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Sample records for accumulated higher amounts

  1. Characters related to higher starch accumulation in cassava storage roots

    PubMed Central

    Li, You-Zhi; Zhao, Jian-Yu; Wu, San-Min; Fan, Xian-Wei; Luo, Xing-Lu; Chen, Bao-Shan

    2016-01-01

    Cassava (Manihot esculenta) is valued mainly for high content starch in its roots. Our understanding of mechanisms promoting high starch accumulation in the roots is, however, still very limited. Two field-grown cassava cultivars, Huanan 124(H124) with low root starch and Fuxuan 01(F01) with high root starch, were characterised comparatively at four main growth stages. Changes in key sugars in the leaves, stems and roots seemed not to be strongly associated with the final amount of starch accumulated in the roots. However, when compared with H124, F01 exhibited a more compact arrangement of xylem vascular bundles in the leaf axils, much less callose around the phloem sieve plates in the stems, higher starch synthesis-related enzymatic activity but lower amylase activity in the roots, more significantly up-regulated expression of related genes, and a much higher stem flow rate (SFR). In conclusion, higher starch accumulation in the roots results from the concurrent effects of powerful stem transport capacity highlighted by higher SFR, high starch synthesis but low starch degradation in the roots, and high expression of sugar transporter genes in the stems. A model of high starch accumulation in cassava roots was therefore proposed and discussed. PMID:26892156

  2. Characters related to higher starch accumulation in cassava storage roots.

    PubMed

    Li, You-Zhi; Zhao, Jian-Yu; Wu, San-Min; Fan, Xian-Wei; Luo, Xing-Lu; Chen, Bao-Shan

    2016-01-01

    Cassava (Manihot esculenta) is valued mainly for high content starch in its roots. Our understanding of mechanisms promoting high starch accumulation in the roots is, however, still very limited. Two field-grown cassava cultivars, Huanan 124(H124) with low root starch and Fuxuan 01(F01) with high root starch, were characterised comparatively at four main growth stages. Changes in key sugars in the leaves, stems and roots seemed not to be strongly associated with the final amount of starch accumulated in the roots. However, when compared with H124, F01 exhibited a more compact arrangement of xylem vascular bundles in the leaf axils, much less callose around the phloem sieve plates in the stems, higher starch synthesis-related enzymatic activity but lower amylase activity in the roots, more significantly up-regulated expression of related genes, and a much higher stem flow rate (SFR). In conclusion, higher starch accumulation in the roots results from the concurrent effects of powerful stem transport capacity highlighted by higher SFR, high starch synthesis but low starch degradation in the roots, and high expression of sugar transporter genes in the stems. A model of high starch accumulation in cassava roots was therefore proposed and discussed. PMID:26892156

  3. How and why does tomato accumulate a large amount of GABA in the fruit?

    PubMed Central

    Takayama, Mariko; Ezura, Hiroshi

    2015-01-01

    Gamma-aminobutyric acid (GABA) has received much attention as a health-promoting functional compound, and several GABA-enriched foods have been commercialized. In higher plants, GABA is primarily metabolized via a short pathway called the GABA shunt. The GABA shunt bypasses two steps (the oxidation of α-ketoglutarate to succinate) of the tricarboxylic acid (TCA) cycle via reactions catalyzed by three enzymes: glutamate decarboxylase, GABA transaminase, and succinic semialdehyde dehydrogenase. The GABA shunt plays a major role in primary carbon and nitrogen metabolism and is an integral part of the TCA cycle under stress and non-stress conditions. Tomato is one of the major crops that accumulate a relatively high level of GABA in its fruits. The GABA levels in tomato fruits dramatically change during fruit development; the GABA levels increase from flowering to the mature green stage and then rapidly decrease during the ripening stage. Although GABA constitutes up to 50% of the free amino acids at the mature green stage, the molecular mechanism of GABA accumulation and the physiological function of GABA during tomato fruit development remain unclear. In this review, we summarize recent studies of GABA accumulation in tomato fruits and discuss the potential biological roles of GABA in tomato fruit development. PMID:26322056

  4. Dual Mode NOx Sensor: Measuring Both the Accumulated Amount and Instantaneous Level at Low Concentrations

    PubMed Central

    Groß, Andrea; Beulertz, Gregor; Marr, Isabella; Kubinski, David J.; Visser, Jaco H.; Moos, Ralf

    2012-01-01

    The accumulating-type (or integrating-type) NOx sensor principle offers two operation modes to measure low levels of NOx: The direct signal gives the total amount dosed over a time interval and its derivative the instantaneous concentration. With a linear sensor response, no baseline drift, and both response times and recovery times in the range of the gas exchange time of the test bench (5 to 7 s), the integrating sensor is well suited to reliably detect low levels of NOx. Experimental results are presented demonstrating the sensor’s integrating properties for the total amount detection and its sensitivity to both NO and to NO2. We also show the correlation between the derivative of the sensor signal and the known gas concentration. The long-term detection of NOx in the sub-ppm range (e.g., for air quality measurements) is discussed. Additionally, a self-adaption of the measurement range taking advantage of the temperature dependency of the sensitivity is addressed. PMID:22736980

  5. Higher transcription levels in ascorbic acid biosynthetic and recycling genes were associated with higher ascorbic acid accumulation in blueberry.

    PubMed

    Liu, Fenghong; Wang, Lei; Gu, Liang; Zhao, Wei; Su, Hongyan; Cheng, Xianhao

    2015-12-01

    In our preliminary study, the ripe fruits of two highbush blueberry (Vaccinium corymbosum L.) cultivars, cv 'Berkeley' and cv 'Bluecrop', were found to contain different levels of ascorbic acid. However, factors responsible for these differences are still unknown. In the present study, ascorbic acid content in fruits was compared with expression profiles of ascorbic acid biosynthetic and recycling genes between 'Bluecrop' and 'Berkeley' cultivars. The results indicated that the l-galactose pathway was the predominant route of ascorbic acid biosynthesis in blueberry fruits. Moreover, higher expression levels of the ascorbic acid biosynthetic genes GME, GGP, and GLDH, as well as the recycling genes MDHAR and DHAR, were associated with higher ascorbic acid content in 'Bluecrop' compared with 'Berkeley', which indicated that a higher efficiency ascorbic acid biosynthesis and regeneration was likely to be responsible for the higher ascorbic acid accumulation in 'Bluecrop'. PMID:26041210

  6. Accumulating Human Capital While Increasing Educational Inequality: A Study on Higher Education Policy in China

    ERIC Educational Resources Information Center

    Chan, Wing Kit; Ngok, Kinglun

    2011-01-01

    Since 1999, the expansion of higher education has been viewed as an important step in accumulating human capital for China that was to gradually open its domestic sectors to the global market at a turbulent time at the turn of the century. Recent studies suggest that the improvement of human capital has succeeded in preparing China with a solid…

  7. Concomitant extracellular accumulation of alpha-keto acids and higher alcohols by Zygosaccharomyces rouxii.

    PubMed

    Van Der Sluis, Catrinus; Rahardjo, Yovita S P; Smit, Bart A; Kroon, Pieter J; Hartmans, Sybe; Ter Schure, Eelko G; Tramper, Johannes; Wijffels, Renéh

    2002-01-01

    Alpha-keto acids are key intermediates in the formation of higher alcohols, important flavor components in soy sauce, and produced by the salt-tolerant yeast Zygosaccharomyces rouxii. Unlike most of the higher alcohols, the alpha-keto acids are usually not extracellularly accumulated by Z. rouxii when it is cultivated with ammonium as the sole nitrogen source. To facilitate extracellular accumulation of the alpha-keto acids from aspartate-derived amino acid metabolism, the amino acids valine, leucine, threonine and methionine were exogenously supplied during batch and A-star cultivations of (routants of) Z. rouxii. It was shown that all alpha-keto acids from the aspartate-derived amino acid metabolism, except alpha-ketobutyrate, could be extracellularly accumulated. In addition, it appeared from the concomitant extracellular accumulation of alpha-keto acids and higher alcohols that in Z. rouxii, valine, leucine and methionine were converted via Ehrlich pathways similar to those in Saccharomyces cerevisiae. Unlike these amino acids, threonine was converted via both the Ehrlich and amino acid biosynthetic pathways in Z. rouxii. PMID:16233175

  8. Energy expended and knee joint load accumulated when walking, running, or standing for the same amount of time.

    PubMed

    Miller, Ross H; Edwards, W Brent; Deluzio, Kevin J

    2015-01-01

    Evidence suggests prolonged bouts of sitting are unhealthy, and some public health messages have recently recommended replacing sitting with more standing. However, the relative benefits of replacing sitting with standing compared to locomotion are not known. Specifically, the biomechanical consequences of standing compared to other sitting-alternatives like walking and running are not well known and are usually not considered in studies on sitting. We compared the total knee joint load accumulated (TKJLA) and the total energy expended (TEE) when performing either walking, running, or standing for a common exercise bout duration (30 min). Walking and running both (unsurprisingly) had much more TEE than standing (+300% and +1100%, respectively). TKJLA was similar between walking and standing and 74% greater in running. The results suggest that standing is a poor replacement for walking and running if one wishes to increases energy expenditure, and may be particularly questionable for use in individuals at-risk for knee osteoarthritis due to its surprisingly high TKJLA (just as high as walking, 56% of the load in running) and the type of loading (continuous compression) it places on cartilage. However, standing has health benefits as an "inactivity interrupter" that extend beyond its direct energy expenditure. We suggest that future studies on standing as an inactivity intervention consider the potential biomechanical consequences of standing more often throughout the day, particularly in the case of prolonged bouts of standing. PMID:25455208

  9. Boron uptake and accumulation by higher plants: A literature review: Final report

    SciTech Connect

    Sposito, G.; Calderone, S.J.

    1988-05-01

    This study provides a review of the literature on boron uptake and accumulation by higher plants, particularly trees. It addresses those aspects of the soil chemistry of boron that are most relevant to uptake by trees, then discusses the plant biochemistry of boron, its uptake and accumulation in plant tissue, and its phytotoxicity symptoms in plant species. The literature reviewed suggests that boron uptake is accomplished by a passive, massflow mechanism, as opposed to a metabolic process, with the most likely chemical form taken up being the neutral complex, H/sub 3/BO/sub 3/. The biochemical role of boron is not well understood, but evidence exists for its involvement with carbohydrate transformations and the control of growth-regulating compounds. Because of the mass-flow uptake mechanism, the distribution of boron in trees is connected intimately with the patterns of transpiration, which are species-dependent. Precise data on the effect of plant genotype on boron uptake, however, were not found in the published literature. The phytotoxicity symptoms of boron in trees also are species-dependent, although a consensus does exist as to the general nature of external symptoms and the basis of boron tolerance.

  10. Acidophilic green alga Pseudochlorella sp. YKT1 accumulates high amount of lipid droplets under a nitrogen-depleted condition at a low-pH.

    PubMed

    Hirooka, Shunsuke; Higuchi, Sumio; Uzuka, Akihiro; Nozaki, Hisayoshi; Miyagishima, Shin-ya

    2014-01-01

    Microalgal storage lipids are considered to be a promising source for next-generation biofuel feedstock. However, microalgal biodiesel is not yet economically feasible due to the high cost of production. One of the reasons for this is that the use of a low-cost open pond system is currently limited because of the unavoidable contamination with undesirable organisms. Extremophiles have an advantage in culturing in an open pond system because they grow in extreme environments toxic to other organisms. In this study, we isolated the acidophilic green alga Pseudochlorella sp. YKT1 from sulfuric acid mine drainage in Nagano Prefecture, Japan. The vegetative cells of YKT1 display the morphological characteristics of Trebouxiophyceae and molecular phylogenetic analyses indicated it to be most closely related to Pseudochlorella pringsheimii. The optimal pH and temperature for the growth of YKT1 are pH 3.0-5.0 and a temperature 20-25°C, respectively. Further, YKT1 is able to grow at pH 2.0 and at 32°C, which corresponds to the usual water temperature in the outdoors in summer in many countries. YKT1 accumulates a large amount of storage lipids (∼30% of dry weigh) under a nitrogen-depleted condition at low-pH (pH 3.0). These results show that acidophilic green algae will be useful for industrial applications by acidic open culture systems. PMID:25221913

  11. Accumulation of Polyhydroxyalkanoic Acid Containing Large Amounts of Unsaturated Monomers in Pseudomonas fluorescens BM07 Utilizing Saccharides and Its Inhibition by 2-Bromooctanoic Acid

    PubMed Central

    Lee, Ho-Joo; Choi, Mun Hwan; Kim, Tae-Un; Yoon, Sung Chul

    2001-01-01

    A psychrotrophic bacterium, Pseudomonas fluorescens BM07, which is able to accumulate polyhydroxyalkanoic acid (PHA) containing large amounts of 3-hydroxy-cis-5-dodecenoate unit up to 35 mol% in the cell from unrelated substrates such as fructose, succinate, etc., was isolated from an activated sludge in a municipal wastewater treatment plant. When it was grown on heptanoic acid (C7) to hexadecanoic acid (C16) as the sole carbon source, the monomer compositional characteristics of the synthesized PHA were similar to those observed in other fluorescent pseudomonads belonging to rRNA homology group I. However, growth on stearic acid (C18) led to no PHA accumulation, but instead free stearic acid was stored in the cell. The existence of the linkage between fatty acid de novo synthesis and PHA synthesis was confirmed by using inhibitors such as acrylic acid and two other compounds, 2-bromooctanoic acid and 4-pentenoic acid, which are known to inhibit β-oxidation enzymes in animal cells. Acrylic acid completely inhibited PHA synthesis at a concentration of 4 mM in 40 mM octanoate-grown cells, but no inhibition of PHA synthesis occurred in 70 mM fructose-grown cells in the presence of 1 to 5 mM acrylic acid. 2-Bromooctanoic acid and 4-pentenoic acid were found to much inhibit PHA synthesis much more strongly in fructose-grown cells than in octanoate-grown cells over concentrations ranging from 1 to 5 mM. However, 2-bromooctanoic acid and 4-pentenoic acid did not inhibit cell growth at all in the fructose media. Especially, with the cells grown on fructose, 2-bromooctanoic acid exhibited a steep rise in the percent PHA synthesis inhibition over a small range of concentrations below 100 μM, a finding indicative of a very specific inhibition, whereas 4-pentenoic acid showed a broad, featureless concentration dependence, suggesting a rather nonspecific inhibition. The apparent inhibition constant Ki (the concentration for 50% inhibition of PHA synthesis) for 2

  12. Selection and Reduced Population Size Cannot Explain Higher Amounts of Neandertal Ancestry in East Asian than in European Human Populations

    PubMed Central

    Kim, Bernard Y.; Lohmueller, Kirk E.

    2015-01-01

    It has been hypothesized that the greater proportion of Neandertal ancestry in East Asians than in Europeans is due to the fact that purifying selection is less effective at removing weakly deleterious Neandertal alleles from East Asian populations. Using simulations of a broad range of models of selection and demography, we have shown that this hypothesis cannot account for the higher proportion of Neandertal ancestry in East Asians than in Europeans. Instead, more complex demographic scenarios, most likely involving multiple pulses of Neandertal admixture, are required to explain the data. PMID:25683122

  13. Sheep primary astrocytes under starvation conditions express higher amount of LC3 II autophagy marker than neurons.

    PubMed

    Mura, Emilio; Lepore, Gianluca; Zedda, Marco; Giua, Stefano; Farina, Vittorio

    2014-03-01

    Autophagy is a general term for the degradation of cytoplasmic components within lysosomes. Recent studies have clearly demonstrated that autophagy has a greater variety of physiological and pathophysiological roles than expected, such as starvation adaptation, intracellular protein and organelle clearance, development, anti-aging, elimination of microorganisms, cell death, tumor suppression and antigen presentation. MAP-LC3 is one of the most common markers  to evaluate autophagic processes. In our study, the autophagic activity in neurons and astrocytes from sheep brain under starving conditions was evaluated. In order to detect LC3 immunoreactivity, confocal analysis by double immunofluorescence was performed together with the cell type markers: GFAP to identify astrocytes, β-III tubulin to identify neurons. The results show that astrocytes are characterized by LC3-positive areas, which increase in a time-dependent manner. In contrast, LC3 immunoreactivity was very weak in neurons. Therefore, it can be assumed that astrocytes show a higher capability than neurons to cope with stress and exhibit a stronger autophagic response. PMID:25181596

  14. Conditional Health-Related Benefits of Higher Education: An Assessment of Compensatory versus Accumulative Mechanisms

    PubMed Central

    Bauldry, Shawn

    2014-01-01

    A college degree is associated with a range of health-related benefits, but the effects of higher education are known to vary across different population subgroups. Competing theories have been proposed for whether people from more or less advantaged backgrounds or circumstances will gain greater health-related benefits from a college degree. This study draws on data from the National Longitudinal Study of Adolescent Health (Add Health) and recently developed models for analyzing heterogeneous treatment effects to examine how the effect of obtaining a college degree on the self-rated health of young adults varies across the likelihood of obtaining a college degree, a summary measure of advantage/disadvantage. Results indicate that a college degree has a greater effect on self-rated health for people from advantaged backgrounds. This finding differs from two recent studies, and possible reasons for the contrasting findings are discussed. PMID:24768780

  15. Exploring Effective Strategies for Increasing the Amount of Moderate-to-Vigorous Physical Activity Children Accumulate during Recess: A Quasi-Experimental Intervention Study

    ERIC Educational Resources Information Center

    Efrat, Merav W.

    2013-01-01

    Background: Less than half of elementary children meet the physical activity recommendations of 30 to 60 minutes of moderate-to-vigorous physical activity (MVPA) on a daily basis. Recess provides the single biggest opportunity for children to accumulate MVPA. This study explored whether a teacher's social prompting to be active during recess…

  16. Accumulation of planets into the proto-planetary cloud as a process of occurring an amount of characteristic scales into the nonlinear self organized dynamical systems

    NASA Astrophysics Data System (ADS)

    Professor Khachay, Yurie

    2015-04-01

    Two characteristic times are significant for evolution the interior of the homogeneous proto-planetary cloud: the time of bodies free fall towards the clouds mass center and the time of sound distribution through the cloud. With the beginning of proto-planetary disk fragmentation and accumulation of the proto-planets from the bodies and particles there are formed matter content heterogeneities of the finite dimension, heterogeneities of temperature, density and values of kinetic coefficients. The system became more and more complicated with interior interconnections. By the growing of the bodies the difference between the values of the characteristic times and dimensions become larger. The dynamical evolution of the system we could observe with use the numerical modeling of the Earth and Moon formation into the 3-D model [1,2]. The fact, that the linear dimensions of the objects during the accumulation process change from the centimeter and meter dimensions to some thousands of kilometers significantly prevent the mathematical description of these processes. The corresponding values of the no dimensional similarity criterions, which are included into the systems of differential equations, which describe the proto-planetary growing, the conditions for entropy and mass on the growing surface, the equations of the impulse balance, energy and mass into the interior parts of the planet change on an orders of values. Therefore we used very detailed space and time grids for solution the problem using the method of finite differences. The additional complications occur according to necessity to take into account the nonlinear dependence of matter viscosity from the temperature, pressure and chemical matter content. At last we took into account the principal random distribution of heterogeneities, stipulated by bodies and particles falling. Only progression towards that direction and constructing corresponding systems of observation and interpretation allow to hope

  17. The influence of biotic and abiotic factors on (137)Cs accumulation in higher fungi after the accident at Chernobyl NPP.

    PubMed

    Zarubina, N

    2016-09-01

    Levels of soil contamination with (137)Cs, the belonging of fungi to a certain ecological group, the localization depth of the main part of mycelium in soil are the primary factors influencing the value of (137)Cs specific activity in higher fungi after the accident at Chernobyl NPP. It has been found that the value of (137)Cs specific activity in fungi of one species could vary by more than 10 times during a vegetation period. A correlation between the changes of (137)Cs content in fungi during the vegetation period and the amount of precipitates during various periods preceding the collection of samples has not been determined. An assumption has been proposed stating dependence between peculiarities of mycelium growth during the vegetation period and the changes of (137)Cs specific activity in fungi. PMID:26690320

  18. Correlated Changes in the Activity, Amount of Protein, and Abundance of Transcript of NADPH:Protochlorophyllide Oxidoreductase and Chlorophyll Accumulation during Greening of Cucumber Cotyledons.

    PubMed Central

    Yoshida, K.; Chen, R. M.; Tanaka, A.; Teramoto, H.; Tanaka, R.; Timko, M. P.; Tsuji, H.

    1995-01-01

    Changes in the activity and abundance of NADPH:protochlorophyllide oxidoreductase (NPR) and the abundance of mRNA encoding it were examined during the greening of 5-d-old etiolated cucumber cotyledons under continuous illumination. To measure NPR activity in the extracts from fully greened tissues, we have developed an improved method of assay. Upon exposure of etiolated cotyledons to light, NPR activity decreased rapidly within the first 2 h of exposure. Thereafter, enzymatic activity increased transiently, reaching a submaximum level at 12 h, and decreased slowly. The level of immunodetectable NPR protein followed the same pattern of changes during 96 h of greening as observed for NPR activity. The NPR mRNA in etiolated cotyledons disappeared quickly in the 1st h of irradiation. However, the level of mRNA increased thereafter to reach 3-fold or more of the dark level at 12 h and then decreased. The changes in the activity, protein level, and mRNA level after the first rapid decreases corresponded chronologically and nearly paralleled the increase in the rate of chlorophyll accumulation. These findings suggest that the greening of cucumber cotyledons is regulated basically by the level of NPR protein without activation or repression of enzymatic activity and that NPR mRNA increased by light maintains the level of enzyme protein necessary for greening. PMID:12228591

  19. Complexation and Toxicity of Copper in Higher Plants. I. Characterization of Copper Accumulation, Speciation, and Toxicity in Crassula helmsii as a New Copper Accumulator1[W][OA

    PubMed Central

    Küpper, Hendrik; Götz, Birgit; Mijovilovich, Ana; Küpper, Frithjof C.; Meyer-Klaucke, Wolfram

    2009-01-01

    The amphibious water plant Crassula helmsii is an invasive copper (Cu)-tolerant neophyte in Europe. It now turned out to accumulate Cu up to more than 9,000 ppm in its shoots at 10 μm (=0.6 ppm) Cu2+ in the nutrient solution, indicating that it is a Cu hyperaccumulator. We investigated uptake, binding environment, and toxicity of Cu in this plant under emerged and submerged conditions. Extended x-ray absorption fine structure measurements on frozen-hydrated samples revealed that Cu was bound almost exclusively by oxygen ligands, likely organic acids, and not any sulfur ligands. Despite significant differences in photosynthesis biochemistry and biophysics between emerged and submerged plants, no differences in Cu ligands were found. While measurements of tissue pH confirmed the diurnal acid cycle typical for Crassulacean acid metabolism, Δ13C measurements showed values typical for regular C3 photosynthesis. Cu-induced inhibition of photosynthesis mainly affected the photosystem II (PSII) reaction center, but with some unusual features. Most obviously, the degree of light saturation of electron transport increased during Cu stress, while maximal dark-adapted PSII quantum yield did not change and light-adapted quantum yield of PSII photochemistry decreased particularly in the first 50 s after onset of actinic irradiance. This combination of changes, which were strongest in submerged cultures, shows a decreasing number of functional reaction centers relative to the antenna in a system with high antenna connectivity. Nonphotochemical quenching, in contrast, was modified by Cu mainly in emerged cultures. Pigment concentrations in stressed plants strongly decreased, but no changes in their ratios occurred, indicating that cells either survived intact or died and bleached quickly. PMID:19641032

  20. Accumulation of leginsulin, a hormone-like bioactive peptide, is drastically higher in Asian than in North American soybean accessions

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Leginsulin, a peptide made up of 37 amino acids, is homologous to pea albumin (PA1b) and belongs to the cysteine-knot family. Even though the physiological function and three-dimensional structure of leginsulin have been explored, little is known about their expression, accumulation and distribution...

  1. Accumulate-Repeat-Accumulate-Accumulate-Codes

    NASA Technical Reports Server (NTRS)

    Divsalar, Dariush; Dolinar, Sam; Thorpe, Jeremy

    2004-01-01

    Inspired by recently proposed Accumulate-Repeat-Accumulate (ARA) codes [15], in this paper we propose a channel coding scheme called Accumulate-Repeat-Accumulate-Accumulate (ARAA) codes. These codes can be seen as serial turbo-like codes or as a subclass of Low Density Parity Check (LDPC) codes, and they have a projected graph or protograph representation; this allows for a high-speed iterative decoder implementation using belief propagation. An ARAA code can be viewed as a precoded Repeat-and-Accumulate (RA) code with puncturing in concatenation with another accumulator, where simply an accumulator is chosen as the precoder; thus ARAA codes have a very fast encoder structure. Using density evolution on their associated protographs, we find examples of rate-lJ2 ARAA codes with maximum variable node degree 4 for which a minimum bit-SNR as low as 0.21 dB from the channel capacity limit can be achieved as the block size goes to infinity. Such a low threshold cannot be achieved by RA or Irregular RA (IRA) or unstructured irregular LDPC codes with the same constraint on the maximum variable node degree. Furthermore by puncturing the accumulators we can construct families of higher rate ARAA codes with thresholds that stay close to their respective channel capacity thresholds uniformly. Iterative decoding simulation results show comparable performance with the best-known LDPC codes but with very low error floor even at moderate block sizes.

  2. [Animal nutrition for veterinarians--case study: colic in ponies in a "petting zoo" caused by ingestion of higher amounts of wheat (Triticum aestivum L.)].

    PubMed

    Wolf, P; Kamphues, J

    2006-10-01

    Nutritional disorders in domestic or pet animals depend not seldom on special situations, for example when different influences coincide concerning keeping, housing and offering of feed or water. These lead suddenly to high-risk situations, that can cause sometimes the death of the animal. The following case report deals with two ponies, that were kept on a so-called "petting zoo" and showed repeatedly colic symptoms (always after a weekend). During surgery in the stomach light yellow balls were found, that can be explained by ingestion of 1.0 to 1.5 kg wheat. This cereals consist--depending on variety--gluten, that can cause the observed conglobates. Due to the forming of such balls that can lead to health disorders in form of colics, gastritis or ruptures of the stomach the feeding of high amounts of wheat should be avoided. Snacks rich in fiber or high amounts of roughage (straw, hay) that are fed before opening of the zoo could be a practical alternative. PMID:17078533

  3. Introduction of double amidoxime group by double post surface modification on poly(vinylbenzyl chloride) beads for higher amounts of organic dyes, As (V) and Cr (VI) removal.

    PubMed

    Ajmal, Muhammad; Demirci, Sahin; Uzun, Yusuf; Siddiq, Mohammad; Aktas, Nahit; Sahiner, Nurettin

    2016-05-15

    In this study, the synthesis of micron-sized poly(vinylbenzyl chloride) (p(VBC)) beads and subsequent conversion of the reactive chloromethyl groups to double amidoxime group containing moieties by post modification is reported. The prepared beads were characterized by SEM and FT-IR spectroscopy. The amidoximated p(VBC) beads were used as adsorbent for the removal of organic dyes, such as eosin y (EY) and methyl orange (MO), and heavy metals containing complex ions such as dichromate (Cr2O7(2-)) and arsenate (HAsO4(2)(-)) from aqueous media. The effect of the adsorbent dose on the percent removal, the effect of initial concentration of adsorbates on the adsorption rate and their amounts were also investigated. The Langmuir, Freundlich and Temkin adsorption isotherms were applied to the adsorption processes. The results indicated that the adsorption of both dichromate and arsenate ions obeyed the Langmuir adsorption model. Interestingly, it was found that the prepared beads were capable of removing significant amounts of arsenate and dichromate ions from tap and river (Sarıcay, Canakkale-Turkey) water. PMID:26930538

  4. Arbuscular mycorrhiza increase artemisinin accumulation in Artemisia annua by higher expression of key biosynthesis genes via enhanced jasmonic acid levels.

    PubMed

    Mandal, Shantanu; Upadhyay, Shivangi; Wajid, Saima; Ram, Mauji; Jain, Dharam Chand; Singh, Ved Pal; Abdin, Malik Zainul; Kapoor, Rupam

    2015-07-01

    It is becoming increasingly evident that the formation of arbuscular mycorrhiza (AM) enhances secondary metabolite production in shoots. Despite mounting evidence, relatively little is known about the underlying mechanisms. This study suggests that increase in artemisinin concentration in Artemisia annua colonized by Rhizophagus intraradices is due to altered trichome density as well as transcriptional patterns that are mediated via enhanced jasmonic acid (JA) levels. Mycorrhizal (M) plants had higher JA levels in leaf tissue that may be due to induction of an allene oxidase synthase gene (AOS), encoding one of the key enzymes for JA production. Non-mycorrhizal (NM) plants were exogenously supplied with a range of methyl jasmonic acid concentrations. When leaves of NM and M plants with similar levels of endogenous JA were compared, these matched closely in terms of shoot trichome density, artemisinin concentration, and transcript profile of artemisinin biosynthesis genes. Mycorrhization increased artemisinin levels by increasing glandular trichome density and transcriptional activation of artemisinin biosynthesis genes. Transcriptional analysis of some rate-limiting enzymes of mevalonate and methyl erythritol phosphate (MEP) pathways revealed that AM increases isoprenoids by induction of the MEP pathway. A decline in artemisinin concentration in shoots of NM and M plants treated with ibuprofen (an inhibitor of JA biosynthesis) further confirmed the implication of JA in the mechanism of artemisinin production. PMID:25366131

  5. Myotubes from Severely Obese Type 2 Diabetic Subjects Accumulate Less Lipids and Show Higher Lipolytic Rate than Myotubes from Severely Obese Non-Diabetic Subjects

    PubMed Central

    Bakke, Siril S.; Kase, Eili T.; Moro, Cedric; Stensrud, Camilla; Damlien, Lisbeth; Ludahl, Marianne O.; Sandbu, Rune; Solheim, Brita Marie; Rustan, Arild C.; Hjelmesæth, Jøran; Thoresen, G. Hege; Aas, Vigdis

    2015-01-01

    About 80% of patients with type 2 diabetes are classified as overweight. However, only about 1/3 of severely obese subjects have type 2 diabetes. This indicates that several severely obese individuals may possess certain characteristics that protect them against type 2 diabetes. We therefore hypothesized that this apparent paradox could be related to fundamental differences in skeletal muscle lipid handling. Energy metabolism and metabolic flexibility were examined in human myotubes derived from severely obese subjects without (BMI 44±7 kg/m2) and with type 2 diabetes (BMI 43±6 kg/m2). Lower insulin sensitivity was observed in myotubes from severely obese subjects with type 2 diabetes. Lipolysis rate was higher, and oleic acid accumulation, triacylglycerol content, and fatty acid adaptability were lower in myotubes from severely obese subjects with type 2 diabetes compared to severely obese non-diabetic subjects. There were no differences in lipid distribution and mRNA and protein expression of the lipases HSL and ATGL, the lipase cofactor CGI-58, or the lipid droplet proteins PLIN2 and PLIN3. Glucose and oleic acid oxidation were also similar in cells from the two groups. In conclusion, myotubes established from severely obese donors with established type 2 diabetes had lower ability for lipid accumulation and higher lipolysis rate than myotubes from severely obese donors without diabetes. This indicates that a difference in intramyocellular lipid turnover might be fundamental in evolving type 2 diabetes. PMID:25790476

  6. A higher proportion of iron-rich leafy vegetables in a typical Burkinabe maize meal does not increase the amount of iron absorbed in young women.

    PubMed

    Cercamondi, Colin I; Icard-Vernière, Christèle; Egli, Ines M; Vernay, Marlène; Hama, Fatoumata; Brouwer, Inge D; Zeder, Christophe; Berger, Jacques; Hurrell, Richard F; Mouquet-Rivier, Claire

    2014-09-01

    Food-to-food fortification can be a promising approach to improve the low dietary iron intake and bioavailability from monotonous diets based on a small number of staple plant foods. In Burkina Faso, the common diet consists of a thick, cereal-based paste consumed with sauces composed of mainly green leaves, such as amaranth and jute leaves. Increasing the quantity of leaves in the sauces substantially increases their iron concentration. To evaluate whether increasing the quantity of leaves in sauces would provide additional bioavailable iron, an iron absorption study in 18 young women was conducted in Zurich, Switzerland. Burkinabe composite test meals consisting of the maize paste tô accompanied by an iron-improved amaranth sauce, an iron-improved jute sauce, or a traditional amaranth sauce were provided as multiple meals twice a day for 2 consecutive days. Iron absorption was measured as erythrocyte incorporation of stable iron isotopes. Mean fractional iron absorption from maize paste consumed with an iron-improved amaranth sauce (4.9%) did not differ from the same meal consumed with an iron-improved jute sauce (4.9%; P = 0.9), resulting in a similar quantity of total iron absorbed (679 vs. 578 μg; P = 0.3). Mean fractional iron absorption from maize paste accompanied by a traditional amaranth sauce (7.4%) was significantly higher than that from the other 2 meal types (P < 0.05), but the quantity of total iron absorbed was similar (591 μg; P = 0.4 and 0.7, respectively). A food-to-food fortification approach based on an increase in leafy vegetables does not provide additional bioavailable iron, presumably due to the high phenolic compound concentration of the leaves tested. Alternative measures, such as adding iron absorption enhancers to the sauces, need to be investigated to improve iron nutrition from Burkinabe maize meals. PMID:25031328

  7. Accumulation of DOC in Low Phosphate Low Chlorophyll (LPLC) area: is it related to higher production under high N:P ratio?

    NASA Astrophysics Data System (ADS)

    Mauriac, R.; Moutin, T.; Baklouti, M.

    2010-09-01

    The biogeochemistry of carbon and nutrients (N, P) in the surface layer of the ocean strongly depends on the interaction between C, N and P at the cell level and at the population level where interaction between primary producers (phytoplankton) and remineralizers (heterotrophic bacteria) impact the overall stock and dynamics of organic carbon. To understand these interactions in the surface layer of the Mediterranean Sea, we implemented, using Eco3M, a multi-element, steady state, mechanistic model. This cell-based model intend to represent the growth of phytoplankton and heterotrophic bacteria under various amount of nutrients. As a results, it displays the expected biogeochemical characteristics of the system and give us insight on the expected interaction between phytoplankton and heterotrophic bacteria both in term of competition for inorganic nutrients and in term of commensalism for organic carbon. In this study, we found a good quantitative agreement between model results and literrature data for stocks and fluxes of the western Mediterranean basin. In addition, for phytoplankton we show how the uncoupling between carbon production and growth could impact the overall DOC dynamic and based on these results, we proposed a new explanantion for the observed DOC accumulation in the surface layer of the Mediterranean Sea.

  8. Accumulate repeat accumulate codes

    NASA Technical Reports Server (NTRS)

    Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

    2004-01-01

    In this paper we propose an innovative channel coding scheme called 'Accumulate Repeat Accumulate codes' (ARA). This class of codes can be viewed as serial turbo-like codes, or as a subclass of Low Density Parity Check (LDPC) codes, thus belief propagation can be used for iterative decoding of ARA codes on a graph. The structure of encoder for this class can be viewed as precoded Repeat Accumulate (RA) code or as precoded Irregular Repeat Accumulate (IRA) code, where simply an accumulator is chosen as a precoder. Thus ARA codes have simple, and very fast encoder structure when they representing LDPC codes. Based on density evolution for LDPC codes through some examples for ARA codes, we show that for maximum variable node degree 5 a minimum bit SNR as low as 0.08 dB from channel capacity for rate 1/2 can be achieved as the block size goes to infinity. Thus based on fixed low maximum variable node degree, its threshold outperforms not only the RA and IRA codes but also the best known LDPC codes with the dame maximum node degree. Furthermore by puncturing the accumulators any desired high rate codes close to code rate 1 can be obtained with thresholds that stay close to the channel capacity thresholds uniformly. Iterative decoding simulation results are provided. The ARA codes also have projected graph or protograph representation that allows for high speed decoder implementation.

  9. Accumulate-Repeat-Accumulate-Accumulate Codes

    NASA Technical Reports Server (NTRS)

    Divsalar, Dariush; Dolinar, Samuel; Thorpe, Jeremy

    2007-01-01

    Accumulate-repeat-accumulate-accumulate (ARAA) codes have been proposed, inspired by the recently proposed accumulate-repeat-accumulate (ARA) codes. These are error-correcting codes suitable for use in a variety of wireless data-communication systems that include noisy channels. ARAA codes can be regarded as serial turbolike codes or as a subclass of low-density parity-check (LDPC) codes, and, like ARA codes they have projected graph or protograph representations; these characteristics make it possible to design high-speed iterative decoders that utilize belief-propagation algorithms. The objective in proposing ARAA codes as a subclass of ARA codes was to enhance the error-floor performance of ARA codes while maintaining simple encoding structures and low maximum variable node degree.

