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Sample records for academy press nap

  1. Cockpit napping

    NASA Technical Reports Server (NTRS)

    Graeber, R. Curtis; Rosekind, Mark R.; Connell, Linda J.; Dinges, David F.

    1990-01-01

    The results of a NASA-sponsored study examining the effectiveness of a brief, preplanned cockpit rest period to improve pilot alertness and performance in nonaugmented long-haul flight operations are discussed. Four regularly scheduled trans-Pacific flight legs were studied. The shortest flight legs were about 7 h and the longest about 9.5 h, with duty periods averaging about 11 h and layovers about 25 h. Three-person B747 crews were divided randomly into two volunteer pilot groups. These crews were nonaugmented, and therefore no relief pilots were available. The rest group, consisting of four crews, was allowed a 40 min opportunity to rest during the overwater cruise portion of the flight. On a preplanned, rotating basis, individual crew members were allowed to nap. It is concluded that a preplanned cockpit nap is associated with significantly better behavioral performance and higher levels of physiological alertness and that this can be accomplished without disrupting normal flight operations or compromising safety.

  2. Naps (For Parents)

    MedlinePlus

    ... age-by-age guidelines give an idea of average daily sleep requirements: Birth to 6 months: Infants require about ... Toddlers generally require 12 to 14 hours of sleep, including an afternoon nap ... (3 to 5 years): Preschoolers average about 11 to 12 hours at night, plus ...

  3. NAP alpha-aminoisobutyric acid (IsoNAP).

    PubMed

    Gozes, Illana; Schirer, Yulie; Idan-Feldman, Anat; David, Merav; Furman-Assaf, Sharon

    2014-01-01

    We set out to identify NAP (davunetide) analogs, providing neuroprotection and reducing tau pathology, specifically addressing protection against protein misfolding. NAP (NAPVSIPQ, intranasal formulation AL-108) is a drug candidate that (1) had a statistically significant impact on two measures, namely digit span and delayed-match-to-sample, tests of verbal recall and visual working memory, respectively, in patient population of mild cognitive impairment [preceding Alzheimer's disease (AD)] and (2) protected functional activities of daily living in schizophrenia patients. Previous preclinical studies have shown that stabilization of NAP by replacement of all L-amino acids by D-amino acids resulted in an active peptide, D-NAP. Other NAP mimetics are now explored. A new NAP analog was designed that included replacement of the proline residues by alpha-aminoisobutyric acid to enhance β-sheet breaker characteristics, thereby reducing protein misfolding. Three lines of investigations were chosen: (1) protection against the AD-associated amyloid β (1-42), Aβ1-42, peptide toxicity in cell cultures; (2) inhibition of AD-associated tau aggregation in vitro; and (3) cognitive protection in a mouse model of deficiencies of the NAP parent protein, activity-dependent neuroprotective protein (ADNP), exhibiting tau pathology and neurodegeneration. NAP alpha-aminoisobutyric acid (IsoNAP) protected neurons against AD-associated Aβ1-42-toxicity, inhibited the aggregation of the tau-derived peptide VQIVYK (important for the aggregation of tau into paired helical filaments, which form the tangles found in AD and related disorders), and protected cognitive functions in a model of ADNP deficiency. With AD being the major tauopathy, novel NAP derivatives that reduce tauopathy and provide neuroprotection as well as cognitive protection are of scientific and clinical interest.

  4. Baby Naps: Daytime Sleep Tips

    MedlinePlus

    ... of sudden infant death syndrome: Diagnostic coding shifts, controversies regarding the sleeping environment, and new variables to ... infant-and-toddler-health/in-depth/baby-naps/art-20047421 . Mayo Clinic Footer Legal Conditions and Terms ...

  5. The napping behaviour of Australian university students.

    PubMed

    Lovato, Nicole; Lack, Leon; Wright, Helen

    2014-01-01

    The purpose of this study was to evaluate the self-reported sleep and napping behaviour of Australian university students and the relationship between napping and daytime functioning. A sample of 280 university first-year psychology students (median age  = 19.00 years) completed a 6-item napping behaviour questionnaire, a 12-item Daytime Feelings and Functioning Scale, the Pittsburg Sleep Quality Index and the Epworth Sleepiness Scale. Results indicated that 53.6% of students reported napping with 34% napping at least 1-2 times per week, and 17% napping three or more occasions per week. Long naps, those over 30 minutes, were taken by 77% of the napping students. Sixty-one percent of students reported they took long naps during the post-lunch dip period, from 2-4 pm. Students who nap at least once per week reported significantly more problems organizing their thoughts, gaining motivation, concentrating, and finishing tasks than students who did not nap. Students who napped also felt significantly more sleepy and depressed when compared to students who did not nap. The results also indicated that nap frequency increased with daytime sleepiness. The majority of students (51%) reported sleeping 6-7 hours per night or less. Overall, the results from this study suggest that among this population of Australian first-year university students habitual napping is common and may be used in an attempt to compensate for the detrimental effects of excessive sleepiness.

  6. NAPS-MS

    PubMed Central

    Gudesblatt, Mark; Kresa-Reahl, Kiren; Brandes, David W.; Sater, Pamela

    2016-01-01

    Background: Patients with multiple sclerosis (MS) have higher rates of fatigue, mood disturbance, and cognitive impairments than healthy populations. Disease-modifying agents may affect sleep. Although patients taking natalizumab often show improvement in fatigue during the first year of therapy, the mechanism behind this effect is unknown. The aim of the NAPS-MS study was to investigate whether natalizumab affected objective measures of sleep as determined by polysomnography (PSG) and multiple sleep latency testing (MSLT) in patients with MS with fatigue or sleepiness initiating therapy. Additional goals were to evaluate changes in measures of fatigue, mood, and cognition and to correlate these measures with objective sleep measures. Methods: Patients underwent PSG and MSLT before their first natalizumab infusion and after their seventh. Patients completed the Modified Fatigue Impact Scale, Fatigue Severity Scale (FSS), Epworth Sleepiness Scale (ESS), and visual analogue scale for fatigue (VAS-F) at their first, fourth, and seventh natalizumab infusions. NeuroTrax cognitive tests and the Hospital Anxiety and Depression Scale (HADS) were performed at the first and seventh natalizumab infusions. Results: Changes in sleep efficiency, wakefulness after sleep onset, and multiple sleep latency from baseline to 6 months of therapy did not reach significance. The FSS, VAS-F, ESS, and HADS scores were significantly improved after 6 months of therapy; cognitive scores were not significantly improved. Conclusions: Although treatment with natalizumab was associated with improvements in fatigue, sleepiness, and mood, changes in objective measures of sleep were not significant. PMID:27551242

  7. Benefits of napping in healthy adults: impact of nap length, time of day, age, and experience with napping.

    PubMed

    Milner, Catherine E; Cote, Kimberly A

    2009-06-01

    Napping is a cross-cultural phenomenon which occurs across the lifespan. People vary widely in the frequency with which they nap as well as the improvements in alertness and well-being experienced. The systematic study of daytime napping is important to understand the benefits in alertness and performance that may be accrued from napping. This review paper investigates factors that affect the benefits of napping such as duration and temporal placement of the nap. In addition, the influence of subject characteristics such as age and experience with napping is examined. The focus of the review is on benefits for healthy individuals with regular sleep/wake schedules rather than for people with sleep or medical disorders. The goal of the review is to summarize the type of performance improvements that result from napping, critique the existing studies, and make recommendations for future research.

  8. Mandatory Nap Times and Group Napping Patterns in Child Care: An Observational Study.

    PubMed

    Staton, Sally L; Smith, Simon S; Hurst, Cameron; Pattinson, Cassandra L; Thorpe, Karen J

    2017-01-01

    Policy provision for naps is typical in child care settings, but there is variability in the practices employed. One practice that might modify children's early sleep patterns is the allocation of a mandatory nap time in which all children are required to lie on their beds without alternate activity permitted. There is currently limited evidence of the effects of such practices on children's napping patterns. This study examined the association between duration of mandatory nap times and group-level napping patterns in child care settings. Observations were undertaken in a community sample of 113 preschool rooms with a scheduled nap time (N = 2,114 children). Results showed that 83.5% of child care settings implemented a mandatory nap time (range = 15-145 min) while 14.2% provided alternate activities for children throughout the nap time period. Overall, 31% of children napped during nap times. Compared to rooms with ≤ 30 min of mandatory nap time, rooms with 31-60 min and > 60 min of mandatory nap time had a two-and-a-half and fourfold increase, respectively, in the proportion of children napping. Nap onset latency did not significantly differ across groups. Among preschool children, exposure to longer mandatory nap times in child care may increase incidence of napping.

  9. Insomnia and daytime napping in older adults.

    PubMed

    Ancoli-Israel, Sonia; Martin, Jennifer L

    2006-07-15

    Insomnia, daytime sleepiness, and napping are all highly prevalent among the elderly, reflecting changes in sleep architecture, sleep efficiency, sleep quality, and circadian sleep-wake cycles. Insomnia is sometimes associated with subjective daytime sleepiness, as well as other clinical and socioeconomic consequences. The daytime sleepiness will at times lead to napping. Although napping is viewed as a common age-related occurrence, little is known about its benefits or consequences. Factors reported to be contributors to daytime napping include sleep-maintenance difficulty and sleep fragmentation with consequent daytime sleepiness, nighttime use of long-acting sedating agents, daytime use of sedating medications, and dementia. However, a correlation between sleep disturbance and daytime napping has not been consistently observed. Whether napping is beneficial, neutral, or detrimental is an important issue, in light of conflicting findings regarding the impact of daytime napping on nighttime sleep and recent reports of an association between napping and adverse clinical outcomes, including increased mortality risk. Further research is needed to determine whether there is a cause-and-effect relationship between napping and insomnia, and between napping and adverse clinical outcomes, and to explore the clinical implications of improving insomnia and reducing daytime napping. Clinical evaluations of hypnotic agents should assess efficacy for both improving insomnia symptoms (particularly sleep-maintenance difficulty, in the case of elderly patients) and reducing daytime sleepiness that would lead to inadvertent napping.

  10. Alertness management in flight operations - Strategic napping

    NASA Technical Reports Server (NTRS)

    Rosekind, Mark R.; Gander, Philippa H.; Dinges, David F.

    1991-01-01

    Strategic napping in two different flight operation environments is considered to illustrate its application as a fatigue countermeasure. Data obtained from commercial short-haul and long-haul operations demonstrated the utility and current practices of strategic napping. A preplanned cockpit nap acted as an acute 'safety valve' for the sleep loss, circadian disruption, and fatigue that occurs in long-haul flying.

  11. The periplasmic nitrate reductase in Shewanella: the resolution, distribution and functional implications of two NAP isoforms, NapEDABC and NapDAGHB.

    PubMed

    Simpson, Philippa J L; Richardson, David J; Codd, Rachel

    2010-02-01

    In the bacterial periplasm, the reduction of nitrate to nitrite is catalysed by a periplasmic nitrate reductase (NAP) system, which is a species-dependent assembly of protein subunits encoded by the nap operon. The reduction of nitrate catalysed by NAP takes place in the 90 kDa NapA subunit, which contains a Mo-bis-molybdopterin guanine dinucleotide cofactor and one [4Fe-4S] iron-sulfur cluster. A review of the nap operons in the genomes of 19 strains of Shewanella shows that most genomes contain two nap operons. This is an unusual feature of this genus. The two NAP isoforms each comprise three isoform-specific subunits - NapA, a di-haem cytochrome NapB, and a maturation chaperone NapD - but have different membrane-intrinsic subunits, and have been named NAP-alpha (NapEDABC) and NAP-beta (NapDAGHB). Sixteen Shewanella genomes encode both NAP-alpha and NAP-beta. The genome of the vigorous denitrifier Shewanella denitrificans OS217 encodes only NAP-alpha and the genome of the respiratory nitrate ammonifier Shewanella oneidensis MR-1 encodes only NAP-beta. This raises the possibility that NAP-alpha and NAP-beta are associated with physiologically distinct processes in the environmentally adaptable genus Shewanella.

  12. Peninsula Academies.

    ERIC Educational Resources Information Center

    Raby, Marilyn E.

    1984-01-01

    Describes the Peninsula Academies program developed by the Sequoia (California) Union High School District and 27 local high technology companies to help educationally disadvantaged students break the pattern of low academic achievement, lack of skills, and chronic unemployment. (FL)

  13. Napping: Do's and Don'ts for Healthy Adults

    MedlinePlus

    Healthy Lifestyle Adult health Napping isn't just for children. Understand the pros and cons of napping and the best way to take a nap. ... you might be thinking about taking a nap. Napping at the wrong time of day or for ...

  14. Role of individual nap gene cluster products in NapC-independent nitrate respiration of Wolinella succinogenes.

    PubMed

    Kern, Melanie; Mager, Anke M; Simon, Jörg

    2007-11-01

    Bacterial nap gene clusters, encoding periplasmic nitrate reductase (NapA), are complex and diverse, and the composition of the electron transport chain donating electrons to NapA is poorly characterized in most organisms. Exceptionally, Wolinella succinogenes transfers electrons from formate via the menaquinone pool to NapA independently of a membrane-bound c-type cytochrome of the NapC family. The role of individual ORFs of the W. succinogenes napAGHBFLD gene cluster is assessed here by characterizing in-frame gene inactivation mutants. The ability of the mutants to grow by nitrate respiration was tested and their NapA content and specific nitrate reductase activity were determined. The napB and napD gene products proved to be essential for nitrate respiration, with NapD being required for the production of mature NapA. Inactivation of either subunit of the putative membrane-bound menaquinol dehydrogenase complex NapGH almost abolished growth by nitrate respiration. Substitution of the twin-arginine sequence of NapG had the same effect as absence of NapG. Phenotypes of mutants lacking either NapF or NapL suggest that both proteins function in NapA assembly and/or export. The data substantiate the current model of the composition of the NapC-independent electron transport chain as well as of NapA maturation, and indicate the presence of an alternative electron transport pathway to NapA.

  15. Periplasmic nitrate reduction in Wolinella succinogenes: cytoplasmic NapF facilitates NapA maturation and requires the menaquinol dehydrogenase NapH for membrane attachment.

    PubMed

    Kern, Melanie; Simon, Jörg

    2009-08-01

    Various nitrate-reducing bacteria produce proteins of the periplasmic nitrate reductase (Nap) system to catalyse electron transport from the membraneous quinol pool to the periplasmic nitrate reductase NapA. The composition of the corresponding nap gene clusters varies but, in addition to napA, genes encoding at least one membrane-bound quinol dehydrogenase module (NapC and/or NapGH) are regularly present. Moreover, some nap loci predict accessory proteins such as the iron-sulfur protein NapF, whose function is poorly understood. Here, the role of NapF in nitrate respiration of the Epsilonproteobacterium Wolinella succinogenes was examined. Immunoblot analysis showed that NapF is located in the membrane fraction in nitrate-grown wild-type cells whereas it was found to be a soluble cytoplasmic protein in a napH deletion mutant. This finding indicates the formation of a membrane-bound NapGHF complex that is likely to catalyse NapH-dependent menaquinol oxidation and electron transport to the iron-sulfur adaptor proteins NapG and NapF, which are located on the periplasmic and cytoplasmic side of the membrane, respectively. The cysteine residues of a CX(3)CP motif and of the C-terminal tetra-cysteine cluster of NapH were found to be required for interaction with NapF. A napF deletion mutant accumulated the catalytically inactive cytoplasmic NapA precursor, suggesting that electron flow or direct interaction between NapF and NapA facilitated NapA assembly and/or export. On the other hand, NapA maturation and activity was not impaired in the absence of NapH, demonstrating that soluble NapF is functional. Each of the four tetra-cysteine motifs of NapF was modified but only one motif was found to be essential for efficient NapA maturation. It is concluded that the NapGHF complex plays a multifunctional role in menaquinol oxidation, electron transfer to periplasmic NapA and maturation of the cytoplasmic NapA precursor.

  16. Daytime nap controls toddlers’ nighttime sleep

    PubMed Central

    Nakagawa, Machiko; Ohta, Hidenobu; Nagaoki, Yuko; Shimabukuro, Rinshu; Asaka, Yoko; Takahashi, Noriko; Nakazawa, Takayo; Kaneshi, Yousuke; Morioka, Keita; Oishi, Yoshihisa; Azami, Yuriko; Ikeuchi, Mari; Takahashi, Mari; Hirata, Michio; Ozawa, Miwa; Cho, Kazutoshi; Kusakawa, Isao; Yoda, Hitoshi

    2016-01-01

    Previous studies have demonstrated that afternoon naps can have a negative effect on subsequent nighttime sleep in children. These studies have mainly been based on sleep questionnaires completed by parents. To investigate the effect of napping on such aspects of sleep quality, we performed a study in which child activity and sleep levels were recorded using actigraphy. The parents were asked to attach actigraphy units to their child’s waist by an adjustable elastic belt and complete a sleep diary for 7 consecutive days. 50 healthy young toddlers of approximately 1.5 years of age were recruited. There was a significant negative correlation between nap duration and both nighttime sleep duration and sleep onset time, suggesting that long nap sleep induces short nighttime sleep duration and late sleep onset time. We also found a significant negative correlation between nap timing and nighttime sleep duration and also a significant positive correlation between nap timing and sleep onset time, suggesting that naps in the late afternoon also lead to short nighttime sleep duration and late sleep onset. Our findings suggest that duration-controlled naps starting early in the afternoon can induce a longer nighttime sleep in full-term infants of approximately 1.5 years of age. PMID:27277329

  17. Daytime nap controls toddlers' nighttime sleep.

    PubMed

    Nakagawa, Machiko; Ohta, Hidenobu; Nagaoki, Yuko; Shimabukuro, Rinshu; Asaka, Yoko; Takahashi, Noriko; Nakazawa, Takayo; Kaneshi, Yousuke; Morioka, Keita; Oishi, Yoshihisa; Azami, Yuriko; Ikeuchi, Mari; Takahashi, Mari; Hirata, Michio; Ozawa, Miwa; Cho, Kazutoshi; Kusakawa, Isao; Yoda, Hitoshi

    2016-06-09

    Previous studies have demonstrated that afternoon naps can have a negative effect on subsequent nighttime sleep in children. These studies have mainly been based on sleep questionnaires completed by parents. To investigate the effect of napping on such aspects of sleep quality, we performed a study in which child activity and sleep levels were recorded using actigraphy. The parents were asked to attach actigraphy units to their child's waist by an adjustable elastic belt and complete a sleep diary for 7 consecutive days. 50 healthy young toddlers of approximately 1.5 years of age were recruited. There was a significant negative correlation between nap duration and both nighttime sleep duration and sleep onset time, suggesting that long nap sleep induces short nighttime sleep duration and late sleep onset time. We also found a significant negative correlation between nap timing and nighttime sleep duration and also a significant positive correlation between nap timing and sleep onset time, suggesting that naps in the late afternoon also lead to short nighttime sleep duration and late sleep onset. Our findings suggest that duration-controlled naps starting early in the afternoon can induce a longer nighttime sleep in full-term infants of approximately 1.5 years of age.

  18. NAP (davunetide) provides functional and structural neuroprotection.

    PubMed

    Gozes, Illana

    2011-01-01

    NAP (davunetide) is an eight amino acid peptide (NAPVSIPQ) that has been shown to provide potent neuroprotection, in vitro and in vivo. In human clinical trials, NAP has been shown to increase memory scores in patients suffering from amnestic mild cognitive impairment, a precursor to Alzheimer's disease and to enhance functional daily behaviors in schizophrenia patients. NAP is derived from activity-dependent neuroprotective protein (ADNP) a molecule that is essential for brain formation, interacting with chromatin associated protein alpha and the chromatin remodeling complex SWI/SNF and regulating >400 genes during embryonic development. Partial loss in ADNP results in cognitive deficits and pathology of the microtubule associated protein tau (tauopathy) that is ameliorated in part by NAP replacement therapy. Recent studies increased the scope of NAP neuroprotection and provided further insights into the NAP mechanisms of action. Thus, it has been hypothesized that the presence of tau on axonal microtubules renders them notably less sensitive to the microtubule-severing protein katanin, and NAP was shown to protect microtubules from katanin disruption in the face of reduced tau expression. Parallel studies showed that NAP reduced the number of apoptotic neurons through activation of PI-3K/Akt pathway in the cortical plate or both PI-3K/Akt and MAPK/MEK1 kinases in the white matter. The interaction of these disparate yet complementary pathways is the subject of future studies toward human brain neuroprotection in the clinical scenario.

  19. America's Service Academies, Your Service Academies

    ERIC Educational Resources Information Center

    Born, Dana H.; Phillips, Andrew T.; Trainor, Timothy E.

    2012-01-01

    The United States Air Force Academy, United States Naval Academy, and United States Military Academy are America's three largest service academies. They are "the" primary undergraduate institutions and commissioning sources that educate and develop the officers who are expected to lead this nation's armed forces. They are special places that have…

  20. Daydreams and nap dreams: Content comparisons.

    PubMed

    Carr, Michelle; Nielsen, Tore

    2015-11-01

    Differences between nighttime REM and NREM dreams are well-established but only rarely are daytime REM and NREM nap dreams compared with each other or with daydreams. Fifty-one participants took daytime naps (with REM or NREM awakenings) and provided both waking daydream and nap dream reports. They also provided ratings of their bizarreness, sensory experience, and emotion intensity. Recall rates for REM (96%) and NREM (89%) naps were elevated compared to typical recall rates for nighttime dreams (80% and 43% respectively), suggesting an enhanced circadian influence. All attribute ratings were higher for REM than for NREM dreams, replicating findings for nighttime dreams. Compared with daydreams, NREM dreams had lower ratings for emotional intensity and sensory experience while REM dreams had higher ratings for bizarreness and sensory experience. Results support using daytime naps in dream research and suggest that there occurs selective enhancement and inhibition of specific dream attributes by REM, NREM and waking state mechanisms.

  1. The effect of nap frequency on daytime sleep architecture.

    PubMed

    McDevitt, Elizabeth A; Alaynick, William A; Mednick, Sara C

    2012-08-20

    It is well documented that the quality and quantity of prior sleep influence future sleep. For instance, nocturnal sleep restriction leads to an increase in slow wave sleep (SWS) (i.e. SWS rebound) during a subsequent sleep period. However, few studies have examined how prior napping affects daytime sleep architecture. Because daytime naps are recommended for management of disrupted sleep, understanding the impact of napping on subsequent sleep may be important. We monitored sleep-wake patterns for one week with actigraphy followed by a 75-minute polysomnographically-recorded nap. We found that greater nap frequency was correlated with increased Stage 1 and decreased SWS. We categorized subjects based on nap frequency during the prior week (0 nap, 1 to 2 naps, and 3 to 4 naps) and found differences in Stage 1, Stage 2, and SWS between groups. Subjects who took no naps had the greatest amount of SWS, those who took 1 to 2 naps had the most Stage 2 sleep, and those who took 3 to 4 naps had the most Stage 1. While correlations were not found between nap frequency and nocturnal sleep measures, frequent napping was associated with increased subjective sleepiness. Therefore, frequent napping appears to be associated with lighter daytime sleep and increased sleepiness during the day. Speculatively, low levels of daytime sleepiness and increased SWS in non-nappers may help explain why these individuals choose not to nap.

  2. The Timing of the Circadian Clock and Sleep Differ between Napping and Non-Napping Toddlers.

    PubMed

    Akacem, Lameese D; Simpkin, Charles T; Carskadon, Mary A; Wright, Kenneth P; Jenni, Oskar G; Achermann, Peter; LeBourgeois, Monique K

    2015-01-01

    The timing of the internal circadian clock shows large inter-individual variability across the lifespan. Although the sleep-wakefulness pattern of most toddlers includes an afternoon nap, the association between napping and circadian phase in early childhood remains unexplored. This study examined differences in circadian phase and sleep between napping and non-napping toddlers. Data were collected on 20 toddlers (34.2±2.0 months; 12 females; 15 nappers). Children followed their habitual napping and non-napping sleep schedules (monitored with actigraphy) for 5 days before an in-home salivary dim light melatonin onset (DLMO) assessment. On average, napping children fell asleep during their nap opportunities on 3.6±1.2 of the 5 days before the DLMO assessment. For these napping children, melatonin onset time was 38 min later (p = 0.044; d = 0.93), actigraphically-estimated bedtime was 43 min later (p = 0.014; d = 1.24), sleep onset time was 59 min later (p = 0.006; d = 1.46), and sleep onset latency was 16 min longer (p = 0.030; d = 1.03) than those not napping. Midsleep and wake time did not differ by napping status. No difference was observed in the bedtime, sleep onset, or midsleep phase relationships with DLMO; however, the wake time phase difference was 47 min smaller for napping toddlers (p = 0.029; d = 1.23). On average, nappers had 69 min shorter nighttime sleep durations (p = 0.006; d = 1.47) and spent 49 min less time in bed (p = 0.019; d = 1.16) than non-nappers. Number of days napping was correlated with melatonin onset time (r = 0.49; p = 0.014). Our findings indicate that napping influences individual variability in melatonin onset time in early childhood. The delayed bedtimes of napping toddlers likely permits light exposure later in the evening, thereby delaying the timing of the clock and sleep. Whether the early developmental trajectory of circadian phase involves an advance associated with the decline in napping is a question necessitating

  3. Naps May Sharpen a Preschooler's Language Skills

    MedlinePlus

    ... fullstory_163510.html Naps May Sharpen a Preschooler's Language Skills Kids who slept after learning new verbs ... have an advantage when it comes to developing language skills, a new study suggests. Researchers assessed 39 ...

  4. Drill Presses.

    ERIC Educational Resources Information Center

    Engelbrecht, Nancy; And Others

    These instructional materials provide an orientation to the drill press for use at the postsecondary level. The first of seven sections lists seven types of drill presses. The second section identifies 14 drill press parts. The third section lists 21 rules for safe use of drilling machines. The fourth section identifies the six procedures for…

  5. Words to Sleep On: Naps Facilitate Verb Generalization in Habitually and Nonhabitually Napping Preschoolers.

    PubMed

    Sandoval, Michelle; Leclerc, Julia A; Gómez, Rebecca L

    2017-01-27

    A nap soon after encoding leads to better learning in infancy. However, whether napping plays the same role in preschoolers' learning is unclear. In Experiment 1 (N = 39), 3-year-old habitual and nonhabitual nappers learned novel verbs before a nap or a period of wakefulness and received a generalization test examining word extension to novel actors after 24 hr. Only habitual and nonhabitual nappers who napped after learning generalized 24 hr later. In Experiment 2 (N = 40), children learned the same verbs but were tested within 2-3 min of training. Here, habitual and nonhabitual nappers retained the mappings but did not generalize. The results suggest that naps consolidate weak learning that habitual and nonhabitual nappers would otherwise forget over periods of wakefulness.

  6. Daytime naps improve motor imagery learning.

    PubMed

    Debarnot, Ursula; Castellani, Eleonora; Valenza, Gaetano; Sebastiani, Laura; Guillot, Aymeric

    2011-12-01

    Sleep is known to contribute to motor memory consolidation. Recent studies have provided evidence that a night of sleep plays a similar functional role following motor imagery (MI), while the simple passage of time does not result in performance gains. Here, we examined the benefits of a daytime nap on motor memory consolidation after MI practice. Participants were trained by MI on an explicitly known sequence of finger movements at 11:00. Half of the participants were then subjected (at 14:00) to either a short nap (10 min of stage 2 sleep) or a long nap (60-90 min, including slow wave sleep and rapid eye movement sleep). We also collected data from both quiet and active rest control groups. All participants remained in the lab until being retested at 16:00. The data revealed that a daytime nap after imagery practice improved motor performance and, therefore, facilitated motor memory consolidation, as compared with spending a similar time interval in the wake state. Interestingly, the results revealed that both short and long naps resulted in similar delayed performance gains. The data might also suggest that the presence of slow wave and rapid eye movement sleep does not provide additional benefits for the sleep-dependent motor skill consolidation following MI practice.

  7. NAPS: Network Analysis of Protein Structures

    PubMed Central

    Chakrabarty, Broto; Parekh, Nita

    2016-01-01

    Traditionally, protein structures have been analysed by the secondary structure architecture and fold arrangement. An alternative approach that has shown promise is modelling proteins as a network of non-covalent interactions between amino acid residues. The network representation of proteins provide a systems approach to topological analysis of complex three-dimensional structures irrespective of secondary structure and fold type and provide insights into structure-function relationship. We have developed a web server for network based analysis of protein structures, NAPS, that facilitates quantitative and qualitative (visual) analysis of residue–residue interactions in: single chains, protein complex, modelled protein structures and trajectories (e.g. from molecular dynamics simulations). The user can specify atom type for network construction, distance range (in Å) and minimal amino acid separation along the sequence. NAPS provides users selection of node(s) and its neighbourhood based on centrality measures, physicochemical properties of amino acids or cluster of well-connected residues (k-cliques) for further analysis. Visual analysis of interacting domains and protein chains, and shortest path lengths between pair of residues are additional features that aid in functional analysis. NAPS support various analyses and visualization views for identifying functional residues, provide insight into mechanisms of protein folding, domain-domain and protein–protein interactions for understanding communication within and between proteins. URL:http://bioinf.iiit.ac.in/NAPS/. PMID:27151201

  8. Nap-Dependent Learning in Infants

    ERIC Educational Resources Information Center

    Hupbach, Almut; Gomez, Rebecca L.; Bootzin, Richard R.; Nadel, Lynn

    2009-01-01

    Sleep has been shown to aid a variety of learning and memory processes in adults (Stickgold, 2005 ). Recently, we showed that infants' learning also benefits from subsequent sleep such that infants who nap are able to abstract the general grammatical pattern of a briefly presented artificial language (Gomez, Bootzin & Nadel, 2006 ). In the present…

  9. American Academy of Dermatology

    MedlinePlus

    ... Us Media contacts Advertising contacts AAD logo Advertising, marketing and sponsorships Legal notice Copyright © 2017 American Academy ... prohibited without prior written permission. AAD logo Advertising, marketing and sponsorships Legal notice Copyright © 2017 American Academy ...

  10. Napping after complex motor learning enhances juggling performance.

    PubMed

    Morita, Yuko; Ogawa, Keiko; Uchida, Sunao

    2016-01-01

    The present study examined whether a nap after complex motor learning enhanced the following day's physical performance. Eighteen volunteers met the inclusion criteria and were assigned to either a nap (n=9; men=5; mean age=21.0±1.5) or no-nap group (n=9; men=5; mean age=21.9±0.3). Participants practiced juggling in the morning and were tested immediately afterwards. Participants of the nap group were given a 70-minute nap opportunity after juggling practice, while the no-nap group stayed awake. Juggling performance was then tested in the evening (retest-1) and the next morning (retest-2). Two-way analysis of variance (group: nap, no-nap×time: test, retest-1, retest-2) found there was a significant effect of test time and a significant group×time interaction. The juggling performance of both groups improved from test to retest-1, respectively. However, the juggling performance level of the nap group was higher than that of the no-nap group at the retest-1. As predicted, a nap promptly after learning motor skills was associated with subsequently improved performance. Moreover, the juggling performance of the nap group showed additional significant improvements in the retest-2. In the no-nap group, however, there were no significant improvements in the juggling performance after nocturnal sleep. These results demonstrate that the benefits of a nap following learning were further enhanced after nocturnal sleep. The present results may provide justification for introducing nap periods into daily athletic training as an active method to improve performance.

  11. Career Academy Course Sequences.

    ERIC Educational Resources Information Center

    Markham, Thom; Lenz, Robert

    This career academy course sequence guide is designed to give teachers a quick overview of the course sequences of well-known career academy and career pathway programs from across the country. The guide presents a variety of sample course sequences for the following academy themes: (1) arts and communication; (2) business and finance; (3)…

  12. Academies and School Diversity

    ERIC Educational Resources Information Center

    Curtis, Andrew

    2009-01-01

    This article considers the implications of Academies for the diversity of schooling in England. It seeks to establish the extent to which Academies are distinctive compared to other types of state secondary schools and whether this has been affected by a number of recent reforms. Different types of Academies are also be examined. Previous work in…

  13. Why a Steiner Academy?

    ERIC Educational Resources Information Center

    Avison, Kevin

    2008-01-01

    This article examines the curious position of the Academy model in the English school system and how a potential Hereford Steiner Waldorf Academy might figure in this. It sketches the background to the Steiner movement in the UK and goes on to set out the key aspirations and concerns of Steiner educators regarding an Academy. The article provides…

  14. Sleep spindles in midday naps enhance learning in preschool children.

    PubMed

    Kurdziel, Laura; Duclos, Kasey; Spencer, Rebecca M C

    2013-10-22

    Despite the fact that midday naps are characteristic of early childhood, very little is understood about the structure and function of these sleep bouts. Given that sleep benefits memory in young adults, it is possible that naps serve a similar function for young children. However, children transition from biphasic to monophasic sleep patterns in early childhood, eliminating the nap from their daily sleep schedule. As such, naps may contain mostly light sleep stages and serve little function for learning and memory during this transitional age. Lacking scientific understanding of the function of naps in early childhood, policy makers may eliminate preschool classroom nap opportunities due to increasing curriculum demands. Here we show evidence that classroom naps support learning in preschool children by enhancing memories acquired earlier in the day compared with equivalent intervals spent awake. This nap benefit is greatest for children who nap habitually, regardless of age. Performance losses when nap-deprived are not recovered during subsequent overnight sleep. Physiological recordings of naps support a role of sleep spindles in memory performance. These results suggest that distributed sleep is critical in early learning; when short-term memory stores are limited, memory consolidation must take place frequently.

  15. The Napping Company: bringing science to the workplace.

    PubMed

    Anthony, William A; Anthony, Camille W

    2005-01-01

    Increasing emphasis is currently being given to the importance of bringing knowledge gained from science into places where people live and work, in order to increase the impact science has on the general public's quality of life. Scientific findings about the positive impact of napping on mood and performance are an example of research generated knowledge that needs to be brought to the workplace. A major goal of the Napping Company (www.napping.com) is to bring the science of napping to the workers and the workplace so that employees and employers can act on this knowledge and change worker napping behavior and employer napping policies. The present paper overviews the challenges inherent in making scientific knowledge useful to how we live our lives. The Napping Company is guided by five principles of knowledge transfer in the company's attempts to disseminate and increase utilization of napping research. Examples are given to illustrate how the Napping Company has used these principles to bridge the gap between napping science and nap behavior and policies in the workplace.

  16. ICSU press

    NASA Astrophysics Data System (ADS)

    The International Council of Scientific Unions (ICSU) has established a publishing arm called ICSU Press. The Press is intended to complement the publishing activities of its member scientific unions in several ways: initiate special publications of research findings and new journals of reviews or research; advise, or act as publishers for, members requesting such service; and engage in copublishing ventures with international bodies outside of ICSU whose goals are consistent with ICSU's.Plans for ICSU Press also include preparation of television programs in cooperation with BBC-2 in Britain and PBS and ABC in the United States.

  17. CCP-NAP operator instructions at ANL

    SciTech Connect

    Tantillo, V.V.

    1986-03-01

    CCP-NAP Operator Instructions at ANL (ANL/TM 435) supplements the CCPGUIDE (Category 26) prepared by the National Magnetic Fusion Energy Computer Center (NMFECC), Lawrence Livermore National Laboratory. The instructions in CCP-NAP Operator Instructions at ANL serve as a reference guide for Computing Services operators when operator intervention is necessary for ANL's MFEnet Nodes located in Building 221, Room A-134. Use this document in conjunction with the CCPGUIDE. The CCPGUIDE, a guide for network operators, outlines the procedures for loading the Communications Control Processor (CCP) and monitoring its activity. The CCPGUIDE contains detailed information on Software Loading, System Status, System Monitoring, Command Summary, etc. The material in this document is the result of additional information provided by Jim Morton and Jim Gigliaridi (NMFECC) when our Argonne National Laboratory/Computing Services MFEnet Nodes were installed. This document provides two formats: QUICK instructions and procedures, and detailed general text.

  18. NapA and NapB are the Aspergillus nidulans Nap/SET family members and NapB is a nuclear protein specifically interacting with importin alpha.

    PubMed

    Araújo-Bazán, Lidia; Fernández-Martínez, Javier; Ríos, Vivian Maythe de Los; Etxebeste, Oier; Albar, Juan Pablo; Peñalva, Miguel Angel; Espeso, Eduardo Antonio

    2008-03-01

    In eukaryotic cells, importin alpha is the major carrier for transport protein cargoes into the nucleus. We characterize here kapA, the single Aspergillus nidulans gene encoding an importin alpha. Using an affinity approach, we identify six potential interactors of KapA(50), a deleted version of KapA lacking the autoinhibitory importin-beta-binding domain. One such interactor is NapB, the A. nidulans orthologue of Saccharomyces cerevisiae Vps75p, a histone chaperone member of the Nap/SET family of proteins that additionally plays a cytosolic role in vacuolar protein sorting. NapB, but not its close relative NapA (the A. nidulans orthologue of yeast Nap1p) interacts directly with KapA(50) in pull down assays, despite the fact that NapB does not contain a classical nuclear localization sequence. NapB is a nuclear protein which exits nuclei at the onset of mitosis when two simultaneous mechanisms might be acting, the partial disassembly of the nuclear pore complexes and as yet unidentified posttranslational modification of NapB. The mitotic cytosolic localization of NapB might facilitate its putative role in the sorting of protein cargoes to the vacuole. In addition, we show that NapB and the mitotic B-type cyclin NimE compete for in vitro binding to KapA.

  19. Radiophase development in hot-pressed alkoxide-derived titanate ceramics for nuclear waste stabilization

    SciTech Connect

    Dickson, F.J. ); Mitamura, H. ); White, T.J. )

    1989-06-01

    This paper reports phase development as a function of hot-pressing temperature studied in alkoxide-derived titanate-based ceramics doped with a 10 wt% loading of a sodium-rich (NAR) and a sodium-poor (NAP) simulated high-level waste. Pyrochlore was found to be the most abundant phase in both calcine powders. A pseudobrookite phase existed metastably at hot-pressing temperatures between 890{degrees} and 920{degrees} C. After hot-pressing at 1100{degrees} C, the final phase assemblage for the NAP material consisted of zirconolite, hollandite-type, perovskite, alloy, and reduced rutile (Magneli phases). In addition, NAR samples contained hibonite, freudenbergite, and loveringite. Phase development was driven to completion over a very narrow temperature range ({le}50{degrees} C), beginning at 870{degrees} and 850{degrees} C for NAP and NAR, respectively, although full densification was not achieved below 1100{degrees} C. Both waste forms exhibited comparable microstructure and aqueous durability.

  20. Temporal Relationships Between Napping and Nocturnal Sleep in Healthy Adolescents.

    PubMed

    Jakubowski, Karen P; Hall, Martica H; Lee, Laisze; Matthews, Karen A

    2017-01-01

    Many adolescents do not achieve the recommended 9 hr of sleep per night and report daytime napping, perhaps because it makes up for short nocturnal sleep. This article tests temporal relationships between daytime naps and nighttime sleep as measured by actigraphy and diary among 236 healthy high school students during one school week. Mixed model analyses adjusted for age, race, and gender demonstrated that shorter actigraphy-assessed nocturnal sleep duration predicted longer napping (measured by actigraphy and diary) the next day. Napping (by actigraphy and diary) predicted shorter nocturnal sleep duration and worse sleep efficiency that night measured by actigraphy. Diary-reported napping also predicted poorer self-reported sleep quality that night. Frequent napping may interfere with nocturnal sleep during adolescence.

  1. To Nap, Perchance to DREAM: A Factor Analysis of College Students' Self-Reported Reasons for Napping.

    PubMed

    Duggan, Katherine A; McDevitt, Elizabeth A; Whitehurst, Lauren N; Mednick, Sara C

    2016-06-27

    Although napping has received attention because of its associations with health and use as a method to understand the function of sleep, to our knowledge no study has systematically and statistically assessed reasons for napping. Using factor analysis, we determined the underlying structure of reasons for napping in diverse undergraduates (N = 430, 59% female) and examined their relationships with self-reported sleep, psychological health, and physical health. The five reasons for napping can be summarized using the acronym DREAM (Dysregulative, Restorative, Emotional, Appetitive, and Mindful). Only Emotional reasons for napping were uniformly related to lower well-being. The use of factor analysis raises possibilities for future research, including examining the stability, structure, and psychological and physical health processes related to napping throughout the lifespan.

  2. Talent Management in Academies

    ERIC Educational Resources Information Center

    Davies, Brent; Davies, Barbara J.

    2010-01-01

    Purpose: Academies are semi-autonomous schools set up outside the normal local government structures with sponsors from business and charity groups to create new and innovative ways of creating and sustaining school transformation. The aim of this paper is to assist in a strategic conversation within the academy movement on talent development.…

  3. Hydraulic Presses,

    DTIC Science & Technology

    1984-03-09

    flow of metal ( piercing in the ring, etc.) - 20-30 kgf/mm2. The efficiency of press, which considers losses to fluid friction in the conduit/manifold...the strong wear of the friction surfaces, have the low coefficient of friction , be - inert with respect to the material of mating members and working...stump or knock, impregnated with grease. Such sealings/packings/compactions have the <. high coefficient of friction , require rapid the suspenders and

  4. The experience of a Power Nap Center in the largest city of Brazil.

    PubMed

    Santos-Silva, Rogerio; Jankavski, Camila; Lorenzi-Filho, Geraldo

    2016-01-01

    We evaluated the frequency of naps and features of nappers who took a nap in a Power Nap Center located in downtown area of São Paulo. Company database was retrospectively analyzed and 4.625 naps were evaluated (January-December 2014). Most naps (57%) lasted 30 min. 33% of subjects took a nap more than once a week (73% male). Progressive growth in the number of naps across the months was observed (January=110 to December=505). Results suggest that the society is sleep deprived and taking a nap during the day could be an important strategy to improve quality of life and increase productivity.

  5. Extremely low polymerizability of a highly-divergent Chlamydomonas actin (NAP).

    PubMed

    Kato-Minoura, Takako

    2011-09-09

    Novel actin-like protein (NAP) is a highly divergent actin expressed in Chlamydomonas. With its low sequence similarity, it is uncertain whether NAP can polymerize into filaments. Here I assessed it by ectopically expressing enhanced green fluorescent protein-tagged NAP (EGFP-NAP) in cultured cells. EGFP-NAP was excluded from stress fibres but partially co-localized with endogenous actin in the cell periphery. In fluorescence recovery after photobleaching experiment, turnover rate of EGFP-NAP was similar to the estimated diffusion rate of monomeric actin. Therefore, EGFP-NAP likely accumulates by diffusion. These findings suggest that NAP has extremely poor ability to polymerize.

  6. Napping in College Students and Its Relationship with Nighttime Sleep

    ERIC Educational Resources Information Center

    Ye, Lichuan; Hutton Johnson, Stacy; Keane, Kathleen; Manasia, Michael; Gregas, Matt

    2015-01-01

    Objective: To examine the habit of napping and its relationship with nighttime sleep in college students. Participants: Four hundred and forty undergraduate students who responded to an anonymous online survey in April 2010. Methods: Three questions were asked to determine the frequency, length, and timing of napping during the past month. Sleep…

  7. Napping facilitates word learning in early lexical development.

    PubMed

    Horváth, Klára; Myers, Kyle; Foster, Russell; Plunkett, Kim

    2015-10-01

    Little is known about the role that night-time sleep and daytime naps play in early cognitive development. Our aim was to investigate how napping affects word learning in 16-month-olds. Thirty-four typically developing infants were assigned randomly to nap and wake groups. After teaching two novel object-word pairs to infants, we tested their initial performance with an intermodal preferential looking task in which infants are expected to increase their target looking time compared to a distracter after hearing its auditory label. A second test session followed after approximately a 2-h delay. The delay contained sleep for the nap group or no sleep for the wake group. Looking behaviour was measured with an automatic eye-tracker. Vocabulary size was assessed using the Oxford Communicative Development Inventory. A significant interaction between group and session was found in preferential looking towards the target picture. The performance of the nap group increased after the nap, whereas that of the wake group did not change. The gain in performance correlated positively with the expressive vocabulary size in the nap group. These results indicate that daytime napping helps consolidate word learning in infancy.

  8. Nap sleep spindle correlates of intelligence.

    PubMed

    Ujma, Péter P; Bódizs, Róbert; Gombos, Ferenc; Stintzing, Johannes; Konrad, Boris N; Genzel, Lisa; Steiger, Axel; Dresler, Martin

    2015-11-26

    Sleep spindles are thalamocortical oscillations in non-rapid eye movement (NREM) sleep, that play an important role in sleep-related neuroplasticity and offline information processing. Several studies with full-night sleep recordings have reported a positive association between sleep spindles and fluid intelligence scores, however more recently it has been shown that only few sleep spindle measures correlate with intelligence in females, and none in males. Sleep spindle regulation underlies a circadian rhythm, however the association between spindles and intelligence has not been investigated in daytime nap sleep so far. In a sample of 86 healthy male human subjects, we investigated the correlation between fluid intelligence and sleep spindle parameters in an afternoon nap of 100 minutes. Mean sleep spindle length, amplitude and density were computed for each subject and for each derivation for both slow and fast spindles. A positive association was found between intelligence and slow spindle duration, but not any other sleep spindle parameter. As a positive correlation between intelligence and slow sleep spindle duration in full-night polysomnography has only been reported in females but not males, our results suggest that the association between intelligence and sleep spindles is more complex than previously assumed.

  9. Physiological and evolutionary studies of NAP systems in Shewanella piezotolerans WP3.

    PubMed

    Chen, Ying; Wang, Fengping; Xu, Jun; Mehmood, Muhammad Aamer; Xiao, Xiang

    2011-05-01

    Most of the Shewanella species contain two periplasmic nitrate reductases (NAP-α and NAP-β), which is a unique feature of this genus. In the present study, the physiological function and evolutionary relationship of the two NAP systems were studied in the deep-sea bacterium Shewanella piezotolerans WP3. Both of the WP3 nap gene clusters: nap-α (napD1A1B1C) and nap-β (napD2A2B2) were shown to be involved in nitrate respiration. Phylogenetic analyses suggest that NAP-β originated earlier than NAP-α. Tetraheme cytochromes NapC and CymA were found to be the major electron deliver proteins, and CymA also served as a sole electron transporter towards nitrite reductase. Interestingly, a ΔnapA2 mutant with the single functional NAP-α system showed better growth than the wild-type strain, when grown in nitrate medium, and it had a selective advantage to the wild-type strain. On the basis of these results, we proposed the evolution direction of nitrate respiration system in Shewanella: from a single NAP-β to NAP-β and NAP-α both, followed by the evolution to a single NAP-α. Moreover, the data presented here will be very useful for the designed engineering of Shewanella for more efficient respiring capabilities for environmental bioremediation.

  10. Physiological and evolutionary studies of NAP systems in Shewanella piezotolerans WP3

    PubMed Central

    Chen, Ying; Wang, Fengping; Xu, Jun; Mehmood, Muhammad Aamer; Xiao, Xiang

    2011-01-01

    Most of the Shewanella species contain two periplasmic nitrate reductases (NAP-α and NAP-β), which is a unique feature of this genus. In the present study, the physiological function and evolutionary relationship of the two NAP systems were studied in the deep-sea bacterium Shewanella piezotolerans WP3. Both of the WP3 nap gene clusters: nap-α (napD1A1B1C) and nap-β (napD2A2B2) were shown to be involved in nitrate respiration. Phylogenetic analyses suggest that NAP-β originated earlier than NAP-α. Tetraheme cytochromes NapC and CymA were found to be the major electron deliver proteins, and CymA also served as a sole electron transporter towards nitrite reductase. Interestingly, a ΔnapA2 mutant with the single functional NAP-α system showed better growth than the wild-type strain, when grown in nitrate medium, and it had a selective advantage to the wild-type strain. On the basis of these results, we proposed the evolution direction of nitrate respiration system in Shewanella: from a single NAP-β to NAP-β and NAP-α both, followed by the evolution to a single NAP-α. Moreover, the data presented here will be very useful for the designed engineering of Shewanella for more efficient respiring capabilities for environmental bioremediation. PMID:21124486

  11. American Academy of Optometry

    MedlinePlus

    ... care through lifelong learning. Login Meetings Academy 2017 Chicago 2017 Call for Courses 2017 Exhibitor Information Scientific ... Our Partners About Community Home Join us in Chicago! October 11 - 14, 2017 at McCormick Place. More... ...

  12. American Academy of Audiology

    MedlinePlus

    ... CEU Manager by April 21. Audiology News 27 Mar Suppressors: Hearing Protection or Safety Threat? 22 Mar Is There a Genetic Component to Tinnitus? 03 Mar Celebrate World Hearing Day with the Academy! 23 ...

  13. American Academy of Ophthalmology

    MedlinePlus

    ... Plastic Surgery Center Laser Surgery Education Center Redmond Ethics Center Global Ophthalmology Guide Academy Publications EyeNet Ophthalmology Information for: International Ophthalmologists Media Medical Students Patients and Public Technicians and Nurses ...

  14. Napping reduces emotional attention bias during early childhood.

    PubMed

    Cremone, Amanda; Kurdziel, Laura B F; Fraticelli-Torres, Ada; McDermott, Jennifer M; Spencer, Rebecca M C

    2016-06-10

    Sleep loss alters processing of emotional stimuli in preschool-aged children. However, the mechanism by which sleep modifies emotional processing in early childhood is unknown. We tested the hypothesis that a nap, compared to an equivalent time spent awake, reduces biases in attention allocation to affective information. Children (n = 43; M = 55.40 months, SD = 8.05 months) completed a Dot Probe task, which provides a measure of attention biases to emotional stimuli, following a mid-day nap and an equivalent interval spent awake. No emotional attention biases emerged when children napped. However, when nap-deprived, children exhibited biases towards negative and positive stimuli. This emotional bias after wake was greater in children who napped habitually. Gender differences also emerged such that females were more attentive to positive emotional stimuli whereas males showed heightened attention to negative emotional stimuli, regardless of having napped or not. Moreover, greater slow wave activity (SWA) during the nap was associated with faster responding, which suggests that SWA may promote efficiency of attention allocation. A video abstract of this article can be viewed at: https://www.youtube.com/watch?v=JIoZ8mzxQgg.

  15. Tight binding of NAP-22 with acidic membrane lipids.

    PubMed

    Maekawa, Shohei; Kobayashi, Yuumi; Morita, Mitsuhiro; Suzaki, Toshinobu

    2015-07-23

    Recovery of various signal transduction molecules in the detergent-resistant membrane microdomain (DRM) fraction suggests the importance of this region in cellular functions. Insolubility of the outer leaflet of DRM to the non-ionic detergent is ascribed to the tight association of cholesterol and sphingolipid. Since, poor localization of sphingolipid is observed in the inner leaflet, the physicochemical background of the insolubility of the inner leaflet is hence still an enigma. NAP-22 (also called BASP1 or CAP-23) is a neuron-enriched calmodulin-binding protein and one of the major proteins in the DRM of the neuronal cell membrane. A previous study showed the presence of several lipids in a NAP-22 fraction after the process of extraction and column chromatography. In this study, the effect of lipid extraction on NAP-22 was studied through native-gel electrophoresis, ultracentrifugation, and electron microscopic observation. The mobility of NAP-22 in native-PAGE was shifted from low to high after delipidation. Delipidated NAP-22 bound phosphatidylserine (PS), phosphatidylinosotol, and ganglioside. Some part of the mixture of PS and NAP-22 was recovered in the insoluble fraction after Triton X-100 treatment and the addition of cholesterol enhanced the amount of NAP-22 in the insoluble fraction.

  16. Microtubules (tau) as an emerging therapeutic target: NAP (davunetide).

    PubMed

    Gozes, Illana

    2011-01-01

    This review focuses on the discovery of activity-dependent neuroprotective protein (ADNP) and the ensuing discovery of NAP (davunetide) toward clinical development with emphasis on microtubule protection. ADNP immunoreactivity was shown to occasionally decorate microtubules and ADNP silencing inhibited neurite outgrowth as measured by microtubule associated protein 2 (MAP2) labeling. ADNP knockout is lethal, while 50% reduction in ADNP (ADNP haploinsufficiency) resulted in the microtubule associated protein tau pathology coupled to cognitive dysfunction and neurodegeneration. NAP (davunetide), an eight amino acid peptide derived from ADNP partly ameliorated deficits associated with ADNP deficiency. NAP (davunetide) interacted with microtubules, protected against microtubule toxicity associated with zinc, nocodazole and oxidative stress in vitro and against tau pathology and MAP6 (stable tubuleonly polypeptide - STOP) pathology in vivo. NAP (davunetide) provided neurotrophic functions promoting neurite outgrowth as measured by increases in MAP2 immunoreactivity and synapse formation by increasing synaptophysin expression. NAP (davunetide) protection against neurodegeneration has recently been shown to extend to katanin-related microtubule disruption under conditions of tau deficiencies. In conclusion, NAP (davunetide) provided potent neuroprotection in a broad range of neurodegenerative models, protecting the neuroglial cytoskeleton in vitro and inhibiting tau pathology (tauopathy) in vivo. Based on these extensive preclinical results, davunetide (NAP) is now being evaluated in a Phase II/III study of the tauopathy, progressive supranuclear palsy (PSP); (Allon Therapeutics Inc.).

  17. A 30-Minute, but Not a 10-Minute Nighttime Nap is Associated with Sleep Inertia

    PubMed Central

    Hilditch, Cassie J.; Centofanti, Stephanie A.; Dorrian, Jillian; Banks, Siobhan

    2016-01-01

    Study Objectives: To assess sleep inertia following 10-min and 30-min naps during a simulated night shift. Methods: Thirty-one healthy adults (aged 21–35 y; 18 females) participated in a 3-day laboratory study that included one baseline (BL) sleep (22:00–07:00) and one experimental night involving randomization to either: total sleep deprivation (NO-NAP), a 10-min nap (10-NAP) or a 30-min nap (30-NAP). Nap opportunities ended at 04:00. A 3-min psychomotor vigilance task (PVT-B), digit-symbol substitution task (DSST), fatigue scale, sleepiness scale, and self-rated performance scale were undertaken pre-nap (03:00) and at 2, 17, 32, and 47 min post-nap. Results: The 30-NAP (14.7 ± 5.7 min) had more slow wave sleep than the 10-NAP (0.8 ± 1.5 min; P < 0.001) condition. In the NO-NAP condition, PVT-B performance was worse than pre-nap (4.6 ± 0.3 1/sec) at 47 min post-nap (4.1 ± 0.4 1/sec; P < 0.001). There was no change across time in the 10-NAP condition. In the 30-NAP condition, performance immediately deteriorated from pre-nap (4.3 ± 0.3 1/sec) and was still worse at 47 min post-nap (4.0 ± 0.5 1/sec; P < 0.015). DSST performance deteriorated in the NO-NAP (worse than pre-nap from 17 to 47 min; P < 0.008), did not change in the 10-NAP, and was impaired 2 min post-nap in the 30-NAP condition (P = 0.028). All conditions self-rated performance as better than pre-nap for all post-nap test points (P < 0.001). Conclusions: This study is the first to show that a 10-min (but not a 30-min) nighttime nap had minimal sleep inertia and helped to mitigate short-term performance impairment during a simulated night shift. Self-rated performance did not reflect objective performance following a nap. Citation: Hilditch CJ, Centofanti SA, Dorrian J, Banks S. A 30-minute, but not a 10-minute nighttime nap is associated with sleep inertia. SLEEP 2016;39(3):675–685. PMID:26715234

  18. The NapF protein of the Escherichia coli periplasmic nitrate reductase system: demonstration of a cytoplasmic location and interaction with the catalytic subunit, NapA.

    PubMed

    Nilavongse, Arjaree; Brondijk, T Harma C; Overton, Tim W; Richardson, David J; Leach, Emily R; Cole, Jeffrey A

    2006-11-01

    The periplasmic nitrate reductase of Escherichia coli is important during anaerobic growth in low-nitrate environments. The nap operon encoding this nitrate reductase comprises seven genes including a gene, napF, that encodes a putative cytoplasmic iron-sulphur protein of uncertain subcellular location and function. In this study, N-terminal sequence analysis, cell fractionation coupled with immunoblotting and construction of LacZ and PhoA fusion proteins were used together to establish that NapF is located in the E. coli cytoplasm. A bacterial two-hybrid protein-protein interaction system was used to demonstrate that NapF interacted in the cytoplasm with the terminal oxidoreductase NapA, but that it did not self-associate or interact with other electron-transport components of the Nap system, NapC, NapG or NapH, or with another cytoplasmic component, NapD. NapF, purified as a His(6)-tagged protein, exhibited spectral properties characteristic of an iron-sulphur protein. This protein was able to pull down NapA from soluble extracts of E. coli. A growth-based assay for NapF function in intact cell cultures was developed and applied to assess the effect of mutation of a number of conserved amino acids. It emerged that neither a highly conserved N-terminal double-arginine motif, nor a conserved proline motif, is essential for NapF-dependent growth. The combined data indicate that NapF plays one or more currently unidentified roles in the post-translational modification of NapA prior to the export of folded NapA via the twin-arginine translocation pathway into the periplasm.

  19. Electron transport to periplasmic nitrate reductase (NapA) of Wolinella succinogenes is independent of a NapC protein.

