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Sample records for accumulated fatigue damage

  1. Fatigue damage accumulation in nickel prior to crack initiation

    NASA Technical Reports Server (NTRS)

    Grobstein, T. L.; Sivashankaran, S.; Welsch, G.; Panigrahi, N.; Mcgervey, J. D.; Blue, J. W.

    1991-01-01

    The accumulation of lattice defects during fatigue cycling of nickel was investigated by electrical resistivity measurements, positron annihilation lifetime spectroscopy and transmission electron microscopy. Dislocations and vacancy clusters were found to be the main defect types. During cycling of axial and flexural samples at constant load amplitude, the dislocations form a saturated structure early in the fatigue life. This saturated structure consists of a cellular dislocation matrix, in which persistent slip bands (PSBs) begin to operate after the saturation has been established. Vacancies and vacancy clusters are formed during fatigue as a consequence of repetitive dislocation glide in the PSB structure. When PSBs operate, the matrix is assumed to be dormant, allowing vacancies to accumulate preferentially in the PSBs. The increase in vacancy concentration then accounts for the monotonic accumulation of fatigue damage, which points to the importance of vacancy accumulation as a precursor to crack nucleation.

  2. Model of flexural fatigue damage accumulation for cortical bone.

    PubMed

    Griffin, L V; Gibeling, J C; Martin, R B; Gibson, V A; Stover, S M

    1997-07-01

    Analytical models that predict modulus degradation in cortical bone subjected to uniaxial fatigue loading in tension and compression are presented. On the basis of experimental observations, damage was modeled as self-limiting for tension but not for compression. These mechanistic uniaxial damage models were then developed into a model for flexural fatigue of cortical bone based on laminated beam theory. The unknown coefficients in the uniaxial damage models were obtained by successfully fitting the resulting equations to uniaxial fatigue data from the literature on human cortical bone in tension and compression. Then, the predictions of the flexural model for the behavior of human cortical bone were compared with experimental results from a small but independent set of specimens tested at three different ranges of load in our laboratory. The behavior of the modulus degradation curves and the flexural fatigue lives of the specimens were in excellent agreement with the predictions of the model.

  3. Characteristics of fatigue life and damage accumulation of short fiber-reinforced polymer composites

    SciTech Connect

    Yokobori, A.T. Jr.; Takeda, Hidetoshi; Adachi, Takeshi; Ha, J.C.; Yokobori, Takeo

    1996-12-31

    The relation between fatigue life and damage accumulation of fiber-reinforced polymer composite (FRP) is not yet clarified. For practical use of FRP, it is necessary to relate the fatigue life to the mechanism of damage accumulation. Damage formation is controlled by the mechanical behavior of the interface between the matrix and fiber. The authors used short glass fiber-reinforced polycarbonate composite in the experiments. By using an in situ (real time) observational fatigue testing machine, they investigated the relationship between fatigue life and damage accumulation. From these results, the fatigue life of this material was found to be dominated by damage accumulation which results from microfracture at the interface between the matrix and fiber. This microfracture is controlled by a cycle-dependent mechanism.

  4. A Modified Nonlinear Damage Accumulation Model for Fatigue Life Prediction Considering Load Interaction Effects

    PubMed Central

    Huang, Hong-Zhong; Yuan, Rong

    2014-01-01

    Many structures are subjected to variable amplitude loading in engineering practice. The foundation of fatigue life prediction under variable amplitude loading is how to deal with the fatigue damage accumulation. A nonlinear fatigue damage accumulation model to consider the effects of load sequences was proposed in earlier literature, but the model cannot consider the load interaction effects, and sometimes it makes a major error. A modified nonlinear damage accumulation model is proposed in this paper to account for the load interaction effects. Experimental data of two metallic materials are used to validate the proposed model. The agreement between the model prediction and experimental data is observed, and the predictions by proposed model are more possibly in accordance with experimental data than that by primary model and Miner's rule. Comparison between the predicted cumulative damage by the proposed model and an existing model shows that the proposed model predictions can meet the accuracy requirement of the engineering project and it can be used to predict the fatigue life of welded aluminum alloy joint of Electric Multiple Units (EMU); meanwhile, the accuracy of approximation can be obtained from the proposed model though more simple computing process and less material parameters calling for extensive testing than the existing model. PMID:24574866

  5. Isothermal Fatigue, Damage Accumulation, and Life Prediction of a Woven PMC

    NASA Technical Reports Server (NTRS)

    Gyekenyesi, Andrew L.

    1998-01-01

    This dissertation focuses on the characterization of the fully reversed fatigue behavior exhibited by a carbon fiber/polyimide resin, woven laminate at room and elevated temperatures. Nondestructive video edge view microscopy and destructive sectioning techniques were used to study the microscopic damage mechanisms that evolved. The residual elastic stiffness was monitored and recorded throughout the fatigue life of the coupon. In addition, residual compressive strength tests were conducted on fatigue coupons with various degrees of damage as quantified by stiffness reduction. Experimental results indicated that the monotonic tensile properties were only minimally influenced by temperature, while the monotonic compressive and fully reversed fatigue properties displayed noticeable reductions due to the elevated temperature. The stiffness degradation, as a function of cycles, consisted of three stages; a short-lived high degradation period, a constant degradation rate segment composing the majority of the life, and a final stage demonstrating an increasing rate of degradation up to failure. Concerning the residual compressive strength tests at room and elevated temperatures, the elevated temperature coupons appeared much more sensitive to damage. At elevated temperatures, coupons experienced a much larger loss in compressive strength when compared to room temperature coupons with equivalent damage. The fatigue damage accumulation law proposed for the model incorporates a scalar representation for damage, but admits a multiaxial, anisotropic evolutionary law. The model predicts the current damage (as quantified by residual stiffness) and remnant life of a composite that has undergone a known load at temperature. The damage/life model is dependent on the applied multiaxial stress state as well as temperature. Comparisons between the model and data showed good predictive capabilities concerning stiffness degradation and cycles to failure.

  6. Evaluation of fatigue damage accumulation in composites via linear and nonlinear guided wave methods

    NASA Astrophysics Data System (ADS)

    Zhao, Jinling; Chillara, Vamshi; Cho, Hwanjeong; Qiu, Jinhao; Lissenden, Cliff

    2016-02-01

    For non-destructive evaluation (NDE) of fatigue damage accumulation in composites, this research proposed a combined linear and a nonlinear ultrasonic guided wave method. For the linear Lamb waves approach, a laser-generation based imaging system (LGBI) is utilized to measure the phase velocities of guided waves in composites. The elastic moduli of the specimen are then obtained by inverting the measured phase velocities using genetic algorithms (GAs). The variation of the above two parameters (phase velocity and elastic moduli), together with the guided wave amplitudes, are then observed during the fatigue process. Nonlinear second harmonics in composites are studied theoretically and numerically. A third-order strain energy function of transversely isotropic materials is expressed by five invariants of the Green-Lagrange strain tensor. Results enable intelligent selection of primary modes for cumulative second harmonics generation. Meanwhile, finite element simulations are conducted to characterize second harmonics in light of the theory.

  7. Dependence of Microelastic-plastic Nonlinearity of Martensitic Stainless Steel on Fatigue Damage Accumulation

    NASA Technical Reports Server (NTRS)

    Cantrell, John H.

    2006-01-01

    Self-organized substructural arrangements of dislocations formed in wavy slip metals during cyclic stress-induced fatigue produce substantial changes in the material microelastic-plastic nonlinearity, a quantitative measure of which is the nonlinearity parameter Beta extracted from acoustic harmonic generation measurements. The contributions to Beta from the substructural evolution of dislocations and crack growth for fatigued martensitic 410Cb stainless steel are calculated from the Cantrell model as a function of percent full fatigue life to fracture. A wave interaction factor f(sub WI) is introduced into the model to account experimentally for the relative volume of material fatigue damage included in the volume of material swept out by an interrogating acoustic wave. For cyclic stress-controlled loading at 551 MPa and f(sub WI) = 0.013 the model predicts a monotonic increase in Beta from dislocation substructures of almost 100 percent from the virgin state to roughly 95 percent full life. Negligible contributions from cracks are predicted in this range of fatigue life. However, over the last five percent of fatigue life the model predicts a rapid monotonic increase of Beta by several thousand percent that is dominated by crack growth. The theoretical predictions are in good agreement with experimental measurements of 410Cb stainless steel samples fatigued in uniaxial, stress-controlled cyclic loading at 551 MPa from zero to full tensile load with a measured f(sub WI) of 0.013.

  8. Dependence of microelastic-plastic nonlinearity of martensitic stainless steel on fatigue damage accumulation

    SciTech Connect

    Cantrell, John H.

    2006-09-15

    Self-organized substructural arrangements of dislocations formed during cyclic stress-induced fatigue of metals produce substantial changes in the material microelastic-plastic nonlinearity, a quantitative measure of which is the nonlinearity parameter {beta} extracted from acoustic harmonic generation measurements. The contributions to {beta} from the substructural evolution of dislocations and crack growth for fatigued martensitic 410Cb stainless steel are calculated from the Cantrell model [Proc. R. Soc. London, Ser. A 460, 757 (2004)] as a function of percent full fatigue life to fracture. A wave interaction factor f{sub WI} is introduced into the model to account experimentally for the relative volume of fatigue damage included in the total volume of material swept out by an interrogating acoustic wave. For cyclic stress-controlled loading at 551 MPa and f{sub WI}=0.013 the model predicts a monotonic increase in {beta} from dislocation substructures of almost 100% from the virgin state to roughly 95% full life. Negligible contributions from cracks are predicted in this range of fatigue life. However, during the last 5% of fatigue life the model predicts a rapid monotonic increase of {beta} by several thousand percent that is dominated by crack growth. The theoretical predictions are in good agreement with experimental measurements of 410Cb stainless steel samples fatigued in uniaxial, stress-controlled cyclic loading at 551 MPa from zero to full tensile load with a measured f{sub WI} of 0.013.

  9. On fatigue damage accumulation from in-line and cross-flow vortex-induced vibrations on risers

    NASA Astrophysics Data System (ADS)

    Baarholm, G. S.; Larsen, C. M.; Lie, H.

    2006-01-01

    Large-scale model tests of a tensioned steel riser were performed at Hanøytangen outside Bergen, Norway in 1997. The length of the model was 90 m and the diameter was 3 cm. The information from these tests consists of measured bending strains, tension, flow speed and all relevant riser data. In this work, this information is reexamined in an attempt to improve our understanding of vortex-induced vibrations (VIV) for cases with very high order of responding modes. The aim is in particular to study the relative importance of in-line (IL) and cross-flow (CF) vibrations for fatigue damage accumulation. It is shown that fatigue damage is proportional to U (U is the flow velocity) when the modes are dominated by tension. When bending controls the modes, the fatigue damage is proportional to U. A linear SN-curve with slope parameter m=3 is used. The Hanøytangen riser fatigue damage goes as U7 for the lowest velocities and U4 for the highest current velocities. Based on the Hanøytangen data, it seems that the transition velocity between the tension and the bending-stiffness-dominated regions is at the current velocity that gives response at a mode number where a tensioned string and an untensioned beam have equal eigenfrequencies. IL response has a significant contribution to fatigue for cases dominated by the lowest modes. The reason is that IL oscillations will take place at double the frequency of those in CF. For a tension-controlled case, this corresponds to a mode with half the wavelength, while a bending-controlled case will tend to have a wavelength ratio of 2. Since the curvature for a given amplitude increases with the inverse modal wavelength squared, fatigue from IL tends to dominate for cases with tension-controlled modes (low current speed), while CF will dominate for bending-controlled modes (high current speed). This tendency is clearly seen in the experimental data for both CF and IL responses. Fatigue damage is calculated directly from the measured

  10. Quantitative Assessment of Fatigue Damage Accumulation in Wavy Slip Metals from Acoustic Harmonic Generation

    NASA Technical Reports Server (NTRS)

    Cantrell, John H.

    2006-01-01

    A comprehensive, analytical treatment is presented of the microelastic-plastic nonlinearities resulting from the interaction of a stress perturbation with dislocation substructures (veins and persistent slip bands) and cracks that evolve during high-cycle fatigue of wavy slip metals. The nonlinear interaction is quantified by a material (acoustic) nonlinearity parameter beta extracted from acoustic harmonic generation measurements. The contribution to beta from the substructures is obtained from the analysis of Cantrell [Cantrell, J. H., 2004, Proc. R. Soc. London A, 460, 757]. The contribution to beta from cracks is obtained by applying the Paris law for crack propagation to the Nazarov-Sutin crack nonlinearity equation [Nazarov, V. E., and Sutin, A. M., 1997, J. Acoust. Soc. Am. 102, 3349]. The nonlinearity parameter resulting from the two contributions is predicted to increase monotonically by hundreds of percent during fatigue from the virgin state to fracture. The increase in beta during the first 80-90 percent of fatigue life is dominated by the evolution of dislocation substructures, while the last 10-20 percent is dominated by crack growth. The model is applied to the fatigue of aluminium alloy 2024-T4 in stress-controlled loading at 276MPa for which experimental data are reported. The agreement between theory and experiment is excellent.

  11. Probabilistic Fatigue Damage Program (FATIG)

    NASA Technical Reports Server (NTRS)

    Michalopoulos, Constantine

    2012-01-01

    FATIG computes fatigue damage/fatigue life using the stress rms (root mean square) value, the total number of cycles, and S-N curve parameters. The damage is computed by the following methods: (a) traditional method using Miner s rule with stress cycles determined from a Rayleigh distribution up to 3*sigma; and (b) classical fatigue damage formula involving the Gamma function, which is derived from the integral version of Miner's rule. The integration is carried out over all stress amplitudes. This software solves the problem of probabilistic fatigue damage using the integral form of the Palmgren-Miner rule. The software computes fatigue life using an approach involving all stress amplitudes, up to N*sigma, as specified by the user. It can be used in the design of structural components subjected to random dynamic loading, or by any stress analyst with minimal training for fatigue life estimates of structural components.

  12. Compact bone fatigue damage: a microscopic examination.

    PubMed

    Carter, D R; Hayes, W C

    1977-01-01

    Flexural fatigue tests of bovine bone specimens produced fracture surfaces that were transverse on the tension side and oblique on the compression side. Similar fracture patterns were produced by bending tests with a single applied loading. Microscopic examination of flexural fatigue specimens prior to complete established that fatigue fracture is caused by the progressive accumulation of diffuse structural damage. The microdamage observed on the tension side consisted primarily of separation (or debonding) at cement lines and interlamellar cement bands. Tensile cracks in interstitial bone were also observed. The major damage modes on the compression side were oblique cracking and longitudinal splitting. The fatigue fracture patterns observed for the bone specimens correspond to the types of fatigue fractures observed clinically. Compact bone fatigue fractures in areas of longitudinal tensile stresses are generally seen as transverse lesions, whereas fatigue fractures in areas of longitudinal compressive stresses are normally oblique fractures. The diffuse nature of the observed fatigue damage is consistent with the hypothesis that microdamage caused by mechanical loading may serve as a stimulus for in vivo bone remodeling.

  13. Modeling Fatigue Damage in Long-Fiber Thermoplastics

    SciTech Connect

    Nguyen, Ba Nghiep; Kunc, Vlastimil; Bapanapalli, Satish K.

    2009-10-30

    This paper applies a fatigue damage model recently developed for injection-molded long-fiber thermoplastics (LFTs) to predict the modulus reduction and fatigue lifetime of glass/polyamide 6,6 (PA6,6) specimens. The fatigue model uses a multiscale mechanistic approach to describe fatigue damage accumulation in these materials subjected to cyclic loading. Micromechanical modeling using a modified Eshelby-Mori-Tanaka approach combined with averaging techniques for fiber length and orientation distributions is performed to establish the stiffness reduction relation for the composite as a function of the microcrack volume fraction. Next, continuum damage mechanics and a thermodynamic formulation are used to derive the constitutive relations and the damage evolution law. The fatigue damage model has been implemented in the ABAQUS finite element code and has been applied to analyze fatigue of the studied glass/PA6,6 specimens. The predictions agree well with the experimental results.

  14. Nondestructive characterization of fatigue damage with thermography

    NASA Astrophysics Data System (ADS)

    Roesner, Henrik; Sathish, Shamachary; Meyendorf, Norbert

    2001-08-01

    A thermal imaging NDE method has been developed for nondestructive characterization of early stages of fatigue damage. The method is based on evaluation of the thermal effects induced in a material by a short-term mechanical loading. The mechanical loading causes in addition to thermoelastic temperature change, an increase due to heat dissipation that depends upon the microstructure of the material in a characteristic manner. The origin of this heat dissipation is the mechanical damping process. Utilizing the initial temperature rise due to a short-term mechanical loading, the dissipated energy per cycle was evaluated as a thermal parameter. This new thermal NDE parameter allows a quantitative characterization of the mechanical hysteresis, without the need for calibration to eliminate influences of thermal boundary conditions. The measurement of the thermal NDE parameters has been performed on Ti-6Al-4V dog-bone specimens, fatigued in low cycle fatigue (LCF) as well as in high cycle fatigue (HCF) experiments. Characteristic dependence of the NDE parameters on the already accumulated fatigue damage has been observed. The advantage of the thermal method is the applicability to components under service conditions because of simplicity, rapid measurements (a few seconds) and the ability of locally resolved evaluations.

  15. Cumulative creep fatigue damage in 316 stainless steel

    NASA Technical Reports Server (NTRS)

    Mcgaw, Michael A.

    1989-01-01

    The cumulative creep-fatigue damage behavior of 316 stainless steel at 1500 F was experimentally established for the two-level loading cases of fatigue followed by fatigue, creep fatigue followed by fatigue, and fatigue followed by creep fatigue. The two-level loadings were conducted such that the lower life (high strain) cycling was applied first for a controlled number of cycles and the higher life (low strain) cycling was conducted as the second level to failure. The target life levels in this study were 100 cycles to failure for both the fatigue and creep-fatigue lowlife loading, 5000 cycles to failure for the higher life fatigue loading and 10,000 cycles to failure for the higher life creep-fatigue loading. The failed specimens are being examined both fractographically and metallographically to ascertain the nature of the damaging mechanisms that produced failure. Models of creep-fatigue damage accumulation are being evaluated and knowledge of the various damaging mechanisms is necessary to ensure that predictive capability is instilled in the final failure model.

  16. Development of the electrochemical fatigue sensor for evaluating fatigue damage

    SciTech Connect

    Li, Y.F.; Wang, J.; Wang, M.Z.; DeLuccia, J.; Laird, C.

    1999-07-01

    The Electrochemical Fatigue Sensor (EFS) is a device which operates by an electrochemical-mechanical interaction and which can sense the type and extent of fatigue damage both before and after crack initiation. It was initially explored through studies on soft metals. Here the authors report efforts to determine the ability of the device to read damage in hardened commercial alloys: 7075 aluminum alloy, 4130 steel and Ti-6Al-4V. They also demonstrate that the device, which uses an electrolytic medium, does not degrade the fatigue properties if care is used in electrolyte selection.

  17. An Approach for Nonlinear Fatigue Damage Evaluation in Asphalt Pavements

    NASA Astrophysics Data System (ADS)

    Rajbongshi, Pabitra; Thongram, Sonika

    2016-08-01

    Fatigue due to vehicular loads is one of the primary distress mechanisms in asphalt pavements. It happens primarily due to deterioration in asphalt material with load repetitions. Degradation of asphalt material may be evaluated using different parameters. In view of degradation, the incremental damage in a given pavement section would be different for different repetitions, even with same loadings. Therefore, the damage progression becomes nonlinear with repetitions. Accounting such nonlinearity in damage accumulation, and based on different damage evaluation parameters, this paper presents an equivalent approach for fatigue damage evaluation in asphalt pavements. Traditional fatigue equation adopted in mechanistic-empirical pavement design has been used in the present work. Four different criteria, namely number of load repetitions, asphalt stiffness reduction, strain enhancement and fatigue life reduction with repetitions are considered for damage estimation. The proposed approach could estimate same value of nonlinear damage, irrespective of the criteria used. The simplest form of criterion i.e. the number of load repetitions can be used for fatigue performance evaluation. Probabilistically, the damage propagation is also correlated and assessed with the failure probability.

  18. Finite element prediction of fatigue damage growth in cancellous bone.

    PubMed

    Hambli, Ridha; Frikha, Sana; Toumi, Hechmi; Tavares, João Manuel R S

    2016-01-01

    Cyclic stresses applied to bones generate fatigue damage that affects the bone stiffness and its elastic modulus. This paper proposes a finite element model for the prediction of fatigue damage accumulation and failure in cancellous bone at continuum scale. The model is based on continuum damage mechanics and incorporates crack closure effects in compression. The propagation of the cracks is completely simulated throughout the damaged area. In this case, the stiffness of the broken element is reduced by 98% to ensure no stress-carrying capacities of completely damaged elements. Once a crack is initiated, the propagation direction is simulated by the propagation of the broken elements of the mesh. The proposed model suggests that damage evolves over a real physical time variable (cycles). In order to reduce the computation time, the integration of the damage growth rate is based on the cycle blocks approach. In this approach, the real number of cycles is reduced (divided) into equivalent blocks of cycles. Damage accumulation is computed over the cycle blocks and then extrapolated over the corresponding real cycles. The results show a clear difference between local tensile and compressive stresses on damage accumulation. Incorporating stiffness reduction also produces a redistribution of the peak stresses in the damaged region, which results in a delay in damage fracture.

  19. The relationship between observed fatigue damage and life estimation models

    NASA Technical Reports Server (NTRS)

    Kurath, Peter; Socie, Darrell F.

    1988-01-01

    Observations of the surface of laboratory specimens subjected to axial and torsional fatigue loadings has resulted in the identification of three damage fatigue phenomena: crack nucleation, shear crack growth, and tensile crack growth. Material, microstructure, state of stress/strain, and loading amplitude all influence which of the three types of fatigue damage occurs during a dominant fatigue life fraction. Fatigue damage maps are employed to summarize the experimental observations. Appropriate bulk stress/strain damage parameters are suggested to model fatigue damage for the dominant fatigue life fraction. Extension of the damage map concept to more complex loadings is presented.

  20. Damage mechanics characterization on fatigue behavior of a solder joint material

    SciTech Connect

    Chow, C.L.; Yang, F.; Fang, H.E.

    1998-08-01

    This paper presents the first part of a comprehensive mechanics approach capable of predicting the integrity and reliability of solder joint material under fatigue loading without viscoplastic damage considerations. A separate report will be made to present a comprehensive damage model describing life prediction of the solder material under thermomechanical fatigue loading. The method is based on a theory of damage mechanics which makes possible a macroscopic description of the successive material deterioration caused by the presence of microcracks/voids in engineering materials. A damage mechanics model based on the thermodynamic theory of irreversible processes with internal state variables is proposed and used to provide a unified approach in characterizing the cyclic behavior of a typical solder material. With the introduction of a damage effect tensor, the constitutive equations are derived to enable the formulation of a fatigue damage dissipative potential function and a fatigue damage criterion. The fatigue evolution is subsequently developed based on the hypothesis that the overall damage is induced by the accumulation of fatigue and plastic damage. This damage mechanics approach offers a systematic and versatile means that is effective in modeling the entire process of material failure ranging from damage initiation and propagation leading eventually to macro-crack initiation and growth. As the model takes into account the load history effect and the interaction between plasticity damage and fatigue damage, with the aid of a modified general purpose finite element program, the method can readily be applied to estimate the fatigue life of solder joints under different loading conditions.

  1. Periostin deficiency increases bone damage and impairs injury response to fatigue loading in adult mice.

    PubMed

    Bonnet, Nicolas; Gineyts, Evelyne; Ammann, Patrick; Conway, Simon J; Garnero, Patrick; Ferrari, Serge

    2013-01-01

    Bone damage removal and callus formation in response to fatigue loading are essential to prevent fractures. Periostin (Postn) is a matricellular protein that mediates adaptive response of cortical bone to loading. Whether and how periostin influences damage and the injury response to fatigue remains unknown. We investigated the skeletal response of Postn(-/-) and Postn(+/+) mice after fatigue stimulus by axial compression of their tibia. In Postn(+/+) mice, cracks number and surface (CsNb, CsS) increased 1h after fatigue, with a decrease in strength compared to non-fatigued tibia. At 15 days, CsNb had started to decline, while CtTV and CtBV increased in fatigued vs non-fatigued tibia, reflecting a woven bone response that was present in 75% of the fatigued bones. Cortical porosity and remodelling also prominently increased in the fatigued tibia of Postn(+/+) mice. At 30 days, paralleling a continuous removal of cortical damage, strength of the fatigued tibia was similar to the non-fatigue tibia. In Postn(-/-) mice, cracks were detectable even in the absence of fatigue, while the amount of collagen crosslinks and tissue hardness was decreased compared to Postn(+/+). Fatigue significantly increased CsNb and CsS in Postn(-/-), but was not associated with changes in CtTV and CtBV, as only 16% of the fatigued bones formed some woven bone. Cortical porosity and remodelling did not increase either after fatigue in Postn(-/-), and the level of damage remained high even after 30 days. As a result, strength remained compromised in Postn(-/-) mice. Contrary to Postn(+/+), which osteocytic lacunae showed a change in the degree of anisotropy (DA) after fatigue, Postn(-/-) showed no DA change. Hence periostin appears to influence bone materials properties, damage accumulation and repair, including local modeling/remodeling processes in response to fatigue. These observations suggest that the level of periostin expression could influence the propensity to fatigue fractures. PMID

  2. Brittleness Effect on Rock Fatigue Damage Evolution

    NASA Astrophysics Data System (ADS)

    Nejati, Hamid Reza; Ghazvinian, Abdolhadi

    2014-09-01

    The damage evolution mechanism of rocks is one of the most important aspects in studying of rock fatigue behavior. Fatigue damage evolution of three rock types (onyx marble, sandstone and soft limestone) with different brittleness were considered in the present study. Intensive experimental tests were conducted on the chosen rock samples and acoustic emission (AE) sensors were used in some of them to monitor the fracturing process. Experimental tests indicated that brittleness strongly influences damage evolution of rocks in the course of static and dynamic loading. AE monitoring revealed that micro-crack density induced by the applied loads during different stages of the failure processes increases as rock brittleness increases. Also, results of fatigue tests on the three rock types indicated that the rock with the most induced micro-cracks during loading cycles has the least fatigue life. Furthermore, the condition of failure surfaces of the studied rocks samples, subjected to dynamic and static loading, were evaluated and it was concluded that the roughness of failure surfaces is influenced by loading types and rock brittleness. Dynamic failure surfaces were rougher than static ones and low brittle rock demonstrate a smoother failure surface compared to high brittle rock.

  3. Time-dependent damage in predictions of fatigue behaviour of normal and healing ligaments

    NASA Astrophysics Data System (ADS)

    Thornton, Gail M.; Bailey, Soraya J.; Schwab, Timothy D.

    2015-08-01

    Ligaments are dense fibrous tissues that connect bones across a joint and are exposed daily to creep and fatigue loading. Ligaments are tensile load-bearing tissues; therefore, fatigue loading will have a component of time-dependent damage from the non-zero mean stress and cycle-dependent damage from the oscillating stress. If time-dependent damage is not sufficient to completely predict the fatigue response, then cycle-dependent damage could be an important contributor. Using data from normal ligaments (current study and Thornton et al., Clin. Biomech. 22:932-940, 2007a) and healing ligaments (Thornton and Bailey, J. Biomech. Eng. 135:091004-1-091004-6, 2013), creep data was used to predict the fatigue response considering time-dependent damage. Relationships between creep lifetime and test stress or initial strain were modelled using exponential or power-law regression. In order to predict fatigue lifetimes, constant rates of damage were assumed and time-varying stresses were introduced into the expressions for time-dependent damage from creep. Then, the predictions of fatigue lifetime were compared with curvefits to the fatigue data where exponential or power-law regressions were used to determine the relationship between fatigue lifetime and test stress or initial strain. The fatigue prediction based on time-dependent damage alone greatly overestimated fatigue lifetime suggesting that time-dependent damage alone cannot account for all of the damage accumulated during fatigue and that cycle-dependent damage has an important role. At lower stress and strain, time-dependent damage was a greater relative contributor for normal ligaments than healing ligaments; however, cycle-dependent damage was a greater relative contributor with incremental increases in stress or strain for normal ligaments than healing ligaments.

  4. Fatigue damage-fracture mechanics interaction in cortical bone.

    PubMed

    Yeni, Y N; Fyhrie, D P

    2002-03-01

    Fatigue loading causes accumulation of damage that may lead to the initiation of a macrocrack and result in a catastrophic failure of bone. The objective of this study was to examine the influence of fatigue damage on crack growth parameters in bovine cortical bone. Nineteen rectangular beam specimens (4 x 4 x 48 mm) were machined from bovine tibiae. The long axis of the beams was aligned with the long axis of bones. Using a four-point bending fatigue setup, ten specimens were fatigue-damaged to different levels as indicated by stiffness loss. A through-thickness notch was machined at the center of each damaged and undamaged beam. The notched specimens were then monotonically loaded beyond failure using a three-point bending protocol. Critical stress intensity factor, K(I), and work to critical load, W(Q), were significantly lower in the damaged group than in the undamaged group (p < 0.03). When the undamaged specimens were assigned a percent stiffness loss of zero and pooled with the damaged group, significant negative correlations of percent stiffness loss with K(I) (R = 0.58, p < 0.01), W(Q) (R = 0.54, p < 0.02), maximum load, P(max) (R = 0.59, p < 0.008), deflection at maximum load, Delta(max) (R = 0.48, p < 0.04), structural stiffness, S(max) (R = 0.53, p < 0.02), W(max) (R = 0.55, p < 0.02), and load at 1.4 mm deflection (a value beyond failure but without complete fracture), P(1.4) (R = 0.47, p < 0.05), were found. Post hoc analysis revealed that the average load-deflection curve from the damaged group was transformable into that from the undamaged group through a special shift on the load-deflection plane. Fatigue damage reduces bone stiffness and resistance to crack initiation, maximum load-carrying capacity, and deflection before and after failure in cortical bone. The data suggest there is a single rule that governs the overall effect of fatigue damage on the fracture behavior of cortical bone. PMID:11882466

  5. Fatigue damage analysis under variable amplitude cycling

    NASA Technical Reports Server (NTRS)

    Leis, B. N.; Forte, T. P.

    1983-01-01

    This paper explores the suitability of a recently proposed mean stress parameter and introduces a nonlinear damage accumulation procedure. Data covering a range of positive and negative stress ratios from +0.6 to -2.66, for several aluminum alloys and steels, are assembled and shown to be well correlated by a simple damage parameter. A nonlinear damage accumulation postulate is advanced to replace the usual linear procedure. Results of critical experiments performed to assess the suitability of the postulate are introduced and shown to support a non-linear criterion. The implications of this work related to variable amplitude life prediction are discussed.

  6. Accumulated damage process of thermal sprayed coating under rolling contact by acoustic emission technique

    NASA Astrophysics Data System (ADS)

    Xu, Jia; Zhou, Zhen-yu; Piao, Zhong-yu

    2016-09-01

    The accumulated damage process of rolling contact fatigue (RCF) of plasma-sprayed coatings was investigated. The influences of surface roughness, loading condition, and stress cycle frequency on the accumulated damage status of the coatings were discussed. A ball-ondisc machine was employed to conduct RCF experiments. Acoustic emission (AE) technique was introduced to monitor the RCF process of the coatings. AE signal characteristics were investigated to reveal the accumulated damage process. Result showed that the polished coating would resist the asperity contact and remit accumulated damage. The RCF lifetime would then extend. Heavy load would aggravate the accumulated damage status and induce surface fracture. Wear became the main failure mode that reduced the RCF lifetime. Frequent stress cycle would aggravate the accumulated damage status and induce interface fracture. Fatigue then became the main failure mode that also reduced the RCF lifetime.

  7. Accumulated damage process of thermal sprayed coating under rolling contact by acoustic emission technique

    NASA Astrophysics Data System (ADS)

    Xu, Jia; Zhou, Zhen-yu; Piao, Zhong-yu

    2016-07-01

    The accumulated damage process of rolling contact fatigue (RCF) of plasma-sprayed coatings was investigated. The influences of surface roughness, loading condition, and stress cycle frequency on the accumulated damage status of the coatings were discussed. A ball-ondisc machine was employed to conduct RCF experiments. Acoustic emission (AE) technique was introduced to monitor the RCF process of the coatings. AE signal characteristics were investigated to reveal the accumulated damage process. Result showed that the polished coating would resist the asperity contact and remit accumulated damage. The RCF lifetime would then extend. Heavy load would aggravate the accumulated damage status and induce surface fracture. Wear became the main failure mode that reduced the RCF lifetime. Frequent stress cycle would aggravate the accumulated damage status and induce interface fracture. Fatigue then became the main failure mode that also reduced the RCF lifetime.

  8. An experimental study of damage accumulation in cemented hip prostheses.

    PubMed

    McCormack, B A O; Prendergast, P J; Gallagher, D G

    1996-06-01

    OBJECTIVE: To develop a methodology to characterize the pattern of crack initiation and damage accumulation in intramedullary fixated cemented prostheses. DESIGN: An experimental physical model of intramedullary fixation was developed which both represents the implant structure and permits monitoring of fatigue crack growth. BACKGROUND: Many joint replacement prostheses are fixed into the medullary cavity of bones using a poly(methylmethacrylate) 'bone cement', which forms a mantle around the prosthesis and locks it to the bone. The endurance of the replacement is, to a great extent, determined by the mechanical durability of the cement and the implant interfaces under cyclic stresses generated by dynamic loading. The cement mantle is subjected to complex multiaxial stresses which vary in particular distribution depending on the prosthesis design. METHODS: Damage accumulation is reported in terms of the number of cracks, the location of cracks, and the rate of crack growth. RESULTS: The results clearly show the nature of damage accumulation in the cement mantle, and that many of the cracks which propagate within the cement mantle are related to cement porosity. CONCLUSION: This study gives experimental evidence to support the hypothesis of a damage accumulation failure scenario in cemented hip reconstructions. RELEVANCE: Cementing is the most popular technique for the fixation of joint replacement prosthesis. However, the sequence of events leading to the failure of cemented fixation is not fully understood. In this paper it is shown that damage accumulation can be directly monitored in an experimental model of cemented intramedullary fixation.

  9. Life prediction modeling based on cyclic damage accumulation

    NASA Technical Reports Server (NTRS)

    Nelson, Richard S.

    1988-01-01

    A high temperature, low cycle fatigue life prediction method was developed. This method, Cyclic Damage Accumulation (CDA), was developed for use in predicting the crack initiation lifetime of gas turbine engine materials, where initiation was defined as a 0.030 inch surface length crack. A principal engineering feature of the CDA method is the minimum data base required for implementation. Model constants can be evaluated through a few simple specimen tests such as monotonic loading and rapic cycle fatigue. The method was expanded to account for the effects on creep-fatigue life of complex loadings such as thermomechanical fatigue, hold periods, waveshapes, mean stresses, multiaxiality, cumulative damage, coatings, and environmental attack. A significant data base was generated on the behavior of the cast nickel-base superalloy B1900+Hf, including hundreds of specimen tests under such loading conditions. This information is being used to refine and extend the CDA life prediction model, which is now nearing completion. The model is also being verified using additional specimen tests on wrought INCO 718, and the final version of the model is expected to be adaptable to most any high-temperature alloy. The model is currently available in the form of equations and related constants. A proposed contract addition will make the model available in the near future in the form of a computer code to potential users.

  10. Comprehensive model of damage accumulation in silicon

    SciTech Connect

    Mok, K. R. C.; Benistant, F.; Jaraiz, M.; Rubio, J. E.; Castrillo, P.; Pinacho, R.; Srinivasan, M. P.

    2008-01-01

    Ion implantation induced damage accumulation is crucial to the simulation of silicon processing. We present a physically based damage accumulation model, implemented in a nonlattice atomistic kinetic Monte Carlo simulator, that can simulate a diverse range of interesting experimental observations. The model is able to reproduce the ion-mass dependent silicon amorphous-crystalline transition temperature of a range of ions from C to Xe, the amorphous layer thickness for a range of amorphizing implants, the superlinear increase in damage accumulation with dose, and the two-layered damage distribution observed along the path of a high-energy ion. In addition, this model is able to distinguish between dynamic annealing and post-cryogenic implantation annealing, whereby dynamic annealing is more effective in removing damage than post-cryogenic implantation annealing at the same temperature.

  11. Fatigue testing and damage development in continuous fiber reinforced metal matrix composites

    NASA Technical Reports Server (NTRS)

    Johnson, W. S.

    1988-01-01

    A general overview of the fatigue behavior of metal matrix composites (MMC) is presented. The first objective is to present experimental procedures and techniques for conducting a meaningful fatigue test to detect and quantify fatigue damage in MMC. These techniques include interpretation of stress-strain responses, acid etching of the matrix, edge replicas of the specimen under load, radiography, and micrographs of the failure surfaces. In addition, the paper will show how stiffness loss in continuous fiber reinforced metal matrix composites can be a useful parameter for detecting fatigue damage initiation and accumulation. Second, numerous examples of how fatigue damage can initiate and grow in various MMC are given. Depending on the relative fatigue behavior of the fiber and matrix, and the interface properties, the failure modes of MMC can be grouped into four categories: (1) matrix dominated, (2) fiber dominated, (3) self-similar damage growth, and (4) fiber/matrix interfacial failures. These four types of damage will be discussed and illustrated by examples with the emphasis on the fatigue of unnotched laminates.

  12. Fatigue testing and damage development in continuous fiber reinforced metal matrix composites

    NASA Technical Reports Server (NTRS)

    Johnson, W. S.

    1989-01-01

    A general overview of the fatigue behavior of metal matrix composites (MMC) is presented. The first objective is to present experimental procedures and techniques for conducting a meaningful fatigue test to detect and quantify fatigue damage in MMC. These techniques include interpretation of stress-strain responses, acid etching of the matrix, edge replicas of the specimen under load, radiography, and micrographs of the failure surfaces. In addition, the paper will show how stiffness loss in continuous fiber reinforced metal matrix composites can be a useful parameter for detecting fatigue damage initiation and accumulation. Second, numerous examples of how fatigue damage can initiate and grow in various MMC are given. Depending on the relative fatigue behavior of the fiber and matrix, and the interface properties, the failure modes of MMC can be grouped into four categories: (1) matrix dominated, (2) fiber dominated, (3) self-similar damage growth, and (4) fiber/matrix interfacial failures. These four types of damage will be discussed and illustrated by examples with the emphasis on the fatigue of unnotched laminates.

  13. Fatigue Damage in CFRP Woven Fabric Composites through Dynamic Modulus Measurements

    SciTech Connect

    Chiaki Miyasaka; K. L. Telschow

    2004-07-01

    Advanced fiber reinforced composite materials offer substantial advantages over metallic materials for the structural applications subjected to fatigue loading. With the increasing use of these composites, it is required to understand their mechanical response to cyclic loading (1)-(4). Our major concern in this work is to macroscopically evaluate the damage development in composites during fatigue loading. For this purpose, we examine what effect the fatigue damage may have on the material properties and how they can be related mathematically to each other. In general, as the damage initiates in composite materials and grows during cyclic loading, material properties such as modulus, residual strength and strain would vary and, in many cases, they may be significantly reduced because of the progressive accumulation of cracks. Therefore, the damage can be characterized by the change in material properties, which is expected to be available for non-destructive evaluation of the fatigue damage development in composites. Here, the tension-tension fatigue tests are firstly conducted on the plain woven fabric carbon fiber composites for different loading levels. In the fatigue tests, the dynamic elastic moduli are measured on real-time, which will decrease with an increasing number of cycles due to the degradation of stiffness. Then, the damage function presenting the damage development during fatigue loading is determined from the dynamic elastic moduli thus obtained, from which the damage function is formulated in terms of a number of cycles and an applied loading level. Finally, the damage function is shown to be applied for predicting the remaining lifetime of the CFRP composites subjected to two-stress level fatigue loading.

  14. Fatigue-Induced Damage in Zr-Based Bulk Metallic Glasses

    PubMed Central

    Chuang, Chih-Pin; Yuan, Tao; Dmowski, Wojciech; Wang, Gong-Yao; Freels, Matt; Liaw, Peter K.; Li, Ran; Zhang, Tao

    2013-01-01

    In the present work, we investigate the effect of “fatigue” on the fatigue behavior and atomic structure of Zr-based BMGs. Fatigue experiments on the failed-by-fatigue samples indicate that the remnants generally have similar or longer fatigue life than the as-cast samples. Meanwhile, the pair-distribution-function (PDF) analysis of the as-cast and post-fatigue samples showed very small changes of local atomic structures. These observations suggest that the fatigue life of the 6-mm in-diameter Zr-based BMG is dominated by the number of pre-existing crack-initiation sites in the sample. Once the crack initiates in the specimen, the fatigue-induced damage is accumulated locally on these initiated sites, while the rest of the region deforms elastically. The results suggest that the fatigue failure of BMGs under compression-compression fatigue experiments is a defect-controlled process. The present work indicates the significance of the improved fatigue resistance with decreasing the sample size. PMID:23999496

  15. Fatigue Life of Postbuckled Structures with Indentation Damages

    NASA Technical Reports Server (NTRS)

    Davila, Carlos G.; Bisagni, Chiara

    2016-01-01

    The fatigue life of composite stiffened panels with indentation damage was investigated experimentally using single stringer compression specimens. Indentation damage was induced on one of the two flanges of each stringer. The experiments were conducted using advanced instrumentation, including digital image correlation, passive thermography, and in-situ ultrasonic scanning. Specimens with initial indentation damage lengths of 32 millimeters to 56 millimeters were tested quasi-statically and in fatigue, and the effects of cyclic load amplitude and damage size were studied. A means of comparison of the damage propagation rates and collapse loads based on a stress intensity measure and the Paris law is proposed.

  16. Fatigue Life of Postbuckled Structures with Indentation Damage

    NASA Technical Reports Server (NTRS)

    Davila, Carlos G.; Bisagni, Chiara

    2016-01-01

    The fatigue life of composite stiffened panels with indentation damage was investigated experimentally using single stringer compression specimens. Indentation damage was induced on one of the two flanges of the stringer. The experiments were conducted using advanced instrumentation, including digital image correlation, passive thermography, and in-situ ultrasonic scanning. Specimens with initial indentation damage lengths of 37 millimeters to 56 millimeters were tested in fatigue and the effects of cyclic load amplitude and damage size were studied. A means of comparison of the damage propagation rates and collapse loads based on a stress intensity measure and the Paris law is proposed.

  17. Topology optimization in damage governed low cycle fatigue

    NASA Astrophysics Data System (ADS)

    Desmorat, Boris; Desmorat, Rodrigue

    2008-05-01

    Topology optimization is applied here to discuss an optimization problem of fatigue resistance. Fatigue lifetime is maximized by optimizing the shape of a structure in cyclic plasticity combined with Lemaitre damage law. The topology optimization algorithm is detailed. A 3D numerical example is given. To cite this article: B. Desmorat, R. Desmorat, C. R. Mecanique 336 (2008).

  18. Mean stress and the exhaustion of fatigue-damage resistance

    NASA Technical Reports Server (NTRS)

    Berkovits, Avraham

    1989-01-01

    Mean-stress effects on fatigue life are critical in isothermal and thermomechanically loaded materials and composites. Unfortunately, existing mean-stress life-prediction methods do not incorporate physical fatigue damage mechanisms. An objective is to examine the relation between mean-stress induced damage (as measured by acoustic emission) and existing life-prediction methods. Acoustic emission instrumentation has indicated that, as with static yielding, fatigue damage results from dislocation buildup and motion until dislocation saturation is reached, after which void formation and coalescence predominate. Correlation of damage processes with similar mechanisms under monotonic loading led to a reinterpretation of Goodman diagrams for 40 alloys and a modification of Morrow's formulation for life prediction under mean stresses. Further testing, using acoustic emission to monitor dislocation dynamics, can generate data for developing a more general model for fatigue under mean stress.

  19. Sources of fatigue damage to passive yaw wind turbine blades

    SciTech Connect

    Laino, D.J.

    1997-12-31

    Using an integrated computer analysis approach developed at the University of Utah, fatigue damage sources to passive yaw wind turbine blades have been investigated. Models of a rigid hub and teetering hub machine reveal the parameters important to the fatigue design of each type. The teetering hub proved much less susceptible to fatigue damage from normal operation loads. As a result, extreme events were critical to the teetering hub fatigue life. The rigid hub blades experienced extremely large gyroscopic load cycles induced by rapid yaw rates during normal operation. These yaw rates stem from turbulence activity which is shown to be dependent upon atmospheric stability. Investigation revealed that increasing yaw damping is an effective way of significantly reducing these gyroscopic fatigue loads.

  20. Fractographic analysis of fatigue damage in 7000 aluminium alloys.

    PubMed

    Cvijović, Z; Vratnica, M; Gerić, K

    2008-12-01

    In this paper, an attempt is made to correlate the fatigue damage in 7000 aluminium alloys with different impurity contents to the microstructural features and to explain their interdependence through fractographic observations. The Paris constants of these alloys in the form of hot-forged plates subjected to the overaged T73 temper are evaluated and differences in the fatigue crack growth rate described by striation spacing measurements. Scanning electron microscopy analysis of fatigue fracture surfaces revealed that the type and morphological parameters of coarse intermetallic particles play a critical role in fatigue crack growth behaviour. The elemental distribution determined by means of energy-dispersive spectroscopy analysis showed that the fractured particles accelerating the crack advances are larger particles of Fe-rich phases. The fatigue crack growth rate increases considerably with increasing amounts of these particles. The smaller eta, S and Mg(2)Si particles contribute beneficially to fatigue life.

  1. Nondestructive determination of fatigue crack damage in composites using vibration tests.

    NASA Technical Reports Server (NTRS)

    Dibenedetto, A. T.; Gauchel, J. V.; Thomas, R. L.; Barlow, J. W.

    1972-01-01

    The vibration response of glass reinforced epoxy and polyester laminates was investigated. The complex modulus and the damping capacity were measured as fatigue crack damage accumulated. Changes in the Young's modulus as well as the damping capacity correlated with the amount of crack damage. The damping was especially sensitive to debonding of the reinforcement from the resin matrix. Measurement of these vibration response changes shows promise as a means to nondestructively test the structural integrity of filament-reinforced composite structural members.

  2. Fatigue damage mechanisms in boron-aluminium composite laminates

    NASA Technical Reports Server (NTRS)

    Dvorak, G. J.; Johnson, W. S.

    1980-01-01

    The relationship between fatigue and shakedown in metal matrix composites is investigated theoretically and experimentally for unidirectional and laminated 6061 Al-B materials. It is shown that no fatigue damage takes place if the applied stress range is such that the material remains elastic, or shakes down, i.e., resumes elastic cyclic straining after a small number of plastic strain cycles. Fatigue damage occurs only in specimens subjected to stress ranges which cause sustained cyclic plastic straining in the aluminum matrix. If the applied stress range is smaller than that required for fatigue failure, after about 10 to the 6th cycles a saturation damage state is reached which remains essentially unchanged with increasing number of cycles.

  3. An empirical modified fatigue damage model for impacted GFRP laminates

    NASA Astrophysics Data System (ADS)

    Naderi, S.; Hassan, M. A.; Bushroa, A. R.

    2014-10-01

    The aim of the present paper is to evaluate the residual strength of GFRP laminates following a low-velocity impact event under cyclic loading. The residual strength is calculated using a linear fatigue damage model. According to an investigation into the effect of low-velocity impact on the fatigue behavior of laminates, it seems laminate fatigue life decreases after impact. By normalizing the fatigue stress against undamaged static strength, the Fatigue Damage parameter “FD” is presented with a linear relationship as its slope which is a linear function of the initial impact energy; meanwhile, the constants were attained from experimental data. FD is implemented into a plane-stress continuum damage mechanics based model for GFRP composite laminates, in order to predict damage threshold in composite structures. An S-N curve is implemented to indicate the fatigue behavior for 2 mm thickness encompassing both undamaged and impacted samples. A decline in lifespan is evident when the impact energy level increases. Finally, the FD is intended to capture the unique GFRP composite characteristics.

  4. Detection of localized fatigue damage in steel by thermography

    NASA Astrophysics Data System (ADS)

    Medgenberg, Justus; Ummenhofer, Thomas

    2007-04-01

    Fatigue damage of unalloyed steels in the high cycle regime is governed by localized cyclic plastic deformations and subsequent crack initiation. The extent of early microplastic deformations depends on the applied stress level, stress concentration at macroscopic notches, surface treatment, residual stresses etc. The onset of a nonlinear material response can be regarded as an early indicator of fatigue damage. During fatigue loading thermoelastic coupling and thermoplastic dissipation cause characteristic temperature variations in tested specimens which have been assessed by a highly sensitive infrared camera. A specialized data processing method in the time domain has been developed which allows to separate the different contributions to the measured temperature signal. In contrast to other methods - as e.g. measuring the rise of mean temperature during fatigue loading - the proposed methodology is based on measurements during the stabilized temperature regimen and offers very high spatial resolution of localized phenomena. Investigations have been made on mildly notched cylindrical and also on welded specimens. The results confirm the close relation between the local temperature signal and typical fatigue phenomena. The new methodology allows for a much better localization and quantification of effects as cyclic plasticity, crack initiation, crack growth etc. The following paper presents considerations and experimental results of an application of thermography to the local assessment of fatigue damage.

  5. Research on fatigue damage detection for wind turbine blade based on high-spatial-resolution DPP-BOTDA

    NASA Astrophysics Data System (ADS)

    Xu, Jinlong; Dong, Yongkang; Li, Hui

    2014-03-01

    In this paper, a fatigue damage detection system used for wind turbine blade is successfully developed by using highspatial- resolution differential pulse-width pair Brillouin optical time-domain analysis (DPP-BOTDA) sensing system. A piece of polarization-maintaining optical fiber is bonded on the blade surface to form the distributed sensing network. A DPP-BOTDA system, with a spatial resolution of 20cm and sampling interval of 1cm, is adopted to measuring distributed strain and detecting fatigue damage of wind turbine blade during fatigue test using the differential pulse pair of 39.5ns/41.5ns. Strain and the Brillouin gain spectra changes from undamaged state to fatigue failure are experimentally presented. The experimental results reveal that fatigue damage changes the strain distribution especially around the high strain area, and the width, amplitude and central frequency of the Brillouin gain spectra are sensitive to fatigue damage as the stiffness degradation and accumulated cracks change local strain gradient. As the damage becomes larger, the width of the Brillouin gain spectra becomes broader. Consequently, location and size of fatigue damage could be estimated. The developed system shows its potentiality for developing highly reliable wind turbine monitoring system as the effectiveness of damage detection and distributed sensing.

  6. Reduction in fiber damage thresholds due to static fatigue

    SciTech Connect

    Setchell, R.E.

    1995-01-01

    Damage mechanisms may occur during the transmission of Q-switched, Nd/YAG laser pulses through fused silica fibers. Fiber end-face characteristics, laser characteristics, and aspects of the laser-to-fiber injection typically determine dominant damage mechanisms. However, an additional damage process has been observed at internal sites where fibers were experiencing significant local stresses due to fixturing or bends in the fiber path. A transmission reduction prior to damage was typically not measurable at these sites. Damage would not always occur during initial testing, but sometimes occurred later in time at laser levels that previously had been transmitted without damage. In these cases the time at stress appeared to be more important than the number of transmitted shots prior to damage. A possible relation between internal damage thresholds at stressed sites and the total time under stress is suggested by the fact that silica fibers experience static fatigue processes. These processes involve the slow growth of local defects under tensile stress at rates that depend upon environmental conditions. Defects reaching sufficient size and having appropriate location could be sites for reduced laser-induced damage thresholds. The present work looks into the possibility that static fatigue processes can affect damage thresholds. The experiments used a laser injection and fiber routing configuration that produced significantly elevated fluences within fiber core regions under tensile stress. In order to establish initial strength and fatigue properties for these fibers, a number of samples were used to generate time-to-failure data at various stress levels. Other fiber samples were subjected to conditions that greatly accelerated fatigue processes. Internal damage thresholds were then measured in these fibers and compared to thresholds measured in fresh fibers. Conclusive comparisons were frustrated by sample-to-sample and lot-to-lot variations in fiber defects.

  7. Isothermal fatigue behavior and damage modeling of a high temperature woven PMC[Polymer Matrix Composite

    SciTech Connect

    Gyekenyesi, A.L.

    2000-01-01

    This study focuses on the fully reversed fatigue behavior exhibited by a carbon fiber/polyimide resin woven laminate at room and elevated temperatures. Nondestructive video edge view microscopy and destructive sectioning techniques were used to study the microscopic damage mechanisms that evolved. The elastic stiffness was monitored and recorded throughout the fatigue life of the coupon. In addition, residual compressive strength tests were conducted on fatigue coupons with various degrees of damage as quantified by stiffness reduction. Experimental results indicated that the monotonic tensile properties were only minimally influenced by temperature, while the monotonic compressive and fully reversed fatigue properties displayed greater reductions due to the elevated temperature. The stiffness degradation as a function of cycles, consisted of three stages; a short-lived high degradation period, a constant degradation rate segment covering the majority of the life, and a final stage demonstrating an increasing rate of degradation up to failure. Concerning the residual compressive strength tests at room and elevated temperatures, the elevated temperature coupons appeared much more sensitive to damage. At elevated temperatures, coupons experienced a much larger loss in compressive strength when compared to room temperature coupons with equivalent damage. The fatigue damage accumulation law proposed for the model incorporates a scalar representation for damage, but admits a multiaxial, anisotropic evolutionary law. The model predicts the current damage (as quantified by residual stiffness) and remnant life of a composite that has undergone a known load at temperature. The damage/life model is dependent on the applied multiaxial stress state as well as temperature. Comparisons between the model and data showed good predictive capabilities concerning stiffness degradation and cycles to failure.

  8. A novel damage index for fatigue damage detection in a laminated composites using Lamb waves

    NASA Astrophysics Data System (ADS)

    Seki, Daigo

    A well-established structural health monitoring (SHM) technique, the Lamb wave based approach, is used for fatigue damage identification in a laminated composite. A novel damage index, 'normalized correlation moment' (NCM) which is composed of the nth moment of the cross correlation of the baseline and comparison waves, was used as damage index for monitoring damage in composites and compared with the signal difference coefficient (SDC) which is one of the most commonly used damage indices. Composite specimens were fabricated by the hand layup method by followed by compression. Piezo electric disks mounted on composite specimens were used as actuators and sensors. Three point bending fatigue tests were carried out on an intact composite laminate and a delaminated composite laminate with [06/904/06] orientation. Finite element analysis was performed to test the validity of SDC and NCM for fatigue damage.

  9. 14 CFR 29.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Damage Tolerance and Fatigue Evaluation of... Requirements Fatigue Evaluation § 29.573 Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft... practice, the applicant must do a fatigue evaluation in accordance with paragraph (e) of this section....

  10. 14 CFR 27.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Damage Tolerance and Fatigue Evaluation of... Requirements Fatigue Evaluation § 27.573 Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft... practice, the applicant must do a fatigue evaluation in accordance with paragraph (e) of this section....

  11. A damage mechanics approach to high temperature fatigue crack growth

    SciTech Connect

    Qian, Z.; Takezono, S.; Tao, K.

    1995-12-31

    A nonlocal damage constitutive model is developed for elasto-visco-plastic materials and is used to analyze fatigue crack growth at high temperature. In this model, no kinematic hardening rule is needed to account for the subsequent yielding and strain hardening behavior of the materials. A calculation method for nonlocal damage is introduced. The fatigue crack growth tests and the cyclic strain controlled fatigue tests are carried out at 723 K (450 C) on pure titanium. By means of FEM, the stress distribution near the crack tip, and the relationships between the crack growth rate dl/dN and some mechanical parameters, such as the crack tip opening displacement (CTOD), and the range of viscoplastic strain {Delta}{var_epsilon}{sub y}{sup vp} at the crack tip, are investigated. The mesh size dependence of these mechanical parameters in finite element analysis is discussed. The numerical results are given and compared with the experimental ones.

  12. On the Use of Infrared Thermography for Analysis of Fatigue Damage in Ti6Al4V-Welded Joints

    NASA Astrophysics Data System (ADS)

    Liu, Jing; Gao, Xiao-Long; Zhang, Lin-Jie; Zhang, Jian-Xun

    2014-08-01

    The present work is aimed at comparatively studying fatigue damage evolution of a pulsed Nd:YAG laser beam-welded (LBW) joint and the base metal (BM) of Ti6Al4V alloy subjected to cyclic loading. To reveal crack nucleation and propagation during the fatigue process, in situ fatigue was generated using infrared measurement methods. The results indicate that the rate of damage accumulated in the LBW joint was higher than in the BM specimens during a fatigue test, which decreased the fatigue life of the LBW joint. This observation is attributable to the LBW joint fusion zone microstructure, which has a higher void nucleation and growth rate compared with the BM microstructure.

  13. 77 FR 55105 - Aging Airplane Program: Widespread Fatigue Damage; Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-07

    ... establish a limit of validity of the engineering data that supports the structural maintenance program... tables. This document corrects the errors in those tables. DATES: This corrective action becomes... entitled ``Aging Airplane Program: Widespread Fatigue Damage'' (77 FR 30877), which corrected a final...

  14. Temporal Effect of In Vivo Tendon Fatigue Loading on the Apoptotic Response Explained in the Context of Number of Fatigue Loading Cycles and Initial Damage Parameters

    PubMed Central

    Andarawis-Puri, Nelly; Philip, Anaya; Laudier, Damien; Schaffler, Mitchell B.; Flatow, Evan L.

    2014-01-01

    Accumulation of damage is a leading factor in the development of tendinopathy. Apoptosis has been implicated in tendinopathy, but the biological mechanisms responsible for initiation and progression of these injuries are poorly understood. We assessed the relationship between initial induced damage and apoptotic activity 3 and 7 days after fatigue loading. We hypothesized that greater apoptotic activity (i) will be associated with greater induced damage and higher number of fatigue loading cycles, and (ii) will be higher at 7 than at 3 days after loading. Left patellar tendons were fatigue loaded for either 100 or 7,200 cycles. Diagnostic tests were applied before and after fatigue loading to determine the effect of fatigue loading on hysteresis, elongation, and loading and unloading stiffness (damage parameters). Cleaved Caspase-3 staining was used to identify and calculate the percent apoptosis in the patellar tendon. While no difference in apoptotic activity occurred between the 100 and 7,200 cycle groups, greater apoptotic activity was associated with greater induced damage. Apoptotic activity was higher at 7 than 3 days after loading. We expect that the decreasing number of healthy cells that can repair the induced damage in the tendon predispose it to further injury. PMID:24838769

  15. The use of ultrasonic signals and optical method to estimate the damage of materials after fatigue loading

    NASA Astrophysics Data System (ADS)

    Mishakin, V. V.; Mitenkov, F. M.; Klyushnikov, V. A.; Danilova, N. V.

    2010-12-01

    The influence of fatigue load of steels on parameters of ultrasonic and microplastic characteristics has been studied. A phenomenological theory, which connects process of damage accumulation (before appearance of crack) under fatigue loading with acoustic parameters and microplastic parameters, has been developed. Experimental studies showed that the combination of nondestructive methods of control (acoustical and optical) allows one to estimate the state of materials at an early stage of destruction in both low-cycle and high-cycle areas.

  16. Fatigue Damage Mechanisms in Advanced Hybrid Titanium Composite Laminates

    NASA Technical Reports Server (NTRS)

    Johnson, W. Steven; Rhymer, Donald W.; St.Clair, Terry L. (Technical Monitor)

    2000-01-01

    Hybrid Titanium Composite Laminates (HTCL) are a type of hybrid composite laminate with promise for high-speed aerospace applications, specifically designed for improved damage tolerance and strength at high-temperature (350 F, 177 C). However, in previous testing, HTCL demonstrated a propensity to excessive delamination at the titanium/PMC interface following titanium cracking. An advanced HTCL has been constructed with an emphasis on strengthening this interface, combining a PETI-5/IM7 PMC with Ti-15-3 foils prepared with an alkaline-perborate surface treatment. This paper discusses how the fatigue capabilities of the "advanced" HTCL compare to the first generation HTCL which was not modified for interface optimization, in both tension-tension (R = 0.1) and tension-compression (R=-0.2). The advanced HTCL under did not demonstrate a significant improvement in fatigue life, in either tension-tension or tension-compression loading. However, the advanced HTCL proved much more damage tolerant. The R = 0.1 tests revealed the advanced HTCL to increase the fatigue life following initial titanium ply damage up to 10X that of the initial HTCL at certain stress levels. The damage progression following the initial ply damage demonstrated the effect of the strengthened PMC/titanium interface. Acetate film replication of the advanced HTCL edges showed a propensity for some fibers in the adjacent PMC layers to fail at the point of titanium crack formation, suppressing delamination at the Ti/PMC interface. The inspection of failure surfaces validated these findings, revealing PMC fibers bonded to the majority of the titanium surfaces. Tension compression fatigue (R = -0.2) demonstrated the same trends in cycles between initial damage and failure, damage progression, and failure surfaces. Moreover, in possessing a higher resistance to delamination, the advanced HTCL did not exhibit buckling following initial titanium ply cracking under compression unlike the initial HTCL.

  17. Sliding contact fatigue damage in layered ceramic structures.

    PubMed

    Kim, J-W; Kim, J-H; Thompson, V P; Zhang, Y

    2007-11-01

    Porcelain-veneered restorations often chip and fracture from repeated occlusal loading, making fatigue studies relevant. Most fatigue studies are limited to uni-axial loading without sliding motion. We hypothesized that bi-axial loading (contact-load-slide-liftoff, simulating a masticatory cycle), as compared with uni-axial loading, accelerates the fatigue of layered ceramics. Monolithic glass plates were epoxy-joined to polycarbonate substrates as a transparent model for an all-ceramic crown on dentin. Uni-and bi-axial cyclic contact was applied through a hard sphere in water, by means of a mouth-motion simulator apparatus. The uni-axial (contact-load-hold-liftoff) and traditional R-ratio fatigue (indenter never leaves the specimen surface) produced similar lifespans, while bi-axial fatigue was more severe. The accelerated crack growth rate in bi-axial fatigue is attributed to enhanced tensile stresses at the trailing edges of a moving indenter. Fracture mechanics descriptions for damage evolution in brittle materials loaded repeatedly with a sliding sphere are provided. Clinical relevance is addressed.

  18. Damage accumulation in neon implanted silicon

    SciTech Connect

    Oliviero, E.; Peripolli, S.; Amaral, L.; Fichtner, P. F. P.; Beaufort, M. F.; Barbot, J. F.; Donnelly, S. E.

    2006-08-15

    Damage accumulation in neon-implanted silicon with fluences ranging from 5x10{sup 14} to 5x10{sup 16} Ne cm{sup -2} has been studied in detail. As-implanted and annealed samples were investigated by Rutherford backscattering spectrometry under channeling conditions and by transmission electron microscopy in order to quantify and characterize the lattice damage. Wavelength dispersive spectrometry was used to obtain the relative neon content stored in the matrix. Implantation at room temperature leads to the amorphization of the silicon while a high density of nanosized bubbles is observed all along the ion distribution, forming a uniform and continuous layer for implantation temperatures higher than 250 deg.C. Clusters of interstitial defects are also present in the deeper part of the layer corresponding to the end of range of ions. After annealing, the samples implanted at temperatures below 250 deg.C present a polycrystalline structure with blisters at the surface while in the other samples coarsening of bubbles occurs and nanocavities are formed together with extended defects identified as (311) defects. The results are discussed in comparison to the case of helium-implanted silicon and in the light of radiation-enhanced diffusion.

  19. Inspecting for widespread fatigue damage: Is partial debonding the key?

    NASA Technical Reports Server (NTRS)

    Brewer, John

    1994-01-01

    Experimental and analytical results indicate that cracks can initiate, grow, and coalesce more rapidly in fuselage lap joints that have experienced partial or complete debonding. Computational analysis in this paper shows that stress concentrations and stress intensity factors at the rivet holes are far less severe when the bond is intact. Debonding hastens the initiation of widespread fatigue cracks and significantly increases crack growth rate. Thus, debonded regions serve as "breeding grounds" for widespread fatigue damage. Therefore, the effectiveness of lap joint inspection programs may be enhanced if detailed inspections are focused on areas in which debonding has been detected.

  20. Applications of a new magnetic monitoring technique to in situ evaluation of fatigue damage in ferrous components

    SciTech Connect

    Jiles, D.C.; Biner, S.B.; Govindaraju, M.R.; Chen, Z.J.

    1994-06-01

    This project consisted of research into the use of magnetic inspection methods for the estimation of fatigue life of nuclear pressure vessel steel. Estimating the mechanical and magnetic properties of ferromagnetic materials are closely interrelated, therefore, measurements of magnetic properties could be used to monitor the evolution of fatigue damage in specimens subjected to cyclic loading. Results have shown that is possible to monitor the fatigue damage nondestructively by magnetic techniques. For example, in load-controlled high-cycle fatigue tests, it has been found that the plastic strain and coercivity accumulate logarithmically during the fatigue process. Thus a quantitative relationship between coercivity and the number of fatigue cycles could be established based on two empirical coefficients, which can be determined from the test conditions and material properties. Also it was found that prediction of the onset of fatigue failure in steels was possible under certain conditions. In strain-controlled low cycle fatigue, critical changes in Barkhausen emissions, coercivity and hysteresis loss occurred in the last ten to twenty percent of fatigue life.

  1. Fatigue damage prognosis using affine arithmetic

    NASA Astrophysics Data System (ADS)

    Gbaguidi, Audrey; Kim, Daewon

    2014-02-01

    Among the essential steps to be taken in structural health monitoring systems, damage prognosis would be the field that is least investigated due to the complexity of the uncertainties. This paper presents the possibility of using Affine Arithmetic for uncertainty propagation of crack damage in damage prognosis. The structures examined are thin rectangular plates made of titanium alloys with central mode I cracks and a composite plate with an internal delamination caused by mixed mode I and II fracture modes, under a harmonic uniaxial loading condition. The model-based method for crack growth rates are considered using the Paris Erdogan law model for the isotropic plates and the delamination growth law model proposed by Kardomateas for the composite plate. The parameters for both models are randomly taken and their uncertainties are considered as defined by an interval instead of a probability distribution. A Monte Carlo method is also applied to check whether Affine Arithmetic (AA) leads to tight bounds on the lifetime of the structure.

  2. Linking asphalt binder fatigue to asphalt mixture fatigue performance using viscoelastic continuum damage modeling

    NASA Astrophysics Data System (ADS)

    Safaei, Farinaz; Castorena, Cassie; Kim, Y. Richard

    2016-08-01

    Fatigue cracking is a major form of distress in asphalt pavements. Asphalt binder is the weakest asphalt concrete constituent and, thus, plays a critical role in determining the fatigue resistance of pavements. Therefore, the ability to characterize and model the inherent fatigue performance of an asphalt binder is a necessary first step to design mixtures and pavements that are not susceptible to premature fatigue failure. The simplified viscoelastic continuum damage (S-VECD) model has been used successfully by researchers to predict the damage evolution in asphalt mixtures for various traffic and climatic conditions using limited uniaxial test data. In this study, the S-VECD model, developed for asphalt mixtures, is adapted for asphalt binders tested under cyclic torsion in a dynamic shear rheometer. Derivation of the model framework is presented. The model is verified by producing damage characteristic curves that are both temperature- and loading history-independent based on time sweep tests, given that the effects of plasticity and adhesion loss on the material behavior are minimal. The applicability of the S-VECD model to the accelerated loading that is inherent of the linear amplitude sweep test is demonstrated, which reveals reasonable performance predictions, but with some loss in accuracy compared to time sweep tests due to the confounding effects of nonlinearity imposed by the high strain amplitudes included in the test. The asphalt binder S-VECD model is validated through comparisons to asphalt mixture S-VECD model results derived from cyclic direct tension tests and Accelerated Loading Facility performance tests. The results demonstrate good agreement between the asphalt binder and mixture test results and pavement performance, indicating that the developed model framework is able to capture the asphalt binder's contribution to mixture fatigue and pavement fatigue cracking performance.

  3. Thermal fatigue damage of Cu-Cr-Zr alloys

    NASA Astrophysics Data System (ADS)

    Chatterjee, Arya; Mitra, R.; Chakraborty, A. K.; Rotti, C.; Ray, K. K.

    2013-11-01

    The primary aim of this investigation is to examine thermal fatigue damage (TFD) in Cu-Cr-Zr alloys used in High Heat Flux components of Tokamak and its subsystems. Thermal fatigue experiments have been carried out between 290 °C and 30 °C, which is analogous to the condition of service application on two Cu-Cr-Zr alloys having different aging treatments. The extents of TFD have been examined by standard measurements of electrical conductivity, lattice strain, residual stress and dynamic elastic modulus, supplemented by characterizations of microstructure and determination of hardness and tensile properties. The results lead to infer that the relative amounts of damage are different in the two alloys which are further dependent on their aging conditions; the reasons for the observed difference have been explained. The operative mechanisms of TFD are revealed to be as formation and subsequent coalescence of microvoids, and/or initiation and growth of microcracks.

  4. Application of cyclic damage accumulation life prediction model to high temperature components

    NASA Technical Reports Server (NTRS)

    Nelson, Richard S.

    1989-01-01

    A high temperature, low cycle fatigue life prediction method was developed. This method, Cyclic Damage Accumulation (CDA), was developed for use in predicting the crack initiation lifetime of gas turbine engine materials, but it can be applied to other materials as well. The method is designed to account for the effects on creep-fatigue life of complex loading such as thermomechanical fatigue, hold periods, waveshapes, mean stresses, multiaxiality, cumulative damage, coatings, and environmental attack. Several features of this model were developed to make it practical for application to actual component analysis, such as the ability to handle nonisothermal loading (including TMF), arbitrary cycle paths, and multiple damage modes. The CDA life prediction model was derived from extensive specimen tests conducted on cast nickel-base superalloy B1900 + Hf. These included both monotonic tests (tensile and creep) and strain-controlled fatigue experiments (uniaxial, biaxial, TMF, mixed creep-fatigue, and controlled mean stress). Additional specimen tests were conducted on wrought INCO 718 to verify the applicability of the final CDA model to other high-temperature alloys. The model will be available to potential users in the near future in the form of a FORTRAN-77 computer program.

  5. Nonlinear ultrasound modelling and validation of fatigue damage

    NASA Astrophysics Data System (ADS)

    Fierro, G. P. Malfense; Ciampa, F.; Ginzburg, D.; Onder, E.; Meo, M.

    2015-05-01

    Nonlinear ultrasound techniques have shown greater sensitivity to microcracks and they can be used to detect structural damages at their early stages. However, there is still a lack of numerical models available in commercial finite element analysis (FEA) tools that are able to simulate the interaction of elastic waves with the materials nonlinear behaviour. In this study, a nonlinear constitutive material model was developed to predict the structural response under continuous harmonic excitation of a fatigued isotropic sample that showed anharmonic effects. Particularly, by means of Landau's theory and Kelvin tensorial representation, this model provided an understanding of the elastic nonlinear phenomena such as the second harmonic generation in three-dimensional solid media. The numerical scheme was implemented and evaluated using a commercially available FEA software LS-DYNA, and it showed a good numerical characterisation of the second harmonic amplitude generated by the damaged region known as the nonlinear response area (NRA). Since this process requires only the experimental second-order nonlinear parameter and rough damage size estimation as an input, it does not need any baseline testing with the undamaged structure or any dynamic modelling of the fatigue crack growth. To validate this numerical model, the second-order nonlinear parameter was experimentally evaluated at various points over the fatigue life of an aluminium (AA6082-T6) coupon and the crack propagation was measured using an optical microscope. A good correlation was achieved between the experimental set-up and the nonlinear constitutive model.

  6. Fatigue Crack Growth Database for Damage Tolerance Analysis

    NASA Technical Reports Server (NTRS)

    Forman, R. G.; Shivakumar, V.; Cardinal, J. W.; Williams, L. C.; McKeighan, P. C.

    2005-01-01

    The objective of this project was to begin the process of developing a fatigue crack growth database (FCGD) of metallic materials for use in damage tolerance analysis of aircraft structure. For this initial effort, crack growth rate data in the NASGRO (Registered trademark) database, the United States Air Force Damage Tolerant Design Handbook, and other publicly available sources were examined and used to develop a database that characterizes crack growth behavior for specific applications (materials). The focus of this effort was on materials for general commercial aircraft applications, including large transport airplanes, small transport commuter airplanes, general aviation airplanes, and rotorcraft. The end products of this project are the FCGD software and this report. The specific goal of this effort was to present fatigue crack growth data in three usable formats: (1) NASGRO equation parameters, (2) Walker equation parameters, and (3) tabular data points. The development of this FCGD will begin the process of developing a consistent set of standard fatigue crack growth material properties. It is envisioned that the end product of the process will be a general repository for credible and well-documented fracture properties that may be used as a default standard in damage tolerance analyses.