  10. The Indefinite Accumulation of Finite Amounts: A Socratic Educative Experience

    ERIC Educational Resources Information Center

    Navarro, Maria Angeles; Carreras, Pedro Perez

    2011-01-01

    We present a semi-structured clinic interview designed to ease the mental construction of a suitable concept-image of the notion of convergence for series of positive numbers. Cognitive obstacles will manifest themselves along the interview and we shall deal with them and teach the student how to overcome them. A special computer generated tool…

  11. Astrocytic and neuronal accumulation of elevated extracellular K+ with a 2/3 K+/Na+ flux ratio—consequences for energy metabolism, osmolarity and higher brain function

    PubMed Central

    Hertz, Leif; Xu, Junnan; Song, Dan; Yan, Enzhi; Gu, Li; Peng, Liang

    2013-01-01

    Brain excitation increases neuronal Na+ concentration by 2 major mechanisms: (i) Na+ influx caused by glutamatergic synaptic activity; and (ii) action-potential-mediated depolarization by Na+ influx followed by repolarizating K+ efflux, increasing extracellular K+ concentration. This review deals mainly with the latter and it concludes that clearance of extracellular K+ is initially mainly effectuated by Na+,K+-ATPase-mediated K+ uptake into astrocytes, at K+ concentrations above ~10 mM aided by uptake of Na+,K+ and 2 Cl− by the cotransporter NKCC1. Since operation of the astrocytic Na+,K+-ATPase requires K+-dependent glycogenolysis for stimulation of the intracellular ATPase site, it ceases after normalization of extracellular K+ concentration. This allows K+ release via the inward rectifying K+ channel Kir4.1, perhaps after trans-astrocytic connexin- and/or pannexin-mediated K+ transfer, which would be a key candidate for determination by synchronization-based computational analysis and may have signaling effects. Spatially dispersed K+ release would have little effect on extracellular K+ concentration and allow K+ accumulation by the less powerful neuronal Na+,K+-ATPase, which is not stimulated by increases in extracellular K+. Since the Na+,K+-ATPase exchanges 3 Na+ with 2 K+, it creates extracellular hypertonicity and cell shrinkage. Hypertonicity stimulates NKCC1, which, aided by β-adrenergic stimulation of the Na+,K+-ATPase, causes regulatory volume increase, furosemide-inhibited undershoot in [K+]e and perhaps facilitation of the termination of slow neuronal hyperpolarization (sAHP), with behavioral consequences. The ion transport processes involved minimize ionic disequilibria caused by the asymmetric Na+,K+-ATPase fluxes. PMID:23986689

  12. Higher growth temperatures decreased net carbon assimilation and biomass accumulation of northern red oak seedlings near the southern limit of the species range.

    PubMed

    Wertin, Timothy M; McGuire, Mary Anne; Teskey, Robert O

    2011-12-01

    If an increase in temperature will limit the growth of a species, it will be in the warmest portion of the species distribution. Therefore, in this study we examined the effects of elevated temperature on net carbon assimilation and biomass production of northern red oak (Quercus rubra L.) seedlings grown near the southern limit of the species distribution. Seedlings were grown in chambers in elevated CO(2) (700 µmol mol(-1)) at three temperature conditions, ambient (tracking diurnal and seasonal variation in outdoor temperature), ambient +3 °C and ambient +6 °C, which produced mean growing season temperatures of 23, 26 and 29 °C, respectively. A group of seedlings was also grown in ambient [CO(2)] and ambient temperature as a check of the growth response to elevated [CO(2)]. Net photosynthesis and leaf respiration, photosynthetic capacity (V(cmax), J(max) and triose phosphate utilization (TPU)) and chlorophyll fluorescence, as well as seedling height, diameter and biomass, were measured during one growing season. Higher growth temperatures reduced net photosynthesis, increased respiration and reduced height, diameter and biomass production. Maximum net photosynthesis at saturating [CO(2)] and maximum rate of electron transport (J(max)) were lowest throughout the growing season in seedlings grown in the highest temperature regime. These parameters were also lower in June, but not in July or September, in seedlings grown at +3 °C above ambient, compared with those grown in ambient temperature, indicating no impairment of photosynthetic capacity with a moderate increase in air temperature. An unusual and potentially important observation was that foliar respiration did not acclimate to growth temperature, resulting in substantially higher leaf respiration at the higher growth temperatures. Lower net carbon assimilation was correlated with lower growth at higher temperatures. Total biomass at the end of the growing season decreased in direct proportion to the

  13. Fibres from flax overproducing β-1,3-glucanase show increased accumulation of pectin and phenolics and thus higher antioxidant capacity

    PubMed Central

    2013-01-01

    Background Recently, in order to improve the resistance of flax plants to pathogen infection, transgenic flax that overproduces β-1,3-glucanase was created. β-1,3-glucanase is a PR protein that hydrolyses the β-glucans, which are a major component of the cell wall in many groups of fungi. For this study, we used fourth-generation field-cultivated plants of the Fusarium -resistant transgenic line B14 to evaluate how overexpression of the β-1,3-glucanase gene influences the quantity, quality and composition of flax fibres, which are the main product obtained from flax straw. Results Overproduction of β-1,3-glucanase did not affect the quantity of the fibre obtained from the flax straw and did not significantly alter the essential mechanical characteristics of the retted fibres. However, changes in the contents of the major components of the cell wall (cellulose, hemicellulose, pectin and lignin) were revealed. Overexpression of the β-1,3-glucanase gene resulted in higher cellulose, hemicellulose and pectin contents and a lower lignin content in the fibres. Increases in the uronic acid content in particular fractions (with the exception of the 1 M KOH-soluble fraction of hemicelluloses) and changes in the sugar composition of the cell wall were detected in the fibres of the transgenic flax when compared to the contents for the control plants. The callose content was lower in the fibres of the transgenic flax. Additionally, the analysis of phenolic compound contents in five fractions of the cell wall revealed important changes, which were reflected in the antioxidant potential of these fractions. Conclusion Overexpression of the β-1,3-glucanase gene has a significant influence on the biochemical composition of flax fibres. The constitutive overproduction of β-1,3-glucanase causes a decrease in the callose content, and the resulting excess glucose serves as a substrate for the production of other polysaccharides. The monosaccharide excess redirects the phenolic

  14. Accumulation of intra-cellular polyphosphate in Chlorella vulgaris cells is related to indole-3-acetic acid produced by Azospirillum brasilense.

    PubMed

    Meza, Beatriz; de-Bashan, Luz E; Hernandez, Juan-Pablo; Bashan, Yoav

    2015-06-01

    Accumulation of intra-cellular phosphate, as polyphosphate, was measured when the microalga Chlorella vulgaris was immobilized in alginate with either of two wild-type strains of the microalgae growth-promoting bacterium Azospirillum brasilense or their corresponding IAA-attenuated mutants. Wild type strains of A. brasilense induced higher amounts of intra-cellular phosphate in Chlorella than their respective mutants. Calculations comparing intra-cellular phosphate accumulation by culture or net accumulation by the cell and the amount of IAA that was produced by each of these strains revealed that higher IAA was linked to higher accumulations of intra-cellular phosphate. Application of four levels of exogenous IAA reported for A. brasilense and their IAA-attenuated mutants to cultures of C. vulgaris enhanced accumulation of intra-cellular phosphate; the higher the content of IAA per culture or per single cell, the higher was the amount of accumulated phosphate. When an IAA-attenuated mutant was complemented with exogenous IAA, accumulation of intra-cellular phosphate at the culture level was even higher than phosphate accumulation with the respective wild type strains. When calculating the net accumulation of intra-cellular phosphate in the complementation experiment, net intra-cellular phosphate induced by the IAA-attenuated mutant was completely restored and was similar to the wild strains. We propose that IAA produced by A. brasilense is linked to polyphosphate accumulation in C. vulgaris. PMID:25797155

  15. Accumulate Repeat Accumulate Coded Modulation

    NASA Technical Reports Server (NTRS)

    Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

    2004-01-01

    In this paper we propose an innovative coded modulation scheme called 'Accumulate Repeat Accumulate Coded Modulation' (ARA coded modulation). This class of codes can be viewed as serial turbo-like codes, or as a subclass of Low Density Parity Check (LDPC) codes that are combined with high level modulation. Thus at the decoder belief propagation can be used for iterative decoding of ARA coded modulation on a graph, provided a demapper transforms the received in-phase and quadrature samples to reliability of the bits.

  16. 47 CFR 32.3100 - Accumulated depreciation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Accumulated depreciation. 32.3100 Section 32... Accumulated depreciation. (a) This account shall include the accumulated depreciation associated with the... with depreciation amounts concurrently charged to Account 6561, Depreciation...

  17. Arsenic accumulation by the aquatic fern Azolla: comparison of arsenate uptake, speciation and efflux by A. caroliniana and A. filiculoides.

    PubMed

    Zhang, Xin; Lin, Ai-Jun; Zhao, Fang-Jie; Xu, Guo-Zhong; Duan, Gui-Lan; Zhu, Yong-Guan

    2008-12-01

    This study investigates As accumulation and tolerance of the aquatic fern Azolla. Fifty strains of Azolla showed a large variation in As accumulation. The highest- and lowest-accumulating ferns among the 50 strains were chosen for further investigations. Azolla caroliniana accumulated two times more As than Azolla filiculoides owing to a higher influx velocity for arsenate. A. filiculoides was more resistant to external arsenate due to a lower uptake. Both strains showed a similar degree of tolerance to internal As. Arsenate and arsenite were the dominant As species in both Azolla strains, with methylated As species accounting for <5% of the total As. A. filiculoides had a higher proportion of arsenite than A. caroliniana. Both strains effluxed more arsenate than arsenite, and the amount of As efflux was proportional to the amount of As accumulation. The potential of growing Azolla in paddy fields to reduce As transfer from soil and water to rice should be further evaluated. PMID:18457908

  18. 26 CFR 1.666(a)-1A - Amount allocated.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 8 2013-04-01 2013-04-01 false Amount allocated. 1.666(a)-1A Section 1.666(a... Beginning Before January 1, 1969 § 1.666(a)-1A Amount allocated. (a) In general. In the case of a trust that... trusts that may accumulate income or that distribute corpus), section 666(a) prescribes rules...

  19. 26 CFR 1.666(a)-1A - Amount allocated.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 8 2012-04-01 2012-04-01 false Amount allocated. 1.666(a)-1A Section 1.666(a... Beginning Before January 1, 1969 § 1.666(a)-1A Amount allocated. (a) In general. In the case of a trust that... trusts that may accumulate income or that distribute corpus), section 666(a) prescribes rules...

  20. 26 CFR 1.666(a)-1A - Amount allocated.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 8 2014-04-01 2014-04-01 false Amount allocated. 1.666(a)-1A Section 1.666(a... Beginning Before January 1, 1969 § 1.666(a)-1A Amount allocated. (a) In general. In the case of a trust that... trusts that may accumulate income or that distribute corpus), section 666(a) prescribes rules...

  1. 26 CFR 1.666(a)-1A - Amount allocated.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Amount allocated. 1.666(a)-1A Section 1.666(a... Before January 1, 1969 § 1.666(a)-1A Amount allocated. (a) In general. In the case of a trust that is... that may accumulate income or that distribute corpus), section 666(a) prescribes rules for...

  2. 26 CFR 1.666(a)-1A - Amount allocated.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 8 2011-04-01 2011-04-01 false Amount allocated. 1.666(a)-1A Section 1.666(a... Beginning Before January 1, 1969 § 1.666(a)-1A Amount allocated. (a) In general. In the case of a trust that... trusts that may accumulate income or that distribute corpus), section 666(a) prescribes rules...

  3. Guidelines for Waste Accumulation Areas (WAAs)

    SciTech Connect

    Not Available

    1991-07-01

    The purpose of this document is to set conditions for establishing and maintaining areas for the accumulation of hazardous waste at LBL. Areas designed for accumulation of these wastes in quantities greater than 100 kg (220 lb) per month of solid waste or 55 gallons per month of liquid waste are called Waste Accumulation Areas (WAAs). Areas designed for accumulation of wastes in smaller amounts are called Satellite Accumulation Areas (SAAs). This document provides guidelines for employee and organizational responsibilities for WAAs; constructing a WAA; storing waste in a WAA; operating and maintaining a WAA, and responding to spills in a WAA. 4 figs.

  4. Effects of Weather, Time, and Pollution Level on the Amount of Particulate Matter Deposited on Leaves of Ligustrum lucidum

    PubMed Central

    Wang, Huixia; Shi, Hui; Wang, Yanhui

    2015-01-01

    This paper investigated the spatial and temporal variations in the amounts of PM accumulated on leaves of Ligustrum lucidum, a common evergreen tree species in North China. The effects of rainfall and wind on the amounts of PM deposited on foliage were also determined. The amounts of PM (g·m−2) retained by leaves of L. lucidum differed significantly among the sites (from 0.96 to 5.56) and over time (from 2.51 to 4.48). The largest amounts of PM on foliage of L. lucidum were observed on plants growing at the most polluted site. During the year, the highest and lowest accumulation of PM occurred in November and August, respectively. A considerable proportion of the accumulated PM on leaves was removed by rainfall events (28–48% of PM) and strong winds (27–36% of PM), and more precipitation or higher maximum wind speed could remove more PM from leaves. Rainfall removed mainly large and coarse particles, while fine particles adhered more strongly to the foliage. These results suggested that the effects of local weather conditions (e.g., rainfall, strong wind), different seasons, and pollution levels should be considered in evaluating total PM accumulation on leaves. PMID:25685849

  5. Copper accumulation by stickleback nests containing spiggin.

    PubMed

    Pinho, G L L; Martins, C M G; Barber, I

    2016-07-01

    The three-spined stickleback is a ubiquitous fish of marine, brackish and freshwater ecosystems across the Northern hemisphere that presents intermediate sensitivity to copper. Male sticklebacks display a range of elaborate reproductive behaviours that include nest construction. To build the nests, each male binds nesting material together using an endogenous glycoprotein nesting glue, known as 'spiggin'. Spiggin is a cysteine-rich protein and, therefore, potentially binds heavy metals present in the environment. The aim of this study was to investigate the capacity of stickleback nests to accumulate copper from environmental sources. Newly built nests, constructed by male fish from polyester threads in laboratory aquaria, were immersed in copper solutions ranging in concentration from 21.1-626.6 μg Cu L(-1). Bundles of polyester threads from aquaria without male fish were also immersed in the same copper solutions. After immersion, nests presented higher amounts of copper than the thread bundles, indicating a higher capacity of nests to bind this metal. A significant, positive correlation between the concentration of copper in the exposure solution and in the exposed nests was identified, but there was no such relationship for thread bundles. Since both spiggin synthesis and male courtship behaviour are under the control of circulating androgens, we predicted that males with high courtship scores would produce and secrete high levels of the spiggin protein. In the present study, nests built by high courtship score males accumulated more copper than those built by low courtship score males. Considering the potential of spiggin to bind metals, the positive relationship between fish courtship and spiggin secretion seems to explain the higher amount of copper on the nests from the fish showing high behaviour scores. Further work is now needed to determine the consequences of the copper binding potential of spiggin in stickleback nests for the health and survival of

  6. Dietary restriction, caloric value and the accumulation of hepatic fat

    PubMed Central

    2012-01-01

    Background Studies using laboratory animals under what are considered to be "standard" conditions normally offer unrestricted amounts of food to the animals, which can lead to metabolic disorders. Moreover, standard diets have different compositions. Aim Therefore, the aim of the present study was to assess the effects of two non-isocaloric diets (commercial Purina® and AIN-93M), which are considered standard diets, on the accumulation of fat in the liver of rats when offered ad libitum or in a restricted amount. Methods Thus, 40 Wistar rats (90 days old) were separated into 4 groups according to the amount of food offered (ad libitum or dietary restriction) and the type of diet (commercial diet, 3,028.0 kcal/g or AIN-93M, 3,802.7 kcal/g): animals fed the commercial Purina® diet ad libitum (AP), animals fed restricted amounts of the commercial Purina® diet (RP), animals fed the AIN-93M diet ad libitum (AD), and animals fed restricted amounts of the AIN-93M diet (RD). Dietary restriction consisted of pair-feeding the RP and RD groups with 60% of the total food consumed by the corresponding ad libitum groups. Results Because of its higher carbohydrate and calorie content, AIN-93M was found to accelerate weight gain, reduce glucose tolerance and peripheral insulin sensitivity, and increase the amount of fat in the liver when compared to the commercial diet. Conversely, a 40% dietary restriction assisted in weight loss without causing malnutrition, contributing to an improved glucose tolerance and higher levels of HDL cholesterol. Conclusion Therefore, differences in the amount of carbohydrates and calories provided by the diet can lead to important metabolic disorders, such as impaired tolerance and accumulation of hepatic fat, and dietary restriction improves serum and tissue lipid profiles in laboratory animals. PMID:22221448

  7. Heat exchanger-accumulator

    DOEpatents

    Ecker, Amir L.

    1980-01-01

    What is disclosed is a heat exchanger-accumulator for vaporizing a refrigerant or the like, characterized by an upright pressure vessel having a top, bottom and side walls; an inlet conduit eccentrically and sealingly penetrating through the top; a tubular overflow chamber disposed within the vessel and sealingly connected with the bottom so as to define an annular outer volumetric chamber for receiving refrigerant; a heat transfer coil disposed in the outer volumetric chamber for vaporizing the liquid refrigerant that accumulates there; the heat transfer coil defining a passageway for circulating an externally supplied heat exchange fluid; transferring heat efficiently from the fluid; and freely allowing vaporized refrigerant to escape upwardly from the liquid refrigerant; and a refrigerant discharge conduit penetrating sealingly through the top and traversing substantially the length of the pressurized vessel downwardly and upwardly such that its inlet is near the top of the pressurized vessel so as to provide a means for transporting refrigerant vapor from the vessel. The refrigerant discharge conduit has metering orifices, or passageways, penetrating laterally through its walls near the bottom, communicating respectively interiorly and exteriorly of the overflow chamber for controllably carrying small amounts of liquid refrigerant and oil to the effluent stream of refrigerant gas.

  8. [Discussion on acupuncture treatment amount].

    PubMed

    Wu, Song; Liang, Fengxia; Wang, Hua; Li, Jia

    2015-05-01

    The amount of acupuncture treatment refers to the sum of acupuncture effects during the treatment. From the factors that constitutes the amount of acupuncture treatment, including the numbers of needling, the size of acupuncture instruments, the depth of acupuncture, the manipulation, the retained time of needle, etc., and combined with teaching and clinical experience, it is believed that the amount of acupuncture treatment is closely related with clinical efficacy, besides, moderate amount of acupuncture treatment is a key factor to improve acupuncture efficacy and guarantee the safety of acupuncture. The basic principles of regular treatment amount are arrival of qi and being moderate, indicating the retained time of needle, treatment frequency and course should be based on individual condition, time, location and disease. PMID:26255521

  9. The accumulation of nickel in human lungs

    SciTech Connect

    Edelman, D.A.; Roggli, V.L. )

    1989-05-01

    Using data from published studies, lung concentrations of nickel were compare for persons with and without occupational exposure to nickel. As expected, the concentrations were much higher for persons with occupational exposure. To estimate the effects of nickel-containing tobacco smoke and nickel in the ambient air on the amount of nickel accumulated in lungs over time, a model was derived that took into account various variables related to the deposition of nickel in lungs. The model predicted nickel concentrations that were in the range of those of persons without known nickel exposure. Nickel is a suspected carcinogen and has been associated with an increased risk of respiratory tract cancer among nickel workers. However, before the nickel content of cigarettes can be implicated in the etiology of lung cancer, further studies are needed to evaluate the independent effects of smoking and exposure to nickel.

  10. [Eco-environment and nutrient accumulation of sharptooth oak stands in southern slope of Mt. Qinling].

    PubMed

    Liu, Guangquan; Ni, Wenjin; Liu, Huifang; Gravenhorst, G

    2002-05-01

    Based on the date from chemical analysis of 10,785 botanical and 1489 edaphic samples, the amount of nutrients accumulated in soil layer (0-60 cm) of sharptooth oak stands, the total biomass of the stands and their contents of accumulated nutrients, and the amount of litter and its contents of accumulated nutrients in southern slope of Mt. Qinling were calculated. To a certain extent, the biomass and the nutrient accumulation of the stands were increased with stand densities and stand ages from thin to high or from young to old, similar to the utilization efficiency of the stands to soil nutrient elements and the trunk canopy ratio. The tree composition of the stands much more influenced nutrient accumulation contents than the biomass. The more broad-leaved tree of species composition, the higher nutrient accumulation contents of stands. In the same region biomass and its nutrient contents of the stands with similar site factors and stand factors had not remarkably difference among different forest-types, but had a difference among various forest types and species composition. In different region, biomass and its nutrient contents of the stands with similar site factors and stand factors had a difference with the sequence of south slope > north slope, middle section > east section or west section. The utilization efficiency of soil nutrient elements by the stands was decreased from lower to higher elevation, from the south to north slope, from middle to east and west section. PMID:12181886

  11. Pensions and Household Wealth Accumulation

    ERIC Educational Resources Information Center

    Engelhardt, Gary V.; Kumar, Anil

    2011-01-01

    Economists have long suggested that higher private pension benefits "crowd out" other sources of household wealth accumulation. We exploit detailed information on pensions and lifetime earnings for older workers in the 1992 wave of the Health and Retirement Study and employ an instrumental-variable (IV) identification strategy to estimate…

  12. 10 CFR 205.286 - Limitations on amount of refunds.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 3 2011-01-01 2011-01-01 false Limitations on amount of refunds. 205.286 Section 205.286 Energy DEPARTMENT OF ENERGY OIL ADMINISTRATIVE PROCEDURES AND SANCTIONS Special Procedures for... to be remitted pursuant to the relevant DOE enforcement order, plus any accumulated interest,...

  13. 10 CFR 205.286 - Limitations on amount of refunds.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 3 2010-01-01 2010-01-01 false Limitations on amount of refunds. 205.286 Section 205.286 Energy DEPARTMENT OF ENERGY OIL ADMINISTRATIVE PROCEDURES AND SANCTIONS Special Procedures for... to be remitted pursuant to the relevant DOE enforcement order, plus any accumulated interest,...

  14. Effect of minerals on accumulation of Cs by fungus Saccaromyces cerevisiae.

    PubMed

    Ohnuki, Toshihiko; Sakamoto, Fuminori; Yamasaki, Shinya; Kozai, Naofumi; Shiotsu, Hiroyuki; Utsunomiya, Satoshi; Watanabe, Naoko; Kozaki, Tamotsu

    2015-06-01

    The accumulation of Cs by unicellular fungus of Saccharomyces cerevisiae in the presence of minerals has been studied to elucidate the role of microorganisms in the migration of radioactive Cs in the environment. Two different types of experiments were employed: experiments using stable Cs to examine the effect of a carbon source on the accumulation of Cs, and accumulation experiments of radioactive Cs from agar medium containing (137)Cs and zeolite, vermiculite, phlogopite, smectite, mica, or illite as mineral supplements. In the former type of experiments, the Cs-accumulated cells were analyzed by scanning electron microscopy equipped with energy dispersive X-ray analysis (SEM-EDS). In the latter type, the radioactivity in the yeast cells was measured by an autoradiography technique. When a carbon source was present, higher amounts of Cs accumulated in the cells than in the resting condition without a carbon source. Analyses with SEM-EDS showed that no mineral formed on the cell surface. These results indicate that the yeast cells accumulate Cs by adsorption on the cell surface and intracellular accumulation. In the presence of minerals in the agar medium, the radioactivity in the yeast cells was in the order of mica > smectite, illite > vermiculite, phlogopite, zeolite. This order is inversely correlated to the ratio of the concentration of radioactive Cs between the minerals and the medium solution. These results strongly suggest that the yeast accumulates radioactive Cs competitively with minerals. PMID:25841115

  15. ACCUMULATION OF RADIOCESIUM BY MUSHROOMS IN THE ENVIRONMENT: A LITERATURE REVIEW

    SciTech Connect

    Duff, M

    2007-05-28

    During the last 50 years, a large amount of information on radionuclide accumulators or ''sentinel-type'' organisms in the environment has been published. Much of this work focused on the risks of food-chain transfer of radionuclides to higher organisms such as reindeer and man. However, until the 1980's and 1990's, there has been little published data on the radiocesium ({sup 134}Cs and {sup 137}Cs) accumulation by mushrooms. This presentation will consist of a review of the published data for {sup 134,137}Cs accumulation by mushrooms in nature. This review will discuss the aspects that promote {sup 134,137}Cs uptake by mushrooms and focus on mushrooms that demonstrate a large propensity for use in the environmental biomonitoring of radiocesium contamination. It will also provide descriptions of habitats for many of these mushrooms and discuss on how growth media and other conditions relate to Cs accumulation.

  16. Concentration is not enough to evaluate accumulation of heavy metals and nutrients in plants.

    PubMed

    Vymazal, Jan

    2016-02-15

    Wetland plants produce high aboveground biomass and possess the ability to accumulate heavy metals and nutrients. This ability is used for phytoremediation purposes including removal of nutrients and heavy metals from polluted waters. The concentrations of heavy metals are usually much higher in the belowground then in aboveground biomass, especially in roots which are primary sites of uptake. This may lead to the conclusion that accumulation of heavy metals is higher in the belowground biomass. However, in case the aboveground is much higher than belowground biomass the accumulation could be higher in the aboveground biomass. Concentration of nitrogen and phosphorus is always higher in leaves than in stems. However, the stem biomass is often much higher in robust emergent species such as Phragmites australis and therefore, more nutrients can be stored in stems. The examples shown in this communication clearly reveal that to evaluate properly the accumulation of heavy metals and nutrients in particular plant compartment biomass amount must be taken into consideration. In the first study, concentrations of Cd, Cr and Hg in Phalaris arundinacea belowground/aboveground biomass were 150/80μg/kg, 5420/228μg/kg and 38/18μg/kg. The high aboveground biomass (1196g/m(2)) and low belowground biomass (244g/(2)) resulted in much higher accumulation of Cd and Hg in aboveground biomass (96μg/m(2) and 21.2μg/m(2), respectively) than in belowground biomass (36μg/m(2) and 9.3μg/m(2), respectively). Only for chromium, belowground accumulation (1312μg/m(2)) was higher than aboveground accumulation (272μg/m(2)). In the second study, both nitrogen and phosphorus concentrations were higher (26.7mg/g and 749mg/kg, respectively) in leaves than in stems (8.2mg/g and 534mg/kg, respectively) of P. australis. The higher biomass of stems (1835g/m(2)) than leaves (967g/m(2)) resulted in higher accumulation of nitrogen but lower accumulation of phosphorus in leaves as compared to stems

  17. Cadmium accumulation retard activity of functional components of photo assimilation and growth of rice cultivars amended with vermicompost.

    PubMed

    Sebastian, Abin; Prasad, M N V

    2013-01-01

    Cadmium (Cd) uptake mediated alterations in functional components of photo assimilation during conversion of cow dung and poultry cast to vermicompost were studied in two Indian rice cultivars; MO 16 and MTU 7029. It was found that higher amount of Cd accumulate in plants grown in soil amended with vermicompost which in turn damaged functional components in photo assimilation. Enhancement of root growth was recognized as reason for Cd accumulation. Metabolic alterations noticed among plants were not taken place during application of raw materials used for vermicomposting such as cow dung and poultry cast amendment. Rice varieties accumulated Cd differentially where MTU 7029 accumulated more Cd compare to MO 16. It was also noticed that existence of negative correlation between zinc status of the plant and Cd accumulation. PMID:23819289

  18. Radiocesium accumulation properties of Chengiopanax sciadophylloides.

    PubMed

    Sugiura, Yuki; Kanasashi, Tsutomu; Ogata, Yoshimune; Ozawa, Hajime; Takenaka, Chisato

    2016-01-01

    Through the assessments of radioactive contamination after the Fukushima Dai-ichi Nuclear Power Plant (F1NPP) accident, it has been reported that some sprouts of Chengiopanax sciadophylloides (Franch. et Sav.) at the site contained radiocesium (((134),)(137)Cs) at higher concentrations than the other plants. To assess the phytoremediation properties of C. sciadophylloides for (137)Cs decontamination, we aimed to quantify the (137)Cs accumulation in C. sciadophylloides. We measured the (137)Cs concentrations in various organs of C. sciadophylloides collected from the forest in the town of Kawamata, Fukushima prefecture, together with the concentrations of other elements [potassium (K), rubidium, (133)Cs, calcium, strontium, and manganese] present. In addition, we compared the foliar concentrations of these elements in C. sciadophylloides with those in four different deciduous tree species. The mean of foliar (137)Cs concentration in C. sciadophylloides was 28.1 kBq kg(-1) DW, one order of magnitude higher than that found in the other species. The (137)Cs concentrations were in the order of leaves > bark > wood. The wood of the treetop, leaf scars, and roots contained higher amounts of (137)Cs than that of the trunk. From the distribution of (137)Cs in C. sciadophylloides, we confirmed that (137)Cs tends to accumulate in the young growing parts. The difference in the distribution of (137)Cs and (133)Cs indicated that surface uptake of (137)Cs occurs. A significant correlation between K and (137)Cs concentrations in each organ was found, which suggested that (137)Cs in the plant body is transferred through the same pathway as K. On the other hand, there was no correlation between foliar K and (137)Cs concentrations, implying that the uptake ratio of K to (137)Cs was different for each individual. To determine the factors driving specific (137)Cs accumulation and/or the variability of the ratio between K and (137)Cs, the distribution of (137)Cs and the root in soil

  19. Quantitative trait loci controlling amounts and types of epicuticular waxes in onion

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Natural variation exists in onion (Allium cepa L.) for amounts and types of epicuticular waxes on leaves. Wild-type waxy onion possesses copious amounts of these waxes, while the foliage of semi-glossy and glossy phenotypes accumulate significantly less wax. Reduced amounts of epicuticular waxes hav...

  20. Solar-Panel Dust Accumulation and Cleanings

    NASA Technical Reports Server (NTRS)

    2005-01-01

    Air-fall dust accumulates on the solar panels of NASA's Mars Exploration Rovers, reducing the amount of sunlight reaching the solar arrays. Pre-launch models predicted steady dust accumulation. However, the rovers have been blessed with occasional wind events that clear significant amounts of dust from the solar panels.

    This graph shows the effects of those panel-cleaning events on the amount of electricity generated by Spirit's solar panels. The horizontal scale is the number of Martian days (sols) after Spirit's Jan. 4, 2005, (Universal Time) landing on Mars. The vertical scale indicates output from the rover's solar panels as a fraction of the amount produced when the clean panels first opened. Note that the gradual declines are interrupted by occasional sharp increases, such as a dust-cleaning event on sol 420.

  1. Fast carry accumulator design

    NASA Technical Reports Server (NTRS)

    Mastin, W. C.

    1971-01-01

    Simple iterative accumulator combined with gated-carry, carry-completion detection, and skip-carry circuits produces three accumulators with decreased carry propagation times. Devices are used in machine control, measurement equipment, and computer applications to increase speed of binary addition. NAND gates are used in combining network.

  2. Lipid accumulation by oleaginous and non-oleaginous yeast strains in nitrogen and phosphate limitation.

    PubMed

    Kolouchová, Irena; Maťátková, Olga; Sigler, Karel; Masák, Jan; Řezanka, Tomáš

    2016-09-01

    We investigated the possibility of utilizing both oleaginous yeast species accumulating large amounts of lipids (Yarrowia lipolytica, Rhodotorula glutinis, Trichosporon cutaneum, Candida sp.) and traditional biotechnological non-oleaginous ones characterized by high biomass yield (Kluyveromyces polysporus, Torulaspora delbrueckii, Saccharomyces cerevisiae) as potential producers of biofuel-utilizable and nutritionally valuable lipids. The main objective was to increase lipid accumulation by increasing C/P ratio together with higher C/N ratio, while maintaining high biomass yield. The C/N ratio of 30 was found to lead to higher biomass content and the total lipid content increased significantly with higher C/P ratio. With higher ratios of both C/N and C/P, the content of monounsaturated fatty acids (FAs) in cell lipids increased while polyunsaturated FAs decreased. Oleaginous yeast species had a lower proportion of unsaturated FAs (approx. 80 %) than non-oleaginous strains (approx. 90 %). At a C/N ratio of 30 and C/P ratio 1043, T. cutaneum produced a high amount of ω-6 unsaturated linoleic acid, the precursor of some prostaglandins, leukotrienes, and thromboxanes, while Candida sp. and K. polysporus accumulated a high content of palmitoleic acid. PMID:26931336

  3. Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species.

    PubMed

    Mizuno, Yusuke; Sato, Atsushi; Watanabe, Koichi; Hirata, Aiko; Takeshita, Tsuyoshi; Ota, Shuhei; Sato, Norihiro; Zachleder, Vilém; Tsuzuki, Mikio; Kawano, Shigeyuki

    2013-02-01

    The influence of sulfur deficiency on biomass production was analyzed in the four Chlorellaceae species, Chlorella vulgaris, Chlorella sorokiniana, Chlorella lobophora, and Parachlorella kessleri. Culturing under sulfur-deficient conditions promoted transient accumulation of starch followed by a steady increase in lipid storage. Transmission electron microscopy indicated an increase and decrease in starch granules and subsequent enlargement of lipid droplets under sulfur-deficient conditions. Chlorellaceae spp. accumulated 1.5-2.7-fold higher amounts of starch and 1.5-2.4-fold higher amounts of lipid under sulfur-deficient conditions than under sulfur-sufficient conditions. More than 75% of the fatty acids that accumulated in Chlorellaceae spp. under the sulfur-sufficient condition were unsaturated and culturing under sulfur-deficient conditions increased the saturated fatty acid content from 24.3% to 59.7% only in P. kessleri. These results indicate that the sequential accumulation of starch and lipid is a response to the sulfur depletion that commonly occurs in Chlorellaceae spp. PMID:23238344

  4. Bromine accumulation in acidic black colluvial soils

    NASA Astrophysics Data System (ADS)

    Cortizas, Antonio Martínez; Vázquez, Cruz Ferro; Kaal, Joeri; Biester, Harald; Casais, Manuela Costa; Rodríguez, Teresa Taboada; Lado, Luis Rodríguez

    2016-02-01

    Recent investigations showed that bromine is incorporated to soil organic matter (SOM), its content increasing with humification. But few research was done on its long-term accumulation and the role played by pedogenetic processes, as those involved in organic matter stabilization. We investigated bromine content and distribution in four deep, acidic, organic-rich, Holocene soils from an oceanic area of Western Europe. Bromine concentrations (93-778 μg g-1) in the silt + clay (<50 μm) fraction were on average 3-times higher than those (17-250 μg g-1) in the fine earth (<2 mm), the former containing almost all bromine (90 ± 5%). Inventories were between 148 and 314 g m-2, indicating a rather large variability in a small area, and total estimated retention was low (6-16%). The degree of SOM bromination, expressed as the Br/C molar ratio, varied between 0.03 and 1.20 mmol Br/mol C. The ratio was highly correlated (n = 23, r2 0.88, p < 0.01) with the age of the SOM for the last ∼12 ka. Partial least squares modeling indicates that bromine concentration depends on the amount of organic matter stabilized as aluminium-OM associations, and to a lesser extent on soil acidity (pH) and iron-OM associations. Thus, at scales of thousands of years, bromine accumulation in acidic soils is linked to the pool of metal-clay-stabilized organic matter.

  5. 46 CFR Sec. 2 - Amount of bond.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Amount of bond. Sec. 2 Section 2 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY BONDING OF SHIP'S PERSONNEL Sec. 2 Amount of bond. The amount of the bond must be governed by the amount of monies advanced or value of...

  6. 46 CFR Sec. 2 - Amount of bond.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 8 2011-10-01 2011-10-01 false Amount of bond. Sec. 2 Section 2 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL SHIPPING AUTHORITY BONDING OF SHIP'S PERSONNEL Sec. 2 Amount of bond. The amount of the bond must be governed by the amount of monies advanced or value of...

  7. 47 CFR 36.503 - Accumulated depreciation-Account 3100.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Accumulated depreciation-Account 3100. 36.503... Accumulated depreciation—Account 3100. (a) Amounts recorded in this account shall be separated on the basis of the separation of the associated primary Plant Accounts or related categories, excluding...

  8. 29 CFR 4219.13 - Amount of liability for de minimis amounts.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Amount of liability for de minimis amounts. 4219.13 Section... Redetermination of Withdrawal Liability Upon Mass Withdrawal § 4219.13 Amount of liability for de minimis amounts. An employer that is liable for de minimis amounts shall be liable to the plan for the amount by...

  9. 75 FR 58285 - Short-Term, Small Amount Loans

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-24

    ...NCUA is amending its general lending rule to enable Federal credit unions (FCUs) to offer short-term, small amount loans (STS loans) as a viable alternative to predatory payday loans. The amendment permits FCUs to charge a higher interest rate for an STS loan than is permitted under the general lending rule, but imposes limitations on the permissible term, amount, and fees associated with an......

  10. Neurodegeneration with Brain Iron Accumulation

    MedlinePlus

    ... Diversity Find People About NINDS NINDS Neurodegeneration with Brain Iron Accumulation Information Page Synonym(s): Hallervorden-Spatz Disease, ... done? Clinical Trials Organizations What is Neurodegeneration with Brain Iron Accumulation? Neurodegeneration with brain iron accumulation (NBIA) ...

  11. Plastids and Carotenoid Accumulation.

    PubMed

    Li, Li; Yuan, Hui; Zeng, Yunliu; Xu, Qiang

    2016-01-01

    Plastids are ubiquitously present in plants and are the organelles for carotenoid biosynthesis and storage. Based on their morphology and function, plastids are classified into various types, i.e. proplastids, etioplasts, chloroplasts, amyloplasts, and chromoplasts. All plastids, except proplastids, can synthesize carotenoids. However, plastid types have a profound effect on carotenoid accumulation and stability. In this chapter, we discuss carotenoid biosynthesis and regulation in various plastids with a focus on carotenoids in chromoplasts. Plastid transition related to carotenoid biosynthesis and the different capacity of various plastids to sequester carotenoids and the associated effect on carotenoid stability are described in light of carotenoid accumulation in plants. PMID:27485226

  12. Effects of perchlorate on growth of four wetland plants and its accumulation in plant tissues.

    PubMed

    He, Hongzhi; Gao, Haishuo; Chen, Guikui; Li, Huashou; Lin, Hai; Shu, Zhenzhen

    2013-10-01

    Perchlorate contamination in water is of concern because of uncertainties about toxicity and health effects, impact on ecosystems, and possible indirect exposure pathways to humans. Therefore, it is very important to investigate the ecotoxicology of perchlorate and to screen plant species for phytoremediation. Effects of perchlorate (20, 200, and 500 mg/L) on the growth of four wetland plants (Eichhornia crassipes, Acorus calamus L., Thalia dealbata, and Canna indica) as well as its accumulation in different plant tissues were investigated through water culture experiments. Twenty milligrams per liter of perchlorate had no significant effects on height, root length, aboveground part weight, root weight, and oxidizing power of roots of four plants, except A. calamus, and increasing concentrations of perchlorate showed that out of the four wetland plants, only A. calamus had a significant (p<0.05) dose-dependent decrease in these parameters. When treated with 500 mg/L perchlorate, these parameters and chlorophyll content in the leaf of plants showed significant decline contrasted to control groups, except the root length of E. crassipes and C. indica. The order of inhibition rates of perchlorate on root length, aboveground part weight and root weight, and oxidizing power of roots was: A. calamus > C. indica > T. dealbata > E. crassipes and on chlorophyll content in the leaf it was: A. calamus > T. dealbata > C. indica > E. crassipes. The higher the concentration of perchlorate used, the higher the amount of perchlorate accumulation in plants. Perchlorate accumulation in aboveground tissues was much higher than that in underground tissues and leaf was the main tissue for perchlorate accumulation. The order of perchlorate accumulation content and the bioconcentration factor in leaf of four plants was: E. crassipes > C. indica > T. dealbata > A. calamus. Therefore, E. crassipes might be an ideal plant with high tolerance ability and accumulation ability for constructing

  13. 46 CFR 308.403 - Insured amounts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... total amount of war risk insurance obtainable from companies authorized to do an insurance business in a... MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Builder's Risk Insurance § 308.403 Insured amounts. (a) Prelaunching period. The amount insured...

