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Sample records for accommodated fraction waf

  1. Acute and chronic toxicity study of the water accommodated fraction (WAF), chemically enhanced WAF (CEWAF) of crude oil and dispersant in the rock pool copepod Tigriopus japonicus.

    PubMed

    Lee, Kyun-Woo; Shim, Won Joon; Yim, Un Hyuk; Kang, Jung-Hoon

    2013-08-01

    We determined the toxicity of the water accommodated hydrocarbon fraction (WAF), two chemically enhanced WAFs (CEWAFs; CEWAF-C, Crude oil+Corexit 9500 and CEWAF-H, Crude oil+Hiclean) of crude oil and two dispersants (Corexit 9500 and Hiclean) to the rock pool copepod Tigriopus japonicus. In the acute toxicity test, Corexit 9500 was the most toxic of all the chemicals studied. The nauplius stage of T. japonicus was more susceptible to the toxic chemicals studied than the adult female. The toxicity data using the nauplius stage was then considered as baseline to determine the spiking concentration of chemicals for chronic toxicity tests on the copepod. As the endpoints in the chronic toxicity test, survival, sex ratio, developmental time and fecundity of the copepod were used. All chemicals used in this study resulted in increased toxicity in the F1 generation. The lowest-observed-adverse-effect (LOAE) concentrations of WAF, CEWAF-H, CEWAF-C, Hiclean and Corexit 9500 were observed to be 50%, 10%, 0.1%, 1% and 1%, respectively. The results in present study imply that copepods in marine may be negatively influenced by spilled oil and dispersant.

  2. Chemical comparison and acute toxicity of water accommodated fraction (WAF) of source and field collected Macondo oils from the Deepwater Horizon spill.

    PubMed

    Faksness, Liv-Guri; Altin, Dag; Nordtug, Trond; Daling, Per S; Hansen, Bjørn Henrik

    2015-02-15

    Two Source oils and five field collected oil residues from the Deepwater Horizon incident were chemically characterized. Water accommodated fractions (WAFs) of the Source oils and two of the field-weathered oils were prepared to evaluate the impact of natural weathering on the chemical composition and the acute toxicity of the WAFs. Toxicity test species representing different tropic levels were used (the primary producer Skeletonema costatum (algae) and the herbivorous copepod Acartia tonsa). The results suggest that the potential for acute toxicity is higher in WAFs from non-weathered oils than WAFs from the field weathered oils. The Source oils contained a large fraction of soluble and bioavailable components (such as BTEX (benzene, toluene, ethyl benzene, xylenes) and naphthalene), whereas in the surface collected oils these components were depleted by dissolution into the water column as the oil rose to the surface and by evaporative loss after reaching the sea surface.

  3. Acute Toxicity of Water-Accommodated Fraction and Chemically Enhanced WAF of Bunker C Oil and Dispersant to a Microalga Tetraselmis tetrathele.

    PubMed

    Santander-Avanceña, Sheryll S; Sadaba, Resurreccion B; Taberna, Hilario S; Tayo, Gilma T; Koyama, Jiro

    2016-01-01

    This study assessed the toxicity of water-accommodated fraction (WAF) and chemically enhanced WAF (CEWAF) of bunker C oil and dispersant (DISP) to a microalga, Tetraselmis tetrathele. The 72-h median effective concentration (72-h EC50) of CEWAF and DISP were determined at 3.30% and 2.40%, respectively. The no observed effect concentration (NOEC) of CEWAF to T. tetrathele was at 2.0% and lowest observed effect concentration (LOEC) was at 3.0% while NOEC and LOEC of DISP to T. tetrathele were determined at 1.0% and 2.0%, respectively. The addition of dispersant to oil increased the amount of total PAH present in the CEWAF test solutions. DISP alone was highly toxic, and the toxicity of CEWAF was primarily caused by the presence of dispersant.

  4. Ecdysone receptor (EcR) and ultraspiracle (USP) genes from the cyclopoid copepod Paracyclopina nana: Identification and expression in response to water accommodated fractions (WAFs).

    PubMed

    Puthumana, Jayesh; Lee, Min-Chul; Han, Jeonghoon; Kim, Hui-Su; Hwang, Dae-Sik; Lee, Jae-Seong

    2017-02-01

    Ecdysteroid hormones are pivotal in the development, growth, and molting of arthropods, and the hormone pathway is triggered by binding ecdysteroid to a heterodimer of the two nuclear receptors; ecdysone receptors (EcR) and ultraspiracle (USP). We have characterized EcR and USP genes, and their 5'-untranslated region (5'-UTR) from the copepod Paracyclopina nana, and studied mRNA transcription levels in post-embryonic stages and in response to water accommodated fractions (WAFs) of crude oil. The open reading frames (ORF) of EcR and USP were 1470 and 1287bp that encoded 490 and 429 amino acids with molecular weight of 121.18 and 105.03kDa, respectively. Also, a well conserved DNA-binding domain (DBD) and ligand-binding domain (LBD) were identified which confirmed by phylogenetic analysis. Messenger RNA transcriptional levels of EcR and USP were developmental stage-specific in early post-embryonic stages (N3-4). However, an evoked expression of USP was observed throughout copepodid stage and in adult females. WAFs (40 and 80%) were acted as an ecdysone agonist in P. nana, and elicited the mRNA transcription levels in adults. Developmental stage-specific transcriptional activation of EcR and USP in response to WAFs was observed. USP gene was down-regulated in the nauplius in response to WAF, whereas up-regulation of USP was observed in the adults. This study represents the first data of molecular elucidation of EcR and USP genes and their regulatory elements from P. nana and the developmental stage specific expression in response to WAFs, which can be used as potential biomarkers for environmental stressors with ecotoxicological evaluations in copepods.

  5. Expression of three novel cytochrome P450 (CYP) and antioxidative genes from the polychaete, Perinereis nuntia exposed to water accommodated fraction (WAF) of Iranian crude oil and benzo[a]pyrene.

    PubMed

    Won, Eun-Ji; Rhee, Jae-Sung; Shin, Kyung-Hoon; Jung, Jee-Hyun; Shim, Won Joon; Lee, Young-Mi; Lee, Jae-Seong

    2013-09-01

    To report a novel CYP genes and to evaluate its potency as a biomarker for oil pollution, we cloned three CYP genes and measured their expression profiles under controlled lab conditions using real-time reverse transcription PCR (real-time RT-PCR) after exposure of the water accommodated fraction (WAF) of Iranian crude oil and benzo[α]pyrene (B[α]P) as a positive control. Of these, CYP432A1 (CYP3 clan) gene was significantly induced by B[α]P exposure, indicating that the CYP3 clan gene would play an important role in polycyclic aromatic hydrocarbon (PAH) metabolisms, particularly for B[α]P in this species. However, the Perinereis nuntia CYP431A1 mRNA, a CYP2 clan gene, was sensitively expressed to WAF exposure with other two CYP genes. As one of Phase II detoxification enzymes, the glutathione S-transferase (GST) genes also upregulated with other antioxidant genes (SOD and CAT), indicating that WAF-exposed P. nuntia was properly responding to this kind of chemical stress. Thus, three CYP genes from the polychaete, P. nuntia have a potential as a biomarker in monitoring of the marine sediment after an oil spill accident.

  6. Toxicity of fuel oil water accommodated fractions on two marine microalgae, Skeletonema costatum and Chlorela spp.

    PubMed

    Chao, Min; Shen, Xinqiang; Lun, Fengxia; Shen, Anglv; Yuan, Qi

    2012-05-01

    In this paper, the acute toxicity of four fuel oils including F120, F180, F380 and No.-20 was evaluated by exposing the marine microalgae Chlorela spp. (Chlorophyta) and Skeletonema costatum (Bacillariophyta) in the fuel oil water accommodated fractions (WAF). The bioassay showed that F180 WAF was the most toxic to both microalgae. The 96 h EC(50) value of F180 WAF for Skeletonema costatum and Chlorela spp. was 9.41 and 13.63 mg/L expressed in concentration of total petroleum hydrocarbons, respectively. WAFs of F120, F180 and F380 were more toxic to Skeletonema costatum than to Chlorela spp. In contrast, No.-20 WAF did not show significant toxicity for both Skeletonema costatum and Chlorela spp.

  7. Toxicity of Water Accommodated Fractions of Estonian Shale Fuel Oils to Aquatic Organisms.

    PubMed

    Blinova, Irina; Kanarbik, Liina; Sihtmäe, Mariliis; Kahru, Anne

    2016-02-01

    Estonia is the worldwide leading producer of the fuel oils from the oil shale. We evaluated the ecotoxicity of water accommodated fraction (WAF) of two Estonian shale fuel oils ("VKG D" and "VKG sweet") to aquatic species belonging to different trophic levels (marine bacteria, freshwater crustaceans and aquatic plants). Artificial fresh water and natural lake water were used to prepare WAFs. "VKG sweet" (lower density) proved more toxic to aquatic species than "VKG D" (higher density). Our data indicate that though shale oils were very toxic to crustaceans, the short-term exposure of Daphnia magna to sub-lethal concentrations of shale fuel oils WAFs may increase the reproductive potential of survived organisms. The weak correlation between measured chemical parameters (C10-C40 hydrocarbons and sum of 16 PAHs) and WAF's toxicity to studied species indicates that such integrated chemical parameters are not very informative for prediction of shale fuel oils ecotoxicity.

  8. Behavior of Corophium volutator (Crustacea, Amphipoda) exposed to the water-accommodated fraction of oil in water and sediment.

    PubMed

    Kienle, Cornelia; Gerhardt, Almut

    2008-03-01

    We investigated the short-term effects of the water accommodated fraction (WAF) of weathered Forties crude oil on the behavior of Corophium volutator in the Multispecies Freshwater Biomonitor (MFB). When exposing C. volutator to 25 and 50% WAF in aqueous exposures, hyperactivity with an additional increase in ventilation was detected, whereas exposure to 100% WAF led to hypoactivity (narcosis). In a sediment exposure with 100% WAF, there was an increased tendency toward hyperactivity. In a pulse experiment, hyperactivity appeared at and after a 130-min exposure to 50% WAF in a majority of cases. Our experiments suggest that the behavior of C. volutator as measured in the MFB may be an appropriate parameter for coastal monitoring.

  9. Toxicity and phototoxicity of water-accommodated fraction obtained from Prestige fuel oil and Marine fuel oil evaluated by marine bioassays.

    PubMed

    Saco-Alvarez, Liliana; Bellas, Juan; Nieto, Oscar; Bayona, Josep María; Albaigés, Joan; Beiras, Ricardo

    2008-05-15

    Acute toxicity and phototoxicity of heavy fuel oil extracted directly from the sunken tanker Prestige in comparison to a standard Marine fuel oil were evaluated by obtaining the water-accommodated fraction (WAF) and using mussel Mytilus galloprovincialis and sea urchin Paracentrotus lividus embryogenesis bioassays, and copepod Acartia tonsa and fish Cyprinodon variegatus survival bioassays. Aromatic hydrocarbon (AH) levels in WAF were measured by gas chromatography. Prestige WAF was not phototoxic, its median effective concentrations (EC50) were 13% and 10% WAF for mussel and sea urchin respectively, and maximum lethal threshold concentrations (MLTC) were 12% and 50% for copepod and fish respectively. Marine WAF resulted phototoxic for mussel bioassay. EC50s of Marine WAF were 50% for sea urchin in both treatments and 20% for mussel under illumination. Undiluted Marine WAF only caused a 20% decrease in mussel normal larvae. Similar sensitivities were found among sea urchins, mussels and copepods, whilst fish were less sensitive. Unlike Marine WAF, Prestige WAF showed EC50 values at dilutions below 20%, and its toxicity was independent of lighting conditions. The differences in toxicity between both kinds of fuel could not be explained on the basis of total AH content.

  10. Effect of water accommodated fraction of 0# diesel oil and crude oil on EROD activity of liver of Sparus macrocephlus and its mRNA expression.

    PubMed

    Lei, Li; Shen, Xinqiang; Jiang, Mei

    2016-12-01

    We studied the effect of water accommodated fractions (WAF) of 0# diesel and crude oil on ethoxy resorufin o-deethylase (EROD) activity and CYP1A1 mRNA expression quantity in the liver of Sparus macrocephlus. We found that there were some differences in the EROD activity and CYP1A1 mRNA induction between these two petroleum hydrocarbons. Both the EROD activity and CYP1A1 mRNA expression of fish exposed to 0# diesel WAF were higher than those of crude oil WAF fish. The EROD activities and CYP1A1 mRNA expressions in the livers 0# diesel WAF exposed group declined faster than those of crude oil WAF and the recovery of EROD activity and CYP1A1 mRNA expression in the crude oil group was higher than that of 0# diesel group.

  11. Integrated biomarker response index used in laboratory exposure of the mussel Mytilus edulis to water accommodated fractions of crude oil

    NASA Astrophysics Data System (ADS)

    Yuan, Mengqi; Wang, You; Zhang, Xinxin; Hu, Shunxin; Tang, Xuexi

    2017-03-01

    Oil pollution is a serious environmental problem in coastal areas and to set up a quantitative evaluation method in monitoring the environmental pollution is of great importance. Individuals of Mytilus edulis, collected from the coastal area of Qingdao, were thus exposed to the water accommodated fractions (WAFs) of crude oil under controlled lab conditions. Mussels were exposed to different concentrations of WAFs for 96h acute toxicity experiment and 15d chronic toxicity experiment, a 7d restoration experiment was conducted after toxicity experiment as well. The activities of the antioxidant enzymes (CAT, GST, GPx and GR) were measured in gills of mussels. The purpose of the study was to screen out a series of sensitive biomarkers and set up an Integrated Biomarker Response index (IBR) for marine oil pollution monitoring. Results showed: (1) WAFs exposure induced the increased activities of CAT, GST, GPx and GR in gills, revealing a stimulation effect on the antioxidant system. Activities of enzymes were more significantly altered in response to higher concentrations of WAFs (2.3 and 5.0 mg.L-1) than lower concentrations (0.5 and 1.1 mg.L-1). After restoration experiment, the activities decreased to the initial levels. (2) IBR was set up base on the laboratory experiment, which showed a positive relationship with WAFs exposure concentrations. Therefore, IBR was suggested to be a potentially useful tool in assessing the oil pollution.

  12. Reproductive effects of the water-accommodated fraction of a natural gas condensate in the Indo-Pacific reef-building coral Pocillopora damicornis.

    PubMed

    Villanueva, R D; Yap, H T; Montaño, M N E

    2011-11-01

    Toxic effects of the water-accommodated fraction (WAF) of a natural gas condensate on the reproduction of the brooding coral Pocillopora damicornis were studied in short-term (24 h) laboratory experiments. Coral fragments were exposed to varying concentrations of condensate WAF during different reproductive phases: gametogenesis, early embryogenesis, and late embryogenesis (when nighttime planulation occurs). During gametogenesis, exposure to condensate WAF did not inhibit subsequent production of larvae. On the other hand, exposure to >25% WAF of gravid corals, at early and late embryogenesis, resulted in abortion and early release of larvae, respectively, with higher percentages of larvae expelled in fragments treated with higher concentrations of condensate WAF at least 3h after onset of exposure. Aborted larvae during early embryogenesis were 'premature', as they are of small size (0.06±0.03 mm³), low metamorphic competency (54%), and white in coloration, with a pale brown oral end (indicating low density of zooxanthellae). Those larvae released at the latter part of embryogenesis are bigger in size (0.22±0.08 mm³), possess 100% metamorphic competency, and are brown in coloration (high density of zooxanthellae). Aside from direct effects on reproduction, fragment mortality index was higher in samples exposed to higher concentrations of condensate WAF (>25%), hence lowering the number of potentially reproducing polyps. Altogether, exposure to >25% natural gas condensate WAF for at least 3h can potentially disrupt the replenishment of coral populations due to negative effects on reproduction and early life processes.

  13. Sensitivity and Antioxidant Response of Chlorella sp. MM3 to Used Engine Oil and Its Water Accommodated Fraction.

    PubMed

    Ramadass, Kavitha; Megharaj, Mallavarapu; Venkateswarlu, Kadiyala; Naidu, Ravi

    2016-07-01

    We exposed the microalgal strain, Chlorella sp. MM3, to unused or used engine oil, or their water accommodated fractions (WAFs) to determine growth inhibition and response of antioxidant enzymes. Oil type and oil concentration greatly affected the microalgal growth. Used oil at 0.04 % (0.4 g L(-1)) resulted in 50 % inhibition in algal growth, measured in terms of chlorophyll-a, while the corresponding concentration of unused oil was nontoxic. Similarly, used oil WAF showed significant toxicity to the algal growth at 10 % level, whereas WAF from unused oil was nontoxic even at 100 % concentration. Peroxidase enzyme in the microalga significantly increased with used oil at concentrations above 0.04 g L(-1) whereas the induction of superoxide dismutase and catalase was apparent only at 0.06 g L(-1). Activities of the antioxidant enzymes increased significantly when the microalga was exposed to 75 and 100 % WAF obtained from used oil. The used oil toxicity on microalga could be due to the presence of toxic soluble mono- and polyaromatic compounds, heavy metals, and other compounds attained by the oil during its use in the motor engines.

  14. Comparative effects of the water accommodated fraction of three oils on mussels. 2. Quantitative alterations in the structure of the digestive tubules.

    PubMed

    Cajaraville, M P; Marigómez, J A; Díez, G; Angulo, E

    1992-05-01

    1. Mussels have been exposed to the water accommodated fraction (WAF) of Ural and Maya crude oils and of a lubricant oil for 91 days and the digestive gland structure has been studied (a) by planimetry, to calculate the mean epithelial thickness (MET), the mean diverticular radius (MDR) and the mean luminal radius (MLR) of the digestive tubules and (b) by subjective tubule grading, to determine the relative (%) occurrence of the different tubule types found in the digestive gland (holding, absorpting, disintegrating, reconstituting and necrotic tubules). 2. Exposure to the 3 types of WAF leads to lowered MET and MET/MDR values, and concomitant higher MLR/MET values. In the case of lubricant- and Ural-WAF exposures, MDR decreases significantly at increasing exposure-doses. 3. Exposure to oil WAFs leads to decreased percentages of holding and absorpting tubules. This decrease is concomitant with an increase of disintegrating tubules in lubricant- and Ural-WAF exposures, and with an increase of regenerating tubules in the Maya-WAF exposure. 4. The statistical significance of the differences found between control and exposed mussels is better at Day 21 than at any other further sampling day (35, 77 and 91). 5. Multiple correlation coefficients between all the percentages of tubule types and each planimetric parameter present an excellent statistical significance.

  15. Risk assessment of the National Institute of Standards and Technology petroleum crude oil standard water accommodated fraction: further application of a copepod-based, full life-cycle bioassay.

    PubMed

    Bejarano, Adriana C; Chandler, G Thomas; He, Lijian; Cary, Tawnya L; Ferry, John L

    2006-07-01

    The U.S. National Institute of Standards and Technology (NIST) petroleum crude oil was used to generate NIST water-accommodated hydrocarbon fractions (WAFs) for standardized assessment of crude oil effects on the copepod Amphiascus tenuiremis. Effects were assessed using a 96-well microplate, full life-cycle test. Briefly, nauplii (age, 24 h) were reared individually to adults (n > or =120 nauplii/treatment) in microplate wells containing 200 microl of treatment solution (seawater control [0%] or 10, 30, 50, or 100% NIST-WAF). Nauplii were monitored through development to adulthood, and mature virgin male:female pairs mated in wells containing original treatments (<30 d). A second bioassay using 0, 10, 30, and 50% WAFs (n > or =60 nauplii/treatment) was conducted to assess the effects of ultraviolet (UV) light on naupliar endpoints (<16 d). In the first experiment, nauplius-to-copepodite survival in exposures to 100% WAF was 27% +/- 6% lower than in controls (92% +/- 1%), but copepodite-to-adult survival was greater than 90% across all treatments. Analysis of development curves showed that nauplii in the 10% WAF developed into copepodites 25% faster, whereas nauplii in the 50 and 100% WAFs developed 17% slower, than controls. Copepodite development into male and female copepods was significantly delayed (2 and 4 d, respectively) in the 100% WAF compared to controls. Although none of the WAF exposures had significant effects on fertilization success or total viable production (p > 0.05), embryo hatching in the 100% WAF was significantly less (70.0% +/- 21.2%) than that in controls (87.0% +/- 19.4%). Results from the UV bioassay showed that relatively short exposures (<14 d) to 30 and 50% WAFs in the presence of UV light caused negative effects on copepod survival and development. Naupliar-stage survival and developmental endpoints were the most sensitive indicators of exposure to the NIST crude oil WAF

  16. Thyroid Hormone Disruption by Water-Accommodated Fractions of Crude Oil and Sediments Affected by the Hebei Spirit Oil Spill in Zebrafish and GH3 Cells.

    PubMed

    Kim, Sujin; Sohn, Ju Hae; Ha, Sung Yong; Kang, Habyeong; Yim, Un Hyuk; Shim, Won Joon; Khim, Jong Seong; Jung, Dawoon; Choi, Kyungho

    2016-06-07

    A crude oil and the coastal sediments that were affected by the Hebei Spirit Oil Spill (HSOS) of Taean, Korea were investigated for thyroid hormone disruption potentials. Water-accommodated fractions (WAFs) of Iranian Heavy crude oil, the major oil type of HSOS, and the porewater or leachate of sediment samples collected along the coast line of Taean were tested for thyroid disruption using developing zebrafish and/or rat pituitary GH3 cells. Major polycyclic aromatic hydrocarbons (PAHs) and their alkylated forms were also measured from the test samples. In zebrafish larvae, significant decreases in whole-body thyroxine (T4) and triiodothyronine (T3) levels, along with transcriptional changes of thyroid regulating genes, were observed following 5 day exposure to WAFs. In GH3 cells, transcriptions of thyroid regulating genes were influenced following the exposure to the sediment samples, but the pattern of the regulatory change was different from those observed from the WAFs. Composition of PAHs and their alkylated homologues in the WAFs could partly explain this difference. Our results clearly demonstrate that WAFs of crude oil can disrupt thyroid function of larval zebrafish. Sediment samples also showed thyroid disrupting potentials in the GH3 cell, even several years after the oil spill. Long-term ecosystem consequences of thyroid hormone disruption due to oil spill deserve further investigation.

  17. Characterization of oil and water accommodated fractions used to conduct aquatic toxicity testing in support of the Deepwater Horizon oil spill natural resource damage assessment.

    PubMed

    Forth, Heather P; Mitchelmore, Carys L; Morris, Jeffrey M; Lipton, Joshua

    2016-11-02

    The Deepwater Horizon blowout resulted in the release of millions of barrels of crude oil. As part of the Trustees' Natural Resource Damage Assessment, a testing program was implemented to evaluate the toxicity of Deepwater Horizon oil and oil/dispersant mixtures to aquatic organisms from the Gulf of Mexico. Because of the variety of exposures that likely occurred, the program included 4 Deepwater Horizon oils, which encompassed a range of weathering states, and 3 different oil-in-water mixing methods, for a total of 12 unique water accommodated fractions (WAFs). The present study reports on the chemical characteristics of these 4 Deepwater Horizon oils and 12 WAFs. In addition, to better understand exposure chemistry, an examination was conducted of the effects of WAF preparation parameters-including mixing energy, starting oil composition, and oil-to-water mixing ratios-on the chemical profiles and final concentrations of these 12 WAFs. The results showed that the more weathered the starting oil, the lower the concentrations of the oil constituents in the WAF, with a shift in composition to the less soluble compounds. In addition, higher mixing energies increased the presence of insoluble oil constituents. Finally, at low to mid oil-to-water mixing ratios, the concentration and composition of the WAFs changed with changing mixing ratios; this change was not observed at higher mixing ratios (i.e., >1 g oil/L). Ultimately, the present study provides a basic characterization of the oils and WAFs used in the testing program, which helps to support interpretation of the more than 500 Deepwater Horizon Natural Resource Damage Assessment toxicity testing results and to enable a comparison of these results with different tests and with the field. Environ Toxicol Chem 2016;9999:1-10. © 2016 SETAC.

  18. Acute toxicity of crude oil water accommodated fraction on marine copepods: the relative importance of acclimatization temperature and body size.

    PubMed

    Jiang, Zhibing; Huang, Yijun; Chen, Quanzhen; Zeng, Jiangning; Xu, Xiaoqun

    2012-10-01

    Recent oil spillage accidents around the world greatly increase harmful risks to marine ecology. This study evaluated the influences of petroleum water accommodated fraction (WAF) on 15 typical species of marine copepods collected from a subtropical bay in East China Sea at different seasons. Copepods showed impaired swimming ability, restlessness, loss of balance, anoxic coma, and even death when they were acutely exposed to the crude oil WAF under laboratory conditions. The LC(50) values (expressed in total petroleum hydrocarbon concentration) indicated that the tolerances of copepods to WAF decreased significantly (P < 0.05) with increased exposure duration and natural water temperatures (acclimatization temperature). The sensitivity of the copepods was species-specific (P < 0.01), and there was a significant (P < 0.05) positive correlation between the 48-h LC(50) and body size. Therefore, the small copepod species confront more survival challenges under oil contamination stress, especially in the warm months or regions.

  19. Identification of the Full 46 Cytochrome P450 (CYP) Complement and Modulation of CYP Expression in Response to Water-Accommodated Fractions of Crude Oil in the Cyclopoid Copepod Paracyclopina nana.

    PubMed

    Han, Jeonghoon; Won, Eun-Ji; Kim, Hui-Su; Nelson, David R; Lee, Su-Jae; Park, Heum Gi; Lee, Jae-Seong

    2015-06-02

    The 46 cytochrome P450 (CYP) gene superfamily was identified in the marine copepod Paracyclopina nana after searching an RNA-seq database and comparing it with other copepod CYP gene families. To annotate the 46 Pn-CYP genes, a phylogenetic analysis of CYP genes was performed using a Bayesian method. Pn-CYP genes were separated into five different clans: CYP2, CYP3, CYP20, CYP26, and mitochondrial. Among these, the principal Pn-CYP genes involved in detoxification were identified by comparing them with those of the copepod Tigriopus japonicus and were examined with respect to their responses to exposure to a water-accommodated fraction (WAF) of crude oil and to the alkylated forms of two polycyclic aromatic hydrocarbons (PAHs; phenanthrene and fluorene). The expression of two Pn-CYP3027 genes (CYP3027F1 and CYP3027F2) was increased in response to WAF exposure and also was upregulated in response to the two alkylated PAHs. In particular, Pn-CYP3027F2 showed the most notable increase in response to 80% WAF exposure. These two responsive CYP genes (Pn-CYP3027F1 and CYP3027F2) were also phylogenetically clustered into the same clade of the WAF- and alkylated PAH-specific CYP genes of the copepod T. japonicus, suggesting that these CYP genes would be those chiefly involved in detoxification in response to WAF exposure in copepods. In this paper, we provide information on the copepod P. nana CYP gene superfamily and also speculate on its potential role in the detoxification of PAHs in marine copepods. Despite the nonlethality of WAF, Pn-CYP3027F2 was rapidly and significantly upregulated in response to WAF that may serve as a useful biomarker of 40% or higher concentration of WAF exposure. This paper will be helpful to better understand the molecular mechanistic events underlying the metabolism of environmental toxicants in copepods.

  20. An evaluation of the endocrine disruptive potential of crude oil water accommodated fractions and crude oil contaminated surface water to freshwater organisms using in vitro and in vivo approaches.

    PubMed

    Truter, J Christoff; van Wyk, Johannes H; Oberholster, Paul J; Botha, Anna-Maria; Mokwena, Lucky M

    2016-10-27

    Knowledge regarding the potential impacts of crude oil on endocrine signaling in freshwater aquatic vertebrates is limited. The expression of selected genes as biomarkers for altered endocrine signaling was studied in African clawed frog, Xenopus laevis, tadpoles and juvenile Mozambique tilapia, Oreochromis mossambicus, exposed to weathered bunker and unweathered refinery crude oil water accommodated fractions (WAFs). In addition, the expression of the aforementioned genes was quantified in X. laevis tadpoles exposed to surface water collected from the proximity of an underground oil bunker. The (anti)estrogenicity and (anti)androgenicity of crude oil, crude oil WAFs, and surface water were furthermore evaluated using recombinant yeast. Thyroid hormone receptor beta expression was significantly down-regulated in X. laevis in response to both oil WAF types, whereas a further thyroid linked gene, type 2 deiodinase, was up-regulated in O. mossambicus exposed to a high concentration of bunker oil WAF. In addition, both WAFs altered the expression of the adipogenesis-linked peroxisome proliferator-activated receptor gamma in X. laevis. The crude oil and WAFs exhibited antiestrogenic and antiandrogenic activity in vitro. However, O. mossambicus androgen receptor 2 was the only gene, representing the reproductive system, significantly affected by WAF exposure. Estrogenicity, antiestrogenicity, and antiandrogenicity were detected in surface water samples; however, no significant changes were observed in the expression of any of the genes evaluated in X. laevis exposed to surface water. The responses varied among the 2 model organisms used, as well as among the 2 types of crude oil. Nonetheless, the data provide evidence that crude oil pollution may lead to adverse health effects in freshwater fish and amphibians as a result of altered endocrine signaling. Environ Toxicol Chem 2016;9999:1-13. © 2016 SETAC.

  1. Comparative study on acute effects of water accommodated fractions of an artificially weathered crude oil on Calanus finmarchicus and Calanus glacialis (Crustacea: Copepoda).

    PubMed

    Hansen, Bjørn Henrik; Altin, Dag; Rørvik, Siv F; Øverjordet, Ida Beathe; Olsen, Anders J; Nordtug, Trond

    2011-01-15

    Extrapolation of ecotoxicological data from temperate species for use in risk assessment in the polar environments may be difficult since polar organisms as a rule differ from temperate species in terms of life span length, developmental time, surface-to-volume ratios, metabolic rates, total energy usage and lipid content for energy storage. In the current work we performed a comparative study where two closely related and morphologically similar copepod species, Calanus finmarchicus (temperate-boreal) and Calanus glacialis (arctic), were exposed to water accommodated fractions (WAF) of oil in a series of parallel experiments. The two species, adapted to 10°C and 2°C, respectively, were compared on the basis of acute ecotoxicity (LC(50)) and the WAF-mediated induction of the gene encoding glutathione S-transferase (GST). In addition, an experiment was conducted in order to reveal relationships between lipid content and acute toxicity. LC(50) values differed between the two species, and the Arctic copepod appeared less sensitive than the temperate-boreal species. The lipid contents of the two species, measured biometrically, were comparable, and the relationships between lipid content and response (reduced survival) to acute WAF exposure followed the same trend: Lipid-rich copepods survived longer than lipid-poor copepods at the same exposure concentration. In terms of GST expression, both species showed concentration-dependent and exposure time-dependent trends. However, as for the acute toxicity data, the Arctic copepod appeared to respond slower and with a lower intensity. From the study it can be concluded that temperature and lipid content are important factors for assessing differences between temperate and Arctic species, and that a delayed response in organisms adapted to low temperatures needs to be corrected for when extrapolating toxicity data from species with other temperature optimums for use in Arctic environments.

  2. Effect of dispersant on the composition of the water-accommodated fraction of crude oil and its toxicity to larval marine fish.

    PubMed

    Couillard, Catherine M; Lee, Kenneth; Légaré, Benoît; King, Thomas L

    2005-06-01

    Newly hatched mummichog (Fundulus heteroclitus) were exposed in a 96-h static renewal assay to water-accommodated fractions of dispersed crude oil (DWAF) or crude oil (WAF) to evaluate if the dispersant-induced changes in aqueous concentrations of polycyclic aromatic hydrocarbons (PAH) affected larval survival, body length, or ethoxyresorufin-O-deethylase (EROD) activity. Weathered Mesa light crude oil (0.05-1 g/L) and filtered seawater with or without the addition of Corexit 9500 were used to prepare DWAF and WAE At 0.2 g/L, the addition of dispersant caused a two- and fivefold increase in the concentrations of total PAH (sigmaPAH) and high-molecular-weight PAH (HMWPAH) with three or more benzene rings. Highest mortality rates (89%) were observed in larvae exposed to DWAF (0.5 g/L; sigmaPAH, 479 ng/ml). A reduction in body length was correlated with increased levels of sigmaPAH (r2 = 0.65, p = 0.02) and not with HMWPAH. The EROD activity increased linearly with HMWPAH (r2 = 0.99, p = 0.001) and not with sigmaPAH. Thus, chemical dispersion increased both the sigmaPAH concentrations and the proportion of HMWPAH in WAF. Dispersed HMWPAH were bioavailable, as indicated by a significantly increased EROD activity in exposed mummichog larvae, and this may represent a significant hazard for larval fish.

  3. WAFs lead molting retardation of naupliar stages with down-regulated expression profiles of chitin metabolic pathway and related genes in the copepod Tigriopus japonicus.

    PubMed

    Hwang, Dae-Sik; Lee, Min-Chul; Kyung, Do-Hyun; Kim, Hui-Su; Han, Jeonghoon; Kim, Il-Chan; Puthumana, Jayesh; Lee, Jae-Seong

    2017-03-01

    Oil pollution is considered being disastrous to marine organisms and ecosystems. As molting is critical in the developmental process of arthropods in general and copepods, in particular, the impact will be adverse if the target of spilled oil is on molting. Thus, we investigated the harmful effects of water accommodated fractions (WAFs) of crude oil with an emphasis on inhibition of chitin metabolic pathways related genes and developmental retardation in the copepod Tigriopus japonicus. Also, we analysed the ontology and domain of chitin metabolic pathway genes and mRNA expression patterns of developmental stage-specific genes. Further, the developmental retardation followed by transcriptional modulations in nuclear receptor genes (NR) and chitin metabolic pathway-related genes were observed in the WAFs-exposed T. japonicus. As a result, the developmental time was found significantly (P<0.05) delayed in response to 40% WAFs in comparison with that of control. Moreover, the NR gene, HR3 and chitinases (CHT9 and CHT10) were up-regulated in N4-5 stages, while chitin synthase genes (CHS-1, CHS-2-1, and CHS-2-2) down-regulated in response to WAFs. In brief, a high concentration of WAFs repressed nuclear receptor genes but elicited activation of some of the transcription factors at low concentration of WAFs, resulting in suppression of chitin synthesis. Thus, we suggest that WAF can lead molting retardation of naupliar stages in T. japonicus through down-regulations of chitin metabolism. These findings will provide a better understanding of the mode of action of chitin biosynthesis associated with molting mechanism in WAF-exposed T. japonicus.

  4. Fast preparation of the seawater accommodated fraction of heavy fuel oil by sonication.

    PubMed

    Elordui-Zapatarietxe, Saioa; Albaigés, Joan; Rosell-Melé, Antoni

    2008-12-01

    The seawater accommodated fraction (SWAF) of oil is widely used for the assessment of its toxicity. However, its preparation in the laboratory is time consuming, and results from different authors are difficult to compare as preparation methods vary. Here we describe a simple and fast set up, using sonication, to produce reproducible SWAF in the laboratory. The system was tested on heavy fuel oil placed on seawater at different salinity and temperature conditions. Maximum dissolution of the oil was achieved after 24h, independently of both seawater salinity and temperature. Our findings are discussed in relation to the fate of the oil from the deep spill of the Prestige tanker. Changes in temperature in the open ocean are bound to have larger impact in the concentration of the SWAF than the corresponding values of sea water salinity. We anticipate that in this type of incident the highest SWAF, as the oil reaches the sea surface, should be expected in the warmest and less saline waters of the water column.

  5. Chemical Analysis of Water-accommodated Fractions of Crude Oil Spills Using TIMS-FT-ICR MS.

    PubMed

    Benigni, Paolo; Marin, Rebecca; Sandoval, Kathia; Gardinali, Piero; Fernandez-Lima, Francisco

    2017-03-03

    Multiple chemical processes control how crude oil is incorporated into seawater and also the chemical reactions that occur overtime. Studying this system requires the careful preparation of the sample in order to accurately replicate the natural formation of the water-accommodated fraction that occurs in nature. Low-energy water-accommodated fractions (LEWAF) are carefully prepared by mixing crude oil and water at a set ratio. Aspirator bottles are then irradiated, and at set time points, the water is sampled and extracted using standard techniques. A second challenge is the representative characterization of the sample, which must take into consideration the chemical changes that occur over time. A targeted analysis of the aromatic fraction of the LEWAF can be performed using an atmospheric-pressure laser ionization source coupled to a custom-built trapped ion mobility spectrometry-Fourier transform-ion cyclotron resonance mass spectrometer (TIMS-FT-ICR MS). The TIMS-FT-ICR MS analysis provides high-resolution ion mobility and ultrahigh-resolution MS analysis, which further allow the identification of isomeric components by their collision cross-sections (CCS) and chemical formula. Results show that as the oil-water mixture is exposed to light, there is significant photo-solubilization of the surface oil into the water. Over time, the chemical transformation of the solubilized molecules takes place, with a decrease in the number of identifications of nitrogen- and sulfur-bearing species in favor of those with a greater oxygen content than were typically observed in the base oil.

  6. Evaluating toxicity of heavy fuel oil fractions using complementary modeling and biomimetic extraction methods.

    PubMed

    Redman, Aaron D; Parkerton, Thomas F; Letinski, Daniel J; Manning, Ryan G; Adams, Julie E; Hodson, Peter V

    2014-09-01

    The toxicity of chemically dispersed heavy fuel oil (HFO) and 3 distillate fractions to rainbow trout (Oncorhynchus mykiss) embryos was evaluated using the PETROTOX model and a biomimetic extraction technique that involved passive sampling of oil-contaminated test media with solid-phase microextraction (SPME) fibers. Test solutions for toxicity testing were generated using a combination of dispersant and high-energy mixing. The resulting water accommodated fractions (WAF) provided complex exposure regimens that included both dissolved hydrocarbons and oil droplets. The toxicity of the various fractions differed by approximately 3 orders of magnitude when expressed on the basis of WAF dilution. Using detailed compositional data, the PETROTOX model predicted the speciation of hydrocarbons between dissolved and oil droplet phases and explained observed toxicity based on computed dissolved phase toxic units (TUs). A key finding from model calculations was that dissolved hydrocarbon exposures and associated TUs were a nonlinear function of WAF dilution, because dissolved hydrocarbons were largely controlled by the dissolution of oil droplets that were transferred in WAF dilutions. Hence, oil droplets served to "buffer" dissolved concentrations in WAF dilutions at loadings greater than 1 mg/L, resulting in higher dissolved concentrations and TUs than expected based on dilution. The TUs computed at each WAF dilution explained the observed toxicity among the HFO and fractions to within a factor of 3. Dissolved material measured by SPME showed a consistent relationship with model-predicted TUs, confirming the utility of this approach for providing an integrated measure of exposure to bioavailable hydrocarbons. These 2 approaches provide complementary tools for better defining bioavailability of complex petroleum substance.

  7. Suppression of p53 and p21CIP1/WAF1 reduces arsenite-induced aneuploidy

    PubMed Central

    Salazar, Ana María; Miller, Heather L.; McNeely, Samuel C.; Sordo, Monserrat; Ostrosky-Wegman, Patricia; States, J. Christopher

    2009-01-01

    Aneuploidy and extensive chromosomal rearrangements are common in human tumors. The role of DNA damage response proteins p53 and p21CIP1/WAF1 in aneugenesis and clastogenesis was investigated in telomerase immortalized diploid human fibroblasts using siRNA suppression of p53 and p21CIP1/WAF1. Cells were exposed to the environmental carcinogen sodium arsenite (15 and 20 µM), and the induction of micronuclei (MN) was evaluated in binucleated cells using the cytokinesis-block assay. To determine whether MN resulted from missegregation of chromosomes or from chromosomal fragments, we used a fluorescent in situ hybridization with a centromeric DNA probe. Micronuclei were predominantly of clastogenic origin in control cells regardless of p53 or p21CIP1/WAF1 expression. MN with centromere signals in cells transfected with NSC siRNA or Mock increased 30% after arsenite exposure, indicating that arsenite induced aneuploidy in the tGM24 cells. Although suppression of p53 increased the fraction of arsenite-treated cells with MN, it caused a decrease in the fraction of with centeromeric DNA. Suppression of p21CIP1/WAF1 like p53 suppression decreased the fraction of with centromeric DNA. Our results suggest that cells lacking normal p53 function cannot become aneuploid because they die by mitotic arrest-associated apoptosis, whereas cells with normal p53 function that are able to exit from mitotic arrest can become aneuploid. Furthermore our current results support this role for p21CIP1/WAF1. Since suppression of p21CIP1/WAF1 caused a decrease in aneuploidy induced by arsenite suggesting that p21CIP1/WAF1 plays a role in mitotic exit. PMID:20000476

  8. p21WAF1 modulates drug-induced apoptosis and cell cycle arrest in B-cell precursor acute lymphoblastic leukemia

    PubMed Central

    Davies, Carwyn; Hogarth, Linda A; Mackenzie, Karen L; Hall, Andrew G; Lock, Richard B

    2015-01-01

    p21WAF1 is a well-characterized mediator of cell cycle arrest and may also modulate chemotherapy-induced cell death. The role of p21WAF1 in drug-induced cell cycle arrest and apoptosis of acute lymphoblastic leukemia (ALL) cells was investigated using p53-functional patient-derived xenografts (PDXs), in which p21WAF1 was epigenetically silenced in T-cell ALL (T-ALL), but not in B-cell precursor (BCP)-ALL PDXs. Upon exposure to diverse cytotoxic drugs, T-ALL PDX cells exhibited markedly increased caspase-3/7 activity and phosphatidylserine (PS) externalization on the plasma membrane compared with BCP-ALL cells. Despite dramatic differences in apoptotic characteristics between T-ALL and BCP-ALL PDXs, both ALL subtypes exhibited similar cell death kinetics and were equally sensitive to p53-inducing drugs in vitro, although T-ALL PDXs were significantly more sensitive to the histone deacetylase inhibitor vorinostat. Transient siRNA suppression of p21WAF1 in the BCP-ALL 697 cell line resulted in a moderate depletion of the cell fraction in G1 phase and marked increase in PS externalization following exposure to etoposide. Furthermore, stable lentiviral p21WAF1 silencing in the BCP-ALL Nalm-6 cell line accelerated PS externalization and cell death following exposure to etoposide and vorinostat, supporting previous findings. Finally, the Sp1 inhibitor, terameprocol, inhibited p21WAF1 expression in Nalm-6 cells exposed to vorinostat and also partially augmented vorinostat-induced cell death. Taken together, these findings demonstrate that p21WAF1 regulates the early stages of drug-induced apoptosis in ALL cells and significantly modulates their sensitivity to vorinostat. PMID:26506264

  9. Assessing the identity and expression level of the cytochrome P450 20A1 (CYP20A1) gene in the BPA-, BDE-47, and WAF-exposed copepods Tigriopus japonicus and Paracyclopina nana.

    PubMed

    Han, Jeonghoon; Kim, Duck-Hyun; Seo, Jung Soo; Kim, Il-Chan; Nelson, David R; Puthumana, Jayesh; Lee, Jae-Seong

    2017-03-01

    CYP20A1 is a member of the cytochrome P450 (CYP) superfamily, identified as an orphan P450 without any assigned biological function; hence, its continued status as an "orphan" gene. In order to address this shortcoming in our understanding of this superfamily, we sought to characterize the CYP20A1 gene in the copepods Tigriopus japonicus (Tj-CYP20A1) and Paracyclopina nana (Pn-CYP20A1) at their mRNA transcriptional level. We assessed the response of this gene's expression in various developmental stages and in response to treatment with bisphenol A (BPA), 2, 2', 4, 4'-tetrabromodiphenyl ether (BDE-47), and water accommodated fractions (WAFs) of crude oil. As shown in the vertebrate CYP20A1, both Tj-CYP20A1 and Pn-CYP20A1 contained characteristic conserved motifs and domain regions (I helix, K helix and heme-binding motifs) with unusual amino acid sequences apparent in their gene structure. Also molecular characterization of the putative responsive elements in the promoter regions was performed. We observed transcriptional up-regulation of these genes during post-embryonic developmental stages including sex-specific up-regulation in adults. In addition, concentration- and time-dependent mRNA transcripts in response to xenobiotics (BPA, BDE-47, and WAFs) were seen. This study focuses on the molecular elucidation of CYP20A1 genes and their interactions with xenobiotics in the copepods T. japonicus and P. nana that provides important insight into the biological importance of CYP20A1 in invertebrates.

  10. Noise coupling between accommodation and accommodative vergence

    NASA Technical Reports Server (NTRS)

    Wilson, D.

    1973-01-01

    For monocular viewing, the fluctuations in accommodative lens power in the frequency range from 0.5 to 3 Hz were found to be considerably greater than those in accommodative vergence movements of the covered eye. Considering the close synkinesis between these motor responses for step changes or slow variations in accommodative stimulus, this finding is unexpected. This apparent lack of synkinesis is found to result mainly from the fact that the decrease in small-signal linear gain with increasing frequency is more rapid in the case of the accommodative vergence system than in the case of the accommodation system, rather than from some nonlinear phenomenon.

  11. Phage-peptide display identifies the interferon-responsive, death-activated protein kinase family as a novel modifier of MDM2 and p21WAF1.

    PubMed

    Burch, Lindsay R; Scott, Mary; Pohler, Elizabeth; Meek, David; Hupp, Ted

    2004-03-12

    Phage-peptide display is a versatile tool for identifying novel protein-protein interfaces. Our previous work highlighted the selection of phage-peptides that bind to specific isoforms of MDM2 protein and in this work we subjected the putative MDM2-binding proteins to phage-peptide display to expand further on putative protein interaction maps. One peptide that bound MDM2 had significant homology to members of the death-activated protein kinase (DAPK) family, an enzyme family of no known direct link to the p53 pathway. We examined whether a nuclear member of the DAPK family named DAPK3 or ZIP kinase had direct links to the p53 pathway. ZIP kinase was cloned, purified, and the enzyme was able to phosphorylate MDM2 at Ser166, a site previously reported to be modified by Akt kinase, thus demonstrating that ZIP kinase is a bona fide MDM2-binding protein. Native ZIP kinase fractions were then subjected to phage-peptide display and one ZIP kinase consensus peptide motif was identified in p21(WAF1). ZIP kinase phosphorylates p21(WAF1) at Thr145 and alanine-substituted mutations in the p21(WAF1) phosphorylation site alter its ability to be phosphorylated by ZIP kinase. Thus, although ZIP kinase consensus sites were then defined as containing a minimal RKKx(T/S) consensus motif, alternate contacts in ZIP kinase binding are implicated, since amino acid residues surrounding the phospho-acceptor site can effect the specific activity of the kinase. Transfected ZIPK can promote the phosphorylation of p21(WAF1) at Thr145 in vivo and can increase the half-life of p21(WAF1), while the half-life of p21(WAF1[T145A]) is not effected by ZIP kinase. Thus, phage-peptide display identified an interferon-responsive protein kinase family as a novel modifier of two components of the p53 pathway, MDM2 and p21(WAF1), and underscores the utility of phage-peptide display for gaining novel insights into biochemical pathways.

  12. Handbook of Reasonable Accommodation.

    ERIC Educational Resources Information Center

    Heaton, Sandra M.; And Others

    The booklet discusses a basic concept in affirmative action and nondiscrimination for the handicapped, which requires federal agencies to make reasonable accommodation to the physical or mental limitations of a qualified handicapped applicant or employee unless the accommodation would impose an undue hardship on the agency. Reasonable…

  13. Analyzing Disability Accommodation Statements

    ERIC Educational Resources Information Center

    Barnard-Brak, L.; Lan, W. Y.

    2011-01-01

    Studies have indicated that the willingness of faculty members to accommodate students with disabilities differs according to academic discipline and instructor gender. The authors examined the attitudes of faculty members toward students with disabilities as reflected in course syllabi to discern instructor willingness to accommodate these…

  14. Habitability sleep accommodations

    NASA Technical Reports Server (NTRS)

    Fisher, H. T.

    1985-01-01

    Schematic outlines are presented with various design requirements for the accommodation of the spacecrew of Space Stations. The primary concern is for sleeping accommodations. Some other general requirements given are for a rest place, entertainment, dressing area, personal item stowage, body restraint, total privacy, external viewing, and grooming provisions. Several plans are given for sleep quarters concepts.

  15. p21WAF1 expression during spermatogenesis of the normal and X-irradiated rat.

    PubMed

    West, A; Lähdetie, J

    1997-03-01

    The cyclin dependent kinase inhibitor p21WAF1 has been shown to be upregulated during differentiation and after DNA damage in somatic cells. We examined the expression of p21WAF1 mRNA during the differentiation of germ cells in normal and X-irradiated rat testis by in situ hybridization and Northern blotting. p21WAF1 was normally expressed in primary spermatocytes of the pachytene phase, but could also be detected in round spermatids. In preparations of defined segments of the seminiferous tubules, the strongest hybridization signals were detected in the segments containing stages VII VIII and IX XII of the seminiferous epithelium. Ionizing radiation (1-12 Gy) induced the expression of p21WAF1 in a dose-dependent manner and the lowest dose that showed a clear increase in mRNA levels was 3 Gy. The p21WAF1 mRNA levels peaked after 3-4 hours, but remained high compared with the control levels during the 24-h follow-up. No change in the in situ hybridization pattern was seen when comparing unirradiated and irradiated tissue. Thus, it appears that X-irradiation induces p21WAF1 in the pachytene spermatocytes. Since p21WAF1 mRNA was found in pachytene spermatocytes and in round spermatids in normal testis, the protein may take part in the regulation of meiosis and in the 'terminal' differentiation of the male germ cells.

  16. Cyclin-dependent kinase inhibitor, p21Waf1, regulates vascular smooth muscle cell hypertrophy.

    PubMed

    Okamoto, Kenichi; Kato, Seiya; Arima, Nobuyuki; Fujii, Teruhiko; Morimatsu, Minoru; Imaizumi, Tsutomu

    2004-04-01

    In the process of vascular diseases, smooth muscle cells (SMC) undergo not only hyperplasia but also hypertrophy, resulting in vascular remodeling. A cyclin-dependent kinase inhibitor (CDKI), p21Waf1, has been shown to play an important role in SMC hyperplasia. Here we investigated a potential role of p21Waf1 in SMC hypertrophy. An exposure of cultured rat SMC to serum drove the cell cycle progression with up-regulation of various cell cycle markers and increased activities of cyclin-dependent kinases, but did not cause SMC hypertrophy. In contrast, incubation of SMC for 48 h with angiotensin II (AII, 100 nmol/l) resulted in a significant increase in the cell size measured by flowcytometric forward-angle light scatter assay, in association with an increase in the ratio of [3H]leucine/[3H]thymidine uptake, indicating SMC hypertrophy. At 48 h, p21Waf1 expression was up-regulated in SMC exposed to AII but not in those exposed to serum. These results suggest that p21Waf1 may be involved in hypertrophy. To further investigate this issue, two manipulations of the p21Waf1 gene were performed. Adenovirus-mediated over-expression of p21Waf1 not only reduced S-phasic cells but also caused hypertrophy, despite the exposure to serum. Antisense oligodeoxynucleotide for p21Waf1 inhibited the hypertrophy of SMC exposed to AII. Our data suggest that p21Waf1 may play a role in SMC hypertrophy as well.

  17. Accommodating Picky Palates

    ERIC Educational Resources Information Center

    Lum, Lydia

    2007-01-01

    Healthy gourmet offerings are fast becoming the norm at college dining halls around the country. At a time when the children of Baby Boomers are hitting higher education in record numbers, college officials have scrambled to accommodate their picky palates and their insistence for healthier meals than were served to past generations. At the same…

  18. Studies in Binocular Accommodation.

    NASA Astrophysics Data System (ADS)

    Winn, Barry

    1987-09-01

    Available from UMI in association with The British Library. Requires signed TDF. A study of the binocular accommodation response is presented for normal and amblyopic observers to selected stimulus conditions using a binocular infra-red optometer and a commercially-available autorefractor. The work reviews the neural control of the near triad and discusses the historical development of models of mutual interaction between accommodation and convergence, presenting experimental evidence to support or refute each proposition. The basic characteristics of the accommodation response are reviewed along with the influencing factors. A central feature of this work is the evaluation of the correlation present between the eyes for both step -wise changes in target vergence and steady-state viewing. Reaction times for visually normal subjects were found to be similar to those found by previous workers and were independent of both size and direction of the step change. Response times for a mean step size of approximately 2.5D exhibited a marked degree of intersubject variability, particularly for the decreasing response and were step -size dependent. Eye dominancy was not found to be a significant factor in the overall response time. The binocular accommodation responses were found to have a high level of correlation to step-wise changes in target vergence. Assessment of the microfluctuations was necessary. A high degree of correlation between amblyopic eyes and their fellow normal eyes is reported for both reaction and response times. Steady-state viewing shows the microfluctuations to have a high level of coherence. As target luminance decreased, rms values and low frequency drifts increased. Amblyopic eyes show an increase in the magnitude of the low frequency components of the microfluctuations for moderate to high stimulus vergences. The presence of different behaviour to that observed in normals supports a role for the microfluctuations. The response of amblyopic eyes to

  19. [The mechanisms of p21WAF1/Cip-1 expression in MOLT-4 cell line induced by TSA].

    PubMed

    Song, Yi; Liu, Mei-Ju; Zhao, Guo-Wei; Qian, Jun-Jie; Dong, Yan; Liu, Hua; Sun, Guo-Jing; Mei, Zhu-Zhong; Liu, Bin; Tian, Bao-Lei; Sun, Zhi-Xian

    2005-04-01

    To investigate the function and molecular mechanism of p21(WAF1/Cip-1) expression in MOLT-4 cells induced by HDAC inhibitor TSA, the expression pattern of p21(WAF1/Cip-1) and the distribution of cell cycle in TSA treated cells were analyzed. The results showed that TSA could effectively induce G(2)/M arrest and apoptosis of MOLT-4 cells. Kinetic experiments demonstrated that p21(WAF1/Cip-1) were upregulated quickly before cell arrested in G(2)/M and began decreasing at the early stage of apoptosis. Meanwhile, the proteasome inhibitor MG-132 could inhibit the decrease of p21(WAF1/Cip-1) at the early stage of apoptosis, which showed that proteasome pathway involved in p21(WAF1/Cip-1) degradation during the TSA induced G(2)/M arrest and apoptosis responses. This study also identified that the protein level of p21(WAF1/Cip-1) was highly associated with the cell cycle change induced by TSA. Compared to cells treated by TSA only, exposure MOLT-4 cells to TSA meanwhile treatment with MG-132 increased the protein level of p21(WAF1/Cip-1) and increased the numbers of cell in G(2)/M-phase, whereas the cell apoptosis were delayed. It is concluded that p21(WAF1/Cip-1) plays a significant role in G(2)/M arrest and apoptosis signaling induced by TSA in MOLT-4 cells.

