba DTIC INSIECTED p; 6 5% Js. * - r . S- .rm r..,w-rg J wrr T Ir: V, W v WVr W w r f WV rWWwWV my~f w SM w%%ir I Doo~msntatlan for Self...distributiJon, logistic di iin a-,ssian distribution, generalized likelihood, model- critica . analys is, regression models, parametric proportional hazard

  9. Optimization of culture medium compositions for gellan gum production by a halobacterium Sphingomonas paucimobilis.

    PubMed

    Zhang, Jun; Dong, Ya-chen; Fan, Lin-lin; Jiao, Zhi-hua; Chen, Qi-he

    2015-01-22

    The effect of culture medium compositions on gellan gum production produced by fermentation with a halobacterium Sphingomonas paucimobilis QHZJUJW CGMCC2428 was studied. In this work, a fractional factorial design was applied to investigate the main factors that affected gellan gum production by S. paucimobilis QHZJUJW CGMCC2428. Sucrose was the best carbon source for gellan gum and peptone displayed better inducing effect. Central composite design and response surface methodology were adopted to derive a statistical model for optimizing submerged culture medium composition. These experimental results showed that the optimum culture medium for producing gellan gum was composed of 40.00 (w/v) sucrose, 3.00% peptone (w/v), MgSO4 (w/v), 9.20% KH2PO4 (w/v), 7.50% Na2HPO4 (w/v), 4.30% K2SO4 (w/v), pH 6.8-7.0. The maximal gellan gum was 19.89±0.68 g/L, which was agreed closely with the predicated value (20.12 g/L). After incubated for 72 h under the optimized culture medium in 5-L bioreactor, the gellan gum fermentation reached about 19.90±0.68 g/L, which was higher than that in the initial cultivation medium.

  10. Syringe test (modified larval immersion test): a new bioassay for testing acaricidal activity of plant extracts against Rhipicephalus microplus.

    PubMed

    Sindhu, Zia-ud-Din; Jonsson, Nicholas N; Iqbal, Zafar

    2012-09-10

    We report a new bioassay "syringe test" (modified larval immersion test) for in vitro evaluation of acaricidal activity of crude plant extracts. Prepared syringes, containing eggs of tick, were incubated until 14 d after hatching of eggs, when the bioassay was performed on the larvae. Lethal concentrations for 50% of larvae (LC(50)), LC(90) and LC(99) values were calculated for each tested product. 95% confidence intervals for LC(50) were very narrow, indicating a high degree of repeatability for the new bioassay on larvae of R. microplus. Bioassays were applied to six crude aqueous-methanol extracts from five plants (Acacia nilotica, Buxus papillosa, Fumaria parviflora, Juniperus excelsa, and Operculina turpethum), of which three showed discernible effects. Twenty-four hours post exposure, LC(99) values were 11.9% (w/v) for F. parviflora, 20.8% (w/v) and 29.2% (w/v) for B. papillosa and A. nilotica, respectively. After six days of exposure these values were; 9.1% (w/v), 9.2% (w/v) and 15.5 (w/v) for F. parviflora, A. nilotica and B. papillosa, respectively.

  11. Assessment of Pansharpening Methods Applied to WorldView-2 Imagery Fusion

    PubMed Central

    Li, Hui; Jing, Linhai; Tang, Yunwei

    2017-01-01

    Since WorldView-2 (WV-2) images are widely used in various fields, there is a high demand for the use of high-quality pansharpened WV-2 images for different application purposes. With respect to the novelty of the WV-2 multispectral (MS) and panchromatic (PAN) bands, the performances of eight state-of-art pan-sharpening methods for WV-2 imagery including six datasets from three WV-2 scenes were assessed in this study using both quality indices and information indices, along with visual inspection. The normalized difference vegetation index, normalized difference water index, and morphological building index, which are widely used in applications related to land cover classification, the extraction of vegetation areas, buildings, and water bodies, were employed in this work to evaluate the performance of different pansharpening methods in terms of information presentation ability. The experimental results show that the Haze- and Ratio-based, adaptive Gram-Schmidt, Generalized Laplacian pyramids (GLP) methods using enhanced spectral distortion minimal model and enhanced context-based decision model methods are good choices for producing fused WV-2 images used for image interpretation and the extraction of urban buildings. The two GLP-based methods are better choices than the other methods, if the fused images will be used for applications related to vegetation and water-bodies. PMID:28067770

  12. Engraftment of bone marrow transplants in W anemic mice measured by electronic determination of the red blood cell size profile.

    PubMed

    Wiktor-Jedrzejczak, W; Sharkis, S J; Ahmed, A; Sensenbrenner, L L; Sell, K W

    1979-09-01

    Defective stem cells of WBB6F1-W/Wv mice produce macrocytic red blood cells (RBCs); stem cells of WBB6F1-+/+ mice produce normocytic RBCs. Utilization of the Coulter counter channelyzer permitted good dissociation between the size distribution of populations of +/+ and W/Wv RBCs. Peaks (mean cell volumes) for +/+ and W/Wv RBCs have been determined to be between the 30th and 40th channel and 50th and 60th channel, respectively. Variability of profiles for individual mice of both genotypes did not exceed the variability of separate determinations of the same cell suspension from a single mouse. Admixture (approximately 15%) of either type of erythrocytes could be quantitatively detected by this method. One week after transplant of 10(7) +/+ marrow cells into W/Wv recipients, 25% of donor type erythrocytes were detected. Eighteen days post-graft, concentration of +/- normocytes exceeded the concentration of macrocytes in the W/Wv recipients' circulation. Approximately 45 days post-transplant, the proportion of macrocytes decreased below the 10% detectable level. Calculation of the daily RBC production rate during repopulation and estimation of the number of RBCs produced by a single hematopoietic colony were determined. The RBC size profile was found to be a convenient method for studying the effect of implantation of W/Wv marrow into lethally irradiated +/+ mice. This method proved suitable for repetitive determination of the size population in individual transplanted mice.

  13. Mast cells modulate acute ozone-induced inflammation of the murine lung

    SciTech Connect

    Kleeberger, S.R.; Seiden, J.E.; Levitt, R.C.; Zhang, L.Y. )

    1993-11-01

    We hypothesized that mast cells modulate lung inflammation that develops after acute ozone (O3) exposure. Two tests were done: (1) genetically mast-cell-deficient (WBB6F1-W/Wv, WCB6F1-SI/SId) and bone-marrow-transplanted W/Wv mice were exposed to O3 or filtered air, and the inflammatory responses were compared with those of mast-cell-sufficient congenic mice (WBB6F1-(+)/+, WCB6F1-(+)/+); (2) genetically O3-susceptible C57BL/6J mice were treated pharmacologically with putative mast-cell modulators or vehicle, and the O3-induced inflammatory responses were compared. Mice were exposed to 1.75 ppm O3 or air for 3 h, and lung inflammation was assessed by bronchoalveolar lavage (BAL) 6 and 24 h after exposure. Relative to O3-exposed W/Wv and SI/SId mice, the mean numbers of lavageable polymorphonuclear leukocytes (PMNs) and total BAL protein concentration (a marker of permeability) were significantly greater in the respective O3-exposed normal congenic +/+ mice (p < 0.05). Mast cells were reconstituted in W/Wv mice by transplantation of bone marrow cells from congenic +/+ mice, and O3-induced lung inflammation was assessed in the mast-cell-replete W/Wv mice. After O3 exposure, the changes in lavageable PMNs and total protein of mast-cell-replete W/Wv mice were not different from age-matched normal +/+ control mice, and they were significantly greater than those of sham-transplanted W/Wv mice (p < 0.05). Genetically susceptible C57BL/6J mice were pretreated with a mast-cell stabilizer (nedocromil sodium), secretagogue (compound 48/80), or vehicle, and the mice were exposed to O3.

  14. Intranasal immunization with a formalin-inactivated human influenza A virus whole-virion vaccine alone and intranasal immunization with a split-virion vaccine with mucosal adjuvants show similar levels of cross-protection.

    PubMed

    Okamoto, Shigefumi; Matsuoka, Sumiko; Takenaka, Nobuyuki; Haredy, Ahmad M; Tanimoto, Takeshi; Gomi, Yasuyuki; Ishikawa, Toyokazu; Akagi, Takami; Akashi, Mitsuru; Okuno, Yoshinobu; Mori, Yasuko; Yamanishi, Koichi

    2012-07-01

    The antigenicity of seasonal human influenza virus changes continuously; thus, a cross-protective influenza vaccine design needs to be established. Intranasal immunization with an influenza split-virion (SV) vaccine and a mucosal adjuvant induces cross-protection; however, no mucosal adjuvant has been assessed clinically. Formalin-inactivated intact human and avian viruses alone (without adjuvant) induce cross-protection against the highly pathogenic H5N1 avian influenza virus. However, it is unknown whether seasonal human influenza formalin-inactivated whole-virion (WV) vaccine alone induces cross-protection against strains within a subtype or in a different subtype of human influenza virus. Furthermore, there are few reports comparing the cross-protective efficacy of the WV vaccine and SV vaccine-mucosal adjuvant mixtures. Here, we found that the intranasal human influenza WV vaccine alone induced both the innate immune response and acquired immune response, resulting in cross-protection against drift variants within a subtype of human influenza virus. The cross-protective efficacy conferred by the WV vaccine in intranasally immunized mice was almost the same as that conferred by a mixture of SV vaccine and adjuvants. The level of cross-protective efficacy was correlated with the cross-reactive neutralizing antibody titer in the nasal wash and bronchoalveolar fluids. However, neither the SV vaccine with adjuvant nor the WV vaccine induced cross-reactive virus-specific cytotoxic T-lymphocyte activity. These results suggest that the intranasal human WV vaccine injection alone is effective against variants within a virus subtype, mainly through a humoral immune response, and that the cross-protection elicited by the WV vaccine and the SV vaccine plus mucosal adjuvants is similar.

  15. Exceptional hexose-fermenting ability of the xylitol-producing yeast Candida guilliermondii FTI 20037.

    PubMed

    Wen, Xin; Sidhu, Sukhdeep; Horemans, Spencer K C; Sooksawat, Najjapak; Harner, Nicole K; Bajwa, Paramjit K; Yuan, Zhirun; Lee, Hung

    2016-06-01

    The yeast Candida guilliermondii FTI 20037 is well-known for its ability to produce xylitol from xylose. Recently, this strain was found to produce greater than 5% (w/v) ethanol from glucose. This level of ethanol is typically not exceeded by wild-type strains of other native pentose-fermenting yeasts. This prompted the current study to examine the ability of C. guilliermondii FTI 20037 to utilize and ferment high concentrations of each of the hexoses commonly found in lignocellulosic hydrolysates. In defined media, FTI 20037 fermented 14.4%-25.9% (w/v) of glucose, mannose or galactose individually to ethanol in concentrations ranging from 6% to 9.3% (w/v). Fermentation was completed within 36 h (for glucose) to 100 h (for galactose). In 25.9% (w/v) glucose, FTI 20037 produced 9.3% (w/v) ethanol within 40 h. FTI 20037 produced xylitol exclusively when xylose was given as the sole carbon source. The strain utilized arabinose poorly. Under the same fermentation conditions, an industrial Saccharomyces cerevisiae strain produced slightly higher levels of ethanol [9.9% (w/v)] from 25.0% (w/v) glucose. Another pentose-fermenting yeast Pachysolen tannophilus also fermented high concentrations of glucose and mannose to produce relatively high peak ethanol concentrations; however, this yeast required considerably longer to completely consume these hexoses. The ability of FTI 20037 to produce high level of ethanol rapidly from glucose is remarkable. To our knowledge, this is the first known instance of a non-modified native xylose-fermenting yeast strain able to produce such high levels of ethanol from glucose as rapidly as S. cerevisiae in a defined medium.

  16. The longitudinal effects of resisted sprint training using weighted sleds vs. weighted vests.

    PubMed

    Clark, Kenneth P; Stearne, David J; Walts, Cory T; Miller, Anthony D

    2010-12-01

    The purpose of this study was to determine the longitudinal effects of weighted sled (WS) and weighted vest (WV) sprint training on maximum velocity sprint performance and kinematics. Twenty male collegiate lacrosse players were randomly assigned to a WS group (n = 7) towing 10% body mass, a WV group (n = 6) loaded with 18.5% body mass, or an unresisted (UR) active control group (n = 7). All subjects completed 13 training sessions over 7 weeks. Pre- and post-test measures of sprint time and average velocity across the distance interval of 18.3 to 54.9 m were used to assess sprint performance, whereas high-speed video (300 Hz) and motion-analysis software were used to analyze stride length, stride rate, ground contact time, and flight time. A 3 × 2 repeated measures analysis of variance was performed for each dependent variable and revealed no significant between-group differences for any of the sprint performance or kinematic stride cycle measures. Effect size statistics suggested small improvements in 18.3- to 54.9-m sprint time and average velocity for the UR group but only trivial improvements for the WS and WV groups. With regard to sprint performance, the results indicate that WS and WV training had no beneficial effect compared with UR training. In fact, for the loads used by WS and WV in this study, UR training may actually be superior for improving sprint performance in the 18.3- to 54.9-m interval.

  17. Quantifying Libya-4 Surface Reflectance Heterogeneity With WorldView-1, 2 and EO-1 Hyperion

    NASA Technical Reports Server (NTRS)

    Neigh, Christopher S. R.; McCorkel, Joel; Middleton, Elizabeth M.

    2015-01-01

    The land surface imaging (LSI) virtual constellation approach promotes the concept of increasing Earth observations from multiple but disparate satellites. We evaluated this through spectral and spatial domains, by comparing surface reflectance from 30-m Hyperion and 2-m resolution WorldView-2 (WV-2) data in the Libya-4 pseudoinvariant calibration site. We convolved and resampled Hyperion to WV-2 bands using both cubic convolution and nearest neighbor (NN) interpolation. Additionally, WV-2 and WV-1 same-date imagery were processed as a cross-track stereo pair to generate a digital terrain model to evaluate the effects from large (>70 m) linear dunes. Agreement was moderate to low on dune peaks between WV-2 and Hyperion (R2 <; 0.4) but higher in areas of lower elevation and slope (R2 > 0.6). Our results provide a satellite sensor intercomparison protocol for an LSI virtual constellation at high spatial resolution, which should start with geolocation of pixels, followed by NN interpolation to avoid tall dunes that enhance surface reflectance differences across this internationally utilized site.

  18. Formulation and optimization of ethosomes for transdermal delivery of ropinirole hydrochloride.

    PubMed

    Mishra, Ashish D; Patel, C N; Shah, Dinesh R

    2013-10-01

    The present study focuses on the formulation of ethosomal gel of ropinirole hydrochloride (ropinirole HCl), an anti-Parkinsonian drug, for delivery as a carrier for transdermal application. The ethosomes were prepared using different concentrations of phospholipids (2-5 % w/v), ethanol (20-50 % w/v), ropinirole HCl (5 % w/v) and water. They were optimized using 3(2) full factorial designs to study the effect of independent variables, concentrations of ethanol and lecithin on dependent variables, entrapment efficiency and in-vitro drug release at 24 hrs. The drug release profile exhibited Higuchi's and zero order kinetics. From the regression analysis, it was observed that independent variables had significant effect on response variables. Formulations were optimized using contour plot and response surface plot. The optimized formulation was found to be RS10 containing 30 % w/v ethanol and 4% w/v lecithin. The optimized formulation was evaluated for assay, particle characteristics, zeta potential, skin retention and stability. Ethosomal gel was prepared by incorporation of optimized ethosomal suspension into gel base. The ethosomal gel was characterized for physical appearance, pH, content uniformity, rheological behaviour, skin-retention, in-vitro and in-vivo drug release and stability. From the results it can fairly be concluded that ethosomes are capable of delivering ropinirole hydrochloride into systemic circulation by transdermal route. The amounts thus delivered are also equitable to those delivered orally and are delivered at a rate slow enough to achieve longer blood levels.

  19. Efficient ethanol production from separated parenchyma and vascular bundle of oil palm trunk.

    PubMed

    Prawitwong, Panida; Kosugi, Akihiko; Arai, Takamitsu; Deng, Lan; Lee, Kok Chang; Ibrahim, Darah; Murata, Yoshinori; Sulaiman, Othman; Hashim, Rokiah; Sudesh, Kumar; Ibrahim, Wan Asma Bt; Saito, Masayoshi; Mori, Yutaka

    2012-12-01

    For efficient utilization of both starchy and cellulosic materials, oil palm trunk was separated into parenchyma (PA) and vascular bundle (VB). High solid-state simultaneous saccharification and fermentation (HSS-SSF) using 30% (w/v) PA, containing 46.7% (w/w) starch, supplemented with amylases and Saccharomyces cerevisiae K3, produced 6.1% (w/v) ethanol. Subsequent alkali-pretreatment using sodium hydroxide was carried out with starch-free PA (sfPA) and VB. Enzymatic digestibility of 5% (w/v) pretreated sfPA and VB was 92% and 97%, respectively, using 18 FPU of commercial cellulase supplemented with 10 U of Novozyme-188 per gram of substrate. Likewise, HSS-SSF using 30% (w/v) alkali-pretreated sfPA and VB, with cellulases and yeast, resulted in high ethanol production (8.2% and 8.5% (w/v), respectively). These results show that HSS-SSF using separated PA and VB is a useful fermentation strategy, without loss of starchy and cellulosic materials, for oil palm trunk.