  7. Fatigue-induced damage in glutaraldehyde-preserved heart valve tissue.

    PubMed

    Broom, N D

    1978-08-01

    Glutaraldehyde-preserved porcine mitral leaflet tissue has been subjected to extended accelerated fatigue loading in Ringer's solution containing 0.15% glutaraldehyde. Five tissue test pieces were subjected to cyclic tensile stresses of 50 and 200 Gm. per square millimeter and to 300 million to 800 million accumulated fatigue cycles. Tissue disruption occurred in each of the fatigued test pieces. Tensile loading, apart from reducing the acuteness of the collagen waveform and thereby decreasing tissue compliance, does not contribute significantly to the disruption process nor its rate of occurrence. Compressive flexure occurring during the unloading half of the fatigue cycle, however, does induce damage in the tissue. Mechanisms involved in the disruptive processes have been identified by conducting simultaneous morphologic and stress/strain observations on both the fatigued and unfatigued tissues in their wet functional condition. This vulnerability of the preserved tissue to compressive flexure could well affect the long-term durability of the glutaraldehyde-preserved heterograft valve, and this possibility is discussed in relation to the clinical use of these valves.

  8. 14 CFR 23.574 - Metallic damage tolerance and fatigue evaluation of commuter category airplanes.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Metallic damage tolerance and fatigue... COMMUTER CATEGORY AIRPLANES Structure Fatigue Evaluation § 23.574 Metallic damage tolerance and fatigue... evaluation of the strength, detail design, and fabrication must show that catastrophic failure due to...

  9. Fatigue Damage Spectrum calculation in a Mission Synthesis procedure for Sine-on-Random excitations

    NASA Astrophysics Data System (ADS)

    Angeli, Andrea; Cornelis, Bram; Troncossi, Marco

    2016-09-01

    In many real-life environments, certain mechanical and electronic components may be subjected to Sine-on-Random vibrations, i.e. excitations composed of random vibrations superimposed on deterministic (sinusoidal) contributions, in particular sine tones due to some rotating parts of the system (e.g. helicopters, engine-mounted components,...). These components must be designed to withstand the fatigue damage induced by the “composed” vibration environment, and qualification tests are advisable for the most critical ones. In the case of an accelerated qualification test, a proper test tailoring which starts from the real environment (measured vibration signals) and which preserves not only the accumulated fatigue damage but also the “nature” of the excitation (i.e. sinusoidal components plus random process) is important to obtain reliable results. In this paper, the classic time domain approach is taken as a reference for the comparison of different methods for the Fatigue Damage Spectrum (FDS) calculation in case of Sine-on-Random vibration environments. Then, a methodology to compute a Sine-on-Random specification based on a mission FDS is proposed.

  10. Modeling of thermo-mechanical fatigue and damage in shape memory alloy axial actuators

    NASA Astrophysics Data System (ADS)

    Wheeler, Robert W.; Hartl, Darren J.; Chemisky, Yves; Lagoudas, Dimitris C.

    2015-04-01

    The aerospace, automotive, and energy industries have seen the potential benefits of using shape memory alloys (SMAs) as solid state actuators. Thus far, however, these actuators are generally limited to non-critical components or over-designed due to a lack of understanding regarding how SMAs undergo thermomechanical or actuation fatigue and the inability to accurately predict failure in an actuator during use. The purpose of this study was to characterize the actuation fatigue response of Nickel-Titanium-Hafnium (NiTiHf) axial actuators and, in turn, use this characterization to predict failure and monitor damage in dogbone actuators undergoing various thermomechanical loading paths. Calibration data was collected from constant load, full cycle tests ranging from 200-600MPa. Subsequently, actuator lifetimes were predicted for four additional loading paths. These loading paths consisted of linearly varying load with full transformation (300-500MPa) and step loads which transition from zero stress to 300-400MPa at various martensitic volume fractions. Thermal cycling was achieved via resistive heating and convective cooling and was controlled via a state machine developed in LabVIEW. A previously developed fatigue damage model, which is formulated such that the damage accumulation rate is general in terms of its dependence on current and local stress and actuation strain states, was utilized. This form allows the model to be utilized for specimens undergoing complex loading paths. Agreement between experiments and simulations is discussed.

  11. The effect of yield strength and ductility to fatigue damage

    NASA Technical Reports Server (NTRS)

    Yeh, H. Y.

    1973-01-01

    The cumulative damage of aluminium alloys with different yield strength and various ductility due to seismic loads was studied. The responses of an idealized beam with a centered mass at one end and fixed at the other end to El Centro's and Taft's earthquakes are computed by assuming that the alloys are perfectly elastoplastic materials and by using numerical technique. Consequently, the corresponding residual plastic strain can be obtained from the stress-strain relationship. The revised Palmgren-Miner cumulative damage theorem is utilized to calculate the fatigue damage. The numerical results show that in certain cases, the high ductility materials are more resistant to seismic loads than the high yield strength materials. The results also show that if a structure collapse during the earthquake, the collapse always occurs in the very early stage.

  12. 77 FR 4890 - Damage Tolerance and Fatigue Evaluation for Composite Rotorcraft Structures, and Damage Tolerance...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-01

    ... Composite Rotorcraft Structures'' (76 FR 74655), published December 1, 2011, and ``Damage Tolerance and... Tolerance and Fatigue Evaluation for Composite Rotorcraft Structures'' (76 FR 74655). On December 2, 2011... Structures'' (76 FR 75435). In the ``Composite Rotorcraft Structures'' final rule, the FAA amended...

  13. Influence of oxidation treatment on fatigue and fatigue-induced damage of commercially pure titanium.

    PubMed

    Leinenbach, C; Eifler, D

    2009-09-01

    In this investigation, the cyclic deformation behaviour of commercially pure titanium was characterized in axial stress controlled constant amplitude and load increase tests, as well as in rotating bending tests. The influence of different clinically relevant surface treatments (polishing, thermal and anodic oxidizing) on the fatigue behaviour was investigated. All tests were realized in oxygen-saturated Ringer's solution. The cyclic deformation behaviour was characterized by mechanical hysteresis measurements. In addition, the change of the free corrosion potential and the corrosion current during the fatigue tests in simulated physiological media indicated such types of surface damage as slip bands, microcracks and oxide film ablation. Microstructural changes on the specimen surfaces were examined by scanning electron microscopy. PMID:19394905

  14. Estimation of early fatigue damage in heat treated En-8 grade steel

    NASA Astrophysics Data System (ADS)

    Talukdar, P.; Sen, S. K.; Ghosh, A. K.

    1998-08-01

    Generally, the failure of major machinery parts is due to fatigue damage. Because of the structural inhomogeneity of metals, fatigue damage may sometimes occur significantly below the yield strength of the material due to microplastic deformation at low stress levels. Commercial En-8 grade steel (widely used for making secondary metalworking products) was used to estimate the fatigue damage response during cyclic loading nearer to the fatigue endurance limit. Estimation of fatigue damage was carried out with the aid of a nondestructive testing (NDT) method, that is, Elastosonic measurement of fatigue damping coefficient and slope of fatigue damping curves. Results indicate that fatigue damage increases in annealed En-8 steel with an increase in peak stress and with an increase in the number of cycles. However, for hardened and tempered En-8 steel, experimental results may not provide a true indication of fatigue damage during fatigue loading nearer to the endurance limit, most likely due to the more homogeneous structure. Generally, fatigue failure occurs in this grade of steel due to microcrack generation in the cementite of the pearlite phase of annealed steel.

  15. Residual strength and crack propagation tests on C-130 airplane center wings with service-imposed fatigue damage

    NASA Technical Reports Server (NTRS)

    Snider, H. L.; Reeder, F. L.; Dirkin, W. J.

    1972-01-01

    Fourteen C-130 airplane center wings, each containing service-imposed fatigue damage resulting from 4000 to 13,000 accumulated flight hours, were tested to determine their fatigue crack propagation and static residual strength characteristics. Eight wings were subjected to a two-step constant amplitude fatigue test prior to static testing. Cracks up to 30 inches long were generated in these tests. Residual static strengths of these wings ranged from 56 to 87 percent of limit load. The remaining six wings containing cracks up to 4 inches long were statically tested as received from field service. Residual static strengths of these wings ranged from 98 to 117 percent of limit load. Damage-tolerant structural design features such as fastener holes, stringers, doublers around door cutouts, and spanwise panel splices proved to be effective in retarding crack propagation.

  16. Fatigue and Mechanical Damage Propagation in Automotive PEM Fuel Cells

    NASA Astrophysics Data System (ADS)

    Banan, Roshanak

    Polymer electrolyte membrane (PEM) fuel cells are generally exposed to high magnitude road-induced vibrations and impact loads, frequent humidity-temperature loading cycles, and freeze/thaw stresses when employed in automotive applications. The resultant mechanical stresses can play a significant role in the evolution of mechanical defects in the membrane electrode assembly (MEA). The focus of this research is to investigate fatigue challenges due to humidity-temperature (hygrothermal) cycles and vibrations and their effects on damage evolution in PEM fuel cells. To achieve this goal, this thesis is divided into three parts that provide insight into damage propagation in the MEA under i) hygrothermal cycles, ii) external applied vibrations, and iii) a combination of both to simulate realistic automotive conditions. A finite element damage model based on cohesive zone theory was developed to simulate the propagation of micro-scale defects (cracks and delaminations) in the MEA under fuel cell operating conditions. It was found that the micro-defects can propagate to critical states under start-up and shut-down cycles, prior to reaching the desired lifespan of the fuel cell. The simultaneous presence of hygrothermal cycles and vibrations severely intensified damage propagation and resulted in considerably large defects within 75% of the fuel cell life expectancy. However, the order of generated damage was found to be larger under hygrothermal cycles than vibrations. Under hygrothermal cycles, membrane crack propagation was more severe compared to delamination propagation. Conversely, the degrading influence of vibrations was more significant on delaminations. The presence of an anode/cathode channel offset under the combined loadings lead to a 2.5-fold increase in the delamination length compared to the aligned-channel case. The developed model can be used to investigate the damage behaviour of current materials employed in fuel cells as well as to evaluate the

  17. Investigation of Gear and Bearing Fatigue Damage Using Debris Particle Distributions

    NASA Technical Reports Server (NTRS)

    Dempsey, Paula J.; Lewicki, David G.; Decker, Harry J.

    2004-01-01

    A diagnostic tool was developed for detecting fatigue damage to spur gears, spiral bevel gears, and rolling element bearings. This diagnostic tool was developed and evaluated experimentally by collecting oil debris data from fatigue tests performed in the NASA Glenn Spur Gear Fatigue Rig, Spiral Bevel Gear Test Facility, and the 500hp Helicopter Transmission Test Stand. During each test, data from an online, in-line, inductance type oil debris sensor was monitored and recorded for the occurrence of pitting damage. Results indicate oil debris alone cannot discriminate between bearing and gear fatigue damage.

  18. A study of cumulative fatigue damage in titanium 6Al-4V alloy

    NASA Technical Reports Server (NTRS)

    Jeelani, S.; Ghebremedhin, S.; Musial, M.

    1986-01-01

    Experimental data were obtained using titanium 6Al-4V alloy under stress ratios of -1, 0, and negative infinity. A study of cumulative fatigue damage using Miner's (1945) and Kramer's (1974) equations for stress ratios of -1 and 0 for low-high, low-high mixed, high-low, and high-low mixed stress sequences has revealed close agreement between the theoretical and experimental values of fatigue damage and fatigue life. Kramer's equation predicts less conservative and more realistic cumulative fatigue damage than does the popularly used Miner's rule.

  19. Atomistic simulation of damage accumulation and amorphization in Ge

    SciTech Connect

    Gomez-Selles, Jose L. Martin-Bragado, Ignacio; Claverie, Alain; Benistant, Francis

    2015-02-07

    Damage accumulation and amorphization mechanisms by means of ion implantation in Ge are studied using Kinetic Monte Carlo and Binary Collision Approximation techniques. Such mechanisms are investigated through different stages of damage accumulation taking place in the implantation process: from point defect generation and cluster formation up to full amorphization of Ge layers. We propose a damage concentration amorphization threshold for Ge of ∼1.3 × 10{sup 22} cm{sup −3} which is independent on the implantation conditions. Recombination energy barriers depending on amorphous pocket sizes are provided. This leads to an explanation of the reported distinct behavior of the damage generated by different ions. We have also observed that the dissolution of clusters plays an important role for relatively high temperatures and fluences. The model is able to explain and predict different damage generation regimes, amount of generated damage, and extension of amorphous layers in Ge for different ions and implantation conditions.

  20. 77 FR 30877 - Aging Airplane Program: Widespread Fatigue Damage; Technical Amendment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-24

    ... Airplane Program: Widespread Fatigue Damage,'' (75 FR 69746). In that final rule the FAA revised the... actions (see Damage Tolerance Data for Repairs and Alterations, 72 FR 70486). Change to Table 1 of Sec... Administration 14 CFR Parts 26, 121, and 129 RIN 2120-AI05 Aging Airplane Program: Widespread Fatigue...

  1. 76 FR 74655 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-01

    ... static strength of composite rotorcraft structures using a damage tolerance evaluation, or a fatigue... also harmonize this standard with international standards for evaluating the fatigue strength of normal... damage and loading conditions. This rule addresses the unique characteristics of composite materials...

  2. 14 CFR 23.574 - Metallic damage tolerance and fatigue evaluation of commuter category airplanes.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... evaluation of commuter category airplanes. 23.574 Section 23.574 Aeronautics and Space FEDERAL AVIATION... COMMUTER CATEGORY AIRPLANES Structure Fatigue Evaluation § 23.574 Metallic damage tolerance and fatigue evaluation of commuter category airplanes. For commuter category airplanes— (a) Metallic damage tolerance....

  3. 14 CFR 23.574 - Metallic damage tolerance and fatigue evaluation of commuter category airplanes.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... evaluation of commuter category airplanes. 23.574 Section 23.574 Aeronautics and Space FEDERAL AVIATION... COMMUTER CATEGORY AIRPLANES Structure Fatigue Evaluation § 23.574 Metallic damage tolerance and fatigue evaluation of commuter category airplanes. For commuter category airplanes— (a) Metallic damage tolerance....

  4. Damage accumulation in closed cross-section, laminated, composite structures

    NASA Technical Reports Server (NTRS)

    Bucinell, Ronald B.

    1996-01-01

    The need for safe, lightweight, less expensive, and more reliable launch vehicle components is being driven by the competitiveness of the commercial launch market. The United States has lost 2/3 of the commercial lunch market to Europe. As low cost Russian and Chinese vehicles become available, the US market share could be reduced even further. This international climate is driving the Single Stage To Orbit (SSTO) program at NASA. The goal of the SSTO program is to radically reduce the cost of safe, routine transportation to and from space with a totally reusable launch vehicle designed for low-cost aircraft-like operations. Achieving this goal will require more efficient uses of materials. Composite materials can provide this program with the material and structural efficiencies needed to stay competitive in the international launch market place. In satellite systems the high specific properties, design flexibility, improved corrosion and wear resistance, increased fatigue life, and low coefficient of thermal expansion that are characteristic of composite materials can all be used to improve the overall satellite performance. Some of the satellites that may be able to take advantage of these performance characteristics are the Tethered Satellite Systems (TOSCIFER, AIRSEDS, TSS2, SEDS1, and SEDS2), AXAF, GRO, and the next generation Hubble Space Telescope. These materials can also be utilized in projects at the NASAIMSFC Space Optics Technology and System Center of Excellence. The successful implementation of composite materials requires accurate performance characterization. Materials characterization data for composite materials is typically generated using flat coupons of finite width. At the free edge of these coupons the stress state is exacerbated by the presence of stiffness and geometric discontinuities. The exacerbated stress state has been shown to dominate the damage accumulation in these materials and to have a profound affect on the material constants

  5. Fatigue damage in cross-ply titanium metal matrix composites containing center holes

    NASA Technical Reports Server (NTRS)

    Bakuckas, J. G., Jr.; Johnson, W. S.; Bigelow, C. A.

    1992-01-01

    The development of fatigue damage in (0/90) sub SCS-6/TI-15-3 laminates containing center holes was studied. Stress levels required for crack initiation in the matrix were predicted using an effective strain parameter and compared to experimental results. Damage progression was monitored at various stages of fatigue loading. In general, a saturated state of damage consisting of matrix cracks and fiber matrix debonding was obtained which reduced the composite modulus. Matrix cracks were bridged by the 0 deg fibers. The fatigue limit (stress causing catastrophic fracture of the laminates) was also determined. The static and post fatigue residual strengths were accurately predicted using a three dimensional elastic-plastic finite element analysis. The matrix damage that occurred during fatigue loading significantly reduced the notched strength.

  6. Cumulative creep-fatigue damage evolution in an austenitic stainless steel

    NASA Technical Reports Server (NTRS)

    Mcgaw, Michael A.

    1992-01-01

    A model of cumulative creep-fatigue damage has been developed which is based on the use of damage curve equations to describe the evolution of creep-fatigue damage for four basic creep-fatigue cycle types. These cycle types correspond to the four fundamental cycles of the Strain Range Partitioning Life Prediction approach of Manson, Halford, and Hirschberg. A concept referred to as Damage Coupling is introduced to analytically account for the differences in the nature of the damage introduced by each cycle type. For application of this model, the cumulative creep-fatigue damage behavior of type 316 stainless steel at 816 C has been experimentally established for the two-level loading cases involving fatigue and creep-fatigue, in various permutations. The tests were conducted such that the lower life (high strain) cycling was applied first, for a controlled number of cycles, and the higher life (lower strain) cycling was conducted at the second level, to failure. The proposed model correlated the majority of the observed cumulative creep-fatigue data.

  7. Microstructure: Property correlation. [multiaxial fatigue damage evolution in waspaloy

    NASA Technical Reports Server (NTRS)

    Jayaraman, N.

    1990-01-01

    Strain controlled torsional and biaxial (tension-torsion) low cycle fatigue behavior of Waspaloy was studied at room temperature as a function of heat treatment. Biaxial tests were conducted under proportional (when the axial and torsional strain cycles are in-phase) and non-proportional (when the axial and torsional strain cycles are 90 deg out-of-phase) cyclic conditions. The deformation behavior under these different cyclic conditions were evaluated by slip trace analysis. For this, a Schmidt-type factor was defined for multiaxial loading conditions and it was shown that when the slip deformation is predominant, non-proportional cycles are more damaging than proportional or pure axial or torsional cycles. This was attributed to the fact that under non-proportional cyclic conditions, deformation was through multiple slip as opposed single slip for other loading conditions, which gave rise to increased hardening. The total life for a given test condition was found to be independent of heat treatment. This was interpreted as being due to the differences in the cycles to initiation and propagation of cracks.

  8. Fatigue crack growth spectrum simplification: Facilitation of on-board damage prognosis systems

    NASA Astrophysics Data System (ADS)

    Adler, Matthew Adam

    2009-12-01

    Better lifetime predictions of systems subjected to fatigue loading are needed in support of the optimization of the costs of life-cycle engineering. In particular, the climate is especially encouraging for the development of safer aircraft. One issue is that aircraft experience complex fatigue loading and current methods for the prediction of fatigue damage accumulation rely on intensive computational tools that are not currently carried onboard during flight. These tools rely on complex models that are made more difficult by the complicated load spectra themselves. This presents an overhead burden as offline analysis must be performed at an offsite facility. This architecture is thus unable to provide online, timely information for on-board use. The direct objective of this research was to facilitate the real-time fatigue damage assessments of on-board systems with a particular emphasis on aging aircraft. To achieve the objective, the goal of this research was to simplify flight spectra. Variable-amplitude spectra, in which the load changes on a cycle-by-cycle basis, cannot readily be supported by an onboard system because the models required to predict fatigue crack growth during variable-amplitude loading are too complicated. They are too complicated because variable-amplitude fatigue crack growth analysis must be performed on a cycle-by-cycle basis as no closed-form solution exists. This makes these calculations too time-consuming and requires impractical, heavy onboard systems or offsite facilities. The hypothesis is to replace a variable-amplitude spectrum with an equivalent constant-amplitude spectrum. The advantage is a dramatic reduction in the complexity of the problem so that damage predictions can be made onboard by simple, fast calculations in real-time without the need to add additional weight to the aircraft. The intent is to reduce the computational burden and facilitate on-board projection of damage evolution and prediction for the accurate

  9. Nonlinear ultrasonic measurements with EMATs for detecting pre-cracking fatigue damage

    NASA Astrophysics Data System (ADS)

    Cobb, A.; Capps, M.; Duffer, C.; Feiger, J.; Robinson, K.; Hollingshaus, B.

    2012-05-01

    This paper describes an approach for measuring material degradation using nonlinear acoustics. The importance of this measurement is that prior efforts have shown that the degree of acoustic nonlinearity increases as a function of fatigue damage accumulation. By exploiting this physical mechanism, there is the potential to develop methods for measuring the remaining life of critical components. The challenge with existing approaches for measuring acoustic nonlinearity is that primarily they have only been shown to be successful in a laboratory setting. This paper presents a potential approach for field measurement of acoustic nonlinearity that utilizes Rayleigh waves generated from electromagnetic acoustic transducers (EMATs). Rayleigh waves have unique advantages because the sound propagates along the surface, allowing for application on complex engineering structures. EMATs were used in place of traditional piezoelectric transducers because the sound is generated directly in the metallic structure, eliminating the need for sound coupling fluids that are a source of variability. Custom EMATs were developed and nonlinearity measurements were performed on 410 stainless steel specimens that were subjected to a fatigue process. Some experiments showed an increase in the acoustic nonlinearity of up to 500% compared to the unfatigued value. Other experiments had too much scatter and did not show this relationship consistently due to unanticipated challenges in producing repeatable measurements. Lessons learned from the project effort will be presented to potentially improve the repeatability of the measurement approach. If the scatter can be reduced, this EMAT-based technique could result in a field deployable prognosis tool.

  10. Fatigue Damage Evaluation of Friction Stir Spot Welded Cross-Tension Joints Under Repeated Two-Step Force Amplitudes

    NASA Astrophysics Data System (ADS)

    Joy-A-Ka, Sutep; Ogawa, Yuki; Akebono, Hiroyuki; Kato, Masahiko; Sugeta, Atsushi; Sun, Yufeng; Fujii, Hidetoshi

    2015-06-01

    This paper investigates an approach to evaluate the fatigue damage of FSSW cross-tension specimens under two-step force amplitude conditions. In fatigue tests with repeated two-step force amplitude, the fatigue limit of the welded joint disappeared. However, the fatigue damage evaluation using the modified Miner's rule erred too much on the side of safety, as the modified Miner's rule tends to overestimate the damage by applied forces below the fatigue limit. Thus, it was determined that, within the testing conditions used in this study, the fatigue damage evaluation using Haibach's method yielded an accurate evaluation. In the case where significant plastic deformation caused by the applied force occurred near the welded zone, the cumulative fatigue damage value based on Miner's rule was often larger than unity. Therefore, it is important to consider a cumulative damage estimation that takes into account the effect of pre-strain from the high force amplitude.

  11. Fatigue damage evaluation of plain woven carbon fiber reinforced plastic (CFRP) modified with MFC (micro-fibrillated cellulose) by thermo-elastic damage analysis (TDA)

    NASA Astrophysics Data System (ADS)

    Aoyama, Ryohei; Okubo, Kazuya; Fujii, Toru

    2013-04-01

    The aim of this study is to investigate characteristics of fatigue damage of CFRP modified with MFC by TDA under tensile cyclic loading. In this paper, fatigue life of CFRP modified with MFC was investigated under cyclic loading. Characteristics of fatigue damage of CFRP modified with MFC were evaluated by thermo-elastic damage analysis. Maximum improvement in fatigue life was also obtained under cyclic loading when epoxy matrix was enhanced with 0.3wt% of MFC as well as under static loading. Result of TDA showed same tendency as the result of fatigue test, and the result of TDA well expressed the fatigue damage behavior of plain woven CFRP plate. Eventually, TDA was effective for clear understanding the degree of fatigue damage progression of CFRP modified with MFC.

  12. Damage mechanisms in bithermal and thermomechanical fatigue of Haynes 188

    NASA Technical Reports Server (NTRS)

    Kalluri, Sreeramesh; Halford, Gary R.

    1992-01-01

    Post failure fractographic and metallographic studies were conducted on Haynes 188 specimens fatigued under bithermal and thermomechanical loading conditions between 316 and 760 C. Bithermal fatigue specimens examined included those tested under high strain rate in-phase and out-phase, tensile creep in-phase, and compressive creep out-of-phase loading conditions. Specimens tested under in-phase and out-of-phase thermomechanical fatigue were also examined. The nature of failure mode (transgrandular versus intergranular), the topography of the fracture surface, and the roles of oxidation and metallurgical changes were studied for each type of bithermal and thermomechanical test.

  13. A coupled/uncoupled deformation and fatigue damage algorithm utilizing the finite element method

    NASA Technical Reports Server (NTRS)

    Wilt, Thomas E.; Arnold, Steven M.

    1994-01-01

    A fatigue damage computational algorithm utilizing a multiaxial, isothermal, continuum based fatigue damage model for unidirectional metal matrix composites has been implemented into the commercial finite element code MARC using MARC user subroutines. Damage is introduced into the finite element solution through the concept of effective stress which fully couples the fatigue damage calculations with the finite element deformation solution. An axisymmetric stress analysis was performed on a circumferentially reinforced ring, wherein both the matrix cladding and the composite core were assumed to behave elastic-perfectly plastic. The composite core behavior was represented using Hill's anisotropic continuum based plasticity model, and similarly, the matrix cladding was represented by an isotropic plasticity model. Results are presented in the form of S-N curves and damage distribution plots.

  14. Comparative study of approaches to assess damage in thermally fatigued Cusbnd Crsbnd Zr alloy

    NASA Astrophysics Data System (ADS)

    Chatterjee, Arya; Mitra, R.; Chakraborty, A. K.; Rotti, C.; Ray, K. K.

    2016-06-01

    For the first time the nature of response of thermal fatigue damage (TFD) in Cusbnd Crsbnd Zr alloys, considered for the High Heat Flux components of Tokamak and its subsystems in International Thermonuclear Experimental Reactor application has been studied. Temperature cycling between 290 °C and 30 °C, similar to the service condition, has been carried out on two differently aged Cusbnd Crsbnd Zr alloys. The TFD has been assessed by damage mechanics approach using damage parameters, and by surface characteristics. The damage parameters increase exponentially during initial fatigue cycles and saturates, whilst surface characteristics shows continuous increase with increase in thermal fatigue cycles. Damages are different in the aged alloys depending upon the aging conditions.

  15. Fatigue damage of steam turbine shaft at asynchronous connections of turbine generator to electrical network

    NASA Astrophysics Data System (ADS)

    Bovsunovsky, A. P.

    2015-07-01

    The investigations of cracks growth in the fractured turbine rotors point out at theirs fatigue nature. The main reason of turbine shafts fatigue damage is theirs periodical startups which are typical for steam turbines. Each startup of a turbine is accompanied by the connection of turbine generator to electrical network. During the connection because of the phase shift between the vector of electromotive force of turbine generator and the vector of supply-line voltage the short-term but powerful reactive shaft torque arises. This torque causes torsional vibrations and fatigue damage of turbine shafts of different intensity. Based on the 3D finite element model of turbine shaft of the steam turbine K-200-130 and the mechanical properties of rotor steel there was estimated the fatigue damage of the shaft at its torsional vibrations arising as a result of connection of turbine generator to electric network.

  16. Differential continuum damage mechanics models for creep and fatigue of unidirectional metal matrix composites

    NASA Technical Reports Server (NTRS)

    Arnold, S. M.; Kruch, S.

    1991-01-01

    Three multiaxial isothermal continuum damage mechanics models for creep, fatigue, and creep/fatigue interaction of a unidirectional metal matrix composite volume element are presented, only one of which will be discussed in depth. Each model is phenomenological and stress based, with varying degrees of complexity to accurately predict the initiation and propagation of intergranular and transgranular defects over a wide range of loading conditions. The development of these models is founded on the definition of an initially transversely isotropic fatigue limit surface, static fracture surface, normalized stress amplitude function and isochronous creep damage failure surface, from which both fatigue and creep damage evolutionary laws can be obtained. The anisotropy of each model is defined through physically meaningful invariants reflecting the local stress and material orientation. All three transversely isotropic models have been shown, when taken to their isotropic limit, to directly simplify to previously developed and validated creep and fatigue continuum damage theories. Results of a nondimensional parametric study illustrate (1) the flexibility of the present formulation when attempting to characterize a large class of composite materials, and (2) its ability to predict anticipated qualitative trends in the fatigue behavior of unidirectional metal matrix composites. Additionally, the potential for the inclusion of various micromechanical effects (e.g., fiber/matrix bond strength, fiber volume fraction, etc.), into the phenomenological anisotropic parameters is noted, as well as a detailed discussion regarding the necessary exploratory and characterization experiments needed to utilize the featured damage theories.

  17. Effect of Impact Damage on the Fatigue Response of TiAl Alloy-ABB-2

    NASA Technical Reports Server (NTRS)

    Draper, S. L.; Lerch, B. A.; Pereira, J. M.; Nathal, M. V.; Nazmy, M. Y.; Staubli, M.; Clemens, D. R.

    2001-01-01

    The ability of gamma-TiAl to withstand potential foreign or domestic object damage is a technical risk to the implementation of gamma-TiAl in low pressure turbine (LPT) blade applications. In the present study, the impact resistance of TiAl alloy ABB-2 was determined and compared to the impact resistance of Ti(48)Al(2)Nb(2)Cr. Specimens were impacted with four different impact conditions with impact energies ranging from 0.22 to 6.09 J. After impacting, the impact damage was characterized by crack lengths on both the front and backside of the impact. Due to the flat nature of gamma-TiAl's S-N (stress vs. cycles to failure) curve, step fatigue tests were used to determine the fatigue strength after impacting. Impact damage increased with increasing impact energy and led to a reduction in the fatigue strength of the alloy. For similar crack lengths, the fatigue strength of impacted ABB-2 was similar to the fatigue strength of impacted Ti(48)Al(2)Nb(2)Cr, even though the tensile properties of the two alloys are significantly different. Similar to Ti(48)Al(2)Nb(2)Cr, ABB-2 showed a classical mean stress dependence on fatigue strength. The fatigue strength of impacted ABB-2 could be accurately predicted using a threshold analysis.

  18. 14 CFR 23.574 - Metallic damage tolerance and fatigue evaluation of commuter category airplanes.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Metallic damage tolerance and fatigue evaluation of commuter category airplanes. 23.574 Section 23.574 Aeronautics and Space FEDERAL AVIATION..., corrosion, defects, or damage will be avoided throughout the operational life of the airplane....

  19. 14 CFR 23.574 - Metallic damage tolerance and fatigue evaluation of commuter category airplanes.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Metallic damage tolerance and fatigue evaluation of commuter category airplanes. 23.574 Section 23.574 Aeronautics and Space FEDERAL AVIATION..., corrosion, defects, or damage will be avoided throughout the operational life of the airplane....

  20. 14 CFR 25.571 - Damage-tolerance and fatigue evaluation of structure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... which could cause catastrophic failure of the airplane; and (iii) An analysis, supported by test.... Inspection thresholds for the following types of structure must be established based on crack growth analyses... locations and modes of damage due to fatigue, corrosion, or accidental damage. Repeated load and...

  1. A damage-based approach for the fatigue design of composite structures

    NASA Astrophysics Data System (ADS)

    Quaresimin, Marino

    2016-07-01

    The paper illustrates a fatigue design strategy, based on the physics of the damage evolution, under development by the Composite Group at DTG-University of Padova. After a brief introduction, where the motivations of the work are analysed, examples of damage mechanisms at the microscopic scale are discussed. Then the procedures for the quantitative description of these mechanisms are illustrated.

  2. The fatigue damage behavior of a single crystal superalloy

    NASA Technical Reports Server (NTRS)

    Mcgaw, Michael A.

    1988-01-01

    The uniaxial fatigue behavior of a single crystal superalloy, PWA 1480, is described. Both monotonic tensile and constant amplitude fatigue tests were conducted at room temperature, in an effort to assess the applicability of polycrystalline-based fatigue life prediction methods to a single crystal superalloy. The observed constant amplitude behavior correlated best using a stress-based life criterion. Nearly all specimens failed at surface or slightly subsurface microporosity; this is thought to be responsible for the unusually large amount of scatter in the test results. An additional term is developed in the stress-life equation for the purpose of accounting for the effect of microporosity on fatigue life. The form chosen is a function of the effective area of the failure-producing microporosity projected on a plane perpendicular to the loading axis, as well as the applied stress. This additional term correlated the data to within factors of two on life. Although speculative, extrapolation of the microporosity relation to zero micropore area indicates that approximately an order of magnitude improvement in fatigue life should result.

  3. Comparative study of radiation damage accumulation in Cu and Fe

    NASA Astrophysics Data System (ADS)

    Caturla, M. J.; Soneda, N.; Alonso, E.; Wirth, B. D.; Díaz de la Rubia, T.; Perlado, J. M.

    2000-01-01

    Bcc and fcc metals exhibit significant differences in behavior when exposed to neutron or heavy ion irradiation. Transmission electron microscopy (TEM) observations reveal that damage in the form of stacking fault tetrahedra (SFT) is visible in copper irradiated to very low doses, but that no damage is visible in iron irradiated to the same total dose. In order to understand and quantify this difference in behavior, we have simulated damage production and accumulation in fcc Cu and bcc Fe. We use 20 keV primary knock-on atoms (PKAs) at a homologous temperature of 0.25 of the melting point. The primary damage state was calculated using molecular dynamics (MD) with empirical, embedded-atom interatomic potentials. Damage accumulation was modeled using a kinetic Monte Carlo (kMC) algorithm to follow the evolution of all defects produced in the cascades. The diffusivities and binding energies of defects are input data for this simulation and were either extracted from experiments, the literature, or calculated using MD. MD simulations reveal that vacancy clusters are produced within the cascade core in the case of copper. In iron, most of the vacancies do not cluster during cooling of the cascade core and are available for diffusion. In addition, self-interstitial atom (SIA) clusters are produced in copper cascades but those observed in iron are smaller in number and size. The combined MD/kMC simulations reveal that the visible cluster densities obtained as a function of dose are at least one order of magnitude lower in Fe than in Cu. We compare the results with experimental measurements of cluster density and find excellent agreement between the simulations and experiments when small interstitial clusters are considered to be mobile as suggested by recent MD simulations.

  4. Improving fatigue damage resistance of alumina through surface grading.

    PubMed

    Ren, L; Liu, L; Bhowmick, S; Gerbig, Y B; Janal, M N; Thompson, V P; Zhang, Y

    2011-08-01

    Porcelain-veneered alumina crown restorations often fail from bulk fracture resulting from radial cracks that initiate at the cementation surface with repeated flexure of the stiffer crown layers on the soft dentin support. We hypothesized that bulk fracture may be substantially mitigated by grading the elastic modulus at the crown surfaces. In this study, we fabricated graded structures by infiltrating glass into dense alumina plates, resulting in a diminished modulus at the surface layers. The plates were then bonded to polycarbonate substrates and subjected to fatigue loading in water. Tests were terminated when fracture occurred at the cementation tensile surface or at the fatigue endurance limit (1 million cycles). Infiltrated specimens showed a significant increase in fatigue fracture loads over non-infiltrated controls. Our results indicate that controlled elastic gradients at the surface could be highly beneficial in the design of fracture-resistant alumina crowns.

  5. Improving Fatigue Damage Resistance of Alumina through Surface Grading

    PubMed Central

    Ren, L.; Liu, L.; Bhowmick, S.; Gerbig, Y.B.; Janal, M.N.; Thompson, V.P.; Zhang, Y.

    2011-01-01

    Porcelain-veneered alumina crown restorations often fail from bulk fracture resulting from radial cracks that initiate at the cementation surface with repeated flexure of the stiffer crown layers on the soft dentin support. We hypothesized that bulk fracture may be substantially mitigated by grading the elastic modulus at the crown surfaces. In this study, we fabricated graded structures by infiltrating glass into dense alumina plates, resulting in a diminished modulus at the surface layers. The plates were then bonded to polycarbonate substrates and subjected to fatigue loading in water. Tests were terminated when fracture occurred at the cementation tensile surface or at the fatigue endurance limit (1 million cycles). Infiltrated specimens showed a significant increase in fatigue fracture loads over non-infiltrated controls. Our results indicate that controlled elastic gradients at the surface could be highly beneficial in the design of fracture-resistant alumina crowns. PMID:21555776

  6. Estimation of Fatigue Damage for an Austenitic Stainless Steel (SUS304) Using Magnetic Methods

    SciTech Connect

    Oka, M.; Yakushiji, T.; Tsuchida, Y.; Enokizono, M.

    2007-03-21

    There are some fatigue damage estimation methods of the austenitic stainless steel that uses the martensitic transformation. For instance, they are the remanent magnetization method, the excitation method, and so on. Those two methods are being researched also in our laboratory now. In the remanent magnetization method, it is well known that the relation between fatigue damage and the remanent magnetization is simple, clear, and reproducible. However, this method has the disadvantage to need a special magnetizer. This method cannot be easily used on the site such as the factory. On the other hand, because the special magnetizer is unnecessary, the excitation method can use easily on the site. The output signal of this method is small. In this paper, two fatigue evaluation methods such as the remanent magnetization method and the excitation method are introduced. In addition, we report on the result of comparing the fatigue evaluation performances of two methods.

  7. Estimation of Fatigue Damage for an Austenitic Stainless Steel (SUS304) Using Magnetic Methods

    NASA Astrophysics Data System (ADS)

    Oka, M.; Yakushiji, T.; Tsuchida, Y.; Enokizono, M.

    2007-03-01

    There are some fatigue damage estimation methods of the austenitic stainless steel that uses the martensitic transformation. For instance, they are the remanent magnetization method, the excitation method, and so on. Those two methods are being researched also in our laboratory now. In the remanent magnetization method, it is well known that the relation between fatigue damage and the remanent magnetization is simple, clear, and reproducible. However, this method has the disadvantage to need a special magnetizer. This method cannot be easily used on the site such as the factory. On the other hand, because the special magnetizer is unnecessary, the excitation method can use easily on the site. The output signal of this method is small. In this paper, two fatigue evaluation methods such as the remanent magnetization method and the excitation method are introduced. In addition, we report on the result of comparing the fatigue evaluation performances of two methods.

  8. Simplification of Fatigue Test Requirements for Damage Tolerance of Composite Interstage Launch Vehicle Hardware

    NASA Technical Reports Server (NTRS)

    Nettles, A. T.; Hodge, A. J.; Jackson, J. R.

    2010-01-01

    The issue of fatigue loading of structures composed of composite materials is considered in a requirements document that is currently in place for manned launch vehicles. By taking into account the short life of these parts, coupled with design considerations, it is demonstrated that the necessary coupon level fatigue data collapse to a static case. Data from a literature review of past studies that examined compressive fatigue loading after impact and data generated from this experimental study are presented to support this finding. Damage growth, in the form of infrared thermography, was difficult to detect due to rapid degradation of compressive properties once damage growth initiated. Unrealistically high fatigue amplitudes were needed to fail 5 of 15 specimens before 10,000 cycles were reached. Since a typical vehicle structure, such as the Ares I interstage, only experiences a few cycles near limit load, it is concluded that static compression after impact (CAI) strength data will suffice for most launch vehicle structures.

  9. An Intelligent Sensor System for Monitoring Fatigue Damage in Welded Steel Components

    SciTech Connect

    Fernandes, B.; Gaydecki, P.; Burdekin, F. Michael

    2005-04-09

    A system for monitoring fatigue damage in steel components is described. The sensor, a thin steel sheet with a pre-crack in it, is attached to the component. Its crack length increases by fatigue in service and is recorded using a microcontroller. Measurement is accomplished using conductive tracks in a circuit whose output voltage changes when the crack propagates past a track. Data stored in memory can be remotely downloaded using Bluetooth{sup TM} technology to a PC.

  10. An Intelligent Sensor System for Monitoring Fatigue Damage in Welded Steel Components

    NASA Astrophysics Data System (ADS)

    Fernandes, B.; Gaydecki, P.; Burdekin, F. Michael

    2005-04-01

    A system for monitoring fatigue damage in steel components is described. The sensor, a thin steel sheet with a pre-crack in it, is attached to the component. Its crack length increases by fatigue in service and is recorded using a microcontroller. Measurement is accomplished using conductive tracks in a circuit whose output voltage changes when the crack propagates past a track. Data stored in memory can be remotely downloaded using Bluetooth™ technology to a PC.

  11. Fatigue

    MedlinePlus

    ... to help you find out what's causing your fatigue and recommend ways to relieve it. Fatigue itself is not a disease. Medical problems, treatments, and personal habits can add to fatigue. These include Taking certain medicines, such as antidepressants, ...

  12. Analysis and prediction of Multiple-Site Damage (MSD) fatigue crack growth

    NASA Technical Reports Server (NTRS)

    Dawicke, D. S.; Newman, J. C., Jr.

    1992-01-01

    A technique was developed to calculate the stress intensity factor for multiple interacting cracks. The analysis was verified through comparison with accepted methods of calculating stress intensity factors. The technique was incorporated into a fatigue crack growth prediction model and used to predict the fatigue crack growth life for multiple-site damage (MSD). The analysis was verified through comparison with experiments conducted on uniaxially loaded flat panels with multiple cracks. Configuration with nearly equal and unequal crack distribution were examined. The fatigue crack growth predictions agreed within 20 percent of the experimental lives for all crack configurations considered.

  13. Fatigue damage evaluation of austenitic stainless steel using nonlinear ultrasonic waves in low cycle regime

    SciTech Connect

    Zhang, Jianfeng; Xuan, Fu-Zhen

    2014-05-28

    The interrupted low cycle fatigue test of austenitic stainless steel was conducted and the dislocation structure and fatigue damage was evaluated subsequently by using both transmission electron microscope and nonlinear ultrasonic wave techniques. A “mountain shape” correlation between the nonlinear acoustic parameter and the fatigue life fraction was achieved. This was ascribed to the generation and evolution of planar dislocation structure and nonplanar dislocation structure such as veins, walls, and cells. The “mountain shape” correlation was interpreted successfully by the combined contribution of dislocation monopole and dipole with an internal-stress dependent term of acoustic nonlinearity.

  14. Fatigue damage of notched boron/epoxy laminates under constant amplitude loading

    NASA Technical Reports Server (NTRS)

    Roderick, G. L.; Whitcomb, J. D.

    1976-01-01

    Fatigue damage in (0, + or - 45) and (0, + or - 45,90) boron/epoxy laminates was studied with X-ray radiography and scanning electron microscopy. In addition, limited tests for residual strength and stiffness were performed. The results of this study suggest that in boron/epoxy laminates the 45-degree plies play a key role in the fatigue process of boron/epoxy laminates that contain them. The fatigue process in the + or - 45-degree plies starts as intralaminar matrix cracks.

  15. Fatigue damage localization using time-domain features extracted from nonlinear Lamb waves

    NASA Astrophysics Data System (ADS)

    Hong, Ming; Su, Zhongqing; Lu, Ye; Cheng, Li

    2014-03-01

    Nonlinear guided waves are sensitive to small-scale fatigue damage that may hardly be identified by traditional techniques. A characterization method for fatigue damage is established based on nonlinear Lamb waves in conjunction with the use of a piezoelectric sensor network. Theories on nonlinear Lamb waves for damage detection are first introduced briefly. Then, the ineffectiveness of using pure frequency-domain information of nonlinear wave signals for locating damage is discussed. With a revisit to traditional gross-damage localization techniques based on the time of flight, the idea of using temporal signal features of nonlinear Lamb waves to locate fatigue damage is introduced. This process involves a time-frequency analysis that enables the damage-induced nonlinear signal features, which are either undiscernible in the original time history or uninformative in the frequency spectrum, to be revealed. Subsequently, a finite element modeling technique is employed, accounting for various sources of nonlinearities in a fatigued medium. A piezoelectric sensor network is configured to actively generate and acquire probing Lamb waves that involve damageinduced nonlinear features. A probability-based diagnostic imaging algorithm is further proposed, presenting results in diagnostic images intuitively. The approach is experimentally verified on a fatigue-damaged aluminum plate, showing reasonably good accuracy. Compared to existing nonlinear ultrasonics-based inspection techniques, this approach uses a permanently attached sensor network that well accommodates automated online health monitoring; more significantly, it utilizes time-domain information of higher-order harmonics from time-frequency analysis, and demonstrates a great potential for quantitative characterization of small-scale damage with improved localization accuracy.

  16. Thermo-elastic nondestructive evaluation of fatigue damage in PMR-15 resin

    NASA Astrophysics Data System (ADS)

    Welter, J. T.; Sathish, S.; Tandon, G. P.; Schehl, N.; Cherry, M.; Nalladega, V.; Lindgren, E. A.; Hall, R.

    2012-05-01

    Thermoset polyimide resins are used as the polymer matrix in high temperature composites for aerospace applications such as engine shrouds. At these locations the components have to withstand high temperatures and significant vibration. A number of studies have investigated the effects of thermal exposure on mechanical properties of polyimide resins, and the effects of fatigue on thermoplastics have been discussed at length. However, the effects of fatigue on thermosets, in particular polyimides, have largely been overlooked. In this paper we present studies of nondestructive evaluation of fatigue damage in a thermoset polyimide resin, PMR-15, performed by measuring the changes in the evolution of heat in the samples during cyclic loading. The temperature changes are measured using a high sensitivity IR camera as a function of number of fatigue cycles. Interrupted fatigue tests were performed on four samples. The temperature rise during an increment of fatigue cycling shows two linear regions each with a different slope (region 1 and region 2). Region 1 remains constant for every increment of fatigue, while region 2 increases. The onset of region 2 occurs at the same increase in temperature due to hysteretic heating for all samples. Experimental observations are explained using a phenomenological two phase model based on crosslinking density variations in observed in other thermoset resins at microscopic scales. The results of these experiments are discussed in reference to utilizing this technique for detection and evaluation of fatigue in PMR-15 resin and composites.

  17. Damage-based life prediction model for uniaxial low-cycle stress fatigue of super-elastic NiTi shape memory alloy microtubes

    NASA Astrophysics Data System (ADS)

    Song, Di; Kang, Guozheng; Kan, Qianhua; Yu, Chao; Zhang, Chuanzeng

    2015-08-01

    Based on the experimental observations for the uniaxial low-cycle stress fatigue failure of super-elastic NiTi shape memory alloy microtubes (Song et al 2015 Smart Mater. Struct. 24 075004) and a new definition of damage variable corresponding to the variation of accumulated dissipation energy, a phenomenological damage model is proposed to describe the damage evolution of the NiTi microtubes during cyclic loading. Then, with a failure criterion of Dc = 1, the fatigue lives of the NiTi microtubes are predicted by the damage-based model, the predicted lives are in good agreement with the experimental ones, and all of the points are located within an error band of 1.5 times.

  18. An investigation on low frequency fatigue damage of mooring lines applied in a semi-submersible platform

    NASA Astrophysics Data System (ADS)

    Du, Junfeng; Wang, Shuqing; Chang, Anteng; Li, Huajun

    2016-06-01

    Assessing the fatigue life of mooring systems is important for deep water structures. In this paper, a comprehensive fatigue analysis is conducted on the mooring lines applied in a semi-submersible platform with special focus on the low frequency (LF) fatigue damage. Several influential factors, including water depth, wave spectral parameters, and riser system, are considered. Numerical simulation of a semi-submersible platform with the mooring/riser system is executed under different conditions, and the fatigue damage of mooring lines is assessed by using the time domain analysis method as a benchmark. The effects of these factors on the mooring line tension and the fatigue damage are investigated and discussed in detail. Research results indicate that the LF fatigue damage only accounts for a very small portion of the total damage, although the LF components dominate the global motion response and the mooring line tension of the semi-submersible platform. However, it is demonstrated that the LF fatigue damage is clearly affected by the influential factors. The increase in water depth and spectral peak periods, and the existence of risers can weaken the contribution of the LF components to the mooring line fatigue damage, while the fatigue damage due to the LF components increases with the increase of significant wave height.

  19. Evaluation of Creep-Fatigue Damage Based on Simplified Model Test Approach

    SciTech Connect

    Wang, Yanli; Li, Tianlei; Sham, Sam; Jetter, Robert I

    2013-01-01

    Current methods used in the ASME Code, Subsection NH for the evaluation of creep-fatigue damage are based on the separation of elevated temperature cyclic damage into two parts, creep damage and fatigue damage. This presents difficulties in both evaluation of test data and determination of cyclic damage in design. To avoid these difficulties, an alternative approach was identified, called the Simplified Model Test or SMT approach based on the use of creep-fatigue hold time test data from test specimens with elastic follow-up conservatively designed to bound the response of general structural components of interest. A key feature of the methodology is the use of the results of elastic analysis directly in design evaluation similar to current methods in the ASME Code, Subsection NB. Although originally developed for current material included in Subsection NH, recent interest in the application of Alloy 617 for components operating at very high temperatures has caused renewed interest in the SMT approach because it provides an alternative to the proposed restriction on the use of current Subsection NH simplified methods at very high temperatures. A comprehensive review and assessment of five representative simplified methods for creep-fatigue damage evaluation is presented in Asayama [1]. In this review the SMT methodology was identified as the best long term approach but the need for test data precluded its near term implementation. Asayama and Jetter [2] is a summary of the more comprehensive report by Asayama [1] with a summary of the SMT approach presented by Jetter [3].

  20. The application of the load-stroke hysteresis technique for evaluating fatigue damage development

    SciTech Connect

    Baxter, T.; Reifsnider, K.L.

    1994-12-31

    A new experimental method was developed to measure hysteresis loss during a fatigue test from the load and stroke signals of a standard servo-hydraulic materials testing system. The method was used to characterize changes in properties and performance induced by long-term cyclic loading. Advantages of the load-stroke hysteresis measurement include: (1) contact with the specimen is not required, (2) the fatigue test is not interrupted for data collection, (3) the measured quantity (the hysteresis loop area) is directly related to the (damage) events that alter material properties and life, and (4) a quantitative measure of damage extent and development rate is obtained. The method was used to evaluate damage development during fatigue tests of polymeric composite laminates with unidirectional and angle-ply fiber orientations. The hysteresis loop measurements were used to identify the different stages of damage development and the different damage mechanisms (matrix cracking, delamination, and fiber fracture) in the material systems. The results from the hysteresis technique were correlated with conventional NDE methods such as dynamic signal analysis and specimen surface temperature measurements. It was found that the load-stroke hysteresis technique was especially sensitive to the fiber fracture, the most difficult type of damage process to interrogate in-situ. The hysteresis technique may provide a valuable method for predicting fatigue failure in composite specimens.

  1. Fatigue damage assessment in 7075 and 7050 aluminum alloys at low cyclic stresses

    NASA Astrophysics Data System (ADS)

    Malast, Mary Kathryn

    Dynamic loads from buffeting, shock waves, and separated flow cause aircraft structural fatigue. Increases in aircraft performance cause sonic high cycle fatigue (HCF) in structural components. The accuracy of HCF damage predictions and fracture mechanics analysis has been limited in the past by the maximum cyclic rate of fatigue test equipment. Constant load amplitude axial fatigue data has previously been acquired at rates on the order of 100 Hz or less. Understanding HCF damage mechanisms has been hindered by this limitation. Recent improvements in test technology and analysis methods have made axial HCF experiments practical. X-ray diffraction line broadening analysis is used here to quantify microstructural changes in 7075-T651 and 7050-T7451 precipitation hardened aluminum alloys after exposure to fatigue loading. The Stokes deconvolution and Warren-Averbach method are used to compute size and strain broadening from line broadened X-ray diffraction patterns. Changes in domain size, strain, domain size anisotropy, and particle size distribution are used to assess fatigue in the alloys. Mechanisms which account for the observed microstructural changes are proposed. Peak splitting produces double maxima in the X-ray diffraction data. Double maxima are previously reported in the literature. Possible sources of the double maxima in precipitation hardened aluminum alloys are considered. In addition to assessing fatigue related microstructural changes in 7075 and 7050 aluminum alloys, the line broadening method applied to fatigue related assessment of these alloys is determined to be a promising approach. The method may have application to evaluation of aircraft in production. Possible applications and future work needed to apply the line broadening method to damage evaluation of engineering problems are proposed.

  2. Fatigue crack damage detection using subharmonic component with nonlinear boundary condition

    SciTech Connect

    Wu, Weiliang Qu, Wenzhong E-mail: xiaoli6401@126.com; Xiao, Li E-mail: xiaoli6401@126.com; Shen, Yanfeng Giurgiutiu, Victor

    2015-03-31

    In recent years, researchers have focused on structural health monitoring (SHM) and damage detection techniques using nonlinear vibration and nonlinear ultrasonic methods. Fatigue cracks may exhibit contact acoustic nonlinearity (CAN) with distinctive features such as superharmonics and subharmonics in the power spectrum of the sensing signals. However, challenges have been noticed in the practical applications of the harmonic methods. For instance, superharmonics can also be generated by the piezoelectric transducers and the electronic equipment; super/subharmonics may also stem from the nonlinear boundary conditions such as structural fixtures and joints. It is hard to tell whether the nonlinear features come from the structural damage or the intrinsic nonlinear boundary conditions. The objective of this paper is to demonstrate the application of nonlinear ultrasonic subharmonic method for detecting fatigue cracks with nonlinear boundary conditions. The fatigue crack was qualitatively modeled as a single-degree-of-freedom (SDOF) system with non-classical hysteretic nonlinear interface forces at both sides of the crack surfaces. The threshold of subharmonic generation was studied, and the influence of crack interface parameters on the subharmonic resonance condition was investigated. The different threshold behaviors between the nonlinear boundary condition and the fatigue crack was found, which can be used to distinguish the source of nonlinear subharmonic features. To evaluate the proposed method, experiments of an aluminum plate with a fatigue crack were conducted to quantitatively verify the subharmonic resonance range. Two surface-bonded piezoelectric transducers were used to generate and receive ultrasonic wave signals. The fatigue damage was characterized in terms of a subharmonic damage index. The experimental results demonstrated that the subharmonic component of the sensing signal can be used to detect the fatigue crack and further distinguish it from

  3. Fatigue crack damage detection using subharmonic component with nonlinear boundary condition

    NASA Astrophysics Data System (ADS)

    Wu, Weiliang; Shen, Yanfeng; Qu, Wenzhong; Xiao, Li; Giurgiutiu, Victor

    2015-03-01

    In recent years, researchers have focused on structural health monitoring (SHM) and damage detection techniques using nonlinear vibration and nonlinear ultrasonic methods. Fatigue cracks may exhibit contact acoustic nonlinearity (CAN) with distinctive features such as superharmonics and subharmonics in the power spectrum of the sensing signals. However, challenges have been noticed in the practical applications of the harmonic methods. For instance, superharmonics can also be generated by the piezoelectric transducers and the electronic equipment; super/subharmonics may also stem from the nonlinear boundary conditions such as structural fixtures and joints. It is hard to tell whether the nonlinear features come from the structural damage or the intrinsic nonlinear boundary conditions. The objective of this paper is to demonstrate the application of nonlinear ultrasonic subharmonic method for detecting fatigue cracks with nonlinear boundary conditions. The fatigue crack was qualitatively modeled as a single-degree-of-freedom (SDOF) system with non-classical hysteretic nonlinear interface forces at both sides of the crack surfaces. The threshold of subharmonic generation was studied, and the influence of crack interface parameters on the subharmonic resonance condition was investigated. The different threshold behaviors between the nonlinear boundary condition and the fatigue crack was found, which can be used to distinguish the source of nonlinear subharmonic features. To evaluate the proposed method, experiments of an aluminum plate with a fatigue crack were conducted to quantitatively verify the subharmonic resonance range. Two surface-bonded piezoelectric transducers were used to generate and receive ultrasonic wave signals. The fatigue damage was characterized in terms of a subharmonic damage index. The experimental results demonstrated that the subharmonic component of the sensing signal can be used to detect the fatigue crack and further distinguish it from

  4. Observations of fatigue crack initiation and damage growth in notched titanium matrix composites

    NASA Technical Reports Server (NTRS)

    Naik, R. A.; Johnson, W. S.

    1990-01-01

    The purpose was to characterize damage initiation and growth in notched titanium matrix composites at room temperature. Double edge notched or center open hole SCS-6/Ti-15-3 specimens containing 0 deg plies or containing both 0 and 90 deg plies were fatigued. The specimens were tested in the as-fabricated (ASF) and in heat-treated conditions. A local strain criterion using unnotched specimen fatigue data was successful in predicting fatigue damage initiation. The initiation stress level was accurately predicted for both a double edge notched unidirectional specimen and a cross-plied center hole specimen. The fatigue produced long multiple cracks growing from the notches. These fatigue cracks were only in the matrix material and did not break the fibers in their path. The combination of matrix cracking and fiber/matrix debonding appears to greatly reduce the stress concentration around the notches. The laminates that were heat treated showed a different crack growth pattern. In the ASF specimens, matrix cracks had a more tortuous path and showed considerable more crack branching. For the same specimen geometry and cyclic stress, the (0/90/0) laminate with a hole had far superior fatigue resistance than the matrix only specimen with a hole.

  5. Modeling nonlinearities of ultrasonic waves for fatigue damage characterization: theory, simulation, and experimental validation.

    PubMed

    Hong, Ming; Su, Zhongqing; Wang, Qiang; Cheng, Li; Qing, Xinlin

    2014-03-01

    A dedicated modeling technique for comprehending nonlinear characteristics of ultrasonic waves traversing in a fatigued medium was developed, based on a retrofitted constitutive relation of the medium by considering the nonlinearities originated from material, fatigue damage, as well as the "breathing" motion of fatigue cracks. Piezoelectric wafers, for exciting and acquiring ultrasonic waves, were integrated in the model. The extracted nonlinearities were calibrated by virtue of an acoustic nonlinearity parameter. The modeling technique was validated experimentally, and the results showed satisfactory consistency in between, both revealing: the developed modeling approach is able to faithfully simulate fatigue crack-incurred nonlinearities manifested in ultrasonic waves; a cumulative growth of the acoustic nonlinearity parameter with increasing wave propagation distance exists; such a parameter acquired via a sensing path is nonlinearly related to the offset distance from the fatigue crack to that sensing path; and neither the incidence angle of the probing wave nor the length of the sensing path impacts on the parameter significantly. This study has yielded a quantitative characterization strategy for fatigue cracks using embeddable piezoelectric sensor networks, facilitating deployment of structural health monitoring which is capable of identifying small-scale damage at an embryo stage and surveilling its growth continuously.

  6. Experimental and analytical study of fatigue damage in notched graphite/epoxy laminates

    NASA Technical Reports Server (NTRS)

    Whitcomb, J. D.

    1979-01-01

    Both tension and compression fatigue behaviors were investigated in four notched graphite/epoxy laminates. After fatigue loading, specimens were examined for damage type and location using visual inspection, light microscopy, scanning electron microscopy, ultrasonic C-scans, and X-radiography. Delamination and ply cracking were found to be the dominant types of fatigue damage. In general, ply cracks did not propagate into adjacent plies of differing fiber orientation. To help understand the varied fatigue observations, the interlaminar stress distribution was calculated with finite element analysis for the regions around the hole and along the straight free edge. Comparison of observed delamination locations with the calculated stresses indicated that both interlaminar shear and peel stresses must be considered when predicting delamination. The effects of the fatigue cycling on residual strength and stiffness were measured for some specimens of each laminate type. Fatigue loading generally caused only small stiffness losses. In all cases, residual strengths were greater than or equal to the virgin strengths.

  7. Fatigue analysis of multiple site damage at a row of holes in a wide panel

    NASA Technical Reports Server (NTRS)

    Buhler, Kimberley; Grandt, Alten F., Jr.; Moukawsher, E. J.

    1994-01-01

    This paper is concerned with predicting the fatigue life of unstiffened panels which contain multiple site damage (MSD). The initial damage consists of through-the-thickness cracks emanating from a row of holes in the center of a finite width panel. A fracture mechanics analysis has been developed to predict the growth, interaction, and coalescence of the various cracks which propagate in the panel. A strain-life analysis incorporating Neuber's rule for notches, and Miner's rule for cumulative damage, is also employed to predict crack initiation for holes without initial cracking. This analysis is compared with the results of a series of fatigue tests on 2024-T3 aluminum panels, and is shown to do an excellent job of predicting the influence of MSD on the fatigue life of nine inch wide specimens. Having established confidence in the ability to analyze the influence of MSD on fatigue life, a parametric study is conducted to examine the influence of various MSD scenarios in an unstiffened panel. The numerical study considered 135 cases in all, with the parametric variables being the applied cyclic stress level, the lead crack geometry, and the number and location of MSD cracks. The numerical analysis provides details for the manner in which lead cracks and MSD cracks grow and coalesce leading to final failure. The results indicate that MSD located adjacent to lead cracks is the most damaging configuration, while for cases without lead cracks, MSD clusters which are not separated by uncracked holes are most damaging.

  8. Cyclic fatigue damage characteristics observed for simple loadings extended to multiaxial life prediction

    NASA Technical Reports Server (NTRS)

    Jones, David J.; Kurath, Peter

    1988-01-01

    Fully reversed uniaxial strain controlled fatigue tests were performed on smooth cylindrical specimens made of 304 stainless steel. Fatigue life data and cracking observations for uniaxial tests were compared with life data and cracking behavior observed in fully reversed torsional tests. It was determined that the product of maximum principle strain amplitude and maximum principle stress provided the best correlation of fatigue lives for these two loading conditions. Implementation of this parameter is in agreement with observed physical damage and it accounts for the variation of stress-strain response, which is unique to specific loading conditions. Biaxial fatigue tests were conducted on tubular specimens employing both in-phase and out-of-phase tension torsion cyclic strain paths. Cracking observations indicated that the physical damage which occurred in the biaxial tests was similar to the damage observed in uniaxial and torsional tests. The Smith, Watson, and Topper parameter was then extended to predict the fatigue lives resulting from the more complex loading conditions.

  9. Thermography Inspection for Early Detection of Composite Damage in Structures During Fatigue Loading

    NASA Technical Reports Server (NTRS)

    Zalameda, Joseph N.; Burke, Eric R.; Parker, F. Raymond; Seebo, Jeffrey P.; Wright, Christopher W.; Bly, James B.

    2012-01-01

    Advanced composite structures are commonly tested under controlled loading. Understanding the initiation and progression of composite damage under load is critical for validating design concepts and structural analysis tools. Thermal nondestructive evaluation (NDE) is used to detect and characterize damage in composite structures during fatigue loading. A difference image processing algorithm is demonstrated to enhance damage detection and characterization by removing thermal variations not associated with defects. In addition, a one-dimensional multilayered thermal model is used to characterize damage. Lastly, the thermography results are compared to other inspections such as non-immersion ultrasonic inspections and computed tomography X-ray.

  10. The Characteristics of Fatigue Damage in the Fuselage Riveted Lap Splice Joint

    NASA Technical Reports Server (NTRS)

    Piascik, Robert S.; Willard, Scott A.

    1997-01-01

    An extensive data base has been developed to form the physical basis for new analytical methodology to predict the onset of widespread fatigue damage in the fuselage lap splice joint. The results of detailed destructive examinations have been cataloged to describe the physical nature of MSD in the lap splice joint. ne catalog includes a detailed description, e.g., crack initiation, growth rates, size, location, and fracture morphology, of fatigue damage in the fuselage lap splice joint structure. Detailed examinations were conducted on a lap splice joint panel removed from a full scale fuselage test article after completing a 60,000 cycle pressure test. The panel contained a four bay region that exhibited visible outer skin cracks and regions of crack link-up along the upper rivet row. Destructive examinations revealed undetected fatigue damage in the outer skin, inner skin, and tear strap regions. Outer skin fatigue cracks were found to initiate by fretting damage along the faying surface. The cracks grew along the faying surface to a length equivalent to two to three skin thicknesses before penetrating the outboard surface of the outer skin. Analysis of fracture surface marker bands produced during full scale testing revealed that all upper rivet row fatigue cracks contained in a dim bay region grow at similar rates; this important result suggests that fracture mechanics based methods can be used to predict the growth of outer skin fatigue cracks in lap splice structure. Results are presented showing the affects of MSD and out-of-plane pressure loads on outer skin crack link-up.

  11. 14 CFR 23.573 - Damage tolerance and fatigue evaluation of structure.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Damage tolerance and fatigue evaluation of structure. 23.573 Section 23.573 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF... operational life of the airplane must be consistent with the initial detectability and subsequent growth...

  12. Death rates reflect accumulating brain damage in arthropods

    PubMed Central

    Fonseca, Duane B; Brancato, Carolina L; Prior, Andrew E; Shelton, Peter M.J; Sheehy, Matt R.J

    2005-01-01

    We present the results of the first quantitative, whole-lifespan study of the relationship between age-specific neurolipofuscin concentration and natural mortality rate in any organism. In a convenient laboratory animal, the African migratory locust, Locusta migratoria, we find an unusual delayed-onset neurolipofuscin accumulation pattern that is highly correlated with exponentially accelerating age-specific Gompertz–Makeham death rates in both males (r=0.93, p=0.0064) and females (r=0.97, p=0.0052). We then test the conservation of this association by aggregating the locust results with available population-specific data for a range of other terrestrial, freshwater, marine, tropical and temperate arthropods whose longevities span three orders of magnitude. This synthesis shows that the strong association between neurolipofuscin deposition and natural mortality is a phylogenetically and environmentally widespread phenomenon (r=0.96, p<0.0001). These results highlight neurolipofuscin as a unique and outstanding integral biomarker of ageing. They also offer compelling evidence for the proposal that, in vital organs like the brain, either the accumulation of toxic garbage in the form of lipofuscin itself, or the particular molecular reactions underlying lipofuscinogenesis, including free-radical damage, are the primary events in senescence. PMID:16191601

  13. Death rates reflect accumulating brain damage in arthropods.

    PubMed

    Fonseca, Duane B; Brancato, Carolina L; Prior, Andrew E; Shelton, Peter M J; Sheehy, Matt R J

    2005-09-22

    We present the results of the first quantitative, whole-lifespan study of the relationship between age-specific neurolipofuscin concentration and natural mortality rate in any organism. In a convenient laboratory animal, the African migratory locust, Locusta migratoria, we find an unusual delayed-onset neurolipofuscin accumulation pattern that is highly correlated with exponentially accelerating age-specific Gompertz-Makeham death rates in both males (r=0.93, p=0.0064) and females (r=0.97, p=0.0052). We then test the conservation of this association by aggregating the locust results with available population-specific data for a range of other terrestrial, freshwater, marine, tropical and temperate arthropods whose longevities span three orders of magnitude. This synthesis shows that the strong association between neurolipofuscin deposition and natural mortality is a phylogenetically and environmentally widespread phenomenon (r=0.96, p < 0.0001). These results highlight neurolipofuscin as a unique and outstanding integral biomarker of ageing. They also offer compelling evidence for the proposal that, in vital organs like the brain, either the accumulation of toxic garbage in the form of lipofuscin itself, or the particular molecular reactions underlying lipofuscinogenesis, including free-radical damage, are the primary events in senescence.

  14. Locating fatigue damage using temporal signal features of nonlinear Lamb waves

    NASA Astrophysics Data System (ADS)

    Hong, Ming; Su, Zhongqing; Lu, Ye; Sohn, Hoon; Qing, Xinlin

    2015-08-01

    The temporal signal features of linear guided waves, as typified by the time-of-flight (ToF), have been exploited intensively for identifying damage, with proven effectiveness in locating gross damage in particular. Upon re-visiting the conventional, ToF-based detection philosophy, the present study extends the use of temporal signal processing to the realm of nonlinear Lamb waves, so as to reap the high sensitivity of nonlinear Lamb waves to small-scale damage (e.g., fatigue cracks), and the efficacy of temporal signal processing in locating damage. Nonlinear wave features (i.e., higher-order harmonics) are extracted using networked, miniaturized piezoelectric wafers, and reverted to the time domain for damage localization. The proposed approach circumvents the deficiencies of using Lamb wave features for evaluating undersized damage, which are either undiscernible in time-series analysis or lacking in temporal information in spectral analysis. A probabilistic imaging algorithm is introduced to supplement the approach, facilitating the presentation of identification results in an intuitive manner. Through numerical simulation and then experimental validation, two damage indices (DIs) are comparatively constructed, based, respectively, on linear and nonlinear temporal features of Lamb waves, and used to locate fatigue damage near a rivet hole of an aluminum plate. Results corroborate the feasibility and effectiveness of using temporal signal features of nonlinear Lamb waves to locate small-scale fatigue damage, with enhanced accuracy compared with linear ToF-based detection. Taking a step further, a synthesized detection strategy is formulated by amalgamating the two DIs, targeting continuous and adaptive monitoring of damage from its onset to macroscopic formation.

  15. Non-Destructive Evaluation of Fatigue Damage for SUS316 by Using Electromagnetic Methods

    NASA Astrophysics Data System (ADS)

    Oka, M.; Tsuchida, Y.; Yakushiji, T.; Enokizono, M.

    2009-03-01

    There are some fatigue damage estimation methods for an austenitic stainless steel that uses martensitic transformation. For instance, those are the remanent magnetization method, the excitation method using the differential pick-up coil, and so on. We are researching also those two methods in our laboratory now. In the remanent magnetization method, it is well known that the relationship between fatigue damage and the remanent magnetization is simple, clear, and reproducible. In addition, the excitation method can be easily used at the job site because the special magnetizer is unnecessary. But, these methods have some disadvantages shown as follows. For instance, the former needs a special magnetizer and the latter's output signal is small. On the other hand, it is well known that the inductance of a pancake type coil put on the metallic specimen changes according to the electromagnetic properties of the metallic specimen. In this paper, the assessment method of fatigue of an austenitic stainless steel (SUS316) that uses the change by fatigue of the inductance of the pancake type coil measured with the LCR meter is shown. In addition, the fatigue evaluation performance of this method is described.

  16. On the Effect of Ramp Rate in Damage Accumulation of the CPV Die-Attach: Preprint

    SciTech Connect

    Bosco, N. S.; Silverman, T. J.; Kurtz, S. R.

    2012-06-01

    It is commonly understood that thermal cycling at high temperature ramp rates may activate unrepresentative failure mechanisms. Increasing the temperature ramp rate of thermal cycling, however, could dramatically reduce the test time required to achieve an equivalent amount of thermal fatigue damage, thereby reducing overall test time. Therefore, the effect of temperature ramp rate on physical damage in the CPV die-attach is investigated. Finite Element Model (FEM) simulations of thermal fatigue and thermal cycling experiments are made to determine if the amount of damage calculated results in a corresponding amount of physical damage measured to the die-attach for a variety of fast temperature ramp rates. Preliminary experimental results are in good agreement with simulations and reinforce the potential of increasing temperature ramp rates. Characterization of the microstructure and resulting fatigue crack in the die-attach suggest a similar failure mechanism across all ramp rates tested.

  17. A Coupled/Uncoupled Computational Scheme for Deformation and Fatigue Damage Analysis of Unidirectional Metal-Matrix Composites

    NASA Technical Reports Server (NTRS)

    Wilt, Thomas E.; Arnold, Steven M.; Saleeb, Atef F.

    1997-01-01

    A fatigue damage computational algorithm utilizing a multiaxial, isothermal, continuum-based fatigue damage model for unidirectional metal-matrix composites has been implemented into the commercial finite element code MARC using MARC user subroutines. Damage is introduced into the finite element solution through the concept of effective stress that fully couples the fatigue damage calculations with the finite element deformation solution. Two applications using the fatigue damage algorithm are presented. First, an axisymmetric stress analysis of a circumferentially reinforced ring, wherein both the matrix cladding and the composite core were assumed to behave elastic-perfectly plastic. Second, a micromechanics analysis of a fiber/matrix unit cell using both the finite element method and the generalized method of cells (GMC). Results are presented in the form of S-N curves and damage distribution plots.

  18. Dependence of the Perpendicular Residual Leakage Magnetic Flux Density on Fatigue Damage in an Austenitic Stainless Steel

    NASA Astrophysics Data System (ADS)

    Oka, M.; Yakushiji, T.; Tsuchida, Y.; Enokizono, M.

    2003-03-01

    In order to estimate the amount of plane bending fatigue damage in an austenitic stainless steel (SUS304), we were investigating the relationship between plane bending fatigue damage and the perpendicular residual leakage magnetic flux density caused by martensitic structure induced by plane bending fatigue. A specimen such as SUS304 had been excited in a constant external magnetic field perpendicularly to measure dependence of the perpendicular residual leakage magnetic flux density on plane bending fatigue damage accurately. The Z component of the magnetic flux density at 1 mm above a specimen is measured by using a thin-film flux-gate (FG) magnetic sensor. Residual magnetization is caused by partial martensitic structure in an austenitic stainless steel induced by cyclic bending stress. From our experiments, we can evaluate dependence of the perpendicular residual leakage magnetic flux density on plane bending fatigue damage and know the relationship between growth of a crack and the perpendicular residual leakage magnetic flux density.

  19. Experimental Evaluation of Fatigue Damage Progression in Postbuckled Single Stringer Composite Specimens

    NASA Technical Reports Server (NTRS)

    Bisagni, Chiara; Davila, Carlos G.; Rose, Cheryl A.; Zalameda, Joseph N.

    2014-01-01

    The durability and damage tolerance of postbuckled composite structures are not yet completely understood, and remain difficult to predict due to the nonlinearity of the geometric response and its interaction with local damage modes. A research effort was conducted to investigate experimentally the quasi-static and fatigue damage progression in a single-stringer compression (SSC) specimen. Three specimens were manufactured with a hat-stiffener, and an initial defect was introduced with a Teflon film embedded between one flange of the stringer and the skin. One of the specimens was tested under quasi-static compressive loading, while the remaining two specimens were tested by cycling in postbuckling. The tests were performed at the NASA Langley Research Center under controlled conditions and with instrumentation that allows a precise evaluation of the postbuckling response and of the damage modes. Three-dimensional digital image correlation VIC-3D systems were used to provide full field displacements and strains on the skin and the stringer. Passive thermal monitoring was conducted during the fatigue tests using an infrared camera that showed the location of the delamination front while the specimen was being cycled. The live information from the thermography was used to stop the fatigue tests at critical stages of the damage evolution to allow detailed ultrasonic scans.