  20. Visual accommodation trainer-tester

    NASA Technical Reports Server (NTRS)

    Randle, R. J., Jr. (Inventor)

    1983-01-01

    An apparatus for training of the human visual accommodation system is presented, specifically, useful for training a person to volitionally control his focus to his far point (normaly infinity) from a position of myopia due to functional causes. The functional causes could be due, for example, to a behavioral accommodative spasm or the effects of an empty field. The device may also be used to measure accommodation, the accommodation resting position and the near and far points of vision.

  1. [Spasm of accommodation].

    PubMed

    Lindberg, Laura

    2014-01-01

    Spasm of accommodation refers to prolonged contraction of the ciliary muscle, most commonly causing pseudomyopia to varying degrees in both eyes by keeping the lens in a state of short sightedness. It may also be manifested as inability to allow the adaptation spasticity prevailing in the ciliary muscle relax without measurable myopia. As a rule, this is a functional ailment triggered by prolonged near work and stress. The most common symptoms include blurring of distance vision, varying visual acuity as well as pains in the orbital region and the head, progressing into a chronic state. Cycloplegic eye drops are used as the treatment.

  2. Inducible expression of p21WAF-1/CIP-1/SDI-1 from a promoter conversion retroviral vector.

    PubMed

    Mrochen, S; Klein, D; Nikol, S; Smith, J R; Salmons, B; Günzburg, W H

    1997-01-01

    Constitutive, high-level expression of the potentially therapeutic WAF-1/CIP-1/SDI-1 gene is incompatible with cell growth. A promoter conversion retroviral vector carrying the WAF-1/CIP-1/SDI-1 gene under the transcriptional control of the glucocorticoid inducible promoter of mouse mammary tumor virus was used to infect human bladder carcinoma or feline kidney cells. Reduced cell growth due to a greater proportion of cells being in the G0/G1 phase of the cell cycle was observed when WAF-1/CIP-1/SDI-1 expression was activated by addition of glucocorticoid hormone. This system demonstrates the potential long-term therapeutic use of WAF-1/CIP-1/SDI-1 delivered by retroviral vectors for inhibiting the growth of rapidly proliferating cells. Moreover, the conditional expression of genes such as WAF-1/CIP-1/SDI-1 from such retroviral vectors may facilitate analysis of their function.

  3. Protective role of p21(Waf1/Cip1) against prostaglandin A2-mediated apoptosis of human colorectal carcinoma cells.

    PubMed Central

    Gorospe, M; Wang, X; Guyton, K Z; Holbrook, N J

    1996-01-01

    Prostaglandin A2 (PGA2) suppresses tumor growth in vivo, is potently antiproliferative in vitro, and is a model drug for the study of the mammalian stress response. Our previous studies using breast carcinoma MCF-7 cells suggested that p21(Waf1/Cip1) induction enabled cells to survive PGA2 exposure. Indeed, the marked sensitivity of human colorectal carcinoma RKO cells to the cytotoxicity of PGA2 is known to be associated with a lack of a PGA2-mediated increase in p21(Waf1/Cip1) expression, inhibition of cyclin-dependent kinase activity, and growth arrest. To determine if cell death following exposure to PGA2 could be prevented by forcing the expression of p21(Waf1/Cip1) in RKO cells, we utilized an adenoviral vector-based expression system. We demonstrate that ectopic expression of p21(Waf1/Cip1) largely rescued RKO cells from PGA2-induced apoptotic cell death, directly implicating p21(Waf1/Cip1) as a determinant of the cellular outcome (survival versus death) following exposure to PGA2. To discern whether p21(Waf1/Cip1)-mediated protection operates through the implementation of cellular growth arrest, other growth-inhibitory treatments were studied for the ability to attenuate PGA2-induced cell death. Neither serum depletion nor suramin (a growth factor receptor antagonist) protected RKO cells against PGA2 cytotoxicity, and neither induced p21(Waf1/Cip1) expression. Mimosine, however, enhanced p21(Waf1/Cip1) expression, completely inhibited RKO cell proliferation, and exerted marked protection against a subsequent PGA2 challenge. Taken together, our results directly demonstrate a protective role for p21(Waf1/Cip1) during PGA2 cellular stress and provide strong evidence that the implementation of cellular growth arrest contributes to this protective influence. PMID:8943319

  4. Retinal Image Quality During Accommodation

    PubMed Central

    López-Gil, N.; Martin, J.; Liu, T.; Bradley, A.; Díaz-Muñoz, D.; Thibos, L.

    2013-01-01

    Purpose We asked if retinal image quality is maximum during accommodation, or sub-optimal due to accommodative error, when subjects perform an acuity task. Methods Subjects viewed a monochromatic (552nm), high-contrast letter target placed at various viewing distances. Wavefront aberrations of the accommodating eye were measured near the endpoint of an acuity staircase paradigm. Refractive state, defined as the optimum target vergence for maximising retinal image quality, was computed by through-focus wavefront analysis to find the power of the virtual correcting lens that maximizes visual Strehl ratio. Results Despite changes in ocular aberrations and pupil size during binocular viewing, retinal image quality and visual acuity typically remain high for all target vergences. When accommodative errors lead to sub-optimal retinal image quality, acuity and measured image quality both decline. However, the effect of accommodation errors of on visual acuity are mitigated by pupillary constriction associated with accommodation and binocular convergence and also to binocular summation of dissimilar retinal image blur. Under monocular viewing conditions some subjects displayed significant accommodative lag that reduced visual performance, an effect that was exacerbated by pharmacological dilation of the pupil. Conclusions Spurious measurement of accommodative error can be avoided when the image quality metric used to determine refractive state is compatible with the focusing criteria used by the visual system to control accommodation. Real focusing errors of the accommodating eye do not necessarily produce a reliably measurable loss of image quality or clinically significant loss of visual performance, probably because of increased depth-of-focus due to pupil constriction. When retinal image quality is close to maximum achievable (given the eye’s higher-order aberrations), acuity is also near maximum. A combination of accommodative lag, reduced image quality, and reduced

  5. The Accommodation Operation. Accommodation Management Module. Operational Management Programme.

    ERIC Educational Resources Information Center

    Chapman, Janet

    This module on accommodation operation is intended to help supervisors or managers achieve a balance in the day-to-day running of the premises and plan for a smooth and successful future. Much of the material is concerned with the housekeeping aspects of accommodation management. The material is presented in a self-instructional format in seven…

  6. Apoptotic effect of ethyl-4-isothiocyanatobutanoate is associated with DNA damage, proteasomal activity and induction of p53 and p21cip1/waf1.

    PubMed

    Bodo, Juraj; Jakubikova, Jana; Chalupa, Ivan; Bartosova, Zdena; Horakova, Katarina; Floch, Lubomir; Sedlak, Jan

    2006-08-01

    The effect of synthetic isothiocyanate ethyl-4-isothiocyanatobutanoate (E-4IB) on survival of mismatch repair-proficient TK6 and -deficient MT1 cell lines as well as the influence of proteasomal inhibitor MG132, caspase inhibitor Z-VAD-fmk, and ATM inhibitor caffeine on E-4IB modulation of cell cycle and apoptosis was evaluated. Flow cytometric analyses of DNA double strand breaks (gamma-H2AX), mitotic fraction (phospho-histone H3), cell cycle modulation, apoptosis induction (sub-G(0) fraction and fluorescein diacetate staining), and dissipation of transmembrane mitochondrial potential (JC-1 staining) were performed. Western blotting was used for the evaluation of ERK activation, expression of p53, p21(cip1/waf1) and GADD45alpha proteins, as well as PARP fragmentation. Analysis of mitotic nuclei was performed for chromosomal aberrations assessment. MT1 cells were more resistant to E-4IB treatment then TK6 cells (IC(50) 8 muM vs. 4 muM). In both cell lines E-4IB treatment induced phosphorylation of H2AX, increase of p53 protein level, phospho-histone H3 staining, and G(2)/M arrest. The sub-G(0) fragmentation was accompanied by PARP degradation, decreased mitochondrial transmembrane potential, and diminished p21(cip1/waf1) protein expression in TK6 cells. Caspase inhibitor Z-VAD-fmk decreased E-4IB induced sub-G(0) fragmentation and extent of apoptosis in TK6 cells, while proteasome inhibitor MG132 increased number of apoptotic cells in both cell lines tested. A number of aberrant metaphases and clastogenic effect of high E-4IB concentration was observed. The synthetic isothiocyanate E-4IB induced DNA strand breaks, increased mitotic fraction and apoptosis potentiated by MG132 inhibitor in both mismatch repair-proficient and -deficient cell lines.

  7. Anthropometric accommodation in USAF cockpits

    NASA Technical Reports Server (NTRS)

    Zehner, Gregory F.

    1994-01-01

    Over the past three years, a new set of methodologies has been developed to specify and evaluate anthropometric accommodation in USAF crewstation designs. These techniques are used to improve the ability of the pilot to reach controls, to safely escape the aircraft, to achieve adequate mobility and comfort, and to assure full access to the visual field both inside and outside the aircraft. This paper summarized commonly encountered aircraft accommodation problems, explains the failure of the traditional 'percentile man' design concept to resolve these difficulties, and suggests an alternative approach for improving cockpit design to better accommodate today's more heterogeneous flying population.

  8. The immunohistochemical expression of p21WAF1/Cip1 and Proliferating cell nuclear antigen in laryngeal squamous cell carcinomas.

    PubMed

    Li, X; Izumaru, S; Sakamoto, K; Miyajima, Y; Nakashima, T

    2006-12-01

    The purpose of this study was to assess the significance of the expression of p21WAF1/Cip1 and proliferating cell nuclear antigen (PCNA) in human laryngeal squamous cell carcinomas. Forty-eight patients (25 supraglottic, 23 glottic) who had undergone operations between 1998 and 2003 were included in this study. The Envision immunohistochemistry method was utilized to stain these tissue specimens. The results showed that the immunostaining of p21WAF1/Cip1 do not show any correlation with differentiation, N stage, metastasis, survival, recurrence or the laryngeal site of involvement. A significant inverse correlation was detected between p21WAF1/Cip1 and the T stage. In contrast, the percentage of PCNA labelled cells showed a significant correlation with the T stage, but not with other clinicopathological parameters. There were differences in the expression of p21WAF1/Cip1 and PCNA between the supraglottic and the glottic carcinomas. In conclusion, our findings suggest that p21WAF1/Cip1 and PCNA may play an important role in the progression of carcinoma of the supraglottic larynx.

  9. Statins inhibit pulmonary artery smooth muscle cell proliferation by upregulation of HO-1 and p21WAF1.

    PubMed

    Li, Manxiang; Liu, Yuan; Shi, Hongyang; Zhang, Yonghong; Wang, Guizuo; Xu, Jing; Lu, Jiamei; Zhang, Dexin; Xie, Xinming; Han, Dong; Wu, Yuanyuan; Li, Shaojun

    2012-10-01

    Simvastatin is a 3-hydroxy-3-methylglutaryl-CoA reductase inhibitor, which has been shown to ameliorate the development of pulmonary hypertension in animal model by suppression of pulmonary artery smooth muscle cells (PASMCs) proliferation, yet its underlying molecular mechanisms are not completely understood. In this study, we show that simvastatin dose-dependently inhibited serotonin-stimulated PASMCs proliferation. This was accompanied with the parallel induction of heme oxyganase-1 (HO-1) and upregulation of p21(WAF1). More importantly, we found that Tin-protoporphyrin (SnPP), a selective inhibitor of HO-1, could block the effect of simvastatin on inhibition of cell proliferation in response to serotonin and abolish simvastatin-induced p21(WAF1) expression. The inhibitive effect of simvastatin on cell proliferation was also significantly suppressed by silencing p21(WAF1) with siRNA transfection. The extent of effect of SnPP on inhibition of cell proliferation was similar to that of lack of p21(WAF1) by siRNA transfection. Taken together, our study suggests that simvastatin inhibits PASMCs proliferation by sequential upregulation of HO-1 and p21(WAF1) to benefit pulmonary hypertension.

  10. Expression of Smad4, TGF-βRII, and p21waf1 in esophageal squamous cell carcinoma tissue

    PubMed Central

    CHENG, HUI; CHEN, CHENG; LIU, LU; ZHAN, NA; LI, BENHUI

    2015-01-01

    Esophageal squamous cell carcinoma (SCC) possesses one of the worst prognoses out of the digestive carcinomas. Several studies have suggested that transforming growth factor β receptor type II (TGF-βRII), Smad family member 4 (Smad4) and p21 wild-type p53-activated factor 1 (p21waf1) are associated with esophageal SCC. The aim of the present study was to evaluate the effect of Smad4, TGF-βRII and p21waf1 in esophageal squamous cancer tissue and the pathological significance of the effect. An immunohistochemical method was used to evaluate the expression levels of Smad4, TGF-βRII and p21waf1 in specimens of esophageal SCC lesions obtained from 80 patients. It was found that the expression of Smad4, TGF-βRII and p21waf1 in histologically-classified grade I esophageal SCC, without invasion or lymph node metastasis, was markedly higher compared with grade III esophageal SCC that had invaded into the deep muscular or serous layer and metastasized to the lymph nodes (P<0.05). Analysis of the expression level of Smad4, TGF-βRII and p21waf1, as well as the clinical and pathological characteristics of esophageal SCC, revealed that the three proteins may be associated with the carcinogenesis, biological behavior and prognosis of esophageal SCC, parallel to the pathological stage and cell grade. PMID:26137158

  11. p21WAF1/Cip1 expression is associated with cell differentiation but not with p53 mutations in squamous cell carcinomas of the larynx.

    PubMed

    Nadal, A; Jares, P; Cazorla, M; Fernández, P L; Sanjuan, X; Hernandez, L; Pinyol, M; Aldea, M; Mallofré, C; Muntané, J; Traserra, J; Campo, E; Cardesa, A

    1997-10-01

    p21WAF1/Cip1 is a recently identified gene involved in cell cycle regulation through cyclin-CDK-complex inhibition. The expression of this gene in several cell lines seems to be induced by wild-type, but not mutant, p53. p21WAF1/Cip1 expression has been studied at both mRNA and protein levels in a series of 49 normal mucosae and squamous cell carcinomas of the larynx. A significant association was found between mRNA and protein expression in tumours (P < 0.0001). p21WAF1/Cip1 expression was strongly associated with squamous cell differentiation of carcinomas, because six of seven (86 per cent) undifferentiated carcinomas (grade 4) showed very low levels of p21WAF1/Cip1 expression, whereas 41 out of 42 (98 per cent) carcinomas with squamous cell differentiation (grades 1-3) had normal or high levels of p21WAF1/Cip1 expression (P < 0.0001). In addition, p21WAF1/Cip1 expression was topologically related to the squamous differentiation of tumour cells with a distribution similar to that seen in normal squamous epithelium. No correlation was found between p21WAF1/Cip1 expression and the global S-phase of the carcinomas. p53 mutations (exons 5-9) were found in ten carcinomas with p21WAF1/Cip1 expression, but no p53 mutations were detected in three p21WAF1/Cip1-negative tumours. In conclusion, p21WAF1/Cip1 expression is frequently upregulated in squamous cell carcinomas of the larynx and is associated with tumour cell differentiation. p21WAF1/Cip1 expression in these tumours is independent of p53 gene mutations.

  12. p21{sup WAF1/Cip1/Sdi1} knockout mice respond to doxorubicin with reduced cardiotoxicity

    SciTech Connect

    Terrand, Jerome; Xu, Beibei; Morrissy, Steve; Dinh, Thai Nho; Williams, Stuart; Chen, Qin M.

    2011-11-15

    Doxorubicin (Dox) is an antineoplastic agent that can cause cardiomyopathy in humans and experimental animals. As an inducer of reactive oxygen species and a DNA damaging agent, Dox causes elevated expression of p21{sup WAF1/Cip1/Sdi1} (p21) gene. Elevated levels of p21 mRNA and p21 protein have been detected in the myocardium of mice following Dox treatment. With chronic treatment of Dox, wild type (WT) animals develop cardiomyopathy evidenced by elongated nuclei, mitochondrial swelling, myofilamental disarray, reduced cardiac output, reduced ejection fraction, reduced left ventricular contractility, and elevated expression of ANF gene. In contrast, p21 knockout (p21KO) mice did not show significant changes in the same parameters in response to Dox treatment. In an effort to understand the mechanism of the resistance against Dox induced cardiomyopathy, we measured levels of antioxidant enzymes and found that p21KO mice did not contain elevated basal or inducible levels of glutathione peroxidase and catalase. Measurements of 6 circulating cytokines indicated elevation of IL-6, IL-12, IFN{gamma} and TNF{alpha} in Dox treated WT mice but not p21KO mice. Dox induced elevation of IL-6 mRNA was detected in the myocardium of WT mice but not p21KO mice. While the mechanism of the resistance against Dox induced cardiomyopathy remains unclear, lack of inflammatory response may contribute to the observed cardiac protection in p21KO mice. -- Highlights: Black-Right-Pointing-Pointer Doxorubicin induces p21 elevation in the myocardium. Black-Right-Pointing-Pointer Doxorubicin causes dilated cardiomyopathy in wild type mice. Black-Right-Pointing-Pointer p21 Knockout mice are resistant against doxorubicin induced cardiomyopathy. Black-Right-Pointing-Pointer Lack of inflammatory response correlates with the resistance in p21 knockout mice.

  13. 43 CFR 17.211 - Reasonable accommodation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental... that the accommodation would impose an undue hardship on the operation of its program or activity. (b... accommodation would impose an undue hardship on the operation of a recipient's program or activity, factors...

  14. 24 CFR 100.204 - Reasonable accommodations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Reasonable accommodations. 100.204... Handicap § 100.204 Reasonable accommodations. (a) It shall be unlawful for any person to refuse to make reasonable accommodations in rules, policies, practices, or services, when such accommodations may...

  15. 24 CFR 100.204 - Reasonable accommodations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Reasonable accommodations. 100.204... Handicap § 100.204 Reasonable accommodations. (a) It shall be unlawful for any person to refuse to make reasonable accommodations in rules, policies, practices, or services, when such accommodations may...

  16. Accommodations for Multiple Choice Tests

    ERIC Educational Resources Information Center

    Trammell, Jack

    2011-01-01

    Students with learning or learning-related disabilities frequently struggle with multiple choice assessments due to difficulty discriminating between items, filtering out distracters, and framing a mental best answer. This Practice Brief suggests accommodations and strategies that disability service providers can utilize in conjunction with…

  17. Reasonable Accommodation in Training Safety.

    ERIC Educational Resources Information Center

    Sandoz, Jeff

    A pictograph and icon-driven training program has been specifically designed for educators who are responsible for teaching the developmentally disabled regarding the safe use of hazardous chemicals. In alignment with the Americans with Disabilities Act, it offers "reasonable accommodation" by those who educate and train this special…

  18. Patterns of p21waf1/cip1 expression in non-papillomatous nasal mucosa, endophytic sinonasal papillomas, and associated carcinomas

    PubMed Central

    Schwerer, M; Sailer, A; Kraft, K; Baczako, K; Maier, H

    2001-01-01

    Aims—To clarify p21waf1/cip1 expression in sinonasal lesions. Methods—Archived surgical specimens from 38 patients were investigated by means of immunohistochemistry. p21waf1/cip1 staining was evaluated in the different layers of the epithelium. In addition, human papillomavirus (HPV) infection and p53 protein overexpression were assessed and correlated with p21waf1/cip1 expression. Results—p21waf1/cip1 staining was negative in non-papillomatous nasal mucosa. HPV infection and p53 protein overexpression were not seen. Sixteen of 20 inverted papillomas showed p21waf1/cip1 expression. HPV infection was found in 16 cases and p53 protein overexpression was present in 13 specimens. Expression of p21waf1/cip1 was restricted to surface cells in five cases, but involved basal/parabasal cells in 11 specimens. Immunoreactivity for p21waf1/cip1 in basal/parabasal cells colocalised with p53 protein overexpression. Enhanced expression rates for p21waf1/cip1 were seen in transitional and squamous epithelium compared with columnar epithelium. p21waf1/cip1 expression involved only surface cells in cylindrical cell papillomas. HPV infection and p53 protein overexpression were detected in all specimens. One of five squamous cell carcinomas showed p21waf1/cip1 expression. HPV infection was seen in two cases, and all carcinomas showed p53 protein overexpression. Conclusions—Expression of p21waf1/cip1 is associated with terminal differentiation in surface cells in inverted papillomas and cylindrical cell papillomas, but not in non-papillomatous nasal mucosa. Overexpression of p53 protein colocalises with p21waf1/cip1 expression in basal/parabasal cells in inverted papillomas but not in cylindrical cell papillomas. Expression of p21waf1/cip1 in squamous cell carcinomas involves a subset of tumours with p53 protein overexpression. Key Words: p21waf1/cip1 • nasal mucosa • sinonasal papillomas • squamous cell carcinomas PMID:11684723

  19. STS payload data collection and accommodations analysis study. Volume 3: Accommodations analysis

    NASA Technical Reports Server (NTRS)

    1978-01-01

    Payload requirements were compared to launch site accommodations and flight accommodations for a number of Spacelab payloads. Experiment computer operating system accommodations were also considered. A summary of accommodations in terms of resources available for payload discretionary use and recommendations for Spacelab/STS accommodation improvements are presented.

  20. International Space Station payload accommodations

    NASA Astrophysics Data System (ADS)

    Hartman, Daniel W.

    1999-01-01

    The International Space Station (ISS) is a low Earth orbiting facility for conducting research in life science, microgravity, Earth observations, and Engineering Research and Technology. Assembled on-orbit at a nominal altitude of 220 nautical miles, it will provide a shirt-sleeve environment for conducting research in six laboratories: the US Laboratory (US Lab), the Japanese Experiment Module (JEM), the European Columbus Orbiting Facility (COF), the Centrifuge Accommodations Module (CAM), and the Russian Research Modules. Supplies will be replenished using the Multi-Purpose Pressurized Logistics Module (MPLM), a conditioned pressurized transport carrier which will also return passive and perishable payload cargo to earth. External Earth observations can be performed by utilizing the payload attachment points on the truss, the Russian Science Power Platform, the JEM Exposed Facility (EF), and the COF backporch. The pressurized and external locations are equipped with a variety of electrical, avionics, fluids, and gas interfaces to support the experiments. ISS solar arrays, thermal radiators, communication system, propulsion, environmental control, and robotic devices provide the infrastructure to support sustained research. This paper, which reflects the design maturity of payload accommodations at the time of its submittal (10/20/98), is primarily based on the assembly complete configuration of the station. As the design matures, ISS Payload Accommodations will be updated to reflect qualification tests of components and associated analyses of the integrated performance.

  1. Independent and reciprocal accommodation in anisometropic amblyopia

    PubMed Central

    Horwood, Anna M.; Riddell, Patricia M

    2015-01-01

    Accommodation is considered to be a symmetrical response, and to be driven by the least ametropic and nonamblyopic eye in anisometropia. We report a case of a 4-year-old child with anisometropic amblyopia who accommodates asymmetrically, reliably demonstrating normal accommodation in the nonamblyopic eye and anti-accommodation of the amblyopic eye to near targets. The abnormal accommodation of the amblyopic eye remained largely unchanged over seven subsequent testing sessions undertaken over the course of therapy. We suggest that a congenital dysinnervation syndrome may result in relaxation of accommodation in relation to near cues and might be a hitherto-unconsidered additional etiologic factor in anisometropic amblyopia. PMID:20863728

  2. p21{sup WAF1} modulates NF-{kappa}B signaling and induces anti-apoptotic protein Bcl-2 in Tax-expressing rat fibroblast

    SciTech Connect

    Akita, Kazumasa; Kawata, Sanae; Shimotohno, Kunitada . E-mail: kshimoto@virus.kyoto-u.ac.jp

    2005-02-05

    Of the cell cycle-associated genes regulated by human T-cell leukemia virus type-1 (HTLV-1) Tax, cyclin-dependent kinase (CDK) inhibitor p21{sup WAF1} is upregulated in HTLV-1-infected cells. Previously, we reported that p21{sup WAF1} stimulated Tax-dependent NF-{kappa}B activation which influences a variety of cellular processes, including proliferation, differentiation, and apoptosis. In HTLV-1-infected cells, Tax is primarily involved in the constitutive activation of NF-{kappa}B signaling. Here, we demonstrate that p21{sup WAF1} affects Tax-dependent NF-{kappa}B signaling by inducing p100/52, an NF-{kappa}B-related protein. W4, a Tax-transformed rat fibroblast cell line, exhibits the constitutive activation of NF-{kappa}B signaling, potentially mediated by overexpression of RelB. Ectopic expression of p21{sup WAF1} in W4 cells, which lack endogenous expression due to methylation of the p21{sup WAF1} promoter, induces the expression of p100/52. Bcl-2 expression was also upregulated by ectopic p21{sup WAF1} in this cell line, suggesting that p21{sup WAF1} plays an important role in the regulation of apoptosis by modulating NF-{kappa}B signaling in Tax-expressing rat fibroblasts. We also address the expression of NF-{kappa}B-related proteins in HTLV-1-infected cells.

  3. p53 missense but not truncation mutations are associated with low levels of p21CIP1/WAF1 mRNA expression in primary human sarcomas

    PubMed Central

    Mousses, S; Gokgoz, N; Wunder, J S; Ozcelik, H; Bull, S; Bell, R S; Andrulis, I L

    2001-01-01

    Many growth-suppressing signals converge to control the levels of the CDK inhibitor p21CIP1/WAF1. Some human cancers exhibit low levels of expression of p21CIP1/WAF1and mutations in p53 have been implicated in this down-regulation. To evaluate whether the presence of p53 mutations was related to the in vivo expression of p21CIP1/WAF1 mRNA in sarcomas we measured the p21CIP1/WAF1 mRNA levels for a group of 71 primary bone and soft tissue tumours with known p53 status. As expected, most tumours with p53 mutations expressed low levels of p21CIP1/WAF1 mRNA. However, we identified a group of tumours with p53 gene mutations that exhibited normal or higher levels of p21CIP1/WAF1 mRNA. The p53 mutations in the latter group were not the common missense mutations in exons 4–9, but were predominantly nonsense mutations predicted to result in truncation of the p53 protein. The results of this study suggest that different types of p53 mutations can have different effects on the expression of downstream genes such as p21CIP1/WAF1 in human sarcomas. © 2001 Cancer Research Campaign http://www.bjcancer.com PMID:11401317

  4. p21waf1/cip1 deficiency does not perturb the intestinal crypt stem cell population after massive small bowel resection

    PubMed Central

    Longshore, Shannon W.; Nair, Rajalakshmi; Perrone, Erin E.; Erwin, Christopher R.; Guo, Jun; Warner, Brad W.

    2009-01-01

    Background After small bowel resection (SBR), adaptation is initiated in intestinal crypts where stem cells reside. Prior studies revealed SBR induced enterocyte proliferation requires the expression of p21waf1/cip1. Since deficient expression of p21waf1/cip1 has been shown to result in reduced numbers of hematopoietic stem cells, we sought to test the hypothesis that p21waf1/cip1 deficiency similarly perturbs the intestinal stem cell population after SBR. Methods Control (n=21; C57Bl/6) and p21waf1/cip1-null mice (n=30) underwent 50% proximal SBR or sham operation. After 3 days, the ileum was harvested and the crypt stem cell population evaluated by counting crypt base columnar (CBC) cells on histological sections, determining the expression of Musashi-1 and Lgr5, and profiling the transcriptional expression of 84 known stem cell genes. Results There were no significant differences in CBC cells, expression of Musashi-1 or Lgr5, or in stem cell gene expression after SBR in control mice. Further, there were no differences in these markers between controls and p21waf1/cip1-null mice. Conclusion In contrast with bone marrow stem cells, the stem cell population of the gut is unaffected by deficient expression of p21waf1/cip1. Additional mechanisms for the role of p21waf1/cip1 in small bowel proliferation and adaptation following massive SBR must be considered. PMID:19524718

  5. Mitomycin C and decarbamoyl mitomycin C induce p53-independent p21WAF1/CIP1 activation

    PubMed Central

    Cheng, Shu-Yuan; Seo, Jiwon; Huang, Bik Tzu; Napolitano, Tanya; Champeil, Elise

    2016-01-01

    Mitomycin C (MC), a commonly used anticancer drug, induces DNA damage via DNA alkylation. Decarbamoyl mitomycin C (DMC), another mitomycin lacking the carbamate at C10, generates similar lesions as MC. Interstrand cross-links (ICLs) are believed to be the lesions primarily responsible for the cytotoxicity of MC and DMC. The major ICL generated by MC (α-ICL) has a trans stereochemistry at the guanine-drug linkage whereas the major ICL from DMC (β-ICL) has the opposite, cis, stereochemistry. In addition, DMC can provoke strong p53-independent cell death. Our hypothesis is that the stereochemistry of the major unique β-ICL generated by DMC is responsible for this p53-independent cell death signaling. p53 gene is inactively mutated in more than half of human cancers. p21WAF1/CIP1 known as a major effector of p53 is involved in p53-dependent and -independent control of cell proliferation and death. This study revealed the role of p21WAF1/CIP1 on MC and DMC triggered cell damage. MCF-7 (p53-proficient) and K562 (p53-deficient) cells were used. Cell cycle distributions were shifted to the G1/S phase in MCF-7 treated with MC and DMC, but were shifted to the S phase in K562. p21WAF1/CIP1 activation was observed in both cells treated with MC and DMC, and DMC triggered more significant activation. Knocking down p53 in MCF-7 did not attenuate MC and DMC induced p21WAF1/CIP1 activation. The α-ICL itself was enough to cause p21WAF1/CIP1 activation. PMID:27666201

  6. Accommodative spasm in siblings: A unique finding

    PubMed Central

    Rutstein, Robert P

    2010-01-01

    Accommodative spasm is a rare condition occurring in children, adolescents, and young adults. A familial tendency for this binocular vision disorder has not been reported. I describe accommodative spasm occurring in a brother and sister. Both children presented on the same day with complaints of headaches and blurred vision. Treatment included cycloplegia drops and bifocals. Siblings of patients having accommodative spasm should receive a detailed eye exam with emphasis on recognition of accommodative spasm. PMID:20534925

  7. Telemedicine for AIDS patients accommodations.

    PubMed

    Kulik, J F; de la Tribonnière, X; Bricon-Souf, N; Beuscart, R J; Mouton, Y

    1997-01-01

    People suffering from AIDS are subject to frequent hospitalisations. In some cases, they cannot go back home after hospitalisations, due to severe illness, family or sociologic problems. This is the reason why some therapeutic flats are at their disposal to make easier their medical follow-up after the hospital's discharge. In these Therapy Accommodation, they are treated by trained GP who often suffer from lack of information and lack of expertise in difficult cases. For this purpose we included these flats in the regional Telemedicine AIDS network to give these physicians free access to the computerised multimedia medical record of their patients and to provide them with synchronous co-operation facilities.

  8. Modal Identification Experiment accommodations review

    NASA Technical Reports Server (NTRS)

    Klich, Phillip J.; Stillwagen, Frederic H.; Mutton, Philip

    1994-01-01

    The Modal Identification Experiment (MIE) will monitor the structure of the Space Station Freedom (SSF), and measure its response to a sequence of induced disturbances. The MIE will determine the frequency, damping, and shape of the important modes during the SSF assembly sequence including the Permanently Manned Configuration. This paper describes the accommodations for the proposed instrumentation, the data processing hardware, and the communications data rates. An overview of the MIE operational modes for measuring SSF acceleration forces with accelerometers is presented. The SSF instrumentation channel allocations and the Data Management System (DMS) services required for MIE are also discussed.

  9. Procedural Guide to Accommodating Adults with Disabilities.

    ERIC Educational Resources Information Center

    Mellard, Daryl

    This guide is designed to provide a framework and sequence for working jointly with adult learners to confirm their disability, assess their functional needs, select appropriate accommodations, provide instruction for using accommodations, and monitor accommodation effectiveness. In addition to procedural information, the guide contains instructor…

  10. 22 CFR 142.12 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Employment Practices § 142.12 Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental limitations... the accommodation would impose an undue hardship on the operation of the program or activity....

  11. 22 CFR 217.12 - Reasonable accommodation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Employment Practices § 217.12 Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental limitations... the accommodation would impose an undue hardship on the operation of its program or activity....

  12. 7 CFR 15b.13 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Employment Practices § 15b.13 Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental limitations of an... accommodation would impose an undue hardship on the operation of its program or activity. (b)...

  13. 22 CFR 142.12 - Reasonable accommodation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Employment Practices § 142.12 Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental limitations... the accommodation would impose an undue hardship on the operation of the program or activity....

  14. 22 CFR 217.12 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Employment Practices § 217.12 Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental limitations... the accommodation would impose an undue hardship on the operation of its program or activity....

  15. 14 CFR 1251.201 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Reasonable accommodation. 1251.201 Section... OF HANDICAP Employment Practices § 1251.201 Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental limitations of an otherwise qualified...

  16. 45 CFR 1170.22 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Reasonable accommodation. 1170.22 Section 1170.22... ASSISTED PROGRAMS OR ACTIVITIES Employment Practices § 1170.22 Reasonable accommodation. (a) A recipient shall make reasonable accommodation to the known physical or mental limitations of an...

  17. 45 CFR 1151.32 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... operation of its program or activity. (b) Reasonable accommodation may include: (1) Making facilities used... 45 Public Welfare 3 2010-10-01 2010-10-01 false Reasonable accommodation. 1151.32 Section 1151.32... Prohibited Employment § 1151.32 Reasonable accommodation. (a) A recipient shall make reasonable...

  18. 46 CFR 169.317 - Accommodations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... separating accommodations from machinery spaces, paint lockers, storerooms, washrooms, and toilet facilities... 46 Shipping 7 2010-10-01 2010-10-01 false Accommodations. 169.317 Section 169.317 Shipping COAST... and Arrangement Living Spaces § 169.317 Accommodations. (a) Quarters must have sufficient fresh...

  19. Advanced Solar Observatory (ASO) accommodations requirements study

    NASA Technical Reports Server (NTRS)

    1989-01-01

    Results of an accommodations analysis for the Advanced Solar Observatory on Space Station Freedom are reported. Concepts for the High Resolution Telescope Cluster, Pinhole/Occulter Facility, and High Energy Cluster were developed which can be accommodated on Space Station Freedom. It is shown that workable accommodations concepts are possible. Areas of emphasis for the next stage of engineering development are identified.

  20. 46 CFR 108.143 - Accommodation space.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Accommodation space. 108.143 Section 108.143 Shipping... EQUIPMENT Construction and Arrangement Structural Fire Protection § 108.143 Accommodation space. (a) Each corridor bulkhead in an accommodation space must be an A class or B class bulkhead except if an A...

  1. 46 CFR 108.143 - Accommodation space.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Accommodation space. 108.143 Section 108.143 Shipping... EQUIPMENT Construction and Arrangement Structural Fire Protection § 108.143 Accommodation space. (a) Each corridor bulkhead in an accommodation space must be an A class or B class bulkhead except if an A...

  2. 46 CFR 108.143 - Accommodation space.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Accommodation space. 108.143 Section 108.143 Shipping... EQUIPMENT Construction and Arrangement Structural Fire Protection § 108.143 Accommodation space. (a) Each corridor bulkhead in an accommodation space must be an A class or B class bulkhead except if an A...

  3. 46 CFR 108.143 - Accommodation space.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Accommodation space. 108.143 Section 108.143 Shipping... EQUIPMENT Construction and Arrangement Structural Fire Protection § 108.143 Accommodation space. (a) Each corridor bulkhead in an accommodation space must be an A class or B class bulkhead except if an A...

  4. 46 CFR 108.143 - Accommodation space.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Accommodation space. 108.143 Section 108.143 Shipping... EQUIPMENT Construction and Arrangement Structural Fire Protection § 108.143 Accommodation space. (a) Each corridor bulkhead in an accommodation space must be an A class or B class bulkhead except if an A...

  5. Soyuz/ACRV accommodation study

    NASA Technical Reports Server (NTRS)

    Cruz, Jonathan; Gould, Marston J.; Dahlstrom, Eric

    1993-01-01

    Included is a set of viewgraphs that present the results of a study conducted at the LaRC Space Station Freedom Office at the request of the Space Station Freedom Level 1 Program Office and the JSC ACRV Project Office to determine the implications of accommodating two Soyuz TM spacecraft as Assured Crew Return Vehicles (ACRV) on the Space Station Freedom (SSF) at the Permanently Crewed Capability (PCC) stage. The study examined operational as well as system issues associated with the accommodation of the Soyuz for several potential configuration options. Operational issues considered include physical hardware clearances, worst case Soyuz departure paths, and impacts to baseline operations such as Pressurized Logistics Module (PLM) exchange, Space Station Remote Manipulator System (SSRMS) attachment, Extravehicular Activity (EVA), and automatic rendezvous and docking (AR&D). Systems impact analysis included determining differences between Soyuz interface requirements and SSF capabilities for the Electrical Power System (EPS), Thermal Control System (TCS), Communications and Tracking (C&T), Audio-Video Subsystem (A/V), Data Management System (DMS), and Environmental Control and Life Support System (ECLSS). Significant findings of this study have indicated that the current AV capability of the Soyuz will need to be increased to provide adequate departure clearances for a worst case escape from an uncontrolled SSF and that an interface element will be required to mate the Soyuz vehicles to station, provide for AR&D structural loads, and to house Soyuz-to-SSF system interfaces.

  6. Soyuz/ACRV accommodation study

    NASA Astrophysics Data System (ADS)

    Cruz, Jonathan; Gould, Marston J.; Dahlstrom, Eric

    1993-11-01

    Included is a set of viewgraphs that present the results of a study conducted at the LaRC Space Station Freedom Office at the request of the Space Station Freedom Level 1 Program Office and the JSC ACRV Project Office to determine the implications of accommodating two Soyuz TM spacecraft as Assured Crew Return Vehicles (ACRV) on the Space Station Freedom (SSF) at the Permanently Crewed Capability (PCC) stage. The study examined operational as well as system issues associated with the accommodation of the Soyuz for several potential configuration options. Operational issues considered include physical hardware clearances, worst case Soyuz departure paths, and impacts to baseline operations such as Pressurized Logistics Module (PLM) exchange, Space Station Remote Manipulator System (SSRMS) attachment, Extravehicular Activity (EVA), and automatic rendezvous and docking (AR&D). Systems impact analysis included determining differences between Soyuz interface requirements and SSF capabilities for the Electrical Power System (EPS), Thermal Control System (TCS), Communications and Tracking (C&T), Audio-Video Subsystem (A/V), Data Management System (DMS), and Environmental Control and Life Support System (ECLSS). Significant findings of this study have indicated that the current AV capability of the Soyuz will need to be increased to provide adequate departure clearances for a worst case escape from an uncontrolled SSF and that an interface element will be required to mate the Soyuz vehicles to station, provide for AR&D structural loads, and to house Soyuz-to-SSF system interfaces.

  7. Vestibular-ocular accommodation reflex in man

    NASA Technical Reports Server (NTRS)

    Clark, B.; Randle, R. J.; Stewart, J. D.

    1975-01-01

    Stimulation of the vestibular system by angular acceleration produces widespread sensory and motor effects. The present paper studies a motor effect which has not been reported in the literature, i.e., the influence of rotary acceleration of the body on ocular accommodation. The accommodation of 10 young men was recorded before and after a high-level deceleration to zero velocity following 30 sec of rotating. Accommodation was recorded continuously on an infrared optometer for 110 sec under two conditions: while the subjects observed a target set at the far point, and while they viewed the same target through a 0.3-mm pinhole. Stimulation by high-level rotary deceleration produced positive accommodation or a pseudomyopia under both conditions, but the positive accommodation was substantially greater and lasted much longer during fixation through the pinhole. It is hypothesized that this increase in accommodation is a result of a vestibular-ocular accommodation reflex.

  8. Expression of p21WAF1 in Astler-Coller stage B2 colorectal cancer is associated with survival benefit from 5FU-based adjuvant chemotherapy.

    PubMed

    Sulzyc-Bielicka, Violetta; Domagala, Pawel; Urasinska, Elzbieta; Bielicki, Dariusz; Safranow, Krzysztof; Domagala, Wenancjusz

    2011-04-01

    In several, but not all, previous studies, positive p21(WAF1) expression has been suggested as an indicator of a good prognosis in patients with stage III/IV colorectal cancer. However, it is not known whether the same is true for stage B2 patients. The purpose of this study is to assess the influence of p21(WAF1) expression in tumor cells on disease-free survival (DFS) and overall survival (OS) of Astler-Coller stage B2 and C patients with colorectal cancer who underwent 5-fluorouracil-based adjuvant chemotherapy. Nuclear p21(WAF1) was detected by immunohistochemistry in tissue microarrays from 275 colorectal cancers. The expression of p21(WAF1) was associated with DFS (p = 0.025) and OS (p = 0.008) in the subgroup of stage B2 patients that was treated with adjuvant chemotherapy. In multivariate analysis, it remained the only independent prognostic parameter in relation to DFS and OS (p = 0.035 and p = 0.02, respectively). In the subgroup of 72 stage B2 patients with positive p21(WAF1) expression but not in the subgroup of 61 stage B2 patients with negative p21(WAF1) expression, adjuvant chemotherapy was associated with better DFS (85% 5-year survival versus 65% without chemotherapy, p = 0.03) and OS (96% versus 82%, p = 0.014). In the combined stage B2 and C group of patients treated with adjuvant chemotherapy, positive p21(WAF1) expression was also associated with better DFS and OS (p = 0.03, p = 0.002, respectively). Expression of p21(WAF1) in colorectal tumor cells identifies a subgroup of Astler-Coller stage B2 patients who could benefit significantly from 5FU-based chemotherapy and may improve the selection of patients for adjuvant chemotherapy.

  9. Differential regulation of p21 (waf1) protein half-life by DNA damage and Nutlin-3 in p53 wild-type tumors and its therapeutic implications.

    PubMed

    Chang, Li-Ju; Eastman, Alan

    2012-09-01

    DNA damage induces the canonical p53 pathway including elevation of p21 (waf1) resulting in arrest of cell cycle progression. This can protect cells from subsequent Chk1 inhibition. Some p53 wild-type cancer cells such as HCT116 and U2OS exhibit attenuated p21 (waf1) induction upon DNA damage due to translational inhibition, and are incapable of maintaining arrest upon Chk1 inhibition. The purpose of this study was to determine whether this attenuated p21 (waf1) induction also occurred with the non-DNA damaging agent Nutlin-3 which induces p53 by disrupting binding to its negative regulator MDM2. We find that Nutlin-3 circumvented the attenuated induction of p21 (waf1) protein by increasing its half-life which led to G 1 and G 2 arrest in both cell lines. Interestingly, the p21 (waf1) protein half-life remained short on Nutlin-3 in p53 wild-type MCF10A cells; these cells achieve high p21 (waf1) levels through transcriptional upregulation. Consequently, all three p53 wild-type cells but not p53 mutant MDA-MB-231 cancer cells were protected from subsequent incubation with a combination of DNA damage plus a checkpoint inhibitor.

  10. The p21Waf1 pathway is involved in blocking leukemogenesis by the t(8;21) fusion protein AML1-ETO

    PubMed Central

    Peterson, Luke F.; Yan, Ming

    2007-01-01

    The 8;21 translocation is a major contributor to acute myeloid leukemia (AML) of the M2 classification occurring in approximately 40% of these cases. Multiple mouse models using this fusion protein demonstrate that AML1-ETO requires secondary mutagenic events to promote leukemogenesis. Here, we show that the negative cell cycle regulator p21WAF1 gene is up-regulated by AML1-ETO at the protein, RNA, and promoter levels. Retroviral transduction and hematopoietic cell transplantation experiments with p21WAF1-deficient cells show that AML1-ETO is able to promote leukemogenesis in the absence of p21WAF1. Thus, loss of p21WAF1 facilitates AML1-ETO–induced leukemogenesis, suggesting that mutagenic events in the p21WAF1 pathway to bypass the growth inhibitory effect from AML1-ETO–induced p21WAF1 expression can be a significant factor in AML1-ETO–associated acute myeloid leukemia. PMID:17284535

  11. Photodynamic therapy results in induction of WAF1/CIP1/P21 leading to cell cycle arrest and apoptosis.

    PubMed

    Ahmad, N; Feyes, D K; Agarwal, R; Mukhtar, H

    1998-06-09

    Photodynamic therapy (PDT) is a promising new modality that utilizes a combination of a photosensitizing chemical and visible light for the management of a variety of solid malignancies. The mechanism of PDT-mediated cell killing is not well defined. We investigated the involvement of cell cycle regulatory events during silicon phthalocyanine (Pc4)-PDT-mediated apoptosis in human epidermoid carcinoma cells A431. PDT resulted in apoptosis, inhibition of cell growth, and G0-G1 phase arrest of the cell cycle, in a time-dependent fashion. Western blot analysis revealed that PDT results in an induction of the cyclin kinase inhibitor WAF1/CIP1/p21, and a down-regulation of cyclin D1 and cyclin E, and their catalytic subunits cyclin-dependent kinase (cdk) 2 and cdk6. The treatment also resulted in a decrease in kinase activities associated with all the cdks and cyclins examined. PDT also resulted in (i) an increase in the binding of cyclin D1 and cdk6 toward WAF1/CIP1/p21, and (ii) a decrease in the binding of cyclin D1 toward cdk2 and cdk6. The binding of cyclin E and cdk2 toward WAF1/CIP1/p21, and of cyclin E toward cdk2 did not change by the treatment. These data suggest that PDT-mediated induction of WAF1/CIP1/p21 results in an imposition of artificial checkpoint at G1 --> S transition thereby resulting in an arrest of cells in G0-G1 phase of the cell cycle through inhibition in the cdk2, cdk6, cyclin D1, and cyclin E. We suggest that this arrest is an irreversible process and the cells, unable to repair the damages, ultimately undergo apoptosis.

  12. [Current progress of the artificial accommodation system].

    PubMed

    Bretthauer, G; Gengenbach, U; Nagel, J A; Beck, C; Fliedner, J; Koker, L; Krug, M; Martin, T; Stachs, O; Guthoff, R F

    2014-12-01

    In case of presbyopia or cataract the "artificial accommodation system" represents one future possibility to durably restore the ability to accommodate. The work presented describes recent progress in the development of the artificial accommodation system. Major advances were achieved in the fields of the actuator system for the active optics, the pupil near reflex sensor, the communication system, the power supply system as well as in system integration. Beside the technical advances, first trials were performed to implant the artificial accommodation system into animals. These trials showed that the new lens shaped design and the C-shaped haptics are beneficial for implantation and secure fixation of the implant inside the capsular bag.

  13. Phenylbutyric acid induces the cellular senescence through an Akt/p21{sup WAF1} signaling pathway

    SciTech Connect

    Kim, Hag Dong; Jang, Chang-Young; Choe, Jeong Min; Sohn, Jeongwon; Kim, Joon

    2012-06-01

    Highlights: Black-Right-Pointing-Pointer Phenylbutyric acid induces cellular senescence. Black-Right-Pointing-Pointer Phenylbutyric acid activates Akt kinase. Black-Right-Pointing-Pointer The knockdown of PERK also can induce cellular senescence. Black-Right-Pointing-Pointer Akt/p21{sup WAF1} pathway activates in PERK knockdown induced cellular senescence. -- Abstract: It has been well known that three sentinel proteins - PERK, ATF6 and IRE1 - initiate the unfolded protein response (UPR) in the presence of misfolded or unfolded proteins in the ER. Recent studies have demonstrated that upregulation of UPR in cancer cells is required to survive and proliferate. Here, we showed that long exposure to 4-phenylbutyric acid (PBA), a chemical chaperone that can reduce retention of unfolded and misfolded proteins in ER, induced cellular senescence in cancer cells such as MCF7 and HT1080. In addition, we found that treatment with PBA activates Akt, which results in p21{sup WAF1} induction. Interestingly, the depletion of PERK but not ATF6 and IRE1 also induces cellular senescence, which was rescued by additional depletion of Akt. This suggests that Akt pathway is downstream of PERK in PBA induced cellular senescence. Taken together, these results show that PBA induces cellular senescence via activation of the Akt/p21{sup WAF1} pathway by PERK inhibition.

  14. Payload accommodations. Satellite servicing support

    NASA Technical Reports Server (NTRS)

    Lee, Roscoe

    1990-01-01

    The proposed technology studies discussed at the Space Transportation Avionics Symposium in Williamsburg, VA on 7 to 9 November 1989, are discussed. The discussions and findings of the Payload Accommodations Subpanel are also summarized. The major objective of the proposed focused technology development is to develop and demonstrate (ground and flight) autonomous rendezvous, proximity operations, and docking/berthing capabilities to support satellite servicing. It is expected that autonomous rendezvous and docking (AR and D) capabilities will benefit both the users (e.g., satellite developers and operators) and the transportation system developers and operators. AR and D will provide increased availability of rendezvous and docking services by reducing the operational constraints associated with current capabilities. These constraints include specific lighting conditions, continuous space-to-ground communications, and lengthy ground tracking periods. AR and D will provide increased cost efficiency with the potential for reduced propellant expenditures and workloads (flight and/or ground crews). The AR and D operations will be more consistent, allowing more flexibility in the design of the satellite control system and docking/berthing mechanisms.

  15. Arecoline-induced phosphorylated p53 and p21(WAF1) protein expression is dependent on ATM/ATR and phosphatidylinositol-3-kinase in clone-9 cells.

    PubMed

    Chou, Wen-Wen; Guh, Jinn-Yuh; Tsai, Jung-Fa; Hwang, Chi-Ching; Chiou, Shean-Jaw; Chuang, Lea-Yea

    2009-06-01

    Betel-quid use is associated with liver cancer whereas its constituent arecoline is cytotoxic, genotoxic, and induces p53-dependent p21(WAF1) protein expression in Clone-9 cells (rat hepatocytes). The ataxia telangiectasia mutated (ATM)/rad3-related (ATR)-p53-p21(WAF1) and the phosphatidylinositol-3-kinase (PI3K)-mammalian target of rapamycin (mTOR) pathways are involved in the DNA damage response and the pathogenesis of cancers. Thus, we studied the role of ATM/ATR and PI3K in arecoline-induced p53 and p21(WAF1) protein expression in Clone-9 cells. We found that arecoline (0.5 mM) activated the ATM/ATR kinase at 30 min. The arecoline-activated ATM/ATR substrate contained p-p53Ser15. Moreover, arecoline only increased the levels of the p-p53Ser6, p-p53Ser15, and p-p53Ser392 phosphorylated p53 isoforms among the known isoforms. ATM shRNA attenuated arecoline-induced p-p53Ser15 and p21(WAF1) at 24 h. Arecoline (0.5 mM) increased phosphorylation levels of p-AktSer473 and p-mTORSer2448 at 30-60 min. Dominant-negative PI3K plasmids attenuated arecoline-induced p21(WAF1), but not p-p53Ser15, at 24 h. Rapamycin attenuated arecoline-induced phosphrylated p-p53Ser15, but not p21(WAF1), at 24 h. ATM shRNA, but not dominant-negative PI3K plasmids, attenuated arecoline-induced p21(WAF1) gene transcription. We conclude that arecoline activates the ATM/ATR-p53-p21(WAF1) and the PI3K/Akt-mTOR-p53 pathways in Clone-9 cells. Arecoline-induced phosphorylated p-p53Ser15 expression is dependent on ATM whereas arecoline-induced p21(WAF1) protein expression is dependent on ATM and PI3K. Moreover, p21(WAF1) gene is transcriptionally induced by arecoline-activated ATM.

  16. Mechanism for accommodation to cadmium exposure in Escherichia coli B

    SciTech Connect

    Kitchen, J.R. Jr.

    1989-01-01

    All organisms possess, to varying degrees, the ability to adapt to changes in their environment. The extent of this capability can be the determining factor in whether or not an organism survives. The adaptation of the enteric microorganism, Escherichia coli, to the heavy metal cadmium is not the result of a beneficial mutation, and has been termed accommodation. A protein was found that binds to, and appears to be induced by cadmium. The work presented in this thesis is directed at (1) determining the mechanism of accommodation of E.coli to cadmium, and (2) determining the potential role of a putative cadmium binding-protein in accomplishing this accommodation. The presence of three chemically related cadmium-binding proteins, possessing molecular weights of 150,000, 67,000, and 38,000, respectively, was demonstrated. The cadmium-protein bond in the 150 and 67 kDa proteins was stable when boiled in sodium dodecyl sulfate, but was lost in the presence of reducing agents. Evidence was obtained which supported the assertion that the lower molecular weight cadmium-binding proteins were proteolytic or oxidative breakdown products of the larger cadmium-binding proteins. The loss of cadmium-binding activity was time dependent, and appeared to be accelerated by the presence of high salt. To determine if the process of accommodation involved the sequestration of cadmium in the outer cell surface, subcellular fractionation experiments were performed under a variety of post-exposure conditions. The possibility that the cell surface was rendered impermeable to cadmium ions during its recovery was also examined. Neither of these processes was found to be involved in the accommodative response. Indeed, the results of these studies support the concept that E.coli circumvents the presence of internal cadmium by converting it to a form that is no longer toxic to the cell.

  17. 14 CFR 1251.201 - Reasonable accommodation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... reasonable accommodation to the known physical or mental limitations of an otherwise qualified handicapped... hardship on the operation of its program or activity. (b) Reasonable accommodation may include: (1) Making... recipient's programor activity, factors to be considered include: (1) The overall size of the...