  20. Production, Purification, and Characterization of Polygalacturonase from Mucor circinelloides ITCC 6025

    PubMed Central

    Thakur, Akhilesh; Pahwa, Roma; Singh, Smarika; Gupta, Reena

    2010-01-01

    Mucor circinelloides produced an extracellular polygalacturonase enzyme, the production of which was enhanced when various production parameters were optimized. Maximum polygalacturonase (PGase) activity was obtained in 48 h at 30°C and pH 4.0 with pectin methyl ester (1% w/v) as carbon source and a combination of casein hydrolysate (0.1% w/v) and yeast extract (0.1% w/v) as nitrogen source. The enzyme was purified to homogeneity (13.3-fold) by Sephacryl S-100 gel-filtration chromatography. Its molecular weight was 66 kDa on SDS-PAGE. The enzyme was found to have Km and Vmax values of 2.2 mM and 4.81 IU/ml at 0.1% to 0.5% (w/v) concentration of the substrate. The addition of phenolic acids (0.05 mM), metal ions such as Mn+2, Co+2, Mg+2, Fe+3, Al+3, Hg+2, and Cu+2, and thiols had inhibitory effect on the enzyme. The enzyme showed maximum activity in the presence of polygalacturonic acid (0.1% w/v) at pH 5.5 and 42°C. PMID:21048861

  1. Formulation development of smart gel periodontal drug delivery system for local delivery of chemotherapeutic agents with application of experimental design.

    PubMed

    Dabhi, Mahesh R; Nagori, Stavan A; Gohel, Mukesh C; Parikh, Rajesh K; Sheth, Navin R

    2010-01-01

    Smart gel periodontal drug delivery systems (SGPDDS) containing gellan gum (0.1-0.8% w/v), lutrol F127 (14, 16, and 18% w/v), and ornidazole (1% w/v) were designed for the treatment of periodontal diseases. Each formulation was characterized in terms of in vitro gelling capacity, viscosity, rheology, content uniformity, in vitro drug release, and syringeability. In vitro gelation time and the nature of the gel formed in simulated saliva for prepared formulations showed polymeric concentration dependency. Drug release data from all formulations was fitted to different kinetic models and the Korsemeyer-Peppas model was the best fit model. Drug release was significantly decreased as the concentration of each polymer component was increased. Increasing the concentration of each polymeric component significantly increased viscosity, syringeability, and time for 50%, 70%, and 90% drug release. In conclusion, the formulations described offer a wide range of physical and drug release characteristics. The formulation containing 0.8% w/v of gellan gum and 16% w/v of lutrol F127 exhibited superior physical characteristics.

  2. From high tech to high touch: integrating community voices in mobile mammography outreach.

    PubMed

    Kennedy, Stephenie; Stephens, Sheila; Tephabock, Kevin; Brown, Pamela; Davis, Patty; Keresztury, James; Narsavage, Georgia

    2009-10-01

    Providing mobile screening mammography services across the state of West Virginia (WV) presents unique challenges. The ability of new machines to screen for breast cancer is modern medicine at its best. The use of the mobile unit, "Bonnie's Bus", holds promise for getting this high tech equipment to women in rural areas of West Virginia where it is most needed. Cancer detected in early stages is more treatable and women have a better chance of becoming survivors. The key to the program's success resides with the women who come for the screening, the extension network of community members who help set up and assist women to access the screening system, and community health care providers who care for the women if cancer is found. Linking "community voices" with the programs funded under the WV Komen grant screening programs provides a winning solution for West Virginia and the women served by Bonnie's Bus. Information obtained from five focus groups of 58 community leaders and women residing in rural WV was used to develop the mobile program. Building upon established relationships with the West Virginia Breast and Cervical Cancer Screening Program (WV BCCSP) coordinators and providers, outreach and information gleaned from WV communities, is being used to develop community partnerships based on mutual trust and respect to advance the common goal of decreasing breast cancer related illness and death in West Virginia women.

  3. Concentration selective hydration and phase states of hydroxyethyl cellulose (HEC) in aqueous solutions.

    PubMed

    Arfin, Najmul; Bohidar, H B

    2012-04-01

    Solution behaviour of hydroxyethyl cellulose (HEC) is reported in the polymer concentration range spanning over two decades (c=0.002-5% (w/v)). The results conclude the following: (i) dilute solution regime prevailed for c<0.2% (w/v), flexible HEC fibres of typical length ≈ 1 μm and persistence length ≈ 10 nm were found here, (ii) for 0.2w/v), a semidilute phase comprising soluble aggregates of hydrated HEC fibrils were observed with the material exhibiting viscoelastic behaviour and (iii) when 1w/v) the solution behaved with melt-like attributes with substantial embedded heterogeneity; viscous to elastic transition was observed in this region. Raman spectral, and DSC data indicated distinctive hydration of HEC fibres in the aforesaid concentration regimes. Cole-Cole plots revealed phase homogeneity and miscibility was limited to concentrations less than ~2% (w/v). For higher polymer concentrations, strong fibre-fibre interactions prevailed and samples became heterogeneous.

  4. Primary estimation of annual variation of water vapor in the Arctic Ocean between 70-80°N using shipborne GPS data based on kinematic precise point positioning

    NASA Astrophysics Data System (ADS)

    Luo, X.; Zhang, T.; Gao, J.; Wu, Z.

    2014-12-01

    The measurement of atmospheric water vapor (WV) content and variability is important for meteorological and climatological research. A technique for the remote sensing of atmospheric WV content using ground-based Global Positioning System (GPS)has become available, which can routinely achieve accuracies for integrated WV content of 1-2 kg/m2. Some experimental work has shown that the accuracy of WV measurements from a moving platform is comparable to that of (static) land-based receivers. Extending this technique into the marine environment on a moving platform would be greatly beneficial for many aspects of meteorological research, such as the calibration of satellite data, investigation of the air-sea interface, as well as forecasting and climatological studies. In this study, kinematic precise point positioning has been developed to investigate WV in the Arctic Ocean (70-80°N) and annual variations are obtained for 2008 and 2012 that are identical to those related to the enhanced greenhouse effect.

  5. Statistical media and process optimization for biotransformation of rice bran to vanillin using Pediococcus acidilactici.

    PubMed

    Kaur, Baljinder; Chakraborty, Debkumar

    2013-11-01

    An isolate of P. acidilactici capable of producing vanillin from rice bran was isolated from a milk product. Response Surface Methodology was employed for statistical media and process optimization for production of biovanillin. Statistical medium optimization was done in two steps involving Placket Burman Design and Central Composite Response Designs. The RSM optimized vanillin production medium consisted of 15% (w/v) rice bran, 0.5% (w/v) peptone, 0.1% (w/v) ammonium nitrate, 0.005% (w/v) ferulic acid, 0.005% (w/v) magnesium sulphate, and 0.1% (v/v) tween-80, pH 5.6, at a temperature of 37 degrees C under shaking conditions at 180 rpm. 1.269 g/L vanillin was obtained within 24 h of incubation in optimized culture medium. This is the first report indicating such a high vanillin yield obtained during biotransformation of ferulic acid to vanillin using a Pediococcal isolate.

  6. Enhanced production of bacterial cellulose by using Gluconacetobacter hansenii NCIM 2529 strain under shaking conditions.

    PubMed

    Mohite, Bhavna V; Salunke, Bipinchandra K; Patil, Satish V

    2013-03-01

    Bacterial cellulose (BC), a biopolymer, due to its unique properties is valuable for production of vital products in food, textile, medicine, and agriculture. In the present study, the optimal fermentation conditions for enhanced BC production by Gluconacetobacter hansenii NCIM 2529 were investigated under shaking conditions. The investigation on media components and culture parameters revealed that 2 % (w/v) sucrose as carbon source, 0.5 % (w/v) potassium nitrate as nitrogen source, 0.4 % (w/v) disodium phosphate as phosphate source, 0.04 % (w/v) magnesium sulfate, and 0.8 % (w/v) calcium chloride as trace elements, pH5.0, temperature 25 °C, and agitation speed 170 rpm with 6 days of fermentation period are optimal for maximum BC production. Production of BC using optimized media components and culture parameters was 1.66 times higher (5.0 g/l) than initial non optimized media (3.0 g/l). Fourier transform infrared spectroscopy spectrum and comparison with the available literature suggests that the produced component by G. hansenii in the present study is pure bacterial cellulose. The specific action of cellulase out of the investigated hydrolytic enzymes (cellulase, amylase, and protease) further confirmed purity of the produced BC. These findings give insight into conditions necessary for enhanced production of bacterial cellulose, which can be used for a variety of applications.

  7. Production of a bioemulsifier with potential application in the food industry.

    PubMed

    Campos, Jenyffer M; Stamford, Tânia L M; Sarubbo, Leonie A

    2014-03-01

    Biosurfactants are of considerable interest due to their biodegradability, low degree of toxicity, and diverse applications. However, the high production costs involved in the acquisition of biosurfactants underscore the need for optimization of the production process to enable viable application on an industrial scale. The aims of the present study were to select a species of Candida that produces a biosurfactant with the greatest emulsifying potential and to investigate the influence of components of the production medium and cultivation conditions. Candida utilis achieved the lowest surface tension (35.53 mN/m), best emulsification index (73%), and highest yield (12.52 g/l) in a medium containing waste canola frying oil as the carbon source and ammonium nitrate as the nitrogen source. The best combination of medium components and cultivation conditions was 6% (w/v) glucose, 6% (w/v) waste canola frying oil, 0.2% (w/v) ammonium nitrate, 0.3% (w/v) yeast extract, 150 rpm, 1% inoculum (w/v), and 88 h of fermentation. The greatest biosurfactant production and the lowest surface tension were achieved in the first 24 h of production, and the maximum biomass production was recorded at 72 h. The biosurfactant produced from C. utilis under the conditions investigated in the present study has a potential to be a bioemulsifier for application in the food industry.

  8. Ocular toxicity of benzalkonium chloride homologs compared with their mixtures.

    PubMed

    Okahara, Akihiko; Tanioka, Hidetoshi; Takada, Koichi; Kawazu, Kouichi

    2013-12-01

    This study was performed to assess the in vivo ocular toxicity of benzalkonium chloride (BAK) homologs compared with commercially available BAK (BAK mixture) and to assess the ocular toxicity of BAK homolog after repeated ocular application. Rabbit eyes were examined by ophthalmology and scanning electron microscopy (SEM) after 10 applications of BAK homologs with C12 (C12-BAK) and C14 (C14-BAK) alkyl chain lengths and a BAK mixture at concentrations of 0.001% (w/v), 0.003% (w/v), 0.005% (w/v), 0.01% (w/v) and 0.03% (w/v). The ocular toxicity of C12-BAK to rabbit eyes was examined by ophthalmology and histopathology after repeated ocular application for 39 weeks. In addition, the antimicrobial activities of C12-BAK and C14-BAK against A. niger, S. aureus and P. aeruginosa were assessed. Ocular toxicity of C12-BAK was less than those of the BAK mixture and C14-BAK. No ocular toxicity was noted after ocular application of 0.01% C12-BAK to rabbits for 39 weeks. C12-BAK showed antimicrobial activities at a concentration of 0.003%. These results suggest that the use of C12-BAK to replace BAK mixture as a preservative in ophthalmic solutions should be considered in order to reduce the incidence of the corneal epithelial cell injury induced clinically by BAK.

  9. Ocular Toxicity of Benzalkonium Chloride Homologs Compared with Their Mixtures

    PubMed Central

    Okahara, Akihiko; Tanioka, Hidetoshi; Takada, Koichi; Kawazu, Kouichi

    2013-01-01

    This study was performed to assess the in vivo ocular toxicity of benzalkonium chloride (BAK) homologs compared with commercially available BAK (BAK mixture) and to assess the ocular toxicity of BAK homolog after repeated ocular application. Rabbit eyes were examined by ophthalmology and scanning electron microscopy (SEM) after 10 applications of BAK homologs with C12 (C12-BAK) and C14 (C14-BAK) alkyl chain lengths and a BAK mixture at concentrations of 0.001% (w/v), 0.003% (w/v), 0.005% (w/v), 0.01% (w/v) and 0.03% (w/v). The ocular toxicity of C12-BAK to rabbit eyes was examined by ophthalmology and histopathology after repeated ocular application for 39 weeks. In addition, the antimicrobial activities of C12-BAK and C14-BAK against A. niger, S. aureus and P. aeruginosa were assessed. Ocular toxicity of C12-BAK was less than those of the BAK mixture and C14-BAK. No ocular toxicity was noted after ocular application of 0.01% C12-BAK to rabbits for 39 weeks. C12-BAK showed antimicrobial activities at a concentration of 0.003%. These results suggest that the use of C12-BAK to replace BAK mixture as a preservative in ophthalmic solutions should be considered in order to reduce the incidence of the corneal epithelial cell injury induced clinically by BAK. PMID:24526806

  10. Second-generation ethanol production from elephant grass at high total solids.

    PubMed

    Menegol, Daiane; Fontana, Roselei Claudete; Dillon, Aldo José Pinheiro; Camassola, Marli

    2016-07-01

    The enzymatic hydrolysis of Pennisetum purpureum (elephant grass) was evaluated at high total solid levels (from 4% to 20% (w/v)) in a concomitant ball milling treatment in a rotating hydrolysis reactor (RHR). The greatest glucose yield was 20.17% when 4% (w/v) untreated biomass was employed. When sugars obtained from enzymatic hydrolysis were submitted to fermentation with Saccharomyces cerevisiae, the greatest ethanol yield was 22.61% when 4% (w/v) untreated biomass was employed; however, the highest glucose concentration (12.47g/L) was obtaining using 20% (w/v) solids and highest ethanol concentration (6.1g/L) was obtained using 16% (w/v) solids. When elephant grass was hydrolyzed in the rotating hydrolysis reactor, ethanol production was about double that was produced when the biomass was hydrolyzed in a static reactor (SR). These data indicate that it is possible to produce ethanol from elephant grass when milling treatment and enzymatic hydrolysis are performed at the same time.

  11. Potential of carboxymethyl cellulose and γ-irradiation to maintain quality and control disease of peach fruit.

    PubMed

    Hussain, Peerzada R; Suradkar, Prashant P; Wani, Ali M; Dar, Mohd A

    2016-01-01

    Carboxymethyl cellulose (CMC) coatings alone and in combination with gamma irradiation were tested for maintaining the storage quality and control of post-harvest gray and black mold disease of peach. Matured green peaches were CMC coated at levels 0.5-1.0% (w/v) and gamma irradiated at 1.2 kGy. The treated fruit including control was stored under ambient (temperature 25 ± 2°C, RH 70%) and refrigerated (temperature 3 ± 1°C, RH 80%) conditions. In fruits treated with individual treatments of 1.0% (w/v) CMC; 1.2 kGy irradiation and combination of 1.0% (w/v) CMC and 1.2 kGy irradiation, no decay was recorded up to 6, 8 and 14 days of ambient storage. Irradiation alone at 1.2 kGy prevented the onset of disease incidence up to 4 days compared to 2 days by 1.0% (w/v) CMC coating following 30 days of refrigeration. Combination of CMC at 1.0% (w/v) and 1.2 kGy irradiation prevented disease incidence of peach up to 7 days during post-refrigerated storage at 25 ± 2°C, RH 70% following 30 days of refrigeration. Above combinatory treatment can be of great potential in facilitating the marketing of the fruit, thereby benefiting the growers.

  12. Follicle Depletion Provides a Permissive Environment for Ovarian Carcinogenesis

    PubMed Central

    Wang, Ying; Cai, Kathy Qi; Smith, Elizabeth R.; Yeasky, Toni M.; Moore, Robert; Ganjei-Azar, Parvin; Klein-Szanto, Andres J.; Godwin, Andrew K.; Hamilton, Thomas C.