  20. Influence of interface ply orientation on fatigue damage of adhesively bonded composite joints

    NASA Technical Reports Server (NTRS)

    Johnson, W. S.; Mall, S.

    1985-01-01

    An experimental study of cracked-lap-shear specimens was conducted to determine the influence of adherend stacking sequence on debond initiation and damage growth in a composite-to-composite bonded joint. Specimens consisted of quasi-isotropic graphite/epoxy adherends bonded together with either FM-300 or EC 3445 adhesives. The stacking sequence of the adherends was varied such that 0 deg, 45 deg, or 90 deg plies were present at the adherend-adhesive interfaces. Fatigue damage initiated in the adhesive layer in those specimens with 0 deg nd 45 deg interface plies. Damage initiated in the form of ply cracking in the strap adherend for the specimens with 90 deg interface plies. The fatigue-damage growth was in the form of delamination within the composite adherends for specimens with the 90 deg and 45 deg plies next to the adhesive, while debonding in the adhesive resulted for the specimens with 0 deg plies next to the adhesive. Those joints with the 0 deg and 45 deg plies next to either adhesive has essentially the same fatigue-damage-initiation stress levels. These stress levels were 13 and 71 percent higher, respectively, than those for specimens with 90 deg plies next to the EC 3445 and FM-300 adhesives.

  1. Real Time Fatigue Damage Growth Assessment of a Composite Three-Stringer Panel Using Passive Thermography

    NASA Technical Reports Server (NTRS)

    Zalameda, Joseph N.; Burke, Eric R.; Horne, Michael R.; Bly, James B.

    2015-01-01

    Fatigue testing of advanced composite structures is critical to validate both structural designs and damage prediction models. In-situ inspection methods are necessary to track damage onset and growth as a function of load cycles. Passive thermography is a large area, noncontact inspection technique that is used to detect composite damage onset and growth in real time as a function of fatigue cycles. The thermal images are acquired in synchronicity to the applied compressive load using a dual infrared camera acquisition system for full (front and back) coverage. Image processing algorithms are investigated to increase defect contrast areas. The thermal results are compared to non-immersion ultrasound inspections and acoustic emission data.

  2. Estimation of Fatigue Damage for AN Austenitic Stainless Steel (SUS304) Using a Pancake Type Coil

    NASA Astrophysics Data System (ADS)

    Oka, M.; Tsuchida, Y.; Nagato, S.; Yakushiji, T.; Enokizono, M.

    2008-02-01

    There are some fatigue damage estimation methods of an austenitic stainless steel that uses martensitic transformation. For instance, those are the remanent magnetization method, the excitation method, and so on. Those two methods are researched also in our laboratory now. In the remanent magnetization method, it is well known that the relationship between fatigue damage and the remanent magnetization is simple, clear, and reproducible. However, this method has the disadvantage to need a special magnetizer. Then, this method cannot be easily used at the job site such as the factory. On the other hand, as the special magnetizer is unnecessary, the excitation method can be easily used at the job site. But, this method has some disadvantages shown as follows. For instance, the output signal of this method is small. And the surface state of the specimen strongly influences the noise component of the output signal. It is well known that the inductance of a pancake type coil put on the metallic specimen changes according to the electromagnetic properties of the metallic specimen. In this paper, the method of evaluation of fatigue damage of an austenitic stainless steel (SUS304) by using a change of an inductance of a pancake type coil is shown. In addition, the fatigue evaluation performance of this method is described.

  3. Unlimited Damage Accumulation in Metallic Materials Under Cascade-Damage Conditions

    SciTech Connect

    Barashev, Aleksandr; Golubov, Stanislav I

    2008-09-01

    Most experiments on neutron or heavy-ion cascade-produced irradiation of pure metals and metallic alloys demonstrate unlimited void growth as well as development of the dislocation structure. In contrast, the theory of radiation damage predicts saturation of void swelling at sufficiently high irradiation doses and, accordingly, termination of accumulation of interstitial-type defects. It is shown in the present paper that, under conditions of steady production of one-dimensionally (1-D) mobile clusters of self-interstitial atoms (SIAs) in displacement cascades, any one of the following three conditions can result in indefinite damage accumulation. First, if the fraction of SIAs generated in the clustered form is smaller than some finite value of the order of the dislocation bias factor. Second, if solute, impurity or transmuted atoms form atmospheres around voids and repel the SIA clusters. Third, if spatial correlations between voids and other defects, such as second-phase precipitates and dislocations, exist that provide shadowing of voids from the SIA clusters. The driving force for the development of such correlations is the same as for void lattice formation and is argued to be always present under cascade-damage conditions. It is emphasised that the mean-free path of 1-D migrating SIA clusters is typically at least an order of magnitude longer than the average distance between microstructural defects; hence spatial correlations on the same scale should be taken into consideration. A way of developing a predictive theory is discussed. An interpretation

  4. Fatigue damage simulation of a laminated composite plate with a central hole

    NASA Astrophysics Data System (ADS)

    Lessard, Larry B.; Liu, Bangyan

    A FEM technique is here used in conjunction with a modulus-degradation model to simulate the progression of damage in a laminated composite plate, which has a central hole and is subjected to tension-tension fatigue loading, as a function of load level and number of load cycles. Analytical models are developed to predict the residual elastic moduli changes that are due to both matrix cracking and delamination. The scheme as a whole is sufficiently general for application to numerous additional problems involving fatigue-loaded composites with stress concentrations.

  5. Nondestructive indication of fatigue damage and residual lifetime in ferromagnetic construction materials

    NASA Astrophysics Data System (ADS)

    Tomáš, Ivan; Kovářík, Ondřej; Vértesy, Gábor; Kadlecová, Jana

    2014-06-01

    A new revolutionary attitude toward investigation of fatigue damage in cyclically loaded steel samples is reported. The measurement is based on the method of magnetic adaptive testing, which--in contrast to traditional magnetic hysteresis investigations--picks up the relevant information from systematic measurement and evaluation of whole minor magnetic hysteresis loops and their derivatives. Satisfactory correlations between nondestructively measured magnetic descriptors and actual lifetime of the fatigued material were found. The presented method is able to serve as a powerful tool for indication of changes, which occur in the structure of the inspected objects during their industrial service lifetime, as long as they are manufactured from ferromagnetic materials.

  6. Post-Impact Fatigue Damage Monitoring Using Fiber Bragg Grating Sensors

    PubMed Central

    Shin, Chow-Shing; Liaw, Shien-Kuei; Yang, Shi-Wei

    2014-01-01

    It has been shown that impact damage to composite materials can be revealed by embedded Fiber Bragg Gratings (FBG) as a broadening and splitting of the latter's characteristic narrow peak reflected spectrum. The current work further subjected the impact damaged composite to cyclic loading and found that the FBG spectrum gradually submerged into a rise of background intensity as internal damages progressed. By skipping the impact, directing the impact to positions away from the FBG and examining the extracted fibers, we concluded that the above change is not a result of deterioration/damage of the sensor. It is caused solely by the damages initiated in the composite by the impact and aggravated by fatigue loading. Evolution of the grating spectrum may therefore be used to monitor qualitatively the development of the incurred damages. PMID:24594609

  7. Fatigue damage modeling for coated single crystal superalloys

    NASA Technical Reports Server (NTRS)

    Nissley, David M.

    1988-01-01

    A high temperature, low-cycle fatigue life prediction method for coated single crystal nickel-base superalloys is being developed. The method is being developed for use in predicting crack initiation life of coated single crystal turbine airfoils. Although the models are being developed using coated single crystal PWA 1480, they should be readily adaptable to other coated nickel-base single crystal materials. The coatings choosen for this effort were of two generic types: a low pressure plasma sprayed NiCoCrAlY overlay, designated PWA 286, and an aluminide diffusion, designated PWA 273. In order to predict the useful crack initiation life of airfoils, the constitutive and failure behavior of the coating/substrate combination must be taken into account. Coatings alter the airfoil surface microstructure and are a primary source from which cracks originate. The adopted life prediction approach addresses this complexity by separating the coating and single crystal crack initiation regimes. This provides a flexible means for using different life model formulations for the coating and single crystal materials. At the completion of this program, all constitutive and life model formulations will be available in equation form and as software. The software will use the MARC general purpose finite element code to drive the constitutive models and calculate life parameters.

  8. Early stage fatigue damage occurs in bovine tendon fascicles in the absence of changes in mechanics at either the gross or micro-structural level.

    PubMed

    Shepherd, Jennifer H; Riley, Graham P; Screen, Hazel R C

    2014-10-01

    Many tendon injuries are believed to result from repetitive motion or overuse, leading to the accumulation of micro-damage over time. In vitro fatigue loading can be used to characterise damage during repeated use and investigate how this may relate to the aetiology of tendinopathy. This study considered the effect of fatigue loading on fascicles from two functionally distinct bovine tendons: the digital extensor and deep digital flexor. Micro-scale extension mechanisms were investigated in fascicles before or after a period of cyclic creep loading, comparing two different measurement techniques - the displacement of a photo-bleached grid and the use of nuclei as fiducial markers. Whilst visual damage was clearly identified after only 300 cycles of creep loading, these visual changes did not affect either gross fascicle mechanics or fascicle microstructural extension mechanisms over the 900 fatigue cycles investigated. However, significantly greater fibre sliding was measured when observing grid deformation rather than the analysis of nuclei movement. Measurement of microstructural extension with both techniques was localised and this may explain the absence of change in microstructural deformation in response to fatigue loading. Alternatively, the data may demonstrate that fascicles can withstand a degree of matrix disruption with no impact on mechanics. Whilst use of a photo-bleached grid to directly measure the collagen is the best indicator of matrix deformation, nuclei tracking may provide a better measure of the strain perceived directly by the cells. PMID:25001495

  9. Early stage fatigue damage occurs in bovine tendon fascicles in the absence of changes in mechanics at either the gross or micro-structural level

    PubMed Central

    Shepherd, Jennifer H.; Riley, Graham P.; Screen, Hazel R.C.

    2014-01-01

    Many tendon injuries are believed to result from repetitive motion or overuse, leading to the accumulation of micro-damage over time. In vitro fatigue loading can be used to characterise damage during repeated use and investigate how this may relate to the aetiology of tendinopathy. This study considered the effect of fatigue loading on fascicles from two functionally distinct bovine tendons: the digital extensor and deep digital flexor. Micro-scale extension mechanisms were investigated in fascicles before or after a period of cyclic creep loading, comparing two different measurement techniques – the displacement of a photo-bleached grid and the use of nuclei as fiducial markers. Whilst visual damage was clearly identified after only 300 cycles of creep loading, these visual changes did not affect either gross fascicle mechanics or fascicle microstructural extension mechanisms over the 900 fatigue cycles investigated. However, significantly greater fibre sliding was measured when observing grid deformation rather than the analysis of nuclei movement. Measurement of microstructural extension with both techniques was localised and this may explain the absence of change in microstructural deformation in response to fatigue loading. Alternatively, the data may demonstrate that fascicles can withstand a degree of matrix disruption with no impact on mechanics. Whilst use of a photo-bleached grid to directly measure the collagen is the best indicator of matrix deformation, nuclei tracking may provide a better measure of the strain perceived directly by the cells. PMID:25001495

  10. The influence of tensile fatigue damage on residual compressive strength of woven composites

    SciTech Connect

    Mitrovic, M.; Carman, G.P.

    1995-12-31

    The long term mechanical fatigue of a Celion G30-500/PMR-15 woven composite system is investigated to study the interrelationship between thermo-mechanical properties, namely the thermal expansion coefficient (TEC) and the compressive strength. Residual compressive strength measurements (IITRI fixture) conducted on specimens subjected to tension-tension fatigue cycling indicate that this material property is sensitive to cracks and delaminations which form during mechanical cycling. Measured compressive strength degradation are as large as 49% for this material undergoing mechanical fatigue cycling with TEC degradation as large as 61%. Experimental results show that a correlation exists between TEC measurements and compressive strength. This correlation suggests that TEC measurements may be used as a damage evaluation technique.

  11. Self-sealing of thermal fatigue and mechanical damage in fiber-reinforced composite materials

    NASA Astrophysics Data System (ADS)

    Moll, Jericho L.

    Fiber reinforced composite tanks provide a promising method of storage for liquid oxygen and hydrogen for aerospace applications. The inherent thermal fatigue of these vessels leads to the formation of microcracks, which allow gas phase leakage across the tank walls. In this dissertation, self-healing functionality is imparted to a structural composite to effectively seal microcracks induced by both mechanical and thermal loading cycles. Two different microencapsulated healing chemistries are investigated in woven glass fiber/epoxy and uni-weave carbon fiber/epoxy composites. Self-healing of mechanically induced damage was first studied in a room temperature cured plain weave E-glass/epoxy composite with encapsulated dicyclopentadiene (DCPD) monomer and wax protected Grubbs' catalyst healing components. A controlled amount of microcracking was introduced through cyclic indentation of opposing surfaces of the composite. The resulting damage zone was proportional to the indentation load. Healing was assessed through the use of a pressure cell apparatus to detect nitrogen flow through the thickness direction of the damaged composite. Successful healing resulted in a perfect seal, with no measurable gas flow. The effect of DCPD microcapsule size (51 microm and 18 microm) and concentration (0--12.2 wt%) on the self-sealing ability was investigated. Composite specimens with 6.5 wt% 51 microm capsules sealed 67% of the time, compared to 13% for the control panels without healing components. A thermally stable, dual microcapsule healing chemistry comprised of silanol terminated poly(dimethyl siloxane) plus a crosslinking agent and a tin catalyst was employed to allow higher composite processing temperatures. The microcapsules were incorporated into a satin weave E-glass fiber/epoxy composite processed at 120°C to yield a glass transition temperature of 127°C. Self-sealing ability after mechanical damage was assessed for different microcapsule sizees (25 microm and 42

  12. Influence of interface ply orientation on fatigue damage of adhesively bonded composite joints

    NASA Technical Reports Server (NTRS)

    Johnson, W. S.; Mall, S.

    1986-01-01

    An experimental study of cracked-lap-shear specimens was conducted to determine the influence of adherend stacking sequence on debond initiation and damage growth in a composite-to-composite bonded joint. Specimens consisted of quasi-isotropic graphite/epoxy adherends bonded together with either FM-300 or EC 3445 adhesives. The stacking sequence of the adherends was varied such that 0 deg, 45 deg, or 90 deg plies were present at the adherend-adhesive interfaces. Fatigue damage initiated in the adhesive layer in those specimens with 0 deg and 45 deg interface plies. Damaage initiated in the form of ply cracking in the strap adherend for the specimens with 90 deg interface plies. The fatigue-damage growth was in the form of delamination within the composite adherends for specimens with the 90 deg and 45 deg plies next to the adhesive, while debonding in the adhesive resulted for the specimens with 0 deg plies next to the adhesive. Those joints with the 0 deg and 45 deg plies next to either adhesive has essentially the same fatigue-damage-initiation stress levels. These stress levels were 13 and 71 percent higher, respectively, than those for specimens with 90 deg plies next to the EC 3445 and FM-300 adhesives.

  13. Acoustic emission and fatigue damage induced in plasma-sprayed hydroxyapatite coating layers.

    PubMed

    Laonapakul, Teerawat; Otsuka, Yuichi; Nimkerdphol, Achariya Rakngarm; Mutoh, Yoshiharu

    2012-04-01

    In order to improve the adhesive strength of hydroxyapatite (HAp) coatings, grit blasting with Al(2)O(3) powder and then wet blasting with HAp/Ti mixed powders was carried out on a commercially pure Ti (cp-Ti) substrate. Subsequently, an HAp/Ti bond coat layer and HAp top coat layer were deposited by plasma spraying. Fatigue tests of the HAp-coated specimens were carried out under four-point bending. Acoustic emission (AE) signals during the entire fatigue test were monitored to investigate the fatigue cracking behavior of the HAp-coated specimens. The HAp-coated specimens could survive up to 10(7) cycles without spallation of the HAp coating layers at the stress amplitude of 120 MPa. The HAp-coated specimens without HAp/Ti bond coat layer showed shorter fatigue life and easy crack nucleation compared to the HAp-coated specimens with HAp/Ti bond coat layer. The delamination and spallation of the HAp top coat with HAp/Ti bond coat on cp-Ti was not observed until the crack propagated into the cp-Ti during the final fracture stage of the fatigue cycle. Therefore, the HAp/Ti bond coat layer was found to greatly improve the fatigue damage resistance of the HAp coating layer. Three stages of the fatigue failure behavior of the HAp top coat with HAp/Ti bond coat on a cp-Ti substrate can be clearly estimated by the AE monitoring technique. These stages are cracks nucleating and propagating in the coating layer, cracks propagating in the substrate, and cracks propagating unstably to final fracture.

  14. Monitoring of fatigue damage in composite lap-joints using guided waves and FBG sensors

    NASA Astrophysics Data System (ADS)

    Karpenko, Oleksii; Khomenko, Anton; Koricho, Ermias; Haq, Mahmoodul; Udpa, Lalita

    2016-02-01

    Adhesive bonding is being increasingly employed in many applications as it offers possibility of light-weighting and efficient multi-material joining along with reduction in time and cost of manufacturing. However, failure initiation and progression in critical components like joints, specifically in fatigue loading is not well understood, which necessitates reliable NDE and SHM techniques to ensure structural integrity. In this work, concurrent guided wave (GW) and fiber Bragg grating (FBG) sensor measurements were used to monitor fatigue damage in adhesively bonded composite lap-joints. In the present set-up, one FBG sensor was strategically embedded in the adhesive bond-line of a lap-joint, while two other FBGs were bonded on the surface of the adherends. Full spectral responses of FBG sensors were collected and compared at specific intervals of fatigue loading. In parallel, guided waves were actuated and sensed using PZT wafers mounted on the composite adherends. Experimental results demonstrated that time-of-flight (ToF) of the fundamental modes transmitted through the bond-line and spectral response of FBG sensors were sensitive to fatigue loading and damage. Combination of guided wave and FBG measurements provided the desired redundancy and synergy in the data to evaluate the degradation in bond-line properties. Measurements taken in the presence of continuously applied load replicated the in-situ/service conditions. The approach shows promise in understanding the behavior of bonded joints subjected to complex loading.

  15. Damage Assessment of Heat Resistant Steels through Electron BackScatter Diffraction Strain Analysis under Creep and Creep-Fatigue Conditions

    NASA Astrophysics Data System (ADS)

    Fujiyama, Kazunari; Kimachi, Hirohisa; Tsuboi, Toshiki; Hagiwara, Hiroyuki; Ogino, Shotaro; Mizutani, Yoshiki

    EBSD(Electron BackScatter Diffraction) analyses were conducted for studying the quantitative microstructural metrics of creep and creep-fatigue damage for austenitic SUS304HTB boiler tube steel and ferritic Mod.9Cr piping steel. KAM(Kernel Average Misorientation) maps and GOS(Grain Orientation Spread) maps were obtained for these samples and the area averaged values KAMave and GOSave were obtained. While the increasing trends of these misorientation metrics were observed for SUS304HTB steel, the decreasing trends were observed for damaged Mod.9Cr steel with extensive recovery of subgrain structure. To establish more universal parameter representing the accumulation of damage to compensate these opposite trends, the EBSD strain parameters were introduced for converting the misorientation changes into the quantities representing accumulated permanent strains during creep and creep-fatigue damage process. As KAM values were dependent on the pixel size (inversely proportional to the observation magnification) and the permanent strain could be expressed as the shear strain which was the product of dislocation density, Burgers vector and dislocation movement distance, two KAM strain parameters MεKAMnet and MεδKAMave were introduced as the sum of product of the noise subtracted KAMnet and the absolute change from initial value δKAMave with dislocation movement distance divided by pixel size. MεδKAMave parameter showed better relationship both with creep strain in creep tests and accumulated creep strain range in creep-fatigue tests. This parameter can be used as the strain-based damage evaluation and detector of final failure.

  16. Effect of Buckling Modes on the Fatigue Life and Damage Tolerance of Stiffened Structures

    NASA Technical Reports Server (NTRS)

    Davila, Carlos G.; Bisagni, Chiara; Rose, Cheryl A.

    2015-01-01

    The postbuckling response and the collapse of composite specimens with a co-cured hat stringer are investigated experimentally and numerically. These specimens are designed to evaluate the postbuckling response and the effect of an embedded defect on the collapse load and the mode of failure. Tests performed using controlled conditions and detailed instrumentation demonstrate that the damage tolerance, fatigue life, and collapse loads are closely tied with the mode of the postbuckling deformation, which can be different between two nominally identical specimens. Modes that tend to open skin/stringer defects are the most damaging to the structure. However, skin/stringer bond defects can also propagate under shearing modes. In the proposed paper, the effects of initial shape imperfections on the postbuckling modes and the interaction between different postbuckling deformations and the propagation of skin/stringer bond defects under quasi-static or fatigue loads will be examined.

  17. Fatigue crack growth in damage tolerant Al-Li sheet alloys

    NASA Astrophysics Data System (ADS)

    Wanhill, R. J. H.

    1990-03-01

    The fatigue crack growth properties of two candidate damage tolerant Al-Li sheet alloys, 2091 and 8090 are compared with those of the conventional and widely used 2024 alloy. There were three load histories: constant amplitude, gust spectrum, and constant amplitude with occasional peak loads. The results are interpreted with the aid of fractographic observations and measurements of fracture surface roughness. The practical significance of the results is assessed, and recommendations are made for further evaluations.

  18. A continuum damage model of fatigue-induced damage in laminated composites

    NASA Technical Reports Server (NTRS)

    Harris, Charles E.; Allen, David H.

    1988-01-01

    A model is presented which predicts the stress-strain behavior of continuous fiber reinforced laminated composites in the presence of microstructural damage. The model is based on the concept of continuum damage mechanics and uses internal state variables to characterize the various damage modes. The associated internal state variable growth laws are mathematical models of the loading history induced development of microstructural damage. The model is demonstrated by using it to predict the response of damaged AS-4/3502 graphite/epoxy laminate panels.

  19. The strainrange conversion principle for treating cumulative fatigue damage in the creep range

    NASA Technical Reports Server (NTRS)

    Manson, S. S.

    1983-01-01

    A formula is derived for combining effects of successive hysteresis loops in the creep range of materials when one loop has excess tensile creep, while the other contains excess compressive creep. The resultant effect resembles single loops involving balanced tensile and compressive creep. The attempt to use the Interaction Damage Rule as a tool in combining loops of non-equal size and complex strainrange content has led to important new concepts useful in future studies of creep-fatigue. It turns out that the Interaction Damage Rule is basically an expression of how a set of hysteresis loops involving only single generic strains can combine to produce the same micromechanistic damage as the loop containing the combined strainranges which it analyzes. Making use of the underlying concept of Strainrange Partitioning that only the strainrange content of a hysteresis loop governs fatigue life, not order of introducing strainranges, a rational derivation of the Interaction Damage Rule is provided, showing also how it can effectively be used to synthesize independent loops and determine both damaging and healing effects.

  20. Model-Based Fatigue Prognosis of Fiber-Reinforced Laminates Exhibiting Concurrent Damage Mechanisms

    NASA Technical Reports Server (NTRS)

    Corbetta, M.; Sbarufatti, C.; Saxena, A.; Giglio, M.; Goebel, K.

    2016-01-01

    Prognostics of large composite structures is a topic of increasing interest in the field of structural health monitoring for aerospace, civil, and mechanical systems. Along with recent advancements in real-time structural health data acquisition and processing for damage detection and characterization, model-based stochastic methods for life prediction are showing promising results in the literature. Among various model-based approaches, particle-filtering algorithms are particularly capable in coping with uncertainties associated with the process. These include uncertainties about information on the damage extent and the inherent uncertainties of the damage propagation process. Some efforts have shown successful applications of particle filtering-based frameworks for predicting the matrix crack evolution and structural stiffness degradation caused by repetitive fatigue loads. Effects of other damage modes such as delamination, however, are not incorporated in these works. It is well established that delamination and matrix cracks not only co-exist in most laminate structures during the fatigue degradation process but also affect each other's progression. Furthermore, delamination significantly alters the stress-state in the laminates and accelerates the material degradation leading to catastrophic failure. Therefore, the work presented herein proposes a particle filtering-based framework for predicting a structure's remaining useful life with consideration of multiple co-existing damage-mechanisms. The framework uses an energy-based model from the composite modeling literature. The multiple damage-mode model has been shown to suitably estimate the energy release rate of cross-ply laminates as affected by matrix cracks and delamination modes. The model is also able to estimate the reduction in stiffness of the damaged laminate. This information is then used in the algorithms for life prediction capabilities. First, a brief summary of the energy-based damage model

  1. Modeling of long-term fatigue damage of soft tissue with stress softening and permanent set effects

    PubMed Central

    Martin, Caitlin; Sun, Wei

    2012-01-01

    One of the major failure modes of bioprosthetic heart valves is non-calcific structural deterioration due to fatigue of the tissue leaflets. Experimental methods to characterize tissue fatigue properties are complex and time-consuming. A constitutive fatigue model that could be calibrated by isolated material tests would be ideal for investigating the effects of more complex loading conditions. However, there is a lack of tissue fatigue damage models in the literature. To address these limitations, in this study, a phenomenological constitutive model was developed to describe the stress softening and permanent set effects of tissue subjected to long-term cyclic loading. The model was used to capture characteristic uniaxial fatigue data for glutaraldehyde-treated bovine pericardium and was then implemented into finite element software. The simulated fatigue response agreed well with the experimental data and thus demonstrates feasibility of this approach. PMID:22945802

  2. Damage formation, fatigue behavior and strength properties of ZrO2-based ceramics

    NASA Astrophysics Data System (ADS)

    Kozulin, A. A.; Narikovich, A. S.; Kulkov, S. N.; Leitsin, V. N.; Kulkov, S. S.

    2016-08-01

    It is suggested that a non-destructive testing technique using a three-dimensional X-ray tomography be applied to detecting internal structural defects and monitoring damage formation in a ceramic composite structure subjected to a bending load. Three-point bending tests are used to investigate the fatigue behavior and mechanical and physical properties of medical-grade ZrO2-based ceramics. The bending strength and flexural modulus are derived under static conditions at a loading rate of 2 mm/min. The fatigue strength and fatigue limit under dynamic loading are investigated at a frequency of 10 Hz in three stress ranges: 0.91-0.98, 0.8-0.83, and 0.73-0.77 MPa of the static bending strength. The average values of the bending strength and flexural modulus of sintered specimens are 43 MPa and 22 GPa, respectively. The mechanical properties of the ceramics are found to be similar to those of bone tissues. The testing results lead us to conclude that the fatigue limit obtained from 105 stress cycles is in the range 33-34 MPa, i.e. it accounts for about 75% of the static bending strength for the test material.

  3. Fatigue

    MedlinePlus

    Bennett RM. Fibromyalgia, chronic fatigue syndrome, and myofascial pain. In: Goldman L, Schafer AI, eds. Goldman's Cecil Medicine . 25th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 274. Thames TA, Karrh ...

  4. Damage repair in CMSX-4 alloy without fatigue life reduction penalty

    NASA Astrophysics Data System (ADS)

    Okazaki, Masakazu; Ohtera, Issei; Harada, Yoshio

    2004-02-01

    The microstructural changes in a single-crystal Ni-base superalloy, CMSX-4, that might occur during the processes of repair and recoating of hot section components for advanced gas turbines were studied. It is shown that the cellular γ/γ‧ microstructure is formed when the material is subjected to local plastic straining, followed by the reheat treatments during the course of damage recovery. The formation of cellular microstructure in the material led to the remarkably reduced fatigue strength. In order to reduce or prevent the preceding undesirable effect resulting from cellular microstructure, a new method based on applying overlay coating technique was developed. The method is based on an idea that the alloying elements that are depleted in base alloys could be supplemented via the overlay coating. An X alloy, which contains grain boundary strengthening elements, was selected and coated on the CMSX-4 with the cellular microstructure by low-pressure plasma spraying. The fatigue tests on the coated CMSX-4 specimens demonstrated the effectiveness of the method. The observations of the crack initiation site, the fatigue fracture mode, the crack density in the cellular transformed area, and the crack propagation morphologies near the prior interface strongly supported the validity of this approach. The method is expected to build a road to a so-called damage cure (or recovery) coating.

  5. Gear Fault Detection Effectiveness as Applied to Tooth Surface Pitting Fatigue Damage

    NASA Technical Reports Server (NTRS)

    Lewicki, David G.; Dempsey, Paula J.; Heath, Gregory F.; Shanthakumaran, Perumal

    2009-01-01

    A study was performed to evaluate fault detection effectiveness as applied to gear tooth pitting fatigue damage. Vibration and oil-debris monitoring (ODM) data were gathered from 24 sets of spur pinion and face gears run during a previous endurance evaluation study. Three common condition indicators (RMS, FM4, and NA4) were deduced from the time-averaged vibration data and used with the ODM to evaluate their performance for gear fault detection. The NA4 parameter showed to be a very good condition indicator for the detection of gear tooth surface pitting failures. The FM4 and RMS parameters performed average to below average in detection of gear tooth surface pitting failures. The ODM sensor was successful in detecting a significant amount of debris from all the gear tooth pitting fatigue failures. Excluding outliers, the average cumulative mass at the end of a test was 40 mg.

  6. Performance optimization of a diagnostic system based upon a simulated strain field for fatigue damage characterization

    NASA Astrophysics Data System (ADS)

    Sbarufatti, C.; Manes, A.; Giglio, M.

    2013-11-01

    The work presented hereafter is about the development of a diagnostic system for crack damage detection, localization and quantification on a typical metallic aeronautical structure (skin stiffened through riveted stringers). Crack detection and characterization are based upon strain field sensitivity to damage. The structural diagnosis is carried out by a dedicated smart algorithm (Artificial Neural Network) which is trained on a database of Finite Element simulations relative to damaged and undamaged conditions, providing the system with an accurate predictor at low overall cost. The algorithm, trained on numerical damage experience, is used in a simulated environment to provide reliable preliminary information concerning the algorithm performances for damage diagnosis, thus further reducing the experimental costs and efforts associated with the development and optimization of such systems. The same algorithm has been tested on real experimental strain patterns acquired during real fatigue crack propagation, thus verifying the capability of the numerically trained algorithm for anomaly detection, damage assessment and localization on a real complex structure. The load variability, the discrepancy between the Finite Element Model and the real structure, and the uncertainty in the algorithm training process have been addressed in order to enhance the robustness of the system inference process. Some further algorithm training strategies are discussed, aimed at minimizing the risk for false alarms while maintaining a high probability of damage detection.

  7. Continuum Fatigue Damage Modeling for Critical Design, Control, and Fault Prognosis

    NASA Technical Reports Server (NTRS)

    Lorenzo, Carl F.

    1996-01-01

    This paper develops a simplified continuum (continuous with respect to time, stress, etc.) fatigue damage model for use in critical design, Life Extending Control and fault prognosis. The work is based on the local strain cyclic damage modeling method. New nonlinear explicit equation forms of cyclic damage in terms of stress amplitude are derived to facilitate the continuum modelling. Stress based continuum models are derived. Extension to plastic strain-strain rate models is also presented. Progress toward a non-zero mean stress based is presented. Also new nonlinear explicit equation forms in terms of stress amplitude are derived for this case. Application of the various models to design, control, and fault prognosis is considered.

  8. Investigation of Bearing Fatigue Damage Life Prediction Using Oil Debris Monitoring

    NASA Technical Reports Server (NTRS)

    Dempsey, Paula J.; Bolander, Nathan; Haynes, Chris; Toms, Allison M.

    2011-01-01

    Research was performed to determine if a diagnostic tool for detecting fatigue damage of helicopter tapered roller bearings can be used to determine remaining useful life (RUL). The taper roller bearings under study were installed on the tail gearbox (TGB) output shaft of UH- 60M helicopters, removed from the helicopters and subsequently installed in a bearing spall propagation test rig. The diagnostic tool was developed and evaluated experimentally by collecting oil debris data during spall progression tests on four bearings. During each test, data from an on-line, in-line, inductance type oil debris sensor was monitored and recorded for the occurrence of pitting damage. Results from the four bearings tested indicate that measuring the debris generated when a bearing outer race begins to spall can be used to indicate bearing damage progression and remaining bearing life.

  9. Unlimited damage accumulation in metallic materials under cascade-damage conditions

    SciTech Connect

    Barashev, Aleksandr; Golubov, Stanislav I

    2009-01-01

    Most experiments on neutron or heavy-ion cascade-produced irradiation of pure metals and metallic alloys demonstrate continuous unlimited void growth as well as development of the dislocation structure. In contrast, the theory of radiation damage predicts saturation of void swelling at sufficiently high irradiation doses and according termination of accumulation of interstitial-type defects. It is shown in the present paper that, in the conditions of steady production of one-dimensionally (1-D) mobile clusters of self-interstitial atoms (SIAs) in displacement cascades, three reasons can be behind these observations. First, if the fraction of SIAs generated in the clustered form is smaller than some finite value of the order of the dislocation bias factor. Second, if solute, impurity or transmuted atoms segregate to voids and repel the SIA clusters. Third, if spatial correlations between voids and other defects, such as second-phase precipitates and dislocations, establish that provide shadowing of voids from the SIA clusters. The driving force for the development of such correlations is the same as for the void lattice formation and is argued to be always present under cascade-damage conditions. It is emphasized that the mean-free path of 1-D migrating SIA clusters is typically at least an order of magnitude longer than the average distance between microstructural defects; hence spatial correlations on the same scale should be taken into consideration. A way of developing a predictive theory is discussed. An interpretation of the universal steady-state swelling rate of ~1%/dpa observed in austenitic steels is proposed for the first time.

  10. Characterization of Fatigue Damage for Bonded Composite Skin/Stringer Configurations

    NASA Technical Reports Server (NTRS)

    Paris, Isabelle; Cvitkovich, Michael; Krueger, Ronald

    2008-01-01

    The fatigue damage was characterized in specimens which consisted of a tapered composite flange bonded onto a composite skin. Quasi-static tension tests were performed first to determine the failure load. Subsequently, tension fatigue tests were performed at 40%, 50%, 60% and 70% of the failure load to evaluate the debonding mechanisms. For four specimens, the cycling loading was stopped at intervals. Photographs of the polished specimen edges were taken under a light microscope to document the damage. At two diagonally opposite corners of the flange, a delamination appeared to initiate at the flange tip from a matrix crack in the top 45deg skin ply and propagated at the top 45deg/-45deg skin ply interface. At the other two diagonally opposite corners, a delamination running in the bondline initiated from a matrix crack in the adhesive pocket. In addition, two specimens were cut longitudinally into several sections. Micrographs revealed a more complex pattern inside the specimen where the two delamination patterns observed at the edges are present simultaneously across most of the width of the specimen. The observations suggest that a more sophisticated nondestructive evaluation technique is required to capture the complex damage pattern of matrix cracking and multi-level delaminations.

  11. Multitechnique monitoring of fatigue damage in adhesively bonded composite lap-joints

    NASA Astrophysics Data System (ADS)

    Karpenko, Oleksii; Koricho, Ermias; Khomenko, Anton; Dib, Gerges; Haq, Mahmoodul; Udpa, Lalita

    2015-03-01

    The requirement for reduced structural weight has driven the development of adhesively bonded joints. However, a major issue preventing their full acceptance is the initiation of premature failure in the form of a disbond between adherends, mainly due to fatigue, manufacturing flaws or impact damage. This work presents the integrated approach for in-situ monitoring of degradation of the adhesive bond in the GFRP composite lap-joint using ultrasonic guided waves and dynamic measurements from strategically embedded FBG sensors. Guided waves are actuated with surface mounted piezoelectric elements and mode tuning is used to provide high sensitivity to the degradation of the adhesive layer parameters. Composite lap-joints are subjected to fatigue loading, and data from piezoceramic transducers are collected at regular intervals to evaluate the progression of damage. Results demonstrate that quasi-static loading affects guided wave measurements considerably, but FBG sensors can be used to monitor the applied load levels and residual strains in the adhesive bond. The proposed technique shows promise for determining the post-damage stiffness of adhesively bonded joints.

  12. Monitoring Thermal Fatigue Damage In Nuclear Power Plant Materials Using Acoustic Emission

    SciTech Connect

    Meyer, Ryan M.; Ramuhalli, Pradeep; Watson, Bruce E.; Pitman, Stan G.; Roosendaal, Timothy J.; Bond, Leonard J.

    2012-04-26

    Proactive aging management of nuclear power plant passive components requires technologies to enable monitoring and accurate quantification of material condition at early stages of degradation (i.e., pre-macrocrack). Acoustic emission (AE) is well-suited to continuous monitoring of component degradation and is proposed as a method to monitor degradation during accelerated thermal fatigue tests. A key consideration is the ability to separate degradation responses from external sources such as water spray induced during thermal fatigue testing. Water spray provides a significant background of acoustic signals, which can overwhelm AE signals caused by degradation. Analysis of AE signal frequency and energy is proposed in this work as a means for separating degradation signals from background sources. Encouraging results were obtained by applying both frequency and energy filters to preliminary data. The analysis of signals filtered using frequency and energy provides signatures exhibiting several characteristics that are consistent with degradation accumulation in materials. Future work is planned to enable verification of the efficacy of AE for thermal fatigue crack initiation detection. While the emphasis has been placed on the use of AE for crack initiation detection during accelerated aging tests, this work also has implications with respect to the use of AE as a primary tool for early degradation monitoring in nuclear power plant materials. The development of NDE tools for characterization of aging in materials can also benefit from the use of a technology such as AE which can continuously monitor and detect crack initiation during accelerated aging tests.

  13. Room Temperature Tensile Behavior and Damage Accumulation of Hi-Nicalon Reinforced SiC Matrix Composites

    NASA Technical Reports Server (NTRS)

    Morscher, G. N.; Gyekenyesi, J. Z.

    1998-01-01

    Composites consisting of woven Hi-Nicalon fibers, BN interphases, and different SiC matrices were studied in tension at room temperature. Composites with SiC matrices processed by CVI and melt infiltration were compared. Monotonic and load/unload/reload tensile hysteresis experiments were performed. A modal acoustic emission (AE) analyzer was used to monitor damage accumulation during the tensile test. Post test polishing of the tensile gage sections was performed to determine the extent of cracking. The occurrence and location of cracking could easily be determined using modal AE. The loss of modulus could also effectively be determined from the change in the velocity of sound across the sample. Finally, the stresses where cracks appear to intersect the load-bearing fibers correspond with high temperature low cycle fatigue run out stresses for these materials.

  14. Direct evidence of "damage accumulation" in cement mantles surrounding femoral hip stems retrieved at autopsy: cement damage correlates with duration of use and BMI.

    PubMed

    Race, A; Miller, M A; Izant, T H; Mann, K A

    2011-09-01

    The "damage accumulation" phenomenon has not been quantitatively demonstrated in clinical cement mantles surrounding femoral hip stems. We stained transverse sections of 11 postmortem retrieved femoral hip components fixed with cement using fluorescent dye-penetrant and quantified cement damage, voids, and cement-bone interface gaps in epifluorescence and white light micrographs. Crack density (Cr.Dn), crack length-density (Cr.Ln.Dn), porosity, and cement-bone interface gap fraction (c/b-gap%) were calculated, normalized by mantle area. Multiple regression tests showed that cement damage (Cr.Ln.Dn. & Cr.Dn.) was significantly positively correlated (r(2)=0.98, p<0.001) with "duration of use" and body mass index ("BMI") but not cement mantle "porosity". There were significant interactions: "duration of use"*"BMI" was strongly predictive (p<0.005) of Cr.Dn.; and "duration of use"*"porosity" was predictive (p=0.04) of Cr.Ln.Dn. Stem related cracks accounted for approximately one fifth of Cr.Dn and one third of Cr.Ln.Dn. The mean c/b-gap% was 13.8% but it did not correlate (r(2)=0.01, p=0.8) with duration of use. We concluded that duration-dependent fatigue damage accumulation occurred during in vivo use. BMI strongly influenced cement crack length and the rate of new crack formation over time. Voids did not increase the rate of crack initiation but appeared to have promoted crack growth over time. Although not progressive, substantial bone resorption at the cement-bone interface appeared to be common.

  15. An overview of elevated temperature damage mechanisms and fatigue behavior of a unidirectional SCS-6/Ti-15-3 composite

    NASA Technical Reports Server (NTRS)

    Castelli, Michael G.; Gayda, John

    1993-01-01

    The fatigue behavior of a unidirectionally reinforced titanium matrix composite (TMC), SiC/Ti-15-3, was thoroughly characterized to support life prediction modeling of advanced TMC disks designed for gas turbine engine applications. The results of this coupon-level experimental investigation are reviewed. On a stress basis, the isothermal fatigue behavior of the (0 deg) TMC revealed significant improvements over the unreinforced matrix. In contrast, the (90 deg) TMC exhibited degraded properties and lives for similar comparisons. This was attributed to the weak fiber/matrix interfacial bond. Encasing the (0 deg) TMC with a Ti-15-3 case did not affect isothermal fatigue lives at higher strain levels. However, at lower strain levels, rapid initiation and propagation of large fatigue cracks in the case degraded the fatigue lives. Thermomechanical fatigue (TMF) lives were significantly reduced for the (0 deg) TMC when compared to isothermal lives. At high strains, in-phase TMF produced extremely short lives. This degradation was attributed to fiber overload failures brought about by stress relaxation in the matrix. At low strains, out-of-phase TMF conditions became life limiting. Environment-assisted surface cracking was found to accelerate fatigue failure. This produced extensive matrix damage with minimal fiber damage. For the (90 deg) TMC, TMF conditions did not promote an additional degradation in cyclic life beyond that observed under isothermal conditions.

  16. Monitoring Damage Accumulation in Ceramic Matrix Composites Using Electrical Resistivity

    NASA Technical Reports Server (NTRS)

    Smith, Craig E.; Morscher, Gregory N.; Xia, Zhenhai H.

    2008-01-01

    The electric resistance of woven SiC fiber reinforced SiC matrix composites were measured under tensile loading conditions. The results show that the electrical resistance is closely related to damage and that real-time information about the damage state can be obtained through monitoring of the resistance. Such self-sensing capability provides the possibility of on-board/in-situ damage detection and accurate life prediction for high-temperature ceramic matrix composites. Woven silicon carbide fiber-reinforced silicon carbide (SiC/SiC) ceramic matrix composites (CMC) possess unique properties such as high thermal conductivity, excellent creep resistance, improved toughness, and good environmental stability (oxidation resistance), making them particularly suitable for hot structure applications. In specific, CMCs could be applied to hot section components of gas turbines [1], aerojet engines [2], thermal protection systems [3], and hot control surfaces [4]. The benefits of implementing these materials include reduced cooling air requirements, lower weight, simpler component design, longer service life, and higher thrust [5]. It has been identified in NASA High Speed Research (HSR) program that the SiC/SiC CMC has the most promise for high temperature, high oxidation applications [6]. One of the critical issues in the successful application of CMCs is on-board or insitu assessment of the damage state and an accurate prediction of the remaining service life of a particular component. This is of great concern, since most CMC components envisioned for aerospace applications will be exposed to harsh environments and play a key role in the vehicle s safety. On-line health monitoring can enable prediction of remaining life; thus resulting in improved safety and reliability of structural components. Monitoring can also allow for appropriate corrections to be made in real time, therefore leading to the prevention of catastrophic failures. Most conventional nondestructive

  17. Micromechanics Fatigue Damage Analysis Modeling for Fabric Reinforced Ceramic Matrix Composites

    NASA Technical Reports Server (NTRS)

    Min, J. B.; Xue, D.; Shi, Y.

    2013-01-01

    A micromechanics analysis modeling method was developed to analyze the damage progression and fatigue failure of fabric reinforced composite structures, especially for the brittle ceramic matrix material composites. A repeating unit cell concept of fabric reinforced composites was used to represent the global composite structure. The thermal and mechanical properties of the repeating unit cell were considered as the same as those of the global composite structure. The three-phase micromechanics, the shear-lag, and the continuum fracture mechanics models were integrated with a statistical model in the repeating unit cell to predict the progressive damages and fatigue life of the composite structures. The global structure failure was defined as the loss of loading capability of the repeating unit cell, which depends on the stiffness reduction due to material slice failures and nonlinear material properties in the repeating unit cell. The present methodology is demonstrated with the analysis results evaluated through the experimental test performed with carbon fiber reinforced silicon carbide matrix plain weave composite specimens.

  18. Fatigue and damage tolerance of Y-TZP ceramics in layered biomechanical systems.

    PubMed

    Zhang, Yu; Pajares, Antonia; Lawn, Brian R

    2004-10-15

    The fatigue properties of fine-grain Y-TZP in cyclic flexural testing are studied. Comparative tests on a coarser-grain alumina provide a baseline control. A bilayer configuration with ceramic plates bonded to a compliant polymeric substrate and loaded with concentrated forces at the top surfaces, simulating basic layer structures in dental crowns and hip replacement prostheses, is used as a basic test specimen. Critical times to initiate radial crack failure at the ceramic undersurfaces at prescribed maximum surface loads are measured for Y-TZP with as-polished surfaces, mechanically predamaged undersurfaces, and after a thermal aging treatment. No differences in critical failure conditions are observed between monotonic and cyclic loading on as-polished surfaces, or between as-polished and mechanically damaged surfaces in monotonic loading, consistent with fatigue controlled by slow crack growth. However, the data for mechanically damaged and aged specimens show substantial declines in sustainable stresses and times to failure in cyclic loading, indicating an augmenting role of mechanical and thermal processes in certain instances. In all cases, however, the sustainable stresses in the Y-TZP remain higher than that of the alumina, suggesting that with proper measures to avoid inherent structural instabilities, Y-TZP could provide superior performance in biomechanical applications.

  19. Neural Network Modelling of Oscillatory Loads and Fatigue Damage Estimation of Helicopter Components

    NASA Astrophysics Data System (ADS)

    Cabell, R. H.; Fuller, C. R.; O'Brien, W. F.

    1998-01-01

    A neural network for the prediction of oscillatory loads used for on-line health monitoring of flight critical components in an AH-64A helicopter is described. The neural network is used to demonstrate the potential for estimating loads in the rotor system from fixed-system information. Estimates of the range of the pitch link load are determined by the neural network from roll, pitch, and yaw rates, airspeed, and other fixed-system information measured by the flight control computer on the helicopter. The predicted load range is then used to estimate fatigue damage to the pitch link. Actual flight loads data from an AH-64A helicopter are used to demonstrate the process. The predicted load ranges agree well with measured values for both training and test data. A linear model is also used to predict the load ranges, and its accuracy is noticeably worse than that of the neural network, especially at higher load values that cause fatigue damage. This demonstrates the necessity of the non-linear modelling capabilities of the neural network for this problem.

  20. Combining Passive Thermography and Acoustic Emission for Large Area Fatigue Damage Growth Assessment of a Composite Structure

    NASA Technical Reports Server (NTRS)

    Zalameda, Joseph N.; Horne, Michael R.; Madaras, Eric I.; Burke, Eric R.

    2016-01-01

    Passive thermography and acoustic emission data were obtained for improved real time damage detection during fatigue loading. A strong positive correlation was demonstrated between acoustic energy event location and thermal heating, especially if the structure under load was nearing ultimate failure. An image processing routine was developed to map the acoustic emission data onto the thermal imagery. This required removing optical barrel distortion and angular rotation from the thermal data. The acoustic emission data were then mapped onto thermal data, revealing the cluster of acoustic emission event locations around the thermal signatures of interest. By combining both techniques, progression of damage growth is confirmed and areas of failure are identified. This technology provides improved real time inspections of advanced composite structures during fatigue testing.Keywords: Thermal nondestructive evaluation, fatigue damage detection, aerospace composite inspection, acoustic emission, passive thermography

  1. Analytical modeling of contact acoustic nonlinearity of guided waves and its application to evaluating severity of fatigue damage

    NASA Astrophysics Data System (ADS)

    Wang, Kai; Su, Zhongqing

    2016-04-01

    Targeting quantitative estimate of fatigue damage, a dedicated analytical model was developed based on the modal decomposition method and the variational principle. The model well interprets the contact acoustic nonlinearity induced by a "breathing" crack in a two-dimensional scenario, and the nonlinear characteristics of guided ultrasonic waves (GUWs) (e.g., reflection, transmission, mode conversion and high-order generation) when GUWs traversing the crack. Based on the model, a second-order reflection index was defined. Using the index, a fatigue damage evaluation framework was established, showing demonstrated capacity of estimating the severity of fatigue damage in a quantitative manner. The approach, in principle, does not entail a benchmarking process against baseline signals pre-acquired from pristine counterparts. The results obtained using the analytical modeling were compared with those from finite element simulation, showing good coincidence. Limitations of the model were also discussed.

  2. Experimental Investigations on Fatigue Damage and Residual Properties of Interacting Notched Woven E-Glass/Epoxy Composite

    NASA Astrophysics Data System (ADS)

    Bhaskara Rao, Pathakokila; Rama Krishna, Avasarala; Ramji, Koona; Satya Devi, Ambadipudi

    2015-10-01

    The interacting notched laminates of plain weave E-glass fiber reinforced with epoxy were fatigued at predetermined frequency in tension-tension to investigate the fatigue damage and residual properties. The results from stress-life curves summarize that damage growing around the notches due to stress concentration is the underlying cause for the variation in fatigue strengths among the geometrically different specimens considered. The residual strength and modulus decay with respect to cycle number at 50 % of the ultimate tensile strength were investigated. It is evident from the experimental data that the residual strength decreases with cycle number and increases due to redistribution of stress around the notches. The detailed study of the damage development under cyclic loads also explains the causes of modulus reduction for all the laminate geometries.

  3. Accumulation of DNA damages in aging Paramecium tetraurelia.

    PubMed

    Holmes, G E; Holmes, N R

    1986-07-01

    Paramecium tetraurelia cells of ages 4, 15, and 27 days were labeled with [14C]-thymidine. In addition, cells were grown clonally for 27 days (108 generations) and labeled with [14C]-thymidine in the presence of 0.5 or 7.5 micrograms/ml of mitomycin-C (MMC) or no MMC. These cells were gently deposited on a filter membrane, which impedes the passage of DNA strands. The cells were then lysed with detergents and the cellular components washed through the filters, leaving double-stranded DNA intact on the surface. Proteinase K was used to remove histone or DNA-bound proteins. The DNA was then eluted under alkaline conditions, which denatures double-stranded DNA and converts apurinic/apyrimidinic sites into single-strand breaks. The results obtained with the cells of ages 4, 15, and 27 days (16, 60, and 108 generations, respectively) indicate that as Paramecium tetraurelia ages during asexual reproduction, apurinic/apyrimidinic lesions, strand breaks or single-strand gaps accumulate. This accumulation may be the basic mechanism of aging in such cells. In the MMC-treated cells of 27 days (108 generations), the MMC reduced elution of DNA fragments more at the higher than at the lower pH's used; random MMC cross-links should occur more often in longer strands than in shorter strands. The reductions in elution preferentially at higher pH, at which longer single strands would be eluted, confirmed the pH-versus-length relationship for Paramecium DNA eluted under our conditions. PMID:3091993

  4. Applications of nonequilibrium melting concept to damage-accumulation processes

    SciTech Connect

    Lam, N.Q.; Okamoto, P.R.

    1998-01-01

    The authors recent study of crystalline-to-amorphous transformation led to the successful development of a unified thermodynamic description of disorder-induced amorphization and heat-induced melting, based on a generalized version of the Lindemann melting criterion. The generalized criterion requires that the melting temperature of a defective crystal decreases with increasing static atomic disorder. Hence, any crystal can melt at temperatures below the melting point of its perfect crystalline state when driven far from equilibrium by introducing critical amounts of misfitting solute atoms and lattice imperfections, radiation damage, and/or tensile stresses. This conceptual approach to nonequilibrium melting provides new insight into long-standing materials problems such as brittle fracture, embrittlement, and environmentally-induced cracking, for example irradiation-assisted stress corrosion cracking.

  5. Muscle Damage and Its Relationship with Muscle Fatigue During a Half-Iron Triathlon

    PubMed Central

    Coso, Juan Del; González-Millán, Cristina; Salinero, Juan José; Abián-Vicén, Javier; Soriano, Lidón; Garde, Sergio; Pérez-González, Benito

    2012-01-01

    Background To investigate the cause/s of muscle fatigue experienced during a half-iron distance triathlon. Methodology/Principal Findings We recruited 25 trained triathletes (36±7 yr; 75.1±9.8 kg) for the study. Before and just after the race, jump height and leg muscle power output were measured during a countermovement jump on a force platform to determine leg muscle fatigue. Body weight, handgrip maximal force and blood and urine samples were also obtained before and after the race. Blood myoglobin and creatine kinase concentrations were determined as markers of muscle damage. Results Jump height (from 30.3±5.0 to 23.4±6.4 cm; P<0.05) and leg power output (from 25.6±2.9 to 20.7±4.6 W · kg−1; P<0.05) were significantly reduced after the race. However, handgrip maximal force was unaffected by the race (430±59 to 430±62 N). Mean dehydration after the race was 2.3±1.2% with high inter-individual variability in the responses. Blood myoglobin and creatine kinase concentration increased to 516±248 µg · L−1 and 442±204 U · L−1, respectively (P<0.05) after the race. Pre- to post-race jump change did not correlate with dehydration (r = 0.16; P>0.05) but significantly correlated with myoglobin concentration (r = 0.65; P<0.001) and creatine kinase concentration (r = 0.54; P<0.001). Conclusions/significance During a half-iron distance triathlon, the capacity of leg muscles to produce force was notably diminished while arm muscle force output remained unaffected. Leg muscle fatigue was correlated with blood markers of muscle damage suggesting that muscle breakdown is one of the most relevant sources of muscle fatigue during a triathlon. PMID:22900101

  6. Noncontact acousto-thermal evaluation of evolving fatigue damage in polycrystalline Ti-6Al-4V

    NASA Astrophysics Data System (ADS)

    Sathish, S.; Welter, J. T.; Schehl, N.; Jata, K. V.

    2014-05-01

    Non-Contact Acousto-Thermal Signature (NCATS) analysis uses conversion of acoustic energy to heat to characterize evolving damage in materials. In the past, the observed temperature changes were interpreted using phenomenological approaches. This paper presents details of the mechanisms and the theoretical models to predict the temperature change due to conversion of acoustic energy to heat. NCATS experimental measurements performed using 20 kHz high amplitude acoustic waves on as received and fatigued polycrystalline Ti-6Al-4V are compared with theoretical calculations based on the mechanisms of transverse thermal currents, inter-crystalline thermal currents, and dislocation density changes. In the as received samples, the transverse thermal currents contribution has been found to be negligible compared with inter-crystalline thermal currents contribution. The experimentally measured maximum temperature change in the as received sample has been found to be 0.5 °C, and the theoretical prediction based on inter-crystalline thermal currents is 0.08 °C. In the fatigue damaged samples, the maximum temperature change increases with increasing damage that can be attributed to the increasing dislocation density. The theoretical prediction of the maximum temperature attained by a sample that is near failure based on dislocation contribution is 2.0 °C, while the experimental measurements have been found to be 0.95 °C. The differences between the theoretical and the experimental measurements are discussed in the context of the uncertainties in several physical parameters used in the theoretical calculations.

  7. Damage Monitoring of Unidirectional C/SiC Ceramic-Matrix Composite under Cyclic Fatigue Loading using A Hysteresis Loss Energy-Based Damage Parameter at Room and Elevated Temperatures

    NASA Astrophysics Data System (ADS)

    Longbiao, Li

    2016-06-01

    The damage evolution of unidirectional C/SiC ceramic-matrix composite (CMC) under cyclic fatigue loading has been investigated using a hysteresis loss energy-based damage parameter at room and elevated temperatures. The experimental fatigue hysteresis modulus and fatigue hysteresis loss energy versus cycle number have been analyzed. By comparing the experimental fatigue hysteresis loss energy with theoretical computational values, the interface shear stress corresponding to different cycle number and peak stress has been estimated. The experimental evolution of fatigue hysteresis loss energy and fatigue hysteresis loss energy-based damage parameter versus cycle number has been predicted for unidirectional C/SiC composite at room and elevated temperatures. The predicted results of interface shear stress degradation, stress-strain hysteresis loops corresponding to different number of applied cycles, fatigue hysteresis loss energy and fatigue hysteresis loss energy-based damage parameter as a functions of cycle number agreed with experimental data. It was found that the fatigue hysteresis energy-based parameter can be used to monitor the fatigue damage evolution and predict the fatigue life of fiber-reinforced CMCs.

  8. Experimental Verification of a Progressive Damage Model for IM7/5260 Laminates Subjected to Tension-Tension Fatigue

    NASA Technical Reports Server (NTRS)

    Coats, Timothy W.; Harris, Charles E.

    1995-01-01

    The durability and damage tolerance of laminated composites are critical design considerations for airframe composite structures. Therefore, the ability to model damage initiation and growth and predict the life of laminated composites is necessary to achieve structurally efficient and economical designs. The purpose of this research is to experimentally verify the application of a continuum damage model to predict progressive damage development in a toughened material system. Damage due to monotonic and tension-tension fatigue was documented for IM7/5260 graphite/bismaleimide laminates. Crack density and delamination surface area were used to calculate matrix cracking and delamination internal state variables to predict stiffness loss in unnotched laminates. A damage dependent finite element code predicted the stiffness loss for notched laminates with good agreement to experimental data. It was concluded that the continuum damage model can adequately predict matrix damage progression in notched and unnotched laminates as a function of loading history and laminate stacking sequence.

  9. Gear Fault Detection Effectiveness as Applied to Tooth Surface Pitting Fatigue Damage

    NASA Technical Reports Server (NTRS)

    Lewicki, David G.; Dempsey, Paula J.; Heath, Gregory F.; Shanthakumaran, Perumal

    2010-01-01

    A study was performed to evaluate fault detection effectiveness as applied to gear-tooth-pitting-fatigue damage. Vibration and oil-debris monitoring (ODM) data were gathered from 24 sets of spur pinion and face gears run during a previous endurance evaluation study. Three common condition indicators (RMS, FM4, and NA4 [Ed. 's note: See Appendix A-Definitions D were deduced from the time-averaged vibration data and used with the ODM to evaluate their performance for gear fault detection. The NA4 parameter showed to be a very good condition indicator for the detection of gear tooth surface pitting failures. The FM4 and RMS parameters perfomu:d average to below average in detection of gear tooth surface pitting failures. The ODM sensor was successful in detecting a significant 8lDOunt of debris from all the gear tooth pitting fatigue failures. Excluding outliers, the average cumulative mass at the end of a test was 40 mg.

  10. Re-examination of cumulative fatigue damage analysis: An engineering perspective

    NASA Technical Reports Server (NTRS)

    Manson, S. S.; Halford, G. R.

    1986-01-01

    A method which has evolved in our laboratories for the past 20 yr is re-examined with the intent of improving its accuracy and simplicity of application to engineering problems. Several modifications are introduced both to the analytical formulation of the Damage Curve Approach, and to the procedure for modifying this approach to achieve a Double Linear Damage Rule formulation which immensely simplifies the calculation. Improvements are also introduced in the treatment of mean stress for determining fatigue life of the individual events that enter into a complex loading history. While the procedure is completely consistent with the results of numerous two level tests that have been conducted on many materials, it is still necessary to verify applicability to complex loading histories. Caution is expressed that certain phenomena can also influence the applicability - for example, unusual deformation and fracture modes inherent in complex loading - especially if stresses are multiaxial. Residual stresses at crack tips, and metallurgical factors are also important in creating departures from the cumulative damage theories; examples of departures are provided.

  11. Re-examination of cumulative fatigue damage analysis - An engineering perspective

    NASA Technical Reports Server (NTRS)

    Manson, S. S.; Halford, G. R.

    1986-01-01

    A method which has evolved in the laboratories for the past 20 yr is re-examined with the intent of improving its accuracy and simplicity of application to engineering problems. Several modifications are introduced both to the analytical formulation of the Damage Curve Approach, and to the procedure for modifying this approach to achieve a Double Linear Damage Rule formulation which immensely simplifies the calculation. Improvements are also introduced in the treatment of mean stress for determining fatigue life of the individual events that enter into a complex loading history. While the procedure is completely consistent with the results of numerous two level tests that have been conducted on many materials, it is still necessary to verify applicability to complex loading histories. Caution is expressed that certain phenomenon can also influence the applicability - for example, unusual deformation and fracture modes inherent in complex loading especially if stresses are multiaxial. Residual stresses at crack tips, and metallurgical factors are also important in creating departures from the cumulative damage theories; examples of departures are provided.

  12. Optical sensing of the fatigue damage state of CFRP under realistic aeronautical load sequences.

    PubMed

    Zuluaga-Ramírez, Pablo; Arconada, Álvaro; Frövel, Malte; Belenguer, Tomás; Salazar, Félix

    2015-03-09

    We present an optical sensing methodology to estimate the fatigue damage state of structures made of carbon fiber reinforced polymer (CFRP), by measuring variations on the surface roughness. Variable amplitude loads (VAL), which represent realistic loads during aeronautical missions of fighter aircraft (FALSTAFF) have been applied to coupons until failure. Stiffness degradation and surface roughness variations have been measured during the life of the coupons obtaining a Pearson correlation of 0.75 between both variables. The data were compared with a previous study for Constant Amplitude Load (CAL) obtaining similar results. Conclusions suggest that the surface roughness measured in strategic zones is a useful technique for structural health monitoring of CFRP structures, and that it is independent of the type of load applied. Surface roughness can be measured in the field by optical techniques such as speckle, confocal perfilometers and interferometry, among others.

  13. Bone maintenance and remodeling: a control system based on fatigue damage.

    PubMed

    Taylor, D

    1997-07-01

    This paper explores the relationship between damage, repair, and remodeling in compact bone. A model of microcrack growth is developed that takes account of recent findings on the behaviour of small fatigue cracks in other materials. This is combined with a simple model of a repair process, envisaged as a constant rate of decrease in crack length. The system that results is capable of achieving a stable and precise control of crack length without the need to measure it. This is very useful because it implies that bone does not require the complexities of crack-measuring transducers or active decision-making processes. A simple explanation is suggested for the presence of a "lazy zone" of remodeling equilibrium strains, and the limits of this zone are quantified. The model is developed through a necessarily simplified geometry and loading scheme but can be extended to provide a general solution applicable to in vivo conditions.

  14. Optical sensing of the fatigue damage state of CFRP under realistic aeronautical load sequences.

    PubMed

    Zuluaga-Ramírez, Pablo; Arconada, Álvaro; Frövel, Malte; Belenguer, Tomás; Salazar, Félix

    2015-01-01

    We present an optical sensing methodology to estimate the fatigue damage state of structures made of carbon fiber reinforced polymer (CFRP), by measuring variations on the surface roughness. Variable amplitude loads (VAL), which represent realistic loads during aeronautical missions of fighter aircraft (FALSTAFF) have been applied to coupons until failure. Stiffness degradation and surface roughness variations have been measured during the life of the coupons obtaining a Pearson correlation of 0.75 between both variables. The data were compared with a previous study for Constant Amplitude Load (CAL) obtaining similar results. Conclusions suggest that the surface roughness measured in strategic zones is a useful technique for structural health monitoring of CFRP structures, and that it is independent of the type of load applied. Surface roughness can be measured in the field by optical techniques such as speckle, confocal perfilometers and interferometry, among others. PMID:25760056

  15. Optical Sensing of the Fatigue Damage State of CFRP under Realistic Aeronautical Load Sequences

    PubMed Central

    Zuluaga-Ramírez, Pablo; Arconada, Álvaro; Frövel, Malte; Belenguer, Tomás; Salazar, Félix

    2015-01-01

    We present an optical sensing methodology to estimate the fatigue damage state of structures made of carbon fiber reinforced polymer (CFRP), by measuring variations on the surface roughness. Variable amplitude loads (VAL), which represent realistic loads during aeronautical missions of fighter aircraft (FALSTAFF) have been applied to coupons until failure. Stiffness degradation and surface roughness variations have been measured during the life of the coupons obtaining a Pearson correlation of 0.75 between both variables. The data were compared with a previous study for Constant Amplitude Load (CAL) obtaining similar results. Conclusions suggest that the surface roughness measured in strategic zones is a useful technique for structural health monitoring of CFRP structures, and that it is independent of the type of load applied. Surface roughness can be measured in the field by optical techniques such as speckle, confocal perfilometers and interferometry, among others. PMID:25760056

  16. Multidisciplinary design optimization of a fighter aircraft with damage tolerance constraints and a probabilistic model of the fatigue environment

    NASA Astrophysics Data System (ADS)

    Arrieta, Albert Joseph

    2001-07-01

    Damage tolerance analysis (DTA) was considered in the global design optimization of an aircraft wing structure. Residual strength and fatigue life requirements, based on the damage tolerance philosophy, were investigated as new design constraints. In general, accurate fatigue prediction is difficult if the load environment is not known with a high degree of certainty. To address this issue, a probabilistic approach was used to describe the uncertain load environment. Probabilistic load spectra models were developed from flight recorder data. The global/local finite element approach allowed local fatigue requirements to be considered in the global design optimization. AFGROW fatigue crack growth analysis provided a new strength criterion for satisfying damage tolerance requirements within a global optimization environment. Initial research with the ASTROS program used the probabilistic load model and this damage tolerance constraint to optimize cracked skin panels on the lower wing of a fighter/attack aircraft. For an aerodynamic and structural model similar to an F-16, ASTROS simulated symmetric and asymmetric maneuvers during the optimization. Symmetric maneuvers, without underwing stores, produced the highest stresses and drove the optimization of the inboard lower wing skin. Asymmetric maneuvers, with underwing stores, affected the optimum thickness of the outboard hard points. Subsequent design optimizations included von Mises stress, aileron effectiveness, and lift effectiveness constraints simultaneously. This optimization was driven by the DTA and von Mises stress constraints and, therefore, DTA requirements can have an active role to play in preliminary aircraft design.

  17. Modeling Fatigue Damage Onset and Progression in Composites Using an Element-Based Virtual Crack Closure Technique Combined With the Floating Node Method

    NASA Technical Reports Server (NTRS)

    De Carvalho, Nelson V.; Krueger, Ronald

    2016-01-01

    A new methodology is proposed to model the onset and propagation of matrix cracks and delaminations in carbon-epoxy composites subject to fatigue loading. An extended interface element, based on the Floating Node Method, is developed to represent delaminations and matrix cracks explicitly in a mesh independent fashion. Crack propagation is determined using an element-based Virtual Crack Closure Technique approach to determine mixed-mode energy release rates, and the Paris-Law relationship to obtain crack growth rate. Crack onset is determined using a stressbased onset criterion coupled with a stress vs. cycle curve and Palmgren-Miner rule to account for fatigue damage accumulation. The approach is implemented in Abaqus/Standard® via the user subroutine functionality. Verification exercises are performed to assess the accuracy and correct implementation of the approach. Finally, it was demonstrated that this approach captured the differences in failure morphology in fatigue for two laminates of identical stiffness, but with layups containing ?deg plies that were either stacked in a single group, or distributed through the laminate thickness.

  18. Effects of multiaxial stress state and saltwater on fatigue damage and failure of glass/epoxy composite

    NASA Astrophysics Data System (ADS)

    Wang, Feng

    2007-12-01

    Fiber composites used in advanced mechanical systems are generally subjected to cyclic loading with multiaxial stress state. Thus multiaxial fatigue is a subject of major importance in design and evaluation of long-term performance of composite structures and components. Theoretical work was initially performed to address the fundamental issues of damage evolution and material degradation in glass/epoxy composites subject to multiaxial cyclic stresses. A continuum damage mechanics formulation based on irreversible thermodynamics was used to construct multiaxial cyclic constitutive equations of the composite. With coupling of damage anisotropy and multiaxial stress state and using Gibb's free energy function, damage driving force was determined. Based on the second principle of thermodynamics, evolution equations were also obtained to describe the change of the damage state in the composite under multiaxial fatigue loading. A newly introduced failure-life theory was employed for the fiber composite, based on anisotropic nature of composite strength property and distinct mechanisms of microcrack formation and growth under different multiaxial cyclic stresses. A driving force for fatigue failure was formulated with a governing function, involving combined cyclic shear and transverse tensile stresses along the weakest principal material plane of the composite. Multiaxial fatigue life of the composite was related to the forcing function using a power-law. A formulation of thermomechanical response of a fiber composite based on the general thermodynamics theory was proposed to establish a model that can couple water sorption, applied stress and damage in the composite. Starting from general Gibbs free energy, constitutive equations, which describe the relationships between state variables and corresponding driving forces, were derived. The evolution laws that describe relationships of damage driving force-damage evolution and chemical potential gradient-water flux were

  19. Simulation of long-term fatigue damage in bioprosthetic heart valves: effects of leaflet and stent elastic properties

    PubMed Central

    Martin, Caitlin

    2014-01-01

    One of the major failure modes of bioprosthetic heart valves (BHVs) is noncalcific structural deterioration due to fatigue of the tissue leaflets; yet, the mechanisms of fatigue are not well understood. BHV durability is primarily assessed based on visual inspection of the leaflets following accelerated wear testing. In this study, we developed a computational framework to simulate BHV leaflet fatigue, which is both efficient and quantitative, making it an attractive alternative to traditional accelerated wear testing. We utilize a phenomenological soft tissue fatigue damage model developed previously to describe the stress softening and permanent set of the glutaraldehyde-treated bovine pericardium leaflets in BHVs subjected to cyclic loading. A parametric study was conducted to determine the effects of altered leaflet and stent elastic properties on the fatigue of the leaflets. The simulation results show that heterogeneity of the leaflet elastic properties, poor leaflet coaptation, and little stent-tip deflection may accelerate leaflet fatigue, which agrees with clinical findings. Therefore, the developed framework may be an invaluable tool for evaluating leaflet durability in new tissue valve designs, including traditional BHVs as well as new transcatheter valves. PMID:24092257

  20. Prediction of damage evolution in continuous fiber metal matrix composites subjected to fatigue loading

    SciTech Connect

    Allen, D.; Helms, K.; Lagoudas, D.

    1995-08-01

    A life prediction model is being developed by the authors for application to metal matrix composites (MMC`s). The systems under study are continuous silicon carbide fibers imbedded in titanium matrix. The model utilizes a computationally based framework based on thermodynamics and continuum mechanics, and accounts for matrix inelasticity, damage evolution, and environmental degradation due to oxidation. The computational model utilizes the finite element method, and an evolutionary analysis of a unit cell is accomplished via a time stepping algorithm. The computational scheme accounts for damage growth such as fiber-matrix debonding, surface cracking, and matrix cracking via the inclusion of cohesive zone elements in the unit cell. These elements are located based on experimental evidence also obtained by the authors. The current paper outlines the formulation utilized by the authors to solve this problem, and recent results are discussed. Specifically, results are given for a four-ply unidirectional composite subjected to cyclic fatigue loading at 650{degrees}C both in air and inert gas. The effects of oxidation on the life of the composite are predicted with the model, and the results are compared to limited experimental results.

  1. Cross-flow VIV-induced fatigue damage of deepwater steel catenary riser at touch-down point

    NASA Astrophysics Data System (ADS)

    Wang, Kun-peng; Tang, Wen-yong; Xue, Hong-xiang

    2014-03-01

    A prediction model of the deepwater steel catenary riser VIV is proposed based on the forced oscillation test data, taking into account the riser-seafloor interaction for the cross-flow VIV-induced fatigue damage at touch-down point (TDP). The model will give more reasonable simulation of SCR response near TDP than the previous pinned truncation model. In the present model, the hysteretic riser-soil interaction model is simplified as the linear spring and damper to simulate the seafloor, and the damping is obtained according to the dissipative power during one periodic riser-soil interaction. In order to validate the model, the comparison with the field measurement and the results predicted by Shear 7 program of a full-scale steel catenary riser is carried out. The main induced modes, mode frequencies and response amplitude are in a good agreement. Furthermore, the parametric studies are carried out to broaden the understanding of the fatigue damage sensitivity to the upper end in-plane offset and seabed characteristics. In addition, the fatigue stress comparison at TDP between the truncation riser model and the present full riser model shows that the existence of touch-down zones is very important for the fatigue damage assessment of steel catenary riser at TDP.

  2. DNA repair decline during mouse spermiogenesis results in the accumulation of heritable DNA damage

    SciTech Connect

    Marchetti, Francesco; Marchetti, Francesco; Wryobek, Andrew J

    2008-02-21

    The post-meiotic phase of mouse spermatogenesis (spermiogenesis) is very sensitive to the genomic effects of environmental mutagens because as male germ cells form mature sperm they progressively lose the ability to repair DNA damage. We hypothesized that repeated exposures to mutagens during this repair-deficient phase result in the accumulation of heritable genomic damage in mouse sperm that leads to chromosomal aberrations in zygotes after fertilization. We used a combination of single or fractionated exposures to diepoxybutane (DEB), a component of tobacco smoke, to investigate how differential DNA repair efficiencies during the three weeks of spermiogenesis affected the accumulation of DEB-induced heritable damage in early spermatids (21-15 days before fertilization, dbf), late spermatids (14-8 dbf) and sperm (7- 1 dbf). Analysis of chromosomalaberrations in zygotic metaphases using PAINT/DAPI showed that late spermatids and sperm are unable to repair DEB-induced DNA damage as demonstrated by significant increases (P<0.001) in the frequencies of zygotes with chromosomal aberrations. Comparisons between single and fractionated exposures suggested that the DNA repair-deficient window during late spermiogenesis may be less than two weeks in the mouse and that during this repair-deficient window there is accumulation of DNA damage in sperm. Finally, the dose-response study in sperm indicated a linear response for both single and repeated exposures. These findings show that the differential DNA repair capacity of post-meioitic male germ cells has a major impact on the risk of paternally transmitted heritable damage and suggest that chronic exposures that may occur in the weeks prior to fertilization because of occupational or lifestyle factors (i.e, smoking) can lead to an accumulation of genetic damage in sperm and result in heritable chromosomal aberrations of paternal origin.

