DDE residues in young wood ducks (Aix sponsa) near a former DDT manufacturing plant

USGS Publications Warehouse

Fleming, W.J.; Cromartie, E.

1981-01-01

Breast muscle DDE residues were as high as 5.8 ppm wet-weight basis and 280 ppm lipid-weight basis in young wood ducks (Aix Sponsa) collected on Wheeler National Wildlife Refuge near a former DDT manufacturing plant in northern Alabama. The average DDE residue in wood ducks collected nearest the plant was 46 times background levels 74 km from the plant.

Measuring dry plant residues in grasslands: A case study using AVIRIS

NASA Technical Reports Server (NTRS)

Fitzgerald, Michael; Ustin, Susan L.

1992-01-01

Grasslands, savannah, and hardwood rangelands are critical ecosystems and sensitive to disturbance. Approximately 20 percent of the Earth's surface are grasslands and represent 3 million ha. in California alone. Developing a methodology for estimating disturbance and the effects of cumulative impacts on grasslands and rangelands is needed to effectively monitor these ecosystems. Estimating the dry biomass residue remaining on rangelands at the end of the growing season provides a basis for evaluating the effectiveness of land management practices. The residual biomass is indicative of the grazing pressure and provides a measure of the system capacity for nutrient cycling since it represents the maximum organic matter available for decomposition, and finally, provides a measure of the erosion potential for the ecosystem. Remote sensing presents a possible method for measuring dry residue. However, current satellites have had limited application due to the coarse spatial scales (relative to the patch dynamics) and insensitivity of the spectral coverage to resolve dry plant material. Several hypotheses for measuring the biochemical constituents of dry plant material, particularly cellulose and lignin, using high spectral resolution sensors were proposed. The use of Airborne Visible/Infrared Imaging Spectrometers (AVIRIS) to measure dry plant residues over an oak savannah on the eastern slopes of the Coast Range in central California was investigated and it was asked what spatial and spectral resolutions are needed to quantitatively measure dry plant biomass in this ecosystem.

The effect of sludge water treatment plant residuals on the properties of compressed brick

NASA Astrophysics Data System (ADS)

Shamsudin, Shamrul-Mar; Shahidan, S.; Azmi, M. A. M.; Ghaffar, S. A.; Ghani, M. B. Abdul; Saiful Bahari, N. A. A.; Zuki, S. S. M.

2017-11-01

The focus of this study is on the production of compressed bricks which contains sludge water treatment plant (SWTP) residuals obtained from SAJ. The main objective of this study is to utilise and incorporate discarded material (SWTP) in the form of residual solution to produce compressed bricks. This serves as one of the recycling efforts to conserve the environment. This study determined the optimum mix based on a mix ratio of 1:2:4 (cement: sand: soil) in the production of compressed bricks where 5 different mixes were investigated i. e. 0%, 5%, 10%, 20%, and 30% of water treatment plant residue solution. The production of the compressed bricks is in accordance with the Malaysian Standard MS 7.6: 1972 and British Standard BS 3921: 1985 - Compressive Strength & Water Absorption. After being moulded and air dried, the cured bricks were subjected to compression tests and water absorption tests. Based on the tests conducted, it was found that 20% of water treatment plant residue solution which is equivalent to 50% of soil content replacement with a mix composition of [10: cement] [20: sand] [20: soil] [20: water treatment plant residue solution] is the optimum mix. It was also observed that the bricks containing SWTP residuals were lighter in weight compared to the control specimens

Nanometer-scale structure of alkali-soluble bio-macromolecules of maize plant residues explains their recalcitrance in soil.

PubMed

Adani, Fabrizio; Salati, Silvia; Spagnol, Manuela; Tambone, Fulvia; Genevini, Pierluigi; Pilu, Roberto; Nierop, Klaas G J

2009-07-01

The quantity and quality of plant litter in the soil play an important role in the soil organic matter balance. Besides other pedo-climatic aspects, the content of recalcitrant molecules of plant residues and their chemical composition play a major role in the preservation of plant residues. In this study, we report that intrinsically resistant alkali-soluble bio-macromolecules extracted from maize plant (plant-humic acid) (plant-HA) contribute directly to the soil organic matter (OM) by its addition and conservation in the soil. Furthermore, we also observed that a high syringyl/guaiacyl (S/G) ratio in the lignin residues comprising the plant tissue, which modifies the microscopic structure of the alkali-soluble plant biopolymers, enhances their recalcitrance because of lower accessibility of molecules to degrading enzymes. These results are in agreement with a recent study, which showed that the humic substance of soil consists of a mixture of identifiable biopolymers obtained directly from plant tissues that are added annually by maize plant residues.

Properties of Soil Pore Space Regulate Pathways of Plant Residue Decomposition and Community Structure of Associated Bacteria

PubMed Central

Negassa, Wakene C.; Guber, Andrey K.; Kravchenko, Alexandra N.; Marsh, Terence L.; Hildebrandt, Britton; Rivers, Mark L.

2015-01-01

Physical protection of soil carbon (C) is one of the important components of C storage. However, its exact mechanisms are still not sufficiently lucid. The goal of this study was to explore the influence of soil structure, that is, soil pore spatial arrangements, with and without presence of plant residue on (i) decomposition of added plant residue, (ii) CO2 emission from soil, and (iii) structure of soil bacterial communities. The study consisted of several soil incubation experiments with samples of contrasting pore characteristics with/without plant residue, accompanied by X-ray micro-tomographic analyses of soil pores and by microbial community analysis of amplified 16S–18S rRNA genes via pyrosequencing. We observed that in the samples with substantial presence of air-filled well-connected large (>30 µm) pores, 75–80% of the added plant residue was decomposed, cumulative CO2 emission constituted 1,200 µm C g-1 soil, and movement of C from decomposing plant residue into adjacent soil was insignificant. In the samples with greater abundance of water-filled small pores, 60% of the added plant residue was decomposed, cumulative CO2 emission constituted 2,000 µm C g-1 soil, and the movement of residue C into adjacent soil was substantial. In the absence of plant residue the influence of pore characteristics on CO2 emission, that is on decomposition of the native soil organic C, was negligible. The microbial communities on the plant residue in the samples with large pores had more microbial groups known to be cellulose decomposers, that is, Bacteroidetes, Proteobacteria, Actinobacteria, and Firmicutes, while a number of oligotrophic Acidobacteria groups were more abundant on the plant residue from the samples with small pores. This study provides the first experimental evidence that characteristics of soil pores and their air/water flow status determine the phylogenetic composition of the local microbial community and directions and magnitudes of soil C

Properties of soil pore space regulate pathways of plant residue decomposition and community structure of associated bacteria

DOE PAGES

Negassa, Wakene C.; Guber, Andrey K.; Kravchenko, Alexandra N.; ...

2015-07-01

Physical protection of soil carbon (C) is one of the important components of C storage. However, its exact mechanisms are still not sufficiently lucid. The goal of this study was to explore the influence of soil structure, that is, soil pore spatial arrangements, with and without presence of plant residue on (i) decomposition of added plant residue, (ii) CO₂ emission from soil, and (iii) structure of soil bacterial communities. The study consisted of several soil incubation experiments with samples of contrasting pore characteristics with/without plant residue, accompanied by X-ray micro-tomographic analyses of soil pores and by microbial community analysis ofmore » amplified 16S–18S rRNA genes via pyrosequencing. We observed that in the samples with substantial presence of air-filled well-connected large (>30 µm) pores, 75–80% of the added plant residue was decomposed, cumulative CO₂ emission constituted 1,200 µm C g⁻¹ soil, and movement of C from decomposing plant residue into adjacent soil was insignificant. In the samples with greater abundance of water-filled small pores, 60% of the added plant residue was decomposed, cumulative CO₂ emission constituted 2,000 µm C g⁻¹ soil, and the movement of residue C into adjacent soil was substantial. In the absence of plant residue the influence of pore characteristics on CO₂ emission, that is on decomposition of the native soil organic C, was negligible. The microbial communities on the plant residue in the samples with large pores had more microbial groups known to be cellulose decomposers, that is, Bacteroidetes, Proteobacteria, Actinobacteria, and Firmicutes, while a number of oligotrophic Acidobacteria groups were more abundant on the plant residue from the samples with small pores. This study provides the first experimental evidence that characteristics of soil pores and their air/water flow status determine the phylogenetic composition of the local microbial community and directions and

Properties of soil pore space regulate pathways of plant residue decomposition and community structure of associated bacteria

DOE Office of Scientific and Technical Information (OSTI.GOV)

Negassa, Wakene C.; Guber, Andrey K.; Kravchenko, Alexandra N.

Physical protection of soil carbon (C) is one of the important components of C storage. However, its exact mechanisms are still not sufficiently lucid. The goal of this study was to explore the influence of soil structure, that is, soil pore spatial arrangements, with and without presence of plant residue on (i) decomposition of added plant residue, (ii) CO₂ emission from soil, and (iii) structure of soil bacterial communities. The study consisted of several soil incubation experiments with samples of contrasting pore characteristics with/without plant residue, accompanied by X-ray micro-tomographic analyses of soil pores and by microbial community analysis ofmore » amplified 16S–18S rRNA genes via pyrosequencing. We observed that in the samples with substantial presence of air-filled well-connected large (>30 µm) pores, 75–80% of the added plant residue was decomposed, cumulative CO₂ emission constituted 1,200 µm C g⁻¹ soil, and movement of C from decomposing plant residue into adjacent soil was insignificant. In the samples with greater abundance of water-filled small pores, 60% of the added plant residue was decomposed, cumulative CO₂ emission constituted 2,000 µm C g⁻¹ soil, and the movement of residue C into adjacent soil was substantial. In the absence of plant residue the influence of pore characteristics on CO₂ emission, that is on decomposition of the native soil organic C, was negligible. The microbial communities on the plant residue in the samples with large pores had more microbial groups known to be cellulose decomposers, that is, Bacteroidetes, Proteobacteria, Actinobacteria, and Firmicutes, while a number of oligotrophic Acidobacteria groups were more abundant on the plant residue from the samples with small pores. This study provides the first experimental evidence that characteristics of soil pores and their air/water flow status determine the phylogenetic composition of the local microbial community and directions and

Properties of soil pore space regulate pathways of plant residue decomposition and community structure of associated bacteria.

PubMed

Negassa, Wakene C; Guber, Andrey K; Kravchenko, Alexandra N; Marsh, Terence L; Hildebrandt, Britton; Rivers, Mark L

2015-01-01

Physical protection of soil carbon (C) is one of the important components of C storage. However, its exact mechanisms are still not sufficiently lucid. The goal of this study was to explore the influence of soil structure, that is, soil pore spatial arrangements, with and without presence of plant residue on (i) decomposition of added plant residue, (ii) CO2 emission from soil, and (iii) structure of soil bacterial communities. The study consisted of several soil incubation experiments with samples of contrasting pore characteristics with/without plant residue, accompanied by X-ray micro-tomographic analyses of soil pores and by microbial community analysis of amplified 16S-18S rRNA genes via pyrosequencing. We observed that in the samples with substantial presence of air-filled well-connected large (>30 µm) pores, 75-80% of the added plant residue was decomposed, cumulative CO2 emission constituted 1,200 µm C g(-1) soil, and movement of C from decomposing plant residue into adjacent soil was insignificant. In the samples with greater abundance of water-filled small pores, 60% of the added plant residue was decomposed, cumulative CO2 emission constituted 2,000 µm C g(-1) soil, and the movement of residue C into adjacent soil was substantial. In the absence of plant residue the influence of pore characteristics on CO2 emission, that is on decomposition of the native soil organic C, was negligible. The microbial communities on the plant residue in the samples with large pores had more microbial groups known to be cellulose decomposers, that is, Bacteroidetes, Proteobacteria, Actinobacteria, and Firmicutes, while a number of oligotrophic Acidobacteria groups were more abundant on the plant residue from the samples with small pores. This study provides the first experimental evidence that characteristics of soil pores and their air/water flow status determine the phylogenetic composition of the local microbial community and directions and magnitudes of soil C

An investigation of accelerating mode and decelerating mode constant-momentum mass spectrometry and their application to a residual gas analyzer

NASA Technical Reports Server (NTRS)

Ng, Y. S.

1977-01-01

A theoretical analysis of constant momentum mass spectrometry was made. A maximum resolving power for the decelerating mode constant momentum mass spectrometer was shown theoretically to exist for a beam of ions of known energy. A vacuum system and an electron beam ionization source was constructed. Supporting electronics for a residual gas analyzer were built. Experimental investigations of various types of accelerating and decelerating impulsive modes of a constant momentum mass spectrometer as applied to a residual gas analyzer were made. The data indicate that the resolving power for the decelerating mode is comparable to that of the accelerating mode.

Longitudinal and transverse dynamics of ions from residual gas in an electron accelerator

NASA Astrophysics Data System (ADS)

Gamelin, A.; Bruni, C.; Radevych, D.

2018-05-01

The ion cloud produced from residual gas in an electron accelerator can degrade machine performances and produce instabilities. The ion dynamics in an accelerator is governed by the beam-ion interaction, magnetic fields and eventual mitigation strategies. Due to the fact that the beam has a nonuniform transverse size along its orbit, the ions move longitudinally and accumulate naturally at some points in the accelerator. In order to design effective mitigation strategies it is necessary to understand the ion dynamics not only in the transverse plane but also in the longitudinal direction. After introducing the physics behind the beam-ion interaction, we show how to get accumulation points for a realistic electron storage ring lattice. Simulations of the ion cloud dynamics, including the effect of magnetic fields on the ions, clearing electrodes and clearing gaps are shown. Longitudinal ion trapping due to the magnetic mirror effect in the dipole fringe fields is also detailed. Finally, the effectiveness of clearing electrode using longitudinal clearing fields is discussed and compared to clearing electrodes producing transverse field only.

Light accelerates plant responses to warming.

PubMed

De Frenne, Pieter; Rodríguez-Sánchez, Francisco; De Schrijver, An; Coomes, David A; Hermy, Martin; Vangansbeke, Pieter; Verheyen, Kris

2015-08-17

Competition for light has profound effects on plant performance in virtually all terrestrial ecosystems. Nowhere is this more evident than in forests, where trees create environmental heterogeneity that shapes the dynamics of forest-floor communities(1-3). Observational evidence suggests that biotic responses to both anthropogenic global warming and nitrogen pollution may be attenuated by the shading effects of trees and shrubs(4-9). Here we show experimentally that tree shade is slowing down changes in below-canopy communities due to warming. We manipulated levels of photosynthetically active radiation, temperature and nitrogen, alone and in combination, in a temperate forest understorey over a 3-year period, and monitored the composition of the understorey community. Light addition, but not nitrogen enrichment, accelerated directional plant community responses to warming, increasing the dominance of warmth-preferring taxa over cold-tolerant plants (a process described as thermophilization(6,10-12)). Tall, competitive plants took greatest advantage of the combination of elevated temperature and light. Warming of the forest floor did not result in strong community thermophilization unless light was also increased. Our findings suggest that the maintenance of locally closed canopy conditions could reduce, at least temporarily, warming-induced changes in forest floor plant communities.

Growth and Productivity Response of Hybrid Rice to Application of Animal Manures, Plant Residues and Phosphorus

PubMed Central

Amanullah; Khan, Shams-ul-Tamraiz; Iqbal, Asif; Fahad, Shah

2016-01-01

The objective of this research was to evaluate the impact of organic sources (animal manures vs. plant residues at the rate of 10 t ha−1 each) on the productivity of hybrid rice (Oryza sativa L.) production under different levels of phosphorus (0, 30, 60, and 90 kg P ha−1) fertilization. Two separate field experiments were conducted. In experiment (1), impact of three animal manures sources (cattle, sheep, and poultry manures) and P levels were studied along with one control plot (no animal manure and P applied) was investigated. In experiment (2), three plant residues sources (peach leaves, garlic residues, and wheat straw) and P levels were studied along with one control plot (no plant residues and P applied). Both the experiments were carried out on small land farmer field at District Swabi, Khyber Pakhtunkhwa Province (Northwest Pakistan) during summer 2015. The results revealed that in both experiments the control plot had significantly (p ≤ 0.05) less productivity than the average of all treated plots with organic sources and P level. The increase in P levels in both experiments (animal manure vs. plant residues) resulted in higher rice productivity (90 > 60 > 30 > 0 kg P ha−1). In the experiment under animal manures, application of poultry manure increased rice productivity as compared with sheep and cattle manures (poultry > sheep > cattle manures). In the experiment under plant residues, application of peach leaves or garlic residues had higher rice productivity than wheat straw (peach leaves = garlic residues > wheat straw). On average, rice grown under animal manures produced about 20% higher grain yield than rice grown under crop residues. We conclude from this study that application of 90 kg P ha−1 along with combined application of animal manures, especially poultry manure increases rice productivity. Also, the use of either garlic residues or peach leaves, never applied before as organic manures, can increase crop productivity and will help

Analysis of Residual Acceleration Effects on Transport and Segregation During Directional Solidification of Tin-Bismuth in the MEPHISTO Furnace Facility

NASA Technical Reports Server (NTRS)

Alexander, J. Iwan D.; Lizee, Arnaud

1996-01-01

The object of this work, started in March of 1995, is to approach the problem of determining the transport conditions (and effects of residual acceleration) during the plane-front directional solidification of a tin-bismuth alloy under low gravity conditions. The work involves using a combination of 2- and 3-D numerical models, scaling analyses, 1-D models and the results of ground-based and low-gravity experiments. The experiments conducted in the MEPHISTO furnace facility during the USMP-3 spaceflight which took place earlier this year (22 Feb. - 6 Mar. 1996). This experiment represents an unprecedented opportunity to make a quantitative correlation between residual accelerations and the response of an actual experimental solidification system

Technical feasibility and carbon footprint of biochar co-production with tomato plant residue.

PubMed

Llorach-Massana, Pere; Lopez-Capel, Elisa; Peña, Javier; Rieradevall, Joan; Montero, Juan Ignacio; Puy, Neus

2017-09-01

World tomato production is in the increase, generating large amounts of organic agricultural waste, which are currently incinerated or composted, releasing CO 2 into the atmosphere. Organic waste is not only produced from conventional but also urban agricultural practices due recently gained popularity. An alternative to current waste management practices and carbon sequestration opportunity is the production of biochar (thermally converted biomass) from tomato plant residues and use as a soil amendment. To address the real contribution of biochar for greenhouse gas mitigation, it is necessary to assess the whole life cycle from the production of the tomato biomass feedstock to the actual distribution and utilisation of the biochar produced in a regional context. This study is the first step to determine the technical and environmental potential of producing biochar from tomato plant (Solanum lycopersicum arawak variety) waste biomass and utilisation as a soil amendment. The study includes the characterisation of tomato plant residue as biochar feedstock (cellulose, hemicellulose, lignin and metal content); feedstock thermal stability; and the carbon footprint of biochar production under urban agriculture at pilot and small-scale plant, and conventional agriculture at large-scale plant. Tomato plant residue is a potentially suitable biochar feedstock under current European Certification based on its lignin content (19.7%) and low metal concentration. Biomass conversion yields of over 40%, 50% carbon stabilization and low pyrolysis temperature conditions (350-400°C) would be required for biochar production to sequester carbon under urban pilot scale conditions; while large-scale biochar production from conventional agricultural practices have not the potential to sequestrate carbon because its logistics, which could be improved. Therefore, the diversion of tomato biomass waste residue from incineration or composting to biochar production for use as a soil amendment

A kinetic approach to evaluate salinity effects on carbon mineralization in a plant residue-amended soil*

PubMed Central

Nourbakhsh, Farshid; Sheikh-Hosseini, Ahmad R.

2006-01-01

The interaction of salinity stress and plant residue quality on C mineralization kinetics in soil is not well understood. A laboratory experiment was conducted to study the effects of salinity stress on C mineralization kinetics in a soil amended with alfalfa, wheat and corn residues. A factorial combination of two salinity levels (0.97 and 18.2 dS/m) and four levels of plant residues (control, alfalfa, wheat and corn) with three replications was performed. A first order kinetic model was used to describe the C mineralization and to calculate the potentially mineralizable C. The CO2-C evolved under non-saline condition, ranged from 814.6 to 4842.4 mg CO2-C/kg in control and alfalfa residue-amended soils, respectively. Salinization reduced the rates of CO2 evolution by 18.7%, 6.2% and 5.2% in alfalfa, wheat and corn residue-amended soils, respectively. Potentially mineralizable C (C 0) was reduced significantly in salinized alfalfa residue-treated soils whereas, no significant difference was observed for control treatments as well as wheat and corn residue-treated soils. We concluded that the response pattern of C mineralization to salinity stress depended on the plant residue quality and duration of incubation. PMID:16972320

Phosphate Removal and Recovery using Drinking Water Plant Waste Residuals

EPA Science Inventory

Water treatment plants are used to provide safe drinking water. In parallel, however, they also produce a wide variety of waste products which, in principle, could be possible candidates as resources for different applications. Calcium carbonate is one of such residual waste in ...

Effect of crop residues on soil properties, plant growth, and crop yield. Agronomy Farm, Lincoln, Nebraska

DOE Office of Scientific and Technical Information (OSTI.GOV)

Power, J.F.

1981-01-01

Progress is reported in a study designed to evaluate the effects of quantity of crop residues left on soil surface on soil properties, plant growth, and crop yield and to determine the effects of quantity of surface residues upon soil, fertilizer, and residue N transformations, availability, and efficiency of use. In a dryland corn-sorghum-soybean rotation produced on a clay loam, residues remaining after harvest of the previous crop were removed and respread on plots at rates of 0, 0.5, 1.0, and 1.5 times the quantity of residues originally present. The above crops were planted in four replications the following springmore » without tillage, after broadcasting 50 kg N/ha as ammonium nitrate. In 1980, /sup 15/N-depleted NH/sub 4/NO/sub 3/ was applied to half of each plot. After harvest, crop residues produced on the half-plot receiving the N-isotope were transferred to the half-plot receiving regular fertilizer, and visa versa. In 1981, /sup 15/N-depleted NH/sub 4/NO/sub 3/ was applied to half of each plot again, except at right angles to the fertilizer applied in 1980. After planting each year, thermocouples were installed in each plot and soil temperatures were recorded. Also access tubes were installed in all plots and soil water content was measured to the 150 cm soil depth periodically during the growing season. Dry matter production and N uptake by the plant tissue was measured periodically during the growing season and at maturity. Additional measurements taken included leaf area index, xylem water potentials, and soil microbial populations. Data are presented on corn and soybean production characteristics as affected by rate of crop residue on soil surface. Results are also given on leaf area index (LAI) and dry matter production of corn and soybeans as affected by surface residue rate. Total N content of corn and soybean plant materials and surface residues, and total and inorganic soil N (1980) are reported.« less

Impact of accelerated plant growth on seed variety development

NASA Astrophysics Data System (ADS)

Christophersen, Eric

1998-01-01

The commercial lives of agricultural seed products have steadily declined in recent years. The introduction of genetically engineered crop seeds in 1966 has accentuated that trend. Widespread grower demand for genetically engineered seed requires competitive response by industry followers in order to avert market share losses to the industry leaders. Limitations on plant transformation technology, regulatory requirements and patent impediments require companies to rapidly convert transformed lines into elite commercial products. Massive multigenerational backcrossing efforts are required to distribute genetically engineered traits into a broad product mix. Significant incidents of expression failures, or ``gene silencing,'' have occurred unexpectedly, requiring product substitution strategies. First-to-market strategies, competitive response, broad germplasm conversion and rescue of product failures all share the element of urgency. Technologies which reliably accelerate product development rates can expect favorable reception by commercial seed developers. A growth chamber which dramatically accelerates the rate of plant growth is described.

Detailed monitoring of two biogas plants and mechanical solid-liquid separation of fermentation residues.

PubMed

Bauer, Alexander; Mayr, Herwig; Hopfner-Sixt, Katharina; Amon, Thomas

2009-06-01

The Austrian "green electricity act" (Okostromgesetz) has led to an increase in biogas power plant size and consequently to an increased use of biomass. A biogas power plant with a generating capacity of 500 kW(el) consumes up to 38,000 kg of biomass per day. 260 ha of cropland is required to produce this mass. The high water content of biomass necessitates a high transport volume for energy crops and fermentation residues. The transport and application of fermentation residues to farmland is the last step in this logistic chain. The use of fermentation residues as fertilizer closes the nutrient cycle and is a central element in the efficient use of biomass for power production. Treatment of fermentation residues by separation into liquid and solid phases may be a solution to the transport problem. This paper presents detailed results from the monitoring of two biogas plants and from the analysis of the separation of fermentation residues. Furthermore, two different separator technologies for the separation of fermentation residues of biogas plants were analyzed. The examined biogas plants correspond to the current technological state of the art and have designs developed specifically for the utilization of energy crops. The hydraulic retention time ranged between 45.0 and 83.7 days. The specific methane yields were 0.40-0.43 m(3)N CH(4) per kg VS. The volume loads ranged between 3.69 and 4.00 kg VS/m(3). The degree of degradation was between 77.3% and 82.14%. The screw extractor separator was better suited for biogas slurry separation than the rotary screen separator. The screw extractor separator exhibited a high throughput and good separation efficiency. The efficiency of slurry separation depended on the dry matter content of the fermentation residue. The higher the dry matter content, the higher the proportion of solid phase after separation. In this project, we found that the fermentation residues could be divided into 79.2% fluid phase with a dry matter

Effect of burn residue proximity on growth of 5 planted mixed-conifer species after 6 years

Treesearch

Robert A. York; Robert C. Heald

2007-01-01

Burned areas represent a significant amount of the forest landscape that can potentially be planted following site preparation techniques that use burning of woody residue. However, managers implementing post-harvest or post-wildfire regeneration efforts face uncertainty in expected performance of seedlings planted in or around post-burn residues (i.e. ash substrates...

The MARS15-based FermiCORD code system for calculation of the accelerator-induced residual dose

DOE Office of Scientific and Technical Information (OSTI.GOV)

Grebe, A.; Leveling, A.; Lu, T.

The FermiCORD code system, a set of codes based on MARS15 that calculates the accelerator-induced residual doses at experimental facilities of arbitrary configurations, has been developed. FermiCORD is written in C++ as an add-on to Fortran-based MARS15. The FermiCORD algorithm consists of two stages: 1) simulation of residual doses on contact with the surfaces surrounding the studied location and of radionuclide inventories in the structures surrounding those locations using MARS15, and 2) simulation of the emission of the nuclear decay gamma-quanta by the residuals in the activated structures and scoring the prompt doses of these gamma-quanta at arbitrary distances frommore » those structures. The FermiCORD code system has been benchmarked against similar algorithms based on other code systems and showed a good agreement. The code system has been applied for calculation of the residual dose of the target station for the Mu2e experiment and the results have been compared to approximate dosimetric approaches.« less

The MARS15-based FermiCORD code system for calculation of the accelerator-induced residual dose

NASA Astrophysics Data System (ADS)

Grebe, A.; Leveling, A.; Lu, T.; Mokhov, N.; Pronskikh, V.

2018-01-01

The FermiCORD code system, a set of codes based on MARS15 that calculates the accelerator-induced residual doses at experimental facilities of arbitrary configurations, has been developed. FermiCORD is written in C++ as an add-on to Fortran-based MARS15. The FermiCORD algorithm consists of two stages: 1) simulation of residual doses on contact with the surfaces surrounding the studied location and of radionuclide inventories in the structures surrounding those locations using MARS15, and 2) simulation of the emission of the nuclear decay γ-quanta by the residuals in the activated structures and scoring the prompt doses of these γ-quanta at arbitrary distances from those structures. The FermiCORD code system has been benchmarked against similar algorithms based on other code systems and against experimental data from the CERF facility at CERN, and FermiCORD showed reasonable agreement with these. The code system has been applied for calculation of the residual dose of the target station for the Mu2e experiment and the results have been compared to approximate dosimetric approaches.

Accelerated increase in plant species richness on mountain summits is linked to warming.

PubMed

Steinbauer, Manuel J; Grytnes, John-Arvid; Jurasinski, Gerald; Kulonen, Aino; Lenoir, Jonathan; Pauli, Harald; Rixen, Christian; Winkler, Manuela; Bardy-Durchhalter, Manfred; Barni, Elena; Bjorkman, Anne D; Breiner, Frank T; Burg, Sarah; Czortek, Patryk; Dawes, Melissa A; Delimat, Anna; Dullinger, Stefan; Erschbamer, Brigitta; Felde, Vivian A; Fernández-Arberas, Olatz; Fossheim, Kjetil F; Gómez-García, Daniel; Georges, Damien; Grindrud, Erlend T; Haider, Sylvia; Haugum, Siri V; Henriksen, Hanne; Herreros, María J; Jaroszewicz, Bogdan; Jaroszynska, Francesca; Kanka, Robert; Kapfer, Jutta; Klanderud, Kari; Kühn, Ingolf; Lamprecht, Andrea; Matteodo, Magali; di Cella, Umberto Morra; Normand, Signe; Odland, Arvid; Olsen, Siri L; Palacio, Sara; Petey, Martina; Piscová, Veronika; Sedlakova, Blazena; Steinbauer, Klaus; Stöckli, Veronika; Svenning, Jens-Christian; Teppa, Guido; Theurillat, Jean-Paul; Vittoz, Pascal; Woodin, Sarah J; Zimmermann, Niklaus E; Wipf, Sonja

2018-04-01

Globally accelerating trends in societal development and human environmental impacts since the mid-twentieth century 1-7 are known as the Great Acceleration and have been discussed as a key indicator of the onset of the Anthropocene epoch 6 . While reports on ecological responses (for example, changes in species range or local extinctions) to the Great Acceleration are multiplying 8, 9 , it is unknown whether such biotic responses are undergoing a similar acceleration over time. This knowledge gap stems from the limited availability of time series data on biodiversity changes across large temporal and geographical extents. Here we use a dataset of repeated plant surveys from 302 mountain summits across Europe, spanning 145 years of observation, to assess the temporal trajectory of mountain biodiversity changes as a globally coherent imprint of the Anthropocene. We find a continent-wide acceleration in the rate of increase in plant species richness, with five times as much species enrichment between 2007 and 2016 as fifty years ago, between 1957 and 1966. This acceleration is strikingly synchronized with accelerated global warming and is not linked to alternative global change drivers. The accelerating increases in species richness on mountain summits across this broad spatial extent demonstrate that acceleration in climate-induced biotic change is occurring even in remote places on Earth, with potentially far-ranging consequences not only for biodiversity, but also for ecosystem functioning and services.

Plant Food Residues as a Source of Nutraceuticals and Functional Foods.

PubMed

Varzakas, Theodoros; Zakynthinos, George; Verpoort, Francis

2016-12-10

This chapter describes the use of different plant and vegetable food residues as nutraceuticals and functional foods. Different nutraceuticals are mentioned and explained. Their uses are well addressed along with their disease management and their action as nutraceutical delivery vehicles.

The leaching kinetics of cadmium from hazardous Cu-Cd zinc plant residues.

PubMed

Li, Meng; Zheng, Shili; Liu, Biao; Du, Hao; Dreisinger, David Bruce; Tafaghodi, Leili; Zhang, Yi

2017-07-01

A large amount of Cu-Cd zinc plant residues (CZPR) are produced from the hydrometallurgical zinc plant operations. Since these residues contain substantial amount of heavy metals including Cd, Zn and Cu, therefore, they are considered as hazardous wastes. In order to realize decontamination treatment and efficient extraction of the valuable metals from the CZPR, a comprehensive recovery process using sulfuric acid as the leaching reagent and air as the oxidizing reagent has been proposed. The effect of temperature, sulfuric acid concentration, particle size, solid/liquid ratio and stirring speed on the cadmium extraction efficiency was investigated. The leaching kinetics of cadmium was also studied. It was concluded that the cadmium leaching process was controlled by the solid film diffusion process. Moreover, the order of the reaction rate constant versus H 2 SO 4 concentration, particle size, solid/liquid ratio and stirring speed was calculated. The XRD and SEM-EDS analysis results showed that the main phases of the secondary sulfuric acid leaching residues were lead sulfate and calcium sulfate. Copyright © 2017 Elsevier Ltd. All rights reserved.

Accelerating the degradation of green plant waste with chemical decomposition agents.

PubMed

Kejun, Sun; Juntao, Zhang; Ying, Chen; Zongwen, Liao; Lin, Ruan; Cong, Liu

2011-10-01

Degradation of green plant waste is often difficult, and excess maturity times are typically required. In this study, we used lignin, cellulose and hemicellulose assays; scanning electron microscopy; infrared spectrum analysis and X-ray diffraction analysis to investigate the effects of chemical decomposition agents on the lignocellulose content of green plant waste, its structure and major functional groups and the mechanism of accelerated degradation. Our results showed that adding chemical decomposition agents to Ficus microcarpa var. pusillifolia sawdust reduced the contents of lignin by 0.53%-11.48% and the contents of cellulose by 2.86%-7.71%, and increased the contents of hemicellulose by 2.92%-33.63% after 24 h. With increasing quantities of alkaline residue and sodium lignosulphonate, the lignin content decreased. Scanning electron microscopy showed that, after F. microcarpa var. pusillifolia sawdust was treated with chemical decomposition agents, lignocellulose tube wall thickness increased significantlyIncreases of 29.41%, 3.53% and 34.71% were observed after treatment with NaOH, alkaline residue and sodium lignosulphonate, respectively. Infrared spectroscopy showed that CO and aromatic skeleton stretching absorption peaks were weakened and the C-H vibrational absorption peak from out-of-plane in positions 2 and 6 (S units) (890-900 cm(-1)) was strengthened after F. microcarpa var. pusillifolia sawdust was treated with chemical decomposition agents, indicating a reduction in lignin content. Several absorption peaks [i.e., C-H deformations (asymmetry in methyl groups, -CH(3)- and -CH(2)-) (1450-1460 cm(-1)); Aliphatic C-H stretching in methyl and phenol OH (1370-1380 cm(-1)); CO stretching (cellulose and hemicellulose) (1040-1060 cm(-1))] that indicate the presence of a chemical bond between lignin and cellulose was reduced, indicating that the chemical bond between lignin and cellulose had been partially broken. X-ray diffraction analysis showed that Na

Plant Food Residues as a Source of Nutraceuticals and Functional Foods

PubMed Central

Varzakas, Theodoros; Zakynthinos, George; Verpoort, Francis

2016-01-01

This chapter describes the use of different plant and vegetable food residues as nutraceuticals and functional foods. Different nutraceuticals are mentioned and explained. Their uses are well addressed along with their disease management and their action as nutraceutical delivery vehicles. PMID:28231183

Physical and chemical characterization of residual oil-fired power plant emissions

EPA Science Inventory

Although the toxicity of oil combustion emissions is a significant public health concern, few studies characterize the emissions from plant-scale utility boilers firing residual oil. This study remedies that deficiency by sampling and monitoring stack emissions from a 432 Giga Jo...

Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils.

PubMed

Ma, Y; Prasad, M N V; Rajkumar, M; Freitas, H

2011-01-01

Technogenic activities (industrial-plastic, textiles, microelectronics, wood preservatives; mining-mine refuse, tailings, smelting; agrochemicals-chemical fertilizers, farm yard manure, pesticides; aerosols-pyrometallurgical and automobile exhausts; biosolids-sewage sludge, domestic waste; fly ash-coal combustion products) are the primary sources of heavy metal contamination and pollution in the environment in addition to geogenic sources. During the last two decades, bioremediation has emerged as a potential tool to clean up the metal-contaminated/polluted environment. Exclusively derived processes by plants alone (phytoremediation) are time-consuming. Further, high levels of pollutants pose toxicity to the remediating plants. This situation could be ameliorated and accelerated by exploring the partnership of plant-microbe, which would improve the plant growth by facilitating the sequestration of toxic heavy metals. Plants can bioconcentrate (phytoextraction) as well as bioimmobilize or inactivate (phytostabilization) toxic heavy metals through in situ rhizospheric processes. The mobility and bioavailability of heavy metal in the soil, particularly at the rhizosphere where root uptake or exclusion takes place, are critical factors that affect phytoextraction and phytostabilization. Developing new methods for either enhancing (phytoextraction) or reducing the bioavailability of metal contaminants in the rhizosphere (phytostabilization) as well as improving plant establishment, growth, and health could significantly speed up the process of bioremediation techniques. In this review, we have highlighted the role of plant growth promoting rhizo- and/or endophytic bacteria in accelerating phytoremediation derived benefits in extensive tables and elaborate schematic sketches. Copyright © 2010 Elsevier Inc. All rights reserved.

Effect of tillage and crop residue on soil temperature following planting for a Black soil in Northeast China.

PubMed

Shen, Yan; McLaughlin, Neil; Zhang, Xiaoping; Xu, Minggang; Liang, Aizhen

2018-03-14

Crop residue return is imperative to maintain soil health and productivity but some farmers resist adopting conservation tillage systems with residue return fearing reduced soil temperature following planting and crop yield. Soil temperatures were measured at 10 cm depth for one month following planting from 2004 to 2007 in a field experiment in Northeast China. Tillage treatments included mouldboard plough (MP), no till (NT), and ridge till (RT) with maize (Zea mays L.) and soybean (Glycine max Merr.) crops. Tillage had significant effects on soil temperature in 10 of 15 weekly periods. Weekly average NT soil temperature was 0-1.5 °C lower than MP, but the difference was significant (P < 0.05) only in 2007 when residue was not returned in MP the previous autumn. RT showed no clear advantage over NT in increasing soil temperature. Higher residue coverage caused lower soil temperature; the effect was greater for maize than soybean residue. Residue type had significant effect on soil temperature in 9 of 15 weekly periods with 0-1.9 °C lower soil temperature under maize than soybean residue. Both tillage and residue had small but inconsistent effect on soil temperature following planting in Northeast China representative of a cool to temperate zone.

Detailed analysis of Honeywell In-Space Accelerometer data - STS-32. [crystal microstructure response to different types of residual acceleration

NASA Technical Reports Server (NTRS)

Rogers, Melissa J. B.; Alexander, J. I. D.; Schoess, Jeff

1993-01-01

The Honeywell In-Space Accelerometer (HISA) system collected data in the mid-deck area of the Shuttle Columbia during the flight of STS-32, January 1990. The resulting data were to be used to investigate the response of crystal microstructure to different types of residual acceleration. The HISA is designed to detect and record transient and oscillatory accelerations. The sampling and electronics package stored averaged accelerations over two sampling periods; two sampling rates were available: 1 Hz and 50 Hz. Analysis of the HISA data followed the CMMR Acceleration Data Processing Guide, considering in-house computer modelling of a float-zone indium crystal growth experiment. Characteristic examples of HISA data showing the response to the primary reaction control system, Orbiter Maneuvering System operations, and crew treadmill activity are presented. Various orbiter structural modes are excited by these and other activities.

Accelerator-based conversion (ABC) of weapons plutonium: Plant layout study and related design issues

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cowell, B.S.; Fontana, M.H.; Krakowski, R.A.

1995-04-01

In preparation for and in support of a detailed R and D Plan for the Accelerator-Based Conversion (ABC) of weapons plutonium, an ABC Plant Layout Study was conducted at the level of a pre-conceptual engineering design. The plant layout is based on an adaptation of the Molten-Salt Breeder Reactor (MSBR) detailed conceptual design that was completed in the early 1070s. Although the ABC Plant Layout Study included the Accelerator Equipment as an essential element, the engineering assessment focused primarily on the Target; Primary System (blanket and all systems containing plutonium-bearing fuel salt); the Heat-Removal System (secondary-coolant-salt and supercritical-steam systems); Chemicalmore » Processing; Operation and Maintenance; Containment and Safety; and Instrumentation and Control systems. Although constrained primarily to a reflection of an accelerator-driven (subcritical) variant of MSBR system, unique features and added flexibilities of the ABC suggest improved or alternative approaches to each of the above-listed subsystems; these, along with the key technical issues in need of resolution through a detailed R&D plan for ABC are described on the bases of the ``strawman`` or ``point-of-departure`` plant layout that resulted from this study.« less

Identifying Plant Part Composition of Forest Logging Residue Using Infrared Spectral Data and Linear Discriminant Analysis

PubMed Central

Acquah, Gifty E.; Via, Brian K.; Billor, Nedret; Fasina, Oladiran O.; Eckhardt, Lori G.

2016-01-01

As new markets, technologies and economies evolve in the low carbon bioeconomy, forest logging residue, a largely untapped renewable resource will play a vital role. The feedstock can however be variable depending on plant species and plant part component. This heterogeneity can influence the physical, chemical and thermochemical properties of the material, and thus the final yield and quality of products. Although it is challenging to control compositional variability of a batch of feedstock, it is feasible to monitor this heterogeneity and make the necessary changes in process parameters. Such a system will be a first step towards optimization, quality assurance and cost-effectiveness of processes in the emerging biofuel/chemical industry. The objective of this study was therefore to qualitatively classify forest logging residue made up of different plant parts using both near infrared spectroscopy (NIRS) and Fourier transform infrared spectroscopy (FTIRS) together with linear discriminant analysis (LDA). Forest logging residue harvested from several Pinus taeda (loblolly pine) plantations in Alabama, USA, were classified into three plant part components: clean wood, wood and bark and slash (i.e., limbs and foliage). Five-fold cross-validated linear discriminant functions had classification accuracies of over 96% for both NIRS and FTIRS based models. An extra factor/principal component (PC) was however needed to achieve this in FTIRS modeling. Analysis of factor loadings of both NIR and FTIR spectra showed that, the statistically different amount of cellulose in the three plant part components of logging residue contributed to their initial separation. This study demonstrated that NIR or FTIR spectroscopy coupled with PCA and LDA has the potential to be used as a high throughput tool in classifying the plant part makeup of a batch of forest logging residue feedstock. Thus, NIR/FTIR could be employed as a tool to rapidly probe/monitor the variability of forest

Escherichia coli Common Pilus (ECP) Targets Arabinosyl Residues in Plant Cell Walls to Mediate Adhesion to Fresh Produce Plants*

PubMed Central

Rossez, Yannick; Holmes, Ashleigh; Lodberg-Pedersen, Henriette; Birse, Louise; Marshall, Jacqueline; Willats, William G. T.; Toth, Ian K.; Holden, Nicola J.

2014-01-01

Outbreaks of verotoxigenic Escherichia coli are often associated with fresh produce. However, the molecular basis to adherence is unknown beyond ionic lipid-flagellum interactions in plant cell membranes. We demonstrate that arabinans present in different constituents of plant cell walls are targeted for adherence by E. coli common pilus (ECP; or meningitis-associated and temperature-regulated (Mat) fimbriae) for E. coli serotypes O157:H7 and O18:K1:H7. l-Arabinose is a common constituent of plant cell wall that is rarely found in other organisms, whereas ECP is widespread in E. coli and other environmental enteric species. ECP bound to oligosaccharides of at least arabinotriose or longer in a glycan array, plant cell wall pectic polysaccharides, and plant glycoproteins. Recognition overlapped with the antibody LM13, which binds arabinanase-sensitive pectic epitopes, and showed a preferential affinity for (1→5)-α-linked l-arabinosyl residues and longer chains of arabinan as demonstrated with the use of arabinan-degrading enzymes. Functional adherence in planta was mediated by the adhesin EcpD in combination with the structural subunit, EcpA, and expression was demonstrated with an ecpR–GFP fusion and ECP antibodies. Spinach was found to be enriched for ECP/LM13 targets compared with lettuce. Specific recognition of arabinosyl residues may help explain the persistence of E. coli in the wider environment and association of verotoxigenic E. coli with some fresh produce plants by exploitation of a glycan found only in plant, not animal, cells. PMID:25320086

Escherichia coli common pilus (ECP) targets arabinosyl residues in plant cell walls to mediate adhesion to fresh produce plants.

PubMed

Rossez, Yannick; Holmes, Ashleigh; Lodberg-Pedersen, Henriette; Birse, Louise; Marshall, Jacqueline; Willats, William G T; Toth, Ian K; Holden, Nicola J

2014-12-05

Outbreaks of verotoxigenic Escherichia coli are often associated with fresh produce. However, the molecular basis to adherence is unknown beyond ionic lipid-flagellum interactions in plant cell membranes. We demonstrate that arabinans present in different constituents of plant cell walls are targeted for adherence by E. coli common pilus (ECP; or meningitis-associated and temperature-regulated (Mat) fimbriae) for E. coli serotypes O157:H7 and O18:K1:H7. l-Arabinose is a common constituent of plant cell wall that is rarely found in other organisms, whereas ECP is widespread in E. coli and other environmental enteric species. ECP bound to oligosaccharides of at least arabinotriose or longer in a glycan array, plant cell wall pectic polysaccharides, and plant glycoproteins. Recognition overlapped with the antibody LM13, which binds arabinanase-sensitive pectic epitopes, and showed a preferential affinity for (1→5)-α-linked l-arabinosyl residues and longer chains of arabinan as demonstrated with the use of arabinan-degrading enzymes. Functional adherence in planta was mediated by the adhesin EcpD in combination with the structural subunit, EcpA, and expression was demonstrated with an ecpR-GFP fusion and ECP antibodies. Spinach was found to be enriched for ECP/LM13 targets compared with lettuce. Specific recognition of arabinosyl residues may help explain the persistence of E. coli in the wider environment and association of verotoxigenic E. coli with some fresh produce plants by exploitation of a glycan found only in plant, not animal, cells. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

Plant protection product residues in white grapes and wines of "Malvasia Istriana" produced in Istria.

PubMed

Baša Česnik, Helena; Velikonja Bolta, Špela; Bavčar, Dejan; Radeka, Sanja; Lisjak, Klemen

2016-12-01

Monitorting of plant protection product residues was performed in 12 grape and 66 wine samples of "Malvasia Istriana" variety, produced in Istria winegrowing region of Croatia and Slovenia. The samples were analysed for the presence of 169 different active compounds using two multiresidual analytical methods: gas chromatography coupled with mass spectrometry and liquid chromatography coupled with tandem mass spectrometry. Residues were found in 58.3% of all the inspected grape samples and in 28.8% of all the inspected wine samples. Beside that contents of residues in grapes were below 10% of maximum residue level values and they should not represent any risk for "Malvasia Istriana" grape or wine consumers.

Effect of residue combinations on plant uptake of nutrients and potentially toxic elements.

PubMed

Brännvall, Evelina; Nilsson, Malin; Sjöblom, Rolf; Skoglund, Nils; Kumpiene, Jurate

2014-01-01

The aim of the plant pot experiment was to evaluate potential environmental impacts of combined industrial residues to be used as soil fertilisers by analysing i) element availability in fly ash and biosolids mixed with soil both individual and in combination, ii) changes in element phytoavailability in soil fertilised with these materials and iii) impact of the fertilisers on plant growth and element uptake. Plant pot experiments were carried out, using soil to which fresh residue mixtures had been added. The results showed that element availability did not correlate with plant growth in the fertilised soil with. The largest concentrations of K (3534 mg/l), Mg (184 mg/l), P (1.8 mg/l), S (760 mg/l), Cu (0.39 mg/l) and Zn (0.58 mg/l) in soil pore water were found in the soil mixture with biosolids and MSWI fly ashes; however plants did not grow at all in mixtures containing the latter, most likely due to the high concentration of chlorides (82 g/kg in the leachate) in this ash. It is known that high salinity of soil can reduce germination by e.g. limiting water absorption by the seeds. The concentrations of As, Cd and Pb in grown plants were negligible in most of the soils and were below the instrument detection limit values. The proportions of biofuel fly ash and biosolids can be adjusted in order to balance the amount and availability of macronutrients, while the possible increase of potentially toxic elements in biomass is negligible seeing as the plant uptake of such elements was low. Copyright © 2013 Elsevier Ltd. All rights reserved.

Use of plant residues on growth of mycorrhizal seedlings of neem (Azadirachta indica A. Juss.).

PubMed

Monte Júnior, Inácio P; Maia, Leonor C; Silva, Fábio S B; Cavalcante, Uided M T

2012-02-01

Owing to its multiple uses in veterinary medicine, biofertilizers, pest control, etc., the commercial cultivation of neem (Azadirachta indica) has been increasing in various countries. The use of arbuscular mycorrhizal fungi (AMF) and plant by-products (composted leaves and residues of neem and sugarcane) for the propagation of seedlings can be an efficient alternative to stimulate plant growth, reducing the propagation time and conferring increased tolerance of plants to biotic and abiotic stresses. Therefore this study aimed to evaluate the effect of plant substrates and inoculation with AMF on the production of neem seedlings. Beneficial effects of the application of neem by-products to neem seedlings were observed on most of the variables analysed. However, the treatment with sugarcane cake did not improve the growth of neem seedlings. In general, the inoculation treatments using Glomus etunicatum in the composted neem substrates improved seedling growth. Neem by-products benefit the growth of seedlings of this plant under greenhouse conditions. Inoculation with G. etunicatum enhances plants growth mainly in substrates with residues of neem leaves, providing an alternative for the production of seedlings of this crop under nursery conditions, which can reduce the need for chemical fertilizers that impact the environment. Copyright © 2011 Society of Chemical Industry.

Function of the evolutionarily conserved plant methionine-S-sulfoxide reductase without the catalytic residue.

PubMed

Le, Dung Tien; Nguyen, Kim-Lien; Chu, Ha Duc; Vu, Nam Tuan; Pham, Thu Thi Ly; Tran, Lam-Son Phan

2018-05-28

In plants, two types of methionine sulfoxide reductase (MSR) exist, namely methionine-S-sulfoxide reductase (MSRA) and methionine-R-sulfoxide reductase (MSRB). These enzymes catalyze the reduction of methionine sulfoxides (MetO) back to methionine (Met) by a catalytic cysteine (Cys) and one or two resolving Cys residues. Interestingly, a group of MSRA encoded by plant genomes does not have a catalytic residue. We asked that if this group of MSRA did not have any function (as fitness), why it was not lost during the evolutionary process. To challenge this question, we analyzed the gene family encoding MSRA in soybean (GmMSRAs). We found seven genes encoding GmMSRAs, which included three segmental duplicated pairs. Among them, a pair of duplicated genes, namely GmMSRA1 and GmMSRA6, was without a catalytic Cys residue. Pseudogenes were ruled out as their transcripts were detected in various tissues and their Ka/Ks ratio indicated a negative selection pressure. In vivo analysis in Δ3MSR yeast strain indicated that the GmMSRA6 did not have activity toward MetO, contrasting to GmMSRA3 which had catalytic Cys and had activity. When exposed to H 2 O 2 -induced oxidative stress, GmMSRA6 did not confer any protection to the Δ3MSR yeast strain. Overexpression of GmMSRA6 in Arabidopsis thaliana did not alter the plant's phenotype under physiological conditions. However, the transgenic plants exhibited slightly higher sensitivity toward salinity-induced stress. Taken together, this data suggested that the plant MSRAs without the catalytic Cys are not enzymatically active and their existence may be explained by a role in regulating plant MSR activity via dominant-negative substrate competition mechanism.

A Comparison of Corn (Zea mays L.) Residue and Its Biochar on Soil C and Plant Growth

PubMed Central

Calderón, Francisco J.; Benjamin, Joseph; Vigil, Merle F.

2015-01-01

In order to properly determine the value of charring crop residues, the C use efficiency and effects on crop performance of biochar needs to be compared to the un-charred crop residues. In this study we compared the addition of corn stalks to soil, with equivalent additions of charred (300 °C and 500 °C) corn residues. Two experiments were conducted: a long term laboratory mineralization, and a growth chamber trial with proso millet plants. In the laboratory, we measured soil mineral N dynamics, C use efficiency, and soil organic matter (SOM) chemical changes via infrared spectroscopy. The 300 °C biochar decreased plant biomass relative to a nothing added control. The 500°C biochar had little to no effect on plant biomass. With incubation we measured lower soil NO3 content in the corn stalk treatment than in the biochar-amended soils, suggesting that the millet growth reduction in the stalk treatment was mainly driven by N limitation, whereas other factors contributed to the biomass yield reductions in the biochar treatments. Corn stalks had a C sequestration use efficiency of up to 0.26, but charring enhanced C sequestration to values that ranged from 0.64 to 1.0. Infrared spectroscopy of the soils as they mineralized showed that absorbance at 3400, 2925-2850, 1737 cm-1, and 1656 cm-1 decreased during the incubation and can be regarded as labile SOM, corn residue, or biochar bands. Absorbances near 1600, 1500-1420, and 1345 cm-1 represented the more refractory SOM moieties. Our results show that adding crop residue biochar to soil is a sound C sequestration technology compared to letting the crop residues decompose in the field. This is because the resistance to decomposition of the chars after soil amendment offsets any C losses during charring of the crop residues. PMID:25836653

A comparison of corn (Zea mays L.) residue and its biochar on soil C and plant growth.

PubMed

Calderón, Francisco J; Benjamin, Joseph; Vigil, Merle F

2015-01-01

In order to properly determine the value of charring crop residues, the C use efficiency and effects on crop performance of biochar needs to be compared to the un-charred crop residues. In this study we compared the addition of corn stalks to soil, with equivalent additions of charred (300 °C and 500 °C) corn residues. Two experiments were conducted: a long term laboratory mineralization, and a growth chamber trial with proso millet plants. In the laboratory, we measured soil mineral N dynamics, C use efficiency, and soil organic matter (SOM) chemical changes via infrared spectroscopy. The 300 °C biochar decreased plant biomass relative to a nothing added control. The 500°C biochar had little to no effect on plant biomass. With incubation we measured lower soil NO3 content in the corn stalk treatment than in the biochar-amended soils, suggesting that the millet growth reduction in the stalk treatment was mainly driven by N limitation, whereas other factors contributed to the biomass yield reductions in the biochar treatments. Corn stalks had a C sequestration use efficiency of up to 0.26, but charring enhanced C sequestration to values that ranged from 0.64 to 1.0. Infrared spectroscopy of the soils as they mineralized showed that absorbance at 3400, 2925-2850, 1737 cm-1, and 1656 cm-1 decreased during the incubation and can be regarded as labile SOM, corn residue, or biochar bands. Absorbances near 1600, 1500-1420, and 1345 cm-1 represented the more refractory SOM moieties. Our results show that adding crop residue biochar to soil is a sound C sequestration technology compared to letting the crop residues decompose in the field. This is because the resistance to decomposition of the chars after soil amendment offsets any C losses during charring of the crop residues.

Flow-accelerated corrosion in power plants. Revision 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chexal, B.; Horowitz, J.; Dooley, B.

1998-07-01

Flow-Accelerated Corrosion (FAC) is a phenomenon that results in metal loss from piping, vessels, and equipment made of carbon steel. FAC occurs only under certain conditions of flow, chemistry, geometry, and material. Unfortunately, those conditions are common in much of the high-energy piping in nuclear and fossil-fueled power plants. Undetected, FAC will cause leaks and ruptures. Consequently, FAC has become a major issue, particularly for nuclear plants. Although major failures are rare, the consequences can be severe. In 1986, four men in the area of an FAC-induced pipe rupture were killed. Fossil plants too, are subject to FAC. In 1995,more » a failure at a fossil-fired plant caused two fatalities. In addition to concerns about personnel safety, FAC failures can pose challenges to plant safety. Regulatory agencies have therefore required nuclear utilities to institute formal programs to address FAC. Finally, a major FAC failure (like the one that happened in 1997 at a US nuclear power plant) can force a plant to shutdown and purchase replacement power at a price approaching a million dollars per day depending upon the MWe rating of the plant. A great deal of time and money has been spent to develop the technology to predict, detect, and mitigate FAC in order to prevent catastrophic failures. Over time, substantial progress has been made towards understanding and preventing FAC. The results of these efforts include dozens of papers, reports, calculations, and manuals, as well as computer programs and other tools. This book is written to provide a detailed treatment of the entire subject in a single document. Any complex issue requires balancing know-how, the risk of decision making, and a pragmatic engineering solution. This book addresses these by carrying out the necessary R and D and engineering along with plant knowledge to cover all quadrants of Chexal`s four quadrant known-unknown diagram, as seen in Figure i.« less

Pyrolysis and reutilization of plant residues after phytoremediation of heavy metals contaminated sediments: For heavy metals stabilization and dye adsorption.

PubMed

Gong, Xiaomin; Huang, Danlian; Liu, Yunguo; Zeng, Guangming; Wang, Rongzhong; Wei, Jingjing; Huang, Chao; Xu, Piao; Wan, Jia; Zhang, Chen

2018-04-01

This study aimed to investigate the effect of pyrolysis on the stabilization of heavy metals in plant residues obtained after phytoremediation. Ramie residues, being collected after phytoremediation of metal contaminated sediments, were pyrolyzed at different temperatures (300-700 °C). Results indicated that pyrolysis was effective in the stabilization of Cd, Cr, Zn, Cu, and Pb in ramie residues by converting the acid-soluble fraction of metals into residual form and decreasing the TCLP-leachable metal contents. Meanwhile, the reutilization potential of using the pyrolysis products generated from ramie residues obtained after phytoremediation as sorbents was investigated. Adsorption experiments results revealed that the pyrolysis products presented excellent ability to adsorb methylene blue (MB) with a maximum adsorption capacity of 259.27 mg/g. This study demonstrated that pyrolysis could be used as an efficient alternative method for stabilizing heavy metals in plant residues obtained after phytoremediation, and their pyrolysis products could be reutilized for dye adsorption. Copyright © 2018 Elsevier Ltd. All rights reserved.

Dynamics of the Methanogenic Archaeal Community during Plant Residue Decomposition in an Anoxic Rice Field Soil ▿

PubMed Central

Peng, Jingjing; Lü, Zhe; Rui, Junpeng; Lu, Yahai

2008-01-01

Incorporation of plant residues strongly enhances the methane production and emission from flooded rice fields. Temperature and residue type are important factors that regulate residue decomposition and CH4 production. However, the response of the methanogenic archaeal community to these factors in rice field soil is not well understood. In the present experiment, the structure of the archaeal community was determined during the decomposition of rice root and straw residues in anoxic rice field soil incubated at three temperatures (15°C, 30°C, and 45°C). More CH4 was produced in the straw treatment than root treatment. Increasing the temperature from 15°C to 45°C enhanced CH4 production. Terminal restriction fragment length polymorphism analyses in combination with cloning and sequencing of 16S rRNA genes showed that Methanosarcinaceae developed early in the incubations, whereas Methanosaetaceae became more abundant in the later stages. Methanosarcinaceae and Methanosaetaceae seemed to be better adapted at 15°C and 30°C, respectively, while the thermophilic Methanobacteriales and rice cluster I methanogens were significantly enhanced at 45°C. Straw residues promoted the growth of Methanosarcinaceae, whereas the root residues favored Methanosaetaceae. In conclusion, our study revealed a highly dynamic structure of the methanogenic archaeal community during plant residue decomposition. The in situ concentration of acetate (and possibly of H2) seems to be the key factor that regulates the shift of methanogenic community. PMID:18344350

A conserved amino acid residue critical for product and substrate specificity in plant triterpene synthases

PubMed Central

Salmon, Melissa; Thimmappa, Ramesha B.; Minto, Robert E.; Melton, Rachel E.; O’Maille, Paul E.; Hemmings, Andrew M.; Osbourn, Anne

2016-01-01

Triterpenes are structurally complex plant natural products with numerous medicinal applications. They are synthesized through an origami-like process that involves cyclization of the linear 30 carbon precursor 2,3-oxidosqualene into different triterpene scaffolds. Here, through a forward genetic screen in planta, we identify a conserved amino acid residue that determines product specificity in triterpene synthases from diverse plant species. Mutation of this residue results in a major change in triterpene cyclization, with production of tetracyclic rather than pentacyclic products. The mutated enzymes also use the more highly oxygenated substrate dioxidosqualene in preference to 2,3-oxidosqualene when expressed in yeast. Our discoveries provide new insights into triterpene cyclization, revealing hidden functional diversity within triterpene synthases. They further open up opportunities to engineer novel oxygenated triterpene scaffolds by manipulating the precursor supply. PMID:27412861

Accelerator-based analytical technique in the study of some anti-diabetic medicinal plants of Nigeria

NASA Astrophysics Data System (ADS)

Olabanji, S. O.; Omobuwajo, O. R.; Ceccato, D.; Adebajo, A. C.; Buoso, M. C.; Moschini, G.

2008-05-01

Diabetes mellitus, a clinical syndrome characterized by hyperglycemia due to deficiency of insulin, is a disease involving the endocrine pancreas and causes considerable morbidity and mortality in the world. In Nigeria, many plants, especially those implicated in herbal recipes for the treatment of diabetes, have not been screened for their elemental constituents while information on phytochemistry of some of them is not available. There is therefore the need to document these constituents as some of these plants are becoming increasingly important as herbal drugs or food additives. The accelerator-based technique PIXE, using the 1.8 MeV collimated proton beam from the 2.5 MV AN 2000 Van de Graaff accelerator at INFN, LNL, Legnaro (Padova) Italy, was employed in the determination of the elemental constituents of these anti-diabetic medicinal plants. Leaves of Gardenia ternifolia, Caesalpina pulcherrima, Solemostenon monostachys, whole plant of Momordica charantia and leaf and stem bark of Hunteria umbellata could be taken as vegetables, neutraceuticals, food additives and supplements in the management of diabetes. However, Hexabolus monopetalus root should be used under prescription.

Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion.

PubMed

Zhang, Jian; Wang, Peng Cheng; Fang, Ling; Zhang, Qi-An; Yan, Cong Sheng; Chen, Jing Yi

2017-03-30

Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.

Microwave heating of tea residue yields polysaccharides, polyphenols, and plant biopolyester.