    2016-01-01

    We modeled the etiology of postmenopausal biology on ovarian cancer risk using germ cell-deficient white-spotting variant (Wv) mice, incorporating oncogenic mutations. Ovarian cancer incidence is highest in peri- and postmenopausal women, and epidemiological studies have established the impact of reproductive factors on ovarian cancer risk. Menopause as a result of ovarian follicle depletion is thought to contribute to higher cancer risk. As a consequence of follicle depletion, female Wv mice develop ovarian tubular adenomas, a benign epithelial tumor corresponding to surface epithelial invaginations and papillomatosis frequently found in postmenopausal human ovaries. Lineage tracing using MISR2-Cre indicated that the tubular adenomas that developed in Wv mice were largely derived from the MISR2 lineage, which marked only a fraction of ovarian surface and oviduct epithelial cells in wild-type tissues. Deletion of p27, either heterozygous or homozygous, was able to convert the benign tubular adenomas into more proliferative tumors. Restricted deletion of p53 in Wv/Wv mice by either intrabursal injection of adenoviral Cre or inclusion of the MISR2-Cre transgene also resulted in augmented tumor growth. This finding suggests that follicle depletion provides a permissive ovarian environment for oncogenic transformation of epithelial cells, presenting a mechanism for the increased ovarian cancer risk in postmenopausal women. PMID:27354067

  13. Trans-ungual Delivery of AR-12, a Novel Antifungal Drug.

    PubMed

    Kushwaha, Avadhesh Singh; Sharma, P; Shivakumar, H N; Rappleye, C; Zukiwski, A; Proniuk, S; Murthy, S Narasimha

    2017-03-13

    AR-12 is a novel small molecule with broad spectrum antifungal activity. Recently, AR-12 was found to be highly active against Trichophyton rubrum, one of the predominantly responsible organisms that cause onychomycosis. The primary objective of this project was to investigate the ability of AR-12 to penetrate into and across the human nail plate followed by improving its trans-ungual permeation using different penetration enhancers. TranScreen-N™, a high throughput screening method was utilized to explore the potential nail penetration enhancers to facilitate the drug delivery through the nail. This screen demonstrated that dexpanthenol and PEG 400 were the most efficient enhancers. The in vitro permeation studies were performed across the human cadaver nail plates for 7 days with three AR-12 (5% w/v) formulations containing 10% w/v dexpanthenol (Formulation A), 10% w/v PEG 400 (Formulation B), and a combination of 10% w/v dexpanthenol + 10% w/v PEG 400 (Formulation C). The in vitro studies concluded that dexpanthenol and PEG 400 were able to deliver a significant amount of AR-12 into and across the nail plate that was found to be more than MIC 50 level of the drug.

  14. The Exploding Spark: Workplace Violence in an Infectious Disease Hospital—A Longitudinal Study

    PubMed Central

    2013-01-01

    Objectives. Workplace violence (WV) is an important occupational hazard for healthcare workers (HCWs). Methods. A longitudinal study was carried out on HCWs from an infectious disease hospital. Work-related stress, anxiety, and depression were measured at baseline in 2003, and they were reassessed in 2005, along with the assaults that occurred in the previous year. Results. One-year prevalences of 6.2% and 13.9% were reported for physical and verbal aggressions, respectively. Perpetrators were mainly patients. The professional groups most frequently attacked were physicians, followed by nurses. Workers with job strain at baseline had a significant risk of being subject to aggression (OR 7.7; CI 95%, 3.3–17.9) in the following year. The relationship between job strain and subsequent WV remained significant even after correction for anxiety, depression, and other confounders. Conversely, experiencing WV was associated with a high risk of job strain and effort-reward imbalance in the following year. The final levels of anxiety and depression were predicted using regression models that included physical aggression among predictive variables. Conclusions. WV is the spark that sets off a problematic work situation. Effective prevention of WV can only be achieved within the framework of an overall improvement in the quality of work. PMID:23936789

  15. Identification of a novel multiple kinase inhibitor with potent antiviral activity against influenza virus by reducing viral polymerase activity.

    PubMed

    Sasaki, Yutaka; Kakisaka, Michinori; Chutiwitoonchai, Nopporn; Tajima, Shigeru; Hikono, Hirokazu; Saito, Takehiko; Aida, Yoko

    2014-07-18

    Neuraminidase inhibitors are the only currently available influenza treatment, although resistant viruses to these drugs have already been reported. Thus, new antiviral drugs with novel mechanisms of action are urgently required. In this study, we identified a novel antiviral compound, WV970, through cell-based screening of a 50,000 compound library and subsequent lead optimization. This compound exhibited potent antiviral activity with nanomolar IC50 values against both influenza A and B viruses but not non-influenza RNA viruses. Time-of-addition and indirect immunofluorescence assays indicated that WV970 acted at an early stage of the influenza life cycle, but likely after nuclear entry of viral ribonucleoprotein (vRNP). Further analyses of viral RNA expression and viral polymerase activity indicated that WV970 inhibited vRNP-mediated viral genome replication and transcription. Finally, structure-based virtual screening and comprehensive human kinome screening were used to demonstrate that WV970 acts as a multiple kinase inhibitor, many of which are associated with influenza virus replication. Collectively, these results strongly suggest that WV970 is a promising anti-influenza drug candidate and that several kinases associated with viral replication are promising drug targets.

  16. Inhibitory responses mediated by vagal nerve stimulation are diminished in stomachs of mice with reduced intramuscular interstitial cells of Cajal

    PubMed Central

    Beckett, Elizabeth A. H.; Sanders, Kenton M.; Ward, Sean M.

    2017-01-01

    Intramuscular interstitial cells of Cajal (ICC-IM) are closely associated with enteric motor nerve terminals and electrically coupled to smooth muscle cells within the gastric musculature. Previous studies investigating the role of ICC-IM in motor neurotransmission have used indiscriminate electric field stimulation of neural elements within the gastric wall. To determine the role of ICC-IM in transduction of vagally-mediated motor input to gastric muscles electrical and mechanical responses to selective electrical vagal stimulation (EVS) were recorded from gastric fundus and antral regions of wild type and W/WV mice, which lack most ICC-IM. EVS evoked inhibitory junction potentials (IJPs) in wild type muscles that were attenuated or abolished by L-NNA. IJPs were rarely evoked in W/WV muscles by EVS, and not affected by L-NNA. EVS evoked relaxation of wild type stomachs, but the predominant response of W/WV stomachs was contraction. EVS applied after pre-contraction with bethanechol caused relaxation of wild type gastric tissues and these were inhibited by the nitric oxide synthase inhibitor L-NNA. Relaxation responses were of smaller amplitude in W/WV muscles and L-NNA did not attenuate relaxation responses in W/WV fundus muscles. These data suggest an important role for ICC-IM in vagally-mediated nitrergic relaxation in the proximal and distal stomach. PMID:28317837

  17. Modeling the air-soil transport pathway of perfluorooctanoic acid in the mid-Ohio Valley using linked air dispersion and vadose zone models

    NASA Astrophysics Data System (ADS)

    Shin, Hyeong-Moo; Ryan, P. Barry; Vieira, Verónica M.; Bartell, Scott M.

    2012-05-01

    As part of an extensive modeling effort on the air-soil-groundwater transport pathway of perfluorooctanoic acid (PFOA), this study was designed to compare the performance of different air dispersion modeling systems (AERMOD vs. ISCST3), and different approaches to handling incomplete meteorological data using a data set with substantial soil measurements and a well characterized point source for air emissions. Two of the most commonly used EPA air dispersion models, AERMOD and ISCST3, were linked with the EPA vadose zone model PRZM-3. Predicted deposition rates from the air dispersion model were used as input values for the vadose zone model to estimate soil concentrations of PFOA at different depths. We applied 34 years of meteorological data including hourly surface measurements from Parkersburg Airport and 5 years of onsite wind direction and speed to the air dispersion models. We compared offsite measured soil concentrations to predictions made for the corresponding sampling depths, focusing on soil rather than air measurements because the offsite soil samples were less likely to be influenced by short-term variability in emission rates and meteorological conditions. PFOA concentrations in surface soil (0-30 cm depth) were under-predicted and those in subsurface soil (>30 cm depth) were over-predicted compared to observed concentrations by both linked air and vadose zone model. Overall, the simulated values from the linked modeling system were positively correlated with those observed in surface soil (Spearman's rho, Rsp = 0.59-0.70) and subsurface soil (Rsp = 0.46-0.48). This approach provides a useful modeling scheme for similar exposure and risk analyses where the air-soil-groundwater transport is a primary contamination pathway.

  18. A fast radiative transfer model for the assimilation of water vapor radiances from the Kalpana very high resolution radiometer

    NASA Astrophysics Data System (ADS)

    Singh, Randhir; Rayer, Peter; Saunders, Roger; Migliorini, Stefano; Brugge, Roger; O'Neill, Alan

    2009-04-01

    A fast radiative transfer model (RTM) to compute emitted infrared radiances for a very high resolution radiometer (VHRR), onboard the operational Indian geostationary satellite Kalpana has been developed and verified. This work is a step towards the assimilation of Kalpana water vapor (WV) radiances into numerical weather prediction models. The fast RTM uses a regression-based approach to parameterize channel-specific convolved level to space transmittances. A comparison between the fast RTM and the line-by-line RTM demonstrated that the fast RTM can simulate line-by-line radiances for the Kalpana WV channel to an accuracy better than the instrument noise, while offering more rapid radiance calculations. A comparison of clear sky radiances of the Kalpana WV channel with the ECMWF model first guess radiances is also presented, aiming to demonstrate the fast RTM performance with the real observations. In order to assimilate the radiances from Kalpana, a simple scheme for bias correction has been suggested.

  19. Developing a Dissemination Model to Improve Intervention Reach among West Virginia Youth Smokers.

    PubMed

    Horn, Kimberly; Jarrett, Traci; Anesetti-Rothermel, Andrew; O'Hara Tompkins, Nancy; Dino, Geri

    2014-01-01

    The not-on-tobacco program is an evidence-based teen smoking cessation program adopted by the American Lung Association (ALA). Although widely disseminated nationally via ALA Master Trainers, in recent years, adoption and implementation of the N-O-T program in West Virginia (WV) has slowed. WV, unfortunately, has one of the highest smoking rates in the US. Although it is a goal of public health science, dissemination of evidence-based interventions is woefully understudied. The present manuscript reviews a theoretical model of dissemination of the not-on-tobacco program in WV. Based on social marketing, diffusion of innovations, and social cognitive theories, the nine-phase model incorporates elements of infrastructure development, accountability, training, delivery, incentives, and communication. The model components as well as preliminary lessons learned from initial implementation are discussed.

  20. Preliminary geothermal investigations in West Virginia

    SciTech Connect

    Hendry, R.; Hilfiker, K.; Hodge, D.; Morgan, P.; Swanberg, C.

    1981-10-01

    Deep sedimentary basins and warm spring systems are potential geothermal resources in West Virginia. A temperature gradient map based on 800 BHT for WV shows that variation of temperature gradients trend NE parallel to regional structure. Highest temperature gradient values of about 28/sup 0/C/km occur in the eastcentral WV and the lowest gradients (18/sup 0/C/km) are found over the Rome trough. Results of groundwater geochemistry indicate that the warm springs circulate in very shallow aquifers and are subject to seasonal temperature fluctuations. Silica heat flow data in WV varies from about .89 to 1.4 HFU and generally increases towards the west. Bouguer, magnetic and temperature gradient profiles suggest that an ancient rift transects the State and is the site of several deep sedimentary basins.

  1. Developing a Dissemination Model to Improve Intervention Reach among West Virginia Youth Smokers

    PubMed Central

    Horn, Kimberly; Jarrett, Traci; Anesetti-Rothermel, Andrew; O’Hara Tompkins, Nancy; Dino, Geri

    2014-01-01

    The not-on-tobacco program is an evidence-based teen smoking cessation program adopted by the American Lung Association (ALA). Although widely disseminated nationally via ALA Master Trainers, in recent years, adoption and implementation of the N-O-T program in West Virginia (WV) has slowed. WV, unfortunately, has one of the highest smoking rates in the US. Although it is a goal of public health science, dissemination of evidence-based interventions is woefully understudied. The present manuscript reviews a theoretical model of dissemination of the not-on-tobacco program in WV. Based on social marketing, diffusion of innovations, and social cognitive theories, the nine-phase model incorporates elements of infrastructure development, accountability, training, delivery, incentives, and communication. The model components as well as preliminary lessons learned from initial implementation are discussed. PMID:25136547

  2. A demonstration of the susceptibility of clinical isolates obtained from cystic fibrosis patients to manuka honey.

    PubMed

    Jenkins, Rowena; Wootton, Mandy; Howe, Robin; Cooper, Rose

    2015-05-01

    Pseudomonas and Burkholderia pose a significant health threat to people with chronic respiratory conditions; the resistance inherent in these bacteria indicates that new antimicrobial strategies are required. Susceptibility of 56 strains of P. aeruginosa and 55 strains of Burkholderia to manuka honey, tobramycin and colistin using microbroth dilution and E strip was determined. MICs of antibiotics with honey were determined to search for synergistic combinations against two representative strains of each genus. All strains exhibited susceptibility to honey ≤10 % (w/v); mean susceptibility of Burkholderia (4.6 % w/v) was lower than P. aeruginosa (7.3 % w/v). Synergistic or additive combinations were found with all four strains tested. Combinations of manuka honey with antibiotics can be used to lower the MIC need to successfully inhibit both P. aeruginosa and B. cepacia. The use of honey as a combination agent may be possible for the management of P. aeruginosa and B. cepacia.

  3. Wake modeling in complex terrain using a hybrid Eulerian-Lagrangian Split Solver

    NASA Astrophysics Data System (ADS)

    Fuchs, Franz G.; Rasheed, Adil; Tabib, Mandar; Fonn, Eivind

    2016-09-01

    Wake vortices (WVs) generated by aircraft are a source of risk to the following aircraft. The probability of WV related accidents increases in the vicinity of airport runways due to the shorter time of recovery after a WV encounter. Hence, solutions that can reduce the risk of WV encounters are needed to ensure increased flight safety. In this work we propose an interesting approach to model such wake vortices in real time using a hybrid Eulerian- Lagrangian approach. We derive an appropriate mathematical model, and show a comparison of the different types of solvers. We will conclude with a real life application of the methodology by simulating how wake vortices left behind by an aircraft at the Vffirnes airport in Norway get transported and decay under the influence of a background wind and turbulence field. Although the work demonstrates the application in an aviation context the same approach can be used in a wind energy context.

  4. Bioleaching of rare earth and radioactive elements from red mud using Penicillium tricolor RM-10.

    PubMed

    Qu, Yang; Lian, Bin

    2013-05-01

    The aim of this work is to investigate biological leaching of rare earth elements (REEs) and radioactive elements from red mud, and to evaluate the radioactivity of the bioleached red mud used for construction materials. A filamentous, acid-producing fungi named RM-10, identified as Penicillium tricolor, is isolated from red mud. In our bioleaching experiments by using RM-10, a total concentration of 2% (w/v) red mud under one-step bioleaching process was generally found to give the maximum leaching ratios of the REEs and radioactive elements. However, the highest extraction yields are achieved under two-step bioleaching process at 10% (w/v) pulp density. At pulp densities of 2% and 5% (w/v), red mud processed under both one- and two-step bioleaching can meet the radioactivity regulations in China.

  5. Ingestion toxicity of three Lamiaceae essential oils incorporated in protein baits against the olive fruit fly, Bactrocera oleae (Rossi) (Diptera Tephritidae).

    PubMed

    Canale, Angelo; Benelli, Giovanni; Conti, Barbara; Lenzi, Gabriele; Flamini, Guido; Francini, Alessandra; Cioni, Pier Luigi

    2013-01-01

    The ingestion toxicity of three Lamiaceae essential oils (EOs) - Hyptis suaveolens, Rosmarinus officinalis and Lavandula angustifolia - incorporated in protein baits was evaluated against Bactrocera oleae, a worldwide pest of olive fruits. In laboratory conditions, all the tested EOs showed dose-dependent toxicity on B. oleae, with mortality rates ranging from 12% (EO concentration: 0.01% w:v) to 100% (EO concentration: 1.75% w:v). Semi-field results highlighted the toxicity of L. angustifolia and H. suaveolens EOs, which exerted more than 60% of flies mortality at a concentration of 1.75% (w:v). Gas Chromatography-Electron Impact Mass Spectrometry analyses of the three EOs showed that H. suaveolens EO was dominated by monoterpene and sesquiterpene hydrocarbons. Oxygenated monoterpenes were the main chemical class in R. officinalis and L. angustifolia EOs. Further research is needed to evaluate the efficacy of these EOs plus food bait against the olive fruit fly in the open field.

  6. Synergistic Effects of Nanochitin on Inhibition of Tobacco Root Rot Disease.

    PubMed

    Zhou, Yang; Jiang, Shijun; Jiao, Yongji; Wang, Hezhong

    2017-02-22

    Nanomaterials have great potential for use in various fields, due to their unique properties. In order to explore the bioactivity of nanochitin on tobacco, the effects of nanochitin suspensions on tobacco seed germination, seedling growth, and synergistic effects with fungicides were studied in indoor and field trials. Results showed that 0.004% (w/v) of nanochitin improved tobacco seed germination and shortened mean time to germination significantly; 0.005% (w/v) of nanochitin increased tobacco stem length, stem girth, leaf number and leaf area, and 0.001% (w/v) of nanochitin had synergistic effects on inhibition of tobacco root rot when mixed with metalaxyl mancozeb and thiophanate methyl fungicides. This indicates that nanochitin suspensions have a strong potential to protect tobacco from tobacco root rot diseases and reduce the use of chemical fungicides in tobacco plantations.