  3. DNA Repair Decline During Mouse Spermiogenesis Results in the Accumulation of Heritable DNA Damage

    SciTech Connect

    Marchetti, Francesco; Marchetti, Francesco; Wyrobek, Andrew J.

    2007-12-01

    The post-meiotic phase of mouse spermatogenesis (spermiogenesis) is very sensitive to the genomic effects of environmental mutagens because as male germ cells form mature sperm they progressively lose the ability to repair DNA damage. We hypothesized that repeated exposures to mutagens during this repair-deficient phase result in the accumulation of heritable genomic damage in mouse sperm that leads to chromosomal aberrations in zygotes after fertilization. We used a combination of single or fractionated exposures to diepoxybutane (DEB), a component of tobacco smoke, to investigate how differential DNA repair efficiencies during the three weeks of spermiogenesis affected the accumulation of DEB-induced heritable damage in early spermatids (21-15 days before fertilization, dbf), late spermatids (14-8 dbf) and sperm (7-1 dbf). Analysis of chromosomal aberrations in zygotic metaphases using PAINT/DAPI showed that late spermatids and sperm are unable to repair DEB-induced DNA damage as demonstrated by significant increases (P<0.001) in the frequencies of zygotes with chromosomal aberrations. Comparisons between single and fractionated exposures suggested that the DNA repair-deficient window during late spermiogenesis may be less than two weeks in the mouse and that during this repair-deficient window there is accumulation of DNA damage in sperm. Finally, the dose-response study in sperm indicated a linear response for both single and repeated exposures. These findings show that the differential DNA repair capacity of post-meioitic male germ cells has a major impact on the risk of paternally transmitted heritable damage and suggest that chronic exposures that may occur in the weeks prior to fertilization because of occupational or lifestyle factors (i.e, smoking) can lead to an accumulation of genetic damage in sperm and result in heritable chromosomal aberrations of paternal origin.

  4. Damage production and accumulation in SiC structures in inertial and magnetic fusion systems

    NASA Astrophysics Data System (ADS)

    Sawan, M. E.; Ghoniem, N. M.; Snead, L.; Katoh, Y.

    2011-10-01

    Radiation damage parameters in SiC/SiC composite structures are determined in both magnetic (MFE) and inertial (IFE) confinement fusion systems. Variations in the geometry, neutron energy spectrum, and pulsed nature of neutron production result in significant differences in damage parameters between the two systems. With the same neutron wall loading, the displacement damage rate in the first wall in an IFE system is ˜10% lower than in an MFE system, while gas production and burnup rates are a factor of 2 lower. Self-cooled LiPb and Flibe blankets were analyzed. While using LiPb results in higher displacement damage, Flibe yields higher gas production and burnup rates. The effects of displacement damage and helium production on defect accumulation in SiC/SiC composites are also discussed.

  5. Damage accumulation in ion-irradiated Ni-based concentrated solid-solution alloys

    DOE PAGES

    Ullah, Mohammad W.; Aidhy, Dilpuneet S.; Zhang, Yanwen; Weber, William J.

    2016-01-01

    We investigate Irradiation-induced damage accumulation in Ni0.8Fe0.2 and Ni0.8Cr0.2 alloys by using molecular dynamics simulations to assess possible enhanced radiation-resistance in these face-centered cubic (fcc), single-phase, concentrated solid-solution alloys, as compared with pure fcc Ni.

  6. The mitochondrial theory of aging: do damaged mitochondria accumulate by delayed degradation?

    PubMed

    Kowald, A

    1999-08-01

    The mitochondrial theory of aging states that the slow accumulation of impaired mitochondria is the driving force of the aging process. In recent years, this theory has gained new support with the discovery of age-related mitochondrial DNA deletions. However, the underlying mechanism of the accumulation of defective mitochondria remained unclear. This has changed recently with the proposal of de Grey that damaged mitochondria have a decreased degradation rate. The resulting increase in biological half-life would be a strong selection advantage leading to the accumulation of defective mitochondria. In this article, I summarize current ideas on how damaged organelles can build up in a cell as well as the shortcomings of these ideas. Then the new hypothesis and its justification are described. It appears that de Grey's hypothesis is a very promising concept that elegantly solves inconsistencies of current models and is in accordance with experimental findings. PMID:10530786

  7. Effects of successive judo matches on fatigue and muscle damage markers.

    PubMed

    Detanico, Daniele; Dal Pupo, Juliano; Franchini, Emerson; Dos Santos, Saray G

    2015-04-01

    This study aimed to investigate the acute effects of simulated judo matches on fatigue and muscle damage markers. Twenty male judo athletes participated in this study. The athletes performed three 5-minute judo matches separated by 15 minutes of passive rest between each match. The following measurements were performed before and after each match: shoulder external/internal rotation isokinetic torque and countermovement jump (CMJ). Blood samples were taken before the first match and after the third match for serum creatine kinase (CK) and lactate dehydrogenase (LDH) analysis. T-tests for dependent samples and analysis of variance for repeated measures were used to compare the variables over the time; the level of significance was set at 0.05. An overall effect of the successive matches on shoulder internal (PTIN) and external (PTEX) rotation peak torque and CMJ performance was observed. PTIN and PTEX showed significant decreases in postmatch 2 and postmatch 3 when compared with the baseline (p < 0.01). Also, CMJ height declined in postmatch 2 and postmatch 3 (p < 0.01) when compared with the baseline. Serum CK and LDH activity increased significantly after the third match (p < 0.01). It was concluded that 3 successive judo matches induced a decline of peak torque and muscle power in the upper and lower limbs, respectively, and also provoked an increase of muscle damage markers. These findings may provide important knowledge for coaches and physical trainers to improve judo-specific strength training in both the upper and lower limbs. PMID:25426512

  8. Damage development under compression-compression fatigue loading in a stitched uniwoven graphite/epoxy composite material

    NASA Technical Reports Server (NTRS)

    Vandermey, Nancy E.; Morris, Don H.; Masters, John E.

    1991-01-01

    Damage initiation and growth under compression-compression fatigue loading were investigated for a stitched uniweave material system with an underlying AS4/3501-6 quasi-isotropic layup. Performance of unnotched specimens having stitch rows at either 0 degree or 90 degrees to the loading direction was compared. Special attention was given to the effects of stitching related manufacturing defects. Damage evaluation techniques included edge replication, stiffness monitoring, x-ray radiography, residual compressive strength, and laminate sectioning. It was found that the manufacturing defect of inclined stitches had the greatest adverse effect on material performance. Zero degree and 90 degree specimen performances were generally the same. While the stitches were the source of damage initiation, they also slowed damage propagation both along the length and across the width and affected through-the-thickness damage growth. A pinched layer zone formed by the stitches particularly affected damage initiation and growth. The compressive failure mode was transverse shear for all specimens, both in static compression and fatigue cycling effects.

  9. Characterization of failure processes in tungsten copper composites under fatigue loading conditions

    NASA Technical Reports Server (NTRS)

    Kim, Yong-Suk; Verrilli, Michael J.; Gabb, Timothy P.

    1989-01-01

    A fractographic and metallographic investigation was performed on specimens of a tungsten fiber reinforced copper matrix composite (9 vol percent), which had experienced fatigue failures at elevated temperatures. Major failure modes and possible failure mechanisms, with an emphasis placed on characterizing fatigue damage accumulation, were determined. Metallography of specimens fatigued under isothermal cyclic loading suggested that fatigue damage initiates in the matrix. Cracks nucleated within the copper matrix at grain boundaries, and they propagated through cavity coalescence. The growing cracks subsequently interacted with the reinforcing tungsten fibers, producing a localized ductile fiber failure. Examinations of interrupted tests before final failure confirmed the suggested fatigue damage processes.

  10. Use of Acoustic Emission to Monitor Progressive Damage Accumulation in Kevlar (R) 49 Composites

    NASA Technical Reports Server (NTRS)

    Waller, Jess M.; Saulsberry, Regor L.; Andrade, Eduardo

    2009-01-01

    Acoustic emission (AE) data acquired during intermittent load hold tensile testing of epoxy impregnated Kevlar(Registeres TradeMark) 49 (K/Ep) composite strands were analyzed to monitor progressive damage during the approach to tensile failure. Insight into the progressive damage of K/Ep strands was gained by monitoring AE event rate and energy. Source location based on energy attenuation and arrival time data was used to discern between significant AE attributable to microstructural damage and spurious AE attributable to noise. One of the significant findings was the observation of increasing violation of the Kaiser effect (Felicity ratio < 1.0) with damage accumulation. The efficacy of three different intermittent load hold stress schedules that allowed the Felicity ratio to be determined analytically is discussed.

  11. Stochastic propagation of an array of parallel cracks: Exploratory work on matrix fatigue damage in composite laminates

    SciTech Connect

    Williford, R.E.

    1989-09-01

    Transverse cracking of polymeric matrix materials is an important fatigue damage mechanism in continuous-fiber composite laminates. The propagation of an array of these cracks is a stochastic problem usually treated by Monte Carlo methods. However, this exploratory work proposes an alternative approach wherein the Monte Carlo method is replaced by a more closed-form recursion relation based on fractional Brownian motion.'' A fractal scaling equation is also proposed as a substitute for the more empirical Paris equation describing individual crack growth in this approach. Preliminary calculations indicate that the new recursion relation is capable of reproducing the primary features of transverse matrix fatigue cracking behavior. Although not yet fully tested or verified, this cursion relation may eventually be useful for real-time applications such as monitoring damage in aircraft structures.

  12. Combining passive thermography and acoustic emission for large area fatigue damage growth assessment of a composite structure

    NASA Astrophysics Data System (ADS)

    Zalameda, Joseph N.; Horne, Michael R.; Madaras, Eric I.; Burke, Eric R.

    2016-05-01

    Passive thermography and acoustic emission data were obtained for improved real time damage detection during fatigue loading. A strong positive correlation was demonstrated between acoustic energy event location and thermal heating, especially if the structure under load was nearing ultimate failure. An image processing routine was developed to map the acoustic emission data onto the thermal imagery. This required removing optical barrel distortion and angular rotation from the thermal data. The acoustic emission data were then mapped onto thermal data, revealing the cluster of acoustic emission event locations around the thermal signatures of interest. By combining both techniques, progression of damage growth is confirmed and areas of failure are identified. This technology provides improved real time inspections of advanced composite structures during fatigue testing.

  13. A thermodynamics based damage mechanics framework for fatigue analysis of microelectronics solder joints with size effects

    NASA Astrophysics Data System (ADS)

    Gomez, Juan

    Experimental observations of an increase in resistance with decreasing specimen size and under the presence of non-uniform plastic deformation fields have pushed the development for small scale plasticity theories since the early 90's. The observed phenomenon has been explained in terms of an accumulation of a density of geometrically necessary dislocations, which is required in order to accommodate nonuniform plastic deformation fields. This extra density of dislocations, contributes to the additional hardening observed in small scale specimens under imposed non-uniform plastic deformations. The density of geometrically necessary dislocations has been related to the gradients of plastic strain which are imposed either by the loading conditions or the geometry of the specimen. The proposed set of theories has promoted the idea that there is an additional material parameter, namely a plastic length scale. Within these theories when the material is under the presence of a non-uniform plastic deformation field and once typical structural dimensions approaches the material length scale there is an increase in resistance. Such a class of mathematical framework is currently known as strain gradient plasticity (SGP) theory. On the other hand, the current trend towards miniaturization in the microelectronics industry has raised questions about the true behavior of small structural systems. In this dissertation we address such a problem but from the perspective of eutectic solder alloys. Eutectic solder alloys as frequently used in the microelectronics industry exhibit considerable rate dependent response even at room temperature. Moreover for this type of material, the problem of interest is the response under cyclic loadings induced by thermomechanical fatigue leading to the classical case of creep-fatigue interaction. Several experimental and theoretical studies have been developed in order to generate robust constitutive descriptions for this class of applications. For

  14. Damage Accumulation in Cyclically-Loaded Glass-Ceramic Matrix Composites Monitored by Acoustic Emission

    PubMed Central

    Aggelis, D. G.; Dassios, K. G.; Kordatos, E. Z.; Matikas, T. E.

    2013-01-01

    Barium osumilite (BMAS) ceramic matrix composites reinforced with SiC-Tyranno fibers are tested in a cyclic loading protocol. Broadband acoustic emission (AE) sensors are used for monitoring the occurrence of different possible damage mechanisms. Improved use of AE indices is proposed by excluding low-severity signals based on waveform parameters, rather than only threshold criteria. The application of such improvements enhances the accuracy of the indices as accumulated damage descriptors. RA-value, duration, and signal energy follow the extension cycles indicating moments of maximum or minimum strain, while the frequency content of the AE signals proves very sensitive to the pull-out mechanism. PMID:24381524

  15. Redox instability, mechanical deformation, and heterogeneous damage accumulation in solid oxide fuel cell anodes

    NASA Astrophysics Data System (ADS)

    Abdeljawad, F.; Nelson, G. J.; Chiu, W. K. S.; Haataja, M.

    2012-08-01

    Mechanical integrity and damage tolerance represent two key challenges in the design of solid oxide fuel cells (SOFCs). In particular, reduction and oxidation (redox) cycles, and the associated large transformation strains have a notable impact on the mechanical stability and failure mode of SOFC anodes. In this study, the deformation behavior under redox cycling is investigated computationally with an approach that provides a detailed, microstructurally based view of heterogeneous damage accumulation behavior within an experimentally obtained nickel/yttria stabilized zirconia SOFC anode microstructure. Simulation results underscore the critical role that the microstructure plays in the mechanical deformation behavior of and failure within such materials.

  16. Atomistic modeling of nanowires, small-scale fatigue damage in cast magnesium, and materials for MEMS

    SciTech Connect

    Dunn, Martin L.; Talmage, Mellisa J.; David L. McDowell; West, Neil; Gullett, Philip Michael; Miller, David C.; Spark, Kevin; Diao, Jiankuai; Horstemeyer, Mark F.; Zimmerman, Jonathan A.; Gall, K.

    2006-10-01

    titled 'Atomistic Modeling of Nanowires, Small-scale Fatigue Damage in Cast Magnesium, and Materials for MEMS'. This project supported a strategic partnership between Sandia National Laboratories and the University of Colorado at Boulder by providing funding for the lead author, Ken Gall, and his students, while he was a member of the University of Colorado faculty.

  17. The effect of helium accumulation and radiation damage on the weldability of 316-type steel

    NASA Astrophysics Data System (ADS)

    Fabritsiev, S. A.; Pokrovsky, A. S.

    1998-10-01

    The embrittlement simulated by helium accumulation, with the ITER components repaired by welding during maintenance, is one of the factors limiting the materials lifetime. This work presents the results of the investigations into the effect of neutron produced helium, 0.1 dpa Tirr - 80°C, (at a low He/dpa and cyclotron introduced helium, 5-430 appm He (at high He/dpa) on the quality and mechanical properties of the type 316 steel welds. The samples, both irradiated and controls, were welded by e-beam welding (cyclotron injection) or on the automatic argon-arc welding device in the hot cell SRIIAr (neutron irradiation). Low-cycle fatigue (LCF) testing in bending was used to assess impact of helium on the degradation of welded joint properties.

  18. APPLICABILITY OF A ACCUMULATED DAMAGE PARAMETER METHOD ON SOIL LIQUEFACTION DUE TOSEVERAL EARTHQUAKES

    NASA Astrophysics Data System (ADS)

    Izawa, Jun; Tanoue, Kazuya; Murono, Yoshitaka

    Severe soil liquefaction due to long duration earthquake with low acceleration occurred at Tokyo Bay area in the 2011 off the Pacific coast of Tohoku Earthquake. This phenomenon clearly shows that soil liquefaction is affected by properties of input waves. This paper describes effect of wave properties of earthquake on liquefaction using Effective Stress analysis with some earthquakes. Analytical result showedthat almost the same pore water pressure was observed due to both long durationearthquake with max acceleration of 150Gal and typical inland active fault earthquake with 891Gal. Additionally, lique-faction potentials for each earthquake were evaluated by simple judgment with accumulated damage parameter, which is used for design of railway structuresin Japan. As a result, it was found that accurate liquefaction resistance on large cyclic area is necessaryto evaluate liquefaction potential due to long duration earthquake with low acceleration with simple judgment with accumulated damage parameter.

  19. Damage accumulation in ion-irradiated Ni-based concentrated solid-solution alloys

    SciTech Connect

    Ullah, Mohammad W.; Aidhy, Dilpuneet S.; Zhang, Yanwen; Weber, William J.

    2016-01-01

    We investigate Irradiation-induced damage accumulation in Ni0.8Fe0.2 and Ni0.8Cr0.2 alloys by using molecular dynamics simulations to assess possible enhanced radiation-resistance in these face-centered cubic (fcc), single-phase, concentrated solid-solution alloys, as compared with pure fcc Ni.

  20. DNA-damage accumulation and replicative arrest in Hutchinson-Gilford progeria syndrome.

    PubMed

    Musich, Phillip R; Zou, Yue

    2011-12-01

    A common feature of progeria syndromes is a premature aging phenotype and an enhanced accumulation of DNA damage arising from a compromised repair system. HGPS (Hutchinson-Gilford progeria syndrome) is a severe form of progeria in which patients accumulate progerin, a mutant lamin A protein derived from a splicing variant of the lamin A/C gene (LMNA). Progerin causes chromatin perturbations which result in the formation of DSBs (double-strand breaks) and abnormal DDR (DNA-damage response). In the present article, we review recent findings which resolve some mechanistic details of how progerin may disrupt DDR pathways in HGPS cells. We propose that progerin accumulation results in disruption of functions of some replication and repair factors, causing the mislocalization of XPA (xeroderma pigmentosum group A) protein to the replication forks, replication fork stalling and, subsequently, DNA DSBs. The binding of XPA to the stalled forks excludes normal binding by repair proteins, leading to DSB accumulation, which activates ATM (ataxia telangiectasia mutated) and ATR (ATM- and Rad3-related) checkpoints, and arresting cell-cycle progression.

  1. Creep-fatigue damage evaluation of a nickel-base heat-resistant alloy Hastelloy XR in simulated HTGR helium gas environment

    NASA Astrophysics Data System (ADS)

    Tsuji, Hirokazu; Nakajima, Hajime

    1994-02-01

    The properties of Hastelloy XR, which is a developed alloy as the structural material for high-temperature components of the HTTR, under creep-fatigue interaction conditions were examined by performing a series of axial strain-controlled fully reversed fatigue tests in the simulated HTGR helium gas environment at 700, 800, 900 and 950°C. Two types of evaluation techniques, i.e., the life fraction rule and the ductility exhaustion one, were applied for the evaluation of the creep damage during the strain holding. The fatigue life reduction due to the strain holding is observed even at hold times of 6 s, and the saturation point of the fatigue life reduction shifts to the shorter hold time side with increasing temperature. The life fraction rule predicts an excessively conservative value for the creep damage. The ductility exhaustion rule can predict the fatigue life under the effective creep condition much more successfully than the life fraction one.

  2. Targeting the age-related occurrence, removal, and accumulation of molecular damage by hormesis.

    PubMed

    Rattan, Suresh I S

    2010-06-01

    Strategies for testing and developing effective means of intervention, prevention, and modulation of aging incorporate means to minimize the occurrence and accumulation of molecular damage, to reduce molecular heterogeneity, and to evaluate the relevance of the type and extent of damage with respect to its role in aging and age-related diseases. One such approach is that of mild stress-induced hormesis, which stimulates maintenance and repair systems and strengthens the homeodynamic space of cells and organisms. Hormesis through mild heat shock, natural and synthetic hormetins, and other stressors brings about several antiaging effects in human fibroblasts, keratinocytes, and telomerase-immortalized bone marrow stem cells. Depending on the cell type, these antiaging hormetic effects include extension of replicative life span, enhanced proteasomal activities, increased chaperone levels, and improved wound healing, angiogenesis, and differentiation. The main molecular pathways for achieving such hormetic effects are through targeting the processes for the repair and removal of molecular damage, which can slow aging.

  3. Environmental fatigue of an Al-Li-Cu alloy. Part 3: Modeling of crack tip hydrogen damage

    NASA Technical Reports Server (NTRS)

    Piascik, Robert S.; Gangloff, Richard P.

    1992-01-01

    Environmental fatigue crack propagation rates and microscopic damage modes in Al-Li-Cu alloy 2090 (Parts 1 and 2) are described by a crack tip process zone model based on hydrogen embrittlement. Da/dN sub ENV equates to discontinuous crack advance over a distance, delta a, determined by dislocation transport of dissolved hydrogen at plastic strains above a critical value; and to the number of load cycles, delta N, required to hydrogenate process zone trap sites that fracture according to a local hydrogen concentration-tensile stress criterion. Transgranular (100) cracking occurs for process zones smaller than the subgrain size, and due to lattice decohesion or hydride formation. Intersubgranular cracking dominates when the process zone encompasses one or more subgrains so that dislocation transport provides hydrogen to strong boundary trapping sites. Multi-sloped log da/dN-log delta K behavior is produced by process zone plastic strain-hydrogen-microstructure interactions, and is determined by the DK dependent rates and proportions of each parallel cracking mode. Absolute values of the exponents and the preexponential coefficients are not predictable; however, fractographic measurements theta sub i coupled with fatigue crack propagation data for alloy 2090 established that the process zone model correctly describes fatigue crack propagation kinetics. Crack surface films hinder hydrogen uptake and reduce da/dN and alter the proportions of each fatigue crack propagation mode.

  4. Transgenerational accumulation of radiation damage in small mammals chronically exposed to Chernobyl fallout.

    PubMed

    Ryabokon, Nadezhda I; Goncharova, R I

    2006-09-01

    The purpose of this investigation has been the analysis of the long-term development of biological damage in natural populations of a model mammalian species, the bank vole (Clethrionomys glareolus, Schreber), which were chronically exposed to low doses of ionizing radiation over 22 animal generations within 10 years following the Chernobyl accident. The time course of the biological end-points (chromosome aberrations in bone marrow cells and embryonic lethality) was compared with the time course of the whole-body absorbed dose rate from external and internal exposure in the studied populations inhabiting monitoring sites in Belarus with different ground deposition of radionuclides. The yield of chromosome aberrations and, in lesser degree, embryonic lethality was associated with the radionuclide contamination of the monitoring areas in a dose-dependent manner. As a main feature of the long-term development of biological damage under low dose rate irradiation, permanently elevated levels of chromosome aberrations and an increasing frequency of embryonic lethality have developed over 22 animal generations. This contrasts with the assumption that the biological damage would gradually disappear since in the same period of time the whole-body absorbed dose rate decreased exponentially with a half-value time of about 2.5-3 years. Furthermore, gravid females were captured, and their offspring, born and grown up under contamination-free laboratory conditions, showed the same enhanced level of chromosome aberrations. Therefore the authors suggest that, along with the biological damage attributable to the individual exposure of each animal, the observed cellular and systemic effects reflect the transgenerational transmission and accumulation, via genetic and/or epigenetic pathways, of damage attributable to the chronic low-dose rate exposure of the preceding generations of animals. They also suggest that the level of the accumulated transmissible damage in the investigated

  5. Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage.

    PubMed

    Cousin, Wendy; Ho, Michelle Liane; Desai, Rajiv; Tham, Andrea; Chen, Robert Yuzen; Kung, Sunny; Elabd, Christian; Conboy, Irina M

    2013-01-01

    The performance of adult stem cells is crucial for tissue homeostasis but their regenerative capacity declines with age, leading to failure of multiple organs. In skeletal muscle this failure is manifested by the loss of functional tissue, the accumulation of fibrosis, and reduced satellite cell-mediated myogenesis in response to injury. While recent studies have shown that changes in the composition of the satellite cell niche are at least in part responsible for the impaired function observed with aging, little is known about the effects of aging on the intrinsic properties of satellite cells. For instance, their ability to repair DNA damage and the effects of a potential accumulation of DNA double strand breaks (DSBs) on their regenerative performance remain unclear. This work demonstrates that old muscle stem cells display no significant accumulation of DNA DSBs when compared to those of young, as assayed after cell isolation and in tissue sections, either in uninjured muscle or at multiple time points after injury. Additionally, there is no significant difference in the expression of DNA DSB repair proteins or globally assayed DNA damage response genes, suggesting that not only DNA DSBs, but also other types of DNA damage, do not significantly mark aged muscle stem cells. Satellite cells from DNA DSB-repair-deficient SCID mice do have an unsurprisingly higher level of innate DNA DSBs and a weakened recovery from gamma-radiation-induced DNA damage. Interestingly, they are as myogenic in vitro and in vivo as satellite cells from young wild type mice, suggesting that the inefficiency in DNA DSB repair does not directly correlate with the ability to regenerate muscle after injury. Overall, our findings suggest that a DNA DSB-repair deficiency is unlikely to be a key factor in the decline in muscle regeneration observed upon aging.

  6. THE DEVELOPMENT OF MICROSTRUCTURAL DAMAGE DURING HIGH TEMPERATURE CREEP-FATIGUE OF A NICKEL ALLOY

    SciTech Connect

    L.J. Carroll; M.C. Carroll; C. Cabet; R.N. Wright

    2013-02-01

    Alloy 617 is the leading candidate material for an Intermediate Heat Exchanger (IHX) of the Very High Temperature Reactor (VHTR). To evaluate the behavior of this material in the expected service conditions, strain-controlled cyclic tests that include hold times up to 9000 s at maximum tensile strain were conducted at 950 degrees C. The fatigue resistance decreased when a hold time was added at peak tensile strain, owing to the mechanisms resulting in a change in fracture mode from transgranular in pure fatigue to intergranular in creep–fatigue. Increases in the tensile hold duration beyond an initial value were not detrimental to the creep–fatigue resistance. An analysis of the evolving failure modes was facilitated by interrupting tests during cycling for ex situ microstructural investigation.

  7. 14 CFR 25.571 - Damage-tolerance and fatigue evaluation of structure.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., detail design, and fabrication must show that catastrophic failure due to fatigue, corrosion...; (ii) The identification of principal structural elements and detail design points, the failure of... evidence, of the principal structural elements and detail design points identified in paragraph...

  8. 14 CFR 25.571 - Damage-tolerance and fatigue evaluation of structure.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., detail design, and fabrication must show that catastrophic failure due to fatigue, corrosion...; (ii) The identification of principal structural elements and detail design points, the failure of... evidence, of the principal structural elements and detail design points identified in paragraph...

  9. Peripheral and central fatigue after muscle-damaging exercise is muscle length dependent and inversely related.

    PubMed

    Skurvydas, Albertas; Brazaitis, Marius; Kamandulis, Sigitas; Sipaviciene, Saule

    2010-08-01

    Healthy untrained men performed 10 series of 12 knee eccentric extension repetitions (EE) at 160 degrees /s. The maximal voluntary isometric contraction force of the quadriceps muscle, the maximal rate of electrically induced torque development (RTD) and relaxation (RTR), isokinetic concentric torque at 30 degrees /s, the electrostimulation-induced torque at 20 and 100Hz frequencies were established before and after EE at shorter and longer muscle lengths. Besides, voluntary activation (VA) index and central activation ratio (CAR) were tested. There was more peripheral fatigue than central after EE. We established more central fatigue as well as low frequency fatigue at a shorter muscle length compared to the longer muscle length. Relative RTD as well as relative RTR, improved after EE and did not depend on the muscle length. Finally, central fatigue is inversely significantly related with the eccentric torque reduction during eccentric exercise and with the changes in muscle torque induced by low frequency stimulation.

  10. DNA damage induces the accumulation of Tiam1 by blocking β-TrCP-dependent degradation.

    PubMed

    Zhu, Guixin; Fan, Zhongyun; Ding, Miao; Mu, Libing; Liang, Juan; Ding, Yajie; Fu, Yu; Huang, Binlu; Wu, Wei

    2014-05-30

    The Rac1/JNK cascade plays important roles in DNA damage-induced apoptosis. However, how this cascade is activated upon DNA damage remains to be fully understood. We show here that, in untreated cells, Tiam1, a Rac1-specific guanine nucleotide exchange factor, is phosphorylated by casein kinase 1 (CK1) at its C terminus, leading to Skp, Cullin, F-box-containing(β-TrCP) recognition, ubiquitination, and proteasome-mediated degradation. Upon DNA-damaging anticancer drug treatment, CK1/β-TrCP-mediated Tiam1 degradation is abolished, and the accumulated Tiam1 contributes to downstream activation of Rac1/JNK. Consistently, tumor cells overexpressing Tiam1 are hypersensitive to DNA-damaging drug treatment. In xenograft mice, Tiam1-high cells are more susceptible to doxorubicin treatment. Thus, our results uncover that inhibition of proteasome-mediated Tiam1 degradation is an upstream event leading to Rac1/JNK activation and cell apoptosis in response to DNA-damaging drug treatment.

  11. Neuronal damage and calcium accumulation following transient cerebral ischemia in the rat

    SciTech Connect

    Araki, T.; Inoue, T.; Kato, H.; Kogure, K.; Murakami, M. )

    1990-06-01

    The purpose of this study was to examine the distribution of neuronal damage following transient cerebral ischemia in the rat model of four-vessel occlusion utilizing light microscopy as well as {sup 45}Ca-autoradiography. Transient ischemia was induced for 30 min. The animals were allowed to survive for 7 d after ischemia. In the animals subjected to ischemia, the most frequently and seriously damaged areas were the paramedian region of hippocampus, the hippocampal CA1 sector, and the dorsolateral part of striatum, followed by the inferior colliculus, the substantia nigra, the frontal cortex, and the thalamus, which were moderate damaged. Furthermore, the cerebellar Purkinje neurons, the hippocampal CA4 sector, the medial geniculate body, and the hippocampal CA3 sector were slightly affected. {sup 45}Ca-autoradiographyic study also revealed calcium accumulation in the identical sites of ischemic neuronal damage, except for the frontal cortex. Regional cerebral blood flow during 10 min of ischemia was severely decreased in selectively vulnerable areas. The blood flow in the medial geniculate body, the substantia nigra, the inferior colliculus, and the cerebellum was less pronounced than that in the selectively vulnerable areas. The present study demonstrates that transient cerebral ischemia can produce significant neuronal damage not only in the selectively vulnerable regions, but also in the brainstem.

  12. Lamb wave-based damage quantification and probability of detection modeling for fatigue life assessment of riveted lap joint

    NASA Astrophysics Data System (ADS)

    He, Jingjing; Wang, Dengjiang; Zhang, Weifang

    2015-03-01

    This study presents an experimental and modeling study for damage detection and quantification in riveted lap joints. Embedded lead zirconate titanate piezoelectric (PZT) ceramic wafer-type sensors are employed to perform in-situ non-destructive testing during fatigue cyclical loading. A multi-feature integration method is developed to quantify the crack size using signal features of correlation coefficient, amplitude change, and phase change. In addition, probability of detection (POD) model is constructed to quantify the reliability of the developed sizing method. Using the developed crack size quantification method and the resulting POD curve, probabilistic fatigue life prediction can be performed to provide comprehensive information for decision-making. The effectiveness of the overall methodology is demonstrated and validated using several aircraft lap joint specimens from different manufactures and under different loading conditions.

  13. Non-contact acousto-thermal signatures in as received and fatigue damaged Ti-6Al-4V

    NASA Astrophysics Data System (ADS)

    Sathish, Shamachary; Welter, John T.; Schehl, Norm; Jata, Kumar V.

    2014-02-01

    Interaction of high amplitude acoustic waves with materials produces a small increase in the temperature that can be detected and measured using an IR camera. The changes in temperature as a function of time, due to interaction of high amplitude 20 kHz acoustics, with as received and fatigue damaged polycrystalline Ti-6Al-4V samples are compared. The maximum temperature reached by the sample has been found to increase with increasing fatigue cycles. The role of multiple physical mechanisms, responsible for conversion acoustic energy to heat, like the sample geometry (finite dimension), the microstructure (grain size), and dislocation density are examined. The theoretically evaluated temperature changes are observed to be in reasonable agreement with experimental measurements. The significance of the details of microstructure and dislocation properties needed in theoretical evaluation of temperature changes are used to explain the observed differences between experimental measurements and theoretical calculations.

  14. Accumulation of Flavonols over Hydroxycinnamic Acids Favors Oxidative Damage Protection under Abiotic Stress.

    PubMed

    Martinez, Vicente; Mestre, Teresa C; Rubio, Francisco; Girones-Vilaplana, Amadeo; Moreno, Diego A; Mittler, Ron; Rivero, Rosa M

    2016-01-01

    Efficient detoxification of reactive oxygen species (ROS) is thought to play a key role in enhancing the tolerance of plants to abiotic stresses. Although multiple pathways, enzymes, and antioxidants are present in plants, their exact roles during different stress responses remain unclear. Here, we report on the characterization of the different antioxidant mechanisms of tomato plants subjected to heat stress, salinity stress, or a combination of both stresses. All the treatments applied induced an increase of oxidative stress, with the salinity treatment being the most aggressive, resulting in plants with the lowest biomass, and the highest levels of H2O2 accumulation, lipid peroxidation, and protein oxidation. However, the results obtained from the transcript expression study and enzymatic activities related to the ascorbate-glutathione pathway did not fully explain the differences in the oxidative damage observed between salinity and the combination of salinity and heat. An exhaustive metabolomics study revealed the differential accumulation of phenolic compounds depending on the type of abiotic stress applied. An analysis at gene and enzyme levels of the phenylpropanoid metabolism concluded that under conditions where flavonols accumulated to a greater degree as compared to hydroxycinnamic acids, the oxidative damage was lower, highlighting the importance of flavonols as powerful antioxidants, and their role in abiotic stress tolerance.

  15. Accumulation of Flavonols over Hydroxycinnamic Acids Favors Oxidative Damage Protection under Abiotic Stress

    PubMed Central

    Martinez, Vicente; Mestre, Teresa C.; Rubio, Francisco; Girones-Vilaplana, Amadeo; Moreno, Diego A.; Mittler, Ron; Rivero, Rosa M.

    2016-01-01

    Efficient detoxification of reactive oxygen species (ROS) is thought to play a key role in enhancing the tolerance of plants to abiotic stresses. Although multiple pathways, enzymes, and antioxidants are present in plants, their exact roles during different stress responses remain unclear. Here, we report on the characterization of the different antioxidant mechanisms of tomato plants subjected to heat stress, salinity stress, or a combination of both stresses. All the treatments applied induced an increase of oxidative stress, with the salinity treatment being the most aggressive, resulting in plants with the lowest biomass, and the highest levels of H2O2 accumulation, lipid peroxidation, and protein oxidation. However, the results obtained from the transcript expression study and enzymatic activities related to the ascorbate-glutathione pathway did not fully explain the differences in the oxidative damage observed between salinity and the combination of salinity and heat. An exhaustive metabolomics study revealed the differential accumulation of phenolic compounds depending on the type of abiotic stress applied. An analysis at gene and enzyme levels of the phenylpropanoid metabolism concluded that under conditions where flavonols accumulated to a greater degree as compared to hydroxycinnamic acids, the oxidative damage was lower, highlighting the importance of flavonols as powerful antioxidants, and their role in abiotic stress tolerance. PMID:27379130

  16. Accumulation of Flavonols over Hydroxycinnamic Acids Favors Oxidative Damage Protection under Abiotic Stress.

    PubMed

    Martinez, Vicente; Mestre, Teresa C; Rubio, Francisco; Girones-Vilaplana, Amadeo; Moreno, Diego A; Mittler, Ron; Rivero, Rosa M

    2016-01-01

    Efficient detoxification of reactive oxygen species (ROS) is thought to play a key role in enhancing the tolerance of plants to abiotic stresses. Although multiple pathways, enzymes, and antioxidants are present in plants, their exact roles during different stress responses remain unclear. Here, we report on the characterization of the different antioxidant mechanisms of tomato plants subjected to heat stress, salinity stress, or a combination of both stresses. All the treatments applied induced an increase of oxidative stress, with the salinity treatment being the most aggressive, resulting in plants with the lowest biomass, and the highest levels of H2O2 accumulation, lipid peroxidation, and protein oxidation. However, the results obtained from the transcript expression study and enzymatic activities related to the ascorbate-glutathione pathway did not fully explain the differences in the oxidative damage observed between salinity and the combination of salinity and heat. An exhaustive metabolomics study revealed the differential accumulation of phenolic compounds depending on the type of abiotic stress applied. An analysis at gene and enzyme levels of the phenylpropanoid metabolism concluded that under conditions where flavonols accumulated to a greater degree as compared to hydroxycinnamic acids, the oxidative damage was lower, highlighting the importance of flavonols as powerful antioxidants, and their role in abiotic stress tolerance. PMID:27379130

  17. Thermomechanical Fatigue Damage/Failure Mechanisms in SCS-6/Timetal 21S [0/90](Sub S) Composite

    NASA Technical Reports Server (NTRS)

    Castelli, Michael G.

    1994-01-01

    The thermomechanical fatigue (TMF) deformation, damage, and life behaviors of SCS6/Timetal 21S (0/90)s were investigated under zero-tension conditions. In-phase (IP) and out-of-phase (OP) loadings were investigated with a temperature cycle from 150 to 650 deg C. An advanced TMF test technique was used to quantify mechanically damage progression. The technique incorporated explicit measurements of the macroscopic (1) isothermal static moduli at the temperature extremes of the TMF cycle and (2) coefficient of thermal expansion (CTE) as functions of the TMF cycles. The importance of thermal property degradation and its relevance to accurate post-test data analysis and interpretation is briefly addressed. Extensive fractography and metallography were conducted on specimens from failed and interrupted tests to characterize the extent of damage at the microstructure level. Fatigue life results indicated trends analogous to those established for similar unidirectional(0) reinforced titanium matrix composite systems. High stress IP and mid to low stress OP loading conditions were life-limiting in comparison to maximum temperature isothermal conditions. Dominant damage mechanisms changed with cycle type. Damage resulting from IP TMF conditions produced measurable decreases in static moduli but only minimal changes in the CTE. Metallography on interrupted and failed specimens revealed extensive (0) fiber cracking with sparse matrix damage. No surface initiated matrix cracks were present. Comparable OP TMF conditions initiated environment enhanced surface cracking and matrix cracking initiated at (90) fiber/matrix (F/M) interfaces. Notable static moduli and CTE degradations were measured. Fractography and metallography revealed that the transverse cracks originating from the surface and (90) F/M interfaces tended to converge and coalesce at the (0) fibers.

  18. Fatigue and Damage Tolerance Analysis of a Hybrid Composite Tapered Flexbeam

    NASA Technical Reports Server (NTRS)

    Murri, Gretchen B.; Schaff, Jeffrey R.; Dobyns, Al

    2001-01-01

    The behavior of nonlinear tapered composite flexbeams under combined axial tension and cyclic bending loading was studied using coupon test specimens and finite element (FE) analyses. The flexbeams used a hybrid material system of graphite/epoxy and glass/epoxy and had internal dropped plies, dropped in an overlapping stepwise pattern. Two material configurations, differing only in the use of glass or graphite plies in the continuous plies near the midplane, were studied. Test specimens were cut from a full-size helicopter tail-rotor flexbeam and were tested in a hydraulic load frame under combined constant axialtension load and transverse cyclic bending loads. The first determination damage observed in the specimens occurred at the area around the tip of the outermost ply-drop group in the tapered region of the flexbeam, near the thick end. Delaminations grew slowly and stably, toward the thick end of the flexbeam, at the interfaces above and below the dropped-ply region. A 2D finite element model of the flexbeam was developed. The model was analyzed using a geometrically non-linear analysis with both the ANSYS and ABAQUS FE codes. The global responses of each analysis agreed well with the test results. The ANSYS model was used to calculate strain energy release rates (G) for delaminations initiating at two different ply-ending locations. The results showed that delaminations were more inclined to grow at the locations where they were observed in the test specimens. Both ANSYS and ABAQUS were used to calculate G values associated with delamination initiating at the observed location but growing in different interfaces, either above or below the ply-ending group toward the thick end, or toward the thin end from the tip of the resin pocket. The different analysis codes generated the same trends and comparable peak values, within 5-11 % for each delamination path. Both codes showed that delamination toward the thick region was largely mode II, and toward the thin

  19. Bumetanide increases manganese accumulation in the brain of rats with liver damage.

    PubMed

    Montes, Sergio; Castro-Chávez, Armando; Florian-Soto, Circe; Heras-Romero, Yessica; Ríos, Camilo; Rivera-Mancía, Susana

    2016-03-01

    Hepatic encephalopathy is a common complication in cases of liver damage; it results from several factors, including the accumulation of toxic substances in the brain, e.g. manganese, ammonia and glutamine. We have previously reported that manganese favors ammonia and glutamine accumulation in the brain of cirrhotic rats, and we suggested that such effect could be mediated by manganese-elicited activation of the NKCC1 (Na(+)/K(+)/2Cl(-) cotransporter 1). To test this hypothesis, we used bumetanide, an NKCC1 blocker prescribed to treat ascites in cirrhotic patients; we expected that if NKCC1 was responsible for manganese-mediated ammonia buildup and the subsequent glutamine accumulation, bumetanide could counteract such effect and improve motor coordination. In addition, we considered essential to test the effect of bumetanide on manganese brain levels. We used a model of liver damage in rats, consisting in bile-duct ligation. Animals were exposed to manganese in the drinking water (1 mg/ml) for two weeks and ammonia in the food (20% w/w of ammonia acetate) during the second week after surgery. Bumetanide was administered intraperitoneally in the course of the ammonia treatment. We measured glutamine and manganese in three brain regions: frontal cortex, striatum and cerebellum. Bumetanide produced no effect on glutamine accumulation; however, because of bumetanide treatment, manganese was increased in the brain, and also the activity of gamma-glutamyl transferase in plasma; thus, we consider that the influence of bumetanide and similar diuretics on liver function and manganese homeostasis should be further studied. PMID:26851372

  20. Damage accumulation in MgO irradiated with MeV Au ions at elevated temperatures

    NASA Astrophysics Data System (ADS)

    Bachiller-Perea, Diana; Debelle, Aurélien; Thomé, Lionel; Behar, Moni

    2016-09-01

    The damage accumulation process in MgO single crystals under medium-energy heavy ion irradiation (1.2 MeV Au) at fluences up to 4 × 1014 cm-2 has been studied at three different temperatures: 573, 773, and 1073 K. Disorder depth profiles have been determined through the use of the Rutherford backscattering spectrometry in channeling configuration (RBS/C). The analysis of the RBS/C data reveals two steps in the MgO damage process, irrespective of the temperature. However, we find that for increasing irradiation temperature, the damage level decreases and the fluence at which the second step takes place increases. A shift of the damage peak at increasing fluence is observed for the three temperatures, although the position of the peak depends on the temperature. These results can be explained by an enhanced defect mobility which facilitates defect migration and may favor defect annealing. X-ray diffraction reciprocal space maps confirm the results obtained with the RBS/C technique.

  1. Damage Accumulation and Annealing in 6H-SiC Irradiated with Si+

    SciTech Connect

    Jiang, Weilin; Weber, William J.; Thevuthasan, Suntharampillai; McCready, David E.

    1998-10-01

    Damage accumulation and annealing in 6H-silicon carbide (alpha-SiC) single crystals have been studied in situ using 2.0 MeV HeRBS in a <0001>-axial channeling geometry (RBS/C). The damage was induced by 550 keV Si ion implantation (30 degrees off normal) at a temperature of -110 degrees C, and the damage recovery was investigated by subsequent isochromal annealing (20 min) over the temperature range from -110 degrees C to 900 degrees C. At ion fluences below 7.5 X 10 13 Si/cm (0.04 dpa in the damage peak), only point defects appear to be created. Furthermore, the defects on the Si sublattice can be completely recovered by thermal annealing at room temperature (RT), and recovery of defects on the C sublattice is suggested. At higher fluences of 6.6 x 10 15 Si/cm (-90 degrees C), an amorphous layer is created from the surface to a depth of 0.6 mu-m. Because of recovery processes at the buried crystalline-amorphous interface, the apparent thickness of this amorphous layer decreases slightly (<10%) with increasing temperature over the range from -90 degrees C to 600 degrees C.

  2. Tensile Creep and Fatigue of Sylramic-iBN Melt-Infiltrated SiC Matrix Composites: Retained Properties, Damage Development, and Failure Mechanisms

    NASA Technical Reports Server (NTRS)

    Morscher, Greg; Gowayed, yasser; Miller, Robert; Ojard, Greg; Ahmad, Jalees; Santhosh, Unni; John, Reji

    2008-01-01

    An understanding of the elevated temperature tensile creep, fatigue, rupture, and retained properties of ceramic matrix composites (CMC) envisioned for use in gas turbine engine applications are essential for component design and life-prediction. In order to quantify the effect of stress, time, temperature, and oxidation for a state-of-the-art composite system, a wide variety of tensile creep, dwell fatigue, and cyclic fatigue experiments were performed in air at 1204 C for the SiC/SiC CMC system consisting of Sylramic-iBN SiC fibers, BN fiber interphase coating, and slurry-cast melt-infiltrated (MI) SiC-based matrix. Tests were either taken to failure or interrupted. Interrupted tests were then mechanically tested at room temperature to determine the residual properties. The retained properties of most of the composites subjected to tensile creep or fatigue were usually within 20% of the as-produced strength and 10% of the as-produced elastic modulus. It was observed that during creep, residual stresses in the composite are altered to some extent which results in an increased compressive stress in the matrix upon cooling and a subsequent increased stress required to form matrix cracks. Microscopy of polished sections and the fracture surfaces of specimens which failed during stressed-oxidation or after the room-temperature retained property test was performed on some of the specimens in order to quantify the nature and extent of damage accumulation that occurred during the test. It was discovered that the distribution of stress-dependent matrix cracking at 1204 C was similar to the as-produced composites at room temperature; however, matrix crack growth occurred over time and typically did not appear to propagate through thickness except at final failure crack. Failure of the composites was due to either oxidation-induced unbridged crack growth, which dominated the higher stress regime (> 179 MPa) or controlled by degradation of the fibers, probably caused by

  3. Intraneuronal amyloid beta accumulation and oxidative damage to nucleic acids in Alzheimer disease.

    PubMed

    Nunomura, Akihiko; Tamaoki, Toshio; Tanaka, Koich; Motohashi, Nobutaka; Nakamura, Masao; Hayashi, Takaaki; Yamaguchi, Haruyasu; Shimohama, Shun; Lee, Hyoung-gon; Zhu, Xiongwei; Smith, Mark A; Perry, George

    2010-03-01

    In an analysis of amyloid pathology in Alzheimer disease, we used an in situ approach to identify amyloid-beta (Abeta) accumulation and oxidative damage to nucleic acids in postmortem brain tissue of the hippocampal formation from subjects with Alzheimer disease. When carboxyl-terminal-specific antibodies directed against Abeta40 and Abeta42 were used for immunocytochemical analyses, Abeta42 was especially apparent within the neuronal cytoplasm, at sites not detected by the antibody specific to Abeta-oligomer. In comparison to the Abeta42-positive neurons, neurons bearing oxidative damage to nucleic acids were more widely distributed in the hippocampus. Comparative density measurements of the immunoreactivity revealed that levels of intraneuronal Abeta42 were inversely correlated with levels of intraneuronal 8-hydroxyguanosine, an oxidized nucleoside (r=- 0.61, p<0.02). Together with recent evidence that the Abeta peptide can act as an antioxidant, these results suggest that intraneuronal accumulation of non-oligomeric Abeta may be a compensatory response in neurons to oxidative stress in Alzheimer disease.

  4. Intracellular accumulation of indium ions released from nanoparticles induces oxidative stress, proinflammatory response and DNA damage.

    PubMed

    Tabei, Yosuke; Sonoda, Akinari; Nakajima, Yoshihiro; Biju, Vasudevanpillai; Makita, Yoji; Yoshida, Yasukazu; Horie, Masanori

    2016-02-01

    Due to the widespread use of indium tin oxide (ITO), it is important to investigate its effect on human health. In this study, we evaluated the cellular effects of ITO nanoparticles (NPs), indium chloride (InCl3) and tin chloride (SnCl3) using human lung epithelial A549 cells. Transmission electron microscopy and inductively coupled plasma mass spectrometry were employed to study cellular ITO NP uptake. Interestingly, greater uptake of ITO NPs was observed, as compared with soluble salts. ITO NP species released could be divided into two types: 'indium release ITO' or 'tin release ITO'. We incubated A549 cells with indium release ITO, tin release ITO, InCl3 or SnCl2 and investigated oxidative stress, proinflammatory response, cytotoxicity and DNA damage. We found that intracellular reactive oxygen species were increased in cells incubated with indium release ITO, but not tin release ITO, InCl3 or SnCl2. Messenger RNA and protein levels of the inflammatory marker, interleukin-8, also increased following exposure to indium release ITO. Furthermore, the alkaline comet assay revealed that intracellular accumulation of indium ions induced DNA damage. Our results demonstrate that the accumulation of ionic indium, but not ionic tin, from ITO NPs in the intracellular matrix has extensive cellular effects.

  5. Intraneuronal Amyloid β Accumulation and Oxidative Damage to Nucleic Acids in Alzheimer Disease

    PubMed Central

    Nunomura, Akihiko; Tamaoki, Toshio; Tanaka, Koich; Motohashi, Nobutaka; Nakamura, Masao; Hayashi, Takaaki; Yamaguchi, Haruyasu; Shimohama, Shun; Lee, Hyoung-gon; Zhu, Xiongwei; Smith, Mark A.; Perry, George

    2010-01-01

    An in situ approach was used to identify amyloid-β (Aβ) accumulation and oxidative damage to nucleic acids in postmortem brain tissue of the hippocampal formation from subjects with Alzheimer disease. When carboxyl-terminal specific antibodies directed against Aβ40 and Aβ42 were used for immunocytochemical analyses, Aβ42 was especially apparent within the neuronal cytoplasm, at sites not detected by the antibody specific to Aβ-oligomer. In comparison to the Aβ42-positive neurons, neurons bearing oxidative damage to nucleic acids were more widely distributed in the hippocampus. Comparative density measurements of the immunoreactivity revealed that levels of intraneuronal Aβ42 were inversely correlated with levels of intraneuronal 8-hydroxyguanosine, an oxidized nucleoside (r = − 0.61, p < 0.02). Together with recent evidence that the Aβ peptide can act as an antioxidant, these results suggest that intraneuronal accumulation of non-oligomeric Aβ may be a compensatory response in neurons to oxidative stress in Alzheimer disease. PMID:20034567

  6. Characterization of Damage Progression in SCS-6/timetal 21S (0)4 Under Thermomechanical Fatigue Loadings

    NASA Technical Reports Server (NTRS)

    Castelli, Michael G.

    1994-01-01

    A detailed experimental investigation was performed at a single maximum cyclic stress (sigma max) level to physically characterize the progression of thermomechanical fatigue (lW) damage in continuously reinforced (0 deg) SCS-6/Timetal 21S, a titanium matrix composite. In-phase (IP) and out of-phase (OP) loadings were investigated at sigma max = 1000 MPa with a temperature cycle from 150 to 6500 C. Damage progression, in terms of macroscopic property degradation, was experimentally quantified through an advanced TMF test methodology which incorporates explicit measurements of the isothermal static moduli at the TMF temperature extremes and the coefficient of thermal expansion (CTE) as functions of the TMF cycles. Detailed characterization of the physical damage progression at the microstructural level was performed by interrupting multiple TMF tests at various stages of mechanical property degradation and analyzing the microstructure through extensive destructive metallography. Further, the extent of damage was also quantified through residual static strength measurements. Results indicated that damage initiation occurred very early in cyclic life (N less than 0.1Nf) for both the IP and OP TMF loadings. IP TMF damage was found to be dominated by fiber breakage with a physical damage progression in the microstructure which was difficult to quantify. OP TMF loadings produced matrix cracking exclusively associated with surface initiations. Here, damage progression was easily distinguished in terms of both the number of cracks and their relative inward progressions toward the outer fiber rows with increased cycling. The point at which the leading cracks reached the outer fiber rows (when localized fiber/matrix de-bonding and matrix crack bridging occurred) appeared to be reflected in the macroscopic property degradation curves.

  7. 14 CFR 25.571 - Damage-tolerance and fatigue evaluation of structure.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) of this section. (2) The service history of airplanes of similar structural design, taking due... strength. It must be shown by analysis, supported by test evidence, or by the service history of airplanes... TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Structure Fatigue Evaluation §...

  8. 77 FR 50576 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures; OMB Approval of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-22

    ... and Fatigue Evaluation of Composite Rotorcraft Structures,'' published in the Federal Register (76 FR... impractical. In a correction document (77 FR 4890), published February 1, 2012, the FAA revised the DATES... Evaluation of Composite Rotorcraft Structures; OMB Approval of Information Collection AGENCY:...

  9. Fatigue damage observed non-destructively in fibre composite coupon test specimens by X-ray CT

    NASA Astrophysics Data System (ADS)

    Jespersen, K. M.; Mikkelsen, L. P.

    2016-07-01

    This study presents a method for monitoring the 3D fatigue damage progression on a micro-structural level in a glass fibre/polymer coupon test specimen by means of laboratory X-ray Computed Tomography (CT). A modified mount and holder made for the standard test samples to fit into the X-ray CT scanner along with a tension clamp solution is presented. Initially, the same location of the test specimen is inspected by ex-situ X-ray CT during the fatigue loading history, which shows the damage progression on a micro-structural level. The openings of individual uni-directional (UD) fibre fractures are seen to generally increase with the number of cycles, and new regions of UD fibre fractures also appear. There are some UD fibre fractures that are difficult to detect since their opening is small. Therefore, the effect of tension on the crack visibility is examined afterwards using a tension clamp solution. With applied tension some additional cracks become visible and the openings of fibre fractures increases, which shows the importance of applied tension during the scan.

  10. Development of the Non-Destructive Evaluation System Using an Eddy Current Probe for Detection of Fatigue Damage in a Stainless Steel

    NASA Astrophysics Data System (ADS)

    Oka, M.; Yakushiji, T.; Tsuchida, Y.; Enokizono, M.

    2006-03-01

    The non-destructive evaluation system which is developed using an eddy current probe to evaluate fatigue damage in an austenitic stainless steel is reported in this paper. This probe is composed of the ferrite core and two pick-up coils connected differentially. The eddy current induced by the excitation coil is disarranged by nonuniform distribution of electromagnetic characteristics due to fatigue damage. The structural function of the eddy current probe proposed, enable to detect the eddy current disarrangement by fatigue damage. This probe detects the change of electromagnetic characteristics in the direction of X. In this paper, SUS304, a austenitic stainless steel was used as the sample. The experimental results show that the output voltage of the probe clearly depends on the number of stress cycles.

  11. Development of the Non-Destructive Evaluation System Using an Eddy Current Probe for Detection of Fatigue Damage in a Stainless Steel

    SciTech Connect

    Oka, M.; Yakushiji, T.; Tsuchida, Y.; Enokizono, M.

    2006-03-06

    The non-destructive evaluation system which is developed using an eddy current probe to evaluate fatigue damage in an austenitic stainless steel is reported in this paper. This probe is composed of the ferrite core and two pick-up coils connected differentially. The eddy current induced by the excitation coil is disarranged by nonuniform distribution of electromagnetic characteristics due to fatigue damage. The structural function of the eddy current probe proposed, enable to detect the eddy current disarrangement by fatigue damage. This probe detects the change of electromagnetic characteristics in the direction of X. In this paper, SUS304, a austenitic stainless steel was used as the sample. The experimental results show that the output voltage of the probe clearly depends on the number of stress cycles.

  12. On the fatigue life of 3D woven composite

    SciTech Connect

    Dadkhah, M.S.; Cox, B.N.

    1995-12-31

    Polymer composites with three-dimensional woven graphite fiber reinforcement (3D interlock weaves) have been tested in compression-compression fatigue under load control. As under monotonic loading, the principal mechanism of failure is kink band formation in the primary load bearing twos. Observations of kink bands and microcracking in sectioned specimens suggest that fatigue progresses by the accumulation of damage to the resin within individual tows. If it is assumed that resin damage accumulates at a rate proportional to some power of the local axial shear stress in a misaligned tow, then a simple formula follows for the cycles to kink band formation. Under load control, only a few kink bands are required for specimen failure. Then the formula is also the basis for estimates of fatigue life. Fatigue life data and measured misalignment angles, which determine the local axial shear stress, support the fatigue model.

  13. Effects of Implantation Temperature and Ion Flux on Damage Accumulation in Al-Implanted 4H-SiC

    SciTech Connect

    Zhang, Yanwen; Weber, William J.; Jiang, Weilin; Wang, Chong M.; Hallen, Anders; Possnert, Goran

    2003-02-15

    The effects of implantation temperature and ion flux on damage accumulation on both the Si and C sublattices in 4H-SiC are investigated under 1.1 MeV Al irradiation at temperatures from 150 to 450 K. The rate of damage accumulation decreases dramatically and the damage profile sharpens due to significant dynamic recovery at temperatures close to the critical temperature for amorphization. At 450 K, the relative disorder and the density of planar defects increase rapidly with the increasing ion flux, exhibiting saturation at high ion fluxes. Planar defects are generated through the agglomeration of excess Si and C interstitials during irradiation and post-irradiation annealing at 450 K. Termination of (0001) planes is attributed to the accumulation of vacancies. A volume expansion of {approx}8% is observed for the peak damage region.

  14. Detection of Fatigue Damage Prior to Crack Initiation withScanning SQUID Microscopy

    SciTech Connect

    Lee, Tae-Kyu; Morris Jr., J.W.; Lee, Seungkyun; Clarke, John

    2005-11-07

    The remanence fields of fatigued ferritic steel specimens were measured using a scanning microscope based on a high transition temperature Superconducting Quantum Interference Device (SQUID). The results show an overall increase of remanence until dislocation density saturates and an additional local remanence increase after saturation during cyclic loading. Because of the combined magnetic and spatial resolution of the SQUID microscope, these local changes of dislocation structures can be detected before a crack actually initiates, and identify the sites where crack nucleation will occur.

  15. Assessment of the service life of structural steels by using degradation models with allowance for fatigue and creep of the material

    NASA Astrophysics Data System (ADS)

    Volkov, I. A.; Egunov, V. V.; Igumnov, L. A.; Kazakov, D. A.; Korotkikh, Yu. G.; Mitenkov, F. M.

    2015-11-01

    A mathematical model is developed within the framework of equations of damaged medium mechanics to describe the processes of viscoplastic straining and damage accumulation in structural steels with allowance for fatigue and creep of the material. A model of damage summation due to interaction of low-cycle fatigue and creep of the material is proposed. Material parameters and scalar functions of equations of mechanics of damaged media are determined. Viscoplastic straining and fatigue-induced damage accumulation in 08Kh18N10T and 12Kh18N9 are studied numerically, and the data obtained are compared with available results of physical experiments.

  16. Damage Accumulation in SiC/SiC Composites with 3D Architectures

    NASA Technical Reports Server (NTRS)

    Morscher, Gregory N.; Yun, Hee-Mann; DiCarlo, James A.

    2003-01-01

    The formation and propagation of multiple matrix cracks in relatively dense ceramic matrix composites when subjected to increasing tensile stress is necessary for high strength and tough composites. However, the occurrence of matrix cracks at low stresses may limit the usefulness of some non-oxide composite systems when subjected to oxidizing environments for long times at stresses sufficient to cause matrix cracking. For SiC fiber-reinforced composites with two-dimensional woven architectures and chemically vapor infiltrated (CVI) SiC matrix and melt-infiltrated (MI) Si/SiC matrix composites, the matrix cracking behavior has been fairly well characterized for different fiber-types and woven architectures. It was found that the occurrence, degree, and growth of matrix cracks depends on the material properties of the composite constituents as well as other physical properties of the composite or architecture, e.g., matrix porosity and size of the fiber bundle. In this study, matrix cracking in SiC fiber reinforced, melt-infiltrated SiC composites with a 3D orthogonal architecture was determined for specimens tested in tension at room temperature. Acoustic emission (AE) was used to monitor the matrix cracking activity, which was later confirmed by microscopic examination of specimens that had failed. The determination of the exact location of AE demonstrated that initial cracking occurred in the matrix rich regions when a large z-direction fiber bundle was used. For specimens with large z-direction fiber tows, the earliest matrix cracking could occur at half the stress for standard 2D woven composites with similar constituents. Damage accumulation in 3D architecture composites will be compared to damage accumulation in 2D architecture composites and discussed with respect to modeling composite stress-strain behavior and use of these composites at elevated temperatures.

  17. Statistical simulation of small fatigue crack nucleation and coalescence in a lamellar TiAl alloy

    NASA Astrophysics Data System (ADS)

    Chan, Kwais; Wittkowsky, Bettina; Pfuff, Michael

    1999-05-01

    This article examines the possibility of fatigue failure as the result of fatigue crack nucleation and coalescence at stress ranges below the fatigue limit and the large crack threshold where fatigue cracks are expected not to grow. By representing the material as a two-dimensional array of beam elements, the nucleation of nonpropagating small cracks at various material locations is modeled via a statistical approach that considers fatigue crack nucleation by accumulation of damage at randomly distributed weak regions. Once nucleated, the fatigue cracks do not propagate but extend only by linking with fatigue cracks subsequently formed in the contiguous elements. Result of the computer simulation suggests that fatigue failure by crack nucleation and coalescence is feasible, but the cycles-to-coalescence is much longer than the cycles-to-initiation for the first crack. Implications of the results in fatigue life assessment based on the Kitagawa diagram are discussed for TiAl alloys.

  18. Experimental and three-dimensional finite element investigation of fatigue

    NASA Astrophysics Data System (ADS)

    Bomidi, John A. R.

    are compared. (3) Torsion fatigue of bearing steel variants: (a) Custom grips are developed and integrated with an MTS torsion test rig to undertake torsion testing of various bearing steel variants. (b) A model for torsional fatigue failure was developed and the experimental and numerical results are compared. (4) Rolling contact fatigue: (a) An improved 3D RCF model is developed which is computationally efficient and accurate compared to a previously published model. (b) The 3D fatigue model is enhanced to investigate the effects of plasticity and fatigue damage due to plastic strain accumulation on rolling contact fatigue.

  19. Fatigue Damage in Notched Composite Laminates Under Tension-Tension Cyclic Loads

    NASA Technical Reports Server (NTRS)

    Stinchcomb, W. W.; Henneke, E. G.; Reifsnider, K. L.; Kress, G. R.

    1985-01-01

    The results are given of an investigation to determine the damage states which develop in graphite epoxy laminates with center holes due to tension-tension cyclic loads, to determine the influence of stacking sequence on the initiation and interaction of damage modes and the process of damage development, and to establish the relationships between the damage states and the strength, stiffness, and life of the laminates. Two quasi-isotropic laminates were selected to give different distributions of interlaminar stresses around the hole. The laminates were tested under cyclic loads (R=0.1, 10 Hz) at maximum stresses ranging between 60 and 95 percent of the notched tensile strength.

  20. Cockayne syndrome group B protein prevents the accumulation of damaged mitochondria by promoting mitochondrial autophagy.

    PubMed

    Scheibye-Knudsen, Morten; Ramamoorthy, Mahesh; Sykora, Peter; Maynard, Scott; Lin, Ping-Chang; Minor, Robin K; Wilson, David M; Cooper, Marcus; Spencer, Richard; de Cabo, Rafael; Croteau, Deborah L; Bohr, Vilhelm A

    2012-04-01

    Cockayne syndrome (CS) is a devastating autosomal recessive disease characterized by neurodegeneration, cachexia, and accelerated aging. 80% of the cases are caused by mutations in the CS complementation group B (CSB) gene known to be involved in DNA repair and transcription. Recent evidence indicates that CSB is present in mitochondria, where it associates with mitochondrial DNA (mtDNA). We report an increase in metabolism in the CSB(m/m) mouse model and CSB-deficient cells. Mitochondrial content is increased in CSB-deficient cells, whereas autophagy is down-regulated, presumably as a result of defects in the recruitment of P62 and mitochondrial ubiquitination. CSB-deficient cells show increased free radical production and an accumulation of damaged mitochondria. Accordingly, treatment with the autophagic stimulators lithium chloride or rapamycin reverses the bioenergetic phenotype of CSB-deficient cells. Our data imply that CSB acts as an mtDNA damage sensor, inducing mitochondrial autophagy in response to stress, and that pharmacological modulators of autophagy are potential treatment options for this accelerated aging phenotype.

  1. Platelet Activation Due to Hemodynamic Shear Stresses: Damage Accumulation Model and Comparison to In Vitro Measurements

    PubMed Central

    Nobili, Matteo; Sheriff, Jawaad; Morbiducci, Umberto; Redaelli, Alberto; Bluestein, Danny

    2009-01-01

    The need to optimize the thrombogenic performance of blood recirculating cardiovascular devices, e.g., prosthetic heart valves (PHV) and ventricular assist devices (VAD), is accentuated by the fact that most of them require lifelong anticoagulation therapy that does not eliminate the risk of thromboembolic complications. The formation of thromboemboli in the flow field of these devices is potentiated by contact with foreign surfaces and regional flow phenomena that stimulate blood clotting, especially platelets. With the lack of appropriate methodology, device manufacturers do not specifically optimize for thrombogenic performance. Such optimization can be facilitated by formulating a robust numerical methodology with predictive capabilities of flow-induced platelet activation. In this study, a phenomenological model for platelet cumulative damage, identified by means of genetic algorithms (GAs), was correlated with in vitro experiments conducted in a Hemodynamic Shearing Device (HSD). Platelets were uniformly exposed to flow shear representing the lower end of the stress levels encountered in devices, and platelet activity state (PAS) was measured in response to six dynamic shear stress waveforms representing repeated passages through a device, and correlated to the predictions of the damage accumulation model. Experimental results demonstrated an increase in PAS with a decrease in “relaxation” time between pulses. The model predictions were in very good agreement with the experimental results. PMID:18204318

  2. DNA damage accumulation and TRF2 degradation in atypical Werner syndrome fibroblasts with LMNA mutations.

    PubMed

    Saha, Bidisha; Zitnik, Galynn; Johnson, Simon; Nguyen, Quyen; Risques, Rosa A; Martin, George M; Oshima, Junko

    2013-01-01

    Segmental progeroid syndromes are groups of disorders with multiple features suggestive of accelerated aging. One subset of adult-onset progeroid syndromes, referred to as atypical Werner syndrome, is caused by mutations in the LMNA gene, which encodes a class of nuclear intermediate filaments, lamin A/C. We previously described rapid telomere attrition and accelerated replicative senescence in cultured fibroblasts overexpressing mutant lamin A. In this study, we investigated the cellular phenotypes associated with accelerated telomere shortening in LMNA mutant primary fibroblasts. In early passage primary fibroblasts with R133L or L140R LMNA mutations, shelterin protein components were already reduced while cells still retained telomere lengths comparable to those of controls. There was a significant inverse correlation between the degree of abnormal nuclear morphology and the level of TRF2, a shelterin subunit, suggesting a potential causal relationship. Stabilization of the telomeres via the introduction of the catalytic subunit of human telomerase, hTERT (human telomerase reverse transcriptase), did not prevent degradation of shelterin components, indicating that reduced TRF2 in LMNA mutants is not mediated by short telomeres. Interestingly, γ-H2AX foci (reflecting double strand DNA damage) in early passage LMNA mutant primary fibroblasts and LMNA mutant hTERT fibroblasts were markedly increased in non-telomeric regions of DNA. Our results raise the possibility that mutant lamin A/C causes global genomic instability with accumulation of non-telomeric DNA damage as an early event, followed by TRF2 degradation and telomere shortening.

  3. Ametabolic embryos of Artemia franciscana accumulate DNA damage during prolonged anoxia.

    PubMed

    McLennan, Alexander G

    2009-03-01

    Encysted embryos of the brine shrimp Artemia franciscana are able to survive prolonged periods of anoxia even when fully hydrated. During this time there is no metabolism, raising the question of how embryos tolerate spontaneous, hydrolytic DNA damage such as depurination. When incubated at 28 degrees C and 40 degrees C for several weeks, hydrated anoxic embryos were found to accumulate abasic sites in their DNA with k=5.8x10(-11) s(-1) and 2.8x10(-10) s(-1), respectively. In both cases this is about 3-fold slower than expected from published observations on purified DNA. However, purified calf thymus DNA incubated under similar anoxic conditions at pH 6.3, the intracellular pH of anoxic cysts, also depurinated more slowly than predicted (about 1.7-fold), suggesting that cysts may in fact accumulate abasic sites only slightly more slowly than purified DNA. Upon reoxygenation of cysts stored under N(2) for 30 weeks at 28 degrees C, the number of abasic sites per 10(4) bp DNA fell from 21.1+/-4.0 to 9.8+/-2.0 by 12 h and to 6.2+/-2.1 by 24 h. Larvae hatched after 48 h and 72 h had only 0.59+/-0.17 and 0.48+/-0.07 abasic sites per 10(4) bp, respectively, suggesting that repair of these lesions had largely taken place before hatching commenced. Thus, unlike bacterial spores, Artemia cysts appear to have no specific protective mechanism beyond what might be afforded by chromatin structure to limit spontaneous depurination, and rely on the repair of accumulated lesions during the period between reoxygenation and hatching. PMID:19251993

  4. Quantifying the Thermal Fatigue of CPV Modules

    SciTech Connect

    Bosco, N.; Kurtz, S.

    2011-02-01

    A method is presented to quantify thermal fatigue in the CPV die-attach from meteorological data. A comparative; study between cities demonstrates a significant difference in the accumulated damage. These differences are most; sensitive to the number of larger (ΔT) thermal cycles experienced for a location. High frequency data (<1/min) may; be required to most accurately employ this method.

  5. A link between the accumulation of DNA damage and loss of multi-potency of human mesenchymal stromal cells.

    PubMed

    Alves, Hugo; Munoz-Najar, Ursula; De Wit, Jan; Renard, Auke J S; Hoeijmakers, Jan H J; Sedivy, John M; Van Blitterswijk, Clemens; De Boer, Jan

    2010-12-01

    Human mesenchymal stromal cells (hMSCs) represent an attractive cell source for clinic applications. Besides being multi-potent, recent clinical trials suggest that they secrete both trophic and immunomodulatory factors, allowing allogenic MSCs to be used in a wider variety of clinical situations. The yield of prospective isolation is however very low, making expansion a required step toward clinical applications. Unfortunately, this leads to a significant decrease in their stemness. To identify the mechanism behind loss of multi-potency, hMSCs were expanded until replicative senescence and the concomitant molecular changes were characterized at regular intervals. We observed that, with time of culture, loss of multi-potency was associated with both the accumulation of DNA damage and the respective activation of the DNA damage response pathway, suggesting a correlation between both phenomena. Indeed, exposing hMSCs to DNA damage agents led to a significant decrease in the differentiation potential. We also showed that hMSCs are susceptible to accumulate DNA damage upon in vitro expansion, and that although hMSCs maintained an effective nucleotide excision repair activity, there was a progressive accumulation of DNA damage. We propose a model in which DNA damage accumulation contributes to the loss of differentiation potential of hMSCs, which might not only compromise their potential for clinical applications but also contribute to the characteristics of tissue ageing.

  6. 14 CFR 25.571 - Damage-tolerance and fatigue evaluation of structure.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., corrosion, or accidental damage. Repeated load and static analyses supported by test evidence and (if... accidental damage. Repeated load and static analyses supported by test evidence and (if available) service... catastrophic failure of the airplane; and (iii) An analysis, supported by test evidence, of the...

  7. 14 CFR 23.573 - Damage tolerance and fatigue evaluation of structure.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... material variability and environmental conditions on the strength and durability properties of the... established that ensure the strength of each joint. (6) Structural components for which the damage tolerance... supported by test evidence. The extent of damage for residual strength evaluation at any time within...

  8. Fatigue damage estimate comparisons for northern European and US wind farm loading environments

    NASA Astrophysics Data System (ADS)

    Sutherland, H. J.; Kelley, N. D.

    Typical loading histories associated with wind turbine service environments in northern Europe and within a large wind farm in the continental US were recently compared by Kelley using the WISPER (Ten Have) loading standard and its development protocol. In this study, an equivalent load spectrum for a US wind farm was developed by applying the WISPER development protocol to representative service load histories collected from two adjacent turbines operating within a large wind farm in San Gorgonio Pass, California. The results of this study showed that turbines operating in the California wind farm experience many more loading cycles with larger peak-to-peak values for the same mean wind speed classification than their European counterparts. In this paper, the impact of the two WISPER-protocol fatigue-load spectra on service lifetime predictions are used to compare and contrast the impact of the two loading environments with one another. The service lifetime predictions are made using the LIFE2 Fatigue Analysis Code (Sutherland and Schluter), with the fatigue properties of typical fiber glass composite blade materials. Additional analyses, based on rainflow counted time histories from the San Gorgonio turbines, are also used in the comparisons. In general, these results indicate that the WISPER load spectrum from northern European sites significantly underestimates the WISPER protocol load spectrum from a US wind farm site; i.e., the WISPER load spectrum significantly underestimates the number and magnitude of the loads observed at a US wind farm site. The authors conclude that there are fundamental differences in the two service environments.