  18. 28 CFR 42.511 - Reasonable accommodation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...; POLICIES AND PROCEDURES Nondiscrimination Based on Handicap in Federally Assisted Programs or Activities...) A recipient shall make reasonable accommodation to the known physical or mental limitations of an... the operation of its program or activity. (b) Reasonable accommodation may include making...

  19. 45 CFR 1232.10 - Reasonable accommodation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... SERVICE NONDISCRIMINATION ON BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL... shall make reasonable accommodation to the known physical or mental limitations of an otherwise... accommodation would impose an undue hardship on the operation of its program or activity. (b)...

  20. 45 CFR 1232.10 - Reasonable accommodation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... SERVICE NONDISCRIMINATION ON BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL... shall make reasonable accommodation to the known physical or mental limitations of an otherwise... accommodation would impose an undue hardship on the operation of its program or activity. (b)...

  1. 38 CFR 18.412 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... known physical or mental limitations of a handicapped applicant or employee if such accommodation would... that the accommodation would impose an undue hardship on the operation of its program or activity. (b... impose an undue hardship on the operation of a recipient's program or activity, factors to be...

  2. 46 CFR 177.710 - Overnight accommodations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Overnight accommodations. 177.710 Section 177.710 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) CONSTRUCTION AND ARRANGEMENT Crew Spaces § 177.710 Overnight accommodations....

  3. 50 CFR 260.101 - Lavatory accommodations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... toilet accommodations, including, but not being limited to, running hot water (135 °F. or more) and cold water, soap, and single service towels, shall be provided. Such accommodations shall be in or near... which provides an adequate flow of water for washing hands. (d) Durable signs shall be...

  4. Accommodation Outcomes and the ICF Framework

    ERIC Educational Resources Information Center

    Schreuer, Naomi

    2009-01-01

    Accommodation of the environment and technology is one of the key mediators of adjustment to disability and participation in community. In this article, accommodations are tested empirically as facilitators of return to work and participation, as defined by the "International Classification of Disability, Function, and Health" (ICF) and…

  5. 45 CFR 605.12 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Reasonable accommodation. 605.12 Section 605.12 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION...; and (3) The nature and cost of the accommodation needed. (d) A recipient may not deny any...

  6. 45 CFR 605.12 - Reasonable accommodation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Reasonable accommodation. 605.12 Section 605.12 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION...; and (3) The nature and cost of the accommodation needed. (d) A recipient may not deny any...

  7. 45 CFR 605.12 - Reasonable accommodation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 3 2012-10-01 2012-10-01 false Reasonable accommodation. 605.12 Section 605.12 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION...; and (3) The nature and cost of the accommodation needed. (d) A recipient may not deny any...

  8. 45 CFR 605.12 - Reasonable accommodation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 3 2013-10-01 2013-10-01 false Reasonable accommodation. 605.12 Section 605.12 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION...; and (3) The nature and cost of the accommodation needed. (d) A recipient may not deny any...

  9. 45 CFR 605.12 - Reasonable accommodation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Reasonable accommodation. 605.12 Section 605.12 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION...; and (3) The nature and cost of the accommodation needed. (d) A recipient may not deny any...

  10. Accommodation Requests: Who Is Asking for What?

    ERIC Educational Resources Information Center

    Von Schrader, Sarah; Xu, Xu; Bruyère, Susanne M.

    2014-01-01

    Purpose: Workplace accommodations are central to improving employment outcomes for people with and without disabilities; this study presents national estimates comparing accommodation requests and receipt as reported by individuals with and without disabilities. Method: Estimates are developed from the May 2012 Current Population Survey Disability…

  11. Accommodations: Assisting Students with Disabilities. Third Edition

    ERIC Educational Resources Information Center

    Beech, Marty

    2010-01-01

    This document updates "Accommodations: Assisting Students with Disabilities. A Guide for Educators," published by the Florida Department of Education (FDOE) in 2003. This document is written to assist school district personnel and parents when making decisions about the use of accommodations by students with disabilities in instructional…

  12. Bearing-Mounting Concept Accommodates Thermal Expansion

    NASA Technical Reports Server (NTRS)

    Nespodzany, Robert; Davis, Toren S.

    1995-01-01

    Pins or splines allow radial expansion without slippage. Design concept for mounting rotary bearing accommodates differential thermal expansion between bearing and any structure(s) to which bearing connected. Prevents buildup of thermal stresses by allowing thermal expansion to occur freely but accommodating expansion in such way not to introduce looseness. Pin-in-slot configuration also maintains concentricity.

  13. 50 CFR 260.101 - Lavatory accommodations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... facilities for cleaning and sanitizing utensils and hands, shall be provided. (a) Adequate lavatory and toilet accommodations, including, but not being limited to, running hot water (135 °F. or more) and cold water, soap, and single service towels, shall be provided. Such accommodations shall be in or...

  14. Improving Accommodations Outcomes: Monitoring Instructional and Assessment Accommodations for Students with Disabilities

    ERIC Educational Resources Information Center

    Christensen, Laurene L.; Thurlow, Martha L.; Wang, Ting

    2009-01-01

    This document presents a five-step process for schools, districts, and states to use in monitoring accommodations for instruction and assessment. This document was designed to be a companion to the "Council of Chief State School Officers' Accommodations Manual: How to Select, Administer, and Evaluate Use of Accommodations for Instruction and…

  15. Consequences of Using Testing Accommodations: Student, Teacher, and Parent Perceptions of and Reactions to Testing Accommodations

    ERIC Educational Resources Information Center

    Lang, Sylvia C.; Kumke, Patrick J.; Ray, Corey E.; Cowell, Erin L.; Elliott, Stephen N.; Kratochwill, Thomas R.; Bolt, Daniel M.

    2005-01-01

    This study examined student, parent, and teacher perceptions of the use of testing accommodations and the relationship between student perceptions of testing accommodations and their disability status and grade level. Students with and without disabilities completed math and reading achievement tests with and without accommodations. Students,…

  16. Effect of Phenylephrine on the Accommodative System

    PubMed Central

    Del Águila-Carrasco, Antonio J.; Bernal-Molina, Paula; Ferrer-Blasco, Teresa; López-Gil, Norberto; Montés-Micó, Robert

    2016-01-01

    Accommodation is controlled by the action of the ciliary muscle and mediated primarily by parasympathetic input through postganglionic fibers that originate from neurons in the ciliary and pterygopalatine ganglia. During accommodation the pupil constricts to increase the depth of focus of the eye and improve retinal image quality. Researchers have traditionally faced the challenge of measuring the accommodative properties of the eye through a small pupil and thus have relied on pharmacological agents to dilate the pupil. Achieving pupil dilation (mydriasis) without affecting the accommodative ability of the eye (cycloplegia) could be useful in many clinical and research contexts. Phenylephrine hydrochloride (PHCl) is a sympathomimetic agent that is used clinically to dilate the pupil. Nevertheless, first investigations suggested some loss of functional accommodation in the human eye after PHCl instillation. Subsequent studies, based on different measurement procedures, obtained contradictory conclusions, causing therefore an unexpected controversy that has been spread almost to the present days. This manuscript reviews and summarizes the main research studies that have been performed to analyze the effect of PHCl on the accommodative system and provides clear conclusions that could help clinicians know the real effects of PHCl on the accommodative system of the human eye. PMID:28053778

  17. [Techniques for measuring phakic and pseudophakic accommodation. Methodology for distinguishing between neurological and mechanical accommodative insufficiency].

    PubMed

    Roche, O; Roumes, C; Parsa, C

    2007-11-01

    The methods available for studying accommodation are evaluated: Donder's "push-up" method, dynamic retinoscopy, infrared optometry using the Scheiner principle, and wavefront analysis are each discussed with their inherent advantages and limitations. Based on the methodology described, one can also distinguish between causes of accommodative insufficiency. Dioptric insufficiency (accommodative lag) that remains equal at various testing distances from the subject indicates a sensory/neurologic (afferent), defect, whereas accommodative insufficiency changing with distance indicates a mechanical/restrictive (efferent) defect, such as in presbyopia. Determining accommodative insufficiency and the cause can be particularly useful when examining patients with a variety of diseases associated with reduced accommodative ability (e.g., Down syndrome and cerebral palsy) as well as in evaluating the effectiveness of various potentially accommodating intraocular lens designs.

  18. NSPc1 is a cell growth regulator that acts as a transcriptional repressor of p21Waf1/Cip1 via the RARE element

    PubMed Central

    Gong, Yanhua; Yue, Jiping; Wu, Xudong; Wang, Xu; Wen, Jianyan; Lu, Lifang; Peng, Xiaozhong; Qiang, Boqin; Yuan, Jiangang

    2006-01-01

    The mammalian polycomb group proteins play an important role in cell cycle control and tumorigenesis. Nervous system polycomb 1 (NSPc1) is a newly identified transcription repressor, highly homologous with PcG protein Bmi-1. In this article, we showed that NSPc1 could promote tumor cell cycle progression and cell proliferation. Semi-quantitative RT–PCR showed that NSPc1 did not affect the expression levels of most Cyclin-depentent kinases (CDK) inhibitors except for p21Waf1/Cip1. Repression activity assays, chromatin immunoprecipitation (ChIP) and DNA pulldown assays all verified that NSPc1 represses the expression of p21Waf1/Cip1 by binding to the (−1357 to −1083) region of the p21Waf1/Cip1 promoter in vivo, and the repression effect is dependent on the retinoid acid response element (RARE element) within the above region of the p21Waf1/Cip1 promoter. Further analysis showed that NSPc1 could compete the RARE element site with RA receptors both in vitro and in vivo. Taken together, our results support the hypothesis that NSPc1 has a positive role in tumor cell growth by down-regulating p21Waf1/Cip1 via the RARE element, which directly connects transcriptional repression of PcGs to CDKIs and RA signaling pathways. PMID:17088287

  19. p21Waf1 is required for complete oncogenic transformation of mouse embryo fibroblasts by E1Aad5 and c-Ha-ras oncogenes.

    PubMed

    Romanov, Vasily S; Bardin, Alexander A; Zubova, Svetlana G; Bykova, Tatiana V; Pospelov, Valery A; Pospelova, Tatiana V

    2011-09-01

    Cyclin-dependent kinase inhibitor p21(Waf1) is known to have alternative functions associated with positive regulation of proliferation, actin cytoskeleton remodeling and suppression of apoptosis. The goal of the present study was to assess the role of p21(Waf1) in the establishment of the transformed phenotype of mouse embryo fibroblasts with stable expression of E1Aad5 and c-Ha-ras complementary oncogenes. Herein, we demonstrate that E1A/c-Ha-Ras-transformed p21(Waf1)-null fibroblasts possess some characteristic features of transformed cells, such as loss of contact inhibition, high saturation density, shortened cell cycle, inability to undergo cell-cycle arrest after DNA damage and serum deprivation, but, at the same time, they are not completely transformed in that they are unable to proliferate at clonal density, are anchorage-dependent, retain a fibroblast-like morphology with pronounced actin cytoskeleton and show reduced migration and invasion. Our data support the concept of p21(Waf1) "tumor suppressor" having alternative oncogenic functions in the cytoplasm and for the first time indicate that p21(Waf1) can be indispensable for complete oncogenic transformation.

  20. 10 CFR 1040.67 - Reasonable accommodation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION IN FEDERALLY ASSISTED PROGRAMS OR ACTIVITIES... known physical or mental limitations of an otherwise qualified handicapped applicant or employee unless... of its program or activity. (b) Reasonable accommodation may include: (1) Making facilities used...

  1. 45 CFR 84.12 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Employment Practices... physical or mental limitations of an otherwise qualified handicapped applicant or employee unless the... program or activity. (b) Reasonable accommodation may include: (1) Making facilities used by...

  2. Accommodation of workers with chronic neurologic disorders.

    PubMed

    Bleecker, Margit L; Barnes, Sheryl K

    2015-01-01

    The ability to work is important to those with chronic neurologic disorders (CND) and to the aging workforce. Many signs and symptoms are similar in those with CND and normal aging, but may interfere with the ability to work if not appropriately accommodated. This requires the healthcare provider to recognize the specific features of the CND that interferes with work and how it can be accommodated. Review of the American with Disabilities Act and the subsequent amendment informs the healthcare provider as to what is covered under the law and how the disability can be accommodated. Overall employers want to retain qualified employees and therefore accommodating workers is beneficial to both the employee with CND and the employer.

  3. 50 CFR 260.101 - Lavatory accommodations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Fishery Products for Human Consumption Requirements for Plants Operating Under Continuous Inspection on A... water, soap, and single service towels, shall be provided. Such accommodations shall be in or...

  4. 50 CFR 260.101 - Lavatory accommodations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Fishery Products for Human Consumption Requirements for Plants Operating Under Continuous Inspection on A... water, soap, and single service towels, shall be provided. Such accommodations shall be in or...

  5. Diabetes-Induced Oxidative DNA Damage Alters p53-p21CIP1/Waf1 Signaling in the Rat Testis

    PubMed Central

    Al-Bader, Maie M.

    2015-01-01

    Diabetes is increasingly becoming a major cause of large-scale morbidity and mortality. Diabetes-induced oxidative stress alters numerous intracellular signaling pathways. Although testicular dysfunction is a major concern in diabetic men, the mechanistic alterations in the testes that lead to hypogonadism are not yet clear. Oxidative mitochondrial DNA damage, as indicated by 7,8-dihydro-8-oxo-2′-deoxyguanosine, and phosphorylation of p53 at ser315 residue (p-p53ser315) increased in a stage- and cell-specific manner in the testes of rats that were diabetic for 1 month (DM1). Prolongation of diabetes for 3 months (DM3) led to an increase in nuclear oxidative DNA damage in conjunction with a decrease in the expression of p-p53ser315. The nuclei of pachytene and preleptotene spermatocytes, steps 1, 11, and 12 spermatids, secondary spermatocytes and the Sertoli cells, and the meiotic figures showed an increase in the expression of p-p53ser315. An increase in the expression of a downstream target of p53 and protein 21cyclin-dependent kinase interacting protein 1/wild-type p53-activated factor 1 (p21CIP1/Waf1) in both diabetic groups did not show any time-dependent effects but occurred concurrent with an upregulation of p-p53ser315 in DM1 and a downregulation of the protein in DM3. In diabetic groups, the expression of p21CIP1/Waf1 was mainly cytoplasmic but also perinuclear in pachytene spermatocytes and round spermatids. The cytoplasmic localization of p21CIP1/Waf1 may be suggestive of an antiapoptotic role for the protein. The perinuclear localization is probably related to the cell cycle arrest meant for DNA damage repair. Diabetes upregulates p21CIP1/Waf1 signaling in testicular germ cells in association with alteration in p-p53ser315 expression, probably to counteract DNA damage-induced cell death. PMID:24828139

  6. Accommodation dynamics in aging rhesus monkeys.

    PubMed

    Croft, M A; Kaufman, P L; Crawford, K S; Neider, M W; Glasser, A; Bito, L Z

    1998-12-01

    Accommodation, the mechanism by which the eye focuses on near objects, is lost with increasing age in humans and monkeys. This pathophysiology, called presbyopia, is poorly understood. We studied aging-related changes in the dynamics of accommodation in rhesus monkeys aged 4-24 yr after total iridectomy and midbrain implantation of an electrode to permit visualization and stimulation, respectively, of the eye's accommodative apparatus. Real-time video techniques were used to capture and quantify images of the ciliary body and lens. During accommodation in youth, ciliary body movement was biphasic, lens movement was monophasic, and both slowed as the structures approached their new steady-state positions. Disaccommodation occurred more rapidly for both ciliary body and lens, but with longer latent period, and slowed near the end point. With increasing age, the amplitude of lens and ciliary body movement during accommodation declined, as did their velocities. The latent period of lens and ciliary body movements increased, and ciliary body movement became monophasic. The latent period of lens and ciliary body movement during disaccommodation was not significantly correlated with age, but their velocity declined significantly. The age-dependent decline in amplitude and velocity of ciliary body movements during accommodation suggests that ciliary body dysfunction plays a role in presbyopia. The age changes in lens movement could be a consequence of increasing inelasticity or hardening of the lens, or of age changes in ciliary body motility.

  7. Modified method of accommodative facility evaluation

    NASA Astrophysics Data System (ADS)

    Kedzia, Boleslaw; Pieczyrak, Danuta; Tondel, Grazyna; Maples, Willis C.

    1998-10-01

    Background: Accommodative facility testing is a common test performed by optometrist to investigate an individuals skill at focusing objects at near and at far. The traditional test however harbors possible confounding variables including individual variance in reaction time, visual acuity, verbal skills and oculomotor function. We have designed a test procedure to control these variables. Methods: Children were evaluated with a traditional accommodative facility test, a test which evaluated reaction time and language skill but without accommodative (plano lenses) and a test which evaluated reaction time, language skill and accommodative facility (+/- 2.00 D lenses). Results: Speed of reaction time was 2.9 sec/cycle for the plano lenses (for dominant eye). Speed of reaction with +/- 2.00 D lenses was 6.6 sec/cycle for dominant eye and the monocular speed of accommodations was calculated to average 3.7 sec/cycle. Normative data reported in the literature was calculated to be 5.5 sec/cycle. Discussion: We found that both our method which controls for confounding variables the traditional method reveal similar findings but that individual subjects would pass one method and fail the other. This is attributed to variation in the reaction time and digit naming skill. Conclusions: Although both methods reap similar results, both methods should be employed to discover, in those who score below the expected finding, to tease out whether or not the fault falls within the reaction time/language area or whether it is a true accommodative facility dysfunction.

  8. Prolonged induction of p21Cip1/WAF1/CDK2/PCNA complex by epidermal growth factor receptor activation mediates ligand-induced A431 cell growth inhibition

    PubMed Central

    1995-01-01

    Proliferation of some cultured human tumor cell lines bearing high numbers of epidermal growth factor (EGF) receptors is paradoxically inhibited by EGF in nanomolar concentrations. In the present study, we have investigated the biochemical mechanism of growth inhibition in A431 human squamous carcinoma cells exposed to exogenous EGF. In parallel, we studied a selected subpopulation, A431-F, which is resistant to EGF-mediated growth inhibition. We observed a marked reduction in cyclin-dependent kinase-2 (CDK2) activity when A431 and A431-F cells were cultured with 20 nM EGF for 4 h. After further continuous exposure of A431 cells to EGF, the CDK2 activity remained at a low level and was accompanied by persistent G1 arrest. In contrast, the early reduced CDK2 activity and G1 accumulation in A431-F cells was only transient. We found that, at early time points (4-8 h), EGF induces p21Cip1/WAF1 mRNA and protein expression in both EGF-sensitive A431 cells and EGF-resistant A431-F cells. But only in A431 cells, was p21Cip1/WAF1 expression sustained at a significantly increased level for up to 5 d after addition of EGF. Induction of p21Cip1/WAF1 by EGF could be inhibited by a specific EGF receptor tyrosine kinase inhibitor, tyrphostin AG1478, suggesting that p21Cip1/WAF1 induction was a consequence of receptor tyrosine kinase activation by EGF. We also demonstrated that the increased p21Cip1/WAF1 was associated with both CDK2 and proliferating cell nuclear antigen (PCNA). Taken together, our results demonstrate that p21Cip1/WAF1 is an important mediator of EGF-induced G1 arrest and growth inhibition in A431 cells. PMID:7559780

  9. Alphavirus M1 induces apoptosis of malignant glioma cells via downregulation and nucleolar translocation of p21WAF1/CIP1 protein.

    PubMed

    Hu, Jun; Cai, Xiao-Feng; Yan, Guangmei

    2009-10-15

    Alphavirus, a genus of arthropod-borne togavirus, is well-known for its pro-apoptotic capability. However, the underlying mechanism remains to be further clarified. Here, we have identified that M1, an alphavirus isolated in 1960s, targeted C6 malignant glioma cells for apoptosis. Flow cytometry analysis showed that more cells enter S-phase post M1 infection, and subsequently undergo a classic apoptosis. To elucidate the mechanism of S-phase arrest and its relationship to apoptosis, we tested the expression of several critical cell cycle regulatory proteins and found elevated phosphorylation of cyclin-dependent kinase 2 (CDK2), decreased expression of cyclin A and proliferating cell nuclear antigen (PCNA). Notably, the protein level of p21(WAF1/CIP1) was downregulated earliest and most effectively among all tested changes of cell cycle regulators, though its mRNA level was strongly upregulated. To evaluate the role of p21(WAF1/CIP1) in S-phase accumulation and subsequent apoptosis, we confirmed that exogenous p21(WAF1/CIP1) overexpression or treatment with roscovitine (a selective chemical inhibitor of CDK2) efficiently protected against apoptosis with a reduced S-phase accumulation. Thus, it is indicated that the downregulation of p21(WAF1/CIP1) mediated C6 apoptosis via overactivation of CDK2. In addition, confocal microscopy showed that p21(WAF1/CIP1) totally translocated to nucleolus during M1-induced C6 apoptosis. Altogether, downregulation and nucleolar translocation of the p21(WAF1/CIP1) protein played an active role in M1-induced C6 apoptosis.

  10. Evidence of gene deletion of p21 (WAF1/CIP1), a cyclin-dependent protein kinase inhibitor, in thyroid carcinomas.

    PubMed Central

    Shi, Y.; Zou, M.; Farid, N. R.; al-Sedairy, S. T.

    1996-01-01

    Eukaryotic cell cycle progression is controlled by a host of cyclin/cyclin-dependent kinases (Cdks), that are themselves regulated by multiple factors, including a group of small cyclin-Cdk inhibitor proteins (p15, p16, p21 and p27). The involvement of Cdk inhibitors in carcinogenesis has been demonstrated by the studies of p16. p53 is frequently mutated in thyroid carcinomas and p21/Waf1 is a downstream effector of p53. It is conceivable that genetic defects of genes downstream in the p53 pathway could also be oncogenic. We, therefore, examined a series of 57 thyroid tumour specimens (eight follicular adenomas and 49 carcinomas) for deletion and point mutation of the p21/Waf1 gene. Three different kinds of deletions ranging from 349 to 450 bp were detected in five papillary carcinoma specimens by reverse transcription-polymerase chain reaction (RT-PCR). All the deletions were involved in the second exon of the p21/Waf1 gene. RT-PCR single strand conformational polymorphism (SSCP) analysis of remaining samples failed to reveal any point mutations in the coding region of the gene, except for a polymorphism at codon 31 (Ser to Arg). Genomic Southern blot analysis did not demonstrate any gene deletion or rearrangement in these samples, indicating abnormal RNA splicing may be involved. Analysis of intron-exon boundary and the coding region of the second exon did not reveal any mutation except for a point mutation (C to G) located 16 bp downstream from the splice donor site of the second intron in three out of five samples with p21/Waf1 deletions. Whether the mutation plays any role in aberrant RNA splicing remains to be determined. Among the five samples with p21/Waf1 gene deletions, none of them simultaneously carried a p53 or retinoblastoma (Rb) gene mutation. No p21/Waf1 abnormality was found in the benign adenomas. Thus, 12.5% (5/40) of thyroid papillary carcinoma specimens harboured p21/Waf1 gene deletions. Our data suggest that p21/Waf1 gene deletion is involved

  11. [Energy and memory efficient calculation of the accommodation demand in the artificial accommodation system].

    PubMed

    Nagel, J A; Beck, C; Harms, H; Stiller, P; Guth, H; Stachs, O; Bretthauer, G

    2010-12-01

    Presbyopia and cataract are gaining more and more importance in the ageing society. Both age-related complaints are accompanied with a loss of the eye's ability to accommodate. A new approach to restore accommodation is the Artificial Accommodation System, an autonomous micro system, which will be implanted into the capsular bag instead of a rigid intraocular lens. The Artificial Accommodation System will, depending on the actual demand for accommodation, autonomously adapt the refractive power of its integrated optical element. One possibility to measure the demand for accommodation non-intrusively is to analyse eye movements. We present an efficient algorithm, based on the CORDIC technique, to calculate the demand for accommodation from magnetic field sensor data. It can be shown that specialised algorithms significantly shorten calculation time without violating precision requirements. Additionally, a communication strategy for the wireless exchange of sensor data between the implants of the left and right eye is introduced. The strategy allows for a one-sided calculation of the demand for accommodation, resulting in an overall reduction of calculation time by 50 %. The presented methods enable autonomous microsystems, such as the Artificial Accommodation System, to save significant amounts of energy, leading to extended autonomous run-times.

  12. p21(WAF1) gene promoter is epigenetically silenced by CTIP2 and SUV39H1.

    PubMed

    Cherrier, T; Suzanne, S; Redel, L; Calao, M; Marban, C; Samah, B; Mukerjee, R; Schwartz, C; Gras, G; Sawaya, B E; Zeichner, S L; Aunis, D; Van Lint, C; Rohr, O

    2009-09-24

    Mainly regulated at the transcriptional level, the cellular cyclin-dependent kinase inhibitor, CDKN1A/p21(WAF1) (p21), is a major cell cycle regulator of the response to DNA damage, senescence and tumor suppression. Here, we report that COUP-TF-interacting protein 2 (CTIP2), recruited to the p21 gene promoter, silenced p21 gene transcription through interactions with histone deacetylases and methyltransferases. Importantly, treatment with the specific SUV39H1 inhibitor, chaetocin, repressed histone H3 lysine 9 trimethylation at the p21 gene promoter, stimulated p21 gene expression and induced cell cycle arrest. In addition, CTIP2 and SUV39H1 were recruited to the silenced p21 gene promoter to cooperatively inhibit p21 gene transcription. Induction of p21(WAF1) gene upon human immunodeficiency virus 1 (HIV-1) infection benefits viral expression in macrophages. Here, we report that CTIP2 further abolishes Vpr-mediated stimulation of p21, thereby indirectly contributing to HIV-1 latency. Altogether, our results suggest that CTIP2 is a constitutive p21 gene suppressor that cooperates with SUV39H1 and histone methylation to silence the p21 gene transcription.

  13. Overview for Attached Payload Accommodations and Environments

    NASA Technical Reports Server (NTRS)

    Schaffer, Craig; Cook, Gene; Nabizadeh, Rodney; Phillion, James

    2007-01-01

    External payload accommodations are provided at attach sites on the U.S provided ELC, U.S. Truss, the Japanese Experiment Module Exposed Facility (JEM EF) and the Columbus EPF (External Payload Facilities). The Integrated Truss Segment (ITS) provides the backbone structure for the ISS. It attaches the solar and thermal control arrays to the rest of the complex, and houses cable distribution trays Extravehicular Activity (EVA) support equipment such as handholds and lighting; and providing for Extravehicular Robotic (EVR) accommodations using the Mobile Servicing System (MSS). It also provides logistics and maintenance, and payload attachment sites. The attachment sites accommodate logistics and maintenance and payloads carriers, zenith and nadir. The JEM-EF, a back porch-like attachment to the JEM Pressurized Module, accommodates up to eight payloads, which can be serviced by the crew via the JEM PM's airlock and dedicated robotic arm. The Columbus-EPF is another porch-like platform that can accommodate two zenith and two nadir looking payloads.

  14. Bio-inspired accommodating fluidic intraocular lens.

    PubMed

    Qiao, Wen; Johnson, Daniel; Tsai, Frank S; Cho, Sung Hwan; Lo, Yu-Hwa

    2009-10-15

    The invention of intraocular lens (IOL), a substitute for crystalline lens, represents a major advancement in cataract surgery. After about sixty years of IOL development, one key remaining problem is its limited accommodation range compared with natural eyes. To overcome this performance limit, we explore bio-inspired fluidic IOL. By mimicking the working principle of natural eyes, a fluidic intraocular lens can achieve an exceedingly large accommodation range. An experiment on fluidic IOL demonstrated a very high tuning range of 12 D. This accommodation range was achieved with a modest amount of force (0.06 N) and equatorial radius change (0.286 mm), in conditions matching well with the characteristics of aged eyes.

  15. Prediction of anthropometric accommodation in aircraft cockpits

    NASA Astrophysics Data System (ADS)

    Zehner, Gregory Franklin

    Designing aircraft cockpits to accommodate the wide range of body sizes existing in the U.S. population has always been a difficult problem for Crewstation Engineers. The approach taken in the design of military aircraft has been to restrict the range of body sizes allowed into flight training, and then to develop standards and specifications to ensure that the majority of the pilots are accommodated. Accommodation in this instance is defined as the ability to: (1) Adequately see, reach, and actuate controls; (2) Have external visual fields so that the pilot can see to land, clear for other aircraft, and perform a wide variety of missions (ground support/attack or air to air combat); and (3) Finally, if problems arise, the pilot has to be able to escape safely. Each of these areas is directly affected by the body size of the pilot. Unfortunately, accommodation problems persist and may get worse. Currently the USAF is considering relaxing body size entrance requirements so that smaller and larger people could become pilots. This will make existing accommodation problems much worse. This dissertation describes a methodology for correcting this problem and demonstrates the method by predicting pilot fit and performance in the USAF T-38A aircraft based on anthropometric data. The methods described can be applied to a variety of design applications where fitting the human operator into a system is a major concern. A systematic approach is described which includes: defining the user population, setting functional requirements that operators must be able to perform, testing the ability of the user population to perform the functional requirements, and developing predictive equations for selecting future users of the system. Also described is a process for the development of new anthropometric design criteria and cockpit design methods that assure body size accommodation is improved in the future.

  16. Experimental investigations of pupil accommodation factors.

    PubMed

    Lee, Eui Chul; Lee, Ji Woo; Park, Kang Ryoung

    2011-08-17

    PURPOSE. The contraction and dilation of the iris muscle that controls the amount of light entering the retina causes pupil accommodation. In this study, experiments were performed and two of the three factors that influence pupil accommodation were analyzed: lighting conditions and depth fixations. The psychological benefits were not examined, because they could not be quantified. METHODS. A head-wearable eyeglasses-based, eye-capturing device was designed to measure pupil size. It included a near-infrared (NIR) camera and an NIR light-emitting diode. Twenty-four subjects watched two-dimensional (2D) and three-dimensional (3D) stereoscopic videos of the same content, and the changes in pupil size were measured by using the eye-capturing device and image-processing methods: RESULTS. The pupil size changed with the intensity of the videos and the disparities between the left and right images of a 3D stereoscopic video. There was correlation between the pupil size and average intensity. The pupil diameter could be estimated as being contracted from approximately 5.96 to 4.25 mm as the intensity varied from 0 to 255. Further, from the changes in the depth fixation for the pupil accommodation, it was confirmed that the depth fixation also affected accommodation of pupil size. CONCLUSIONS. It was confirmed that the lighting condition was an even more significant factor in pupil accommodation than was depth fixation (significance ratio: approximately 3.2:1) when watching 3D stereoscopic video. Pupil accommodation was more affected by depth fixation in the real world than was the binocular convergence in the 3D stereoscopic display.

  17. Aerobrake assembly with minimum Space Station accommodation

    NASA Technical Reports Server (NTRS)

    Katzberg, Steven J.; Butler, David H.; Doggett, William R.; Russell, James W.; Hurban, Theresa

    1991-01-01

    The minimum Space Station Freedom accommodations required for initial assembly, repair, and refurbishment of the Lunar aerobrake were investigated. Baseline Space Station Freedom support services were assumed, as well as reasonable earth-to-orbit possibilities. A set of three aerobrake configurations representative of the major themes in aerobraking were developed. Structural assembly concepts, along with on-orbit assembly and refurbishment scenarios were created. The scenarios were exercised to identify required Space Station Freedom accommodations. Finally, important areas for follow-on study were also identified.

  18. 46 CFR 92.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Sleeping accommodations. 92.20-20 Section 92.20-20... CONSTRUCTION AND ARRANGEMENT Accommodations for Officers and Crew § 92.20-20 Sleeping accommodations. (a) Where practicable, each licensed officer must be provided with a separate stateroom. (b) Sleeping accommodations...

  19. 46 CFR 92.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Sleeping accommodations. 92.20-20 Section 92.20-20... CONSTRUCTION AND ARRANGEMENT Accommodations for Officers and Crew § 92.20-20 Sleeping accommodations. (a) Where practicable, each licensed officer must be provided with a separate stateroom. (b) Sleeping accommodations...

  20. 46 CFR 72.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 3 2011-10-01 2011-10-01 false Sleeping accommodations. 72.20-20 Section 72.20-20... ARRANGEMENT Accommodations for Officers and Crew § 72.20-20 Sleeping accommodations. (a) Where practicable, each licensed officer shall be provided with a separate stateroom. (b) Sleeping accommodations for...

  1. 46 CFR 72.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Sleeping accommodations. 72.20-20 Section 72.20-20... ARRANGEMENT Accommodations for Officers and Crew § 72.20-20 Sleeping accommodations. (a) Where practicable, each licensed officer shall be provided with a separate stateroom. (b) Sleeping accommodations for...

  2. 28 CFR 36.310 - Transportation provided by public accommodations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... accommodations. 36.310 Section 36.310 Judicial Administration DEPARTMENT OF JUSTICE NONDISCRIMINATION ON THE BASIS OF DISABILITY BY PUBLIC ACCOMMODATIONS AND IN COMMERCIAL FACILITIES Specific Requirements § 36.310 Transportation provided by public accommodations. (a) General. (1) A public accommodation that...

  3. 46 CFR 108.195 - Location of accommodation spaces.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Location of accommodation spaces. 108.195 Section 108... DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.195 Location of accommodation spaces. (a) On surface type units, accommodation spaces must not be located forward of a...

  4. 46 CFR 108.195 - Location of accommodation spaces.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Location of accommodation spaces. 108.195 Section 108... DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.195 Location of accommodation spaces. (a) On surface type units, accommodation spaces must not be located forward of a...

  5. 46 CFR 108.195 - Location of accommodation spaces.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Location of accommodation spaces. 108.195 Section 108... DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.195 Location of accommodation spaces. (a) On surface type units, accommodation spaces must not be located forward of a...

  6. 46 CFR 92.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Sleeping accommodations. 92.20-20 Section 92.20-20... CONSTRUCTION AND ARRANGEMENT Accommodations for Officers and Crew § 92.20-20 Sleeping accommodations. (a) Where practicable, each licensed officer must be provided with a separate stateroom. (b) Sleeping accommodations...

  7. 46 CFR 72.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 3 2014-10-01 2014-10-01 false Sleeping accommodations. 72.20-20 Section 72.20-20... ARRANGEMENT Accommodations for Officers and Crew § 72.20-20 Sleeping accommodations. (a) Where practicable, each licensed officer shall be provided with a separate stateroom. (b) Sleeping accommodations for...

  8. 46 CFR 72.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 3 2013-10-01 2013-10-01 false Sleeping accommodations. 72.20-20 Section 72.20-20... ARRANGEMENT Accommodations for Officers and Crew § 72.20-20 Sleeping accommodations. (a) Where practicable, each licensed officer shall be provided with a separate stateroom. (b) Sleeping accommodations for...

  9. 46 CFR 72.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 3 2012-10-01 2012-10-01 false Sleeping accommodations. 72.20-20 Section 72.20-20... ARRANGEMENT Accommodations for Officers and Crew § 72.20-20 Sleeping accommodations. (a) Where practicable, each licensed officer shall be provided with a separate stateroom. (b) Sleeping accommodations for...

  10. 46 CFR 92.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Sleeping accommodations. 92.20-20 Section 92.20-20... CONSTRUCTION AND ARRANGEMENT Accommodations for Officers and Crew § 92.20-20 Sleeping accommodations. (a) Where practicable, each licensed officer must be provided with a separate stateroom. (b) Sleeping accommodations...

  11. 46 CFR 92.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Sleeping accommodations. 92.20-20 Section 92.20-20... CONSTRUCTION AND ARRANGEMENT Accommodations for Officers and Crew § 92.20-20 Sleeping accommodations. (a) Where practicable, each licensed officer must be provided with a separate stateroom. (b) Sleeping accommodations...

  12. 46 CFR 108.195 - Location of accommodation spaces.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Location of accommodation spaces. 108.195 Section 108... DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.195 Location of accommodation spaces. (a) On surface type units, accommodation spaces must not be located forward of a...

  13. 46 CFR 108.195 - Location of accommodation spaces.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Location of accommodation spaces. 108.195 Section 108... DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.195 Location of accommodation spaces. (a) On surface type units, accommodation spaces must not be located forward of a...

  14. 15 CFR 8b.12 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... physical or metal limitations of an otherwise qualified handicapped applicant or employee unless the... program or activity. (b) Reasonable accommodation may include: (1) Making the facilities used by the employees in the area where the program or activity is conducted, including common areas used by...

  15. Payload accommodations: Avionics payload support architecture

    NASA Technical Reports Server (NTRS)

    1990-01-01

    Technology issues/trade studies; candidate programs; key contacts/facilities: milestones; accomplishments; and major objectives of the payload accommodation are outlined. Topics covered include: avionics payload support architecture; satellite serving; P/L deploy systems and advanced manipulators; advanced telemetry systems; and on-board abort planning. This presentation is represented by viewgraphs only.

  16. Accommodating Workers with Spinal Cord Injury.

    ERIC Educational Resources Information Center

    Dowler, Denetta; Batiste, Linda; Whidden, Eddie

    1998-01-01

    Examination of over 1,000 calls to the Job Accommodation Network involving workers with spinal cord injury identified the nature of the industry, job, career progression, and accessibility solutions. The number of calls increased dramatically after passage of the Americans with Disabilities Act. (SK)

  17. Anthropometric Accommodation in Space Suit Design

    NASA Technical Reports Server (NTRS)

    Rajulu, Sudhakar; Thaxton, Sherry

    2007-01-01

    Design requirements for next generation hardware are in process at NASA. Anthropometry requirements are given in terms of minimum and maximum sizes for critical dimensions that hardware must accommodate. These dimensions drive vehicle design and suit design, and implicitly have an effect on crew selection and participation. At this stage in the process, stakeholders such as cockpit and suit designers were asked to provide lists of dimensions that will be critical for their design. In addition, they were asked to provide technically feasible minimum and maximum ranges for these dimensions. Using an adjusted 1988 Anthropometric Survey of U.S. Army (ANSUR) database to represent a future astronaut population, the accommodation ranges provided by the suit critical dimensions were calculated. This project involved participation from the Anthropometry and Biomechanics facility (ABF) as well as suit designers, with suit designers providing expertise about feasible hardware dimensions and the ABF providing accommodation analysis. The initial analysis provided the suit design team with the accommodation levels associated with the critical dimensions provided early in the study. Additional outcomes will include a comparison of principal components analysis as an alternate method for anthropometric analysis.

  18. 34 CFR 104.12 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false Reasonable accommodation. 104.12 Section 104.12 Education Regulations of the Offices of the Department of Education OFFICE FOR CIVIL RIGHTS, DEPARTMENT OF EDUCATION NONDISCRIMINATION ON THE BASIS OF HANDICAP IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL...

  19. Accommodating Presuppositions Is Inappropriate in Implausible Contexts

    ERIC Educational Resources Information Center

    Singh, Raj; Fedorenko, Evelina; Mahowald, Kyle; Gibson, Edward

    2016-01-01

    According to one view of linguistic information (Karttunen, 1974; Stalnaker, 1974), a speaker can convey contextually new information in one of two ways: (a) by "asserting" the content as new information; or (b) by "presupposing" the content as given information which would then have to be "accommodated." This…

  20. 24 CFR 8.11 - Reasonable accommodation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Reasonable accommodation. 8.11 Section 8.11 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development NONDISCRIMINATION BASED ON HANDICAP IN FEDERALLY ASSISTED PROGRAMS AND ACTIVITIES OF...

  1. 24 CFR 8.11 - Reasonable accommodation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Reasonable accommodation. 8.11 Section 8.11 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development NONDISCRIMINATION BASED ON HANDICAP IN FEDERALLY ASSISTED PROGRAMS AND ACTIVITIES OF...

  2. 24 CFR 8.11 - Reasonable accommodation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Reasonable accommodation. 8.11 Section 8.11 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development NONDISCRIMINATION BASED ON HANDICAP IN FEDERALLY ASSISTED PROGRAMS AND ACTIVITIES OF...

  3. 24 CFR 8.11 - Reasonable accommodation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Reasonable accommodation. 8.11 Section 8.11 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development NONDISCRIMINATION BASED ON HANDICAP IN FEDERALLY ASSISTED PROGRAMS AND ACTIVITIES OF...

  4. 22 CFR 217.12 - Reasonable accommodation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Reasonable accommodation. 217.12 Section 217.12 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT NONDISCRIMINATION ON THE BASIS OF HANDICAP IN..., acquisition or modification of equipment or devices, the provision of readers or interpreters, and...

  5. 24 CFR 8.11 - Reasonable accommodation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Reasonable accommodation. 8.11 Section 8.11 Housing and Urban Development Office of the Secretary, Department of Housing and Urban Development NONDISCRIMINATION BASED ON HANDICAP IN FEDERALLY ASSISTED PROGRAMS AND ACTIVITIES OF...

  6. 22 CFR 217.12 - Reasonable accommodation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Reasonable accommodation. 217.12 Section 217.12 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT NONDISCRIMINATION ON THE BASIS OF HANDICAP IN..., acquisition or modification of equipment or devices, the provision of readers or interpreters, and...

  7. 22 CFR 217.12 - Reasonable accommodation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Reasonable accommodation. 217.12 Section 217.12 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT NONDISCRIMINATION ON THE BASIS OF HANDICAP IN..., acquisition or modification of equipment or devices, the provision of readers or interpreters, and...

  8. ISS truss attached payload accommodations overview

    NASA Astrophysics Data System (ADS)

    Youmans, Janella S.; Olson, Michael F.; Foster, Mark A.; Watkins, Barbara S.

    1999-01-01

    One of the defining features of the International Space Station (ISS) is its capacity to accommodate long-term science in the external environment of space. The large truss structure spanning the vehicle is designed to support core system equipment such as solar arrays, thermal radiators, and the pressurized module structures. In addition to supporting core systems, the truss structure also accommodates four attached payload facilities and two logistics carriers. This paper focuses on the capabilities of the ISS in accommodating externally attached science payloads, defines the locations where experiments can be conducted, explains the environment wherein typical experiments will be performed, and identifies the payload interfaces and access to resources such as power and data. The paper will also summarize the robotic accommodations which will support attached payloads and describes typical procedures for installation of the payloads onto the sites. Finally, the paper will provide a summary description of the attach sites on the NASDA Exposed Facility and the potential for use of alternative attach sites on the ISS.

  9. College Students' Preferences for Test Accommodations

    ERIC Educational Resources Information Center

    Lewandowski, Lawrence; Lambert, Tonya L.; Lovett, Benjamin J.; Panahon, Carlos J.; Sytsma, Marcia R.

    2014-01-01

    College students with (n = 137) and without disabilities (n = 475) were surveyed about their perceptions of using various types of test accommodations. Results indicated that extended time was perceived as having a positive effect by the most students (>87% of both groups), followed by separate room testing and extra breaks (>60% of both…

  10. 46 CFR 169.317 - Accommodations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Accommodations. 169.317 Section 169.317 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Construction... and noise. (d) Each person on board must have a separate berth which is of sufficient size...

  11. 46 CFR 169.317 - Accommodations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Accommodations. 169.317 Section 169.317 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Construction... and noise. (d) Each person on board must have a separate berth which is of sufficient size...

  12. 46 CFR 169.317 - Accommodations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Accommodations. 169.317 Section 169.317 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Construction... and noise. (d) Each person on board must have a separate berth which is of sufficient size...

  13. 46 CFR 169.317 - Accommodations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Accommodations. 169.317 Section 169.317 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Construction... and noise. (d) Each person on board must have a separate berth which is of sufficient size...

  14. Cultural Accommodation as Method and Metaphor

    ERIC Educational Resources Information Center

    Leong, Frederick T. L.

    2007-01-01

    The author summarizes the cultural accommodation model (CAM) of cross-cultural psychotherapy (F. T. L. Leong & S. H. Lee, 2006). This summary is divided into 2 parts, with the 1st part describing the theoretical development of the CAM as a method of psychotherapy and the research approach underlying it. This section includes a description of the…

  15. Examination Accommodations for Students with Sensory Defensiveness

    ERIC Educational Resources Information Center

    Lewis, Kieran; Nolan, Clodagh

    2013-01-01

    Traditional examination accommodations include extra time, scribes, and/or separate venues for students with disabilities, which have been proven to be successful for the majority of students. For students with non-apparent disabilities such as sensory defensiveness, where sensitivity to a range of sensory information from the environment can…

  16. 10 CFR 4.123 - Reasonable accommodation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Reasonable accommodation. 4.123 Section 4.123 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION IN FEDERALLY ASSISTED PROGRAMS OR ACTIVITIES RECEIVING... composition and structure of the recipient's workforce; and (3) The nature and cost of the...

  17. 50 CFR 260.101 - Lavatory accommodations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Lavatory accommodations. 260.101 Section 260.101 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC... Fishery Products for Human Consumption Requirements for Plants Operating Under Continuous Inspection on...

  18. Accommodating Student Diversity in Remote Sensing Instruction.

    ERIC Educational Resources Information Center

    Hammen, John L., III.

    1992-01-01

    Discusses the difficulty of teaching computer-based remote sensing to students of varying levels of computer literacy. Suggests an instructional method that accommodates all levels of technical expertise through the use of microcomputers. Presents a curriculum that includes an introduction to remote sensing, digital image processing, and…

  19. Accommodating Faculty Members Who Have Disabilities. Report

    ERIC Educational Resources Information Center

    American Association of University Professors, 2012

    2012-01-01

    In recent years the rights and responsibilities of students who have disabilities have received considerable attention. Professors routinely accommodate students with a front-row seat in class or extended time on an examination. Faculty members who have disabilities have received far less attention. This report from a subcommittee of Committee A…

  20. 9 CFR 354.225 - Lavatory accommodations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... for cleaning utensils and hands shall be provided. (a) Adequate lavatory and toilet accommodations, including, but not being limited to, running hot water and cold water, soap, and towels, shall be provided... water for washing hands. (d) Durable signs shall be posted conspicuously in each toilet room and...

  1. 9 CFR 354.225 - Lavatory accommodations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... for cleaning utensils and hands shall be provided. (a) Adequate lavatory and toilet accommodations, including, but not being limited to, running hot water and cold water, soap, and towels, shall be provided... water for washing hands. (d) Durable signs shall be posted conspicuously in each toilet room and...

  2. Accommodating Band Students with Visual Impairments

    ERIC Educational Resources Information Center

    Coates, Rick Lee

    2012-01-01

    This article offers a discussion about some of the accommodations and modifications used in music instruction. The focus here is on the musical tasks and challenges faced by band students with visual impairments. Research and literature reveal an interest in the topic but a lack of accessible materials for immediate use in the classroom and…

  3. Unflagged SATs: Who Benefits from Special Accommodations?

    ERIC Educational Resources Information Center

    Abrams, Samuel J.

    2005-01-01

    When the College Board announced, in the summer of 2002, that it would stop "flagging" the test scores of students who were given special accommodations for the SAT, the gold standard exam for college admission, disability advocates were thrilled. "A triumphant day for millions of people with dyslexia and other disabilities,"…

  4. 29 CFR 32.13 - Reasonable accommodation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... RECEIVING FEDERAL FINANCIAL ASSISTANCE Employment Practices and Employment Related Training Participation... physical or mental limitations of an otherwise qualified handicapped applicant, employee or participant... workforce, and duration and type of training; and (3) The nature and cost of the accommodation needed. (c)...

  5. Water Accommodation on Bare and Coated Ice

    NASA Astrophysics Data System (ADS)

    Kong, Xiangrui

    2015-04-01

    A good understanding of water accommodation on ice surfaces is essential for quantitatively predicting the evolution of clouds, and therefore influences the effectiveness of climate models. However, the accommodation coefficient is poorly constrained within the literature where reported values vary by up to three orders of magnitude. In addition, the complexity of the chemical composition of the atmosphere plays an important role in ice phase behavior and dynamics. We employ an environmental molecular beam (EMB) technique to investigate molecular water interactions with bare and impurity coated ice at temperatures from 170 K to 200 K. In this work, we summarize results of water accommodation experiments on bare ice (Kong et al., 2014) and on ice coated by methanol (Thomson et al., 2013), butanol (Thomson et al., 2013) and acetic acid (Papagiannakopoulos et al., 2014), and compare those results with analogous experiments using hexanol and nitric acid coatings. Hexanol is chosen as a complementary chain alcohol to methanol and butanol, while nitric acid is a common inorganic compound in the atmosphere. The results show a strong negative temperature dependence of water accommodation on bare ice, which can be quantitatively described by a precursor model. Acidic adlayers tend to enhance water uptake indicating that the system kinetics are thoroughly changed compared to bare ice. Adsorbed alcohols influence the temperature dependence of the accommodation coefficient and water molecules generally spend less time on the surfaces before desorbing, although the measured accommodation coefficients remain high and comparable to bare ice for the investigated systems. We conclude that impurities can either enhance or restrict water uptake in ways that are influenced by several factors including temperature and type of adsorbant, with potential implications for the description of ice particle growth in the atmosphere. This work was supported by the Swedish Research Council and

  6. Fractional randomness

    NASA Astrophysics Data System (ADS)

    Tapiero, Charles S.; Vallois, Pierre

    2016-11-01

    The premise of this paper is that a fractional probability distribution is based on fractional operators and the fractional (Hurst) index used that alters the classical setting of random variables. For example, a random variable defined by its density function might not have a fractional density function defined in its conventional sense. Practically, it implies that a distribution's granularity defined by a fractional kernel may have properties that differ due to the fractional index used and the fractional calculus applied to define it. The purpose of this paper is to consider an application of fractional calculus to define the fractional density function of a random variable. In addition, we provide and prove a number of results, defining the functional forms of these distributions as well as their existence. In particular, we define fractional probability distributions for increasing and decreasing functions that are right continuous. Examples are used to motivate the usefulness of a statistical approach to fractional calculus and its application to economic and financial problems. In conclusion, this paper is a preliminary attempt to construct statistical fractional models. Due to the breadth and the extent of such problems, this paper may be considered as an initial attempt to do so.

  7. Involvement of p21cip1/waf1 in the anti-proliferative effects of polyethylene glycol in colon carcinogenesis.

    PubMed

    Roy, Hemant K; Koetsier, Jennifer L; Tiwari, Ashish K; Joshi, Suhasini; Kunte, Dhananjay P; Ward, Tina P; Gandhi, Seema R; Wali, Ramesh K

    2011-02-01

    Polyethylene glycol (PEG) is a safe and effective chemopreventive agent against colorectal carcinogenesis in cell culture, animal models and human subjects. Although the precise molecular mechanism is unclear, we previously reported that PEG suppresses colonic epithelial proliferation. As cellular proliferation is driven by complex G1-S phase transition, we now characterize the role of PEG on cell cycle regulation. We focused our attention on the effect of PEG on the CDK inhibitor p21cip1/waf1, which is implicated in early colon carcinogenesis and is upregulated by non-steroidal anti-inflammatory drugs. These studies were done in the azoxymethane-treated (AOM) rat model as well as in HT-29 colon cancer cells. Immunohistochemical analysis revealed that while AOM decreased the p21 expression (75%, p<0.01) in the premalignant colonic mucosa, PEG induced p21 levels back to normal. These findings paralleled a decreased BrdUrd incorporation (78%, p<0.001) and hypophosphorylated retinoblastoma protein (Rb; by 47%) signifying PEG's antiproliferative activity. Furthermore, in HT-29 cells, PEG decreased proliferation as measured by PCNA (68% reduction), increased p21 expression (2.3-fold), induced cell cycle arrest during G0/G1 phase (45% reduction in S phase cells) and inhibited the phosphorylation of Rb (by 52% compared to untreated). PEG caused greater than a 2-fold induction of protein and mRNA level of p21cip1/waf1 in HT-29 cells. These results demonstrate for the first time that PEG is involved in p21 regulation concomitant with G1S phase cell cycle arrest and it is through these effects that it can exert its anti-proliferative and hence chemopreventive role.

  8. Convoluted accommodation structures in folded rocks

    NASA Astrophysics Data System (ADS)

    Dodwell, T. J.; Hunt, G. W.

    2012-10-01

    A simplified variational model for the formation of convoluted accommodation structures, as seen in the hinge zones of larger-scale geological folds, is presented. The model encapsulates some important and intriguing nonlinear features, notably: infinite critical loads, formation of plastic hinges, and buckling on different length-scales. An inextensible elastic beam is forced by uniform overburden pressure and axial load into a V-shaped geometry dictated by formation of a plastic hinge. Using variational methods developed by Dodwell et al., upon which this paper leans heavily, energy minimisation leads to representation as a fourth-order nonlinear differential equation with free boundary conditions. Equilibrium solutions are found using numerical shooting techniques. Under the Maxwell stability criterion, it is recognised that global energy minimisers can exist with convoluted physical shapes. For such solutions, parallels can be drawn with some of the accommodation structures seen in exposed escarpments of real geological folds.

  9. Shear accommodation in dirty grain boundaries

    NASA Astrophysics Data System (ADS)

    Wang, C.; Upmanyu, M.

    2014-04-01

    The effect of solutes (dirt) on the mechanics of crystalline interfaces remains unexplored. Here, we perform atomic-scale simulations to study the effect of carbon segregation on the shear accommodation at select grain boundaries in the classical α-Fe/C system. For shear velocities larger than the solute diffusion rate, we observe a transition from coupled motion to sliding. Below a critical solute excess, the boundaries break away from the solute cloud and exhibit in a coupled motion. At smaller shear velocities, the extrinsic coupled motion is jerky, occurs at relatively small shear stresses, and is aided by fast convective solute diffusion along the boundary. Our studies underscore the combined effect of energetics and kinetics of solutes in modifying the bicrystallography, temperature and rate dependence of shear accommodation at grain boundaries.