  14. 14 CFR 1261.102 - Maximum amount.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Maximum amount. 1261.102 Section 1261.102 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PROCESSING OF MONETARY CLAIMS (GENERAL) Employees' Personal Property Claims § 1261.102 Maximum amount. From October 1, 1982, to October 30, 1988, the maximum amount that may be...

  15. 14 CFR 1261.102 - Maximum amount.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Maximum amount. 1261.102 Section 1261.102 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PROCESSING OF MONETARY CLAIMS (GENERAL) Employees' Personal Property Claims § 1261.102 Maximum amount. From October 1, 1982, to October 30, 1988, the maximum amount that may be...

  16. THE KINETICS OF PENETRATION : VIII. TEMPORARY ACCUMULATION.

    PubMed

    Osterhout, W J; Kamerling, S E

    1934-03-20

    solution it will tend to enter and displace the weaker acid HA (if this be carbonic it can readily escape): hence potassium may accumulate to a greater or less extent as KCl. Injury of the cell may produce a twofold effect, (1) increase of permeability, (2) lessened accumulation. The total amount of electrolyte taken up in a given time will be influenced by these factors and may be greater than normal in the injured cell or less, depending somewhat on the length of the interval of time chosen. PMID:19872797

  17. Chimpanzee accumulative stone throwing.

    PubMed

    Kühl, Hjalmar S; Kalan, Ammie K; Arandjelovic, Mimi; Aubert, Floris; D'Auvergne, Lucy; Goedmakers, Annemarie; Jones, Sorrel; Kehoe, Laura; Regnaut, Sebastien; Tickle, Alexander; Ton, Els; van Schijndel, Joost; Abwe, Ekwoge E; Angedakin, Samuel; Agbor, Anthony; Ayimisin, Emmanuel Ayuk; Bailey, Emma; Bessone, Mattia; Bonnet, Matthieu; Brazolla, Gregory; Buh, Valentine Ebua; Chancellor, Rebecca; Cipoletta, Chloe; Cohen, Heather; Corogenes, Katherine; Coupland, Charlotte; Curran, Bryan; Deschner, Tobias; Dierks, Karsten; Dieguez, Paula; Dilambaka, Emmanuel; Diotoh, Orume; Dowd, Dervla; Dunn, Andrew; Eshuis, Henk; Fernandez, Rumen; Ginath, Yisa; Hart, John; Hedwig, Daniela; Ter Heegde, Martijn; Hicks, Thurston Cleveland; Imong, Inaoyom; Jeffery, Kathryn J; Junker, Jessica; Kadam, Parag; Kambi, Mohamed; Kienast, Ivonne; Kujirakwinja, Deo; Langergraber, Kevin; Lapeyre, Vincent; Lapuente, Juan; Lee, Kevin; Leinert, Vera; Meier, Amelia; Maretti, Giovanna; Marrocoli, Sergio; Mbi, Tanyi Julius; Mihindou, Vianet; Moebius, Yasmin; Morgan, David; Morgan, Bethan; Mulindahabi, Felix; Murai, Mizuki; Niyigabae, Protais; Normand, Emma; Ntare, Nicolas; Ormsby, Lucy Jayne; Piel, Alex; Pruetz, Jill; Rundus, Aaron; Sanz, Crickette; Sommer, Volker; Stewart, Fiona; Tagg, Nikki; Vanleeuwe, Hilde; Vergnes, Virginie; Willie, Jacob; Wittig, Roman M; Zuberbuehler, Klaus; Boesch, Christophe

    2016-01-01

    The study of the archaeological remains of fossil hominins must rely on reconstructions to elucidate the behaviour that may have resulted in particular stone tools and their accumulation. Comparatively, stone tool use among living primates has illuminated behaviours that are also amenable to archaeological examination, permitting direct observations of the behaviour leading to artefacts and their assemblages to be incorporated. Here, we describe newly discovered stone tool-use behaviour and stone accumulation sites in wild chimpanzees reminiscent of human cairns. In addition to data from 17 mid- to long-term chimpanzee research sites, we sampled a further 34 Pan troglodytes communities. We found four populations in West Africa where chimpanzees habitually bang and throw rocks against trees, or toss them into tree cavities, resulting in conspicuous stone accumulations at these sites. This represents the first record of repeated observations of individual chimpanzees exhibiting stone tool use for a purpose other than extractive foraging at what appear to be targeted trees. The ritualized behavioural display and collection of artefacts at particular locations observed in chimpanzee accumulative stone throwing may have implications for the inferences that can be drawn from archaeological stone assemblages and the origins of ritual sites. PMID:26923684

  18. Chimpanzee accumulative stone throwing

    PubMed Central

    Kühl, Hjalmar S.; Kalan, Ammie K.; Arandjelovic, Mimi; Aubert, Floris; D’Auvergne, Lucy; Goedmakers, Annemarie; Jones, Sorrel; Kehoe, Laura; Regnaut, Sebastien; Tickle, Alexander; Ton, Els; van Schijndel, Joost; Abwe, Ekwoge E.; Angedakin, Samuel; Agbor, Anthony; Ayimisin, Emmanuel Ayuk; Bailey, Emma; Bessone, Mattia; Bonnet, Matthieu; Brazolla, Gregory; Buh, Valentine Ebua; Chancellor, Rebecca; Cipoletta, Chloe; Cohen, Heather; Corogenes, Katherine; Coupland, Charlotte; Curran, Bryan; Deschner, Tobias; Dierks, Karsten; Dieguez, Paula; Dilambaka, Emmanuel; Diotoh, Orume; Dowd, Dervla; Dunn, Andrew; Eshuis, Henk; Fernandez, Rumen; Ginath, Yisa; Hart, John; Hedwig, Daniela; Ter Heegde, Martijn; Hicks, Thurston Cleveland; Imong, Inaoyom; Jeffery, Kathryn J.; Junker, Jessica; Kadam, Parag; Kambi, Mohamed; Kienast, Ivonne; Kujirakwinja, Deo; Langergraber, Kevin; Lapeyre, Vincent; Lapuente, Juan; Lee, Kevin; Leinert, Vera; Meier, Amelia; Maretti, Giovanna; Marrocoli, Sergio; Mbi, Tanyi Julius; Mihindou, Vianet; Moebius, Yasmin; Morgan, David; Morgan, Bethan; Mulindahabi, Felix; Murai, Mizuki; Niyigabae, Protais; Normand, Emma; Ntare, Nicolas; Ormsby, Lucy Jayne; Piel, Alex; Pruetz, Jill; Rundus, Aaron; Sanz, Crickette; Sommer, Volker; Stewart, Fiona; Tagg, Nikki; Vanleeuwe, Hilde; Vergnes, Virginie; Willie, Jacob; Wittig, Roman M.; Zuberbuehler, Klaus; Boesch, Christophe

    2016-01-01

    The study of the archaeological remains of fossil hominins must rely on reconstructions to elucidate the behaviour that may have resulted in particular stone tools and their accumulation. Comparatively, stone tool use among living primates has illuminated behaviours that are also amenable to archaeological examination, permitting direct observations of the behaviour leading to artefacts and their assemblages to be incorporated. Here, we describe newly discovered stone tool-use behaviour and stone accumulation sites in wild chimpanzees reminiscent of human cairns. In addition to data from 17 mid- to long-term chimpanzee research sites, we sampled a further 34 Pan troglodytes communities. We found four populations in West Africa where chimpanzees habitually bang and throw rocks against trees, or toss them into tree cavities, resulting in conspicuous stone accumulations at these sites. This represents the first record of repeated observations of individual chimpanzees exhibiting stone tool use for a purpose other than extractive foraging at what appear to be targeted trees. The ritualized behavioural display and collection of artefacts at particular locations observed in chimpanzee accumulative stone throwing may have implications for the inferences that can be drawn from archaeological stone assemblages and the origins of ritual sites. PMID:26923684

  19. Accumulation of the planets

    NASA Technical Reports Server (NTRS)

    Wetherill, G. W.

    1987-01-01

    In modeling the accumulation of planetesimals into planets, it is appropriate to distinguish between two stages: an early stage, during which approximately 10 km diameter planetesimals accumulate locally to form bodies approximate 10 to the 25th g in mass; and a later stage in which the approximately 10 to the 25th g planetesimals accumulate into the final planets. In the terrestrial planet region, an initial planetesimal swarm corresponding to the critical mass of dust layer gravitational instabilities is considered. In order to better understand the accumulation history of Mercury-sized bodies, 19 Monte-Carlo simulations of terrestrial planet growth were calculated. A Monte Carlo technique was used to investigate the orbital evolution of asteroidal collision debris produced interior to 2.6 AU. It was found that there are two regions primarily responsible for production of Earth-crossing meteoritic material and Apollo objects. The same techniques were extended to include the origin of Earth-approaching asteroidal bodies. It is found that these same two resonant mechanisms predict a steady-state number of Apollo-Amor about 1/2 that estimated based on astronomical observations.

  20. Whole Genome Re-Sequencing Identifies a Quantitative Trait Locus Repressing Carbon Reserve Accumulation during Optimal Growth in Chlamydomonas reinhardtii.

    PubMed

    Goold, Hugh Douglas; Nguyen, Hoa Mai; Kong, Fantao; Beyly-Adriano, Audrey; Légeret, Bertrand; Billon, Emmanuelle; Cuiné, Stéphan; Beisson, Fred; Peltier, Gilles; Li-Beisson, Yonghua

    2016-01-01

    Microalgae have emerged as a promising source for biofuel production. Massive oil and starch accumulation in microalgae is possible, but occurs mostly when biomass growth is impaired. The molecular networks underlying the negative correlation between growth and reserve formation are not known. Thus isolation of strains capable of accumulating carbon reserves during optimal growth would be highly desirable. To this end, we screened an insertional mutant library of Chlamydomonas reinhardtii for alterations in oil content. A mutant accumulating five times more oil and twice more starch than wild-type during optimal growth was isolated and named constitutive oil accumulator 1 (coa1). Growth in photobioreactors under highly controlled conditions revealed that the increase in oil and starch content in coa1 was dependent on light intensity. Genetic analysis and DNA hybridization pointed to a single insertional event responsible for the phenotype. Whole genome re-sequencing identified in coa1 a >200 kb deletion on chromosome 14 containing 41 genes. This study demonstrates that, 1), the generation of algal strains accumulating higher reserve amount without compromising biomass accumulation is feasible; 2), light is an important parameter in phenotypic analysis; and 3), a chromosomal region (Quantitative Trait Locus) acts as suppressor of carbon reserve accumulation during optimal growth. PMID:27141848

  1. Whole Genome Re-Sequencing Identifies a Quantitative Trait Locus Repressing Carbon Reserve Accumulation during Optimal Growth in Chlamydomonas reinhardtii

    PubMed Central

    Goold, Hugh Douglas; Nguyen, Hoa Mai; Kong, Fantao; Beyly-Adriano, Audrey; Légeret, Bertrand; Billon, Emmanuelle; Cuiné, Stéphan; Beisson, Fred; Peltier, Gilles; Li-Beisson, Yonghua

    2016-01-01

    Microalgae have emerged as a promising source for biofuel production. Massive oil and starch accumulation in microalgae is possible, but occurs mostly when biomass growth is impaired. The molecular networks underlying the negative correlation between growth and reserve formation are not known. Thus isolation of strains capable of accumulating carbon reserves during optimal growth would be highly desirable. To this end, we screened an insertional mutant library of Chlamydomonas reinhardtii for alterations in oil content. A mutant accumulating five times more oil and twice more starch than wild-type during optimal growth was isolated and named constitutive oil accumulator 1 (coa1). Growth in photobioreactors under highly controlled conditions revealed that the increase in oil and starch content in coa1 was dependent on light intensity. Genetic analysis and DNA hybridization pointed to a single insertional event responsible for the phenotype. Whole genome re-sequencing identified in coa1 a >200 kb deletion on chromosome 14 containing 41 genes. This study demonstrates that, 1), the generation of algal strains accumulating higher reserve amount without compromising biomass accumulation is feasible; 2), light is an important parameter in phenotypic analysis; and 3), a chromosomal region (Quantitative Trait Locus) acts as suppressor of carbon reserve accumulation during optimal growth. PMID:27141848

  2. Identification of genes associated with chlorophyll accumulation in flower petals.

    PubMed

    Ohmiya, Akemi; Hirashima, Masumi; Yagi, Masafumi; Tanase, Koji; Yamamizo, Chihiro

    2014-01-01

    Plants have an ability to prevent chlorophyll accumulation, which would mask the bright flower color, in their petals. In contrast, leaves contain substantial amounts of chlorophyll, as it is essential for photosynthesis. The mechanisms of organ-specific chlorophyll accumulation are unknown. To identify factors that determine the chlorophyll content in petals, we compared the expression of genes related to chlorophyll metabolism in different stages of non-green (red and white) petals (very low chlorophyll content), pale-green petals (low chlorophyll content), and leaves (high chlorophyll content) of carnation (Dianthus caryophyllus L.). The expression of many genes encoding chlorophyll biosynthesis enzymes, in particular Mg-chelatase, was lower in non-green petals than in leaves. Non-green petals also showed higher expression of genes involved in chlorophyll degradation, including STAY-GREEN gene and pheophytinase. These data suggest that the absence of chlorophylls in carnation petals may be caused by the low rate of chlorophyll biosynthesis and high rate of degradation. Similar results were obtained by the analysis of Arabidopsis microarray data. In carnation, most genes related to chlorophyll biosynthesis were expressed at similar levels in pale-green petals and leaves, whereas the expression of chlorophyll catabolic genes was higher in pale-green petals than in leaves. Therefore, we hypothesize that the difference in chlorophyll content between non-green and pale-green petals is due to different levels of chlorophyll biosynthesis. Our study provides a basis for future molecular and genetic studies on organ-specific chlorophyll accumulation. PMID:25470367

  3. Identification of Genes Associated with Chlorophyll Accumulation in Flower Petals

    PubMed Central

    Ohmiya, Akemi; Hirashima, Masumi; Yagi, Masafumi; Tanase, Koji; Yamamizo, Chihiro

    2014-01-01

    Plants have an ability to prevent chlorophyll accumulation, which would mask the bright flower color, in their petals. In contrast, leaves contain substantial amounts of chlorophyll, as it is essential for photosynthesis. The mechanisms of organ-specific chlorophyll accumulation are unknown. To identify factors that determine the chlorophyll content in petals, we compared the expression of genes related to chlorophyll metabolism in different stages of non-green (red and white) petals (very low chlorophyll content), pale-green petals (low chlorophyll content), and leaves (high chlorophyll content) of carnation (Dianthus caryophyllus L.). The expression of many genes encoding chlorophyll biosynthesis enzymes, in particular Mg-chelatase, was lower in non-green petals than in leaves. Non-green petals also showed higher expression of genes involved in chlorophyll degradation, including STAY-GREEN gene and pheophytinase. These data suggest that the absence of chlorophylls in carnation petals may be caused by the low rate of chlorophyll biosynthesis and high rate of degradation. Similar results were obtained by the analysis of Arabidopsis microarray data. In carnation, most genes related to chlorophyll biosynthesis were expressed at similar levels in pale-green petals and leaves, whereas the expression of chlorophyll catabolic genes was higher in pale-green petals than in leaves. Therefore, we hypothesize that the difference in chlorophyll content between non-green and pale-green petals is due to different levels of chlorophyll biosynthesis. Our study provides a basis for future molecular and genetic studies on organ-specific chlorophyll accumulation. PMID:25470367

  4. The amount effect and marginal value.

    PubMed

    Rachlin, Howard; Arfer, Kodi B; Safin, Vasiliy; Yen, Ming

    2015-07-01

    The amount effect of delay discounting (by which the value of larger reward amounts is discounted by delay at a lower rate than that of smaller amounts) strictly implies that value functions (value as a function of amount) are steeper at greater delays than they are at lesser delays. That is, the amount effect and the difference in value functions at different delays are actually a single empirical finding. Amount effects of delay discounting are typically found with choice experiments. Value functions for immediate rewards have been empirically obtained by direct judgment. (Value functions for delayed rewards have not been previously obtained.) The present experiment obtained value functions for both immediate and delayed rewards by direct judgment and found them to be steeper when the rewards were delayed--hence, finding an amount effect with delay discounting. PMID:26013159

  5. Heterologous expression of Ceratophyllum demersum phytochelatin synthase, CdPCS1, in rice leads to lower arsenic accumulation in grain

    PubMed Central

    Shri, Manju; Dave, Richa; Diwedi, Sanjay; Shukla, Devesh; Kesari, Ravi; Tripathi, Rudra Deo; Trivedi, Prabodh Kumar; Chakrabarty, Debasis

    2014-01-01

    Recent studies have identified rice (Oryza sativa) as a major dietary source of inorganic arsenic (As) and poses a significant human health risk. The predominant model for plant detoxification of heavy metals is complexation of heavy metals with phytochelatins (PCs), synthesized non-translationally by PC synthase (PCS) and compartmentalized in vacuoles. In this study, in order to restrict As in the rice roots as a detoxification mechanism, a transgenic approach has been followed through expression of phytochelatin synthase, CdPCS1, from Ceratophyllum demersum, an aquatic As-accumulator plant. CdPCS1 expressing rice transgenic lines showed marked increase in PCS activity and enhanced synthesis of PCs in comparison to non-transgenic plant. Transgenic lines showed enhanced accumulation of As in root and shoot. This enhanced metal accumulation potential of transgenic lines was positively correlated to the content of PCs, which also increased several-fold higher in transgenic lines. However, all the transgenic lines accumulated significantly lower As in grain and husk in comparison to non-transgenic plant. The higher level of PCs in transgenic plants relative to non-transgenic presumably allowed sequestering and detoxification of higher amounts of As in roots and shoots, thereby restricting its accumulation in grain. PMID:25048298

  6. Heterologous expression of Ceratophyllum demersum phytochelatin synthase, CdPCS1, in rice leads to lower arsenic accumulation in grain.

    PubMed

    Shri, Manju; Dave, Richa; Diwedi, Sanjay; Shukla, Devesh; Kesari, Ravi; Tripathi, Rudra Deo; Trivedi, Prabodh Kumar; Chakrabarty, Debasis

    2014-01-01

    Recent studies have identified rice (Oryza sativa) as a major dietary source of inorganic arsenic (As) and poses a significant human health risk. The predominant model for plant detoxification of heavy metals is complexation of heavy metals with phytochelatins (PCs), synthesized non-translationally by PC synthase (PCS) and compartmentalized in vacuoles. In this study, in order to restrict As in the rice roots as a detoxification mechanism, a transgenic approach has been followed through expression of phytochelatin synthase, CdPCS1, from Ceratophyllum demersum, an aquatic As-accumulator plant. CdPCS1 expressing rice transgenic lines showed marked increase in PCS activity and enhanced synthesis of PCs in comparison to non-transgenic plant. Transgenic lines showed enhanced accumulation of As in root and shoot. This enhanced metal accumulation potential of transgenic lines was positively correlated to the content of PCs, which also increased several-fold higher in transgenic lines. However, all the transgenic lines accumulated significantly lower As in grain and husk in comparison to non-transgenic plant. The higher level of PCs in transgenic plants relative to non-transgenic presumably allowed sequestering and detoxification of higher amounts of As in roots and shoots, thereby restricting its accumulation in grain. PMID:25048298

  7. Is There Neural Evidence for an Evidence Accumulation Process in Memory Decisions?

    PubMed Central

    van Vugt, Marieke K.; Beulen, Marijke A.; Taatgen, Niels A.

    2016-01-01

    Models of evidence accumulation have been very successful at describing human decision making behavior. Recent years have also seen the first reports of neural correlates of this accumulation process. However, these studies have mostly focused on perceptual decision making tasks, ignoring the role of additional cognitive processes like memory retrieval that are crucial in real-world decisions. In this study, we tried to find a neural signature of evidence accumulation during a recognition memory task. To do this, we applied a method we have successfully used to localize evidence accumulation in scalp EEG during a perceptual decision making task. This time, however, we applied it to intracranial EEG recordings, which provide a much higher spatial resolution. We identified several brain areas where activity ramps up over time, but these neural patterns do not appear to be modulated by behavioral variables such as the amount of available evidence or response time. This casts doubt on the idea of evidence accumulation as a general decision-making mechanism underlying different types of decisions. PMID:27014024

  8. Interaction between sulfur and lead in toxicity, iron plaque formation and lead accumulation in rice plant.

    PubMed

    Yang, Junxing; Liu, Zhiyan; Wan, Xiaoming; Zheng, Guodi; Yang, Jun; Zhang, Hanzhi; Guo, Lin; Wang, Xuedong; Zhou, Xiaoyong; Guo, Qingjun; Xu, Ruixiang; Zhou, Guangdong; Peters, Marc; Zhu, Guangxu; Wei, Rongfei; Tian, Liyan; Han, Xiaokun

    2016-06-01

    Human activities have resulted in lead and sulfur accumulation in paddy soils in parts of southern China. A combined soil-sand pot experiment was conducted to investigate the influence of S supply on iron plaque formation and Pb accumulation in rice (Oryza sativa L.) under two Pb levels (0 and 600 mg kg(-1)), combined with four S concentrations (0, 30, 60, and 120 mg kg(-1)). Results showed that S supply significantly decreased Pb accumulation in straw and grains of rice. This result may be attributed to the enhancement of Fe plaque formation, decrease of Pb availability in soil, and increase of reduced glutathione (GSH) in rice leaves. Moderate S supply (30 mg kg(-1)) significantly increased Fe plaque formation on the root surface and in the rhizosphere, whereas excessive S supply (60 and 120 mg kg(-1)) significantly decreased the amounts of iron plaque on the root surface. Sulfur supply significantly enhanced the GSH contents in leaves of rice plants under Pb treatment. With excessive S application, the rice root acted as a more effective barrier to Pb accumulation compared with iron plaque. Excessive S supply may result in a higher monosulfide toxicity and decreased iron plaque formation on the root surface during flooded conditions. However, excessive S supply could effectively decrease Pb availability in soils and reduce Pb accumulation in rice plants. PMID:26946285

  9. Checking ozone amounts by measurements of UV-irradiances

    NASA Technical Reports Server (NTRS)

    Seckmeyer, Gunther; Kettner, Christiane; Thiel, Stephen

    1994-01-01

    Absolute measurements of UV-irradiances in Germany and New Zealand are used to determine the total amounts of ozone. UV-irradiances measured and calculated for clear skies and for solar zenith angles less than 60 deg generally show a good accordance. The UVB-irradiances, however, show that the actual Dobson values are about 5 percent higher in Germany and about 3 percent higher in New Zealand compared to those obtained by our method. Possible reasons for these deviations are discussed.

  10. [Effects of sprinkler irrigation on the plant nitrogen accumulation and translocation and kernel protein content of winter wheat].

    PubMed

    Yao, Su-mei; Kang, Yue-hu; Ru, Zhen-gang; Liu, Ming-jiu; Yang, Wen-ping; Li, Gan

    2013-08-01

    Taking wheat cultivar Bainong AK58 as test material, a field experiment was conducted to study the plant nitrogen accumulation and translocation and kernel protein content of winter wheat under sprinkler irrigation and surface irrigation, aimed to understand the differences in the nitrogen metabolism characteristics of winter wheat under different irrigation regimes. At booting stage, no significant difference was observed in the total amount of plant nitrogen accumulation between sprinkler irrigation and surface irrigation; while from booting stage to maturing stage, the total amount of plant nitrogen accumulation under sprinkler irrigation was significantly higher. Under sprinkler irrigation, the translocation amount and contribution rate of the nitrogen stored in leaf, glume, stem and sheath at pre-anthesis to the kernel increased, while the contribution rate of the assimilated nitrogen after anthesis to the kernel nitrogen declined. Both the relative protein content and the total protein yield in the kernel increased significantly under sprinkler irrigation. In conclusion, sprinkler irrigation could significantly regulate the nitrogen translocation and kernel protein accumulation of winter wheat. PMID:24380339

  11. Effects of water deficit on radicle apex elongation and solute accumulation in Zea mays L.

    PubMed

    Velázquez-Márquez, S; Conde-Martínez, V; Trejo, C; Delgado-Alvarado, A; Carballo, A; Suárez, R; Mascorro, J O; Trujillo, A R

    2015-11-01

    In this study, we examined the effects of water deficit on the elongation of radicles of maize seedlings and on the accumulation of solutes in the radicle apices of two maize varieties: VS-22 (tolerant) and AMCCG-2 (susceptible). Sections of radicle corresponding to the first 2 mm of the primary roots were marked with black ink, and the seedlings were allowed to grow for 24, 48, and 72 h in polyvinyl chloride (PVC) tubes filled with vermiculite at three different water potentials (Ψ(w), -0.03, -1.0, and -1.5 MPa). The radicle elongation, sugar accumulation, and proline accumulation were determined after each of the growth periods specified above. The Ψ(w) of the substrate affected the dynamics of primary root elongation in both varieties. In particular, the lowest Ψ(w) (-1.5 MPa) inhibited root development by 72% and 90% for the VS-22 and AMCCG-2 varieties, respectively. The osmotic potential (Ψ(o)) was reduced substantially in both varieties to maintain root turgor; however, VS-22 had a higher root turgor (0.67 MPa) than AMCCG-2 (0.2 MPa). These results suggest that both varieties possess a capacity for osmotic adjustment. Sugar began to accumulate within the first 24 h of radicle apex growth. The sugar concentration was higher in VS-22 root apices compared to AMCCG-2, and the amount of sugar accumulation increased with a decrease in Ψ(w). Significant amounts of trehalose accumulated in VS-22 and AMCCG-2 (29.8 μmol/g fresh weight [FW] and 5.24 μmol/g FW, respectively). Starch accumulation in the root apices of these two maize varieties also differed significantly, with a lower level in VS-22. In both varieties, the proline concentration also increased as a consequence of the water deficit. At 72 h, the proline concentration in VS-22 (16.2 μmol/g FW) was almost 3 times greater than that in AMCCG-2 (5.19 μmol/g FW). Trehalose also showed a 3-fold increase in the tolerant variety. Accumulation of these solutes in the root growth zone may indicate an osmotic

  12. 20 CFR 725.533 - Modification of benefits amounts; general.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... a payment during the month of filing, or a dependent qualifies under this part for an augmentation... third decimal is raised to the next higher multiple of 10 cents. (e) Any individual entitled to a... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Modification of benefits amounts;...

  13. Maturation of Rb+ and PAH accumulation by rabbit anterior uvea and choroid plexus

    SciTech Connect

    Krupin, T.; Fritz, C.; Becker, B.

    1985-02-01

    In vitro accumulation of radioactive para-aminohippuric acid (/sup 3/H-PAH) and rubidium (/sup 86/Rb+) by the anterior uvea, ciliary processes, and the choroid plexus was evaluated in tissues from newborn and various aged rabbits. Accumulation of PAH was present in the anterior uvea at 1 day of age (tissue to media ratio, T/M, of 2.1 +/- 0.2) and remained at this level for the first 14 days of life. Accumulation did not rise to adult levels until 21 days of age (T/M 5.5 +/- 0.6). Rubidium accumulation in the anterior uvea, a measure of Na+, K+-pump activity, was higher than adult values 6 hr after birth (T/M25.2 +/- 0.9). Activity remained elevated through day 28 and did not fall to adult levels until day 60 (T/M 13.4 +/- 0.6). Accumulation studies on isolated ciliary processes were similar to those obtained from anterior uveal tissue. Daily subcutaneous injections of penicillin (300,000 units/kg/day) for 1 week had no effect on anterior uvea PAH accumulation (penicillin T/M was 1.7 +/- 0.1 and saline control T/M was 2.0 +/- 0.2). Accumulation of either /sup 3/H-PAH or /sup 86/Rb+ by the choroid plexus was present 1 day after birth in amounts that were similar to adult values and did not change during the 90 days of testing.

  14. 13 CFR 400.202 - Loan amount.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ....202 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.202 Loan amount. (a) The aggregate amount of loan principal guaranteed under this Program to a single Qualified Steel Company may not exceed $ 250 million. (b) Of...

  15. 13 CFR 400.202 - Loan amount.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ....202 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.202 Loan amount. (a) The aggregate amount of loan principal guaranteed under this Program to a single Qualified Steel Company may not exceed $ 250 million. (b) Of...

  16. 13 CFR 400.202 - Loan amount.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ....202 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.202 Loan amount. (a) The aggregate amount of loan principal guaranteed under this Program to a single Qualified Steel Company may not exceed $ 250 million. (b) Of...

  17. 13 CFR 400.202 - Loan amount.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....202 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.202 Loan amount. (a) The aggregate amount of loan principal guaranteed under this Program to a single Qualified Steel Company may not exceed $ 250 million. (b) Of...

  18. 13 CFR 400.202 - Loan amount.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ....202 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.202 Loan amount. (a) The aggregate amount of loan principal guaranteed under this Program to a single Qualified Steel Company may not exceed $ 250 million. (b) Of...

  19. 23 CFR 1335.8 - Grant amounts.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Grant amounts. 1335.8 Section 1335.8 Highways NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION STATE HIGHWAY SAFETY DATA IMPROVEMENTS § 1335.8 Grant amounts. (a) Start-up grant. A State that qualifies for a start-up grant under §...

  20. 45 CFR 32.8 - Amounts withheld.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... debtor's disposable pay exceeds an amount equivalent to thirty times the minimum wage. See 29 CFR 870.10... DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION ADMINISTRATIVE WAGE GARNISHMENT § 32.8 Amounts... first pay day after the employer receives the order. However, if the first pay day is within 10...

  1. 45 CFR 32.8 - Amounts withheld.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... debtor's disposable pay exceeds an amount equivalent to thirty times the minimum wage. See 29 CFR 870.10... DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION ADMINISTRATIVE WAGE GARNISHMENT § 32.8 Amounts... first pay day after the employer receives the order. However, if the first pay day is within 10...

  2. 14 CFR 1261.102 - Maximum amount.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Maximum amount. 1261.102 Section 1261.102 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PROCESSING OF MONETARY CLAIMS (GENERAL) Employees' Personal Property Claims § 1261.102 Maximum amount. From October 1, 1982, to October 30,...

  3. 14 CFR 1261.102 - Maximum amount.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Maximum amount. 1261.102 Section 1261.102 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PROCESSING OF MONETARY CLAIMS (GENERAL) Employees' Personal Property Claims § 1261.102 Maximum amount. From October 1, 1982, to October 30,...

  4. 45 CFR 32.8 - Amounts withheld.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... debtor's disposable pay exceeds an amount equivalent to thirty times the minimum wage. See 29 CFR 870.10... DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION ADMINISTRATIVE WAGE GARNISHMENT § 32.8 Amounts... first pay day after the employer receives the order. However, if the first pay day is within 10...

  5. 20 CFR 617.34 - Amount.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... the Federal travel regulations (see 41 CFR part 101-7) for the locality where the job search is... WORKERS UNDER THE TRADE ACT OF 1974 Job Search Allowances § 617.34 Amount. (a) Computation. The amount of a job search allowance shall be 90 percent of the total costs of each of the following...

  6. 20 CFR 617.34 - Amount.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... the Federal travel regulations (see 41 CFR part 101-7) for the locality where the job search is... WORKERS UNDER THE TRADE ACT OF 1974 Job Search Allowances § 617.34 Amount. (a) Computation. The amount of a job search allowance shall be 90 percent of the total costs of each of the following...

  7. 20 CFR 617.34 - Amount.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... the Federal travel regulations (see 41 CFR part 101-7) for the locality where the job search is... WORKERS UNDER THE TRADE ACT OF 1974 Job Search Allowances § 617.34 Amount. (a) Computation. The amount of a job search allowance shall be 90 percent of the total costs of each of the following...

  8. 20 CFR 617.34 - Amount.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... the Federal travel regulations (see 41 CFR part 101-7) for the locality where the job search is... WORKERS UNDER THE TRADE ACT OF 1974 Job Search Allowances § 617.34 Amount. (a) Computation. The amount of a job search allowance shall be 90 percent of the total costs of each of the following...

  9. Estimation of Accumulation Parameters for Urban Runoff Quality Modeling

    NASA Astrophysics Data System (ADS)

    Alley, William M.; Smith, Peter E.

    1981-12-01

    Many recently developed watershed models utilize accumulation and washoff equations to simulate the quality of runofffrom urban impervious areas. These models often have been calibrated by trial and error and with little understanding of model sensitivity to the various parameters. Methodologies for estimating best fit values of the washoff parameters commonly used in these models have been presented previously. In this paper, parameter identification techniques for estimating the accumulation parameters from measured runoff quality data are presented along with a sensitivity analysis of the parameters. Results from application of the techniques and the sensitivity analysis suggest a need for data quantifying the magnitude and identifying the shape of constituent accumulation curves. An exponential accumulation curve is shown to be more general than the linear accumulation curves used in most urban runoff quality models. When determining accumulation rates, attention needs to be given to the effects of residual amounts of constituents remaining after the previous period of storm runoff or street sweeping.

  10. Joint enhancement of lead accumulation in Brassica plants by EDTA and ammonium sulfate in sand culture.

    PubMed

    Xiong, Zhi-ting; Lu, Ping

    2002-04-01

    When EDTA was added alone in the Pb-contaminated sand, the plant biomass and the total Pb amount in Plant decreased in both species, Brassica pekinensis and B. juncea var. multiceps, though the shoot Pb amount increased. In contrast, when (NH4)2SO4 was added alone in the Pb-contaminated sand, little effect was observed on the shoot Pb amount, though the root Pb amount was significantly increased in B. juncea var. multiceps. When amending EDTA and (NH4)2SO4 in combination, however, the shoot Pb amount in both species substantially increased, being, on an average, 2 times and 9 times higher than that in EDTA alone or (NH4)2SO4 alone amended treatment, respectively. The two amendments showed antagonism for plant growth, but synergism for Pb bioaccumulation. B. pekinensis showed its highest level of shoot and total Pb amount in the treatment amended with EDTA and (NH4)2SO4 only a half as much as in the other treatments. It is inferred that the mechanisms responsible for the joint-enhanced Pb accumulation might be concerned with the acidification of the growth medium, cation exchange reaction and relieving EDTA induced toxicity as results by amending ammonium sulfate. PMID:12046290

  11. 78 FR 59702 - Medicare Program; Medicare Appeals: Adjustment to the Amount in Controversy Threshold Amounts for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-27

    ...This notice announces the annual adjustment in the amount in controversy (AIC) threshold amounts for Administrative Law Judge (ALJ) hearings and judicial review under the Medicare appeals process. The adjustment to the AIC threshold amounts will be effective for requests for ALJ hearings and judicial review filed on or after January 1, 2014. The calendar year 2014 AIC threshold amounts are......

  12. Higher Education.

    ERIC Educational Resources Information Center

    Hendrickson, Robert M.; Gregory, Dennis E.

    Decisions made by federal and state courts during 1983 concerning higher education are reported in this chapter. Issues of employment and the treatment of students underlay the bulk of the litigation. Specific topics addressed in these and other cases included federal authority to enforce regulations against age discrimination and to revoke an…

  13. Higher Education.

    ERIC Educational Resources Information Center

    Hendrickson, Robert M.

    Litigation in 1987 was very brisk with an increase in the number of higher education cases reviewed. Cases discussed in this chapter are organized under four major topics: (1) intergovernmental relations; (2) employees, involving discrimination claims, tenured and nontenured faculty, collective bargaining and denial of employee benefits; (3)…

  14. Higher Education.

    ERIC Educational Resources Information Center

    Hendrickson, Robert M.; Finnegan, Dorothy E.

    The higher education case law in 1988 is extensive. Cases discussed in this chapter are organized under five major topics: (1) intergovernmental relations; (2) employees, involving discrimination claims, tenured and nontenured faculty, collective bargaining, and denial of employee benefits; (3) students, involving admissions, financial aid, First…

  15. Higher Education.

    ERIC Educational Resources Information Center

    Hendrickson, Robert M.

    This eighth chapter of "The Yearbook of School Law, 1986" summarizes and analyzes over 330 state and federal court cases litigated in 1985 in which institutions of higher education were involved. Among the topics examined were relationships between postsecondary institutions and various governmental agencies; discrimination in the employment of…

  16. Accumulation of arsenic in Lemna gibba L. (duckweed) in tailing waters of two abandoned uranium mining sites in Saxony, Germany.

