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Sample records for abiotic control experiments

  1. Interactive biotic and abiotic regulators of soil carbon cycling: evidence from controlled climate experiments on peatland and boreal soils.

    PubMed

    Briones, María Jesús I; McNamara, Niall P; Poskitt, Jan; Crow, Susan E; Ostle, Nicholas J

    2014-09-01

    Partially decomposed plant and animal remains have been accumulating in organic soils (i.e. >40% C content) for millennia, making them the largest terrestrial carbon store. There is growing concern that, in a warming world, soil biotic processing will accelerate and release greenhouse gases that further exacerbate climate change. However, the magnitude of this response remains uncertain as the constraints are abiotic, biotic and interactive. Here, we examined the influence of resource quality and biological activity on the temperature sensitivity of soil respiration under different soil moisture regimes. Organic soils were sampled from 13 boreal and peatland ecosystems located in the United Kingdom, Ireland, Spain, Finland and Sweden, representing a natural resource quality range of C, N and P. They were incubated at four temperatures (4, 10, 15 and 20 °C) at either 60% or 100% water holding capacity (WHC). Our results showed that chemical and biological properties play an important role in determining soil respiration responses to temperature and moisture changes. High soil C : P and C : N ratios were symptomatic of slow C turnover and long-term C accumulation. In boreal soils, low bacterial to fungal ratios were related to greater temperature sensitivity of respiration, which was amplified in drier conditions. This contrasted with peatland soils which were dominated by bacterial communities and enchytraeid grazing, resulting in a more rapid C turnover under warmer and wetter conditions. The unexpected acceleration of C mineralization under high moisture contents was possibly linked to the primarily role of fermented organic matter, instead of oxygen, in mediating microbial decomposition. We conclude that to improve C model simulations of soil respiration, a better resolution of the interactions occurring between climate, resource quality and the decomposer community will be required.

  2. Experiments on the abiotic amplification of optical activity

    NASA Technical Reports Server (NTRS)

    Bonner, W. A.; Blair, N. E.; Dirbas, F. M.

    1981-01-01

    Experiments concerning the physical mechanisms for the abiotic generation and chemical mechanisms for the amplification of optical activity in biological compounds are reviewed. Attention is given to experiments involving the determination of the differential adsorption of racemic amino acids on d- and l-quartz, the asymmetric photolysis of racemic amino acids by circularly polarized light, and the asymmetric radiolysis of solid amino acids by longitudinally polarized electrons, and the enantiomeric enrichments thus obtained are noted. Further experiments on the amplification of the chirality in the polymerization of D, L-amino acid mixtures and the hydrolysis of D-, L-, and D, L-polypeptides are discussed. It is suggested that a repetitive cycle of partial polymerization-hydrolyses may account for the abiotic genesis of optically enriched polypeptides on the primitive earth.

  3. Abiotic stress QTL in lettuce crop–wild hybrids: comparing greenhouse and field experiments

    PubMed Central

    Hartman, Yorike; Hooftman, Danny A P; Uwimana, Brigitte; Schranz, M Eric; van de Wiel, Clemens C M; Smulders, Marinus J M; Visser, Richard G F; Michelmore, Richard W; van Tienderen, Peter H

    2014-01-01

    The development of stress-tolerant crops is an increasingly important goal of current crop breeding. A higher abiotic stress tolerance could increase the probability of introgression of genes from crops to wild relatives. This is particularly relevant to the discussion on the risks of new GM crops that may be engineered to increase abiotic stress resistance. We investigated abiotic stress QTL in greenhouse and field experiments in which we subjected recombinant inbred lines from a cross between cultivated Lactuca sativa cv. Salinas and its wild relative L. serriola to drought, low nutrients, salt stress, and aboveground competition. Aboveground biomass at the end of the rosette stage was used as a proxy for the performance of plants under a particular stress. We detected a mosaic of abiotic stress QTL over the entire genome with little overlap between QTL from different stresses. The two QTL clusters that were identified reflected general growth rather than specific stress responses and colocated with clusters found in earlier studies for leaf shape and flowering time. Genetic correlations across treatments were often higher among different stress treatments within the same experiment (greenhouse or field), than among the same type of stress applied in different experiments. Moreover, the effects of the field stress treatments were more correlated with those of the greenhouse competition treatments than to those of the other greenhouse stress experiments, suggesting that competition rather than abiotic stress is a major factor in the field. In conclusion, the introgression risk of stress tolerance (trans-)genes under field conditions cannot easily be predicted based on genomic background selection patterns from controlled QTL experiments in greenhouses, especially field data will be needed to assess potential (negative) ecological effects of introgression of these transgenes into wild relatives. PMID:25360276

  4. Abiotic and biotic controls of soil moisture spatiotemporal variability and the occurrence of hysteresis

    NASA Astrophysics Data System (ADS)

    Fatichi, Simone; Katul, Gabriel G.; Ivanov, Valeriy Y.; Pappas, Christoforos; Paschalis, Athanasios; Consolo, Ada; Kim, Jongho; Burlando, Paolo

    2015-05-01

    An expression that separates biotic and abiotic controls on the temporal dynamics of the soil moisture spatial coefficient of variation Cv(θ) was explored via numerical simulations using a mechanistic ecohydrological model, Tethys-Chloris. Continuous soil moisture spatiotemporal dynamics at an exemplary hillslope domain were computed for six case studies characterized by different climate and vegetation cover and for three configurations of soil properties. It was shown that abiotic controls largely exceed their biotic counterparts in wet climates. Biotic controls on Cv(θ) were found to be more pronounced in Mediterranean climates. The relation between Cv(θ) and spatial mean soil moisture θ¯ was found to be unique in wet locations, regardless of the soil properties. For the case of homogeneous soil texture, hysteretic cycles between Cv(θ) and θ¯ were observed in all Mediterranean climate locations considered here and to a lesser extent in a deciduous temperate forest. Heterogeneity in soil properties increased Cv(θ) to values commensurate with field observations and weakened signatures of hysteresis at all of the studied locations. This finding highlights the role of site-specific heterogeneities in hiding or even eliminating the signature of climatic and biotic controls on Cv(θ), thereby offering a new perspective on causes of confounding results reported across field experiments.

  5. Exploring biotic vs. abiotic controls on syngenetic carbonate and clay mineral precipitation

    NASA Astrophysics Data System (ADS)

    Nascimento, Gabriela S.; McKenzie, Judith A.; Martinez Ruiz, Francisca; Bontognali, Tomaso R. R.; Vasconcelos, Crisogono

    2016-04-01

    A possible syngenetic relationship between carbonate and clay mineral precipitation has been reported for sedimentary rocks deposited in both lacustrine and marine sedimentary environments throughout the geological record. In particular, the mineral dolomite is often found associated with Mg-rich clays, such as stevensite. It is notable that this carbonate/clay association has been recorded in numerous samples taken from modern dolomite precipitating environments; for example, the Coorong lakes, South Australia, coastal sabkhas, Abu Dhabi, UAE and coastal hypersaline lagoons (Lagoa Vermelha and Brejo do Espinho) east of Rio de Janeiro, Brazil. An HRTEM study of samples from these three locations indicates a possible physical/chemical association between the Ca-dolomite and Mg-rich clays, demonstrating a probable co-precipitation. To test this hypothesis, we have conducted a series of biotic and abiotic laboratory experiments. If this syngenesis actually occurs in nature, what, if any, are the biogeochemical processes controlling these precipitation reactions? Our experiments were designed to determine the extent of the biotic versus abiotic component influencing the mineral precipitation and, in the case of a biotic influence, to understand the mechanism through which microorganisms might mediate the formation of clay minerals. The experiments were carried out in the Geomicrobiology Laboratory of ETH Zürich using cultures of living microbes and artificial organic compounds that simulate functional groups present in natural biofilms formed under both aerobic and anaerobic conditions. In addition, pure inorganic experiments were designed to understand possible physico-chemical conditions for diagenetic processes that could induce dissolution of Mg-carbonates and precipitation of Mg-rich clays. Our results show a remarkable biotic influence during the formation of clay minerals. Specifically, extracellular polymeric substances (EPS), released by microbes in their

  6. Abiotic stress and control of grain number in cereals.

    PubMed

    Dolferus, Rudy; Ji, Xuemei; Richards, Richard A

    2011-10-01

    Grain number is the only yield component that is directly associated with increased grain yield in important cereal crops like wheat. Historical yield studies show that increases in grain yield are always accompanied by an increase in grain number. Adverse weather conditions can cause severe fluctuations in grain yield and substantial yield losses in cereal crops. The problem is global and despite its impact on world food production breeding and selection approaches have only met with limited success. A specific period during early reproductive development, the young microspore stage of pollen development, is extremely vulnerable to abiotic stress in self-fertilising cereals (wheat, rice, barley, sorghum). A better understanding of the physiological and molecular processes that lead to stress-induced pollen abortion may provide us with the key to finding solutions for maintaining grain number under abiotic stress conditions. Due to the complexity of the problem, stress-proofing our main cereal crops will be a challenging task and will require joint input from different research disciplines.

  7. Stable Carbon Isotopic Signatures of Abiotic Organics from Hydrothermal Synthesis Experiments

    NASA Technical Reports Server (NTRS)

    Stern, Jennifer C.; Summers, David P.; Kubo, Mike; Yassar, Saima

    2006-01-01

    Stable carbon isotopes can be powerful biogeochemical markers in the study of life's origins. Biogenic carbon fixation produces organics that are depleted in C-13 by about -20 to -30%0. Less attention has been paid to the isotopic signatures of abiotic processes. The possibility of abiotic processes producing organics with morphologies and isotopic signatures in the biogenic range has been at the center of recent debate over the Earth's earliest microfossils. The abiotic synthesis of organic compounds in hydrothermal environments is one possible source of endogenous organic matter to the prebiotic earth. Simulated hydrothermal settings have been shown to synthesize, among other things, single chain amphiphiles and simple lipids from a mix of CO, CO2, and H2. A key characteristic of these amphiphilic molecules is the ability to self-assemble in aqueous phases into more organized structures called vesicles, which form a selectively permeable boundary and serve the function of containing and concentrating other organic molecules. The ability to form cell like structures also makes these compounds more likely to be mistaken for biogenic. Hydrothermal simulation experiments were conducted from oxalic or formic acid in water at 175 C for 72 hr. The molecular and isotopic composition of the products of these reactions were determined and compared to biogenic fractionations . Preliminary results indicate isotopic fractionation during abiotic hydrocarbon synthesis in hydrothermal environments is on par with biological carbon fixation.

  8. Differential kinetics and temperature dependence of abiotic and biotic processes controlling the environmental fate of TNT in simulated marine systems.

    PubMed

    Chappell, Mark A; Porter, Beth E; Price, Cynthia L; Pettway, Brad A; George, Robert D

    2011-08-01

    This work seeks to understand how the balance of abiotic and biotic kinetic processes in sediments control the residual concentration of TNT in marine systems after release from ocean-dumped source. Kinetics of TNT disappearance were followed using marine sediments at different temperatures and under both biotic and presumably abiotic conditions (through sodium azide addition). Sediments exhibiting the highest rate of TNT disappearance under biotic conditions also exhibited the highest sorption affinity for TNT under abiotic conditions. Significant temperature dependence in the abiotic processes was observed in the diffusion coefficient of TNT and not sediment sorption affinity. At higher temperature, kinetics of biotic processes outpaced abiotic processes, but at low temperature, kinetics of abiotic processes were much more significant. We concluded that the differential influence of temperature on the kinetics of abiotic and biotic processes could provide distinguishing predictions for the potential residual concentration of TNT contamination in marine-sediment systems.

  9. Biotic and abiotic controls of argentine ant invasion success at local and landscape scales

    USGS Publications Warehouse

    Menke, S.B.; Fisher, R.N.; Jetz, W.; Holway, D.A.

    2007-01-01

    Although the ecological success of introduced species hinges on biotic interactions and physical conditions, few experimental studies - especially on animals - have simultaneously investigated the relative importance of both types of factors. The lack of such research may stem from the common assumption that native and introduced species exhibit similar environmental tolerances. Here we combine experimental and spatial modeling approaches (1) to determine the relative importance of biotic and abiotic controls of Argentine ant (Linepithema humile) invasion success, (2) to examine how the importance of these factors changes with spatial scale in southern California (USA), and (3) to assess how Argentine ants differ from native ants in their environmental tolerances. A factorial field experiment that combined native ant removal with irrigation revealed that Argentine ants failed to invade any dry plots (even those lacking native ants) but readily invaded all moist plots. Native ants slowed the spread of Argentine ants into irrigated plots but did not prevent invasion. In areas without Argentine ants, native ant species showed variable responses to irrigation. At the landscape scale, Argentine ant occurrence was positively correlated with minimum winter temperature (but not precipitation), whereas native ant diversity increased with precipitation and was negatively correlated with minimum winter temperature. These results are of interest for several reasons. First, they demonstrate that fine-scale differences in the physical environment can eclipse biotic resistance from native competitors in determining community susceptibility to invasion. Second, our results illustrate surprising complexities with respect to how the abiotic factors limiting invasion can change with spatial scale, and third, how native and invasive species can differ in their responses to the physical environment. Idiosyncratic and scale-dependent processes complicate attempts to forecast where

  10. Unveiling the Redox Control of Plant Reproductive Development during Abiotic Stress

    PubMed Central

    Zinta, Gaurav; Khan, Asif; AbdElgawad, Hamada; Verma, Vipasha; Srivastava, Ashish Kumar

    2016-01-01

    Plants being sessile in nature are often challenged to various abiotic stresses including temperature fluctuations, water supply, salinity, and nutrient availability. Exposure of plants to such environmental perturbations result in the formation of reactive oxygen species (ROS) in cells. To scavenge ROS, enzymatic and molecular antioxidants are produced at a cellular level. ROS act as a signaling entity at lower concentrations maintaining normal growth and development, but if their levels increase beyond certain threshold, they produce toxic effects in plants. Some developmental stages, such as development of reproductive organs are more sensitive to abiotic stress than other stages of growth. As success of plant reproductive development is directly correlated with grain yield, stresses coinciding with reproductive phase results in the higher yield losses. In this article, we summarize the redox control of plant reproductive development, and elaborate how redox homeostasis is compromised during abiotic stress exposure. We highlight why more emphasis should be given to understand redox control of plant reproductive organ development during abiotic stress exposure96to engineer crops with better crop yield. We specifically discuss the role of ROS as a signaling molecule and its cross-talk with other signaling molecules such as hormones and sugars. PMID:27379102

  11. Abiotic and Biotic Mechanisms Controlling In Situ Remediation of NDMA

    DTIC Science & Technology

    2009-05-01

    Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions...information if it does not display a currently valid OMB control number. 1 . REPORT DATE MAY 2009 2. REPORT TYPE 3. DATES COVERED 00-00-2009 to 00...aqueous) did not promote NDMA degradation; an iron oxide or 2: 1 clay was needed for electron transfer. NDMA degradation to DMA under iron-reducing

  12. SERDP ER-1421 Abiotic and Biotic Mechanisms Controlling In Situ Remediation of NDMA: Final Report

    SciTech Connect

    Szecsody, James E.; McKinley, James P.; Crocker, Fiona H.; Breshears, Andrew T.; Devary, Brooks J.; Fredrickson, Herbert L.; Thompson, Karen T.

    2009-09-30

    This laboratory-scale project was initiated to investigate in situ abiotic/biotic mineralization of NDMA. Under iron-reducing conditions, aquifer sediments showed rapid abiotic NDMA degradation to dimethylamine (DMA), nitrate, formate, and finally, CO2. These are the first reported experiments of abiotic NDMA mineralization. The NDMA reactivity of these different iron phases showed that adsorbed ferrous iron was the dominant reactive phase that promoted NDMA reduction, and other ferrous phases present (siderite, iron sulfide, magnetite, structural ferrous iron in 2:1 clays) did not promote NDMA degradation. In contrast, oxic sediments that were biostimulated with propane promoted biomineralization of NDMA by a cometabolic monooxygenase enzyme process. Other monooxygenase enzyme processes were not stimulated with methane or toluene additions, and acetylene addition did not block mineralization. Although NDMA mineralization extent was the highest in oxic, biostimulated sediments (30 to 82%, compared to 10 to 26% for abiotic mineralization in reduced sediments), large 1-D column studies (high sediment/water ratio of aquifers) showed 5.6 times higher NDMA mineralization rates in reduced sediment (half-life 410 ± 147 h) than oxic biomineralization (half life 2293 ± 1866 h). Sequential reduced/oxic biostimulated sediment mineralization (half-life 3180 ± 1094 h) was also inefficient compared to reduced sediment. These promising laboratory-scale results for NDMA mineralization should be investigated at field scale. Future studies of NDMA remediation should focus on the comparison of this in situ abiotic NDMA mineralization (iron-reducing environments) to ex situ biomineralization, which has been shown successful in other studies.

  13. Biotic control of an abiotic resource: Palmyra Atoll, a case study

    NASA Astrophysics Data System (ADS)

    Engels, M.; Heinse, R.

    2013-12-01

    The availability of abiotic resources as a control on the spatial distribution of plants in an ecosystem is well studied. Less well known is the impact of plants on the availability of abiotic resources and how this biotic control of the environment influences vegetative distribution over time. In order to investigate this question, we examined the influence of vegetation on the water balance of a tropical atoll. Palmyra is a small (2.5 km2) wet atoll in the northern Line Islands that hosts five species of native and introduced trees/shrubs, Cocos nucifera, Pisonia grandis, Tournefortia argentia, Terminalia catappa, and Scaevola sericea, accounting for ~85% percent of the vegetative coverage on the island. Using historical aerial photographs, climate data, published estimates of vegetative cover, and published estimates of transpiration rates we present estimates of the range of vegetative water demand and the change in water demand as influenced by changes in vegetation distribution through time.

  14. Subsoil Soil Organic Matter Complexation and Stabilization: Assessment of Abiotic and Biotic Controls

    NASA Astrophysics Data System (ADS)

    Rosier, C. L.; Kan, J.; Aufdenkampe, A. K.; Yoo, K.

    2011-12-01

    Approximately 1200-2000 petagrams (Pg-1015 g) of carbon are stored in the Earth's soil as soil organic matter (SOM), representing two times the amount of carbon stored in the Earth's vegetation and atmosphere combined. SOM significantly influences several essential ecosystem services including nutrient cycling, mitigation of soil erosion, and storage of atmospheric CO2. The majority of studies investigating SOM complexation and stabilization potential mainly occur within the A soil horizon completely ignoring deeper soil horizons. Studies aimed at investigating specific abiotic and biotic interactions that facilitate the complexation and stabilization potential of SOM to C-limited subsoil horizons are needed in order to develop an accurate soil carbon budget. The purpose of this study was to determine the degree to which the presence or absence of iron oxide in combination with increasing degrees of biological processing (micro-macrofauna) would complex and stabilize SOM. We conducted a series of laboratory soil incubation experiments using carbon amended B horizon soils with low and high iron oxide concentrations with increasing levels of biological processing. The experimental design of our study allowed us to track the possible fate of soil carbon: (i) CO2 mineralization (modified Li-COR), (ii) particulate organic matter (density fractionization), mineral surface complexed carbon (N2 adsorption BET method) and (iii) organism biomass. Results from our study clearly demonstrate that the greater the degree of macro-scale biological processing (i.e. mixing) in conjunction with the presence of iron oxide significantly increased the complexation and stabilization potential of SOM. Our results further suggest that organic matter interaction with mineral surfaces and entombment within stable soil aggregates were the primary mechanisms controlling SOM storage. This study reveals the importance of biological SOM burial and mixing with C-limited subsoil horizons as a

  15. Abiotic controls of emergent macrophyte density in a bedrock channel - The Cahaba River, AL (USA)

    NASA Astrophysics Data System (ADS)

    Vaughn, Ryan S.; Davis, Lisa

    2015-10-01

    Research examining bedrock channels is growing. Despite this, biotic-abiotic interactions remain a topic mostly addressed in alluvial systems. This research identified hydrogeomorphic factors operating at the patch-scale (100-102 m) in bedrock shoals of the Cahaba River (AL) that help determine the distribution of the emergent aquatic macrophyte, Justicia americana. Macrophyte patch density (number of stems/m2) and percent bedrock void surface area (rock surface area/m2 occupied by joints, fractures, and potholes) were measured (n = 24 within two bedrock shoals) using stem counts and underwater photography, respectively. One-dimensional hydrologic modeling (HEC-RAS 4.1.0) was completed for a section within a shoal to examine velocity and channel depth as controlling variables for macrophyte patch density. Results from binary logistic regression analysis identified depth and velocity as good predictors of the presence or absence of Justicia americana within shoal structures (depth p = 0.001, velocity p = 0.007), which is a similar finding to previous research conducted in alluvial systems. Correlation analysis between bedrock surface void area and stem density demonstrated a statistically significant positive correlation (r = 0.665, p = 0.01), elucidating a link between abiotic-biotic processes that may well be unique to bedrock channels. These results suggest that the amount of void space present in bedrock surfaces, in addition to localized depth and velocity, helps control macrophyte patch density in bedrock shoal complexes. The utility of geomorphology in explaining patch-scale habitat heterogeneity in this study highlights geomorphology's potential to help understand macrophyte habitat heterogeneity at the reach scale, while also demonstrating its promise for mapping and understanding habitat heterogeneity at the system scale.

  16. Factors controlling the abiotic photo-degradation of monomethylmercury in surface waters

    NASA Astrophysics Data System (ADS)

    Black, Frank J.; Poulin, Brett A.; Flegal, A. Russell

    2012-05-01

    Photo-decomposition is among the most important mechanisms responsible for degrading monomethylmercury (MMHg) in aquatic systems, but this process is not fully understood. We investigated the relative importance of different factors in controlling the rate of MMHg photo-decomposition in surface waters in experiments using DOM isolated from natural waters. We found no evidence of net abiotic production of MMHg in any dark or light exposed treatments. The average (mean ± s.d.) MMHg photo-decomposition rate constant for all light exposed samples using DOM concentrated from three coastal wetlands was 0.0099 ± 0.0020 E-1m2 (range of 0.006-0.015 E-1m2) when expressed in photon flux from 330-700 nm. This was roughly 3-fold higher than the average MMHg photo-decomposition rate constant in coastal seawater of 0.0032 ± 0.0010 E-1m2. MMHg photo-degradation was highly wavelength dependent. The ratio of MMHg photo-decomposition rate constants, with respect to photon flux, was 400:37:1 for UVB:UVA:PAR. However, when integrated across the entire water column over which MMHg photo-demethylation occurs, PAR was responsible for photo-degrading more MMHg than UVB and UVA combined in the three wetland sites because of the more rapid attenuation of UV light with depth. MMHg half-lives in the wetlands were calculated for the upper 250 cm where photo-degradation occurred, and ranged from 7.6 to 20 days under typical summer sunlight conditions at 37°N. Rates of MMHg photo-decomposition decreased with increasing salinity, and were 27% higher at a salinity of 5 than those at a salinity of 25. This difference could not be accounted for by changes in the complexation of MMHg by DOM and chloride. Differences in MMHg photo-degradation rate constants of up to 18% were measured between treatments using DOM concentrated from three different wetlands. Surprisingly, increasing DOM concentration from 1.5 to 11.3 mg OC L-1 had only a small (6%) effect on MMHg photo-decomposition, which was much

  17. Chemical controls on abiotic and biotic release of geogenic arsenic from Pleistocene aquifer sediments to groundwater.

    PubMed

    Gillispie, Elizabeth C; Andujar, Erika; Polizzotto, Matthew L

    2016-08-10

    Over 150 million people in South and Southeast Asia consume unsafe drinking water from arsenic-rich Holocene aquifers. Although use of As-free water from Pleistocene aquifers is a potential mitigation strategy, such aquifers are vulnerable to geogenic As pollution, placing millions more people at potential risk. The goal of this research was to define chemical controls on abiotic and biotic release of geogenic As to groundwater. Batch incubations of sediments with natural chemical variability from a Pleistocene aquifer in Cambodia were conducted to evaluate how interactions among arsenic, manganese and iron oxides, and dissolved and sedimentary organic carbon influenced As mobilization from sediments. The addition of labile dissolved organic carbon produced the highest concentrations of dissolved As after >7 months, as compared to sediment samples incubated with sodium azide or without added carbon, and the extent of As release was positively correlated with the percent of initial extractable Mn released from the sediments. The mode of As release was impacted by the source of DOC supplied to the sediments, with biological processes responsible for 81% to 85% of the total As release following incubations with lactate and acetate but only up to 43% to 61% of the total As release following incubations with humic and fulvic acids. Overall, cycling of key redox-active elements and organic-carbon reactivity govern the potential for geogenic As release to groundwater, and results here may be used to formulate better predictions of the arsenic pollution potential of aquifers in South and Southeast Asia.

  18. Polyamines control of cation transport across plant membranes: implications for ion homeostasis and abiotic stress signaling

    PubMed Central

    Pottosin, Igor; Shabala, Sergey

    2014-01-01

    Polyamines are unique polycationic metabolites, controlling a variety of vital functions in plants, including growth and stress responses. Over the last two decades a bulk of data was accumulated providing explicit evidence that polyamines play an essential role in regulating plant membrane transport. The most straightforward example is a blockage of the two major vacuolar cation channels, namely slow (SV) and fast (FV) activating ones, by the micromolar concentrations of polyamines. This effect is direct and fully reversible, with a potency descending in a sequence Spm4+ > Spd3+ > Put2+. On the contrary, effects of polyamines on the plasma membrane (PM) cation and K+-selective channels are hardly dependent on polyamine species, display a relatively low affinity, and are likely to be indirect. Polyamines also affect vacuolar and PM H+ pumps and Ca2+ pump of the PM. On the other hand, catabolization of polyamines generates H2O2 and other reactive oxygen species (ROS), including hydroxyl radicals. Export of polyamines to the apoplast and their oxidation there by available amine oxidases results in the induction of a novel ion conductance and confers Ca2+ influx across the PM. This mechanism, initially established for plant responses to pathogen attack (including a hypersensitive response), has been recently shown to mediate plant responses to a variety of abiotic stresses. In this review we summarize the effects of polyamines and their catabolites on cation transport in plants and discuss the implications of these effects for ion homeostasis, signaling, and plant adaptive responses to environment. PMID:24795739

  19. Biotic, abiotic and management controls on methanol exchange above a temperate mountain grassland

    PubMed Central

    Hörtnagl, Lukas; Bamberger, Ines; Graus, Martin; Ruuskanen, Taina M.; Schnitzhofer, Ralf; Müller, Markus; Hansel, Armin; Wohlfahrt, Georg

    2013-01-01

    Methanol (CH3OH) fluxes were quantified above a managed temperate mountain grassland in the Stubai Valley (Tyrol, Austria) during the growing seasons 2008 and 2009. Half-hourly methanol fluxes were calculated by means of the virtual disjunct eddy covariance (vDEC) method using 3-dimensional wind data from a sonic anemometer and methanol volume mixing ratios measured with a proton-transfer-reaction mass spectrometer (PTR-MS). During (undisturbed) mature and growing phases methanol fluxes exhibited a clear diurnal cycle with close-to-zero fluxes during nighttime and emissions, up to 10 nmol m−2 s−1, which followed the diurnal course of radiation and air temperature. Management events were found to represent the largest perturbations of methanol exchange at the studied grassland ecosystem: Peak emissions of 144.5 nmol m−2 s−1 were found during/after cutting of the meadow reflecting the wounding of the plant material and subsequent depletion of the leaf internal aqueous methanol pools. After the application of organic fertilizer, elevated methanol emissions of up to 26.7 nmol m−2 s−1 were observed, likely reflecting enhanced microbial activity associated with the applied manure. Simple and multiple linear regression analyses revealed air temperature and radiation as the dominant abiotic controls, jointly explaining 47 % and 70 % of the variability in half-hourly and daily methanol fluxes. In contrast to published leaf-level laboratory studies, the surface conductance and the daily change in the amount of green plant area, used as ecosystem-scale proxies for stomatal conductance and growth, respectively, were found to exert only minor biotic controls on methanol exchange. PMID:24349901

  20. Abiotic versus biotic controls on soil nitrogen cycling in drylands along a 3200 km transect

    NASA Astrophysics Data System (ADS)

    Liu, Dongwei; Zhu, Weixing; Wang, Xiaobo; Pan, Yuepeng; Wang, Chao; Xi, Dan; Bai, Edith; Wang, Yuesi; Han, Xingguo; Fang, Yunting

    2017-03-01

    Nitrogen (N) cycling in drylands under changing climate is not well understood. Our understanding of N cycling over larger scales to date relies heavily on the measurement of bulk soil N, and the information about internal soil N transformations remains limited. The 15N natural abundance (δ15N) of ammonium and nitrate can serve as a proxy record for the N processes in soils. To better understand the patterns and mechanisms of N cycling in drylands, we collected soils along a 3200 km transect at about 100 km intervals in northern China, with mean annual precipitation (MAP) ranging from 36 to 436 mm. We analyzed N pools and δ15N of ammonium, dual isotopes (15N and 18O) of nitrate, and the microbial gene abundance associated with soil N transformations. We found that N status and its driving factors were different above and below a MAP threshold of 100 mm. In the arid zone with MAP below 100 mm, soil inorganic N accumulated, with a large fraction being of atmospheric origin, and ammonia volatilization was strong in soils with high pH. In addition, the abundance of microbial genes associated with soil N transformations was low. In the semiarid zone with MAP above 100 mm, soil inorganic N concentrations were low and were controlled mainly by biological processes (e.g., plant uptake and denitrification). The preference for soil ammonium over nitrate by the dominant plant species may enhance the possibility of soil nitrate losses via denitrification. Overall, our study suggests that a shift from abiotic to biotic controls on soil N biogeochemistry under global climate changes would greatly affect N losses, soil N availability, and other N transformation processes in these drylands in China.

  1. Global Patterns of Stem CO2 Efflux in Forests and its Biotic and Abiotic Controls

    NASA Astrophysics Data System (ADS)

    Yang, J.

    2015-12-01

    Forest stem CO2 efflux (ES) plays an important role in the carbon balance of forest ecosystems. However, its primary controls at the global scale are poorly understood and observation-based global estimates are lacking. We synthesized data from XX published studies across global forest ecosystems and examined the relationships between annual ES and abiotic factors (mean annual temperature (MAT), mean annual precipitation (MAP)) and biotic factors (gross primary production (GPP), net primary production (NPP), leaf area index (LAI), soil CO2 efflux (RS), root CO2 efflux (RA), foliar CO2 efflux, basal area, and stand age). Based on our compiled datasets, the mean annual ES was 90 ± 43 g C m-2 yr-1, 160 ± 79 g C m-2 yr-1 and 501 ± 363 g C m-2 yr-1 for boreal, temperate, and tropical forest ecosystems, respectively. Average apparent Q10 of ES for boreal forests was 1.85, 2.29 for temperate forests, and 2.26 for tropical forests. Globally, MAT and MAP explained 32% and 24% of the variation in annual ES, respectively. Positive linear relationships exist between ES and forest production (GPP and NPP), LAI, RS, and RA in different forest ecosystems. These correlations hold for the global dataset, as well as for temperate and tropical forest ecosystems independently. In addition, we found no correlation between ES and both RS and RA after accounting for temperature and GPP effects. The predicted a global annual ES was about 6.1Pg C yr-1.

  2. Form and function of grass ring patterns in arid grasslands: the role of abiotic controls.

    PubMed

    Ravi, Sujith; D'Odorico, Paolo; Wang, Lixin; Collins, Scott

    2008-12-01

    Ring-shaped growth patterns commonly occur in resource-limited arid and semi-arid environments. The spatial distribution, geometry, and scale of vegetation growth patterns result from interactions between biotic and abiotic processes, and, in turn, affect the spatial patterns of soil moisture, sediment transport, and nutrient dynamics in aridland ecosystems. Even though grass ring patterns are observed worldwide, a comprehensive understanding of the biotic and abiotic processes that lead to the formation, growth and breakup of these rings is still lacking. Our studies on patterns of infiltration and soil properties of blue grama (Bouteloua gracilis) grass rings in the northern Chihuahuan desert indicate that ring patterns result from the interaction between clonal growth mechanisms and abiotic factors such as hydrological and aeolian processes. These processes result in a negative feedback between sediment deposition and vegetation growth inside the bunch grass, which leads to grass die back at the center of the grass clump. We summarize these interactions in a simple theoretical and conceptual model that integrates key biotic and abiotic processes in ring formation, growth and decline.

  3. Authentic Research Experience and "Big Data" Analysis in the Classroom: Maize Response to Abiotic Stress.

    PubMed

    Makarevitch, Irina; Frechette, Cameo; Wiatros, Natalia

    2015-01-01

    Integration of inquiry-based approaches into curriculum is transforming the way science is taught and studied in undergraduate classrooms. Incorporating quantitative reasoning and mathematical skills into authentic biology undergraduate research projects has been shown to benefit students in developing various skills necessary for future scientists and to attract students to science, technology, engineering, and mathematics disciplines. While large-scale data analysis became an essential part of modern biological research, students have few opportunities to engage in analysis of large biological data sets. RNA-seq analysis, a tool that allows precise measurement of the level of gene expression for all genes in a genome, revolutionized molecular biology and provides ample opportunities for engaging students in authentic research. We developed, implemented, and assessed a series of authentic research laboratory exercises incorporating a large data RNA-seq analysis into an introductory undergraduate classroom. Our laboratory series is focused on analyzing gene expression changes in response to abiotic stress in maize seedlings; however, it could be easily adapted to the analysis of any other biological system with available RNA-seq data. Objective and subjective assessment of student learning demonstrated gains in understanding important biological concepts and in skills related to the process of science.

  4. Habitat fragmentation as a result of biotic and abiotic factors controls pathogen transmission throughout a host population.

    PubMed

    Greer, Amy L; Collins, James P

    2008-03-01

    1. The hypothesis that habitat fragmentation (biotic or abiotic) alters the transmission of disease within a population is explored using field data from a well-studied amphibian-pathogen system. 2. We used the Ambystoma tigrinum-A. tigrinum virus (ATV) model system to show how habitat fragmentation as a result of emergent vegetation and habitat management affects disease transmission dynamics in ponds across a landscape. 3. We quantified variation in ATV infection over time and across the landscape. ATV infection was significantly higher in ponds modified for livestock use (P = 0.032). Disease incidence decreased with increased amounts of emergent vegetation (P < 0.001). These factors appear to control disease transmission by altering the host contact rate and with it disease transmission. 4. A field experiment to test the effect of emergent vegetation on the distribution of larvae in ponds demonstrated a behavioural change in larvae found in sparsely vegetated ponds. Microhabitat choices resulted in larvae being concentrated at the pond edge resulting in a 'halo effect' in sparsely vegetated ponds, whereas larvae in heavily vegetated ponds were distributed more evenly throughout. Microhabitat choice affects the effective density that larvae experience. This 'halo effect' increases contact rates in the shallows of sparsely vegetated ponds and increases the transmission of a directly transmitted pathogen. 5. Despite recurrent epidemics of a lethal Ranavirus in tiger salamanders on the Kaibab Plateau, Arizona, USA, these populations persist. We discuss the implications of our results in the context of density-dependent transmission and homogeneous mixing, two increases key assumptions of epidemiological theory.

  5. Biotic and abiotic controls on co-occurring nitrogen cycling processes in shallow Arctic shelf sediments

    NASA Astrophysics Data System (ADS)

    McTigue, N. D.; Gardner, W. S.; Dunton, K. H.; Hardison, A. K.

    2016-10-01

    The processes that convert bioavailable inorganic nitrogen to inert nitrogen gas are prominent in continental shelf sediments and represent a critical global sink, yet little is known of these pathways in the Arctic where 18% of the world's continental shelves are located. Moreover, few data from the Arctic exist that separate loss processes like denitrification and anaerobic ammonium oxidation (anammox) from recycling pathways like dissimilatory nitrate reduction to ammonium (DNRA) or source pathways like nitrogen fixation. Here we present measurements of these co-occurring processes using 15N tracers. Denitrification was heterogeneous among stations and an order of magnitude greater than anammox and DNRA, while nitrogen fixation was undetectable. No abiotic factors correlated with interstation variability in biogeochemical rates; however, bioturbation potential explained most of the variation. Fauna-enhanced denitrification is a potentially important but overlooked process on Arctic shelves and highlights the role of the Arctic as a significant global nitrogen sink.

  6. Biotic and abiotic controls on co-occurring nitrogen cycling processes in shallow Arctic shelf sediments

    PubMed Central

    McTigue, N. D.; Gardner, W. S.; Dunton, K. H.; Hardison, A. K.

    2016-01-01

    The processes that convert bioavailable inorganic nitrogen to inert nitrogen gas are prominent in continental shelf sediments and represent a critical global sink, yet little is known of these pathways in the Arctic where 18% of the world's continental shelves are located. Moreover, few data from the Arctic exist that separate loss processes like denitrification and anaerobic ammonium oxidation (anammox) from recycling pathways like dissimilatory nitrate reduction to ammonium (DNRA) or source pathways like nitrogen fixation. Here we present measurements of these co-occurring processes using 15N tracers. Denitrification was heterogeneous among stations and an order of magnitude greater than anammox and DNRA, while nitrogen fixation was undetectable. No abiotic factors correlated with interstation variability in biogeochemical rates; however, bioturbation potential explained most of the variation. Fauna-enhanced denitrification is a potentially important but overlooked process on Arctic shelves and highlights the role of the Arctic as a significant global nitrogen sink. PMID:27782213

  7. Abiotic and biotic controls on local spatial distribution and performance of Boechera stricta.

    PubMed

    Naithani, Kusum J; Ewers, Brent E; Adelman, Jonathan D; Siemens, David H

    2014-01-01

    This study investigates the relative influence of biotic and abiotic factors on community dynamics using an integrated approach and highlights the influence of space on genotypic and phenotypic traits in plant community structure. We examined the relative influence of topography, environment, spatial distance, and intra- and interspecific interactions on spatial distribution and performance of Boechera stricta (rockcress), a close perennial relative of model plant Arabidopsis. First, using Bayesian kriging, we mapped the topography and environmental gradients and explored the spatial distribution of naturally occurring rockcress plants and two neighbors, Taraxacum officinale (dandelion) and Solidago missouriensis (goldenrod) found in close proximity within a typical diverse meadow community across topographic and environmental gradients. We then evaluated direct and indirect relationships among variables using Mantel path analysis and developed a network displaying abiotic and biotic interactions in this community. We found significant spatial autocorrelation among rockcress individuals, either because of common microhabitats as displayed by high density of individuals at lower elevation and high soil moisture area, or limited dispersal as shown by significant spatial autocorrelation of naturally occurring inbred lines, or a combination of both. Goldenrod and dandelion density around rockcress does not show any direct relationship with rockcress fecundity, possibly due to spatial segregation of resources. However, dandelion density around rockcress shows an indirect negative influence on rockcress fecundity via herbivory, indicating interspecific competition. Overall, we suggest that common microhabitat preference and limited dispersal are the main drivers for spatial distribution. However, intra-specific interactions and insect herbivory are the main drivers of rockcress performance in the meadow community.

  8. The influence of abiotic controls and management intensity on phosphorus cycling in established grassland and forest ecosystems

    NASA Astrophysics Data System (ADS)

    Alt, F.; Oelmann, Y.; Wilcke, W.

    2011-12-01

    It is commonly assumed that the bioavailability and cycling of phosphorus (P) is mainly controlled by abiotic soil properties including soil pH and the concentrations and reactivities of clay minerals, CaCO3 and Al/Fe oxides In managed ecosystems, kind, timing and duration of P additions and type and amount of harvested biomass are the major input and output fluxes. Our objective was to disentangle the effects of abiotic controls, and type and intensity of management on the P cycle in soils of temperate grasslands and forests of different management intensity in three regions across Germany in the frame of the Biodiversity Exploratories project. The pH value was the most important variable explaining P concentrations and partitioning in soil and changes in pH are the main mechanism how land-use is affecting the P cycle. However, after the influence of pH was accounted for in a sequential statistical approach, land-use intensity, classified according to the extent of annual biomass removal, explained a significant (P < 0.05) part of the variance in the contributions of several P fractions to total P (TP) among all studied regions and land-use types. In grassland soils of highly diverse systems (up to 57 plant species) in one of the study regions, the Schwäbische Alb, a mid-range mountain area on limestone where soils showed a limited variation in pH in the carbonate buffer range, pedogenic Fe oxide concentrations, fertilizer-P application rates, and TP concentrations in soil explained more than half of the variation in bioavailable inorganic (Pi) concentrations extracted with NaHCO3 in soil. Our results demonstrate that mainly soil pH and mineralogical composition, and intensity of management of the managed ecosystems are significant controls of the P cycle determining the size of bioavailable P pool in soil.

  9. Abiotic formation of bioorganic compounds in space--preliminary experiments on ground and future exobiology experiments in space.

    PubMed

    Kobayashi, K; Kaneko, T; Hashimoto, H; Kouchi, A; Saito, T; Yamashita, M

    1998-06-01

    Simulation experiments on ground have shown that "amino acid precursors", which give amino acids after acid-hydrolysis, can be formed when an ice mixture simulating ice mantles of interstellar dust particles (lSDs) is irradiated with high energy particles or UV light. It is strongly suggested that such bioorganic compounds were delivered by comets for the first biosphere on the Earth. It is of great interest to confirm this hypothesis in actual space conditions, such as in an exposed facility of JEM. Fundamental designs for such exobiology experiments in earth orbit (EEEO) will be discussed.

  10. Abiotic and biotic controls of cryptobenthic fish assemblages across a Caribbean seascape

    NASA Astrophysics Data System (ADS)

    Harborne, A. R.; Jelks, H. L.; Smith-Vaniz, W. F.; Rocha, L. A.

    2012-12-01

    The majority of fish studies on coral reefs consider only non-cryptic species and, despite their functional importance, data on cryptic species are scarce. This study investigates inter-habitat variation in Caribbean cryptobenthic fishes by re-analysing a comprehensive data set from 58 rotenone stations around Buck Island, U.S. Virgin Islands. Boosted regression trees were used to associate the density and diversity of non-piscivorous cryptobenthic fishes, both in the entire data set and on reef habitats alone, with 14 abiotic and biotic variables. The study also models the habitat requirements of the three commonest species. Dead coral cover was the first or second most important variable in six of the eight models constructed. For example, within the entire data set, the number of species and total fish density increased approximately linearly with increasing dead coral cover. Dead coral was also important in multivariate analyses that discriminated 10 assemblages within the entire data set. On reef habitats, the number of species and total fish density increased dramatically when dead coral exceeded ~55 %. Live coral cover was typically less important for explaining variance in fish assemblages than dead coral, but live corals were important for maintaining high fish diversity. Coral species favoured by cryptobenthic species may be particularly susceptible to mortality, but dead coral may also provide abundant food and shelter for many fishes. Piscivore density was a key variable in the final models, but typically increased with increasing cryptobenthic fish diversity and abundance, suggesting both groups of fishes are responding to the same habitat variables. The density of territorial damselfishes reduced the number of cryptobenthic fish species on reef habitats. Finally, habitats delineated by standard remote sensing techniques supported distinct cryptobenthic fish assemblages, suggesting that such maps can be used as surrogates of general patterns of cryptic

  11. Biotic and abiotic controls on diurnal fluctuations in labile soil phosphorus of a wet tropical forest.

    PubMed

    Vandecar, Karen L; Lawrence, Deborah; Wood, Tana; Oberbauer, Steven F; Das, Rishiraj; Tully, Katherine; Schwendenmann, Luitgard

    2009-09-01

    The productivity of many tropical wet forests is generally limited by bioavailable phosphorus (P). Microbial activity is a key regulator of P availability in that it determines both the supply of P through organic matter decomposition and the depletion of bioavailable P through microbial uptake. Both microbial uptake and mineralization occur rapidly, and their net effect on P availability varies with soil moisture, temperature, and soil organic matter quantity and quality. Exploring the mechanisms driving P availability at fine temporal scales can provide insight into the coupling of carbon, water, and nutrient cycles, and ultimately, the response of tropical forests to climate change. Despite the recognized importance of P cycling to the dynamics of wet tropical forests and their potential sensitivity to short-term fluctuations in bioavailable P, the diurnal pattern of P remains poorly understood. This study quantifies diurnal fluctuations in labile soil P and evaluates the importance of biotic and abiotic factors in driving these patterns. To this end, measurements of labile P were made every other hour in a Costa Rican wet tropical forest oxisol. Spatial and temporal variation in Bray-extractable P were investigated in relation to ecosystem carbon flux, soil CO2 efflux, soil moisture, soil temperature, solar radiation, and sap-flow velocity. Spatially averaged bi-hourly (every two hours) labile P ranged from 0.88 to 2.48 microg/g across days. The amplitude in labile P throughout the day was 0.61-0.82 microg/g (41-54% of mean P concentrations) and was characterized by a bimodal pattern with a decrease at midday. Labile P increased with soil CO2 efflux and soil temperature and declined with increasing sap flow and solar radiation. Together, soil CO2 efflux, soil temperature, and sap flow explained 86% of variation in labile P.

  12. Gamma irradiation: a method to produce an abiotic control for biological activated carbon.

    PubMed

    Stoquart, C; Vázquez-Rodríguez, G A; Servais, P; Barbeau, B

    2013-01-01

    The aim of this paper was to investigate the feasibility of using gamma irradiation to inhibit the microbial activity of biological powder activated carbon (PAC) without impacting its adsorptive properties. First of all, the range of dose of gamma rays required to produce abiotic PAC was selected on the basis of heterotrophic plate counts (HPC) inactivation and methylene blue (MB) adsorption kinetics. Doses inferior to 10 kGy were not sufficient to inhibit the culture of heterotrophic bacteria. On the other hand, doses superior to 15 kGy were demonstrated to affect the adsorption rate of MB. Consequently, a dose comprised between 10 and 15 kGy was selected for further investigation. In order to validate the adequacy of the range of dose (i.e. 10-15 kGy), adsorption characteristics were tested by monitoring the removal kinetics of refractory dissolved organic carbon (RDOC). No significant differences were observed between irradiated and non-irradiated biological PAC for the adsorption of RDOC. Irradiated, non-irradiated and virgin PAC were also evaluated in terms of abundance of viable (using the LIVE/DEAD BacLight method) bacteria and in terms of heterotrophic biomass activity. The results of the BacLight method demonstrated that attachment of the biofilm on the PAC was not impacted by the irradiation and heterotrophic activity measurements demonstrated that the latter could be radically reduced in the range of dose selected. In conclusion, when using a proper dose, the gamma irradiation of colonized activated carbon drastically reduced the heterotrophic activity on activated carbon without significantly impacting its adsorptive behaviour.

  13. Abiotic and biotic controls of cryptobenthic fish assemblages across a Caribbean seascape

    USGS Publications Warehouse

    Harborne, A.R.; Jelks, H.L.; Smith-Vaniz, W.F.; Rocha, L.A.

    2012-01-01

    The majority of fish studies on coral reefs consider only non-cryptic species and, despite their functional importance, data on cryptic species are scarce. This study investigates inter-habitat variation in Caribbean cryptobenthic fishes by re-analysing a comprehensive data set from 58 rotenone stations around Buck Island, U.S. Virgin Islands. Boosted regression trees were used to associate the density and diversity of non-piscivorous cryptobenthic fishes, both in the entire data set and on reef habitats alone, with 14 abiotic and biotic variables. The study also models the habitat requirements of the three commonest species. Dead coral cover was the first or second most important variable in six of the eight models constructed. For example, within the entire data set, the number of species and total fish density increased approximately linearly with increasing dead coral cover. Dead coral was also important in multivariate analyses that discriminated 10 assemblages within the entire data set. On reef habitats, the number of species and total fish density increased dramatically when dead coral exceeded ~55 %. Live coral cover was typically less important for explaining variance in fish assemblages than dead coral, but live corals were important for maintaining high fish diversity. Coral species favoured by cryptobenthic species may be particularly susceptible to mortality, but dead coral may also provide abundant food and shelter for many fishes. Piscivore density was a key variable in the final models, but typically increased with increasing cryptobenthic fish diversity and abundance, suggesting both groups of fishes are responding to the same habitat variables. The density of territorial damselfishes reduced the number of cryptobenthic fish species on reef habitats. Finally, habitats delineated by standard remote sensing techniques supported distinct cryptobenthic fish assemblages, suggesting that such maps can be used as surrogates of general patterns of cryptic

  14. Abiotic processes are insufficient for fertile island development: A 10-year artificial shrub experiment in a desert grassland

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The relative importance of biotic and abiotic processes in the development of “fertile islands” in dryland systems has rarely been investigated. Here we approached this question by using artificial shrubs, which exclude plant litter production and soil nutrient uptake, but retain the functions of tr...

  15. Abiotic and biotic controls on methane formation down to 2.5 km depth within the Precambrian Fennoscandian Shield

    NASA Astrophysics Data System (ADS)

    Kietäväinen, Riikka; Ahonen, Lasse; Niinikoski, Paula; Nykänen, Hannu; Kukkonen, Ilmo T.

    2017-04-01

    Despite a geological history characterised by high temperature and pressure processes and organic carbon deprived crystalline bedrock, large amounts of hydrocarbons are found in deep groundwaters within Precambrian continental shields. In many sites, methane comprises more that 80% of the dissolved gas phase reaching concentrations of tens of mmol l-1. In this study, we used isotopic methods to study the carbon isotope systematics and sources of crustal methane within the Fennoscandian Shield. The main study sites were the Outokumpu Deep Drill Hole and the Pyhäsalmi mine in Finland, both of which allow groundwater sampling down to 2.5 km depth and have been previously studied for their groundwater chemistry and microbiology. We show that the differences in the amount and isotopic composition of methane are related to the availability of carbon sources as well as processes behind the incorporation of hydrogen and carbon via abiotic and biotic pathways into hydrocarbon molecules. Supported by previously reported occurrences and isotopic data of deep groundwater methane in lithologically different locations in Finland and Sweden, we show that methane formation is controlled by microbial methanogenesis and abiotic reactions, as well as lithology with the metasedimentary environments being the most favourable for methane occurrence. Rather than a thermogenic relic, crustal methane within the Fennoscandian Shield is more likely the result of low temperature formation from ancient organic compounds or their inorganic intermediates such as graphite. Such crustal gases are characterised by the lack of major amounts of C2+ hydrocarbons and 13C-rich methane. Further, microbiological and isotopic geochemical evidence suggest that microbial methane is more common at depths shallower than 1.5 km.

  16. Abiotic controls on H{sub 2} production from basalt-water reactions and implications for aquifer biogeochemistry

    SciTech Connect

    Stevens, T.O.; McKinley, J.P.

    2000-03-01

    Abiotic production of H{sub 2} from basalt reactions in aqueous solutions is hypothesized to support microbial ecosystems in deep subsurface aquifers, such as those found in the Columbia River Basalt group (CRB). The authors investigated factors controlling this phenomenon, including rock composition, pH, temperature, sterilization method, reducing agents, and product removal. Ferrous silicate minerals were found to be the basalt components responsible for H{sub 2} production from anaerobic water-rock interactions. H{sub 2} evolution was faster at pH < 7 but occurred from pH 5 to pH 11, which covers the pH range (7--10) measured in CRB groundwaters. The onset of H{sub 2} evolution coincided with the appearance of dissolved Fe{sup 2+}, and an apparent alkaline inhibition could be alleviated by the addition of excess FeCl{sub 2}. This may reflect, in part, the low redox conditions needed for H{sub 2} evolution and suggests that H{sub 2} may be controlled by reaction rates of ferrous silicate minerals. Rates were higher at 60 C than at 30 C, suggesting that the geothermal gradient may lead to increased H{sub 2} production at depth. The results were consistent with the hypothesis at depth. The results were consistent with the hypothesis that basalt-redox reactions support primary production by microorganisms in some terrestrial subsurface environments.

  17. Phytoplankton dynamics of a subtropical reservoir controlled by the complex interplay among hydrological, abiotic, and biotic variables.

    PubMed

    Kuo, Yi-Ming; Wu, Jiunn-Tzong

    2016-12-01

    This study was conducted to identify the key factors related to the spatiotemporal variations in phytoplankton abundance in a subtropical reservoir from 2006 to 2010 and to assist in developing strategies for water quality management. Dynamic factor analysis (DFA), a dimension-reduction technique, was used to identify interactions between explanatory variables (i.e., environmental variables) and abundance (biovolume) of predominant phytoplankton classes. The optimal DFA model significantly described the dynamic changes in abundances of predominant phytoplankton groups (including dinoflagellates, diatoms, and green algae) at five monitoring sites. Water temperature, electrical conductivity, water level, nutrients (total phosphorus, NO3-N, and NH3-N), macro-zooplankton, and zooplankton were the key factors affecting the dynamics of aforementioned phytoplankton. Therefore, transformations of nutrients and reactions between water quality variables and aforementioned processes altered by hydrological conditions may also control the abundance dynamics of phytoplankton, which may represent common trends in the DFA model. The meandering shape of Shihmen Reservoir and its surrounding rivers caused a complex interplay between hydrological conditions and abiotic and biotic variables, resulting in phytoplankton abundance that could not be estimated using certain variables. Additional water quality and hydrological variables at surrounding rivers and monitoring plans should be executed a few days before and after reservoir operations and heavy storm, which would assist in developing site-specific preventive strategies to control phytoplankton abundance.

  18. The effect of abiotic factors on the toxicity of cypermethrin against the snail Lymnaea acuminata in the control of fascioliasis.

    PubMed

    Singh, V; Singh, D K

    2009-03-01

    Every month during the year 2006-2007, the 24, 48, 72 and 96 h LC50 values of a molluscicide, cypermethrin, were determined for a snail Lymnaea acuminata, with concomitant estimation of levels of temperature, pH, dissolved oxygen and carbon dioxide and electrical conductivity, both in control and test water. On the basis of a 24 h toxicity assay, it was noted that LC50 values of 10.39, 10.90 and 11.19 mg l- 1 during the months of May, June and July, respectively, were most effective in killing the snails, while the molluscicide was least effective in the month of January, when its 24 h LC50 was 65.84 mg l- 1.There was a significant positive correlation between LC50 of cypermethrin and levels of dissolved O2/pH of water in corresponding months. On the contrary, a negative correlation was observed between LC50 and dissolved CO2/temperature of test water in the same months. In order to ascertain that such a relationship between toxicity and abiotic factors is not coincidental, the nervous tissue of the snail was assayed for the activity of acetylcholinesterase (AChE), acid phosphatase (ACP) and alkaline phosphatase (ALP) to sublethal concentrations (40% and 80%) of 24 h LC50 during each of the 12 months of the same year. The findings confirmed that abiotic factors indeed influence toxicity of cypermethrin in the snail. A significant positive rank correlation between AChE, ACP and ALP activity did exist following exposure to the corresponding sublethal concentrations. Moreover, there was a maximum inhibition of 61.29 and 76.16% of AChE and ACP, respectively, in snails exposed to 80% of the 24 h LC50 in the month of May. A similar treatment caused a maximum inhibition of 70.53% of ALP activity in the month of June. This work shows conclusively that the best time to control the snail population with cypermethrin is during the months of May and June.

  19. The relationships among biotic and abiotic factors as control soil degradation processes along a Mediterranean pluviometric gradient.

    NASA Astrophysics Data System (ADS)

    Damian Ruiz Sinoga, Jose; Romero Diaz, Asunción

    2010-05-01

    The shifts to soil properties that resulted from changes in water availability along a pluviometric gradient from humid to semiarid/arid Mediterranean climate were analyzed. The study was carried out at eight experimental sites in southern Spain and assessed the influence of a reduction in annual average rainfall along the pluviometric gradient on various physical, chemical and hydrological properties of the topsoil, and on the biotic characteristics of the environment. The aims were to assess the soil degradation status, and to determine if any of the soil variables studied could be used as soil degradation indicators. For each experimental site the relationships among a series of edaphic, hydrological and biological properties were investigated, and the principal factors affecting soil degradation were determined using principal component analysis. The properties included the clay, silt and sand content; organic matter; retained organic carbon; salinity; cation exchange capacity; structural stability; USLE K factor; bulk density; saturated hydraulic conductivity; soil moisture; the number of vegetal species; and vegetation cover. The results showed that relationships between biotic and abiotic factors controlled the soil degradation status along a pluviometric gradient from wet to semiarid/arid conditions in the Mediterranean area, and therefore the stability of the eco-geomorphological system depends on the dominant factor. A precipitation-based soil degradation threshold of approximately 500 mm/yr was established, as below this level the vegetation is no longer associated with the presence of greater soil moisture content, but adapts to degradation, as evidenced by the appearance of xerophytic species.

  20. Biotic, abiotic and management controls on methanol fluxes above a temperate mountain grassland

    NASA Astrophysics Data System (ADS)

    Hörtnagl, Lukas; Bamberger, Ines; Graus, Martin; Ruuskanen, Taina; Schnitzhofer, Ralf; Müller, Markus; Hansel, Armin; Wohlfahrt, Georg

    2010-05-01

    -hourly averages. As methanol is produced in expanding cell walls, the change in the measured green area index (?GAI) was used as a proxy for plant growth. However ?GAI was poorly correlated with methanol fluxes, possible explanations will be discussed. References: Davison, B., Brunner, A., Amman, C., Spirig, C., Jocher, M., Neftel, A. Cut-induced VOC emissions from agricultural grasslands. Plant Biol. 10, 76-85, 2008. Harley, P., Greenberg, J., Niinemets, Ü., and Guenther, A..: Environmental controls over methanol emission from leaves. Biogeosciences, 4, 1083-1099, 2007. Hüve, K., Christ, M., Kleist, E., Uerlings, R., Niinemets, Ü., Walter, A. and Wildt, J.: Simultaneous growth and emission measurements demonstrate an interactive control of methanol release by leaf expansion and stomata. doi:10.1093/jxb/erm038, Journal of Experimental Botany, 2007. Niinemets, Ü. and Reichstein, M.: Controls on the emission of plant volatiles through stomata: A sensitivity analysis. J. Geophys. Res., 108, 4211, doi:10.1029/2002JD002626, 2003. Niinemets, Ü., Loreto, F. and Reichstein, M.: Physiological and physicochemical controls on foliar volatile organic compound emissions. Trends in Plant Science,9, 2004. Wohlfahrt G., Haslwanter A., Hörtnagl L., Jasoni R.L., Fenstermaker L.F., Arnone J.A. III, Hammerle A. (2009) On the consequences of the energy imbalance for calculating surface conductance to water vapour. Agricultural and Forest Meteorology 149, 15561559.

  1. Abiotic & biotic responses of the Colorado River to controlled floods at Glen Canyon Dam, Arizona, USA

    USGS Publications Warehouse

    Korman, Josh; Melis, Ted; Kennedy, Theodore A.

    2012-01-01

    Closure of Glen Canyon Dam reduced sand supply to the Colorado River in Grand Canyon National Park by about 94% while its operation has also eroded the park's sandbar habitats. Three controlled floods released from the dam since 1995 suggest that sandbars might be rebuilt and maintained, but only if repeated floods are timed to follow tributary sand deliveries below the dam. Monitoring data show that sandbars are dynamic and that their erosion after bar building is positively related with mean daily discharge and negatively related with tributary sand production after controlled floods. The March 2008 flood affected non-native rainbow trout abundance in the Lees Ferry tailwater, which supports a blue ribbon fishery. Downstream trout dispersal from the tailwater results in negative competitive interactions and predation on endangered humpback chub. Early survival rates of age-0 trout increased more than fourfold following the 2008 flood, and twofold in 2009, relative to prior years (2006-2007). Hatch-date analysis indicated that early survival rates were much higher for cohorts that emerged about 2 months after the 2008 flood relative to cohorts that emerged earlier that year. The 2009 survival data suggest that tailwater habitat improvements persisted for at least a year, but apparently decreased in 2010. Increased early survival rates for trout coincided with the increased availability of higher quality drifting food items after the 2008 flood owing to an increase in midges and black flies, preferred food items of rainbow trout. Repeated floods from the dam might sustainably rebuild and maintain sandbars if released when new tributary sand is available below the tailwater. Spring flooding might also sustain increased trout abundance and benefit the tailwater fishery, but also be a potential risk to humpback chub in Grand Canyon.

  2. Biotic and abiotic controls on the distribution of tropical forest aboveground biomass

    NASA Astrophysics Data System (ADS)

    Saatchi, S. S.; Schimel, D.; Keller, M. M.; Chambers, J. Q.; Dubayah, R.; Duffy, P.; Yu, Y.; Robinson, C. M.; Chowdhury, D.; Yang, Y.

    2013-12-01

    AUTHOR: Sassan Saatchi1,2, Yan Yang2, Diya Chowdhury2, Yifan Yu2, Chelsea Robinson2, David Schimel1, Paul Duffy3, Michael Keller4, Ralph Dubayah5, Jeffery Chambers6 1. Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA USA 2. Institute of Environment and Sustainability, University of California, Los Angeles, CA, USA 3. Neptune and Company, Inc. Denver, CO, USA 4. International Institute of Tropical Forestry & International Programs, USDA Forest Service, Campinas, Brazil 5. Department of Geography, University of Maryland, College Park, MD, USA 6. Department of Geography, University of California, Berkeley, CA, USA ABSTRACT BODY: In recent years, climate change policies and scientific research created a widespread interest in quantify the carbon stock and changes of global tropical forests extending from forest patches to national and regional scales. Using a combination of inventory data from field plots and forest structure from spaceborne Lidar data, we examine the main controls on the distribution of tropical forest biomass. Here, we concentrate on environmental and landscape variables (precipitation, temperature, topography, and soil), and biotic variables such as functional traits (density of large trees, and wood specific gravity). The analysis is performed using global bioclimatic variables for precipitation and temperature, SRTM data for topographical variables (elevation and ruggedness), and global harmonized soil data for soil type and texture. For biotic variables, we use the GLAS Lidar data to quantify the distribution of large trees, a combined field and remote sensing data for distribution of tree wood specific gravity. The results show that climate variables such as precipitation of dry season can explain the heterogeneity of forest biomass over the landscape but cannot predict the biomass variability significantly and particularly for high biomass forests. Topography such as elevation and ruggedness along with temperature can

  3. Thermal Control Surfaces Experiment

    NASA Technical Reports Server (NTRS)

    Wilkes, D. R.

    1999-01-01

    This report is the final experiment report for the TCSE and summarizes many years of hardware development and analyses. Also included are analyses presented in a number of TCSE papers that were prepared and given at scientific conferences including three LDEF Post-Retrieval Symposiums.

  4. Automating quantum experiment control

    NASA Astrophysics Data System (ADS)

    Stevens, Kelly E.; Amini, Jason M.; Doret, S. Charles; Mohler, Greg; Volin, Curtis; Harter, Alexa W.

    2017-03-01

    The field of quantum information processing is rapidly advancing. As the control of quantum systems approaches the level needed for useful computation, the physical hardware underlying the quantum systems is becoming increasingly complex. It is already becoming impractical to manually code control for the larger hardware implementations. In this chapter, we will employ an approach to the problem of system control that parallels compiler design for a classical computer. We will start with a candidate quantum computing technology, the surface electrode ion trap, and build a system instruction language which can be generated from a simple machine-independent programming language via compilation. We incorporate compile time generation of ion routing that separates the algorithm description from the physical geometry of the hardware. Extending this approach to automatic routing at run time allows for automated initialization of qubit number and placement and additionally allows for automated recovery after catastrophic events such as qubit loss. To show that these systems can handle real hardware, we present a simple demonstration system that routes two ions around a multi-zone ion trap and handles ion loss and ion placement. While we will mainly use examples from transport-based ion trap quantum computing, many of the issues and solutions are applicable to other architectures.

  5. Authentic Research Experience and “Big Data” Analysis in the Classroom: Maize Response to Abiotic Stress

    PubMed Central

    Makarevitch, Irina; Frechette, Cameo; Wiatros, Natalia

    2015-01-01

    Integration of inquiry-based approaches into curriculum is transforming the way science is taught and studied in undergraduate classrooms. Incorporating quantitative reasoning and mathematical skills into authentic biology undergraduate research projects has been shown to benefit students in developing various skills necessary for future scientists and to attract students to science, technology, engineering, and mathematics disciplines. While large-scale data analysis became an essential part of modern biological research, students have few opportunities to engage in analysis of large biological data sets. RNA-seq analysis, a tool that allows precise measurement of the level of gene expression for all genes in a genome, revolutionized molecular biology and provides ample opportunities for engaging students in authentic research. We developed, implemented, and assessed a series of authentic research laboratory exercises incorporating a large data RNA-seq analysis into an introductory undergraduate classroom. Our laboratory series is focused on analyzing gene expression changes in response to abiotic stress in maize seedlings; however, it could be easily adapted to the analysis of any other biological system with available RNA-seq data. Objective and subjective assessment of student learning demonstrated gains in understanding important biological concepts and in skills related to the process of science. PMID:26163561

  6. Effects of biotic and abiotic indices on long term soil moisture data in a grassland biodiversity experiment

    NASA Astrophysics Data System (ADS)

    Fischer, Christine; Hohenbrink, Tobias; Leimer, Sophia; Roscher, Christiane; Ravenek, Janneke; de Kroon, Hans; Kreutziger, Yvonne; Wirth, Christian; Eisenhauer, Nico; Gleixner, Gerd; Weigelt, Alexandra; Mommer, Liesje; Beßler, Holger; Schröder, Boris; Hildebrandt, Anke

    2015-04-01

    small herbs led to higher than average soil moisture in some years (2008, 2012, 2013). Interestingly, plant species richness led to moister than average subsoil at the beginning of the experiment (2003 and 2004), which changed to lower than average up to the year 2010 in all depths. There was no effect of species diversity in the years since 2010, although species diversity generally increases leaf area index and aboveground biomass. The first component from the PCA analysis described the mean behavior in time of all soil moisture time series. The second component reflected the impact of soil depth. The first two components explained 76% of the data set total variance. The third component is linked to plant species richness and explained about 4 % of the total variance of soil moisture data. The fourth component, which explained 2.4 %, showed a high correlation to soil texture. Within this study we investigate the dominant factors controlling spatio-temporal patterns of soil moisture at several soil depths. Although climate and soil depths were the most important drivers, other factors like plant species richness and soil texture affected the temporal variation while certain plant functional groups were important for the spatial variability.

  7. Characterization of tomato Cycling Dof Factors reveals conserved and new functions in the control of flowering time and abiotic stress responses.

    PubMed

    Corrales, Alba-Rocío; Nebauer, Sergio G; Carrillo, Laura; Fernández-Nohales, Pedro; Marqués, Jorge; Renau-Morata, Begoña; Granell, Antonio; Pollmann, Stephan; Vicente-Carbajosa, Jesús; Molina, Rosa-Victoria; Medina, Joaquín

    2014-03-01

    DNA binding with One Finger (DOF) transcription factors are involved in multiple aspects of plant growth and development but their precise roles in abiotic stress tolerance are largely unknown. Here we report a group of five tomato DOF genes, homologous to Arabidopsis Cycling DOF Factors (CDFs), that function as transcriptional regulators involved in responses to drought and salt stress and flowering-time control in a gene-specific manner. SlCDF1-5 are nuclear proteins that display specific binding with different affinities to canonical DNA target sequences and present diverse transcriptional activation capacities in vivo. SlCDF1-5 genes exhibited distinct diurnal expression patterns and were differentially induced in response to osmotic, salt, heat, and low-temperature stresses. Arabidopsis plants overexpressing SlCDF1 or SlCDF3 showed increased drought and salt tolerance. In addition, the expression of various stress-responsive genes, such as COR15, RD29A, and RD10, were differentially activated in the overexpressing lines. Interestingly, overexpression in Arabidopsis of SlCDF3 but not SlCDF1 promotes late flowering through modulation of the expression of flowering control genes such as CO and FT. Overall, our data connect SlCDFs to undescribed functions related to abiotic stress tolerance and flowering time through the regulation of specific target genes and an increase in particular metabolites.

  8. Interactive effects of xenobiotic, abiotic and biotic stressors on Daphnia pulex--results from a multiple stressor experiment with a fractional multifactorial design.

    PubMed

    Scherer, Christian; Seeland, Anne; Oehlmann, Jörg; Müller, Ruth

    2013-08-15

    Pollutant effects on aquatic key species are confounded by multiple abiotic and biotic stressors. To better discriminate and understand the intrinsic and environmental correlates of changing aquatic ecosystems, we untangle in present study how the effects of a low-dosed fungicide on daphnids (via different exposure routes) becomes modified by increasing temperature and the presence of a predator. Using a fractional multifactorial test design, the individual growth, reproduction and population growth rate of Daphnia pulex were investigated under exposure to the fungicide pyrimethanil at an environmental relevant concentration--either directly (via the water phase), indirectly (via food), dually (via water and food) or for multiple generations (fungicide treated source population)--at three temperatures and in presence/absence of the predator kairomones of Chaoborus flavicans. Our results clearly illustrate that multiple stress factors can modify the response of an aquatic key species to pollutants. The environmentally relevant exposure of the contaminant via food or the medium is of same importance. Nevertheless, temperature and the presence of a predator are the dominant factors controlling the reproduction of D. pulex. We conclude that sublethal pyrimethanil pollution can disturb the zooplankton community at suboptimal temperature conditions, but the effects will become masked by low temperatures or if chaoborid larvae are present.

  9. Diagnosing Abiotic Degradation

    EPA Science Inventory

    The abiotic degradation of chlorinated solvents in ground water can be difficult to diagnose. Under current practice, most of the “evidence” is negative; specifically the apparent disappearance of chlorinated solvents with an accumulation of vinyl chloride, ethane, ethylene, or ...

  10. Higher order Arabidopsis 14-3-3 mutants show 14-3-3 involvement in primary root growth both under control and abiotic stress conditions

    PubMed Central

    van Kleeff, P. J. M.; Jaspert, N.; Li, K. W.; Rauch, S.; Oecking, C.; de Boer, A. H.