  10. Design Principles to Accommodate Older Adults

    PubMed Central

    Farage, Miranda A.; Miller, Kenneth W.; Ajayi, Funmi; Hutchins, Deborah

    2012-01-01

    The global population is aging. In many industrial countries, almost one in five people are over age 65. As people age, gradual changes ensue in vision, hearing, balance, coordination, and memory. Products, communication materials, and the physical environment must be thoughtfully designed to meet the needs of people of all ages. This article summarizes normal changes in sensory function, mobility, balance, memory, and attention that occur with age. It presents practical guidelines that allow design professionals to accommodate these changes and better meet the needs of older adults. Designing for older adults is inclusive design: it accommodates a range of physical and cognitive abilities and promotes simplicity, flexibility, and ease of use for people of any age. PMID:22980147

  11. Design principles to accommodate older adults.

    PubMed

    Farage, Miranda A; Miller, Kenneth W; Ajayi, Funmi; Hutchins, Deborah

    2012-02-29

    The global population is aging. In many industrial countries, almost one in five people are over age 65. As people age, gradual changes ensue in vision, hearing, balance, coordination, and memory. Products, communication materials, and the physical environment must be thoughtfully designed to meet the needs of people of all ages. This article summarizes normal changes in sensory function, mobility, balance, memory, and attention that occur with age. It presents practical guidelines that allow design professionals to accommodate these changes and better meet the needs of older adults. Designing for older adults is inclusive design: it accommodates a range of physical and cognitive abilities and promotes simplicity, flexibility, and ease of use for people of any age.

  12. Induction of p21(Waf1/Cip1) by garcinol via downregulation of p38-MAPK signaling in p53-independent H1299 lung cancer.

    PubMed

    Yu, Sheng-Yung; Liao, Chiung-Ho; Chien, Ming-Hsien; Tsai, Tsung-Yu; Lin, Jen-Kun; Weng, Meng-Shih

    2014-03-05

    Garcinol, a polyisoprenylated benzophenone, from Garcinia indica fruit rind has possessed anti-inflammatory, antioxidant, antiproliferation, and anticancer activities. However, the anticancer mechanisms of garcinol in lung cancer were still unclear. Therefore, we examine the effects of garcinol on antiproliferation in human lung cancer cells. Treatments with garcinol for 24 h exhibited morphological changes and inhibited the proliferation of H460 (p53-wild type) and H1299 (p53-null) cells in dose- and time-dependent manners. Furthermore, a significant G1 cell cycle arrest was observed in a dose-dependent treatment after H1299 cells were exposed in garcinol, whereas garcinol induced apoptosis rather than cell cycle arrest in H460 cells. Moreover, cyclin-dependent kinase 2 (CDK2), cyclin-dependent kinase 4 (CDK4), cyclin D1, and cyclin D3 were decreased, although cyclin E and cyclin-dependent kinase 6 (CDK6) were increased in garcinol-treated H1299 cells. Meanwhile, the protein levels of CDK inhibitors p21(Waf1/Cip1) and p27(KIP1) also exhibited upregulation after garcinol treatments. The enhanced protein-associated level between p21(Waf1/Cip1) and CDK4/2 rather than p27(KIP1) and CDK4/2 was demonstrated in garcinol-treated cells. Additionally, knock-down p21(Waf1/Cip1) by specific siRNA competently prevented garcinol-induced G1 arrest. Besides, garcinol also inhibited ERK and p38-MAPK activations in time-dependent mode. The pretreatment with p38-MAPK inhibitor but not ERK inhibitor raised garcinol-induced G1 population cells. Co-treatment with p38-MAPK inhibitor and garcinol synergistically elevated cyclin E, p21(Waf1/Cip1), and p27(Kip1) expressions. Meanwhile, overexpression dominant negative p38-MAPK also enhanced garcinol-induced p21(Waf1/Cip1) expression in H1299 cells. Accordingly, our data suggested that garcinol induced G1 cell cycle arrest and apoptosis in lung cancer cells under different p53 statuses. The p53-independent G1 cell cycle arrest induced by

  13. P21waf-1/cip-1/sdi-1 is expressed at G1 phase in primary culture of hepatocytes from old rats, presumably preventing the cells from entering the S phase of the cell cycle.

    PubMed

    Sawada, N; Kojima, T; Obata, H; Saitoh, M; Isomura, H; Kokai, Y; Satoh, M; Mori, M

    1996-11-21

    To elucidate whether p21waf-1/cip-1/sdi-1 expression is associated with loss of growth potential of hepatocytes of old rats, we determined p21waf-1/cip-1/sdi-1 expression of hepatocytes from old (30 months) rats during the cell cycle in primary culture. A high level of expression of p21waf-1/cip-1/sdi-1 was detected at the G1 phase in old-rat hepatocytes, but after the S phase in young-rat hepatocytes. Consistently, the incidence of the cells positive for p21waf-1/cip-1/sdi-1 in nuclei before entering the S phase was significantly higher in old-rat hepatocytes than in young-rat hepatocytes. These results account for the loss of growth potential of old-rat hepatocytes in vitro and the marked retardation of regeneration of liver in old rats in vivo.

  14. ERK mediates anti-apoptotic effect through phosphorylation and cytoplasmic localization of p21Waf1/Cip1/Sdi in response to DNA damage in normal human embryonic fibroblast (HEF) cells.

    PubMed

    Heo, Jee-In; Oh, Soo-Jin; Kho, Yoon-Jung; Kim, Jeong-Hyeon; Kang, Hong-Joon; Park, Seong-Hoon; Kim, Hyun-Seok; Shin, Jong-Yeon; Kim, Min-Ju; Kim, Sung Chan; Park, Jae-Bong; Kim, Jaebong; Lee, Jae-Yong

    2011-04-01

    Since anti-apoptotic effect of ERK has not been elucidated clearly in DNA-damage-induced cell death, the role of ERK was examined in normal HEF cells treated with mild DNA damage using etoposide or camptothecin. ERK was activated by DNA damage in HEF cells. PD98059 increased apoptosis and reduced DNA-damage-induced p21Waf1/Cip1/Sdi level. Depletion of p21Waf1/Cip1/Sdi induced cell death and PD98059 induced additional cell death. DNA-damage-induced increase in cytoplasmic localization and phosphorylation of threonine residues of p21Waf1/Cip1/Sdi was reversed by PD98059. Thus, the results suggest that ERK pathway mediates anti-apoptotic effects through phosphorylation and cytoplasmic localization of p21Waf1/Cip1/Sdi in response to mild DNA damage.

  15. 28 CFR 36.310 - Transportation provided by public accommodations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... public accommodation, customer shuttle bus services operated by private companies and shopping centers, student transportation systems, and transportation provided within recreational facilities such as... readily achievable. (c) Requirements for vehicles and systems. A public accommodation subject to...

  16. 28 CFR 36.310 - Transportation provided by public accommodations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... public accommodation, customer shuttle bus services operated by private companies and shopping centers, student transportation systems, and transportation provided within recreational facilities such as... readily achievable. (c) Requirements for vehicles and systems. A public accommodation subject to...

  17. 28 CFR 36.310 - Transportation provided by public accommodations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... public accommodation, customer shuttle bus services operated by private companies and shopping centers, student transportation systems, and transportation provided within recreational facilities such as... readily achievable. (c) Requirements for vehicles and systems. A public accommodation subject to...

  18. Cyclosporine A inhibits colorectal cancer proliferation probably by regulating expression levels of c-Myc, p21(WAF1/CIP1) and proliferating cell nuclear antigen.

    PubMed

    Masuo, Takashige; Okamura, Shinichi; Zhang, Yajing; Mori, Masatomo

    2009-11-18

    The present study investigated the role of calcineurin (CaN) in the proliferation of human colorectal cancers. CaN activity and protein expression were increased in human colorectal cancers. Nuclear transcription factor NFAT, a physiological substrate for CaN, was activated in human colon cancer specimen as well as in the human colon cancer cell lines. CaN inhibitor cyclosporine A (CsA) reduced cell growth in these cell lines. CsA decreased the expressions of c-Myc and the proliferating cell nuclear antigen (PCNA) but also increased p21(WAF1/CIP1) expression. Our results suggest that CaN promotes colorectal cancer proliferation probably by regulating levels of c-Myc, p21(WAF1/CIP1), and PCNA.

  19. Crystalline lens MTF measurement during simulated accommodation

    NASA Astrophysics Data System (ADS)

    Borja, David; Takeuchi, Gaku; Ziebarth, Noel; Acosta, Ana C.; Manns, Fabrice; Parel, Jean-Marie

    2005-04-01

    Purpose: To design and test an optical system to measure the optical quality of post mortem lenses during simulated accommodation. Methods: An optical bench top system was designed to measure the point spread function and calculate the modulation transfer function (MTF) of monkey and human ex-vivo crystalline lenses. The system consists of a super luminescent diode emitting at 850nm, collimated into a 3mm beam which is focused by the ex-vivo lens under test. The intensity distribution at the focus (point spread function) is re-imaged and magnified onto a beam profiler CCD camera. The optical quality in terms of spatial frequency response (modulation transfer function) is calculated by Fourier transform of the point spread function. The system was used on ex-vivo lenses with attached zonules, ciliary body and sclera. The sclera was glued to 8 separate PMMA segments and stretched radial by 5mm on an accommodation simulating lens stretching device. The point spread function was measured for each lens in the relaxed and stretched state for 5 human (ages 38-86 years) and 5 cynomolgus monkey (ages 53 - 67 months) fresh post mortem crystalline lenses. Results: Stretching induced measurable changes in the MTF. The cutoff frequency increased from 54.4+/-13.6 lp/mm unstretched to 59.5+/-21.4 lp/mm stretched in the post-presbyopic human and from 51.9+/-24.7 lp/mm unstretched to 57.7+/-18.5 lp/mm stretched cynomolgus monkey lenses. Conclusion: The results demonstrate the feasibility of measuring the optical quality of ex-vivo human and cynomolgus monkey lenses during simulated accommodation. Additional experiments are underway to quantify changes in optical quality induced by stretching.

  20. Skylab Workshop experience in experiment accommodation

    NASA Technical Reports Server (NTRS)

    Hanlon, W. H.; Hassel, R. R.

    1974-01-01

    This paper examines the experiment support facilities available from the Orbital Workshop (OWS) module of the Skylab. Experiments and associated support provisions have been selected and described to illustrate the various accommodations and degree of complexities involved in the integration of these experiments into the Workshop. The interfaces described start with the simple and proceed to the complex. On the basis of the experience gained in integrating the experiments into the Workshop, conclusions are drawn and suggestions are made on ways to facilitate future experiment operations and at the same time simplify and reduce the cost of integration efforts.

  1. Strain accommodation in inelastic deformation of glasses

    SciTech Connect

    Murali, P.; Ramamurty, U.; Shenoy, Vijay B.

    2007-01-01

    Motivated by recent experiments on metallic glasses, we examine the micromechanisms of strain accommodation including crystallization and void formation during inelastic deformation of glasses by employing molecular statics simulations. Our atomistic simulations with Lennard-Jones-like potentials suggests that a softer short range interaction between atoms favors crystallization. Compressive hydrostatic strain in the presence of a shear strain promotes crystallization whereas a tensile hydrostatic strain is found to induce voids. The deformation subsequent to the onset of crystallization includes partial reamorphization and recrystallization, suggesting important atomistic mechanisms of plastic dissipation in glasses.

  2. Measurement of Gas-Surface Accommodation

    NASA Astrophysics Data System (ADS)

    Trott, W. M.; Rader, D. J.; Castañeda, J. N.; Torczynski, J. R.; Gallis, M. A.

    2008-12-01

    Thermal accommodation coefficients have been determined for a variety of gas-surface combinations using an experimental apparatus developed to measure both the pressure dependence of the conductive heat flux and the variation of gas density between parallel plates separated by a gas-filled gap. Effects of gas composition, surface roughness and surface contamination have been examined with this system, and the behavior of gas mixtures has also been explored. Results are discussed in comparison with previous parallel-plate experimental studies as well as numerical simulations.

  3. Evolution of a polyphenism by genetic accommodation.

    PubMed

    Suzuki, Yuichiro; Nijhout, H Frederik

    2006-02-03

    Polyphenisms are adaptations in which a genome is associated with discrete alternative phenotypes in different environments. Little is known about the mechanism by which polyphenisms originate. We show that a mutation in the juvenile hormone-regulatory pathway in Manduca sexta enables heat stress to reveal a hidden reaction norm of larval coloration. Selection for increased color change in response to heat stress resulted in the evolution of a larval color polyphenism and a corresponding change in hormonal titers through genetic accommodation. Evidently, mechanisms that regulate developmental hormones can mask genetic variation and act as evolutionary capacitors, facilitating the origin of novel adaptive phenotypes.

  4. Assessment of launch site accommodations versus Spacelab payload requirements

    NASA Technical Reports Server (NTRS)

    1977-01-01

    The Kennedy launch site capability for accommodating spacelab payload operations was assessed. Anomalies between facility accommodations and requirements for the Spacelab III (Strawman), OA Mission 83-2, Dedicated Life Sciences, and Combined Astronomy missions are noted. Recommendations for revision of the accommodations handbook are summarized.

  5. 46 CFR 154.325 - Accommodation, service, and control spaces.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 5 2013-10-01 2013-10-01 false Accommodation, service, and control spaces. 154.325... Equipment Ship Arrangements § 154.325 Accommodation, service, and control spaces. (a) Accommodation, service, and control spaces must be outside the cargo area. (b) If a hold space having a cargo...

  6. 46 CFR 108.211 - Miscellaneous accommodation spaces.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Miscellaneous accommodation spaces. 108.211 Section 108... DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.211 Miscellaneous accommodation spaces. (a) Each unit must have enough facilities for personnel to wash their own...

  7. 46 CFR 108.197 - Construction of accommodation spaces.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Construction of accommodation spaces. 108.197 Section... UNITS DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.197 Construction of accommodation spaces. (a) Each sleeping, mess, recreational, or hospital space that is adjacent to...

  8. 46 CFR 108.211 - Miscellaneous accommodation spaces.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Miscellaneous accommodation spaces. 108.211 Section 108... DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.211 Miscellaneous accommodation spaces. (a) Each unit must have enough facilities for personnel to wash their own...

  9. 46 CFR 108.197 - Construction of accommodation spaces.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Construction of accommodation spaces. 108.197 Section... UNITS DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.197 Construction of accommodation spaces. (a) Each sleeping, mess, recreational, or hospital space that is adjacent to...

  10. 46 CFR 108.197 - Construction of accommodation spaces.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Construction of accommodation spaces. 108.197 Section... UNITS DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.197 Construction of accommodation spaces. (a) Each sleeping, mess, recreational, or hospital space that is adjacent to...

  11. 46 CFR 108.211 - Miscellaneous accommodation spaces.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Miscellaneous accommodation spaces. 108.211 Section 108... DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.211 Miscellaneous accommodation spaces. (a) Each unit must have enough facilities for personnel to wash their own...

  12. 28 CFR 36.310 - Transportation provided by public accommodations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 1 2013-07-01 2013-07-01 false Transportation provided by public... BASIS OF DISABILITY BY PUBLIC ACCOMMODATIONS AND IN COMMERCIAL FACILITIES Specific Requirements § 36.310 Transportation provided by public accommodations. (a) General. (1) A public accommodation that...

  13. Quick and Easy Adaptations and Accommodations for Early Childhood Students

    ERIC Educational Resources Information Center

    Breitfelder, Leisa M.

    2008-01-01

    Research-based information is used to support the idea of the use of adaptations and accommodations for early childhood students who have varying disabilities. Multiple adaptations and accommodations are outlined. A step-by-step plan is provided on how to make specific adaptations and accommodations to fit the specific needs of early childhood…

  14. An Analysis and Rejection of Arguments for Religious Accommodation

    ERIC Educational Resources Information Center

    Kline, Lisa Anne

    2009-01-01

    This dissertation provides a comprehensive critical analysis of six main arguments for religious accommodation, with a specific focus on fundamentalist religious groups and the accommodation of their practices within liberal democratic societies. This analysis reveals that the types of practices that these arguments aim to accommodate primarily…

  15. 46 CFR 154.325 - Accommodation, service, and control spaces.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 5 2011-10-01 2011-10-01 false Accommodation, service, and control spaces. 154.325... Equipment Ship Arrangements § 154.325 Accommodation, service, and control spaces. (a) Accommodation, service, and control spaces must be outside the cargo area. (b) If a hold space having a cargo...

  16. 46 CFR 108.197 - Construction of accommodation spaces.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Construction of accommodation spaces. 108.197 Section... UNITS DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.197 Construction of accommodation spaces. (a) Each sleeping, mess, recreational, or hospital space that is adjacent to...

  17. 46 CFR 108.211 - Miscellaneous accommodation spaces.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Miscellaneous accommodation spaces. 108.211 Section 108... DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.211 Miscellaneous accommodation spaces. (a) Each unit must have enough facilities for personnel to wash their own...

  18. 46 CFR 154.325 - Accommodation, service, and control spaces.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Accommodation, service, and control spaces. 154.325... Equipment Ship Arrangements § 154.325 Accommodation, service, and control spaces. (a) Accommodation, service, and control spaces must be outside the cargo area. (b) If a hold space having a cargo...

  19. 46 CFR 108.211 - Miscellaneous accommodation spaces.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Miscellaneous accommodation spaces. 108.211 Section 108... DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.211 Miscellaneous accommodation spaces. (a) Each unit must have enough facilities for personnel to wash their own...

  20. 46 CFR 108.197 - Construction of accommodation spaces.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Construction of accommodation spaces. 108.197 Section... UNITS DESIGN AND EQUIPMENT Construction and Arrangement Accommodation Spaces § 108.197 Construction of accommodation spaces. (a) Each sleeping, mess, recreational, or hospital space that is adjacent to...

  1. hERG1/Kv11.1 activation stimulates transcription of p21waf/cip in breast cancer cells via a calcineurin-dependent mechanism

    PubMed Central

    Perez-Neut, Mathew; Rao, Vidhya R.; Gentile, Saverio

    2016-01-01

    The function of Kv11.1 is emerging in breast cancer biology, as a growing body of evidence indicates that the hERG1/Kv11.1 potassium channel is aberrantly expressed in several cancer types including breast cancers. The biological effects of Kv11.1 channel blockers and their associated side effects are very well known but the potential use of Kv11.1 activators as an anticancer strategy are still unexplored. In our previous work, we have established that stimulation of the Kv11.1 potassium channel activates a senescent-like program that is characterized by a significant increase in tumor suppressor protein levels, such as p21waf/cip and p16INK4A. In this study we investigated the mechanism linking Kv11.1 stimulation to augmentation of p21waf/cip protein level. We have demonstrated that the Kv11.1 channel activator NS1643 activates a calcineurin-dependent transcription of p21waf/cip and that this event is fundamental for the inhibitory effect of NS1643 on cell proliferation. Our results reveal a novel mechanism by which stimulation of Kv11.1 channel leads to transcription of a potent tumor suppressor and suggest a potential therapeutic use for Kv11.1 channel activators. PMID:25945833

  2. IL-12-dependent innate immunity arrests endothelial cells in G0-G1 phase by a p21(Cip1/Waf1)-mediated mechanism.

    PubMed

    Napione, Lucia; Strasly, Marina; Meda, Claudia; Mitola, Stefania; Alvaro, Maria; Doronzo, Gabriella; Marchiò, Serena; Giraudo, Enrico; Primo, Luca; Arese, Marco; Bussolino, Federico

    2012-12-01

    Innate immunity may activate paracrine circuits able to entail vascular system in the onset and progression of several chronic degenerative diseases. In particular, interleukin (IL)-12 triggers a genetic program in lymphomononuclear cells characterized by the production of interferon-γ and specific chemokines resulting in an angiostatic activity. The aim of this study is to identify molecules involved in the regulation of cell cycle in endothelial cells co-cultured with IL-12-stimulated lymphomonuclear cells. By using a transwell mediated co-culture system we demonstrated that IL-12-stimulated lymphomonuclear cells induce an arrest of endothelial cells cycle in G1, which is mainly mediated by the up-regulation of p21(Cip1/Waf1), an inhibitor of cyclin kinases. This effect requires the activation of STAT1, PKCδ and p38 MAPK, while p53 is ineffective. In accordance, siRNA-dependent silencing of these molecules in endothelial cells inhibited the increase of p21(Cip1/Waf1) and the modification in cell cycle promoted by IL-12-stimulated lymphomonuclear cells. These results indicate that the angiostatic action of IL-12-stimulated lymphomononuclear cells may lie in the capability to arrest endothelial cells in G1 phase through a mechanisms mainly based on the specific up-regulation of p21(Cip1/Waf1) induced by the combined activity of STAT1, PKCδ and p38 MAPK.

  3. Comparative effects of biological and chemical dispersants on the bioavailability and toxicity of crude oil to early life stages of marine medaka (Oryzias melastigma).

    PubMed

    Mu, Jingli; Jin, Fei; Ma, Xindong; Lin, Zhongsheng; Wang, Juying

    2014-11-01

    The authors assessed the bioavailability and chronic toxicity of water-accommodated fractions of crude oil (WAFs) and 2 dispersants plus dispersed crude oil (chemical dispersant + crude oil [CE-WAF] and biological dispersant + crude oil [BE-WAF]) on the early life stages of marine medaka, Oryzias melastigma. The results showed that the addition of the 2 dispersants caused a 3- and 4-fold increase in concentrations of summed priority polycyclic aromatic hydrocarbons (PAHs) and high-molecular-weight PAHs with 3 or more benzene rings. The chemical and biological dispersants increased the bioavailability (as measured by ethoxyresorufin-O-dethylase activity) of crude oil 6-fold and 3-fold, respectively. Based on nominal concentrations, chronic toxicity (as measured by deformity) in WAFs exhibited a 10-fold increase in CE-WAF and a 3-fold increase in BE-WAF, respectively. When total petroleum hydrocarbon was measured, the differences between WAF and CE-WAF treatments disappeared, and CE-WAF was approximately 10 times more toxic than BE-WAF. Compared with the chemical dispersant, the biological dispersant possibly modified the toxicity of oil hydrocarbons because of the increase in the proportion of 2- and 3-ringed PAHs in water. The chemical and biological dispersants enhanced short-term bioaccumulation and toxicity, through different mechanisms. These properties should be considered in addition to their efficacy in degrading oil when oil spill management strategies are selected.

  4. [Dissociated near reflex and accommodative convergence excess].

    PubMed

    Gräf, M; Becker, R; Kloss, S

    2004-10-01

    We report on an 8-year-old boy whose near reflex could be elicited exclusively when the left eye was fixing (LF) but not when the right eye was fixing (RF). With RE +1.25/-1.25/169 degrees and LE +1.0/-0.75/24 degrees, the visual acuity was 1.0 OU at 5 m and RE 0.5, LE 1.0 at 0.3 m improving to 1.0 OU by a near addition of 3.0 D. Stereopsis was 100 degrees (Titmus test). The prism and cover test revealed an esophoria of 4 degrees at 5 m. At 3 m there was an esophoria of 6 degrees (RF) and an esotropia of 28 degrees (LF), compensating to an esophoria of 3 degrees (RF/LF) with a near addition of 3.0 D. Accommodation and the pupillary near reaction (OU) were hardly elicitable during RF. During LF, retinoscopy revealed an accommodation of 8 D (OU) and the pupils constricted normally. Correction by bifocal glasses yielded orthotropia with random dot stereopsis at near.

  5. The OEOP Duties of Reasonable Accommodation

    NASA Technical Reports Server (NTRS)

    Coppedge, Angela

    1995-01-01

    I was fortunate enough to be assigned two assignments during my ten weeks here at NASA's Langley Research Center, in the Office of Equal Opportunity Programs (OEOP). One of my projects gave me the chance to gain experience in developing calculation formulas for the EXCEL computer system, while my second project gave me the chance to put my research skills and legal knowledge to use. The function of the OEOP is to ensure the adherence to personnel policy and practices in the employment, development, advancement and treatment of Federal employees and applicants for employment. This includes veterans and disabled as well. My initial project involved the research of hiring and promotion among the different minorities and females employed here at Langley. The objective of my first project was to develop graphs that showed the number of promotions during the past five years for each minority group here on the Center. I also had to show the average number of years it took for each promotion. The objective of my second and main research project was to find and research cases regarding the reasonable accommodation of disabled workers. The research of these cases is to ensure that individuals with disabilities are provided the necessary accommodations that are essential to the function of their job.

  6. Manned Mars mission accommodation: Sprint mission

    NASA Astrophysics Data System (ADS)

    Cirillo, William M.; Kaszubowski, Martin J.; Ayers, J. Kirk; Llewellyn, Charles P.; Weidman, Deene J.; Meredith, Barry D.

    1988-04-01

    The results of a study conducted at the NASA-LaRC to assess the impacts on the Phase 2 Space Station of Accommodating a Manned Mission to Mars are documented. In addition, several candidate transportation node configurations are presented to accommodate the assembly and verification of the Mars Mission vehicles. This study includes an identification of a life science research program that would need to be completed, on-orbit, prior to mission departure and an assessment of the necessary orbital technology development and demonstration program needed to accomplish the mission. Also included is an analysis of the configuration mass properties and a preliminary analysis of the Space Station control system sizing that would be required to control the station. Results of the study indicate the Phase 2 Space Station can support a manned mission to Mars with the addition of a supporting infrastructure that includes a propellant depot, assembly hanger, and a heavy lift launch vehicle to support the large launch requirements.

  7. Manned Mars mission accommodation: Sprint mission

    NASA Technical Reports Server (NTRS)

    Cirillo, William M.; Kaszubowski, Martin J.; Ayers, J. Kirk; Llewellyn, Charles P.; Weidman, Deene J.; Meredith, Barry D.

    1988-01-01

    The results of a study conducted at the NASA-LaRC to assess the impacts on the Phase 2 Space Station of Accommodating a Manned Mission to Mars are documented. In addition, several candidate transportation node configurations are presented to accommodate the assembly and verification of the Mars Mission vehicles. This study includes an identification of a life science research program that would need to be completed, on-orbit, prior to mission departure and an assessment of the necessary orbital technology development and demonstration program needed to accomplish the mission. Also included is an analysis of the configuration mass properties and a preliminary analysis of the Space Station control system sizing that would be required to control the station. Results of the study indicate the Phase 2 Space Station can support a manned mission to Mars with the addition of a supporting infrastructure that includes a propellant depot, assembly hangar, and a heavy lift launch vehicle to support the large launch requirements.

  8. Accommodative lag and fluctuations when optical aberrations are manipulated.

    PubMed

    Gambra, Enrique; Sawides, Lucie; Dorronsoro, Carlos; Marcos, Susana

    2009-06-09

    We evaluated the accommodative response to a stimulus moving from 0 to 6 D following a staircase function under natural, corrected, and induced optical aberrations, using an adaptive-optics (AO) electromagnetic deformable mirror. The accommodative response of the eye (through the mirror) and the change of aberrations were measured on 5 subjects using a Hartmann-Shack wavefront sensor operating at 12.8 Hz. Five conditions were tested: (1) natural aberrations, (2) AO correction of the unaccommodated state and induction (over 6-mm pupils) of (3) +1 microm and (4) -1 microm of spherical aberration and (5) -2 microm of vertical coma. Four subjects showed a better accommodative response with AO correction than with their natural aberrations. The induction of negative spherical aberration also produced a better accommodative response in the same subjects. Accommodative lag increased in all subjects when positive spherical aberration and coma were induced. Fluctuations of the accommodative response (computed during each 1-D period of steady accommodation) increased with accommodative response when high-order aberrations were induced. The largest fluctuations occurred for induced negative spherical aberration and the smallest for natural and corrected aberrations. The study demonstrates that aberrations influence accommodative lag and fluctuations of accommodation and that correcting aberrations improves rather than compromises the accommodative response.

  9. ADAPT: Attention Deficit Accommodation Plan for Teaching. Teacher Accommodation Planbook [and] Student Planbook.

    ERIC Educational Resources Information Center

    Parker, Harvey C.

    This student workbook and accompanying teacher's guide are designed to help children with attention deficit disorders (ADD). The Attention Deficit Accommodation Plan for Teaching (ADAPT) teacher planbook guides the teacher in evaluating the students' areas of difficulty. This evaluation is the basis for the design and implementation of classroom…

  10. Accommodating Your Data: The Use and Misuse of Accommodation Theory in Sociolinguistics.

    ERIC Educational Resources Information Center

    Meyerhoff, Miriam

    1998-01-01

    Argues for a more rigorous application of accommodation theory in sociolinguistics, presenting an example of how such rigor might be pursued in an analysis of conversational Bislama, a creole spoken in the Republic of Vanuatu. Focus is on the link between speakers' identities and their linguistic behavior. (MSE)

  11. Accommodation Hell, or, To Hell with Accommodation: The ADA and the Administration.

    ERIC Educational Resources Information Center

    Robinson, William L.

    This material is designed to help faculty understand the requirements of the Americans with Disabilities Act of 1990 (ADA). A brief overview notes three key considerations: the definition of disability, reasonable accommodation, and undue hardship, and then discusses faculty liability and responsibility for discriminatory acts. The balance of the…

  12. Understanding Multiplication of Fractions.

    ERIC Educational Resources Information Center

    Sweetland, Robert D.

    1984-01-01

    Discussed the use of Cuisenaire rods in teaching the multiplication of fractions. Considers whole number times proper fraction, proper fraction multiplied by proper fraction, mixed number times proper fraction, and mixed fraction multiplied by mixed fractions. (JN)

  13. BRG1 controls the activity of the retinoblastoma protein via regulation of p21CIP1/WAF1/SDI.

    PubMed

    Kang, Hyeog; Cui, Kairong; Zhao, Keji

    2004-02-01

    The ubiquitous mammalian chromatin-remodeling SWI/SNF-like BAF complexes play critical roles in tumorigenesis. It was suggested that the direct interaction of BRG1 with the retinoblastoma protein pRB is required for regulation of cell cycle progression by pRB. We present evidence that the BRG1-containing complexes regulate the expression of the cdk inhibitor p21(CIP1/WAF1/SDI). Furthermore, we show that the physical interaction between BRG1 and pRB is not required for induction of cell growth arrest and transcriptional repression of E2F target genes by pRB. Instead, BRG1 activates pRB by inducing its hypophosphorylation through up-regulation of the cdk inhibitor p21. The hypophosphorylation of pRB is reinforced by down-regulation of critical components, including cdk2, cyclin E, and cyclin D, in the pRB regulatory network. We demonstrate that up-regulation of p21 by BRG1 is necessary to induce formation of flat cells, growth arrest, and finally, cell senescence. Our results suggest that the BRG1-containing complexes control cellular proliferation and senescence by modulating the pRB pathway via multiple mechanisms.

  14. Cigarette smoke triggers code red: p21CIP1/WAF1/SDI1 switches on danger responses in the lung.

    PubMed

    Tuder, Rubin M; Yun, Jeong H; Graham, Brian B

    2008-07-01

    The article by Yao and coworkers in this issue (Am. J. Respir. Cell Mol. Biol. 2008;39:7-18) reveals that the cyclin-dependent kinase inhibitor p21CIP1/WAF1/SDI1 (designated hereafter as p21), which has been linked to cell cycle growth arrest due to stress or danger cell responses, may modulate alveolar inflammation and alveolar destruction, and thus enlightens our present understanding of how the lung senses injury due to cigarette smoke and integrates these responses with those that activate inflammatory pathways potentially harmful to the lung. Furthermore, the interplay of p21 and cellular processes involving cell senescence and the imbalance of cell proliferation/apoptosis may provide us with a more logical explanation of how p21, acting as a sensor of cellular stress, might have such potent and wide roles in lung responses triggered by cigarette smoke. Molecular switches, ontologically designed for the protection of the host, are now hijacked by injurious stresses (such as cigarette smoke), leading to organ damage.

  15. Adaptive Accommodation Control Method for Complex Assembly

    NASA Astrophysics Data System (ADS)

    Kang, Sungchul; Kim, Munsang; Park, Shinsuk

    Robotic systems have been used to automate assembly tasks in manufacturing and in teleoperation. Conventional robotic systems, however, have been ineffective in controlling contact force in multiple contact states of complex assemblythat involves interactions between complex-shaped parts. Unlike robots, humans excel at complex assembly tasks by utilizing their intrinsic impedance, forces and torque sensation, and tactile contact clues. By examining the human behavior in assembling complex parts, this study proposes a novel geometry-independent control method for robotic assembly using adaptive accommodation (or damping) algorithm. Two important conditions for complex assembly, target approachability and bounded contact force, can be met by the proposed control scheme. It generates target approachable motion that leads the object to move closer to a desired target position, while contact force is kept under a predetermined value. Experimental results from complex assembly tests have confirmed the feasibility and applicability of the proposed method.

  16. Astrophysical payload accommodation on the space station

    NASA Technical Reports Server (NTRS)

    Woods, B. P.

    1985-01-01

    Surveys of potential space station astrophysics payload requirements and existing point mount design concepts were performed to identify potential design approaches for accommodating astrophysics instruments from space station. Most existing instrument pointing systems were designed for operation from the space shuttle and it is unlikely that they will sustain their performance requirements when exposed to the space station disturbance environment. The technology exists or is becoming available so that precision pointing can be provided from the space station manned core. Development of a disturbance insensitive pointing mount is the key to providing a generic system for space station. It is recommended that the MSFC Suspended Experiment Mount concept be investigated for use as part of a generic pointing mount for space station. Availability of a shirtsleeve module for instrument change out, maintenance and repair is desirable from the user's point of view. Addition of a shirtsleeve module on space station would require a major program commitment.

  17. FDI and Accommodation Using NN Based Techniques

    NASA Astrophysics Data System (ADS)

    Garcia, Ramon Ferreiro; de Miguel Catoira, Alberto; Sanz, Beatriz Ferreiro

    Massive application of dynamic backpropagation neural networks is used on closed loop control FDI (fault detection and isolation) tasks. The process dynamics is mapped by means of a trained backpropagation NN to be applied on residual generation. Process supervision is then applied to discriminate faults on process sensors, and process plant parameters. A rule based expert system is used to implement the decision making task and the corresponding solution in terms of faults accommodation and/or reconfiguration. Results show an efficient and robust FDI system which could be used as the core of an SCADA or alternatively as a complement supervision tool operating in parallel with the SCADA when applied on a heat exchanger.

  18. The acute toxicity of chemically and physically dispersed crude oil to key Arctic species under Arctic conditions during the open water season.

    PubMed

    Gardiner, William W; Word, Jack Q; Word, Jack D; Perkins, Robert A; McFarlin, Kelly M; Hester, Brian W; Word, Lucinda S; Ray, Collin M

    2013-10-01

    The acute toxicity of physically and chemically dispersed crude oil and the dispersant Corexit 9500 were evaluated for key Arctic species. The copepod Calanus glacialis, juvenile Arctic cod (Boreogadus saida), and larval sculpin (Myoxocephalus sp.) were tested under conditions representative of the Beaufort and Chukchi Seas during the ice-free season. The toxicity of 3 water-accommodated fractions (WAF) of Alaska North Slope crude oil was examined with spiked, declining exposures. A dispersant-only test was conducted with the copepod C. glacialis. Each preparation with oil (WAF, breaking wave WAF [BWWAF], and chemically enhanced WAF [CEWAF]) produced distinct suites of hydrocarbon constituents; the total concentrations of oil were lowest in WAF and highest in CEWAF preparations. The relative sensitivity for the different species and age classes was similar within each WAF type. Median lethal concentration values based on total petroleum hydrocarbons ranged from 1.6 mg/L to 4.0 mg/L for WAF and BWWAF treatments and from 22 mg/L to 62 mg/L for CEWAF. For Corexit 9500 exposures, median lethal concentration values ranged from 17 mg/L to 50 mg/L. The differences in the relative toxicity among the accommodated fractions indicated that the majority of petroleum hydrocarbons in the CEWAF are in less acutely toxic forms than the components that dominate the WAF or BWWAF. Further evaluation showed that the parent polycyclic aromatic hydrocarbon compounds, specifically naphthalene, were highly correlated to acute toxicity.

  19. The effects of Qi-Qong ocular exercise on accommodation.

    PubMed

    Shih, Y F; Lin, L L; Hwang, C Y; Huang, J K; Hung, P T; Hou, P K

    1995-01-01

    Visual-training methods in management of myopia are suggested as possibly leading to normal vision or an improvement in the refractive state. The purpose of this work was to investigate the hypothesis that a Qi-Qong ocular exercise improves visual function by training the accommodation bias. Variations of pupil size, accommodative amplitude, latency and speed of accommodative response and accommodative adaptation were evaluated objectively. The accommodative state was monitored with an objective infrared refractometer (Nidek AA-2000). Subjects were divided into two groups: experimental (n = 9), and control (n = 8). Subjects of the experimental group undertook the Qi-Qong ocular exercise for at least three years, and were able to perform the exercise smoothly and gently. Subjects of the control group had no knowledge of the Qi-Qong ocular exercise, but were given the same testing procedures as the experimental group. Results showed that Qi-Qong ocular exercise can improve the accommodative amplitude and accelerate the accommodative response slightly, but there was no effect on the latency of accommodative response. Furthermore, the level of accommodative adaptation was elevated, and the pupil became slight miotic. Therefore, the mechanism of visual improvement may undergo a great accommodative adaptation and produce a pinhole effect by miosis of the pupil. Methods of visual training can produce a false image of visual improvement from an enhanced parasympathetic response to a task, but this effect may be a factor that induces progression of myopia.

  20. Mystery Fractions

    ERIC Educational Resources Information Center

    Bhattacharyya, Sonalee; Namakshi, Nama; Zunker, Christina; Warshauer, Hiroko K.; Warshauer, Max

    2016-01-01

    Making math more engaging for students is a challenge that every teacher faces on a daily basis. These authors write that they are constantly searching for rich problem-solving tasks that cover the necessary content, develop critical-thinking skills, and engage student interest. The Mystery Fraction activity provided here focuses on a key number…

  1. Pitch Fractionation.

    DTIC Science & Technology

    1981-12-15

    13 3. Solvent Fractionation Experiments .................................... 15 4. Fourier Transform Infrared Spectra for A240 Petrolem Pitch AG 12...34 and Mesophase Pitch AG 164B ............................... 21 5. Fourier Transform Infrared Spectra ................................... 23 6...compared by Fourier transform infrared (FTIR) analysis using a Digilab Model FTS 14 spectrophotometer (Rockwell International, Anaheim, California

  2. Immunohistochemical markers of cell cycle control applied to ovarian and primary peritoneal surface epithelial neoplasms: p21(WAF1/CIP1) predicts survival and good response to platinin-based chemotherapy.

    PubMed

    Costa, M J; Hansen, C L; Walls, J E; Scudder, S A

    1999-06-01

    Immunohistochemistry for p53, p21(WAF1/CIP1), and Ki-67 provides insight into the molecular events controlling the cell cycle. We tested the hypothesis that these cell cycle markers will aid in the clinical evaluation of ovarian and primary peritoneal surface epithelial neoplasms (SENs). Paraffin sections from a retrospective surgical series of 117 SENs were immunostained with anti-p53 (clone DO7, Novacastra Laboratories, UK), anti-p21(WAF1/CIP1) (clone EA10, Oncogene Science, Cambridge, MA), and anti-Ki-67 (clone MIB-1, Immunotech, Westbrook, ME). The Ki-67 proliferation index (Ki-67PI) and immunoreactivity were evaluated. One hundred seventeen SENs reacted as follows: p53 50%+ and p21(WAF1/CIP1) 65%+. Ki-67PI ranged from 4% to 88% (mean/median = 44/46%). p53 reactivity associated with transitional cell histology, decreased p21(WAF1/CIP1) staining, increased Ki-67PI, architectural/nuclear grade, and stage (P < .05, 1 x 10(-7), .01, .05/.0001, .001,). p21(WAF1/CIP1) staining was associated with endometrioid/clear cell histology, decreased Ki-67PI, architectural/nuclear grade, and stage (P < 05/.05, .05, .01/1 x 10(-8), 1 x 10(-5)). Ki-67PI associated with increased architectural/nuclear grade but not mucinous histology (P < 1 x 10(-5)/1 x 10(-6), .01). Sixty-seven patients had disease at last follow-up; 53 were dead of disease at 0 to 67 months (mean/median, 21/18), and 14 were alive with disease at 12 to 224 months (mean/median, 56/40). Fifty patients were disease free at 5 to 214 months (mean/median, 59/41). Predictors of survival include decreased Ki-67PI, stage, architectural/nuclear grade (P < 1 x 10(-6), 1 x 10(-10), 1 x 10(-10)/.005) and p21(WAF1/CIP1) IMS (multivariate P < 1 x 10(-6)). p21(WAF1/CIP1), a potent inhibitor of cyclin-dependent kinases necessary for cell cycle progression, functions as a key checkpoint in cell cycle control. Immunoreactivity for p21(WAF1/CIP1) provides prognostic information independent of other histological and clinical

  3. Regional Sign Language Varieties in Contact: Investigating Patterns of Accommodation.

    PubMed

    Stamp, Rose; Schembri, Adam; Evans, Bronwen G; Cormier, Kearsy

    2016-01-01

    Short-term linguistic accommodation has been observed in a number of spoken language studies. The first of its kind in sign language research, this study aims to investigate the effects of regional varieties in contact and lexical accommodation in British Sign Language (BSL). Twenty-five participants were recruited from Belfast, Glasgow, Manchester, and Newcastle and paired with the same conversational partner. Participants completed a "spot-the-difference" task which elicited a considerable amount of contrasting regionally specific sign data in the participant-confederate dyads. Accommodation was observed during the task with younger signers accommodating more than older signers. The results are interpreted with reference to the relationship between language contact and lexical accommodation in BSL, and address how further studies could help us better understand how contact and accommodation contribute to language change more generally.

  4. Accommodation in Astigmatic Children During Visual Task Performance

    PubMed Central

    Harvey, Erin M.; Miller, Joseph M.; Apple, Howard P.; Parashar, Pavan; Twelker, J. Daniel; Crescioni, Mabel; Davis, Amy L.; Leonard-Green, Tina K.; Campus, Irene; Sherrill, Duane L.

    2014-01-01

    Purpose. To determine the accuracy and stability of accommodation in uncorrected children during visual task performance. Methods. Subjects were second- to seventh-grade children from a highly astigmatic population. Measurements of noncycloplegic right eye spherical equivalent (Mnc) were obtained while uncorrected subjects performed three visual tasks at near (40 cm) and distance (2 m). Tasks included reading sentences with stimulus letter size near acuity threshold and an age-appropriate letter size (high task demands) and viewing a video (low task demand). Repeated measures ANOVA assessed the influence of astigmatism, task demand, and accommodative demand on accuracy (mean Mnc) and variability (mean SD of Mnc) of accommodation. Results. For near and distance analyses, respectively, sample size was 321 and 247, mean age was 10.37 (SD 1.77) and 10.30 (SD 1.74) years, mean cycloplegic M was 0.48 (SD 1.10) and 0.79 diopters (D) (SD 1.00), and mean astigmatism was 0.99 (SD 1.15) and 0.75 D (SD 0.96). Poor accommodative accuracy was associated with high astigmatism, low task demand (video viewing), and high accommodative demand. The negative effect of accommodative demand on accuracy increased with increasing astigmatism, with the poorest accommodative accuracy observed in high astigmats (≥3.00 D) with high accommodative demand/high hyperopia (1.53 D and 2.05 D of underaccommodation for near and distant stimuli, respectively). Accommodative variability was greatest in high astigmats and was uniformly high across task condition. No/low and moderate astigmats showed higher variability for the video task than the reading tasks. Conclusions. Accuracy of accommodation is reduced in uncorrected children with high astigmatism and high accommodative demand/high hyperopia, but improves with increased visual task demand (reading). High astigmats showed the greatest variability in accommodation. PMID:25103265

  5. Fraction Reduction through Continued Fractions

    ERIC Educational Resources Information Center

    Carley, Holly

    2011-01-01

    This article presents a method of reducing fractions without factoring. The ideas presented may be useful as a project for motivated students in an undergraduate number theory course. The discussion is related to the Euclidean Algorithm and its variations may lead to projects or early examples involving efficiency of an algorithm.

  6. Research centrifuge accommodations on Space Station Freedom

    NASA Technical Reports Server (NTRS)

    Arno, Roger D.; Horkachuk, Michael J.

    1990-01-01

    Life sciences research using plants and animals on the Space Station Freedom requires the ability to maintain live subjects in a safe and low stress environment for long durations at microgravity and at one g. The need for a centrifuge to achieve these accelerations is evident. Programmatic, technical, and cost considerations currently favor a 2.5 meter diameter centrifuge located either in the end cone of a Space Station Freedom node or in a separate module. A centrifuge facility could support a mix of rodent, plant, and small primate habitats. An automated cage extractor could be used to remove modular habitats in pairs without stopping the main rotor, minimizing the disruption to experiment protocols. The accommodation of such a centrifuge facility on the Space Station represents a significant demand on the crew time, power, data, volume, and logistics capability. It will contribute to a better understanding of the effects of space flight on humans, an understanding of plant growth in space for the eventual production of food, and an understanding of the role of gravity in biological processes.

  7. Accommodating Actuator Failures in Flight Control Systems

    NASA Technical Reports Server (NTRS)

    Hess, R. A.; Siwakosit, W.; Chung, J.

    1998-01-01

    A technique for the design of flight control systems that can accommodate a set of actuator failures is presented. As employed herein, an actuator failure is defined as any change in the parametric model of the actuator which can adversely affect actuator performance. The technique is based upon the formulation of a fixed feedback topology which ensures at least stability in the presence of the failures in the set. The fixed compensation is obtained from a loop-shaping design procedure similar to Quantitative Feedback Theory and provides stability robustness in the presence of uncertainty in the vehicle dynamics caused by the failures. System adaptation to improve performance after actuator failure(s) occurs through a static gain adjustment in the compensator followed by modification of the system prefilter. Precise identification of the vehicle dynamics is unnecessary. Application to a single-input, single-output design using a simplified model of the longitudinal dynamics of the NASA High Angle of Attack Research Vehicle is discussed. Non-real time simulations of the system including a model of the pilot demonstrate the effectiveness and limitations of the approach.

  8. p21{sup WAF1/CIP1} deficiency induces mitochondrial dysfunction in HCT116 colon cancer cells

    SciTech Connect

    Kim, Ae Jeong; Jee, Hye Jin; Song, Naree; Kim, Minjee; Jeong, Seon-Young; Yun, Jeanho

    2013-01-11

    Highlights: Black-Right-Pointing-Pointer p21{sup -/-} HCT116 cells exhibited an increase in mitochondrial mass. Black-Right-Pointing-Pointer The expression levels of PGC-1{alpha} and AMPK were upregulated in p21{sup -/-} HCT116 cells. Black-Right-Pointing-Pointer The proliferation of p21{sup -/-} HCT116 cells in galactose medium was significantly impaired. Black-Right-Pointing-Pointer p21 may play a role in maintaining proper mitochondrial mass and respiratory function. -- Abstract: p21{sup WAF1/CIP1} is a critical regulator of cell cycle progression. However, the role of p21 in mitochondrial function remains poorly understood. In this study, we examined the effect of p21 deficiency on mitochondrial function in HCT116 human colon cancer cells. We found that there was a significant increase in the mitochondrial mass of p21{sup -/-} HCT116 cells, as measured by 10-N-nonyl-acridine orange staining, as well as an increase in the mitochondrial DNA content. In contrast, p53{sup -/-} cells had a mitochondrial mass comparable to that of wild-type HCT116 cells. In addition, the expression levels of the mitochondrial biogenesis regulators PGC-1{alpha} and TFAM and AMPK activity were also elevated in p21{sup -/-} cells, indicating that p21 deficiency induces the rate of mitochondrial biogenesis through the AMPK-PGC-1{alpha} axis. However, the increase in mitochondrial biogenesis in p21{sup -/-} cells did not accompany an increase in the cellular steady-state level of ATP. Furthermore, p21{sup -/-} cells exhibited significant proliferation impairment in galactose medium, suggesting that p21 deficiency induces a defect in the mitochondrial respiratory chain in HCT116 cells. Taken together, our results suggest that the loss of p21 results in an aberrant increase in the mitochondrial mass and in mitochondrial dysfunction in HCT116 cells, indicating that p21 is required to maintain proper mitochondrial mass and respiratory function.

  9. Isotope fractionation

    NASA Astrophysics Data System (ADS)

    Bell, Peter M.

    A rash of new controversy has emerged around the subject of mass-independent isotope fractionation effects, particularly in the case of the oxygen isotopes. To be sure, the controversy has been around for awhile, but it has been given new impetus by the results of a recent study by Mark H. Thiemens and John E. Heidenreich III of the University of California, San Diego (Science, March 4, 1983).Gustav Arrhenius has been trying to convince the planetary science community that chemical effects in isotope fractionation processes could explain observations in meteorites that appear to be outside of the traditionally understood mass-dependent fractionations (G. Arrhenius, J . L. McCrumb, and N. F. Friedman, Astrophys. Space Sci, 65, 297, 1974). Robert Clayton had made the basic observations of oxygen in carbonaceous chondrites that the slope of the δ17 versus δ18 line was 1 instead of the slope of ½ characteristic of terrestrial rocks and lunar samples (Ann. Rev. Nucl. Part. Sci., 28, 501, 1978). The mass-independent effects were ascribed to the apparent contribution of an ancient presolar system component of O16.

  10. Conceptual design and programmatics studies of space station accommodations for Life Sciences Research Facilities (LSRF)

    NASA Technical Reports Server (NTRS)

    1985-01-01

    Conceptual designs and programmatics of the space station accommodations for the Life Sciences Research Facilities (LSRF) are presented. The animal ECLSS system for the LSRF provides temperature-humidity control, air circulation, and life support functions for experimental subjects. Three ECLSS were studied. All configurations presented satisfy the science requirements for: animal holding facilities with bioisolation; facilities interchangeable to hold rodents, small primates, and plants; metabolic cages interchangeable with standard holding cages; holding facilities adaptable to restrained large primates and rodent breeding/nesting cages; volume for the specified instruments; enclosed ferm-free workbench for manipulation of animals and chemical procedures; freezers for specimen storage until return; and centrifuge to maintain animals and plants at fractional g to 1 g or more, with potential for accommodating humans for short time intervals.

  11. Effect of Vision Therapy on Accommodation in Myopic Chinese Children

    PubMed Central

    Ma, Martin Ming-Leung; Scheiman, Mitchell; Su, Cuiyun

    2016-01-01

    Introduction. We evaluated the effectiveness of office-based accommodative/vergence therapy (OBAVT) with home reinforcement to improve accommodative function in myopic children with poor accommodative response. Methods. This was a prospective unmasked pilot study. 14 Chinese myopic children aged 8 to 12 years with at least 1 D of lag of accommodation were enrolled. All subjects received 12 weeks of 60-minute office-based accommodative/vergence therapy (OBAVT) with home reinforcement. Primary outcome measure was the change in monocular lag of accommodation from baseline visit to 12-week visit measured by Shinnipon open-field autorefractor. Secondary outcome measures were the changes in accommodative amplitude and monocular accommodative facility. Results. All participants completed the study. The lag of accommodation at baseline visit was 1.29 ± 0.21 D and it was reduced to 0.84 ± 0.19 D at 12-week visit. This difference (−0.46 ± 0.22 D; 95% confidence interval: −0.33 to −0.58 D) is statistically significant (p < 0.0001). OBAVT also increased the amplitude and facility by 3.66 ± 3.36 D (p = 0.0013; 95% confidence interval: 1.72 to 5.60 D) and 10.9 ± 4.8 cpm (p < 0.0001; 95% confidence interval: 8.1 to 13.6 cpm), respectively. Conclusion. Standardized 12 weeks of OBAVT with home reinforcement is able to significantly reduce monocular lag of accommodation and increase monocular accommodative amplitude and facility. A randomized clinical trial designed to investigate the effect of vision therapy on myopia progression is warranted. PMID:28097018

  12. Elastically Accommodated Grain-Boundary Sliding: New Insights from Experiment and Modeling

    NASA Astrophysics Data System (ADS)

    Jackson, I.; Skelton, R.; Faul, U.; Morris, S.

    2012-12-01

    According to the classic Raj-Ashby theory of grain-boundary sliding, elastic behaviour breaks down at sufficiently high temperatures and appropriate timescales of shear stress application - by sliding on boundaries of relatively low viscosity, accommodated, in the first instance by elastic distortion of the neighbouring grains. The distribution of normal stress across the slipped boundary is responsible for such elastic deformation, and provides the restoring force for recoverable (anelastic) behaviour. Only at higher temperatures and longer time scales, is it possible for appreciable diffusion to erode this distribution of boundary normal stress and progressively allow the additional permanent strains of diffusionally accommodated sliding. Separate characteristic timescales for elastically and diffusionally accommodated sliding, with distinctive dependencies on temperature and grain size, are difficult to reconcile with control of the full spectrum of viscoelastic behaviour by a single master variable, as has been widely advocated. However, in experimental studies of pure polycrystalline materials, the strain-energy dissipation peak predicted for elastically accommodated sliding has proved to be elusive. In a recent attempt to reconcile the results of forced-oscillation experiments with the predictions of the theory of grain-boundary sliding (Lee, Morris & Wilkening, Proc. R. Soc. A, 2011), a dissipation plateau at moderate temperatures for fine-grained polycrystalline olivine has been tentatively attributed to elastically accommodated sliding (Jackson & Faul, Phys. Earth Planet. Inter., 2010). Here we will examine two further lines of evidence concerning the nature of the transition between elastic and viscoelastic behaviour in polycrystalline olivine: (i) a re-assessment of torsional microcreep data that constrain the recoverable fraction of the non-elastic strain; and (ii) closer scrutiny of the mildly non-elastic behaviour observed at moderate temperatures

  13. ATHENA: system studies and optics accommodation

    NASA Astrophysics Data System (ADS)

    Ayre, M.; Bavdaz, M.; Ferreira, I.; Wille, E.; Fransen, S.; Stefanescu, A.; Linder, M.