    PubMed

    Mkandawire, Martin; Dudel, E Gert

    2005-01-01

    Accumulation of arsenic in Lemna gibba L. was investigated in tailing waters of abandoned uranium mine sites, following the hypothesis that arsenic poses contamination risks in post uranium mining in Saxony, Germany. Consequently, macrophytes growing in mine tailing waters accumulate high amounts of arsenic, which might be advantageous for biomonitoring arsenic transfer to higher trophic levels, and for phytoremediation. Water and L. gibba sample collected from pond on tailing dumps of abandoned mine sites at Lengenfeld and Neuensalz-Mechelgrun were analysed for arsenic. Laboratory cultures in nutrient solutions modified with six arsenic and three PO(4)(3-) concentrations were conducted to gain insight into the arsenic-L. gibba interaction. Arsenic accumulation coefficients in L. gibba were 10 times as much as the background concentrations in both tailing waters and nutrient solutions. Arsenic accumulations in L. gibba increased with arsenic concentration in the milieu but they decreased with phosphorus concentration. Significant reductions in arsenic accumulation in L. gibba were observed with the addition of PO(4)(3-) at all six arsenic test concentrations in laboratory experiments. Plant samples from laboratory trials had on average twofold higher bioaccumulation coefficients than tailing water at similar arsenic concentrations. This would be attributed to strong interaction among chemical components, and competition among ions in natural aquatic environment. The results of the study indicate that L. gibba can be a preliminary bioindicator for arsenic transfer from substrate to plants and might be used to monitor the transfer of arsenic from lower to higher trophic levels in the abandoned mine sites. There is also the potential of using L. gibba L. for arsenic phytoremediation of mine tailing waters because of its high accumulation capacity as demonstrated in this study. Transfer of arsenic contamination transported by accumulations in L. gibba carried with

  17. Trends and drivers of debris accumulation on Maui shorelines: Implications for local mitigation strategies.

    PubMed

    Blickley, Lauren C; Currie, Jens J; Kaufman, Gregory D

    2016-04-15

    Marine debris, particularly plastic, is an identified concern for coastal areas and is known to accumulate in large quantities in the North Pacific. Here we present results from the first study to quantify and compare the types and amounts of marine debris on Maui shorelines. Surveys were conducted monthly between May 2013 and December 2014, with additional daily surveys conducted on Maui's north shore during January 2015. Debris accumulation rates, loads, and sources varied between sites, with plastics being the most prevalent type of debris at all sites. Large debris loads on windward shores were attributed to the influence of the North Pacific Subtropical Gyre and northerly trade winds. Daily surveys resulted in a significantly higher rate of debris deposition than monthly surveys. The efficacy of local policy in debris mitigation showed promise, but was dependent upon the level of enforcement and consumer responsibility. PMID:26926778

  18. Accumulation of mercury and methylmercury by mushrooms and earthworms from forest soils.

    PubMed

    Rieder, Stephan R; Brunner, Ivano; Horvat, Milena; Jacobs, Anna; Frey, Beat

    2011-10-01

    Accumulation of total and methyl-Hg by mushrooms and earthworms was studied in thirty-four natural forest soils strongly varying in soil physico-chemical characteristics. Tissue Hg concentrations of both receptors did hardly correlate with Hg concentrations in soil. Both total and methyl-Hg concentrations in tissues were species-specific and dependent on the ecological groups of receptor. Methyl-Hg was low accounting for less than 5 and 8% of total Hg in tissues of mushrooms and earthworms, respectively, but with four times higher concentrations in earthworms than mushrooms. Total Hg concentrations in mushrooms averaged 0.96 mg Hg kg(-1) dw whereas litter decomposing mushrooms showed highest total Hg and methyl-Hg concentrations. Earthworms contained similar Hg concentrations (1.04 mg Hg kg(-1) dw) whereas endogeic earthworms accumulated highest amounts of Hg and methyl-Hg. PMID:21621314

  19. Intracellular accumulation of azithromycin by cultured human fibroblasts.

    PubMed Central

    Gladue, R P; Snider, M E

    1990-01-01

    Azithromycin was shown to achieve high concentrations in human skin fibroblasts. Intracellular penetration occurred rapidly (10 micrograms/mg of cellular protein after 3 h) and then increased progressively over a 3-day period; azithromycin accumulated up to 21 times more than erythromycin (61.1 versus 2.9 micrograms/mg of protein). Uptake was dependent on the extracellular concentration, was inhibited at 4 degrees C, did not occur in nonviable cells, and was reduced by a low pH. Intracellular accumulation was not affected by the metabolic inhibitor 2,4-dinitrophenol or sodium fluoride or by the nucleoside transport inhibitor 2-chloradenosine. Once concentrated in cells, azithromycin remained intracellular and was released slowly in the absence of extracellular drug, compared with erythromycin (17 versus 78% released after 1 h). After 48 h of incubation in drug-free medium, 27% of the initial amount of azithromycin remained cell associated. The release of azithromycin was not affected by various monokines reported to stimulate fibroblasts (interleukin-1 or tumor necrosis factor) or by exposure to bacteria. Incubation of azithromycin-loaded fibroblasts with human polymorphonuclear leukocytes resulted in a higher intracellular accumulation of azithromycin in polymorphonuclear leukocytes than in cells incubated with free nonintracellular azithromycin for the same time (8.3 versus 2.2 micrograms/ml after 2 h), suggesting a more efficient or rapid uptake through cell-to-cell interaction. The widespread distribution of fibroblasts in tissues suggests a potential for these cells, and possibly other lysosome-containing tissue cells, to serve as a reservoir for azithromycin, slowly releasing it for activity against extracellular organisms at sites of infection and passing it to phagocytes for activity against intracellular pathogens and potential transport to sites of infection. PMID:2168141

  20. Expression of cadR Enhances its Specific Activity for Cd Detoxification and Accumulation in Arabidopsis.

    PubMed

    Li, Jingrui; Wei, Xuezhi; Yu, Pengli; Deng, Xin; Xu, Wenxiu; Ma, Mi; Zhang, Haiyan

    2016-08-01

    Cadmium (Cd) is a transition metal that is highly toxic in biological systems. Anthropogenic emissions of Cd have increased biogeochemical cycling and the amount of Cd in the biosphere. Here we studied the utility of a bacterial Cd-binding protein, CadR, for the remediation of Cd contamination. CadR was successfully targeted to chloroplasts using a constitutive Cauliflower mosaic virus (CaMV) 35S promoter or a shoot-specific Chl a/b-binding protein 2 gene (CAB2) promoter and an RbcS (small subunit of the Rubisco complex) transit peptide. Under short-term (2 d) exposure to Cd, the cadR transgenic plants showed up to a 2.9-fold Cd accumulation in roots compared with untransformed plants. Under medium term (7 d) exposure to Cd, the concentrations of Cd in leaves began to increase but there were no differences between the wild type and the cadR transgenic plants. Under long-term (16 d) exposure to Cd, the cadR transgenic plants accumulated greater amounts of Cd in leaves than the untransformed plants. Total Cd accumulation (µg per plant) in shoots and roots of the plants expressing cadR were significantly higher (up to 3.5-fold in shoots and 5.2-fold in roots) than those of the untransformed plants. We also found that targeting CadR to chloroplasts facilitated chloroplastic metal homeostasis and Chl b accumulation. Our results demonstrate that manipulating chelating capacity in chloroplasts or in the cytoplasm may be effective in modifying both the accumulation of and resistance to Cd. PMID:27382127

  1. Bicarbonate trigger for inducing lipid accumulation in algal systems

    SciTech Connect

    Gardner, Robert; Peyton, Brent; Cooksey, Keith E.

    2015-08-04

    The present invention provides bicarbonate containing and/or bicarbonate-producing compositions and methods to induce lipid accumulation in an algae growth system, wherein the algae growth system is under light-dark cycling condition. By adding said compositions at a specific growth stage, said methods lead to much higher lipid accumulation and/or significantly reduced total time required for accumulating lipid in the algae growth system.

  2. 7 CFR 1424.8 - Payment amounts.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS BIOENERGY PROGRAM § 1424.8 Payment amounts. (a) An eligible... whose annual bioenergy production is: (i) Less than 65 million gallons, by dividing by 2.5; (ii)...

  3. 7 CFR 1424.8 - Payment amounts.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS BIOENERGY PROGRAM § 1424.8 Payment amounts. (a) An eligible... whose annual bioenergy production is: (i) Less than 65 million gallons, by dividing by 2.5; (ii)...

  4. 7 CFR 1424.8 - Payment amounts.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS BIOENERGY PROGRAM § 1424.8 Payment amounts. (a) An eligible... whose annual bioenergy production is: (i) Less than 65 million gallons, by dividing by 2.5; (ii)...

  5. 7 CFR 1424.8 - Payment amounts.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS BIOENERGY PROGRAM § 1424.8 Payment amounts. (a) An eligible... whose annual bioenergy production is: (i) Less than 65 million gallons, by dividing by 2.5; (ii)...

  6. 7 CFR 1424.8 - Payment amounts.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS BIOENERGY PROGRAM § 1424.8 Payment amounts. (a) An eligible... whose annual bioenergy production is: (i) Less than 65 million gallons, by dividing by 2.5; (ii)...

  7. Solids Accumulation Scouting Studies

    SciTech Connect

    Duignan, M. R.; Steeper, T. J.; Steimke, J. L.

    2012-09-26

    The objective of Solids Accumulation activities was to perform scaled testing to understand the behavior of remaining solids in a Double Shell Tank (DST), specifically AW-105, at Hanford during multiple fill, mix, and transfer operations. It is important to know if fissionable materials can concentrate when waste is transferred from staging tanks prior to feeding waste treatment plants. Specifically, there is a concern that large, dense particles containing plutonium could accumulate in poorly mixed regions of a blend tank heel for tanks that employ mixing jet pumps. At the request of the DOE Hanford Tank Operations Contractor, Washington River Protection Solutions, the Engineering Development Laboratory of the Savannah River National Laboratory performed a scouting study in a 1/22-scale model of a waste staging tank to investigate this concern and to develop measurement techniques that could be applied in a more extensive study at a larger scale. Simulated waste tank solids: Gibbsite, Zirconia, Sand, and Stainless Steel, with stainless steel particles representing the heavier particles, e.g., plutonium, and supernatant were charged to the test tank and rotating liquid jets were used to mix most of the solids while the simulant was pumped out. Subsequently, the volume and shape of the mounds of residual solids and the spatial concentration profiles for the surrogate for heavier particles were measured. Several techniques were developed and equipment designed to accomplish the measurements needed and they included: 1. Magnetic particle separator to remove simulant stainless steel solids. A device was designed and built to capture these solids, which represent the heavier solids during a waste transfer from a staging tank. 2. Photographic equipment to determine the volume of the solids mounds. The mounds were photographed as they were exposed at different tank waste levels to develop a composite of topographical areas. 3. Laser rangefinders to determine the volume of

  8. Comparison between the Amount of Environmental Change and the Amount of Transcriptome Change

    PubMed Central

    Ogata, Norichika; Kozaki, Toshinori; Yokoyama, Takeshi; Hata, Tamako; Iwabuchi, Kikuo

    2015-01-01

    Cells must coordinate adjustments in genome expression to accommodate changes in their environment. We hypothesized that the amount of transcriptome change is proportional to the amount of environmental change. To capture the effects of environmental changes on the transcriptome, we compared transcriptome diversities (defined as the Shannon entropy of frequency distribution) of silkworm fat-body tissues cultured with several concentrations of phenobarbital. Although there was no proportional relationship, we did identify a drug concentration “tipping point” between 0.25 and 1.0 mM. Cells cultured in media containing lower drug concentrations than the tipping point showed uniformly high transcriptome diversities, while those cultured at higher drug concentrations than the tipping point showed uniformly low transcriptome diversities. The plasticity of transcriptome diversity was corroborated by cultivations of fat bodies in MGM-450 insect medium without phenobarbital and in 0.25 mM phenobarbital-supplemented MGM-450 insect medium after previous cultivation (cultivation for 80 hours in MGM-450 insect medium without phenobarbital, followed by cultivation for 10 hours in 1.0 mM phenobarbital-supplemented MGM-450 insect medium). Interestingly, the transcriptome diversities of cells cultured in media containing 0.25 mM phenobarbital after previous cultivation (cultivation for 80 hours in MGM-450 insect medium without phenobarbital, followed by cultivation for 10 hours in 1.0 mM phenobarbital-supplemented MGM-450 insect medium) were different from cells cultured in media containing 0.25 mM phenobarbital after previous cultivation (cultivation for 80 hours in MGM-450 insect medium without phenobarbital). This hysteretic phenomenon of transcriptome diversities indicates multi-stability of the genome expression system. Cellular memories were recorded in genome expression networks as in DNA/histone modifications. PMID:26657512

  9. Comparison between the Amount of Environmental Change and the Amount of Transcriptome Change.

    PubMed

    Ogata, Norichika; Kozaki, Toshinori; Yokoyama, Takeshi; Hata, Tamako; Iwabuchi, Kikuo

    2015-01-01

    Cells must coordinate adjustments in genome expression to accommodate changes in their environment. We hypothesized that the amount of transcriptome change is proportional to the amount of environmental change. To capture the effects of environmental changes on the transcriptome, we compared transcriptome diversities (defined as the Shannon entropy of frequency distribution) of silkworm fat-body tissues cultured with several concentrations of phenobarbital. Although there was no proportional relationship, we did identify a drug concentration "tipping point" between 0.25 and 1.0 mM. Cells cultured in media containing lower drug concentrations than the tipping point showed uniformly high transcriptome diversities, while those cultured at higher drug concentrations than the tipping point showed uniformly low transcriptome diversities. The plasticity of transcriptome diversity was corroborated by cultivations of fat bodies in MGM-450 insect medium without phenobarbital and in 0.25 mM phenobarbital-supplemented MGM-450 insect medium after previous cultivation (cultivation for 80 hours in MGM-450 insect medium without phenobarbital, followed by cultivation for 10 hours in 1.0 mM phenobarbital-supplemented MGM-450 insect medium). Interestingly, the transcriptome diversities of cells cultured in media containing 0.25 mM phenobarbital after previous cultivation (cultivation for 80 hours in MGM-450 insect medium without phenobarbital, followed by cultivation for 10 hours in 1.0 mM phenobarbital-supplemented MGM-450 insect medium) were different from cells cultured in media containing 0.25 mM phenobarbital after previous cultivation (cultivation for 80 hours in MGM-450 insect medium without phenobarbital). This hysteretic phenomenon of transcriptome diversities indicates multi-stability of the genome expression system. Cellular memories were recorded in genome expression networks as in DNA/histone modifications. PMID:26657512

  10. The comparison of bacteremia and amount of bleeding during septoplasty.

    PubMed

    Koc, Sema; Uysal, Ismail Onder; Uysal, Elif Bilge; Yenişehirli, Gülgün; Duygu, Fazilet

    2012-04-01

    The aim of this study was to investigate the relationship between the amount of bleeding and bacteremia during nasal septal surgery. Seventy-one patients undergoing septoplasty were enrolled in the present study. The amount of bleeding was measured and recorded for each patient. Preoperative and postoperative blood cultures were collected immediately after the induction of anesthesia and 20 min after the operation, respectively. While none of the blood cultures taken preoperatively were positive for any organism, the cultures obtained postoperatively were positive in 9 (12.7%) of 71 patients who underwent septoplasty, and bacteremia was more frequent among those with a greater amount of bleeding during the surgery. The results of this study suggest that although bacteremia had no clinical consequences for patients, patients with more bleeding have an increased risk of developing bacteremia which may cause complications in higher risk individuals. PMID:22037718

  11. Theoretical lessons for increasing algal biofuel: Evolution of oil accumulation to avert carbon starvation in microalgae.

    PubMed

    Akita, Tetsuya; Kamo, Masashi

    2015-09-01

    Microalgae-derived oil is considered as a feasible alternative to fossil-derived oil. To produce more algal biomass, both algal population size and oil accumulation in algae must be maximized. Most of the previous studies have concentrated on only one of these issues, and relatively little attention has been devoted to considering the tradeoff between them. In this paper, we first theoretically investigated evolutionary reasons for oil accumulation and then by coupling population and evolutionary dynamics, we searched for conditions that may provide better yields. Using our model, we assume that algae allocate assimilated carbon to growth, maintenance, and carbon accumulation as biofuel and that the amount of essential materials (carbon and nitrate) are strongly linked in fixed proportions. Such stoichiometrically explicit models showed that (i) algae with more oil show slower population growth; therefore, the use of such algae results in lower total yields of biofuel and (ii) oil accumulation in algae is caused by carbon and not nitrate starvation. The latter can be interpreted as a strategy for avoiding the risk of increased death rate by carbon starvation. Our model also showed that both strong carbon starvation and moderately limited nitrate will promote total biofuel production. Our results highlight considering the life-history traits for a higher total yields of biofuel, which leads to insight into both establishing a prolonged culture and collection of desired strains from a natural environment. PMID:26047852

  12. The Effects of the Capital Accumulation Ratio on Wealth

    ERIC Educational Resources Information Center

    Harness, Nathaniel J.; Finke, Michael S.; Chatterjee, Swarn

    2009-01-01

    The capital accumulation ratio (CAR) is commonly used in academic research as a measure of household portfolio quality. This study tested whether a higher initial CAR impacts change in wealth over a decade among households in the accumulation life cycle stage. Meeting the 25% CAR guideline resulted in a 28.1% increase in net worth between 1994 and…

  13. Carbon accumulation and sequestration of lakes in China during the Holocene.

    PubMed

    Wang, Mei; Chen, Huai; Yu, Zicheng; Wu, Jianghua; Zhu, Qiu'an; Peng, Changhui; Wang, Yanfen; Qin, Boqiang

    2015-12-01

    Understanding the responses of lake systems to past climate change and human activity is critical for assessing and predicting the fate of lake carbon (C) in the future. In this study, we synthesized records of the sediment accumulation from 82 lakes and of C sequestration from 58 lakes with direct organic C measurements throughout China. We also identified the controlling factors of the long-term sediment and C accumulation dynamics in these lakes during the past 12 ka (1 ka = 1000 cal yr BP). Our results indicated an overall increasing trend of sediment and C accumulation since 12 ka, with an accumulation peak in the last couple of millennia for lakes in China, corresponding to terrestrial organic matter input due to land-use change. The Holocene lake sediment accumulation rate (SAR) and C accumulation rate (CAR) averaged (mean ± SE) 0.47 ± 0.05 mm yr(-1) and 7.7 ± 1.4 g C m(-2)  yr(-1) in China, respectively, comparable to the previous estimates for boreal and temperate regions. The SAR for lakes in the East Plain of subtropical China (1.05 ± 0.28 mm yr(-1) ) was higher than those in other regions (P < 0.05). However, CAR did not vary significantly among regions. Overall, the variability and history of climate and anthropogenic interference regulated the temporal and spatial dynamics of sediment and C sequestration for lakes in China. We estimated the total amount of C burial in lakes of China as 8.0 ± 1.0 Pg C. This first estimation of total C storage and dynamics in lakes of China confirms the importance of lakes in land C budget in monsoon-influenced regions. PMID:26220607

  14. 76 FR 59138 - Medicare Program; Medicare Appeals; Adjustment to the Amount in Controversy Threshold Amounts for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-23

    ... HUMAN SERVICES Centers for Medicare & Medicaid Services Medicare Program; Medicare Appeals; Adjustment to the Amount in Controversy Threshold Amounts for Calendar Year 2012 AGENCY: Centers for Medicare... review under the Medicare appeals process. The adjustment to the AIC threshold amounts will be...

  15. Quantifying the amount and activity of Rubisco in leaves.

    PubMed

    Kubien, David S; Brown, Christopher M; Kane, Heather J

    2011-01-01

    The CO2-fixing enzyme Rubisco plays a crucial biological role as a primary determinant of both plant yield and the response of the biosphere to global change. Here, we describe techniques for measuring the amount and activity of Rubisco in higher plants. To accommodate a range of experimental capabilities, we describe basic radioisotopic methods as well as non-radioactive techniques. The required calculations are included. We discuss problems that commonly arise during the extraction and assay of Rubisco. PMID:20960142

  16. Atmospheric mercury accumulation and washoff processes on impervious urban surfaces

    USGS Publications Warehouse

    Eckley, C.S.; Branfireun, B.; Diamond, M.; Van Metre, P.C.; Heitmuller, F.

    2008-01-01

    The deposition and transport of mercury (Hg) has been studied extensively in rural environments but is less understood in urbanized catchments, where elevated atmospheric Hg concentrations and impervious surfaces may efficiently deliver Hg to waterways in stormwater runoff. We determined the rate at which atmospheric Hg accumulates on windows, identified the importance of washoff in removing accumulated Hg, and measured atmospheric Hg concentrations to help understand the relationship between deposition and surface accumulation. The main study location was Toronto, Ontario. Similar samples were also collected from Austin, Texas for comparison of Hg accumulation between cities. Windows provided a good sampling surface because they are ubiquitous in urban environments and are easy to clean/blank allowing the assessment of contemporary Hg accumulation. Hg Accumulation rates were spatially variable ranging from 0.82 to 2.7 ng m-2 d-1 in Toronto and showed similar variability in Austin. The highest accumulation rate in Toronto was at the city center and was 5?? higher than the rural comparison site (0.58 ng m-2 d-1). The atmospheric total gaseous mercury (TGM) concentrations were less than 2?? higher between the rural and urban locations (1.7 ?? 0.3 and 2.7 ?? 1.1 ng m-3, respectively). The atmospheric particulate bound fraction (HgP), however, was more than 3?? higher between the rural and urban sites, which may have contributed to the higher urban Hg accumulation rates. Windows exposed to precipitation had 73 ?? 9% lower accumulation rates than windows sheltered from precipitation. Runoff collected from simulated rain events confirmed that most Hg accumulated on windows was easily removed and that most of the Hg in washoff was HgP. Our results indicate that the Hg flux from urban catchments will respond rapidly to changes in atmospheric concentrations due to the mobilization of the majority of the surface accumulated Hg during precipitation events. ?? 2008 Elsevier

  17. Guidelines for Waste Accumulation Areas (WAAs) at LBL. Revision 1

    SciTech Connect

    Not Available

    1994-06-01

    The purpose of this document is to set conditions for establishing and containing areas for the accumulation of hazardous waste at LBL. Areas designed for accumulation of these wastes for up to 90 days in quantities greater than 55 gallons (208 liters) of hazardous waste, one quart (0.946 liter) of extremely hazardous waste, or one quart (0.946 liter) of acutely hazardous waste are called Waste Accumulation Areas (WAAs). Areas designed for accumulation of wastes in smaller amounts are called Satellite Accumulation Areas (SAAs). This document provides guidelines for employee and organizational responsibilities for WAAs, constructing a WAA, storing waste in a WAA, operating and maintaining a WAA, and responding to spills in a WAA.

  18. ITER helium ash accumulation

    SciTech Connect

    Hogan, J.T.; Hillis, D.L.; Galambos, J.; Uckan, N.A. ); Dippel, K.H.; Finken, K.H. . Inst. fuer Plasmaphysik); Hulse, R.A.; Budny, R.V. . Plasma Physics Lab.)

    1990-01-01

    Many studies have shown the importance of the ratio {upsilon}{sub He}/{upsilon}{sub E} in determining the level of He ash accumulation in future reactor systems. Results of the first tokamak He removal experiments have been analysed, and a first estimate of the ratio {upsilon}{sub He}/{upsilon}{sub E} to be expected for future reactor systems has been made. The experiments were carried out for neutral beam heated plasmas in the TEXTOR tokamak, at KFA/Julich. Helium was injected both as a short puff and continuously, and subsequently extracted with the Advanced Limiter Test-II pump limiter. The rate at which the He density decays has been determined with absolutely calibrated charge exchange spectroscopy, and compared with theoretical models, using the Multiple Impurity Species Transport (MIST) code. An analysis of energy confinement has been made with PPPL TRANSP code, to distinguish beam from thermal confinement, especially for low density cases. The ALT-II pump limiter system is found to exhaust the He with maximum exhaust efficiency (8 pumps) of {approximately}8%. We find 1<{upsilon}{sub He}/{upsilon}{sub E}<3.3 for the database of cases analysed to date. Analysis with the ITER TETRA systems code shows that these values would be adequate to achieve the required He concentration with the present ITER divertor He extraction system.

  19. Supply of fatty acid is one limiting factor in the accumulation of triacylglycerol in developing embryos

    SciTech Connect

    Bao, X.; Ohlrogge, J.

    1999-08-01

    The metabolic factors that determine oil yield in seeds are still not well understood. To begin to examine the limits on triacylglycerol (TAG) production, developing Cuphea lanceolata, Ulmus carpinifolia, and Ulmus parvifolia embryos were incubated with factors whose availability might limit oil accumulation. The addition of glycerol or sucrose did not significantly influence the rate of TAG synthesis. However, the rate of {sup 14}C-TAG synthesis upon addition of 2.1 mM {sup 14}C-decanoic acid (10:0) was approximately four times higher than the in vivo rate of TAG accumulation in C. lanceolata and two times higher than the in vivo rate in U. carpinifolia and U. parvifolia. In C. lanceolata embryos, the highest rate of {sup 14}C-TAG synthesis (14.3 nmol h{sup {minus}1} embryo {sup {minus}1}) was achieved with the addition of 3.6 mM decanoic acid. {sup 14}C-Decanoic acid was incorporated equally well in all three acyl positions of TAG. The results suggest that C. lancelata, U. Carpinifolia, and U. parvifolia embryos have sufficient acyltransferase activities and glycerol-3-phosphate levels to support rates of TAG synthesis in excess of those found in vivo. Consequently, the amount of TAG synthesized in these oilseeds may be in part determined by the amount of fatty acid produced in plastids.

  20. Stover Composition in Maize and Sorghum Reveals Remarkable Genetic Variation and Plasticity for Carbohydrate Accumulation

    PubMed Central

    Sekhon, Rajandeep S.; Breitzman, Matthew W.; Silva, Renato R.; Santoro, Nicholas; Rooney, William L.; de Leon, Natalia; Kaeppler, Shawn M.

    2016-01-01

    Carbohydrates stored in vegetative organs, particularly stems, of grasses are a very important source of energy. We examined carbohydrate accumulation in adult sorghum and maize hybrids with distinct phenology and different end uses (grain, silage, sucrose or sweetness in stalk juice, and biomass). Remarkable variation was observed for non-structural carbohydrates and structural polysaccharides during three key developmental stages both between and within hybrids developed for distinct end use in both species. At the onset of the reproductive phase (average 65 days after planting, DAP), a wide range for accumulation of non-structural carbohydrates (free glucose and sucrose combined), was observed in internodes of maize (11–24%) and sorghum (7–36%) indicating substantial variation for transient storage of excess photosynthate during periods of low grain or vegetative sink strength. Remobilization of these reserves for supporting grain fill or vegetative growth was evident from lower amounts in maize (8–19%) and sorghum (9–27%) near the end of the reproductive period (average 95 DAP). At physiological maturity of grain hybrids (average 120 DAP), amounts of these carbohydrates were generally unchanged in maize (9–21%) and sorghum (16–27%) suggesting a loss of photosynthetic assimilation due to weakening sink demand. Nonetheless, high amounts of non-structural carbohydrates at maturity even in grain maize and sorghum (15–18%) highlight the potential for developing dual-purpose (grain/stover) crops. For both species, the amounts of structural polysaccharides in the cell wall, measured as monomeric components (glucose and pentose), decreased during grain fill but remained unchanged thereafter with maize biomass possessing slightly higher amounts than sorghum. Availability of carbohydrates in maize and sorghum highlights the potential for developing energy-rich dedicated biofuel or dual-purpose (grain/stover) crops. PMID:27375668

  1. Stover composition in maize and sorghum reveals remarkable genetic variation and plasticity for carbohydrate accumulation

    DOE PAGESBeta

    Sekhon, Rajandeep S.; Breitzman, Matthew W.; Silva, Renato R.; Santoro, Nicholas; Rooney, William L.; de Leon, Natalia; Kaeppler, Shawn M.

    2016-06-08

    Carbohydrates stored in vegetative organs, particularly stems, of grasses are a very important source of energy. We examined carbohydrate accumulation in adult sorghum and maize hybrids with distinct phenology and different end uses (grain, silage, sucrose or sweetness in stalk juice, and biomass). Remarkable variation was observed for nonstructural carbohydrates and structural polysaccharides during three key developmental stages both between and within hybrids developed for distinct end use in both species. At the onset of the reproductive phase (average 65 days after planting, DAP), a wide range for accumulation of non-structural carbohydrates (free glucose and sucrose combined), was observed inmore » internodes of maize (11-24%) and sorghum (7-36%) indicating substantial variation for transient storage of excess photosynthate during periods of low grain or vegetative sink strength. Remobilization of these reserves for supporting grain fill or vegetative growth was evident from lower amounts in maize (8-19%) and sorghum (9-27%) near the end of the reproductive period (average 95 DAP). At physiological maturity of grain hybrids (average 120 DAP), amounts of these carbohydrates were generally unchanged in maize (9-21%) and sorghum (16-27%) suggesting a loss of photosynthetic assimilation due to weakening sink demand. Nonetheless, high amounts of non-structural carbohydrates at maturity even in grain maize and sorghum (15-18%) highlight the potential for developing dual-purpose (grain/stover) crops. For both species, the amounts of structural polysaccharides in the cell wall, measured as monomeric components (glucose and pentose), decreased during grain fill but remained unchanged thereafter with maize biomass possessing slightly higher amounts than sorghum. In conclusion, availability of carbohydrates in maize and sorghum highlights the potential for developing energy-rich dedicated biofuel or dual-purpose (grain/stover) crops.« less

  2. Stover Composition in Maize and Sorghum Reveals Remarkable Genetic Variation and Plasticity for Carbohydrate Accumulation.

    PubMed

    Sekhon, Rajandeep S; Breitzman, Matthew W; Silva, Renato R; Santoro, Nicholas; Rooney, William L; de Leon, Natalia; Kaeppler, Shawn M

    2016-01-01

    Carbohydrates stored in vegetative organs, particularly stems, of grasses are a very important source of energy. We examined carbohydrate accumulation in adult sorghum and maize hybrids with distinct phenology and different end uses (grain, silage, sucrose or sweetness in stalk juice, and biomass). Remarkable variation was observed for non-structural carbohydrates and structural polysaccharides during three key developmental stages both between and within hybrids developed for distinct end use in both species. At the onset of the reproductive phase (average 65 days after planting, DAP), a wide range for accumulation of non-structural carbohydrates (free glucose and sucrose combined), was observed in internodes of maize (11-24%) and sorghum (7-36%) indicating substantial variation for transient storage of excess photosynthate during periods of low grain or vegetative sink strength. Remobilization of these reserves for supporting grain fill or vegetative growth was evident from lower amounts in maize (8-19%) and sorghum (9-27%) near the end of the reproductive period (average 95 DAP). At physiological maturity of grain hybrids (average 120 DAP), amounts of these carbohydrates were generally unchanged in maize (9-21%) and sorghum (16-27%) suggesting a loss of photosynthetic assimilation due to weakening sink demand. Nonetheless, high amounts of non-structural carbohydrates at maturity even in grain maize and sorghum (15-18%) highlight the potential for developing dual-purpose (grain/stover) crops. For both species, the amounts of structural polysaccharides in the cell wall, measured as monomeric components (glucose and pentose), decreased during grain fill but remained unchanged thereafter with maize biomass possessing slightly higher amounts than sorghum. Availability of carbohydrates in maize and sorghum highlights the potential for developing energy-rich dedicated biofuel or dual-purpose (grain/stover) crops. PMID:27375668

  3. 7 CFR 1421.304 - Payment amount.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 2012 Crop of Wheat, Barley, Oats, and Triticale § 1421.304 Payment amount. (a) The grazing payment rate... payment rate in effect for the predominant class of wheat in the county where the farm is located as of... three (3) similar farms. For triticale, the payment yield shall be the yield for wheat from three...

  4. 7 CFR 1421.304 - Payment amount.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 2012 Crop of Wheat, Barley, Oats, and Triticale § 1421.304 Payment amount. (a) The grazing payment rate... payment rate in effect for the predominant class of wheat in the county where the farm is located as of... three (3) similar farms. For triticale, the payment yield shall be the yield for wheat from three...

  5. 7 CFR 1421.304 - Payment amount.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 2012 Crop of Wheat, Barley, Oats, and Triticale § 1421.304 Payment amount. (a) The grazing payment rate... payment rate in effect for the predominant class of wheat in the county where the farm is located as of... three (3) similar farms. For triticale, the payment yield shall be the yield for wheat from three...

  6. 24 CFR 201.10 - Loan amounts.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Loan amounts. 201.10 Section 201.10 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT MORTGAGE AND LOAN INSURANCE...

  7. 7 CFR 1421.304 - Payment amount.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 2012 Crop of Wheat, Barley, Oats, and Triticale § 1421.304 Payment amount. (a) The grazing payment rate... complete program application to CCC. For triticale, the grazing rate will be equal to the loan deficiency... three (3) similar farms. For triticale, the payment yield shall be the yield for wheat from three...

  8. 14 CFR 1300.13 - Guarantee amount.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Guarantee amount. 1300.13 Section 1300.13 Aeronautics and Space AIR TRANSPORTATION SYSTEM STABILIZATION OFFICE OF MANAGEMENT AND BUDGET AVIATION DISASTER RELIEF-AIR CARRIER GUARANTEE LOAN PROGRAM Minimum Requirements and Application Procedures §...

  9. 14 CFR 1300.13 - Guarantee amount.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Guarantee amount. 1300.13 Section 1300.13 Aeronautics and Space AIR TRANSPORTATION SYSTEM STABILIZATION OFFICE OF MANAGEMENT AND BUDGET AVIATION DISASTER RELIEF-AIR CARRIER GUARANTEE LOAN PROGRAM Minimum Requirements and Application Procedures §...

  10. 20 CFR 340.2 - Amount recoverable.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... amount of unemployment, sickness, or maternity benefits paid under the Railroad Unemployment Insurance... been determined to be days of unemployment or sickness; (c) Recoverable under section 4(a-1)(ii) of the..., unemployment, sickness or maternity benefits under any law other than the Railroad Unemployment Insurance...

  11. 20 CFR 340.2 - Amount recoverable.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... amount of unemployment, sickness, or maternity benefits paid under the Railroad Unemployment Insurance... been determined to be days of unemployment or sickness; (c) Recoverable under section 4(a-1)(ii) of the..., unemployment, sickness or maternity benefits under any law other than the Railroad Unemployment Insurance...

  12. 20 CFR 340.2 - Amount recoverable.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... amount of unemployment, sickness, or maternity benefits paid under the Railroad Unemployment Insurance... been determined to be days of unemployment or sickness; (c) Recoverable under section 4(a-1)(ii) of the..., unemployment, sickness or maternity benefits under any law other than the Railroad Unemployment Insurance...

  13. 20 CFR 340.2 - Amount recoverable.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... amount of unemployment, sickness, or maternity benefits paid under the Railroad Unemployment Insurance... been determined to be days of unemployment or sickness; (c) Recoverable under section 4(a-1)(ii) of the..., unemployment, sickness or maternity benefits under any law other than the Railroad Unemployment Insurance...

  14. 20 CFR 340.2 - Amount recoverable.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... amount of unemployment, sickness, or maternity benefits paid under the Railroad Unemployment Insurance... been determined to be days of unemployment or sickness; (c) Recoverable under section 4(a-1)(ii) of the..., unemployment, sickness or maternity benefits under any law other than the Railroad Unemployment Insurance...

  15. 33 CFR 133.7 - Requests: Amount.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... POLLUTION FINANCIAL RESPONSIBILITY AND COMPENSATION OIL SPILL LIABILITY TRUST FUND; STATE ACCESS § 133.7... amount anticipated for immediate removal action for a single oil pollution incident, but, in any event... quantity and composition of the oil, weather conditions and customary costs of similar services in...

  16. 33 CFR 133.7 - Requests: Amount.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... POLLUTION FINANCIAL RESPONSIBILITY AND COMPENSATION OIL SPILL LIABILITY TRUST FUND; STATE ACCESS § 133.7... amount anticipated for immediate removal action for a single oil pollution incident, but, in any event... quantity and composition of the oil, weather conditions and customary costs of similar services in...

  17. 33 CFR 133.7 - Requests: Amount.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... POLLUTION FINANCIAL RESPONSIBILITY AND COMPENSATION OIL SPILL LIABILITY TRUST FUND; STATE ACCESS § 133.7... amount anticipated for immediate removal action for a single oil pollution incident, but, in any event... quantity and composition of the oil, weather conditions and customary costs of similar services in...

  18. 33 CFR 133.7 - Requests: Amount.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... POLLUTION FINANCIAL RESPONSIBILITY AND COMPENSATION OIL SPILL LIABILITY TRUST FUND; STATE ACCESS § 133.7... amount anticipated for immediate removal action for a single oil pollution incident, but, in any event... quantity and composition of the oil, weather conditions and customary costs of similar services in...

  19. 33 CFR 133.7 - Requests: Amount.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... POLLUTION FINANCIAL RESPONSIBILITY AND COMPENSATION OIL SPILL LIABILITY TRUST FUND; STATE ACCESS § 133.7... amount anticipated for immediate removal action for a single oil pollution incident, but, in any event... quantity and composition of the oil, weather conditions and customary costs of similar services in...

  20. 7 CFR 1421.304 - Payment amount.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 2012 Crop of Wheat, Barley, Oats, and Triticale § 1421.304 Payment amount. (a) The grazing payment rate... payment rate in effect for the predominant class of wheat in the county where the farm is located as of... three (3) similar farms. For triticale, the payment yield shall be the yield for wheat from three...

  1. 40 CFR 35.9050 - Assistance amount.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Assistance amount. 35.9050 Section 35.9050 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Financial Assistance for the National Estuary Program § 35.9050...

  2. 40 CFR 35.9050 - Assistance amount.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Assistance amount. 35.9050 Section 35.9050 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Financial Assistance for the National Estuary Program § 35.9050...

  3. 40 CFR 35.9050 - Assistance amount.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Assistance amount. 35.9050 Section 35.9050 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Financial Assistance for the National Estuary Program § 35.9050...

  4. 40 CFR 35.9050 - Assistance amount.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Assistance amount. 35.9050 Section 35.9050 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Financial Assistance for the National Estuary Program § 35.9050...

  5. 40 CFR 35.9050 - Assistance amount.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Assistance amount. 35.9050 Section 35.9050 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Financial Assistance for the National Estuary Program § 35.9050...

  6. 27 CFR 70.243 - Exempt amount.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2014-04-01 2014-04-01 false Exempt amount. 70.243 Section 70.243 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... as wages, salary, or other income for each payroll period described in § 70.244 of this part...

  7. 27 CFR 70.243 - Exempt amount.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Exempt amount. 70.243 Section 70.243 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... as wages, salary, or other income for each payroll period described in § 70.244 of this part...