    2014-01-01

    Arabidopsis 14-3-3 proteins are a family of conserved proteins that interact with numerous partner proteins in a phospho-specific manner, and can affect the target proteins in a number of ways; e.g. modification of enzymatic activity. We isolated T-DNA insertion lines in six 14-3-3 genes within the non-epsilon group that phylogenetically group in three closely related gene pairs. In total, 6 single, 3 double, 12 triple, and 3 quadruple mutants were generated. The mutants were phenotyped for primary root growth on control plates: single and double mutants were indistinguishable from WT, whereas six triples and all quadruples showed a shorter primary root. In addition, length of the first epidermal cell with a visible root hair bulge (LEH) was used to determine primary root elongation on medium containing mannitol and 1-aminocyclopropane-1-carboxylic acid (ACC). This analysis showed clear differences depending on the stress and 14-3-3 gene combinations. Next to the phenotypic growth analyses, a 14-3-3 pull-down assay on roots treated with and without mannitol showed that mannitol stress strongly affects the 14-3-3 interactome. In conclusion, we show gene specificity and functional redundancy among 14-3-3 proteins in primary root elongation under control and under abiotic stress conditions and changes in the 14-3-3 interactome during the onset of stress adaptation. PMID:25189593

  11. Abiotic Versus Biotic Weathering Of Olivine As Possible Biosignatures

    NASA Technical Reports Server (NTRS)

    Longazo, Teresa G.; Wentworth, Susan J.; Clemett, Simon J.; Southam, Gordon; McKay, David S.

    2001-01-01

    We are investigating the weathering of silicate minerals by both purely inorganic, and biologically mediated processes using field-emission scanning electron microscopy (FESEM) and energy dispersive x-ray spectroscopy (EDS). By resolving surface textures and chemical compositions of weathered surfaces at the sub-micron scale we hope to be able to distinguish abiotic from biotic weathering processes and so establish a new biosignature applicable to the study of astromaterials including but not limited to the Martian meteorites. Sterilized olivine grains (San Carlos, Arizona) no more than 1-2 mm in their longest dimension were optically assayed to be uniform in color and free of inclusions were selected as weathering subjects. Prior to all experiments surface morphologies and Fe/Mg ratios were determined for each grain using FE-SEM and EDS. Experiments were divided into two categories abiotic and biotic and were compared with "naturally" weathered samples. For the preliminary experiments, two trials (open and closed to the ambient laboratory environment) were performed under abiotic conditions, and three trials under biotic conditions (control, day 1 and day 2). The open system abiotic trials used sterile grains heated at 98 C and 200 C for both 24 and 48 hours in 1L double distilled de-ionized water. The closed system abiotic trials were conducted under the same conditions but in a sealed two layer steel/Teflon "bomb" apparatus. The biotic trials used sterile grains mounted in a flow-through device attached to a wellhead on the Columbia River aquifer. Several discolored, altered, grains were selected to document "natural" weathering surface textures for comparison with the experimental samples. Preliminary results indicate there are qualitative differences in weathered surface textures among all the designed experiments. The olivine grains in abiotic trials displayed etching, pitting, denticulate margins, dissolution and clay formation. The scale of the features

  12. Oxygen and sulfur isotope systematics of sulfate produced by bacterial and abiotic oxidation of pyrite

    USGS Publications Warehouse

    Balci, N.; Shanks, Wayne C.; Mayer, B.; Mandernack, K.W.

    2007-01-01

    To better understand reaction pathways of pyrite oxidation and biogeochemical controls on ??18O and ??34S values of the generated sulfate in acid mine drainage (AMD) and other natural environments, we conducted a series of pyrite oxidation experiments in the laboratory. Our biological and abiotic experiments were conducted under aerobic conditions by using O2 as an oxidizing agent and under anaerobic conditions by using dissolved Fe(III)aq as an oxidant with varying ??18OH2O values in the presence and absence of Acidithiobacillus ferrooxidans. In addition, aerobic biological experiments were designed as short- and long-term experiments where the final pH was controlled at ???2.7 and 2.2, respectively. Due to the slower kinetics of abiotic sulfide oxidation, the aerobic abiotic experiments were only conducted as long term with a final pH of ???2.7. The ??34SSO4 values from both the biological and abiotic anaerobic experiments indicated a small but significant sulfur isotope fractionation (???-0.7???) in contrast to no significant fractionation observed from any of the aerobic experiments. Relative percentages of the incorporation of water-derived oxygen and dissolved oxygen (O2) to sulfate were estimated, in addition to the oxygen isotope fractionation between sulfate and water, and dissolved oxygen. As expected, during the biological and abiotic anaerobic experiments all of the sulfate oxygen was derived from water. The percentage incorporation of water-derived oxygen into sulfate during the oxidation experiments by O2 varied with longer incubation and lower pH, but not due to the presence or absence of bacteria. These percentages were estimated as 85%, 92% and 87% from the short-term biological, long-term biological and abiotic control experiments, respectively. An oxygen isotope fractionation effect between sulfate and water (??18 OSO4 s(-) H2 O) of ???3.5??? was determined for the anaerobic (biological and abiotic) experiments. This measured ??18 OSO42 - s(-) H2

  13. Abiotic origin of biopolymers

    NASA Technical Reports Server (NTRS)

    Oro, J.; Stephen-Sherwood, E.

    1976-01-01

    A variety of methods have been investigated in different laboratories for the polymerization of amino acids and nucleotides under abiotic conditions. They include (1) thermal polymerization; (2) direct polymerization of certain amino acid nitriles, amides, or esters; (3) polymerization using polyphosphate esters; (4) polymerization under aqueous or drying conditions at moderate temperatures using a variety of simple catalysts or condensing agents like cyanamide, dicyandiamide, or imidazole; and (5) polymerization under similar mild conditions but employing activated monomers or abiotically synthesized high-energy compounds such as adenosine 5'-triphosphate (ATP). The role and significance of these methods for the synthesis of oligopeptides and oligonucleotides under possible primitive-earth conditions is evaluated. It is concluded that the more recent approach involving chemical processes similar to those used by contemporary living organisms appears to offer a reasonable solution to the prebiotic synthesis of these biopolymers.

  14. Real time digital control and controlled structures experiments

    NASA Technical Reports Server (NTRS)

    Rossi, Michael J.; Knowles, Gareth J.; Rauch, Frank

    1991-01-01

    Viewgraphs covering the following topics are given: controlled structures technology at Grumman Corporate Research Center, active and passive control technology, experiment plans, and vacuum chamber test experiment objectives and setup.

  15. Biotic vs. Abiotic Control of Decomposition: A Comparison of the Effects of Simulated Extinctions and Changes in Temperature

    PubMed Central

    Boyero, Luz; Cardinale, Bradley J.; Bastian, Mikis; Pearson, Richard G.

    2014-01-01

    The loss of species is known to have significant effects on ecosystem functioning, but only recently has it been recognized that species loss might rival the effects of other forms of environmental change on ecosystem processes. There is a need for experimental studies that explicitly manipulate species richness and environmental factors concurrently to determine their relative impacts on key ecosystem processes such as plant litter decomposition. It is crucial to understand what factors affect the rate of plant litter decomposition and the relative magnitude of such effects because the rate at which plant litter is lost and transformed to other forms of organic and inorganic carbon determines the capacity for carbon storage in ecosystems and the rate at which greenhouse gasses such as carbon dioxide are outgassed. Here we compared how an increase in water temperature of 5°C and loss of detritivorous invertebrate and plant litter species affect decomposition rates in a laboratory experiment simulating stream conditions. Like some prior studies, we found that species identity, rather than species richness per se, is a key driver of decomposition, but additionally we showed that the loss of particular species can equal or exceed temperature change in its impact on decomposition. Our results indicate that the loss of particular species can be as important a driver of decomposition as substantial temperature change, but also that predicting the relative consequences of species loss and other forms of environmental change on decomposition requires knowledge of assemblages and their constituent species' ecology and ecophysiology. PMID:24466351

  16. Biotic vs. abiotic control of decomposition: a comparison of the effects of simulated extinctions and changes in temperature.

    PubMed

    Boyero, Luz; Cardinale, Bradley J; Bastian, Mikis; Pearson, Richard G

    2014-01-01

    The loss of species is known to have significant effects on ecosystem functioning, but only recently has it been recognized that species loss might rival the effects of other forms of environmental change on ecosystem processes. There is a need for experimental studies that explicitly manipulate species richness and environmental factors concurrently to determine their relative impacts on key ecosystem processes such as plant litter decomposition. It is crucial to understand what factors affect the rate of plant litter decomposition and the relative magnitude of such effects because the rate at which plant litter is lost and transformed to other forms of organic and inorganic carbon determines the capacity for carbon storage in ecosystems and the rate at which greenhouse gasses such as carbon dioxide are outgassed. Here we compared how an increase in water temperature of 5°C and loss of detritivorous invertebrate and plant litter species affect decomposition rates in a laboratory experiment simulating stream conditions. Like some prior studies, we found that species identity, rather than species richness per se, is a key driver of decomposition, but additionally we showed that the loss of particular species can equal or exceed temperature change in its impact on decomposition. Our results indicate that the loss of particular species can be as important a driver of decomposition as substantial temperature change, but also that predicting the relative consequences of species loss and other forms of environmental change on decomposition requires knowledge of assemblages and their constituent species' ecology and ecophysiology.

  17. Abiotic control of underwater light in a drinking water reservoir: Photon budget analysis and implications for water quality monitoring

    NASA Astrophysics Data System (ADS)

    Watanabe, Shohei; Laurion, Isabelle; Markager, Stiig; Vincent, Warwick F.

    2015-08-01

    In optically complex inland waters, the underwater attenuation of photosynthetically active radiation (PAR) is controlled by a variable combination of absorption and scattering components of the lake or river water. Here we applied a photon budget approach to identify the main optical components affecting PAR attenuation in Lake St. Charles, a drinking water reservoir for Québec City, Canada. This analysis showed the dominant role of colored dissolved organic matter (CDOM) absorption (average of 44% of total absorption during the sampling period), but with large changes over depth in the absolute and relative contribution of the individual absorption components (water, nonalgal particulates, phytoplankton and CDOM) to PAR attenuation. This pronounced vertical variation occurred because of the large spectral changes in the light field with depth, and it strongly affected the average in situ diffuse absorption coefficients in the water column. For example, the diffuse absorption coefficient for pure-water in the ambient light field was 10-fold higher than the value previously measured in the blue open ocean and erroneously applied to lakes and coastal waters. Photon absorption budget calculations for a range of limnological conditions confirmed that phytoplankton had little direct influence on underwater light, even at chlorophyll a values above those observed during harmful algal blooms in the lake. These results imply that traditional measures of water quality such as Secchi depth and radiometric transparency do not provide a meaningful estimate of the biological state of the water column in CDOM-colored lakes and reservoirs.

  18. Soil abiotic factors influence interactions between belowground herbivores and plant roots.

    PubMed

    Erb, Matthias; Lu, Jing

    2013-03-01

    Root herbivores are important ecosystem drivers and agricultural pests, and, possibly as a consequence, plants protect their roots using a variety of defensive strategies. One aspect that distinguishes belowground from aboveground plant-insect interactions is that roots are constantly exposed to a set of soil-specific abiotic factors. These factors can profoundly influence root resistance, and, consequently, the outcome of the interaction with belowground feeders. In this review, we synthesize the current literature on the impact of soil moisture, nutrients, and texture on root-herbivore interactions. We show that soil abiotic factors influence the interaction by modulating herbivore abundance and behaviour, root growth and resistance, beneficial microorganisms, as well as natural enemies of the herbivores. We suggest that abiotic heterogeneity may explain the high variability that is often encountered in root-herbivore systems. We also propose that under abiotic stress, the relative fitness value of the roots and the potential negative impact of herbivory increases, which may lead to a higher defensive investment and an increased recruitment of beneficial microorganisms by the plant. At the same time, both root-feeding herbivores and natural enemies are likely to decrease in abundance under extreme environmental conditions, leading to a context- and species-specific impact on plant fitness. Only by using tightly controlled experiments that include soil abiotic heterogeneity will it be possible to understand the impact of root feeders on an ecosystem scale and to develop predictive models for pest occurrence and impact.

  19. Field Experiments in Litter Control

    ERIC Educational Resources Information Center

    Finnie, William C.

    1973-01-01

    A series of urban and highway litter experiments in Richmond (Virginia), St. Louis, and Philadelphia indicated well-designed litter cans reduced littering about 15 percent along city streets and nearly 30 percent along highways. Also, the propensity to litter is critically affected by the characteristics of the individual and environmental…

  20. Climate Change: A Controlled Experiment

    SciTech Connect

    Wullschleger, Stan D; Strahl, Maya

    2010-01-01

    Researchers are altering temperature, carbon dioxide and precipitation levels across plots of forests, grasses and crops to see how plant life responds. Warmer temperatures and higher CO{sub 2} concentrations generally result in more leaf growth or crop yield, but these factors can also raise insect infestation and weaken plants ability to ward off pests and disease. Future field experiments that can manipulate all three conditions at once will lead to better models of how long-term climate changes will affect ecosystems worldwide.

  1. Baseline experiments in teleoperator control

    NASA Technical Reports Server (NTRS)

    Hankins, W. W., III; Mixon, R. W.

    1986-01-01

    Studies have been conducted at the NASA Langley Research Center (LaRC) to establish baseline human teleoperator interface data and to assess the influence of some of the interface parameters on human performance in teleoperation. As baseline data, the results will be used to assess future interface improvements resulting from this research in basic teleoperator human factors. In addition, the data have been used to validate LaRC's basic teleoperator hardware setup and to compare initial teleoperator study results. Four subjects controlled a modified industrial manipulator to perform a simple task involving both high and low precision. Two different schemes for controlling the manipulator were studied along with both direct and indirect viewing of the task. Performance of the task was measured as the length of time required to complete the task along with the number of errors made in the process. Analyses of variance were computed to determine the significance of the influences of each of the independent variables. Comparisons were also made between the LaRC data and data taken earlier by Grumman Aerospace Corp. at their facilities.

  2. RBM25 Mediates Abiotic Responses in Plants

    PubMed Central

    Cheng, Chunhong; Wang, Zhijuan; Yuan, Bingjian; Li, Xia

    2017-01-01

    Alternative splicing (AS) of pre-mRNAs is one of the most important post-transcriptional regulations that enable a single gene to code for multiple proteins resulting in the biodiversity of proteins in eukaryotes. Recently, we have shown that an Arabidopsis thaliana RNA recognition motif-containing protein RBM25 is a novel splicing factor to modulate plant response to ABA during seed germination and post-germination through regulating HAB1 pre-mRNA AS. Here, we show that RBM25 is preferentially expressed in stomata and vascular tissues in Arabidopsis and is induced by ABA and abiotic stresses. Loss-of-function mutant is highly tolerant to drought and sensitive to salt stress. Bioinformatic analysis and expression assays reveal that Arabidopsis RBM25 is induced by multiple abiotic stresses, suggesting a crucial role of RBM25 in Arabidopsis responses to adverse environmental conditions. Furthermore, we provide a comprehensive characterization of the homologous genes of Arabidopsis RBM25 based on the latest plant genome sequences and public microarray databases. Fourteen homologous genes are identified in different plant species which show similar structure in gene and protein. Notably, the promoter analysis reveals that RBM25 homologs are likely controlled by the regulators involved in multiple plant growth and abiotic stresses, such as drought and unfavorable temperature. The comparative analysis of general and unique cis regulatory elements of the RBM25 homologs highlights the conserved and unique molecular processes that modulate plant response to abiotic stresses through RBM25-mediated alternative splicing. PMID:28344583

  3. RBM25 Mediates Abiotic Responses in Plants.

    PubMed

    Cheng, Chunhong; Wang, Zhijuan; Yuan, Bingjian; Li, Xia

    2017-01-01

    Alternative splicing (AS) of pre-mRNAs is one of the most important post-transcriptional regulations that enable a single gene to code for multiple proteins resulting in the biodiversity of proteins in eukaryotes. Recently, we have shown that an Arabidopsis thaliana RNA recognition motif-containing protein RBM25 is a novel splicing factor to modulate plant response to ABA during seed germination and post-germination through regulating HAB1 pre-mRNA AS. Here, we show that RBM25 is preferentially expressed in stomata and vascular tissues in Arabidopsis and is induced by ABA and abiotic stresses. Loss-of-function mutant is highly tolerant to drought and sensitive to salt stress. Bioinformatic analysis and expression assays reveal that Arabidopsis RBM25 is induced by multiple abiotic stresses, suggesting a crucial role of RBM25 in Arabidopsis responses to adverse environmental conditions. Furthermore, we provide a comprehensive characterization of the homologous genes of Arabidopsis RBM25 based on the latest plant genome sequences and public microarray databases. Fourteen homologous genes are identified in different plant species which show similar structure in gene and protein. Notably, the promoter analysis reveals that RBM25 homologs are likely controlled by the regulators involved in multiple plant growth and abiotic stresses, such as drought and unfavorable temperature. The comparative analysis of general and unique cis regulatory elements of the RBM25 homologs highlights the conserved and unique molecular processes that modulate plant response to abiotic stresses through RBM25-mediated alternative splicing.

  4. Thermal control surfaces experiment flight system performance

    NASA Technical Reports Server (NTRS)

    Wilkes, Donald R.; Hummer, Leigh L.; Zwiener, James M.

    1991-01-01

    The Thermal Control Surfaces Experiment (TCSE) is the most complex system, other than the LDEF, retrieved after long term space exposure. The TCSE is a microcosm of complex electro-optical payloads being developed and flow by NASA and the DoD including SDI. The objective of TCSE was to determine the effects of the near-Earth orbital environment and the LDEF induced environment on spacecraft thermal control surfaces. The TCSE was a comprehensive experiment that combined in-space measurements with extensive post flight analyses of thermal control surfaces to determine the effects of exposure to the low earth orbit space environment. The TCSE was the first space experiment to measure the optical properties of thermal control surfaces the way they are routinely measured in a lab. The performance of the TCSE confirms that low cost, complex experiment packages can be developed that perform well in space.

  5. The relative abundances of resolved l2CH2D2 and 13CH3D and mechanisms controlling isotopic bond ordering in abiotic and biotic methane gases

    NASA Astrophysics Data System (ADS)

    Young, E. D.; Kohl, I. E.; Lollar, B. Sherwood; Etiope, G.; Rumble, D.; Li (李姝宁), S.; Haghnegahdar, M. A.; Schauble, E. A.; McCain, K. A.; Foustoukos, D. I.; Sutclife, C.; Warr, O.; Ballentine, C. J.; Onstott, T. C.; Hosgormez, H.; Neubeck, A.; Marques, J. M.; Pérez-Rodríguez, I.; Rowe, A. R.; LaRowe, D. E.; Magnabosco, C.; Yeung, L. Y.; Ash, J. L.; Bryndzia, L. T.

    2017-04-01

    We report measurements of resolved 12CH2D2 and 13CH3D at natural abundances in a variety of methane gases produced naturally and in the laboratory. The ability to resolve 12CH2D2 from 13CH3D provides unprecedented insights into the origin and evolution of CH4. The results identify conditions under which either isotopic bond order disequilibrium or equilibrium are expected. Where equilibrium obtains, concordant Δ12CH2D2 and Δ13CH3D temperatures can be used reliably for thermometry. We find that concordant temperatures do not always match previous hypotheses based on indirect estimates of temperature of formation nor temperatures derived from CH4/H2 D/H exchange, underscoring the importance of reliable thermometry based on the CH4 molecules themselves. Where Δ12CH2D2 and Δ13CH3D values are inconsistent with thermodynamic equilibrium, temperatures of formation derived from these species are spurious. In such situations, while formation temperatures are unavailable, disequilibrium isotopologue ratios nonetheless provide novel information about the formation mechanism of the gas and the presence or absence of multiple sources or sinks. In particular, disequilibrium isotopologue ratios may provide the means for differentiating between methane produced by abiotic synthesis vs. biological processes. Deficits in 12CH2D2 compared with equilibrium values in CH4 gas made by surface-catalyzed abiotic reactions are so large as to point towards a quantum tunneling origin. Tunneling also accounts for the more moderate depletions in 13CH3D that accompany the low 12CH2D2 abundances produced by abiotic reactions. The tunneling signature may prove to be an important tracer of abiotic methane formation, especially where it is preserved by dissolution of gas in cool hydrothermal systems (e.g., Mars). Isotopologue signatures of abiotic methane production can be erased by infiltration of microbial communities, and Δ12CH2D2 values are a key tracer of microbial recycling.

  6. ASSISTments Dataset from Multiple Randomized Controlled Experiments

    ERIC Educational Resources Information Center

    Selent, Douglas; Patikorn, Thanaporn; Heffernan, Neil

    2016-01-01

    In this paper, we present a dataset consisting of data generated from 22 previously and currently running randomized controlled experiments inside the ASSISTments online learning platform. This dataset provides data mining opportunities for researchers to analyze ASSISTments data in a convenient format across multiple experiments at the same time.…

  7. Experiments with the KITE attitude control simulator

    NASA Technical Reports Server (NTRS)

    Powell, J. David; Kline-Schoder, Robert

    1989-01-01

    Simulation experiments are conducted to test an attitude control technique for tethered satellites using the tether tension force to generate control torques by moving the tether attach point relative to the satellite center of mass. A scaled, one-dimensional, air-bearing supported laboratory simulation of the Kinetic Isolation Tether Experiment shows that the attitude of the simulator can be regulated to within 0.75 arcsec with a bandwidth of about 0.1 Hz. The control design includes a state estimator to calculate the vehicle mass center and to calculate the effect of the stepper motor dynamics on the state estimate. Results are presented from closed-loop attitude control experiments to verify the attitude control technique.

  8. Temperature controls on sediment production in the Oregon Coast Range - abiotic frost-cracking processes vs. biotic-dominated processes over the last 40 ka

    NASA Astrophysics Data System (ADS)

    Marshall, J. A.; Roering, J. J.; Praskievicz, S. J.; Hales, T. C.; Gavin, D. G.; Bartlein, P. J.

    2012-12-01

    The Oregon Coast Range (OCR) is a mid-latitude soil-mantled landscape wherein measured uplift rates are broadly consistent with long-term measured erosion rates. The OCR was unglaciated during the last glacial period (~ 26 to 13 ka) and therefore is considered an ideal steady-state landscape to study and model geomorphic processes. However, previously published paleoclimate data inferred from a 42 ka paleolake fossil archive in the OCR Little Lake watershed (3 km2) strongly suggest that temperatures in the OCR during the last glacial were well within the frost cracking temperature window of -3 to -8 °C. Therefore, we suggest that while present-day OCR sediment production is dominated by biota, specifically trees, frost-driven abiotic processes may have played a significant role in modulating erosion rates and landscape evolution during the last glacial interval. A new sediment core from the Little Lake basin at the lake's edge, centered proximal to hillslopes, spans ~ 50 ka to 20 ka. We observe a fourfold increase in sediment accumulation rates from the non-glacial interval (~50 ka to ~ 26 ka) to the last glacial interval (~ 26 ka to ~ 20 ka), including > 12 m of sediment from the last glacial maximum, dated at 23,062 - 23,581 cal yr B.P. The decreased inferred temperatures and increased sedimentation rates suggest increased sediment production and transport via frost processes during the last glacial interval, in contrast to sediment production and erosion rates controlled by biotic processes in the non-glacial intervals. We present a climate-time series scenario of likely frost-cracking intensity across the entire Oregon Coast Range from the non-glacial interval (at least 3 °C cooler than present-day temperatures) through the glacial interval (7 to 14 °C cooler) and into the Holocene (January temperatures ~ 5 °C). We use the PRISM dataset, which consists of monthly temperature and precipitation for the contiguous United States, to calculate local monthly

  9. Abiotic stresses and endophyte effects

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Abiotic stresses consist of nonorganismal, nonpathogenic factors that inhibit plant function. Tall fescue [Lolium arundinaceum (Schreb.) Darbysh.] is widely symbiotic with a naturally occurring endophytic fungus [Neotyphodium coenophialum (Morgan-Jones and Gams) Glenn, Bacon, and Hanlin], which con...

  10. Abiotic tooth enamel

    NASA Astrophysics Data System (ADS)

    Yeom, Bongjun; Sain, Trisha; Lacevic, Naida; Bukharina, Daria; Cha, Sang-Ho; Waas, Anthony M.; Arruda, Ellen M.; Kotov, Nicholas A.

    2017-03-01

    Tooth enamel comprises parallel microscale and nanoscale ceramic columns or prisms interlaced with a soft protein matrix. This structural motif is unusually consistent across all species from all geological eras. Such invariability—especially when juxtaposed with the diversity of other tissues—suggests the existence of a functional basis. Here we performed ex vivo replication of enamel-inspired columnar nanocomposites by sequential growth of zinc oxide nanowire carpets followed by layer-by-layer deposition of a polymeric matrix around these. We show that the mechanical properties of these nanocomposites, including hardness, are comparable to those of enamel despite the nanocomposites having a smaller hard-phase content. Our abiotic enamels have viscoelastic figures of merit (VFOM) and weight-adjusted VFOM that are similar to, or higher than, those of natural tooth enamels—we achieve values that exceed the traditional materials limits of 0.6 and 0.8, respectively. VFOM values describe resistance to vibrational damage, and our columnar composites demonstrate that light-weight materials of unusually high resistance to structural damage from shocks, environmental vibrations and oscillatory stress can be made using biomimetic design. The previously inaccessible combinations of high stiffness, damping and light weight that we achieve in these layer-by-layer composites are attributed to efficient energy dissipation in the interfacial portion of the organic phase. The in vivo contribution of this interfacial portion to macroscale deformations along the tooth’s normal is maximized when the architecture is columnar, suggesting an evolutionary advantage of the columnar motif in the enamel of living species. We expect our findings to apply to all columnar composites and to lead to the development of high-performance load-bearing materials.

  11. Abiotic tooth enamel.

    PubMed

    Yeom, Bongjun; Sain, Trisha; Lacevic, Naida; Bukharina, Daria; Cha, Sang-Ho; Waas, Anthony M; Arruda, Ellen M; Kotov, Nicholas A

    2017-03-01

    Tooth enamel comprises parallel microscale and nanoscale ceramic columns or prisms interlaced with a soft protein matrix. This structural motif is unusually consistent across all species from all geological eras. Such invariability-especially when juxtaposed with the diversity of other tissues-suggests the existence of a functional basis. Here we performed ex vivo replication of enamel-inspired columnar nanocomposites by sequential growth of zinc oxide nanowire carpets followed by layer-by-layer deposition of a polymeric matrix around these. We show that the mechanical properties of these nanocomposites, including hardness, are comparable to those of enamel despite the nanocomposites having a smaller hard-phase content. Our abiotic enamels have viscoelastic figures of merit (VFOM) and weight-adjusted VFOM that are similar to, or higher than, those of natural tooth enamels-we achieve values that exceed the traditional materials limits of 0.6 and 0.8, respectively. VFOM values describe resistance to vibrational damage, and our columnar composites demonstrate that light-weight materials of unusually high resistance to structural damage from shocks, environmental vibrations and oscillatory stress can be made using biomimetic design. The previously inaccessible combinations of high stiffness, damping and light weight that we achieve in these layer-by-layer composites are attributed to efficient energy dissipation in the interfacial portion of the organic phase. The in vivo contribution of this interfacial portion to macroscale deformations along the tooth's normal is maximized when the architecture is columnar, suggesting an evolutionary advantage of the columnar motif in the enamel of living species. We expect our findings to apply to all columnar composites and to lead to the development of high-performance load-bearing materials.

  12. Abiotic emissions of methane and reduced organic compounds from organic matter

    NASA Astrophysics Data System (ADS)

    Roeckmann, T.; Keppler, F.; Vigano, I.; Derendorp, L.; Holzinger, R.

    2012-12-01

    Recent laboratory studies show that the important greenhouse gas methane, but also other reduced atmospheric trace gases, can be emitted by abiotic processes from organic matter, such as plants, pure organic compounds and soils. It is very difficult to distinguish abiotic from biotic emissions in field studies, but in laboratory experiments this is easier because it is possible to carefully prepare/sterilize samples, or to control external parameters. For example, the abiotic emissions always show a strong increase with temperature when temperatures are increased to 70C or higher, well above the temperature optimum for bacterial activity. UV radiation has also been clearly shown to lead to emission of methane and other reduced gases from organic matter. Interesting information on the production mechanism has been obtained from isotope studies, both at natural abundance and with isotope labeling. For example, the methoxyl groups of pectin were clearly identified to produce methane. However, analysis of the isotopic composition of methane from natural samples clearly indicates that there must be other molecular mechanisms that lead to methane production. Abiotic methane generation could be a ubiquitous process that occurs naturally at low rates from many different sources.

  13. A slewing control experiment for flexible structures

    NASA Technical Reports Server (NTRS)

    Juang, J.-N.; Horta, L. G.; Robertshaw, H. H.

    1985-01-01

    A hardware set-up has been developed to study slewing control for flexible structures including a steel beam and a solar panel. The linear optimal terminal control law is used to design active controllers which are implemented in an analog computer. The objective of this experiment is to demonstrate and verify the dynamics and optimal terminal control laws as applied to flexible structures for large angle maneuver. Actuation is provided by an electric motor while sensing is given by strain gages and angle potentiometer. Experimental measurements are compared with analytical predictions in terms of modal parameters of the system stability matrix and sufficient agreement is achieved to validate the theory.

  14. Wavefront Control Testbed (WCT) Experiment Results

    NASA Technical Reports Server (NTRS)

    Burns, Laura A.; Basinger, Scott A.; Campion, Scott D.; Faust, Jessica A.; Feinberg, Lee D.; Hayden, William L.; Lowman, Andrew E.; Ohara, Catherine M.; Petrone, Peter P., III

    2004-01-01

    The Wavefront Control Testbed (WCT) was created to develop and test wavefront sensing and control algorithms and software for the segmented James Webb Space Telescope (JWST). Last year, we changed the system configuration from three sparse aperture segments to a filled aperture with three pie shaped segments. With this upgrade we have performed experiments on fine phasing with line-of-sight and segment-to-segment jitter, dispersed fringe visibility and grism angle;. high dynamic range tilt sensing; coarse phasing with large aberrations, and sampled sub-aperture testing. This paper reviews the results of these experiments.

  15. Modular control system for optogenetic experiments

    NASA Astrophysics Data System (ADS)

    Sowiński, Mikołaj; Kulik, Paweł; Kasprowicz, Grzegorz; Mankiewicz, Lech; Krawczyk, Rafał D.; Jarosiński, Jakub; Czajkowski, Rafał; Knapska, Ewelina; Puścian, Alicja; Kowalski, Jakub; Rusakov, Konstantin; Przywózki, Tomasz; Rasiński, Paweł; Juszczyk, Bartłomiej

    2016-09-01

    This article presents a modular control system used in Eco-HAB experimentation system. Features specific to the solution are covered. Control system is described in details. The architecture is outlined in the context of requirements to be met. Modes of utilization of implantable device, time synchronization, localization service and antenna driving oscillation fine-tuning as well as preliminary experiments in preparation are described.

  16. Diffraction experiments with infrared remote controls

    NASA Astrophysics Data System (ADS)

    Kuhn, Jochen; Vogt, Patrik

    2012-02-01

    In this paper we describe an experiment in which radiation emitted by an infrared remote control is passed through a diffraction grating. An image of the diffraction pattern is captured using a cell phone camera and then used to determine the wavelength of the radiation.

  17. Distributed control network for optogenetic experiments

    NASA Astrophysics Data System (ADS)

    Kasprowicz, G.; Juszczyk, B.; Mankiewicz, L.

    2014-11-01

    Nowadays optogenetic experiments are constructed to examine social behavioural relations in groups of animals. A novel concept of implantable device with distributed control network and advanced positioning capabilities is proposed. It is based on wireless energy transfer technology, micro-power radio interface and advanced signal processing.

  18. Circadian regulation of abiotic stress tolerance in plants

    PubMed Central

    Grundy, Jack; Stoker, Claire; Carré, Isabelle A.

    2015-01-01

    Extremes of temperatures, drought and salinity cause widespread crop losses throughout the world and impose severe limitations on the amount of land that can be used for agricultural purposes. Hence, there is an urgent need to develop crops that perform better under such abiotic stress conditions. Here, we discuss intriguing, recent evidence that circadian clock contributes to plants’ ability to tolerate different types of environmental stress, and to acclimate to them. The clock controls expression of a large fraction of abiotic stress-responsive genes, as well as biosynthesis and signaling downstream of stress response hormones. Conversely, abiotic stress results in altered expression and differential splicing of the clock genes, leading to altered oscillations of downstream stress-response pathways. We propose a range of mechanisms by which this intimate coupling between the circadian clock and environmental stress-response pathways may contribute to plant growth and survival under abiotic stress. PMID:26379680

  19. Decentralized control experiments on NASA's flexible grid

    NASA Technical Reports Server (NTRS)

    Ozguner, U.; Yurkowich, S.; Martin, J., III; Al-Abbass, F.

    1986-01-01

    Methods arising from the area of decentralized control are emerging for analysis and control synthesis for large flexible structures. In this paper the control strategy involves a decentralized model reference adaptive approach using a variable structure control. Local models are formulated based on desired damping and response time in a model-following scheme for various modal configurations. Variable structure controllers are then designed employing co-located angular rate and position feedback. In this scheme local control forces the system to move on a local sliding mode in some local error space. An important feature of this approach is that the local subsystem is made insensitive to dynamical interactions with other subsystems once the sliding surface is reached. Experiments based on the above have been performed for NASA's flexible grid experimental apparatus. The grid is designed to admit appreciable low-frequency structural dynamics, and allows for implementation of distributed computing components, inertial sensors, and actuation devices. A finite-element analysis of the grid provides the model for control system design and simulation; results of several simulations are reported on here, and a discussion of application experiments on the apparatus is presented.

  20. Middeck Active Control Experiment (MACE), phase A

    NASA Technical Reports Server (NTRS)

    Crawley, Edward F.; Deluis, Javier; Miller, David W.

    1989-01-01

    A rationale to determine which structural experiments are sufficient to verify the design of structures employing Controlled Structures Technology was derived. A survey of proposed NASA missions was undertaken to identify candidate test articles for use in the Middeck Active Control Experiment (MACE). The survey revealed that potential test articles could be classified into one of three roles: development, demonstration, and qualification, depending on the maturity of the technology and the mission the structure must fulfill. A set of criteria was derived that allowed determination of which role a potential test article must fulfill. A review of the capabilities and limitations of the STS middeck was conducted. A reference design for the MACE test article was presented. Computing requirements for running typical closed-loop controllers was determined, and various computer configurations were studied. The various components required to manufacture the structure were identified. A management plan was established for the remainder of the program experiment development, flight and ground systems development, and integration to the carrier. Procedures for configuration control, fiscal control, and safety, reliabilty, and quality assurance were developed.

  1. Progress on control experiments of flexible structures

    NASA Technical Reports Server (NTRS)

    Juang, Jer-Nan

    1990-01-01

    Progress at the NASA Langley Research Center in the area of control experiments for flexible structures is described. First the author presents the experimental results for a linear model which represents slewing maneuvers of a generic space station solar panel carried out to evaluate experimentally some control technologies. Then the status of the rotational/translational maneuvering experiment of a flexible steel panel carried by a translation cart is presented. Finally, experimental results of the NASA minimast testbed using velocity command stepper motors as reaction mass reactors are shown. All the test configurations are briefly described, including actuator and sensor, test setup, and test software. The status of some research activities oriented primarily to the experimental methods for control of flexible structures is presented.

  2. The Middeck Active Control Experiment (MACE)

    NASA Technical Reports Server (NTRS)

    Miller, David W.; Sepe, Raymond B.; Rey, Daniel; Saarmaa, Erik; Crawley, Edward F.

    1993-01-01

    The Middeck Active Control Experiment (MACE) is a NASA In-Step and Control Structure Interaction (CSI) Office funded Shuttle middeck experiment. The objective is to investigate the extent to which closed-loop behavior of flexible spacecraft in zero-gravity (0-g) can be predicted. This prediction becomes particularly difficult when dynamic behavior during ground testing exhibits extensive suspension and direct gravity coupling. On-orbit system identification and control reconfiguration is investigated to improve performance which would otherwise be limited due to errors in prediction. The program is presently in its preliminary design phase with launch expected in the summer of 1994. The MACE test article consists of three attitude control torque wheels, a two axis gimballing payload, inertial sensors and a flexible support structure. With the acquisition of a second payload, this will represent a multiple payload platform with significant structural flexibility. This paper presents on-going work in the areas of modelling and control of the MACE test article in the zero and one-gravity environments. Finite element models, which include suspension and gravity effects, and measurement models, derived from experimental data, are used as the basis for Linear Quadratic Gaussian controller designs. Finite element based controllers are analytically used to study the differences in closed-loop performance as the test article transitions between the 0-g and 1-g environments. Measurement based controllers are experimentally applied to the MACE test article in the 1-g environment and achieve over an order of magnitude improvement in payload pointing accuracy when disturbed by a broadband torque disturbance. The various aspects of the flight portion of the experiment are also discussed.

  3. An omics approach to understand the plant abiotic stress.

    PubMed

    Debnath, Mousumi; Pandey, Mukeshwar; Bisen, P S

    2011-11-01

    Abiotic stress can lead to changes in development, productivity, and severe stress and may even threaten survival of plants. Several environmental stresses cause drastic changes in the growth, physiology, and metabolism of plants leading to the increased accumulation of secondary metabolites. As medicinal plants are important sources of drugs, steps are taken to understand the effect of stress on the physiology, biochemistry, genomic, proteomic, and metabolic levels. The molecular responses of plants to abiotic stress are often considered as a complex process. They are mainly based on the modulation of transcriptional activity of stress-related genes. Many genes have been induced under stress conditions. The products of stress-inducible genes protecting against these stresses includes the enzymes responsible for the synthesis of various osmoprotectants. Genetic engineering of tolerance to abiotic stresses help in molecular understanding of pathways induced in response to one or more of the abiotic stresses. Systems biology and virtual experiments allow visualizing and understanding how plants work to overcome abiotic stress. This review discusses the omic approach to understand the plant response to abiotic stress with special emphasis on medicinal plant.

  4. APECS - The Atacama Pathfinder Experiment Control System

    NASA Astrophysics Data System (ADS)

    Muders, D.; Hafok, H.; Wyrowski, F.; Polehampton, E.; Belloche, A.; König, C.; Schaaf, R.

    2006-07-01

    APECS is the CORBA based, distributed control system for the new Atacama Pathfinder EXperiment (APEX) Telescope located at the Llano de Chajnantor at an altitude of 5100m in the Atacama desert in northern Chile. The telescope has been operational since August 2005 and APECS is now being used for regular science observations. APECS employs a modern, object-oriented design. Generic interfaces facilitate adding new instruments. The IPython based observer command language allows using macros and creating more complex observing modes.

  5. A Controlled Shear Decorrelation Experiment (CSDX)

    NASA Astrophysics Data System (ADS)

    Tynan, George

    1999-11-01

    The controlled shear de-correlation experiment (CSDX) is being designed to study the effect of sheared flows on: (1) known linear pressure gradient-driven drift and/or effective gravity-driven flute eigenmodes; (2) the nonlinear three-wave coupling of a finite number of large amplitude coherent modes; and (3) on the rate of electrostatic turbulent fluctuation energy cascades. This research is motivated by magnetic confinement experiments and theory which suggest that sheared E x B shear flows lead to a nonlinear de-correlation of plasma turbulence. The plasma state (i.e. quiescent, single small-amplitude drift wave, nonlinearly coupled modes, or strongly turbulent) will be controlled by varying the magnetic field strength, collisionality, parallel current, and/or effective gravity due to solid-body plasma rotation driven by azimuthal ExB drifts. The radial electric field strength and shear rate will be controlled independently of the plasma state by the application of externally controlled voltages to concentric annular rings which will form the ends of the experimental region. Diagnostics include azimuthal, radial, and axial Langmuir probe arrays, laser induced flourescence (LIF) for ion temperature and equilibrium ExB flow velocities. Bi-spectral analysis techniques will be used to measure k-space resolved linear growth rates and nonlinear energy cascading due to three-wave coupling in the presence of flow shear. Designs and experimental plans will be presented and discussed.

  6. Basic radiological studies contamination control experiments

    SciTech Connect

    Duce, S.W.; Winberg, M.R.; Freeman, A.L.

    1989-09-01

    This report describes the results of experiments relating to contamination control performed in support of the Environmental Restoration Programs Retrieval Project. During the years 1950 to 1970 waste contaminated with plutonium and other transuranic radionuclides was disposed of in shallow land-filled pits and trenches at the Idaho National Engineering Laboratory. Due to potential for migration of radionuclides to an existing aquifer the feasibility of retrieving and repackaging the waste for placement in a final repository is being examined as part of a retrieval project. Contamination control experiments were conducted to determine expected respirable and nonrespirable plutonium contaminated dust fractions and the effectiveness of various dust suppression techniques. Three soil types were tested to determine respirable fractions: Rocky Flats Plant generic soil, Radioactive Waste Management Complex generic soil, and a 1:1 blend of the two soil types. Overall, the average respirable fraction of airborne dust was 5.4% by weight. Three contamination control techniques were studied: soil fixative sprays, misting agents, and dust suppression agents. All of the tested agents proved to be effective in reducing dust in the air. Details of product performance and recommended usage are discussed.

  7. Abiotic methane formation during experimental serpentinization of olivine.

    PubMed

    McCollom, Thomas M

    2016-12-06

    Fluids circulating through actively serpentinizing systems are often highly enriched in methane (CH4). In many cases, the CH4 in these fluids is thought to derive from abiotic reduction of inorganic carbon, but the conditions under which this process can occur in natural systems remain unclear. In recent years, several studies have reported abiotic formation of CH4 during experimental serpentinization of olivine at temperatures at or below 200 °C. However, these results seem to contradict studies conducted at higher temperatures (300 °C to 400 °C), where substantial kinetic barriers to CH4 synthesis have been observed. Here, the potential for abiotic formation of CH4 from dissolved inorganic carbon during olivine serpentinization is reevaluated in a series of laboratory experiments conducted at 200 °C to 320 °C. A (13)C-labeled inorganic carbon source was used to unambiguously determine the origin of CH4 generated in the experiments. Consistent with previous high-temperature studies, the results indicate that abiotic formation of CH4 from reduction of dissolved inorganic carbon during the experiments is extremely limited, with nearly all of the observed CH4 derived from background sources. The results indicate that the potential for abiotic synthesis of CH4 in low-temperature serpentinizing environments may be much more limited than some recent studies have suggested. However, more extensive production of CH4 was observed in one experiment performed under conditions that allowed an H2-rich vapor phase to form, suggesting that shallow serpentinization environments where a separate gas phase is present may be more favorable for abiotic synthesis of CH4.

  8. Abiotic methane formation during experimental serpentinization of olivine

    NASA Astrophysics Data System (ADS)

    McCollom, Thomas M.

    2016-12-01

    Fluids circulating through actively serpentinizing systems are often highly enriched in methane (CH4). In many cases, the CH4 in these fluids is thought to derive from abiotic reduction of inorganic carbon, but the conditions under which this process can occur in natural systems remain unclear. In recent years, several studies have reported abiotic formation of CH4 during experimental serpentinization of olivine at temperatures at or below 200 °C. However, these results seem to contradict studies conducted at higher temperatures (300 °C to 400 °C), where substantial kinetic barriers to CH4 synthesis have been observed. Here, the potential for abiotic formation of CH4 from dissolved inorganic carbon during olivine serpentinization is reevaluated in a series of laboratory experiments conducted at 200 °C to 320 °C. A 13C-labeled inorganic carbon source was used to unambiguously determine the origin of CH4 generated in the experiments. Consistent with previous high-temperature studies, the results indicate that abiotic formation of CH4 from reduction of dissolved inorganic carbon during the experiments is extremely limited, with nearly all of the observed CH4 derived from background sources. The results indicate that the potential for abiotic synthesis of CH4 in low-temperature serpentinizing environments may be much more limited than some recent studies have suggested. However, more extensive production of CH4 was observed in one experiment performed under conditions that allowed an H2-rich vapor phase to form, suggesting that shallow serpentinization environments where a separate gas phase is present may be more favorable for abiotic synthesis of CH4.

  9. APECS - the Atacama pathfinder experiment control system

    NASA Astrophysics Data System (ADS)

    Muders, D.; Hafok, H.; Wyrowski, F.; Polehampton, E.; Belloche, A.; König, C.; Schaaf, R.; Schuller, F.; Hatchell, J.; van der Tak, F.

    2006-08-01

    APECS is the distributed control system of the new Atacama Pathfinder EXperiment (APEX) telescope located on the Llano de Chajnantor at an altitude of 5107 m in the Atacama desert in northern Chile. APECS is based on Atacama Large Millimeter Array (ALMA) software and employs a modern, object-oriented design using the Common Object Request Broker Architecture (CORBA) as the middleware. New generic device interfaces simplify adding instruments to the control system. The Python based observer command scripting language allows using many existing software libraries and facilitates creating more complex observing modes. A new self-descriptive raw data format (Multi-Beam FITS or MBFITS) has been defined to store the multi-beam, multi-frequency data. APECS provides an online pipeline for initial calibration, observer feedback and a quick-look display. APECS is being used for regular science observations in local and remote mode since August 2005.

  10. Experiment-Based Teaching in Advanced Control Engineering

    ERIC Educational Resources Information Center

    Precup, R.-E.; Preitl, S.; Radac, M.-B.; Petriu, E. M.; Dragos, C.-A.; Tar, J. K.

    2011-01-01

    This paper discusses an experiment-based approach to teaching an advanced control engineering syllabus involving controlled plant analysis and modeling, control structures and algorithms, real-time laboratory experiments, and their assessment. These experiments are structured around the representative case of the longitudinal slip control of an…

  11. ROS Regulation During Abiotic Stress Responses in Crop Plants.

    PubMed

    You, Jun; Chan, Zhulong

    2015-01-01

    Abiotic stresses such as drought, cold, salt and heat cause reduction of plant growth and loss of crop yield worldwide. Reactive oxygen species (ROS) including hydrogen peroxide (H2O2), superoxide anions (O2 (•-)), hydroxyl radical (OH•) and singlet oxygen ((1)O2) are by-products of physiological metabolisms, and are precisely controlled by enzymatic and non-enzymatic antioxidant defense systems. ROS are significantly accumulated under abiotic stress conditions, which cause oxidative damage and eventually resulting in cell death. Recently, ROS have been also recognized as key players in the complex signaling network of plants stress responses. The involvement of ROS in signal transduction implies that there must be coordinated function of regulation networks to maintain ROS at non-toxic levels in a delicate balancing act between ROS production, involving ROS generating enzymes and the unavoidable production of ROS during basic cellular metabolism, and ROS-scavenging pathways. Increasing evidence showed that ROS play crucial roles in abiotic stress responses of crop plants for the activation of stress-response and defense pathways. More importantly, manipulating ROS levels provides an opportunity to enhance stress tolerances of crop plants under a variety of unfavorable environmental conditions. This review presents an overview of current knowledge about homeostasis regulation of ROS in crop plants. In particular, we summarize the essential proteins that are involved in abiotic stress tolerance of crop plants through ROS regulation. Finally, the challenges toward the improvement of abiotic stress tolerance through ROS regulation in crops are discussed.

  12. ROS Regulation During Abiotic Stress Responses in Crop Plants

    PubMed Central

    You, Jun; Chan, Zhulong

    2015-01-01

    Abiotic stresses such as drought, cold, salt and heat cause reduction of plant growth and loss of crop yield worldwide. Reactive oxygen species (ROS) including hydrogen peroxide (H2O2), superoxide anions (O2•-), hydroxyl radical (OH•) and singlet oxygen (1O2) are by-products of physiological metabolisms, and are precisely controlled by enzymatic and non-enzymatic antioxidant defense systems. ROS are significantly accumulated under abiotic stress conditions, which cause oxidative damage and eventually resulting in cell death. Recently, ROS have been also recognized as key players in the complex signaling network of plants stress responses. The involvement of ROS in signal transduction implies that there must be coordinated function of regulation networks to maintain ROS at non-toxic levels in a delicate balancing act between ROS production, involving ROS generating enzymes and the unavoidable production of ROS during basic cellular metabolism, and ROS-scavenging pathways. Increasing evidence showed that ROS play crucial roles in abiotic stress responses of crop plants for the activation of stress-response and defense pathways. More importantly, manipulating ROS levels provides an opportunity to enhance stress tolerances of crop plants under a variety of unfavorable environmental conditions. This review presents an overview of current knowledge about homeostasis regulation of ROS in crop plants. In particular, we summarize the essential proteins that are involved in abiotic stress tolerance of crop plants through ROS regulation. Finally, the challenges toward the improvement of abiotic stress tolerance through ROS regulation in crops are discussed. PMID:26697045

  13. Abiotic Methane Synthesis: Caveats and New Results

    NASA Astrophysics Data System (ADS)

    Zou, R.; Sharma, A.

    2005-12-01

    The role of mineral interaction with geochemical fluids under hydrothermal conditions has invoked models of geochemical synthesis of organic molecules at deep crustal conditions. Since Thomas Gold's (1992) hypothesis of the possibility of an abiotic organic synthesis, there have been several reports of hydrocarbon formation under high pressure and temperature conditions. Several previous experimental studies have recognized that small amounts of methane (and other light HC compounds) can be synthesized via catalysis by transition metals: Fe, Ni (Horita and Berndt, 1999 Science) and Cr (Foustavous and Seyfried, 2004 Science). In light of these pioneering experiments, an investigation of the feasibility of abiotic methane synthesis at higher pressure conditions in deep geological setting and the possible role of catalysis warrants a closer look. We conducted three sets of experiments in hydrothermal diamond anvil cell using FeO nanopowder, CaCO 3 and water at 300° - 600° C and 0.5 - 5 GPa : (a) with stainless steel gasket, (b) gold-lined gasket, and (c) gold-lined gasket with added Fe and Ni nanopowder. The reactions were monitored in-situ using micro-Raman spectroscopy with 532nm and 632nm lasers. The solids phases were characterized in-situ using synchrotron X-ray diffraction at CHESS-Cornell and quenched products with an electron microprobe. Interestingly, a variable amount of hydrocarbon was observed only in runs with stainless steel gasket and with Fe, Ni nanoparticles. Experiments with gold-lined reactors did not show any hydrocarbon formation. Added high resolution microscopy of the products and their textural relationship within the diamond cell with Raman spectroscopy data show that the hydrocarbon (methane and other light fractions) synthesis is a direct result of transition metal catalysis, rather than wustite - calcium carbonate reaction as recently reported by Scott et al (2004, PNAS). The author will further present new results highlighting abiotic

  14. Enhanced Attitude Control Experiment for SSTI Lewis Spacecraft

    NASA Technical Reports Server (NTRS)

    Maghami, Peoman G.

    1997-01-01

    The enhanced attitude control system experiment is a technology demonstration experiment on the NASA's small spacecraft technology initiative program's Lewis spacecraft to evaluate advanced attitude control strategies. The purpose of the enhanced attitude control system experiment is to evaluate the feasibility of designing and implementing robust multi-input/multi-output attitude control strategies for enhanced pointing performance of spacecraft to improve the quality of the measurements of the science instruments. Different control design strategies based on modern and robust control theories are being considered for the enhanced attitude control system experiment. This paper describes the experiment as well as the design and synthesis of a mixed H(sub 2)/H(sub infinity) controller for attitude control. The control synthesis uses a nonlinear programming technique to tune the controller parameters and impose robustness and performance constraints. Simulations are carried out to demonstrate the feasibility of the proposed attitude control design strategy. Introduction

  15. Controlled Space Physics Experiments using Laboratory Magnetospheres

    NASA Astrophysics Data System (ADS)

    Mauel, M. E.; Kesner, J.; Garnier, D.

    2013-12-01

    Modern society's reliance on space-based platforms for a variety of economic and geopolitical purposes makes understanding the physics of the magnetosphere and "space weather'' one of the most important applications of plasma science. During the past decade, results from the CTX and LDX laboratory magnetospheres and from the RT-1 device at University of Tokyo, we have developed techniques to explore space physics using controlled experiments in laboratory magnetospheres. This presentation briefly reviews observations from the laboratory magnetospheres at Columbia University and MIT, including adiabatic drift-resonant transport, low-frequency MHD turbulence, and the formation of high-beta plasmas with profiles similar to Earth's inner magnetosphere. First principle validation of ``whole plasma'' space weather models have been completed in relevant magnetic geometry, including the spectrum and dynamics of turbulence successfully modeled with nonlinear bounce-averaged gyrokinetic simulations. Plans to explore Alfvénic dynamics and whistler wave trapping are discussed through the achievement of higher-density plasmas using radio-frequency heating. Photographs of the laboratory magnetospheres located at MIT (top) and Columbia University (bottom).

  16. Controlled Space Physics Experiments using Laboratory Magnetospheres

    NASA Astrophysics Data System (ADS)

    Mauel, Michael; Davis, M.; Garnier, D.; Roberts, T. M.; Worstell, M.; Kesner, J.

    2013-10-01

    Modern society's reliance on space-based platforms for a variety of economic and geopolitical purposes makes understanding the physics of the magnetosphere and ``space weather'' one of the most important applications of plasma science. During the past decade, results from the CTX and LDX laboratory magnetospheres and from the RT-1 device at University of Tokyo, we have developed techniques to explore space physics using controlled experiments in laboratory magnetospheres. This presentation briefly reviews observations from the laboratory magnetospheres at Columbia University and MIT, including adiabatic drift-resonant transport, low-frequency MHD turbulence, and the formation of high-beta plasmas with profiles similar to Earth's inner magnetosphere. First principle validation of ``whole plasma'' space weather models have been completed in relevant magnetic geometry, including the spectrum and dynamics of turbulence successfully modeled with nonlinear bounce-averaged gyrokinetic simulations. Plans to explore Alfvénic dynamics and whistler wave trapping are discussed through the achievement of higher-density plasmas using radio-frequency heating. Supported by the NSF-DOE Partnership in Plasma Science.

  17. An Experiment in Linguistic Synthesis with a Fuzzy Logic Controller

    ERIC Educational Resources Information Center

    Mamdani, E. H.; Assilian, S.

    1975-01-01

    This paper describes an experiment on the "linguistic" synthesis of a controller for a model industrial plant (a steam engine). Fuzzy logic is used to convert heuristic control rules stated by a human operator into an automatic control strategy. (Author)

  18. Physics Experiments with Nintendo Wii Controllers

    ERIC Educational Resources Information Center

    Wheeler, Martyn D.

    2011-01-01

    This article provides a detailed description of the use of Nintendo Wii game controllers in physics demonstrations. The main features of the controller relevant to physics are outlined and the procedure for communicating with a PC is described. A piece of software written by the author is applied to gathering data from a controller suspended from…

  19. Abiotic Organic Chemistry in Hydrothermal Systems.

    NASA Astrophysics Data System (ADS)

    Simoneit, B. R.; Rushdi, A. I.

    2004-12-01

    Abiotic organic chemistry in hydrothermal systems is of interest to biologists, geochemists and oceanographers. This chemistry consists of thermal alteration of organic matter and minor prebiotic synthesis of organic compounds. Thermal alteration has been extensively documented to yield petroleum and heavy bitumen products from contemporary organic detritus. Carbon dioxide, carbon monoxide, ammonia and sulfur species have been used as precursors in prebiotic synthesis experiments to organic compounds. These inorganic species are common components of hot spring gases and marine hydrothermal systems. It is of interest to further test their reactivities in reductive aqueous thermolysis. We have synthesized organic compounds (lipids) in aqueous solutions of oxalic acid, and with carbon disulfide or ammonium bicarbonate at temperatures from 175-400° C. The synthetic lipids from oxalic acid solutions consisted of n-alkanols, n-alkanoic acids, n-alkyl formates, n-alkanones, n-alkenes and n-alkanes, typically to C30 with no carbon number preferences. The products from CS2 in acidic aqueous solutions yielded cyclic thioalkanes, alkyl polysulfides, and thioesters with other numerous minor compounds. The synthesis products from oxalic acid and ammonium bicarbonate solutions were homologous series of n-alkyl amides, n-alkyl amines, n-alkanes and n-alkanoic acids, also to C30 with no carbon number predominance. Condensation (dehydration) reactions also occur under elevated temperatures in aqueous medium as tested by model reactions to form amide, ester and nitrile bonds. It is concluded that the abiotic formation of aliphatic lipids, condensation products (amides, esters, nitriles, and CS2 derivatives (alkyl polysulfides, cyclic polysulfides) is possible under hydrothermal conditions and warrants further studies.

  20. Abiotic stresses affect Trichoderma harzianum T39-induced resistance to downy mildew in grapevine.

    PubMed

    Roatti, Benedetta; Perazzolli, Michele; Gessler, Cesare; Pertot, Ilaria

    2013-12-01

    Enhancement of plant defense through the application of resistance inducers seems a promising alternative to chemical fungicides for controlling crop diseases but the efficacy can be affected by abiotic factors in the field. Plants respond to abiotic stresses with hormonal signals that may interfere with the mechanisms of induced systemic resistance (ISR) to pathogens. In this study, we exposed grapevines to heat, drought, or both to investigate the effects of abiotic stresses on grapevine resistance induced by Trichoderma harzianum T39 (T39) to downy mildew. Whereas the efficacy of T39-induced resistance was not affected by exposure to heat or drought, it was significantly reduced by combined abiotic stresses. Decrease of leaf water potential and upregulation of heat-stress markers confirmed that plants reacted to abiotic stresses. Basal expression of defense-related genes and their upregulation during T39-induced resistance were attenuated by abiotic stresses, in agreement with the reduced efficacy of T39. The evidence reported here suggests that exposure of crops to abiotic stress should be carefully considered to optimize the use of resistance inducers, especially in view of future global climate changes. Expression analysis of ISR marker genes could be helpful to identify when plants are responding to abiotic stresses, in order to optimize treatments with resistance inducers in field.

  1. Biotic and Abiotic Factors Controlling Respiration Rates of Above- and Belowground Woody Debris of Fagus crenata and Quercus crispula in Japan.

    PubMed

    Jomura, Mayuko; Akashi, Yuhei; Itoh, Hiromu; Yuki, Risa; Sakai, Yoshimi; Maruyama, Yutaka

    2015-01-01

    As a large, long-term pool and source of carbon and nutrients, woody litter is an important component of forest ecosystems. The objective of this study was to estimate the effect of the factors that regulate the rate of decomposition of coarse and fine woody debris (CFWD) of dominant tree species in a cool-temperate forest in Japan. Respiration rates of dead stems, branches, and coarse and fine roots of Fagus crenata and Quercus crispula felled 4 years prior obtained in situ ranged from 20.9 to 500.1 mg CO2 [kg dry wood](-1) h(-1) in a one-time measurement in summer. Respiration rate had a significant negative relationship with diameter; in particular, that of a sample of Q. crispula with a diameter of >15 cm and substantial heartwood was low. It also had a significant positive relationship with moisture content. The explanatory variables diameter, [N], wood density, and moisture content were interrelated. The most parsimonious path model showed 14 significant correlations among 8 factors and respiration. Diameter and [C] had large negative direct effects on CFWD respiration rate, and moisture content and species had medium positive direct effects. [N] and temperature did not have direct or indirect effects, and position and wood density had indirect effects. The model revealed some interrelationships between controlling factors. We discussed the influence of the direct effects of explanatory variables and the influence especially of species and position. We speculate that the small R2 value of the most parsimonious model was probably due to the omission of microbial biomass and activity. These direct and indirect effects and interrelationships between explanatory variables could be used to develop a process-based CFWD decomposition model.

  2. Biotic and Abiotic Factors Controlling Respiration Rates of Above- and Belowground Woody Debris of Fagus crenata and Quercus crispula in Japan

    PubMed Central

    Jomura, Mayuko; Akashi, Yuhei; Itoh, Hiromu; Yuki, Risa; Sakai, Yoshimi; Maruyama, Yutaka

    2015-01-01

    As a large, long-term pool and source of carbon and nutrients, woody litter is an important component of forest ecosystems. The objective of this study was to estimate the effect of the factors that regulate the rate of decomposition of coarse and fine woody debris (CFWD) of dominant tree species in a cool-temperate forest in Japan. Respiration rates of dead stems, branches, and coarse and fine roots of Fagus crenata and Quercus crispula felled 4 years prior obtained in situ ranged from 20.9 to 500.1 mg CO2 [kg dry wood]–1 h–1 in a one-time measurement in summer. Respiration rate had a significant negative relationship with diameter; in particular, that of a sample of Q. crispula with a diameter of >15 cm and substantial heartwood was low. It also had a significant positive relationship with moisture content. The explanatory variables diameter, [N], wood density, and moisture content were interrelated. The most parsimonious path model showed 14 significant correlations among 8 factors and respiration. Diameter and [C] had large negative direct effects on CFWD respiration rate, and moisture content and species had medium positive direct effects. [N] and temperature did not have direct or indirect effects, and position and wood density had indirect effects. The model revealed some interrelationships between controlling factors. We discussed the influence of the direct effects of explanatory variables and the influence especially of species and position. We speculate that the small R2 value of the most parsimonious model was probably due to the omission of microbial biomass and activity. These direct and indirect effects and interrelationships between explanatory variables could be used to develop a process-based CFWD decomposition model. PMID:26658727

  3. Flexible manipulator control experiments and analysis

    NASA Technical Reports Server (NTRS)

    Yurkovich, S.; Ozguner, U.; Tzes, A.; Kotnik, P. T.

    1987-01-01

    Modeling and control design for flexible manipulators, both from an experimental and analytical viewpoint, are described. From the application perspective, an ongoing effort within the laboratory environment at the Ohio State University, where experimentation on a single link flexible arm is underway is described. Several unique features of this study are described here. First, the manipulator arm is slewed by a direct drive dc motor and has a rigid counterbalance appendage. Current experimentation is from two viewpoints: (1) rigid body slewing and vibration control via actuation with the hub motor, and (2) vibration suppression through the use of structure-mounted proof-mass actuation at the tip. Such an application to manipulator control is of interest particularly in design of space-based telerobotic control systems, but has received little attention to date. From an analytical viewpoint, parameter estimation techniques within the closed-loop for self-tuning adaptive control approaches are discussed. Also introduced is a control approach based on output feedback and frequency weighting to counteract effects of spillover in reduced-order model design. A model of the flexible manipulator based on experimental measurements is evaluated for such estimation and control approaches.

  4. Desiccation cracks in siliciclastic deposits: Microbial mat-related compared to abiotic sedimentary origin

    NASA Astrophysics Data System (ADS)

    Kovalchuk, Olga; Owttrim, George W.; Konhauser, Kurt O.; Gingras, Murray K.

    2017-01-01

    Siliciclastic sediment colonized by microbial mats yield a set of distinct sedimentary fabrics that are collectively called "mat-related structures (MRS)". In the rock record, versatile cracks are observed in biostabilized strata, but the mechanisms responsible for their formation remain debated. Microbially stabilized sediments produce desiccation cracks that serve as modern analogs for fossil microbial cracks. However, since both microbial mat shrinkage and clay shrinkage may contribute to the formation of these desiccation cracks, it is difficult to isolate the influence of the microbial mat on the resulting crack formation, distribution and morphology. To address this issue, we conducted a series of desiccation experiments that determine differences between microbially influenced desiccation cracks (i.e. biotic) and those formed in identical, but sterilized (i.e. abiotic) siliciclastic sediment. Three sediment mixtures were used: (1) very fine-sized sand, (2) mixed (ungraded) silt/clay, and (3) normally graded silt/clay. In all of the experiments, the water-rich microbial mat contracted substantially while drying, producing isolated pockets of shallow, but wide cracks, the distribution of which was controlled by heterogeneities in the mat structure and thickness variations of the mat. In the clay-poor substratum, the microbial mat was the only crack-forming mechanism, while in the clay-rich substrata (experiments 2 and 3) desiccation cracks were more strongly influenced by clay shrinkage. The abiotic clay-rich sediment produced a polygonal network of deep cracks intersecting at 90-120o junctions. In the biotic clay-rich experiments, the microbial mat modified these desiccation features by withstanding crack propagation or by producing curled-up crack polygon margins. Even though a microbial mat shrinks substantially with desiccation, its cohesive nature and heterogeneous distribution prevents the formation of a regular crack network, but its shallow penetration

  5. Power and control choice in aquatic experiments with solvents.

    PubMed

    Green, John W

    2014-04-01

    Aquatic toxicology experiments to determine the effects of chemicals sometimes require the use of a carrier solvent. Such experiments typically include both a negative (water) control group and a solvent control group. False positive rates and power to detect treatment effects in such experiments are compared for six possible strategies for deciding the appropriate control or controls for comparison. The main purpose of the present study is to determine the best use of the two controls in statistical analysis. A secondary purpose is to determine purely on statistical grounds whether both controls are actually needed. The evidence supports using either the solvent control only in all cases or a sequential strategy of combining the water and solvent controls unless the two controls are found to be statistically significantly different, in which case only the solvent control should be used. These results extend, and in some ways contradict, a recently published simulation study.

  6. Millikan's oil-drop experiment as a remotely controlled laboratory

    NASA Astrophysics Data System (ADS)

    Eckert, Bodo; Gröber, Sebastian; Vetter, Martin; Jodl, Hans-Jörg

    2012-09-01

    The Millikan oil-drop experiment, to determine the elementary electrical charge e and the quantization of charge Q = n · e, is an essential experiment in physics teaching but it is hardly performed in class for several reasons. Therefore, we offer this experiment as a remotely controlled laboratory (RCL). We describe the interactivity of the experiment and the quality of measurements. The added value to offer the Millikan experiment as an RCL is pointed out.

  7. Skylab experiment performance evaluation manual. Appendix R: Experiment T020 foot controlled maneuvering unit (MSFC)

    NASA Technical Reports Server (NTRS)

    Tonetti, B. B.

    1972-01-01

    A series of analyses for experiment T020, foot controlled maneuvering unit (MSFC), to be used for evaluating the performance of the Skylab corollary experiments under preflight, inflight and post-flight conditions is reported. Experiment contingency plan procedure and malfunction analyses are presented in order to assist in making the experiment operationally successful.

  8. Description of the Spacecraft Control Laboratory Experiment (SCOLE) facility

    NASA Technical Reports Server (NTRS)

    Williams, Jeffrey P.; Rallo, Rosemary A.

    1987-01-01

    A laboratory facility for the study of control laws for large flexible spacecraft is described. The facility fulfills the requirements of the Spacecraft Control Laboratory Experiment (SCOLE) design challenge for laboratory experiments, which will allow slew maneuvers and pointing operations. The structural apparatus is described in detail sufficient for modelling purposes. The sensor and actuator types and characteristics are described so that identification and control algorithms may be designed. The control implementation computer and real-time subroutines are also described.

  9. Description of the Spacecraft Control Laboratory Experiment (SCOLE) facility

    NASA Technical Reports Server (NTRS)

    Williams, Jeffrey P.; Rallo, Rosemary A.

    1987-01-01

    A laboratory facility for the study of control laws for large flexible spacecraft is described. The facility fulfills the requirements of the Spacecraft Control Laboratory Experiment (SCOLE) design challenge for a laboratory experiment, which will allow slew maneuvers and pointing operations. The structural apparatus is described in detail sufficient for modelling purposes. The sensor and actuator types and characteristics are described so that identification and control algorithms may be designed. The control implementation computer and real-time subroutines are also described.

  10. Evaluating reaction pathways of hydrothermal abiotic organic synthesis at elevated temperatures and pressures using carbon isotopes

    NASA Astrophysics Data System (ADS)

    Fu, Qi; Socki, Richard A.; Niles, Paul B.

    2015-04-01

    Experiments were performed to better understand the role of environmental factors on reaction pathways and corresponding carbon isotope fractionations during abiotic hydrothermal synthesis of organic compounds using piston cylinder apparatus at 750 °C and 5.5 kbars. Chemical compositions of experimental products and corresponding carbon isotopic values were obtained by a Pyrolysis-GC-MS-IRMS system. Alkanes (methane and ethane), straight-chain saturated alcohols (ethanol and n-butanol) and monocarboxylic acids (formic and acetic acids) were generated with ethanol being the only organic compound with higher δ13C than CO2. CO was not detected in experimental products owing to the favorable water-gas shift reaction under high water pressure conditions. The pattern of δ13C values of CO2, carboxylic acids and alkanes are consistent with their equilibrium isotope relationships: CO2 > carboxylic acids > alkanes, but the magnitude of the fractionation among them is higher than predicted isotope equilibrium values. In particular, the isotopic fractionation between CO2 and CH4 remained constant at ∼31‰, indicating a kinetic effect during CO2 reduction processes. No "isotope reversal" of δ13C values for alkanes or carboxylic acids was observed, which indicates a different reaction pathway than what is typically observed during Fischer-Tropsch synthesis under gas phase conditions. Under constraints imposed in experiments, the anomalous 13C isotope enrichment in ethanol suggests that hydroxymethylene is the organic intermediate, and that the generation of other organic compounds enriched in 12C were facilitated by subsequent Rayleigh fractionation of hydroxymethylene reacting with H2 and/or H2O. Carbon isotope fractionation data obtained in this study are instrumental in assessing the controlling factors on abiotic formation of organic compounds in hydrothermal systems. Knowledge on how environmental conditions affect reaction pathways of abiotic synthesis of organic

  11. Dawn Spacecraft Reaction Control System Flight Experience

    NASA Technical Reports Server (NTRS)

    Mizukami, Masashi; Nakazono, Barry

    2014-01-01

    The NASA Dawn spacecraft mission is studying conditions and processes of the solar system's earliest epoch by investigating two protoplanets remaining intact since their formations, Ceres and Vesta. Launch was in 2007. Ion propulsion is used to fly to and enter orbit around Vesta, depart Vesta and fly to Ceres, and enter orbit around Ceres. A conventional blowdown hydrazine reaction control system (RCS) is used to provide external torques for attitude control. Reaction wheel assemblies were intended to provide attitude control in most cases. However, the spacecraft experienced one, then two apparent failures of reaction wheels. Also, similar thrusters experienced degradation in a long life application on another spacecraft. Those factors led to RCS being operated in ways completely different than anticipated prior to launch. Numerous mitigations and developments needed to be implemented. The Vesta mission was fully successful. Even with the compromises necessary due to those anomalies, the Ceres mission is also projected to be feasible.