    2016-07-01

    ATHENA is currently in Phase A, with a view to adoption upon a successful Mission Adoption Review in 2019/2020. After a brief presentation of the reference spacecraft (SC) design, this paper will focus on the functional and environmental requirements, the thermo-mechanical design and the Assembly, Integration, Verification & Test (AIVT) considerations related to housing the Silicon Pore Optics (SPO) Mirror Modules (MM) in the very large Mirror Assembly Module (MAM). Initially functional requirements on the MM accommodation are presented, with the Effective Area and Half Energy Width (HEW) requirements leading to a MAM comprising (depending on final mirror size selected) between 700-1000 MMs, co-aligned with exquisite accuracy to provide a common focus. A preliminary HEW budget allocated across the main error-contributors is presented, and this is then used as a reference to derive subsequent requirements and engineering considerations, including: The procedures and technologies for MM-integration into the Mirror Structure (MS) to achieve the required alignment accuracies in a timely manner; stiffness requirements and handling scheme required to constrain deformation under gravity during x-ray testing; temperature control to constrain thermo-elastic deformation during flight; and the role of the Instrument Switching Mechanism (ISM) in constraining HEW and Effective Area errors. Next, we present the key environmental requirements of the MMs, and the need to minimise shock-loading of the MMs is stressed. Methods to achieve this Ø are presented, including: Selection of a large clamp-band launch vehicle interface (LV I/F); lengthening of the shock-path from the LV I/F to the MAM I/F; modal-tuning of the MAM to act as a low-pass filter during launch shock events; use of low-shock HDRMs for the MAM; and the possibility to deploy a passive vibration solution at the LV I/F to reduce loads.

  14. Accommodation to an Unlikely Episodic State

    PubMed Central

    Clifton, Charles; Frazier, Lyn

    2015-01-01

    Mini-discourses like (ia) seem slightly odd compared to their counterparts containing a conjunction (ib). (i) a. Speaker A: John or Bill left. Speaker B: Sam did too. b. Speaker A: John and Bill left. Speaker B: Sam did too. One possibility is that or in Speaker A's utterance in (ia) raises the potential Question Under Discussion (QUD) whether it was John or Bill who left and Speaker B's reply fails to address this QUD. A different possibility is that the epistemic state of the speaker of (ia) is somewhat unlikely or uneven: the speaker knows that someone left, and that it was John or Bill, but doesn't know which one. The results of four acceptability judgment studies confirmed that (ia) is less good or coherent than (ib) (Experiment 1), but not due to failure to address the QUD implicitly introduced by the disjunction because the penalty for disjunction persisted even in the presence of a different overt QUD (Experiment 2) and even when there was no reply to Speaker A (Experiment 3). The hypothesis that accommodating an unusual epistemic state might underlie the lower acceptability of disjunction was supported by the fact that the disjunction penalty is larger in past tense discourses than in future discourses, where partial knowledge of events is the norm (Experiment 4). The results of an eye tracking study revealed a penalty for disjunction relative to conjunction that was significantly smaller when a lead in (I wonder if it was…) explicitly introduced the disjunction. This interaction (connective X lead in) appeared in early measures on the disjunctive phrase itself, suggesting that the input is related to an inferred epistemic state of the speaker in a rapid and ongoing fashion. PMID:26568651

  15. 46 CFR 127.260 - Ventilation for accommodations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... vessel of 100 or more gross tons must be provided with a mechanical ventilation system unless the... 46 Shipping 4 2010-10-01 2010-10-01 false Ventilation for accommodations. 127.260 Section 127.260... ARRANGEMENTS Particular Construction and Arrangements § 127.260 Ventilation for accommodations. (a)...

  16. 46 CFR 190.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Sleeping accommodations. 190.20-20 Section 190.20-20... CONSTRUCTION AND ARRANGEMENT Accomodations for Officers, Crew, and Scientific Personnel § 190.20-20 Sleeping...) Sleeping accommodations for the crew must be divided into rooms, no one of which must berth more than...

  17. 46 CFR 190.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Sleeping accommodations. 190.20-20 Section 190.20-20... CONSTRUCTION AND ARRANGEMENT Accomodations for Officers, Crew, and Scientific Personnel § 190.20-20 Sleeping...) Sleeping accommodations for the crew must be divided into rooms, no one of which must berth more than...

  18. 36 CFR 910.34 - Accommodations for the physically handicapped.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Accommodations for the... § 910.34 Accommodations for the physically handicapped. (a) Every development shall incorporate features... Standard Specifications for Making Buildings and Facilities Accessible to, and Usable by the...

  19. Religious Observance Accommodation in Ontario Universities. Discussion Paper.

    ERIC Educational Resources Information Center

    Reed, Carole Ann

    This paper highlights the religious accommodations that Ontario (Canada) universities have undertaken to create an inclusive, supportive learning community for all students, faculty, and staff. It outlines the demographic changes and public policy surrounding religious accommodation issues in Canada and in Ontario in particular, focusing on the…

  20. 46 CFR 127.260 - Ventilation for accommodations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Ventilation for accommodations. 127.260 Section 127.260... ARRANGEMENTS Particular Construction and Arrangements § 127.260 Ventilation for accommodations. (a) Each... vessel of 100 or more gross tons must be provided with a mechanical ventilation system unless...

  1. 46 CFR 127.260 - Ventilation for accommodations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Ventilation for accommodations. 127.260 Section 127.260... ARRANGEMENTS Particular Construction and Arrangements § 127.260 Ventilation for accommodations. (a) Each... vessel of 100 or more gross tons must be provided with a mechanical ventilation system unless...

  2. 46 CFR 127.260 - Ventilation for accommodations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Ventilation for accommodations. 127.260 Section 127.260... ARRANGEMENTS Particular Construction and Arrangements § 127.260 Ventilation for accommodations. (a) Each... vessel of 100 or more gross tons must be provided with a mechanical ventilation system unless...

  3. 46 CFR 127.270 - Location of accommodations and pilothouse.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... accommodations and chain lockers, cargo spaces, or machinery spaces. (f) No sounding tubes, or vents from fuel-oil or cargo-oil tanks may open into accommodations for crew members or offshore workers, except that sounding tubes may open into passageways. (g) No access openings from fuel-oil or cargo-oil tanks may...

  4. 46 CFR 127.270 - Location of accommodations and pilothouse.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... accommodations and chain lockers, cargo spaces, or machinery spaces. (f) No sounding tubes, or vents from fuel-oil or cargo-oil tanks may open into accommodations for crew members or offshore workers, except that sounding tubes may open into passageways. (g) No access openings from fuel-oil or cargo-oil tanks may...

  5. 46 CFR 127.270 - Location of accommodations and pilothouse.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... accommodations and chain lockers, cargo spaces, or machinery spaces. (f) No sounding tubes, or vents from fuel-oil or cargo-oil tanks may open into accommodations for crew members or offshore workers, except that sounding tubes may open into passageways. (g) No access openings from fuel-oil or cargo-oil tanks may...

  6. 46 CFR 127.270 - Location of accommodations and pilothouse.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... accommodations and chain lockers, cargo spaces, or machinery spaces. (f) No sounding tubes, or vents from fuel-oil or cargo-oil tanks may open into accommodations for crew members or offshore workers, except that sounding tubes may open into passageways. (g) No access openings from fuel-oil or cargo-oil tanks may...

  7. 46 CFR 154.325 - Accommodation, service, and control spaces.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 5 2012-10-01 2012-10-01 false Accommodation, service, and control spaces. 154.325 Section 154.325 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS... system, required by this part to have a secondary barrier, is separated from any accommodation,...

  8. Evaluating Computer-Based Test Accommodations for English Learners

    ERIC Educational Resources Information Center

    Roohr, Katrina Crotts; Sireci, Stephen G.

    2017-01-01

    Test accommodations for English learners (ELs) are intended to reduce the language barrier and level the playing field, allowing ELs to better demonstrate their true proficiencies. Computer-based accommodations for ELs show promising results for leveling that field while also providing us with additional data to more closely investigate the…

  9. 46 CFR 127.270 - Location of accommodations and pilothouse.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Section 127.270 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS... waterline. (d) No hawse pipe or chain pipe may pass through accommodations for crew members or offshore... accommodations and chain lockers, cargo spaces, or machinery spaces. (f) No sounding tubes, or vents from...

  10. 24 CFR 966.7 - Accommodation of persons with disabilities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Accommodation of persons with disabilities. 966.7 Section 966.7 Housing and Urban Development Regulations Relating to Housing and Urban... Requirements § 966.7 Accommodation of persons with disabilities. (a) For all aspects of the lease and...

  11. Family Accommodation in Pediatric Obsessive-Compulsive Disorder

    ERIC Educational Resources Information Center

    Storch, Eric A.; Geffken, Gary R.; Merlo, Lisa J.; Jacob, Marni L.; Murphy, Tanya K.; Goodman, Wayne K.; Larson, Michael J.; Fernandez, Melanie; Grabill, Kristen

    2007-01-01

    Despite the importance of the family in the treatment of pediatric obsessive-compulsive disorder (OCD), relatively little empirical attention has been directed to family accommodation of symptoms. This study examined the relations among family accommodation, OCD symptom severity, functional impairment, and internalizing and externalizing behavior…

  12. 46 CFR 190.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Sleeping accommodations. 190.20-20 Section 190.20-20... CONSTRUCTION AND ARRANGEMENT Accomodations for Officers, Crew, and Scientific Personnel § 190.20-20 Sleeping...) Sleeping accommodations for the crew must be divided into rooms, no one of which must berth more than...

  13. 46 CFR 190.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Sleeping accommodations. 190.20-20 Section 190.20-20... CONSTRUCTION AND ARRANGEMENT Accomodations for Officers, Crew, and Scientific Personnel § 190.20-20 Sleeping...) Sleeping accommodations for the crew must be divided into rooms, no one of which must berth more than...

  14. 46 CFR 190.20-20 - Sleeping accommodations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Sleeping accommodations. 190.20-20 Section 190.20-20... CONSTRUCTION AND ARRANGEMENT Accomodations for Officers, Crew, and Scientific Personnel § 190.20-20 Sleeping...) Sleeping accommodations for the crew must be divided into rooms, no one of which must berth more than...

  15. Regional Sign Language Varieties in Contact: Investigating Patterns of Accommodation

    ERIC Educational Resources Information Center

    Stamp, Rose; Schembri, Adam; Evans, Bronwen G.; Cormier, Kearsy

    2016-01-01

    Short-term linguistic accommodation has been observed in a number of spoken language studies. The first of its kind in sign language research, this study aims to investigate the effects of regional varieties in contact and lexical accommodation in British Sign Language (BSL). Twenty-five participants were recruited from Belfast, Glasgow,…

  16. Accommodating World Englishes in Developing EFL Learners' Oral Communication

    ERIC Educational Resources Information Center

    Mukminatien, Nur

    2012-01-01

    This article aims to discuss issues of World Englishes (WEs) and the implications in ELT. It explores the extent to which WEs are taken into account as emerging English varieties different from inner circle varieties, how WEs should be accommodated by English teachers, and which standard to adopt to accommodate learner's linguistic needs for…

  17. School Boarding Accommodation. A Design Guide. Building Bulletin 84.

    ERIC Educational Resources Information Center

    Wilson, Jeremy; Lloyd-Jones, Liz; Carden, Tom; Daniels, Richard; Fitzgerald, Brian; Maddick, Jenny

    This bulletin contains on-statutory guidance describing good practice and its implications for boarding accommodation to assist those responsible for briefing and designing boarding accommodations across all school sectors. It offers practical advice and illustrates examples that reflect changes in provision which are thought to be acceptable and…

  18. Examining Student Factors in Sources of Setting Accommodation DIF

    ERIC Educational Resources Information Center

    Lin, Pei-Ying; Lin, Yu-Cheng

    2014-01-01

    This exploratory study investigated potential sources of setting accommodation resulting in differential item functioning (DIF) on math and reading assessments for examinees with varied learning characteristics. The examinees were those who participated in large-scale assessments and were tested in either standardized or accommodated testing…

  19. Effects of Learning Style Accommodation on Achievement of Second Graders.

    ERIC Educational Resources Information Center

    Knight, Carol Bugg; Halpin, Gerald; Halpin, Glennelle

    1996-01-01

    Whether grades earned in reading, mathematics, and language by 158 second graders when learning environmental accommodations were made in the areas of light, sound, temperature, design, and mobility differed from grades of control group students without these accommodations was studied. Control group students had higher mathematics and language…

  20. Effects of Learning Style Accommodation on Achievement of Second Graders.

    ERIC Educational Resources Information Center

    Knight, Carol Bugg

    The purpose of this study was to devise an instructional model accommodating students' learning styles in the following areas: sound, light, temperature, design, and mobility. Specifically, this study determined if students in an experimental group with environmental accommodations to their preferred modes of learning differed from students in a…

  1. Transition to Postsecondary: New Documentation Guidance for Access to Accommodations

    ERIC Educational Resources Information Center

    Klotz, Mary Beth

    2012-01-01

    The Association on Higher Education and Disability (AHEAD) recently developed a conceptual framework that substantially revises its guidance for disability documentation for accommodations in higher education settings. This new document, "Supporting Accommodation Requests: Guidance on Documentation Practices," was written in response to the…

  2. Impact of Workstation Accommodation on Fatigue and Performance

    DTIC Science & Technology

    2006-12-01

    this study. The present research focuses on the biodynamic impact of anthropometric accommodation of a dual- monitor computer workstation on an...Biometric Response Cognitive Response Good Anthropometric Accommodation Sustainable & Optimal Muscular...Activity, Low Levels of Subjective Discomfort Sustainable & Optimal Levels of Cerebral Oxygenation & Cognitive Performance Poor Anthropometric

  3. 14 CFR 1204.1510 - Efforts to accommodate intergovernmental concerns.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Efforts to accommodate intergovernmental concerns. 1204.1510 Section 1204.1510 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION... Programs and Activities § 1204.1510 Efforts to accommodate intergovernmental concerns. (a) If a...

  4. An Accommodations Model for the Secondary Inclusive Classroom

    ERIC Educational Resources Information Center

    Scanlon, David; Baker, Diana

    2012-01-01

    Despite expectations for accommodations in inclusive classrooms, little guidance for effective practice is available. Most accommodations policies and evidence-based practices address assessments. High school regular and special educators collaborated in focus groups to articulate a model based on their practices and perceptions of best practice.…

  5. 24 CFR 966.7 - Accommodation of persons with disabilities.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 4 2012-04-01 2012-04-01 false Accommodation of persons with disabilities. 966.7 Section 966.7 Housing and Urban Development REGULATIONS RELATING TO HOUSING AND URBAN... Requirements § 966.7 Accommodation of persons with disabilities. (a) For all aspects of the lease and...

  6. 24 CFR 966.7 - Accommodation of persons with disabilities.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 4 2013-04-01 2013-04-01 false Accommodation of persons with disabilities. 966.7 Section 966.7 Housing and Urban Development REGULATIONS RELATING TO HOUSING AND URBAN... Requirements § 966.7 Accommodation of persons with disabilities. (a) For all aspects of the lease and...

  7. 24 CFR 966.7 - Accommodation of persons with disabilities.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 4 2014-04-01 2014-04-01 false Accommodation of persons with disabilities. 966.7 Section 966.7 Housing and Urban Development REGULATIONS RELATING TO HOUSING AND URBAN... Requirements § 966.7 Accommodation of persons with disabilities. (a) For all aspects of the lease and...

  8. 24 CFR 966.7 - Accommodation of persons with disabilities.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 4 2011-04-01 2011-04-01 false Accommodation of persons with disabilities. 966.7 Section 966.7 Housing and Urban Development REGULATIONS RELATING TO HOUSING AND URBAN... Requirements § 966.7 Accommodation of persons with disabilities. (a) For all aspects of the lease and...

  9. Spacecraft thermal energy accommodation from atomic recombination

    NASA Technical Reports Server (NTRS)

    Carleton, Karen L.; Marinelli, William J.

    1991-01-01

    Measurements of atomic recombination probabilities important in determining energy release to reusable spacecraft thermal protection surfaces during reentry are presented. An experimental apparatus constructed to examine recombination of atomic oxygen from thermal protection and reference materials at reentry temperatures is described. The materials are examined under ultrahigh vacuum conditions to develop and maintain well characterized surface conditions that are free of contamination. When compared with stagnation point heat transfer measurements performed in arc jet facilities, these measurements indicate that a significant fraction of the excess energy available from atom recombination is removed from the surface as metastable O2.

  10. Cultural value orientations, internalized homophobia, and accommodation in romantic relationships.

    PubMed

    Gaines, Stanley O; Henderson, Michael C; Kim, Mary; Gilstrap, Samuel; Yi, Jennifer; Rusbult, Caryl E; Hardin, Deletha P; Gaertner, Lowell

    2005-01-01

    In the present study, we examined the impact of cultural value orientations (i.e., the personally oriented value of individualism, and the socially oriented values of collectivism, familism, romanticism, and spiritualism) on accommodation (i.e., voice and loyalty, rather than exit and neglect, responses to partners' anger or criticism) in heterosexual and gay relationships; and we examined the impact of internalized homophobia (i.e., attitudes toward self, other, and disclosure) on accommodation specifically in gay relationships. A total of 262 heterosexuals (102 men and 162 women) and 857 gays (474 men and 383 women) participated in the present study. Consistent with hypotheses, among heterosexuals and gays, socially oriented values were significantly and positively related to accommodation (whereas the personally oriented value of individualism was unrelated to accommodation); and among gays in particular, internalized homophobia was significantly and negatively related to accommodation. Implications for the study of heterosexual and gay relationships are discussed.

  11. Restoration of accommodation: surgical options for correction of presbyopia

    PubMed Central

    Glasser, Adrian

    2010-01-01

    Accommodation is a dioptric change in the power of the eye to see clearly at near. Ciliary muscle contraction causes a release in zonular tension at the lens equator, which permits the elastic capsule to mould the young lens into an accommodated form. Presbyopia, the gradual age-related loss of accommodation, occurs primarily through a gradual age-related stiffening of the lens. While there are many possible options for relieving the symptoms of presbyopia, only relatively recently has consideration been given to surgical restoration of accommodation to the presbyopic eye. To understand how this might be achieved, it is necessary to understand the accommodative anatomy, the mechanism of accommodation and the causes of presbyopia. A variety of different kinds of surgical procedures has been considered for restoring accommodation to the presbyopic eye, including surgical expansion of the sclera, using femtosecond lasers to treat the lens or with so-called accommodative intraocular lenses (IOLs). Evidence suggests that scleral expansion cannot and does not restore accommodation. Laser treatments of the lens are in their early infancy. Development and testing of accommodative IOLs are proliferating. They are designed to produce a myopic refractive change in the eye in response to ciliary muscle contraction either through a movement of an optic or through a change in surface curvature. Three general design principles are being considered. These are single optic IOLs that rely on a forward shift of the optic, dual optic IOLs that rely on an increased separation between the two optics, or IOLs that permit a change in surface curvature to produce an increase in optical power in response to ciliary muscle contraction. Several of these different IOLs are available and being used clinically, while many are still in research and development. PMID:18399800

  12. Accommodating electric propulsion on SMART-1

    NASA Astrophysics Data System (ADS)

    Kugelberg, Joakim; Bodin, Per; Persson, Staffan; Rathsman, Peter

    2004-07-01

    how the problems related to the accommodation of electric propulsion have been solved during design and planning of the project.

  13. Zac1, an Sp1-like protein, regulates human p21{sup WAF1/Cip1} gene expression in HeLa cells

    SciTech Connect

    Liu, Pei-Yao; Hsieh, Tsai-Yuan; Liu, Shu-Ting; Chang, Yung-Lung; Lin, Wei-Shiang; Wang, Wei-Ming; Huang, Shih-Ming

    2011-12-10

    Zac1 functions as both a transcription factor and a transcriptional cofactor for p53, nuclear receptors (NRs) and NR coactivators. Zac1 might also act as a transcriptional repressor via the recruitment of histone deacetylase 1 (HDAC1). The ability of Zac1 to interact directly with GC-specific elements indicates that Zac1 possibly binds to Sp1-responsive elements. In the present study, our data show that Zac1 is able to interact directly with the Sp1-responsive element in the p21{sup WAF1/Cip1} gene promoter and enhance the transactivation activity of Sp1 through direct physical interaction. Our data further demonstrate that Zac1 might enhance Sp1-specific promoter activity by interacting with the Sp1-responsive element, affecting the transactivation activity of Sp1 via a protein-protein interaction, or competing the HDAC1 protein away from the pre-existing Sp1/HDAC1 complex. Finally, the synergistic regulation of p21{sup WAF1/Cip1} gene expression by Zac1 and Sp1 is mediated by endogenous p53 protein and p53-responsive elements in HeLa cells. Our work suggests that Zac1 might serve as an Sp1-like protein that directly interacts with the Sp1-responsive element to oligomerize with and/or to coactivate Sp1.

  14. Subcellular Targeting of p33ING1b by Phosphorylation-Dependent 14-3-3 Binding Regulates p21WAF1 Expression

    PubMed Central

    Gong, Wei; Russell, Michael; Suzuki, Keiko; Riabowol, Karl

    2006-01-01

    ING1 is a type II tumor suppressor that affects cell growth, stress signaling, apoptosis, and DNA repair by altering chromatin structure and regulating transcription. Decreased ING1 expression is seen in several human cancers, and mislocalization has been noted in diverse types of cancer cells. Aberrant targeting may, therefore, functionally inactivate ING1. Bioinformatics analysis identified a sequence between the nuclear localization sequence and plant homeodomain domains of ING1 that closely matched the binding motif of 14-3-3 proteins that target cargo proteins to specific subcellular locales. We find that the widely expressed p33ING1b splicing isoform of ING1 interacts with members of the 14-3-3 family of proteins and that this interaction is regulated by the phosphorylation status of ING1. 14-3-3 binding resulted in significant amounts of p33ING1b protein being tethered in the cytoplasm. As shown previously, ectopic expression of p33ING1b increased levels of the p21Waf1 cyclin-dependent kinase inhibitor upon UV-induced DNA damage. Overexpression of 14-3-3 inhibited the up-regulation of p21Waf1 by p33ING1b, consistent with the idea that mislocalization blocks at least one of ING1's biological activities. These data support the idea that the 14-3-3 proteins play a crucial role in regulating the activity of p33ING1b by directing its subcellular localization. PMID:16581770

  15. The Forkhead Transcription Factor FOXP2 Is Required for Regulation of p21WAF1/CIP1 in 143B Osteosarcoma Cell Growth Arrest.

    PubMed

    Gascoyne, Duncan M; Spearman, Hayley; Lyne, Linden; Puliyadi, Rathi; Perez-Alcantara, Marta; Coulton, Les; Fisher, Simon E; Croucher, Peter I; Banham, Alison H

    2015-01-01

    Mutations of the forkhead transcription factor FOXP2 gene have been implicated in inherited speech-and-language disorders, and specific Foxp2 expression patterns in neuronal populations and neuronal phenotypes arising from Foxp2 disruption have been described. However, molecular functions of FOXP2 are not completely understood. Here we report a requirement for FOXP2 in growth arrest of the osteosarcoma cell line 143B. We observed endogenous expression of this transcription factor both transiently in normally developing murine osteoblasts and constitutively in human SAOS-2 osteosarcoma cells blocked in early osteoblast development. Critically, we demonstrate that in 143B osteosarcoma cells with minimal endogenous expression, FOXP2 induced by growth arrest is required for up-regulation of p21WAF1/CIP1. Upon growth factor withdrawal, FOXP2 induction occurs rapidly and precedes p21WAF1/CIP1 activation. Additionally, FOXP2 expression could be induced by MAPK pathway inhibition in growth-arrested 143B cells, but not in traditional cell line models of osteoblast differentiation (MG-63, C2C12, MC3T3-E1). Our data are consistent with a model in which transient upregulation of Foxp2 in pre-osteoblast mesenchymal cells regulates a p21-dependent growth arrest checkpoint, which may have implications for normal mesenchymal and osteosarcoma biology.

  16. Microgravity Flight: Accommodating Non-Human Primates

    NASA Technical Reports Server (NTRS)

    Dalton, Bonnie P.; Searby, Nancy; Ostrach, Louis

    1995-01-01

    thermoregulation, muscular, and cardiac responses to weightlessness. In contrast, the five completed Cosmos/Bion flights, lacked the metabolic samples and behavioral task monitoring, but did facilitate studies of the neurovestibular system during several of the flights. The RRF accommodated two adult 8-11 kg rhesus monkeys, while the Russian experiments and hardware were configured for a younger animal in the 44 kg range. Both the American and Russian hardware maintained a controlled environmental system, specifically temperature, humidity, a timed lighting cycle, and had means for providing food and fluids to the animal(s). Crew availability during a Shuttle mission was to be an optimal condition for retrieval and refrigeration of the animal urine samples along with a manual calcein injection which could lead to greater understanding of bone calcium incorporation. A special portable bioisolation glove box was under development to support this aspect of the experiment profile along with the capability of any contingency human intervention. As a result of recent U.S./Russian negotiations, funding for Space Station, and a series of other events, the SLS-3 mission was cancelled and applicable Rhesus Project experiments incorporated into the Russian Bion 11 and 12 missions. A presentation of the RRF and COSMOS/Bion rhesus hardware is presented along with current plans for the hardware.

  17. Microgravity Flight - Accommodating Non-Human Primates

    NASA Technical Reports Server (NTRS)

    Dalton, Bonnie P.; Searby, Nancy; Ostrach, Louis

    1994-01-01

    thermoregulation, muscular, and cardiac responses to weightlessness. In contrast, the five completed Cosmos/Bion flights, lacked the metabolic samples and behavioral task monitoring, but did facilitate studies of the neurovestibular system during several of the flights. The RRF accommodated two adult 8-11 kg rhesus monkeys, while the Russian experiments and hardware were configured for a younger animal in the 44 kg range. Both the American and Russian hardware maintained a controlled environmental system, specifically temperature, humidity, a timed lighting cycle, and had means for providing food and fluids to the animal(s). Crew availability during a Shuttle mission was to be an optimal condition for retrieval and refrigeration of the animal urine samples along with a manual calcein injection which could lead to greater understanding of bone calcium incorporation. A special portable bioisolation glove box was under development to support this aspect of the experiment profile along with the capability of any contingency human intervention. As a result of recent U.S./Russian negotiations, funding for Space Station, and a series of other events, the SLS-3 mission was cancelled and applicable Rhesus Project experiments incorporated into the Russian Bion 11 and 12 missions. A presentation of the RRF and COSMOS/Bion rhesus hardware is presented along with current plans for the hardware.

  18. Circadian rhythms of visual accommodation responses and physiological correlations.

    NASA Technical Reports Server (NTRS)

    Murphy, M. R.; Randle, R. J.; Williams, B. A.

    1972-01-01

    Use of a recently developed servocontrolled infrared optometer to continuously record the state of monocular focus while subjects viewed a visual target for which the stimulus to focus was systematically varied. Calculated parameters form recorded data - e.g., speeds of accommodation to approaching and receding targets, magnitude of accommodation to step changes in target distance, and amplitude and phase lag of response to sinusoidally varying stimuli were submitted to periodicity analyses. Ear canal temperature (ECT) and heart rate (HR) rhythms were also recorded for physiological correlation with accommodation rhythms. HR demonstrated a 24-hr rhythm, but ECT data did not.

  19. Assessment of the photoenhanced toxicity of a weathered oil to the tidewater silverside

    USGS Publications Warehouse

    Little, Edward E.; Cleveland, Laverne; Calfee, Robin D.; Barron, Mace G.

    2000-01-01

    Studies were conducted to determine the interactive toxicity of a water-accommodated fraction (WAF) of a weathered middle distillate petroleum and solar radiation to an estuarine organism, the tidewater silverside (Menidia beryllina). Juvenile silversides were monitored for survival and growth during a 7-d static renewal exposure to dilutions of WAFs of an environmentally weathered oil collected in the vicinity of an abandoned oil field in California. Ultraviolet (UV) treatments were based on incident sunlight intensity and spectra measured at this site. Exposure to UV alone was not lethal to the fish, and WAF in the absence of UV was toxic at the highest total petroleum hydrocarbon (TPH) concentration (3.03 mg/L) after 96 h of exposure. Water-accommodated fractions toxicity increased significantly with increasing UV irradiance and duration of exposure. The 7-d LC50 concentrations for the control, low, medium, and high irradiance were 2.84, 1.27, 0.93, and 0.51 mg/L TPH, respectively. Significant mortality occurred among fish previously exposed to WAF in the absence of irradiance, whereas WAF toxicity was unaffected by UV exposure prior to the toxicity test. Thus, the mode of action is a photosensitization of the accumulated petroleum residue rather than a photoactivation of WAF. Chemical analysis indicates that the WAF contains limited amounts of polycyclic aromatic hydrocarbons (PAHs) known to be photoenhanced, suggesting that other constituents may be responsible for the observed photoenhanced toxicity.

  20. The relationship between instructional and assessment accommodations in an inclusive state accountability system.

    PubMed

    Ysseldyke, J; Thurlow, M; Bielinski, J; House, A; Moody, M; Haigh, J

    2001-01-01

    We investigated the kinds of instructional and assessment accommodations students with disabilities receive, and the extent to which instructional accommodations match assessment accommodations. Most students who had IEPs in specific content areas received instructional accommodations in those areas, and there were no differences by disability type. We provide data on the specific types of accommodations used. Overall, students' assessment accommodations matched their instructional accommodations, though many students received testing accommodations that had not been received in instruction. Implications are discussed for IEP teams who make decisions about instructional and assessment accommodations.

  1. Naringin-induced p21WAF1-mediated G(1)-phase cell cycle arrest via activation of the Ras/Raf/ERK signaling pathway in vascular smooth muscle cells.

    PubMed

    Lee, Eo-Jin; Moon, Gi-Seong; Choi, Won-Seok; Kim, Wun-Jae; Moon, Sung-Kwon

    2008-12-01

    The flavonoid naringin has been shown to play a role in preventing the development of cardiovascular disease. However, the exact molecular mechanisms underlying the roles of integrated cell cycle regulation and MAPK signaling pathways in the regulation of naringin-induced inhibition of cell proliferation in vascular smooth muscle cells (VSMCs) remain to be identified. Naringin treatment resulted in significant growth inhibition and G(1)-phase cell cycle arrest mediated by induction of p53-independent p21WAF1 expression; expression of cyclins and CDKs in VSMCs was also down-regulated. In addition, among the pathways examined, blockade of ERK function inhibited naringin-dependent p21WAF1 expression, reversed naringin-mediated inhibition of cell proliferation and decreased cell cycle proteins. Moreover, naringin treatment increased both Ras and Raf activations. Transfection of cells with dominant negative Ras (RasN17) and Raf (RafS621A) mutant genes suppressed naringin-induced ERK activity and p21WAF1 expression. Finally, naringin-induced reduction in cell proliferation and cell cycle protein was abolished in the presence of RasN17 and RafS621A mutant genes. The Ras/Raf/ERK pathway participates in p21WAF1 induction, leading to a decrease in cyclin D1/CDK4 and cyclin E/CDK2 complexes and in naringin-dependent inhibition of cell growth. These novel and unexpected findings provide a theoretical basis for preventive use of flavonoids to the atherosclerosis disease.

  2. CGK733-induced LC3 II formation is positively associated with the expression of cyclin-dependent kinase inhibitor p21Waf1/Cip1 through modulation of the AMPK and PERK/CHOP signaling pathways.

    PubMed

    Wang, Yufeng; Kuramitsu, Yasuhiro; Baron, Byron; Kitagawa, Takao; Tokuda, Kazuhiro; Akada, Junko; Nakamura, Kazuyuki

    2015-11-24

    Microtubule-associated protein 1A/1B-light chain 3 (LC3)-II is essential for autophagosome formation and is widely used to monitor autophagic activity. We show that CGK733 induces LC3 II and LC3-puncta accumulation, which are not involved in the activation of autophagy. The treatment of CGK733 did not alter the autophagic flux and was unrelated to p62 degradation. Treatment with CGK733 activated the AMP-activated protein kinase (AMPK) and protein kinase RNA-like endoplasmic reticulum kinase/CCAAT-enhancer-binding protein homologous protein (PERK/CHOP) pathways and elevated the expression of p21Waf1/Cip1. Inhibition of both AMPK and PERK/CHOP pathways by siRNA or chemical inhibitor could block CGK733-induced p21Waf1/Cip1 expression as well as caspase-3 cleavage. Knockdown of LC3 B (but not LC3 A) abolished CGK733-triggered LC3 II accumulation and consequently diminished AMPK and PERK/CHOP activity as well as p21Waf1/Cip1 expression. Our results demonstrate that CGK733-triggered LC3 II formation is an initial event upstream of the AMPK and PERK/CHOP pathways, both of which control p21Waf1/Cip1 expression.

  3. Visual Accommodation, the Mandelbaum Effect, and Apparent Size.

    DTIC Science & Technology

    1979-11-01

    monkeys were awakened, accommodation immediately went to a far distance, followed by accurate accommodation to the - ambient stimuli. Similarly... ambient stimulation is present. Morgan (1957) proposes - two possible conditions in which visual stimuli are reduced to the minimum--in complete darkness...oj Physiology, 1941, 12, 720-733. Otero, J. M. and Duran, Continuacion del estudio de la miopia nocturna. Anales de i y Quimica , 1942, 38, 236-248

  4. Caffeine intake is associated with pupil dilation and enhanced accommodation.

    PubMed

    Abokyi, S; Owusu-Mensah, J; Osei, K A

    2017-04-01

    PurposeIt is purported that caffeine, an autonomic stimulant, affects visual performance. This study sought to assess whether caffeine intake was associated with changes in pupil size and/or amplitude of accommodation.Patients and methodsA double-masked, crossover study was conducted in 50 healthy subjects of age range 19 to 25 years. Subjects were randomized to treatments such that subjects consumed either 250 mg caffeine drink or vehicle on separate days. Amplitude of accommodation was measured by the push-up technique, and pupil size using a millimeter ruler fixed to a slit lamp biomicroscope in dim illumination (5 lux). Amplitude of accommodation and pupil size were taken at baseline, and at 30, 60 and 90 min time points post treatment. Repeated measures one-way ANOVA and paired t-test were used in analyzing data.ResultsAmplitude of accommodation and pupil size after caffeine intake were significantly greater than vehicle (P<0.001) at each time point. Consumption of the caffeine beverage was associated with significant increases in amplitude of accommodation and pupil size with time (P<0.001). Amplitude of accommodation rose from 12.4 (±2.2 D) at baseline to 15.8(±2.6 D) at 90 min. Similarly, pupil size increased from 3.4 (±0.4 mm) at baseline to 4.5 (±0.72 mm) at 90 min. Consumption of vehicle was not associated with increase in amplitude of accommodation or pupil size with time.ConclusionPupil size and accommodation are affected after ingestion of caffeine. This study suggests caffeine may have some influence on visual functions.

  5. Transcriptional inhibition of p21{sup WAF1/CIP1} gene (CDKN1) expression by survivin is at least partially p53-dependent: Evidence for survivin acting as a transcription factor or co-factor

    SciTech Connect

    Tang, Lei; Ling, Xiang; Liu, Wensheng; Das, Gokul M.; Li, Fengzhi

    2012-05-04

    Highlights: Black-Right-Pointing-Pointer Survivin inhibits the expression of p21 protein, mRNA and promoter activity. Black-Right-Pointing-Pointer Survivin neutralizes p53-induced p21 expression and promoter activity. Black-Right-Pointing-Pointer Survivin physically interacts with p53 in cancer cells. Black-Right-Pointing-Pointer Genetic silencing of endogenous survivin upregulates p21 in p53 wild type cancer cells. Black-Right-Pointing-Pointer Both p53 and survivin interacts on the two p53-binding sites in the p21 promoter. -- Abstract: Growing evidence suggests a role for the antiapoptotic protein survivin in promotion of cancer cell G1/S transition and proliferation. However, the underlying mechanism is unclear. Further, although upregulation of p21{sup WAF1/CIP1} by p53 plays an important role in p53-mediated cell G1 arrests in response to various distresses, it is unknown whether survivin plays a role in the regulation of p21{sup WAF1/CIP1} expression. Here, we report that exogenous expression of survivin in p53-wild type MCF-7 breast cancer cells inhibits the expression of p21{sup WAF1/CIP1} protein, mRNA and promoter activity, while the survivin C84A mutant and antisense failed to do so. Cotransfection experiments in the p53 mutant H1650 lung cancer cell line showed that survivin neutralizes p53-induced p21{sup WAF1/CIP1} expression and promoter activity. Importantly, genetically silencing of endogenous survivin using lentiviral survivin shRNA also enhances endogenous p21 in p53 wild type cancer cells, suggesting the physiological relevance of the fining. We further demonstrated that both p53 and survivin interacts on the two p53-binding sites in the p21{sup WAF1/CIP1} promoter (-2313 to -2212; -1452 to -1310), and survivin physically interacts with p53 in cancer cells. Together, we propose that survivin may act as a transcription factor or cofactor to interact with p53 on the p21{sup WAF1/CIP1} promoter leading to the inhibition of p21{sup WAF1/CIP1

  6. Accommodation of Symptoms in Anorexia Nervosa: A Qualitative Study.

    PubMed

    Fox, John R E; Whittlesea, Anna

    2016-06-17

    Anorexia nervosa (AN) continues to remain poorly understood within eating disorders. Recent research and theory have moved away from understanding its aetiological causes, addressing instead potential maintaining factors. This study is focused on interpersonal maintenance factors: the response of close others. Relatives of those with AN typically carry the main burden of care, and research has found high levels of carer distress and unmet needs. Recent theories have proposed this emotional impact to contribute to expressed emotion and other unhelpful caregiver interactions which inadvertently maintain AN. One such understudied response is accommodation, described as a 'process' whereby caregivers 'assist or participate' in symptomatic behaviours of the cared for individual. There is a dearth of research relating to accommodation within eating disorders, particularly qualitative accounts. This study utilized a grounded theory methodology to explore caregivers' responses to managing AN, focusing particularly on carers' experience of accommodation. Eight participants with experience of caring for an individual diagnosed with AN were interviewed. Participants were recruited from a national eating disorder charity and regional eating disorder service. A number of themes emerged, including the importance of caregivers' emotional resources in mediating accommodation responses. Low-perceived efficacy over AN contributed to caregiver burnout. Decreased emotional resources influenced a shift in caregiving aims conducive with accommodation. Nevertheless, carers perceived accommodation as counterproductive to recovery and consequently experienced internal conflict (cognitive dissonance). Dissonance was reduced using a number of cognitive and behavioural strategies. The implications of these findings are discussed with reference to existing literature. Copyright © 2016 John Wiley & Sons, Ltd.

  7. Development of seating accommodation models for soldiers in vehicles.

    PubMed

    Zerehsaz, Yaser; Jin, Jionghua Judy; Ebert, Sheila M; Reed, Matthew P

    2017-04-01

    Data from a previous study of soldier driving postures and seating positions were analysed to develop statistical models for defining accommodation of driver seating positions in military vehicles. Regression models were created for seating accommodation applicable to driver positions with a fixed heel point and a range of steering wheel locations in typical tactical vehicles. The models predict the driver-selected seat position as a function of population anthropometry and vehicle layout. These models are the first driver accommodation models considering the effects of body armor and body-borne gear. The obtained results can benefit the design of military vehicles, and the methods can also be extended to be utilised in the development of seating accommodation models for other driving environments where protective equipment affects driver seating posture, such as vehicles used by law-enforcement officers and firefighters. Practitioner Summary: A large-scale laboratory study of soldier driving posture and seating position was designed to focus on tactical vehicle (truck) designs. Regression techniques are utilised to develop accommodation models suitable for tactical vehicles. These are the first seating accommodation models based on soldier data to consider the effects of personal protective equipment and body-borne gear.

  8. Inhibition of MDA-MB-231 breast cancer cell proliferation and tumor growth by apigenin through induction of G2/M arrest and histone H3 acetylation-mediated p21(WAF1/CIP1) expression.

    PubMed

    Tseng, Tsui-Hwa; Chien, Ming-Hsien; Lin, Wea-Lung; Wen, Yu-Ching; Chow, Jyh-Ming; Chen, Chi-Kuan; Kuo, Tsang-Chih; Lee, Wei-Jiunn

    2017-02-01

    Apigenin (4',5,7-trihydroxyflavone), a flavonoid commonly found in fruits and vegetables, has anticancer properties in various malignant cancer cells. However, the molecular basis of the anticancer effect remains to be elucidated. In this study, we investigated the cellular mechanisms underlying the induction of cell cycle arrest by apigenin. Our results showed that apigenin at the nonapoptotic induction concentration inhibited cell proliferation and induced cell cycle arrest at the G2/M phase in the MDA-MB-231 breast cancer cell line. Immunoblot analysis indicated that apigenin suppressed the expression of cyclin A, cyclin B, and cyclin-dependent kinase-1 (CDK1), which control the G2-to-M phase transition in the cell cycle. In addition, apigenin upregulated p21(WAF1/CIP1) and increased the interaction of p21(WAF1/CIP1) with proliferating cell nuclear antigen (PCNA), which inhibits cell cycle progression. Furthermore, apigenin significantly inhibited histone deacetylase (HDAC) activity and induced histone H3 acetylation. The subsequent chromatin immunoprecipitation (ChIP) assay indicated that apigenin increased acetylation of histone H3 in the p21(WAF1/CIP1) promoter region, resulting in the increase of p21(WAF1/CIP1) transcription. In a tumor xenograft model, apigenin effectively delayed tumor growth. In these apigenin-treated tumors, we also observed reductions in the levels of cyclin A and cyclin B and increases in the levels of p21(WAF1/CIP1) and acetylated histone H3. These findings demonstrate for the first time that apigenin can be used in breast cancer prevention and treatment through epigenetic regulation. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 434-444, 2017.

  9. The role of p21(waf1/cip1) and p27(Kip1) in HDACi-mediated tumor cell death and cell cycle arrest in the Eμ-myc model of B-cell lymphoma.

    PubMed

    Newbold, A; Salmon, J M; Martin, B P; Stanley, K; Johnstone, R W

    2014-11-20

    Following the establishment of histone deacetylases (HDACs) as promising therapeutic targets for the reversal of aberrant epigenetic states associated with cancer, the development of HDAC inhibitors (HDACi) and their underlying mechanisms of action has been a significant area of scientific interest. HDACi induce diverse biological responses including the inhibition of cell proliferation by blocking progression through the G1 or G2/M phases of the cell cycle. As a putative tumor-suppressor protein, p21(waf1/cip1) influences cell proliferation by inhibiting the activity of cyclin-cyclin-dependent kinase (CDK) complexes at the G1/S and G2/M cell cycle checkpoints. HDACi transcriptionally activate CDKN1A, and it has been proposed that induction of p21(waf1/cip1) can determine if a cell undergoes apoptosis or cell cycle arrest following HDACi treatment. In the Eμ-myc transgenic mouse model of B-cell lymphoma, knockout of cdkn1a had no effect on disease latency, indicating that p21(waf1/cip1) did not function as a tumor suppressor in this system. Although HDACi robustly induced expression of p21(waf1/cip1) in wild-type Eμ-myc lymphomas, deletion of cdkn1a did not sensitize the lymphoma cells to HDACi-induced apoptosis and HDACi-induced cell cycle arrest still occurred. However, knockdown of cdkn1b in cdkn1a knockout lymphomas resulted in defective vorinostat-mediated arrest at G1/S indicating an essential role of p27(Kip1) in mediating this biological response to vorinostat. These data demonstrate that induction of cdkn1a does not regulate HDACi-mediated tumor cell apoptosis and refute the notion that p21(waf1/cip1) is an obligate mediator of HDACi-induced cell cycle arrest.

  10. Accommodation in young adults wearing aspheric multifocal soft contact lenses

    NASA Astrophysics Data System (ADS)

    Lindskoog Pettersson, Anna; Wahlberg Ramsay, Marika; Lundström, Linda; Rosén, Robert; Nilsson, Maria; Unsbo, Peter; Brautaset, Rune

    2011-11-01

    The aim of the present project was to investigate accommodative behavior in young adults and adolescents fitted with an aspheric multifocal (center distance) contact lens with focus on evaluating whether these lenses can be an alternative treatment for subjects in which a reduced level of blur and thereby accommodation in near vision is aimed at. Twenty normal subjects aged between 21 and 35 years participated in the study. Aberrometry was perfomed using a Zywave™ aberrometer, first on the uncorrected eyes of all subjects, and again while the subjects wore a multifocal contact lens with a +1.00 add. A Shin-Nippon N Vision-K 5001 Autoref-Keratometer was used to measure accommodative response with two different refractive corrections: (1) habitual spectacle correction only, and (2) habitual correction and a aspheric multifocal (center distance) contact lens. Four hours of adaptation to the lens was allowed. The lag when wearing only the habitual spectacles was compared with the lag while wearing both the habitual spectacles and the aspheric multifocal contact lens. The mean lag of accommodation for the subject group was 0.85 D (±0.57 SD) and 0.75 D (±0.52 SD) without and with the multifocal lens, respectively. Statistical analyses showed no difference in lag (t = 0.8479, p = 0.407) with and without the lens. In conclusion, young normal subjects do not relax accommodation when fitted with aspheric multifocal center distance lenses when the addition is +1.00. It is therefore unlikely that subjects with accommodative ability, in whom the treatment purpose is to reduce blur and thereby accommodation, can be effectively treated with such lenses.

  11. Accommodation and pupil responses to random-dot stereograms.

    PubMed

    Suryakumar, Rajaraman; Allison, Robert

    2016-01-01

    We investigated the dynamics of accommodative and pupillary responses to random-dot stereograms presented in crossed and uncrossed disparity in six visually normal young adult subjects (mean age=25.8±3.1 years). Accommodation and pupil measures were monitored monocularly with a custom built photorefraction system while subjects fixated at the center of a random-dot stereogram. On each trial, the stereogram initially depicted a flat plane and then changed to depict a sinusoidal corrugation in depth while fixation remained constant. Increase in disparity specified depth resulted in pupil constriction during both crossed and uncrossed disparity presentations. The change in pupil size between crossed and uncrossed disparity conditions was not significantly different (p>0.05). The change in pupil size was also accompanied by a small concomitant increase in accommodation. In addition, the dynamic properties of pupil responses varied as a function of their initial (starting) diameter. The finding that accommodation and pupil responses increased with disparity regardless of the sign of retinal disparity suggests that these responses were driven by apparent depth rather than shifts in mean simulated distance of the stimulus. Presumably the need for the increased depth of focus when viewing stimuli extended in depth results in pupil constriction which also results in a concomitant change in accommodation. Starting position effects in pupil response confirm the non-linearity in the operating range of the pupil.

  12. Short-term induction of assimilation and accommodation.

    PubMed

    Leipold, Bernhard; Bermeitinger, Christina; Greve, Werner; Meyer, Birgit; Arnold, Manuel; Pielniok, Marianna

    2014-01-01

    The dual-process model of developmental regulation distinguishes two processes of self-regulation (assimilation = tenacious goal pursuit, and accommodation = flexible goal adjustment) that depend on differing conditions, but both contribute to successful development. Four experiments were conducted to investigate whether assimilation and accommodation can be induced or at least shifted by sensorimotor and cognitive manipulations. Experiment 1 investigated the relation between body manipulation and self-regulation. It was shown that assimilation could be triggered when participants were asked to hold on to golf balls as compared to being asked to drop them. Experiment 2 showed that a semantic priming of "let go" or "hold on" via instructions influenced the processes of self-regulation. Experiment 3 and Experiment 4 investigated the role of cognitive sets (divergent thinking) and motivational processes (thinking about one's action resources) in enhancing accommodation or assimilation. As expected, accommodation was triggered by an intervention activating divergent thought, and participants were more assimilative when they thought about their action resources. In sum, the results indicate that assimilation and accommodation can be induced experimentally; they were systematically dependent on physical, cognitive, and motivational states. The implications of the findings were discussed in the light of the dual-process model.

  13. [Difficult Diagnosis of Non-strabismic Binocular and Accommodative Disorders].

    PubMed

    Kříž, P

    2016-01-01

    The aim of the paper is to inform about the current approach to the diagnosis of non-strabismic binocular and accommodative disorders. A large number of studies quote high occurrence of them in both clinical and nonclinical populations. They also point out the presence of a diagnosis deficiency or insufficient diagnosis within the optometric and ophthalmological practice. The representation in population depends not only on race and age, but also on the methodology of diagnostic tests, and the chosen diagnostic criteria in particular. For the purpose of division of binocular and accommodative disorders there are several classification systems. The widespread classification facilitates a better understanding of a great spectrum of symptoms, assignment of the characteristic signs, and decision about appropriateness and choice of treatment types.Key words: heteroforie, binocular disorders, accommodative disorders, fusional vergence, convergence insufficiency, convergence excess.

  14. Magnitude of visual accommodation to a head-up display

    NASA Technical Reports Server (NTRS)

    Leitner, E. F.; Haines, R. F.

    1981-01-01

    The virtual image symbology of head-up displays (HUDs) is presented at optical infinity to the pilot. This design feature is intended to help pilots maintain visual focus distance at optical infinity. However, the accommodation response could be nearer than optical infinity, due to an individual's dark focus response. Accommodation responses were measured of two age groups of airline pilots to: (1) static symbology on a HUD; (2) a landing site background at optical infinity; (3) the combination of the HUD symbology and the landing site background; and (4) complete darkness. Results indicate that magnitude of accommodation to HUD symbology, with and without the background, is not significantly different from an infinity focus response for either age group. The dark focus response is significantly closer than optical infinity for the younger pilots, but not the older pilots, a finding consistent with previous research.

  15. Accommodative response and cortical activity during sustained attention.

    PubMed

    Poltavski, Dmitri V; Biberdorf, David; Petros, Thomas V

    2012-06-15

    Greater accommodative lag and vergence deficits have been linked to attentional deficits similar to those observed in Attention Deficit Hyperactivity Disorder (ADHD). The purpose of the present study was to assess the effect of accommodative-vergence stress on a measure of sustained attention (Conners CPT) used in the diagnosis of ADHD. Twenty-seven normal non-ADHD adults completed the Conners CPT twice: wearing -2.00 D lenses and normally (without the -2.00 D lenses) in a counterbalanced order with at least 24 h between the sessions. Simultaneous recording of participants' dynamic accommodative responses was performed from the right eye using the Grand Seiko WAM-5500 auto-refractor and electroencephalographic activity (EEG) in the left prefrontal region using the Neurosky Mindset headset. The results demonstrated a significantly greater accommodative lag in the -2.00 D stress condition and a significantly poorer performance on the Conners CPT as indexed by slower reaction time, greater standard error of hit reaction time, grater response variability, poorer stimulus detectability and a greater number of perseverations. No differences were observed on measures of EEG in the theta (4-7 Hz), alpha (8-12 Hz), and beta (12-20 Hz) bands. Moreover, when directly juxtaposed with each EEG band in multiple linear regression analyses, greater accommodative lag in the stress condition was significantly associated with a greater probability of clinical classification on the Conners CPT, and was also marginally predictive of the number of omissions recorded in the stress condition. The results demonstrated that sustained attention can be influenced by such factors as accommodative-vergence stress and suggest that bottom-up processes can contribute to and potentially exacerbate attentional problems in individuals with ADHD. The study also showed that cortical dysfunction (while sufficient) may not be a necessary condition for attentional deficits.

  16. Immunity-Based Accommodation of Aircraft Subsystem Failures

    NASA Astrophysics Data System (ADS)

    Togayev, Adil

    This thesis presents the design, development, and flight-simulation testing of an artificial immune system (AIS) based approach for accommodation of different aircraft subsystem failures. Failure accommodation is considered as part of a complex integrated AIS scheme that contains four major components: failure detection, identification, evaluation, and accommodation. The accommodation part consists of providing compensatory commands to the aircraft under specific abnormal conditions based on previous experience. In this research effort, the possibility of building an AIS allowing the extraction of pilot commands is investigated. The proposed approach is based on structuring the self (nominal conditions) and the non-self (abnormal conditions) within the AIS paradigm, as sets of artificial memory cells (mimicking behavior of T-cells, B-cells, and antibodies) consisting of measurement strings, over pre-defined time windows. Each string is a set of features values at each sample time of the flight including pilot inputs, system states, and other variables. The accommodation algorithm relies on identifying the memory cell that is the most similar to the in-coming measurements. Once the best match is found, control commands corresponding to this match will be extracted from the memory and used for control purposes. The proposed methodology is illustrated through simulation of simple maneuvers at nominal flight conditions, different actuators, and sensor failure conditions. Data for development and demonstration have been collected from West Virginia University 6-degrees-of-freedom motion-based flight simulator. The aircraft model used for this research represents a supersonic fighter which includes model following adaptive control laws based on non-linear dynamic inversion and artificial neural network augmentation. The simulation results demonstrate the possibility of extracting pilot compensatory commands from the self/non-self structure and the capability of the AIS

  17. Assessing fuel spill risks in polar waters: Temporal dynamics and behaviour of hydrocarbons from Antarctic diesel, marine gas oil and residual fuel oil.

    PubMed

    Brown, Kathryn E; King, Catherine K; Kotzakoulakis, Konstantinos; George, Simon C; Harrison, Peter L

    2016-09-15

    As part of risk assessment of fuel oil spills in Antarctic and subantarctic waters, this study describes partitioning of hydrocarbons from three fuels (Special Antarctic Blend diesel, SAB; marine gas oil, MGO; and intermediate grade fuel oil, IFO 180) into seawater at 0 and 5°C and subsequent depletion over 7days. Initial total hydrocarbon content (THC) of water accommodated fraction (WAF) in seawater was highest for SAB. Rates of THC loss and proportions in equivalent carbon number fractions differed between fuels and over time. THC was most persistent in IFO 180 WAFs and most rapidly depleted in MGO WAF, with depletion for SAB WAF strongly affected by temperature. Concentration and composition remained proportionate in dilution series over time. This study significantly enhances our understanding of fuel behaviour in Antarctic and subantarctic waters, enabling improved predictions for estimates of sensitivities of marine organisms to toxic contaminants from fuels in the region.

  18. Space Station accommodation engineering for Life Sciences Research Facilities

    NASA Technical Reports Server (NTRS)

    Hilchey, J.; Gustan, E.; Rudiger, C. E.

    1984-01-01

    Exploratory studies conducted by NASA Marshall Space Flight Center and several contractors in connection with defining the design requirements, parameters, and tradeoffs of the Life Sciences Research Facilities for nonhuman test subjects aboard the Space Station are reviewed. The major system discriminators which determine the size of the accommodation system are identified, along with a number of mission options. Moreover, characteristics of several vivarium concepts are summarized, focusing on the cost, size, variable-g capability, and the number of specimens accommodated. Finally, the objectives of the phase B studies of the Space Station Laboratory, which are planned for FY85, are described.