  9. Accumulation of lipids and oxidatively damaged DNA in hepatocytes exposed to particles

    SciTech Connect

    Vesterdal, Lise K.; Danielsen, Pernille H.; Folkmann, Janne K.; Jespersen, Line F.; Aguilar-Pelaez, Karin; Roursgaard, Martin; Loft, Steffen; Møller, Peter

    2014-01-15

    Exposure to particles has been suggested to generate hepatosteatosis by oxidative stress mechanisms. We investigated lipid accumulation in cultured human hepatocytes (HepG2) and rat liver after exposure to four different carbon-based particles. HepG2 cells were exposed to particles for 3 h and subsequently incubated for another 18 h to manifest lipid accumulation. In an animal model of metabolic syndrome we investigated the association between intake of carbon black (CB, 14 nm) particles and hepatic lipid accumulation, inflammation and gene expression of Srebp-1, Fasn and Scd-1 involved in lipid synthesis. There was a concentration-dependent increase in intracellular lipid content after exposure to CB in HepG2 cells, which was only observed after co-exposure to oleic/palmitic acid. Similar results were observed in HepG2 cells after exposure to diesel exhaust particles, fullerenes C{sub 60} or pristine single-walled carbon nanotubes. All four types of particles also generated oxidatively damaged DNA, assessed as formamidopyrimidine DNA glycosylase (FPG) sensitive sites, in HepG2 cells after 3 h exposure. The animal model of metabolic syndrome showed increased lipid load in the liver after one oral exposure to 6.4 mg/kg of CB in lean Zucker rats. This was not associated with increased iNOS staining in the liver, indicating that the oral CB exposure was associated with hepatic steatosis rather than steatohepatitis. The lipid accumulation did not seem to be related to increased lipogenesis because there were unaltered gene expression levels in both the HepG2 cells and rat livers. Collectively, exposure to particles is associated with oxidative stress and steatosis in hepatocytes. - Highlights: • Oral exposure to nanosized carbon black was associated with hepatosteatosis in rats. • In vitro studies included carbon black, C{sub 60}, diesel exhaust particles and SWCNTs. • Exposure to particles and free fatty acids increased lipid load in HepG2 cells. • Unaltered

  10. Simplified rotor load models and fatigue damage estimates for offshore wind turbines.

    PubMed

    Muskulus, M

    2015-02-28

    The aim of rotor load models is to characterize and generate the thrust loads acting on an offshore wind turbine. Ideally, the rotor simulation can be replaced by time series from a model with a few parameters and state variables only. Such models are used extensively in control system design and, as a potentially new application area, structural optimization of support structures. Different rotor load models are here evaluated for a jacket support structure in terms of fatigue lifetimes of relevant structural variables. All models were found to be lacking in accuracy, with differences of more than 20% in fatigue load estimates. The most accurate models were the use of an effective thrust coefficient determined from a regression analysis of dynamic thrust loads, and a novel stochastic model in state-space form. The stochastic model explicitly models the quasi-periodic components obtained from rotational sampling of turbulent fluctuations. Its state variables follow a mean-reverting Ornstein-Uhlenbeck process. Although promising, more work is needed on how to determine the parameters of the stochastic model and before accurate lifetime predictions can be obtained without comprehensive rotor simulations.

  11. Simplified rotor load models and fatigue damage estimates for offshore wind turbines.

    PubMed

    Muskulus, M

    2015-02-28

    The aim of rotor load models is to characterize and generate the thrust loads acting on an offshore wind turbine. Ideally, the rotor simulation can be replaced by time series from a model with a few parameters and state variables only. Such models are used extensively in control system design and, as a potentially new application area, structural optimization of support structures. Different rotor load models are here evaluated for a jacket support structure in terms of fatigue lifetimes of relevant structural variables. All models were found to be lacking in accuracy, with differences of more than 20% in fatigue load estimates. The most accurate models were the use of an effective thrust coefficient determined from a regression analysis of dynamic thrust loads, and a novel stochastic model in state-space form. The stochastic model explicitly models the quasi-periodic components obtained from rotational sampling of turbulent fluctuations. Its state variables follow a mean-reverting Ornstein-Uhlenbeck process. Although promising, more work is needed on how to determine the parameters of the stochastic model and before accurate lifetime predictions can be obtained without comprehensive rotor simulations. PMID:25583872

  12. Probabilistic Fatigue Damage Prognosis Using a Surrogate Model Trained Via 3D Finite Element Analysis

    NASA Technical Reports Server (NTRS)

    Leser, Patrick E.; Hochhalter, Jacob D.; Newman, John A.; Leser, William P.; Warner, James E.; Wawrzynek, Paul A.; Yuan, Fuh-Gwo

    2015-01-01

    Utilizing inverse uncertainty quantification techniques, structural health monitoring can be integrated with damage progression models to form probabilistic predictions of a structure's remaining useful life. However, damage evolution in realistic structures is physically complex. Accurately representing this behavior requires high-fidelity models which are typically computationally prohibitive. In the present work, a high-fidelity finite element model is represented by a surrogate model, reducing computation times. The new approach is used with damage diagnosis data to form a probabilistic prediction of remaining useful life for a test specimen under mixed-mode conditions.

  13. 14 CFR 27.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... effects of material and process variability along with environmental conditions in the strength and..., DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: NORMAL CATEGORY ROTORCRAFT Strength... intervals of the rotorcraft by performing damage tolerance evaluations of the strength of composite PSEs...

  14. 14 CFR 29.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... effects of material and process variability along with environmental conditions in the strength and..., DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Strength... intervals of the rotorcraft by performing damage tolerance evaluations of the strength of composite PSEs...

  15. 14 CFR 29.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... effects of material and process variability along with environmental conditions in the strength and..., DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Strength... intervals of the rotorcraft by performing damage tolerance evaluations of the strength of composite PSEs...

  16. 14 CFR 27.573 - Damage Tolerance and Fatigue Evaluation of Composite Rotorcraft Structures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... effects of material and process variability along with environmental conditions in the strength and..., DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: NORMAL CATEGORY ROTORCRAFT Strength... intervals of the rotorcraft by performing damage tolerance evaluations of the strength of composite PSEs...

  17. Accumulation of p21 proteins at DNA damage sites independent of p53 and core NHEJ factors following irradiation

    SciTech Connect

    Koike, Manabu; Yutoku, Yasutomo; Koike, Aki

    2011-08-19

    Highlights: {yields} p21 accumulated rapidly at laser-irradiated sites via its C-terminal region. {yields} p21 colocalized with the DSB marker {gamma}-H2AX and the DSB sensor Ku80. {yields} Accumulation of p21 is dependent on PCNA, but not p53 and the NHEJ core factors. {yields} Accumulation activity of p21 was conserved among human and animal cells. {yields} p21 is a useful tool as a detection marker of DNA damaged sites. -- Abstract: The cyclin-dependent kinase (CDK) inhibitor p21 plays key roles in p53-dependent DNA-damage responses, i.e., cell cycle checkpoints, senescence, or apoptosis. p21 might also play a role in DNA repair. p21 foci arise at heavy-ion-irradiated DNA-double-strand break (DSB) sites, which are mainly repaired by nonhomologous DNA-end-joining (NHEJ). However, no mechanisms of p21 accumulation at double-strand break (DSB) sites have been clarified in detail. Recent works indicate that Ku70 and Ku80 are essential for the accumulation of other NHEJ core factors, e.g., DNA-PKcs, XRCC4 and XLF, and other DNA damage response factors, e.g., BRCA1. Here, we show that p21 foci arise at laser-irradiated sites in cells from various tissues from various species. The accumulation of EGFP-p21 was detected in not only normal cells, but also transformed or cancer cells. Our results also showed that EGFP-p21 accumulated rapidly at irradiated sites, and colocalized with the DSB marker {gamma}-H2AX and with the DSB sensor protein Ku80. On the other hand, the accumulation occurred in Ku70-, Ku80-, or DNA-PKcs-deficient cell lines and in human papillomavirus 18-positive cells, whereas the p21 mutant without the PCNA-binding region (EGFP-p21(1-146)) failed to accumulate at the irradiated sites. These findings suggest that the accumulation of p21, but not functional p53 and the NHEJ core factors, is dependent on PCNA. These findings also suggest that the accumulation activity of p21 at DNA damaged sites is conserved among human and animal cells, and p21 is a useful

  18. Tendon fatigue in response to mechanical loading

    PubMed Central

    Andarawis-Puri, N.; Flatow, E. L.

    2015-01-01

    Tendinopathies are commonly attributable to accumulation of sub-rupture fatigue damage from repetitive use. Data is limited to late stage disease from patients undergoing surgery, motivating development of animal models, such as ones utilizing treadmill running or repetitive reaching, to investigate the progression of tendinopathies. We developed an in vivo model using the rat patellar tendon that allows control of the loading directly applied to the tendon. This manuscript discusses the response of tendons to fatigue loading and applications of our model. Briefly, the fatigue life of the tendon was used to define low, moderate and high levels of fatigue loading. Morphological assessment showed a progression from mild kinks to fiber disruption, for low to high level fatigue loading. Collagen expression, 1 and 3 days post loading, showed more modest changes for low and moderate than high level fatigue loading. Protein and mRNA expression of Ineterleukin-1β and MMP-13 were upregulated for moderate but not low level fatigue loading. Moderate level (7200 cycles) and 100 cycles of fatigue loading resulted in a catabolic and anabolic molecular profile respectively, at both 1 and 7 days post loading. Results suggest unique mechanisms for different levels of fatigue loading that are distinct from laceration. PMID:21625047

  19. Influence of grain orientations on the initiation of fatigue damage in an Al-Li alloy.

    PubMed

    Taylor; Zhai; Wilkinson; Martin

    1999-09-01

    The variation in microstructure and texture in a rectangular bar extruded from a billet of spray-cast 8090 Al-Li alloy has been examined. The fine grain size of the as sprayed billet and the moderate extrusion ratio ( approximately 25 : 1) were seen to cause geometric dynamic recrystallization (GDR) in regions of higher strain towards the edge of the bar. The grain morphology varied from the expected elongated grains at the centre of the bar to equiaxed grains where GDR occurred at the bar edges. A <111> + <100> double fibre texture, significantly distorted towards rolling components and varying through the bar thickness, was found using electron backscatter diffraction. Fatigue resulted in a high density of short secondary cracks, many of which had arrested at grain boundaries. The cracks preferentially nucleated in grains from the <100> fibre texture corresponding to high Schmid factors.

  20. Transgenic tobacco plants accumulating osmolytes show reduced oxidative damage under freezing stress.

    PubMed

    Parvanova, Daniela; Ivanov, Sergei; Konstantinova, Tatyana; Karanov, Emanuil; Atanassov, Atanas; Tsvetkov, Tsvetan; Alexieva, Vera; Djilianov, Dimitar

    2004-01-01

    We studied the reaction to the oxidative component of freezing in several tobacco lines, transformed with genes coding for enzymes involved in the synthesis of osmoprotectants (proline, fructan or glycine betaine) along with their wild type. The levels of some oxidative stress markers (leakage of electrolytes, hydrogen peroxide and malondialdehyde) as well as the activity of antioxidative enzymes catalase (EC 1.11.1.6.) and guaiacol peroxidase (EC 1.11.1.7.) have been followed at acclimation, 12 and 24 h freezing and at recovery. Freezing for 24 h resulted in severe damages for the wild type. A corresponding increase of electrolyte leakage, hydrogen peroxide and malondialdehyde contents, a rise of peroxidase activity and inhibition of catalase activity occurred in the non-transformants. Similar, but significantly lower trend of the same parameters has been found for the transgenic lines. Moreover, the oxidative markers returned to their normal levels when the transformants were able to recover from freezing. It could be speculated that transfer of genes, coding for accumulation of osmoprotectants, is related to reduced intensity of freezing-induced oxidative processes. Our lines and model system could serve as a good prerequisite for additional studies to gain further insights into the complex role of osmoprotectants in freezing tolerance.

  1. Frequency-domain assessment of gear-tooth bending-fatigue damage-progression using the average-log-ratio, ALR, algorithm

    NASA Astrophysics Data System (ADS)

    Mark, William D.; Hines, Jason A.

    2014-04-01

    Frequency-domain (rotational-harmonic) behavior of the average-log-ratio, ALR, gear-damage detection algorithm [MSSP 24 (2010) 2807-2823] [18] is utilized to explain behavior caused by tooth-bending-fatigue damage progression. For spur and helical gears, the strongest bending-fatigue damage contributions are typically found in the rotational-harmonic region below the tooth-meshing fundamental harmonic, where ALR increases almost monotonically with increasing damage. However, when the combined elastic/plastic deformation of a damaged tooth or teeth exceeds tooth tip/root/end relief magnitude, at tooth-contact initiation and/or termination, the lowest-order transmission-error discontinuity is changed from slope discontinuity to step discontinuity, resulting in transmission-error high-frequency (rotational-harmonic n) behavior changing from proportional to 1/n2 to proportional to 1/n, therefore indicating progression to severe damage. Decomposition of the ALR damage-detection metric into rotational-harmonic frequency bands using accelerometer recordings from a notched-tooth spiral-bevel gear test illustrates the above-described behavior, thereby showing increasing stages of damage progression prior to complete gear failure.

  2. 14 CFR 23.573 - Damage tolerance and fatigue evaluation of structure.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... damage can be expected to occur. The evaluation must incorporate repeated load and static analyses... demonstrated by tests, or by analysis supported by tests, that the structure is capable of carrying ultimate... analysis supported by tests. (3) The structure must be shown by residual strength tests, or...

  3. 14 CFR 23.573 - Damage tolerance and fatigue evaluation of structure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... damage can be expected to occur. The evaluation must incorporate repeated load and static analyses... demonstrated by tests, or by analysis supported by tests, that the structure is capable of carrying ultimate... analysis supported by tests. (3) The structure must be shown by residual strength tests, or...

  4. Quiescent hematopoietic stem cells accumulate DNA damage during aging that is repaired upon entry into cell cycle

    PubMed Central

    Inlay, Matthew A; Weissman, Irving L.; Rossi, Derrick J.

    2014-01-01

    Summary Hematopoietic stem cells (HSCs) maintain homeostasis and regenerate the blood system throughout life. It has been postulated that HSCs may be uniquely capable of preserving their genomic integrity to ensure lifelong function. To directly test this, we quantified DNA damage in HSCs and downstream progenitors from young and old mice revealing that strand breaks significantly accrue in HSCs during aging. DNA damage accumulation in HSCs was associated with broad attenuation of DNA repair and response pathways that was dependent upon HSC quiescence. Accordingly, cycling fetal HSCs and adult HSCs driven into cycle up-regulated these pathways leading to repair of strand breaks. Our results demonstrate that HSCs are not comprehensively geno-protected during aging. Rather, HSC quiescence and concomitant attenuation of DNA repair and response pathways underlies DNA damage accumulation in HSCs during aging. These results provide a potential mechanism through which pre-malignant mutations accrue in HSCs. PMID:24813857

  5. Effect of machining damage on low cycle fatigue crack initiation life in drilled holes in UdimetRTM 720

    NASA Astrophysics Data System (ADS)

    Magadanz, Christine M.

    White layer is a generic term for a light etching surface layer on metal alloys that can result under extreme deformation conditions in wear, sliding or machining. While there has been some characterization of white layer due to abusive machining, the specific effect on fatigue crack initiation life has not been well documented. This study aimed to establish a relationship between the presence of white layer due to abusive machining and fatigue crack initiation life in a wrought nickel based superalloy (Udimet ® 720). Low cycle fatigue testing was conducted on large specimens containing through holes drilled with parameters aimed at creating holes with and without white layer. Initially, Acoustic Emission monitoring technologies were used to monitor for acoustic events associated with crack initiation, however, this technology was deemed unreliable for this testing. Instead, cycles to crack initiation was determined using striation density measurements on each fracture surface to estimate the number of cycles of crack propagation, which was subtracted from the total number of cycles for the specimen. A total of sixteen specimens were tested in this manner. The results suggested that the crack initiation lives of holes machined with good machining parameters were statistically longer than crack initiation lives of holes machined with poor machining parameters. The mean initiation life of the poorly machined specimens was a factor of approximately 2 times shorter than the mean initiation life of the well machined specimens. The holes machined with good machining parameters exhibited subsurface initiations which suggested that no anomalies affected crack initiation for these specimens. It was also shown that some of the poorly machined holes exhibited subsurface initiations rather than initiations at white layer damage. These holes had better surface finish than the poorly machined specimens that did fail at white layer. The mean initiation life of the poorly

  6. Reducing fatigue damage for ships in transit through structured decision making

    USGS Publications Warehouse

    Nichols, J.M.; Fackler, P.L.; Pacifici, K.; Murphy, K.D.; Nichols, J.D.

    2014-01-01

    Research in structural monitoring has focused primarily on drawing inference about the health of a structure from the structure’s response to ambient or applied excitation. Knowledge of the current state can then be used to predict structural integrity at a future time and, in principle, allows one to take action to improve safety, minimize ownership costs, and/or increase the operating envelope. While much time and effort has been devoted toward data collection and system identification, research to-date has largely avoided the question of how to choose an optimal maintenance plan. This work describes a structured decision making (SDM) process for taking available information (loading data, model output, etc.) and producing a plan of action for maintaining the structure. SDM allows the practitioner to specify his/her objectives and then solves for the decision that is optimal in the sense that it maximizes those objectives. To demonstrate, we consider the problem of a Naval vessel transiting a fixed distance in varying sea-state conditions. The physics of this problem are such that minimizing transit time increases the probability of fatigue failure in the structural supports. It is shown how SDM produces the optimal trip plan in the sense that it minimizes both transit time and probability of failure in the manner of our choosing (i.e., through a user-defined cost function). The example illustrates the benefit of SDM over heuristic approaches to maintaining the vessel.

  7. Formation, Accumulation, and Hydrolysis of Endogenous and Exogenous Formaldehyde-Induced DNA Damage.

    PubMed

    Yu, Rui; Lai, Yongquan; Hartwell, Hadley J; Moeller, Benjamin C; Doyle-Eisele, Melanie; Kracko, Dean; Bodnar, Wanda M; Starr, Thomas B; Swenberg, James A

    2015-07-01

    Formaldehyde is not only a widely used chemical with well-known carcinogenicity but is also a normal metabolite of living cells. It thus poses unique challenges for understanding risks associated with exposure. N(2-)hydroxymethyl-dG (N(2)-HOMe-dG) is the main formaldehyde-induced DNA mono-adduct, which together with DNA-protein crosslinks (DPCs) and toxicity-induced cell proliferation, play important roles in a mutagenic mode of action for cancer. In this study, N(2)-HOMe-dG was shown to be an excellent biomarker for direct adduction of formaldehyde to DNA and the hydrolysis of DPCs. The use of inhaled [(13)CD2]-formaldehyde exposures of rats and primates coupled with ultrasensitive nano ultra performance liquid chromatography-tandem mass spectrometry permitted accurate determinations of endogenous and exogenous formaldehyde DNA damage. The results show that inhaled formaldehyde only reached rat and monkey noses, but not tissues distant to the site of initial contact. The amounts of exogenous adducts were remarkably lower than those of endogenous adducts in exposed nasal epithelium. Moreover, exogenous adducts accumulated in rat nasal epithelium over the 28-days exposure to reach steady-state concentrations, followed by elimination with a half-life (t1/2) of 7.1 days. Additionally, we examined artifact formation during DNA preparation to ensure the accuracy of nonlabeled N(2)-HOMe-dG measurements. These novel findings provide critical new data for understanding major issues identified by the National Research Council Review of the 2010 Environmental Protection Agency's Draft Integrated Risk Information System Formaldehyde Risk Assessment. They support a data-driven need for reflection on whether risks have been overestimated for inhaled formaldehyde, whereas underappreciating endogenous formaldehyde as the primary source of exposure that results in bone marrow toxicity and leukemia in susceptible humans and rodents deficient in DNA repair.

  8. Formation, Accumulation, and Hydrolysis of Endogenous and Exogenous Formaldehyde-Induced DNA Damage

    PubMed Central

    Yu, Rui; Lai, Yongquan; Hartwell, Hadley J.; Moeller, Benjamin C.; Doyle-Eisele, Melanie; Kracko, Dean; Bodnar, Wanda M.; Starr, Thomas B.; Swenberg, James A.

    2015-01-01

    Formaldehyde is not only a widely used chemical with well-known carcinogenicity but is also a normal metabolite of living cells. It thus poses unique challenges for understanding risks associated with exposure. N2-hydroxymethyl-dG (N2-HOMe-dG) is the main formaldehyde-induced DNA mono-adduct, which together with DNA-protein crosslinks (DPCs) and toxicity-induced cell proliferation, play important roles in a mutagenic mode of action for cancer. In this study, N2-HOMe-dG was shown to be an excellent biomarker for direct adduction of formaldehyde to DNA and the hydrolysis of DPCs. The use of inhaled [13CD2]-formaldehyde exposures of rats and primates coupled with ultrasensitive nano ultra performance liquid chromatography-tandem mass spectrometry permitted accurate determinations of endogenous and exogenous formaldehyde DNA damage. The results show that inhaled formaldehyde only reached rat and monkey noses, but not tissues distant to the site of initial contact. The amounts of exogenous adducts were remarkably lower than those of endogenous adducts in exposed nasal epithelium. Moreover, exogenous adducts accumulated in rat nasal epithelium over the 28-days exposure to reach steady-state concentrations, followed by elimination with a half-life (t1/2) of 7.1 days. Additionally, we examined artifact formation during DNA preparation to ensure the accuracy of nonlabeled N2-HOMe-dG measurements. These novel findings provide critical new data for understanding major issues identified by the National Research Council Review of the 2010 Environmental Protection Agency’s Draft Integrated Risk Information System Formaldehyde Risk Assessment. They support a data-driven need for reflection on whether risks have been overestimated for inhaled formaldehyde, whereas underappreciating endogenous formaldehyde as the primary source of exposure that results in bone marrow toxicity and leukemia in susceptible humans and rodents deficient in DNA repair. PMID:25904104

  9. A materials failure relation of accelerating creep as empirical description of damage accumulation

    NASA Astrophysics Data System (ADS)

    Cornelius, R. R.; Scott, P. A.

    1993-07-01

    A general materials failure relation,ddot Ω = Adot Ω ^α, describes accelerating creep of materials with rate coefficients α and A, by relating rates of deformation,dot Ω, to changes in deformation rate,ddot Ω (Voight, 1988). Time of failure can be extrapolated from inverse rate versus time data, and α and A may be derived to permit one to calculate the failure time. The method is of value for quantitative hazard assessments. Mechanisms leading to damage accumulation during accelerating creep include creep fracture by stress corrosion and power law lattice deformation. These mechanisms are examined here as phenomenologically related to the materials failure relation. Apparently, both mechanisms favour α≅, where α is the parameter of the materials failure relation controlling the sensitivity to accelerating activity. For pure shear governed by power law creep of power p, under constant load, α=2.0 and A=p. Stress corrosion is widely described by Charles' equation, relating crack velocity to stress intensity during subcritical crack growth by the stress corrosion index n. The relationship between n and α is given by α=(2 n-2)/ n. The quantitative, predictive qualities of the general materials failure relationship are illustrated with examples from Mount Toc, Italy, and Mount St. Helens, Washington. Six chevron-notched short bar experiments under constant load serve as examples for accelerating creep fracture in the laboratory. The experiments were conducted on silstones of the Ithaca formation, which show a mean stress corrosion index of n=78±24. Analysis with regard to the materials failure relation results in a mean α=2.0±0.3, which complies with the established relationship between n and α.

  10. A model for the formation of fatigue striations and its relationship with small fatigue crack growth in an aluminum alloy

    SciTech Connect

    Shyam, Amit; Lara-Curzio, Edgar

    2010-01-01

    The fatigue crack growth process involves damage accumulation and crack extension. The two sub-processes that lead to fatigue crack extension were quantified separately in a recent model for small fatigue crack growth applicable to engineering alloys. Here, we report the results of an experimental investigation to assess the assumptions of that model. The fatigue striation formation in an aluminum alloy is modeled and it is verified that the number of cycles required for striation formation is related to the cyclic crack tip opening displacement and that the striation spacing is related to the monotonic crack tip displacement. It is demonstrated that extensive cyclic crack tip plasticity in the aluminum alloy causes a reduction in the magnitude of the slope of the fatigue crack propagation curves. The implications of these results on the fatigue crack propagation lifetime calculations are identified.

  11. Isothermal Damage and Fatigue Behavior of SCS-6/Timetal 21S [0/90](Sub S) Composite at 650 Deg C

    NASA Technical Reports Server (NTRS)

    Castelli, Michael G.

    1994-01-01

    The isothermal fatigue damage and life behaviors of SCS-6/Timetal 21S (0/90)s were investigated at 650 C. Strain ratcheting and degradation of the composite's static elastic modulus were carefully monitored as functions of cycles to indicate damage progression. Extensive fractographic and metallographic analyses were conducted to determine damage/failure mechanisms. Resulting fatigue lives show considerable reductions in comparison to (0) reinforced titanium matrix composites subjected to comparable conditions. Notable stiffness degradations were found to occur after the first cycle of loading, even at relatively low maximum stress levels, where cyclic lives are greater than 25,000 cycles. This was attributed to the extremely weak fiber/matrix bond which fails under relatively low transverse loads. Stiffness degradations incurred on first cycle loadings and degradations thereafter were found to increase with increasing maximum stress. Environmental effects associated with oxidation of the (90) fiber interfaces clearly played a role in the damage mechanisms as fracture surfaces revealed environment assisted matrix cracking along the (90) fibers. Metallographic analysis indicated that all observable matrix fatigue cracks initiated at the (90) fiber/matrix interfaces. Global de-bonding in the loading direction was found along the (90) fibers. No surface initiated cracks were evident and minimal if any (0) fiber cracking was visible.

  12. Accumulation of DNA damage-induced chromatin alterations in tissue-specific stem cells: the driving force of aging?

    PubMed

    Schuler, Nadine; Rübe, Claudia E

    2013-01-01

    Accumulation of DNA damage leading to stem cell exhaustion has been proposed to be a principal mechanism of aging. Using 53BP1-foci as a marker for DNA double-strand breaks (DSBs), hair follicle stem cells (HFSCs) in mouse epidermis were analyzed for age-related DNA damage response (DDR). We observed increasing amounts of 53BP1-foci during the natural aging process independent of telomere shortening and after protracted low-dose radiation, suggesting substantial accumulation of DSBs in HFSCs. Electron microscopy combined with immunogold-labeling showed multiple small 53BP1 clusters diffusely distributed throughout the highly compacted heterochromatin of aged HFSCs, but single large 53BP1 clusters in irradiated HFSCs. These remaining 53BP1 clusters did not colocalize with core components of non-homologous end-joining, but with heterochromatic histone modifications. Based on these results we hypothesize that these lesions were not persistently unrepaired DSBs, but may reflect chromatin rearrangements caused by the repair or misrepair of DSBs. Flow cytometry showed increased activation of repair proteins and damage-induced chromatin modifications, triggering apoptosis and cellular senescence in irradiated, but not in aged HFSCs. These results suggest that accumulation of DNA damage-induced chromatin alterations, whose structural dimensions reflect the complexity of the initial genotoxic insult, may lead to different DDR events, ultimately determining the biological outcome of HFSCs. Collectively, our findings support the hypothesis that aging might be largely the remit of structural changes to chromatin potentially leading to epigenetically induced transcriptional deregulation.

  13. Nondestructive Evaluation of Metal Fatigue Using Nonlinear Acoustics

    NASA Technical Reports Server (NTRS)

    Cantrell, John H., Jr.

    2008-01-01

    Safe-life and damage-tolerant design philosophies of high performance structures have driven the development of various methods to evaluate nondestructively the accumulation of damage in such structures resulting from cyclic loading. Although many techniques have proven useful, none has been able to provide an unambiguous, quantitative assessment of damage accumulation at each stage of fatigue from the virgin state to fracture. A method based on nonlinear acoustics is shown to provide such a means to assess the state of metal fatigue. The salient features of an analytical model are presented of the microelastic-plastic nonlinearities resulting from the interaction of an acoustic wave with fatigue-generated dislocation substructures and cracks that predictably evolve during the metal fatigue process. The interaction is quantified by the material (acoustic) nonlinearity parameter extracted from acoustic harmonic generation measurements. The parameters typically increase monotonically by several hundred percent over the fatigue life of the metal, thus providing a unique measure of the state of fatigue. Application of the model to aluminum alloy 2024-T4, 410Cb stainless steel, and IN100 nickel-base superalloy specimens fatigued using different loading conditions yields good agreement between theory and experiment. Application of the model and measurement technique to the on-site inspection of steam turbine blades is discussed.

  14. Nondestructive Evaluation of Metal Fatigue Using Nonlinear Acoustics

    NASA Astrophysics Data System (ADS)

    Cantrell, John H.

    2009-03-01

    Safe-life and damage-tolerant design philosophies of high performance structures have driven the development of various methods to evaluate nondestructively the accumulation of damage in such structures resulting from cyclic loading. Although many techniques have proven useful, none has been able to provide an unambiguous, quantitative assessment of damage accumulation at each stage of fatigue from the virgin state to fracture. A method based on nonlinear acoustics is shown to provide such a means to assess the state of metal fatigue. The salient features of an analytical model are presented of the microelastic-plastic nonlinearities resulting from the interaction of an acoustic wave with fatigue-generated dislocation substructures and cracks that predictably evolve during the metal fatigue process. The interaction is quantified by the material (acoustic) nonlinearity parameter β extracted from acoustic harmonic generation measurements. The β parameters typically increase monotonically by several hundred percent over the fatigue life of the metal, thus providing a unique measure of the state of fatigue. Application of the model to aluminum alloy 2024-T4 and 410 Cb stainless steel specimens fatigued using different loading conditions yields good agreement between theory and experiment. Application of the model and measurement technique to the on-site inspection of steam turbine blades is discussed.

  15. Fatigue Life and Short Crack Behavior in Ti-6Al-4V Alloy; Interactions of Foreign Object Damage, Stress, and Temperature

    NASA Astrophysics Data System (ADS)

    Majidi, Behzad

    2008-04-01

    High-cycle fatigue (HCF) failures associated with foreign object damage (FOD) in turbine engines of military aircrafts have been of major concern for the aeronautic industry in recent years. The present work is focused on characterizing the effects of FOD on crack initiation and small crack growth of a Ti-6Al-4V alloy at ambient and also elevated temperatures. Results show that the preferred crack initiation site depends on applied stress and temperature as maximum fractions of cracks emanating from the simulated damage site, and naturally initiated cracks are observed at 25 °C under the maximum stress of 700 MPa and at 300 °C under the maximum stress of 300 MPa. The fatigue crack growth rate is influenced by increasing temperature, and the FCG rate at 300 °C is higher than that at room temperature under the same Δ K, whereas this effect for FOD-site initiated cracks is not so remarkable. This observation seems to be due to the effect of stress relaxation at 300 °C. Results also indicate that fatigue crack initiation life ( N i ) and fatigue life ( N f ) are expressed by three-parameter Weibull distribution function.

  16. Modeling Thermal Fatigue in CPV Cell Assemblies: Preprint

    SciTech Connect

    Bosco, N.; Silverman, T. J.; Kurtz, S.

    2011-07-01

    A finite element model has been created to quantify the thermal fatigue damage of the CPV die attach. Simulations are used to compare to results of empirical thermal fatigue equations originally developed for accelerated chamber cycling. While the empirical equations show promise when extrapolated to the lower temperature cycles characteristic of weather-induced temperature changes in the CPV die attach, it is demonstrated that their damage does not accumulate linearly: the damage a particular cycle contributes depends on the preceding cycles. Simulations of modeled CPV cell temperature histories provided for direct comparison of the FEM and empirical methods, and for calculation of equivalent times provided by standard accelerated test sequences.

  17. Analysis of the Static and Fatigue Strenght of a Damage Tolerant 3D-Reinforced Joining Technology on Composite Single Lap Joints

    NASA Astrophysics Data System (ADS)

    Nogueira, A. C.; Drechsler, K.; Hombergsmeier, E.

    2012-07-01

    The increasing usage of carbon fiber reinforced plastics (CFRP) in aerospace together with the constant drive for fuel efficiency and lightweight design have imposed new challenges in next generation structural assemblies and load transfer efficient joining methods. To address this issue, an innovative technology, denominated Redundant High Efficiency Assembly (RHEA) joints, is introduced as a high-performance lightweight joint that combines efficient load transfer with good damage tolerance. A review of the ongoing research involving the RHEA joint technology, its through-thickness reinforcement concept and the results of quasi-static and fatigue tensile investigations of single lap shear specimens are exposed and discussed. Improvements in ultimate static load, maximum joint deformation, damage tolerance and fatigue life are encountered when comparing the performance of the RHEA lap shear joints to co-bonded reference specimens.

  18. Acoustic Emission and Damage Accumulation for Various Woven C/SiC Composites Tested in Tension at Room Temperature

    NASA Technical Reports Server (NTRS)

    Morscher, Gregory N.; Petko, Jeanne; Kiser, James D.; Gray, Hugh R. (Technical Monitor)

    2002-01-01

    Modal acoustic emission (AE) has proven to be an excellent technique to monitor damage accumulation in ceramic matrix composites. In this study, AE was used to monitor tensile load-unload-reload hysteresis tests for a variety of C fiber reinforced, SiC matrix composites. C/SiC composites were reinforced with T300 and IM7 fibers, had C, multilayer, or pseudo-porous C interphases, and had chemical vapor infiltrated SiC or melt-infiltrated SiC matrices. All of the composites exhibited considerable AE during testing. The extent and nature of the AE activity will be analyzed and discussed in light of matrix cracking and the variety of composite constituents. It is hoped that understanding the nature of stress dependent damage accumulation in these materials can be of use in life modeling for these types of composites.

  19. Acoustic Emission and Damage Accumulation for Various Woven C/SiC Composites Tested in Tension at Room Temperature

    NASA Technical Reports Server (NTRS)

    Morscher, Gregory; Petko, Jeanne; Kiser, James D.

    2002-01-01

    Modal acoustic emission (AE) has proven to be an excellent technique to monitor damage accumulation in ceramic matrix composites. In this study, AE was used to monitor tensile load-unload-reload hysteresis tests for a variety of C fiber reinforced, Sic matrix composites. C/SiC composites were reinforced with T-300 and IM7 fibers, had C, multilayer, or pseudo-porous C interphases, and had chemical vapor infiltrated Sic or melt-infiltrated SiC matrices. All of the composites exhibited considerable AE during testing. The extent and nature of the AE activity will be analyzed and discussed in light of matrix cracking and the variety of composite constituents. It is hoped that understanding the nature of stress-dependent damage accumulation in these materials can be of use in life-modeling for these types of composites.

  20. DNA damage and metal accumulation in four tissues of feral Octopus vulgaris from two coastal areas in Portugal.

    PubMed

    Raimundo, Joana; Costa, Pedro M; Vale, Carlos; Costa, Maria Helena; Moura, Isabel

    2010-10-01

    The alkaline comet assay has been employed for the first time to estimate the basal DNA damage in the digestive gland, gills, kidney and gonads of Octopus vulgaris. Octopuses were captured in two coastal areas adjacent to the cities of Matosinhos (N) and Olhão (S), Portugal. The area of Matosinhos is influenced by discharges of the Douro River, city of Porto, industries and intensive agriculture, while Olhão is an important fisheries port. Previous works point to contrasting metal availability in the two coastal areas. Among the analysed tissues digestive gland presented the highest levels of Zn, Cu, Cd and Pb. Tissues of specimens from Matosinhos exhibited high levels of Cd and from Olhão enhanced Pb concentrations. The DNA damages in digestive gland, gills and kidney were more accentuated in specimens from Matosinhos than from Olhão, suggesting a stronger effect of contaminants. Elevated strand breakages were registered in digestive gland, recognised for its ability to store and detoxify accumulated metals. The DNA damages in kidney, gills and gonads were lower, reflecting reduced metal accumulation or efficient detoxification. The broad variability of damages in the three tissues may also mirror tissue function, specific defences to genotoxicants and cell-cycle turnover.

  1. Decreased drug accumulation and increased tolerance to DNA damage in tumor cells with a low level of cisplatin resistance.

    PubMed

    Lanzi, C; Perego, P; Supino, R; Romanelli, S; Pensa, T; Carenini, N; Viano, I; Colangelo, D; Leone, R; Apostoli, P; Cassinelli, G; Gambetta, R A; Zunino, F

    1998-04-15

    In an attempt to examine the cellular changes associated with cisplatin resistance, we selected a cisplatin-resistant (A43 1/Pt) human cervix squamous cell carcinoma cell line following continuous in vitro drug exposure. The resistant subline was characterized by a 2.5-fold degree of resistance. In particular, we investigated the expression of cellular defence systems and other cellular factors probably involved in dealing with cisplatin-induced DNA damage. Resistant cells exhibited decreased platinum accumulation and reduced levels of DNA-bound platinum and interstrand cross-link frequency after short-term drug exposure. Analysis of the effect of cisplatin on cell cycle progression revealed a cisplatin-induced G2M arrest in sensitive and resistant cells. Interestingly, a slowdown in S-phase transit was found in A431/Pt cells. A comparison of the ability of sensitive and resistant cells to repair drug-induced DNA damage suggested that resistant cells were able to tolerate higher levels of cisplatin-induced DNA damage than their parental counterparts. Analysis of the expression of proteins involved in DNA mismatch repair showed a decreased level of MSH2 in resistant cells. Since MSH2 seems to be involved in recognition of drug-induced DNA damage, this change may account for the increased tolerance to DNA damage observed in the resistant subline. In conclusion, the involvement of accumulation defects and the increased tolerance to cisplatin-induced DNA damage in these cisplatin-resistant cells support the notion that multiple changes contribute to confer a low level of cisplatin resistance. PMID:9719480

  2. An engineering approach to the prediction of fatigue behavior of unnotched/notched fiber reinforced composite laminates

    NASA Technical Reports Server (NTRS)

    Kulkarni, S. V.; Mclaughlin, P. V., Jr.

    1978-01-01

    An engineering approach is proposed for predicting unnotched/notched laminate fatigue behavior from basic lamina fatigue data. The fatigue analysis procedure was used to determine the laminate property (strength/stiffness) degradation as a function of fatigue cycles in uniaxial tension and in plane shear. These properties were then introduced into the failure model for a notched laminate to obtain damage growth, residual strength, and failure mode. The approach is thus essentially a combination of the cumulative damage accumulation (akin to the Miner-Palmgren hypothesis and its derivatives) and the damage growth rate (similar to the fracture mechanics approach) philosophies. An analysis/experiment correlation appears to confirm the basic postulates of material wearout and the predictability of laminate fatigue properties from lamina fatigue data.

  3. A Nonlinear Reduced Order Method for Prediction of Acoustic Fatigue

    NASA Technical Reports Server (NTRS)

    Przekop, Adam; Rizzi, Stephen A.

    2006-01-01

    The goal of this investigation is to assess the quality of high-cycle-fatigue life estimation via a reduced order method, for structures undergoing geometrically nonlinear random vibrations. Modal reduction is performed with several different suites of basis functions. After numerically solving the reduced order system equations of motion, the physical displacement time history is obtained by an inverse transformation and stresses are recovered. Stress ranges obtained through the rainflow counting procedure are used in a linear damage accumulation method to yield fatigue estimates. Fatigue life estimates obtained using various basis functions in the reduced order method are compared with those obtained from numerical simulation in physical degrees-of-freedom.

  4. Electrical Resistance as a NDE Technique to Monitor Processing and Damage Accumulation in SiC/SiC Composites

    NASA Technical Reports Server (NTRS)

    Smith, Craig; Morscher, Gregory N.; Xia, Zhenhai

    2008-01-01

    Ceramic matrix composites are suitable for high temperature structural applications such as turbine airfoils and hypersonic thermal protection systems. The employment of these materials in such applications is limited by the ability to process components reliable and to accurately monitor and predict damage evolution that leads to failure under stressed-oxidation conditions. Current nondestructive methods such as ultrasound, x-ray, and thermal imaging are limited in their ability to quantify small scale, transverse, in-plane, matrix cracks developed over long-time creep and fatigue conditions. Electrical resistance of SiC/SiC composites is one technique that shows special promise towards this end. Since both the matrix and the fibers are conductive, changes in matrix or fiber properties should relate to changes in electrical conductivity along the length of a specimen or part. Initial efforts to quantify the electrical resistance of different fiber and different matrix SiC/SiC composites will be presented. Also, the effect of matrix cracking on electrical resistivity for several composite systems will be presented. The implications towards electrical resistance as a technique applied to composite processing, damage detection, and life-modeling will be discussed.

  5. Detection of thermal fatigue in composites by second harmonic Lamb waves

    NASA Astrophysics Data System (ADS)

    Li, Weibin; Cho, Younho; Achenbach, Jan D.

    2012-08-01

    Composite materials which are widely used in the aerospace industry, are usually subjected to frequent variation of temperature. Thermal cyclic loading may induce material degradation. Considering the long-term service of aircraft composites and the importance of safety in the aircraft industry, even a little damage that may be accumulative via thermal fatigue is often of great concern. Therefore, there is a demand to develop non-destructive approaches to evaluate thermal fatigue damage in an early stage. Due to the sensitivity of acoustic nonlinearity to micro-damage, the nonlinear ultrasonic technique has been explored as a promising tool for early detection of micro-damage. This paper investigates an experimental scheme for characterizing thermal fatigue damage in composite laminates using second harmonic Lamb waves. The present results show a monotonic increase of acoustic nonlinearity with respect to thermal fatigue cycles. The experimental observation of the correlation between the acoustic nonlinearity and thermal fatigue cycles in carbon/epoxy laminates verifies that nonlinear Lamb waves can be used to assess thermal fatigue damage rendering improved sensitivity over conventional linear feature based non-destructive evaluation techniques. Velocity and attenuation based ultrasonic studies are carried out for comparison with the nonlinear ultrasonic approach and it is found that nonlinear acoustic parameters are more promising indicators of thermal fatigue damage than linear ones.

  6. Multi-scale simulation of radiation damage accumulation and subsequent hardening in neutron-irradiated α-Fe

    SciTech Connect

    Dunn, Aaron; Dingreville, Remi; Capolungo, Laurent

    2015-11-27

    A hierarchical methodology is introduced to predict the effects of radiation damage and irradiation conditions on the yield stress and internal stress heterogeneity developments in polycrystalline α-Fe. Simulations of defect accumulation under displacement cascade damage conditions are performed using spatially resolved stochastic cluster dynamics. The resulting void and dislocation loop concentrations and average sizes are then input into a crystal plasticity formulation that accounts for the change in critical resolved shear stress due to the presence of radiation induced defects. The simulated polycrystalline tensile tests show a good match to experimental hardening data over a wide range of irradiation doses. With this capability, stress heterogeneity development and the effect of dose rate on hardening is investigated. The model predicts increased hardening at higher dose rates for low total doses. By contrast, at doses above 10–2 dpa when cascade overlap becomes significant, the model does not predict significantly different hardening for different dose rates. In conclusion, the development of such a model enables simulation of radiation damage accumulation and associated hardening without relying on experimental data as an input under a wide range of irradiation conditions such as dose, dose rate, and temperature.

  7. Multi-scale simulation of radiation damage accumulation and subsequent hardening in neutron-irradiated α-Fe

    DOE PAGES

    Dunn, Aaron; Dingreville, Remi; Capolungo, Laurent

    2015-11-27

    A hierarchical methodology is introduced to predict the effects of radiation damage and irradiation conditions on the yield stress and internal stress heterogeneity developments in polycrystalline α-Fe. Simulations of defect accumulation under displacement cascade damage conditions are performed using spatially resolved stochastic cluster dynamics. The resulting void and dislocation loop concentrations and average sizes are then input into a crystal plasticity formulation that accounts for the change in critical resolved shear stress due to the presence of radiation induced defects. The simulated polycrystalline tensile tests show a good match to experimental hardening data over a wide range of irradiation doses.more » With this capability, stress heterogeneity development and the effect of dose rate on hardening is investigated. The model predicts increased hardening at higher dose rates for low total doses. By contrast, at doses above 10–2 dpa when cascade overlap becomes significant, the model does not predict significantly different hardening for different dose rates. In conclusion, the development of such a model enables simulation of radiation damage accumulation and associated hardening without relying on experimental data as an input under a wide range of irradiation conditions such as dose, dose rate, and temperature.« less

  8. Modelling of damage accumulation and failure of structural members subjected to strong seismic actions

    NASA Astrophysics Data System (ADS)

    Trifonov, Oleg Vladimirovich

    2009-09-01

    Following the total Lagrangian approach, an incremental formulation for three-dimensional Timoshenko beam element taking into account large displacements and rotations is developed. For the failure analysis of reinforced concrete structural members, subjected to extreme loads, a new elastoplastic damage constitutive model is proposed on the level of cross-sectional variables. The model is based on the concept of the yield surface and associated flow rule. The effects of softening and strength deterioration are accounted for by the introduction of damage variables. To assure the objectivity of the numerical simulation a non-local treatment of damage variables is implemented. Comparison to different experimental results on biaxial cyclic tests is performed. Numerical results demonstrate that the proposed model effectively reproduces softening, strength deterioration, coupling between different components of the generalized force vector and other nonlinear effects accompanying the inelastic structural response under three-dimensional seismic loading.

  9. Systemic DNA Damage Accumulation Under in Vivo Tumor Growth can be Inhibited by the Antioxidant Tempol

    PubMed Central

    Georgakilas, Alexandros G.; Redon, Christophe E.; Ferguson, Nicholas F.; Kryston, Thomas B.; Parekh, Palak; Dickey, Jennifer S.; Nakamura, Asako J.; Mitchell, James B.; Bonner, William M.; Martin, Olga A.

    2014-01-01

    Aims Recently we found that mice bearing subcutaneous non-metastatic tumors exhibited elevated levels of two types of complex DNA damage, i.e., double-strand breaks and oxidatively-induced clustered DNA lesions in various tissues throughout the body, both adjacent to and distant from the tumor site. This DNA damage was dependent on CCL2, a cytokine involved in the recruitment and activation of macrophages, suggesting that this systemic DNA damage was mediated via tumor-induced chronic inflammatory responses involving cytokines, activation of macrophages, and consequent free radical production. If free radicals are involved, then a diet containing an antioxidant may decrease the distant DNA damage. Results Here we repeated our standard protocol in cohorts of two syngeneic tumor-bearing C57BL/6NCr mice that were on a Tempol-supplemented diet. We show that double-strand break and oxidatively-induced clustered DNA lesion levels were considerably decreased, about 2-3 fold, in the majority of tissues studied from the tumor-bearing mice fed the antioxidant Tempol compared to the control tumor-bearing mice. Similar results were also observed in nude mice suggesting that the Tempol effects are independent of functioning adaptive immunity. Conclusions This is the first in vivo study demonstrating the effect of a dietary antioxidant on abscopal DNA damage in tissues distant from a localized source of genotoxic stress. These findings may be important for understanding the mechanisms of genomic instability and carcinogenesis caused by chronic stress-induced systemic DNA damage and for developing preventative strategies. PMID:25069035

  10. Continuous fatigue crack monitoring of bridges: Long-Term Electrochemical Fatigue Sensor (LTEFS)

    NASA Astrophysics Data System (ADS)

    Moshier, Monty A.; Nelson, Levi; Brinkerhoff, Ryan; Miceli, Marybeth

    2016-04-01

    Fatigue cracks in steel bridges degrade the load-carrying capacity of these structures. Fatigue damage accumulation caused by the repetitive loading of everyday truck traffic can cause small fatigue cracks initiate. Understanding the growth of these fatigue cracks is critical to the safety and reliability of our transportation infrastructure. However, modeling fatigue in bridges is difficult due to the nature of the loading and variations in connection integrity. When fatigue cracks reach critical lengths failures occur causing partial or full closures, emergency repairs, and even full structural failure. Given the aging US highway and the trend towards asset management and life extension, the need for reliable, cost effective sensors and monitoring technologies to alert bridge owners when fatigue cracks are growing is higher than ever. In this study, an innovative Long-Term Electrochemical Fatigue Sensor (LTEFS) has been developed and introduced to meet the growing NDT marketplace demand for sensors that have the ability to continuously monitor fatigue cracks. The performance of the LTEFS has been studied in the laboratory and in the field. Data was collected using machined specimens with different lengths of naturally initiated fatigue cracks, applied stress levels, applied stress ratios, and for both sinusoidal and real-life bridge spectrum type loading. The laboratory data was evaluated and used to develop an empirically based algorithm used for crack detection. Additionally, beta-tests on a real bridge structure has been completed. These studies have conclusively demonstrated that LTEFS holds great potential for long-term monitoring of fatigue cracks in steel structures

  11. The fatigue evaluation method for a structural stainless steel using the magnetic sensor composed of three pancake coils

    SciTech Connect

    Oka, M.; Tsuchida, Y.; Enokizono, M.; Yakushiji, T.

    2011-06-23

    May metallic structural materials, such as stainless steels, are currently used in our surroundings. If external force is repeatedly added for many years, it is thought that fatigue damage accumulates in stainless steels. When excessive fatigue damage accumulates in these metals, there is a possibility that they are destroyed by fatigue damage accumulation. Therefore, it is important to know the amount of the fatigue damage they have suffered in order to prevent them from being destroyed. We are developing the fatigue evaluation method for stainless steels with a magnetic sensor composed of three pancake type coils. In this research, the inspection object is ferritic stainless steels such as SUS430. The method of fatigue evaluation for ferritic stainless steels uses the three coil type sensor, and shows a good correlation between the number of stress cycles and the output signal of the sensor, even though the correlation between the output signal and an added stress is not completely accurate. This paper describes the evaluation method of fatigue damage in ferritic stainless steel using a magnetic sensor composed of three pancake-type coils.

  12. Disease Activity and Damage are not Associated with Increased Levels of Fatigue in Systemic Lupus Erythematosus Patients from LUMINA LXVII, a Multiethnic Cohort

    PubMed Central

    Burgos, Paula I.; Alarcón, Graciela S.; McGwin, Gerald; Crews, Kendra Q.; Reveille, John D.; Vilá, Luis M.

    2009-01-01

    Objective To determine the factors associated with increased levels of fatigue over the course of the disease in systemic lupus erythematosus (SLE) patients from LUMINA (Lupus in Minorities: Nature versus Nurture), a longitudinal multiethnic cohort. Methods Patients with SLE (American College of Rheumatology revised and updated criteria), age ≥16 years, disease duration ≤ 5 years at entry into the cohort (T0), of Hispanic (Texan or Puerto Rican), African America or Caucasian ethnicity, were studied. The association between socioeconomic-demographic, health behaviors, behavioral and psychological, functional and clinical characteristics and fatigue was examined using generalized estimating equations to account for the longitudinal nature of the data. Results Five-hundred and fifteen patients (~91% female) contributed 2,609 visits to these analyses; there were: 93 (18.1%) Texan Hispanics, 101 (19.6%) Puerto Rican Hispanics, 169 (32.8%) African Americans, and 152 (29.5%) Caucasians; the patients mean (SD) age and follow up time were 37.2 (12.0) and 4.7 (3.2) years, respectively. Variables associated with increased levels of fatigue in the multivariable analyses were Caucasian ethnicity, the presence of constitutional symptoms(fever, weight loss), higher levels of pain, of abnormal illness-related behaviors and of helplessness (p’s between 0.0018 and <0.0001). Conclusions The presence of pain, abnormal illness-related behaviors, helplessness and constitutional manifestations were associated with increased levels of fatigue; however, lupus specific measures, such as disease activity and damage were not. Interventions aimed at decreasing fatigue need to take into account these findings. PMID:19714612

  13. Bithermal fatigue - A link between isothermal and thermomechanical fatigue

    NASA Technical Reports Server (NTRS)

    Halford, Gary R.; Mcgaw, Michael A.; Bill, Robert C.; Fanti, Paolo D.

    1988-01-01

    A technique for bithermal fatigue testing is presented in which the tensile and compressive halves of the cycle are conducted isothermally at two significantly different temperatures. With reference to experimental results obtained for a nickel-base superalloy, B1900 + Hf, it is shown that bithermal fatigue testing is a simple alternative to thermomechanical fatigue and can provide a conservative determination of thermomechanical fatigue life for creep damage dominated failure modes. Bithermal fatigue results can be directly related to thermomechanical fatigue results through the use of an appropriate damage rule.

  14. Fatigue and fatigue crack growth processes in hard tissues: The importance of age and surface integrity

    NASA Astrophysics Data System (ADS)

    Majd, Hessam

    With the progressive increase in partially and fully dentate seniors, fracture has become an increasingly common form of restored tooth failure. Dentin undergoes progressive changes in microstructure with patient age, and studies are now suggesting that there is a reduction in fatigue strength and fatigue crack growth resistance of this tissue. This dissertation explores aging of dentin, the influence of flaws that are introduced during restorative processes on the fatigue properties of dentin, and proposes models for characterizing the damage initiation and growth process during fatigue of dentin. Results from this investigation show that the fatigue crack growth properties (Paris Law parameters (C, m) andDeltaKth) of human dentin undergo the most significant changes at a patient age of 42 years. Based on the fatigue crack growth responses, three age groups were established including young (age≤33), aged (34≤age ≤49) and old (50≤age) patients for further analysis. There were significant differences in the initiation and growth behavior between the tissues of patients from the three age groups. With regards to the influence of restorative processes, there was no influence on the quasi-static responses of dentin. However, the endurance limit of dentin treated with the dental burs (28 MPa) and abrasive air jet (35 MPa) were approximately 36% and 20% lower than that of the control (44 MPa), respectively. Both cutting processes caused a significant reduction (p≤0.0001) in fatigue strength. An accumulative damage model was developed to characterize fatigue of the control and bur treated dentin as well as provide a model for fatigue life prediction. The damage models were derived as a function of number of loading cycles (N), and ratio of applied stress to ultimate strength (r). The developed models provide estimations for the initial state of damage, the state of damage during the life, as well as the damage accumulation rate for cyclic loading of dentin

  15. Evaluating Changes in Tendon Crimp with Fatigue Loading as an ex vivo Structural Assessment of Tendon Damage

    PubMed Central

    Freedman, Benjamin R.; Zuskov, Andrey; Sarver, Joseph J.; Buckley, Mark R.; Soslowsky, Louis J.

    2015-01-01

    The complex structure of tendons relates to their mechanical properties. Previous research has associated the waviness of collagen fibers (crimp) during quasi-static tensile loading to tensile mechanics, but less is known about the role of fatigue loading on crimp properties. In this study (IACUC approved), mouse patellar tendons were fatigue loaded while an integrated plane polariscope simultaneously assessed crimp properties. We demonstrate a novel structural mechanism whereby tendon crimp amplitude and frequency are altered with fatigue loading. In particular, fatigue loading increased the crimp amplitude across the tendon width and length, and these structural alterations were shown to be both region and load dependent. The change in crimp amplitude was strongly correlated to mechanical tissue laxity (defined as the ratio of displacement and gauge length relative to the first cycle of fatigue loading assessed at constant load throughout testing), at all loads and regions evaluated. Together, this study highlights the role of fatigue loading on tendon crimp properties as a function of load applied and region evaluated, and offers an additional structural mechanism for mechanical alterations that may lead to ultimate tendon failure. PMID:25773654

  16. Evaluation of fatigue cracks using nonlinearities of acousto-ultrasonic waves acquired by an active sensor network

    NASA Astrophysics Data System (ADS)

    Zhou, Chao; Hong, Ming; Su, Zhongqing; Wang, Qiang; Cheng, Li

    2013-01-01

    There has been increasing interest in using the nonlinear features of acousto-ultrasonic (AU) waves to detect damage onset (e.g., micro-fatigue cracks) due to their high sensitivity to damage with small dimensions. However, most existing approaches are able to infer the existence of fatigue damage qualitatively, but fail to further ascertain its location and severity. A damage characterization approach, in conjunction with the use of an active piezoelectric sensor network, was established, capable of evaluating fatigue cracks in a quantitative manner (including the co-presence of multiple fatigue cracks, and their individual locations and severities). Fundamental investigations, using both experiment and enhanced finite element analysis dedicated to the simulation of nonlinear AU waves, were carried out to link the accumulation of nonlinearities extracted from high-order AU waves to the characteristic parameters of a fatigue crack. A probability-based diagnostic imaging algorithm was developed, facilitating an intuitive presentation of identification results in images. The approach was verified experimentally by evaluating multi-fatigue cracks near rivet holes of a fatigued aluminum plate, showing satisfactory precision in characterizing real, barely visible fatigue cracks. Compared with existing methods, this approach innovatively (i) uses permanently integrated active sensor networks, conducive to automatic and online health monitoring; (ii) characterizes fatigue cracks at a quantitative level; (iii) allows detection of multiple fatigue cracks; and (iv) visualizes identification results in intuitive images.

  17. Cluster dynamics models of irradiation damage accumulation in ferritic iron. I. Trap mediated interstitial cluster diffusion

    SciTech Connect

    Kohnert, Aaron A.; Wirth, Brian D.

    2015-04-21

    The microstructure that develops under low temperature irradiation in ferritic alloys is dominated by a high density of small (2–5 nm) defects. These defects have been widely observed to move via occasional discrete hops during in situ thin film irradiation experiments. Cluster dynamics models are used to describe the formation of these defects as an aggregation process of smaller clusters created as primary damage. Multiple assumptions regarding the mobility of these damage features are tested in the models, both with and without explicit consideration of such irradiation induced hops. Comparison with experimental data regarding the density of these defects demonstrates the importance of including such motions in a valid model. In particular, discrete hops inform the limited dependence of defect density on irradiation temperature observed in experiments, which the model was otherwise incapable of producing.

  18. Influence of creep damage on the low cycle thermal-mechanical fatigue behavior of two tantalum base alloys

    NASA Technical Reports Server (NTRS)

    Sheffler, K. D.; Doble, G. S.

    1972-01-01

    Low cycle fatigue tests have been performed on the tantalum base alloys T-111 and ASTAR 811C with synchronized, independently programmed temperature and strain cycling. The thermal-mechanical cycles applied fell into three basic categories: these were isothermal cycling, in-phase thermal cycling, and out-of-phase thermal cycling. In-phase cycling was defined as tensile deformation associated with high temperature and compressive deformation with low temperature, while out-of-phase thermal cycling was defined as the reverse case. The in-phase thermal cycling had a pronounced detrimental influence on the fatigue life of both alloys, with the life reduction being greater in the solid solution strengthened T-111 alloy than in the carbide strengthened ASTAR 811C alloy. The out-of-phase tests also showed pronounced effects on the fatigue life of both alloys, although not as dramatic.

  19. Experimental study of crack initiation and propagation in high- and gigacycle fatigue in titanium alloys

    SciTech Connect

    Bannikov, Mikhail E-mail: oborin@icmm.ru Oborin, Vladimir E-mail: oborin@icmm.ru Naimark, Oleg E-mail: oborin@icmm.ru

    2014-11-14

    Fatigue (high- and gigacycle) crack initiation and its propagation in titanium alloys with coarse and fine grain structure are studied by fractography analysis of fracture surface. Fractured specimens were analyzed by interferometer microscope and SEM to improve methods of monitoring of damage accumulation during fatigue test and to verify the models for fatigue crack kinetics. Fatigue strength was estimated for high cycle fatigue regime using the Luong method [1] by “in-situ” infrared scanning of the sample surface for the step-wise loading history for different grain size metals. Fine grain alloys demonstrated higher fatigue resistance for both high cycle fatigue and gigacycle fatigue regimes. Fracture surface analysis for plane and cylindrical samples was carried out using optical and electronic microscopy method. High resolution profilometry (interferometer-profiler New View 5010) data of fracture surface roughness allowed us to estimate scale invariance (the Hurst exponent) and to establish the existence of two characteristic areas of damage localization (different values of the Hurst exponent). Area 1 with diameter ∼300 μm has the pronounced roughness and is associated with damage localization hotspot. Area 2 shows less amplitude roughness, occupies the rest fracture surface and considered as the trace of the fatigue crack path corresponding to the Paris kinetics.

  20. Cluster dynamics models of irradiation damage accumulation in ferritic iron. II. Effects of reaction dimensionality

    SciTech Connect

    Kohnert, Aaron A.; Wirth, Brian D.

    2015-04-21

    The black dot damage features which develop in iron at low temperatures exhibit significant mobility during in situ irradiation experiments via a series of discrete, intermittent, long range hops. By incorporating this mobility into cluster dynamics models, the temperature dependence of such damage structures can be explained with a surprising degree of accuracy. Such motion, however, is one dimensional in nature. This aspect of the physics has not been fully considered in prior models. This article describes one dimensional reaction kinetics in the context of cluster dynamics and applies them to the black dot problem. This allows both a more detailed description of the mechanisms by which defects execute irradiation-induced hops while allowing a full examination of the importance of kinetic assumptions in accurately assessing the development of this irradiation microstructure. Results are presented to demonstrate whether one dimensional diffusion alters the dependence of the defect population on factors such as temperature and defect hop length. Finally, the size of interstitial loops that develop is shown to depend on the extent of the reaction volumes between interstitial clusters, as well as the dimensionality of these interactions.

  1. Combined effects of radiation damage and He accumulation on bubble nucleation in Gd2Ti2O7

    NASA Astrophysics Data System (ADS)

    Taylor, Caitlin A.; Patel, Maulik K.; Aguiar, Jeffery A.; Zhang, Yanwen; Crespillo, Miguel L.; Wen, Juan; Xue, Haizhou; Wang, Yongqiang; Weber, William J.

    2016-10-01

    Pyrochlores have long been considered as host phases for long-term immobilization of radioactive waste nuclides that would undergo α-decay for hundreds of thousands of years. This work utilizes ion-beam irradiations to examine the combined effects of radiation damage and He accumulation on bubble formation in Gd2Ti2O7 over relevant waste-form timescales. Helium bubbles are not observed in pre-damaged Gd2Ti2O7 implanted with 2 × 1016 He/cm2, even after post-implantation irradiations with 7 MeV Au3+ at 300, 500, and 700 K. However, He bubbles with average diameters of 1.5 nm and 2.1 nm are observed in pre-damaged (amorphous) Gd2Ti2O7 and pristine Gd2Ti2O7, respectively, after implantation of 2 × 1017 He/cm2. The critical He concentration for bubble nucleation in Gd2Ti2O7 is estimated to be 6 at.% He.

  2. Histochemical accumulation of oxidative damage products is associated with Alzheimer-like pathology in the canine.

    PubMed

    Rofina, Jaime E; Singh, Kuldeep; Skoumalova-Vesela, Alice; van Ederen, Anne Marie; van Asten, Alfonsus J A M; Wilhelm, J; Gruys, Erik

    2004-06-01

    An important lesion in Alzheimer's disease (AD) patient brains is the neurofibrillary tangle (NFT). Hyperphosphorylated tau is its major component. In a former paper we described some NFT in the canine brain. During aging, moreover, advanced glycation end products (AGE) might accumulate. Glycated tau induces lipid peroxidation in vivo and tau and AGE antigens have been mentioned to co-localize in NFT. This indicates that AGE may play an important role in Alzheimer disease (AD) by oxidation of tau. The aim of the present study was to investigate amyloid, neurofibrillary tangles, Abeta precursor protein, Abeta, tau, ubiquitin, advanced glycation end products, 4-hyroxynonenal protein and lipofuscin in a series of dogs of varying ages. The results showed a significant positive correlation between age and amyloid quantity (Congo red staining), HNE staining and lipofuscin (LF), and between amyloid quantity and HNE staining and LF. Staining for AbetaPP seemed to have a tendency to increase with age, whereas staining for tau, ubiquitin and AGE each only gave limited positive results in a proportion of the older dogs. Preliminary studies including loss of cognitive capabilities in the older dogs and chemical measurement of lipofuscin-like pigment (LFP) accumulation in brain extracts revealed an increase with old age and dementia. The Congo red, HNE and LF results suggest that deposition of amyloid with aging might be associated with formation of end products of lipid peroxidation. The finding of the limited positive signals for tau, ubiquitin and AGE in some old cases might indicate that the spontaneous brain pathology of the aged dog reveals similarities to early stages observed in AD in humans especially those with Down syndrome.

  3. Simulation and Experiment of Thermal Fatigue in the CPV Die Attach: Preprint

    SciTech Connect

    Bosco, N.; Silverman, T.; Kurtz, S.

    2012-05-01

    FEM simulation and accelerated thermal cycling have been performed for the CPV die attach. Trends in fatigue damage accumulation and equivalent test time are explored and found to be most sensitive to temperature ramp rate. Die attach crack growth is measured through cycling and found to be in excellent agreement with simulations of the inelastic strain energy accumulated. Simulations of an entire year of weather data provides for the relative ranking of fatigue damage between four cites as well as their equivalent accelerated test time.

  4. Exercise, inflammation, and fatigue in cancer survivors

    PubMed Central

    LaVoy, Emily C.P.; Fagundes, Christopher P.; Dantzer, Robert

    2016-01-01

    Cancer-related fatigue significantly disrupts normal functioning and quality of life for a substantial portion of cancer survivors, and may persist for years following cancer treatment. While the causes of persistent fatigue among cancer survivors are not yet fully understood, accumulating evidence suggests that several pathways, including chronic inflammation, autonomic imbalance, HPA-axis dysfunction, and/or mitochondrial damage, could contribute towards the disruption of normal neuronal function and result in the symptom of cancer-related fatigue. Exercise training interventions have been shown to be some of the more successful treatment options to address cancer-related fatigue. In this review, we discuss the literature regarding the causes of persistent fatigue in cancer survivors and the mechanisms by which exercise may relieve this symptom. There is still much work to be done until the prescription of exercise becomes standard practice for cancer survivors. With improvements in the quality of studies, evidenced-based exercise interventions will allow exercise scientists and oncologists to work together to treat cancer-related fatigue. PMID:26853557

  5. Ideal sinks are not always ideal. Radiation damage accumulation in nanocomposites

    SciTech Connect

    Uberuaga, Blas Pedro; Choudhury, Samrat; Caro, Alfredo

    2014-11-27

    Designing radiation tolerant materials is one of the primary challenges associated with advanced nuclear energy systems. One attractive route that has received much attention world-wide is to introduce a high density of sinks, often in the form of interfaces or secondary phases. Here, we develop a simple model of such nanocomposites and examine the ramifications of various factors on the overall radiation stability of the material. In particular, we determine how the distribution of secondary phases, the relative sink strength of those phases, and the irradiation temperature influence the radiation tolerance of the matrix. We find that the best scenario is one in which the sinks have intermediate strength, transiently trapping defects before releasing them back into the matrix.This provides new insight into the optimal properties of nanocomposites for radiation damage environments.

  6. Ideal sinks are not always ideal. Radiation damage accumulation in nanocomposites

    DOE PAGES

    Uberuaga, Blas Pedro; Choudhury, Samrat; Caro, Alfredo

    2014-11-27

    Designing radiation tolerant materials is one of the primary challenges associated with advanced nuclear energy systems. One attractive route that has received much attention world-wide is to introduce a high density of sinks, often in the form of interfaces or secondary phases. Here, we develop a simple model of such nanocomposites and examine the ramifications of various factors on the overall radiation stability of the material. In particular, we determine how the distribution of secondary phases, the relative sink strength of those phases, and the irradiation temperature influence the radiation tolerance of the matrix. We find that the best scenariomore » is one in which the sinks have intermediate strength, transiently trapping defects before releasing them back into the matrix.This provides new insight into the optimal properties of nanocomposites for radiation damage environments.« less

  7. High cycle fatigue in the transmission electron microscope

    DOE PAGES

    Bufford, Daniel C.; Stauffer, Douglas; Mook, William M.; Syed Asif, S. A.; Boyce, Brad L.; Hattar, Khalid

    2016-06-28

    One of the most common causes of structural failure in metals is fatigue induced by cyclic loading. Historically, microstructure-level analysis of fatigue cracks has primarily been performed post mortem. However, such investigations do not directly reveal the internal structural processes at work near micro- and nanoscale fatigue cracks and thus do not provide direct evidence of active microstructural mechanisms. In this paper, the tension–tension fatigue behavior of nanocrystalline Cu was monitored in real time at the nanoscale by utilizing a new capability for quantitative cyclic mechanical loading performed in situ in a transmission electron microscope (TEM). Controllable loads were appliedmore » at frequencies from one to several hundred hertz, enabling accumulations of 106 cycles within 1 h. The nanometer-scale spatial resolution of the TEM allows quantitative fatigue crack growth studies at very slow crack growth rates, measured here at ~10–12 m·cycle–1. This represents an incipient threshold regime that is well below the tensile yield stress and near the minimum conditions for fatigue crack growth. Evidence of localized deformation and grain growth within 150 nm of the crack tip was observed by both standard imaging and precession electron diffraction orientation mapping. Finally, these observations begin to reveal with unprecedented detail the local microstructural processes that govern damage accumulation, crack nucleation, and crack propagation during fatigue loading in nanocrystalline Cu.« less

  8. High Cycle Fatigue in the Transmission Electron Microscope.

    PubMed

    Bufford, Daniel C; Stauffer, Douglas; Mook, William M; Syed Asif, S A; Boyce, Brad L; Hattar, Khalid

    2016-08-10

    One of the most common causes of structural failure in metals is fatigue induced by cyclic loading. Historically, microstructure-level analysis of fatigue cracks has primarily been performed post mortem. However, such investigations do not directly reveal the internal structural processes at work near micro- and nanoscale fatigue cracks and thus do not provide direct evidence of active microstructural mechanisms. In this study, the tension-tension fatigue behavior of nanocrystalline Cu was monitored in real time at the nanoscale by utilizing a new capability for quantitative cyclic mechanical loading performed in situ in a transmission electron microscope (TEM). Controllable loads were applied at frequencies from one to several hundred hertz, enabling accumulations of 10(6) cycles within 1 h. The nanometer-scale spatial resolution of the TEM allows quantitative fatigue crack growth studies at very slow crack growth rates, measured here at ∼10(-12) m·cycle(-1). This represents an incipient threshold regime that is well below the tensile yield stress and near the minimum conditions for fatigue crack growth. Evidence of localized deformation and grain growth within 150 nm of the crack tip was observed by both standard imaging and precession electron diffraction orientation mapping. These observations begin to reveal with unprecedented detail the local microstructural processes that govern damage accumulation, crack nucleation, and crack propagation during fatigue loading in nanocrystalline Cu. PMID:27351706

  9. Cyclic deformation, fatigue and fatigue crack propagation in Ni-base alloys

    NASA Technical Reports Server (NTRS)

    Antolovich, Stephen D.; Lerch, Brad

    1989-01-01

    Ni-base superalloys' cumulative glide behavior, damage accumulation, low-cycle fatigue, and crack propagation characteristics are directly dependent on deformation behavior which is in turn a strong function of microstructural characteristics. Microstructural instabilities and environmental interactions become additional factors at elevated temperatures. An account is presently given of microstructural, chemical, and processing techniques that may be used to obtain the properties that appear most critical or desirable in specific applications.

  10. Graphene oxide induces plasma membrane damage, reactive oxygen species accumulation and fatty acid profiles change in Pichia pastoris.

    PubMed

    Zhang, Meng; Yu, Qilin; Liang, Chen; Liu, Zhe; Zhang, Biao; Li, Mingchun

    2016-10-01

    During the past couple of years, graphene nanomaterials were extremely popular among the scientists due to the promising properties in many aspects. Before the materials being well applied, we should first focus on their biosafety and toxicity. In this study, we investigated the toxicity of synthesized graphene oxide (GO) against the model industrial organism Pichia pastoris. We found that the synthesized GO showed dose-dependent toxicity to P. pastoris, through cell membrane damage and intracellular reactive oxygen species (ROS) accumulation. In response to these cell stresses, cells had normal unsaturated fatty acid (UFA) levels but increased contents of polyunsaturated fatty acid (PUFA) with up-regulation of UFA synthesis-related genes on the transcriptional level, which made it overcome the stress under GO attack. Two UFA defective strains (spt23Δ and fad12Δ) were used to demonstrate the results above. Hence, this study suggested a close connection between PUFAs and cell survival against GO. PMID:27376352

  11. Chromosome painting and the accumulation of stable cytogenetic damage with age in healthy controls

    SciTech Connect

    Tucker, J.D.; Ramsey, M.J.; Lee, D.A.

    1995-11-01

    Chromosome painting is now routinely used to identify induced stable chromosomal rearrangements, which are difficult and expensive to analyze with classical cytogenetic methods. Theoretically the inherent stability of translocations, in contrast to unstable dicentrics, enables their use as a biodosimeter for chronic and temporally-displaced exposure. To quantify the effects of adverse exposure, it is important that the baseline frequency of stable aberrations be well understood. Recently we have used chromosome painting to show that translocations accumulate with age. We have now extended this study to nearly 100 subjects ranging in age from newborns (umbilical cord bloods, n=14) to adults aged 19-79 years. All subjects were healthy, had not received chemo- or radiotherapy, and had not been occupationally or accidentally exposed to radiation or chemicals. We scored the equivalent of 1000 metaphase cells for each subject, and observed an overall average of 1.36 stable aberrations per 100 cells. Stable aberrations increased significantly with age, and were observed at frequencies of 0.19{plus_minus}0.04, 0.77{plus_minus}0.07, and 2.39{plus_minus}0.24 per 100 cells in cord blood, adults aged 19 to 49, and adults over age 50, respectively. To understand the extent that lifestyle factors influence the frequency of stable aberrations, each subject (or one parent of each newborn) completed a comprehensive questionnaire inquiring about lifestyle factors such as smoking, alcohol consumption, and dietary habits. No smoking effect is apparent in adults, however newborns whose mothers smoked during pregnancy had a 2.6-fold increase in stable aberration frequencies (p=0.033). Repeat samples from a subset of the adults suggest that individual translocation frequencies change little over a period of -3 years.