  12. Circulation control lift generation experiment: Hardware development

    NASA Technical Reports Server (NTRS)

    Panontin, T. L.

    1985-01-01

    A circulation control airfoil and its accompanying hardware were developed to allow the investigation of lift generation that is independent of airfoil angle of attack and relative flow velocity. The test equipment, designed for use in a water tunnel, includes the blown airfoil, the support systems for both flow visualization and airfoil load measurement, and the fluid control system, which utilizes hydraulic technology. The primary design tasks, the selected solutions, and the unforseen problems involved in the development of these individual components of hardware are described.

  13. Experiments with a Magnetically Controlled Pendulum

    ERIC Educational Resources Information Center

    Kraftmakher, Yaakov

    2007-01-01

    A magnetically controlled pendulum is used for observing free and forced oscillations, including nonlinear oscillations and chaotic motion. A data-acquisition system stores the data and displays time series of the oscillations and related phase plane plots, Poincare maps, Fourier spectra and histograms. The decay constant of the pendulum can be…

  14. Aeroassist flight experiment guidance, navigation and control

    NASA Technical Reports Server (NTRS)

    Brand, Timothy J.; Engel, Albert G.

    1986-01-01

    The Aeroassist Flight Experiment scheduled for the early 1990's will demonstrate the use of a low L/D lifting brake using aerodynamic drag to return a spacecraft from a high energy to a low earth orbit. The experimental vehicle will be deployed and retrieved by the Shuttle Orbiter. This paper reviews some of the challenges, problems, and solutions encountered to date during guidance system development, with emphasis on technology advances which will benefit an operational Orbit Transfer Vehicle (OTV). Key factors to be discussed include guidance alternatives, aerodynamic modeling, navigation requirements, the impact of atmospheric uncertainties, and flight profile alternatives considered during initial planning.

  15. Abiotic degradation of plastic films

    NASA Astrophysics Data System (ADS)

    Ángeles-López, Y. G.; Gutiérrez-Mayen, A. M.; Velasco-Pérez, M.; Beltrán-Villavicencio, M.; Vázquez-Morillas, A.; Cano-Blanco, M.

    2017-01-01

    Degradable plastics have been promoted as an option to mitigate the environmental impacts of plastic waste. However, there is no certainty about its degradability under different environmental conditions. The effect of accelerated weathering (AW), natural weathering (NW) and thermal oxidation (TO) on different plastics (high density polyethylene, HDPE; oxodegradable high density polyethylene, HDPE-oxo; compostable plastic, Ecovio ® metalized polypropylene, PP; and oxodegradable metalized polypropylene, PP-oxo) was studied. Plastics films were exposed to AW per 110 hours; to NW per 90 days; and to TO per 30 days. Plastic films exposed to AW and NW showed a general loss on mechanical properties. The highest reduction in elongation at break on AW occurred to HDPE-oxo (from 400.4% to 20.9%) and was higher than 90% for HDPE, HDPE-oxo, Ecovio ® and PP-oxo in NW. No substantial evidence of degradation was found on plastics exposed to TO. Oxo-plastics showed higher degradation rates than their conventional counterparts, and the compostable plastic was resistant to degradation in the studied abiotic conditions. This study shows that degradation of plastics in real life conditions will vary depending in both, their composition and the environment.

  16. 2. VIEW OF THE EXPERIMENT CONTROL PANEL IN 1970. THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW OF THE EXPERIMENT CONTROL PANEL IN 1970. THE NUCLEAR SAFETY GROUP CONDUCTED ABOUT 1,700 CRITICAL MASS EXPERIMENTS USING URANIUM AND PLUTONIUM IN SOLUTIONS (900 TESTS), COMPACTED POWDER (300), AND METALLIC FORMS (500). ALL 1,700 CRITICALITY ASSEMBLIES WERE CONTROLLED FROM THIS PANEL. - Rocky Flats Plant, Critical Mass Laboratory, Intersection of Central Avenue & 86 Drive, Golden, Jefferson County, CO

  17. Millikan's Oil-Drop Experiment as a Remotely Controlled Laboratory

    ERIC Educational Resources Information Center

    Eckert, Bodo; Grober, Sebastian; Vetter, Martin; Jodl, Hans-Jorg

    2012-01-01

    The Millikan oil-drop experiment, to determine the elementary electrical charge e and the quantization of charge Q = n [middle dot] e, is an essential experiment in physics teaching but it is hardly performed in class for several reasons. Therefore, we offer this experiment as a remotely controlled laboratory (RCL). We describe the interactivity…

  18. The run control and monitoring system of the CMS experiment

    SciTech Connect

    Bauer, Gerry; Boyer, Vincent; Branson, James; Brett, Angela; Cano, Eric; Carboni, Andrea; Ciganek, Marek; Cittolin, Sergio; O'Dell, Vivian; Erhan, Samim; Gigi, Dominique; /CERN /Kyungpook Natl. U. /MIT /UCLA /CERN /INFN, Legnaro

    2007-10-01

    The CMS experiment at the LHC at CERN will start taking data in 2008. To configure, control and monitor the experiment during data-taking the Run Control and Monitoring System (RCMS) was developed. This paper describes the architecture and the technology used to implement the RCMS, as well as the deployment and commissioning strategy of this important component of the online software for the CMS experiment.

  19. Experience with subsea well control systems

    SciTech Connect

    Coltharp, E.D.; Coffelt, D.E.

    1981-01-01

    Since 1969, Conoco Inc. has installed ten offshore wells and one land test of subsea completion systems. These wells consist of four single zone oil wells plus one water injection well with Thru Flowline (TFL) pumpdown capability and three single zone gas wells plus three dual zone gas wells utilizing the ''Plain Jane'' wellheads without TFL capability. The control systems for these wells have varied from an electro-hydraulic sequential system to a straight discrete hydraulic system. This paper deals with the design, installation, and operational problems encountered and the remedial procedures taken to solve the problems to date.

  20. Mismatch in microbial food webs: predators but not prey perform better in their local biotic and abiotic conditions.

    PubMed

    Parain, Elodie C; Gravel, Dominique; Rohr, Rudolf P; Bersier, Louis-Félix; Gray, Sarah M

    2016-07-01

    Understanding how trophic levels respond to changes in abiotic and biotic conditions is key for predicting how food webs will react to environmental perturbations. Different trophic levels may respond disproportionately to change, with lower levels more likely to react faster, as they typically consist of smaller-bodied species with higher reproductive rates. This response could cause a mismatch between trophic levels, in which predators and prey will respond differently to changing abiotic or biotic conditions. This mismatch between trophic levels could result in altered top-down and bottom-up control and changes in interaction strength. To determine the possibility of a mismatch, we conducted a reciprocal-transplant experiment involving Sarracenia purpurea food webs consisting of bacterial communities as prey and a subset of six morphologically similar protozoans as predators. We used a factorial design with four temperatures, four bacteria and protozoan biogeographic origins, replicated four times. This design allowed us to determine how predator and prey dynamics were altered by abiotic (temperature) conditions and biotic (predators paired with prey from either their local or non-local biogeographic origin) conditions. We found that prey reached higher densities in warmer temperature regardless of their temperature of origin. Conversely, predators achieved higher densities in the temperature condition and with the prey from their origin. These results confirm that predators perform better in abiotic and biotic conditions of their origin while their prey do not. This mismatch between trophic levels may be especially significant under climate change, potentially disrupting ecosystem functioning by disproportionately affecting top-down and bottom-up control.

  1. Computerized monitoring and control of experiments in space

    NASA Technical Reports Server (NTRS)

    Janisch, Thomas V.

    1988-01-01

    The computer subsystem of the Villanova University GAS (Get Away Special) experiment apparatus is discussed. The function of the computer subsystem is to provide data acquisition and control system support to the experiments. The computer subsystem will provide high availability, low power consumption and highly reliable data retention. The general layout of the subsystem provides for redundant processing units, control modules, and multiple data acquisition modules. Each of the two redundant processing units will be composed of a microprocessor, control logic, PROM, RAM, non-volitile memory, timers, self-check logic and data ports to the data acquisition and control modules. One unit will control the experiment while the other shadows the primary unit operation. The data acquisition module gathers data from the experiment. The data is transfered to the processing unit in digital form. The control module validates the data, decodes it and executes the command.

  2. Abiotic stress and the plant circadian clock

    PubMed Central

    Sanchez, Alfredo; Shin, Jieun

    2011-01-01

    In this review, we focus on the interaction between the circadian clock of higher plants to that of metabolic and physiological processes that coordinate growth and performance under a predictable, albeit changing environment. In this, the phytochrome and cryptochrome photoreceptors have shown to be important, but not essential for oscillator control under diurnal cycles of light and dark. From this foundation, we will examine how emerging findings have firmly linked the circadian clock, as a central mediator in the coordination of metabolism, to maintain homeostasis. This occurs by oscillator synchronization of global transcription, which leads to a dynamic control of a host of physiological processes. These include the determination of the levels of primary and secondary metabolites, and the anticipation of future environmental stresses, such as mid-day drought and midnight coldness. Interestingly, metabolic and stress cues themselves appear to feedback on oscillator function. In such a way, the circadian clock of plants and abiotic-stress tolerance appear to be firmly interconnected processes. PMID:21325898

  3. Microbial Hub Taxa Link Host and Abiotic Factors to Plant Microbiome Variation

    PubMed Central

    Agler, Matthew T.; Ruhe, Jonas; Kroll, Samuel; Morhenn, Constanze; Kim, Sang-Tae; Weigel, Detlef; Kemen, Eric M.

    2016-01-01

    Plant-associated microorganisms have been shown to critically affect host physiology and performance, suggesting that evolution and ecology of plants and animals can only be understood in a holobiont (host and its associated organisms) context. Host-associated microbial community structures are affected by abiotic and host factors, and increased attention is given to the role of the microbiome in interactions such as pathogen inhibition. However, little is known about how these factors act on the microbial community, and especially what role microbe–microbe interaction dynamics play. We have begun to address this knowledge gap for phyllosphere microbiomes of plants by simultaneously studying three major groups of Arabidopsis thaliana symbionts (bacteria, fungi and oomycetes) using a systems biology approach. We evaluated multiple potential factors of microbial community control: we sampled various wild A. thaliana populations at different times, performed field plantings with different host genotypes, and implemented successive host colonization experiments under lab conditions where abiotic factors, host genotype, and pathogen colonization was manipulated. Our results indicate that both abiotic factors and host genotype interact to affect plant colonization by all three groups of microbes. Considering microbe–microbe interactions, however, uncovered a network of interkingdom interactions with significant contributions to community structure. As in other scale-free networks, a small number of taxa, which we call microbial “hubs,” are strongly interconnected and have a severe effect on communities. By documenting these microbe–microbe interactions, we uncover an important mechanism explaining how abiotic factors and host genotypic signatures control microbial communities. In short, they act directly on “hub” microbes, which, via microbe–microbe interactions, transmit the effects to the microbial community. We analyzed two “hub” microbes (the

  4. An abiotic analogue of the nuclear pore complex hydrogel.

    PubMed

    Bird, Sean P; Baker, Lane A

    2011-09-12

    We describe an abiotic hydrogel that mimics selectivity of the nuclear pore complex. Copolymerization of peptide tetramers (phenylalanine-serine-phenylalanine-glycine, FSFG) with acrylamide results in hydrophobic interactions significant enough to allow the formation of freestanding hydrogel structures. Incorporation of FSFG motifs also renders the hydrogels selective. Selective binding of importins and nuclear transport receptor-cargo complexes is qualitatively demonstrated and compared with polyacrylamide, hydrogels prepared from a control peptide, and hydrogels prepared from the nuclear pore complex protein Nsp1. These abiotic hydrogels will enable further studies of the unique transport mechanisms of the nuclear pore complex and provide an interesting paradigm for the future development of synthetic platforms for separations and selective interfaces.

  5. SUMO, a heavyweight player in plant abiotic stress responses.

    PubMed

    Castro, Pedro Humberto; Tavares, Rui Manuel; Bejarano, Eduardo R; Azevedo, Herlânder

    2012-10-01

    Protein post-translational modifications diversify the proteome and install new regulatory levels that are crucial for the maintenance of cellular homeostasis. Over the last decade, the ubiquitin-like modifying peptide small ubiquitin-like modifier (SUMO) has been shown to regulate various nuclear processes, including transcriptional control. In plants, the sumoylation pathway has been significantly implicated in the response to environmental stimuli, including heat, cold, drought, and salt stresses, modulation of abscisic acid and other hormones, and nutrient homeostasis. This review focuses on the emerging importance of SUMO in the abiotic stress response, summarizing the molecular implications of sumoylation and emphasizing how high-throughput approaches aimed at identifying the full set of SUMO targets will greatly enhance our understanding of the SUMO-abiotic stress association.

  6. In silico pooling of ChIP-seq control experiments.

    PubMed

    Sun, Guannan; Srinivasan, Rajini; Lopez-Anido, Camila; Hung, Holly A; Svaren, John; Keleş, Sündüz

    2014-01-01

    As next generation sequencing technologies are becoming more economical, large-scale ChIP-seq studies are enabling the investigation of the roles of transcription factor binding and epigenome on phenotypic variation. Studying such variation requires individual level ChIP-seq experiments. Standard designs for ChIP-seq experiments employ a paired control per ChIP-seq sample. Genomic coverage for control experiments is often sacrificed to increase the resources for ChIP samples. However, the quality of ChIP-enriched regions identifiable from a ChIP-seq experiment depends on the quality and the coverage of the control experiments. Insufficient coverage leads to loss of power in detecting enrichment. We investigate the effect of in silico pooling of control samples within multiple biological replicates, multiple treatment conditions, and multiple cell lines and tissues across multiple datasets with varying levels of genomic coverage. Our computational studies suggest guidelines for performing in silico pooling of control experiments. Using vast amounts of ENCODE data, we show that pairwise correlations between control samples originating from multiple biological replicates, treatments, and cell lines/tissues can be grouped into two classes representing whether or not in silico pooling leads to power gain in detecting enrichment between the ChIP and the control samples. Our findings have important implications for multiplexing samples.

  7. Control of large collector arrays: The SSPS experience

    NASA Astrophysics Data System (ADS)

    Carmona, R.; Martin, J. G.

    Experience gained in the control of the distributed collector fields at the IEA Small Solar Power Systems project may be of value in the design of control systems for future large arrays. The project experience with analog and digital systems is discussed, as are details on the improvements that were made and the lessons learned. A priority item in this year's efforts on site is the evaluation of the potential for fully automatic operation, with a suitable control algorithm, of a reliable collector array. Preliminary results from dynamic models of the fields in terms of lumped and distributed parameters are given. Adaptive controls are discussed.

  8. Alkylation of Chlorobenzene. An Experiment Illustrating Kinetic versus Thermodynamic Control.

    ERIC Educational Resources Information Center

    Kolb, Kenneth; And Others

    1988-01-01

    Describes an experiment which illustrates the kinetic versus thermodynamic control of chemical reactions for organic chemistry students. Considers the laboratory procedures including the isolation of both the kinetic and thermodynamic products. (CW)

  9. Sexual Experience and Responses to a Birth Control Film.

    ERIC Educational Resources Information Center

    Herold, Edward S.; Thomas, Roger E.

    1980-01-01

    The relationship between sexual experience and contraceptive attitudinal responses to a birth control film is examined. Significant group differences were found regarding reinforcement of sexual attitudes but not for contraceptive attitudes. (JMF)

  10. A feasibility study of orbiter flight control experiments

    NASA Technical Reports Server (NTRS)

    Geissler, W. H.

    1978-01-01

    The results of a feasibility study of orbiter flight control experiments performed are summarized. Feasibility studies were performed on a group of 14 experiments selected from a candidate list of 35 submitted to the study contractor by the flight control community. Concepts and requirements were developed for the 14 selected experiments and they were ranked on a basis of technical value, feasibility, and cost. It was concluded that all the selected experiments can be considered as potential candidates for the Orbiter Experiment program, which is being formulated for the Orbiter Flight Tests and subsequent operational flights, regardless of the relative ranking established during the study. None of the selected experiments has significant safety implications and the cost of most was estimated to be less than $200K.

  11. Experience of Control and Student Satisfaction with Higher Education Services

    ERIC Educational Resources Information Center

    Lee, Jungki; Anantharaman, Sekhar

    2013-01-01

    Although the delivery of satisfactory services is an important strategic goal in many colleges, students are known to face challenges and experience a significant amount of stress during their school life. This study proposes and tests students' experience of control over their college life as a promising factor that would enhance their…

  12. Control of Physiological Experiments Using a Hybrid Computer.

    ERIC Educational Resources Information Center

    Jermann, William H.; Watkins, Melinda G.

    1980-01-01

    Described is the use of a general purpose hybrid computer in controlling and monitoring certain experiments on human subjects. In these investigations, researchers produce various sensory stimuli and measure participants' responses as voltage produced at a scalp node. The computer system discussed facilitates implementation of these experiments as…

  13. Enhanced bacoside production in shoot cultures of Bacopa monnieri under the influence of abiotic elicitors.

    PubMed

    Sharma, Munish; Ahuja, Ashok; Gupta, Rajinder; Mallubhotla, Sharada

    2015-01-01

    The effect of different abiotic elicitors [jasmonic acid, copper sulphate (CuSO4) and salicyclic acid] at varying concentrations on the stimulation of biomass and bacoside production in in vitro Bacopa monnieri shoot culture was studied. A systematic study conducted over a period of 35 days indicated that the maximum bacoside production (6.74 mg g(-1) dry weight (DW)) was obtained after a lag of 7 days and thereafter, the content decreased gradually to again increase at 28 days (5.91 mg g(-1) DW). Therefore, elicitation experiments were carried out over a period of 3, 6 and 9 days. The shoot cultures treated with 45 mg L(-1) of CuSO4 exhibited the highest bacoside content of 8.73 mg g(-1) DW (∼1.42-fold higher) than in control cultures (6.14 mg g(-1) DW). This study indicates the effectiveness of abiotic elicitation on bacoside production in in vitro shoot cultures of this medicinally important herb known for its memory-enhancing properties.

  14. Surface chemistry allows for abiotic precipitation of dolomite at low temperature

    NASA Astrophysics Data System (ADS)

    Roberts, Jennifer A.; Kenward, Paul A.; Fowle, David A.; Goldstein, Robert H.; González, Luis A.; Moore, David S.

    2013-09-01

    Although the mineral dolomite is abundant in ancient low-temperature sedimentary systems, it is scarce in modern systems below 50 °C. Chemical mechanism(s) enhancing its formation remain an enigma because abiotic dolomite has been challenging to synthesize at low temperature in laboratory settings. Microbial enhancement of dolomite precipitation at low temperature has been reported; however, it is still unclear exactly how microorganisms influence reaction kinetics. Here we document the abiotic synthesis of low-temperature dolomite in laboratory experiments and constrain possible mechanisms for dolomite formation. Ancient and modern seawater solution compositions, with identical pH and pCO2, were used to precipitate an ordered, stoichiometric dolomite phase at 30 °C in as few as 20 d. Mg-rich phases nucleate exclusively on carboxylated polystyrene spheres along with calcite, whereas aragonite forms in solution via homogeneous nucleation. We infer that Mg ions are complexed and dewatered by surface-bound carboxyl groups, thus decreasing the energy required for carbonation. These results indicate that natural surfaces, including organic matter and microbial biomass, possessing a high density of carboxyl groups may be a mechanism by which ordered dolomite nuclei form. Although environments rich in organic matter may be of interest, our data suggest that sharp biogeochemical interfaces that promote microbial death, as well as those with high salinity may, in part, control carboxyl-group density on organic carbon surfaces, consistent with origin of dolomites from microbial biofilms, as well as hypersaline and mixing zone environments.

  15. Surface chemistry allows for abiotic precipitation of dolomite at low temperature.

    PubMed

    Roberts, Jennifer A; Kenward, Paul A; Fowle, David A; Goldstein, Robert H; González, Luis A; Moore, David S

    2013-09-03

    Although the mineral dolomite is abundant in ancient low-temperature sedimentary systems, it is scarce in modern systems below 50 °C. Chemical mechanism(s) enhancing its formation remain an enigma because abiotic dolomite has been challenging to synthesize at low temperature in laboratory settings. Microbial enhancement of dolomite precipitation at low temperature has been reported; however, it is still unclear exactly how microorganisms influence reaction kinetics. Here we document the abiotic synthesis of low-temperature dolomite in laboratory experiments and constrain possible mechanisms for dolomite formation. Ancient and modern seawater solution compositions, with identical pH and pCO2, were used to precipitate an ordered, stoichiometric dolomite phase at 30 °C in as few as 20 d. Mg-rich phases nucleate exclusively on carboxylated polystyrene spheres along with calcite, whereas aragonite forms in solution via homogeneous nucleation. We infer that Mg ions are complexed and dewatered by surface-bound carboxyl groups, thus decreasing the energy required for carbonation. These results indicate that natural surfaces, including organic matter and microbial biomass, possessing a high density of carboxyl groups may be a mechanism by which ordered dolomite nuclei form. Although environments rich in organic matter may be of interest, our data suggest that sharp biogeochemical interfaces that promote microbial death, as well as those with high salinity may, in part, control carboxyl-group density on organic carbon surfaces, consistent with origin of dolomites from microbial biofilms, as well as hypersaline and mixing zone environments.

  16. Abiotic U(VI) Reduction by Sorbed Fe(II) on Natural Sediments

    SciTech Connect

    Fox, Patricia M.; Davis, James A.; Kukkadapu, Ravi K.; Singer, David M.; Bargar, John R.; Williams, Kenneth H.

    2013-09-15

    Laboratory experiments were performed as a function of aqueous Fe(II) concentration to determine the uptake and oxidation of Fe(II), and Fe(II)-mediated abiotic reduction of U(VI) by aquifer sediments from the Rifle IFRC field site in Colorado, USA. Mössbauer analysis of the sediments spiked with aqueous 57Fe(II) showed that 57Fe(II) was oxidized on the mineral surfaces to 57Fe(III) and most likely formed a nano-particulate Fe(III)-oxide or ferrihydrite-like phase. The extent of 57Fe oxidation decreased with increasing 57Fe(II) uptake, such that 100 % was oxidized at 7.3 μmol/g Fe and 52 % at 39.6 μmol/g Fe, indicating that the sediments had a finite capacity for oxidation of Fe(II). Abiotic U(VI) reduction was observed by XANES spectroscopy only when the Fe(II) uptake was greater than approximately 20 μmol/g and surface-bound Fe(II) was present. The level of U(VI) reduction increased with increasing Fe(II)- loading above this level to a maximum of 18 and 36 % U(IV) at pH 7.2 (40.7 μmol/g Fe) and 8.3 (56.1 μmol/g Fe), respectively in the presence of 400 ppm CO2. Greater U(VI) reduction was observed in CO2 free systems [up to 44 and 54 % at pH 7.2 (17.3 μmol/g Fe) and 8.3 (54.8 μmol/g Fe), respectively] compared to 400 ppm CO2 systems, presumably due to differences in aqueous U(VI) speciation. While pH affects the amount of Fe(II) uptake onto the solid phase, with greater Fe(II) uptake at higher pH, similar amounts of U(VI) reduction were observed at pH 7.2 and 8.3 for a similar Fe(II) uptake. Thus, it appears that abiotic U(VI) reduction is controlled primarily by Fe(II) concentration and aqueous U(VI) speciation. The range of Fe(II) loadings tested in this study are within the range observed in bioreduced sediments, suggesting that Fe(II)-mediated abiotic U(VI) reduction may indeed play a role in field settings.

  17. A Process Dynamics and Control Experiment for the Undergraduate Laboratory

    ERIC Educational Resources Information Center

    Spencer, Jordan L.

    2009-01-01

    This paper describes a process control experiment. The apparatus includes a three-vessel glass flow system with a variable flow configuration, means for feeding dye solution controlled by a stepper-motor driven valve, and a flow spectrophotometer. Students use impulse response data and nonlinear regression to estimate three parameters of a model…

  18. Expectations and Experience: Dissociable Bases for Cognitive Control?

    ERIC Educational Resources Information Center

    Bugg, Julie M.; Diede, Nathaniel T.; Cohen-Shikora, Emily R.; Selmeczy, Diana

    2015-01-01

    Classic theories emphasized the role of expectations in the intentional control of attention and action. However, recent theorizing has implicated experience-dependent, online adjustments as the primary basis for cognitive control--adjustments that appear to be implicit (Blais, Harris, Guerrero, & Bunge, 2012). The purpose of the current study…

  19. A deployable structure and solar array controls experiment for STEP

    NASA Technical Reports Server (NTRS)

    Nishimoto, T. S.

    1984-01-01

    A candidate configuration for a controls experiment on the Space Technology Experiments Platform (STEP) is described. The elements of the experiment are the mast, the solar array, and an articulation module between the two. The characteristic dimensions are very compatible for integration on a pallet such a STEP's proposed configuration. The controls' objective would be the measurement of orbiter interaction as well as the system identification of the appendages. The flight experiment configuration would also provide a test bed for various active vibration controls concepts. The instrumentation being considered would measure accelerations, strains, displacements, and temperatures. The deployable mast has eight elements defining a structural bay. Uniaxial measurements would be required to define loads at a cross section of the structure. Displacements due to thermal distortion of the mast and the local state of the solar concentrator may be measured by an optical ranging technique from the orbiter aft flight deck.

  20. Thermal control surfaces experiment: Initial flight data analysis

    NASA Technical Reports Server (NTRS)

    Wilkes, Donald R.; Hummer, Leigh L.

    1991-01-01

    The behavior of materials in the space environment continues to be a limiting technology for spacecraft and experiments. The thermal control surfaces experiment (TCSE) aboard the Long Duration Exposure Facility (LDEF) is the most comprehensive experiment flown to study the effects of the space environment on thermal control surfaces. Selected thermal control surfaces were exposed to the LDEF orbital environment and the effects of this exposure were measured. The TCSE combined in-space orbital measurements with pre and post-flight analyses of flight materials to determine the effects of long term space exposure. The TCSE experiment objective, method, and measurements are described along with the results of the initial materials analysis. The TCSE flight system and its excellent performance on the LDEF mission is described. A few operational anomalies were encountered and are discussed.

  1. Active aerodynamic control of wake-airfoil interaction noise - Experiment

    NASA Astrophysics Data System (ADS)

    Simonich, J. C.; Lavrich, P. L.; Sofrin, T. G.; Topol, D. A.

    A proof of concept experiment is conducted that shows the potential for active aerodynamic control of rotor wake/stator interaction noise in a simplified manner. A single airfoil model representing the stator was fitted with a moveable trailing edge flap controlled by a servo motor. The control system moves the motor driven flap in the correct angular displacement phase and rate to reduce the unsteady load on the airfoil during the wake interaction.

  2. Limits of control: the effects of uncontrollability experiences on the efficiency of attentional control.

    PubMed

    Bukowski, Marcin; Asanowicz, Dariusz; Marzecová, Anna; Lupiáñez, Juan

    2015-01-01

    Two experiments were conducted to explore the effects of experiencing uncontrollability on the efficiency of attentional control. The experience of uncontrollability was induced either by unsolvable tasks (Experiment 1) or by tasks in which non-contingent feedback was provided (Experiment 2). A version of the Attentional Network Test-Interactions with an additional measure of vigilance (ANTI-V) was used to evaluate the efficiency of the attentional networks (i.e., alerting, orienting, and executive). Results of both experiments revealed a decreased efficiency of executive attention in participants who experienced stable control deprivation but no negative effects in participants who were able to restore their sense of previously deprived control. Additionally, when participants were asked to perform unsolvable tasks and did not receive feedback (Experiment 1), detrimental effects on the orienting network and vigilance were observed. The motivational and cognitive mechanisms underlying the effects of various uncontrollability experiences on conflict resolution and attentional control are discussed.

  3. Nucleotide Selectivity in Abiotic RNA Polymerization Reactions.

    PubMed

    Coari, Kristin M; Martin, Rebecca C; Jain, Kopal; McGown, Linda B

    2017-02-03

    In order to establish an RNA world on early Earth, the nucleotides must form polymers through chemical rather than biochemical reactions. The polymerization products must be long enough to perform catalytic functions, including self-replication, and to preserve genetic information. These functions depend not only on the length of the polymers, but also on their sequences. To date, studies of abiotic RNA polymerization generally have focused on routes to polymerization of a single nucleotide and lengths of the homopolymer products. Less work has been done the selectivity of the reaction toward incorporation of some nucleotides over others in nucleotide mixtures. Such information is an essential step toward understanding the chemical evolution of RNA. To address this question, in the present work RNA polymerization reactions were performed in the presence of montmorillonite clay catalyst. The nucleotides included the monophosphates of adenosine, cytosine, guanosine, uridine and inosine. Experiments included reactions of mixtures of an imidazole-activated nucleotide (ImpX) with one or more unactivated nucleotides (XMP), of two or more ImpX, and of XMP that were activated in situ in the polymerization reaction itself. The reaction products were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify the lengths and nucleotide compositions of the polymerization products. The results show that the extent of polymerization, the degree of heteropolymerization vs. homopolymerization, and the composition of the polymeric products all vary among the different nucleotides and depend upon which nucleotides and how many different nucleotides are present in the mixture.

  4. Nucleotide Selectivity in Abiotic RNA Polymerization Reactions

    NASA Astrophysics Data System (ADS)

    Coari, Kristin M.; Martin, Rebecca C.; Jain, Kopal; McGown, Linda B.

    2017-02-01

    In order to establish an RNA world on early Earth, the nucleotides must form polymers through chemical rather than biochemical reactions. The polymerization products must be long enough to perform catalytic functions, including self-replication, and to preserve genetic information. These functions depend not only on the length of the polymers, but also on their sequences. To date, studies of abiotic RNA polymerization generally have focused on routes to polymerization of a single nucleotide and lengths of the homopolymer products. Less work has been done the selectivity of the reaction toward incorporation of some nucleotides over others in nucleotide mixtures. Such information is an essential step toward understanding the chemical evolution of RNA. To address this question, in the present work RNA polymerization reactions were performed in the presence of montmorillonite clay catalyst. The nucleotides included the monophosphates of adenosine, cytosine, guanosine, uridine and inosine. Experiments included reactions of mixtures of an imidazole-activated nucleotide (ImpX) with one or more unactivated nucleotides (XMP), of two or more ImpX, and of XMP that were activated in situ in the polymerization reaction itself. The reaction products were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify the lengths and nucleotide compositions of the polymerization products. The results show that the extent of polymerization, the degree of heteropolymerization vs. homopolymerization, and the composition of the polymeric products all vary among the different nucleotides and depend upon which nucleotides and how many different nucleotides are present in the mixture.

  5. The impact of quality control in RNA-seq experiments

    NASA Astrophysics Data System (ADS)

    Merino, Gabriela A.; Fresno, Cristóbal; Netto, Frederico; Dias Netto, Emmanuel; Pratto, Laura; Fernández, Elmer A.

    2016-04-01

    High throughput mRNA sample sequencing, known as RNA-seq, is as a powerful approach to detect differentially expressed genes starting from millions of short sequence reads. Although several workflows have been proposed to analyze RNA-seq data, the experiment quality control as a whole is not usually considered, thus potentially biasing the results and/or causing information lost. Experiment quality control refers to the analysis of the experiment as a whole, prior to any analysis. It not only inspects the presence of technical effects, but also if general biological assumptions are fulfilled. In this sense, multivariate approaches are crucial for this task. Here, a multivariate approach for quality control in RNA-seq experiments is proposed. This approach uses simple and yet effective well-known statistical methodologies. In particular, Principal Component Analysis was successfully applied over real data to detect and remove outlier samples. In addition, traditional multivariate exploration tools were applied in order to asses several controls that can help to ensure the results quality. Based on differential expression and functional enrichment analysis, here is demonstrated that the information retrieval is significantly enhanced through experiment quality control. Results show that the proposed multivariate approach increases the information obtained from RNA-seq data after outlier samples removal.

  6. Abiotic production of iodine molecules in irradiated ice

    NASA Astrophysics Data System (ADS)

    Choi, Wonyong; Kim, Kitae; Yabushita, Akihiro

    2015-04-01

    Reactive halogen species play an important role in Earth's environmental systems. Iodine compounds are related to ozone depletion event (ODE) during Antarctic spring, formation of CCN (cloud condensation nuclei), and controlling the atmospheric oxidizing capacity. However, the processes and mechanisms for abiotic formation of iodine compounds in polar region are still unclear. Although the chemical reactions taking place in ice are greatly different from those in aquatic environment, reaction processes of halogens in frozen condition have rarely studied compared to those in water. In this study, we investigated iodide oxidation to form triiodide (I3-) in ice phase under UV irradiation ( λ > 300 nm) and dark condition. The production of I3- through iodide oxidation, which is negligible in aqueous solution, was significantly accelerated in ice phase even in the absence of UV irradiation. The following release of gaseous iodine molecule (I2) to the atmosphere was also monitored by cavity ring-down spectroscopy (CRDS). We speculate that the markedly enhanced iodide oxidation in polycrystalline ice is due to the freeze concentration of iodides, protons, and dissolved oxygen in the ice crystal grain boundaries. The experiments conducted under ambient solar radiation of the Antarctic region (King George Island, 62°13'S 58°47'W, sea level) also confirmed that the generation of I3- via iodide oxidation process is enhanced when iodide is trapped in ice. The observed intrinsic oxidative transformation of iodide to generate I3-(aq) and I2(g) in frozen environment suggests a previously unknown pathway for the substantial release of reactive iodine species to the atmosphere.

  7. Salicylic acid-induced abiotic stress tolerance and underlying mechanisms in plants

    PubMed Central

    Khan, M. Iqbal R.; Fatma, Mehar; Per, Tasir S.; Anjum, Naser A.; Khan, Nafees A.

    2015-01-01

    Abiotic stresses (such as metals/metalloids, salinity, ozone, UV-B radiation, extreme temperatures, and drought) are among the most challenging threats to agricultural system and economic yield of crop plants. These stresses (in isolation and/or combination) induce numerous adverse effects in plants, impair biochemical/physiological and molecular processes, and eventually cause severe reductions in plant growth, development and overall productivity. Phytohormones have been recognized as a strong tool for sustainably alleviating adverse effects of abiotic stresses in crop plants. In particular, the significance of salicylic acid (SA) has been increasingly recognized in improved plant abiotic stress-tolerance via SA-mediated control of major plant-metabolic processes. However, the basic biochemical/physiological and molecular mechanisms that potentially underpin SA-induced plant-tolerance to major abiotic stresses remain least discussed. Based on recent reports, this paper: (a) overviews historical background and biosynthesis of SA under both optimal and stressful environments in plants; (b) critically appraises the role of SA in plants exposed to major abiotic stresses; (c) cross-talks potential mechanisms potentially governing SA-induced plant abiotic stress-tolerance; and finally (d) briefly highlights major aspects so far unexplored in the current context. PMID:26175738

  8. Tank Pressure Control Experiment/thermal Phenomena (TPCE/TP)

    NASA Technical Reports Server (NTRS)

    Hasan, M. M.; Knoll, R. H.

    1992-01-01

    The 'Tank Pressure Control Experiment/Thermal Phenomena (TPCE/TP)' is a reflight of the tank pressure control experiment (TPCE), flown on STS-43 in a standard Get-Away Special (GAS) container in August 1991. The TPCE obtained extensive video and digital data of the jet induced mixing process in a partially filled tank in low gravity environments. It also provided limited data on the thermal processes involved. The primary objective of the reflight of TPCE is to investigate experimentally the phenomena of liquid superheating and pool nucleate boiling at very low heat fluxes in a long duration low gravity environment. The findings of this experiment will be of direct relevance to space based subcritical cryogenic fluid system design and operation. Experiment hardware and results from the first TPCE are described in outline and graphic form.

  9. Low-Cost Undergraduate Control Systems Experiments Using Microcontroller-Based Control of a DC Motor

    ERIC Educational Resources Information Center

    Gunasekaran, M.; Potluri, R.

    2012-01-01

    This paper presents low-cost experiments for a control systems laboratory module that is worth one and a third credits. The experiments are organized around the microcontroller-based control of a permanent magnet dc motor. The experimental setups were built in-house. Except for the operating system, the software used is primarily freeware or free…

  10. Shock Wave / Boundary Layer Interaction Experiment on Control Surface

    DTIC Science & Technology

    2007-06-01

    like ice cubes do in water. When heat is added to the water/ ice mixture, ice melts , but the temperature of the mixture remains at 0ºC until all the ice ...ramp will likely experience similar oscillations (Figure 1). Various other groups have also performed numerical [9][10] and experimental [11][12][13... experimental data from all payloads in addition to some “housekeeping” signals. It also sends experiment control signals to the payloads. The

  11. Delayed feedback control experiments on some flexible structures

    NASA Astrophysics Data System (ADS)

    Cai, Guo-Ping; Chen, Long-Xiang

    2010-12-01

    In recent decades, studies on delayed system dynamics have attracted increasing attention and advances have been achieved in stability, nonlinearity, delay identification, delay elimination and application. However, most of the existing work is on the theoretical basis and little is on the experiment. This paper presents our experimental studies on delayed feedback control conducted in recent years with the focus on the discussion of a DSP-based delayed experiment system. Some phenomena in our delay experiments are discussed and a few topics of interest for further research are brought forward.

  12. Thermal control surfaces on the MSFC LDEF experiments

    NASA Technical Reports Server (NTRS)

    Wilkes, Donald R.; Whitaker, Ann F.; Zwiener, James M.; Linton, Roger C.; Shular, David; Peters, Palmer N.; Gregory, John C.

    1992-01-01

    There were five Marshall Space Flight Center (MSFC) experiments on the LDEF. Each of those experiments carried thermal control surfaces either as test samples or as operational surfaces. These materials experienced varying degrees of mechanical and optical damage. Some materials were virtually unchanged by the extended exposure while others suffered extensive degradation. The synergistic effects due to the constituents of the space environment are evident in the diversity of these material changes. The sample complement for the MSFC experiments is described along with results of the continuing analyses efforts.

  13. Abiotic Bromination of Soil Organic Matter.

    PubMed

    Leri, Alessandra C; Ravel, Bruce

    2015-11-17

    Biogeochemical transformations of plant-derived soil organic matter (SOM) involve complex abiotic and microbially mediated reactions. One such reaction is halogenation, which occurs naturally in the soil environment and has been associated with enzymatic activity of decomposer organisms. Building on a recent finding that naturally produced organobromine is ubiquitous in SOM, we hypothesized that inorganic bromide could be subject to abiotic oxidations resulting in bromination of SOM. Through lab-based degradation treatments of plant material and soil humus, we have shown that abiotic bromination of particulate organic matter occurs in the presence of a range of inorganic oxidants, including hydrogen peroxide and assorted forms of ferric iron, producing both aliphatic and aromatic forms of organobromine. Bromination of oak and pine litter is limited primarily by bromide concentration. Fresh plant material is more susceptible to bromination than decayed litter and soil humus, due to a labile pool of mainly aliphatic compounds that break down during early stages of SOM formation. As the first evidence of abiotic bromination of particulate SOM, this study identifies a mechanistic source of the natural organobromine in humic substances and the soil organic horizon. Formation of organobromine through oxidative treatments of plant material also provides insights into the relative stability of aromatic and aliphatic components of SOM.

  14. Experiment Study on Fuzzy Vibration Control of Solar Panel

    NASA Astrophysics Data System (ADS)

    Li, Dongxu X.; Xu, Rui; Jiang, Jiangjian P.

    Some flexible appendages of spacecraft are cantilever plate structures, such as solar panels. These structures usually have very low damping ratios, high dimensional order, low modal frequencies and parameter uncertainties in dynamics. Their unwanted vibrations will be caused unavoidably, and harmful to the spacecraft. To solve this problem, the dynamic equations of the solar panel with piezoelectric patches are derived, and an accelerometer based fuzzy controller is designed. In order to verify the effectiveness of the vibration control algorithms, experiment research was conducted on a piezoelectric adaptive composite honeycomb cantilever panel. The experiment results demonstrate that the accelerometer-based fuzzy vibration control method can suppress the vibration of the solar panel effectively, the first bending mode damping ratio of the controlled system increase to 1.64%, and that is 3.56 times of the uncontrolled system.

  15. Experiments on Socio-Technical Systems: The Problem of Control.

    PubMed

    Kroes, Peter

    2016-06-01

    My aim is to question whether the introduction of new technologies in society may be considered to be genuine experiments. I will argue that they are not, at least not in the sense in which the notion of experiment is being used in the natural and social sciences. If the introduction of a new technology in society is interpreted as an experiment, then we are dealing with a notion of experiment that differs in an important respect from the notion of experiment as used in the natural and social sciences. This difference shows itself most prominently when the functioning of the new technological system is not only dependent on technological hardware but also on social 'software', that is, on social institutions such as appropriate laws, and actions of operators of the new technological system. In those cases we are not dealing with 'simply' the introduction of a new technology, but with the introduction of a new socio-technical system. I will argue that if the introduction of a new socio-technical system is considered to be an experiment, then the relation between the experimenter and the system on which the experiment is performed differs significantly from the relation in traditional experiments in the natural and social sciences. In the latter experiments it is assumed that the experimenter is not part of the experimental system and is able to intervene in and control the experimental system from the outside. With regard to the introduction of new socio-technical systems the idea that there is an experimenter outside the socio-technical system who intervenes in and controls that system becomes problematic. From that perspective we are dealing with a different kind of experiment.

  16. Strike Point Control for the National Spherical Torus Experiment (NSTX)

    SciTech Connect

    Kolemen, E.; Gates, D. A.; Rowley, C. W.; Kasdin, N. J.; Kallman, J.; Gerhardt, S.; Soukhanovskii, V.; Mueller, D.

    2010-07-09

    This paper presents the first control algorithm for the inner and outer strike point position for a Spherical Torus (ST) fusion experiment and the performance analysis of the controller. A liquid lithium divertor (LLD) will be installed on NSTX which is believed to provide better pumping than lithium coatings on carbon PFCs. The shape of the plasma dictates the pumping rate of the lithium by channeling the plasma to LLD, where strike point location is the most important shape parameter. Simulations show that the density reduction depends on the proximity of strike point to LLD. Experiments were performed to study the dynamics of the strike point, design a new controller to change the location of the strike point to desired location and stabilize it. The most effective PF coils in changing inner and outer strike points were identified using equilibrium code. The PF coil inputs were changed in a step fashion between various set points and the step response of the strike point position was obtained. From the analysis of the step responses, PID controllers for the strike points were obtained and the controller was tuned experimentally for better performance. The strike controller was extended to include the outer-strike point on the inner plate to accommodate the desired low outer-strike points for the experiment with the aim of achieving "snowflake" divertor configuration in NSTX.

  17. Evaluation of a Neuromechanical Walking Control Model Using Disturbance Experiments

    PubMed Central

    Song, Seungmoon; Geyer, Hartmut

    2017-01-01

    Neuromechanical simulations have been used to study the spinal control of human locomotion which involves complex mechanical dynamics. So far, most neuromechanical simulation studies have focused on demonstrating the capability of a proposed control model in generating normal walking. As many of these models with competing control hypotheses can generate human-like normal walking behaviors, a more in-depth evaluation is required. Here, we conduct the more in-depth evaluation on a spinal-reflex-based control model using five representative gait disturbances, ranging from electrical stimulation to mechanical perturbation at individual leg joints and at the whole body. The immediate changes in muscle activations of the model are compared to those of humans across different gait phases and disturbance magnitudes. Remarkably similar response trends for the majority of investigated muscles and experimental conditions reinforce the plausibility of the reflex circuits of the model. However, the model's responses lack in amplitude for two experiments with whole body disturbances suggesting that in these cases the proposed reflex circuits need to be amplified by additional control structures such as location-specific cutaneous reflexes. A model that captures these selective amplifications would be able to explain both steady and reactive spinal control of human locomotion. Neuromechanical simulations that investigate hypothesized control models are complementary to gait experiments in better understanding the control of human locomotion. PMID:28381996

  18. Hydrothermal organic synthesis experiments

    NASA Technical Reports Server (NTRS)

    Shock, Everett L.

    1992-01-01

    Ways in which heat is useful in organic synthesis experiments are described, and experiments on the hydrothermal destruction and synthesis of organic compounds are discussed. It is pointed out that, if heat can overcome kinetic barriers to the formation of metastable states from reduced or oxidized starting materials, abiotic synthesis under hydrothermal conditions is a distinct possibility. However, carefully controlled experiments which replicate the descriptive variables of natural hydrothermal systems have not yet been conducted with the aim of testing the hypothesis of hydrothermal organic systems.

  19. Unconscious Modulation of the Conscious Experience of Voluntary Control

    ERIC Educational Resources Information Center

    Linser, Katrin; Goschke, Thomas

    2007-01-01

    How does the brain generate our experience of being in control over our actions and their effects? Here, we argue that the perception of events as self-caused emerges from a comparison between anticipated and actual action-effects: if the representation of an event that follows an action is activated before the action, the event is experienced as…

  20. The Run Control system of the NA62 experiment

    NASA Astrophysics Data System (ADS)

    Lurkin, Nicolas

    2017-01-01

    The NA62 experiment at the CERN SPS aims at measuring the ultra-rare decay with 10% accuracy. This can be achieved by detecting about 100 Standard Model events with 10% background in 2-3 years of data taking. The experiment consists of a large number of subsystems dedicated to the detection of the incoming kaon and outgoing pion and also focusing on particle identification and vetoing capabilities. Run Control has been designed to link their trigger and data acquisition systems in a single central application easily controllable by educated but non-expert operators. The application has been continuously evolving over time, integrating new equipments and taking into account requirements and feedback from operation. Future development includes a more automatized system integrating the knowledge acquired during the operation of the experiment.

  1. Performance of active vibration control technology: the ACTEX flight experiments

    NASA Astrophysics Data System (ADS)

    Nye, T. W.; Manning, R. A.; Qassim, K.

    1999-12-01

    This paper discusses the development and results of two intelligent structures space-flight experiments, each of which could affect architecture designs of future spacecraft. The first, the advanced controls technology experiment I (ACTEX I), is a variable stiffness tripod structure riding as a secondary payload on a classified spacecraft. It has been operating well past its expected life since becoming operational in 1996. Over 60 on-orbit experiments have been run on the ACTEX I flight experiment. These experiments form the basis for in-space controller design problems and for concluding lifetime/reliability data on the active control components. Transfer functions taken during the life of ACTEX I have shown consistent predictability and stability in structural behavior, including consistency with those measurements taken on the ground prior to a three year storage period and the launch event. ACTEX I can change its modal characteristics by employing its dynamic change mechanism that varies preloads in portions of its structure. Active control experiments have demonstrated maximum vibration reductions of 29 dB and 16 dB in the first two variable modes of the system, while operating over a remarkable on-orbit temperature range of -80 °C to 129 °C. The second experiment, ACTEX II, was successfully designed, ground-tested, and integrated on an experimental Department of Defense satellite prior to its loss during a launch vehicle failure in 1995. ACTEX II also had variable modal behavior by virtue of a two-axis gimbal and added challenges of structural flexibility by being a large deployable appendage. Although the loss of ACTEX II did not provide space environment experience, ground testing resulted in space qualifying the hardware and demonstrated 21 dB, 14 dB, and 8 dB reductions in amplitude of the first three primary structural modes. ACTEX II could use either active and/or passive techniques to affect vibration suppression. Both experiments trailblazed

  2. [Tobacco control: an intersectorial experience in Tunja (Colombia)].

    PubMed

    Panader-Torres, Adriana; Agudelo-Cely, Nancy Aurora; Bolívar-Suárez, Yolima; Cárdenas-Cárdenas, Luz Mery

    2014-01-01

    Tobacco control in Colombia is regulated by Law 1335 of 2009. The implementation and monitoring of the provisions of this law require strengthening of intersectorial work at the local level. This field note presents an intersectorial work experience that was carried out in the municipality of Tunja (Colombia) to improve tobacco control. The Respirarte Group was established. This group consists of an intersectorial team composed of 15 institutions. The Respirarte Group achieved the following political and community actions: signing of an agreement on tobacco control by government actors, expedition of a local decree to comply with Law 1335 in the municipality, provision of information and communication, and social mobilization and monitoring. This experience serves as a national and international reference and its lessons could be used in the approach to other public health problems.

  3. Apollo experience report: Lunar module environmental control subsystem

    NASA Technical Reports Server (NTRS)

    Gillen, R. J.; Brady, J. C.; Collier, F.

    1972-01-01

    A functional description of the environmental control subsystem is presented. Development, tests, checkout, and flight experiences of the subsystem are discussed; and the design fabrication, and operational difficulties associated with the various components and subassemblies are recorded. Detailed information is related concerning design changes made to, and problems encountered with, the various elements of the subsystem, such as the thermal control water sublimator, the carbon dioxide sensing and control units, and the water section. The problems associated with water sterilization, water/glycol formulation, and materials compatibility are discussed. The corrective actions taken are described with the expection that this information may be of value for future subsystems. Although the main experiences described are problem oriented, the subsystem has generally performed satisfactorily in flight.

  4. Slew maneuvers of Spacecraft Control Laboratory Experiment (SCOLE)

    NASA Technical Reports Server (NTRS)

    Kakad, Yogendra P.

    1992-01-01

    This is the final report on the dynamics and control of slew maneuvers of the Spacecraft Control Laboratory Experiment (SCOLE) test facility. The report documents the basic dynamical equation derivations for an arbitrary large angle slew maneuver as well as the basic decentralized slew maneuver control algorithm. The set of dynamical equations incorporate rigid body slew maneuver and three dimensional vibrations of the complete assembly comprising the rigid shuttle, the flexible beam, and the reflector with an offset mass. The analysis also includes kinematic nonlinearities of the entire assembly during the maneuver and the dynamics of the interactions between the rigid shuttle and the flexible appendage. The equations are simplified and evaluated numerically to include the first ten flexible modes to yield a model for designing control systems to perform slew maneuvers. The control problem incorporates the nonlinear dynamical equations and is expressed in terms of a two point boundary value problem.

  5. Experience with synchronous and asynchronous digital control systems

    NASA Technical Reports Server (NTRS)

    Regenie, V. A.; Chacon, C. V.; Lock, W. P.

    1986-01-01

    Flight control systems have undergone a revolution since the days of simple mechanical linkages; presently the most advanced systems are full-authority, full-time digital systems controlling unstable aircraft. With the use of advanced control systems, the aerodynamic design can incorporate features that allow greater performance and fuel savings, as can be seen on the new Airbus design and advanced tactical fighter concepts. These advanced aircraft will be and are relying on the flight control system to provide the stability and handling qualities required for safe flight and to allow the pilot to control the aircraft. Various design philosophies have been proposed and followed to investigate system architectures for these advanced flight control systems. One major area of discussion is whether a multichannel digital control system should be synchronous or asynchronous. This paper addressed the flight experience at the Dryden Flight Research Facility of NASA's Ames Research Center with both synchronous and asynchronous digital flight control systems. Four different flight control systems are evaluated against criteria such as software reliability, cost increases, and schedule delays.

  6. Enhancing Schistosomiasis Control Strategy for Zimbabwe: Building on Past Experiences

    PubMed Central

    Chimbari, Moses J.

    2012-01-01

    Schistosoma haematobium and Schistosoma mansoni are prevalent in Zimbabwe to levels that make schistosomiasis a public health problem. Following three national surveys to map the disease prevalence, a national policy on control of schistosomiasis and soil transmitted helminths is being developed. This paper reviews the experiences that Zimbabwe has in the area of schistosomiasis control with a view to influence policy. A case study approach to highlight key experiences and outcomes was adopted. The benefits derived from intersectoral collaboration that led to the development of a model irrigation scheme that incorporates schistosomiasis control measures are highlighted. Similarly, the benefits of using plant molluscicides and fish and duck biological agents (Sargochromis codringtonii and Cairina moschata) are highlighted. Emphasis was also placed on the importance of utilizing locally developed water and sanitation technologies and the critical human resource base in the area of schistosomiasis developed over years. After synthesis of the case studies presented, it was concluded that while there is a need to follow the WHO recommended guidelines for schistosomiasis control it is important to develop a control strategy that is informed by work already done in the country. The importance of having a policy and local guidelines for schistosomiasis control is emphasized. PMID:22655171

  7. Field experiments using SPEAR: a speech control system for UGVs

    NASA Astrophysics Data System (ADS)

    Chhatpar, Siddharth R.; Blanco, Chris; Czerniak, Jeffrey; Hoffman, Orin; Juneja, Amit; Pruthi, Tarun; Liu, Dongqing; Karlsen, Robert; Brown, Jonathan

    2009-05-01

    This paper reports on a Field Experiment carried out by the Human Research and Engineering Directorate at Ft. Benning to evaluate the efficacy of using speech to control an Unmanned Ground Vehicle (UGV) concurrently with a handcontroller. The SPEAR system, developed by Think-A-Move, provides speech-control of UGVs. The system picks up user-speech in the ear canal with an in-ear microphone. This property allows it to work efficiently in high-noise environments, where traditional speech systems, employing external microphones, fail. It has been integrated with an iRobot PackBot 510 with EOD kit. The integrated system allows the hand-controller to be supplemented with speech for concurrent control. At Ft. Benning, the integrated system was tested by soldiers from the Officer Candidate School. The Experiment had dual focus: 1) Quantitative measurement of the time taken to complete each station and the cognitive load on users; 2) Qualitative evaluation of ease-of-use and ergonomics through soldier-feedback. Also of significant benefit to Think-A-Move was soldier-feedback on the speech-command vocabulary employed: What spoken commands are intuitive, and how the commands should be executed, e.g., limited-motion vs. unlimited-motion commands. Overall results from the Experiment are reported in the paper.

  8. Control and monitoring of oxygen fugacity in piston cylinder experiments

    NASA Astrophysics Data System (ADS)

    Matjuschkin, Vladimir; Brooker, Richard A.; Tattitch, Brian; Blundy, Jon D.; Stamper, Charlotte C.

    2015-01-01

    We present a newly developed capsule design that resolves some common problems associated with the monitoring and control of oxygen fugacity ( fO2) in high-pressure piston cylinder experiments. The new fO2 control assembly consists of an AuPd outer capsule enclosing two inner capsules: one of AuPd capsule containing the experimental charge (including some water), and the other of Pt containing a solid oxygen buffer plus water. The inner capsules are separated by crushable alumina. The outer capsule is surrounded by a Pyrex sleeve to simultaneously minimise hydrogen loss from the cell and carbon infiltration from the graphite furnace. Controlled fO2 experiments using this cell design were carried out at 1.0 GPa and 1,000 °C. We used NiPd, CoPd and (Ni, Mg)O fO2 sensors, whose pressure sensitivity is well calibrated, to monitor the redox states achieved in experiments buffered by Re-ReO2, Ni-NiO and Co-CoO, respectively. Results for the fO2 sensors are in good agreement with the intended fO2 established by the buffer, demonstrating excellent control for durations of 24-48 h, with uncertainties less than ± 0.3 log bar units of fO2.

  9. An active thermal control surfaces experiment. [spacecraft temperature determination

    NASA Technical Reports Server (NTRS)

    Wilkes, D. R.; Brown, M. J.

    1979-01-01

    An active flight experiment is described that has the objectives to determine the effects of the low earth natural environment and the Shuttle induced environment on selected thermal control and optical surfaces. The optical and thermal properties of test samples will be measured in-situ using an integrating sphere reflectrometer and using calorimetric methods. This experiment has been selected for the Long Duration Exposure Facility (LDEF) flight which will be carried to orbit by the NASA Space Shuttle. The LDEF will remain in orbit to be picked up by a later Shuttle mission and returned for postflight evaluation.

  10. EURECA mission control experience and messages for the future

    NASA Technical Reports Server (NTRS)

    Huebner, H.; Ferri, P.; Wimmer, W.

    1994-01-01

    EURECA is a retrievable space platform which can perform multi-disciplinary scientific and technological experiments in a Low Earth Orbit for a typical mission duration of six to twelve months. It is deployed and retrieved by the NASA Space Shuttle and is designed to support up to five flights. The first mission started at the end of July 1992 and was successfully completed with the retrieval in June 1993. The operations concept and the ground segment for the first EURECA mission are briefly introduced. The experiences in the preparation and the conduction of the mission from the flight control team point of view are described.

  11. Identification of genes involved in the response of Arabidopsis to simultaneous biotic and abiotic stresses.

    PubMed

    Atkinson, Nicky J; Lilley, Catherine J; Urwin, Peter E

    2013-08-01

    In field conditions, plants may experience numerous environmental stresses at any one time. Research suggests that the plant response to multiple stresses is different from that for individual stresses, producing nonadditive effects. In particular, the molecular signaling pathways controlling biotic and abiotic stress responses may interact and antagonize one another. The transcriptome response of Arabidopsis (Arabidopsis thaliana) to concurrent water deficit (abiotic stress) and infection with the plant-parasitic nematode Heterodera schachtii (biotic stress) was analyzed by microarray. A unique program of gene expression was activated in response to a combination of water deficit and nematode stress, with 50 specifically multiple-stress-regulated genes. Candidate genes with potential roles in controlling the response to multiple stresses were selected and functionally characterized. RAPID ALKALINIZATION FACTOR-LIKE8 (AtRALFL8) was induced in roots by joint stresses but conferred susceptibility to drought stress and nematode infection when overexpressed. Constitutively expressing plants had stunted root systems and extended root hairs. Plants may produce signal peptides such as AtRALFL8 to induce cell wall remodeling in response to multiple stresses. The methionine homeostasis gene METHIONINE GAMMA LYASE (AtMGL) was up-regulated by dual stress in leaves, conferring resistance to nematodes when overexpressed. It may regulate methionine metabolism under conditions of multiple stresses. AZELAIC ACID INDUCED1 (AZI1), involved in defense priming in systemic plant immunity, was down-regulated in leaves by joint stress and conferred drought susceptibility when overexpressed, potentially as part of abscisic acid-induced repression of pathogen response genes. The results highlight the complex nature of multiple stress responses and confirm the importance of studying plant stress factors in combination.

  12. Notification: Notification Memo for Evaluation of Management Controls for Alternative Asbestos Control Method Experiments

    EPA Pesticide Factsheets

    Project #OPE-FY12-0011, February 27, 2012. This memorandum is to notify you that the Office of Inspector General (OIG) is initiating an evaluation on the Alternative Asbestos Control Method (AACM) experiments.

  13. Stable Isotope Systematics of Abiotic Nitrite Reduction Coupled with Anaerobic Iron Oxidation: The Role of Reduced Clays and Fe-bearing Minerals

    NASA Astrophysics Data System (ADS)

    Grabb, K. C.; Buchwald, C.; Hansel, C. M.; Wankel, S. D.

    2014-12-01

    Under anaerobic conditions, it is widely assumed that nitrate (NO3-) and nitrite (NO2-) reduction is primarily the result of microbial respiration. However, it has also been shown that abiotic reduction of nitrate and nitrite by reduced iron (Fe(II)), whether mineral-bound or surface-associated, may also occur under certain environmentally relevant conditions. With a range of experimental conditions, we investigated the nitrogen and oxygen stable isotope systematics of abiotic nitrite reduction by Fe(II) in an effort to characterize biotic and abiotic processes in the environment. While homogenous reactions between NO2- and Fe(II) in artificial seawater showed little reduction, heterogeneous reactions involving Fe-containing minerals showed considerable nitrite loss. Specifically, rapid nitrite reduction was observed in experiments that included reduced clays (illite, Na-montmorillonite, and nontronite) and those that exhibited iron oxide formation (ferrihydrite, magnetite and/or green rust). While these iron oxides and clay minerals offer both a source of reduced iron in the mineral matrix as well as a surface for Fe(II) activation, control experiments with corundum as a non-Fe containing mineral surface showed little NO2- loss, implicating a more dominant role of structural Fe in the clays during nitrite reduction. The isotope effects for 15N and 18O (15ɛ and 18ɛ) ranged from 5 to 14‰ for 15ɛ and 5 to 17‰ for 18ɛ and were typically coupled such that 15ɛ ~ 18ɛ. Reactions below pH 7 were slower and the 18ɛ was affected by oxygen atom exchange with water. Although little data exist for comparison with the dual isotopes of microbial NO2- reduction, these data serve as a benchmark for evaluating the role of abiotic processes in N reduction, particularly in sediment systems low in organic carbon and high in iron.

  14. Using thermodynamics to assess biotic and abiotic impediments to root water uptake

    NASA Astrophysics Data System (ADS)

    Bechmann, Marcel; Hildebrandt, Anke; Kleidon, Axel

    2016-04-01

    Root water uptake has been the subject of extensive research, dealing with understanding the processes limiting transpiration and understanding strategies of plants to avoid water stress. Many of those studies use models of water flow from the soil through the plant into the atmosphere to learn about biotic and abiotic factors affecting plant water relations. One important question in this context is to identify those processes that are most limiting to water transport, and specifically whether these processes lie within the plant or the soil? Here, we propose to use a thermodynamic formulation of root water uptake to answer this question. The method allows us to separate the energy exported at the root collar into a sum of energy fluxes related to all processes along the flow path, notably including the effect of increasing water retention in drier soils. Evaluation of the several contributions allows us to identify and rank the processes by how much these impede water flow from the soil to the atmosphere. The application of this approach to a complex 3-dimensional root water uptake model reveals insights on the role of root versus soil resistances to limit water flow. We investigate the efficiency of root water uptake in an ensemble of root systems with varying root hydraulic properties. While root morphology is kept the same, root radial and axial resistances are artificially varied. Starting with entirely young systems (uptake roots, high radial, low axial conductance) we increasingly add older roots (transport roots, high axial, low radial conductance) to improve transport within root systems. This yields a range of root hydraulic architectures, where the extremes are limited either by radial uptake capacity or low capacity to transport water along the root system. We model root water uptake in this range of root systems with a 3-dimensional root water uptake model in two different soils, applying constant flux boundary conditions in a dry down experiment and

  15. Experiments on vibration control of a piezoelectric laminated paraboloidal shell

    NASA Astrophysics Data System (ADS)

    Yue, Honghao; Lu, Yifan; Deng, Zongquan; Tzou, Hornsen

    2017-01-01

    A paraboloidal shell plays a key role in aerospace and optical structural systems applied to large optical reflector, communications antenna, rocket fairing, missile radome, etc. Due to the complexity of analytical procedures, an experimental study of active vibration control of a piezoelectric laminated paraboloidal shell by positive position feedback is carried out. Sixteen PVDF patches are laminated inside and outside of the shell, in which eight of them are used as sensors and eight as actuators to control the vibration of the first two natural modes. Lower natural frequencies and vibration modes of the paraboloidal shell are obtained via the frequency response function analysis by Modal VIEW software. A mathematical model of the control system is formulated by means of parameter identification. The first shell mode is controlled as well as coupled the first and second modes based on the positive position feedback (PPF) algorithm. To minimize the control energy consumption in orbit, an adaptive modal control method is developed in this study by using the PPF in laboratory experiments. The control system collects vibration signals from the piezoelectric sensors to identify location(s) of the largest vibration amplitudes and then select the best two from eight PVDF actuators to apply control forces so that the modal vibration suppression could be accomplished adaptively and effectively.

  16. Instrument canister thermal control. [for Space Shuttle-borne experiments

    NASA Technical Reports Server (NTRS)

    Harwell, W.; Haslett, R.; Ollendorf, S.

    1977-01-01

    A transient thermal analysis and test of a thermal control canister is described. The 1 x 1 x 3 m canister provides a uniform thermal environment for shuttle instrument payloads requiring fine temperature control, the design goal being operation between 0 C and 20 C with a range of plus or minus 1 C at any selected set-point temperature. The canister side walls are isothermalized by a system of longitudinal and circumferential heat pipes rejecting heat through feedback controlled, variable conductance heat pipes to side mounted radiators. A breadboard model of two side walls and two radiators was tested in a thermal vacuum chamber. The breadboard was stable over a wide range of effective environments, experiment dissipations, and control point temperature levels.

  17. Coupled Abiotic-Biotic Degradation of Bisphenol A

    NASA Astrophysics Data System (ADS)

    Im, J.; Prevatte, C.; Campagna, S. R.; Loeffler, F.

    2014-12-01

    Bisphenol A (BPA) is a ubiquitous environmental contaminant with weak estrogenic activity. BPA is readily biodegradable with oxygen available, but is recalcitrant to microbial degradation under anoxic conditions. However, BPA is susceptible to abiotic transformation under anoxic conditions. To better understand the fate of BPA in anoxic environments, the kinetics of BPA transformation by manganese oxide (d-MnO2) were investigated. BPA was rapidly transformed by MnO2 with a pseudo-first-order rate constant of 0.413 min-1. NMR and LC-MS analyses identified 4-hydroxycumyl alcohol (HCA) as a major intermediate. Up to 64% of the initial amount of BPA was recovered as HCA within 5 min, but the conversion efficiency decreased with time, suggesting that HCA was further degraded by MnO2. Further experiments confirmed that HCA was also susceptible to transformation by MnO2, albeit at 5-fold lower rates than BPA transformation. Mass balance approaches suggested that HCA was the major BPA transformation intermediate, but other compounds may also be formed. The abiotic transformation of BPA by MnO2 was affected by pH, and 10-fold higher transformation rates were observed at pH 4.5 than at pH 10. Compared to BPA, HCA has a lower octanol-water partitioning coefficient (Log Kow) of 0.76 vs 2.76 for BPA and a higher aqueous solubility of 2.65 g L-1 vs 0.31 g L-1 for BPA, suggesting higher mobility of HCA in the environment. Microcosms established with freshwater sediment materials collected from four geographically distinct locations and amended with HCA demonstrated rapid HCA biodegradation under oxic, but not under anoxic conditions. These findings suggest that BPA is not inert under anoxic conditions and abiotic reactions with MnO2 generate HCA, which has increased mobility and is susceptible to aerobic degradation. Therefore, coupled abiotic-biotic processes can affect the fate and longevity of BPA in terrestrial environments.

  18. Anticipatory balance control is affected by loadless training experiences.

    PubMed

    Robert, G; Gueguen, N; Avogadro, P; Mouchnino, L

    2004-09-01

    The main purpose of this study was to identify whether a lot of sports training had any effect on the balance control associated with a leg movement. The nature of the training experience was also an important concern and we chose subject who had undergone specific training experience in absence of equilibrium constraints. To this end a comparison between control (untrained) subjects, triathletes and swimmers was designed to establish whether a general training in sports (triathletes) or a specific loadless training (swimmers), leads to differences in the balance control. A leg movement is preceded by a shift of the center of mass (CM) towards the supporting side to maintain equilibrium and forward to create the condition for progression. To provide an acceleration of the CM sideward and forward, an initial displacement of the center of pressure (CP) towards the moving limb and in posterior direction was performed. Interestingly, the lateral pressure onto the ground was greater increased in swimmers in both leg raising and obstacle avoidance tasks compared to the control group and/or triathletes whereas the backward CP shift in all group was the same. The initial control of the CM shift is very different in swimmers compared to triathletes and controls. The increased lateral pressure onto the ground in swimmers may be a result of a prolonged training in water. This suggests that prolonged training in the absence of equilibrium constraints has more of an effect on balance control than a prolonged general training. In addition, the lack of differences in the backward CP shift suggests that M/L and A/P controls support two functional goals: equilibrium maintenance and movement initiation.

  19. Apollo experience report: Command and service module environmental control system

    NASA Technical Reports Server (NTRS)

    Samonski, F. H., Jr.; Tucker, E. M.

    1972-01-01

    A comprehensive review is presented of the design philosophy of the Apollo environmental control system together with the development history of the total system and of selected components within the system. In particular, discussions are presented relative to the development history and to the problems associated with the equipment cooling coldplates, the evaporator and its electronic control system, and the space radiator system used for rejection of the spacecraft thermal loads. Apollo flight experience and operational difficulties associated with the spacecraft water system and the waste management system are discussed in detail to provide definition of the problem and the corrective action taken when applicable.

  20. Implementation of BN Control in the National Spherical Torus Experiment

    SciTech Connect

    Gerhardt, S; Bell, M G; Cropper, M; Gates, D A; Koleman, E; Lawson, J; Marsala, B; Menard, J E; Mueller, D

    2012-09-15

    We have designed and constructed a system for control of the normalized B in the National Spherical Torus Experiment [M. Ono, et al., Nuclear Fusion 40, 557 (2000)]. A PID operator is applied to the difference between the present value of B N (from realtime equilibrium reconstruction) and a time-dependent request, in order to calculate the required injected power. This injected power request is then turned into modulations of the neutral beams. The details of this algorithm are described, including the techniques used to develop the appropriate control gains. Example uses of the system are shown

  1. Experiments with a small behaviour controlled planetary rover

    NASA Technical Reports Server (NTRS)

    Miller, David P.; Desai, Rajiv S.; Gat, Erann; Ivlev, Robert; Loch, John

    1993-01-01

    A series of experiments that were performed on the Rocky 3 robot is described. Rocky 3 is a small autonomous rover capable of navigating through rough outdoor terrain to a predesignated area, searching that area for soft soil, acquiring a soil sample, and depositing the sample in a container at its home base. The robot is programmed according to a reactive behavior control paradigm using the ALFA programming language. This style of programming produces robust autonomous performance while requiring significantly less computational resources than more traditional mobile robot control systems. The code for Rocky 3 runs on an eight bit processor and uses about ten k of memory.

  2. Rotor experiments in controlled conditions continued: New Mexico

    NASA Astrophysics Data System (ADS)

    Boorsma, K.; Schepers, J. G.

    2016-09-01

    To validate and reduce the large uncertainty associated with rotor aerodynamic and acoustic models, there is a need for detailed force, noise and surrounding flow velocity measurements on wind turbines under controlled conditions. However, high quality wind tunnel campaigns on horizontal axis wind turbine models are scarce due to the large wind tunnel size needed and consequently high associated costs. To serve this purpose an experiment using the Mexico turbine was set-up in the large low speed facility of the DNW wind tunnel. An overview of the experiments is given including a selection of results. A comparison of calculations to measurements for design conditions shows a satisfactory agreement. In summary, after years of preparation, ECN and partners have performed very successful aerodynamic experiments in the largest wind tunnel in Europe. The comprehensive high quality database that has been obtained will be used in the international Mexnext consortium to further develop wind energy aerodynamic and acoustic modeling.