  19. Analysis on the accommodation of renewable energy in northeast China

    NASA Astrophysics Data System (ADS)

    Liu, Jun; Zhang, Jinfang; Tian, Feng; Mi, Zhe

    2017-01-01

    The accommodation and curtailment of renewable energy in northeast China have attracted much attention with the rapid growth of wind and solar power generation. Large amount of wind power has been curtailed or abandoned in northeast China due to several reasons, such as, the redundancy of power supplies, inadequate power demands, imperfect power structure with less flexibility and limited cross-regional transmission capacity. In this paper, we use multi-area production simulation to analyse the accommodation of renewable energy in northeast China by 2020. Furthermore, we suggest the measures that could be adopted in generation, grid and load side to reduce curtailment of renewables.

  20. Pupil movements to light and accommodative stimulation - A comparative study.

    NASA Technical Reports Server (NTRS)

    Semmlow, J.; Stark, L.

    1973-01-01

    Isolation and definition of specific response components in pupil reflexes through comparison of the dynamic features of light-induced and accommodation-induced pupil movements. A quantitative analysis of the behavior of the complex nonlinear pupil responses reveals the presence of two independent nonlinear characteristics: a range-dependent gain and a direction dependence or movement asymmetry. These nonlinear properties are attributed to motor processes because they are observable in pupil responses to both light and accommodation stimuli. The possible mechanisms and consequences of these pupil response characteristics are quantitatively defined and discussed.

  1. Adapting your teaching to accommodate the net generation of learners.

    PubMed

    Skiba, Diane J; Barton, Amy J

    2006-05-31

    Educators are faced with the challenge of adapting their teaching styles to accommodate a new generation of learners. The Net Generation or Millennials, who are now entering colleges and universities, have learning expectations, styles, and needs different from past students. This article assists educators in teaching the Net Generation by highlighting the characteristics of the Net Generation and providing examples of how to adapt teaching strategies to accommodate the Net Generation, in light of their preferences for digital literacy, experiential learning, interactivity, and immediacy.

  2. Against the accommodation of subjective healthcare provider beliefs in medicine: counteracting supporters of conscientious objector accommodation arguments.

    PubMed

    Smalling, Ricardo; Schuklenk, Udo

    2017-04-01

    We respond in this paper to various counter arguments advanced against our stance on conscientious objection accommodation. Contra Maclure and Dumont, we show that it is impossible to develop reliable tests for conscientious objectors' claims with regard to the reasonableness of the ideological basis of their convictions, and, indeed, with regard to whether they actually hold they views they claim to hold. We demonstrate furthermore that, within the Canadian legal context, the refusal to accommodate conscientious objectors would not constitute undue hardship for such objectors. We reject concerns that refusing to accommodate conscientious objectors would limit the equality of opportunity for budding professionals holding particular ideological positions. We also clarify various misrepresentations of our views by respondents Symons, Glick and Jotkowitz, and Lyus.

  3. Leading the Way to Appropriate Selection, Implementation, and Evaluation of the Read-Aloud Accommodation

    ERIC Educational Resources Information Center

    Thurlow, Martha L.; Lazarus, Sheryl S.; Hodgson, Jennifer R.

    2012-01-01

    The read-aloud accommodation is one of the most frequently used accommodations. Many educators need training to more confidently select, implement, and evaluate the use of the read-aloud accommodation. Planning by special education leaders can help ensure that test day goes smoothly for students who need the read-aloud accommodation.

  4. Success in baccalaureate nursing programs: a matter of accommodation?

    PubMed

    Haislett, J; Hughes, R B; Atkinson, G; Williams, C L

    1993-02-01

    This article explores student learning styles as an important variable in four-year baccalaureate nursing programs. Student learning styles were assessed by Kolb's Learning Style Inventory-1985 (LSI-1985), which identifies the accommodator, diverger, assimilator, and converger learning styles. The authors examined the relationship between learning style and academic performance as measured by grade-point ratio (GPR) and studied behaviors and attitudes as measured by Brown and Holtzman's (1964) Survey of Study Habits and Attitudes. Analysis indicated that this sample (N = 100) included mainly assimilators and divergers, making reflective observation the most common mode of learning. Compared to the accommodator/converger group, the assimilator/diverger group earned a significantly higher GPR, significantly better scores on the study habits variable of Work Methods (WM), and moderately better scores on the study attitude variable of Educational Acceptance (EA). Accommodators were identified as the most at-risk learning style group, and specific interventions were suggested to assist accommodators in adapting to the academic rigors of a nursing curriculum.

  5. The accommodative ciliary muscle function is preserved in older humans

    PubMed Central

    Tabernero, Juan; Chirre, Emmanuel; Hervella, Lucia; Prieto, Pedro; Artal, Pablo

    2016-01-01

    Presbyopia, the loss of the eye’s accommodation capability, affects all humans aged above 45–50 years old. The two main reasons for this to happen are a hardening of the crystalline lens and a reduction of the ciliary muscle functionality with age. While there seems to be at least some partial accommodating functionality of the ciliary muscle at early presbyopic ages, it is not yet clear whether the muscle is still active at more advanced ages. Previous techniques used to visualize the accommodation mechanism of the ciliary muscle are complicated to apply in the older subjects, as they typically require fixation stability during long measurement times and/or to have an ultrasound probe directly in contact with the eye. Instead, we used our own developed method based on high-speed recording of lens wobbling to study the ciliary muscle activity in a small group of pseudophakic subjects (around 80 years old). There was a significant activity of the muscle, clearly able to contract under binocular stimulation of accommodation. This supports a purely lenticular-based theory of presbyopia and it might stimulate the search for new solutions to presbyopia by making use of the remaining contraction force still presented in the aging eye. PMID:27151778

  6. View north of inside machine shop 36; shop floor accommodates ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View north of inside machine shop 36; shop floor accommodates lathes capable of machining a cylinder 60 inches in diameter and 75 feet long; other equipment includes horizontal and vertical jig borders, hydraulic tube straighteners and other equipment for precision machining of large ship components. - Naval Base Philadelphia-Philadelphia Naval Shipyard, Structure Shop, League Island, Philadelphia, Philadelphia County, PA

  7. Student Accommodation Projects: A Guide to PFI Contracts. Good Practice.

    ERIC Educational Resources Information Center

    Curtis, Pinsent

    This guide is intended for higher education institutions in England that are about to embark on student residential accommodation projects. It focuses on procurements under the Private Financial Initiative (PFI), a form of Public Private Partnership in the United Kingdom, but other approaches are considered. The guide draws on good practices from…

  8. The accommodative ciliary muscle function is preserved in older humans

    NASA Astrophysics Data System (ADS)

    Tabernero, Juan; Chirre, Emmanuel; Hervella, Lucia; Prieto, Pedro; Artal, Pablo

    2016-05-01

    Presbyopia, the loss of the eye’s accommodation capability, affects all humans aged above 45–50 years old. The two main reasons for this to happen are a hardening of the crystalline lens and a reduction of the ciliary muscle functionality with age. While there seems to be at least some partial accommodating functionality of the ciliary muscle at early presbyopic ages, it is not yet clear whether the muscle is still active at more advanced ages. Previous techniques used to visualize the accommodation mechanism of the ciliary muscle are complicated to apply in the older subjects, as they typically require fixation stability during long measurement times and/or to have an ultrasound probe directly in contact with the eye. Instead, we used our own developed method based on high-speed recording of lens wobbling to study the ciliary muscle activity in a small group of pseudophakic subjects (around 80 years old). There was a significant activity of the muscle, clearly able to contract under binocular stimulation of accommodation. This supports a purely lenticular-based theory of presbyopia and it might stimulate the search for new solutions to presbyopia by making use of the remaining contraction force still presented in the aging eye.

  9. Accommodating Students' Sensory Learning Modalities in Online Formats

    ERIC Educational Resources Information Center

    Allison, Barbara N.; Rehm, Marsha L.

    2016-01-01

    Online classes have become a popular and viable method of educating students in both K-12 settings and higher education, including in family and consumer sciences (FCS) programs. Online learning dramatically affects the way students learn. This article addresses how online learning can accommodate the sensory learning modalities (sight, hearing,…

  10. Mechanically implementable accommodation matrices for passive force control

    SciTech Connect

    Goswami, A.; Peshkin, M.

    1999-08-01

    Robot force control implemented by means of passive mechanical devices has inherent advantages over active implementations with regard to stability, response rapidity, and physical robustness. The class of devices considered in this paper consists of a Stewart platform-type mechanism interconnected with a network of adjustable mechanical elements such as springs and dampers. The control law repertoire of such a device, imagined as a robot wrist, is given by the range of admittance matrices that it may be programmed to possess. This paper focuses on wrists incorporating damper networks for which the admittance matrices reduce to accommodation or inverse-damping matrices. The authors show that a hydraulic network of fully adjustable damper elements may attain any diagonally dominant accommodation matrix. They describe the technique of selecting the individual damping coefficients to design a desired matrix. They identify the set of dominant matrices as a polyhedral convex cone in the space of matrix entries, and show that each dominant matrix can be composed of a positive linear combination of a fixed set of basis matrices. The overall wrist-accommodation matrix is obtained by projecting the accommodation matrix of the damper network through the wrist kinematics. The linear combination of the dominant basis matrices projected through the wrist kinematics generates the entire space of mechanically implementable force-control laws. The authors quantify the versatility of mechanically implementable force-control laws by comparing this space to the space of all matrices.

  11. The Effect of Intensified Language Exposure on Accommodating Talker Variability

    ERIC Educational Resources Information Center

    Antoniou, Mark; Wong, Patrick C. M.; Wang, Suiping

    2015-01-01

    Purpose: This study systematically examined the role of intensified exposure to a second language on accommodating talker variability. Method: English native listeners (n = 37) were compared with Mandarin listeners who had either lived in the United States for an extended period of time (n = 33) or had lived only in China (n = 44). Listeners…

  12. Understanding and Accommodating Students with Depression in the Classroom

    ERIC Educational Resources Information Center

    Crundwell, R. Marc; Killu, Kim

    2007-01-01

    Depression and mood disorders present a significant challenge in the classroom; resulting symptoms can impact memory, recall, motivation, problem solving, task completion, physical and motor skills, and social interactions. Little information is available on practical instructional accommodations and modifications for use by the classroom teacher.…

  13. Manifold gasket accommodating differential movement of fuel cell stack

    DOEpatents

    Kelley, Dana A.; Farooque, Mohammad

    2007-11-13

    A gasket for use in a fuel cell system having at least one externally manifolded fuel cell stack, for sealing the manifold edge and the stack face. In accordance with the present invention, the gasket accommodates differential movement between the stack and manifold by promoting slippage at interfaces between the gasket and the dielectric and between the gasket and the stack face.

  14. Disability Accommodations in Online Courses: The Graduate Student Experience

    ERIC Educational Resources Information Center

    Terras, Katherine; Leggio, Joseph; Phillips, Amy

    2015-01-01

    Research is beginning to demonstrate that online learning may afford students with disabilities enhanced opportunities for academic success. In this study, the authors interviewed 11 graduate students to determine their experiences with disability accommodations in online courses and their perceptions of the relationship between those…

  15. Promoting Independence for Wheelchair Users: The Role of Home Accommodations

    ERIC Educational Resources Information Center

    Allen, Susan; Resnik, Linda; Roy, Jason

    2006-01-01

    Purpose: The objective of this research is to investigate whether home accommodations influence the amount of human help provided to a nationally representative sample of adults who use wheelchairs. Design and Methods: We analyzed data from the Adult Disability Follow-back Survey (DFS), Phase II, of the Disability Supplement to the 1994-1995…

  16. Accommodating College Students with Learning Disabilities: ADD, ADHD, and Dyslexia

    ERIC Educational Resources Information Center

    Vickers, Melana Zyla

    2010-01-01

    Universities are providing extra time on tests, quiet exam rooms, in-class note-takers, and other assistance to college students with modest learning disabilities. But these policies are shrouded in secrecy. This paper, "Accommodating College Students with Learning Disabilities: ADD, ADHD, and Dyslexia," by Melana Zyla Vickers, examines…

  17. Language Shift and Language Accommodation across Family Generations in Taiwan

    ERIC Educational Resources Information Center

    Sandel, Todd L.; Chao, Wen-Yu; Liang, Chung-Hui

    2006-01-01

    This study explored language shift and accommodation among bilingual Mandarin and Tai-gi (also called Hokkien, Holo, Tai-gu, Taiwan Min, Taiwanese) families in Taiwan. From the 1940s until the 1980s the Chinese Nationalist Party (KMT) on Taiwan promoted Mandarin Chinese. Recent years have witnessed a shift in policy: since 2001 elementary schools…

  18. Coworker Informal Work Accommodations to Family: Scale Development and Validation

    ERIC Educational Resources Information Center

    Mesmer-Magnus, Jessica; Murase, Toshio; DeChurch, Leslie A.; Jimenez, Miliani

    2010-01-01

    Drawing on research regarding the utility of coworker support in mitigating work/family conflict, the authors developed a scale to measure Coworker-enacted Informal Work Accommodations to Family (C-IWAF). C-IWAF differs from coworker support in that it describes actual behaviors coworkers engage in to help one another deal with incompatible work…

  19. View of button board which accommodates the 1200 button switches ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of button board which accommodates the 1200 button switches which manually control the indicating pilot lights on the model board. Not all switches are active, some switches were installed for a planned, but never constructed, system expansion. - Thirtieth Street Station, Load Dispatch Center, Thirtieth & Market Streets, Railroad Station, Amtrak (formerly Pennsylvania Railroad Station), Philadelphia, Philadelphia County, PA

  20. 13 CFR 112.6 - Discrimination in accommodations or services.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Discrimination in accommodations or services. 112.6 Section 112.6 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NONDISCRIMINATION IN FEDERALLY ASSISTED PROGRAMS OF SBA-EFFECTUATION OF TITLE VI OF THE CIVIL RIGHTS ACT OF...

  1. Accommodating Students with Disabilities: A Practical Guide for the Faculty.

    ERIC Educational Resources Information Center

    Chang, Moon K.; And Others

    This guide is designed to assist university professors in making decisions on their instructional planning and delivery by expanding and refining their repertoire of accommodations for their students with visual and hearing impairments, physical disabilities, and learning disabilities. Part 1 suggests a five-step procedure for making decisions on…

  2. Accommodating Students with Disabilities: A Guide for School Teachers.

    ERIC Educational Resources Information Center

    Chang, Moon K.; And Others

    This guide is designed as a resource to assist teachers in making decisions on their instructional planning and delivery by expanding and refining their repertoire of ways and means of making accommodations for students with learning disabilities and visual, hearing, and physical impairments. Part 1 presents ways of providing accommodations…

  3. Pharmacologically Stimulated Pupil and Accommodative Changes in Guinea Pigs

    PubMed Central

    Ostrin, Lisa A.; Garcia, Mariana B.; Choh, Vivian; Wildsoet, Christine F.

    2014-01-01

    Purpose. The guinea pig is being used increasingly as a model of human myopia. As accommodation may influence the effects of manipulations used in experimental myopia models, understanding the accommodative ability of guinea pigs is important. Here, nonselective muscarinic agonists were used as pharmacological tools to study guinea pig accommodation. Methods. Measurements were made on 15 pigmented guinea pigs. For in vivo testing, animals were anesthetized and, following baseline measurements, 2% pilocarpine was applied topically. Measurements included A-scan ultrasonography, optical coherence tomography (OCT) imaging, corneal topography, and refraction. In vitro lens scanning experiments were performed using anterior segment preparations, with measurements before and during exposure to carbachol. Anterior segment structures were examined histologically and immunohistochemistry was done to characterize the muscarinic receptor subtypes present. Results. In vivo, pilocarpine induced a myopic shift in refractive error coupled to a small, but consistent decrease in anterior chamber depth (ACD), a smaller and more variable increase in lens thickness, and a decrease in pupil size. Lens thickness increases were short-lived (10 minutes), while ACD and pupil size decreased over 20 minutes. Corneal curvature was not significantly affected. Carbachol tested on anterior segment preparations in vitro was without effect on lens back vertex distance, but did stimulate pupil constriction. Immunohistochemistry indicated the presence of muscarinic receptor subtypes 1 to 5 in the iris and ciliary body. Conclusions. The observed pilocarpine-induced changes in ACD, lens thickness, and refraction are consistent with active accommodation in the guinea pig, through cholinergic muscarinic stimulation. PMID:25097245

  4. Science Language Accommodation in Elementary School Read-Alouds

    ERIC Educational Resources Information Center

    Glass, Rory; Oliveira, Alandeom W.

    2014-01-01

    This study examines the pedagogical functions of accommodation (i.e. provision of simplified science speech) in science read-aloud sessions facilitated by five elementary teachers. We conceive of read-alouds as communicative events wherein teachers, faced with the task of orally delivering a science text of relatively high linguistic complexity,…

  5. Accommodating the Spectrum of Individual Abilities. Clearinghouse Publication 81.

    ERIC Educational Resources Information Center

    Commission on Civil Rights, Washington, DC.

    The monograph addresses legal issues involving discrimination against handicapped persons and the key legal requirement of reasonable accommodation. Four chapters in Part I examine background issues, including definitions and statistical overviews of handicaps; historical attitudes toward handicapped persons and an analysis of the extent of…

  6. Accommodating the Special Learner in Secondary General Music Classes

    ERIC Educational Resources Information Center

    VanWeelden, Kimberly

    2011-01-01

    It can be challenging to know which accommodations for special learners can be used within the various secondary general music class settings. Fortunately, there have been several recent music education and therapy articles based on special education practices that have addressed techniques for working with students with special needs in music.…

  7. Analysis and applications of accommodative lenses for vision corrections

    NASA Astrophysics Data System (ADS)

    Lin, Jui-Teng; Jiang, Minshan; Chang, Chun-Lin; Hong, Yu-Ling; Ren, Qiushi

    2011-01-01

    Analysis and applications of vision correction via accommodating intraocular lens (AIOL) are presented. By Gaussian optics, analytic formulas for the accommodation rate function (M) for two-optics and three-optics systems are derived and compared with the exact numerical results. In a single-optics AIOL, typical value of M is (0.5-1.5) D/mm, for an IOL power of (10-20) diopter. For a given IOL power, higher M is achieved in positive-IOL than negative-IOL. In the dual-optics AIOL, maximum accommodation is predicted when the front positive-optics moves toward the corneal plan and the back negative-optics moves backward. Our analytic formulas predict that greater accommodative rate may be achieved by using a positive-powered front optics, a general feature when either front or back optics is mobile. The M function is used to find the piggy-back IOL power for customized design based on the individual ocular parameters. Many of the new features demonstrated in this study can be easily realized by our analytic formulas, but not by raytracing method.

  8. The Nonverbal Accommodation Analysis System (NAAS): Initial application and evaluation

    PubMed Central

    D'Agostino, Thomas A.; Bylund, Carma L.

    2016-01-01

    Objective To describe the development, initial application, and evaluation of the Nonverbal Accommodation Analysis System (NAAS). Grounded in Communication Accommodation Theory, this coding system provides a method for analyzing physician and patient nonverbal accommodation behaviors within medical consultations. Methods Video recordings of 45 new visit consultations at a comprehensive cancer center were coded using the NAAS. Inter-rater and intra-rater reliability were assessed. For validation purposes, two independent coders rated all consultations for theoretically-related constructs. Results The NAAS demonstrated high levels of reliability. Statistically significant correlations were observed across all 10 behavior categories for both inter-rater and intra-rater reliability. Evidence of content and construct validity was also observed. Conclusion The current study presents the initial application and evaluation of a coding system meant for analysis of the nonverbal behavior of physicians and patients within medical consultations. The results of this initial trial and psychometric evaluation provide evidence of the NAAS as a valid and reliable nonverbal accommodation coding system. PMID:20851559

  9. Maximising Occupancy. Accommodation Management Module. Operational Management Programme.

    ERIC Educational Resources Information Center

    Holland, Julia

    This module on maximizing occupancy is intended to cover the basic framework for analysis of occupancy in accommodations and for identification of ways to improve it on both a long-term and day-to-day basis. The material is presented in a self-instructional format in seven sections. At the beginning of each section is a statement of the objectives…

  10. Accommodation, Cafes and Restaurants. Industry Training Monograph No. 8.

    ERIC Educational Resources Information Center

    Dumbrell, Tom

    Australia's accommodation, cafes, and restaurants industry represents more than half of the nation's total tourism and hospitality employment. It accounts for roughly 4.5% of all jobs in Australia (400,000 workers). Since 1987, the number of jobs in the sector has risen from about 257,000 to about 372,000. Approximately 57% of employees are…

  11. Accommodations in Homeschool Settings for Children with Special Education Needs

    ERIC Educational Resources Information Center

    Stoudt, Patricia Koelsch

    2012-01-01

    This qualitative study was designed to examine how homeschooling parents in Pennsylvania make the determination to engage with public school districts to accommodate the special education needs (SEN) of their children. This phenomenological study used direct interviews with 30 Pennsylvania families who are homeschooling children with SEN. Data…

  12. 13 CFR 112.6 - Discrimination in accommodations or services.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Discrimination in accommodations or services. 112.6 Section 112.6 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NONDISCRIMINATION IN FEDERALLY ASSISTED PROGRAMS OF SBA-EFFECTUATION OF TITLE VI OF THE CIVIL RIGHTS ACT OF...

  13. Sequential strategies of accommodation: a new method in courtroom.

    PubMed

    Gnisci, Augusto

    2005-12-01

    The aim of this research is to show the fruitfulness of a sequential analysis approach applied to the study of the dynamic and sequential character of accommodation strategies proposed by communication accommodation theory (CAT). It was applied to the convergence, divergence, maintenance, and control strategies undertaken in the courtroom during 47 hostile examinations from a single criminal case. Each of the 1,850 question-answer exchanges was classified for the content aspects (types of questions and of answers) and for the modalities of turn taking (interrupting, latching, and pausing) by both lawyer and witness. Log-linear analyses were performed. The main results show that the lawyer and the witness used both accommodation and maintenance strategies, even if the latter are used more by the lawyer. Both participants made use of unimodal and bimodal strategies and tended to reciprocate the behaviour of the interlocutor. The conclusions emphasize the advantage of using the sequential analysis approach to the accommodation strategies of CAT: studying multiple behaviours, identifying the direction and the modalities of the strategies even if they act simultaneously, and providing a means to evaluate the weight of the strategies on the whole and in detail.

  14. Development of the Reasonable Accommodation Factor Survey: Results and Implications

    ERIC Educational Resources Information Center

    Dong, Shengli; MacDonald-Wilson, Kim L.; Fabian, Ellen

    2010-01-01

    The purpose of this study was (a) to explore the latent factors in the "Reasonable Accommodation Factor Survey" (RAFS) instrument and (b) to compare scores on the latent factors of the RAFS by participant's role. Eight latent factors were identified through an exploratory factor analysis with orthogonal rotation. The reliability tests…

  15. 23 CFR 645.211 - State transportation department accommodation policies.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false State transportation department accommodation policies... highway and utility construction and maintenance. (c) The State transportation department's standards for... highway design standards or other measures which the State transportation department deems necessary...

  16. 23 CFR 645.211 - State transportation department accommodation policies.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false State transportation department accommodation policies... highway and utility construction and maintenance. (c) The State transportation department's standards for... highway design standards or other measures which the State transportation department deems necessary...

  17. 23 CFR 645.211 - State transportation department accommodation policies.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false State transportation department accommodation policies... highway and utility construction and maintenance. (c) The State transportation department's standards for... highway design standards or other measures which the State transportation department deems necessary...

  18. 23 CFR 645.211 - State transportation department accommodation policies.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false State transportation department accommodation policies... highway and utility construction and maintenance. (c) The State transportation department's standards for... highway design standards or other measures which the State transportation department deems necessary...

  19. Accommodating Students with Disabilities in Soil Science Activities

    ERIC Educational Resources Information Center

    Langley-Turnbaugh, S. J.; Murphy, Kate; Levin, E.

    2004-01-01

    Soil science education is lacking in terms of accommodations for persons with disabilities. Individuals with disabilities are often excluded from soil science activities in school, and from soil science careers. GLOBE (Global Learning Observations to Benefit the Environment) is a worldwide, hands-on primary and secondary school-based education and…

  20. The Contribution of University Accommodation to International Student Security

    ERIC Educational Resources Information Center

    Paltridge, Toby; Mayson, Susan; Schapper, Jan

    2010-01-01

    In this paper we argue that living in university accommodation is a possible means of improving the security of international students. Our argument is supported by a qualitative case study of a single Hall of Residence on Monash University's Clayton campus. Data were collected primarily from interviews with three groups of participants--six…

  1. 23 CFR 645.211 - State transportation department accommodation policies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Guide for Accommodating Utilities Within Highway Right-of-Way” and “Roadside Design Guide” to assist in... highway and utility construction and maintenance. (c) The State transportation department's standards for... highway design standards or other measures which the State transportation department deems necessary...

  2. Translation Accommodations Framework for Testing English Language Learners in Mathematics

    ERIC Educational Resources Information Center

    Solano-Flores, Guillermo

    2012-01-01

    The present framework is developed under contract with the Smarter Balanced Assessment Consortium (SBAC) as a conceptual and methodological tool for guiding the reasonings and actions of contractors in charge of developing and providing test translation accommodations for English language learners. The framework addresses important challenges in…

  3. Teaching Disability Employment Discrimination Law: Accommodating Physical and Mental Disabilities

    ERIC Educational Resources Information Center

    Kulow, Marianne DelPo

    2012-01-01

    Disability employment discrimination is often treated summarily in legal environment courses. This is actually a topic with significant practical application in the workplace since managers are often those who are confronted with accommodation requests. It is therefore desirable to include a class with hands-on exercises for students to begin to…

  4. The alteration of profile analysis to accommodate testing functions

    NASA Technical Reports Server (NTRS)

    Myers, R. H.

    1979-01-01

    The development of a methodology was studied for testing differences among several pilot functions, where the data points represent averages at various frequencies. Topics discussed include: basic assumptions, hypothesis, profile analysis, alteration of profile analysis to accommodate testing functions, test and procedures, and power of tests.

  5. Speech Accommodation without Priming: The Case of Pitch

    ERIC Educational Resources Information Center

    Gijssels, Tom; Casasanto, Laura Staum; Jasmin, Kyle; Hagoort, Peter; Casasanto, Daniel

    2016-01-01

    People often accommodate to each other's speech by aligning their linguistic production with their partner's. According to an influential theory, the Interactive Alignment Model, alignment is the result of priming. When people perceive an utterance, the corresponding linguistic representations are primed and become easier to produce. Here we…

  6. Treatment or Accommodation for Adults with Challenging Behaviors.

    ERIC Educational Resources Information Center

    Fox, Robert A.

    2003-01-01

    This article discusses the difficulties some individuals with developmental disabilities have in responding to behavioral treatment and suggests an alternative to traditional behavioral treatment programs. It describes the use of the accommodation approach in which an individual's environment is rearranged to prevent or lower the occurrence of…

  7. 46 CFR 127.260 - Ventilation for accommodations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Ventilation for accommodations. 127.260 Section 127.260 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS CONSTRUCTION AND... cognizant OCMI is satisfied that a natural system, such as opening windows, portholes, or doors,...

  8. Design Criteria for Public School Plants Accommodating the Physically Disabled.

    ERIC Educational Resources Information Center

    Missouri State Dept. of Education, Jefferson City. School Building Services.

    Criteria are presented for implementation in the design and construction of school buildings and facilities so as to obviate hazards to individuals with physical disabilities. Accommodations for the physically handicapped are considered for the following--(1) public walks, (2) parking lots, (3) ramps with gradients and handrails, (4) entrances,…

  9. Accommodations Manual: How to Select, Administer, and Evaluate Use of Accommodations for Instruction and Assessment of Students with Disabilities. Third Edition

    ERIC Educational Resources Information Center

    Christensen, L.; Carver, W.; VanDeZande, J.; Lazarus, S.

    2011-01-01

    The Council of Chief State School Officers' "Accommodations Manual: How to Select, Administer, and Evaluate the Use of Accommodations for Instruction and Assessment of Students with Disabilities" was first developed to establish guidelines for states to use for the selection, administration, and evaluation of accommodations for…

  10. Accommodating environmental variation in population models: metaphysiological biomass loss accounting.

    PubMed

    Owen-Smith, Norman

    2011-07-01

    1. There is a pressing need for population models that can reliably predict responses to changing environmental conditions and diagnose the causes of variation in abundance in space as well as through time. In this 'how to' article, it is outlined how standard population models can be modified to accommodate environmental variation in a heuristically conducive way. This approach is based on metaphysiological modelling concepts linking populations within food web contexts and underlying behaviour governing resource selection. Using population biomass as the currency, population changes can be considered at fine temporal scales taking into account seasonal variation. Density feedbacks are generated through the seasonal depression of resources even in the absence of interference competition. 2. Examples described include (i) metaphysiological modifications of Lotka-Volterra equations for coupled consumer-resource dynamics, accommodating seasonal variation in resource quality as well as availability, resource-dependent mortality and additive predation, (ii) spatial variation in habitat suitability evident from the population abundance attained, taking into account resource heterogeneity and consumer choice using empirical data, (iii) accommodating population structure through the variable sensitivity of life-history stages to resource deficiencies, affecting susceptibility to oscillatory dynamics and (iv) expansion of density-dependent equations to accommodate various biomass losses reducing population growth rate below its potential, including reductions in reproductive outputs. Supporting computational code and parameter values are provided. 3. The essential features of metaphysiological population models include (i) the biomass currency enabling within-year dynamics to be represented appropriately, (ii) distinguishing various processes reducing population growth below its potential, (iii) structural consistency in the representation of interacting populations and

  11. Resveratrol and curcumin synergistically induces apoptosis in cigarette smoke condensate transformed breast epithelial cells through a p21(Waf1/Cip1) mediated inhibition of Hh-Gli signaling.

    PubMed

    Mohapatra, Purusottam; Satapathy, Shakti Ranjan; Siddharth, Sumit; Das, Dipon; Nayak, Anmada; Kundu, Chanakya Nath

    2015-09-01

    Combination therapy using two or more small molecule inhibitors of aberrant signaling cascade in aggressive breast cancers is a promising therapeutic strategy over traditional monotherapeutic approaches. Here, we have studied the synergistic mechanism of resveratrol and curcumin induced apoptosis using in vitro (cigarette smoke condensate mediated transformed breast epithelial cell, MCF-10A-Tr) and in vivo (tumor xenograft mice) model system. Resveratrol exposure increased the intracellular uptake of curcumin in a dose dependent manner and caused apoptosis in MCF-10A-Tr cells. Approximately, ten fold lower IC50 value was noted in cells treated with the combination of resveratrol (3μM) and curcumin (3μM) in comparison to 30μM of resveratrol or curcumin alone. Resveratrol+curcumin combination caused apoptosis by increasing Bax/Bcl-xL ratio, Cytochrome C release, cleaved product of PARP and caspase 3 in cells. Interestingly, this combination unaltered the protein expressions of WNT-TCF and Notch signaling components, β-catenin and cleaved notch-1 val1744, respectively. Furthermore, the combination also significantly decreased the intermediates of Hedgehog-Gli cascade including SMO, SHH, Gli-1, c-MYC, Cyclin-D1, etc. and increased the level of p21(Waf/Cip1) in vitro and in vivo. A significant reduction of Gli- promoter activity was noted in combinational drug treated cells in comparison to individual drug treatment. Un-alteration of the expressions of the above proteins and Gli1 promoter activity in p21(Waf/Cip1) knockout cells suggests this combination caused apoptosis through p21(Waf/Cip1). Thus, our findings revealed resveratrol and curcumin synergistically caused apoptosis in cigarette smoke induced breast cancer cells through p2(Waf/Cip1) mediated inhibition of Hedgehog-Gli cascade.

  12. Pharmacodynamic study of the 7,8-dihydroxy-4-methylcoumarin-induced selective cytotoxicity toward U-937 leukemic cells versus mature monocytes: cytoplasmic p21(Cip1/WAF1) as resistance factor.

    PubMed

    Vázquez, Ramiro; Riveiro, María Eugenia; Mondillo, Carolina; Perazzo, Juan Carlos; Vermeulen, Mónica; Baldi, Alberto; Davio, Carlos; Shayo, Carina

    2013-07-15

    The development of tumor-selective drugs with low systemic toxicity has always been a major challenge in cancer treatment. Our group previously identified the 7,8-dihydroxy-4-methylcoumarin (DHMC) as a potential chemotherapeutic agent due to its potent, selective anti-proliferative and apoptosis-inducing effects on several cancer cell lines over peripheral blood mononuclear cells. However, there are still no published reports that can explain such selectivity of action. Herein, we addressed this question by using the U-937 promonocytic leukemia cell line, which can be forced to differentiate into a monocyte-like phenotype in vitro. U-937 cells differentiation is dependent on the nuclear expression of p21(Cip1/WAF1), a protein that is absent in immature U-937 cells but present in both the nucleus and the cytoplasm of normal DHMC-resistant monocytes. Considering that induction of differentiation rendered U-937 cells resistant to DHMC, we evaluated the possible causal role of cytoplasmic p21(Cip1/WAF1) in the onset of such resistance by employing U-937 cells stably transfected with a ZnCl2-inducible p21(Cip1/WAF1) variant lacking the nuclear localization signal (U-937/CB6-ΔNLS-p21 cells). Expression of cytoplasmic p21(Cip1/WAF1) did not induce differentiation of the cells but turned them resistant to DHMC through inhibition of JNK, a crucial mediator of DHMC-induced apoptosis in U-937 cells. Sub-acute toxicity evaluation of DHMC in Balb/c mice indicated that DHMC administered intraperitoneally at doses up to 100mg/kg induced no systemic damage. Collectively, our results explain for the first time the selective cytotoxicity of DHMC for tumor cells over normal monocytes, and encourage further in vivo studies on this compound as potential anti-leukemic agent.

  13. Correlation Among Six Biologic Factors (p53, p21{sup WAF1}, MIB-1, EGFR, HER2, and Bcl-2) and Clinical Outcomes After Curative Chemoradiation Therapy in Squamous Cell Cervical Cancer

    SciTech Connect

    Yamashita, Hideomi; Murakami, Naoya; Asari, Takao; Okuma, Kae; Ohtomo, Kuni; Nakagawa, Keiichi

    2009-07-15

    Purpose: The expressions of six cell-cycle-associated proteins were analyzed in cervical squamous cell carcinomas in correlation in a search for prognostic correlations in tumors treated with concurrent chemoradiation therapy (cCRT). Methods and Materials: The expressions of p53, p21/waf1/cip1, molecular immunology borstel-1 (MIB-1), epidermal growth factor receptor (EGFR), human epidermal growth factor receptor type 2 (HER2), and Bcl-2 were studied using an immunohistochemical method in 57 cases of cervical squamous cell carcinoma treated with cCRT. Patients received cCRT between 1998 and 2005. The mean patient age was 61 years (range, 27-82 years). The number of patients with Stage II, III, and IVA disease was 18, 29, and 10, respectively. Results: The number of patients with tumors positive for p53, p21/waf1/cip1, MIB-1, EGFR, HER2, and Bcl-2 was 26, 24, 49, 26, 13, and 11, respectively; no significant correlation was noted. The 5-year overall survival rates of HER2-positive and -negative patients was 76% vs. 44%, which was of borderline significance (p = 0.0675). No significant correlation was noted between overall survival and expressions of p53, p21/waf1/cip1, MIB-1, EGFR, and Bcl-2. No correlation was observed between local control and expression of any of the proteins. Conclusion: Expression of HER2 protein had a weak impact of borderline significance on overall survival in squamous cell carcinoma of the uterine cervix treated with cCRT. However, no clinical associations could be established for p53, p21/waf1/cip1, MIB-1, EGFR, and Bcl-2 protein expressions.

  14. Fractional vector calculus and fractional Maxwell's equations

    SciTech Connect

    Tarasov, Vasily E.

    2008-11-15

    The theory of derivatives and integrals of non-integer order goes back to Leibniz, Liouville, Grunwald, Letnikov and Riemann. The history of fractional vector calculus (FVC) has only 10 years. The main approaches to formulate a FVC, which are used in the physics during the past few years, will be briefly described in this paper. We solve some problems of consistent formulations of FVC by using a fractional generalization of the Fundamental Theorem of Calculus. We define the differential and integral vector operations. The fractional Green's, Stokes' and Gauss's theorems are formulated. The proofs of these theorems are realized for simplest regions. A fractional generalization of exterior differential calculus of differential forms is discussed. Fractional nonlocal Maxwell's equations and the corresponding fractional wave equations are considered.

  15. An extended soft-cube model for the thermal accommodation of gas atoms on solid surfaces

    NASA Astrophysics Data System (ADS)

    Burke, J. R.; Hollenbach, D. J.

    1980-01-01

    A numerical soft cube model was developed for calculating thermal accommodation coefficients alpha and trapping fractions f sub t for the interaction of gases incident upon solid surfaces. A semiempirical correction factor c which allows the calculation of alpha and f sub t when the collision times are long compared to the surface oscillator period were introduced. The processes of trapping, evaporation, and detailed balancing were discussed. The numerical method was designed to treat economically and with moderate (+ or - 20 percent) accuracy the dependence of alpha and f sub t on finite and different surface and gas temperatures for a large number of gas/surface combinations. Comparison was made with experiments of rare gases on tungsten and on alkalis, as well as one astrophysical case of H2 on graphite. The dependence of alpha on the soft cube dimensionless parameters is presented graphically.

  16. Down-regulation of dihydrofolate reductase inhibits the growth of endothelial EA.hy926 cell through induction of G1 cell cycle arrest via up-regulating p53 and p21(waf1/cip1) expression.

    PubMed

    Fei, Zhewei; Gao, Yong; Qiu, Mingke; Qi, Xianqin; Dai, Yuxin; Wang, Shuqing; Quan, Zhiwei; Liu, Yingbin; Ou, Jingmin

    2016-03-01

    Folic acid supplementation may meliorate cardiovascular disease risk by improving vascular endothelial structure and function. However, the underlying mechanisms are still lack of a global understanding. To be used, folic acid must be converted to 7,8-dihydrofolate by dihydrofolate reductase to generate one-carbon derivatives serving as important cellular cofactors in the synthesis of nucleotides and amino acids required for cell growth. Therefore, this study explored the effect of dihydrofolate reductase knockdown on endothelial EA.hy926 cell growth and the mechanism involved. We found that down-regulation of dihydrofolate reductase inhibited EA.hy926 cell proliferation, and induced G1 phase arrest. Meanwhile, the expression of regulators necessary for G1/S phase transition, such as cyclin-dependent kinases CDK2, CDK4 and CDK6, were remarkably down-regulated; by contrast, the cell cycle inhibitors p21(waf/cip1), p27(Kip1) and p53 were significantly up-regulated after dihydrofolate reductase knockdown. Furthermore, supplementation of 5-methyltetrahydrofolate to the dihydrofolate reductase knockdown cells could weaken the inhibitory effect of dihydrofolate reductase knockdown on cell proliferation, simultaneously, inducing the expression of p53 and p21(waf/cip1) falling back moderately. Our findings suggest that attenuating dihydrofolate reductase may cause imbalanced expression of cell cycle regulators, especially up-regulation of p53-p21(waf/cip1) pathway, leading to G1 cell cycle arrest, thereby inhibiting the growth of endothelial EA.hy926 cells.

  17. EPO gene expression promotes proliferation, migration and invasion via the p38MAPK/AP-1/MMP-9 pathway by p21WAF1 expression in vascular smooth muscle cells.

    PubMed

    Park, Sung Lyea; Won, Se Yeon; Song, Jun-Hui; Kambe, Taiho; Nagao, Masaya; Kim, Wun-Jae; Moon, Sung-Kwon

    2015-03-01

    The use of recombinant human erythropoietin (rHuEpo) can lead to hypertrophy and hyperplasia, and has induced the proliferation of vascular smooth muscle cells (VSMCs). The effect of the EPO gene in the migration and invasion of VSMCs remains unclear. In this study, overexpression of the EPO gene increased the DNA synthesis and phosphorylation of ERK1/2 and p38MAPK in VSMCs. In addition, EPO gene expression induced the migration and invasion of VSMCs via the expression of MMP-9 by the activation of NF-κB and AP-1 binding. A blockade of p38MAPK by specific p38MAPK inhibitor SB203580 led to a suppression of the increased DNA synthesis, migration, and invasion of VSMCs that was induced by the EPO gene. SB203580 treatment blocked the increased expression of MMP-9 through the binding activity of AP-1. Transfection of the EPO gene with VSMCs was associated with the up-regulation of cyclin D1/CDK4, cyclin E/CDK2, and p21WAF1, and with the down-regulation of p27KIP1. The specific suppression of p21WAF1 expression by siRNA rescued the enhancement of DNA synthesis via the phosphorylation of p38MAPK and the increase in migration and invasion through AP-1-mediated MMP-9 expression in EPO gene transfectants. These novel findings demonstrate that p21WAF1 regulates the proliferation, migration and invasion of VSMC induced by EPO gene.

  18. Cytoplasmic p21(CIP1/WAF1), ERK1/2 activation, and cytoskeletal remodeling are associated with the senescence-like phenotype after airborne particulate matter (PM(10)) exposure in lung cells.

    PubMed

    Sánchez-Pérez, Yesennia; Chirino, Yolanda I; Osornio-Vargas, Álvaro Román; Herrera, Luis A; Morales-Bárcenas, Rocío; López-Saavedra, Alejandro; González-Ramírez, Imelda; Miranda, Javier; García-Cuellar, Claudia María

    2014-02-10

    The exposure to particulate matter with a mean aerodynamic diameter ≤10 μm (PM10) from urban zones is considered to be a risk factor in the development of cancer. The aim of this work was to determine if PM10 exposure induces factors related to the acquisition of a neoplastic phenotype, such as cytoskeletal remodeling, changes in the subcellular localization of p21(CIP1/WAF1), an increase in β-galactosidase activity and changes in cell cycle. To test our hypothesis, PM10 from an industrial zone (IZ) and a commercial zone (CZ) were collected, and human adenocarcinoma lung cell cultures (A549) were exposed to a sublethal PM10 concentration (10 μg/cm(2)) for 24 h and 48 h. The results showed that PM10 exposure induced an increase in F-actin stress fibers and caused the cytoplasmic stabilization of p21(CIP1/WAF1) via phosphorylation at Thr(145) and Ser(146) and the phosphorylation of ERK1/2 on Thr(202). Changes in the cell cycle or apoptosis were not observed, but an increase in β-galactosidase activity was detected. The PM10 from CZ caused more dramatic effects in lung cells. We conclude that PM10 exposure induced cytoplasmic p21(CIP1/WAF1) retention, ERK1/2 activation, cytoskeleton remodeling and the acquisition of a senescence-like phenotype in lung cells. These alterations could have mechanistic implications regarding the carcinogenic potential of PM10.

  19. Epstein-Barr virus nuclear antigen 3A promotes cellular proliferation by repression of the cyclin-dependent kinase inhibitor p21WAF1/CIP1.

    PubMed

    Tursiella, Melissa L; Bowman, Emily R; Wanzeck, Keith C; Throm, Robert E; Liao, Jason; Zhu, Junjia; Sample, Clare E

    2014-10-01

    Latent infection by Epstein-Barr virus (EBV) is highly associated with the endemic form of Burkitt lymphoma (eBL), which typically limits expression of EBV proteins to EBNA-1 (Latency I). Interestingly, a subset of eBLs maintain a variant program of EBV latency - Wp-restricted latency (Wp-R) - that includes expression of the EBNA-3 proteins (3A, 3B and 3C), in addition to EBNA-1. In xenograft assays, Wp-R BL cell lines were notably more tumorigenic than their counterparts that maintain Latency I, suggesting that the additional latency-associated proteins expressed in Wp-R influence cell proliferation and/or survival. Here, we evaluated the contribution of EBNA-3A. Consistent with the enhanced tumorigenic potential of Wp-R BLs, knockdown of EBNA-3A expression resulted in abrupt cell-cycle arrest in G0/G1 that was concomitant with conversion of retinoblastoma protein (Rb) to its hypophosphorylated state, followed by a loss of Rb protein. Comparable results were seen in EBV-immortalized B lymphoblastoid cell lines (LCLs), consistent with the previous observation that EBNA-3A is essential for sustained growth of these cells. In agreement with the known ability of EBNA-3A and EBNA-3C to cooperatively repress p14(ARF) and p16(INK4a) expression, knockdown of EBNA-3A in LCLs resulted in rapid elevation of p14(ARF) and p16I(NK4a). By contrast, p16(INK4a) was not detectably expressed in Wp-R BL and the low-level expression of p14(ARF) was unchanged by EBNA-3A knockdown. Amongst other G1/S regulatory proteins, only p21(WAF1/CIP1), a potent inducer of G1 arrest, was upregulated following knockdown of EBNA-3A in Wp-R BL Sal cells and LCLs, coincident with hypophosphorylation and destabilization of Rb and growth arrest. Furthermore, knockdown of p21(WAF1/CIP1) expression in Wp-R BL correlated with an increase in cellular proliferation. This novel function of EBNA-3A is distinct from the functions previously described that are shared with EBNA-3C, and likely contributes to the

  20. Epstein-Barr Virus Nuclear Antigen 3A Promotes Cellular Proliferation by Repression of the Cyclin-Dependent Kinase Inhibitor p21WAF1/CIP1

    PubMed Central

    Tursiella, Melissa L.; Bowman, Emily R.; Wanzeck, Keith C.; Throm, Robert E.; Liao, Jason; Zhu, Junjia; Sample, Clare E.

    2014-01-01

    Latent infection by Epstein-Barr virus (EBV) is highly associated with the endemic form of Burkitt lymphoma (eBL), which typically limits expression of EBV proteins to EBNA-1 (Latency I). Interestingly, a subset of eBLs maintain a variant program of EBV latency - Wp-restricted latency (Wp-R) - that includes expression of the EBNA-3 proteins (3A, 3B and 3C), in addition to EBNA-1. In xenograft assays, Wp-R BL cell lines were notably more tumorigenic than their counterparts that maintain Latency I, suggesting that the additional latency-associated proteins expressed in Wp-R influence cell proliferation and/or survival. Here, we evaluated the contribution of EBNA-3A. Consistent with the enhanced tumorigenic potential of Wp-R BLs, knockdown of EBNA-3A expression resulted in abrupt cell-cycle arrest in G0/G1 that was concomitant with conversion of retinoblastoma protein (Rb) to its hypophosphorylated state, followed by a loss of Rb protein. Comparable results were seen in EBV-immortalized B lymphoblastoid cell lines (LCLs), consistent with the previous observation that EBNA-3A is essential for sustained growth of these cells. In agreement with the known ability of EBNA-3A and EBNA-3C to cooperatively repress p14ARF and p16INK4a expression, knockdown of EBNA-3A in LCLs resulted in rapid elevation of p14ARF and p16INK4a. By contrast, p16INK4a was not detectably expressed in Wp-R BL and the low-level expression of p14ARF was unchanged by EBNA-3A knockdown. Amongst other G1/S regulatory proteins, only p21WAF1/CIP1, a potent inducer of G1 arrest, was upregulated following knockdown of EBNA-3A in Wp-R BL Sal cells and LCLs, coincident with hypophosphorylation and destabilization of Rb and growth arrest. Furthermore, knockdown of p21WAF1/CIP1 expression in Wp-R BL correlated with an increase in cellular proliferation. This novel function of EBNA-3A is distinct from the functions previously described that are shared with EBNA-3C, and likely contributes to the proliferation of

  1. A Conditional Mouse Mutant in the Tumor Suppressor SdhD Gene Unveils a Link between p21WAF1/Cip1 Induction and Mitochondrial Dysfunction

    PubMed Central

    Millán-Uclés, África; Díaz-Castro, Blanca; García-Flores, Paula; Báez, Alicia; Pérez-Simón, José Antonio; López-Barneo, José; Piruat, José I.

    2014-01-01

    Mutations in mitochondrial complex II (MCII; succinate dehydrogenase, Sdh) genes cause familiar pheochromocytoma/paraganglioma tumors. Several mechanisms have been proposed to account for Sdh-mutation-induced tumorigenesis, the most accepted of which is based on the constitutive expression of the hypoxia-inducible factor 1α (Hif1α) at normal oxygen tension, a theory referred to as “pseudo-hypoxic drive”. Other molecular processes, such as oxidative stress, apoptosis, or chromatin remodeling have been also proposed to play a causative role. Nevertheless, the actual contribution of each of these mechanisms has not been definitively established. Moreover, the biological factors that determine the tissue-specificity of these tumors have not been identified. In this work, we made use of the inducible SDHD-ESR mouse, a conditional mutant in the SdhD gene, which encodes the small subunit of MCII, and that acts as a tumor suppressor gene in humans. The analysis of the Hif1α pathway in SDHD-ESR tissues and in two newly derived cell lines after complete SdhD loss -a requirement for hereditary paraganglioma type-1 tumor formation in humans- partially recapitulated the “pseudo-hypoxic” response and rendered inconsistent results. Therefore, we performed microarray analysis of adrenal medulla and kidney in order to identify other early gene expression changes elicited by SdhD deletion. Our results revealed that each mutant tissue displayed different variations in their gene expression profiles affecting to different biological processes. However, we found that the Cdkn1a gene was up-regulated in both tissues. This gene encodes the cyclin-dependent kinase inhibitor p21WAF1/Cip1, a factor implicated in cell cycle, senescence, and cancer. The two SDHD-ESR cell lines also showed accumulation of this protein. This new and unprecedented evidence for a link between SdhD dysfunction and p21WAF1/Cip1 will open new avenues for the study of the mechanisms that cause tumors in

  2. Initialized Fractional Calculus

    NASA Technical Reports Server (NTRS)

    Lorenzo, Carl F.; Hartley, Tom T.

    2000-01-01

    This paper demonstrates the need for a nonconstant initialization for the fractional calculus and establishes a basic definition set for the initialized fractional differintegral. This definition set allows the formalization of an initialized fractional calculus. Two basis calculi are considered; the Riemann-Liouville and the Grunwald fractional calculi. Two forms of initialization, terminal and side are developed.

  3. Tempered fractional calculus

    SciTech Connect

    Sabzikar, Farzad; Meerschaert, Mark M.; Chen, Jinghua

    2015-07-15

    Fractional derivatives and integrals are convolutions with a power law. Multiplying by an exponential factor leads to tempered fractional derivatives and integrals. Tempered fractional diffusion equations, where the usual second derivative in space is replaced by a tempered fractional derivative, govern the limits of random walk models with an exponentially tempered power law jump distribution. The limiting tempered stable probability densities exhibit semi-heavy tails, which are commonly observed in finance. Tempered power law waiting times lead to tempered fractional time derivatives, which have proven useful in geophysics. The tempered fractional derivative or integral of a Brownian motion, called a tempered fractional Brownian motion, can exhibit semi-long range dependence. The increments of this process, called tempered fractional Gaussian noise, provide a useful new stochastic model for wind speed data. A tempered fractional difference forms the basis for numerical methods to solve tempered fractional diffusion equations, and it also provides a useful new correlation model in time series.

  4. Lightning accommodation systems for wind turbine generator safety

    NASA Technical Reports Server (NTRS)

    Bankaitis, H.

    1981-01-01

    The wind turbine safety program identifies the naturally occurring lightning phenomenon as a hazard with the potential to cause loss of program objectives, injure personnel, damage system instrumentation, structure or support equipment and facilities. Several candidate methods of lightning accommodation for each blade were designed, analyzed, and tested by submitting sample blade sections to simulated lightning. Lightning accommodation systems for composite blades were individually developed. Their effectiveness was evaluated by submitting the systems to simulated lightning strikes. The test data were analyzed and system designs were reviewed on the basis of the analysis. This activity is directed at defining design and procedural constraints, requirements for safety devices and warning methods, special procedures, protective equipment and personnel training.

  5. Accommodation coefficient of HOBr on deliquescent sodium bromide aerosol particles

    NASA Astrophysics Data System (ADS)

    Wachsmuth, M.; Gäggeler, H. W.; von Glasow, R.; Ammann, M.

    2002-06-01

    Uptake of HOBr on sea salt aerosol, sea salt brine or ice is believed to be a key process providing a source of photolabile bromine (Br2) and sustaining ozone depletion cycles in the Arctic troposphere. In the present study, uptake of HOBr on sodium bromide (NaBr) aerosol particles was investigated at an extremely low HOBr concentration of 300 cm-3 using the short-lived radioactive isotopes 83-86Br. Under these conditions, at maximum one HOBr molecule was taken up per particle. The rate of uptake was clearly limited by the mass accommodation coefficient, which was calculated to be 0.6 ± 0.2. This value is a factor of 10 larger than estimates used in earlier models. The atmospheric implications are discussed using the box model "MOCCA'', showing that the increase of the accommodation coefficient of HOBr by a factor of 10 only slightly affects net ozone loss, but significantly increases chlorine release.

  6. Shape optimization of an accommodative intra-ocular lens

    NASA Astrophysics Data System (ADS)

    Jouve, François; Hanna, Khalil

    2005-03-01

    Cataract surgery consists in replacing the clouded or opacified crystalline lens by an Intra-Ocular Lens (IOL) having the same mean dioptrical power. Clear vision is then achieved at a given distance and glasses are needed in many situations. A new kind of IOL, potentially accommodative, is proposed. Its design is based on the deep understanding of the accommodation mechanism and on the mathematical modeling and the numerical simulation of the IOL's comportment in vivo. A preliminary version of this IOL is now commercialized by the company HumanOptics under the name '1CU'. In a second phase, shape optimization techniques equipped with strong mechanical and physiological constraints, are used to enhance the IOL performance and build a new design. To cite this article: F. Jouve, K. Hanna, C. R. Mecanique 333 (2005).