    PubMed

    Simon, Jörg; Sänger, Monica; Schuster, Stephan C; Gross, Roland

    2003-07-01

    The rumen bacterium Wolinella succinogenes grows by respiratory nitrate ammonification with formate as electron donor. Whereas the enzymology and coupling mechanism of nitrite respiration is well known, nitrate reduction to nitrite has not yet been examined. We report here that intact cells and cell fractions catalyse nitrate and chlorate reduction by reduced viologen dyes with high specific activities. A gene cluster encoding components of a putative periplasmic nitrate reductase system (napA, G, H, B, F, L, D) was sequenced. The napA gene was inactivated by inserting a kanamycin resistance gene cassette. The resulting mutant did not grow by nitrate respiration and did not reduce nitrate during growth by fumarate respiration, in contrast to the wild type. An antigen was detected in wild-type cells using an antiserum raised against the periplasmic nitrate reductase (NapA) from Paracoccus pantotrophus. This antigen was absent in the W. succinogenes napA mutant. It is concluded that the periplasmic nitrate reductase NapA is the only respiratory nitrate reductase in W. succinogenes, although a second nitrate-reducing enzyme is apparently induced in the napA mutant. The nap cluster of W. succinogenes lacks a napC gene whose product is thought to function in quinol oxidation and electron transfer to NapA in other bacteria. The W. succinogenes genome encodes two members of the NapC/NirT family, NrfH and FccC. Characterization of corresponding deletion mutants indicates that neither of these two proteins is required for nitrate respiration. A mutant lacking the genes encoding respiratory nitrite reductase (nrfHA) had wild-type properties with respect to nitrate respiration. A model of the electron transport chain of nitrate respiration is proposed in which one or more of the napF, G, H and L gene products mediate electron transport from menaquinol to the periplasmic NapAB complex. Inspection of the W. succinogenes genome sequence suggests that ammonia formation from

  20. Reduction of Nitrate in Shewanella oneidensis depends on atypical NAP and NRF systems with NapB as a preferred electron transport protein from CymA to NapA

    SciTech Connect

    Gao, Haichun; Yang, Zamin; Barua, Soumitra; Reed, Samantha B.; Romine, Margaret F.; Nealson, Kenneth H.; Fredrickson, Jim K.; Tiedje, James M.; Zhou, Jizhong

    2009-04-23

    In the genome of Shewanella oneidensis, a napDAGHB gene cluster encoding periplasmic nitrate reductase (NapA) and accessory proteins and an nrfA gene encoding periplasmic nitrite reductase (NrfA) have been identified. These two systems seem to be atypical because the genome lacks genes encoding cytoplasmic membrane electron transport proteins, NapC for NAP and NrfBCD/NrfH for NRF, respectively. Here, we present evidence that reduction of nitrate to ammonium in S. oneidensis is carried out by these atypical systems in a two-step manner. Transcriptional and mutational analyses suggest that CymA, a cytoplasmic membrane electron transport protein, is likely to be the functional replacement of both NapC and NrfH in S. oneidensis. Surprisingly, a strain devoid of napB encoding the small subunit of nitrate reductase exhibited the maximum cell density sooner than the wild type. Further characterization of this strain showed that nitrite was not detected as a free intermediate in its culture and NapB provides a fitness gain for S. oneidensis to compete for nitrate in the environments. On the basis results from mutational analyses of napA, napB, nrfA and napBnrfA in-frame deletion mutants, we propose that NapB is able to favor nitrate reduction by routing electrons to NapA exclusively.

  1. Reduction of nitrate in Shewanella oneidensis depends on atypical NAP and NRF systems with NapB as a preferred electron transport protein from CymA to NapA.

    PubMed

    Gao, Haichun; Yang, Zamin K; Barua, Soumitra; Reed, Samantha B; Romine, Margaret F; Nealson, Kenneth H; Fredrickson, James K; Tiedje, James M; Zhou, Jizhong

    2009-08-01

    In the genome of Shewanella oneidensis, a napDAGHB gene cluster encoding periplasmic nitrate reductase (NapA) and accessory proteins and an nrfA gene encoding periplasmic nitrite reductase (NrfA) have been identified. These two systems seem to be atypical because the genome lacks genes encoding cytoplasmic membrane electron transport proteins, NapC for NAP and NrfBCD/NrfH for NRF, respectively. Here, we present evidence that reduction of nitrate to ammonium in S. oneidensis is carried out by these atypical systems in a two-step manner. Transcriptional and mutational analyses suggest that CymA, a cytoplasmic membrane electron transport protein, is likely to be the functional replacement of both NapC and NrfH in S. oneidensis. Surprisingly, a strain devoid of napB encoding the small subunit of nitrate reductase exhibited the maximum cell density sooner than the wild type. Further characterization of this strain showed that nitrite was not detected as a free intermediate in its culture and NapB provides a fitness gain for S. oneidensis to compete for nitrate in the environments. On the basis results from mutational analyses of napA, napB, nrfA and napBnrfA in-frame deletion mutants, we propose that NapB is able to favor nitrate reduction by routing electrons to NapA exclusively.

  2. Press Start

    NASA Astrophysics Data System (ADS)

    Harteveld, Casper

    This level sets the stage for the design philosophy called “Triadic Game Design” (TGD). This design philosophy can be summarized with the following sentence: it takes two to tango, but it takes three to design a meaningful game or a game with a purpose. Before the philosophy is further explained, this level will first delve into what is meant by a meaningful game or a game with a purpose. Many terms and definitions have seen the light and in this book I will specifically orient at digital games that aim to have an effect beyond the context of the game itself. Subsequently, a historical overview is given of the usage of games with a serious purpose which starts from the moment we human beings started to walk on our feet till our contemporary society. It turns out that we have been using games for all kinds of non-entertainment purposes for already quite a long time. With this introductory material in the back of our minds, I will explain the concept of TGD by means of a puzzle. After that, the protagonist of this book, the game Levee Patroller, is introduced. Based on the development of this game, the idea of TGD, which stresses to balance three different worlds, the worlds of Reality, Meaning, and Play, came into being. Interested? Then I suggest to quickly “press start!”

  3. Photocopy of original nap (from feeder book), New York State ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photocopy of original nap (from feeder book), New York State Archives and Manuscripts, Albany, New York), Holnes Hutchinson, surveyor, 1834 - Glens Falls Feeder, Sluice, Along south side of Glens Falls Feeder between locks 10 & 20, Hudson Falls, Washington County, NY

  4. 7. VIEW SHOWING EAST ELEVATION OF OVERHEAD BRIDGE CONNECTING NAPPING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. VIEW SHOWING EAST ELEVATION OF OVERHEAD BRIDGE CONNECTING NAPPING BUILDING (LEFT) WITH CLOTH ROOM (RIGHT), LOOKING WEST - Massachusetts Mills, Cloth Room-Section 15, 95 Bridge Street, Lowell, Middlesex County, MA

  5. Practice and nap schedules modulate children's motor learning.

    PubMed

    Ren, Jie; Guo, Wei; Yan, Jin H; Liu, Guanmin; Jia, Fujun

    2016-01-01

    Night- or day-time sleep enhances motor skill acquisition. However, prominent issues remained about the circadian (time-of-day) and homeostatic (time since last sleep) effects of sleep on developmental motor learning. Therefore, we examined the effects of nap schedules and nap-test-intervals (NTIs) on the learning of finger tapping sequences on computer keyboards. Children aged 6-7, 8-9, and 10-11 years explicitly acquired the short and long tapping orders that share the same movement strings (4-2-3-1-4, 4-2-3-1-4-2-3-1-4). Following a constant 8- or 10-hr post-learning period in one of the four NTIs (2, 4, 5, 7 hr), children in the morning napping groups, the afternoon napping groups, or the waking group performed the original long sequence in retention test (4-2-3-1-4-2-3-1-4) and the mirrored-order sequence in transfer test (1-3-2-4-1-3-2-4-1). Age and treatment differences in the movement time (MT, ms) and sequence accuracy (SA, %) were compared during skill learning and in retrieval tests. Results suggest that practice or nap affects MT and SA in a greater extent for the younger learners than for the older learners. The circadian effects might not change nap-based skill learning. Importantly, the longer NTIs resulted in superior retention performance than the shorter ones, suggesting that children require a relatively longer post-nap period to form motor memory. Finally, nap-based motor learning was more marked in skill retention than in skill transfer. Brain development may play an important role in motor learning. Our discussion centers on memory consolidation and its relevance for skill acquisition from early to late childhood.

  6. Military Science and Academies,

    DTIC Science & Technology

    1981-10-06

    AD-AL06 093 FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFG OH F/G 5/9 MILITARY SCIENCE AND ACAO(MIES, CU) OCT 81 P A ROTMISTROY UNC LASIED FTO-ID(RS)T...1102-61IL FTD-ID(RS )T-1142-8l FOREIGN TECHNOLOGY DIVISION MILITARY SCIENCE AND ACADEMIES by P. A. Rotmistrov DTIC ELECTE NV2 1981 B Approved for...Distribution/ Availability Codes jAvail and/or Dist Special MILITARY SCIENCE AND ACADEMIES Chief Marshal of Armored Troops P. A. Rotmistrov The Central

  7. Napping: A public health issue. From epidemiological to laboratory studies.

    PubMed

    Faraut, Brice; Andrillon, Thomas; Vecchierini, Marie-Françoise; Leger, Damien

    2016-09-13

    Sleep specialists have proposed measures to counteract the negative short- and long-term consequences of sleep debt, and some have suggested the nap as a potential and powerful "public health tool". Here, we address this countermeasure aspect of napping viewed as an action against sleep deprivation rather than an action associated with poor health. We review the physiological functions that have been associated positively with napping in both public health and clinical settings (sleep-related accidents, work and school, and cardiovascular risk) and in laboratory-based studies with potential public health issues (cognitive performance, stress, immune function and pain sensitivity). We also discuss the circumstances in which napping-depending on several factors, including nap duration, frequency, and age-could be a potential public health tool and a countermeasure for sleep loss in terms of reducing accidents and cardiovascular events and improving sleep-restriction-sensitive working performance. However, the impact of napping and the nature of the sleep stage(s) involved still need to be evaluated, especially from the perspective of coping strategies in populations with chronic sleep debt, such as night and shift workers.

  8. Napping and the selective consolidation of negative aspects of scenes.

    PubMed

    Payne, Jessica D; Kensinger, Elizabeth A; Wamsley, Erin J; Spreng, R Nathan; Alger, Sara E; Gibler, Kyle; Schacter, Daniel L; Stickgold, Robert

    2015-04-01

    After information is encoded into memory, it undergoes an offline period of consolidation that occurs optimally during sleep. The consolidation process not only solidifies memories, but also selectively preserves aspects of experience that are emotionally salient and relevant for future use. Here, we provide evidence that an afternoon nap is sufficient to trigger preferential memory for emotional information contained in complex scenes. Selective memory for negative emotional information was enhanced after a nap compared with wakefulness in 2 control conditions designed to carefully address interference and time-of-day confounds. Although prior evidence has connected negative emotional memory formation to REM sleep physiology, we found that non-REM delta activity and the amount of slow wave sleep (SWS) in the nap were robustly related to the selective consolidation of negative information. These findings suggest that the mechanisms underlying memory consolidation benefits associated with napping and nighttime sleep are not always the same. Finally, we provide preliminary evidence that the magnitude of the emotional memory benefit conferred by sleep is equivalent following a nap and a full night of sleep, suggesting that selective emotional remembering can be economically achieved by taking a nap.

  9. NAP reduces murine microvascular endothelial cells proliferation induced by hyperglycemia.

    PubMed

    D'Amico, Agata Grazia; Scuderi, Soraya; Maugeri, Grazia; Cavallaro, Sebastiano; Drago, Filippo; D'Agata, Velia

    2014-11-01

    Hyperglycemia has been identified as a risk factor responsible for micro- and macrovascular complications in diabetes. NAP (Davunetide) is a peptide whose neuroprotective actions are widely demonstrated, although its biological role on endothelial dysfunctions induced by hyperglycemia remains uninvestigated. In the present study we hypothesized that NAP could play a protective role on hyperglycemia-induced endothelial cell proliferation. To this end we investigated the effects of NAP on an in vitro model of murine microvascular endothelial cells grown in high glucose for 7 days. The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and cyclin D1 protein expression analysis revealed that NAP treatment significantly reduces viability and proliferation of the cells. Hyperglycemia induced the activation of mitogen-activated protein kinase/extracellular signal-regulated protein kinase and/or phosphatidylinositol-3 kinase/Akt pathways in a time-dependent manner. NAP treatment reduced the phosphorylation levels of ERK and AKT in cells grown in high glucose. These evidences suggest that NAP might be effective in the regulation of endothelial dysfunction induced by hyperglycemia.

  10. Benjamin Franklin Street Academy.

    ERIC Educational Resources Information Center

    Wohl, Seth F.

    In this evaluation report of the sixth year of operation of the Benjamin Franklin-Urban League Street Academy in New York City, it is recommended that the program be continued for the seventh year despite the poorer than expected student gains in all studied components and the sporadic student attendance pattern and high dropout rate. Students…

  11. Legends of the Academy

    ERIC Educational Resources Information Center

    Hernandez, Arelis

    2009-01-01

    For this special edition on "Higher Education Careers," "Diverse" caught up with some legends whose careers have made an indelible impact on the academy. "Diverse" found that even in retirement, or semi-retirement, these pioneers never venture far from the passions that made them great educators and diversity advocates. This article features four…

  12. The Teen Parent Academy

    ERIC Educational Resources Information Center

    Baptiste, H. Prentice, Jr.; Walker, Diane

    2005-01-01

    Pregnant teenagers and young parents often do not receive the quality of education available to other students. Most schools do not have a separate facility or program that deals with their special needs. Pregnant teens and teen parents should not be left behind. The Teen Parent Academy--a unique program in a predominantly Hispanic Texas border…

  13. Peninsula Academies Program.

    ERIC Educational Resources Information Center

    Sequoia Union High School District, Redwood City, CA.

    THE FOLLOWING IS THE FULL TEXT OF THIS DOCUMENT: The Peninsula Academies program helps educationally disadvantaged youth overcome the handicaps of low academic achievement, lack of skills, and chronic unemployment. This is accomplished by providing a high school curriculum that is clearly related to work, training in specific job skills, emphasis…

  14. The California Partnership Academies.

    ERIC Educational Resources Information Center

    Raby, Marilyn

    The California Partnership Academies Program is a highly successful school/business collaboration that allows students who are at risk of not graduating from high school to see clearly the connection between school and the workplace. The following key components are discussed: (1) an at-risk student population made up largely of the educationally…

  15. Dual Enrollment Academy Programs

    ERIC Educational Resources Information Center

    Gonzalez, Nicolas; Chavez, Guadalupe

    2009-01-01

    Dual Enrollment Engineering (DEEA) and Medical Science (DEMSA) Academies are two-year dual enrollment programs for high school students. Students explore engineering and medical careers through college coursework. Students prepare for higher education in engineering and medical fields while completing associate degrees in biology or engineering…

  16. The Global Youth Academy.

    ERIC Educational Resources Information Center

    Alschuler, Alfred; Myers, Stephen

    1995-01-01

    The Global Youth Academy began as a low-cost, private school that taught global citizenship to its own students and foreign students recruited enroute. The school-on-wings formula is simple: build the curriculum into a tour, integrate academic and personal growth, and stress community service. The program succeeds academically while promoting…

  17. Valor Collegiate Academies

    ERIC Educational Resources Information Center

    EDUCAUSE, 2015

    2015-01-01

    The four guiding principles behind the blended, competency-based, personalized learning model of Valor Collegiate Academies, a charter organization serving grades 5-12 in Nashville, TN: (1) Reflect the diversity of both our country and local community; (2) Personalize a student's experience to meet his/her unique academic and non-academic needs;…

  18. Academy Engages Incarcerated Youths

    ERIC Educational Resources Information Center

    Zehr, Mary Ann

    2010-01-01

    It's not easy to keep young people on task for learning in a youth prison, but David Domenici, the principal of the Maya Angelou Academy, a charter-like school serving incarcerated juveniles, is trying to do it while at the same time creating a model program for improving educational services for young offenders. Located at the New Beginnings…

  19. Free Schools and Academies

    ERIC Educational Resources Information Center

    Eades, Jane

    2014-01-01

    The number and range of problems associated with academies and "free" schools is worrying and can be attributed to the lack of ability of central Government to oversee the increasingly fragmented education provision. In this article, the author looks at just a few of those problems which have been reported since she started to log them…

  20. Let's Look at Academies Systemically

    ERIC Educational Resources Information Center

    Glatter, Ron

    2009-01-01

    In this article the author seeks to look at the policy on Academies from a broader perspective, briefly touching on issues of democracy, autonomy, governance and accountability and arguing that the policy must be considered holistically. Individuals should focus not on an Academy or Academies in isolation but on their relationship with the rest of…

  1. Nap sleep preserves associative but not item memory performance.

    PubMed

    Studte, Sara; Bridger, Emma; Mecklinger, Axel

    2015-04-01

    Many studies have shown that sleep improves memory performance, and that even short naps during the day are beneficial. Certain physiological components of sleep such as spindles and slow-wave-sleep are thought to be particularly important for memory consolidation. The aim of this experiment was to reveal the role of naps for hippocampus-dependent associative memory (AM) and hippocampus-independent item memory (IM) alongside their corresponding ERP old/new effects. Participants learnt single words and word-pairs before performing an IM- and an AM-test (baseline). One group was subsequently allowed to nap (∼90min) while the other watched DVDs (control group). Afterwards, both groups performed a final IM- and AM-test for the learned stimuli (posttest). IM performance decreased for both groups, while AM performance decreased for the control group but remained constant for the nap group, consistent with predictions concerning the selective impact of napping on hippocampus-dependent recognition. Putative ERP correlates of familiarity and recollection were observed in the IM posttest, whereas only the later recollection-related effect was present in the AM test. Notably, none of these effects varied with group. Positive correlations were observed between spindle density during slow-wave-sleep and AM posttest performance as well as between spindle density during non-REM sleep and AM baseline performance, showing that successful learning and retrieval both before and after sleep relates to spindle density during nap sleep. Together, these results speak for a selective beneficial impact of naps on hippocampus-dependent memories.

  2. Interaction of NAP-22 with brain glutamic acid decarboxylase (GAD).

    PubMed

    Maekawa, Shohei; Kobayashi, Yuumi; Odagaki, Sin-Ichi; Makino, Midori; Kumanogoh, Haruko; Nakamura, Shun; Morita, Mitsuhiro; Hayashi, Fumio

    2013-03-14

    NAP-22 (also called BASP1 or CAP-23) is a neuron-enriched protein localized mainly in the synaptic vesicles and the synaptic plasma membrane. Biochemically, it is recovered in the lipid raft fraction. In order to understand the physiological function of the neuronal lipid raft, NAP-22 binding proteins were screened with a pull-down assay. Glutamic acid decarboxylase (GAD) was detected through LC-MS/MS, and Western blotting using a specific antibody confirmed the result. Two isoforms of GAD, GAD65 and GAD67, were expressed in bacteria as GST-fusion forms and the interaction with NAP-22 was confirmed in vitro. Partial co-localization of NAP-22 with GAD65 and GAD67 was also observed in cultured neurons. The binding showed no effect on the enzymatic activity of GAD65 and GAD67. These results hence suggest that NAP-22 could participate in the transport of GAD65 and GAD67 to the presynaptic termini and their retention on the synaptic vesicles as an anchoring protein.

  3. Molecular evolution and species-specific expansion of the NAP members in plants.

    PubMed

    Fan, Kai; Shen, Hao; Bibi, Noreen; Li, Feng; Yuan, Shuna; Wang, Ming; Wang, Xuede

    2015-08-01

    The NAP (NAC-Like, Activated by AP3 /PI) subfamily is one of the important plant-specific transcription factors, and controls many vital biological processes in plants. In the current study, 197 NAP proteins were identified from 31 vascular plants, but no NAP members were found in eight non-vascular plants. All NAP proteins were phylogenetically classified into two groups (NAP I and NAP II), and the origin time of the NAP I group might be relatively later than that of the NAP II group. Furthermore, species-specific gene duplications, caused by segmental duplication events, resulted in the expansion of the NAP subfamily after species-divergence. Different groups have different expansion rates, and the NAP group preference was found during the expansion in plants. Moreover, the expansion of NAP proteins may be related to the gain and loss of introns. Besides, functional divergence was limited after the gene duplication. Abscisic acid (ABA) might play an important role in leaf senescence, which is regulated by NAP subfamily. These results could lay an important foundation for expansion and evolutionary analysis of NAP subfamily in plants.

  4. Sonochemical synthesis of zeolite NaP from clinoptilolite.

    PubMed

    Behin, Jamshid; Kazemian, Hossein; Rohani, Sohrab

    2016-01-01

    In the present work, natural clinoptilolite was converted to zeolite NaP using ultrasonic energy, in which the transformation time shortened remarkably. The effect of post-synthesis treatment using conventional hydrothermal was also investigated. The synthesized powders were characterized by XRD, TGA/DTA, SEM, and PSD analysis. The results showed that, increasing the sonication time (energy) has no significant effect on the product's morphology. The crystallinity of the synthesized samples increased slightly with increasing sonication time, but their yield remained relatively unchanged. Furthermore, post-synthesis hydrothermal treatment showed very little influence on properties of the final product. Because the ultrasonic irradiation creates acoustic cavitation cracks on the surface structure of clinoptilolite particulates and increases the concentration of soluble alumino-silicate species, which favors the prevailing super-saturation, crystallization and crystal growth of zeolite NaP happen faster. The particles of zeolite NaP synthesized by ultrasonic irradiation consist of small crystallites of uniform size.

  5. Use of NAP gene to manipulate leaf senescence in plants

    DOEpatents

    Gan, Susheng; Guo, Yongfeng

    2013-04-16

    The present invention discloses transgenic plants having an altered level of NAP protein compared to that of a non-transgenic plant, where the transgenic plants display an altered leaf senescence phenotype relative to a non-transgenic plant, as well as mutant plants comprising an inactivated NAP gene, where mutant plants display a delayed leaf senescence phenotype compared to that of a non-mutant plant. The present invention also discloses methods for delaying leaf senescence in a plant, as well as methods of making a mutant plant having a decreased level of NAP protein compared to that of a non-mutant plant, where the mutant plant displays a delayed leaf senescence phenotype relative to a non-mutant plant. Methods for causing precocious leaf senescence or promoting leaf senescence in a plant are also disclosed. Also disclosed are methods of identifying a candidate plant suitable for breeding that displays a delayed leaf senescence and/or enhanced yield phenotype.

  6. Napping Promotes Inter-Session Habituation to Emotional Stimuli

    PubMed Central

    Pace-Schott, Edward F.; Shepherd, Elizabeth; Spencer, Rebecca M.C.; Marcello, Matthew; Tucker, Matthew; Propper, Ruth E.; Stickgold, Robert

    2010-01-01

    The effects of a daytime nap on inter-session habituation to aversive visual stimuli were investigated. Healthy young adult volunteers viewed repeated presentations of highly negative and emotionally neutral (but equally arousing) International Affective Picture System (IAPS) photographs during two afternoon sessions separated by 2.5 hrs. Half of the photographs were shown at both sessions (Repeated Sets) and half differed between sessions (Novel Sets). For each stimulus presentation, evoked skin conductance response (SCR), heart rate deceleration (HRD) and corrugator supercilii EMG response (EMG), were computed and range corrected using respective maximum session-1 responses. Following each presentation, subjects rated each photograph on dimensions of pleasantness and arousability. During the inter-session interval, Nap subjects had a 120-min polysomnographically monitored sleep opportunity, whereas Wake subjects watched a non-stimulating video. Nap and Wake subjects did not differ in their subjective ratings of photographs. However, for Repeated-Set photographs, Nap subjects demonstrated greater inter-session habituation in SCR and EMG but a trend toward lesser inter-session habituation in HRD. These group differences were absent for Novel-Set photographs. Group differences across all measures were greater for negative stimuli. Occurrence of SWS during the nap was associated with greater inter-session habituation of EMG whereas occurrence of REM was associated with lesser inter-session habituation of SCR to negative stimuli. Sleep may therefore promote emotional adjustment at the level of somatic responses. Physiological but not subjective inter-session habituation to aversive images was enhanced by a daytime nap. PMID:20969968

  7. National Academy Study on the Comprehensive Nuclear Test Ban Treaty

    NASA Astrophysics Data System (ADS)

    Hafemeister, David

    2004-03-01

    After the 51-48 defeat of the CTBT in the Senate, General John Schalikashvili (Former Chair, Joint Chiefs of Staff) commissioned the National Academy of Sciences to examine the following technical issues, which will be summarized*: (1) US Capacity to maintain safety/reliability and design/evaluation of its nuclear stockpile without testing. (2) International/US capability to monitor a nuclear test ban, including evasion scenarios. (3) Ability of nations to increase nuclear capability with/without cheating and the potential effect on of cheating on US security * Technical Issues Related to the Comprehensive Nuclear Test Ban Treaty, National Academy Press, 2003. D. Hafemeister, Physics of Societal Issues, Springer-Verlag/AIP Press, 2004.

  8. Hour-Long Nap May Boost Brain Function in Older Adults

    MedlinePlus

    ... fullstory_162923.html Hour-Long Nap May Boost Brain Function in Older Adults Linked to improved memory and ... during the day had any effects on their brain function. Nearly 60 percent of the people regularly napped ...

  9. Effects of physical positions on sleep architectures and post-nap functions among habitual nappers.

    PubMed

    Zhao, Dayong; Zhang, Qinglin; Fu, Mingqiu; Tang, Yunglung; Zhao, Yufang

    2010-03-01

    The post-lunch sleepiness is considered to be part of biological rhythm. Many people take nap as a countermeasure to this afternoon circadian nadir. The aim of the study is to investigate the effect of a short-term midday nap in different physical positions after a full night sleep on subjective and physiological measures. Thirty-six young healthy habitual nappers (18 men, 18 women) aged 18-23 participated in the study. They were randomly assigned to three experimental conditions: to nap in a seat (NS), to nap in a bed (NB) and no-nap (NN). Measures of subjective sleepiness, mood, fatigue, and P300 component were taken before and after a 20min nap. Results showed that sleepiness, fatigue, and mood for both NS and NB were improved after napping. Moreover, objective alertness was enhanced in NB relative to NS and NN, which showed the larger P300 amplitude after nap. The delta power during sleep stage 2 of NS was inferior to that of NB, which induced a lower alertness after the sitting nap. Consequently, the result demonstrated the function of delta wave during stage 2 on arousal level following the brief midday nap. In general, taking a nap in a seat is a way to subjective restoration but taking a nap in a bed benefits the habitual nappers subjectively and physiologically.

  10. Taking a nap is the new white-knuckle ride.

    PubMed

    Bates, Jane

    2016-09-07

    I awoke from my afternoon slumber, stretched like a cat on a warm pillow, slept some more, then opened my eyes and contemplated the sheer joy of an afternoon nap in summer, lulled to sleep by the sun and the sound of bees buzzing and doves cooing….

  11. 8. VIEW SHOWING UNDERSIDE OF BRIDGE BETWEEN NAPPING BUILDING AND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. VIEW SHOWING UNDERSIDE OF BRIDGE BETWEEN NAPPING BUILDING AND CLOTH ROOM, INCLUDING STEEL LEGS, STRUCTURAL CONFIGURATION OF CONCRETE BRIDGE FLOOR, AND CANTED CONNECTION WITH THIRD STORY OF CLOTH ROOM, LOOKING NORTH - Massachusetts Mills, Cloth Room-Section 15, 95 Bridge Street, Lowell, Middlesex County, MA

  12. 6. VIEW OF SOUTHEAST ELEVATION, SHOWING BRIDGE TO NAPPING BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. VIEW OF SOUTHEAST ELEVATION, SHOWING BRIDGE TO NAPPING BUILDING AT LEFT, OVERHEAD; AND ALTERATIONS OF FENESTRATION FOR TWO FREIGHT DELIVERY DOORS, LOOKING NORTH. DOUBLE DOOR AT LEFT SERVES SOUTH STAIR TOWER - Massachusetts Mills, Cloth Room-Section 15, 95 Bridge Street, Lowell, Middlesex County, MA

  13. Delayed benefit of naps on motor learning in preschool children

    PubMed Central

    Desrochers, Phillip C.; Kurdziel, Laura B. F.; Spencer, Rebecca M. C.

    2015-01-01

    Sleep benefits memory consolidation across a variety of domains in young adults. However, while declarative memories benefit from sleep in young children, such improvements are not consistently seen for procedural skill learning. Here we examined whether performance improvements on a procedural task, although not immediately observed, are evident after a longer delay when augmented by overnight sleep (24-hrs after learning). We trained forty-seven children, aged 33–71 months, on a serial reaction time task and, using a within-subject design, evaluated performance at three time points: immediately after learning, after a daytime nap (nap condition) or equivalent wake opportunity (wake condition), and 24-hours after learning. Consistent with previous studies, performance improvements following the nap did not differ from performance improvements following an equivalent interval spent awake. However, significant benefits of the nap were found when performance was assessed 24-hrs after learning. This research demonstrates that motor skill learning is benefited by sleep, but that this benefit is only evident after an extended period of time. PMID:26645305

  14. Delayed benefit of naps on motor learning in preschool children.

    PubMed

    Desrochers, Phillip C; Kurdziel, Laura B F; Spencer, Rebecca M C

    2016-03-01

    Sleep benefits memory consolidation across a variety of domains in young adults. However, while declarative memories benefit from sleep in young children, such improvements are not consistently seen for procedural skill learning. Here we examined whether performance improvements on a procedural task, although not immediately observed, are evident after a longer delay when augmented by overnight sleep (24 h after learning). We trained 47 children, aged 33-71 months, on a serial reaction time task and, using a within-subject design, evaluated performance at three time points: immediately after learning, after a daytime nap (nap condition) or equivalent wake opportunity (wake condition), and 24 h after learning. Consistent with previous studies, performance improvements following the nap did not differ from performance improvements following an equivalent interval spent awake. However, significant benefits of the nap were found when performance was assessed 24 h after learning. This research demonstrates that motor skill learning is benefited by sleep, but that this benefit is only evident after an extended period of time.

  15. Nap Schedules and Sleep Practices in Infant-Toddler Groups.

    ERIC Educational Resources Information Center

    Siren-Tiusanen, Helena; Robinson, Helja Antola

    2001-01-01

    Analyzed sleep-wake schedules and nap times in multiage infant-toddler groups through three case studies in Finnish day care centers. Found complex interactions among family daily patterns, day care patterns, and young children's sleep disturbances. Identified major differences in day care practices regarding sleep quality related to timing,…

  16. Evaluation of Napped Fabrics for Aerosolized Chemical Agent Protection

    DTIC Science & Technology

    1992-01-30

    agents. Based on laboratory testing, napping had little or no effect on the filtration efficiency, physical or insulation properties of the fabrics tested...0.0 ... 0. . 13 VI. RHsical Properties - Fabric . ........................ 15 VII. Physical Properties - Fabric E...material’ s thermal insulation and physical properties. Small scale liquid aerosol fabric swatch testing for filtration efficiency data was conducted on

  17. Types of Primary Insomnia: Is Hyperarousal Also Present during Napping?

    PubMed Central

    Pérusse, Alexandra D.; Turcotte, Isabelle; St-Jean, Geneviève; Ellis, Jason; Hudon, Carol; Bastien, Célyne H.

    2013-01-01

    Study Objectives: The objective of this study was to identify if hyperarousal is a 24-hour phenomenon in insomnia by comparing sleep during napping between good sleepers (GS) and Insomnia sufferers (INS) (subdivided into paradoxical “PARA-I” and psychophysiological “PSY-I”) following a mentally challenging battery of cognitive tests. Design: Cross-sectional comparisons of GS, PSY-I, and PARA-I. Setting: Participants slept for 4 consecutive nights in the laboratory where PSG was recorded. Upon awakening on mornings 2 and 3, cognitive testing (lasting 90-120 min) was administered, followed by a 20-minute nap. Participants: Fourteen PSY-I, 12 PARA-I, and 23 GS completed the study, comprising home questionnaires, clinical interviews, night PSG recordings, cognitive testing, and nap PSG recordings. All participants were between 25 and 50 years of age and met inclusion criteria for PSY-I, PARA-I, or GS. Interventions: N/A. Measurements and Results: On objective nap parameters, GS had a longer total sleep time (TST; p = 0.008) and better sleep efficiency (SE; p = 0.009), than PSY-I and PARA-I, and both groups of INS were awake significantly longer than GS (p = 0.003). Also, PARA-I took significantly more time than GS to fall asleep (p = 0.014). Subjectively reported sleepiness was comparable across the three groups. Positive relationships were observed between SE over the night and SE over the nap the following day. Conclusions: Results show that GS sleep better than INS during naps following prolonged cognitive testing, suggesting that, in INS, hyperarousal predominates over mental fatigue resulting from these tests. These results may parallel what is observed at night when INS experience increased cognitive load but are unable to fall asleep. Citation: Pérusse AD; Turcotte I; St-Jean G; Ellis J; Hudon C; Bastien CH. Types of primary insomnia: is hyperarousal also present during napping? J Clin Sleep Med 2013;9(12):1273-1280. PMID:24340289

  18. Study of NAP adsorption and assembly on the surface of HOPG.

    PubMed

    Korolkov, Vladimir V; Allen, Stephanie; Roberts, Clive J; Gozes, Illana; Tendler, Saul J B

    2014-12-01

    NAP is an octapeptide that has demonstrated a neuroprotective/therapeutic efficacy at very low concentrations in preclinical studies and in a number of clinical trials. Yet little is known about its structural organization at low concentrations. Here, we have employed atomic force microscopy to investigate NAP peptide assembly on graphite in aqueous media at nanomolar concentration. High spatial resolution scans of NAP assemblies reveal their fine structure with clearly resolved single NAP units. This observation leads us to conclude that NAP molecules do not form complex self-assembled structures at nanomolar concentration when adsorbed on graphite surface.

  19. Reciprocating pellet press

    DOEpatents

    Jones, Charles W.

    1981-04-07

    A machine for pressing loose powder into pellets using a series of reciprocating motions has an interchangeable punch and die as its only accurately machines parts. The machine reciprocates horizontally between powder receiving and pressing positions. It reciprocates vertically to press, strip and release a pellet.

  20. The Global University Press

    ERIC Educational Resources Information Center

    Dougherty, Peter J.

    2012-01-01

    The modern world's understanding of American university press has long been shaped by university-press books. American university-press books are good international advertisements for the universities whose logos grace their spines. The growth of transnational scholarship and the expansion of digital communications networks are converging in ways…

  1. Education at the National Academies

    ERIC Educational Resources Information Center

    Labov, Jay B.

    2003-01-01

    The past three issues of "Cell Biology Education" (CBE) have provided overviews of education projects within the National Research Council's (NRC's) Center for Education, Board on Life Sciences, National Academy of Engineering, and National Academy of Science's Office of Public Understanding of Science. In this article, the author provides…

  2. Planning Guide for Career Academies

    ERIC Educational Resources Information Center

    Dayton, Charles

    2010-01-01

    A career academy is a small learning community within a high school, which selects a subset of students and teachers for a two-, three-, or four-year period. Students enter through a voluntary process; they must apply and be accepted, with parental knowledge and support. A career academy involves teachers from different subjects working together…

  3. Self-reported napping and nocturnal sleep in Taiwanese elderly insomniacs.

    PubMed

    Lai, Hui-Ling

    2005-01-01

    Abstract The aim of this study was to investigate the relationships between self-reported nocturnal sleep quality and napping patterns in elderly persons with insomnia and to compare the nocturnal sleep quality between napping and non-napping groups. Convenience sampling was used to recruit 60 community-dwelling elderly residents of Taichung City, Taiwan (age range 60-83 years, mean 67.1 years) who reported insomnia. All participants scored greater than 5 on the Pittsburgh Sleep Quality Index (PSQI) questionnaire. Napping prevalence, frequency, and duration were assessed by participant interview. Self-reported sleep quality, sleep latency, sleep duration, sleep efficiency, sleep disturbance, use of sleep medication, and daytime dysfunction were measured with the PSQI. Sixty-four percentage of participants (n = 38) reported napping. There were no age, gender, and ethnicity differences on napping patterns. Global sleep quality, sleep efficiency, and sleep disturbance were significantly associated with prevalence of napping (r = 0.24-0.26, p < 0.05). A significant correlation was also found between global sleep quality and nap duration (r = 0.31, p < 0.05). Elders in the napping group reported better global sleep quality (t = 2.2, p < 0.05) and sleep efficiency (t = 2.1, p < 0.05) than those in the non-napping group. The findings suggest that there is no need for health care providers to restrict elderly insomniacs' daytime napping.

  4. Resolution of two native monomeric 90 kDa nitrate reductase active proteins from Shewanella gelidimarina and the sequence of two napA genes

    SciTech Connect

    Simpson, Philippa J.L.; McKinzie, Audra A.; Codd, Rachel

    2010-07-16

    Research highlights: {yields} Two monomeric 90 kDa nitrate reductase active proteins from Shewanella gelidimarina. {yields} Sequence of napA from napEDABC-type operon and napA from NapDAGHB-type operon. {yields} Isolation of NAP as NapA or NapAB correlated with NapA P47E amino acid substitution. -- Abstract: The reduction of nitrate to nitrite in the bacterial periplasm occurs in the 90 kDa NapA subunit of the periplasmic nitrate reductase (NAP) system. Most Shewanella genomes contain two nap operons: napEDABC and napDAGHB, which is an unusual feature of this genus. Two native, monomeric, 90 kDa nitrate reductase active proteins were resolved by hydrophobic interaction chromatography from aerobic cultures of Shewanella gelidimarina replete with reduced nitrogen compounds. The 90 kDa protein obtained in higher yield was characterized as NapA by electronic absorption and electron paramagnetic resonance spectroscopies and was identified by LC/MS/MS and MALDI-TOF/TOF MS as NapA from the napEDABC-type operon. The other 90 kDa protein, which was unstable and produced in low yields, was posited as NapA from the napDAGHB-type operon. Two napA genes have been sequenced from the napEDABC-type and napDAGHB-type operons of S. gelidimarina. Native NAP from S. putrefaciens was resolved as one NapA monomer and one NapAB heterodimer. Two amino acid substitutions in NapA correlated with the isolation of NAP as a NapA monomer or a NapAB heterodimer. The resolution of native, redox-active NapA isoforms in Shewanella provides new insight into the respiratory versatility of this genus, which has implications in bioremediation and the assembly of microbial fuel cells.

  5. Dual Roles of Helicobacter pylori NapA in inducing and combating oxidative stress.

    PubMed

    Wang, Ge; Hong, Yang; Olczak, Adriana; Maier, Susan E; Maier, Robert J

    2006-12-01

    Neutrophil-activating protein (NapA) has been well documented to play roles in human neutrophil recruitment and in stimulating host cell production of reactive oxygen intermediates (ROI). A separate role for NapA in combating oxidative stress within H. pylori was implied by studies of various H. pylori mutant strains. Here, physiological analysis of a napA strain was the approach used to assess the iron-sequestering and stress resistance roles of NapA, its role in preventing oxidative DNA damage, and its importance to mouse colonization. The napA strain was more sensitive to oxidative stress reagents and to oxygen, and it contained fourfold more intracellular free iron and more damaged DNA than the parent strain. Pure, iron-loaded NapA bound to DNA, but native NapA did not, presumably linking iron levels sensed by NapA to DNA damage protection. Despite its in vitro phenotype of sensitivity to oxidative stress, the napA strain showed normal (like that of the wild type) mouse colonization efficiency in the conventional in vivo assay. By use of a modified mouse inoculation protocol whereby nonviable H. pylori is first inoculated into mice, followed by (live) bacterial strain administration, an in vivo role for NapA in colonization efficiency could be demonstrated. NapA is the critical component responsible for inducing host-mediated ROI production, thus inhibiting colonization by the napA strain. An animal colonization experiment with a mixed-strain infection protocol further demonstrated that the napA strain has significantly decreased ability to survive when competing with the wild type. H. pylori NapA has unique and separate roles in gastric pathogenesis.

  6. Differential regulation of cyclooxygenase-2 expression by phytosphingosine derivatives, NAPS and TAPS, and its role in the NAPS or TAPS-mediated apoptosis.

    PubMed

    Kim, Hye Jung; Shin, Weonhye; Park, Chang Seo; Kim, Hyung-Ok; Kim, Tae-Yoon

    2003-11-01

    We investigated the effect of novel phytosphingosine derivatives, N-acetyl phytosphingosine (NAPS) and tetra-acetyl phytosphingosine (TAPS), on induction of apoptosis in HaCaT cells in comparison with C2-ceramide. NAPS/TAPS effectively decreased cell viability in a dose dependent manner mainly due to apoptosis. An apoptosis expression array analysis showed that in the TAPS treated cells 13 genes including COX-2 encoding cyclooxygenase-2, the most induced by TAPS, were up-regulated while 23 others down-regulated. Therefore, we examined the mechanism underlying the altered expression of COX-2. Assays with inhibitors and antibodies against proteins involved in signal transduction demonstrated that NAPS and TAPS elevated COX-2 expression via tyrosine kinase, src, PI-3 kinase and PKC, followed by ERK activation. However, P38 was not involved in the NAPS-mediated COX-2 expression but in the TAPS-mediated. We further demonstrated by FACS analyses that NAPS- or TAPS-mediated apoptosis was greatly increased in cells treated with celecoxib, a selective COX-2 inhibitor. Inhibition of the ERK pathway apparently involved in the NAPS/TAPS-mediated COX-2 expression enhanced the NAPS/TAPS-mediated apoptosis, whereas inhibition of the P38 pathway did not. These results suggest that expression of COX-2 in the TAPS- or NAPS-treated cells may be increased to counteract the effect of those compounds on apoptosis.

  7. The utility of a 5th nap in multiple sleep latency test

    PubMed Central

    Lykouras, Dimosthenis; Rees, Kate

    2016-01-01

    Background This is the first study that aimed to look specifically at the utility of the 5th nap in the multiple sleep latency test (MSLT), a test used to assist in the diagnosis of narcolepsy. Methods Data was retrospectively collected from the Sleep Disorders Centre of a Tertiary Hospital on patients that had a 5th nap during their MSLT from the 08th November 2011 to 12th November 2014. Results Fifty-three patients had a 5th nap performed out of 378 MSLT studies. In 16% of cases a diagnosis of narcolepsy was given directly due to the inclusion of the 5th nap on the MSLT. Here a 5th nap allowed diagnostic criteria of mean sleep latency <8 minutes and >2 SOREMPS to be met. In 53% of cases the mean sleep latency increased due to 5th nap inclusion; the mean sleep latency of the first four naps was 5.6 vs. 6.7 after inclusion of the 5th nap. Conclusions The 5th nap is not often performed within the MSLT studies. Our study shows that only a few patients may benefit from a 5th nap opportunity which also led to increase of the mean sleep latency at the expense of extra time, cost, labour and increased patient anxiety. PMID:26904269

  8. Intranasal Delivery of Recombinant NT4-NAP/AAV Exerts Potential Antidepressant Effect.

    PubMed

    Ma, Xian-Cang; Chu, Zheng; Zhang, Xiao-Ling; Jiang, Wen-Hui; Jia, Min; Dang, Yong-Hui; Gao, Cheng-Ge

    2016-06-01

    The present study was designed to construct a recombinant adeno-associated virus (rAAV) which can express NAP in the brain and examine whether this virus can produce antidepressant effects on C57 BL/6 mice that had been subjected to open field test and forced swimming test, via nose-to-brain pathway. When the recombinant plasmid pGEM-T Easy/NT4-NAP was digested by EcoRI, 297 bp fragments can be obtained and NT4-NAP sequence was consistent with the designed sequence confirmed by DNA sequencing. When the recombinant plasmid pSSCMV/NT4-NAP was digested by EcoRI, 297 bp fragments is visible. Immunohistochemical staining of fibroblasts revealed that expression of NAP was detected in NT4-NAP/AAV group. Intranasal delivery of NT4-NAP/AAV significantly reduced immobility time when the FST was performed after 1 day from the last administration. The effects observed in the FST could not be attributed to non-specific increases in activity since intranasal delivery of NT4-NAP/AAV did not alter the behavior of the mice during the open field test. The results indicated that a recombinant AAV vector which could express NAP in cells was successfully constructed and NAP may be a potential target for therapeutic action of antidepressant treatment.

  9. University Presses: Problems and Prospects.

    ERIC Educational Resources Information Center

    Meeker, Robert B.

    Historical information on university presses and their problems are considered. University presses in the United States have their roots in 15th century England when the Oxford University Press was established in 1478. The first U.S. press to use the term "university press" was Cornell University; the press operated from 1869 until it…

  10. Post-sleep inertia performance benefits of longer naps in simulated nightwork and extended operations.

    PubMed

    Mulrine, Hannah M; Signal, T Leigh; van den Berg, Margo J; Gander, Philippa H

    2012-11-01

    Operational settings involving shiftwork or extended operations require periods of prolonged wakefulness, which in conjunction with sleep loss and circadian factors, can have a negative impact on performance, alertness, and workplace safety. Napping has been shown to improve performance and alertness after periods of prolonged wakefulness and sleep loss. Longer naps may not only result in longer-lasting benefits but also increase the risk of sleep inertia immediately upon waking. The time course of performance after naps of differing durations is thus an important consideration in weighing the benefits and risks of napping in workplace settings. The objective of this study was to evaluate the effectiveness of nap opportunities of 20, 40, or 60 min for maintaining alertness and performance 1.5-6 h post-nap in simulated nightwork (P1) or extended operations (P2). Each protocol included 12 participants in a within-subjects design in a controlled laboratory environment. After a baseline 8 h time-in-bed, healthy young males (P1 mean age 25.1 yr; P2 mean age 23.2 yr) underwent either ≈ 20 h (P1) or ≈ 30 h (P2) of sleep deprivation on four separate occasions, followed by nap opportunities of 0, 20, 40, and 60 min. Sleep on the baseline night and during the naps was recorded polysomnographically. During the nap opportunities, sleep onset latency was short and sleep efficiency was high. A greater proportion of slow-wave sleep (SWS) was obtained in nap opportunities of 40 and 60 min compared with 20 min. Rapid eye movement (REM) sleep occurred infrequently. A subjective sleepiness rating (Karolinska Sleepiness Scale, KSS), 2-Back Working Memory Task (WMT), and Psychomotor Vigilance Task (PVT) were completed 1.5, 2, 2.5, 3, 4, 5, and 6 h post-nap. The slowest 10% of PVT responses were significantly faster after 40 and 60 min naps compared with a 20 min (P1) or no (P2) nap. There were significantly fewer PVT lapses after 40 and 60 min naps compared with no nap (P2), and

  11. Sustainable Materials Management Web Academy

    EPA Pesticide Factsheets

    The Sustainable Materials Management (SMM) Web Academy series is a free resource for SMM challenge participants, stakeholders, and anyone else interested in learning more about SMM principles from experts in the field.

  12. American Academy of Forensic Sciences

    MedlinePlus

    ... University Listings FEPAC Accredited Programs Courses in Forensic Odontology Choosing a Career What is Forensic Science? What ... Corner Forensic Sciences Foundation American Society of Forensic Odontology Research Grants Academy Standards Board (ASB) Account Portal ...

  13. The Peninsula Academies. Third Yearly Interim Report.

    ERIC Educational Resources Information Center

    Reynolds, Dorothy F.

    The performance of the Peninsula Academies of Electronics and Computer Technology was assessed for the program's third year of operation in two host schools in the Sequoia Union High School District. Data were collected for approximately 190 Academy students and for a comparison group of non-Academy students. Academies students missed fewer days…

  14. Utah Principals Academy, 1987-1988.

    ERIC Educational Resources Information Center

    Utah State Board of Education, Salt Lake City.

    Improving instructional leadership skills of principals is the focus of the academy. Following a foreword and mission statement by James R. Moss, the state superintendent of public instruction, the booklet describes three programs that help to achieve the academy's goals: Academy Fellows, Academy Seminars, and Cluster Grants. Titles and authors of…

  15. 32 CFR 575.1 - Military Academy.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 3 2013-07-01 2013-07-01 false Military Academy. 575.1 Section 575.1 National... MILITARY ACADEMY § 575.1 Military Academy. (a) Organization and administration. (1) The United States Military Academy is under the general direction and supervision of the Department of the Army....

  16. The Greenwood School Press.

    ERIC Educational Resources Information Center

    Lamb, Jane

    1984-01-01

    A turn-of-the-century printing press motivates elementary students to write. Children write, edit, and print their own stories on the Greenwood School Press. This self-supporting enterprise introduces children to various aspects of writing and producing literature. (DF)

  17. Press Relations: Carter's Example.

    ERIC Educational Resources Information Center

    Kindsvatter, Peter S.

    Although different philosophies may motivate presidential-press relations, causing frequent friction or adversity, there is nevertheless a strong degree of cooperation and collaboration between the press and a president of the United States. This interdependence has been particularly evident in recent administrations, and it progresses…

  18. Lyndon Johnson's Press Conferences.

    ERIC Educational Resources Information Center

    Cooper, Stephen

    Because President Lyndon Johnson understood well the publicity value of the American news media, he sought to exploit them. He saw reporters as "torch bearers" for his programs and policies and used the presidential press conference chiefly for promotional purposes. Although he met with reporters often, his press conferences were usually…

  19. High School Press Pressures.

    ERIC Educational Resources Information Center

    Rogers, Luella P.

    History shows that the high school press suffers through cycles that reflect economic factors and cultural climates within communities, states, and the nation. The direction of that cycle in the 1960s and early 1970s was toward more open, free-flowing information by a vigorous student press, but those economic and cultural signs now are pointing…

  20. Minimal Effect of Daytime Napping Behavior on Nocturnal Sleep in Pregnant Women

    PubMed Central

    Ebert, Rebecca M.; Wood, Annette; Okun, Michele L.

    2015-01-01

    Study Objectives: To assess whether daytime naps negatively impact nocturnal sleep. Design: Longitudinal, prospective cohort design. Participants: 161 pregnant women recruited and evaluated in early gestation (10–20 weeks). Measurements and Results: Daily sleep information was collected in three 2-week periods (10–12, 14–16, and 18–20 weeks gestation) with a daily sleep diary and an actigraph. The average number of naps, as well as the average length of each nap, were calculated from sleep diaries. Women were categorized first as non nappers (0 naps/2-week period), moderate nappers (1–3 naps/2-week period), or frequent nappers (≥ 4 naps/2-week period). Then, based on the average nap length, they were categorized as short (< 90 min) or long (≥ 90 min) nappers. Nocturnal sleep parameters included SOL, WASO, SE, and TST. SAS procedure MIXED was used for modeling the main effects of nap group and time, and time by nap group interactions. Women who took naps had a decrease in diary-assessed nocturnal TST, but not actigraphy-assessed TST. This observation was group- and time-specific. There were no other group differences. Women who napped ≥ 90 min had poorer diary-assessed SE and lower diary-assessed TST than those who took shorter naps. Length of nap was not associated with any other sleep measures. Conclusions: The number of daytime naps have minimal impact on nocturnal sleep parameters; however, long nappers did exhibit modestly impaired sleep continuity and sleep quality. Overall, we propose that daytime naps provide a beneficial countermeasure to the sleep disruption commonly reported by pregnant women. This may be clinically beneficial given that sleep continuity and quality are important correlates of pregnancy outcomes. Commentary: A commentary on this article appears in this issue on page 593. Citation: Ebert RM, Wood A, Okun ML. Minimal effect of daytime napping behavior on nocturnal sleep in pregnant women. J Clin Sleep Med 2015;11(6):635

  1. The ADNP derived peptide, NAP modulates the tubulin pool: implication for neurotrophic and neuroprotective activities.

    PubMed

    Oz, Saar; Ivashko-Pachima, Yanina; Gozes, Illana

    2012-01-01

    Microtubules (MTs), key cytoskeletal elements in living cells, are critical for axonal transport, synaptic transmission, and maintenance of neuronal morphology. NAP (NAPVSIPQ) is a neuroprotective peptide derived from the essential activity-dependent neuroprotective protein (ADNP). In Alzheimer's disease models, NAP protects against tauopathy and cognitive decline. Here, we show that NAP treatment significantly affected the alpha tubulin tyrosination cycle in the neuronal differentiation model, rat pheochromocytoma (PC12) and in rat cortical astrocytes. The effect on tubulin tyrosination/detyrosination was coupled to increased MT network area (measured in PC12 cells), which is directly related to neurite outgrowth. Tubulin beta3, a marker for neurite outgrowth/neuronal differentiation significantly increased after NAP treatment. In rat cortical neurons, NAP doubled the area of dynamic MT invasion (Tyr-tubulin) into the neuronal growth cone periphery. NAP was previously shown to protect against zinc-induced MT/neurite destruction and neuronal death, here, in PC12 cells, NAP treatment reversed zinc-decreased tau-tubulin-MT interaction and protected against death. NAP effects on the MT pool, coupled with increased tau engagement on compromised MTs imply an important role in neuronal plasticity, protecting against free tau accumulation leading to tauopathy. With tauopathy representing a major pathological hallmark in Alzheimer's disease and related disorders, the current findings provide a mechanistic basis for further development. NAP (davunetide) is in phase 2/3 clinical trial in progressive supranuclear palsy, a disease presenting MT deficiency and tau pathology.

  2. Biochemical interaction of an actin-capping protein, CapZ, with NAP-22.

    PubMed

    Odagaki, Sin-Ichi; Kumanogoh, Haruko; Nakamura, Shun; Maekawa, Shohei

    2009-07-01

    NAP-22 is a neuronal protein localized in the presynaptic membrane and synaptic vesicles and recovered in a Triton-insoluble low-density microdomain fraction after biochemical fractionation of the synaptic plasma membrane. NAP-22 organizes membrane microdomains through binding to membrane lipids such as cholesterol, phosphatidylethanolamine, and phosphatidylinositol 4,5-bisphosphate. In this study, NAP-22-binding proteins were screened through the pull-down assay using brain-derived NAP-22 bound to Sepharose 4B. An actin-capping protein, CapZ, was identified in the precipitate through mass spectrometry and Western blotting. CapZ was then expressed in E. coli and the purified protein-bound NAP-22 directly. Because bacterially expressed NAP-22 bound CapZ, it was determined that the N-terminal myristoyl moiety of NAP-22 is not necessary for the binding. The binding of NAP-22 showed no effect on the actin nucleation activity of CapZ measured with centrifugation and viscometric assays. Hence, the CapZ-NAP-22 complex could work as the nucleation site of actin polymerization or as the actin filament-anchoring site on the membrane microdomain.

  3. 1997 NASA Academy in Aeronautics

    NASA Technical Reports Server (NTRS)

    Andrisani, Dominick, II

    1998-01-01

    The NASA Academy in Aeronautics at the Dryden Flight Research Center (DFRC) was a ten-week summer leadership training program conducted for the first time in the summer of 1997. Funding was provided by a contract between DFRC and Purdue University. Mr. Lee Duke of DFRC was the contract monitor, and Professor Dominick Andrisani was the principal investigator. Five student research associates participated in the program. Biographies of the research associates are given in Appendix 1. Dominick Andrisani served as Dean of the NASA Academy in Aeronautics. NASA Academy in Aeronautics is a unique summer institute of higher learning that endeavors to provide insight into all of the elements that make NASA aeronautical research possible. At the same time the Academy assigns the research associate to be mentored by one of NASA!s best researchers so that they can contribute towards an active flight research program. Aeronautical research and development are an investment in the future, and NASA Academy is an investment in aeronautical leaders of the future. The Academy was run by the Indiana Space Grant Consortium at Purdue in strategic partnership with the National Space Grant College and Fellowship Program. Research associates at the Academy were selected with help from the Space Grant Consortium that sponsored the research associate. Research associate stipend and travel to DFRC were paid by the students' Space Grant Consortium. All other student expenses were paid by the Academy. Since the Academy at DFRC had only five students the opportunity for individual growth and attention was unique in the country. About 30% of the working time and most of the social time of the students were be spent as a "group" or "team." This time was devoted to exchange of ideas, on forays into the highest levels of decision making, and in executing aeronautical research. This was done by interviewing leaders throughout the aerospace industry, seminars, working dinners, and informal

  4. How do the timing and length of a night-shift nap affect sleep inertia?

    PubMed

    Kubo, Tomohide; Takahashi, Masaya; Takeyama, Hidemaro; Matsumoto, Shun; Ebara, Takeshi; Murata, Kensaburo; Tachi, Norihide; Itani, Toru

    2010-07-01

    Napping is one strategy that may assist night shiftworkers to cope with sleepiness and fatigue. However, one potential disadvantage of napping is that awakening from naps is disturbed by sleep inertia, which has also been found to impair performance and/or mood, transiently. The authors examined the effects of the timing and length of a night-shift nap on sleep inertia in a laboratory setting. Twelve male university students (mean +/- SD: 21.6 +/- 2.8 yrs) participated in this 3-day experiment, during which included a simulated night shift (22:00-08:00 h) and subsequent day (11:30-17:30 h) and night sleep (00:00-07:00 h). The simulated night shift was designed to include one of five (four nap/one no-nap) conditions. The napping conditions differed by their timing and duration: 00:00-01:00 h (Early 60 min; E60), 00:00-02:00 h (Early 120 min; E120), 04:00-05:00 h (Late 60 min; L60), 04:00-06:00 h (Late 120 min; L120). Participants completed all the experimental conditions in a counterbalanced order. Rectal temperature (R(T)) was recorded throughout the simulated shift and polysomnography (PSG) was recorded during the nap period. Immediately before and after each nap, participants were required to complete a visual analogue scale (VAS) to assess sleepiness and a visual vigilance test (VVT). During the simulated night shift, a set of tasks (an English transcription task, a performance test battery, and a break) was repeated hourly, except during the periods of napping. For each nap condition, the VAS and VVT (reaction time [RT]; lapses >5 s) results were analyzed by two-way, repeated-measures analysis of variance (ANOVA) (nap [nap versus no-nap] x time point [pre-nap versus post-nap]). PSG and R(T) data were analyzed with one-way repeated-measures ANOVA. Marginally significant interactions were observed for RTs and lapses in VVT for the L60 nap condition (p = .071 and p = .070, respectively). However, those effect sizes were moderate (partial eta(2) = 0.266, 0

  5. Simulation of nap-of-the-Earth flight in helicopters

    NASA Technical Reports Server (NTRS)

    Condon, Gregory W.

    1991-01-01

    NASA-Ames along with the U.S. Army has conducted extensive simulation studies of rotorcraft in the nap-of-the-Earth (NOE) environment and has developed facility capabilities specifically designed for this flight regime. The experience gained to date in applying these facilities to the NOE flight regime are reported along with the results of specific experimental studies conducted to understand the influence of both motion and visual scene on the fidelity of NOE simulation. Included are comparisons of results from concurrent piloted simulation and flight research studies. The results of a recent simulation experiment to study simulator sickness in this flight regime is also discussed.

  6. Considerations for automated nap-of-the-earth rotorcraft flight

    NASA Technical Reports Server (NTRS)

    Cheng, Victor H. L.; Sridhar, Banavar

    1988-01-01

    The authors consider nap-of-the-earth (NOE) rotorcraft flight as one of the applications in which obstacle avoidance plays a key role, and investigate the prospects of automating the guidance functions of NOE flight. Based on a proposed structure for the guidance functions, obstacle detection and obstacle avoidance are identified as the two critical components requiring substantial advancement before an automating guidance system can be realized. The major sources of difficulties in developing these two components are discussed, including sensor requirements for which a systematic analysis is provided.

  7. Simulation of nap-of-Earth flight in helicopters

    NASA Technical Reports Server (NTRS)

    Condon, Gregory W.

    1990-01-01

    NASA-Ames in conjunction with U.S. Army has conducted extensive simulation investigations of rotorcraft in the nap-of-the-Earth (NOE) environment and has developed facility capabilities specifically designed for this flight regime. The experience gained to date in applying these facilities to the NOE flight regime are reported along with the results of specific experimental investigations conducted to understand the influence of both motion and visual scene on the fidelity of NOE simulation. Included are comparisons of results from concurrent piloted simulation and flight research investigations. The results of a recent simulation experiment to investigate simulator sickness in this flight regime is also discussed.