  3. Dynamics and control simulation of the Spacelab Experiment Pointing Mount

    NASA Technical Reports Server (NTRS)

    Marsh, E. L.; Ward, R. S.

    1977-01-01

    Computer simulations were developed to evaluate the performance of four Experiment Pointing Mounts (EPM) being considered for Spacelab experiments in the 1980-1990 time frame. The system modeled compromises a multibody system consisting of the shuttle, a mechanical isolation device, the EPM, celestial and inertial sensors, bearings, gimbal torque motors and associated nonlinearities, the experiment payload, and control and estimator algorithms. Each mount was subjected to a common disturbance (shuttle vernier thruster firing and man push off) and command (stellar pointing or solar raster scan) input. The fundamental limitation common to all mounts was found to be sensor noise. System dynamics and hardware nonlinearities have secondary effects on pointing performance for sufficiently high bandwidth.

  4. IRIS Controlled Source Seismic Experiments: Continental Structure, Instrumentation, and Education

    NASA Astrophysics Data System (ADS)

    Mooney, W. D.; Keller, G. R.

    2004-12-01

    The controlled-source seismology program of IRIS/PASSCAL has made major contributions to the study of continental structure and evolution. It has also undergone major developments in seismic instrumentation. The first PASSCAL experiments (1984/85) targeted the Basin and Range Province and the Ouachita orogenic belt. The Basin and Range study provided remarkably clear images of this thin, highly-extended crust, while the Ouachita experiment tested competing hypotheses for the deep structure of this Paleozoic orogen. However, both of these projects were limited by a lack of seismic instruments. The situation improved in the late 1980's with the benefit of a mixed array of 600 seismic recorders from the USGS, Stanford, and the Geological Survey of Canada. The resolution achieved with these instruments was revolutionary. Results include the imaging of such remarkable features as crustal-scale duplexes in the Brooks Range compressional orogen of northern Alaska, and of crustal "core complexes" in the extended crust of southwest Arizona. The 3-channel PASSCAL Jr. instrument was developed, leading to experiments in which ˜1000 instruments were deployed, including three-component recording. This complex mix of instruments served the community well for several years, but required large, complex instrument centers and lots of technical support. With input from PASSCAL and the international community, a newly designed, compact instrument (the Texan) was finalized in the spring of 1998, and the first 200 instruments was delivered to the Univ. of Texas-El Paso in late 1998. The present instrument pool of Texans exceeds 1,400 and these have been used on such projects as the high-resolution imaging of the Los Angeles and San Fernando basins (LARSE I and II experiments), where active thrust faults have been imaged. Controlled-source seismic experiments are now very numerous. During calendar year 2004 alone, portable Texan instruments have traveled from Venezuela to Denmark

  5. Abiotic Transformation Of Estrogens In Synthetic Municipal Wastewater: An Alternative For Treatment?

    EPA Science Inventory

    The abiotic transformation of estrogens, including estrone (E1), estradiol (E2), estriol (E3) and ethinylestradiol (EE2), in the presence of model vegetable matter was confirmed in this study. Batch experiments were performed to model the catalytic conversion of E1, E2, E3, and ...

  6. Controlled ecological life support system higher plant flight experiments

    NASA Technical Reports Server (NTRS)

    Tibbitts, T. W.; Wheeler, R. M.

    1984-01-01

    Requirements for spaceflight experments which involve higher plants were determined. The plants are studied for use in controlled ecological life support systems (CELSS). Two categories of research requirements are discussed: (1) the physical needs which include nutrient, water and gas exchange requirements; (2) the biological and physiological functions which affect plants in zero gravity environments. Physical problems studies are given the priority since they affect all biological experiments.

  7. The JPL telerobot operator control station: Operational experiences

    NASA Technical Reports Server (NTRS)

    Kan, Edwin P.

    1990-01-01

    The Operator Control Station of the JPL/NASA Telerobot Demonstration System provides an efficient man-machine interface for the performance of telerobot tasks. Its hardware and software have been designed with high flexibility. It provides a feedback-rich interactive environment in which the Operator performs teleoperation tasks, robotic tasks, and telerobotic tasks with ease. The to-date operational experiences of this system, particularly related to the Object Designate Process and the Voice Input/Output Process are discussed.

  8. Abiotic Racemization Kinetics of Amino Acids in Marine Sediments

    PubMed Central

    Steen, Andrew D.; Jørgensen, Bo Barker; Lomstein, Bente Aa.

    2013-01-01

    The ratios of d- versus l-amino acids can be used to infer the sources and composition of sedimentary organic matter. Such inferences, however, rely on knowing the rates at which amino acids in sedimentary organic matter racemize abiotically between the d- and the l-forms. Based on a heating experiment, we report kinetic parameters for racemization of aspartic acid, glutamic acid, serine, and alanine in bulk sediment from Aarhus Bay, Denmark, taken from the surface, 30 cm, and 340 cm depth below seafloor. Extrapolation to a typical cold deep sea sediment temperature of 3°C suggests racemization rate constants of 0.50×10−5–11×10−5 yr−1. These results can be used in conjunction with measurements of sediment age to predict the ratio of d:l amino acids due solely to abiotic racemization of the source material, deviations from which can indicate the abundance and turnover of active microbial populations. PMID:23951211

  9. Efficient Dissemination Control of Tele-Experiment Data

    NASA Astrophysics Data System (ADS)

    Perez-Vernet, M.

    2002-01-01

    Operations of space stations experiments raises new requirements for telescience approaches [1], [3]. To relieve astronauts from workload to control the experiments in orbit, it would be desirable to have them directly controlled from the prime investigator's computer on ground. On the other side the data acquired are to be transferred automatically to all the experimenters or possibly to a broader audience via inexpensive Internet links. This paper investigates the distributed information system approaches to establish such an infrastructure. Approaches based on Java servlets and applets in comparison to CGI, as well as Corba to deal with the inhomogeneous computer networks have been analysed. In particular are investigated for the different options. Other informatics features concern approaches to graphical user interfaces, as well as advanced methods in the field of virtual reality [2] and of haptic interfaces [4]. Quick dissemination of Here an example application [3] based on mobile robots has been implemented and tested for performance under varying Internet conditions. In[4] O. Rösch, K. Schilling, H. Roth. Haptic outreach activities the remotely controlled roversInterfaces for Remote Control of Mobile Robots, have presented to pupils and students as anproceedings 1st IFAC Conference on Telematics motivating introduction to space and computerApplications in Automation and Robitics, Pergamon systems.Press 2001, p.143-148. References [1] P. Backes, K. Tso, J. Norris, G. Tharp, R. Bonitz, K. M. Pérez Vernet, K. Schilling. "Virtual

  10. Biotic and abiotic factors predicting the global distribution and population density of an invasive large mammal

    PubMed Central

    Lewis, Jesse S.; Farnsworth, Matthew L.; Burdett, Chris L.; Theobald, David M.; Gray, Miranda; Miller, Ryan S.

    2017-01-01

    Biotic and abiotic factors are increasingly acknowledged to synergistically shape broad-scale species distributions. However, the relative importance of biotic and abiotic factors in predicting species distributions is unclear. In particular, biotic factors, such as predation and vegetation, including those resulting from anthropogenic land-use change, are underrepresented in species distribution modeling, but could improve model predictions. Using generalized linear models and model selection techniques, we used 129 estimates of population density of wild pigs (Sus scrofa) from 5 continents to evaluate the relative importance, magnitude, and direction of biotic and abiotic factors in predicting population density of an invasive large mammal with a global distribution. Incorporating diverse biotic factors, including agriculture, vegetation cover, and large carnivore richness, into species distribution modeling substantially improved model fit and predictions. Abiotic factors, including precipitation and potential evapotranspiration, were also important predictors. The predictive map of population density revealed wide-ranging potential for an invasive large mammal to expand its distribution globally. This information can be used to proactively create conservation/management plans to control future invasions. Our study demonstrates that the ongoing paradigm shift, which recognizes that both biotic and abiotic factors shape species distributions across broad scales, can be advanced by incorporating diverse biotic factors. PMID:28276519

  11. Development of electrical feedback controlled heat pipes and the advanced thermal control flight experiment

    NASA Technical Reports Server (NTRS)

    Bienert, W. B.

    1974-01-01

    The development and characteristics of electrical feedback controlled heat pipes (FCHP) are discussed. An analytical model was produced to describe the performance of the FCHP under steady state and transient conditions. An advanced thermal control flight experiment was designed to demonstrate the performance of the thermal control component in a space environment. The thermal control equipment was evaluated on the ATS-F satellite to provide performance data for the components and to act as a thermal control system which can be used to provide temperature stability of spacecraft components in future applications.

  12. Control of the ST7 Disturbance Reduction System Flight Experiment

    NASA Technical Reports Server (NTRS)

    Maghami, P. G.; Hsu, O. C,; ODonnell, J. R., Jr.

    2007-01-01

    The Space Technology 7 (ST7) experiment will perform an on-orbit system-level validation of two specific Disturbance Reduction System technologies: colloidal micronewton thrusters and drag-free control. The ST7 Disturbance Reduction System (DRS) is designed to maintain the spacecraft s position with respect to a free-floating test mass while limiting the residual accelerations of that test mass over the frequency range of 1 to 30 mHz. This paper presents the overall design and analysis of the spacecraft drag-free and attitude controllers, with particular attention given to its primary mission mode. These controllers close the loop between the drag-free sensors and the colloidal micronewton thrusters.

  13. The spacecraft control laboratory experiment optical attitude measurement system

    NASA Technical Reports Server (NTRS)

    Welch, Sharon S.; Montgomery, Raymond C.; Barsky, Michael F.

    1991-01-01

    A stereo camera tracking system was developed to provide a near real-time measure of the position and attitude of the Spacecraft COntrol Laboratory Experiment (SCOLE). The SCOLE is a mockup of the shuttle-like vehicle with an attached flexible mast and (simulated) antenna, and was designed to provide a laboratory environment for the verification and testing of control laws for large flexible spacecraft. Actuators and sensors located on the shuttle and antenna sense the states of the spacecraft and allow the position and attitude to be controlled. The stereo camera tracking system which was developed consists of two position sensitive detector cameras which sense the locations of small infrared LEDs attached to the surface of the shuttle. Information on shuttle position and attitude is provided in six degrees-of-freedom. The design of this optical system, calibration, and tracking algorithm are described. The performance of the system is evaluated for yaw only.

  14. Expression of the tetrahydrofolate-dependent nitric oxide synthase from the green alga Ostreococcus tauri increases tolerance to abiotic stresses and influences stomatal development in Arabidopsis.

    PubMed

    Foresi, Noelia; Mayta, Martín L; Lodeyro, Anabella F; Scuffi, Denise; Correa-Aragunde, Natalia; García-Mata, Carlos; Casalongué, Claudia; Carrillo, Néstor; Lamattina, Lorenzo

    2015-06-01

    Nitric oxide (NO) is a signaling molecule with diverse biological functions in plants. NO plays a crucial role in growth and development, from germination to senescence, and is also involved in plant responses to biotic and abiotic stresses. In animals, NO is synthesized by well-described nitric oxide synthase (NOS) enzymes. NOS activity has also been detected in higher plants, but no gene encoding an NOS protein, or the enzymes required for synthesis of tetrahydrobiopterin, an essential cofactor of mammalian NOS activity, have been identified so far. Recently, an NOS gene from the unicellular marine alga Ostreococcus tauri (OtNOS) has been discovered and characterized. Arabidopsis thaliana plants were transformed with OtNOS under the control of the inducible short promoter fragment (SPF) of the sunflower (Helianthus annuus) Hahb-4 gene, which responds to abiotic stresses and abscisic acid. Transgenic plants expressing OtNOS accumulated higher NO concentrations compared with siblings transformed with the empty vector, and displayed enhanced salt, drought and oxidative stress tolerance. Moreover, transgenic OtNOS lines exhibited increased stomatal development compared with plants transformed with the empty vector. Both in vitro and in vivo experiments indicate that OtNOS, unlike mammalian NOS, efficiently uses tetrahydrofolate as a cofactor in Arabidopsis plants. The modulation of NO production to alleviate abiotic stress disturbances in higher plants highlights the potential of genetic manipulation to influence NO metabolism as a tool to improve plant fitness under adverse growth conditions.

  15. Natural abiotic formation of oxalic acid in soils: results from aromatic model compounds and soil samples.

    PubMed

    Studenroth, Sabine; Huber, Stefan G; Kotte, Karsten; Schöler, Heinz F

    2013-02-05

    Oxalic acid is the smallest dicarboxylic acid and plays an important role in soil processes (e.g., mineral weathering and metal detoxification in plants). We have first proven its abiotic formation in soils and investigated natural abiotic degradation processes based on the oxidation of soil organic matter, enhanced by Fe(3+) and H(2)O(2) as hydroxyl radical suppliers. Experiments with the model compound catechol and further hydroxylated benzenes were performed to examine a common degradation pathway and to presume a general formation mechanism of oxalic acid. Two soil samples were tested for the release of oxalic acid and the potential effects of various soil parameters on oxalic acid formation. Additionally, the soil samples were treated with different soil sterilization methods to prove the oxalic acid formation under abiotic soil conditions. Different series of model experiments were conducted to determine a range of factors including Fe(3+), H(2)O(2), reaction time, pH, and chloride concentration on oxalic acid formation. Under certain conditions, catechol is degraded up to 65.6% to oxalic acid referring to carbon. In serial experiments with two soil samples, oxalic acid was produced, and the obtained results are suggestive of an abiotic degradation process. In conclusion, Fenton-like conditions with low Fe(3+) concentrations and an excess of H(2)O(2) as well as acidic conditions were required for an optimal oxalic acid formation. The presence of chloride reduced oxalic acid formation.

  16. Surface chemistry allows for abiotic precipitation of dolomite at low temperature

    PubMed Central

    Roberts, Jennifer A.; Kenward, Paul A.; Fowle, David A.; Goldstein, Robert H.; González, Luis A.; Moore, David S.

    2013-01-01

    Although the mineral dolomite is abundant in ancient low-temperature sedimentary systems, it is scarce in modern systems below 50 °C. Chemical mechanism(s) enhancing its formation remain an enigma because abiotic dolomite has been challenging to synthesize at low temperature in laboratory settings. Microbial enhancement of dolomite precipitation at low temperature has been reported; however, it is still unclear exactly how microorganisms influence reaction kinetics. Here we document the abiotic synthesis of low-temperature dolomite in laboratory experiments and constrain possible mechanisms for dolomite formation. Ancient and modern seawater solution compositions, with identical pH and pCO2, were used to precipitate an ordered, stoichiometric dolomite phase at 30 °C in as few as 20 d. Mg-rich phases nucleate exclusively on carboxylated polystyrene spheres along with calcite, whereas aragonite forms in solution via homogeneous nucleation. We infer that Mg ions are complexed and dewatered by surface-bound carboxyl groups, thus decreasing the energy required for carbonation. These results indicate that natural surfaces, including organic matter and microbial biomass, possessing a high density of carboxyl groups may be a mechanism by which ordered dolomite nuclei form. Although environments rich in organic matter may be of interest, our data suggest that sharp biogeochemical interfaces that promote microbial death, as well as those with high salinity may, in part, control carboxyl-group density on organic carbon surfaces, consistent with origin of dolomites from microbial biofilms, as well as hypersaline and mixing zone environments. PMID:23964124

  17. Tank Pressure Control Experiment on the Space Shuttle

    NASA Technical Reports Server (NTRS)

    1989-01-01

    The tank pressure control experiment is a demonstration of NASA intent to develop new technology for low-gravity management of the cryogenic fluids that will be required for future space systems. The experiment will use freon as the test fluid to measure the effects of jet-induced fluid mixing on storage tank pressure and will produce data on low-gravity mixing processes critical to the design of on-orbit cryogenic storage and resupply systems. Basic data on fluid motion and thermodynamics in low gravity is limited, but such data is critical to the development of space transfer vehicles and spacecraft resupply facilities. An in-space experiment is needed to obtain reliable data on fluid mixing and pressure control because none of the available microgravity test facilities provide a low enough gravity level for a sufficient duration to duplicate in-space flow patterns and thermal processes. Normal gravity tests do not represent the fluid behavior properly; drop-tower tests are limited in length of time available; aircraft low-gravity tests cannot provide the steady near-zero gravity level and long duration needed to study the subtle processes expected in space.

  18. Tank Pressure Control Experiment: Thermal Phenomena in Microgravity

    NASA Technical Reports Server (NTRS)

    Hasan, Mohammad M.; Lin, Chin S.; Knoll, Richard H.; Bentz, Michael D.

    1996-01-01

    The report presents the results of the flight experiment Tank Pressure Control Experiment/Thermal Phenomena (TPCE/TP) performed in the microgravity environment of the space shuttle. TPCE/TP, flown on the Space Transportation System STS-52, was a second flight of the Tank Pressure Control Experiment (TPCE). The experiment used Freon 113 at near saturation conditions. The test tank was filled with liquid to about 83% by volume. The experiment consisted of 21 tests. Each test generally started with a heating phase to increase the tank pressure and to develop temperature stratification in the fluid, followed by a fluid mixing phase for the tank pressure reduction and fluid temperature equilibration. The heating phase provided pool boiling data from large (relative to bubble sizes) heating surfaces (0.1046 m by 0.0742 m) at low heat fluxes (0.23 to 1.16 kW/sq m). The system pressure and the bulk liquid subcooling varied from 39 to 78 kPa and 1 to 3 C, respectively. The boiling process during the entire heating period, as well as the jet-induced mixing process for the first 2 min of the mixing period, was also recorded on video. The unique features of the experimental results are the sustainability of high liquid superheats for long periods and the occurrence of explosive boiling at low heat fluxes (0.86 to 1.1 kW/sq m). For a heat flux of 0.97 kW/sq m, a wall superheat of 17.9 C was attained in 10 min of heating. This superheat was followed by an explosive boiling accompanied by a pressure spike of about 38% of the tank pressure at the inception of boiling. However, at this heat flux the vapor blanketing the heating surface could not be sustained. Steady nucleate boiling continued after the explosive boiling. The jet-induced fluid mixing results were obtained for jet Reynolds numbers of 1900 to 8000 and Weber numbers of 0.2 to 6.5. Analyses of data from the two flight experiments (TPCE and TPCE/TP) and their comparison with the results obtained in drop tower experiments

  19. The MODE family of on-orbit experiments: The Middeck Active Control Experiment (MACE)

    NASA Technical Reports Server (NTRS)

    Crawley, Edward F.; Miller, David W.; Deluis, Javier; Waldman, Mel; Bicos, Andy

    1990-01-01

    A flight experiment entitled the Middeck Active Control Experiment (MACE), proposed by the Space Engineering Research Center (SERC) at the Massachusetts Institute of Technology, is described. This is the second in a family of flight experiments being developed at MIT. The first is the Middeck 0-Gravity Dynamics Experiment (MODE) which investigates the nonlinear behavior of contained fluids and truss structures in zero gravity. The objective of the MACE program is to investigate and validate the modeling of the dynamics of an actively controlled flexible, articulating, multibody platform free floating in zero gravity. A rationale and experimental approach for the program are presented. The rationale shows that on-orbit testing, coupled with ground testing and a strong analytical program, is necessary in order to fully understand both how flexibility of the platform affects the pointing problem, as well as how gravity perturbs this structural flexibility causing deviations between 1- and 0-gravity behavior. The experimental approach captures the essential physics of multibody platforms, by identifying the appropriate attributes, tests, and performance metrics of the test article and defines the tests required to successfully validate the analytical framework.

  20. The MODE family of on-orbit experiments: The Middeck Active Control Experiment (MACE)

    NASA Astrophysics Data System (ADS)

    Crawley, Edward F.; Miller, David W.; Deluis, Javier; Waldman, Mel; Bicos, Andy

    1990-12-01

    A flight experiment entitled the Middeck Active Control Experiment (MACE), proposed by the Space Engineering Research Center (SERC) at the Massachusetts Institute of Technology, is described. This is the second in a family of flight experiments being developed at MIT. The first is the Middeck 0-Gravity Dynamics Experiment (MODE) which investigates the nonlinear behavior of contained fluids and truss structures in zero gravity. The objective of the MACE program is to investigate and validate the modeling of the dynamics of an actively controlled flexible, articulating, multibody platform free floating in zero gravity. A rationale and experimental approach for the program are presented. The rationale shows that on-orbit testing, coupled with ground testing and a strong analytical program, is necessary in order to fully understand both how flexibility of the platform affects the pointing problem, as well as how gravity perturbs this structural flexibility causing deviations between 1- and 0-gravity behavior. The experimental approach captures the essential physics of multibody platforms, by identifying the appropriate attributes, tests, and performance metrics of the test article and defines the tests required to successfully validate the analytical framework.

  1. C-SIDE: The control-structure interaction demonstration experiment

    NASA Technical Reports Server (NTRS)

    Mohl, James B.; Davis, Hugh W.

    1993-01-01

    The Control-Structure Interaction Demonstration Experiment (C-SIDE) is sponsored by the Electro-Optics and Cryogenics Division of Ball Aerospace Systems Group. Our objective is to demonstrate methods of solution to structure control problems utilizing currently available hardware in a system that is an extension of our corporate experience. The larger space structures with which Ball has been associated are the SEASAT radar antenna, Shuttle Imaging Radar (SIR) -A, -B and -C antennas and the Radarsat spacecraft. The motivation for the C-SIDE configuration is to show that integration of active figure control in the radar's system-level design can relieve antenna mechanical design constraints. This presentation is primarily an introduction to the C-SIDE testbed. Its physical and functional layouts, and major components are described. The sensor is of special interest as it enables direct surface figure measurements from a remote location. The Remote Attitude Measurement System (RAMS) makes high-rate, unobtrusive measurements of many locations, several of which may be collocated easily with actuators. The control processor is a 386/25 executing a reduced order model-based algorithm with provision for residual mode filters to compensate for structure interaction. The actuators for the ground demonstration are non-contacting, linear force devices. Results presented illustrate some basic characteristics of control-structure interaction with this hardware. The testbed will be used for evaluation of current technologies and for research in several areas. A brief indication of the evolution of the C-SIDE is given at the conclusion.

  2. A structured architecture for advanced plasma control experiments

    SciTech Connect

    Penaflor, B.G.; Ferron, J.R.; Walker, M.L.

    1996-10-01

    Recent new and improved plasma control regimes have evolved from enhancements to the systems responsible for managing the plasma configuration on the DIII-D tokamak. The collection of hardware and software components designed for this purpose is known at DIII-D as the Plasma Control System or PCS. Several new user requirements have contributed to the rapid growth of the PCS. Experiments involving digital control of the plasma vertical position have resulted in the addition of new high performance processors to operate in real-time. Recent studies in plasma disruptions involving the use of neural network based software have resulted in an increase in the number of input diagnostic signals sampled. Better methods for estimating the plasma shape and position have brought about numerous software changes and the addition of several new code modules. Furthermore, requests for performing multivariable control and feedback on the current profile are continuing to add to the demands being placed on the PCS. To support all of these demands has required a structured yet flexible hardware and software architecture for maintaining existing capabilities and easily adding new ones. This architecture along with a general overview of the DIII-D Plasma Control System is described. In addition, the latest improvements to the PCS are presented.

  3. Temporal dynamics of biotic and abiotic drivers of litter decomposition.

    PubMed

    García-Palacios, Pablo; Shaw, E Ashley; Wall, Diana H; Hättenschwiler, Stephan

    2016-05-01

    Climate, litter quality and decomposers drive litter decomposition. However, little is known about whether their relative contribution changes at different decomposition stages. To fill this gap, we evaluated the relative importance of leaf litter polyphenols, decomposer communities and soil moisture for litter C and N loss at different stages throughout the decomposition process. Although both microbial and nematode communities regulated litter C and N loss in the early decomposition stages, soil moisture and legacy effects of initial differences in litter quality played a major role in the late stages of the process. Our results provide strong evidence for substantial shifts in how biotic and abiotic factors control litter C and N dynamics during decomposition. Taking into account such temporal dynamics will increase the predictive power of decomposition models that are currently limited by a single-pool approach applying control variables uniformly to the entire decay process.

  4. Real-time experiment interface for biological control applications.

    PubMed

    Lin, Risa J; Bettencourt, Jonathan; Wha Ite, John; Christini, David J; Butera, Robert J

    2010-01-01

    The Real-time Experiment Interface (RTXI) is a fast and versatile real-time biological experimentation system based on Real-Time Linux. RTXI is open source and free, can be used with an extensive range of experimentation hardware, and can be run on Linux or Windows computers (when using the Live CD). RTXI is currently used extensively for two experiment types: dynamic patch clamp and closed-loop stimulation pattern control in neural and cardiac single cell electrophysiology. RTXI includes standard plug-ins for implementing commonly used electrophysiology protocols with synchronized stimulation, event detection, and online analysis. These and other user-contributed plug-ins can be found on the website (http://www.rtxi.org).

  5. MISSE Thermal Control Materials with Comparison to Previous Flight Experiments

    NASA Technical Reports Server (NTRS)

    Finckenor, Miria; Pippin, H. Gary; Frey, George

    2008-01-01

    Many different passive thermal control materials were flown as part of the Materials on International Space Station Experiment (MISSE), including inorganic coatings, anodized aluminum, and multi-layer insulation materials. These and other material samples were exposed to the low Earth orbital environment of atomic oxygen, ultraviolet radiation, thermal cycling, and hard vacuum, though atomic oxygen exposure was limited for some samples. Materials flown on MISSE-1 and MISSE-2 were exposed to the space environment for nearly four years. Materials flown on MISSE-3, MISSE-4, and MISSE-5 were exposed to the space environment for one year. Solar absorptance, infrared emittance, and mass measurements indicate the durability of these materials to withstand the space environment. Effects of short duration versus long duration exposure on ISS are explored, as well as comparable data from previous flight experiments, such as the Passive Optical Sample Assembly (POSA), Optical Properties Monitor (OPM), and Long Duration Exposure Facility (LDEF).

  6. Oxylipins and plant abiotic stress resistance.

    PubMed

    Savchenko, T V; Zastrijnaja, O M; Klimov, V V

    2014-04-01

    Oxylipins are signaling molecules formed enzymatically or spontaneously from unsaturated fatty acids in all aerobic organisms. Oxylipins regulate growth, development, and responses to environmental stimuli of organisms. The oxylipin biosynthesis pathway in plants includes a few parallel branches named after first enzyme of the corresponding branch as allene oxide synthase, hydroperoxide lyase, divinyl ether synthase, peroxygenase, epoxy alcohol synthase, and others in which various biologically active metabolites are produced. Oxylipins can be formed non-enzymatically as a result of oxygenation of fatty acids by free radicals and reactive oxygen species. Spontaneously formed oxylipins are called phytoprostanes. The role of oxylipins in biotic stress responses has been described in many published works. The role of oxylipins in plant adaptation to abiotic stress conditions is less studied; there is also obvious lack of available data compilation and analysis in this area of research. In this work we analyze data on oxylipins functions in plant adaptation to abiotic stress conditions, such as wounding, suboptimal light and temperature, dehydration and osmotic stress, and effects of ozone and heavy metals. Modern research articles elucidating the molecular mechanisms of oxylipins action by the methods of biochemistry, molecular biology, and genetics are reviewed here. Data on the role of oxylipins in stress signal transduction, stress-inducible gene expression regulation, and interaction of these metabolites with other signal transduction pathways in cells are described. In this review the general oxylipin-mediated mechanisms that help plants to adjust to a broad spectrum of stress factors are considered, followed by analysis of more specific responses regulated by oxylipins only under certain stress conditions. New approaches to improvement of plant resistance to abiotic stresses based on the induction of oxylipin-mediated processes are discussed.

  7. DASHBOARDS & CONTROL CHARTS EXPERIENCES IN IMPROVING SAFETY AT HANFORD WASHINGTON

    SciTech Connect

    PREVETTE, S.S.

    2006-02-27

    The aim of this paper is to demonstrate the integration of safety methodology, quality tools, leadership, and teamwork at Hanford and their significant positive impact on safe performance of work. Dashboards, Leading Indicators, Control charts, Pareto Charts, Dr. W. Edward Deming's Red Bead Experiment, and Dr. Deming's System of Profound Knowledge have been the principal tools and theory of an integrated management system. Coupled with involved leadership and teamwork, they have led to significant improvements in worker safety and protection, and environmental restoration at one of the nation's largest nuclear cleanup sites.

  8. Decompression experiments identify kinetic controls on explosive silicic eruptions

    USGS Publications Warehouse

    Mangan, M.T.; Sisson, T.W.; Hankins, W.B.

    2004-01-01

    Eruption intensity is largely controlled by decompression-induced release of water-rich gas dissolved in magma. It is not simply the amount of gas that dictates how forcefully magma is propelled upwards during an eruption, but also the rate of degassing, which is partly a function of the supersaturation pressure (??Pcritical) triggering gas bubble nucleation. High temperature and pressure decompression experiments using rhyolite and dacite melt reveal compositionally-dependent differences in the ??Pcritical of degassing that may explain why rhyolites have fueled some of the most explosive eruptions on record.

  9. Apollo experience report: Guidance and control systems. Lunar module stabilization and control system

    NASA Technical Reports Server (NTRS)

    Shelton, D. H.

    1975-01-01

    A brief functional description of the Apollo lunar module stabilization and control subsystem is presented. Subsystem requirements definition, design, development, test results, and flight experiences are discussed. Detailed discussions are presented of problems encountered and the resulting corrective actions taken during the course of assembly-level testing, integrated vehicle checkout and test, and mission operations. Although the main experiences described are problem oriented, the subsystem has performed satisfactorily in flight.

  10. Apollo experience report: Guidance and control systems. Engineering simulation program

    NASA Technical Reports Server (NTRS)

    Gilbert, D. W.

    1973-01-01

    The Apollo Program experience from early 1962 to July 1969 with respect to the engineering-simulation support and the problems encountered is summarized in this report. Engineering simulation in support of the Apollo guidance and control system is discussed in terms of design analysis and verification, certification of hardware in closed-loop operation, verification of hardware/software compatibility, and verification of both software and procedures for each mission. The magnitude, time, and cost of the engineering simulations are described with respect to hardware availability, NASA and contractor facilities (for verification of the command module, the lunar module, and the primary guidance, navigation, and control system), and scheduling and planning considerations. Recommendations are made regarding implementation of similar, large-scale simulations for future programs.

  11. Shuttle Orbiter Environmental Control and Life Support System - Flight experience

    NASA Technical Reports Server (NTRS)

    Winkler, H. E.

    1992-01-01

    This paper describes the overall design of the Shuttle Orbiter Environmental Control and Life Support System (ECLSS). The Orbiter ECLSS consists of six major subsystems which accomplish the functions of providing a habitable pressurized cabin atmosphere and removing gaseous contaminants, controlling the temperature of the cabin and vehicle components within acceptable ranges, providing fire detection and suppression capability, maintaining a supply of potable water, collecting and removing metabolic waste materials, and providing utilities and access for extravehicular activity. The operational experience is summarized for the 45 space flights accomplished to date during which the Orbiter ECLSS has been demonstrated to perform reliably, and has proved to have the flexibility to meet a variety of mission needs. Significant flight problems are described, along with the design or procedure changes which were implemented to resolve the problems.

  12. Operator experiences on working in screen-based control rooms

    SciTech Connect

    Salo, L.; Laarni, J.; Savioja, P.

    2006-07-01

    This paper introduces the results of two interview studies carried out in Finland in four conventional power plants and one nuclear power plant. The aim of the studies was to gather data on user experiences on the effects of control room modernization and digital control room technology on operator work Since the number of completed digitalization projects in nuclear power plants is small supplementary information was gathered by interviewing operators in conventional power plants. Our results suggest that even though the modernization processes have been success stories, they have created new challenges for operator personnel. Examples of these challenges are increased requirements for competence and collaboration, problems in trust calibration and development of awareness of the process state. Some major differences in the digitalization of human-system interfaces between conventional and nuclear power plants were discussed. (authors)

  13. How Life Experience Shapes Cognitive Control Strategies: The Case of Air Traffic Control Training

    PubMed Central

    Arbula, Sandra; Capizzi, Mariagrazia; Lombardo, Nicoletta; Vallesi, Antonino

    2016-01-01

    Although human flexible behavior relies on cognitive control, it would be implausible to assume that there is only one, general mode of cognitive control strategy adopted by all individuals. For instance, different reliance on proactive versus reactive control strategies could explain inter-individual variability. In particular, specific life experiences, like a highly demanding training for future Air Traffic Controllers (ATCs), could modulate cognitive control functions. A group of ATC trainees and a matched group of university students were tested longitudinally on task-switching and Stroop paradigms that allowed us to measure indices of cognitive control. The results showed that the ATCs, with respect to the control group, had substantially smaller mixing costs during long cue-target intervals (CTI) and a reduced Stroop interference effect. However, this advantage was present also prior to the training phase. Being more capable in managing multiple task sets and less distracted by interfering events suggests a more efficient selection and maintenance of task relevant information as an inherent characteristic of the ATC group, associated with proactive control. Critically, the training that the ATCs underwent improved their accuracy in general and reduced response time switching costs during short CTIs only. These results indicate a training-induced change in reactive control, which is described as a transient process in charge of stimulus-driven task detection and resolution. This experience-based enhancement of reactive control strategy denotes how cognitive control and executive functions in general can be shaped by real-life training and underlines the importance of experience in explaining inter-individual variability in cognitive functioning. PMID:27311017

  14. Abiotic stress growth conditions induce different responses in kernel iron concentration across genotypically distinct maize inbred varieties.

    PubMed

    Kandianis, Catherine B; Michenfelder, Abigail S; Simmons, Susan J; Grusak, Michael A; Stapleton, Ann E

    2013-01-01

    The improvement of grain nutrient profiles for essential minerals and vitamins through breeding strategies is a target important for agricultural regions where nutrient poor crops like maize contribute a large proportion of the daily caloric intake. Kernel iron concentration in maize exhibits a broad range. However, the magnitude of genotype by environment (GxE) effects on this trait reduces the efficacy and predictability of selection programs, particularly when challenged with abiotic stress such as water and nitrogen limitations. Selection has also been limited by an inverse correlation between kernel iron concentration and the yield component of kernel size in target environments. Using 25 maize inbred lines for which extensive genome sequence data is publicly available, we evaluated the response of kernel iron density and kernel mass to water and nitrogen limitation in a managed field stress experiment using a factorial design. To further understand GxE interactions we used partition analysis to characterize response of kernel iron and weight to abiotic stressors among all genotypes, and observed two patterns: one characterized by higher kernel iron concentrations in control over stress conditions, and another with higher kernel iron concentration under drought and combined stress conditions. Breeding efforts for this nutritional trait could exploit these complementary responses through combinations of favorable allelic variation from these already well-characterized genetic stocks.

  15. Abiotic stress growth conditions induce different responses in kernel iron concentration across genotypically distinct maize inbred varieties

    PubMed Central

    Kandianis, Catherine B.; Michenfelder, Abigail S.; Simmons, Susan J.; Grusak, Michael A.; Stapleton, Ann E.

    2013-01-01

    The improvement of grain nutrient profiles for essential minerals and vitamins through breeding strategies is a target important for agricultural regions where nutrient poor crops like maize contribute a large proportion of the daily caloric intake. Kernel iron concentration in maize exhibits a broad range. However, the magnitude of genotype by environment (GxE) effects on this trait reduces the efficacy and predictability of selection programs, particularly when challenged with abiotic stress such as water and nitrogen limitations. Selection has also been limited by an inverse correlation between kernel iron concentration and the yield component of kernel size in target environments. Using 25 maize inbred lines for which extensive genome sequence data is publicly available, we evaluated the response of kernel iron density and kernel mass to water and nitrogen limitation in a managed field stress experiment using a factorial design. To further understand GxE interactions we used partition analysis to characterize response of kernel iron and weight to abiotic stressors among all genotypes, and observed two patterns: one characterized by higher kernel iron concentrations in control over stress conditions, and another with higher kernel iron concentration under drought and combined stress conditions. Breeding efforts for this nutritional trait could exploit these complementary responses through combinations of favorable allelic variation from these already well-characterized genetic stocks. PMID:24363659

  16. Initial Results from the Controlled Shear Decorrelation Experiment (CSDX)

    NASA Astrophysics Data System (ADS)

    Tynan, George; Yun, Seok-Min; Burin, Michael; George, Jonathan

    2000-10-01

    The controlled shear de-correlation experiment (CSDX) has been designed to study the effect of sheared flows on: (1) known linear pressure gradient-driven drift and/or effective gravity-driven flute eigenmodes; (2) the nonlinear three-wave coupling of a finite number of large amplitude coherent modes; and (3) on the rate of electrostatic turbulent fluctuation energy cascades. In CSDX the plasma state (i.e. quiescent, single small-amplitude drift wave, nonlinearly coupled modes, or strongly turbulent) is controlled by varying the magnetic field strength, collisionality, parallel current, and/or effective gravity due to solid-body plasma rotation driven by azimuthal ExB drifts. The radial electric field strength and shear rate is controlled independently of the plasma state by the application of externally controlled voltages to concentric annular rings which will form the ends of the experimental region. Machine construction has been completed and initial results demonstrating all of these capabilities are summarized in this paper. From the fluid equations we show that bispectral analysis of turbulence-scaled processes can yield insight into the key nonlinear physics of shear flow-fluctuation interactions. Initial results and plans using this technique on CSDX data will be discussed.

  17. ALMA test interferometer control system: past experiences and future developments

    NASA Astrophysics Data System (ADS)

    Marson, Ralph G.; Pokorny, Martin; Kern, Jeff; Stauffer, Fritz; Perrigouard, Alain; Gustafsson, Birger; Ramey, Ken

    2004-09-01

    The Atacama Large Millimeter Array (ALMA) will, when it is completed in 2012, be the world's largest millimeter & sub-millimeter radio telescope. It will consist of 64 antennas, each one 12 meters in diameter, connected as an interferometer. The ALMA Test Interferometer Control System (TICS) was developed as a prototype for the ALMA control system. Its initial task was to provide sufficient functionality for the evaluation of the prototype antennas. The main antenna evaluation tasks include surface measurements via holography and pointing accuracy, measured at both optical and millimeter wavelengths. In this paper we will present the design of TICS, which is a distributed computing environment. In the test facility there are four computers: three real-time computers running VxWorks (one on each antenna and a central one) and a master computer running Linux. These computers communicate via Ethernet, and each of the real-time computers is connected to the hardware devices via an extension of the CAN bus. We will also discuss our experience with this system and outline changes we are making in light of our experiences.

  18. Statistical physics of human beings in games: Controlled experiments

    NASA Astrophysics Data System (ADS)

    Liang, Yuan; Huang, Ji-Ping

    2014-07-01

    It is important to know whether the laws or phenomena in statistical physics for natural systems with non-adaptive agents still hold for social human systems with adaptive agents, because this implies whether it is possible to study or understand social human systems by using statistical physics originating from natural systems. For this purpose, we review the role of human adaptability in four kinds of specific human behaviors, namely, normal behavior, herd behavior, contrarian behavior, and hedge behavior. The approach is based on controlled experiments in the framework of market-directed resource-allocation games. The role of the controlled experiments could be at least two-fold: adopting the real human decision-making process so that the system under consideration could reflect the performance of genuine human beings; making it possible to obtain macroscopic physical properties of a human system by tuning a particular factor of the system, thus directly revealing cause and effect. As a result, both computer simulations and theoretical analyses help to show a few counterparts of some laws or phenomena in statistical physics for social human systems: two-phase phenomena or phase transitions, entropy-related phenomena, and a non-equilibrium steady state. This review highlights the role of human adaptability in these counterparts, and makes it possible to study or understand some particular social human systems by means of statistical physics coming from natural systems.

  19. Stress ecology in fucus: abiotic, biotic and genetic interactions.

    PubMed

    Wahl, Martin; Jormalainen, Veijo; Eriksson, Britas Klemens; Coyer, James A; Molis, Markus; Schubert, Hendrik; Dethier, Megan; Karez, Rolf; Kruse, Inken; Lenz, Mark; Pearson, Gareth; Rohde, Sven; Wikström, Sofia A; Olsen, Jeanine L

    2011-01-01

    Stress regimes defined as the synchronous or sequential action of abiotic and biotic stresses determine the performance and distribution of species. The natural patterns of stress to which species are more or less well adapted have recently started to shift and alter under the influence of global change. This was the motivation to review our knowledge on the stress ecology of a benthic key player, the macroalgal genus Fucus. We first provide a comprehensive review of the genus as an ecological model including what is currently known about the major lineages of Fucus species with respect to hybridization, ecotypic differentiation and speciation; as well as life history, population structure and geographic distribution. We then review our current understanding of both extrinsic (abiotic/biotic) and intrinsic (genetic) stress(es) on Fucus species and how they interact with each other. It is concluded that (i) interactive stress effects appear to be equally distributed over additive, antagonistic and synergistic categories at the level of single experiments, but are predominantly additive when averaged over all studies in a meta-analysis of 41 experiments; (ii) juvenile and adult responses to stress frequently differ and (iii) several species or particular populations of Fucus may be relatively unaffected by climate change as a consequence of pre-adapted ecotypes that collectively express wide physiological tolerences. Future research on Fucus should (i) include additional species, (ii) include marginal populations as models for responses to environmental stress; (iii) assess a wider range of stress combinations, including their temporal fluctuations; (iv) better differentiate between stress sensitivity of juvenile versus adult stages; (v) include a functional genomic component in order to better integrate Fucus' ecological and evolutionary responses to stress regimes and (vi) utilize a multivariate modelling approach in order to develop and understand interaction

  20. Overexpression of wheat ubiquitin gene, Ta-Ub2, improves abiotic stress tolerance of Brachypodium distachyon.

    PubMed

    Kang, Hanhan; Zhang, Meng; Zhou, Shumei; Guo, Qifang; Chen, Fengjuan; Wu, Jiajie; Wang, Wei

    2016-07-01

    Ubiquitination plays an important role in regulating plant's development and adaptability to abiotic stress. To investigate the possible functions of a wheat monoubiquitin gene Ta-Ub2 in abiotic stress in monocot and compare it with that in dicot, we generated transgenic Brachypodium plants overexpressing Ta-Ub2 under the control of CaMV35s and stress-inducible RD29A promoters. The constitutive expression of Ta-Ub2 displayed slight growth inhibition in the growth of transgenic Brachypodium distachyon under the control conditions. However, this inhibition was minimized by expression of Ta-Ub2 under the control of stress-inducible RD29A promoter. Compared with WT, the transgenic plants preserved more water and showed higher enzymatic antioxidants under drought stress, which might be related to the change in the expression of some antioxidant genes. The expression of C-repeat binding factors transcription factor genes in the transgenic B. distachyon lines were upregulated under water stress. Salt and cold tolerances of transgenic B. distachyon were also improved. Although the phenotypic changes in the transgenic plants were different, overexpression of Ta-Ub2 improved the abiotic stress tolerance in both dicot and monocot plants. The improvement in Ta-Ub2 transgenic plants in abiotic stress tolerance might be, at least partly, through regulating the gene expression and increasing the enzymatic antioxidants.

  1. Transcriptomic Profiling of the Maize (Zea mays L.) Leaf Response to Abiotic Stresses at the Seedling Stage.

    PubMed

    Li, Pengcheng; Cao, Wei; Fang, Huimin; Xu, Shuhui; Yin, Shuangyi; Zhang, Yingying; Lin, Dezhou; Wang, Jianan; Chen, Yufei; Xu, Chenwu; Yang, Zefeng

    2017-01-01

    Abiotic stresses, including drought, salinity, heat, and cold, negatively affect maize (Zea mays L.) development and productivity. To elucidate the molecular mechanisms of resistance to abiotic stresses in maize, RNA-seq was used for global transcriptome profiling of B73 seedling leaves exposed to drought, salinity, heat, and cold stress. A total of 5,330 differentially expressed genes (DEGs) were detected in differential comparisons between the control and each stressed sample, with 1,661, 2,019, 2,346, and 1,841 DEGs being identified in comparisons of the control with salinity, drought, heat, and cold stress, respectively. Functional annotations of DEGs suggested that the stress response was mediated by pathways involving hormone metabolism and signaling, transcription factors (TFs), very-long-chain fatty acid biosynthesis and lipid signaling, among others. Of the obtained DEGs (5,330), 167 genes are common to these four abiotic stresses, including 10 up-regulated TFs (five ERFs, two NACs, one ARF, one MYB, and one HD-ZIP) and two down-regulated TFs (one b-ZIP and one MYB-related), which suggested that common mechanisms may be initiated in response to different abiotic stresses in maize. This study contributes to a better understanding of the molecular mechanisms of maize leaf responses to abiotic stresses and could be useful for developing maize cultivars resistant to abiotic stresses.

  2. Transcriptomic Profiling of the Maize (Zea mays L.) Leaf Response to Abiotic Stresses at the Seedling Stage

    PubMed Central

    Li, Pengcheng; Cao, Wei; Fang, Huimin; Xu, Shuhui; Yin, Shuangyi; Zhang, Yingying; Lin, Dezhou; Wang, Jianan; Chen, Yufei; Xu, Chenwu; Yang, Zefeng

    2017-01-01

    Abiotic stresses, including drought, salinity, heat, and cold, negatively affect maize (Zea mays L.) development and productivity. To elucidate the molecular mechanisms of resistance to abiotic stresses in maize, RNA-seq was used for global transcriptome profiling of B73 seedling leaves exposed to drought, salinity, heat, and cold stress. A total of 5,330 differentially expressed genes (DEGs) were detected in differential comparisons between the control and each stressed sample, with 1,661, 2,019, 2,346, and 1,841 DEGs being identified in comparisons of the control with salinity, drought, heat, and cold stress, respectively. Functional annotations of DEGs suggested that the stress response was mediated by pathways involving hormone metabolism and signaling, transcription factors (TFs), very-long-chain fatty acid biosynthesis and lipid signaling, among others. Of the obtained DEGs (5,330), 167 genes are common to these four abiotic stresses, including 10 up-regulated TFs (five ERFs, two NACs, one ARF, one MYB, and one HD-ZIP) and two down-regulated TFs (one b-ZIP and one MYB-related), which suggested that common mechanisms may be initiated in response to different abiotic stresses in maize. This study contributes to a better understanding of the molecular mechanisms of maize leaf responses to abiotic stresses and could be useful for developing maize cultivars resistant to abiotic stresses. PMID:28298920

  3. A practical approach for minimum time control of the Spacecraft Control Laboratory Experiment (SCOLE), appendix A

    NASA Technical Reports Server (NTRS)

    Kaufman, H.; Kyriakopoulos, K. J.

    1989-01-01

    The Space COntrol Laboratory Experiment (SCOLE) is a challenge for control engineering applications. This is a result of the system dynamics, the available measurement information, the actuator capabilities and finally the specified performance requirements set. Results on the use of Model Reference Adaptive Control were reported. In view of the necessity for rapid response, this work deals with an optimal control formulation, with a minimum time requirement and constrained input. A mathematical statement of the problem is presented. The time optimal control formulation is presented and the reasons that make such an approach not promising are discussed. As a result, a pseudo time-optimal control algorithm is discussed. The proposed approach is tested to see if it satisfies the design specifications, and finally a discussion and suggestions for further research are provided.

  4. Abiotic dechlorination in rock matrices impacted by long-term exposure to TCE.

    PubMed

    Schaefer, Charles E; Towne, Rachael M; Lippincott, David R; Lacombe, Pierre J; Bishop, Michael E; Dong, Hailiang

    2015-01-01

    Field and laboratory tests were performed to evaluate the abiotic reaction of trichloroethene (TCE) in sedimentary rock matrices. Hydraulically conductive fractures, and the rock directly adjacent to the hydraulically conductive fractures, within a historically contaminated TCE bedrock aquifer were used as the basis for this study. These results were compared to previous work using rock that had not been exposed to TCE (Schaefer et al., 2013) to assess the impact of long-term TCE exposure on the abiotic dechlorination reaction, as the longevity of these reactions after long-term exposure to TCE was hitherto unknown. Results showed that potential abiotic TCE degradation products, including ethane, ethene, and acetylene, were present in the conductive fractures. Using minimally disturbed slices of rock core at and near the fracture faces, laboratory testing on the rocks confirmed that abiotic dechlorination reactions between the rock matrix and TCE were occurring. Abiotic daughter products measured in the laboratory under controlled conditions were consistent with those measured in the conductive fractures, except that propane also was observed as a daughter product. TCE degradation measured in the laboratory was well described by a first order rate constant through the 118-d study. Observed bulk first-order TCE degradation rate constants within the rock matrix were 1.3×10(-8) s(-1). These results clearly show that abiotic dechlorination of TCE is occurring within the rock matrix, despite decades of exposure to TCE. Furthermore, these observed rates of TCE dechlorination are expected to have a substantial impact on TCE migration and uptake/release from rock matrices.

  5. Designing a Hybrid Laminar-Flow Control Experiment: The CFD-Experiment Connection

    NASA Technical Reports Server (NTRS)

    Streett, C. L.

    2003-01-01

    The NASA/Boeing hybrid laminar flow control (HLFC) experiment, designed during 1993-1994 and conducted in the NASA LaRC 8-foot Transonic Pressure Tunnel in 1995, utilized computational fluid dynamics and numerical simulation of complex fluid mechanics to an unprecedented extent for the design of the test article and measurement equipment. CFD was used in: the design of the test wing, which was carried from definition of desired disturbance growth characteristics, through to the final airfoil shape that would produce those growth characteristics; the design of the suction-surface perforation pattern that produced enhanced crossflow-disturbance growth: and in the design of the hot-wire traverse system that produced minimal influence on measured disturbance growth. These and other aspects of the design of the test are discussed, after the historical and technical context of the experiment is described.

  6. Propulsion control experience used in the Highly Integrated Digital Electronic Control (HIDEC) program

    NASA Technical Reports Server (NTRS)

    Myers, L. P.; Burcham, F. W., Jr.

    1984-01-01

    The highly integrated digital electronic control (HIDEC) program will integrate the propulsion and flight control systems on an F-15 airplane at NASA Ames Research Center's Dryden Flight Research Facility. Ames-Dryden has conducted several propulsion control programs that have contributed to the HIDEC program. The digital electronic engine control (DEEC) flight evaluation investigated the performance and operability of the F100 engine equipped with a full-authority digital electronic control system. Investigations of nozzle instability, fault detection and accommodation, and augmentor transient capability provided important information for the HIDEC program. The F100 engine model derivative (EMD) was also flown in the F-15 airplane, and airplane performance was significantly improved. A throttle response problem was found and solved with a software fix to the control logic. For the HIDEC program, the F100 EMD engines equipped with DEEC controls will be integrated with the digital flight control system. The control modes to be implemented are an integrated flightpath management mode and an integrated adaptive engine control system mode. The engine control experience that will be used in the HIDEC program is discussed.

  7. Plasma Rotation Control Experiment in a Strongly Diverging Magnetic Field

    NASA Astrophysics Data System (ADS)

    Terasaka, Kenichiro; Furuta, Kanshi; Yoshimura, Shinji; Aramaki, Mitsutoshi; Tanaka, Masayoshi Y.

    2016-10-01

    It has been recognized that the plasma rotation affects the plasma flow structure along the magnetic field line. However, the effect of plasma rotation on structure formation in a strongly diverging magnetic field with magnetized electrons and unmagnetized ions has not been fully understood, so far. Understanding the flow structure formation in an ion-unmagnetized plasma is essential to control ion streamline detachment from the magnetic field line and also necessary to study the astrophysical phenomena in laboratory. In order to clarify the effect of plasma rotation in a diverging magnetic field, we have performed the plasma rotation control experiment in the HYPER-II device at Kyushu Univ., Japan. A set of cylindrical electrode was utilized to control the radial electric field, and the profile of azimuthal E × B rotation has been changed. We present the experimental results on the electron density pileup and the flow reversal appeared in the rotating plasma. This study was supported by JSPS KAKENHI Grant Number 16K05633.

  8. Dynamics of spacecraft control laboratory experiment (SCOLE) slew maneuvers

    NASA Technical Reports Server (NTRS)

    Kakad, Y. P.

    1987-01-01

    This is the first of two reports on the dynamics and control of slewing maneuvers of the NASA Spacecraft Control Laboratory Experiment (SCOLE). In this report, the dynamics of slewing maneuvers of SCOLE are developed in terms of an arbitrary maneuver about any given axis. The set of dynamical equations incorporate rigid-body slew maneuver and three-dimensional vibrations of the complete assembly comprising the rigid shuttle, the flexible beam, and the reflector with an offset mass. The analysis also includes kinematic nonlinearities of the entire assembly during the maneuver and the dynamics of the interaction between the rigid shuttle and the flexible appendage. The final set of dynamical equations obtained for slewing maneuvers is highly nonlinear and coupled in terms of the flexible modes and the rigid-body modes. The equations are further simplified and evaluated numerically to include the first ten flexible modes and the SCOLE data to yield a model for designing control systems to perform slew maneuvers.

  9. Generation of RNA in abiotic conditions.

    NASA Astrophysics Data System (ADS)

    di Mauro, Ernesto

    Generation of RNA in abiotic conditions. Ernesto Di Mauro Dipartimento di Genetica Bi-ologia Molecolare, Universit` "Sapienza" Roma, Italy. a At least four conditions must be satisfied for the spontaneous generation of (pre)-genetic poly-mers: 1) availability of precursors that are activated enough to spontaneously polymerize. Preliminary studies showed that (a) nucleic bases and acyclonucleosides can be synthesized from formamide H2NCOH by simply heating with prebiotically available mineral catalysts [last reviewed in (1)], and that b) nucleic bases can be phosphorylated in every possible posi-tion [2'; 3'; 5'; cyclic 2',3'; cyclic 3',5' (2)]. The higher stability of the cyclic forms allows their accumulation. 2) A polymerization mechanism. A reaction showing the formation of RNA polymers starting from prebiotically plausible precursors (3',5' cyclic GMP and 3', 5'cyclic AMP) was recently reported (3). Polymerization in these conditions is thermodynamically up-hill and an equilibrium is attained that limits the maximum length of the polymer produced to about 40 nucleotides for polyG and 100 nucleotides for polyA. 3) Ligation of the synthesized oligomers. If this type of reaction could occur according to a terminal-joining mechanism and could generate canonical 3',5' phosphodiester bonds, exponential growth would be obtained of the generated oligomers. This type of reaction has been reported (4) , limited to homogeneous polyA sequences and leading to the production of polyA dimers and tetramers. What is still missing are: 4) mechanisms that provide the proof of principle for the generation of sequence complexity. We will show evidence for two mechanisms providing this proof of principle for simple complementary sequences. Namely: abiotic sequence complementary-driven terminal ligation and sequence-complementary terminal growth. In conclusion: all the steps leading to the generation of RNA in abiotic conditions are satisfied. (1) R Saladino, C Crestini, F

  10. A General Purpose Experiment Controller for low cost Space Application

    NASA Astrophysics Data System (ADS)

    Guzman-Garcia, D.; Rowland, D. E.; Uribe, P.; Nieves-Chinchilla, T.

    2012-12-01

    Space activities are very expensive and include a high degree of risk. Nowadays, CubeSat missions represent a fast and inexpensive way to conduct scientific space research. These platforms are less expensive to develop and build than conventional satellites. There are ample demonstration that these platforms are well suited for a wide range of science missions in different fields, such as astrobiology, astronomy, atmospheric science, space weather and biology. This paper presents a hybrid "processor in an Field Programmable Gate Array (FPGA)" experiment/spacecraft controller for Cubesat missions. The system has two objectives, first is to obtain a multipurpose and easily customizable system aimed at processing the data from the widest kind of instruments and second, to provide the system with the highest processing capabilities in order to be able to perform complex onboard algorithms. Due to the versatility of the system and its reduced dimensions, it can be employed in different space platforms. The system is envisioned to be employed for the first time as the smart radio receiver for the upcoming NASA FireStation instrument. It is one of four experiments manifested to fly on an experiment pallet the U.S Department of Defense plans to deploy on the International Space Station in 2013. FireStation will continue analyzing the link between the Lightning and the Terrestrial Gamma Rays initiated by the FireFly Cubesat. The system is responsible for the management of a set of small Heliophysics instrumentats, including a photometer, magnetometer, and electric and magnetic field antennas. A description of the system architecture and its main features are presented. The main functional and performance tests during the integration and calibration phase of the instruments are also discussed.

  11. Burn Control for the IGNITOR Experiment by External ICRH Heating

    NASA Astrophysics Data System (ADS)

    Cardinali, A.; Coppi, B.; Sonnino, G.

    2012-10-01

    The non-linear thermal balance equation for thermal equilibrium and stability, is analytically and numerically investigated by including the ICRH external wave heating term in order to control the thermonuclear instability in IGNITOR experiment facility. The expressions for ion and electron thermal coefficients, introduced in the thermal balance equation, are obtained by solving the nonlinear transport equations estimated in the several collisional transport regimes (in particular the banana collisional transport regimes). The scaling law of the thermal coefficients with respect to temperature is obtained by fitting the, magnetic surface, averaged profiles of these coefficients against temperature. The ICRH heating in the IGNITOR experiment, among other applications, is expected to stabilize the power of the thermonuclear burning by automatic regulation of the RF coupled power. Here a scenario is considered where IGNITOR is led to operate in a slightly sub-critical regime by adding a small fraction of He3 to the nominal 50-50 Deuterium-Tritium mixture. The difference between power lost and alpha heating is compensated by additional ICRH heating, which should be able to increase the global plasma temperature via collisions between He3 minority and the background D-T ions.

  12. Transgenic expression of TaMYB2A confers enhanced tolerance to multiple abiotic stresses in Arabidopsis.

    PubMed

    Mao, Xinguo; Jia, Dongsheng; Li, Ang; Zhang, Hongying; Tian, Shanjun; Zhang, Xiaoke; Jia, Jizeng; Jing, Ruilian

    2011-09-01

    Osmotic stresses such as drought, salinity, and cold are major environmental factors that limit agricultural productivity. Transcription factors play essential roles in abiotic stress signaling in plants. Three TaMYB2 members were identified and designated TaMYB2A, TaMYB2B, and TaMYB2D based on their genomic origins. The cis-regulatory elements in the promoter regions were compared, and their diverse expression patterns under different abiotic stress conditions were identified. TaMYB2A was further characterized because of its earlier response to stresses. Subcellular localization revealed that TaMYB2A localized in the nucleus. To examine the role of TaMYB2A under various environmental stresses, transgenic Arabidopsis plants carrying TaMYB2A controlled by the CaMV 35S promoter were generated and subjected to severe abiotic stress. TaMYB2A transgenics had enhanced tolerance to drought, salt, and freezing stresses, which were confirmed by the enhanced expressions of abiotic stress-responsive genes and several physiological indices, including decreased rate of water loss, enhanced cell membrane stability, improved photosynthetic potential, and reduced osmotic potential. TaMYB2A is a multifunctional regulatory factor. Its overexpression confers enhanced tolerance to multiple abiotic stresses while having no obvious negative effects on phenotype under well-watered and stressed conditions; thus, TaMYB2A has the potential for utilization in transgenic breeding to improve abiotic stress tolerances in crops.

  13. Abiotic Formation of Methyl Halides in the Terrestrial Environment

    NASA Astrophysics Data System (ADS)

    Keppler, F.

    2011-12-01

    Methyl chloride and methyl bromide are the most abundant chlorine and bromine containing organic compounds in the atmosphere. Since both compounds have relatively long tropospheric lifetimes they can effectively transport halogen atoms from the Earth's surface, where they are released, to the stratosphere and following photolytic oxidation form reactive halogen gases that lead to the chemical destruction of ozone. Methyl chloride and methyl bromide account for more than 20% of the ozone-depleting halogens delivered to the stratosphere and are predicted to grow in importance as the chlorine contribution to the stratosphere from anthropogenic CFCs decline. Today methyl chloride and methyl bromide originate mainly from natural sources with only a minor fraction considered to be of anthropogenic origin. However, until as recently as 2000 most of the methyl chloride and methyl bromide input to the atmosphere was considered to originate from the oceans, but investigations in recent years have clearly demonstrated that terrestrial sources such as biomass burning, wood-rotting fungi, coastal salt marshes, tropical vegetation and organic matter degradation must dominate the atmospheric budgets of these trace gases. However, many uncertainties still exist regarding strengths of both sources and sinks, as well as the mechanisms of formation of these naturally occurring halogenated gases. A better understanding of the atmospheric budget of both methyl chloride and methyl bromide is therefore required for reliable prediction of future ozone depletion. Biotic and abiotic methylation processes of chloride and bromide ion are considered to be the dominant pathways of formation of these methyl halides in nature. In this presentation I will focus on abiotic formation processes in the terrestrial environment and the potential parameters that control their emissions. Recent advances in our understanding of the abiotic formation pathway of methyl halides will be discussed. This will

  14. Abiotic Immobilization of Nitrate in Forest Soils: a Double Label Approach

    NASA Astrophysics Data System (ADS)

    Maclean, R. W.; Ollinger, S. V.; Hobbie, E. A.; Frey, S. D.; Dail, D. B.

    2007-12-01

    Mechanisms of soil nitrogen (N) retention remain a key uncertainty in the terrestrial N cycle. During recent work at the Harvard Forest Chronic N Experiment, 15N added to soils as ammonia nitrate was observed to be rapidly immobilized after addition to soil on a time scale of minutes. In published results it was hypothesized that the rapid time of immobilization could be explained by abiotic immobilization of both ammonia and nitrate. The possibility of abiotic immobilization of nitrate has been studied since the first half of the 20th century, mainly using ideal compounds and soil sterilization techniques. However, critics of these studies have argued that while in vitro studies may indicate the possibility of an abiotic reaction, they cannot demonstrate its plausibility in soils. Soil sterilization methods have been criticized, because they are not effective enough to eliminate biotic interactions within an experimental treatment. Isotopic tracer studies have also been used but also have problems differentiating biotic and abiotic reactions. This study is an attempt to demonstrate abiotic immobilization of nitrate in soil samples through the use of double labeled nitrate (15N18O3- ). The resolution of this method depends on the biochemistry of microbial immobilization of nitrate; reduction of nitrate to nitrite, then ammonia and glutamine before incorporation into microbial biomass. Reduction of 15N18O3- before microbial utilization of the 15N implies that retention of both heavy isotopes in the soil can only occur through abiotic reaction of 15N18Ox species. In biotic immobilization the 18O is lost to the system in water. While nitrate has proven unreactive in soils, its reduced product, nitrite, is known to be readily reactive with various soil compounds. Nitrite can be introduced into the soil environment naturally by both 'leakiness' in nitrification and denitrification and may possibly be generated abiotically through methods such as the proposed Ferrous

  15. Invasive earthworms interact with abiotic conditions to influence the invasion of common buckthorn (Rhamnus cathartica).

    PubMed

    Roth, Alexander M; Whitfeld, Timothy J S; Lodge, Alexandra G; Eisenhauer, Nico; Frelich, Lee E; Reich, Peter B

    2015-05-01

    Common buckthorn (Rhamnus cathartica L.) is one of the most abundant and ecologically harmful non-native plants in forests of the Upper Midwest United States. At the same time, European earthworms are invading previously glaciated areas in this region, with largely anecdotal evidence suggesting they compound the negative effects of buckthorn and influence the invasibility of these forests. Germination and seedling establishment are important control points for colonization by any species, and manipulation of the conditions influencing these life history stages may provide insight into why invasive species are successful in some environments and not others. Using a greenhouse microcosm experiment, we examined the effects of important biotic and abiotic factors on the germination and seedling establishment of common buckthorn. We manipulated light levels, leaf litter depth and earthworm presence to investigate the independent and interactive effects of these treatments on buckthorn establishment. We found that light and leaf litter depth were significant predictors of buckthorn germination but that the presence of earthworms was the most important factor; earthworms interacted with light and leaf litter to increase the number and biomass of buckthorn across all treatments. Path analysis suggested both direct and moisture-mediated indirect mechanisms controlled these processes. The results suggest that the action of earthworms may provide a pathway through which buckthorn invades forests of the Upper Midwest United States. Hence, researchers and managers should consider co-invasion of plants and earthworms when investigating invasibility and creating preemptive or post-invasion management plans.

  16. Cell wall remodeling under abiotic stress

    PubMed Central

    Tenhaken, Raimund

    2015-01-01

    Plants exposed to abiotic stress respond to unfavorable conditions on multiple levels. One challenge under drought stress is to reduce shoot growth while maintaining root growth, a process requiring differential cell wall synthesis and remodeling. Key players in this process are the formation of reactive oxygen species (ROS) and peroxidases, which initially cross-link phenolic compounds and glycoproteins of the cell walls causing stiffening. The function of ROS shifts after having converted all the peroxidase substrates in the cell wall. If ROS-levels remain high during prolonged stress, OH°-radicals are formed which lead to polymer cleavage. In concert with xyloglucan modifying enzymes and expansins, the resulting cell wall loosening allows further growth of stressed organs. PMID:25709610

  17. Abiotic formation of oligonucleotides on basalt surfaces

    NASA Astrophysics Data System (ADS)

    Otroshchenko, V. A.; Vasilyeva, N. V.; Kopilov, A. M.

    1985-06-01

    The complication and further evolution of abiotic syntheses products occurred under environmental influences at the prebiological stage. From this point of view, the influence of some types of irradiation on the organic molecules adsorbed on the surfaces of volcanic rocks, appeared to be of great importance. In this connection, the effect of gamma rays on the AMP molecules adsorbed on mineral surfaces such as cinders and ashes has been studied. It has been shown that they can polymerize with the formation of oligonucleotides. The treatment of oligomers obtained by venom phosphodiesterase has shown that a polymeric product has mainly 3' 5' and 2' 5' bonds between nucleotides. The results obtained have been discussed from the evolutionary aspect.

  18. AMO EXPRESS: A Command and Control Experiment for Crew Autonomy

    NASA Technical Reports Server (NTRS)

    Stetson, Howard K.; Frank, Jeremy; Cornelius, Randy; Haddock, Angie; Wang, Lui; Garner, Larry

    2015-01-01

    NASA is investigating a range of future human spaceflight missions, including both Mars-distance and Near Earth Object (NEO) targets. Of significant importance for these missions is the balance between crew autonomy and vehicle automation. As distance from Earth results in increasing communication delays, future crews need both the capability and authority to independently make decisions. However, small crews cannot take on all functions performed by ground today, and so vehicles must be more automated to reduce the crew workload for such missions. NASA's Advanced Exploration Systems Program funded Autonomous Mission Operations (AMO) project conducted an autonomous command and control demonstration of intelligent procedures to automatically initialize a rack onboard the International Space Station (ISS) with power and thermal interfaces, and involving core and payload command and telemetry processing, without support from ground controllers. This autonomous operations capability is enabling in scenarios such as a crew medical emergency, and representative of other spacecraft autonomy challenges. The experiment was conducted using the Expedite the Processing of Experiments for Space Station (EXPRESS) rack 7, which was located in the Port 2 location within the U.S Laboratory onboard the International Space Station (ISS). Activation and deactivation of this facility is time consuming and operationally intensive, requiring coordination of three flight control positions, 47 nominal steps, 57 commands, 276 telemetry checks, and coordination of multiple ISS systems (both core and payload). The autonomous operations concept includes a reduction of the amount of data a crew operator is required to verify during activation or de-activation, as well as integration of procedure execution status and relevant data in a single integrated display. During execution, the auto-procedures provide a step-by-step messaging paradigm and a high level status upon termination. This

  19. ABIOTIC IN SITU TECHNOLOGIES FOR GROUNDWATER REMEDIATION CONFERENCE: PROCEEDINGS

    EPA Science Inventory

    The USEPA conference on Abiotic In Situ Technologies for Groundwater Remediation was held in Dallas, TX, 8/31-9/2/99. The goal of the meeting was to disseminate current information on abiotic in situ groundwater treatment echnologies. Although much information is being provided a...

  20. The 5th Annual NASA Spacecraft Control Laboratory Experiment (SCOLE) Workshop, part 2

    NASA Technical Reports Server (NTRS)

    Taylor, Lawrence W., Jr. (Compiler)

    1990-01-01

    A collection of papers from the workshop are presented. The topics addressed include: the modeling, systems identification, and control synthesis for the Spacecraft Control Laboratory Experiment (SCOLE) configuration.

  1. Mathematical inference and control of molecular networks from perturbation experiments

    NASA Astrophysics Data System (ADS)

    Mohammed-Rasheed, Mohammed

    in order to affect the time evolution of molecular activity in a desirable manner. In this proposal, we address both the inference and control problems of GRNs. In the first part of the thesis, we consider the control problem. We assume that we are given a general topology network structure, whose dynamics follow a discrete-time Markov chain model. We subsequently develop a comprehensive framework for optimal perturbation control of the network. The aim of the perturbation is to drive the network away from undesirable steady-states and to force it to converge to a unique desirable steady-state. The proposed framework does not make any assumptions about the topology of the initial network (e.g., ergodicity, weak and strong connectivity), and is thus applicable to general topology networks. We define the optimal perturbation as the minimum-energy perturbation measured in terms of the Frobenius norm between the initial and perturbed networks. We subsequently demonstrate that there exists at most one optimal perturbation that forces the network into the desirable steady-state. In the event where the optimal perturbation does not exist, we construct a family of sub-optimal perturbations that approximate the optimal solution arbitrarily closely. In the second part of the thesis, we address the inference problem of GRNs from time series data. We model the dynamics of the molecules using a system of ordinary differential equations corrupted by additive white noise. For large-scale networks, we formulate the inference problem as a constrained maximum likelihood estimation problem. We derive the molecular interactions that maximize the likelihood function while constraining the network to be sparse. We further propose a procedure to recover weak interactions based on the Bayesian information criterion. For small-size networks, we investigated the inference of a globally stable 7-gene melanoma genetic regulatory network from genetic perturbation experiments. We considered five

  2. Improved abiotic stress tolerance of bermudagrass by exogenous small molecules.

    PubMed

    Chan, Zhulong; Shi, Haitao

    2015-01-01

    As a widely used warm-season turfgrass in landscapes and golf courses, bermudagrass encounters multiple abiotic stresses during the growth and development. Physiology analysis indicated that abiotic stresses induced the accumulation of ROS and decline of photosynthesis, resulting in increased cell damage and inhibited growth. Proteomic and metabolomic approaches showed that antioxidant enzymes and osmoprotectant contents (sugar, sucrose, dehydrin, proline) were extensively changed under abiotic stress conditions. Exogenous application of small molecules, such as ABA, NO, CaCl2, H2S, polyamine and melatonin, could effectively alleviate damages caused by multiple abiotic stresses, including drought, salt, heat and cold. Based on high through-put RNA seq analysis, genes involved in ROS, transcription factors, hormones, and carbohydrate metabolisms were largely enriched. The data indicated that small molecules induced the accumulation of osmoprotectants and antioxidants, kept cell membrane integrity, increased photosynthesis and kept ion homeostasis, which protected bermudagrass from damages caused by abiotic stresses.