  12. Radiation-Induced Survivin Nuclear Accumulation is Linked to DNA Damage Repair

    SciTech Connect

    Capalbo, Gianni; Weiss, Christian; Reichert, Sebastian; Roedel, Claus

    2010-05-01

    Purpose: Increased expression of survivin has been identified as a negative prognostic marker in a variety of human cancers. We have previously shown that survivin is a radiation-resistance factor and that the therapeutic effect of survivin knock-down might result from an impaired DNA repair capacity. In this study, we aimed to elucidate an interrelationship between survivin's cellular localization and DNA double-strand break repair. Methods and Materials: Survivin's cellular distribution and nuclear complex formation were assayed by Western blotting of subcellular fractions, by immunofluorescence staining, and co-immunoprecipitation in SW480 colorectal cancer cells. DNA repair capacity was analyzed by kinetics of gamma-H2AX foci formation, and by DNA-dependent protein kinase (DNA-PKcs) assays in the presence of survivin-specific or nonspecific control siRNA. Results: Following irradiation, we observed a rapid nuclear accumulation of survivin and subsequent phosphorylation of the protein in the nucleus. Co-immunoprecipitation analyses from nuclear extracts revealed an interaction among survivin, Ku70, gamma-H2AX, MDC1, and DNA-PKcs that was confirmed by immunofluorescence co-localization in nuclear foci. Survivin knock down by siRNA resulted in an impaired DNA double strand break repair, as demonstrated by an increased detection of gamma-H2AX foci/nucleus at 60 min and a higher amount of residual gamma-H2AX foci at 24 hr postirradiation. Furthermore, we detected in survivin-depleted cells a hampered S2056 autophosphorylation of DNA-PKcs and a significantly decreased DNA-PKcs kinase activity. Conclusion: These data indicate that nuclear survivin is linked to DNA double-strand break repair by interaction with members of the DNA double-strand breaks repair machinery, thus regulating DNA-PKcs activity.

  13. Primary polydipsia, but not accumulated ceramide, causes lethal renal damage in saposin D-deficient mice.

    PubMed

    Hisaki, Harumi; Matsuda, Junko; Tadano-Aritomi, Keiko; Uchida, Shunya; Okinaga, Hiroko; Miyagawa, Makoto; Tamamori-Adachi, Mimi; Iizuka, Masayoshi; Okazaki, Tomoki

    2012-10-01

    Saposin D-deficient (Sap-D(-/-)) mice develop polydipsia/polyuria and die prematurely due to renal failure with robust hydronephrosis. Such symptoms emerged when they were around 3 mo of age. To investigate the pathogenesis of their water mishandling, we attempted to limit water supply and followed sequential changes of physiological and biochemical parameters. We also analyzed renal histological changes at several time points. At 3 mo old just before water restriction challenge was started, their baseline arginine vasopressin level was comparable to the wild-type (WT) level. Twenty-four-hour water deprivation and desamino d-arginine vasopressin administration improved polydipsia and polyuria to certain degrees. However, creatinine concentrations in Sap-D(-/-) mice were significantly higher than those in WT mice, suggesting that some renal impairment already emerged in the affected mice at this age. Renal histological analyses revealed that renal tubules and collecting ducts were expanded after 3 mo old. After 6 mo old, vacuolar formation was observed, many inflammatory cells migrated around the ducts, and epithelial monolayer cells of tubular origin were replaced by plentiful cysts of various sizes. At 10∼12 mo old, severe cystic deformity appeared. On the other hand, 8-mo-long water restriction started at 4 mo old dramatically improved tubular damage and restored once-dampened amount of tubular aquaporin2 protein to the WT level. Furthermore, 10-mo-long water restriction ameliorated their renal function. Remarkably, by continuing water restriction thereafter, overall survival period became comparable with that of the WT. Together, polyuria, devastating renal tubular lesions, and renal failure were ameliorated by the mere 10-mo-long water restriction, which would trigger lethal dehydration if the disease were to be caused by any processes other than primary polydipsia. Our study demonstrates that long-term water restriction surely improved renal

  14. A phenomenological model for predicting fatigue life in bovine trabecular bone.

    PubMed

    Ganguly, P; Moore, T L A; Gibson, L J

    2004-06-01

    Cyclic loading of bone during daily activities can lead to fatigue degradation and increased risk of fracture in both the young and elderly population. Damage processes under cyclic loading in trabecular bone result in the reduction of the elastic modulus and accumulation of residual strain. These effects increase with increasing stress levels, leading to a progressive reduction in fatigue life. The present work analyzes the effect of stress and strain variation on the above damage processes in bovine trabecular bone, and develops a phenomenological model relating fatigue life to the imposed stress level. The elastic modulus reduction of the bone specimens was observed to depend on the maximum compressive strain, while the rate of residual strain accumulation was a function of the stress level. A model was developed for the upper and lower bounds of bone elastic modulus reduction with increasing number of cycles, at each stress range. The experimental observations were described well by the model. The model predicted the bounds of the fatigue life with change in fatigue stress. The decrease in the fatigue life with increasing stress was related to corresponding increases in the residual strain accumulation rates at the elevated stress levels. The model shows the validity of fatigue predictions from relatively few cyclic experiments, by combining trends observed in the monotonic and the cyclic tests. The model also presents a relatively simple procedure for predicting the endurance limit for bovine trabecular bone specimens.

  15. Mercury Accumulation, Structural Damages, and Antioxidant and Immune Status Changes in the Gilthead Seabream (Sparus aurata L.) Exposed to Methylmercury.

    PubMed

    Guardiola, F A; Chaves-Pozo, E; Espinosa, C; Romero, D; Meseguer, J; Cuesta, A; Esteban, M A

    2016-05-01

    In aquatic systems, mercury (Hg) is an environmental contaminant that causes acute and chronic damage to multiple organs. In fish, practically all of the organic Hg found is in the form of methylmercury (MeHg), which has been associated with animal and human health problems. This study evaluates the impact of waterborne-exposure to sublethal concentrations of MeHg (10 μg L(-1)) in gilthead seabream (Sparus aurata). Hg was seen to accumulate in liver and muscle, and histopathological damage to skin and liver was detected. Fish exposed to MeHg showed a decreased biological antioxidant potential and increased levels of the reactive oxygen molecules compared with the values found in control fish (nonexposed). Increased liver antioxidant enzyme activities (superoxide dismutase and catalase) were detected in 2 day-exposed fish with respect to the values of control fish. However, fish exposed to MeHg for 10 days showed liver antioxidant enzyme levels similar to those of the control fish but had increased hepato-somatic index and histopathological alterations in liver and skin. Serum complement levels were higher in fish exposed to MeHg for 30 days than in control fish. Moreover, head-kidney leukocyte activities increased, although only phagocytosis and peroxidase activities showed a significant increase after 10 and 30 days, respectively. The data show that 30 days of exposure to waterborne MeHg provokes more significant changes in fish than a short-term exposure of 2 or 10 days. PMID:26906265

  16. Dual mechanism of brain damage induced in vivo by the major metabolites accumulating in hyperornithinemia-hyperammonemia-homocitrullinuria syndrome.

    PubMed

    Viegas, Carolina Maso; Busanello, Estela Natacha Brandt; Tonin, Anelise Miotti; de Moura, Alana Pimentel; Grings, Mateus; Ritter, Luciana; Schuck, Patrícia Fernanda; Ferreira, Gustavo da Costa; Sitta, Angela; Vargas, Carmen Regla; Wajner, Moacir

    2011-01-19

    Hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome is an autosomal recessive disorder caused by a defect in the mitochondrial ornithine transporter, leading to accumulation of ornithine (Orn), homocitrulline (Hcit) and ammonia. Progressive neurological regression whose pathogenesis is not well established is common in this disease. The present work investigated the in vivo effects of intracerebroventricular administration of Orn and Hcit on important parameters of oxidative stress and energy metabolism in cerebral cortex from young rats. Orn and Hcit significantly increased thiobarbituric acid-reactive substances values and carbonyl formation, indicators of lipid and protein oxidative damage, respectively. Furthermore, N-acetylcysteine and the combination of the free radical scavengers ascorbic acid plus α-tocopherol attenuated the lipid oxidation and totally prevented the protein oxidative damage provoked by Orn and Hcit, suggesting that reactive species were involved in these effects. Hcit, but not Orn administration, also decreased glutathione concentrations, as well as the activity of catalase and glutathione peroxidase, indicating that Hcit provokes a reduction of brain antioxidant defenses. As regards to the parameters of energy metabolism, we verified that Orn and Hcit significantly inhibited the citric acid cycle function (inhibition of CO(2) synthesis from [1-(14)C] acetate), the aerobic glycolytic pathway (reduced CO(2) production from [U-(14)C] glucose) and complex I-III activity of the respiratory chain. Hcit also inhibited the activity of aconitase, an enzyme very susceptible to free radical attack. Taken together, our data indicate that mitochondrial homeostasis is disturbed by Orn and especially by Hcit. It is presumed that the impairment of brain bioenergetics and the oxidative damage induced by these metabolites may possibly contribute to the brain deterioration and neurological symptoms affecting patients with HHH syndrome. PMID

  17. Fatigue associated with prolonged graded running.

    PubMed

    Giandolini, Marlene; Vernillo, Gianluca; Samozino, Pierre; Horvais, Nicolas; Edwards, W Brent; Morin, Jean-Benoît; Millet, Guillaume Y

    2016-10-01

    Scientific experiments on running mainly consider level running. However, the magnitude and etiology of fatigue depend on the exercise under consideration, particularly the predominant type of contraction, which differs between level, uphill, and downhill running. The purpose of this review is to comprehensively summarize the neurophysiological and biomechanical changes due to fatigue in graded running. When comparing prolonged hilly running (i.e., a combination of uphill and downhill running) to level running, it is found that (1) the general shape of the neuromuscular fatigue-exercise duration curve as well as the etiology of fatigue in knee extensor and plantar flexor muscles are similar and (2) the biomechanical consequences are also relatively comparable, suggesting that duration rather than elevation changes affects neuromuscular function and running patterns. However, 'pure' uphill or downhill running has several fatigue-related intrinsic features compared with the level running. Downhill running induces severe lower limb tissue damage, indirectly evidenced by massive increases in plasma creatine kinase/myoglobin concentration or inflammatory markers. In addition, low-frequency fatigue (i.e., excitation-contraction coupling failure) is systematically observed after downhill running, although it has also been found in high-intensity uphill running for different reasons. Indeed, low-frequency fatigue in downhill running is attributed to mechanical stress at the interface sarcoplasmic reticulum/T-tubule, while the inorganic phosphate accumulation probably plays a central role in intense uphill running. Other fatigue-related specificities of graded running such as strategies to minimize the deleterious effects of downhill running on muscle function, the difference of energy cost versus heat storage or muscle activity changes in downhill, level, and uphill running are also discussed. PMID:27456477

  18. Tension fatigue analysis and life prediction for composite laminates

    NASA Technical Reports Server (NTRS)

    Obrien, T. K.; Rigamonti, M.; Zanotti, C.

    1988-01-01

    A tension fatigue life prediction methodology for composite laminates is presented. Tension fatigue tests were conducted on quasi-isotropic and orthotropic glass epoxy, graphite epoxy, and glass/graphite epoxy hybrid laminates. Edge delamination onset data were used to generate plots of strain energy release rate as a function of cycles to delamination onset. These plots were then used along with strain energy release rate analyses of delaminations initiating at matrix cracks to predict local delamination onset. Stiffness loss was measured experimentally to account for the accumulation of matrix cracks and for delamination growth. Fatigue failure was predicted by comparing the increase in global strain resulting from stiffness loss to the decrease in laminate failure strain resulting from delaminations forming at matrix cracks through the laminate thickness. Good agreement between measured and predicted lives indicated that the through-thickness damage accumulation model can accurately describe fatigue failure for laminates where the delamination onset behavior in fatigue is well characterized, and stiffness loss can be monitored in real time to account for damage growth.

  19. Fatigue loading of tendon

    PubMed Central

    Shepherd, Jennifer H; Screen, Hazel R C

    2013-01-01

    Tendon injuries, often called tendinopathies, are debilitating and painful conditions, generally considered to develop as a result of tendon overuse. The aetiology of tendinopathy remains poorly understood, and whilst tendon biopsies have provided some information concerning tendon appearance in late-stage disease, there is still little information concerning the mechanical and cellular events associated with disease initiation and progression. Investigating this in situ is challenging, and numerous models have been developed to investigate how overuse may generate tendon fatigue damage and how this may relate to tendinopathy conditions. This article aims to review these models and our current understanding of tendon fatigue damage. We review the strengths and limitations of different methodologies for characterizing tendon fatigue, considering in vitro methods that adopt both viable and non-viable samples, as well as the range of different in vivo approaches. By comparing data across model systems, we review the current understanding of fatigue damage development. Additionally, we compare these findings with data from tendinopathic tissue biopsies to provide some insights into how these models may relate to the aetiology of tendinopathy. Fatigue-induced damage consistently highlights the same microstructural, biological and mechanical changes to the tendon across all model systems and also correlates well with the findings from tendinopathic biopsy tissue. The multiple testing routes support matrix damage as an important contributor to tendinopathic conditions, but cellular responses to fatigue appear complex and often contradictory. PMID:23837793

  20. Subcritical fatigue in fuse networks

    NASA Astrophysics Data System (ADS)

    Oliveira, C. L. N.; Vieira, A. P.; Herrmann, H. J.; Andrade, J. S., Jr.

    2012-11-01

    We obtain the Paris law of fatigue crack propagation in a fuse network model where the accumulated damage in each resistor increases with time as a power law of the local current amplitude. When a resistor reaches its fatigue threshold, it burns irreversibly. Over time, this drives cracks to grow until the system is fractured into two parts. We study the relation between the macroscopic exponent of the crack-growth rate —entering the phenomenological Paris law— and the microscopic damage accumulation exponent, γ, under the influence of disorder. The way the jumps of the growing crack, Δa, and the waiting time between successive breaks, Δt, depend on the type of material, via γ, are also investigated. We find that the averages of these quantities, \\left \\langle \\Delta a\\right \\rangle and \\left \\langle \\Delta t\\right \\rangle /\\left \\langle t_r\\right \\rangle , scale as power laws of the crack length a, \\left \\langle \\Delta a\\right \\rangle \\propto a^{\\alpha } and \\left \\langle \\Delta t\\right \\rangle /\\left \\langle t_r\\right \\rangle \\propto a^{-\\beta } , where \\left \\langle t_r\\right \\rangle is the average rupture time. Strikingly, our results show, for small values of γ, a decrease in the exponent of the Paris law in comparison with the homogeneous case, leading to an increase in the lifetime of breaking materials. For the particular case of γ = 0, when fatigue is exclusively ruled by disorder, an analytical treatment confirms the results obtained by simulation.

  1. Long Term Aggresome Accumulation Leads to DNA Damage, p53-dependent Cell Cycle Arrest, and Steric Interference in Mitosis.

    PubMed

    Lu, Meng; Boschetti, Chiara; Tunnacliffe, Alan

    2015-11-13

    Juxtanuclear aggresomes form in cells when levels of aggregation-prone proteins exceed the capacity of the proteasome to degrade them. It is widely believed that aggresomes have a protective function, sequestering potentially damaging aggregates until these can be removed by autophagy. However, most in-cell studies have been carried out over a few days at most, and there is little information on the long term effects of aggresomes. To examine these long term effects, we created inducible, single-copy cell lines that expressed aggregation-prone polyglutamine proteins over several months. We present evidence that, as perinuclear aggresomes accumulate, they are associated with abnormal nuclear morphology and DNA double-strand breaks, resulting in cell cycle arrest via the phosphorylated p53 (Ser-15)-dependent pathway. Further analysis reveals that aggresomes can have a detrimental effect on mitosis by steric interference with chromosome alignment, centrosome positioning, and spindle formation. The incidence of apoptosis also increased in aggresome-containing cells. These severe defects developed gradually after juxtanuclear aggresome formation and were not associated with small cytoplasmic aggregates alone. Thus, our findings demonstrate that, in dividing cells, aggresomes are detrimental over the long term, rather than protective. This suggests a novel mechanism for polyglutamine-associated developmental and cell biological abnormalities, particularly those with early onset and non-neuronal pathologies.

  2. Self-Repairing Fatigue Damage in Metallic Structures for Aerospace Vehicles Using Shape Memory Alloy Self-healing (SMASH) Technology

    NASA Technical Reports Server (NTRS)

    Wright, M. Clara; Manuel, Michele; Wallace, Terryl; Newman, Andy; Brinson, Kate

    2015-01-01

    This DAA is for the Phase II webinar presentation of the ARMD-funded SMASH technology. A self-repairing aluminum-based composite system has been developed using liquid-assisted healing theory in conjunction with the shape memory effect of wire reinforcements. The metal matrix composite was thermodynamically designed to have a matrix with a relatively even dispersion of low-melting phase, allowing for repair of cracks at a pre-determined temperature. Shape memory alloy wire reinforcements were used within the composite to provide crack closure. Investigators focused the research on fatigue cracks propagating through the matrix in order to optimize and computer model the SMASH technology for aeronautical applications.

  3. Fatigue Crack Growth Analysis Under Spectrum Loading in Various Environmental Conditions

    NASA Astrophysics Data System (ADS)

    Mikheevskiy, S.; Glinka, G.; Lee, E.

    2013-03-01

    The fatigue process consists, from the engineering point of view, of three stages: crack initiation, fatigue crack growth, and the final failure. It is also known that the fatigue process near notches and cracks is governed by local strains and stresses in the regions of maximum stress and strain concentrations. Therefore, the fatigue crack growth can be considered as a process of successive crack increments, and the fatigue crack initiation and subsequent growth can be modeled as one repetitive process. The assumptions mentioned above were used to derive a fatigue crack growth model based, called later as the UniGrow model, on the analysis of cyclic elastic-plastic stresses-strains near the crack tip. The fatigue crack growth rate was determined by simulating the cyclic stress-strain response in the material volume adjacent to the crack tip and calculating the accumulated fatigue damage in a manner similar to fatigue analysis of stationary notches. The fatigue crack growth driving force was derived on the basis of the stress and strain history at the crack tip and the Smith-Watson-Topper (SWT) fatigue damage parameter, D = σmaxΔɛ/2. It was subsequently found that the fatigue crack growth was controlled by a two-parameter driving force in the form of a weighted product of the stress intensity range and the maximum stress intensity factor, Δ K p K {max/1- p }. The effect of the internal (residual) stress induced by the reversed cyclic plasticity has been accounted for and therefore the two-parameter driving force made it possible to predict the effect of the mean stress including the influence of the applied compressive stress, tensile overloads, and variable amplitude spectrum loading. It allows estimating the fatigue life under variable amplitude loading without using crack closure concepts. Several experimental fatigue crack growth datasets obtained for the Al 7075 aluminum alloy were used for the verification of the proposed unified fatigue crack growth

  4. Small fatigue crack growth in metallic materials: A model and its application to engineering alloys

    SciTech Connect

    Shyam, Amit

    2007-01-01

    Characterization of the growth behavior of small fatigue cracks is important for materials used in structurally demanding applications such as aircraft turbine discs and some automotive engine components. Here, we present a general, dislocation-based fracture mechanics approach to predict the growth rate of small fatigue cracks in metallic materials. The applicability of the model to the small fatigue crack growth behavior of four engineering alloys was examined. Small fatigue cracks were initiated and propagated, in a controlled manner, from micronotches fabricated by femtosecond pulsed laser micromachining. The results suggest that a methodology consisting of crack-tip damage accumulation and fracture provides a common framework to estimate the fatigue crack propagation lifetime of structural materials.

  5. Deep-water riser fatigue monitoring systems based on acoustic telemetry

    NASA Astrophysics Data System (ADS)

    Li, Baojun; Wang, Haiyan; Shen, Xiaohong; Yan, Yongsheng; Yang, Fuzhou; Hua, Fei

    2014-12-01

    Marine risers play a key role in the deep and ultra-deep water oil and gas production. The vortex-induced vibration (VIV) of marine risers constitutes an important problem in deep water oil exploration and production. VIV will result in high rates of structural failure of marine riser due to fatigue damage accumulation and diminishes the riser fatigue life. In-service monitoring or full scale testing is essential to improve our understanding of VIV response and enhance our ability to predict fatigue damage. One marine riser fatigue acoustic telemetry scheme is proposed and an engineering prototype machine has been developed to monitor deep and ultra-deep water risers' fatigue and failure that can diminish the riser fatigue life and lead to economic losses and eco-catastrophe. Many breakthroughs and innovation have been achieved in the process of developing an engineering prototype machine. Sea trials were done on the 6th generation deep-water drilling platform HYSY-981 in the South China Sea. The inclination monitoring results show that the marine riser fatigue acoustic telemetry scheme is feasible and reliable and the engineering prototype machine meets the design criterion and can match the requirements of deep and ultra-deep water riser fatigue monitoring. The rich experience and field data gained in the sea trial which provide much technical support for optimization in the engineering prototype machine in the future.

  6. The analysis of fatigue crack growth mechanism and oxidation and fatigue life at elevated temperatures

    NASA Technical Reports Server (NTRS)

    Liu, H. W.

    1988-01-01

    Two quantitative models based on experimentally observed fatigue damage processes have been made: (1) a model of low cycle fatigue life based on fatigue crack growth under general-yielding cyclic loading; and (2) a model of accelerated fatigue crack growth at elevated temperatures based on grain boundary oxidation. These two quantitative models agree very well with the experimental observations.

  7. A computer program for cyclic plasticity and structural fatigue analysis

    NASA Technical Reports Server (NTRS)

    Kalev, I.

    1980-01-01

    A computerized tool for the analysis of time independent cyclic plasticity structural response, life to crack initiation prediction, and crack growth rate prediction for metallic materials is described. Three analytical items are combined: the finite element method with its associated numerical techniques for idealization of the structural component, cyclic plasticity models for idealization of the material behavior, and damage accumulation criteria for the fatigue failure.

  8. Fatigue monitoring in Nuclear Power Plants

    SciTech Connect

    Ware, A.G.; Shah, V.N.

    1995-04-01

    This paper summarizes fatigue monitoring methods and surveys their application in the nuclear power industry. The paper is based on a review of the technical literature. Two main reasons for fatigue monitoring are more frequent occurrence of some transients than that assumed in the fatigue design analysis and the discovery of stressors that were not included in the fatigue design analysis but may cause significant fatigue damage at some locations. One fatigue monitoring method involves use of plant operating data and procedures to update the fatigue usage. Another method involves monitoring of plant operating parameters using existing, or if needed, supplementary plant instrumentation for online computation of fatigue usage. Use of fatigue monitoring has better defined the operational transients. Most operational transients have been found less severe and fewer in numbers than anticipated in the design fatigue analysis. Use of fatigue monitoring has assisted in quantifying newly discovered stressors and has helped in detecting the presence of thermal stratification of unsuspected locations.

  9. ROS accumulation and oxidative damage to cell structures in Saccharomyces cerevisiae wine strains during fermentation of high-sugar-containing medium.

    PubMed

    Landolfo, Sara; Politi, Huguette; Angelozzi, Daniele; Mannazzu, Ilaria

    2008-06-01

    To further elucidate the impact of fermentative stress on Saccharomyces cerevisiae wine strains, we have here evaluated markers of oxidative stress, oxidative damage and antioxidant response in four oenological strains of S. cerevisiae, relating these to membrane integrity, ethanol production and cell viability during fermentation in high-sugar-containing medium. The cells were sampled at different fermentation stages and analysed by flow cytometry to evaluate membrane integrity and accumulation of reactive oxygen species (ROS). At the same time, catalase and superoxide dismutase activities, trehalose accumulation, and protein carbonylation and degradation were measured. The results indicate that the stress conditions occurring during hypoxic fermentation in high-sugar-containing medium result in the production of ROS and trigger an antioxidant response. This involves superoxide dismutase and trehalose for the protection of cell structures from oxidative damage, and protein catabolism for the removal of damaged proteins. Cell viability, membrane integrity and ethanol production depend on the extent of oxidative damage to cellular components. This is, in turn, related to the 'fitness' of each strain, which depends on the contribution of individual cells to ROS accumulation and scavenging. These findings highlight that the differences in individual cell resistances to ROS contribute to the persistence of wine strains during growth under unfavourable culture conditions, and they provide further insights into our understanding of yeast behaviour during industrial fermentation.

  10. ATM-dependent E2F1 accumulation in the nucleolus is an indicator of ribosomal stress in early response to DNA damage.

    PubMed

    Jin, Ya-Qiong; An, Guo-Shun; Ni, Ju-Hua; Li, Shu-Yan; Jia, Hong-Ti

    2014-01-01

    The nucleolus plays a major role in ribosome biogenesis. Most genotoxic agents disrupt nucleolar structure and function, which results in the stabilization/activation of p53, inducing cell cycle arrest or apoptosis. Likewise, transcription factor E2F1 as a DNA damage responsive protein also plays roles in cell cycle arrest, DNA repair, or apoptosis in response to DNA damage through transcriptional response and protein-protein interaction. Furthermore, E2F1 is known to be involved in regulating rRNA transcription. However, how E2F1 displays in coordinating DNA damage and nucleolar stress is unclear. In this study, we demonstrate that ATM-dependent E2F1 accumulation in the nucleolus is a characteristic feature of nucleolar stress in early response to DNA damage. We found that at the early stage of DNA damage, E2F1 accumulation in the nucleolus was an ATM-dependent and a common event in p53-suficient and -deficient cells. Increased nucleolar E2F1 was sequestered by the nucleolar protein p14ARF, which repressed E2F1-dependent rRNA transcription initiation, and was coupled with S phase. Our data indicate that early accumulation of E2F1 in the nucleolus is an indicator for nucleolar stress and a component of ATM pathway, which presumably buffers elevation of E2F1 in the nucleoplasm and coordinates the diversifying mechanisms of E2F1 acts in cell cycle progression and apoptosis in early response to DNA damage.

  11. Thermal fatigue durability for advanced propulsion materials

    NASA Technical Reports Server (NTRS)

    Halford, Gary R.

    1989-01-01

    A review is presented of thermal and thermomechanical fatigue (TMF) crack initiation life prediction and cyclic constitutive modeling efforts sponsored recently by the NASA Lewis Research Center in support of advanced aeronautical propulsion research. A brief description is provided of the more significant material durability models that were created to describe TMF fatigue resistance of both isotropic and anisotropic superalloys, with and without oxidation resistant coatings. The two most significant crack initiation models are the cyclic damage accumulation model and the total strain version of strainrange partitioning. Unified viscoplastic cyclic constitutive models are also described. A troika of industry, university, and government research organizations contributed to the generation of these analytic models. Based upon current capabilities and established requirements, an attempt is made to project which TMF research activities most likely will impact future generation propulsion systems.

  12. Creep fatigue life prediction for engine hot section materials (isotropic)

    NASA Technical Reports Server (NTRS)

    Moreno, Vito; Nissley, David; Lin, Li-Sen Jim

    1985-01-01

    The first two years of a two-phase program aimed at improving the high temperature crack initiation life prediction technology for gas turbine hot section components are discussed. In Phase 1 (baseline) effort, low cycle fatigue (LCF) models, using a data base generated for a cast nickel base gas turbine hot section alloy (B1900+Hf), were evaluated for their ability to predict the crack initiation life for relevant creep-fatigue loading conditions and to define data required for determination of model constants. The variables included strain range and rate, mean strain, strain hold times and temperature. None of the models predicted all of the life trends within reasonable data requirements. A Cycle Damage Accumulation (CDA) was therefore developed which follows an exhaustion of material ductility approach. Material ductility is estimated based on observed similarities of deformation structure between fatigue, tensile and creep tests. The cycle damage function is based on total strain range, maximum stress and stress amplitude and includes both time independent and time dependent components. The CDA model accurately predicts all of the trends in creep-fatigue life with loading conditions. In addition, all of the CDA model constants are determinable from rapid cycle, fully reversed fatigue tests and monotonic tensile and/or creep data.

  13. Fault core and damage zone fracture attributes vary along strike owing to interaction of fracture growth, quartz accumulation, and differing sandstone composition

    NASA Astrophysics Data System (ADS)

    Laubach, S. E.; Eichhubl, P.; Hargrove, P.; Ellis, M. A.; Hooker, J. N.

    2014-11-01

    Small, meter-to decimeter-displacement oblique-slip faults cut latest Precambrian lithic arkose to feldspathic litharenite and Cambrian quartz arenite sandstones in NW Scotland. Despite common slip and thermal histories during faulting, the two sandstone units have different fault-core and damage-zone attributes, including fracture length and aperture distributions, and location of quartz deposits. Fault cores are narrow (less than 1 m), low-porosity cataclasite in lithic arkose/feldspathic litharenites. Damage zone-parallel opening-mode fractures are long (meters or more) with narrow ranges of lengths and apertures, are mostly isolated, have sparse quartz cement, and are open. In contrast, quartz arenites, despite abundant quartz cement, have fault cores that contain porous breccia and dense, striated slip zones. Damage-zone fractures have lengths ranging from meters to centimeters or less, but with distributions skewed to short fractures, and have power-law aperture distributions. Owing to extensive quartz cement, they tend to be sealed. These attributes reflect inhibited authigenic quartz accumulation on feldspar and lithic grains, which are unfavorable precipitation substrates, and favored accumulation on detrital quartz. In quartz breccia, macropores >0.04 mm wide persist where surrounded by slow-growing euhedral quartz. Differences in quartz occurrence and size distributions are compatible with the hypothesis that cement deposits modify the probability of fracture reactivation. Existing fractures readily reactivate in focused growth where quartz accumulation is low and porosity high. Only some existing, partly cemented fractures reactivate and some deformation is manifest in new fracture formation in partitioned growth where quartz accumulation is high. Consequences include along-strike differences in permeability and locus of fluid flow between cores and damage zones and fault strength.

  14. Computer simulation of fatigue under diametrical compression

    SciTech Connect

    Carmona, H. A.; Kun, F.; Andrade, J. S. Jr.; Herrmann, H. J.

    2007-04-15

    We study the fatigue fracture of disordered materials by means of computer simulations of a discrete element model. We extend a two-dimensional fracture model to capture the microscopic mechanisms relevant for fatigue and we simulate the diametric compression of a disc shape specimen under a constant external force. The model allows us to follow the development of the fracture process on the macrolevel and microlevel varying the relative influence of the mechanisms of damage accumulation over the load history and healing of microcracks. As a specific example we consider recent experimental results on the fatigue fracture of asphalt. Our numerical simulations show that for intermediate applied loads the lifetime of the specimen presents a power law behavior. Under the effect of healing, more prominent for small loads compared to the tensile strength of the material, the lifetime of the sample increases and a fatigue limit emerges below which no macroscopic failure occurs. The numerical results are in a good qualitative agreement with the experimental findings.

  15. NASA GRC Fatigue Crack Initiation Life Prediction Models

    NASA Technical Reports Server (NTRS)

    Arya, Vinod K.; Halford, Gary R.

    2002-01-01

    Metal fatigue has plagued structural components for centuries, and it remains a critical durability issue in today's aerospace hardware. This is true despite vastly improved and advanced materials, increased mechanistic understanding, and development of accurate structural analysis and advanced fatigue life prediction tools. Each advance is quickly taken advantage of to produce safer, more reliable, more cost effective, and better performing products. In other words, as the envelope is expanded, components are then designed to operate just as close to the newly expanded envelope as they were to the initial one. The problem is perennial. The economic importance of addressing structural durability issues early in the design process is emphasized. Tradeoffs with performance, cost, and legislated restrictions are pointed out. Several aspects of structural durability of advanced systems, advanced materials and advanced fatigue life prediction methods are presented. Specific items include the basic elements of durability analysis, conventional designs, barriers to be overcome for advanced systems, high-temperature life prediction for both creep-fatigue and thermomechanical fatigue, mean stress effects, multiaxial stress-strain states, and cumulative fatigue damage accumulation assessment.

  16. A Primer In Advanced Fatigue Life Prediction Methods

    NASA Technical Reports Server (NTRS)

    Halford, Gary R.

    2000-01-01

    Metal fatigue has plagued structural components for centuries, and it remains a critical durability issue in today's aerospace hardware. This is true despite vastly improved and advanced materials, increased mechanistic understanding, and development of accurate structural analysis and advanced fatigue life prediction tools. Each advance is quickly taken advantage of to produce safer, more reliable more cost effective, and better performing products. In other words, as the envelop is expanded, components are then designed to operate just as close to the newly expanded envelop as they were to the initial one. The problem is perennial. The economic importance of addressing structural durability issues early in the design process is emphasized. Tradeoffs with performance, cost, and legislated restrictions are pointed out. Several aspects of structural durability of advanced systems, advanced materials and advanced fatigue life prediction methods are presented. Specific items include the basic elements of durability analysis, conventional designs, barriers to be overcome for advanced systems, high-temperature life prediction for both creep-fatigue and thermomechanical fatigue, mean stress effects, multiaxial stress-strain states, and cumulative fatigue damage accumulation assessment.

  17. Environmental Effects on Graphite-Epoxy Fatigue Properties

    NASA Technical Reports Server (NTRS)

    Sumsion, H. T.

    1976-01-01

    Effects of torsional and flexural fatigue on the long-time Integrity of advanced graphite-epoxy structural composites have been investigated. Torsional fatigue tests were run at stress ratios of R = 0 (zero to maximum, repeated) and R = -1 (zero mean stress) on unidirectional, angleply, and woven graphite fiber materials in air and water at room temperature and at 74 C. Flexural fatigue tests (four-point bending) with R = -1 were run in air and water at room temperature, and with R = 0 in air. Results show that, in torsional cycling, both water environment and higher test temperature contribute to significant degradation of torsional stiffness. The degradation of stiffness from torsional stress cycling was observed to be much greater with R = -1 than with simple R = 0 cycling. The effect of environment also is greater in the fully reversed cycling. Flexural fatigue results on +/- 30 deg material show a large fatigue effect, with fatigue limits of less than 50% and 30% of the static failure strength for specimens tested under stress ratios of R = 0 and R = -1, respectively. Compliance measurements indicate that the final failures are preceded by damage initiation and accumulation, which begins at about 1% of the specimen life.

  18. A procedure for utilization of a damage-dependent constitutive model for laminated composites

    NASA Technical Reports Server (NTRS)

    Lo, David C.; Allen, David H.; Harris, Charles E.

    1992-01-01

    Described here is the procedure for utilizing a damage constitutive model to predict progressive damage growth in laminated composites. In this model, the effects of the internal damage are represented by strain-like second order tensorial damage variables and enter the analysis through damage dependent ply level and laminate level constitutive equations. The growth of matrix cracks due to fatigue loading is predicted by an experimentally based damage evolutionary relationship. This model is incorporated into a computer code called FLAMSTR. This code is capable of predicting the constitutive response and matrix crack damage accumulation in fatigue loaded laminated composites. The structure and usage of FLAMSTR are presented along with sample input and output files to assist the code user. As an example problem, an analysis of crossply laminates subjected to two stage fatigue loading was conducted and the resulting damage accumulation and stress redistribution were examined to determine the effect of variations in fatigue load amplitude applied during the first stage of the load history. It was found that the model predicts a significant loading history effect on damage evolution.

  19. A study of elevated temperature testing techniques for the fatigue behavior of PMCS: Application to T650-35/AMB21

    NASA Technical Reports Server (NTRS)

    Gyekenyesi, Andrew L.; Gastelli, Michael G.; Ellis, John R.; Burke, Christopher S.

    1995-01-01

    An experimental study was conducted to investigate the mechanical behavior of a T650-35/AMB21 eight-harness satin weave polymer composite system. Emphasis was placed on the development and refinement of techniques used in elevated temperature uniaxial PMC testing. Issues such as specimen design, gripping, strain measurement, and temperature control and measurement were addressed. Quasi-static tensile and fatigue properties (R(sub sigma) = 0.1) were examined at room and elevated temperatures. Stiffness degradation and strain accumulation during fatigue cycling were recorded to monitor damage progression and provide insight for future analytical modeling efforts. Accomplishments included an untabbed dog-bone specimen design which consistently failed in the gage section, accurate temperature control and assessment, and continuous in-situ strain measurement capability during fatigue loading at elevated temperatures. Finally, strain accumulation and stiffness degradation during fatigue cycling appeared to be good indicators of damage progression.

  20. Reduced Order Methods for Prediction of Thermal-Acoustic Fatigue

    NASA Technical Reports Server (NTRS)

    Przekop, A.; Rizzi, S. A.

    2004-01-01

    The goal of this investigation is to assess the quality of high-cycle-fatigue life estimation via a reduced order method, for structures undergoing random nonlinear vibrations in a presence of thermal loading. Modal reduction is performed with several different suites of basis functions. After numerically solving the reduced order system equations of motion, the physical displacement time history is obtained by an inverse transformation and stresses are recovered. Stress ranges obtained through the rainflow counting procedure are used in a linear damage accumulation method to yield fatigue estimates. Fatigue life estimates obtained using various basis functions in the reduced order method are compared with those obtained from numerical simulation in physical degrees-of-freedom.

  1. Exceptional high fatigue strength in Cu-15at.%Al alloy with moderate grain size

    PubMed Central

    Liu, Rui; Tian, Yanzhong; Zhang, Zhenjun; An, Xianghai; Zhang, Peng; Zhang, Zhefeng

    2016-01-01

    It is commonly proposed that the fatigue strength can be enhanced by increasing the tensile strength, but this conclusion needs to be reconsidered according to our study. Here a recrystallized α-Cu-15at.%Al alloy with moderate grain size of 0.62 μm was fabricated by cold rolling and annealing, and this alloy achieved exceptional high fatigue strength of 280 MPa at 107 cycles. This value is much higher than the fatigue strength of 200 MPa for the nano-crystalline counterpart (0.04 μm in grain size) despite its higher tensile strength. The remarkable improvement of fatigue strength should be mainly attributed to the microstructure optimization, which helps achieve the reduction of initial damage and the dispersion of accumulated damage. A new strategy of “damage reduction” was then proposed for fatigue strength improvement, to supplement the former strengthening principle. The methods and strategies summarized in this work offer a general pathway for further improvement of fatigue strength, in order to ensure the long-term safety of structural materials. PMID:27264347

  2. Exceptional high fatigue strength in Cu-15at.%Al alloy with moderate grain size

    NASA Astrophysics Data System (ADS)

    Liu, Rui; Tian, Yanzhong; Zhang, Zhenjun; An, Xianghai; Zhang, Peng; Zhang, Zhefeng

    2016-06-01

    It is commonly proposed that the fatigue strength can be enhanced by increasing the tensile strength, but this conclusion needs to be reconsidered according to our study. Here a recrystallized α-Cu-15at.%Al alloy with moderate grain size of 0.62 μm was fabricated by cold rolling and annealing, and this alloy achieved exceptional high fatigue strength of 280 MPa at 107 cycles. This value is much higher than the fatigue strength of 200 MPa for the nano-crystalline counterpart (0.04 μm in grain size) despite its higher tensile strength. The remarkable improvement of fatigue strength should be mainly attributed to the microstructure optimization, which helps achieve the reduction of initial damage and the dispersion of accumulated damage. A new strategy of “damage reduction” was then proposed for fatigue strength improvement, to supplement the former strengthening principle. The methods and strategies summarized in this work offer a general pathway for further improvement of fatigue strength, in order to ensure the long-term safety of structural materials.

  3. Exceptional high fatigue strength in Cu-15at.%Al alloy with moderate grain size.

    PubMed

    Liu, Rui; Tian, Yanzhong; Zhang, Zhenjun; An, Xianghai; Zhang, Peng; Zhang, Zhefeng

    2016-01-01

    It is commonly proposed that the fatigue strength can be enhanced by increasing the tensile strength, but this conclusion needs to be reconsidered according to our study. Here a recrystallized α-Cu-15at.%Al alloy with moderate grain size of 0.62 μm was fabricated by cold rolling and annealing, and this alloy achieved exceptional high fatigue strength of 280 MPa at 10(7) cycles. This value is much higher than the fatigue strength of 200 MPa for the nano-crystalline counterpart (0.04 μm in grain size) despite its higher tensile strength. The remarkable improvement of fatigue strength should be mainly attributed to the microstructure optimization, which helps achieve the reduction of initial damage and the dispersion of accumulated damage. A new strategy of "damage reduction" was then proposed for fatigue strength improvement, to supplement the former strengthening principle. The methods and strategies summarized in this work offer a general pathway for further improvement of fatigue strength, in order to ensure the long-term safety of structural materials. PMID:27264347

  4. The free-radical damage theory: Accumulating evidence against a simple link of oxidative stress to ageing and lifespan.

    PubMed

    Speakman, John R; Selman, Colin

    2011-04-01

    Recent work on a small European cave salamander (Proteus anguinus) has revealed that it has exceptional longevity, yet it appears to have unexceptional defences against oxidative damage. This paper comes at the end of a string of other studies that are calling into question the free-radical damage theory of ageing. This theory rose to prominence in the 1990s as the dominant theory for why we age and die. Despite substantial correlative evidence to support it, studies in the last five years have raised doubts over its importance. In particular, these include studies of mice with the major antioxidant genes knocked out (both singly and in combination), which show the expected elevation in oxidative damage but no impact on lifespan. Combined, these findings raise fundamental questions over whether the free-radical damage theory remains useful for understanding the ageing process, and variation in lifespan and life histories.

  5. The accumulation of brain water-free sodium is associated with ischemic damage independent of the blood pressure in female rats.

    PubMed

    Sumiyoshi, Manabu; Kitazato, Keiko T; Yagi, Kenji; Miyamoto, Takeshi; Kurashiki, Yoshitaka; Matsushita, Nobuhisa; Kinouchi, Tomoya; Kuwayama, Kazuyuki; Satomi, Junichiro; Nagahiro, Shinji

    2015-08-01

    Estrogen deficiency worsens ischemic stroke outcomes. In ovariectomized (OVX(+)) rats fed a high-salt diet (HSD), an increase in the body Na(+)/water ratio, which characterizes water-free Na(+) accumulation, was associated with detrimental vascular effects independent of the blood pressure (BP). We hypothesized that an increase in brain water-free Na(+) accumulation is associated with ischemic brain damage in OVX(+)/HSD rats. To test our hypothesis we divided female Wistar rats into 4 groups, OVX(+) and OVX(-) rats fed HSD or a normal diet (ND), and subjected them to transient cerebral ischemia. The brain Na(+)/water ratio was increased even in OVX(+)/ND rats and augmented in OVX(+)/HSD rats. The increase in the brain Na(+)/water ratio was positively correlated with expansion of the cortical infarct volume without affecting the BP. Interestingly, OVX(+) was associated with the decreased expression of ATP1α3, a subtype of the Na(+) efflux pump. HSD increased the expression of brain Na(+) influx-related molecules and the mineralocorticoid receptor (MR). The pretreatment of OVX(+)/HSD rats with the MR antagonist eplerenone reduced brain water-free Na(+) accumulation, up-regulated ATP1α3, down-regulated MR, and reduced the cortical infarct volume. Our findings show that the increase in the brain Na(+)/water ratio elicited by estrogen deficiency or HSD is associated with ischemic brain damage BP-independently, suggesting the importance of regulating the accumulation of brain water-free Na(+). The up-regulation of ATP1α3 and the down-regulation of MR may provide a promising therapeutic strategy to attenuate ischemic brain damage in postmenopausal women.

  6. A Novel Approach to Rotorcraft Damage Tolerance

    NASA Technical Reports Server (NTRS)

    Forth, Scott C.; Everett, Richard A.; Newman, John A.

    2002-01-01

    Damage-tolerance methodology is positioned to replace safe-life methodologies for designing rotorcraft structures. The argument for implementing a damage-tolerance method comes from the fundamental fact that rotorcraft structures typically fail by fatigue cracking. Therefore, if technology permits prediction of fatigue-crack growth in structures, a damage-tolerance method should deliver the most accurate prediction of component life. Implementing damage-tolerance (DT) into high-cycle-fatigue (HCF) components will require a shift from traditional DT methods that rely on detecting an initial flaw with nondestructive inspection (NDI) methods. The rapid accumulation of cycles in a HCF component will result in a design based on a traditional DT method that is either impractical because of frequent inspections, or because the design will be too heavy to operate efficiently. Furthermore, once a HCF component develops a detectable propagating crack, the remaining fatigue life is short, sometimes less than one flight hour, which does not leave sufficient time for inspection. Therefore, designing a HCF component will require basing the life analysis on an initial flaw that is undetectable with current NDI technology.

  7. Modulation of gemcitabine accumulation by DNA-damaging agents: mechanisms and specificity in an in vitro model.

    PubMed

    Metharom, Ekkaphon; Galettis, Peter; Manners, Susan; Links, Matthew

    2010-09-01

    Self-potentiation of the intracellular accumulation of gemcitabine accumulation occurs with repeated administration. Understanding the mechanism of this phenomena and its occurrence with other drugs is important for rational dosing of gemcitabine and design of gemcitabine combinations. The HCT116 cell line was used as a model of the in vivo findings to examine the effect of repeated gemcitabine exposure. HPLC analysis revealed a 10-fold increase in gemcitabine-triphosphate accumulation upon repeated gemcitabine exposure. The induction of accumulation was not associated with any changes in the dCK mRNA level. Comparable increases in gemcitabine-triphosphate were seen when the cells were pre-incubated with cytarabine and cisplatin. A lesser increase and no increase in GEM-TP were seen with oxaliplatin and 5'-azacytidine, respectively. In this model, induction of gemcitabine accumulation is likely to be mediated by post translational modification of dCK. The reduced effect of oxaliplatin compared to cisplatin is worthy of further study.

  8. Stiffness reductions during tensile fatigue testing of graphite/epoxy angle-ply laminates

    NASA Technical Reports Server (NTRS)

    Odom, E. M.; Adams, D. F.

    1983-01-01

    The monitoring of modulus decay during fatigue testing as an indicator of damage accumulation and incipient failure has frequently been postulated. The present study was designed to generate tensile fatigue data under carefully controlled test conditions. A computerized data acquisition system was used to permit the measurement of dynamic modulus without interrupting the fatigue cycling. Two different 8-ply laminate configurations, viz, (+ or - 45)2s and (+ or - 67.5)2s, of a T300/5208 graphite/epoxy composite were tested. The (+ or 45)2s laminate did exhibit some modulus decay, although there was no well-defined correlation with applied stress level or number of cycles. The (+ or - 67.5)2s laminate did not exhibit any measurable modulus decay. Secondary effects observed included a slight recovery of the modulus decay after a test interruption, and a significant viscoelastic (creep) response of the (+ or - 45)2s laminate during fatigue testing.

  9. Impairment of aldehyde dehydrogenase 2 increases accumulation of acetaldehyde-derived DNA damage in the esophagus after ethanol ingestion

    PubMed Central

    Yukawa, Yoshiyuki; Ohashi, Shinya; Amanuma, Yusuke; Nakai, Yukie; Tsurumaki, Mihoko; Kikuchi, Osamu; Miyamoto, Shin’ichi; Oyama, Tsunehiro; Kawamoto, Toshihiro; Chiba, Tsutomu; Matsuda, Tomonari; Muto, Manabu

    2014-01-01

    Ethanol and its metabolite, acetaldehyde, are the definite carcinogens for esophageal squamous cell carcinoma (ESCC), and reduced catalytic activity of aldehyde dehydrogenase 2 (ALDH2), which detoxifies acetaldehyde, increases the risk for ESCC. However, it remains unknown whether the ALDH2 genotype influences the level of acetaldehyde-derived DNA damage in the esophagus after ethanol ingestion. In the present study, we administered ethanol orally or intraperitoneally to Aldh2-knockout and control mice, and we quantified the level of acetaldehyde-derived DNA damage, especially N2-ethylidene-2’-deoxyguanosine (N2-ethylidene-dG), in the esophagus. In the model of oral ethanol administration, the esophageal N2-ethylidene-dG level was significantly higher in Aldh2-knockout mice compared with control mice. Similarly, in the model of intraperitoneal ethanol administration, in which the esophagus is not exposed directly to the alcohol solution, the esophageal N2-ethylidene-dG level was also elevated in Aldh2-knockout mice. This result indicates that circulating ethanol-derived acetaldehyde causes esophageal DNA damage, and that the extent of damage is influenced by knockout of Aldh2. Taken together, our findings strongly suggest the importance of acetaldehyde-derived DNA damage which is induced in the esophagus of individuals with ALDH2 gene impairment. This provides a physiological basis for understanding alcohol-related esophageal carcinogenesis. PMID:24959382

  10. Impairment of aldehyde dehydrogenase 2 increases accumulation of acetaldehyde-derived DNA damage in the esophagus after ethanol ingestion.

    PubMed

    Yukawa, Yoshiyuki; Ohashi, Shinya; Amanuma, Yusuke; Nakai, Yukie; Tsurumaki, Mihoko; Kikuchi, Osamu; Miyamoto, Shin'ichi; Oyama, Tsunehiro; Kawamoto, Toshihiro; Chiba, Tsutomu; Matsuda, Tomonari; Muto, Manabu

    2014-01-01

    Ethanol and its metabolite, acetaldehyde, are the definite carcinogens for esophageal squamous cell carcinoma (ESCC), and reduced catalytic activity of aldehyde dehydrogenase 2 (ALDH2), which detoxifies acetaldehyde, increases the risk for ESCC. However, it remains unknown whether the ALDH2 genotype influences the level of acetaldehyde-derived DNA damage in the esophagus after ethanol ingestion. In the present study, we administered ethanol orally or intraperitoneally to Aldh2-knockout and control mice, and we quantified the level of acetaldehyde-derived DNA damage, especially N(2) -ethylidene-2'-deoxyguanosine (N(2) -ethylidene-dG), in the esophagus. In the model of oral ethanol administration, the esophageal N(2) -ethylidene-dG level was significantly higher in Aldh2-knockout mice compared with control mice. Similarly, in the model of intraperitoneal ethanol administration, in which the esophagus is not exposed directly to the alcohol solution, the esophageal N(2) -ethylidene-dG level was also elevated in Aldh2-knockout mice. This result indicates that circulating ethanol-derived acetaldehyde causes esophageal DNA damage, and that the extent of damage is influenced by knockout of Aldh2. Taken together, our findings strongly suggest the importance of acetaldehyde-derived DNA damage which is induced in the esophagus of individuals with ALDH2 gene impairment. This provides a physiological basis for understanding alcohol-related esophageal carcinogenesis. PMID:24959382

  11. Significant accumulation of persistent organic pollutants and dysregulation in multiple DNA damage repair pathways in the electronic-waste-exposed populations

    SciTech Connect

    He, Xiaobo; Jing, Yaqing; Wang, Jianhai; Li, Keqiu; Yang, Qiaoyun; Zhao, Yuxia; Li, Ran; Ge, Jie; Qiu, Xinghua; Li, Guang

    2015-02-15

    Electronic waste (e-waste) has created a worldwide environmental and health problem, by generating a diverse group of hazardous compounds such as persistent organic pollutants (POPs). Our previous studies demonstrated that populations from e-waste exposed region have a significantly higher level of chromosomal aberrancy and incidence of DNA damage. In this study, we further demonstrated that various POPs persisted at a significantly higher concentration in the exposed group than those in the unexposed group. The level of reactive oxygen species and micronucleus rate were also significantly elevated in the exposed group. RNA sequencing analysis revealed 31 genes in DNA damage responses and repair pathways that were differentially expressed between the two groups (Log 2 ratio >1 or <−1). Our data demonstrated that both females and males of the exposed group have activated a series of DNA damage response genes; however many important DNA repair pathways have been dysregulated. Expressions of NEIL1/3 and RPA3, which are critical in initiating base pair and nucleotide excision repairs respectively, have been downregulated in both females and males of the exposed group. In contrast, expression of RNF8, an E3 ligase involved in an error prone non-homologous end joining repair for DNA double strand break, was upregulated in both genders of the exposed group. The other genes appeared to be differentially expressed only when the males or females of the two groups were compared respectively. Importantly, the expression of cell cycle regulatory gene CDC25A that has been implicated in multiple kinds of malignant transformation was significantly upregulated among the exposed males while downregulated among the exposed females. In conclusion, our studies have demonstrated significant correlations between e-waste disposing and POPs accumulation, DNA lesions and dysregulation of multiple DNA damage repair mechanisms in the residents of the e-waste exposed region. - Highlights:

  12. Prevention of DNA damage by L-carnitine induced by metabolites accumulated in maple syrup urine disease in human peripheral leukocytes in vitro.

    PubMed

    Mescka, Caroline Paula; Wayhs, Carlos Alberto Yasin; Guerreiro, Gilian; Manfredini, Vanusa; Dutra-Filho, Carlos Severo; Vargas, Carmen Regla

    2014-09-15

    Maple syrup urine disease (MSUD) is an inherited aminoacidopathy caused by a deficiency in branched-chain α-keto acid dehydrogenase complex activity that leads to the accumulation of the branched-chain amino acids (BCAAs) leucine (Leu), isoleucine, and valine and their respective α-keto-acids, α-ketoisocaproic acid (KIC), α keto-β-methylvaleric acid, and α-ketoisovaleric acid. The major clinical features presented by MSUD patients include ketoacidosis, failure to thrive, poor feeding, apnea, ataxia, seizures, coma, psychomotor delay, and mental retardation; however, the pathophysiology of this disease is poorly understood. MSUD treatment consists of a low protein diet supplemented with a mixture containing micronutrients and essential amino acids but excluding BCAAs. Studies have shown that oxidative stress may be involved in the neuropathology of MSUD, with the existence of lipid and protein oxidative damage in affected patients. In recent years, studies have demonstrated the antioxidant role of L-carnitine (L-Car), which plays a central function in cellular energy metabolism and for which MSUD patients have a deficiency. In this work, we investigated the in vitro effect of Leu and KIC in the presence or absence of L-Car on DNA damage in peripheral whole blood leukocytes using the alkaline comet assay with silver staining and visual scoring. Leu and KIC resulted in a DNA damage index that was significantly higher than that of the control group, and L-Car was able to significantly prevent this damage, mainly that due to KIC. PMID:25046137

  13. SMCHD1 accumulates at DNA damage sites and facilitates the repair of DNA double-strand breaks

    PubMed Central

    Coker, Heather; Brockdorff, Neil

    2014-01-01

    ABSTRACT SMCHD1 is a structural maintenance of chromosomes (SMC) family protein involved in epigenetic gene silencing and chromosome organisation on the female inactive X chromosome and at a limited number of autosomal loci. Here, we demonstrate that SMCHD1 also has a role in DNA repair of double-strand breaks; SMCHD1 is recruited to sites of laser micro-irradiated damage along with other DNA repair factors, including Ku80 (also known as XRCC5 in mammals) and RAD51. Cells deficient in SMCHD1 show evidence of decreased efficiency of repair and cell viability after DNA damage. We suggest that SMCHD1 responds to DNA double-strand breaks in a manner that is likely to involve its ability to alter chromatin states to facilitate DNA repair. PMID:24790221

  14. Gear Damage Detection Using Oil Debris Analysis

    NASA Technical Reports Server (NTRS)

    Dempsey, Paula J.

    2001-01-01

    The purpose of this paper was to verify, when using an oil debris sensor, that accumulated mass predicts gear pitting damage and to identify a method to set threshold limits for damaged gears. Oil debris data was collected from 8 experiments with no damage and 8 with pitting damage in the NASA Glenn Spur Gear Fatigue Rig. Oil debris feature analysis was performed on this data. Video images of damage progression were also collected from 6 of the experiments with pitting damage. During each test, data from an oil debris sensor was monitored and recorded for the occurrence of pitting damage. The data measured from the oil debris sensor during experiments with damage and with no damage was used to identify membership functions to build a simple fuzzy logic model. Using fuzzy logic techniques and the oil debris data, threshold limits were defined that discriminate between stages of pitting wear. Results indicate accumulated mass combined with fuzzy logic analysis techniques is a good predictor of pitting damage on spur gears.

  15. Thermomechanical Fatigue of Ductile Cast Iron and Its Life Prediction

    NASA Astrophysics Data System (ADS)

    Wu, Xijia; Quan, Guangchun; MacNeil, Ryan; Zhang, Zhong; Liu, Xiaoyang; Sloss, Clayton

    2015-06-01

    Thermomechanical fatigue (TMF) behaviors of ductile cast iron (DCI) were investigated under out-of-phase (OP), in-phase (IP), and constrained strain-control conditions with temperature hold in various temperature ranges: 573 K to 1073 K, 723 K to 1073 K, and 433 K to 873 K (300 °C to 800 °C, 450 °C to 800 °C, and 160 °C to 600 °C). The integrated creep-fatigue theory (ICFT) model was incorporated into the finite element method to simulate the hysteresis behavior and predict the TMF life of DCI under those test conditions. With the consideration of four deformation/damage mechanisms: (i) plasticity-induced fatigue, (ii) intergranular embrittlement, (iii) creep, and (iv) oxidation, as revealed from the previous study on low cycle fatigue of the material, the model delineates the contributions of these physical mechanisms in the asymmetrical hysteresis behavior and the damage accumulation process leading to final TMF failure. This study shows that the ICFT model can simulate the stress-strain response and life of DCI under complex TMF loading profiles (OP and IP, and constrained with temperature hold).

  16. Multiscale Fatigue Life Prediction for Composite Panels

    NASA Technical Reports Server (NTRS)

    Bednarcyk, Brett A.; Yarrington, Phillip W.; Arnold, Steven M.

    2012-01-01

    Fatigue life prediction capabilities have been incorporated into the HyperSizer Composite Analysis and Structural Sizing Software. The fatigue damage model is introduced at the fiber/matrix constituent scale through HyperSizer s coupling with NASA s MAC/GMC micromechanics software. This enables prediction of the micro scale damage progression throughout stiffened and sandwich panels as a function of cycles leading ultimately to simulated panel failure. The fatigue model implementation uses a cycle jumping technique such that, rather than applying a specified number of additional cycles, a specified local damage increment is specified and the number of additional cycles to reach this damage increment is calculated. In this way, the effect of stress redistribution due to damage-induced stiffness change is captured, but the fatigue simulations remain computationally efficient. The model is compared to experimental fatigue life data for two composite facesheet/foam core sandwich panels, demonstrating very good agreement.

  17. Loss of Lon1 in Arabidopsis Changes the Mitochondrial Proteome Leading to Altered Metabolite Profiles and Growth Retardation without an Accumulation of Oxidative Damage1[W][OA

    PubMed Central

    Solheim, Cory; Li, Lei; Hatzopoulos, Polydefkis; Millar, A. Harvey

    2012-01-01

    Lon1 is an ATP-dependent protease and chaperone located in the mitochondrial matrix in plants. Knockout in Arabidopsis (Arabidopsis thaliana) leads to a significant growth rate deficit in both roots and shoots and lowered activity of specific mitochondrial enzymes associated with respiratory metabolism. Analysis of the mitochondrial proteomes of two lon1 mutant alleles (lon1-1 and lon1-2) with different severities of phenotypes shows a common accumulation of several stress marker chaperones and lowered abundance of Complexes I, IV, and V of OXPHOS. Certain enzymes of the tricarboxylic acid (TCA) cycle are modified or accumulated, and TCA cycle bypasses were repressed rather than induced. While whole tissue respiratory rates were unaltered in roots and shoots, TCA cycle intermediate organic acids were depleted in leaf extracts in the day in lon1-1 and in both lon mutants at night. No significant evidence of broad steady-state oxidative damage to isolated mitochondrial samples could be found, but peptides from several specific proteins were more oxidized and selected functions were more debilitated in lon1-1. Collectively, the evidence suggests that loss of Lon1 significantly modifies respiratory function and plant performance by small but broad alterations in the mitochondrial proteome gained by subtly changing steady-state protein assembly, stability, and damage of a range of components that debilitate an anaplerotic role for mitochondria in cellular carbon metabolism. PMID:22968828

  18. Fatigue characteristics of polycrystalline silicon thin-film membrane and its dependence on humidity

    NASA Astrophysics Data System (ADS)

    Tanemura, Tomoki; Yamashita, Shuichi; Wado, Hiroyuki; Takeuchi, Yukihiro; Tsuchiya, Toshiyuki; Tabata, Osamu

    2013-03-01

    This paper describes fatigue characteristics of a polycrystalline silicon thin-film membrane under different humidity evaluated by out-of-plane resonant vibration. The membrane, without the surface of sidewalls by patterning of photolithography and etching process, was applied to evaluate fatigue characteristics precisely against the changes in the surrounding humidity owing to narrower deviation in the fatigue lifetime. The membrane has 16 mm square-shaped multilayered films consisting of a 250 or 500 nm thick polysilicon film on silicon dioxide and silicon nitride underlying layers. A circular weight of 12 mm in diameter was placed at the center of the membrane to control the resonant frequency. Stress on the polysilicon film was generated by deforming the membrane oscillating the weight in the out-of-plane direction. The polysilicon film was fractured by fatigue damage accumulation under cyclic stress. The lifetime of the polysilicon membrane extended with lower relative humidity, especially at 5%RH. The results of the fatigue tests were well formulated with Weibull's statistics and Paris’ law. The dependence of fatigue characteristics on humidity has been quantitatively revealed for the first time. The crack growth rate indicated by the fatigue index decreased with the reduction in humidity, whereas the deviation of strength represented by the Weibull modulus was nearly constant against humidity.

  19. Municipal landfill leachate-induced testicular oxidative damage is associated with biometal accumulation and endocrine disruption in rats.

    PubMed

    Adedara, Isaac A; Awogbindin, Ifeoluwa O; Adesina, Adebayo A; Oyebiyi, Oluwatosin O; Lawal, Tajudeen A; Farombi, Ebenezer O

    2015-01-01

    Improper management of hazardous wastes adversely impacts the environment and the public health. The present study was aimed at investigating the influence of Olushosun municipal landfill leachate (OMLL) from Ojota in the Lagos State of Nigeria on testicular function by assessing the plasma concentrations of reproductive hormones, testicular biometal levels, and antioxidant levels as well as observing the histological alterations in testes and epididymides of rats after exposure to 0, 12.5, and 25% OMLL in drinking water for 7 days. Exposure to OMLL significantly decreased the daily fluid intake, but it resulted in testicular biometal accumulation as follows: lead > cadmium > nickel > iron > copper. Acute exposure to OMLL induced oxidative stress and increased the activities of marker enzymes of testicular function but markedly decreased the circulatory concentrations of luteinizing hormone, follicle-stimulating hormone, prolactin, testosterone, thyroid-stimulating hormone, triiodothyronine, and thyroxine. Testicular and epididymal degeneration with significant decrease in sperm quality and quantity were observed in OMLL-exposed rats. Collectively, the data presented herein indicate that exposure to OMLL-induced testicular dysfunction associated with biometal accumulation and endocrine disruption in rats. If the effects can be extrapolated to humans, OMLL may present significant health implications for individuals exposed to OMLL-contaminated substances. PMID:25179371

  20. Municipal landfill leachate-induced testicular oxidative damage is associated with biometal accumulation and endocrine disruption in rats.

    PubMed

    Adedara, Isaac A; Awogbindin, Ifeoluwa O; Adesina, Adebayo A; Oyebiyi, Oluwatosin O; Lawal, Tajudeen A; Farombi, Ebenezer O

    2015-01-01

    Improper management of hazardous wastes adversely impacts the environment and the public health. The present study was aimed at investigating the influence of Olushosun municipal landfill leachate (OMLL) from Ojota in the Lagos State of Nigeria on testicular function by assessing the plasma concentrations of reproductive hormones, testicular biometal levels, and antioxidant levels as well as observing the histological alterations in testes and epididymides of rats after exposure to 0, 12.5, and 25% OMLL in drinking water for 7 days. Exposure to OMLL significantly decreased the daily fluid intake, but it resulted in testicular biometal accumulation as follows: lead > cadmium > nickel > iron > copper. Acute exposure to OMLL induced oxidative stress and increased the activities of marker enzymes of testicular function but markedly decreased the circulatory concentrations of luteinizing hormone, follicle-stimulating hormone, prolactin, testosterone, thyroid-stimulating hormone, triiodothyronine, and thyroxine. Testicular and epididymal degeneration with significant decrease in sperm quality and quantity were observed in OMLL-exposed rats. Collectively, the data presented herein indicate that exposure to OMLL-induced testicular dysfunction associated with biometal accumulation and endocrine disruption in rats. If the effects can be extrapolated to humans, OMLL may present significant health implications for individuals exposed to OMLL-contaminated substances.

  1. Repeated-sprint ability - part I: factors contributing to fatigue.

    PubMed

    Girard, Olivier; Mendez-Villanueva, Alberto; Bishop, David

    2011-08-01

    Short-duration sprints (<10 seconds), interspersed with brief recoveries (<60 seconds), are common during most team and racket sports. Therefore, the ability to recover and to reproduce performance in subsequent sprints is probably an important fitness requirement of athletes engaged in these disciplines, and has been termed repeated-sprint ability (RSA). This review (Part I) examines how fatigue manifests during repeated-sprint exercise (RSE), and discusses the potential underpinning muscular and neural mechanisms. A subsequent companion review to this article will explain a better understanding of the training interventions that could eventually improve RSA. Using laboratory and field-based protocols, performance analyses have consistently shown that fatigue during RSE typically manifests as a decline in maximal/mean sprint speed (i.e. running) or a decrease in peak power or total work (i.e. cycling) over sprint repetitions. A consistent result among these studies is that performance decrements (i.e. fatigue) during successive bouts are inversely correlated to initial sprint performance. To date, there is no doubt that the details of the task (e.g. changes in the nature of the work/recovery bouts) alter the time course/magnitude of fatigue development during RSE (i.e. task dependency) and potentially the contribution of the underlying mechanisms. At the muscle level, limitations in energy supply, which include energy available from phosphocreatine hydrolysis, anaerobic glycolysis and oxidative metabolism, and the intramuscular accumulation of metabolic by-products, such as hydrogen ions, emerge as key factors responsible for fatigue. Although not as extensively studied, the use of surface electromyography techniques has revealed that failure to fully activate the contracting musculature and/or changes in inter-muscle recruitment strategies (i.e. neural factors) are also associated with fatigue outcomes. Pending confirmatory research, other factors such as

  2. An RNF168 fragment defective for focal accumulation at DNA damage is proficient for inhibition of homologous recombination in BRCA1 deficient cells

    PubMed Central

    Muñoz, Meilen C.; Yanez, Diana A.; Stark, Jeremy M.

    2014-01-01

    The E3 ubiquitin ligase RNF168 is a DNA damage response (DDR) factor that promotes monoubiquitination of H2A/H2AX at K13/15, facilitates recruitment of other DDR factors (e.g. 53BP1) to DNA damage, and inhibits homologous recombination (HR) in cells deficient in the tumor suppressor BRCA1. We have examined the domains of RNF168 important for these DDR events, including chromosomal HR that is induced by several nucleases (I-SceI, CAS9-WT and CAS9-D10A), since the inducing nuclease affects the relative frequency of distinct repair outcomes. We found that an N-terminal fragment of RNF168 (1-220/N221*) efficiently inhibits HR induced by each of these nucleases in BRCA1 depleted cells, and promotes recruitment of 53BP1 to DNA damage and H2AX monoubiquitination at K13/15. Each of these DDR events requires a charged residue in RNF168 (R57). Notably, RNF168-N221* fails to self-accumulate into ionizing radiation induced foci (IRIF). Furthermore, expression of RNF168 WT and N221* can significantly bypass the role of another E3 ubiquitin ligase, RNF8, for inhibition of HR in BRCA1 depleted cells, and for promotion of 53BP1 IRIF. We suggest that the ability for RNF168 to promote H2A/H2AX monoubiquitination and 53BP1 IRIF, but not RNF168 self-accumulation into IRIF, is important for inhibition of HR in BRCA1 deficient cells. PMID:24829461

  3. Suppression of Reactive Oxygen Species Accumulation in Chloroplasts Prevents Leaf Damage but Not Growth Arrest in Salt-Stressed Tobacco Plants

    PubMed Central

    Lodeyro, Anabella F.; Giró, Mariana; Poli, Hugo O.; Bettucci, Gabriel; Cortadi, Adriana; Ferri, Alejandro M.; Carrillo, Néstor

    2016-01-01

    Crop yield reduction due to salinity is a growing agronomical concern in many regions. Increased production of reactive oxygen species (ROS) in plant cells accompanies many abiotic stresses including salinity, acting as toxic and signaling molecules during plant stress responses. While ROS are generated in various cellular compartments, chloroplasts represent a main source in the light, and plastid ROS synthesis and/or elimination have been manipulated to improve stress tolerance. Transgenic tobacco plants expressing a plastid-targeted cyanobacterial flavodoxin, a flavoprotein that prevents ROS accumulation specifically in chloroplasts, displayed increased tolerance to many environmental stresses, including drought, excess irradiation, extreme temperatures and iron starvation. Surprisingly, flavodoxin expression failed to protect transgenic plants against NaCl toxicity. However, when high salt was directly applied to leaf discs, flavodoxin did increase tolerance, as reflected by preservation of chlorophylls, carotenoids and photosynthetic activities. Flavodoxin decreased salt-dependent ROS accumulation in leaf tissue from discs and whole plants, but this decline did not improve tolerance at the whole plant level. NaCl accumulation in roots, as well as increased osmotic pressure and salt-induced root damage, were not prevented by flavodoxin expression. The results indicate that ROS formed in chloroplasts have a marginal effect on plant responses during salt stress, and that sensitive targets are present in roots which are not protected by flavodoxin. PMID:27441560

  4. Suppression of Reactive Oxygen Species Accumulation in Chloroplasts Prevents Leaf Damage but Not Growth Arrest in Salt-Stressed Tobacco Plants.

    PubMed

    Lodeyro, Anabella F; Giró, Mariana; Poli, Hugo O; Bettucci, Gabriel; Cortadi, Adriana; Ferri, Alejandro M; Carrillo, Néstor

    2016-01-01

    Crop yield reduction due to salinity is a growing agronomical concern in many regions. Increased production of reactive oxygen species (ROS) in plant cells accompanies many abiotic stresses including salinity, acting as toxic and signaling molecules during plant stress responses. While ROS are generated in various cellular compartments, chloroplasts represent a main source in the light, and plastid ROS synthesis and/or elimination have been manipulated to improve stress tolerance. Transgenic tobacco plants expressing a plastid-targeted cyanobacterial flavodoxin, a flavoprotein that prevents ROS accumulation specifically in chloroplasts, displayed increased tolerance to many environmental stresses, including drought, excess irradiation, extreme temperatures and iron starvation. Surprisingly, flavodoxin expression failed to protect transgenic plants against NaCl toxicity. However, when high salt was directly applied to leaf discs, flavodoxin did increase tolerance, as reflected by preservation of chlorophylls, carotenoids and photosynthetic activities. Flavodoxin decreased salt-dependent ROS accumulation in leaf tissue from discs and whole plants, but this decline did not improve tolerance at the whole plant level. NaCl accumulation in roots, as well as increased osmotic pressure and salt-induced root damage, were not prevented by flavodoxin expression. The results indicate that ROS formed in chloroplasts have a marginal effect on plant responses during salt stress, and that sensitive targets are present in roots which are not protected by flavodoxin. PMID:27441560

  5. Low doses of ultraviolet radiation and oxidative damage induce dramatic accumulation of mitochondrial DNA replication intermediates, fork regression, and replication initiation shift.

    PubMed

    Torregrosa-Muñumer, Rubén; Goffart, Steffi; Haikonen, Juha A; Pohjoismäki, Jaakko L O

    2015-11-15

    Mitochondrial DNA is prone to damage by various intrinsic as well as environmental stressors. DNA damage can in turn cause problems for replication, resulting in replication stalling and double-strand breaks, which are suspected to be the leading cause of pathological mtDNA rearrangements. In this study, we exposed cells to subtle levels of oxidative stress or UV radiation and followed their effects on mtDNA maintenance. Although the damage did not influence mtDNA copy number, we detected a massive accumulation of RNA:DNA hybrid-containing replication intermediates, followed by an increase in cruciform DNA molecules, as well as in bidirectional replication initiation outside of the main replication origin, OH. Our results suggest that mitochondria maintain two different types of replication as an adaptation to different cellular environments; the RNA:DNA hybrid-involving replication mode maintains mtDNA integrity in tissues with low oxidative stress, and the potentially more error tolerant conventional strand-coupled replication operates when stress is high.