PubMed

Tsubaki, Shuntaro; Iida, Hiroyuki; Sakamoto, Masahiro; Azuma, Jun-ichi

2008-12-10

Microwave heating was used to produce aqueous-soluble components from green, oolong, and black tea residues. Heating at 200-230 degrees C for 2 min extracted 40-50% of polysaccharides and 60-70% of the polyphenols. Solubilization of arabinose and galactose by autohydrolysis occurred with heating above 170 degrees C, whereas heating above 200 degrees C was necessary to solubilize xylose. Catechins were soluble in water by heating at low temperature (110 degrees C); however, new polyphenols having strong antioxidant activity were produced above 200 degrees C. The amount of solubilized materials and antioxidant activity increased with increased fermentation of harvested tea leaves (green tea < oolong tea < black tea). Cutin, a plant biopolyester, remained in the residue after heating as did cellulose and lignin/tannin. The predominant cutin monomer that was recovered was 9,10-epoxy-18-hydroxyoctadecanoic acid, followed by dihydroxyhexadecanoic acid and 9,10,18-trihydroxyoctadecanoic acid.

Impact of imidacloprid residues on the development of Eisenia fetida during vermicomposting of greenhouse plant waste.

PubMed

Fernández-Gómez, Manuel J; Romero, Esperanza; Nogales, Rogelio

2011-09-15

Pesticide application in agriculture causes residues in post-harvest plant waste at different concentrations. Knowledge concerning how pesticide concentrations in such waste affect earthworms is essential for recycling greenhouse plant debris through vermicomposting. Here, we have evaluated the effects of imidacloprid (IMD) residues on earthworms (Eisenia fetida) during the vermicomposting of plant waste from greenhouse crops in Spain. Before, the effect of different IMD concentrations on earthworms was tested using cattle manure as an optimum waste for worm development. The results after using cattle manure indicate that IMD dose ≥ 5 mg kg(-1) hinders worm growth and even causes death, whereas IMD dose ≤ 2 mg IMD kg(-1) allows worm growth similar to control but impedes reproduction. The results from the vermicomposting of plant waste reveal that IMD inhibits adequate worm growth and increases mortality. Although 89% worms became sexually mature in substrate containing 2 mg IMD kg(-1), they did not produce cocoons. IMD also affected microorganisms harboured in the substrates for vermicomposting, as indicated by the reduction in their dehydrogenase activity. This enzyme activity was restored after vermicomposting. This study provides a sound basis for the vermicomposting of pesticide-contaminated plant waste. Copyright © 2011 Elsevier B.V. All rights reserved.

Analysis of Residual Acceleration Effects on Transport and Segregation During Directional Solidification of Tin-Bismuth in the MEPHISTO Furnace Facility

NASA Technical Reports Server (NTRS)

Alexander, J. Iwan D.

1998-01-01

The research accomplishments summarized in this Final Report during the period from 3/95 to 3/98, which included a 12 months no-cost extension granted at the end of the nominal 2 year period of performance. The report has 5 sections, in section 1 the objectives are presented, a task description is given and the background and significance of the work is outlined. In section 2 the research accomplishments are summarized. In section 3 publications and presentations are listed. Student participation is listed in 4. The work is summarized in section 5. and references for sections 1 and 2 are supplied in section 6. The object of this work, is to approach the problem of determining the transport conditions (and effects of residual acceleration) during the plane-front directional solidification of a tin-bismuth alloy under low gravity conditions. The work involved using a combination of 2- and 3-D numerical models, scaling analyses, ID models and the results of ground-based and low-gravity experiments. The experiments conducted in the MEPHISTO furnace facility during the USW-3 space flight which took place between February 22 through March 6, 199). This experiment represents an unprecedented opportunity to make a quantitative correlation between residual accelerations and the response of an actual experimental solidification

Short-term acclimation to warmer temperatures accelerates leaf carbon exchange processes across plant types.

PubMed

Smith, Nicholas G; Dukes, Jeffrey S

2017-11-01

While temperature responses of photosynthesis and plant respiration are known to acclimate over time in many species, few studies have been designed to directly compare process-level differences in acclimation capacity among plant types. We assessed short-term (7 day) temperature acclimation of the maximum rate of Rubisco carboxylation (V cmax ), the maximum rate of electron transport (J max ), the maximum rate of phosphoenolpyruvate carboxylase carboxylation (V pmax ), and foliar dark respiration (R d ) in 22 plant species that varied in lifespan (annual and perennial), photosynthetic pathway (C 3 and C 4 ), and climate of origin (tropical and nontropical) grown under fertilized, well-watered conditions. In general, acclimation to warmer temperatures increased the rate of each process. The relative increase in different photosynthetic processes varied by plant type, with C 3 species tending to preferentially accelerate CO 2 -limited photosynthetic processes and respiration and C 4 species tending to preferentially accelerate light-limited photosynthetic processes under warmer conditions. R d acclimation to warmer temperatures caused a reduction in temperature sensitivity that resulted in slower rates at high leaf temperatures. R d acclimation was similar across plant types. These results suggest that temperature acclimation of the biochemical processes that underlie plant carbon exchange is common across different plant types, but that acclimation to warmer temperatures tends to have a relatively greater positive effect on the processes most limiting to carbon assimilation, which differ by plant type. The acclimation responses observed here suggest that warmer conditions should lead to increased rates of carbon assimilation when water and nutrients are not limiting. © 2017 John Wiley & Sons Ltd.

The History And Future Direction Of Wastewater Treatment Plant Residuals Disinfection And Energy Recovery Considerations

EPA Science Inventory

Presentation will review the history of disinfection practices for wastewater treatment plant residuals and discuss both the development and limitations of the U.S. regulations. Present U.S. regulations protect human health by minimizing the contact of humans with infectious mic...

Removal of polycyclic aromatic hydrocarbons from aqueous solution by raw and modified plant residue materials as biosorbents.

PubMed

Xi, Zemin; Chen, Baoliang

2014-04-01

Removal of polycyclic aromatic hydrocarbons (PAHs), e.g., naphthalene, acenaphthene, phenanthrene and pyrene, from aqueous solution by raw and modified plant residues was investigated to develop low cost biosorbents for organic pollutant abatement. Bamboo wood, pine wood, pine needles and pine bark were selected as plant residues, and acid hydrolysis was used as an easily modification method. The raw and modified biosorbents were characterized by elemental analysis, Fourier transform infrared spectroscopy and scanning electron microscopy. The sorption isotherms of PAHs to raw biosorbents were apparently linear, and were dominated by a partitioning process. In comparison, the isotherms of the hydrolyzed biosorbents displayed nonlinearity, which was controlled by partitioning and the specific interaction mechanism. The sorption kinetic curves of PAHs to the raw and modified plant residues fit well with the pseudo second-order kinetics model. The sorption rates were faster for the raw biosorbents than the corresponding hydrolyzed biosorbents, which was attributed to the latter having more condensed domains (i.e., exposed aromatic core). By the consumption of the amorphous cellulose component under acid hydrolysis, the sorption capability of the hydrolyzed biosorbents was notably enhanced, i.e., 6-18 fold for phenanthrene, 6-8 fold for naphthalene and pyrene and 5-8 fold for acenaphthene. The sorption coefficients (Kd) were negatively correlated with the polarity index [(O+N)/C], and positively correlated with the aromaticity of the biosorbents. For a given biosorbent, a positive linear correlation between logKoc and logKow for different PAHs was observed. Interestingly, the linear plots of logKoc-logKow were parallel for different biosorbents. These observations suggest that the raw and modified plant residues have great potential as biosorbents to remove PAHs from wastewater. Copyright © 2014 The Research Centre for Eco-Environmental Sciences, Chinese Academy of

Study of Methanogenesis while Bioutilisation of Plant Residuals

NASA Astrophysics Data System (ADS)

Ilyin, V. K.; Korniushenkova, I. N.; Starkova, L. V.; Lauriniavichius, K. S.

respect principals of planet ecology, and compatibility with other habitability systems. For these purpose the waste management technologies, relevant to application of the biodegradation properties of bacteria are of great value. Biological treatment method is based upon the biodegradation of organic substances by various microorganisms. vegetable non-edible residual, using artificial inoculum; to study peculiarities of biogas, possibilities to optimize or to reduce the share of methane. fermentation. The biogas production achieved 46 l per 1 kg of substrate. The microbial studies of biodegradation process revealed following peculiarities: (i)gradual quantitative increasing of Lactobacillus sp. (from 103 to 105 colony forming units (CFU) per ml); (ii)activation of Clostridia sp. (from 102 to 104 CFU/ml); (iii) elimination of aerobic conventional pathogens (Enterobacteriaceae sp., Protea sp., staphylococci). methane content measures revealed traces 0.1-0.4%. granules, the amount of methane in biogas reached 80-90%. biodegradation of vegetable wastes. This inoculum consists of active sludge adapted to wastes mixed with excretes of insects which consume plant wastes. Using this inoculum the biodegradation process takes less time, then that using active sludge. Regulation of methane concentration from traces to 90% may be achieved by adding of methane reactor to the plant digester.

Investigation of the possibility of using residual heat reactor energy

NASA Astrophysics Data System (ADS)

Aminov, R. Z.; Yurin, V. E.; Bessonov, V. N.

2017-11-01

The largest contribution to the probable frequency of core damage is blackout events. The main component of the heat capacity at each reactor within a few minutes following a blackout is the heat resulting from the braking of beta-particles and the transfer of gamma-ray energy by the fission fragments and their decay products, which is known as the residual heat. The power of the residual heat changes gradually over a long period of time and for a VVER-1000 reactor is about 15-20 MW of thermal power over 72 hours. Current cooldown systems increase the cost of the basic nuclear power plants (NPP) funds without changing the amount of electricity generated. Such systems remain on standby, accelerating the aging of the equipment and accordingly reducing its reliability. The probability of system failure increases with the duration of idle time. Furthermore, the reactor residual heat energy is not used. A proposed system for cooling nuclear power plants involves the use of residual thermal power to supply the station’s own needs in emergency situations accompanied by a complete blackout. The thermal power of residual heat can be converted to electrical energy through an additional low power steam turbine. In normal mode, the additional steam turbine generates electricity, which makes it possible to ensure spare NPP and a return on the investment in the reservation system. In this work, experimental data obtained from a Balakovo NPP was analyzed to determine the admissibility of cooldown of the reactors through the 2nd circuit over a long time period, while maintaining high-level parameters for the steam generated by the steam generators.

Dietary exposure to pesticide residues from foods of plant origin and drinks in Lebanon.

PubMed

Nasreddine, Lara; Rehaime, Maria; Kassaify, Zeina; Rechmany, Roula; Jaber, Farouk

2016-08-01

This study assesses the dietary exposure of Lebanese adults to 47 pesticide residues from both foods of plant origin and drinks. The study was conducted using the Total Diet Study protocol in two different areas of Lebanon: Greater Beirut (urban) and Keserwan (semi-rural). A total of 1860 individual foods were collected, prepared, and cooked prior to analysis. Composite samples of similar foods were analyzed, following the QuEChERS Multiresidue method. Eighteen residues were detected/quantified on at least one composite sample, with 66.7 % of the results being quantifiable and 33.3 % detectable. Quantifiable levels ranged between 10.3 and 208 μg/kg. For the composite samples where residues were detected, 55 % had one residue, while 45 % had 2-4 residues. The most frequently detected/quantified pesticide residues included Chlorpyrifos, Procymidone, Primiphos methyl, Dimethoate, and Dieldrin. The dietary exposure assessment was conducted using the deterministic approach with two scenarios: (1) the lower bound (LB) approach and (2) the upper bound (UB) approach. Using the LB approach, mean estimated daily exposures were far below the acceptable daily intakes (ADIs) for all investigated residues. Using the UB approach, which tends to overestimate exposure, mean estimated daily exposures were below the ADIs for all residues except for Dieldrin (semi-rural: 128.7 % ADI; urban: 100.7 % ADI). Estimates of mean exposure to Diazinon reached 50.3 % of ADI in the urban diet and 61.9 % in the semi-rural diet. Findings of this study identify specific pesticide residues as monitoring priorities for which more comprehensive and sensitive analyses are needed in order to refine exposure assessment.

Residual effects of EDDS leachates on plants during EDDS-assisted phytoremediation of copper contaminated soil.

PubMed

Yang, Li; Luo, Chunling; Liu, Yue; Quan, Lingtong; Chen, Yahua; Shen, Zhenguo

2013-02-01

In this study, a novel experimental setup (one pot placed above another) was used to investigate the residual effects of EDDS application on plant growth and metal uptake. Two plant species, garland chrysanthemum and ryegrass, were grown in the upper pots (mimicking the upper soil layers) and were harvested 7 days after EDDS application. During this period the upper pots were watered twice. The lower pots (mimicking the subsoil under the upper soil layers) served as leachate collectors. Thereafter, the two pots were separated, and the same plants were grown in the upper and lower pots in two continuous croppings. Results showed that EDDS application restrained the growth of the first crop and resulted in a dramatic enhancement of Cu accumulation in plants grown in the upper pots. However, no negative growth effects were identified for the second and third crops, which were harvested 81 and 204 days after the EDDS application, respectively. In the lower pots, the leachate from the upper pots after EDDS application exhibited the increased total and CaCl(2)-extractable Cu concentrations in the soil. However, the growth of garland chrysanthemum and ryegrass, and their shoot Cu concentrations were unaffected. These data suggest that the residual risk associated with EDDS application was limited, and that subsoil to which EDDS leachate was applied may exhibit reduced Cu bioavailability for plants due to the biodegradation of EDDS. Copyright © 2012 Elsevier B.V. All rights reserved.

Evaluating the fate of metals in air pollution control residues from coal-fired power plants

EPA Science Inventory

Changes in air pollution control at coal-fired power plants are shifting mercury (Hg) and other metals from the flue gas at electric utilities to the coal ash. This paper presents data from the characterization of73 coal combustion residues (CCRs) evaluating the composition and c...

Multiple climate drivers accelerate Arctic plant community senescence

NASA Astrophysics Data System (ADS)

Livensperger, C.; Steltzer, H.; Wallenstein, M. D.; Weintraub, M. N.

2015-12-01

Alteration of seasonal phenology cues due to climate change has led to changes in the onset and duration of the growing season. While photoperiod often acts as an ultimate control on phenological events, recent studies have shown that environmental cues such as temperature and soil water content can modify the direction and rate of senescence processes. Warmer temperatures have resulted in an observed trend towards delayed senescence across temperate latitudes. However, Arctic regions are characterized by extreme seasonality and rapidly decreasing photoperiod, and consequently senescence may not shift as climate warms. We monitored the timing of Arctic plant community senescence for three years under the framework of an experimental manipulation that altered seasonal phenological cues through warming and earlier snowmelt. Alternative models of senescence were tested to determine if microclimate (air temperature, soil temperature, and soil moisture) or start of season phenology affect the timing and rate of community senescence. We found that all three microclimate predictors contributed to explaining variation in timing of senescence, suggesting that photoperiod is not the sole control on timing of senescence in Arctic plant communities. Rather, increased air and soil temperatures along with drier soil conditions, led to acceleration in the onset of senescence at a community level. Our data suggest that (1) multiple climate drivers predict timing of plant community senescence, and (2) climate change could result in a shorter peak season due to earlier onset of senescence, which would decrease the potential carbon uptake in moist acidic tundra.

Soil Organic Matter Quality of an Oxisol Affected by Plant Residues and Crop Sequence under No-Tillage

NASA Astrophysics Data System (ADS)

Cora, Jose; Marcelo, Adolfo

2013-04-01

Plant residues are considered the primarily resource for soil organic matter (SOM) formation and the amounts and properties of plant litter are important controlling factors for the SOM quality. We determined the amounts, quality and decomposition rate of plant residues and the effects of summer and winter crop sequences on soil organic C (TOC) content, both particulate organic C (POC) and mineral-associated organic C (MOC) pools and humic substances in a Brazilian Rhodic Eutrudox soil under a no-tillage system. The organic C analysis in specifics pools used in this study was effective and should be adopted in tropical climates to evaluate the soil quality and the sustainability of various cropping systems. Continuous growth of soybean (Glycine max L. Merrill) on summer provided higher contents of soil POC and continuous growth of maize (Zea mays L.) provided higher soil humic acid and MOC contents. Summer soybean-maize rotation provided the higher plant diversity, which likely improved the soil microbial activity and the soil organic C consumption. The winter sunn hemp (Crotalaria juncea L.), pigeon pea (Cajanus cajan (L.) Millsp), oilseed radish (Raphanus sativus L.) and pearl millet (Pennisetum americanum (L.) Leeke) enhanced the soil MOC, a finding that is attributable to the higher N content of the crop residue. Sunn hemp and pigeon pea provided the higher soil POC content. Sunn hemp showed better performance and positive effects on the SOM quality, making it a suitable winter crop choice for tropical conditions with a warm and dry winter.

Carbon sequestration in dryland soils and plant residue as influenced by tillage and crop rotation.

PubMed

Sainju, Upendra M; Lenssen, Andrew; Caesar-Thonthat, Thecan; Waddell, Jed

2006-01-01

Long-term use of conventional tillage and wheat (Triticum aestivum L.)-fallow systems in the northern Great Plains have resulted in low soil organic carbon (SOC) levels. We examined the effects of two tillage practices [conventional till (CT) and no-till (NT)], five crop rotations [continuous spring wheat (CW), spring wheat-fallow (W-F), spring wheat-lentil (Lens culinaris Medic.) (W-L), spring wheat-spring wheat-fallow (W-W-F), and spring wheat-pea (Pisum sativum L.)-fallow (W-P-F)], and Conservation Reserve Program (CRP) planting on plant C input, SOC, and particulate organic carbon (POC). A field experiment was conducted in a mixture of Scobey clay loam (fine-loamy, mixed, Aridic Argiborolls) and Kevin clay loam (fine, montmorillonitic, Aridic Argiborolls) from 1998 to 2003 in Havre, MT. Total plant biomass returned to the soil from 1998 to 2003 was greater in CW (15.5 Mg ha(-1)) than in other rotations. Residue cover, amount, and C content in 2004 were 33 to 86% greater in NT than in CT and greater in CRP than in crop rotations. Residue amount (2.47 Mg ha(-1)) and C content (0.96 Mg ha(-1)) were greater in NT with CW than in other treatments, except in CT with CRP and W-F and in NT with CRP and W-W-F. The SOC at the 0- to 5-cm depth was 23% greater in NT (6.4 Mg ha(-1)) than in CT. The POC was not influenced by tillage and crop rotation, but POC to SOC ratio at the 0- to 20-cm depth was greater in NT with W-L (369 g kg(-1) SOC) than in CT with CW, W-F, and W-L. From 1998 to 2003, SOC at the 0- to 20-cm depth decreased by 4% in CT but increased by 3% in NT. Carbon can be sequestered in dryland soils and plant residue in areas previously under CRP using reduced tillage and increased cropping intensity, such as NT with CW, compared with traditional practice, such as CT with W-F system, and the content can be similar to that in CRP planting.

Metal-accelerated oxidation in plant cell death

DOE Office of Scientific and Technical Information (OSTI.GOV)

Czuba, M.

1993-05-01

Cadmium and mercury toxicity is further enhanced by external oxidizing conditions O[sub 3] or inherent plant processes. Lepidium sativum L, Lycopersicon esculentum Mill., or Phaseolus vulgaris L, were grown inpeat-lite to maturity under continuous cadmium exposure followed by one oxidant (O[sub 3]-6 hr. 30 pphm) exposure, with or without foliar calcium pretreatments. In comparison, Daucus carota, L and other species grown in a 71-V suspension, with or without 2,4-D were exposed continuously to low levels of methylmercury during exponential growth and analyzed in aggregates of distinct populations. Proteins were extracted and analyzed. Mechanisms of toxicity and eventual cell death aremore » Ca-mediated and involve chloroplast, stomatal-water relations and changes in oxidant-anti-oxidant components in cells. Whether the metal-accelerated oxidative damage proceeds to cell death, depends on the species and its differential biotransformation system and cell association component.« less

Pesticide residues in nut-planted soils of China and their relationship between nut/soil.

PubMed

Han, Yongxiang; Mo, Runhong; Yuan, Xinyue; Zhong, Donglian; Tang, Fubin; Ye, Caifen; Liu, Yihua

2017-08-01

Twenty-nine pesticide residues in nut-planted soils from China were investigated. One organophosphate (chlorpyrifos) was detected in 5.3% soils, and the residue levels of 7.2 μg/kg to 77.2 μg/kg. The concentrations of six organochlorines (DDT, HCH, endosulfan, quintozene, aldrin and dieldrin) detected in 78.9% soils were 0.6 μg/kg to 90.1 μg/kg. The residue levels of six pyrethroids (bifenthrin, fenpropathrin, cyhalothrin, cypermethrin, fenvalerate and deltamethrin) detected in 65.8% soils were 1.5 μg/kg to 884.3 μg/kg. Triadimefon and buprofezin were found in 71.1% and 52.6% samples, respectively, with the corresponding concentrations of 9.8 μg/kg to 193.7 μg/kg and 87.9 μg/kg to 807.4 μg/kg. The multiple residues were found in 76.3% soils. A significant correlation between pesticide residues in nuts and soils was observed, with the correlation coefficient (r) 0.83 (P < 0.001). In addition, the bioconcentration factor (BCF) values for the explanation of pesticides from soils into nuts were ranged from 0.8 to 16.5. The results showed that some pesticides could accumulate in nut by the uptake effect from soil. Copyright © 2017 Elsevier Ltd. All rights reserved.

40 CFR 180.181 - Chlorpropham; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

... residues of the plant regulator and herbicide chlorpropham (isopropyl m-chlorocarbanilate (CIPC) in or on...) Tolerances are established for the combined residues of the plant regulator and herbicide chlorpropham...

40 CFR 180.181 - Chlorpropham; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

... residues of the plant regulator and herbicide chlorpropham (isopropyl m-chlorocarbanilate (CIPC) in or on...) Tolerances are established for the combined residues of the plant regulator and herbicide chlorpropham...

40 CFR 180.181 - Chlorpropham; tolerances for residues.

Code of Federal Regulations, 2011 CFR

2011-07-01

... residues of the plant regulator and herbicide chlorpropham (isopropyl m-chlorocarbanilate (CIPC) in or on...) Tolerances are established for the combined residues of the plant regulator and herbicide chlorpropham...

40 CFR 180.181 - Chlorpropham; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

... residues of the plant regulator and herbicide chlorpropham (isopropyl m-chlorocarbanilate (CIPC) in or on...) Tolerances are established for the combined residues of the plant regulator and herbicide chlorpropham...

40 CFR 180.181 - Chlorpropham; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

... residues of the plant regulator and herbicide chlorpropham (isopropyl m-chlorocarbanilate (CIPC) in or on...) Tolerances are established for the combined residues of the plant regulator and herbicide chlorpropham...

Saprobe fungi decreased the sensitivity to the toxic effect of dry olive mill residue on arbuscular mycorrhizal plants.

PubMed

Sampedro, I; Aranda, E; Díaz, R; García-Sanchez, M; Ocampo, J A; García-Romera, I

2008-02-01

We studied the influence of olive mill dry residue (DOR) treated with saprobe fungi on growth of tomato and alfalfa colonized by Glomus deserticola. The application of 25g kg(-1) of dry DOR to soil decreased the shoot and root dry weight of tomato and alfalfa. Plants were more sensitive to the toxicity of DOR when colonized with the arbuscular mycorrhizal (AM) fungi. The sensitivity of both plants to the toxicity of DOR differed according to whether they were colonized by G. deserticola or by indigenous AM fungi. The phytotoxicity of DOR towards tomato and alfalfa was decreased by incubation the residue before planting with saprobe fungi for 20wk. The beneficial effects of AM fungi on plant growth added with DOR incubated with saprobe fungi depend of the type of the plant and AM fungi. The contribution of AM fungi to the beneficial effect of DOR incubated with saprobe fungi varied according to the type of the plant and AM fungi. G. deserticola increased the shoot and root dry weight of plants when they were grown in the presence of DOR incubated with saprobe fungi for 20wk. The beneficial effect of saprobe fungi on the dry weight and the level of AM mycorrhization of plants seem to be related to the decrease caused by these fungi in the phenol concentration in DOR. However, the toxicity of DOR due to substances other than phenols can not be ignored. The use of certain saprobe and AM fungi allows the possibility of using DOR as an organic fertilizer.

RAMI modeling of selected balance of plant systems for the proposed Accelerator Production of Tritium (APT) project

DOE Office of Scientific and Technical Information (OSTI.GOV)

Radder, J.A.; Cramer, D.S.

1997-06-01

In order to meet Department of Energy (DOE) Defense Program requirements for tritium in the 2005-2007 time frame, new production capability must be made available. The Accelerator Production of Tritium (APT) Plant is being considered as an alternative to nuclear reactor production of tritium, which has been the preferred method in the past. The proposed APT plant will use a high-power proton accelerator to generate thermal neutrons that will be captured in {sup 3}He to produce tritium (3H). It is expected that the APT Plant will be built and operated at the DOE`s Savannah River Site (SRS) in Aiken, Southmore » Carolina. Discussion is focused on Reliability, Availability, Maintainability, and Inspectability (RAMI) modeling of recent conceptual designs for balance of plant (BOP) systems in the proposed APT Plant. In the conceptual designs for balance of plant (BOP) systems in the proposed APT Plant. In the conceptual design phase, system RAMI estimates are necessary to identify the best possible system alternative and to provide a valid picture of the cost effectiveness of the proposed system for comparison with other system alternatives. RAMI estimates in the phase must necessarily be based on generic data. The objective of the RAMI analyses at the conceptual design stage is to assist the designers in achieving an optimum design which balances the reliability and maintainability requirements among the subsystems and components.« less

Fractionation and leachability of heavy metals from aged and recent Zn metallurgical leach residues from the Três Marias zinc plant (Minas Gerais, Brazil).

PubMed

Sethurajan, Manivannan; Huguenot, David; Lens, Piet N L; Horn, Heinrich A; Figueiredo, Luiz H A; van Hullebusch, Eric D

2016-04-01

Various mineral processing operations to produce pure metals from mineral ores generate sludges, residues, and other unwanted by-products/wastes. As a general practice, these wastes are either stored in a reservoir or disposed in the surrounding of mining/smelting areas, which might cause adverse environmental impacts. Therefore, it is important to understand the various characteristics like heavy metal leaching features and potential toxicity of these metallurgical wastes. In this study, zinc plant leach residues (ZLRs) were collected from a currently operating Zn metallurgical industry located in Minas Gerais (Brazil) and investigated for their potential toxicity, fractionation, and leachability. Three different ZLR samples (ZLR1, ZLR2, and ZLR3) were collected, based on their age of production and deposition. They mainly consisted of Fe (6-11.5 %), Zn (2.5 to 5.0 %), and Pb (1.5 to 2.5 %) and minor concentrations of Al, Cd, Cu, and Mn, depending on the sample age. Toxicity Characteristic Leaching Procedure (TCLP) results revealed that these wastes are hazardous for the environment. Accelerated Community Bureau of Reference (BCR) sequential extraction clearly showed that potentially toxic heavy metals such as Cd, Cu, Pb, and Zn can be released into the environment in high quantities under mild acidic conditions. The results of the liquid-solid partitioning as a function of pH showed that pH plays an important role in the leachability of metals from these residues. At low pH (pH 2.5), high concentrations of metals can be leached: 67, 25, and 7 % of Zn can be leached from leach residues ZLR1, ZLR2, and ZLR3, respectively. The release of metals decreased with increasing pH. Geochemical modeling of the pH-dependent leaching was also performed to determine which geochemical process controls the leachability/solubility of the heavy metals. This study showed that the studied ZLRs contain significant concentrations of non-residual extractable fractions of Zn and can

40 CFR 180.569 - Forchlorfenuron; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

... residues of the plant growth regulator forchlorfenuron; N-(2-chloro-4-pyridinyl)-N′phenyl urea in or on the....06 Kiwifruit 0.04 (2) Time-limited tolerances are established for residues of the plant growth...

40 CFR 180.569 - Forchlorfenuron; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

... residues of the plant growth regulator forchlorfenuron; N-(2-chloro-4-pyridinyl)-N′phenyl urea in or on the....06 Kiwifruit 0.04 (2) Time-limited tolerances are established for residues of the plant growth...

40 CFR 180.569 - Forchlorfenuron; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

... residues of the plant growth regulator forchlorfenuron; N-(2-chloro-4-pyridinyl)-N′phenyl urea in or on the....06 Kiwifruit 0.04 (2) Time-limited tolerances are established for residues of the plant growth...

40 CFR 180.569 - Forchlorfenuron; tolerances for residues.

Code of Federal Regulations, 2011 CFR

2011-07-01

... residues of the plant growth regulator forchlorfenuron; N-(2-chloro-4-pyridinyl)-N′phenyl urea in or on the....06 Kiwifruit 0.04 (2) Time-limited tolerances are established for residues of the plant growth...

Biogas production from vietnamese animal manure, plant residues and organic waste: influence of biomass composition on methane yield.

PubMed

Cu, T T T; Nguyen, T X; Triolo, J M; Pedersen, L; Le, V D; Le, P D; Sommer, S G

2015-02-01

Anaerobic digestion is an efficient and renewable energy technology that can produce biogas from a variety of biomasses such as animal manure, food waste and plant residues. In developing countries this technology is widely used for the production of biogas using local biomasses, but there is little information about the value of these biomasses for energy production. This study was therefore carried out with the objective of estimating the biogas production potential of typical Vietnamese biomasses such as animal manure, slaughterhouse waste and plant residues, and developing a model that relates methane (CH4) production to the chemical characteristics of the biomass. The biochemical methane potential (BMP) and biomass characteristics were measured. Results showed that piglet manure produced the highest CH4 yield of 443 normal litter (NL) CH4 kg(-1) volatile solids (VS) compared to 222 from cows, 177 from sows, 172 from rabbits, 169 from goats and 153 from buffaloes. Methane production from duckweed (Spirodela polyrrhiza) was higher than from lawn grass and water spinach at 340, 220, and 110.6 NL CH4 kg(-1) VS, respectively. The BMP experiment also demonstrated that the CH4 production was inhibited with chicken manure, slaughterhouse waste, cassava residue and shoe-making waste. Statistical analysis showed that lipid and lignin are the most significant predictors of BMP. The model was developed from knowledge that the BMP was related to biomass content of lipid, lignin and protein from manure and plant residues as a percentage of VS with coefficient of determination (R-square) at 0.95. This model was applied to calculate the CH4 yield for a household with 17 fattening pigs in the highlands and lowlands of northern Vietnam.

Biogas Production from Vietnamese Animal Manure, Plant Residues and Organic Waste: Influence of Biomass Composition on Methane Yield

PubMed Central

Cu, T. T. T.; Nguyen, T. X.; Triolo, J. M.; Pedersen, L.; Le, V. D.; Le, P. D.; Sommer, S. G.

2015-01-01

Anaerobic digestion is an efficient and renewable energy technology that can produce biogas from a variety of biomasses such as animal manure, food waste and plant residues. In developing countries this technology is widely used for the production of biogas using local biomasses, but there is little information about the value of these biomasses for energy production. This study was therefore carried out with the objective of estimating the biogas production potential of typical Vietnamese biomasses such as animal manure, slaughterhouse waste and plant residues, and developing a model that relates methane (CH4) production to the chemical characteristics of the biomass. The biochemical methane potential (BMP) and biomass characteristics were measured. Results showed that piglet manure produced the highest CH4 yield of 443 normal litter (NL) CH4 kg−1 volatile solids (VS) compared to 222 from cows, 177 from sows, 172 from rabbits, 169 from goats and 153 from buffaloes. Methane production from duckweed (Spirodela polyrrhiza) was higher than from lawn grass and water spinach at 340, 220, and 110.6 NL CH4 kg−1 VS, respectively. The BMP experiment also demonstrated that the CH4 production was inhibited with chicken manure, slaughterhouse waste, cassava residue and shoe-making waste. Statistical analysis showed that lipid and lignin are the most significant predictors of BMP. The model was developed from knowledge that the BMP was related to biomass content of lipid, lignin and protein from manure and plant residues as a percentage of VS with coefficient of determination (R-square) at 0.95. This model was applied to calculate the CH4 yield for a household with 17 fattening pigs in the highlands and lowlands of northern Vietnam. PMID:25557826

Effects of rapeseed residue on lead and cadmium availability and uptake by rice plants in heavy metal contaminated paddy soil.

PubMed

Ok, Yong Sik; Usman, Adel R A; Lee, Sang Soo; Abd El-Azeem, Samy A M; Choi, Bongsu; Hashimoto, Yohey; Yang, Jae E

2011-10-01

Rapeseed (Brassica napus L.) has been cultivated for biodiesel production worldwide. Winter rapeseed is commonly grown in the southern part of Korea under a rice-rapeseed double cropping system. In this study, a greenhouse pot experiment was conducted to assess the effects of rapeseed residue applied as a green manure alone or in combinations with mineral N fertilizer on Cd and Pb speciation in the contaminated paddy soil and their availability to rice plant (Oryza sativa L.). The changes in soil chemical and biological properties in response to the addition of rapeseed residue were also evaluated. Specifically, the following four treatments were evaluated: 100% mineral N fertilizer (N100) as a control, 70% mineral N fertilizer+rapeseed residue (N70+R), 30% mineral N fertilizer+rapeseed residue (N30+R) and rapeseed residue alone (R). The electrical conductivity and exchangeable cations of the rice paddy soil subjected to the R treatment or in combinations with mineral N fertilizer treatment, N70+R and N30+R, were higher than those in soils subjected to the N100 treatment. However, the soil pH value with the R treatment (pH 6.3) was lower than that with N100 treatment (pH 6.9). Use of rapeseed residue as a green manure led to an increase in soil organic matter (SOM) and enhanced the microbial populations in the soil. Sequential extraction also revealed that the addition of rapeseed residue decreased the easily accessible fraction of Cd by 5-14% and Pb by 30-39% through the transformation into less accessible fractions, thereby reducing metal availability to the rice plant. Overall, the incorporation of rapeseed residue into the metal contaminated rice paddy soils may sustain SOM, improve the soil chemical and biological properties, and decrease the heavy metal phytoavailability. Copyright © 2011 Elsevier Ltd. All rights reserved.

TENORM: Wastewater Treatment Residuals

EPA Pesticide Factsheets

Water and wastes which have been discharged into municipal sewers are treated at wastewater treatment plants. These may contain trace amounts of both man-made and naturally occurring radionuclides which can accumulate in the treatment plant and residuals.

Preparation of Grinding Aid Using Waste Acid Residue from Plasticizer Plant

NASA Astrophysics Data System (ADS)

Li, Lingxiao; Feng, Yanchao; Liu, Manchao; Zhao, Fengqing

2017-09-01

The grinding aid for granulated blast-furnace slag were prepared from waste acid residue from plasticizer plant through neutralization, de-methanol and granulation process. In this process, sulfuric acid was transformed into gypsum which has much contribution for grinding effect by combined use with the glycerol and poly glycerin in the waste. Fly ash was used for granulation for the composite grinding aid. Methanol can be recycled in the process. The result showed that the suitable addition of grinding aid is 0.03 % of granulated blast-furnace slag (mass). In this case, the specific surface area is 14% higher than that of the blank. Compared with the common grinding aids, it has excellent performance and low cost.

The effect of motorcycle helmet fit on estimating head impact kinematics from residual liner crush.

PubMed

Bonin, Stephanie J; Gardiner, John C; Onar-Thomas, Arzu; Asfour, Shihab S; Siegmund, Gunter P

2017-09-01

Proper helmet fit is important for optimizing head protection during an impact, yet many motorcyclists wear helmets that do not properly fit their heads. The goals of this study are i) to quantify how a mismatch in headform size and motorcycle helmet size affects headform peak acceleration and head injury criteria (HIC), and ii) to determine if peak acceleration, HIC, and impact speed can be estimated from the foam liner's maximum residual crush depth or residual crush volume. Shorty-style helmets (4 sizes of a single model) were tested on instrumented headforms (4 sizes) during linear impacts between 2.0 and 10.5m/s to the forehead region. Helmets were CT scanned to quantify residual crush depth and volume. Separate linear regression models were used to quantify how the response variables (peak acceleration (g), HIC, and impact speed (m/s)) were related to the predictor variables (maximum crush depth (mm), crush volume (cm 3 ), and the difference in circumference between the helmet and headform (cm)). Overall, we found that increasingly oversized helmets reduced peak headform acceleration and HIC for a given impact speed for maximum residual crush depths less than 7.9mm and residual crush volume less than 40cm 3 . Below these levels of residual crush, we found that peak headform acceleration, HIC, and impact speed can be estimated from a helmet's residual crush. Above these crush thresholds, large variations in headform kinematics are present, possibly related to densification of the foam liner during the impact. Copyright © 2017 Elsevier Ltd. All rights reserved.

Membrane topology and identification of key residues of EaDAcT, a plant MBOAT with unusual substrate specificity.

PubMed

Tran, Tam N T; Shelton, Jennifer; Brown, Susan; Durrett, Timothy P

2017-10-01

Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) catalyzes the transfer of an acetyl group from acetyl-CoA to the sn-3 position of diacylglycerol to form 3-acetyl-1,2-diacyl-sn-glycerol (acetyl-TAG). EaDAcT belongs to a small, plant-specific subfamily of the membrane bound O-acyltransferases (MBOAT) that acylate different lipid substrates. Sucrose gradient density centrifugation revealed that EaDAcT colocalizes to the same fractions as an endoplasmic reticulum (ER)-specific marker. By mapping the membrane topology of EaDAcT, we obtained an experimentally determined topology model for a plant MBOAT. The EaDAcT model contains four transmembrane domains (TMDs), with both the N- and C-termini orientated toward the lumen of the ER. In addition, there is a large cytoplasmic loop between the first and second TMDs, with the MBOAT signature region of the protein embedded in the third TMD close to the interface between the membrane and the cytoplasm. During topology mapping, we discovered two cysteine residues (C187 and C293) located on opposite sides of the membrane that are important for enzyme activity. In order to identify additional amino acid residues important for acetyltransferase activity, we isolated and characterized acetyltransferases from other acetyl-TAG-producing plants. Among them, the acetyltransferase from Euonymus fortunei possessed the highest activity in vivo and in vitro. Mutagenesis of conserved amino acids revealed that S253, H257, D258 and V263 are essential for EaDAcT activity. Alteration of residues unique to the acetyltransferases did not alter the unique acyl donor specificity of EaDAcT, suggesting that multiple amino acids are important for substrate recognition. © 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd.

Residual Field Correction of Pulsed Bending Magnet

NASA Astrophysics Data System (ADS)

Takano, Junpei; Igarashi, Susumu; Kamikubota, Norihiko; Meigo, Shin-ichiro; Sato, Kenichi; Shirakata, Masashi; Yamada, Shuei

The Japan Proton Accelerator Research Complex (J-PARC) has an accelerator chain, Linac, Rapid Cycling Synchrotron (RCS), and Main Ring (MR). The RCS accelerates the proton beam up to 3 GeV every 40 msec. After the beam is extracted from the RCS, it is delivered to a beam transport line, which is 3NBT for the Material and Life Science Experimental Facility (MLF). Some bunches of the proton beam are bended from the 3NBT to another beam transport line, which is 3-50BT for the MR, by using a pulsed bending magnet (PB) [1]. However, the beam orbit in the 3NBT is kicked by the residual magnetic field of the PB. In order to correct the residual magnetic field, additional coils had been wound on the PB poles. As a result of scanning the current pattern of the correction coils, the orbit distortion in the 3NBT has been reduced.

A method for accelerated trait conversion in plant breeding.

PubMed

Lewis, Ramsey S; Kernodle, S P

2009-05-01

Backcrossing is often used in cultivar development to transfer one or a few genes to desired genetic backgrounds. The duration necessary to complete such 'trait conversions' is largely dependent upon generation times. Constitutive overexpression of the Arabidopsis thaliana gene FT (FLOWERING LOCUS T) induces early-flowering in many plants. Here, we used tobacco (Nicotiana tabacum L.) as a model system to propose and examine aspects of a modified backcross procedure where transgenic FT overexpression is used to reduce generation time and accelerate gene transfer. In this method, the breeder would select for an FT transgene insertion and the trait(s) of interest at each backcross generation except the last. In the final generation, selection would be conducted for the trait(s) of interest, but against FT, to generate the backcross-derived trait conversion. We demonstrate here that constitutive FT overexpression functions to dramatically reduce days-to-flower similarly in diverse tobacco genetic backgrounds. FT-containing plants flowered in an average of 39 days, in comparison with 87-138 days for non-FT plants. Two FT transgene insertions were found to segregate independently of several disease resistance genes often the focus of backcrossing in tobacco. In addition, no undesirable epigenetic effects on flowering time were observed once FT was segregated away. The proposed system would reduce the time required to complete a trait conversion in tobacco by nearly one-half. These features suggest the possible value of this modified backcrossing system for tobacco or other crop species where long generation times or photoperiod sensitivity may impede timely trait conversion.

Residual Stress Analysis Based on Acoustic and Optical Methods.

PubMed

Yoshida, Sanichiro; Sasaki, Tomohiro; Usui, Masaru; Sakamoto, Shuichi; Gurney, David; Park, Ik-Keun

2016-02-16

Co-application of acoustoelasticity and optical interferometry to residual stress analysis is discussed. The underlying idea is to combine the advantages of both methods. Acoustoelasticity is capable of evaluating a residual stress absolutely but it is a single point measurement. Optical interferometry is able to measure deformation yielding two-dimensional, full-field data, but it is not suitable for absolute evaluation of residual stresses. By theoretically relating the deformation data to residual stresses, and calibrating it with absolute residual stress evaluated at a reference point, it is possible to measure residual stresses quantitatively, nondestructively and two-dimensionally. The feasibility of the idea has been tested with a butt-jointed dissimilar plate specimen. A steel plate 18.5 mm wide, 50 mm long and 3.37 mm thick is braze-jointed to a cemented carbide plate of the same dimension along the 18.5 mm-side. Acoustoelasticity evaluates the elastic modulus at reference points via acoustic velocity measurement. A tensile load is applied to the specimen at a constant pulling rate in a stress range substantially lower than the yield stress. Optical interferometry measures the resulting acceleration field. Based on the theory of harmonic oscillation, the acceleration field is correlated to compressive and tensile residual stresses qualitatively. The acoustic and optical results show reasonable agreement in the compressive and tensile residual stresses, indicating the feasibility of the idea.

Roles of histidine residues in plant vacuolar H(+)-pyrophosphatase.

PubMed

Hsiao, Yi Y; Van, Ru C; Hung, Shu H; Lin, Hsin H; Pan, Rong L

2004-02-15

Vacuolar proton pumping pyrophosphatase (H(+)-PPase; EC 3.6.1.1) plays a pivotal role in electrogenic translocation of protons from cytosol to the vacuolar lumen at the expense of PP(i) hydrolysis. Alignment analysis on amino acid sequence demonstrates that vacuolar H(+)-PPase of mung bean contains six highly conserved histidine residues. Previous evidence indicated possible involvement of histidine residue(s) in enzymatic activity and H(+)-translocation of vacuolar H(+)-PPase as determined by using histidine specific modifier, diethylpyrocarbonate [J. Protein Chem. 21 (2002) 51]. In this study, we further attempted to identify the roles of histidine residues in mung bean vacuolar H(+)-PPase by site-directed mutagenesis. A line of mutants with histidine residues singly replaced by alanine was constructed, over-expressed in Saccharomyces cerevisiae, and then used to determine their enzymatic activities and proton translocations. Among the mutants scrutinized, only the mutation of H716 significantly decreased the enzymatic activity, the proton transport, and the coupling ratio of vacuolar H(+)-PPase. The enzymatic activity of H716A is relatively resistant to inhibition by diethylpyrocarbonate as compared to wild-type and other mutants, indicating that H716 is probably the target residue for the attack by this modifier. The mutation at H716 of V-PPase shifted the optimum pH value but not the T(1/2) (pretreatment temperature at which half enzymatic activity is observed) for PP(i) hydrolytic activity. Mutation of histidine residues obviously induced conformational changes of vacuolar H(+)-PPase as determined by immunoblotting analysis after limited trypsin digestion. Furthermore, mutation of these histidine residues modified the inhibitory effects of F(-) and Na(+), but not that of Ca(2+). Single substitution of H704, H716 and H758 by alanine partially released the effect of K(+) stimulation, indicating possible location of K(+) binding in the vicinity of domains

Multiresidue method for pesticide residue analysis in food of animal and plant origin based on GC or LC and MS or MS/MS.

PubMed

Muñoz, Eva; Muñoz, Gloria; Pineda, Laura; Serrahima, Eulalia; Centrich, Francesc

2012-01-01

A multiresidue method based on GC or LC and MS or MS/MS for the determination of 204 pesticides in diverse food matrixes of animal and plant origin is described. The method can include different stages of cleanup according to the chemical characteristics of each sample. Samples were extracted using accelerated solvent extraction. Those with a high fat content or that contained chlorophyll required further purification by gel permeation chromatography and/or SPE (ENVI-Carb). The methodology developed here was fully validated; the LOQs for the 204 pesticides are presented. The LOQ values lie between 0.01 to 0.02 mg/kg. However, in some cases, mainly in baby food, they were as low as 0.003 mg/kg, thereby meeting European Union requirements on maximum residue levels for pesticides, as outlined in European regulation 396/2005 and the Commission Directive 2003/13/EC. The procedure has been accredited for a wide scope of pesticides and matrixes by the Spanish Accreditation Body (ENAC) following ISO/IEC 17025:2005, as outlined in ENAC technical note NT-19.

Accurate Measurement of Velocity and Acceleration of Seismic Vibrations near Nuclear Power Plants

NASA Astrophysics Data System (ADS)

Arif, Syed Javed; Imdadullah; Asghar, Mohammad Syed Jamil

In spite of all prerequisite geological study based precautions, the sites of nuclear power plants are also susceptible to seismic vibrations and their consequent effects. The effect of the ongoing nuclear tragedy in Japan caused by an earthquake and its consequent tsunami on March 11, 2011 is currently beyond contemplations. It has led to a rethinking on nuclear power stations by various governments around the world. Therefore, the prediction of location and time of large earthquakes has regained a great importance. The earth crust is made up of several wide, thin and rigid plates like blocks which are in constant motion with respect to each other. A series of vibrations on the earth surface are produced by the generation of elastic seismic waves due to sudden rupture within the plates during the release of accumulated strain energy. The range of frequency of seismic vibrations is from 0 to 10 Hz. However, there appears a large variation in magnitude, velocity and acceleration of these vibrations. The response of existing or conventional methods of measurement of seismic vibrations is very slow, which is of the order of tens of seconds. A systematic and high resolution measurement of velocity and acceleration of these vibrations are useful to interpret the pattern of waves and their anomalies more accurately, which are useful for the prediction of an earthquake. In the proposed work, a fast rotating magnetic field (RMF) is used to measure the velocity and acceleration of seismic vibrations in the millisecond range. The broad spectrum of pulses within one second range, measured by proposed method, gives all possible values of instantaneous velocity and instantaneous acceleration of the seismic vibrations. The spectrum of pulses in millisecond range becomes available which is useful to measure the pattern of fore shocks to predict the time and location of large earthquakes more accurately. Moreover, instead of average, the peak values of these quantities are helpful

Characterization and elimination of undesirable protein residues in plant cell walls for enhancing lignin analysis by solution-state 2D gel-NMR methods

USDA-ARS?s Scientific Manuscript database

Proteins exist in every plant cell wall. Certain protein residues interfere with lignin characterization and quantification. The current solution-state 2D-NMR technique (gel-NMR) for whole plant cell wall structural profiling provides detailed information regarding cell walls and proteins. However, ...

On the ASR and ASR thermal residues characterization of full scale treatment plant.

PubMed

Mancini, G; Viotti, P; Luciano, A; Fino, D

2014-02-01

In order to obtain 85% recycling, several procedures on Automotive Shredder Residue (ASR) could be implemented, such as advanced metal and polymer recovery, mechanical recycling, pyrolysis, the direct use of ASR in the cement industry, and/or the direct use of ASR as a secondary raw material. However, many of these recovery options appear to be limited, due to the possible low acceptability of ASR based products on the market. The recovery of bottom ash and slag after an ASR thermal treatment is an option that is not usually considered in most countries (e.g. Italy) due to the excessive amount of contaminants, especially metals. The purpose of this paper is to provide information on the characteristics of ASR and its full-scale incineration residues. Experiments have been carried out, in two different experimental campaigns, in a full-scale tyre incineration plant specifically modified to treat ASR waste. Detailed analysis of ASR samples and combustion residues were carried out and compared with literature data. On the basis of the analytical results, the slag and bottom ash from the combustion process have been classified as non-hazardous wastes, according to the EU waste acceptance criteria (WAC), and therefore after further tests could be used in future in the construction industry. It has also been concluded that ASR bottom ash (EWC - European Waste Catalogue - code 19 01 12) could be landfilled in SNRHW (stabilized non-reactive hazardous waste) cells or used as raw material for road construction, with or without further treatment for the removal of heavy metals. In the case of fly ash from boiler or Air Pollution Control (APC) residues, it has been found that the Cd, Pb and Zn concentrations exceeded regulatory leaching test limits therefore their removal, or a stabilization process, would be essential prior to landfilling the use of these residues as construction material. Copyright © 2013 Elsevier Ltd. All rights reserved.

Study of methanogenesis during bioutilization of plant residuals

NASA Astrophysics Data System (ADS)

Ilyin, V. K.; Korniushenkova, I. N.; Starkova, L. V.; Lauriniavichius, K. S.

2005-02-01

The waste management strategy for the future should meet the benefits of human safety, respect principles of planet ecology, and compatibility with other habitability systems. For these purposes waste management technologies relevant to application of the biodegradation properties of bacteria are of great value. Biological treatment method is based on the biodegradation of organic substances by various microorganisms. The objectives of our study were: to evaluate the effectiveness of microbial biodegradation of vegetable non-edible residual, using artificial inoculum, and to study the peculiarities of biogas, and possibilities of optimizing or reducing the share of methane. The diminution rate of organic gained 76% from initial mass within 9 days of fermentation. The biogas production achieved 46 l/kg of substrate. The microbial studies of biodegradation process revealed the following peculiarities: (i) gradual quantitative increase of Lactobacillus sp. (from 103 to 105 colony-forming units (CFU) per ml); (ii) activation of Clostridia sp. (from 102 to 10 4 CFU/ml); and (iii) elimination of aerobic conventional pathogens (Enterobacteriaceae, Protea sp., Staphylococci). Chromatography analysis revealed the constant presence of carbon dioxide (up to 90.9%). The methane content measures revealed traces 0.1-0.4%. However, when we optimized the methane production in "boiling layer" using methanogenic granules, the amount of methane in biogas reached 80-90%. Based on the results obtained the artificial inoculum was created which was capable of initiating biodegradation of vegetable wastes. This inoculum consisted of active sludge adapted to wastes mixed with excretea of insects which consume plant wastes. Using this inoculum the biodegradation process takes less time than that using active sludge. Regulation of methane concentration from traces to 90% may be achieved by adding methane reactor to the plant digester.

40 CFR 180.226 - Diquat; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

.... (3) Tolerances are established for the plant growth regulator diquat (6,7 dihydrodipyrido(1,2-a:2'1... Diquat; tolerances for residues. (a) General. (1) Tolerances are established for residues of the plant growth regulator and herbicide diquat, (6,7-dihydrodipyrido (1,2-a:2′1′-c)pyrazinediium) derived from...

40 CFR 180.226 - Diquat; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

.... (3) Tolerances are established for the plant growth regulator diquat (6,7 dihydrodipyrido(1,2-a:2'1... Diquat; tolerances for residues. (a) General. (1) Tolerances are established for residues of the plant growth regulator and herbicide diquat, (6,7-dihydrodipyrido (1,2-a:2′1′-c)pyrazinediium) derived from...

40 CFR 180.226 - Diquat; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

.... (3) Tolerances are established for the plant growth regulator diquat (6,7 dihydrodipyrido(1,2-a:2'1... Diquat; tolerances for residues. (a) General. (1) Tolerances are established for residues of the plant growth regulator and herbicide diquat, (6,7-dihydrodipyrido (1,2-a:2′1′-c)pyrazinediium) derived from...

Pesticides residues and metals in plant products from agricultural area of Belgrade, Serbia.

PubMed

Ethorđević, Tijana; Ethurović, Rada

2012-03-01

The objective of study was to assess the levels of selected metals and pesticides in plant products from agricultural area of Belgrade, Serbia in order to indicate their possible sources and risks of contamination and to evaluate their sanitary probity and safety. The concentrations of cadmium, copper, iron, manganese, nickel, lead and zinc were below limits established by national and international regulations (maximum found concentrations were 0.028, 1.91, 11.16, 1.77, 0.605, 0.073 and 1.76 mg kg(-1) respectively). Only residue of one of examined pesticides was found in amount below MRL (bifenthrin 2.46 μg kg(-1)) in only one of analysed samples, while others were below detection limits. Obtained results indicate that crops from examined agricultural areas are unpolluted by contaminants used for plant protection and nutrition, indicating good agricultural practice regarding pesticides and fertilizer usage as well as moderate industrial production within examined areas.

Identification of amino acid residues involved in substrate specificity of plant acyl-ACP thioesterases using a bioinformatics-guided approach

PubMed Central

Mayer, Kimberly M; Shanklin, John

2007-01-01

Background The large amount of available sequence information for the plant acyl-ACP thioesterases (TEs) made it possible to use a bioinformatics-guided approach to identify amino acid residues involved in substrate specificity. The Conserved Property Difference Locator (CPDL) program allowed the identification of putative specificity-determining residues that differ between the FatA and FatB TE classes. Six of the FatA residue differences identified by CPDL were incorporated into the FatB-like parent via site-directed mutagenesis and the effect of each on TE activity was determined. Variants were expressed in E. coli strain K27 that allows determination of enzyme activity by GCMS analysis of fatty acids released into the medium. Results Substitutions at four of the positions (74, 86, 141, and 174) changed substrate specificity to varying degrees while changes at the remaining two positions, 110 and 221, essentially inactivated the thioesterase. The effects of substitutions at positions 74, 141, and 174 (3-MUT) or 74, 86, 141, 174 (4-MUT) were not additive with respect to specificity. Conclusion Four of six putative specificity determining positions in plant TEs, identified with the use of CPDL, were validated experimentally; a novel colorimetric screen that discriminates between active and inactive TEs is also presented. PMID:17201914

Fluid Physics in a Fluctuating Acceleration Environment

NASA Technical Reports Server (NTRS)

Thomson, J. Ross; Drolet, Francois; Vinals, Jorge

1996-01-01

We summarize several aspects of an ongoing investigation of the effects that stochastic residual accelerations (g-jitter) onboard spacecraft can have on experiments conducted in a microgravity environment. The residual acceleration field is modeled as a narrow band noise, characterized by three independent parameters: intensity (g(exp 2)), dominant angular frequency Omega, and characteristic correlation time tau. Realistic values for these parameters are obtained from an analysis of acceleration data corresponding to the SL-J mission, as recorded by the SAMS instruments. We then use the model to address the random motion of a solid particle suspended in an incompressible fluid subjected to such random accelerations. As an extension, the effect of jitter on coarsening of a solid-liquid mixture is briefly discussed, and corrections to diffusion controlled coarsening evaluated. We conclude that jitter will not be significant in the experiment 'Coarsening of solid-liquid mixtures' to be conducted in microgravity. Finally, modifications to the location of onset of instability in systems driven by a random force are discussed by extending the standard reduction to the center manifold to the stochastic case. Results pertaining to time-modulated oscillatory convection are briefly discussed.

Analysis of Residual Acceleration Effects on Transport and Segregation During Directional Solidification of Tin-Bismuth in the MEPHISTO Furnace Facility

NASA Technical Reports Server (NTRS)

Alexander J. Iwan D. (Principal Investigator)

1996-01-01

The objective of this work is to approach the problem of determining the transport conditions (and effects of residual acceleration) during the plane-front directional solidification of a tin-bismuth alloy under low gravity conditions. The work involves using a combination of 2- and 3-D numerical models, scaling analyses, ID models and the results of ground-based and low-gravity experiments. The latter are to be conducted during the MEPHISTO experiment scheduled for USMP-3 in early 1996. The models will be used to predict the response of the transport conditions and consequent solute segregation in directionally solidifying tin-bismuth melt. Real-time Seebeck voltage variations across a Sn-Bi melt during directional solidification in MEPHISTO on USMP-1 show a distinct variation which can be correlated with thruster firings. The Seebeck voltage measurement is related to the response of the instantaneous average melt composition at the melt-solid interface. This allows a direct comparison of numerical simulations with the Seebeck signals obtained on USMP-1. The effects of such accelerations on composition for a directionally solidifying Sn-Bi alloy have been simulated numerically. USMP-1 acceleration data was used to assist in our choice of acceleration magnitude and orientation. The results show good agreement with experimental observations. The USMP-3 experiments took place earlier this year (February 22 through March 6). There were several differences between the USMP-3 experiments as compared to USMP-1. Firstly a more concentrated alloy was solidified and, secondly, Primary Reaction Control System thruster burns were requested at particular times during four separate growth runs. This allowed us to monitor the response Seebeck response under well-characterized growth conditions. In addition, we carried out simulations during the experiment in order to interpret the Seebeck signal. Preliminary results are described here.

Microwave emission and crop residues

NASA Technical Reports Server (NTRS)

Jackson, Thomas J.; O'Neill, Peggy E.

1991-01-01

A series of controlled experiments were conducted to determine the significance of crop residues or stubble in estimating the emission of the underlying soil. Observations using truck-mounted L and C band passive microwave radiometers showed that for dry wheat and soybeans the dry residue caused negligible attenuation of the background emission. Green residues, with water contents typical of standing crops, did have a significant effect on the background emission. Results for these green residues also indicated that extremes in plant structure, as created using parallel and perpendicular stalk orientations, can cause very large differences in the degree of attenuation.

Reduction of neonicotinoid insecticide residues in Prairie wetlands by common wetland plants.

PubMed

Main, Anson R; Fehr, Jessica; Liber, Karsten; Headley, John V; Peru, Kerry M; Morrissey, Christy A

2017-02-01

Neonicotinoid insecticides are frequently detected in wetlands during the early to mid-growing period of the Canadian Prairie cropping season. These detections also overlap with the growth of macrophytes that commonly surround agricultural wetlands which we hypothesized may reduce neonicotinoid transport and retention in wetlands. We sampled 20 agricultural wetlands and 11 macrophyte species in central Saskatchewan, Canada, over eight weeks to investigate whether macrophytes were capable of reducing movement of neonicotinoids from cultivated fields and/or reducing concentrations in surface water by accumulating insecticide residues into their tissues. Study wetlands were surrounded by clothianidin-treated canola and selected based on the presence (n=10) or absence (n=10) of a zonal plant community. Neonicotinoids were positively detected in 43% of wetland plants, and quantified in 8% of all plant tissues sampled. Three plant species showed high rates of detection: 78% Equisetum arvense (clothianidin, range: plant height, water depth) on neonicotinoid concentrations in Prairie wetlands over time using linear mixed-effects models. Clothianidin concentrations were significantly lower in wetlands surrounded by taller plants (β±S.E.: -0.57±0.12, P≤0.001). The results of this study suggest that macrophytes can play an important role in mitigating water contamination by accumulating neonicotinoids and possibly slowing transport to wetlands during the growing season. Copyright © 2016 Elsevier B

Rapid evolution accelerates plant population spread in fragmented experimental landscapes.

PubMed

Williams, Jennifer L; Kendall, Bruce E; Levine, Jonathan M

2016-07-29

Predicting the speed of biological invasions and native species migrations requires an understanding of the ecological and evolutionary dynamics of spreading populations. Theory predicts that evolution can accelerate species' spread velocity, but how landscape patchiness--an important control over traits under selection--influences this process is unknown. We manipulated the response to selection in populations of a model plant species spreading through replicated experimental landscapes of varying patchiness. After six generations of change, evolving populations spread 11% farther than nonevolving populations in continuously favorable landscapes and 200% farther in the most fragmented landscapes. The greater effect of evolution on spread in patchier landscapes was consistent with the evolution of dispersal and competitive ability. Accounting for evolutionary change may be critical when predicting the velocity of range expansions. Copyright © 2016, American Association for the Advancement of Science.

Removal of residual contamination from clean protective clothing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hylko, J.M.; Miller, M.L.; Brehm, L.E.

This paper reports that during 1986, a small but significant number of skin contamination incidents at Northern States Power Company's Monticello Nuclear Generating Plant were believed to have been caused by residual contamination in dry-cleaned protective clothing. A survey of 24 other nuclear power plants in May and June of 1986 indicated that about half of these facilities had experienced instances of sweat-induced skin contamination on persons wearing laundered, clean protective clothing, and a simple experiment showed that perspiration was indeed capable of removing residual contamination from the 100% cotton fabric used in the Monticello Plant's coveralls. In order tomore » further study this problem, a series of tests were performed on samples of the plant's protective clothing coveralls to determine how effective wet washing methods would be in removing residual contamination from this clothing, which previously had been laundered exclusively by dry cleaning.« less

Characterization and Elimination of Undesirable Protein Residues in Plant Cell Wall Materials for Enhancing Lignin Analysis by Solution-State Nuclear Magnetic Resonance Spectroscopy

DOE PAGES

Kim, Hoon; Padmakshan, Dharshana; Li, Yanding; ...