  3. Abiotic stresses induce different localizations of anthocyanins in Arabidopsis

    PubMed Central

    Kovinich, Nik; Kayanja, Gilbert; Chanoca, Alexandra; Otegui, Marisa S; Grotewold, Erich

    2015-01-01

    Anthocyanins are induced in plants in response to abiotic stresses such as drought, high salinity, excess light, and cold, where they often correlate with enhanced stress tolerance. Numerous roles have been proposed for anthocyanins induced during abiotic stresses including functioning as ROS scavengers, photoprotectants, and stress signals. We have recently found different profiles of anthocyanins in Arabidopsis (Arabidopsis thaliana) plants exposed to different abiotic stresses, suggesting that not all anthocyanins have the same function. Here, we discuss these findings in the context of other studies and show that anthocyanins induced in Arabidopsis in response to various abiotic stresses have different localizations at the organ and tissue levels. These studies provide a basis to clarify the role of particular anthocyanin species during abiotic stress. PMID:26179363

  4. Biotic and abiotic degradation behaviour of ethylene glycol monomethyl ether (EGME).

    PubMed

    Fischer, A; Hahn, C

    2005-05-01

    Glycol ethers are widely used in many processes in the chemical industry. Their high water solubility means they are used as solvents for different purposes (e.g. lacquers and varnishes). Since glycol ethers are known to produce toxic metabolites such as the teratogenic methoxyacetic acid during biodegradation, the biological treatment of glycol ethers can be hazardous. However, using oxidizing agents like hydrogen peroxide could be a feasible option for treating wastewater containing glycol ether. In this study, both-, biodegradation and abiotic oxidation experiments with ethylene glycol monomethyl ether (EGME) as contaminant were performed. The biodegradation experiments were conducted with a synthetic model wastewater containing 15 wt% NaCl and 5000 mgl(-1) of EGME. While experiments with the fungus Aspergillus versicolor resulted in the exhaustive biotic degradation of EGME, the toxic metabolite methoxyacetic acid (MAA) was produced as a 'dead end' product. Sodium hydroxide was added to adjust the decreasing pH caused by the production of MAA. In abiotic degradation experiments with EGME, other degradation products--organic acids and toxic aldehydes, e.g. methoxy acetaldehyde (MALD)--were detected. It must be taken into account that EGME and its biotic and abiotic degradation products are usually not analysed in routine wastewater measurements owing to their physical properties.

  5. miRNAs: Major modulators for crop growth and development under abiotic stresses.

    PubMed

    Noman, Ali; Fahad, Shah; Aqeel, Muhammad; Ali, Usman; Amanullah; Anwar, Sumera; Baloch, Shahbaz Khan; Zainab, Madiha

    2017-02-25

    Cumulatively, biotic and abiotic stresses of various magnitudes can decrease the production of crops by 70%. miRNAs have emerged as a genetic tool with enormous potential that can be exploited to understand stress tolerance at the molecular level and eventually regulate stress in crops. Plant miRNA targets frequently fit into diverse families of TFs that control the expression of genes related to a certain trait. As key machinery in gene regulatory networks, it is agreed that a broad understanding of miRNAs will greatly increase our understanding of plant responses to environmental stresses. miRNA-led stress regulatory networks are being considered as novel tools for the development of abiotic stress tolerance in crops. At this time, we need to expand our knowledge about the modulatory role of miRNAs during environmental fluctuations. It has become exceedingly clear that with increased understanding of the role of miRNAs during stress, the techniques for using miRNA-mediated gene regulation to enhance plant stress tolerance will become more effective and reliable. In this review we present: (1) miRNAs as a potential avenue for the modulation of abiotic stresses, and (2) summarize the research progress regarding plant responses to stress. Current progress is explained through discussion of the identification and validation of several miRNAs that enhance crop tolerance of salinity, drought, etc., while missing links on different aspects of miRNAs related to abiotic stress tolerance are noted.

  6. Jasmonate signaling in plant development and defense response to multiple (a)biotic stresses.

    PubMed

    Santino, Angelo; Taurino, Marco; De Domenico, Stefania; Bonsegna, Stefania; Poltronieri, Palmiro; Pastor, Victoria; Flors, Victor

    2013-07-01

    Plants frequently live in environments characterized by the presence of simultaneous and different stresses. The intricate and finely tuned molecular mechanisms activated by plants in response to abiotic and biotic environmental factors are not well understood, and less is known about the integrative signals and convergence points activated by plants in response to multiple (a)biotic stresses. Phytohormones play a key role in plant development and response to (a)biotic stresses. Among these, one of the most important signaling molecules is an oxylipin, the plant hormone jasmonic acid. Oxylipins are derived from oxygenation of polyunsaturated fatty acids. Jasmonic acid and its volatile derivative methyl jasmonate have been considered for a long time to be the bioactive forms due to their physiological effects and abundance in the plant. However, more recent studies showed unambiguously that they are only precursors of the active forms represented by some amino acid conjugates. Upon developmental or environmental stimuli, jasmonates are synthesized and accumulate transiently. Upon perception, jasmonate signal transduction process is finely tuned by a complex mechanism comprising specific repressor proteins which in turn control a number of transcription factors regulating the expression of jasmonate responsive genes. We discuss the latest discoveries about the role of jasmonates in plants resistance mechanism against biotic and abiotic stresses. Finally, the deep interplay of different phytohormones in stresses signaling will be also discussed.

  7. Space Technology Experiment Platform (STEP). A Shuttle-borne support facility for structures, structural dynamics, and control technology flight experiments

    NASA Technical Reports Server (NTRS)

    Harris, J. E.; Pinson, L. D.

    1983-01-01

    The Space Transportation System (STS) is used for technology experiments in space. The Space Technology Experiment Platform (STEP) is a Shuttle-borne experiment support facility for use by structures, structural dynamics, and controls technology flight experiments. STEP represents a key element in the commitment to STS utilization. The STEP concept and definition process is discussed, and the results obtained to date on the configuration and function capability are summarized, and preliminary schedule information is presented.

  8. Abiotic factors and trap design modulate the performance of traps used to monitor the plum curculio.

    PubMed

    Lamothe, Steve; Chouinard, Gérald; Vincent, Charles

    2008-12-01

    All published studies on effects of abiotic factors on plum curculio, Conotrachelus nenuphar (Hersbt), adults have taken a retrospective approach. Here, we present the results of experiments where factors and their levels were determined and controlled a priori. We compared the effectiveness of miniature pyramidal traps (45 by 20 by 20 cm) constructed of four kind of materials--wood, geotextile, nylon screening, and corrugated plastic--to monitor overwintered and summer adults of univoltine plum curculio. We also studied the effects of photoperiod, temperature, wind, and rain on pyramidal trap effectiveness. The experiments, which were replicated over time, were done in two controlled chambers that were divided into four sections, corresponding to simulated combinations (wind or no wind/rain or no rain). The temperatures tested (15, 20, and 25 degrees C) were randomly assigned in the chambers. During scotophase, geotextile traps captured significantly more overwintered and summer adults than traps made of other materials. The maximum proportion of captures (for overwintered and summer adults) during photophase was obtained at 25 degrees C, and it was significantly different than captures at 15 and 20 degrees C. During scotophase, significantly more overwintered and summer plum curculios were caught at 20 and 25 degrees C than at 15 degrees C. Our experiments demonstrated that geotextile is a good alternative to wooden pyramidal trap. Our results suggest that captures were higher 1) at night, 2) during warmer periods (20 and 25 degrees C), 3) when wind velocity was low and 4) during or shortly after rainfall, and 5) that photoperiod is a factor having an important predictive value for plum curculio captures.

  9. Wood smoke in a controlled exposure experiment with human volunteers.

    PubMed

    Riddervold, I S; Bønløkke, J H; Mølhave, L; Massling, A; Jensen, B; Grønborg, T K; Bossi, R; Forchhammer, L; Kjærgaard, S K; Sigsgaard, T

    2011-04-01

    Exposure to wood smoke in the general population is increasing and concurrently, also our awareness. This article describes a wood-smoke generating system for studying human exposure to wood smoke and symptoms related to this exposure. Twenty nonsmoking atopic human participants with normal lung function and normal bronchial reactivity were randomly exposed for 3 h at three different exposure conditions; clean filtered air (control exposure) and wood smoke with a characteristic particulate matter (PM) concentration of 200 µg/m³ (low) and 400 µg/m³ (high) under controlled environmental conditions. The range for PM₂.₅ load observed for single experiments was 165-303 µg/m³ for the low exposure and 205-662 µg/m³ for the high exposure, whereas particle loads during clean air exposure most often were below the detection limit (< 20 µg/m³). Health effects were evaluated in relation to rated changes in symptoms and environmental perception using a computerized questionnaire and a potentiometer. Subjective symptoms were generally weak, but when combining the effect of each of the symptoms into categorical symptom indices, significant effects were found for "environmental perception" (p = 0.0007), "irritative body perceptions" (p = 0.0127), "psychological/neurological effects" (p = 0.0075) and "weak inflammatory responses" (p = 0.0003). Furthermore, significant effects (p = 0.0192) on self-reported general mucosa irritation were found. In conclusion, exposure to wood smoke affected symptom rating and caused irritated mucosas in humans. The knowledge gained in this study on subjective-rated symptoms may be important for understanding human response to wood-smoke exposure.

  10. Understanding controlled drug release from mesoporous silicates: theory and experiment.

    PubMed

    Ukmar, T; Maver, U; Planinšek, O; Kaučič, V; Gaberšček, M; Godec, A

    2011-11-07

    Based on the results of carefully designed experiments upgraded with appropriate theoretical modeling, we present clear evidence that the release curves from mesoporous materials are significantly affected by drug-matrix interactions. In experimental curves, these interactions are manifested as a non-convergence at long times and an inverse dependence of release kinetics on pore size. Neither of these phenomena is expected in non-interacting systems. Although both phenomena have, rather sporadically, been observed in previous research, they have not been explained in terms of a general and consistent theoretical model. The concept is demonstrated on a model drug indomethacin embedded into SBA-15 and MCM-41 porous silicates. The experimental release curves agree exceptionally well with theoretical predictions in the case of significant drug-wall attractions. The latter are described using a 2D Fokker-Planck equation. One could say that the interactions affect the relative cross-section of pores where the local flux has a non-vanishing axial component and in turn control the effective transfer of drug into bulk solution. Finally, we identify the critical parameters determining the pore size dependence of release kinetics and construct a dynamic phase diagram of the various resulting transport regimes.

  11. Oxygen fugacity control in piston-cylinder experiments

    NASA Astrophysics Data System (ADS)

    Jakobsson, Sigurdur

    2012-09-01

    The main goal of this study was to develop and test a capsule assembly for use in piston-cylinder experiments where oxygen fugacity could be controlled in the vicinity of the QFM buffer without H2O loss or carbon contamination of the sample material. The assembly consists of an outer Pt-capsule containing a solid buffer (Ni-NiO or Co-CoO) plus H2O and an inner AuPd-capsule, containing the sample, H2O and a Pt-wire. No H2O loss is observed from the sample, even after 48 h, but a slight increase in H2O content is found in longer runs due to oxygen and hydrogen diffusion into the AuPd-capsule. Oxygen fugacity of runs in equilibrium with the Ni-NiO (NNO) and Co-CoO (CoCO) buffers was measured by analyzing Fe dissolved in the Pt-wire and in the AuPd-capsule. The second method gives values that are in good agreement with established buffer values, whereas results from the first method are one half to one log units higher than the established values.

  12. Current profile control experiments in EXTRAP T2R

    NASA Astrophysics Data System (ADS)

    Brunsell, P.; Cecconello, M.; Drake, J.; Franz, P.; Malmberg, J. A.; Marrelli, L.; Martin, P.; Spizzo, G.

    2002-11-01

    EXTRAP T2R is a high aspect ratio (R=1.24 m, a = 0.183 m) reversed-field pinch device, characterised by a double, thin shell system. The simultaneous presence of many m=1, |n| > 11 tearing modes is responsible for a magnetic field turbulence, which is believed to produce the rather high energy and particle transport that is observed in this type of magnetic configuration. In this paper first results from current profile control experiments (PPCD) in a thin shell device are shown. When an edge poloidal electric field is transiently applied, an increase of the electron temperature and of the electron density is seen, which is consistent with an increase of the thermal content of the plasma. At the same time, the soft x-ray emission, measured with a newly installed miniaturised camera, shows a peaking of the profile in the core. Furthermore, the amplitudes of the m=1 tearing modes are reduced and and the rotation velocities increase during PPCD, which is also consistent with a reduction of magnetic turbulence and a heating of the plasma

  13. The Space Technology-7 Disturbance Reduction System Precision Control Flight Validation Experiment Control System Design

    NASA Technical Reports Server (NTRS)

    O'Donnell, James R.; Hsu, Oscar C.; Maghami, Peirman G.; Markley, F. Landis

    2006-01-01

    As originally proposed, the Space Technology-7 Disturbance Reduction System (DRS) project, managed out of the Jet Propulsion Laboratory, was designed to validate technologies required for future missions such as the Laser Interferometer Space Antenna (LISA). The two technologies to be demonstrated by DRS were Gravitational Reference Sensors (GRSs) and Colloidal MicroNewton Thrusters (CMNTs). Control algorithms being designed by the Dynamic Control System (DCS) team at the Goddard Space Flight Center would control the spacecraft so that it flew about a freely-floating GRS test mass, keeping it centered within its housing. For programmatic reasons, the GRSs were descoped from DRS. The primary goals of the new mission are to validate the performance of the CMNTs and to demonstrate precise spacecraft position control. DRS will fly as a part of the European Space Agency (ESA) LISA Pathfinder (LPF) spacecraft along with a similar ESA experiment, the LISA Technology Package (LTP). With no GRS, the DCS attitude and drag-free control systems make use of the sensor being developed by ESA as a part of the LTP. The control system is designed to maintain the spacecraft s position with respect to the test mass, to within 10 nm/the square root of Hz over the DRS science frequency band of 1 to 30 mHz.

  14. Volcanic Monitoring Techniques Applied to Controlled Fragmentation Experiments

    NASA Astrophysics Data System (ADS)

    Kueppers, U.; Alatorre-Ibarguengoitia, M. A.; Hort, M. K.; Kremers, S.; Meier, K.; Scharff, L.; Scheu, B.; Taddeucci, J.; Dingwell, D. B.

    2010-12-01

    Volcanic eruptions are an inevitable natural threat. The range of eruptive styles is large and short term fluctuations of explosivity or vent position pose a large risk that is not necessarily confined to the immediate vicinity of a volcano. Explosive eruptions rather may also affect aviation, infrastructure and climate, regionally as well as globally. Multiparameter monitoring networks are deployed on many active volcanoes to record signs of magmatic processes and help elucidate the secrets of volcanic phenomena. However, our mechanistic understanding of many processes hiding in recorded signals is still poor. As a direct consequence, a solid interpretation of the state of a volcano is still a challenge. In an attempt to bridge this gap, we combined volcanic monitoring and experimental volcanology. We performed 15 well-monitored, field-based, experiments and fragmented natural rock samples from Colima volcano (Mexico) by rapid decompression. We used cylindrical samples of 60 mm height and 25 mm and 60 mm diameter, respectively, and 25 and 35 vol.% open porosity. The applied pressure range was from 4 to 18 MPa. Using different experimental set-ups, the pressurised volume above the samples ranged from 60 - 170 cm3. The experiments were performed at ambient conditions and at controlled sample porosity and size, confinement geometry, and applied pressure. The experiments have been thoroughly monitored with 1) Doppler Radar (DR), 2) high-speed and high-definition cameras, 3) acoustic and infrasound sensors, 4) pressure transducers, and 5) electrically conducting wires. Our aim was to check for common results achieved by the different approaches and, if so, calibrate state-of-the-art monitoring tools. We present how the velocity of the ejected pyroclasts was measured by and evaluated for the different approaches and how it was affected by the experimental conditions and sample characteristics. We show that all deployed instruments successfully measured the pyroclast

  15. Pressure Effects on the Abiotic Polymerization of Glycine

    NASA Astrophysics Data System (ADS)

    Ohara, Shohei; Kakegawa, Takeshi; Nakazawa, Hiromoto

    2007-06-01

    Polymerization experiments were performed using dry glycine under various pressures of 5 100 MPa at 150°C for 1 32 days. The series of experiments was carried out under the assumption that the pore space of deep sediments was adequate for dehydration polymerization of pre-biotic molecules. The products show various colors ranging from dark brown to light yellow, depending on the pressure. Visible and infrared spectroscopy reveal that the coloring is the result of formation of melanoidins at lower pressures. High-performance liquid chromatography and mass spectrometry analyses of the products show that: (1) glycine in all the experimental runs oligomerizes from 2-mer to 10-mer; (2) the yields are dependent on pressure up to 25 MPa and decrease slightly thereafter; and (3) polymerization progressed for the first 8 days, while the amounts of oligomers remained constant for longer-duration runs of up to 32 days. These results suggest that pressure inhibits the decomposition of amino acids and encourages polymerization in the absence of a catalyst. Our results further imply that abiotic polymerization could have occurred during diagenesis in deep sediments rather than in oceans.

  16. Photo Bleaching of Dissolved Organic Matter Enhances Abiotic Greenhouse Gas Emissions but Inhibits Biotic Emissions

    NASA Astrophysics Data System (ADS)

    Wang, J.; Chow, A. T.; Ng, T.; Wong, P.

    2013-12-01

    Greenhouse gas (GHG) emission from aquatic sources is one of the essential processes in the global carbon cycling. The natural Fenton reaction is commonly occurring in sunlited environment, affecting the degradation of dissolved organic matters (DOMs) and many other biogeochemical processes. In order to evaluate the effect of natural Fenton reaction on the CH4 and CO2 emissions from DOMs, different sources (wetland surface water, wetland soil pore water, and plant litter leachates) of organic matters were incubated under controlled laboratory condition with different dosages of Fenton reagents and environmental conditions. The GHG emissions depended on the dose of Fenton-reagents, reaction time, temperature, and light intensity. Abiotically, the DOMs were photo-degraded into GHGs by both the direct and indirect photolysis. Yet biotically, the reactive oxidative species (ROSs) generated from sunlited waters inactivated the microbes and thus inhibited the biotic GHG emissions. Results of our experiments demonstrate that the dual roles of photo-bleaching of DOM on GHG emission from sunlited surface waters.

  17. Real-Time Manganese Phase Dynamics during Biological and Abiotic Manganese Oxide Reduction.

    PubMed

    Johnson, Jena E; Savalia, Pratixa; Davis, Ryan; Kocar, Benjamin D; Webb, Samuel M; Nealson, Kenneth H; Fischer, Woodward W

    2016-04-19

    Manganese oxides are often highly reactive and easily reduced, both abiotically, by a variety of inorganic chemical species, and biologically during anaerobic respiration by microbes. To evaluate the reaction mechanisms of these different reduction routes and their potential lasting products, we measured the sequence progression of microbial manganese(IV) oxide reduction mediated by chemical species (sulfide and ferrous iron) and the common metal-reducing microbe Shewanella oneidensis MR-1 under several endmember conditions, using synchrotron X-ray spectroscopic measurements complemented by X-ray diffraction and Raman spectroscopy on precipitates collected throughout the reaction. Crystalline or potentially long-lived phases produced in these experiments included manganese(II)-phosphate, manganese(II)-carbonate, and manganese(III)-oxyhydroxides. Major controls on the formation of these discrete phases were alkalinity production and solution conditions such as inorganic carbon and phosphate availability. The formation of a long-lived Mn(III) oxide appears to depend on aqueous Mn(2+) production and the relative proportion of electron donors and electron acceptors in the system. These real-time measurements identify mineralogical products during Mn(IV) oxide reduction, contribute to understanding the mechanism of various Mn(IV) oxide reduction pathways, and assist in interpreting the processes occurring actively in manganese-rich environments and recorded in the geologic record of manganese-rich strata.

  18. The dynamics and control of the orbiting spacecraft control laboratory experiment (SCOLE) during station keeping

    NASA Technical Reports Server (NTRS)

    Bainum, Peter M.; Diarra, Cheick M.

    1988-01-01

    A mathematical model is developed to predict the dynamics of the proposed orbiting Spacecraft Control Laboratory Experiment during the station keeping phase. The Shuttle as well as the reflector are assumed to be rigid, the mast is flexible and is assumed to undergo elastic displacements very small as compared with its length. The equations of motion are derived using a Newton-Euler formulation. The model includes the effects of gravity, flexibility, and orbital dynamics. The control is assumed to be provided to the system through the Shuttle's three torquers, and through six actuators located by pairs at two points on the mast and at the mass center of the reflector. At each of the locations, an actuator acts parallel to the roll axis while the other one acts parallel to the pitch axis. It is seen that, in the presence of gravity-gradient torques in the system dynamics, the system assumes a new equilibrium position about which the equations must be linearized, primarily due to the offset in the mast attachment point to the reflector. The linear regulator theory is used to derive control laws for the linear model of the SCOLE including the first four flexible modes. Numerical results confirm the robustness of this control strategy for station keeping with maximum control efforts significantly below saturation levels.

  19. Recent Molecular Advances on Downstream Plant Responses to Abiotic Stress

    PubMed Central

    dos Reis, Sávio Pinho; Lima, Aline Medeiros; de Souza, Cláudia Regina Batista

    2012-01-01

    Abiotic stresses such as extremes of temperature and pH, high salinity and drought, comprise some of the major factors causing extensive losses to crop production worldwide. Understanding how plants respond and adapt at cellular and molecular levels to continuous environmental changes is a pre-requisite for the generation of resistant or tolerant plants to abiotic stresses. In this review we aimed to present the recent advances on mechanisms of downstream plant responses to abiotic stresses and the use of stress-related genes in the development of genetically engineered crops. PMID:22942725

  20. The White Pine Mine explosively induced, controlled collapse experiment

    SciTech Connect

    Pearson, D.C.; Stump, B.W.; Phillips, W.S.

    1996-09-01

    On September 3, 1995, the White Pine Mine, which is owned by Copper Range Company, conducted the first of a planned series of explosive removal of existing pillars in their underground mining operations. The purpose of this operation is to evaluate the effectiveness of pillar rubbilization and roof collapse for planned in-situ leaching of the copper ore from the rock mass. This type of seismic source is unique in that a large, delay fired, explosive source was expected to be followed by collapse of the rock immediately above the explosion into the void created. Characterization of this type of mining source is of interest to the Comprehensive Test Ban Treaty (CTBT) R&D Seismic Program due to its unique properties. These include the controlled nature of the source in time, location, and magnitude, the fact that the source is located in an active region of underground mining, and that natural collapse of large portions of this mine have occurred in the recent past. The Mine operator is concerned with the characterization of the vibration induced by both the explosive and implosive components of the procedure and determination of the depth to which chimneying of the roof proceeded. This report will document: The reasons for conducting both the explosively induced collapse and the Los Alamos National Laboratory CTBT R&D Experimental Field Program experiment; The local and regional seismic, acoustic, and videographic data acquired; Analysis of the explosion/collapse seismic signal generated; Analysis and location of the aftershocks associated with the collapse; and Conclusions made concerning this type of mining explosion in relation to verification of a Comprehensive Test Ban Treaty.

  1. Can nutrient pathways and biotic interactions control eutrophication in riverine ecosystems? Evidence from a model driven mesocosm experiment.

    PubMed

    Jäger, Christoph G; Hagemann, Jeske; Borchardt, Dietrich

    2017-05-15

    Ecological theory predicts that the relative importance of benthic to planktonic primary production usually changes along the rivers' continuum from a predomination of benthic algae in lower stream orders to a predomination of planktonic algae at higher orders. Underlying mechanisms driving the interaction between algae in these habitats, its controlling factors and consequences for riverine ecosystems are, however, only partly understood. We present a mechanistic analysis of the governing ecological processes using a simplified, numerical model and examine how abiotic factors and biotic interactions influence benthic and planktonic algae by changing resource competition. We compare the outcome of the model with the results of a factorial mesocosm experiment mimicking the parameter spaces of the model. The results show a remarkable similarity with regard to the temporal development of benthic and pelagic algal biomass and shifting dominance patterns. In particular we analyse the effects of the pathways of nutrient supply (upwelling from the hyporheic zone, direct supply to the surface water, or via both pathways) and grazing in a gradient of river depths. Our results show that detachment of benthic algae, sinking of planktonic algae and the pathway of nutrient supply are key processes determining the respective algal biomass distributions particularly in shallow and intermediate deep systems. Increasing nutrient supply increases algal biomasses, but does not change the general pattern of the interactions. Decreasing light supply decreases the dominance of planktonic algae, but increases dissolved nutrients. At intermediate to high grazing rates algal biomass can be controlled by grazers, but however, at high grazing rates, dissolved nutrients accumulate in the surface water. Our results indicate that nutrient pathways, resource competition and internal control by grazing need to be considered explicitly for the understanding and explanation of eutrophication

  2. Polyamines and abiotic stress tolerance in plants

    PubMed Central

    Gill, Sarvajeet Singh

    2010-01-01

    Environmental stresses including climate change, especially global warming, are severely affecting plant growth and productivity worldwide. It has been estimated that two-thirds of the yield potential of major crops are routinely lost due to the unfavorable environmental factors. On the other hand, the world population is estimated to reach about 10 billion by 2050, which will witness serious food shortages. Therefore, crops with enhanced vigour and high tolerance to various environmental factors should be developed to feed the increasing world population. Maintaining crop yields under adverse environmental stresses is probably the major challenge facing modern agriculture where polyamines can play important role. Polyamines (PAs)(putrescine, spermidine and spermine) are group of phytohormone-like aliphatic amine natural compounds with aliphatic nitrogen structure and present in almost all living organisms including plants. Evidences showed that polyamines are involved in many physiological processes, such as cell growth and development and respond to stress tolerance to various environmental factors. In many cases the relationship of plant stress tolerance was noted with the production of conjugated and bound polyamines as well as stimulation of polyamine oxidation. Therefore, genetic manipulation of crop plants with genes encoding enzymes of polyamine biosynthetic pathways may provide better stress tolerance to crop plants. Furthermore, the exogenous application of PAs is also another option for increasing the stress tolerance potential in plants. Here, we have described the synthesis and role of various polyamines in abiotic stress tolerance in plants. PMID:20592804

  3. Supervisory Control of Underwater Telemanipulators: Design and Experiment.

    DTIC Science & Technology

    1982-08-30

    control ........ 61 3.6 Descriptions for process level control ......... 67 Chapter 4 The design of a software man-machine interface...different types of human oriented interfaces for achieving such control will be presented. 4. The design of a new man-machine interface system intended 11...under the direction of a human operator [6]. Supervisory control fits on a continuum between manually controlled

  4. The interaction between abiotic photodegradation and microbial decomposition under ultraviolet radiation.

    PubMed

    Wang, Jing; Liu, Lingli; Wang, Xin; Chen, Yiwei

    2015-05-01

    Elevated ultraviolet (UV) radiation has been demonstrated to stimulate litter decomposition. Despite years of research, it is still not fully understood whether the acceleration in litter degradation is primarily attributed to abiotic photodegradation or the combined effects of abiotic photodegradation and microbial decomposition. In this study, we used meta-analysis to synthesize photodegradation studies and compared the effects of UV radiation on litter decomposition between abiotic and biotic conditions. We also conducted a microcosm experiment to assess the effects of UV radiation on litter biodegradability and microbial activity. Overall, our meta-analysis found that under abiotic photodegradation, UV radiation reduced the remaining litter mass by 1.44% (95% CI: 0.85% to 2.08%), did not affect the remaining lignin and increased the dissolved organic carbon (DOC) concentration by 14.01% (1.49-23.67%). Under combined abiotic photodegradation and microbial decomposition, UV radiation reduced the remaining litter mass and lignin by 1.60% (0.04-3.58%) and 16.07% (9.27-24.23%), respectively, but did not alter DOC concentration. UV radiation had no significant impact on soil microbial biomass carbon (MBC), but it reduced microbial respiration by 44.91% (2.26-78.62%) and altered the composition of the microbial community. In addition, UV radiation reduced nitrogen (N) immobilization by 19.44% (4.77-37.92%). Our microcosm experiment further indicated that DOC concentration and the amount of respired C in UV-treated litter increased with UV exposure time, suggesting that longer UV exposure resulted in greater biodegradability. Overall, our study suggested that UV exposure could increase litter biodegradability by increasing the microbial accessibility of lignin, as well as the labile carbon supply to microbes. However, the remaining litter mass was not different between the abiotic and biotic conditions, most likely because the positive effect of UV radiation on litter

  5. Oxygen and sulfur isotope systematics of sulfate produced during abiotic and bacterial oxidation of sphalerite and elemental sulfur

    USGS Publications Warehouse

    Balci, N.; Mayer, B.; Shanks, Wayne C.; Mandernack, K.W.

    2012-01-01

    Studies of metal sulfide oxidation in acid mine drainage (AMD) systems have primarily focused on pyrite oxidation, although acid soluble sulfides (e.g., ZnS) are predominantly responsible for the release of toxic metals. We conducted a series of biological and abiotic laboratory oxidation experiments with pure and Fe-bearing sphalerite (ZnS & Zn 0.88Fe 0.12S), respectively, in order to better understand the effects of sulfide mineralogy and associated biogeochemical controls of oxidation on the resultant ?? 34S and ?? 18O values of the sulfate produced. The minerals were incubated in the presence and absence of Acidithiobacillus ferrooxidans at an initial solution pH of 3 and with water of varying ?? 18O values to determine the relative contributions of H 2O-derived and O 2-derived oxygen in the newly formed sulfate. Experiments were conducted under aerobic and anaerobic conditions using O 2 and Fe(III) aq as the oxidants, respectively. Aerobic incubations with A. ferrooxidans, and S o as the sole energy source were also conducted. The ??34SSO4 values from both the biological and abiotic oxidation of ZnS and ZnS Fe by Fe(III) aq produced sulfur isotope fractionations (??34SSO4-ZnS) of up to -2.6???, suggesting the accumulation of sulfur intermediates during incomplete oxidation of the sulfide. No significant sulfur isotope fractionation was observed from any of the aerobic experiments. Negative sulfur isotope enrichment factors (??34SSO4-ZnS) in AMD systems could reflect anaerobic, rather than aerobic pathways of oxidation. During the biological and abiotic oxidation of ZnS and ZnS Fe by Fe(III) aq all of the sulfate oxygen was derived from water, with measured ?? 18OSO 4-H 2O values of 8.2??0.2??? and 7.5??0.1???, respectively. Also, during the aerobic oxidation of ZnS Fe and S o by A. ferrooxidans, all of the sulfate oxygen was derived from water with similar measured ?? 18OSO 4-H 2O values of 8.1??0.1??? and 8.3??0.3???, respectively. During biological oxidation

  6. Computer Controlled Experiments Using the Interactive Microcomputer Peripheral.

    ERIC Educational Resources Information Center

    Howard, Edgar; Howard, Peter

    1985-01-01

    Describes the Interactive Microcomputer Peripheral (including major features, source, and current cost) and physics experiments using the instrument. The instrument can also be used for such purposes as counting, timing, and frequency measurement as well as for experiments in biology and experimental psychology. (JN)

  7. Feelings of Control: Contingency Determines Experience of Action

    ERIC Educational Resources Information Center

    Moore, James W.; Lagnado, David; Deal, Darvany C.; Haggard, Patrick

    2009-01-01

    The experience of causation is a pervasive product of the human mind. Moreover, the experience of causing an event alters subjective time: actions are perceived as temporally shifted towards their effects [Haggard, P., Clark, S., & Kalogeras, J. (2002). Voluntary action and conscious awareness. "Nature Neuroscience," 5(4), 382-385]. This temporal…

  8. Control and Stabilization: Making Millikan's Oil Drop Experiment Work

    ERIC Educational Resources Information Center

    Muller-Hill, Christoph; Heering, Peter

    2011-01-01

    Educational versions of Millikan's oil-drop experiment have frequently been criticized; suggestions for improvement either focus on technical innovations of the setup or on replacing the experiment by other approaches of familiarization, such as computer simulations. In our approach, we have analysed experimental procedures. In doing so, we were…

  9. The VICKSI computer control system, concept and operating experience

    NASA Astrophysics Data System (ADS)

    Busse, W.; Kluge, H.; Ziegler, K.

    1981-05-01

    A description of the VICKSI computer-control system is given. It uses CAMAC modules as unique interface between accelerator devices and the computer. Through a high degree of standardisation only seven different types of CAMAC modules are needed to control the accelerator facility. The idea of having one module control one accelerator device minimizes the cabling and also the software requirements. The operation of the control system has proved to be very reliable causing less than 2% down time of the facility.

  10. Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought.

    PubMed

    Almeida, Diego M; Almadanim, M Cecília; Lourenço, Tiago; Abreu, Isabel A; Saibo, Nelson J M; Oliveira, M Margarida

    2016-01-01

    Rice (Oryza sativa) is the primary source of food for more than half of the world population. Most rice varieties are severely injured by abiotic stresses, with strong social and economic impact. Understanding rice responses to stress may help breeding for more tolerant varieties. However, papers dealing with stress experiments often describe very different experimental designs, thus making comparisons difficult. The use of identical setups is the only way to generate comparable data. This chapter is organized into three sections, describing the experimental conditions established at the Genomics of Plant Stress (GPlantS) unit of ITQB to assess the response of rice plants to three different abiotic stresses--high salinity, cold stress, and drought. All sections include a detailed description of the materials and methodology, as well as useful notes gathered from the GPlantS team's experience. We use rice seedlings as plants at this stage show high sensitivity to abiotic stresses. For the salt and cold stress assays we use hydroponic cultures, while for the drought assay plants are grown in soil and subjected to water withholding. All setups enable visual score determination and are suitable for sample collection along the imposition of stress. The proposed methodologies are simple and affordable to implement in most labs, allowing the discrimination of several rice genotypes at the molecular and phenotypic level.

  11. Comparative study of biogenic and abiotic iron-containing materials

    NASA Astrophysics Data System (ADS)

    Cherkezova-Zheleva, Z.; Shopska, M.; Paneva, D.; Kovacheva, D.; Kadinov, G.; Mitov, I.

    2016-12-01

    Series of iron-based biogenic materials prepared by cultivation of Leptothrix group of bacteria in different feeding media ( Sphaerotilus-Leptothrix group of bacteria isolation medium, Adler, Lieske and silicon-iron-glucose-peptone) were studied. Control samples were obtained in the same conditions and procedures but the nutrition media were not infected with bacteria, i.e. they were sterile. Room and low temperature Mössbauer spectroscopy, powder X-ray diffraction (XRD), and infrared spectroscopy (IRS) were used to reveal the composition and physicochemical properties of biomass and respective control samples. Comparative analysis showed differences in their composition and dispersity of present phases. Sample composition included different ratio of nanodimensional iron oxyhydroxide and oxide phases. Relaxation phenomena such as superparamagnetism or collective magnetic excitation behaviour were registered for some of them. The experimental data showed that the biogenic materials were enriched in oxyhydroxides of high dispersion. Catalytic behaviour of a selected biomass and abiotic material were studied in the reaction of CO oxidation. In situ diffuse-reflectance (DR) IRS was used to monitor the phase transformations in the biomass and CO conversion.

  12. Primordial soup was edible: abiotically produced Miller-Urey mixture supports bacterial growth.

    PubMed

    Xie, Xueshu; Backman, Daniel; Lebedev, Albert T; Artaev, Viatcheslav B; Jiang, Liying; Ilag, Leopold L; Zubarev, Roman A

    2015-09-28

    Sixty years after the seminal Miller-Urey experiment that abiotically produced a mixture of racemized amino acids, we provide a definite proof that this primordial soup, when properly cooked, was edible for primitive organisms. Direct admixture of even small amounts of Miller-Urey mixture strongly inhibits E. coli bacteria growth due to the toxicity of abundant components, such as cyanides. However, these toxic compounds are both volatile and extremely reactive, while bacteria are highly capable of adaptation. Consequently, after bacterial adaptation to a mixture of the two most abundant abiotic amino acids, glycine and racemized alanine, dried and reconstituted MU soup was found to support bacterial growth and even accelerate it compared to a simple mixture of the two amino acids. Therefore, primordial Miller-Urey soup was perfectly suitable as a growth media for early life forms.

  13. Primordial soup was edible: abiotically produced Miller-Urey mixture supports bacterial growth

    PubMed Central

    Xie, Xueshu; Backman, Daniel; Lebedev, Albert T.; Artaev, Viatcheslav B.; Jiang, Liying; Ilag, Leopold L.; Zubarev, Roman A.

    2015-01-01

    Sixty years after the seminal Miller-Urey experiment that abiotically produced a mixture of racemized amino acids, we provide a definite proof that this primordial soup, when properly cooked, was edible for primitive organisms. Direct admixture of even small amounts of Miller-Urey mixture strongly inhibits E. coli bacteria growth due to the toxicity of abundant components, such as cyanides. However, these toxic compounds are both volatile and extremely reactive, while bacteria are highly capable of adaptation. Consequently, after bacterial adaptation to a mixture of the two most abundant abiotic amino acids, glycine and racemized alanine, dried and reconstituted MU soup was found to support bacterial growth and even accelerate it compared to a simple mixture of the two amino acids. Therefore, primordial Miller-Urey soup was perfectly suitable as a growth media for early life forms. PMID:26412575

  14. Abiotic stress responses in plant roots: a proteomics perspective

    PubMed Central

    Ghosh, Dipanjana; Xu, Jian

    2014-01-01

    Abiotic stress conditions adversely affect plant growth, resulting in significant decline in crop productivity. To mitigate and recover from the damaging effects of such adverse environmental conditions, plants have evolved various adaptive strategies at cellular and metabolic levels. Most of these strategies involve dynamic changes in protein abundance that can be best explored through proteomics. This review summarizes comparative proteomic studies conducted with roots of various plant species subjected to different abiotic stresses especially drought, salinity, flood, and cold. The main purpose of this article is to highlight and classify the protein level changes in abiotic stress response pathways specifically in plant roots. Shared as well as stressor-specific proteome signatures and adaptive mechanism(s) are simultaneously described. Such a comprehensive account will facilitate the design of genetic engineering strategies that enable the development of broad-spectrum abiotic stress-tolerant crops. PMID:24478786

  15. Next generation optical instruments and space experiment based on the LDEF thermal control surfaces experiment (S0069)

    NASA Technical Reports Server (NTRS)

    Wilkes, Donald R.

    1993-01-01

    The Thermal Control Surfaces Experiment (TCSE) was a successful experiment to study the effects of the space environment on thermal control surfaces using in-space optical properties measurements combined with post-flight analyses. The TCSE reflectometer performed well on the LDEF mission demonstrating that a portable compact integrating sphere spectroreflectometer can be built that is rugged and space rated. Since the retrieval of the TCSE package from space, several other instruments have evolved from its pioneering technologies. These are the Optical Properties Monitor (OPM), the Laboratory Portable SpectroReflectometer (LPSR), and the Space Portable SpectroReflectometer (SPSR). These instruments and experiment packages are described.

  16. Adaptive independent joint control of manipulators - Theory and experiment

    NASA Technical Reports Server (NTRS)

    Seraji, H.

    1988-01-01

    The author presents a simple decentralized adaptive control scheme for multijoint robot manipulators based on the independent joint control concept. The proposed control scheme for each joint consists of a PID (proportional integral and differential) feedback controller and a position-velocity-acceleration feedforward controller, both with adjustable gains. The static and dynamic couplings that exist between the joint motions are compensated by the adaptive independent joint controllers while ensuring trajectory tracking. The proposed scheme is implemented on a MicroVAX II computer for motion control of the first three joints of a PUMA 560 arm. Experimental results are presented to demonstrate that trajectory tracking is achieved despite strongly coupled, highly nonlinear joint dynamics. The results confirm that the proposed decentralized adaptive control of manipulators is feasible, in spite of strong interactions between joint motions. The control scheme presented is computationally very fast and is amenable to parallel processing implementation within a distributed computing architecture, where each joint is controlled independently by a simple algorithm on a dedicated microprocessor.

  17. Studies and Experiments in Structural Dynamics and Control

    DTIC Science & Technology

    2006-12-30

    11 Figure 6: Closed-Loop Responses Due To Statistical Controller Design......................... 13 Figure 7: Reaction Wheel and Thruster Torques Due...of self awareness. The outline of the research goes as follows. First, a brief modeling of satellite with reaction wheels and thrusters will be...7 shows the control action from both reaction wheel cluster and thrusters. It was noted that the action of statistical controller was large at the

  18. Expert system to control a fusion energy experiment

    SciTech Connect

    Johnson, R.R.; Canales, T.; Lager, D.

    1986-01-01

    This paper describes a system that automates neutral beam source conditioning. The system achieves this with artificial intelligence techniques by encoding the behavior of several experts as a set of if-then rules in an expert system. One of the functions of the expert system is to control an adaptive controller that, in turn, controls the neutral beam source. The architecture of the system is presented followed by a description of its performance.

  19. Flight experience with manually controlled unconventional aircraft motions

    NASA Technical Reports Server (NTRS)

    Barfield, A. F.

    1978-01-01

    A modified YF-16 aircraft was used to flight demonstrate decoupled modes under the USAF Fighter Control Configured Vehicle (CCV) Program. The direct force capabilities were used to implement seven manually controlled unconventional modes on the aircraft, allowing flat turns, decoupled normal acceleration control, independent longitudinal and lateral translations, uncoupled elevation and azimuth aiming, and blended direct lift. This paper describes the design, development, and flight testing of these control modes. The need for task-tailored mode authorities, gain-scheduling and selected closed-loop design is discussed.

  20. Out-reach in-space technology experiments program: Control of flexible robot manipulators in zero gravity, experiment definition phase

    NASA Technical Reports Server (NTRS)

    Phillips, Warren F.

    1989-01-01

    The results obtained show that it is possible to control light-weight robots with flexible links in a manner that produces good response time and does not induce unacceptable link vibrations. However, deflections induced by gravity cause large static position errors with such a control system. For this reason, it is not possible to use this control system for controlling motion in the direction of gravity. The control system does, on the other hand, have potential for use in space. However, in-space experiments will be needed to verify its applicability to robots moving in three dimensions.

  1. Coupled biotic-abiotic oxidation of organic matter by biogenic MnO_{2}

    NASA Astrophysics Data System (ADS)

    Gonzalez, Julia; Peña, Jasquelin

    2016-04-01

    Some reactive soil minerals are strongly implicated in stabilising organic matter. However, others can play an active role in the oxidation of organic molecules. In natural systems, layer-type manganese oxide minerals (MnO2) typically occur as biomineral assemblages consisting of mineral particles and microbial biomass. Both the mineral and biological fractions of the assemblage can be powerful oxidants of organic C. The biological compartment relies on a set of enzymes to drive oxidative transformations of reduced C-substrates, whereas MnO2 minerals are strong, less specific abiotic oxidants that are assumed to rely on interfacial interactions between C-substrates and the mineral surface. This project aims to understand the coupling between microbial C mineralization and abiotic C oxidation mediated by MnO2 in bacterial-MnO2 assemblages. Specifically, under conditions of high C turnover, microbial respiration can significantly alter local pH, dissolved oxygen and pool of available reductants, which may modify rates and mechanism of C oxidation by biotic and abiotic components. We first investigated changes in the solution chemistry of Pseudomonas putida suspensions exposed to varying concentrations of glucose, chosen to represent readily bioavailable substrates in soils. Glucose concentrations tested ranged between 0 and 5.5mM and changes in pH, dissolved oxygen and dissolved organic and inorganic carbon were tracked over 48h. We then combined literature review and wet-chemical experiments to compile the pH dependence of rates of organic substrate oxidation by MnO2, including glucose. Our results demonstrate a strong pH dependence for these abiotic reactions. In assemblages of P. putida - MnO2, kinetic limitations for abiotic C oxidation by MnO2 are overcome by changes in biogeochemical conditions that result from bacterial C metabolism. When extrapolated to a soil solution confronted to an input of fresh dissolved organic matter, bacterial C metabolism of the

  2. Evolution and Adaptation of Wild Emmer Wheat Populations to Biotic and Abiotic Stresses.

    PubMed

    Huang, Lin; Raats, Dina; Sela, Hanan; Klymiuk, Valentina; Lidzbarsky, Gabriel; Feng, Lihua; Krugman, Tamar; Fahima, Tzion

    2016-08-04

    The genetic bottlenecks associated with plant domestication and subsequent selection in man-made agroecosystems have limited the genetic diversity of modern crops and increased their vulnerability to environmental stresses. Wild emmer wheat, the tetraploid progenitor of domesticated wheat, distributed along a wide range of ecogeographical conditions in the Fertile Crescent, has valuable "left behind" adaptive diversity to multiple diseases and environmental stresses. The biotic and abiotic stress responses are conferred by series of genes and quantitative trait loci (QTLs) that control complex resistance pathways. The study of genetic diversity, genomic organization, expression profiles, protein structure and function of biotic and abiotic stress-resistance genes, and QTLs could shed light on the evolutionary history and adaptation mechanisms of wild emmer populations for their natural habitats. The continuous evolution and adaptation of wild emmer to the changing environment provide novel solutions that can contribute to safeguarding food for the rapidly growing human population.

  3. AFECS. Multi-Agent Framework for Experiment Control Systems

    SciTech Connect

    Vardan Gyurjyan; David Abbott; William Heyes; Edward Jastrzembski; Carl Timmer; Elliott Wolin

    2008-01-23

    AFECS is a pure Java based software framework for designing and implementing distributed control systems. AFECS creates a control system environment as a collection of software agents behaving as finite state machines. These agents can represent real entities, such as hardware devices, software tasks, or control subsystems. A special control oriented ontology language (COOL), based on RDFS (Resource Definition Framework Schema) is provided for control system description as well as for agent communication. AFECS agents can be distributed over a variety of platforms. Agents communicate with their associated physical components using range of communication protocols, including tcl-DP, cMsg (publish-subscribe communication system developed at Jefferson Lab), SNMP (simple network management protocol), EPICS channel access protocol and JDBC.

  4. [A field study of tundra plant litter decomposition rate via mass loss and carbon dioxide emission: the role of biotic and abiotic controls, biotope, season of year, and spatial-temporal scale].

    PubMed

    Pochikalov, A V; Karelin, D V

    2014-01-01

    Although many recently published original papers and reviews deal with plant matter decomposition rates and their controls, we are still very short in understanding of these processes in boreal and high latiude plant communities, especially in permafrost areas of our planet. First and foremost, this is holds true for winter period. Here, we present the results of 2-year field observations in south taiga and south shrub tundra ecosystems in European Russia. We pioneered in simultaneous application of two independent methods: classic mass loss estimation by litter-bag technique, and direct measurement of CO2 emission (respiration) of the same litter bags with different types of dead plant matter. Such an approach let us to reconstruct intra-seasonal dynamics of decomposition rates of the main tundra litter fractions with high temporal resolution, to estimate the partial role of different seasons and defragmentation in the process of plant matter decomposition, and to determine its factors under different temporal scale.

  5. MICROSCALE METABOLIC, REDOX AND ABIOTIC REACTIONS IN HANFORD 300 AREA SUBSURFACE SEDIMENTS

    SciTech Connect

    Beyenal, Haluk; McLEan, Jeff; Majors, Paul; Fredrickson, Jim

    2013-11-14

    The Hanford 300 Area is a unique site due to periodic hydrologic influence of river water resulting in changes in groundwater elevation and flow direction. This area is also highly subject to uranium remobilization, the source of which is currently believed to be the region at the base of the vadose zone that is subject to period saturation due to the changes in the water levels in the Columbia River. We found that microbial processes and redox and abiotic reactions which operate at the microscale were critical to understanding factors controlling the macroscopic fate and transport of contaminants in the subsurface. The combined laboratory and field research showed how microscale conditions control uranium mobility and how biotic, abiotic and redox reactions relate to each other. Our findings extended the current knowledge to examine U(VI) reduction and immobilization using natural 300 Area communities as well as selected model organisms on redox-sensitive and redox-insensitive minerals. Using innovative techniques developed specifically to probe biogeochemical processes at the microscale, our research expanded our current understanding of the roles played by mineral surfaces, bacterial competition, and local biotic, abiotic and redox reaction rates on the reduction and immobilization of uranium.

  6. Semiochemicals from ex situ abiotically stressed cactus tissue: a contributing role of fungal spores?

    PubMed

    Beck, John J; Baig, Nausheena; Cook, Daniel; Mahoney, Noreen E; Marsico, Travis D

    2014-12-24

    Semiochemicals play a central role in communication between plants and insects, such as signaling the location of a suitable host. Fungi on host plants can also play an influential role in communicating certain plant vulnerabilities to an insect. The spiroketal conophthorin is an important semiochemical produced by developing fungal spores. Spiroketals are also used as signals for scolytid communication. Plants and fungi are known to emit varying volatile profiles under biotic and abiotic stress. This paper reports distinctive temporal-volatile profiles from three abiotic treatments, room temperature (control), -15 °C (cold), and -15 °C to room temperature (shock), of cactus tissue plugs. Volatiles from the three treatments included monoterpenes from control plugs, compounds of varying classes and origin at later stages for cold plugs, and known semiochemicals, including spiroketals, at later stages for shock plugs. The results highlight several important findings: a unique tissue source of the spiroketals; abiotic cold-shock stress is indicated as the cause of spiroketal production; and, given previous findings of spirogenesis, fungal spore involvement is a probable biosynthetic origin of the spiroketals. These findings suggest an important role of fungal volatiles as signaling plant vulnerability to insects.

  7. ROS-mediated abiotic stress-induced programmed cell death in plants

    PubMed Central

    Petrov, Veselin; Hille, Jacques; Mueller-Roeber, Bernd; Gechev, Tsanko S.

    2015-01-01

    During the course of their ontogenesis plants are continuously exposed to a large variety of abiotic stress factors which can damage tissues and jeopardize the survival of the organism unless properly countered. While animals can simply escape and thus evade stressors, plants as sessile organisms have developed complex strategies to withstand them. When the intensity of a detrimental factor is high, one of the defense programs employed by plants is the induction of programmed cell death (PCD). This is an active, genetically controlled process which is initiated to isolate and remove damaged tissues thereby ensuring the survival of the organism. The mechanism of PCD induction usually includes an increase in the levels of reactive oxygen species (ROS) which are utilized as mediators of the stress signal. Abiotic stress-induced PCD is not only a process of fundamental biological importance, but also of considerable interest to agricultural practice as it has the potential to significantly influence crop yield. Therefore, numerous scientific enterprises have focused on elucidating the mechanisms leading to and controlling PCD in response to adverse conditions in plants. This knowledge may help develop novel strategies to obtain more resilient crop varieties with improved tolerance and enhanced productivity. The aim of the present review is to summarize the recent advances in research on ROS-induced PCD related to abiotic stress and the role of the organelles in the process. PMID:25741354

  8. Progress in Controlled In Situ Ocean Acidification Experiments

    NASA Astrophysics Data System (ADS)

    Brewer, Peter; Kirkwood, William; Gattuso, Jean-Pierre

    2013-04-01

    Ocean acidification is widely recognized as a significant climate-related oceanic threat, not only independently but also in connection with other oceanic stressors, including warming and deoxygenation. Recent work shows that ocean acidification will negatively affect processes such as calcification of most species, including reef-building corals, and could also cause diminished fish sensory ability and respiratory stress. However, almost all of these findings result from short-term experiments on organisms in laboratory aquaria. But how can scientists perform long-term in situ experiments that may confirm, or modify, conclusions drawn from laboratory experiments? With funding from the BNP Paribas Foundation, the xFOCE workshop brought together a group of 20 scientists and engineers to examine this.

  9. Current Laminar Flow Control Experiments at NASA Dryden

    NASA Technical Reports Server (NTRS)

    Bowers, Al

    2010-01-01

    An experiment to demonstrate laminar flow over the swept wing of a subsonic transport is being developed. Discrete Roughness Elements are being used to maintain laminar flow over a substantial portion of a wing glove. This passive laminar flow technology has only come to be recognized as a significant player in airliner drag reduction in the last few years. NASA is implementing this experiment and is planning to demonstrate this technology at full-scale Bight cruise conditions of a small-to-medium airliner.

  10. Identification of Cassava MicroRNAs under Abiotic Stress

    PubMed Central

    Ballén-Taborda, Carolina; Plata, Germán; Ayling, Sarah; Rodríguez-Zapata, Fausto; Tohme, Joe

    2013-01-01

    The study of microRNAs (miRNAs) in plants has gained significant attention in recent years due to their regulatory role during development and in response to biotic and abiotic stresses. Although cassava (Manihot esculenta Crantz) is tolerant to drought and other adverse conditions, most cassava miRNAs have been predicted using bioinformatics alone or through sequencing of plants challenged by biotic stress. Here, we use high-throughput sequencing and different bioinformatics methods to identify potential cassava miRNAs expressed in different tissues subject to heat and drought conditions. We identified 60 miRNAs conserved in other plant species and 821 potential cassava-specific miRNAs. We also predicted 134 and 1002 potential target genes for these two sets of sequences. Using real time PCR, we verified the condition-specific expression of 5 cassava small RNAs relative to a non-stress control. We also found, using publicly available expression data, a significantly lower expression of the predicted target genes of conserved and nonconserved miRNAs under drought stress compared to other cassava genes. Gene Ontology enrichment analysis along with condition specific expression of predicted miRNA targets, allowed us to identify several interesting miRNAs which may play a role in stress-induced posttranscriptional regulation in cassava and other plants. PMID:24328029

  11. Active Noise Control Experiments using Sound Energy Flu

    NASA Astrophysics Data System (ADS)

    Krause, Uli

    2015-03-01

    This paper reports on the latest results concerning the active noise control approach using net flow of acoustic energy. The test set-up consists of two loudspeakers simulating the engine noise and two smaller loudspeakers which belong to the active noise system. The system is completed by two acceleration sensors and one microphone per loudspeaker. The microphones are located in the near sound field of the loudspeakers. The control algorithm including the update equation of the feed-forward controller is introduced. Numerical simulations are performed with a comparison to a state of the art method minimising the radiated sound power. The proposed approach is experimentally validated.

  12. The Promoter of AtUSP Is Co-regulated by Phytohormones and Abiotic Stresses in Arabidopsis thaliana

    PubMed Central

    Bhuria, Monika; Goel, Parul; Kumar, Sanjay; Singh, Anil K.

    2016-01-01

    Universal stress proteins (USPs) are known to be expressed in response to various abiotic stresses in a wide variety of organisms, such as bacteria, archaebacteria, protists, algae, fungi, plants, and animals. However, in plants, biological function of most of the USPs still remains obscure. In the present study, Arabidopsis USP gene (AtUSP) showed induction in response to abscisic acid (ABA) and various abiotic stresses viz. heat, dehydration, salt, osmotic, and cold stresses. Additionally, in silico analysis of AtUSP promoter identified several cis-elements responsive to phytohormones and abiotic stresses such as ABRE, ERE, DRE, and HSE, etc. To functionally validate the AtUSP promoter, the 1115 bp region of promoter was characterized under phytohormone and abiotic stress treatments. Deletion analysis of promoter was carried out by cloning the full length promoter (D0) and its three 5′ deletion derivatives, D1 (964 bp), D2 (660 bp), and D3 (503 bp) upstream of the β-glucuronidase (GUS) reporter gene, which were then stably transformed in Arabidopsis plants. The AtUSP promoter (D0) showed minimal activity under non-stress conditions which was enhanced in response to phytohormone treatments (ABA and ACC) and abiotic stresses such as dehydration, heat, cold, salt, and osmotic stresses. The seedlings harboring D1 and D2 deletion fragments showed constitutive GUS expression even under control condition with increased activity almost under all the treatments. However, D3 seedlings exhibited complete loss of activity under control condition with induction under ACC treatment, dehydration, heat, oxidative, salt, and osmotic stresses. Thus, present study clearly showed that AtUSP promoter is highly inducible by phytohormones and multiple abiotic stresses and it can be exploited as stress inducible promoter to generate multi-stress tolerant crops with minimal effects on their other important traits. PMID:28083000

  13. Inventory Control. Easily Made Electronic Device for Conductivity Experiments.

    ERIC Educational Resources Information Center

    Gadek, Frank J.

    1987-01-01

    Describes how to construct an electronic device to be used in conductivity experiments using a 35 millimeter film canister, nine volt battery replacement snaps, a 200-300 ohm resistor, and a light-emitting diode. Provides a diagram and photographs of the device. (TW)

  14. A Nonlinear, Multiinput, Multioutput Process Control Laboratory Experiment

    ERIC Educational Resources Information Center

    Young, Brent R.; van der Lee, James H.; Svrcek, William Y.

    2006-01-01

    Experience in using a user-friendly software, Mathcad, in the undergraduate chemical reaction engineering course is discussed. Example problems considered for illustration deal with simultaneous solution of linear algebraic equations (kinetic parameter estimation), nonlinear algebraic equations (equilibrium calculations for multiple reactions and…

  15. A Controlled Drug-Delivery Experiment Using Alginate Beads

    ERIC Educational Resources Information Center

    Farrell, Stephanie; Vernengo, Jennifer

    2012-01-01

    This paper describes a simple, cost-effective experiment which introduces students to drug delivery and modeling using alginate beads. Students produce calcium alginate beads loaded with drug and measure the rate of release from the beads for systems having different stir rates, geometries, extents of cross-linking, and drug molecular weight.…

  16. Interactions between abiotic constraint, propagule pressure, and biotic resistance regulate plant invasion.

    PubMed

    Byun, Chaeho; de Blois, Sylvie; Brisson, Jacques

    2015-05-01

    With multiple species introductions and rapid global changes, there is a need for comprehensive invasion models that can predict community responses. Evidence suggests that abiotic constraint, propagule pressure, and biotic resistance of resident species each determine plant invasion success, yet their interactions are rarely tested. To understand these interactions, we conducted community assembly experiments simulating situations in which seeds of the invasive grass species Phragmites australis (Poaceae) land on bare soil along with seeds of resident wetland plant species. We used structural equation models to measure both direct abiotic constraint (here moist vs. flooded conditions) on invasion success and indirect constraint on the abundance and, therefore, biotic resistance of resident plant species. We also evaluated how propagule supply of P. australis interacts with the biotic resistance of resident species during invasion. We observed that flooding always directly reduced invasion success but had a synergistic or antagonistic effect on biotic resistance depending on the resident species involved. Biotic resistance of the most diverse resident species mixture remained strong even when abiotic conditions changed. Biotic resistance was also extremely effective under low propagule pressure of the invader. Moreover, the presence of a dense resident plant cover appeared to lower the threshold at which invasion success became stable even when propagule supply increased. Our study not only provides an analytical framework to quantify the effect of multiple interactions relevant to community assembly and species invasion, but it also proposes guidelines for innovative invasion management strategies based on a sound understanding of ecological processes.

  17. Polyamines and abiotic stress in plants: a complex relationship1

    PubMed Central

    Minocha, Rakesh; Majumdar, Rajtilak; Minocha, Subhash C.

    2014-01-01

    The physiological relationship between abiotic stress in plants and polyamines was reported more than 40 years ago. Ever since there has been a debate as to whether increased polyamines protect plants against abiotic stress (e.g., due to their ability to deal with oxidative radicals) or cause damage to them (perhaps due to hydrogen peroxide produced by their catabolism). The observation that cellular polyamines are typically elevated in plants under both short-term as well as long-term abiotic stress conditions is consistent with the possibility of their dual effects, i.e., being protectors from as well as perpetrators of stress damage to the cells. The observed increase in tolerance of plants to abiotic stress when their cellular contents are elevated by either exogenous treatment with polyamines or through genetic engineering with genes encoding polyamine biosynthetic enzymes is indicative of a protective role for them. However, through their catabolic production of hydrogen peroxide and acrolein, both strong oxidizers, they can potentially be the cause of cellular harm during stress. In fact, somewhat enigmatic but strong positive relationship between abiotic stress and foliar polyamines has been proposed as a potential biochemical marker of persistent environmental stress in forest trees in which phenotypic symptoms of stress are not yet visible. Such markers may help forewarn forest managers to undertake amelioration strategies before the appearance of visual symptoms of stress and damage at which stage it is often too late for implementing strategies for stress remediation and reversal of damage. This review provides a comprehensive and critical evaluation of the published literature on interactions between abiotic stress and polyamines in plants, and examines the experimental strategies used to understand the functional significance of this relationship with the aim of improving plant productivity, especially under conditions of abiotic stress. PMID:24847338

  18. Study of thermal control systems for orbiting power systems. Materials experiment carrier thermal control system study

    NASA Technical Reports Server (NTRS)

    Fleming, M. L.

    1980-01-01

    Four possible arrangements of the materials experiment carrier (MEC) and power system (PS) thermal control loops were defined which would provide one kW of heat rejection for each kW of power to the MEC payload. These arrangements were compared to the baseline reference concept which provides only 16 kW heat rejection to show the cost of obtaining symmetry in terms of dollars, weight, complexity, growth potential, ease of integration, technology and total launch weight. The results of these comparisons was that the concept which splits the PS thermal control loop into two systems, one to reject PS waste heat and one payload waste heat, appeared favorable. The fluid selection study resulted in recommendation of FC72 as the MEC heat transport fluid based on the thermal and physical characteristics. The coatings reviewed indicated anodized and alodine treated aluminum surfaces or silver teflon are the best choices for the MEC vehicle where durability is an important factor. For high temperature radiators silver teflon or zinc orthotitanate are recommended choices.

  19. Radiopurity control in the NEXT-100 double beta decay experiment

    NASA Astrophysics Data System (ADS)

    Álvarez, V.; Bandac, I.; Bettini, A.; Borges, F. I. G. M.; Cárcel, S.; Castel, J.; Cebrián, S.; Cervera, A.; Conde, C. A. N.; Dafni, T.; Dias, T. H. V. T.; Díaz, J.; Egorov, M.; Esteve, R.; Evtoukhovitch, P.; Fernandes, L. M. P.; Ferrario, P.; Ferreira, A. L.; Freitas, E. D. C.; Gehman, V. M.; Gil, A.; Goldschmidt, A.; Gómez, H.; Gómez-Cadenas, J. J.; González-Díaz, D.; Gutiérrez, R. M.; Hauptman, J.; Hernando Morata, J. A.; Herrera, D. C.; Iguaz, F. J.; Irastorza, I. G.; Jinete, M. A.; Labarga, L.; Laing, A.; Liubarsky, I.; Lopes, J. A. M.; Lorca, D.; Losada, M.; Luzón, G.; Marí, A.; Martín-Albo, J.; Martínez, A.; Miller, T.; Moiseenko, A.; Monrabal, F.; Monteiro, C. M. B.; Mora, F. J.; Moutinho, L. M.; Muñoz Vidal, J.; da Luz, H. Natal; Navarro, G.; Nebot-Guinot, M.; Nygren, D.; Oliveira, C. A. B.; de Solórzano, A. Ortiz; Palma, R.; Pérez, J.; Pérez Aparicio, J. L.; Renner, J.; Ripoll, L.; Rodríguez, A.; Rodríguez, J.; Santos, F. P.; dos Santos, J. M. F.; Segui, L.; Serra, L.; Shuman, D.; Simón, A.; Sofka, C.; Sorel, M.; Toledo, J. F.; Tomás, A.; Torrent, J.; Tsamalaidze, Z.; Vázquez, D.; Veloso, J. F. C. A.; Villar, J. A.; Webb, R. C.; White, J. T.; Yahlali, N.

    2013-08-01

    An extensive material screening and selection process is underway in the construction of the "Neutrino Experiment with a Xenon TPC" (NEXT), intended to investigate neutrinoless double beta decay using a high-pressure xenon gas TPC filled with 100 kg of Xe enriched in 136Xe. Determination of the radiopurity levels of the materials is based on gamma-ray spectroscopy using ultra-low background germanium detectors at the Laboratorio Subterráneo de Canfranc (Spain) and also on Glow Discharge Mass Spectrometry. Materials to be used in the shielding, pressure vessel, electroluminescence and high voltage components and energy and tracking readout planes have been already taken into consideration. The measurements carried out are presented, describing the techniques and equipment used, and the results obtained are shown, discussing their implications for the NEXT experiment.

  20. Control and stabilization: making Millikan's oil drop experiment work

    NASA Astrophysics Data System (ADS)

    Müller-Hill, Christoph; Heering, Peter

    2011-09-01

    Educational versions of Millikan's oil-drop experiment have frequently been criticized; suggestions for improvement either focus on technical innovations of the setup or on replacing the experiment by other approaches of familiarization, such as computer simulations. In our approach, we have analysed experimental procedures. In doing so, we were able to identify several sources of error and took measures to minimize their influence. At the same time, we attempted to minimize the standard deviation of each individual series of measurements. Our paper describes how we developed criteria which helped to stabilize the data produced in the following series of measurements. The final series of measurements results in data which demonstrate the atomic structure of electricity and enable a demonstration of the elementary charge.

  1. Radiopurity control in the NEXT-100 double beta decay experiment

    SciTech Connect

    Álvarez, V.; Cárcel, S.; Cervera, A.; Díaz, J.; Ferrario, P.; Gil, A.; Gómez-Cadenas, J. J.; Laing, A.; Liubarsky, I.; Lorca, D.; Martín-Albo, J.; Martínez, A.; Monrabal, F.; Muñoz Vidal, J.; Nebot-Guinot, M.; Rodríguez, J.; Serra, L.; Simón, A.; Sofka, C.; Sorel, M.; and others

    2013-08-08

    An extensive material screening and selection process is underway in the construction of the 'Neutrino Experiment with a Xenon TPC' (NEXT), intended to investigate neutrinoless double beta decay using a high-pressure xenon gas TPC filled with 100 kg of Xe enriched in {sup 136}Xe. Determination of the radiopurity levels of the materials is based on gamma-ray spectroscopy using ultra-low background germanium detectors at the Laboratorio Subterráneo de Canfranc (Spain) and also on Glow Discharge Mass Spectrometry. Materials to be used in the shielding, pressure vessel, electroluminescence and high voltage components and energy and tracking readout planes have been already taken into consideration. The measurements carried out are presented, describing the techniques and equipment used, and the results obtained are shown, discussing their implications for the NEXT experiment.

  2. Aerodynamics of ski jumping flight and its control: I. Experiments

    NASA Astrophysics Data System (ADS)

    Jung, Daehan; Bang, Kyeongtae; Kim, Heesu; Ahn, Eunhye; Choi, Haecheon

    2015-11-01

    In a ski jumping competition, it is essential to analyze the effect of various posture parameters of a ski jumper to achieve a longer flight distance. For this purpose, we construct a model of a ski jumper by using three-dimensional surface data obtained by scanning a ski jumper's body (Mr. Chil-Ku Kang, member of the Korean national team). An experiment on this model is conducted in a wind tunnel. We consider four posture parameters (forward leaning angle, ski opening angle, ski rolling angle, and ski spacing) and measure the drag and lift forces for various flight postures at various angles of attack (α = 0° - 40°) and Reynolds numbers (Re = 5.4 × 105 - 1.6 × 106) based on the length of the jump ski. Then, we derive optimum values of posture parameters for maximum lift-to-drag ratio using a response surface method. We also conduct a full-scale wind tunnel experiment with members of the Korean national team and confirm the results obtained from the experiment on the model. Supported by the NRF program (2014M3C1B1033848).

  3. Experiments in identification and control of flexible-link manipulators

    NASA Technical Reports Server (NTRS)

    Yurkovich, Stephen; Tzes, Anthony P.; Pacheco, Fernando E.

    1989-01-01

    Interest in the study of flexible-link manipulators for space-based applications has risen strongly in recent years. Moreover, numerous experimental results have appeared for the various problems in the modeling, identification and control of such systems. Nevertheless, relatively little literature has appeared involving laboratory verification of tuning controllers for certain types of realistic flexible-link manipulators. Specifically flexible-link manipulators which are required to maintain endpoint accuracy while manipulating loads that are possibly unknown and varying as they undergo disturbance effects from the environment and workspace. Endpoint position control of flexible-link manipulators in these areas are discussed, with laboratory setups consisting of one and two-link manipulators.

  4. Experience with ISO quality control in assisted reproductive technology.

    PubMed

    Alper, Michael M

    2013-12-01

    Assisted reproductive technology (ART) programs are complex organizations requiring the integration of multiple disciplines. ISO 9001:2008 is a quality management system that is readily adaptable to an ART program. The value that ISO brings to the entire organization includes control of documents, clear delineation of responsibilities of staff members, documentation of the numerous processes and procedures, improvement in tracking and reducing errors, and overall better control of systems. A quality ART program sets quality objectives and monitors their progress. ISO provides a sense of transparency within the organization and clearer understanding of how service is provided to patients. Most importantly, ISO provides the framework to allow for continual improvement.

  5. Apollo experience report: Crew station integration. Volume 2: Crew station displays and controls

    NASA Technical Reports Server (NTRS)

    Langdoc, W. A.; Nassman, D. A.

    1975-01-01

    The functional requirements for the Apollo displays and controls system are presented. The configuration of the displays, controls, and panels for both the command module and the lunar module are described, and the design development and operational experience of the displays and controls system are discussed. Pertinent recommendations for future displays and controls system design efforts are made.