  6. Accumulation of DNA damage and reduced levels of nicotine adenine dinucleotide in the brains of Atm-deficient mice.

    PubMed

    Stern, Nora; Hochman, Ayala; Zemach, Naty; Weizman, Nir; Hammel, Ilan; Shiloh, Yosef; Rotman, Galit; Barzilai, Ari

    2002-01-01

    Ataxia-telangiectasia (A-T) is a human genetic disorder caused by mutational inactivation of the ATM gene. A-T patients display a pleiotropic phenotype, in which a major neurological feature is progressive ataxia due to degeneration of cerebellar Purkinje and granule neurons. Disruption of the mouse Atm locus creates a murine model of A-T that exhibits most of the clinical and cellular features of the human disease, but the neurological phenotype is barely expressed. We present evidence for the accumulation of DNA strand breaks in the brains of Atm(-/-), supporting the notion that ATM plays a major role in maintaining genomic stability. We also show a perturbation of the steady state levels of pyridine nucleotides. There is a significant decrease in both the reduced and the oxidized forms of NAD and in the total levels of NADP(T) and NADP(+) in the brains of Atm(-/-) mice. The changes in NAD(T), NADH, NAD(+), NADP(T), and NADP(+) were progressive and observed primarily in the cerebellum of 4-month-old Atm(-/-) mice. Higher rates of mitochondrial respiration were also recorded in 4-month-old Atm(-/-) cerebella. Taken together, our findings support the hypothesis that absence of functional ATM results in continuous stress, which may be an important cause of the degeneration of cerebellar neurons in A-T. PMID:11679583

  7. A Review of Spectral Methods for Variable Amplitude Fatigue Prediction and New Results

    NASA Technical Reports Server (NTRS)

    Larsen, Curtis E.; Irvine, Tom

    2013-01-01

    A comprehensive review of the available methods for estimating fatigue damage from variable amplitude loading is presented. The dependence of fatigue damage accumulation on power spectral density (psd) is investigated for random processes relevant to real structures such as in offshore or aerospace applications. Beginning with the Rayleigh (or narrow band) approximation, attempts at improved approximations or corrections to the Rayleigh approximation are examined by comparison to rainflow analysis of time histories simulated from psd functions representative of simple theoretical and real world applications. Spectral methods investigated include corrections by Wirsching and Light, Ortiz and Chen, the Dirlik formula, and the Single-Moment method, among other more recent proposed methods. Good agreement is obtained between the spectral methods and the time-domain rainflow identification for most cases, with some limitations. Guidelines are given for using the several spectral methods to increase confidence in the damage estimate.

  8. Contact fatigue of a silicon carbide with a heterogeneous grain structure

    SciTech Connect

    Padture, N.P.; Lawn, B.R.

    1995-06-01

    A comparative study of cyclic fatigue damage from Hertzian contacts in silicon carbide ceramics with homogeneous microstructure (fine, equiaxed grains, strong grain boundaries) and heterogeneous microstructure (coarse, contiguous elongate grains, weak interphase boundaries) is presented. Observations of the surface and subsurface damage patterns using optical microscopy reveal fundamentally different cyclic fatigue mechanisms: in the homogeneous material, by slow growth of a well-developed cone crack outside the contact area; in the heterogeneous material, by progressive mechanical degradation within a distributed damage zone below the contact area. Scanning electron micrographs of the latter material show copious fine debris in the damage zone, consistent with a degradation mechanism by frictional attrition by forward-reverse sliding at the weak interphase boundaries. Acoustic emission is recorded during both load and unload half-cycles, confirming hysteresis in the sliding process. Flexure tests indicate initially slight strength losses from the cyclic contact damage in both microstructures, followed by accelerated losses at higher numbers of cycles. The underlying basis for establishing an analytical model of damage accumulation in the heterogeneous microstructure in terms of shear-fault sliding, and for designing microstructures for optimal properties in fatigue and wear applications, is foreshadowed.

  9. Neurochemical evidence that the metabolites accumulating in 3-methylcrotonyl-CoA carboxylase deficiency induce oxidative damage in cerebral cortex of young rats.

    PubMed

    Zanatta, Ângela; Moura, Alana Pimentel; Tonin, Anelise Miotti; Knebel, Lisiane Aurélio; Grings, Mateus; Lobato, Vannessa Araújo; Ribeiro, César Augusto João; Dutra-Filho, Carlos Severo; Leipnitz, Guilhian; Wajner, Moacir

    2013-01-01

    Isolated 3-methylcrotonyl-CoA carboxylase deficiency (3MCCD) is an autosomal recessive disorder of leucine metabolism biochemically characterized by accumulation of 3-methylcrotonylglycine (3MCG), 3-methylcrotonic acid (3MCA) and 3-hydroxyisovaleric acid. A considerable number of affected individuals present neurological symptoms with or without precedent crises of metabolic decompensation and brain abnormalities whose pathogenesis is poorly known. We investigated the in vitro effects of 3MCG and 3MCA on important parameters of oxidative stress in cerebral cortex of young rats. 3MCG and 3MCA significantly increased TBA-RS and carbonyl formation, indicating that these compounds provoke lipid and protein oxidation, respectively. In contrast, nitric oxide production was not affected by 3MCG and 3MCA. Furthermore, 3MCG- and 3MCA-induced elevation of TBA-RS values was fully prevented by melatonin, trolox and reduced glutathione, but not by the nitric oxide inhibitor N(ω)-nitro-L-arginine methyl ester or the combination of catalase plus superoxide dismutase, indicating that reactive oxygen species were involved in the oxidative damage caused by these compounds. We also found that the activity of the antioxidant enzymes glutathione peroxidase, catalase, superoxide dismutase and glutathione reductase were not altered in vitro by 3MCG and 3MCA. It is therefore presumed that alterations of the cellular redox homeostasis caused by the major metabolites accumulating in 3MCCD may potentially be involved in the pathophysiology of the neurological dysfunction and structural brain alterations found in patients affected by this disorder.

  10. Beneficial effect of diosgenin as a stimulator of NGF on the brain with neuronal damage induced by Aβ-42 accumulation and neurotoxicant injection.

    PubMed

    Koh, Eun-Kyoung; Yun, Woo-Bin; Kim, Ji-Eun; Song, Sung-Hwa; Sung, Ji-Eun; Lee, Hyun-Ah; Seo, Eun-Ji; Jee, Seung-Wan; Bae, Chang-Joon; Hwang, Dae-Youn

    2016-06-01

    To investigate the beneficial effects of diosgenin (DG) on the multiple types of brain damage induced by Aβ-42 peptides and neurotoxicants, alterations in the specific aspects of brain functions were measured in trimethyltin (TMT)-injected transgenic 2576 (TG) mice that had been pretreated with DG for 21 days. Multiple types of damage were successfully induced by Aβ-42 accumulation and TMT injection into the brains of TG mice. However, DG treatment significantly reduced the number of Aβ-stained plaques and dead cells in the granule cells layer of the dentate gyrus. Significant suppression of acetylcholinesterase (AChE) activity and Bax/Bcl-2 expression was also observed in the DG treated TG mice (TG+DG group) when compared with those of the vehicle (VC) treated TG mice (TG+VC group). Additionally, the concentration of nerve growth factor (NGF) was dramatically enhanced in TG+DG group, although it was lower in the TG+VC group than the non-transgenic (nTG) group. Furthermore, the decreased phosphorylation of downstream members in the TrkA high affinity receptor signaling pathway in the TG+VC group was significantly recovered in the TG+DG group. A similar pattern was observed in p75(NTR) expression and JNK phosphorylation in the NGF low affinity receptor signaling pathway. Moreover, superoxide dismutase (SOD) activity was enhanced in the TG+DG group, while the level of malondialdehyde (MDA), a marker of lipid peroxidation, was lower in the TG+DG group than the TG+VC group. These results suggest that DG could exert a wide range of beneficial activities for multiple types of brain damage through stimulation of NGF biosynthesis. PMID:27382379

  11. Beneficial effect of diosgenin as a stimulator of NGF on the brain with neuronal damage induced by Aβ-42 accumulation and neurotoxicant injection

    PubMed Central

    Koh, Eun-Kyoung; Yun, Woo-Bin; Kim, Ji-Eun; Song, Sung-Hwa; Sung, Ji-Eun; Lee, Hyun-Ah; Seo, Eun-Ji; Jee, Seung-Wan

    2016-01-01

    To investigate the beneficial effects of diosgenin (DG) on the multiple types of brain damage induced by Aβ-42 peptides and neurotoxicants, alterations in the specific aspects of brain functions were measured in trimethyltin (TMT)-injected transgenic 2576 (TG) mice that had been pretreated with DG for 21 days. Multiple types of damage were successfully induced by Aβ-42 accumulation and TMT injection into the brains of TG mice. However, DG treatment significantly reduced the number of Aβ-stained plaques and dead cells in the granule cells layer of the dentate gyrus. Significant suppression of acetylcholinesterase (AChE) activity and Bax/Bcl-2 expression was also observed in the DG treated TG mice (TG+DG group) when compared with those of the vehicle (VC) treated TG mice (TG+VC group). Additionally, the concentration of nerve growth factor (NGF) was dramatically enhanced in TG+DG group, although it was lower in the TG+VC group than the non-transgenic (nTG) group. Furthermore, the decreased phosphorylation of downstream members in the TrkA high affinity receptor signaling pathway in the TG+VC group was significantly recovered in the TG+DG group. A similar pattern was observed in p75NTR expression and JNK phosphorylation in the NGF low affinity receptor signaling pathway. Moreover, superoxide dismutase (SOD) activity was enhanced in the TG+DG group, while the level of malondialdehyde (MDA), a marker of lipid peroxidation, was lower in the TG+DG group than the TG+VC group. These results suggest that DG could exert a wide range of beneficial activities for multiple types of brain damage through stimulation of NGF biosynthesis. PMID:27382379

  12. Effect of Low Temperature on Fatigue Crack Formation and Microstructure-Scale Growth from Corrosion Damage in Al-Zn-Mg-Cu

    NASA Astrophysics Data System (ADS)

    Burns, James T.; Gangloff, Richard P.

    2013-05-01

    The strong effect of cold temperature on the fatigue resistance of 7075-T651 is established. As temperature decreases from 296 K to 183 K (23 °C to -90 °C), the formation life for cracking about pit and EXCO corrosion perimeters increases, microstructure scale crack growth rates decrease in the range from 20 to 500 μm beyond the corrosion topography, and long crack growth rates similarly decline. Fatigue crack surface features correlate with reduced hydrogen embrittlement with decreasing temperature fed by localized H produced during precorrosion for pit and EXCO-proximate cracks, as well as by crack tip H produced by water vapor reaction during stressing for all crack sizes. The importance of the former H source increases with decreasing temperature for cracks sized below 200 μm. Decreasing temperature to 223 K (-50 °C) eliminates the contribution of environmental H through interaction of reduced water vapor pressure in equilibrium with ice and reduced H diffusion. The Knudsen flow model and exposure parameter, P_{{{{H}}2 {{O}}}}/f , enables improved modeling of temperature dependent crack propagation, but does not fully describe low temperature fatigue behavior due to possible rate limitation by H diffusion. Further decreases in MSC da/dN to 183 K (-90 °C) are related to reduced mobility of the corrosion-precharged H which may associate with vacancies from dissolution. Crack formation, and growth rates correlate with either elastic stress intensity range or cyclic crack tip opening displacement, and are available to predict corrosion effects on airframe fatigue for the important low temperature regime.

  13. Creep fatigue life prediction for engine hot section materials (isotropic). Annual report

    SciTech Connect

    Moreno, V.; Nissley, D.; Lin, L.J.

    1985-03-01

    The first two years of a two-phase program aimed at improving the high temperature crack initiation life prediction technology for gas turbine hot section components are discussed. In Phase 1 (baseline) effort, low cycle fatigue (LCF) models, using a data base generated for a cast nickel base gas turbine hot section alloy (B1900+Hf), were evaluated for their ability to predict the crack initiation life for relevant creep-fatigue loading conditions and to define data required for determination of model constants. The variables included strain range and rate, mean strain, strain hold times and temperature. None of the models predicted all of the life trends within reasonable data requirements. A Cycle Damage Accumulation (CDA) was therefore developed which follows an exhaustion of material ductility approach. Material ductility is estimated based on observed similarities of deformation structure between fatigue, tensile and creep tests. The cycle damage function is based on total strain range, maximum stress and stress amplitude and includes both time independent and time dependent components. The CDA model accurately predicts all of the trends in creep-fatigue life with loading conditions. In addition, all of the CDA model constants are determinable from rapid cycle, fully reversed fatigue tests and monotonic tensile and/or creep data.

  14. Inelastic Strain Analysis of Solder Joint in NASA Fatigue Specimen

    NASA Technical Reports Server (NTRS)

    Dasgupta, Abhijit; Oyan, Chen

    1991-01-01

    The solder fatigue specimen designed by NASA-GSFC/UNISYS is analyzed in order to obtain the inelastic strain history during two different representative temperature cycles specified by UNISYS. In previous reports (dated July 25, 1990, and November 15, 1990), results were presented of the elastic-plastic and creep analysis for delta T = 31 C cycle, respectively. Subsequent results obtained during the current phase, from viscoplastic finite element analysis of the solder fatigue specimen for delta T = 113 C cycle are summarized. Some common information is repeated for self-completeness. Large-deformation continuum formulations in conjunction with a standard linear solid model is utilized for modeling the solder constitutive creep-plasticity behavior. Relevant material properties are obtained from the literature. Strain amplitudes, mean strains, and residual strains (as well as stresses) accumulated due to a representative complete temperature cycle are obtained as a result of this analysis. The partitioning between elastic strains, time-independent inelastic (plastic) strains, and time-dependent inelastic (creep) strains is also explicitly obtained for two representative cycles. Detailed plots are presented for two representative temperature cycles. This information forms an important input for fatigue damage models, when predicting the fatigue life of solder joints under thermal cycling

  15. Characterization of a soft elastomeric capacitive strain sensor for fatigue crack monitoring

    NASA Astrophysics Data System (ADS)

    Kong, Xiangxiong; Li, Jian; Laflamme, Simon; Bennett, Caroline; Matamoros, Adolfo

    2015-04-01

    Fatigue cracks have been one of the major factors for the deterioration of steel bridges. In order to maintain structural integrity, monitoring fatigue crack activities such as crack initiation and propagation is critical to prevent catastrophic failure of steel bridges due to the accumulation of fatigue damage. Measuring the strain change under cracking is an effective way of monitoring fatigue cracks. However, traditional strain sensors such as metal foil gauges are not able to capture crack development due to their small size, limited measurement range, and high failure rate under harsh environmental conditions. Recently, a newly developed soft elastomeric capacitive sensor has great promise to overcome these limitations. In this paper, crack detection capability of the capacitive sensor is demonstrated through Finite Element (FE) analysis. A nonlinear FE model of a standard ASTM compact tension specimen is created which is calibrated to experimental data to simulate its response under fatigue loading, with the goal to 1) depict the strain distribution of the specimen under the large area covered by the capacitive sensor due to cracking; 2) characterize the relationship between capacitance change and crack width; 3) quantify the minimum required resolution of data acquisition system for detecting the fatigue cracks. The minimum resolution serves as a basis for the development of a dedicated wireless data acquisition system for the capacitive strain sensor.

  16. [Progress of researches on prevention and treatment of sports fatigue with moxibustion therapy].

    PubMed

    Xu, Hui-Qian; Zhang, Hong-Ru; Gu, Yi-Huang

    2014-04-01

    Sports fatigue belongs to the category of functional deficiency-syndrome according to the theory of traditional Chinese medicine. The moxibustion therapy has a long history and possesses a definite therapeutic effect in the prevention and treatment of sports fatigue. In the present paper, the authors reviewed development of researches on the effects of moxibustion intervention in the prevention and treatment of sports fatigue in recent 5 years. Results of researches showed that moxibustion intervention can 1) eliminate free radicals and reduce oxidative damage; 2) increase energy (glycogen) supply to delay the production of fatigue; 3) raise serum testosterone level (relieve exercise-induced neuroendocrine disorder) and reduce post-sports fatigue; 4) raise the anaerobic exercise ability, reduce the accumulation of metabolic products in the body and strengthen the endurance capacity of the skeletal muscle; and 5) improve ischemic cardiac function, and suppress cardiomyocyte apopotosis, etc. However, we should further strengthen our investigations on the moxibustion therapy in the ancient classical literature and sum up academic thoughts of different academic schools in the successive dynasties, put emphasis on the large sample randomized controlled clinical trails, establish united treatment standards, etc., and provide much evidence for effectively treating sports fatigue in the future.

  17. Probabilistic fatigue methodology for six nines reliability

    NASA Technical Reports Server (NTRS)

    Everett, R. A., Jr.; Bartlett, F. D., Jr.; Elber, Wolf

    1990-01-01

    Fleet readiness and flight safety strongly depend on the degree of reliability that can be designed into rotorcraft flight critical components. The current U.S. Army fatigue life specification for new rotorcraft is the so-called six nines reliability, or a probability of failure of one in a million. The progress of a round robin which was established by the American Helicopter Society (AHS) Subcommittee for Fatigue and Damage Tolerance is reviewed to investigate reliability-based fatigue methodology. The participants in this cooperative effort are in the U.S. Army Aviation Systems Command (AVSCOM) and the rotorcraft industry. One phase of the joint activity examined fatigue reliability under uniquely defined conditions for which only one answer was correct. The other phases were set up to learn how the different industry methods in defining fatigue strength affected the mean fatigue life and reliability calculations. Hence, constant amplitude and spectrum fatigue test data were provided so that each participant could perform their standard fatigue life analysis. As a result of this round robin, the probabilistic logic which includes both fatigue strength and spectrum loading variability in developing a consistant reliability analysis was established. In this first study, the reliability analysis was limited to the linear cumulative damage approach. However, it is expected that superior fatigue life prediction methods will ultimately be developed through this open AHS forum. To that end, these preliminary results were useful in identifying some topics for additional study.

  18. The Snow Must Go On: Ground Ice Encasement, Snow Compaction and Absence of Snow Differently Cause Soil Hypoxia, CO2 Accumulation and Tree Seedling Damage in Boreal Forest.

    PubMed

    Martz, Françoise; Vuosku, Jaana; Ovaskainen, Anu; Stark, Sari; Rautio, Pasi

    2016-01-01

    At high latitudes, the climate has warmed at twice the rate of the global average with most changes observed in autumn, winter and spring. Increasing winter temperatures and wide temperature fluctuations are leading to more frequent rain-on-snow events and freeze-thaw cycles causing snow compaction and formation of ice layers in the snowpack, thus creating ice encasement (IE). By decreasing the snowpack insulation capacity and restricting soil-atmosphere gas exchange, modification of the snow properties may lead to colder soil but also to hypoxia and accumulation of trace gases in the subnivean environment. To test the effects of these overwintering conditions changes on plant winter survival and growth, we established a snow manipulation experiment in a coniferous forest in Northern Finland with Norway spruce and Scots pine seedlings. In addition to ambient conditions and prevention of IE, we applied three snow manipulation levels: IE created by artificial rain-on-snow events, snow compaction and complete snow removal. Snow removal led to deeper soil frost during winter, but no clear effect of IE or snow compaction done in early winter was observed on soil temperature. Hypoxia and accumulation of CO2 were highest in the IE plots but, more importantly, the duration of CO2 concentration above 5% was 17 days in IE plots compared to 0 days in ambient plots. IE was the most damaging winter condition for both species, decreasing the proportion of healthy seedlings by 47% for spruce and 76% for pine compared to ambient conditions. Seedlings in all three treatments tended to grow less than seedlings in ambient conditions but only IE had a significant effect on spruce growth. Our results demonstrate a negative impact of winter climate change on boreal forest regeneration and productivity. Changing snow conditions may thus partially mitigate the positive effect of increasing growing season temperatures on boreal forest productivity.

  19. The Snow Must Go On: Ground Ice Encasement, Snow Compaction and Absence of Snow Differently Cause Soil Hypoxia, CO2 Accumulation and Tree Seedling Damage in Boreal Forest.

    PubMed

    Martz, Françoise; Vuosku, Jaana; Ovaskainen, Anu; Stark, Sari; Rautio, Pasi

    2016-01-01

    At high latitudes, the climate has warmed at twice the rate of the global average with most changes observed in autumn, winter and spring. Increasing winter temperatures and wide temperature fluctuations are leading to more frequent rain-on-snow events and freeze-thaw cycles causing snow compaction and formation of ice layers in the snowpack, thus creating ice encasement (IE). By decreasing the snowpack insulation capacity and restricting soil-atmosphere gas exchange, modification of the snow properties may lead to colder soil but also to hypoxia and accumulation of trace gases in the subnivean environment. To test the effects of these overwintering conditions changes on plant winter survival and growth, we established a snow manipulation experiment in a coniferous forest in Northern Finland with Norway spruce and Scots pine seedlings. In addition to ambient conditions and prevention of IE, we applied three snow manipulation levels: IE created by artificial rain-on-snow events, snow compaction and complete snow removal. Snow removal led to deeper soil frost during winter, but no clear effect of IE or snow compaction done in early winter was observed on soil temperature. Hypoxia and accumulation of CO2 were highest in the IE plots but, more importantly, the duration of CO2 concentration above 5% was 17 days in IE plots compared to 0 days in ambient plots. IE was the most damaging winter condition for both species, decreasing the proportion of healthy seedlings by 47% for spruce and 76% for pine compared to ambient conditions. Seedlings in all three treatments tended to grow less than seedlings in ambient conditions but only IE had a significant effect on spruce growth. Our results demonstrate a negative impact of winter climate change on boreal forest regeneration and productivity. Changing snow conditions may thus partially mitigate the positive effect of increasing growing season temperatures on boreal forest productivity. PMID:27254100

  20. The Snow Must Go On: Ground Ice Encasement, Snow Compaction and Absence of Snow Differently Cause Soil Hypoxia, CO2 Accumulation and Tree Seedling Damage in Boreal Forest

    PubMed Central

    Vuosku, Jaana; Ovaskainen, Anu; Stark, Sari; Rautio, Pasi

    2016-01-01

    At high latitudes, the climate has warmed at twice the rate of the global average with most changes observed in autumn, winter and spring. Increasing winter temperatures and wide temperature fluctuations are leading to more frequent rain-on-snow events and freeze-thaw cycles causing snow compaction and formation of ice layers in the snowpack, thus creating ice encasement (IE). By decreasing the snowpack insulation capacity and restricting soil-atmosphere gas exchange, modification of the snow properties may lead to colder soil but also to hypoxia and accumulation of trace gases in the subnivean environment. To test the effects of these overwintering conditions changes on plant winter survival and growth, we established a snow manipulation experiment in a coniferous forest in Northern Finland with Norway spruce and Scots pine seedlings. In addition to ambient conditions and prevention of IE, we applied three snow manipulation levels: IE created by artificial rain-on-snow events, snow compaction and complete snow removal. Snow removal led to deeper soil frost during winter, but no clear effect of IE or snow compaction done in early winter was observed on soil temperature. Hypoxia and accumulation of CO2 were highest in the IE plots but, more importantly, the duration of CO2 concentration above 5% was 17 days in IE plots compared to 0 days in ambient plots. IE was the most damaging winter condition for both species, decreasing the proportion of healthy seedlings by 47% for spruce and 76% for pine compared to ambient conditions. Seedlings in all three treatments tended to grow less than seedlings in ambient conditions but only IE had a significant effect on spruce growth. Our results demonstrate a negative impact of winter climate change on boreal forest regeneration and productivity. Changing snow conditions may thus partially mitigate the positive effect of increasing growing season temperatures on boreal forest productivity. PMID:27254100

  1. Fatigue management in the workplace

    PubMed Central

    Sadeghniiat-Haghighi, Khosro; Yazdi, Zohreh

    2015-01-01

    Workers’ fatigue is a significant problem in modern industry, largely because of high demand jobs, long duty periods, disruption of circadian rhythms, and accumulative sleep debt that are common in many industries. Fatigue is the end result of integration of multiple factors such as time awake, time of day, and workload. Then, the full understanding of circadian biologic clock, dynamics of transient and cumulative sleep loss, and recovery is required for effective management of workplace fatigue. It can be more investigated in a new field of sleep medicine called occupational sleep medicine. Occupational sleep medicine is concerned with maintaining best productivity and safety in the industrial settings. The fatigue risk management system (FRMS) is a comprehensive approach that is based on applying scientific evidence of sleep knowledge to manage workers fatigue. It is developing rapidly in the highly safety demand jobs; especially truck drivers, pilots, and power plant workers. The objective of this review is to explain about fatigue in the workplace with emphasis on its association work performance and errors/accidents. Also, we discussed about different methods of fatigue measurement and management. PMID:26257477

  2. Non Destructive Characterization of Cortical Bone Micro-Damage by Nonlinear Resonant Ultrasound Spectroscopy

    PubMed Central

    Haupert, Sylvain; Guérard, Sandra; Peyrin, Françoise; Mitton, David; Laugier, Pascal

    2014-01-01

    The objective of the study was to evaluate the ability of a nonlinear ultrasound technique, the so-called nonlinear resonant ultrasound spectroscopy (NRUS) technique, for detecting early microdamage accumulation in cortical bone induced by four-point bending fatigue. Small parallelepiped beam-shaped human cortical bone specimens were subjected to cyclic four-point bending fatigue in several steps. The specimens were prepared to control damage localization during four-point bending fatigue cycling and to unambiguously identify resonant modes for NRUS measurements. NRUS measurements were achieved to follow the evolution of the nonlinear hysteretic elastic behavior during fatigue-induced damage. After each fatigue step, a small number of specimens was removed from the protocol and set apart to quantitatively assess the microcrack number density and length using synchrotron radiation micro-computed tomography (SR-µCT). The results showed a significant effect of damage steps on the nonlinear hysteretic elastic behavior. No significant change in the overall length of microcracks was observed in damaged regions compared to the load-free control regions. Only an increased number of shortest microcracks, those in the lowest quartile, was noticed. This was suggestive of newly formed microcracks during the early phases of damage accumulation. The variation of nonlinear hysteretic elastic behavior was significantly correlated to the variation of the density of short microcracks. Our results suggest that the nonlinear hysteretic elastic behavior is sensitive to early bone microdamage. Therefore NRUS technique can be used to monitor fatigue microdamage progression in in vitro experiments. PMID:24392089

  3. Vibration fatigue analysis and multi-axial effect in testing of aerospace structures

    NASA Astrophysics Data System (ADS)

    Aykan, Murat; Çelik, Mehmet

    2009-04-01

    The work reported in this paper compared the fatigue damage accumulated under uni-axial loading (a procedure promoted by the vibration testing standards) to that induced by multi-axial loading. The comparison was performed for a helicopter structural element (the flare dispenser bracket of the self-defensive system's Chaff), which is exposed to the particular combination of wide-band random with sinusoidal vibrations, which is characteristic to the helicopter dynamic environment. The evaluation of the fatigue damage induced by these loads requires the calculation or measurement of the structure's dynamic response in terms of stresses or strains, and the application of the appropriate methodology to this response. In this work, dynamic response was calculated in the frequency domain based on the relations between the power spectral density matrixes of the excitations to that of the responses for a linear system. The transfer matrix that relates the excitation to the responses was evaluated numerically. The power spectral densities of the responses evaluated at different locations on the structure were used in the determination of the responses' statistics (the counting of the loading cycles), which, combined with an appropriate physics of failure model (fatigue model), enabled the evaluation of the accumulated fatigue damage. The uni-axial-induced fatigue was evaluated from vibration tests of the kind promoted by military standards (it is assumed that axis-by-axis loading is cumulatively equal to multi-axial loading), and compared to that evaluated by analysis for the multi-axial loading. Also a numerical comparison of the effects of the two kinds of loading was performed. The results showed that the error of uni-axial testing varied for a wide range of parameters. The work led to the conclusion that simultaneous multi-axis vibration testing can improve significantly the laboratory's vibration simulation realism.

  4. Chronic Fatigue Syndrome

    MedlinePlus

    Chronic fatigue syndrome (CFS) is a disorder that causes extreme fatigue. This fatigue is not the kind of tired feeling that ... activities. The main symptom of CFS is severe fatigue that lasts for 6 months or more. You ...

  5. Numerical Modeling of the Surface Fatigue Crack Propagation Including the Closure Effect

    NASA Astrophysics Data System (ADS)

    Guchinsky, Ruslan; Petinov, Sergei

    2016-01-01

    Presently modeling of surface fatigue crack growth for residual life assessment of structural elements is almost entirely based on application of the Linear Elastic Fracture Mechanics (LEFM). Generally, it is assumed that the crack front does not essentially change its shape, although it is not always confirmed by experiment. Furthermore, LEFM approach cannot be applied when the stress singularity vanishes due to material plasticity, one of the leading factors associated with the material degradation and fracture. Also, evaluation of stress intensity factors meets difficulties associated with changes in the stress state along the crack front circumference. An approach proposed for simulation the evolution of surface cracks based on application of the Strain-life criterion for fatigue failure and of the finite element modeling of damage accumulation. It takes into account the crack closure effect, the nonlinear behavior of damage accumulation and material compliance increasing due to the damage advance. The damage accumulation technique was applied to model the semi-elliptical crack growth from the initial defect in the steel compact specimen. The results of simulation are in good agreement with the published experimental data.

  6. Impaired response to oxidative stress in senescent cells may lead to accumulation of DNA damage in mesothelial cells from aged donors

    SciTech Connect

    Ksiazek, Krzysztof Piatek, Katarzyna; Witowski, Janusz

    2008-08-22

    The accumulation of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) exemplifies oxidative DNA injury, which is strongly implicated in ageing. We show that human peritoneal mesothelial cells (HPMCs) from donors >75 years have lower proliferative capacity but increased 8-OH-dG content compared with cells from individuals <25 years. We detected a positive relationship between the donor's age and the 8-OH-dG level in early-passage HPMCs, and an inverse relationship between those 8-OH-dG levels and subsequent replicative lifespan of HPMCs (n = 30). In early-passage cells from donors >75 years, the repair of oxidant-induced 8-OH-dG was delayed compared to cells from donors <25 years. This was coupled with prolonged removal of reactive oxygen species and faster decline in superoxide dismutase activity. Similar effects were observed in HPMCs rendered senescent in vitro. These results indicate that increased 8-OH-dG levels in HPMCs from aged individuals may reflect the in vivo presence of senescent cells with increased vulnerability to oxidative stress-induced DNA damage.

  7. Creep-fatigue of High Temperature Materials for VHTR: Effect of Cyclic Loading and Environment

    SciTech Connect

    Celine Cabet; L. Carroll; R. Wright; R. Madland

    2011-05-01

    Alloy 617 is the one of the leading candidate materials for Intermediate Heat eXchangers (IHX) of a Very High Temperature Reactor (VHTR). System start-ups and shut-downs as well as power transients will produce low cycle fatigue (LCF) loadings of components. Furthermore, the anticipated IHX operating temperature, up to 950°C, is in the range of creep so that creep-fatigue interaction, which can significantly increase the fatigue crack growth, may be one of the primary IHX damage modes. To address the needs for Alloy 617 codification and licensing, a significant creep-fatigue testing program is underway at Idaho National Laboratory. Strain controlled LCF tests including hold times up to 1800s at maximum tensile strain were conducted at total strain range of 0.3% and 0.6% in air at 950°C. Creep-fatigue testing was also performed in a simulated VHTR impure helium coolant for selected experimental conditions. The creep-fatigue tests resulted in failure times up to 1000 hrs. Fatigue resistance was significantly decreased when a hold time was added at peak stress and when the total strain was increased. The fracture mode also changed from transgranular to intergranular with introduction of a tensile hold. Changes in the microstructure were methodically characterized. A combined effect of temperature, cyclic and static loading and environment was evidenced in the targeted operating conditions of the IHX. This paper This paper reviews the data previously published by Carroll and co-workers in references 10 and 11 focusing on the role of inelastic strain accumulation and of oxidation in the initiation and propagation of surface fatigue cracks.

  8. Peridynamic model for fatigue cracking.

    SciTech Connect

    Silling, Stewart Andrew; Abe Askari

    2014-10-01

    The peridynamic theory is an extension of traditional solid mechanics in which the field equations can be applied on discontinuities, such as growing cracks. This paper proposes a bond damage model within peridynamics to treat the nucleation and growth of cracks due to cyclic loading. Bond damage occurs according to the evolution of a variable called the "remaining life" of each bond that changes over time according to the cyclic strain in the bond. It is shown that the model reproduces the main features of S-N data for typical materials and also reproduces the Paris law for fatigue crack growth. Extensions of the model account for the effects of loading spectrum, fatigue limit, and variable load ratio. A three-dimensional example illustrates the nucleation and growth of a helical fatigue crack in the torsion of an aluminum alloy rod.

  9. Fatigue Fractures

    PubMed Central

    Morris, James M.

    1968-01-01

    Fatigue (or stress) fracture of bone in military recruits has been recognized for many years. Most often it is a metatarsal bone that is involved but the tarsal bones, calcaneus, tibia, fibula, femur, and pelvis are occasionally affected. Reports of such fractures in the ribs, ulna and vertebral bodies may be found in the literature. In recent years, there has been increasing awareness of the occurrence of fatigue fractures in the civilian population. Weekend sportsmen, athletes in an early phase of training, and persons engaged in unaccustomed, repetitive, vigorous activity are potential victims of such a fracture. The signs and symptoms, roentgenographic findings, treatment and etiology of fatigue fractures are dealt with in this presentation. ImagesFigure 1.Figure 2.Figure 3.Figure 4.Figure 5.Figure 6. PMID:5652745

  10. Thermal Fatigue and Fracture Behavior of Ceramic Thermal Barrier Coatings

    NASA Technical Reports Server (NTRS)

    Zhu, Dong-Ming; Choi, Sung R.; Miller, Robert A.

    2001-01-01

    Thermal fatigue and fracture behavior of plasma-sprayed ceramic thermal barrier coatings has been investigated under high heat flux and thermal cyclic conditions. The coating crack propagation is studied under laser heat flux cyclic thermal loading, and is correlated with dynamic fatigue and strength test results. The coating stress response and inelasticity, fatigue and creep interactions, and interface damage mechanisms during dynamic thermal fatigue processes are emphasized.

  11. Ammonium accumulation is a primary effect of 2-methylcitrate exposure in an in vitro model for brain damage in methylmalonic aciduria.

    PubMed

    Cudré-Cung, Hong-Phuc; Zavadakova, Petra; do Vale-Pereira, Sónia; Remacle, Noémie; Henry, Hugues; Ivanisevic, Julijana; Tavel, Denise; Braissant, Olivier; Ballhausen, Diana

    2016-09-01

    Using 3D organotypic rat brain cell cultures in aggregates we recently identified 2-methylcitrate (2-MCA) as the main toxic metabolite for developing brain cells in methylmalonic aciduria. Exposure to 2-MCA triggered morphological changes and apoptosis of brain cells. This was accompanied by increased ammonium and decreased glutamine levels. However, the sequence and causal relationship between these phenomena remained unclear. To understand the sequence and time course of pathogenic events, we exposed 3D rat brain cell aggregates to different concentrations of 2-MCA (0.1, 0.33 and 1.0mM) from day in vitro (DIV) 11 to 14. Aggregates were harvested at different time points from DIV 12 to 19. We compared the effects of a single dose of 1mM 2-MCA administered on DIV 11 to the effects of repeated doses of 1mM 2-MCA. Pan-caspase inhibitors Z-VAD FMK or Q-VD-OPh were used to block apoptosis. Ammonium accumulation in the culture medium started within few hours after the first 2-MCA exposure. Morphological changes of the developing brain cells were already visible after 17h. The highest rate of cleaved caspase-3 was observed after 72h. A dose-response relationship was observed for all effects. Surprisingly, a single dose of 1mM 2-MCA was sufficient to induce all of the biochemical and morphological changes in this model. 2-MCA-induced ammonium accumulation and morphological changes were not prevented by concomitant treatment of the cultures with pan-caspase inhibitors Z-VAD FMK or Q-VD-OPh: ammonium increased rapidly after a single 1mM 2-MCA administration even after apoptosis blockade. We conclude that following exposure to 2-MCA, ammonium production in brain cell cultures is an early phenomenon, preceding cell degeneration and apoptosis, and may actually be the cause of the other changes observed. The fact that a single dose of 1mM 2-MCA is sufficient to induce deleterious effects over several days highlights the potential damaging effects of even short-lasting metabolic

  12. Bending-fatigue damage-detection on notched-tooth spiral-bevel gears using the average-log-ratio, ALR, algorithm

    NASA Astrophysics Data System (ADS)

    Hines, Jason A.; Mark, William D.

    2014-02-01

    The frequency-domain ALR (average-log-ratio) damage-detection algorithm [MSSP 24 (2010) 2807-2823] is utilized to illustrate damage detection and progression on notched-tooth spiral-bevel gears. Use of equal weighting of increases or decreases of individual rotational-harmonic amplitudes caused by damage, for early ALR detections, is substantiated. Continuously improving statistical reliability of ALR is documented by using increasing numbers of rotational-harmonic amplitude-ratios and increasing numbers of waveforms in the synchronous averaging. Sensitivity of the ALR algorithm to incipient damage is observed to be comparable to that obtained from the kurtosis-based Figure of Merit 4 (FM4). In contrast to FM4, ALR is shown to monotonically increase with increasing damage and running time. Interestingly, this diagnostic technique can be implemented with remarkably low analog-to-digital conversion rates. Computation of ALR for differing torque levels shows strong indications of weakening tooth-stiffness and increasing tooth-plastic-deformation. ALR computation utilizing tooth-rotational-location windowing also is illustrated.

  13. Lead accumulation, oxidative damage and histopathological alteration in testes and accessory glands of freshwater crab, Sinopotamon henanense, induced by acute lead exposure.

    PubMed

    Li, Na; Hou, Yu-hua; Ma, Dan-dan; Jing, Wei-xin; Dahms, Hans-Uwe; Wang, Lan

    2015-07-01

    Lead (Pb) is one of the most toxic environmental pollutants and known to exert multiple toxic effects including gonadotoxic and spermiotoxic effects. In order to understand toxic mechanisms of lead (Pb) on the testes and the accessory glands of crabs, we investigated Pb accumulation in testes and accessory glands and the survival rate of sperms of freshwater crab, Sinopotamon henanense. The tissue damaging effects of Pb was also investigated by histopathological examination and analyses of antioxidant enzymes as well as lipid peroxidation. Crabs were exposed to different Pb concentrations (0, 3.675, 7.35, 14.7, 29.4 and 58.8 mg/L) for 3, 5 and 7 days. The results showed that Pb levels in testes and accessory glands increased significantly following Pb exposure for 5 and 7 days in almost all treated groups, and survival rate of sperm decreased with increasing Pb concentrations at 5 and 7 days. Morphological changes identified histologically were discovered in testes, including a disordered arrangement of germ cells, a decreased number of sperm in the lumina of the seminiferous tubules, extensive necrosis in the germinal layer of the seminiferous tubules, etc. At the same time, histological abnormalities were discovered in accessory glands, the wall cells were separated from the basement membrane, and wall cells were missing partly. The activities of SOD, GPx and CAT in testes showed no statistically significant changes compared to the control for 3 days, and initially increased and subsequently decreased with increasing Pb concentrations at 5 and 7 days. The antioxidant enzyme activities in accessory glands initially increased and subsequently decreased with increasing Pb concentrations and Pb exposure. This was accompanied with an increase in malondialdehyde (MDA) content in a concentration-dependent manner. These results showed that acute Pb exposure led to a reduction of survival rate of sperm and harmful effects at the cellular level of crab testes and accessory

  14. Microstructural aspects of fatigue in Ni-base superalloys.

    PubMed

    Antolovich, Stephen D

    2015-03-28

    Nickel-base superalloys are primarily used as components in jet engines and land-based turbines. While compositionally complex, they are microstructurally simple, consisting of small (50-1000 nm diameter), ordered, coherent Ni(3)(Al,Ti)-type L1(2) or Ni(3)Nb-type DO(22) precipitates (called γ(') and γ(''), respectively) embedded in an FCC substitutional solid solution consisting primarily of Ni and other elements which confer desired properties depending upon the application. The grain size may vary from as small as 2 μm for powder metallurgy alloys used in discs to single crystals the actual size of the component for turbine blades. The fatigue behaviour depends upon the microstructure, deformation mode, environment and cycle time. In many cases, it can be controlled or modified through small changes in composition which may dramatically change the mechanism of damage accumulation and the fatigue life. In this paper, the fundamental microstructural, compositional, environmental and deformation mode factors which affect fatigue behaviour are critically reviewed. Connections are made across a range of studies to provide more insight. Modern approaches are pointed out in which the wealth of available microstructural, deformation and damage information is used for computerized life prediction. The paper ends with a discussion of the very important and highly practical subject of thermo-mechanical fatigue (TMF). It is shown that physics-based modelling leads to significantly improved life prediction. Suggestions are made for moving forward on the critical subject of TMF life prediction in notched components. PMID:25713453

  15. Evaluation of the strength and creep-fatigue behavior of hot isostatically pressed silicon nitride

    SciTech Connect

    Ferber, M.K.; Jenkins, M.G. )

    1992-09-01

    This paper reports that the strength of a commerically available hot isostatically pressed silicon nitride was measured as a function of temperature. To evaluate long-term mechanical reliability of this material, the tensile creep and fatigue behavior was measured at 1150[degrees], 1260[degrees], and 1370[degrees]C. The stress and temperature sensitivities of the secondary (or minimum) creep strain rate were used to estimate the stress exponent and activation energy associated with the dominant creep mechanism. The fatigue characteristics were evaluated by allowing individual creep tests to continue until specimen failure. The applicability of the four-point load geometry to the study of strength and creep behavior was also determined by conducting a limited number of flexural creep tests. The tensile fatigue data revealed two distinct failure mechanisms. At 1150[degrees]C, failure was controlled by a slow crack growth mechanism. At 1260[degrees] and 1370[degrees]C, the accumulation of creep damage in the form of grain boundary cavities and cracks dominated the fatigue behavior. In this temperature regime, the fatigue life was controlled by the secondary (or minimum) creep strain rate in accordance with the Monkman-Grant relation.

  16. Effect of Phosphate-Buffered Solution Corrosion on the Ratcheting Fatigue Behavior of a Duplex Mg-Li-Al Alloy

    NASA Astrophysics Data System (ADS)

    Yuan, Xin; Yu, Dunji; Gao, Li-Lan; Gao, Hong

    2016-05-01

    This work reports the uniaxial ratcheting and fatigue behavior of a duplex Mg-Li-Al alloy under the influence of phosphate-buffered solution corrosion. Microstructural observations reveal pitting and filament corrosion defects, which impair the load-bearing capacity of the alloy and cause stress concentration, thus leading to an accelerated accumulation of ratcheting strain and shortened fatigue life under the same nominal loading conditions. Comparing Smith model, Smith-Watson-Topper model, and Paul-Sivaprasad-Dhar model, a ratcheting fatigue life prediction model based on the Broberg damage rule and the Paul-Sivaprasad-Dhar model was proposed, and the model yielded a superior prediction for the studied magnesium alloy.

  17. Computational predictive methods for fracture and fatigue

    NASA Astrophysics Data System (ADS)

    Cordes, J.; Chang, A. T.; Nelson, N.; Kim, Y.

    1994-09-01

    The damage-tolerant design philosophy as used by aircraft industries enables aircraft components and aircraft structures to operate safely with minor damage, small cracks, and flaws. Maintenance and inspection procedures insure that damages developed during service remain below design values. When damage is found, repairs or design modifications are implemented and flight is resumed. Design and redesign guidelines, such as military specifications MIL-A-83444, have successfully reduced the incidence of damage and cracks. However, fatigue cracks continue to appear in aircraft well before the design life has expired. The F16 airplane, for instance, developed small cracks in the engine mount, wing support, bulk heads, the fuselage upper skin, the fuel shelf joints, and along the upper wings. Some cracks were found after 600 hours of the 8000 hour design service life and design modifications were required. Tests on the F16 plane showed that the design loading conditions were close to the predicted loading conditions. Improvements to analytic methods for predicting fatigue crack growth adjacent to holes, when multiple damage sites are present, and in corrosive environments would result in more cost-effective designs, fewer repairs, and fewer redesigns. The overall objective of the research described in this paper is to develop, verify, and extend the computational efficiency of analysis procedures necessary for damage tolerant design. This paper describes an elastic/plastic fracture method and an associated fatigue analysis method for damage tolerant design. Both methods are unique in that material parameters such as fracture toughness, R-curve data, and fatigue constants are not required. The methods are implemented with a general-purpose finite element package. Several proof-of-concept examples are given. With further development, the methods could be extended for analysis of multi-site damage, creep-fatigue, and corrosion fatigue problems.

  18. Computational predictive methods for fracture and fatigue

    NASA Technical Reports Server (NTRS)

    Cordes, J.; Chang, A. T.; Nelson, N.; Kim, Y.

    1994-01-01

    The damage-tolerant design philosophy as used by aircraft industries enables aircraft components and aircraft structures to operate safely with minor damage, small cracks, and flaws. Maintenance and inspection procedures insure that damages developed during service remain below design values. When damage is found, repairs or design modifications are implemented and flight is resumed. Design and redesign guidelines, such as military specifications MIL-A-83444, have successfully reduced the incidence of damage and cracks. However, fatigue cracks continue to appear in aircraft well before the design life has expired. The F16 airplane, for instance, developed small cracks in the engine mount, wing support, bulk heads, the fuselage upper skin, the fuel shelf joints, and along the upper wings. Some cracks were found after 600 hours of the 8000 hour design service life and design modifications were required. Tests on the F16 plane showed that the design loading conditions were close to the predicted loading conditions. Improvements to analytic methods for predicting fatigue crack growth adjacent to holes, when multiple damage sites are present, and in corrosive environments would result in more cost-effective designs, fewer repairs, and fewer redesigns. The overall objective of the research described in this paper is to develop, verify, and extend the computational efficiency of analysis procedures necessary for damage tolerant design. This paper describes an elastic/plastic fracture method and an associated fatigue analysis method for damage tolerant design. Both methods are unique in that material parameters such as fracture toughness, R-curve data, and fatigue constants are not required. The methods are implemented with a general-purpose finite element package. Several proof-of-concept examples are given. With further development, the methods could be extended for analysis of multi-site damage, creep-fatigue, and corrosion fatigue problems.

  19. Interactive effects of ultraviolet-B radiation and pesticide exposure on DNA photo-adduct accumulation and expression of DNA damage and repair genes in Xenopus laevis embryos.

    PubMed

    Yu, Shuangying; Tang, Song; Mayer, Gregory D; Cobb, George P; Maul, Jonathan D

    2015-02-01

    Pesticide use and ultraviolet-B (UVB) radiation have both been suggested to adversely affect amphibians; however, little is known about their interactive effects. One potential adverse interaction could involve pesticide-induced dysregulation of DNA repair pathways, resulting in greater numbers of DNA photo-adducts from UVB exposure. In the present study, we investigated the interactive effects of UVB radiation and two common pesticides (endosulfan and α-cypermethrin) on induction of DNA photo-adducts and expression of DNA damage and repair related genes in African clawed frog (Xenopus laevis) embryos. We examined 13 genes that are, collectively, involved in stress defense, cell cycle arrest, nucleotide excision repair (NER), base excision repair, mismatch repair, DNA repair regulation, and apoptosis. We exposed X. laevis embryos to 0, 25, and 50 μg/L endosulfan or 0, 2.5, and 5.0 μg/L α-cypermethrin for 96 h, with environmentally relevant exposures of UVB radiation during the last 7 h of the 96 h exposure. We measured the amount of cyclobutane pyrimidine dimers (CPDs) and mRNA abundance of the 13 genes among treatments including control, pesticide only, UVB only, and UVB and pesticide co-exposures. Each of the co-exposure scenarios resulted in elevated CPD levels compared to UVB exposure alone, suggesting an inhibitory effect of endosulfan and α-cypermethrin on CPD repair. This is attributed to results indicating that α-cypermethrin and endosulfan reduced mRNA abundance of XPA and HR23B, respectively, to levels that may affect the initial recognition of DNA lesions. In contrast, both pesticides increased transcript abundance of CSA and MUTL. In addition, mRNA abundance of HSP70 and GADD45α were increased by endosulfan and mRNA abundance of XPG was increased by α-cypermethrin. XPC, HR23B, XPG, and GADD45α exhibited elevated mRNA concentrations whereas there was a reduction in MUTL transcript concentrations in UVB-alone treatments. It appeared that even

  20. Accumulate repeat accumulate codes

    NASA Technical Reports Server (NTRS)

    Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

    2004-01-01

    In this paper we propose an innovative channel coding scheme called 'Accumulate Repeat Accumulate codes' (ARA). This class of codes can be viewed as serial turbo-like codes, or as a subclass of Low Density Parity Check (LDPC) codes, thus belief propagation can be used for iterative decoding of ARA codes on a graph. The structure of encoder for this class can be viewed as precoded Repeat Accumulate (RA) code or as precoded Irregular Repeat Accumulate (IRA) code, where simply an accumulator is chosen as a precoder. Thus ARA codes have simple, and very fast encoder structure when they representing LDPC codes. Based on density evolution for LDPC codes through some examples for ARA codes, we show that for maximum variable node degree 5 a minimum bit SNR as low as 0.08 dB from channel capacity for rate 1/2 can be achieved as the block size goes to infinity. Thus based on fixed low maximum variable node degree, its threshold outperforms not only the RA and IRA codes but also the best known LDPC codes with the dame maximum node degree. Furthermore by puncturing the accumulators any desired high rate codes close to code rate 1 can be obtained with thresholds that stay close to the channel capacity thresholds uniformly. Iterative decoding simulation results are provided. The ARA codes also have projected graph or protograph representation that allows for high speed decoder implementation.

  1. Fatigue of bovine trabecular bone.

    PubMed

    Moore, Tara L; Gibson, Lorna J

    2003-12-01

    Fatigue loading of bone, from the activities of daily living in the elderly, or from prolonged exercise in the young, can lead to increased risk of fracture. Elderly patients with osteoporosis are particularly prone to fragility fractures of the vertebrae, where load is carried primarily by trabecular bone. In this study, specimens of bovine trabecular bone were loaded in compressive fatigue at four different normalized stresses to one of six maximum strains. The resulting change in modulus and residual strain accumulation were measured over the life of the fatigue test. The number of cycles to reach a given maximum compressive strain increased with decreasing normalized stress. Modulus reduction and specimen residual strain increased with increasing maximum compressive strain, but few differences were observed between specimens loaded to the same maximum strain at different normalized stresses.

  2. Assessment of fatigue life of remanufactured impeller based on FEA

    NASA Astrophysics Data System (ADS)

    Xu, Lei; Cao, Huajun; Liu, Hailong; Zhang, Yubo

    2016-09-01

    Predicting the fatigue life of remanufactured centrifugal compressor impellers is a critical problem. In this paper, the S-N curve data were obtained by combining experimentation and theory deduction. The load spectrum was compiled by the rain-flow counting method based on the comprehensive consideration of the centrifugal force, residual stress, and aerodynamic loads in the repair region. A fatigue life simulation model was built, and fatigue life was analyzed based on the fatigue cumulative damage rule. Although incapable of providing a high-precision prediction, the simulation results were useful for the analysis of fatigue life impact factors and fatigue fracture areas. Results showed that the load amplitude greatly affected fatigue life, the impeller was protected from running at over-speed, and the predicted fatigue life was satisfied within the next service cycle safely at the rated speed.

  3. Assessment of fatigue life of remanufactured impeller based on FEA

    NASA Astrophysics Data System (ADS)

    Xu, Lei; Cao, Huajun; Liu, Hailong; Zhang, Yubo

    2016-07-01

    Predicting the fatigue life of remanufactured centrifugal compressor impellers is a critical problem. In this paper, the S-N curve data were obtained by combining experimentation and theory deduction. The load spectrum was compiled by the rain-flow counting method based on the comprehensive consideration of the centrifugal force, residual stress, and aerodynamic loads in the repair region. A fatigue life simulation model was built, and fatigue life was analyzed based on the fatigue cumulative damage rule. Although incapable of providing a high-precision prediction, the simulation results were useful for the analysis of fatigue life impact factors and fatigue fracture areas. Results showed that the load amplitude greatly affected fatigue life, the impeller was protected from running at over-speed, and the predicted fatigue life was satisfied within the next service cycle safely at the rated speed.

  4. Assessment of Solder Joint Fatigue Life Under Realistic Service Conditions

    NASA Astrophysics Data System (ADS)

    Hamasha, Sa'd.; Jaradat, Younis; Qasaimeh, Awni; Obaidat, Mazin; Borgesen, Peter

    2014-12-01

    The behavior of lead-free solder alloys under complex loading scenarios is still not well understood. Common damage accumulation rules fail to account for strong effects of variations in cycling amplitude, and random vibration test results cannot be interpreted in terms of performance under realistic service conditions. This is a result of the effects of cycling parameters on materials properties. These effects are not yet fully understood or quantitatively predictable, preventing modeling based on parameters such as strain, work, or entropy. Depending on the actual spectrum of amplitudes, Miner's rule of linear damage accumulation has been shown to overestimate life by more than an order of magnitude, and greater errors are predicted for other combinations. Consequences may be particularly critical for so-called environmental stress screening. Damage accumulation has, however, been shown to scale with the inelastic work done, even if amplitudes vary. This and the observation of effects of loading history on subsequent work per cycle provide for a modified damage accumulation rule which allows for the prediction of life. Individual joints of four different Sn-Ag-Cu-based solder alloys (SAC305, SAC105, SAC-Ni, and SACXplus) were cycled in shear at room temperature, alternating between two different amplitudes while monitoring the evolution of the effective stiffness and work per cycle. This helped elucidate general trends and behaviors that are expected to occur in vibrations of microelectronics assemblies. Deviations from Miner's rule varied systematically with the combination of amplitudes, the sequences of cycles, and the strain rates in each. The severity of deviations also varied systematically with Ag content in the solder, but major effects were observed for all the alloys. A systematic analysis was conducted to assess whether scenarios might exist in which the more fatigue-resistant high-Ag alloys would fail sooner than the lower-Ag ones.

  5. Ratcheting fatigue behavior of Zircaloy-2 at room temperature

    NASA Astrophysics Data System (ADS)

    Rajpurohit, R. S.; Sudhakar Rao, G.; Chattopadhyay, K.; Santhi Srinivas, N. C.; Singh, Vakil

    2016-08-01

    Nuclear core components of zirconium alloys experience asymmetric stress or strain cycling during service which leads to plastic strain accumulation and drastic reduction in fatigue life as well as dimensional instability of the component. Variables like loading rate, mean stress, and stress amplitude affect the influence of asymmetric loading. In the present investigation asymmetric stress controlled fatigue tests were conducted with mean stress from 80 to 150 MPa, stress amplitude from 270 to 340 MPa and stress rate from 30 to 750 MPa/s to study the process of plastic strain accumulation and its effect on fatigue life of Zircaloy-2 at room temperature. It was observed that with increase in mean stress and stress amplitude accumulation of ratcheting strain was increased and fatigue life was reduced. However, increase in stress rate led to improvement in fatigue life due to less accumulation of ratcheting strain.

  6. Low cycle fatigue properties of reduced activation ferritic/martensitic steels after high-dose neutron irradiation

    NASA Astrophysics Data System (ADS)

    Gaganidze, E.; Petersen, C.; Aktaa, J.; Povstyanko, A.; Prokhorov, V.; Diegele, E.; Lässer, R.

    2011-08-01

    This paper focuses on the low cycle fatigue (LCF) behaviour of reduced activation ferritic/martensitic steels irradiated to a displacement damage dose of up to 70 dpa at 330-337 °C in the BOR 60 reactor within the ARBOR 2 irradiation programme. The influence of neutron irradiation on the fatigue behaviour was determined for the as-received EUROFER97, pre-irradiation heat-treated EUROFER97 HT and F82H-mod steels. Strain-controlled push-pull loading was performed using miniaturized cylindrical specimens at a constant temperature of 330 °C with total strain ranges between 0.8% and 1.1%. Comparison of the LCF behaviour of irradiated and reference unirradiated specimens was performed for both the adequate total and inelastic strains. Neutron irradiation-induced hardening may have various effects on the fatigue behaviour of the steels. The reduction of inelastic strain in the irradiated state compared with the reference unirradiated state at common total strain amplitudes may increase fatigue lifetime. The increase in the stress at the adequate inelastic strain, by contrast, may accelerate fatigue damage accumulation. Depending on which of the two effects mentioned dominates, neutron irradiation may either extend or reduce the fatigue lifetime compared with the reference unirradiated state. The results obtained for EUROFER97 and EUROFER97 HT confirm these considerations. Most of the irradiated specimens show fatigue lifetimes comparable to those of the reference unirradiated state at adequate inelastic strains. Some irradiated specimens, however, show lifetime reduction or increase in comparison with the reference state at adequate inelastic strains.

  7. Aircraft fatigue and crack growth considering loads by structural component

    NASA Technical Reports Server (NTRS)

    Yost, J. D.

    1994-01-01

    The indisputable 1968 C-130 fatigue/crack growth data is reviewed to obtain additional useful information on fatigue and crack growth. The proven Load Environment Model concept derived empirically from F-105D multichannel recorder data is refined to a simpler method by going from 8 to 5 variables in the spectra without a decrease in accuracy. This approach provides the true fatigue/crack growth and load environment by structural component for both fatigue and strength design. Methods are presented for defining fatigue scatter and damage at crack initiation. These design tools and criteria may be used for both metal and composite aircraft structure.

  8. A comparative study of ship hull structures fatigue assessment methods

    NASA Astrophysics Data System (ADS)

    Petinov, Sergei V.; Polezhayeva, Helena A.; Yermolayeva, Natalya S.

    1992-07-01

    Several methods of fatigue assessment in ship hull structures are compared. The analysis is focused on fatigue problems of hull structures concerning: evaluation, the design state of fatigue damage of a structure formulation, and the adequacy of methods and data bases for the purpose of the analyses. To illustrate the discussion, examples of allowable nominal stress at a given fatigue life calculation are presented for bottom frame web slot and for a bottom longitudinal transverse bulkhead bracket connection in the case of a container ship. The low cycle (local strain) method is regarded as the most advantageous at present almost in all practical problems connected to fatigue.

  9. The role of creep in high temperature low cycle fatigue.

    NASA Technical Reports Server (NTRS)

    Manson, S. S.; Halford, G. R.; Spera, D. A.

    1971-01-01

    The significance of the role that creep can play in governing high-temperature, low-cycle fatigue resistance is investigated by conducting strain cycling tests on two high-temperature stainless steel alloys and making concurrent measurements of stress, temperature, and strain at various frequencies. The results are then analyzed in terms of damage imposed by creep and fatigue components. It is shown that creep can play an important and sometimes dominant role in low cycle fatigue at high temperatures. The results of the study include the findings that: (1) the simple life-fraction theory described is adequate for calculating creep damage when the cyclic creep rupture curve is used as a basis for analysis; (2) a method of universal slopes originally developed for room temperature use is sufficiently accurate at high temperature to be used to calculate pure fatigue damage; and (3) a linear creep-fatigue damage rule can explain the transitions observed from one failure mode to another.

  10. Fatigue life prediction under service load considering strengthening effect of loads below fatigue limit

    NASA Astrophysics Data System (ADS)

    Zhao, Lihui; Zheng, Songlin; Feng, Jinzhi

    2014-11-01

    Lightweight design requires an accurate life prediction for structures and components under service loading histories. However, predicted life with the existing methods seems too conservative in some cases, leading to a heavy structure. Because these methods are established on the basis that load cycles would only cause fatigue damage, ignore the strengthening effect of loads. Based on Palmgren-Miner Rule (PMR), this paper introduces a new method for fatigue life prediction under service loadings by taking into account the strengthening effect of loads below the fatigue limit. In this method, the service loadings are classified into three categories: damaging load, strengthening load and none-effect load, and the process for fatigue life prediction is divided into two stages: stage I and stage II, according to the best strengthening number of cycles. During stage I, fatigue damage is calculated considering both the strengthening and damaging effect of load cycles. While during stage II, only the damaging effect is considered. To validate this method, fatigue lives of automobile half shaft and torsion beam rear axle are calculated based on the new method and traditional methods, such as PMR and Modified Miner Rule (MMR), and fatigue tests of the two components are conducted under service loading histories. The tests results show that the percentage errors of the predicted life with the new method to mean life of tests for the two components are -3.78% and -1.76% separately, much lesser than that with PMR and MMR. By considering the strengthening effect of loads below the fatigue limit, the new method can significantly improve the accuracy for fatigue life prediction. Thus lightweight design can be fully realized in the design stage.

  11. A damage mechanics based approach to structural deterioration and reliability

    SciTech Connect

    Bhattcharya, B.; Ellingwood, B.

    1998-02-01

    Structural deterioration often occurs without perceptible manifestation. Continuum damage mechanics defines structural damage in terms of the material microstructure, and relates the damage variable to the macroscopic strength or stiffness of the structure. This enables one to predict the state of damage prior to the initiation of a macroscopic flaw, and allows one to estimate residual strength/service life of an existing structure. The accumulation of damage is a dissipative process that is governed by the laws of thermodynamics. Partial differential equations for damage growth in terms of the Helmholtz free energy are derived from fundamental thermodynamical conditions. Closed-form solutions to the equations are obtained under uniaxial loading for ductile deformation damage as a function of plastic strain, for creep damage as a function of time, and for fatigue damage as function of number of cycles. The proposed damage growth model is extended into the stochastic domain by considering fluctuations in the free energy, and closed-form solutions of the resulting stochastic differential equation are obtained in each of the three cases mentioned above. A reliability analysis of a ring-stiffened cylindrical steel shell subjected to corrosion, accidental pressure, and temperature is performed.

  12. Stochastic modeling of crack initiation and short-crack growth under creep and creep-fatigue conditions

    NASA Technical Reports Server (NTRS)

    Kitamura, Takayuki; Ghosn, Louis J.; Ohtani, Ryuichi

    1992-01-01

    A simplified stochastic model is proposed for crack initiation and short-crack growth under creep and creep-fatigue conditions. Material inhomogeneity provides the random nature of crack initiation and early growth. In the model, the influence of microstructure is introduced by the variability of: (1) damage accumulation along grain boundaries, (2) critical damage required for crack initiation or growth, and (3) the grain-boundary length. The probabilities of crack initiation and growth are derived by using convolution integrals. The model is calibrated and used to predict the crack density and crack-growth rate of short cracks of 304 stainless steel under creep and creep-fatigue conditions. The mean-crack initiation lives are predicted to be within an average deviation of about 10 percent from the experimental results. The predicted comulative distributions of crack-growth rate follow the experimental data closely. The applicability of the simplified stochastic model is discussed and the future research direction is outlined.

  13. Stochastic modeling of crack initiation and short-crack growth under creep and creep-fatigue conditions

    NASA Technical Reports Server (NTRS)

    Kitamura, Takayuki; Ghosn, Louis J.; Ohtani, Ryuichi

    1989-01-01

    A simplified stochastic model is proposed for crack initiation and short-crack growth under creep and creep-fatigue conditions. Material inhomogeneity provides the random nature of crack initiation and early growth. In the model, the influence of microstructure is introduced by the variability of: (1) damage accumulation along grain boundaries, (2) critical damage required for crack initiation or growth, and (3) the grain-boundary length. The probabilities of crack initiation and growth are derived by using convolution integrals. The model is calibrated and used to predict the crack density and crack-growth rate of short cracks of 304 stainless steel under creep and creep-fatigue conditions. The mean-crack initiation lives are predicted to be within an average deviation of about 10 percent from the experimental results. The predicted cumulative distributions of crack-growth rate follow the experimental data closely. The applicability of the simplified stochastic model is discussed and the future research direction is outlined.

  14. Probabilistic fatigue methodology and wind turbine reliability

    SciTech Connect

    Lange, C.H.

    1996-05-01

    Wind turbines subjected to highly irregular loadings due to wind, gravity, and gyroscopic effects are especially vulnerable to fatigue damage. The objective of this study is to develop and illustrate methods for the probabilistic analysis and design of fatigue-sensitive wind turbine components. A computer program (CYCLES) that estimates fatigue reliability of structural and mechanical components has been developed. A FORM/SORM analysis is used to compute failure probabilities and importance factors of the random variables. The limit state equation includes uncertainty in environmental loading, gross structural response, and local fatigue properties. Several techniques are shown to better study fatigue loads data. Common one-parameter models, such as the Rayleigh and exponential models are shown to produce dramatically different estimates of load distributions and fatigue damage. Improved fits may be achieved with the two-parameter Weibull model. High b values require better modeling of relatively large stress ranges; this is effectively done by matching at least two moments (Weibull) and better by matching still higher moments. For this purpose, a new, four-moment {open_quotes}generalized Weibull{close_quotes} model is introduced. Load and resistance factor design (LRFD) methodology for design against fatigue is proposed and demonstrated using data from two horizontal-axis wind turbines. To estimate fatigue damage, wind turbine blade loads have been represented by their first three statistical moments across a range of wind conditions. Based on the moments {mu}{sub 1}{hor_ellipsis}{mu}{sub 3}, new {open_quotes}quadratic Weibull{close_quotes} load distribution models are introduced. The fatigue reliability is found to be notably affected by the choice of load distribution model.

  15. Composite materials: Fatigue and fracture. Vol. 3

    NASA Technical Reports Server (NTRS)

    O'Brien, T. K. (Editor)

    1991-01-01

    The present volume discusses topics in the fields of matrix cracking and delamination, interlaminar fracture toughness, delamination analysis, strength and impact characteristics, and fatigue and fracture behavior. Attention is given to cooling rate effects in carbon-reinforced PEEK, the effect of porosity on flange-web corner strength, mode II delamination in toughened composites, the combined effect of matrix cracking and free edge delamination, and a 3D stress analysis of plain weave composites. Also discussed are the compression behavior of composites, damage-based notched-strength modeling, fatigue failure processes in aligned carbon-epoxy laminates, and the thermomechanical fatigue of a quasi-isotropic metal-matrix composite.

  16. Fatigue of continuous fiber reinforced metallic materials

    NASA Technical Reports Server (NTRS)

    Johnson, W. S.; Mirdamadi, M.; Bakuckas, J. G., Jr.

    1993-01-01

    The complex damage mechanisms that occur in fiber reinforced advanced metallic materials are discussed. As examples, results for several layups of SCS-6/Ti-15-3 composites are presented. Fatigue tests were conducted and analyzed for both notched and unnotched specimens at room and elevated temperatures. Test conditions included isothermal, non-isothermal, and simulated mission profile thermomechanical fatigue. Test results indicated that the stress in the 0 degree fibers is the controlling factor for fatigue life for a given test condition. An effective strain approach is presented for predicting crack initiation at notches. Fiber bridging models were applied to crack growth behavior.

  17. Predictive modelling of fatigue failure in concentrated lubricated contacts.

    PubMed

    Evans, H P; Snidle, R W; Sharif, K J; Bryant, M J

    2012-01-01

    Reducing frictional losses in response to the energy agenda will require use of less viscous lubricants causing hydrodynamically-lubricated bearings to operate with thinner films leading to "mixed lubrication" conditions in which a degree of direct interaction occurs between surfaces protected only by boundary tribofilms. The paper considers the consequences of thinner films and mixed lubrication for concentrated contacts such as those occurring between the teeth of power transmission gears and in rolling element bearings. Surface fatigue in gears remains a serious problem in demanding applications, and its solution will become more pressing with the tendency towards thinner oils. The particular form of failure examined here is micropitting, which is identified as a fatigue phenomenon occurring at the scale of the surface roughness asperities. It has emerged recently as a systemic difficulty in the operation of large scale wind turbines where it occurs in both power transmission gears and their support bearings. Predictive physical modelling of these contacts requires a transient mixed lubrication analysis for conditions in which the predicted lubricant film thickness is of the same order or significantly less than the height of surface roughness features. Numerical solvers have therefore been developed which are able to deal with situations in which transient solid contacts occur between surface asperity features under realistic engineering conditions. Results of the analysis, which reveal the detailed time-varying behaviour of pressure and film clearance, have been used to predict fatigue and damage accumulation at the scale of surface asperity features with the aim of improving understanding of the micropitting phenomenon. The possible consequences on fatigue of residual stress fields resulting from plastic deformation of surface asperities is also considered.

  18. Predictive modelling of fatigue failure in concentrated lubricated contacts.

    PubMed

    Evans, H P; Snidle, R W; Sharif, K J; Bryant, M J

    2012-01-01

    Reducing frictional losses in response to the energy agenda will require use of less viscous lubricants causing hydrodynamically-lubricated bearings to operate with thinner films leading to "mixed lubrication" conditions in which a degree of direct interaction occurs between surfaces protected only by boundary tribofilms. The paper considers the consequences of thinner films and mixed lubrication for concentrated contacts such as those occurring between the teeth of power transmission gears and in rolling element bearings. Surface fatigue in gears remains a serious problem in demanding applications, and its solution will become more pressing with the tendency towards thinner oils. The particular form of failure examined here is micropitting, which is identified as a fatigue phenomenon occurring at the scale of the surface roughness asperities. It has emerged recently as a systemic difficulty in the operation of large scale wind turbines where it occurs in both power transmission gears and their support bearings. Predictive physical modelling of these contacts requires a transient mixed lubrication analysis for conditions in which the predicted lubricant film thickness is of the same order or significantly less than the height of surface roughness features. Numerical solvers have therefore been developed which are able to deal with situations in which transient solid contacts occur between surface asperity features under realistic engineering conditions. Results of the analysis, which reveal the detailed time-varying behaviour of pressure and film clearance, have been used to predict fatigue and damage accumulation at the scale of surface asperity features with the aim of improving understanding of the micropitting phenomenon. The possible consequences on fatigue of residual stress fields resulting from plastic deformation of surface asperities is also considered. PMID:23285624

  19. Towards a damage tolerance philosophy for composite materials and structures

    NASA Technical Reports Server (NTRS)

    Obrien, T. Kevin

    1988-01-01

    A damage-threshold/fail-safe approach is proposed to ensure that composite structures are both sufficiently durable for economy of operation, as well as adequately fail-safe or damage tolerant for flight safety. Matrix cracks are assumed to exist throughout the off-axis plies. Delamination onset is predicted using a strain energy release rate characterization. Delamination growth is accounted for in one of three ways: either analytically, using delamination growth laws in conjunction with strain energy release rate analyses incorporating delamination resistance curves; experimentally, using measured stiffness loss; or conservatively, assuming delamination onset corresponds to catastrophic delamination growth. Fail-safety is assessed by accounting for the accumulation of delaminations through the thickness. A tension fatigue life prediction for composite laminates is presented as a case study to illustrate how this approach may be implemented. Suggestions are made for applying the damage-threshold/fail-safe approach to compression fatigue, tension/compression fatigue, and compression strength following low velocity impact.

  20. Towards a damage tolerance philosophy for composite materials and structures

    NASA Technical Reports Server (NTRS)

    O'Brien, T. Kevin

    1990-01-01

    A damage-threshold/fail-safe approach is proposed to ensure that composite structures are both sufficiently durable for economy of operation, as well as adequately fail-safe or damage tolerant for flight safety. Matrix cracks are assumed to exist throughout the off-axis plies. Delamination onset is predicted using a strain energy release rate characterization. Delamination growth is accounted for in one of three ways: either analytically, using delamination growth laws in conjunction with strain energy release rate analyses incorporating delamination resistance curves; experimentally, using measured stiffness loss; or conservatively, assuming delamination onset corresponds to catastrophic delamination growth. Fail-safety is assessed by accounting for the accumulation of delaminations through the thickness. A tension fatigue life prediction for composite laminates is presented as a case study to illustrate how this approach may be implemented. Suggestions are made for applying the damage-threshold/fail-safe approach to compression fatigue, tension/compression fatigue, and compression strength following low velocity impact.

  1. Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair.

    PubMed

    Zhu, Qianzheng; Wani, Gulzar; Sharma, Nidhi; Wani, Altaf

    2012-12-01

    Transcription factor II H (TFIIH) is composed of core TFIIH and Cdk-activating kinase (CAK) complexes. Besides transcription, TFIIH also participates in nucleotide excision repair (NER), verifying DNA lesions through its helicase components XPB and XPD. The assembly state of TFIIH is known to be affected by truncation mutations in xeroderma pigmentosum group G/Cockayne syndrome (XP-G/CS). Here, we showed that CAK component MAT1 was rapidly recruited to UV-induced DNA damage sites, co-localizing with core TFIIH component p62, and dispersed from the damage sites upon completion of DNA repair. While the core TFIIH-CAK association remained intact, MAT1 failed to accumulate at DNA damage sites in fibroblasts harboring XP-B or XP-B/CS mutations. Nevertheless, MAT1, XPD and XPC as well as XPG were able to accumulate at damage sites in XP-D fibroblasts, in which the core TFIIH-CAK association also remained intact. Interestingly, XPG recruitment was impaired in XP-B/CS fibroblasts derived from patients with mild phenotype, but persisted in XP-B/CS fibroblasts from severely affected patients resulting in a nonfunctional preincision complex. An examination of steady-state levels of RNA polymerase II (RNAPII) indicated that UV-induced RNAPII phosphorylation was dramatically reduced in XP-B/CS fibroblasts. These results demonstrated that the CAK rapidly disassociates from the core TFIIH upon assembly of nonfunctional preincision complex in XP-B and XP-B/CS cells. The persistency of nonfunctional preincision complex correlates with the severity exhibited by XP-B patients. The results suggest that XPB and XPD helicases differentially regulate the anchoring of CAK to core TFIIH during damage verification step of NER. PMID:23083890

  2. Fatigue behavior of adhesively bonded joints

    NASA Technical Reports Server (NTRS)

    Mall, S.