2017-10-24

Protein polymers exist in every plant cell wall preparation, and they interfere with lignin characterization and quantification. Here, we report the structural characterization of the residual protein peaks in 2D NMR spectra in corn cob and kenaf samples and note that aromatic amino acids are ubiquitous and evident in spectra from various other plants and tissues. The aromatic correlations from amino acid residues were identified and assigned as phenylalanine and tyrosine. Phenylalanine’s 3/5 correlation peak is superimposed on the peak from typical lignin p-hydroxyphenyl (H-unit) structures, causing an overestimation of the H units. Protein contamination also occurs when using cellulasesmore » to prepare enzyme lignins from virtually protein-free wood samples. As a result, we used a protease to remove the protein residues from the ball-milled cell walls, and we were able to reveal H-unit structures in lignins more clearly in the 2D NMR spectra, providing a better basis for their estimation.« less

Characterization and Elimination of Undesirable Protein Residues in Plant Cell Wall Materials for Enhancing Lignin Analysis by Solution-State Nuclear Magnetic Resonance Spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kim, Hoon; Padmakshan, Dharshana; Li, Yanding

Protein polymers exist in every plant cell wall preparation, and they interfere with lignin characterization and quantification. Here, we report the structural characterization of the residual protein peaks in 2D NMR spectra in corn cob and kenaf samples and note that aromatic amino acids are ubiquitous and evident in spectra from various other plants and tissues. The aromatic correlations from amino acid residues were identified and assigned as phenylalanine and tyrosine. Phenylalanine’s 3/5 correlation peak is superimposed on the peak from typical lignin p-hydroxyphenyl (H-unit) structures, causing an overestimation of the H units. Protein contamination also occurs when using cellulasesmore » to prepare enzyme lignins from virtually protein-free wood samples. As a result, we used a protease to remove the protein residues from the ball-milled cell walls, and we were able to reveal H-unit structures in lignins more clearly in the 2D NMR spectra, providing a better basis for their estimation.« less

Acceleration of exotic plant invasion in a forested ecosystem by a generalist herbivore.

PubMed

Eschtruth, Anne K; Battles, John J

2009-04-01

The successful invasion of exotic plants is often attributed to the absence of coevolved enemies in the introduced range (i.e., the enemy release hypothesis). Nevertheless, several components of this hypothesis, including the role of generalist herbivores, remain relatively unexplored. We used repeated censuses of exclosures and paired controls to investigate the role of a generalist herbivore, white-tailed deer (Odocoileus virginianus), in the invasion of 3 exotic plant species (Microstegium vimineum, Alliaria petiolata, and Berberis thunbergii) in eastern hemlock (Tsuga canadensis) forests in New Jersey and Pennsylvania (U.S.A.). This work was conducted in 10 eastern hemlock (T. canadensis) forests that spanned gradients in deer density and in the severity of canopy disturbance caused by an introduced insect pest, the hemlock woolly adelgid (Adelges tsugae). We used maximum likelihood estimation and information theoretics to quantify the strength of evidence for alternative models of the influence of deer density and its interaction with the severity of canopy disturbance on exotic plant abundance. Our results were consistent with the enemy release hypothesis in that exotic plants gained a competitive advantage in the presence of generalist herbivores in the introduced range. The abundance of all 3 exotic plants increased significantly more in the control plots than in the paired exclosures. For all species, the inclusion of canopy disturbance parameters resulted in models with substantially greater support than the deer density only models. Our results suggest that white-tailed deer herbivory can accelerate the invasion of exotic plants and that canopy disturbance can interact with herbivory to magnify the impact. In addition, our results provide compelling evidence of nonlinear relationships between deer density and the impact of herbivory on exotic species abundance. These findings highlight the important role of herbivore density in determining impacts on

Cross-Resistance to Short Residual Sulfonylurea Herbicides in Transgenic Tobacco Plants 1

PubMed Central

Gabard, Jerome M.; Charest, Pierre J.; Iyer, V. N.; Miki, Brian L.

1989-01-01

Transgenic Nicotiana tabacum plants, produced by Agrobacterium tumefaciens-mediated transformation with a mutant gene (csr1-1) coding for acetohydroxyacid synthase (AHAS) from a chlorsulfuron resistant Arabidopsis thaliana line GH50 (GW Haughn et al. [1988] Mol Gen Genet 211: 266-271; GW Haughn, C Somerville [1986] Mol Gen Genet 204: 430-434), were selected directly on 80 micrograms per liter (225 nanomolar) chlorsulfuron. The expression of csr-1 in two separate transgenic lines CHL-1 and CHL-2 was confirmed by biochemical and genetic analyses. The AHAS activity of GH50 and the equivalent component of AHAS activity in CHL-2 was resistant to three short residual sulfonylurea herbicides, DPX-M6316, DPX-A7881, and DPX-L5300, in addition to chlorsulfuron but not to the sulfonylurea CGA 131′036. Cross-resistance to the imidazolinones AC 263, 499, AC 252, 214, and AC 243,997 was not observed. Parallel observations were made on the inhibition of seedling growth in soil or on culture medium. The relevance of these findings for the application of transgenic plants in agriculture is discussed. Images Figure 1 PMID:16667071

Processing hardwood bark residues by screening

Treesearch

David M. Emanuel

1978-01-01

Most of the hardwood bark residues removed by floating-cutterhead or rosserhead debarkers can be processed into acceptable bark products by screening alone. And by prescreening bark residues, operators of bark processing plants can use smaller hammermills than otherwise are required, thus lowering investment and energy costs.

Oxidation of methionine residues: the missing link between stress and signalling responses in plants.

PubMed

Emes, Michael J

2009-08-13

In response to biotic and abiotic stresses, plants induce a complex array of pathways and protein phosphorylation cascades which generally lead to a response aimed at mitigating the particular insult. In many cases, H2O2 has been implicated as the signalling molecule, but, although progress has been made in assembling the downstream components of these signalling pathways, far less is known about the mechanism by which the signal is perceived. In this issue of the Biochemical Journal, Hardin et al. provide evidence for a plausible mechanism by which plants perceive H2O2. Evidence is presented for chemical oxidation of methionine residues by H2O2 at critical hydrophobic positions within the canonical motifs that define the phosphorylation sites of a number of enzymes, thus inhibiting binding of protein kinases. This process is reversible by MSR (methionine sulfoxide reductase) activity in vivo. Using synthetic peptides for a number of enzymes which are phosphorylated by families of protein kinases, including the CDPK (calcium-dependent protein kinase) and AMPK (AMP-activated protein kinase) families, coupled with in vivo studies of assimilatory plant nitrate reductase, the authors demonstrate that this mechanism regulates the ability of kinases to bind the target protein, directly linking oxidative signals to changes in protein phosphorylation. These results may have widespread implications for the perception of redox signalling in plants and animals.

Global impact of accelerated plant breeding: Evidence from a meta-analysis on rice breeding.

PubMed

Lenaerts, Bert; de Mey, Yann; Demont, Matty

2018-01-01

Rice breeders in Asia and elsewhere in the world have long overlooked trying to shorten the time it takes to develop new varieties. Plant breeders have proposed a technique called Rapid Generation Advance (RGA) as a way to accelerate the results of public rice breeding programs. However, little is known about RGA's potential impact. Here, we present the first results of a global impact study of RGA. More specifically, we calculated the multiplicator effects of RGA on the research benefits generated by conventional rice breeding programs and applied them to a meta-analysis of selected impact studies in the literature. These insights are a first crucial step in developing a targeted approach for disseminating RGA technology among rice breeders to accelerate the impact of their public rice breeding programs around the world. We show that the additional benefits due to time savings are considerable and offer some insights into the economics of breeding. Our results confirm that the adoption of accelerated breeding would lead to substantial advantages to rice breeding programs and the earlier variety release leads to significant economic benefits to society. This can be important to policy makers when reshaping their public breeding methods and optimising their return on research investments in breeding.

The effects of composting on the nutritional composition of fibrous bio-regenerative life support systems (BLSS) plant waste residues and its impact on the growth of Nile tilapia ( Oreochromis niloticus)

NASA Astrophysics Data System (ADS)

Gonzales, John M.; Lowry, Brett A.; Brown, Paul B.; Beyl, Caula A.; Nyochemberg, Leopold

2009-04-01

Utilization of bio-regenerative life support systems (BLSS) plant waste residues as a nutritional source by Nile tilapia ( Oreochromis niloticus) has proven problematic as a result of high concentrations of fibrous compounds in the plant waste residues. Nutritional improvement of plant waste residues by composting with the oyster mushroom ( Pleurotus ostreatus), and the effects on growth and nutrient utilization of Nile tilapia fed such residues were evaluated. Five Nile tilapia (mean weight = 70.9 ± 3.1 g) were stocked in triplicate aquaria and fed one of two experimental diets, cowpea (CP) and composted cowpea (CCP), twice daily for a period of 8 weeks. Composting of cowpea residue resulted in reduced concentrations of nitrogen-free extract, hemi-cellulose and trypsin inhibitor activity, though trypsin inhibitor activity remained high. Composting did not reduce crude fiber, lignin, or cellulose concentrations in the diet. No significant differences ( P < 0.05) were observed in weight gain, specific growth rate, survival rate, daily consumption, and food conversion ratio between tilapia fed CP and CCP. These results suggest that P. ostreatus is not a suitable candidate for culture in conjunction with the culture of Nile tilapia. Additional work is needed to determine what, if any, benefit can be obtained from incorporating composted residue as feed for Nile tilapia.

The effects of Pantoea sp. strain Y4-4 on alfalfa in the remediation of heavy-metal-contaminated soil, and auxiliary impacts of plant residues on the remediation of saline-alkali soils.

PubMed

Li, Shuhuan; Wang, Jie; Gao, Nanxiong; Liu, Lizhu; Chen, Yahua

2017-04-01

The plant-growth-promoting rhizobacterium (PGPR) Y4-4 was isolated from plant rhizosphere soil and identified as Pantoea sp. by 16S rRNA sequence analysis. The effects of strain Y4-4 on alfalfa grown in heavy-metals-contaminated soil was investigated using a pot experiment. In a Cu-rich environment, the shoot dry mass and total dry mass of plants inoculated with strain Y4-4 increased by 22.6% and 21%, and Cu accumulation increased by 15%. In a Pb-Zn-rich environment, the shoot dry mass and total dry mass of plants inoculated with strain Y4-4 increased by 23.4% and 22%, and Zn accumulation increased by 30.3%. In addition, the salt tolerance and biomass of wheat seedlings could be improved by applying strain Y4-4 mixed with plant residue as a result of the Cu-rich plant residues providing copper nutrition to wheat. This study offers an efficient PGPR with strong salt tolerance and a safe strategy for the post-treatment of plant residue.

Decomposition of PCBs in transformer oil using an electron beam accelerator

NASA Astrophysics Data System (ADS)

Jung, In-Ha; Lee, Myun-Joo; Mah, Yoon-Jung

2012-07-01

Decomposition of PCBs in commercially used transformer oil used for more than 30 years has been carried out at normal temperature and pressure without any additives using an electron beam accelerator. The experiments were carried out in two ways: batch and continuous pilot plant with 1.5 MeV of energy, a 50 mA current, and 75 kW of power in a commercial scale accelerator. The electron beam irradiation seemed to transform large molecular weight compounds into lower ones, but the impact was considered too small on the physical properties of oil. Residual concentrations of PCBs after irradiation depend on the absorption dose of the electron beam energy, but aliphatic chloride compounds were produced at higher doses of irradiation. As the results from FT-NMR, chloride ions decomposed from the PCBs are likely to react with aliphatic hydro carbon compounds rather than existing as free radical ions in the transformer oil. Since this is a dry process, treated oil can be used as cutting oil or machine oil for heavy equipment without any additional treatments.

Effects of normal acceleration on transient burning rate augmentation of an aluminized solid propellant

NASA Technical Reports Server (NTRS)

Northam, G. B.

1972-01-01

Instantaneous burning rate data for a polybutadiene acrylic acid propellant, containing 16 weight percent aluminum, were calculated from the pressure histories of a test motor with 96.77 sq cm of burning area and a 5.08-cm-thick propellant web. Additional acceleration tests were conducted with reduced propellant web thicknesses of 3.81, 2.54, and 1.27 cm. The metallic residue collected from the various web thickness tests was characterized by weight and shape and correlated with the instantaneous burning rate measurements. Rapid depressurization extinction tests were conducted in order that surface pitting characteristics due to localized increased burning rate could be correlated with the residue analysis and the instantaneous burning rate data. The acceleration-induced burning rate augmentation was strongly dependent on propellant distance burned, or burning time, and thus was transient in nature. The results from the extinction tests and the residue analyses indicate that the transient rate augmentation was highly dependent on local enhancement of the combustion zone heat feedback to the surface by the growth of molten residue particles on or just above the burning surface. The size, shape, and number density of molten residue particles, rather than the total residue weight, determined the acceleration-induced burning rate augmentation.

[Plant protection products and their residues : Aspects of consumer safety in context of the new EU regulations].

PubMed

Banasiak, U; Michalski, B; Pfeil, R; Solecki, R

2010-06-01

The law regulating plant protection products (PPP) in the European Union (EU) was fundamentally revised through the introduction of Regulation (EC) No. 1107/2009 which is due to enter into force on 14 June 2011. EU-wide harmonized maximum residue levels (MRLs) for the active substances of PPP in foods are laid down in Regulation (EC) No. 396/2005 and apply since entry into force of the regulation on 1 September 2008. The goal of both regulations is to strengthen the level of consumer protection. PPP are subject to a strict assessment of active substances, which is regulated at the EU level as well as an authorization procedure in the EU Member States. Prior to application for authorization of a PPP, the active substance(s) it contains must be included in a positive list. Tests regarding the toxicity and residue behavior of PPP must be conducted by the applicant, and the respective documents must be submitted to the authorities for evaluation. Following review of the required data, toxicological threshold values are derived, consumer exposure is assessed, and the risk to health is evaluated. The goal of this evaluation is to ensure that the use of PPP according to good plant protection practice does not have any harmful effects on human health.

[Migrants from disposable gloves and residual acrylonitrile].

PubMed

Wakui, C; Kawamura, Y; Maitani, T

2001-10-01

Disposable gloves made from polyvinyl chloride with and without di(2-ethylhexyl) phthalate (PVC-DEHP, PVC-NP), polyethylene (PE), natural rubber (NR) and nitrile-butadiene rubber (NBR) were investigated with respect to evaporation residue, migrated metals, migrants and residual acrylonitrile. The evaporation residue found in n-heptane was 870-1,300 ppm from PVC-DEHP and PVC-NP, which was due to the plasticizers. Most of the PE gloves had low evaporation residue levels and migrants, except for the glove designated as antibacterial, which released copper and zinc into 4% acetic acid. For the NR and NBR gloves, the evaporation residue found in 4% acetic acid was 29-180 ppm. They also released over 10 ppm of calcium and 6 ppm of zinc into 4% acetic acid, and 1.68-8.37 ppm of zinc di-ethyldithiocarbamate and zinc di-n-butyldithiocarbamate used as vulcanization accelerators into n-heptane. The acrylonitrile content was 0.40-0.94 ppm in NBR gloves.

Gyrokinetic theory of turbulent acceleration and momentum conservation in tokamak plasmas

NASA Astrophysics Data System (ADS)

Lu, WANG; Shuitao, PENG; P, H. DIAMOND

2018-07-01

Understanding the generation of intrinsic rotation in tokamak plasmas is crucial for future fusion reactors such as ITER. We proposed a new mechanism named turbulent acceleration for the origin of the intrinsic parallel rotation based on gyrokinetic theory. The turbulent acceleration acts as a local source or sink of parallel rotation, i.e., volume force, which is different from the divergence of residual stress, i.e., surface force. However, the order of magnitude of turbulent acceleration can be comparable to that of the divergence of residual stress for electrostatic ion temperature gradient (ITG) turbulence. A possible theoretical explanation for the experimental observation of electron cyclotron heating induced decrease of co-current rotation was also proposed via comparison between the turbulent acceleration driven by ITG turbulence and that driven by collisionless trapped electron mode turbulence. We also extended this theory to electromagnetic ITG turbulence and investigated the electromagnetic effects on intrinsic parallel rotation drive. Finally, we demonstrated that the presence of turbulent acceleration does not conflict with momentum conservation.

Trends for Electron Beam Accelerator Applications in Industry

NASA Astrophysics Data System (ADS)

Machi, Sueo

2011-02-01

Electron beam (EB) accelerators are major pieces of industrial equipment used for many commercial radiation processing applications. The industrial use of EB accelerators has a history of more than 50 years and is still growing in terms of both its economic scale and new applications. Major applications involve the modification of polymeric materials to create value-added products, such as heat-resistant wires, heat-shrinkable sheets, automobile tires, foamed plastics, battery separators and hydrogel wound dressing. The surface curing of coatings and printing inks is a growing application for low energy electron accelerators, resulting in an environmentally friendly and an energy-saving process. Recently there has been the acceptance of the use of EB accelerators in lieu of the radioactive isotope cobalt-60 as a source for sterilizing disposable medical products. Environmental protection by the use of EB accelerators is a new and important field of application. A commercial plant for the cleaning flue gases from a coal-burning power plant is in operation in Poland, employing high power EB accelerators. In Korea, a commercial plant uses EB to clean waste water from a dye factory.

Long-pulse beam acceleration of MeV-class H(-) ion beams for ITER NB accelerator.

PubMed

Umeda, N; Kashiwagi, M; Taniguchi, M; Tobari, H; Watanabe, K; Dairaku, M; Yamanaka, H; Inoue, T; Kojima, A; Hanada, M

2014-02-01

In order to realize neutral beam systems in International Thermonuclear Experimental Reactor whose target is to produce a 1 MeV, 200 A/m(2) during 3600 s D(-) ion beam, the electrostatic five-stages negative ion accelerator so-called "MeV accelerator" has been developed at Japan Atomic Energy Agency. To extend pulse length, heat load of the acceleration grids was reduced by controlling the ion beam trajectory. Namely, the beam deflection due to the residual magnetic field of filter magnet was suppressed with the newly developed extractor with a 0.5 mm off-set aperture displacement. The new extractor improved the deflection angle from 6 mrad to 1 mrad, resulting in the reduction of direct interception of negative ions from 23% to 15% of the total acceleration power, respectively. As a result, the pulse length of 130 A/m(2), 881 keV H(-) ion beam has been successfully extended from a previous value of 0.4 s to 8.7 s. This is the first long pulse negative ion beam acceleration over 100 MW/m(2).

Fluorescence of crop residue: postmortem analysis of crop conditions

NASA Astrophysics Data System (ADS)

McMurtrey, James E., III; Kim, Moon S.; Daughtry, Craig S. T.; Corp, Lawrence A.; Chappelle, Emmett W.

1997-07-01

Fluorescence of crop residues at the end of the growing season may provide an indicator of the past crop's vegetative condition. Different levels of nitrogen (N) fertilization were applied to field grown corn and wheat at Beltsville, Maryland. The N fertilizer treatments produce a range of physiological conditions, pigment concentrations, biomass levels, and grain yields that resulted in varying growth and stress conditions in the living crops. After normal harvesting procedures the crop residues remained. The fluorescence spectral characteristics of the plant residues from crops grown under different levels of N nutrition were analyzed. The blue-green fluorescence response of in-vitro residue biomass of the N treated field corn had different magnitudes. A blue-green- yellow algorithm, (460/525)*600 nm, gave the best separations between prior corn growth conditions at different N fertilization levels. Relationships between total dry biomass, the grain yield, and fluorescence properties in the 400 - 670 nm region of the spectrum were found in both corn and wheat residues. The wheat residue was analyzed to evaluate the constituents responsible for fluorescence. A ratio of the blue-green, 450/550 nm, images gave the best separation among wheat residues at different N fertilization levels. Fluorescence of extracts from wheat residues showed inverse fluorescence intensities as a function of N treatments compared to that of the intact wheat residue or ground residue samples. The extracts also had an additional fluorescence emission peak in the red, 670 nm. Single band fluorescence intensity in corn and wheat residues is due mostly to the quantity of the material on the soil surface. Ratios of fluorescence bands varied as a result of the growth conditions created by the N treatments and are thought to be indicative of the varying concentrations of the plant residues fluorescing constituents. Estimates of the amount and cost effectiveness of N fertilizers to satisfy

Redox regulation of plant S-nitrosoglutathione reductase activity through post-translational modifications of cysteine residues.

PubMed

Tichá, Tereza; Lochman, Jan; Činčalová, Lucie; Luhová, Lenka; Petřivalský, Marek

2017-12-09

Nitric oxide (NO) is considered as a signalling molecule involved in a variety of important physiological and pathological processes in plant and animal systems. The major pathway of NO reactions in vivo represents S-nitrosation of thiols to form S-nitrosothiols. S-nitrosoglutathione reductase (GSNOR) is the key enzyme in the degradation pathway of S-nitrosoglutathione (GSNO), a low-molecular weight adduct of NO and glutathione. GSNOR indirectly regulates the level of protein S-nitrosothiol in the cells. This study was focused on the dynamic regulation of the activity of plant GSNORs through reversible S-nitrosation and/or oxidative modifications of target cysteine residues. Pre-incubation with NO/NO - donors or hydrogen peroxide resulted in a decreased reductase and dehydrogenase activity of all studied plant GSNORs. Incubation with thiol reducing agent completely reversed inhibitory effects of nitrosative modifications and partially also oxidative inhibition. In biotin-labelled samples, S-nitrosation of plant GSNORs was confirmed after immunodetection and using mass spectrometry S-nitrosation of conserved Cys271 was identified in tomato GSNOR. Negative regulation of constitutive GSNOR activity in vivo by nitrosative or oxidative modifications might present an important mechanism to control GSNO levels, a critical mediator of the downstream signalling effects of NO, as well as for formaldehyde detoxification in dehydrogenase reaction mode. Copyright © 2017. Published by Elsevier Inc.

Industrial demonstration plant for the gasification of herb residue by fluidized bed two-stage process.

PubMed

Zeng, Xi; Shao, Ruyi; Wang, Fang; Dong, Pengwei; Yu, Jian; Xu, Guangwen

2016-04-01

A fluidized bed two-stage gasification process, consisting of a fluidized-bed (FB) pyrolyzer and a transport fluidized bed (TFB) gasifier, has been proposed to gasify biomass for fuel gas production with low tar content. On the basis of our previous fundamental study, an autothermal two-stage gasifier has been designed and built for gasify a kind of Chinese herb residue with a treating capacity of 600 kg/h. The testing data in the operational stable stage of the industrial demonstration plant showed that when keeping the reaction temperatures of pyrolyzer and gasifier respectively at about 700 °C and 850 °C, the heating value of fuel gas can reach 1200 kcal/Nm(3), and the tar content in the produced fuel gas was about 0.4 g/Nm(3). The results from this pilot industrial demonstration plant fully verified the feasibility and technical features of the proposed FB two-stage gasification process. Copyright © 2016. Published by Elsevier Ltd.

Allelopathic impact of HoCP 96-540 field residue on seed germination

USDA-ARS?s Scientific Manuscript database

Research indicates that sugarcane field residue and sugarcane mill bagasse may be allelopathic. Allelopathy is the chemical interaction between plants, which may result in the inhibition of plant growth and development. Previous research in Louisiana indicated that sugarcane field residue may inhibi...

Effect of Pretreatment on Biomass Residue Structure and the Application of Pyrolysed and Composted Biomass Residues in Soilless Culture

PubMed Central

Suo, Linna; Sun, Xiangyang; Jiang, Weijie

2013-01-01

The changes in the structural characteristics of biomass residues during pyrolysis and composting were investigated. The biomass residues particles were prepared by pyrolysing at temperatures ranging from 350 to 400. For soilless production of the ornamental plant Anthurium andraeanum, pure sphagnum peat moss (P) has traditionally been used as the growing medium. This use of P must be reduced, however, because P is an expensive and nonrenewable resource. The current study investigated the use of biomass residues as substitutes for P in A. andraeanum production. Plants were grown for 15 months in 10 soilless media that contained different proportions of pyrolysed corn cobs (PC), composted corn cobs (C), pyrolysed garden wastes (PG), and P. Although the media altered the plant nutrient content, A. andraeanum growth, development, and yield were similar with media consisting of 50% P+50% PC, 50% P+35% PC+15% PG, and 100% P. This finding indicates that, when pyrolysed, organic wastes, which are otherwise an environmental problem, can be used to reduce the requirement for peat in the soilless culture of A. andraeanum. PMID:23704995

Accelerated coffee pulp composting.

PubMed

Sánchez, G; Olguín, E J; Mercado, G

1999-02-01

The effect of two abundant, easily available and very low-cost agro-industrial organic residues, i.e., filter cake from the sugar industry and poultry litter, on the composting stabilization time of coffee pulp and on the quality of the produced compost, was evaluated. Piles of one cubic meter were built and monitored within the facilities of a coffee processing plant in the Coatepec region of the State of Veracruz, Mexico. Manual aeration was carried out once a week. A longer thermophilic period (28 days) and a much lower C/N ratio (in the range of 6.9-9.1) were observed in the piles containing the amendments, as compared to the control pile containing only coffee pulp (14 days and a C/N ratio of 14.4, respectively). The maximum assimilation rate of the reducing sugars was 1.6 g kg-1 d-1 (from 7.5 to 5.3%) during the first two weeks when accelerators were present in the proportion of 20% filter cake plus 20% poultry litter, while they accumulated at a rate of 1.2 g kg-1 d-1 (from 7.4 to 9.13%) during the same period in the control pile. The best combination of amendments was 30% filter cake with 20% poultry litter, resulting in a final nitrogen content as high as 4.81%. The second best combination was 20% filter cake with 10% poultry litter, resulting in a compost which also contained a high level of total nitrogen (4.54%). It was concluded that the use of these two residues enhanced the composting process of coffee pulp, promoting a shorter stabilization period and yielding a higher quality of compost.

27 CFR 20.215 - Shipment of articles and spirits residues for redistillation.

Code of Federal Regulations, 2010 CFR

2010-04-01

... AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS DISTRIBUTION AND USE OF DENATURED... articles and spirits residues for redistillation. (a) The proprietor of a distilled spirits plant... distilled spirits plant for redistillation shall— (1) Identify each package or articles or spirits residues...

40 CFR 180.155 - 1-Naphthaleneacetic acid; tolerances for residues.

Code of Federal Regulations, 2011 CFR

2011-07-01

... (CONTINUED) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific... established for the combined residues of the plant growth regulator 1-naphthaleneacetic acid and its...

40 CFR 180.155 - 1-Naphthaleneacetic acid; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

... (CONTINUED) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific... established for the combined residues of the plant growth regulator 1-naphthaleneacetic acid and its...

40 CFR 180.202 - p-Chlorophenoxyacetic acid; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

... (CONTINUED) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific... established for the combined residues of the plant regulator p-chlorophenoxyacetic acid and its metabolite p...

40 CFR 180.202 - p-Chlorophenoxyacetic acid; tolerances for residues.

Code of Federal Regulations, 2011 CFR

2011-07-01

... (CONTINUED) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL RESIDUES IN FOOD Specific... established for the combined residues of the plant regulator p-chlorophenoxyacetic acid and its metabolite p...

Radiobiological effectiveness of laser accelerated electrons in comparison to electron beams from a conventional linear accelerator.

PubMed

Laschinsky, Lydia; Baumann, Michael; Beyreuther, Elke; Enghardt, Wolfgang; Kaluza, Malte; Karsch, Leonhard; Lessmann, Elisabeth; Naumburger, Doreen; Nicolai, Maria; Richter, Christian; Sauerbrey, Roland; Schlenvoigt, Hans-Peter; Pawelke, Jörg

2012-01-01

The notable progress in laser particle acceleration technology promises potential medical application in cancer therapy through compact and cost effective laser devices that are suitable for already existing clinics. Previously, consequences on the radiobiological response by laser driven particle beams characterised by an ultra high peak dose rate have to be investigated. Therefore, tumour and non-malignant cells were irradiated with pulsed laser accelerated electrons at the JETI facility for the comparison with continuous electrons of a conventional therapy LINAC. Dose response curves were measured for the biological endpoints clonogenic survival and residual DNA double strand breaks. The overall results show no significant differences in radiobiological response for in vitro cell experiments between laser accelerated pulsed and clinical used electron beams. These first systematic in vitro cell response studies with precise dosimetry to laser driven electron beams represent a first step toward the long term aim of the application of laser accelerated particles in radiotherapy.

GRACE gravity field recovery using refined acceleration approach

NASA Astrophysics Data System (ADS)

Li, Zhao; van Dam, Tonie; Weigelt, Matthias

2017-04-01

Since 2002, the GRACE mission has yielded monthly gravity field solutions with such a high level of quality that we have been able to observe so many changes to the Earth mass system. Based on GRACE L1B observations, a number of official monthly gravity field models have been developed and published using different methods, e.g. the CSR RL05, JPL RL05, and GFZ RL05 are being computed by a dynamic approach, the ITSG and Tongji GRACE are generated using what is known as the short-arc approach, the AIUB models are computed using celestial mechanics approach, and the DMT-1 model is calculated by means of an acceleration approach. Different from the DMT-1 model, which links the gravity field parameters directly to the bias-corrected range measurements at three adjacent epochs, in this work we present an alternative acceleration approach which connects range accelerations and velocity differences to the gradient of the gravitational potential. Due to the fact that GPS derived velocity difference is provided at a lower precision, we must reduce this approach to residual quantities using an a priori gravity field which allows us to subsequently neglect the residual velocity difference term. We find that this assumption would cause a problem in the low-degree gravity field coefficient, particularly for degree 2 and also from degree 16 to 26. To solve this problem, we present a new way of handling the residual velocity difference term, that is to treat this residual velocity difference term as unknown but estimable quantity, as it depends on the unknown residual gravity field parameters and initial conditions. In other word, we regard the kinematic orbit position vectors as pseudo observations, and the corrections of orbits are estimated together with both the geopotential coefficients and the accelerometer scale/bias by using a weighted least square adjustment. The new approach is therefore a refinement of the existing approach but offers a better approximation to reality

Accelerated decay rates drive soil organic matter persistence and storage in temperate forests via greater mineral stabilization of microbial residues.

NASA Astrophysics Data System (ADS)

Phillips, R.; Craig, M.; Turner, B. L.; Liang, C.

2017-12-01

Climate predicts soil organic matter (SOM) stocks at the global scale, yet controls on SOM stocks at finer spatial scales are still debated. A current hypothesis predicts that carbon (C) and nitrogen (N) storage in soils should be greater when decomposition is slow owing to microbial competition for nutrients or the recalcitrance of organic substrates (hereafter the `slow decay' hypothesis). An alternative hypothesis predicts that soil C and N storage should be greater in soils with rapid decomposition, owing to the accelerated production of microbial residues and their stabilization on soil minerals (hereafter the `stabilization hypothesis'). To test these alternative hypotheses, we quantified soil C and N to 1-m depth in temperate forests across the Eastern and Midwestern US that varied in their biotic, climatic, and edaphic properties. At each site, we sampled (1) soils dominated by arbuscular mycorrhizal (AM) tree species, which typically have fast decay rates and accelerated N cycling, (2) soils dominated by ectomycorrhizal (ECM) tree species, which generally have slow decay rates and slow N cycling, and (3) soils supporting both AM and ECM trees. To the extent that trees and theor associated microbes reflect and reinforce soil conditions, support for the slow decay hypothesis would be greater SOM storage in ECM soils, whereas support for the stabilization hypothesis would be greater SOM storage in AM soils. We found support for both hypotheses, as slow decomposition in ECM soils increased C and N storage in topsoil, whereas fast decomposition in AM soils increased C and N storage in subsoil. However, at all sites we found 57% greater total C and N storage in the entire profile in AM- soils (P < 0.0001), supporting the stabilization hypothesis. Amino sugar biomarkers (an indicator of microbial necromass) and particle size fractionation revealed that the greater SOM storage in AM soils was driven by an accumulation of microbial residues on clay minerals and

Residual chromatin breaks as biodosimetry for cell killing by carbon ions.

PubMed

Suzuki, M; Kase, Y; Nakano, T; Kanai, T; Ando, K

1998-01-01

We have studied the relationship between cell killing and the induction of residual chromatin breaks on various human cell lines and primary cultured cells obtained by biopsy from patients irradiated with either X-rays or heavy-ion beams to identify potential bio-marker of radiosensitivity for radiation-induced cell killing. The carbon-ion beams were accelerated with the Heavy Ion Medical Accelerator in Chiba (HIMAC). Six primary cultures obtained by biopsy from 6 patients with carcinoma of the cervix were irradiated with two different mono-LET beams (LET = 13 keV/micrometer, 76 keV/micrometer) and 200kV X rays. Residual chromatin breaks were measured by counting the number of non-rejoining chromatin fragments detected by the premature chromosome condensation (PCC) technique after a 24 hour post-irradiation incubation period. The induction rate of residual chromatin breaks per cell per Gy was the highest for 76 keV/micrometer beams on all of the cells. Our results indicated that cell which was more sensitive to the cell killing was similarly more susceptible to induction of residual chromatin breaks. Furthermore there is a good correlation between these two end points in various cell lines and primary cultured cells. This suggests that the detection of residual chromatin breaks by the PCC technique may be useful as a predictive assay of tumor response to cancer radiotherapy.

Residual chromatin breaks as biodosimetry for cell killing by carbon ions

NASA Astrophysics Data System (ADS)

Suzuki, M.; Kase, Y.; Nakano, T.; Kanai, T.; Ando, K.

1998-11-01

We have studied the relationship between cell killing and the induction of residual chromatin breaks on various human cell lines and primary cultured cells obtained by biopsy from patients irradiated with either X-rays or heavy-ion beams to identify potential bio-marker of radiosensitivity for radiation-induced cell killing. The carbon-ion beams were accelerated with the Heavy Ion Medical Accelerator in Chiba (HIMAC). Six primary cultures obtained by biopsy from 6 patients with carcinoma of the cervix were irradiated with two different mono-LET beams (LET = 13 keV/μm, 76 keV/μm) and 200kV X rays. Residual chromatin breaks were measured by counting the number of non-rejoining chromatin fragments detected by the premature chromosome condensation (PCC) technique after a 24 hour post-irradiation incubation period. The induction rate of residual chromatin breaks per cell per Gy was the highest for 76 keV/μm beams on all of the cells. Our results indicated that cell which was more sensitive to the cell killing was similarly more susceptible to induction of residual chromatin breaks. Furthermore there is a good correlation between these two end points in various cell lines and primary cultured cells. This suggests that the detection of residual chromatin breaks by the PCC technique may be useful as a predictive assay of tumor response to cancer radiotherapy.

Lemna minor tolerance to metal-working fluid residues: implications for rhizoremediation.

PubMed

Grijalbo, L; Becerril, J M; Barrutia, O; Gutierrez-Mañero, J; Lucas Garcia, J A

2016-07-01

For the first time in the literature, duckweed (Lemna minor) tolerance (alone or in combination with a consortium of bacteria) to spent metal-working fluid (MWF) was assessed, together with its capacity to reduce the chemical oxygen demand (COD) of this residue. In a preliminary study, L. minor response to pre-treated MWF residue (ptMWF) and vacuum-distilled MWF water (MWFw) was tested. Plants were able to grow in both residues at different COD levels tested (up to 2300 mg·l(-1) ), showing few toxicity symptoms (mainly growth inhibition). Plant response to MWFw was more regular and dose responsive than when exposed to ptMWF. Moreover, COD reduction was less significant in ptMWF. Thus, based on these preliminary results, a second study was conducted using MWFw to test the effectiveness of inoculation with a bacterial consortium isolated from a membrane bioreactor fed with the same residue. After 5 days of exposure, COD in solutions containing inoculated plants was significantly lower than in non-inoculated ones. Moreover, inoculation reduced β+γ-tocopherol levels in MWFw-exposed plants, suggesting pollutant imposed stress was reduced. We therefore conclude from that L. minor is highly tolerant to spent MWF residues and that this species can be very useful, together with the appropriate bacterial consortium, in reducing COD of this residue under local legislation limits and thus minimise its potential environmental impact. Interestingly, the lipophilic antioxidant tocopherol (especially the sum of β+γ isomers) proved to be an effective plant biomarker of pollution. © 2016 German Botanical Society and The Royal Botanical Society of the Netherlands.

Crop residues for advanced biofuels workshop: A synposis

USDA-ARS?s Scientific Manuscript database

Crop residues are being harvested for a variety of purposes including their use as livestock feed and to produce advanced biofuels. Crop residue harvesting, by definition, reduces the potential annual carbon input to the soil from aboveground biomass but does not affect input from plant roots. The m...

40 CFR 180.293 - Endothall; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 0.2 parts per million is established for residues of the herbicide endothall (7 - oxabicyclo[2.2.1...-dimethylalkylamine, and mono-N-N,-dimethylalkylamine salts as algicides or herbicides to control aquatic plants in.... Tolerances are established for the indirect or inadvertent combined residues of the herbicide, endothall (7...

40 CFR 180.293 - Endothall; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 0.2 parts per million is established for residues of the herbicide endothall (7 - oxabicyclo[2.2.1...-dimethylalkylamine, and mono-N-N,-dimethylalkylamine salts as algicides or herbicides to control aquatic plants in.... Tolerances are established for the indirect or inadvertent combined residues of the herbicide, endothall (7...

Electron acceleration by turbulent plasmoid reconnection

NASA Astrophysics Data System (ADS)

Zhou, X.; Büchner, J.; Widmer, F.; Muñoz, P. A.

2018-04-01

In space and astrophysical plasmas, like in planetary magnetospheres, as that of Mercury, energetic electrons are often found near current sheets, which hint at electron acceleration by magnetic reconnection. Unfortunately, electron acceleration by reconnection is not well understood yet, in particular, acceleration by turbulent plasmoid reconnection. We have investigated electron acceleration by turbulent plasmoid reconnection, described by MHD simulations, via test particle calculations. In order to avoid resolving all relevant turbulence scales down to the dissipation scales, a mean-field turbulence model is used to describe the turbulence of sub-grid scales and their effects via a turbulent electromotive force (EMF). The mean-field model describes the turbulent EMF as a function of the mean values of current density, vorticity, magnetic field as well as of the energy, cross-helicity, and residual helicity of the turbulence. We found that, mainly around X-points of turbulent reconnection, strongly enhanced localized EMFs most efficiently accelerated electrons and caused the formation of power-law spectra. Magnetic-field-aligned EMFs, caused by the turbulence, dominate the electron acceleration process. Scaling the acceleration processes to parameters of the Hermean magnetotail, electron energies up to 60 keV can be reached by turbulent plasmoid reconnection through the thermal plasma.

40 CFR 180.384 - Mepiquat (N,N-dimethylpip-eridinium); tolerances for residues.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) Tolerances are established for residues of the plant growth regulator mepiquat (N,N-dimethylpiperidinium) in... byproducts 6.0 Cotton, undelinted seed 2.0 Goat, meat byproducts 0.1 Hog, meat byproducts 0.1 Horse, meat byproducts 0.1 Sheep, meat byproducts 0.1 (2) Tolerances are established for residues of the plant growth...

40 CFR 180.293 - Endothall; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

... established for residues of the herbicide endothall (7 - oxabicyclo[2.2.1] heptane-2,3-dicarboxylic acid) in...,-dimethylalkylamine salts as algicides or herbicides to control aquatic plants in canals, lakes, ponds, and other... indirect or inadvertent combined residues of the herbicide, endothall (7 - oxabicyclo[2.2.1] heptane-2,3...

A key enzyme of animal steroidogenesis can function in plants enhancing their immunity and accelerating the processes of growth and development.

PubMed

Shpakovski, George V; Spivak, Svetlana G; Berdichevets, Irina N; Babak, Olga G; Kubrak, Svetlana V; Kilchevsky, Alexander V; Aralov, Andrey V; Slovokhotov, Ivan Yu; Shpakovski, Dmitry G; Baranova, Ekaterina N; Khaliluev, Marat R; Shematorova, Elena K

2017-11-14

The initial stage of the biosynthesis of steroid hormones in animals occurs in the mitochondria of steroidogenic tissues, where cytochrome P450 SCC (CYP11A1) encoded by the CYP11A1 gene catalyzes the conversion of cholesterol into pregnenolone - the general precursor of all the steroid hormones, starting with progesterone. This stage is missing in plants where mitochondrial cytochromes P450 (the mito CYP clan) have not been found. Generating transgenic plants with a mitochondrial type P450 from animals would offer an interesting option to verify whether plant mitochondria could serve as another site of P450 monooxygenase reaction for the steroid hormones biosynthesis. For a more detailed comparison of steroidogenic systems of Plantae and Animalia, we have created and studied transgenic tobacco and tomato plants efficiently expressing mammalian CYP11A1 cDNA. The detailed phenotypic characterization of plants obtained has shown that through four generations studied, the transgenic tobacco plants have reduced a period of vegetative development (early flowering and maturation of bolls), enlarged biomass and increased productivity (quantity and quality of seeds) as compared to the only empty-vector containing or wild type plants. Moreover, the CYP11A1 transgenic plants show resistance to such fungal pathogen as Botrytis cinerea. Similar valuable phenotypes (the accelerated course of ontogenesis and/or stress resistance) are also visible in two clearly distinct transgenic tomato lines expressing CYP11A1 cDNA: one line (No. 4) has an accelerated rate of vegetative development, while the other (No. 7) has enhanced immunity to abiotic and biotic stresses. The progesterone level in transgenic tobacco and tomato leaves is 3-5 times higher than in the control plants of the wild type. For the first time, we could show the compatibility in vivo of even the most specific components of the systems of biosynthesis of steroid hormones in Plantae and Animalia. The hypothesis is

Ammonia volatilization from crop residues and frozen green manure crops

NASA Astrophysics Data System (ADS)

de Ruijter, F. J.; Huijsmans, J. F. M.; Rutgers, B.

2010-09-01

Agricultural systems can lose substantial amounts of nitrogen (N). To protect the environment, the European Union (EU) has adopted several directives that set goals to limit N losses. National Emission Ceilings (NEC) are prescribed in the NEC directive for nitrogen oxides and ammonia. Crop residues may contribute to ammonia volatilization, but sufficient information on their contribution to the national ammonia volatilization is lacking. Experiments were carried out with the aim to assess the ammonia volatilization of crop residues left on the soil surface or incorporated into the soil under the conditions met in practice in the Netherlands during late autumn and winter. Ammonia emission from residues of broccoli, leek, sugar beet, cut grass, fodder radish (fresh and frozen) and yellow mustard (frozen) was studied during two winter seasons using volatilization chambers. Residues were either placed on top of soil or mixed with soil. Mixing residues with soil gave insignificant ammonia volatilization, whereas volatilization was 5-16 percent of the N content of residues when placed on top of soil. Ammonia volatilization started after at least 4 days. Total ammonia volatilization was related to C/N-ratio and N concentration of the plant material. After 37 days, cumulative ammonia volatilization was negligible from plant material with N concentration below 2 percent, and was 10 percent of the N content of plant material with 4 percent N. These observations can be explained by decomposition of plant material by micro-organisms. After an initial built up of the microbial population, NH 4+ that is not needed for their own growth is released and can easily emit as NH 3 at the soil surface. The results of the experiments were used to estimate the contribution of crop residues to ammonia volatilization in the Netherlands. Crop residues of arable crops and residues of pasture topping may contribute more than 3 million kg NH 3-N to the national ammonia volatilization of the

Carbon decomposition process of the residual biomass in the paddy soil of a single-crop rice field

NASA Astrophysics Data System (ADS)

Okada, K.; Iwata, T.

2014-12-01

In cultivated fields, residual organic matter is plowed into soil after harvest and decaying in fallow season. Greenhouse gases such as CO2 and CH4 is generated by the decomposition of the substantial organic matter and released into the atmosphere. In some fields, open burning is carried out by tradition, when carbon in residual matter is released into atmosphere as CO2. However, burning effect on carbon budget between crop lands and atmosphere is not entirely considered yet. In this study, coarse organic matter (COM) in paddy soil of a single-crop rice field was sampled on regular intervals between January, 2011 and August, 2014 The amount of carbon release from residual matter was estimated by analyzing of the variations in carbon content of COM. Effects of soil temperature (Ts) and soil water content (SWC) at the paddy field on the rate of carbon decomposition was investigated. Though decreasing rate of COM was much smaller in winter season, it is accelerated at the warming season between April and June every year. Decomposition was resisted for next rice cultivated season despite of highest soil temperature. In addition, the observational field was divided into two areas, and three time open burning experiments were conducted in November, 2011, 2012, and 2013. In each year, three sampling surveys, or plants before harvest and residuals before and after the burning experiment, were done. From these surveys, it is suggested that about 48±2% of carbon contents of above-ground plant was yield out as grain by harvest, and about 27±2% of carbon emitted as CO2 by burning. Carbon content of residuals plowed into soil after the harvest was estimated 293±1 and 220±36gC/m2 in no-burned and burned area, respectively, based on three-years average. It is estimated that 70 and 60% of the first input amount of COM was decomposed after a year in no-burned and burned area, respectively.

Spiritual and ceremonial plants in North America: an assessment of Moerman's ethnobotanical database comparing Residual, Binomial, Bayesian and Imprecise Dirichlet Model (IDM) analysis.

PubMed

Turi, Christina E; Murch, Susan J

2013-07-09

Ethnobotanical research and the study of plants used for rituals, ceremonies and to connect with the spirit world have led to the discovery of many novel psychoactive compounds such as nicotine, caffeine, and cocaine. In North America, spiritual and ceremonial uses of plants are well documented and can be accessed online via the University of Michigan's Native American Ethnobotany Database. The objective of the study was to compare Residual, Bayesian, Binomial and Imprecise Dirichlet Model (IDM) analyses of ritual, ceremonial and spiritual plants in Moerman's ethnobotanical database and to identify genera that may be good candidates for the discovery of novel psychoactive compounds. The database was queried with the following format "Family Name AND Ceremonial OR Spiritual" for 263 North American botanical families. Spiritual and ceremonial flora consisted of 86 families with 517 species belonging to 292 genera. Spiritual taxa were then grouped further into ceremonial medicines and items categories. Residual, Bayesian, Binomial and IDM analysis were performed to identify over and under-utilized families. The 4 statistical approaches were in good agreement when identifying under-utilized families but large families (>393 species) were underemphasized by Binomial, Bayesian and IDM approaches for over-utilization. Residual, Binomial, and IDM analysis identified similar families as over-utilized in the medium (92-392 species) and small (<92 species) classes. The families Apiaceae, Asteraceae, Ericacea, Pinaceae and Salicaceae were identified as significantly over-utilized as ceremonial medicines in medium and large sized families. Analysis of genera within the Apiaceae and Asteraceae suggest that the genus Ligusticum and Artemisia are good candidates for facilitating the discovery of novel psychoactive compounds. The 4 statistical approaches were not consistent in the selection of over-utilization of flora. Residual analysis revealed overall trends that were supported

The use of biogas plant fermentation residue for the stabilisation of toxic metals in agricultural soils

NASA Astrophysics Data System (ADS)

Geršl, Milan; Šotnar, Martin; Mareček, Jan; Vítěz, Tomáš; Koutný, Tomáš; Kleinová, Jana

2015-04-01

Our department has been paying attention to different methods of soil decontamination, including the in situ stabilisation. Possible reagents to control the toxic metals mobility in soils include a fermentation residue (FR) from a biogas plant. Referred to as digestate, it is a product of anaerobic decomposition taking place in such facilities. The fermentation residue is applied to soils as a fertiliser. A new way of its use is the in situ stabilisation of toxic metals in soils. Testing the stabilisation of toxic metals made use of real soil samples sourced from five agriculturally used areas of the Czech Republic with 3 soil samples taken from sites contaminated with Cu, Pb and Zn and 2 samples collected at sites of natural occurrence of Cu, Pb and Zn ores. All the samples were analysed using the sequential extraction procedure (BCR) (determine the type of Cu, Pb and Zn bonds). Stabilisation of toxic metals was tested in five soil samples by adding reagents as follows: dolomite, slaked lime, goethite, compost and fermentation residue. A single reagent was added at three different concentrations. In the wet state with the added reagents, the samples were left for seven days, shaken twice per day. After seven days, metal extraction was carried out: samples of 10 g soil were shaken for 2 h in a solution of 0.1M NH4NO3 at a 1:2.5 (g.ml-1), centrifuged for 15 min at 5,000 rpm and then filtered through PTFE 0.45 μm mesh filters. The extracts were analysed by ICP-OES. Copper The best reduction of Cu concentration in the extract was obtained at each of the tested sites by adding dolomite (10 g soil + 0.3 g dolomite). The concentration of Cu in the leachate decreased to 2.1-18.4% compare with the leachate without addition. Similar results were also shown for the addition of fermentation residue (10 g soil + 1 g FR). The Cu concentration in the leachate decreased to 16.7-26.8% compared with the leachate without addition. Lead The best results were achieved by adding

Susceptibility of materials processing experiments to low-level accelerations

NASA Technical Reports Server (NTRS)

Naumann, R. J.

1981-01-01

The types of material processing experiments being considered for shuttle can be grouped into four categories: (1) contained solidification experiment; (2) quasicontainerless experiments; (3) containerless experiments; and (4) fluids experiments. Low level steady acceleration, compensated and uncompensated transient accelerations, and rotation induced flow factors that must be considered in the acceleration environment of a space vehicle whose importance depends on the type of experiment being performed. Some control of these factors may be exercised by the location and orientation of the experiment relative to shuttle and by the orbit vehicle attitude chosen for mission. The effects of the various residual accelerations can have serious consequence to the control of the experiment and must be factored into the design and operation of the apparatus.

Progress toward Understanding Protein S-acylation: Prospective in Plants

PubMed Central

Li, Yaxiao; Qi, Baoxiu

2017-01-01

S-acylation, also known as S-palmitoylation or palmitoylation, is a reversible post-translational lipid modification in which long chain fatty acid, usually the 16-carbon palmitate, covalently attaches to a cysteine residue(s) throughout the protein via a thioester bond. It is involved in an array of important biological processes during growth and development, reproduction and stress responses in plant. S-acylation is a ubiquitous mechanism in eukaryotes catalyzed by a family of enzymes called Protein S-Acyl Transferases (PATs). Since the discovery of the first PAT in yeast in 2002 research in S-acylation has accelerated in the mammalian system and followed by in plant. However, it is still a difficult field to study due to the large number of PATs and even larger number of putative S-acylated substrate proteins they modify in each genome. This is coupled with drawbacks in the techniques used to study S-acylation, leading to the slower progress in this field compared to protein phosphorylation, for example. In this review we will summarize the discoveries made so far based on knowledge learnt from the characterization of protein S-acyltransferases and the S-acylated proteins, the interaction mechanisms between PAT and its specific substrate protein(s) in yeast and mammals. Research in protein S-acylation and PATs in plants will also be covered although this area is currently less well studied in yeast and mammalian systems. PMID:28392791

Accelerating plant breeding.

PubMed

De La Fuente, Gerald N; Frei, Ursula K; Lübberstedt, Thomas

2013-12-01

The growing demand for food with limited arable land available necessitates that the yield of major food crops continues to increase over time. Advances in marker technology, predictive statistics, and breeding methodology have allowed for continued increases in crop performance through genetic improvement. However, one major bottleneck is the generation time of plants, which is biologically limited and has not been improved since the introduction of doubled haploid technology. In this opinion article, we propose to implement in vitro nurseries, which could substantially shorten generation time through rapid cycles of meiosis and mitosis. This could prove a useful tool for speeding up future breeding programs with the aim of sustainable food production. Copyright © 2013 Elsevier Ltd. All rights reserved.

The Study of Residual Voltage of Induction Motor and the Influence of Various Parameters on the Residual Voltage

NASA Astrophysics Data System (ADS)

Zhang, Shuping; Zhao, Chen; Tan, Weipu

2017-05-01

The majority important load of industrial area is mainly composed of induction motor, it is more common that induction motor becomes sluggishness and even tripping due to the lose of power supply or other malfunction in the practical work. In this paper, space vector method is used to establish a reduced order model of induction motor, and then study the changes of motor electromagnetic after losing electricity. Based on motion equations of the rotor and magnetic flux conservation principle, it uses mathematical methods to deduce the expression of rotor current, rotor flux, the stator flux and the residual voltage of stator side. In addition, relying on thermal power plants, it uses the actual data of power plants, takes DIgsilent software to simulate the residual voltage of motor after losing electricity. analyses the influence on the residual voltage with the changes of the moment of inertia, load ratio, initial size of slip and the load characteristic of induction motor. By analysis of these, it has a more detailed understanding about the changes of residual voltage in practical application, in additional, it is more beneficial to put into standby power supply safely and effectively, moreover, reduce the influence of the input process to the whole system.

Utilization of corn residues for production of the polysaccharide schizophyllan

USDA-ARS?s Scientific Manuscript database

Abundant corn residues include fiber from wet milling operations and distillers' dried grains from dry grind ethanol plants. Biorefineries of the future will utilize such residues for the production of valuable bioproducts, particularly those traditionally produced from fossil fuels. Schizophyllan...

Systems analysis of a low-acceleration research facility

NASA Technical Reports Server (NTRS)

Martin, Gary L.; Ferebee, Melvin J., Jr.; Wright, Robert L.

1988-01-01

The Low-Acceleration Research Facility (LARF), an unmanned free-flier that is boosted from low-earth orbit to a desired altitude using an orbital transfer vehicle is discussed. Design techniques used to minimize acceleration-causing disturbances and to create an ultra-quiet workshop are discussed, focusing on residual acceleration induced by the environment, the spacecraft and experiments. The selection and integration of critical subsystems, such as electrical power and thermal control, that enable the LARf to accomodate sub-microgravity levels for extended periods of time are presented, including a discussion of the Low-Acceleration Module, which will supply the payload with 25.0 kW of power, and up to 11.8 kW in the low-power mode. Also, the data management, communications, guidance, navigation and control, and structural features of supporting subsystems are examined.

Thermal treatment of stabilized air pollution control residues in a waste incinerator pilot plant. Part 2: Leaching characteristics of bottom ashes.

PubMed

Baun, Dorthe L; Christensen, Thomas H; Bergfeldt, Brita; Vehlow, Jürgen; Mogensen, Erhardt P B

2004-02-01

With the perspective of generating only one solid residue from waste incineration, co-feeding of municipal solid waste and air pollution control residues stabilized by the Ferrox process was investigated in the TAMARA pilot plant incinerator as described in Bergfeldt et al. (Waste Management Research, 22, 49-57, 2004). This paper reports on leaching from the combined bottom ashes. Batch leaching test, pH-static leaching tests, availability tests and column leaching tests were used to characterize the leaching properties. The leaching properties are key information in the context of reuse in construction or in landfilling of the combined residue. In general, the combined bottom ashes had leaching characteristics similar to the reference bottom ash, which contained no APC residue. However, As and Pb showed slightly elevated leaching from the combined bottom ashes, while Cr showed less leaching. The investigated combined bottom ashes had contents of metals comparable to what is expected at steady state after continuous co-feeding of APC residues. Only Cd and Pb were partly volatilized (30-40%) during the incineration process and thus the combined bottom ashes had lower contents of Cd and Pb than expected at steady state. Furthermore, a major loss of Hg was, not surprisingly, seen and co-feeding of Ferrox-products together with municipal solid waste will require dedicated removal of Hg in the flue gas to prevent a build up of Hg in the system. In spite of this, a combined single solid residue from waste incineration seems to be a significant environmental improvement to current technology.

Transformation of corn plant residues in loamy and sandy substrates

NASA Astrophysics Data System (ADS)

Mal'tseva, A. N.; Zolotareva, B. N.; Pinskii, D. L.

2014-05-01

The mineralization and humification dynamics of corn plant residues in loamy and sandy substrates have been studied under laboratory conditions. It has been shown that the dynamics are determined by the undulating development laws of the microbial community under constant temperature and moisture conditions. At the same time, the intensity and final results of the processes significantly differ depending on the composition and properties of the mineral substrate. The loss of Corg during the mineralization and the content of newly formed humic substances reached the maximum values a month after the beginning of the experiment. The mineralization is more intensive in sand at the early stages, and the humification is more active in loam throughout the incubation period. The loamy substrate has better protective properties compared to the sand; therefore, it favors the accumulation of significant amounts of fulvic acids (FAs), along with humic acids (HAs), and causes the relative fulvatization of the humic substances. It has been found using densimetric fractionation and Fourier IR spectroscopy that the different mineralogy of the fractions results in differences in the chemical composition of the formed mineral-organic compounds of newly formed humic substances, mainly due to carboxyl and nitrogen-containing groups. The similarity of the humification products in the heavy fractions of the loamy and sandy substrates has been revealed.

Cancellation of residual spacecraft accelerations for zero-G space physics experiments

NASA Technical Reports Server (NTRS)

Stephens, J. B.

1977-01-01

The Drop Dynamics Module houses an acoustic positioning system which counteracts the effects of small accelerations of a spacecraft and thus allows long-term study of free-floating materials such as liquid drops. The acoustic positioning system provides an acoustic 'well' in the center of the experiment chamber. Data collection is by cinematographic photography. The module subsystems are discussed.

Assessing crop residue cover as scene moisture conditions change

USDA-ARS?s Scientific Manuscript database

Crop residue or plant litter is the portion of a crop left in the field after harvest. Crop residues on the soil surface provide a first line of defense against water and wind erosion and reduce the amounts of soil, nutrients, and pesticides that reach streams and rivers. Thus, quantification of cro...

Do ungulates accelerate or decelerate nitrogen cycling?

USGS Publications Warehouse

Singer, F.J.; Schoenecker, K.A.

2003-01-01

Nitrogen (N) is an essential nutrient for plants and animals, and N may be limiting in many western US grassland and shrubland ungulate winter ranges. Ungulates may influence N pools and they may alter N inputs and outputs (losses) to the ecosystem in a number of ways. In this paper we compare the ecosystem effects of ungulate herbivory in two western national parks, Rocky Mountain National Park (RMNP), Colorado, and Yellowstone National Park (YNP), Wyoming. We compare ungulate herbivory effects on N pools, N fluxes, N yields, and plant productivity in the context of the accelerating and decelerating nutrient cycling scenarios [Ecology 79 (1998) 165]. We concluded that the YNP grasslands fit the accelerating nutrient cycling scenario for ungulate herbivory: in response to grazing, grassland plant species abundance was largely unaltered, net annual aboveground primary productivity (NAPP) was stimulated (except during drought), consumption of key N-rich forages by ungulates was moderate and their abundance was sustained, soil N mineralization rates doubled, N pools increased, aboveground N yield increased, and N concentrations increased in most grassland plant species. Grazing in grasslands in RMNP resulted in no consistent detectable acceleration or deceleration of nutrient cycling. Grazing effects in short willow and aspen vegetation types in RMNP fit the decelerating nutrient cycling scenario of Ritchie et al. [Ecology 79 (1998) 165]. Key N-rich forages declined due to herbivory (willows, aspen, herbaceous vegetation). Aboveground production declined, soil N mineralization rates declined, N pools declined (NO3− pools were 30% that of ungrazed controls), and aboveground N yield declined. We believe that the higher ungulate densities and rates of plant consumption in RMNP, large declines in N-rich forage plants, and possibly a tendency of ungulates to move N from willow and aspen vegetation types to other types in RMNP, contributed to deceleration of nutrient

Phytoavailability and mechanism of bound PAH residues in filed contaminated soils.

PubMed

Gao, Yanzheng; Hu, Xiaojie; Zhou, Ziyuan; Zhang, Wei; Wang, Yize; Sun, Bingqing

2017-03-01

Understanding the phytoavailability of bound residues of polycyclic aromatic hydrocarbons (PAHs) in soils is essential to assessing their environmental fate and risks. This study investigated the release and plant uptake of bound PAH residues (reference to parent compounds) in field contaminated soils after the removal of extractable PAH fractions. Plant pot experiments were performed in a greenhouse using ryegrass (Lolium multiflorum Lam.) to examine the phytoavailablility of bound PAH residues, and microcosm incubation experiments with and without the addition of artificial root exudates (AREs) or oxalic acid were conducted to examine the effect of root exudates on the release of bound PAH residues. PAH accumulation in the ryegrass after a 50-day growth period indicated that bound PAH residues were significantly phytoavailable. The extractable fractions, including the desorbing and non-desorbing fractions, dominated the total PAH concentrations in vegetated soils after 50 days, indicating the transfer of bound PAH residues to the extractable fractions. This transfer was facilitated by root exudates. The addition of AREs and oxalic acid to test soils enhanced the release of bound PAH residues into their extractable fractions, resulting in enhanced phytoavailability of bound PAH residues in soils. This study provided important information regarding environmental fate and risks of bound PAH residues in soils. Copyright © 2016 Elsevier Ltd. All rights reserved.