  6. PID Controller Settings Based on a Transient Response Experiment

    ERIC Educational Resources Information Center

    Silva, Carlos M.; Lito, Patricia F.; Neves, Patricia S.; Da Silva, Francisco A.

    2008-01-01

    An experimental work on controller tuning for chemical engineering undergraduate students is proposed using a small heat exchange unit. Based upon process reaction curves in open-loop configuration, system gain and time constant are determined for first order model with time delay with excellent accuracy. Afterwards students calculate PID…

  7. Second Careers: The Air Traffic Controller Experience and Beyond.

    ERIC Educational Resources Information Center

    Batten, Michael D.

    1978-01-01

    Second careers are examined from an organizational viewpoint, and new directions for education-work policy, suggested by a unique second career program of the Federal Aviation Administration for air traffic controllers, are explored. Focus is on age, organizational and training factors, and community involvement. (Author/JMD)

  8. Apollo experience report: Lunar module display and control subsystem

    NASA Technical Reports Server (NTRS)

    Farkas, A. J.

    1972-01-01

    The lunar module display and control subsystem equipment is described with emphasis on major problems and their solutions. Included in the discussion of each item is a description of what the item does and how the item is constructed. The development, hardware history, and testing for each item are also presented.

  9. Designing Hospital for better Infection Control: an Experience.

    PubMed

    Rao, Skm

    2004-01-01

    The physical design of hospital is an essential component of a hospital's infection control strategy, incorporating infection control issues to minimise the risk of infection transmission. Hospital design therefore, needs to consider the separation of dirty and clean areas, adequate ventilation, lighting and storage facilities and design of patient accommodation areas, including adequate number of wash hand basins and single bed facilities. A 250 bed general hospital was planned keeping in view structural and design elements necessary for success of a good infection control programme. Various National and International Standards like BSI recommendations, JCAHO, IC Standards, DHSS, ASHRAE, AIA and OSHA were studied and compared with our planning parameters. Planning of ward unit, ICU, Operation theatre and Isolation wards were especially reviewed in the light of recent knowledge available in the field of hospital acquired infection and modifications were carried out. Need for effective identification of potential infections, risks in the design of a hospital were stressed. Engineering controls required to reduce the concentration of infectious droplet nuclei in the air and prevention of transmission of disease were highlighted.

  10. Plant cell organelle proteomics in response to abiotic stress.

    PubMed

    Hossain, Zahed; Nouri, Mohammad-Zaman; Komatsu, Setsuko

    2012-01-01

    Proteomics is one of the finest molecular techniques extensively being used for the study of protein profiling of a given plant species experiencing stressed conditions. Plants respond to a stress by alteration in the pattern of protein expression, either by up-regulating of the existing protein pool or by the synthesizing novel proteins primarily associated with plants antioxidative defense mechanism. Improved protein extraction protocols and advance techniques for identification of novel proteins have been standardized in different plant species at both cellular and whole plant level for better understanding of abiotic stress sensing and intracellular stress signal transduction mechanisms. In contrast, an in-depth proteome study of subcellular organelles could generate much detail information about the intrinsic mechanism of stress response as it correlates the possible relationship between the protein abundance and plant stress tolerance. Although a wealth of reviews devoted to plant proteomics are available, review articles dedicated to plant cell organelle proteins response under abiotic stress are very scanty. In the present review, an attempt has been made to summarize all significant contributions related to abiotic stresses and their impacts on organelle proteomes for better understanding of plants abiotic stress tolerance mechanism at protein level. This review will not only provide new insights into the plants stress response mechanisms, which are necessary for future development of genetically engineered stress tolerant crop plants for the benefit of humankind, but will also highlight the importance of studying changes in protein abundance within the cell organelles in response to abiotic stress.

  11. Impact of Post-Translational Modifications of Crop Proteins under Abiotic Stress

    PubMed Central

    Hashiguchi, Akiko; Komatsu, Setsuko

    2016-01-01

    The efficiency of stress-induced adaptive responses of plants depends on intricate coordination of multiple signal transduction pathways that act coordinately or, in some cases, antagonistically. Protein post-translational modifications (PTMs) can regulate protein activity and localization as well as protein–protein interactions in numerous cellular processes, thus leading to elaborate regulation of plant responses to various external stimuli. Understanding responses of crop plants under field conditions is crucial to design novel stress-tolerant cultivars that maintain robust homeostasis even under extreme conditions. In this review, proteomic studies of PTMs in crops are summarized. Although the research on the roles of crop PTMs in regulating stress response mechanisms is still in its early stage, several novel insights have been retrieved so far. This review covers techniques for detection of PTMs in plants, representative PTMs in plants under abiotic stress, and how PTMs control functions of representative proteins. In addition, because PTMs under abiotic stresses are well described in soybeans under submergence, recent findings in PTMs of soybean proteins under flooding stress are introduced. This review provides information on advances in PTM study in relation to plant adaptations to abiotic stresses, underlining the importance of PTM study to ensure adequate agricultural production in the future. PMID:28248251

  12. Recent Advances in Utilizing Transcription Factors to Improve Plant Abiotic Stress Tolerance by Transgenic Technology

    PubMed Central

    Wang, Hongyan; Wang, Honglei; Shao, Hongbo; Tang, Xiaoli

    2016-01-01

    Agricultural production and quality are adversely affected by various abiotic stresses worldwide and this will be exacerbated by the deterioration of global climate. To feed a growing world population, it is very urgent to breed stress-tolerant crops with higher yields and improved qualities against multiple environmental stresses. Since conventional breeding approaches had marginal success due to the complexity of stress tolerance traits, the transgenic approach is now being popularly used to breed stress-tolerant crops. So identifying and characterizing the critical genes involved in plant stress responses is an essential prerequisite for engineering stress-tolerant crops. Far beyond the manipulation of single functional gene, engineering certain regulatory genes has emerged as an effective strategy now for controlling the expression of many stress-responsive genes. Transcription factors (TFs) are good candidates for genetic engineering to breed stress-tolerant crop because of their role as master regulators of many stress-responsive genes. Many TFs belonging to families AP2/EREBP, MYB, WRKY, NAC, bZIP have been found to be involved in various abiotic stresses and some TF genes have also been engineered to improve stress tolerance in model and crop plants. In this review, we take five large families of TFs as examples and review the recent progress of TFs involved in plant abiotic stress responses and their potential utilization to improve multiple stress tolerance of crops in the field conditions. PMID:26904044

  13. Natural variation for gene expression responses to abiotic stress in maize.

    PubMed

    Waters, Amanda J; Makarevitch, Irina; Noshay, Jaclyn; Burghardt, Liana T; Hirsch, Candice N; Hirsch, Cory D; Springer, Nathan M

    2017-02-01

    Plants respond to abiotic stress through a variety of physiological, biochemical, and transcriptional mechanisms. Many genes exhibit altered levels of expression in response to abiotic stress, which requires concerted action of both cis- and trans-regulatory features. In order to study the variability in transcriptome response to abiotic stress, RNA sequencing was performed using 14-day-old maize seedlings of inbreds B73, Mo17, Oh43, PH207 and B37 under control, cold and heat conditions. Large numbers of genes that responded differentially to stress between parental inbred lines were identified. RNA sequencing was also performed on similar tissues of the F1 hybrids produced by crossing B73 and each of the three other inbred lines. By evaluating allele-specific transcript abundance in the F1 hybrids, we were able to measure the abundance of cis- and trans-regulatory variation between genotypes for both steady-state and stress-responsive expression differences. Although examples of trans-regulatory variation were observed, cis-regulatory variation was more common for both steady-state and stress-responsive expression differences. The genes with cis-allelic variation for response to cold or heat stress provided an opportunity to study the basis for regulatory diversity.

  14. Natural variation in abiotic stress responsive gene expression and local adaptation to climate in Arabidopsis thaliana.

    PubMed

    Lasky, Jesse R; Des Marais, David L; Lowry, David B; Povolotskaya, Inna; McKay, John K; Richards, James H; Keitt, Timothy H; Juenger, Thomas E

    2014-09-01

    Gene expression varies widely in natural populations, yet the proximate and ultimate causes of this variation are poorly known. Understanding how variation in gene expression affects abiotic stress tolerance, fitness, and adaptation is central to the field of evolutionary genetics. We tested the hypothesis that genes with natural genetic variation in their expression responses to abiotic stress are likely to be involved in local adaptation to climate in Arabidopsis thaliana. Specifically, we compared genes with consistent expression responses to environmental stress (expression stress responsive, "eSR") to genes with genetically variable responses to abiotic stress (expression genotype-by-environment interaction, "eGEI"). We found that on average genes that exhibited eGEI in response to drought or cold had greater polymorphism in promoter regions and stronger associations with climate than those of eSR genes or genomic controls. We also found that transcription factor binding sites known to respond to environmental stressors, especially abscisic acid responsive elements, showed significantly higher polymorphism in drought eGEI genes in comparison to eSR genes. By contrast, eSR genes tended to exhibit relatively greater pairwise haplotype sharing, lower promoter diversity, and fewer nonsynonymous polymorphisms, suggesting purifying selection or selective sweeps. Our results indicate that cis-regulatory evolution and genetic variation in stress responsive gene expression may be important mechanisms of local adaptation to climatic selective gradients.

  15. [Experiences with acute moderate controlled hemodilution (author's transl)].

    PubMed

    Orlowski, T; Lepert, L; Modrzewski, A

    1976-01-01

    The authors present an own method of hemodilution and experiences gained from 10 patients operated for chronic gastric or duodenal ulcer. The therapuetic results of the method show that it can be applied easely and safely in everyday surgical practice. In the patients treated with it no surgical and anaesthesiological complications could be observed during the operations as well as after them. The autors believe that the method of hemodilution for planned surgical operations will contribute to a more economical use of blood transfusions and to reduction in the occurrence of virus hepatitis.

  16. Optimal control theory--closing the gap between theory and experiment.

    PubMed

    von den Hoff, Philipp; Thallmair, Sebastian; Kowalewski, Markus; Siemering, Robert; de Vivie-Riedle, Regina

    2012-11-14

    Optimal control theory and optimal control experiments are state-of-the-art tools to control quantum systems. Both methods have been demonstrated successfully for numerous applications in molecular physics, chemistry and biology. Modulated light pulses could be realized, driving these various control processes. Next to the control efficiency, a key issue is the understanding of the control mechanism. An obvious way is to seek support from theory. However, the underlying search strategies in theory and experiment towards the optimal laser field differ. While the optimal control theory operates in the time domain, optimal control experiments optimize the laser fields in the frequency domain. This also implies that both search procedures experience a different bias and follow different pathways on the search landscape. In this perspective we review our recent developments in optimal control theory and their applications. Especially, we focus on approaches, which close the gap between theory and experiment. To this extent we followed two ways. One uses sophisticated optimization algorithms, which enhance the capabilities of optimal control experiments. The other is to extend and modify the optimal control theory formalism in order to mimic the experimental conditions.

  17. Controlled Experiment Replication in Evaluation of E-Learning System's Educational Influence

    ERIC Educational Resources Information Center

    Grubisic, Ani; Stankov, Slavomir; Rosic, Marko; Zitko, Branko

    2009-01-01

    We believe that every effectiveness evaluation should be replicated at least in order to verify the original results and to indicate evaluated e-learning system's advantages or disadvantages. This paper presents the methodology for conducting controlled experiment replication, as well as, results of a controlled experiment and an internal…

  18. Digital Fly-By-Wire Flight Control Validation Experience

    NASA Technical Reports Server (NTRS)

    Szalai, K. J.; Jarvis, C. R.; Krier, G. E.; Megna, V. A.; Brock, L. D.; Odonnell, R. N.

    1978-01-01

    The experience gained in digital fly-by-wire technology through a flight test program being conducted by the NASA Dryden Flight Research Center in an F-8C aircraft is described. The system requirements are outlined, along with the requirements for flight qualification. The system is described, including the hardware components, the aircraft installation, and the system operation. The flight qualification experience is emphasized. The qualification process included the theoretical validation of the basic design, laboratory testing of the hardware and software elements, systems level testing, and flight testing. The most productive testing was performed on an iron bird aircraft, which used the actual electronic and hydraulic hardware and a simulation of the F-8 characteristics to provide the flight environment. The iron bird was used for sensor and system redundancy management testing, failure modes and effects testing, and stress testing in many cases with the pilot in the loop. The flight test program confirmed the quality of the validation process by achieving 50 flights without a known undetected failure and with no false alarms.

  19. Citrus Quality Control: An NMR/MRI Problem-Based Experiment

    ERIC Educational Resources Information Center

    Erhart, Sarah E.; McCarrick, Robert M.; Lorigan, Gary A.; Yezierski, Ellen J.

    2016-01-01

    An experiment seated in an industrial context can provide an engaging framework and unique learning opportunity for an upper-division physical chemistry laboratory. An experiment that teaches NMR/MRI through a problem-based quality control of citrus products was developed. In this experiment, using a problem-based learning (PBL) approach, students…

  20. Involvement of a community in schistosomiasis control: a Kenyan experience.

    PubMed

    Katsivo, M N; Muthami, L N; Kimani, S; Karama, M; Kingori, F

    1993-08-01

    This paper illustrates how community participation was achieved in a study that was carried out between 1983-1988 whose general objective was to reduce the transmission of schistosomiasis in a rice irrigation scheme in Kenya. A community of 2,219 people was mobilized through health education, to effect changes in behaviour regarding water contact, water use and faecal disposal. Health education, alternative water sources, latrines and mass chemotherapy with praziquantel were the schistosomiasis control strategies utilized in this study. By the end of 2 years, the canals were cleared of their thick vegetation, water, sanitation, bathing and washing facilities were constructed, water contact with infective waters was reduced, faecal contamination of water was reduced, the people's knowledge on schistosomiasis increased and the rate and intensity of schistosomiasis infection was reduced. The study demonstrated that it is possible to fully involve the community in disease control efforts.

  1. Experience with joint AGC regulation. [Automatic Generation Control

    SciTech Connect

    Prowse, D.C.H. ); Koskela, P. ); Grove, T.A. ); Larson, L.R. )

    1994-11-01

    Operating generators on Automatic Generation Control to follow load fluctuations has often been considered an unavoidable cost of doing business. However, several months of testing and more than two years of commercial operation have demonstrated that there can be significant benefits in transferring AGC regulation from three predominantly thermal-based utilities to a fourth predominantly hydro-based utility. This arrangement called Joint AGC Regulation successfully transfers high frequency regulation components for reduced fuel and maintenance costs and allows dynamic interchange scheduling to achieve flexible generation scheduling and improved economic dispatch. Successful implementation involved development of a Joint AGC Regulation control strategy and an evaluation of the tangible and intangible costs of providing AGC regulation.

  2. Active spacecraft potential control: An ion emitter experiment. [Cluster mission

    NASA Technical Reports Server (NTRS)

    Riedler, W.; Goldstein, R.; Hamelin, M.; Maehlum, B. N.; Troim, J.; Olsen, R. C.; Pedersen, A.; Grard, R. J. L.; Schmidt, R.; Rudenauer, F.

    1988-01-01

    The cluster spacecraft are instrumented with ion emitters for charge neutralization. The emitters produce indium ions at 6 keV. The ion current is adjusted in a feedback loop with instruments measuring the spacecraft potential. The system is based on the evaporation of indium in the apex field of a needle. The design of the active spacecraft potential control instruments, and the ion emitters is presented.

  3. Active Thermal Control Experiments for LISA Ground Verification Testing

    NASA Astrophysics Data System (ADS)

    Higuchi, Sei; DeBra, Daniel B.

    2006-11-01

    The primary mission goal of LISA is detecting gravitational waves. LISA uses laser metrology to measure the distance between proof masses in three identical spacecrafts. The total acceleration disturbance to each proof mass is required to be below 3 × 10-15 m/s2√Hz . Optical path length variations on each optical bench must be kept below 40 pm/√Hz over 1 Hz to 0.1 mHz. Thermal variations due to, for example, solar radiation or temperature gradients across the proof mass housing will distort the spacecraft causing changes in the mass attraction and sensor location. We have developed a thermal control system developed for the LISA gravitational reference sensor (GRS) ground verification testing which provides thermal stability better than 1 mK/√Hz to f < 1 mHz and which by extension is suitable for in-flight thermal control for the LISA spacecraft to compensate solar irradiation. Thermally stable environment is very demanded for LISA performance verification. In a lab environment specifications can be met with considerable amount of insulation and thermal mass. For spacecraft, the very limited thermal mass calls for an active control system which can meet disturbance rejection and stability requirements simultaneously in the presence of long time delay. A simple proportional plus integral control law presently provides approximately 1 mK/√Hz of thermal stability for over 80 hours. Continuing development of a model predictive feed-forward algorithm will extend performance to below 1 mK/√Hz at f < 1 mHz and lower.

  4. Remote Manipulator System (RMS)-based Controls-Structures Interaction (CSI) flight experiment feasibility study

    NASA Technical Reports Server (NTRS)

    Demeo, Martha E.

    1990-01-01

    The feasibility of an experiment which will provide an on-orbit validation of Controls-Structures Interaction (CSI) technology, was investigated. The experiment will demonstrate the on-orbit characterization and flexible-body control of large flexible structure dynamics using the shuttle Remote Manipulator System (RMS) with an attached payload as a test article. By utilizing existing hardware as well as establishing integration, operation and safety algorithms, techniques and procedures, the experiment will minimize the costs and risks of implementing a flight experiment. The experiment will also offer spin-off enhancement to both the Shuttle RMS (SRMS) and the Space Station RMS (SSRMS).

  5. Optical and control modeling for adaptive beam-combining experiments

    SciTech Connect

    Gruetzner, J.K.; Tucker, S.D.; Neal, D.R.; Bentley, A.E.; Simmons-Potter, K.

    1995-08-01

    The development of modeling algorithms for adaptive optics systems is important for evaluating both performance and design parameters prior to system construction. Two of the most critical subsystems to be modeled are the binary optic design and the adaptive control system. Since these two are intimately related, it is beneficial to model them simultaneously. Optic modeling techniques have some significant limitations. Diffraction effects directly limit the utility of geometrical ray-tracing models, and transform techniques such as the fast fourier transform can be both cumbersome and memory intensive. The authors have developed a hybrid system incorporating elements of both ray-tracing and fourier transform techniques. In this paper they present an analytical model of wavefront propagation through a binary optic lens system developed and implemented at Sandia. This model is unique in that it solves the transfer function for each portion of a diffractive optic analytically. The overall performance is obtained by a linear superposition of each result. The model has been successfully used in the design of a wide range of binary optics, including an adaptive optic for a beam combining system consisting of an array of rectangular mirrors, each controllable in tip/tilt and piston. Wavefront sensing and the control models for a beam combining system have been integrated and used to predict overall systems performance. Applicability of the model for design purposes is demonstrated with several lens designs through a comparison of model predictions with actual adaptive optics results.

  6. Technology innovations and experience curves for nitrogen oxides control technologies.

    PubMed

    Yeh, Sonia; Rubin, Edward S; Taylor, Margaret R; Hounshell, David A

    2005-12-01

    This paper reviews the regulatory history for nitrogen oxides (NOx) pollutant emissions from stationary sources, primarily in coal-fired power plants. Nitrogen dioxide (NO2) is one of the six criteria pollutants regulated by the 1970 Clean Air Act where National Ambient Air Quality Standards were established to protect public health and welfare. We use patent data to show that in the cases of Japan, Germany, and the United States, innovations in NOx control technologies did not occur until stringent government regulations were in place, thus "forcing" innovation. We also demonstrate that reductions in the capital and operation and maintenance (O&M) costs of new generations of high-efficiency NOx control technologies, selective catalytic reduction (SCR), are consistently associated with the increasing adoption of the control technology: the so-called learning-by-doing phenomena. The results show that as cumulative world coal-fired SCR capacity doubles, capital costs decline to approximately 86% and O&M costs to 58% of their original values. The observed changes in SCR technology reflect the impact of technological advance as well as other factors, such as market competition and economies of scale.

  7. Fugitive dust control experiments using soil fixatives on vehicle traffic surfaces

    SciTech Connect

    Winberg, M.R.; Wixom, V.E.

    1992-08-01

    This report presents the results of engineering scale dust control experiments using soil fixative for contamination control during handling of transuranic waste. These experiments focused on controlling dust during retrieval operations of buried waste where waste and soil are intimately mixed. Sources of dust generation during retrieval operations include digging, dumping, and vehicle traffic. Because contaminants are expected to attach to soil particles and move with the generated dust, control of the dust spread may be the key to contamination control. Dust control techniques examined in these experiments include the use of soil fixatives to control generation of fugitive dusts during vehicle traffic operations. Previous experiments conducted in FY 1990 included testing of the soil fixative, ENTAC. These experiments showed that ENTAC was effective in controlling dust generation but had several undesirable properties such as slow cure times and clogged the pumps and application nozzles. Therefore, other products would have to be evaluated to find a suitable candidate. As a result, two soil fixatives were tested in these present experiments, COHEREX-PM, an asphalt emulsion product manufactured by Witco Corporation and FLAMBINDER, a calcium lignosulfonate product manufactured by Flambeau Corporation. The results of the experiments include product performance and recommended application methods for application in a field deployable contamination control unit to be built in FY 1993.

  8. Coherent operation of detector systems and their readout electronics in a complex experiment control environment

    NASA Astrophysics Data System (ADS)

    Koestner, Stefan

    2009-09-01

    With the increasing size and degree of complexity of today's experiments in high energy physics the required amount of work and complexity to integrate a complete subdetector into an experiment control system is often underestimated. We report here on the layered software structure and protocols used by the LHCb experiment to control its detectors and readout boards. The experiment control system of LHCb is based on the commercial SCADA system PVSS II. Readout boards which are outside the radiation area are accessed via embedded credit card sized PCs which are connected to a large local area network. The SPECS protocol is used for control of the front end electronics. Finite state machines are introduced to facilitate the control of a large number of electronic devices and to model the whole experiment at the level of an expert system.

  9. Recent Controlled Meteorological Balloon experiments in Queen Maud Land, Antarctica

    NASA Astrophysics Data System (ADS)

    Hole, L. R.; Voss, P. B.; Vihma, T. P.

    2013-12-01

    Controlled Meteorological (CMET) balloons are unique in that their altitude can be changed at any time during flight. They are remotely controlled via the Iridium network and use GPS for positioning. Over the past seven years, they have been operated at altitudes from sea-level to six kilometers and have flown for periods as long as five days. Campaigns have been carried out from the Amazon via Mexico City to polar regions. CMET balloons can perform repeated soundings in order to probe evolving thermal and chemical structure, measure wind shear, and track atmospheric layers. Typical ascent/descent rate is 1 m/s and the data sampling rate is 10 sec. The standard CMET balloon consists of zero-pressure balloon (~300-500 liters at sea level) which itself contains a much smaller (~100 liter) super-pressure balloon. Transferring helium between the super-pressure balloon and the zero-pressure balloon regulates the volume (and density) of the system, leading to controlled ascent and descent. Due to the rarity of meteorological observations from the Antarctic, especially from inland and over the sea, CMET balloons have potential to provide strongly needed data for evaluation of numerical weather prediction and climate models. Here, we present data from a CMET campaign carried out at the Finnish Aboa station in Antarctica (73° 03' S, 13° 25' W) in January 2013. The campaign was unique in that three CMET balloons were shipped to the station and launched by the local team. After the launch, they were controlled by scientists located in MA, USA and Norway. One balloon, Bravo, cruised for more than 100 hours over the coastal slopes of Queen Maud Land and nearby sea ice with a total trajectory length of over 3000 km (Fig. 1). It also passed nearby the UK Halley station. The altitude was generally kept at about 3000-3500 masl, but 8 controlled soundings down to 400-500 masl were carried out. The balloon data were compared with the Weather Research and Forecasting model (WRF

  10. Monitoring hyporheic exchanges during a dam controlled experiment

    NASA Astrophysics Data System (ADS)

    Houzé, Clémence; Varnède, Lucie; Durand, Véronique; Pessel, Marc

    2016-04-01

    Precise understanding of the hyporheic exchanges response to stream flow fluctuations remains a great challenge for many environmental and hydrological problems. Multiplication of natural stream restoration programs and anthropic structures removal highlight that a better understanding of the hydrodynamic and ecological functioning of hyporheic exchanges is critical . The objective of this field experiment was to monitor the dynamic exchanges within the hyporheic zone due to an artificial stream head variation. Various types of measurements were performed, using natural tracers and electrical resistivity tomography (ERT). The dam downstream the studied river reach was successively lowered during two days, and raised during three days, implying river heads variations of about 15cm. The studied area was equipped with CTD probes (measuring the head and the conductivity) within the river, 2 multi-depths water sampling tubes inserted up to one meter depth within the riverbed deposits and 3 ERT profiles with various electrode spacing (20 cm, 25 cm, 50 cm). During the 5 days experiment, water sampling and ERT profiles were done regularly. Estimations of the sediments hydraulic conductivity were obtained by several slug tests in plastic tubes at different depths within the streambed. First results showed that stream fluctuation leads to a rapid hyporheic response according to chloride variations between stream and riverbed sediments. Similar results between geochemical and geophysical tools were found. A decrease in stream head leads to reduce the depth of the mixing zone, as the river gaining conditions intensify. On the contrary, we observed that an increased river head tends to deepen the hyporheic exchange zone.

  11. The impact of abiotic factors on cellulose synthesis.

    PubMed

    Wang, Ting; McFarlane, Heather E; Persson, Staffan

    2016-01-01

    As sessile organisms, plants require mechanisms to sense and respond to changes in their environment, including both biotic and abiotic factors. One of the most common plant adaptations to environmental changes is differential regulation of growth, which results in growth either away from adverse conditions or towards more favorable conditions. As cell walls shape plant growth, this differential growth response must be accompanied by alterations to the plant cell wall. Here, we review the impact of four abiotic factors (osmotic conditions, ionic stress, light, and temperature) on the synthesis of cellulose, an important component of the plant cell wall. Understanding how different abiotic factors influence cellulose production and addressing key questions that remain in this field can provide crucial information to cope with the need for increased crop production under the mounting pressures of a growing world population and global climate change.

  12. Integrated metabolomics for abiotic stress responses in plants.

    PubMed

    Nakabayashi, Ryo; Saito, Kazuki

    2015-04-01

    Plants are considered to biosynthesize specialized (traditionally called secondary) metabolites to adapt to environmental stresses such as biotic and abiotic stresses. The majority of specialized metabolites induced by abiotic stress characteristically exhibit antioxidative activity in vitro, but their function in vivo is largely yet to be experimentally confirmed. In this review, we highlight recent advances in the identification of the role of abiotic stress-responsive specialized metabolites with an emphasis on flavonoids. Integrated 'omics' analysis, centered on metabolomics with a series of plant resources differing in their flavonoid accumulation, showed experimentally that flavonoids play a major role in antioxidation in vivo. In addition, the results also suggest the role of flavonoids in the vacuole. To obtain more in-depth insights, chemical and biological challenges need to be addressed for the identification of unknown specialized metabolites and their in vivo functions.

  13. Abiotic and biotic controls of spatial pattern at alpine treeline

    USGS Publications Warehouse

    Malanson, George P.; Xiao, Ningchuan; Alftine, K.J.; Bekker, Mathew; Butler, David R.; Brown, Daniel G.; Cairns, David M.; Fagre, Daniel; Walsh, Stephen J.

    2000-01-01

    At alpine treeline, trees and krummholz forms affect the environment in ways that increase their growth and reproduction. We assess the way in which these positive feedbacks combine in spatial patterns to alter the environment in the neighborhood of existing plants. The research is significant because areas of alpine tundra are susceptible to encroachment by woody species as climate changes. Moreover, understanding the general processes of plant invasion is important. The importance of spatial pattern has been recognized, but the spatial pattern of positive feedbacks per se has not been explored in depth. We present a linked set of models of vegetation change at an alpine forest-tundra ecotone. Our aim is to create models that are as simple as possible in order to test specific hypotheses. We present results from a model of the resource averaging hypothesis and the positive feedback switch hypothesis of treelines. We compare the patterns generated by the models to patterns observed in fine scale remotely sensed data.

  14. Japanese style total quality control: A first hand experience

    NASA Astrophysics Data System (ADS)

    Cook, S. C.

    1991-11-01

    The scope, concepts and practices of Total Quality Control (TQC) as operated in Japan today are described. The following mechanisms and elements are introduced: policy deployment, cross functional management, small group activities, suggestion systems, standardization, the TQC promotion department, and TQC education. It is shown on a practical level, how TQC operates in the research and engineering divisions of the aeroengine and space operation at Ishikawajima Harima Industries in Japan. Japanese style TQC is a well developed, well promoted, well structured, common approach to achieving customer satisfaction through continuous improvements. Furthermore, TQC itself is being continuously improved with the Japanese still refining, extending, and developing TQC.

  15. Recent BeO-reflector-controlled reactor experiments in ZPPR

    SciTech Connect

    McFarlane, H.F.; Brumbach, S.B.; Carpenter, S.G.; Collins, P.J.; McKnight, R.D.

    1986-01-01

    Integral reactor physics measurements were performed on a BeO-reflected fast reactor assembly in the ZPPR facility during January and February of 1985. The measurements emphasized power distributions and reflector control worths in two different critical states. The measurements have been analyzed using three-dimensional deterministic and Monte Carlo methods and the ENDF/B-V.2 nuclear data library. Together the measurements and analyses form a modern, reliable, benchmark data set for testing calculational methods that will be used in predicting some of the design parameters for future space reactors.

  16. Shuttle Orbiter Active Thermal Control Subsystem design and flight experience

    NASA Technical Reports Server (NTRS)

    Bond, Timothy A.; Metcalf, Jordan L.; Asuncion, Carmelo

    1991-01-01

    The paper examines the design of the Space Shuttle Orbiter Active Thermal Control Subsystem (ATCS) constructed for providing the vehicle and payload cooling during all phases of a mission and during ground turnaround operations. The operation of the Shuttle ATCS and some of the problems encountered during the first 39 flights of the Shuttle program are described, with special attention given to the major problems encountered with the degradation of the Freon flow rate on the Orbiter Columbia, the Flash Evaporator Subsystem mission anomalies which occurred on STS-26 and STS-34, and problems encountered with the Ammonia Boiler Subsystem. The causes and the resolutions of these problems are discussed.

  17. Automated Testing Experience of the Linear Aerospike SR-71 Experiment (LASRE) Controller

    NASA Technical Reports Server (NTRS)

    Larson, Richard R.

    1999-01-01

    System controllers must be fail-safe, low cost, flexible to software changes, able to output health and status words, and permit rapid retest qualification. The system controller designed and tested for the aerospike engine program was an attempt to meet these requirements. This paper describes (1) the aerospike controller design, (2) the automated simulation testing techniques, and (3) the real time monitoring data visualization structure. Controller cost was minimized by design of a single-string system that used an off-the-shelf 486 central processing unit (CPU). A linked-list architecture, with states (nodes) defined in a user-friendly state table, accomplished software changes to the controller. Proven to be fail-safe, this system reported the abort cause and automatically reverted to a safe condition for any first failure. A real time simulation and test system automated the software checkout and retest requirements. A program requirement to decode all abort causes in real time during all ground and flight tests assured the safety of flight decisions and the proper execution of mission rules. The design also included health and status words, and provided a real time analysis interpretation for all health and status data.

  18. Next generation optical instruments and space experiments based on the LDEF thermal control surfaces experiment (S0069)

    NASA Technical Reports Server (NTRS)

    Wilkes, Donald R.

    1992-01-01

    The Thermal Control Surfaces Experiment (TCSE) on the Long Duration Exposure Facility (LDEF) was a very effective experiment to study the effects of the space environment on thermal control surfaces. Three current programs have benefitted from the TCSE experiment and are the: Optical Properties Monitor (OPM); Laboratory Portable Spectroreflectometer (LPSR); and Space Portable Spectroreflectometer. The OPM expands the TCSE optical measurement and environmental monitoring capability. The LPSR is a hand held instrument developed to measure the spectral total hemispherical reflectance of small and larger surfaces in the field. The SPSR is a space application of the LPSR and TCSE technologies with the same measurement capabilities. The SPSR will be able to characterize the external surfaces of operational spacecraft such as Space Station Freedom.

  19. Control-based continuation: Bifurcation and stability analysis for physical experiments

    NASA Astrophysics Data System (ADS)

    Barton, David A. W.

    2017-02-01

    Control-based continuation is technique for tracking the solutions and bifurcations of nonlinear experiments. The idea is to apply the method of numerical continuation to a feedback-controlled physical experiment such that the control becomes non-invasive. Since in an experiment it is not (generally) possible to set the state of the system directly, the control target becomes a proxy for the state. Control-based continuation enables the systematic investigation of the bifurcation structure of a physical system, much like if it was numerical model. However, stability information (and hence bifurcation detection and classification) is not readily available due to the presence of stabilising feedback control. This paper uses a periodic auto-regressive model with exogenous inputs (ARX) to approximate the time-varying linearisation of the experiment around a particular periodic orbit, thus providing the missing stability information. This method is demonstrated using a physical nonlinear tuned mass damper.

  20. Strategies to ameliorate abiotic stress-induced plant senescence.

    PubMed

    Gepstein, Shimon; Glick, Bernard R

    2013-08-01

    The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield due to leaf yellowing and deteriorated photosynthesis, and is also responsible for the losses resulting from the short shelf life of many vegetables and fruits. Under abiotic stress conditions the yield losses are often even greater. The primary challenge in agricultural sciences today is to develop technologies that will increase food production and sustainability of agriculture especially under environmentally limiting conditions. In this chapter, some of the mechanisms involved in abiotic stress-induced plant senescence are discussed. Recent studies have shown that crop yield and nutritional values can be altered as well as plant stress tolerance through manipulating the timing of senescence. It is often difficult to separate the effects of age-dependent senescence from stress-induced senescence since both share many biochemical processes and ultimately result in plant death. The focus of this review is on abiotic stress-induced senescence. Here, a number of the major approaches that have been developed to ameliorate some of the effects of abiotic stress-induced plant senescence are considered and discussed. Some approaches mimic the mechanisms already used by some plants and soil bacteria whereas others are based on development of new improved transgenic plants. While there may not be one simple strategy that can effectively decrease all losses of crop yield that accrue as a consequence of abiotic stress-induced plant senescence, some of the strategies that are discussed already show great promise.

  1. The combined effects of biotic and abiotic stress on species richness and connectance.

    PubMed

    Kulkarni, Devdutt; De Laender, Frederik

    2017-01-01

    Food web structure and species richness are both subject to biotic (e.g. predation pressure and resource limitation) and abiotic stress (e.g. environmental change). We investigated the combined effects of both types of stress on richness and connectance, and on their relationship, in a predator-prey system. To this end, we developed a mathematical two trophic level food-web model to investigate the effects of biotic and abiotic stress on food web connectance and species richness. We found negative effects of top-down and bottom-up control on prey and predator richness, respectively. Effects of top-down and bottom-up control were stronger when initial connectance was high and low, respectively. Bottom-up control could either aggravate or buffer negative effects of top-down control. Abiotic stress affecting predator richness had positive indirect effects on prey richness, but only when initial connectance was low. However, no indirect effects on predator richness were observed following direct effects on prey richness. Top-down and bottom-up control selected for weakly connected prey and highly connected predators, thereby decreasing and increasing connectance, respectively. Our simulations suggest a broad range of negative and positive richness-connectance relationships, thereby revisiting the often found negative relationship between richness and connectance in food webs. Our results suggest that (1) initial food-web connectance strongly influences the effects of biotic stress on richness and the occurrence of indirect effects on richness; and (2) the shape of the richness-connectance relationship depends on the type of biotic stress.

  2. Elasticity improves handgrip performance and user experience during visuomotor control

    PubMed Central

    Rinne, Paul; Liardon, Jean-Luc; Uhomoibhi, Catherine; Bentley, Paul; Burdet, Etienne

    2017-01-01

    Passive rehabilitation devices, providing motivation and feedback, potentially offer an automated and low-cost therapy method, and can be used as simple human–machine interfaces. Here, we ask whether there is any advantage for a hand-training device to be elastic, as opposed to rigid, in terms of performance and preference. To address this question, we have developed a highly sensitive and portable digital handgrip, promoting independent and repetitive rehabilitation of grasp function based around a novel elastic force and position sensing structure. A usability study was performed on 66 healthy subjects to assess the effect of elastic versus rigid handgrip control during various visuomotor tracking tasks. The results indicate that, for tasks relying either on feedforward or on feedback control, novice users perform significantly better with the elastic handgrip, compared with the rigid equivalent (11% relative improvement, 9–14% mean range; p < 0.01). Furthermore, there was a threefold increase in the number of subjects who preferred elastic compared with rigid handgrip interaction. Our results suggest that device compliance is an important design consideration for grip training devices. PMID:28386448

  3. Fluorescence measurements of the thermal control experiments coatings on LDEF S0069 and A0114

    NASA Technical Reports Server (NTRS)

    Zwiener, J. M.; Mell, R. J.; Peters, P. N.; Gregory, J. C.; Wilkes, D. R.; Miller, E. R.

    1993-01-01

    Fluorescence measurements were made on the thermal control coatings from the Long Duration Experiment Facility (LDEF) S0069, Thermal Control Surfaces Experiment (TCSE); and the A0114, Interaction of Atomic Oxygen with Material Surfaces in Low Earth orbit. Fluorescence was observed in two types of thermal control coatings and is attributed to pigments or binders. In addition, fluorescence measurement on the silver Teflon from the front cover of TCSE led to confirmation of damage (cracking) to the metal layers during application.

  4. [Spreading experience of demonstration plots and strengthening control of parasitic diseases].

    PubMed

    Yu, Wang

    2011-10-01

    This paper summarizes the achievements and experiences of demonstration plots carrying out comprehensive control measures of parasite diseases in China, and elaborates the hard task of the control of parasitic diseases as one of main public health problems. The article also elaborates the significance of spreading the experiences of demonstration plots carrying out comprehensive control measures of parasite diseases for improving the health of people and promoting the construction of new countryside.

  5. A proposed abiotic reaction scheme for hydroxylamine and monochloramine under chloramination relevant drinking water conditions.

    PubMed

    Wahman, David G; Speitel, Gerald E; Machavaram, Madhav V

    2014-09-01

    Drinking water monochloramine (NH2Cl) use may promote ammonia-oxidizing bacteria (AOB). AOB use (i) ammonia monooxygenase for biological ammonia (NH3) oxidation to hydroxylamine (NH2OH) and (ii) hydroxylamine oxidoreductase for NH2OH oxidation to nitrite. NH2Cl and NH2OH may react, providing AOB potential benefits and detriments. The NH2Cl/NH2OH reaction would benefit AOB by removing the disinfectant (NH2Cl) and releasing their growth substrate (NH3), but the NH2Cl/NH2OH reaction would also provide a possible additional inactivation mechanism besides direct NH2Cl reaction with cells. Because biological NH2OH oxidation supplies the electrons required for biological NH3 oxidation, the NH2Cl/NH2OH reaction provides a direct mechanism for NH2Cl to inhibit NH3 oxidation, starving the cell of reductant by preventing biological NH2OH oxidation. To investigate possible NH2Cl/NH2OH reaction implications on AOB, an understanding of the underlying abiotic reaction is first required. The present study conducted a detailed literature review and proposed an abiotic NH2Cl/NH2OH reaction scheme (RS) for chloramination relevant drinking water conditions (μM concentrations, air saturation, and pH 7-9). Next, RS literature based kinetics and end-products were evaluated experimentally between pHs 7.7 and 8.3, representing (i) the pH range for future experiments with AOB and (ii) mid-range pHs typically found in chloraminated drinking water. In addition, a (15)N stable isotope experiment was conducted to verify nitrous oxide and nitrogen gas production and their nitrogen source. Finally, the RS was slightly refined using the experimental data and an AQUASIM implemented kinetic model. A chloraminated drinking water relevant RS is proposed and provides the abiotic reaction foundation for future AOB biotic experiments.

  6. Nitric oxide signaling in plant responses to abiotic stresses.

    PubMed

    Qiao, Weihua; Fan, Liu-Min

    2008-10-01

    Nitric oxide (NO) plays important roles in diverse physiological processes in plants. NO can provoke both beneficial and harmful effects, which depend on the concentration and location of NO in plant cells. This review is focused on NO synthesis and the functions of NO in plant responses to abiotic environmental stresses. Abiotic stresses mostly induce NO production in plants. NO alleviates the harmfulness of reactive oxygen species, and reacts with other target molecules, and regulates the expression of stress responsive genes under various stress conditions.

  7. Starch as a determinant of plant fitness under abiotic stress.

    PubMed

    Thalmann, Matthias; Santelia, Diana

    2017-03-09

    I. II. III. IV. V. VI. References SUMMARY: Abiotic stresses, such as drought, high salinity and extreme temperatures, pose one of the most important constraints to plant growth and productivity in many regions of the world. A number of investigations have shown that plants, including several important crops, remobilize their starch reserve to release energy, sugars and derived metabolites to help mitigate the stress. This is an essential process for plant fitness with important implications for plant productivity under challenging environmental conditions. In this Tansley insight, we evaluate the current literature on starch metabolism in response to abiotic stresses, and discuss the key enzymes involved and how they are regulated.

  8. Laboratory Experiments on the Control of Cyclops Transmitting Guinea Worm

    PubMed Central

    Muller, Ralph

    1970-01-01

    Infection with the guinea worm (Dracunculus medinensis) affects millions of people living in rural areas in certain parts of the world. Usually, the standard of living is low and elaborate control measures would not be feasible. In this study, various insecticidal and molluscicidal compounds were tested in the laboratory for their toxicity to the intermediate hosts, namely, various species of cyclops, which often live in sources of potable water, such as step-wells, cisterns and ponds. Cheapness, low toxicity to mammals and ease of application, in conjunction with effectiveness against cyclops, are primary requisites for any compound accepted for widespread use. Abate, an insecticide, has been shown to be particularly promising in these respects. The timing and mode of application of compounds toxic to cyclops in various endemic regions are discussed. PMID:4247122

  9. Remote Control and Monitoring of VLBI Experiments by Smartphones

    NASA Astrophysics Data System (ADS)

    Ruztort, C. H.; Hase, H.; Zapata, O.; Pedreros, F.

    2012-12-01

    For the remote control and monitoring of VLBI operations, we developed a software optimized for smartphones. This is a new tool based on a client-server architecture with a Web interface optimized for smartphone screens and cellphone networks. The server uses variables of the Field System and its station specific parameters stored in the shared memory. The client running on the smartphone by a Web interface analyzes and visualizes the current status of the radio telescope, receiver, schedule, and recorder. In addition, it allows commands to be sent remotely to the Field System computer and displays the log entries. The user has full access to the entire operation process, which is important in emergency cases. The software also integrates a webcam interface.

  10. First Experiences Using XACML for Access Control in Distributed Systems

    NASA Technical Reports Server (NTRS)

    Lorch, Marcus; Proctor, Seth; Lepro, Rebekah; Kafura, Dennis; Shah, Sumit

    2003-01-01

    Authorization systems today are increasingly complex. They span domains of administration, rely on many different authentication sources, and manage permissions that can be as complex as the system itself. Worse still, while there are many standards that define authentication mechanisms, the standards that address authorization are less well defined and tend to work only within homogeneous systems. This paper presents XACML, a standard access control language, as one component of a distributed and inter-operable authorization framework. Several emerging systems which incorporate XACML are discussed. These discussions illustrate how authorization can be deployed in distributed, decentralized systems. Finally, some new and future topics are presented to show where this work is heading and how it will help connect the general components of an authorization system.

  11. Radiative Transfer Theory Verified by Controlled Laboratory Experiments

    NASA Technical Reports Server (NTRS)

    Mishchenko, Michael I.; Goldstein, Dennis H.; Chowdhary, Jacek; Lompado, Arthur

    2013-01-01

    We report the results of high-accuracy controlled laboratory measurements of the Stokes reflection matrix for suspensions of submicrometer-sized latex particles in water and compare them with the results of a numerically exact computer solution of the vector radiative transfer equation (VRTE). The quantitative performance of the VRTE is monitored by increasing the volume packing density of the latex particles from 2 to 10. Our results indicate that the VRTE can be applied safely to random particulate media with packing densities up to 2. VRTE results for packing densities of the order of 5 should be taken with caution, whereas the polarized bidirectional reflectivity of suspensions with larger packing densities cannot be accurately predicted. We demonstrate that a simple modification of the phase matrix entering the VRTE based on the so-called static structure factor can be a promising remedy that deserves further examination.

  12. Control of malaria: a successful experience from Viet Nam.

    PubMed Central

    Hung, Le Q.; Vries, Peter J. de; Giao, Phan T.; Nam, Nguyen V.; Binh, Tran Q.; Chong, M. T.; Quoc, N. T. T. A.; Thanh, T. N.; Hung, L. N.; Kager, P. A.

    2002-01-01

    OBJECTIVE: To follow malaria prospectively in an ethnic minority commune in the south of Viet Nam with high malaria transmission and seasonal fluctuation, during malaria control interventions using insecticide-treated bednets (ITBNs) and early diagnosis and treatment (EDT) of symptomatic patients. METHODS: From 1994 onwards the following interventions were used: distribution of ITBNs to all households with biannual reimpregnation; construction of a health post and appointment of staff trained in microscopic diagnosis and treatment of malaria; regular supply of materials and drugs; annual cross-sectional malaria surveys with treatment of all parasitaemic subjects, and a programme of community involvement and health education. Surveys were held yearly at the end of the rainy season. During the surveys, demographic data were updated. Diagnosis and treatment of malaria were free of charge. Plasmodium falciparum infection was treated with artesunate and P. vivax infection with chloroquine plus primaquine. FINDINGS: The baseline survey in 1994 recorded 716 inhabitants. Of the children under 2 years of age, 37% were parasitaemic; 56% of children aged 2-10 years, and 35% of the remaining population were parasitaemic. P. falciparum accounted for 73-79% of these infections. The respective splenomegaly rates for the above-mentioned age groups were 20%, 56%, and 32%. In 1999, the proportion of parasitaemic subjects was 4%, 7% and 1%, respectively, of which P.falciparum contributed 56%. The splenomegaly rate was 0%, 5% and 2%, respectively. CONCLUSIONS: A combination of ITBNs and EDT, provided free of charge, complemented by annual diagnosis and treatment during malaria surveys and community involvement with health education successfully brought malaria under control. This approach could be applied to other regions in the south of Viet Nam and provides a sound basis for further studies in other areas with different epidemiological patterns of malaria. PMID:12219158

  13. Abiotic Dissolved Organic Matter-Mineral Interaction in the Karstic Floridan Aquifer

    NASA Astrophysics Data System (ADS)

    Jin, J.; Zimmerman, A.

    2007-12-01

    Dissolved organic matter (DOM)-mineral interaction (e.g. adsorption, desorption, mineral dissolution) in groundwater is a significant factor controlling geochemical, environmental and microbial processes and may be helpful in efforts to track groundwater sources or contaminant fate. Despite its importance, the dynamics and consequences of these abiotic interactions remain poorly understood, largely due to the inaccessibility and heterogeneity of the subsurface, as well as the chemical complexity of DOM. This study models the OM-mineral interactions that takes place in the Floridan aquifer through laboratory adsorption-desorption experiments using DOM (groundwater, river water, soil extracts) and carbonate minerals (calcite, dolomite) collected in north Florida. High performance liquid chromatography-size exclusion chromatography (HPLC-SEC) and UV-fluorescence excitation-emission matrix (EEM) spectrophotometry was used to examine the organic compound types exhibiting preferential affinity for carbonate minerals. Our results show that the DOM-carbonate adsorption/desorption isotherms are well described by the Freundlich model. Freundlich exponents (average value: 0.6488) less than one indicated a filling of adsorption sites. Minerals from Ocala tend to have higher adsorption affinity as well as adsorption capacity than those from Suwannee River Basin; however, both were found to have mineral dissolution. Two fluorescent signals, indicative of a fulvic-like (at excitation wavelength 295-310 nm, emission 400-420 nm) and a protein-like (275/345nm) moiety, were detected in DOM. A reduction in the fulvic-like peak intensity occurred following carbonate adsorption while the protein-like peaks remain almost unchanged indicating the preferential adsorption of fulvic acids. HPLC-SEC results (DOM properties as a function of molecular weight) will be discussed. The chemical properties of DOM in environmental groundwater samples will also be presented and evaluated in light of

  14. [Management control of cardiology: the experience of a departmental unit].

    PubMed

    Boccanelli, A; Spandonaro, F

    2000-01-01

    In most Italian hospitals, sanitary reform is being applied, while at the same time a new organization of the National Health System is being planned. The director of the medical hospital (head doctor) is becoming more and more involved in management and this aspect has modified his professional attributes. Cardiology is a branch of medicine that, through its scientific preparatory work consisting in debates, management courses, ethics, and production of managerial software, is closer to applying the reform without risking improper administrative aspects. This, obviously, comes about after thoroughly reviewing past work methods and the need to have an administrative organization, which allocates efficient use of manpower and materials, helping to eliminate any sources of inefficiency. The logical procedure foresees an actual analysis in terms of sanitary needs and availability of resources, and so attempting to better balance and harmonize both aspects of the problem. Certainly, the acquisition of theoretical norms and practices, which today are present because of the upsurge in training courses for doctors, is not enough to guarantee the achievement of optimal results. Furthermore, we find that theoretical models need to be validated and adapted to real work situations in the public hospital sector. This paper proposes, therefore, to explain the managerial experiences achieved in actual work situations at the Cardiology Department Unit of the San Giovanni Addolorata Hospital in Rome. In particular, it shows that in order to reach its clinical and economical objectives, it is essential to make available correct informative support for strategic and operational decisions. We can observe that there is a continuing lack of computer support systems being integrated into the present organization of most cardiology units. The use of software distributed to cardiology units from the Associazione Nazionale Medici Cardiologi Ospedalieri (ANMCO) has enabled us to partially

  15. Guidelines to use tomato in experiments with a controlled environment

    PubMed Central

    Schwarz, Dietmar; Thompson, Andrew J.; Kläring, Hans-Peter

    2014-01-01

    Domesticated tomato (Solanum lycopersicum) is the most important horticultural crop worldwide. Low polymorphism at the DNA level conflicts with the wealth of morphological variation. Fruits vary widely in size, shape, and color. In contrast, genetic variation between the 16 wild relatives is tremendous. Several large seed banks provide tomato germplasm for both domesticated and wild accessions of tomato. Recently, the genomes of the inbred cultivar “Heinz 1706” (≈900 Mb), and S. pimpinellifolium (739 Mb) were sequenced. Genomic markers and genome re-sequencing data are available for >150 cultivars and accessions. Transformation of tomato is relatively easy and T-DNA insertion line collections are available. Tomato is widely used as a model crop for fruit development but also for diverse physiological, cellular, biochemical, molecular, and genetic studies. It can be easily grown in greenhouses or growth chambers. Plants grow, flower, and develop fruits well at daily light lengths between 8 and 16 h. The required daily light integral of an experiment depends on growth stage and temperature investigated. Temperature must be 10–35°C, relative humidity 30–90%, and, CO2 concentration 200–1500 μmol mol−1. Temperature determines the speed of the phenological development while daily light integral and CO2 concentration affect photosynthesis and biomass production. Seed to seed cultivation takes 100 days at 20°C and can be shortened or delayed by temperature. Tomato may be cultivated in soil, substrates, or aeroponically without any substrate. Root volume, and water uptake requirements are primarily determined by transpiration demands of the plants. Many nutrient supply recipes and strategies are available to ensure sufficient supply as well as specific nutrient deficits/surplus. Using appropriate cultivation techniques makes tomato a convenient model plant for researchers, even for beginners. PMID:25477888

  16. A controlled field experiment on groundwater contamination by a multicomponent DNAPL: creation of the emplaced-source and overview of dissolved plume development.

    PubMed

    Rivett, M O; Feenstra, S; Cherry, J A

    2001-05-01

    A unique field experiment has been undertaken at the CFB Borden research site to investigate the development of dissolved chlorinated solvent plumes from a residual dense non-aqueous phase liquid (DNAPL) source. The "emplaced-source" tracer test methodology involved a controlled emplacement of a block-shaped source of sand containing chlorinated solvents below the water table. The gradual dissolution of this residual DNAPL solvent source under natural aquifer conditions caused dissolved solvent plumes of trichloromethane (TCM), trichloroethene (TCE) and perchloroethene (PCE) to continuously develop down gradient. Source dissolution and 3-D plume development were successfully monitored via 173 multilevel samplers over a 475-day tracer test period prior to site remediation research being initiated. Detailed groundwater level and hydraulic conductivity data were collected. Development of plumes with concentrations spanning 1-700,000 micrograms/1 is described and key processes controlling their migration identified. Plumes were observed to be narrow due to the weakness of transverse dispersion processes and long due to advection and significant longitudinal dispersion, very limited sorptive retardation and negligible, if any, attenuation due to biodegradation or abiotic reaction. TCM was shown to be essentially conservative, TCE very nearly conservative and PCE, consistent with its greater hydrophobicity, more retarded yet having a greater mobility than observed in previous Borden field tests. The absence of biodegradation was ascribed to the prevailing aerobic conditions and lack of any additional biodegradable carbon substrates. The transient groundwater flow regime caused significant transverse lateral plume movement, plume asymmetry and was likely responsible for most of the, albeit limited, transverse horizontal plume spreading. In agreement with the widespread incidence of extensive TCE and PCE plumes throughout the industrialized world, the experiment indicates

  17. Reducing sample size in experiments with animals: historical controls and related strategies.

    PubMed

    Kramer, Matthew; Font, Enrique

    2017-02-01

    Reducing the number of animal subjects used in biomedical experiments is desirable for ethical and practical reasons. Previous reviews of the benefits of reducing sample sizes have focused on improving experimental designs and methods of statistical analysis, but reducing the size of control groups has been considered rarely. We discuss how the number of current control animals can be reduced, without loss of statistical power, by incorporating information from historical controls, i.e. subjects used as controls in similar previous experiments. Using example data from published reports, we describe how to incorporate information from historical controls under a range of assumptions that might be made in biomedical experiments. Assuming more similarities between historical and current controls yields higher savings and allows the use of smaller current control groups. We conducted simulations, based on typical designs and sample sizes, to quantify how different assumptions about historical controls affect the power of statistical tests. We show that, under our simulation conditions, the number of current control subjects can be reduced by more than half by including historical controls in the analyses. In other experimental scenarios, control groups may be unnecessary. Paying attention to both the function and to the statistical requirements of control groups would result in reducing the total number of animals used in experiments, saving time, effort and money, and bringing research with animals within ethically acceptable bounds.

  18. The Navstar GPS master control station's Kalman filter experience

    NASA Technical Reports Server (NTRS)

    Scardera, Michael P.

    1990-01-01

    The Navstar Global Positioning System (GPS) is a highly accurate space based navigation system providing all weather, 24 hour a day service to both military and civilian users. The system provides a Gaussian position solution with four satellites, each providing its ephemeris and clock offset with respect to GPS time. The GPS Master Clock Station (MCS) is charged with tracking each Navstar spacecraft and precisely defining the ephemeris and clock parameters for upload into the vehicle's navigation message. Briefly described here are the Navstar system and the Kalman filter estimation process used by MCS to determine, predict, and ensure quality control for each of the satellite's ephemeris and clock states. Routine performance is shown. Kalman filter reaction and response is discussed for anomalous clock behavior and trajectory perturbations. Particular attention is given to MCS efforts to improve orbital adjust modeling. The satellite out of service time due to orbital maneuvering has been reduced in the past year from four days to under twelve hours. The planning, reference trajectory model, and Kalman filter management improvements are explained.

  19. Combined Effects of Soil Biotic and Abiotic Factors, Influenced by Sewage Sludge Incorporation, on the Incidence of Corn Stalk Rot

    PubMed Central

    Fortes, Nara Lúcia Perondi; Navas-Cortés, Juan A; Silva, Carlos Alberto; Bettiol, Wagner

    2016-01-01

    The objectives of this study were to evaluate the combined effects of soil biotic and abiotic factors on the incidence of Fusarium corn stalk rot, during four annual incorporations of two types of sewage sludge into soil in a 5-years field assay under tropical conditions and to predict the effects of these variables on the disease. For each type of sewage sludge, the following treatments were included: control with mineral fertilization recommended for corn; control without fertilization; sewage sludge based on the nitrogen concentration that provided the same amount of nitrogen as in the mineral fertilizer treatment; and sewage sludge that provided two, four and eight times the nitrogen concentration recommended for corn. Increasing dosages of both types of sewage sludge incorporated into soil resulted in increased corn stalk rot incidence, being negatively correlated with corn yield. A global analysis highlighted the effect of the year of the experiment, followed by the sewage sludge dosages. The type of sewage sludge did not affect the disease incidence. A multiple logistic model using a stepwise procedure was fitted based on the selection of a model that included the three explanatory parameters for disease incidence: electrical conductivity, magnesium and Fusarium population. In the selected model, the probability of higher disease incidence increased with an increase of these three explanatory parameters. When the explanatory parameters were compared, electrical conductivity presented a dominant effect and was the main variable to predict the probability distribution curves of Fusarium corn stalk rot, after sewage sludge application into the soil. PMID:27176597

  20. Combined Effects of Soil Biotic and Abiotic Factors, Influenced by Sewage Sludge Incorporation, on the Incidence of Corn Stalk Rot.

    PubMed

    Ghini, Raquel; Fortes, Nara Lúcia Perondi; Navas-Cortés, Juan A; Silva, Carlos Alberto; Bettiol, Wagner

    2016-01-01

    The objectives of this study were to evaluate the combined effects of soil biotic and abiotic factors on the incidence of Fusarium corn stalk rot, during four annual incorporations of two types of sewage sludge into soil in a 5-years field assay under tropical conditions and to predict the effects of these variables on the disease. For each type of sewage sludge, the following treatments were included: control with mineral fertilization recommended for corn; control without fertilization; sewage sludge based on the nitrogen concentration that provided the same amount of nitrogen as in the mineral fertilizer treatment; and sewage sludge that provided two, four and eight times the nitrogen concentration recommended for corn. Increasing dosages of both types of sewage sludge incorporated into soil resulted in increased corn stalk rot incidence, being negatively correlated with corn yield. A global analysis highlighted the effect of the year of the experiment, followed by the sewage sludge dosages. The type of sewage sludge did not affect the disease incidence. A multiple logistic model using a stepwise procedure was fitted based on the selection of a model that included the three explanatory parameters for disease incidence: electrical conductivity, magnesium and Fusarium population. In the selected model, the probability of higher disease incidence increased with an increase of these three explanatory parameters. When the explanatory parameters were compared, electrical conductivity presented a dominant effect and was the main variable to predict the probability distribution curves of Fusarium corn stalk rot, after sewage sludge application into the soil.

  1. Scalability of Robotic Controllers: An Evaluation of Controller Options-Experiment II

    DTIC Science & Technology

    2011-09-01

    screen controller was compared with their performance using a baseline Xbox 360 joystick controller. Thirty Soldiers from the Officers’ Candidate...Course completion times were significantly faster with the Xbox controller compared to the Android controller. In addition, there were significantly...fewer driving errors and off-course errors with the Xbox controller. Total workload ratings were significantly lower for the Xbox than for the Android

  2. Numerical controlled polishing, continued force wear and part correction experiments

    SciTech Connect

    Hannah, P.R.; Day, R.D.; Hatch, D.J.; McClure, E.R.

    1994-09-01

    This abstract reports the near completion of the first phase of this program. It is the aim of this program to provide the operator of a N/C diamond turning machine or N/C grinding machine (jig grinder) with the wear characteristics necessary to achieve uniform material removal. The second phase of this program addresses a different problem, although solving this problem is highly dependent on the results of the first phase. Diamond turned, or any lathe turned surface, exhibits regular tool marks due to the tool passing over the surface being cut. Changes in depth of cut, feed rate and work rpm will change the character of these groves, but will not eliminate them. Optical surfaces produced by this process exhibit increased scattering as the light wavelength decreases limiting their use; at least for optical purposes, to IR and some visible applications. Utilizing wear information gathered in the first part of this program we will attempt to reduce these residual tool marks by polishing. The polishing of diamond turned surfaces is not new. Diamond turned metal surfaces, especially in electroless nickel and high phosphorus nickel electroplate have been polished to improve their scatter characteristics. What we believe is unique is the use of a spherical wheel, rotating on axis and being moved over the part in a prescribed manner by numerical control. Over the past year we have made some major changes in our polishing methods and procedures. We have listed below these changes, as a refresher for the reader as to our previous procedures. These changes will be addressed in the body of the text.

  3. The Aqua-Planet Experiment (APE): CONTROL SST Simulation

    NASA Technical Reports Server (NTRS)

    Blackburn, Michael; Williamson, David L.; Nakajima, Kensuke; Ohfuchi, Wataru; Takahashi, Yoshiyuki O.; Hayashi, Yoshi-Yuki; Nakamura, Hisashi; Ishiwatari, Masaki; Mcgregor, John L.; Borth, Hartmut; Wirth, Volkmar; Frank, Helmut; Bechtold, Peter; Wedi, Nils P.; Tomita, Hirofumi; Satoh, Masaki; Zhao, Ming; Held, Isaac M.; Suarez, Max J.; Lee, Myong-In; Watanabe, Masahiro; Kimoto, Masahide; Liu, Yimin; Wang, Zaizhi; Molod, Andrea M.; Rajendran, Kavirajan; Kotoh, Akio; Stratton, Rachel

    2013-01-01

    experimental hierarchy used to evaluate AGCMs. This comparison does suggest that the range of model behaviour could be better understood and reduced in conjunction with Earth climate simulations. Controlled experimentation is required to explore individual model behavior and investigate convergence of the aqua-planet climate with increasing resolution.

  4. Genetic mapping of abiotic stress responses in sorghum

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Due to rich genetic diversity for tolerance to various abiotic stress conditions, sorghum is an ideal system for genetic mapping and elucidation of genome regions that confer such response among cereal crops. Coupled with the development of DNA marker technologies and most recently the sequencing o...

  5. ABIOTIC DEGRADATION OF TRICHLOROETHYLENE UNDER THERMAL REMEDIATION CONDITIONS

    EPA Science Inventory

    The degradation of TCE (C2HCl3) to carbon dioxide (CO2) and chloride (Cl-) has been reported to occur during thermal remediation of subsurface environments. The overall goal of this study was to evaluate abiotic degradation of TCE at el...

  6. Overview of CFD Validation Experiments for Circulation Control Applications at NASA

    NASA Technical Reports Server (NTRS)

    Jones, G. S.; Lin, J. C.; Allan, B. G.; Milholen, W. E.; Rumsey, C. L.; Swanson, R. C.

    2008-01-01

    Circulation control is a viable active flow control approach that can be used to meet the NASA Subsonic Fixed Wing project s Cruise Efficient Short Take Off and Landing goals. Currently, circulation control systems are primarily designed using empirical methods. However, large uncertainty in our ability to predict circulation control performance has led to the development of advanced CFD methods. This paper provides an overview of a systematic approach to developing CFD tools for basic and advanced circulation control applications. This four-step approach includes "Unit", "Benchmar", "Subsystem", and "Complete System" experiments. The paper emphasizes the ongoing and planned 2-D and 3-D physics orientated experiments with corresponding CFD efforts. Sample data are used to highlight the challenges involved in conducting circulation control computations and experiments.

  7. Canopy Level Solar Induced Fluorescence for Vegetation in Controlled Experiments

    NASA Technical Reports Server (NTRS)

    Middleton, E. M.; Corp, L. A.; Campbell, P. K. Entcheva

    2007-01-01

    Solar induced chlorophyll fluorescence (SIF) was retrieved from high resolution reflectance spectra acquired one meter above saplings of three deciduous tree species during springtime (three weeks after leaf flush) and in late summer when foliage was mature. SIF was determined by application of the Fraunhofer Line Depth (FLD) Principal to above-canopy spectra acquired with an Analytical Spectral Devices (ASD) Fieldspec spectroradiometer (3.2 nm resolution with 1.2 nm sampling interval). SIF retrievals were made at the two atmospheric oxygen (O2) absorption features that occur in the chlorophyll fluorescence (ChlF) region (660 -780 nm). These telluric features are 02V, the broader and deeper feature centered at 760 nm, but located on the shoulder of the far-red ChlF peak at 740 nm; and 023, a narrow feature centered at 688 nm that is positioned near the red ChlF peak at 685 nm. Supporting, coincident leaf level fluorescence, reflectance, photochemical and other measurements were also made. At the leaf level, these measurements included in situ photosynthetic capacity (Pmax) and light adapted total chlorophyll fluorescence (Fs') collected at steady state under high light and controlled chamber conditions (e.g., temperature, PAR, humidity, and COz); optical properties (reflectance, transmittance, absorptance); chlorophyll and carotenoid content; specific leaf mass; carbon (C) and nitrogen (N) content; fluorescence emission spectra at multiple excitation wavelengths; the ChlF contribution to red (R) and far-red (FR) reflectance; fluorescence imagery; and fluorescence excitation-emission matrices (EEMs). The tree species examined were tulip poplar (Liriodendron tulipifera L.), red maple (Acer rubrum L.), and sweetgum (Liquidambar styraczflua L.), and each had been provided four levels of N augmentation (0, 19, 37, and 75 kg Nhectare seasonally) to simulate atmospheric deposition from air pollution. Whole-plant SIF measurements of these species were compared with SIF

  8. Soil respiration in the cold desert environment of the Colorado Plateau (USA): Abiotic regulators and thresholds

    USGS Publications Warehouse

    Fernandez, D.P.; Neff, J.C.; Belnap, J.; Reynolds, R.L.

    2006-01-01

    Decomposition is central to understanding ecosystem carbon exchange and nutrient-release processes. Unlike mesic ecosystems, which have been extensively studied, xeric landscapes have received little attention; as a result, abiotic soil-respiration regulatory processes are poorly understood in xeric environments. To provide a more complete and quantitative understanding about how abiotic factors influence soil respiration in xeric ecosystems, we conducted soil- respiration and decomposition-cloth measurements in the cold desert of southeast Utah. Our study evaluated when and to what extent soil texture, moisture, temperature, organic carbon, and nitrogen influence soil respiration and examined whether the inverse-texture hypothesis applies to decomposition. Within our study site, the effect of texture on moisture, as described by the inverse texture hypothesis, was evident, but its effect on decomposition was not. Our results show temperature and moisture to be the dominant abiotic controls of soil respiration. Specifically, temporal offsets in temperature and moisture conditions appear to have a strong control on soil respiration, with the highest fluxes occurring in spring when temperature and moisture were favorable. These temporal offsets resulted in decomposition rates that were controlled by soil moisture and temperature thresholds. The highest fluxes of CO2 occurred when soil temperature was between 10 and 16??C and volumetric soil moisture was greater than 10%. Decomposition-cloth results, which integrate decomposition processes across several months, support the soil-respiration results and further illustrate the seasonal patterns of high respiration rates during spring and low rates during summer and fall. Results from this study suggest that the parameters used to predict soil respiration in mesic ecosystems likely do not apply in cold-desert environments. ?? Springer 2006.

  9. ATS-6 - Flight performance of the Advanced Thermal Control Flight Experiment

    NASA Technical Reports Server (NTRS)

    Kirkpatrick, J. P.; Brennan, P. J.

    1975-01-01

    The Advanced Thermal Control Flight Experiment on ATS-6 was designed to demonstrate the thermal control capability of a thermal diode (one-way) heat pipe, a phase-change material for thermal storage, and a feedback-controlled heat pipe. Flight data for the different operational modes are compared to ground test data, and the performance of the components is evaluated on an individual basis and as an integrated temperature-control system.

  10. Active structural control design and experiment for the Mini-Mast

    NASA Technical Reports Server (NTRS)

    Wie, Bong; Horta, Lucas; Sulla, Jeff

    1990-01-01

    Control system design and closed-loop test results for the Mini-Mast truss structure located at the NASA Langley Research Center are presented. The simplicity and effectiveness of a classical control approach to the active structural control design are demonstrated by ground experiments. The concepts of robust nonminimum phase compensation and periodic disturbance rejection are also experimentally validated. The practicality of a sensor output decoupling approach is demonstrated for the inherent, multivariable control problem of the Mini-Mast.

  11. A negative association between video game experience and proactive cognitive control.

    PubMed

    Bailey, Kira; West, Robert; Anderson, Craig A

    2010-01-01

    Some evidence demonstrates that video game experience has a beneficial effect on visuospatial cognition. In contrast, other evidence indicates that video game experience may be negatively related to cognitive control. In this study we examined the specificity of the influence of video game experience on cognitive control. Participants with high and low video game experience performed the Stroop task while event-related brain potentials were recorded. The behavioral data revealed no difference between high and low gamers for the Stroop interference effect and a reduction in the conflict adaptation effect in high gamers. The amplitude of the medial frontal negativity and a frontal slow wave was attenuated in high gamers, and there was no effect of gaming status on the conflict slow potential. These data lead to the suggestion that video game experience has a negative influence on proactive, but not reactive, cognitive control.

  12. Integrating omic approaches for abiotic stress tolerance in soybean

    PubMed Central

    Deshmukh, Rupesh; Sonah, Humira; Patil, Gunvant; Chen, Wei; Prince, Silvas; Mutava, Raymond; Vuong, Tri; Valliyodan, Babu; Nguyen, Henry T.