  8. Actigraphic assessment of a polysomnographic-recorded nap: a validation study.

    PubMed

    Kanady, Jennifer C; Drummond, Sean P A; Mednick, Sara C

    2011-03-01

    This study aimed to determine if actigraphy could differentiate sleep and wake during a daytime nap and no-nap rest period. Fifty-seven subjects participated in the study; 30 subjects were in the nap group and the remaining 27 in the no-nap comparison group. All subjects wore actigraphs while simultaneously undergoing polysomnography (PSG). Three actigraphic sensitivity levels (high, medium, low) and two interval duration minimums (15 and 40 min) were used to score the nap and no-nap data. The variables examined included total sleep time (TST), sleep latency (SL), wake after sleep onset (WASO) and sleep efficiency (SE). The Bland-Altman technique was used to determine concordance. Epoch-by-epoch analysis examined actigraphic accuracy, sensitivity and specificity. For the naps, all actigraph settings except low-40 showed significant correlations with TST. The high and medium settings predicted SE significantly and the high settings predicted SL significantly. Bland-Altman analyses demonstrated high settings overestimated TST while high and medium settings overestimated SE. Overall, for the nap condition accuracy for the actigraph was 82-86%, sensitivity was 92-96% and specificity was 40-67%. In the no-nap condition, accuracy for the actigraph was 60-84%, sensitivity was 47-78% and specificity was 60-86%. Medium-40 and low-40 were the only settings that did not misidentify sleep in the no-nap condition. These results suggest that actigraphy can predict TST, SE and SL reliably, depending upon parameter settings, and actigraphy is a highly sensitive but not specific measure for daytime naps. Different actigraphy settings may be optimal depending upon the variables of interest. Discrimination of sleep and wake during periods of waking quiescence is not as robust as during periods of mainly daytime sleep.

  9. Crystallization and preliminary X-ray analysis of the periplasmic nitrate reductase (NapA-NapB complex) from Rhodobacter sphaeroides f. sp. denitrificans.

    PubMed

    Pignol, D; Adriano, J M; Fontecilla-Camps, J C; Sabaty, M

    2001-12-01

    The periplasmic nitrate reductase of Rhodobacter sphaeroides f. sp. denitrificans is a heterodimer responsible for the first step of reduction in the denitrification process by the conversion of nitrate to nitrite. It consists of a 91 kDa molybdenum-containing catalytic subunit (NapA) and a 17 kDa dihaem cytochrome c (NapB). Crystals of the NapA-NapB complex were obtained by the vapour-diffusion method using ammonium sulfate as precipitant. They belong to the P6(1)22 space group, with unit-cell parameters a = b = 151.9, c = 255.8 A, and contain a single complex in the asymmetric unit. A complete native data set was collected at a synchrotron source to 3.1 A resolution.

  10. Can a short nap and bright light function as implicit learning and visual search enhancers?

    PubMed

    Kaida, Kosuke; Takeda, Yuji; Tsuzuki, Kazuyo

    2012-01-01

    The present study examined effects of a short nap (20 min) and/or bright light (2000 lux) on visual search and implicit learning in a contextual cueing task. Fifteen participants performed a contextual cueing task twice a day (1200-1330 h and 1430-1600 h) and scored subjective sleepiness before and after a short afternoon nap or a break period. Participants served a total of four experimental conditions (control, short nap, bright light and short nap with bright light). During the second task, bright light treatment (BLT) was applied in the two of the four conditions. Participants performed both tasks in a dimly lit environment except during the light treatment. Results showed that a short nap reduced subjective sleepiness and improved visual search time, but it did not affect implicit learning. Bright light reduced subjective sleepiness. A short nap in the afternoon could be a countermeasure against sleepiness and an enhancer for visual search. Practitioner Summary: The study examined effects of a short afternoon nap (20 min) and/or bright light (2000 lux) on visual search and implicit learning. A short nap is a powerful countermeasure against sleepiness compared to bright light exposure in the afternoon.

  11. The femtomolar-acting NAP interacts with microtubules: Novel aspects of astrocyte protection.

    PubMed

    Gozes, Illana; Divinski, Inna

    2004-12-01

    Activity-dependent neuroprotective protein (ADNP), a gene product essential for brain formation, contains a short octapeptide sequence NAPVSIPQ (NAP) that protects neurons against a wide variety of insults. At the pico-molar concentration range, NAP provides neuroprotection by direct interaction with neurons. At the femtomolar concentration range, NAP requires the presence of glial cells to provide neuroprotection. To further understand the mechanism of neuroprotection afforded by NAP, specific binding proteins were searched for. Tubulin, the major subunit protein of microtubules, was identified as a NAP binding molecule. NAP structure allows membrane penetration, followed by tubulin binding and facilitation of microtubule assembly toward cellular protection in astrocytes. NAP (10(-15) M) promoted microtubule assembly in vitro and protected astrocytes against zinc intoxication which is associated with microtubule disruption. A two hour incubation period of astrocytes with femtomolar concentrations of NAP resulted in microtubule re-organization and transient increases in immunoreactive non-phosphorylated tau. Microtubules are the key component of the neuronal and glial cytoskeleton that regulates cell division, differentiation and protection, while tau pathology is a major contributor to Alzheimer's disease and other dementias. The findings described here may open up new horizons in research and development of neuroprotective compounds.

  12. Effects of afternoon "siesta" naps on sleep, alertness, performance, and circadian rhythms in the elderly

    NASA Technical Reports Server (NTRS)

    Monk, T. H.; Buysse, D. J.; Carrier, J.; Billy, B. D.; Rose, L. R.

    2001-01-01

    STUDY OBJECTIVES: To determine the effects of a 90-minute afternoon nap regimen on nocturnal sleep, circadian rhythms, and evening alertness and performance levels in the healthy elderly. DESIGN AND SETTING: Nine healthy elderly subjects (4m, 5f, age range 74y-87y) each experienced both nap and no-nap conditions in two studies each lasting 17 days (14 at home, 3 in the laboratory). In the nap condition a 90-minute nap was enforced between 13:30 and 15:00 every day, in the no-nap condition daytime napping was prohibited, and activity encouraged in the 13:30-15:00 interval. The order of the two conditions was counterbalanced. PARTICIPANTS: N/A INTERVENTIONS: N/A MEASUREMENTS: Diary measures, pencil and paper alertness tests, and wrist actigraphy were used at home. In the 72 hour laboratory studies, these measures were augmented by polysomnographic sleep recording, continuous rectal temperature measurement, a daily evening single trial of a Multiple Sleep Latency Test (MSLT), and computerized tests of mood, activation and performance efficiency. RESULTS: By the second week in the "at home" study, an average of 58 minutes of sleep was reported per siesta nap; in the laboratory, polysomnography confirmed an average of 57 minutes of sleep per nap. When nap and no-nap conditions were compared, mixed effects on nocturnal sleep were observed. Diary measures indicated no significant difference in nocturnal sleep duration, but a significant increase (of 38 mins.) in 24-hour Total Sleep Time (TST) when nocturnal sleeps and naps were added together (p<0.025). The laboratory study revealed a decrease of 2.4% in nocturnal sleep efficiency in the nap condition (p<0.025), a reduction of nocturnal Total Sleep Time (TST) by 48 mins. in the nap condition (p<0.001) which resulted primarily from significantly earlier waketimes (p<0.005), but no reliable effects on Wake After Sleep Onset (WASO), delta sleep measures, or percent stages 1 & 2. Unlike the diary study, the laboratory study

  13. Serum Helicobacter pylori NapA antibody as a potential biomarker for gastric cancer.

    PubMed

    Liu, Jingjing; Liu, Huimin; Zhang, Tingting; Ren, Xiyun; Nadolny, Christina; Dong, Xiaoqun; Huang, Lina; Yuan, Kexin; Tian, Wenjing; Jia, Yunhe

    2014-02-20

    Helicobacter pylori (H. pylori) infection is strongly associated with gastric cancer. However, only a minority of infected individuals ever develop gastric cancer. This risk stratification may be in part due to differences among strains. The relationship between neutrophil-activating protein (NapA) and gastric cancer is unclear. The purpose of this study is to evaluate the significance of NapA as a biomarker in gastric cancer. We used enzyme linked immunosorbent assay (ELISA) to determine the status of H. pylori infection. Indirect ELISA method was used for detection of NapA antibody titer in the serum of H. pylori infected individuals. Unconditional logistic regressions were adopted to analyze the variables and determine the association of NapA and gastric cancer. The results of study indicated serum H. pylori NapA antibody level were associated with a reduced risk for development of gastric cancer. It may be used in conjugation with other indicators for gastric cancer detection.

  14. Stable complex formation between HIV Rev and the nucleosome assembly protein, NAP1, affects Rev function

    SciTech Connect

    Cochrane, Alan; Murley, Laura Lea; Gao Mian; Wong, Raymond; Clayton, Kiera; Brufatto, Nicole; Canadien, Veronica; Mamelak, Daniel; Chen, Tricia; Richards, Dawn; Zeghouf, Mahel; Greenblatt, Jack; Burks, Christian; Frappier, Lori

    2009-05-25

    The Rev protein of HIV-1 is essential for HIV-1 proliferation due to its role in exporting viral RNA from the nucleus. We used a modified version of tandem affinity purification (TAP) tagging to identify proteins interacting with HIV-1 Rev in human cells and discovered a prominent interaction between Rev and nucleosome assembly protein 1 (Nap1). This interaction was also observed by specific retention of Nap1 from human cell lysates on a Rev affinity column. Nap1 was found to bind Rev through the Rev arginine-rich domain and altered the oligomerization state of Rev in vitro. Overexpression of Nap1 stimulated the ability of Rev to export RNA, reduced the nucleolar localization of Rev, and affected Rev nuclear import rates. The results suggest that Nap-1 may influence Rev function by increasing the availability of Rev.

  15. Reality and dreams: state of the academy.

    PubMed

    Torabi, Mohammad R

    2005-01-01

    These remarks were presented at the opening ceremony of the American Academy of Health Behavior (AAHB) annual scientific conference held in Charleston, SC, in February 2005 by the outgoing president of the Academy.

  16. Internship Handbook for Career Academies.

    ERIC Educational Resources Information Center

    Winthrop, Jerauld

    Career academies are high school programs that frame academic learning around a career focus in order to increase student motivation and achievement. They generally have three defining features: a school-within-a-school structure, a college preparation curriculum with a career theme, and partnerships with employers, the community, and higher…

  17. Carnegie Science Academy Web Site

    NASA Astrophysics Data System (ADS)

    Kotwicki, John; Atzinger, Joe; Turso, Denise

    1997-11-01

    The Carnegie Science Academy is a professional society "For Teens...By Teens" at the Carnegie Science Center in Pittsburgh. The CSA Web Site [ http://csa.clpgh.org ] is designed for teens who have an interest in science and technology. This online or virtual science academy provides resources for teens in high school science classes. The Web site also allows students around the world to participate and communicate with other students, discuss current events in science, share opinions, find answers to questions, or make online friends. Visitors can enjoy the main components of the site or sign up for a free membership which allows access to our chat room for monthly meeting, online newsletter, members forum, and much more. Main components to the site include a spot for cool links and downloads, available for any visitor to download or view. Online exhibits are created by students to examine and publish an area of study and also allow teachers to easily post classroom activities as exhibits by submitting pictures and text. Random Access, the interactive part of the academy, allows users to share ideas and opinions. Planet CSA focuses on current events in science and the academy. In the future the CSA Web site will become a major resource for teens and science teachers providing materials that will allow students to further enhance their interest and experiences in science.

  18. Caliber Schools. Caliber: Beta Academy

    ERIC Educational Resources Information Center

    EDUCAUSE, 2015

    2015-01-01

    Caliber: Beta Academy is reimagining education as we know it, with the belief that the innovations in its model will allow 100% of its students to graduate ready to attend and succeed in a competitive four-year college and beyond. The academic model of the school features personalized learning plans, blended learning for English and math,…

  19. Knowledge Management and the Academy

    ERIC Educational Resources Information Center

    Cain, Timothy J.; Branin, Joseph J.; Sherman, W. Michael

    2008-01-01

    Universities and colleges generate extraordinary quantities of knowledge and innovation, but in many ways the academy struggles to keep pace with the digital revolution. Growing pressures are reshaping how universities must do business--students expecting enhanced access and support, administrators eager to make data-driven strategic decisions,…

  20. Blending Instruction with Khan Academy

    ERIC Educational Resources Information Center

    Cargile, Lori A.

    2015-01-01

    Khan Academy, the free Web-based tutorial program, landed on the education landscape in about 2011, when it was featured on the popular weekly news show "60 Minutes." The program is now used worldwide by more than 10 million learners each month and is used in more than 29,000 classrooms in 216 countries. Are classroom teachers using the…

  1. Adult Learners in the Academy

    ERIC Educational Resources Information Center

    Bash, Lee

    1999-01-01

    Adult learners comprise almost 50 percent of all students enrolled in higher Education. Some argue they are pioneering change in today's higher educational landscape. This book is designed to assist faculty members and administrators who want to understand how the impact of adult learning programs has and is helping to transform the academy and…

  2. Adult Learners in the Academy.

    ERIC Educational Resources Information Center

    Bash, Lee

    Adult learning programs are becoming increasingly important. This book is designed to serve as a wake-up call for members of the academy who prefer to work with traditional students. It provides practical advice for adult learning programs with insights drawn from case studies and the author's experience. Part 1, "Context and Overview," contains:…

  3. INSPIRED High School Computing Academies

    ERIC Educational Resources Information Center

    Doerschuk, Peggy; Liu, Jiangjiang; Mann, Judith

    2011-01-01

    If we are to attract more women and minorities to computing we must engage students at an early age. As part of its mission to increase participation of women and underrepresented minorities in computing, the Increasing Student Participation in Research Development Program (INSPIRED) conducts computing academies for high school students. The…

  4. Effects of sleep inertia after daytime naps vary with executive load and time of day.

    PubMed

    Groeger, John A; Lo, June C Y; Burns, Christopher G; Dijk, Derk-Jan

    2011-04-01

    The effects of executive load on working memory performance during sleep inertia after morning or afternoon naps were assessed using a mixed design with nap/wake as a between-subjects factor and morning/afternoon condition as a within-subject factor. Thirty-two healthy adults (mean 22.5 ± 3.0 years) attended two laboratory sessions after a night of restricted sleep (6 hrs), and at first visit, were randomly assigned to the Nap or Wake group. Working memory (n-back) and subjective workload were assessed approximately 5 and 25 minutes after 90-minute morning and afternoon nap opportunities and at the corresponding times in the Wake condition. Actigraphically assessed nocturnal sleep duration, subjective sleepiness, and psychomotor vigilance performance before daytime assessments did not vary across conditions. Afternoon naps showed shorter EEG assessed sleep latencies, longer sleep duration, and more Slow Wave Sleep than morning naps. Working memory performance deteriorated, and subjective mental workload increased at higher executive loadings. After afternoon naps, participants performed less well on more executive-function intensive working memory tasks (i.e., 3-back), but waking and napping participants performed equally well on simpler tasks. After some 30 minutes of cognitive activity, there were no longer performance differences between the waking and napping groups. Subjective Task Difficulty and Mental Effort requirements were less affected by sleep inertia and dissociated from objective measures when participants had napped in the afternoon. We conclude that executive functions take longer to return to asymptotic performance after sleep than does performance of simpler tasks which are less reliant on executive functions.

  5. A NAP-AAO3 regulatory module promotes chlorophyll degradation via ABA biosynthesis in Arabidopsis leaves.

    PubMed

    Yang, Jiading; Worley, Eric; Udvardi, Michael

    2014-12-01

    Chlorophyll degradation is an important part of leaf senescence, but the underlying regulatory mechanisms are largely unknown. Excised leaves of an Arabidopsis thaliana NAC-LIKE, ACTIVATED BY AP3/PI (NAP) transcription factor mutant (nap) exhibited lower transcript levels of known chlorophyll degradation genes, STAY-GREEN1 (SGR1), NON-YELLOW COLORING1 (NYC1), PHEOPHYTINASE (PPH), and PHEIDE a OXYGENASE (PaO), and higher chlorophyll retention than the wild type during dark-induced senescence. Transcriptome coexpression analysis revealed that abscisic acid (ABA) metabolism/signaling genes were disproportionately represented among those positively correlated with NAP expression. ABA levels were abnormally low in nap leaves during extended darkness. The ABA biosynthetic genes 9-CIS-EPOXYCAROTENOID DIOXYGENASE2, ABA DEFICIENT3, and ABSCISIC ALDEHYDE OXIDASE3 (AAO3) exhibited abnormally low transcript levels in dark-treated nap leaves. NAP transactivated the promoter of AAO3 in mesophyll cell protoplasts, and electrophoretic mobility shift assays showed that NAP can bind directly to a segment (-196 to -162 relative to the ATG start codon) of the AAO3 promoter. Exogenous application of ABA increased the transcript levels of SGR1, NYC1, PPH, and PaO and suppressed the stay-green phenotype of nap leaves during extended darkness. Overexpression of AAO3 in nap leaves also suppressed the stay-green phenotype under extended darkness. Collectively, the results show that NAP promotes chlorophyll degradation by enhancing transcription of AAO3, which leads to increased levels of the senescence-inducing hormone ABA.

  6. Authentic Assessment in the First Steiner Academy

    ERIC Educational Resources Information Center

    Burnett, John

    2009-01-01

    In August 2008, the then Schools Minister, Andrew Adonis, gave the go-ahead for the privately funded Hereford Waldorf School to reopen as a tax-payer-funded Academy, sponsored by the Steiner Waldorf Schools Fellowship of Great Britain. Accordingly, the Steiner Academy Hereford opened in September 2008. In common with the 132 other Academies opened…

  7. Gifted and Talented Youth: The National Academy.

    ERIC Educational Resources Information Center

    Gifted Education International, 2003

    2003-01-01

    This article discusses England's development of an Academy for Gifted and Talented Youth. The academy will develop, implement, and support educational opportunities for gifted and talented children up to the age of 19, as well as provide support for parents and educators. The academy's talent search, outreach program, summer schools are described.…

  8. New Petrochemical Science and Technology Academy

    ERIC Educational Resources Information Center

    Majka, Connie

    2005-01-01

    In 1969, the first academy opened at Thomas Edison High School in Philadelphia. Called the Academy of Applied Electrical Science, the program was set up as an independent not-for-profit corporation with its own board of directors. Over the years, three more career areas--business, health and automotive--opened academies as independent entities. …

  9. Action Research at St Mark's Academy 2013

    ERIC Educational Resources Information Center

    Elwick, Alex, Ed.; Riggall, Anna, Ed.

    2013-01-01

    St Mark's Church of England Academy is an 11-18 academy situated in Mitcham, South London. It offers a commitment to high achievement within a community of care, underpinned by the Christian values of hope, love and trust. The academy encourages the development of the moral and spiritual well-being of students, alongside their academic success.…

  10. 32 CFR 575.1 - Military Academy.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY PERSONNEL ADMISSION TO THE UNITED STATES MILITARY ACADEMY § 575.1 Military Academy. (a) Organization and administration. (1) The United States.... (b) Mission. The mission of the United States Military Academy is to educate, train, and motivate...

  11. NapGH components of the periplasmic nitrate reductase of Escherichia coli K-12: location, topology and physiological roles in quinol oxidation and redox balancing.

    PubMed Central

    Brondijk, T Harma C; Nilavongse, Arjaree; Filenko, Nina; Richardson, David J; Cole, Jeffrey A

    2004-01-01

    Nap (periplasmic nitrate reductase) operons of many bacteria include four common, essential components, napD, napA, napB and napC (or a homologue of napC ). In Escherichia coli there are three additional genes, napF, napG and napH, none of which are essential for Nap activity. We now show that deletion of either napG or napH almost abolished Nap-dependent nitrate reduction by strains defective in naphthoquinone synthesis. The residual rate of nitrate reduction (approx. 1% of that of napG+ H+ strains) is sufficient to replace fumarate reduction in a redox-balancing role during growth by glucose fermentation. Western blotting combined with beta-galactosidase and alkaline phosphatase fusion experiments established that NapH is an integral membrane protein with four transmembrane helices. Both the N- and C-termini as well as the two non-haem iron-sulphur centres are located in the cytoplasm. An N-terminal twin arginine motif was shown to be essential for NapG function, consistent with the expectation that NapG is secreted into the periplasm by the twin arginine translocation pathway. A bacterial two-hybrid system was used to show that NapH interacts, presumably on the cytoplasmic side of, or within, the membrane, with NapC. As expected for a periplasmic protein, no NapG interactions with NapC or NapH were detected in the cytoplasm. An in vitro quinol dehydrogenase assay was developed to show that both NapG and NapH are essential for rapid electron transfer from menadiol to the terminal NapAB complex. These new in vivo and in vitro results establish that NapG and NapH form a quinol dehydrogenase that couples electron transfer from the high midpoint redox potential ubiquinone-ubiquinol couple via NapC and NapB to NapA. PMID:14674886

  12. Sleep Physiology in Toddlers: Effects of Missing a Nap on Subsequent Night Sleep.

    PubMed

    Lassonde, Jonathan M; Rusterholz, Thomas; Kurth, Salome; Schumacher, Allyson M; Achermann, Peter; LeBourgeois, Monique K

    2016-10-01

    The shift from a biphasic to a monophasic sleep schedule is a fundamental milestone in early childhood. This transition, however, may result in periods of acute sleep loss as children may nap on some but not all days. Although data indicating the behavioral consequences of nap deprivation in young children are accumulating, little is known about changes to sleep neurophysiology following daytime sleep loss. This study addresses this gap in knowledge by examining the effects of acute nap deprivation on subsequent nighttime sleep electroencephalographic (EEG) parameters in toddlers. Healthy children (n=25; 11 males; ages 30-36 months) followed a strict sleep schedule for ≥5 days before sleep EEG recordings performed on 2 non-consecutive days: one after 13 h of prior wakefulness and another at the same clock time but preceded by a daytime nap. Total slow-wave energy (SWE) was computed as cumulative slow-wave activity (SWA; EEG power in 0.75-4.5 Hz range) over time. Nap and subsequent night SWE were added and compared to SWE of the night after a missed nap. During the night following a missed nap, children fell asleep faster (11.9 ± 8.7 versus 37.3 ± 22.1 min; d=1.6, p=0.01), slept longer (10.1 ± 0.7 versus 9.6 ± 0.6 h; d=0.7, p<0.01) and exhibited greater SWA (133.3 ± 37.5 versus 93.0 ± 4.7 %; d=0.9, p<0.01) compared to a night after a daytime nap. SWE for combined nap and subsequent night sleep did not significantly differ from the night following nap deprivation (12141.1 ± 3872.9 versus 11588 ± 3270.8 µV(2*)h; d=0.6, p=0.12). However, compared to a night following a missed nap, children experienced greater time in bed (13.0±0.8 versus 10.9±0.5; d=3.1, p<0.01) and total sleep time (11.2±0.8 versus 10.1±0.7; d=1.4, p<0.01). Shorter sleep latency, longer sleep duration, and increased SWA in the night following a missed nap indicate that toddlers experience a physiologically meaningful homeostatic challenge after prolonged wakefulness. Whether toddlers

  13. Sleep Physiology in Toddlers: Effects of Missing a Nap on Subsequent Night Sleep

    PubMed Central

    Lassonde, Jonathan M.; Rusterholz, Thomas; Kurth, Salome; Schumacher, Allyson M.; Achermann, Peter; LeBourgeois, Monique K.

    2016-01-01

    The shift from a biphasic to a monophasic sleep schedule is a fundamental milestone in early childhood. This transition, however, may result in periods of acute sleep loss as children may nap on some but not all days. Although data indicating the behavioral consequences of nap deprivation in young children are accumulating, little is known about changes to sleep neurophysiology following daytime sleep loss. This study addresses this gap in knowledge by examining the effects of acute nap deprivation on subsequent nighttime sleep electroencephalographic (EEG) parameters in toddlers. Healthy children (n=25; 11 males; ages 30–36 months) followed a strict sleep schedule for ≥5 days before sleep EEG recordings performed on 2 non-consecutive days: one after 13 h of prior wakefulness and another at the same clock time but preceded by a daytime nap. Total slow-wave energy (SWE) was computed as cumulative slow-wave activity (SWA; EEG power in 0.75–4.5 Hz range) over time. Nap and subsequent night SWE were added and compared to SWE of the night after a missed nap. During the night following a missed nap, children fell asleep faster (11.9 ± 8.7 versus 37.3 ± 22.1 min; d=1.6, p=0.01), slept longer (10.1 ± 0.7 versus 9.6 ± 0.6 h; d=0.7, p<0.01) and exhibited greater SWA (133.3 ± 37.5 versus 93.0 ± 4.7 %; d=0.9, p<0.01) compared to a night after a daytime nap. SWE for combined nap and subsequent night sleep did not significantly differ from the night following nap deprivation (12141.1 ± 3872.9 versus 11588 ± 3270.8 µV2*h; d=0.6, p=0.12). However, compared to a night following a missed nap, children experienced greater time in bed (13.0±0.8 versus 10.9±0.5; d=3.1, p<0.01) and total sleep time (11.2±0.8 versus 10.1±0.7; d=1.4, p<0.01). Shorter sleep latency, longer sleep duration, and increased SWA in the night following a missed nap indicate that toddlers experience a physiologically meaningful homeostatic challenge after prolonged wakefulness. Whether toddlers

  14. A Guide to Press Relations.

    ERIC Educational Resources Information Center

    Fibre Box Association, Chicago, IL.

    Stressing that a positive press-relations program can be extremely helpful to businesses, this publication offers suggestions for establishing press contacts, preparing press releases, holding press conferences, illustrating a story, preparing materials for use in radio and television broadcasts, and developing policies to be followed in emergency…

  15. Status of automated nap-of-the-earth rotorcraft guidance

    NASA Technical Reports Server (NTRS)

    Coppenbarger, Richard A.; Cheng, Victor H. L.

    1991-01-01

    The status of automated nap-of-the-earth (NOE) flight guidance is discussed by examining two current research efforts that approach the problem of performing obstacle avoidance along a pre-planned course from different perspectives. The first, a real-time guidance system developed by Systems Technology Inc., focuses on the issue of pilot acceptable maneuvers, under the assumption of an ideal, but nonrealizable, obstacle detection system. The second, a system created at NASA Ames, concentrates on the utilization of realistic on-board sensor data for obstacle detection and avoidance-maneuver decisions. This paper examines the strengths and weaknesses of these two approaches, and how they might complement each other. The goal is an automatic NOE system that is both acceptable to pilots and capable of making intelligent use of realistic sensor information and path-selection logic.

  16. Technologies for automating rotorcraft nap-of-the-earth flight

    NASA Technical Reports Server (NTRS)

    Cheng, Victor H. L.; Sridhar, Banavar

    1992-01-01

    This paper discusses the technologies required for automating rotorcraft nap-of-the-earth flight, where the use of natural obstacles for masking from the enemy is intentional and the danger of undesirable obstacles such as enemy traps is real. Specifically, the automatic guidance structure is modeled by three decision-making levels: the far-field mission planning and the mid-field terrain-masking trajectory shaping are both driven by prestored terrain data, whereas the nearfield obstacle detection/avoidance is driven by real-time on-board sensor data. This paper summarizes the far-field and mid-field accomplishments, and reports on the status of the more-recent efforts in obstacle detection and avoidance development. Obstacle detection is based primarily on passive imaging sensors for the desirable properties of covertness and wide field of view, although active sensors are included in the structure to provide the much needed high resolution for thin-wire detection.

  17. Microtubule-stabilizing peptides and small molecules protecting axonal transport and brain function: focus on davunetide (NAP).

    PubMed

    Magen, Iddo; Gozes, Illana

    2013-12-01

    This review focuses on the therapeutic effects and mechanisms of action of NAP (davunetide), an eight amino acid snippet derived from activity-dependent neuroprotective protein (ADNP) which was discovered in our laboratory. We have recently described the effects of NAP in neurodegenerative disorders, and we now review the beneficial effects of NAP and other microtubule-stabilizing agents on impairments in axonal transport. Experiments in animal models of microtubule-deficiency including tauopathy (spanning from drosophila to mammals) showed protection of axonal transport by microtubule-stabilizers and NAP, which was coupled to motor and cognitive protection. Clinical trials with NAP (davunetide) are reviewed paving the path to future developments.

  18. Duration of sleep inertia after napping during simulated night work and in extended operations.

    PubMed

    Signal, Tracey Leigh; van den Berg, Margo J; Mulrine, Hannah M; Gander, Philippa H

    2012-07-01

    Due to the mixed findings of previous studies, it is still difficult to provide guidance on how to best manage sleep inertia after waking from naps in operational settings. One of the few factors that can be manipulated is the duration of the nap opportunity. The aim of the present study was to investigate the magnitude and time course of sleep inertia after waking from short (20-, 40- or 60-min) naps during simulated night work and extended operations. In addition, the effect of sleep stage on awakening and duration of slow wave sleep (SWS) on sleep inertia was assessed. Two within-subject protocols were conducted in a controlled laboratory setting. Twenty-four healthy young men (Protocol 1: n = 12, mean age = 25.1 yrs; Protocol 2: n = 12, mean age = 23.2 yrs) were provided with nap opportunities of 20-, 40-, and 60-min (and a control condition of no nap) ending at 02:00 h after ∼20 h of wakefulness (Protocol 1 [P1]: simulated night work) or ending at 12:00 h after ∼30 h of wakefulness (Protocol 2 [P2]: simulated extended operations). A 6-min test battery, including the Karolinska Sleepiness Scale (KSS) and the 4-min 2-Back Working Memory Task (WMT), was repeated every 15 min the first hour after waking. Nap sleep was recorded polysomnographically, and in all nap opportunities sleep onset latency was short and sleep efficiency high. Mixed-model analyses of variance (ANOVA) for repeated measures were calculated and included the factors time (time post-nap), nap opportunity (duration of nap provided), order (order in which the four protocols were completed), and the interaction of these terms. Results showed no test x nap opportunity effect (i.e., no effect of sleep inertia) on KSS. However, WMT performance was impaired (slower reaction time, fewer correct responses, and increased omissions) on the first test post-nap, primarily after a 40- or 60-min nap. In P2 only, performance improvement was evident 45 min post-awakening for naps of 40 min or more. In ANOVAs

  19. Recruitment of CG-NAP to the Golgi apparatus through interaction with dynein-dynactin complex.

    PubMed

    Kim, Hon-Song; Takahashi, Mikiko; Matsuo, Kazuhiko; Ono, Yoshitaka

    2007-03-01

    The structural organization and position of the Golgi apparatus are highly regulated by microtubule cytoskeleton and microtubule motor proteins. The mechanisms linking these proteins to the Golgi apparatus remain elusive. Here, we found that centrosome and Golgi-localized PKN associated protein (CG-NAP) was localized to the Golgi apparatus in a microtubule-dependent manner. Microtubule-binding experiments revealed that CG-NAP possessed two microtubule-binding domains. We also found that CG-NAP was well co-localized with cytoplasmic dynein subunits during recovery from the on-ice treatment of cells that induced dissociation of CG-NAP from the Golgi. Similar co-localization was observed during recovery from the acetate treatment, which has been reported to inhibit the dynein-mediated transport. CG-NAP was co-immunoprecipitated with a dynactin subunit p150(Glued). Expressing the p150(Glued)-binding region of CG-NAP fused with mitochondria-targeting sequence induced recruitment of mitochondria to the pericentriolar area, suggesting that this region interacts with functional cytoplasmic dynein in vivo. Moreover, over-expression of this region caused fragmentation of the Golgi similar to that of dynamitin. These results suggest that CG-NAP is recruited to the minus ends of microtubules by interacting with cytoplasmic dynein, thereby localizes to the Golgi apparatus in a microtubule-dependent manner and possibly involved in the formation of the Golgi near the centrosomes.

  20. Coordinated Action of Nap1 and RSC in Disassembly of Tandem Nucleosomes

    PubMed Central

    Prasad, Rashmi; D'Arcy, Sheena; Hada, Arjan; Luger, Karolin

    2016-01-01

    The SWI/SNF and RSC family of ATP-dependent chromatin remodelers disassembles nucleosomes by moving nucleosomes into the vicinity of adjoining nucleosomes. We found that the histone chaperone Nap1 efficiently promotes disassembly of adjacent nucleosomes with which RSC collides and not the disassembly of nucleosomes mobilized by RSC. Nap1 is specific to RSC, as it does not target SWI/SNF, its paralog in Saccharomyces cerevisiae. Extensive mutational analysis of Nap1 has revealed that Nap1 affinity for histones H2A-H2B and H3-H4 and its ability to displace histones from DNA are required for Nap1 to enhance RSC-mediated disassembly. Other histone chaperones, such as Vps75, that also bind histones are not able to enhance RSC-mediated disassembly. Our study suggests a mechanism by which Nap1 is recruited to actively transcribed regions and assists in the passage of the transcription complex through chromatin, and it provides a novel mechanism for the coordinated action of RSC and Nap1. PMID:27273866

  1. Anti-Inflammatory Properties of NAP in Acute Toxoplasma Gondii-Induced Ileitis in Mice.

    PubMed

    Heimesaat, Markus M; Fischer, André; Kühl, Anja A; Göbel, Ulf B; Gozes, Illana; Bereswill, Stefan

    2015-09-01

    The octapeptide NAP has been shown to exert neuroprotective properties. Here, we investigated potential anti-inflammatory effects of NAP in an acute ileitis model. To address this, C57BL/6j mice were perorally infected with Toxoplasma gondii (day 0). Within 1 week postinfection (p.i.), placebo (PLC)-treated mice developed acute ileitis due to Th1-type immune responses. Mice that were subjected to intraperitoneal NAP treatment from day 1 until day 6 p.i., however, developed less distinct macroscopic and microscopic disease as indicated by less body weight loss, less distinct histopathological ileal changes, and lower ileal apoptotic, but higher proliferating cell numbers, less abundance of neutrophils, macrophages, monocytes, and T lymphocytes, but higher numbers of regulatory T cells in the ileal mucosa and lamina propria, and lower concentrations of pro-inflammatory mediators in the ilea as compared to PLC controls at day 7 p.i. Remarkably, NAP-mediated anti-inflammatory effects could also be observed in extra-intestinal compartments including liver and spleen. Strikingly, lower MCP-1, TNF, and IL-12p70 serum concentrations in NAP as compared to PLC-treated mice at day 7 p.i. indicate a pronounced systemic anti-inflammatory effect of NAP in acute ileitis. These findings provide first evidence for NAP as a potential novel treatment option in intestinal inflammation.

  2. Neuroprotective effects of NAP against excitotoxic brain damage in the newborn mice: implications for cerebral palsy.

    PubMed

    Sokolowska, P; Passemard, S; Mok, A; Schwendimann, L; Gozes, I; Gressens, P

    2011-01-26

    Activity-dependent neuroprotective protein (ADNP) was shown to be essential for embryogenesis and brain development while NAP, an active motif of ADNP, is neuroprotective in a broad range of neurodegenerative disorders. In the present study, we examined the protective potential of ADNP/NAP in a mouse model of excitotoxic brain lesion mimicking brain damage associated with cerebral palsy. We demonstrated that NAP had a potent neuroprotective effect against ibotenate-induced excitotoxic damage in the cortical plate and the white matter of P5 mice, and moderate against brain lesions of P0 mice. In contrast, endogenous ADNP appears not to be involved in the response to excitotoxic challenge in the studied model. Our findings further show that NAP reduced the number of apoptotic neurons through activation of PI-3K/Akt pathway in the cortical plate or both PI-3K/Akt and MAPK/MEK1 kinases in the white matter. In addition, NAP prevented ibotenate-induced loss of pre-oligodendrocytes without affecting the number of astrocytes or activated microglia around the site of injection. These findings indicate that protective actions of NAP are mediated by triggering transduction pathways that are crucial for neuronal and oligodendroglial survival, thus, NAP might be a promising therapeutic agent for treating developing brain damage.

  3. Davunetide (NAP) protects the retina against early diabetic injury by reducing apoptotic death.

    PubMed

    Scuderi, Soraya; D'Amico, Agata Grazia; Castorina, Alessandro; Federico, Concetta; Marrazzo, Giuseppina; Drago, Filippo; Bucolo, Claudio; D'Agata, Velia

    2014-11-01

    Davunetide (NAP) is an eight amino acid peptide that has been shown to provide potent neuroprotection. In the present study, we investigated the neuroprotective effect of NAP in diabetic retinopathy using an in vivo streptozotocin (STZ)-induced diabetic model. A single intraocular injection of NAP (100 μg/mL) or vehicle was administered 1 week after STZ injection. Three weeks after diabetes induction, we assessed the retinal expression and distribution of apoptosis markers, cleaved caspase-3, and Bcl2, by Western blot and immunofluorescent analysis. Furthermore, we evaluated the activation of mitogen-activated protein kinase/extracellular signal-regulated protein kinase (MAPK/ERK) and/or phosphatidylinositol-3 kinase/Akt pathways by measuring the protein levels of p-ERK and p-AKT with or without NAP treatment. Results demonstrated that NAP treatment reduced apoptotic event in diabetic retina, and it restored cleaved caspase-3 expression levels in the retina of STZ-injected rats as well as the decreased Bcl2. NAP treatment improved cellular survival through the activation of the MAPK/ERK pathway. Taken together, these findings suggested that NAP might be useful to treat retinal degenerative diseases.

  4. Interactive effects of visuomotor perturbation and an afternoon nap on performance and the flow experience.

    PubMed

    Kaida, Kosuke; Itaguchi, Yoshihiro; Iwaki, Sunao

    2017-01-01

    The present study was designed (1) to clarify the relationship between the flow experience and improvements in visuomotor skills, (2) to examine the effects of rotating the axis of a computer mouse on visuomotor skills, and (3) to investigate the effects of sleep for improving visuomotor skills. Participants (N = 18) responded to Perturbation and nap (PER+Nap), No-perturbation and nap (NoPER+Nap) and Perturbation and rest (PER+Rest) conditions. In the PER+Nap condition, participants conducted a visuomotor tracking task using a computer mouse, which was accompanied by perturbation caused by rotating the axis of their mouse. After the task, they took a 90 min nap. In NoPER+Nap condition, they conducted the same visuomotor task without any perturbation and took a nap. In the PER+Rest condition, participants conducted the task with the perturbation and took a 90 min break spent reading magazines instead of taking a nap. Results indicated (1) the flow experience did not occur when participants' skills and the degree of the visuomotor challenge were matching, (2) improvements of visuomotor skills occurred regardless of the perturbation, (3) improvements of visuomotor skills occurred unrelated to the flow experience, or to mood states, and (4) improvements of visuomotor performance occurred regardless of sleep. These findings suggest that improvements of visuomotor skills occur regardless of mood status and occur independently of perturbations by axis rotation. The study also suggests that the acquisition of skills is related to merely the time elapsed since learning, rather than to sleep.

  5. Development of nap neurophysiology: preliminary insights into sleep regulation in early childhood.

    PubMed

    Kurth, Salome; Lassonde, Jonathan M; Pierpoint, Lauren A; Rusterholz, Thomas; Jenni, Oskar G; McClain, Ian J; Achermann, Peter; LeBourgeois, Monique K

    2016-12-01

    Although all young children nap, the neurophysiological features and associated developmental trajectories of daytime sleep remain largely unknown. Longitudinal studies of napping physiology are fundamental to understanding sleep regulation during early childhood, a sensitive period in brain and behaviour development and a time when children transition from a biphasic to a monophasic sleep-wakefulness pattern. We investigated daytime sleep in eight healthy children with sleep electroencephalography (EEG) assessments at three longitudinal points: 2 years (2.5-3.0 years), 3 years (3.5-4.0 years) and 5 years (5.5-6.0 years). At each age, we measured nap EEG during three randomized conditions: after 4 h (morning nap), 7 h (afternoon nap) and 10 h (evening nap) duration of prior wakefulness. Developmental changes in sleep were most prevalent in the afternoon nap (e.g. decrease in sleep duration by 30 min from 2 to 3 years and by 20 min from 3 to 5 years). In contrast, nap sleep architecture (% of sleep stages) remained unchanged across age. Maturational changes in non-rapid eye movement sleep EEG power were pronounced in the slow wave activity (SWA, 0.75-4.5 Hz), theta (4.75-7.75 Hz) and sigma (10-15 Hz) frequency ranges. These findings indicate that the primary marker of sleep depth, SWA, is less apparent in daytime naps as children mature. Moreover, our fundamental data provide insight into associations between sleep regulation and functional modifications in the central nervous system during early childhood.

  6. Interactive effects of visuomotor perturbation and an afternoon nap on performance and the flow experience

    PubMed Central

    2017-01-01

    The present study was designed (1) to clarify the relationship between the flow experience and improvements in visuomotor skills, (2) to examine the effects of rotating the axis of a computer mouse on visuomotor skills, and (3) to investigate the effects of sleep for improving visuomotor skills. Participants (N = 18) responded to Perturbation and nap (PER+Nap), No-perturbation and nap (NoPER+Nap) and Perturbation and rest (PER+Rest) conditions. In the PER+Nap condition, participants conducted a visuomotor tracking task using a computer mouse, which was accompanied by perturbation caused by rotating the axis of their mouse. After the task, they took a 90 min nap. In NoPER+Nap condition, they conducted the same visuomotor task without any perturbation and took a nap. In the PER+Rest condition, participants conducted the task with the perturbation and took a 90 min break spent reading magazines instead of taking a nap. Results indicated (1) the flow experience did not occur when participants’ skills and the degree of the visuomotor challenge were matching, (2) improvements of visuomotor skills occurred regardless of the perturbation, (3) improvements of visuomotor skills occurred unrelated to the flow experience, or to mood states, and (4) improvements of visuomotor performance occurred regardless of sleep. These findings suggest that improvements of visuomotor skills occur regardless of mood status and occur independently of perturbations by axis rotation. The study also suggests that the acquisition of skills is related to merely the time elapsed since learning, rather than to sleep. PMID:28182742

  7. Air Force Academy Aeronautics Digest.

    DTIC Science & Technology

    1984-03-01

    map the external flow field on the upper surface of the wing and fuselage, *Major, USAF, Associate Professor of Aeronautics, DFAN 2 7...research effort at the USAF Academy to establish the capabilities and limitations of the seven-hole pressure probe in mapping unknown flow fields. The ... map their locations. III. Apparatus A. Wind Tunnel The Subsonic Wind Tunnel in the Aeronautics Laboratory of

  8. Resolution of two native monomeric 90kDa nitrate reductase active proteins from Shewanella gelidimarina and the sequence of two napA genes.

    PubMed

    Simpson, Philippa J L; McKinzie, Audra A; Codd, Rachel

    2010-07-16

    The reduction of nitrate to nitrite in the bacterial periplasm occurs in the 90kDa NapA subunit of the periplasmic nitrate reductase (NAP) system. Most Shewanella genomes contain two nap operons: napEDABC and napDAGHB, which is an unusual feature of this genus. Two native, monomeric, 90kDa nitrate reductase active proteins were resolved by hydrophobic interaction chromatography from aerobic cultures of Shewanella gelidimarina replete with reduced nitrogen compounds. The 90kDa protein obtained in higher yield was characterized as NapA by electronic absorption and electron paramagnetic resonance spectroscopies and was identified by LC/MS/MS and MALDI-TOF/TOF MS as NapA from the napEDABC-type operon. The other 90kDa protein, which was unstable and produced in low yields, was posited as NapA from the napDAGHB-type operon. Two napA genes have been sequenced from the napEDABC-type and napDAGHB-type operons of S. gelidimarina. Native NAP from S. putrefaciens was resolved as one NapA monomer and one NapAB heterodimer. Two amino acid substitutions in NapA correlated with the isolation of NAP as a NapA monomer or a NapAB heterodimer. The resolution of native, redox-active NapA isoforms in Shewanella provides new insight into the respiratory versatility of this genus, which has implications in bioremediation and the assembly of microbial fuel cells.

  9. Optically Aligned Drill Press

    NASA Technical Reports Server (NTRS)

    Adderholdt, Bruce M.

    1994-01-01

    Precise drill press equipped with rotary-indexing microscope. Microscope and drill exchange places when turret rotated. Microscope axis first aligned over future hole, then rotated out of way so drill axis assumes its precise position. New procedure takes less time to locate drilling positions and produces more accurate results. Apparatus adapted to such other machine tools as milling and measuring machines.

  10. America's Enduring Ethnic Press.

    ERIC Educational Resources Information Center

    Marzolf, Marion

    Studies of the history of newspapers in the United States have virtually ignored the ethnically oriented, foreign language press. This gap in journalistic investigation should be filled by considering the two conflicting roles which ethnic newspapers fill: assimilation of the ethnic group into the mainstream of American culture and maintenance and…

  11. Meet the Press

    ERIC Educational Resources Information Center

    Quick, Brian L.

    2004-01-01

    Objective: To field questions from a room of students simulating a press conference. Type of speech: Impromptu. Point value: 10 participation points. To receive all 10 points, students must (1) address three questions from the lectern and ask three questions from their seat (3 points), (2) respond thoroughly to each question by providing a…

  12. Defining the University Press

    ERIC Educational Resources Information Center

    Fletcher, Janet

    1978-01-01

    Reports discussion at the annual meeting of the Association of American University Presses (AAUP) on such topics as overproduction, government support, "on demand" publishing, profitability, library photocopying, and selection in library acquisitions, as they relate to the roles of librarians and publishers. (JPF)

  13. The Bradyrhizobium japonicum napEDABC genes encoding the periplasmic nitrate reductase are essential for nitrate respiration.

    PubMed

    Delgado, María J; Bonnard, Nathalie; Tresierra-Ayala, Alvaro; Bedmar, Eulogio J; Müller, Peter

    2003-12-01

    The napEDABC gene cluster that encodes the periplasmic nitrate reductase from Bradyrhizobium japonicum USDA110 has been isolated and characterized. napA encodes the catalytic subunit, and the napB and napC gene products are predicted to be a soluble dihaem c and a membrane-anchored tetrahaem c-type cytochrome, respectively. napE encodes a transmembrane protein of unknown function, and the napD gene product is a soluble protein which is assumed to play a role in the maturation of NapA. Western blots of the periplasmic fraction from wild-type cells grown anaerobically with nitrate revealed the presence of a protein band with a molecular size of about 90 kDa corresponding to NapA. A B. japonicum mutant carrying an insertion in the napA gene was unable to grow under nitrate-respiring conditions, lacked nitrate reductase activity, and did not show the 90 kDa protein band. Complementation of the mutant with a plasmid bearing the napEDABC genes restored both nitrate-dependent anaerobic growth of the cells and nitrate reductase activity. A membrane-bound and a periplasmic c-type cytochrome, with molecular masses of 25 kDa and 15 kDa, respectively, were not detected in the napA mutant strain incubated anaerobically with nitrate, which identifies those proteins as the NapC and the NapB components of the B. japonicum periplasmic nitrate reductase enzyme. These results suggest that the periplasmic nitrate reductase is the enzyme responsible for anaerobic growth of B. japonicum under nitrate-respiring conditions. The promoter region of the napEDABC genes has been characterized by primer extension. A major transcript initiates 66.5 bp downstream of the centre of a putative FNR-like binding site.

  14. Physical Fitness of Police Academy Cadets: Baseline Characteristics and Changes During a 16-Week Academy

    PubMed Central

    Sherman, Ross A.; Crawley, William R.; Cosio-Lima, Ludmila M.

    2016-01-01

    Abstract Crawley, AA, Sherman, RA, Crawley, WR, and Cosio-Lima, LM. Physical fitness of police academy cadets: baseline characteristics and changes during a 16-week academy. J Strength Cond Res 30(5): 1416–1424, 2016—Police academies traditionally emphasize the importance of being physically fit. The purpose of this research was to determine cadet baseline physical fitness characteristics and assess effectiveness of a 16-week training program. Sixty-eight cadets (61 men, 7 women) volunteered to have baseline physical fitness characteristics assessed, and 55 cadets (49 men, 6 women) completed further testing at weeks 8 and 16. The testing comprised hand grip (strength), arm crank (upper-body power), 30 seconds Wingate (lower body power), sum of skinfolds and percentage body fat (body composition), 40-yard dash (sprint speed), 1 repetition maximum bench press (strength), T-test (agility), and sit-and-reach (flexibility). In addition, cadets completed standardized state testing (push-ups, sit-ups, vertical jump, and half-mile shuttle run). The training program consisted of 1 hour sessions, 3 d·wk−1, including aerobic, plyometrics, body weight, and resistance exercise. Significant changes were found in agility (p < 0.01), upper-body and lower-body peak power (p ≤ 0.05), sit-ups (p < 0.01), push-ups (p ≤ 0.05) across the first 8 weeks, and in agility (p ≤ 0.05), lower-body peak power (p ≤ 0.05), sit-ups (p < 0.01), push-ups (p ≤ 0.05), half-mile shuttle run (p < 0.01) across the full 16 weeks. However, none of the variables showed significant change across the second half of the program (weeks 8–16). A number of individual parameters of physical fitness showed evidence of improvement in the first 8 weeks, whereas none of the variables showed significant improvement in the second 8 weeks. This suggests modifications could be made to increase overall effectiveness of cadet physical training specifically after the 8-week mark. PMID:26466133

  15. Identification of Peach NAP Transcription Factor Genes and Characterization of their Expression in Vegetative and Reproductive Organs during Development and Senescence.

    PubMed

    Li, Fang; Li, Jinjin; Qian, Ming; Han, Mingyu; Cao, Lijun; Liu, Hangkong; Zhang, Dong; Zhao, Caiping

    2016-01-01

    The NAP (NAC-like, activated by AP3/P1) transcription factor belongs to a subfamily of the NAC transcription factor family, and is believed to have an important role in regulating plant growth and development. However, there is very little information about this subfamily in Rosaceous plants. We identified seven NAP genes in the peach genome. PpNAP2 was categorized in the NAP I group, and contained a conserved transcription activation region. The other PpNAP genes belonged to the NAP II group. The expression patterns of the PpNAP genes differed in various organs and developmental stages. PpNAP1 and PpNAP2 were highly expressed in mature and senescing flowers, but not in leaves, fruits, and flower buds. PpNAP3 and PpNAP5 were only expressed in leaves. The PpNAP4 expression level was high in mature and senescing fruits, while PpNAP6 and PpNAP7 expression was up-regulated in mature and senescent leaves and flowers. During the fruit development period, the PpNAP4 and PpNAP6 expression levels rapidly increased during the S1 and S4 stages, which suggests these genes are involved in the first exponential growth phase and fruit ripening. During the fruit ripening and softening period, the PpNAP1, PpNAP4, and PpNAP6 expression levels were high during the early storage period, which was accompanied by a rapid increase in ethylene production. PpNAP1, PpNAP4, and PpNAP6 expression slowly increased during the middle or late storage periods, and peaked at the end of the storage period. Additionally, abscisic acid (ABA)-treated fruits were softer and produced more ethylene than the controls. Furthermore, the PpNAP1, PpNAP4, and PpNAP6 expression levels were higher in ABA-treated fruits. These results suggest that PpNAP1, PpNAP4, and PpNAP6 are responsive to ABA and may regulate peach fruit ripening.

  16. Spread of epidemic Clostridium difficile NAP1/027 in Latin America: case reports in Panama.

    PubMed

    López-Ureña, Diana; Quesada-Gómez, Carlos; Miranda, Erick; Fonseca, Mercedes; Rodríguez-Cavallini, Evelyn

    2014-02-01

    The rate and severity of Clostridium difficile infection (CDI) have been linked to the emergence and spread of the hypervirulent toxigenic strain NAP1/027. This strain has been responsible for large outbreaks in healthcare facilities in North America and Europe and most recently in Latin America. This is the first report of the NAP1 strain in Panama. It suggests that the spread of C. difficile NAP1 throughout Latin America could be a possibility as evidenced in the following case reports. Five isolates typed as NAP1 had tcdA, tcdB, binary toxin gene cdtB and tcdC deletion. All isolates were resistant to clindamycin, fluoroquinolones and rifampicin. Under this scenario, surveillance programmes for CDI should be implemented in public health facilities in Latin America and diagnosis of CDI should be considered, especially in patients with predisposing factors.

  17. [Insomnia symptoms, daytime naps and physical leisure activities in the elderly: FIBRA Study Campinas].

    PubMed

    Monteiro, Natália Tonon; Neri, Anita Liberalesso; Coelim, Maria Filomena

    2014-04-01

    The practice of physical activities contributes to reducing the risk of chronic diseases and improves sleep patterns in the elderly. This research aimed to investigate the association between insomnia symptoms and daytime nap and the participation in physical leisure activities in elderly community residents. Data from the Studies Network of the Fragility in Brazilian Elderly (Campinas site), were used. Information from 689 elderly was analyzed, regarding sociodemographic characterization, physical leisure activity, occurrence of daytime napping and its duration, symptoms of insomnia and use of sleep medication. A significant association was found between the practice of walking and the daytime nap of short duration. Studies indicate that a short nap can benefit the quality of sleep and health of the elderly. Therefore, promoting the practice of walking can be a nursing intervention that favors the sleep patterns of the elderly.

  18. [Effect of daytime nap on consolidation of declarative memory in humans].

    PubMed

    Ukraintseva, Iu V; Dorokhov, V B

    2011-01-01

    We studied effects of a daytime nap (1 hour) with including only NREM sleep on performance of declarative memory task (60 semantically unrelated word pairs) and general functional state. During training, procedure of learning of 30 word pairs was presented once, and that of the other 30 pairs was repeated twice. Strength of the task acquisition was tested. Subjects participated in two experiments: basic and control one. After learning participants either took a nap (basic experiment) or kept awake looking movies (control experiment). In 4.5 hours after the training session all the subjects were retested. As compared to the subjects who stayed awake during the training-retesting interval, subjects who had a NREM nap demonstrated enhanced performance. Concerning the strength of task acquisition, sleep-dependent performance was observed only for the word pairs learned once. Naps did not affect the functional state assessed by the reaction time dynamics and psychological testing.

  19. Comparing the effects of nocturnal sleep and daytime napping on declarative memory consolidation.

    PubMed

    Lo, June C; Dijk, Derk-Jan; Groeger, John A

    2014-01-01

    Nocturnal sleep and daytime napping facilitate memory consolidation for semantically related and unrelated word pairs. We contrasted forgetting of both kinds of materials across a 12-hour interval involving either nocturnal sleep or daytime wakefulness (experiment 1) and a 2-hour interval involving either daytime napping or wakefulness (experiment 2). Beneficial effects of post-learning nocturnal sleep and daytime napping were greater for unrelated word pairs (Cohen's d=0.71 and 0.68) than for related ones (Cohen's d=0.58 and 0.15). While the size of nocturnal sleep and daytime napping effects was similar for unrelated word pairs, for related pairs, the effect of nocturnal sleep was more prominent. Together, these findings suggest that sleep preferentially facilitates offline memory processing of materials that are more susceptible to forgetting.

  20. The Concept of Qailulah (Midday Napping) from Neuroscientific and Islamic Perspectives.

    PubMed

    Tumiran, Mohd Amzari; Rahman, Noor Naemah Abdul; Saat, Rohaida Mohd; Kabir, Nurul; Zulkifli, Mohd Yakub; Adli, Durriyyah Sharifah Hasan

    2015-08-13

    Napping/siesta during the day is a phenomenon, which is widely practised in the world. However, the timing, frequency, and duration may vary. The basis of napping is also diverse, but it is mainly done for improvement in alertness and general well-being. Neuroscience reveals that midday napping improves memory, enhances alertness, boosts wakefulness and performance, and recovers certain qualities of lost night sleep. Interestingly, Islam, the religion of the Muslims, advocates midday napping primarily because it was a practice preferred by Prophet Muhammad (pbuh). The objectives of this review were to investigate and compare identical key points on focused topic from both neuroscientific and Islamic perspectives and make recommendations for future researches.

  1. Measurement of the thermal noise of a proton beam in the NAP-M storage ring

    SciTech Connect

    Dement'ev, E.M.; Dikanskii, N.S.; Medvedko, A.S.; Parkhomchuk, V.V.; Pestrikov, D.V.

    1980-08-01

    Measurements of the spectra and power of the noise of uncooled and cooled proton beams in the NAP-M storage ring are reported. Features of the noise of the cooled beam due to particle interaction are analyzed.

  2. THE NATIONAL RESEARCH COUNCIL REPORT ON MONITORED NATURAL ATTENUATION

    EPA Science Inventory

    The National Research Council recently released a report titled Natural Attenuation for Groundwater Remediation, available from the National Academy Press(http://www.nap.edu>). The report made a number of observations and recommedations, including the following. -Natural attenu...

  3. Who Speaks for Our Profession? Military and Strategic Studies at the USAF Academy

    DTIC Science & Technology

    2011-01-01

    Lewis Mumford claimed, “the Army has usually been the refuge of third-rate minds.”6 Clearly, the Academy preferred Clausewitz to Mumford , but...Princeton University Press, 1976), 88. 6 Lewis Mumford , Technics and Civilization (New York: Harcourt, Brace, and World, Inc., 1934), 95. Mumford ...credibility of Military Studies. Mumford was winning. In the seventies, Military Studies organized within a new CW organiza- tion, the Deputy

  4. Sleep inertia associated with a 10-min nap before the commute home following a night shift: A laboratory simulation study.

    PubMed

    Hilditch, Cassie J; Dorrian, Jillian; Centofanti, Stephanie A; Van Dongen, Hans P; Banks, Siobhan

    2017-02-01

    Night shift workers are at risk of road accidents due to sleepiness on the commute home. A brief nap at the end of the night shift, before the commute, may serve as a sleepiness countermeasure. However, there is potential for sleep inertia, i.e. transient impairment immediately after awakening from the nap. We investigated whether sleep inertia diminishes the effectiveness of napping as a sleepiness countermeasure before a simulated commute after a simulated night shift. N=21 healthy subjects (aged 21-35 y; 12 females) participated in a 3-day laboratory study. After a baseline night, subjects were kept awake for 27h for a simulated night shift. They were randomised to either receive a 10-min nap ending at 04:00 plus a 10-min pre-drive nap ending at 07:10 (10-NAP) or total sleep deprivation (NO-NAP). A 40-min York highway driving task was performed at 07:15 to simulate the commute. A 3-min psychomotor vigilance test (PVT-B) and the Samn-Perelli Fatigue Scale (SP-Fatigue) were administered at 06:30 (pre-nap), 07:12 (post-nap), and 07:55 (post-drive). In the 10-NAP condition, total pre-drive nap sleep time was 9.1±1.2min (mean±SD), with 1.3±1.9min spent in slow wave sleep, as determined polysomnographically. There was no difference between conditions in PVT-B performance at 06:30 (before the nap). In the 10-NAP condition, PVT-B performance was worse after the nap (07:12) compared to before the nap (06:30); no change across time was found in the NO-NAP condition. There was no significant difference between conditions in PVT-B performance after the drive. SP-Fatigue and driving performance did not differ significantly between conditions. In conclusion, the pre-drive nap showed objective, but not subjective, evidence of sleep inertia immediately after awakening. The 10-min nap did not affect driving performance during the simulated commute home, and was not effective as a sleepiness countermeasure.