The Spallation Neutron Source accelerator system design

NASA Astrophysics Data System (ADS)

Henderson, S.; Abraham, W.; Aleksandrov, A.; Allen, C.; Alonso, J.; Anderson, D.; Arenius, D.; Arthur, T.; Assadi, S.; Ayers, J.; Bach, P.; Badea, V.; Battle, R.; Beebe-Wang, J.; Bergmann, B.; Bernardin, J.; Bhatia, T.; Billen, J.; Birke, T.; Bjorklund, E.; Blaskiewicz, M.; Blind, B.; Blokland, W.; Bookwalter, V.; Borovina, D.; Bowling, S.; Bradley, J.; Brantley, C.; Brennan, J.; Brodowski, J.; Brown, S.; Brown, R.; Bruce, D.; Bultman, N.; Cameron, P.; Campisi, I.; Casagrande, F.; Catalan-Lasheras, N.; Champion, M.; Champion, M.; Chen, Z.; Cheng, D.; Cho, Y.; Christensen, K.; Chu, C.; Cleaves, J.; Connolly, R.; Cote, T.; Cousineau, S.; Crandall, K.; Creel, J.; Crofford, M.; Cull, P.; Cutler, R.; Dabney, R.; Dalesio, L.; Daly, E.; Damm, R.; Danilov, V.; Davino, D.; Davis, K.; Dawson, C.; Day, L.; Deibele, C.; Delayen, J.; DeLong, J.; Demello, A.; DeVan, W.; Digennaro, R.; Dixon, K.; Dodson, G.; Doleans, M.; Doolittle, L.; Doss, J.; Drury, M.; Elliot, T.; Ellis, S.; Error, J.; Fazekas, J.; Fedotov, A.; Feng, P.; Fischer, J.; Fox, W.; Fuja, R.; Funk, W.; Galambos, J.; Ganni, V.; Garnett, R.; Geng, X.; Gentzlinger, R.; Giannella, M.; Gibson, P.; Gillis, R.; Gioia, J.; Gordon, J.; Gough, R.; Greer, J.; Gregory, W.; Gribble, R.; Grice, W.; Gurd, D.; Gurd, P.; Guthrie, A.; Hahn, H.; Hardek, T.; Hardekopf, R.; Harrison, J.; Hatfield, D.; He, P.; Hechler, M.; Heistermann, F.; Helus, S.; Hiatt, T.; Hicks, S.; Hill, J.; Hill, J.; Hoff, L.; Hoff, M.; Hogan, J.; Holding, M.; Holik, P.; Holmes, J.; Holtkamp, N.; Hovater, C.; Howell, M.; Hseuh, H.; Huhn, A.; Hunter, T.; Ilg, T.; Jackson, J.; Jain, A.; Jason, A.; Jeon, D.; Johnson, G.; Jones, A.; Joseph, S.; Justice, A.; Kang, Y.; Kasemir, K.; Keller, R.; Kersevan, R.; Kerstiens, D.; Kesselman, M.; Kim, S.; Kneisel, P.; Kravchuk, L.; Kuneli, T.; Kurennoy, S.; Kustom, R.; Kwon, S.; Ladd, P.; Lambiase, R.; Lee, Y. Y.; Leitner, M.; Leung, K.-N.; Lewis, S.; Liaw, C.; Lionberger, C.; Lo, C. C.; Long, C.; Ludewig, H.; Ludvig, J.; Luft, P.; Lynch, M.; Ma, H.; MacGill, R.; Macha, K.; Madre, B.; Mahler, G.; Mahoney, K.; Maines, J.; Mammosser, J.; Mann, T.; Marneris, I.; Marroquin, P.; Martineau, R.; Matsumoto, K.; McCarthy, M.; McChesney, C.; McGahern, W.; McGehee, P.; Meng, W.; Merz, B.; Meyer, R.; Meyer, R.; Miller, B.; Mitchell, R.; Mize, J.; Monroy, M.; Munro, J.; Murdoch, G.; Musson, J.; Nath, S.; Nelson, R.; Nelson, R.; O`Hara, J.; Olsen, D.; Oren, W.; Oshatz, D.; Owens, T.; Pai, C.; Papaphilippou, I.; Patterson, N.; Patterson, J.; Pearson, C.; Pelaia, T.; Pieck, M.; Piller, C.; Plawski, T.; Plum, M.; Pogge, J.; Power, J.; Powers, T.; Preble, J.; Prokop, M.; Pruyn, J.; Purcell, D.; Rank, J.; Raparia, D.; Ratti, A.; Reass, W.; Reece, K.; Rees, D.; Regan, A.; Regis, M.; Reijonen, J.; Rej, D.; Richards, D.; Richied, D.; Rode, C.; Rodriguez, W.; Rodriguez, M.; Rohlev, A.; Rose, C.; Roseberry, T.; Rowton, L.; Roybal, W.; Rust, K.; Salazer, G.; Sandberg, J.; Saunders, J.; Schenkel, T.; Schneider, W.; Schrage, D.; Schubert, J.; Severino, F.; Shafer, R.; Shea, T.; Shishlo, A.; Shoaee, H.; Sibley, C.; Sims, J.; Smee, S.; Smith, J.; Smith, K.; Spitz, R.; Staples, J.; Stein, P.; Stettler, M.; Stirbet, M.; Stockli, M.; Stone, W.; Stout, D.; Stovall, J.; Strelo, W.; Strong, H.; Sundelin, R.; Syversrud, D.; Szajbler, M.; Takeda, H.; Tallerico, P.; Tang, J.; Tanke, E.; Tepikian, S.; Thomae, R.; Thompson, D.; Thomson, D.; Thuot, M.; Treml, C.; Tsoupas, N.; Tuozzolo, J.; Tuzel, W.; Vassioutchenko, A.; Virostek, S.; Wallig, J.; Wanderer, P.; Wang, Y.; Wang, J. G.; Wangler, T.; Warren, D.; Wei, J.; Weiss, D.; Welton, R.; Weng, J.; Weng, W.-T.; Wezensky, M.; White, M.; Whitlatch, T.; Williams, D.; Williams, E.; Wilson, K.; Wiseman, M.; Wood, R.; Wright, P.; Wu, A.; Ybarrolaza, N.; Young, K.; Young, L.; Yourd, R.; Zachoszcz, A.; Zaltsman, A.; Zhang, S.; Zhang, W.; Zhang, Y.; Zhukov, A.

2014-11-01

The Spallation Neutron Source (SNS) was designed and constructed by a collaboration of six U.S. Department of Energy national laboratories. The SNS accelerator system consists of a 1 GeV linear accelerator and an accumulator ring providing 1.4 MW of proton beam power in microsecond-long beam pulses to a liquid mercury target for neutron production. The accelerator complex consists of a front-end negative hydrogen-ion injector system, an 87 MeV drift tube linear accelerator, a 186 MeV side-coupled linear accelerator, a 1 GeV superconducting linear accelerator, a 248-m circumference accumulator ring and associated beam transport lines. The accelerator complex is supported by ~100 high-power RF power systems, a 2 K cryogenic plant, ~400 DC and pulsed power supply systems, ~400 beam diagnostic devices and a distributed control system handling ~100,000 I/O signals. The beam dynamics design of the SNS accelerator is presented, as is the engineering design of the major accelerator subsystems.

Optimal Thermolysis Conditions for Soil Carbon Storage on Plant Residue Burning: Modeling the Trade-Off between Thermal Decomposition and Subsequent Biodegradation.

PubMed

Kajiura, Masako; Wagai, Rota; Hayashi, Kentaro

2015-01-01

Field burning of plant biomass is a widespread practice that provides charred materials to soils. Its impact on soil C sequestration remains unclear due to the heterogeneity of burning products and difficulty in monitoring the material's biodegradation in fields. Basic information is needed on the relationship between burning conditions and the resulting quantity/quality of residue-derived C altered by thermal decomposition and biodegradation. In this study, we thermolyzed residues (rice straw and husk) at different temperatures (200-600°C) under two oxygen availability conditions and measured thermal mass loss, C compositional change by solid-state C NMR spectroscopy, and biodegradability of the thermally altered residues by laboratory aerobic incubation. A trade-off existed between thermal and microbial decomposition: when burned at higher temperatures, residues experience a greater mass loss but become more recalcitrant via carbonization. When an empirical model accounting for the observed trade-off was projected over 10 to 10 yr, we identified the threshold temperature range (330-400°C) above and below which remaining residue C is strongly reduced. This temperature range corresponded to the major loss of O-alkyl C and increase in aromatic C. The O/C molar ratios of the resultant residues decreased to 0.2 to 0.4, comparable to those of chars in fire-prone field soils reported previously. Although the negative impacts of biomass burning need to be accounted for, the observed relationship may help to assess the long-term fate of burning-derived C and to enhance soil C sequestration. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

40 CFR 180.202 - p-Chlorophenoxyacetic acid; tolerances for residues.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 24 2014-07-01 2014-07-01 false p-Chlorophenoxyacetic acid; tolerances... Tolerances § 180.202 p-Chlorophenoxyacetic acid; tolerances for residues. (a) General. A tolerance is established for the combined residues of the plant regulator p-chlorophenoxyacetic acid and its metabolite p...

40 CFR 180.202 - p-Chlorophenoxyacetic acid; tolerances for residues.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 25 2013-07-01 2013-07-01 false p-Chlorophenoxyacetic acid; tolerances... Tolerances § 180.202 p-Chlorophenoxyacetic acid; tolerances for residues. (a) General. A tolerance is established for the combined residues of the plant regulator p-chlorophenoxyacetic acid and its metabolite p...

40 CFR 180.202 - p-Chlorophenoxyacetic acid; tolerances for residues.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 25 2012-07-01 2012-07-01 false p-Chlorophenoxyacetic acid; tolerances... Tolerances § 180.202 p-Chlorophenoxyacetic acid; tolerances for residues. (a) General. A tolerance is established for the combined residues of the plant regulator p-chlorophenoxyacetic acid and its metabolite p...

Influence of biochar and plant growth on organic matter dynamics in a reclaimed mine residue

NASA Astrophysics Data System (ADS)

Moreno-Barriga, Fabián; Díaz, Vicente; Alberto, Jose; Faz, Ángel; Zornoza, Raúl

2016-04-01

This study aims at assessing the impact of biochar and marble waste amendment and the development of vegetation in acidic mine wastes on organic matter dynamics. For this purpose, a mine residue was collected in a tailing pond from the Sierra Minera of Cartagena-La Unión (SE Spain), and a greenhouse experiment was established for 120 days. Marble waste (MW) was added in a rate of 200 g kg-1 as a source of calcium carbonate to increase the pH from 3 to 7.5-8 (average pH in the native soils of the area). We added biochar as a source of organic carbon and nutrients, in two different rates, 50 g kg-1 (BC1) and 100 g kg-1 (BC2). To assess the influence of vegetation growth on the creation of a technosoil from mine residues and its impact on organic matter dynamics, the plant species Piptatherum miliaceum (PM) was planted in half the pots with the different amendments. Thus, five treatments were established: unamended and unplanted control (CT), BC1, BC2, BC1+PM and BC2+PM. Results showed that the different treatments had no significant effect on aggregates stability, microbial biomass carbon and the emission of N2O and CH4. So, it seems that longer periods are needed to increase the stability of aggregates and microbial populations, since even the combined use of biochar, marble waste and vegetation was not enough to increase these properties in 120 days. Nonetheless, it was positive that the addition of biochar and the release of root exudates did not trigger the emission of greenhouse gases. Organic carbon significantly increased with the addition of biochar, with values similar to the dose applied, indicating high stability and low mineralization of the amendment. The addition of amendments significantly increased arylesterase activity, while the growth of the plant was needed to significantly increase β-glucosidase activity. The soluble carbon significantly decreased in BC1 and BC2 with regards to CT, while no significant differences were observed among CT and

Arsenic stabilization by zero-valent iron, bauxite residue, and zeolite at a contaminated site planting Panax notoginseng.

PubMed

Yan, X L; Lin, L Y; Liao, X Y; Zhang, W B; Wen, Y

2013-10-01

Panax notoginseng (Burk.) F.H. Chen, a rare traditional Chinese medicinal herb, is a widely used phytomedicine used all over the world. In recent years, the arsenic contamination of the herb and its relative products becomes a serious problem due to elevated soil As concentration. This study aimed to evaluate the effects of different types and dosages of amendments on As stabilization in soil and its uptake by P. notoginseng. Results showed that comparing to control treatment, the As concentrations of P. notoginseng declined by 49-63%, 43-61% and 52-66% in 0.25% zero-valent iron (Fe(0)), 0.5% bauxite residue, and 1% zeolite treatment, respectively; whereas the biomasses were elevated by 62-116%, 45-152% and 114-265%, respectively. The As(III) proportions of P. notoginseng increased by 8%, 9%, and 8%, and the transfer factors of As from root to shoot increased by 37%, 42% and 84% in the optimal treatments of Fe(0), bauxite residue, and zeolite. For soil As, all the three amendments could transform the non-specifically adsorbed As fraction to hydrous oxides Fe/Al fractions (by Fe(0) and red mud) or specifically adsorbed As fraction (by zeolite), therefore reduced the bioavailability of soil As. With a comprehensive consideration of stabilization efficiency, plant growth, environmental influence, and cost, Fe(0) appeared to be the best amendment, and zeolite could also be a good choice. In conclusion, this study was of significance in developing As contamination control in P. notoginseng planting areas, and even other areas for medicinal herb growing. Copyright © 2013 Elsevier Ltd. All rights reserved.

Recovery of forest residues in the Southern United States

Treesearch

Bryce J. Stokes; Donald L. Sirois

1989-01-01

In the mid 1970's, the accelerated price increases for petroleum products forced rapid exploration into and adoption of alternative energy sources. A viable option for the forest industry was the recovery of woody biomass from unmerchantable trees and logging residues. Several studies estimated that an abundance of such forest materials existed in the southeastern...

Crop residue decomposition in Minnesota biochar-amended plots

NASA Astrophysics Data System (ADS)

Weyers, S. L.; Spokas, K. A.

2014-06-01

Impacts of biochar application at laboratory scales are routinely studied, but impacts of biochar application on decomposition of crop residues at field scales have not been widely addressed. The priming or hindrance of crop residue decomposition could have a cascading impact on soil processes, particularly those influencing nutrient availability. Our objectives were to evaluate biochar effects on field decomposition of crop residue, using plots that were amended with biochars made from different plant-based feedstocks and pyrolysis platforms in the fall of 2008. Litterbags containing wheat straw material were buried in July of 2011 below the soil surface in a continuous-corn cropped field in plots that had received one of seven different biochar amendments or a uncharred wood-pellet amendment 2.5 yr prior to start of this study. Litterbags were collected over the course of 14 weeks. Microbial biomass was assessed in treatment plots the previous fall. Though first-order decomposition rate constants were positively correlated to microbial biomass, neither parameter was statistically affected by biochar or wood-pellet treatments. The findings indicated only a residual of potentially positive and negative initial impacts of biochars on residue decomposition, which fit in line with established feedstock and pyrolysis influences. Overall, these findings indicate that no significant alteration in the microbial dynamics of the soil decomposer communities occurred as a consequence of the application of plant-based biochars evaluated here.

Effects of plant polyphenols and a-tocopherol on lipid oxidation, residual nitrites, biogenic amines, and N-nitrosamines formation during ripening and storage of dry-cured bacon

USDA-ARS?s Scientific Manuscript database

Effects of plant polyphenols (green tea polyphenols (GTP) and grape seed extract (GSE) and a-tocopherol on physicochemical parameters, lipid oxidation, residual nitrite, microbiological counts, biogenic amines, and N-nitrosamines were determined in bacons during dry-curing and storage. Results show ...

Comparative evaluation of thermal oxidative decomposition for oil-plant residues via thermogravimetric analysis: Thermal conversion characteristics, kinetics, and thermodynamics.

PubMed

Chen, Jianbiao; Wang, Yanhong; Lang, Xuemei; Ren, Xiu'e; Fan, Shuanshi

2017-11-01

Thermal oxidative decomposition characteristics, kinetics, and thermodynamics of rape straw (RS), rapeseed meal (RM), camellia seed shell (CS), and camellia seed meal (CM) were evaluated via thermogravimetric analysis (TGA). TG-DTG-DSC curves demonstrated that the combustion of oil-plant residues proceeded in three stages, including dehydration, release and combustion of organic volatiles, and chars oxidation. As revealed by combustion characteristic parameters, the ignition, burnout, and comprehensive combustion performance of residues were quite distinct from each other, and were improved by increasing heating rate. The kinetic parameters were determined by Coats-Redfern approach. The results showed that the most possible combustion mechanisms were order reaction models. The existence of kinetic compensation effect was clearly observed. The thermodynamic parameters (ΔH, ΔG, ΔS) at peak temperatures were calculated through the activated complex theory. With the combustion proceeding, the variation trends of ΔH, ΔG, and ΔS for RS (RM) similar to those for CS (CM). Copyright © 2017 Elsevier Ltd. All rights reserved.

REGULATIONS ON THE DISPOSAL OF ARSENIC RESIDUALS FROM DRINKING WATER TREATMENT PLANTS

EPA Science Inventory

This report summarizes federal and selected state regulations that govern the management of residuals produced by small water treatment systems removing arsenic from drinking water. The document focuses on the residuals produced by five treatment processes: anion exchange, activa...

The effectiveness of arbuscular-mycorrhizal fungi and Aspergillus niger or Phanerochaete chrysosporium treated organic amendments from olive residues upon plant growth in a semi-arid degraded soil.

PubMed

Medina, A; Roldán, A; Azcón, R

2010-12-01

Arbuscular mycorrhizal (AM) fungi and a residue from dry olive cake (DOC) supplemented with rock phosphate (RP) and treated with either Aspergillus niger (DOC-A) or Phanerochaete chrysosporium (DOC-P), were assayed in a natural, semi-arid soil using Trifolium repens or Dorycnium pentaphyllum plants. The effects of the AM fungi and/or DOC-A were compared with P-fertilisation (P) over eleven successive harvests to evaluate the persistence of the effectiveness of the treatments. The biomass of dually-treated plants after four successive harvests was greater than that obtained for non-treated plants or those receiving the AM inoculum or DOC-A treatments after eleven yields. The AM inoculation was critical for obtaining plant growth benefit from the application of fermented DOC-A residue. The abilities of the treatments to prevent plant drought stress were also assayed. Drought-alleviating effects were evaluated in terms of plant growth, proline and total sugars concentration under alternative drought and re-watering conditions (8th and 9th harvests). The concentrations of both compounds in plant biomass increased under drought when DOC-A amendment and AM inoculation were employed together: they reinforced the plant drought-avoidance capabilities and anti-oxidative defence. Water stress was less compensated in P-fertilised than in DOC-A-treated plants. DOC-P increased D. pentaphyllum biomass, shoot P content, nodule number and AM colonisation, indicating the greater DOC-transforming ability of P. chrysosporium compared to A. niger. The lack of AM colonisation and nodulation in this soil was compensated by the application of DOC-P, particularly with AM inoculum. The management of natural resources (organic amendments and soil microorganisms) represents an important strategy that assured the growth, nutrition and plant establishment in arid, degraded soils, preventing the damage that arises from limited water and nutrient supply. Copyright © 2010 Elsevier Ltd. All rights

Direct observation of the residual plastic deformation caused by a single tensile overload

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bichler, C.; Pippan, R.

1999-07-01

The fatigue crack growth behavior following single tensile overloads at high stress intensity ranges in a cold-rolled austenitic steel has been studied experimentally. After tensile overloads, fatigue cracks initially accelerate, followed by significant retardation, before the growth rates return to their baseline level. The initial acceleration was attributed to an immediate reduction in near-tip closure. Scanning electron micrography and stereophotogrammetric reconstruction of the fracture surface were applied to study the residual plastic deformation caused by a single tensile overload in the mid-thickness of the specimen. The measured residual opening displacement of the crack as a function of the overload ismore » presented and compared with simple estimations. Also, free specimen surface observations of the residual plastic deformation and crack growth rate were performed. In the midsection of the specimens the striation spacing-length, i.e., the microscopic growth rates, were measured before and after the applied overload. It will be shown that the measured plasticity-induced wedges from the single overload and the observed propagation behavior support the significance of the concept of crack closure.« less

Flow Accelerated Erosion-Corrosion (FAC) considerations for secondary side piping in the AP1000{sup R} nuclear power plant design

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vanderhoff, J. F.; Rao, G. V.; Stein, A.

2012-07-01

The issue of Flow Accelerated Erosion-Corrosion (FAC) in power plant piping is a known phenomenon that has resulted in material replacements and plant accidents in operating power plants. Therefore, it is important for FAC resistance to be considered in the design of new nuclear power plants. This paper describes the design considerations related to FAC that were used to develop a safe and robust AP1000{sup R} plant secondary side piping design. The primary FAC influencing factors include: - Fluid Temperature - Pipe Geometry/layout - Fluid Chemistry - Fluid Velocity - Pipe Material Composition - Moisture Content (in steam lines) Duemore » to the unknowns related to the relative impact of the influencing factors and the complexities of the interactions between these factors, it is difficult to accurately predict the expected wear rate in a given piping segment in a new plant. This paper provides: - a description of FAC and the factors that influence the FAC degradation rate, - an assessment of the level of FAC resistance of AP1000{sup R} secondary side system piping, - an explanation of options to increase FAC resistance and associated benefits/cost, - discussion of development of a tool for predicting FAC degradation rate in new nuclear power plants. (authors)« less

Effects of Experiment Location and Orbiter Attitude on the Residual Acceleration On-Board STS-73 (USML-2)

NASA Technical Reports Server (NTRS)

Hakimzadeh, Roshanak; McPherson, Kevin M.; Matisak, Brian P.; Wagar, William O.

1997-01-01

A knowledge of the quasi-steady acceleration environment on the NASA Space Shuttle Orbiter is of particular importance for materials processing experiments which are limited by slow diffusive processes. The quasi-steady (less than 1 HZ) acceleration environment on STS-73 (USML-2) was measured using the Orbital Acceleration Research Experiment (OARE) accelerometer. One of the facilities flown on USML-2 was the Crystal Growth Furnace (CGF), which was used by several Principal Investigators (PIS) to grow crystals. In this paper the OARE data mapped to the sample melt location within this furnace is presented. The ratio of the axial to radial components of the quasi-steady acceleration at the melt site is presented. Effects of Orbiter attitude on the acceleration data is discussed.

Tillage as a tool to manage crop residue: impact on sugar beet production.

NASA Astrophysics Data System (ADS)

Hiel, Marie-Pierre; Chélin, Marie; Degrune, Florine; Parvin, Nargish; Bodson, Bernard

2015-04-01

Crop residues and plant cover represent a pool of organic matter that can be used either to restore organic matter in soils, and therefore maintain soil fertility, or that can be valorized outside of the field (e.g. energy production). However, it is crucial that the exportation of residues is not done to the detriment of the system sustainability. Three long term experiments have been settled in the loamy region in Belgium. All of them are designed to study the effect of residues management by several tillage systems (conventional plowing versus reduced tillage) on the whole soil-water-plant system. SOLRESIDUS is a field experiment where we study the impact of crop residue management while in SOLCOUVERT and SOLCOUVERT-BIS, we study the impact of cover crop management. SOLRESIDUS was started in 2008. In this field, four contrasted crop residues managements are tested in order to contrast as much as possible the responses from the soil-water plant system. Two practices characterize the four modalities: soil tillage (ploughing at 25 cm depth or reduce tillage at 10 cm max) and residue management (exportation or restitution). SOLCOUVERT and SOLCOUVERT-BIS were started in 2012 and 2013 respectively. In those fields cover crop management is also diverse: destruction of the cover crop by winter ploughing, spring ploughing, strip tillage (with a chemical destruction if needed) or shallow tillage (with a decompaction before cover crop sowing). Although although the overall project aims at studying the impact of management on the whole soil-water-plant system, here we will only present the results concerning crop production (sugar beet) in SOLCOUVERT experiments. The presented data will include germination rate, crop development (biomass quantification and BBCH stages) weeds population, disease occurrence, pest occurrences, nitrogen uptake by plants, quality and quantity of harvested products.

Efficient stabilization and acceleration of numerical simulation of fluid flows by residual recombination

NASA Astrophysics Data System (ADS)

Citro, V.; Luchini, P.; Giannetti, F.; Auteri, F.

2017-09-01

The study of the stability of a dynamical system described by a set of partial differential equations (PDEs) requires the computation of unstable states as the control parameter exceeds its critical threshold. Unfortunately, the discretization of the governing equations, especially for fluid dynamic applications, often leads to very large discrete systems. As a consequence, matrix based methods, like for example the Newton-Raphson algorithm coupled with a direct inversion of the Jacobian matrix, lead to computational costs too large in terms of both memory and execution time. We present a novel iterative algorithm, inspired by Krylov-subspace methods, which is able to compute unstable steady states and/or accelerate the convergence to stable configurations. Our new algorithm is based on the minimization of the residual norm at each iteration step with a projection basis updated at each iteration rather than at periodic restarts like in the classical GMRES method. The algorithm is able to stabilize any dynamical system without increasing the computational time of the original numerical procedure used to solve the governing equations. Moreover, it can be easily inserted into a pre-existing relaxation (integration) procedure with a call to a single black-box subroutine. The procedure is discussed for problems of different sizes, ranging from a small two-dimensional system to a large three-dimensional problem involving the Navier-Stokes equations. We show that the proposed algorithm is able to improve the convergence of existing iterative schemes. In particular, the procedure is applied to the subcritical flow inside a lid-driven cavity. We also discuss the application of Boostconv to compute the unstable steady flow past a fixed circular cylinder (2D) and boundary-layer flow over a hemispherical roughness element (3D) for supercritical values of the Reynolds number. We show that Boostconv can be used effectively with any spatial discretization, be it a finite

Antimicrobial Peptides from Plants

PubMed Central

Tam, James P.; Wang, Shujing; Wong, Ka H.; Tan, Wei Liang

2015-01-01

Plant antimicrobial peptides (AMPs) have evolved differently from AMPs from other life forms. They are generally rich in cysteine residues which form multiple disulfides. In turn, the disulfides cross-braced plant AMPs as cystine-rich peptides to confer them with extraordinary high chemical, thermal and proteolytic stability. The cystine-rich or commonly known as cysteine-rich peptides (CRPs) of plant AMPs are classified into families based on their sequence similarity, cysteine motifs that determine their distinctive disulfide bond patterns and tertiary structure fold. Cystine-rich plant AMP families include thionins, defensins, hevein-like peptides, knottin-type peptides (linear and cyclic), lipid transfer proteins, α-hairpinin and snakins family. In addition, there are AMPs which are rich in other amino acids. The ability of plant AMPs to organize into specific families with conserved structural folds that enable sequence variation of non-Cys residues encased in the same scaffold within a particular family to play multiple functions. Furthermore, the ability of plant AMPs to tolerate hypervariable sequences using a conserved scaffold provides diversity to recognize different targets by varying the sequence of the non-cysteine residues. These properties bode well for developing plant AMPs as potential therapeutics and for protection of crops through transgenic methods. This review provides an overview of the major families of plant AMPs, including their structures, functions, and putative mechanisms. PMID:26580629

Characteristics of purple nonsulfur bacteria grown under Stevia residue extractions.

PubMed

Xu, J; Feng, Y; Wang, Y; Lin, X

2013-11-01

As a consequence of the large-scale cultivation of Stevia plants, releases of plant residues, the byproduct after sweetener extraction, to the environment are inevitable. Stevia residue and its effluent after batching up contain large amounts of organic matters with small molecular weight, which therefore are a potential pollution source. Meanwhile, they are favourite substrates for micro-organism growths. This investigation was aimed to utilize the simulated effluent of Stevia residue to enrich the representative purple nonsulfur bacterium (PNSB), Rhodopseudomonas palustris (Rps. palustris), which has important economic values. The growth profile and quality of Rps. palustris were characterized by spectrophotometry, compared to those grown in common PNSB mineral synthetic medium. Our results revealed that the simulated effluent of Stevia residue not only stimulated Rps. palustris growth to a greater extent, but also increased its physiologically active cytochrome concentrations and excreted indole-3-acetic acid (IAA) content. This variation in phenotype of Rps. palustris could result from the shift in its genotype, further revealed by the repetitive sequence-based PCR (rep-PCR) fingerprinting analysis. Our results showed that the effluent of Stevia residue was a promising substrate for microbial growth. © 2013 The Society for Applied Microbiology.

Minimizing residual aluminum concentration in treated water by tailoring properties of polyaluminum coagulants.

PubMed

Kimura, Masaoki; Matsui, Yoshihiko; Kondo, Kenta; Ishikawa, Tairyo B; Matsushita, Taku; Shirasaki, Nobutaka

2013-04-15

Aluminum coagulants are widely used in water treatment plants to remove turbidity and dissolved substances. However, because high aluminum concentrations in treated water are associated with increased turbidity and because aluminum exerts undeniable human health effects, its concentration should be controlled in water treatment plants, especially in plants that use aluminum coagulants. In this study, the effect of polyaluminum chloride (PACl) coagulant characteristics on dissolved residual aluminum concentrations after coagulation and filtration was investigated. The dissolved residual aluminum concentrations at a given coagulation pH differed among the PACls tested. Very-high-basicity PACl yielded low dissolved residual aluminum concentrations and higher natural organic matter (NOM) removal. The low residual aluminum concentrations were related to the low content of monomeric aluminum (Ala) in the PACl. Polymeric (Alb)/colloidal (Alc) ratio in PACl did not greatly influence residual aluminum concentration. The presence of sulfate in PACl contributed to lower residual aluminum concentration only when coagulation was performed at around pH 6.5 or lower. At a wide pH range (6.5-8.5), residual aluminum concentrations <0.02 mg/L were attained by tailoring PACl properties (Ala percentage ≤0.5%, basicity ≥85%). The dissolved residual aluminum concentrations did not increase with increasing the dosage of high-basicity PACl, but did increase with increasing the dosage of normal-basicity PACl. We inferred that increasing the basicity of PACl afforded lower dissolved residual aluminum concentrations partly because the high-basicity PACls could have a small percentage of Ala, which tends to form soluble aluminum-NOM complexes with molecular weights of 100 kDa-0.45 μm. Copyright © 2013 Elsevier Ltd. All rights reserved.

Zinc Extraction from Zinc Plants Residue Using Selective Alkaline Leaching and Electrowinning

NASA Astrophysics Data System (ADS)

Ashtari, Pedram; Pourghahramani, Parviz

2015-10-01

Annually, a great amount of zinc plants residue is produced in Iran. One of them is hot filter cake (known as HFC) which can be used as a secondary resource of zinc, cobalt and manganese. Unfortunately, despite its heavy metal content, the HFC is not treated. For the first time, zinc was selectively leached from HFC employing alkaline leaching. Secondly, leaching was optimized to achieve maximum recovery using this method. Effects of factors like NaOH concentration (C = 3, 5, 7 and 9 M), temperature (T = 50, 70, 90 and 105 °C), solid/liquid ratio (weight/volume, S/L = 1/10 and 1/5 W/V) and stirring speed (R = 500 and 800 rpm) were studied on HFC leaching. L16 orthogonal array (OA, two factors in four levels and two factors in two levels) was applied to determine the optimum condition and the most significant factor affecting the overall zinc extraction. As a result, maximum zinc extraction was 83.4 %. Afterwards, a rough test was conducted for zinc electrowinning from alkaline solution according to the common condition available in literature by which pure zinc powder (99.96 %) was successfully obtained.

Accelerator-driven Transmutation of Waste

NASA Astrophysics Data System (ADS)

Venneri, Francesco

1998-04-01

Nuclear waste from commercial power plants contains large quantities of plutonium, other fissionable actinides, and long-lived fission products that are potential proliferation concerns and create challenges for the long-term storage. Different strategies for dealing with nuclear waste are being followed by various countries because of their geologic situations and their views on nuclear energy, reprocessing and non-proliferation. The current United States policy is to store unprocessed spent reactor fuel in a geologic repository. Other countries are opting for treatment of nuclear waste, including partial utilization of the fissile material contained in the spent fuel, prior to geologic storage. Long-term uncertainties are hampering the acceptability and eventual licensing of a geologic repository for nuclear spent fuel in the US, and driving up its cost. The greatest concerns are with the potential for radiation release and exposure from the spent fuel for tens of thousands of years and the possible diversion and use of the actinides contained in the waste for weapons construction. Taking advantage of the recent breakthroughs in accelerator technology and of the natural flexibility of subcritical systems, the Accelerator-driven Transmutation of Waste (ATW) concept offers the United States and other countries the possibility to greatly reduce plutonium, higher actinides and environmentally hazardous fission products from the waste stream destined for permanent storage. ATW does not eliminate the need for, but instead enhances the viability of permanent waste repositories. Far from being limited to waste destruction, the ATW concept also brings to the table new technologies that could be relevant for next-generation power producing reactors. In the ATW concept, spent fuel would be shipped to the ATW site where the plutonium, transuranics and selected long-lived fission products would be destroyed by fission or transmutation in their first and only pass through the

TECHNOLOGY TRANSFER HANDBOOK: MANAGEMENT OF WATER TREATMENT PLANT RESIDUALS

EPA Science Inventory

Potable water treatment processes produce safe drinking water and generate a wide variety of waste products known as residuals, including organic and inorganic compounds in liquid, solid, and gaseous forms. In the current regulatory climate, a complete management program for a w...

Enterococcus faecium LKE12 Cell-Free Extract Accelerates Host Plant Growth via Gibberellin and Indole-3-Acetic Acid Secretion.

PubMed

Lee, Ko-Eun; Radhakrishnan, Ramalingam; Kang, Sang-Mo; You, Young-Hyun; Joo, Gil-Jae; Lee, In-Jung; Ko, Jae-Hwan; Kim, Jin-Ho

2015-09-01

The use of microbial extracts containing plant hormones is a promising technique to improve crop growth. Little is known about the effect of bacterial cell-free extracts on plant growth promotion. This study, based on phytohormonal analyses, aimed at exploring the potential mechanisms by which Enterococcus faecium LKE12 enhances plant growth in oriental melon. A bacterial strain, LKE12, was isolated from soil, and further identified as E. faecium by 16S rDNA sequencing and phylogenetic analysis. The plant growth-promoting ability of an LKE12 bacterial culture was tested in a gibberellin (GA)-deficient rice dwarf mutant (waito-C) and a normal GA biosynthesis rice cultivar (Hwayongbyeo). E. faecium LKE12 significantly improved the length and biomass of rice shoots in both normal and dwarf cultivars through the secretion of an array of gibberellins (GA1, GA3, GA7, GA8, GA9, GA12, GA19, GA20, GA24, and GA53), as well as indole-3-acetic acid (IAA). To the best of our knowledge, this is the first study indicating that E. faecium can produce GAs. Increases in shoot and root lengths, plant fresh weight, and chlorophyll content promoted by E. faecium LKE12 and its cell-free extract inoculated in oriental melon plants revealed a favorable interaction of E. faecium LKE12 with plants. Higher plant growth rates and nutrient contents of magnesium, calcium, sodium, iron, manganese, silicon, zinc, and nitrogen were found in cell-free extract-treated plants than in control plants. The results of the current study suggest that E. faecium LKE12 promotes plant growth by producing GAs and IAA; interestingly, the exogenous application of its cell-free culture extract can be a potential strategy to accelerate plant growth.

Hyperspectral imaging technique for detection of poultry fecal residues on food processing equipments

NASA Astrophysics Data System (ADS)

Cho, Byoung-Kwan; Kim, Moon S.; Chen, Yud-Ren

2005-11-01

Emerging concerns about safety and security in current mass production of food products necessitate rapid and reliable inspection for contaminant-free products. Diluted fecal residues on poultry processing plant equipment surface, not easily discernable from water by human eye, are contamination sources for poultry carcasses. Development of sensitive detection methods for fecal residues is essential to ensure safe production of poultry carcasses. Hyperspectral imaging techniques have shown good potential for detecting of the presence of fecal and other biological substances on food and processing equipment surfaces. In this study, use of high spatial resolution hyperspectral reflectance and fluorescence imaging (with UV-A excitation) is presented as a tool for selecting a few multispectral bands to detect diluted fecal and ingesta residues on materials used for manufacturing processing equipment. Reflectance and fluorescence imaging methods were compared for potential detection of a range of diluted fecal residues on the surfaces of processing plant equipment. Results showed that low concentrations of poultry feces and ingesta, diluted up to 1:100 by weight with double distilled water, could be detected using hyperspectral fluorescence images with an accuracy of 97.2%. Spectral bands determined in this study could be used for developing a real-time multispectral inspection device for detection of harmful organic residues on processing plant equipment.

Accelerating global access to plant diversity information.

PubMed

Lughadha, Eimear Nic; Miller, Chuck

2009-11-01

Botanic gardens play key roles in the development and dissemination of plant information resources. Drivers for change have included progress in information technology, growing public expectations of electronic access and international conservation policy. Great advances have been made in the quantity, quality and accessibility of plant information in digital form and the extent to which information from multiple providers can be accessed through a single portal. However, significant challenges remain to be addressed in making botanic gardens resources maximally accessible and impactful, not least the overwhelming volume of material which still awaits digitisation. The year 2010 represents an opportunity for botanic gardens to showcase their collaborative achievements in delivery of electronic plant information and reinforce their relevance to pressing environmental issues.

Major pharmaceutical residues in wastewater treatment plants and receiving waters in Bangkok, Thailand, and associated ecological risks.

PubMed

Tewari, S; Jindal, R; Kho, Y L; Eo, S; Choi, K

2013-04-01

Pharmaceuticals have been frequently detected in aquatic environment worldwide and suspected for potential ecological consequences. However, occurrences, sources and potential risks of pharmaceutical residues have rarely been investigated in Bangkok, Thailand, one of most densely populated cities in the world. We collected water samples from five wastewater treatment plants (WWTPs), six canals, and in mainstream Chao Phraya River of Bangkok, in three sampling events representing different seasonal flow conditions, i.e., June and September 2011 and January 2012. Fourteen major pharmaceuticals including acetaminophen, acetylsalicylic acid, atenolol, caffeine, ciprofloxacin, diclofenac, ibuprofen, mefenamic acid, naproxen, roxithromycin, sulfamethazine, sulfamethoxazole, sulfathiazole and trimethoprim were analyzed. Levels of pharmaceutical residues in WWTP influents on average were the highest for acetylsalicylic acid (4700 ng L(-1)), followed by caffeine (2250 ng L(-1)) and ibuprofen (702 ng L(-1)). In effluents, the concentration of caffeine was the highest (307 ng L(-1)), followed by acetylsalicylic acid (261 ng L(-1)) and mefenamic acid (251 ng L(-1)). In surface water, acetylsalicylic acid showed the highest levels (on average 1360 ng L(-1) in canals and 313 ng L(-1) in the river). Removal efficiencies of WWTPs for roxithromycin, sulfamethoxazole and sulfamethazine were determined negligible. For several compounds, the concentrations in ambient water were higher than those detected in the effluents, implying contribution of the WWTPs to be negligible. Hazard quotients estimated for acetylsalicylic acid, ciprofloxacin, diclofenac and mefenamic acid in most of the canals and that of ciprofloxacin in the river, were greater than or close to 1, suggesting potential ecological risks. Ecological implications of the pharmaceutical residues in Bangkok waterway warrant further investigation. Copyright © 2012 Elsevier Ltd. All rights reserved.

Ozonation of oil sands process-affected water accelerates microbial bioremediation.

PubMed

Martin, Jonathan W; Barri, Thaer; Han, Xiumei; Fedorak, Phillip M; El-Din, Mohamed Gamal; Perez, Leonidas; Scott, Angela C; Jiang, Jason Tiange

2010-11-01

Ozonation can degrade toxic naphthenic acids (NAs) in oil sands process-affected water (OSPW), but even after extensive treatment a residual NA fraction remains. Here we hypothesized that mild ozonation would selectively oxidize the most biopersistent NA fraction, thereby accelerating subsequent NA biodegradation and toxicity removal by indigenous microbes. OSPW was ozonated to achieve approximately 50% and 75% NA degradation, and the major ozonation byproducts included oxidized NAs (i.e., hydroxy- or keto-NAs). However, oxidized NAs are already present in untreated OSPW and were shown to be formed during the microbial biodegradation of NAs. Ozonation alone did not affect OSPW toxicity, based on Microtox; however, there was a significant acceleration of toxicity removal in ozonated OSPW following inoculation with native microbes. Furthermore, all residual NAs biodegraded significantly faster in ozonated OSPW. The opposite trend was found for ozonated commercial NAs, which are known to contain no significant biopersistent fraction. Thus, we suggest that ozonation preferentially degraded the most biopersistent OSPW NA fraction, and that ozonation is complementary to the biodegradation capacity of microbial populations in OSPW. The toxicity of ozonated OSPW to higher organisms needs to be assessed, but there is promise that this technique could be applied to accelerate the bioremediation of large volumes of OSPW in Northern Alberta, Canada.

Authorization and Toxicity of Veterinary Drugs and Plant Protection Products: Residues of the Active Ingredients in Food and Feed and Toxicity Problems Related to Adjuvants

PubMed Central

Klátyik, Szandra; Bohus, Péter; Darvas, Béla; Székács, András

2017-01-01

Chemical substances applied in animal husbandry or veterinary medicine and in crop protection represent substantial environmental loads, and their residues occur in food and feed products. Product approval is governed differently in these two sectors in the European Union (EU), and the occurrence of veterinary drug (VD) and pesticide residues indicated by contamination notification cases in the Rapid Alert System for Food and Feed of the EU also show characteristic differences. While the initial high numbers of VD residues reported in 2002 were successfully suppressed to less than 100 cases annually by 2006 and on, the number of notification cases for pesticide residues showed a gradual increase from a low (approximately 50 cases annually) initial level until 2005 to more than 250 cases annually after 2009, with a halt occurring only in 2016. Main notifiers of VD residues include Germany, Belgium, the UK, and Italy (63, 59, 42, and 31 notifications announced, respectively), and main consigning countries of non-compliances are Vietnam, India, China, and Brazil (88, 50, 34, and 23 notifications, respectively). Thus, countries of South and Southeast Asia are considered a vulnerable point with regard to VD residues entering the EU market. Unintended side effects of VDs and plant protection products may be caused not only by the active ingredients but also by various additives in these preparations. Adjuvants (e.g., surfactants) and other co-formulants used in therapeutic agents and feed additives, as well as in pesticide formulations have long been considered as inactive ingredients in the aspects of the required main biological effect of the pharmaceutical or pesticide, and in turn, legal regulations of the approval and marketing of these additives specified significantly less stringent risk assessment requirements, than those specified for the active ingredients. However, numerous studies have shown additive, synergistic, or antagonistic side effects between the

Authorization and Toxicity of Veterinary Drugs and Plant Protection Products: Residues of the Active Ingredients in Food and Feed and Toxicity Problems Related to Adjuvants.

PubMed

Klátyik, Szandra; Bohus, Péter; Darvas, Béla; Székács, András

2017-01-01

Chemical substances applied in animal husbandry or veterinary medicine and in crop protection represent substantial environmental loads, and their residues occur in food and feed products. Product approval is governed differently in these two sectors in the European Union (EU), and the occurrence of veterinary drug (VD) and pesticide residues indicated by contamination notification cases in the Rapid Alert System for Food and Feed of the EU also show characteristic differences. While the initial high numbers of VD residues reported in 2002 were successfully suppressed to less than 100 cases annually by 2006 and on, the number of notification cases for pesticide residues showed a gradual increase from a low (approximately 50 cases annually) initial level until 2005 to more than 250 cases annually after 2009, with a halt occurring only in 2016. Main notifiers of VD residues include Germany, Belgium, the UK, and Italy (63, 59, 42, and 31 notifications announced, respectively), and main consigning countries of non-compliances are Vietnam, India, China, and Brazil (88, 50, 34, and 23 notifications, respectively). Thus, countries of South and Southeast Asia are considered a vulnerable point with regard to VD residues entering the EU market. Unintended side effects of VDs and plant protection products may be caused not only by the active ingredients but also by various additives in these preparations. Adjuvants (e.g., surfactants) and other co-formulants used in therapeutic agents and feed additives, as well as in pesticide formulations have long been considered as inactive ingredients in the aspects of the required main biological effect of the pharmaceutical or pesticide, and in turn, legal regulations of the approval and marketing of these additives specified significantly less stringent risk assessment requirements, than those specified for the active ingredients. However, numerous studies have shown additive, synergistic, or antagonistic side effects between the

Incorporation of carbohydrate residues into peroxidase isoenzymes in horseradish roots.

PubMed

Lew, J Y; Shannon, L M

1973-11-01

Sliced root tissue of the horseradish plant (Armoracia rusticana), when incubated with mannose-U-(14)C, incorporated radioactivity into peroxidase isoenzymes. Over 90% of the radioactivity in the highly purified peroxidase isoenzymes was present in the neutral sugar residues of the molecule, i.e. fucose, arabinose, xylose, mannose. When the root slices were incubated simultaneously with leucine-4,5-(3)H and mannose-U-(14)C, cycloheximide strongly inhibited leucine incorporation into the peptide portion of peroxidase isoenzymes but had little effect on the incorporation of (14)C into the neutral sugars. These results indicated that synthesis of the peptide portion of peroxidase was completed before the monosaccharide residues were attached to the molecule. This temporal relationship between the synthesis of protein and the attachment of carbohydrate residues in the plant glycoprotein, horseradish peroxidase, appears to be similar to that reported for glycoprotein biosynthesis in many mammalian systems.

Incorporation of Carbohydrate Residues into Peroxidase Isoenzymes in Horseradish Roots

PubMed Central

Lew, Jow Y.; Shannon, Leland M.

1973-01-01

Sliced root tissue of the horseradish plant (Armoracia rusticana), when incubated with mannose-U-14C, incorporated radioactivity into peroxidase isoenzymes. Over 90% of the radioactivity in the highly purified peroxidase isoenzymes was present in the neutral sugar residues of the molecule, i.e. fucose, arabinose, xylose, mannose. When the root slices were incubated simultaneously with leucine-4,5-3H and mannose-U-14C, cycloheximide strongly inhibited leucine incorporation into the peptide portion of peroxidase isoenzymes but had little effect on the incorporation of 14C into the neutral sugars. These results indicated that synthesis of the peptide portion of peroxidase was completed before the monosaccharide residues were attached to the molecule. This temporal relationship between the synthesis of protein and the attachment of carbohydrate residues in the plant glycoprotein, horseradish peroxidase, appears to be similar to that reported for glycoprotein biosynthesis in many mammalian systems. PMID:16658584

Peanut residue carbon and nitrogen mineralization under simulated conventional and conservation tillage

USDA-ARS?s Scientific Manuscript database

Residue management is an important aspect of crop production systems. Availability of plant residue nitrogen (N) to succeeding crops is dependent on N mineralization rates during decomposition. Cooperative Extension currently recommends 22-67 kg N ha-1 credit to subsequent crops following peanut (Ar...

Arctic Ocean Tides from GRACE Satellite Accelerations

NASA Astrophysics Data System (ADS)

Killett, B.; Wahr, J. M.; Desai, S. D.; Yuan, D.; Watkins, M. M.

2010-12-01

Because missions such as TOPEX/POSEIDON don't extend to high latitudes, Arctic ocean tidal solutions aren't constrained by altimetry data. The resulting errors in tidal models alias into monthly GRACE gravity field solutions at all latitudes. Fortunately, GRACE inter-satellite ranging data can be used to solve for these tides directly. Seven years of GRACE inter-satellite acceleration data are inverted using a mascon approach to solve for residual amplitudes and phases of major solar and lunar tides in the Arctic ocean relative to FES 2004. Simulations are performed to test the inversion algorithm's performance, and uncertainty estimates are derived from the tidal signal over land. Truncation error magnitudes and patterns are compared to the residual tidal signals.

Chemical modifications of therapeutic proteins induced by residual ethylene oxide.

PubMed

Chen, Louise; Sloey, Christopher; Zhang, Zhongqi; Bondarenko, Pavel V; Kim, Hyojin; Ren, Da; Kanapuram, Sekhar

2015-02-01

Ethylene oxide (EtO) is widely used in sterilization of drug product primary containers and medical devices. The impact of residual EtO on protein therapeutics is of significant interest in the biopharmaceutical industry. The potential for EtO to modify individual amino acids in proteins has been previously reported. However, specific identification of EtO adducts in proteins and the effect of residual EtO on the stability of therapeutic proteins has not been reported to date. This paper describes studies of residual EtO with two therapeutic proteins, a PEGylated form of the recombinant human granulocyte colony-stimulating factor (Peg-GCSF) and recombinant human erythropoietin (EPO) formulated with human serum albumin (HSA). Peg-GCSF was filled in an EtO sterilized delivery device and incubated at accelerated stress conditions. Glu-C peptide mapping and LC-MS analyses revealed residual EtO reacted with Peg-GCSF and resulted in EtO modifications at two methionine residues (Met-127 and Met-138). In addition, tryptic peptide mapping and LC-MS analyses revealed residual EtO in plastic vials reacted with HSA in EPO formulation at Met-328 and Cys-34. This paper details the work conducted to understand the effects of residual EtO on the chemical stability of protein therapeutics. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

Effects of crop residues of sunflower (Helianthus annuus), maize (Zea mays L.) and soybean (Glycine max) on growth and seed yields of sunflower.

PubMed

Srisa-Ard, K

2007-04-15

This pot experiment was carried out at Suranaree Technology University Experimental Farm, Northeast Thailand to investigate effects of crop residues of sunflower, maize and soybean on total dry weight, top dry weight, plant height, root dry weight and seed yield of sunflower plants with the use of Korat soil series (Oxic Paleustults) during the rainy season (July-October) of the 2001. The experiment was laid in a split plot arranged in a Completely Randomized Design (CRD) with four replications where the crop residues of maize, sunflower and soybean were used as main plots. Whilst crop residues of roots, top growth and roots+top growth were used as subplots. The results showed that crop residues derived from roots of both sunflower and soybean plants had their significant inhibition effects of allelopathic substances on plant height, root dry weight, top growth dry weight and total dry weight plant(-1) of the sunflower plants than those derived from top growth of both crops alone (sunflower and soybean). Maize plant residues had no significant inhibition effect on growth of subsequent crop of sunflower.

40 CFR 180.384 - Mepiquat (N,N-dimethylpip-eridinium); tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Mepiquat (N,N-dimethylpip-eridinium... Tolerances § 180.384 Mepiquat (N,N-dimethylpip-eridinium); tolerances for residues. (a) General. (1) Tolerances are established for residues of the plant growth regulator mepiquat (N,N-dimethylpiperidinium) in...

[Review on application of plant growth retardants in medicinal plants cultivation].

PubMed

Zhai, Yu-Yao; Guo, Bao-Lin; Cheng, Ming

2013-09-01

Plant growth retardants are widely used in cultivation of medicinal plant, but there is still lack of scientific guidance. In order to guide the use of plant growth retardants in medicinal plant cultivation efficiently and reasonably, this paper reviewed the mechanism, function characteristic, plant and soil residue of plant growth retardants, such as chlorocholine chloride, mepiquat chloride, paclobutrazol, unicnazle and succinic acid, and summarized the application of plant growth retardants in medicinal plants cultivation in recent years, with focus on the effect of growth and yield of the officinal organs and secondary metabolites.

Use of plant residues for improving soil fertility, pod nutrients, root growth and pod weight of okra (Abelmoschus esculentum L).

PubMed

Moyin-Jesu, Emmanuel Ibukunoluwa

2007-08-01

The effect of wood ash, sawdust, ground cocoa husk, spent grain and rice bran upon root development, ash content, pod yield and nutrient status and soil fertility for okra (Abelmoschus esculentum L NHAe 47 variety) was studied. The five organic fertilizer treatments were compared to chemical fertilizer (400kg/ha/crop NPK 15-15-15) and unfertilized controls in four field experiments replicated four times in a randomized complete block design. The results showed that the application of 6tha(-1) of plant residues increased (P<0.05) the soil N, P, K, Ca, Mg, pH, and SOM; pod N, P, K, Ca, Mg and ash; root length; and pod yield of okra in all four experiments relative to the control treatment. For instance, spent grain treatment increased the okra pod yield by 99%, 33%, 50%, 49%, 65% and 67% compared to control, NPK, wood ash, cocoa husk, rice bran and sawdust treatments respectively. In the stepwise regression, out of the total R(2) value of 0.83 for the soil nutrients to the pod yield of okra; soil N accounted for 50% of the soil fertility improvement and yield of okra. Spent grain, wood ash and cocoa husk were the most effective in improving okra pod weight, pod nutrients, ash content, root length and soil fertility whereas the rice bran and sawdust were the least effective. This was because the spent grain, wood ash and cocoa husk had lower C/N ratio and higher nutrient composition than rice bran and sawdust, thus, the former enhanced an increase in pod nutrients, composition for better human dietary intake, increased the root length, pod weight of okra and improved soil fertility and plant nutrition crop. The significance of the increases in okra mineral nutrition concentration by plant residues is that consumers will consume more of these minerals in their meals and monetarily spend less for purchasing vitamins and mineral supplement drugs to meet health requirements. In addition, the increase in plant nutrition and soil fertility would help to reduce the high cost

Transformation kinetics of corn and clover residues in mineral substrates of different composition

NASA Astrophysics Data System (ADS)

Pinskii, D. L.; Maltseva, A. N.; Zolotareva, B. N.; Dmitrieva, E. D.

2017-06-01

Mineralization kinetics of corn and clover residues in quartz sand, loam, sand + 15% bentonite, and sand + 30% kaolinite have been studied. A scheme has been proposed for the transformation of plant residues in mineral substrates. Kinetic parameters of mineralization have been calculated with the use of a first-order two-term exponential polynomial. It has been shown that the share of labile organic carbon pool in the clover biomass is higher (57-63%) than in the corn biomass (47-49%), which is related to the biochemical composition of plant residues. The mineralization constants of clover residues generally significantly exceed those of corn because of the stronger stabilization of the decomposition products of corn residues. The turnover time of the labile clover pool (4-9 days) in all substrates and that of the labile corn pool (8-10 days) in sands and substrates containing kaolinites and bentonite are typical for organic acids, amino acids, and simple sugars. In the loamy substrate, the turnover time of labile corn pool is about 46 days due to the stronger stabilization of components of the labile pool containing large amounts of organic acids. The turnover time of the stable clover pool (0.95 years) is significantly lower than that of the stable corn pool (1.60 years) and largely corresponds to the turnover time of plant biomass.

40 CFR 180.325 - 2-(m-Chlorophenoxy) propionic acid; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 23 2010-07-01 2010-07-01 false 2-(m-Chlorophenoxy) propionic acid... Tolerances § 180.325 2-(m-Chlorophenoxy) propionic acid; tolerances for residues. (a) General. A tolerance is established for negligible residues of the plant regulator 2-(m-chlorophenoxy) propionic acid from application...

Adsorption Property and Mechanism of Oxytetracycline onto Willow Residues

PubMed Central

Wang, Di; Xu, Haiyang; Yang, Shengke; Wang, Wenke; Wang, Yanhua

2017-01-01

To elucidate the adsorption property and the mechanism of plant residues to reduce oxytetracycline (OTC), the adsorption of OTC onto raw willow roots (WR-R), stems (WS-R), leaves (WL-R), and adsorption onto desugared willow roots (WR-D), stems (WS-D), and leaves (WL-D) were investigated. The structural characterization was analyzed by scanning electron microscopy, Fourier-transform infrared spectra, and an elemental analyzer. OTC adsorption onto the different tissues of willow residues was compared and correlated with their structures. The adsorption kinetics of OTC onto willow residues was found to follow the pseudo-first-order model. The isothermal adsorption process of OTC onto the different tissues of willow residues followed the Langmuir and Freundlich model and the process was also a spontaneous endothermic reaction, which was mainly physical adsorption. After the willow residues were desugared, the polarity decreased and the aromaticity increased, which explained why the adsorption amounts of the desugared willow residues were higher than those of the unmodified residues. These observations suggest that the raw and modified willow residues have great potential as adsorbents to remove organic pollutants. PMID:29271892

Use of filler limestone and construction and demolition residues for remediating soils contaminated with heavy metals: an assessment by means of plant uptake.

NASA Astrophysics Data System (ADS)

Banegas, Ascension; Martinez-Sanchez, Maria Jose; Agudo, Ines; Perez-Sirvent, Carmen

2010-05-01

A greenhouse trial was carried out to evaluate the assimilation of heavy metals by three types of horticultural plants (lettuce, broccoli and alfalfa), different parts of which are destined for human and animal consumption (leaves, roots, fruits). The plants were cultivated in four types of soil, one uncontaminated (T1), one soil collected in the surrounding area of Sierra Minera (T2), the third being remediated with residues coming from demolition and construction activities (T3) and the four remediated with filler limestone (T4). To determine the metal content, soil samples were first ground to a fine powder using an agate ball mill. Fresh vegetable samples were separated into root and aboveground biomass and then lyophilized. The DTPA-extractable content was also determined to calculate the bioavailable amount of metal. Finally, the translocation factor (TF) and bioconcentration factor (BCF) were calculated. Arsenic levels were obtained by using atomic fluorescence spectrometry with an automated continuous flow hydride generation (HG-AFS) spectrometer and Cd, Pb and Zn was determined by electrothermal atomization atomic absorption spectrometry (ETAAS) or flame atomic absorption spectrometry (FAAS). Samples of the leached water were also obtained and analyzed. According to our results, the retention of the studied elements varies with the type of plant and is strongly decreased by the incorporation of filler limestone and/or construction and demolition residues to the soils. This practice represents a suitable way to reduce the risk posed to the biota by the presence of high levels of heavy metal in soil.

Non-destructive measurement and role of surface residual stress monitoring in residual life assessment of a steam turbine blading material

NASA Astrophysics Data System (ADS)

Prabhu-Gaunkar, Gajanana; Rawat, M. S.; Prasad, C. R.

2014-02-01

Steam turbine blades in power generation equipment are made from martensitic stainless steels having high strength, good toughness and corrosion resistance. However, these steels are susceptible to pitting which can promote early failures of blades in the turbines, particularly in the low pressure dry/wet areas by stress corrosion and corrosion fatigue. Presence of tensile residual stresses is known to accelerate failures whereas compressive stresses can help in delaying failures. Shot peening has been employed as an effective tool to induce compressive residual stresses which offset a part of local surface tensile stresses in the surface layers of components. Maintaining local stresses at stress raisers, such as pits formed during service, below a threshold level can help in preventing the initiation microcracks and failures. The thickness of the layer in compression will, however, depend of the shot peening parameters and should extend below the bottom of corrosion pits. The magnitude of surface compressive drops progressively during service exposure and over time the effectiveness of shot peening is lost making the material susceptible to micro-crack initiation once again. Measurement and monitoring of surface residual stress therefore becomes important for assessing residual life of components in service. This paper shows the applicability of surface stress monitoring to life assessment of steam turbine blade material based on data generated in laboratory on residual surface stress measurements in relation to fatigue exposure. An empirical model is proposed to calculate the remaining life of shot peened steam turbine blades in service.

The three-dimensional structure of AKR11B4, a glycerol dehydrogenase from Gluconobacter oxydans, reveals a tryptophan residue as an accelerator of reaction turnover.

PubMed

Richter, Nina; Breicha, Klaus; Hummel, Werner; Niefind, Karsten

2010-12-03

The NADP-dependent glycerol dehydrogenase (EC 1.1.1.72) from Gluconobacter oxydans is a member of family 11 of the aldo-keto reductase (AKR) enzyme superfamily; according to the systematic nomenclature within the AKR superfamily, the term AKR11B4 has been assigned to the enzyme. AKR11B4 is a biotechnologically attractive enzyme because of its broad substrate spectrum, combined with its distinctive regioselectivity and stereoselectivity. These features can be partially rationalized based on a 2-Å crystal structure of apo-AKR11B4, which we describe and interpret here against the functional complex structures of other members of family 11 of the AKR superfamily. The structure of AKR11B4 shows the AKR-typical (β/α)(8) TIM-barrel fold, with three loops and the C-terminal tail determining the particular enzymatic properties. In comparison to AKR11B1 (its closest AKR relative), AKR11B4 has a relatively broad binding cleft for the cosubstrate NADP/NADPH. In the crystalline environment, it is completely blocked by the C-terminal segment of a neighboring protomer. The structure reveals a conspicuous tryptophan residue (Trp23) that has to adopt an unconventional and strained side-chain conformation to permit cosubstrate binding. We predict and confirm by site-directed mutagenesis that Trp23 is an accelerator of (co)substrate turnover. Furthermore, we show that, simultaneously, this tryptophan residue is a critical determinant for substrate binding by the enzyme, while enantioselectivity is probably governed by a methionine residue within the C-terminal tail. We present structural reasons for these notions based on ternary complex models of AKR11B4, NADP, and either octanal, d-glyceraldehyde, or l-glyceraldehyde. Copyright © 2010 Elsevier Ltd. All rights reserved.

Multilevel acceleration of scattering-source iterations with application to electron transport

DOE PAGES

Drumm, Clif; Fan, Wesley

2017-08-18

Acceleration/preconditioning strategies available in the SCEPTRE radiation transport code are described. A flexible transport synthetic acceleration (TSA) algorithm that uses a low-order discrete-ordinates (S N) or spherical-harmonics (P N) solve to accelerate convergence of a high-order S N source-iteration (SI) solve is described. Convergence of the low-order solves can be further accelerated by applying off-the-shelf incomplete-factorization or algebraic-multigrid methods. Also available is an algorithm that uses a generalized minimum residual (GMRES) iterative method rather than SI for convergence, using a parallel sweep-based solver to build up a Krylov subspace. TSA has been applied as a preconditioner to accelerate the convergencemore » of the GMRES iterations. The methods are applied to several problems involving electron transport and problems with artificial cross sections with large scattering ratios. These methods were compared and evaluated by considering material discontinuities and scattering anisotropy. Observed accelerations obtained are highly problem dependent, but speedup factors around 10 have been observed in typical applications.« less

Managing woodwaste: Yield from residue

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nielson, E.; Rayner, S.

1993-12-31

Historically, the majority of sawmill waste has been burned or buried for the sole purpose of disposal. In most jurisdictions, environmental legislation will prohibit, or render uneconomic, these practices. Many reports have been prepared to describe the forest industry`s residue and its environmental effect; although these help those looking for industry-wide or regional solutions, such as electricity generation, they have limited value for the mill manager, who has the on-hands responsibility for generation and disposal of the waste. If the mill manager can evaluate waste streams and break them down into their usable components, he can find niche market solutionsmore » for portions of the plant residue and redirect waste to poor/no-return, rather than disposal-cost, end uses. In the modern mill, residue is collected at the individual machine centre by waste conveyors that combine and mix sawdust, shavings, bark, etc. and send the result to the hog-fuel pile. The mill waste system should be analyzed to determine the measures that can improve the quality of residues and determine the volumes of any particular category before the mixing, mentioned above, occurs. After this analysis, the mill may find a niche market for a portion of its woodwaste.« less

Dissipation rates and residues of fungicide azoxystrobin in ginseng and soil at two different cultivated regions in China.

PubMed

Hou, Zhiguang; Wang, Xiumei; Zhao, Xiaofeng; Wang, Xinhong; Yuan, Xing; Lu, Zhongbin

2016-07-01

The maximum residue limit (MRL) for fungicide azoxystrobin in ginseng has not yet been established in China. This is partially due to the lack of its dissipation and residue data at China's main ginseng production areas. In this work, the dissipation rates and residue levels of azoxystrobin in ginseng roots, plant parts (stems and leaves), and soil in Beijing and Jilin Province, China were determined using gas chromatograph-mass spectrometry (GC-MS). The mean half-life of azoxystrobin in ginseng plant parts was 1.6 days with a dissipation rate of 90 % over 21 days. The mean half-life in soil was 2.8 days with a dissipation rate of 90 % over 30 days. Dissipation rates from two geographically separated experimental fields differed, suggesting that these were affected by local soil characteristics and climate. Maximum final residues of azoxystrobin in ginseng roots, plant parts, and soil were determined to be 0.343, 9.40, and 0.726 mg kg(-1), respectively. Our results, particularly the high residues of azoxystrobin observed in ginseng plant parts, provide a quantitative basis for revising the application of this pesticide to ginseng.