    2014-01-01

    Soybean production is greatly influenced by abiotic stresses imposed by environmental factors such as drought, water submergence, salt, and heavy metals. A thorough understanding of plant response to abiotic stress at the molecular level is a prerequisite for its effective management. The molecular mechanism of stress tolerance is complex and requires information at the omic level to understand it effectively. In this regard, enormous progress has been made in the omics field in the areas of genomics, transcriptomics, and proteomics. The emerging field of ionomics is also being employed for investigating abiotic stress tolerance in soybean. Omic approaches generate a huge amount of data, and adequate advancements in computational tools have been achieved for effective analysis. However, the integration of omic-scale information to address complex genetics and physiological questions is still a challenge. In this review, we have described advances in omic tools in the view of conventional and modern approaches being used to dissect abiotic stress tolerance in soybean. Emphasis was given to approaches such as quantitative trait loci (QTL) mapping, genome-wide association studies (GWAS), and genomic selection (GS). Comparative genomics and candidate gene approaches are also discussed considering identification of potential genomic loci, genes, and biochemical pathways involved in stress tolerance mechanism in soybean. This review also provides a comprehensive catalog of available online omic resources for soybean and its effective utilization. We have also addressed the significance of phenomics in the integrated approaches and recognized high-throughput multi-dimensional phenotyping as a major limiting factor for the improvement of abiotic stress tolerance in soybean. PMID:24917870

  13. Reactive oxygen species signaling in plants under abiotic stress.

    PubMed

    Choudhury, Shuvasish; Panda, Piyalee; Sahoo, Lingaraj; Panda, Sanjib Kumar

    2013-04-01

    Abiotic stresses like heavy metals, drought, salt, low temperature, etc. are the major factors that limit crop productivity and yield. These stresses are associated with production of certain deleterious chemical entities called reactive oxygen species (ROS), which include hydrogen peroxide (H₂O₂), superoxide radical (O₂(-)), hydroxyl radical (OH(-)), etc. ROS are capable of inducing cellular damage by degradation of proteins, inactivation of enzymes, alterations in the gene and interfere in various pathways of metabolic importance. Our understanding on ROS in response to abiotic stress is revolutionized with the advancements in plant molecular biology, where the basic understanding on chemical behavior of ROS is better understood. Understanding the molecular mechanisms involved in ROS generation and its potential role during abiotic stress is important to identify means by which plant growth and metabolism can be regulated under acute stress conditions. ROS mediated oxidative stress, which is the key to understand stress related toxicity have been widely studied in many plants and the results in those studies clearly revealed that oxidative stress is the main symptom of toxicity. Plants have their own antioxidant defense mechanisms to encounter ROS that is of enzymic and non-enzymic nature . Coordinated activities of these antioxidants regulate ROS detoxification and reduces oxidative load in plants. Though ROS are always regarded to impart negative impact on plants, some reports consider them to be important in regulating key cellular functions; however, such reports in plant are limited. Molecular approaches to understand ROS metabolism and signaling have opened new avenues to comprehend its critical role in abiotic stress. ROS also acts as secondary messenger that signals key cellular functions like cell proliferation, apoptosis and necrosis. In higher eukaryotes, ROS signaling is not fully understood. In this review we summarize our understanding on ROS

  14. Abiotic ozone and oxygen in atmospheres similar to prebiotic Earth

    SciTech Connect

    Domagal-Goldman, Shawn D.; Segura, Antígona; Claire, Mark W.; Robinson, Tyler D.; Meadows, Victoria S.

    2014-09-10

    The search for life on planets outside our solar system will use spectroscopic identification of atmospheric biosignatures. The most robust remotely detectable potential biosignature is considered to be the detection of oxygen (O{sub 2}) or ozone (O{sub 3}) simultaneous to methane (CH{sub 4}) at levels indicating fluxes from the planetary surface in excess of those that could be produced abiotically. Here we use an altitude-dependent photochemical model with the enhanced lower boundary conditions necessary to carefully explore abiotic O{sub 2} and O{sub 3} production on lifeless planets with a wide variety of volcanic gas fluxes and stellar energy distributions. On some of these worlds, we predict limited O{sub 2} and O{sub 3} buildup, caused by fast chemical production of these gases. This results in detectable abiotic O{sub 3} and CH{sub 4} features in the UV-visible, but no detectable abiotic O{sub 2} features. Thus, simultaneous detection of O{sub 3} and CH{sub 4} by a UV-visible mission is not a strong biosignature without proper contextual information. Discrimination between biological and abiotic sources of O{sub 2} and O{sub 3} is possible through analysis of the stellar and atmospheric context—particularly redox state and O atom inventory—of the planet in question. Specifically, understanding the spectral characteristics of the star and obtaining a broad wavelength range for planetary spectra should allow more robust identification of false positives for life. This highlights the importance of wide spectral coverage for future exoplanet characterization missions. Specifically, discrimination between true and false positives may require spectral observations that extend into infrared wavelengths and provide contextual information on the planet's atmospheric chemistry.

  15. An HP 85 Microcomputer for Experiment Control in the Physics Laboratory.

    ERIC Educational Resources Information Center

    Walrand, Jacques; And Others

    1983-01-01

    Describes a simple and inexpensive interface to be used for data acquisition and control of some experiments in an undergraduate physics laboratory. Two specific applications are presented. Seven references are listed. (Author/MBR)

  16. How much control is enough? Influence of unreliable input on user experience.

    PubMed

    van de Laar, Bram; Plass-Oude Bos, Danny; Reuderink, Boris; Poel, Mannes; Nijholt, Anton

    2013-12-01

    Brain–computer interfaces (BCI) provide a valuable new input modality within human–computer interaction systems. However, like other body-based inputs such as gesture or gaze based systems, the system recognition of input commands is still far from perfect. This raises important questions, such as what level of control should such an interface be able to provide. What is the relationship between actual and perceived control? And in the case of applications for entertainment in which fun is an important part of user experience, should we even aim for the highest level of control, or is the optimum elsewhere? In this paper, we evaluate whether we can modulate the amount of control and if a game can be fun with less than perfect control. In the experiment users (n = 158) played a simple game in which a hamster has to be guided to the exit of a maze. The amount of control the user has over the hamster is varied. The variation of control through confusion matrices makes it possible to simulate the experience of using a BCI, while using the traditional keyboard for input. After each session the user completed a short questionnaire on user experience and perceived control. Analysis of the data showed that the perceived control of the user could largely be explained by the amount of control in the respective session. As expected, user frustration decreases with increasing control. Moreover, the results indicate that the relation between fun and control is not linear. Although at lower levels of control fun does increase with improved control, the level of fun drops just before perfect control is reached (with an optimum around 96%). This poses new insights for developers of games who want to incorporate some form of BCI or other modality with unreliable input in their game: for creating a fun game, unreliable input can be used to create a challenge for the user.

  17. F-16XL-2 Supersonic Laminar Flow Control Flight Test Experiment

    NASA Technical Reports Server (NTRS)

    Anders, Scott G.; Fischer, Michael C.

    1999-01-01

    The F-16XL-2 Supersonic Laminar Flow Control Flight Test Experiment was part of the NASA High-Speed Research Program. The goal of the experiment was to demonstrate extensive laminar flow, to validate computational fluid dynamics (CFD) codes and design methodology, and to establish laminar flow control design criteria. Topics include the flight test hardware and design, airplane modification, the pressure and suction distributions achieved, the laminar flow achieved, and the data analysis and code correlation.

  18. The human operator in manual preview tracking /an experiment and its modeling via optimal control/

    NASA Technical Reports Server (NTRS)

    Tomizuka, M.; Whitney, D. E.

    1976-01-01

    A manual preview tracking experiment and its results are presented. The preview drastically improves the tracking performance compared to zero-preview tracking. Optimal discrete finite preview control is applied to determine the structure of a mathematical model of the manual preview tracking experiment. Variable parameters in the model are adjusted to values which are consistent to the published data in manual control. The model with the adjusted parameters is found to be well correlated to the experimental results.

  19. Assessing Prior Experience in the Selection of Air Traffic Control Specialists

    DTIC Science & Technology

    2013-04-01

    Crosstabulation --------------------------B1 APPENDIX C: IFR Operations Experience • Academy Training Performance Crosstabulation ---------C1 APPENDIX...Control Specialist (ATCS) rating? (n=9,333) BQ35 – Do you have prior Instrument Flight Rules ( IFR ) operations experience? (n = 9,349) 2. Hold or...not have a prior ATCS rating. Do you have prior IFR (Instrument Flight Rules) Operations experience? Of the 9,349 respondents to this question

  20. An application of the IMC software to controller design for the JPL LSCL Experiment Facility

    NASA Technical Reports Server (NTRS)

    Zhu, Guoming; Skelton, Robert E.

    1993-01-01

    A software package which Integrates Model reduction and Controller design (The IMC software) is applied to design controllers for the JPL Large Spacecraft Control Laboratory Experiment Facility. Modal Cost Analysis is used for the model reduction, and various Output Covariance Constraints are guaranteed by the controller design. The main motivation is to find the controller with the 'best' performance with respect to output variances. Indeed it is shown that by iterating on the reduced order design model, the controller designed does have better performance than that obtained with the first model reduction.

  1. WebLab of a DC Motor Speed Control Didactical Experiment

    ERIC Educational Resources Information Center

    Bauer, Karine; Mendes, Luciano

    2012-01-01

    Purpose: Weblabs are an additional resource in the execution of experiments in control engineering education, making learning process more flexible both in time, by allowing extra class laboratory activities, and space, bringing the learning experience to remote locations where experimentation facilities would not be available. The purpose of this…

  2. Object-Based Control of Attention Is Sensitive to Recent Experience

    ERIC Educational Resources Information Center

    Lee, Hyunkyu; Mozer, Michael C.; Kramer, Arthur F.; Vecera, Shaun P.

    2012-01-01

    How is attention guided by past experience? In visual search, numerous studies have shown that recent trials influence responses to the current trial. Repeating features such as color, shape, or location of a target facilitates performance. Here we examine whether recent experience also modulates a more abstract dimension of attentional control,…

  3. Beyond "Witnessing": Children's Experiences of Coercive Control in Domestic Violence and Abuse.

    PubMed

    Callaghan, Jane E M; Alexander, Joanne H; Sixsmith, Judith; Fellin, Lisa Chiara

    2015-12-10

    Children's experiences and voices are underrepresented in academic literature and professional practice around domestic violence and abuse. The project "Understanding Agency and Resistance Strategies" (UNARS) addresses this absence, through direct engagement with children. We present an analysis from interviews with 21 children in the United Kingdom (12 girls and 9 boys, aged 8-18 years), about their experiences of domestic violence and abuse, and their responses to this violence. These interviews were analyzed using interpretive interactionism. Three themes from this analysis are presented: (a) "Children's experiences of abusive control," which explores children's awareness of controlling behavior by the adult perpetrator, their experience of that control, and its impact on them; (b) "Constraint," which explores how children experience the constraint associated with coercive control in situations of domestic violence; and (c) "Children as agents," which explores children's strategies for managing controlling behavior in their home and in family relationships. The article argues that, in situations where violence and abuse occur between adult intimate partners, children are significantly affected, and can be reasonably described as victims of abusive control. Recognizing children as direct victims of domestic violence and abuse would produce significant changes in the way professionals respond to them, by (a) recognizing children's experience of the impact of domestic violence and abuse; (b) recognizing children's agency, undermining the perception of them as passive "witnesses" or "collateral damage" in adult abusive encounters; and (c) strengthening professional responses to them as direct victims, not as passive witnesses to violence.

  4. Mechanization of and experience with a triplex fly-by-wire backup control system

    NASA Technical Reports Server (NTRS)

    Lock, W. P.; Petersen, W. R.; Whitman, G. B.

    1975-01-01

    A redundant three-axis analog control system was designed and developed to back up a digital fly-by-wire control system for an F-8C airplane. Forty-two flights, involving 58 hours of flight time, were flown by six pilots. The mechanization and operational experience with the backup control system, the problems involved in synchronizing it with the primary system, and the reliability of the system are discussed. The backup control system was dissimilar to the primary system, and it provided satisfactory handling through the flight envelope evaluated. Limited flight tests of a variety of control tasks showed that control was also satisfactory when the backup control system was controlled by a minimum-displacement (force) side stick. The operational reliability of the F-8 digital fly-by-wire control system was satisfactory, with no unintentional downmodes to the backup control system in flight. The ground and flight reliability of the system's components is discussed.

  5. Mechanization of and experience with a triplex fly-by-wire backup control system

    NASA Technical Reports Server (NTRS)

    Lock, W. P.; Petersen, W. R.; Whitman, G. B.

    1976-01-01

    A redundant three axis analog control system was designed and developed to back up a digital fly by wire control system for an F-8C airplane. The mechanization and operational experience with the backup control system, the problems involved in synchronizing it with the primary system, and the reliability of the system are discussed. The backup control system was dissimilar to the primary system, and it provided satisfactory handling through the flight envelope evaluated. Limited flight tests of a variety of control tasks showed that control was also satisfactory when the backup control system was controlled by a minimum displacement (force) side stick. The operational reliability of the F-8 digital fly by wire control system was satisfactory, with no unintentional downmodes to the backup control system in flight. The ground and flight reliability of the system's components is discussed.

  6. Development of operator requested control system applications: Experience with the SLC control system at SLAC

    SciTech Connect

    Stanek, M.

    1995-12-01

    The SLC Control system at SLAC has evolved into a powerful tool for operation of the accelerator and for troubleshooting the unique problems encountered in extracting maximum performance from the SLC. The evolution has included the development of many custom applications and user interface features generated from accelerator operator and accelerator physicist requests. These applications are written and maintained primarily by the Controls Software Engineering group, and not by the users themselves. The process of developing and supporting user requested control systems applications at SLAC is described, including the effects of organizational structure, formal and informal procedures, and control system architecture.

  7. Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses

    PubMed Central

    Garg, Ajay K.; Kim, Ju-Kon; Owens, Thomas G.; Ranwala, Anil P.; Choi, Yang Do; Kochian, Leon V.; Wu, Ray J.

    2002-01-01

    Trehalose is a nonreducing disaccharide of glucose that functions as a compatible solute in the stabilization of biological structures under abiotic stress in bacteria, fungi, and invertebrates. With the notable exception of the desiccation-tolerant “resurrection plants,” trehalose is not thought to accumulate to detectable levels in most plants. We report here the regulated overexpression of Escherichia coli trehalose biosynthetic genes (otsA and otsB) as a fusion gene for manipulating abiotic stress tolerance in rice. The fusion gene has the advantages of necessitating only a single transformation event and a higher net catalytic efficiency for trehalose formation. The expression of the transgene was under the control of either tissue-specific or stress-dependent promoters. Compared with nontransgenic rice, several independent transgenic lines exhibited sustained plant growth, less photo-oxidative damage, and more favorable mineral balance under salt, drought, and low-temperature stress conditions. Depending on growth conditions, the transgenic rice plants accumulate trehalose at levels 3–10 times that of the nontransgenic controls. The observation that peak trehalose levels remain well below 1 mg/g fresh weight indicates that the primary effect of trehalose is not as a compatible solute. Rather, increased trehalose accumulation correlates with higher soluble carbohydrate levels and an elevated capacity for photosynthesis under both stress and nonstress conditions, consistent with a suggested role in modulating sugar sensing and carbohydrate metabolism. These findings demonstrate the feasibility of engineering rice for increased tolerance of abiotic stress and enhanced productivity through tissue-specific or stress-dependent overproduction of trehalose. PMID:12456878

  8. Seed treatment with Trichoderma harzianum alleviates biotic, abiotic, and physiological stresses in germinating seeds and seedlings.

    PubMed

    Mastouri, Fatemeh; Björkman, Thomas; Harman, Gary E

    2010-11-01

    Trichoderma spp. are endophytic plant symbionts that are widely used as seed treatments to control diseases and to enhance plant growth and yield. Although some recent work has been published on their abilities to alleviate abiotic stresses, specific knowledge of mechanisms, abilities to control multiple plant stress factors, their effects on seed and seedlings is lacking. We examined the effects of seed treatment with T. harzianum strain T22 on germination of seed exposed to biotic stress (seed and seedling disease caused by Pythium ultimum) and abiotic stresses (osmotic, salinity, chilling, or heat stress). We also evaluated the ability of the beneficial fungus to overcome physiological stress (poor seed quality induced by seed aging). If seed were not under any of the stresses noted above, T22 generally had little effect upon seedling performance. However, under stress, treated seed germinated consistently faster and more uniformly than untreated seeds whether the stress was osmotic, salt, or suboptimal temperatures. The consistent response to varying stresses suggests a common mechanism through which the plant-fungus association enhances tolerance to a wide range of abiotic stresses as well as biotic stress. A common factor that negatively affects plants under these stress conditions is accumulation of toxic reactive oxygen species (ROS), and we tested the hypothesis that T22 reduced damages resulting from accumulation of ROS in stressed plants. Treatment of seeds reduced accumulation of lipid peroxides in seedlings under osmotic stress or in aged seeds. In addition, we showed that the effect of exogenous application of an antioxidant, glutathione, or application of T22, resulted in a similar positive effect on seed germination under osmotic stress or in aged seed. This evidence supports the model that T. harzianum strain T22 increases seedling vigor and ameliorates stress by inducing physiological protection in plants against oxidative damage.

  9. Flight Simulator Experiments on Influence of Wideness of Front View for Pilot's Roll Control

    NASA Astrophysics Data System (ADS)

    Kumata, Kazunari; Nishihata, Michiteru; Kobayashi, Osamu

    Fixed based flight simulator experiments were conducted to investigate the influences of wideness of front view for pilot's roll control. In these experiments, the airplane's motion was considered as a single-degree-of-freedom system in roll, and three front views having different view-angle were provided. The results of these experiments showed that the pilot's roll control characteristics, and the pilot's sensing parameter and reaction time for rolling motion were influenced by the differences of wideness of front view in flight simulator.

  10. The Intelligent Control System and Experiments for an Unmanned Wave Glider

    PubMed Central

    Liao, Yulei; Wang, Leifeng; Li, Yiming; Li, Ye; Jiang, Quanquan

    2016-01-01

    The control system designing of Unmanned Wave Glider (UWG) is challenging since the control system is weak maneuvering, large time-lag and large disturbance, which is difficult to establish accurate mathematical model. Meanwhile, to complete marine environment monitoring in long time scale and large spatial scale autonomously, UWG asks high requirements of intelligence and reliability. This paper focuses on the “Ocean Rambler” UWG. First, the intelligent control system architecture is designed based on the cerebrum basic function combination zone theory and hierarchic control method. The hardware and software designing of the embedded motion control system are mainly discussed. A motion control system based on rational behavior model of four layers is proposed. Then, combining with the line-of sight method(LOS), a self-adapting PID guidance law is proposed to compensate the steady state error in path following of UWG caused by marine environment disturbance especially current. Based on S-surface control method, an improved S-surface heading controller is proposed to solve the heading control problem of the weak maneuvering carrier under large disturbance. Finally, the simulation experiments were carried out and the UWG completed autonomous path following and marine environment monitoring in sea trials. The simulation experiments and sea trial results prove that the proposed intelligent control system, guidance law, controller have favorable control performance, and the feasibility and reliability of the designed intelligent control system of UWG are verified. PMID:28005956

  11. Interactive effects of MnO2, organic matter and pH on abiotic formation of N2O from hydroxylamine in artificial soil mixtures.

    PubMed

    Liu, Shurong; Berns, Anne E; Vereecken, Harry; Wu, Di; Brüggemann, Nicolas

    2017-02-01

    Abiotic conversion of the reactive nitrification intermediate hydroxylamine (NH2OH) to nitrous oxide (N2O) is a possible mechanism of N2O formation during nitrification. Previous research has demonstrated that manganese dioxide (MnO2) and organic matter (OM) content of soil as well as soil pH are important control variables of N2O formation in the soil. But until now, their combined effect on abiotic N2O formation from NH2OH has not been quantified. Here, we present results from a full-factorial experiment with artificial soil mixtures at five different levels of pH, MnO2 and OM, respectively, and quantified the interactive effects of the three variables on the NH2OH-to-N2O conversion ratio (RNH2OH-to-N2O). Furthermore, the effect of OM quality on RNH2OH-to-N2O was determined by the addition of four different organic materials with different C/N ratios to the artificial soil mixtures. The experiments revealed a strong interactive effect of soil pH, MnO2 and OM on RNH2OH-to-N2O. In general, increasing MnO2 and decreasing pH increased RNH2OH-to-N2O, while increasing OM content was associated with a decrease in RNH2OH-to-N2O. Organic matter quality also affected RNH2OH-to-N2O. However, this effect was not a function of C/N ratio, but was rather related to differences in the dominating functional groups between the different organic materials.

  12. Interactive effects of MnO2, organic matter and pH on abiotic formation of N2O from hydroxylamine in artificial soil mixtures

    NASA Astrophysics Data System (ADS)

    Liu, Shurong; Berns, Anne E.; Vereecken, Harry; Wu, Di; Brüggemann, Nicolas

    2017-02-01

    Abiotic conversion of the reactive nitrification intermediate hydroxylamine (NH2OH) to nitrous oxide (N2O) is a possible mechanism of N2O formation during nitrification. Previous research has demonstrated that manganese dioxide (MnO2) and organic matter (OM) content of soil as well as soil pH are important control variables of N2O formation in the soil. But until now, their combined effect on abiotic N2O formation from NH2OH has not been quantified. Here, we present results from a full-factorial experiment with artificial soil mixtures at five different levels of pH, MnO2 and OM, respectively, and quantified the interactive effects of the three variables on the NH2OH-to-N2O conversion ratio (RNH2OH-to-N2O). Furthermore, the effect of OM quality on RNH2OH-to-N2O was determined by the addition of four different organic materials with different C/N ratios to the artificial soil mixtures. The experiments revealed a strong interactive effect of soil pH, MnO2 and OM on RNH2OH-to-N2O. In general, increasing MnO2 and decreasing pH increased RNH2OH-to-N2O, while increasing OM content was associated with a decrease in RNH2OH-to-N2O. Organic matter quality also affected RNH2OH-to-N2O. However, this effect was not a function of C/N ratio, but was rather related to differences in the dominating functional groups between the different organic materials.

  13. Interactive effects of MnO2, organic matter and pH on abiotic formation of N2O from hydroxylamine in artificial soil mixtures

    PubMed Central

    Liu, Shurong; Berns, Anne E.; Vereecken, Harry; Wu, Di; Brüggemann, Nicolas

    2017-01-01

    Abiotic conversion of the reactive nitrification intermediate hydroxylamine (NH2OH) to nitrous oxide (N2O) is a possible mechanism of N2O formation during nitrification. Previous research has demonstrated that manganese dioxide (MnO2) and organic matter (OM) content of soil as well as soil pH are important control variables of N2O formation in the soil. But until now, their combined effect on abiotic N2O formation from NH2OH has not been quantified. Here, we present results from a full-factorial experiment with artificial soil mixtures at five different levels of pH, MnO2 and OM, respectively, and quantified the interactive effects of the three variables on the NH2OH-to-N2O conversion ratio (RNH2OH-to-N2O). Furthermore, the effect of OM quality on RNH2OH-to-N2O was determined by the addition of four different organic materials with different C/N ratios to the artificial soil mixtures. The experiments revealed a strong interactive effect of soil pH, MnO2 and OM on RNH2OH-to-N2O. In general, increasing MnO2 and decreasing pH increased RNH2OH-to-N2O, while increasing OM content was associated with a decrease in RNH2OH-to-N2O. Organic matter quality also affected RNH2OH-to-N2O. However, this effect was not a function of C/N ratio, but was rather related to differences in the dominating functional groups between the different organic materials. PMID:28145407

  14. Biogeomorphology of a Mojave Desert landscape - Configurations and feedbacks of abiotic and biotic land surfaces during landform evolution

    NASA Astrophysics Data System (ADS)

    Pietrasiak, Nicole; Drenovsky, Rebecca E.; Santiago, Louis S.; Graham, Robert C.

    2014-02-01

    Terrestrial ecosystems can be more holistically understood by investigating the morphology of landscape mosaics, the assemblage of their ecological communities, and the linkages and feedbacks between the mosaics and communities. The overarching objectives of this study were to: (1) study the abiotic and biotic configurations of landform units as mosaics within a Mojave Desert chronosequence; and (2) elucidate their potential feedbacks, interactions, and dynamics during landform evolution. Seven landform units distributed over three geomorphic ages were identified, including: young bars and swales; intermediate-aged flattened bars, flattened swales, and bioturbation units; and old desert pavements and shrub zones. These landform units were characterized according to abiotic and biotic land surface properties. Landform units were statistically distinct and predictable based on a specific suite of abiotic and biotic properties. Vascular plant functional group and biological soil crust community diversity varied with geomorphology, with greatest diversity associated with bars and shrub zones and lowest diversity associated with desert pavements. Biological soil crust communities were controlled by geomorphic age, surface rock size, and protruding rocks with young bar units having the highest abundance and diversity. Perennial forbs were observed in old shrub zones with small rocks and few protruding rocks. A high clast density and a finer-sized clast distribution were found particularly in desert pavements and flattened swales, and generally inhibited biological soil crust and plant cover. Evolutionary trajectories for landforms of a lower piedmont landscape can be dominated by either abiotic and biotic landform processes. These two trajectories are distinctly different and are associated with their own unique linkages, feedbacks, and dynamics of abiotic and biotic land surface properties, producing a highly diverse desert landscape.

  15. Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis.

    PubMed

    Nakai, Yusuke; Nakahira, Yoichi; Sumida, Hiroki; Takebayashi, Kosuke; Nagasawa, Yumiko; Yamasaki, Kanako; Akiyama, Masako; Ohme-Takagi, Masaru; Fujiwara, Sumire; Shiina, Takashi; Mitsuda, Nobutaka; Fukusaki, Eiichiro; Kubo, Yasuyuki; Sato, Masa H

    2013-03-01

    Plants adapt to abiotic and biotic stresses by activating abscisic acid-mediated (ABA) abiotic stress-responsive and salicylic acid-(SA) or jasmonic acid-mediated (JA) biotic stress-responsive pathways, respectively. Although the abiotic stress-responsive pathway interacts antagonistically with the biotic stress-responsive pathways, the mechanisms that regulate these pathways remain largely unknown. In this study, we provide insight into the function of vascular plant one-zinc-finger proteins (VOZs) that modulate various stress responses in Arabidopsis. The expression of many stress-responsive genes was changed in the voz1voz2 double mutant under normal growth conditions. Consistent with altered stress-responsive gene expression, freezing- and drought-stress tolerances were increased in the voz1voz2 double mutant. In contrast, resistance to a fungal pathogen, Colletotrichum higginsianum, and to a bacterial pathogen, Pseudomonas syringae, was severely impaired. Thus, impairing VOZ function simultaneously conferred increased abiotic tolerance and biotic stress susceptibility. In a chilling stress condition, both the VOZ1 and VOZ2 mRNA expression levels and the VOZ2 protein level gradually decreased. VOZ2 degradation during cold exposure was completely inhibited by the addition of the 26S proteasome inhibitor, MG132, a finding that suggested that VOZ2 degradation is dependent on the ubiquitin/26S proteasome system. In voz1voz2, ABA-inducible transcription factor CBF4 expression was enhanced significantly even under normal growth conditions, despite an unchanged endogenous ABA content. A finding that suggested that VOZs negatively affect CBF4 expression in an ABA-independent manner. These results suggest that VOZs function as both negative and positive regulators of the abiotic and biotic stress-responsive pathways, and control Arabidopsis adaptation to various stress conditions.

  16. Flexible structure control experiments using a real-time workstation for computer-aided control engineering

    NASA Technical Reports Server (NTRS)

    Stieber, Michael E.

    1989-01-01

    A Real-Time Workstation for Computer-Aided Control Engineering has been developed jointly by the Communications Research Centre (CRC) and Ruhr-Universitaet Bochum (RUB), West Germany. The system is presently used for the development and experimental verification of control techniques for large space systems with significant structural flexibility. The Real-Time Workstation essentially is an implementation of RUB's extensive Computer-Aided Control Engineering package KEDDC on an INTEL micro-computer running under the RMS real-time operating system. The portable system supports system identification, analysis, control design and simulation, as well as the immediate implementation and test of control systems. The Real-Time Workstation is currently being used by CRC to study control/structure interaction on a ground-based structure called DAISY, whose design was inspired by a reflector antenna. DAISY emulates the dynamics of a large flexible spacecraft with the following characteristics: rigid body modes, many clustered vibration modes with low frequencies and extremely low damping. The Real-Time Workstation was found to be a very powerful tool for experimental studies, supporting control design and simulation, and conducting and evaluating tests withn one integrated environment.

  17. Birth control sabotage and forced sex: experiences reported by women in domestic violence shelters.

    PubMed

    Thiel de Bocanegra, Heike; Rostovtseva, Daria P; Khera, Satin; Godhwani, Nita

    2010-05-01

    Women who experience intimate partner violence often experience birth control sabotage, forced sex, and partner's unwillingness to use condoms. We interviewed 53 women at four domestic violence shelters. Participants reported that their abusive partners frequently refused to use condoms, impeded them from accessing health care, and subjected them to birth control sabotage, infidelity, and forced sex. However, women also reported strategies to counteract these actions, particularly against birth control sabotage and attempts to force them to abort or continue a pregnancy. Domestic violence counselors can focus on these successful strategies to validate coping skills and build self-esteem.

  18. CSM digital autopilot testing in support of ASTP experiments control requirements

    NASA Technical Reports Server (NTRS)

    Rue, D. L.

    1975-01-01

    Results are presented of CSM digital autopilot (DAP) testing. The testing was performed to demonstrate and evaluate control modes which are currently planned or could be considered for use in support of experiments on the ASTP mission. The testing was performed on the Lockheed Guidance, Navigation, and Control System Functional Simulator (GNCFS). This simulator, which was designed to test the Apollo and Skylab DAP control system, has been used extensively and is a proven tool for CSM DAP analysis.

  19. Upgrades for the STAR Experiment Slow Controls at Brookhaven National Laboratory

    NASA Astrophysics Data System (ADS)

    Ruiz, Samuel; Fujita, Jiro

    2016-09-01

    The STAR (Solenoidal Tracker at RHIC) experiment at Brookhaven National Laboratory studies the collisions of relativistic heavy ions. Some components of the control system for STAR have not changed since STAR became operational in 2000. The goal of this project is to upgrade the control system software for easier maintenance. The software upgrade also requires modernizing the hardware in some cases. A prototype for monitoring the Field Cage for the Time Projection Chamber (TPC) and a prototype for controlling the TPC front-end electronics using a programmable logic controller were developed. Newly developed software for the hygrometer to monitor the experiment environment was deployed at the experiment. Details about the software and hardware upgrades, as well as the developed prototypes will be presented.

  20. Construction of AC Motor Controllers for NOvA Experiment Upgrades

    SciTech Connect

    Cooley, Patrick; /Fermilab

    2011-08-04

    I have been constructing Alternating Current (AC) motor controllers for manipulation of particle beam detectors. The capability and reliability of these motor controllers are essential to the Laboratory's mission of accurate analysis of the particle beam's position. The device is moved in and out of the beam's path by the motor controller followed by the Neutrinos at the Main Injector Off-Axis {nu}{sub e} Appearance (NOvA) Experiment further down the beam pipe. In total, I built and tested ten ac motor controllers for new beam operations in the NOvA experiment. These units will prove to be durable and provide extremely accurate beam placement for NOvA Experiment far into the future.

  1. Hormone balance and abiotic stress tolerance in crop plants.

    PubMed

    Peleg, Zvi; Blumwald, Eduardo

    2011-06-01

    Plant hormones play central roles in the ability of plants to adapt to changing environments, by mediating growth, development, nutrient allocation, and source/sink transitions. Although ABA is the most studied stress-responsive hormone, the role of cytokinins, brassinosteroids, and auxins during environmental stress is emerging. Recent evidence indicated that plant hormones are involved in multiple processes. Cross-talk between the different plant hormones results in synergetic or antagonic interactions that play crucial roles in response of plants to abiotic stress. The characterization of the molecular mechanisms regulating hormone synthesis, signaling, and action are facilitating the modification of hormone biosynthetic pathways for the generation of transgenic crop plants with enhanced abiotic stress tolerance.

  2. Abiotic mediation of a mutualism drives herbivore abundance.

    PubMed

    Mooney, Emily H; Phillips, Joseph S; Tillberg, Chadwick V; Sandrow, Cheryl; Nelson, Annika S; Mooney, Kailen A

    2016-01-01

    Species abundance is typically determined by the abiotic environment, but the extent to which such effects occur through the mediation of biotic interactions, including mutualisms, is unknown. We explored how light environment (open meadow vs. shaded understory) mediates the abundance and ant tending of the aphid Aphis helianthi feeding on the herb Ligusticum porteri. Yearly surveys consistently found aphids to be more than 17-fold more abundant on open meadow plants than on shaded understory plants. Manipulations demonstrated that this abundance pattern was not due to the direct effects of light environment on aphid performance, or indirectly through host plant quality or the effects of predators. Instead, open meadows had higher ant abundance and per capita rates of aphid tending and, accordingly, ants increased aphid population growth in meadow but not understory environments. The abiotic environment thus drives the abundance of this herbivore exclusively through the mediation of a protection mutualism.

  3. Abiotic pyrite formation produces a large Fe isotope fractionation.

    PubMed

    Guilbaud, Romain; Butler, Ian B; Ellam, Rob M

    2011-06-24

    The iron isotope composition of sedimentary pyrite has been proposed as a potential proxy to trace microbial metabolism and the redox evolution of the oceans. We demonstrate that Fe isotope fractionation accompanies abiotic pyrite formation in the absence of Fe(II) redox change. Combined fractionation factors between Fe(II)(aq), mackinawite, and pyrite permit the generation of pyrite with Fe isotope signatures that nearly encapsulate the full range of sedimentary δ(56)Fe(pyrite) recorded in Archean to modern sediments. We propose that Archean negative Fe isotope excursions reflect partial Fe(II)(aq) utilization during abiotic pyrite formation rather than microbial dissimilatory Fe(III) reduction. Late Proterozoic to modern sediments may reflect greater Fe(II)(aq) utilization and variations in source composition.

  4. Transcriptional networks-crops, clocks, and abiotic stress.

    PubMed

    Gehan, Malia A; Greenham, Kathleen; Mockler, Todd C; McClung, C Robertson

    2015-04-01

    Several factors affect the yield potential and geographical range of crops including the circadian clock, water availability, and seasonal temperature changes. In order to sustain and increase plant productivity on marginal land in the face of both biotic and abiotic stresses, we need to more efficiently generate stress-resistant crops through marker-assisted breeding, genetic modification, and new genome-editing technologies. To leverage these strategies for producing the next generation of crops, future transcriptomic data acquisition should be pursued with an appropriate temporal design and analyzed with a network-centric approach. The following review focuses on recent developments in abiotic stress transcriptional networks in economically important crops and will highlight the utility of correlation-based network analysis and applications.

  5. Arbuscular mycorrhizal fungal responses to abiotic stresses: A review.

    PubMed

    Lenoir, Ingrid; Fontaine, Joël; Lounès-Hadj Sahraoui, Anissa

    2016-03-01

    The majority of plants live in close collaboration with a diversity of soil organisms among which arbuscular mycorrhizal fungi (AMF) play an essential role. Mycorrhizal symbioses contribute to plant growth and plant protection against various environmental stresses. Whereas the resistance mechanisms induced in mycorrhizal plants after exposure to abiotic stresses, such as drought, salinity and pollution, are well documented, the knowledge about the stress tolerance mechanisms implemented by the AMF themselves is limited. This review provides an overview of the impacts of various abiotic stresses (pollution, salinity, drought, extreme temperatures, CO2, calcareous, acidity) on biodiversity, abundance and development of AMF and examines the morphological, biochemical and molecular mechanisms implemented by AMF to survive in the presence of these stresses.

  6. Fast Abiotic Production of Methane at Temperatures Below 100°C

    NASA Astrophysics Data System (ADS)

    Etiope, G.; Ionescu, A.

    2015-12-01

    Fischer-Tropsch Type (FTT) reactions, e.g., the Sabatier synthesis between H2 and CO2, are considered a main source of abiotic methane on Earth and likely on other planets. Several laboratory FTT experiments demonstrated abiotic CH4 production at temperatures above 200°C, by using Fe, Ni or Cr catalysts, simulating hydrothermal conditions in peridotite-hosted systems in mid-ocean ridges. Nevertheless, at least on laboratory experiment time-scale, Fe-Ni-Cr catalysts do not support CH4 generation at T<100°C, such as those of land-based serpentinization systems. We have recently reported rapid production of considerable amounts of CH4 (>800 ppmv in 155 mL bottles after 1 day) via Sabatier reaction at 90, 50 and 25°C, using small concentrations of non-pretreated ruthenium (Ru) equivalent to those occurring in chromitites in continental ultramafic rocks (Etiope & Ionescu, 2014; Geofluids, doi:10.1111/gfl.12106). We have repeated the experiments by using 13C-enriched CO2 and we confirm fast production of CH4at percentage levels. The experiments performed so far show that: 1. considerable amounts of CH4can be produced in dry conditions below 100°C with small quantities of Ru; 2. under the same experimental conditions (<100°C), Fe, Ni and Cr oxides do not produce CH4; 3. low T Sabatier reaction can produce CH4 with a large C isotope fractionation between CO2 and CH4, leading to relatively " light" (13C-depleted) CH4, resembling microbial gas; 4. the CO2-CH4isotope separation decreases over time and by increasing the temperature; 5. minor amounts of C2-C6hydrocarbons are also generated. Our laboratory data are compatible with the isotopic patterns of CH4 naturally occurring in land-based seeps and springs. Our experiments suggest that Ru-enriched chromitites could potentially generate CH4 at low T. Since Ru is reported in Martian meteorites, low T abiotic CH4 production on Mars via Sabatier reaction cannot be excluded (Etiope et al. 2013, Icarus, 224, 276-285).

  7. Methylglyoxal: An Emerging Signaling Molecule in Plant Abiotic Stress Responses and Tolerance

    PubMed Central

    Hoque, Tahsina S.; Hossain, Mohammad A.; Mostofa, Mohammad G.; Burritt, David J.; Fujita, Masayuki; Tran, Lam-Son P.

    2016-01-01

    The oxygenated short aldehyde methylglyoxal (MG) is produced in plants as a by-product of a number of metabolic reactions, including elimination of phosphate groups from glycolysis intermediates dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. MG is mostly detoxified by the combined actions of the enzymes glyoxalase I and glyoxalase II that together with glutathione make up the glyoxalase system. Under normal growth conditions, basal levels of MG remain low in plants; however, when plants are exposed to abiotic stress, MG can accumulate to much higher levels. Stress-induced MG functions as a toxic molecule, inhibiting different developmental processes, including seed germination, photosynthesis and root growth, whereas MG, at low levels, acts as an important signaling molecule, involved in regulating diverse events, such as cell proliferation and survival, control of the redox status of cells, and many other aspects of general metabolism and cellular homeostases. MG can modulate plant stress responses by regulating stomatal opening and closure, the production of reactive oxygen species, cytosolic calcium ion concentrations, the activation of inward rectifying potassium channels and the expression of many stress-responsive genes. MG appears to play important roles in signal transduction by transmitting and amplifying cellular signals and functions that promote adaptation of plants growing under adverse environmental conditions. Thus, MG is now considered as a potential biochemical marker for plant abiotic stress tolerance, and is receiving considerable attention by the scientific community. In this review, we will summarize recent findings regarding MG metabolism in plants under abiotic stress, and evaluate the concept of MG signaling. In addition, we will demonstrate the importance of giving consideration to MG metabolism and the glyoxalase system, when investigating plant adaptation and responses to various environmental stresses. PMID:27679640

  8. MicroRNA: a new target for improving plant tolerance to abiotic stress

    PubMed Central

    Zhang, Baohong

    2015-01-01

    MicroRNAs (miRNAs) are an extensive class of endogenous, small RNA molecules that sit at the heart of regulating gene expression in multiple developmental and signalling pathways. Recent studies have shown that abiotic stresses induce aberrant expression of many miRNAs, thus suggesting that miRNAs may be a new target for genetically improving plant tolerance to certain stresses. These studies have also shown that miRNAs respond to environmental stresses in a miRNA-, stress-, tissue-, and genotype-dependent manner. During abiotic stress, miRNAs function by regulating target genes within the miRNA–target gene network and by controlling signalling pathways and root development. Generally speaking, stress-induced miRNAs lead to down-regulation of negative regulators of stress tolerance whereas stress-inhibited miRNAs allow the accumulation and function of positive regulators. Currently, the majority of miRNA-based studies have focused on the identification of miRNAs that are responsive to different stress conditions and analysing their expression profile changes during these treatments. This has predominately been accomplished using deep sequencing technologies and other expression analyses, such as quantitative real-time PCR. In the future, more function and expression studies will be necessary in order to elucidate the common miRNA-mediated regulatory mechanisms that underlie tolerance to different abiotic stresses. The use of artificial miRNAs, as well as overexpression and knockout/down of both miRNAs and their targets, will be the best techniques for determining the specific roles of individual miRNAs in response to environmental stresses. PMID:25697792

  9. Sustainability of Long-Term Abiotic Attenuation of Chlorinated Ethenes

    DTIC Science & Technology

    2007-09-28

    that contribute to abiotic transformations is critical to assess the feasibility of natural attenuation and promote the rationale design of...anaerobic glovebox. The resulting slurry was mixed for three days and then decanted into polypropylene centrifuge bottles. These bottles were...tightly sealed and centrifuged at 8000 rpm for 10 minutes. The supernatant in the bottles was discarded, and fresh nitrogen-purged deionized water was

  10. Biotic-Abiotic Nanoscale Interactions in Biological Fuel Cells

    DTIC Science & Technology

    2014-03-28

    Force Office of Scientific Research 875 North Randolph Street 4027 Arlington VA 22203 email: Patrick.Bradshaw@afosr.af.mil phone : 703-588-8492...Science Center 215C Los Angeles, CA 90089-0484 email: mnaggar@usc.edu phone : 213-740-2394 2 Biotic-Abiotic Nanoscale Interactions in...aggregation – collaboration with Naval Research Lab. 2.4 As part of an international collaboration, we reported on filamentous bacteria mediating centimeter

  11. Changes in biotic and abiotic processes following mangrove clearing

    NASA Astrophysics Data System (ADS)

    Granek, Elise; Ruttenberg, Benjamin I.

    2008-12-01

    Mangrove forests, important tropical coastal habitats, are in decline worldwide primarily due to removal by humans. Changes to mangrove systems can alter ecosystem properties through direct effects on abiotic factors such as temperature, light and nutrient supply or through changes in biotic factors such as primary productivity or species composition. Despite the importance of mangroves as transitional habitats between land and sea, little research has examined changes that occur when they are cleared. We examined changes in a number of biotic and abiotic factors following the anthropogenic removal of red mangroves ( Rhizophora mangle) in the Panamanian Caribbean, including algal biomass, algal diversity, algal grazing rates, light penetration, temperature, sedimentation rates and sediment organic content. In this first study examining multiple ecosystem-level effects of mangrove disturbance, we found that areas cleared of mangroves had higher algal biomass and richness than intact mangrove areas. This increase in algal biomass and richness was likely due to changes in abiotic factors (e.g. light intensity, temperature), but not biotic factors (fish herbivory). Additionally the algal and cyanobacterial genera dominating mangrove-cleared areas were rare in intact mangroves and included a number of genera that compete with coral for space on reefs. Interestingly, sedimentation rates did not differ between intact and cleared areas, but the sediments that accumulated in intact mangroves had higher organic content. These findings are the first to demonstrate that anthropogenic clearing of mangroves changes multiple biotic and abiotic processes in mangrove forests and that some of these changes may influence adjacent habitats such as coral reefs and seagrass beds. Additional research is needed to further explore the community and ecosystem-level effects of mangrove clearing and their influence on adjacent habitats, but it is clear that mangrove conservation is an

  12. Wheat EST resources for functional genomics of abiotic stress

    PubMed Central

    Houde, Mario; Belcaid, Mahdi; Ouellet, François; Danyluk, Jean; Monroy, Antonio F; Dryanova, Ani; Gulick, Patrick; Bergeron, Anne; Laroche, André; Links, Matthew G; MacCarthy, Luke; Crosby, William L; Sarhan, Fathey

    2006-01-01

    Background Wheat is an excellent species to study freezing tolerance and other abiotic stresses. However, the sequence of the wheat genome has not been completely characterized due to its complexity and large size. To circumvent this obstacle and identify genes involved in cold acclimation and associated stresses, a large scale EST sequencing approach was undertaken by the Functional Genomics of Abiotic Stress (FGAS) project. Results We generated 73,521 quality-filtered ESTs from eleven cDNA libraries constructed from wheat plants exposed to various abiotic stresses and at different developmental stages. In addition, 196,041 ESTs for which tracefiles were available from the National Science Foundation wheat EST sequencing program and DuPont were also quality-filtered and used in the analysis. Clustering of the combined ESTs with d2_cluster and TGICL yielded a few large clusters containing several thousand ESTs that were refractory to routine clustering techniques. To resolve this problem, the sequence proximity and "bridges" were identified by an e-value distance graph to manually break clusters into smaller groups. Assembly of the resolved ESTs generated a 75,488 unique sequence set (31,580 contigs and 43,908 singletons/singlets). Digital expression analyses indicated that the FGAS dataset is enriched in stress-regulated genes compared to the other public datasets. Over 43% of the unique sequence set was annotated and classified into functional categories according to Gene Ontology. Conclusion We have annotated 29,556 different sequences, an almost 5-fold increase in annotated sequences compared to the available wheat public databases. Digital expression analysis combined with gene annotation helped in the identification of several pathways associated with abiotic stress. The genomic resources and knowledge developed by this project will contribute to a better understanding of the different mechanisms that govern stress tolerance in wheat and other cereals. PMID

  13. Public-Private Partnerships for Government Financing, Controlling Risk, and Value-for-Money: The UK Experience

    DTIC Science & Technology

    2006-09-01

    unlimited. Public - private Partnerships for Government Financing, Controlling Risk, and Value-for-money: The UK Experience September 1, 2006... Public - private Partnerships for Government Financing, Controlling Risk, and Value-for- money: The UK Experience 5. FUNDING 6. AUTHOR (S... Public - private Partnerships for Government Financing, Controlling Risk, and Value-for-money: The UK Experience September 1, 2006 by Joseph G. San

  14. A membrane-bound NAC transcription factor as an integrator of biotic and abiotic stress signals.

    PubMed

    Seo, Pil Joon; Park, Chung-Mo

    2010-05-01

    Transcription factors are central components of gene regulatory networks that mediate virtually all aspects of growth and developmental processes in biological systems. The activity of transcription factors is regulated at multiple steps, such as gene transcription, posttranscriptional RNA processing, posttranslational modification, protein-protein interactions, and controlled protein turnover. Controlled activation of dormant, membrane-bound transcription factor (MTF) is an intriguing regulatory mechanism that ensures quick transcriptional responses to environmental fluctuations in plants, in which various stress hormones serve as signaling mediators. NTL6 is proteolytically activated upon exposure to cold and induces expression of the Pathogenesis-Related (PR) genes. The membrane-mediated cold signaling in inducing pathogen resistance is considered to be an adaptive strategy that protects plants against infection by hydrophilic pathogens frequently occurring during cold season. We found that NTL6 also mediates abscisic acid (ABA) regulation of abiotic stress responses in Arabidopsis. NTL6 is proteolytically activated by ABA. Transgenic plants overexpressing a nuclear NTL6 form (35S:6ΔC) exhibited a hypersensitive response to ABA and high salinity in seed germination. Taken together, these observations indicate that NTL6 plays an integrative role in plant responses to both biotic and abiotic stress conditions.

  15. Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants

    PubMed Central

    Nuruzzaman, Mohammed; Sharoni, Akhter M.; Kikuchi, Shoshi

    2013-01-01

    NAC transcription factors are one of the largest families of transcriptional regulators in plants, and members of the NAC gene family have been suggested to play important roles in the regulation of the transcriptional reprogramming associated with plant stress responses. A phylogenetic analysis of NAC genes, with a focus on rice and Arabidopsis, was performed. Herein, we present an overview of the regulation of the stress responsive NAC SNAC/(IX) group of genes that are implicated in the resistance to different stresses. SNAC factors have important roles for the control of biotic and abiotic stresses tolerance and that their overexpression can improve stress tolerance via biotechnological approaches. We also review the recent progress in elucidating the roles of NAC transcription factors in plant biotic and abiotic stresses. Modification of the expression pattern of transcription factor genes and/or changes in their activity contribute to the elaboration of various signaling pathways and regulatory networks. However, a single NAC gene often responds to several stress factors, and their protein products may participate in the regulation of several seemingly disparate processes as negative or positive regulators. Additionally, the NAC proteins function via auto-regulation or cross-regulation is extensively found among NAC genes. These observations assist in the understanding of the complex mechanisms of signaling and transcriptional reprogramming controlled by NAC proteins. PMID:24058359

  16. Application of the object-oriented paradigm for scientific experiment monitoring & control

    NASA Astrophysics Data System (ADS)

    Racaud, Thierry; Assis-Arantes, Patrick

    1994-12-01

    This paper presents a new approach to the monitoring and control of scientific experiments. This new approach is based on an object-oriented environment composed of three elements: (a) A graphical environment that allows the creation of an object-oriented model of the experiment based on objects, attributes and methods. (b) A language for writing procedures to access the model by sending messages in order to operate the experiment. (c) A man-machine interface based on an interactive graphical layer above the object-oriented representation for controlling and monitoring the experiment. This new approach has been prototyped in a project called "Man-Machine Interface Software for Ground User Terminal", or User Terminal in short. The project is carried out by SPACEBEL Informatique on behalf of the European Space Research and Technology Centre (ESTEC). Although this project has been undertaken for the operation of scientific experiments in space, User Terminal can naturally be used for the monitoring and control of ground based experiments. This article presents the User Terminal system as well as one of the first practical exercises performed in the context of the teleoperation of a liquid science experiment to be shipped into space.

  17. Experience-Based Mitigation of Age-Related Performance Declines: Evidence From Air Traffic Control

    PubMed Central

    Nunes, Ashley; Kramer, Arthur F.

    2010-01-01

    Previous research has found age-related deficits in a variety of cognitive processes. However, some studies have demonstrated age-related sparing on tasks where individuals have substantial experience, often attained over many decades. Here, the authors examined whether decades of experience in a fast-paced demanding profession, air traffic control (ATC), would enable older controllers to perform at high levels of proficiency. The authors also investigated whether older controllers would show diminished age-related decrements on domain-relevant cognitive abilities. Both young and old controllers and noncontrollers performed a battery of cognitive and ATC tasks. Results indicate that although high levels of experience can reduce the magnitude of age-related decline on the component processes that underlie complex task performance, this sparing is limited in scope. More important, however, the authors observed experience-based sparing on simulated ATC tasks, with the sparing being most evident on the more complex air traffic control tasks. These results suggest that given substantial experience, older adults may be quite capable of performing at high levels of proficiency on fast-paced demanding real-world tasks. The implications of these findings for global skilled labor shortages are discussed. PMID:19309213

  18. Abiotic Reductive Immobilization of U(VI) by Biogenic Mackinawite

    SciTech Connect

    Veeramani, Harish; Scheinost, Andreas; Monsegue, Niven; Qafoku, Nikolla; Kukkadapu, Ravi K.; Newville, Mathew; Lanzirotti, Anthony; Pruden, Amy; Murayama, Mitsuhiro; Hochella, Michael F.

    2013-03-01

    During subsurface bioremediation of uranium-contaminated sites, indigenous metal and sulfate-reducing bacteria may utilize a variety of electron acceptors, including ferric iron and sulfate that could lead to the formation of various biogenic minerals in-situ. Sulfides, as well as structural and adsorbed Fe(II) associated with biogenic Fe(II)-sulfide phases, can potentially catalyze abiotic U6+ reduction via direct electron transfer processes. In the present work, the propensity of biogenic mackinawite (Fe1+xS, x = 0 to 0.11) to reduce U6+ abiotically was investigated. The biogenic mackinawite produced by Shewanella putrefaciens strain CN32 was characterized by employing a suite of analytical techniques including TEM, SEM, XAS and Mössbauer analyses. Nanoscale and bulk analyses (microscopic and spectroscopic techniques, respectively) of biogenic mackinawite after exposure to U6+ indicate the formation of nanoparticulate UO2. This study suggests the relevance of Fe(II) and sulfide bearing biogenic minerals in mediating abiotic U6+ reduction, an alternative pathway in addition to direct enzymatic U6+ reduction.

  19. Titania may produce abiotic oxygen atmospheres on habitable exoplanets

    NASA Astrophysics Data System (ADS)

    Narita, Norio; Enomoto, Takafumi; Masaoka, Shigeyuki; Kusakabe, Nobuhiko

    2015-12-01

    The search for habitable exoplanets in the Universe is actively ongoing in the field of astronomy. The biggest future milestone is to determine whether life exists on such habitable exoplanets. In that context, oxygen in the atmosphere has been considered strong evidence for the presence of photosynthetic organisms. In this paper, we show that a previously unconsidered photochemical mechanism by titanium (IV) oxide (titania) can produce abiotic oxygen from liquid water under near ultraviolet (NUV) lights on the surface of exoplanets. Titania works as a photocatalyst to dissociate liquid water in this process. This mechanism offers a different source of a possibility of abiotic oxygen in atmospheres of exoplanets from previously considered photodissociation of water vapor in upper atmospheres by extreme ultraviolet (XUV) light. Our order-of-magnitude estimation shows that possible amounts of oxygen produced by this abiotic mechanism can be comparable with or even more than that in the atmosphere of the current Earth, depending on the amount of active surface area for this mechanism. We conclude that titania may act as a potential source of false signs of life on habitable exoplanets.Reference:Narita N. et al.,Scientific Reports 5, Article number: 13977 (2015)http://www.nature.com/articles/srep13977

  20. Titania may produce abiotic oxygen atmospheres on habitable exoplanets.

    PubMed

    Narita, Norio; Enomoto, Takafumi; Masaoka, Shigeyuki; Kusakabe, Nobuhiko

    2015-09-10

    The search for habitable exoplanets in the Universe is actively ongoing in the field of astronomy. The biggest future milestone is to determine whether life exists on such habitable exoplanets. In that context, oxygen in the atmosphere has been considered strong evidence for the presence of photosynthetic organisms. In this paper, we show that a previously unconsidered photochemical mechanism by titanium (IV) oxide (titania) can produce abiotic oxygen from liquid water under near ultraviolet (NUV) lights on the surface of exoplanets. Titania works as a photocatalyst to dissociate liquid water in this process. This mechanism offers a different source of a possibility of abiotic oxygen in atmospheres of exoplanets from previously considered photodissociation of water vapor in upper atmospheres by extreme ultraviolet (XUV) light. Our order-of-magnitude estimation shows that possible amounts of oxygen produced by this abiotic mechanism can be comparable with or even more than that in the atmosphere of the current Earth, depending on the amount of active surface area for this mechanism. We conclude that titania may act as a potential source of false signs of life on habitable exoplanets.

  1. Abiotic Deposition of Fe Complexes onto Leptothrix Sheaths.

    PubMed

    Kunoh, Tatsuki; Hashimoto, Hideki; McFarlane, Ian R; Hayashi, Naoaki; Suzuki, Tomoko; Taketa, Eisuke; Tamura, Katsunori; Takano, Mikio; El-Naggar, Mohamed Y; Kunoh, Hitoshi; Takada, Jun

    2016-06-03

    Bacteria classified in species of the genus Leptothrix produce extracellular, microtubular, Fe-encrusted sheaths. The encrustation has been previously linked to bacterial Fe oxidases, which oxidize Fe(II) to Fe(III) and/or active groups of bacterial exopolymers within sheaths to attract and bind aqueous-phase inorganics. When L. cholodnii SP-6 cells were cultured in media amended with high Fe(II) concentrations, Fe(III) precipitates visibly formed immediately after addition of Fe(II) to the medium, suggesting prompt abiotic oxidation of Fe(II) to Fe(III). Intriguingly, these precipitates were deposited onto the sheath surface of bacterial cells as the population was actively growing. When Fe(III) was added to the medium, similar precipitates formed in the medium first and were abiotically deposited onto the sheath surfaces. The precipitates in the Fe(II) medium were composed of assemblies of globular, amorphous particles (ca. 50 nm diameter), while those in the Fe(III) medium were composed of large, aggregated particles (≥3 µm diameter) with a similar amorphous structure. These precipitates also adhered to cell-free sheaths. We thus concluded that direct abiotic deposition of Fe complexes onto the sheath surface occurs independently of cellular activity in liquid media containing Fe salts, although it remains unclear how this deposition is associated with the previously proposed mechanisms (oxidation enzyme- and/or active group of organic components-involved) of Fe encrustation of the Leptothrix sheaths.

  2. Abiotic carbonate dissolution traps carbon in a semiarid desert

    PubMed Central

    Fa, Keyu; Liu, Zhen; Zhang, Yuqing; Qin, Shugao; Wu, Bin; Liu, Jiabin

    2016-01-01

    It is generally considered that desert ecosystems release CO2 to the atmosphere, but recent studies in drylands have shown that the soil can absorb CO2 abiotically. However, the mechanisms and exact location of abiotic carbon absorption remain unclear. Here, we used soil sterilization, 13CO2 addition, and detection methods to trace 13C in the soil of the Mu Us Desert, northern China. After 13CO2 addition, a large amount of 13CO2 was absorbed by the sterilised soil, and 13C was found enriched both in the soil gaseous phase and dissolved inorganic carbon (DIC). Further analysis indicated that about 79.45% of the total 13C absorbed by the soil was trapped in DIC, while the amount of 13C in the soil gaseous phase accounted for only 0.22% of the total absorbed 13C. However, about 20.33% of the total absorbed 13C remained undetected. Our results suggest that carbonate dissolution might occur predominately, and the soil liquid phase might trap the majority of abiotically absorbed carbon. It is possible that the trapped carbon in the soil liquid phase leaches into the groundwater; however, further studies are required to support this hypothesis. PMID:27020762

  3. Titania may produce abiotic oxygen atmospheres on habitable exoplanets

    PubMed Central

    Narita, Norio; Enomoto, Takafumi; Masaoka, Shigeyuki; Kusakabe, Nobuhiko

    2015-01-01

    The search for habitable exoplanets in the Universe is actively ongoing in the field of astronomy. The biggest future milestone is to determine whether life exists on such habitable exoplanets. In that context, oxygen in the atmosphere has been considered strong evidence for the presence of photosynthetic organisms. In this paper, we show that a previously unconsidered photochemical mechanism by titanium (IV) oxide (titania) can produce abiotic oxygen from liquid water under near ultraviolet (NUV) lights on the surface of exoplanets. Titania works as a photocatalyst to dissociate liquid water in this process. This mechanism offers a different source of a possibility of abiotic oxygen in atmospheres of exoplanets from previously considered photodissociation of water vapor in upper atmospheres by extreme ultraviolet (XUV) light. Our order-of-magnitude estimation shows that possible amounts of oxygen produced by this abiotic mechanism can be comparable with or even more than that in the atmosphere of the current Earth, depending on the amount of active surface area for this mechanism. We conclude that titania may act as a potential source of false signs of life on habitable exoplanets. PMID:26354078

  4. Cell Wall Metabolism in Response to Abiotic Stress.

    PubMed

    Le Gall, Hyacinthe; Philippe, Florian; Domon, Jean-Marc; Gillet, Françoise; Pelloux, Jérôme; Rayon, Catherine

    2015-02-16

    This review focuses on the responses of the plant cell wall to several abiotic stresses including drought, flooding, heat, cold, salt, heavy metals, light, and air pollutants. The effects of stress on cell wall metabolism are discussed at the physiological (morphogenic), transcriptomic, proteomic and biochemical levels. The analysis of a large set of data shows that the plant response is highly complex. The overall effects of most abiotic stress are often dependent on the plant species, the genotype, the age of the plant, the timing of the stress application, and the intensity of this stress. This shows the difficulty of identifying a common pattern of stress response in cell wall architecture that could enable adaptation and/or resistance to abiotic stress. However, in most cases, two main mechanisms can be highlighted: (i) an increased level in xyloglucan endotransglucosylase/hydrolase (XTH) and expansin proteins, associated with an increase in the degree of rhamnogalacturonan I branching that maintains cell wall plasticity and (ii) an increased cell wall thickening by reinforcement of the secondary wall with hemicellulose and lignin deposition. Taken together, these results show the need to undertake large-scale analyses, using multidisciplinary approaches, to unravel the consequences of stress on the cell wall. This will help identify the key components that could be targeted to improve biomass production under stress conditions.

  5. Abiotic reductive immobilization of U(VI) by biogenic mackinawite.

    PubMed

    Veeramani, Harish; Scheinost, Andreas C; Monsegue, Niven; Qafoku, Nikolla P; Kukkadapu, Ravi; Newville, Matt; Lanzirotti, Antonio; Pruden, Amy; Murayama, Mitsuhiro; Hochella, Michael F

    2013-03-05

    During subsurface bioremediation of uranium-contaminated sites, indigenous metal and sulfate-reducing bacteria may utilize a variety of electron acceptors, including ferric iron and sulfate that could lead to the formation of various biogenic minerals in situ. Sulfides, as well as structural and adsorbed Fe(II) associated with biogenic Fe(II)-sulfide phases, can potentially catalyze abiotic U(VI) reduction via direct electron transfer processes. In the present work, the propensity of biogenic mackinawite (Fe 1+x S, x = 0 to 0.11) to reduce U(VI) abiotically was investigated. The biogenic mackinawite produced by Shewanella putrefaciens strain CN32 was characterized by employing a suite of analytical techniques including TEM, SEM, XAS, and Mössbauer analyses. Nanoscale and bulk analyses (microscopic and spectroscopic techniques, respectively) of biogenic mackinawite after exposure to U(VI) indicate the formation of nanoparticulate UO2. This study suggests the relevance of sulfide-bearing biogenic minerals in mediating abiotic U(VI) reduction, an alternative pathway in addition to direct enzymatic U(VI) reduction.

  6. Abiotic Deposition of Fe Complexes onto Leptothrix Sheaths

    PubMed Central

    Kunoh, Tatsuki; Hashimoto, Hideki; McFarlane, Ian R.; Hayashi, Naoaki; Suzuki, Tomoko; Taketa, Eisuke; Tamura, Katsunori; Takano, Mikio; El-Naggar, Mohamed Y.; Kunoh, Hitoshi; Takada, Jun

    2016-01-01

    Bacteria classified in species of the genus Leptothrix produce extracellular, microtubular, Fe-encrusted sheaths. The encrustation has been previously linked to bacterial Fe oxidases, which oxidize Fe(II) to Fe(III) and/or active groups of bacterial exopolymers within sheaths to attract and bind aqueous-phase inorganics. When L. cholodnii SP-6 cells were cultured in media amended with high Fe(II) concentrations, Fe(III) precipitates visibly formed immediately after addition of Fe(II) to the medium, suggesting prompt abiotic oxidation of Fe(II) to Fe(III). Intriguingly, these precipitates were deposited onto the sheath surface of bacterial cells as the population was actively growing. When Fe(III) was added to the medium, similar precipitates formed in the medium first and were abiotically deposited onto the sheath surfaces. The precipitates in the Fe(II) medium were composed of assemblies of globular, amorphous particles (ca. 50 nm diameter), while those in the Fe(III) medium were composed of large, aggregated particles (≥3 µm diameter) with a similar amorphous structure. These precipitates also adhered to cell-free sheaths. We thus concluded that direct abiotic deposition of Fe complexes onto the sheath surface occurs independently of cellular activity in liquid media containing Fe salts, although it remains unclear how this deposition is associated with the previously proposed mechanisms (oxidation enzyme- and/or active group of organic components-involved) of Fe encrustation of the Leptothrix sheaths. PMID:27271677

  7. Abscisic Acid and Abiotic Stress Tolerance in Crop Plants

    PubMed Central

    Sah, Saroj K.; Reddy, Kambham R.; Li, Jiaxu

    2016-01-01

    Abiotic stress is a primary threat to fulfill the demand of agricultural production to feed the world in coming decades. Plants reduce growth and development process during stress conditions, which ultimately affect the yield. In stress conditions, plants develop various stress mechanism to face the magnitude of stress challenges, although that is not enough to protect them. Therefore, many strategies have been used to produce abiotic stress tolerance crop plants, among them, abscisic acid (ABA) phytohormone engineering could be one of the methods of choice. ABA is an isoprenoid phytohormone, which regulates various physiological processes ranging from stomatal opening to protein storage and provides adaptation to many stresses like drought, salt, and cold stresses. ABA is also called an important messenger that acts as the signaling mediator for regulating the adaptive response of plants to different environmental stress conditions. In this review, we will discuss the role of ABA in response to abiotic stress at the molecular level and ABA signaling. The review also deals with the effect of ABA in respect to gene expression. PMID:27200044

  8. Cell Wall Metabolism in Response to Abiotic Stress

    PubMed Central

    Gall, Hyacinthe Le; Philippe, Florian; Domon, Jean-Marc; Gillet, Françoise; Pelloux, Jérôme; Rayon, Catherine

    2015-01-01

    This review focuses on the responses of the plant cell wall to several abiotic stresses including drought, flooding, heat, cold, salt, heavy metals, light, and air pollutants. The effects of stress on cell wall metabolism are discussed at the physiological (morphogenic), transcriptomic, proteomic and biochemical levels. The analysis of a large set of data shows that the plant response is highly complex. The overall effects of most abiotic stress are often dependent on the plant species, the genotype, the age of the plant, the timing of the stress application, and the intensity of this stress. This shows the difficulty of identifying a common pattern of stress response in cell wall architecture that could enable adaptation and/or resistance to abiotic stress. However, in most cases, two main mechanisms can be highlighted: (i) an increased level in xyloglucan endotransglucosylase/hydrolase (XTH) and expansin proteins, associated with an increase in the degree of rhamnogalacturonan I branching that maintains cell wall plasticity and (ii) an increased cell wall thickening by reinforcement of the secondary wall with hemicellulose and lignin deposition. Taken together, these results show the need to undertake large-scale analyses, using multidisciplinary approaches, to unravel the consequences of stress on the cell wall. This will help identify the key components that could be targeted to improve biomass production under stress conditions. PMID:27135320

  9. Apollo experience report guidance and control systems: Primary guidance, navigation, and control system development

    NASA Technical Reports Server (NTRS)

    Holley, M. D.; Swingle, W. L.; Bachman, S. L.; Leblanc, C. J.; Howard, H. T.; Biggs, H. M.

    1976-01-01

    The primary guidance, navigation, and control systems for both the lunar module and the command module are described. Development of the Apollo primary guidance systems is traced from adaptation of the Polaris Mark II system through evolution from Block I to Block II configurations; the discussion includes design concepts used, test and qualification programs performed, and major problems encountered. The major subsystems (inertial, computer, and optical) are covered. Separate sections on the inertial components (gyroscopes and accelerometers) are presented because these components represent a major contribution to the success of the primary guidance, navigation, and control system.

  10. Technetium Reduction and Permanent Sequestration by Abiotic and Biotic Formation of Low-Solubility Sulfide Mineral Phases

    SciTech Connect

    Tratnyek, Paul G.; Tebo, Bradley M.; Fan, Dimin; Anitori, Roberto; Szecsody, Jim; Jansik, Danielle

    2015-11-14

    One way to minimize the mobility of the TcVII oxyanion pertechnetate (TcO4-) is to effect reduction under sulfidogenic conditions (generated abiotically by Fe0 or biotically) to form TcSx, which is significantly slower to oxidize than TcIVO2. In sediment systems, TcSx and other precipitates may oxidize more slowly due to oxygen diffusion limitations to these low permeability precipitate zones. In addition, the TcO4- reduction rate may be more rapid in the presence of sediment because of additional reductive surface phases. This project aims to provide a fundamental understanding of the feasibility of immobilization of TcO4- as TcSx in the vadose zone or groundwater by application nano zero-valent iron (nZVI), and sulfide or sulfate. Biotic batch experiments have used the sulfate-reducing bacterium (SRB) Desulfotomaculum reducens. The iron sulfide mineral mackinawite was generated under these conditions, while vivianite was formed in nZVI only controls. The sulfide/bacteria-containing system consistently reduced aqueous pertechnetate rapidly (> 95% in the first hour), a rate similar to that for the sulfide-free, nZVI only system. Reduced Tc (aged for 3 months) generated in both SRB/nZVI systems was highly resistant to reoxidation. In reduced samples, Tc was found associated with solid phases containing Fe and S (D. reducens/nZVI) or Fe (nZVI only). Experiments using D. reducens without nZVI provided some additional insights. Firstly, stationary phase cultures were able to slowly reduce pertechnetate. Secondly, addition of pertechnetate at the beginning of cell growth (lag phase) resulted in a faster rate of Tc reduction, possibly indicating a direct (e.g. enzymatic) role for D. reducens in Tc reduction. Abiotic batch experiments were conducted with Na2S as the sulfide source. Pertechnetate reduction was

  11. Apollo experience report. Guidance and control systems: Command and service module stabilization and control system

    NASA Technical Reports Server (NTRS)

    Littleton, O. P.

    1974-01-01

    The concepts, design, development, testing, and flight results of the command and service module stabilization and control system are discussed. The period of time covered was from November 1961 to December 1972. Also included are a functional description of the system, a discussion of the major problems, and recommendations for future programs.

  12. Beam transport experiment with a new kicker control system on the HIRFL

    NASA Astrophysics Data System (ADS)

    Wang, Yan-Yu; Zhou, De-Tai; Luo, Jin-Fu; Zhang, Jian-Chuan; Zhou, Wen-Xiong; Ni, Fa-Fu; Yin, Jun; Yin, Jia; Yuan, You-Jin; Shang-Guan, Jing-Bin

    2016-04-01

    A kicker control system is used for beam extraction and injection between two cooling storage rings (CSRs) at the Heavy Ion Research Facility in Lanzhou (HIRFL). To meet the requirements of special physics experiments, the kicker controller has been upgraded, with a new controller designed based on ARM+DSP+FPGA technology and monolithic circuit architecture, which can achieve a precision time delay of 2.5 ns. In September 2014, the new kicker control system was installed in the kicker field, and the test experiment using the system was completed. In addition, a pre-trigger signal was provided by the controller, which was designed to synchronize the beam diagnostic system and physics experiments. Experimental results indicate that the phenomena of “missed kick” and “inefficient kick” were not observed, and the multichannel trigger signal delay could be adjusted individually for kick power supplies in digitization; thus, the beam transport efficiency was improved compared with that of the original system. The fast extraction and injection experiment was successfully completed based on the new kicker control systems for HIRFL. Supported by National Natural Science Foundation of China (U1232123)

  13. Microarray: gateway to unravel the mystery of abiotic stresses in plants.

    PubMed

    Gul, Ambreen; Ahad, Ammara; Akhtar, Sidra; Ahmad, Zarnab; Rashid, Bushra; Husnain, Tayyab

    2016-04-01

    Environmental factors, such as drought, salinity, extreme temperature, ozone poisoning, metal toxicity etc., significantly affect crops. To study these factors and to design a possible remedy, biological experimental data concerning these crops requires the quantification of gene expression and comparative analyses at high throughput level. Development of microarrays is the platform to study the differential expression profiling of the targeted genes. This technology can be applied to gene expression studies, ranging from individual genes to whole genome level. It is now possible to perform the quantification of the differential expression of genes on a glass slide in a single experiment. This review documents recently published reports on the use of microarrays for the identification of genes in different plant species playing their role in different cellular networks under abiotic stresses. The regulation pattern of differentially-expressed genes, individually or in group form, may help us to study different pathways and functions at the cellular and molecular level. These studies can provide us with a lot of useful information to unravel the mystery of abiotic stresses in important crop plants.