  7. Efficient butanol recovery from acetone-butanol-ethanol fermentation cultures grown on sweet sorghum juice by pervaporation using silicalite-1 membrane.

    PubMed

    Kanemoto, Miho; Negishi, Hideyuki; Sakaki, Keiji; Ikegami, Toru; Chohnan, Shigeru; Nitta, Youji; Kurusu, Yasurou; Ohta, Hiroyuki

    2016-06-01

    We investigated butanol recovery by pervaporation separation, using a silicalite-1 membrane, from batch cultures of butanol-producing Clostridium beijerinckii SBP2 grown on sweet sorghum juice as a fermentation medium. The pervaporation system yielded 73% (w/v) butanol from intact feed cultures containing 1% (w/v) butanol, and had a butanol permeation flux of 11 g m(-2) h(-1). Upon neutralization and activated charcoal treatment of the feed cultures, butanol yield and total flux increased to 82% (w/v) and 40 g m(-2) h(-1), respectively. This system is applicable to refining processes for practical biobutanol production from a promising energy crop, sweet sorghum.

  8. Laboratory testing of West Valley reference 6 glass

    SciTech Connect

    Ebert, W.L.

    1995-07-01

    A series of laboratory tests is being conducted to characterize the corrosion of West Valley reference 6 glass (WV6) and to provide parametric values for modeling its long-term durability. Models require measurement of the corrosion rate in the absence of corrosion products and in fluids that are {open_quotes}saturated{close_quotes} with corrosion products, and the identification of alteration phases. Corrosion rates in dilute and saturated conditions were measured using MCC-1 and PCT tests, respectively. Vapor hydration tests were performed to generate secondary phases. The PCT tests show the WV6 glass to be more durable than SRL EA, SRL 202, and HW-39-1 glasses. Vapor hydration tests show weeksite (a uranyl silicate), a potassium-bearing zeolite, analcime, potassium feldspar, a calcium silicate phase, and lithium phosphate to form as WV6 glass corrodes. Test results are presented and their relevance to long-term performance discussed.

  9. Mast cells play a key role in Th2 cytokine-dependent asthma model through production of adhesion molecules by liberation of TNF-α.

    PubMed

    Chai, Ok Hee; Han, Eui-Hyeog; Lee, Hern-Ku; Song, Chang Ho

    2011-01-31

    Mast cells are well recognized as key cells in allergic reactions, such as asthma and allergic airway diseases. However, the effects of mast cells and TNF-α on T-helper type 2 (Th2) cytokine-dependent asthma are not clearly understood. Therefore, an aim of this study was to investigate the role of mast cells on Th2 cytokine-dependent airway hyperresponsiveness and inflammation. We used genetically mast cell-deficient WBB6F1/J-Kitw/Kitw-v (W/Wv), congenic normal WBB6F1/J-Kit+/Kit+ (+/+), and mast cell-reconstituted W/Wv mouse models of allergic asthma to investigate the role of mast cells in Th2 cytokine-dependent asthma induced by ovalbumin (OVA). And we investigated whether the intratracheal injection of TNF-α directly induce the expression of ICAM-1 and VCAM-1 in W/Wv mice. This study, with OVA-sensitized and OVA-challenged mice, revealed the following typical histopathologic features of allergic diseases: increased inflammatory cells of the airway, airway hyperresponsiveness, and increased levels of TNF-α, intercellular adhesion molecule (ICAM)-1, and vascular cellular adhesion molecule (VCAM)-1. However, the histopathologic features and levels of ICAM-1 and VCAM-1 proteins in W/Wv mice after OVA challenges were significantly inhibited. Moreover, mast cell-reconstituted W/Wv mice showed restoration of histopathologic features and recovery of ICAM-1 and VCAM-1 protein levels that were similar to those found in +/+ mice. Intratracheal administration of TNF-α resulted in increased ICAM-1 and VCAM-1 protein levels in W/Wv mice. These results suggest that mast cells play a key role in a Th2 cytokine-dependent asthma model through production of adhesion molecules, including ICAM-1 and VCAM-1, by liberation of TNF-α.

  10. Quality of root canal fillings using three gutta-percha obturation techniques

    PubMed Central

    Ho, Edith Siu Shan; Chang, Jeffrey Wen Wei

    2016-01-01

    Objectives The goal of this study was to compare the density of gutta-percha root fillings obturated with the following techniques: cold lateral (CL) compaction, ultrasonic lateral (UL) compaction, and warm vertical (WV) compaction. Materials and Methods Thirty-three extracted mandibular first molars, with two separate mesial canals in each, were selected. After instrumentation, the canals were stratified into three groups based on canal length and curvature, and underwent obturation with one of the techniques. No sealer was used in order to avoid masking any voids. The teeth were imaged pre- and post-obturation using micro-computed tomography. The reconstructed three-dimensional images were analyzed volumetrically to determine the amount of gutta-percha present in every 2 mm segment of the canal. P values < 0.05 were considered to indicate statistical significance. Results The overall mean volume fraction of gutta-percha was 68.51 ± 6.75% for CL, 86.56 ± 5.00% for UL, and 88.91 ± 5.16% for WV. Significant differences were found between CL and UL and between CL and WV (p < 0.05), but not between UL and WV (p = 0.526). The gutta-percha density of the roots treated with WV and UL increased towards the coronal aspect, but this trend was not noted in the CL group. Conclusions WV compaction and UL compaction produced a significantly denser gutta-percha root filling than CL compaction. The density of gutta-percha was observed to increase towards the coronal aspect when the former two techniques were used. PMID:26877987

  11. A process for the production of ectoine and poly(3-hydroxybutyrate) by Halomonas boliviensis.

    PubMed

    Guzmán, Héctor; Van-Thuoc, Doan; Martín, Javier; Hatti-Kaul, Rajni; Quillaguamán, Jorge

    2009-10-01

    The paper reports a study involving the use of Halomonas boliviensis, a moderate halophile, for co-production of compatible solute ectoine and biopolyester poly(3-hydroxybutyrate) (PHB) in a process comprising two fed-batch cultures. Initial investigations on the growth of the organism in a medium with varying NaCl concentrations showed the highest level of intracellular accumulation of ectoine (0.74 g L(-1)) at 10-15% (w/v) NaCl, while at 15% (w/v) NaCl, the presence of hydroxyectoine (50 mg L(-1)) was also noted. On the other hand, the maximum cell dry weight and PHB concentration of 10 and 5.8 g L(-1), respectively, were obtained at 5-7.5% (w/v) NaCl. A process comprising two fed-batch cultivations was developed-the first culture aimed at obtaining high cell mass and the second for achieving high yields of ectoine and PHB. In the first fed-batch culture, H. boliviensis was grown in a medium with 4.5% (w/v) NaCl and sufficient levels of monosodium glutamate, NH (4) (+) , and PO (4) (3-) . In the second fed-batch culture, the NaCl concentration was increased to 7.5% (w/v) to trigger ectoine synthesis, while nitrogen and phosphorus sources were fed only during the first 3 h and then stopped to favor PHB accumulation. The process resulted in PHB yield of 68.5 wt.% of cell dry weight and volumetric productivity of about 1 g L(-1) h(-1) and ectoine concentration, content, and volumetric productivity of 4.3 g L(-1), 7.2 wt.%, and 2.8 g L(-1) day(-1), respectively. At salt concentration of 12.5% (w/v) during the second cultivation, the ectoine content was increased to 17 wt.% and productivity to 3.4 g L(-1) day(-1).

  12. Are Interstitial Cells of Cajal Involved in Mechanical Stress-Induced Gene Expression and Impairment of Smooth Muscle Contractility in Bowel Obstruction?

    PubMed Central

    Wu, Chester C.; Lin, You-Min; Gao, Jerry; Winston, John H.; Cheng, Leo K.; Shi, Xuan-Zheng

    2013-01-01

    Background and Aims The network of interstitial cells of Cajal (ICC) is altered in obstructive bowel disorders (OBD). However, whether alteration in ICC network is a cause or consequence of OBD remains unknown. This study tested the hypothesis that mechanical dilation in obstruction disrupts the ICC network and that ICC do not mediate mechanotranscription of COX-2 and impairment of smooth muscle contractility in obstruction. Methods Medical-grade silicon bands were wrapped around the distal colon to induce partial obstruction in wild-type and ICC deficient (W/Wv) mice. Results In wild-type mice, colon obstruction led to time-dependent alterations of the ICC network in the proximal colon segment. Although unaffected on days 1 and 3, the ICC density decreased markedly and the network was disrupted on day 7 of obstruction. COX-2 expression increased, and circular muscle contractility decreased significantly in the segment proximal to obstruction. In W/Wv control mice, COX-2 mRNA level was 4.0 (±1.1)-fold higher (n=4) and circular muscle contractility was lower than in wild-type control mice. Obstruction further increased COX-2 mRNA level in W/Wv mice to 7.2 (±1.0)-fold vs. W/Wv controls [28.8 (±4.1)-fold vs. wild-type controls] on day 3. Obstruction further suppressed smooth muscle contractility in W/Wv mice. However, daily administration of COX-2 inhibitor NS-398 significantly improved muscle contractility in both W/Wv sham and obstruction mice. Conclusions Lumen dilation disrupts the ICC network. ICC deficiency has limited effect on stretch-induced expression of COX-2 and suppression of smooth muscle contractility in obstruction. Rather, stretch-induced COX-2 plays a critical role in motility dysfunction in partial colon obstruction. PMID:24098782

  13. Effect of pH and lactic or acetic acid on ethanol productivity by Saccharomyces cerevisiae in corn mash.

    PubMed

    Graves, Tara; Narendranath, Neelakantam V; Dawson, Karl; Power, Ronan

    2006-06-01

    The effects of lactic and acetic acids on ethanol production by Saccharomyces cerevisiae in corn mash, as influenced by pH and dissolved solids concentration, were examined. The lactic and acetic acid concentrations utilized were 0, 0.5, 1.0, 2.0, 3.0 and 4.0% w/v, and 0, 0.1, 0.2, 0.4, 0.8 and 1.6% w/v, respectively. Corn mashes (20, 25 and 30% dry solids) were adjusted to the following pH levels after lactic or acetic acid addition: 4.0, 4.5, 5.0 or 5.5 prior to yeast inoculation. Lactic acid did not completely inhibit ethanol production by the yeast. However, lactic acid at 4% w/v decreased (P<0.05) final ethanol concentration in all mashes at all pH levels. In 30% solids mash set at pH < or =5, lactic acid at 3% w/v reduced (P<0.05) ethanol production. In contrast, inhibition by acetic acid increased as the concentration of solids in the mash increased and the pH of the medium declined. Ethanol production was completely inhibited in all mashes set at pH 4 in the presence of acetic acid at concentrations > or =0.8% w/v. In 30% solids mash set at pH 4, final ethanol levels decreased (P<0.01) with only 0.1% w/v acetic acid. These results suggest that the inhibitory effects of lactic acid and acetic acid on ethanol production in corn mash fermentation when set at a pH of 5.0-5.5 are not as great as that reported thus far using laboratory media.

  14. Investigating the effect of Aloe vera gel on the buccal permeability of didanosine.

    PubMed

    Ojewole, Elizabeth; Mackraj, Irene; Akhundov, Kamil; Hamman, Josias; Viljoen, Alvaro; Olivier, Eugene; Wesley-Smith, James; Govender, Thirumala

    2012-03-01

    The buccal mucosal route offers several advantages but the delivery of certain drugs can be limited by low membrane permeability. This study investigated the buccal permeability properties of didanosine (ddI) and assessed the potential of Aloe vera gel (AVgel) as a novel buccal permeation enhancer. Permeation studies were performed using Franz diffusion cells, and the drug was quantified by UV spectroscopy. Histomorphological evaluations were undertaken using light and transmission electron microscopy. The permeability of ddI was concentration-dependent, and it did not have any adverse effects on the buccal mucosae. A linear relationship (R² = 0.9557) between the concentrations and flux indicated passive diffusion as the mechanism of drug transport. AVgel at concentrations of 0.25 to 2 %w/v enhanced ddI permeability with enhancement ratios from 5.09 (0.25 %w/v) to 11.78 (2 %w/v) but decreased permeability at 4 and 6 %w/v. Ultrastructural analysis of the buccal mucosae treated with phosphate buffer saline pH 7.4 (PBS), ddI/PBS, and ddI/PBS/AVgel 0.5 %w/v showed cells with normal plasmalemma, well-developed cristae, and nuclei with regular nuclear envelopes. However, cells from 1, 2, and 6 %w/v AVgel-treated mucosae showed irregular nuclear outlines, increased intercellular spacing, and plasmalemma crenulations. This study demonstrates the potential of AVgel as a buccal permeation enhancer for ddI to improve anti-HIV and AIDS therapy.

  15. Evaluating the robustness of models developed from field spectral data in predicting African grass foliar nitrogen concentration using WorldView-2 image as an independent test dataset

    NASA Astrophysics Data System (ADS)

    Mutanga, Onisimo; Adam, Elhadi; Adjorlolo, Clement; Abdel-Rahman, Elfatih M.

    2015-02-01

    In this paper, we evaluate the extent to which the resampled field spectra compare with the actual image spectra of the new generation multispectral WorldView-2 (WV-2) satellite. This was achieved by developing models from resampled field spectra data and testing them on an actual WV-2 image of the study area. We evaluated the performance of reflectance ratios (RI), normalized difference indices (NDI) and random forest (RF) regression model in predicting foliar nitrogen concentration in a grassland environment. The field measured spectra were used to calibrate the RF model using a randomly selected training (n = 70%) nitrogen data set. The model developed from the field spectra resampled to WV-2 wavebands was validated on an independent field spectral test dataset as well as on the actual WV-2 image of the same area (n = 30%, bootstrapped a 100 times). The results show that the model developed using RI could predict nitrogen with a mean R2 of 0.74 and 0.65 on an independent field spectral test data set and on the actual WV-2 image, respectively. The root mean square error of prediction (RMSE %) was 0.17 and 0.22 for the field test data set and the WV-2 image, respectively. Results provide an insight on the magnitude of errors that are expected when up-scaling field spectral models to airborne or satellite image data. The prediction also indicates the unceasing relevance of field spectroscopy studies to better understand the spectral models critical for vegetation quality assessment.

  16. Efficacy of Gamma-glutamylcysteine (GGC) in Ischemia-reperfusion Injury

    DTIC Science & Technology

    2012-07-01

    suspended and lysed with a hypotonic buffer and 10% (w/v) Nonidet P - 40 . After spinning, the cell pellet was re-lysed and centrifuged for 10 min at 4 C...lysed with hypotonic buffer and 10% (w/v) Nonidet P - 40 . After spinning, the cell pellet was re-lysed and centrifuged for 10 min at 4 °C and 14000×g...Mil Med 149: 55‐62. Liu, L‐M, Hu, D‐Y, Chen, H‐S, and Hu, P ‐H. 2004. The effect of different volumes of fluid resuscitation on traumatic

  17. Rapid Spectrophotometric Assay of Serum Iron

    DTIC Science & Technology

    1990-01-01

    conditions. Materials and methods MateriaLs Most of the chemicals used were purchased as iron -free reagents (G. Frederick Smith Co.. Columbus. OH). Water...Bedford. MA). The iron -releasing reagent was made to contain 0.5r Tween-20 (v/v), 0.55 thiourea (w/v) and 0.1% ascorbic acid (w/v). all dissolved in 0.1... reagent and 0.2 ml color-develop- ment reagent . A serum blank was prepared by adding to another test tube, 0.1 ml serum, 0.7 ml iron -releasing reagent and

  18. In-situ photopolymerization of monodisperse and discoid oxidized methacrylated alginate microgels in a microfluidic channel

    DOE PAGES

    Wang, Shuo; Jeon, Oju; Shankles, Peter G.; ...

    2016-02-03

    Here, we present a simple microfluidic technique to in-situ photopolymerize (by 365 nm ultraviolet) monodisperse oxidized methacrylated alginate (OMA) microgels using a photoinitiator (VA-086). By this technique, we generated monodisperse spherical OMA beads and discoid non-spherical beads with better shape consistency than ionic crosslinking methods do. We found that a high monomer concentration (8 w/v %), a high photoinitiator concentration (1.5 w/v %) and absence of oxygen are critical factors to cure OMA microgels. This photopolymerizing method is an alternative to current methods to form alginate microgels and is a simpler approach to generate non-spherical alginate microgels.

  19. In-situ photopolymerization of monodisperse and discoid oxidized methacrylated alginate microgels in a microfluidic channel

    SciTech Connect

    Wang, Shuo; Jeon, Oju; Shankles, Peter G.; Liu, Yuan; Alsberg, Eben; Retterer, Scott T.; Lee, Bruce P.; Choi, Chang Kyoung

    2016-02-03

    Here, we present a simple microfluidic technique to in-situ photopolymerize (by 365 nm ultraviolet) monodisperse oxidized methacrylated alginate (OMA) microgels using a photoinitiator (VA-086). By this technique, we generated monodisperse spherical OMA beads and discoid non-spherical beads with better shape consistency than ionic crosslinking methods do. We found that a high monomer concentration (8 w/v %), a high photoinitiator concentration (1.5 w/v %) and absence of oxygen are critical factors to cure OMA microgels. This photopolymerizing method is an alternative to current methods to form alginate microgels and is a simpler approach to generate non-spherical alginate microgels.