  8. 27 CFR 70.243 - Exempt amount.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2011-04-01 2011-04-01 false Exempt amount. 70.243 Section 70.243 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... as wages, salary, or other income for each payroll period described in § 70.244 of this part...

  9. 27 CFR 70.243 - Exempt amount.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2013-04-01 2013-04-01 false Exempt amount. 70.243 Section 70.243 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... as wages, salary, or other income for each payroll period described in § 70.244 of this part...

  10. 27 CFR 70.243 - Exempt amount.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2012-04-01 2011-04-01 true Exempt amount. 70.243 Section 70.243 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... as wages, salary, or other income for each payroll period described in § 70.244 of this part...

  11. Assessment of storage lipid accumulation patterns in eucalanoid copepods from the eastern tropical Pacific Ocean

    NASA Astrophysics Data System (ADS)

    Cass, Christine J.; Daly, Kendra L.; Wakeham, Stuart G.

    2014-11-01

    Members of the copepod family Eucalanidae are widely distributed throughout the world's oceans and have been noted for their accumulation of storage lipids in high- and low-latitude environments. However, little is known about the lipid composition of eucalanoid copepods in low-latitude environments. The purpose of this study was to examine fatty acid and alcohol profiles in the storage lipids (wax esters and triacylglycerols) of Eucalanus inermis, Rhincalanus rostrifrons, R. nasutus, Pareucalanus attenuatus, and Subeucalanus subtenuis, collected primarily in the eastern tropical north Pacific near the Tehuantepec Bowl and Costa Rica Dome regions, noted for its oxygen minimum zone, during fall 2007 and winter 2008/2009. Adult copepods and particulate material were collected in the upper 50 m and from 200 to 300 m in the upper oxycline. Lipid profiles of particulate matter were generated to help ascertain information on ecological strategies of these species and on differential accumulation of dietary and modified fatty acids in the wax ester and triacylglycerol storage lipid components of these copepods in relation to their vertical distributions around the oxygen minimum zone. Additional data on phospholipid fatty acid and sterol/fatty alcohol fractions were also generated to obtain a comprehensive lipid data set for each sample. Rhincalanus spp. accumulated relatively large amounts of storage lipids (31-80% of dry mass (DM)), while E. inermis had moderate amounts (2-9% DM), and P. attenuatus and S. subtenuis had low quantities of storage lipid (0-1% DM). E. inermis and S. subtenuis primarily accumulated triacylglycerols (>90% of storage lipids), while P. attenuatus and Rhincalanus spp. primarily accumulated wax esters (>84% of storage lipids). Based on previously generated molecular phylogenies of the Eucalanidae family, these results appear to support genetic predisposition as a major factor explaining why a given species accumulates primarily triacylglycerols

  12. Molecular cloning and characterization of tyrosine aminotransferase and hydroxyphenylpyruvate reductase, and rosmarinic acid accumulation in Scutellaria baicalensis.

    PubMed

    Kim, Yeon Bok; Uddina, Md Romij; Kim, YeJi; Park, Chun Geon; Park, Sang Un

    2014-09-01

    Rosmarinic acid (a-O-caffeoyl-3,4-dihydroxyphenylacetic acid, RA) is a caffeoyl ester widely distributed in plants. cDNA clones encoding tyrosine aminotransferase (TAT1 and 2) and hydroxyphenylpyruvate reductase (HPPR) have been isolated from Scutellaria baicalensis. The open reading frames (ORFs) of SbTAT1 and 2 were 1230 and 1272 bp long and encoded 409 and 423 amino acid residues, respectively. HPPR corresponded to a 942-bp ORF and 313 amino acid residues of translated protein. To study the molecular mechanisms of TAT and HPPR and investigate RA accumulation in S. baicalensis, we examined the transcript levels of TAT isoforms and HPPR with quantitative real-time PCR and analyzed the RA content in different organs by using high-performance liquid chromatography. The transcript levels of SbTATI SbTAT2, and SbHPPR in the flowers were higher than those in other organs. RA was also highly accumulated in the flowers and with a trace amount in the roots. No RA was detected in the leaves and stems of S. baicalensis. The amount of accumulated RA in the flowers was 28.7 times higher than that in the roots. Our results will be helpful in elucidating the mechanisms of RA biosynthesis in S. baicalensis. PMID:25918800

  13. Do radial oxygen loss and external aeration affect iron plaque formation and arsenic accumulation and speciation in rice?

    PubMed Central

    Wu, Chuan; Ye, Zhihong; Li, Hui; Wu, Shengchun; Deng, Dan; Zhu, Yongguan; Wong, Minghung

    2012-01-01

    Hydroponic experiments were conducted to investigate the effect of radial oxygen loss (ROL) and external aeration on iron (Fe) plaque formation, and arsenic (As) accumulation and speciation in rice (Oryza sativa L.). The data showed that there were significant correlations between ROL and Fe concentrations in Fe plaque produced on different genotypes of rice. There were also significant differences in the amounts of Fe plaque formed between different genotypes in different positions of roots and under different aeration conditions (aerated, normal, and stagnant treatments). In aerated treatments, rice tended to have a higher Fe plaque formation than in a stagnant solution, with the greatest formation at the root tip decreasing with increasing distances away, in accordance with a trend of spatial ROL. Genotypes with higher rates of ROL induced higher degrees of Fe plaque formation. Plaques sequestered As on rice roots, with arsenate almost double that with arsenite, leading to decreased As accumulation in both roots and shoots. The major As species detected in roots and shoots was arsenite, ranging from 34 to 78% of the total As in the different treatments and genotypes. These results contribute to our understanding of genotypic differences in As uptake by rice and the mechanisms causing rice genotypes with higher ROL to show lower overall As accumulation. PMID:22345639

  14. Enantioselective accumulation of chiral polychlorinated biphenyls in lotus plant (Nelumbonucifera spp.).

    PubMed

    Dai, Shouhui; Wong, Charles S; Qiu, Jing; Wang, Min; Chai, Tingting; Fan, Li; Yang, Shuming

    2014-09-15

    Enantioselective accumulation of chiral polychlorinated biphenyls (PCBs) 91, 95, 136, 149, 176 and 183 was investigated in lotus plants (Nelumbonucifera spp.) exposed to these chemicals via spiked sediment, to determine uptake and possible biotransformation for aquatic phytoremediation purposes. The concentrations of most PCBs were greatest in roots at 60 d (19.6 ± 1.51-70.6 ± 6.14 μg kg(-1)), but were greatest in stems and leaves at 120 d (25.3 ± 6.14-95.5 ± 19.4 μg kg(-1) and 17.4 ± 4.41-70.4 ± 10.4 μg kg(-1), respectively). Total amounts were greatest at 120 d and significantly higher in roots than those in stems and in leaves (1,457 ± 220-5,852 ± 735 ng, 237 ± 47.1-902 ± 184 ng and 202 ± 60.3-802 ± 90.2 ng, respectively), but represented less than 0.51% of the total mass of PCBs added to sediments, indicating that lotus plants were unlikely to remove appreciable amounts of PCBs from contaminated sediments. Racemic PCB residues in sediment indicate no enantioselective biodegradation by sedimentary microbial consortia over the entire experiment. Preferential accumulation of the (-)-enantiomers of PCBs 91, 95 and 136 were observed in roots, stems and leaves, but non-enantioselective accumulation was observed for PCBs 149, 176 and 183. These results indicate that aquatic plants can accumulate PCBs enantioselectively via root uptake, possibly by biotransformation within plant tissues as observed for terrestrial plants. This is also the first report to identify optical rotation of the atropisomers of PCBs 91 and 95. PMID:25218260

  15. Amplification of trace amounts of nucleic acids

    DOEpatents

    Church, George M.; Zhang, Kun

    2008-06-17

    Methods of reducing background during amplification of small amounts of nucleic acids employ careful analysis of sources of low level contamination. Ultraviolet light can be used to reduce nucleic acid contaminants in reagents and equipment. "Primer-dimer" background can be reduced by judicious design of primers. We have shown clean signal-to-noise with as little as starting material as one single human cell (.about.6 picogram), E. coli cell (.about.5 femtogram) or Prochlorococcus cell (.about.3 femtogram).

  16. Measurement of intravenously administered γ-Fe2O3 particle amount in mice tissues using vibrating sample magnetometer.

    PubMed

    Kishimoto, Mikio; Miyamoto, Ryoichi; Oda, Tatsuya; Ohara, Yusuke; Yanagihara, Hideto; Ohkohchi, Nobuhiro; Kita, Eiji

    2014-12-01

    Dispersions of platelet γ-Fe2O3 particles 30-50nm in size were intravenously administered to mice and the amount of particles accumulated in each tissue was obtained by magnetization measurement using a vibrating sample magnetometer. Background noise was greatly reduced by measuring dried tissues under a magnetic field of 500 Oe so that the effect of diamagnetism was slight. Remarkable particle accumulation was observed in the liver and spleen. Considerable particle accumulation was observed in the lung when a large quantity of γ-Fe2 O3 particles was administered. There was no significant particle accumulation in the kidney and heart. PMID:25122839

  17. An engineered lipid remodeling system using a galactolipid synthase promoter during phosphate starvation enhances oil accumulation in plants

    PubMed Central

    Shimojima, Mie; Madoka, Yuka; Fujiwara, Ryota; Murakawa, Masato; Yoshitake, Yushi; Ikeda, Keiko; Koizumi, Ryota; Endo, Keiji; Ozaki, Katsuya; Ohta, Hiroyuki

    2015-01-01

    Inorganic phosphate (Pi) depletion is a serious problem for plant growth. Membrane lipid remodeling is a defense mechanism that plants use to survive Pi-depleted conditions. During Pi starvation, phospholipids are degraded to supply Pi for other essential biological processes, whereas galactolipid synthesis in plastids is up-regulated via the transcriptional activation of monogalactosyldiacylglycerol synthase 3 (MGD3). Thus, the produced galactolipids are transferred to extraplastidial membranes to substitute for phospholipids. We found that, Pi starvation induced oil accumulation in the vegetative tissues of various seed plants without activating the transcription of enzymes involved in the later steps of triacylglycerol (TAG) biosynthesis. Moreover, the Arabidopsis starchless phosphoglucomutase mutant, pgm-1, accumulated higher TAG levels than did wild-type plants under Pi-depleted conditions. We generated transgenic plants that expressed a key gene involved in TAG synthesis using the Pi deficiency–responsive MGD3 promoter in wild-type and pgm-1 backgrounds. During Pi starvation, the transgenic plants accumulated higher TAG amounts compared with the non-transgenic plants, suggesting that the Pi deficiency–responsive promoter of galactolipid synthase in plastids may be useful for producing transgenic plants that accumulate more oil under Pi-depleted conditions. PMID:26379690

  18. Elemental accumulation studied in biological species

    SciTech Connect

    Not Available

    1986-01-01

    At The Geysers, relatively little environmental baseline data were collected during the early years of development. In early 1983, the CEC awarded Sonoma County a geothermal grant to analyze the biological accumulation of trace elements in The Geysers Geothermal region. Prior studies in The Geysers region have established data for 27 different chemical elements, and suggest that chemicals are accumulating near power plants. This study examined selected species of rodents, fish, and lichen. Elevated amounts of chemical elements were found in their tissues. It is not clear if this accumulation is the result of geothermal development or due to naturally high backgrounds of these elements in the region. However, today these element loads serve as reference points for both developers and regulators. The CEC awarded a second grant in July 1985. The study funded by this grant will provide a more complete analysis of elemental loads by examining species such as western fence lizards and deer. Results and conclusions from these two studies can be used by regulatory agencies planning for future geothermal development in The Geysers region.

  19. Carbon accumulation by biological soil crusts in relation to relief and sampling depth

    NASA Astrophysics Data System (ADS)

    Jetter, Stefan; Drahorad, Sylvie; Felix-Henningsen, Peter

    2010-05-01

    In arid and semiarid ecosystems the soil surface is covered by biological soil crusts (BSC). These BSC are microbial communities of cyanobacteria, lichens and mosses. Due to the photosynthetic activity of these microorganisms, BSC are main carbon contributors to arid ecosystems. The cover is related to ecosystem functions like surface stabilization, water redistribution and nutrient fixation. These functions rely on the microbial community composition of the BSC. Cyanobacteria and cyanolichens excrete exopolysaccharides, which build microaggregates with soil particles. This stabilizes and seals the soil surface. Therefore cyanobacteria and cyanolichen dominated crusts introduce runoff, which affects the distribution of carbon. The total amount of soil organic carbon was determined in relation to the relief position and BSC thickness showing a strong correlation between relief, sampling depth and carbon amounts. At the Arid Ecosystem Research Center (AERC) station of the Nizzana sand dunes (NW Negev, Israel) the dunes and the interdune corridor are covered by BSC up to 80% of the total area. The BSC are composed of a thin topcrust section and a mineral subcrust section. The overall thickness changes in relation to the relief position. Along a dune transect topcrust and subcrust samples were taken and analyzed on their C_org, C_carb, and C_total concentration. The total amount of carbon (g m^-2) was calculated from the carbon concentrations, the BSC bulk density and the sampling depth. Comparing the topcrust and subcrust values of the sampling points the topcrust sections showed 3-4 times higher concentrations of organic carbon than the subcrust sections. The light intensity decreases with soil depth, resulting in a higher biological activity and carbon fixation in the topcrust sections. The subcrust showed relative higher amounts of C_carb contributing to the soil surface stability. Depending on the relief position the total amount of accumulated carbon was 4 times

  20. Plastic Accumulation in the Mediterranean Sea

    PubMed Central

    Cózar, Andrés; Sanz-Martín, Marina; Martí, Elisa; González-Gordillo, J. Ignacio; Ubeda, Bárbara; Gálvez, José Á.; Irigoien, Xabier; Duarte, Carlos M.

    2015-01-01

    Concentrations of floating plastic were measured throughout the Mediterranean Sea to assess whether this basin can be regarded as a great accumulation region of plastic debris. We found that the average density of plastic (1 item per 4 m2), as well as its frequency of occurrence (100% of the sites sampled), are comparable to the accumulation zones described for the five subtropical ocean gyres. Plastic debris in the Mediterranean surface waters was dominated by millimeter-sized fragments, but showed a higher proportion of large plastic objects than that present in oceanic gyres, reflecting the closer connection with pollution sources. The accumulation of floating plastic in the Mediterranean Sea (between 1,000 and 3,000 tons) is likely related to the high human pressure together with the hydrodynamics of this semi-enclosed basin, with outflow mainly occurring through a deep water layer. Given the biological richness and concentration of economic activities in the Mediterranean Sea, the affects of plastic pollution on marine and human life are expected to be particularly frequent in this plastic accumulation region. PMID:25831129

  1. Plastic accumulation in the Mediterranean sea.

    PubMed

    Cózar, Andrés; Sanz-Martín, Marina; Martí, Elisa; González-Gordillo, J Ignacio; Ubeda, Bárbara; Gálvez, José Á; Irigoien, Xabier; Duarte, Carlos M

    2015-01-01

    Concentrations of floating plastic were measured throughout the Mediterranean Sea to assess whether this basin can be regarded as a great accumulation region of plastic debris. We found that the average density of plastic (1 item per 4 m2), as well as its frequency of occurrence (100% of the sites sampled), are comparable to the accumulation zones described for the five subtropical ocean gyres. Plastic debris in the Mediterranean surface waters was dominated by millimeter-sized fragments, but showed a higher proportion of large plastic objects than that present in oceanic gyres, reflecting the closer connection with pollution sources. The accumulation of floating plastic in the Mediterranean Sea (between 1,000 and 3,000 tons) is likely related to the high human pressure together with the hydrodynamics of this semi-enclosed basin, with outflow mainly occurring through a deep water layer. Given the biological richness and concentration of economic activities in the Mediterranean Sea, the affects of plastic pollution on marine and human life are expected to be particularly frequent in this plastic accumulation region. PMID:25831129

  2. Noise Reduction by Signal Accumulation

    ERIC Educational Resources Information Center

    Kraftmakher, Yaakov

    2006-01-01

    The aim of this paper is to show how the noise reduction by signal accumulation can be accomplished with a data acquisition system. This topic can be used for student projects. In many cases, the noise reduction is an unavoidable part of experimentation. Several techniques are known for this purpose, and among them the signal accumulation is the…

  3. 29 CFR 4219.14 - Amount of liability for 20-year-limitation amounts.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Amount of liability for 20-year-limitation amounts. 4219.14 Section 4219.14 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION WITHDRAWAL LIABILITY FOR MULTIEMPLOYER PLANS NOTICE, COLLECTION, AND REDETERMINATION OF WITHDRAWAL LIABILITY Redetermination of Withdrawal...

  4. 24 CFR 576.45 - Reallocation of grant amounts; returned or unused amounts.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 3 2011-04-01 2010-04-01 true Reallocation of grant amounts; returned or unused amounts. 576.45 Section 576.45 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING...

  5. 77 FR 59618 - Medicare Program; Medicare Appeals; Adjustment to the Amount in Controversy Threshold Amounts for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-28

    ... for requests for ALJ hearings and judicial review filed on or after January 1, 2013. The calendar year 2013 AIC threshold amounts are $140 for ALJ hearings and $1,400 for judicial review. Effective Date...) threshold amounts for Administrative Law Judge (ALJ) hearing requests and judicial review at $100 and...

  6. Calcium Oxalate Accumulation in Malpighian Tubules of Silkworm (Bombyx mori)

    NASA Astrophysics Data System (ADS)

    Wyman, Aaron J.; Webb, Mary Alice

    2007-04-01

    Silkworm provides an ideal model system for study of calcium oxalate crystallization in kidney-like organs, called Malpighian tubules. During their growth and development, silkworm larvae accumulate massive amounts of calcium oxalate crystals in their Malpighian tubules with no apparent harm to the organism. This manuscript reports studies of crystal structure in the tubules along with analyses identifying molecular constituents of tubule exudate.

  7. Adipocyte and leptin accumulation in tumor-induced thymic involution.

    PubMed

    Lamas, Alejandro; Lopez, Elena; Carrio, Roberto; Lopez, Diana M

    2016-01-01

    Cell-mediated immunity is an important defense mechanism against pathogens and developing tumor cells. The thymus is the main lymphoid organ involved in the formation of the cell-mediated immune response by the maturation and differentiation of lymphocytes that travel from the bone marrow, through the lymphatic ducts, to become T lymphocytes. Thymic involution has been associated with aging; however, other factors such as obesity, viral infection and tumor development have been shown to increase the rate of shrinkage of this organ. The heavy infiltration of adipocyte fat cells has been reported in the involuted thymuses of aged mice. In the present study, the possible accumulation of such cells in the thymus during tumorigenesis was examined by immunohistochemistry. A significant number of adipocytes around and infiltrating the thymuses of tumor-bearing mice was observed. Leptin is a pro-inflammatory adipocytokine that enhances thymopoiesis and modulates T cell immune responses. The levels of leptin and adiponectin, another adipocytokine that has anti-inflammatory properties, were examined by western blot analysis. While no changes were observed in the amounts of adiponectin present in the thymuses of the normal and tumor-bearing mice, significantly higher levels of leptin were detected in the thymocytes of the tumor-bearing mice. This correlated with an increase in the expression of certain cytokines, such as interleukin (IL)-2, interferon (IFN)-γ and granulocyte-macrophage colony-stimulating factor (GM-CSF). The co-culture of thymocytes isolated from normal mice with ex vivo isolated adipocytes from tumor-bearing mice yielded similar results. Our findings suggest that the infiltration and accumulation of adipocytes in the thymuses of tumor-bearing mice play an important role in their altered morphology and functions. PMID:26530443

  8. Neutron autoradiography of trace amounts of gadolinium

    SciTech Connect

    Shih, J.L.A.; Brugger, R.M. )

    1992-05-01

    This paper reports that application of gadolinium in neutron capture therapy is under evaluation. Crucial to development of this therapy is an imaging technique that would show the distribution and concentration of parts-per-million amounts of {sup 157}Gd in sliced samples. A technique that utilizes the principles of autoradiography and neutron radiography has been tried. The images taken with this method display the gadolinium distribution and its relative concentration in samples. Concentrations of {sup 157}Gd ranging from {approximately}20 to 500 ppm can be determined. The intrinsic spatial resolution of the system used in the Missouri University Research Reactor is 70 {mu}m.

  9. Inhibitory effect of mineral ion accumulation on high density growth of the hyperthermophilic archaeon Sulfolobus solfataricus.

    PubMed

    Park, C B; Lee, S B

    1999-01-01

    A fed-batch operation for high density cultivation of Sulfolobus solfataricus (DSM 1617) in a bench-top fermentor using a feed medium composed of glucose and yeast extract was investigated. The highest maximal cell density obtained in controlled fed-batch cultures was 21.7 g/l. Although higher yeast extract concentrations in the medium favored greater cell biomass yield, cell growth ceased with low cell densities. It was observed that large amounts of inorganic ions, such as sulfate, ammonium, potassium and phosphate ions, were accumulated in the culture broth at higher yeast extract concentrations. This was due to either the addition of the titrant or feeding of yeast extract during cultivation. Fed-batch cultures with additional mineral salts in the feed medium showed much lower cell biomass, indicating that accumulation of inorganic ions has a significant inhibitory effect on the growth of S. solfataricus. Inhibition of cell growth by the presence of mineral ions was further confirmed by the batch culture experiments. Some plausible mechanisms which can account for the growth inhibition at higher mineral ion concentrations have been suggested. PMID:16232474

  10. Accumulative Tritium Transfer from Water into Biosystems

    SciTech Connect

    Baumgaertner, Franz

    2005-07-15

    The energy balance of hydrogen isotopes in H bonds of water and biomolecules results in accumulative tritium transfer from water into biomolecules. Tests of DNA dissolved in tritiated water and of maize or barley hydroponically grown in tritiated water confirm the increase. The primary hydration shell of DNA shows an accumulation factor of {approx}1.4, and the exchangeable hydrogens inside DNA show {approx}2. Logistic growth analyses of maize and barley reveal the intrinsic growth rates of tritium 1.3 and 1.2 times larger than that of hydrogen. The higher rate of tritium than hydrogen incorporation in solid biomatter is caused by the hydration shells, which constitute an intrinsic component of biomolecules.

  11. Gypsum accumulation on carbonate stone

    USGS Publications Warehouse

    McGee, E.S.; Mossotti, V.G.

    1992-01-01

    The accumulation of gypsum on carbonate stone has been investigated through exposure of fresh samples of limestone and marble at monitored sites, through examination of alteration crusts from old buildings and through laboratory experiments. Several factors contribute to gypsum accumulation on carbonate stone. Marble or limestone that is sheltered from direct washing by rain in an urban environment with elevated pollution levels is likely to accumulate a gypsum crust. Crust development may be enhanced if the stone is porous or has an irregular surface area. Gypsum crusts are a surficial alteration feature; gypsum crystals form at the pore opening-air interface, where evaporation is greatest.

  12. Accumulation of poly (3-hydroxybutyric acid) by some soil Streptomyces.

    PubMed

    Manna, A; Banerjee, R; Paul, A K

    1999-09-01

    In a limited-scale survey, 55 soil streptomycetes were screened for the accumulation of poly (3-hydroxybutyrate) [PHB]. Only 18% of the isolates accumulated PHB ranging between 1.9-7.8% of the dry biomass. The promising isolate DBCC-719, identified as Streptomyces griseorubiginosus, accumulated PHB amounting to 9.5% of the mycelial dry mass in the early stationary phase when grown in chemically defined medium with 2% (wt/vol) glucose as the sole source of carbon. Nitrogen-limiting conditions were inhibitory to growth and PHB accumulation. The isolated polymer was highly soluble in chloroform, gave a sharp peak at 235 nm on digestion with concentrated H(2)SO(4), and had a characteristic infrared spectrum. PMID:10441729

  13. Accumulation of heavy metals in a long-term poultry waste-amended soil

    SciTech Connect

    Han, F.X.; Kingery, W.L.; Selim, H.M.; Gerard, P.D.

    2000-03-01

    Various metals are added to poultry diets to facilitate weight increase and disease prevention. The large amounts of poultry waste produced annually are dispersed intensively over relatively small areas of land, resulting in accumulations that pose potential environmental risks to the surface and groundwater. The focus of this study was to assess the distribution of heavy metals among various solid-phase fractions in soil profiles from a 25-year poultry waste-amended soil. Copper and Zn accumulated close to the soil surface where the total amounts of Cu and Zn in waste-amended soils were significantly higher than in nonamended soils. The total metal concentrations in amended soils were not critically high. Copper in the amended soil was present mostly in the organic matter (OM) fraction (46.9%), whereas Zn was found in the easily reducible oxide (ERO) fraction (47.3%). This suggests that the Cu and Zn in this long-term amended soil are potentially bioavailable and mobile. The authors observed the mobility of Zn through much of the soil profile of the long-term waste-amended soil. Zinc in this soil profile was found primarily in forms of the residual (RES) and crystalline iron oxide bound (CryFe) fractions, followed by the organic matter-bound and exchangeable (EXC) fractions.

  14. Toxic Takifugu pardalis eggs found in Takifugu niphobles gut: Implications for TTX accumulation in the pufferfish.

    PubMed

    Itoi, Shiro; Kozaki, Ao; Komori, Keitaro; Tsunashima, Tadasuke; Noguchi, Shunsuke; Kawane, Mitsuo; Sugita, Haruo

    2015-12-15

    Pufferfish (Takifugu spp.) possess a potent neurotoxin, tetrodotoxin (TTX). TTX has been detected in various organisms including food animals of pufferfish, and TTX-producing bacteria have been isolated from these animals. TTX in marine pufferfish accumulates in the pufferfish via the food web starting with marine bacteria. However, such accumulation is unlikely to account for the amount of TTX in the pufferfish body because of the minute amounts of TTX produced by marine bacteria. Therefore, the toxification process in pufferfish still remains unclear. In this article we report the presence of numerous Takifugu pardalis eggs in the intestinal contents of another pufferfish, Takifugu niphobles. The identity of T. pardalis being determined by direct sequencing for mitochondrial DNA. LC-MS/MS analysis revealed that the peak detected in the egg samples corresponded to TTX. Toxification experiments in recirculating aquaria demonstrated that cultured Takifugu rubripes quickly became toxic upon being fed toxic (TTX-containing) T. rubripes eggs. These results suggest that T. niphobles ingested the toxic eggs of another pufferfish T. pardalis to toxify themselves more efficiently via a TTX loop consisting of TTX-bearing organisms at a higher trophic level in the food web. PMID:26485535

  15. Total carbon accumulation in a tropical forest landscape

    PubMed Central

    2012-01-01

    Background Regrowing tropical forests worldwide sequester important amounts of carbon and restore part of the C emissions emitted by deforestation. However, there are large uncertainties concerning the rates of carbon accumulation after the abandonment of agricultural and pasture land. We report here accumulation of total carbon stocks (TCS) in a chronosequence of secondary forests at a mid-elevation landscape (900-1200 m asl) in the Andean mountains of Colombia. Results We found positive accumulation rates for all ecosystem pools except soil carbon, which showed no significant trend of recovery after 36 years of secondary succession. We used these data to develop a simple model to predict accumulation of TCS over time. This model performed remarkably well predicting TCS at other chronosequences in the Americas (Root Mean Square Error < 40 Mg C ha-1), which provided an opportunity to explore different assumptions in the calculation of large-scale carbon budgets. Simulations of TCS with our empirical model were used to test three assumptions often made in carbon budgets: 1) the use of carbon accumulation in tree aboveground biomass as a surrogate for accumulation of TCS, 2) the implicit consideration of carbon legacies from previous land-use, and 3) the omission of landscape age in calculating accumulation rates of TCS. Conclusions Our simulations showed that in many situations carbon can be released from regrowing secondary forests depending on the amount of carbon legacies and the average age of the landscape. In most cases, the rates used to predict carbon accumulation in the Americas were above the rates predicted in our simulations. These biome level rates seemed to be realistic only in landscapes not affected by carbon legacies from previous land-use and mean ages of around 10 years. PMID:23249727

  16. ACCUMULATION OF RADIOCESIUM BY MUSHROOMS IN THE ENVIRONMENT: A LITERATURE REVIEW AND IMAGE GALLERY

    SciTech Connect

    Duff, M; Mary Ramsey, M

    2006-11-05

    During the last 50 years, a large amount of information on radionuclide accumulators or 'sentinel-type' organisms in the environment has been published. Much of this work focused on the risks of food-chain transfer of radionuclides to higher organisms such as reindeer and man. However, until the 1980's and 1990's, there has been little published data on the radiocesium ({sup 134}Cs and {sup 137}Cs) accumulation by mushrooms. This presentation will consist of a review of the published data for {sup 134,137}Cs accumulation by mushrooms in nature. The review will consider the time of sampling, sample location characteristics, the radiocesium source term and other aspects that promote {sup 134,137}Cs uptake by mushrooms. This review will focus on published data for mushrooms that demonstrate a large propensity for use in the environmental biomonitoring of radiocesium contamination. It will also provide photographs and descriptions of habitats for many of these mushrooms to facilitate their collection for biomonitoring.

  17. Levels of Lycopene β-Cyclase 1 Modulate Carotenoid Gene Expression and Accumulation in Daucus carota

    PubMed Central

    Moreno, Juan Camilo; Pizarro, Lorena; Fuentes, Paulina; Handford, Michael; Cifuentes, Victor; Stange, Claudia

    2013-01-01

    Plant carotenoids are synthesized and accumulated in plastids through a highly regulated pathway. Lycopene β-cyclase (LCYB) is a key enzyme involved directly in the synthesis of α-carotene and β-carotene through the cyclization of lycopene. Carotenoids are produced in both carrot (Daucus carota) leaves and reserve roots, and high amounts of α-carotene and β-carotene accumulate in the latter. In some plant models, the presence of different isoforms of carotenogenic genes is associated with an organ-specific function. D. carota harbors two Lcyb genes, of which DcLcyb1 is expressed in leaves and storage roots during carrot development, correlating with an increase in carotenoid levels. In this work, we show that DcLCYB1 is localized in the plastid and that it is a functional enzyme, as demonstrated by heterologous complementation in Escherichia coli and over expression and post transcriptional gene silencing in carrot. Transgenic plants with higher or reduced levels of DcLcyb1 had incremented or reduced levels of chlorophyll, total carotenoids and β-carotene in leaves and in the storage roots, respectively. In addition, changes in the expression of DcLcyb1 are accompanied by a modulation in the expression of key endogenous carotenogenic genes. Our results indicate that DcLcyb1 does not possess an organ specific function and modulate carotenoid gene expression and accumulation in carrot leaves and storage roots. PMID:23555569

  18. Flavonoid accumulation in Arabidopsis repressed in lignin synthesis affects auxin transport and plant growth.

    PubMed

    Besseau, Sébastien; Hoffmann, Laurent; Geoffroy, Pierrette; Lapierre, Catherine; Pollet, Brigitte; Legrand, Michel

    2007-01-01

    In Arabidopsis thaliana, silencing of hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT), a lignin biosynthetic gene, results in a strong reduction of plant growth. We show that, in HCT-silenced plants, lignin synthesis repression leads to the redirection of the metabolic flux into flavonoids through chalcone synthase activity. Several flavonol glycosides and acylated anthocyanin were shown to accumulate in higher amounts in silenced plants. By contrast, sinapoylmalate levels were barely affected, suggesting that the synthesis of that phenylpropanoid compound might be HCT-independent. The growth phenotype of HCT-silenced plants was shown to be controlled by light and to depend on chalcone synthase expression. Histochemical analysis of silenced stem tissues demonstrated altered tracheary elements. The level of plant growth reduction of HCT-deficient plants was correlated with the inhibition of auxin transport. Suppression of flavonoid accumulation by chalcone synthase repression in HCT-deficient plants restored normal auxin transport and wild-type plant growth. By contrast, the lignin structure of the plants simultaneously repressed for HCT and chalcone synthase remained as severely altered as in HCT-silenced plants, with a large predominance of nonmethoxylated H units. These data demonstrate that the reduced size phenotype of HCT-silenced plants is not due to the alteration of lignin synthesis but to flavonoid accumulation. PMID:17237352

  19. Accumulation of radiocesium by mushrooms in the environment: a literature review.

    PubMed

    Duff, Martine C; Ramsey, Mary Lou

    2008-06-01

    During the last 50 years, a large amount of information on radionuclide accumulators or "sentinel-type" organisms in the environment has been published. Much of this work focused on the risks of food-chain transfer of radionuclides to higher organisms such as reindeer and man. Until the 1980s and 1990s, there were few published data on the radiocesium ((134)Cs and (137)Cs) accumulation by mushrooms. The present review of published data for (134,137)Cs accumulation by mushrooms in nature discusses the aspects that promote (134,137)Cs uptake by mushrooms and focuses on mushrooms that demonstrate a propensity for use in the environmental biomonitoring of radiocesium contamination. Transfer factors (TF, as dry weight concentration in fruiting body divided by concentration in substrate) ranged up to 24 (unitless), and aggregate transfer factors (T(ag), as Bq(137)Cs/kg dw in fruiting body divided by the aerial deposition as Bq/m(2)) ranged up to 8m(2)/kg dw. PMID:18191312

  20. 48 CFR 970.5215-1 - Total available fee: Base fee amount and performance fee amount.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... the conclusion of each specified evaluation period, evaluate the Contractor's performance of all... available fee amount earned. At the Contracting Officer's discretion, evaluation of incentivized performance... Contractor performance shall be in accordance with the Performance Evaluation and Measurement...

  1. Manganese As a Metal Accumulator

    EPA Science Inventory

    Manganese deposits in water distribution systems accumulate metals, radionuclides and oxyanions by a combination of surface complexation, adsorption and solid substitution, as well as a combination of oxidation followed by manganese reduction and sorption of the oxidized constitu...

  2. Evidence accumulation for spatial reasoning

    NASA Technical Reports Server (NTRS)

    Matsuyama, T.; Hwang, V. S. S.; Davis, L. S.

    1984-01-01

    The evidence accumulation proces of an image understanding system is described enabling the system to perform top-down(goal-oriented) picture processing as well as bottom-up verification of consistent spatial relations among objects.

  3. Molybdenum accumulation and storage in Klebsiella pneumoniae and Azotobacter vinelandii.

    PubMed Central

    Pienkos, P T; Brill, W J

    1981-01-01

    In Klebsiella pneumoniae, Mo accumulation appeared to be coregulated with nitrogenase synthesis. O2 and NH+4, which repressed nitrogenase synthesis, also prevented Mo accumulation. In Azotobacter vinelandii, Mo accumulation did not appear to be regulated Mo was accumulated to levels much higher than those seen in K. pneumoniae even when nitrogenase synthesis was repressed. Accumulated Mo was bound mainly to a Mo storage protein, and it could act as a supply for the Mo needed in component I synthesis when extracellular Mo had been exhausted. When A. vinelandii was grown in the presence of WO2-(4) rather than MoO2-(4), it synthesized a W-containing analog of the Mo storage protein. The Mo storage protein was purified from both NH+4 and N2-grown cells of A. vinelandii and found to be a tetramer of two pairs of different subunits binding a minimum of 15 atoms of Mo per tetramer. Images PMID:7007348

  4. Molybdenum accumulation and storage in Klebsiella pneumoniae and Azotobacter vinelandii.

    PubMed

    Pienkos, P T; Brill, W J

    1981-02-01

    In Klebsiella pneumoniae, Mo accumulation appeared to be coregulated with nitrogenase synthesis. O2 and NH+4, which repressed nitrogenase synthesis, also prevented Mo accumulation. In Azotobacter vinelandii, Mo accumulation did not appear to be regulated Mo was accumulated to levels much higher than those seen in K. pneumoniae even when nitrogenase synthesis was repressed. Accumulated Mo was bound mainly to a Mo storage protein, and it could act as a supply for the Mo needed in component I synthesis when extracellular Mo had been exhausted. When A. vinelandii was grown in the presence of WO2-(4) rather than MoO2-(4), it synthesized a W-containing analog of the Mo storage protein. The Mo storage protein was purified from both NH+4 and N2-grown cells of A. vinelandii and found to be a tetramer of two pairs of different subunits binding a minimum of 15 atoms of Mo per tetramer. PMID:7007348

  5. Accumulation of dieldrin in an alga (Scenedesmus obliquus), Daphnia magna, and the guppy (Poecilia reticulata)

    USGS Publications Warehouse

    Reinert, Robert E.

    1972-01-01

    Scenedesmus obliquus, Daphnia magna, and Poecilia reticulata accumulated dieldrin directly from water; average concentration factors (concentration in organism, dry weight, divided by concentration in water) were 1282 for the alga, 13,954 for D. magna, and 49,307 (estimated) for the guppy. The amount accumulated by each species at equilibrium (after about 1.5, 3-4, and 18 days, respectively) was directly proportional to the concentration of dieldrin in the water. Daphnia magna and guppies accumulated more dieldrin from water than from food that had been exposed to similar concentrations in water. When guppies were fed equal daily rations of D. magna containing different concentrations of insecticide, the amounts of dieldrin accumulated by the fish were directly proportional to the concentration in D. magna; when two lots of guppies were fed different quantities of D. magna (10 and 20 organisms per day) containing identical concentrations of dieldrin, however, the amounts accumulated did not differ substantially.

  6. Modeling degradation ratios and nutrient availability of anthocyanidin-accumulating Lc-alfalfa populations in dairy cows.