  14. Overexpression of a Pathogenesis-Related Protein 10 Enhances Biotic and Abiotic Stress Tolerance in Rice

    PubMed Central

    Wu, Jingni; Kim, Sang Gon; Kang, Kyu Young; Kim, Ju-Gon; Park, Sang-Ryeol; Gupta, Ravi; Kim, Yong Hwan; Wang, Yiming; Kim, Sun Tae

    2016-01-01

    Pathogenesis-related proteins play multiple roles in plant development and biotic and abiotic stress tolerance. Here, we characterize a rice defense related gene named “jasmonic acid inducible pathogenesis-related class 10” (JIOsPR10) to gain an insight into its functional properties. Semi-quantitative RT-PCR analysis showed up-regulation of JIOsPR10 under salt and drought stress conditions. Constitutive over-expression JIOsPR10 in rice promoted shoot and root development in transgenic plants, however, their productivity was unaltered. Further experiments exhibited that the transgenic plants showed reduced susceptibility to rice blast fungus, and enhanced salt and drought stress tolerance as compared to the wild type. A comparative proteomic profiling of wild type and transgenic plants showed that overexpression of JIOsPR10 led to the differential modulation of several proteins mainly related with oxidative stresses, carbohydrate metabolism, and plant defense. Taken together, our findings suggest that JIOsPR10 plays important roles in biotic and abiotic stresses tolerance probably by activation of stress related proteins. PMID:27904462

  15. Connecting RNA Processing to Abiotic Environmental Response in Arabidopsis: the role of a polyadenylation factor

    NASA Astrophysics Data System (ADS)

    Li, Q. Q.; Xu, R.; Hunt, A. G.; Falcone, D. L.

    Plants are constantly challenged by numerous environmental stresses both biotic and abiotic It is clear that plants have evolved to counter these stresses using all but limited means We recently discovered the potential role of a messenger RNA processing factor namely the Arabidopsis cleavage and polyadenylation specificity factor 30 kDa subunit AtCPSF30 when a mutant deficient in this factor displayed altered responses to an array of abiotic stresses This AtCPSF30 mutant named oxt6 exhibited an elevated tolerance to oxidative stress Microarray experiments of oxt6 and its complemented lines revealed an altered gene expression profile among which were antioxidative defense genes Interestingly the same gene encoding AtCPSF30 can also be transcribed into a large transcript that codes for a potential splicing factor Both protein products have a domain for RNA binding and a calmodulin binding domain activities of which have been confirmed by biochemical assays Surprisingly binding of AtCPSF30 to calmodulin inhibits the RNA-binding activity of the protein Mutational analysis shows that a small part of the protein is responsible for calmodulin binding and point mutations in this region abolished both RNA binding activity and the inhibition of this activity by calmodulin Analyses of the potential splicing factor are on going and the results will be presented The interesting possibilities for both the interplay between splicing and polyadenylation and the regulation of these processes by stimuli that act through

  16. Abiotic degradation of methyl parathion by manganese dioxide: Kinetics and transformation pathway.

    PubMed

    Liao, Xiaoping; Zhang, Caixiang; Liu, Yuan; Luo, Yinwen; Wu, Sisi; Yuan, Songhu; Zhu, Zhenli

    2016-05-01

    Methyl parathion, a widely used insecticide around the world, has aroused gradually extensive concern of researchers due to its degradation product such as methyl paraoxon, with higher toxicity for mammals and more recalcitrant. Given the ubiquity of manganese dioxide (MnO2) in soils and aquatic sediments, the abiotic degradation of methyl parathion by α-MnO2 was investigated in batch experiments. It was found that methyl parathion was decomposed up to 90% by α-MnO2 in 30 h and the removal efficiency of methyl parathion depended strongly on the loading of α-MnO2 and pH value in the solution where the reactions followed pseudo-first-order model well. The coexisting metal ions (such as Ca(2+), Mg(2+) and Mn(2+)) weakened markedly the degradation of methyl parathion by α-MnO2. However, the effect of dissolved organic matter (HA-Na) on reaction rates presented two sides: to improve hydrolysis rate but deteriorate oxidation rate of methyl parathion. Based on the degradation products identified by gas chromatography-mass spectrometer (GC/MS) and liquid chromatography high-resolution mass spectrometer (LC/HRMS), both hydrolysis and oxidation processes were proposed to be two predominant reaction mechanisms contributing to methyl parathion degradation by α-MnO2. This study provided meaningful information to elucidate the abiotic dissipation of methyl parathion by manganese oxide minerals in the environment.

  17. A system for conducting igneous petrology experiments under controlled redox conditions in reduced gravity

    NASA Technical Reports Server (NTRS)

    Williams, R. J.

    1986-01-01

    The Space Shuttle and the planned Space Station will permit experimentation under conditions of reduced gravitational acceleration offering experimental petrologists the opportunity to study crystal growth, element distribution, and phase chemistry. In particular the confounding effects of macro and micro scale buoyancy-induced convection and crystal settling or floatation can be greatly reduced over those observed in experiments in the terrestrial laboratory. Also, for experiments in which detailed replication of the environment is important, the access to reduced gravity will permit a more complete simulation of processes that may have occurred on asteroids or in free space. A technique that was developed to control, measure, and manipulate oxygen fugacites with small quantities of gas which are recirculated over the sample is described. This system should be adaptable to reduced gravity space experiments requiring redox control. Experiments done conventionally and those done using this technique yield identical results done in a 1-g field.

  18. Stratospheric controlled perturbation experiment: a small-scale experiment to improve understanding of the risks of solar geoengineering.

    PubMed

    Dykema, John A; Keith, David W; Anderson, James G; Weisenstein, Debra

    2014-12-28

    Although solar radiation management (SRM) through stratospheric aerosol methods has the potential to mitigate impacts of climate change, our current knowledge of stratospheric processes suggests that these methods may entail significant risks. In addition to the risks associated with current knowledge, the possibility of 'unknown unknowns' exists that could significantly alter the risk assessment relative to our current understanding. While laboratory experimentation can improve the current state of knowledge and atmospheric models can assess large-scale climate response, they cannot capture possible unknown chemistry or represent the full range of interactive atmospheric chemical physics. Small-scale, in situ experimentation under well-regulated circumstances can begin to remove some of these uncertainties. This experiment-provisionally titled the stratospheric controlled perturbation experiment-is under development and will only proceed with transparent and predominantly governmental funding and independent risk assessment. We describe the scientific and technical foundation for performing, under external oversight, small-scale experiments to quantify the risks posed by SRM to activation of halogen species and subsequent erosion of stratospheric ozone. The paper's scope includes selection of the measurement platform, relevant aspects of stratospheric meteorology, operational considerations and instrument design and engineering.

  19. In vivo experiments of a surgical robot with vision field control for Single Port Endoscopic Surgery.

    PubMed

    Sekiguchi, Yuta; Kobayashi, Yo; Watanabe, Hiroki; Tomono, Yu; Noguchi, Takehiko; Takahashi, Yu; Toyoda, Kazutaka; Uemura, Munenori; Ieiri, Satoshi; Ohdaira, Takeshi; Tomikawa, Morimasa; Hashizume, Makoto; Fujie, Masakatsu G

    2011-01-01

    Recently, robotics systems are focused to assist in Single Port Endoscopic Surgery (SPS). However, the existing system required a manual operation of vision and viewpoint, hindering the surgical task. We proposed a surgical endoscopic robot for SPS with dynamic vision control, the endoscopic view being manipulated by a master controller. The prototype robot consists of a manipulator for vision control, and dual tool tissue manipulators (gripping: 5DOFs, cautery: 3DOFs) can be attached at the tip of sheath manipulator. In particular, this paper focuses on an in vivo experiment. We showed that vision control in the stomach and a cautery task by a cautery tool could be effectively achieved.

  20. Preliminary experiments on active control of fan noise from a turbofan engine

    NASA Technical Reports Server (NTRS)

    Thomas, R. H.; Burdisso, R. A.; Fuller, C. R.; O'Brien, W. F.

    1993-01-01

    In the preliminary experiments reported here, active acoustic sources positioned around the circumference of a turbofan engine were used to control the fan noise radiated forward through the inlet. The main objective was to demonstrate the potential of active techniques to alleviate the noise pollution that will be produced by the next generation of larger engines. A reduction of up to 19 dB in the radiation directivity was demonstrated in a zone that encompasses a 30-deg angle, near the error sensor, while spillover effects were observed toward the lateral direction. The simultaneous control of two tones was also demonstrated using two identical controllers in a parallel control configuration.

  1. Applications of small computers for systems control on the Tandem Mirror Experiment-Upgrade

    SciTech Connect

    Bork, R.G.; Kane, R.J.; Moore, T.L.

    1983-11-29

    Desktop computers operating into a CAMAC-based interface are used to control and monitor the operation of the various subsystems on the Tandem Mirror Experiment-Upgrade (TMX-U) at Lawrence Livermore National Laboratory (LLNL). These systems include: shot sequencer/master timing, neutral beam control (four consoles), magnet power system control, ion-cyclotron resonant heating (ICRH) control, thermocouple monitoring, getter system control, gas fueling system control, and electron-cyclotron resonant heating (ECRH) monitoring. Two additional computers are used to control the TMX-U neutral beam test stand and provide computer-aided repair/test and development of CAMAC modules. These machines are usually programmed in BASIC, but some codes have been interpreted into assembly language to increase speed. Details of the computer interfaces and system complexity are described as well as the evolution of the systems to their present states.

  2. Carbon Isotopes of Alkanes in Hydrothermal Abiotic Organic Synthesis Processes at High Temperatures and Pressures: An Experimental Study

    NASA Technical Reports Server (NTRS)

    Fu, Qi; Socki, Richard A.; Niles, Paul B.

    2010-01-01

    Observation of methane in the Martian atmosphere has been reported by different detection techniques [1-4]. With more evidence showing extensive water-rock interaction in Martian history [5-7], abiotic formation by Fischer-Tropsch Type (FTT) synthesis during serpentization reactions may be one possible process responsible for methane generation on Mars [8, 9]. While the experimental studies performed to date leave little doubt that chemical reactions exist for the abiotic synthesis of organic compounds by mineral surface-catalyzed reactions [10-12], little is known about the reaction pathways by which CO2 and/or CO are reduced under hydrothermal conditions. Carbon and hydrogen isotope measurements of alkanes have been used as an effective tool to constrain the origin and reaction pathways of hydrocarbon formation. Alkanes generated by thermal breakdown of high molecular weight organic compounds have carbon and hydrogen isotopic signatures completely distinct from those formed abiotically [13-15]. Recent experimental studies, however, showed that different abiogenic hydrocarbon formation processes (e.g., polymerization vs. depolymerization) may have different carbon and hydrogen isotopic patterns [16]. Results from previous experiments studying decomposition of higher molecular weight organic compounds (lignite) also suggested that pressure could be a crucial factor affecting fractionation of carbon isotopes [17]. Under high pressure conditions, no experimental data are available describing fractionation of carbon isotope during mineral catalyzed FTT synthesis. Thus, hydrothermal experiments present an excellent opportunity to provide the requisite carbon isotope data. Such data can also be used to identify reaction pathways of abiotic organic synthesis under experimental conditions.

  3. Response of plant tundra communities to changes in abiotic and biotic environments: Importance of the temporal dimension

    NASA Astrophysics Data System (ADS)

    Saccone, P.; Virtanen, R.

    2015-12-01

    Understanding of ecosystem response to changing environment have been improved by the convergence of observational and experimental approaches that allow disentangling mechanisms involved and large scale subsequent patterns. However, such approaches often face context-dependence of underlying processes, and a major challenge of community ecology is to deepen our understanding of this context-dependency for reliable upscaling. Here we used the results from several transplant experiments of heath communities in the Northern Fennoscandian during the last two decades to investigate the relative importance of abiotic and biotic drivers and the plant functional response. The plant community composition of blocks of heath vegetation from diverse origins transplanted in contrasted abiotic and biotic conditions was monitored from 6 to 23 years depending on the design. Considering both abiotic severity and biotic environment, the transplantation along altitudinal gradient constituted major habitat perturbation, in particular for communities from the mountain tundra vulnerable to strong functional shift. In addition, the joint effects of multiple drivers associated to grazing pressure and abiotic micro heterogeneity resulted in divergent community in the long-term. However, the different factors operated on different temporal scales. The vegetation depending on their origin and functional type also showed contrasted patterns from immediate and transient response to strong biological inertia. Our results reveal the potential for alternative response of plant communities depending on the interplay between the multiple drivers and the functional attributes of the vegetation. This interplay should drive plant communities toward divergent alternative states, but our ability to extrapolate longer-term trajectories from partial dynamics is challenged by the temporal differences in drivers pressure and plant response. The responses to manipulation appear as successional processes and

  4. Unusual materials effects observed on the thermal control surfaces experiment (S0069)

    NASA Technical Reports Server (NTRS)

    Zwiener, James M.; Wilkes, Donald R.; Hummer, Leigh L.

    1991-01-01

    A number of unusual effects were observed on the Thermal Control Surface Experiment (TCSE) test samples, front cover, and structural components. These include induced UV fluorescence, the migration and degradation of KRS-5 materials, atomic oxygen effects, contamination, texturing, discoloration, and meteoroid/debris impact. LDEF (Long Duration Exposure Facility) mission induced fluorescence was observed on several TCSE samples. Similar fluorescence was observed on LDEF leading edge materials from Experiment A0114.

  5. Design of a CO2 laser power control system for a Spacelab microgravity experiment

    NASA Technical Reports Server (NTRS)

    Wenzler, Carl J.; Eichenberg, Dennis J.

    1990-01-01

    The surface tension driven convection experiment (STDCE) is a Space Transportation System flight experiment manifested to fly aboard the USML-1 Spacelab mission. A CO2 laser is used to heat a spot on the surface of silicone oil contained inside a test chamber. Several CO2 laser control systems were evaluated and the selected system will be interfaced with the balance of the experimental hardware to constitute a working engineering model. Descriptions and a discussion of these various design approaches are presented.

  6. Design of a Synthetic Aperture Array to Support Experiments in Active Control of Scattering

    DTIC Science & Technology

    1990-06-01

    IIC FILE COPY DESIGN OF A SYNTHETIC APERTURE ARRAY TO SUPPORT EXPERIMENTS IN ACTIVE CONTROL OF SCATTERING by JAMES P. DULLEA B.N.E. GEORGIA...Ain Sonin Clmairnnn, Mechancal Engineering Departmenlal Graduate Committee 90 09 24 053 DESIGN OF A SYNTHETIC APERTURE ARRAY TO SUPPORT EXPERIMENTS IN...partial fulfillment of the requirements for the Degrees of Naval Engineer and Master of Science in Mechanical Engineering Abstract A synthetic aperture

  7. Spatial variability of biotic and abiotic tree establishment constraints across a treeline ecotone in the Alaska Range

    USGS Publications Warehouse

    Stueve, K.M.; Isaacs, R.E.; Tyrrell, L.E.; Densmore, R.V.

    2011-01-01

    Throughout interior Alaska (USA), a gradual warming trend in mean monthly temperatures occurred over the last few decades (;2-48C). The accompanying increases in woody vegetation at many alpine treeline (hereafter treeline) locations provided an opportunity to examine how biotic and abiotic local site conditions interact to control tree establishment patterns during warming. We devised a landscape ecological approach to investigate these relationships at an undisturbed treeline in the Alaska Range. We identified treeline changes between 1953 (aerial photography) and 2005 (satellite imagery) in a geographic information system (GIS) and linked them with corresponding local site conditions derived from digital terrain data, ancillary climate data, and distance to 1953 trees. Logistic regressions enabled us to rank the importance of local site conditions in controlling tree establishment. We discovered a spatial transition in the importance of tree establishment controls. The biotic variable (proximity to 1953 trees) was the most important tree establishment predictor below the upper tree limit, providing evidence of response lags with the abiotic setting and suggesting that tree establishment is rarely in equilibrium with the physical environment or responding directly to warming. Elevation and winter sun exposure were important predictors of tree establishment at the upper tree limit, but proximity to trees persisted as an important tertiary predictor, indicating that tree establishment may achieve equilibrium with the physical environment. However, even here, influences from the biotic variable may obscure unequivocal correlations with the abiotic setting (including temperature). Future treeline expansion will likely be patchy and challenging to predict without considering the spatial variability of influences from biotic and abiotic local site conditions. ?? 2011 by the Ecological Society of America.

  8. Initial Experiments on Fuzzy Control for Nuclear Reactor Operations at the Belgian Reactor 1

    SciTech Connect

    Da Ruan

    2003-08-15

    The application of fuzzy logic control (FLC) in the domain of the nuclear industry presents a tremendous challenge. The main reason for this is the public awareness of the risks of nuclear reactors and the very strict safety regulations in force for nuclear power plants. The very same regulations prevent a researcher from quickly introducing novel control methods into this field. On the other hand, the application of FLC has, despite the ominous sound of the word 'fuzzy' to nuclear engineers, a number of very desirable advantages over classical control, e.g., its robustness and the capability to include human experience into the controller. In this paper an FLC for controlling the power level of a nuclear reactor is described. The study is intended to assess the applicability of FLC in this domain. The final goal is to develop an optimized and intrinsically safe controller. After reviewing some available literature on FLC in nuclear reactors, an FLC is proposed and first tested by comparing it with the classical controller of the Belgian reactor 1 (BR1). In the next step the BR1 at the Belgian Nuclear Research Center (SCK-CEN) was used as a test bed to implement a programmable logic controller-based hardware controller. The BR1 reactor is internationally regarded as a nuclear calibration reference. It therefore provides an excellent environment for this type of experiment because over the years considerable knowledge of the static and dynamic properties of the reactor has been accumulated. The project (1995-1999) aimed at investigating the added value and technical limits of FLC for nuclear reactor operations. The progress made in these experiments including closed-loop experiments are presented and discussed in this paper.

  9. Design and test of a mechanically pumped two-phase thermal control flight experiment

    NASA Technical Reports Server (NTRS)

    Grote, M. G.; Stark, J. A.; Butler, C. D.; Mcintosh, R.

    1987-01-01

    A flight experiment of a mechanically pumped two-phase ammonia thermal control system, incorporating a number of new component designs, has been assembled and tested in a 1-g environment. Additional microgravity tests are planned on the Space Shuttle when Shuttle flights are resumed. The primary purpose of this experiment is to evaluate the operation of a mechanically pumped two-phase ammonia system, with emphasis on determining the performance of an evaporative Two-Phase Mounting Plate. The experiment also evaluates the performance of other specially designed components, such as the two-phase reservoir for temperature control, condensing radiator/heat sink, spiral tube boiler, and pressure drop experiment. The 1-g tests have shown that start-up of the two-phase experiment is easily accomplished with only a partial fill of ammonia. The experiment maintained a constant mounting plate temperature without flow rate controls over a very wide range of heat loads, flow rates, inlet flow conditions and exit qualities. The tests also showed the successful operation of the mounting plate in the heat sharing condensing mode.

  10. Simulation and experiment research on the proportional pressure control of water-assisted injection molding

    NASA Astrophysics Data System (ADS)

    Zhou, Hua; Chen, Yinglong; Zhang, Zengmeng; Yang, Huayong

    2012-05-01

    Water-assisted injection molding (WAIM), a newly developed fluid-assisted injection molding technology has drawn more and more attentions for the energy saving, short cooling circle time and high quality of products. Existing research for the process of WAIM has shown that the pressure control of the injecting water is mostly important for the WAIM. However, the proportional pressure control for the WAIM system is quite complex due to the existence of nonlinearities in the water hydraulic system. In order to achieve better pressure control performance of the injecting water to meet the requirements of the WAIM, the proportional pressure control of the WAIM system is investigated both numerically and experimentally. A newly designed water hydraulic system for WAIM is first modeled in AMEsim environment, the load characteristics and the nonlinearities of water hydraulic system are both considered, then the main factors affecting the injecting pressure and load flow rate are extensively studied. Meanwhile, an open-loop model-based compensation control strategy is employed to regulate the water injection pressure and a feedback proportional integrator controller is further adopted to achieve better control performance. In order to verify the AMEsim simulation results WAIM experiment for particular Acrylonitrile Butadiene Styrene (ABS) parts is implemented and the measured experimental data including injecting pressure and flow rate results are compared with the simulation. The good coincidence between experiment and simulation shows that the AMEsim model is accurate, and the tracking performance of the load pressure indicates that the proposed control strategy is effective for the proportional pressure control of the nonlinear WAIM system. The proposed proportional pressure control strategy and the conclusions drawn from simulation and experiment contribute to the application of water hydraulic proportional control and WAIM technology.

  11. Optimized feedback control system modeling of resistive wall modes for burning plasmas experiments

    NASA Astrophysics Data System (ADS)

    Katsuro-Hopkins, Oksana Nikolaevna

    A numerical study of active feedback control system performance and optimization for tokamak Resistive Wall Modes (RWM) is the subject of this thesis. The ability to accurately model and predict the performance of an active MHD control systems is critical to present and future advanced confinement scenarios and fusion reactor design studies. The computer code VALEN has been designed to calculate the performance of a MHD feedback control system in an arbitrary geometry. The simulation of realistic effects in feedback systems, such as noise, time delays and filters is of particular importance. In this work realistic measurement noise analysis was added to VALEN and used to design the RWM feedback control amplifier power level for the DIII-D experiment. Modern control theory based on a state-space formulation obtained from VALEN was applied to design an Optimal Controller and Observer based on a reduced VALEN model. A quantitative low order model of the VALEN state space was derived from the high dimensional intrinsic state space structure of the VALEN using methods of a balanced realization and matched DC gain truncation. These techniques for the design of an optimal controller and optimal observer were applied to models of the DIII-D and ITER experiments and showed an order of magnitude reduction of the required control coil current and voltage in the presence of white noise as compared to a traditional, classical PID controller. This optimal controller for the ITER burning plasma experiment was robust from the no-wall pressure limit to a pressure value well above those achieved with a classical PID controller and could approach the ideal wall limit.

  12. Epigenetic control of mobile DNA as an interface between experience and genome change

    PubMed Central

    Shapiro, James A.

    2014-01-01

    Mobile DNA in the genome is subject to RNA-targeted epigenetic control. This control regulates the activity of transposons, retrotransposons and genomic proviruses. Many different life history experiences alter the activities of mobile DNA and the expression of genetic loci regulated by nearby insertions. The same experiences induce alterations in epigenetic formatting and lead to trans-generational modifications of genome expression and stability. These observations lead to the hypothesis that epigenetic formatting directed by non-coding RNA provides a molecular interface between life history events and genome alteration. PMID:24795749

  13. Control Circuits for a Rocket Payload Neutralization Experiment and Other Topics

    DTIC Science & Technology

    1974-10-01

    AD-A008 039 CONTROL CIRCUITS FOR A ROCKET PAYLOAD NEUTRALIZATION EXPERIMENT AND OTHER TOPICS Raimundas Sukys , et al Northeastern University...tJ 113104 AFCRL-TR-74-0580 CONTROL CIRCUITS FOR A ROCKET PAYLOAD NEUTRALIZATION EXPERIMENT AND OTHER TOPICS Raimundas Sukys Steven Goldberg...O2R ;nAN. NUMULR4I)V Raimiundas Sukys Steven Goldberg Fl 9628-74-C-0042 9. PLRFOAMINGORNGANIZATION NAME AND ADD’RESS 10 PROEGPAM (LL.ENT.PflOJErT

  14. Operational experience of SST1 NBI control system with prototype Ion source

    NASA Astrophysics Data System (ADS)

    Patel, V. B.; Patel, P. J.; Singh, N. P.; Patel, G. B.; Onali, Raja; Tripathi, V.; Thakkar, D.; Gupta, L. N.; Prahlad, V.; Sharma, S. K.; Bandyopadyay, M.; Chakraborty, A. K.; Baruah, U. K.; Mattoo, S. K.; Nbi Team

    2010-02-01

    This paper presents operational experience of integrated control of the arc-filament and High-voltage power supply of Steady State Tokamak (SST)-1 NBI system using Versa Module Europa (VME) system on prototype Ion source. The control algorithm is implemented on the VxWorks operating system using 'C' language. This paper also describes the operating sequence and controls on power supply system. Discharge and Filament power supplies are controlled in such a way so that necessary discharge current can be available in Ion Source. The discharge current is controlled by manipulating the filament current. Close loop control is implemented on each filament power supply with feedback from Discharge Current to control the overall discharge inside the ion source. Necessary actions for shut OFF and subsequent Turn ON are also taken during breakdowns between the Grids of the ion source. Total numbers of breakdowns are also monitored. Shot is terminated, if the breakdown count is higher than the set value. This control system can be programmed to restart High-voltage power supply within 5mS after breakdown occurs. This control system is capable to handle the all types of dynamics in the system. This paper also presents results of experiment.

  15. An on-orbit experiment for dynamics and control of large structures

    NASA Technical Reports Server (NTRS)

    Buchanan, H. J.; Schock, R. W.; Waites, H. B.

    1984-01-01

    Attention is given to the definition of the on-orbit dynamic testing that is currently being planned for the flight of a large solar array test article, the Solar Array Flight Experiment (SAFE 1), which consists of a coilable longeron mast that deploys a large solar array blanket. Also discussed is the design of an additional experiment employing this structure in conjunction with a two- or three-axis gimbal system, in order to demonstrate control techniques applicable to such large structures. SAFE 1 experiment objectives, hardware, software, and the experimental operations foreseen are discussed.

  16. LabVIEW-based control and data acquisition system for cathodoluminescence experiments.

    PubMed

    Bok, J; Schauer, P

    2011-11-01

    Computer automation of cathodoluminescence (CL) experiments using equipment developed in our laboratory is described. The equipment provides various experiments for CL efficiency, CL spectra, and CL time response studies. The automation was realized utilizing the graphical programming environment LabVIEW. The developed application software with procedures for equipment control and data acquisition during various CL experiments is presented. As the measured CL data are distorted by technical limitations of the equipment, such as equipment spectral sensitivity and time response, data correction algorithms were incorporated into the procedures. Some examples of measured data corrections are presented.

  17. Viewing artworks: contributions of cognitive control and perceptual facilitation to aesthetic experience.

    PubMed

    Cupchik, Gerald C; Vartanian, Oshin; Crawley, Adrian; Mikulis, David J

    2009-06-01

    When we view visual images in everyday life, our perception is oriented toward object identification. In contrast, when viewing visual images as artworks, we also tend to experience subjective reactions to their stylistic and structural properties. This experiment sought to determine how cognitive control and perceptual facilitation contribute to aesthetic perception along with the experience of emotion. Using functional MRI, we show that aesthetic perception activated bilateral insula which we attribute to the experience of emotion. Moreover, while adopting the aesthetic orientation activated the left lateral prefrontal cortex, paintings that facilitated visuospatial exploration activated the left superior parietal lobule. The results suggest that aesthetic experience is a function of the interaction between top-down orienting of attention and bottom-up perceptual facilitation.

  18. Seeing conflict and engaging control: Experience with contrastive language benefits executive function in preschoolers.

    PubMed

    Doebel, Sabine; Zelazo, Philip David

    2016-12-01

    Engaging executive function often requires overriding a prepotent response in favor of a conflicting but adaptive one. Language may play a key role in this ability by supporting integrated representations of conflicting rules. We tested whether experience with contrastive language that could support such representations benefits executive function in 3-year-old children. Children who received brief experience with language highlighting contrast between objects, attributes, and actions showed greater executive function on two of three 'conflict' executive function tasks than children who received experience with contrasting stimuli only and children who read storybooks with the experimenter, controlling for baseline executive function. Experience with contrasting stimuli did not benefit executive function relative to reading books with the experimenter, indicating experience with contrastive language, rather than experience with contrast generally, was key. Experience with contrastive language also boosted spontaneous attention to contrast, consistent with improvements in representing contrast. These findings indicate a role for language in executive function that is consistent with the Cognitive Complexity and Control theory's key claim that coordinating conflicting rules is critical to overcoming perseveration, and suggest new ideas for testing theories of executive function.

  19. Importance of temperature control for HEFLEX, a biological experiment for Spacelab 1. [plant gravitational physiology study

    NASA Technical Reports Server (NTRS)

    Chapman, D. K.; Brown, A. H.

    1979-01-01

    The importance of temperature control to HEFLEX, a Spacelab experiment designed to measure kinetic properties of Helianthis nutation in a low-g environment, is discussed. It is argued that the development of the HEFLEX experiment has been severely hampered by the inadequate control of ambient air temperature provided by the spacecraft module design. A worst case calculation shows that delivery of only 69% of the maximum yield of useful data from the HEFLEX system is guaranteed; significant data losses from inadequate temperature control are expected. The magnitude of the expected data losses indicates that the cost reductions associated with imprecise temperature controls may prove to be a false economy in the long term.

  20. Modular system for data acquisition and control of experiments with digital output

    NASA Astrophysics Data System (ADS)

    Calabria, Mauro F.; Deza, Roberto R.

    2010-11-01

    In the present work, the design of an efficient, modular, and scalable data acquisition and control system is described. It consists of an array of microcontrollers and memories, which feed a single concentrating unit whose information can be accessed by means of a universal series bus (USB) interface to be processed later on. Signal levels can be controlled through a set of digital potentiometers. This system is ideal for experiments with a large number of digital outputs.

  1. Big brother - a fully automated control system for the DELPHI experiment

    SciTech Connect

    Franek, B.; Sekulin, R.; Doenszelmann, M.; Gasper, C.; Charpentier, Jonker, M.

    1994-12-31

    The integrated control system of the DELPHI experiment is described. It allows the data to be taken in an almost automatic fashion. This is achieved by computer monitoring of the states of the LEP machine, the DELPHI subdetectors (SC) and the Data Acquisition System (DAS). Depending on these states, computer initiated actions are then taken. It has been designed using {open_quote}State Manager{close_quote} concept already used for local and central controls of DAS and SC.

  2. Anti-inflammatory and antioxidative activity of anthocyanins from purple basil leaves induced by selected abiotic elicitors.

    PubMed

    Szymanowska, Urszula; Złotek, Urszula; Karaś, Monika; Baraniak, Barbara

    2015-04-01

    This paper investigates changes in the anti-inflammatory and antioxidative activity of anthocyanins from purple basil (Ocimum basilicum L.) leaves induced by arachidonic acid (AA), jasmonic acid (JA) and β-aminobutyric acid (BABA). The anthocyanins content was significantly increased by all elicitors used in this study; however, no increase was observed in the antioxidant activity of the analyzed extracts. Additionally, a significant decrease by about 50% in the ability to chelate Fe(II) was noted. Further, an increase in the potential anti-inflammatory activity of basil anthocyanins was observed after treatment with each the abiotic elicitor. The IC50 value for lipoxygenase inhibition was almost twice as low after elicitation as that of the control. Also, cyclooxygenase inhibition by anthocyanins was stimulated by abiotic elicitors, except for JA-sample. Additionally, HPLC-analysis indicated that elicitation with AA, JA and BABA caused increases in content most of all anthocyanin compounds.

  3. Effect of abiotic factors on seasonal population dynamics of Lutzomyia longipalpis (Diptera: Psychodidae) in northeastern Brazil.

    PubMed

    Ximenes, Maria de Fátima Freire de Melo; Castellón, Eloy G; De Souza, Maria de Fátima; Menezes, Alexandre A Lara; Queiroz, José Wilton; Macedo e Silva, Virgínia Penéllope; Jerônimo, Selma M B

    2006-09-01

    The resurgence of visceral leishmaniasis in Brazil increases the need for studies to elucidate the spatial and temporal dynamics of Lutzomyia longipalpis (Lutz and Neiva) (Diptera: Psychodidae), the vector of Leishmania infantum, the causative agent of visceral leishmaniasis in Brazil. Sand flies were captured in peridomestic habitats biweekly for 3 yr. Cross-correlation tests and spectral analysis were used to analyze the simultaneous and lag-time correlations between Lu. longipalpis population densities and abiotic factors of temperature, relative humidity, wind velocity, and rainfall. Distinct seasonal patterns were observed for males and females, with intervals of 6 mo between population peaks for males and 12 mo for females. Peak female population densities lagged 3 mo behind the maximum annual temperature. Female population density was negatively correlated with relative humidity. An increase in average wind velocity was followed by a decrease in the number of females for 2 wk. Understanding the relationship between the seasonal population dynamics of Lu. longipalpis and abiotic factors will contribute to the design of better control measures to decrease transmission of L. infantum and consequently the incidence of leishmaniasis.

  4. Non-coding RNAs in the plant response to abiotic stress.

    PubMed

    Contreras-Cubas, Cecilia; Palomar, Miguel; Arteaga-Vázquez, Mario; Reyes, José Luis; Covarrubias, Alejandra A

    2012-10-01

    As sessile organisms, plants have to cope with the ever-changing environment as well as with numerous forms of stress. To react to these external cues, plants have evolved a suite of response mechanisms operating at many different levels, ranging from physiological to molecular processes that provide the organism with a wide phenotypic plasticity, allowing for fine tuning of the reactions to these adverse circumstances. During the past decade, non-coding RNAs (ncRNAs) have emerged as key regulatory molecules, which contribute to a significant portion of the transcriptome in eukaryotes and are involved in the control of transcriptional and post-transcriptional gene regulatory pathways. Although accumulated evidence supports an important role for ncRNAs in plant response and adaptation to abiotic stress, their mechanism(s) of action still remains obscure and a functional characterization of the ncRNA repertoire in plants is still needed. Moreover, common features in the biogenesis of different small ncRNAs, and in some cases, cross talk between different gene regulatory pathways may add to the complexity of these pathways and could play important roles in modulating stress responses. Here we review the various ncRNAs that have been reported to participate in the response to abiotic stress in plants, focusing on their importance in plant adaptation and evolution. Understanding how ncRNAs work may reveal novel mechanisms involved in the plant responses to the environment.

  5. Transgenic Alfalfa Plants Expressing the Sweetpotato Orange Gene Exhibit Enhanced Abiotic Stress Tolerance

    PubMed Central

    Wang, Zhi; Ke, Qingbo; Kim, Myoung Duck; Kim, Sun Ha; Ji, Chang Yoon; Jeong, Jae Cheol; Lee, Haeng-Soon; Park, Woo Sung; Ahn, Mi-Jeong; Li, Hongbing; Xu, Bingcheng; Deng, Xiping; Lee, Sang-Hoon; Lim, Yong Pyo; Kwak, Sang-Soo

    2015-01-01

    Alfalfa (Medicago sativa L.), a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. We recently demonstrated that the sweetpotato Orange gene (IbOr) is involved in increasing carotenoid accumulation and enhancing resistance to multiple abiotic stresses. In this study, in an effort to improve the nutritional quality and environmental stress tolerance of alfalfa, we transferred the IbOr gene into alfalfa (cv. Xinjiang Daye) under the control of an oxidative stress-inducible peroxidase (SWPA2) promoter through Agrobacterium tumefaciens-mediated transformation. Among the 11 transgenic alfalfa lines (referred to as SOR plants), three lines (SOR2, SOR3, and SOR8) selected based on their IbOr transcript levels were examined for their tolerance to methyl viologen (MV)-induced oxidative stress in a leaf disc assay. The SOR plants exhibited less damage in response to MV-mediated oxidative stress and salt stress than non-transgenic plants. The SOR plants also exhibited enhanced tolerance to drought stress, along with higher total carotenoid levels. The results suggest that SOR alfalfa plants would be useful as forage crops with improved nutritional value and increased tolerance to multiple abiotic stresses, which would enhance the development of sustainable agriculture on marginal lands. PMID:25946429

  6. Hypothesis/review: contribution of putrescine to 4-aminobutyrate (GABA) production in response to abiotic stress.

    PubMed

    Shelp, Barry J; Bozzo, Gale G; Trobacher, Christopher P; Zarei, Adel; Deyman, Kristen L; Brikis, Carolyne J

    2012-09-01

    4-Aminobutyrate (GABA) accumulates in various plant parts, including bulky fruits such as apples, in response to abiotic stress. It is generally believed that the GABA is derived from glutamate, although a contribution from polyamines is possible. Putrescine, but not spermidine and spermine, generally accumulates in response to the genetic manipulation of polyamine biosynthetic enzymes and abiotic stress. However, the GABA levels in stressed plants are influenced by processes other than putrescine availability. It is hypothesized that the catabolism of putrescine to GABA is regulated by a combination of gene-dependent and -independent processes. The expression of several putative diamine oxidase genes is weak, but highly stress-inducible in certain tissues of Arabidopsis. In contrast, candidate genes that encode 4-aminobutyraldehyde dehydrogenase are highly constitutive, but not stress inducible. Changes in O(2) availability and cellular redox balance due to stress may directly influence the activities of diamine oxidase and 4-aminobutyraldehyde dehydrogenase, thereby restricting GABA formation. Apple fruit is known to accumulate GABA under controlled atmosphere storage and therefore could serve as a model system for investigating the relative contribution of putrescine and glutamate to GABA production.

  7. Real-time Equilibrium Reconstruction and Isoflux Control of Plasma Shape and Position in the National Spherical Torus Experiment (NSTX)

    SciTech Connect

    D. Mueller; D.A. Gates; J.E. Menard; J.R. Ferron; S.A. Sabbagh

    2004-08-11

    The implementation of the rtEFIT-isoflux algorithm in the digital control system for NSTX has led to improved ability to control the plasma shape. In particular, it has been essential for good gap control for radio-frequency experiments, for control of drsep in H-mode studies, and for X-point height control and {kappa} control in a variety of experiments.

  8. Controlling factors of Ca isotope fractionation in scleractinian corals evaluated by temperature, pH and light controlled culture experiments

    NASA Astrophysics Data System (ADS)

    Inoue, Mayuri; Gussone, Nikolaus; Koga, Yasuko; Iwase, Akihiro; Suzuki, Atsushi; Sakai, Kazuhiko; Kawahata, Hodaka

    2015-10-01

    In this study, the 44Ca/40Ca ratios of Porites australiensis grown under three different culture experiments composed of temperature, pH and light controlled culture experiments are measured. The temperature dependent isotope fractionation of 0.02‰/°C deduced from this study is similar to inorganic aragonite, but the degree of isotope fractionation is about +0.4‰ offset in corals. These observations agree with earlier results on different coral species, suggesting Ca isotope fractionation during Ca transmembrane transport in corals. While in cultured corals a significant temperature dependence of δ44Ca is observed, the relationships between calcium isotope fractionation and pH as well as light intensity are negligible. Therefore variation of δ44Ca in Porites corals is mainly controlled by temperature. A combination of δ44Ca and Sr/Ca of corals in temperature controlled experiments cannot be explained by Rayleigh type fractionation directly from a fluid, which is seawater-like in terms of δ44Ca and Sr/Ca. Through coral-specific biomineralization processes, overall mean δ44Ca of scleractinian corals including previous studies are different from biogenic aragonites secreted by sclerosponges and pteropods, but are comparable with those of bivalves as well as calcitic coccolithophores and foraminifers. These findings are important for better understanding biomineralization in corals and in order to constrain the Ca isotopic composition of oceanic Ca sinks in response to climate changes and associated with shifts of calcite and aragonite seas.

  9. Experiments of Closed-Loop Flow Control for Laminar Boundary Layers

    NASA Astrophysics Data System (ADS)

    Zhang, Zhenyu

    The Tollmien-Schlichting (T-S) instability is important for the transition process of boundary layer flows. The cancellation of its unstable modes will help delay the occurrence of transition and then turbulence. In this article the T-S instability was attempted to attenuate through an active closed-loop control strategy. The frequency responses of unstable travelling T-S waves were obtained for the implementation of control strategy. Information such as velocity disturbances of both incoming unstable waves and cancelling waves were then fed into the control system. A controller was deliberately designed and tested for the active cancellation of instabilities which were excited by velocity disturbances normal to the wall. The cancellation performance of the control system was validated by experiments in a low turbulence wind tunnel, which proved the functionality of above control strategy upon the active cancellation of boundary layer instabilities.

  10. Experiments in advanced control concepts for space robotics - An overview of the Stanford Aerospace Robotics Laboratory

    NASA Technical Reports Server (NTRS)

    Hollars, M. G.; Cannon, R. H., Jr.; Alexander, H. L.; Morse, D. F.

    1987-01-01

    The Stanford University Aerospace Robotics Laboratory is actively developing and experimentally testing advanced robot control strategies for space robotic applications. Early experiments focused on control of very lightweight one-link manipulators and other flexible structures. The results are being extended to position and force control of mini-manipulators attached to flexible manipulators and multilink manipulators with flexible drive trains. Experimental results show that end-point sensing and careful dynamic modeling or adaptive control are key to the success of these control strategies. Free-flying space robot simulators that operate on an air cushion table have been built to test control strategies in which the dynamics of the base of the robot and the payload are important.

  11. Resilience of Penicillium resedanum LK6 and exogenous gibberellin in improving Capsicum annuum growth under abiotic stresses.

    PubMed

    Khan, Abdul Latif; Waqas, Muhammad; Lee, In-Jung

    2015-03-01

    Understanding how endophytic fungi mitigate abiotic stresses in plants will be important in a changing global climate. A few endophytes can produce phytohormones, but their ability to induce physiological changes in host plants during extreme environmental conditions are largely unexplored. In the present study, we investigated the ability of Penicillium resedanum LK6 to produce gibberellins and its role in improving the growth of Capsicum annuum L. under salinity, drought, and heat stresses. These effects were compared with exogenous application of gibberellic acid (GA3). Endophyte treatment significantly increased shoot length, biomass, chlorophyll content, and the photosynthesis rate compared with the uninfected control during abiotic stresses. The endophyte and combined endophyte + GA3 treatments significantly ameliorated the negative effects of stresses compared with the control. Stress-responsive endogenous abscisic acid and its encoding genes, such as zeaxanthin epoxidase, 9-cis-epoxycarotenoid dioxygenase 3, and ABA aldehyde oxidase 3, were significantly reduced in endophyte-treated plants under stress. Conversely, salicylic acid and biosynthesis-related gene (isochorismate synthase) had constitutive expressions while pathogenesis related (PR1 and PR5) genes showed attenuated responses during endophyte treatment under abiotic stresses. The present findings suggest that endophytes have effects comparable to those of exogenous GA3; both can significantly increase plant growth and yield under changing environmental conditions by reprogramming the host plant's physiological responses.

  12. A low cost PCI-VME controller for control and data acquisition systems on fusion experiments

    NASA Astrophysics Data System (ADS)

    Carvalho, B. B.; Sousa, J.; Varandas, C. A. F.

    1999-01-01

    A universal hardware interface system that allows the transparent use of digital instrumentation of different buses has been developed using the VMEbus as the host platform. In this article a new controller for the VME system, based on a desktop-type PCI Pentium motherboard and a locally developed PCI-VME interconnector is described. This interconnector is composed of two boards, which are connected by an ac-terminated flexible cable and inserted into slots of the PCI motherboard and VME crate. This system decreases the cost of the global system and permits low priced and modular upgradeability. The software drivers have been developed for a UNIX environment using the LINUX-LAB project in a user-friendly approach.

  13. Fuzzy-Logic Based Vibration Suppression Control Experiments on Active Structures

    NASA Astrophysics Data System (ADS)

    Kwak, M. K.; Sciulli, D.

    1996-03-01

    This paper is concerned with the fuzzy-logic based vibration suppression control of active structures equipped with piezoelectric sensors and actuators. The control methodology is based on the fuzzy logic control of the variable structures system type. The sufficient condition for the closed-loop stability of the decentralized fuzzy control for the system equipped with collocated sensors and actuators is derived from the sufficient condition of the decentralized collocated variable system control. Hence, it is concluded that the fuzzy control is in fact the variation of the variable structure system control in this case. Comparison of the variable structure system to the fuzzy control leads to a new fuzzy rule of the vibration suppression of the active structure equipped with collocated sensors and actuators. It is shown that the fuzzy-logic control can be designed for the collocated system without any knowledge of the system to be controlled. However, this may not be true in the case of multi-input and multi-output non-collocated systems. All the developments are demonstrated by means of a real-time fuzzy control experiment on the cantilever beam with surface-bonded piezoceramic sensors and actuators.

  14. Vortex breakdown and control experiments in the Ames-Dryden water tunnel

    NASA Technical Reports Server (NTRS)

    Owen, F. K.; Peake, D. J.

    1986-01-01

    Flow-field measurements have been made to determine the effects of core blowing on vortex breakdown and control. The results of these proof-of-concept experiments clearly demonstrate the usefulness of water tunnels as test platforms for advanced flow-field simulation and measurement.

  15. An e-Learning System with MR for Experiments Involving Circuit Construction to Control a Robot

    ERIC Educational Resources Information Center

    Takemura, Atsushi

    2016-01-01

    This paper proposes a novel e-Learning system for technological experiments involving electronic circuit-construction and controlling robot motion that are necessary in the field of technology. The proposed system performs automated recognition of circuit images transmitted from individual learners and automatically supplies the learner with…

  16. The Dynamic Nature of Leisure Experience: An Application of Affect Control Theory.

    ERIC Educational Resources Information Center

    Lee, BongKoo; Shafer, C. Scott

    2002-01-01

    Applied Affect Control Theory (ACT) to investigate the interaction process between leisure participants and their environment. Surveys of people on an urban, multiple-use trail indicated that most exhibited a dynamic emotional experience even though they were in the setting a short time. Respondents exhibited different emotions across events.…

  17. Displaying Special Characters and Symbols in Computer-Controlled Reaction Time Experiments.

    ERIC Educational Resources Information Center

    Friel, Brian M.; Kennison, Shelia M.

    A procedure for using MEL2 (Version 2.0 of Microcomputer Experimental Laboratory) and FontWINDOW to present special characters and symbols in computer-controlled reaction time experiments is described. The procedure permits more convenience and flexibility than in tachistocopic and projection techniques. FontWINDOW allows researchers to design…

  18. Randomized Controlled Trial of Teaching Methods: Do Classroom Experiments Improve Economic Education in High Schools?

    ERIC Educational Resources Information Center

    Eisenkopf, Gerald; Sulser, Pascal A.

    2016-01-01

    The authors present results from a comprehensive field experiment at Swiss high schools in which they compare the effectiveness of teaching methods in economics. They randomly assigned classes into an experimental and a conventional teaching group, or a control group that received no specific instruction. Both teaching treatments improve economic…

  19. Ear Playing and Aural Development in the Instrumental Lesson: Results from a "Case-Control" Experiment

    ERIC Educational Resources Information Center

    Baker, David; Green, Lucy

    2013-01-01

    This article reports on a case-control experiment that was conducted in 2012 as part of the Ear Playing Project (EPP) at the Institute of Education, University of London. The EPP developed from the "informal learning" strand of Musical Futures and engaged instrumental students in the UK in learning from specially-created audio recordings…

  20. Water-deficiency effects on single leaf gas exchange and on C4 pathway enzymes of maize genotypes with differing abiotic stress tolerance

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Responses to drought were studied using two maize inbred lines (B76 and B106) and a commercial maize hybrid (Zea mays L. cv. Silver Queen) with differing resistance to abiotic stress. Maize seedlings were grown in pots in controlled environment chambers for 17 days and watering was withheld from on...

  1. A system for conducting igneous petrology experiments under controlled redox conditions in reduced gravity

    NASA Technical Reports Server (NTRS)

    Williams, Richard J.

    1987-01-01

    The Space Shuttle and the planned Space Station will permit experimentation under conditions of reduced gravitational acceleration offering experimental petrologists the opportunity to study crystal growth, element distribution, and phase chemistry. In particular the confounding effects of macro and micro scale buoyancy-induced convection and crystal settling or flotation can be greatly reduced over those observed in experiments in the terrestrial laboratory. Also, for experiments in which detailed replication of the environment is important, the access to reduced gravity will permit a more complete simulation of processes that may have occurred on asteroids or in free space. A technique that was developed to control, measure, and manipulate oxygen fugacities with small quantities of gas which are recirculated over the sample. This system could be adaptable to reduced gravity space experiments requiring redox control.

  2. A disturbance isolation controller for the solar electric propulsion system flight experiment

    NASA Technical Reports Server (NTRS)

    Waites, H. B.

    1982-01-01

    A disturbance isolation controller (DIC) is developed for a simplified model of the solar electric propulsion system (SEPS) flight experiment which consists of a rigid Sperry gimbal torquer (AGS) mounted to a rigid orbiter and the SEPS solar array (rigid) end mounted to the AGS. The main purpose of the DIC is to reduce the effects of orbiter disturbances which are transmitted to the flight experiment. The DIC uses an observer, which does not require the direct measurement of the plant inputs, to obtain estimates of the plant states and the rate of the plant states. The state and rate of state information is used to design a controller which isolates disturbances from specified segments of the plant, and for the flight experiment, the isolated segment is the SEPS solar array.

  3. Wind tunnel experiments to prove a hydraulic passive torque control concept for variable speed wind turbines

    NASA Astrophysics Data System (ADS)

    Diepeveen, N. F. B.; Jarquin-Laguna, A.

    2014-12-01

    In this paper the results are presented of experiments to prove an innovative concept for passive torque control of variable speed wind turbines using fluid power technology. It is demonstrated that by correctly configuring the hydraulic drive train, the wind turbine rotor operates at or near maximum aerodynamic efficiency for below rated wind speeds. The experiments with a small horizontal-axis wind turbine rotor, coupled to a hydraulic circuit, were conducted at the Open Jet Facility of the Delft University of Technology. In theory, the placement of a nozzle at the end of the hydraulic circuit causes the pressure and hence the rotor torque to increase quadratically with flow speed and hence rotation speed. The rotor torque is limited by a pressure relief valve. Results from the experiments proved the functionality of this passive speed control concept. By selecting the correct nozzle outlet area the rotor operates at or near the optimum tip speed ratio.

  4. Flow and quiet eye: the role of attentional control in flow experience.

    PubMed

    Harris, David J; Vine, Samuel J; Wilson, Mark R

    2017-02-25

    This report was designed to investigate the role of effective attention control in flow states, by developing an experimental approach to the study of flow. A challenge-skill balance manipulation was applied to self-paced netball and basketball shooting tasks, with point of gaze recorded through mobile eye tracking. Quiet eye was used to index optimal control of visual attention. While the experimental manipulation was found to have no effect, quiet eye was associated with the experience of flow. Furthermore, mediation revealed an indirect effect of quiet eye on performance through flow experience. This study provides initial evidence that flow may be preceded by changes in visual attention, suggesting that further investigation of visual attention may elucidate the cognitive mechanisms behind flow experience.

  5. Experiments on reduction of propeller induced interior noise by active control of cylinder vibration

    NASA Technical Reports Server (NTRS)

    Fuller, C. R.; Jones, J. D.

    1987-01-01

    The feasibility of reducing interior noise caused by advanced turbo propellers by controlling the vibration of aircraft fuselages was investigated by performing experiments in an anechoic chamber with an aircraft model test rig and apparatus. It was found that active vibration control provides reasonable global attenuation of interior noise levels for the cases of resonant (at 576 Hz) and forced (at 708 Hz) system response. The controlling mechanism behind the effect is structural-acoustic coupling between the shell and the contained field, termed interface modal filtering.

  6. HyPRP1 Gene Suppressed by Multiple Stresses Plays a Negative Role in Abiotic Stress Tolerance in Tomato

    PubMed Central

    Li, Jinhua; Ouyang, Bo; Wang, Taotao; Luo, Zhidan; Yang, Changxian; Li, Hanxia; Sima, Wei; Zhang, Junhong; Ye, Zhibiao

    2016-01-01

    Many hybrid proline-rich protein (HyPRP) genes respond to biotic and abiotic stresses in plants, but little is known about their roles other than as putative cell-wall structural proteins. A HyPRP1 gene encodes a protein with proline-rich domain, and an eight-cysteine motif was identified from our previous microarray experiments on drought-tolerant tomato. In this study, the expression of the HyPRP1 gene in tomato was suppressed under various abiotic stresses, such as drought, high salinity, cold, heat, and oxidative stress. Transgenic functional analysis showed no obvious changes in phenotypes, but enhanced tolerance to various abiotic stresses (e.g., oxidative stress, dehydration, and salinity) was observed in RNAi transgenic plants. Interestingly, several SO2 detoxification-related enzymes, including sulfite oxidase, ferredoxins (Fds), and methionine sulfoxide reductase A (Msr A), were revealed in HyPRP1-interacting proteins identified by Yeast Two-Hybrid screening. More sulfates and transcripts of Msr A and Fds were accumulated in HyPRP1 knockdown lines when wild-type plants were exposed to SO2 gas. Our findings illustrate that the tomato HyPRP1 is a negative regulator of salt and oxidative stresses and is probably involved in sulfite metabolism. PMID:27446190

  7. Distinguishing Biotic from Abiotic Phosphate Oxygen Isotopic Signatures

    NASA Astrophysics Data System (ADS)

    Blake, R.; Moyer, C.; Colman, A.; Liang, Y.; Dogru, D.

    2006-05-01

    On earth, phosphate has a strong biological oxygen isotope signature due to its concentration and intense cycling by living organisms as an essential nutrient. Phosphate does not undergo oxygen isotope exchange with water at low temperature without enzymatic catalysis, making the oxygen isotope ratio (18O/16O) of phosphate, δ18OP, an attractive biosignature in the search for early and extraterrestrial life. Recent laboratory and field studies have demonstrated that the δ18OP value of dissolved inorganic phosphate (PO4) records specific microbial activity and enzymatic reaction pathways in both laboratory cultures and natural waters/sediments (Blake et al., 2005; Colman et al 2005; Liang and Blake, 2005). Phosphate oxygen isotope biosignatures may be distinguished from abiotic signatures by: (1) evaluating the degree of temperature-dependent PO4-water oxygen isotope exchange in aqueous systems and deviation from equilibrium; and (2) evolution from an abiotic P reservoir signature towards a biotic P reservoir signature. Important abiotic processes potentially affecting phosphate δ18OP values include dissolution/precipitation, adsorption/desorption, recrystallization of PO4 mineral phases, diagenesis and metamorphism. For most of these processes, the recording, retention and alteration of δ18OP biosignatures have not been evaluated. Deep-sea hydrothermal vent fields are an ideal system in which to study the preservation and alteration of δ18OP biosignatures, as well as potential look-alikes produced by heat-promoted PO4 -water oxygen isotope exchange. Results from recent studies of δ18OP biosignatures in hydrothermal deposits near 9 and 21 degrees N. EPR and at Loihi seamount will be presented.

  8. Pathways for abiotic organic synthesis at submarine hydrothermal fields

    PubMed Central

    McDermott, Jill M.; Seewald, Jeffrey S.; German, Christopher R.; Sylva, Sean P.

    2015-01-01

    Arguments for an abiotic origin of low-molecular weight organic compounds in deep-sea hot springs are compelling owing to implications for the sustenance of deep biosphere microbial communities and their potential role in the origin of life. Theory predicts that warm H2-rich fluids, like those emanating from serpentinizing hydrothermal systems, create a favorable thermodynamic drive for the abiotic generation of organic compounds from inorganic precursors. Here, we constrain two distinct reaction pathways for abiotic organic synthesis in the natural environment at the Von Damm hydrothermal field and delineate spatially where inorganic carbon is converted into bioavailable reduced carbon. We reveal that carbon transformation reactions in a single system can progress over hours, days, and up to thousands of years. Previous studies have suggested that CH4 and higher hydrocarbons in ultramafic hydrothermal systems were dependent on H2 generation during active serpentinization. Rather, our results indicate that CH4 found in vent fluids is formed in H2-rich fluid inclusions, and higher n-alkanes may likely be derived from the same source. This finding implies that, in contrast with current paradigms, these compounds may form independently of actively circulating serpentinizing fluids in ultramafic-influenced systems. Conversely, widespread production of formate by ΣCO2 reduction at Von Damm occurs rapidly during shallow subsurface mixing of the same fluids, which may support anaerobic methanogenesis. Our finding of abiogenic formate in deep-sea hot springs has significant implications for microbial life strategies in the present-day deep biosphere as well as early life on Earth and beyond. PMID:26056279

  9. Pathways for abiotic organic synthesis at submarine hydrothermal fields.

    PubMed

    McDermott, Jill M; Seewald, Jeffrey S; German, Christopher R; Sylva, Sean P

    2015-06-23

    Arguments for an abiotic origin of low-molecular weight organic compounds in deep-sea hot springs are compelling owing to implications for the sustenance of deep biosphere microbial communities and their potential role in the origin of life. Theory predicts that warm H2-rich fluids, like those emanating from serpentinizing hydrothermal systems, create a favorable thermodynamic drive for the abiotic generation of organic compounds from inorganic precursors. Here, we constrain two distinct reaction pathways for abiotic organic synthesis in the natural environment at the Von Damm hydrothermal field and delineate spatially where inorganic carbon is converted into bioavailable reduced carbon. We reveal that carbon transformation reactions in a single system can progress over hours, days, and up to thousands of years. Previous studies have suggested that CH4 and higher hydrocarbons in ultramafic hydrothermal systems were dependent on H2 generation during active serpentinization. Rather, our results indicate that CH4 found in vent fluids is formed in H2-rich fluid inclusions, and higher n-alkanes may likely be derived from the same source. This finding implies that, in contrast with current paradigms, these compounds may form independently of actively circulating serpentinizing fluids in ultramafic-influenced systems. Conversely, widespread production of formate by ΣCO2 reduction at Von Damm occurs rapidly during shallow subsurface mixing of the same fluids, which may support anaerobic methanogenesis. Our finding of abiogenic formate in deep-sea hot springs has significant implications for microbial life strategies in the present-day deep biosphere as well as early life on Earth and beyond.

  10. Design of a high-lift experiment in water including active flow control

    NASA Astrophysics Data System (ADS)

    Beutel, T.; Sattler, S.; El Sayed, Y.; Schwerter, M.; Zander, M.; Büttgenbach, S.; Leester-Schädel, M.; Radespiel, R.; Sinapius, M.; Wierach, P.

    2014-07-01

    This paper describes the structural design of an active flow-control experiment. The aim of the experiment is to investigate the increase in efficiency of an internally blown Coanda flap using unsteady blowing. The system uses tailor-made microelectromechanical (MEMS) pressure sensors to determine the state of the oncoming flow and an actuated lip to regulate the mass flow and velocity of a stream near a wall over the internally blown flap. Sensors and actuators are integrated into a highly loaded system that is extremely compact. The sensors are connected to a bus system that feeds the data into a real-time control system. The piezoelectric actuators using the d 33 effect at a comparable low voltage of 120 V are integrated into a lip that controls the blowout slot height. The system is designed for closed-loop control that efficiently avoids flow separation on the Coanda flap. The setup is designed for water-tunnel experiments in order to reduce the free-stream velocity and the system’s control frequency by a factor of 10 compared with that in air. This paper outlines the function and verification of the system’s main components and their development.

  11. MHD control experiments in the Extrap T2R Reversed Field Pinch

    NASA Astrophysics Data System (ADS)

    Marrelli, L.; Bolzonella, T.; Brunsell, P.; Cecconello, M.; Drake, J.; Franz, P.; Gregoratto, D.; Manduchi, G.; Martin, P.; Ortolani, S.; Paccagnella, R.; Piovesan, P.; Spizzo, G.; Yadikin, D.; Zanca, P.

    2004-11-01

    We report here on MHD active control experiments performed in the Extrap T2R device, which has been recently equipped with a set of 32 feedback controlled saddle coils couples. Experiments aiming at selectively exciting a resonant resistive instability in order to actively induce Quasi Single Helicity states will be presented. Open loop experiments have in fact shown that a spectrum with one dominant mode can be excited in a high aspect ratio device like T2R. In addition, evidences of controlled braking of tearing modes, which spontaneously rotate in T2R, have been gathered, allowing the determination of a threshold for mode wall locking. Different feedback control schemes have been implemented. In particular, mode suppression schemes proved successful in delaying resistive wall modes growth and in increasing the discharge duration: this suggests a hybrid mode control scenario, in which RWM are suppressed and QSH is induced. Radiation imaging and internal magnetic field reconstructions performed with the ORBIT code will be presented.

  12. Design of the EO-1 Pulsed Plasma Thruster Attitude Control Experiment

    NASA Technical Reports Server (NTRS)

    Zakrzwski, Charles; Sanneman, Paul; Hunt, Teresa; Blackman, Kathie; Bauer, Frank H. (Technical Monitor)

    2001-01-01

    The Pulsed Plasma Thruster (PPT) Experiment on the Earth Observing 1 (EO-1) spacecraft has been designed to demonstrate the capability of a new generation PPT to perform spacecraft attitude control. The PPT is a small, self-contained pulsed electromagnetic Propulsion system capable of delivering high specific impulse (900-1200 s), very small impulse bits (10-1000 micro N-s) at low average power (less than 1 to 100 W). EO-1 has a single PPT that can produce torque in either the positive or negative pitch direction. For the PPT in-flight experiment, the pitch reaction wheel will be replaced by the PPT during nominal EO-1 nadir pointing. A PPT specific proportional-integral-derivative (PID) control algorithm was developed for the experiment. High fidelity simulations of the spacecraft attitude control capability using the PPT were conducted. The simulations, which showed PPT control performance within acceptable mission limits, will be used as the benchmark for on-orbit performance. The flight validation will demonstrate the ability of the PPT to provide precision pointing resolution. response and stability as an attitude control actuator.

  13. Abiotic gas formation drives nitrogen loss from a desert ecosystem.

    PubMed

    McCalley, Carmody K; Sparks, Jed P

    2009-11-06

    In arid environments such as deserts, nitrogen is often the most limiting nutrient for biological activity. The majority of the ecosystem nitrogen flux is typically thought to be driven by production and loss of reactive nitrogen species by microorganisms in the soil. We found that high soil-surface temperatures (greater than 50 degrees C), driven by solar radiation, are the primary cause of nitrogen loss in Mojave Desert soils. This abiotic pathway not only enables the balancing of arid ecosystem nitrogen budgets, but also changes our view of global nitrogen cycling and the predicted impact of climate change and increased temperatures on nitrogen bioavailability.

  14. Arms control and nonproliferation technologies: The non-proliferation experiment. First quarter 1994

    SciTech Connect

    Staehle, G.; Stull, S.; Talaber, C.

    1994-05-01

    In this issue of Arms Control and Nonproliferation Technologies we present the initial findings of the recent Non-Proliferation Experiment (NPE), conducted by the Department of Energy at the Nevada Test Site. Through an introduction and pictorial walk-through, Marv Denny and Jay Zucca of Lawrence Livermore National Laboratory describe the overall experiment. This is followed by scientific and technical abstracts of the complex suite of experiments and analyses, which were presented at the Symposium on Non-Proliferation Experiment Results and Implications for Test Ban Treaties, April 19--21, 1994. Questions regarding the ongoing analysis and conclusions from the NPE should be directed to Leslie Casey in the Office of Research and Development within the Office of Nonproliferation and National Security of DOE. Her phone number is 202-586-2151.

  15. International Space Station Attitude Control and Energy Storage Experiment: Effects of Flywheel Torque

    NASA Technical Reports Server (NTRS)

    Roithmayr, Carlos M.

    1999-01-01

    The Attitude Control and Energy Storage Experiment is currently under development for the International Space Station; two counter-rotating flywheels will be levitated with magnetic bearings and placed in vacuum housings. The primary objective of the experiment is to store and discharge energy, in combination with existing batteries, into the electrical power system. The secondary objective is to use the flywheels to exert torque on the Station; a simple torque profile has been designed so that the Station's Control Moment Gyroscopes will be assisted in maintaining torque equilibrium attitude. Two energy storage contingencies could result in the inadvertent application of torque by the flywheels to the Station: an emergency shutdown of one flywheel rotor while the other remains spinning, and energy storage with only one rotor instead of the counterrotating pair. Analysis of these two contingencies shows that attitude control and the microgravity environment will not be adversely affected.

  16. Precision Pointing Control System (PPCS) system design and analysis. [for gimbaled experiment platforms

    NASA Technical Reports Server (NTRS)

    Frew, A. M.; Eisenhut, D. F.; Farrenkopf, R. L.; Gates, R. F.; Iwens, R. P.; Kirby, D. K.; Mann, R. J.; Spencer, D. J.; Tsou, H. S.; Zaremba, J. G.

    1972-01-01

    The precision pointing control system (PPCS) is an integrated system for precision attitude determination and orientation of gimbaled experiment platforms. The PPCS concept configures the system to perform orientation of up to six independent gimbaled experiment platforms to design goal accuracy of 0.001 degrees, and to operate in conjunction with a three-axis stabilized earth-oriented spacecraft in orbits ranging from low altitude (200-2500 n.m., sun synchronous) to 24 hour geosynchronous, with a design goal life of 3 to 5 years. The system comprises two complementary functions: (1) attitude determination where the attitude of a defined set of body-fixed reference axes is determined relative to a known set of reference axes fixed in inertial space; and (2) pointing control where gimbal orientation is controlled, open-loop (without use of payload error/feedback) with respect to a defined set of body-fixed reference axes to produce pointing to a desired target.

  17. Soldier experiments and assessments using SPEAR speech control system for UGVs

    NASA Astrophysics Data System (ADS)

    Brown, Jonathan; Blanco, Chris; Czerniak, Jeffrey; Hoffman, Brian; Hoffman, Orin; Juneja, Amit; Ngia, Lester; Pruthi, Tarun; Liu, Dongqing

    2010-04-01

    This paper reports on a Soldier Experiment performed by the Army Research Lab's Human Research Engineering Directorate (HRED) Field Element located at the Maneuver Center of Excellence, Ft. Benning, and a Limited Use Assessment conducted by the Marine Corps Forces Pacific Command Experimentation Center (MEC) at Camp Pendleton evaluating the effectiveness of using speech commands to control an Unmanned Ground Vehicle. SPEAR, developed by Think-A-Move, Ltd., provides speech control of UGVs. SPEAR detects user speech in the ear canal with an earpiece containing an in-ear microphone. The system design provides up to 30 dB of passive noise reduction, enabling it to work well in high-noise environments, where traditional speech systems, using external microphones, fail; it also utilizes a proprietary speech recognition engine. SPEAR has been integrated with iRobot's PackBot 510 with FasTac Kit, and with Multi-Robot Operator Control Unit (MOCU), developed by SPAWAR Systems Center Pacific. These integrated systems allow speech to supplement the hand-controller for multi-modal control of different UGV functions simultaneously. HRED's experiment measured the impact of SPEAR on reducing the cognitive load placed on UGV Operators and the time to complete specific tasks. Army NCOs and Officer School Candidates participated in this experiment, which found that speech control was faster than manual control to complete tasks requiring menu navigation, as well as reducing the cognitive load on UGV Operators. The MEC assessment examined speech commands used for two different missions: Route Clearance and Cordon and Search; participants included Explosive Ordnance Disposal Technicians and Combat Engineers. The majority of the Marines thought it was easier to complete the mission scenarios with SPEAR than with only using manual controls, and that using SPEAR improved their situational awareness. Overall results of these Assessments are reported in the paper, along with possible

  18. Development of Control Teaching Material for Mechatronics Education Based on Experience

    NASA Astrophysics Data System (ADS)

    Tasaki, Takao; Watanabe, Shinichi; Shikanai, Yoshihito; Ozaki, Koichi

    In this paper, we have developed a teaching material for technical high school students to understand the control technique. The material makes the students understanding the control technique through the sensibility obtained from the experience of riding the robot. We have considered the correspondence of the teaching material with the ARCS Model. Therefore, the material aims to improve the interest and the willingness to learn mechatronics and control technique by experiencing the difference of the response by the change in the control parameters. As the results of the questionnaire to the technical high school students in the class, we have verified educative effect of the teaching material which can be improved willingness of learning and interesting for mechatronics and control technique.

  19. Initial experiments on the end-point control of a flexible one-link robot

    NASA Technical Reports Server (NTRS)

    Cannon, R. H., Jr.; Schmitz, E.

    1984-01-01

    The present investigation is concerned with initial experiments regarding a specific unsolved control problem which appeared to be central to advances in the art of robotics. This problem involves the control of a flexible member (one link of a robot system). The position of the end-effector, called the end point or tip, is controlled by measuring that position and using the measurement as a basis for applying control torque to the other end of the flexible member, as for instance, the robot's elbow joint. A description is presented of the features of the first experimental arm which has been made, and an outline is provided of the general strategy for controlling it using its tip sensor and shoulder torquer.

  20. Plasma Shape Control on the National Spherical Torus Experiment (NSTX) using Real-time Equilibrium Reconstruction

    SciTech Connect

    D.A. Gates; J.R. Ferron; M. Bell; T. Gibney; R. Johnson; R.J. Marsala; D. Mastrovito; J.E. Menard; D. Mueller; B. Penaflor; S.A. Sabbagh; T. Stevenson

    2005-04-15

    Plasma shape control using real-time equilibrium reconstruction has been implemented on the National Spherical Torus Experiment (NSTX). The rtEFIT code originally developed for use on DIII-D was adapted for use on NSTX. The real-time equilibria provide calculations of the flux at points on the plasma boundary, which is used as input to a shape control algorithm known as isoflux control. The flux at the desired boundary location is compared to a reference flux value, and this flux error is used as the basic feedback quantity for the poloidal-field coils on NSTX. The hardware that comprises the control system is described, as well as the software infrastructure. Examples of precise boundary control are also presented.