The Importance of Residual Kidney Function in Haemodialysis Patients.

PubMed

Kong, Jessica; Davies, Matthew; Mount, Peter

2018-06-19

In contrast to peritoneal dialysis, residual kidney function is commonly disregarded for haemodialysis patients, and not regularly monitored or taken into account in routine clinical care. This is despite evidence that higher levels of residual kidney function in haemodialysis patients associate with better outcomes including survival, total solute clearance, nutrition, inflammation, and fluid balance. This review aims to summarise the clinical effects of residual kidney function specifically in haemodialysis patients. Some level of residual kidney function is present in over 80% of patients at the time of dialysis initiation, and while this declines over time, up to 30% of patients on haemodialysis for 5 years still have a measurable level of native kidney function. There is little evidence on how best to preserve residual kidney function in haemodialysis patients, although it has been observed that intensive haemodialysis regimens in incident haemodialysis patients appear to accelerate residual kidney function decline. Residual kidney function is not commonly factored in to haemodialysis prescription and measures of adequacy, despite the fact that some guidelines such as KDOQI and European Best Practice Guidelines suggest that it is reasonable to do. This likely relates, at least in part, to perceived concerns regarding the inconvenience of timed urine collections, and to the complexity and lack of consensus regarding the methods for integrating the intermittent clearance of haemodialysis with the continuous clearance of native renal function. Further research is required into how best to maintain and maximise the benefits of residual kidney function in haemodialysis patients. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Multistep food plant processing at Grotta Paglicci (Southern Italy) around 32,600 cal B.P.

PubMed Central

Mariotti Lippi, Marta; Foggi, Bruno; Aranguren, Biancamaria; Ronchitelli, Annamaria; Revedin, Anna

2015-01-01

Residue analyses on a grinding tool recovered at Grotta Paglicci sublayer 23A [32,614 ± 429 calibrated (cal) B.P.], Southern Italy, have demonstrated that early modern humans collected and processed various plants. The recording of starch grains attributable to Avena (oat) caryopses expands our information about the food plants used for producing flour in Europe during the Paleolithic and about the origins of a food tradition persisting up to the present in the Mediterranean basin. The quantitative distribution of the starch grains on the surface of the grinding stone furnished information about the tool handling, confirming its use as a pestle-grinder, as suggested by the wear-trace analysis. The particular state of preservation of the starch grains suggests the use of a thermal treatment before grinding, possibly to accelerate drying of the plants, making the following process easier and faster. The study clearly indicates that the exploitation of plant resources was very important for hunter–gatherer populations, to the point that the Early Gravettian inhabitants of Paglicci were able to process food plants and already possessed a wealth of knowledge that was to become widespread after the dawn of agriculture. PMID:26351674

Multistep food plant processing at Grotta Paglicci (Southern Italy) around 32,600 cal B.P.

PubMed

Mariotti Lippi, Marta; Foggi, Bruno; Aranguren, Biancamaria; Ronchitelli, Annamaria; Revedin, Anna

2015-09-29

Residue analyses on a grinding tool recovered at Grotta Paglicci sublayer 23A [32,614 ± 429 calibrated (cal) B.P.], Southern Italy, have demonstrated that early modern humans collected and processed various plants. The recording of starch grains attributable to Avena (oat) caryopses expands our information about the food plants used for producing flour in Europe during the Paleolithic and about the origins of a food tradition persisting up to the present in the Mediterranean basin. The quantitative distribution of the starch grains on the surface of the grinding stone furnished information about the tool handling, confirming its use as a pestle-grinder, as suggested by the wear-trace analysis. The particular state of preservation of the starch grains suggests the use of a thermal treatment before grinding, possibly to accelerate drying of the plants, making the following process easier and faster. The study clearly indicates that the exploitation of plant resources was very important for hunter-gatherer populations, to the point that the Early Gravettian inhabitants of Paglicci were able to process food plants and already possessed a wealth of knowledge that was to become widespread after the dawn of agriculture.

A beamline systems model for Accelerator-Driven Transmutation Technology (ADTT) facilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Todd, A.M.M.; Paulson, C.C.; Peacock, M.A.

1995-10-01

A beamline systems code, that is being developed for Accelerator-Driven Transmutation Technology (ADTT) facility trade studies, is described. The overall program is a joint Grumman, G.H. Gillespie Associates (GHGA) and Los Alamos National Laboratory effort. The GHGA Accelerator Systems Model (ASM) has been adopted as the framework on which this effort is based. Relevant accelerator and beam transport models from earlier Grumman systems codes are being adapted to this framework. Preliminary physics and engineering models for each ADTT beamline component have been constructed. Examples noted include a Bridge Coupled Drift Tube Linac (BCDTL) and the accelerator thermal system. A decisionmore » has been made to confine the ASM framework principally to beamline modeling, while detailed target/blanket, balance-of-plant and facility costing analysis will be performed externally. An interfacing external balance-of-plant and facility costing model, which will permit the performance of iterative facility trade studies, is under separate development. An ABC (Accelerator Based Conversion) example is used to highlight the present models and capabilities.« less

A beamline systems model for Accelerator-Driven Transmutation Technology (ADTT) facilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Todd, Alan M. M.; Paulson, C. C.; Peacock, M. A.

1995-09-15

A beamline systems code, that is being developed for Accelerator-Driven Transmutation Technology (ADTT) facility trade studies, is described. The overall program is a joint Grumman, G. H. Gillespie Associates (GHGA) and Los Alamos National Laboratory effort. The GHGA Accelerator Systems Model (ASM) has been adopted as the framework on which this effort is based. Relevant accelerator and beam transport models from earlier Grumman systems codes are being adapted to this framework. Preliminary physics and engineering models for each ADTT beamline component have been constructed. Examples noted include a Bridge Coupled Drift Tube Linac (BCDTL) and the accelerator thermal system. Amore » decision has been made to confine the ASM framework principally to beamline modeling, while detailed target/blanket, balance-of-plant and facility costing analysis will be performed externally. An interfacing external balance-of-plant and facility costing model, which will permit the performance of iterative facility trade studies, is under separate development. An ABC (Accelerator Based Conversion) example is used to highlight the present models and capabilities.« less

Sugarcane field residue and bagasse allelopathic impact on vegetable seed germination

USDA-ARS?s Scientific Manuscript database

The chemical interaction between plants, which is referred to as allelopathy, may result in the inhibition of plant growth and development. The objective of this research was to determine the allelopathic impact of sugarcane (Saccharum officinarum) var. ‘HoCP 96-540’ field residue and sugarcane baga...

Effects of alternate fuels. Report No. 2. Analysis of basic refractories degraded by residual oil combustion products

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wei, G. C.; Tennery, V. J.

1978-02-01

Industrial conversion in the U.S. to alternate fuels from natural gas is presently under way and will accelerate rapidly as a result of gas curtailments and National policy considerations. Currently the prime alternate fuels are distillate and residual oils and coal. Conversion to residual oils or coal for high-temperature process heat applications is anticipated to result in accelerated refractory and insulation corrosion and degradation due to reactions between fuel impurities and the ceramic linings of high-temperature equipment. Understanding the nature of such reactions and identification of means for preventing or retarding them will be of considerable assistance to both refractorymore » manufacturers and users as well as a significant contribution to energy conservation.« less

Investigation of the residue in an electric rail gun employing a plasma armature

NASA Technical Reports Server (NTRS)

Bauer, D. P.; Barber, J. P.

1984-01-01

The performance of dc electric rail guns using plasma-armature-accelerated projectiles was studied. It was found that the initial rail launcher acceleration profile was consistent with the simulation, but that after the projectile had traveled approximately 25 to 30 cm along the gun, a considerable portion of the current in the projectile armature commutated into a secondary current path. Also noted were the lower than expected muzzle velocities. It was proposed that the secondary current path was a relatively high conductivity layer of residue on the launcher bore.

Importance of residual primary cancer after induction therapy for esophageal adenocarcinoma.

PubMed

Raja, Siva; Rice, Thomas W; Ehrlinger, John; Goldblum, John R; Rybicki, Lisa A; Murthy, Sudish C; Adelstein, David; Videtic, Gregory; McNamara, Michael P; Blackstone, Eugene H

2016-09-01

To (1) assess the continuous distribution of the percentage of residual primary cancer in resection specimens after induction therapy for locally advanced esophageal adenocarcinoma, (2) determine the effects of residual primary cancer on survival after esophagectomy, (3) ascertain interplay between residual primary cancer and classical classifications of response to induction therapy (ypTNM), and (4) identify predictors of residual primary cancer. From January 2006 to November 2012, 188 patients (78%) underwent accelerated chemoradiotherapy, and 52 patients (22%) underwent chemotherapy alone followed by esophagectomy for adenocarcinoma. Mean age was 61 ± 9.2 years, and 89% were male. Residual primary cancer, assessed as the percentage of residual primary cancer cells in resection specimens, was quantified histologically by a gastrointestinal pathologist. Random Forest technology was used for data analysis. Twenty-five specimens (10%) had no residual primary cancer (ypT0), 79 (33%) had 1% to 25% residual cancer, 91 (38%) had 26% to 75%, and 45 (19%) had >75%. Survival was worse with increasing residual primary cancer, plateauing at 75%. Greater residual primary cancer was associated with worse survival across the spectrum of higher ypTN. Higher ypT, larger number of positive nodes, and use of induction chemotherapy rather than induction chemoradiotherapy were associated with greater residual primary cancer. Less residual primary cancer in response to preoperative therapy is associated with a linear increase in survival after esophagectomy for locally advanced esophageal adenocarcinoma; however, survival is poorer than for resected early-stage cancers. Therefore, for patients with poor prognostic indicators, including higher percentage of residual primary cancer, the role of adjuvant therapy needs to be further examined in an attempt to improve survival. Copyright © 2016. Published by Elsevier Inc.

The effectiveness of sewage treatment processes to remove faecal pathogens and antibiotic residues

PubMed Central

Hendricks, Rahzia; Pool, Edmund John

2012-01-01

Pathogens and antibiotics enter the aquatic environment via sewage effluents and may pose a health risk to wild life and humans. The aim of this study was to determine the levels of faecal bacteria, and selected antibiotic residues in raw wastewater and treated sewage effluents from three different sewage treatment plants in the Western Cape, South Africa. Sewage treatment plant 1 and 2 use older technologies, while sewage treatment plant 3 has been upgraded and membrane technologies were incorporated in the treatment processes. Coliforms and Escherichia coli (E. coli) were used as bioindicators for faecal bacteria. A chromogenic test was used to screen for coliforms and E. coli. Fluoroquinolones and sulfamethoxazole are commonly used antibiotics and were selected to monitor the efficiency of sewage treatment processes for antibiotic removal. Enzyme Linked Immunosorbent Assays (ELISAs) were used to quantitate antibiotic residues in raw and treated sewage. Raw intake water at all treatment plants contained total coliforms and E. coli. High removal of E. coli by treatment processes was evident for treatment plant 2 and 3 only. Fluoroquinolones and sulfamethoxazole were detected in raw wastewater from all sewage treatment plants. Treatment processes at plant 1 did not reduce the fluoroquinolone concentration in treated sewage effluents. Treatment processes at plant 2 and 3 reduced the fluoroquinolone concentration by 21% and 31%, respectively. Treatment processes at plant 1 did not reduce the sulfamethoxazole concentration in treated sewage effluents. Treatment processes at plant 2 and 3 reduced sulfamethoxazole by 34% and 56%, respectively. This study showed that bacteria and antibiotic residues are still discharged into the environment. Further research needs to be undertaken to improve sewage treatment technologies, thereby producing a better quality treated sewage effluent. PMID:22242882

Dynamic Response and Residual Helmet Liner Crush Using Cadaver Heads and Standard Headforms.

PubMed

Bonin, S J; Luck, J F; Bass, C R; Gardiner, J C; Onar-Thomas, A; Asfour, S S; Siegmund, G P

2017-03-01

Biomechanical headforms are used for helmet certification testing and reconstructing helmeted head impacts; however, their biofidelity and direct applicability to human head and helmet responses remain unclear. Dynamic responses of cadaver heads and three headforms and residual foam liner deformations were compared during motorcycle helmet impacts. Instrumented, helmeted heads/headforms were dropped onto the forehead region against an instrumented flat anvil at 75, 150, and 195 J. Helmets were CT scanned to quantify maximum liner crush depth and crush volume. General linear models were used to quantify the effect of head type and impact energy on linear acceleration, head injury criterion (HIC), force, maximum liner crush depth, and liner crush volume and regression models were used to quantify the relationship between acceleration and both maximum crush depth and crush volume. The cadaver heads generated larger peak accelerations than all three headforms, larger HICs than the International Organization for Standardization (ISO), larger forces than the Hybrid III and ISO, larger maximum crush depth than the ISO, and larger crush volumes than the DOT. These significant differences between the cadaver heads and headforms need to be accounted for when attempting to estimate an impact exposure using a helmet's residual crush depth or volume.

Multi-residue analysis of pesticides, plant hormones, veterinary drugs and mycotoxins using HILIC chromatography - MS/MS in various food matrices.

PubMed

Danezis, G P; Anagnostopoulos, C J; Liapis, K; Koupparis, M A

2016-10-26

One of the recent trends in Analytical Chemistry is the development of economic, quick and easy hyphenated methods to be used in a field that includes analytes of different classes and physicochemical properties. In this work a multi-residue method was developed for the simultaneous determination of 28 xenobiotics (polar and hydrophilic) using hydrophilic interaction liquid chromatography technique (HILIC) coupled with triple quadrupole mass spectrometry (LC-MS/MS) technology. The scope of the method includes plant growth regulators (chlormequat, daminozide, diquat, maleic hydrazide, mepiquat, paraquat), pesticides (cyromazine, the metabolite of the fungicide propineb PTU (propylenethiourea), amitrole), various multiclass antibiotics (tetracyclines, sulfonamides quinolones, kasugamycin and mycotoxins (aflatoxin B1, B2, fumonisin B1 and ochratoxin A). Isolation of the analytes from the matrix was achieved with a fast and effective technique. The validation of the multi-residue method was performed at the levels: 10 μg/kg and 100 μg/kg in the following representative substrates: fruits-vegetables (apples, apricots, lettuce and onions), cereals and pulses (flour and chickpeas), animal products (milk and meat) and cereal based baby foods. The method was validated taking into consideration EU guidelines and showed acceptable linearity (r ≥ 0.99), accuracy with recoveries between 70 and 120% and precision with RSD ≤ 20% for the majority of the analytes studied. For the analytes that presented accuracy and precision values outside the acceptable limits the method still is able to serve as a semi-quantitative method. The matrix effect, the limits of detection and quantification were also estimated and compared with the current EU MRLs (Maximum Residue Levels) and FAO/WHO MLs (Maximum Levels) or CXLs (Codex Maximum Residue Limits). The combined and expanded uncertainty of the method for each analyte per substrate, was also estimated. Copyright © 2016 Elsevier B

Combining Landsat-8 and WorldView-3 data to assess crop residue cover

USDA-ARS?s Scientific Manuscript database

Crop residues on the soil surface contribute to soil quality and form the first line defense against the erosive forces of water and wind. Quantifying crop residue cover on the soil surface after crops are planted is crucial for monitoring soil tillage intensity and assessing the extent of conserva...

Field Studies to Evaluate Potential Differences between Bt and non-Bt Corn Residue

USDA-ARS?s Scientific Manuscript database

Some reports suggest that the genetically-modified Bt corn residue may have higher lignin content and that the residue may be more resistant to decomposition. If true, then there are implications for both farming practices, e.g., tillage and planting, as well as global carbon budgets. We conducted ...

Field Studies to Evaluate Potential Differences between Bt and non-Bt Corn Residue

USDA-ARS?s Scientific Manuscript database

Some reports suggest that the genetically-modified Bt corn residue may have higher lignin content and that the residue may be more resistant to decomposition. If true, then there are implications for both farming practices, e.g., tillage and planting, as well as global carbon budgets. We evaluated ...

Toward GPGPU accelerated human electromechanical cardiac simulations

PubMed Central

Vigueras, Guillermo; Roy, Ishani; Cookson, Andrew; Lee, Jack; Smith, Nicolas; Nordsletten, David

2014-01-01

In this paper, we look at the acceleration of weakly coupled electromechanics using the graphics processing unit (GPU). Specifically, we port to the GPU a number of components of Heart—a CPU-based finite element code developed for simulating multi-physics problems. On the basis of a criterion of computational cost, we implemented on the GPU the ODE and PDE solution steps for the electrophysiology problem and the Jacobian and residual evaluation for the mechanics problem. Performance of the GPU implementation is then compared with single core CPU (SC) execution as well as multi-core CPU (MC) computations with equivalent theoretical performance. Results show that for a human scale left ventricle mesh, GPU acceleration of the electrophysiology problem provided speedups of 164 × compared with SC and 5.5 times compared with MC for the solution of the ODE model. Speedup of up to 72 × compared with SC and 2.6 × compared with MC was also observed for the PDE solve. Using the same human geometry, the GPU implementation of mechanics residual/Jacobian computation provided speedups of up to 44 × compared with SC and 2.0 × compared with MC. © 2013 The Authors. International Journal for Numerical Methods in Biomedical Engineering published by John Wiley & Sons, Ltd. PMID:24115492

Ornamental plants on sale to the public are a significant source of pesticide residues with implications for the health of pollinating insects.

PubMed

Lentola, A; David, A; Abdul-Sada, A; Tapparo, A; Goulson, D; Hill, E M

2017-09-01

Garden centres frequently market nectar- and pollen-rich ornamental plants as "pollinator-friendly", however these plants are often treated with pesticides during their production. There is little information on the nature of pesticide residues present at the point of purchase and whether these plants may actually pose a threat to, rather than benefit, the health of pollinating insects. Using mass spectrometry analyses, this study screened leaves from 29 different 'bee-friendly' plants for 8 insecticides and 16 fungicides commonly used in ornamental production. Only two plants (a Narcissus and a Salvia variety) did not contain any pesticide and 23 plants contained more than one pesticide, with some species containing mixtures of 7 (Ageratum houstonianum) and 10 (Erica carnea) different agrochemicals. Neonicotinoid insecticides were detected in more than 70% of the analysed plants, and chlorpyrifos and pyrethroid insecticides were found in 10% and 7% of plants respectively. Boscalid, spiroxamine and DMI-fungicides were detected in 40% of plants. Pollen samples collected from 18 different plants contained a total of 13 different pesticides. Systemic compounds were detected in pollen samples at similar concentrations to those in leaves. However, some contact (chlorpyrifos) and localised penetrant pesticides (iprodione, pyroclastrobin and prochloraz) were also detected in pollen, likely arising from direct contamination during spraying. The neonicotinoids thiamethoxam, clothianidin and imidacloprid and the organophosphate chlorpyrifos were present in pollen at concentrations between 6.9 and 81 ng/g and at levels that overlap with those known to cause harm to bees. The net effect on pollinators of buying plants that are a rich source of forage for them but simultaneously risk exposing them to a cocktail of pesticides is not clear. Gardeners who wish to gain the benefits without the risks should seek uncontaminated plants by growing their own from seed, plant

Continuous estimates on the earthquake early warning magnitude by use of the near-field acceleration records

NASA Astrophysics Data System (ADS)

Li, Jun; Jin, Xing; Wei, Yongxiang; Zhang, Hongcai

2013-10-01

In this article, the seismic records of Japan's Kik-net are selected to measure the acceleration, displacement, and effective peak acceleration of each seismic record within a certain time after P wave, then a continuous estimation is given on earthquake early warning magnitude through statistical analysis method, and Wenchuan earthquake record is utilized to check the method. The results show that the reliability of earthquake early warning magnitude continuously increases with the increase of the seismic information, the biggest residual happens if the acceleration is adopted to fit earthquake magnitude, which may be caused by rich high-frequency components and large dispersion of peak value in acceleration record, the influence caused by the high-frequency components can be effectively reduced if the effective peak acceleration and peak displacement is adopted, it is estimated that the dispersion of earthquake magnitude obviously reduces, but it is easy for peak displacement to be affected by long-period drifting. In various components, the residual enlargement phenomenon at vertical direction is almost unobvious, thus it is recommended in this article that the effective peak acceleration at vertical direction is preferred to estimate earthquake early warning magnitude. Through adopting Wenchuan strong earthquake record to check the method mentioned in this article, it is found that this method can be used to quickly, stably, and accurately estimate the early warning magnitude of this earthquake, which shows that this method is completely applicable for earthquake early warning.

Long-term residual dry matter mapping for monitoring California hardwood rangelands

Treesearch

Norman R. Harris; William E. Frost; Neil K. McDougald; Melvin R. George; Donald L. Nielsen

2002-01-01

Long-term residual dry matter mapping on the San Joaquin Experimental Range provides a working example of this monitoring technique for grazing management and research. Residual dry matter (RDM) is the amount of old plant material left on the ground at the beginning of a new growing season. RDM indicates the previous season’s use and can be used to describe the health...

Electrodialytic remediation of municipal solid waste incineration residues using different membranes.

PubMed

Parés Viader, Raimon; Jensen, Pernille Erland; Ottosen, Lisbeth M

2017-02-01

In the present work, three different commercial membrane brands were used in an identical electrodialytic cell setup and operating conditions, in order to reduce the leaching of metals and salt anions of two types of municipal solid waste incineration residues: air pollution control residues of a semi-dry flue-gas cleaning system and fly ashes from a plant with wet flue-gas cleaning system. The results showed a general reduction of the leaching in both residues after ED remediation. For the following elements, the leaching was found to be different after ED treatment depending on the membrane used, with statistical significance: • Air pollution control residues of the semi-dry flue-gas cleaning system: Cr, Cu, Ni, Pb, Zn; • Fly ashes from a plant with wet flue-gas cleaning system: Al, Ba, Cu, Ni, Zn, Cl, SO 4 . Final leaching values for some elements and membranes, but not the majority, were below than those of certified coal fly ash (e.g. Al or Cr), a material which is commonly used in construction materials; at the same time, some of these values were reduced to below the Danish law thresholds on the use of contaminated soil in constructions. These results show the potential of ED as a technology to upgrade municipal solid waste incineration residues. Copyright © 2016 Elsevier Ltd. All rights reserved.

Genomic Prediction Accounting for Residual Heteroskedasticity.

PubMed

Ou, Zhining; Tempelman, Robert J; Steibel, Juan P; Ernst, Catherine W; Bates, Ronald O; Bello, Nora M

2015-11-12

Whole-genome prediction (WGP) models that use single-nucleotide polymorphism marker information to predict genetic merit of animals and plants typically assume homogeneous residual variance. However, variability is often heterogeneous across agricultural production systems and may subsequently bias WGP-based inferences. This study extends classical WGP models based on normality, heavy-tailed specifications and variable selection to explicitly account for environmentally-driven residual heteroskedasticity under a hierarchical Bayesian mixed-models framework. WGP models assuming homogeneous or heterogeneous residual variances were fitted to training data generated under simulation scenarios reflecting a gradient of increasing heteroskedasticity. Model fit was based on pseudo-Bayes factors and also on prediction accuracy of genomic breeding values computed on a validation data subset one generation removed from the simulated training dataset. Homogeneous vs. heterogeneous residual variance WGP models were also fitted to two quantitative traits, namely 45-min postmortem carcass temperature and loin muscle pH, recorded in a swine resource population dataset prescreened for high and mild residual heteroskedasticity, respectively. Fit of competing WGP models was compared using pseudo-Bayes factors. Predictive ability, defined as the correlation between predicted and observed phenotypes in validation sets of a five-fold cross-validation was also computed. Heteroskedastic error WGP models showed improved model fit and enhanced prediction accuracy compared to homoskedastic error WGP models although the magnitude of the improvement was small (less than two percentage points net gain in prediction accuracy). Nevertheless, accounting for residual heteroskedasticity did improve accuracy of selection, especially on individuals of extreme genetic merit. Copyright © 2016 Ou et al.

Genomic Prediction Accounting for Residual Heteroskedasticity

PubMed Central

Ou, Zhining; Tempelman, Robert J.; Steibel, Juan P.; Ernst, Catherine W.; Bates, Ronald O.; Bello, Nora M.

2015-01-01

Whole-genome prediction (WGP) models that use single-nucleotide polymorphism marker information to predict genetic merit of animals and plants typically assume homogeneous residual variance. However, variability is often heterogeneous across agricultural production systems and may subsequently bias WGP-based inferences. This study extends classical WGP models based on normality, heavy-tailed specifications and variable selection to explicitly account for environmentally-driven residual heteroskedasticity under a hierarchical Bayesian mixed-models framework. WGP models assuming homogeneous or heterogeneous residual variances were fitted to training data generated under simulation scenarios reflecting a gradient of increasing heteroskedasticity. Model fit was based on pseudo-Bayes factors and also on prediction accuracy of genomic breeding values computed on a validation data subset one generation removed from the simulated training dataset. Homogeneous vs. heterogeneous residual variance WGP models were also fitted to two quantitative traits, namely 45-min postmortem carcass temperature and loin muscle pH, recorded in a swine resource population dataset prescreened for high and mild residual heteroskedasticity, respectively. Fit of competing WGP models was compared using pseudo-Bayes factors. Predictive ability, defined as the correlation between predicted and observed phenotypes in validation sets of a five-fold cross-validation was also computed. Heteroskedastic error WGP models showed improved model fit and enhanced prediction accuracy compared to homoskedastic error WGP models although the magnitude of the improvement was small (less than two percentage points net gain in prediction accuracy). Nevertheless, accounting for residual heteroskedasticity did improve accuracy of selection, especially on individuals of extreme genetic merit. PMID:26564950

Risk Assessment Document for Coke Oven MACT Residual Risk

EPA Pesticide Factsheets

The residual risk analysis described in this report addresses four coke plants subject to the 1993coke oven MACT standards (40 CFR Part 63 Subpart L) and estimates potential risks due to HAPsemissions from facilities involved in coking operations.

Determination of Pesticide Residues in Cannabis Smoke

PubMed Central

Sullivan, Nicholas; Elzinga, Sytze; Raber, Jeffrey C.

2013-01-01

The present study was conducted in order to quantify to what extent cannabis consumers may be exposed to pesticide and other chemical residues through inhaled mainstream cannabis smoke. Three different smoking devices were evaluated in order to provide a generalized data set representative of pesticide exposures possible for medical cannabis users. Three different pesticides, bifenthrin, diazinon, and permethrin, along with the plant growth regulator paclobutrazol, which are readily available to cultivators in commercial products, were investigated in the experiment. Smoke generated from the smoking devices was condensed in tandem chilled gas traps and analyzed with gas chromatography-mass spectrometry (GC-MS). Recoveries of residues were as high as 69.5% depending on the device used and the component investigated, suggesting that the potential of pesticide and chemical residue exposures to cannabis users is substantial and may pose a significant toxicological threat in the absence of adequate regulatory frameworks. PMID:23737769

BENCHMARK ACCELERATED AGING OF HARVESTED HYPALON/EPR AND CSPE/XLPE POWER AND I&C CABLE IN NUCLEAR POWER PLANTS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Duckworth, Robert C; Fifield, Dr Leonard S

As part of the Light Water Reactor and Sustainability (LWRS) program in the U.S. Department of Energy (DOE) Office of Nuclear Energy, material aging and degradation research is currently geared to support the long-term operation of existing nuclear power plants (NPPs) as they move beyond their initial 40 year licenses. The goal of this research is to provide information so that NPPs can develop aging management programs (AMPs) to address replacement and monitoring needs as they look to operate for 20 years, and in some cases 40 years, beyond their initial operating lifetimes. For cable insulation and jacket materials thatmore » support instrument, control, and safety systems, accelerated aging data are needed to determine priorities in cable aging management programs. Before accelerated thermal and radiation aging of harvested, representative cable insulation and jacket materials, the benchmark performance of a new test capability at Oak Ridge National Laboratory (ORNL) was evaluated for temperatures between 70 and 135 C, dose rates between 100 and 500 Gy/h, and accumulated doses up to 20 kGy, Samples that were characterized and are representative of current materials in use were harvested from the Callaway NPP near Fulton, Missouri, and the San Onofre NPP north of San Diego, California. From the Callaway NPP, a multiconductor control rod cable manufactured by Boston Insulated Wire (BIW), with a Hypalon/ chorolosulfonated polyethylene (CSPE) jacket and ethylene-propylene rubber (EPR) insulation, was harvested from the auxiliary space during a planned outage in 2013. This cable was placed into service when the plant was started in 1984. From the San Onofre NPP, a Rockbestos Firewall III (FRIII) cable with a Hypalon/ CSPE jacket with cross-linked polyethylene (XLPE) insulation was harvested from an on-site, climate-controlled storage area. This conductor, which was never placed into service, was procured around 2007 in anticipation of future operation that did not

Redefining Agricultural Residues as Bioenergy Feedstocks

PubMed Central

Caicedo, Marlon; Barros, Jaime; Ordás, Bernardo

2016-01-01

The use of plant biomass is a sustainable alternative to the reduction of CO2 emissions. Agricultural residues are interesting bioenergy feedstocks because they do not compete with food and add extra value to the crop, which might help to manage these residues in many regions. Breeding crops for dual production of food and bioenergy has been reported previously, but the ideal plant features are different when lignocellulosic residues are burnt for heat or electricity, or fermented for biofuel production. Stover moisture is one of the most important traits in the management of agricultural waste for bioenergy production which can be modified by genetic improvement. A delayed leaf senescence or the stay-green characteristic contributes to higher grain and biomass yield in standard, low nutrient, and drought-prone environments. In addition, the stay-green trait could be favorable for the development of dual purpose varieties because this trait could be associated with a reduction in biomass losses and lodging. On the other hand, the stay-green trait could be detrimental for the management of agricultural waste if it is associated with higher stover moisture at harvest, although this hypothesis has been insufficiently tested. In this paper, a review of traits relevant to the development of dual purpose varieties is presented with particular emphasis on stover moisture and stay-green, because less attention has been paid to these important traits in the literature. The possibility of developing new varieties for combined production is discussed from a breeding perspective. PMID:28773750

[Influence of arbuscular mycorrhizal inoculation on growth and phoxim residue of carrot (Daucus carota L.)].

PubMed

Wang, Fa-Yuan; Chen, Xin; Sun, Xian-Ming; Shi, Zhao-Yong

2010-12-01

A pot culture experiment was carried out to study the influence of arbuscular mycorrhizal (AM) fungi on the growth and phoxim residue of carrot (Daucus carota L). Four levels of phoxim (0, 200, 400, 800 mg x L(-1)) and two AM fungal inocula, Glomus intraradices BEG 141(141), Glomus mosseae BEG 167 (167),and one nonmycorrhizal inoculum (CK), were applied to the sterilized soil. The plants were harvested after 5 months of growth and phoxim was irrigated into the root zone 14 d before plant harvest. Although decreasing with the increase of phoxim dosage, root infection rates of all the mycorrhizal plants were higher than 70%. Phoxim showed no significant dose effect on shoot wet weights and root yields, which were all increased by AM inoculation at four phoxim dosages. Phoxim residues in shoots and roots increased with the increase of phoxim dosage, but decreased by AM inoculation. In general, Glomus intraradices BEG 141 showed more pronounced effects on the growth and phoxim residue of carrot than Glomus mosseae BEG 167 did. Our results show a promising potential of AM fungi in carrot production and controlling pesticide residues.

Carbon and macronutrient loss during accelerated erosion under different tillage and residue management systems

USDA-ARS?s Scientific Manuscript database

The effects of tillage and crop residue removal on erosion and associated macronutrient fluxes on erodible soils subjected to a high intensity simulated rain event (70 mm/h) were investigated in an experimental watershed in Ohio, USA. A set of plots which constitute two experiments at this site were...

Accelerator-driven transmutation of spent fuel elements

DOEpatents

Venneri, Francesco; Williamson, Mark A.; Li, Ning

2002-01-01

An apparatus and method is described for transmuting higher actinides, plutonium and selected fission products in a liquid-fuel subcritical assembly. Uranium may also be enriched, thereby providing new fuel for use in conventional nuclear power plants. An accelerator provides the additional neutrons required to perform the processes. The size of the accelerator needed to complete fuel cycle closure depends on the neutron efficiency of the supported reactors and on the neutron spectrum of the actinide transmutation apparatus. Treatment of spent fuel from light water reactors (LWRs) using uranium-based fuel will require the largest accelerator power, whereas neutron-efficient high temperature gas reactors (HTGRs) or CANDU reactors will require the smallest accelerator power, especially if thorium is introduced into the newly generated fuel according to the teachings of the present invention. Fast spectrum actinide transmutation apparatus (based on liquid-metal fuel) will take full advantage of the accelerator-produced source neutrons and provide maximum utilization of the actinide-generated fission neutrons. However, near-thermal transmutation apparatus will require lower standing

Cell Wall Assembly and Intracellular Trafficking in Plant Cells Are Directly Affected by Changes in the Magnitude of Gravitational Acceleration

PubMed Central

Chebli, Youssef; Pujol, Lauranne; Shojaeifard, Anahid; Brouwer, Iman; van Loon, Jack J. W. A.; Geitmann, Anja

2013-01-01

Plants are able to sense the magnitude and direction of gravity. This capacity is thought to reside in selected cell types within the plant body that are equipped with specialized organelles called statoliths. However, most plant cells do not possess statoliths, yet they respond to changes in gravitational acceleration. To understand the effect of gravity on the metabolism and cellular functioning of non-specialized plant cells, we investigated a rapidly growing plant cell devoid of known statoliths and without gravitropic behavior, the pollen tube. The effects of hyper-gravity and omnidirectional exposure to gravity on intracellular trafficking and on cell wall assembly were assessed in Camellia pollen tubes, a model system with highly reproducible growth behavior in vitro. Using an epi-fluorescence microscope mounted on the Large Diameter Centrifuge at the European Space Agency, we were able to demonstrate that vesicular trafficking is reduced under hyper-gravity conditions. Immuno-cytochemistry confirmed that both in hyper and omnidirectional gravity conditions, the characteristic spatial profiles of cellulose and callose distribution in the pollen tube wall were altered, in accordance with a dose-dependent effect on pollen tube diameter. Our findings suggest that in response to gravity induced stress, the pollen tube responds by modifying cell wall assembly to compensate for the altered mechanical load. The effect was reversible within few minutes demonstrating that the pollen tube is able to quickly adapt to changing stress conditions. PMID:23516452

A technique for accelerating the convergence of restarted GMRES

DOE Office of Scientific and Technical Information (OSTI.GOV)

Baker, A H; Jessup, E R; Manteuffel, T

2004-03-09

We have observed that the residual vectors at the end of each restart cycle of restarted GMRES often alternate direction in a cyclic fashion, thereby slowing convergence. We present a new technique for accelerating the convergence of restarted GMRES by disrupting this alternating pattern. The new algorithm resembles a full conjugate gradient method with polynomial preconditioning, and its implementation requires minimal changes to the standard restarted GMRES algorithm.

Multiple beam induction accelerators for heavy ion fusion

NASA Astrophysics Data System (ADS)

Seidl, Peter A.; Barnard, John J.; Faltens, Andris; Friedman, Alex; Waldron, William L.

2014-01-01

Induction accelerators are appealing for heavy-ion driven inertial fusion energy (HIF) because of their high efficiency and their demonstrated capability to accelerate high beam current (≥10 kA in some applications). For the HIF application, accomplishments and challenges are summarized. HIF research and development has demonstrated the production of single ion beams with the required emittance, current, and energy suitable for injection into an induction linear accelerator. Driver scale beams have been transported in quadrupole channels of the order of 10% of the number of quadrupoles of a driver. We review the design and operation of induction accelerators and the relevant aspects of their use as drivers for HIF. We describe intermediate research steps that would provide the basis for a heavy-ion research facility capable of heating matter to fusion relevant temperatures and densities, and also to test and demonstrate an accelerator architecture that scales well to a fusion power plant.

Effects of prolonged acceleration with or without clinostat rotation on seedlings of Arabidopsis thaliana (L.) Heynh

NASA Technical Reports Server (NTRS)

Brown, A. H.; Dahl, A. O.; Loercher, L.

1974-01-01

Three 21-day tests of the effects of chronic centrifugation were carried out on populations of Arabidopsis thaliana. In addition to 1 g the resultant g-forces tested were: 2,4,6,8,16, and 20 g. Observed end points included gross morphological characters such as size of plant organs and, at the other extreme, features of sub-cellular structure and ultrastructure. Plants were grown on banks of clinostats. The acceleration vector was directed either parallel with the plants' axes or transverse to the axes. Plant responses to chronic axial acceleration and to transverse acceleration with clinostated plants were determined. From the data obtained it was possible in some cases: (1) to determine the g-functions of specific plant developmental characters; (2) to extrapolate those functions to the hypothetical value at zero g in order to predict (tentatively) the morphology of a plant grown in space, (3) to describe morphological effects of clinostat rotation, (4) to determine which of those effects was influenced by the prevailing g-force, and (5) to put to direct test the assumption that clinostat rotation nullifies or compensates for the influence of gravity.

Mutation of charged residues to neutral ones accelerates urea denaturation of HP-35.

PubMed

Wei, Haiyan; Yang, Lijiang; Gao, Yi Qin

2010-09-16

Following the studies of urea denaturation of β-hairpins using molecular dynamics, in this paper, molecular dynamics simulations of two peptides, a 35 residue three helix bundle villin headpiece protein HP-35 and its doubly norleucine-substituent mutant (Lys24Nle/Lys29Nle) HP-35 NleNle, were undertaken in urea solutions to understand the molecular mechanism of urea denaturation of α-helices. The mutant HP-35 NleNle was found to denature more easily than the wild type. During the expansion of the small hydrophobic core, water penetration occurs first, followed by that of urea molecules. It was also found that the initial hydration of the peptide backbone is achieved through water hydrogen bonding with the backbone CO groups during the denaturation of both polypeptides. The mutation of the two charged lysine residues to apolar norleucine enhances the accumulation of urea near the hydrophobic core and facilitates the denaturation process. Urea also interacts directly with the peptide backbone as well as side chains, thereby stabilizing nonnative conformations. The mechanism revealed here is consistent with the previous study on secondary structure of β-hairpin polypeptide, GB1, PEPTIDE 1, and TRPZIP4, suggesting that there is a general mechanism in the denaturation of protein backbone hydrogen bonds by urea.

Reburning Characteristics of Residual Carbon in Fly Ash from CFB Boilers

NASA Astrophysics Data System (ADS)

Zhang, S. H.; Luo, H. H.; Chen, H. P.; Yang, H. P.; Wang, X. H.

The content of residual carbon in fly ash of CFB boilers is a litter high especially when low-grade coal, such as lean coal, anthracite coal, gangue, etc. is in service, which greatly influences the efficiency of boilers and fly ash further disposal. Reburn of fly ash through collection, recirculation in CFB furnace or external combustor is a possibly effective strategy to decrease the carbon content, mainly depending on the residual carbon reactivity. In this work, the combustion properties of residual carbon in fly ash and corresponding original coal from large commercial CFB boilers (Kaifeng (440t/h), and Fenyi (410t/h), all in china) are comparably investigated through experiments. The residual carbon involved was firstly extracted and enriched from fly ash by means of floating elutriation to mitigate the influence of ash and minerals on the combustion behavior of residual carbon. Then, the combustion characteristic of two residual carbons and the original coal particles was analyzed with thermogravimetric analyzer (TGA, STA409C from Nestch, Germany). It was observed that the ignition temperature of the residual carbon is much higher than that of original coal sample, and the combustion reactivity of residual carbon is not only dependent on the original coal property, but also the operating conditions. The influence of oxygen content and heating rate was also studied in TGA. The O2 concentration is set as 20%, 30%, 40% and 70% respectively in O2/N2 gas mixture with the flow rate of 100ml/min. It was found that higher oxygen content is favor for decreasing ignition temperature, accelerating the combustion rate of residual carbon. And about 40% of oxygen concentration is experimentally suggested as an optimal value when oxygen-enriched combustion is put into practice for decreasing residual carbon content of fly ash in CFB boilers.

Say it with flowers: flowering acceleration by root communication.

PubMed

Falik, Omer; Hoffmann, Ishay; Novoplansky, Ariel

2014-01-01

The timing of reproduction is a critical determinant of fitness, especially in organisms inhabiting seasonal environments. Increasing evidence suggests that inter-plant communication plays important roles in plant functioning. Here, we tested the hypothesis that flowering coordination can involve communication between neighboring plants. We show that soil leachates from Brassica rapa plants growing under long-day conditions accelerated flowering and decreased allocation to vegetative organs in target plants growing under non-inductive short-day conditions. The results suggest that besides endogenous signaling and external abiotic cues, flowering timing may involve inter-plant communication, mediated by root exudates. The study of flowering communication is expected to illuminate neglected aspects of plant reproductive interactions and to provide novel opportunities for controlling the timing of plant reproduction in agricultural settings.

Say it with flowers: Flowering acceleration by root communication.

PubMed

Falik, Omer; Hoffmann, Ishay; Novoplansky, Ariel

2014-01-01

The timing of reproduction is a critical determinant of fitness, especially in organisms inhabiting seasonal environments. Increasing evidence suggests that inter-plant communication plays important roles in plant functioning. Here, we tested the hypothesis that flowering coordination can involve communication between neighboring plants. We show that soil leachates from Brassica rapa plants growing under long-day conditions accelerated flowering and decreased allocation to vegetative organs in target plants growing under non-inductive short-day conditions. The results suggest that besides endogenous signaling and external abiotic cues, flowering timing may involve inter-plant communication, mediated by root exudates. The study of flowering communication is expected to illuminate neglected aspects of plant reproductive interactions and to provide novel opportunities for controlling the timing of plant reproduction in agricultural settings.

Preliminary screening of ethnomedicinal plants from India.

PubMed

Perumal Samy, R; Ignacimuthu, S; Raja, D P

1999-08-01

Antibacterial activity of aqueous residues of 16 different ethnomedicinal plants have been studied. The effect of the aqueous extract at two different weights of plant residues, 30 and 40 mg, were tested against three gram positive bacteria and seven gram negative bacteria by the filter paper disc diffusion method. Among the tested plants, Cleome gynandropsis and Ageratum conyzoides showed a significant control of the growth of Alkaligens viscolactis, Klebsiella aerogenas, Bacillus cerues and Streptococcus pyogens. The maximum inhibitions were observed in Tridax procumbens, Cleome viscosa, Acalypha indica and Boerhaavia erecta against Aeromonas hydrophilla and Bacillus cerues.

Gametic embryogenesis and haploid technology as valuable support to plant breeding.

PubMed

Germanà, Maria Antonietta

2011-05-01

Plant breeding is focused on continuously increasing crop production to meet the needs of an ever-growing world population, improving food quality to ensure a long and healthy life and address the problems of global warming and environment pollution, together with the challenges of developing novel sources of biofuels. The breeders' search for novel genetic combinations, with which to select plants with improved traits to satisfy both farmers and consumers, is endless. About half of the dramatic increase in crop yield obtained in the second half of the last century has been achieved thanks to the results of genetic improvement, while the residual advance has been due to the enhanced management techniques (pest and disease control, fertilization, and irrigation). Biotechnologies provide powerful tools for plant breeding, and among these ones, tissue culture, particularly haploid and doubled haploid technology, can effectively help to select superior plants. In fact, haploids (Hs), which are plants with gametophytic chromosome number, and doubled haploids (DHs), which are haploids that have undergone chromosome duplication, represent a particularly attractive biotechnological method to accelerate plant breeding. Currently, haploid technology, making possible through gametic embryogenesis the single-step development of complete homozygous lines from heterozygous parents, has already had a huge impact on agricultural systems of many agronomically important crops, representing an integral part in their improvement programmes. The aim of this review was to provide some background, recent advances, and future prospective on the employment of haploid technology through gametic embryogenesis as a powerful tool to support plant breeding.

Debris and meteoroid proportions deduced from impact crater residue analysis

NASA Technical Reports Server (NTRS)

Berthoud, Lucinda; Mandeville, Jean-Claude; Durin, Christian; Borg, Janet

1995-01-01

This study is a further investigation of space-exposed samples recovered from the LDEF satellite and the Franco-Russian 'Aragatz' dust collection experiment on the Mir Space Station. Impact craters with diameters ranging from 1 to 900 micron were found on the retrieved samples. Elemental analysis of residues found in the impact craters was carried out using Energy Dispersive X-ray spectrometry (EDX). The analyses show evidence of micrometeoroid and orbital debris origins for the impacts. The proportions of these two components vary according to particle size and experimental position with respect to the leading edge of the spacecraft. On the LDEF leading edge 17 percent of the impacts were apparently caused by micrometeoroids and 11 percent by debris; on the LDEF trailing edge 23 percent of the impacts are apparently caused by micrometeoroids and 4 percent consist of debris particles - mostly larger than 3 micron in diameter - in elliptical orbits around the Earth. For Mir, the analyses indicate that micrometeoroids form 23 percent of impacts and debris 9 percent. However, we note that 60-70 percent of the craters are unidentifiable, so the definitive proportions of natural v. man-made particles are yet to be determined. Experiments carried out using a light gas gun to accelerate glass spheres and fragments demonstrate the influence of particle shape on crater morphology. The experiments also show that it is more difficult to analyze the residues produced by an irregular fragment than those produced by a spherical projectile. If the particle is travelling above a certain velocity, it vaporizes upon impact and no residues are left. Simulation experiments carried out with an electrostatic accelerator indicate that this limit is about 14 km/s for Fe particles impacting Al targets. This chemical analysis cut-off may bias interpretations of the relative populations of meteoroid and orbital debris. Oblique impacts and multiple foil detectors provide a higher likelihood

Evaluation of slice accelerations using multiband echo planar imaging at 3 Tesla

PubMed Central

Xu, Junqian; Moeller, Steen; Auerbach, Edward J.; Strupp, John; Smith, Stephen M.; Feinberg, David A.; Yacoub, Essa; Uğurbil, Kâmil

2013-01-01

We evaluate residual aliasing among simultaneously excited and acquired slices in slice accelerated multiband (MB) echo planar imaging (EPI). No in-plane accelerations were used in order to maximize and evaluate achievable slice acceleration factors at 3 Tesla. We propose a novel leakage (L-) factor to quantify the effects of signal leakage between simultaneously acquired slices. With a standard 32-channel receiver coil at 3 Tesla, we demonstrate that slice acceleration factors of up to eight (MB = 8) with blipped controlled aliasing in parallel imaging (CAIPI), in the absence of in-plane accelerations, can be used routinely with acceptable image quality and integrity for whole brain imaging. Spectral analyses of single-shot fMRI time series demonstrate that temporal fluctuations due to both neuronal and physiological sources were distinguishable and comparable up to slice-acceleration factors of nine (MB = 9). The increased temporal efficiency could be employed to achieve, within a given acquisition period, higher spatial resolution, increased fMRI statistical power, multiple TEs, faster sampling of temporal events in a resting state fMRI time series, increased sampling of q-space in diffusion imaging, or more quiet time during a scan. PMID:23899722

Combined laser in-situ keratomileusis and accelerated corneal cross-linking: an update.

PubMed

Tomita, Minoru

2016-07-01

The purpose is to review the literature of combined laser in-situ keratomileusis (LASIK) and accelerated corneal collagen cross-linking (CXL) in context of its indications-contraindications, kerato-refractive, visual and safety outcomes, particularly with reference to preventing the development of post-LASIK ectasia. LASIK + accelerated CXL has been developed with the rationale that the addition of CXL after LASIK may strengthen the LASIK compromised corneal biomechanics and minimize the complications such as post-LASIK ectasia. Different clinical studies have documented the safety and efficacy of LASIK + accelerated CXL for the correction of myopia or hyperopia and in the patients with low predicted residual bed thickness. Available literature shows that refractive and keratometric outcomes of LASIK + accelerated CXL are comparable or better than LASIK alone. Less regression has been observed after LASIK + accelerated CXL compared with LASIK alone and no case of post-LASIK ectasia development has been reported among 673 eyes with the follow-up ranging from 3 months to 4.5 years. Future studies with large numbers of patients and longer postoperative follow-ups are needed to establish the efficacy of LASIK + accelerated CXL in preventing the development of post-LASIK ectasia.

Residual Stress Measurement and the Effect of Heat Treatment in Cladded Control Rod Drive Specimens

NASA Astrophysics Data System (ADS)

Bowman, Ashley; Kingston, Ed; Katsuyama, Jinya; Udagawa, Makoto; Onizawa, Kunio

This paper presents results of residual stress measurements and modelling within the cladding and J-groove weld of Control Rod Drive (CRD) specimens in the as-welded and Post Weld Heat Treated (PWHT) states. Knowledge of the residual stresses present in CRD nozzles is critical when modelling the fracture mechanics of failures of nuclear power plant components to dictate inspections intervals and optimise plant downtime. The specimens comprised of ferritic steel blocks with 309L stainless steel cladding and a single J-groove weld attaching the 304 stainless steel nozzles. Multiple measurements were made through the thickness of the specimens in order to give biaxial residual stress profiles through all the different fusion boundaries. The results show the effect of PWHT in reducing residual stresses both in the weld and ferritic material. The beneficial use of measurements is highlighted to provide confidence in the modelled results and prevent over conservatism in integrity calculations, costing unnecessary time and money.

How do soil quality indicators (SOC and nutrients) change with long-term different crop residue management?

NASA Astrophysics Data System (ADS)

Spiegel, Heide; Lehtinen, Taru; Dersch, Georg; Baumgarten, Andreas

2016-04-01

Leaving the crop residues (cereal grain straw, maize stover, sugar beet leaves) on the field may enhance SOC and soil nutrient contents (e.g. P, K, Mg). In contrast, harvesting crop residues for livestock bedding or energy production are often connected with a loss of soil fertility (Lehtinen et al., 2014). We have evaluated the effects of different management of crop residues on selected soil parameters of the upper soil (0-25 cm) in two long-term field experiments in Austria focused on P-dynamics (Marchfeld, since 1982 and Alpenvorland, since 1986). In four P-fertilisation stages (0, 75, 150, 300 kg P2O5 ha-1y-1) all crop residues were incorporated in one treatment and all removed in the other one, respectively. The results show that the effects are different at the two investigated sites. At the site Marchfeld, a medium textured soil, on average SOC was significantly higher with the incorporation of crop residues (21.6 g kg-1) compared to the removal (19.9 g kg-1) after 32 years. In the long run, SOC levels could be maintained, if crop residues remained at the field, whereas the constant removal of crop residues resulted in a SOC decline. At the site Alpenvorland, SOC was only slightly higher with the incorporation of the crop residues after 28 years. In this case, in the long run, even with this management practice and, moreover, with the residue removal, SOC tended to decrease generally. At the Marchfeld, crop residue incorporation resulted in a significant increase of "plant available" phosphorus (P-CAL) only with very high P fertilization. However, "plant available" Mg (according to Schachtschabel) and potassium (K-CAL) were significantly higher in all P fertilisation stages compared to the residue removal treatments. At the site Alpenvorland, the soils are rich in silt and clay and with long-term incorporation of crop residues a significant increase only of „plant available" K of about 50% occurred. This indicates the necessity of taking into account the

Residual transpiration as a component of salinity stress tolerance mechanism: a case study for barley.

PubMed

Hasanuzzaman, Md; Davies, Noel W; Shabala, Lana; Zhou, Meixue; Brodribb, Tim J; Shabala, Sergey

2017-06-19

While most water loss from leaf surfaces occurs via stomata, part of this loss also occurs through the leaf cuticle, even when the stomata are fully closed. This component, termed residual transpiration, dominates during the night and also becomes critical under stress conditions such as drought or salinity. Reducing residual transpiration might therefore be a potentially useful mechanism for improving plant performance when water availability is reduced (e.g. under saline or drought stress conditions). One way of reducing residual transpiration may be via increased accumulation of waxes on the surface of leaf. Residual transpiration and wax constituents may vary with leaf age and position as well as between genotypes. This study used barley genotypes contrasting in salinity stress tolerance to evaluate the contribution of residual transpiration to the overall salt tolerance, and also investigated what role cuticular waxes play in this process. Leaves of three different positions (old, intermediate and young) were used. Our results show that residual transpiration was higher in old leaves than the young flag leaves, correlated negatively with the osmolality, and was positively associated with the osmotic and leaf water potentials. Salt tolerant varieties transpired more water than the sensitive variety under normal growth conditions. Cuticular waxes on barley leaves were dominated by primary alcohols (84.7-86.9%) and also included aldehydes (8.90-10.1%), n-alkanes (1.31-1.77%), benzoate esters (0.44-0.52%), phytol related compounds (0.22-0.53%), fatty acid methyl esters (0.14-0.33%), β-diketones (0.07-0.23%) and alkylresorcinols (1.65-3.58%). A significant negative correlation was found between residual transpiration and total wax content, and residual transpiration correlated significantly with the amount of primary alcohols. Both leaf osmolality and the amount of total cuticular wax are involved in controlling cuticular water loss from barley leaves under well

Onsager's symmetry relation and the residual parallel Reynolds stress in a magnetized plasma with electrostatic turbulence

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zuo, Yang, E-mail: yangzustc@gmail.com; Wang, Shaojie

2014-09-15

The physics of the residual parallel Reynolds stress in a rotating plasma with electrostatic turbulence is explicitly identified by using the transport formulation of the gyrokinetic turbulence. It is clarified that the residual stress consists of four terms, among which are the cross terms due to the pressure gradient and the temperature gradient and the terms related to the turbulent acceleration impulse and the turbulent heating rate. The last two terms are identified for the first time, and are shown to cause analogous residual term in the heat flux. Meanwhile, the transport matrix reveals diffusion in the phase space. Themore » transport matrix is demonstrated to satisfy the Onsager's symmetry relation.« less

Recovering metals from sewage sludge, waste incineration residues and similar substances with hyperaccumulative plants

NASA Astrophysics Data System (ADS)

Kisser, Johannes; Gattringer, Heinz; Iordanopoulos-Kisser, Monika

2015-04-01

observed in so-called hyperaccumulating metalophytes, which are studied for its suitability to be incorporated in metal recovery processes of elements that diffusely occur in different waste streams. In a systematic series of tests under laboratory conditions the accumulation behaviour for many different elements including rare earth metals of a selection of candidate plants growing on sewage sludge, incineration residues and industrial leftovers was assessed (quantitavely and qualitatively). Growth performance of these plants as well as the most suitable substrate properties were evaluated. The results of this project provided the groundwork for further research and development steps that might bring to practical implementation a technological option with potentially huge benefits: The recovery of valuable metal resources from sewage sludge, incineration ashes and metal rich wastewaters by environmentally friendly and low energy means. Simultaneous decontamination of the input substrates from heavy metals, opening the possibility for these nutrient streams to be redirected to biological regeneration processes (for example use as fertilizers in agriculture) without fear of polluting soils with heavy metal loads. Generation of biomass on contaminated substrates can yield usable energy surplus through incineration during or after processing.

Benchmark Accelerated Aging of Harvested Hypalon/Epr and Cspe/Xlpe Power and I&C Cable in Nuclear Power Plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Duckworth, Robert C.; Frame, Emily; Fifield, Leonard S.

As part of the Light Water Reactor and Sustainability (LWRS) program in the U.S. Department of Energy (DOE) Office of Nuclear Energy, material aging and degradation research is currently geared to support the long-term operation of existing nuclear power plants (NPPs) as they move beyond their initial 40 year licenses. The goal of this research is to provide information so that NPPs can develop aging management programs (AMPs) to address replacement and monitoring needs as they look to operate for 20 years, and in some cases 40 years, beyond their initial operating lifetimes. For cable insulation and jacket materials thatmore » support instrument, control, and safety systems, accelerated aging data are needed to determine priorities in cable aging management programs. Before accelerated thermal and radiation aging of harvested, representative cable insulation and jacket materials, the benchmark performance of a new test capability at Oak Ridge National Laboratory (ORNL) was evaluated for temperatures between 70 and 135°C, dose rates between 100 and 500 Gy/h, and accumulated doses up to 20 kGy, Samples that were characterized and are representative of current materials in use were harvested from the Callaway NPP near Fulton, Missouri, and the San Onofre NPP north of San Diego, California. From the Callaway NPP, a multiconductor control rod cable manufactured by Boston Insulated Wire (BIW), with a Hypalon/ chorolosulfonated polyethylene (CSPE) jacket and ethylene-propylene rubber (EPR) insulation, was harvested from the auxiliary space during a planned outage in 2013. This cable was placed into service when the plant was started in 1984. From the San Onofre NPP, a Rockbestos Firewall III (FRIII) cable with a Hypalon/ CSPE jacket with cross-linked polyethylene (XLPE) insulation was harvested from an on-site, climate-controlled storage area. This conductor, which was never placed into service, was procured around 2007 in anticipation of future operation that did not

On tide-induced Lagrangian residual current and residual transport: 1. Lagrangian residual current

USGS Publications Warehouse

Feng, Shizuo; Cheng, Ralph T.; Pangen, Xi

1986-01-01

Residual currents in tidal estuaries and coastal embayments have been recognized as fundamental factors which affect the long-term transport processes. It has been pointed out by previous studies that it is more relevant to use a Lagrangian mean velocity than an Eulerian mean velocity to determine the movements of water masses. Under weakly nonlinear approximation, the parameter k, which is the ratio of the net displacement of a labeled water mass in one tidal cycle to the tidal excursion, is assumed to be small. Solutions for tides, tidal current, and residual current have been considered for two-dimensional, barotropic estuaries and coastal seas. Particular attention has been paid to the distinction between the Lagrangian and Eulerian residual currents. When k is small, the first-order Lagrangian residual is shown to be the sum of the Eulerian residual current and the Stokes drift. The Lagrangian residual drift velocity or the second-order Lagrangian residual current has been shown to be dependent on the phase of tidal current. The Lagrangian drift velocity is induced by nonlinear interactions between tides, tidal currents, and the first-order residual currents, and it takes the form of an ellipse on a hodograph plane. Several examples are given to further demonstrate the unique properties of the Lagrangian residual current.

Finite element residual stress analysis of induction heating bended ferritic steel piping

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kima, Jong Sung; Kim, Kyoung-Soo; Oh, Young-Jin

2014-10-06

Recently, there is a trend to apply the piping bended by induction heating process to nuclear power plants. Residual stress can be generated due to thermo-mechanical mechanism during the induction heating bending process. It is well-known that the residual stress has important effect on crack initiation and growth. The previous studies have focused on the thickness variation. In part, some studies were performed for residual stress evaluation of the austenitic stainless steel piping bended by induction heating. It is difficult to find the residual stresses of the ferritic steel piping bended by the induction heating. The study assessed the residualmore » stresses of induction heating bended ferriticsteel piping via finite element analysis. As a result, it was identified that high residual stresses are generated on local outersurface region of the induction heating bended ferritic piping.« less

Acceleration modules in linear induction accelerators

NASA Astrophysics Data System (ADS)

Wang, Shao-Heng; Deng, Jian-Jun

2014-05-01

The Linear Induction Accelerator (LIA) is a unique type of accelerator that is capable of accelerating kilo-Ampere charged particle current to tens of MeV energy. The present development of LIA in MHz bursting mode and the successful application into a synchrotron have broadened LIA's usage scope. Although the transformer model is widely used to explain the acceleration mechanism of LIAs, it is not appropriate to consider the induction electric field as the field which accelerates charged particles for many modern LIAs. We have examined the transition of the magnetic cores' functions during the LIA acceleration modules' evolution, distinguished transformer type and transmission line type LIA acceleration modules, and re-considered several related issues based on transmission line type LIA acceleration module. This clarified understanding should help in the further development and design of LIA acceleration modules.

Glyphosate decreases mycorrhizal colonization and affects plant-soil feedback.

PubMed

Helander, Marjo; Saloniemi, Irma; Omacini, Marina; Druille, Magdalena; Salminen, Juha-Pekka; Saikkonen, Kari

2018-06-11

Our aim was to study the effects of glyphosate, tilling practice and cultivation history on mycorrhizal colonization and growth of target (weeds) and non-target (crops) plants. Glyphosate, the world's most widely used pesticide, inhibits an enzyme found in plants but also in microbes. We examined the effects of glyphosate treatment applied in the preceding fall on growth of a perennial weed, Elymus repens (target plant) and a forage grass, Festuca pratensis (non-target plant) and their arbuscular mycorrhizal fungal (AMF) root colonization in a field pot experiment. Non-target plants were sown in the following spring. Furthermore, we tested if glyphosate effects depend on tillage or soil properties modulated by long cultivation history of endophyte symbiotic grass (E+ grass). AMF root colonization, plant establishment and growth, glyphosate residues in plants, and soil chemistry were measured. Glyphosate reduced the mycorrhizal colonization and growth of both target and non-target grasses. The magnitude of reduction depended on tillage and soil properties due to cultivation history of E+ grass. We detected glyphosate residues in weeds and crop plants in the growing season following the glyphosate treatment. Residues were higher in plants growing in no-till pots compared to conspecifics in tilled pots. These results demonstrate negative effects of glyphosate on non-target organisms in agricultural environments and grassland ecosystems. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Bio-oil production via fast pyrolysis of biomass residues from cassava plants in a fluidised-bed reactor.