  20. Template Synthesized Nanoscopic Gold Particles: Optical Spectra and the Effects of Particle Size and Shape

    DTIC Science & Technology

    1994-01-25

    template membranes were prepared by anodization of aluminum (Aldrich 2mm plate 99.999% purity) in aqueous oxalic acid media. Anodization of aluminum to...prepared membrar .,L pores of four different diameters. Anodization at 40 V and 50 V in 4% (w/v) oxalic acid yielded membranes with pore diameters of...approximately 60 nm and 75 nm respectively. Anodization at 70 V and 90 V in 2% (w/v) oxalic acid produced pores with diameters of approximately 86 nm and

  1. Immunochemical Identification of Newcastle Disease Virus Structural Proteins,

    DTIC Science & Technology

    1984-01-01

    of 30% (w/v) acrylamide (Biorad Laboratories, Mississauga, Ontario) and 0.8% (w/v) N,N- methylene -bis- acrylamide (Biorad Laboratories). The gels were...Sigma Chemical Company)), 10% glycerol and 10 pl of 0.1% bromophenol blue (Biorad Laboratories) as the tracking dye. Reduced samples contained the...current of 15 mA/gel, until the bromsphenol blue marker entered the separating gel (about 1 h), at which time the current was increased to 30 mA/gel until

  2. Electrophoretic Characteristics of Outer Membrane Proteins of Neisseria meningitidis,

    DTIC Science & Technology

    1987-07-01

    prepared from a monomer stock of 3007o (w/v) acrylamide (Sigma Chemical Company, St. Louis, Missouri) and 0.8% (w/v) N,N- methylene -bis-acrylamide...buffer containing 0.05 M Tris-HCl (pH 6.8), 10% SDS, 10% glycerol, a pinch of bromophenol blue , and 17o 2-mercaptoethanol, such that all aliquots...Brilliant Blue /Silver Stain: Upon completion of electrophoresis, gels were stained for I h in a solution of Coomassie brilliant blue R250 (Sigma

  3. Mobility shift detection of phosphorylation on large proteins using a Phos-tag SDS-PAGE gel strengthened with agarose.

    PubMed

    Kinoshita, Eiji; Kinoshita-Kikuta, Emiko; Ujihara, Hiromi; Koike, Tohru

    2009-08-01

    We describe a novel technique of phosphate-affinity SDS-PAGE using Phos-tag to analyze large phosphoproteins with molecular masses of more than 200 kDa. The protein phosphoisotypes were clearly separated as up-shifted migration bands in a 3% w/v polyacrylamide gel containing 20 microM Phos-tag and 0.5% w/v agarose. In subsequent immunoblotting, the procedure permitted the determination of the phosphoisotypes of high-molecular-mass proteins, such as mTOR (289 kDa), ATM kinase (350 kDa), and 53BP1 (213 kDa).

  4. Preclinical Toxicology of New Drugs

    DTIC Science & Technology

    1988-07-31

    v/v) Tween 80 were conducted in Fischer 344 rats. The dose ranges encom- passed 66 mg/kg to 1400 mg/kg. G-8740-1400 Acute (LOSO) Intraperitoneal...1% (w/v) methylcellulose/O.4% (v/v) Tween 80 were conducted in Fischer 344 rats. The dose ranges encom- passed 0 mg/kg (vehicle control) to 320 mg/kg...suspended in a vehicle of 1% (w/v) methylcellulose/O.4% (v/v) Tween 80 were conducted in B6C3F1 mice. The dose range encompassed 62.5 mg/kg to 1000 mg

  5. Microbial killing activity of peracetic acid.

    PubMed

    Thamlikitkul, V; Trakulsomboon, S; Louisirirotchanakul, S; Chaiprasert, A; Foongladda, S; Thipsuvan, K; Arjratanakool, W; Kunyok, R; Wasi, C; Santiprasitkul, S; Danchaivijitr, S

    2001-10-01

    In vitro killing activity of peracetic acid (Perasafe) at a concentration of 0.26 per cent w/v was tested against Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Salmonella paratyphi A, Acinetobacter baumannii, Sternotrophomonas maltophilia, Enterococcus faecium, Enterococcus faecalis, methicillin-resistant Staphylococcus aureus (MRSA), Bacillus subtilis spore, Mycobacterium tuberculosis and human immuno-deficiency virus type I. Exposure to Peracetic acid (0.26% w/v) for 10 minutes resulted in massive killing of all the aforementioned organisms and spore.

  6. Nonlinear Dynamic Response of Composite Rotor Blades.

    DTIC Science & Technology

    1986-01-01

    delamination is as follows 2 _2 2LT 1 2 -DN DS Iuj.-,-. °. -,, . , - W. ~fur X NS W_. WU r.. W2 FJUIWXU.~WXV V W V ’ W -. 1 XW F _- Vrr. . wv I ~ 23...the critica fn Ne middle of the laminae as the load is increased. Additional matrix and fiber failure accompany delamination as shown in Table 13. The...J 7.1 %.-6 1. xj k :. v -_- . V~ 7 1- 1’ V. i it- A v LU wV .LJ r~* 00 LJ I-A __ _ _ _ _ _ _ __ _ _ _ _ _ i % 22 A vL . % , lb kA 4 N, (A x LL%. (A

  7. Removal of Volatile Organics from Humidified Air Streams by Absorption.

    DTIC Science & Technology

    1987-12-01

    Activated Carbons : SK-4 nut shell LCL coconut shell WV-B coal base CT coconut shell Specialty Carbons : Graphpac graphitized carbon area...to determine the effects of contaminant concentration, humidity, and chemical competition on the sorption performance of activated carbons . Based on...40 E. ADSORPTION CAPACITY OF ACTIVATED CARBON ............... 40 F. PROCESS ECONOMICS ............................... 45 V

  8. West Virginia Safe and Supportive (S3) Schools Project: Year 2 Implementation Evaluation Report

    ERIC Educational Resources Information Center

    Whisman, Andy

    2013-01-01

    The 4-year federal Safe and Supportive Schools (S3) program supports targeted interventions to improve and measure conditions for learning at the high school level. For 2011-2012 (Year 2), two evaluation questions were investigated: (EQ1) To what extent do participating schools implement the program with fidelity relative to the WV Model for…

  9. Scaling Theory Applied to Far Infrared Absorption by Metal-Insulator Composite Materials

    DTIC Science & Technology

    1989-01-01

    Taylor and Francis, London, 1985), and references therein. 23. H1. J. Hlerrmann, B3. Derrida . and J. Vannimenus, Phys. Rev. B30, 41080 (1984). 24. B... Derrida . D. Stauffer, 11. J. Hlerrmann, and J. Vannimenus, J. Phys. (Paris) 44, L701 (1983). 25. D. WV. lleermann and D. Stauffer, Z. Phys. B44, 339 (1981

  10. 77 FR 60085 - Approval and Promulgation of Air Quality Implementation Plans; West Virginia; The 2002 Base Year...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-02

    ... submitted to meet nonattainment requirements related to West Virginia's portion of the Huntington Area for... 2002 Base Year Inventory for the Huntington Area AGENCY: Environmental Protection Agency (EPA). ACTION... 28, 2009 for the Huntington- Ashland, WV-KY-OH nonattainment area (hereafter referred to as...

  11. 5. Photocopy of photograph, c. 1904. VIEW OF 12,000 VOLT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Photocopy of photograph, c. 1904. VIEW OF 12,000 VOLT TRANSMISSION LINE BETWEEN THE ORIGINAL POWER HOUSE AT DAM NO. 5 AND MARTINSBURG. (From the collection of Mr. Charles Morrison, Hagerstown, MD) - Dam No. 5 Hydroelectric Plant, On Potomac River, Hedgesville, Berkeley County, WV

  12. 42. Photocopy of photograph, c. 1930. DETAIL OF 33,000 VOLT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    42. Photocopy of photograph, c. 1930. DETAIL OF 33,000 VOLT HIGH TENSION SUBSTATION AND STEP UP TRANSFORMERS. (From the collection of the files of Mr. Tony Robucci, Supervisor for Minor Stations, Potomac Edison Company, Hagerstown, MD) - Dam No. 5 Hydroelectric Plant, On Potomac River, Hedgesville, Berkeley County, WV

  13. 6. Credit PEM. Interior of Martinsburg Plant, showing installation of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Credit PEM. Interior of Martinsburg Plant, showing installation of Warren 500 KW, 2200 Volt revolving field a.c. generator and sheave wheel, to be connected to Atlas Corliss 1000 hp steam engine (which doesn't appear in this photo). Photo c. 1907 - Dam No. 4 Hydroelectric Plant, Potomac River, Martinsburg, Berkeley County, WV

  14. 7. Credit PEM. Interior of Martinsburg Plant, showing Warren 500 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Credit PEM. Interior of Martinsburg Plant, showing Warren 500 KW, 2200 Volt generator, with horizontal rode driven sheave wheel to right, belt-driven exciter in center, and switchboard on left. Photo c. 1907. - Dam No. 4 Hydroelectric Plant, Potomac River, Martinsburg, Berkeley County, WV

  15. 75 FR 14491 - Listing of Color Additives Exempt From Certification; Bismuth Citrate

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-26

    ... level of bismuth citrate as a color additive in cosmetics intended for coloring hair on the scalp. This... citrate as a color additive in cosmetics intended for coloring hair on the scalp from 0.5 percent (weight... bismuth citrate in cosmetics intended for coloring scalp hair to 2.0 percent (w/v) with no changes to...

  16. 9. PANORAMIC VIEW WEST, FERNOW EXPERIMENTAL FOREST RESIDENCE, CHEAT DISTRICT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. PANORAMIC VIEW WEST, FERNOW EXPERIMENTAL FOREST RESIDENCE, CHEAT DISTRICT RANGER RESIDENCE AND GARAGE, IMPLEMENT BUILDING, SEED EXTRACTOR BUILDING, CONE DRYING SHED, PUMP HOUSE, OIL HOUSE, CHEAT DISTRICT RANGER OFFICE, WASH HOUSE, AND NURSERY MANAGER'S RESIDENCE. PLANTING BEDS IN BACKGROUND. - Parsons Nursery, South side of U.S. Route 219, Parsons, Tucker County, WV

  17. Fermentative production of butanol from sorghum molasses as a potential agricultural fuel. Final report, June 26, 1981-September 25, 1982

    SciTech Connect

    Fan, L.T.

    1982-12-01

    A strain, Clostridium acetobutylicum ATCC 4259, suitable for butanol-acetone fermentation of sorghum molasses was selected from several strains of the American Type Culture Collection (ATCC). It was cultivated in the composition-optimized sorghum molasses medium. The microbial growth and sugar consumption pattern in the sorghum molasses medium exhibited a typical diauxie phenomenon. The results strongly suggest that the difficulty encountered by the Weizmann type of organisms in butanol-acetone fermentation of molasses is due to the diauxie phenomenon causing a significant decrease in the solvent production rate. Acid hydrolysis of sorghum molasses minimizes the occurrence of the phenomenon, thereby remarkably increasing the solvent yield. The final solvent concentrations in the inverted molasses medium were butanol, 1.0% (w/v); acetone, 0.37% (w/v); ethanol, 0.18% (w/v); and total solvent, 1.55% (w/v). The total solvent yield in the inverted sorghum molasses medium was 30.3% based on the weight of sugar consumed. Effects of the temperature, agitation and heat-shocking were also investigated.

  18. Pollen Biology of Ornamental Ginger (Hedychium spp. J. Koenig)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An improved in vitro pollen germination assay was developed to assess the viability of stored Hedychium pollen. The effect of polyethylene glycol (PEG) (10, 15, and 20% w/v) on pollen germination and tube growth was evaluated for H. longicornutum and two commercial Hedychium cultivars, ‘Orange Brush...

  19. Town Stems Major Water Losses With New Lines and Storage Tank

    EPA Pesticide Factsheets

    With the help of EPA funding, the Town of Chapmanville in Logan County, WV, has a new drinking water storage tank and distribution lines to replace a system built in the late 1940s that was “leaking like a sieve” and posed a risk to public health.

  20. 28 CFR 16.34 - Procedure to obtain change, correction or updating of identification records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Production of FBI Identification Records in Response to... any entry on his/her record to the FBI, Criminal Justice Information Services (CJIS) Division, ATTN: SCU, Mod. D-2, 1000 Custer Hollow Road, Clarksburg, WV 26306. The FBI will then forward the...

  1. 46. Photocopy of photograph, c. 1933. VIEW OF DAM AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    46. Photocopy of photograph, c. 1933. VIEW OF DAM AND FOREBAY. NOTE ALL WATER FLOWING THROUGH FOREBAY AND OUT EITHER TAILRACE OR SLUICE GATE (INSTEAD OF OVER DAM) BECAUSE OF LOW WATER FLOW. (Courtesy of the Potomac Edison Company Library (Hagerstown, MD), Historical Data Files, Dam No. 5 listing - Dam No. 5 Hydroelectric Plant, On Potomac River, Hedgesville, Berkeley County, WV

  2. 43. Credit TR. Reconstruction of Dam No. 4 after 1936 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    43. Credit TR. Reconstruction of Dam No. 4 after 1936 flood. Pouring concrete for new dam section; opening at left for flume to remove water from behind coffer dam. Photo c. 1936 - Dam No. 4 Hydroelectric Plant, Potomac River, Martinsburg, Berkeley County, WV

  3. 58. Photocopy of photograph, no date. VIEW OF GENERATOR UNIT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    58. Photocopy of photograph, no date. VIEW OF GENERATOR UNIT 2. (Courtesy of the Potomac Edison Company Library (Hagerstown, MD), Historical Data Files, Properties-Buildings and Equipment listing) - Dam No. 5 Hydroelectric Plant, On Potomac River, Hedgesville, Berkeley County, WV

  4. Corn steep liquor as a nutrition adjunct for the production of Aspergillus niger lipase and hydrolysis of oils thereof.

    PubMed

    Edwinoliver, N G; Thirunavukarasu, K; Purushothaman, S; Rose, C; Gowthaman, M K; Kamini, N R

    2009-11-25

    Corn steep liquor (CSL) has been used as a nutrition adjunct for the production of an extracellular lipase from Aspergillus niger, which has immense importance as an additive in laundry detergent formulations. A five-level four-factorial central composite design was chosen to determine the optimal medium components with four critical variables, namely, CSL, NH4H2PO4, Na2HPO4, and sesame oil, that were found to be influential for lipase production by the classical one-factor-at-a-time method. The model suggested that all of the factors chosen had a significant impact on lipase production, and the optimum values of the influential parameters were CSL, 2.0%, w/v; NH4H2PO4, 0.05%, w/v; Na2HPO4, 0.75%, w/v; and sesame oil, 2.0%, w/v, with an activity of 26.7 U/mL at 48 h and 30 degrees C, which was 2.16-fold higher than the initial activity (12 U/mL) obtained by the conventional one-factor-at-a-time method. Furthermore, the enzyme has good potential for the hydrolysis of vegetable oils and fish oils, and a hydrolytic ratio of 88.73% was obtained with palm oil at 48 h. The utilization of CSL and sesame oil for lipase production from A. niger makes the process green, because both are renewable substrates and economically viable at an industrial scale.

  5. Accuracy of activPAL Self-Attachment Methods

    ERIC Educational Resources Information Center

    Kringen, Nina L.; Healy, Genevieve N.; Winkler, Elisabeth A. H.; Clark, Bronwyn K.

    2016-01-01

    This study examined the accuracy of self-attachment of the activPAL activity monitor. A convenience sample of 50 participants self-attached the monitor after being presented with written material only (WMO) and then written and video (WV) instructions; and completed a questionnaire regarding the acceptability of the instructional methods.…

  6. 34. Credit JTL. Second floor, view of Monarch Co. Corn ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    34. Credit JTL. Second floor, view of Monarch Co. Corn cracking machine, by Sprout, Waldron and Co., (Muncy, PA), which cut and diced corn to a uniform size, then separated it into three grades of cracked corn and corn meal and removed chaff. - Bunker Hill Mill, County Route 26, Bunker Hill, Berkeley County, WV

  7. 77 FR 76883 - Approval and Promulgation of Implementation Plans and Designation of Areas for Air Quality...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ... Ohio's request to redesignate the Ohio portion of the Huntington- Ashland (OH-WV-KY) nonattainment area... Huntington-Ashland area has attained the 1997 annual PM 2.5 standard, and proposed to approve Ohio's request to redesignate the Ohio portion of the area on December 22, 2011. EPA's final rulemaking...