    PubMed

    Jonker, A; Gruber, M Y; Wang, Y; Coulman, B; Azarfar, A; McKinnon, J J; Christensen, D A; Yu, P

    2011-03-01

    Dairy cattle eating fresh or ensiled alfalfa as the main portion of their diet often have low protein efficiency because of the rapid initial rate of ruminal protein degradation of fresh and ensiled alfalfa. Ruminal protein degradation of alfalfa might be reduced by introducing a gene that stimulates the accumulation of mono- or polymeric anthocyanidins in alfalfa. The objectives of this study were to fractionate protein and carbohydrates by in situ and chemical approaches, to evaluate in situ ruminal degradation characteristics and synchronization ratios, to determine protein availability to dairy cattle using the 2007 digestible intestinal protein/rumen-degraded protein balance (DVE/OEB) protein system, and to determine net energy for lactation using the Dutch net energy for lactation (VEM) system for 3 newly developed transgenic winter hardy anthocyanidin-accumulating T(1)Lc-alfalfa populations. These T(1)Lc-alfalfa populations, called (T1)BeavLc1, (T1)RambLc3, and (T1)RangLc4, had an average anthocyanidin accumulation of 163.4 μg/g of DM, whereas AC Grazeland (selected for a low initial rate of degradation) did not accumulate anthocyanidin. The basic chemical composition of the original samples, soluble and potentially degradable fractions, and degradation characteristics of crude protein and carbohydrates were similar in T(1)Lc-alfalfa and AC Grazeland. The undegradable in situ crude protein and neutral detergent fiber fraction had 1.3% lower CP and 4.8% lower CHO, respectively, in T(1)Lc-alfalfa compared with the amounts in AC Grazeland. The T(1)Lc-alfalfa had a 0.34 MJ/kg of DM higher calculated net energy for lactation and 1.9% of CP higher buffer soluble protein compared with that in AC Grazeland. By the protein evaluation model, it was predicted that T(1)Lc-alfalfa tended to have 11.9, 6.9, and 8.4 g/kg of DM higher rumen degradable protein, OEB, and intestinal available protein, respectively, compared with the amounts in AC Grazeland. The hourly OEB

  7. [Absorption and accumulation of heavy metals by plants around a smelter].

    PubMed

    Cui, Shunag; Zhou, Qixing; Chao, Lei

    2006-03-01

    The study on the absorption and accumulation of heavy metals lead, zinc, copper and cadmium by 8 plant species around a smelter showed that the metals accumulation by plants differed with plant species, their parts, and kinds of metals. Abutilon theophrasti had a higher capability of absorbing and accumulating Pb, Conyza canadensis, Ambrosia trifida, Polygonumn lapathifolium, A. theophrasti, Solanum nigrum, Chenopodium acuminatum and Helianthus tuberosus had a higher capability of absorbing and accumulating Zn, C. acuminatunz and A. theophrasti had a higher capability of absorbing and accumulating Cu, and S. nigrum, C. acuminatum, A. theophrasti, P. lapathifolium and C. canadensis had a higher capability of absorbing and accumulating Cd. These plants had TF values higher than 1, and were suitable for phytoextraction to remedy polluted soil. As for the plants with TF values lower than 1, they were suitable as the phytostabilizers of heavy metals-contaminated lands. PMID:16724753

  8. Icaritin Inhibits Collagen Degradation-Related Factors and Facilitates Collagen Accumulation in Atherosclerotic Lesions: A Potential Action for Plaque Stabilization

    PubMed Central

    Zhang, Zong-Kang; Li, Jie; Yan, De-Xin; Leung, Wing-Nang; Zhang, Bao-Ting

    2016-01-01

    Most acute coronary syndromes result from rupture of vulnerable atherosclerotic plaques. The collagen content of plaques may critically affect plaque stability. This study tested whether Icaritin (ICT), an intestinal metabolite of Epimedium-derived flavonoids, could alter the collagen synthesis/degradation balance in atherosclerotic lesions. Rabbits were fed with an atherogenic diet for four months. Oral administration of ICT (10 mg·kg−1·day−1) was started after two months of an atherogenic diet and lasted for two months. The collagen degradation-related parameters, including macrophages accumulation, content and activity of interstitial collagenase-1 (MMP-1), and the collagen synthesis-related parameters, including amount and distribution of smooth muscle cells (SMC) and collagen mRNA/protein levels, were evaluated in the aorta. ICT reduced plasma lipid levels, inhibited macrophage accumulation, lowered MMP-1 mRNA and protein expression, and suppressed proteolytic activity of pro-MMP-1 and MMP-1 in the aorta. ICT changed the distribution of the SMCs towards the fibrous cap of lesions without increasing the amount of SMCs. Higher collagen protein content in lesions and aorta homogenates was observed with ICT treatment compared with the atherogenic diet only, without altered collagen mRNA level. These results suggest that ICT could inhibit the collagen degradation-related factors and facilitate collagen accumulation in atherosclerotic lesions, indicating a new potential of ICT in atherosclerotic plaques. PMID:26828485

  9. Accumulation and sub-cellular partitioning of metals and As in the clam Venerupis corrugata: Different strategies towards different elements.

    PubMed

    Velez, Cátia; Figueira, Etelvina; Soares, Amadeu M V M; Freitas, Rosa

    2016-08-01

    The main goal of the present study was to assess accumulation, tolerance and sub-cellular partitioning of As, Hg, Cd and Pb in Venerupis corrugata. Results showed an increase of elements accumulation in V. corrugata with the increase of exposure. However, organisms presented higher capacity to accumulate Hg, Cd and Pb (BCF ≥ 12.8) than As (BCF ≤ 2.1) and higher accumulation rate for Cd and Pb than for Hg and As. With the increase of Hg exposure concentrations clams tended to increase the amount of metal bound to metal-sensitive fractions, which may explain the mortality recorded at the highest exposure concentration. Cd sub-cellular partitioning showed that with the increase of exposure concentrations V. corrugata increased the amount of metal in the cellular debris fraction, probably bound to the cellular membranes which explain the mortality recorded at the highest concentration. Results on As partitioning demonstrated that most of the metalloid was associated with fractions in the biologically detoxified metal compartment (BDM). Since high mortality was observed in clams exposed to As our results may indicate that this strategy was not enough to prevent clams from toxic effects and mortality occurred. When exposed to Pb most of the metal was in the BDM compartment, but in this case the metal was mostly in the metal-rich granules fraction which seemed to be efficient in preventing clams from toxicity, and no mortality was recorded. Our study further revealed that As and Hg were the most available elements to be biomagnified through the food chain. PMID:27174825

  10. Characteristics of nonylphenol and bisphenol A accumulation by fish and implications for ecological and human health.

    PubMed

    Lee, Ching-Chang; Jiang, Ling-Ying; Kuo, Yi-Ling; Chen, Chung-Yu; Hsieh, Chia-Yi; Hung, Chung-Feng; Tien, Chien-Jung

    2015-01-01

    Fish populations constitute an important part of aquatic ecosystems. Thus, their accumulation of nonylphenol (NP) and bisphenol A (BPA) may pose risks to ecosystems and human health. This study analyzed the concentrations of NP and BPA in four types of fishes (i.e., wild/farmed freshwater fishes and wild/farmed marine fishes). Wild freshwater fishes contained higher concentrations of NP and BPA than the other three types of fishes. The concentrations of NP in the wild freshwater fishes ranged from 1.01 to 277 μg/kg ww, with bioconcentration factors (BCFs) and biota-sediment accumulation factors (BSAFs) ranging from 74.0 to 2.60 × 10(4)L/kg and from 0.003 to 18.3, respectively. The wild freshwater fishes contained relatively low amounts of BPA, varying from ND to 25.2 μg/kg ww, with the BCFs and BSAFs ranging from 1.00 to 274L/kg and from 0.003 to 3.40, respectively. Five fish species particularly showed high BCFs and BSAFs, indicating that they could be an important source of NP for higher trophic levels, most likely resulting in ecological risks. The demersal fishes showed a greater ability to accumulate NP than the pelagic ones. The fact that the 95th percentile values of the risk quotient (RQ) for NP and BPA were higher than the acceptable threshold indicated that these two compounds would have adverse effects on aquatic organisms in Taiwanese rivers. The consumption of wild marine fishes had the highest 95th percentile values of hazard quotient (HQ) for NP and BPA among the four types of fishes, particularly for the population aged 0-3 years. However, the 95th percentile values of HQ for NP and BPA were all less than 1, suggesting that exposure to NP and BPA through fish consumption posed no remarkable risk to human health in Taiwan. PMID:25268571

  11. Change in Uptake, Transport and Accumulation of Ions in Nerium oleander (Rosebay) as Affected by Different Nitrogen Sources and Salinity

    PubMed Central

    Abdolzadeh, Ahmad; Shima, Kazuto; Lambers, Hans; Chiba, Kyozo

    2008-01-01

    Background and Aims The source of nitrogen plays an important role in salt tolerance of plants. In this study, the effects of NaCl on net uptake, accumulation and transport of ions were investigated in Nerium oleander with ammonium or nitrate as the nitrogen source in order to analyse differences in uptake and cycling of ions within plants. Methods Plants were grown in a greenhouse in hydroponics under different salt treatments (control vs. 100 mm NaCl) with ammonium or nitrate as the nitrogen source, and changes in ion concentration in plants, xylem sap exuded from roots and stems, and phloem sap were determined. Key Results Plant weight, leaf area and photosynthetic rate showed a higher salt tolerance of nitrate-fed plants compared with that of ammonium-fed plants. The total amount of Na+ transported in the xylem in roots, accumulated in the shoot and retranslocated in the phloem of ammonium-fed plants under salt treatment was 1·8, 1·9 and 2·7 times more, respectively, than that of nitrate-treated plants. However, the amount of Na+ accumulated in roots in nitrate-fed plants was about 1·5 times higher than that in ammonium-fed plants. Similarly, Cl− transport via the xylem to the shoot and its retranslocation via the phloem (Cl− cycling) were far greater with ammonium treatment than with nitrate treatment under conditions of salinity. The uptake and accumulation of K+ in shoots decreased more due to salinity in ammonium-fed plants compared with nitrate-fed plants. In contrast, K+ cycling in shoots increased due to salinity, with higher rates in the ammonium-treated plants. Conclusions The faster growth of nitrate-fed plants under conditions of salinity was associated with a lower transport and accumulation of Na+ and Cl− in the shoot, whereas in ammonium-fed plants accumulation and cycling of Na+ and Cl− in shoots probably caused harmful effects and reduced growth of plants. PMID:18772147

  12. Nuclear DNA Amounts in Macaronesian Angiosperms

    PubMed Central

    SUDA, JAN; KYNCL, TOMÁŠ; FREIOVÁ, RADKA

    2003-01-01

    Nuclear DNA contents for 104 Macaronesian angiosperms, with particular attention on Canary Islands endemics, were analysed using propidium iodide flow cytometry. Prime estimates for more than one‐sixth of the whole Canarian endemic flora (including representatives of 11 endemic genera) were obtained. The resulting 1C DNA values ranged from 0·19 to 7·21 pg for Descurainia bourgeauana and Argyranthemum frutescens, respectively (about 38‐fold difference). The majority of species, however, possessed (very) small genomes, with C‐values <1·6 pg. The tendency towards small nuclear DNA contents and genome sizes was confirmed by comparing average values for Macaronesian and non‐Macaronesian representatives of individual families, genera and major phylogenetic lineages. Our data support the hypothesis that the insular selection pressures in Macaronesia favour small C‐values and genome sizes. Both positive and negative correlations between infrageneric nuclear DNA amount variation and environmental conditions on Tenerife were also found in several genera. PMID:12824074

  13. Extrapulmonary sites of radiogallium accumulation in sarcoidosis

    SciTech Connect

    Sulavik, S.B.; Palestro, C.J.; Spencer, R.P.; Swyer, A.J.; Goldsmith, S.J.; Tierstein, A.S. )

    1990-12-01

    In an effort to detect extrapulmonary sites of radiogallium accumulation in cases of sarcoidosis, 145 separate Ga-67 citrate studies of 114 patients with biopsy-proven sarcoidosis were examined. The most characteristic extrapulmonary radiogallium uptake pattern was the panda sign in 47 patients (41%). The most common site of prominent extrapulmonary radiogallium uptake was the lacrimal glands in 101 patients (88%). Second most common was activity in one or more superficial lymph node regions such as the cervical, axillary, femoral, or inguinal in 19 patients (17%). Other extrapulmonary sites included breast uptake in 6 out of 80 women (8%), prominent splenic and nasal uptake in 9 (8%) patients, periportal accumulation in 7 (6%), and cutaneous/subcutaneous activity in 4 (4%). Because many of these individuals were receiving corticosteroids, the natural (untreated) prevalence of extrapulmonary findings may be even higher. Although the sensitivity and specificity of extrapulmonary radiogallium accumulation has still to be determined, many of the sites may be accessible to biopsy both for diagnostic purposes and to follow the effects of medications. It is therefore suggested that whole-body imaging be performed when radiogallium is administered to patients with suspected or known sarcoidosis.

  14. 17 CFR 210.12-28 - Real estate and accumulated depreciation. 1

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 17 Commodity and Securities Exchanges 3 2014-04-01 2014-04-01 false Real estate and accumulated... AND CONSERVATION ACT OF 1975 For Certain Real Estate Companies § 210.12-28 Real estate and accumulated..., furnish a reconciliation, in the following form, of the total amount at which real estate was carried...

  15. 17 CFR 210.12-28 - Real estate and accumulated depreciation. 1

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 17 Commodity and Securities Exchanges 2 2012-04-01 2012-04-01 false Real estate and accumulated... AND CONSERVATION ACT OF 1975 Form and Content of Schedules § 210.12-28 Real estate and accumulated..., furnish a reconciliation, in the following form, of the total amount at which real estate was carried...

  16. 17 CFR 210.12-28 - Real estate and accumulated depreciation. 1

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 17 Commodity and Securities Exchanges 2 2013-04-01 2013-04-01 false Real estate and accumulated... AND CONSERVATION ACT OF 1975 Form and Content of Schedules § 210.12-28 Real estate and accumulated..., furnish a reconciliation, in the following form, of the total amount at which real estate was carried...

  17. 18 CFR 367.2283 - Account 228.3, Accumulated provision for pensions and benefits.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Accumulated provision for pensions and benefits. 367.2283 Section 367.2283 Conservation of Power and Water....2283 Account 228.3, Accumulated provision for pensions and benefits. (a) This account must include provisions made by the service company and amounts contributed by employees for pensions, accident and...

  18. 18 CFR 367.2283 - Account 228.3, Accumulated provision for pensions and benefits.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Accumulated provision for pensions and benefits. 367.2283 Section 367.2283 Conservation of Power and Water....2283 Account 228.3, Accumulated provision for pensions and benefits. (a) This account must include provisions made by the service company and amounts contributed by employees for pensions, accident and...

  19. Accumulation of the Antibiotic Phenazine-1-Carboxylic Acid in the Rhizosphere of Dryland Cereals

    PubMed Central

    Mavrodi, Dmitri V.; Mavrodi, Olga V.; Parejko, James A.; Bonsall, Robert F.; Kwak, Youn-Sig; Paulitz, Timothy C.; Weller, David M.

    2012-01-01

    Natural antibiotics are thought to function in the defense, fitness, competitiveness, biocontrol activity, communication, and gene regulation of microorganisms. However, the scale and quantitative aspects of antibiotic production in natural settings are poorly understood. We addressed these fundamental questions by assessing the geographic distribution of indigenous phenazine-producing (Phz+) Pseudomonas spp. and the accumulation of the broad-spectrum antibiotic phenazine-1-carboxylic acid (PCA) in the rhizosphere of wheat grown in the low-precipitation zone (<350 mm) of the Columbia Plateau and in adjacent, higher-precipitation areas. Plants were collected from 61 commercial wheat fields located within an area of about 22,000 km2. Phz+ Pseudomonas spp. were detected in all sampled fields, with mean population sizes ranging from log 3.2 to log 7.1 g−1 (fresh weight) of roots. Linear regression analysis demonstrated a significant inverse relationship between annual precipitation and the proportion of plants colonized by Phz+ Pseudomonas spp. (r2 = 0.36, P = 0.0001). PCA was detected at up to nanomolar concentrations in the rhizosphere of plants from 26 of 29 fields that were selected for antibiotic quantitation. There was a direct relationship between the amount of PCA extracted from the rhizosphere and the population density of Phz+ pseudomonads (r2 = 0.46, P = 0.0006). This is the first demonstration of accumulation of significant quantities of a natural antibiotic across a terrestrial ecosystem. Our results strongly suggest that natural antibiotics can transiently accumulate in the plant rhizosphere in amounts sufficient not only for inter- and intraspecies signaling but also for the direct inhibition of sensitive organisms. PMID:22138981

  20. Naturally evolved enhanced Cd tolerance of Dianthus carthusianorum L. is not related to accumulation of thiol peptides and organic acids.

    PubMed

    Wójcik, Małgorzata; Dresler, Sławomir; Plak, Andrzej; Tukiendorf, Anna

    2015-05-01

    Two contrasting ecotypes of Dianthus carthusianorum L., metallicolous (M) and nonmetallicolous (NM), were cultivated in hydroponics at 0-50 μM Cd for 14 days to compare their Cd accumulation, sensitivity and tolerance mechanisms. While both ecotypes contained similar concentrations of Cd in the shoots and roots, the M ecotype was more Cd-tolerant (as measured by fresh weight production and root and leaf viability). Both ecotypes accumulated phytochelatins (PCs) in response to Cd with a higher amount thereof found in the NM ecotype. Concentrations of PCs remained unchanged with increasing Cd concentrations in the root tissues, but their content in the shoots increased. The addition of L-buthionine-sulfoximine (BSO) diminished glutathione (GSH) accumulation and arrested PC production, which increased the sensitivity to Cd of the NM, but not M ecotype. Organic acids (malate and citrate) as well as proline accumulation did not change significantly after Cd exposition and was at the same level in both ecotypes. The enhanced Cd tolerance of the M ecotype of D. carthusianorum cannot be explained in terms of restricted Cd uptake and differential production of PCs, organic acids or proline; some other mechanisms must be involved in its adaptation to the high Cd content in the environment. PMID:25510617

  1. Effect of temperature on accumulation of methylmercuric chloride and p,p'DDT by rainbow trout (Salmo gairdneri)

    USGS Publications Warehouse

    Reinert, Robert E.; Stone, Linda J.; Willford, Wayne A.

    1974-01-01

    Amounts of mercury and DDT residues accumulated from water by yearling rainbow trout (Salmo gairdneri) in the laboratory increased as water temperature increased. Fish exposed to methylmercuric chloride at concentrations of 234–263 parts per trillion for 12 wk at 5, 10, and 15 C accumulated 1.19, 1.71, and 1.96 ppm; fish exposed to p,p′DDT at concentrations of 133–176 parts per trillion accumulated 3.76, 5.93, and 6.82 ppm. Concentrations of mercury accumulated by the fish were significantly different (P < 0.01) at each of the three temperatures, and the concentrations of DDT were significantly different at 5 and 10 and 5 and 15 C. Throughout the period of exposure, the concentration factors (concentration of contaminant in the fish/concentration in water) at each of the three temperatures were far higher for p,p′DDT than for methylmercuric chloride.

  2. Elastic fiber assembly is disrupted by excessive accumulation of chondroitin sulfate in the human dermal fibrotic disease, keloid.

    PubMed

    Ikeda, Mika; Naitoh, Motoko; Kubota, Hiroshi; Ishiko, Toshihiro; Yoshikawa, Katsuhiro; Yamawaki, Satoko; Kurokawa, Masato; Utani, Atsushi; Nakamura, Tomoyuki; Nagata, Kazuhiro; Suzuki, Shigehiko

    2009-12-25

    Keloid is a fibrotic disease characterized by abnormal accumulation of extracellular matrix in the dermis. The keloid matrix contains excess collagen and glycosaminoglycans (GAGs), but lacks elastic fiber. However, the roles of these matrix components in the pathogenesis of keloid are largely unknown. Here, we show that elastin and DANCE (also known as fibulin-5), a protein required for elastic fiber formation, are not deposited in the extracellular matrix of keloids, due to excess accumulation of chondoitin sulfate (CS), although the expression of elastin and DANCE is not affected. Amount of CS accumulated in the keloid legion was 6.9-fold higher than in normal skin. Fibrillin-1, a scaffold protein for elastic fiber assembly, was abnormally distributed in the keloid matrix. Addition of purified CS to keloid fibroblast culture resulted in abnormal deposition of fibrillin-1, concomitant with significantly decreased accumulation of elastin and DANCE in the extracellular matrix. We propose that CS plays a crucial role in the development of keloid lesions through inhibition of elastic fiber assembly. PMID:19879246

  3. Accumulation and Distribution of Lead and Chromium in Laboratory-Scale Constructed Wetlands Inoculated with Metal-Tolerant Bacteria.

    PubMed

    Amabilis-Sosa, Leonel E; Siebe, Christina; Moeller-Chávez, Gabriela; Durán-Domínguez-de-Bazúa, María del Carmen

    2015-01-01

    The accumulation and distribution of lead and chromium was tested in a laboratory-scale constructed wetland (CW) inoculated with metal-tolerant bacteria. Two non-inoculated systems also were evaluated, one planted and the other unplanted. Mass balances indicated that 57% of chromium input was accumulated into inoculated CW after 151 days of operation. The distribution was similar in support media and vegetation, in which 78% was transferred to aerial part. Similarly Pb was accumulated 29% in the support media and 39% in vegetation, which was distributed 52% in rhizome and 48% in aerial part. Significantly lower amounts of heavy metals were accumulated in non-inoculated systems than in the inoculated wetlands (p < 0.005). In addition, a markedly higher proportion of chromium in aerial vegetation and of lead in the suspended fraction of the effluent was exhibited, which raises a subsequent recovery of the metal by harvest and settling, respectively. Results indicate that CW inoculated with metal-tolerant bacteria might be a suitable option for treating wastewater with content of lead and chromium. PMID:26023800

  4. Accumulation of Poly (beta-Hydroxybutyrate) by Halobacteria.

    PubMed

    Fernandez-Castillo, R; Rodriguez-Valera, F; Gonzalez-Ramos, J; Ruiz-Berraquero, F

    1986-01-01

    Some species of extremely halophilic archaebacteria, Halobacteriaceae, have been shown to accumulate large amounts of poly (beta-hydroxybutyrate) under conditions of nitrogen limitation and abundant carbon source. The production of poly (beta-hydroxybutyrate), at least in large quantities, was restricted to two carbohydrate-utilizing species, Halobacterium mediterranei and H. volcanii. In addition to the nutrients in the media, the salt concentration also influenced poly (beta-hydroxybutyrate) accumulation, which was greater at lower salt concentrations. The possible application of these microorganisms for the production of biodegradable plastics is discussed. PMID:16346972

  5. Optimum Tolerance Design Using Component-Amount and Mixture-Amount Experiments

    SciTech Connect

    Piepel, Gregory F.; Ozler, Cenk; Sehirlioglu, Ali Kemal

    2013-08-01

    One type of tolerance design problem involves optimizing component and assembly tolerances to minimize the total cost (sum of manufacturing cost and quality loss). Previous literature recommended using traditional response surface (RS) designs and models to solve this type of tolerance design problem. In this article, component-amount (CA) and mixture-amount (MA) approaches are proposed as more appropriate for solving this type of tolerance design problem. The advantages of the CA and MA approaches over the RS approach are discussed. Reasons for choosing between the CA and MA approaches are also discussed. The CA and MA approaches (experimental design, response modeling, and optimization) are illustrated using real examples.

  6. Arsenic accumulation by edible aquatic macrophytes.

    PubMed

    Falinski, K A; Yost, R S; Sampaga, E; Peard, J

    2014-01-01

    Edible aquatic macrophytes grown in arsenic (As)-contaminated soil and sediment were investigated to determine the extent of As accumulation and potential risk to humans when consumed. Nasturtium officinale (watercress) and Diplazium esculentum (warabi) are two aquatic macrophytes grown and consumed in Hawaii. Neither has been assessed for potential to accumulate As when grown in As-contaminated soil. Some former sugarcane plantation soils in eastern Hawaii have been shown to have concentrations of total As over 500 mg kg(-1). It was hypothesized that both species will accumulate more As in contaminated soils than in non-contaminated soils. N. officinale and D. esculentum were collected in areas with and without As-contaminated soil and sediment. High soil As concentrations averaged 356 mg kg(-1), while low soil As concentrations were 0.75 mg kg(-1). Average N. officinale and D. esculentum total As concentrations were 0.572 mg kg(-1) and 0.075 mg kg(-1), respectively, corresponding to hazard indices of 0.12 and 0.03 for adults. Unlike previous studies where watercress was grown in As-contaminated water, N. officinale did not show properties of a hyperaccumulator, yet plant concentrations in high As areas were more than double those in low As areas. There was a slight correlation between high total As in sediment and soil and total As concentrations in watercress leaves and stems, resulting in a plant uptake factor of 0.010, an order of magnitude higher than previous studies. D. esculentum did not show signs of accumulating As in the edible fiddleheads. Hawaii is unique in having volcanic ash soils with extremely high sorption characteristics of As and P that limit release into groundwater. This study presents a case where soils and sediments were significantly enriched in total As concentration, but the water As concentration was below detection limits. PMID:24210365

  7. The relation between lateralisation, early start of training, and amount of practice in musicians: a contribution to the problem of handedness classification.

    PubMed

    Kopiez, Reinhard; Galley, Niels; Lehmann, Andreas C

    2010-07-01

    This study investigates the influence of extensive bimanual training in professional musicians on the incidence of handedness in the most basic form of right-handedness (RH) and non-right-handedness (NRH), according to Annett's "right shift theory". The lateralisation coefficients (LCs) of a total sample of 128 bimanually performing music students were calculated for speed, regularity, and fatigue of tapping by using the speed tapping paradigm. Additionally, the accumulated amount of practice was recorded by means of retrospective interviews. The proportion of designated right-handers (dRH) and non-right-handers (dNRH) in hand performance was identified by binary logistic regression from LCs. A proportion of 30.8% designated NRH in the group of musicians was found, while in the control group of non-musicians (matched for age range) a proportion of 21.7% designated NRH was observed. Incidence of dNRH was higher in string players (35.6%) than in pianists (27.1%). As an effect of the extensive training of the left hand, tapping regularity increased and tapping fatigue decreased among those participants who evidenced an increased amount of accumulated practice time on the instrument. However, speed difference between hands (as indicated by LCs) remained uninfluenced by bimanual training. This finding is in contrast to those of Jancke, Schlaug, and Steinmetz (1997). Finally, our study provides a more reliable (statistical) classification as an external criterion for future genetic analyses of handedness. PMID:19462271

  8. Seed sprout production: Consumables and a foundation for higher plant growth in space

    NASA Technical Reports Server (NTRS)

    Day, Michelle; Thomas, Terri; Johnson, Steve; Luttges, Marvin

    1990-01-01

    Seed sprouts can be produced as a source of fresh vegetable materials and as higher plant seedlings in space. Sprout production was undertaken to evaluate the mass accumulations possible, the technologies needed, and the reliability of the overall process. Baseline experiments corroborated the utility of sprout production protocols for a variety of seed types. The automated delivery of saturated humidity effectively supplants labor intensive manual soaking techniques. Automated humidification also lend itself to modest centrifugal sprout growth environments. A small amount of ultraviolet radiation effectively suppressed bacterial and fungal contamination, and the sprouts were suitable for consumption.

  9. Accumulation and distribution characteristics of zinc and cadmium in the hyperaccumulator plant Sedum plumbizincicola.

    PubMed

    Cao, Dong; Zhang, Hongzheng; Wang, Yaodong; Zheng, Leina

    2014-08-01

    Accumulation and distribution of Zn and Cd in the hyperaccumulator plant Sedum plumbizincicola were investigated in a hydroponic experiment. Mean Cd and Zn concentrations in shoots (7,010 and 18,400 mg kg(-1)) were about sevenfold and fivefold higher than those in roots (840 and 3,000 mg kg(-1)) after exposure to 100 μM CdSO4 and 600 μM ZnSO4, respectively. Cd and Zn concentrations in young leaves (4,330 and 9,820 mg kg(-1)) were about sixfold and twofold higher than those in mature leaves (636 and 2,620 mg kg(-1)), respectively. MicroPIXE analysis showed that Zn was predominantly localized in epidermal cells in both young and mature leaves, but large amounts of Zn occurred in mesophyll cells in young leaves. Leaf tissue fractionation showed that soluble and cell wall fractions were different at the two stages of leaf growth. Young and mature leaves of S. plumbizincicola also showed different accumulation and distribution characteristics for Zn and Cd. PMID:24789526

  10. Manganese accumulation in yeast cells. Electron-spin-resonance characterization and superoxide dismutase activity.

    PubMed

    Galiazzo, F; Pedersen, J Z; Civitareale, P; Schiesser, A; Rotilio, G

    1989-01-01

    Manganese accumulation was studied by room-temperature electron spin resonance (ESR) spectroscopy in Saccharomyces cerevisiae grown in the presence of increasing amounts of MnSO4. Mn2+ retention was nearly linear in intact cells for fractions related to both low-molecular-mass and macromolecular complexes ('free' and 'bound' Mn2+, respectively). A deviation from linearity was observed in cell extracts between the control value and 0.1 mM Mn2+, indicating more efficient accumulation at low Mn2+ concentrations. The difference in slopes between the two straight lines describing Mn2+ retention at concentrations lower and higher than 0.1 mM, respectively, was quite large for the free Mn2+ fraction. Furthermore it was unaffected by subsequent dialyses of the extracts, showing stable retention in the form of low-molecular-mass complexes. In contrast, the slope of the line describing retention of 'bound' Mn2+ at concentrations higher than 0.1 mM became less steep after subsequent dialyses of the cell extracts. This result indicates that the macromolecule-bound Mn2+ was essentially associated with particulate structures. In contrast to Cu2+, Mn2+ had no effect on the major enzyme activities involved in oxygen metabolism except for a slight increase of cyanide-resistant Mn-superoxide dismutase activity, due to dialyzable Mn2+ complexes. PMID:2562042

  11. A review on soil carbon accumulation due to the management change of major Brazilian agricultural activities.

    PubMed

    La Scala jr, N; De Figueiredo, E B; Panosso, A R

    2012-08-01

    Agricultural areas deal with enormous CO2 intake fluxes offering an opportunity for greenhouse effect mitigation. In this work we studied the potential of soil carbon sequestration due to the management conversion in major agricultural activities in Brazil. Data from several studies indicate that in soybean/maize, and related rotation systems, a significant soil carbon sequestration was observed over the year of conversion from conventional to no-till practices, with a mean rate of 0.41 Mg C ha(-1) year(-1). The same effect was observed in sugarcane fields, but with a much higher accumulation of carbon in soil stocks, when sugarcane fields are converted from burned to mechanised based harvest, where large amounts of sugarcane residues remain on the soil surface (1.8 Mg C ha(-1) year(-1)). The higher sequestration potential of sugarcane crops, when compared to the others, has a direct relation to the primary production of this crop. Nevertheless, much of this mitigation potential of soil carbon accumulation in sugarcane fields is lost once areas are reformed, or intensive tillage is applied. Pasture lands have shown soil carbon depletion once natural areas are converted to livestock use, while integration of those areas with agriculture use has shown an improvement in soil carbon stocks. Those works have shown that the main crop systems of Brazil have a huge mitigation potential, especially in soil carbon form, being an opportunity for future mitigation strategies. PMID:23011303

  12. Toxicological assessment of heavy metals accumulated in vegetables and fruits grown in Ginfel river near Sheba Tannery, Tigray, Northern Ethiopia.

    PubMed

    Gebrekidan, Abraha; Weldegebriel, Yirgaalem; Hadera, Amanual; Van der Bruggen, Bart

    2013-09-01

    The accumulation of heavy metals in vegetables resulting from irrigation with contaminated water obtained from industrial effluents may create a potential public health risk. We quantified the concentration of heavy metals (Cu, Zn, Fe, Mn, Cr, Cd, Ni, Co and Pb) in soil, vegetables and the water used for irrigation at two sites (Laelay Wukro and Tahtay Wukro) around Wukro Town, Tigray, Northern Ethiopia. The concentrations of heavy metals in irrigation water measured during this study were lower than permissible limits of heavy metals allowed for irrigation water. The mean concentrations of heavy metals in irrigated soil samples obtained from Tahtay Wukro were higher for Mn, Zn, Cr, and Cu. The overall results of soil samples ranged 2.62-827, 1.4-51.6, 25.5-33.6, 23.5-28.2, 2.52-25.1, 15-17.8, 3-4, 2.5-40.49 and 0.7-0.8 mg/kg for Mn, Zn, Cr, Ni, Cu, Co, Pb, Fe and Cd, respectively. Higher concentrations of heavy metals were also observed in vegetable samples from Tahtay Wukro. Pb was found to accumulate the most in all vegetable samples. It was observed that green pepper and lettuce accumulate high amounts of Cu and Zn; Swiss chard accumulates excessive amounts of Fe, Mn, Cr, Cd, Ni and Co; lettuce and tomato higher amounts of Cd; and green pepper, tomato and onion a higher concentration of Pb. Significant differences in the elemental concentrations between the vegetables analyzed from Laelay and Tahtay Wukro were observed. This was attributed in part to the geological nature of the study area and the discharges from the town and from a tannery. The results also indicate that Fe, Pb and Cd have high transfer factor values (mean values: 42.89, 0.84 and 0.37, respectively). The transfer pattern for heavy metals in different vegetables showed a trend in the order: Fe>Pb>Cd>Mn>Cu>Zn>Ni>Zn>Cr=Co. The heavy metal contamination of vegetables grown in Tahtay Wukro, located downstream of the tannery, may pose increased health risks in the future to the local population

  13. Geographic variability in organic carbon stock and accumulation rate in sediments of East and Southeast Asian seagrass meadows

    NASA Astrophysics Data System (ADS)

    Miyajima, Toshihiro; Hori, Masakazu; Hamaguchi, Masami; Shimabukuro, Hiromori; Adachi, Hiroshi; Yamano, Hiroya; Nakaoka, Masahiro

    2015-04-01

    Organic carbon (OC) stored in the sediments of seagrass meadows has been considered a globally significant OC reservoir. However, the sparsity and regional bias of studies on long-term OC accumulation in coastal sediments have limited reliable estimation of the capacity of seagrass meadows as a global OC sink. We evaluated the amount and accumulation rate of OC in sediment of seagrass meadows and adjacent areas in East and Southeast Asia. In temperate sites, the average OC concentration in the top 30 cm of sediment was higher in seagrass meadows (780-1080 mmol g-1) than in sediments without seagrass cover (52-430 mmol g-1). The average OC in the top 30 cm of subtropical and tropical seagrass meadow sediments ranged from 140 to 440 mmol g-1. Carbon isotope mass balancing suggested that the contribution of seagrass-derived carbon to OC stored in sediments was often relatively minor (temperate: 10-40%; subtropical: 35-82%; tropical: 4-34%) and correlated to the habitat type, being particularly low in estuarine habitats. Stock of OC in the top meter of sediment of all the studied meadows ranged from 38 to 120 Mg ha-1. The sediment accumulation rates were estimated by radiocarbon dating of six selected cores (0.32-1.34 mm yr-1). The long-term OC accumulation rates calculated from the sediment accumulation rate and the top 30 cm average OC concentration for the seagrass meadows (24-101 kg ha-1 yr-1) were considerably lower than the OC accumulation rates previously reported for Mediterranean Posidonia oceanica meadows (580 kg ha-1 yr-1 on average). Current estimates for the global carbon sink capacity of seagrass meadows, which rely largely on Mediterranean studies, may be considerable overestimations.

  14. Maximum likelihood decoding analysis of Accumulate-Repeat-Accumulate Codes

    NASA Technical Reports Server (NTRS)

    Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

    2004-01-01

    Repeat-Accumulate (RA) codes are the simplest turbo-like codes that achieve good performance. However, they cannot compete with Turbo codes or low-density parity check codes (LDPC) as far as performance is concerned. The Accumulate Repeat Accumulate (ARA) codes, as a subclass of LDPC codes, are obtained by adding a pre-coder in front of RA codes with puncturing where an accumulator is chosen as a precoder. These codes not only are very simple, but also achieve excellent performance with iterative decoding. In this paper, the performance of these codes with (ML) decoding are analyzed and compared to random codes by very tight bounds. The weight distribution of some simple ARA codes is obtained, and through existing tightest bounds we have shown the ML SNR threshold of ARA codes approaches very closely to the performance of random codes. We have shown that the use of precoder improves the SNR threshold but interleaving gain remains unchanged with respect to RA code with puncturing.

  15. N₂O accumulation from denitrification under different temperatures.

    PubMed

    Poh, Leong Soon; Jiang, Xie; Zhang, Zhongbo; Liu, Yu; Ng, Wun Jern; Zhou, Yan

    2015-11-01

    The effects of temperature on nitrous oxide (N2O) accumulation during denitrification and denitritation were investigated. Batch experiments were performed to measure N2O accumulation at 25 and 35 °C. More N2O accumulation was observed during denitritation at the higher temperature as compared with full denitrification and low temperature tests. The highest nitrite concentration tested in this study (25 mg/L NO2 (-)N and pH 8.0) did not show inhibitory effect on N2O reduction. It was found that the major cause of more N2O accumulation during denitrification at higher temperature was due to higher N2O production rate and lower N2O solubility. Specific nitrate, nitrite, and N2O reduction rates increased 62, 61, and 41 %, respectively, when temperature rose from 25 to 35 °C. The decrease of N2O solubility in mixed liquor at 35 °C (when compared to 25 °C) resulted in faster diffusing rate of N2O from liquid to gas phase. It was also more difficult for gas phase N2O to be re-dissolved. The diffused N2O was then accumulated in the headspace, which was not available for denitrification by denitrifiers. The results of this study suggest higher temperature may worsen N2O emission from wastewater treatment plants (WWTPs). PMID:26129949

  16. 75 FR 58407 - Medicare Program; Medicare Appeals; Adjustment to the Amount in Controversy Threshold Amounts for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-24

    ... for requests for ALJ hearings and judicial review filed on or after January 1, 2011. The 2011 AIC threshold amounts are $130 for ALJ hearings and $1,300 for judicial review. DATES: Effective Date: This... and judicial review at $100 and $1,000, respectively, for Medicare Part A and Part B appeals....