PubMed

Pattiya, Adisak

2011-01-01

Biomass residues from cassava plants, namely cassava stalk and cassava rhizome, were pyrolysed in a fluidised-bed reactor for production of bio-oil. The aims of this work were to investigate the yields and properties of pyrolysis products produced from both feedstocks as well as to identify the optimum pyrolysis temperature for obtaining the highest organic bio-oil yields. Results showed that the maximum yields of the liquid bio-oils derived from the stalk and rhizome were 62 wt.% and 65 wt.% on dry basis, respectively. The pyrolysis temperatures that gave highest bio-oil yields for both feedstocks were in the range of 475-510 °C. According to the analysis of the bio-oils properties, the bio-oil derived from cassava rhizome showed better quality than that derived from cassava stalk as the former had lower oxygen content, higher heating value and better storage stability. Copyright © 2010 Elsevier Ltd. All rights reserved.

Retaining Residual Ovarian Tissue following Ovarian Failure Has Limited Influence on Bone Loss in Aged Mice

PubMed Central

Craig, Zelieann R.; Marion, Samuel L.; Funk, Janet L.; Bouxsein, Mary L.; Hoyer, Patricia B.

2010-01-01

Previous work showed that retaining residual ovarian tissue protects young mice from accelerated bone loss following ovarian failure. The present study was designed to determine whether this protection is also present in aged animals. Aged (9–12 months) C57BL/6Hsd female mice were divided into: CON (vehicle), VCD (160 mg/kg; 15d), or OVX (ovariectomized). Lumbar BMD was monitored by DXA and μCT used to assess vertebral microarchitecture. BMD was not different between VCD and CON at any time point but was lower (P < .05) than baseline, starting 1 month after ovarian failure in VCD and OVX mice. Following μCT analysis there were no differences between CON and VCD, but OVX mice had lower bone volume fraction, trabecular thickness, and a trend for decreased connectivity density. These findings provide evidence that retention of residual ovarian tissue may protect aged follicle-depleted mice from accelerated bone loss to a lesser extent than that observed in young mice. PMID:20948577

Quality and generation rate of solid residues in the boiler of a waste-to-energy plant.

PubMed

Allegrini, E; Boldrin, A; Jansson, S; Lundtorp, K; Fruergaard Astrup, T

2014-04-15

The Danish waste management system relies significantly on waste-to-energy (WtE) plants. The ash produced at the energy recovery section (boiler ash) is classified as hazardous waste, and is commonly mixed with fly ash and air pollution control residues before disposal. In this study, a detailed characterization of boiler ash from a Danish grate-based mass burn type WtE was performed, to evaluate the potential for improving ash management. Samples were collected at 10 different points along the boiler's convective part, and analysed for grain size distribution, content of inorganic elements, polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD and PCDF), and leaching of metals. For all samples, PCDD and PCDF levels were below regulatory limits, while high pH values and leaching of e.g. Cl were critical. No significant differences were found between boiler ash from individual sections of the boiler, in terms of total content and leaching, indicating that separate management of individual ash fractions may not provide significant benefits. Copyright © 2014 Elsevier B.V. All rights reserved.

Thorium, uranium and rare earth elements content in lanthanide concentrate (LC) and water leach purification (WLP) residue of Lynas advanced materials plant (LAMP)

DOE Office of Scientific and Technical Information (OSTI.GOV)

AL-Areqi, Wadeeah M., E-mail: walareqi@yahoo.com; Majid, Amran Ab., E-mail: walareqi@yahoo.com; Sarmani, Sukiman, E-mail: walareqi@yahoo.com

Lynas Advanced Materials Plant (LAMP) has been licensed to produce the rare earths elements since early 2013 in Malaysia. LAMP processes lanthanide concentrate (LC) to extract rare earth elements and subsequently produce large volumes of water leach purification (WLP) residue containing naturally occurring radioactive material (NORM). This residue has been rising up the environmental issue because it was suspected to accumulate thorium with significant activity concentration and has been classified as radioactive residue. The aim of this study is to determine Th-232, U-238 and rare earth elements in lanthanide concentrate (LC) and water leach purification (WLP) residue collected from LAMPmore » and to evaluate the potential radiological impacts of the WLP residue on the environment. Instrumental Neutron Activation Analysis and γ-spectrometry were used for determination of Th, U and rare earth elements concentrations. The results of this study found that the concentration of Th in LC was 1289.7 ± 129 ppm (5274.9 ± 527.6Bq/kg) whereas the Th and U concentrations in WLP were determined to be 1952.9±17.6 ppm (7987.4 ± 71.9 Bq/kg) and 17.2 ± 2.4 ppm respectively. The concentrations of Th and U in LC and WLP samples determined by γ- spectrometry were 1156 ppm (4728 ± 22 Bq/kg) and 18.8 ppm and 1763.2 ppm (7211.4 Bq/kg) and 29.97 ppm respectively. This study showed that thorium concentrations were higher in WLP compare to LC. This study also indicate that WLP residue has high radioactivity of {sup 232}Th compared to Malaysian soil natural background (63 - 110 Bq/kg) and come under preview of Act 304 and regulations. In LC, the Ce and Nd concentrations determined by INAA were 13.2 ± 0.6% and 4.7 ± 0.1% respectively whereas the concentrations of La, Ce, Nd and Sm in WLP were 0.36 ± 0.04%, 1.6%, 0.22% and 0.06% respectively. This result showed that some amount of rare earth had not been extracted and remained in the WLP and may be considered to be reextracted.« less

Thorium, uranium and rare earth elements content in lanthanide concentrate (LC) and water leach purification (WLP) residue of Lynas advanced materials plant (LAMP)

NASA Astrophysics Data System (ADS)

AL-Areqi, Wadeeah M.; Majid, Amran Ab.; Sarmani, Sukiman

2014-02-01

Lynas Advanced Materials Plant (LAMP) has been licensed to produce the rare earths elements since early 2013 in Malaysia. LAMP processes lanthanide concentrate (LC) to extract rare earth elements and subsequently produce large volumes of water leach purification (WLP) residue containing naturally occurring radioactive material (NORM). This residue has been rising up the environmental issue because it was suspected to accumulate thorium with significant activity concentration and has been classified as radioactive residue. The aim of this study is to determine Th-232, U-238 and rare earth elements in lanthanide concentrate (LC) and water leach purification (WLP) residue collected from LAMP and to evaluate the potential radiological impacts of the WLP residue on the environment. Instrumental Neutron Activation Analysis and γ-spectrometry were used for determination of Th, U and rare earth elements concentrations. The results of this study found that the concentration of Th in LC was 1289.7 ± 129 ppm (5274.9 ± 527.6Bq/kg) whereas the Th and U concentrations in WLP were determined to be 1952.9±17.6 ppm (7987.4 ± 71.9 Bq/kg) and 17.2 ± 2.4 ppm respectively. The concentrations of Th and U in LC and WLP samples determined by γ- spectrometry were 1156 ppm (4728 ± 22 Bq/kg) & 18.8 ppm and 1763.2 ppm (7211.4 Bq/kg) &29.97 ppm respectively. This study showed that thorium concentrations were higher in WLP compare to LC. This study also indicate that WLP residue has high radioactivity of 232Th compared to Malaysian soil natural background (63 - 110 Bq/kg) and come under preview of Act 304 and regulations. In LC, the Ce and Nd concentrations determined by INAA were 13.2 ± 0.6% and 4.7 ± 0.1% respectively whereas the concentrations of La, Ce, Nd and Sm in WLP were 0.36 ± 0.04%, 1.6%, 0.22% and 0.06% respectively. This result showed that some amount of rare earth had not been extracted and remained in the WLP and may be considered to be reextracted.

Accelerated DNA Methylation Age: Associations with PTSD and Neural Integrity

PubMed Central

Wolf, Erika J.; Logue, Mark W.; Hayes, Jasmeet P.; Sadeh, Naomi; Schichman, Steven A.; Stone, Annjanette; Salat, David H.; Milberg, William; McGlinchey, Regina; Miller, Mark W.

2015-01-01

Background Accumulating evidence suggests that post traumatic stress disorder (PTSD) may accelerate cellular aging and lead to premature morbidity and neurocognitive decline. Methods This study evaluated associations between PTSD and DNA methylation (DNAm) age using recently developed algorithms of cellular age by Horvath (2013) and Hannum et al. (2013). These estimates reflect accelerated aging when they exceed chronological age. We also examined if accelerated cellular age manifested in degraded neural integrity, indexed via diffusion tensor imaging. Results Among 281 male and female veterans of the conflicts in Iraq and Afghanistan, DNAm age was strongly related to chronological age (rs ~.88). Lifetime PTSD severity was associated with Hannum DNAm age estimates residualized for chronological age (β = .13, p= .032). Advanced DNAm age was associated with reduced integrity in the genu of the corpus callosum (β = −.17, p= .009) and indirectly linked to poorer working memory performance via this region (indirect β = − .05, p= .029). Horvath DNAm age estimates were not associated with PTSD or neural integrity. Conclusions Results provide novel support for PTSD-related accelerated aging in DNAm and extend the evidence base of known DNAm age correlates to the domains of neural integrity and cognition. PMID:26447678

Spatial and temporal distribution of 13C labelled plant residues in soil aggregates and Lumbricus terrestris surface casts: A combination of Transmission Electron Microscopy and Nanoscale Secondary Ion Mass Spectrometry

NASA Astrophysics Data System (ADS)

Vidal, Alix; Remusat, Laurent; Watteau, Françoise; Derenne, Sylvie; Quenea, Katell

2016-04-01

Earthworms play a central role in litter decomposition, soil structuration and carbon cycling. They ingest both organic and mineral compounds which are mixed, complexed with mucus and dejected in form of casts at the soil surface and along burrows. Bulk isotopic or biochemical technics have often been used to study the incorporation of litter in soil and casts, but they could not reflect the complex interaction between soil, plant and microorganisms at the microscale. However, the heterogeneous distribution of organic carbon in soil structures induces contrasted microbial activity areas. Nano-scale secondary ion mass spectrometry (NanoSIMS), which is a high spatial resolution method providing elemental and isotopic maps of organic and mineral materials, has recently been applied in soil science (Herrmann et al., 2007; Vogel et al., 2014). The combination of Nano-scale secondary ion mass spectrometry (NanoSIMS) and Transmission Electron Microscopy (TEM) has proven its potential to investigate labelled residues incorporation in earthworm casts (Vidal et al., 2016). In line of this work, we studied the spatial and temporal distribution of plant residues in soil aggregates and earthworm surface casts. This study aimed to (1) identify the decomposition states of labelled plant residues incorporated at different time steps, in casts and soil, (2) identify the microorganisms implied in this decomposition (3) relate the organic matter states of decomposition with their 13C signature. A one year mesocosm experiment was set up to follow the incorporation of 13C labelled Ryegrass (Lolium multiflorum) litter in a soil in the presence of anecic earthworms (Lumbricus terrestris). Soil and surface cast samples were collected after 8 and 54 weeks, embedded in epoxy resin and cut into ultra-thin sections. Soil was fractionated and all and analyzed with TEM and NanoSIMS, obtaining secondary ion images of 12C, 16O, 12C14N, 13C14N and 28Si. The δ13C maps were obtained using the 13C14

Impact of diverse soil microbial communities on crop residues decomposition

NASA Astrophysics Data System (ADS)

Mrad, Fida; Bennegadi-Laurent, Nadia; Ailhas, Jérôme; Leblanc, Nathalie; Trinsoutrot-Gattin, Isabelle; Laval, Karine; Gattin, Richard

2017-04-01

Soils provide many basic ecosystem services for our society and most of these services are carried out by the soil communities, thus influencing soils quality. Soil organic matter (SOM) can be considered as one of the most important soil quality indices for it plays a determinant role in many physical, chemical and biological processes, such as soil structure and erosion resistance, cation exchange capacity, nutrient cycling and biological activity (Andrews et al., 2004). Since a long time, exogenous organic inputs are largely used for improving agricultural soils, affecting highly soil fertility and productivity. The use of organic amendments such as crop residues influences the soil microbial populations' diversity and abundance. In the meantime, soil microbial communities play a major role in the organic matter degradation, and the effect of different microbial communities on the decomposition of crop residues is not well documented. In this context, studying the impact of crop residues on soil microbial ecology and the processes controlling the fate of plant residues in different management practices is essential for understanding the long-term environmental and agronomic effects on soil and organic matters. Our purpose in the present work was to investigate the decomposition by two contrasting microbial communities of three crop residues, and compare the effect of different residues amendments on the abundance and function of each soil microbial communities. Among the main crops which produce large amounts of residues, we focused on three different plants: wheat (Triticum aestivum L.), rape (Brassica napus) and sunflower (Helianthus annuus). The residues degradation in two soils of different management practices and the microbial activity were evaluated by: microbial abundance (microbial carbon, culturable bacteria, total DNA, qPCR), in combination with functional indicators (enzymatic assays and Biolog substrate utilization), kinetics of C and N

Performance of mechanical biological treatment of residual municipal waste in Poland

NASA Astrophysics Data System (ADS)

den Boer, Emilia; Jędrczak, Andrzej

2017-11-01

The number and capacity of mechanical-biological treatment (MBT) plants in Europe increased significantly in the past two decades as a response to the legal obligation to limit the landfilling of biodegradable waste in landfills and to increase recycling and energy recovery from waste. The aim of these plants is to prepare residual municipal waste for recovery and disposal operations, including especially separation and stabilization of the easily biodegradable fraction (the biofraction). The final products of MBP technology are recyclables, stabilate, high calorific fraction which is used for the production of refuse derived fuel (RDF) and the remaining residual fraction. The shares of the output fractions, especially of the recyclables and RDF determine the overall efficiency of MBT technology in diverting waste from landfills. In this paper results of an assessment of one exemplary MBT plant are provided. The analysis was performed within a comparative study in which 20 selected MBT plants in Poland were subject to a detailed analysis, focusing, both at the design parameters as well as operational ones. The selected plant showed relatively higher overall materials recovery efficiency. With the view to circular economy targets, increased automation of the mechanical waste treatment will be required to support achieving high level diversion from landfills. The study reviled that stabilisation of biofraction should be improved by a better control of process conditions, especially moisture content.

Optimisation of biocide dose as a function of residual biocide in a heat exchanger pilot plant effluent.

PubMed

Eguía, Emilio; Trueba, Alfredo; Girón, Alfredo; Río-Calonge, Belén; Otero, Félix; Bielva, Carlos

2007-01-01

Biofouling is one of the most serious problems facing numerous industrial processes. In the case of a heat exchanger unit, biological deposits adhering to the inside surface of its tubes reduce heat transfer and, thus, the thermal performance of the cycle. Control of this phenomenon is proving fundamental for both land and marine equipment to operate in optimum working conditions. Hence, it is necessary to apply antifouling methods capable of keeping surfaces free of any kind of biofouling. This paper reports on the behaviour resulting from use of the flow inversion method vs that obtained by using various chemical treatments. The study compares the effectiveness of certain chemical treatments (Na hypochlorite, peracetic acid and a compound formed by Na bromide + Na hypochlorite) for removing a biofouling film that has already formed on the inside surfaces of tubes in a heat exchanger pilot plant. The paper also addresses the issue of optimising the concentration of biocide dose as a function of the residual biocide in order minimise the environmental impact caused by effluent from industrial plants. The results indicate that it is possible to eliminate a biofilm formed on the inside surfaces of tubes by the use of intermittent doses of chemical treatments at low concentrations and over long application times. Furthermore, once the stabilisation phase is reached 6 d after starting the treatment, it is possible to maintain the conditions achieved using only 20% of the initial dosage.

Single event effects in high-energy accelerators

NASA Astrophysics Data System (ADS)

García Alía, Rubén; Brugger, Markus; Danzeca, Salvatore; Cerutti, Francesco; de Carvalho Saraiva, Joao Pedro; Denz, Reiner; Ferrari, Alfredo; Foro, Lionel L.; Peronnard, Paul; Røed, Ketil; Secondo, Raffaello; Steckert, Jens; Thurel, Yves; Toccafondo, Iacocpo; Uznanski, Slawosz

2017-03-01

The radiation environment encountered at high-energy hadron accelerators strongly differs from the environment relevant for space applications. The mixed-field expected at modern accelerators is composed of charged and neutral hadrons (protons, pions, kaons and neutrons), photons, electrons, positrons and muons, ranging from very low (thermal) energies up to the TeV range. This complex field, which is extensively simulated by Monte Carlo codes (e.g. FLUKA) is due to beam losses in the experimental areas, distributed along the machine (e.g. collimation points) and deriving from the interaction with the residual gas inside the beam pipe. The resulting intensity, energy distribution and proportion of the different particles largely depends on the distance and angle with respect to the interaction point as well as the amount of installed shielding material. Electronics operating in the vicinity of the accelerator will therefore be subject to both cumulative damage from radiation (total ionizing dose, displacement damage) as well as single event effects which can seriously compromise the operation of the machine. This, combined with the extensive use of commercial-off-the-shelf components due to budget, performance and availability reasons, results in the need to carefully characterize the response of the devices and systems to representative radiation conditions.

Vibration isolation technology: Sensitivity of selected classes of space experiments to residual accelerations

NASA Technical Reports Server (NTRS)

Alexander, J. Iwan D.; Zhang, Y. Q.; Adebiyi, Adebimpe

1989-01-01

Progress performed on each task is described. Order of magnitude analyses related to liquid zone sensitivity and thermo-capillary flow sensitivity are covered. Progress with numerical models of the sensitivity of isothermal liquid zones is described. Progress towards a numerical model of coupled buoyancy-driven and thermo-capillary convection experiments is also described. Interaction with NASA personnel is covered. Results to date are summarized and they are discussed in terms of the predicted space station acceleration environment. Work planned for the second year is also discussed.

Preliminary Results from Pyroelectric Crystal Accelerator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anderson, Tom; Edwards, Ronald; Bright, Kevin

The Nuclear Science and Engineering Research Center (NSERC), a Defense Threat Reduction Agency (DTRA) office located at the United States Military Academy (USMA), sponsors and manages cadet and faculty research in support of DTRA objectives. Cadets in the Department of Physics and Nuclear Engineering at USMA are using pyroelectric crystals to ionize and accelerate residual gas trapped inside a vacuum system. A system using two lithium tantalate crystals with associated diagnostics was designed and is now operational. X-ray energies of approximately 150 keV have been achieved. Future work will focus on developing a portable neutron generator using the D-D nuclearmore » fusion process.« less

Soil and water warming accelerates phenology and down-regulation of leaf photosynthesis of rice plants grown under free-air CO2 enrichment (FACE).

PubMed

Adachi, Minaco; Hasegawa, Toshihiro; Fukayama, Hiroshi; Tokida, Takeshi; Sakai, Hidemitsu; Matsunami, Toshinori; Nakamura, Hirofumi; Sameshima, Ryoji; Okada, Masumi

2014-02-01

To enable prediction of future rice production in a changing climate, we need to understand the interactive effects of temperature and elevated [CO2] (E[CO2]). We therefore examined if the effect of E[CO2] on the light-saturated leaf photosynthetic rate (Asat) was affected by soil and water temperature (NT, normal; ET, elevated) under open-field conditions at the rice free-air CO2 enrichment (FACE) facility in Shizukuishi, Japan, in 2007 and 2008. Season-long E[CO2] (+200 µmol mol(-1)) increased Asat by 26%, when averaged over two years, temperature regimes and growth stages. The effect of ET (+2°C) on Asat was not significant at active tillering and heading, but became negative and significant at mid-grain filling; Asat in E[CO2]-ET was higher than in ambient [CO2] (A[CO2])-NT by only 4%. Photosynthetic down-regulation at E[CO2] also became apparent at mid-grain filling; Asat compared at the same [CO2] in the leaf cuvette was significantly lower in plants grown in E[CO2] than in those grown in A[CO2]. The additive effects of E[CO2] and ET decreased Asat by 23% compared with that of A[CO2]-NT plants. Although total crop nitrogen (N) uptake was increased by ET, N allocation to the leaves and to Rubisco was reduced under ET and E[CO2] at mid-grain filling, which resulted in a significant decrease (32%) in the maximum rate of ribulose-1,5-bisphosphate carboxylation on a leaf area basis. Because the change in N allocation was associated with the accelerated phenology in E[CO2]-ET plants, we conclude that soil and water warming accelerates photosynthetic down-regulation at E[CO2].

Analysis of a bio-electrochemical reactor containing carbon fiber textiles for the anaerobic digestion of tomato plant residues.

PubMed

Hirano, Shin-Ichi; Matsumoto, Norio

2018-02-01

A bio-electrochemical system packed with supporting material can promote anaerobic digestion for several types of organic waste. To expand the target organic matters of a BES, tomato plant residues (TPRs), generated year-round as agricultural and cellulosic waste, were treated using three methanogenic reactors: a continuous stirred tank reactor (CSTR), a carbon fiber textile (CFT) reactor, and a bio-electrochemical reactor (BER) including CFT with electrochemical regulation (BER + CFT). CFT had positive effects on methane fermentation and methanogen abundance. The microbial population stimulated by electrochemical regulation, including hydrogenotrophic methanogens, cellulose-degrading bacteria, and acetate-degrading bacteria, suppressed acetate accumulation, as evidenced by the low acetate concentration in the suspended fraction in the BER + CFT. These results indicated that the microbial community in the BER + CFT facilitated the efficient decomposition of TPR and its intermediates such as acetate to methane. Copyright © 2017 Elsevier Ltd. All rights reserved.

Increased gibberellin contents contribute to accelerated growth and development of transgenic tobacco overexpressing a wheat ubiquitin gene.

PubMed

Wang, Guo-Kun; Zhang, Meng; Gong, Jiang-Feng; Guo, Qi-Fang; Feng, Ya-Nan; Wang, Wei

2012-12-01

Overexpressing TaUb2 promoted stem growth and resulted in early flowering in transgenic tobacco plants. Ubiquitin are involved in the production, metabolism and proper function of gibberellin. The ubiquitin-26S proteasome system (UPS), in which ubiquitin (Ub) functions as a marker, is a post-translational regulatory system that plays a prominent role in various biological processes. To investigate the impact of different Ub levels on plant growth and development, transgenic tobacco (Nicotiana tabacum L.) plants were engineered to express an Ub gene (TaUb2) from wheat (Triticum aestivum L.) under the control of cauliflower mosaic virus 35S promoter. Transgenic tobacco plants overexpressing TaUb2 demonstrated an accelerated growth rate at early stage and an early flowering phenotype in development. The preceding expression of MADS-box genes also corresponded to the accelerated developmental phenotypes of the transgenic tobacco plants compared to that of wild-type (WT). Total gibberellin (GA) and active GA contents in transgenic tobacco plants were higher than those in WT at the corresponding developmental stages, and some GA metabolism genes were upregulated. Treatment with GA(3) conferred a similarly accelerated grown rate in WT plants to that of transgenic tobacco plants, while growth was inhibited when transgenic tobacco plants were treated with a GA biosynthesis inhibitor. Thus, the results suggest that Ub are involved in the production, metabolism and proper function of GA, which is important in the regulation of plant growth and development.

Automatic external filling for the ion source gas bottle of a Van de Graaff accelerator

NASA Astrophysics Data System (ADS)

Strivay, D.; Bastin, T.; Dehove, C.; Dumont, P. D.; Marchal, A.; Garnir, H.; Weber, G.

1997-09-01

We describe a fully automatic system we developed to fill, from an external gas bottle, the ion source terminal gas storage bottle of a 2 MV Van de Graaff accelerator without depressing the 25 bar insulating gas. The system is based on a programmable automate ordering electropneumatical valves. The only manual operation is the connection of the external gas cylinder. The time needed for a gas change is reduced to typically 15 min (depending on the residual pressure wished for the gas removed from the terminal bottle). To check this system we study the ionic composition of the ion beam delivered by our accelerator after different gas changes. The switching magnet of our accelerator was used to analyse the ionic composition of the accelerated beams in order to verify the degree of elimination of the previous gases in the system.

Protein architecture and core residues in unwound α-helices provide insights to the transport function of plant AtCHX17

DOE Office of Scientific and Technical Information (OSTI.GOV)

Czerny, Daniel D.; Padmanaban, Senthilkumar; Anishkin, Andriy

Using Arabidopsis thaliana AtCHX17 as an example, we combine structural modeling and mutagenesis to provide insights on its protein architecture and transport function which is poorly characterized. This approach is based on the observation that protein structures are significantly more conserved in evolution than linear sequences, and mechanistic similarities among diverse transporters are emerging. Two homology models of AtCHX17 were obtained that show a protein fold similar to known structures of bacterial Na +/H + antiporters, EcNhaA and TtNapA. The distinct secondary and tertiary structure models highlighted residues at positions potentially important for CHX17 activity. Mutagenesis showed that asparagine-N200 andmore » aspartate-D201 inside transmembrane5 (TM5), and lysine-K355 inside TM10 are critical for AtCHX17 activity. We reveal previously unrecognized threonine-T170 and lysine-K383 as key residues at unwound regions in the middle of TM4 and TM11 α-helices, respectively. Mutation of glutamate-E111 located near the membrane surface inhibited AtCHX17 activity, suggesting a role in pH sensing. The long carboxylic tail of unknown purpose has an alternating β-sheet and α-helix secondary structure that is conserved in prokaryote universal stress proteins. Here, these results support the overall architecture of AtCHX17 and identify D201, N200 and novel residues T170 and K383 at the functional core which likely participates in ion recognition, coordination and/or translocation, similar to characterized cation/H + exchangers. The core of AtCHX17 models according to EcNhaA and TtNapA templates faces inward and outward, respectively, which may reflect two conformational states of the alternating access transport mode for proteins belonging to the plant CHX family.« less

Protein architecture and core residues in unwound α-helices provide insights to the transport function of plant AtCHX17

DOE PAGES

Czerny, Daniel D.; Padmanaban, Senthilkumar; Anishkin, Andriy; ...

2016-05-11

Using Arabidopsis thaliana AtCHX17 as an example, we combine structural modeling and mutagenesis to provide insights on its protein architecture and transport function which is poorly characterized. This approach is based on the observation that protein structures are significantly more conserved in evolution than linear sequences, and mechanistic similarities among diverse transporters are emerging. Two homology models of AtCHX17 were obtained that show a protein fold similar to known structures of bacterial Na +/H + antiporters, EcNhaA and TtNapA. The distinct secondary and tertiary structure models highlighted residues at positions potentially important for CHX17 activity. Mutagenesis showed that asparagine-N200 andmore » aspartate-D201 inside transmembrane5 (TM5), and lysine-K355 inside TM10 are critical for AtCHX17 activity. We reveal previously unrecognized threonine-T170 and lysine-K383 as key residues at unwound regions in the middle of TM4 and TM11 α-helices, respectively. Mutation of glutamate-E111 located near the membrane surface inhibited AtCHX17 activity, suggesting a role in pH sensing. The long carboxylic tail of unknown purpose has an alternating β-sheet and α-helix secondary structure that is conserved in prokaryote universal stress proteins. Here, these results support the overall architecture of AtCHX17 and identify D201, N200 and novel residues T170 and K383 at the functional core which likely participates in ion recognition, coordination and/or translocation, similar to characterized cation/H + exchangers. The core of AtCHX17 models according to EcNhaA and TtNapA templates faces inward and outward, respectively, which may reflect two conformational states of the alternating access transport mode for proteins belonging to the plant CHX family.« less

Residual phytotoxicity of parthenium: Impact on some winter crops, weeds and soil properties.

PubMed

Khaliq, Abdul; Aslam, Farhena; Matloob, Amar; Hussain, Saddam; Tanveer, Asif; Alsaadawi, Ibrahim; Geng, Mingjian

2015-12-01

Phytotoxic effects of parthenium residues incorporation and parthenium-infested rhizospheric soil on emergence and seedling growth of winter crops (wheat and canola) and weed species (wild oat and canary grass) were examined in different pot studies. In first experiment, parthenium whole plant residues were incorporated at 6 and 8 g kg(-1) soil five days prior to sowing. Pots without residues incorporation were maintained as control. In a second study, parthenium-infested rhizospheric soil collected from different depths (15 and 22.5 cm) and collar regions (horizontal distance away from plant trunk, 15 and 22.5 cm), was used as growing medium. Parthenium-free soil was used as control. Parthenium residues amendment as well as its rhizospheric soil was detrimental for emergence and seedling growth of all test species. Incorporation of parthenium residues reduced the final emergence of canola, wild oat and canary grass by 11-20%, 20-29% and 20-27%, respectively; however wheat emergence was unaffected. Moreover, seedling biomass of wheat, canola, wild oat and canary grass was reduced in the range of 41-48%, 53-61%, 31-45% and 30-45% by parthenium residues incorporation. In second study, soil collected from a rhizospheric depth of 15 cm and collar distance of 15 cm reduced the emergence and seedling growth by 15% and 40%, respectively averaged across different test species. Parthenium residues incorporation and infested rhizospheric soil increased the soil phenolics, electrical conductivity, organic carbon and nitrogen contents over control soils with the exception of pH that was declined. All test species manifested reduced chlorophyll and increased phenolic contents in response to parthenium residues incorporation and infested rhizospheric soil. The inhibition in emergence and seedling growth of all test species was associated with increase in phenolic contents. Parthenium residues incorporation at 8 g kg(-1) soil and upper parthenium-infested rhizospheric soil (15 cm

A statistical investigation of the mass discrepancy-acceleration relation

NASA Astrophysics Data System (ADS)

Desmond, Harry

2017-02-01

We use the mass discrepancy-acceleration relation (the correlation between the ratio of total-to-visible mass and acceleration in galaxies; MDAR) to test the galaxy-halo connection. We analyse the MDAR using a set of 16 statistics that quantify its four most important features: shape, scatter, the presence of a `characteristic acceleration scale', and the correlation of its residuals with other galaxy properties. We construct an empirical framework for the galaxy-halo connection in LCDM to generate predictions for these statistics, starting with conventional correlations (halo abundance matching; AM) and introducing more where required. Comparing to the SPARC data, we find that: (1) the approximate shape of the MDAR is readily reproduced by AM, and there is no evidence that the acceleration at which dark matter becomes negligible has less spread in the data than in AM mocks; (2) even under conservative assumptions, AM significantly overpredicts the scatter in the relation and its normalization at low acceleration, and furthermore positions dark matter too close to galaxies' centres on average; (3) the MDAR affords 2σ evidence for an anticorrelation of galaxy size and Hubble type with halo mass or concentration at fixed stellar mass. Our analysis lays the groundwork for a bottom-up determination of the galaxy-halo connection from relations such as the MDAR, provides concrete statistical tests for specific galaxy formation models, and brings into sharper focus the relative evidence accorded by galaxy kinematics to LCDM and modified gravity alternatives.

Residues in Beeswax: A Health Risk for the Consumer of Honey and Beeswax?

PubMed

Wilmart, Olivier; Legrève, Anne; Scippo, Marie-Louise; Reybroeck, Wim; Urbain, Bruno; de Graaf, Dirk C; Steurbaut, Walter; Delahaut, Philippe; Gustin, Pascal; Nguyen, Bach Kim; Saegerman, Claude

2016-11-09

A scenario analysis in regard to the risk of chronic exposure of consumers to residues through the consumption of contaminated honey and beeswax was conducted. Twenty-two plant protection products and veterinary substances of which residues have already been detected in beeswax in Europe were selected. The potential chronic exposure was assessed by applying a worst-case scenario based on the addition of a "maximum" daily intake through the consumption of honey and beeswax to the theoretical maximum daily intake through other foodstuffs. For each residue, the total exposure was finally compared to the acceptable daily intake. It is concluded that the food consumption of honey and beeswax contaminated with these residues considered separately does not compromise the consumer's health, provided proposed action limits are met. In regard to residues of flumethrin in honey and in beeswax, "zero tolerance" should be applied.

Cleanup procedure for water, soil, animal and plant extracts for the use of electron-capture detector in the gas chromatographic analysis of organophosphorus insecticide residues.

PubMed

Kadoum, A M

1968-07-01

A simple, aqueous acetonitrile partition cleanup method for analyses of some common organophosphorus insecticide residues is described. The procedure described is for cleanup and quantitative recovery of parathion, methyl parathion, diazinon, malathion and thimet from different extracts. Those insecticides in the purified extracts of ground water, grain, soil, plant and animal tissues can be detected quantitatively by gas chromatography with an electron capture-detector at 0.01 ppm. Cleanup is satisfactory for paper and thin-layer chromatography for further identification of individual insecticides in the extracts.

Determination of Arsenical Herbicide Residues in Plant Tissues

Treesearch

R.M. Sachs; J.L. Michael; F.B. Anastasia; W.A. Wells

1971-01-01

Paper chromatographic separation of hydroxydimethylarsine oxide (cacodylic acid), monosodium methanearsonate (MSMA), sodium arsenate, and sodium arsenite was achieved with the aid of four solvent systems. Aqueous extracts of plant tissues removed essentially all the arscnicals applied, but mechanoiic fractionation was required before the extracts could be analyzed by...

Accelerator system and method of accelerating particles

NASA Technical Reports Server (NTRS)

Wirz, Richard E. (Inventor)

2010-01-01

An accelerator system and method that utilize dust as the primary mass flux for generating thrust are provided. The accelerator system can include an accelerator capable of operating in a self-neutralizing mode and having a discharge chamber and at least one ionizer capable of charging dust particles. The system can also include a dust particle feeder that is capable of introducing the dust particles into the accelerator. By applying a pulsed positive and negative charge voltage to the accelerator, the charged dust particles can be accelerated thereby generating thrust and neutralizing the accelerator system.

Splash-cup plants accelerate raindrops to disperse seeds

PubMed Central

Amador, Guillermo J.; Yamada, Yasukuni; McCurley, Matthew; Hu, David L.

2013-01-01

The conical flowers of splash-cup plants Chrysosplenium and Mazus catch raindrops opportunistically, exploiting the subsequent splash to disperse their seeds. In this combined experimental and theoretical study, we elucidate their mechanism for maximizing dispersal distance. We fabricate conical plant mimics using three-dimensional printing, and use high-speed video to visualize splash profiles and seed travel distance. Drop impacts that strike the cup off-centre achieve the largest dispersal distances of up to 1 m. Such distances are achieved because splash speeds are three to five times faster than incoming drop speeds, and so faster than the traditionally studied splashes occurring upon horizontal surfaces. This anomalous splash speed is because of the superposition of two components of momentum, one associated with a component of the drop's motion parallel to the splash-cup surface, and the other associated with film spreading induced by impact with the splash-cup. Our model incorporating these effects predicts the observed dispersal distance within 6–18% error. According to our experiments, the optimal cone angle for the splash-cup is 40°, a value consistent with the average of five species of splash-cup plants. This optimal angle arises from the competing effects of velocity amplification and projectile launching angle. PMID:23235266

Splash-cup plants accelerate raindrops to disperse seeds.

PubMed

Amador, Guillermo J; Yamada, Yasukuni; McCurley, Matthew; Hu, David L

2013-02-01

The conical flowers of splash-cup plants Chrysosplenium and Mazus catch raindrops opportunistically, exploiting the subsequent splash to disperse their seeds. In this combined experimental and theoretical study, we elucidate their mechanism for maximizing dispersal distance. We fabricate conical plant mimics using three-dimensional printing, and use high-speed video to visualize splash profiles and seed travel distance. Drop impacts that strike the cup off-centre achieve the largest dispersal distances of up to 1 m. Such distances are achieved because splash speeds are three to five times faster than incoming drop speeds, and so faster than the traditionally studied splashes occurring upon horizontal surfaces. This anomalous splash speed is because of the superposition of two components of momentum, one associated with a component of the drop's motion parallel to the splash-cup surface, and the other associated with film spreading induced by impact with the splash-cup. Our model incorporating these effects predicts the observed dispersal distance within 6-18% error. According to our experiments, the optimal cone angle for the splash-cup is 40°, a value consistent with the average of five species of splash-cup plants. This optimal angle arises from the competing effects of velocity amplification and projectile launching angle.

Development of advanced technological systems for accelerator transmutation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Batskikh, G.I.; Bondarev, B.I.; Durkin, A.P.

1995-10-01

A development concept of the accelerator nuclear energy reactors is considered for energy generation and nuclear power plant waste conversion into short-lived nuclides along with the requirements imposed on the technological systems necessary for implementation of such projects. The state of art in the field is discussed.

Performance of some miniature pressure transducers subjected to high rotational speeds and centripetal accelerations

NASA Technical Reports Server (NTRS)

Minkin, H. L.

1976-01-01

The performance characteristics of several miniature pressure transducers were determined at centripetal accelerations up to 11,200 g's at a rotational speed of 23,000 rpm. The variation in centripetal acceleration was produced by changing radial position of the transducer relative to the center of rotation. Residual zero outputs and transducer sensitivities were determined at 23,000 rpm and compared with those determined at 0 rpm. The actual pressures at the various transducer locations differ from the center line impressed pressures due to a rotational effect. Corrections for this effect were made. A brief description of the test apparatus is included.

JAERI R & D on accelerator-based transmutation under OMEGA program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Takizuka, T.; Nishida, T.; Mizumoto, M.

1995-10-01

The overview of the Japanese long-term research and development program on nuclide partitioning and transmutation, called {open_quotes}OMEGA,{close_quotes} is presented. Under this national program, major R&D activities are being carried out at JAERI, PNC, and CRIEPI. Accelerator-based transmutation study at JAERI is focused on a dedicated transmutor with a subcritical actinide-fueled subcritical core coupled with a spallation target driven by a high intensity proton accelerator. Two types of system concept, solid system and molten-salt system, are discussed. The solid system consists of sodium-cooled tungsten target and metallic actinide fuel. The molten-salt system is fueled with molten actinide chloride that acts alsomore » as a target material. The proposed plant transmutes about 250 kg of minor actinide per year, and generates enough electricity to power its own accelerator. JAERI is proposing the development of an intense proton linear accelerator ETA with 1.5 GeV-10 mA beam for engineering tests of accelerator-based transmutation. Recent achievements in the accelerator development are described.« less

Atomic structure of recombinant thaumatin II reveals flexible conformations in two residues critical for sweetness and three consecutive glycine residues.

PubMed

Masuda, Tetsuya; Mikami, Bunzo; Tani, Fumito

2014-11-01

Thaumatin, an intensely sweet-tasting protein used as a sweetener, elicits a sweet taste at 50 nM. Although two major variants designated thaumatin I and thaumatin II exist in plants, there have been few dedicated thaumatin II structural studies and, to date, data beyond atomic resolution had not been obtained. To identify the detailed structural properties explaining why thaumatin elicits a sweet taste, the structure of recombinant thaumatin II was determined at the resolution of 0.99 Å. Atomic resolution structural analysis with riding hydrogen atoms illustrated the differences in the direction of the side-chains more precisely and the electron density maps of the C-terminal regions were markedly improved. Though it had been suggested that the three consecutive glycine residues (G142-G143-G144) have highly flexible conformations, G143, the central glycine residue was successfully modelled in two conformations for the first time. Furthermore, the side chain r.m.s.d. values for two residues (R67 and R82) critical for sweetness exhibited substantially higher values, suggesting that these residues are highly disordered. These results demonstrated that the flexible conformations in two critical residues favoring their interaction with sweet taste receptors are prominent features of the intensely sweet taste of thaumatin. Copyright © 2014 Elsevier B.V. and Société française de biochimie et biologie Moléculaire (SFBBM). All rights reserved.

Evaluation of resource recovery from waste incineration residues--the case of zinc.

PubMed

Fellner, J; Lederer, J; Purgar, A; Winterstetter, A; Rechberger, H; Winter, F; Laner, D

2015-03-01

Solid residues generated at European Waste to Energy plants contain altogether about 69,000 t/a of Zn, of which more than 50% accumulates in air pollution control residues, mainly boiler and filter ashes. Intensive research activities aiming at Zn recovery from such residues recently resulted in a technical scale Zn recovery plant at a Swiss waste incinerator. By acidic leaching and subsequent electrolysis this technology (FLUREC) allows generating metallic Zn of purity>99.9%. In the present paper the economic viability of the FLUREC technology with respect to Zn recovery from different solid residues of waste incineration has been investigated and subsequently been categorised according to the mineral resource classification scheme of McKelvey. The results of the analysis demonstrate that recovery costs for Zn are highly dependent on the costs for current fly ash disposal (e.g. cost for subsurface landfilling). Assuming current disposal practice costs of 220€/ton fly ash, resulting recovery costs for Zn are generally higher than its current market price of 1.6€/kg Zn. With respect to the resource classification this outcome indicates that none of the identified Zn resources present in incineration residues can be economically extracted and thus cannot be classified as a reserve. Only for about 4800 t/a of Zn an extraction would be marginally economic, meaning that recovery costs are only slightly (less than 20%) higher than the current market price for Zn. For the remaining Zn resources production costs are between 1.5 and 4 times (7900 t/a Zn) and 10-80 times (55,300 t/a Zn) higher than the current market value. The economic potential for Zn recovery from waste incineration residues is highest for filter ashes generated at grate incinerators equipped with wet air pollution control. Copyright © 2014 Elsevier Ltd. All rights reserved.

Occurrence of pesticide residues in fruiting vegetables from production farms in south-eastern region of Poland

PubMed

Słowik-Borowiec, Magdalena; Szpyrka, Ewa; Rupar, Julian; Podbielska, Magdalena; Matyaszek, Aneta

Considering the fact that pesticides are commonly used in agriculture, continuous monitoring of these substances in food products is of great significance. Residues of these substances can be present in crops after harvest. The aim of this study was to evaluate presence of pesticide residues in fruiting vegetables from production farms in south-eastern region of Poland in 2012–2015. 138 samples were tested using accredited test methods. The monitoring programme covered determination of 242 pesticides. The tests covered tomato, cucumber and pepper crops. The test results were interpreted in accordance with criteria included in the European Commission recommendations published in the document SANCO/12571/2013 (now superseded by Document SANTE 2015), as well as on a basis of the maximum residue levels in force in the EU Member States. Pesticide residues were found in 47 samples, representing 34% of all tested samples. 17 active substances were found, belonging to fungicides and insecticides. Azoxystrobin (38%), boscalid (28%) and chlorothalonil (21%) were most commonly found in fruiting vegetables testing samples. Non-compliances related to use of plant protection product not authorized for protection of a given crop were observed in 6% of analysed samples. However, pesticide residues of fruiting vegetables in quantities that exceed the maximum residue levels (NDP, ang. MRLs), as well as substances which use for plant protection is forbidden were no found. Crops monitoring is used to determine to what extent such products are contaminated with pesticide residues, and ensures protection of customer health.

Characterization of Chlamydomonas Ribulose-1,5-bisphosphate carboxylase/oxygenase variants mutated at residues that are post-translationally modified.

PubMed

Rasineni, Girish Kumar; Loh, Pek Chin; Lim, Boon Hoe

2017-02-01

Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the chloroplast enzyme that fixes CO 2 in photosynthesis, but the enzyme also fixes O 2 , which leads to the wasteful photorespiratory pathway. If we better understand the structure-function relationship of the enzyme, we might be able to engineer improvements. When the crystal structure of Chlamydomonas Rubisco was solved, four new posttranslational modifications were observed which are not present in other species. The modifications were 4-hydroxylation of the conserved Pro-104 and 151 residues, and S-methylation of the variable Cys-256 and 369 residues, which are Phe-256 and Val-369 in land plants. Because the modifications were only observed in Chlamydomonas Rubisco, they might account for the differences in kinetic properties between the algal and plant enzymes. Site-directed mutagenesis and chloroplast transformation have been used to test the essentiality of these modifications by replacing each of the residues with alanine (Ala). Biochemical analyses were done to determine the specificity factors and kinetic constants. Replacing the modified-residues in Chlamydomonas Rubisco affected the enzyme's catalytic activity. Substituting hydroxy-Pro-104 and methyl-Cys-256 with alanine influenced Rubisco catalysis. This is the first study on these posttranslationally-modified residues in Rubisco by genetic engineering. As these forms of modifications/regulation are not available in plants, the modified residues could be a means to modulate Rubisco activity. With a better understanding of Rubisco structure-function, we can define targets for improving the enzyme. Copyright © 2016 Elsevier B.V. All rights reserved.

Brassinosteroids play a critical role in the regulation of pesticide metabolism in crop plants

PubMed Central

Zhou, Yanhong; Xia, Xiaojian; Yu, Gaobo; Wang, Jitao; Wu, Jingxue; Wang, Mengmeng; Yang, Youxin; Shi, Kai; Yu, Yunlong; Chen, Zhixiang; Gan, Jay; Yu, Jingquan

2015-01-01

Pesticide residues in agricultural produce pose a threat to human health worldwide. Although the detoxification mechanisms for xenobiotics have been extensively studied in mammalian cells, information about the regulation network in plants remains elusive. Here we show that brassinosteroids (BRs), a class of natural plant hormones, decreased residues of common organophosphorus, organochlorine and carbamate pesticides by 30–70% on tomato, rice, tea, broccoli, cucumber, strawberry, and other plants when treated externally. Genome-wide microarray analysis showed that fungicide chlorothalonil (CHT) and BR co-upregulated 301 genes, including a set of detoxifying genes encoding cytochrome P450, oxidoreductase, hydrolase and transferase in tomato plants. The level of BRs was closely related to the respiratory burst oxidase 1 (RBOH1)-encoded NADPH oxides-dependent H2O2 production, glutathione biosynthesis and the redox homeostasis, and the activity of glutathione S-transferase (GST). Gene silencing treatments showed that BRs decreased pesticide residues in plants likely by promoting their metabolism through a signaling pathway involving BRs-induced H2O2 production and cellular redox change. Our study provided a novel approach for minimizing pesticide residues in crops by exploiting plants' own detoxification mechanisms. PMID:25761674

Numerical and Experimental Investigation of the Effects of Acceleration Disturbances on Microgravity Experiments

NASA Technical Reports Server (NTRS)

Ramachandran, Narayanan

2000-01-01

Normal vibrational modes on large spacecraft are excited by crew activity, operating machinery, and other mechanical disturbances. Periodic engine burns for maintaining vehicle attitude and random impulse type disturbances also contribute to the acceleration environment of a Spacecraft. Accelerations from these vibrations (often referred to as g-jitter) are several orders of magnitude larger than the residual accelerations from atmospheric drag and gravity gradient effects. Naturally, the effects of such accelerations have been a concern to prospective experimenters wishing to take advantage of the microgravity environment offered by spacecraft operating in low Earth orbit and the topic has been studied extensively, both numerically and analytically. However, these studies have not produced a general theory that predicts the effects of multi-spectral periodic accelerations on a general class of experiments nor have they produced scaling laws that a prospective experimenter could use to assess how his/her experiment might be affected by this acceleration environment. Furthermore, there are no actual flight experimental data that correlates heat or mass transport with measurements of the periodic acceleration environment. The present investigation approaches this problem with carefully conducted terrestrial experiments and rigorous numerical modeling thereby providing comparative theoretical and experimental data. The modeling, it is hoped will provide a predictive tool that can be used for assessing experiment response to Spacecraft vibrations.

Recycling crop residues for use in recirculating hydroponic crop production

NASA Technical Reports Server (NTRS)

Mackowiak, C. L.; Garland, J. L.; Sager, J. C.

1996-01-01

As part of bioregenerative life support feasibility testing by NASA, crop residues are being used to resupply elemental nutrients to recirculating hydroponic crop production systems. Methods for recovering nutrients from crop residues have evolved from water soaking (leaching) to rapid aerobic bioreactor processing. Leaching residues recovered the majority of elements but it also recovered significant amounts of soluble organics. The high organic content of leachates was detrimental to plant growth. Aerobic bioreactor processing reduced the organic content ten-fold, which reduced or eliminated phytotoxic effects. Wheat and potato production studies were successful using effluents from reactors having with 8- to 1-day retention times. Aerobic bioreactor effluents supplied at least half of the crops elemental mass needs in these studies. Descriptions of leachate and effluent mineral content, biomass productivity, microbial activity, and nutrient budgets for potato and wheat are presented.

Two-dimensional coordination polymer matrix for solid-phase extraction of pesticide residues from plant Cordia salicifolia.

PubMed

de Carvalho, Pedro Henrique Viana; Barreto, Alysson Santos; Rodrigues, Marcelo O; Prata, Vanessa de Menezes; Alves, Péricles Barreto; de Mesquita, Maria Eliane; Alves, Severino; Navickiene, Sandro

2009-06-01

The 2D coordination polymer (infinity[Gd(DPA)(HDPA)]) was tested for extraction of acephate, chlorpropham, pirimicarb, bifenthrin, tetradifon, and phosalone from the medicinal plant Cordia salicifolia, whose extracts are commercialized in Brazil as diuretic, appetite suppressant, and weight loss products, using GC/MS, SIM. Considering that there are no Brazilian regulations concerning maximum permissible pesticide residue concentrations in medicinal herbs, recovery experiments were carried out (seven replicates), at two arbitrary fortification levels (0.5 and 1.0 mg/kg), resulting in recoveries in range of 20 to 107.7% and SDRSDs were between 5.6 and 29.1% for infinity[Gd(DPA)(HDPA)] sorbent. Detection and quantification limits for herb ranged from 0.10 to 0.15 mg/kg and from 0.15 to 0.25 mg/kg, respectively, for the different pesticides studied. The developed method is linear over the range assayed, 0.5-10.0 microg/mL, with correlation coefficients ranging from 0.9975 to 0.9986 for all pesticides. Comparison between infinity[Gd(DPA)(HDPA)] sorbent and conventional sorbent (neutral alumina) showed similar performance of infinity[Gd(DPA)(HDPA)] polymeric sorbent for three (bifenthrin, tetradifon, and phosalone) out of six pesticides tested.

Speed breeding is a powerful tool to accelerate crop research and breeding.

PubMed

Watson, Amy; Ghosh, Sreya; Williams, Matthew J; Cuddy, William S; Simmonds, James; Rey, María-Dolores; Asyraf Md Hatta, M; Hinchliffe, Alison; Steed, Andrew; Reynolds, Daniel; Adamski, Nikolai M; Breakspear, Andy; Korolev, Andrey; Rayner, Tracey; Dixon, Laura E; Riaz, Adnan; Martin, William; Ryan, Merrill; Edwards, David; Batley, Jacqueline; Raman, Harsh; Carter, Jeremy; Rogers, Christian; Domoney, Claire; Moore, Graham; Harwood, Wendy; Nicholson, Paul; Dieters, Mark J; DeLacy, Ian H; Zhou, Ji; Uauy, Cristobal; Boden, Scott A; Park, Robert F; Wulff, Brande B H; Hickey, Lee T

2018-01-01

The growing human population and a changing environment have raised significant concern for global food security, with the current improvement rate of several important crops inadequate to meet future demand 1 . This slow improvement rate is attributed partly to the long generation times of crop plants. Here, we present a method called 'speed breeding', which greatly shortens generation time and accelerates breeding and research programmes. Speed breeding can be used to achieve up to 6 generations per year for spring wheat (Triticum aestivum), durum wheat (T. durum), barley (Hordeum vulgare), chickpea (Cicer arietinum) and pea (Pisum sativum), and 4 generations for canola (Brassica napus), instead of 2-3 under normal glasshouse conditions. We demonstrate that speed breeding in fully enclosed, controlled-environment growth chambers can accelerate plant development for research purposes, including phenotyping of adult plant traits, mutant studies and transformation. The use of supplemental lighting in a glasshouse environment allows rapid generation cycling through single seed descent (SSD) and potential for adaptation to larger-scale crop improvement programs. Cost saving through light-emitting diode (LED) supplemental lighting is also outlined. We envisage great potential for integrating speed breeding with other modern crop breeding technologies, including high-throughput genotyping, genome editing and genomic selection, accelerating the rate of crop improvement.

Cation and anion leaching and growth of Acacia saligna in bauxite residue sand amended with residue mud, poultry manure and phosphogypsum.

PubMed

Jones, B E H; Haynes, R J; Phillips, I R

2012-03-01

To examine (1) the effect of organic (poultry manure) and inorganic (residue mud and phosphogypsum) amendments on nutrient leaching losses from residue sand and (2) whether amendments improve the growth of plants in residue sand. Leaching columns were established using residue sand. The phosphogypsum-treated surface layer (0-15 cm) was amended with poultry manure and/or bauxite residue mud and the subsurface layer (15-45 cm) was either left untreated or amended with phosphogypsum. Much of the Na⁺, K⁺, Cl⁻ and SO₄²⁻ was lost during the first four leachings. Additions of phosphogypsum to both surface and subsurface layers resulted in partial neutralization of soluble alkalinity. Mean pH of leachates ranged from 8.0 to 8.4, the major cation leached was Na⁺ and the major balancing anion was SO₄²⁻ . Where gypsum was not applied to the subsurface, mean pH of leachates was 10.0-10.9, the main cation leached was still Na⁺ and the main balancing anions were a combination of SO₄²⁻ and HCO₃⁻/CO₃²⁻. At the end of the experiment, concentrations of exchangeable Na⁺ in the subsurface layers were similar regardless of whether gypsum had been applied to that layer or not. Yields of Acacia saligna were promoted by additions of poultry manure to the surface layer but unaffected by gypsum incorporation into the subsurface layer. Lack of reaction of phosphogypsum with the subsurface layer is unlikely to be a major factor limiting revegetation of residue sand since in the absence of phosphogypsum the excess Na⁺ leaches with the residual alkalinity (HCO₃⁻/CO₃²⁻) rather than SO₄²⁻.

Activated carbon for the removal of pharmaceutical residues from treated wastewater.

PubMed

Ek, Mats; Baresel, Christian; Magnér, Jörgen; Bergström, Rune; Harding, Mila

2014-01-01

Pharmaceutical residues, which pass naturally through the human body into sewage, are in many cases virtually unaffected by conventional wastewater treatment. Accumulated in the environment, however, they can significantly impact aquatic life. The present study indicates that many pharmaceutical residues found in wastewater can be removed with activated carbon in a cost-efficient system that delivers higher resource utilisation and security than other carbon systems. The experiment revealed a substantial separation of the analysed compounds, notwithstanding their relatively high solubility in water and dissimilar chemical structures. This implies that beds of activated carbon may be a competitive alternative to treatment with ozone. The effluent water used for the tests, performed over 20 months, originated from Stockholm's largest sewage treatment plant. Passing through a number of different filters with activated carbon removed 90-98% of the pharmaceutical residues from the water. This paper describes pilot-scale tests performed by IVL and the implications for an actual treatment plant that has to treat up to several thousand litres of wastewater per second. In addition, the advantages, disadvantages and costs of the method are discussed. This includes, for example, the clogging of carbon filters and the associated hydraulic capacity limits of the activated carbon.

Simultaneous determination of three pesticide adjuvant residues in plant-derived agro-products using liquid chromatography-tandem mass spectrometry.

PubMed

Li, Hui; Jiang, Zejun; Cao, Xiaolin; Su, Hang; Shao, Hua; Jin, Fen; Abd El-Aty, A M; Wang, Jing

2017-12-15

Herein, an accurate and reliable isotope-labelled internal standard method was developed and validated for simultaneous determination of three polar pesticide adjuvants, namely 2-pyrrolidone, N-methyl-2-pyrrolidone, and N-ethyl-2-pyrrolidone in plant-derived agro-products. Matrices, including apple, cabbage, tomato, cucumber, rice, and wheat were extracted with a modified quick, easy, cheap, effective, rugged, and safe "QuEChERS" method and purified with a new clean-up sorbent (Z-Sep). A hydrophilic interaction liquid chromatography column (HILIC), exhibiting a lipophilic-hydrophilic character, was used to separate the three analytes over 10min using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Matrix effects in various matrices were evaluated and an isotope-labelled internal standard method was employed to compensate for ion enhancement/suppression effects. At three fortification levels (2.0, 5.0, and 20.0μg/kg), the mean recoveries ranged from 78.5 to 112.1% with relative standard deviations (RSDs)<11.0% for all tested analytes. The limits of detection (LODs) and quantification (LOQs) were 0.04-0.45 and 0.12-1.58μg/kg in various matrices, respectively. The developed experimental protocol was successfully applied to monitor different samples purchased from local markets in Beijing, China. In conclusion, the developed method exhibited both high sensitivity and satisfactory accuracy and is suitable for the simultaneous determination of the three tested pesticide adjuvant residues in agro-products of plant origin. Copyright © 2017 Elsevier B.V. All rights reserved.

Trends in the management of residual municipal solid waste.

PubMed

Rada, E C; Istrate, I A; Ragazzi, M

2009-06-01

In agreement with European Union directives, the integrated management of municipal solid waste must be developed ensuring a balanced relationship between the streams of selective collection and the one regarding the residual waste. A theoretical scenario is made where the residual municipal solid waste is composed only of non-recyclable fractions. An important aspect concerns the role of the organic fraction as selective collection can significantly decrease its content in the residual waste. This paper focuses on the planning, design and management consequences of this unsteady scenario. The treatments that are considered are: combustion, gasification, pyrolysis, integrated thermal plants, aerobic mechanical-biological treatments, anaerobic mechanical-biological treatments and other types of treatment. The considerations are based on the experience of the authors not only in terms of development of research but also in terms of transfer of the research results to the real scale, and knowledge of the state-of-the-art of the sector.

A statistical investigation of the mass discrepancy–acceleration relation

DOE PAGES

Desmond, Harry

2016-10-08

We use the mass discrepancy–acceleration relation (the correlation between the ratio of total-to-visible mass and acceleration in galaxies; MDAR) to test the galaxy–halo connection. Here, we analyse the MDAR using a set of 16 statistics that quantify its four most important features: shape, scatter, the presence of a ‘characteristic acceleration scale’, and the correlation of its residuals with other galaxy properties. We construct an empirical framework for the galaxy–halo connection in LCDM to generate predictions for these statistics, starting with conventional correlations (halo abundance matching; AM) and introducing more where required. Comparing to the SPARC data, we find that: (1)more » the approximate shape of the MDAR is readily reproduced by AM, and there is no evidence that the acceleration at which dark matter becomes negligible has less spread in the data than in AM mocks; (2) even under conservative assumptions, AM significantly overpredicts the scatter in the relation and its normalization at low acceleration, and furthermore positions dark matter too close to galaxies’ centres on average; (3) the MDAR affords 2σ evidence for an anticorrelation of galaxy size and Hubble type with halo mass or concentration at fixed stellar mass. Lastly, our analysis lays the groundwork for a bottom-up determination of the galaxy–halo connection from relations such as the MDAR, provides concrete statistical tests for specific galaxy formation models, and brings into sharper focus the relative evidence accorded by galaxy kinematics to LCDM and modified gravity alternatives.« less

Plastid–Nuclear Interaction and Accelerated Coevolution in Plastid Ribosomal Genes in Geraniaceae

PubMed Central

Weng, Mao-Lun; Ruhlman, Tracey A.; Jansen, Robert K.

2016-01-01

Plastids and mitochondria have many protein complexes that include subunits encoded by organelle and nuclear genomes. In animal cells, compensatory evolution between mitochondrial and nuclear-encoded subunits was identified and the high mitochondrial mutation rates were hypothesized to drive compensatory evolution in nuclear genomes. In plant cells, compensatory evolution between plastid and nucleus has rarely been investigated in a phylogenetic framework. To investigate plastid–nuclear coevolution, we focused on plastid ribosomal protein genes that are encoded by plastid and nuclear genomes from 27 Geraniales species. Substitution rates were compared for five sets of genes representing plastid- and nuclear-encoded ribosomal subunit proteins targeted to the cytosol or the plastid as well as nonribosomal protein controls. We found that nonsynonymous substitution rates (dN) and the ratios of nonsynonymous to synonymous substitution rates (ω) were accelerated in both plastid- (CpRP) and nuclear-encoded subunits (NuCpRP) of the plastid ribosome relative to control sequences. Our analyses revealed strong signals of cytonuclear coevolution between plastid- and nuclear-encoded subunits, in which nonsynonymous substitutions in CpRP and NuCpRP tend to occur along the same branches in the Geraniaceae phylogeny. This coevolution pattern cannot be explained by physical interaction between amino acid residues. The forces driving accelerated coevolution varied with cellular compartment of the sequence. Increased ω in CpRP was mainly due to intensified positive selection whereas increased ω in NuCpRP was caused by relaxed purifying selection. In addition, the many indels identified in plastid rRNA genes in Geraniaceae may have contributed to changes in plastid subunits. PMID:27190001

Hazardous properties of paint residues from the furniture industry.

PubMed

Vaajasaari, Kati; Kulovaara, Maaret; Joutti, Anneli; Schultz, Eija; Soljamo, Kari

2004-01-30

The objective of this study was to screen nine excess paint residues for environmental hazard and to evaluate their disposability in a non-hazardous or hazardous-waste landfill. These residues were produced in the process of spray-painting furniture. Residues were classified according to their leaching and ecotoxicological properties. Leaching properties were determined with the European standard SFS-EN 12457-2 leaching-test. The toxicity of the leaching-test eluates was measured with plant-, bacteria- and enzyme-inhibition bioassays. Total organic carbon, formaldehyde and solvent concentrations in the solid wastes and in the leaching-test eluates were analysed. It seemed likely that leached formaldehyde caused very high acute toxicity in leaching-test eluates of the dry-booth residues. This hypothesis was based on the fact that the formaldehyde concentrations in the leaching-test eluates of the dry-booth residues were 62-75 times higher than the EC50 value reported in the literature for formaldehyde. The results of the water-curtain booth residues showed that the samples with the highest TOC and aromatic solvent concentrations were also the most toxic. The studied excess paint residues were complex organic mixtures and contained large amounts of compounds not identifiable from chemical data. Therefore, the evaluation of the hazard based solely on available chemical data is unlikely to be sufficient, as evidenced by our study. Our results show that harmful compounds remain in the solid waste and the toxicity results of their leaching-test eluates show that toxicity may leach from residues in contact with water at landfill sites. They also confirm the benefit of combining chemical and ecotoxicological assays in assessing the potential environmental hazard of complex organic mixtures found in wastes. Copyright 2003 Elsevier B.V.