    1983-01-01

    The fatigue damage mechanism of composite to composite adhesively bonded joints was characterized. The mechanics of the possible modes of fatigue damage propagation in these joints when subjected to constant amplitude cyclic mechanical loading were investigated. The possible failure modes in composite bonded joints may be cyclic debonding (i.e., progressive separation of the adhesive), interlaminar damage (delamination), adherend fatigue or a combination of these. Two composite systems - graphite/epoxy adhesively bonded to graphite/epoxy and Kevlar 49/epoxy adhesively bonded to Kevlar 49/epoxy were investigated. Both composite systems consisted of quasi-isotropic lay-ups, i.e., 0 deg/-45 deg/+45 deg/90 degs. The two adhesives, employed in the study were (1) EC 3445 with cure temperature of 250 F for secondary bonding and (2) FM 300 with cure temperature of 350 F for co-cure bonding.

  3. Monitoring Tensile Fatigue of Superelastic NiTi Wire in Liquids by Electrochemical Potential

    NASA Astrophysics Data System (ADS)

    Racek, Jan; Stora, Marc; Šittner, Petr; Heller, Luděk; Kopeček, Jaromir; Petrenec, Martin

    2015-06-01

    Fatigue of superelastic NiTi wires was investigated by cyclic tension in simulated biofluid. The state of the surface of the fatigued NiTi wire was monitored by following the evolution of the electrochemical open circuit potential (OCP) together with macroscopic stresses and strains. The ceramic TiO2 oxide layer on the NiTi wire surface cannot withstand the large transformation strain and fractures in the first cycle. Based on the analysis of the results of in situ OCP experiments and SEM observation of cracks, it is claimed that the cycled wire surface develops mechanochemical reactions at the NiTi/liquid interface leading to cumulative generation of hydrogen, uptake of the hydrogen by the NiTi matrix, local loss of the matrix strength, crack transfer into the NiTi matrix, accelerated crack growth, and ultimately to the brittle fracture of the wire. Fatigue degradation is thus claimed to originate from the mechanochemical processes occurring at the excessively deforming surface not from the accumulation of defects due to energy dissipative bulk deformation processes. Ironically, combination of the two exciting properties of NiTi—superelasticity due to martensitic transformation and biocompatibility due to the protective TiO2 surface oxide layer—leads to excessive fatigue damage during cyclic mechanical loading in biofluids.

  4. Effect of moisture on the fatigue behavior of graphite/epoxy composite laminates

    NASA Technical Reports Server (NTRS)

    Ramani, S. V.; Nelson, H. G.

    1979-01-01

    The form of the moisture distribution in the specimen (gradient and flat profile) was considered to establish the influence of accelerated moisture conditioning on fatigue behavior. For the gradient specimens having an average moisture content of 1.4 percent, fatigue life was reduced by a factor of 8 at all stress levels investigated. Corresponding reduction in fatigue life for the flat moisture profile specimens at the same average moisture content was comparatively smaller, being about a factor of 5 from the value in dry specimens. X-ray radiographic analysis of damage accumulation in compression-compression fatigue revealed interlaminar cracking to be the dominant mode of failure responsible for the observed enhanced cyclic degradation of moisture-conditioned specimens. This finding was corroborated by the observed systematic reduction in interlaminar shear strength as a function of moisture content, which, in turn, increased the propensity for delamination under cyclic compressive loads. Residual strength measurements on cycled specimens indicated significant strength reductions at long lives, particularly in moisture conditioned specimens.

  5. Fatigue crack layer propagation in silicon-iron

    NASA Technical Reports Server (NTRS)

    Birol, Y.; Welsch, G.; Chudnovsky, A.

    1986-01-01

    Fatigue crack propagation in metal is almost always accompanied by plastic deformation unless conditions strongly favor brittle fracture. The analysis of the plastic zone is crucial to the understanding of crack propagation behavior as it governs the crack growth kinetics. This research was undertaken to study the fatigue crack propagation in a silicon iron alloy. Kinetic and plasticity aspects of fatigue crack propagation in the alloy were obtained, including the characterization of damage evolution.

  6. Energy release, beam attenuation radiation damage, gas production and accumulation of long-lived activity in Pb, Pb-Bi and Hg targets

    SciTech Connect

    Shubin, Yu.N.

    1996-06-01

    The calculation and analysis of the nuclei concentrations and long-lived residual radioactivity accumulated in Pb, Pb-Bi and Hg targets irradiated by 800 MeV, 30 mA proton beam have been performed. The dominating components to the total radioactivity of radionuclides resulting from fission and spallation reactions and radiative capture by both target nuclei and accumulated radioactive nuclei for various irradiation and cooling times were analyzed. The estimations of spectral component contributions of neutron and proton fluxes to the accumulated activity were carried out. The contributions of fission products to the targets activity and partial activities of main long-lived fission products to the targets activity and partial activities of main long-lived fission products were evaluated. The accumulation of Po isotopes due to reactions induced by secondary alpha-particles were found to be important for the Pb target as compared with two-step radiative capture. The production of Tritium in the targets and its contribution to the total targets activity was considered in detail. It is found that total activities of both targets are close to one another.

  7. Fatigue behavior and recommended design rules for an automotive composite

    SciTech Connect

    Corum, J.M.; Battiste, R.L.; Ruggles, M.B.

    1998-11-01

    Fatigue curves (stress vs cycles to failure) were generated under a variety of conditions (temperatures, fluid environments, mean stresses, block loadings) for a candidate automotive structural composite. The results were used to (1) develop observations regarding basic fatigue behavioral characteristics and (2) establish fatigue design rules. The composite was a structural reaction injection-molded polyurethane reinforced with continuous strand, swirl-mat E-glass fibers. Tensile fatigue tests on specimens from a single plaque at {minus}40 F, room temperature, and 250 F provided the basic behavioral characteristics. It was found that when stress was normalized by the at-temperature ultimate tensile strength, the fatigue curves at the three temperatures collapsed into a single master curve. An assessment of the individual stress-strain loops throughout each test showed a progressive loss in stiffness and an increase in permanent strain, both of which are indicative of increasing damage. Fatigue tests on specimens from several plaques were used to develop a design fatigue curve, which was established by using a reduction factor of 20 on average cycles to failure. This factor assures that the stiffness loss during the design life is no greater than 10 percent. Fatigue reduction factors were established to account for various fluids. Reversed stress fatigue tests allowed a mean stress rule to be validated, and block loading tests were used to demonstrate the adequacy of Miner`s rule for cumulative fatigue damage.

  8. The C-terminal region of Rad52 is essential for Rad52 nuclear and nucleolar localization, and accumulation at DNA damage sites immediately after irradiation

    SciTech Connect

    Koike, Manabu; Yutoku, Yasutomo; Koike, Aki

    2013-05-31

    Highlights: •Rad52 might play a key role in the repair of DSB immediately after irradiation. •EYFP-Rad52 accumulates rapidly at DSB sites and colocalizes with Ku80. •Accumulation of Rad52 at DSB sites is independent of the core NHEJ factors. •Localization and recruitment of Rad52 to DSB sites are dependent on the Rad52 CTR. •Basic amino acids in Rad52 CTR are highly conserved among vertebrate species. -- Abstract: Rad52 plays essential roles in homologous recombination (HR) and repair of DNA double-strand breaks (DSBs) in Saccharomyces cerevisiae. However, in vertebrates, knockouts of the Rad52 gene show no hypersensitivity to agents that induce DSBs. Rad52 localizes in the nucleus and forms foci at a late stage following irradiation. Ku70 and Ku80, which play an essential role in nonhomologous DNA-end-joining (NHEJ), are essential for the accumulation of other core NHEJ factors, e.g., XRCC4, and a HR-related factor, e.g., BRCA1. Here, we show that the subcellular localization of EYFP-Rad52(1–418) changes dynamically during the cell cycle. In addition, EYFP-Rad52(1–418) accumulates rapidly at microirradiated sites and colocalizes with the DSB sensor protein Ku80. Moreover, the accumulation of EYFP-Rad52(1–418) at DSB sites is independent of the core NHEJ factors, i.e., Ku80 and XRCC4. Furthermore, we observed that EYFP-Rad52(1–418) localizes in nucleoli in CHO-K1 cells and XRCC4-deficient cells, but not in Ku80-deficient cells. We also found that Rad52 nuclear localization, nucleolar localization, and accumulation at DSB sites are dependent on eight amino acids (411–418) at the end of the C-terminal region of Rad52 (Rad52 CTR). Furthermore, basic amino acids on Rad52 CTR are highly conserved among mammalian, avian, and fish homologues, suggesting that Rad52 CTR is important for the regulation and function of Rad52 in vertebrates. These findings also suggest that the mechanism underlying the regulation of subcellular localization of Rad52 is

  9. Mechanisms of High-Temperature Fatigue Failure in Alloy 800H

    NASA Technical Reports Server (NTRS)

    BhanuSankaraRao, K.; Schuster, H.; Halford, G. R.

    1996-01-01

    The damage mechanisms influencing the axial strain-controlled Low-Cycle Fatigue (LCF) behavior of alloy 800H at 850 C have been evaluated under conditions of equal tension/compression ramp rates (Fast-Fast (F-F): 4 X 10(sup -3)/s and Slow-Slow (S-S): 4 X 10(sup -5)/s) and asymmetrical ramp rates (Fast-Slow (F-S): 4 x 10(sup -3)/s / 4 X 10(sup -5/s and Slow-Fast (S-F): 4 X 10(sup -5) / 4 X 10(sup -3)/s) in tension and compression. The fatigue life, cyclic stress response, and fracture modes were significantly influenced by the waveform shape. The fatigue lives displayed by different loading conditions were in the following order: F-F greater than S-S greater than F-S greater than S-F. The fracture mode was dictated by the ramp rate adopted in the tensile direction. The fast ramp rate in the tensile direction led to the occurrence of transgranular crack initiation and propagation, whereas the slow ramp rate caused intergranular initiation and propagation. The time-dependent processes and their synergistic interactions, which were at the basis of observed changes in cyclic stress response and fatigue life, were identified. Oxidation, creep damage, dynamic strain aging, massive carbide precipitation, time-dependent creep deformation, and deformation ratcheting were among the several factors influencing cyclic life. Irrespective of the loading condition, the largest effect on life was exerted by oxidation processes. Deformation ratcheting had its greatest influence on life under asymmetrical loading conditions. Creep damage accumulated the greatest amount during the slow tensile ramp under S-F conditions.

  10. a Study on the Fretting Fatigue Life of Zircaloy Alloys

    NASA Astrophysics Data System (ADS)

    Kwon, Jae-Do; Park, Dae-Kyu; Woo, Seung-Wan; Chai, Young-Suck

    Studies on the strength and fatigue life of machines and structures have been conducted in accordance with the development of modern industries. In particular, fine and repetitive cyclic damage occurring in contact regions has been known to have an impact on fretting fatigue fractures. The main component of zircaloy alloy is Zr, and it possesses good mechanical characteristics at high temperatures. This alloy is used in the fuel rod material of nuclear power plants because of its excellent resistance. In this paper, the effect of the fretting damage on the fatigue behavior of the zircaloy alloy is studied. Further, various types of mechanical tests such as tension and plain fatigue tests are performed. Fretting fatigue tests are performed with a flat-flat contact configuration using a bridge-type contact pad and plate-type specimen. Through these experiments, it is found that the fretting fatigue strength decreases by about 80% as compared to the plain fatigue strength. Oblique cracks are observed in the initial stage of the fretting fatigue, in which damaged areas are found. These results can be used as the basic data for the structural integrity evaluation of corrosion-resisting alloys considering the fretting damages.

  11. Low cycle fatigue

    NASA Technical Reports Server (NTRS)

    Solomon, H. D. (Editor); Kaisand, L. R. (Editor); Halford, G. R. (Editor); Leis, B. N. (Editor)

    1988-01-01

    The papers contained in this volume focus on various aspects of low cycle fatigue, including cyclic deformation, crack propagation, high-temperature low cycle fatigue, microstructural defects, multiaxial and variable amplitude loading, and life prediction. Papers are presented on the low cycle fatigue of some aluminum alloys, prediction of crack growth under creep-fatigue loading conditions, high-temperature low cycle fatigue behavior and lifetime prediction of a nickel-base ODS alloy, and an integrated approach to creep-fatigue life prediction. Other topics discussed include thermal fatigue testing of coated monocrystalline superalloys, low cycle fatigue of Al-Mg-Si alloys, and the effect of superimposed stresses at high frequency on low cycle fatigue.

  12. A crystal plasticity based methodology for modeling fatigue crack initiation and estimating material coefficients to predict fatigue crack initiation life at micro, nano and macro scales

    NASA Astrophysics Data System (ADS)

    Voothaluru, Rohit

    Fatigue failure is a dominant mechanism that governs the failure of components and structures in many engineering applications. In conventional engineering applications due to the design specifications, a significant proportion of the fatigue life is spent in the crack initiation phase. In spite of the large number of works addressing fatigue life modeling, the problem of modeling crack initiation life still remains a major challenge. In this work, a novel computational methodology based upon crystal plasticity formulations has been developed to predict crack initiation life at macro, micro and nano length scales. The crystal plasticity based constitutive model has been employed to model the micromechanical deformation and damage accumulation under cyclic loading in polycrystalline metals. This work provides a first of its kind, fundamental basis for employing crystal plasticity formulations for evaluating a quantifiable estimate of fatigue crack initiation life. A semi-empirical energy based fatigue crack initiation criterion s employed to allow for accurate modeling of the underlying microstructural phenomenon leading to the initiation of cracks at different material length scales. The results of the fatigue crack initiation life prediction in case of polycrystalline metals such as Copper and Nickel demonstrated that the crack initiation life prediction using the proposed methodology yielded an improvement of more than 30% in comparison to the existing continuum methodologies for fatigue crack initiation prediction and more than 80% improvement compared to the existing analytical models. The computational methodology developed in this work also provides a first of its kind technique to evaluate the fatigue crack initiation coefficient in the form of energy dissipation coefficient that can be used at varying length scales. The methodology and the computational framework proposed in this work, are developed such that experimental inputs are used to improve

  13. Fatigue during intermittent-sprint exercise.

    PubMed

    Bishop, David J

    2012-09-01

    1. There is a reversible decline in force production by muscles when they are contracting at or near their maximum capacity. The task-dependent nature of fatigue means that the mechanisms of fatigue may differ between different types of contractions. This paper examines how fatigue manifests during whole-body, intermittent-sprint exercise and discusses the potential muscular and neural mechanisms that underpin this fatigue. 2. Fatigue is defined as a reversible, exercise-induced reduction in maximal power output (e.g. during cycling exercise) or speed (e.g. during running exercise), even though the task can be continued. 3. The small changes in surface electromyogram (EMG), along with a lack of change in voluntary muscle activation (estimated from both percutaneous motor nerve stimulations and trans-cranial magnetic stimulation), indicate that there is little change in neural drive to the muscles following intermittent-sprint exercise. This, along with the observation that the decrease in EMG is much less than that which would be predicted from the decrease in power output, suggests that peripheral mechanisms are the predominant cause of fatigue during intermittent-sprint exercise. 4. At the muscle level, limitations in energy supply, including phosphocreatine hydrolysis and the degree of reliance on anaerobic glycolysis and oxidative metabolism, and the intramuscular accumulation of metabolic by-products, such as hydrogen ions, emerge as key factors responsible for fatigue.

  14. [The influence of genotype on the effects of impact damage and light exposure on the accumulation of glycoalkaloids in potato tubers].

    PubMed

    Frydecka-Mazurczyk, A; Zgórska, K

    2001-01-01

    The experiment was carried out in 1999 with 24 Polish and 23 West European cultivars of potato tubers. The tubers were analysed for TGA content after 3 months of dark storage conditions in store-house (8 degrees C). 20 tubers of every cultivar were damaged and placed for a week to dark and under 15 W fluorescent lamps--13 mumol m-2 sec(-1)--equivalent to light in supermarkets. Glycoalkaloids content was determined with Bergers (1980) method. Tubers which had been damaged and exposed for a week to fluorescent light had two fold higher levels of TGA, than undamaged tubers stored at dark. It was found that content of glycoalkaloids in potato tubers and the increase of TGA content due to damage and light exposure depended on genotype. It was higher in varieties with higher level of these compounds, for example: Peppo, Albatros, Columbus and Santana (above 200 mg/kg fresh weight)--tab. I. The smaller increase occurred in tubers of varieties with low content of TGA: Polish--Irga, Grot, Baszta, Tokaj and West European--Satina, Ditta Gloria, Victoria--tab. I, II. Therefore it is important to breed new cultivars with low content of TGA.

  15. A Predictive Framework for Thermomechanical Fatigue Life of High Silicon Molybdenum Ductile Cast Iron Based on Considerations of Strain Energy Dissipation

    NASA Astrophysics Data System (ADS)

    Avery, Katherine R.

    Isothermal low cycle fatigue (LCF) and anisothermal thermomechanical fatigue (TMF) tests were conducted on a high silicon molybdenum (HiSiMo) cast iron for temperatures up to 1073K. LCF and out-of-phase (OP) TMF lives were significantly reduced when the temperature was near 673K due to an embrittlement phenomenon which decreases the ductility of HiSiMo at this temperature. In this case, intergranular fracture was predominant, and magnesium was observed at the fracture surface. When the thermal cycle did not include 673K, the failure mode was predominantly transgranular, and magnesium was not present on the fracture surface. The in-phase (IP) TMF lives were unaffected when the thermal cycle included 673K, and the predominant failure mode was found to be transgranular fracture, regardless of the temperature. No magnesium was present on the IP TMF fracture surfaces. Thus, the embrittlement phenomenon was found to contribute to fatigue damage only when the temperature was near 673K and a tensile stress was present. To account for the temperature- and stress-dependence of the embrittlement phenomenon on the TMF life of HiSiMo cast iron, an original model based on the cyclic inelastic energy dissipation is proposed which accounts for temperature-dependent differences in the rate of fatigue damage accumulation in tension and compression. The proposed model has few empirical parameters. Despite the simplicity of the model, the predicted fatigue life shows good agreement with more than 130 uniaxial low cycle and thermomechanical fatigue tests, cyclic creep tests, and tests conducted at slow strain rates and with hold times. The proposed model was implemented in a multiaxial formulation and applied to the fatigue life prediction of an exhaust manifold subjected to severe thermal cycles. The simulation results show good agreement with the failure locations and number of cycles to failure observed in a component-level experiment.

  16. Prestraining and Its Influence on Subsequent Fatigue Life

    NASA Technical Reports Server (NTRS)

    Halford, Gary R.; Mcgaw, Michael A.; Kalluri, Sreeramesh

    1995-01-01

    An experimental program was conducted to study the damaging effects of tensile and compressive prestrains on the fatigue life of nickel-base, Inconel 718 superalloy at room temperature. To establish baseline fatigue behavior, virgin specimens with a solid uniform gage section were fatigued to failure under fully-reversed strain-control. Additional specimens were prestrained to 2 percent, 5 percent, and 10 percent (engineering strains) in the tensile direction and to 2 percent (engineering strain) in the compressive direction under stroke-control, and were subsequently fatigued to failure under fully-reversed strain-control. Experimental results are compared with estimates of remaining fatigue lives (after prestraining) using three life prediction approaches: (1) the Linear Damage Rule; (2) the Linear Strain and Life Fraction Rule; and (3) the nonlinear Damage Curve Approach. The Smith-Watson-Topper parameter was used to estimate fatigue lives in the presence of mean stresses. Among the cumulative damage rules investigated, best remaining fatigue life predictions were obtained with the nonlinear Damage Curve Approach.

  17. Property Evaluation and Damage Evolution of Environmental Barrier Coatings and Environmental Barrier Coated SiC/SiC Ceramic Matrix Composite Sub-Elements

    NASA Technical Reports Server (NTRS)

    Zhu, Dongming; Halbig, Michael; Jaskowiak, Martha; Hurst, Janet; Bhatt, Ram; Fox, Dennis S.

    2014-01-01

    This paper describes recent development of environmental barrier coatings on SiC/SiC ceramic matrix composites. The creep and fatigue behavior at aggressive long-term high temperature conditions have been evaluated and highlighted. Thermal conductivity and high thermal gradient cyclic durability of environmental barrier coatings have been evaluated. The damage accumulation and complex stress-strain behavior environmental barrier coatings on SiCSiC ceramic matrix composite turbine airfoil subelements during the thermal cyclic and fatigue testing of have been also reported.

  18. Simulation of thermomechanical fatigue in solder joints

    SciTech Connect

    Fang, H.E.; Porter, V.L.; Fye, R.M.; Holm, E.A.

    1997-12-31

    Thermomechanical fatigue (TMF) is a very complex phenomenon in electronic component systems and has been identified as one prominent degradation mechanism for surface mount solder joints in the stockpile. In order to precisely predict the TMF-related effects on the reliability of electronic components in weapons, a multi-level simulation methodology is being developed at Sandia National Laboratories. This methodology links simulation codes of continuum mechanics (JAS3D), microstructural mechanics (GLAD), and microstructural evolution (PARGRAIN) to treat the disparate length scales that exist between the macroscopic response of the component and the microstructural changes occurring in its constituent materials. JAS3D is used to predict strain/temperature distributions in the component due to environmental variable fluctuations. GLAD identifies damage initiation and accumulation in detail based on the spatial information provided by JAS3D. PARGRAIN simulates the changes of material microstructure, such as the heterogeneous coarsening in Sn-Pb solder, when the component`s service environment varies.

  19. Cyclic fatigue behavior of cross-woven C/SiC composite

    SciTech Connect

    Wang, M.; Laird, C.

    1994-12-31

    Tension-tension fatigue experiments were conducted on CVI processed cross-woven Tension-tension fatigue experiments C/SiC composites. A S-N curve was obtained for this composite system, which defines a ``fatigue endurance`` in the range of 320 MPa 340 MPa (80%--85% of tensile strength) for run out cycles of 10{sup 6}. The behaviors of elapsed strains vs. cycles for the fatigue failed and run out samples are found to differ systematically, indicating that the elapsed strain is an appropriate parameter for describing fatigue damage. The total elapsed strains of the fatigue failed samples are in the range of 0.65%--O.75% which is characteristic of the cross woven C/SiC composite. The residual monotonic strength of the fatigue survived samples increased about 10% to 20% above the average monotonic tensile strength. Mechanisms for these 9 processes are discussed on the basis of fatigue damage development.

  20. UV-induced histone H2AX phosphorylation and DNA damage related proteins accumulate and persist in nucleotide excision repair-deficient XP-B cells.

    PubMed

    Oh, Kyu-Seon; Bustin, Michael; Mazur, Sharlyn J; Appella, Ettore; Kraemer, Kenneth H

    2011-01-01

    DNA double strand breaks (DSB) may be caused by ionizing radiation. In contrast, UV exposure forms dipyrimidine photoproducts and is not considered an inducer of DSB. We found that uniform or localized UV treatment induced phosphorylation of the DNA damage related (DDR) proteins H2AX, ATM and NBS1 and co-localization of γ-H2AX with the DDR proteins p-ATM, p-NBS1, Rad51 and FANCD2 that persisted for about 6h in normal human fibroblasts. This post-UV phosphorylation was observed in the absence of nucleotide excision repair (NER), since NER deficient XP-B cells (lacking functional XPB DNA repair helicase) and global genome repair-deficient rodent cells also showed phosphorylation and localization of these DDR proteins. Resolution of the DDR proteins was dependent on NER, since they persisted for 24h in the XP-B cells. In the normal and XP-B cells p53 and p21 was detected at 6h and 24h but Mdm2 was not induced in the XP-B cells. Post-UV induction of Wip1 phosphatase was detected in the normal cells but not in the XP-B cells. DNA DSB were detected with a neutral comet assay at 6h and 24h post-UV in the normal and XP-B cells. These results indicate that UV damage can activate the DDR pathway in the absence of NER. However, a later step in DNA damage processing involving induction of Wip1 and resolution of DDR proteins was not observed in the absence of NER.

  1. Accumulate-Repeat-Accumulate-Accumulate-Codes

    NASA Technical Reports Server (NTRS)

    Divsalar, Dariush; Dolinar, Sam; Thorpe, Jeremy

    2004-01-01

    Inspired by recently proposed Accumulate-Repeat-Accumulate (ARA) codes [15], in this paper we propose a channel coding scheme called Accumulate-Repeat-Accumulate-Accumulate (ARAA) codes. These codes can be seen as serial turbo-like codes or as a subclass of Low Density Parity Check (LDPC) codes, and they have a projected graph or protograph representation; this allows for a high-speed iterative decoder implementation using belief propagation. An ARAA code can be viewed as a precoded Repeat-and-Accumulate (RA) code with puncturing in concatenation with another accumulator, where simply an accumulator is chosen as the precoder; thus ARAA codes have a very fast encoder structure. Using density evolution on their associated protographs, we find examples of rate-lJ2 ARAA codes with maximum variable node degree 4 for which a minimum bit-SNR as low as 0.21 dB from the channel capacity limit can be achieved as the block size goes to infinity. Such a low threshold cannot be achieved by RA or Irregular RA (IRA) or unstructured irregular LDPC codes with the same constraint on the maximum variable node degree. Furthermore by puncturing the accumulators we can construct families of higher rate ARAA codes with thresholds that stay close to their respective channel capacity thresholds uniformly. Iterative decoding simulation results show comparable performance with the best-known LDPC codes but with very low error floor even at moderate block sizes.

  2. High-Cycle Fatigue Behavior of a Nicalon(tm)/Si-N-C Composite

    NASA Technical Reports Server (NTRS)

    Verrilli, Michael J.; Kalluri, Sreeramesh; Kantzos, Peter T.

    1999-01-01

    Elevated temperature, high-cycle fatigue behavior of a woven SiC/Si-N-C ceramic matrix composite system was investigated at 910 C. High frequency (100 Hz) fatigue tests were conducted in air on specimens machined from the composite system, A power-law type fatigue life relationship adequately characterized the high-cycle fatigue data generated in the study. Post failure fractographic and metallographic studies were performed to document the fatigue crack initiation regions and damage mechanisms in the composite system. Fatigue cracks initiated primarily from the corners of the specimens and propagated along the 90 degree fiber tows.

  3. Understanding impact-induced fatigue failure

    NASA Astrophysics Data System (ADS)

    Boyce, Brad Lee

    2001-07-01

    Aircraft turbine engines are susceptible to the ingestion of debris, leading to impact-damage sites on engine components, known as Foreign Object Damage (FOD). Under the influence of fatigue loading conditions, these damage sites can reduce the service lifetime of the component by several orders of magnitude and result in a catastrophic, unpredictable failure. The current study approaches this problem by evaluating the features that differentiate impact-induced fatigue behavior from normal undamaged fatigue behavior in a Ti-6A1-4V alloy typically used in the FOD-susceptible front stage of the engine. More specifically, the study focuses on the experimental determination of the local residual stress fields about sites of impact damage using spatially-resolved synchrotron x-ray diffraction. These results are used to evaluate the validity of recently developed numerical models, and provide insight into the limitations of such models. Moreover, the residual stress distributions are used to interpret fatigue behavior, where the initial residual stress state alone is shown to be a misleading metric for crack formation. The study shows that a more thorough evaluation of the impact-induced fatigue behavior also requires knowledge of the relaxation of the residual stress state during fatigue loading, as well as additional factors such as impact-induced microcracking, and geometric stress concentration. These factors are all combined into a fracture-mechanics based design framework to create a more complete understanding of the conditions for failure, and more importantly the multidimensional threshold condition, below which failure will not occur.

  4. Statistical optimisation techniques in fatigue signal editing problem

    SciTech Connect

    Nopiah, Z. M.; Osman, M. H.; Baharin, N.; Abdullah, S.

    2015-02-03

    Success in fatigue signal editing is determined by the level of length reduction without compromising statistical constraints. A great reduction rate can be achieved by removing small amplitude cycles from the recorded signal. The long recorded signal sometimes renders the cycle-to-cycle editing process daunting. This has encouraged researchers to focus on the segment-based approach. This paper discusses joint application of the Running Damage Extraction (RDE) technique and single constrained Genetic Algorithm (GA) in fatigue signal editing optimisation.. In the first section, the RDE technique is used to restructure and summarise the fatigue strain. This technique combines the overlapping window and fatigue strain-life models. It is designed to identify and isolate the fatigue events that exist in the variable amplitude strain data into different segments whereby the retention of statistical parameters and the vibration energy are considered. In the second section, the fatigue data editing problem is formulated as a constrained single optimisation problem that can be solved using GA method. The GA produces the shortest edited fatigue signal by selecting appropriate segments from a pool of labelling segments. Challenges arise due to constraints on the segment selection by deviation level over three signal properties, namely cumulative fatigue damage, root mean square and kurtosis values. Experimental results over several case studies show that the idea of solving fatigue signal editing within a framework of optimisation is effective and automatic, and that the GA is robust for constrained segment selection.

  5. Fatigue Behavior of a Third Generation PM Disk Superalloy

    NASA Technical Reports Server (NTRS)

    Gayda, John; Gabb, Timothy P.

    2008-01-01

    The fatigue behavior of a 3rd generation PM disk alloy, LSHR, was studied at 1300 F. Tensile, creep, and fatigue tests were run on smooth and notched (Kt = 2) bars under a variety of conditions. Analysis of smooth bar fatigue data, run under strain and load control with R ratios of 0 and -1, showed that a stress based Smith-Watson-Topper approach could collapse the data set. While the tensile and creep data showed substantial notch strengthening at 1300 F, the fatigue data showed a life deficit for the notch specimens. A viscoplastic finite element model, which accounted for stress relaxation at the notch tip, provided the best correlation between the notched and smooth bar behavior, although the fatigue data was not fully rationalized based on this simplified viscoplastic model of the stresses at the notch tip.Inclusion of a 90 sec dwell at peak load was found to dramatically decrease notch fatigue life. This result was shown to be consistent with a simple linear creep-fatigue damage rule, where creep damage dominated at low stresses and fatigue damage was more prevalent at higher stresses.

  6. [Fatigue and depression].

    PubMed

    Hell, Daniel

    2015-04-22

    Fatigue is characterised in an overview of the literature as a specific phenomenon of depression. Its differential diagnosis is discussed. Distinctions and correspondences to burnout are elaborated. Fatigue is not an obligatory symptom of depressive episodes, although it can contribute to depressive developments. The importance of fatigue in depressive episodes and its therapy is shown with the help of a circular model of depression.

  7. Fatigue of composites

    NASA Technical Reports Server (NTRS)

    Salkind, M. J.

    1972-01-01

    The failure mechanisms in the fatigue of composite materials are analyzed in terms of the requirements for designing fatigue-critical composite structures. Fiber reinforced polymers, fiber reinforced metals, fatigue of composite structures, and composite design considerations are discussed. It is concluded that composite materials offer the engineer the opportunity for tailoring stiffness in different directions for designing dynamic components.

  8. Accumulate-Repeat-Accumulate-Accumulate Codes

    NASA Technical Reports Server (NTRS)

    Divsalar, Dariush; Dolinar, Samuel; Thorpe, Jeremy

    2007-01-01

    Accumulate-repeat-accumulate-accumulate (ARAA) codes have been proposed, inspired by the recently proposed accumulate-repeat-accumulate (ARA) codes. These are error-correcting codes suitable for use in a variety of wireless data-communication systems that include noisy channels. ARAA codes can be regarded as serial turbolike codes or as a subclass of low-density parity-check (LDPC) codes, and, like ARA codes they have projected graph or protograph representations; these characteristics make it possible to design high-speed iterative decoders that utilize belief-propagation algorithms. The objective in proposing ARAA codes as a subclass of ARA codes was to enhance the error-floor performance of ARA codes while maintaining simple encoding structures and low maximum variable node degree.

  9. High temperature tension-compression fatigue behavior of a tungsten copper composite

    NASA Technical Reports Server (NTRS)

    Verrilli, Michael J.; Gabb, Timothy P.

    1990-01-01

    The high temperature fatigue of a (O)12 tungsten fiber reinforced copper matrix composite was investigated. Specimens having fiber volume percentages of 10 and 36 were fatigued under fully-reversed, strain-controlled conditions at both 260 and 560 C. The fatigue life was found to be independent of fiber volume fraction because fatigue damage preferentially occurred in the matrix. Also, the composite fatigue lives were shorter at 560 C as compared to 260 C due to changes in mode of matrix failure. On a total strain basis, the fatigue life of the composite at 560 C was the same as the life of unreinforced copper, indicating that the presence of the fibers did not degrade the fatigue resistance of the copper matrix in this composite system. Comparison of strain-controlled fatigue data to previously-generated load-controlled data revealed that the strain-controlled fatigue lives were longer because of mean strain and mean stress effects.

  10. Low cycle fatigue behavior of aluminum/stainless steel composites

    NASA Technical Reports Server (NTRS)

    Bhagat, R. B.

    1983-01-01

    Composites consisting of an aluminum matrix reinforced with various volume fractions of stainless steel wire were fabricated by hot die pressing under various conditions of temperature, time, and pressure. The composites were tested in plane bending to complete fracture under cycle loading, and the results were analyzed on a computer to obtain a statistically valid mathematical relationship between the low-cycle fatigue life and the fiber volume fraction of the composite. The fractured surfaces of the composites were examined by scanning electron microscopy to identify the characteristic features of fatigue damage. Fatigue damage mechanisms are proposed and discussed.

  11. Model for the accumulation of solar wind radiation damage effects in lunar dust grains, based on recent results concerning implantation and erosion effects

    SciTech Connect

    Borg, J.; Bibring, J.P.; Cowsik, G.; Langevin, Y.; Maurette, M.

    1983-02-15

    In this paper we present our most recent results on ion implantation and erosion effects, intended to reproduce the superficial amorphous layers of radiation damage observed with a high voltage electron microscope on ..mu..m-sized grains extracted from the lunar regolith and which result from the exposure of the grains to the solar wind. We next outline theoretical computations which yield the thickness distribution of such amorphous layers as a function of the exposure time of the grains at the surface of the moon, the He/H ratio, and the speed distribution in the solar wind. From this model, the position of the peak in the solar wind speed distribution is the major parameter controlling the thickness of the amorphous layer.

  12. Curcumin and Ellagic acid synergistically induce ROS generation, DNA damage, p53 accumulation and apoptosis in HeLa cervical carcinoma cells.

    PubMed

    Kumar, Devbrat; Basu, Soumya; Parija, Lucy; Rout, Deeptimayee; Manna, Sanjeet; Dandapat, Jagneshwar; Debata, Priya Ranjan

    2016-07-01

    Cervical cancer and precancerous lesions of the cervix continue to be a global health issue, and the medication for the treatment for chronic HPV infection so far has not been effective. Potential anticancer and anti HPV activities of two known phytochemicals, Curcumin and Ellagic acid were evaluated in HeLa cervical cancer cells. Curcumin is a natural compound found in the root of Curcuma longa plant and Ellagic acid a polyphenol found in fruits of strawberries, raspberries and walnuts. The combination of Curcumin and Ellagic acid at various concentrations showed better anticancer properties than either of the drug when used alone as evidenced by MTT assay. Besides this, Curcumin and Ellagic acid also restore p53, induce ROS formation and DNA damage. Mechanistic study further indicated that Curcumin and Ellagic acid show anti-HPV activity as evidenced by decrease in the HPV E6 oncoprotein on HeLa cells. PMID:27261574

  13. Curcumin and Ellagic acid synergistically induce ROS generation, DNA damage, p53 accumulation and apoptosis in HeLa cervical carcinoma cells.

    PubMed

    Kumar, Devbrat; Basu, Soumya; Parija, Lucy; Rout, Deeptimayee; Manna, Sanjeet; Dandapat, Jagneshwar; Debata, Priya Ranjan

    2016-07-01

    Cervical cancer and precancerous lesions of the cervix continue to be a global health issue, and the medication for the treatment for chronic HPV infection so far has not been effective. Potential anticancer and anti HPV activities of two known phytochemicals, Curcumin and Ellagic acid were evaluated in HeLa cervical cancer cells. Curcumin is a natural compound found in the root of Curcuma longa plant and Ellagic acid a polyphenol found in fruits of strawberries, raspberries and walnuts. The combination of Curcumin and Ellagic acid at various concentrations showed better anticancer properties than either of the drug when used alone as evidenced by MTT assay. Besides this, Curcumin and Ellagic acid also restore p53, induce ROS formation and DNA damage. Mechanistic study further indicated that Curcumin and Ellagic acid show anti-HPV activity as evidenced by decrease in the HPV E6 oncoprotein on HeLa cells.

  14. German experiences in local fatigue monitoring

    SciTech Connect

    Abib, E.; Bergholz, S.; Rudolph, J.

    2012-07-01

    The ageing management of nuclear power plants (NPP) has gained an increasing importance in the last years. The reasons are mainly due to the international context of extending period of plants operation. Moreover, new scientific discoveries, such as the corrosive influence of the medium on the fatigue process (environmentally assisted fatigue - EAF) play an important role and influence the code development (ASME, EAF code cases). The fatigue damage process takes a central position in ageing mechanisms of components. It must be ensured through appropriate evidence that facilities are being operated under allowable boundary conditions. In the design phase of NPP, fatigue analyses are still based on theoretical considerations and empirical values, which are summarized in the design transient catalogue, necessary for licensing. These analyses aim at proving the admissibility of the loads in terms of stress and fatigue usage. These analyses will also provide the fatigue-relevant positions in the NPP and give a basis for future design improvements and optimization of operating modes. The design transients are in practice conservatively correlated with the real transients occurring during operation. Uncertainties reveal very conservative assumptions regarding forecast temperatures, temperature gradients and frequencies of events. During operation of the plant, it has to be recurrently proved, that the plant is being operated under designed boundary conditions. Moreover, operating signals are constantly acquired to enable a fatigue evaluation. For example, in Germany fatigue evaluation is based on decades of experience and regulatory requirements. The rule KTA 3201.4 [1] establishes the rules for qualified fatigue monitoring. The rule DIN 25475-3 [2] on fatigue monitoring systems is available in draft version. Experience shows that some significant differences occur between the design transients and the real occurred transients during plant operation. The reasons for it

  15. High temperature fatigue behavior of tungsten copper composites

    NASA Technical Reports Server (NTRS)

    Verrilli, M. J.; Kim, Y.-S.; Gabb, T. P.

    1990-01-01

    The present study investigates the high-temperature fatigue behavior of a 9-v/o tungsten fiber-reinforced copper matrix composite. Load-controlled isothermal fatigue at 260 and 560 C and thermomechanical fatigue (TMF) experiments, both in-phase and out-of-phase between 260 and 560 C, were performed. The stress-strain response under all conditions displayed considerable inelasticity. Strain ratchetting was observed during all the fatigue experiments. For the isothermal fatigue and in-phase TMF tests, the ratchetting was always in a tensile direction, continuing until failure. The ratchetting during the out-of-phase TMF test shifted from a tensile to a compressive direction. For all cases, the fatigue lives were found to be controlled by the damage of the copper matrix. On a stress basis, TMF loading substantially reduced lives relative to isothermal cycling.

  16. Compressive fatigue and fracture toughness behavior of injectable, settable bone cements.

    PubMed

    Harmata, Andrew J; Uppuganti, Sasidhar; Granke, Mathilde; Guelcher, Scott A; Nyman, Jeffry S

    2015-11-01

    Bone grafts used to repair weight-bearing tibial plateau fractures often experience cyclic loading, and there is a need for bone graft substitutes that prevent failure of fixation and subsequent morbidity. However, the specific mechanical properties required for resorbable grafts to optimize structural compatibility with native bone have yet to be established. While quasi-static tests are utilized to assess weight-bearing ability, compressive strength alone is a poor indicator of in vivo performance. In the present study, we investigated the effects of interfacial bonding on material properties under conditions that re-capitulate the cyclic loading associated with weight-bearing fractures. Dynamic compressive fatigue properties of polyurethane (PUR) composites made with either unmodified (U-) or polycaprolactone surface-modified (PCL-) 45S5 bioactive glass (BG) particles were compared to a commercially available calcium sulfate and phosphate-based (CaS/P) bone cement at physiologically relevant stresses (5-30 MPa). Fatigue resistance of PCL-BG/polymer composite was superior to that of the U-BG/polymer composite and the CaS/P cement at higher stress levels for each of the fatigue failure criteria, related to modulus, creep, and maximum displacement, and was comparable to human trabecular bone. Steady state creep and damage accumulation occurred during the fatigue life of the PCL-BG/polymer and CaS/P cement, whereas creep of U-BG/polymer primarily occurred at a low number of loading cycles. From crack propagation testing, fracture toughness or resistance to crack growth was significantly higher for the PCL-BG composite than for the other materials. Finally, the fatigue and fracture toughness properties were intermediate between those of trabecular and cortical bone. These findings highlight the potential of PCL-BG/polyurethane composites as weight-bearing bone grafts. PMID:26282077

  17. Fatigue in rheumatic diseases

    PubMed Central

    Sandıkçı, Sevinç Can; Özbalkan, Zeynep

    2015-01-01

    Fatigue is a common and important problem in many diseases including rheumatologic illnesses, and it has a negative impact on health-related quality of life. Fatigue is described as having an impact on multiple aspects of a patient’s life. There is a need for knowledge about causes of and treatments for fatigue to ensure that patient outcomes are improved. There are several effective treatment strategies available for fatigue including pharmacological and non-pharmacological therapies. We aim to provide an overview of fatigue in rheumatologic disorders and some recommendations on its optimal management. PMID:27708942

  18. Cognitive and Physical Fatigue Tasks Enhance Pain, Cognitive Fatigue and Physical Fatigue in People with Fibromyalgia

    PubMed Central

    Dailey, Dana L; Keffala, Valerie J; Sluka, Kathleen A

    2014-01-01

    Objective Fibromyalgia is a condition characterized by chronic widespread muscle pain and fatigue. The primary objective of this study was to determine if pain, perceived cognitive fatigue, and perceived physical fatigue were enhanced in participants with fibromyalgia compared to healthy controls during a cognitive fatigue task, a physical fatigue task and a dual fatigue task. Methods Twenty four people with fibromyalgia and 33 healthy controls completed pain, fatigue and function measures. A cognitive fatigue task (Controlled Oral Word Association Test) and physical fatigue task (Valpar peg test) were done individually and combined for a dual fatigue task. Resting pain, perceived cognitive fatigue and perceived physical fatigue were assessed during each task using visual analogue scales. Function was assessed with shoulder range of motion and grip. Results People with fibromyalgia had significantly higher increases in pain, cognitive fatigue and physical fatigue when compared to healthy controls after completion of a cognitive fatigue task, a physical fatigue task, or a dual fatigue task (p<0.01). People with fibromyalgia performed equivalently on measures of physical performance and cognitive performance on the physical and cognitive fatigue tasks, respectively. Conclusions These data show that people with fibromyalgia show larger increases in pain, perceived cognitive fatigue and perceived physical fatigue to both cognitive and physical fatigue tasks compared to healthy controls. The increases in pain and fatigue during cognitive and physical fatigue tasks could influence subject participation in daily activities and rehabilitation. PMID:25074583

  19. Organ Damage and Hepatic Lipid Accumulation in Carp (Cyprinus carpio L.) after Feed-Borne Exposure to the Mycotoxin, Deoxynivalenol (DON)

    PubMed Central

    Pietsch, Constanze; Schulz, Carsten; Rovira, Pere; Kloas, Werner; Burkhardt-Holm, Patricia

    2014-01-01

    Deoxynivalenol (DON) frequently contaminates animal feed, including fish feed used in aquaculture. This study intends to further investigate the effects of DON on carp (Cyprinus carpio L.) at concentrations representative for commercial fish feeds. Experimental feeding with 352, 619 or 953 μg DON kg−1 feed resulted in unaltered growth performance of fish during six weeks of experimentation, but increased lipid peroxidation was observed in liver, head kidney and spleen after feeding of fish with the highest DON concentration. These effects of DON were mostly reversible by two weeks of feeding the uncontaminated control diet. Histopathological scoring revealed increased liver damage in DON-treated fish, which persisted even after the recovery phase. At the highest DON concentration, significantly more fat, and consequently, increased energy content, was found in whole fish body homogenates. This suggests that DON affects nutrient metabolism in carp. Changes of lactate dehydrogenase (LDH) activity in kidneys and muscle and high lactate levels in serum indicate an effect of DON on anaerobic metabolism. Serum albumin was reduced by feeding the medium and a high dosage of DON, probably due to the ribotoxic action of DON. Thus, the present study provides evidence of the effects of DON on liver function and metabolism. PMID:24566729

  20. Organ damage and hepatic lipid accumulation in carp (Cyprinus carpio L.) after feed-borne exposure to the mycotoxin, deoxynivalenol (DON).

    PubMed

    Pietsch, Constanze; Schulz, Carsten; Rovira, Pere; Kloas, Werner; Burkhardt-Holm, Patricia

    2014-02-21

    Deoxynivalenol (DON) frequently contaminates animal feed, including fish feed used in aquaculture. This study intends to further investigate the effects of DON on carp (Cyprinus carpio L.) at concentrations representative for commercial fish feeds. Experimental feeding with 352, 619 or 953 μg DON kg(-1) feed resulted in unaltered growth performance of fish during six weeks of experimentation, but increased lipid peroxidation was observed in liver, head kidney and spleen after feeding of fish with the highest DON concentration. These effects of DON were mostly reversible by two weeks of feeding the uncontaminated control diet. Histopathological scoring revealed increased liver damage in DON-treated fish, which persisted even after the recovery phase. At the highest DON concentration, significantly more fat, and consequently, increased energy content, was found in whole fish body homogenates. This suggests that DON affects nutrient metabolism in carp. Changes of lactate dehydrogenase (LDH) activity in kidneys and muscle and high lactate levels in serum indicate an effect of DON on anaerobic metabolism. Serum albumin was reduced by feeding the medium and a high dosage of DON, probably due to the ribotoxic action of DON. Thus, the present study provides evidence of the effects of DON on liver function and metabolism.

  1. Low cycle fatigue and creep-fatigue behavior of Ni-based alloy 230 at 850 C

    SciTech Connect

    Chen, Xiang; Yang, Zhiqing; Sokolov, Mikhail A; ERDMAN III, DONALD L; Mo, Kun; Stubbins, James

    2013-01-01

    Strain-controlled low cycle fatigue (LCF) and creep-fatigue testing of Ni-based alloy 230 were carried out at 850 C. The material creep-fatigue life decreased compared with its low cycle fatigue life at the same total strain range. Longer hold time at peak tensile strain further reduced the material creep-fatigue life. Based on the electron backscatter diffraction, a novel material deformation characterization method was applied, which revealed that in low cycle fatigue testing as the total strain range increased, the deformation was segregated to grain boundaries since the test temperature was higher than the material equicohesive temperature and grain boundaries became weaker regions compared with grains. Creep-fatigue tests enhanced the localized deformation, resulting in material interior intergranular cracking, and accelerated material damage. Precipitation in alloy 230 helped slip dispersion, favorable for fatigue property, but grain boundary cellular precipitates formed after material exposure to the elevated temperature had a deleterious effect on the material low cycle fatigue and creep-fatigue property.

  2. Fatigue handbook: Offshore steel structures

    SciTech Connect

    Almarnaess, A.

    1985-01-01

    The contents of this book are: Overview of Offshore Steel Structures; Loads on Ocean Structures; Fracture Mechanics As a Tool in Fatigue Analysis; Basic Fatigue Properties of Welded Joints; Significance of Defects; Improving the Fatigue Strength of Welded Joints; Effects of Marine Environment and Cathodic Protection on Fatigue of Structural Steels Fatigue of Tubular Joints; Unstable Fracture; Fatigue Life Calculations; and Fatigue in Building Codes Background and Applications.

  3. Lamb Wave Response of Fatigued Composite Samples

    NASA Technical Reports Server (NTRS)

    Seale, Michael; Smith, Barry T.; Prosser, William H.; Masters, John E.

    1994-01-01

    Composite materials are being more widely used today by aerospace, automotive, sports equipment, and a number of other commercial industries because of their advantages over conventional metals. Composites have a high strength-to-weight ratio and can be constructed to meet specific design needs. Composite structures are already in use in secondary parts of the Douglas MD-11 and are planned to be used in the new MD-12X. Plans also exist for their use in primary and secondary structures on the Boeing 777. Douglas proposed MD-XX may also incorporate composite materials into primary structures such as the wings and tail. Use of composites in these structures offers weight savings, corrosion resistance, and improved aerodynamics. Additionally, composites have been used to repair cracks in many B-1Bs where traditional repair techniques were not very effective. Plans have also been made to reinforce all of the remaining B-1s with composite materials. Verification of the structural integrity of composite components is needed to insure safe operation of these aerospace vehicles. One aspect of the use of these composites is their response to fatigue. To track this progression of fatigue in aerospace structures, a convenient method to nondestructively monitor this damage needs to be developed. Traditional NDE techniques used on metals are not easily adaptable to composites due to the inhomogeneous and anisotropic nature of these materials. Finding an effective means of nondestructively monitoring fatigue damage is extremely important to the safety and reliability of such structures. Lamb waves offer one method of evaluating these composite materials. As a material is fatigued, the modulus degrades. Since the Lamb wave velocity can be related to the modulus of the material, an effective tool can be developed to monitor fatigue damage in composites by measuring the velocity of these waves. In this work, preliminary studies have been conducted which monitor fatigue damage in

  4. Mild steel welding fume causes manganese accumulation and subtle neuroinflammatory changes but not overt neuronal damage in discrete brain regions of rats after short-term inhalation exposure.

    PubMed

    Antonini, James M; Sriram, Krishnan; Benkovic, Stanley A; Roberts, Jenny R; Stone, Samuel; Chen, Bean T; Schwegler-Berry, Diane; Jefferson, Amy M; Billig, Brenda K; Felton, Christopher M; Hammer, Mary Ann; Ma, Fang; Frazer, David G; O'Callaghan, James P; Miller, Diane B

    2009-11-01

    Serious questions have been raised by occupational health investigators regarding a possible causal association between neurological effects in welders and the presence of manganese (Mn) in welding fume. Male Sprague-Dawley rats were exposed by inhalation to 40 mg/m(3) of gas metal arc-mild steel (MS) welding fume for 3 h/day for 10 days. Generated fume was collected in the animal chamber during exposure, and particle size, composition, and morphology were characterized. At 1 day after the last exposure, metal deposition in different organ systems and neurological responses in dopaminergic brain regions were assessed in exposed animals. The welding particles were composed primarily of a complex of iron (Fe) and Mn and were arranged as chain-like aggregates with a significant number of particles in the nanometer size range. Mn was observed to translocate from the lungs to the kidney and specific brain regions (olfactory bulb, cortex, and cerebellum) after MS fume inhalation. In terms of neurological responses, short-term MS fume inhalation induced significant elevations in divalent metal ion transporter 1 (Dmt1) expression in striatum and midbrain and significant increases in expression of proinflammatory chemokines (Ccl2, Cxcl2) and cytokines (IL1beta, TNFalpha) in striatum. In addition, mRNA and protein expression of glial fibrillary acidic protein (GFAP) was significantly increased in striatum after MS fume exposure. However, the 10-day MS welding fume inhalation did not cause any changes in dopamine and its metabolites or GABA in dopaminergic brain regions nor did it produce overt neural cell damage as assessed by histopathology. In summary, short-term MS welding fume exposure led to translocation of Mn to specific brain regions and induced subtle changes in cell markers of neuroinflammatory and astrogliosis. The neurofunctional significance of these findings currently is being investigated in longer, more chronic welding fume exposure studies.

  5. Evaluation of 3-hydroxybutyrate as an enzyme-protective agent against heating and oxidative damage and its potential role in stress response of poly(3-hydroxybutyrate) accumulating cells.

    PubMed

    Obruca, Stanislav; Sedlacek, Petr; Mravec, Filip; Samek, Ota; Marova, Ivana

    2016-02-01

    Poly(3-hydroxybutyrate) (PHB) is a common carbon- and energy-storage compound simultaneously produced and degraded into its monomer 3-hydroxybutyrate (3HB) by numerous bacteria and Archae in a metabolic pathway called the PHB cycle. We investigated 3HB as a chemical chaperone capable of protecting model enzymes, namely lipase and lysozyme, from adverse effects of high temperature and oxidation. Heat-mediated denaturation of lipase in the presence or absence of 3HB was monitored by dynamic light scattering (DLS) revealing a significant protective effect of 3HB which increased as its concentration rose. Furthermore, when compared at the same molar concentration, 3HB showed a greater protective effect than the well-known chemical chaperones trehalose and hydroxyectoine. The higher protective effect of 3HB was also confirmed when employing differential scanning calorimetry (DSC) and lysozyme as a model enzyme. Furthermore, 3HB was capable of protecting lipase not only against thermal-mediated denaturation but also against oxidative damage by Cu(2+) and H2O2; its protection was higher than that of trehalose and comparable to that of hydroxyectoine. Taking into account that the PHB-producing strain Cupriavidus necator H16 reveals a 16.5-fold higher intracellular concentration than the PHB non-producing mutant C. necator PHB(-4), it might be expected that the functional PHB cycle might be responsible for maintaining a higher intracellular level of 3HB which, aside from other positive aspects of functional PHB metabolism, enhances stress resistance of bacterial strains capable of simultaneous PHB synthesis and mobilization. In addition, 3HB can be used in various applications and formulations as an efficient enzyme-stabilizing and enzyme-protecting additive. PMID:26590589

  6. High Potency of a Novel Resveratrol Derivative, 3,3',4,4'-Tetrahydroxy-trans-stilbene, against Ovarian Cancer Is Associated with an Oxidative Stress-Mediated Imbalance between DNA Damage Accumulation and Repair.

    PubMed

    Mikuła-Pietrasik, Justyna; Sosińska, Patrycja; Murias, Marek; Wierzchowski, Marcin; Brewińska-Olchowik, Marta; Piwocka, Katarzyna; Szpurek, Dariusz; Książek, Krzysztof

    2015-01-01

    We explored the effect of a new resveratrol (RVT) derivative, 3,3',4,4'-tetrahydroxy-trans-stilbene (3,3',4,4'-THS), on viability, apoptosis, proliferation, and senescence of three representative lines of ovarian cancer cells, that is, A2780, OVCAR-3, and SKOV-3, in vitro. In addition, the mechanistic aspects of 3,3',4,4'-THS activity, including cell redox homeostasis (the production of reactive oxygen species, activity of enzymatic antioxidants, and magnitude of DNA damage accumulation and repair), and the activity of caspases (3, 8, and 9) and p38 MAPK were examined. The study showed that 3,3',4,4'-THS affects cancer cell viability much more efficiently than its parent drug. This effect coincided with increased generation of reactive oxygen species, downregulated activity of superoxide dismutase and catalase, and excessive accumulation of 8-hydroxy-2'-deoxyguanosine and its insufficient repair due to decreased expression of DNA glycosylase I. Cytotoxicity elicited by 3,3',4,4'-THS was related to increased incidence of apoptosis, which was mediated by caspases 3 and 9. Moreover, 3,3',4,4'-THS inhibited cancer cell proliferation and accelerated senescence, which was accompanied by the activation of p38 MAPK. Collectively, our findings indicate that 3,3',4,4'-THS may constitute a valuable tool in the fight against ovarian malignancy and that the anticancer capabilities of this stilbene proceed in an oxidative stress-dependent mechanism. PMID:26229578

  7. Multiaxial fatigue low cycle fatigue testing

    NASA Technical Reports Server (NTRS)

    Zamrik, S. Y.

    1985-01-01

    Multiaxial testing methods are reviewed. Advantages and disadvantages of each type test is discussed. Significant multiaxial data available in the literature is analyzed. The yield theories are compared for multiaxial fatigue analysis.

  8. Cynanchum wilfordii Radix attenuates liver fat accumulation and damage by suppressing hepatic cyclooxygenase-2 and mitogen-activated protein kinase in mice fed with a high-fat and high-fructose diet.

    PubMed

    Jang, Seon-A; Lee, SungRyul; Sohn, Eun-Hwa; Yang, Jaehyuk; Park, Dae Won; Jeong, Yong Joon; Kim, Inhye; Kwon, Jung Eun; Song, Hae Seong; Cho, Young Mi; Meng, Xue; Koo, Hyun Jung; Kang, Se Chan

    2016-09-01

    Excessive consumption of fat and fructose augments the pathological progression of nonalcoholic fatty liver disease through hepatic fibrosis, inflammation, and hepatic de novo lipogenesis. We hypothesized that supplementation with Cynanchum wilfordii extract (CWE) decreases fat accumulation in the liver by suppressing cyclooxygenase-2 (COX-2), the nuclear translocation of nuclear factor κB (NF-κB), and p38 mitogen-activated protein kinase (MAPK). The beneficial effect of CWE was evaluated in a murine model of nonalcoholic fatty liver disease. Mice were fed either a normal diet or an atherogenic diet with fructose (ATHFR) in the presence or absence of CWE (50, 100, or 200 mg/kg; n=6/group). Treatment with ATHFR induced a hepatosplenomegaly-like condition (increased liver and spleen weight); this pathological change was attenuated in the presence of CWE. The ATHFR group exhibited impaired liver function, as evidenced by increased blood levels of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase, fat accumulation in the liver, and lipid profiles. Supplementation of CWE (100 and 200 mg/kg, P<.05) ameliorated these impaired liver functions. Atherogenic diet with fructose increased the protein levels of COX-2 and p38 MAPK, as well as the nuclear translocation of NF-κB. These signaling pathways, which are associated with the inflammatory response, were markedly suppressed after CWE treatment (100 and 200 mg/kg). In summary, CWE supplementation reduced high-fat and high-fructose diet-induced fat accumulation and damage in the liver by suppressing COX-2, NF-κB, and p38 MAPK. PMID:27632911

  9. Nerve damage from diabetes - self-care

    MedlinePlus

    ... and bloating Nausea, constipation, or diarrhea Swallowing problems Throwing up undigested food several hours after a meal Heart- ... are: Sudden fatigue Sweating Shortness of breath Nausea Vomiting Other symptoms of nerve damage are: Sexual problems. ...

  10. From monoscale to multiscale modeling of fatigue crack growth: Stress and energy density factor

    NASA Astrophysics Data System (ADS)

    Sih, G. C.

    2014-01-01

    The formalism of the earlier fatigue crack growth models is retained to account for multiscaling of the fatigue process that involves the creation of macrocracks from the accumulation of micro damage. The effects of at least two scales, say micro to macro, must be accounted for. The same data can thus be reinterpreted by the invariancy of the transitional stress intensity factors such that the microcracking and macrocracking data would lie on a straight line. The threshold associated with the sigmoid curve disappears. Scale segmentation is shown to be a necessity for addressing multiscale energy dissipative processes such as fatigue and creep. Path independency and energy release rate are monoscale criteria that can lead to unphysical results, violating the first principles. Application of monoscale failure or fracture criteria to nanomaterials is taking toll at the expense of manufacturing super strength and light materials and structural components. This brief view is offered in the spirit of much needed additional research for the reinforcement of materials by creating nanoscale interfaces with sustainable time in service. The step by step consideraton at the different scales may offer a better understanding of the test data and their limitations with reference to space and time.

  11. Interfacial cyclic fatigue of atomic-layer-deposited alumina coatings on silicon thin films.

    PubMed

    Baumert, Eva K; Pierron, Olivier N

    2013-07-10

    A microresonator-based interfacial fatigue testing technique was used to investigate the subcritical delamination of atomic-layer-deposited alumina coatings along the sidewalls of deep-reactive-ion-etched monocrystalline silicon thin films. This technique ensures loading conditions relevant to microelectromechanical system devices, including kHz testing frequency and fully reversed cyclic stresses. Four different coating thicknesses (4.2, 12.6, 25, and 50 nm) were investigated in two environments (30 °C, 50% relative humidity (RH) and 80 °C, 90% RH). Fatigue damage, in the form of channel cracks and delamination of the alumina coating, was found to accumulate slowly over more than 1 × 10(8) cycles. The average delamination rates increase with increasing energy release rate amplitude for delamination, modeled with a power law relationship. In the harsher environment, the rates are roughly 1 order of magnitude higher. Additionally, a few tests under static load were conducted for which no delamination (or crack growth) occurred, demonstrating that the governing interfacial fatigue mechanism is cycle-dependent.

  12. Creep-Fatigue Relationsihps in Electroactive Polymer Systems and Predicted Effects in an Actuator Design

    NASA Technical Reports Server (NTRS)

    Vinogradov, Aleksandra M.; Ihlefeld, Curtis M.; Henslee, Issac

    2009-01-01

    The paper concerns the time-dependent behavior of electroactive polymers (EAP) and their use in advanced intelligent structures for space exploration. Innovative actuator design for low weight and low power valves required in small plants planned for use on the moon for chemical analysis is discussed. It is shown that in-depth understanding of cyclic loading effects observed through accelerated creep rates due to creep-fatigue interaction in polymers is critical in terms of proper functioning of EAP based actuator devices. In the paper, an overview of experimental results concerning the creep properties and cyclic creep response of a thin film piezoelectric polymer polyvinylidene fluoride (PVDF) is presented. The development of a constitutive creep-fatigue interaction model to predict the durability and service life of electroactive polymers is discussed. A novel method is proposed to predict damage accumulation and fatigue life of polymers under oyclic loading conditions in the presence of creep. The study provides a basis for ongoing research initiatives at the NASA Kennedy Space Center in the pursuit of new technologies using EAP as active elements for lunar exploration systems.

  13. Dynamic Fatigue of ULE Glass

    NASA Technical Reports Server (NTRS)

    Tucker, Dennis S.; Nettles, Alan T.; Brantley, Lott W. (Technical Monitor)

    2001-01-01

    Ultra Low Expansion (ULE) glass is used in a number of applications which require a low thermal expansion coefficient. One such application is telescope mirror elements. An allowable stress can be calculated for this material based upon modulus of rupture data; however, this does not take into account the problem of delayed failure. Delayed failure, due to stress corrosion can significantly shorten the lifetime of a glass article. Knowledge of the factors governing the rate of subcritical flaw growth in a given environment enables the development of relations between lifetime, applied stress and failure probability for the material under study. Dynamic fatigue is one method of obtaining the necessary information to develop these relationships. In this study, the dynamic fatigue method was used to construct time-to-failure diagrams for both 230/270 ground and optically polished samples. The grinding and polishing process reduces the surface flaw size and subsurface damage, and relieves residual stress by removing materials with successively smaller grinding media. This resulted in an increase in the strength of the optic during the grinding and polishing sequence. There was also an increase in the lifetime due to grinding and polishing. It was found that using the fatigue parameters determined from the 230/270 grit surface are not significantly different from the optically polished values. Although the lower bound of the polished samples is more conservative, neither time-to-failure curves lie beyond the upper or lower bound of the confidence limits. Therefore, designers preferring conservative limits could use samples without residual stress present (polished samples) to determine the fatigue parameters and inert Weibull parameters from samples with the service condition surface, to determine time-to-failure of the optical element.

  14. Clinical neurophysiology of fatigue.

    PubMed

    Zwarts, M J; Bleijenberg, G; van Engelen, B G M

    2008-01-01

    Fatigue is a multidimensional concept covering both physiological and psychological aspects. Chronic fatigue is a typical symptom of diseases such as cancer, multiple sclerosis (MS), Parkinson's disease (PD) and cerebrovascular disorders but is also presented by people in whom no defined somatic disease has been established. If certain criteria are met, chronic fatigue syndrome can be diagnosed. The 4-item Abbreviated Fatigue Questionnaire allows the extent of the experienced fatigue to be assessed with a high degree of reliability and validity. Physiological fatigue has been well defined and originates in both the peripheral and central nervous system. The condition can be assessed by combining force and surface-EMG measurements (including frequency analyses and muscle-fibre conduction estimations), twitch interpolation, magnetic stimulation of the motor cortex and analysis of changes in the readiness potential. Fatigue is a well-known phenomenon in both central and peripheral neurological disorders. Examples of the former conditions are multiple sclerosis, Parkinson's disease and stroke. Although it seems to be a universal symptom of many brain disorders, the unique characteristics of the concomitant fatigue also point to a specific relationship with several of these syndromes. As regards neuromuscular disorders, fatigue has been reported in patients with post-polio syndrome, myasthenia gravis, Guillain-Barré syndrome, facioscapulohumeral dystrophy, myotonic dystrophy and hereditary motor and sensory neuropathy type-I. More than 60% of all neuromuscular patients suffer from severe fatigue, a prevalence resembling that of patients with MS. Except for several rare myopathies with specific metabolic derangements leading to exercise-induced muscle fatigue, most studies have not identified a prominent peripheral cause for the fatigue in this population. In contrast, the central activation of the diseased neuromuscular system is generally found to be suboptimal. The

  15. High-speed scanning ablation of dental hard tissues with a λ = 9.3 μm CO2 laser: adhesion, mechanical strength, heat accumulation, and peripheral thermal damage

    PubMed Central

    Nguyen, Daniel; Chang, Kwang; Hedayatollahnajafi, Saba; Staninec, Michal; Chan, Kenneth; Lee, Robert; Fried, Daniel

    2011-01-01

    CO2 lasers can be operated at high laser pulse repetition rates for the rapid and precise removal of dental decay. Excessive heat accumulation and peripheral thermal damage is a concern when using high pulse repetition rates. Peripheral thermal damage can adversely impact the mechanical strength of the irradiated tissue, particularly for dentin, and reduce the adhesion characteristics of the modified surfaces. The interpulpal temperature rise was recorded using microthermocouples situated at the roof of the pulp chamber on teeth that were occlusally ablated using a rapidly-scanned CO2 laser operating at 9.3 μm with a pulse duration of 10 to 15 μs and repetition rate of 300 Hz over a 2 min time course. The adhesion strength of laser treated enamel and dentin surfaces was measured for various laser scanning parameters with and without post-ablation acid etching using the single-plane shear test. The mechanical strength of laser-ablated dentin surfaces were determined via the four-point bend test and compared to control samples prepared with 320 grit wet sand paper to simulate conventional preparations. Thermocouple measurements indicated that the temperature remained below ambient temperature if water-cooling was used. There was no discoloration of either dentin or enamel laser treated surfaces, the surfaces were uniformly ablated, and there were no cracks visible. Four-point bend tests yielded mean mechanical strengths of 18.2 N (s.d. = 4.6) for ablated dentin and 18.1 N (s.d. = 2.7) for control (p > 0.05). Shear tests yielded mean bond strengths approaching 30 MPa for both enamel and dentin under certain irradiation conditions. These values were slightly lower than nonirradiated acid-etched control samples. Additional studies are needed to determine if the slightly lower bond strength than the acid-etched control samples is clinically significant. These measurements demonstrate that enamel and dentin surfaces can be rapidly ablated by CO2 lasers with minimal

  16. High-speed scanning ablation of dental hard tissues with a λ = 9.3 μm CO2 laser: adhesion, mechanical strength, heat accumulation, and peripheral thermal damage

    NASA Astrophysics Data System (ADS)

    Nguyen, Daniel; Chang, Kwang; Hedayatollahnajafi, Saba; Staninec, Michal; Chan, Kenneth; Lee, Robert; Fried, Daniel

    2011-07-01

    CO2 lasers can be operated at high laser pulse repetition rates for the rapid and precise removal of dental decay. Excessive heat accumulation and peripheral thermal damage is a concern when using high pulse repetition rates. Peripheral thermal damage can adversely impact the mechanical strength of the irradiated tissue, particularly for dentin, and reduce the adhesion characteristics of the modified surfaces. The interpulpal temperature rise was recorded using microthermocouples situated at the roof of the pulp chamber on teeth that were occlusally ablated using a rapidly-scanned CO2 laser operating at 9.3 μm with a pulse duration of 10 to 15 μs and repetition rate of 300 Hz over a 2 min time course. The adhesion strength of laser treated enamel and dentin surfaces was measured for various laser scanning parameters with and without post-ablation acid etching using the single-plane shear test. The mechanical strength of laser-ablated dentin surfaces were determined via the four-point bend test and compared to control samples prepared with 320 grit wet sand paper to simulate conventional preparations. Thermocouple measurements indicated that the temperature remained below ambient temperature if water-cooling was used. There was no discoloration of either dentin or enamel laser treated surfaces, the surfaces were uniformly ablated, and there were no cracks visible. Four-point bend tests yielded mean mechanical strengths of 18.2 N (s.d. = 4.6) for ablated dentin and 18.1 N (s.d. = 2.7) for control (p > 0.05). Shear tests yielded mean bond strengths approaching 30 MPa for both enamel and dentin under certain irradiation conditions. These values were slightly lower than nonirradiated acid-etched control samples. Additional studies are needed to determine if the slightly lower bond strength than the acid-etched control samples is clinically significant. These measurements demonstrate that enamel and dentin surfaces can be rapidly ablated by CO2 lasers with minimal

  17. Creep contributes to the fatigue behavior of bovine trabecular bone.

    PubMed

    Bowman, S M; Guo, X E; Cheng, D W; Keaveny, T M; Gibson, L J; Hayes, W C; McMahon, T A

    1998-10-01

    Repetitive, low-intensity loading from normal daily activities can generate fatigue damage in trabecular bone, a potential cause of spontaneous fractures of the hip and spine. Finite element models of trabecular bone (Guo et al., 1994) suggest that both creep and slow crack growth contribute to fatigue failure. In an effort to characterize these damage mechanisms experimentally, we conducted fatigue and creep tests on 85 waisted specimens of trabecular bone obtained from 76 bovine proximal tibiae. All applied stresses were normalized by the previously measured specimen modulus. Fatigue tests were conducted at room temperature; creep tests were conducted at 4, 15, 25, 37, 45, and 53 degrees C in a custom-designed apparatus. The fatigue behavior was characterized by decreasing modulus and increasing hysteresis prior to failure. The hysteresis loops progressively displaced along the strain axis, indicating that creep was also involved in the fatigue process. The creep behavior was characterized by the three classical stages of decreasing, constant, and increasing creep rates. Strong and highly significant power-law relationships were found between cycles-to-failure, time-to-failure, steady-state creep rate, and the applied loads. Creep analyses of the fatigue hysteresis loops also generated strong and highly significant power law relationships for time-to-failure and steady-state creep rate. Lastly, the products of creep rate and time-to-failure were constant for both the fatigue and creep tests and were equal to the measured failure strains, suggesting that creep plays a fundamental role in the fatigue behavior of trabecular bone. Additional analysis of the fatigue strain data suggests that creep and slow crack growth are not separate processes that dominate at high and low loads, respectively, but are present throughout all stages of fatigue.

  18. Fatigue performance and cyclic softening of F82H, a ferritic martensic steel

    SciTech Connect

    Stubbins, J.F.; Gelles, D.S.

    1996-04-01

    The room temperature fatigue performance of F82H has been examined. The fatigue life was determined in a series of strain-controlled tests where the stress level was monitored as a function of the number of accrued cycles. Fatigue lives in the range of 10{sup 3} to 10{sup 6} cycles to failure were examined. The fatigue performance was found to be controlled primarily by the elastic strain range over most of the range of fatigue lives examined. Only at low fatigue lives did the plastic strain range contribute to the response. However, when the significant plastic strain did contribute, the material showed a tendency to cyclically soften. That is the load carrying capability of the material degrades with accumulated fatigue cycles. The overall fatigue performance of the F82H alloy was found to be similiar to other advanced martensitic steels, but lower than more common low alloy steels which possess lower yield strengths.

  19. The Effect of Osteoporosis Treatments on Fatigue Properties of Cortical Bone Tissue

    PubMed Central

    Brock, Garry R.; Chen, Julia T.; Ingraffea, Anthony R.; MacLeay, Jennifer; Pluhar, G. Elizabeth; Boskey, Adele L.; van der Meulen, Marjolein C.H.

    2015-01-01

    Bisphosphonates are commonly prescribed for treatment of osteoporosis. Long-term use of bisphosphonates has been correlated to atypical femoral fractures (AFF). AFFs arise from fatigue damage to bone tissue that cannot be repaired due to pharmacologic treatments. Despite fatigue being the primary damage mechanism of AFFs, the effects of osteoporosis treatments on fatigue properties of cortical bone are unknown. To examine if fatigue-life differences occur in bone tissue after different pharmacologic treatments for osteoporosis, we tested bone tissue from the femurs of sheep given a metabolic acidosis diet to induce osteoporosis, followed by treatment with a selective estrogen reception modulator (raloxifene), a bisphosphonate (alendronate or zoledronate), or parathyroid hormone (teriparatide, PTH). Beams of cortical bone tissue were created and tested in four-point bending fatigue to failure. Tissues treated with alendronate had reduced fatigue life and less modulus loss at failure compared to other treatments, while tissue treated with PTH had a prolonged fatigue life. No loss of fatigue life occurred with zoledronate treatment despite its greater binding affinity and potency compared to alendronate. Tissue mineralization measured by microCT did not explain the differences seen in fatigue behavior. Increased fatigue life with PTH suggests that current treatment methods for AFF could have beneficial effects for restoring fatigue life. These results indicate that fatigue life differs with each type of osteoporosis treatment. PMID:25642445

  20. A status report on chronic fatigue syndrome.

    PubMed Central

    Natelson, Benjamin H; Lange, Gudrun

    2002-01-01

    Medical history has shown that clinical disease entities or syndromes are composed of many subgroups--each with its own cause and pathogenesis. Although we cannot be sure, we expect the same outcome for chronic fatigue syndrome (CFS), a medically unexplained condition characterized by disabling fatigue accompanied by infectious, rheumatological, and neuropsychiatric symptoms. Although the ailment clearly can occur after severe infection, no convincing data exist to support an infectious (or immunologic) process in disease maintenance. Instead, data point to several possible pathophysiological processes: a covert encephalopathy, impaired physiological capability to respond to physical and mental stressors, and psychological factors related to concerns about effort exacerbating symptoms. Each of these is under intense investigation. In addition, some data do exist to indicate that environmental agents also can elicit a state of chronic fatigue. We expect data to accumulate to support the belief that CFS has multiple causes. PMID:12194905