  5. A Daytime Nap Facilitates Generalization of Word Meanings in Young Toddlers

    PubMed Central

    Horváth, Klára; Liu, Siying; Plunkett, Kim

    2016-01-01

    Study Objectives: One of the key processes in language development is generalization—the selection and extension of relevant features and information to similar objects and concepts. Little is known about how sleep influences generalization, and studies on the topic are inconclusive. Our aim was to investigate how a nap affects generalization in 16-mo-olds. We hypothesized that a nap is necessary for successful generalization of word meanings. Methods: Twenty-eight 16-mo-old, typically developing toddlers were randomly assigned to nap and wake groups. We trained toddlers with two novel object-word pairs and tested their initial ability to generalize. Toddlers took part in an intermodal preferential looking task, in which they were shown different colored versions of the original objects and heard one of the trained labels. If toddlers understand the label, they are expected to increase their looking time to the target. Looking behavior was measured with an automated eye tracker. Afterward, the nap group went to sleep, while the wake group stayed awake for approximately 2 h. We then repeated the test of their performance on the generalization task. Results: A significant interaction of group and session was found in preferential looking. The performance of the nap group increased after the nap, whereas that of the wake group did not change. Conclusions: Our results suggest that napping improves generalization in toddlers. Citation: Horváth K, Liu S, Plunkett K. A daytime nap facilitates generalization of word meanings in young toddlers. SLEEP 2016;39(1):203–207. PMID:26237777

  6. Objective Measurement of Daytime Napping, Cognitive Dysfunction and Subjective Sleepiness in Parkinson’s Disease

    PubMed Central

    Bolitho, Samuel J.; Naismith, Sharon L.; Salahuddin, Pierre; Terpening, Zoe; Grunstein, Ron R.; Lewis, Simon J. G.

    2013-01-01

    Introduction Sleep-wake disturbances and concomitant cognitive dysfunction in Parkinson’s disease (PD) contribute significantly to morbidity in patients and their carers. Subjectively reported daytime sleep disturbance is observed in over half of all patients with PD and has been linked to executive cognitive dysfunction. The current study used daytime actigraphy, a novel objective measure of napping and related this to neuropsychological performance in a sample of PD patients and healthy, age and gender-matched controls. Furthermore this study aimed to identify patients with PD who may benefit from pharmacologic and behavioural intervention to improve these symptoms. Methods Eighty-five PD patients and 21 healthy, age-matched controls completed 14 days of wrist actigraphy within two weeks of neuropsychological testing. Objective napping measures were derived from actigraphy using a standardised protocol and subjective daytime sleepiness was recorded by the previously validated Epworth Sleepiness Scale. Results Patients with PD had a 225% increase in the mean nap time per day (minutes) as recorded by actigraphy compared to age matched controls (39.2 ± 35.2 vs. 11.5 ± 11.0 minutes respectively, p < 0.001). Significantly, differences in napping duration between patients, as recorded by actigraphy were not distinguished by their ratings on the subjective measurement of excessive daytime sleepiness. Finally, those patients with excessive daytime napping showed greater cognitive deficits in the domains of attention, semantic verbal fluency and processing speed. Conclusion This study confirms increased levels of napping in PD, a finding that is concordant with subjective reports. However, subjective self-report measures of excessive daytime sleepiness do not robustly identify excessive napping in PD. Fronto-subcortical cognitive dysfunction was observed in those patients who napped excessively. Furthermore, this study suggests that daytime actigraphy, a non

  7. Protein profiling reveals antioxidant and signaling activities of NAP (Davunetide) in rodent hippocampus exposed to hypobaric hypoxia.

    PubMed

    Sethy, Niroj Kumar; Sharma, Narendra Kumar; Das, Mainak; Bhargava, Kalpana

    2014-11-01

    NAP (davunetide) is a clinical octapeptide and reportedly possesses neuroprotective, neurotrophic and cognitive protective properties. The information for NAP-mediated neuroproteome changes and associated signaling pathways during hypoxia will help in drug development programmes across the world. In the present study, we have evaluated the antioxidant activities of NAP in rat hippocampus exposed to hypobaric hypoxia (25,000 ft, 282 mm Hg) for 3, 6 and 12 h respectively. Using 2D-gel electrophoresis (2D-GE) with matrix-assisted laser desorption ionization time of flight (MALDI-TOF/TOF) mass spectrometry, we have identified altered expression of 80 proteins in NAP-supplemented hippocampus after hypoxia. Pathway analysis revealed that NAP supplementation significantly regulated oxidative stress response, oxidoreductase activity and cellular response to stress pathways during hypoxia. Additionally, NAP supplementation also regulated energy production pathways along with AMP-activated protein kinase (AMPK) signaling and signaling by Rho family GTPases pathways. We observed higher expression of antioxidant Sod1, Eno1, Prdx2 and Prdx5 proteins that were subsequently validated by Western blotting. A higher level of Prdx2 was also observed by immunohistochemistry in NAP-supplemented hippocampus during hypoxia. In corroboration, we are able to detect significant lower level of protein carbonyls in NAP-supplemented hypoxic hippocampus suggesting amelioration of oxidant molecules by NAP supplementation. These results emphasize the antioxidant and signaling properties of NAP in rodent hippocampus during hypobaric hypoxia.

  8. Diminished nap effects on memory consolidation are seen under oral contraceptive use.

    PubMed

    Genzel, Lisa; Bäurle, Anna; Potyka, Alina; Wehrle, Renate; Adamczyk, Marek; Friess, Elisabeth; Steiger, Axel; Dresler, Martin

    2014-01-01

    Many young females take exogenous hormones as oral contraceptive (OC), a condition rarely controlled for in studies on sleep and memory consolidation even though sex hormones influence consolidation. This study investigated the effects of OCs on sleep-related consolidation of a motor and declarative task, utilizing a daytime nap protocol. Fifteen healthy, young females taking OCs came to the sleep lab for three different conditions: nap with previous learning, wake with previous learning and nap without learning. They underwent each condition twice, once during the "pill-active" weeks and once during the "pill-free" week, resulting in 6 visits. In all conditions, participants showed a significant off-line consolidation effect, independent of pill week or nap/wake condition. There were no significant differences in sleep stage duration, spindle activity or spectral EEG frequency bands between naps with or without the learning condition. The present data showed a significant off-line enhancement in memory irrespective of potential beneficial effects of a nap. In comparison to previous studies, this may suggest that the use of OCs may enhance off-line memory consolidation in motor and verbal tasks per se. These results stress the importance to control for the use of OCs in studies focusing on memory performance.

  9. Patenting nonassociated polymeric structures (NAPS): implications for structural genomic data release.

    PubMed

    Sung, Lawrence M

    2003-01-01

    The intellectual property laws that govern patent rights should provide a reasonable balance between the competing concerns of open access and exclusivity. Open access can facilitate knowledge dissemination and collaboration in furthering science. On the other hand, exclusivity can ensure interest and financial investment in scientific research and development. In recent days, the appropriate balance between open access and exclusivity has been a focus of public debate, particularly with regard to genomic inventions and their applications. In seeking to reconcile the timing of structural genomic data release with certain efforts to secure intellectual property rights, the International Structural Genomics Organisation joins others confronting this controversy. This paper seeks to inform the discussion with an overview of the U.S. standards for patenting nonassociated polymeric structures (NAPS), which include polynucleotides or polypeptides of unknown biological significance, and their corresponding structural data. In the United States, the present ability to obtain patent rights to these discoveries appears problematic given the requirement of specific, substantial and credible utility, among other things. Without demonstrable utility, NAPS and NAPS-related data likely will not be entitled to patent protection, whether the U.S. Patent & Trademark Office rejects NAPS claims as unpatentable in the first instance, or the U.S. federal courts invalidate NAPS claims in later patent litigation. As such, the improbability of obtaining enforceable patent rights to NAPS might undermine the rationale for delaying structural genomic data release to allow for the filing of patent applications in this regard.

  10. Inhibitory effect of NAP-22 on the phosphatase activity of synaptojanin-1.

    PubMed

    Takaichi, Rika; Odagaki, Sin-Ichi; Kumanogoh, Haruko; Nakamura, Shun; Morita, Mitsuhiro; Maekawa, Shohei

    2012-01-01

    Endocytosis of the synaptic vesicle is a complicated process, in which many proteins and lipids participate. Phosphatidylinositol 4,5-bisphosphate (PIP(2) ) plays important roles in the process, and the dynamic regulation of this lipid is one of the key events. Synaptojanin is a PIP(2) phosphatase, and dephosphorylation of PIP(2) of the clathrin coated-vesicle results in the uncoating of the vesicle. NAP-22 is one of the major proteins of the neuronal detergent-resistant membrane microdomain and localizes in both the presynaptic plasma membrane and the synaptic vesicle. To elucidate the role of NAP-22 in synaptic function, a screening of the NAP-22 binding proteins through pull-down assay was performed. In addition to CapZ protein, synaptojanin-1 was detected by LC-MS/MS, and Western blotting using antisynaptojanin-1 confirmed this result. The interaction seems to be important in the course of synaptic vesicle endocytosis, because NAP-22 inhibited the phosphatase activity of synaptojanin in a dose-dependent manner. The inhibitory region for 5-phosphatase and the binding region for PIP(2) overlapped in the amino acid sequence of NAP-22, so elucidation of the regulatory mechanism of the PIP(2) binding ability of NAP-22 could be important in understanding the membrane dynamics at the presynaptic region.

  11. Risperidone and NAP protect cognition and normalize gene expression in a schizophrenia mouse model.

    PubMed

    Vaisburd, Sinaya; Shemer, Zeev; Yeheskel, Adva; Giladi, Eliezer; Gozes, Illana

    2015-11-10

    Mutated disrupted in schizophrenia 1 (DISC1), a microtubule regulating protein, leads to schizophrenia and other psychiatric illnesses. It is hypothesized that microtubule stabilization may provide neuroprotection in schizophrenia. The NAP (NAPVSIPQ) sequence of activity-dependent neuroprotective protein (ADNP) contains the SxIP motif, microtubule end binding (EB) protein target, which is critical for microtubule dynamics leading to synaptic plasticity and neuroprotection. Bioinformatics prediction for FDA approved drugs mimicking SxIP-like motif which displace NAP-EB binding identified Risperidone. Risperidone or NAP effectively ameliorated object recognition deficits in the mutated DISC1 mouse model. NAP but not Risperidone, reduced anxiety in the mutated mice. Doxycycline, which blocked the expression of the mutated DISC1, did not reverse the phenotype. Transcripts of Forkhead-BOX P2 (Foxp2), a gene regulating DISC1 and associated with human ability to acquire a spoken language, were increased in the hippocampus of the DISC1 mutated mice and were significantly lowered after treatment with NAP, Risperidone, or the combination of both. Thus, the combination of NAP and standard of care Risperidone in humans may protect against language disturbances associated with negative and cognitive impairments in schizophrenia.

  12. Risperidone and NAP protect cognition and normalize gene expression in a schizophrenia mouse model

    PubMed Central

    Vaisburd, Sinaya; Shemer, Zeev; Yeheskel, Adva; Giladi, Eliezer; Gozes, Illana

    2015-01-01

    Mutated disrupted in schizophrenia 1 (DISC1), a microtubule regulating protein, leads to schizophrenia and other psychiatric illnesses. It is hypothesized that microtubule stabilization may provide neuroprotection in schizophrenia. The NAP (NAPVSIPQ) sequence of activity-dependent neuroprotective protein (ADNP) contains the SxIP motif, microtubule end binding (EB) protein target, which is critical for microtubule dynamics leading to synaptic plasticity and neuroprotection. Bioinformatics prediction for FDA approved drugs mimicking SxIP-like motif which displace NAP-EB binding identified Risperidone. Risperidone or NAP effectively ameliorated object recognition deficits in the mutated DISC1 mouse model. NAP but not Risperidone, reduced anxiety in the mutated mice. Doxycycline, which blocked the expression of the mutated DISC1, did not reverse the phenotype. Transcripts of Forkhead-BOX P2 (Foxp2), a gene regulating DISC1 and associated with human ability to acquire a spoken language, were increased in the hippocampus of the DISC1 mutated mice and were significantly lowered after treatment with NAP, Risperidone, or the combination of both. Thus, the combination of NAP and standard of care Risperidone in humans may protect against language disturbances associated with negative and cognitive impairments in schizophrenia. PMID:26553741

  13. 75 FR 52318 - Presidential Academies for American History and Civics Education; Congressional Academies for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-25

    ... History and Civics Education; Congressional Academies for Students of American History and Civics...), as they apply to projects funded under the Presidential Academies for American History and Civics... for Students of American History and Civics Education program. These regulations,...

  14. Guidance automation for nap-of-the-earth flight

    NASA Technical Reports Server (NTRS)

    Denton, Richard V.; Pekelsma, Nick; Hagen, Mine; Mcgee, Leonard

    1986-01-01

    Low-Altitude/Nap-of-the-Earth (NOE) rotorcraft flight in adverse weather has been identified as a major technology void by both military and civil agencies because of its direct relationship to the needs of the military's new scout-attack helicopter and to various civilian applications including Emergency Medical Services (EMS). As part of NASA's Aircraft Automation Program, Ames Research Center has identified as a major goal the automating and advancing of applicable terrain following/terrain avoidance/obstacle avoidance (TF/TA/OA) guidance technologies to a level where NOE rotorcraft flight can be demonstrated in an advanced simulator and, later, in fully automatic flight. Areas of emphasis to date for achieving automatic guidance include real-time sensor blending and the real-time computation of NOE guidance commands. The present paper reports on the guidance command generation. The applicability of TF/TA techniques to the NOE problem is described, and simulation results are presented that suggest that automatic NOE can indeed be accomplished.

  15. 32 CFR 575.1 - Military Academy.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... the administration and training of the Corps of Cadets and is also head of the Department of Tactics. (b) Mission. The mission of the United States Military Academy is to educate, train, and motivate the... military training. In accomplishing its mission, the Military Academy strives to develop in each cadet...

  16. The Career Academy Concept. OJJDP Fact Sheet.

    ERIC Educational Resources Information Center

    Coffee, Joseph N.; Pestridge, Scott

    Career academies are schools within schools that link students with peers, teachers, and community partners in a disciplined environment, thereby fostering academic success and mental and emotional health. The career academy concept includes the following three key elements: (1) small learning communities; (2) a college preparatory curriculum with…

  17. Naval Academy Continues Its Diversity Trend

    ERIC Educational Resources Information Center

    Cooper, Kenneth J.

    2009-01-01

    Sixty years after graduating its first Black midshipman, Wesley Brown, the U.S. Naval Academy has admitted its most diverse class, which boasts the largest numbers and percentages of African-Americans and Hispanics ever to enter Annapolis. The academy has touted the racial and ethnic composition of the class of 2013 as the result of aggressive…

  18. Academies: A Breakthrough or Yet More Spin?

    ERIC Educational Resources Information Center

    Sinnott, Steve

    2008-01-01

    In this article the General Secretary of the National Union of Teachers outlines the reasons why the Union opposes Academies, and gives an overview of the changes in the relationship between local authorities and Academies. The NUT recognises that the change of government presents an opportunity for a change of direction and welcomes reports that…

  19. How Effective Are Military Academy Admission Standards

    DTIC Science & Technology

    2016-07-22

    Academy (USMA), and the U.S. Naval Academy (USNA) provide undergraduate education and training for stu- dents who will become commissioned officers...Reserve Officers Training Corps (ROTC) program (in the case of promotion outcomes). Unlike with USAFA, researchers did not find that a different

  20. Relationship between napping during night shift work and household obligations of female nursing personnel.

    PubMed

    Silva-Costa, Aline; Fischer, Frida Marina; Griep, Rosane Harter; Rotenberg, Lúcia

    2013-01-01

    Night shift employment involves displacing sleep to the daytime. For female workers, the opportunity for daytime sleep is influenced by routine housework demands, which aggravates sleep deprivation. Allowing naps to be taken during the night shift of work is a frequent practice at some hospitals and can help reduce the effects of sleep deprivation. We hypothesize that an association between domestic work and the length of naps during night work exists for nursing professionals. To test this hypothesis, two cross-sectional studies were conducted in two different hospitals. In Study 1, female workers answered questionnaires regarding sleeping habits, professional work, and housework demands. In Study 2, data regarding napping during shifts was obtained by actigraphy, a noninvasive method of monitoring the human sleep-wake cycle. The demand for the performance of housework was measured by (i) domestic work hours (total time spent performing domestic work per week), and (ii) domestic workload, which considers the degree of sharing domestic tasks and the number of people living at home. The populations from the two studies were subdivided into groups, based on the duration of napping at work. Data on naps were analyzed according to domestic demands, using the Mann-Whitney and Chi-squared tests. Among the two study populations (Studies 1 and 2), those in Study 2 were older, had shorter professional weekly work hours, worked more night shifts, and dedicated more time to housework. significant associations were only found in Study 2, where greater time napping at work was associated with both greater time spent doing housework and greater domestic workload. The known benefits of napping during night shifts seem to be especially relevant for female workers who are more sleep-deprived from working more night shifts and who have higher demands for housework.

  1. Anthropometric and Physical Profiles of English Academy Rugby Union Players.

    PubMed

    Darrall-Jones, Joshua D; Jones, Ben; Till, Kevin

    2015-08-01

    The purpose of this study was to evaluate the anthropometric and physical characteristics of English regional academy rugby union players by age category (under 16s, under 18s and under 21s). Data were collected on 67 academy players at the beginning of the preseason period and comprised anthropometric (height, body mass, and sum of 8 skinfolds) and physical (5-, 10-, 20-, and 40-m sprint, acceleration, velocity, and momentum; agility 505; vertical jump; Yo-Yo intermittent recovery test level 1; 30-15 intermittent fitness test; absolute and relative 3 repetition maximum front squat, split squat, bench press, prone row, and chin; and isometric mid-thigh pull). One-way analysis of variance demonstrated significant increases across the 3 age categories (p ≤ 0.05) for height (e.g., 16s = 178.8 ± 7.1; 18s = 183.5 ± 7.2; 21s = 186.7 ± 6.61 cm), body mass (e.g., 16s = 79.4 ± 12.8; 18s = 88.3 ± 11.9; 21s = 98.3 ± 10.4 kg), countermovement jump height and peak power, sprint momentum, velocity, and acceleration; absolute, relative, and isometric (e.g., 16s = 2,157.9 ± 309.9; 18s = 2,561.3 ± 339.4; 21s = 3,104.5 ± 354.0 N) strength. Momentum, maximal speed, and the ability to maintain acceleration were all discriminating factors between age categories, suggesting that these variables may be more important to monitor rather than sprint times. These findings highlight that anthropometric and physical characteristics develop across age categories and provide comparative data for English Academy Rugby Union players.

  2. American Academy of Facial Plastic and Reconstructive Surgery

    MedlinePlus

    ... Contact Us Shopping Cart American Academy of Facial Plastic and Reconstructive Surgery Home Meetings & Courses Find a ... About Our Academy The American Academy of Facial Plastic and Reconstructive Surgery is the world's largest specialty ...

  3. The relationship between subjective and objective sleepiness and performance during a simulated night-shift with a nap countermeasure.

    PubMed

    Tremaine, Rebecca; Dorrian, Jill; Lack, Leon; Lovato, Nicole; Ferguson, Sally; Zhou, Xuan; Roach, Greg

    2010-12-01

    The aim of the present study was to investigate the relationship between perceived and actual sleepiness and performance during a simulated night-shift that included a 30-min night-nap as an on-duty sleepiness countermeasure. Twenty-four healthy young adults (nine males, fifteen females) participated in a repeated measures design comprising two experimental conditions: no night-nap and 30-min night-nap. Both groups were given a 2-h prophylactic afternoon sleep opportunity (1500-1700 h). Measures of subjective sleepiness (Stanford Sleepiness Scale, Karolinska Sleepiness Scale and Visual Analogue Scale), objective sleepiness (sleep latency tests), objective performance (Symbol-Digit Substitution Task) and reaction time (Psychomotor Vigilance Task) were taken before the night-nap (0230 h) and at several intervals post-nap. Time-series correlation analyses indicated that subjective sleepiness was less correlated with objective sleepiness and objective performance when participants were given a 30-min night-nap. However subjective sleepiness and reaction time performance was strongly correlated in both conditions, and there was no significant difference between the nap and no-nap conditions. Consistent with previous research, results of the present study indicate that subjective and objective indicators of sleepiness and performance may not always correspond, and this relationship may be reduced by the inclusion of a night-nap.

  4. The NAP motif of activity-dependent neuroprotective protein (ADNP) regulates dendritic spines through microtubule end binding proteins.

    PubMed

    Oz, S; Kapitansky, O; Ivashco-Pachima, Y; Malishkevich, A; Giladi, E; Skalka, N; Rosin-Arbesfeld, R; Mittelman, L; Segev, O; Hirsch, J A; Gozes, I

    2014-10-01

    The NAP motif of activity-dependent neuroprotective protein (ADNP) enhanced memory scores in patients suffering from mild cognitive impairment and protected activities of daily living in schizophrenia patients, while fortifying microtubule (MT)-dependent axonal transport, in mice and flies. The question is how does NAP fortify MTs? Our sequence analysis identified the MT end-binding protein (EB1)-interacting motif SxIP (SIP, Ser-Ile-Pro) in ADNP/NAP and showed specific SxIP binding sites in all members of the EB protein family (EB1-3). Others found that EB1 enhancement of neurite outgrowth is attenuated by EB2, while EB3 interacts with postsynaptic density protein 95 (PSD-95) to modulate dendritic plasticity. Here, NAP increased PSD-95 expression in dendritic spines, which was inhibited by EB3 silencing. EB1 or EB3, but not EB2 silencing inhibited NAP-mediated cell protection, which reflected NAP binding specificity. NAPVSKIPQ (SxIP=SKIP), but not NAPVAAAAQ mimicked NAP activity. ADNP, essential for neuronal differentiation and brain formation in mouse, a member of the SWI/SNF chromatin remodeling complex and a major protein mutated in autism and deregulated in schizophrenia in men, showed similar EB interactions, which were enhanced by NAP treatment. The newly identified shared MT target of NAP/ADNP is directly implicated in synaptic plasticity, explaining the breadth and efficiency of neuroprotective/neurotrophic capacities.

  5. The Arecibo Observatory Space Academy

    NASA Astrophysics Data System (ADS)

    Rodriguez-Ford, Linda A.; Zambrano-Marin, Luisa; Petty, Bryan M.; Sternke, Elizabeth; Ortiz, Andrew M.; Rivera-Valentin, Edgard G.

    2015-11-01

    The Arecibo Observatory Space Academy (AOSA) is a ten (10) week pre-college research program for students in grades 9-12. Our mission is to prepare students for academic and professional careers by allowing them to receive an independent and collaborative research experience on topics related to space and aide in their individual academic and social development. Our objectives are to (1) Supplement the student’s STEM education via inquiry-based learning and indirect teaching methods, (2) Immerse students in an ESL environment, further developing their verbal and written presentation skills, and (3) To foster in every student an interest in science by exploiting their natural curiosity and knowledge in order to further develop their critical thinking and investigation skills. AOSA provides students with the opportunity to share lectures with Arecibo Observatory staff, who have expertise in various STEM fields. Each Fall and Spring semester, selected high school students, or Cadets, from all over Puerto Rico participate in this Saturday academy where they receive experience designing, proposing, and carrying out research projects related to space exploration, focusing on four fields: Physics/Astronomy, Biology, Engineering, and Sociology. Cadets get the opportunity to explore their topic of choice while practicing many of the foundations of scientific research with the goal of designing a space settlement, which they present at the NSS-NASA Ames Space Settlement Design Contest. At the end of each semester students present their research to their peers, program mentors, and Arecibo Observatory staff. Funding for this program is provided by NASA SSERVI-LPI: Center for Lunar Science and Exploration with partial support from the Angel Ramos Visitor Center through UMET and management by USRA.

  6. D-SAL and NAP: Two Peptides Sharing a SIP Domain.

    PubMed

    Gozes, Illana; Sragovich, Shlomo; Schirer, Yulie; Idan-Feldman, Anat

    2016-06-01

    NAPVSIPQ (NAP) and all D-amino acid SALLRSIPA (D-SAL) are neuroprotective peptides derived from activity-dependent neuroprotective protein (ADNP) and activity-dependent neurotrophic factor (ADNF), respectively. Both proteins were shown to protect against cognitive impairment, using different animal models and to increase neuronal survival following exposure to neurotoxins. NAP was extensively tested and found to increase microtubule stability, protect axonal transport, and inhibit apoptosis. Here, we aimed to further evaluate and correlate effects at the behavioral level, in a rat model of diabetes. Diabetes is primarily a metabolic disorder which presents secondary neurological manifestations. Diabetes induces peripheral nervous system damage which is translated into impaired sensory perception and is termed diabetic neuropathy. Diabetes-related central nervous system damage causes cognitive decline. The behavioral study aimed to evaluate the effect of NAP and D-SAL on peripheral neuropathy and cognitive decline. Peripheral neuropathy was tested by measuring the response to a thermal stimulus, and cognitive ability was measured by a social memory test and a spatial memory test using long- and short-term dependent tasks and a reference memory task. Results indicated an immediate sensory neuropathy in the diabetic model, which was prevented by both peptides and a later neuropathic phase, prevented only by NAP treatment. Cognitive tests revealed impaired performance in both social and spatial memory tests in the diabetes model. Each of the peptides improved different aspects of cognitive behavior, with NAP being more potent than D-SAL. Mechanistically, both NAP and SAL contain a SIP (SxIP) domain that has been shown to interact with microtubule end-binding proteins (EBs). Specifically, we have previously shown a direct interaction of NAP with EB1 and EB3; we have further shown an interaction of the NAP-derived 4 amino acid SKIP peptide with EB3, stimulating axonal

  7. Motor Performance Is not Enhanced by Daytime Naps in Older Adults

    PubMed Central

    Backhaus, Winifried; Braass, Hanna; Renné, Thomas; Gerloff, Christian; Hummel, Friedhelm C.

    2016-01-01

    The impact of sleep on motor learning in the aging brain was investigated using an experimental diurnal nap setup. As the brain ages several components of learning as well as motor performance change. In addition, aging is also related to sleep architectural changes. This combination of slowed learning processes and impaired sleep behavior raises the question of whether sleep can enhance learning and specifically performance of procedural tasks in healthy, older adults. Previous research was able to show sleep-dependent consolidation overnight for numerous tasks in young adults. Some of these study findings can also be replicated for older adults. This study aims to clarify whether sleep-dependent consolidation can also be found during shorter periods of diurnal sleep. The impact of midday naps on motor consolidation was analyzed by comparing procedural learning using a sequence and a motor adaptation task, in a crossover fashion in healthy, non-sleep deprived, older adults randomly subjected to wake (45 min), short nap (10–20 min sleep) or long nap (50–70 min sleep) conditions. Older adults exhibited learning gains, these were not found to be sleep-dependent in either task. The results suggest that daytime naps do not have an impact on performance and motor learning in an aging population. PMID:27303292

  8. The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway.

    PubMed

    Chen, Xu; Wang, Yaofeng; Lv, Bo; Li, Jie; Luo, Liqiong; Lu, Songchong; Zhang, Xuan; Ma, Hong; Ming, Feng

    2014-03-01

    Plants respond to environmental stresses by altering gene expression, and several genes have been found to mediate stress-induced expression, but many additional factors are yet to be identified. OsNAP is a member of the NAC transcription factor family; it is localized in the nucleus, and shows transcriptional activator activity in yeast. Analysis of the OsNAP transcript levels in rice showed that this gene was significantly induced by ABA and abiotic stresses, including high salinity, drought and low temperature. Rice plants overexpressing OsNAP did not show growth retardation, but showed a significantly reduced rate of water loss, enhanced tolerance to high salinity, drought and low temperature at the vegetative stage, and improved yield under drought stress at the flowering stage. Microarray analysis of transgenic plants overexpressing OsNAP revealed that many stress-related genes were up-regulated, including OsPP2C06/OsABI2, OsPP2C09, OsPP2C68 and OsSalT, and some genes coding for stress-related transcription factors (OsDREB1A, OsMYB2, OsAP37 and OsAP59). Our data suggest that OsNAP functions as a transcriptional activator that plays a role in mediating abiotic stress responses in rice.

  9. Nap and melatonin-induced changes in hippocampal activation and their role in verbal memory consolidation.

    PubMed

    Gorfine, Tali; Yeshurun, Yaara; Zisapel, Nava

    2007-11-01

    Overnight sleep contributes to memory consolidation; even a short nap improves memory performance. Such improvement has been linked to hippocampal activity during sleep. Melatonin has been shown to affect the human hippocampus and to induce 'sleep like' changes in brain activation. We therefore conducted and compared two functional magnetic resonance imaging studies: the first study assessed the effect of a 2-hr mid-day nap versus an equal amount of wakefulness on a verbal memory task (unrelated word pair association); the second assessed the effect of melatonin versus placebo (both conditions without nap) on a similar task. We report that following a nap relative to wakefulness, successful retrieval-related activation in the parahippocampus is decreased. A smaller decrease is seen in wakefulness with melatonin but not placebo. In parallel, an improvement in verbal memory recall was found after a nap compared with wakefulness but not with melatonin during wakefulness compared with placebo. Our findings demonstrate effects of melatonin that resemble those of sleep on verbal memory processing in the hippocampus thus suggesting that melatonin, like sleep, can initiate offline plastic changes underlying memory consolidation; they also suggest that concomitant rest without interferences is necessary for enhanced performance.

  10. Antidepressant effect of recombinant NT4-NAP/AAV on social isolated mice through intranasal route.

    PubMed

    Liu, Fei; Liu, You-Ping; Lei, Gang; Liu, Peng; Chu, Zheng; Gao, Cheng-Ge; Dang, Yong-Hui

    2017-02-07

    The purpose of the present study was to observe the depression-like behavior induced by social isolation; detect the antidepressant effect of a recombinant adeno-associated virus (AAV) expressing NAP on social isolation mice by intranasal delivery. After construction of NT4-NAP/AAV, expression of NAP was confirmed in vitro. 3-week-old C57/BL mice were bred individually in cages as social isolation-rearing. Six weeks later, the first subset of mice underwent behavioral tests and western blot; the second was for enzyme-linked immunosorbent assay. NT4-NAP/AAV was delivered quaque die by nasal administration for consecutive 10 days before behavioral test. Several depression-like behaviors were observed in social isolation mice, including decreased relative sucrose preference, longer immobility time in forced swimming test, lower plasma corticosterone and decreased brain-derived neurotrophic factor in hippocampus. Thus, social isolation procedure appears to be an animal model of depression with good face and construct validity. What's more, the antidepressant effect in social isolation-rearing mice was observed after intranasal administration of NT4-NAP/AAV, suggesting that this might be a promising therapeutic strategy for depressive disorder.

  11. Contact-dependent promotion of cell migration by the OL-protocadherin-Nap1 interaction.

    PubMed

    Nakao, Shinsuke; Platek, Anna; Hirano, Shinji; Takeichi, Masatoshi

    2008-07-28

    OL-protocadherin (OL-pc) is a transmembrane protein belonging to the cadherin superfamily, which has been shown to accumulate at cell-cell contacts via its homophilic interaction, but its molecular roles remain elusive. In this study, we show that OL-pc bound Nck-associated protein 1 (Nap1), a protein that regulates WAVE-mediated actin assembly. In astrocytoma U251 cells not expressing OL-pc, Nap1 was localized only along the lamellipodia. However, exogenous expression of OL-pc in these cells recruited Nap1 as well as WAVE1 to cell-cell contact sites. Although OL-pc expression had no effect on the motility of solitary U251 cells, it accelerated their movement when they were in contact with one another, causing concomitant reorganization of F-actin and N-cadherin at cell junctions. OL-pc mutants lacking the Nap1-binding site exhibited no such effect. N-cadherin knockdown mimicked OL-pc expression in enhancing cell movement. These results suggest that OL-pc remodels the motility and adhesion machinery at cell junctions by recruiting the Nap1-WAVE1 complex to these sites and, in turn, promotes the migration of cells.

  12. Anthropometric and physical characteristics of english academy rugby league players.

    PubMed

    Till, Kevin; Tester, Emma; Jones, Ben; Emmonds, Stacey; Fahey, Jack; Cooke, Carlton

    2014-02-01

    The purpose of the present study was to evaluate the anthropometric and physical characteristics of English academy rugby league players by annual-age category (under 16s-under 20s) and between backs and forwards. Data were collected on 133 academy players over a 6-year period (resulting in a total of 257 assessments). Player assessments comprised of anthropometric (height, body mass, sum of 4 skinfolds) and physical (vertical jump, 10- and 20-m sprint, estimated V[Combining Dot Above]O2max via the yo-yo intermittent recovery test level 1, absolute 1 repetition maximum [1RM], and relative squat, bench press, and prone row) measures. Univariate analysis of variance demonstrated significant (p ≤ 0.05) increases in height, body mass, vertical jump, absolute, and relative strength measures across the 5 annual-age categories (e.g., body mass: under 16s = 75.2 ± 11.1, under 20s = 88.9 ± 8.5 kg; vertical jump: under 16s = 45.7 ± 5.2, under 20s = 52.8 ± 5.4 cm; 1RM bench press: under 16s = 73.9 ± 13.2, under 20s = 114.3 ± 15.3 kg). Independent t-tests identified significant (p ≤ 0.05) differences between backs and forwards for anthropometric (e.g., under 16s body mass: backs = 68.4 ± 8.6, forwards = 80.9 ± 9.7 kg) and physical (e.g., under 19s 20-m sprint: backs = 3.04 ± 0.08, forwards = 3.14 ± 0.12s; under 18s relative squat: backs = 1.65 ± 0.18, forwards = 1.51 ± 0.17 kg·kg) characteristics that were dependent on the age category and measure assessed. Findings highlight that anthropometric and physical characteristics develop across annual-age categories and between backs and forwards in academy rugby league players. These findings provide comparative data for such populations and support the need to monitor player development in junior rugby league players.

  13. Functional characterization of human nucleosome assembly protein-2 (NAP1L4) suggests a role as a histone chaperone

    SciTech Connect

    Rodriguez, P.; Chu, Lee Lee; Kim, Jungho; Pelletier, J.

    1997-09-15

    Histones are thought to play a key role in regulating gene expression at the level of DNA packaging. Recent evidence suggests that transcriptional activation requires competition of transcription factors with histones for binding to regulatory regions and that there may be several mechanisms by which this is achieved. We have characterized a human nucleosome assembly protein, NAP-2, previously identified by positional cloning at 11p15.5, a region implicated in several disease processes including Wilms tumor (WT) etiology. The deduced amino acid sequence of NAP-2 indicates that it encodes a protein with a potential nuclear localization motif and two clusters of highly acidic residues. Functional analysis of recombinant NAP-2 protein purified from Escherichia coli demonstrates that this protein can interact with both core and linker histones. We demonstrate that recombinant NAP-2 can transfer histones onto naked DNA templates. Deletion mutagenesis of NAP-2 demonstrates that both NH3- and COOH-terminal domains are required for histone transfer activity. Subcellular localization studies of NAP-2 indicate that it can shuttle between the cytoplasm and the nucleus, suggesting a role as a histone chaperone. Given the potential role of the human NAP-2 gene (HGMW-approved symbol NAP1L4) in WT etiology, we have elucidated the exon/intron structure of this gene and have analyzed the mutational status of NAP-2 in sporadic WTs. Our results, coupled with tumor suppression assays in G401 WT cells, do not support a role for NAP-2 in the etiology of WT. A putative role for NAP-2 in regulating cellular differentiation is discussed. 59 refs., 7 figs., 1 tab.

  14. Slow wave sleep during a daytime nap is necessary for protection from subsequent interference and long-term retention.

    PubMed

    Alger, Sara E; Lau, Hiuyan; Fishbein, William

    2012-09-01

    While it is now generally accepted that sleep facilitates the processing of newly acquired declarative information, questions still remain as to the type and length of sleep necessary to best benefit declarative memories. A better understanding could lend support in one direction or another as to the much-debated role of sleep, be it passive, permissive, or active, in memory processing. The present study employed a napping paradigm and compared performance on a bimodal paired-associates task of those who obtained a 10-min nap, containing only Stages 1 and 2 sleep, to those whose nap contained slow-wave sleep (SWS) and rapid eye movement (REM) sleep (60-min nap), as well as to subjects who remained awake. Measurements were obtained for baseline performance at training, after a sleep/no sleep interval for short-term retention, after a subsequent stimulus-related interference task, and again after a weeklong retention period. While all groups learned the information similarly, both nap groups performed better than the Wake group when examining short-term retention, approximately 1.5h after training (10-min p=.052, 60-min p=.002). However, performance benefits seen in the 10-min nap group proved to be temporary. Performance after a stimulus-related interference task revealed significantly better memory retention in the 60-min nap group, with interference disrupting the memory trace far less than both the Wake and 10-min nap groups (p<.001, p=.006, respectively). After a weeklong retention period, sleep's benefit to memory persisted in the 60-min nap group, with performance significantly greater than both the Wake and 10-min nap groups (p<.001, p=.004, respectively). It is our conclusion that SWS, obtained only by those in the 60-min nap group, served to actively facilitate the consolidation of learned bimodal paired-associates, supported by theories such as the Standard Theory of Consolidation as well as the Synaptic Homeostasis Hypothesis.

  15. PRESS SHOP. SEVEN BLISS PRESSES STAMP OUT A VARIETY OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    PRESS SHOP. SEVEN BLISS PRESSES STAMP OUT A VARIETY OF CARTRIDGE AND SHELL CASINGS. THIS DEPARTMENT WAS TRANSFORMED FROM A MONEY-LOSING OPERATION TO A PROFIT CENTER UNDER THE FIRST WORKER-MANAGED QUALITY CIRCLE IN THE PLANT. - American Brass Foundry, 70 Sayre Street, Buffalo, Erie County, NY

  16. Stop and revive? The effectiveness of nap and active rest breaks for reducing driver sleepiness.

    PubMed

    Watling, Christopher N; Smith, Simon S; Horswill, Mark S

    2014-11-01

    The purpose of this study was to compare the effects of two commonly utilized sleepiness countermeasures: a nap break and an active rest break. The effects of the countermeasures were evaluated by physiological (EEG), subjective, and driving performance measures. Participants completed 2 h of simulated driving, followed by a 15-min nap break or a 15-min active rest break, then completed the final hour of simulated driving. The nap break reduced EEG and subjective sleepiness. The active rest break did not reduce EEG sleepiness, with sleepiness levels eventually increasing, and resulted in an immediate reduction of subjective sleepiness. No difference was found between the two breaks for the driving performance measure. The immediate reduction of subjective sleepiness after the active rest break could leave drivers with erroneous perceptions of their sleepiness, particularly with increases of physiological sleepiness after the break.

  17. Rapid-Eye-Movement-Sleep (REM) Associated Enhancement of Working Memory Performance after a Daytime Nap.

    PubMed

    Lau, Esther Yuet Ying; Wong, Mark Lawrence; Lau, Kristy Nga Ting; Hui, Florence Wai Ying; Tseng, Chia-huei

    2015-01-01

    The main objective was to study the impact of a daytime sleep opportunity on working memory and the mechanism behind such impact. This study adopted an experimental design in a sleep research laboratory. Eighty healthy college students (Age:17-23, 36 males) were randomized to either have a polysomnography-monitored daytime sleep opportunity (Nap-group, n=40) or stay awake (Wake-group, n=40) between the two assessment sessions. All participants completed a sleep diary and wore an actigraph-watch for 5 days before and one day after the assessment sessions. They completed the state-measurement of sleepiness and affect, in addition to a psychomotor vigilance test and a working memory task before and after the nap/wake sessions. The two groups did not differ in their sleep characteristics prior to and after the lab visit. The Nap-group had higher accuracy on the working memory task, fewer lapses on the psychomotor vigilance test and lower state-sleepiness than the Wake-group. Within the Nap-group, working memory accuracy was positively correlated with duration of rapid eye movement sleep (REM) and total sleep time during the nap. Our findings suggested that "sleep gain" during a daytime sleep opportunity had significant positive impact on working memory performance, without affecting subsequent nighttime sleep in young adult, and such impact was associated with the duration of REM. While REM abnormality has long been noted in pathological conditions (e.g. depression), which are also presented with cognitive dysfunctions (e.g. working memory deficits), this was the first evidence showing working memory enhancement associated with REM in daytime napping in college students, who likely had habitual short sleep duration but were otherwise generally healthy.

  18. Fatigue on the flight deck: the consequences of sleep loss and the benefits of napping.

    PubMed

    Hartzler, Beth M

    2014-01-01

    The detrimental effects of fatigue in aviation are well established, as evidenced by both the number of fatigue-related mishaps and numerous studies which have found that most pilots experience a deterioration in cognitive performance as well as increased stress during the course of a flight. Further, due to the nature of the average pilot's work schedule, with frequent changes in duty schedule, early morning starts, and extended duty periods, fatigue may be impossible to avoid. Thus, it is critical that fatigue countermeasures be available which can help to combat the often overwhelming effects of sleep loss or sleep disruption. While stimulants such as caffeine are typically effective at maintaining alertness and performance, such countermeasures do nothing to address the actual source of fatigue - insufficient sleep. Consequently, strategic naps are considered an efficacious means of maintaining performance while also reducing the individual's sleep debt. These types of naps have been advocated for pilots in particular, as opportunities to sleep either in the designated rest facilities or on the flight deck may be beneficial in reducing both the performance and alertness impairments associated with fatigue, as well as the subjective feelings of sleepiness. Evidence suggests that strategic naps can reduce subjective feelings of fatigue and improve performance and alertness. Despite some contraindications to implementing strategic naps while on duty, such as sleep inertia experienced upon awakening, both researchers and pilots agree that the benefits associated with these naps far outweigh the potential risks. This article is a literature review detailing both the health and safety concerns of fatigue among commercial pilots as well as benefits and risks associated with strategic napping to alleviate this fatigue.

  19. Toddler's self-regulation strategies in a challenge context are nap-dependent.

    PubMed

    Miller, Alison L; Seifer, Ronald; Crossin, Rebecca; Lebourgeois, Monique K

    2015-06-01

    Early childhood represents a time of developmental changes in both sleep and self-regulation, a construct reflecting the ability to control one's behaviour, attention and emotions when challenged. Links between sleep and self-regulation processes have been proposed, but experimental evidence with young children is lacking. In the current study, we tested the effects of acute sleep restriction (nap deprivation) on toddlers' self-regulation. Healthy children (n = 12; four males; aged 30-36 months (33.9 ± 1.7)) slept on a strict schedule (verified with actigraphy and sleep diaries) for 5 days before each of two afternoon assessments following a nap and a no-nap condition (~11-day protocol). Children were videotaped while attempting an unsolvable puzzle, and 10 mutually exclusive self-regulation strategies were later coded. On average, children lost ~90 min of sleep on the no-nap versus the nap day. Nap deprivation resulted in moderate-to-large effects on self-regulation strategies, with decreases in scepticism (d = 0.77; 7% change), negative self-appraisal (d = 0.92; 5% change) and increases in physical self-soothing (d = 0.68; 10% change), focus on the puzzle piece that would not fit (perseveration; d = 0.50; 9% change) and insistence on completing the unsolvable puzzle (d = 0.91; 10% change). Results suggest that sleep serves an important role in the way that toddlers respond to challenging events in their daily lives. After losing daytime sleep, toddlers were less able to engage effectively in a difficult task and reverted to less mature self-regulation strategies than when they were well rested. Over time, chronically missed sleep may impair young children's self-regulation abilities, resulting in risk for social-emotional, behavioural and school problems.

  20. Physical Fitness of Police Academy Cadets: Baseline Characteristics and Changes During a 16-Week Academy.

    PubMed

    Crawley, Amy A; Sherman, Ross A; Crawley, William R; Cosio-Lima, Ludmila M

    2016-05-01

    Police academies traditionally emphasize the importance of being physically fit. The purpose of this research was to determine cadet baseline physical fitness characteristics and assess effectiveness of a 16-week training program. Sixty-eight cadets (61 men, 7 women) volunteered to have baseline physical fitness characteristics assessed, and 55 cadets (49 men, 6 women) completed further testing at weeks 8 and 16. The testing comprised hand grip (strength), arm crank (upper-body power), 30 seconds Wingate (lower body power), sum of skinfolds and percentage body fat (body composition), 40-yard dash (sprint speed), 1 repetition maximum bench press (strength), T-test (agility), and sit-and-reach (flexibility). In addition, cadets completed standardized state testing (push-ups, sit-ups, vertical jump, and half-mile shuttle run). The training program consisted of 1 hour sessions, 3 d·wk, including aerobic, plyometrics, body weight, and resistance exercise. Significant changes were found in agility (p < 0.01), upper-body and lower-body peak power (p ≤ 0.05), sit-ups (p < 0.01), push-ups (p ≤ 0.05) across the first 8 weeks, and in agility (p ≤ 0.05), lower-body peak power (p ≤ 0.05), sit-ups (p < 0.01), push-ups (p ≤ 0.05), half-mile shuttle run (p < 0.01) across the full 16 weeks. However, none of the variables showed significant change across the second half of the program (weeks 8-16). A number of individual parameters of physical fitness showed evidence of improvement in the first 8 weeks, whereas none of the variables showed significant improvement in the second 8 weeks. This suggests modifications could be made to increase overall effectiveness of cadet physical training specifically after the 8-week mark.

  1. KSHV encoded LANA recruits Nucleosome Assembly Protein NAP1L1 for regulating viral DNA replication and transcription

    PubMed Central

    Gupta, Namrata; Thakker, Suhani; Verma, Subhash C.

    2016-01-01

    The establishment of latency is an essential for lifelong persistence and pathogenesis of Kaposi’s sarcoma-associated herpesvirus (KSHV). Latency-associated nuclear antigen (LANA) is the most abundantly expressed protein during latency and is important for viral genome replication and transcription. Replication-coupled nucleosome assembly is a major step in packaging the newly synthesized DNA into chromatin, but the mechanism of KSHV genome chromatinization post-replication is not understood. Here, we show that nucleosome assembly protein 1-like protein 1 (NAP1L1) associates with LANA. Our binding assays revealed an association of LANA with NAP1L1 in KSHV-infected cells, which binds through its amino terminal domain. Association of these proteins confirmed their localization in specific nuclear compartments of the infected cells. Chromatin immunoprecipitation assays from NAP1L1-depleted cells showed LANA-mediated recruitment of NAP1L1 at the terminal repeat (TR) region of the viral genome. Presence of NAP1L1 stimulated LANA-mediated DNA replication and persistence of a TR-containing plasmid. Depletion of NAP1L1 led to a reduced nucleosome positioning on the viral genome. Furthermore, depletion of NAP1L1 increased the transcription of viral lytic genes and overexpression decreased the promoter activities of LANA-regulated genes. These results confirmed that LANA recruitment of NAP1L1 helps in assembling nucleosome for the chromatinization of newly synthesized viral DNA. PMID:27599637

  2. NAP1 acts with Clb1 to perform mitotic functions and to suppress polar bud growth in budding yeast

    PubMed Central

    1995-01-01

    NAP1 is a 60-kD protein that interacts specifically with mitotic cyclins in budding yeast and frogs. We have examined the ability of the yeast mitotic cyclin Clb2 to function in cells that lack NAP1. Our results demonstrate that Clb2 is unable to carry out its full range of functions without NAP1, even though Clb2/p34CDC28-associated kinase activity rises to normal levels. In the absence of NAP1, Clb2 is unable to efficiently induce mitotic events, and cells undergo a prolonged delay at the short spindle stage with normal levels of Clb2/p34CDC28 kinase activity. NAP1 is also required for the ability of Clb2 to induce the switch from polar to isotropic bud growth. As a result, polar bud growth continues during mitosis, giving rise to highly elongated cells. Our experiments also suggest that NAP1 is required for the ability of the Clb2/p34CDC28 kinase complex to amplify its own production, and that NAP1 plays a role in regulation of microtubule dynamics during mitosis. Together, these results demonstrate that NAP1 is required for the normal function of the activated Clb2/p34CDC28 kinase complex, and provide a step towards understanding how cyclin- dependent kinase complexes induce specific events during the cell cycle. PMID:7622567

  3. A Brief Nap Is Beneficial for Human Route-Learning: The Role of Navigation Experience and EEG Spectral Power

    ERIC Educational Resources Information Center

    Wamsley, Erin J.; Tucker, Matthew A.; Payne, Jessica D.; Stickgold, Robert

    2010-01-01

    Here, we examined the effect of a daytime nap on changes in virtual maze performance across a single day. Participants either took a short nap or remained awake following training on a virtual maze task. Post-training sleep provided a clear performance benefit at later retest, but only for those participants with prior experience navigating in a…

  4. Afternoon Nap and Bright Light Exposure Improve Cognitive Flexibility Post Lunch

    PubMed Central

    Schmitz, Rémy; Peigneux, Philippe; Leproult, Rachel

    2015-01-01

    Beneficial effects of napping or bright light exposure on cognitive performance have been reported in participants exposed to sleep loss. Nonetheless, few studies investigated the effect of these potential countermeasures against the temporary drop in performance observed in mid-afternoon, and even less so on cognitive flexibility, a crucial component of executive functions. This study investigated the impact of either an afternoon nap or bright light exposure on post-prandial alterations in task switching performance in well-rested participants. Twenty-five healthy adults participated in two randomized experimental conditions, either wake versus nap (n=15), or bright light versus placebo (n=10). Participants were tested on a switching task three times (morning, post-lunch and late afternoon sessions). The interventions occurred prior to the post-lunch session. In the nap/wake condition, participants either stayed awake watching a 30-minute documentary or had the opportunity to take a nap for 30 minutes. In the bright light/placebo condition, participants watched a documentary under either bright blue light or dim orange light (placebo) for 30 minutes. The switch cost estimates cognitive flexibility and measures task-switching efficiency. Increased switch cost scores indicate higher difficulties to switch between tasks. In both control conditions (wake or placebo), accuracy switch-cost score increased post lunch. Both interventions (nap or bright light) elicited a decrease in accuracy switch-cost score post lunch, which was associated with diminished fatigue and decreased variability in vigilance. Additionally, there was a trend for a post-lunch benefit of bright light with a decreased latency switch-cost score. In the nap group, improvements in accuracy switch-cost score were associated with more NREM sleep stage N1. Thus, exposure to bright light during the post-lunch dip, a countermeasure easily applicable in daily life, results in similar beneficial effects as

  5. Afternoon nap and bright light exposure improve cognitive flexibility post lunch.

    PubMed

    Slama, Hichem; Deliens, Gaétane; Schmitz, Rémy; Peigneux, Philippe; Leproult, Rachel

    2015-01-01

    Beneficial effects of napping or bright light exposure on cognitive performance have been reported in participants exposed to sleep loss. Nonetheless, few studies investigated the effect of these potential countermeasures against the temporary drop in performance observed in mid-afternoon, and even less so on cognitive flexibility, a crucial component of executive functions. This study investigated the impact of either an afternoon nap or bright light exposure on post-prandial alterations in task switching performance in well-rested participants. Twenty-five healthy adults participated in two randomized experimental conditions, either wake versus nap (n=15), or bright light versus placebo (n=10). Participants were tested on a switching task three times (morning, post-lunch and late afternoon sessions). The interventions occurred prior to the post-lunch session. In the nap/wake condition, participants either stayed awake watching a 30-minute documentary or had the opportunity to take a nap for 30 minutes. In the bright light/placebo condition, participants watched a documentary under either bright blue light or dim orange light (placebo) for 30 minutes. The switch cost estimates cognitive flexibility and measures task-switching efficiency. Increased switch cost scores indicate higher difficulties to switch between tasks. In both control conditions (wake or placebo), accuracy switch-cost score increased post lunch. Both interventions (nap or bright light) elicited a decrease in accuracy switch-cost score post lunch, which was associated with diminished fatigue and decreased variability in vigilance. Additionally, there was a trend for a post-lunch benefit of bright light with a decreased latency switch-cost score. In the nap group, improvements in accuracy switch-cost score were associated with more NREM sleep stage N1. Thus, exposure to bright light during the post-lunch dip, a countermeasure easily applicable in daily life, results in similar beneficial effects as

  6. The CIA and the Press.

    ERIC Educational Resources Information Center

    Carvalho, Bernardo A.

    The involvement of the Central Intelligence Agency (CIA) with both United States and foreign news media has been recorded in numerous publications. This report reviews the important aspects of the CIA-press relationships as they have appeared in print and discusses the implications of these relationsihps for the credibility of the press. Media…

  7. Press Secretaries: A Brief Look.

    ERIC Educational Resources Information Center

    Rogers, Hines E., III

    The role of press secretary has attained a new significance during the past ten years because of presidential efforts to concentrate the focus of news on the White House. Originally intended to serve the public, the press secretary is now an important government official who not only conveys information but also explains and defends presidential…

  8. 78 FR 33836 - CPSC Safety Academy

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-05

    ... toy standard, navigating compliance issues, and the fast track recall program. The Safety Academy is...; (Panel 2) CPSC Processes continued, including Fast Track and Section 15; and (Panel 3) Flammable...

  9. Air Force Academy MS4 NPDES Permit

    EPA Pesticide Factsheets

    Under NPDES permit CO-R042007, the U.S. Air Force Academy is authorized to discharge from all municipal separate storm sewer system outfalls to the receiving waters specified in the permit in El Paso County, Colorado.

  10. Foundation of the American Academy of Ophthalmology

    MedlinePlus

    ... Plastic Surgery Center Laser Surgery Education Center Redmond Ethics Center Global Ophthalmology Guide Academy Publications EyeNet Ophthalmology Information for: International Ophthalmologists Media Medical Students Patients and Public Technicians and Nurses ...

  11. American Academy of Neurological and Orthopaedic Surgeons

    MedlinePlus

    ... Washington – June 16-17, 2017 at the Marriott Hotel (SEATAC). Our Mission Our Mission The Academy was ... event in Seattle, Washington at the Airport Marriott Hotel where a highly informative educational program has been ...

  12. Einstein Session of the Pontifical Academy.

    ERIC Educational Resources Information Center

    Science, 1980

    1980-01-01

    The texts of four speeches, given at the 1979 Einstein Session of the Pontifical Academy held in Rome, are presented. Each address relates to some aspect of the life and times of Albert Einstein. (SA)

  13. American Academy of Dental Sleep Medicine

    MedlinePlus

    ... Dentist Applications Now Accepted Join Us in Boston! Membership Join the American Academy of Dental Sleep Medicine ... and more. View Member Benefits... Join Now Renew Membership Diplomate Certification The American Board of Dental Sleep ...

  14. Functional roles of CymA and NapC in reduction of nitrate and nitrite by Shewanella putrefaciens W3-18-1

    SciTech Connect

    Beliav, Alex; Qiu, Dongru; Fredrickson, James K.; Wei, Hehong; Nealson, Kenneth H.; Xia, Ming; Zhou, Jizhong; Dai, Jingcheng; Shi, Liang; Tiedje, James M.; Romine, Margaret F.

    2016-06-01

    Shewanella putrefaciens W3-18-1 harbours two periplasmic nitrate reductase (Nap) gene clusters, NapC-associated nap-alpha (napEDABC) and CymA-dependent nap-beta (napDAGHB), for dissimilatory nitrate respiration. CymA is a member of the NapC/NirT quinol dehydrogenase family and acts as a hub to support different respiratory pathways, including those on iron [Fe(III)] and manganese [Mn(III, IV)] (hydr)oxide, nitrate, nitrite, fumarate and arsenate in Shewanella strains. However, in our analysis it was shown that another NapC/NirT family protein, NapC, was only involved in nitrate reduction, although both CymA and NapC can transfer quinol-derived electrons to a periplasmic terminal reductase or an electron acceptor. Furthermore, our results showed that NapC could only interact specifically with the Nap-alpha nitrate reductase while CymA could interact promiscuously with Nap-alpha, Nap-beta and the NrfA nitrite reductase for nitrate and nitrite reduction. To further explore the difference in specificity, site-directed mutagenesis on both CymA and NapC was conducted and the phenotypic changes in nitrate and nitrite reduction were tested. Our analyses demonstrated that the Lys-91 residue played a key role in nitrate reduction for quinol oxidation and the Asp-166 residue might influence the maturation of CymA. The Asp-97 residue might be one of the key factors that influence the interaction of CymA with the cytochromes NapB and NrfA.

  15. Nap it or leave it in the elderly: A nap after practice relaxes age-related limitations in procedural memory consolidation.

    PubMed

    Korman, M; Dagan, Y; Karni, A

    2015-10-08

    Using a training protocol that effectively induces procedural memory consolidation (PMC) in young adults, we show that older adults are good learners, robustly improving their motor performance during training. However, performance declined over the day, and overnight 'offline' consolidation phase performance gains were under-expressed. A post-training nap countered these deficits. PMC processes are preserved but under-engaged in the elderly; sleep can relax some of the age-related constraints on long-term plasticity.

  16. The Bulgarian Academy of Sciences in Transition from Learned Society to Totalitarian Academy (1944 1949)

    NASA Astrophysics Data System (ADS)

    Vavrek, Alexander

    The process of transforming the Bulgarian Academy of Sciences and Arts, founded in 1869 as a Bulgarian Learned Society, into a national research center began in 1940 and was significantly accelerated in 1944, immediately after the coup d''état of September 9, 1944, called during the last 50 years a ''socialist revolution''. Strong pressure was exerted on the Bulgarian ''bourgeois intelligentsia'' by the new Fatherland Front ruling circles controlled by the communists. Closing down of the old and appointing a new ''progressive'' Academy was also discussed. The urgent actions of the Executive Council of the Academy prevented these plans. A number of progressive-minded scholars and artists were elected to the Bulgarian Academy of Sciences and Arts in January 1945 and July 1946, and a plan for reorganizing the Academy was approved in November 1945. This opening stage of self-restructuring of the Academy was crossed out by the Law of the Bulgarian Academy of Sciences of 1947. By this law the Academy was transformed into a governmental organization, but some academic autonomy and respect for the academic traditions were preserved. Only two and a half years later, however, when the Bulgarian Communist Party had an absolute majority in the Parliament and the ''open building of socialism in the People''s Republic of Bulgaria'' had been already announced, a new, completely totalitarian, Law on the Bulgarian Academy of Sciences was passed.

  17. 32 CFR 575.6 - Catalogue, United States Military Academy.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 32 National Defense 3 2014-07-01 2014-07-01 false Catalogue, United States Military Academy. 575.6... ADMISSION TO THE UNITED STATES MILITARY ACADEMY § 575.6 Catalogue, United States Military Academy. The latest edition of the catalogue, United States Military Academy, contains additional...

  18. 32 CFR 575.6 - Catalogue, United States Military Academy.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 3 2011-07-01 2009-07-01 true Catalogue, United States Military Academy. 575.6... ADMISSION TO THE UNITED STATES MILITARY ACADEMY § 575.6 Catalogue, United States Military Academy. The latest edition of the catalogue, United States Military Academy, contains additional...