Pesticide Residues and Bees – A Risk Assessment

PubMed Central

Sanchez-Bayo, Francisco; Goka, Koichi

2014-01-01

Bees are essential pollinators of many plants in natural ecosystems and agricultural crops alike. In recent years the decline and disappearance of bee species in the wild and the collapse of honey bee colonies have concerned ecologists and apiculturalists, who search for causes and solutions to this problem. Whilst biological factors such as viral diseases, mite and parasite infections are undoubtedly involved, it is also evident that pesticides applied to agricultural crops have a negative impact on bees. Most risk assessments have focused on direct acute exposure of bees to agrochemicals from spray drift. However, the large number of pesticide residues found in pollen and honey demand a thorough evaluation of all residual compounds so as to identify those of highest risk to bees. Using data from recent residue surveys and toxicity of pesticides to honey and bumble bees, a comprehensive evaluation of risks under current exposure conditions is presented here. Standard risk assessments are complemented with new approaches that take into account time-cumulative effects over time, especially with dietary exposures. Whilst overall risks appear to be low, our analysis indicates that residues of pyrethroid and neonicotinoid insecticides pose the highest risk by contact exposure of bees with contaminated pollen. However, the synergism of ergosterol inhibiting fungicides with those two classes of insecticides results in much higher risks in spite of the low prevalence of their combined residues. Risks by ingestion of contaminated pollen and honey are of some concern for systemic insecticides, particularly imidacloprid and thiamethoxam, chlorpyrifos and the mixtures of cyhalothrin and ergosterol inhibiting fungicides. More attention should be paid to specific residue mixtures that may result in synergistic toxicity to bees. PMID:24718419

Molecular evolution accompanying functional divergence of duplicated genes along the plant starch biosynthesis pathway

PubMed Central

2014-01-01

Background Starch is the main source of carbon storage in the Archaeplastida. The starch biosynthesis pathway (sbp) emerged from cytosolic glycogen metabolism shortly after plastid endosymbiosis and was redirected to the plastid stroma during the green lineage divergence. The SBP is a complex network of genes, most of which are members of large multigene families. While some gene duplications occurred in the Archaeplastida ancestor, most were generated during the sbp redirection process, and the remaining few paralogs were generated through compartmentalization or tissue specialization during the evolution of the land plants. In the present study, we tested models of duplicated gene evolution in order to understand the evolutionary forces that have led to the development of SBP in angiosperms. We combined phylogenetic analyses and tests on the rates of evolution along branches emerging from major duplication events in six gene families encoding sbp enzymes. Results We found evidence of positive selection along branches following cytosolic or plastidial specialization in two starch phosphorylases and identified numerous residues that exhibited changes in volume, polarity or charge. Starch synthases, branching and debranching enzymes functional specializations were also accompanied by accelerated evolution. However, none of the sites targeted by selection corresponded to known functional domains, catalytic or regulatory. Interestingly, among the 13 duplications tested, 7 exhibited evidence of positive selection in both branches emerging from the duplication, 2 in only one branch, and 4 in none of the branches. Conclusions The majority of duplications were followed by accelerated evolution targeting specific residues along both branches. This pattern was consistent with the optimization of the two sub-functions originally fulfilled by the ancestral gene before duplication. Our results thereby provide strong support to the so-called “Escape from Adaptive Conflict�

Land application of organic residuals: Public health threat or environmental benefit

USDA-ARS?s Scientific Manuscript database

Waste residuals consist of manure and biosolids produced by concentrated animal feeding operations and municipal waste water treatment plants. All wastes need to be disposed of in a proper manner, protecting public and environmental health, but also in a sustainable fashion to ensure that no system...

Prediction of residual shear strength of corroded reinforced concrete beams

NASA Astrophysics Data System (ADS)

Imam, Ashhad; Azad, Abul Kalam

2016-09-01

With the aim of providing experimental data on the shear capacity and behavior of corroded reinforced concrete beams that may help in the development of strength prediction models, the test results of 13 corroded and four un-corroded beams are presented. Corrosion damage was induced by accelerated corrosion induction through impressed current. Test results show that loss of shear strength of beams is mostly attributable to two important damage factors namely, the reduction in stirrups area due to corrosion and the corrosion-induced cracking of concrete cover to stirrups. Based on the test data, a method is proposed to predict the residual shear strength of corroded reinforced concrete beams in which residual shear strength is calculated first by using corrosion-reduced steel area alone, and then it is reduced by a proposed reduction factor, which collectively represents all other applicable corrosion damage factors. The method seems to yield results that are in reasonable agreement with the available test data.

Compositions and methods relating to transgenic plants and cellulosic ethanol production

DOEpatents

Tien, Ming [State College, PA; Carlson, John [Port Matilda, PA; Liang, Haiying [Clemson, SC

2012-04-24

Transgenic lignocellulosic plants are provided according to embodiments of the present invention, the transgenic plants transformed with an expression cassette encoding a protein operably linked to a signal peptide which targets the protein to a cell wall of the transgenic plant, where at least 5% of the total amino acid residues of the protein are tyrosine, lysine, serine, threonine or cysteine. Methods of increasing lignin-protein bonds in a lignocellulosic plant are provided according to embodiments of the present invention which include expressing a recombinant nucleic acid in a lignocellulosic plant, the recombinant nucleic acid encoding a protein operably linked to a signal peptide which targets the protein to the cell wall of a plant, where at least 5% of the total amino acid residues of the protein are tyrosine, lysine, serine, threonine or cysteine.

Measurements of 59Ni in meteorites by accelerator mass spectrometry

NASA Astrophysics Data System (ADS)

Paul, M.; Fifield, L. K.; Fink, D.; Albrecht, A.; Allan, G. L.; Herzog, G.; Tuniz, C.

1993-10-01

Isotopic abundances of the radionuclide 59Ni (T1/2 = 76000 yr) were measured by accelerator mass spectrometry with the 14UD Pelletron tandem accelerator at the Australian National University and a detection system solely based on a multianode ionization chamber. The sensitivity limit in the measurement of 59Ni isotopic abundances is 5 × 10-13, as determined by residual interferences from isobaric 59Co and isotopic 58Ni ions. Cosmogenic 59Ni abundances 59Ni/Ni = (8-20) × 10-12 were measured in four samples prepared from the metal phase of two meteorites (mesosiderites). The ratio of the 59Ni abundances to those measured for 41Ca in the silicate phase of the same samples, is in fair agreement with the ratio of the production rates via thermal-neutron capture on 58Ni and 40Ca.

Low concentrations of Al(III) accelerate the formation of biofilm: Multiple effects of hormesis and flocculation.

PubMed

Cui, Xiaochun; Huo, Mingxin; Chen, Congli; Yu, Zhisen; Zhou, Chen; Li, Anran; Qiao, Bingqian; Zhou, Dandan; Crittenden, John C

2018-09-01

Residual Al(III) (at low concentration) is common in water treatment plants (WTPs) and is associated with bacteria. We hypothesize that Al(III) accelerate biofouling due to its hydrolysis and hormesis characteristics, as compared with other cations. To verify this, we elaborated the roles of Al(III) at low concentrations on the biofilm formation. Al(III) hormesis (<2.0mg/L) stimulated bacteria growth increased by ~3.7 times, and extracellular polymeric substances production also enhanced. Al(III) flocculation resulted in the suspended cells precipitation instantly, for Al(III) dosages of 0.6 and 2.0mg/L and the concentration of Al(III) decreased by 0.07 and 0.14mg/L, respectively. Al(III) poisoned the bridged bacterial cells and decreased their ATP by 22.36% and 55.91%, respectively. Al(III) formed polymer presented strong affinity with bacterial outer membrane, and this damaged the bacterial outer membrane. This caused proteins to leak at the combined point. Al-polymer bound to NH 2 and/or NH on the leaked protein, contributed to biofilm formation. Biofilm maturity was aided by polysaccharides, which shielded Al(III) toxicity for the formed biofilm. Thus, the biofilm exhibited a distinguished double-layer microstructure, principally with proteins and inactivated cells at the bottom, polysaccharides and activated cells at the top. Thus, hormesis and flocculation caused by low concentration Al(III) mutually promoted each other, and together accelerated biofilm formation. Copyright © 2018 Elsevier B.V. All rights reserved.

Plant extracts: sense or nonsense?

PubMed

Madersbacher, Stephan; Berger, Ingrid; Ponholzer, Anton; Marszalek, Martin

2008-01-01

To assess the current role of plant extracts in the medical management of lower urinary tract symptoms due to benign prostatic enlargement/benign prostatic obstruction. In 2006, two clinical trials meeting the WHO benign prostatic hyperplasia consensus conference criteria (randomized against placebo/standard therapy, study duration 12 months) were published. One trial compared a saw palmetto extract with placebo. This industry-independent trial published in the New England Journal of Medicine was negative, that is, this saw palmetto extract had no effect on symptoms, Qmax and postvoid residual volume. In another trial, a saw palmetto/urtica combination was compared with tamsulosin. After 12 months, the improvement of symptoms was identical in both study arms. No detailed data were presented, however, on Qmax, postvoid residual or prostate volume. The biological mechanisms of plant extracts in vivo are still unknown and the numerous metaanalyses cannot supplement high-quality prospective trials. Further prospective studies according to WHO benign prostatic hyperplasia standards are required to reliably determine the role of plant extracts in contemporary lower urinary tract symptoms management and to be able to answer the question in the title: 'plant extracts: sense or nonsense?' Plant extracts are currently not recommended by the American and European Association of Urology benign prostatic hyperplasia guidelines.

Structure-function relationship of a plant NCS1 member--homology modeling and mutagenesis identified residues critical for substrate specificity of PLUTO, a nucleobase transporter from Arabidopsis.

PubMed

Witz, Sandra; Panwar, Pankaj; Schober, Markus; Deppe, Johannes; Pasha, Farhan Ahmad; Lemieux, M Joanne; Möhlmann, Torsten

2014-01-01

Plastidic uracil salvage is essential for plant growth and development. So far, PLUTO, the plastidic nucleobase transporter from Arabidopsis thaliana is the only known uracil importer at the inner plastidic membrane which represents the permeability barrier of this organelle. We present the first homology model of PLUTO, the sole plant NCS1 member from Arabidopsis based on the crystal structure of the benzyl hydantoin transporter MHP1 from Microbacterium liquefaciens and validated by molecular dynamics simulations. Polar side chains of residues Glu-227 and backbones of Val-145, Gly-147 and Thr-425 are proposed to form the binding site for the three PLUTO substrates uracil, adenine and guanine. Mutational analysis and competition studies identified Glu-227 as an important residue for uracil and to a lesser extent for guanine transport. A differential response in substrate transport was apparent with PLUTO double mutants E227Q G147Q and E227Q T425A, both of which most strongly affected adenine transport, and in V145A G147Q, which markedly affected guanine transport. These differences could be explained by docking studies, showing that uracil and guanine exhibit a similar binding mode whereas adenine binds deep into the catalytic pocket of PLUTO. Furthermore, competition studies confirmed these results. The present study defines the molecular determinants for PLUTO substrate binding and demonstrates key differences in structure-function relations between PLUTO and other NCS1 family members.

Case study of an MBT plant producing SRF for cement kiln co-combustion, coupled with a bioreactor landfill for process residues.

PubMed

Grosso, Mario; Dellavedova, Stefano; Rigamonti, Lucia; Scotti, Sergio

2016-01-01

The paper describes the performances of the energy recovery pathway from the residual waste based on the production of a Solid Recovered Fuel (SRF) to be exploited via co-combustion in a cement kiln. The SRF is produced in a single stream Mechanical-Biological Treatment plant, where bio-drying of the waste is followed by mechanical refining in order to fulfil the quality requirements by the cement kilns. Peculiar of this MBT is the fact that sorting residues are disposed in a nearby landfill, managed according to a bioreactor approach, where landfill gas is collected for electric energy recovery. A detailed mass and energy balance of the system is presented based on one year operational data, followed by its Life Cycle Assessment. Results show that the system is energetically and environmentally effective, with most of the impacts being more than compensated by the savings of materials and energy. Major role in determining such outcome is the displacement of petcoke in the cement kiln, both in terms of its fossil CO2 emissions and of its life cycle impacts, including the trans-oceanic transport. To check the robustness of the results, two sensitivity analyses are performed on the landfill gas collection efficiency and on the avoided electric energy mix. Copyright © 2015 Elsevier Ltd. All rights reserved.

Neonicotinoid Insecticide Residues in Surface Water and Soil Associated with Commercial Maize (Corn) Fields in Southwestern Ontario

PubMed Central

Schaafsma, Arthur; Limay-Rios, Victor; Baute, Tracey; Smith, Jocelyn; Xue, Yingen

2015-01-01

Neonicotinoid insecticides have come under scrutiny for their potential unintended effects on non-target organisms, particularly pollinators in agro-ecosystems. As part of a larger study of neonicotinoid residues associated with maize (corn) production, 76 water samples within or around the perimeter of 18 commercial maize fields and neighbouring apiaries were collected in 5 maize-producing counties of southwestern Ontario. Residues of clothianidin (mean = 2.28, max. = 43.60 ng/mL) and thiamethoxam (mean = 1.12, max. = 16.50 ng/mL) were detected in 100 and 98.7% of the water samples tested, respectively. The concentration of total neonicotinoid residues in water within maize fields increased six-fold during the first five weeks after planting, and returned to pre-plant levels seven weeks after planting. However, concentrations in water sampled from outside the fields were similar throughout the sampling period. Soil samples from the top 5 cm of the soil profile were also collected in these fields before and immediately following planting. The mean total neonicotinoid residue was 4.02 (range 0.07 to 20.30) ng/g, for samples taken before planting, and 9.94 (range 0.53 to 38.98) ng/g, for those taken immediately after planting. Two soil samples collected from within an conservation area contained detectable (0.03 and 0.11 ng/g) concentrations of clothianidin. Of three drifted snow samples taken, the drift stratum containing the most wind-scoured soil had 0.16 and 0.20 ng/mL mainly clothianidin in the melted snow. The concentration was at the limit of detection (0.02 ng/mL) taken across the entire vertical profile. With the exception of one sample, water samples tested had concentrations below those reported to have acute, chronic or sublethal effects to honey bees. Our results suggest that neonicotinoids may move off-target by wind erosion of contaminated soil. These results are informative to risk assessment models for other non-target species in maize agro

Residues and risks of veterinary antibiotics in protected vegetable soils following application of different manures.

PubMed

Zhang, Haibo; Zhou, Yang; Huang, Yujuan; Wu, Longhua; Liu, Xinghua; Luo, Yongming

2016-06-01

The protected vegetable farming is a style of high frequent rotation farming which requires a huge amount of fertilizers to maintain soil fertility. A total of 125 surface soils covering from east to west of China were sampled for the analysis of 17 antibiotics in order to identify antibiotics contamination caused by long-term manures application. The results indicate that the agricultural land has accumulated a statistically significantly higher antibiotics concentration than conventional open croplands. The maximum oxytetracycline concentration was 8400 μg kg(-1), the highest level that has ever been reported for oxytetracycline in soils. The residual concentration is decided by both plant duration and manure type. Short-term (<5 years) planting shows the highest residues of tetracyclines and fluoroquinolones in the soils. The organic farming characteristic of applying commercial compost as a single fertilizer in planting shows the lowest antibiotics residue in the soils on the whole. Principal component analysis suggests that the various combinations of antibiotic compounds in the soil may be used to trace the manure source. The antibiotics in soil may threaten water quality through contamination by diffusion. Ciprofloxacin and sulfachinoxalin are calculated to be a higher migration risk to surface waters, hence their environmental fate requires further study. Copyright © 2016 Elsevier Ltd. All rights reserved.

[Development of Plant Metabolomics and Medicinal Plant Genomics].

PubMed

Saito, Kazuki

2018-01-01

 A variety of chemicals produced by plants, often referred to as 'phytochemicals', have been used as medicines, food, fuels and industrial raw materials. Recent advances in the study of genomics and metabolomics in plant science have accelerated our understanding of the mechanisms, regulation and evolution of the biosynthesis of specialized plant products. We can now address such questions as how the metabolomic diversity of plants is originated at the levels of genome, and how we should apply this knowledge to drug discovery, industry and agriculture. Our research group has focused on metabolomics-based functional genomics over the last 15 years and we have developed a new research area called 'Phytochemical Genomics'. In this review, the development of a research platform for plant metabolomics is discussed first, to provide a better understanding of the chemical diversity of plants. Then, representative applications of metabolomics to functional genomics in a model plant, Arabidopsis thaliana, are described. The extension of integrated multi-omics analyses to non-model specialized plants, e.g., medicinal plants, is presented, including the identification of novel genes, metabolites and networks for the biosynthesis of flavonoids, alkaloids, sulfur-containing metabolites and terpenoids. Further, functional genomics studies on a variety of medicinal plants is presented. I also discuss future trends in pharmacognosy and related sciences.

Accelerating Planted Green Ash Establishment on an Abandoned Soybean Field

Treesearch

John W. Groninger; Didier A. Babassana

2002-01-01

Planted green ash seedlings exhibit high survival rates on most bottomland sites that have recently come out of row crop production, making this species a popular choice for afforestation. Sub-optimal growth of planted hardwood tree species, including green ash, often delays the realization of many of the economic and environmental benefits that are used to justify the...

Accelerator radiocarbon dating of evidence for prehistoric horticulture in Illinois

USGS Publications Warehouse

Conard, N.; Asch, D.L.; Asch, N.B.; Elmore, D.; Gove, H.; Rubin, M.; Brown, J.A.; Wiant, M.D.; Farnsworth, K.B.; Cook, T.G.

1984-01-01

With the development of direct detection radiocarbon dating, which uses an accelerator as part of a highly selective mass spectrometer, it is now possible to determine the age of milligram samples of organic materials1-5. One application of accelerator dating is in evaluating scanty, sometimes controversial evidence for early horticulture throughout the world. We have now used the technique to date small samples of carbonized, cultivated plant remains from archaeological sites in Illinois. The results, reported here, establish (1) that squash was introduced by 7,000 yr ago, 2,500 yr before eastern North American records previously reported; (2) that horticulture involving indigenous plants had begun by 4,000 BP in eastern North America with domestication of Iva annua, a small-seeded annual; (3) that anomalous discoveries of Archaic period maize represent contaminants; and (4) that introduction of maize by initial Middle Woodland times (~2,000 BP) is questionable.

Espresso coffee residues as a nitrogen amendment for small-scale vegetable production.

PubMed

Cruz, Soraia; Marques dos Santos Cordovil, Cláudia S C

2015-12-01

Espresso coffee grounds constitute a residue which is produced daily in considerable amounts, and is often pointed out as being potentially interesting for plant nutrition. Two experiments (incubations and field experiments) were carried out to evaluate the potential nitrogen (N) and phosphorus (P) supply for carrot (Daucus carota L.), spinach (Spinacea oleracea L.) and lettuce (Lactuca sativa L.) nutrition. Immobilisation of nitrogen and phosphorus was detected in all the incubations and, in the field experiments, germination and yield growth were decreased by the presence of espresso coffee grounds, in general for all the species studied. The study showed an inhibition of N and P mineralisation and a reduction of plant germination and growth. Further research is required to determine whether this is related to the immobilising capacity of the residue or possibly due to the presence of caffeine. © 2015 Society of Chemical Industry.

Life cycle assessment of post-consumer plastics production from waste electrical and electronic equipment (WEEE) treatment residues in a Central European plastics recycling plant.

PubMed

Wäger, Patrick A; Hischier, Roland

2015-10-01

Plastics play an increasingly important role in reaching the recovery and recycling rates defined in the European WEEE Directive. In a recent study we have determined the life cycle environmental impacts of post-consumer plastics production from mixed, plastics-rich WEEE treatment residues in the Central European plant of a market-leading plastics recycler, both from the perspective of the customers delivering the residues and the customers buying the obtained post-consumer recycled plastics. The results of our life cycle assessments, which were extensively tested with sensitivity analyses, show that from both perspectives plastics recycling is clearly superior to the alternatives considered in this study (i.e. municipal solid waste incineration (MSWI) and virgin plastics production). For the three ReCiPe endpoint damage categories, incineration in an MSWI plant results in an impact exceeding that of the examined plastics recycling facility each by about a factor of 4, and the production of virgin plastics has an impact exceeding that of the post-consumer recycled (PCR) plastics production each by a factor of 6-10. On a midpoint indicator level the picture is more differentiated, showing that the environmental impacts of the recycling options are lower by 50% and more for almost all impact factors. While this provides the necessary evidence for the environmental benefits of plastics recycling compared to existing alternatives, it can, however, not be taken as conclusive evidence. To be conclusive, future research will have to address the fate of hazardous substances in the outputs of such recycling systems in more detail. Copyright © 2015 Elsevier B.V. All rights reserved.

Characterization of CTX-M enzymes, quinolone resistance determinants, and antimicrobial residues from hospital sewage, wastewater treatment plant, and river water.

PubMed

Conte, Danieli; Palmeiro, Jussara Kasuko; da Silva Nogueira, Keite; de Lima, Thiago Marenda Rosa; Cardoso, Marco André; Pontarolo, Roberto; Degaut Pontes, Flávia Lada; Dalla-Costa, Libera Maria

2017-02-01

Multidrug-resistant (MDR) bacteria are widespread in hospitals and have been increasingly isolated from aquatic environments. The aim of the present study was to characterize extended-spectrum β-lactamase (ESBL) and quinolone-resistant Enterobacteriaceae from a hospital effluent, sanitary effluent, inflow sewage, aeration tank, and outflow sewage within a wastewater treatment plant (WWTP), as well as river water upstream and downstream (URW and DRW, respectively), of the point where the WWTP treated effluent was discharged. β-lactamase (bla) genes, plasmid-mediated quinolone resistance (PMQR), and quinolone resistance-determining regions (QRDRs) were assessed by amplification and sequencing in 55 ESBL-positive and/or quinolone-resistant isolates. Ciprofloxacin residue was evaluated by high performance liquid chromatography. ESBL-producing isolates were identified in both raw (n=29) and treated (n=26) water; they included Escherichia coli (32), Klebsiella pneumoniae (22) and Klebsiella oxytoca (1). Resistance to both cephalosporins and quinolone was observed in 34.4% of E. coli and 27.3% of K. pneumoniae. Resistance to carbapenems was found in 5.4% of K. pneumoniae and in K. oxytoca. Results indicate the presence of bla CTX-M (51/55, 92.7%) and bla SHV (8/55, 14.5%) ESBLs, and bla GES (2/55, 3.6%) carbapenemase-encoding resistance determinants. Genes conferring quinolone resistance were detected at all sites, except in the inflow sewage and aeration tanks. Quinolone resistance was primarily attributed to amino acid substitutions in the QRDR of GyrA (47%) or to the presence of PMQR (aac-(6')-Ib-cr, oqxAB, qnrS, and/or qnrB; 52.9%) determinants. Ciprofloxacin residue was absent only from URW. Our results have shown strains carrying ESBL genes, PMQR determinants, and mutations in the gyrA QRDR genes mainly in hospital effluent, URW, and DRW samples. Antimicrobial use, and the inefficient removal of MDR bacteria and antibiotic residue during sewage treatment, may

Measurement of Residual Flexibility for Substructures Having Prominent Flexible Interfaces

NASA Technical Reports Server (NTRS)

Tinker, Michael L.; Bookout, Paul S.

1994-01-01

structure in both test and analysis. Measured and predicted residual functions are compared, and regions of poor data in the measured curves are described. It is found that for accurate residual measurements, frequency response functions having prominent stiffness lines in the acceleration/force format are needed. The lack of such stiffness lines increases measurement errors. Interface drive point frequency respose functions for shuttle orbiter payloads exhibit dominant stiffness lines, making the residual test approach a good candidate for payload modal tests when constrained tests are inappropriate. Difficulties in extracting a residual flexibility value from noisy test data are discussed. It is shown that use of a weighted second order least-squares curve fit of the measured residual function allows identification of residual flexibility that compares very well with predictions for the simple structure. This approach also provides an estimate of second order residual mass effects.

Compositions and methods relating to transgenic plants and cellulosic ethanol production

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tien, Ming; Carlson, John; Liang, Haiying

Transgenic lignocellulosic plants are provided according to embodiments of the present invention, the transgenic plants transformed with an expression cassette encoding a protein operably linked to a signal peptide which targets the protein to a cell wall of the transgenic plant, where at least 5% of the total amino acid residues of the protein are tyrosine, lysine, serine, threonine or cysteine. Methods of increasing lignin-protein bonds in a lignocellulosic plant are provided according to embodiments of the present invention which include expressing a recombinant nucleic acid in a lignocellulosic plant, the recombinant nucleic acid encoding a protein operably linked tomore » a signal peptide which targets the protein to the cell wall of a plant, where at least 5% of the total amino acid residues of the protein are tyrosine, lysine, serine, threonine or cysteine.« less

Fast and high-efficiency magnetic surface imprinting based on microwave-accelerated reversible addition fragmentation chain transfer polymerization for the selective extraction of estrogen residues in milk.

PubMed

Chen, Fangfang; Wang, Jiayu; Lu, Ruicong; Chen, Huiru; Xie, Xiaoyu

2018-08-10

A novel microwave-accelerated reversible addition fragmentation chain transfer (RAFT) polymerization strategy has been introduced to shorten reaction time and improved polymerization efficiency of the conventional molecularly imprinting technology based on RAFT. Magnetic molecular imprinted polymers (MMIPs) were successfully synthesized much more efficiently using 17β-estradiol (E2) as a template for the determination of estrogen residues. The resultant MMIPs had well-defined thin imprinted film, favoring the fast mass transfer. Moreover, the reaction time, which was just 1/24 of the time taken by conventional heating, was significantly decreased, improving the reaction efficiency and reducing the probability of side reactions. Meanwhile, the obtained polymers have good capacity of 6.67 mg g -1 and satisfactory selectivity to template molecule with the imprinting factor of 5.11. As a result, a method combination of the resultant MMIPs as solid phase extraction sorbents and high-performance liquid chromatography was successfully set up to determinate three estrogen residues in milk samples. For E2, estrone, and estriol, the limit of detections were calculated to be 0.03, 0.08, and 0.06 ng mL -1 , respectively, and the limit of quantifications were 0.11, 0.27, and 0.21 ng mL -1 , respectively. At the spiked level of 1, 5, and 10 ng mL -1 , the recoveries of the three estrogens were ranged from 69.1% to 91.9% and the intra-day relative standard deviation (RSD) was less than 5.7%. In addition, the resultant MMIPs exhibited good reproducibility and reusability with the inter-batch RSD of 5.3% and the intra-batch RSD of 6.2%, respectively. Overall, the realization of this strategy facilitates the preparation of MMIPs with good architecture and high reaction efficiencies for the analysis of complicated real samples. Copyright © 2018 Elsevier B.V. All rights reserved.

Characterization of Coal Combustion Residues from Electric Utilities--Leaching and Characterization Data

EPA Science Inventory

This report evaluates changes in composition and constituent release by leaching that may occur to fly ash and other coal combustion residues (CCRs) in response to changes in air pollution control technology at coal-fired power plants. The addition of flue-gas desulfurization (FG...

Protecting Accelerator Control Systems in the Face of Sophisticated Cyber Attacks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hartman, Steven M

2012-01-01

Cyber security for industrial control systems has received significant attention in the past two years. The news coverage of the Stuxnet attack, believed to be targeted at the control system for a uranium enrichment plant, brought the issue to the attention of news media and policy makers. This has led to increased scrutiny of control systems for critical infrastructure such as power generation and distribution, and industrial systems such as chemical plants and petroleum refineries. The past two years have also seen targeted network attacks aimed at corporate and government entities including US Department of Energy National Laboratories. Both ofmore » these developments have potential repercussions for the control systems of particle accelerators. The need to balance risks from potential attacks with the operational needs of an accelerator present a unique challenge for the system architecture and access model.« less

Integrating biological treatment of crop residue into a hydroponic sweetpotato culture

NASA Astrophysics Data System (ADS)

Trotman, A. A.; David, P. P.; Bonsi, C. K.; Hill, W. A.; Mortley, D. G.; Loretan, P. A.

1997-01-01

Residual biomass from hydroponic culture of sweetpotato [Ipomoea batatas (L.) Lam.] was degraded using natural bacterial soil isolates. Sweetpotato was grown for 120 days in hydroponic culture with a nutrient solution comprised of a ratio of 80% modified half Hoagland solution to 20% filtered effluent from an aerobic starch hydrolysis bioreactor. The phytotoxicity of the effluent was assayed with `Waldmann's Green' lettuce (Lactuca sativa L.) and the ratio selected after a 60-day bioassay using sweetpotato plants propagated vegetatively from cuttings. Controlled environment chamber experiments were conducted to investigate the impact of filtrate from biological treatment of crop residue on growth and storage root production with plants grown in a modified half Hoagland solution. Incorporation of bioreactor effluent, reduced storage root yield of `Georgia Jet' sweetpotato but the decrease was not statistically significant when compared with yield for plants cultured in a modified half Hoagland solution without filtrate. However, yield of `TU-82-155' sweetpotato was significantly reduced when grown in a modified half Hoagland solution into which filtered effluent had been incorporated. Total biomass was significantly reduced for both sweetpotato cultivars when grown in bioreactor effluent. The leaf area and dry matter accumulation were significantly (P < 0.05) reduced for both cultivars when grown in solution culture containing 20% filtered effluent.

Hydroponic potato production on nutrients derived from anaerobically-processed potato plant residues

NASA Astrophysics Data System (ADS)

Mackowiak, C. L.; Stutte, G. W.; Garland, J. L.; Finger, B. W.; Ruffe, L. M.

1997-01-01

Bioregenerative methods are being developed for recycling plant minerals from harvested inedible biomass as part of NASA's Advanced Life Support (ALS) research. Anaerobic processing produces secondary metabolites, a food source for yeast production, while providing a source of water soluble nutrients for plant growth. Since NH_4-N is the nitrogen product, processing the effluent through a nitrification reactor was used to convert this to NO_3-N, a more acceptable form for plants. Potato (Solanum tuberosum L.) cv. Norland plants were used to test the effects of anaerobically-produced effluent after processing through a yeast reactor or nitrification reactor. These treatments were compared to a mixed-N treatment (75:25, NO_3:NH_4) or a NO_3-N control, both containing only reagent-grade salts. Plant growth and tuber yields were greatest in the NO_3-N control and yeast reactor effluent treatments, which is noteworthy, considering the yeast reactor treatment had high organic loading in the nutrient solution and concomitant microbial activity.

Partitioning Residue-derived and Residue-induced Emissions of N2O Using 15N-labelled Crop Residues

NASA Astrophysics Data System (ADS)

Farrell, R. E.; Carverhill, J.; Lemke, R.; Knight, J. D.

2014-12-01

Estimates of N2O emissions in Canada indicate that 17% of all agriculture-based emissions are associated with the decomposition of crop residues. However, research specific to the western Canadian prairies (including Saskatchewan) has shown that the N2O emission factor for N sources in this region typically ranges between 0.2 and 0.6%, which is well below the current IPCC default emission factor of 1.0%. Thus, it stands to reason that emissions from crop residues should also be lower than those calculated using the current IPCC emission factor. Current data indicates that residue decomposition, N mineralization and N2O production are affected by a number of factors such as C:N ratio and chemical composition of the residue, soil type, and soil water content; thus, a bench-scale incubation study was conducted to examine the effects of soil type and water content on N2O emissions associated with the decomposition of different crop residues. The study was carried out using soils from the Black, Dark Brown, Brown, and Gray soil zones and was conducted at both 50% and 70% water-filled pore space (WFPS); the soils were amended with 15N-labeled residues of wheat, pea, canola, and flax, or with an equivalent amount of 15N-labeled urea; 15N2O production was monitored using a Picarro G5101-i isotopic N2O analyzer. Crop residue additions to the soils resulted in both direct and indirect emissions of N2O, with residue derived emissions (RDE; measured as 15N2O) generally exceeding residue-induced emissions (RIE) at 50% WFPS—with RDEs ranging from 42% to 88% (mean = 58%) of the total N2O. Conversely, at 70% WFPS, RDEs were generally lower than RIEs—ranging from 21% to 83% (mean = 48%). Whereas both water content and soil type had an impact on N2O production, there was a clear and consistent trend in the emission factors for the residues; i.e., emissions were always greatest for the canola residue and lowest for the wheat residue and urea fertilizer; and intermediate for pea

A Truncated Nuclear Norm Regularization Method Based on Weighted Residual Error for Matrix Completion.

PubMed

Qing Liu; Zhihui Lai; Zongwei Zhou; Fangjun Kuang; Zhong Jin

2016-01-01

Low-rank matrix completion aims to recover a matrix from a small subset of its entries and has received much attention in the field of computer vision. Most existing methods formulate the task as a low-rank matrix approximation problem. A truncated nuclear norm has recently been proposed as a better approximation to the rank of matrix than a nuclear norm. The corresponding optimization method, truncated nuclear norm regularization (TNNR), converges better than the nuclear norm minimization-based methods. However, it is not robust to the number of subtracted singular values and requires a large number of iterations to converge. In this paper, a TNNR method based on weighted residual error (TNNR-WRE) for matrix completion and its extension model (ETNNR-WRE) are proposed. TNNR-WRE assigns different weights to the rows of the residual error matrix in an augmented Lagrange function to accelerate the convergence of the TNNR method. The ETNNR-WRE is much more robust to the number of subtracted singular values than the TNNR-WRE, TNNR alternating direction method of multipliers, and TNNR accelerated proximal gradient with Line search methods. Experimental results using both synthetic and real visual data sets show that the proposed TNNR-WRE and ETNNR-WRE methods perform better than TNNR and Iteratively Reweighted Nuclear Norm (IRNN) methods.

Hydroponic Crop Production using Recycled Nutrients from Inedible Crop Residues

NASA Technical Reports Server (NTRS)

Garland, Jay L.; Mackowiak, Cheryl L.; Sager, John C.

1993-01-01

The coupling of plant growth and waste recycling systems is an important step toward the development of bioregenerative life support systems. This research examined the effectiveness of two alternative methods for recycling nutrients from the inedible fraction (residue) of candidate crops in a bioregenerative system as follows: (1) extraction in water, or leaching, and (2) combustion at 550 C, with subsequent reconstitution of the ash in acid. The effectiveness of the different methods was evaluated by (1) comparing the percent recovery of nutrients, and (2) measuring short- and long-term plant growth in hydroponic solutions, based on recycled nutrients.

Effects of thinning, residue mastication, and prescribed fire on soil and nutrient budgets in a Sierra Nevada mixed-conifer forest

USDA-ARS?s Scientific Manuscript database

The effects of thinning followed by residue mastication (THIN), prescribed fire (BURN), and thinning plus residue mastication plus burning (T+B) on nutrient budgets and resin-based (plant root simulator [PRS] probe) measurements of soil nutrient availability in a mixed-conifer forest were measured. ...

Effects of thinning, residue mastication, and prescribed fire on soil and nutrient budgets in a Sierra Nevada mixed conifer forest

USDA-ARS?s Scientific Manuscript database

The effects of thinning followed by residue mastication (THIN), prescribed fire (BURN), and thinning plus residue mastication plus burning (T+B) on nutrient budgets and resin-based (plant root simulator [PRS] probe) measurements of soil nutrient availability in a mixed-conifer forest were measured. ...

Thermal treatment of solid residues from WtE units: a review.

PubMed

Lindberg, Daniel; Molin, Camilla; Hupa, Mikko

2015-03-01

Thermal treatment methods of bottom ash, fly ash and various types of APC (air pollution control) residues from waste-to-energy plants can be used to obtain environmentally stable material. The thermal treatment processes are meant to reduce the leachability of harmful residue constituents, destroy toxic organic compounds, reduce residue volume, and produce material suitable for utilization. Fly ash and APC residues often have high levels of soluble salts, particularly chlorides, metals such as cadmium, lead, copper and zinc, and trace levels of organic pollutants such as dioxins and furans. Different thermal treatment methods can be used to either decompose or stabilize harmful elements and compounds in the ash, or separate them from the ash to get a material that can be safely stored or used as products or raw materials. In the present paper, thermal treatment methods, such as sintering, vitrification, and melting have been reviewed. In addition to a review of the scientific literature, a survey has been made of the extensive patent literature in the field. Copyright © 2015 Elsevier Ltd. All rights reserved.

UNDERSTANDING PLANT-SOIL RELATIONSHIPS USING CONTROLLED ENVIRONMENT FACILITIES

EPA Science Inventory

Although soil is a component of terrestrial ecosystems, it is comprised of a complex web of interacting organisms, and therefore, can be considered itself as an ecosystem. Soil microflora and fauna derive energy from plants and plant residues and serve important functions in mai...

Selective Loss of Cysteine Residues and Disulphide Bonds in a Potato Proteinase Inhibitor II Family

PubMed Central

Li, Xiu-Qing; Zhang, Tieling; Donnelly, Danielle

2011-01-01

Disulphide bonds between cysteine residues in proteins play a key role in protein folding, stability, and function. Loss of a disulphide bond is often associated with functional differentiation of the protein. The evolution of disulphide bonds is still actively debated; analysis of naturally occurring variants can promote understanding of the protein evolutionary process. One of the disulphide bond-containing protein families is the potato proteinase inhibitor II (PI-II, or Pin2, for short) superfamily, which is found in most solanaceous plants and participates in plant development, stress response, and defence. Each PI-II domain contains eight cysteine residues (8C), and two similar PI-II domains form a functional protein that has eight disulphide bonds and two non-identical reaction centres. It is still unclear which patterns and processes affect cysteine residue loss in PI-II. Through cDNA sequencing and data mining, we found six natural variants missing cysteine residues involved in one or two disulphide bonds at the first reaction centre. We named these variants Pi7C and Pi6C for the proteins missing one or two pairs of cysteine residues, respectively. This PI-II-7C/6C family was found exclusively in potato. The missing cysteine residues were in bonding pairs but distant from one another at the nucleotide/protein sequence level. The non-synonymous/synonymous substitution (Ka/Ks) ratio analysis suggested a positive evolutionary gene selection for Pi6C and various Pi7C. The selective deletion of the first reaction centre cysteine residues that are structure-level-paired but sequence-level-distant in PI-II illustrates the flexibility of PI-II domains and suggests the functionality of their transient gene versions during evolution. PMID:21494600

Structure-Function Relationship of a Plant NCS1 Member – Homology Modeling and Mutagenesis Identified Residues Critical for Substrate Specificity of PLUTO, a Nucleobase Transporter from Arabidopsis

PubMed Central

Witz, Sandra; Panwar, Pankaj; Schober, Markus; Deppe, Johannes; Pasha, Farhan Ahmad; Lemieux, M. Joanne; Möhlmann, Torsten

2014-01-01

Plastidic uracil salvage is essential for plant growth and development. So far, PLUTO, the plastidic nucleobase transporter from Arabidopsis thaliana is the only known uracil importer at the inner plastidic membrane which represents the permeability barrier of this organelle. We present the first homology model of PLUTO, the sole plant NCS1 member from Arabidopsis based on the crystal structure of the benzyl hydantoin transporter MHP1 from Microbacterium liquefaciens and validated by molecular dynamics simulations. Polar side chains of residues Glu-227 and backbones of Val-145, Gly-147 and Thr-425 are proposed to form the binding site for the three PLUTO substrates uracil, adenine and guanine. Mutational analysis and competition studies identified Glu-227 as an important residue for uracil and to a lesser extent for guanine transport. A differential response in substrate transport was apparent with PLUTO double mutants E227Q G147Q and E227Q T425A, both of which most strongly affected adenine transport, and in V145A G147Q, which markedly affected guanine transport. These differences could be explained by docking studies, showing that uracil and guanine exhibit a similar binding mode whereas adenine binds deep into the catalytic pocket of PLUTO. Furthermore, competition studies confirmed these results. The present study defines the molecular determinants for PLUTO substrate binding and demonstrates key differences in structure-function relations between PLUTO and other NCS1 family members. PMID:24621654

Hycrest crested wheatgrass accelerates the degradation of pentachlorophenol in soil

NASA Technical Reports Server (NTRS)

Ferro, A. M.; Sims, R. C.; Bugbee, B.

1994-01-01

We investigated the effects of vegetation on the fate of pentachlorophenol (PCP) in soil using a novel high-flow sealed test system. Pentachlorophenol has been widely used as a wood preservative, and this highly toxic biocide contaminates soil and ground water at many sites. Although plants are known to accelerate the rates of degradation of certain soil contaminants, this approach has not been thoroughly investigated for PCP. The fate of [14C]PCP, added to soil at a concentration of 100 mg/kg, was compared in three unplanted and three planted systems. The plant used was Hycrest, a perennial, drought-tolerant cultivar of crested wheatgrass [Agropyron desertorum (Fischer ex Link) Schultes]. The flow-through test system allowed us to maintain a budget for 14C-label as well as monitor mineralization (breakdown to 14CO2) and volatilization of the test compound in a 155-d trial. In the unplanted systems, an average of 88% of the total radiolabel remained in the soil and leachate and only 6% was mineralized. In the planted system, 33% of the radiolabel remained in the soil plus leachate, 22% was mineralized, and 36% was associated with plant tissue (21% with the root fraction and 15% with shoots). Mineralization rates were 23.1 mg PCP mineralized kg-1 soil in 20 wk in the planted system, and for the unplanted system 6.6 mg PCP kg-1 soil for the same time period. Similar amounts of volatile organic material were generated in the two systems (1.5%). Results indicated that establishing crested wheatgrass on PCP-contaminated surface soils may accelerate the removal of the contaminant.

Contributions of residue-C and -N to plant growth and soil organic matter pools under planted and unplanted conditions

USDA-ARS?s Scientific Manuscript database

Soil microorganisms are considered the most effective decomposers of applied crop residues, but it is poorly understood which communities are primarily responsible for decomposition under different conditions. A pot experiment was conducted in a greenhouse to follow the cycling of C and N derived fr...

Safe apples for baby-food production: survey of pesticide treatment regimes leaving minimum residues.

PubMed

Ticha, Jana; Hajslova, Jana; Kovalczuk, Tomas; Jech, Martin; Honzicek, Jiri; Kocourek, Vladimir; Lansky, Miroslav; Kloutvorova, Jana; Falta, Vladan

2007-06-01

A total of 19 pesticide preparations were used according to agricultural practice in six trials in apple orchards. Using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), premature Golden Delicious apples collected 64, 50, 36 days before harvest and mature fruit were examined for residues of active ingredients. No residues of triflumuron, triazamate, chlorpyrifos, etofenprox, fenoxycarb, kresoxim-methyl, cyprodinyl, difenoconazole or thiram were detected in the first sampling. Also, the levels of chlorpyrifos-methyl, penconazole, tebuconazole and tolylfluanid dropped during the pre-harvest interval. Detectable residues of pyridaben, thiacloprid, trifloxystrobin and tetraconazole in harvested fruits were below 0.01 mg kg(-1), which is the maximum concentration of residues acceptable by baby-food producers in any raw material. The only residues exceeding this concentration were captan and teflubenzuron. Based on the data, farmers can choose pesticides for optimal treatment of plants, while enabling growth of a safe crop suitable for baby-food production.

Phytozome Comparative Plant Genomics Portal

DOE Office of Scientific and Technical Information (OSTI.GOV)

Goodstein, David; Batra, Sajeev; Carlson, Joseph

2014-09-09

The Dept. of Energy Joint Genome Institute is a genomics user facility supporting DOE mission science in the areas of Bioenergy, Carbon Cycling, and Biogeochemistry. The Plant Program at the JGI applies genomic, analytical, computational and informatics platforms and methods to: 1. Understand and accelerate the improvement (domestication) of bioenergy crops 2. Characterize and moderate plant response to climate change 3. Use comparative genomics to identify constrained elements and infer gene function 4. Build high quality genomic resource platforms of JGI Plant Flagship genomes for functional and experimental work 5. Expand functional genomic resources for Plant Flagship genomes

Residue level and dissipation of carbendazim in/on pomegranate fruits and soil.

PubMed

Mohapatra, Soudamini; S, Lekha

2016-07-01

Carbendazim is widely used on pomegranate for control of a large number of fungal diseases. Its residue levels in/on pomegranate fruits and soil were evaluated under field conditions. The quick, easy, cheap, effective, rugged, and safe (QuEChERS) method in conjunction with liquid-chromatography mass spectrometry was used for analysis of carbendazim. Recovery of carbendazim was within 78.92-96.28 % and relative standard deviation within 3.8-10.9 % (n = 6). Carbendazim residues on pomegranate fruits dissipated at the half lives of 17.3 and 22.8 days from treatments at 500 and 1000 g active ingredient (a.i.) ha(-1), respectively. Its residues in pomegranate aril were highest on the tenth day and reduced thereafter. The residue level of carbendazim on pomegranate whole fruits from standard dose treatment was less than the EU maximum residue limit (MRL) of 0.1 mg kg(-1) at harvest. The carbendazim residues were plant protection of pomegranate crop.

Long-term shifts in the phenology of rare and endemic Rocky Mountain plants.

PubMed

Munson, Seth M; Sher, Anna A

2015-08-01

• Mountainous regions support high plant productivity, diversity, and endemism, yet are highly vulnerable to climate change. Historical records and model predictions show increasing temperatures across high elevation regions including the Southern Rocky Mountains, which can have a strong influence on the performance and distribution of montane plant species. Rare plant species can be particularly vulnerable to climate change because of their limited abundance and distribution.• We tracked the phenology of rare and endemic species, which are identified as imperiled, across three different habitat types with herbarium records to determine if flowering time has changed over the last century, and if phenological change was related to shifts in climate.• We found that the flowering date of rare species has accelerated 3.1 d every decade (42 d total) since the late 1800s, with plants in sagebrush interbasins showing the strongest accelerations in phenology. High winter temperatures were associated with the acceleration of phenology in low elevation sagebrush and barren river habitats, whereas high spring temperatures explained accelerated phenology in the high elevation alpine habitat. In contrast, high spring temperatures delayed the phenology of plant species in the two low-elevation habitats and precipitation had mixed effects depending on the season.• These results provide evidence for large shifts in the phenology of rare Rocky Mountain plants related to climate, which can have strong effects on plant fitness, the abundance of associated wildlife, and the future of plant conservation in mountainous regions. © 2015 Botanical Society of America, Inc.

Pharmaceutical residues in the drinking water supply: modeling residue concentrations in surface waters of drugs prescribed in the United States.

PubMed

Guerrero-Preston, Rafael; Brandt-Rauf, Paul

2008-09-01

Pharmaceutical residues and other organic wastewater contaminants (OWC) have been shown to survive conventional water-treatment processes and persist in potable water supplies. To estimate the geographical distribution of the Predicted Environmental Concentration (PEC) of selected drugs prescribed by office based physicians in the United States (US), after non-metabolized residues have been excreted and processed in wastewater treatment plants. The geographical distribution of the PEC in surface waters of pharmaceutical residues was calculated, in four regions of the US. Prescription drug data was obtained from the National Ambulatory Medical Care Survey (NAMCS). The PEC of three drugs prescribed by office based physicians in the US between 1998 and 2000 was compared to the concentrations of these pharmaceuticals found in a surface water characterization project conducted by the United States Geological Survey between 1999 and 2000. There were 803,185,420 medications prescribed by office-based physicians in the US between 1998 and 2000. Relief of pain, hormonal, cardiovascular and antimicrobial medications followed very similar prescription patterns, both in terms of quantity and geographical distribution. Together these four types of medications account for more than half of the medications prescribed between 1998 and 2000. The concentration of pharmaceutical residues found in the drinking water supply was not significantly correlated to the PEC of pharmaceuticals prescribed by office-based physicians. The geographical distribution of medications prescribed by office based physicians in the US underlines the need to implement effective public health strategies.

First muon acceleration using a radio-frequency accelerator

NASA Astrophysics Data System (ADS)

Bae, S.; Choi, H.; Choi, S.; Fukao, Y.; Futatsukawa, K.; Hasegawa, K.; Iijima, T.; Iinuma, H.; Ishida, K.; Kawamura, N.; Kim, B.; Kitamura, R.; Ko, H. S.; Kondo, Y.; Li, S.; Mibe, T.; Miyake, Y.; Morishita, T.; Nakazawa, Y.; Otani, M.; Razuvaev, G. P.; Saito, N.; Shimomura, K.; Sue, Y.; Won, E.; Yamazaki, T.

2018-05-01

Muons have been accelerated by using a radio-frequency accelerator for the first time. Negative muonium atoms (Mu- ), which are bound states of positive muons (μ+) and two electrons, are generated from μ+'s through the electron capture process in an aluminum degrader. The generated Mu- 's are initially electrostatically accelerated and injected into a radio-frequency quadrupole linac (RFQ). In the RFQ, the Mu- 's are accelerated to 89 keV. The accelerated Mu- 's are identified by momentum measurement and time of flight. This compact muon linac opens the door to various muon accelerator applications including particle physics measurements and the construction of a transmission muon microscope.

Delayed degradation in soil of foliar herbicides glyphosate and sulcotrione previously absorbed by plants: consequences on herbicide fate and risk assessment.

PubMed

Doublet, Jérémy; Mamy, Laure; Barriuso, Enrique

2009-10-01

Following application, pesticides can be intercepted and absorbed by weeds and/or crops. Plants containing pesticides residues may then reach the soil during the crop cycle or after harvest. However, the fate in soil of pesticides residues in plants is unknown. Two commonly used foliar herbicides, glyphosate and sulcotrione, (14)C-labeled, were applied on leaves of oilseed rape and/or maize, translocation was studied, and then soil incubations of aerial parts of plants containing herbicides residues were performed. Soil treated directly with herbicides was used as control. The effects of adjuvants on herbicide plant-absorption and subsequent soil-degradation were also investigated comparing herbicides application as active ingredients and as commercial formulations. The fate in soil of herbicides residues in plants was different from that of control, and different for glyphosate and sulcotrione. Mineralization in soil of glyphosate in crops decreased compared to control, and amounts of (14)C-extractable residues, mainly composed by the metabolite aminomethylphosphonic acid (AMPA), and non-extractable residues (NER) increased. In contrast, mineralization in soil of sulcotrione in maize increased compared to control, with a decrease in the (14)C-extractable residues and an increase in NER. The fate of both herbicides was influenced by the type of plant organ in which herbicide was incorporated, because of differences in herbicides bioavailability and organs biodegradability, but not by adjuvants. Absorption of both herbicides in plant delays their subsequent soil-degradation, and particularly, glyphosate persistence in soil could increase from two to six times. The modifications of herbicide degradation in soil due to interception by plants should be considered for environmental risks assessment.

Comparative management of offshore posidonia residues: composting vs. energy recovery.

PubMed

Cocozza, Claudio; Parente, Angelo; Zaccone, Claudio; Mininni, Carlo; Santamaria, Pietro; Miano, Teodoro

2011-01-01

Residues of the marine plant posidonia (Posidonia oceanica, PO) beached in tourist zones represent a great environmental, economical, social and hygienic problem in the Mediterranean Basin, in general, and in the Apulia Region in particular, because of the great disturb to the bathers and population, and the high costs that the administrations have to bear for their removal and disposal. In the present paper, Authors determined the heating values of leaves and fibres of PO, the main offshore residues found on beaches, and, meantime, composted those residues with mowing and olive pruning wood. The final composts were characterized for pH, electrical conductivity, elemental composition, dynamic respiration index, phytotoxicity, fluorescence and infrared spectroscopic fingerprints. The aim of the paper was to investigate the composting and energy recovery of PO leaves and fibres in order to suggest alternative solutions to the landfill when offshore residues have to be removed from recreational beaches. The fibrous portion of PO residues showed heating values close to those of other biofuels, thus suggesting a possible utilization as source of energy. At the same time, compost obtained from both PO wastes showed high quality features on condition that the electrical conductivity and Na content are lowered by a correct management of wetting during the composting. Copyright © 2010 Elsevier Ltd. All rights reserved.

New technologies accelerate the exploration of non-coding RNAs in horticultural plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Degao; Mewalal, Ritesh; Hu, Rongbin

Non-coding RNAs (ncRNAs), that is, RNAs not translated into proteins, are crucial regulators of a variety of biological processes in plants. While protein-encoding genes have been relatively well-annotated in sequenced genomes, accounting for a small portion of the genome space in plants, the universe of plant ncRNAs is rapidly expanding. Recent advances in experimental and computational technologies have generated a great momentum for discovery and functional characterization of ncRNAs. Here we summarize the classification and known biological functions of plant ncRNAs, review the application of next-generation sequencing (NGS) technology and ribosome profiling technology to ncRNA discovery in horticultural plants andmore » discuss the application of new technologies, especially the new genome-editing tool clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems, to functional characterization of plant ncRNAs.« less

New technologies accelerate the exploration of non-coding RNAs in horticultural plants

PubMed Central

Liu, Degao; Mewalal, Ritesh; Hu, Rongbin; Tuskan, Gerald A; Yang, Xiaohan

2017-01-01

Non-coding RNAs (ncRNAs), that is, RNAs not translated into proteins, are crucial regulators of a variety of biological processes in plants. While protein-encoding genes have been relatively well-annotated in sequenced genomes, accounting for a small portion of the genome space in plants, the universe of plant ncRNAs is rapidly expanding. Recent advances in experimental and computational technologies have generated a great momentum for discovery and functional characterization of ncRNAs. Here we summarize the classification and known biological functions of plant ncRNAs, review the application of next-generation sequencing (NGS) technology and ribosome profiling technology to ncRNA discovery in horticultural plants and discuss the application of new technologies, especially the new genome-editing tool clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems, to functional characterization of plant ncRNAs. PMID:28698797

Pesticide residues in stone fruits from the south-eastern region of Poland in 2012-2104.

PubMed

Słowik-Borowiec, Magdalena; Szpyrka, Ewa; Rupar, Julian; Matyaszek, Aneta; Podbielska, Magdalena

2015-01-01

Peaches, sour cherries, nectarines, apricots, plums and cherries are fruit commonly known as "stone fruit". Their nutritional properties namely, vitamins, minerals, fiber and numerous microelements, make them a very important component of human diet. As fruit trees can be attacked by numerous diseases and pests, chemical protection of these crops is used. Therefore, it is important that the relevant governmental agencies or institutions ensure correct application of pesticides. The aim of the study was to evaluate the occurrence of pesticide residues in stone fruits south-eastern region of Poland in 2012-2014 in order to provide data to estimate health risk to consumers. Validated analytical methods based on liquid / liquid extraction coupled with gas chromatography with electron capture and nitrogen phosphorus detection (GC-ECD/NPD) and spectrophotometry (dithiocarbamates residues) were used for the analysis. 92 samples of stone fruits were tested for the presence of pesticide residues. 13 of all samples (14%) contained pesticide residues. 7 active substances were detected, including 5 fungicides: boscalide, bupirimate, difenoconazole, dithiocarbamates and captan, and 2 insecticides: cypermethrin and pirimicarb. In the analysed samples, the use of not recommended plant protection products in orchard crops were found. However, neither maximum residue levels (MRLs) recommended by the Regulation (EC) No 396/2005 were exceeded nor pesticides being unapproved by the Regulation (EC) No 1107/2009 detected in the analysed samples. Lack of plant protection products for control specific diseases or pests in crops results in the use of formulations not recommended for use in certain orchard crops. On a basis of results reported in previous years it can be concluded that occurrence of pesticide residues in stone fruit samples dropped significantly.

PARTICLE ACCELERATOR

DOEpatents

Teng, L.C.

1960-01-19

ABS>A combination of two accelerators, a cyclotron and a ring-shaped accelerator which has a portion disposed tangentially to the cyclotron, is described. Means are provided to transfer particles from the cyclotron to the ring accelerator including a magnetic deflector within the cyclotron, a magnetic shield between the ring accelerator and the cyclotron, and a magnetic inflector within the ring accelerator.

Plastid-Nuclear Interaction and Accelerated Coevolution in Plastid Ribosomal Genes in Geraniaceae.

PubMed

Weng, Mao-Lun; Ruhlman, Tracey A; Jansen, Robert K

2016-06-27

Plastids and mitochondria have many protein complexes that include subunits encoded by organelle and nuclear genomes. In animal cells, compensatory evolution between mitochondrial and nuclear-encoded subunits was identified and the high mitochondrial mutation rates were hypothesized to drive compensatory evolution in nuclear genomes. In plant cells, compensatory evolution between plastid and nucleus has rarely been investigated in a phylogenetic framework. To investigate plastid-nuclear coevolution, we focused on plastid ribosomal protein genes that are encoded by plastid and nuclear genomes from 27 Geraniales species. Substitution rates were compared for five sets of genes representing plastid- and nuclear-encoded ribosomal subunit proteins targeted to the cytosol or the plastid as well as nonribosomal protein controls. We found that nonsynonymous substitution rates (dN) and the ratios of nonsynonymous to synonymous substitution rates (ω) were accelerated in both plastid- (CpRP) and nuclear-encoded subunits (NuCpRP) of the plastid ribosome relative to control sequences. Our analyses revealed strong signals of cytonuclear coevolution between plastid- and nuclear-encoded subunits, in which nonsynonymous substitutions in CpRP and NuCpRP tend to occur along the same branches in the Geraniaceae phylogeny. This coevolution pattern cannot be explained by physical interaction between amino acid residues. The forces driving accelerated coevolution varied with cellular compartment of the sequence. Increased ω in CpRP was mainly due to intensified positive selection whereas increased ω in NuCpRP was caused by relaxed purifying selection. In addition, the many indels identified in plastid rRNA genes in Geraniaceae may have contributed to changes in plastid subunits. © The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

Super-Resolution of Plant Disease Images for the Acceleration of Image-based Phenotyping and Vigor Diagnosis in Agriculture.

PubMed

Yamamoto, Kyosuke; Togami, Takashi; Yamaguchi, Norio

2017-11-06

Unmanned aerial vehicles (UAVs or drones) are a very promising branch of technology, and they have been utilized in agriculture-in cooperation with image processing technologies-for phenotyping and vigor diagnosis. One of the problems in the utilization of UAVs for agricultural purposes is the limitation in flight time. It is necessary to fly at a high altitude to capture the maximum number of plants in the limited time available, but this reduces the spatial resolution of the captured images. In this study, we applied a super-resolution method to the low-resolution images of tomato diseases to recover detailed appearances, such as lesions on plant organs. We also conducted disease classification using high-resolution, low-resolution, and super-resolution images to evaluate the effectiveness of super-resolution methods in disease classification. Our results indicated that the super-resolution method outperformed conventional image scaling methods in spatial resolution enhancement of tomato disease images. The results of disease classification showed that the accuracy attained was also better by a large margin with super-resolution images than with low-resolution images. These results indicated that our approach not only recovered the information lost in low-resolution images, but also exerted a beneficial influence on further image analysis. The proposed approach will accelerate image-based phenotyping and vigor diagnosis in the field, because it not only saves time to capture images of a crop in a cultivation field but also secures the accuracy of these images for further analysis.

Changes in soil nitrogen cycling under Norway spruce logging residues on a clear-cut

NASA Astrophysics Data System (ADS)

Smolander, Aino; Lindroos, Antti-Jussi; Kitunen, Veikko

2016-04-01

In Europe, forest biomass is increasingly being used as a source of energy to replace fossil fuels. In practice, this means that logging residues, consisting of green branches and stem tops, are more commonly harvested. In 2012 logging residues were harvested from about one third of clear-cuts in Finland. Our aim was to study how logging residues affect soil organic matter quality, in particular soil N cycling processes and composition of certain groups of plant secondary compounds, tannins and terpenes. Compounds in these groups were of interest because they are abundant in logging residues, and they have been shown to control soil N cycling. In connection with clear-cutting a Norway spruce stand in southern Finland, we established a controlled field experiment by building logging residue piles (40 kg/m2) on study plots. The piles consisted of fresh spruce branches and tops with green foliage. Control plots with no residues were included (0 kg/m2). Changes in soil organic matter properties have now been monitored for three growing seasons. Logging residues affected organic layer properties strongly. For example, they increased net nitrification and nitrate concentrations. There were also increases in the concentrations of certain terpenes and condensed tannins due to the residues. The significance of logging residues on soil processes and properties will be shown.

The effects of forest residual debris disposal on perennial grass emergence, growth, and survival in a ponderosa pine ecotone

Treesearch

Darin J. Law; Peter F. Kolb

2007-01-01

Soil surface conditions can have profound effects on plant seedling emergence and subsequent seedling survival. To test the hypothesis that different soil-surface treatments with logging residue affect range grass seedling emergence and survival, 6 alternative forest-residual treatments were established in the summer of 1998 following thinning of mature trees from...

Competitiveness of biomass-fueled electrical power plants.

Treesearch

Bruce A. McCarl; Darius M. Adams; Ralph J. Alig; John T. Chmelik

2000-01-01

One way countries like the United States can comply with suggested rollbacks in greenhouse gas emissions is by employing power plants fueled with biomass. We examine the competitiveness of biomass-based fuel for electrical power as opposed to coal using a mathematical programming structure. We consider fueling power plants from milling residues, whole trees, logging...

Acceleration Modes and Transitions in Pulsed Plasma Accelerators

NASA Technical Reports Server (NTRS)

Polzin, Kurt A.; Greve, Christine M.