  8. 16. MAN LADLING GLASS FROM POT. STEAM IS CAUSED BY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. MAN LADLING GLASS FROM POT. STEAM IS CAUSED BY WATER COOLING MOLTEN GLASS IN POT. NOTE GLASS THAT HAS POURED OVER THE SIDE OF THE POT. - Seneca Glass Company Factory, Beechurst Avenue between Sixth & Eighth Streets, Morgantown, Monongalia County, WV

  9. Effect of heat treatment and irradiation temperature on mechanical properties and structure of reduced-activation Cr-W-V steels of bainitic, martensitic, and martensitic-ferritic classes

    NASA Astrophysics Data System (ADS)

    Gorynin, I. V.; Rybin, V. V.; Kursevich, I. P.; Lapin, A. N.; Nesterova, E. V.; Klepikov, E. Yu

    2000-12-01

    Effects of molybdenum replacement by tungsten in steels of the bainitic, martensitic, and martensitic-ferritic classes containing 2.5%, 8% and 11% Cr, respectively, were investigated. The phase composition and structure of the bainitic steels were varied by changing the cooling rates from the austenitization temperature (from values typical for normalization up to V=3.3 × 10-2°C/s) and then tempering. The steels were irradiated to a fluence of 4×1023 n/m2 (⩾0.5 MeV) at 270°C and to fluences of 1.3×1023 and 1.2×1024 n/m2 (⩾0.5 MeV) at 70°C. The 2.5Cr-1.4WV and 8Cr-1.5WV steels have shown lower values of the shifts in ductile-brittle transition temperature (DBTT) under irradiation in comparison with corresponding Cr-Mo steels. Radiation embrittlement at elevated irradiation temperature was lowest in bainitic 2.5Cr-1.4WV steel and martensitic-ferritic 11Cr-1.5WV steel. The positive effect of molybdenum replacement by tungsten at irradiation temperature ∼300°C is reversed at Tirr=70∘C.

  10. Geospatial mapping of vegetation in the Antarctic environment using very high-resolution WorldView-2 imagery

    NASA Astrophysics Data System (ADS)

    Jawak, Shridhar D.; Udhayaraj, A. D.; Alvarinho, Luis J.

    2016-05-01

    A robust monitoring of the changes in the distribution and density of cryospheric plant species requires accurate and high-resolution baseline maps of vegetation. Mapping such change at the landscape scale is often problematic, particularly in remote areas, such as Antarctica. Vegetation mapping of plant communities at fine spatial scales is increasingly supported by remote sensing technology in cryospheric regions. Less frequent imaging with high spatial resolution satellite sensors enable more detailed analyses of vegetation change frequently. This study is the first to use high-resolution WorldView-2 (WV-2) imagery to classify vegetation communities on Antarctic oases and to provide semi-automated means to map vegetation, as an imperative indicator for environmental change. Multispectral imagery (MSI) and panchromatic imagery (PAN) from very high resolution WV-2 have been used for mapping of vegetation in different forms in Antarctic environment. A range of supervised classification methods have been executed using pan-sharpened WV-2 data. This study comparatively and statistically evaluates vegetation mapping results using supervised and unsupervised classification methods to extract vegetation in Larsemann Hills and Schirmacher oasis, east Antarctica. We also discuss on the use of supervised pixel-based classifiers and textural measures, in addition to standard multispectral information, to improve the classification of Antarctic vegetation communities. Classification results were validated with independent reference datasets. This work indicates that the overall accuracy of mapping vegetation using WV-2 imagery and semi-automated target extraction methods ranged from 90% to 94%.

  11. Evaluation of a Novel Rapid Test System for the Detection of Specific IgE to Hymenoptera Venoms

    PubMed Central

    Pfender, Nikolai; Lucassen, Ralf; Offermann, Nadine; Schulte-Pelkum, Johannes; Fooke, Margrit; Jakob, Thilo

    2012-01-01

    Background. The Allergy Lateral Flow Assay (ALFA) is a novel rapid assay for the detection of sIgE to allergens. The objective of this study is the evaluation of ALFA for the detection of sIgE to bee venom (BV) and wasp venom (WV) in insect venom allergic patients. Methods. Specific IgE to BV and WV was analyzed by ALFA, ALLERG-O-LIQ, and ImmunoCAP in 80 insect venom allergic patients and 60 control sera. Sensitivity and specificity of ALFA and correlation of ALFA and ImmunoCAP results were calculated. Results. The sensitivity/specificity of ALFA to the diagnosis was 100%/83% for BV and 82%/97% for WV. For insect venom allergic patients, the Spearman correlation coefficient for ALFA versus ImmunoCAP was 0.79 for BV and 0.80 for WV. However, significant differences in the negative control groups were observed. Conclusion. ALFA represents a simple, robust, and reliable tool for the rapid detection of sIgE to insect venoms. PMID:22500188

  12. Protective actions of green tea polyphenols and alfacalcidol on bone microstructure in female rats with chronic inflammation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study investigated the effects of green tea polyphenols (GTP) and alfacalcidol on bone microstructure and strength along with possible mechanisms in rats with chronic inflammation. A 12-week study using a 2 (no GTP vs. 0.5%, w/v GTP in drinking water) × 2 (no alfacalcidol vs. 0.05 ug/kg alfacal...

  13. 36. INTERIOR VIEW, NORTON GRINDER, TYPES USED TO GRIND ROUGH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    36. INTERIOR VIEW, NORTON GRINDER, TYPE-S USED TO GRIND ROUGH EDGES OFF THE FORGED TOOLS; NOTE OPERATOR IS FINISH GRINDING BLADE END OF A POST HOLE DIGGER AND TAMPING BAR - Warwood Tool Company, Foot of Nineteenth Street, Wheeling, Ohio County, WV

  14. 30. William E. Barrett, Photographer, August 1975. SAWMILL SORTING DOCK ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. William E. Barrett, Photographer, August 1975. SAWMILL SORTING DOCK SHOWING THREE TRACKS OF MOVING CHAIN CONVEYOR. LUMBER WAS UNLOADED ON BOTH SIDES AND PLACED ON CARTS WHOSE RAILS MAY BE SEEN AT LEFT. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  15. 31. William E. Barrett, Photographer, August 1975. SORTING DOCK LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    31. William E. Barrett, Photographer, August 1975. SORTING DOCK LOOKING EAST TOWARD LUMBERYARD. NOTE RAILS FOR LUMBER CARTS. CARTS WERE UNLOADED AT EDGE OF DOCK AND LUMBER WAS PLACED ON FLATCARS TO BE TAKEN TO YARD. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  16. Water vapor permeability, mechanical, optical and sensorial properties of plasticized guar gumedible films

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Edible films were prepared by casting method using guar gum and glycerol in different ratios. The concentration of guar gum was 1.0, 1.5 and 2.0% whereas glycerol concentration was 20, 30 and 40% (w/v). The water vapor permeability (WVP), mechanical properties (tensile strength and elongation), thic...

  17. Synthesis, kinetics and photocatalytic study of "ultra-small" Ag-NPs obtained by a green chemistry method using an extract of Rosa 'Andeli' double delight petals.

    PubMed

    Suárez-Cerda, Javier; Alonso-Nuñez, Gabriel; Espinoza-Gómez, Heriberto; Flores-López, Lucía Z

    2015-11-15

    This paper reports the effect of different concentrations of Rosa 'Andeli' double delight petals aqueous extract (PERA) in the synthesis of silver nanoparticles (Ag-NPs), using an easy green chemistry method. Its kinetics study and photocatalytic activity were also evaluated. The Ag-NPs were obtained using an aqueous silver nitrate solution (AgNO3) with 9.66% w/v, 7.25% w/v, and 4.20% w/v PERA as both reducing-stabilizing agent. The formation of the Ag-NPs was demonstrated by analysis of UV-vis spectroscopy, scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) and transmission electron microscopy (TEM). TEM analysis shows spherical nanoparticles in shape and size between ∼0.5 and 1.4nm. A comparative study was done to determine which concentration was the best reducing-stabilizing agent, and we found out that "ultra-small" nanoparticles (0.5-1.1nm) were obtained with 9.66% w/v of PERA. The size of the Ag-NPs depends on the concentration of PERA and Ag(I). The reaction of formation of "ultra-small" Ag-NPs, proved to be first order for metallic precursor (silver) and second order for reducing-stabilizing agent (PERA). The Ag-NPs showed photocatalytic activity, in degradation of commercial dye with an efficiency of 95%.

  18. Training Workers for Environmental Clean-Up Jobs.

    ERIC Educational Resources Information Center

    Baldwin, Fred

    1993-01-01

    Describes the International Union of Operating Engineers (IUOE) Hazmat Training Program (Beckley, WV). It is the largest program in the nation for training skilled workers to safely handle toxic and hazardous wastes. This program has produced more than 450 certified instructors, who, in turn, have trained more than 18,000 workers. (LP)

  19. Nonthermal inactivation of Escherichia coli K12 in buffered peptone water using a pilot-plant scale supercritical carbon dioxide system with gas-liquid porous metal contractor

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study evaluated the effectiveness of a supercritical carbon dioxide (SCCO2) system, with a gas-liquid CO2 contactor, for reducing Escherichia coli K12 in diluted buffered peptone water. 0.1% (w/v) buffered peptone water inoculated with E. coli K12 was processed using the SCCO2 system at CO2 con...

  20. Inferring Causes of Biological Impairment in the Clear Fork Watershed, West Virginia (Final)

    EPA Science Inventory

    The Clear Fork, WV case study illustrates a causal assessment in a watershed using the USEPA stressor identification process described on www.epa.gov/caddis. This case study demonstrates that a watershed-wide causal assessment has several advantages for making analysis practical,...

  1. Immobilization of Erwinia sp. D12 Cells in Alginate-Gelatin Matrix and Conversion of Sucrose into Isomaltulose Using Response Surface Methodology

    PubMed Central

    Kawaguti, Haroldo Yukio; Carvalho, Priscila Hoffmann; Figueira, Joelise Alencar; Sato, Hélia Harumi

    2011-01-01

    Isomaltulose is a noncariogenic reducing disaccharide and also a structural isomer of sucrose and is used by the food industry as a sucrose replacement. It is obtained through enzymatic conversion of microbial sucrose isomerase. An Erwinia sp. D12 strain is capable of converting sucrose into isomaltulose. The experimental design technique was used to study the influence of immobilization parameters on converting sucrose into isomaltulose in a batch process using shaken Erlenmeyer flasks. We assessed the effect of gelatin and transglutaminase addition on increasing the reticulation of granules of Erwinia sp. D12 cells immobilized in alginate. Independent parameters, sodium alginate concentration, cell mass concentration, CaCl2 concentration, gelatin concentration, and transglutaminase concentration had all a significant effect (P < 0.05) on isomaltulose production. Erwinia sp. D12 cells immobilized in 3.0% (w/v) sodium alginate, 47.0% (w/v) cell mass, 0.3 molL−1 CaCl2, 1.7% (w/v) gelatin and 0.15% (w/v) transglutaminase presented sucrose conversion into isomaltulose, of around 50–60% in seven consecutive batches. PMID:21785708

  2. View southwest, buildings of the Abraham Cyrus Farmstead. (Left to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View southwest, buildings of the Abraham Cyrus Farmstead. (Left to right in photograph: hen house, chicken shed, equipment shed, large tree at center foreground, barn, evergreen in yard, wash house, farmhouse - Abraham Cyrus Farmstead, 3271 Cyrus Road (County Road 1/6), Cyrus, Wayne County, WV

  3. Topical Hazard Evaluation Program of Candidate Insect Repellents AI3-38352a, AI3-38354a, AI3-38355a, US Department of Agriculture Proprietary Chemicals, May 1982 - November 1983.

    DTIC Science & Technology

    1983-11-01

    irrita- chemical and of 10% (wv) A13-38357a, AI3-38360a, tion reaction under Oil of Bergamot (positive and Ar3-38361a did test conditions and control...caused a greater Irritant positive control (Oil of effect than In un- Bergamot ) and diluent were Irradiated skin areas. applied to additional skin

  4. Topical Hazard Evaluation Program of Candidate Insect Repellent A13-38349a US Department of Agriculture Proprietary Chemicals, July 1981-January 1983.

    DTIC Science & Technology

    1983-08-16

    reaction (w/v) Oil of Bergamot irritation reaction under test (positive control) in 95% under test conditions and is ethyl alcohol was conditions, not...the rabbits, 0.05 mL cation and irradiation of the test chemical, caused greater irritant positive control (oil of effects than in un- Bergamot ) and

  5. The impact of particle size and initial solid loading on thermochemical pretreatment of wheat straw for improving sugar recovery.

    PubMed

    Rojas-Rejón, Oscar A; Sánchez, Arturo

    2014-07-01

    This work studies the effect of initial solid load (4-32 %; w/v, DS) and particle size (0.41-50 mm) on monosaccharide yield of wheat straw subjected to dilute H(2)SO(4) (0.75 %, v/v) pretreatment and enzymatic saccharification. Response surface methodology (RSM) based on a full factorial design (FFD) was used for the statistical analysis of pretreatment and enzymatic hydrolysis. The highest xylose yield obtained during pretreatment (ca. 86 %; of theoretical) was achieved at 4 % (w/v, DS) and 25 mm. The solid fraction obtained from the first set of experiments was subjected to enzymatic hydrolysis at constant enzyme dosage (17 FPU/g); statistical analysis revealed that glucose yield was favored with solids pretreated at low initial solid loads and small particle sizes. Dynamic experiments showed that glucose yield did not increase after 48 h of enzymatic hydrolysis. Once established pretreatment conditions, experiments were carried out with several initial solid loading (4-24 %; w/v, DS) and enzyme dosages (5-50 FPU/g). Two straw sizes (0.41 and 50 mm) were used for verification purposes. The highest glucose yield (ca. 55 %; of theoretical) was achieved at 4 % (w/v, DS), 0.41 mm and 50 FPU/g. Statistical analysis of experiments showed that at low enzyme dosage, particle size had a remarkable effect over glucose yield and initial solid load was the main factor for glucose yield.

  6. Immobilization of Erwinia sp. D12 Cells in Alginate-Gelatin Matrix and Conversion of Sucrose into Isomaltulose Using Response Surface Methodology.

    PubMed

    Kawaguti, Haroldo Yukio; Carvalho, Priscila Hoffmann; Figueira, Joelise Alencar; Sato, Hélia Harumi

    2011-01-01

    Isomaltulose is a noncariogenic reducing disaccharide and also a structural isomer of sucrose and is used by the food industry as a sucrose replacement. It is obtained through enzymatic conversion of microbial sucrose isomerase. An Erwinia sp. D12 strain is capable of converting sucrose into isomaltulose. The experimental design technique was used to study the influence of immobilization parameters on converting sucrose into isomaltulose in a batch process using shaken Erlenmeyer flasks. We assessed the effect of gelatin and transglutaminase addition on increasing the reticulation of granules of Erwinia sp. D12 cells immobilized in alginate. Independent parameters, sodium alginate concentration, cell mass concentration, CaCl(2) concentration, gelatin concentration, and transglutaminase concentration had all a significant effect (P < 0.05) on isomaltulose production. Erwinia sp. D12 cells immobilized in 3.0% (w/v) sodium alginate, 47.0% (w/v) cell mass, 0.3 molL(-1) CaCl(2), 1.7% (w/v) gelatin and 0.15% (w/v) transglutaminase presented sucrose conversion into isomaltulose, of around 50-60% in seven consecutive batches.

  7. 1. Photocopy of map (from The Virginia Springs, and the ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Photocopy of map (from The Virginia Springs, and the Springs of the South and West by Moorman) No date 'MAP OF ROUTES AND DISTANCES TO THE VIRGINIA SPRINGS' - White Sulphur Springs, U.S. Route 60, White Sulphur Springs, Greenbrier County, WV

  8. 75 FR 23760 - Notice of Public Hearing Regarding Environmental Protection Agency Region III's Proposed...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-04

    ... Withdrawal of Specification), of an Area as a Disposal Site; Spruce No. 1 Surface Mine, Logan County, WV... Site; Spruce No. 1 Surface Mine, Logan County, West Virginia in the Federal Register soliciting... interest to conduct a public hearing on the Proposed 404(c) Determination for the Spruce No. 1 Surface...

  9. Optimization of freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel.

    PubMed

    Mehrnoush, Amid; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd

    2012-01-01

    Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel. The effect of pectinase content (-2.66, 62.66 mg/mL), Arabic gum (-1.21, 10.21%, w/v), and maltodextrin (0.73, 7.26%, w/v) as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p < 0.05) effect on pectinase activity and yield of the enzyme. It was observed that the interaction effect of Arabic gum and maltodextrin improved the enzymatic properties of freeze-dried pectinase. The optimal conditions for freeze-dried pectinase from mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v) of Arabic gum, and 4 (%, w/v) of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL), yield (86.4%) and storage stability (84.2%) of encapsulated pectinase were achieved.