  19. 32 CFR 575.6 - Catalogue, United States Military Academy.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 32 National Defense 3 2012-07-01 2009-07-01 true Catalogue, United States Military Academy. 575.6... ADMISSION TO THE UNITED STATES MILITARY ACADEMY § 575.6 Catalogue, United States Military Academy. The latest edition of the catalogue, United States Military Academy, contains additional...

  20. 32 CFR 575.6 - Catalogue, United States Military Academy.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Catalogue, United States Military Academy. 575.6... ADMISSION TO THE UNITED STATES MILITARY ACADEMY § 575.6 Catalogue, United States Military Academy. The latest edition of the catalogue, United States Military Academy, contains additional...

  1. 32 CFR 575.6 - Catalogue, United States Military Academy.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 3 2013-07-01 2013-07-01 false Catalogue, United States Military Academy. 575.6... ADMISSION TO THE UNITED STATES MILITARY ACADEMY § 575.6 Catalogue, United States Military Academy. The latest edition of the catalogue, United States Military Academy, contains additional...

  2. Sex difference in the association between habitual daytime napping and prevalence of diabetes: a population-based study.

    PubMed

    Sun, Kan; Li, Feng; Qi, Yiqin; Lin, Diaozhu; Ren, Meng; Xu, Mingtong; Li, Fangping; Li, Yan; Yan, Li

    2016-05-01

    Our objective was to evaluate the associations between habitual daytime napping and diabetes and whether it varies by sex, menopause, and sleep quality. We conducted a population-based cross-sectional study in 8621 eligible individuals aged 40 years or older. Information on daytime napping hours, night-time sleep duration, history of menstruation, and sleep quality was self-reported. Diabetes was diagnosed according to the 1999 World Health Organization diagnostic criteria. The prevalence of diabetes was 19.4 % in men and 15.6 % in women. Increased daytime napping hours were positively associated with parameters of glycometabolism in women, such as fasting plasma glucose, oral glucose tolerance test (OGTT) 2-h plasma glucose, and Hemoglobin A1c (HbA1c, all P for trend <0.05). In women, the prevalence of diabetes in no-habitual daytime napping group, 0-1-h daytime napping group, and more than 1-h daytime napping group were 14.5, 15.6, and 20.8 %, respectively (P for trend = 0.0004). A similar trend was detected in postmenopausal women (P for trend = 0.002). In multivariate logistic regression analysis, compared with no-habitual daytime napping postmenopausal women, those with daytime napping more than 1 h had higher prevalent diabetes (odds ratios 1.36, 95 % confidence interval, 1.04-1.77). In subgroup analysis of postmenopausal women, associations of daytime napping levels and prevalent diabetes were detected in older, overweight participants with good sleep quality who have not retired from work. In conclusion, our study suggests that habitual daytime napping is associated with prevalence of diabetes in postmenopausal women.

  3. ADNP/NAP dramatically increase microtubule end-binding protein-Tau interaction: a novel avenue for protection against tauopathy.

    PubMed

    Ivashko-Pachima, Y; Sayas, C Laura; Malishkevich, A; Gozes, I

    2017-01-24

    Activity-dependent neuroprotective protein (ADNP), vital for brain formation and cognitive function, is mutated in autism and linked to neurodegenerative/psychiatric diseases. An eight-amino-acid peptide snippet of ADNP, NAP (NAPVSIPQ), identified as a smallest active fragment, includes the SxIP microtubule (MT) end-binding protein (EB) association motif, and enhances ADNP-EB3 interaction. Depletion of EB1 or EB3 abolishes NAP protection against zinc intoxication. Furthermore, NAP enhances Tau-MT interaction, and Tau regulates the localization and function of EB1 and EB3 in developing neuronal cells. Here, we asked how NAP (ADNP) enhances Tau-MT interactions and whether this is mediated by EBs. We showed, for we believe the first time, that NAP augmented endogenous EB1 comet density in the N1E-115 neuroblastoma neuronal model. This finding was substantiated by cell transfection with fluorescent EB1 and live cell imaging. NAP increased comet amounts, length and speed. At the molecular level, NAP enhanced EB3 homodimer formation, while decreasing EB1-EB3 heterodimer content and driving EB1- and EB3-Tau interactions (dramatic 20-fold increases), leading to recruitment of EB1/EB3 and Tau to MTs under zinc intoxication. Our previous results showed that while NAP protected neuronal-like cells against oxidative stress, it did not protect NIH3T3 fibroblasts. Here, NAP did not protect NIH3T3 cells against zinc intoxication, unless these cells were transfected with Tau. Interestingly, other MT associated proteins (MAPs) may replace Tau, thus, EB-Tau (MAPs) interaction is identified as a novel target for endogenous ADNP neuroprotection, and a future target for drug development, with NAP as a prototype.Molecular Psychiatry advance online publication, 24 January 2017; doi:10.1038/mp.2016.255.

  4. The Arecibo Observatory Space Academy

    NASA Astrophysics Data System (ADS)

    Rodriguez-Ford, Linda A.; Fernanda Zambrano Marin, Luisa; Aponte Hernandez, Betzaida; Soto, Sujeily; Rivera-Valentin, Edgard G.

    2016-10-01

    The Arecibo Observatory Space Academy (AOSA) is an intense fifteen-week pre-college research program for qualified high school students residing in Puerto Rico, which includes ten days for hands-on, on site research activities. Our mission is to prepare students for their professional careers by allowing them to receive an independent and collaborative research experience on topics related to the multidisciplinary field of space science. Our objectives are to (1) supplement the student's STEM education via inquiry-based learning and indirect teaching methods, (2) immerse students in an ESL environment, further developing their verbal and written presentation skills, and (3) foster in every student an interest in the STEM fields by harnessing their natural curiosity and knowledge in order to further develop their critical thinking and investigation skills. Students interested in participating in the program go through an application, interview and trial period before being offered admission. They are welcomed as candidates the first weeks, and later become cadets while experiencing designing, proposing, and conducting research projects focusing in fields like Physics, Astronomy, Geology, Chemistry, and Engineering. Each individual is evaluated with program compatibility based on peer interaction, preparation, participation, and contribution to class, group dynamics, attitude, challenges, and inquiry. This helps to ensure that specialized attention can be given to students who demonstrate a dedication and desire to learn. Deciding how to proceed in the face of setbacks and unexpected problems is central to the learning experience. At the end of the semester, students present their research to the program mentors, peers, and scientific staff. This year, AOSA students also focused on science communication and were trained by NASA's FameLab. Students additionally presented their research at this year's International Space Development Conference (ISDC), which was held in

  5. The Press Research Centre, 1956-1976.

    ERIC Educational Resources Information Center

    Press Research Centre, Krakow (Poland).

    In 1956, the Press Research Centre was established in Cracow, Poland by a group of journalists and publishers, for the purpose of instituting press research that would have practical applications. The aims of the Centre were to conduct studies on the history of the Polish press, the contemporary press, press readership, and editorial techniques.…

  6. National Academies-Review of the Research Program of the FreedomCAR and Fuel Partnership. Third Report

    SciTech Connect

    none,

    2011-06-09

    This report by the National Research Council's (NRC's) Committee on Review of the FreedomCAR and Fuel Research Program, Phase 3, is the third NRC review. The Phase 1 and Phase 2 reviews were issued in 2005 and 2008, respectively (NRC, 2005, 2008). The long-range goals of the Partnership focus on a transition to a highway transportation system that uses sustainable energy resources and reduces emissions, including net carbon emissions, on a life-cycle or well (source)-to-wheels basis (DOE, 2004). The Partnership focuses on precompetitive research and development (R&D) that can help to accelerate the emergence of technologies that can meet the long-range goals. • This review document is published by National Academies Press. You may (a) read the text for free on the National Academies Press web site, (b) download a free PDF after providing some identifying information, or (c) purchase a paperback copy of the book.

  7. Effects of daytime naps on procedural and declarative memory in patients with schizophrenia.

    PubMed

    Seeck-Hirschner, Mareen; Baier, Paul Christian; Sever, Serap; Buschbacher, Andrea; Aldenhoff, Josef B; Göder, Robert

    2010-01-01

    Sleep has been identified as a state that optimizes the consolidation of newly acquired information in memory. Straight memory deficits and sleep disturbances are well-known in patients with schizophrenia. This study tested the hypothesis that patients with schizophrenia have a deficit in procedural and declarative memory consolidation after a short midday nap when compared to healthy controls and patients with remitted to moderate major depression. Following a normal night's sleep, 22 healthy subjects, 20 patients with major depression and 21 patients with schizophrenia were studied in a napping and wake condition in a random-order cross-over design, early in the afternoon. To test declarative memory, the Rey-Osterrieth Complex Figure Test respectively the Taylor Complex Figure Test and, for procedural learning, a mirror tracing task were performed. The present study is the first to demonstrate significant differences between individuals with schizophrenia, depression and healthy matched controls with regard to measures of sleep and memory performance after a short period of daytime sleep (napping). In particular we found that a daytime nap of only about 40min led to improvement of declarative memory performance in all investigated groups, whereas no beneficial effect was seen on procedural performance in the group of medicated patients with schizophrenia in contrast to healthy controls and patients with remitted to moderate major depression.

  8. Performance Sustainment of Two Man Crews during 87 Hours of Extended Wakefulness with Stimulants and Napping

    DTIC Science & Technology

    2009-02-01

    normalized) data ............................................................................................18  5. Time spent in each stage of sleep...during the 2-hour nap. .........................................................19  6. Time spent in each stage of sleep during recovery sleep...contralateral mastoid. The subjects were allowed to remain in bed either until 20 minutes had elapsed or until he/she entered stage 2 sleep (the first K

  9. NAP-1, Nucleosome assembly protein 1, a histone chaperone involved in Drosophila telomeres.

    PubMed

    López-Panadès, Elisenda; Casacuberta, Elena

    2016-03-01

    Telomere elongation is a function that all eukaryote cells must accomplish in order to guarantee, first, the stability of the end of the chromosomes and second, to protect the genetic information from the inevitable terminal erosion. The targeted transposition of the telomere transposons HeT-A, TART and TAHRE perform this function in Drosophila, while the telomerase mechanism elongates the telomeres in most eukaryotes. In order to integrate telomere maintenance together with cell cycle and metabolism, different components of the cell interact, regulate, and control the proteins involved in telomere elongation. Different partners of the telomerase mechanism have already been described, but in contrast, very few proteins have been related with assisting the telomere transposons of Drosophila. Here, we describe for the first time, the implication of NAP-1 (Nucleosome assembly protein 1), a histone chaperone that has been involved in nuclear transport, transcription regulation, and chromatin remodeling, in telomere biology. We find that Nap-1 and HeT-A Gag, one of the major components of the Drosophila telomeres, are part of the same protein complex. We also demonstrate that their close interaction is necessary to guarantee telomere stability in dividing cells. We further show that NAP-1 regulates the transcription of the HeT-A retrotransposon, pointing to a positive regulatory role of NAP-1 in telomere expression. All these results facilitate the understanding of the transposon telomere maintenance mechanism, as well as the integration of telomere biology with the rest of the cell metabolism.

  10. [Association between insomnia symptoms, daytime napping, and falls in community-dwelling elderly].

    PubMed

    Pereira, Alexandre Alves; Ceolim, Maria Filomena; Neri, Anita Liberalesso

    2013-03-01

    This study focused on associations between insomnia symptoms, daytime napping, and falls in community-dwelling elderly, using a population-based cross-sectional design and probability sample with 689 community-dwelling elders. The protocol consisted of self-reported and physical performance variables. The study used univariate and multivariate logistic regression analysis with statistical significance set at p < 0.05. Prevalence rates for insomnia symptoms and daytime napping were 49.9% (n = 339) and 62.8% (n = 432), respectively. 14.4% reported a single fall and 11.9% reported multiple falls. Falls were associated with female gender (OR = 7.73; 95%CI: 3.03-19.72), age > 80 (OR = 3.48; 95%CI: 1.54-7.85), napping (OR = 2.24; 95%CI: 1.24-4.05), and depressive symptoms (OR = 1.98; 95%CI: 1.11-3.53). The association between daytime napping and falls corroborates data from international research. Identifying modifiable risk factors may help programs to prevent falls in the elderly.

  11. Structure and immunomodulatory property relationship in NapA of Borrelia burgdorferi.

    PubMed

    Codolo, Gaia; Papinutto, Elena; Polenghi, Alessandra; D'Elios, Mario Milco; Zanotti, Giuseppe; de Bernard, Marina

    2010-12-01

    NapA from Borrelia burgdorferi is a member of the Dps-like protein family with specific immunomodulatory properties; in particular, NapA is able to induce the expression of IL-23 in neutrophils and monocytes, as well as the expression of IL-6, IL-1β, and transforming growth factor beta (TGF-β) in monocytes, via Toll-like receptor (TLR) 2. Such an activity on innate immune cells triggers a synovial fluid Th17 response. Here we report the crystal structure of NapA, determined at 2.6Å resolution, which shows that the quaternary structure of the protein is that of a dodecamer with 23 symmetry, typical of the proteins of the family. We also demonstrate that the N- and C-terminal tails, which are flexible and not visible in the crystal, are not relevant for its pro-Th17 activity. Based on the crystal structure and on the comparison with the structure of the orthologous protein from Helicobacter pylori, HP-NAP, we hypothesize that the charge distributions on the two proteins' surfaces are responsible for the interaction with TLR2 and for the different behaviors in modulating the immune response.

  12. The Effects of Morning Naps, Car Trips, and Maternal Separation on Adrenocortical Activity in Human Infants.

    ERIC Educational Resources Information Center

    Larson, Mary C.; And Others

    1991-01-01

    Three studies examined adrenocortical activity in infants. Morning naps were associated with decreases in salivary cortisol. Riding for 40 minutes in a car lowered salivary cortisol concentrations. Thirty minutes of maternal separation in the laboratory resulted in higher salivary cortisol concentrations than did 30 minutes of play with the mother…

  13. Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion.

    PubMed

    Fang, Guoliang; Zhang, Dachuan; Yin, Huilong; Zheng, Lu; Bi, Xiaolin; Yuan, Li

    2014-04-15

    Centrosome cohesion, mostly regarded as a proteinaceous linker between parental centrioles, ensures that the interphase centrosome(s) function as a single microtubule-organizing center. Impairment of centrosome cohesion leads to the splitting of centrosomes. Although the list of cohesion proteins is growing, the precise composition and regulation of centrosome cohesion are still largely unknown. In this study, we show that the centriolar protein centlein (also known as CNTLN) localizes to the proximal ends of the centrioles and directly interacts with both C-Nap1 (also known as Cep250) and Cep68. Moreover, centlein complexes with C-Nap1 and Cep68 at the proximal ends of centrioles during interphase and functions as a molecular link between C-Nap1 and Cep68. Depletion of centlein impairs recruitment of Cep68 to the centrosomes and, in turn, results in centrosome splitting. Both centlein and Cep68 are novel Nek2A substrates. Collectively, our data demonstrate that centrosome cohesion is maintained by the newly identified complex of C-Nap1-centlein-Cep68.

  14. A comparison of impulse drying to double felted pressing on pilot- scale shoe presses and roll presses

    SciTech Connect

    Orloff, D.I.

    1992-08-01

    Pilot-scale shoe press and roll press experiments have been conducted to compare impulse drying and double felted pressing. Both ceramic coated and Beloit Type C press rolls have been evaluated. The experiments show that impulse drying can provide significantly higher outgoing solids than double felled pressing at the same impulse. For example, at an impulse of 0.234 MPa seconds (34 psi seconds), sheets at an ingoing solids of 52% were impulse dried (using the Beloit Type C press roll) to 68% solids while optimized double felled pressing could only yield press dryness of, at most, 60%.

  15. High School Teaching Career Academies: Profiles and Practices. Urban Teacher Academy Project.

    ERIC Educational Resources Information Center

    Council of the Great City Schools, Washington, DC.

    The Urban Teacher Academy Project (UTAP) promotes the recruitment and preparation of qualified and diverse teachers for urban schools by expanding a school-to-career approach through high school teaching career academies. The UTAP has documented the best practices and developed practical information to guide the formation of career teaching…

  16. Novel tubulin and tau neuroprotective fragments sharing structural similarities with the drug candidate NAP (Davuentide).

    PubMed

    Gozes, Illana; Iram, Tal; Maryanovsky, Evgenia; Arviv, Carmit; Rozenberg, Liora; Schirer, Yulie; Giladi, Eliezer; Furman-Assaf, Sharon

    2014-01-01

    NAP (NAPVSIPQ, davunetide) is a microtubule stabilizing peptide drug candidate. Here, we set out to identify NAP-like peptides that provide neuroprotection and reduce tau pathology. NAP-like peptides were derived using publically available search engines, which identified sequence homologies in the microtubule subunit tubulin and in the microtubule associated protein, tau. NATLSIHQ (NAT) and STPTAIPQ were derived from tubulin, and TAPVPMPD (TAP) was derived from tau. All peptides provided neuroprotection against the Alzheimer's disease (AD) toxin, the amyloid-β 1-42 peptide, although NAT and TAP were much more potent than STPTAIPQ. NAT also protected astrocytes, while STPTAIPQ was active only at micromolar concentrations. Because NAT and TAP were much more potent than STPTAIPQ in neuroprotection, those peptides were also tested for inhibition of tau-like aggregation (the second protein hallmark pathology of AD). Both NAT and TAP inhibited tau-like aggregation, with NAT being active over a very broad concentration range. NAT also protected in vivo in a frontotemporal dementia transgenic mouse model (Tau-Tg), when tested at the age of ~10 months. Results showed significantly decreased levels of the NAP parent protein, activity-dependent neuroprotective protein in the cerebral cortex of the Tau-Tg which was increased back to normal levels by NAT treatment. This was coupled to protection of Brain-Body weight ratio in the compromised Tau-Tg. With AD being the major tauopathy and with tau taking part in frontotemporal dementia, novel NAP derivatives that reduce tauopathy and provide neuroprotection are of basic and clinical interest.

  17. Napping in older people 'at risk' of dementia: relationships with depression, cognition, medical burden and sleep quality.

    PubMed

    Cross, Nathan; Terpening, Zoe; Rogers, Naomi L; Duffy, Shantel L; Hickie, Ian B; Lewis, Simon J G; Naismith, Sharon L

    2015-10-01

    Sleep disturbance is prevalent in older adults, particularly so in those at a greater risk of dementia. However, so far the clinical, medical and neuropsychological correlates of daytime sleep have not been examined. The aims of this study were to investigate the characteristics and effects of napping using actigraphy in older people, particularly in those 'at risk' of dementia. The study used actigraphy and sleep diaries to measure napping habits in 133 older adults 'at risk' of dementia (mean age = 65.5 years, SD = 8.4 years), who also underwent comprehensive medical, psychiatric and neuropsychological assessment. When defined by actigraphy, napping was present in 83.5% (111/133) of participants; however, duration and timing varied significantly among subjects. Nappers had significantly greater medical burden and body mass index, and higher rates of mild cognitive impairment. Longer and more frequent naps were associated with poorer cognitive functioning, as well as higher levels of depressive symptoms, while the timing of naps was associated with poorer nocturnal sleep quality (i.e. sleep latency and wake after sleep onset). This study highlights that in older adults 'at risk' of dementia, napping is associated with underlying neurobiological changes such as depression and cognition. Napping characteristics should be more routinely monitored in older individuals to elucidate their relationship with psychological and cognitive outcomes.

  18. STS-102 Prelaunch Press Conference

    NASA Technical Reports Server (NTRS)

    2001-01-01

    Joel Wells, NASA Public Affairs, introduces Ron Dittemore, NASA Shuttle Program Manager, Tommy Holloway, NASA International Space Station Program Manager, Dave King, NASA Director of Shuttle Processing, and Captain Clif Stargardt, US Air Force Meteorologist, in this STS-102 prelaunch press conference. The men give an overview of the prelaunch processing for the Discovery Orbiter (such as the PRSD loading) and give a weather forecast for launch. They then answer questions from the press.

  19. Protection against tauopathy by the drug candidates NAP (davunetide) and D-SAL: biochemical, cellular and behavioral aspects.

    PubMed

    Shiryaev, Natalia; Pikman, Regina; Giladi, Eliezer; Gozes, Illana

    2011-01-01

    Activity-dependent neuroprotective protein (ADNP) is essential for brain formation and partial deficiency in ADNP results in cognitive deficits coupled with tauopathy and neuronal cell death. Our previous results indicated that a peptide snippet from ADNP, NAPVSIPQ (NAP, generic name, davunetide) can restore in part ADNP deficiencies. NAP interacts with tubulin and this interaction is displaced by the NAP related peptide that is derived from activity-dependent neurotrophic factor (ADNF), SALLRSIPA (SAL) and its all D-amino acid peptide derivative (D-SAL, also known as AL-309). Both NAP and D-SAL were shown to protect neurons against amyloid beta toxicity however the mechanism of protection is still under investigation. In addition, NAP protects against tau hyperphosphorylation associated with ADNP deficiency, in vivo. To investigate whether the mechanism of in vitro neuroprotection relates to the in vivo protection against tauopathy and to draw potential additional parallelism between NAP and D-SAL, we asked if: 1]NAP and D-SAL protect against amyloid beta related tau hyperphosphorylation in vitro; and 2] D-SAL protects against haploinsufficiency in ADNP, inhibiting tauopathy in vivo. Assessment of NAP and D-SAL neuroprotection in primary cortical neuro-glial cultures treated with amyloid beta showed that both peptides reduced toxin-related neuronal damage and protected against tau hyperphosphorylation. In vivo, chronic D-SAL administration protected against tau hyperphosphorylation associated with ADNP deficiency (ADNP+/- mice), showing for the first time protection against deficits in odor discrimination and in social recognition. These studies associate neuroprotection in vivo and in vitro and provide a broad base for future drug development based on NAP and D-SAL against multiple neurodegenerative conditions.

  20. The Bradyrhizobium japonicum napEDABC genes are controlled by the FixLJ-FixK(2)-NnrR regulatory cascade.

    PubMed

    Robles, E F; Sánchez, C; Bonnard, N; Delgado, M J; Bedmar, E J

    2006-02-01

    Nitrate respiration by the N(2)-fixing symbiotic bacteria Bradyrhizobium japonicum USDA110 is mediated by a Nap (periplasmic nitrate reductase) encoded by the napEDABC genes. Expression of a transcriptional fusion of the nap promoter region to the reporter gene lacZ, P(napE)-lacZ, was very low in aerobically grown cells of USDA110, but expression was induced approx. 3-fold when the cells were cultured under microaerobic conditions, and 12-fold when nitrate was added to the microaerobic incubation medium. The P(napE)-lacZ fusion was not expressed in the fixL 7403, fixJ 7360 and fixK(2) 9043 mutant strains. Microaerobic induction of the P(napE)-lacZ fusion was retained in the nnrR 8678 mutant, but no increase in beta-galactosidase activity was observed upon nitrate addition. Western-blot and Methyl Viologen-dependent nitrate reductase activity assays showed that synthesis and activity of the catalytic NapA subunit in USDA110 was similar to that in the napC 0906 and nirK GRK308 mutant strains incubated microaerobically with nitrate. These results suggest that nitrate and nitrite, which are not reduced by the napC 0906 and nirK GRK308 mutant cells respectively, induced the synthesis and activity of NapA; conversely, formation of endogenous NO was not required for induction of Nap expression.

  1. J-curve relation between daytime nap duration and type 2 diabetes or metabolic syndrome: A dose-response meta-analysis.

    PubMed

    Yamada, Tomohide; Shojima, Nobuhiro; Yamauchi, Toshimasa; Kadowaki, Takashi

    2016-12-02

    Adequate sleep is important for good health, but it is not always easy to achieve because of social factors. Daytime napping is widely prevalent around the world. We performed a meta-analysis to investigate the association between napping (or excessive daytime sleepiness: EDS) and the risk of type 2 diabetes or metabolic syndrome, and to quantify the potential dose-response relation using cubic spline models. Electronic databases were searched for articles published up to 2016, with 288,883 Asian and Western subjects. Pooled analysis revealed that a long nap (≥60 min/day) and EDS were each significantly associated with an increased risk of type 2 diabetes versus no nap or no EDS (odds ratio 1.46 (95% CI 1.23-1.74, p < 0.01) for a long nap and 2.00 (1.58-2.53) for EDS). In contrast, a short nap (<60 min/day) was not associated with diabetes (p = 0.75). Dose-response meta-analysis showed a J-curve relation between nap time and the risk of diabetes or metabolic syndrome, with no effect of napping up to about 40 minutes/day, followed by a sharp increase in risk at longer nap times. In summary, longer napping is associated with an increased risk of metabolic disease. Further studies are needed to confirm the benefit of a short nap.

  2. J-curve relation between daytime nap duration and type 2 diabetes or metabolic syndrome: A dose-response meta-analysis

    PubMed Central

    Yamada, Tomohide; Shojima, Nobuhiro; Yamauchi, Toshimasa; Kadowaki, Takashi

    2016-01-01

    Adequate sleep is important for good health, but it is not always easy to achieve because of social factors. Daytime napping is widely prevalent around the world. We performed a meta-analysis to investigate the association between napping (or excessive daytime sleepiness: EDS) and the risk of type 2 diabetes or metabolic syndrome, and to quantify the potential dose-response relation using cubic spline models. Electronic databases were searched for articles published up to 2016, with 288,883 Asian and Western subjects. Pooled analysis revealed that a long nap (≥60 min/day) and EDS were each significantly associated with an increased risk of type 2 diabetes versus no nap or no EDS (odds ratio 1.46 (95% CI 1.23–1.74, p < 0.01) for a long nap and 2.00 (1.58–2.53) for EDS). In contrast, a short nap (<60 min/day) was not associated with diabetes (p = 0.75). Dose-response meta-analysis showed a J-curve relation between nap time and the risk of diabetes or metabolic syndrome, with no effect of napping up to about 40 minutes/day, followed by a sharp increase in risk at longer nap times. In summary, longer napping is associated with an increased risk of metabolic disease. Further studies are needed to confirm the benefit of a short nap. PMID:27909305

  3. Expression of peptide NAP in rat retinal Müller cells prevents hypoxia-induced retinal injuries and promotes retinal neurons growth.

    PubMed

    Zheng, Yuping; Zeng, Hao; She, Huaning; Liu, Hui; Sun, Naixue

    2010-07-01

    NAP (NAPVSIPQ) is a short peptide derived from activity-dependent neuroprotective protein (ADNP) sequence, whose potent and direct neuroprotective capabilities have been widely accepted. However, due to the high risk and inconvenience of intraocular injections, NAP is difficult to be clinically administered as therapeutic agent in treating retinal diseases. Currently, stable transfection of this octapeptide into cells has not been reported, partly because of its small size and lacking of 5' signal sequence. Here, we have developed a novel NT4-NAP fusion gene by attaching the 5' nonfunctional preproregion of neurotrophin 4 (NT4) to NAP cDNA. Recombinant adeno-associated virus was established to introduce NT4-NAP construct into cultured rat retinal Müller cells (RMC), resulting in sustained high level NAP production from stable transfection. Functional analyses of RMC cells transfected with NAP revealed the remarkably reduced cytotoxicity and apoptosis of the cells under hypoxia. Furthermore, coculturing of transfected RMC-NAP cells with primary rat retinal neural cells offer marked protection to the latter against hypoxia induced cellular damages. Together our data indicate that stable transfection of NAP into retinal Müller cells with constant NAP production is possible. NAP produced from cellular transfection maintained its biological neuroprotective activities. This targeted gene expression may provide an effective treatment for retinal diseases in the near future.

  4. Automatic estimation of sleep level for nap based on conditional probability of sleep stages and an exponential smoothing method.

    PubMed

    Wang, Bei; Wang, Xingyu; Zhang, Tao; Nakamura, Masatoshi

    2013-01-01

    An automatic sleep level estimation method was developed for monitoring and regulation of day time nap sleep. The recorded nap data is separated into continuous 5-second segments. Features are extracted from EEGs, EOGs and EMG. A parameter of sleep level is defined which is estimated based on the conditional probability of sleep stages. An exponential smoothing method is applied for the estimated sleep level. There were totally 12 healthy subjects, with an averaged age of 22 yeas old, participated into the experimental work. Comparing with sleep stage determination, the presented sleep level estimation method showed better performance for nap sleep interpretation. Real time monitoring and regulation of nap is realizable based on the developed technique.

  5. 7 CFR 550.29 - Press releases.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Press releases. 550.29 Section 550.29 Agriculture... Program Management § 550.29 Press releases. Press releases or other forms of public notification will be... opportunity to review, in advance, all written press releases and any other written information to be...

  6. 75 FR 44276 - National Fire Academy Board of Visitors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-28

    ... SECURITY Federal Emergency Management Agency National Fire Academy Board of Visitors AGENCY: Federal Emergency Management Agency, DHS. ACTION: Notice of cancellation of meeting. SUMMARY: The National Fire... FR 39561) announcing a National Fire Academy Board of Visitors public teleconference meeting...

  7. Integrating nap and night-time sleep into sleep patterns reveals differential links to health-relevant outcomes.

    PubMed

    Devine, Jaime K; Wolf, Jutta M

    2016-04-01

    Both night-time sleep and nap behaviour have been linked consistently to health outcomes. Although reasons for napping are usually tied to night-time sleep, the majority of studies assess their effects independently. The current study thus aimed to examine the health relevance of patterns of sleep behaviour that take into account both night-time and daytime sleep habits. Night-time sleep, recorded during 7 days via actigraphy from 313 participants (aged 34-82 years) of the Midlife in the United States II Biomarker study, was assessed. Blood and urine specimens were assayed for noradrenaline, interleukin-6 and C-reactive protein. Participants self-reported nap behaviour, depressive symptoms, perceived chronic stress and the presence of medical symptoms and conditions. Overall, nappers (n = 208) showed elevated waist-hip ratios, C-reactive protein and interleukin-6 levels compared to non-nappers and reported more physiological symptoms and conditions (all P ≤ 0.019). Within nappers, cluster analysis revealed three patterns of sleep behaviour-infrequent nappers with good night-time sleep, frequent nappers with good night-time sleep and nappers with poor night-time sleep. Nappers with poor night-time sleep thereby exhibited elevated noradrenaline levels, depressive symptoms and perceived stress scores compared to other groups (all P ≤ 0.041). These findings support the idea that nap-health relationships are complex, in that frequency of napping and accumulation of nap sleep is not related linearly to health consequences. Assessing nap behaviour in conjunction with night-time sleep behaviour appeared crucial to elucidate further the health relevance of napping, particularly in terms of psychological health outcomes, including chronic stress and depressive symptoms.

  8. NAP (davunetide) modifies disease progression in a mouse model of severe neurodegeneration: protection against impairments in axonal transport.

    PubMed

    Jouroukhin, Yan; Ostritsky, Regina; Assaf, Yaniv; Pelled, Galit; Giladi, Eliezer; Gozes, Illana

    2013-08-01

    NAP (davunetide) is a novel neuroprotective compound with mechanism of action that appears to involve microtubule (MT) stabilization and repair. To evaluate, for the first time, the impact of NAP on axonal transport in vivo and to translate it to neuroprotection in a severe neurodegeneration, the SOD1-G93A mouse model for amyotrophic lateral sclerosis (ALS) was used. Manganese-enhanced magnetic resonance imaging (MRI), estimating axonal transport rates, revealed a significant reduction of the anterograde axonal transport in the ALS mice compared to healthy control mice. Acute NAP treatment normalized axonal transport rates in these ALS mice. Tau hyperphosphorylation, associated with MT dysfunction and defective axonal transport, was discovered in the brains of the ALS mice and was significantly reduced by chronic NAP treatment. Furthermore, in healthy wild type (WT) mice, NAP reversed axonal transport disruption by colchicine, suggesting drug-dependent protection against axonal transport impairment through stabilization of the neuronal MT network. Histochemical analysis showed that chronic NAP treatment significantly protected spinal cord motor neurons against ALS-like pathology. Sequential MRI measurements, correlating brain structure with ALS disease progression, revealed a significant damage to the ventral tegmental area (VTA), indicative of impairments to the dopaminergic pathways relative to healthy controls. Chronic daily NAP treatment of the SOD1-G93A mice, initiated close to disease onset, delayed degeneration of the trigeminal, facial and hypoglossal motor nuclei as was significantly apparent at days 90-100 and further protected the VTA throughout life. Importantly, protection of the VTA was significantly correlated with longevity and overall, NAP treatment significantly prolonged life span in the ALS mice.

  9. Common Ground. Feminist Collaboration in the Academy.

    ERIC Educational Resources Information Center

    Peck, Elizabeth G., Ed.; Mink, JoAnna Stephens, Ed.

    The fifteen articles in this anthology examine the process of collaboration as it fits into questions of gender. Articles include: "Educate, Organize, and Agitate: A Historical Overview of Feminist Collaboration in Great Britain and America, 1640-1930" (Melodie Andrews); "Beyond Feminism: An Intercultural Challenge for Transforming the Academy"…

  10. The Academy for Career Education: Diffusion Issues.

    ERIC Educational Resources Information Center

    Maguire, Louis M.

    A model experience-based career education program for secondary school students in Philadelphia, called the Academy for Career Education, has been operated by Research for Better Schools, Inc. (RBS), for three years. It utilizes employer/community participants as instructional agents and learning sites for career exploration and specialization,…

  11. Leadership Academies: A District Office Perspective

    ERIC Educational Resources Information Center

    Doll, Rick

    2016-01-01

    This article investigates district-level administrators' perceptions regarding the value of the partnership academies. The article uses input from seven district administrators who provided feedback regarding the value of the district and university partnership, specific benefits to the district, the differences between participants who envision…

  12. Corporate Features and Faith-Based Academies

    ERIC Educational Resources Information Center

    Green, Elizabeth

    2009-01-01

    This article forms an introductory exploration into the relationship between corporate features and religious values in Academies sponsored by a Christian foundation. This is a theme which arose from research comprising the ethnography of a City Technology College (CTC) with a Christian ethos. The Christian foundation which sponsors the CTC also…

  13. Can Academic Freedom Work in Military Academies?

    ERIC Educational Resources Information Center

    Niday, Jackson A., II; Harrington, Kathleen

    2007-01-01

    This article addresses the thorny question of military discipline vs. academic freedom, demonstrating that the military academy is the perfect proving ground if you want to test the value of academic freedom. The authors, who are also Air Force officers, embarked on a two-year quest to determine what place academic freedom has at a military…

  14. Broad Academy's Growing Reach Draws Scrutiny

    ERIC Educational Resources Information Center

    Samuels, Christina A.

    2011-01-01

    Billionaire businessman Eli Broad, one of the country's most active philanthropists, founded the "Broad Superintendents Academy" in 2002 with an extraordinarily optimistic goal: Find leaders from both inside and outside education, train them, and have them occupying the superintendencies in a third of the 75 largest school districts--all in just…

  15. Lost Leaders: Women in the Global Academy

    ERIC Educational Resources Information Center

    Morley, Louise

    2014-01-01

    Drawing on data gathered from British Council seminars in Hong Kong, Tokyo and Dubai on "Absent Talent: Women in Research and Academic Leadership" (2012-2013), this paper discusses academic women's experiences and explanations for women's under-representation as knowledge leaders and producers in the global academy. Participants from…

  16. Researchers fear 'Putin's Academy of Sciences'

    NASA Astrophysics Data System (ADS)

    Moskvitch, Katia

    2013-11-01

    Scientists have voiced concerns about the future of the Russian Academy of Sciences (RAS) after the country's president, Vladimir Putin, signed a law that will make the 289-year-old body come under the direct control of a new government agency.

  17. Freedoms and Perils: Academy Schools in England

    ERIC Educational Resources Information Center

    Heilbronn, Ruth

    2016-01-01

    Can Dewey's Moral Principles in Education throw light on a contemporary policy issue in education, namely the privatisation of education through the establishment of academy schools in England? The article first considers what the policy entails, in terms of its conception of education as a market commodity. The next section suggests an…

  18. How Academies Threaten the Comprehensive Curriculum

    ERIC Educational Resources Information Center

    Titcombe, Roger

    2008-01-01

    The Freedom of Information Act (FOI) was used over a three-year period to investigate the curriculum of state schools and academies. The resulting data has shown that spectacular apparent school improvement, in terms of five or more A*-C GCSE /GNVQ passes has been largely brought about by the substitution of mainstream curriculum subjects by much…

  19. Action Learning Drives the Emerald Academy

    ERIC Educational Resources Information Center

    Nalborczyk, Sarah; Sandelands, Luke

    2012-01-01

    This account examines the action learning process adopted by Emerald Group Publishing Ltd., embedded in the organization through the in-company Emerald Academy. In case study format, the paper emphasizes that in order to align learning with organizational objectives joined up thinking and practice is needed beyond the learning and development…

  20. An Urban Academy for Inner City Youth.

    ERIC Educational Resources Information Center

    Shane, Ruth S.

    1989-01-01

    Franklyn G. Jenifer, Chancellor of the Board of Regents of Higher Education, Commonwealth of Massachusetts, proposes residential urban schools for at-risk inner city youth. These "urban academies" would remove those who are most vulnerable from the destructive ghetto environment and totally involve them in the educational environment. (FMW)

  1. District Liaison Involvement in Partnership Academies

    ERIC Educational Resources Information Center

    Gustafson, Debra M.; Kiltz, Nancy

    2016-01-01

    In this article, Gustafson and Kiltz reflect on why they got involved in the very first master's level Professional Administrative Leadership Academy developed at Kansas State University (KSU). The authors had the opportunity to observe school leaders and noted that the most effective leaders shared their power and knowledge freely with others.…

  2. A Guide to the Evidence on Academies

    ERIC Educational Resources Information Center

    Sims, D.; Grayson, H.; Wespieser, K.

    2015-01-01

    Academic schools are a prominent feature of the educational landscape in England. Introduced in 2002, they are funded directly from central government and have become a major policy driver to reform the school system and increase educational attainment. Academies count for 60 percent of secondary schools and 13 percent of primaries. This report…

  3. Core Works Program: The Montclair Kimberley Academy.

    ERIC Educational Resources Information Center

    Montclair Kimberley Academy, NJ.

    This program lists texts, music, and art works that have been selected as a core of studies that continue through the curriculum from pre-kindergarten through 12th grade at The Montclair Kimberley Academy (New Jersey). The selections are representative of important texts, music, and art that have been taught to the Montclair Kimberley Academy…

  4. Lafayette Academy Charter School: Safe and Predictable

    ERIC Educational Resources Information Center

    Principal Leadership, 2012

    2012-01-01

    This article features Lafayette Academy Charter School in New Orleans, Louisiana, which was recognized by the state board of education as a "center of excellence" in 2010, and given an automatic renewal of its charter--the only school in the state to receive this honor. The school has demonstrated impressive progress: the 2007 test…

  5. Secrets of the STEM X Academy

    ERIC Educational Resources Information Center

    Teaching Science, 2016

    2016-01-01

    Science teachers, whether in primary or secondary schools, are in the front line for creating a more scientifically literate community. This article describes the STEM X Academy, a program created with the following goals: (1) demystify Science, Technology, Engineering, and Mathematics (STEM); (2) help teachers transform their teaching with…

  6. Hand's Academy Challenge: Some Starter Questions

    ERIC Educational Resources Information Center

    Gardner, Peter

    2014-01-01

    Michael Hand has recently challenged certain religious organisations that run Academies in the United Kingdom to devise and pursue their own faith-based curricula in their schools. In this short article I examine some of the problems Hand's challenge might encounter, including whether religious conceptions of worthwhile activities and of human…

  7. General Education at the Coast Guard Academy.

    ERIC Educational Resources Information Center

    Sanders, William A.

    In seeking the most effective presentation of the liberal arts in curricula such as the heavily technical and professional curricula at the U.S. Coast Guard Academy, which leave little room for general education, general education course design must capture the imagination of students and motivate them for continuing self-education. Development of…

  8. Audience Perceptions of the Academy Awards Telecast.

    ERIC Educational Resources Information Center

    Real, Michael R.; Hassett, Christopher A.

    Residents in a southern California city were surveyed by telephone about their attitudes toward the telecast of the motion picture "Academy Awards" presentations. Of the 141 respondents, 71 had not watched the telecast, 42 had watched some of the broadcast, and 28 had watched all of it. Less than one third of the survey questions were specifically…

  9. Jupiter Environmental Research & Field Studies Academy.

    ERIC Educational Resources Information Center

    Huttemeyer, Bob

    1996-01-01

    Describes the development and workings of the Jupiter Environmental Research and Field Studies Academy that focuses on enabling both teachers and students to participate in real-life learning experiences. Discusses qualifications for admittance, curriculum, location, ongoing projects, students, academics, preparation for life, problem solving, and…

  10. Analysing Religion and Education in Christian Academies

    ERIC Educational Resources Information Center

    Green, Elizabeth

    2012-01-01

    This paper asserts that the religious assumptions of Christian academies need to be fully examined in relation to any analysis of their cultural practices, impact or policy implications. It proposes that Bourdieu's concepts of habitus, cultural capital and symbolic power can be broadened out from their traditional use in accounting for social…

  11. [Peculiarities of medical backup of low flight course and nap-of-the-earth].

    PubMed

    blaginin, A A; Lizogub, I N; Sinel'niov, S N; Zhil'tsova, I I

    2014-07-01

    Authors presented peculiarities of low flight course and nap-of-the-earth: quick change of orientation cues, ground proximity, possible bird collision, loss of radio signal, exposure of oscillating acceleration, caused by increased atmosphere turbulence. It is showed that low flight course and nap-of-the-earth require special health status of pilots, who must be ready to evaluate obstacle distance, obstacle position relative to aircraft plane, gain in altitude, flight elevation and closing speed, new obstacles and make a decision to fly-by or turn. These circumstances cause emotional tension that affects working capacity and professional reliance. Air medical officer should take additional measures for medical backup of these flight courses.

  12. Changes in fractal structure of heart rate variability during a nap in one case.

    PubMed

    Shono, H; Shono, M; Takasaki, M; Iwasaka, T; Sugimori, H

    2001-06-01

    The objective of the present study was to analyze fractal structures of adult heart rate (HR) variability during a nap. Fractal analysis was carried out in one case over consecutive 10-min time series of HR, which were simultaneously recorded with electroencephalogram. Scaling relationships showed cross-over patterns characterized by alphas and alphal (i.e. slopes above and below a cross-over point). The alphas and alphal were black and white noise at Stage 4 of NREM sleep, and black and 1/f noise in REM sleep. Cross-over points changed from the first to second sleep cycle. We demonstrate the multifractal structures of HR variability during a nap in the present case.

  13. NAP-2 Secreted by Human NK Cells Can Stimulate Mesenchymal Stem/Stromal Cell Recruitment.

    PubMed

    Almeida, Catarina R; Caires, Hugo R; Vasconcelos, Daniela P; Barbosa, Mário A

    2016-04-12

    Strategies for improved homing of mesenchymal stem cells (MSCs) to a place of injury are being sought and it has been shown that natural killer (NK) cells can stimulate MSC recruitment. Here, we studied the chemokines behind this recruitment. Assays were performed with bone marrow human MSCs and NK cells freshly isolated from healthy donor buffy coats. Supernatants from MSC-NK cell co-cultures can induce MSC recruitment but not to the same extent as when NK cells are present. Antibody arrays and ELISA assays confirmed that NK cells secrete RANTES (CCL5) and revealed that human NK cells secrete NAP-2 (CXCL7), a chemokine that can induce MSC migration. Inhibition with specific antagonists of CXCR2, a receptor that recognizes NAP-2, abolished NK cell-mediated MSC recruitment. This capacity of NK cells to produce chemokines that stimulate MSC recruitment points toward a role for this immune cell population in regulating tissue repair/regeneration.

  14. NAP-2 Secreted by Human NK Cells Can Stimulate Mesenchymal Stem/Stromal Cell Recruitment

    PubMed Central

    Almeida, Catarina R.; Caires, Hugo R.; Vasconcelos, Daniela P.; Barbosa, Mário A.

    2016-01-01

    Summary Strategies for improved homing of mesenchymal stem cells (MSCs) to a place of injury are being sought and it has been shown that natural killer (NK) cells can stimulate MSC recruitment. Here, we studied the chemokines behind this recruitment. Assays were performed with bone marrow human MSCs and NK cells freshly isolated from healthy donor buffy coats. Supernatants from MSC-NK cell co-cultures can induce MSC recruitment but not to the same extent as when NK cells are present. Antibody arrays and ELISA assays confirmed that NK cells secrete RANTES (CCL5) and revealed that human NK cells secrete NAP-2 (CXCL7), a chemokine that can induce MSC migration. Inhibition with specific antagonists of CXCR2, a receptor that recognizes NAP-2, abolished NK cell-mediated MSC recruitment. This capacity of NK cells to produce chemokines that stimulate MSC recruitment points toward a role for this immune cell population in regulating tissue repair/regeneration. PMID:27052313

  15. Postpartum mothers' napping and improved cognitive growth fostering of infants: results from a pilot study.

    PubMed

    Ronzio, Cynthia R; Huntley, Edward; Monaghan, Maureen

    2013-04-01

    Little is known about how maternal sleep disturbances in the postpartum period affect mother-infant interaction. The study was designed to assess if less maternal sleep disturbance and less fatigue were associated with more positive mother-child interaction, independent of maternal depression symptoms. Twenty-three mothers completed 1 week of actigraphy and self-report measures of fatigue and depression symptoms. To determine the quality of mother-infant interaction, mothers and infants were then observed in a structured, video-recorded teaching episode. Greater maternal napping frequency was associated with better cognitive growth fostering of the infant (r s = .44, p < .05), a subscale of the interaction assessment. Maternal napping, not the quality or quantity of nocturnal sleep, appears to be associated with improvements in mother-infant interactions.

  16. Fatigue mitigation effects of en-route napping on commercial airline pilots flying international routes

    NASA Astrophysics Data System (ADS)

    Baldwin, Jarret Taylor

    The introduction of ultra-long range commercial aircraft and the evolution of the commercial airline industry has provided new opportunities for air carriers to fly longer range international route segments while deregulation, industry consolidation, and the constant drive to reduce costs wherever possible has pressured airline managements to seek more productivity from their pilots. At the same time, advancements in the understanding of human physiology have begun to make their way into flight and duty time regulations and airline scheduling practices. In this complex and ever changing operating environment, there remains an essential need to better understand how these developments, and other daily realities facing commercial airline pilots, are affecting their fatigue management strategies as they go about their rituals of getting to and from their homes to work and performing their flight assignments. Indeed, the need for commercial airline pilots to have access to better and more effective fatigue mitigation tools to combat fatigue and insure that they are well rested and at the top of their game when flying long-range international route segments has never been greater. This study examined to what extent the maximum fatigue states prior to napping, as self-accessed by commercial airline pilots flying international route segments, were affected by a number of other common flight assignment related factors. The study also examined to what extent the availability of scheduled en-route rest opportunities, in an onboard crew rest facility, affected the usage of en-route napping as a fatigue mitigation strategy, and to what extent the duration of such naps affected the perceived benefits of such naps as self-accessed by commercial airline pilots flying international route segments. The study utilized an online survey tool to collect data on crew position, prior flight segments flown in the same duty period, augmentation, commuting, pre-flight rest obtained in the

  17. Nap Interferes with Hypoxia-Inducible Factors and VEGF Expression in Retina of Diabetic Rats.

    PubMed

    D'Amico, Agata Grazia; Maugeri, Grazia; Bucolo, Claudio; Saccone, Salvatore; Federico, Concetta; Cavallaro, Sebastiano; D'Agata, Velia

    2017-02-01

    The retinal microvascular damage is a complication of diabetic retinopathy (DR). Hyperglycemia and hypoxia are responsible of aberrant vessel's proliferation. The cellular response to hypoxia is mediated through activation of hypoxia-inducible factors (HIFs). Among these, HIF-1α modulates expression of its target gene, VEGF, whose upregulation controls the angiogenic event during DR development. In a previous study, we have demonstrated that a small peptide, NAP, is able to protect retina from hyperglycemic insult. Here, we have demonstrated that its intraocular administration in a rat model of diabetic retinopathy has reduced expression of HIF-1α, HIF-2α, and VEGF by increasing HIF-3α levels. These data have been also confirmed by immunolocalization study by confocal microscopy. Although these evidences need to be further deepened to understand the molecular mechanism involved in the protective NAP action, the present data suggest that this small peptide may be effective to prevent the development of this ocular pathology.

  18. Nap1: taking a closer look at a juggler protein of extraordinary skills.

    PubMed

    Zlatanova, Jordanka; Seebart, Corrine; Tomschik, Miroslav

    2007-05-01

    The nucleosome assembly protein Nap1 is used extensively in the chromatin field to reconstitute nucleosomal templates for structural and functional studies. Beyond its role in facilitating experimental investigation of nucleosomes, the highly conserved Nap1 is one of the best-studied members of the histone chaperone group. Here we review its numerous functions, focusing mainly on its roles in assembly and disassembly of the nucleosome particle, and its interactions with chromatin remodeling factors. Its presumed role in transcription through chromatin is also reviewed in detail. An attempt is made to clearly discriminate between fact and fiction, and to formulate the unresolved questions that need further attention. It is beyond doubt that the numerous, seemingly unrelated functions of this juggler protein have to be precisely channeled, coordinated, and regulated. Why nature endowed this specific protein with so many functions may remain a mystery. We are aware of the enormous challenge to the scientific community that understanding the mechanisms underlying these activities presents.

  19. Apollo 13 Facts: Press Conference

    NASA Technical Reports Server (NTRS)

    2001-01-01

    Flight Director Gene Krantz gives an overview of the Apollo 13 mission as corrections are made in the power down checklist, passive thermal control, and orbital burns after the spacecraft runs into problems. He then answers questions from the press with the help of Tony England, Bill Peters, and Dick Thorson. Footage then shows newspaper headlines 'We're Not Concerned' and 'Getting Ready to Land' as people watch televisions to see if the astronauts landed safely. The press conference section of this video has sound, the headlines section does not.

  20. D-NAP prophylactic treatment in the SOD mutant mouse model of amyotrophic lateral sclerosis: review of discovery and treatment of tauopathy.

    PubMed

    Jouroukhin, Yan; Ostritsky, Regina; Gozes, Illana

    2012-11-01

    Davunetide (NAP) is a leading drug candidate being tested against tauopathy. Davunetide is an eight-amino-acid peptide fragment derived by structure-activity studies from activity-dependent neuroprotective protein, activity-dependent neuroprotective protein (ADNP). ADNP is essential for brain formation. ADNP haploinsufficiency in mice results in tauopathy and cognitive deficits ameliorated by davunetide treatment. This article summarizes in brief recent reviews about NAP protection against tauopathy including the all D-amino acid analogue-D-NAP (AL-408). D-NAP was discovered to have similar neuroprotective functions to NAP in vitro. Here, D-NAP was tested as prophylactic as well as therapeutic treatment for amytrophic lateral sclerosis (ALS) in the widely used TgN(SOD1-G93A)1Gur transgenic mouse model. Results showed D-NAP-associated prophylactic protection, thus daily treatment starting from day 2 of age resulted in a prolonged life course in the D-NAP-treated mice, which was coupled to a significant decrease in tau hyperphosphorylation. These studies correlate protection against tau hyperphosphorylation and longevity in a severe model of ALS-like motor impairment and early mortality. NAP is a first-in-class drug candidate/investigation compound providing neuroprotection coupled to inhibition of tau pathology. D-NAP (AL-408) is a pipeline product.

  1. Sex and experience in the academy award nomination process.

    PubMed

    Lincoln, Anne E

    2004-10-01

    Research has indicated significant age differences between male and female Academy Award nominees and winners. However, this discrepancy may be associated with sex differences in actors' ages when they first begin their acting careers. The present research uses event history analysis to investigate the duration of Academy Award nominees' careers from career start (first film) to first three Academy Award nominations. Analysis suggested controlling for an actor's age at first film explains the sex-age disparity between Academy Award nominees and winners.

  2. Efficacy of Napping Strategies to Counter the Effects of Sleep Deprivation

    DTIC Science & Technology

    2002-11-01

    Parrino , Cesana, and Priore. 1990; napping conditions (Bonnet, 1990; Carskadon and Matsumoto and Harada, 1994; Rogers, Spencer, Stone, Dement, 1982...consistent with other reports (Lorizio, Terzano, VAS Parrino , Cesana, and Priore, 1990; Sanger, Perrault, The VAS scores were analyzed in an ANOVA Morel, Joly...Wesensten, N., Lorizio, A., Terzano, M., Parrino ,L., Cesana, B., and McCann, U., and Balance, G. 1992. Comparison of Priore, P. 1990. Zolpidem: a

  3. Fully automatic guidance for rotorcraft nap-of-the-earth (NOE) flight following planned profiles

    NASA Technical Reports Server (NTRS)

    Gorder, Peter J.; Clement, Warren F.; Jewell, Wayne F.

    1989-01-01

    Developing a single-pilot, all-weather nap-of-the-earth (NOE) capability requires fully automatic NOE navigation and flight control. Innovative guidance and control concepts are being investigated in a fourfold research effort that will culminate in the real-time piloted simulation of promising alternatives for automatic guidance and control of rotorcraft in NOE operations, thereby providing a practical demonstration for evaluating pilot acceptance of the automated concepts.

  4. The Effect of a Daytime Nap on Priming and Recognition Tasks in Preschool Children

    PubMed Central

    Giganti, Fiorenza; Arzilli, Cinzia; Conte, Francesca; Toselli, Monica; Viggiano, Maria Pia; Ficca, Gianluca

    2014-01-01

    Study Objectives: The beneficial effect of sleep on memory consolidation is widely accepted in the adult population and has recently been shown in children. However, the few available data almost exclusively refer to school-aged children. Here we explore the effect of a daytime nap on memory consolidation in a sample of preschool children. Design: Subjects performed both a figures recognition task and a priming task, in order to differentiate effects on explicit and implicit memory. Setting: Nursery school. Participants: Twenty-three children (mean age: 52.6 ± 8 mo; 13 males) participated in the study. Intervention: After a study phase in which children had to name 40 pictures of objects and animals, each subject either took an actigraphically monitored nap or stayed awake. At retest, children were administered both an implicit and an explicit memory task. Measurements and Results: The implicit memory task consisted of naming 40 pictures presented at eight ascending levels of spatial filtering. The explicit memory task consisted of judging 40 pictures as old or new. The number of correct answers at the explicit recognition task was significantly higher in the nap compared to the wake condition, whereas priming effects did not differ between conditions. Conclusions: A positive role of sleep in explicit memory consolidation, similar to the one observed in the adult, was detected in our sample of preschool children. In contrast, our data suggest that implicit perceptual learning, involved in priming tasks, does not benefit from sleep. Citation: Giganti F, Arzilli C, Conte F, Toselli M, Viggiano MP, Ficca G. The effect of a daytime nap on priming and recognition tasks in preschool children. SLEEP 2014;37(6):1087-1093. PMID:24882903

  5. Erotic subset for the Nencki Affective Picture System (NAPS ERO): cross-sexual comparison study

    PubMed Central

    Wierzba, Małgorzata; Riegel, Monika; Pucz, Anna; Leśniewska, Zuzanna; Dragan, Wojciech Ł.; Gola, Mateusz; Jednoróg, Katarzyna; Marchewka, Artur

    2015-01-01

    Research on the processing of sexual stimuli has proved that such material has high priority in human cognition. Yet, although sex differences in response to sexual stimuli were extensively discussed in the literature, sexual orientation was given relatively little consideration, and material suitable for relevant research is difficult to come by. With this in mind, we present a collection of 200 erotic images, accompanied by their self-report ratings of emotional valence and arousal by homo- and heterosexual males and females (n = 80, divided into four equal-sized subsamples). The collection complements the Nencki Affective Picture System (NAPS) and is intended to be used as stimulus material in experimental research. The erotic images are divided into five categories, depending on their content: opposite-sex couple (50), male couple (50), female couple (50), male (25) and female (25). Additional 100 control images from the NAPS depicting people in a non-erotic context were also used in the study. We showed that recipient sex and sexual orientation strongly influenced the evaluation of erotic content. Thus, comparisons of valence and arousal ratings in different subject groups will help researchers select stimuli set for the purpose of various experimental designs. To facilitate the use of the dataset, we provide an on-line tool, which allows the user to browse the images interactively and select proper stimuli on the basis of several parameters. The NAPS ERO image collection together with the data are available to the scientific community for non-commercial use at http://naps.nencki.gov.pl. PMID:26441715

  6. Removal of Ca2+ and Zn2+ from aqueous solutions by zeolites NaP and KP.

    PubMed

    Yusof, Alias Mohd; Malek, Nik Ahmad Nizam Nik; Kamaruzaman, Nurul Asyikin; Adil, Muhammad

    2010-01-01

    Zeolites P in sodium (NaP) and potassium (KP) forms were used as adsorbents for the removal of calcium (Ca2+) and zinc (Zn2+) cations from aqueous solutions. Zeolite KP was prepared by ion exchange of K+ with Na+ which neutralizes the negative charge of the zeolite P framework structure. The ion exchange capacity of K+ on zeolite NaP was determined through the Freundlich isotherm equilibrium study. Characterization of zeolite KP was determined using infrared spectroscopy and X-ray diffraction (XRD) techniques. From the characterization, the structure of zeolite KP was found to remain stable after the ion exchange process. Zeolites KP and NaP were used for the removal of Ca and Zn from solution. The amount of Ca2+ and Zn2+ in aqueous solution before and after the adsorption by zeolites was analysed using the flame atomic absorption spectroscopy method. The removal of Ca2+ and Zn2+ followed the Freundlich isotherm rather than the Langmuir isotherm model. This result also revealed that zeolite KP adsorbs Ca2+ and Zn2+ more than zeolite NaP and proved that modification of zeolite NaP with potassium leads to an increase in the adsorption efficiency of the zeolite. Therefore, the zeolites NaP and KP can be used for water softening (Ca removal) and reducing water pollution/toxicity (Zn removal).

  7. Effects of napping on sleepiness and sleep-related performance deficits in night-shift workers: a systematic review.

    PubMed

    Ruggiero, Jeanne S; Redeker, Nancy S

    2014-04-01

    Night-shift workers are prone to sleep deprivation, misalignment of circadian rhythms, and subsequent sleepiness and sleep-related performance deficits. The purpose of this narrative systematic review is to critically review and synthesize the scientific literature regarding improvements in sleepiness and sleep-related performance deficits following planned naps taken during work-shift hours by night workers and to recommend directions for future research and practice. We conducted a literature search using the Medline, PsychInfo, CINAHL, Cochrane Library, and Health and Safety Science Abstracts databases and included English-language quasi-experimental and experimental studies that evaluated the effects of a nighttime nap taken during a simulated or actual night-work shift. We identified 13 relevant studies, which consisted primarily of small samples and mixed designs. Most investigators found that, despite short periods of sleep inertia immediately following naps, night-shift napping led to decreased sleepiness and improved sleep-related performance. None of the studies examined the effects of naps on safety outcomes in the workplace. Larger-scale randomized clinical trials of night-shift napping and direct safety outcomes are needed prior to wider implementation.