  7. CHOA concussion consensus: establishing a uniform policy for academic accommodations.

    PubMed

    Popoli, David Michael; Burns, Thomas G; Meehan, William P; Reisner, Andrew

    2014-03-01

    Concussion research generally centers on physical challenges, though aspects such as social functioning and returning to school also warrant attention in pediatric populations. Restoring academic performance postconcussion remains a challenge. Here we provide recommendations addressing a uniform policy for pediatric concussion patients in academic institutions. Tools that may minimize difficulty with academic re-entry include independent educational evaluations, individualized educational programs (IEPs), student support teams (SSTs), letters of academic accommodation, time off, and 504 Plans. Recognition and treatment is crucial for symptom relief and prevention of functional disruption, as is specialist referral during the acute window. We recommend early intervention with a letter of academic accommodation and SST and suggest that 504 Plans and IEPs be reserved for protracted or medically complicated cases. Students with concussion should be observed for anxiety and depression because these symptoms can lead to prolonged recovery, decreased quality of life, and other social challenges.

  8. The visual accommodation response during concurrent mental activity

    NASA Technical Reports Server (NTRS)

    Malmstrom, F. V.; Randle, R. J.; Bendix, J. S.; Weber, R. J.

    1980-01-01

    The direction and magnitude of the human visual accommodation response during concurrent mental activity are investigated. Subject focusing responses to targets at distances of 0.0 D, 3.0 D and an indeterminate distance were monitored by means of an optometer during the performance of a backwards counting task and a visual imagery task (thinking near and thinking far). In both experiments a shift in accommodation towards the visual far point is observed particularly for the near target, which increases with the duration of the task. The results can be interpreted in terms of both the capacity model of Kahneman (1973) and the autonomic arousal model of Hess and Polt (1964), and are not inconsistent with the possibility of an intermediate resting position.

  9. Visual performance with accommodating and multifocal intraocular lenses

    PubMed Central

    Lan, Jie; Huang, Yu-Sen; Dai, Yun-Hai; Wu, Xiao-Ming; Sun, Jia-Jun; Xie, Li-Xin

    2017-01-01

    AIM To compare the visual functional outcomes with accommodating and multifocal intraocular lenses (IOLs). METHODS Our retrospective comparative study included 51 patients (60 eyes) received implantation of an accommodating IOL (Tetraflex; 16 patients, 20 eyes), a refractive multifocal IOL (ReZoom; 18 patients, 20 eyes), or a diffractive multifocal IOL (ZMA00; 17 patients, 20 eyes). Subjective refraction, visual acuity, contrast sensitivity (CS), intraocular aberration, and subjective photic phenomena were detected at 3mo after surgery. RESULTS The spherical equivalent in the three groups was -0.38±0.54 D, 0.14±0.56 D, and 0.35±0.41 D, respectively. No statistically significant differences were found in uncorrected and corrected distance visual acuity and uncorrected intermediate visual acuity among the groups (P=0.39). The ReZoom group had significantly better distance-corrected intermediate visual acuity than the ZMA00 group (P=0.003). The ZMA00 group had significantly better near visual acuity than the other groups (P<0.05). Better contrast sensitivity values were observed in the Tetraflex group under most of the spatial frequencies conditions (P=0.025). The total aberration was lowest in the ZMA00 group (P=0.000), and the spherical aberration was highest in the Tetraflex group (P=0.000). The three groups had similar frequency of ghosting and glare, and the Tetraflex group had a low rate of halos (P=0.01). CONCLUSION Both accommodating and multifocal IOLs can successfully restore distance and uncorrected intermediate visual acuities. Tetraflex accommodating IOLs perform better in CS and with less halos of photic phenomena. ReZoom refractive multifocal IOLs have better performance in distance-corrected intermediate visual acuity than ZMA00 diffractive multifocal IOLs, and the latter achieved better near visual acuity and efficiently decreased the optical aberration. PMID:28251082

  10. Bimatoprost (0.03%)-induced accommodative spasm and pseudomyopia.

    PubMed

    Padhy, Debananda; Rao, Aparna

    2015-11-23

    Bimatoprost is a prostaglandin analogue used topically in the treatment of glaucoma. Commonly known side effects include eyelash growth, iris pigmentation and conjunctival hyperemia. While pseudomyopia is reported to be caused by parasympathomimetics, such an effect precipitated by bimatoprost has not yet been reported. We report a case demonstrating pseudomyopia and accommodative spasm caused after starting bimatoprost 0.03% in a young patient with glaucoma.

  11. Digital electronic engine control fault detection and accommodation flight evaluation

    NASA Technical Reports Server (NTRS)

    Baer-Ruedhart, J. L.

    1984-01-01

    The capabilities and performance of various fault detection and accommodation (FDA) schemes in existing and projected engine control systems were investigated. Flight tests of the digital electronic engine control (DEEC) in an F-15 aircraft show discrepancies between flight results and predictions based on simulation and altitude testing. The FDA methodology and logic in the DEEC system, and the results of the flight failures which occurred to date are described.

  12. Holocene reef development where wave energy reduces accommodation

    USGS Publications Warehouse

    Grossman, Eric E.; Fletcher, Charles H.

    2004-01-01

    Analyses of 32 drill cores obtained from the windward reef of Kailua Bay, Oahu, Hawaii, indicate that high wave energy significantly reduced accommodation space for reef development in the Holocene and produced variable architecture because of the combined influence of sea-level history and wave exposure over a complex antecedent topography. A paleostream valley within the late Pleistocene insular limestone shelf provided accommodation space for more than 11 m of vertical accretion since sea level flooded the bay 8000 yr BP. Virtually no net accretion (pile-up of fore-reef-derived rubble (rudstone) and sparse bindstone, and (3) a final stage of catch-up bindstone accretion in depths > 6 m. Coral framestone accreted at rates of 2.5-6.0 mm/yr in water depths > 11 m during the early Holocene; it abruptly terminated at ~4500 yr BP because of wave scour as sea level stabilized. More than 4 m of rudstone derived from the upper fore reef accreted at depths of 6 to 13 m below sea level between 4000 and 1500 yr BP coincident with late Holocene relative sea-level fall. Variations in the thickness, composition, and age of these reef facies across spatial scales of 10-1000 m within Kailua Bay illustrate the importance of antecedent topography and wave-related stress in reducing accommodation space for reef development set by sea level. Although accommodation space of 6 to 17 m has existed through most of the Holocene, the Kailua reef has been unable to catch up to sea level because of persistent high wave stress.

  13. Recent Religious Accommodations: Have We Gone Too Far Too Fast?

    DTIC Science & Technology

    2012-03-12

    length of head hair for both men and women , making certain allowances for women to wear longer hairstyles. The regulation prohibits the wear of beards...exceptions in order to further religious sensitivity. 10 Review of Canada, United Kingdom, and Netherlands Policy on Head and Facial Hair Some...militaries have religious accommodations for males who maintain facial and head hair for religious purposes. In addition, one nation allows soldiers to

  14. OCT-based crystalline lens topography in accommodating eyes

    PubMed Central

    Pérez-Merino, Pablo; Velasco-Ocana, Miriam; Martinez-Enriquez, Eduardo; Marcos, Susana

    2015-01-01

    Custom Spectral Domain Optical Coherence Tomography (SD-OCT) provided with automatic quantification and distortion correction algorithms was used to measure anterior and posterior crystalline lens surface elevation in accommodating eyes and to evaluate relationships between anterior segment surfaces. Nine young eyes were measured at different accommodative demands. Anterior and posterior lens radii of curvature decreased at a rate of 0.78 ± 0.18 and 0.13 ± 0.07 mm/D, anterior chamber depth decreased at 0.04 ± 0.01 mm/D and lens thickness increased at 0.04 ± 0.01 mm/D with accommodation. Three-dimensional surface elevations were estimated by subtracting best fitting spheres. In the relaxed state, the spherical term accounted for most of the surface irregularity in the anterior lens (47%) and astigmatism (70%) in the posterior lens. However, in accommodated lenses astigmatism was the predominant surface irregularity (90%) in the anterior lens. The RMS of high-order irregularities of the posterior lens surface was statistically significantly higher than that of the anterior lens surface (x2.02, p<0.0001). There was significant negative correlation in vertical coma (Z3−1) and oblique trefoil (Z3−3) between lens surfaces. The astigmatic angle showed high degree of alignment between corneal surfaces, moderate between corneal and anterior lens surface (~27 deg), but differed by ~80 deg between the anterior and posterior lens surfaces (including relative anterior/posterior lens astigmatic angle shifts (10-20 deg). PMID:26713216

  15. Accommodation response measurements for integral 3D image

    NASA Astrophysics Data System (ADS)

    Hiura, H.; Mishina, T.; Arai, J.; Iwadate, Y.

    2014-03-01

    We measured accommodation responses under integral photography (IP), binocular stereoscopic, and real object display conditions, and viewing conditions of binocular and monocular viewing conditions. The equipment we used was an optometric device and a 3D display. We developed the 3D display for IP and binocular stereoscopic images that comprises a high-resolution liquid crystal display (LCD) and a high-density lens array. The LCD has a resolution of 468 dpi and a diagonal size of 4.8 inches. The high-density lens array comprises 106 x 69 micro lenses that have a focal length of 3 mm and diameter of 1 mm. The lenses are arranged in a honeycomb pattern. The 3D display was positioned 60 cm from an observer under IP and binocular stereoscopic display conditions. The target was presented at eight depth positions relative to the 3D display: 15, 10, and 5 cm in front of the 3D display, on the 3D display panel, and 5, 10, 15 and 30 cm behind the 3D display under the IP and binocular stereoscopic display conditions. Under the real object display condition, the target was displayed on the 3D display panel, and the 3D display was placed at the eight positions. The results suggest that the IP image induced more natural accommodation responses compared to the binocular stereoscopic image. The accommodation responses of the IP image were weaker than those of a real object; however, they showed a similar tendency with those of the real object under the two viewing conditions. Therefore, IP can induce accommodation to the depth positions of 3D images.

  16. Expression of the p12 subunit of human DNA polymerase δ (Pol δ), CDK inhibitor p21(WAF1), Cdt1, cyclin A, PCNA and Ki-67 in relation to DNA replication in individual cells.

    PubMed

    Zhao, Hong; Zhang, Sufang; Xu, Dazhong; Lee, Marietta Ywt; Zhang, Zhongtao; Lee, Ernest Yc; Darzynkiewicz, Zbigniew

    2014-01-01

    We recently reported that the p12 subunit of human DNA polymerase δ (Pol δ4) is degraded by CRL4(Cdt2) which regulates the licensing factor Cdt1 and p21(WAF1) during the G1 to S transition. Presently, we performed multiparameter laser scanning cytometric analyses of changes in levels of p12, Cdt1 and p21(WAF1), detected immunocytochemically in individual cells, vis-à-vis the initiation and completion of DNA replication. The latter was assessed by pulse-labeling A549 cells with the DNA precursor ethynyl-2'-deoxyribose (EdU). The loss of p12 preceded the initiation of DNA replication and essentially all cells incorporating EdU were p12 negative. Completion of DNA replication and transition to G2 phase coincided with the re-appearance and rapid rise of p12 levels. Similar to p12 a decline of p21(WAF1) and Cdt1 was seen at the end of G1 phase and all DNA replicating cells were p21(WAF1) and Cdt1 negative. The loss of p21(WAF1) preceded that of Cdt1 and p12 and the disappearance of the latter coincided with the onset of DNA replication. Loss of p12 leads to conversion of Pol δ4 to its trimeric form, Pol δ3, so that the results provide strong support to the notion that Pol δ3 is engaged in DNA replication during unperturbed progression through the S phase of cell cycle. Also assessed was a correlation between EdU incorporation, likely reflecting the rate of DNA replication in individual cells, and the level of expression of positive biomarkers of replication cyclin A, PCNA and Ki-67 in these cells. Of interest was the observation of stronger correlation between EdU incorporation and expression of PCNA (r = 0.73) than expression of cyclin A (r = 0.47) or Ki-67 (r = 0.47).

  17. Columbus stowage optimization by cast (cargo accommodation support tool)

    NASA Astrophysics Data System (ADS)

    Fasano, G.; Saia, D.; Piras, A.

    2010-08-01

    A challenging issue related to the International Space Station utilization concerns the on-board stowage, implying a strong impact on habitability, safety and crew productivity. This holds in particular for the European Columbus laboratory, nowadays also utilized to provide the station with logistic support. The volume exploitation has to be maximized, in compliance with the given accommodation rules. At each upload step, the stowage problem must be solved quickly and efficiently. This leads to the comparison of different scenarios to select the most suitable one. Last minute upgrades, due to possible re-planning, may, moreover arise, imposing the further capability to rapidly readapt the current solution to the updated status. In this context, looking into satisfactory solutions represents a very demanding job, even for experienced designers. Thales Alenia Space Italia has achieved a remarkable expertise in the field of cargo accommodation and stowage. The company has recently developed CAST, a dedicated in-house software tool, to support the cargo accommodation of the European automated transfer vehicle. An ad hoc version, tailored to the Columbus stowage, has been further implemented and is going to be used from now on. This paper surveys the on-board stowage issue, pointing out the advantages of the proposed approach.

  18. Dead horse graben: A west Texas accommodation zone

    NASA Astrophysics Data System (ADS)

    Maler, Michael O.

    1990-12-01

    The Cretaceous rocks of the Dead Horse graben area underwent two superposed deformations. Laramide (latest Cretaceous to early Tertiary) left-transpression structures are overprinted by a Basin and Range (Miocene to Recent) accommodation zone. During Laramide shortening, two northwest trending sets of monoclines developed: an en echelon set over a left-slip basement fault and a single monocline draped over the uplifted edge of a rotated basement block. Basin and Range extension caused northerly trending half-grabens that have opposing asymmetry: downthrown-to-the-east north of the study area and downthrown-to-the-west south of the area. These half-grabens are separated by an accommodation zone, expressed as a northwest trending, right-lateral pull-apart graben. The normal faults of the pull-apart graben flatten at a shallower depth than those of the half-grabens. This suggests not only that accommodation zones can be mechanically layered but that this layering can occur on a scale different from the adjacent half-grabens. The northwest trend and superposition of both sets of structures implies a tectonic control by the preexisting structural fabric of the Texas Lineament.

  19. Development of Fraction Comparison Strategies: A Latent Transition Analysis.

    PubMed

    Rinne, Luke F; Ye, Ai; Jordan, Nancy C

    2017-02-20

    The present study investigated the development of fraction comparison strategies through a longitudinal analysis of children's responses to a fraction comparison task in 4th through 6th grades (N = 394). Participants were asked to choose the larger value for 24 fraction pairs blocked by fraction type. Latent class analysis of performance over item blocks showed that most children initially exhibited a "whole number bias," indicating that larger numbers in numerators and denominators produce larger fraction values. However, some children instead chose fractions with smaller numerators and denominators, demonstrating a partial understanding that smaller numbers can yield larger fractions. Latent transition analysis showed that most children eventually adopted normative comparison strategies. Children who exhibited a partial understanding by choosing fractions with smaller numbers were more likely to adopt normative comparison strategies earlier than those with larger number biases. Controlling for general math achievement and other cognitive abilities, whole number line estimation accuracy predicted the probability of transitioning to normative comparison strategies. Exploratory factor analyses showed that over time, children appeared to increasingly represent fractions as discrete magnitudes when simpler strategies were unavailable. These results support the integrated theory of numerical development, which posits that an understanding of numbers as magnitudes unifies the process of learning whole numbers and fractions. The findings contrast with conceptual change theories, which propose that children must move from a view of numbers as counting units to a new view that accommodates fractions to overcome whole number bias. (PsycINFO Database Record

  20. Cooperative role between p21cip1/waf1 and p27kip1 in premature senescence in glandular proliferative lesions in mice.

    PubMed

    García-Fernández, R A; García-Palencia, P; Suarez, C; Sánchez, M A; Gil-Gómez, G; Sánchez, B; Rollán, E; Martín-Caballero, J; Flores, J M

    2014-03-01

    Cellular senescence has been considered a novel target for cancer therapy. It has also been pointed out that p21(cip1/waf1) and p27(kip1) cyclin-dependent kinase inhibitors (CKIs) play a role in cellular senescence in some tumor types. Therefore, in order to address the possibility of a cooperative role between p21 and p27 proteins in senescence in vivo we analyzed cellular senescence in spontaneous glandular proliferative lesions (adrenal, thyroid and pituitary glands) in a double-KO mice model, using γH2AX, p53, p16, PTEN and Ki67 as senescence markers. The results obtained showed that p21p27 double-null mice had the lowest number of γH2AX positive cells in glandular hyperplasias and benign tumors. Also, in this group, Ki67 proliferation index correlated with a lower immunohistochemical expression of γH2AX and p53. The expression of p16 and PTEN do not seem to cause synergism of senescence in the benign lesions analyzed in p21p27 double-KO mice. These observations suggest an intrinsic cooperation between p21 and p27 CKIs in the activation of stress-induced cellular senescence and tumor progression in vivo, which would be a physiological mechanism to prevent tumor cell proliferation.

  1. Lenalidomide inhibits the proliferation of CLL cells via a cereblon/p21WAF1/Cip1-dependent mechanism independent of functional p53

    PubMed Central

    Fecteau, Jessie-F.; Corral, Laura G.; Ghia, Emanuela M.; Gaidarova, Svetlana; Futalan, Diahnn; Bharati, Ila Sri; Cathers, Brian; Schwaederlé, Maria; Cui, Bing; Lopez-Girona, Antonia; Messmer, Davorka

    2014-01-01

    Lenalidomide has demonstrated clinical activity in patients with chronic lymphocytic leukemia (CLL), even though it is not cytotoxic for primary CLL cells in vitro. We examined the direct effect of lenalidomide on CLL-cell proliferation induced by CD154-expressing accessory cells in media containing interleukin-4 and -10. Treatment with lenalidomide significantly inhibited CLL-cell proliferation, an effect that was associated with the p53-independent upregulation of the cyclin-dependent kinase inhibitor, p21WAF1/Cip1 (p21). Silencing p21 with small interfering RNA impaired the capacity of lenalidomide to inhibit CLL-cell proliferation. Silencing cereblon, a known molecular target of lenalidomide, impaired the capacity of lenalidomide to induce expression of p21, inhibit CD154-induced CLL-cell proliferation, or enhance the degradation of Ikaros family zinc finger proteins 1 and 3. We isolated CLL cells from the blood of patients before and after short-term treatment with low-dose lenalidomide (5 mg per day) and found the leukemia cells were also induced to express p21 in vivo. These results indicate that lenalidomide can directly inhibit proliferation of CLL cells in a cereblon/p21-dependent but p53-independent manner, at concentrations achievable in vivo, potentially contributing to the capacity of this drug to inhibit disease-progression in patients with CLL. PMID:24990888

  2. Lenalidomide inhibits the proliferation of CLL cells via a cereblon/p21(WAF1/Cip1)-dependent mechanism independent of functional p53.

    PubMed

    Fecteau, Jessie-F; Corral, Laura G; Ghia, Emanuela M; Gaidarova, Svetlana; Futalan, Diahnn; Bharati, Ila Sri; Cathers, Brian; Schwaederlé, Maria; Cui, Bing; Lopez-Girona, Antonia; Messmer, Davorka; Kipps, Thomas J

    2014-09-04

    Lenalidomide has demonstrated clinical activity in patients with chronic lymphocytic leukemia (CLL), even though it is not cytotoxic for primary CLL cells in vitro. We examined the direct effect of lenalidomide on CLL-cell proliferation induced by CD154-expressing accessory cells in media containing interleukin-4 and -10. Treatment with lenalidomide significantly inhibited CLL-cell proliferation, an effect that was associated with the p53-independent upregulation of the cyclin-dependent kinase inhibitor, p21(WAF1/Cip1) (p21). Silencing p21 with small interfering RNA impaired the capacity of lenalidomide to inhibit CLL-cell proliferation. Silencing cereblon, a known molecular target of lenalidomide, impaired the capacity of lenalidomide to induce expression of p21, inhibit CD154-induced CLL-cell proliferation, or enhance the degradation of Ikaros family zinc finger proteins 1 and 3. We isolated CLL cells from the blood of patients before and after short-term treatment with low-dose lenalidomide (5 mg per day) and found the leukemia cells were also induced to express p21 in vivo. These results indicate that lenalidomide can directly inhibit proliferation of CLL cells in a cereblon/p21-dependent but p53-independent manner, at concentrations achievable in vivo, potentially contributing to the capacity of this drug to inhibit disease-progression in patients with CLL.

  3. Fraction Sense: Foundational Understandings.

    PubMed

    Fennell, Francis Skip; Karp, Karen

    2016-08-09

    The intent of this commentary is to identify elements of fraction sense and note how the research studies provided in this special issue, in related but somewhat different ways, validate the importance of such understandings. Proficiency with fractions serves as a prerequisite for student success in higher level mathematics, as well as serving as a gateway to many occupations and varied contexts beyond the mathematics classroom. Fraction sense is developed through instructional opportunities involving fraction equivalence and magnitude, comparing and ordering fractions, using fraction benchmarks, and computational estimation. Such foundations are then extended to operations involving fractions and decimals and applications involving proportional reasoning. These components of fraction sense are all addressed in the studies provided in this issue, with particular consideration devoted to the significant importance of the use of the number line as a central representational tool for conceptually understanding fraction magnitude.

  4. TEMPERED FRACTIONAL CALCULUS

    PubMed Central

    MEERSCHAERT, MARK M.; SABZIKAR, FARZAD; CHEN, JINGHUA

    2014-01-01

    Fractional derivatives and integrals are convolutions with a power law. Multiplying by an exponential factor leads to tempered fractional derivatives and integrals. Tempered fractional diffusion equations, where the usual second derivative in space is replaced by a tempered fractional derivative, govern the limits of random walk models with an exponentially tempered power law jump distribution. The limiting tempered stable probability densities exhibit semi-heavy tails, which are commonly observed in finance. Tempered power law waiting times lead to tempered fractional time derivatives, which have proven useful in geophysics. The tempered fractional derivative or integral of a Brownian motion, called a tempered fractional Brownian motion, can exhibit semi-long range dependence. The increments of this process, called tempered fractional Gaussian noise, provide a useful new stochastic model for wind speed data. A tempered difference forms the basis for numerical methods to solve tempered fractional diffusion equations, and it also provides a useful new correlation model in time series. PMID:26085690

  5. Microtox Aquatic Toxcity of Petrodiesel and Biodiesel Blends: The Role of Biodiesel's Autoxidation Products

    EPA Science Inventory

    The acute Microtox toxicity of the water accommodated fraction (WAF) of six commercial soybean biodiesel/petrodiesel blends was investigated at different oil loads. We analyzed five fatty acid methyl esters (FAMEs), C10 - C24 n-alkanes, four aromatics, methanol, and tota...

  6. The acute toxicity of chemically and physically dispersed crude oil to key arctic species under arctic conditions during the open water season

    PubMed Central

    Gardiner, William W; Word, Jack Q; Word, Jack D; Perkins, Robert A; McFarlin, Kelly M; Hester, Brian W; Word, Lucinda S; Ray, Collin M

    2013-01-01

    The acute toxicity of physically and chemically dispersed crude oil and the dispersant Corexit 9500 were evaluated for key Arctic species. The copepod Calanus glacialis, juvenile Arctic cod (Boreogadus saida), and larval sculpin (Myoxocephalus sp.) were tested under conditions representative of the Beaufort and Chukchi Seas during the ice-free season. The toxicity of 3 water-accommodated fractions (WAF) of Alaska North Slope crude oil was examined with spiked, declining exposures. A dispersant-only test was conducted with the copepod C. glacialis. Each preparation with oil (WAF, breaking wave WAF [BWWAF], and chemically enhanced WAF [CEWAF]) produced distinct suites of hydrocarbon constituents; the total concentrations of oil were lowest in WAF and highest in CEWAF preparations. The relative sensitivity for the different species and age classes was similar within each WAF type. Median lethal concentration values based on total petroleum hydrocarbons ranged from 1.6 mg/L to 4.0 mg/L for WAF and BWWAF treatments and from 22 mg/L to 62 mg/L for CEWAF. For Corexit 9500 exposures, median lethal concentration values ranged from 17 mg/L to 50 mg/L. The differences in the relative toxicity among the accommodated fractions indicated that the majority of petroleum hydrocarbons in the CEWAF are in less acutely toxic forms than the components that dominate the WAF or BWWAF. Further evaluation showed that the parent polycyclic aromatic hydrocarbon compounds, specifically naphthalene, were highly correlated to acute toxicity. Environ Toxicol Chem 2013;32:2284–2300. PMID:23765555

  7. DIY Fraction Pack.

    ERIC Educational Resources Information Center

    Graham, Alan; Graham, Louise

    2003-01-01

    Describes a very successful attempt to teach fractions to year 5 pupils based on pupils making their own fraction pack. Children decided for themselves how to make the fractional slices used in the activity using colored cardboard sheets and templates of a paper circle consisting of 24 equal slices. (Author/NB)

  8. Accommodation Decision Making for Postsecondary Students With Learning Disabilities: Individually Tailored or One Size Fits All?

    PubMed

    Weis, Robert; Dean, Emily L; Osborne, Karen J

    2016-09-01

    Clinicians uniformly recommend accommodations for college students with learning disabilities; however, we know very little about which accommodations they select and the validity of their recommendations. We examined the assessment documentation of a large sample of community college students receiving academic accommodations for learning disabilities to determine (a) which accommodations their clinicians recommended and (b) whether clinicians' recommendations were supported by objective data gathered during the assessment process. In addition to test and instructional accommodations, many clinicians recommended that students with learning disabilities should have different educational expectations, standards, and methods of evaluation (i.e., grading) than their nondisabled classmates. Many of their recommendations for accommodations were not supported by objective evidence from students' history, diagnosis, test data, and current functioning. Furthermore, clinicians often recommended accommodations that were not specific to the student's diagnosis or area of disability. Our findings highlight the need for individually selected accommodations matched to students' needs and academic contexts.

  9. Accommodation of angular incompatibilities between interfacial facets during precipitate growth

    NASA Astrophysics Data System (ADS)

    Pond, R. C.; Jiao, H.; Zhang, L. C.; Aindow, M.

    2006-03-01

    Precipitate growth in a duplex stainless steel and a titanium aluminide alloy has been studied using transmission electron microscopy. Particles with similar crystallography and acicular form arise in both cases, and are bounded by two principal facets. One facet, designated C, is based on a commensurate singular interface structure, and the coherency strains are accommodated by interfacial defects. The other facet, designated I, is based on a singular configuration that is incommensurate in one dimension. The orientation relationship (OR) between the particle and the matrix for the singular C structure is Kurdjumov-Sachs (K-S), whereas that for the I facet is Pitsch. The angular incompatibility between these two types of facets must be accommodated to minimize the displacement field as particles grow. The present observations suggest that this is accomplished through the generation of crystal dislocations at facet junctions and their subsequent climb along the facets. The total density of defects needed to accommodate the angular discrepancy is fixed, but the partitioning of these dislocations between C and I facets is not. The actual partitioning determines the observed OR for a particle, and is determined by the kinetics of climb, which is likely to be different in the two facets. In the stainless steel, the observations are consistent with climb occurring in both the C and I facets, but faster in the I facet, leading to a distribution of observed ORs that is skewed away from Pitsch toward K-S. In the titanium aluminide alloy, no climb into the C facets was found, so a unique OR close to K-S arises.

  10. Functionalised polysiloxanes as injectable, in situ curable accommodating intraocular lenses.

    PubMed

    Hao, Xiaojuan; Jeffery, Justine L; Wilkie, John S; Meijs, Gordon F; Clayton, Anthony B; Watling, Jason D; Ho, Arthur; Fernandez, Viviana; Acosta, Carolina; Yamamoto, Hideo; Aly, Mohamed G M; Parel, Jean-Marie; Hughes, Timothy C

    2010-11-01

    The aged eye's ability to change focus (accommodation) may be restored by replacing the hardened natural lens with a soft gel. Functionalised polysiloxane macromonomers, designed for application as an injectable, in situ curable accommodating intraocular lens (A-IOL), were prepared via a two-step synthesis. Prepolymers were synthesised via ring opening polymerisation (ROP) of octamethylcyclotetrasiloxane (D(4)) and 2,4,6,8-tetramethylcyclotetrasiloxane (D(4)(H)) in toluene using trifluoromethanesulfonic acid (TfOH) as catalyst. Hexaethyldisiloxane (HEDS) was used as the end group to control the molecular weight of the prepolymers, which were then converted to macromonomers by hydrosilylation of the SiH groups with allyl methacrylate (AM) to introduce polymerisable groups. The resulting macromonomers had an injectable consistency and thus, were able to be injected into and refill the empty lens capsular bag. The macromonomers also contained a low ratio of polymerisable groups so that they may be cured on demand, in situ, under irradiation of blue light, in the presence of a photo-initiator, to form a soft polysiloxane gel (an intraocular lens) in the eye. The pre-cure viscosity and post-cure modulus of the polysiloxanes, which are crucial factors for an injectable, in situ curable A-IOL application, were controlled by adjusting the end group and D(4)(H) concentrations, respectively, in the ROP. The macromonomers were fully cured within 5 min under light irradiation, as shown by the rapid change in modulus monitored by photo-rheology. Ex vivo primate lens stretching experiments on an Ex Vivo Accommodation Simulator (EVAS) showed that the polysiloxane gel refilled lenses achieved over 60% of the accommodation amplitude of the natural lens. An in vivo biocompatibility study in rabbits using the lens refilling (Phaco-Ersatz) procedure demonstrated that the soft gels had good biocompatibility with the ocular tissue. The polysiloxane macromonomers meet the targeted

  11. Early space station user accommodations. [manned free flying orbital platform

    NASA Technical Reports Server (NTRS)

    Saxton, D. R.; Wolbers, H. L.

    1975-01-01

    The requirements for extended-duration space missions in earth orbit beyond those anticipated for the 7- to 30-day Shuttle Spacelab system have been examined. It has been determined that a continuously manned, free-flying orbital facility provides a realistic and cost-effective space platform for multidiscipline payloads designed to support research, applications, and system implementation programs such as the assembly of large space structures, and on-orbit space manufacturing. A conceptual description of such a manned facility (early Space Station) and the accommodations that it can provide using agencies and organizations is presented in this paper.

  12. Plant and animal accommodation for Space Station Laboratory

    NASA Technical Reports Server (NTRS)

    Olson, Richard L.; Gustan, Edith A.; Wiley, Lowell F.

    1986-01-01

    An extended study has been conducted with the goals of defining and analyzing relevant parameters and significant tradeoffs for the accommodation of nonhuman research aboard the NASA Space Station, as well as conducting tradeoff analyses for orbital reconfiguring or reoutfitting of the laboratory facility and developing laboratory designs and program plans. The two items exerting the greatest influence on nonhuman life sciences research were identified as the centrifuge and the specimen environmental control and life support system; both should be installed on the ground rather than in orbit.

  13. Space station accommodations for lunar base elements: A study

    NASA Technical Reports Server (NTRS)

    Weidman, Deene J.; Cirillo, William; Llewellyn, Charles; Kaszubowski, Martin; Kienlen, E. Michael, Jr.

    1987-01-01

    The results of a study conducted at NASA-LaRC to assess the impact on the space station of accommodating a Manned Lunar Base are documented. Included in the study are assembly activities for all infrastructure components, resupply and operations support for lunar base elements, crew activity requirements, the effect of lunar activities on Cape Kennedy operations, and the effect on space station science missions. Technology needs to prepare for such missions are also defined. Results of the study indicate that the space station can support the manned lunar base missions with the addition of a Fuel Depot Facility and a heavy lift launch vehicle to support the large launch requirements.

  14. Gradiometer accommodation on board a drag-free satellite

    NASA Technical Reports Server (NTRS)

    Touboul, P.

    1987-01-01

    Accommodation of the GRADIO instrument, composed of eight three-axis ultrasensitive accelerometers located at the corners of a cubic structure on a Geopotential Research Mission spacecraft is discussed. A two-stage DISCOS configuration with a magnetically suspended inner-stage; and a very fine control of the Earth pointed inner-stage taking into account the GRADIO accelerometer measurements in order to limit the variations of the angular accelerations are required. For diagonal tensor components and an averaging time of 4 sec, the overall goal is 0.1 Eotvos at 160 km altitude.

  15. Accommodating and cracking mechanisms in low-cycle fatigue

    NASA Technical Reports Server (NTRS)

    Pineau, A.

    1978-01-01

    The three main stages of fatigue life (accommodation, crack initiation and crack growth) are briefly reviewed. The cyclic behavior of annealed or predeformed face-centered cubic metals is described. Moreover, two types of alloys (Al-4-Cu and WASPALOY) are examined regarding the influence of the interactions between the precipitates and the dislocations on the cyclic behavior. Data on the percent of life to crack initiation (for a microcrack smaller than about 100 microns) are also given. Finally, experimental and theoretical results on crack growth rates in lowcycle fatigue are described.

  16. Maxwell boundary condition and velocity dependent accommodation coefficient

    SciTech Connect

    Struchtrup, Henning

    2013-11-15

    A modification of Maxwell's boundary condition for the Boltzmann equation is developed that allows to incorporate velocity dependent accommodation coefficients into the microscopic description. As a first example, it is suggested to consider the wall-particle interaction as a thermally activated process with three parameters. A simplified averaging procedure leads to jump and slip boundary conditions for hydrodynamics. Coefficients for velocity slip, temperature jump, and thermal transpiration flow are identified and compared with those resulting from the original Maxwell model and the Cercignani-Lampis model. An extension of the model leads to temperature dependent slip and jump coefficients.

  17. Flight accommodations using a special structure. [space shuttle payloads support

    NASA Technical Reports Server (NTRS)

    Noblitt, B. G.; Mcannally, R.

    1982-01-01

    A special payload carrier structure has been developed in order to provide Space Shuttle flight accommodations for an exceptionally heavy instrument package requiring no subsystems support. This Mission Peculiar Equipment Support Structure (MPESS) will support the OSTA-2 payload for a materials processing mission. The modular design of the MPESS offers a payload support capability at multiple locations within the Space Shuttle cargo bay. The MPESS is also scheduled for use with earth observation instruments to be carried by the OSTA-3 mission in late 1984.

  18. Pitch fractionation. Technical report

    SciTech Connect

    Weinberg, V.L.; White, J.L.

    1981-12-15

    Petroleum pitch (Ashland A240) has been subjected to thermal treatment and solvent fractionation to produce refined pitches to be evaluated as impregnants for carbon-carbon composites. The solvent fractions were obtained by sequential Soxhlet extraction with solvents such as hexane, cyclohexane, toluene, and pyridine. The most severe thermal treatment produced a mesophase pitch (approximately 50% mesophase); an appreciable portion of the mesophase was soluble in strong solvents. There were substantial differences in chemical composition and in pyrolysis behavior of the fractions. As the depth of fraction increased, the pyrolysis yield and bloating increased, and the microstructure of the coke became finer until glassy microconstituents were formed in the deepest fractions.

  19. Origin of picritic green glass magmas by polybaric fractional fusion

    NASA Technical Reports Server (NTRS)

    Longhi, John

    1992-01-01

    A preliminary presentation is given of a model which explains the picritic lunar mare green glass compositions as composites or magmas derived by polybaric fractional melting. The model accommodates changes in source composition and solidus temperature. As in the Klein and Langmuir model, the final compositions of the melts signify only average depths of melting. The onset of melting is deeper, final segregation is shallower.

  20. 75 FR 65261 - Virginia Graeme Baker Pool and Spa Safety Act; Public Accommodation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-22

    ... ``public accommodations facility'' as used in the Virginia Graeme Baker Pool and Spa Safety Act. DATES... or spa that is open exclusively to patrons of a hotel or other public accommodations facility.'' Section 1404(c)(2)(B)(iii) of the Act. The Act does not define the term ``public accommodations...

  1. 36 CFR 1005.9 - Discrimination in furnishing public accommodations and transportation services.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., inn, lodge, or other facility or accommodation offered to or enjoyed by the general public within the... national origin in furnishing or refusing to furnish such person or persons any accommodation, facility... will be conspicuous to any person seeking accommodations, facilities, services, or privileges:...

  2. 75 FR 12167 - Virginia Graeme Baker Pool and Spa Safety Act; Public Accommodation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-15

    ... is ``open exclusively to patrons of a hotel or other public accommodations facility.'' Section 1404(c)(2)(B)(iii) of the Act. The term ``public accommodations facility'' is not defined in the Act. The Commission has received numerous inquiries regarding what constitutes a public accommodations facility...

  3. 36 CFR 5.9 - Discrimination in furnishing public accommodations and transportation services.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... employees of any hotel, inn, lodge, or other facility or accommodation offered to or enjoyed by the general... vehicle service and its employees, are prohibited from: (1) Publicizing the facilities, accommodations or... origin in furnishing or refusing to furnish such person or persons any accommodation, facility,...

  4. 28 CFR 36.207 - Places of public accommodation located in private residences.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ON THE BASIS OF DISABILITY BY PUBLIC ACCOMMODATIONS AND IN COMMERCIAL FACILITIES General Requirements § 36.207 Places of public accommodation located in private residences. (a) When a place of public... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Places of public accommodation located...

  5. Teaching Adolescent Students with Learning Disabilities to Self-Advocate for Accommodations

    ERIC Educational Resources Information Center

    Prater, Mary Anne; Redman, Ashleigh Smith; Anderson, Darlene; Gibb, Gordon S.

    2014-01-01

    In the general education classroom students with learning disabilities (LD) often need academic accommodations to be successful. These accommodations are typically selected and implemented by their general education teachers, not by the students themselves. High school students with LD were taught to recognize when an accommodation was needed,…

  6. 28 CFR 36.207 - Places of public accommodation located in private residences.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 1 2011-07-01 2011-07-01 false Places of public accommodation located in... § 36.207 Places of public accommodation located in private residences. (a) When a place of public accommodation is located in a private residence, the portion of the residence used exclusively as a residence...

  7. 46 CFR 154.330 - Openings to accommodation, service, or control spaces.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 5 2011-10-01 2011-10-01 false Openings to accommodation, service, or control spaces..., Construction and Equipment Ship Arrangements § 154.330 Openings to accommodation, service, or control spaces... accommodation, service, or control spaces, except as allowed in paragraph (c) of this section, must be: (1)...

  8. 46 CFR 153.201 - Openings to accommodation, service or control spaces.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 5 2011-10-01 2011-10-01 false Openings to accommodation, service or control spaces... and Equipment General Vessel Requirements § 153.201 Openings to accommodation, service or control... exhausts, and other openings to accommodation, service, or control spaces must be located aft of the...

  9. 46 CFR 30.10-2 - Accommodation space-TB/ALL.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 1 2014-10-01 2014-10-01 false Accommodation space-TB/ALL. 30.10-2 Section 30.10-2... Accommodation space—TB/ALL. The term accommodation space means any public space such as a hall, dining room... that contains no cooking appliances, and a similar space open to the passengers and crew....

  10. 46 CFR 30.10-2 - Accommodation space-TB/ALL.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 1 2013-10-01 2013-10-01 false Accommodation space-TB/ALL. 30.10-2 Section 30.10-2... Accommodation space—TB/ALL. The term accommodation space means any public space such as a hall, dining room... that contains no cooking appliances, and a similar space open to the passengers and crew....

  11. 46 CFR 30.10-2 - Accommodation space-TB/ALL.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 1 2012-10-01 2012-10-01 false Accommodation space-TB/ALL. 30.10-2 Section 30.10-2... Accommodation space—TB/ALL. The term accommodation space means any public space such as a hall, dining room... that contains no cooking appliances, and a similar space open to the passengers and crew....

  12. Guidelines for Providing Accommodations Using CASAS Assessment for Learners with Disabilities

    ERIC Educational Resources Information Center

    CASAS - Comprehensive Adult Student Assessment Systems (NJ1), 2005

    2005-01-01

    These guidelines address methods for administering Comprehensive Adult Student Assessment System (CASAS) assessments using accommodations for learners with documented disabilities. The suggested accommodations for disability categories include provisions for: (1) Accommodations in test administration procedures; and (2) Use of appropriate CASAS…

  13. The Role of Accommodations in Educational Accountability Systems. Topical Review Eight

    ERIC Educational Resources Information Center

    Thompson, Sandra J.; Lazarus, Sheryl S.; Thurlow, Martha L.; Clapper, Ann T.

    2005-01-01

    Many students with disabilities need to use accommodations to meaningfully access instruction and to be appropriately assessed. During the past decade, the use of assessment accommodations has dramatically increased the participation rates of students with disabilities in statewide testing. Assessment accommodations can be defined as "changes…

  14. Barriers to Accommodation Use for Students with Disabilities in Postsecondary Education

    ERIC Educational Resources Information Center

    Lyman, Michael James

    2013-01-01

    Students with disabilities at the postsecondary level face a number of different barriers to accommodation use. Past research has shown that students with disabilities that use accommodations obtain greater academic achievement and higher graduation rates. Limited research has been conducted to identify barriers to accommodation use, and the…

  15. Accommodations Quality for Students Who Are d/Deaf or Hard of Hearing

    ERIC Educational Resources Information Center

    Cawthorn, Stephanie W.; Leppo, Rachel

    2013-01-01

    Students who are d/Deaf or hard of hearing often receive accommodations that are intended to increase access to the educational environment. The authors provide the results of a large national study of accommodations use in secondary and postsecondary settings. The article focuses on three aspects of accommodations use: access, quality, and…

  16. Are high lags of accommodation in myopic children due to motor deficits?

    PubMed

    Labhishetty, Vivek; Bobier, William R

    2017-01-01

    Children with a progressing myopia exhibit an abnormal pattern of high accommodative lags coupled with high accommodative convergence (AC/A) and high accommodative adaptation. This is not predicted by the current models of accommodation and vergence. Reduced accommodative plant gain and reduced sensitivity to blur have been suggested as potential causes for this abnormal behavior. These etiologies were tested by altering parameters (sensory, controller and plant gains) in the Simulink model of accommodation. Predictions were then compared to the static and dynamic blur accommodation (BA) measures taken using a Badal optical system on 12 children (6 emmetropes and 6 myopes, 8-13years) and 6 adults (20-35years). Other critical parameters such as CA/C, AC/A, and accommodative adaptation were also measured. Usable BA responses were classified as either typical or atypical. Typical accommodation data confirmed the abnormal pattern of myopia along with an unchanged CA/C. Main sequence relationship remained invariant between myopic and nonmyopic children. An overall reduction was noted in the response dynamics such as peak velocity and acceleration with age. Neither a reduced plant gain nor reduced blur sensitivity could predict the abnormal accommodative behavior. A model adjustment reflecting a reduced accommodative sensory gain (ASG) coupled with an increased AC cross-link gain and reduced vergence adaptive gain does predict the empirical findings. Empirical measures also showed a greater frequency of errors in accommodative response generation (atypical responses) in both myopic and control children compared to adults.

  17. 14 CFR 382.81 - For which passengers must carriers make seating accommodations?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false For which passengers must carriers make seating accommodations? 382.81 Section 382.81 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF... TRAVEL Seating Accommodations § 382.81 For which passengers must carriers make seating accommodations?...

  18. 14 CFR 382.81 - For which passengers must carriers make seating accommodations?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false For which passengers must carriers make seating accommodations? 382.81 Section 382.81 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF... TRAVEL Seating Accommodations § 382.81 For which passengers must carriers make seating accommodations?...

  19. 14 CFR 382.81 - For which passengers must carriers make seating accommodations?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 4 2014-01-01 2014-01-01 false For which passengers must carriers make seating accommodations? 382.81 Section 382.81 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF... TRAVEL Seating Accommodations § 382.81 For which passengers must carriers make seating accommodations?...

  20. 14 CFR 382.81 - For which passengers must carriers make seating accommodations?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 4 2012-01-01 2012-01-01 false For which passengers must carriers make seating accommodations? 382.81 Section 382.81 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF... TRAVEL Seating Accommodations § 382.81 For which passengers must carriers make seating accommodations?...

  1. Work Barriers Experienced and Job Accommodations Used by Persons with Arthritis and Other Rheumatic Diseases.

    ERIC Educational Resources Information Center

    Allaire, Saralynn H.; Li, Wei; LaValley, Michael P.

    2003-01-01

    Many people with arthritis become work disabled, but little is known about the types of work barriers they experience and their use of job accommodations. This article describes work barriers and use of accommodations and examines factors associated with accommodation use in persons with arthritis at risk for work disability. (Contains 30…

  2. Accommodation Decision Making for Postsecondary Students with Learning Disabilities: Individually Tailored or One Size Fits All?

    ERIC Educational Resources Information Center

    Weis, Robert; Dean, Emily L.; Osborne, Karen J.

    2016-01-01

    Clinicians uniformly recommend accommodations for college students with learning disabilities; however, we know very little about which accommodations they select and the validity of their recommendations. We examined the assessment documentation of a large sample of community college students receiving academic accommodations for learning…

  3. The Use of Computer Technology in Designing Appropriate Test Accommodations for English Language Learners

    ERIC Educational Resources Information Center

    Abedi, Jamal

    2014-01-01

    Among the several forms of accommodations used in the assessment of English language learners (ELLs), language-based accommodations are the most effective in making assessments linguistically accessible to these students. However, there are significant challenges associated with the implementation of many of these accommodations. This article…

  4. Evaluating the Impact of Test Accommodations on Test Scores of LEP Students & Non-LEP Students.

    ERIC Educational Resources Information Center

    Hafner, Anne L.

    Using a quasi-experimental analysis of variance (ANOVA) design, this project examined the effects of the use of accommodations with students of limited English proficiency (LEP) and non-LEP students and whether the use of accommodations affected the validity of test score interpretations. Major accommodations examined were extra time, and extra…

  5. 46 CFR 30.10-2 - Accommodation space-TB/ALL.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false Accommodation space-TB/ALL. 30.10-2 Section 30.10-2... Accommodation space—TB/ALL. The term accommodation space means any public space such as a hall, dining room... that contains no cooking appliances, and a similar space open to the passengers and crew....

  6. 46 CFR 30.10-2 - Accommodation space-TB/ALL.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 1 2010-10-01 2010-10-01 false Accommodation space-TB/ALL. 30.10-2 Section 30.10-2... Accommodation space—TB/ALL. The term accommodation space means any public space such as a hall, dining room... that contains no cooking appliances, and a similar space open to the passengers and crew....

  7. 14 CFR 382.81 - For which passengers must carriers make seating accommodations?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false For which passengers must carriers make seating accommodations? 382.81 Section 382.81 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF... TRAVEL Seating Accommodations § 382.81 For which passengers must carriers make seating accommodations?...

  8. Expression of p21(WAF1/CIP1/SDI1) and p53 in apoptotic cells in the adrenal cortex and induction by ischemia/reperfusion injury.

    PubMed Central

    Didenko, V V; Wang, X; Yang, L; Hornsby, P J

    1996-01-01

    p21(WAF1/CIP1/SDI1), an inhibitor of cyclin-dependent kinases, is expressed at varying levels in human adrenal glands removed during surgery or organ recovery. In glands with p21 mRNA, nuclear p21 immunoreactivity, which was occasionally extensive, colocalized with p53 immunoreactivity and DNA damage, as evidenced by in situ end-labeling. Many cells showed morphological features of apoptosis when observed by fluorescent DNA dye staining and electron microscopy. This pattern was also associated with high levels of cytoplasmic heat shock protein 70. To address the question of the origin of p21 expression in some human adrenal glands, rat adrenal glands were subjected to 30 min of ischemia followed by 8 h of reperfusion. Cells with nuclear p21 and p53 appeared in the adrenal cortex together with DNA damage detected by in situ end-labeling. Nuclear p21 immunoreactivity was also produced in adrenal tissue fragments incubated at 37 degrees C in vitro. However, in this case, p21 expression was confined to the cut edge of the tissue. In contrast, p21 in human adrenal glands, as in ischemic rat glands, was within the inner regions of the cortex, supporting an origin of the protein in vivo rather than postmortem. The p53/p21 pathway of reaction to cellular injury, potentially leading to apoptosis, may play a role in tissue damage such as that resulting from ischemia/reperfusion. In the human adrenal cortex this process may be a precursor of adrenal failure. PMID:8601638

  9. CCAAT/enhancer-binding protein alpha (C/EBP alpha) inhibits cell proliferation through the p21 (WAF-1/CIP-1/SDI-1) protein.

    PubMed

    Timchenko, N A; Wilde, M; Nakanishi, M; Smith, J R; Darlington, G J

    1996-04-01

    C/EBPalpha has a role in growth arrest and differentiation of mouse preadipocytes. To study the mechanism of C/EBPalpha-induced growth arrest, we developed a cell line, HT1, that contained the human C/EBPalpha gene under Lac repressor control. IPTG-induced C/EBPalpha caused inhibition of cell proliferation and DNA synthesis as measured by colony growth assays, cell counting, and BrdU uptake. A number of proteins that are known to be involved in the regulation of the cell cycle, such as cyclin-dependent kinase (CDK)2 and CDK4, proliferating cell nuclear antigen (PCNA), p53, c-fos, and the CDK inhibitor p16 and p27 were investigated by Western analysis. No change in their expression was observed. However, the p21 (WAF-1/CIP-1/SDI-1) protein was significantly elevated in growth-arrested HT1 cells. Elevation of p21/SDI-1 mRNA (threefold) and activation of the p21/SDI-1 promoter by C/EBPalpha did not account for the 12- to 20-fold increase in p21/SDI-1 protein. Protein synthesis inhibition by cycloheximide (CHX) treatment indicated that the half-life of p21/SDI-1 in dividing HT1 cells was approximately 30 min. However, in C/EBPalpha growth-arrested cells, the level of the p21/SDI-1 did not change for > 80 min after CHX addition. Our studies demonstrate that C/EBPalpha activates p21/SDI-1 by increasing p21/SDI-1 gene expression and by post-translational stabilization of p21/SDI-1 protein. Furthermore, induction of p21/SDI-1 is responsible for the ability of C/EBPalpha to inhibit proliferation because transcription of antisense p21/SDI-1 mRNA eliminated growth inhibition by C/EBPalpha.

  10. Pim-1 kinase-dependent phosphorylation of p21Cip1/WAF1 regulates its stability and cellular localization in H1299 cells.

    PubMed

    Zhang, Yandong; Wang, Zeping; Magnuson, Nancy S

    2007-09-01

    Previous studies from our laboratory showed that p21Cip1/WAF1 can be phosphorylated by Pim-1 kinase in vitro, implying that part of the function of Pim-1 might involve influencing the cell cycle. In the present study, site-directed mutagenesis and phosphorylated-specific antibodies were used as tools to identify the sites phosphorylated by Pim-1 and the consequences of this phosphorylation. What we found was that Pim-1 can efficiently phosphorylate p21 on Thr145 in vitro using recombinant protein and in vivo in intact cells. Unexpectedly, we found that Ser146 is a second site that is phosphorylated in vivo, but this phosphorylation event seems to be an indirect result of Pim-1 expression. More importantly, the consequences of phosphorylation of either Thr145 or Ser146 are distinct. When p21 is phosphorylated on Thr145, it localizes to the nucleus and results in the disruption of the association between proliferating cell nuclear antigen and p21. Furthermore, phosphorylation of Thr145 promotes stabilization of p21. On the other hand, when p21 is phosphorylated on Ser146, it localizes primarily in the cytoplasm and the effect of phosphorylation on stability is minimal. Cotransfection of wild-type Pim-1 with p21 increases the rate of proliferation compared with cotransfection of p21 with kinase-dead Pim-1. Knocking down Pim-1 expression greatly decreases the rate of proliferation of H1299 cells and their ability to grow in soft agar. These data suggest that Pim-1 overexpression may contribute to tumorigenesis in part by influencing the cellular localization and stability of p21 and by promoting cell proliferation.

  11. Geostationary platform study: Advanced ESGP/evolutionary SSF accommodation study

    NASA Technical Reports Server (NTRS)

    1990-01-01

    The implications on the evolutionary space station of accommodating geosynchronous Earth Orbit (GEO) facilities including unmanned satellites and platforms, manned elements, and transportation and servicing vehicles/elements. The latest existing definitions of typical unmanned GEO facilities and transportation and servicing vehicles/elements are utilized. The physical design, functional design, and operations implications at the space station are determined. Various concepts of the space station from past studies are utilized ranging from the IOC Multifunction Space Station to a branched transportation node space station, and the implications of the accommodation the GEO infrastructure of each type are assessed. Where possible, parametric data are provided to show the implications of variations in sizes and quantities of elements, launch rates, crew sizes, etc. The use of advanced automation, robotics equipment, and an efficient mix of manned/automated support for accomplishing necessary activities at the space station are identified and assessed. The products of this study are configuration sketches, resource requirements, trade studies, and parametric data.

  12. Science Language Accommodation in Elementary School Read-Alouds

    NASA Astrophysics Data System (ADS)

    Glass, Rory; Oliveira, Alandeom W.

    2014-03-01

    This study examines the pedagogical functions of accommodation (i.e. provision of simplified science speech) in science read-aloud sessions facilitated by five elementary teachers. We conceive of read-alouds as communicative events wherein teachers, faced with the task of orally delivering a science text of relatively high linguistic complexity, open up an alternate channel of communication, namely oral discussion. By doing so, teachers grant students access to a simplified linguistic input, a strategy designed to promote student comprehension of the textual contents of children's science books. It was found that nearly half (46%) of the read-aloud time was allotted to discussions with an increased percentage of less sophisticated words and reduced use of more sophisticated vocabulary than found in the books through communicative strategies such as simplified rewording, simplified definition, and simplified questioning. Further, aloud reading of more linguistically complex books required longer periods of discussion and an increased degree of teacher oral input and accommodation. We also found evidence of reversed simplification (i.e. sophistication), leading to student uptake of scientific language. The main significance of this study is that it reveals that teacher talk serves two often competing pedagogical functions (accessible communication of scientific information to students and promotion of student acquisition of the specialized language of science). It also underscores the importance of giving analytical consideration to the simplification-sophistication dimension of science classroom discourse as well as the potential of computer-based analysis of classroom discourse to inform science teaching.

  13. An experimental assembly for precise measurement of thermal accommodation coefficients

    NASA Astrophysics Data System (ADS)

    Trott, Wayne M.; Castañeda, Jaime N.; Torczynski, John R.; Gallis, Michael A.; Rader, Daniel J.