  10. Effect of Mangifera indica L. extract (QF808) on protein and hepatic microsome peroxidation.

    PubMed

    Martínez, G; Giuliani, A; León, O S; Pérez, G; Núñez Selles, A J

    2001-11-01

    The antioxidant activities of QF808, a steam bark extract of Mangifera indica L., were studied on hydroxyl-mediated oxidation of bovine serum albumin (BSA) and in a hepatic microsome system. The extract was effective in reducing the oxidation of BSA, since its half- maximal inhibition concentration (IC(50)) was 0.0049% w/v in the inhibition of carbonyl group formation and lower than 0.0025% w/v in the inhibition of sulfhydryl group loss. QF808 inhibited lipid peroxidation which was initiated enzymatically by reduced nicotinamide adenine dinucleotide phosphate (NADPH), IC(50)= 0.00075% w/v, or non-enzymatically by ascorbic acid, IC(50) = 0.0126% w/v. The extract tested did not inhibit NADPH-dependent cytochrome P-450 reductase activity, since it had no effect on the oxidation rate of NADPH. These results suggest that QF808 has an antioxidant activity, probably due to its ability to scavenge free radicals involved in microsome lipid peroxidation. In addition, QF808 antioxidant profile in vitro is probably similar to its principal polyphenolic component, mangiferin, a glycosylated xanthone.

  11. Properties of cassava starch-based edible coating containing essential oils.

    PubMed

    Oriani, Vivian Boesso; Molina, Gustavo; Chiumarelli, Marcela; Pastore, Gláucia Maria; Hubinger, Miriam Dupas

    2014-02-01

    Edible coatings were produced using cassava starch (2% and 3% w/v) containing cinnamon bark (0.05% to 0.30% v/v) or fennel (0.05% to 0.30% v/v) essential oils. Edible cassava starch coating at 2% and 3% (w/v) containing or not containing 0.30% (v/v) of each essential oils conferred increased in water vapor resistance and decreased in the respiration rates of coated apple slices when compared with uncoated fruit. Cassava starch coatings (2% w/v) added 0.10% or 0.30% (v/v) fennel or cinnamon bark essential oils showed antioxidant capacity, and the addition of 0.30% (v/v) of each essential oil demonstrated antimicrobial properties. The coating containing cinnamon bark essential oil showed a significant antioxidant capacity, comparing to fennel essential oil. Antimicrobial tests showed that the addition of 0.30% (v/v) cinnamon bark essential oil to the edible coating inhibited the growth of Staphylococcus aureus and Salmonella choleraesuis, and 0.30% fennel essential oil inhibited just S. aureus. Treatment with 2% (w/v) of cassava starch containing 0.30% (v/v) of the cinnamon bark essential oil showed barrier properties, an antioxidant capacity and microbial inhibition.

  12. Caries Experience Differs between Females and Males across Age Groups in Northern Appalachia

    PubMed Central

    Shaffer, John R.; Leslie, Elizabeth J.; Feingold, Eleanor; Govil, Manika; McNeil, Daniel W.; Crout, Richard J.; Weyant, Robert J.; Marazita, Mary L.

    2015-01-01

    Sex disparities in dental caries have been observed across many populations, with females typically exhibiting higher prevalence and more affected teeth. In this study we assessed the sex disparities in two Northern Appalachian populations from West Virginia (WV, N = 1997) and Pennsylvania (PA, N = 1080) by comparing caries indices between males and females across four phases of dental development: primary dentition in children aged 1–5 years, mixed dentition in children aged 6–11 years, permanent dentition in adolescents aged 12–17 years, and permanent dentition in adults aged 18–59 years. No significant sex differences were observed for children aged 1–5 years. Contrary to national and international trends, WV girls aged 6–11 years had 1.5 fewer affected teeth than boys (p < 0.001). However, by ages 12–17, caries indices in the WV girls matched those in boys. In both WV and PA adults, women and men had similar total counts of affected teeth (i.e., DMFT), although women had more dental restorations (p < 0.001) and men had more current decay (p < 0.001). These results suggest that in some Appalachian populations, young girls benefit from protection against caries that is lost during adolescence and that adult women utilize dental health care to a greater degree than men. PMID:26106416

  13. 76 FR 75464 - Approval and Promulgation of Air Quality Implementation Plans; West Virginia and Ohio...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-02

    ... each of these Areas pursuant to 40 CFR 51.1004(c). These determinations were published in the Federal...-Marietta and Wheeling Nonattainment Areas AGENCY: Environmental Protection Agency (EPA). ACTION: Final rule... particle (PM 2.5 ) nonattainment area and the Wheeling, WV-OH PM 2.5 nonattainment ] area...

  14. Biological abatement of cellulase inhibitors

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Bio-abatement uses a fungus to metabolize and remove fermentation inhibitors. To determine whether bio-abatement could alleviate enzyme inhibitor effects observed in biomass liquors after pretreatment, corn stover at 10% (w/v) solids was pretreated with either dilute acid or liquid hot water. The ...

  15. An Evaluation of Naloxone Use for Opioid Overdoses in West Virginia: A Literature Review.

    PubMed

    Beheshti, Annahita; Lucas, Landyn; Dunz, Tanika; Haydash, Maryna; Chiodi, Hope; Edmiston, Breanna; Ford, Chad; Bohn, Natalie; Stein, John Hunter; Berrett, Anthony; Sobota, Brittney; Horzempa, Joseph

    2015-07-09

    Naloxone is a common treatment option for the reversal of an opioid overdose. The law regulating naloxone varies from state to state and therefore so does the drug's availability. The state of Rhode Island has legalized naloxone for over-the-counter use while a number of states allow prescriptions to opioid abusers and family members. The medical community as a whole appears to be divided on the issue of availability to lay persons. This is largely due to a lack of information on this relatively new subject, as well as discrepancies within the existing naloxone research in regard to the use of naloxone among the general public. This literature review provides an objective examination of the pros and cons of increasing the availability of naloxone to the public, with an emphasis on the state of West Virginia (WV). Due to WV's high rates of opioid abuse and overdose, the implications of increasing public accessibility to naloxone could be invaluable in the effort to reduce opioid related deaths. To this end, a WV law that has recently gone into effect allows emergency personnel and family members of opioid addicts to carry naloxone. Ongoing research investigations will determine the impact of this law in regard to the overall wellbeing of the residents of WV and the implications on future laws regulating naloxone use.

  16. 76 FR 69764 - Petitions for Modification of Application of Existing Mandatory Safety Standards

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-09

    ... course refuse as detailed in the Refuse Disposal Plan for refuse site WV04-08438-02. (5) Since the..., West Virginia 25801. Mine: Alpheus Refuse Site, MSHA Mine I.D. No. 46-08438, located in McDowell County, West Virginia. Regulation Affected: 30 CFR 77.214(a) (Refuse piles; general). Modification Request:...

  17. 11. FREIGHT YARDS AT KEYSER. TRAIN SHED AND B & ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. FREIGHT YARDS AT KEYSER. TRAIN SHED AND B & O STORAGE FACILITY ARE VISIBLE AT LEFT. TURNTABLE LIES OUT OF VIEW BEHIND TRAIN SHED. - Baltimore & Ohio Railroad, Keyser Machine Shop, State Route 46 Northwest of Spring Street, Keyser, Mineral County, WV

  18. Effects of temperature, pH and NaCl content on in vitro putrescine and cadaverine production through the growth of Serratia marcescens CCM 303.

    PubMed

    Bubelová, Zuzana; Buňka, František; Taťáková, Monika; Štajnochová, Kateřina; Purevdorj, Khatantuul; Buňková, Leona

    2015-01-01

    The aim of this study was to evaluate the combined effect of temperature (10, 20 and 37°C), pH (4, 5, 6, 7 and 8), and NaCl content (0, 1, 3, 4, 5 and 6% w/v) on the growth and putrescine and cadaverine production of Serratia marcescens CCM 303 under model conditions. The decarboxylase activity of S. marcescens was monitored in broth after cultivation. The cultivation medium was enriched with selected amino acids (ornithine, arginine and lysine; 0.2% w/v each) serving as precursors of biogenic amines. Levels of putrescine and cadaverine in broth were analysed by high-performance liquid chromatography after pre-column derivatisation with o-phthalaldehyde reagent. S. marcescens produced higher amounts of putrescine (up to 2096.8 mg L(-1)) compared to cadaverine content (up to 343.3 mg L(-1)) in all cultivation media. The highest putrescine and cadaverine concentrations were reached during cultivation at 10-20°C, pH 5-7 and NaCl content 1-3% w/v. On the other hand, the highest BAs production of individual cell (recalculated based on a cell; so called "yield factor") was observed at 10°C, pH 4 and salt concentration 3-5% w/v as a response to environmental stress.

  19. Cyclic Hematopoiesis: animal models

    SciTech Connect

    Jones, J.B.; Lange, R.D.

    1983-08-01

    The four existing animal models of cyclic hematopoiesis are briefly described. The unusual erythropoietin (Ep) responses of the W/Wv mouse, the Sl/Sld mouse, and cyclic hematopoietic dog are reviewed. The facts reviewed indicate that the bone marrow itself is capable of influencing regulatory events of hematopoiesis.

  20. Engaging Youth to Examine Lifestyle Behaviors through Authentic Research with University Partnerships

    ERIC Educational Resources Information Center

    Bardwell, Genevieve; Mujuru, Priscah; Fitch, Cindy; Seidel, George; Hu, Wen; Sogodogo, Kalifa; Chester, Ann

    2007-01-01

    University researchers partnered with secondary students in West Virginia and Mali on an international science investigation to strengthen science education and public health practices. WV and Mali students made comparisons of diet, physical activity, BMI, and blood pressure gathered from study participants. Full IRB approval was provided by West…

  1. 3. DETAIL OF INVERTED MINE LOCOMOTIVE OR "MOTOR," USED FOR ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. DETAIL OF INVERTED MINE LOCOMOTIVE OR "MOTOR," USED FOR SURFACE HAULAGE OF MINE CARS, LOOKING SOUTHWEST; NOTE GEARING - Nuttallburg Mine Complex, Main Mine, North side of New River, 2.7 miles upstream from Fayette Landing, Lookout, Fayette County, WV

  2. 18. William E. Barrett, Photographer, August 1975. EXPOSED VIEW OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. William E. Barrett, Photographer, August 1975. EXPOSED VIEW OF LOWER PULLEYS OF LEFT-HAND MILL. LOWER LEFT IS BAND SAW PULLEY. UPPER LEFT IS TENSION WHEEL. LARGE PULLEY ON RIGHT IS DRIVE WHEEL FROM POWER SOURCE. - Meadow River Lumber Company, Highway 60, Rainelle, Greenbrier County, WV

  3. General view of low pressure compressor (unit #3) with compressor ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General view of low pressure compressor (unit #3) with compressor in foreground and engines in background. High pressure stage is on left, low pressure stage is on right. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  4. Detail view of unit 43 with high pressure stage compressor ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail view of unit 43 with high pressure stage compressor in left foreground and low pressure stage compressor in right background. - Burnsville Natural Gas Pumping Station, Saratoga Avenue between Little Kanawha River & C&O Railroad line, Burnsville, Braxton County, WV

  5. 76 FR 75563 - 2002 Reopened-Previously Denied Determinations; Notice of Revised Denied Determinations on...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-02

    ...), Charlestown, WV: May 2, 2010. TA-W-80,166; Computer Sciences Corp (CSC), El Segundo, CA: May 6, 2010. TA-W-80...: March 2, 2010. TA-W-80,028; Affiliated Computer Service, Hillsboro, OR: March 1, 2010....

  6. Intensification of enzymatic hydrolysis of waste newspaper using ultrasound for fermentable sugar production.

    PubMed

    Subhedar, Preeti B; Babu, Narmadha R; Gogate, Parag R

    2015-01-01

    An effective conversion of lignocellulose into fermentable sugars is a key step in producing bioethanol in an eco-friendly and cost effective manner. In this study, the effect of ultrasound on enzymatic hydrolysis of newspaper, a potential feedstock for bioethanol production due to its high cellulosic content, was investigated. The effect of substrate loading, enzyme loading, temperature, ultrasonic power and duty cycle on the hydrolysis has been studied. Optimum conditions for conventional enzymatic hydrolysis were substrate loading of 5% (w/v), enzyme loading of 0.14% (w/v), temperature of 323K, and under these conditions and 72h of hydrolysis, reducing sugar yield of 11.569g/L was obtained. In case of ultrasound-assisted enzymatic hydrolysis approach, optimum conditions obtained were substrate loading of 3% (w/v), enzyme loading of 0.8% (w/v), sonication power of 60W, duty cycle of 70%, hydrolysis time of 6.5h and the reducing sugar yield obtained under these conditions was 27.6g/L. Approximately 2.4 times increase in the release of reducing sugar concentration was obtained by the ultrasound-assisted enzymatic hydrolysis approach. Results indicate that there is a synergistic effect obtained from the combination of ultrasound and enzymes which lowers the diffusion-limiting barrier to enzyme/substrate binding and results in an increase in reaction rate. The experimental data were also fitted in a simple three parameter kinetic model.

  7. 21 CFR 1310.12 - Exempt chemical mixtures.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... concentrations. Iodine 6699 2.2 Calculated as weight/volume (w/v). Isosafrole 8704 20% by Weight or Volume... coatings and film-forming agents. (3) Iodine products classified as iodophors that exist as an iodine complex to include poloxamer-iodine complex, polyvinyl pyrrolidone-iodine complex (i.e.,...

  8. 21 CFR 1310.12 - Exempt chemical mixtures.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... concentrations. Iodine 6699 2.2 Calculated as weight/volume (w/v). Isosafrole 8704 20% by Weight or Volume... coatings and film-forming agents. (3) Iodine products classified as iodophors that exist as an iodine complex to include poloxamer-iodine complex, polyvinyl pyrrolidone-iodine complex (i.e.,...

  9. 21 CFR 1310.12 - Exempt chemical mixtures.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... concentrations. Iodine 6699 2.2 Calculated as weight/volume (w/v). Isosafrole 8704 20% by Weight or Volume... coatings and film-forming agents. (3) Iodine products classified as iodophors that exist as an iodine complex to include poloxamer-iodine complex, polyvinyl pyrrolidone-iodine complex (i.e.,...

  10. 49. INTERIOR VIEW OF HARDENER AREA SHOWING GAUGE THAT MEASURES ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    49. INTERIOR VIEW OF HARDENER AREA SHOWING GAUGE THAT MEASURES HARDNESS, THE NAIL MUST BREAK IN THE CENTER RANGE OF THE CURVED BAR TO HAVE THE CORRECT HARDNESS (THE NAIL WILL BREAK TOO EASILY IF TOO HARD AND WILL BEND TOO MUCH IF TOO SOFT) - LaBelle Iron Works, Thirtieth & Wood Streets, Wheeling, Ohio County, WV

  11. Formulation of Thermosensitive in situ Otic Gel for Topical Management of Otitis Media

    PubMed Central

    Shau, P. A.; Dangre, P. V.; Potnis, V. V.

    2015-01-01

    Antibiotics and antiinflammatory agents are the primary and main therapeutic categories in the treatment of otitis media. One of the simpler and feasible approaches of minimizing the problem of repeated use and subsequent resistance is development of sustained release formulation. Therefore, the present investigation aimed to develop a sustained release in situ gel formulation containing combination of broad spectrum antibiotic and antiinflammatory agents for the management of otits media. The prolonged release is achieved by phase transition of Poloxamer 407 (in situ) from sol to gel at a physiological temperature in combination with viscosity imparting agent Natrasol 250 and Klucel HF. The formulation P3N3 (19% w/v Poloxamer 407, 1.5% w/v Natrasol 250) and P3K3 (19% w/v Poloxamer 407, 1.5% w/v Klucel HF) showed mucoadhesive strength 37.17±0.32×103 and 38.12±0.13×103 dyne/cm2, respectively, and gel strength 2.1 and 2 cm, respectively. Both these formulations indicated good drug content and viscosity besides a good gelling ability. The in vitro diffusion has demonstrated prolongation of release of both the drugs over a period of 8 h. PMID:26997706

  12. 75 FR 38191 - Companies Holding Certificates of Authority as Acceptable Sureties on Federal Bonds and as...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-01

    .... INCORPORATED IN: Delaware. American Automobile Insurance Company (NAIC 21849) BUSINESS ADDRESS: 777 San Marin..., OR, PA, RI, SC, SD, TN, TX, UT, VT, VA, WA, WV, WI, WY. INCORPORATED IN: Florida. ACSTAR INSURANCE... Insurance Company (NAIC 33898) BUSINESS ADDRESS: P.O. Box 3153, Harrisburg, PA 17105. PHONE: (717)...

  13. Horseradish peroxidase-catalyzed formation of hydrogels from chitosan and poly(vinyl alcohol) derivatives both possessing phenolic hydroxyl groups.