  17. Different approaches for improving skin accumulation of topical corticosteroids.

    PubMed

    Senyiğit, Taner; Padula, Cristina; Ozer, Ozgen; Santi, Patrizia

    2009-10-01

    The aim of this paper was to evaluate the effect of vehicle, chemical enhancer and iontophoresis on the skin accumulation of clobetasol propionate (CP) and mometasone furoate (MF). In vitro permeation experiments were performed using pig ear skin as barrier and HPLC as quantification method. The formulations tested were chitosan gels, sodium-deoxycholate gels and commercial creams of CP and MF. The results obtained indicate that Na-DOC gel had an enhancing effect on the skin accumulation of both active agents. This effect was more evident with CP especially in the stratum corneum and epidermis which are the target sites of topical steroidal treatment. Two terpene derivatives (D-limonene and nerolidol) and Transcutol P were evaluated as chemical penetration enhancers. Nerolidol produced considerable increase in the amount of CP and MF accumulated without any permeation across the skin. The application of electric current (anodal iontophoresis) to the gels improved the accumulation of MF while it did not effect the accumulation of CP. Due to the best accumulation results of nerolidol, the enhancement effect in combination with iontophoresis was also investigated. It was shown that, the combination of anodal iontophoresis and chemical enhancer (nerolidol) produced no further enhancement for both active agents. PMID:19635541

  18. Bubble accumulation and its role in the evolution of magma reservoirs in the upper crust.

    PubMed

    Parmigiani, A; Faroughi, S; Huber, C; Bachmann, O; Su, Y

    2016-04-28

    Volcanic eruptions transfer huge amounts of gas to the atmosphere. In particular, the sulfur released during large silicic explosive eruptions can induce global cooling. A fundamental goal in volcanology, therefore, is to assess the potential for eruption of the large volumes of crystal-poor, silicic magma that are stored at shallow depths in the crust, and to obtain theoretical bounds for the amount of volatiles that can be released during these eruptions. It is puzzling that highly evolved, crystal-poor silicic magmas are more likely to generate volcanic rocks than plutonic rocks. This observation suggests that such magmas are more prone to erupting than are their crystal-rich counterparts. Moreover, well studied examples of largely crystal-poor eruptions (for example, Katmai, Taupo and Minoan) often exhibit a release of sulfur that is 10 to 20 times higher than the amount of sulfur estimated to be stored in the melt. Here we argue that these two observations rest on how the magmatic volatile phase (MVP) behaves as it rises buoyantly in zoned magma reservoirs. By investigating the fluid dynamics that controls the transport of the MVP in crystal-rich and crystal-poor magmas, we show how the interplay between capillary stresses and the viscosity contrast between the MVP and the host melt results in a counterintuitive dynamics, whereby the MVP tends to migrate efficiently in crystal-rich parts of a magma reservoir and accumulate in crystal-poor regions. The accumulation of low-density bubbles of MVP in crystal-poor magmas has implications for the eruptive potential of such magmas, and is the likely source of the excess sulfur released during explosive eruptions. PMID:27074507

  19. Bubble accumulation and its role in the evolution of magma reservoirs in the upper crust

    NASA Astrophysics Data System (ADS)

    Parmigiani, A.; Faroughi, S.; Huber, C.; Bachmann, O.; Su, Y.

    2016-04-01

    Volcanic eruptions transfer huge amounts of gas to the atmosphere. In particular, the sulfur released during large silicic explosive eruptions can induce global cooling. A fundamental goal in volcanology, therefore, is to assess the potential for eruption of the large volumes of crystal-poor, silicic magma that are stored at shallow depths in the crust, and to obtain theoretical bounds for the amount of volatiles that can be released during these eruptions. It is puzzling that highly evolved, crystal-poor silicic magmas are more likely to generate volcanic rocks than plutonic rocks. This observation suggests that such magmas are more prone to erupting than are their crystal-rich counterparts. Moreover, well studied examples of largely crystal-poor eruptions (for example, Katmai, Taupo and Minoan) often exhibit a release of sulfur that is 10 to 20 times higher than the amount of sulfur estimated to be stored in the melt. Here we argue that these two observations rest on how the magmatic volatile phase (MVP) behaves as it rises buoyantly in zoned magma reservoirs. By investigating the fluid dynamics that controls the transport of the MVP in crystal-rich and crystal-poor magmas, we show how the interplay between capillary stresses and the viscosity contrast between the MVP and the host melt results in a counterintuitive dynamics, whereby the MVP tends to migrate efficiently in crystal-rich parts of a magma reservoir and accumulate in crystal-poor regions. The accumulation of low-density bubbles of MVP in crystal-poor magmas has implications for the eruptive potential of such magmas, and is the likely source of the excess sulfur released during explosive eruptions.

  20. Accumulation of indole alkaloids in a suspension culture of Tabernaemontana divaricata.

    PubMed

    van der Heijden, R; Hermans-Lokkerbol, A; de Kool, L P; Lamping, P J; Harkes, P A; Verpoorte, R

    1988-10-01

    Cell suspension cultures of TABERNAEMONTANA DIVARICATA were found to produce relatively large amounts of indole alkaloids. For their isolation an ion-pair DCCC method was used in combination with preparative TLC. The alkaloids were identified as tabernaemontanine, perivine, vobasine, voaphylline, voaphylline-hydroxyindolenine, vallesamine, apparicine, 16-hydroxy-16,22-dihydroapparicine, pericyclivine, tubotaiwine, 19- S-heyneanine, and coronaridine. Voaphylline, the main alkaloid, was produced during growth and early stationary phase and reached a maximum of 23 mg/l at day 19 of the growth cycle. After this maximum voaphylline was rapidly metabolized. Apparicine, vobasine, and coronaridine reached their maximum levels at a later stage of the growth cycle. Tubotaiwine accumulation showed a similar profile as that of voaphylline. In light-grown cells the total production was about 2 times higher than in dark-grown cells, with respective main products voaphylline and apparicine. PMID:17265301

  1. Photosynthetic light reactions increase total lipid accumulation in carbon-supplemented batch cultures of Chlorella vulgaris.

    PubMed

    Woodworth, Benjamin D; Mead, Rebecca L; Nichols, Courtney N; Kolling, Derrick R J

    2015-03-01

    Microalgae are an attractive biofuel feedstock because of their high lipid to biomass ratios, lipid compositions that are suitable for biodiesel production, and the ability to grow on varied carbon sources. While algae can grow autotrophically, supplying an exogenous carbon source can increase growth rates and allow heterotrophic growth in the absence of light. Time course analyses of dextrose-supplemented Chlorella vulgaris batch cultures demonstrate that light availability directly influences growth rate, chlorophyll production, and total lipid accumulation. Parallel photomixotrophic and heterotrophic cultures grown to stationary phase reached the same amount of biomass, but total lipid content was higher for algae grown in the presence of light (an average of 1.90 mg/mL vs. 0.77 mg/mL over 5 days of stationary phase growth). PMID:25543540

  2. Accumulation of uranium derived from long-term fertilizer applications in a cultivated Andisol.

    PubMed

    Takeda, Akira; Tsukada, Hirofumi; Takaku, Yuichi; Hisamatsu, Shun'ichi; Nanzyo, Masami

    2006-08-31

    The U concentrations in the soils of experimental fields with continuous fertilizer applications and in neighboring non-agricultural soils were determined. The surface soils in the three experimental fields with fertilizer applications contained higher amounts of U compared with the non-agricultural surface soils. The amount of U elevated in the soil was estimated by the vertical profile of U concentration, and an increase of about 200 mg m-2 of U was found in the soils at 0-35 cm depth during a 61-year cultivation period. The estimated value was almost the same as the amount of U added through the fertilizers as calculated from U concentrations in the applied fertilizers. Therefore, almost all the U from the fertilizers would still remain in the upper part of the soils. Chemical extraction results suggested that organic substances and noncrystalline clay minerals in the surface soil should play an important role for accumulation of U derived from the fertilizers. PMID:16487995

  3. Accumulation and depuration of cyanobacterial paralytic shellfish toxins by the freshwater mussel Anodonta cygnea.

    PubMed

    Pereira, Paulo; Dias, Elsa; Franca, Susana; Pereira, Elisa; Carolino, Manuela; Vasconcelos, Vitor

    2004-07-14

    The increasing frequency by which the production of paralytic shellfish toxins (PST) by freshwater bloom-forming cyanobacteria is being noticed world-wide raises the possibility of PST bioaccumulation by freshwater mussels. This study evaluates PST accumulation and depuration by the freshwater mussel Anodonta cygnea exposed over a 14-day period to high densities (mean = 1.4 x 10(9) cells1(-1), S.D. = 0.29 x 10(9) cellsl(-1)) of the toxic cyanobacterium Aphanizomenon issatschenkoi (corresponding to a mean toxin concentration of 25.5 nmol PSTl(-1), S.D. = 9.9 nmol PSTl(-1)). Mussels were subsequently detoxified either by starvation or by feeding on the non-toxic green-algae Ankistodesmus falcatus. Filter feeding activity and toxin uptake by the mussels were followed by cell counting and toxin analysis in water samples taken before and after each daily water renewal. The accumulation and depuration of PST as well as the anatomical distribution of toxins were monitored throughout the experiment by HPLC analysis of mussel extracts. Mussels fed the toxic cyanobacterium removed on average 65.3% of cells and 40.36% of total PST daily provided. Daily rates of cell clearance (% of initial) were negatively correlated with the amounts of PST daily provided (but not with the amount of cells). This suggests a negative effect of toxins on the feeding behaviour of mussels. Small amounts of toxins could be detected in the mussels after the second day of exposure, reaching a maximum of 26 microg PST100 g(-1) by day 7. The viscera contained the greatest proportion of toxins (78%) at the start of the toxification. However, increasing amounts of PST were found in the remaining tissues (gills, mantle and foot) over time. Toxins detected in the mussel extracts were the same provided in the dietary A. issatschenkoi. Nevertheless, mussels showed a higher proportion of saxitoxin and decarbomoylsaxitoxin and a lower proportion of gonyautoxin-5 than the fed cyanobacterium. Similar depuration

  4. Ecology: accumulating threats to life

    SciTech Connect

    Peterson, R.W.

    1980-04-01

    The accumulating impacts of toxic materials like polychloridnated bephenyls (PCBs), acid rain, deforestation in the Amazon River Basin, and nuclear energy are examined as life-threatening actions that the public must recognize. Immediate action is needed to abandon destructive human activities and search out those life-supporting choices which will replace immediate gratification with long-range benefits. (DCK)

  5. Structural evaluation of phospholipidic nanovesicles containing small amounts of chitosan.

    PubMed

    Mertins, Omar; Cardoso, Mateus Borba; Pohlmann, Adriana Raffin; da Silveira, Nádya Pesce

    2006-08-01

    In this study we present a full characterization of nanovesicles containing soybean phosphatidylcholine and polysaccharide chitosan. The nanovesicles were prepared by the reverse phase evaporation method, including the preparation of reverse micelles followed by the formation of an organogel, which is dispersed in water to yield the final liposomal particles. Structural changes as a function of the chitosan amount and the filter porosity used in the nanovesicles preparation were studied employing Static and Dynamic Light Scattering as well as Small Angle X-ray Scattering. The hydrodynamic radius of the nanovesicles ranged between 106 and 287 nm, depending on the chitosan contents and the filter porosity. A comparison with nanovesicles free of chitosan indicates the existence of higher contents of multilamellar structures that depends on the chitosan concentration in the vesicles containing chitosan. Typical spherical vesicles having nanometric diameters with polydispersity mostly desired in the biomedical area could only be achieved by filtration through a 0.45 microm porous filter. PMID:17037851

  6. Gas hydrate accumulation at the Hakon Mosby Mud Volcano

    USGS Publications Warehouse

    Ginsburg, G.D.; Milkov, A.V.; Soloviev, V.A.; Egorov, A.V.; Cherkashev, G.A.; Vogt, P.R.; Crane, K.; Lorenson, T.D.; Khutorskoy, M.D.

    1999-01-01

    Gas hydrate (GH) accumulation is characterized and modeled for the Hakon Mosby mud volcano, ca. 1.5 km across, located on the Norway-Barents-Svalbard margin. Pore water chemical and isotopic results based on shallow sediment cores as well as geothermal and geomorphological data suggest that the GH accumulation is of a concentric pattern controlled by and formed essentially from the ascending mud volcano fluid. The gas hydrate content of sediment peaks at 25% by volume, averaging about 1.2% throughout the accumulation. The amount of hydrate methane is estimated at ca. 108 m3 STP, which could account for about 1-10% of the gas that has escaped from the volcano since its origin.

  7. The Comparative Study of Element Accumulation in Wood Fen Peat (Latvia)

    NASA Astrophysics Data System (ADS)

    Krumins, Janis; Klavins, Maris; Kuske, Eliza; Seglins, Valdis; Kaup, Enn

    2013-04-01

    Mires belong to the most representative archives of past environmental conditions in large areas of temperate and subarctic zone. Moreover, mires keep evidence of ancient cultures and modern human activity. Consequently the research of mires is an integral part of global change studies. Fens are less studied than bogs; one of the reasons is the complexity of factors that impact peat formation. Bogs, due to dome-shaped structure, are affected by precipitation, while other external influences are negligible and simply separable. The aim of this research was the characterization of accumulation patterns of metallic elements in wood fen peat profiles and to assess their accumulation regularities in relation with peat properties. The general idea was to find out how admixtures of plant remains in different stages of decomposition change properties and the element accumulation character in a wood peat. In obtained profiles were separated five types of wood peat: wood, wood-sedge, wood-reed, wood-grass and wood- sphagnum peat. Peat was sampled in four Latvian fens: Elki, Viki, Svetupe and Sala. Similar environment, origin and development of sites suggest similar development of peat properties thus there is no reason to assume different impact on peat development among mires. Despite a slow decomposition rate, results point to a higher decomposition degree of wood peat, in comparison with other types of fen peat. In average, wood peat forms the thickest layers, but it must be taken into account that thickness depends on coating layers, presence of decomposed plant remains etc. The accumulation pattern of metallic elements in a wood fen peat slightly differs among sites, but the difference among wood peat types is clearly evident. For instance, the highest amount of iron is characteristic to wood-reed peat, while, the lowest, is in wood-sphagnum peat. At the same time, in wood-reed peat the lowest amounts of magnesium were found while the highest amount of Mg was in wood

  8. Survival strategies of polyphosphate accumulating organisms and glycogen accumulating organisms under conditions of low organic loading.

    PubMed

    Carvalheira, Mónica; Oehmen, Adrian; Carvalho, Gilda; Reis, Maria A M

    2014-11-01

    Enhanced biological phosphorus removal (EBPR) is usually limited by organic carbon availability in wastewater treatment plants (WWTPs). Polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) were operated under extended periods with low organic carbon loading in order to examine its impact on their activity and survival. The decrease in organic carbon load affected PAOs and GAOs in different ways, where the biomass decay rate of GAOs was approximately 4times higher than PAOs. PAOs tended to conserve a relatively high residual concentration of polyhydroxyalkanoates (PHAs) under aerobic conditions, while GAOs tended to deplete their available PHA more rapidly. This slower oxidation rate of PHA by PAOs at residual concentration levels enabled them to maintain an energy source for aerobic maintenance processes for longer than GAOs. This may provide PAOs with an advantage over GAOs in surviving the low organic loading conditions commonly found in full-scale wastewater treatment plants. PMID:25270044

  9. Contrast-enhanced micro-computed tomography of fatigue microdamage accumulation in human cortical bone.

    PubMed

    Landrigan, Matthew D; Li, Jiliang; Turnbull, Travis L; Burr, David B; Niebur, Glen L; Roeder, Ryan K

    2011-03-01

    Conventional methods used to image and quantify microdamage accumulation in bone are limited to histological sections, which are inherently invasive, destructive, two-dimensional, and tedious. These limitations inhibit investigation of microdamage accumulation with respect to volumetric spatial variation in mechanical loading, bone mineral density, and microarchitecture. Therefore, the objective of this study was to investigate non-destructive, three-dimensional (3-D) detection of microdamage accumulation in human cortical bone using contrast-enhanced micro-computed tomography (micro-CT), and to validate micro-CT measurements against conventional histological methods. Unloaded controls and specimens loaded in cyclic uniaxial tension to a 5% and 10% reduction in secant modulus were labeled with a precipitated BaSO₄ stain for micro-CT and basic fuchsin for histomorphometry. Linear microcracks were similarly labeled by BaSO₄ and basic fuchsin as shown by backscattered electron microscopy and light microscopy, respectively. The higher X-ray attenuation of BaSO₄ relative to the bone extracellular matrix provided enhanced contrast for the detection of damage that was otherwise not able to be detected by micro-CT prior to staining. Therefore, contrast-enhanced micro-CT was able to nondestructively detect the presence, 3-D spatial location, and accumulation of fatigue microdamage in human cortical bone specimens in vitro. Microdamage accumulation was quantified on segmented micro-CT reconstructions as the ratio of BaSO₄ stain volume (SV) to total bone volume (BV). The amount of microdamage measured by both micro-CT (SV/BV) and histomorphometry (Cr.N, Cr.Dn, Cr.S.Dn) progressively increased from unloaded controls to specimens loaded to a 5% and 10% reduction in secant modulus (p < 0.001). Group means for micro-CT measurements of damage accumulation were strongly correlated to those using histomorphometry (p < 0.05), validating the new methods. Limitations of the new

  10. 20 CFR 340.13 - Compromise of amounts recoverable.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... INSURANCE ACT RECOVERY OF BENEFITS § 340.13 Compromise of amounts recoverable. The Board or its designee may compromise an amount recoverable, provided such amount does not exceed $100,000, excluding interest, or such... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Compromise of amounts recoverable....

  11. 20 CFR 416.503 - Minimum monthly benefit amount.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... monthly benefit amount. If you receive an SSI benefit that does not include a State supplement the minimum monthly SSI benefit amount payable is $1. When an SSI benefit amount of less than $1 is payable, the benefit amount will be increased to $1. If you receive an SSI benefit that does include a State...

  12. 42 CFR 418.309 - Hospice cap amount.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 3 2010-10-01 2010-10-01 false Hospice cap amount. 418.309 Section 418.309 Public...) MEDICARE PROGRAM HOSPICE CARE Payment for Hospice Care § 418.309 Hospice cap amount. The hospice cap amount... until October 31 of the following year. (b) Each hospice's cap amount is calculated by the...

  13. 20 CFR 234.46 - Amount of the RLS payable.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 1 2011-04-01 2011-04-01 false Amount of the RLS payable. 234.46 Section 234...-SUM PAYMENTS Residual Lump-Sum Payment § 234.46 Amount of the RLS payable. The gross RLS amount is... this chapter, respectively.) The amount of the RLS payable is equal to the gross RLS minus the sum...

  14. 20 CFR 234.46 - Amount of the RLS payable.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 1 2014-04-01 2012-04-01 true Amount of the RLS payable. 234.46 Section 234...-SUM PAYMENTS Residual Lump-Sum Payment § 234.46 Amount of the RLS payable. The gross RLS amount is... this chapter, respectively.) The amount of the RLS payable is equal to the gross RLS minus the sum...

  15. 20 CFR 234.46 - Amount of the RLS payable.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 1 2013-04-01 2012-04-01 true Amount of the RLS payable. 234.46 Section 234...-SUM PAYMENTS Residual Lump-Sum Payment § 234.46 Amount of the RLS payable. The gross RLS amount is... this chapter, respectively.) The amount of the RLS payable is equal to the gross RLS minus the sum...

  16. 20 CFR 234.46 - Amount of the RLS payable.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 1 2012-04-01 2012-04-01 false Amount of the RLS payable. 234.46 Section 234...-SUM PAYMENTS Residual Lump-Sum Payment § 234.46 Amount of the RLS payable. The gross RLS amount is... this chapter, respectively.) The amount of the RLS payable is equal to the gross RLS minus the sum...

  17. 20 CFR 234.46 - Amount of the RLS payable.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false Amount of the RLS payable. 234.46 Section 234...-SUM PAYMENTS Residual Lump-Sum Payment § 234.46 Amount of the RLS payable. The gross RLS amount is... this chapter, respectively.) The amount of the RLS payable is equal to the gross RLS minus the sum...

  18. 46 CFR 58.30-25 - Accumulators.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... RELATED SYSTEMS Fluid Power and Control Systems § 58.30-25 Accumulators. (a) An accumulator is an unfired... fluid. Accumulators must meet the applicable requirements in § 54.01-5 (c)(3), (c)(4), and (d) of this chapter or the remaining requirements in part 54. (b) If the accumulator is of the gas and fluid...

  19. 46 CFR 58.30-25 - Accumulators.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... RELATED SYSTEMS Fluid Power and Control Systems § 58.30-25 Accumulators. (a) An accumulator is an unfired... fluid. Accumulators must meet the applicable requirements in § 54.01-5 (c)(3), (c)(4), and (d) of this chapter or the remaining requirements in part 54. (b) If the accumulator is of the gas and fluid...

  20. 46 CFR 58.30-25 - Accumulators.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... RELATED SYSTEMS Fluid Power and Control Systems § 58.30-25 Accumulators. (a) An accumulator is an unfired... fluid. Accumulators must meet the applicable requirements in § 54.01-5 (c)(3), (c)(4), and (d) of this chapter or the remaining requirements in part 54. (b) If the accumulator is of the gas and fluid...

  1. 46 CFR 58.30-25 - Accumulators.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... RELATED SYSTEMS Fluid Power and Control Systems § 58.30-25 Accumulators. (a) An accumulator is an unfired... fluid. Accumulators must meet the applicable requirements in § 54.01-5 (c)(3), (c)(4), and (d) of this chapter or the remaining requirements in part 54. (b) If the accumulator is of the gas and fluid...

  2. Metabolic engineering of higher plants and algae for isoprenoid production.

    PubMed

    Kempinski, Chase; Jiang, Zuodong; Bell, Stephen; Chappell, Joe

    2015-01-01

    Isoprenoids are a class of compounds derived from the five carbon precursors, dimethylallyl diphosphate, and isopentenyl diphosphate. These molecules present incredible natural chemical diversity, which can be valuable for humans in many aspects such as cosmetics, agriculture, and medicine. However, many terpenoids are only produced in small quantities by their natural hosts and can be difficult to generate synthetically. Therefore, much interest and effort has been directed toward capturing the genetic blueprint for their biochemistry and engineering it into alternative hosts such as plants and algae. These autotrophic organisms are attractive when compared to traditional microbial platforms because of their ability to utilize atmospheric CO2 as a carbon substrate instead of supplied carbon sources like glucose. This chapter will summarize important techniques and strategies for engineering the accumulation of isoprenoid metabolites into higher plants and algae by choosing the correct host, avoiding endogenous regulatory mechanisms, and optimizing potential flux into the target compound. Future endeavors will build on these efforts by fine-tuning product accumulation levels via the vast amount of available "-omic" data and devising metabolic engineering schemes that integrate this into a whole-organism approach. With the development of high-throughput transformation protocols and synthetic biology molecular tools, we have only begun to harness the power and utility of plant and algae metabolic engineering. PMID:25636485

  3. CONCEPTUAL MODEL FOR ORIGIN OF ABNORMALLY PRESSURED GAS ACCUMULATIONS IN LOW-PERMEABILITY RESERVOIRS.

    USGS Publications Warehouse

    Law, B.E.; Dickinson, W.W.

    1985-01-01

    The paper suggests that overpressured and underpressured gas accumulations of this type have a common origin. In basins containing overpressured gas accumulations, rates of thermogenic gas accumulation exceed gas loss, causing fluid (gas) pressure to rise above the regional hydrostatic pressure. Free water in the larger pores is forced out of the gas generation zone into overlying and updip, normally pressured, water-bearing rocks. While other diagenetic processes continue, a pore network with very low permeability develops. As a result, gas accumulates in these low-permeability reservoirs at rates higher than it is lost. In basins containing underpressured gas accumulations, rates of gas generation and accumulation are less than gas loss. The basin-center gas accumulation persists, but because of changes in the basin dynamics, the overpressured accumulation evolves into an underpressured system.

  4. Metal accumulating plants: Medium's role

    NASA Astrophysics Data System (ADS)

    Rabier, J.; Prudent, P.; Szymanska, B.; Mevy, J.-P.

    2003-05-01

    To evaluate phytoremediation potentialities by metal accumulation in tolerant plants, trials are carried out using in vitro cultures. Organie compounds influence on metal accumulation is studied with metals supplemented media. The tested compounds on zinc and lead absorption by Brassica juncea, are chelating agents (EDTA, citric acid) and soluble organic fractions of compost. EDTA seems to enhance the transfer of lead in plant but it is the opposite in the case of zinc. Citric acid stimulates root absorption for both zinc and lead. For the aqueous extracts of compost, variable effects are obtained according to the origin of compost (green wastes and food wastes). In'all tested conditions of cultures, zinc is mainly exported towards shoot while lead is stored in root.

  5. Heat accumulation during sequential cortical bone drilling.

    PubMed

    Palmisano, Andrew C; Tai, Bruce L; Belmont, Barry; Irwin, Todd A; Shih, Albert; Holmes, James R

    2016-03-01

    Significant research exists regarding heat production during single-hole bone drilling. No published data exist regarding repetitive sequential drilling. This study elucidates the phenomenon of heat accumulation for sequential drilling with both Kirschner wires (K wires) and standard two-flute twist drills. It was hypothesized that cumulative heat would result in a higher temperature with each subsequent drill pass. Nine holes in a 3 × 3 array were drilled sequentially on moistened cadaveric tibia bone kept at body temperature (about 37 °C). Four thermocouples were placed at the center of four adjacent holes and 2 mm below the surface. A battery-driven hand drill guided by a servo-controlled motion system was used. Six samples were drilled with each tool (2.0 mm K wire and 2.0 and 2.5 mm standard drills). K wire drilling increased temperature from 5 °C at the first hole to 20 °C at holes 6 through 9. A similar trend was found in standard drills with less significant increments. The maximum temperatures of both tools increased from <0.5 °C to nearly 13 °C. The difference between drill sizes was found to be insignificant (P > 0.05). In conclusion, heat accumulated during sequential drilling, with size difference being insignificant. K wire produced more heat than its twist-drill counterparts. This study has demonstrated the heat accumulation phenomenon and its significant effect on temperature. Maximizing the drilling field and reducing the number of drill passes may decrease bone injury. PMID:26334198

  6. Mechanisms of intrahepatic triglyceride accumulation

    PubMed Central

    Ress, Claudia; Kaser, Susanne

    2016-01-01

    Hepatic steatosis defined as lipid accumulation in hepatocytes is very frequently found in adults and obese adolescents in the Western World. Etiologically, obesity and associated insulin resistance or excess alcohol intake are the most frequent causes of hepatic steatosis. However, steatosis also often occurs with chronic hepatitis C virus (HCV) infection and is also found in rare but potentially life-threatening liver diseases of pregnancy. Clinical significance and outcome of hepatic triglyceride accumulation are highly dependent on etiology and histological pattern of steatosis. This review summarizes current concepts of pathophysiology of common causes of hepatic steatosis, including non-alcoholic fatty liver disease (NAFLD), alcoholic fatty liver disease, chronic HCV infections, drug-induced forms of hepatic steatosis, and acute fatty liver of pregnancy. Regarding the pathophysiology of NAFLD, this work focuses on the close correlation between insulin resistance and hepatic triglyceride accumulation, highlighting the potential harmful effects of systemic insulin resistance on hepatic metabolism of fatty acids on the one side and the role of lipid intermediates on insulin signalling on the other side. Current studies on lipid droplet morphogenesis have identified novel candidate proteins and enzymes in NAFLD. PMID:26819531

  7. Abundance and characteristics of microplastics in beach sediments: Insights into microplastic accumulation in northern Gulf of Mexico estuaries.

    PubMed

    Wessel, Caitlin C; Lockridge, Grant R; Battiste, David; Cebrian, Just

    2016-08-15

    Microplastics (plastic debris smaller than 5mm) represent a growing concern worldwide due to increasing amounts of discarded trash. We investigated microplastic debris on sandy shorelines at seven locations in a northern Gulf of Mexico estuary (Mobile Bay, AL) during the summer of 2014. Microplastics were ubiquitous throughout the area studied at concentrations 66-253× larger than reported for the open ocean. The polymers polypropylene and polyethylene were most abundant, with polystyrene, polyester and aliphatic polyamide also present but in lower quantities. There was a gradient in microplastic abundance, with locations more directly exposed to marine currents and tides having higher microplastic abundance and diversity, as well as a higher contribution by denser polymers (e.g. polyester). These results indicate that microplastic accumulation on shorelines in the northern Gulf of Mexico may be a serious concern, and suggest that exposure to inputs from the Gulf is an important determinant of microplastic abundance. PMID:27287867

  8. Yeast Extract and Silver Nitrate Induce the Expression of Phenylpropanoid Biosynthetic Genes and Induce the Accumulation of Rosmarinic Acid in Agastache rugosa Cell Culture.

    PubMed

    Park, Woo Tae; Arasu, Mariadhas Valan; Al-Dhabi, Naif Abdullah; Yeo, Sun Kyung; Jeon, Jin; Park, Jong Seok; Lee, Sook Young; Park, Sang Un

    2016-01-01

    The present study aimed to investigate the role of yeast extract and silver nitrate on the enhancement of phenylpropanoid pathway genes and accumulation of rosmarinic acid in Agastache rugosa cell cultures. The treatment of cell cultures with yeast extract (500 mg/L) and silver nitrate (30 mg/L) for varying times enhanced the expression of genes in the phenylpropanoid pathway and the production of rosmarinic acid. The results indicated that the expression of RAS and HPPR was proportional to the amount of yeast extract and silver nitrate. The transcript levels of HPPR under yeast extract treatment were 1.84-, 1.97-, and 2.86-fold higher than the control treatments after 3, 6, and 12 h, respectively, whereas PAL expression under silver nitrate treatment was 52.31-fold higher than in the non-treated controls after 24 h of elicitation. The concentration of rosmarinic acid was directly proportional to the concentration of the applied elicitors. Yeast extract supplementation documented the highest amount of rosmarinic acid at 4.98 mg/g, whereas silver nitrate addition resulted in a comparatively lower amount of rosmarinic acid at 0.65 mg/g. In conclusion, addition of yeast extract to the cell cultures enhanced the accumulation of rosmarinic acid, which was evidenced by the expression levels of the phenylpropanoid biosynthetic pathway genes in A. rugosa. PMID:27043507

  9. 42 CFR 419.41 - Calculation of national beneficiary copayment amounts and national Medicare program payment amounts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... amounts and national Medicare program payment amounts. 419.41 Section 419.41 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM... Calculation of national beneficiary copayment amounts and national Medicare program payment amounts. (a)...

  10. 42 CFR 419.41 - Calculation of national beneficiary copayment amounts and national Medicare program payment amounts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... amounts and national Medicare program payment amounts. 419.41 Section 419.41 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM... Calculation of national beneficiary copayment amounts and national Medicare program payment amounts. (a)...

  11. Simulating inbreeding depression through the mutation accumulation theory

    NASA Astrophysics Data System (ADS)

    Sousa, A. O.; de Oliveira, S. Moss; Bernardes, Américo T.

    2000-04-01

    Using the Penna model for biological aging, which is based on the mutation accumulation theory, we show that the number of homozygous loci corresponding to deleterious mutations is higher in small populations than in large ones. This decrease of heterozygosity may drive small populations to extinction even when no drastic change of the environment occurs.

  12. Antiproton Accumulator in the Main Injector era (2)

    SciTech Connect

    Visnjic, V.

    1992-12-01

    By adding a single quadrupole per sextant in the Antiproton Accumulator it is possible to obtain a lattice well suited for higher bandwidth stochastic cooling systems such as those anticipated for the Main Injector era. The lattice proposed here has excellent properties concerning both the lattice functions and the stochastic cooling parameters.

  13. [Effects of maize plant types on dry matter accumulation characteristics and yield of soybean in maize-soybean intercropping systems].

    PubMed

    Cui, Liang; Yang, Wen-yu; Huang, Ni; Liu, Jiang; Wang, Yan-ling; Wang, Xiao-hui; Liu, Yang; Yan, Shou

    2015-08-01

    In order to explore the effects of maize plant types on dry matter accumulation and yield of soybean, a field experiment was conducted in 2013, including three maize-soybean relay strip intercropping systems. The relay strip intercropping systems were designed as soybean (Gongxuan 1) intercropped with Denghai 605 (RI1), Chuandan 418 (RI2) or Yayu 13 ( RI3), and the monocultured soybean was used as control. The results demonstrated that the dry matter accumulation rates of intercropped soybean in RI2 and RI3 treatments were lower than in RI1 treatment, and the leaf, stem and pod dry matter accumulation of intercropped soybean in RI1 treatment was 17.6%, 16.5% and 13.7% higher than that in RI2 treatment, and 34.6%, 33.1% and 28.4% higher than that in RI3 treatment, respectively. The distribution proportion of leaf and stem of intercropped soybean was in the order of RI1 > RI2 > RI3. However, the trend of the distribution proportion of pod was opposite. Compared with RI2 and RI3, the dry matter translocation amount, translocation proportion, contribution proportion of soybean vegetative organs to pod of soybean were improved in RI, treatment, and the pod per plant, seeds per plant, seeds per pod, yield per plant and yield of soybean in RI, were higher than RI2 and RI3 by 6.8%, 11.5%, 4.4%, 15.9%, 15.6% and 14.3%, 22.2%, 6.7%, 33.4%, 36.8%, respectively. The results showed that the yield was positively related with the accumulation rate of dry matter, dry matter translocation, dry matter translocation ratio and the contribution of dry matter accumulation, and these indices were highest in RI treatment. The results indicated that the compact maize relay intercropped with soybean could effectively regulate the dry matter accumulation, translocation and distribution, and improve the yield of soybean. PMID:26685605

  14. Adaptation strategies of two closely related Desmodesmus armatus (green alga) strains contained different amounts of cadmium: a study with light-induced synchronized cultures of algae.

    PubMed

    Pokora, Wojciech; Baścik-Remisiewicz, Agnieszka; Tukaj, Stefan; Kalinowska, Renata; Pawlik-Skowrońska, Barbara; Dziadziuszko, Małgorzata; Tukaj, Zbigniew

    2014-01-15

    During the Desmodesmus armatus cell cycle, 8-celled coenobia of 276-4d strain accumulated a much lower amounts of cadmium than unicells of B1-76 strain. Cadmium reduced growth and photosynthesis in the cells of strain B1-76, but not those of 276-4d strain. Cells of 276-4d strain revealed a higher activity of superoxide dismutase (SOD) isoforms, in particular the activity and protein content of Fe-SOD. Cu/Zn-SOD was earlier and much stronger induced by cadmium in 276-4d than in B1-76 strain, whereas Fe- and Mn-SOD activity and Fe-SOD synthesis were induced only in 276-4d strain. Cadmium did not affect the heat shock protein 70 synthesis in B1-76 strain, but significantly stimulated this process in 276-4d strain. The level of glutathione increased 30-fold during cell development of Cd-exposed 276-4d strain, while in B1-76 it increased about 12 timed. Matured cells of both strains exposed to cadmium produced comparable amounts of phytochelatins and other thiol peptides, but their production in young cells of B1-76 strain was much higher than in 276-4d strain. In conclusion, a complex of internal detoxification mechanisms appeared to be more efficient in cells of 276-4d strain than B1-76 one. PMID:24331421

  15. The Effects of Inorganic Nitrogen form and CO2 Concentration on Wheat Yield and Nutrient Accumulation and Distribution

    PubMed Central

    Carlisle, Eli; Myers, Samuel; Raboy, Victor; Bloom, Arnold

    2012-01-01

    Inorganic N is available to plants from the soil as ammonium (NH4+) and nitrate (NO3-). We studied how wheat grown hydroponically to senescence in controlled environmental chambers is affected by N form (NH4+ vs. NO3−) and CO2 concentration (“subambient,” “ambient,” and “elevated”) in terms of biomass, yield, and nutrient accumulation and partitioning. Wheat supplied with NH4+ as a sole N source had the strongest response to CO2 concentration. Plants exposed to subambient and ambient CO2 concentrations typically had the greatest biomass and nutrient accumulation under both N forms. In general NH4+-supplied plants had higher concentrations of total N, P, K, S, Ca, Zn, Fe, and Cu, while NO3--supplied plants had higher concentrations of Mg, B, Mn, and NO3- - N. NH4+-supplied plants contained amounts of phytate similar to NO3−-supplied plants but had higher bioavailable Zn, which could have consequences for human health. NH4+-supplied plants allocated more nutrients and biomass to aboveground tissues whereas NO3+-supplied plants allocated more nutrients to the roots. The two inorganic nitrogen forms influenced plant growth and nutrient status so distinctly that they should be treated as separate nutrients. Moreover, plant growth and nutrient status varied in a non-linear manner with atmospheric CO2 concentration. PMID:22969784

  16. A New Dynamic Accumulator for Batch Updates

    NASA Astrophysics Data System (ADS)

    Wang, Peishun; Wang, Huaxiong; Pieprzyk, Josef

    A dynamic accumulator is an algorithm, which gathers together a large set of elements into a constant-size value such that for a given element accumulated, there is a witness confirming that the element was indeed included into the value, with a property that accumulated elements can be dynamically added and deleted into/from the original set such that the cost of an addition or deletion operation is independent of the number of accumulated elements. Although the first accumulator was presented ten years ago, there is still no standard formal definition of accumulators. In this paper, we generalize formal definitions for accumulators, formulate a security game for dynamic accumulators so-called Chosen Element Attack (CEA), and propose a new dynamic accumulator for batch updates based on the Paillier cryptosystem. Our construction makes a batch of update operations at unit cost. We prove its security under the extended strong RSA (es-RSA) assumption.