    2011-03-01

    An experimental apparatus has been developed to determine thermal accommodation coefficients for a variety of gas-surface combinations. Results are obtained primarily through measurement of the pressure dependence of the conductive heat flux between parallel plates separated by a gas-filled gap. Measured heat-flux data are used in a formula based on Direct Simulation Monte Carlo (DSMC) simulations to determine the coefficients. The assembly also features a complementary capability for measuring the variation in gas density between the plates using electron-beam fluorescence. Surface materials examined include 304 stainless steel, gold, aluminum, platinum, silicon, silicon nitride, and polysilicon. Effects of gas composition, surface roughness, and surface contamination have been investigated with this system; the behavior of gas mixtures has also been explored. Without special cleaning procedures, thermal accommodation coefficients for most materials and surface finishes were determined to be near 0.95, 0.85, and 0.45 for argon, nitrogen, and helium, respectively. Surface cleaning by in situ argon-plasma treatment reduced coefficient values by up to 0.10 for helium and by ˜0.05 for nitrogen and argon. Results for both single-species and gas-mixture experiments compare favorably to DSMC simulations.

  14. Validation of a nonrigid registration framework that accommodates tissue resection

    NASA Astrophysics Data System (ADS)

    Risholm, Petter; Samset, Eigil; Wells, William, III

    2010-03-01

    We present a 3D extension and validation of an intra-operative registration framework that accommodates tissue resection. The framework is based on the bijective Demons method, but instead of regularizing with the traditional Gaussian smoother, we apply an anisotropic diffusion filter with the resection modeled as a diffusion sink. The diffusion sink prevents unwanted Demon forces that originates from the resected area from diffusing into the surrounding area. Another attractive property of the diffusion sink is the resulting continuous deformation field across the diffusion sink boundary, which allows us to move the boundary of the diffusion sink without changing values in the deformation field. The area of resection is estimated by a level-set method evolving in the space of image intensity disagreements in the intra-operative image domain. A product of using the bijective Demons method is that we can also provide an accurate estimate of the resected tissue in the preoperative image space. Validation of the proposed method was performed on a set of 25 synthetic images. Our experiments show a significant improvement in accommodating resection using the proposed method compared to two other Demons based methods.

  15. Space Station accommodation of the Space Exploration Initiative

    NASA Technical Reports Server (NTRS)

    Ahlf, Peter; Peach, Lewis; Maksimovic, Velimir

    1990-01-01

    It is pointed out that Space Station Freedom (SSF) will support the transportation, research, and development requirements of the Space Exploration Initiative through augmentation of its resources and initial capabilities. These augmentations include providing facilities for lunar and Mars vehicle testing, processing, and servicing; providing laboratories and equipment for such enabling research as microgravity countermeasures development; and providing for the additional crew that will be required to carry out these duties. It is noted that the best way to facilitate these augmentations is to ensure 'design-for-growth' capabilities by incorporating necessary design features in the baseline program. The critical items to be accommodated in the baseline design include provisions for future increased power-generation capability, the ability to add nodes and modules, and the ability to expand the truss structure to accommodate new facilities. The SSF program must also address the effect on nonexploration users (e.g., NASA experimenters, commercial users, university investigators, and international partners of the U.S.) of SSF facilities.

  16. Scaling and Accommodation of Jaw Adductor Muscles in Canidae

    PubMed Central

    Kemp, Graham J.; Jeffery, Nathan

    2016-01-01

    ABSTRACT The masticatory apparatus amongst closely related carnivoran species raises intriguing questions about the interplay between allometry, function, and phylogeny in defining interspecific variations of cranial morphology. Here we describe the gross structure of the jaw adductor muscles of several species of canid, and then examine how the muscles are scaled across the range of body sizes, phylogenies, and trophic groups. We also consider how the muscles are accommodated on the skull, and how this is influenced by differences of endocranial size. Data were collected for a suite of morphological metrics, including body mass, endocranial volume, and muscle masses and we used geometric morphometric shape analysis to reveal associated form changes. We find that all jaw adductor muscles scale isometrically against body mass, regardless of phylogeny or trophic group, but that endocranial volume scales with negative allometry against body mass. These findings suggest that head shape is partly influenced by the need to house isometrically scaling muscles on a neurocranium scaling with negative allometry. Principal component analysis suggests that skull shape changes, such as the relatively wide zygomatic arches and large sagittal crests seen in species with higher body masses, allow the skull to accommodate a relative enlargement of the jaw adductors compared with the endocranium. Anat Rec, 299:951–966, 2016. © 2016 The Authors The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology Published by Wiley Periodicals, Inc. PMID:27103346

  17. Can mergers-in-progress be unmerged in speech accommodation?

    PubMed

    Babel, Molly; McAuliffe, Michael; Haber, Graham

    2013-01-01

    This study examines spontaneous phonetic accommodation of a dialect with distinct categories by speakers who are in the process of merging those categories. We focus on the merger of the NEAR and SQUARE lexical sets in New Zealand English, presenting New Zealand participants with an unmerged speaker of Australian English. Mergers-in-progress are a uniquely interesting sound change as they showcase the asymmetry between speech perception and production. Yet, we examine mergers using spontaneous phonetic imitation, which is phenomenon that is necessarily a behavior where perceptual input influences speech production. Phonetic imitation is quantified by a perceptual measure and an acoustic calculation of mergedness using a Pillai-Bartlett trace. The results from both analyses indicate spontaneous phonetic imitation is moderated by extra-linguistic factors such as the valence of assigned conditions and social bias. We also find evidence for a decrease in the degree of mergedness in post-exposure productions. Taken together, our results suggest that under the appropriate conditions New Zealanders phonetically accommodate to Australian English and that in the process of speech imitation, mergers-in-progress can, but do not consistently, become less merged.

  18. H2S, a novel gasotransmitter, involves in gastric accommodation.

    PubMed

    Xiao, Ailin; Wang, Hongjuan; Lu, Xin; Zhu, Jianchun; Huang, Di; Xu, Tonghui; Guo, Jianqiang; Liu, Chuanyong; Li, Jingxin

    2015-11-04

    H2S is produced mainly by two enzymes:cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE), using L-cysteine (L-Cys) as the substrate. In this study, we investigated the role of H2S in gastric accommodation using CBS(+/-) mice, immunohistochemistry, immunoblot, methylene blue assay, intragastric pressure (IGP) recording and electrical field stimulation (EFS). Mouse gastric fundus expressed H2S-generating enzymes (CBS and CSE) and generated detectable amounts of H2S. The H2S donor, NaHS or L-Cys, caused a relaxation in either gastric fundus or body. The gastric compliance was significantly increased in the presence of L-Cys (1 mM). On the contrary, AOAA, an inhibitor for CBS, largely inhibited gastric compliance. Consistently, CBS(+/-) mice shows a lower gastric compliance. However, PAG, a CSE inhibitor, had no effect on gastric compliances. L-Cys enhances the non-adrenergic, non-cholinergic (NANC) relaxation of fundus strips, but AOAA reduces the magnitude of relaxations to EFS. Notably, the expression level of CBS but not CSE protein was elevated after feeding. Consistently, the production of H2S was also increased after feeding in mice gastric fundus. In addition, AOAA largely reduced food intake and body weight in mice. Furthermore, a metabolic aberration of H2S was found in patients with functional dyspepsia (FD). In conclusion, endogenous H2S, a novel gasotransmitter, involves in gastric accommodation.

  19. Right Ventricular Anatomy Can Accommodate Multiple Micra Transcatheter Pacemakers

    PubMed Central

    EGGEN, MICHAEL D.; BONNER, MATTHEW D.; IAIZZO, PAUL A.; WIKA, KENT

    2016-01-01

    Background The introduction of transcatheter pacemaker technology has the potential to significantly reduce if not eliminate a number of complications associated with a traditional leaded pacing system. However, this technology raises new questions regarding how to manage the device at end of service, the number of devices the right ventricle (RV) can accommodate, and what patient age is appropriate for this therapy. In this study, six human cadaver hearts and one reanimated human heart (not deemed viable for transplant) were each implanted with three Micra devices in traditional pacing locations via fluoroscopic imaging. Methods A total of six human cadaver hearts were obtained from the University of Minnesota Anatomy Bequest Program; the seventh heart was a heart not deemed viable for transplant obtained from LifeSource and then reanimated using Visible Heart® methodologies. Each heart was implanted with multiple Micras using imaging and proper delivery tools; in these, the right ventricular volumes were measured and recorded. The hearts were subsequently dissected to view the right ventricular anatomies and the positions and spacing between devices. Results Multiple Micra devices could be placed in each heart in traditional, clinically accepted pacing implant locations within the RV and in each case without physical device interactions. This was true even in a human heart considered to be relatively small. Conclusions Although this technology is new, it was demonstrated here that within the human heart's RV, three Micra devices could be accommodated within traditional pacing locations: with the potential in some, for even more. PMID:26710918

  20. Myopic astigmatism a substitute for accommodation in pseudophakia.

    PubMed

    Huber, C

    1981-12-16

    The power of an intraocular lens can be calculated before surgery to make the eye emmetropic or ametropic. The physiological mechanism of accommodation however, cannot be restored with an inelastic lens. An increased depth of focus in the implanted eye can be predicted through optical principles alone, if the postoperative ametropia of the implanted eye is a simple myopic astigmatism. This increased depth of focus without accommodation was tested in artificial ametropia and found to be used in nature by the seal. To increase the precision of intraocular lens calculation the average change in corneal power induced at surgery is used to predict the postoperative corneal power. By controlled suture release in the postoperative phase, the amount of induced corneal astigmatism is adjusted to obtain a simple myopic astigmatism. Patients with an intraocular lens and a simple myopic astigmatism as a residual ametropia, are spectacle independent most of the time. They need their glasses only for driving or prolonged reading. The methods used to calculate the postoperative cornea, the postoperative anterior chamber depth and the intraocular lens are described with the corresponding calculator programs for the HP 41C calculator. Clinical results and measurements of the depth of focus are shown in a series of 50 successive implant cases.

  1. Can mergers-in-progress be unmerged in speech accommodation?

    PubMed Central

    Babel, Molly; McAuliffe, Michael; Haber, Graham

    2013-01-01

    This study examines spontaneous phonetic accommodation of a dialect with distinct categories by speakers who are in the process of merging those categories. We focus on the merger of the NEAR and SQUARE lexical sets in New Zealand English, presenting New Zealand participants with an unmerged speaker of Australian English. Mergers-in-progress are a uniquely interesting sound change as they showcase the asymmetry between speech perception and production. Yet, we examine mergers using spontaneous phonetic imitation, which is phenomenon that is necessarily a behavior where perceptual input influences speech production. Phonetic imitation is quantified by a perceptual measure and an acoustic calculation of mergedness using a Pillai-Bartlett trace. The results from both analyses indicate spontaneous phonetic imitation is moderated by extra-linguistic factors such as the valence of assigned conditions and social bias. We also find evidence for a decrease in the degree of mergedness in post-exposure productions. Taken together, our results suggest that under the appropriate conditions New Zealanders phonetically accommodate to Australian English and that in the process of speech imitation, mergers-in-progress can, but do not consistently, become less merged. PMID:24069011

  2. Dividing Fractions: A Pedagogical Technique

    ERIC Educational Resources Information Center

    Lewis, Robert

    2016-01-01

    When dividing one fraction by a second fraction, invert, that is, flip the second fraction, then multiply it by the first fraction. To multiply fractions, simply multiply across the denominators, and multiply across the numerators to get the resultant fraction. So by inverting the division of fractions it is turned into an easy multiplication of…

  3. Chrysin induces G1 phase cell cycle arrest in C6 glioma cells through inducing p21Waf1/Cip1 expression: involvement of p38 mitogen-activated protein kinase.

    PubMed

    Weng, Meng-Shih; Ho, Yuan-Soon; Lin, Jen-Kun

    2005-06-15

    Flavonoids are a broadly distributed class of plant pigments, universally present in plants. They are strong anti-oxidants that can inhibit carcinogenesis in rodents. Chrysin (5,7-dihydroxyflavone) is a natural and biologically active compound extracted from many plants, honey, and propolis. It possesses potent anti-inflammatory, anti-oxidant properties, promotes cell death, and perturbing cell cycle progression. However, the mechanism by which chrysin inhibits cancer cell growth remains poorly understood. Therefore, we developed an interest in the relationship between MAPK signaling pathways and cell growth inhibition after chrysin treatment in rat C6 glioma cells. Cell viability assay and flow cytometric analysis suggested that chrysin exhibited a dose-dependent and time-dependent ability to block rat C6 glioma cell line cell cycle progression at the G1 phase. Western blotting analysis showed that the levels of Rb phosphorylation in C6 glioma cells exposed to 30 microM chrysin for 24h decreased significantly. We demonstrated the expression of cyclin-dependent kinase inhibitor, p21(Waf1/Cip1), to be significantly increased, but the p53 protein level did not change in chrysin-treated cells. Both cyclin-dependent kinase 2 (CDK2) and 4 (CDK4) kinase activities were reduced by chrysin in a dose-dependent manner. Furthermore, chrysin also inhibited proteasome activity. We further showed that chrysin induced p38-MAPK activation, and using a specific p38-MAPK inhibitor, SB203580, attenuated chrysin-induced p21(Waf1/Cip1) expression. These results suggest that chrysin exerts its growth-inhibitory effects either through activating p38-MAPK leading to the accumulation of p21(Waf1/Cip1) protein or mediating the inhibition of proteasome activity.

  4. Alterations in TP53, cyclin D2, c-Myc, p21WAF1/CIP1 and p27KIP1 expression associated with progression in B-CLL.

    PubMed

    Halina, Antosz; Artur, Paterski; Barbara, Marzec-Kotarska; Joanna, Sajewicz; Anna, Dmoszyńska

    2010-12-01

    B-cell chronic lymphocytic leukaemia (B-CLL) originates from B lymphocytes that may differ in the activation level, maturation state or cellular subgroups in peripheral blood. Tumour progression in CLL B cells seems to result in gradual accumulation of the clone of resting B lymphocytes in the early phases (G0/G1) of the cell cycle. The G1 phase is impaired in B-CLL. We investigated the gene expression of five key cell cycle regulators: TP 53, c-Myc, cyclin D2, p21WAF1/CIP1 and p27KIP1, which primarily regulate the G1 phase of the cell cycle, or S-phase entry and ultimately control the proliferation and cell growth as well as their role in B-CLL progression. The study was conducted in peripheral blood CLL lymphocytes of 40 previously untreated patients. Statistical analysis of correlations of TP53, cyclin D2, c-Myc, p21WAF1/CIP1 and p27KIP1 expressions in B-CLL patients with different Rai stages demonstrated that the progression of disease was accompanied by increases in p53, cyclin D2 and c-Myc mRNA expression. The expression of p27KIP1 was nearly statistically significant whereas that of p21 WAF1/CIP1 showed no such correlation. Moreover, high expression levels of TP53 and c-Myc genes were found to be closely associated with more aggressive forms of the disease requiring earlier therapy.

  5. Initiation and termination of DNA replication during S phase in relation to cyclins D1, E and A, p21WAF1, Cdt1 and the p12 subunit of DNA polymerase δ revealed in individual cells by cytometry.

    PubMed

    Darzynkiewicz, Zbigniew; Zhao, Hong; Zhang, Sufang; Lee, Marietta Y W T; Lee, Ernest Y C; Zhang, Zhongtao

    2015-05-20

    During our recent studies on mechanism of the regulation of human DNA polymerase δ in preparation for DNA replication or repair, multiparameter imaging cytometry as exemplified by laser scanning cytometry (LSC) has been used to assess changes in expression of the following nuclear proteins associated with initiation of DNA replication: cyclin A, PCNA, Ki-67, p21(WAF1), DNA replication factor Cdt1 and the smallest subunit of DNA polymerase δ, p12. In the present review, rather than focusing on Pol δ, we emphasize the application of LSC in these studies and outline possibilities offered by the concurrent differential analysis of DNA replication in conjunction with expression of the nuclear proteins. A more extensive analysis of the data on a correlation between rates of EdU incorporation, likely reporting DNA replication, and expression of these proteins, is presently provided. New data, specifically on the expression of cyclin D1 and cyclin E with respect to EdU incorporation as well as on a relationship between expression of cyclin A vs. p21(WAF1) and Ki-67 vs. Cdt1, are also reported. Of particular interest is the observation that this approach makes it possible to assess the temporal sequence of degradation of cyclin D1, p21(WAF1), Cdt1 and p12, each with respect to initiation of DNA replication and with respect to each other. Also the sequence or reappearance of these proteins in G2 after termination of DNA replication is assessed. The reviewed data provide a more comprehensive presentation of potential markers, whose presence or absence marks the DNA replicating cells. Discussed is also usefulness of these markers as indicators of proliferative activity in cancer tissues that may bear information on tumor progression and have a prognostic value.

  6. Accommodation with higher-order monochromatic aberrations corrected with adaptive optics

    NASA Astrophysics Data System (ADS)

    Chen, Li; Kruger, Philip B.; Hofer, Heidi; Singer, Ben; Williams, David R.

    2006-01-01

    Higher-order monochromatic aberrations in the human eye cause a difference in the appearance of stimuli at distances nearer and farther from best focus that could serve as a signed error signal for accommodation. We explored whether higher-order monochromatic aberrations affect the accommodative response to 0.5 D step changes in vergence in experiments in which these aberrations were either present as they normally are or removed with adaptive optics. Of six subjects, one could not accommodate at all for steps in either condition. One subject clearly required higher-order aberrations to accommodate at all. The remaining four subjects could accommodate in the correct direction even when higher-order aberrations were removed. No subjects improved their accommodation when higher-order aberrations were corrected, indicating that the corresponding decrease in the depth of field of the eye did not improve the accommodative response. These results are consistent with previous findings of large individual differences in the ability to accommodate in impoverished conditions. These results suggest that at least some subjects can use monochromatic higher-order aberrations to guide accommodation. They also show that some subjects can accommodate correctly when higher-order monochromatic aberrations as well as established cues to accommodation are greatly reduced.

  7. Parental accommodation of child anxiety and related symptoms: range, impact, and correlates.

    PubMed

    Thompson-Hollands, Johanna; Kerns, Caroline E; Pincus, Donna B; Comer, Jonathan S

    2014-12-01

    Parental accommodation--i.e., changes in parents' behavior in attempts to prevent or reduce child distress--has been most studied in relation to OCD. Although recent work suggests parents of children with non-OCD anxiety diagnoses also engage in accommodation, little is known about the specific forms, correlates, and associated interference of such accommodation. The present study examined the range and associated interference of parental accommodation behaviors using the newly developed Family Accommodation Checklist and Interference Scale (FACLIS) in a sample of the parents of 71 clinic-referred children with anxiety disorders (NMothers-68; NFathers-51). The FACLIS demonstrated good reliability and validity. Ninety-seven percent of mothers and 88% of fathers reported engaging in at least one type of accommodation in the previous two weeks, with parents reporting an average of roughly 4 interfering parental accommodation behaviors. Greater parental accommodation and associated interference were associated with higher maternal distress. Among the anxiety disorders, accommodation was most strongly associated with generalized and separation anxiety disorder, as well as specific phobias. Findings (a) offer psychometric support for the FACLIS as a reliable and valid tool for the assessment of accommodation range and impact, and (b) help clarify the considerable scope and interference associated with parental accommodation of childhood anxiety.

  8. The effects of a convex rear-view mirror on ocular accommodative responses.

    PubMed

    Nagata, Tatsuo; Iwasaki, Tsuneto; Kondo, Hiroyuki; Tawara, Akihiko

    2013-11-01

    Convex mirrors are universally used as rear-view mirrors in automobiles. However, the ocular accommodative responses during the use of these mirrors have not yet been examined. This study investigated the effects of a convex mirror on the ocular accommodative systems. Seven young adults with normal visual functions were ordered to binocularly watch an object in a convex or plane mirror. The accommodative responses were measured with an infrared optometer. The average of the accommodation of all subjects while viewing the object in the convex mirror were significantly nearer than in the plane mirror, although all subjects perceived the position of the object in the convex mirror as being farther away. Moreover, the fluctuations of accommodation were significantly larger for the convex mirror. The convex mirror caused the 'false recognition of distance', which induced the large accommodative fluctuations and blurred vision. Manufactures should consider the ocular accommodative responses as a new indicator for increasing automotive safety.

  9. Probabilistic robust stabilization of fractional order systems with interval uncertainty.

    PubMed

    Alagoz, Baris Baykant; Yeroglu, Celaleddin; Senol, Bilal; Ates, Abdullah

    2015-07-01

    This study investigates effects of fractional order perturbation on the robust stability of linear time invariant systems with interval uncertainty. For this propose, a probabilistic stability analysis method based on characteristic root region accommodation in the first Riemann sheet is developed for interval systems. Stability probability distribution is calculated with respect to value of fractional order. Thus, we can figure out the fractional order interval, which makes the system robust stable. Moreover, the dependence of robust stability on the fractional order perturbation is analyzed by calculating the order sensitivity of characteristic polynomials. This probabilistic approach is also used to develop a robust stabilization algorithm based on parametric perturbation strategy. We present numerical examples demonstrating utilization of stability probability distribution in robust stabilization problems of interval uncertain systems.

  10. Sensory outcome with nonsurgical management of esotropia with convergence excess (a high accommodative convergence/accommodation ratio).

    PubMed

    Pratt-Johnson, J A; Tillson, G

    1984-08-01

    Twenty-seven patients with esotropia and convergence excess (a high accommodative convergence/accommodation [AC/A] ratio) managed nonsurgically underwent a final standardized evaluation of their sensory and motor status after a follow-up period of at least 8 years. All had a deviation with distance fixation of less than 10 prism dioptres (PD) of esotropia with full optical correction both initially and throughout the follow-up period. The average spherical-equivalent refractive error was +2.3 D. The AC/A ratio had tended to decrease with age, and most patients had fusion, although only a small proportion had central fusion and stereopsis. Approximately half of the patients had been treated with bifocals, but their sensory outcome did not differ from that of the other patients. Miotics had not been used for more than a few months in any patient, as they were ineffective in reducing the deviation with near fixation to less than 10 PD of esotropia. A study, possibly a multicentre one, involving larger numbers of patients should be designed to find out whether bifocal therapy offers an advantage in the final sensory outcome of such patients.

  11. FRACTIONAL PEARSON DIFFUSIONS.

    PubMed

    Leonenko, Nikolai N; Meerschaert, Mark M; Sikorskii, Alla

    2013-07-15

    Pearson diffusions are governed by diffusion equations with polynomial coefficients. Fractional Pearson diffusions are governed by the corresponding time-fractional diffusion equation. They are useful for modeling sub-diffusive phenomena, caused by particle sticking and trapping. This paper provides explicit strong solutions for fractional Pearson diffusions, using spectral methods. It also presents stochastic solutions, using a non-Markovian inverse stable time change.

  12. FRACTIONAL PEARSON DIFFUSIONS

    PubMed Central

    Leonenko, Nikolai N.; Meerschaert, Mark M.

    2013-01-01

    Pearson diffusions are governed by diffusion equations with polynomial coefficients. Fractional Pearson diffusions are governed by the corresponding time-fractional diffusion equation. They are useful for modeling sub-diffusive phenomena, caused by particle sticking and trapping. This paper provides explicit strong solutions for fractional Pearson diffusions, using spectral methods. It also presents stochastic solutions, using a non-Markovian inverse stable time change. PMID:23626377

  13. An Integrated Approach to Damage Accommodation in Flight Control

    NASA Technical Reports Server (NTRS)

    Boskovic, Jovan D.; Knoebel, Nathan; Mehra, Raman K.; Gregory, Irene

    2008-01-01

    In this paper we present an integrated approach to in-flight damage accommodation in flight control. The approach is based on Multiple Models, Switching and Tuning (MMST), and consists of three steps: In the first step the main objective is to acquire a realistic aircraft damage model. Modeling of in-flight damage is a highly complex problem since there is a large number of issues that need to be addressed. One of the most important one is that there is strong coupling between structural dynamics, aerodynamics, and flight control. These effects cannot be studied separately due to this coupling. Once a realistic damage model is available, in the second step a large number of models corresponding to different damage cases are generated. One possibility is to generate many linear models and interpolate between them to cover a large portion of the flight envelope. Once these models have been generated, we will implement a recently developed-Model Set Reduction (MSR) technique. The technique is based on parameterizing damage in terms of uncertain parameters, and uses concepts from robust control theory to arrive at a small number of "centered" models such that the controllers corresponding to these models assure desired stability and robustness properties over a subset in the parametric space. By devising a suitable model placement strategy, the entire parametric set is covered with a relatively small number of models and controllers. The third step consists of designing a Multiple Models, Switching and Tuning (MMST) strategy for estimating the current operating regime (damage case) of the aircraft, and switching to the corresponding controller to achieve effective damage accommodation and the desired performance. In the paper present a comprehensive approach to damage accommodation using Model Set Design,MMST, and Variable Structure compensation for coupling nonlinearities. The approach was evaluated on a model of F/A-18 aircraft dynamics under control effector damage

  14. Space Station life sciences guidelines for nonhuman experiment accommodation

    NASA Technical Reports Server (NTRS)

    Arno, R.; Hilchey, J.

    1985-01-01

    Life scientists will utilize one of four habitable modules which constitute the initial Space Station configuration. This module will be initially employed for studies related to nonhuman and human life sciences. At a later date, a new module, devoted entirely to nonhuman life sciences will be launched. This report presents a description of the characteristics of a Space Station laboratory facility from the standpoint of nonhuman research requirements. Attention is given to the science rationale for experiments which support applied medical research and basic gravitational biology, mission profiles and typical equipment and subsystem descriptions, issues associated with the accommodation of nonhuman life sciences on the Space Station, and conceptual designs for the initial operational capability configuration and later Space Station life-sciences research facilities.

  15. Baudoinia, a new genus to accommodate Torula compniacensis.

    PubMed

    Scott, James A; Untereiner, Wendy A; Ewaze, Juliet O; Wong, Bess; Doyle, David

    2007-01-01

    Baudoinia gen. nov. is described to accommodate Torula compniacensis. Reported originally from the walls of buildings near brandy maturation warehouses in Cognac, France, species of Baudoinia are cosmopolitan colonists of exposed surfaces subjected to large diurnal temperature shifts, episodic high relative humidity and wetting, and ambient airborne ethanol. Morphologically B. compniacensis resembles some anamorphic Mycosphaerellaceae in possessing dark brown, nonseptate or uniseptate conidia with coarsely roughened walls that are borne acropetally in unbranched chains and released by schizolytic dehiscence. Analysis of partial nuclear rDNA SSU sequences positions B. compniacensis in the order Capnodiales and reveals that it is most closely related to the microcolonial genus Friedmanniomyces. Heat resistance is induced by brief sublethal temperature exposure.

  16. Disturbance Accommodating Adaptive Control with Application to Wind Turbines

    NASA Technical Reports Server (NTRS)

    Frost, Susan

    2012-01-01

    Adaptive control techniques are well suited to applications that have unknown modeling parameters and poorly known operating conditions. Many physical systems experience external disturbances that are persistent or continually recurring. Flexible structures and systems with compliance between components often form a class of systems that fail to meet standard requirements for adaptive control. For these classes of systems, a residual mode filter can restore the ability of the adaptive controller to perform in a stable manner. New theory will be presented that enables adaptive control with accommodation of persistent disturbances using residual mode filters. After a short introduction to some of the control challenges of large utility-scale wind turbines, this theory will be applied to a high-fidelity simulation of a wind turbine.

  17. Automation for Accommodating Fuel-Efficient Descents in Constrained Airspace

    NASA Technical Reports Server (NTRS)

    Coopenbarger, Richard A.

    2010-01-01

    Continuous descents at low engine power are desired to reduce fuel consumption, emissions and noise during arrival operations. The challenge is to allow airplanes to fly these types of efficient descents without interruption during busy traffic conditions. During busy conditions today, airplanes are commonly forced to fly inefficient, step-down descents as airtraffic controllers work to ensure separation and maximize throughput. NASA in collaboration with government and industry partners is developing new automation to help controllers accommodate continuous descents in the presence of complex traffic and airspace constraints. This automation relies on accurate trajectory predictions to compute strategic maneuver advisories. The talk will describe the concept behind this new automation and provide an overview of the simulations and flight testing used to develop and refine its underlying technology.

  18. Communication accommodation and managing musculoskeletal disorders: doctors' and patients' perspectives.

    PubMed

    Baker, Susan C; Gallois, Cindy; Driedger, S Michelle; Santesso, Nancy

    2011-06-01

    This study examined the ways in which health care providers (general practitioners and specialists) and patients communicate with each other about managing musculoskeletal (MSK) disorders, a major cause of long-term pain and physical disability. In managing their illness, patients must interact closely with health care providers, who play a large role in transferring knowledge to them. In-depth interviews with patients, general practitioners, and specialist rheumatologists in Australia and Canada were analyzed using Leximancer (a text-mining tool). Results indicated that, in their communication, doctors subtly emphasized accepting and adjusting to the illness ("new normal"), whereas patients emphasized pain relief and getting "back to normal." These results suggest that doctors and patients should accommodate in their communication across subtle and often unexpressed differences in the priorities of provider and patient, or they are likely to be at cross purposes and thus less effective.

  19. Crude oil exposure results in oxidative stress-mediated dysfunctional development and reproduction in the copepod Tigriopus japonicus and modulates expression of cytochrome P450 (CYP) genes.

    PubMed

    Han, Jeonghoon; Won, Eun-Ji; Hwang, Dae-Sik; Shin, Kyung-Hoon; Lee, Yong Sung; Leung, Kenneth Mei-Yee; Lee, Su-Jae; Lee, Jae-Seong

    2014-07-01

    In this study, we investigated the effects of the water-accommodated fraction (WAF) of crude oil on the development and reproduction of the intertidal copepod Tigriopus japonicus through life-cycle experiments. Furthermore, we investigated the mechanisms underlying the toxic effects of WAF on this benthic organism by studying expression patterns of cytochrome P450 (CYP) genes. Development of T. japonicus was delayed and molting was interrupted in response to WAF exposure. Hatching rate was also significantly reduced in response to WAF exposure. Activities of antioxidant enzymes such as glutathione S-transferase (GST), glutathione reductase (GR), and catalase (CAT) were increased by WAF exposure in a concentration-dependent manner. These results indicated that WAF exposure resulted in oxidative stress, which in turn was associated with dysfunctional development and reproduction. To evaluate the involvement of cytochrome P450 (CYP) genes, we cloned the entire repertoire of CYP genes in T. japonicus (n=52) and found that the CYP genes belonged to five different clans (i.e., Clans 2, 3, 4, mitochondrial, and 20). We then examined expression patterns of these 52 CYP genes in response to WAF exposure. Three TJ-CYP genes (CYP3024A2, CYP3024A3, and CYP3027C2) belonging to CYP clan 3 were significantly induced by WAF exposure in a time- and concentration-dependent manner. We identified aryl hydrocarbon responsive elements (AhRE), xenobiotic responsive elements (XREs), and metal response elements (MRE) in the promoter regions of these three CYP genes, suggesting that these genes are involved in detoxification of toxicants. Overall, our results indicate that WAF can trigger oxidative stress and thus induce dysfunctional development and reproduction in the copepod T. japonicus. Furthermore, we identified three TJ-CYP genes that represent potential biomarkers of oil pollution.

  20. Force production and mechanical accommodation during convergent extension

    PubMed Central

    Zhou, Jian; Pal, Siladitya; Maiti, Spandan; Davidson, Lance A.

    2015-01-01

    Forces generated within the embryo during convergent extension (CE) must overcome mechanical resistance to push the head away from the rear. As mechanical resistance increases more than eightfold during CE and can vary twofold from individual to individual, we have proposed that developmental programs must include mechanical accommodation in order to maintain robust morphogenesis. To test this idea and investigate the processes that generate forces within early embryos, we developed a novel gel-based sensor to report force production as a tissue changes shape; we find that the mean stress produced by CE is 5.0±1.6 Pascal (Pa). Experiments with the gel-based force sensor resulted in three findings. (1) Force production and mechanical resistance can be coupled through myosin contractility. The coupling of these processes can be hidden unless affected tissues are challenged by physical constraints. (2) CE is mechanically adaptive; dorsal tissues can increase force production up to threefold to overcome a stiffer microenvironment. These findings demonstrate that mechanical accommodation can ensure robust morphogenetic movements against environmental and genetic variation that might otherwise perturb development and growth. (3) Force production is distributed between neural and mesodermal tissues in the dorsal isolate, and the notochord, a central structure involved in patterning vertebrate morphogenesis, is not required for force production during late gastrulation and early neurulation. Our findings suggest that genetic factors that coordinately alter force production and mechanical resistance are common during morphogenesis, and that their cryptic roles can be revealed when tissues are challenged by controlled biophysical constraints. PMID:25670794

  1. Can Kindergartners Do Fractions?

    ERIC Educational Resources Information Center

    Cwikla, Julie

    2014-01-01

    Mathematics professor Julie Cwikla decided that she needed to investigate young children's understandings and see what precurricular partitioning notions young minds bring to the fraction table. Cwikla realized that only a handful of studies have examined how preschool-age and early elementary school-age students solve fraction problems (Empson…

  2. An Appetite for Fractions

    ERIC Educational Resources Information Center

    Wilkerson, Trena L.; Bryan, Tommy; Curry, Jane

    2012-01-01

    This article describes how using candy bars as models gives sixth-grade students a taste for learning to represent fractions whose denominators are factors of twelve. Using paper models of the candy bars, students explored and compared fractions. They noticed fewer different representations for one-third than for one-half. The authors conclude…

  3. On fractional programming

    SciTech Connect

    Bajona-Xandri, C.; Martinez-Legaz, J.E.

    1994-12-31

    This paper studies the minimax fractional programming problem, assuming quasiconvexity of the objective function, under the lower subdifferentiability viewpoint. Necessary and sufficient optimality conditions and dual properties are found. We present applications of this theory to find the Pareto efficient solutions of a multiobjective fractional problem and to solve several economic models.

  4. (Carbon isotope fractionation inplants)

    SciTech Connect

    O'Leary, M.H.

    1990-01-01

    The objectives of this research are: To develop a theoretical and experimental framework for understanding isotope fractionations in plants; and to develop methods for using this isotope fractionation for understanding the dynamics of CO{sub 2} fixation in plants. Progress is described.

  5. Fractional dissipative standard map.

    PubMed

    Tarasov, Vasily E; Edelman, M

    2010-06-01

    Using kicked differential equations of motion with derivatives of noninteger orders, we obtain generalizations of the dissipative standard map. The main property of these generalized maps, which are called fractional maps, is long-term memory. The memory effect in the fractional maps means that their present state of evolution depends on all past states with special forms of weights. Already a small deviation of the order of derivative from the integer value corresponding to the regular dissipative standard map (small memory effects) leads to the qualitatively new behavior of the corresponding attractors. The fractional dissipative standard maps are used to demonstrate a new type of fractional attractors in the wide range of the fractional orders of derivatives.

  6. The Rate of Change of Vergence-Accommodation Conflict Affects Visual Discomfort

    PubMed Central

    Kane, David; Banks, Martin S.

    2014-01-01

    Stereoscopic (S3D) displays create conflicts between the distance to which the eyes must converge and the distance to which the eyes must accommodate. Such conflicts require the viewer to overcome the normal coupling between vergence and accommodation, and this effort appears to cause viewer discomfort. Vergence-accommodation coupling is driven by the phasic components of the underlying control systems, and those components respond to relatively fast changes in vergence and accommodative stimuli. Given the relationship between phasic changes and vergence-accommodation coupling, we examined how the rate of change in the vergence-accommodation conflict affects viewer discomfort. We used a stereoscopic display that allows independent manipulation of the stimuli to vergence and accommodation. We presented stimuli that simulate natural viewing (i.e., vergence and accommodative stimuli changed together) and stimuli that simulate S3D viewing (i.e., vergence stimulus changes but accommodative stimulus remains fixed). The changes occurred at 0.01, 0.05, or 0.25Hz. The lowest rate is too slow to stimulate the phasic components while the highest rate is well within the phasic range. The results were consistent with our expectation: somewhat greater discomfort was experienced when stimulus distance changed rapidly, particularly in S3D viewing when the vergence stimulus changed but the accommodative stimulus did not. These results may help in the generation of guidelines for the creation and viewing of stereo content with acceptable viewer comfort. PMID:25448713

  7. Effects of linguistic complexity and accommodations on estimates of ability for students with learning disabilities.

    PubMed

    Cawthon, Stephanie W; Kaye, Alyssa D; Lockhart, L Leland; Beretvas, S Natasha

    2012-06-01

    Many students with learning disabilities (SLD) participate in standardized assessments using test accommodations such as extended time, having the test items read aloud, or taking the test in a separate setting. Yet there are also aspects of the test items themselves, particularly the language demand, which may contribute to the effects of test accommodations. This study entailed an analysis of linguistic complexity (LC) and accommodation use for SLD in grade four on 2005 National Assessment of Educational Progress (NAEP) reading and mathematics items. The purpose of this study was to investigate (a) the effects of test item LC on reading and mathematics item difficulties for SLD; (b) the impact of accommodations (presentation, response, setting, or timing) on estimates of student ability, after controlling for LC effects; and (c) the impact of differential facet functioning (DFF), a person-by-item-descriptor interaction, on estimates of student ability, after controlling for LC and accommodations' effects. For both reading and mathematics, the higher an item's LC, the more difficult it was for SLD. After controlling for differences due to accommodations, LC was not a significant predictor of mathematics items' difficulties, but it remained a significant predictor for reading items. There was no effect of accommodations on mathematics item performance, but for reading items, students who received presentation and setting accommodations scored lower than those who did not. No significant LC-by-accommodation interactions were found for either subject area, indicating that the effect of LC did not depend on the type of accommodation received.

  8. The rate of change of vergence-accommodation conflict affects visual discomfort.

    PubMed

    Kim, Joohwan; Kane, David; Banks, Martin S

    2014-12-01

    Stereoscopic (S3D) displays create conflicts between the distance to which the eyes must converge and the distance to which the eyes must accommodate. Such conflicts require the viewer to overcome the normal coupling between vergence and accommodation, and this effort appears to cause viewer discomfort. Vergence-accommodation coupling is driven by the phasic components of the underlying control systems, and those components respond to relatively fast changes in vergence and accommodative stimuli. Given the relationship between phasic changes and vergence-accommodation coupling, we examined how the rate of change in the vergence-accommodation conflict affects viewer discomfort. We used a stereoscopic display that allows independent manipulation of the stimuli to vergence and accommodation. We presented stimuli that simulate natural viewing (i.e., vergence and accommodative stimuli changed together) and stimuli that simulate S3D viewing (i.e., vergence stimulus changes but accommodative stimulus remains fixed). The changes occurred at 0.01, 0.05, or 0.25 Hz. The lowest rate is too slow to stimulate the phasic components while the highest rate is well within the phasic range. The results were consistent with our expectation: somewhat greater discomfort was experienced when stimulus distance changed rapidly, particularly in S3D viewing when the vergence stimulus changed but the accommodative stimulus did not. These results may help in the generation of guidelines for the creation and viewing of stereo content with acceptable viewer comfort.

  9. Optimal secondary coil design for inductive powering of the Artificial Accommodation System.

    PubMed

    Nagel, J A; Krug, M; Gengenbach, U; Guth, H; Bretthauer, G; Guthoff, R F

    2011-01-01

    Age-related ailments like presbyopia and cataract are increasing concerns in the aging society. Both go along with a loss of ability to accommodate. A new approach to restore the patients' ability to accommodate is the Artificial Accommodation System. This micro mechatronic system will be implanted into the capsular bag to replace the human crystalline lens. Depending on the patients' actual need for accommodation, the Artificial Accommodation System autonomously adapts the refractive power of its integrated optical element in a way that the projection on the patients' retina results in a sharp image. As the Artificial Accommodation System is an active implant, its subsystems have to be supplied with electrical energy. Evolving technologies, like energy harvesting, which can potentially be used to power an implant like the Artificial Accommodation System are at the current state of art not sufficient to power the Artificial Accommodation System autonomously [1]. In the near future, therefore an inductive power supply system will be developed which includes an energy storage to power the Artificial Accommodation System autonomously over a period of 24 h and can be recharged wirelessly. This Paper describes a new possibility to optimize the secondary coil design in a solely analytical way, based on a new figure of merit. Within this paper the developed figure of merit is applied to optimize the secondary coil design for the Artificial Accommodation System.

  10. p21(Cip-1/SDI-1/WAF-1) expression via the mitogen-activated protein kinase signaling pathway in insulin-induced chondrogenic differentiation of ATDC5 cells.

    PubMed

    Nakajima, Masahiro; Negishi, Yoichi; Tanaka, Hiroyasu; Kawashima, Kohtaro

    2004-08-06

    The embryonal carcinoma-derived cell line, ATDC5, differentiates into chondrocytes in response to insulin or insulin-like growth factor-I stimulation. In this study, we investigated the roles of mitogen-activated protein (MAP) kinases in insulin-induced chondrogenic differentiation of ATDC5 cells. Insulin-induced accumulation of glycosaminoglycan and expression of chondrogenic differentiation markers, type II collagen, type X collagen, and aggrecan mRNA were inhibited by the MEK1/2 inhibitor (U0126) and the p38 MAP kinase inhibitor (SB203580). Conversely, the JNK inhibitor (SP600125) enhanced the synthesis of glycosaminoglycan and expression of chondrogenic differentiation markers. Insulin-induced phosphorylation of ERK1/2 and JNK but not that of p38 MAP kinase. We have previously clarified that the induction of the cyclin-dependent kinase inhibitor, p21(Cip-1/SDI-1/WAF-1), is essential for chondrogenic differentiation of ATDC5 cells. To assess the relationship between the induction of p21 and MAP kinase activity, we investigated the effect of these inhibitors on insulin-induced p21 expression in ATDC5 cells. Insulin-induced accumulation of p21 mRNA and protein was inhibited by the addition of U0126 and SB203580. In contrast, SP600125 enhanced it. Inhibitory effects of U0126 or stimulatory effects of SP600125 on insulin-induced chondrogenic differentiation were observed when these inhibitors exist in the early phase of differentiation, suggesting that MEK/ERK and JNK act on early phase differentiation. SB202580, however, is necessary not only for early phase but also for late phase differentiation, indicating that p38 MAP kinase stimulates differentiation by acting during the entire period of cultivation. These results for the first time demonstrate that up-regulation of p21 expression by ERK1/2 and p38 MAP kinase is required for chondrogenesis, and that JNK acts as a suppressor of chondrogenesis by down-regulating p21 expression.

  11. SIAH-1 promotes apoptosis and tumor suppression through a network involving the regulation of protein folding, unfolding, and trafficking: Identification of common effectors with p53 and p21Waf1

    PubMed Central

    Roperch, Jean-Pierre; Lethrone, Florence; Prieur, Sylvie; Piouffre, Laurence; Israeli, David; Tuynder, Marcel; Nemani, Mona; Pasturaud, Patricia; Gendron, Marie-Claude; Dausset, Jean; Oren, Moshe; Amson, Robert B.; Telerman, Adam

    1999-01-01

    We have previously described biological model systems for studying tumor suppression in which, by using H-1 parvovirus as a selective agent, cells with a strongly suppressed malignant phenotype (KS or US) were derived from malignant cell lines (K562 or U937). By using cDNA display on the K562/KS cells, 15 cDNAs were now isolated, corresponding to genes differentially regulated in tumor suppression. Of these, TSAP9 corresponds to a TCP-1 chaperonin, TSAP13 to a regulatory proteasome subunit, and TSAP21 to syntaxin 11, a vesicular trafficking molecule. The 15 cDNAs were used as a molecular fingerprint in different tumor-suppression models. We found that a similar pattern of differential regulation is shared by activation of p53, p21Waf1, and the human homologue of Drosophila seven in absentia, SIAH-1. Because SIAH-1 is differentially expressed in the various models, we characterized it at the protein and functional levels. The 32-kDa, mainly nuclear protein encoded by SIAH-1, can induce apoptosis and promote tumor suppression. These results suggest the existence of a common mechanism of tumor suppression and apoptosis shared by p53, p21Waf1, and SIAH-1 and involving regulation of the cellular machinery responsible for protein folding, unfolding, and trafficking. PMID:10393949

  12. TSPYL5 is involved in cell growth and the resistance to radiation in A549 cells via the regulation of p21{sup WAF1/Cip1} and PTEN/AKT pathway

    SciTech Connect

    Kim, Eun Jin; Lee, So Yong; Kim, Tae Rim; Choi, Soo Im; Cho, Eun Wie; Kim, Kug Chan; Kim, In Gyu

    2010-02-12

    TSPYL5, encoding testis-specific Y-like protein, has been postulated to be a tumor suppressor gene, and its hypermethylation is often associated with human disease, especially cancer. In this study, we report that the TSPYL5 gene was less methylated (30%) in A549 lung adenocarcinoma cells, which are relatively resistant to {gamma}-radiation, than in H460 lung cancer cells, in which the TSPYL5 gene was hypermethylated (95%); thus, the expression level of TSPYL5 is much higher in A549 cells than in H460 cells. We showed that TSPYL5 suppression with silencing RNA in A549 cells up-regulated cellular PTEN, followed by down-regulation of AKT activation. Therefore, blockage of TSPYL5 sensitized A549 cells to cytotoxic agents such as {gamma}-radiation. In addition, TSPYL5 suppression also showed an increased level of p21{sup WAF1/Cip1} and subsequently induced inhibition of cell growth in A549 cells. The overexpression of TSPYL5 in H460 cells showed the opposite effects. This study provides the first demonstration that TSPYL5 modulates cell growth and sensitization of cells to the detrimental effects of damaging agents via regulation of p21{sup WAF1/Cip1} and PTEN/AKT pathway.

  13. Depth discrimination of constant angular size stimuli in action space: role of accommodation and convergence cues

    PubMed Central

    Naceri, Abdeldjallil; Moscatelli, Alessandro; Chellali, Ryad

    2015-01-01

    In our daily life experience, the angular size of an object correlates with its distance from the observer, provided that the physical size of the object remains constant. In this work, we investigated depth perception in action space (i.e., beyond the arm reach), while keeping the angular size of the target object constant. This was achieved by increasing the physical size of the target object as its distance to the observer increased. To the best of our knowledge, this is the first time that a similar protocol has been tested in action space, for distances to the observer ranging from 1.4–2.4 m. We replicated the task in virtual and real environments and we found that the performance was significantly different between the two environments. In the real environment, all participants perceived the depth of the target object precisely. Whereas, in virtual reality (VR) the responses were significantly less precise, although, still above chance level in 16 of the 20 observers. The difference in the discriminability of the stimuli was likely due to different contributions of the convergence and the accommodation cues in the two environments. The values of Weber fractions estimated in our study were compared to those reported in previous studies in peripersonal and action space. PMID:26441608

  14. Accommodation of pyrimidine dimers during replication of UV-damaged simian virus 40 DNA.

    PubMed Central

    Stacks, P C; White, J H; Dixon, K

    1983-01-01

    UV irradiation of simian virus 40-infected cells at fluences between 20 and 60 J/m2, which yield one to three pyrimidine dimers per simian virus 40 genome, leads to a fluence-dependent progressive decrease in simian virus 40 DNA replication as assayed by incorporation of [3H]deoxyribosylthymine into viral DNA. We used a variety of biochemical and biophysical techniques to show that this decrease is due to a block in the progression of replicative-intermediate molecules to completed form I molecules, with a concomitant decrease in the entry of molecules into the replicating pool. Despite this UV-induced inhibition of replication, some pyrimidine dimer-containing molecules become fully replicated after UV irradiation. The fraction of completed molecules containing dimers goes up with time such that by 3 h after a UV fluence of 40 J/m2, more than 50% of completed molecules contain pyrimidine dimers. We postulate that the cellular replication machinery can accommodate limited amounts of UV-induced damage and that the progressive decrease in simian virus 40 DNA synthesis after UV irradiation is due to the accumulation in the replication pool of blocked molecules containing levels of damage greater than that which can be tolerated. PMID:6621531

  15. Drag and energy accommodation coefficients during sunspot maximum

    NASA Astrophysics Data System (ADS)

    Pardini, Carmen; Anselmo, Luciano; Moe, Kenneth; Moe, Mildred M.

    A hundred years of laboratory measurements have shown that gas-surface interactions depend not only on the chemistry and energy of the incident particles but also on the degree of surface contamination. The conditions appropriate to gas-surface interaction in space have not been successfully duplicated in the laboratory. Consequently, knowledge of satellite drag coefficients has been dependent upon opportunities to compare theoretical models with observations of satellite decay. From such studies it is now known that the great majority of molecules which strike satellite surfaces are reemitted in a diffuse angular distribution with an energy loss given by the energy accommodation coefficient, α. Although a few measurements of α were made in the past, none was made near sunspot maximum. In the present study, we take advantage of the increasing data base to compare theoretical determinations of satellite drag coefficients with the history of satellite orbital decay during sunspot maximum. An example is the SNOE satellite which was in a circular orbit with an initial perigee altitude of 515 km during dates from October 1999 to December 2002. SNOE had a cylinder-like shape with a hexagonal cross section. It was attitude stabilized so that it maintained a constant aspect relative to the incident velocity vector, a feature which facilitated the computation of its drag coefficient as a function of α. The satellite drag coefficient was obtained by fitting, in a least squares sense, the semi-major axis decay inferred from the historical two-line elements acquired by the US Space Surveillance Network. All the principal orbital perturbations, namely geopotential harmonics up to the 16th degree and order, third body attraction of the Moon and the Sun, direct solar radiation pressure (with eclipses), and aerodynamic drag were included, using the Jacchia Bowman 2006 (JB2006) model to describe the atmospheric density. The average drag coefficient (fitted to JB2006), calculated

  16. Fractional calculus in bioengineering.

    PubMed

    Magin, Richard L

    2004-01-01

    Fractional calculus (integral and differential operations of noninteger order) is not often used to model biological systems. Although the basic mathematical ideas were developed long ago by the mathematicians Leibniz (1695), Liouville (1834), Riemann (1892), and others and brought to the attention of the engineering world by Oliver Heaviside in the 1890s, it was not until 1974 that the first book on the topic was published by Oldham and Spanier. Recent monographs and symposia proceedings have highlighted the application of fractional calculus in physics, continuum mechanics, signal processing, and electromagnetics, but with few examples of applications in bioengineering. This is surprising because the methods of fractional calculus, when defined as a Laplace or Fourier convolution product, are suitable for solving many problems in biomedical research. For example, early studies by Cole (1933) and Hodgkin (1946) of the electrical properties of nerve cell membranes and the propagation of electrical signals are well characterized by differential equations of fractional order. The solution involves a generalization of the exponential function to the Mittag-Leffler function, which provides a better fit to the observed cell membrane data. A parallel application of fractional derivatives to viscoelastic materials establishes, in a natural way, hereditary integrals and the power law (Nutting/Scott Blair) stress-strain relationship for modeling biomaterials. In this review, I will introduce the idea of fractional operations by following the original approach of Heaviside, demonstrate the basic operations of fractional calculus on well-behaved functions (step, ramp, pulse, sinusoid) of engineering interest, and give specific examples from electrochemistry, physics, bioengineering, and biophysics. The fractional derivative accurately describes natural phenomena that occur in such common engineering problems as heat transfer, electrode/electrolyte behavior, and sub

  17. The effects of attribution of responsibility and work history on perceptions of reasonable accommodations.

    PubMed

    Mitchell, Tara L; Kovera, Margaret Bull

    2006-12-01

    Under the Americans with Disabilities Act (ADA), employers must provide employees with disabilities reasonable accommodations that will enable them to perform job duties, as long as the accommodations do not financially burden the organization. Two studies were conducted to investigate whether disability origin and/or prior work history impermissibly influence the granting of reasonable accommodations under the ADA. In both studies, participants granted more accommodations for employees whose disability was caused by some external factor than for those whose disability was caused by the employee's own behavior. In Study Two, participants also granted more and costlier accommodations for an employee with an excellent work history than for an employee with an average work history. Implications of the use of extralegal factors in accommodation decisions are discussed.

  18. Requirement for measurement of accommodation response based image blur due to the integral photography

    NASA Astrophysics Data System (ADS)

    Yano, Sumio; Imai, Hiromichi; Park, Min-Chul

    2016-06-01

    In the first part of this paper, the principle and the development of IP display using computer software were described. Next, the measurement results of accommodation response for the developed IP display were described. As a result, the accommodation response was linearly changed as the depth position of the visual target moved in and out of the range of the depth of focus. On the other hand, the influences generated by the image blur for the accommodation response were investigated experimentally using stereoscopic images. The results showed that the accommodation response was coincident to the convergence point of stereoscopic images with less than 3 cpd spatial resolution. Based on these results, the considerations of the measurement results of the accommodation response for the development IP were examined. The requirements of the measurement condition of accommodation response for IP were also discussed.

  19. A slide rule for calculating the ocular accommodation of an ametrope corrected with a spectacle lens.

    PubMed

    Wang, Guang-Ji

    2007-07-01

    A slide rule has been designed to calculate the ocular accommodation of an ametrope corrected with a spectacle lens. The slide rule makes the calculation itself easier to perform than with traditional methods and is easily applicable in a clinical setting. In the slide rule, there are 3 scales indicating the power of the spectacle lens, the viewing distance, and the ocular accommodation. The most accurate accommodative unit was used to design the slide rule. The ocular accommodation is the product of the accommodative unit and the dioptric viewing distance. The calculating results are accurate from +21 diopters to all minus powers of the spectacle lens. In a clinical setting, the patients can be advised how much accommodation they exert before and after the refractive surgeries.

  20. The Dark Focus of Visual Accommodation: Its Existence, Its Measurement, Its Effects

    DTIC Science & Technology

    1979-11-01

    underestimated. 91 11 £ (I, 1 120 REFERENCES Allen, M. The stimulus to accommodation. American Journal of Optometry and Physiological 0 , 1955 , U2, 79-84. Baldwin ...the time men turned to written recoras to preserve their accumulated knowledge. Indeed, seeing has inspired countless diverse poetry and prose and...state. v Allen ( 1955 ), investigating the stimulus to accommodation, found fluctuations in accommodation that were not reflected in convergence V