  8. NapA protects Helicobacter pylori from oxidative stress damage, and its production is influenced by the ferric uptake regulator.

    PubMed

    Cooksley, Clare; Jenks, Peter J; Green, Andrew; Cockayne, Alan; Logan, Robert P H; Hardie, Kim R

    2003-06-01

    The Helicobacter pylori protein NapA has been identified as a homologue of the Escherichia coli protein Dps. It is shown in this study that, like Dps, NapA is produced maximally in stationary phase cells and contributes to the ability of H. pylori to survive under oxidative stress conditions. Moreover, NapA co-localizes with the nuclear material, suggesting that it can interact with DNA in vivo. Furthermore, it is demonstrated that repression of NapA production by iron starvation was not so pronounced in a H. pylori fur mutant, suggesting that the ferric uptake regulator (Fur) is involved in napA regulation, and a potential fur box by which this control could be mediated is identified. This finding is consistent with the regulation of iron-binding proteins by Fur and also the modulation of Fur during oxidative stress, thus allowing NapA levels to be increased in the environmental conditions under which its ability to protect DNA from attack by toxic free radicals is most beneficial to the cell.

  9. Activity-dependent neuroprotective protein (ADNP)-derived peptide (NAP) ameliorates hypobaric hypoxia induced oxidative stress in rat brain.

    PubMed

    Sharma, Narendra K; Sethy, Niroj K; Meena, Ram Niwas; Ilavazhagan, Govindsamy; Das, Mainak; Bhargava, Kalpana

    2011-06-01

    Hypobaric hypoxia is a socio-economic problem affecting cognitive, memory and behavior functions. Severe oxidative stress caused by hypobaric hypoxia adversely affects brain areas like cortex, hippocampus, basal ganglia, and cerebellum. In the present study, we have investigated the antioxidant and memory protection efficacy of the synthetic NAP peptide (NAPVSIPQ) during long-term chronic hypobaric hypoxia (7, 14, 21 and 28 days, 25,000ft) in rats. Intranasal supplementation of NAP peptide (2μg/Kg body weight) improved antioxidant status of brain evaluated by biochemical assays for free radical estimation, lipid peroxidation, GSH and GSSG level. Analysis of expression levels of SOD revealed that NAP significantly activated antioxidant genes as compared to hypoxia exposed rats. We have also observed a significant increased expression of Nrf2, the master regulator of antioxidant defense system and its downstream targets such as HO-1, GST and SOD1 by NAP supplementation, suggesting activation of Nrf2-mediated antioxidant defense response. In corroboration, our results also demonstrate that NAP supplementation improved the memory function assessed with radial arm maze. These cumulative results suggest the therapeutic potential of NAP peptide for ameliorating hypobaric hypoxia-induced oxidative stress.

  10. HP-NAP inhibits the growth of bladder cancer in mice by activating a cytotoxic Th1 response.

    PubMed

    Codolo, Gaia; Fassan, Matteo; Munari, Fabio; Volpe, Andrea; Bassi, Piefrancesco; Rugge, Massimo; Pagano, Francesco; D'Elios, Mario Milco; de Bernard, Marina

    2012-01-01

    Intravesical Bacillus Calmette-Guérin (BCG) is the gold standard treatment for intermediate and high-risk non-muscle-invasive bladder cancer. BCG therapy is the most successful example of immunotherapy in cancer. Unfortunately, the treatment-related side effects are still relevant. Furthermore, non-responder patients are candidate to radical cystectomy in the absence of valuable alternative options. These aspects have prompted the search for newer biological response modifiers (BRM) with a better benefit/side effects ratio. The toll-like receptor (TLR) 2 ligand, Helicobacter pylori protein HP-NAP, has been shown to deserve a potential role as BRM. HP-NAP is capable of driving the differentiation of T helper (Th) 1 cells, both in vitro and in vivo, because of its ability to create an IL-12-enriched milieu. Herein, we report that local administration of HP-NAP decreases tumour growth by triggering tumour necrosis in a mouse model of bladder cancer implant. The effect is accompanied by a significant accumulation of both CD4+ and CD8+ IFN-γ-secreting cells, within tumour and regional lymph nodes. Noteworthy, HP-NAP-treated tumours show also a reduced vascularization due to the anti-angiogenic activity of IFN-γ induced by HP-NAP. Our findings strongly indicate that HP-NAP might become a novel therapeutic "bullet" for the cure of bladder tumours.

  11. The impact of napping on memory for future-relevant stimuli: Prioritization among multiple salience cues.

    PubMed

    Bennion, Kelly A; Payne, Jessica D; Kensinger, Elizabeth A

    2016-06-01

    Prior research has demonstrated that sleep enhances memory for future-relevant information, including memory for information that is salient due to emotion, reward, or knowledge of a later memory test. Although sleep has been shown to prioritize information with any of these characteristics, the present study investigates the novel question of how sleep prioritizes information when multiple salience cues exist. Participants encoded scenes that were future-relevant based on emotion (emotional vs. neutral), reward (rewarded vs. unrewarded), and instructed learning (intentionally vs. incidentally encoded), preceding a delay consisting of a nap, an equivalent time period spent awake, or a nap followed by wakefulness (to control for effects of interference). Recognition testing revealed that when multiple dimensions of future relevance co-occur, sleep prioritizes top-down, goal-directed cues (instructed learning, and to a lesser degree, reward) over bottom-up, stimulus-driven characteristics (emotion). Further, results showed that these factors interact; the effect of a nap on intentionally encoded information was especially strong for neutral (relative to emotional) information, suggesting that once one cue for future relevance is present, there are diminishing returns with additional cues. Sleep may binarize information based on whether it is future-relevant or not, preferentially consolidating memory for the former category. Potential neural mechanisms underlying these selective effects and the implications of this research for educational and vocational domains are discussed. (PsycINFO Database Record

  12. Protocadherin-18b interacts with Nap1 to control motor axon growth and arborization in zebrafish

    PubMed Central

    Biswas, Sayantanee; Emond, Michelle R.; Duy, Phan Q.; Hao, Le T.; Beattie, Christine E.; Jontes, James D.

    2014-01-01

    The proper assembly of neural circuits during development requires the precise control of axon outgrowth, guidance, and arborization. Although the protocadherin family of cell surface receptors is widely hypothesized to participate in neural circuit assembly, their specific roles in neuronal development remain largely unknown. Here we demonstrate that zebrafish pcdh18b is involved in regulating axon arborization in primary motoneurons. Although axon outgrowth and elongation appear normal, antisense morpholino knockdown of pcdh18b results in dose-dependent axon branching defects in caudal primary motoneurons. Cell transplantation experiments show that this effect is cell autonomous. Pcdh18b interacts with Nap1, a core component of the WAVE complex, through its intracellular domain, suggesting a role in the control of actin assembly. Like that of Pcdh18b, depletion of Nap1 results in reduced branching of motor axons. Time-lapse imaging and quantitative analysis of axon dynamics indicate that both Pcdh18b and Nap1 regulate axon arborization by affecting the density of filopodia along the shaft of the extending axon. PMID:24371087

  13. Silencing of the SlNAP7 gene influences plastid development and lycopene accumulation in tomato

    NASA Astrophysics Data System (ADS)

    Fu, Da-Qi; Meng, Lan-Huan; Zhu, Ben-Zhong; Zhu, Hong-Liang; Yan, Hua-Xue; Luo, Yun-Bo

    2016-12-01

    Ripening is an important stage of fruit development. To screen the genes associated with pigment formation in tomato fruit, a suppression subtractive hybridization (SSH) cDNA library was constructed by using tomato fruit in the green ripe and break ripe stages, and 129 differential genes were obtained. Using redness as a screening marker, virus-induced gene silencing (VIGS) of the differential genes was performed with a sprout vacuum-infiltration system (SVI). The results showed that silencing the SlNAP7 gene affected the chloroplast development of tomato leaves, manifesting as a photo-bleaching phenotype, and silenced fruit significantly affected the accumulation of lycopene, manifested as a yellow phenotype. In our study, we found that silencing the SlNAP7 gene downregulates the expression of the POR and PORA genes and destroys the normal development of the chloroplast. The expression of related genes included in the lycopene biosynthesis pathway was not significantly changed, but lycopene accumulation was significantly reduced in tomato fruit. Perhaps it was caused by the destruction of the chromoplast, which leads to the oxidation of lycopene. The results show that the SlNAP7 gene influences chloroplast development and lycopene accumulation in tomato.

  14. Silencing of the SlNAP7 gene influences plastid development and lycopene accumulation in tomato.

    PubMed

    Fu, Da-Qi; Meng, Lan-Huan; Zhu, Ben-Zhong; Zhu, Hong-Liang; Yan, Hua-Xue; Luo, Yun-Bo

    2016-12-08

    Ripening is an important stage of fruit development. To screen the genes associated with pigment formation in tomato fruit, a suppression subtractive hybridization (SSH) cDNA library was constructed by using tomato fruit in the green ripe and break ripe stages, and 129 differential genes were obtained. Using redness as a screening marker, virus-induced gene silencing (VIGS) of the differential genes was performed with a sprout vacuum-infiltration system (SVI). The results showed that silencing the SlNAP7 gene affected the chloroplast development of tomato leaves, manifesting as a photo-bleaching phenotype, and silenced fruit significantly affected the accumulation of lycopene, manifested as a yellow phenotype. In our study, we found that silencing the SlNAP7 gene downregulates the expression of the POR and PORA genes and destroys the normal development of the chloroplast. The expression of related genes included in the lycopene biosynthesis pathway was not significantly changed, but lycopene accumulation was significantly reduced in tomato fruit. Perhaps it was caused by the destruction of the chromoplast, which leads to the oxidation of lycopene. The results show that the SlNAP7 gene influences chloroplast development and lycopene accumulation in tomato.

  15. Redox metabolites signal polymicrobial biofilm development via the NapA oxidative stress cascade in Aspergillus

    PubMed Central

    Zheng, He; Kim, Jaekuk; Liew, Mathew; Yan, John K.; Herrera, Oscar; Bok, JinWoo; Kelleher, Neil L.; Keller, Nancy P.; Wang, Yun

    2014-01-01

    Summary Background Filamentous fungi and bacteria form mixed-species biofilms in nature and diverse clinical contexts. They secrete a wealth of redox-active small molecule secondary metabolites, which are traditionally viewed as toxins that inhibit growth of competing microbes. Results Here we report that these “toxins” can act as interspecies signals, affecting filamentous fungal development via oxidative stress regulation. Specifically, in co-culture biofilms, Pseudomonas aeruginosa phenazine-derived metabolites differentially modulated Aspergillus fumigatus development, shifting from weak vegetative growth to induced asexual sporulation (conidiation) along a decreasing phenazine gradient. The A. fumigatus morphological shift correlated with the production of phenazine radicals and concomitant reactive oxygen species (ROS) production generated by phenazine redox cycling. Phenazine conidiation signaling was conserved in the genetic model A. nidulans, and mediated by NapA, a homolog of AP-1-like bZIP transcription factor, which is essential for the response to oxidative stress in humans, yeast, and filamentous fungi. Expression profiling showed phenazine treatment induced a NapA-dependent response of the global oxidative stress metabolome including the thioredoxin, glutathione and NADPH-oxidase systems. Conidiation induction in A. nidulans by another microbial redox-active secondary metabolite, gliotoxin, also required NapA. Conclusions This work highlights that microbial redox metabolites are key signals for sporulation in filamentous fungi, which are communicated through an evolutionarily conserved eukaryotic stress response pathway. It provides a foundation for interspecies signaling in environmental and clinical biofilms involving bacteria and filamentous fungi. PMID:25532893

  16. The differential effects of emotional salience on direct associative and relational memory during a nap.

    PubMed

    Alger, Sara E; Payne, Jessica D

    2016-12-01

    Relational memories are formed from shared components between directly learned memory associations, flexibly linking learned information to better inform future judgments. Sleep has been found to facilitate both direct associative and relational memories. However, the impact of incorporating emotionally salient information into learned material and the interaction of emotional salience and sleep in facilitating both types of memory is unknown. Participants encoded two sets of picture pairs, with either emotionally negative or neutral objects paired with neutral faces. The same objects were present in both sets, paired with two different faces across the sets. Baseline memory for these directly paired associates was tested immediately after encoding, followed by either a 90-min nap opportunity or wakefulness. Five hours after learning, a surprise test assessed relational memory, the indirect association between two faces paired with the same object during encoding, followed by a retest of direct associative memory. Overall, negative information was remembered better than neutral for directly learned pairs. A nap facilitated both preservation of direct associative memories and formation of relational memories, compared to remaining awake. Interestingly, however, this sleep benefit was observed specifically for neutral directly paired associates, while both neutral and negative relational associations benefitted from a nap. Finally, REM sleep played opposing roles in neutral direct and relational associative memory formation, with more REM sleep leading to forgetting of direct associations but promoting relational associations, suggesting that, while not benefitting memory consolidation for directly learned details, REM sleep may foster the memory reorganization needed for relational memory.

  17. Procedural performance following sleep deprivation remains impaired despite extended practice and an afternoon nap

    PubMed Central

    Kurniawan, Irma Triasih; Cousins, James Nicholas; Chong, Pearlynne L. H.; Chee, Michael W. L.

    2016-01-01

    The negative impact of sleep loss on procedural memory is well established, yet it remains unclear how extended practice opportunities or daytime naps can modulate the effect of a night of sleep deprivation. Here, participants underwent three training and test conditions on a sequential finger tapping task (SFTT) separated by at least one week. In the first condition they were trained in the evening followed by a night of sleep. Two further conditions took place where evening training was followed by a night of total sleep deprivation (TSD). One of the TSD conditions included a one-hour nap opportunity (15:00). Compared to the condition in which sleep was permitted, a night of TSD resulted in poorer performance across 4 practices the following day (10:00–19:00). The deleterious effect of a single night of TSD on procedural performance, was neither clearly alleviated by an afternoon nap nor by multiple practice opportunities. Interestingly, significant gains in performance were observed in all conditions after a one-week delay. Recovery sleep on subsequent nights thus appeared to nullify the effect of a single night of sleep deprivation, underscoring the importance of offline consolidation on the acquisition of procedural skill. PMID:27782172

  18. Recovery of subsampled dimensions and configurations derived from napping data by MFA and MDS.

    PubMed

    Nestrud, Michael A; Lawless, Harry T

    2011-05-01

    Napping is a multivariate sensory method in which participants physically place stimuli on a large sheet of paper and orient them so that the distance between pairs represents a measure of dissimilarity. The two-dimensional nature of the task may be a limitation to the ability of this and similar methodologies to recover information about complex stimuli. In the first investigation, eight simulated three-dimensional stimuli were created with two different levels for each attribute. Simulated napping experiments had groups of participants attend to two of the dimensions with different probabilities. Multiple factor analysis (an analytical multivariate statistical procedure that can be thought of as a principle components analysis on the individuals) and MDS-INDSCAL (a variation on multidimensional scaling that finds a common configuration through reducing a stress measure associated with lack of fit) recovered full dimensionality from these data, although MFA had trouble when attention was the most unbalanced. In the second experiment, a human napping experiment was designed using custom three-dimensional stimuli: shapes with two levels each of size, color, and shape attributes. This experiment confirmed the results of Experiment 1, as both MDS-INDSCAL and MFA analyses again recovered the full dimensionality of the stimuli.

  19. Silencing of the SlNAP7 gene influences plastid development and lycopene accumulation in tomato

    PubMed Central

    Fu, Da-Qi; Meng, Lan-Huan; Zhu, Ben-Zhong; Zhu, Hong-Liang; Yan, Hua-Xue; Luo, Yun-Bo

    2016-01-01

    Ripening is an important stage of fruit development. To screen the genes associated with pigment formation in tomato fruit, a suppression subtractive hybridization (SSH) cDNA library was constructed by using tomato fruit in the green ripe and break ripe stages, and 129 differential genes were obtained. Using redness as a screening marker, virus-induced gene silencing (VIGS) of the differential genes was performed with a sprout vacuum-infiltration system (SVI). The results showed that silencing the SlNAP7 gene affected the chloroplast development of tomato leaves, manifesting as a photo-bleaching phenotype, and silenced fruit significantly affected the accumulation of lycopene, manifested as a yellow phenotype. In our study, we found that silencing the SlNAP7 gene downregulates the expression of the POR and PORA genes and destroys the normal development of the chloroplast. The expression of related genes included in the lycopene biosynthesis pathway was not significantly changed, but lycopene accumulation was significantly reduced in tomato fruit. Perhaps it was caused by the destruction of the chromoplast, which leads to the oxidation of lycopene. The results show that the SlNAP7 gene influences chloroplast development and lycopene accumulation in tomato. PMID:27929131

  20. Haberdashers' Aske's: The Campaign against Academies in Lewisham

    ERIC Educational Resources Information Center

    Powell-Davies, Martin

    2008-01-01

    The National Union of Teachers' 2007 pamphlet "Academies--Looking Beyond the Spin: why the NUT calls for a different approach" lists six reasons why the NUT opposes Academies, schools run by private sponsors using public money. In this article, the Secretary of the Lewisham NUT presents evidence to show how the Academies operated by the…

  1. The Racial History of the U.S. Military Academies.

    ERIC Educational Resources Information Center

    Hansen, Susan D.

    1999-01-01

    Presents the history of the entry of African American students and faculty into the nation's prestigious military institutions: the U.S. Military Academy at West Point, the U.S. Naval Academy at Annapolis, and the U.S. Air Force Academy in Colorado Springs. Notes that the military's effort to diversify its troops and officer corps is a recent…

  2. The U.S. Coast Guard Academy Curricula; An Evaluation.

    ERIC Educational Resources Information Center

    Powers, Theodore R.; And Others

    This report presents the results of research that had as its objective the evaluation of the curricula of the U.S. Coast Guard Academy. Over 125 Coast Guard officers and men were interviewed to gather detailed background information about the Coast Guard, the Coast Guard Academy, and job requirements of Academy graduates. These data were developed…

  3. Day of Dialogue: Research Priorities from the Researching Academies Conference

    ERIC Educational Resources Information Center

    Woods, Glenys J.; Woods, Philip A.

    2009-01-01

    The Researching Academies (RA) Conference, hosted by the University of Gloucestershire in Cheltenham on 12 November 2008 and organised by the authors, was a unique one-day event, funded as part of a research project (a case study of an Academy) supported by a British Academy (BA) grant, with additional support and funding from BELMAS. The RA…

  4. 75 FR 39561 - National Fire Academy Board of Visitors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-09

    ... SECURITY Federal Emergency Management Agency National Fire Academy Board of Visitors AGENCY: Federal... Meeting. SUMMARY: The National Fire Academy Board of Visitors will meet by teleconference on August 2... or comments received by the National Fire Academy Board of Visitors, go to...

  5. 76 FR 17425 - National Fire Academy Board of Visitors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-29

    ... SECURITY Federal Emergency Management Agency National Fire Academy Board of Visitors AGENCY: Federal... Meeting. SUMMARY: The National Fire Academy Board of Visitors will meet on April 6-7, 2011. The meeting... the National Fire Academy Board of Visitors, go to http://www.regulations.gov . A public...

  6. 75 FR 2153 - National Fire Academy Board of Visitors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-14

    ... SECURITY Federal Emergency Management Agency National Fire Academy Board of Visitors AGENCY: Federal... Advisory Committee Meeting. SUMMARY: The National Fire Academy Board of Visitors will meet by... documents or comments received by the National Fire Academy Board of Visitors, go to...

  7. 76 FR 20696 - National Fire Academy Board of Visitors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-13

    ... SECURITY Federal Emergency Management Agency National Fire Academy Board of Visitors AGENCY: Federal... Federal Register at 76 FR 17425 that the National Fire Academy Board of Visitors would meet on April 6 and... National Fire Academy Board of Visitors meeting was held on Wednesday, April 6, 2011, from 8:30 a.m. to 5...

  8. 75 FR 18524 - National Fire Academy Board of Visitors

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-12

    ... SECURITY Federal Emergency Management Agency National Fire Academy Board of Visitors AGENCY: Federal... Meeting. ] SUMMARY: The National Fire Academy Board of Visitors will meet on April 28-29, 2010. DATES: The... background documents or comments received by the National Fire Academy Board of Visitors, go to...

  9. Law and the Student Press.

    ERIC Educational Resources Information Center

    Stevens, George E.; Webster, John B.

    Court cases and legal decisions involving the student press in the late 1960s and early 1970s are brought together in this book in order to show how the law has been applied to school officials and student journalists in high school, college, and the underground. The ten chapters cover the following topics: censorship, libel, obscenity, contempt,…

  10. Gifted Education Press Quarterly, 1995.

    ERIC Educational Resources Information Center

    Fisher, Maurice D., Ed.

    1995-01-01

    This document consists of the four issues of the newsletter "Gifted Education Press Quarterly" published during 1995. This newsletter addresses issues in the education of gifted children and youth. The major articles are: (1) "Using Today's Technology: Parents Can Help Challenge Gifted Children" (Adrienne O'Neill); (2)…

  11. The Alternative Press in Microform.

    ERIC Educational Resources Information Center

    Tsang, Daniel C.

    Presented is an analysis of what has been done regarding the preservation of alternative publications in microfilm. The alternative press was defined, for the purposes of this study, as "nonstandard, nonestablishment publications." Two projects undertaken to microfilm such publications, one by the Microphoto Division of Bell and Howell…

  12. The Schools and the Press.

    ERIC Educational Resources Information Center

    Lewis, Ann Chambers

    This book acquaints the school administrator with the need for good press relations and suggests ways to obtain such relations. A brief overview of the internal workings and concerns of a newspaper staff is presented. Methods of writing news copy for release and how to time releases for maximum news coverage are explained. Throughout the volume,…

  13. Censorship and the Student Press.

    ERIC Educational Resources Information Center

    Oettinger, Lauren

    The First Amendment rights provided to journalists--freedom of speech and of the press--are fundamental beliefs held by Americans. What students learn in the government class, however, may not be what they learn as editors-in-chief of student newspapers. U.S. schools are being charged with the censorship of student publications at an…

  14. Mars Observer Press Conference JPL

    NASA Astrophysics Data System (ADS)

    1993-08-01

    The Mars Observer mission spacecraft was primarily designed for exploring Mars and the Martian environment. The Mars Observer was launched on September 25, 1992. The spacecraft was lost in the vicinity of Mars on August 21, 1993 when the spacecraft began its maneuvering sequence for Martian orbital insertion. This videotape shows a press briefing, held after the spacecraft had not responded to attempts to communicate with it, to explain to the press the problems and the steps that were being taken to re-establish communication with the spacecraft. The communications had been shutdown prior to the orbital insertion burn to protect the instruments. At the time of the press conference, the communications system was still not operational, and attempts were being made to re-establish communication. Bob McMillan of the Public Affairs Office at JPL gives the initial announcement of the continuing communication problem with the spacecraft. Mr. McMillan introduces William Piotrowski, acting director of solar system exploration, who reiterates that there is indeed no communication with the Observer spacecraft. He is followed by Glenn Cunningham, the Project Manager of the Mars Observer who speaks about the attempts to re-establish contact. Mr. Cunningham is followed by Satenios Dallas, the Mission Manager for the Mars Observer Project, who speaks about the sequence of events leading up to the communication failure, and shows an animated video presenting the orbital insertion maneuvers. The briefing was then opened up for questions from the assembled press, both at JPL and at the other NASA Centers. The questions are about the possible reasons for the communication failure, and the attempts to restore communications with the spacecraft. Dr. Arden L. Albee, chief scientist for the Mars Observer Mission, joins the other panel members to answer questions. At the end of the press briefing the animation of the Mars orbital insertion is shown again.

  15. Mars Observer Press Conference JPL

    NASA Technical Reports Server (NTRS)

    1993-01-01

    The Mars Observer mission spacecraft was primarily designed for exploring Mars and the Martian environment. The Mars Observer was launched on September 25, 1992. The spacecraft was lost in the vicinity of Mars on August 21, 1993 when the spacecraft began its maneuvering sequence for Martian orbital insertion. This videotape shows a press briefing, held after the spacecraft had not responded to attempts to communicate with it, to explain to the press the problems and the steps that were being taken to re-establish communication with the spacecraft. The communications had been shutdown prior to the orbital insertion burn to protect the instruments. At the time of the press conference, the communications system was still not operational, and attempts were being made to re-establish communication. Bob McMillan of the Public Affairs Office at JPL gives the initial announcement of the continuing communication problem with the spacecraft. Mr. McMillan introduces William Piotrowski, acting director of solar system exploration, who reiterates that there is indeed no communication with the Observer spacecraft. He is followed by Glenn Cunningham, the Project Manager of the Mars Observer who speaks about the attempts to re-establish contact. Mr. Cunningham is followed by Satenios Dallas, the Mission Manager for the Mars Observer Project, who speaks about the sequence of events leading up to the communication failure, and shows an animated video presenting the orbital insertion maneuvers. The briefing was then opened up for questions from the assembled press, both at JPL and at the other NASA Centers. The questions are about the possible reasons for the communication failure, and the attempts to restore communications with the spacecraft. Dr. Arden L. Albee, chief scientist for the Mars Observer Mission, joins the other panel members to answer questions. At the end of the press briefing the animation of the Mars orbital insertion is shown again.

  16. Predominance and high antibiotic resistance of the emerging Clostridium difficile genotypes NAPCR1 and NAP9 in a Costa Rican hospital over a 2-year period without outbreaks

    PubMed Central

    López-Ureña, Diana; Quesada-Gómez, Carlos; Montoya-Ramírez, Mónica; del Mar Gamboa-Coronado, María; Somogyi, Teresita; Rodríguez, César; Rodríguez-Cavallini, Evelyn

    2016-01-01

    Clostridium difficile is the major causative agent of nosocomial antibiotic-associated diarrhea. In a 2009 outbreak of C. difficile-associated diarrhea that was recorded in a major Costa Rican hospital, the hypervirulent NAP1 strain (45%) predominated together with a local genotype variant (NAPCR1, 31%). Both strains were fluoroquinolone-resistant and the NAPCR1 genotype, in addition, was resistant to clindamycin and rifampicin. We now report on the genotypes and antibiotic susceptibilities of 68 C. difficile isolates from a major Costa Rican hospital over a 2-year period without outbreaks. In contrast to our previous findings, no NAP1 strains were detected, and for the first time in a Costa Rican hospital, a significant fraction of the isolates were NAP9 strains (n=14, 21%). The local NAPCR1 genotype remained prevalent (n=18, 26%) and coexisted with 14 strains (21%) of classic hospital NAP types (NAP2, NAP4, and NAP6), eight new genotypes (12%), four environmental strains classified as NAP10 or NAP11 (6%), three strains without NAP designation (4%) and seven non-toxigenic strains (10%). All 68 strains were resistant to ciprofloxacin, 88% were resistant to clindamycin and 50% were resistant to moxifloxacin and rifampicin. Metronidazole and vancomycin susceptibilities were universal. The NAPCR1 and NAP9 strains, which have been associated with more severe clinical infections, were more resistant to antibiotics than the other strains. Altogether, our results confirm that the epidemiology of C. difficile infection is dynamic and that A−B+ strains from the NAP9 type are on the rise not only in the developed world. Moreover, our results reveal that the local NAPCR1 strains still circulate in the country without causing outbreaks but with equally high antibiotic-resistance rates and levels. PMID:27165560

  17. [The Military Medical Academy during the Great Patriotic War].

    PubMed

    Elizarev, S V; Lemeshkin, R N; Klyuchnkov, P B

    2015-05-01

    The article is devoted to the 70th anniversary of Victory in the Great Patriotic War of 1941-1945. Presented the contribution of the"Military Medical Academy. Examined the activities of the academy and the fulfillment of its employees on the eve of the war, the conditions under which employees had to work in the first months of the war in Leningrad. Described the evacuation of the Academy to the rear of the' country and especially educational, scientific and practical activities of employees Academy in Samarkand; activities clinics Academy remaining in the besieged Leningrad; radical reorganization of the Academy during the war. Presented briefly the history of creation of command and the Medical Faculty. Paying tribute to the faculty of the Academy in the field of research, teaching activities and practical work in the army.

  18. Hanford Patrol Academy demolition sites closure plan

    SciTech Connect

    Not Available

    1993-09-30

    The Hanford Site is owned by the U.S. Government and operated by the U.S. Department of Energy, Richland Operations Office. Westinghouse Hanford Company is a major contractor to the U.S. Department of Energy, Richland Operations Office and serves as co-operator of the Hanford Patrol Academy Demolition Sites, the unit addressed in this paper. This document consists of a Hanford Facility Dangerous Waste Part A Permit Application, Form 3 (Revision 4), and a closure plan for the site. An explanation of the Part A Form 3 submitted with this closure plan is provided at the beginning of the Part A section. This Hanford Patrol Academy Demolition Sites Closure Plan submittal contains information current as of December 15, 1994.

  19. 2010 Service Academy Gender Relations Survey

    DTIC Science & Technology

    2010-12-15

    assault. They provided feedback on the actions of Academy senior leadership, officers and non-commissioned officers (NCOs)/chief petty officers (CPOs) in... Efl ^E^H 23 Senior | Junior | ^^^^^•Z^^l Sophomore KEfl MJM 19 Freshman...confidential, and no follow-up action will be taken in response to any specifics reported. Your feedback is useful and appreciated. Please print. II J

  20. 2011 Service Academy Gender Relations Focus Group

    DTIC Science & Technology

    2011-09-01

    you are the experts on Academy life and as the last topic for today I want to see what your recommendations are for improvements . Time for...and victim care procedures. Recurring evaluation of these programs is helpful for developing process improvements aimed at reducing instances of...by U.S. Code 10. However, USCGA officials requested they be included, beginning in 2008, in order to evaluate and improve their programs addressing

  1. 2008 Service Academy Gender Relations Survey

    DTIC Science & Technology

    2008-12-01

    experienced stalking- related behaviors and the incident caused them fear of physical harm or sexual assault (the requirement to meet the legal definition of...incident caused them fear of physical harm or sexual assault (the requirement to meet the legal definition of stalking). U.S. Air Force Academy Unwanted...incident caused them fear of physical harm or sexual assault (the requirement to meet the legal definition of stalking). Table of Contents xi Page

  2. Antitumor and immunomodulatory effects of recombinant fusion protein rMBP-NAP through TLR-2 dependent mechanism in tumor bearing mice.

    PubMed

    Wang, Ting; Liu, Xilong; Ji, Zhenyu; Men, Yingli; Du, Mingxuan; Ding, Cong; Wu, Yahong; Liu, Xin; Kang, Qiaozhen

    2015-12-01

    The pro-inflammatory and immunomodulatory properties of Helicobacter pylori neutrophil activating protein (Hp-NAP) not only make it to play an important role in disease pathogenesis but also make it to be a potential candidate for therapeutic applications, including vaccine and drug development. Our previous work demonstrated that the recombinant Hp-NAP fused with the maltose binding protein of Escherichia coli (rMBP-NAP) play an important role in regulating the differentiation of Th1 cells. In this study, we investigated the ability of rMBP-NAP to induce antitumor immunity using two murine models of hepatoma H22 and sarcoma S180. Subcutaneous administration of mice with rMBP-NAP resulted in an about 40%-50% decrease of tumor growth compared with that of the control mice. Splenocytes from the tumor-bearing mice treated with rMBP-NAP showed a significant accumulation of CD4(+) IFN-γ-secreting cells, which is a cytokine profile of Th1 cells. Furthermore, intravenous injection of T2.5, toll like receptor (TLR) 2 blocking antibody, significantly recede the antitumor effect of rMBP-NAP and the production of IFN-γ induced by rMBP-NAP. Our findings indicate that potentiality of rMBP-NAP to be a candidate for the development of immunomodulatory antitumoral drugs.

  3. Apollo 11 preflight press conference

    NASA Technical Reports Server (NTRS)

    1969-01-01

    The three prime crewmen of the Apollo 11 lunar landing mission participate in a pre-flight press conference in the bldg 1 auditorium on July 5, 1969. Left to right, are Astronauts Neil A. Armstrong, commander; Edwin E. Aldrin Jr., lunar module pilot; and Michael Collins, command module pilot. The box-like enclosure surrounding the three astronauts was part of elaborate precautions in effect to reduce the possibility of exposing the crewmen to infectious disease in the preflight period.

  4. STS-97 Prelaunch Press Conference

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Bruce Buckingham, NASA Public Affairs, introduces Ron Dittemore, NASA Shuttle Program Manager, Bob Cabana, International Space Station (ISS) Manager, International Operations, NASA, Michael Vachon, Canadian Space Agency, David King, NASA Director of Shuttle Processing, and Lieutenant Ken Ferland, 45th Weather Squadron, US Air Force. They each give a brief statement on the STS-97 Endeavour mission, launch, and weather status and answer questions from the press.

  5. 7 CFR 58.421 - Press.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... openings readily cleanable. The pressure device shall be the continuous type. Press cloths shall be maintained in good repair and in a sanitary condition. Single service press cloths shall be used only once....

  6. 7 CFR 58.421 - Press.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... openings readily cleanable. The pressure device shall be the continuous type. Press cloths shall be maintained in good repair and in a sanitary condition. Single service press cloths shall be used only once....

  7. 7 CFR 58.421 - Press.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... openings readily cleanable. The pressure device shall be the continuous type. Press cloths shall be maintained in good repair and in a sanitary condition. Single service press cloths shall be used only once....

  8. STEM Summer Academy on the Navajo Reservation

    NASA Astrophysics Data System (ADS)

    Alexander, C. J.

    2012-12-01

    The US Rosetta Project is the NASA contribution to the International Rosetta Mission, an ESA cornerstone mission to comet Churyumov-Gerasimenko. While the project's outreach efforts span multi-media, and a variety of age and ethnic groups, a special emphasis has been made to find a way to provide meaningful outreach to the reservation communities. Because language preservation is an issue of urgent concern to the reservation communities, and because Rosetta, uniquely among NASA missions, has been named after the notion that keys to missing understanding of elements of the ancient past were found in the language on the original Rosetta stone, the US Rosetta Project has embarked upon outreach with a focus on STEM vocabulary in ancient US languages of the Navajo, Hopi, Ojibwe, and other tribal communities as the project expands. NASA image and science are used and described in the native language, alongside lay English and scientific English curriculum elements. Additionally, science (geology/chemistry/botany/physics) elements drawn from the reservation environment, including geomorphology, geochemistry, soil physics, are included and discussed in the native language as much as possible — with their analogs in other planetary environments (such as Mars). In this paper we will report on the most recent Summer Science Academy [2012], a four week summer course for middle school children, created in collaboration with teachers and administrators in the Chinle Unified School District. The concept of the Academy was initiated in 2011, and the first Academy was conducted shortly thereafter, in June 2011 with 14 children, 3 instructors, and a NASA teacher workshop. The community requested three topics: geology, astronomy, and botany. The 2012 Academy built on the curriculum already developed with more robust field trips, addressed to specific science topics, additional quantitative measurements and activities, with more written material for the cultural components from

  9. Optimization of hydrothermal synthesis of pure phase zeolite Na-P1 from South African coal fly ashes.

    PubMed

    Musyoka, Nicholas M; Petrik, Leslie F; Gitari, Wilson M; Balfour, Gillian; Hums, Eric

    2012-01-01

    This study was aimed at optimizing the synthesis conditions for pure phase zeolite Na-P1 from three coal fly ashes obtained from power stations in the Mpumalanga province of South Africa. Synthesis variables evaluated were: hydrothermal treatment time (12-48 hours), temperature (100-160°C) and varying molar quantities of water during the hydrothermal treatment step (H(2)O:SiO(2) molar ratio ranged between 0-0.49). The optimum synthesis conditions for preparing pure phase zeolite Na-P1 were achieved when the molar regime was 1 SiO(2): 0.36 Al(2)O(3): 0.59 NaOH: 0.49 H(2)O and ageing was done at 47°C for 48 hours. The optimum hydrothermal treatment time and temperature was 48 hours and 140°C, respectively. Fly ashes sourced from two coal-fired power plants (A, B) were found to produce nearly same high purity zeolite Na-P1 under identical conditions whereas the third fly ash (C) lead to a low quality zeolite Na-P1 under these conditions. The cation exchange capacity for the high pure phase was found to be 4.11 meq/g. These results highlight the fact that adjustment of reactant composition and presynthesis or synthesis parameters, improved quality of zeolite Na-P1 can be achieved and hence an improved potential for application of zeolites prepared from coal fly ash.

  10. Knockout targeting of the Drosophila nap1 gene and examination of DNA repair tracts in the recombination products.

    PubMed Central

    Lankenau, Susanne; Barnickel, Thorsten; Marhold, Joachim; Lyko, Frank; Mechler, Bernard M; Lankenau, Dirk-Henner

    2003-01-01

    We used ends-in gene targeting to generate knockout mutations of the nucleosome assembly protein 1 (Nap1) gene in Drosophila melanogaster. Three independent targeted null-knockout mutations were produced. No wild-type NAP1 protein could be detected in protein extracts. Homozygous Nap1(KO) knockout flies were either embryonic lethal or poorly viable adult escapers. Three additional targeted recombination products were viable. To gain insight into the underlying molecular processes we examined conversion tracts in the recombination products. In nearly all cases the I-SceI endonuclease site of the donor vector was replaced by the wild-type Nap1 sequence. This indicated exonuclease processing at the site of the double-strand break (DSB), followed by replicative repair at donor-target junctions. The targeting products are best interpreted either by the classical DSB repair model or by the break-induced recombination (BIR) model. Synthesis-dependent strand annealing (SDSA), which is another important recombinational repair pathway in the germline, does not explain ends-in targeting products. We conclude that this example of gene targeting at the Nap1 locus provides added support for the efficiency of this method and its usefulness in targeting any arbitrary locus in the Drosophila genome. PMID:12618400

  11. REM-Enriched Naps Are Associated with Memory Consolidation for Sad Stories and Enhance Mood-Related Reactivity.

    PubMed

    Gilson, Médhi; Deliens, Gaétane; Leproult, Rachel; Bodart, Alice; Nonclercq, Antoine; Ercek, Rudy; Peigneux, Philippe

    2015-12-29

    Emerging evidence suggests that emotion and affect modulate the relation between sleep and cognition. In the present study, we investigated the role of rapid-eye movement (REM) sleep in mood regulation and memory consolidation for sad stories. In a counterbalanced design, participants (n = 24) listened to either a neutral or a sad story during two sessions, spaced one week apart. After listening to the story, half of the participants had a short (45 min) morning nap. The other half had a long (90 min) morning nap, richer in REM and N2 sleep. Story recall, mood evolution and changes in emotional response to the re-exposure to the story were assessed after the nap. Although recall performance was similar for sad and neutral stories irrespective of nap duration, sleep measures were correlated with recall performance in the sad story condition only. After the long nap, REM sleep density positively correlated with retrieval performance, while re-exposure to the sad story led to diminished mood and increased skin conductance levels. Our results suggest that REM sleep may not only be associated with the consolidation of intrinsically sad material, but also enhances mood reactivity, at least on the short term.

  12. NAP (davunetide) protects primary hippocampus culture by modulating expression profile of antioxidant genes during limiting oxygen conditions.

    PubMed

    Arya, A; Meena, R; Sethy, N K; Das, M; Sharma, M; Bhargava, K

    2015-04-01

    Hypoxia is a well-known threat to neuronal cells and triggers the pathophysiological syndromes in extreme environments such as high altitudes and traumatic conditions such as stroke. Among several prophylactic molecules proven suitable for ameliorating free radical damage, NAP (an octapeptide with initial amino acids: asparagine/N, alanine/A, and proline/P) can be considered superlative, primarily due to its high permeability into brain through blood-brain barrier and observed activity at femtomolar concentrations. Several mechanisms of action of NAP have been hypothesized for its protective role during hypoxia, yet any distinct mechanism is unknown. Oxidative stress is advocated as the leading event in hypoxia; we, therefore, investigated the regulation of key antioxidant genes to understand the regulatory role of NAP in providing neuroprotection. Primary neuronal culture of rat was subjected to cellular hypoxia by limiting the oxygen concentration to 0.5% for 72 h and observing the prophylactic efficacies of 15fM NAP by conventional cell death assays using flow cytometry. We performed real-time quantitative polymerase chain reaction to comprehend the regulatory mechanism. Further, we validated the significantly regulated candidates by enzyme assays and immunoblotting. In the present study, we report that NAP regulates a major clad of cellular antioxidants and there is an involvement of more than one route of action in neuroprotection during hypoxia.

  13. REM-Enriched Naps Are Associated with Memory Consolidation for Sad Stories and Enhance Mood-Related Reactivity

    PubMed Central

    Gilson, Médhi; Deliens, Gaétane; Leproult, Rachel; Bodart, Alice; Nonclercq, Antoine; Ercek, Rudy; Peigneux, Philippe

    2015-01-01

    Emerging evidence suggests that emotion and affect modulate the relation between sleep and cognition. In the present study, we investigated the role of rapid-eye movement (REM) sleep in mood regulation and memory consolidation for sad stories. In a counterbalanced design, participants (n = 24) listened to either a neutral or a sad story during two sessions, spaced one week apart. After listening to the story, half of the participants had a short (45 min) morning nap. The other half had a long (90 min) morning nap, richer in REM and N2 sleep. Story recall, mood evolution and changes in emotional response to the re-exposure to the story were assessed after the nap. Although recall performance was similar for sad and neutral stories irrespective of nap duration, sleep measures were correlated with recall performance in the sad story condition only. After the long nap, REM sleep density positively correlated with retrieval performance, while re-exposure to the sad story led to diminished mood and increased skin conductance levels. Our results suggest that REM sleep may not only be associated with the consolidation of intrinsically sad material, but also enhances mood reactivity, at least on the short term. PMID:26729175

  14. 7 CFR 58.421 - Press.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Press. 58.421 Section 58.421 Agriculture Regulations... Plants Approved for USDA Inspection and Grading Service 1 Equipment and Utensils § 58.421 Press. The cheese press should be constructed of stainless steel and all joints welded and all surfaces, seams...

  15. 28 CFR 540.64 - Press pools.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Press pools. 540.64 Section 540.64... PERSONS IN THE COMMUNITY Contact With News Media § 540.64 Press pools. (a) The Warden may establish a press pool whenever he or she determines that the frequency of requests for interviews and...

  16. Yale University Press: Disseminating "Lux et Veritas"?

    ERIC Educational Resources Information Center

    Parrott, John B.

    2010-01-01

    America's university presses are situated within a network of over one hundred universities, learned societies, and scholarly associations. According to a pamphlet put out by the American Association of University Presses, these presses "make available to the broader public the full range and value of research generated by university faculty."…

  17. The Small Book Press: A Cultural Essential.

    ERIC Educational Resources Information Center

    Henderson, Bill

    1984-01-01

    Discussion of small literary book publishers notes works of small-press authors (Thomas Paine, Washington Irving, Walt Whitman, Mark Twain, Upton Sinclair, Anais Nin); today's outstanding presses (Creative Arts Book Company, Persea Books, Full Court Press, Reed and Cannon Company, Tuumba Books); and role of little magazines. Thirty-seven…

  18. The Burger Court and the Press.

    ERIC Educational Resources Information Center

    Higdon, Philip R.

    This report discusses recent cases involving freedom of the press that have been heard before the Burger court of the United States Supreme Court. The report discerns a trend toward treating the press like an ordinary citizen; this is a reversal of the view of the Warren court that the First Amendment creates special rights for the press so that…

  19. Attention that Indicators Receive in the Press

    ERIC Educational Resources Information Center

    Hendriks, Maria; Barzano, Giovanna; Brumana, Emanuela; Cremonesi, Claudia

    2004-01-01

    With the release of each edition of Education at a Glance, the Office for Economic Co-operation and Development (OECD) prepares a press briefing to be used for the international press release and which individual countries in preparing their national press releases can also use. Similarly, the European Union prepares a summary, not for use at the…

  20. Functional characterization of a NapA Na(+)/H(+) antiporter from Thermus thermophilus.

    PubMed

    Furrer, Esther M; Ronchetti, Mirco F; Verrey, François; Pos, Klaas M

    2007-02-06

    Na(+)/H(+) antiporters are ubiquitous membrane proteins and play an important role in cell homeostasis. We amplified a gene encoding a member of the monovalent cation:proton antiporter-2 (CPA2) family (TC 2.A.37) from the Thermus thermophilus genome and expressed it in Escherichia coli. The gene product was identified as a member of the NapA subfamily and was found to be an active Na(+)(Li(+))/H(+) antiporter as it conferred resistance to the Na(+) and Li(+) sensitive strain E. coli EP432 (DeltanhaA, DeltanhaB) upon exposure to high concentration of these salts in the growth medium. Fluorescence measurements using the pH sensitive dye 9-amino-6-chloro-2-methoxyacridine in everted membrane vesicles of complemented E. coli EP432 showed high Li(+)/H(+) exchange activity at pH 6, but marginal Na(+)/H(+) antiport activity. Towards more alkaline conditions, Na(+)/H(+) exchange activity increased to a relative maximum at pH 8, where by contrast the Li(+)/H(+) exchange activity reached its relative minimum. Substitution of conserved residues D156 and D157 (located in the putative transmembrane helix 6) with Ala resulted in the complete loss of Na(+)/H(+) activity. Mutation of K305 (putative transmembrane helix 10) to Ala resulted in a compromised phenotype characterized by an increase in apparent K(m) for Na(+) (36 vs. 7.6 mM for the wildtype) and Li(+) (17 vs. 0.22 mM), In summary, the Na(+)/H(+) antiport activity profile of the NapA type transporter of T. thermophilus resembles that of NhaA from E. coli, whereas in contrast to NhaA the T. thermophilus NapA antiporter is characterized by high Li(+)/H(+) antiport activity at acidic pH.

  1. Nap environment control considering respiration rate and music tempo by using sensor agent robot

    NASA Astrophysics Data System (ADS)

    Nakaso, Sayaka; Mita, Akira

    2015-03-01

    We propose a system that controls a nap environment considering respiration rates and music tempo by using a sensor agent robot. The proposed system consists of two sub-systems. The first sub-system measures respiration rates using optical flow. We conducted preparatory experiments to verify the accuracy of this sub-system. The experimental results showed that this sub-system can measure the respiration rates accurately despite several positional relationships. It was also shown that the accuracy could be affected by clothes, movements and light. The second sub-system we constructed was the music play sub-system that chooses music with the certain tempo corresponding to the respiration rates measured by the first sub-system. We conducted verification experiments to verify the effectiveness of this music play sub-system. The experimental results showed the effectiveness of varying music tempo based on the respiration rates in taking a nap. We also demonstrated this system in a real environment; a subject entered into the room being followed by ebioNα. When the subject was considered sleeping, ebioNα started measuring respiration rates, controlling music based on the respiration rates. As a result, we showed that this system could be realized. As a next step, we would like to improve this system to a nap environment control system to be used in offices. To realize this, we need to update the first sub-system measuring respiration rates by removing disturbances. We also need to upgrade music play sub-system considering the numbers of tunes, the kinds of music and time to change music.

  2. Halotolerant cyanobacterium Aphanothece halophytica contains NapA-type Na+/H+ antiporters with novel ion specificity that are involved in salt tolerance at alkaline pH.

    PubMed

    Wutipraditkul, Nuchanat; Waditee, Rungaroon; Incharoensakdi, Aran; Hibino, Takashi; Tanaka, Yoshito; Nakamura, Tatsunosuke; Shikata, Masamitsu; Takabe, Tetsuko; Takabe, Teruhiro

    2005-08-01

    Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium which can grow at NaCl concentrations up to 3.0 M and at pH values up to 11. The genome sequence revealed that the cyanobacterium Synechocystis sp. strain PCC 6803 contains five putative Na+/H+ antiporters, two of which are homologous to NhaP of Pseudomonas aeruginosa and three of which are homologous to NapA of Enterococcus hirae. The physiological and functional properties of NapA-type antiporters are largely unknown. One of NapA-type antiporters in Synechocystis sp. strain PCC 6803 has been proposed to be essential for the survival of this organism. In this study, we examined the isolation and characterization of the homologous gene in Aphanothece halophytica. Two genes encoding polypeptides of the same size, designated Ap-napA1-1 and Ap-napA1-2, were isolated. Ap-NapA1-1 exhibited a higher level of homology to the Synechocystis ortholog (Syn-NapA1) than Ap-NapA1-2 exhibited. Ap-NapA1-1, Ap-NapA1-2, and Syn-NapA1 complemented the salt-sensitive phenotypes of an Escherichia coli mutant and exhibited strongly pH-dependent Na+/H+ and Li+/H+ exchange activities (the highest activities were at alkaline pH), although the activities of Ap-NapA1-2 were significantly lower than the activities of the other polypeptides. Only one these polypeptides, Ap-NapA1-2, complemented a K+ uptake-deficient E. coli mutant and exhibited K+ uptake activity. Mutagenesis experiments suggested the importance of Glu129, Asp225, and Asp226 in the putative transmembrane segment and Glu142 in the loop region for the activity. Overexpression of Ap-NapA1-1 in the freshwater cyanobacterium Synechococcus sp. strain PCC 7942 enhanced the salt tolerance of cells, especially at alkaline pH. These findings indicate that A. halophytica has two NapA1-type antiporters which exhibit different ion specificities and play an important role in salt tolerance at alkaline pH.

  3. First Report of Clostridium difficile NAP1/027 in a Mexican Hospital

    PubMed Central

    Camacho-Ortiz, Adrián; López-Barrera, Daniel; Hernández-García, Raúl; Galván-De los Santos, Alejandra M.; Flores-Treviño, Samantha M.; Llaca-Díaz, Jorge M.; Garza, Héctor J. Maldonado; Bosques-Padilla, Francisco J.; Garza-González, Elvira

    2015-01-01

    Background and Objective Clostridium difficile NAP1/ribotype 027 is associated with severe disease and high mortality rates. Our aim was to determine the prevalence of NAP1/ribotype 027 among C. difficile isolates in a tertiary care hospital, and review the main clinical data. Methods We included 106 stool samples from 106 patients. Samples were tested for A&B toxins and were cultured on CCFA agar. The genes tcdA, tcdB, tcdC, cdtA, and cdtB were amplified using PCR in clinical isolates. The tcdA 3’-end deletion analysis, PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) were also performed. Stool samples that were positive for culture were tested by the GeneXpert C. difficile assay. Clinical data were collected. Results Thirty-six patients tested positive for A&B toxins; and 22 patients had positive culture for C. difficile, 14 of which tested positive for the A&B toxins and all 22 patients tested positive by the GeneXpert C. difficile assay. Risk factors included an average hospital stay of 16.1 days prior to toxin detection, average antibiotic use for 16.2 days, and a median of 3 antibiotics used. The 30-day crude mortality rate was 8.4%. Six of the 22 patients died, and 3 of those deaths were directly attributed to C. difficile infection. The majority of isolates, 90.9% (20/22), carried genes tcdB, tcdA, cdtA, and cdtB; and these strains carried the corresponding downregulator gene tcdC, with an 18-bp deletion. PFGE was performed on 17 isolates, and one main pattern was observed. Analysis of the ribotyping data showed similar results. Conclusion The above findings represent the clonal spread of C. difficile in our institution, which mainly includes the NAP1/027 strain. This is the first report of C. difficile ribotype NAP1/027 in Mexico. PMID:25915544

  4. Rotorcraft pursuit-evasion in nap-of-the-earth flight

    NASA Technical Reports Server (NTRS)

    Menon, P. K. A.; Cheng, V. H. L.; Kim, E.

    1990-01-01

    Two approaches for studying the pursuit-evasion problem between rotorcraft executing nap-of-the-earth flight are presented. The first of these employs a constant speed kinematic helicopter model, while the second approach uses a three degree of freedom point-mass model. The candidate solutions to the first differential game are generated by integrating the state-costate equations backward in time. The second problem employs feedback linearization to obtain guidance laws in nonlinear feedback form. Both approaches explicitly use the terrain profile data. Sample extremals are presented.

  5. Measurement of 19 Ne(d,n)20 Na(p) reaction at RESOLUT

    NASA Astrophysics Data System (ADS)

    Belarge, Joseph; Wiedenhover, Ingo; Baby, Lagy; Kuvin, Sean; Baker, Jessica; Blackmon, Jeff; Deibel, Catherine; Macon, Kevin; Gay, Dennis; Colbert, Kayla; Quails, Nathan

    2015-04-01

    The 19 Ne(p, γ)20 Na reaction is believed to be a link between the hot CNO cycle and the rp-process. States near the proton threshold in 20 Na play a critical role in determining the reaction rate. Most notably, a known state at 450 keV above the proton threshold has yet to be firmly assigned a spin and parity. Using a radioactive 19 Ne beam produced at the RESOLUT radioactive beam facility at FSU we have studied the 19 Ne(d,n)20 Na(p) reaction to identify the spin and parity of near proton threshold states in 20 Na.

  6. Geothermal Academy: Focus Center for Data Collection, Analysis, and Dissemination

    SciTech Connect

    Nakagawa, Masami, Ph.D.; Fujiono, Hendro, Ph.D.; McCartney, John S., Ph.D.; Reed, Adam, J.D., Esq.

    2011-10-31

    Geothermal Academy: A Pathway for Confirmation of Ground-Source Heat Pumps in the United States. In 2008, Oak Ridge National Laboratory issued a report on geothermal heats pumps (GHPs) focused on the market status, barriers to adoption, and actions to overcome these barriers (Hughes 2008). Of the barriers raised in this report, of the most pressing is the lack of performance and energy usage data for GHPs. Further, an associated barrier is a lack of a fair comparison of the energy usage of conventional heating and cooling systems for the same building. Because of these barriers, we are not able to say how much energy is used by well-designed GHP systems on a long-term basis, nor are we able to say how better their energy usage is compared to conventional systems. The need for a fair comparison with conventional systems is particularly relevant as modern versions of conventional air conditioners, gas furnaces, and boilers have also incorporated energy saving technologies. As a first step to address this barrier, the Geothermal Academy has developed a framework for data collection. This framework has already been applied to several geothermal installations in Colorado (Nakagawa etal. 2010). The framework classifies data into different categories based on the relevance of the dat to understanding the energy consumption of a GHP system. The categories are: direct energy consumption data, heat exchange performance data, and GHP design parameter data. The main recommendation of this project is to include a minimal data collection system on each heat pump installed in the U.S., capable of measuring the electrical energy consumed, the entering/exiting fluid temperatures, and circulation rates. This is a viable and cost effective solution which will provide performance data, as data collection systems are only a fraction of the cost of a GHP unit and modern GHP units already incorporate sensors to monitor energy usage and the entering and exiting fluid temperatures

  7. Histone density is maintained during transcription mediated by the chromatin remodeler RSC and histone chaperone NAP1 in vitro

    PubMed Central

    Kuryan, Benjamin G.; Kim, Jessica; Tran, Nancy Nga H.; Lombardo, Sarah R.; Venkatesh, Swaminathan; Workman, Jerry L.; Carey, Michael

    2012-01-01

    ATPases and histone chaperones facilitate RNA polymerase II (pol II) elongation on chromatin. In vivo, the coordinated action of these enzymes is necessary to permit pol II passage through a nucleosome while restoring histone density afterward. We have developed a biochemical system recapitulating this basic process. Transcription through a nucleosome in vitro requires the ATPase remodels structure of chromatin (RSC) and the histone chaperone nucleosome assembly protein 1 (NAP1). In the presence of NAP1, RSC generates a hexasome. Despite the propensity of RSC to evict histones, NAP1 reprograms the reaction such that the hexasome is retained on the template during multiple rounds of transcription. This work has implications toward understanding the mechanism of pol II elongation on chromatin. PMID:22308335

  8. 2016 Service Academy Gender Relations Survey: Overview Report

    DTIC Science & Technology

    2017-02-01

    U.S. Naval Academy); Dr. Amanda Lords and Dr. Kimberly Dickman (U.S. Air Force Academy); and Ms. Shannon Norenberg (U.S. Coast Guard Academy...Yvette Claros, Sarah De Silva, Jeffrey McLeod, Lindsay Thomas, and Amanda Barry, Fors Marsh Group, LLC, provided quality control for this report...James Khun c Joseph Luchman c Ron Vega c Natalie Namrow c Xavier Klauberg c Amanda Grifka b Quality Control Team Yvette Claros c Sarah De

  9. 2014 Service Academy Gender Relations Survey: Statistical Methodology Report

    DTIC Science & Technology

    2014-12-12

    TITLE AND SUBTITLE 2014 Service Academy Gender Relations Survey: Statistical Methodology Report 6. AUTHOR(S) Al Nassir, F., Dr.; Schneider, J., McGrath...Service Academy Gender Relations Survey (2014 SAGR), which fielded April 7,2014 through April 25,2014. In the five SAGR surveys conducted by DMDC-RSSC...0983 Standard Form 298 (Rev. 8/98) Prescribed by ANSI Std. Z39.18 2014 Service Academy Gender Relations Survey Statistical Methodology Report

  10. Dissolution kinetics of synthetic zeolite NaP1 and its implication to zeolite treatment of contaminated waters.

    PubMed

    Cama, Jordi; Ayora, Carles; Querol, Xavier; Ganor, Jiwchar

    2005-07-01

    The effect of pH on the dissolution kinetics of NaP1 zeolite, which was produced from the alkaline treatment of coal fly ash and may be used for decontamination of acid mine waters, is studied. The sample contains considerable amounts of accessory phases that partly dissolve during the experiment. Therefore, the dissolution rate was estimated during a stage in which the Al/Si ratio was equal to that of NaP1 (0.6). The release rate of these elements is controlled by the dissolution of the zeolite itself during this stage. The dissolution rate of NaP1 slows down with increasing pH in the acidic range, becomes constant at an intermediate pH, and increases with increasing pH in the basic range. The observed changes in rates were described using a rate law based on a surface speciation model. Using this rate law, we calculated the half-life of NaP1 to be about 2 years at near neutral pH and less than 10 days at pH below 3. For the utilization of NaP1 in the treatment of wastewaters or acid mine waters, these short half-lives bear two implications: (1) The treated waters must be kept at near neutral pH, and NaP1 should be added periodically to the treated waters in order to compensate for zeolite loss. (2) In water treatment applications that require a relatively short reaction time, the zeolite removed from the effluents should be kept dry in order to avoid its decomposition and the consequent release of the adsorbed metal to the environment.