    PubMed

    Sakai, Shinji; Khanmohammadi, Mehdi; Khoshfetrat, Ali Baradar; Taya, Masahito

    2014-10-13

    Horseradish peroxidase-catalyzed cross-linking was applied to prepare hydrogels from aqueous solutions containing chitosan and poly(vinyl alcohol) derivatives both possessing phenolic hydroxyl groups (denoted as Ph-chitosan and Ph-PVA, respectively). Comparing the hydrogels prepared from the solution of 1.0% (w/v) Ph-chitosan and 3.0% (w/v) Ph-PVA and that of 3.0% (w/v) Ph-chitosan and 1.0% (w/v) Ph-PVA, the gelation time of the former hydrogel was 47 s, while was 10s longer than that of the latter one. The breaking point for the former hydrogel under stretching (114% strain) was approximately twice larger than that for the latter one. The swelling ratio of the former hydrogel in saline was about half of the latter one. Fibroblastic cells did not adhere on the former hydrogel but adhered and spread on the latter one. The growth of Escherichia coli cells was fully suppressed on the latter hydrogel during 48 h cultivation.

  14. Determination of Selection Criteria for Spray Drift Reduction from Atomization Data

    DTIC Science & Technology

    2012-01-01

    658. [14] Teske , M. E., Thistle, H. W., and Mickle, R. E., “Modeling Finer Droplet Aerial Spray Drift and Deposition,” Trans. ASABE, Vol. 16, 2002, pp...AGDISP, Version 8.24, 2010, U.S. Forest Service, Morgantown, WV. [21] SAS 9.2 for Windows, 2008, SAS Institute Inc., Cary, NC. [22] Teske , M. E

  15. 47 CFR 78.19 - Interference.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...) Radio Astronomy and Radio Research Installations. In order to minimize harmful interference at the National Radio Astronomy Observatory site located at Green Bank, Pocahontas County, W. Va., and at the... Astronomy Observatory, Post Office Box No. 2, Green Bank, WV 24944, in writing, of the technical...

  16. 47 CFR 78.19 - Interference.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) Radio Astronomy and Radio Research Installations. In order to minimize harmful interference at the National Radio Astronomy Observatory site located at Green Bank, Pocahontas County, W. Va., and at the... Astronomy Observatory, Post Office Box No. 2, Green Bank, WV 24944, in writing, of the technical...

  17. 47 CFR 78.19 - Interference.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...) Radio Astronomy and Radio Research Installations. In order to minimize harmful interference at the National Radio Astronomy Observatory site located at Green Bank, Pocahontas County, W. Va., and at the... Astronomy Observatory, Post Office Box No. 2, Green Bank, WV 24944, in writing, of the technical...

  18. 47 CFR 78.19 - Interference.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) Radio Astronomy and Radio Research Installations. In order to minimize harmful interference at the National Radio Astronomy Observatory site located at Green Bank, Pocahontas County, W. Va., and at the... Astronomy Observatory, Post Office Box No. 2, Green Bank, WV 24944, in writing, of the technical...

  19. 47 CFR 78.19 - Interference.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) Radio Astronomy and Radio Research Installations. In order to minimize harmful interference at the National Radio Astronomy Observatory site located at Green Bank, Pocahontas County, W. Va., and at the... Astronomy Observatory, Post Office Box No. 2, Green Bank, WV 24944, in writing, of the technical...

  20. Optimization of Freeze Drying Conditions for Purified Pectinase from Mango (Mangifera indica cv. Chokanan) Peel

    PubMed Central

    Mehrnoush, Amid; Mustafa, Shuhaimi; Yazid, Abdul Manap Mohd

    2012-01-01

    Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel. The effect of pectinase content (−2.66, 62.66 mg/mL), Arabic gum (−1.21, 10.21%, w/v), and maltodextrin (0.73, 7.26%, w/v) as independent variables on activity, yield, and storage stability of freeze-dried enzyme was evaluated. Storage stability of pectinase was investigated after one week at 4 °C and yield percentage of the enzyme after encapsulation was also determined. The independent variables had the most significant (p < 0.05) effect on pectinase activity and yield of the enzyme. It was observed that the interaction effect of Arabic gum and maltodextrin improved the enzymatic properties of freeze-dried pectinase. The optimal conditions for freeze-dried pectinase from mango peel were obtained using 30 mg/mL of pectinase content, 4.5 (%, w/v) of Arabic gum, and 4 (%, w/v) of maltodextrin. Under these conditions, the maximum activity (11.12 U/mL), yield (86.4%) and storage stability (84.2%) of encapsulated pectinase were achieved. PMID:22489134

  1. Schramm-Loewner evolution theory of the asymptotic behaviors of (2 + 1) -dimensional Wolf-Villain model

    NASA Astrophysics Data System (ADS)

    Chen, Yili; Tang, Gang; Xun, Zhipeng; Zhu, Lei; Zhang, Zhe

    2017-01-01

    Although extensive analytical and numerical work has focus on investigating the (2+1)-dimensional Wolf-Villain (WV) model, some problems concerning its asymptotical behaviors, such as the universality class to which it belongs remain controversial. The Schramm-Loewner evolution (SLE) theory is an attractive approach to describe the fluctuation phenomena and random processes, and has also been applied to the analysis of the stochastic growth of surfaces. In this work, we applied SLE theory to the analysis of the saturated surface to conduct in-depth research, with a new perspective, on the asymptotical behaviors of the (2+1)-dimensional WV model. On the basis of the analysis of the saturated surface contour lines, we determine that the diffusion coefficient calculated for (2+1)-dimensional WV model is κ = 2.91 ± 0.01, and that for Family model is κ = 2.88 ± 0.01. Accordingly we can conclude from the view of SLE theory, that the (2+1)-dimensional WV model, similar to the Family model, also belongs to the Edwards-Wilkinson universality class.

  2. Application of multistage continuous fermentation for production of fuel alcohol by very-high-gravity fermentation technology.

    PubMed

    Bayrock, D P; Michael Ingledew, W

    2001-08-01

    A fermentation system to test the merging of very-high-gravity (VHG) and multistage continuous culture fermentation (MCCF) technologies was constructed and evaluated for fuel ethanol production. Simulated mashes ranging from 15% to 32% w/v glucose were fermented by Saccharomyces cerevisiae and the dilution rates were adjusted for each glucose concentration to provide an effluent containing less than 0.3% w/v glucose (greater than 99% consumption of glucose). The MCCF can be operated with glucose concentrations up to 32% w/v, which indicates that the system can successfully operate under VHG conditions. With 32% w/v glucose in the medium reservoir, a maximum of 16.73% v/v ethanol was produced in the MCCF. The introduction of VHG fermentation into continuous culture technology allows an improvement in ethanol productivity while producing ethanol continuously. In comparing the viability of yeast by methylene blue and plate count procedures, the results in this work indicate that the methylene blue procedure may overestimate the proportion of dead cells in the population. Ethanol productivity (Yps) increased from the first to the last fermentor in the sequence at all glucose concentrations used. This indicated that ethanol is more effectively produced in later fermentors in the MCCF, and that the notion of a constant Yps is not a valid assumption for use in mathematical modeling of MCCFs.

  3. Effect of gamma radiation on the mechanical and barrier properties of HEMA grafted chitosan-based films

    NASA Astrophysics Data System (ADS)

    Khan, Avik; Huq, Tanzina; Khan, Ruhul A.; Dussault, Dominic; Salmieri, Stephane; Lacroix, Monique

    2012-08-01

    Chitosan films were prepared by dissolving 1% (w/v) chitosan powder in 2% (w/v) aqueous acetic acid solution. Chitosan films were prepared by solution casting. The values of puncture strength (PS), viscoelasticity coefficient and water vapor permeability (WVP) of the films were found to be 565 N/mm, 35%, and 3.30 g mm/m2 day kPa, respectively. Chitosan solution was exposed to gamma irradiation (0.1-5 kGy) and it was revealed that PS values were reduced significantly (p≤0.05) after 1 kGy dose and it was not possible to form films after 5 kGy. Monomer, 2-hydroxyethyl methacrylate (HEMA) solution (0.1-1%, w/v) was incorporated into the chitosan solution and the formulation was exposed to gamma irradiation (0.3 kGy). A 0.1% (w/v) HEMA concentration at 0.3 kGy dose was found optimal-based on PS values for chitosan grafting. Then radiation dose (0.1-5 kGy) was optimized for HEMA grafting. The highest PS values (672 N/mm) were found at 0.7 kGy. The WVP of the grafted films improved significantly (p≤0.05) with the rise of radiation dose.

  4. Ethanol and co-product generation from pressurized batch hot water pretreated T85 bermudagrass and Merkeron napiergrass using recombinant Escherichia coli as biocatalyst

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pretreatment of grasses is required to maximize ethanol yield during fermentation. T85 bermudagrass and Merkeron napiergrass (Pennisetum purpureum Schumach.) were either left untreated or were pressurized batch hot water (PBHW) pretreated for 2 minutes at 230°C at 5% w/v whole grass solids loading. ...

  5. Implementation of the Master Plan for Statewide Professional Staff Development for 2011-2012: An Evaluation Study

    ERIC Educational Resources Information Center

    Hammer, Patricia Cahape

    2012-01-01

    This evaluation study examined the formation and implementation of the West Virginia Board of Education's Master Plan for Professional Staff Development for 2011-2012 (PD Master Plan). The performance of PD providers included in the PD Master Plan: Marshall University, all eight regional education service agencies (RESAs), the WV Center for…

  6. Condensed-Phase Processes during Solid Propellant Combustion. 3. Preliminary Depth-Profiling Studies on XM39, JA2, M9, M30, and HMX2

    DTIC Science & Technology

    1994-01-01

    Campus Library W.C. Strahle ATTN: K. Brezinsky B.T. Zinn I. Glassman Atlanta, GA 30332 P.O. Box 710 Princeton, NJ 08540 University of Illinois Dept. of...White Rocket Center, WV 26726 B i3. Wenzel 345 E. 47th Street 1 Hercules, Inc. New York, NY 10017 ATTN: R.V. Cartwright 100 Howard Blvd. Atlantic

  7. A Preliminary Development of a Nuclear Propulsion Officer Enlistment Supply Model.

    DTIC Science & Technology

    1986-09-01

    candidate has withi the Navy and therctlOre are the source of’ initial impresions and fict al information regarding the Nuclear Navy. I his...Mulch 3D ~epartment of Qperations Research Naval Posteraduate School MIonterey, California 93943 5. Professor George WV. T-homas 3 Naval Postcraduate

  8. 40 CFR 62.12150 - Identification of plan.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 9 2012-07-01 2012-07-01 false Identification of plan. 62.12150... amended on April 19, 2000. (b) On May 11, 2009, the West Virginia Department of Environmental Protection... regulatory requirements into one modified rule, WV45CSR18. (c) On September 7, 2011 the West...

  9. Some Dimensions of Intercultural Variation and Their Implication for Interpersonal Behavior

    DTIC Science & Technology

    1981-05-01

    with loneliness and rejection. People do not obtain privacy in a physical sense, though they undoubtedly have private thoughts and daydreams while in...H. Russell Bernard FPO New York 09510 Dept. of Sociology & Anthropology West Virginia University Commander in Chief Morgantown, WV 26506 Human

  10. Developing a strawberry yogurt fortified with marine fish oil

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fortified dairy products appeal to a wide variety of consumers and have the potential to increase sales in the yogurt industry and contribute to boost the intake of omega-3 fatty acids. The objectives of this study were to develop a strawberry yogurt containing microencapsulated salmon oil (2% w/v) ...

  11. The use of xanthan gum in an ophthalmic liquid dosage form: rheological characterization of the interaction with mucin.

    PubMed

    Ceulemans, Jens; Vinckier, Inge; Ludwig, Annick

    2002-04-01

    The development of an ocular dosage form containing xanthan gum and capable of interacting with mucin in the precorneal area is a challenge. The polymer concentration that can be applied is restricted because of the limited patient acceptability of highly viscous preparations. The precorneal mucin concentration is low and the high ionic strength of the lachrymal fluid forces xanthan gum in an ordered structure, less capable of interacting through heterotypic junctions. Intrinsic viscosity measurements and shear rheometry are used to investigate the effect of several factors (polymer concentration, additional boiling or sonication step to the preparation procedure) on the physicochemical properties of xanthan gum and the degree of interaction with a low (8%, w/v) and high (16.0%, w/v) concentrated mucin dispersion. Independent of the preparation procedure applied, a xanthan gum concentration of 1.0% (w/v) is required to obtain a measurable interaction with mucin. If an extra boiling or sonication step is added to the standard preparation procedure, the minimum mucin concentration necessary to achieve formation of heterotypic junctions is decreased. Only by sonication of the highly concentrated xanthan gum dispersion is the viscosity decreased to a level that is tolerable and comfortable to the patient. The findings of the present study clearly demonstrate that a significant interaction between a tolerable and comfortable ocular dosage form containing xanthan gum, and mucin 8% (w/v), is feasible after sonication of a highly concentrated polymer dispersion.

  12. 21. INTERIOR VIEW, UNDER THE MAIN FLOOR SHOWING THE LINESHAFT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. INTERIOR VIEW, UNDER THE MAIN FLOOR SHOWING THE LINESHAFT SYSTEM ONCE POWERED BY A STEAM ENGINE AND LATER BY TWO LARGE ELECTRICAL MILL MOTORS (NOTICE LARGE GEAR IN FOREGROUND) THAT OPERATED EACH NAIL MACHINE; PRESENTLY THE NAIL MACHINES ARE DRIVEN BY INDIVIDUAL ELECTRICAL MOTORS - LaBelle Iron Works, Thirtieth & Wood Streets, Wheeling, Ohio County, WV

  13. 15. INTERIOR OF POWER PLANT LOOKING SOUTHWEST. BACK SIDE OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. INTERIOR OF POWER PLANT LOOKING SOUTHWEST. BACK SIDE OF ELECTRICAL PANEL ON LEFT, AND C. 1910 GENERATOR COVER ON RIGHT. - Potomac Power Plant, On West Virginia Shore of Potomac River, about 1 mile upriver from confluence with Shenandoah River, Harpers Ferry, Jefferson County, WV

  14. DETAIL OF CONNECTION TO SWITCH POINTS OF TURNOUT 29, LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL OF CONNECTION TO SWITCH POINTS OF TURNOUT 29, LOOKING NORTHWEST. SILVER BOX HOUSES ELECTRICAL CONTACTS FOR POSITION FEEDBACK TO SIGNAL CIRCUITS. SHEET METAL COVERS GAS-FIRED HEATER FOR FREEZE PROTECTION IN WINTER. - Baltimore & Ohio Railroad, Z Tower, State Route 46, Keyser, Mineral County, WV

  15. DETAIL OF CONNECTION TO SWITCH POINTS OF TURNOUT 29, LOOKING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL OF CONNECTION TO SWITCH POINTS OF TURNOUT 29, LOOKING EAST. SILVER BOX HOUSES ELECTRICAL CONTACTS FOR POSITION FEEDBACK TO SIGNAL CIRCUITS. SHEET METAL COVERS GAS-FIRED SWITCH HEATER FOR FREEZE PROTECTION IN WINTER. - Baltimore & Ohio Railroad, Z Tower, State Route 46, Keyser, Mineral County, WV

  16. The 4-H Club Meeting: An Essential Youth Development Strategy

    ERIC Educational Resources Information Center

    Cassels, Alicia; Post, Liz; Nestor, Patrick I.

    2015-01-01

    The club meeting has served as a key delivery method for 4-H programming across the United States throughout its history. A survey of WV 4-H community club members reinforces the body of evidence that the 4-H club meeting is an effective vehicle for delivering positive youth learning opportunities within the umbrella of the Essential Elements of…

  17. 28 CFR 16.34 - Procedure to obtain change, correction or updating of identification records.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Production of FBI Identification Records in Response to... any entry on his/her record to the FBI, Criminal Justice Information Services (CJIS) Division, ATTN: SCU, Mod. D-2, 1000 Custer Hollow Road, Clarksburg, WV 26306. The FBI will then forward the...

  18. 28 CFR 16.34 - Procedure to obtain change, correction or updating of identification records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Production of FBI Identification Records in Response to... any entry on his/her record to the FBI, Criminal Justice Information Services (CJIS) Division, ATTN: SCU, Mod. D-2, 1000 Custer Hollow Road, Clarksburg, WV 26306. The FBI will then forward the...

  19. 28 CFR 16.34 - Procedure to obtain change, correction or updating of identification records.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Production of FBI Identification Records in Response to... any entry on his/her record to the FBI, Criminal Justice Information Services (CJIS) Division, ATTN: SCU, Mod. D-2, 1000 Custer Hollow Road, Clarksburg, WV 26306. The FBI will then forward the...

  20. 28 CFR 16.34 - Procedure to obtain change, correction or updating of identification records.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... PRODUCTION OR DISCLOSURE OF MATERIAL OR INFORMATION Production of FBI Identification Records in Response to... any entry on his/her record to the FBI, Criminal Justice Information Services (CJIS) Division, ATTN: SCU, Mod. D-2, 1000 Custer Hollow Road, Clarksburg, WV 26306. The FBI will then forward the...