2018-01-01

Pulsed plasma accelerators typically operate by storing energy in a capacitor bank and then discharging this energy through a gas, ionizing and accelerating it through the Lorentz body force. Two plasma accelerator types employing this general scheme have typically been studied: the gas-fed pulsed plasma thruster and the quasi-steady magnetoplasmadynamic (MPD) accelerator. The gas-fed pulsed plasma accelerator is generally represented as a completely transient device discharging in approximately 1-10 microseconds. When the capacitor bank is discharged through the gas, a current sheet forms at the breech of the thruster and propagates forward under a j (current density) by B (magnetic field) body force, entraining propellant it encounters. This process is sometimes referred to as detonation-mode acceleration because the current sheet representation approximates that of a strong shock propagating through the gas. Acceleration of the initial current sheet ceases when either the current sheet reaches the end of the device and is ejected or when the current in the circuit reverses, striking a new current sheet at the breech and depriving the initial sheet of additional acceleration. In the quasi-steady MPD accelerator, the pulse is lengthened to approximately 1 millisecond or longer and maintained at an approximately constant level during discharge. The time over which the transient phenomena experienced during startup typically occur is short relative to the overall discharge time, which is now long enough for the plasma to assume a relatively steady-state configuration. The ionized gas flows through a stationary current channel in a manner that is sometimes referred to as the deflagration-mode of operation. The plasma experiences electromagnetic acceleration as it flows through the current channel towards the exit of the device. A device that had a short pulse length but appeared to operate in a plasma acceleration regime different from the gas-fed pulsed plasma

Accelerated crack growth, residual stress, and a cracked zinc coated pressure shell

NASA Technical Reports Server (NTRS)

Dittman, Daniel L.; Hampton, Roy W.; Nelson, Howard G.

1987-01-01

During a partial inspection of a 42 year old, operating, pressurized wind tunnel at NASA-Ames Research Center, a surface connected defect 114 in. long having an indicated depth of a 0.7 in. was detected. The pressure shell, constructed of a medium carbon steel, contains approximately 10 miles of welds and is cooled by flowing water over its zinc coated external surface. Metallurgical and fractographic analysis showed that the actual detect was 1.7 in. deep, and originated from an area of lack of weld penetration. Crack growth studies were performed on the shell material in the laboratory under various loading rates, hold times, and R-ratios with a simulated shell environment. The combination of zinc, water with electrolyte, and steel formed an electrolytic cell which resulted in an increase in cyclic crack growth rate by as much as 500 times over that observed in air. It was concluded that slow crack growth occurred in the pressure shell by a combination of stress corrosion cracking due to the welding residual stress and corrosion fatigue due to the cyclic operating stress.

Creep Deformation, Rupture Analysis, Heat Treatment and Residual Stress Measurement of Monolithic and Welded Grade 91 Steel for Power Plant Components

NASA Astrophysics Data System (ADS)

Shrestha, Triratna

Modified 9Cr-1 Mo (Grade 91) steel is currently considered as a candidate material for reactor pressure vessels (RPVs) and reactor internals for the Very High Temperature Reactor (VHTR), and in fossil-fuel fired power plants at higher temperatures and stresses. The tensile creep behavior of Grade 91 steel was studied in the temperature range of 600°C to 750°C and stresses between 35 MPa and 350 MPa. Heat treatment of Grade 91 steel was studied by normalizing and tempering the steel at various temperatures and times. Moreover, Thermo-Ca1c(TM) calculation was used to predict the precipitate stability and their evolution, and construct carbon isopleths of Grade 91 steel. Residual stress distribution across gas tungsten arc welds (GTAW) in Grade 91 steel was measured by the time-of-flight neutron diffraction using the Spectrometer for Materials Research at Temperature and Stress (SMARTS) diffractometer at Lujan Neutron Scattering Center, Los Alamos National Laboratory, Los Alamos, NM, USA. Analysis of creep results yielded stress exponents of ˜9-11 in the higher stress regime and ˜1 in the lower stress regime. The creep behavior of Grade 91 steel was described by the modified Bird-Mukherjee-Dorn relation. The rate-controlling creep deformation mechanism in the high stress regime was identified as the edge dislocation climb with a stress exponent of n = 5. On the other hand, the deformation mechanism in the Newtonian viscous creep regime (n = 1) was identified as the Nabarro-Herring creep. Creep rupture data were analyzed in terms of Monkman-Grant relation and Larson-Miller parameter. Creep damage tolerance factor and stress exponent were used to identify the cause of creep damage. The fracture surface morphology of the ruptured specimens was studied by scanning electron microscopy to elucidate the failure mechanisms. Fracture mechanism map for Grade 91 steel was developed based on the available material parameters and experimental observations. The microstructural

[Effect of reduced N application on soil N residue and N loss in maize-soybean relay strip intercropping system].

PubMed

Liu, Xiao-Ming; Yong, Tai-Wen; Liu, Wen-Yu; Su, Ben-Ying; Song, Chun; Yang, Feng; Wang, Xiao-Chun; Yang, Wen-Yu

2014-08-01

A field experiment was conducted in 2012, including three planting pattern (maize-soybean relay strip intercropping, mono-cultured maize and soybean) and three nitrogen application level [0 kg N x hm(-2), 180 kg N x hm(-2) (reduced N) and 240 kg N x hm(-2) (normal N)]. Fields were assigned to different treatments in a randomized block design with three replicates. The objective of this work was to analyze the effects of planting patterns and nitrogen application rates on plant N uptake, soil N residue and N loss. After fertilization applications, NH4(+)-N and NO3(-)-N levels increased in the soil of intercropped maize but decreased in the soil of intercropped soybean. Compared with mono-crops, the soil N residue and loss of intercropped soybean were reduced, while those of intercropped maize were increased and decreased, respectively. With the reduced rate of N application, N residue rate, N loss rate and ammonia volatilization loss rate of the maize-soybean intercropping relay strip system were decreased by 17.7%, 21.5% and 0.4% compared to mono-cultured maize, but increased by 2.0%, 19.8% and 0.1% compared to mono-cultured soybean, respectively. Likewise, the reduced N application resulted in reductions in N residue, N loss, and the N loss via ammonia volatilization in the maize-soybean relay strip intercropping system compared with the conventional rate of N application adopted by local farmers, and the N residue rate, N loss rate and ammonia volatilization loss rate reduced by 12.0%, 15.4% and 1.2%, respectively.

Environmental impacts of anaerobic digestion and the use of anaerobic residues as soil amendment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mosey, F.E.

1996-01-01

This paper defines the environmental role of anaerobic digestion within the overall objective of recovering energy from renewable biomass resources. Examples and opportunities for incorporating anaerobic digestion into biomass-to-energy schemes are discussed, together with environmental aspects of anaerobic digestion plants. These include visual, public amenity, pathogens and public health, odor control, and gaseous emissions. Digestate disposal and the benefits of restrictions on recycling organic wastes and biomass residues back to the land are discussed, particularly as they relate to American and European codes of practice and environmental legislation. The paper concludes that anaerobic digestion, if performed in purpose-designed reactors thatmore » efficiently recover and use biogas, is an environmentally benign process that can enhance energy recovery and aid the beneficial land use of plant residues in many biomass-to-energy schemes.« less

Residual acceleration data on IML-1: Development of a data reduction and dissemination plan

NASA Technical Reports Server (NTRS)

Rogers, Melissa J. B.; Alexander, J. Iwan D.; Wolf, Randy

1992-01-01

The need to record some measure of the low-gravity environment of an orbiting space vehicle was recognized at an early stage of the U.S. Space Program. Such information was considered important for both the assessment of an astronaut's physical condition during and after space missions and the analysis of the fluid physics, materials processing, and biological sciences experiments run in space. Various measurement systems were developed and flown on space platforms beginning in the early 1970's. Similar in concept to land based seismometers that measure vibrations caused by earthquakes and explosions, accelerometers mounted on orbiting space vehicles measure vibrations in and of the vehicle due to internal and external sources, as well as vibrations in a sensor's relative acceleration with respect to the vehicle to which it is attached. The data collected over the years have helped to alter the perception of gravity on-board a space vehicle from the public's early concept of zero-gravity to the science community's evolution of thought from microgravity to milligravity to g-jitter or vibrational environment. Since the advent of the Shuttle Orbiter Program, especially since the start of Spacelab flights dedicated to scientific investigations, the interest in measuring the low-gravity environment in which experiments are run has increased. This interest led to the development and flight of numerous accelerometer systems dedicated to specific experiments. It also prompted the development of the NASA MSAD-sponsored Space Acceleration Measurement System (SAMS). The first SAMS units flew in the Spacelab on STS-40 in June 1991 in support of the first Spacelab Life Sciences mission (SLS-1). SAMS is currently manifested to fly on all future Spacelab missions.

Algal derivatives may protect crops from residual soil salinity: a case study on a tomato-wheat rotation

NASA Astrophysics Data System (ADS)

Di Stasio, Emilio; Raimondi, Giampaolo; Van Oosten, Michael; Maggio, Albino

2017-04-01

In coastal areas, summer crops are frequently irrigated with saline water. As a consequence, salts may accumulate in the root zone with detrimental effects on the following winter crops if the rainfall is insufficient to leach them. Two field experiments were performed in 2015-2016 on a field used for tomato (summer) wheat (winter) rotation cropping. The spring-summer experiment was carried in order to evaluate the effect of two algal derivatives (Ascophyllum nodosum), Rygex and Super Fifty, on a tomato crop exposed to increasing salinity and reduced nutrient availability. In the autumn-winter experiment we investigated the effect of residual salts from the previous summer irrigations on plant growth and yield of wheat treated with the same two algal extracts. The salt treatment for the irrigated summer crop was 80 mM NaCl plus a non-salinized control. The nutrient regimes were 100% and 50% of the tomato nutritional requirements. With both the seaweeds applications the salt stressed plants were demonstrated improved Relative Water Content and water potential. Nevertheless the total fresh biomass and the fruit fresh weight were enhanced only in the non salinized controls. Application of algal derivatives increased the total fresh weight over controls in the non salinized plants. The seaweed treatments enhanced the fruit fresh weight with an increase of 30% and 46% for Rygex and Super Fifty, respectively. Preliminary analysis of the ion profile in roots, shoots and leaves, indicates that the seaweed extracts may enhance the assimilation of ions in fruits affecting their nutritional value. The residual salinity of the summer experiment reduced the wheat biomass production. However, the seaweed extracts treatments improved growth under salinity. In the salt stressed plants the Super Fifty application increased shoots and ears by 34% and 23% respectively, compared to the non treated plants. Plant height was increased by application of seaweeds extracts for both the

All auto shredding: evaluation of automotive shredder residue generated by shredding only vehicles.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Duranceau, C. M.; Spangenberger, J. S.; Vehicle Recycling Partnership, LLC)

A well developed infrastructure exists for the reuse and recycling of automotive parts and materials. At the end of a vehicle's useful life many parts are removed and sold for reuse and fluids are recovered for recycling or proper disposal. What remains is shredded, along with other metal bearing scrap such as home appliances, demolition debris and process equipment, and the metals are separated out and recycled. The remainder of the vehicle materials is call shredder residue which ends up in the landfill. As energy and natural resources becomes more treasured, increased effort has been afforded to find ways tomore » reduce energy consumption and minimize the use of our limited resources. Many of the materials found in shredder residue could be recovered and help offset the use of energy and material consumption. For example, the energy content of the plastics and rubbers currently landfilled with the shredder residue is equivalent to 16 million barrels of oil per year. However, in the United States, the recovered materials, primarily polymers, cannot be recycled due to current regulatory barriers which preclude the re-introduction into commerce of certain materials because of residual contamination with substances of concern (SOCs) such as polychlorinated biphenyls (PCBs). The source of the PCBs is not well understood. Old transformers, capacitors, white goods and ballasts from lighting fixtures are likely contributing factors. The project was designed to evaluate whether vehicles of varying age and manufacturing origin contribute to the PCB content in shredder residue. Additionally, the project was designed to determine if there are any trends in material composition of the shredder residue from varied age and manufacturing groups. This information would aid in future material recovery facility strategy and design. The test utilized a newly installed shredder plant to shred four categories of automobiles. The categories were defined by vehicle age and the

Residual Efficacy of Field-Applied Permethrin, d-Phenothrin, and Resmethrin on Plant Foliage Against Adult Mosquitoes

DTIC Science & Technology

2008-01-01

Kerst WC. 1997. Residue levels of pyrethrins and piperonyl butoxide in soil and runoff water. J Environ Sci Health B 32:621–644. Antonious GF, Snyder JC...Patel GA. 2001. Pyrethrins and piperonyl butoxide residues on potato leaves and in soil under field conditions. J Environ Sci Health B 36:261–271... metabolism of the pyrethroids cis- and trans- cypermethrin in rainbow trout, Salmo gairdneri. Xenobiotica 17:1175–1193. Foster WA. 1995. Mosquito sugar feeding

Identification of Key Residues for pH Dependent Activation of Violaxanthin De-Epoxidase from Arabidopsis thaliana

PubMed Central

Fufezan, Christian; Simionato, Diana; Morosinotto, Tomas

2012-01-01

Plants are often exposed to saturating light conditions, which can lead to oxidative stress. The carotenoid zeaxanthin, synthesized from violaxanthin by Violaxanthin De-Epoxidase (VDE) plays a major role in the protection from excess illumination. VDE activation is triggered by a pH reduction in the thylakoids lumen occurring under saturating light. In this work the mechanism of the VDE activation was investigated on a molecular level using multi conformer continuum electrostatic calculations, site directed mutagenesis and molecular dynamics. The pKa values of residues of the inactive VDE were determined to identify target residues that could be implicated in the activation. Five such target residues were investigated closer by site directed mutagenesis, whereas variants in four residues (D98, D117, H168 and D206) caused a reduction in enzymatic activity indicating a role in the activation of VDE while D86 mutants did not show any alteration. The analysis of the VDE sequence showed that the four putative activation residues are all conserved in plants but not in diatoms, explaining why VDE in these algae is already activated at higher pH. Molecular dynamics showed that the VDE structure was coherent at pH 7 with a low amount of water penetrating the hydrophobic barrel. Simulations carried out with the candidate residues locked into their protonated state showed instead an increased amount of water penetrating the barrel and the rupture of the H121–Y214 hydrogen bond at the end of the barrel, which is essential for VDE activation. These results suggest that VDE activation relies on a robust and redundant network, in which the four residues identified in this study play a major role. PMID:22558195

Identification of key residues for pH dependent activation of violaxanthin de-epoxidase from Arabidopsis thaliana.

PubMed

Fufezan, Christian; Simionato, Diana; Morosinotto, Tomas

2012-01-01

Plants are often exposed to saturating light conditions, which can lead to oxidative stress. The carotenoid zeaxanthin, synthesized from violaxanthin by Violaxanthin De-Epoxidase (VDE) plays a major role in the protection from excess illumination. VDE activation is triggered by a pH reduction in the thylakoids lumen occurring under saturating light. In this work the mechanism of the VDE activation was investigated on a molecular level using multi conformer continuum electrostatic calculations, site directed mutagenesis and molecular dynamics. The pK(a) values of residues of the inactive VDE were determined to identify target residues that could be implicated in the activation. Five such target residues were investigated closer by site directed mutagenesis, whereas variants in four residues (D98, D117, H168 and D206) caused a reduction in enzymatic activity indicating a role in the activation of VDE while D86 mutants did not show any alteration. The analysis of the VDE sequence showed that the four putative activation residues are all conserved in plants but not in diatoms, explaining why VDE in these algae is already activated at higher pH. Molecular dynamics showed that the VDE structure was coherent at pH 7 with a low amount of water penetrating the hydrophobic barrel. Simulations carried out with the candidate residues locked into their protonated state showed instead an increased amount of water penetrating the barrel and the rupture of the H121-Y214 hydrogen bond at the end of the barrel, which is essential for VDE activation. These results suggest that VDE activation relies on a robust and redundant network, in which the four residues identified in this study play a major role.

Plant Metabolomics: An Indispensable System Biology Tool for Plant Science

PubMed Central

Hong, Jun; Yang, Litao; Zhang, Dabing; Shi, Jianxin

2016-01-01

As genomes of many plant species have been sequenced, demand for functional genomics has dramatically accelerated the improvement of other omics including metabolomics. Despite a large amount of metabolites still remaining to be identified, metabolomics has contributed significantly not only to the understanding of plant physiology and biology from the view of small chemical molecules that reflect the end point of biological activities, but also in past decades to the attempts to improve plant behavior under both normal and stressed conditions. Hereby, we summarize the current knowledge on the genetic and biochemical mechanisms underlying plant growth, development, and stress responses, focusing further on the contributions of metabolomics to practical applications in crop quality improvement and food safety assessment, as well as plant metabolic engineering. We also highlight the current challenges and future perspectives in this inspiring area, with the aim to stimulate further studies leading to better crop improvement of yield and quality. PMID:27258266

Plant Metabolomics: An Indispensable System Biology Tool for Plant Science.

PubMed

Hong, Jun; Yang, Litao; Zhang, Dabing; Shi, Jianxin

2016-06-01

As genomes of many plant species have been sequenced, demand for functional genomics has dramatically accelerated the improvement of other omics including metabolomics. Despite a large amount of metabolites still remaining to be identified, metabolomics has contributed significantly not only to the understanding of plant physiology and biology from the view of small chemical molecules that reflect the end point of biological activities, but also in past decades to the attempts to improve plant behavior under both normal and stressed conditions. Hereby, we summarize the current knowledge on the genetic and biochemical mechanisms underlying plant growth, development, and stress responses, focusing further on the contributions of metabolomics to practical applications in crop quality improvement and food safety assessment, as well as plant metabolic engineering. We also highlight the current challenges and future perspectives in this inspiring area, with the aim to stimulate further studies leading to better crop improvement of yield and quality.

Tannase production by Penicillium purpurogenum PAF6 in solid state fermentation of tannin-rich plant residues following OVAT and RSM.

PubMed

Jana, Arijit; Maity, Chiranjit; Halder, Suman Kumar; Mondal, Keshab Chandra; Pati, Bikash Ranjan; Mohapatra, Pradeep Kumar Das

2012-07-01

Tannase production by newly isolated Penicillium purpurogenum PAF6 was investigated by 'one variable at a time' (OVAT) approach followed by response surface methodology (RSM). Tannin-rich plant residues were used as supporting solid substrate and sole carbon source and, among them, tamarind seed was found to be the most favorable substrate than haritaki, pomegranate, tea leaf waste and arjun fruit. Physicochemical parameters were initially optimized using OVAT methodology and some important factors like incubation time, incubation temperature, substrate:moisture ratio as well as carbon, nitrogen and phosphate concentrations were verified with Box-Behken design of response surface methodology. Phosphate source, nitrogen source and temperature were found as the most favorable variables in the maximization of production. Tannase production was enhanced from 1.536 U/g to 5.784 U/g using tamarind seed OVAT optimization and further enhancement up to 6.15 U/g following RSM. An overall 3.76- and 4.0-fold increases in tannase production were achieved in OVAT and RSM, respectively.

Crop residues quantification to obtain self-consumption compost in an organic garden

NASA Astrophysics Data System (ADS)

Lopez de Fuentes, Pilar; Lopez Merino, María; Remedios Alvir, María; Briz de Felipe, Teresa

2013-04-01

This research focuses on quantifying the crop residue left after the campaign fall/winter (2011) for the organic garden crops of Agricultural ETSI, located in practice fields, to get compost for self-generated residues arising from within their own fields. This compost is produced by mixing this material with an organic residues source animal. In this way the plant organic residues provided the nitrogen required for an appropriate C/N and the animal organic residues can provide the carbon amount required to achieve an optimal scenario. The garden has a surface area of 180 m2 which was cultured with different seasonal vegetables, different families and attending practices and species associations' rotations, proper of farming techniques. The organic material of animal origin referred to, is rest from sheep renew bed, sustained management support the precepts of organic farming and cottage belongs to practice fields too. At the end of crop cycle, we proceeded to the harvest and sorting of usable crop residues, which was considered as net crop residues. In each case, these residues were subjected to a cutting treatment by the action of a mincing machine and then weighed to estimate the amounts given by each crop. For the sheep bed residue 1m2 was collected after three months having renewed. It had been made by providing 84 kg of straw bales in July and introducing about 12 Kg each. The herd consisted of three females and one playe. Each one of them was feed 300g and 600 g of straw per day. Two alternating different pens were used to simulate a regime of semi-intensive housing. A balance on how much organic residue material was obtained at the end and how much was obtained in the compost process is discussed in terms of volume and nutrients content is discussed.

Molecular basis of the evolution of alternative tyrosine biosynthetic routes in plants

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schenck, Craig A.; Holland, Cynthia K.; Schneider, Matthew R.

L-Tyrosine (Tyr) is essential for protein synthesis and is a precursor of numerous specialized metabolites crucial for plant and human health. Tyr can be synthesized via two alternative routes by different key regulatory TyrA family enzymes, prephenate dehydrogenase (PDH, also known as TyrAp) or arogenate dehydrogenase (ADH, also known as TyrAa), representing a unique divergence of primary metabolic pathways. The molecular foundation underlying the evolution of these alternative Tyr pathways is currently unknown. Here we characterized recently diverged plant PDH and ADH enzymes, obtained the X-ray crystal structure of soybean PDH, and identified a single amino acid residue that definesmore » TyrA substrate specificity and regulation. Structures of mutated PDHs co-crystallized with Tyr indicate that substitutions of Asn222 confer ADH activity and Tyr sensitivity. Reciprocal mutagenesis of the corresponding residue in divergent plant ADHs further introduced PDH activity and relaxed Tyr sensitivity, highlighting the critical role of this residue in TyrA substrate specificity that underlies the evolution of alternative Tyr biosynthetic pathways in plants.« less

Preliminary determination of Newtonian gravitational constant with angular acceleration feedback method

PubMed Central

Xue, Chao; Quan, Li-Di; Yang, Shan-Qing; Wang, Bing-Peng; Wu, Jun-Fei; Shao, Cheng-Gang; Tu, Liang-Cheng; Milyukov, Vadim; Luo, Jun

2014-01-01

This paper describes the preliminary measurement of the Newtonian gravitational constant G with the angular acceleration feedback method at HUST. The apparatus has been built, and preliminary measurement performed, to test all aspects of the experimental design, particularly the feedback function, which was recently discussed in detail by Quan et al. The experimental results show that the residual twist angle of the torsion pendulum at the signal frequency introduces 0.4 ppm to the value of G. The relative uncertainty of the angular acceleration of the turntable is approximately 100 ppm, which is mainly limited by the stability of the apparatus. Therefore, the experiment has been modified with three features: (i) the height of the apparatus is reduced almost by half, (ii) the aluminium shelves were replaced with shelves made from ultra-low expansion material and (iii) a perfect compensation of the laboratory-fixed gravitational background will be carried out. With these improvements, the angular acceleration is expected to be determined with an uncertainty of better than 10 ppm, and a reliable value of G with 20 ppm or below will be obtained in the near future. PMID:25201996

Incineration of nuclear waste by accelerator

NASA Astrophysics Data System (ADS)

Martino, J.; Fioni, G.; Leray, S.

1998-10-01

An important international effort is devoted to find a suitable solution to incinerate radioactive nuclear waste issued from conventional power plants and from nuclear disarmament. Practically all innovative projects consist of a sub critical system driven by an external neutron source obtained by spallation induced by a high intensity proton accelerator irradiating a heavy target. New nuclear data measurements are necessary for the realization of these systems, in particular a good knowledge of the spallation process and of the neutron cross sections for transuranic elements are essential.

Evaluation of microbially enhanced composting of sophora flavescens residues.

PubMed

Wang, Hai B; Han, Li R; Feng, Jun T; Zhang, Xing

2016-02-01

The effects of inoculants on the composting of Sophora flavescens residues were evaluated based on several physical, chemical and biological parameters, as well as the infrared spectra. Compared to the control compost without inoculants, the treatment compost with inoculants (Bacillus subtilis strain G-13 and Chaetomium thermophilum strain GF-1) had a significantly longer thermophilic duration, higher cellulase activity and a higher degradation rate of cellulose, hemicellulose and lignin (P < 0.05). Thus, a higher maturity degree of compost with apparently lower C:N ratio (15.88 vs. 17.77) and NH 4 -N:NO 3 -N ratio (0.16 vs. 0.20) was obtained with the inoculation comparing with the control (P < 0.05). Besides, the inoculants could markedly accelerate the composting process and increase the maturity degree of compost as indicated by the germination index (GI) in which the treatment reached the highest GI of 133.2% at day 15 while the control achieved the highest GI of 125.7% at day 30 of the composting. Inoculation with B. subtilis and C. thermophilum is a useful method to enhance the S. flavescens residues composting according to this study.

Consumer exposure to pesticide residues in apples from the region of south-eastern Poland.

PubMed

Szpyrka, Ewa; Kurdziel, Anna; Słowik-Borowiec, Magdalena; Grzegorzak, Magdalena; Matyaszek, Aneta

2013-11-01

The production of apples in Poland is the largest among the countries of the European Union, and therefore, the consumption of these fruits is high in our country. The aim of this study was to determine the presence of pesticide residues in Polish apples and to assess if these residues pose a risk to the health of the consumer. Furthermore, compliance with legal regulations concerning the use of plant protection products in crop cultivation was ascertained. Pesticide residues were found in 192 samples (61.5% of tested samples). In six samples (1.9%), residues exceeded maximum residue limits. Violations concerned the insecticides: indoxacarb, diazinon and fenitrothion. The highest long-term consumer exposure was found in the case of consumption of apples with diazinon residue for both groups, adults and toddlers [4% acceptable daily intake (ADI), adults; 21% ADI, toddlers]. The highest values of short-term exposure were obtained in the case of consumption of apples with indoxacarb [5% acute reference dose (ARfD), adults; 27% ARfD, toddlers] and fenitrothion (4% ARfD, adults; 23% ARfD, toddlers). Although fungicides are the pesticides found most often in apples, the consumption of apples with insecticide residues constitutes the greatest hazard to human health.

Towards the identification of plant and animal binders on Australian stone knives.

PubMed

Blee, Alisa J; Walshe, Keryn; Pring, Allan; Quinton, Jamie S; Lenehan, Claire E

2010-07-15

There is limited information regarding the nature of plant and animal residues used as adhesives, fixatives and pigments found on Australian Aboriginal artefacts. This paper reports the use of FTIR in combination with the chemometric tools principal component analysis (PCA) and hierarchical clustering (HC) for the analysis and identification of Australian plant and animal fixatives on Australian stone artefacts. Ten different plant and animal residues were able to be discriminated from each other at a species level by combining FTIR spectroscopy with the chemometric data analysis methods, principal component analysis (PCA) and hierarchical clustering (HC). Application of this method to residues from three broken stone knives from the collections of the South Australian Museum indicated that two of the handles of knives were likely to have contained beeswax as the fixative whilst Spinifex resin was the probable binder on the third. Copyright 2010 Elsevier B.V. All rights reserved.

Observation of residues in tissues of chickens exposed to low dietary concentrations of chloramphenicol.

PubMed

Rejtharová, Martina; Rejthar, Libor; Čačková, Katarína; Bureš, Jiří; Vernerová, Eva; Hera, Alfred

2017-04-01

To investigate potential residues in tissues arising from naturally occurring low levels of chloramphenicol in plant material, feeding studies were conducted with chickens. A common chicken feed was prepared containing 0, 10, 50 and 200 μg kg -1 chloramphenicol and levels were confirmed by LC-MS/MS. Four separate groups of broiler chickens, eight animals in each group, were fed all their 35-day life with this contaminated feed. They were allowed ad libitum access to this feed and fresh water. After slaughtering the chickens, the residues in muscle and liver tissues were determined using GC/MS-NCI method. No residues were detected in tissues of animals from groups fed with feed containing 0, 10 or 50 µg kg -1 . Low chloramphenicol residual concentrations were observed in a few of the muscle samples obtained from the group of chickens fed with feed containing chloramphenicol in added concentration 200 µg kg -1 . No residues were detected in the remaining samples of this group. These results indicate that when residues of chloramphenicol are detected it is in all probability through illegal use.

Presence of pesticide residues on produce cultivated in Suriname.

PubMed

Abdoel Wahid, F; Wickliffe, J; Wilson, M; Van Sauers, A; Bond, N; Hawkins, W; Mans, D; Lichtveld, M

2017-06-01

Agricultural pesticides are widely used in Suriname, an upper middle-income Caribbean country located in South America. Suriname imported 1.8 million kg of agricultural pesticides in 2015. So far, however, national monitoring of pesticides in crops is absent. Reports from the Netherlands on imported Surinamese produce from 2010 to 2015 consistently showed that samples exceeded plant-specific pesticide maximum residue limits (MRLs) of the European Union (EU). Consumption of produce containing unsafe levels of pesticide residues can cause neurological disorders, and particularly, pregnant women and children may be vulnerable. This pilot study assessed the presence of pesticide residues in commonly consumed produce items cultivated in Suriname. Thirty-two insecticides (organophosphates, organochlorines, carbamates, and pyrethroids) and 12 fungicides were evaluated for their levels in nine types of produce. Pesticide residue levels exceeding MRLs in this study regarded cypermethrin (0.32 μg/g) in tomatoes (USA MRL 0.20 μg/g), lambda-cyhalothrin (1.08 μg/g) in Chinese cabbage (USA MRL 0.40 μg/g), endosulfan (0.07 μg/g) in tannia (EU MRL 0.05 μg/g), and lindane (0.02 and 0.03 μg/g, respectively) in tannia (EU MRL 0.01 μg/g). While only a few pesticide residues were detected in this small pilot study, these residues included two widely banned pesticides (endosulfan and lindane). There is a need to address environmental policy gaps. A more comprehensive sampling and analysis of produce from Suriname is warranted to better understand the scope of the problem. Preliminary assessments, using intake rate, hazard quotient, and level of concern showed that it is unlikely that daily consumption of tannia leads to adverse health effects.

Presence of pesticide residues on produce cultivated in Suriname

PubMed Central

Wickliffe, J.; Wilson, M.; Van Sauers, A.; Bond, N.; Hawkins, W.; Mans, D.; Lichtveld, M.

2017-01-01

Agricultural pesticides are widely used in Suriname, an upper middle-income Caribbean country located in South America. Suriname imported 1.8 million kg of agricultural pesticides in 2015. So far, however, national monitoring of pesticides in crops is absent. Reports from the Netherlands on imported Surinamese produce from 2010 to 2015 consistently showed that samples exceeded plant-specific pesticide maximum residue limits (MRLs) of the European Union (EU). Consumption of produce containing unsafe levels of pesticide residues can cause neurological disorders, and particularly, pregnant women and children may be vulnerable. This pilot study assessed the presence of pesticide residues in commonly consumed produce items cultivated in Suriname. Thirty-two insecticides (organophosphates, organochlorines, carbamates, and pyrethroids) and 12 fungicides were evaluated for their levels in nine types of produce. Pesticide residue levels exceeding MRLs in this study regarded cypermethrin (0.32 μg/g) in tomatoes (USA MRL 0.20 μg/g), lambda-cyhalothrin (1.08 μg/g) in Chinese cabbage (USA MRL 0.40 μg/g), endosulfan (0.07 μg/g) in tannia (EU MRL 0.05 μg/g), and lindane (0.02 and 0.03 μg/g, respectively) in tannia (EU MRL 0.01 μg/g). While only a few pesticide residues were detected in this small pilot study, these residues included two widely banned pesticides (endosulfan and lindane). There is a need to address environmental policy gaps. A more comprehensive sampling and analysis of produce from Suriname is warranted to better understand the scope of the problem. Preliminary assessments, using intake rate, hazard quotient, and level of concern showed that it is unlikely that daily consumption of tannia leads to adverse health effects. PMID:28567597

Evaluation of proton cross-sections for radiation sources in the proton accelerator

NASA Astrophysics Data System (ADS)

Cho, Young-Sik; Lee, Cheol-Woo; Lee, Young-Ouk

2007-08-01

Proton Engineering Frontier Project (PEFP) is currently building a proton accelerator in Korea which consists of a proton linear accelerator with 100 MeV of energy, 20 mA of current and various particle beam facilities. The final goal of this project consists of the production of 1 GeV proton beams, which will be used for various medical and industrial applications as well as for research in basic and applied sciences. Carbon and copper in the proton accelerator for PEPP, through activation, become radionuclides such as 7Be and 64Cu. Copper is a major element of the accelerator components and the carbon is planned to be used as a target material of the beam dump. A recent survey showed that the currently available cross-sections create a large difference from the experimental data in the production of some residual nuclides by the proton-induced reactions for carbon and copper. To more accurately estimate the production of radioactive nuclides in the accelerator, proton cross-sections for carbon and copper are evaluated. The TALYS code was used for the evaluation of the cross-sections for the proton-induced reactions. To obtain the cross-sections which best fits the experimental data, optical model parameters for the neutron, proton and other complex particles such as the deuteron and alpha were successively adjusted. The evaluated cross-sections in this study are compared with the measurements and other evaluations .

Optimal Model-Based Fault Estimation and Correction for Particle Accelerators and Industrial Plants Using Combined Support Vector Machines and First Principles Models

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sayyar-Rodsari, Bijan; Schweiger, Carl; /SLAC /Pavilion Technologies, Inc., Austin, TX

2010-08-25

model-based fault estimation and correction for particle accelerators and industrial plants feasible.« less

Covariant Uniform Acceleration

NASA Astrophysics Data System (ADS)

Friedman, Yaakov; Scarr, Tzvi

2013-04-01

We derive a 4D covariant Relativistic Dynamics Equation. This equation canonically extends the 3D relativistic dynamics equation , where F is the 3D force and p = m0γv is the 3D relativistic momentum. The standard 4D equation is only partially covariant. To achieve full Lorentz covariance, we replace the four-force F by a rank 2 antisymmetric tensor acting on the four-velocity. By taking this tensor to be constant, we obtain a covariant definition of uniformly accelerated motion. This solves a problem of Einstein and Planck. We compute explicit solutions for uniformly accelerated motion. The solutions are divided into four Lorentz-invariant types: null, linear, rotational, and general. For null acceleration, the worldline is cubic in the time. Linear acceleration covariantly extends 1D hyperbolic motion, while rotational acceleration covariantly extends pure rotational motion. We use Generalized Fermi-Walker transport to construct a uniformly accelerated family of inertial frames which are instantaneously comoving to a uniformly accelerated observer. We explain the connection between our approach and that of Mashhoon. We show that our solutions of uniformly accelerated motion have constant acceleration in the comoving frame. Assuming the Weak Hypothesis of Locality, we obtain local spacetime transformations from a uniformly accelerated frame K' to an inertial frame K. The spacetime transformations between two uniformly accelerated frames with the same acceleration are Lorentz. We compute the metric at an arbitrary point of a uniformly accelerated frame. We obtain velocity and acceleration transformations from a uniformly accelerated system K' to an inertial frame K. We introduce the 4D velocity, an adaptation of Horwitz and Piron s notion of "off-shell." We derive the general formula for the time dilation between accelerated clocks. We obtain a formula for the angular velocity of a uniformly accelerated object. Every rest point of K' is uniformly accelerated, and

Plant Biomass Leaching for Nutrient Recovery in Closed Loop Systems Project

NASA Technical Reports Server (NTRS)

Zeitlin, Nancy P.; Wheeler, Raymond (Compiler); Lunn, Griffin

2015-01-01

Plants will be important for food and O2 production during long term human habitation in space. Recycling of nutrients (e.g., from waste materials) could reduce the resupply costs of fertilizers for growing these plants. Work at NASA's Kennedy Space Center has shown that ion exchange resins can extract fertilizer (plant essential nutrients) from human waste water, after which the residual brine could be treated with electrodialysis to recover more water and produce high value chemicals (e.g., acids and bases). In habitats with significant plant production, inedible biomass becomes a major source of solid waste. To "close the loop" we also need to recover useful nutrients and fertilizer from inedible biomass. We are investigating different approaches to retrieve nutrients from inedible plant biomass, including physical leaching with water, processing the biomass in bioreactors, changing the pH of leaching processing, and/or conducting multiple leaches of biomass residues.

Spatial decision support system to evaluate crop residue energy potential by anaerobic digestion.

PubMed

Escalante, Humberto; Castro, Liliana; Gauthier-Maradei, Paola; Rodríguez De La Vega, Reynel

2016-11-01

Implementing anaerobic digestion (AD) in energy production from crop residues requires development of decision tools to assess its feasibility and sustainability. A spatial decision support system (SDSS) was constructed to assist decision makers to select appropriate feedstock according to biomethanation potential, identify the most suitable location for biogas facilities, determine optimum plant capacity and supply chain, and evaluate associated risks and costs. SDSS involves a spatially explicit analysis, fuzzy multi-criteria analysis, and statistical and optimization models. The tool was validated on seven crop residues located in Santander, Colombia. For example, fique bagasse generates about 0.21millionm(3)CH4year(-1) (0.329m(3)CH4kg(-1) volatile solids) with a minimum profitable plant of about 2000tonyear(-1) and an internal rate of return of 10.5%. SDSS can be applied to evaluate other biomass resources, availability periods, and co-digestion potential. Copyright © 2016. Published by Elsevier Ltd.

Initial Effects of Differently Treated Biogas Residues from Municipal and Industrial Wastes on Spring Barley Yield Formation

PubMed Central

Prays, Nadia; Kaupenjohann, Martin

2016-01-01

Soil application of biogas residues (BGRs) is important for closing nutrient cycles. This study examined the efficiency and impact on yields and yield formation of solid-liquid separated residues from biodegradable municipal and industrial wastes (bio-waste) in comparison to complete BGRs, nitrification inhibitor, agricultural BGRs, mineral fertilizer and unfertilized plots as control. The experiment was set up as a randomized block design on silt loam Cambisol. Biogas residues from four biogas plants were evaluated. Plants per m², ears per plant, grains per ear and thousand grain weight (TGW) were measured at harvest. Fertilization with BGRs resulted in similar biomass yields compared with mineral fertilizer. Mineral fertilizer (71 dt/ha) and plots fertilized with liquid fraction (59–62 dt/ha) indicated a trend to higher yields than solid fraction or complete BGR due to its high ammonia content. Liquid fractions and fraction with nitrification inhibitor induced fewer plants per m² than corresponding solid and complete variants due to a potential phytotoxicity of high NH4-N concentration during germination. However, barley on plots fertilized with liquid fraction compensated the disadvantages at the beginning during the vegetation period and induced higher grain yields than solid fraction. This was attributable to a higher number of ears per plant and grains per ear. In conclusion, BGRs from biodegradable municipal and industrial wastes can be used for soil fertilization and replace considerable amounts of mineral fertilizer. Our study showed that direct application of the liquid fraction of BGR is the most suitable strategy to achieve highest grain yields. Nevertheless potential phytotoxicity of the high NH4-N concentration in the liquid fraction should be considered. PMID:27116355

Analyzing radial acceleration with a smartphone acceleration sensor

NASA Astrophysics Data System (ADS)

Vogt, Patrik; Kuhn, Jochen

2013-03-01

This paper continues the sequence of experiments using the acceleration sensor of smartphones (for description of the function and the use of the acceleration sensor, see Ref. 1) within this column, in this case for analyzing the radial acceleration.

Assessing the levels of food shortage using the traffic light metaphor by analyzing the gathering and consumption of wild food plants, crop parts and crop residues in Konso, Ethiopia

PubMed Central

2012-01-01

Background Humanitarian relief agencies use scales to assess levels of critical food shortage to efficiently target and allocate food to the neediest. These scales are often labor-intensive. A lesser used approach is assessing gathering and consumption of wild food plants. This gathering per se is not a reliable signal of emerging food stress. However, the gathering and consumption of some specific plant species could be considered markers of food shortage, as it indicates that people are compelled to eat very poor or even health-threatening food. Methods We used the traffic light metaphor to indicate normal (green), alarmingly low (amber) and fully depleted (red) food supplies and identified these conditions for Konso (Ethiopia) on the basis of wild food plants (WFPs), crop parts (crop parts not used for human consumption under normal conditions; CPs) and crop residues (CRs) being gathered and consumed. Plant specimens were collected for expert identification and deposition in the National Herbarium. Two hundred twenty individual households free-listed WFPs, CPs, and CRs gathered and consumed during times of food stress. Through focus group discussions, the species list from the free-listing that was further enriched through key informants interviews and own field observations was categorized into species used for green, amber and red conditions. Results The study identified 113 WFPs (120 products/food items) whose gathering and consumption reflect the three traffic light metaphors: red, amber and green. We identified 25 food items for the red, 30 food items for the amber and 65 food items for the green metaphor. We also obtained reliable information on 21 different products/food items (from 17 crops) normally not consumed as food, reflecting the red or amber metaphor and 10 crop residues (from various crops), plus one recycled stuff which are used as emergency foods in the study area clearly indicating the severity of food stress (red metaphor) households are

Residual translation compensations in radar target narrowband imaging based on trajectory information

NASA Astrophysics Data System (ADS)

Yue, Wenjue; Peng, Bo; Wei, Xizhang; Li, Xiang; Liao, Dongping

2018-05-01

High velocity translation will result in defocusing scattering centers in radar imaging. In this paper, we propose a Residual Translation Compensations (RTC) method based on target trajectory information to eliminate the translation effects in radar imaging. Translation could not be simply regarded as a uniformly accelerated motion in reality. So the prior knowledge of the target trajectory is introduced to enhance compensation precision. First we use the two-body orbit model to figure out the radial distance. Then, stepwise compensations are applied to eliminate residual propagation delay based on conjugate multiplication method. Finally, tomography is used to confirm the validity of the method. Compare with translation parameters estimation method based on the spectral peak of the conjugate multiplied signal, RTC method in this paper enjoys a better tomography result. When the Signal Noise Ratio (SNR) of the radar echo signal is 4dB, the scattering centers can also be extracted clearly.

40 CFR 180.519 - Bromide ion and residual bromine; tolerances for residues.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Bromide ion and residual bromine... Tolerances § 180.519 Bromide ion and residual bromine; tolerances for residues. (a) General. The food additives, bromide ion and residual bromine, may be present in water, potable in accordance with the...

40 CFR 180.519 - Bromide ion and residual bromine; tolerances for residues.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 24 2011-07-01 2011-07-01 false Bromide ion and residual bromine... Tolerances § 180.519 Bromide ion and residual bromine; tolerances for residues. (a) General. The food additives, bromide ion and residual bromine, may be present in water, potable in accordance with the...

Detection of organic residues on poultry processing equipment surfaces by LED-induced fluorescence imaging

USDA-ARS?s Scientific Manuscript database

Organic residues on equipment surfaces in poultry processing plants can generate cross- contamination and increase the risk of unsafe food for consumers. This research was aimed to investigate the potential of LED-induced fluorescence imaging technique for rapid inspection of stainless steel proces...

Community Petascale Project for Accelerator Science and Simulation: Advancing Computational Science for Future Accelerators and Accelerator Technologies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Spentzouris, P.; /Fermilab; Cary, J.

The design and performance optimization of particle accelerators are essential for the success of the DOE scientific program in the next decade. Particle accelerators are very complex systems whose accurate description involves a large number of degrees of freedom and requires the inclusion of many physics processes. Building on the success of the SciDAC-1 Accelerator Science and Technology project, the SciDAC-2 Community Petascale Project for Accelerator Science and Simulation (ComPASS) is developing a comprehensive set of interoperable components for beam dynamics, electromagnetics, electron cooling, and laser/plasma acceleration modelling. ComPASS is providing accelerator scientists the tools required to enable the necessarymore » accelerator simulation paradigm shift from high-fidelity single physics process modeling (covered under SciDAC1) to high-fidelity multiphysics modeling. Our computational frameworks have been used to model the behavior of a large number of accelerators and accelerator R&D experiments, assisting both their design and performance optimization. As parallel computational applications, the ComPASS codes have been shown to make effective use of thousands of processors. ComPASS is in the first year of executing its plan to develop the next-generation HPC accelerator modeling tools. ComPASS aims to develop an integrated simulation environment that will utilize existing and new accelerator physics modules with petascale capabilities, by employing modern computing and solver technologies. The ComPASS vision is to deliver to accelerator scientists a virtual accelerator and virtual prototyping modeling environment, with the necessary multiphysics, multiscale capabilities. The plan for this development includes delivering accelerator modeling applications appropriate for each stage of the ComPASS software evolution. Such applications are already being used to address challenging problems in accelerator design and optimization. The Com

Residual herbicide study on selected Hanford Site roadsides

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, J.L.; Kemp, C.J.; Sackschewsky, M.R.

Westinghouse Hanford Company routinely treats roadsides with herbicides to control undesirable plant growth. An experiment was conducted to test perennial grass germination in soils adjacent to roadways of the Hanford Site. The primary variable was the distance from the roadside. A simple germination test was executed in a controlled-environment chamber to determine the residual effects of these applications. As expected, the greatest herbicide activity was found directly adjacent to the roadway, approximately 0 to 20 ft (0 to 6.3 m) from the roadway.

Super-Resolution of Plant Disease Images for the Acceleration of Image-based Phenotyping and Vigor Diagnosis in Agriculture

PubMed Central

Togami, Takashi; Yamaguchi, Norio

2017-01-01

Unmanned aerial vehicles (UAVs or drones) are a very promising branch of technology, and they have been utilized in agriculture—in cooperation with image processing technologies—for phenotyping and vigor diagnosis. One of the problems in the utilization of UAVs for agricultural purposes is the limitation in flight time. It is necessary to fly at a high altitude to capture the maximum number of plants in the limited time available, but this reduces the spatial resolution of the captured images. In this study, we applied a super-resolution method to the low-resolution images of tomato diseases to recover detailed appearances, such as lesions on plant organs. We also conducted disease classification using high-resolution, low-resolution, and super-resolution images to evaluate the effectiveness of super-resolution methods in disease classification. Our results indicated that the super-resolution method outperformed conventional image scaling methods in spatial resolution enhancement of tomato disease images. The results of disease classification showed that the accuracy attained was also better by a large margin with super-resolution images than with low-resolution images. These results indicated that our approach not only recovered the information lost in low-resolution images, but also exerted a beneficial influence on further image analysis. The proposed approach will accelerate image-based phenotyping and vigor diagnosis in the field, because it not only saves time to capture images of a crop in a cultivation field but also secures the accuracy of these images for further analysis. PMID:29113104

Examining the Potential of Forest Residue-Based Amendments for Post-Wildfire Rehabilitation in Colorado, USA

PubMed Central

Minatre, Kerri L.; Pierson, Derek N.; Fegel, Timothy S.; Cotrufo, M. Francesca; Kelly, Eugene F.

2017-01-01

Wildfire is a natural disturbance, though elemental losses and changes that occur during combustion and post-fire erosion can have long-term impacts on soil properties, ecosystem productivity, and watershed condition. Here we evaluate the potential of forest residue-based materials to rehabilitate burned soils. We compare soil nutrient and water availability, and plant recovery after application of 37 t ha−1 of wood mulch, 20 t ha−1 of biochar, and the combination of the two amendments with untreated, burned soils. We also conducted a greenhouse trial to examine how biochar influenced soil nutrient and water content under two wetting regimes. The effects of wood mulch on plant-available soil N and water content were significant and seasonally consistent during the three-year field study. Biochar applied alone had few effects under field conditions, but significantly increased soil pH, Ca, P, and water in the greenhouse. The mulched biochar treatment had the greatest effects on soil N and water availability and increased cover of the most abundant native plant. We found that rehabilitation treatments consisting of forest residue-based products have potential to enhance soil N and water dynamics and plant recovery following severe wildfire and may be justified where erosion risk or water supply protection are crucial. PMID:28321358

Examining the Potential of Forest Residue-Based Amendments for Post-Wildfire Rehabilitation in Colorado, USA.

PubMed

Rhoades, Charles C; Minatre, Kerri L; Pierson, Derek N; Fegel, Timothy S; Cotrufo, M Francesca; Kelly, Eugene F

2017-01-01

Wildfire is a natural disturbance, though elemental losses and changes that occur during combustion and post-fire erosion can have long-term impacts on soil properties, ecosystem productivity, and watershed condition. Here we evaluate the potential of forest residue-based materials to rehabilitate burned soils. We compare soil nutrient and water availability, and plant recovery after application of 37 t ha -1 of wood mulch, 20 t ha -1 of biochar, and the combination of the two amendments with untreated, burned soils. We also conducted a greenhouse trial to examine how biochar influenced soil nutrient and water content under two wetting regimes. The effects of wood mulch on plant-available soil N and water content were significant and seasonally consistent during the three-year field study. Biochar applied alone had few effects under field conditions, but significantly increased soil pH, Ca, P, and water in the greenhouse. The mulched biochar treatment had the greatest effects on soil N and water availability and increased cover of the most abundant native plant. We found that rehabilitation treatments consisting of forest residue-based products have potential to enhance soil N and water dynamics and plant recovery following severe wildfire and may be justified where erosion risk or water supply protection are crucial.

Accelerators, Beams And Physical Review Special Topics - Accelerators And Beams

DOE Office of Scientific and Technical Information (OSTI.GOV)

Siemann, R.H.; /SLAC

Accelerator science and technology have evolved as accelerators became larger and important to a broad range of science. Physical Review Special Topics - Accelerators and Beams was established to serve the accelerator community as a timely, widely circulated, international journal covering the full breadth of accelerators and beams. The history of the journal and the innovations associated with it are reviewed.

Commnity Petascale Project for Accelerator Science And Simulation: Advancing Computational Science for Future Accelerators And Accelerator Technologies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Spentzouris, Panagiotis; /Fermilab; Cary, John

The design and performance optimization of particle accelerators are essential for the success of the DOE scientific program in the next decade. Particle accelerators are very complex systems whose accurate description involves a large number of degrees of freedom and requires the inclusion of many physics processes. Building on the success of the SciDAC-1 Accelerator Science and Technology project, the SciDAC-2 Community Petascale Project for Accelerator Science and Simulation (ComPASS) is developing a comprehensive set of interoperable components for beam dynamics, electromagnetics, electron cooling, and laser/plasma acceleration modelling. ComPASS is providing accelerator scientists the tools required to enable the necessarymore » accelerator simulation paradigm shift from high-fidelity single physics process modeling (covered under SciDAC1) to high-fidelity multiphysics modeling. Our computational frameworks have been used to model the behavior of a large number of accelerators and accelerator R&D experiments, assisting both their design and performance optimization. As parallel computational applications, the ComPASS codes have been shown to make effective use of thousands of processors.« less

Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.

PubMed

O'Rourke, Christina; Gregson, Timothy; Murray, Lorna; Sadler, Ian H; Fry, Stephen C

2015-08-01

During evolution, plants have acquired and/or lost diverse sugar residues as cell-wall constituents. Of particular interest are primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors. Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis). 'Pectins' and 'hemicelluloses', operationally defined as extractable in oxalate (100 °C) and 6 m NaOH (37 °C), respectively, were acid- or Driselase-hydrolysed, and the monosaccharides analysed chromatographically. One unusual monosaccharide, 'U', was characterized by (1)H/(13)C-nuclear magnetic resonance spectroscopy and also enzymically. 'U' was identified as 3-O-methyl-D-galactose (3-MeGal). All pectins, except in Klebsormidium, contained acid- and Driselase-releasable galacturonate, suggesting homogalacturonan. All pectins, without exception, released rhamnose and galactose on acid hydrolysis; however, only in 'higher' charophytes (Charales, Coleochaetales) and Anthoceros were these sugars also efficiently released by Driselase, suggesting rhamnogalacturonan-I. Pectins of 'higher' charophytes, especially Chara, contained little arabinose, instead possessing 3-MeGal. Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses. Homogalacturonan is an ancient streptophyte feature, albeit secondarily lost in Klebsormidium. When conquering the land, the first embryophytes already possessed rhamnogalacturonan-I. In contrast, charophyte and land-plant hemicelluloses differ substantially, indicating major changes during terrestrialization. The presence of 3

Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-d-galactose residues

PubMed Central

O’Rourke, Christina; Gregson, Timothy; Murray, Lorna; Sadler, Ian H.; Fry, Stephen C.

2015-01-01

Background and Aims During evolution, plants have acquired and/or lost diverse sugar residues as cell-wall constituents. Of particular interest are primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors. Methods Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis). ‘Pectins’ and ‘hemicelluloses’, operationally defined as extractable in oxalate (100 °C) and 6 m NaOH (37 °C), respectively, were acid- or Driselase-hydrolysed, and the monosaccharides analysed chromatographically. One unusual monosaccharide, ‘U’, was characterized by 1H/13C-nuclear magnetic resonance spectroscopy and also enzymically. Key Results ‘U’ was identified as 3-O-methyl-d-galactose (3-MeGal). All pectins, except in Klebsormidium, contained acid- and Driselase-releasable galacturonate, suggesting homogalacturonan. All pectins, without exception, released rhamnose and galactose on acid hydrolysis; however, only in ‘higher’ charophytes (Charales, Coleochaetales) and Anthoceros were these sugars also efficiently released by Driselase, suggesting rhamnogalacturonan-I. Pectins of ‘higher’ charophytes, especially Chara, contained little arabinose, instead possessing 3-MeGal. Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses. Conclusions Homogalacturonan is an ancient streptophyte feature, albeit secondarily lost in Klebsormidium. When conquering the land, the first embryophytes already possessed rhamnogalacturonan-I. In contrast, charophyte and land-plant hemicelluloses differ

Studies of industrial emissions by accelerator-based techniques: A review of applications at CEDAD

NASA Astrophysics Data System (ADS)

Calcagnile, L.; Quarta, G.

2012-04-01

Different research activities are in progress at the Centre for Dating and Diagnostics (CEDAD), University of Salento, in the field of environmental monitoring by exploiting the potentialities given by the different experimental beam lines implemented on the 3 MV Tande-tron accelerator and dedicated to AMS (Accelerator Mass Spectrome-try) radiocarbon dating and IB A (Ion Beam Analysis). An overview of these activities is presented by showing how accelerator-based analytical techniques can be a powerful tool for monitoring the anthropogenic carbon dioxide emissions from industrial sources and for the assessment of the biogenic content in SRF (Solid Recovered Fuel) burned in WTE (Waste to Energy) plants.

Basic Residues of β-Sheet A Contribute to Heparin Binding and Activation of Vaspin (Serpin A12).

PubMed

Ulbricht, David; Oertwig, Kathrin; Arnsburg, Kristin; Saalbach, Anja; Pippel, Jan; Sträter, Norbert; Heiker, John T

2017-01-20

Many members of the serine protease inhibitor (serpin) family are activated by glycosaminoglycans (GAGs). Visceral adipose tissue-derived serpin (vaspin), serpin A12 of the serpin family, and its target protease kallikrein 7 (KLK7) are heparin-binding proteins, and inhibition of KLK7 by vaspin is accelerated by heparin. However, the nature of GAG binding to vaspin is not known. Here, we measured vaspin binding of various glycosaminoglycans and low molecular weight heparins by microscale thermophoresis and analyzed acceleration of protease inhibition by these molecules. In addition, basic residues contributing to heparin binding and heparin activation were identified by a selective labeling approach. Together, these data show that vaspin binds heparin with high affinity (K D = 21 ± 2 nm) and that binding takes place at a basic patch on top of β-sheet A and is different from other heparin-binding serpins. Mutation of basic residues decreased heparin binding and activation of vaspin. Similarly, reactive center loop insertion into sheet A decreased heparin binding because it disturbs the basic cluster. Finally, using vaspin-overexpressing keratinocyte cells, we show that a significant part of secreted vaspin is bound in the extracellular matrix on the cell surface. Together, basic residues of central β-sheet A contribute to heparin binding and activation of vaspin. Thus, binding to GAGs in the extracellular matrix can direct and regulate vaspin interaction with target proteases or other proteins and may play an important role in the various beneficial functions of vaspin in different tissues. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Through-Thickness Residual Stress Profiles in Austenitic Stainless Steel Welds: A Combined Experimental and Prediction Study

NASA Astrophysics Data System (ADS)

Mathew, J.; Moat, R. J.; Paddea, S.; Francis, J. A.; Fitzpatrick, M. E.; Bouchard, P. J.

2017-12-01

Economic and safe management of nuclear plant components relies on accurate prediction of welding-induced residual stresses. In this study, the distribution of residual stress through the thickness of austenitic stainless steel welds has been measured using neutron diffraction and the contour method. The measured data are used to validate residual stress profiles predicted by an artificial neural network approach (ANN) as a function of welding heat input and geometry. Maximum tensile stresses with magnitude close to the yield strength of the material were observed near the weld cap in both axial and hoop direction of the welds. Significant scatter of more than 200 MPa was found within the residual stress measurements at the weld center line and are associated with the geometry and welding conditions of individual weld passes. The ANN prediction is developed in an attempt to effectively quantify this phenomenon of `innate scatter' and to learn the non-linear patterns in the weld residual stress profiles. Furthermore, the efficacy of the ANN method for defining through-thickness residual stress profiles in welds for application in structural integrity assessments is evaluated.

Acceleration of the herbicide isoproturon degradation in wheat by glycosyltransferases and salicylic acid.

PubMed

Lu, Yi Chen; Zhang, Shuang; Yang, Hong

2015-01-01

Isoproturon (IPU) is a herbicide widely used to prevent weeds in cereal production. Due to its extensive use in agriculture, residues of IPU are often detected in soils and crops. Overload of IPU to crops is associated with human health risks. Hence, there is an urgent need to develop an approach to mitigate its accumulation in crops. In this study, the IPU residues and its degradation products in wheat were characterized using ultra performance liquid chromatography-time of fight tandem-mass spectrometer/mass spectrometer (UPLC-TOF-MS/MS). Most detected IPU-derivatives were sugar-conjugated. Degradation and glycosylation of IPU-derivatives could be enhanced by applying salicylic acid (SA). While more sugar-conjugated IPU-derivatives were identified in wheat with SA application, lower levels of IPU were detected, indicating that SA is able to accelerate intracellular IPU catabolism. All structures of IPU-derivatives and sugar-conjugated products were characterized. Comparative data were provided with specific activities and gene expression of certain glucosyltransferases. A pathway with IPU degradation and glucosylation was discussed. Our work indicates that SA-accelerated degradation is practically useful for wheat crops growing in IPU-contaminated soils because such crops with SA application can potentially lower or minimize IPU accumulation in levels below the threshold for adverse effects. Copyright © 2014 Elsevier B.V. All rights reserved.

Effects of introducing a single charged residue into the phenylalanine clamp of multimeric anthrax protective antigen.

PubMed

Janowiak, Blythe E; Fischer, Audrey; Collier, R John

2010-03-12

Multimeric pores formed in the endosomal membrane by the Protective Antigen moiety of anthrax toxin translocate the enzymatic moieties of the toxin to the cytosolic compartment of mammalian cells. There is evidence that the side chains of the Phe(427) residues come into close proximity with one another in the lumen of the pore and form a structure, termed the Phe clamp, that catalyzes the translocation process. In this report we describe the effects of replacing Phe(427) in a single subunit of the predominantly heptameric pore with a basic or an acidic amino acid. Incorporating any charged residue at this position inhibited cytotoxicity >or=1,000-fold in our standard assay and caused strong inhibition of translocation in a planar phospholipid bilayer system. His and Glu were the most strongly inhibitory residues, ablating both cytotoxicity and translocation. Basic residues at position 427 prevented the Phe clamp from interacting with a translocation substrate to form a seal against the passage of ions and accelerated dissociation of the substrate from the pore. Acidic residues, in contrast, allowed the seal to form and the substrate to remain firmly bound, but blocked its passage, perhaps via electrostatic interactions with the positively charged N-terminal segment. Our findings are discussed in relation to the role of the Phe clamp in a Brownian ratchet model of translocation.

Fermilab | Tevatron | Accelerator

Science.gov Websites

Leading accelerator technology Accelerator complex Illinois Accelerator Research Center Fermilab temperature. They were used to transfer particles from one part of the Fermilab accelerator complex to another center ring of Fermilab's accelerator complex. Before the Tevatron shut down, it had three primary

Focused Ion Beam Recovery of Hypervelocity Impact Residue in Experimental Craters on Metallic Foils

NASA Technical Reports Server (NTRS)

Graham, G. A.; Teslich, N.; Dai, Z. R.; Bradley, J. P.; Kearsley, A. T.; Horz, F.

2006-01-01

The Stardust sample return capsule will return to Earth in January 2006 with primitive debris collected from Comet 81P/Wild-2 during the fly-by encounter in 2004. In addition to the cometary particles embedded in low-density silica aerogel, there will be microcraters preserved in the Al foils (1100 series; 100 micrometers thick) that are wrapped around the sample tray assembly. Soda lime spheres (approximately 49 m in diameter) have been accelerated with a light-gas-gun into flight-grade Al foils at 6.35 km s(sup -1) to simulate the potential capture of cometary debris. The preserved crater penetrations have been analyzed using scanning electron microscopy (SEM) and x-ray energy dispersive spectroscopy (EDX) to locate and characterize remnants of the projectile material remaining within the craters. In addition, ion beam induced secondary electron imaging has proven particularly useful in identifying areas within the craters that contain residue material. Finally, high-precision focused ion beam (FIB) milling has been used to isolate and then extract an individual melt residue droplet from the interior wall of an impact penetration. This enabled further detailed elemental characterization, free from the background contamination of the Al foil substrate. The ability to recover pure melt residues using FIB will significantly extend the interpretations of the residue chemistry preserved in the Al foils returned by Stardust.

Residue analysis and persistence evaluation of fipronil and its metabolites in cotton using high-performance liquid chromatography-tandem mass spectrometry.

PubMed

Wu, Xiaohu; Yu, Yang; Xu, Jun; Dong, Fengshou; Liu, Xingang; Du, Pengqiang; Wei, Dongmei; Zheng, Yongquan

2017-01-01

A simple residue analytical method based on the QuEChERS approach and high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) detection was developed for the analysis of fipronil and its three metabolites in cottonseed, cotton plant and soil. The average recoveries of four test compounds from all three matrices were 78.6-108.9% at the level of 0.005 to 0.5 mg/kg, with an RSD in the range of 0.6 to 13.7%. The limit of quantification (LOQ) of the four test compounds ranged from 0.005 to 0.01 mg/kg. The results of the residual dynamics experiments showed that fipronil dissipated rapidly in cotton plants and soil and that oxidation and photolysis were the main degradation pathways. Moreover, the bi-exponential models demonstrated a good fit of the measured data for fipronil in cotton plants and soil, with R2 in the range of 0.8989 to 0.9989. Furthermore, a total of 40 samples of cottonseed from Shandong Province were analyzed, and all of the samples were free from the four test compound residues.

Residue analysis and persistence evaluation of fipronil and its metabolites in cotton using high-performance liquid chromatography-tandem mass spectrometry