  11. Enhancement of Declarative Memory Performance Following a Daytime Nap Is Contingent on Strength of Initial Task Acquisition

    PubMed Central

    Tucker, Matthew A.; Fishbein, William

    2008-01-01

    Study Objectives: In this study we examined the benefit of a daytime nap containing only NREM sleep on the performance of three declarative memory tasks: unrelated paired associates, maze learning, and the Rey-Osterrieth complex figure. Additionally, we explored the impact of factors related to task acquisition on sleep-related memory processing. To this end, we examined whether testing of paired associates during training leads to sleep-related enhancement of memory compared to simply learning the word pairs without test. We also examined whether strength of task acquisition modulates sleep-related processing for each of the three tasks. Subjects and Procedure: Subjects (11 male, 22 female) arrived at 11:30, were trained on each of the declarative memory tasks at 12:15, and at 13:00 either took a nap or remained awake in the sleep lab. After the nap period, all subjects remained in the lab until retest at 16:00. Results: Compared to subjects who stayed awake during the training-retest interval, subjects who took a NREM nap demonstrated enhanced performance for word pairs that were tested during training, but not for untested word pairs. For each of the three declarative memory tasks, we observed a sleep-dependent performance benefit only for subjects that most strongly acquired the tasks during the training session. Conclusions: NREM sleep obtained during a daytime nap benefits declarative memory performance, with these benefits being intimately tied to how well subjects acquire the tasks and the way in which the information is acquired. Citation: Tucker MA; Fishbein W. Enhancement of declarative memory performance following a daytime nap is contingent on strength of initial task acquisition. SLEEP 2008;31(2):197–203. PMID:18274266

  12. Benefits of napping and an extended duration of recovery sleep on alertness and immune cells after acute sleep restriction.

    PubMed

    Faraut, Brice; Boudjeltia, Karim Zouaoui; Dyzma, Michal; Rousseau, Alexandre; David, Elodie; Stenuit, Patricia; Franck, Thierry; Van Antwerpen, Pierre; Vanhaeverbeek, Michel; Kerkhofs, Myriam

    2011-01-01

    Understanding the interactions between sleep and the immune system may offer insight into why short sleep duration has been linked to negative health outcomes. We, therefore, investigated the effects of napping and extended recovery sleep after sleep restriction on the immune and inflammatory systems and sleepiness. After a baseline night, healthy young men slept for a 2-h night followed by either a standard 8-h recovery night (n=12), a 30-min nap (at 1 p.m.) in addition to an 8-h recovery night (n=10), or a 10-h extended recovery night (n=9). A control group slept 3 consecutive 8-h nights (n=9). Subjects underwent continuous electroencephalogram polysomnography and blood was sampled every day at 7 a.m. Leukocytes, inflammatory and atherogenesis biomarkers (high-sensitivity C-reactive protein, interleukin-8, myeloperoxidase, fibrinogen and apolipoproteins ApoB/ApoA), sleep patterns and sleepiness were investigated. All parameters remained unchanged in the control group. After sleep restriction, leukocyte and - among leukocyte subsets - neutrophil counts were increased, an effect that persisted after the 8-h recovery sleep, but, in subjects who had a nap or a 10-h recovery sleep, these values returned nearly to baseline. Inflammatory and atherogenesis biomarkers were unchanged except for higher myeloperoxidase levels after sleep restriction. The increased sleepiness after sleep restriction was reversed better in the nap and extended sleep recovery conditions. Saliva cortisol decreased immediately after the nap. Our results indicate that additional recovery sleep after sleep restriction provided by a midday nap prior to recovery sleep or a sleep extended night can improve alertness and return leukocyte counts to baseline values.

  13. Case Presentations from the Addiction Academy.

    PubMed

    Laes, JoAn R; Wiegand, Timothy

    2016-03-01

    In this article, a case-based format is used to address complex clinical issues in addiction medicine. The cases were developed from the authors' practice experience, and were presented at the American College of Medical Toxicology Addiction Academy in 2015. Section I: Drug and Alcohol Dependence and Pain explores cases of patients with co-occurring pain and substance use disorders. Section II: Legal and Policy Issues in Substance Use Disorders highlights difficult legal and policy questions in addiction medicine. Section III: Special Populations and Addictive Disorders delves into the complexity of addiction in special populations (pregnant, pediatric, and geriatric patients).

  14. The Teachers Academy for Mathematics and Science

    SciTech Connect

    Not Available

    1992-01-01

    In his State of the Union address on January 31, 1990, President Bush set a goal for US students to be number one in the world in mathematics and science achievement by the year 2000. The Teachers Academy for Mathematics and Science in Chicago is an experiment of unprecedented boldness and scale that can provide a means to the President's goal, both for the Chicago area and as a national model. This document covers organization and governance, program activities, future training goals, and evaluation programs.

  15. The Renaissance academies between science and the humanities.

    PubMed

    McNeely, Ian F

    2009-01-01

    The scientific academies of early modern Europe have long been recognized for their critical role in incubating and legitimating the Scientific Revolution. So also, academies dedicated to vernacular literature and language, opera and ballet, art and design, and speculative philosophy all contributed greatly to the emergence of new humanistic art forms. Rarely, though, have scholars asked whether a common institutional culture united the scientific and humanistic academies across disciplinary divides that "Renaissance men" famously refused to acknowledge. This essay, an interdisciplinary synthesis of existing scholarship, attempts just that, discerning a move toward practices of "objectivity" across the entire academy movement.

  16. [The National Academy of Medicine of Mexico (1836-1912)].

    PubMed

    Rodríguez Pérez, Martha Eugenia

    2013-01-01

    The article presents a summary of the development of the National Academy of Medicine of Mexico since its beginnings in 1836, when the first Academy was created, until 1912 when the organization became an official institution and an advisory authority for the Federal Government. The different stages that the Academy has gone through show the dynamism of the corporation and the activity and energy of its members. As a whole, they advanced medicine in the country at the same time as the Academy fought for the recognition, consolidation, and support of its research.

  17. Synthesis of NaP zeolite at room temperature and short crystallization time by sonochemical method.

    PubMed

    Pal, Pameli; Das, Jugal K; Das, Nandini; Bandyopadhyay, Sibdas

    2013-01-01

    NaP zeolite nano crystals were synthesized by sonochemical method at room temperature with crystallization time of 3h. For comparison, to insure the effect of sonochemical method, the hydrothermal method at conventional synthesis condition, with same initial sol composition was studied. NaP zeolites are directly formed by ultrasonic treatment without the application of autogenous pressure and also hydrothermal treatment. The effect of ultrasonic energy and irradiation time showed that with increasing sonication energy, the crystallinity of the powders decreased but phase purity remain unchanged. The synthesized powders were characterized by XRD, IR, DTA TGA, FESEM, and TEM analysis. FESEM images revealed that 50 nm zeolite crystals were formed at room temperature by using sonochemical method. However, agglomerated particles having cactus/cabbage like structure was obtained by sonochemical method followed by hydrothermal treatment. In sonochemical process, formation of cavitation and the collapsing of bubbles produced huge energy which is sufficient for crystallization of zeolite compared to that supplied by hydrothermal process for conventional synthesis. With increasing irradiation energy and time, the crystallinity of the synthesized zeolite samples increased slightly.

  18. Job demands and resting and napping opportunities for nurses during night shifts: impact on sleepiness and self-evaluated quality of healthcare.

    PubMed

    Barthe, Béatrice; Tirilly, Ghislaine; Gentil, Catherine; Toupin, Cathy

    2016-01-01

    The aim of this field study is to describe night shift resting and napping strategies and to examine their beneficial effects on sleepiness and quality of work. The study was carried out with 16 nurses working in an intensive care unit. Data collected during 20 night shifts were related to job demands (systematic observations), to the duration and timing of rests and naps taken by nurses (systematic observations, sleep diaries), to sleepiness (Karolinska Sleepiness Scale), and to quality of work scores (visual analog scale). The results showed that the number of rests and naps depended on the job demands. Resting and napping lowered the levels of sleepiness at the end of the shift. There was no direct relationship between sleepiness and the quality of work score. Discussions about the choice of indicators for the quality of work are necessary. Suggestions for implementing regulations for prescribed napping during night shifts are presented.

  19. DOD Service Academies: More Changes Needed To Eliminate Hazing. Report to Congressional Requesters.

    ERIC Educational Resources Information Center

    General Accounting Office, Washington, DC. Div. of National Security and International Affairs.

    This study sought to review the extent of hazing at the three Department of Defense (DOD) service academies, the actions taken by the academies to control and eliminate hazing, and the impact of hazing on cadets and midshipmen. The three academies studied were the Naval Academy (Annapolis, Maryland), the Air Force Academy (Colorado Springs,…

  20. Structural, electronic, sodium diffusion and elastic properties of Na-P alloy anode for Na-ion batteries: Insight from first-principles calculations

    NASA Astrophysics Data System (ADS)

    Lu, Huansheng; Xu, Bo; Shi, Jing; Wu, Musheng; Hu, Yinquan; Ouyang, Chuying

    2016-11-01

    Sodium-ion batteries (NIBs) as an alternative to lithium-ion batteries (LIBs) have recently received great attentions because of the relatively high abundance of sodium. Searching for suitable anode materials has always been a hot topic in the field of NIB study. Recent reports show that phosphorus-based materials are potential as the anode materials for NIBs. Using first-principles calculations, herein, we study the atomic and electronic structures, diffusion dynamics and intrinsic elastic properties of various Na-P alloy compounds (NaP5, Na3P11, NaP and Na3P) as the intermediate phases during Na extraction/insertion in phosphorus-based anode materials. It is found that all the crystalline phases of Na-P alloy phases considered in our study are semiconductors with band gaps larger than that of black phosphorus (BP). The calculations of Na diffusion dynamics indicate a relatively fast Na diffusion in these materials, which is important for good rate performance. In addition, the diffusion channels of sodium ions are one-dimensional in NaP5 phase and three-dimensional in other three phases (Na3P11, NaP and Na3P). Elastic constant calculations indicate that all four phases are mechanically stable. Among them, however, NaP5, Na3P11 and NaP alloy phases are ductile, while the fully sodiated phase Na3P is brittle. In order to improve the electrochemical performance of Na-P alloy anodes for NIBs, thus, promoting ductility of Na-P phase with high sodium concentration may be an effective way.

  1. CSA/ASC Press Briefing

    NASA Technical Reports Server (NTRS)

    2005-01-01

    Nicole Gignac, Senior Communications Advisor Canadian Space Agency (CSA), introduces the panel for this CSA/ASC press briefing. The panel consist of: Dr. Steve Maclean, CSA astronaut; Dr. Paul Cooper, Deputy General Manager, MacDonald, Dettwiler and Associates Ltd. (MDA); and Dr. Iain Christie, Director of Research and Development from NEPTEC. Maclean will fly on the STS-115 Mission in February of 2006. He presents pictures and talks about the crewmembers of the Space Shuttle Columbia and Space Shuttle Discovery. Maclean also discusses entry risks as it pertains to the leading edge wing of Discovery. Paul Cooper explains and shows a video presentation of Canadarm robotics. The briefing ends with Dr Christie explaining the sensitive three dimensional inspections are performed in space that looks for cracks in the leading edge wing of the Space Shuttle Discovery.

  2. Cold press sintering of simulated lunar basalt

    NASA Technical Reports Server (NTRS)

    Altemir, D. A.

    1993-01-01

    In order to predict the conditions for which the lunar regolith may be adequately sintered, experiments were conducted in which samples of simulated lunar basalt (MLS-1) were pressed at high pressures and then heated in an electric furnace. This sintering process may be referred to as cold press sintering since the material is pressed at room temperature. Although test articles were produced which possessed compressive strengths comparable to that of terrestrial concrete, the cold press sintering process requires very high press pressures and sintering temperatures in order to achieve that strength. Additionally, the prospect of poor internal heat transfer adversely affecting the quality of sintered lunar material is a major concern. Therefore, it is concluded that cold press sintering will most likely be undesirable for the production of lunar construction materials.

  3. Slurry-pressing consolidation of silicon nitride

    NASA Technical Reports Server (NTRS)

    Sanders, William A.; Kiser, James D.; Freedman, Marc R.

    1988-01-01

    A baseline slurry-pressing method for a silicon nitride material is developed. The Si3N4 composition contained 5.8 wt percent SiO2 and 6.4 wt percent Y2O3. Slurry-pressing variables included volume percent solids, application of ultrasonic energy, and pH. Twenty vol percent slurry-pressed material was approximately 11 percent stronger than both 30 vol percent slurry-pressed and dry-pressed materials. The Student's t-test showed the difference to be significant at the 99 percent confidence level. Twenty volume percent (300 h) slurry-pressed test bars exhibited strengths as high as 980 MPa. Large, columnar beta-Si3N4 grains caused failure in the highest strength specimens. The improved strength correlated with better structural uniformity as determined by radiography, optical microscopy, and image analysis.

  4. [The press and family planning].

    PubMed

    Abraham De D'ornellas, R

    1987-01-01

    The treatment in the press of family planning hinges on two fundamental factors: the taboo of the leftist groups and the taboo of the Catholic Church, whose head is against abortion under any circumstances. Leftist views insinuate that family planning is the genocidal plan of North American imperialists against the Third World and, in particular, against Latin America. This genocidal plan is supposed to subject poor populations to international schemes. In the press family planning is often treated in a sanctimonious fashion, lumping it together with topics like pornography, sex, and violence. In 1983 the daily newspaper Expreso published a supplement running every week for almost three months about the issue of population, which dealt fairly extensively with such topics as population and housing, education, employment, and urban proliferation, as well as responsible parenthood and child survival. In addition, there was a detailed description of contraceptive methods. In October 1986 another surprising thing happened: the President of Peru talked about the topic of family planning, which at the time was an act of courage. Since then much has changed; the whole world is interested in family planning and certain aspects of population. Since October 1986 more has been published in this domain than during the preceding 20 years. In contrast, the Church reacted differently to this issue: after some initial caution, the conference of Peruvian bishops attacked all methods of modern contraceptives and private institutions of family planning. The information boom in family planning will certainly continue. At the moment this flood of articles and editorials about the issue is an expression of the anxiety of families related to uncontrolled reproduction and the fear of overpopulation in large cities devoid of minimal services.

  5. NASA/MSFC Large Stretch Press Study

    NASA Technical Reports Server (NTRS)

    Choate, M. W.; Nealson, W. P.; Jay, G. C.; Buss, W. D.

    1985-01-01

    The purpose of this study was to: A. assess and document the advantages/disadvantages of a government agency investment in a large stretch form press on the order of 5000 tons capacity (per jaw); B. develop a procurement specification for the press; and C. provide trade study data that will permit an optimum site location. Tasks were separated into four major elements: cost study, user survey, site selection, and press design/procurement specification.

  6. Boot Camp for Education CEOs: The Broad Foundation Superintendents Academy

    ERIC Educational Resources Information Center

    Jehlen, Alain

    2012-01-01

    The Broad Foundation Superintendents Academy is the most prominent and most controversial training institute for school chiefs. The Academy is the flagship program of the Eli and Edythe Broad Foundation, the smallest of a triumvirate of corporate foundations that are at the heart of the billionaire campaign to remake public education in the image…

  7. Employer Involvement in Career Academies: Old Model Sparks New Involvement.

    ERIC Educational Resources Information Center

    Weil, Lindsay

    More employers have mobilized to support career academies. They say that in addition to improving student engagement in school, academies help to strengthen relationships among business leaders, educators, and civic leaders; prevent high risk students from dropping out; and expand their pipeline of qualified workers. Academic research has helped…

  8. 75 FR 54086 - Global Intellectual Property Academy Program Survey

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-03

    ... Property Academy Program Survey ACTION: Proposed collection; comment request. SUMMARY: The United States... . Include ``0651- 00xx Global Intellectual Property Academy Program Survey comment'' in the subject line of... . SUPPLEMENTARY INFORMATION I. Abstract The U.S. Patent and Trademark Office plans to survey participants of...

  9. Vocational Education in the 19th Century American Academy.

    ERIC Educational Resources Information Center

    Law, Gordon F.

    The phrase "all things useful and all things ornamental," coined by Benjamin Franklin, describes the stated mission of most of the approximately 6,000 educational academies flourishing in America in the mid-19th century. Built upon the roots of Latin grammar schools, the academies evolved to include courses in many areas, from classical…

  10. Visible Leading: Principal Academy Connects and Empowers Principals

    ERIC Educational Resources Information Center

    Hindman, Jennifer; Rozzelle, Jan; Ball, Rachel; Fahey, John

    2015-01-01

    The School-University Research Network (SURN) Principal Academy at the College of William & Mary in Williamsburg, Virginia, has a mission to build a leadership development program that increases principals' instructional knowledge and develops mentor principals to sustain the program. The academy is designed to connect and empower principals…

  11. Academy of READING®. What Works Clearinghouse Intervention Report

    ERIC Educational Resources Information Center

    What Works Clearinghouse, 2014

    2014-01-01

    "Academy of READING"® is an online program that aims to improve students' reading skills using a structured and sequential approach to learning in five core areas--phonemic awareness, phonics, fluency, vocabulary, and comprehension. The What Works Clearinghouse (WWC) identified 38 studies of "Academy of READING"® for adolescent…

  12. Evolution of a Planetary System. SETI Academy Planet Project.

    ERIC Educational Resources Information Center

    Search for Extraterrestrial Intelligence Inst., Mountain View, CA.

    The SETI Academy Planet Project provides an exciting, informative, and creative series of activities for elementary students (grades 5-6) in these activities each student plays the role of a cadet at the SETI Academy, a fictitious institution. This unit examines the evolution of stars and planets which is an important aspect of the search for…

  13. Teachers Who Write: Poems from Phillips Exeter Academy

    ERIC Educational Resources Information Center

    Independent School, 2017

    2017-01-01

    The humanities are thriving at Phillips Exeter Academy (New Hampshire), and not just in the classroom. Though Exeter teachers need not publish or perish, publish many do, in the departments of History, Religion, and most of all English. An array of published poets at Phillips Exeter Academy present excerpts of their work in this article: (1)…

  14. Preparing Administrators for Future Challenges: The AWE Leadership Academy.

    ERIC Educational Resources Information Center

    Lyman, Lawrence; And Others

    Recognizing that strong educational leadership is the pivotal point in successful school improvement programs, the Kansas school districts of Arkansas City, Winfield, and El Dorado joined together during the 1988-89 school year to establish the AWE Leadership Academy. The purpose of the academy is to provide a program of continued professional…

  15. Academic Integrity at the United States Air Force Academy

    ERIC Educational Resources Information Center

    Derby, LeAnn

    2006-01-01

    In troubled times, where threats to honor abound, it is essential that people support students who may have been enculturated in social dishonesty. The United States Air Force Academy (USAFA) has worked hard to put in place a number of supports to help cadets to grow beyond social norms where dishonesty may be tolerated. The academy seeks to…

  16. Diversity of Service Academy Entrants and Graduates. Monograph

    ERIC Educational Resources Information Center

    Kirby, Sheila Nataraj; Thie, Harry J.; Naftel, Scott; Adelson, Marisa

    2010-01-01

    In the wake of concern about the diversity of cadets/midshipmen selected by the service academies, Congress requested that the Secretary of Defense conduct a comprehensive assessment of the recruiting efforts, admissions policies, graduation rates, and career success rates of entrants and graduates at the United States Military Academy (USMA), the…

  17. A Profile of the California Partnership Academies, 2004-2005

    ERIC Educational Resources Information Center

    ConnectEd: The California Center for College and Career, 2007

    2007-01-01

    State legislation launched the California Partnership Academies (CPAs) in 1984. Now operating in more than 200 comprehensive high schools, CPAs have been used as a model for high school reform in California and elsewhere. Academies typically feature multi-age learning groups, team teaching and career-based instruction. Teachers help students…

  18. Mathematics Academies: Cohort 1 Evaluation Study, 2011-2013

    ERIC Educational Resources Information Center

    Stohr, Amber D.

    2014-01-01

    Commencing in 2011, the Mathematics Academies Initiative is a series of professional development academies (lasting 1 to 2 years, depending on cohort) with the primary objectives of (a) providing educators with a high quality professional development experience that enhances their mathematical content knowledge and pedagogical skills, and (b)…

  19. Parents in the Admission Process: A Service Academy's Perspective.

    ERIC Educational Resources Information Center

    Combs, Thomas D., Jr.

    1986-01-01

    Service academies have found that parental influence is a significant factor in the application and selection processes. Project Outreach of the Coast Guard Academy, a parent-to-parent contact using volunteers supervised by the Admissions Office, represents a way of showing concern regarding each student's uniqueness and of constructively…

  20. The Academy and the Engineering Sciences: An Unwelcome Royal Gift

    ERIC Educational Resources Information Center

    Konig, Wolfgang

    2004-01-01

    On 19 March 1900, at the bicentenary celebrations of the Prussian Academy of Sciences, Kaiser Wilhelm II established three new fellowships in engineering sciences. This was in many aspects an unwanted gift, and one which tested the Academy's relationship between pure and applied science. In the context of contemporary struggles between traditional…

  1. Academy of the Canyons Report, Fall 2000-Spring 2002.

    ERIC Educational Resources Information Center

    Meuschke, Daylene M.; Dixon, P. Scott; Gribbons, Barry C.

    Summarizes findings from an evaluation of the Academy of the Canyons, a "middle college high school" which operates on the College of the Canyons (California) campus and is open to 11th and 12th grade students whose needs are not being met by the large comprehensive high schools. This evaluation, prepared as a component of the Academy's…

  2. Parameter on comprehensive periodontal examination. American Academy of Periodontology.

    PubMed

    2000-05-01

    The American Academy of Periodontology has developed the following parameter on comprehensive periodontal examination for periodontal diseases. Appropriate screening procedures may be performed to determine the need for a comprehensive periodontal evaluation. Periodontal Screening and Recording (PSR), a screening procedure endorsed by the American Dental Association and the American Academy of Periodontology, may be utilized.

  3. Academies in Context: Politics, Business and Philanthropy and Heterarchical Governance

    ERIC Educational Resources Information Center

    Ball, Stephen J.

    2009-01-01

    The English Academies programme has become one of the most controversial aspects of the New Labour strategy for education and public sector reform. And in many ways, given the significance of the programme, that controversy is understandable and appropriate. This is particularly so because, as the author argues here, Academies are indicative of…

  4. Ethos and Vision Realization in Sponsored Academy Schools

    ERIC Educational Resources Information Center

    Gibson, Mark T.

    2015-01-01

    This article investigates the realization of ethos and vision in the early stages of sponsored academy schools in England. It is a qualitative nested case study of ten academies. Nineteen key actors were interviewed, including principals and sponsor representatives. The nests were organized by sponsor type. Key themes are discussed within the…

  5. OnTheMove Academy 2009 Organizers' Message

    NASA Astrophysics Data System (ADS)

    Spyns, Peter; Schanzenberger, Anja

    We are very happy to re-establish the tradition of organizing what is now called the OTM Academy, the workshop for coaching PhD students with promising work. The term "academy," originating from Greek antiquity, implies a strong mark of quality and excellence that is upheld and promoted by its members.

  6. USAF Academy Center for Space Situational Awareness

    NASA Astrophysics Data System (ADS)

    Dearborn, M.; Chun, F.; Liu, J.; Tippets, R.

    2011-09-01

    Since the days of Sputnik, the Air Force has maintained the surveillance of space and a position catalog of objects that can be tracked by primarily ground-based radars and optical systems. Recent events in space such as the test of the Chinese anti-satellite weapon in 2007 and the collision between an Iridium and Russian Cosmo satellite have demonstrated the great need to have a more comprehensive awareness of the situation in space. Hence space situational awareness (SSA) has become an increasingly important mission to the Air Force and to the security of the United States. To help meet the need for future leaders knowledgeable about SSA, the Air Force Academy formally stood up the Center for Space Situational Awareness (CSSAR). The goal of the CSSAR is to provide a unique combination of educational operational experience as well as a world-class research capability for hands-on education in SSA. In order to meet this goal, the CSSAR is implementing an array of sensors, operations center, and associated software, and analysis tools. For example we have radar receivers for bi-static returns from the VHF space fence, a network of small aperture telescopes, AFSPC astro standards software, and Joint Mission System software. This paper focuses on the observational capabilities of our telescopes. In general, the preferable method for characterizing a satellite is to obtain a high-resolution image. However, high-resolution images from groundbased telescopes are only achievable if the satellite is large and close in range. Thus small satellites in low-earth orbits and large satellites in geosynchronous orbits are essentially unresolved in the focal plane of a ground-based telescope. Building ever larger telescopes capable of tracking fast enough for satellites at high resolution requires tremendous resources and funding. Cost is one of the reasons we decided to develop a network of small, commercially available telescopes spatially diverse and networked together. We call

  7. Optimizing sleep/wake schedules in space: Sleep during chronic nocturnal sleep restriction with and without diurnal naps

    NASA Astrophysics Data System (ADS)

    Mollicone, Daniel J.; Van Dongen, Hans P. A.; Dinges, David F.

    2007-02-01

    Effective sleep/wake schedules for space operations must balance severe time constraints with allocating sufficient time for sleep in order to sustain high levels of neurobehavioral performance. Developing such schedules requires knowledge about the relationship between scheduled "time in bed" (TIB) and actual physiological sleep obtained. A ground-based laboratory study in N=93 healthy adult subjects was conducted to investigate physiological sleep obtained in a range of restricted sleep schedules. Eighteen different conditions with restricted nocturnal anchor sleep, with and without diurnal naps, were examined in a response surface mapping paradigm. Sleep efficiency was found to be a function of total TIB per 24 h regardless of how the sleep was divided among nocturnal anchor sleep and diurnal nap sleep periods. The amounts of sleep stages 1+2 and REM showed more complex relationships with the durations of the anchor and nap sleep periods, while slow-wave sleep was essentially preserved among the different conditions of the experiment. The results of the study indicated that when sleep was chronically restricted, sleep duration was largely unaffected by whether the sleep was placed nocturnally or split between nocturnal anchor sleep periods and daytime naps. Having thus assessed that split-sleep schedules are feasible in terms of obtaining physiological sleep, further research will reveal whether these schedules and the associated variations in the distribution of sleep stages may be advantageous in mitigating neurobehavioral performance impairment in the face of limited time for sleep.

  8. Sleep and Behavioral Correlates of Napping among Young Adults: A Survey of First-Year University Students in Madrid, Spain

    ERIC Educational Resources Information Center

    Vela-Bueno, Antonio; Fernandez-Mendoza, Julio; Olavarrieta-Bernardino, Sara; Vgontzas, Alexandros N.; Bixler, Edward O.; de la Cruz-Troca, Juan Jose; Rodriguez-Munoz, Alfredo; Olivan-Palacios, Jesus

    2008-01-01

    Objective: Between November 2002 and March 2003, the authors assessed the prevalence and correlates of napping among Spanish university students. Participants: The sample comprised 1,276 first-year university students; the mean age was 18.74 [plus or minus] 1.24 years, and 35.45% were men. Methods: The study was cross-sectional, and the students…

  9. The Role of Implied Motion in Engaging Audiences for Health Promotion: Encouraging Naps on a College Campus

    ERIC Educational Resources Information Center

    Mackert, Michael; Lazard, Allison; Guadagno, Marie; Hughes Wagner, Jessica

    2014-01-01

    Objective: Lack of sleep among college students negatively impacts health and academic outcomes. Building on research that implied motion imagery increases brain activity, this project tested visual design strategies to increase viewers' engagement with a health communication campaign promoting napping to improve sleep habits. Participants:…

  10. Inhibitory effects of rHP-NAP IgY against Helicobacter pylori attachment to AGS cell line.

    PubMed

    Borhani, Katayoun; Mohabati Mobarez, Ashraf; Khabiri, Ali Reza; Behmanesh, Mehrdad; Khoramabadi, Nima

    2016-08-01

    Helicobacter pylori is a major human pathogen related to gastric adenocarcinoma and gastroduodenal diseases. Treatment of H. pylori infections is complicated by the rise of antibiotic resistance, necessitating investigation of alternative therapies. One such alternative is passive immunization by oral administration of antibacterial immunoglobulin. In the present study, chicken immunoglobulin (IgY) was used for passive immunotherapy against a major virulence factor of H. pylori, namely recombinant HP-Nap protein. Recombinant HP-Nap was prepared and used to immunize hens. IgY was purified from the eggs by polyethylene glycol precipitation method with a total IgY-HP-NAP yield of 30 mg per egg. The inhibitory effect of specific IgY on H. pylori attachment was investigated in AGS cell line infected by the bacteria. The results demonstrate the potent effect of IgY- HP-NAP in inhibition of H. pylori attachment to the AGS cells.

  11. 76 FR 9760 - Presidential Academies for Teaching of American History and Civics; Office of Innovation and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-22

    ... Presidential Academies for Teaching of American History and Civics; Office of Innovation and Improvement; Overview Information; Presidential Academies for Teaching of American History and Civics; Notice Inviting... program supports the establishment of Presidential Academies for Teaching of American History and...

  12. [Surgery in the Academy: history, achievements and prospects].

    PubMed

    Maĭstrenko, N A; Samokhvalov, I M

    2013-12-01

    The 215th anniversary of the Imperial Medical and Surgical Academy (now Military Medical) is a reason not only for celebration, but also is the moment of truth, connecting the great history of surgical development in Russian and its prospects in 21st century. The Kirov Military Medical Academy is the center of professional education for military surgeons. There is not only military medical research and practice, but also clinical. The main goal of the Academy is development of different schools of the Academy. These schools are the fundamental basis for researches and military-medical staff training able for any service during the peace and wartime. Nowadays the Academy is not only the leading institute on battlefield surgery and treatment of severe multisystem injuries, but also the training center for emergency medicine.

  13. [The contribution of the Military Medical Academy to space exploration].

    PubMed

    Belevitin, A B; Tsygan, V N; Blaginin, A A; Kaltygin, M V

    2011-04-01

    The article examines the contribution of the Military Medical Academy as an educational, research and medical institutions in the establishment and development of the national Russian manned spaceflight. Lists the names of the Academy's scientists, working in laboratories and in the Cosmonaut Training Center. In 1958 the academy was formed Department of Aviation Medicine from the research laboratories of aviation medicine, psychophysiology, and encephalography, a division of pressure chambers and furnaces. Direct contribution to the preparation of the first manned space flight made by graduates of the academy, E.A. Karpov (the first chief of Cosmonauts Training Center. Yuri Gagarin) and I.A. Kolosov. Over the half century history of the Russian Space Military Medical Academy, prepared and sent to the CPC Gagarin more than 110 of its graduates.

  14. Science, Engineering, Mathematics and Aerospace Academy

    NASA Technical Reports Server (NTRS)

    1997-01-01

    This is an annual report on the Science, Engineering, Mathematics, and Aerospace Academy (SEMAA), which is run as a collaborative effort of NASA Lewis Research Center, and Cuyahgoga Community College. The purpose of SEMA is to increase the percentage of African Americans, and Hispanics in the fields of science and technology. The SEMAA program reaches from kindergarden, to grade 12, involving the family of under-served minorities in the education of the children. The year being reported (i.e., 1996-1997) saw considerable achievement. The program served over 1,939 students, and 120 parents were involved in various seminars. The report goes on to review the program and its implementation for each grade level. It also summarizes the participation, by gender and ethnicity.

  15. Science, Engineering, Mathematics and Aerospace Academy

    NASA Technical Reports Server (NTRS)

    1996-01-01

    The Science, Engineering, Mathematics and Aerospace Academy (SEMAA) was established in September, 1993, by Cuyahoga Community College and the NASA Lewis Research Center. Funding for SEMAA was provided by NASA Headquarters' Office of Equal Employment Opportunities. SEMAA brought together five preexisting youth programs at Cuyahoga Community College. All the programs shared the common goals of 1) Increasing the participation of underrepresented/underserved groups in science, mathematics and engineering and technology careers. 2) Increasing "success" rates of all students interested in science and mathematics. 3) Developing partnerships to recognize and support students interested in these fields. 4) Supporting continued success of highly successful students. The framework for each preexisting program allowed SEMAA to have a student population ranging from kindergarten through the twelfth-grade. This connectivness was the foundation for the many decisions which would make SEMAA a truly innovative program.

  16. Intranasal NAP (davunetide) decreases tau hyperphosphorylation and moderately improves behavioral deficits in mice overexpressing α-synuclein

    PubMed Central

    Magen, Iddo; Ostritsky, Regina; Richter, Franziska; Zhu, Chunni; Fleming, Sheila M; Lemesre, Vincent; Stewart, Alistair J; Morimoto, Bruce H; Gozes, Illana; Chesselet, Marie-Françoise

    2014-01-01

    Genome-wide association studies have identified strong associations between the risk of developing Parkinson's disease (PD) and polymorphisms in the genes encoding α-synuclein and the microtubule-associated protein tau. However, the contribution of tau and its phosphorylated form (p-tau) to α-synuclein-induced pathology and neuronal dysfunction remains controversial. We have assessed the effects of NAP (davunetide), an eight-amino acid peptide that decreases tau hyperphosphorylation, in mice overexpressing wild-type human α-synuclein (Thy1-aSyn mice), a model that recapitulates aspects of PD. We found that the p-tau/tau level increased in a subcortical tissue block that includes the striatum and brain stem, and in the cerebellum of the Thy1-aSyn mice compared to nontransgenic controls. Intermittent intranasal NAP administration at 2 μg/mouse per day, 5 days a week, for 24 weeks, starting at 4 weeks of age, significantly decreased the ratio of p-tau/tau levels in the subcortical region while a higher dose of 15 μg/mouse per day induced a decrease in p-tau/tau levels in the cerebellum. Both NAP doses reduced hyperactivity, improved habituation to a novel environment, and reduced olfactory deficits in the Thy1-aSyn mice, but neither dose improved the severe deficits of motor coordination observed on the challenging beam and pole, contrasting with previous data obtained with continuous daily administration of the drug. The data reveal novel effects of NAP on brain p-tau/tau and behavioral outcomes in this model of synucleinopathy and suggest that sustained exposure to NAP may be necessary for maximal benefits. PMID:25505609

  17. Napping-Ultra Flash Profile as a Tool for Category Identification and Subsequent Model System Formulation of Caramel Corn Products.

    PubMed

    Mayhew, Emily; Schmidt, Shelly; Lee, Soo-Yeun

    2016-07-01

    In a novel approach to formulation, the flash descriptive profiling technique Napping-Ultra Flash Profile (Napping-UFP) was used to characterize a wide range of commercial caramel corn products. The objectives were to identify product categories, develop model systems based on product categories, and correlate analytical parameters with sensory terms generated through the Napping-UFP exercise. In one 2 h session, 12 panelists participated in 4 Napping-UFP exercises, describing and grouping, on a 43×56 cm paper sheet, 12 commercial caramel corn samples by degree of similarity, globally and in terms of aroma-by-mouth, texture, and taste. The coordinates of each sample's placement on the paper sheet and descriptive terms generated by the panelists were used to conduct Multiple Factor Analysis (MFA) and hierarchical clustering of the samples. Strong trends in the clustering of samples across the 4 Napping-UFP exercises resulted in the determination of 3 overarching types of commercial caramel corn: "small-scale dark" (typified by burnt, rich caramel corn), "large-scale light" (typified by light and buttery caramel corn), and "large-scale dark" (typified by sweet and molasses-like caramel corn). Representative samples that best exemplified the properties of each category were used as guides in the formulation of 3 model systems that represent the spread of commercial caramel corn products. Analytical testing of the commercial products, including aw measurement, moisture content determination, and thermal characterization via differential scanning calorimetry, were conducted and results related to sensory descriptors using Spearman's correlation.

  18. Assessing the benefits of napping and short rest breaks on processing speed in sleep-restricted adolescents.

    PubMed

    Lim, Julian; Lo, June C; Chee, Michael W L

    2017-04-01

    Achievement-oriented adolescents often study long hours under conditions of chronic sleep restriction, adversely affecting cognitive function. Here, we studied how napping and rest breaks (interleaved off-task periods) might ameliorate the negative effects of sleep restriction on processing speed. Fifty-seven healthy adolescents (26 female, age = 15-19 years) participated in a 15-day live-in protocol. All participants underwent sleep restriction (5 h time-in-bed), but were then randomized into two groups: one of these groups received a daily 1-h nap opportunity. Data from seven of the study days (sleep restriction days 1-5, and recovery days 1-2) are reported here. The Blocked Symbol Decoding Test, administered once a day, was used to assess time-on-task effects and the effects of rest breaks on processing speed. Controlling for baseline differences, participants who took a nap demonstrated faster speed of processing and greater benefit across testing sessions from practice. These participants were also affected significantly less by time-on-task effects. In contrast, participants who did not receive a nap benefited more from the rest breaks that were permitted between blocks of the test. Our results indicate that napping partially reverses the detrimental effects of sleep restriction on processing speed. However, rest breaks have a greater effect as a countermeasure against poor performance when sleep pressure is higher. These data add to the growing body of evidence showing the importance of sleep for good cognitive functioning in adolescents, and suggest that more frequent rest breaks might be important in situations where sleep loss is unavoidable.

  19. Intranasal NAP (davunetide) decreases tau hyperphosphorylation and moderately improves behavioral deficits in mice overexpressing α-synuclein.

    PubMed

    Magen, Iddo; Ostritsky, Regina; Richter, Franziska; Zhu, Chunni; Fleming, Sheila M; Lemesre, Vincent; Stewart, Alistair J; Morimoto, Bruce H; Gozes, Illana; Chesselet, Marie-Françoise

    2014-10-01

    Genome-wide association studies have identified strong associations between the risk of developing Parkinson's disease (PD) and polymorphisms in the genes encoding α-synuclein and the microtubule-associated protein tau. However, the contribution of tau and its phosphorylated form (p-tau) to α-synuclein-induced pathology and neuronal dysfunction remains controversial. We have assessed the effects of NAP (davunetide), an eight-amino acid peptide that decreases tau hyperphosphorylation, in mice overexpressing wild-type human α-synuclein (Thy1-aSyn mice), a model that recapitulates aspects of PD. We found that the p-tau/tau level increased in a subcortical tissue block that includes the striatum and brain stem, and in the cerebellum of the Thy1-aSyn mice compared to nontransgenic controls. Intermittent intranasal NAP administration at 2 μg/mouse per day, 5 days a week, for 24 weeks, starting at 4 weeks of age, significantly decreased the ratio of p-tau/tau levels in the subcortical region while a higher dose of 15 μg/mouse per day induced a decrease in p-tau/tau levels in the cerebellum. Both NAP doses reduced hyperactivity, improved habituation to a novel environment, and reduced olfactory deficits in the Thy1-aSyn mice, but neither dose improved the severe deficits of motor coordination observed on the challenging beam and pole, contrasting with previous data obtained with continuous daily administration of the drug. The data reveal novel effects of NAP on brain p-tau/tau and behavioral outcomes in this model of synucleinopathy and suggest that sustained exposure to NAP may be necessary for maximal benefits.

  20. Directional handling qualities requirements for nap of the earth (NOE) tasks

    NASA Technical Reports Server (NTRS)

    Bivens, C. C.

    1985-01-01

    A piloted simulator experiment has been conducted to ascertain the directional axis handling requirements of hover and below 40-kt tasks of a scout/attack helicopter, with attention to such light helicopter configurations as those being considered for the 'LHX' program. Two types of yaw stability and control augmentation system were implemented: one consisted of a washed-out yaw rate feedback and shaped control unit, while the other involved a yaw rate command heading-hold system. Experiment results indicate that rotorcraft configurations with high directional gust sensitivity require greater minimum yaw damping to maintain satisfactory handling qualities in nap of the earth flight. Yaw damping and control response are critical handling qualities parameters in air-to-air target acquisition and tracking.

  1. VALIDATION OF AN AUTOMATED WIRELESS SYSTEM FOR SLEEP MONITORING DURING DAYTIME NAPS

    PubMed Central

    Cellini, Nicola; McDevitt, Elizabeth A.; Ricker, Ashley A.; Rowe, Kelly M.; Mednick, Sara C.

    2017-01-01

    An automated wireless system (WS) for sleep monitoring was recently developed and validated for assessing nighttime sleep. Here, we aimed to evaluate the validity of the WS to correctly monitor daytime sleep during naps compared to polysomnography (PSG). We found that the WS underestimated wake, sleep onset latency, wake after sleep onset, and overestimated total sleep time, sleep efficiency and duration of REM sleep. Sensitivity was moderate for wake (58.51%) and light sleep (66.92%) and strong for deep sleep (83.46%) and REM sleep (82.12%). These results demonstrated that the WS had a low ability to detect wake and systematically over-scored REM sleep, implicating the WS as an inadequate substitute for PSG in diagnosing sleep disorders or for research in which sleep staging is essential. PMID:24564261

  2. Optimal guidance with obstacle avoidance for nap-of-the-earth flight

    NASA Technical Reports Server (NTRS)

    Pekelsma, Nicholas J.

    1988-01-01

    The development of automatic guidance is discussed for helicopter Nap-of-the-Earth (NOE) and near-NOE flight. It deals with algorithm refinements relating to automated real-time flight path planning and to mission planning. With regard to path planning, it relates rotorcraft trajectory characteristics to the NOE computation scheme and addresses real-time computing issues and both ride quality issues and pilot-vehicle interfaces. The automated mission planning algorithm refinements include route optimization, automatic waypoint generation, interactive applications, and provisions for integrating the results into the real-time path planning software. A microcomputer based mission planning workstation was developed and is described. Further, the application of Defense Mapping Agency (DMA) digital terrain to both the mission planning workstation and to automatic guidance is both discussed and illustrated.

  3. Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification.

    PubMed

    Flanagan, Anne-Marie; Stavenschi, Elena; Basavaraju, Shivakumar; Gaboriau, David; Hoey, David A; Morrison, Ciaran G

    2017-03-15

    Duplication of the centrosomes is a tightly regulated process. Abnormal centrosome numbers can impair cell division and cause changes in how cells migrate. Duplicated centrosomes are held together by a proteinaceous linker made up of rootletin filaments anchored to the centrioles by C-NAP1. This linker is removed in a NEK2A kinase-dependent manner as mitosis begins. To explore C-NAP1 activities in regulating centrosome activities, we used genome editing to ablate it. C-NAP1-null cells were viable and had an increased frequency of premature centriole separation, accompanied by reduced density of the centriolar satellites, with reexpression of C-NAP1 rescuing both phenotypes. We found that the primary cilium, a signaling structure that arises from the mother centriole docked to the cell membrane, was intact in the absence of C-NAP1, although components of the ciliary rootlet were aberrantly localized away from the base of the cilium. C-NAP1-deficient cells were capable of signaling through the cilium, as determined by gene expression analysis after fluid flow-induced shear stress and the relocalization of components of the Hedgehog pathway. Centrosome amplification induced by DNA damage or by PLK4 or CDK2 overexpression was markedly reduced in the absence of C-NAP1. We conclude that centriole splitting reduces the local density of key centriolar precursors to impede overduplication.

  4. Expression of an AtNAP gene homolog in senescing morning glory (Ipomoea nil) petals of two cultivars with a different flower life span.

    PubMed

    Shinozaki, Yoshihito; Tanaka, Toshimitsu; Ogiwara, Isao; Kanekatsu, Motoki; van Doorn, Wouter G; Yamada, Tetsuya

    2014-05-01

    AtNAP, a NAC family transcription factor, has been shown to promote leaf senescence in Arabidopsis. We isolated an AtNAP homolog in morning glory (Ipomoea nil), designated InNAP, and investigated its expression during petal senescence. We used two cultivars, one showing a normal short flower life span (cv. Peking Tendan) and another a longer life span (cv. Violet). InNAP was highly expressed in both cultivars. Expression was high before that of the senescence marker gene InSAG12. InNAP and InSAG12 expression was high in cv. Peking Tendan before cv. Violet. The expression of both genes was therefore temporally related to the onset of the visible senescence symptoms. An inhibitor of ethylene action (silver thiosulphate, STS) delayed petal senescence in cv. Peking Tendan but had no effect in cv. Violet. STS treatment had no clear effect on the InNAP expression in petals of both cultivars, suggesting that endogenous ethylene may not be necessary for its induction. These data suggest the hypothesis that InNAP plays a role in petal senescence, independent of the role of endogenous ethylene.

  5. Treatment with anti-NAP monoclonal antibody reduces disease severity in murine model of novel angiogenic protein-induced or ovalbumin-induced arthritis.

    PubMed

    Nataraj, N B; Krishnamurthy, J; Salimath, B P

    2013-02-01

    Rheumatoid arthritis (RA) is a polyarticular inflammatory, angiogenic disease. Synovial angiogenesis contributes to inflammation in RA. In this study we have developed an arthritic model in rats using a novel angiogenic protein (NAP), isolated from human synovial fluid of RA patients. We produced anti-NAP monoclonal antibodies (mAbs) and investigated the therapeutic efficacy of the same in adjuvant-induced or NAP-induced arthritis as a model of human RA. The treatment of arthritic rats with anti-NAP mAbs resulted in effective amelioration of paw oedema, radiological arthritic characteristics, serum levels of vascular endothelial growth factor (VEGF) and NAP, compared to that of untreated arthritic animals. Further, profiling of angiogenic markers such as synovial microvessel density, angiogenesis, CD31, VEGF and fms-like tyrosine kinase (Flt1) by immunohistochemistry both in arthritic and anti-NAP mAb-treated animals revealed the efficacy of mAb as an anti-angiogenic functional antibody. Therefore, NAP may be an attractive target to design anti-angiogenic and anti-arthritic therapies to control the pathogenesis of arthritis.

  6. Morning REM Sleep Naps Facilitate Broad Access to Emotional Semantic Networks

    PubMed Central

    Carr, Michelle; Nielsen, Tore

    2015-01-01

    Study Objectives: The goals of the study were to assess semantic priming to emotion and nonemotion cue words using a novel measure of associational breadth for participants who either took rapid eye movement (REM) or nonrapid eye movement (NREM) naps or who remained awake, and to assess the relation of priming to REM sleep consolidation and REM sleep inertia effects. Design: The associational breadth task was applied in both a priming condition, where cue words were signaled to be memorized prior to sleep (primed), and a nonpriming condition, where cue words were not memorized (nonprimed). Cue words were either emotional (positive, negative) or nonemotional. Participants were randomly assigned to either an awake (WAKE) or a sleep condition, which was subsequently split into NREM or REM groups depending on stage at awakening. Setting: Hospital-based sleep laboratory. Participants: Fifty-eight healthy participants (22 male) ages 18 to 35 y (mean age = 23.3 ± 4.08 y). Measurements and Results: The REM group scored higher than the NREM or WAKE groups on primed, but not nonprimed emotional cue words; the effect was stronger for positive than for negative cue words. However, REM time and percent correlated negatively with degree of emotional priming. Priming occurred for REM awakenings but not for NREM awakenings, even when the latter sleep episodes contained some REM sleep. Conclusions: Associational breadth may be selectively consolidated during REM sleep for stimuli that have been tagged as important for future memory retrieval. That priming decreased with REM time and was higher only for REM sleep awakenings is consistent with two explanatory REM sleep processes: REM sleep consolidation serving emotional downregulation and REM sleep inertia. Citation: Carr M, Nielsen T. Morning REM sleep naps facilitate broad access to emotional semantic networks. SLEEP 2015;38(3):433–443. PMID:25409100

  7. National Academy of Sciences Recommends Continued Support of ALMA Project

    NASA Astrophysics Data System (ADS)

    2000-05-01

    A distinguished panel of scientists today announced their support for the continued funding of the Atacama Large Millimeter Array (ALMA) Project at a press conference given by the National Academy of Sciences. The ALMA Project is an international partnership between U.S. and European astronomy organizations to build a complete imaging telescope that will produce astronomical images at millimeter and submillimeter wavelengths. The U.S. partner is the National Science Foundation, through Associated Universities, Inc., (AUI), led by Dr. Riccardo Giacconi, and the National Radio Astronomy Observatory (NRAO). "We are delighted at this show of continued support from our peers in the scientific community," said Dr. Robert Brown, ALMA U.S. Project Director and Deputy Director of NRAO. "The endorsement adds momentum to the recent strides we've made toward the building of this important telescope." In 1998, the National Research Council, the working arm of the National Academy of Sciences, charged the Astronomy and Astrophysics Survey Committee to "survey the field of space- and ground-based astronomy and astrophysics" and to "recommend priorities for the most important new initiatives of the decade 2000-2010." In a report released today, the committee wrote that it "re-affirms the recommendations of the 1991 Astronomy and Astrophysics Survey Committee by endorsing the completion of . . . the Millimeter Array (MMA, now part of the Atacama Large Millimeter Array)." In the 1991 report "The Decade of Discovery," a previous committee chose the Millimeter Array as one of the most important projects of the decade 1990-2000. Early last year, the National Science Foundation signed a Memorandum of Understanding with a consortium of European organizations that effectively merged the MMA Project with the European Large Southern Array project. The combined project was christened the Atacama Large Millimeter Array. ALMA, expected to consist of 64 antennas with 12-meter diameter dishes

  8. Daytime napping and the risk of all-cause and cause-specific mortality: a 13-year follow-up of a British population.

    PubMed

    Leng, Yue; Wainwright, Nick W J; Cappuccio, Francesco P; Surtees, Paul G; Hayat, Shabina; Luben, Robert; Brayne, Carol; Khaw, Kay-Tee

    2014-05-01

    Epidemiologic studies have reported conflicting results on the relationship between daytime napping and mortality risk, and there are few data on the potential association in the British population. We investigated the associations between daytime napping and all-cause or cause-specific mortality in the European Prospective Investigation Into Cancer-Norfolk study, a British population-based cohort study. Among the 16,374 men and women who answered questions on napping habits between 1998 and 2000, a total of 3,251 died during the 13-year follow-up. Daytime napping was associated with an increased risk of all-cause mortality (for napping less than 1 hour per day on average, hazard ratio = 1.14, 95% confidence interval: 1.02, 1.27; for napping 1 hour or longer per day on average, hazard ratio = 1.32, 95% confidence interval: 1.04, 1.68), independent of age, sex, social class, educational level, marital status, employment status, body mass index, physical activity level, smoking status, alcohol intake, depression, self-reported general health, use of hypnotic drugs or other medications, time spent in bed at night, and presence of preexisting health conditions. This association was more pronounced for death from respiratory diseases (for napping less than 1 hour, hazard ratio = 1.40, 95% confidence interval: 0.95, 2.05; for napping 1 hour or more, hazard ratio = 2.56, 95% confidence interval: 1.34, 4.86) and in individuals 65 years of age or younger. Excessive daytime napping might be a useful marker of underlying health risk, particularly of respiratory problems, especially among those 65 years of age or younger. Further research is required to clarify the nature of the observed association.

  9. Linguistic Cohabitation: Frenglish in the Mauritius Press.

    ERIC Educational Resources Information Center

    Miles, William F. S.

    1998-01-01

    Discusses the use of Frenglish on the island of Mauritius. An examination of the local press over a nine-month period turned up ten categories of Frenglish. Frenglish in the Mauritian press highlights the uniqueness of local society by linguistically contextualizing broad ranges of social, economic, political, legal, and administrative life.…

  10. Teenagers as Victims in the Press

    ERIC Educational Resources Information Center

    Andersson, Gunvor; Lundstrom, Tommy

    2007-01-01

    Research into press reporting on young people has tended to concentrate on young people as offenders. In contrast, this article focuses on press coverage of teenagers as victims. Reports in two Swedish newspapers (a morning broadsheet and an evening tabloid) were studied over a period of four months and subjected to a qualitative analysis of…

  11. Fracture toughness of hot-pressed beryllium

    NASA Technical Reports Server (NTRS)

    Lemon, D. D.; Brown, W. F., Jr.

    1985-01-01

    This paper presents the results of an investigation into the fracture toughness, sustained-load flaw growth, and fatigue-crack propagation resistance of S200E hot-pressed beryllium at room temperature. It also reviews the literature pertaining to the influence of various factors on the fracture toughness of hot-pressed beryllium determined using fatigue-cracked specimens.

  12. Freedom of the Press vs. Public Access.

    ERIC Educational Resources Information Center

    Schmidt, Benno C., Jr.

    This book surveys the implications of freedom of the press for a constitutionally rooted public right of access to electronic and print media. Part one provides general perspectives on access to the media, including discussions of access in relation to the Supreme Court, to First Amendment history and theory, to current perceptions of the press,…

  13. In the Stocks: Perilous Press Releases.

    ERIC Educational Resources Information Center

    Morton, Linda P.; Loving, Bill

    1994-01-01

    Reviews some of the legal responsibilities of public relations practitioners in the preparation of press releases. Discusses legal criteria for judging the fraudulence of press releases and discusses the timeframe for fraudulent action. Concludes with lessons that practitioners need to understand. (SR)

  14. STS-99 Prelaunch Press Briefing

    NASA Technical Reports Server (NTRS)

    2000-01-01

    The primary objective of the STS-99 mission was to complete high resolution mapping of large sections of the Earth's surface using the Shuttle Radar Topography Mission (SRTM), a specially modified radar system. This radar system produced unrivaled 3-D images of the Earth's Surface. The mission was launched at 12:31 on February 11, 2000 onboard the space shuttle Endeavour, and led by Commander Kevin Kregel. The crew was Pilot Dominic L. Pudwill Gorie and Mission Specialists Janet L. Kavandi, Janice E. Voss, Mamoru Mohri from the National Space Development Agency (Japanese Space Agency), and Gerhard P. J. Thiele from DARA (German Space Agency). This videotape shows a press briefing about a mechanical problem that the shuttle was having. There was discussion about possibly scrubbing the launch due to the problem with the Enhanced Master Events Controller. A problem with a fuel pump part had also become evident and there was discussion about the impact that this could have on the flight.

  15. A comparison of impulse drying to double felted pressing on pilot- scale shoe presses and roll presses. Progress report, No. 6

    SciTech Connect

    Orloff, D.I.

    1992-08-01

    Pilot-scale shoe press and roll press experiments have been conducted to compare impulse drying and double felted pressing. Both ceramic coated and Beloit Type C press rolls have been evaluated. The experiments show that impulse drying can provide significantly higher outgoing solids than double felled pressing at the same impulse. For example, at an impulse of 0.234 MPa seconds (34 psi seconds), sheets at an ingoing solids of 52% were impulse dried (using the Beloit Type C press roll) to 68% solids while optimized double felled pressing could only yield press dryness of, at most, 60%.

  16. Van Allen Probes Mission Space Academy: Educating middle school students about Earth's mysterious radiation belts

    NASA Astrophysics Data System (ADS)

    Butler, L.; Turney, D.; Matiella Novak, A.; Smith, D.; Simon, M.

    2013-12-01

    How's the weather in space? Why on Earth did NASA send two satellites above Earth to study radiation belts and space weather? To learn the answer to questions about NASA's Van Allen Probes mission, 450 students and their teachers from Maryland middle schools attended Space Academy events highlighting the Van Allen Probes mission. Sponsored by the Applied Physics Laboratory (APL) and Discovery Education, the events are held at the APL campus in Laurel, MD. Space Academies take students and teachers on behind-the-scenes exploration of how spacecraft are built, what they are designed to study, and introduces them to the many professionals that work together to create some of NASA's most exciting projects. Moderated by a public relations representative in the format of an official NASA press conference, the daylong event includes a student press conference with students as reporters and mission experts as panelists. Lunch with mission team members gives students a chance to ask more questions. After lunch, students don souvenir clean room suits, enjoy interactive science demonstrations, and tour APL facilities where the Van Allen Probes were built and tested before launch. Students may even have an opportunity to peek inside a clean room to view spacecraft being assembled. Prior to the event, teachers are provided with classroom activities, lesson plans, and videos developed by APL and Discovery Education to help prepare students for the featured mission. The activities are aligned to National Science Education Standards and appropriate for use in the classroom. Following their visit, student journalists are encouraged to write a short article about their field trip; selections are posted on the Space Academy web site. Designed to engage, inspire, and influence attitudes about space science and STEM careers, Space Academies provide an opportunity to attract underserved populations and emphasize that space science is for everyone. Exposing students to a diverse group of

  17. The USAF Academy Flywheel-Electric Car Technical Report.

    DTIC Science & Technology

    1980-12-01

    A--AC95 130 FRANK .J SEILER RESEARCH LAB UNITED STATES AIR FORCE A--ETC F/B 13/b THE UJSAF ACADEMY FLYWHEEL-ELECTRIC CAR TECHNICAL REPORT.1 DEC 80 R...FEB 1 8 1981 I;,) E THE USAF ACADEMY FLYWHEEL-ELECTRIC CAR TECHNICAL REPORT CAPTAIN ROBERT G. SCHWEIN., JR. PROJECT 2303 DISIUTION STTEME- A Approved...The research was conducted under Project Work Unit NLuber 2303-F-3, The USAF Academy Flywheel-Electric Car Technical Report. Captain Robert G. Schwein

  18. 29 CFR 1910.217 - Mechanical power presses.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... OCCUPATIONAL SAFETY AND HEALTH STANDARDS Machinery and Machine Guarding § 1910.217 Mechanical power presses. (a... this section. (5) Excluded machines. Press brakes, hydraulic and pneumatic power presses, bulldozers, hot bending and hot metal presses, forging presses and hammers, riveting machines and similar types...

  19. 29 CFR 1910.217 - Mechanical power presses.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... OCCUPATIONAL SAFETY AND HEALTH STANDARDS Machinery and Machine Guarding § 1910.217 Mechanical power presses. (a... this section. (5) Excluded machines. Press brakes, hydraulic and pneumatic power presses, bulldozers, hot bending and hot metal presses, forging presses and hammers, riveting machines and similar types...

  20. 29 CFR 1910.217 - Mechanical power presses.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... OCCUPATIONAL SAFETY AND HEALTH STANDARDS Machinery and Machine Guarding § 1910.217 Mechanical power presses. (a... this section. (5) Excluded machines. Press brakes, hydraulic and pneumatic power presses, bulldozers, hot bending and hot metal presses, forging presses and hammers, riveting machines and similar types...

  1. 29 CFR 1910.217 - Mechanical power presses.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... OCCUPATIONAL SAFETY AND HEALTH STANDARDS Machinery and Machine Guarding § 1910.217 Mechanical power presses. (a... this section. (5) Excluded machines. Press brakes, hydraulic and pneumatic power presses, bulldozers, hot bending and hot metal presses, forging presses and hammers, riveting machines and similar types...

  2. The influence of training age on the annual development of physical qualities within academy rugby league players.

    PubMed

    Till, Kevin; Darrall-Jones, Joshua; Weakley, Jonathon; Roe, Greg; Jones, Ben

    2016-07-15

    Previous research in academy rugby league players has evaluated the development of physical qualities according to chronological age. However, no study has considered the training age, defined as the number of formalized years of strength and conditioning training, of these players. Therefore, the purpose of this study was to present and compare the annual changes in physical qualities of academy rugby league players according to training age. Sixty-one academy players undertook a fitness testing assessment including anthropometric (height, body mass, sum of four skinfolds) and physical (10 and 20m sprint, 10m momentum, vertical jump, Yo-Yo intermittent recovery test level 1 [Yo-Yo IRTL1], one-repetition maximum [1-RM] squat, bench press and prone row) measures at the start of pre-season on two consecutive annual occasions. Players were categorized into one of three training age groups (i.e., 0, 1 or 2 years) and were analyzed using magnitude-based inferences. Almost certain, very likely or likely annual improvements were identified for body mass, 10m momentum, Yo-Yo IRTL1, vertical jump and all strength measures for the three training age groups. When training age groups were compared, 1 years showed possibly or likely lower strength increases than 0 years training age. However, the 2 years training age group demonstrated possibly or likely increased strength changes compared to 1 years. These findings suggest that training age is an important consideration for strength and conditioning practitioners but it is likely to be a combination of chronological age, biological maturity and training experience alongside dynamic inter-player variability that influences the physical development of academy rugby league players.

  3. The European Academy of Tumor Immunology

    PubMed Central

    Kroemer, Guido; Fridman, Wolf Hervé; Sautès-Fridman, Catherine; Zitvogel, Laurence

    2012-01-01

    The European Academy of Tumor Immunology (EATI, official website: http://eati.landesbioscience.com/index.html) has been founded in 2011 with the idea of creating a novel organization that responds to the need of structuring the European research space in this expanding, clinically ever more important area of research. Rapidly, this initiative, which regroups (part of) the elite of tumor immunologists, has been joined by 110 scientists, who accepted to join EATI as founding members. Obviously, EATI will not enter in competition with existing prestigious organizations, be they supranational (such as the Cancer Research Institute, CRI; the European Society for Cancer Immunology and Immunotherapy, ESCII; and the Society for the Immunotherapy of Cancer, SITC), or national [such as the Cancer Immunology Working Group, CIMM, of the American Association for Cancer Reserch; the (German) Association for Cancer Immunotherapy, CIMT; the (US) Cancer Immunotherapy Consortium, CIC; the (US) Cancer Vaccine Consortium, CVC; and the Italian Network for Cancer Biotherapy, NIBIT]. The choice of cooperation (rather than competition) with these organizations is clearly documented by the fact that many prominent members of CIMM, CIC, CIMT, CRI, CVC, ESCII, NIBIT and SITC are also EATI Academicians. PMID:22720233

  4. Khan Academy: the world's free virtual school

    NASA Astrophysics Data System (ADS)

    Dijksman, Joshua A.; Khan, Salman

    2011-03-01

    Khan Academy offers an unprecedented set of educational material for science and math education, in the form of short, free, publicly available video clips. With a growing set of already over 2000 videos, it is easily the most exhaustive collection of structured educational material on the Internet. The content is made in digestible 10-20 minute chunks; the granular nature of the material allows learners to fill in almost any of their knowledge ``gaps.'' Importantly, the conversational style used in the videos offers a fresh, new perspective on math and science instruction. With our 2 M funding grant from Google and support from the Gates foundation, we envision covering all topics that would appear in typical high-school or collegiate-level Math and Science courses, and translating these videos to the major languages across the globe. Moreover, we also offer a free and fully integrated assessment system, which allows students to practice problems at their own pace and focus on the appropriate instruction to fill in their individual gaps. Many testimonials have already proven our methods to be a highly successful educational tool. Our goal is to allow educators to improve their teaching, but above all to bring simple, rewarding and enjoyable education to the minds of many young students. Supported by Google, the Gates Foundation, donors and volunteers.

  5. STS-8 onboard crew press conference

    NASA Technical Reports Server (NTRS)

    1983-01-01

    Six news reporters listen to a response from Astronaut Guion S. Bluford (note TV monitor) in a rare space-to-Earth press conference involving all the STS-8 crew. The participants are, left to right, Gary Schwitzer, Cable News Network; Morton Dean, CBS; Roy Neal, NBC; Lynn Sherr, ABC; Howard Benedict, Associated Press; Al Rossiter, United Press International. The astronauts on the monitor are Richard H. Truly, cneter left, crew commander; Daniel C. Brandenstein, lower left, pilot; and Dr. William E. Thornton, upper left, Guion S. Bluford, upper right; and Dale E. Gardner, all mission specialists.

  6. U.S. Air Force Academy NPDES Permit

    EPA Pesticide Factsheets

    Under NPDES permit CO-0020974, the U.S. Air Force Academy is authorized to discharge from its wastewater treatment facility in El Paso County, Colorado, to Non-Potable Reservoir No. 1 on Lehman Run and to Monument Creek.

  7. American Academy of Otolaryngology--Head and Neck Surgery

    MedlinePlus

    ... of Use © Copyright 2017. American Academy of Otolaryngology — Head and Neck Surgery 1650 Diagonal Rd Alexandria, VA 22314 tel (703) 836-4444 Contact Us Privacy Policy Terms of Use r o q p k Top

  8. An Academy of Peace: Training for a Peaceful Future.

    ERIC Educational Resources Information Center

    Matsunaga, Spark M.

    1985-01-01

    The best and brightest young people of the world should have a place to learn the art of peacemaking. A U.S. Academy of Peace that would train Americans in the peaceful resolution of conflict is proposed. (RM)

  9. Peace and Conflict Resolution: A New United States Academy?

    ERIC Educational Resources Information Center

    Matsunaga, Spark M.

    1983-01-01

    A United States senator chronicles the history of efforts at the federal level to establish a national academy of peace. Results and recommendations of a recent commission study are summarized and action is urged. (MSE)

  10. The educational responsibilities of a National Academy of Medicine.

    PubMed

    Walton, H

    1994-01-01

    Academies of Medicine now should see their educational mission as concern with all stages of medical education in the nation. Traditionally, National Academies have tended to regard Postgraduate Training as their proper educational responsibility. Such limitation is no longer defensible, particularly because medical education is now accepted as a continuum and, moreover, is viewed as lifelong. Discontinuities between basic education, specialist training and continuing education are now considered detrimental, and have to be eliminated. The Medical School Curriculum must be required to equip future doctors with the self-learning skills now essential for specialist training. The longest and most critically important phase of training has unquestionably become continuing medical education. It is argued therefore that basic medical education, specialist training and continuing education have all to be concerns of National Academies. National Academies in Europe, inevitably, have come to have profound international obligations now that free movement of doctors is legally established in the European Community.

  11. Research at the Intersection of the Physical and Life Sciences, Washington, D.C.: National Academies Press, 2010.

    SciTech Connect

    Committee on Forefronts of Science at the Interface of Physical and Life Sciences

    2009-11-01

    Traditionally, the natural sciences have been divided into two branches: the biological sciences and the physical sciences. Today, an increasing number of scientists are addressing problems lying at the intersection of the two. These problems are most often biological in nature, but examining them through the lens of the physical sciences can yield exciting results and opportunities. For example, one area producing effective cross-discipline research opportunities centers on the dynamics of systems. Equilibrium, multi-stability, and stochastic behavior--concepts familiar to physicists and chemists--are now being used to tackle issues associated with living systems such as adaptation, feedback, and emergent behavior. Research at the Intersection of the Physical and Life Sciences discusses how some of the most important scientific and societal challenges can be addressed, at least in part, by collaborative research that lies at the intersection of traditional disciplines, including biology, chemistry, and physics. This book describes how some of the mysteries of the biological world are being addressed using tools and techniques developed in the physical sciences, and identifies five areas of potentially transformative research. Work in these areas would have significant impact in both research and society at large by expanding our understanding of the physical world and by revealing new opportunities for advancing public health, technology, and stewardship of the environment. This report recommends several ways to accelerate such cross-discipline research. Many of these recommendations are directed toward those administering the faculties and resources of our great research institutions--and the stewards of our research funders, making this book an excellent resource for academic and research institutions, scientists, universities, and federal and private funding agencies.

  12. New Worlds, New Horizons in Astronomy and Astrophysics, Washington, D.C.: National Academies Press, 2010. Panel Reports New Worlds, New Horizons in Astronomy and Astrophysics, Washington, D.C.: National Academies Press, 2010. Report of the Panel on Implementing Recommendations from the New Worlds, New Horizons Decadal Survey, Washington, D.C.: National Academies Press, 2010. 2020 Vision: An Overview of New Worlds, New Horizons in Astronomy and Astrophysics, Washington, D.C.: National Academies Press, 2011

    SciTech Connect

    Shapero, Donald C.

    2012-04-05

    The NRC's Astronomy and Astrophysics decadal survey Astro2010 was organized under the umbrella of the BPA and its sister board the Space Studies Board (SSB). NASA, NSF, and DOE are the sponsors of this survey that was asked to evaluate the field of space- and ground-based astronomy and astrophysics, recommending priorities for the most important scientific and technical activities of the decade 2010-2020. The principal goals of this study were to carry out an assessment of activities in astronomy and astrophysics, including both new and previously identified concept, and to prepare a concise report that addresses the agencies supporting the field, the Congressional committees with jurisdiction over those agencies, the scientific community, and the public. Over the past 40 years, the Astronomy and Astrophysics decadal reviews have played a vital role in the selection of major astronomical activities and subsequent scientific discoveries. Some decadal survey prioritization highlights include the development of adaptive optics systems, the Very Long Baseline Array, the Hubble Space Telescope, and the Spitzer Space Telescope.

  13. California Partnership Academies: 1987-88 Evaluation Report. Policy Paper No. PP 89-9-1.

    ERIC Educational Resources Information Center

    Dayton, Charles; And Others

    During the 1987-88 school year, 12 academy programs were operating in California under state sponsorship, including the two original Peninsula Academies in operation since 1981, and 10 replications of these, called Partnership Academies. The academies are designed to reduce the number of dropouts among at-risk high school youth. They combine a…

  14. The Impact of Career Academy Programs on Student Achievement in a New Jersey Urban High School

    ERIC Educational Resources Information Center

    Ahmad, Abdul-Azeem

    2009-01-01

    The Talent Development High School (TDHS) reform model, with career academy programs, was introduced at Randolph High School. Three academies were implemented, one called the Arts and Humanities Academy (A&H) focused on careers in creative arts, law, and public service; another titled the Business and Industrial Technology Academy (BAIT) was…

  15. Career Academies: Educating Urban Students for Career Success. ERIC/CUE Digest, Number 84.

    ERIC Educational Resources Information Center

    Burnett, Gary

    This ERIC Digest reviews the school restructuring tool of career academies serving the non-college bound student. The career academy movement began with the Electrical Academy in Philadelphia, Pennsylvania. The model was exported to California where it became the basis for the Peninsula Academies in the Sequoia Union High School District and from…

  16. 78 FR 54634 - U.S. Air Force Academy Board of Visitors Notice of Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-05

    ... Department of the Air Force U.S. Air Force Academy Board of Visitors Notice of Meeting AGENCY: U.S. Air Force Academy Board of Visitors, DOD. SUMMARY: In accordance with 10 U.S.C. 9355, the U.S. Air Force Academy (USAFA) Board of Visitors (BoV) will hold a meeting in Harmon Hall, United States Air Force Academy,...

  17. Chain Effects: The Impact of Academy Chains on Low Income Students

    ERIC Educational Resources Information Center

    Hutchings, Merryn; Francis, Becky; De Vries, Robert

    2014-01-01

    The authors analysed school performance data to review how well disadvantaged pupils achieve in academy chains. They included chains only if they had at least three academies in 2013, and two sponsored secondary academies for the whole period from September 2010 to July 2013. This means that academies are included in our analysis only when there…

  18. The Prussian Academy of Sciences and Humanities during the Weimar Republic

    ERIC Educational Resources Information Center

    Hardtwig, Wolfgang

    2004-01-01

    The German Revolution of November 1918 dramatically altered the Academy's view of its relationship with government. In particular, the Academy's Prussian tradition had to be rethought. From initial wariness to grudging acceptance, the Academy came to accept the Weimar regime. This paper studies the politics of the Academy, uncovers factions and…

  19. 77 FR 21576 - Board of Visitors for the National Fire Academy

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-10

    ... SECURITY Federal Emergency Management Agency Board of Visitors for the National Fire Academy AGENCY... Committee Teleconference Meeting. SUMMARY: The Board of Visitors for the National Fire Academy (Board) will... The purpose of the Board is to review annually the programs of the National Fire Academy (Academy)...

  20. 77 FR 41196 - Board of Visitors for the National Fire Academy

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-12

    ... SECURITY Federal Emergency Management Agency Board of Visitors for the National Fire Academy AGENCY... Committee Teleconference Meeting. SUMMARY: The Board of Visitors for the National Fire Academy (Board) will... programs of the National Fire Academy (Academy) and advise the Administrator of the Federal...

  1. 77 FR 57102 - Board of Visitors for the National Fire Academy

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-17

    ... SECURITY Federal Emergency Management Agency Board of Visitors for the National Fire Academy AGENCY... Committee Meeting. SUMMARY: The Board of Visitors for the National Fire Academy (Board) will meet on October... programs of the National Fire Academy (Academy) and advise the Administrator of the Federal...

  2. The Soviet Academy of Sciences and Technological Development

    DTIC Science & Technology

    1980-12-01

    institutions of the Academy system falling into the first group are designated " Big Science," those of the second group, "Little Science." These designations...Some Big Science institutes may be limited to work of local significance. The RSFSR, represented by the Academy of Sciences, USSR, forms the main...com- ponent of Big Science. However, the regional affiliates of the USSR Soviet Outlays on R&D in 1960 and 1968, The Rand Corporation, R-1207- DDRE

  3. Service Academy 2005 Sexual Harassment and Assault Survey

    DTIC Science & Technology

    2005-12-30

    reporting and investigation; (3) eliminating sexual harassment and negative attitudes toward women at the Academy; and (4) ensuring cadet orientation ...cases, provide updates to commanders, and assist commanders in meeting annual and newcomer orientation on sexual assault training requirements. The...30-12-2005 Final Report April 2005 - December 2005 4. TITLE AND SUBTITLE 5a. CONTRACT NUMBER Service Academy 2005 Sexual Harassment and Assault Survey

  4. 2014 Service Academy Gender Relations Survey: Overview Report

    DTIC Science & Technology

    2015-02-09

    result in new relationships with people less well known. In combination, the social environment changes 678 in sophomore year that may play a role ...the 2014 Service Academy Gender Relations Survey (2014 SAGR) conducted bythe RSSC within DMD(J This survey is the seventh ofaseries ofsurveys mandated...Z39.18 2014 Service Academy Gender Relations Survey Overview Report Additional copies of this report may be obtained from: Defense Technical

  5. United States Air Force Academy (USAFA) Vertical Axis Wind Turbine.

    DTIC Science & Technology

    1980-09-01

    from the Savonius starting turbine alone. -63- SECTION VII CONCLUSIONS AND RECOMIENDATIONS 1. CONCLUSIONS One objective of the USAF Academy Wind ...Rotors, SAND76-0131. Albuquerque: July 1977. 10. Oliver, R.C. and P.R. Nixon. "Design Procedure for Coupling Savonius and Darrieus Wind Turbines ", Air...CHART -4 ESL-TR-8048 UNITED STATES AIR FORCE ACADEMY (USAFAI VERTICAL AXIS WIND TURBINE THOMAS E. KULLGREN DENNIS W. WIEDEMEIER DEPARTMENT OF ENGINEERING

  6. Neurotrophic peptides, ADNF-9 and NAP, prevent alcohol-induced apoptosis at midgestation in fetal brains of C57BL/6 mouse.

    PubMed

    Sari, Youssef; Weedman, Jason M; Nkrumah-Abrokwah, Maxwell

    2013-01-01

    Prenatal alcohol exposure is known to induce fetal brain growth deficits at different embryonic stages. We focused this study on investigating the neuroprotective effects against alcohol-induced apoptosis at midgestation using activity-dependent neurotrophic factor (ADNF)-9, a peptide (SALLRSIPA) derived from activity-dependent neurotrophic factor, and NAP, a peptide (NAPVSIPQ) derived from activity-dependent neuroprotective protein. We used an established fetal alcohol exposure mouse model. On embryonic day 7 (E7), weight-matched pregnant females were assigned to the following groups: (1) ethanol liquid diet (ALC) group with 25 % (4.49 %, v/v) ethanol-derived calories, (2) pair-fed (PF) control group, (3) ALC combined with i.p. injections (1.5 mg/kg) of ADNF-9 (ALC/ADNF-9) group, (4) ALC combined with i.p. injections (1.5 mg/kg) of NAP (ALC/NAP) group, (5) PF liquid diet combined with i.p. injections of ADNF-9 (PF/ADNF-9) group, and (6) PF liquid diet combined with i.p. injections of NAP (PF/NAP) group. On day 15 (E15), fetal brains were collected, weighed, and assayed for TdT-mediated dUTP nick end labeling (TUNEL) staining. ADNF-9 or NAP was administered daily from E7 to E15 alongside PF or ALC liquid diet exposure. Our results show that NAP and ADNF-9 significantly prevented alcohol-induced weight reduction of fetal brains. Apoptosis was determined by TUNEL staining; NAP or ADNF-9 administration alongside alcohol exposure significantly prevented alcohol-induced increase in TUNEL-positive cells in primordium of the cingulate cortex and ganglionic eminence. These findings may pave the path toward potential therapeutics against alcohol intoxication during pregnancy stages.

  7. The histone chaperones Nap1 and Vps75 bind histones H3 and H4 in a tetrameric conformation.

    PubMed

    Bowman, Andrew; Ward, Richard; Wiechens, Nicola; Singh, Vijender; El-Mkami, Hassane; Norman, David George; Owen-Hughes, Tom

    2011-02-18

    Histone chaperones physically interact with histones to direct proper assembly and disassembly of nucleosomes regulating diverse nuclear processes such as DNA replication, promoter remodeling, transcription elongation, DNA damage, and histone variant exchange. Currently, the best-characterized chaperone-histone interaction is that between the ubiquitous chaperone Asf1 and a dimer of H3 and H4. Nucleosome assembly proteins (Nap proteins) represent a distinct class of histone chaperone. Using pulsed electron double resonance (PELDOR) measurements and protein crosslinking, we show that two members of this class, Nap1 and Vps75, bind histones in the tetrameric conformation also observed when they are sequestered within the nucleosome. Furthermore, H3 and H4 trapped in their tetrameric state can be used as substrates in nucleosome assembly and chaperone-mediated lysine acetylation. This alternate mode of histone interaction provides a potential means of maintaining the integrity of the histone tetramer during cycles of nucleosome reassembly.

  8. C-Nap1 mutation affects centriole cohesion and is associated with a Seckel-like syndrome in cattle

    PubMed Central

    Floriot, Sandrine; Vesque, Christine; Rodriguez, Sabrina; Bourgain-Guglielmetti, Florence; Karaiskou, Anthi; Gautier, Mathieu; Duchesne, Amandine; Barbey, Sarah; Fritz, Sébastien; Vasilescu, Alexandre; Bertaud, Maud; Moudjou, Mohammed; Halliez, Sophie; Cormier-Daire, Valérie; E.L. Hokayem, Joyce; Nigg, Erich A.; Manciaux, Luc; Guatteo, Raphaël; Cesbron, Nora; Toutirais, Geraldine; Eggen, André; Schneider-Maunoury, Sylvie; Boichard, Didier; Sobczak-Thépot, Joelle; Schibler, Laurent

    2015-01-01

    Caprine-like Generalized Hypoplasia Syndrome (SHGC) is an autosomal-recessive disorder in Montbéliarde cattle. Affected animals present a wide range of clinical features that include the following: delayed development with low birth weight, hind limb muscular hypoplasia, caprine-like thin head and partial coat depigmentation. Here we show that SHGC is caused by a truncating mutation in the CEP250 gene that encodes the centrosomal protein C-Nap1. This mutation results in centrosome splitting, which neither affects centriole ultrastructure and duplication in dividing cells nor centriole function in cilium assembly and mitotic spindle organization. Loss of C-Nap1-mediated centriole cohesion leads to an altered cell migration phenotype. This discovery extends the range of loci that constitute the spectrum of autosomal primary recessive microcephaly (MCPH) and Seckel-like syndromes. PMID:25902731

  9. Growth of Campylobacter jejuni on nitrate and nitrite: electron transport to NapA and NrfA via NrfH and distinct roles for NrfA and the globin Cgb in protection against nitrosative stress.

    PubMed

    Pittman, Marc S; Elvers, Karen T; Lee, Lucy; Jones, Michael A; Poole, Robert K; Park, Simon F; Kelly, David J

    2007-01-01

    Pathways of electron transport to periplasmic nitrate (NapA) and nitrite (NrfA) reductases have been investigated in Campylobacter jejuni, a microaerophilic food-borne pathogen. The nap operon is unusual in lacking napC (encoding a tetra-haem c-type cytochrome) and napF, but contains a novel gene of unknown function, napL. The iron-sulphur protein NapG has a major role in electron transfer to the NapAB complex, but we show that slow nitrate-dependent growth of a napG mutant can be sustained by electron transfer from NrfH, the electron donor to the nitrite reductase NrfA. A napL mutant possessed approximately 50% lower NapA activity than the wild type but showed normal growth with nitrate as the electron acceptor. NrfA was constitutive and was shown to play a role in protection against nitrosative stress in addition to the previously identified NO-inducible single domain globin, Cgb. However, nitrite also induced cgb expression in an NssR-dependent manner, suggesting that growth of C. jejuni with nitrite causes nitrosative stress. This was confirmed by lack of growth of cgb and nssR mutants, and slow growth of the nrfA mutant, in media containing nitrite. Thus, NrfA and Cgb together provide C. jejuni with constitutive and inducible components of a robust defence against nitrosative stress.

  10. Apollo 13 Facts [Post Flight Press Conference

    NASA Technical Reports Server (NTRS)

    2001-01-01

    The Apollo 13 astronauts, James Lovell, Jr., John Swigert, Jr., and Fred Haise, Jr., are seen during this post flight press conference. They describe their mission and answer questions from the audience.

  11. Thermal Conductances of Pressed Copper Contacts

    NASA Technical Reports Server (NTRS)

    Salerno, L.; Kittel, P.; Spivak, A.

    1986-01-01

    Report describes investigation of thermal conductivities of smooth copper contacts pressed together at liquid-helium temperatures. Investigation prompted by need for accurate thermal models for infrared detectors and other cryogenic instruments.

  12. Gas-driven filter pressing in magmas

    USGS Publications Warehouse

    Sisson, T.W.; Bacon, C.R.

    1999-01-01

    Most silicic and some mafic magmas expand via second boiling if they crystallize at depths of about 10 km or less. The buildup of gas pressure due to second boiling can be relieved by expulsion of melt out of the region of crystallization, and this process of gas-driven filter pressing assists the crystallization differentiation of magmas. For gas-driven filter pressing to be effective, the region of crystallization must inflate slowly relative to buildup of pressure and expulsion of melt These conditions are satisfied in undercooled magmatic inclusions and in thin sheets of primitive magma underplating cooler magma reservoirs. Gas-driven filter pressing thereby adds fractionated melt to magma bodies. Gas-driven filter pressing is probably the dominant process by which highly evolved melts segregate from crystal mush to form aplitic dikes in granitic plutons; this process could also account for the production of voluminous, crystal-poor rhyolites.

  13. Community-acquired Clostridium difficile NAP1/027-associated diarrhea in an eighteen month old child.

    PubMed

    Quesada-Gómez, Carlos; Vargas, Pablo; López-Ureña, Diana; Gamboa-Coronado, María del Mar; Rodríguez-Cavallini, Evelyn

    2012-12-01

    Clostridium difficile infection (CDI), characterized by symptoms varying from diarrhea to life-threatening colitis, is a major complication of antibiotic therapy. Studies suggested that CDI is emerging as an important cause of childhood diarrhea in community and hospital settings. This work is the first report of a documented case of community-acquired CDI by a NAP1 hypervirulent strain in an eighteen month old child from Latin America.

  14. Exploring Platelet Chemokine Antimicrobial Activity: Nuclear Magnetic Resonance Backbone Dynamics of NAP-2 and TC-1▿

    PubMed Central

    Nguyen, Leonard T.; Kwakman, Paulus H. S.; Chan, David I.; Liu, Zhihong; de Boer, Leonie; Zaat, Sebastian A. J.; Vogel, Hans J.

    2011-01-01

    The platelet chemokines neutrophil-activating peptide-2 (NAP-2) and thrombocidin-1 (TC-1) differ by only two amino acids at their carboxy-terminal ends. Nevertheless, they display a significant difference in their direct antimicrobial activities, with the longer NAP-2 being inactive and TC-1 being active. In an attempt to rationalize this difference in activity, we studied the structure and the dynamics of both proteins by nuclear magnetic resonance (NMR) spectroscopy. Using 15N isotope-labeled protein, we confirmed that the two monomeric proteins essentially have the same overall structure in aqueous solution. However, NMR relaxation measurements provided evidence that the negatively charged carboxy-terminal residues of NAP-2 experience a restricted motion, whereas the carboxy-terminal end of TC-1 moves in an unrestricted manner. The same behavior was also seen in molecular dynamic simulations of both proteins. Detailed analysis of the protein motions through model-free analysis, as well as a determination of their overall correlation times, provided evidence for the existence of a monomer-dimer equilibrium in solution, which seemed to be more prevalent for TC-1. This finding was supported by diffusion NMR experiments. Dimerization generates a larger cationic surface area that would increase the antimicrobial activities of these chemokines. Moreover, these data also show that the negatively charged carboxy-terminal end of NAP-2 (which is absent in TC-1) folds back over part of the positively charged helical region of the protein and, in doing so, interferes with the direct antimicrobial activity. PMID:21321145

  15. Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible GAL Locus

    PubMed Central

    Chen, Xu; D'Arcy, Sheena; Radebaugh, Catherine A.; Krzizike, Daniel D.; Giebler, Holli A.; Huang, Liangquan; Nyborg, Jennifer K.; Luger, Karolin

    2016-01-01

    Histone chaperones, like nucleosome assembly protein 1 (Nap1), play a critical role in the maintenance of chromatin architecture. Here, we use the GAL locus in Saccharomyces cerevisiae to investigate the influence of Nap1 on chromatin structure and histone dynamics during distinct transcriptional states. When the GAL locus is not expressed, cells lacking Nap1 show an accumulation of histone H2A-H2B but not histone H3-H4 at this locus. Excess H2A-H2B interacts with the linker DNA between nucleosomes, and the interaction is independent of the inherent DNA-binding affinity of H2A-H2B for these particular sequences as measured in vitro. When the GAL locus is transcribed, excess H2A-H2B is reversed, and levels of all chromatin-bound histones are depleted in cells lacking Nap1. We developed an in vivo system to measure histone exchange at the GAL locus and observed considerable variability in the rate of exchange across the locus in wild-type cells. We recapitulate this variability with in vitro nucleosome reconstitutions, which suggests a contribution of DNA sequence to histone dynamics. We also find that Nap1 is required for transcription-dependent H2A-H2B exchange. Altogether, these results indicate that Nap1 is essential for maintaining proper chromatin composition and modulating the exchange of H2A-H2B in vivo. PMID:26884462

  16. Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible GAL Locus.

    PubMed

    Chen, Xu; D'Arcy, Sheena; Radebaugh, Catherine A; Krzizike, Daniel D; Giebler, Holli A; Huang, Liangquan; Nyborg, Jennifer K; Luger, Karolin; Stargell, Laurie A

    2016-04-01

    Histone chaperones, like nucleosome assembly protein 1 (Nap1), play a critical role in the maintenance of chromatin architecture. Here, we use the GAL locus in Saccharomyces cerevisiae to investigate the influence of Nap1 on chromatin structure and histone dynamics during distinct transcriptional states. When the GAL locus is not expressed, cells lacking Nap1 show an accumulation of histone H2A-H2B but not histone H3-H4 at this locus. Excess H2A-H2B interacts with the linker DNA between nucleosomes, and the interaction is independent of the inherent DNA-binding affinity of H2A-H2B for these particular sequences as measured in vitro When the GAL locus is transcribed, excess H2A-H2B is reversed, and levels of all chromatin-bound histones are depleted in cells lacking Nap1. We developed an in vivo system to measure histone exchange at the GAL locus and observed considerable variability in the rate of exchange across the locus in wild-type cells. We recapitulate this variability with in vitro nucleosome reconstitutions, which suggests a contribution of DNA sequence to histone dynamics. We also find that Nap1 is required for transcription-dependent H2A-H2B exchange. Altogether, these results indicate that Nap1 is essential for maintaining proper chromatin composition and modulating the exchange of H2A-H2B in vivo.

  17. The cortisol awakening response (CAR) in 2- to 4-year-old children: effects of acute nighttime sleep restriction, wake time, and daytime napping.

    PubMed

    Gribbin, Colleen E; Watamura, Sarah Enos; Cairns, Alyssa; Harsh, John R; Lebourgeois, Monique K

    2012-05-01

    The cortisol awakening response (CAR) is presumed critically important for healthy adaptation. The current literature, however, is hampered by systematic measurement difficulties relative to awakening, especially with young children. While reports suggest the CAR is smaller in children than adults, well-controlled research in early childhood is scarce. We examined whether robust CARs exist in 2- to 4-year-old children and if sleep restriction, wake timing, and napping influence the CAR (n = 7). During a 25-day in-home protocol, researchers collected four salivary cortisol samples (0, 15, 30, 45 min post-wake) following five polysomnographic sleep recordings on nonconsecutive days after 4 hr (morning nap), 7 hr (afternoon nap), 10 hr (evening nap), 13 hr (baseline night), and 16 hr (sleep restriction night) of wakefulness (20 samples/child). The CAR was robust after nighttime sleep, diminished after sleep restriction, and smaller but distinct after morning and afternoon (not evening) naps. Cortisol remained elevated 45 min after morning and afternoon naps. .

  18. NAP prevents acute cerebral oxidative stress and protects against long-term brain injury and cognitive impairment in a model of neonatal hypoxia-ischemia.

    PubMed

    Greggio, Samuel; de Paula, Simone; de Oliveira, Iuri M; Trindade, Cristiano; Rosa, Renato M; Henriques, João A P; DaCosta, Jaderson C

    2011-10-01

    Hypoxia-ischemia (HI) is a common cause of neonatal brain damage with lifelong morbidities in which current therapies are limited. In this study, we investigated the effect of neuropeptide NAP (NAPVSIPQ) on early cerebral oxidative stress, long-term neurological function and brain injury after neonatal HI. Seven-day-old rat pups were subjected to an HI model by applying a unilateral carotid artery occlusion and systemic hypoxia. The animals were randomly assigned to groups receiving an intraperitoneal injection of NAP (3 μg/g) or vehicle immediately (0 h) and 24 h after HI. Brain DNA damage, lipid peroxidation and reduced glutathione (GSH) content were determined 24 h after the last NAP injection. Cognitive impairment was assessed on postnatal day 60 using the spatial version of the Morris water maze learning task. Next, the animals were euthanized to assess the cerebral hemispheric volume using the Cavalieri principle associated with the counting point method. We observed that NAP prevented the acute HI-induced DNA and lipid membrane damage and also recovered the GSH levels in the injured hemisphere of the HI rat pups. Further, NAP was able to prevent impairments in learning and long-term spatial memory and to significantly reduce brain damage up to 7 weeks following the neonatal HI injury. Our findings demonstrate that NAP confers potent neuroprotection from acute brain oxidative stress, long-term cognitive impairment and brain lesions induced by neonatal HI through, at least in part, the modulation of the glutathione-mediated antioxidant system.

  19. Histone chaperones ASF1 and NAP1 differentially modulate removal of active histone marks by LID-RPD3 complexes during NOTCH silencing.

    PubMed

    Moshkin, Yuri M; Kan, Tsung Wai; Goodfellow, Henry; Bezstarosti, Karel; Maeda, Robert K; Pilyugin, Maxim; Karch, Francois; Bray, Sarah J; Demmers, Jeroen A A; Verrijzer, C Peter

    2009-09-24

    Histone chaperones are involved in a variety of chromatin transactions. By a proteomics survey, we identified the interaction networks of histone chaperones ASF1, CAF1, HIRA, and NAP1. Here, we analyzed the cooperation of H3/H4 chaperone ASF1 and H2A/H2B chaperone NAP1 with two closely related silencing complexes: LAF and RLAF. NAP1 binds RPD3 and LID-associated factors (RLAF) comprising histone deacetylase RPD3, histone H3K4 demethylase LID/KDM5, SIN3A, PF1, EMSY, and MRG15. ASF1 binds LAF, a similar complex lacking RPD3. ASF1 and NAP1 link, respectively, LAF and RLAF to the DNA-binding Su(H)/Hairless complex, which targets the E(spl) NOTCH-regulated genes. ASF1 facilitates gene-selective removal of the H3K4me3 mark by LAF but has no effect on H3 deacetylation. NAP1 directs high nucleosome density near E(spl) control elements and mediates both H3 deacetylation and H3K4me3 demethylation by RLAF. We conclude that histone chaperones ASF1 and NAP1 differentially modulate local chromatin structure during gene-selective silencing.

  20. STS-104 Pre-Launch Press Conference

    NASA Technical Reports Server (NTRS)

    2001-01-01

    George Diller, NASA Public Affairs, introduces Jim Halsell, Shuttle Program Launch Integration Manager, Dave King, NASA Director of Shuttle Processing, Michael Hawes, Deputy Associate Administrator for ISS, and John Weems, Launch Weather Officer, in this STS-104 press conference. An overview is given of the launch and mission activities, International Space Station activities during the mission, and the weather forecast for the launch. The men then answer questions from the press.

  1. STS-105 Pre-Launch Press Conference

    NASA Technical Reports Server (NTRS)

    2001-01-01

    George Diller, NASA Public Affairs, introduces Bill Gerstenmaier, Deputy Manager of the ISS Program, Dave King, NASA Director of Shuttle Processing, and Judy Konecky, Staff Meteorologist, in this STS-105 press conference. An overview is given of the success of the Expedition 2 crew, the expectations of the Expedition 3 crew, the launch countdown status, and the weather forecast for the Shuttle launch. They then answer questions from the press.

  2. STS-121: Discovery Post Landing Press Conference

    NASA Technical Reports Server (NTRS)

    2006-01-01

    On July 17, 2006 Dean Acosta (NASA Press Secretary), Mike Griffin (Administrator), Bill Gerstenmaier (Associate Administrator of Space Operations), and Mike Leinbach (NASA Launch Director) expressed how proud they were to be a part of the STS-121/ Discovery team. They also explained how flawlessly the mission performed and how it was the best mission ever flown. They proceeded to answer numerous questions from the press.

  3. Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1.

    PubMed

    Machida, Shinichi; Takaku, Motoki; Ikura, Masae; Sun, Jiying; Suzuki, Hidekazu; Kobayashi, Wataru; Kinomura, Aiko; Osakabe, Akihisa; Tachiwana, Hiroaki; Horikoshi, Yasunori; Fukuto, Atsuhiko; Matsuda, Ryo; Ura, Kiyoe; Tashiro, Satoshi; Ikura, Tsuyoshi; Kurumizaka, Hitoshi

    2014-05-06

    Homologous recombination plays essential roles in mitotic DNA double strand break (DSB) repair and meiotic genetic recombination. In eukaryotes, RAD51 promotes the central homologous-pairing step during homologous recombination, but is not sufficient to overcome the reaction barrier imposed by nucleosomes. RAD54, a member of the ATP-dependent nucleosome remodeling factor family, is required to promote the RAD51-mediated homologous pairing in nucleosomal DNA. In higher eukaryotes, most nucleosomes form higher-ordered chromatin containing the linker histone H1. However, the mechanism by which RAD51/RAD54-mediated homologous pairing occurs in higher-ordered chromatin has not been elucidated. In this study, we found that a histone chaperone, Nap1, accumulates on DSB sites in human cells, and DSB repair is substantially decreased in Nap1-knockdown cells. We determined that Nap1 binds to RAD54, enhances the RAD54-mediated nucleosome remodeling by evicting histone H1, and eventually stimulates the RAD51-mediated homologous pairing in higher-ordered chromatin containing histone H1.

  4. NapB in excess inhibits growth of Shewanella oneidensis by dissipating electrons of the quinol pool

    PubMed Central

    Jin, Miao; Zhang, Qianyun; Sun, Yijuan; Gao, Haichun

    2016-01-01

    Shewanella, a group of ubiquitous bacteria renowned for respiratory versatility, thrive in environments where various electron acceptors (EAs) of different chemical and physiological characteristics coexist. Despite being extensively studied, we still know surprisingly little about strategies by which multiple EAs and their interaction define ecophysiology of these bacteria. Previously, we showed that nitrite inhibits growth of the genus representative Shewanella oneidensis on fumarate and presumably some other CymA (quinol dehydrogenase)-dependent EAs by reducing cAMP production, which in turn leads to lowered expression of nitrite and fumarate reductases. In this study, we demonstrated that inhibition of fumarate growth by nitrite is also attributable to overproduction of NapB, the cytochrome c subunit of nitrate reductase. Further investigations revealed that excessive NapB per se inhibits growth on all EAs tested, including oxygen. When overproduced, NapB acts as an electron shuttle to dissipate electrons of the quinol pool, likely to extracellullar EAs, because the Mtr system, the major electron transport pathway for extracellular electron transport, is implicated. The study not only sheds light on mechanisms by which certain EAs, especially toxic ones, impact the bacterial ecophysiology, but also provides new insights into how electron shuttle c-type cytochromes regulate multi-branched respiratory networks. PMID:27857202

  5. Nanoconjugated NAP as a Potent and Periphery Selective Mu Opioid Receptor Modulator To Treat Opioid-Induced Constipation.

    PubMed

    Xu, Guoyan G; Zolotarskaya, Olga Yu; Williams, Dwight A; Yuan, Yunyun; Selley, Dana E; Dewey, William L; Akbarali, Hamid I; Yang, Hu; Zhang, Yan

    2017-01-12

    Opioids are the mainstay for cancer and noncancer pain management. However, their use is often associated with multiple adverse effects. Among them, the most common and persistent one is probably opioid-induced constipation (OIC). Periphery selective opioid antagonists may alleviate the symptoms of OIC without compromising the analgesic effects of opioids. Recently our laboratories have identified one novel lead compound, 17-cyclopropylmethyl-3,14β-dihydroxy-4,5α-epoxy-6β-[(4'-pyridyl)acetamido]morphinan (NAP), as a peripherally selective mu opioid receptor ligand carrying subnanomolar affinity to the mu opioid receptor and over 100-folds of selectivity over both the delta and kappa opioid receptors, with reasonable oral availability and half-life, and potential to treat OIC. Nanoparticle-based drug delivery systems are now widely considered due to their technological advantages such as good stability, high carrier capacity, low therapeutic side effects, etc. Herein we report nanoparticle supported NAP as a potential candidate for OIC treatment with improved peripheral selectivity over the original lead compound NAP.

  6. Effects of unconditioned stimulus intensity and fear extinction on subsequent sleep architecture in an afternoon nap.

    PubMed

    Sturm, Anna; Czisch, Michael; Spoormaker, Victor I

    2013-12-01

    Impaired fear extinction and disturbed sleep coincide in post-traumatic stress disorder (PTSD), but the nature of this relationship is unclear. Rapid eye movement (REM) sleep deprivation impairs fear extinction recall in rodents and young healthy subjects, and animal models have demonstrated both disrupted sleep after fear conditioning and normalized sleep after extinction learning. As a correlation between unconditioned stimulus (US) responding and subsequent sleep architecture has been observed in healthy subjects, the goal of this study was to test whether US intensity would causally affect subsequent sleep. Twenty-four young healthy subjects underwent a fear conditioning session with skin conductance response measurements before an afternoon session of polysomnographically recorded sleep (up to 120 min) in the sleep laboratory. Two factors were manipulated experimentally in a 2 × 2 design: US (electrical shock) was set at high or low intensity, and subjects did or did not receive an extinction session after fear conditioning. We observed that neither factor affected REM sleep amount, that high US intensity nominally increased sleep fragmentation (more Stage 1 sleep, stage shifts and wake after sleep onset), and that extinction increased Stage 4 amount. Moreover, reduced Stage 1 and increased Stage 4 and REM sleep were associated with subjective sleep quality of the afternoon nap. These results provide evidence for the notion that US intensity and extinction affect subsequent sleep architecture in young healthy subjects, which may provide a translational bridge from findings in animal studies to correlations observed in PTSD patients.

  7. A NAP-Family Histone Chaperone Functions in Abiotic Stress Response and Adaptation1[OPEN

    PubMed Central

    Pareek, Ashwani; Singla-Pareek, Sneh Lata

    2016-01-01

    Modulation of gene expression is one of the most significant molecular mechanisms of abiotic stress response in plants. Via altering DNA accessibility, histone chaperones affect the transcriptional competence of genomic loci. However, in contrast to other factors affecting chromatin dynamics, the role of plant histone chaperones in abiotic stress response and adaptation remains elusive. Here, we studied the physiological function of a stress-responsive putative rice (Oryza sativa) histone chaperone of the NAP superfamily: OsNAPL6. We show that OsNAPL6 is a nuclear-localized H3/H4 histone chaperone capable of assembling a nucleosome-like structure. Utilizing overexpression and knockdown approaches, we found a positive correlation between OsNAPL6 expression levels and adaptation to multiple abiotic stresses. Results of comparative transcriptome profiling and promoter-recruitment studies indicate that OsNAPL6 functions during stress response via modulation of expression of various genes involved in diverse functions. For instance, we show that OsNAPL6 is recruited to OsRad51 promoter, activating its expression and leading to more efficient DNA repair and abrogation of programmed cell death under salinity and genotoxic stress conditions. These results suggest that the histone chaperone OsNAPL6 may serve a regulatory role in abiotic stress physiology possibly via modulating nucleosome dynamics at various stress-associated genomic loci. Taken together, our findings establish a hitherto unknown link between histone chaperones and abiotic stress response in plants. PMID:27342307

  8. Directional handling qualities requirements for nap-of-the-earth tasks

    NASA Technical Reports Server (NTRS)

    Bivens, C. C.

    1986-01-01

    A piloted similator experiment designed to investigate directional axis handling qualities requirements for low-speed and hover tasks conducted by a scout/attack helicopter is described. Included were the directional characteristics of various candidate light helicopter family (LHX) configurations. The test also focused on conventional single main/tail rotor configurations where the first-order effects that contribute to the loss of tail rotor control experienced by the OH-58 series aircraft were modeled. Two types of yaw stability and control augmentation systems were implemented: washed-out yaw rate feedback and shaped control input, and a yaw rate command, heading-hold system. The results of the experiment indicate that rotorcraft configurations with high directional gust sensitivity require more yaw damping to maintain satisfactory handling qualities during nap-of-the-earth (NOE) flying tasks. It was also determined that both yaw damping and control response are critical handling qualities parameters in performing the air-to-air target acquisition and tracking task. The lack of substantial yaw damping and larger values of gust sensitivity increased the possibility of loss of directional control at low airspeeds for the tail rotor configurations.

  9. High-precision calculation of loosely bound states of LiPs+ and NaPs+

    NASA Astrophysics Data System (ADS)

    Yamashita, Takuma; Kino, Yasushi

    2015-06-01

    A positronic alkali atom would be the first step to investigate behavior of a positronium(Ps) in an external field from atoms/molecules because the system can be regarded as a simple three-body system using model potentials reflecting electron orbitals of the ion core. In order to precisely determine binding energies and structures of positronic alkali atoms (LiPs+ and NaPs+), we improve the model potential so as to reproduce highly excited atomic energy levels of alkali atoms (Li and Na). The polarization potential included by the model potential is expanded in terms of Gaussian functions to finely determine a short range part of the potential which has been assumed to be a simple form. We find better reproducibility not only of atomic levels of the alkali atoms but also of the dipole polarizability of the core ion than previous works. We construct a model potential between a positron and an ion core based on the model potential between the valence electron and ion core. Binding energies associated with a dissociation of the alkali ion core and positronium, and interparticle distances are recalculated. Our results show slightly deeper bound than other previous studies.

  10. Visual field information in Nap-of-the-Earth flight by teleoperated Helmet-Mounted displays

    NASA Technical Reports Server (NTRS)

    Grunwald, Arthur J.; Kohn, S.; Merhav, S. J.

    1991-01-01

    The human ability to derive Control-Oriented Visual Field Information from teleoperated Helmet-Mounted displays in Nap-of-the-Earth flight, is investigated. The visual field with these types of displays originates from a Forward Looking Infrared Radiation Camera, gimbal-mounted at the front of the aircraft and slaved to the pilot's line-of-sight, to obtain wide-angle visual coverage. Although these displays are proved to be effective in Apache and Cobra helicopter night operations, they demand very high pilot proficiency and work load. Experimental work presented in the paper has shown that part of the difficulties encountered in vehicular control by means of these displays can be attributed to the narrow viewing aperture and head/camera slaving system phase lags. Both these shortcomings will impair visuo-vestibular coordination, when voluntary head rotation is present. This might result in errors in estimating the Control-Oriented Visual Field Information vital in vehicular control, such as the vehicle yaw rate or the anticipated flight path, or might even lead to visuo-vestibular conflicts (motion sickness). Since, under these conditions, the pilot will tend to minimize head rotation, the full wide-angle coverage of the Helmet-Mounted Display, provided by the line-of-sight slaving system, is not always fully utilized.

  11. Systemic Mentoring for Competitiveness: The Model of the Timbuktu Academy

    NASA Astrophysics Data System (ADS)

    Bagayoko, Diola

    2010-02-01

    The Timbuktu Academy is a comprehensive, systemic mentoring program at Southern University and A&M College in Baton Rouge (SUBR), Louisiana. We define systemic mentoring as one that is woven into the core functions of an organization. For most universities, those functions include instruction, research, and service. While the Academy has programs for pre-college and graduate students, its Ten-Strand Systemic Mentoring Model was specifically tailored to undergraduate education. We discuss basic considerations that led to the paradigm, programs, activities, and results of the Timbuktu Academy. The proper implementation of the Ten-Strand Systemic Mentoring Model couples teaching and superior learning, on the one hand, and integrates research and education, on the other hand. For undergraduate education, key strands include support (financially or otherwise), scientific advisement, research participation (academic year or summer), immersion in a professional culture, monitoring, and guidance to graduate school. From the summer of 1994 to 2009, the Academy has engaged 2,093 pre-college scholars in its summer programs. To date, the Academy has assisted in the production of one hundred seventy (170) minority undergraduate scholars who have earned a Bachelor of Science degree. Seventy (70) of 83 physics graduates, twenty (20) of 29 chemistry graduates, and twenty-two (22) of 49 engineering graduates have earned graduate degrees or are successfully enrolled in graduate school, with emphasis on the pursuit of the Ph.D. For the above model and results, the Timbuktu Academy received the 2002 U.S. Presidential Award for Excellence in Science, Mathematics, and Engineering Mentoring. Its director was among the first individual recipients of this award in 1996. The handouts accompanying this presentation are intended to facilitate the adaptive replication of the Timbuktu Academy by individuals, departments, colleges and universities, and other organizations. )

  12. DOD Service Academies. Academy Preparatory Schools Need a Clearer Mission and Better Oversight. Report to the Chairmen, Senate and House Committees on Armed Services.

    ERIC Educational Resources Information Center

    General Accounting Office, Washington, DC. National Security and International Affairs Div.

    The three service academy prep schools were assessed to determine how well the schools accomplished their missions and whether they were cost effective. A review was performed at seven sites, the: Department of Defense (DOD) and service headquarters, Washington, District of Columbia; Military Academy, West Point, New York; Air Force Academy,…

  13. Seasonal changes in anthropometric and physical characteristics within English academy rugby league players.

    PubMed

    Till, Kevin; Jones, Ben; Emmonds, Stacey; Tester, Emma; Fahey, Jack; Cooke, Carlton

    2014-09-01

    Professional rugby league clubs implement training programmes for the development of anthropometric and physical characteristics within an academy programme. However, research that examines seasonal changes in these characteristics is limited. The purpose of the study was to evaluate the seasonal changes in anthropometric and physical characteristics of academy rugby league players by age category (i.e., under 14, 16, 18, 20). Data were collected on 75 players pre- and postseason over a 6-year period (resulting in a total of 195 assessments). Anthropometric (body mass, sum of 4 skinfolds) and physical (10- and 20-m sprint, vertical jump, Yo-Yo intermittent recovery test and 1 repetition maximum squat, bench press, and prone row) measures were collected. The under 14s and 16s showed greater seasonal improvements in body mass (e.g., under 14s = 7.4 ± 4.3% vs. under 20s = 1.2 ± 3.3%) and vertical jump performance than under 18s and under 20s. In contrast, under 18s and under 20s players showed greater seasonal improvements in Yo-Yo performance and 10-m sprint (e.g., under 14s = 1.3 ± 3.9% vs. under 20s = -1.9 ± 1.2%) in comparison to under 14s and under 16s. Seasonal strength improvements were greater for the under 18s compared with under 20s. This study provides comparative data for seasonal changes in anthropometric and physical characteristics within rugby league players aged 13-20 years. Coaches should be aware that seasonal improvements in speed may not exist within younger age categories, until changes in body mass stabilize and consider monitoring changes in other characteristics (e.g., momentum). Large interplayer variability suggests that player development should be considered on an individual and longitudinal basis.

  14. Designing and implementing the Army Nursing Leader Academy.

    PubMed

    Dunemn, Kathleen; Hopkins-Chadwick, Denise L; Connally, Tina; Bramley, Kelly

    2011-01-01

    In 2008, the Chief of the Army Nurse Corps directed a thorough review of existing training programs available to and provided for Army Nursing personnel for the development of full-spectrum leaders for Army Nursing. The review provided the gap analysis necessary to restructure courses provided by the Department of Nursing Science at the Army Medical Department Center and School. This new grouping of courses is referred to as the Army Nursing Leader Academy. The Army Nursing Leader Academy is the first of its kind in that it addresses career-long learning of all Army Nursing by focusing on building skills, knowledge, and behaviors to produce sustainable, full-spectrum leaders. The Nursing Leader Academy consists of a series of sequential nurse leader development courses combined with a web based resource center. Grounded in the Patient CaringTouch System, guided by nurse competencies, and gauged by the Leader Capabilities Map, the Nursing Leader Academy provides learning that is relevant and timely designed to reinforce enterprise values and culture to ensure readiness for successive roles and positions. Full implementation of the Nursing Leader Academy will include the evidence-based elements of formal schooling, coaching, self-development, functional/technical (competency attainment), and professional experiences.

  15. CosmoAcademy Training and Certification for Scientists and Engineers

    NASA Astrophysics Data System (ADS)

    Noel-Storr, Jacob; Buxner, Sanlyn; Grier, Jennifer A.; Gay, Pamela L.; CosmoQuest Team

    2016-10-01

    CosmoQuest is a virtual research facility bringing together scientists, citizens, and learners of all ages. CosmoQuest offers classes, training, and learning opportunities online through CosmoAcademy, offering opportunities for all kinds of learners to become more connected to the science of the Universe. In this poster we describe CosmoAcademy opportunities for Subject Matter Experts (SMEs), scientists and engineers who are interested in broadening their impact of their work by providing learning opportunities for those outside of the scientific community. CosmoAcademy offers SME programs at a variety of levels and across a variety of topics in formal and informal education and outreach -- ranging from sharing the results of your work on social media, through delivering an online class series, to partnering with teachers and schools. SMEs may combine sequences of training to earn certification at various levels for their participation in the CosmoAcademy programs. SMEs who have been trained may also apply to teach CosmoAcademy classes for the community on subjects of their expertise to build a rich and engaging learning resource for members of society who wish to understand more about the Universe.

  16. An American Academy for Training Safeguards Inspectors - An Idea Revisited

    SciTech Connect

    Philip Casey Durst; Robert Bean

    2010-07-01

    In 2009, we presented the idea of an American academy for training safeguards inspectors for the International Atomic Energy Agency (IAEA), due to the declining percentage of Americans in that international organization. In this paper we assert that there is still a compelling need for this academy. While the American Safeguards Academy would be useful in preparing and pre-training American inspectors for the IAEA, it would also be useful for preparing Americans for domestic safeguards duties in the U.S. Department of Energy (DOE), U.S. DOE National Laboratories, and the U.S. Nuclear Regulatory Commission (NRC). It is envisioned that such an academy would train graduate and post-graduate university students, DOE National Laboratory interns, and nuclear safeguards professionals in the modern equipment, safeguards measures, and approaches currently used by the IAEA. It is also envisioned that the Academy would involve the domestic nuclear industry, which could provide use of commercial nuclear facilities for tours and demonstrations of the safeguards tools and methods in actual nuclear facilities. This would be in support of the U.S. DOE National Nuclear Security Administration’s Next Generation Safeguards Initiative (NGSI). This training would also help American nuclear safeguards and non-proliferation professionals better understand the potential limitations of the current tools used by the IAEA and give them a foundation from which to consider even more effective and efficient safeguards measures and approaches.

  17. Disparate response to microoxia and nitrogen oxides of the Bradyrhizobium japonicum napEDABC, nirK and norCBQD denitrification genes.

    PubMed

    Bueno, Emilio; Robles, Eloy F; Torres, María J; Krell, Tino; Bedmar, Eulogio J; Delgado, María J; Mesa, Socorro

    2017-02-03

    Expression of the Bradyrhizobium japonicum napEDABC, nirK and norCBQD denitrification genes requires low oxygen (O2) tension and nitrate (NO3(-)), through a regulatory network comprised of two coordinated cascades, FixLJ-FixK2-NnrR and RegSR-NifA. To precisely understand how these signals are integrated in the FixLJ-FixK2-NnrR circuit, we analyzed β-Galactosidase activities from napE-lacZ, nirK-lacZ and norC-lacZ fusions, and performed analyses of NapC and NorC levels as well as periplasmic nitrate reductase (Nap) activity, in B. japonicum wildtype and fixK2 and nnrR mutant backgrounds. While microoxic conditions (2% O2 at headspace) were sufficient to induce expression of napEDABC and nirK genes and this control depends on FixK2, norCBQD expression requires, in addition to microoxia, nitric oxide gas (NO) and both FixK2 and NnrR transcription factors. Purified FixK2 protein directly interacted and activated transcription in collaboration with B. japonicum RNA polymerase (RNAP) from the napEDABC and nirK promoters, but not from the norCBQD promoter. Further, recombinant NnrR protein bound exclusively to the norCBQD promoter in an O2-sensitive manner. Our work suggest a disparate regulation of B. japonicum denitrifying genes expression with regard to their dependency to microoxia, nitrogen oxides (NOx), and the regulatory proteins FixK2 and NnrR. In this control, expression of napEDABC and nirK genes requires microoxic conditions and directly depends on FixK2, while expression of norCBQD genes relies on NO, being NnrR the candidate which directly interacts with the norCBQD promoter.

  18. Influence of night-shift and napping at work on urinary melatonin, 17-β-estradiol and clock gene expression in pre-menopausal nurses.

    PubMed

    Bracci, M; Copertaro, A; Manzella, N; Staffolani, S; Strafella, E; Nocchi, L; Barbaresi, M; Copertaro, B; Rapisarda, V; Valentino, M; Santarelli, L

    2013-01-01

    Night-workers experience disruption of the sleep-wake cycle and light at night which may increase breast cancer risk by suppressing the nocturnal melatonin surge, resulting in higher levels of circulating estrogens. Night-work may also deregulate peripheral clock genes which have been found to be altered in breast cancer. This study investigated urinary 6-sulfatoxymelatonin (aMT6s), serum 17-beta-estradiol levels in premenopausal shift nurses at the end of the night-shift compared to a control group of daytime nurses. Peripheral clock gene expression in lymphocytes were also investigated. All participants were sampled in the follicular phase of the menstrual cycle. The effect of nurses’ ability to take a short nap during the night-shift was also explored. The shift-work group had significantly lower aMT6s levels than daytime nurses independently of a nap. Night-shift napping significantly influences 17-beta-estradiol levels resulting in higher outcomes in nurses who do not take a nap compared to napping group and daytime workers. Peripheral clock genes expression investigated was not significantly different among the groups. Our findings suggest that shift nurses experience changes in aMT6s levels after a night-shift. Napping habits influence 17-beta-estradiol levels at the end of a night-shift. These findings might be related to the increased cancer risk reported in night-shift workers and suggest that a short nap during night-shifts may exert a positive effect.

  19. Effects of the transport site conformation on the binding of external NAP-taurine to the human erythrocyte anion exchange system: evidence for intrinsic asymmetry

    SciTech Connect

    Knauf, P.A.; Law, F.Y.; Tarshis, T.; Furuya, W.

    1984-05-01

    External N-(4-azido (NAP-taurine) inhibits human red cell chloride exchange by binding to a site that is distinct from the chloride transport site. Increases in the intracellular chloride concentration (at constant external chloride) cause an increase in the inhibitory potency of external NAP-taurine. This effect is not due to the changes in pH or membrane potential that usually accompany a chloride gradient, since even when these changes are reversed or eliminated the inhibitory potency remains high. According to the ping-pong model for anion exchange, such transmembrane effects of intracellular chloride on external NAP-taurine can be explained if NAP-taurine only binds to its site when the transport site is in the outward-facing (E/sub o/ or ECl/sub o/) form. Since NAP-taurine prevents the conformational change from ECl/sub o/ to ECl/sub i/, it must lock the system in the outward-facing form. NAP-taurine can therefore be used just like the competitive inhibitor H/sub 2/DIDS (4,4'-diisothiocyano-1,2-diphenylethane-2,2'-disulfonic acid) to monitor the fraction of transport sites that face outward. A quantitative analysis of the effects of chloride gradients on the inhibitory potency of NAP-taurine and H/sub 2/DIDS reveals that the transport system is intrinsically asymmetric, such that when Cl/sub i/ = Cl/sub o/, most of the unloaded transport sites face the cytoplasmic side of the membrane. 30 references, 7 figures, 3 tables.

  20. The role of a short post-lunch nap in improving cognitive, motor, and sprint performance in participants with partial sleep deprivation.

    PubMed

    Waterhouse, J; Atkinson, G; Edwards, B; Reilly, T

    2007-12-01

    The aim of this study was to determine the effects of a post-lunch nap on subjective alertness and performance following partial sleep loss. Ten healthy males (mean age 23.3 years, s = 3.4) either napped or sat quietly from 13:00 to 13:30 h after a night of shortened sleep (sleep 23:00-03:00 h only). Thirty minutes after the afternoon nap or control (no-nap) condition, alertness, short-term memory, intra-aural temperature, heart rate, choice reaction time, grip strength, and times for 2-m and 20-m sprints were recorded. The afternoon nap lowered heart rate and intra-aural temperature. Alertness, sleepiness, short-term memory, and accuracy at the 8-choice reaction time test were improved by napping (P < 0.05), but mean reaction times and grip strength were not affected (P > 0.05). Sprint times were improved. Mean time for the 2-m sprints fell from 1.060 s (s(x) = 0.018) to 1.019 s (s(x) = 0.019) (P = 0.031 paired t-test); mean time for the 20-m sprints fell from 3.971 s (s(x) = 0.054) to 3.878 s (s(x) = 0.047) (P = 0.013). These results indicate that a post-lunch nap improves alertness and aspects of mental and physical performance following partial sleep loss, and have implications for athletes with restricted sleep during training or before competition.