  17. Different responses of low grain-Cd-accumulating and high grain-Cd-accumulating rice cultivars to Cd stress.

    PubMed

    Wang, Feijuan; Wang, Min; Liu, Zhouping; Shi, Yan; Han, Tiqian; Ye, Yaoyao; Gong, Ning; Sun, Junwei; Zhu, Cheng

    2015-11-01

    Cadmium (Cd) is a major heavy metal pollutant which is highly toxic to plants and animals. The accumulation of Cd in rice grains is a major agricultural problem in regions with Cd pollution. A hydroponics experiment using low grain-Cd-accumulating rice (xiushui 11) and high grain-Cd-accumulating rice (xiushui 110) was carried out to characterize the different responses of rice cultivars to Cd stress. We found that xiushui 11 was more tolerant to Cd than xiushui 110, and xiushui 11 suffered less oxidative damage. Cell walls played an important role in limiting the amount of Cd that entered the protoplast, especially in xiushui 11. Cd stored in organelles as soluble fractions, leading to greater physiological stress of Cd detoxification. We found that Cd can disturb the ion homeostasis in rice roots because Cd(2+) and Ca(2+) may have a similar uptake route. Xiushui 11 had a faster root-to-shoot transport of Cd, and the expression level of OsPCR1 gene which was predicted related with Cd accumulation in rice was consist with the Cd transport of root-to-shoot in rice and maintain the greater Cd tolerance of xiushui 11. These results suggest there are different Cd detoxification and accumulation mechanisms in rice cultivars. PMID:26318143

  18. Factors Affecting the Accumulation of Curcumin in Microrhizomes of Curcuma aromatica Salisb

    PubMed Central

    Zhang, Xiaoxia; Sun, Shulan; Wang, Xiaojing

    2015-01-01

    Curcuminoids, and mainly curcumin, are potential therapeutic agents for the prevention of various diseases; however, little is known about the factors that influence their accumulation in Curcuma species. In this study, the effects of factors such as sucrose concentration, different ratios of 6-benzylaminopurine (6-BA) and α-naphthalene acetic acid (NAA), and light quality on the accumulation of curcumin and other curcuminoids in Curcuma aromatica were investigated. Microrhizomes grown on media containing 3% sucrose produced more curcumin and other curcuminoids than those grown on higher concentrations. Moreover, when compared to other ratios of 6-BA and NAA, microrhizomes induced on 3% sucrose media supplemented with 3.0 mg/L 6-BA and 0.5 mg/L NAA produced more curcumin and other curcuminoids; however, the amount was less than in microrhizomes grown on 3% sucrose alone. We determined that a 5% sucrose medium supplemented with 3.0 mg/L of 6-BA and 0.5 mg/L of NAA enhanced the levels of curcumin and curcuminoids and that exposure to red light further increased production. PMID:25722985

  19. Western grebe, Aechmophorus occidentalis, wintering biology and contaminant accumulation in Commencement Bay, Puget Sound, Washington

    USGS Publications Warehouse

    Henny, C.J.; Blus, L.J.; Grove, R.A.

    1990-01-01

    Western Grebes wintering at the head of Commencement Bay (bordering the waterways) accumulated significant amounts of mercury, arsenic, DDE, PCBs, chlordanes, and perhaps cadmium and HCB between 17 October 1985 and 6 February 1986. No change in selenium or lead was detected, but copper declined significantly. Western Grebes were likely to accumulate even higher levels of certain contaminants because they remained in Commencement Bay for an additional 3 months after the final collection. Remige moult and bursa length were used to separate five individuals believed to represent one age class, from the remainder of the October collection. These birds, perhaps nonbreeders spending one continuous year in Puget Sound, contained the 5 highest PCB, 5 of the 8 highest DDE, and 2 of the 3 highest mercury concentrations. No evidence was found in the literature to suggest the contaminant concentrations we reported would adversely impact the Western Grebe population. As expected, lipid content of carcasses increased significantly from October (15.5%) to February (28.8%).

  20. Methyl jasmonate, yeast extract and sucrose stimulate phenolic acids accumulation in Eryngium planum L. shoot cultures.

    PubMed

    Kikowska, Małgorzata; Kędziora, Izabela; Krawczyk, Aldona; Thiem, Barbara

    2015-01-01

    Eryngium planum L. has been reported as a medicinal plant used in traditional medicine in Europe. The tissue cultures may be an alternative source of the biomass rich in desired bioactive compounds. The purpose of this study was to investigate the influence of the biotechnological techniques on the selected phenolic acids accumulation in the agitated shoot cultures of E. planum. Qualitative and quantitative analyses of those compounds in 50% aqueous - methanolic extracts from the biomass were conducted by applying the HPLC method. Methyl jasmonate (MeJA), yeast extract (YE) and sucrose (Suc) stimulated accumulation of the phenolic acids: rosmarinic (RA), chlorogenic (CGA) and caffeic (CA) in in vitro shoot cultures. Cultivation of shoots in liquid MS media supplemented with 1.0 mg L(-1) 6-benzyladenine and 0.1 mg L(-1) indole-3-acetic acid in the presence of 100 µM MeJA for 48h was an optimum condition of elicitation and resulted in approximately 4.5-fold increased content of RA + CGA + CA in plant material compared to the control (19.795 mg g(-1) DW, 4.36 mg g(-1) DW, respectively). The results provide the first evidence that the selected phenolic acids can be synthesized in elicited shoot cultures of flat sea holly in higher amount than in untreated shoots. PMID:25856557

  1. Chemical Form Matters: Differential Accumulation of Mercury Following Inorganic and Organic Mercury Exposures in Zebrafish Larvae

    SciTech Connect

    Korbas, Malgorzata; MacDonald, Tracy C.; Pickering, Ingrid J.; George, Graham N.; Krone, Patrick H.

    2013-04-08

    Mercury, one of the most toxic elements, exists in various chemical forms each with different toxicities and health implications. Some methylated mercury forms, one of which exists in fish and other seafood products, pose a potential threat, especially during embryonic and early postnatal development. Despite global concerns, little is known about the mechanisms underlying transport and toxicity of different mercury species. To investigate the impact of different mercury chemical forms on vertebrate development, we have successfully combined the zebrafish, a well-established developmental biology model system, with synchrotron-based X-ray fluorescence imaging. Our work revealed substantial differences in tissue-specific accumulation patterns of mercury in zebrafish larvae exposed to four different mercury formulations in water. Methylmercury species not only resulted in overall higher mercury burdens but also targeted different cells and tissues than their inorganic counterparts, thus revealing a significant role of speciation in cellular and molecular targeting and mercury sequestration. For methylmercury species, the highest mercury concentrations were in the eye lens epithelial cells, independent of the formulation ligand (chloride versus L-cysteine). For inorganic mercury species, in absence of L-cysteine, the olfactory epithelium and kidney accumulated the greatest amounts of mercury. However, with L-cysteine present in the treatment solution, mercuric bis-L-cysteineate species dominated the treatment, significantly decreasing uptake. Our results clearly demonstrate that the common differentiation between organic and inorganic mercury is not sufficient to determine the toxicity of various mercury species.

  2. The Use of Horizontal Wells in Gas Production from Hydrate Accumulations

    SciTech Connect

    Reagan, Matthew; Moridis, George J.; Reagan, Matthew T.; Zhang, Keni

    2008-04-15

    The amounts of hydrocarbon gases trapped in natural hydrate accumulations are enormous, leading to a recent interest in the evaluation of their potential as an energy source. Earlier studies have demonstrated that large volumes of gas can be readily produced at high rates for long times from gas hydrate accumulations by means of depressurization-induced dissociation, using conventional technology and vertical wells. The results of this numerical study indicate that the use of horizontal wells does not confer any practical advantages to gas production from Class 1 deposits. This is because of the large disparity in permeabilities between the hydrate layer (HL) and the underlying free gas zone, leading to a hydrate dissociation that proceeds in a horizontally dominant direction and is uniform along the length of the reservoir. When horizontal wells are placed near the base of the HL in Class 2 deposits, the delay in the evolution of a significant gas production rate outweighs their advantages, which include higher rates and the prevention of flow obstruction problems that often hamper the performance of vertical wells. Conversely, placement of a horizontal well near to top of the HL can lead to dramatic increases in gas production from Class 2 and Class 3 deposits over the corresponding production from vertical wells.

  3. Accumulation of plutonium in mammalian wildlife tissues following dispersal by accidental-release tests.

    PubMed

    Johansen, M P; Child, D P; Caffrey, E A; Davis, E; Harrison, J J; Hotchkis, M A C; Payne, T E; Ikeda-Ohno, A; Thiruvoth, S; Twining, J R; Beresford, N A

    2016-01-01

    We examined the distribution of plutonium (Pu) in the tissues of mammalian wildlife inhabiting the relatively undisturbed, semi-arid former Taranaki weapons test site, Maralinga, Australia. The accumulation of absorbed Pu was highest in the skeleton (83% ± 6%), followed by muscle (10% ± 9%), liver (6% ± 6%), kidneys (0.6% ± 0.4%), and blood (0.2%). Pu activity concentrations in lung tissues were elevated relative to the body average. Foetal transfer was higher in the wildlife data than in previous laboratory studies. The amount of Pu in the gastrointestinal tract was highly elevated relative to that absorbed within the body, potentially increasing transfer of Pu to wildlife and human consumers that may ingest gastrointestinal tract organs. The Pu distribution in the Maralinga mammalian wildlife generally aligns with previous studies related to environmental exposure (e.g. Pu in humans from worldwide fallout), but contrasts with the partitioning models that have traditionally been used for human worker-protection purposes (approximately equal deposition in bone and liver) which appear to under-predict the skeletal accumulation in environmental exposure conditions. PMID:25910926

  4. Embryonic sex steroid hormones accumulate in the eggshell of loggerhead sea turtle (Caretta caretta).

    PubMed

    Kobayashi, Shohei; Saito, Yoshimichi; Osawa, Akihisa; Katsumata, Etsuko; Karaki, Isuke; Nagaoka, Kentaro; Taya, Kazuyoshi; Watanabe, Gen

    2015-12-01

    Steroids hormones such as estradiol-17β (E2) and testosterone (T) are involved in gonadal differentiation of oviparous animals with temperature-dependent sex determination (TSD), and are greatly distributed. This hypothesizes that these embryonic steroid hormones probably accumulate in the eggshell throughout blood or/and chorioallantoic fluid in sea turtle species with TSD, producing females at higher temperature. To demonstrate this hypothesis, concentrations of E2 and T in the blood plasma from the hatchling loggerhead sea turtle (Caretta caretta) and in their eggshells were measured by radioimmunoassay. In the present study we propose that both concentrations of E2 and T in the blood plasma are correlated with amounts of these sex steroids in the eggshell. Moreover, contents of E2 in the eggshell showed a significant positive correlation with mean incubation temperatures during a thermosensitive period in the experimental nests, whereas T contents in the eggshell did not. Taken together, these findings indicated that embryonic E2 and T that accumulated in the eggshell can be extracted and measured. Furthermore, the present study suggested that contents of E2 in the eggshell may differ between male and female, and monitoring of these steroids is a useful method to identify the sex of loggerhead sea turtle hatchling. PMID:26050561

  5. Chromoplast biogenesis and carotenoid accumulation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Chromoplasts are special organelles that possess superior ability to synthesize and store massive amounts of carotenoids. They are responsible for the distinctive colors found in fruits, flowers, and roots. Chromoplasts exhibit various morphologies and are derived from either pre-existing chloroplas...

  6. High-stage analog accumulator for TDI CMOS image sensors

    NASA Astrophysics Data System (ADS)

    Jianxin, Li; Fujun, Huang; Yong, Zong; Jing, Gao

    2016-02-01

    The impact of the parasitic phenomenon on the performance of the analog accumulator in TDI CMOS image sensor is analyzed and resolved. A 128-stage optimized accumulator based on 0.18-μm one-poly four-metal 3.3 V CMOS technology is designed and simulated. A charge injection effect from the top plate sampling is employed to compensate the un-eliminated parasitics based on the accumulator with a decoupling switch, and then a calibration circuit is designed to restrain the mismatch and Process, Voltage and Temperature (PVT) variations. The post layout simulation indicates that the improved SNR of the accumulator upgrades from 17.835 to 21.067 dB, while an ideal value is 21.072 dB. In addition, the linearity of the accumulator is 99.62%. The simulation results of two extreme cases and Monte Carlo show that the mismatch and PVT variations are restrained by the calibration circuit. Furthermore, it is promising to design a higher stage accumulator based on the proposed structure. Project supported by the National Natural Science Foundation of China (Nos. 61404090, 61434004).

  7. Metabolic Adaptation in Transplastomic Plants Massively Accumulating Recombinant Proteins

    PubMed Central

    Bally, Julia; Job, Claudette; Belghazi, Maya; Job, Dominique

    2011-01-01

    Background Recombinant chloroplasts are endowed with an astonishing capacity to accumulate foreign proteins. However, knowledge about the impact on resident proteins of such high levels of recombinant protein accumulation is lacking. Methodology/Principal Findings Here we used proteomics to characterize tobacco (Nicotiana tabacum) plastid transformants massively accumulating a p-hydroxyphenyl pyruvate dioxygenase (HPPD) or a green fluorescent protein (GFP). While under the conditions used no obvious modifications in plant phenotype could be observed, these proteins accumulated to even higher levels than ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco), the most abundant protein on the planet. This accumulation occurred at the expense of a limited number of leaf proteins including Rubisco. In particular, enzymes involved in CO2 metabolism such as nuclear-encoded plastidial Calvin cycle enzymes and mitochondrial glycine decarboxylase were found to adjust their accumulation level to these novel physiological conditions. Conclusions/Significance The results document how protein synthetic capacity is limited in plant cells. They may provide new avenues to evaluate possible bottlenecks in recombinant protein technology and to maintain plant fitness in future studies aiming at producing recombinant proteins of interest through chloroplast transformation. PMID:21966485

  8. 26 CFR 1.662(a)-1 - Inclusion of amounts in gross income of beneficiaries of estates and complex trusts; general.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 8 2010-04-01 2010-04-01 false Inclusion of amounts in gross income of beneficiaries of estates and complex trusts; general. 1.662(a)-1 Section 1.662(a)-1 Internal Revenue INTERNAL... Trusts Which May Accumulate Income Or Which Distribute Corpus § 1.662(a)-1 Inclusion of amounts in...

  9. 13 CFR 108.2020 - Amount of Operational Assistance grant.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    .... (c) Pro rata reductions. In the event that the total amount of funds available to SBA for purposes of... in the amounts described in paragraphs (a) and (b) of this section, SBA will make pro rata...

  10. 20 CFR 341.5 - Amount of reimbursement.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... STATUTORY LIEN WHERE SICKNESS BENEFITS PAID § 341.5 Amount of reimbursement. (a) The Board shall receive as reimbursement the lesser of: (1) The amount of sickness benefits paid to the employee for the infirmity...

  11. 20 CFR 341.5 - Amount of reimbursement.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... STATUTORY LIEN WHERE SICKNESS BENEFITS PAID § 341.5 Amount of reimbursement. (a) The Board shall receive as reimbursement the lesser of: (1) The amount of sickness benefits paid to the employee for the infirmity...

  12. 20 CFR 341.5 - Amount of reimbursement.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... STATUTORY LIEN WHERE SICKNESS BENEFITS PAID § 341.5 Amount of reimbursement. (a) The Board shall receive as reimbursement the lesser of: (1) The amount of sickness benefits paid to the employee for the infirmity...

  13. 20 CFR 341.5 - Amount of reimbursement.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... STATUTORY LIEN WHERE SICKNESS BENEFITS PAID § 341.5 Amount of reimbursement. (a) The Board shall receive as reimbursement the lesser of: (1) The amount of sickness benefits paid to the employee for the infirmity...

  14. 20 CFR 341.5 - Amount of reimbursement.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... STATUTORY LIEN WHERE SICKNESS BENEFITS PAID § 341.5 Amount of reimbursement. (a) The Board shall receive as reimbursement the lesser of: (1) The amount of sickness benefits paid to the employee for the infirmity...

  15. Socially-Imposed Costs of Higher Education.

    ERIC Educational Resources Information Center

    Bowen, Howard R.

    The growth of higher educational expenditures attributable to new social demands is considered. The main objective is to identify the sources of these costs, to estimate their amount, and to judge their impact on higher education. Colleges and universities, like many other types of organizations, are subject to social pressures and governmental…

  16. Marketing Higher Education in the Eighties.

    ERIC Educational Resources Information Center

    Andrew, Loyd D.; And Others

    The responsiveness of higher education to changing market conditions was assessed in five studies. Two of the studies (information gap studies) were designed to determine the amount of information and interest in higher education held by male and female adults. Another study (community college survey) determined the extent to which community…

  17. Are Public Subsidies to Higher Education Regressive?

    ERIC Educational Resources Information Center

    Johnson, William R.

    2006-01-01

    This article estimates the dollar amount of public higher education subsidies received by U.S. youth and examines the distribution of subsidies and the taxes that finance them across parental and student income levels. Although youths from high-income families obtain more benefit from higher education subsidies, high-income households pay…

  18. 32 CFR 229.16 - Civil penalty amounts.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 32 National Defense 2 2011-07-01 2011-07-01 false Civil penalty amounts. 229.16 Section 229.16 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS PROTECTION OF ARCHAEOLOGICAL RESOURCES: UNIFORM REGULATIONS § 229.16 Civil penalty amounts. (a) Maximum amount of penalty. (1) Where...

  19. 18 CFR 1312.16 - Civil penalty amounts.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Civil penalty amounts. 1312.16 Section 1312.16 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROTECTION OF ARCHAEOLOGICAL RESOURCES: UNIFORM REGULATIONS § 1312.16 Civil penalty amounts. (a) Maximum amount of penalty. (1) Where the person...

  20. 36 CFR 296.16 - Civil penalty amounts.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 2 2013-07-01 2013-07-01 false Civil penalty amounts. 296.16 Section 296.16 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE PROTECTION OF ARCHAEOLOGICAL RESOURCES: UNIFORM REGULATIONS § 296.16 Civil penalty amounts. (a) Maximum amount of penalty. (1) Where the person...

  1. 32 CFR 229.16 - Civil penalty amounts.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 32 National Defense 2 2013-07-01 2013-07-01 false Civil penalty amounts. 229.16 Section 229.16 National Defense Department of Defense (Continued) OFFICE OF THE SECRETARY OF DEFENSE (CONTINUED) MISCELLANEOUS PROTECTION OF ARCHAEOLOGICAL RESOURCES: UNIFORM REGULATIONS § 229.16 Civil penalty amounts. (a) Maximum amount of penalty. (1) Where...

  2. 43 CFR 7.16 - Civil penalty amounts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 1 2011-10-01 2011-10-01 false Civil penalty amounts. 7.16 Section 7.16 Public Lands: Interior Office of the Secretary of the Interior PROTECTION OF ARCHAEOLOGICAL RESOURCES Uniform Regulations § 7.16 Civil penalty amounts. (a) Maximum amount of penalty. (1) Where the person being assessed a civil penalty has...

  3. 77 FR 61423 - Notice of Adjustment of Disaster Grant Amounts

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-09

    ... SECURITY Federal Emergency Management Agency Notice of Adjustment of Disaster Grant Amounts AGENCY: Federal... amount for Small Project Grants to State and local governments and private nonprofit facilities for...), 42 U.S.C. 5121-5207, prescribes that FEMA must annually adjust the maximum grant amount made...

  4. 42 CFR 57.2207 - Amount of scholarship grant.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Amount of scholarship grant. 57.2207 Section 57... CONSTRUCTION OF TEACHING FACILITIES, EDUCATIONAL IMPROVEMENTS, SCHOLARSHIPS AND STUDENT LOANS Physician Shortage Area Scholarship Grants § 57.2207 Amount of scholarship grant. (a) The amount of the...

  5. 42 CFR 57.2207 - Amount of scholarship grant.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 1 2013-10-01 2013-10-01 false Amount of scholarship grant. 57.2207 Section 57... CONSTRUCTION OF TEACHING FACILITIES, EDUCATIONAL IMPROVEMENTS, SCHOLARSHIPS AND STUDENT LOANS Physician Shortage Area Scholarship Grants § 57.2207 Amount of scholarship grant. (a) The amount of the...

  6. 42 CFR 57.2207 - Amount of scholarship grant.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false Amount of scholarship grant. 57.2207 Section 57... CONSTRUCTION OF TEACHING FACILITIES, EDUCATIONAL IMPROVEMENTS, SCHOLARSHIPS AND STUDENT LOANS Physician Shortage Area Scholarship Grants § 57.2207 Amount of scholarship grant. (a) The amount of the...

  7. 42 CFR 57.2207 - Amount of scholarship grant.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Amount of scholarship grant. 57.2207 Section 57... CONSTRUCTION OF TEACHING FACILITIES, EDUCATIONAL IMPROVEMENTS, SCHOLARSHIPS AND STUDENT LOANS Physician Shortage Area Scholarship Grants § 57.2207 Amount of scholarship grant. (a) The amount of the...

  8. 42 CFR 57.2207 - Amount of scholarship grant.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false Amount of scholarship grant. 57.2207 Section 57... CONSTRUCTION OF TEACHING FACILITIES, EDUCATIONAL IMPROVEMENTS, SCHOLARSHIPS AND STUDENT LOANS Physician Shortage Area Scholarship Grants § 57.2207 Amount of scholarship grant. (a) The amount of the...

  9. 76 FR 63933 - Notice of Adjustment of Disaster Grant Amounts

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-14

    ... SECURITY Notice of Adjustment of Disaster Grant Amounts AGENCY: Federal Emergency Management Agency, DHS. ACTION: Notice. SUMMARY: FEMA gives notice of an increase of the maximum amount for Small Project Grants.... 5121-5207, prescribes that FEMA must annually adjust the maximum grant amount made under section...

  10. 46 CFR 249.3 - Amounts of insurance.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... OPERATORS APPROVAL OF UNDERWRITERS FOR MARINE HULL INSURANCE § 249.3 Amounts of insurance. MARAD will inform... 46 Shipping 8 2010-10-01 2010-10-01 false Amounts of insurance. 249.3 Section 249.3 Shipping... initial placement and at least annually thereafter, of the minimum amount of insurance required to...

  11. 5 CFR 870.904 - Amount of insurance.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Amount of insurance. 870.904 Section 870.904 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE REGULATIONS (CONTINUED) FEDERAL EMPLOYEES' GROUP LIFE INSURANCE PROGRAM Assignments of Life Insurance § 870.904 Amount of insurance. The amount of insurance is...

  12. 5 CFR 870.702 - Amount of Basic insurance.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Amount of Basic insurance. 870.702 Section 870.702 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE REGULATIONS (CONTINUED) FEDERAL EMPLOYEES' GROUP LIFE INSURANCE PROGRAM Annuitants and Compensationers § 870.702 Amount of Basic insurance. (a) The amount...

  13. 5 CFR 870.202 - Basic insurance amount (BIA).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Basic insurance amount (BIA). 870.202 Section 870.202 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE REGULATIONS (CONTINUED) FEDERAL EMPLOYEES' GROUP LIFE INSURANCE PROGRAM Types and Amount of Insurance § 870.202 Basic insurance amount (BIA). (a)(1)...

  14. 5 CFR 870.205 - Amount of Optional insurance.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Amount of Optional insurance. 870.205 Section 870.205 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT (CONTINUED) CIVIL SERVICE REGULATIONS (CONTINUED) FEDERAL EMPLOYEES' GROUP LIFE INSURANCE PROGRAM Types and Amount of Insurance § 870.205 Amount of Optional insurance. (a) Option...

  15. 24 CFR 761.13 - Amount of funding.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 4 2012-04-01 2012-04-01 false Amount of funding. 761.13 Section... PUBLIC AND INDIAN HOUSING PROGRAMS) DRUG ELIMINATION PROGRAMS Grant Funding § 761.13 Amount of funding. (a) PHDEP formula funding—(1) Funding share formula—(i) Per unit amount. Subject to the...

  16. 24 CFR 761.13 - Amount of funding.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 4 2013-04-01 2013-04-01 false Amount of funding. 761.13 Section... PUBLIC AND INDIAN HOUSING PROGRAMS) DRUG ELIMINATION PROGRAMS Grant Funding § 761.13 Amount of funding. (a) PHDEP formula funding—(1) Funding share formula—(i) Per unit amount. Subject to the...

  17. 7 CFR 3565.457 - Determination of claim amount.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 15 2010-01-01 2010-01-01 false Determination of claim amount. 3565.457 Section 3565....457 Determination of claim amount. In all liquidation cases, final settlement will be made with the...) The estimated loss payment shall be applied as of the date of such payment. The total amount of...

  18. 42 CFR 493.649 - Methodology for determining fee amount.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 5 2011-10-01 2011-10-01 false Methodology for determining fee amount. 493.649 Section 493.649 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN... Methodology for determining fee amount. (a) General rule. The amount of the fee in each schedule...

  19. 24 CFR 92.60 - Allocation amounts for insular areas.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... this section, or for any other reason, HUD may increase the allocation amount for one or more of the... requirements. Funds that become available but which are not used to increase the allocation amount for one or... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Allocation amounts for...

  20. 24 CFR 92.60 - Allocation amounts for insular areas.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... this section, or for any other reason, HUD may increase the allocation amount for one or more of the... requirements. Funds that become available but which are not used to increase the allocation amount for one or... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Allocation amounts for...

  1. 20 CFR 229.66 - Changes in reduction amount.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Under a Federal, State, or Local Law or Plan § 229.66 Changes in reduction amount. (a) Change in DIB O/M. The amount of the worker's compensation or public disability benefit reduction does not change when..., the reduction amount does change if there is a change in the family members included in the DIB...

  2. 20 CFR 229.66 - Changes in reduction amount.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Under a Federal, State, or Local Law or Plan § 229.66 Changes in reduction amount. (a) Change in DIB O/M. The amount of the worker's compensation or public disability benefit reduction does not change when..., the reduction amount does change if there is a change in the family members included in the DIB...

  3. 20 CFR 229.66 - Changes in reduction amount.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Under a Federal, State, or Local Law or Plan § 229.66 Changes in reduction amount. (a) Change in DIB O/M... decreases, the change in the reduction amount is effective with the month of the decrease, no matter when... 20 Employees' Benefits 1 2014-04-01 2012-04-01 true Changes in reduction amount. 229.66...

  4. 20 CFR 416.412 - Amount of benefits; eligible couple.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Amount of benefits; eligible couple. 416.412... AGED, BLIND, AND DISABLED Amount of Benefits § 416.412 Amount of benefits; eligible couple. The benefit under this part for an eligible couple (including couples where one or both members of the couple...

  5. 18 CFR 1312.16 - Civil penalty amounts.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 2 2013-04-01 2012-04-01 true Civil penalty amounts. 1312.16 Section 1312.16 Conservation of Power and Water Resources TENNESSEE VALLEY AUTHORITY PROTECTION OF ARCHAEOLOGICAL RESOURCES: UNIFORM REGULATIONS § 1312.16 Civil penalty amounts. (a) Maximum amount of penalty. (1) Where the person...

  6. 41 CFR 105-56.019 - Offset amount.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Administration 56-SALARY OFFSET FOR INDEBTEDNESS OF FEDERAL EMPLOYEES TO THE UNITED STATES Centralized Salary Offset (CSO) Procedures-GSA as Creditor Agency § 105-56.019 Offset amount. (a) The minimum dollar amount referred for offset under this subpart is $100. (b) The amount offset from a salary payment under...

  7. 41 CFR 105-56.029 - Offset amount.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Administration 56-SALARY OFFSET FOR INDEBTEDNESS OF FEDERAL EMPLOYEES TO THE UNITED STATES Centralized Salary Offset (CSO) Procedures-GSA as Paying Agency § 105-56.029 Offset amount. (a) The minimum dollar amount of salary offset under this subpart is $100. (b) The amount offset from a salary payment under this...

  8. 42 CFR 423.886 - Retiree drug subsidy amounts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 3 2011-10-01 2011-10-01 false Retiree drug subsidy amounts. 423.886 Section 423... (CONTINUED) MEDICARE PROGRAM VOLUNTARY MEDICARE PRESCRIPTION DRUG BENEFIT Payments to Sponsors of Retiree Prescription Drug Plans § 423.886 Retiree drug subsidy amounts. (a) Amount of subsidy payment. (1) For...

  9. 42 CFR 423.886 - Retiree drug subsidy amounts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 3 2010-10-01 2010-10-01 false Retiree drug subsidy amounts. 423.886 Section 423... (CONTINUED) MEDICARE PROGRAM VOLUNTARY MEDICARE PRESCRIPTION DRUG BENEFIT Payments to Sponsors of Retiree Prescription Drug Plans § 423.886 Retiree drug subsidy amounts. (a) Amount of subsidy payment. (1) For...

  10. 20 CFR 625.6 - Weekly amount; jurisdictions; reductions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... DISASTER UNEMPLOYMENT ASSISTANCE § 625.6 Weekly amount; jurisdictions; reductions. (a) In all States... worker or unemployed self-employed individual for a week of total unemployment shall be the weekly amount... provisions of the applicable State law for a week of total unemployment. In no event shall such amount be...

  11. 20 CFR 625.6 - Weekly amount; jurisdictions; reductions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... DISASTER UNEMPLOYMENT ASSISTANCE § 625.6 Weekly amount; jurisdictions; reductions. (a) In all States... worker or unemployed self-employed individual for a week of total unemployment shall be the weekly amount... provisions of the applicable State law for a week of total unemployment. In no event shall such amount be...

  12. 9 CFR 89.1 - Amount of feed.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... TWENTY-EIGHT HOUR LAW § 89.1 Amount of feed. (a) Under normal conditions, the amount of feed designated... at the intervals required by the Twenty-Eight Hour Law: Species and quantity of livestock At first... car, the amount of shelled corn 2 indicated): Lots of not more than 18,000 lbs 2 bushels 2...

  13. 9 CFR 89.1 - Amount of feed.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... TWENTY-EIGHT HOUR LAW § 89.1 Amount of feed. (a) Under normal conditions, the amount of feed designated... at the intervals required by the Twenty-Eight Hour Law: Species and quantity of livestock At first... car, the amount of shelled corn 2 indicated): Lots of not more than 18,000 lbs 2 bushels 2...

  14. 20 CFR 229.66 - Changes in reduction amount.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Under a Federal, State, or Local Law or Plan § 229.66 Changes in reduction amount. (a) Change in DIB O/M. The amount of the worker's compensation or public disability benefit reduction does not change when..., the reduction amount does change if there is a change in the family members included in the DIB...

  15. 20 CFR 229.66 - Changes in reduction amount.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Under a Federal, State, or Local Law or Plan § 229.66 Changes in reduction amount. (a) Change in DIB O/M. The amount of the worker's compensation or public disability benefit reduction does not change when..., the reduction amount does change if there is a change in the family members included in the DIB...

  16. 10 CFR 205.286 - Limitations on amount of refunds.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 3 2013-01-01 2013-01-01 false Limitations on amount of refunds. 205.286 Section 205.286 Energy DEPARTMENT OF ENERGY OIL ADMINISTRATIVE PROCEDURES AND SANCTIONS Special Procedures for Distribution of Refunds § 205.286 Limitations on amount of refunds. (a) The aggregate amount of all...

  17. 10 CFR 205.286 - Limitations on amount of refunds.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 3 2014-01-01 2014-01-01 false Limitations on amount of refunds. 205.286 Section 205.286 Energy DEPARTMENT OF ENERGY OIL ADMINISTRATIVE PROCEDURES AND SANCTIONS Special Procedures for Distribution of Refunds § 205.286 Limitations on amount of refunds. (a) The aggregate amount of all...

  18. 10 CFR 205.286 - Limitations on amount of refunds.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 3 2012-01-01 2012-01-01 false Limitations on amount of refunds. 205.286 Section 205.286 Energy DEPARTMENT OF ENERGY OIL ADMINISTRATIVE PROCEDURES AND SANCTIONS Special Procedures for Distribution of Refunds § 205.286 Limitations on amount of refunds. (a) The aggregate amount of all...

  19. 26 CFR 301.6401-1 - Amounts treated as overpayments.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 18 2014-04-01 2014-04-01 false Amounts treated as overpayments. 301.6401-1... General § 301.6401-1 Amounts treated as overpayments. (a) The term “overpayment” includes: (1) Any payment... limitation applicable thereto. (2) Any amount allowable for a taxable year as credits under sections...

  20. 26 CFR 301.6401-1 - Amounts treated as overpayments.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 18 2010-04-01 2010-04-01 false Amounts treated as overpayments. 301.6401-1... General § 301.6401-1 Amounts treated as overpayments. (a) The term “overpayment” includes: (1) Any payment... limitation applicable thereto. (2) Any amount allowable for a taxable year as credits under sections...

  1. 26 CFR 301.6401-1 - Amounts treated as overpayments.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 18 2012-04-01 2012-04-01 false Amounts treated as overpayments. 301.6401-1... General § 301.6401-1 Amounts treated as overpayments. (a) The term “overpayment” includes: (1) Any payment... limitation applicable thereto. (2) Any amount allowable for a taxable year as credits under sections...

  2. 26 CFR 301.6401-1 - Amounts treated as overpayments.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 18 2011-04-01 2011-04-01 false Amounts treated as overpayments. 301.6401-1... General § 301.6401-1 Amounts treated as overpayments. (a) The term “overpayment” includes: (1) Any payment... limitation applicable thereto. (2) Any amount allowable for a taxable year as credits under sections...

  3. 26 CFR 301.6401-1 - Amounts treated as overpayments.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 18 2013-04-01 2013-04-01 false Amounts treated as overpayments. 301.6401-1... General § 301.6401-1 Amounts treated as overpayments. (a) The term “overpayment” includes: (1) Any payment... limitation applicable thereto. (2) Any amount allowable for a taxable year as credits under sections...

  4. 12 CFR 1806.202 - Estimated award amounts.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Estimated award amounts. 1806.202 Section 1806... BANK ENTERPRISE AWARD PROGRAM Awards § 1806.202 Estimated award amounts. (a) General. An Applicant shall calculate and submit to the Fund an estimated award amount as part of the Bank Enterprise...

  5. 20 CFR 625.6 - Weekly amount; jurisdictions; reductions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... DISASTER UNEMPLOYMENT ASSISTANCE § 625.6 Weekly amount; jurisdictions; reductions. (a) In all States... worker or unemployed self-employed individual for a week of total unemployment shall be the weekly amount... provisions of the applicable State law for a week of total unemployment. In no event shall such amount be...

  6. 20 CFR 416.410 - Amount of benefits; eligible individual.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false Amount of benefits; eligible individual. 416.410 Section 416.410 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.410 Amount of benefits; eligible...

  7. 20 CFR 416.413 - Amount of benefits; qualified individual.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false Amount of benefits; qualified individual. 416.413 Section 416.413 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.413 Amount of benefits;...

  8. 20 CFR 416.412 - Amount of benefits; eligible couple.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false Amount of benefits; eligible couple. 416.412 Section 416.412 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.412 Amount of benefits; eligible couple. The...

  9. 42 CFR 423.886 - Retiree drug subsidy amounts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 3 2014-10-01 2014-10-01 false Retiree drug subsidy amounts. 423.886 Section 423... (CONTINUED) MEDICARE PROGRAM (CONTINUED) VOLUNTARY MEDICARE PRESCRIPTION DRUG BENEFIT Payments to Sponsors of Retiree Prescription Drug Plans § 423.886 Retiree drug subsidy amounts. (a) Amount of subsidy payment....

  10. 42 CFR 423.886 - Retiree drug subsidy amounts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 3 2012-10-01 2012-10-01 false Retiree drug subsidy amounts. 423.886 Section 423... (CONTINUED) MEDICARE PROGRAM (CONTINUED) VOLUNTARY MEDICARE PRESCRIPTION DRUG BENEFIT Payments to Sponsors of Retiree Prescription Drug Plans § 423.886 Retiree drug subsidy amounts. (a) Amount of subsidy payment....

  11. 42 CFR 423.886 - Retiree drug subsidy amounts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 3 2013-10-01 2013-10-01 false Retiree drug subsidy amounts. 423.886 Section 423... (CONTINUED) MEDICARE PROGRAM (CONTINUED) VOLUNTARY MEDICARE PRESCRIPTION DRUG BENEFIT Payments to Sponsors of Retiree Prescription Drug Plans § 423.886 Retiree drug subsidy amounts. (a) Amount of subsidy payment....

  12. 22 CFR 1104.15 - Civil penalty amounts.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Civil penalty amounts. 1104.15 Section 1104.15... SECTION PROTECTION OF ARCHAEOLOGICAL RESOURCES § 1104.15 Civil penalty amounts. (a) Maximum amount of penalty. (1) Where the person being assessed a civil penalty has not committed any previous violation...

  13. 20 CFR 416.413 - Amount of benefits; qualified individual.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false Amount of benefits; qualified individual. 416.413 Section 416.413 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.413 Amount of benefits;...

  14. 20 CFR 416.412 - Amount of benefits; eligible couple.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 2 2013-04-01 2013-04-01 false Amount of benefits; eligible couple. 416.412 Section 416.412 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.412 Amount of benefits; eligible couple. The...

  15. 20 CFR 416.413 - Amount of benefits; qualified individual.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Amount of benefits; qualified individual. 416.413 Section 416.413 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.413 Amount of benefits;...

  16. 20 CFR 416.412 - Amount of benefits; eligible couple.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false Amount of benefits; eligible couple. 416.412 Section 416.412 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.412 Amount of benefits; eligible couple. The...

  17. 20 CFR 416.412 - Amount of benefits; eligible couple.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Amount of benefits; eligible couple. 416.412 Section 416.412 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.412 Amount of benefits; eligible couple. The...

  18. 20 CFR 416.410 - Amount of benefits; eligible individual.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 2 2013-04-01 2013-04-01 false Amount of benefits; eligible individual. 416.410 Section 416.410 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.410 Amount of benefits; eligible...

  19. 20 CFR 416.413 - Amount of benefits; qualified individual.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 2 2013-04-01 2013-04-01 false Amount of benefits; qualified individual. 416.413 Section 416.413 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.413 Amount of benefits;...

  20. 20 CFR 416.410 - Amount of benefits; eligible individual.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false Amount of benefits; eligible individual. 416.410 Section 416.410 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.410 Amount of benefits; eligible...

  1. 20 CFR 416.410 - Amount of benefits; eligible individual.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Amount of benefits; eligible individual. 416.410 Section 416.410 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Amount of Benefits § 416.410 Amount of benefits; eligible...

  2. Modeling the Effect of Reward Amount on Probability Discounting

    ERIC Educational Resources Information Center

    Myerson, Joel; Green, Leonard; Morris, Joshua

    2011-01-01

    The present study with college students examined the effect of amount on the discounting of probabilistic monetary rewards. A hyperboloid function accurately described the discounting of hypothetical rewards ranging in amount from $20 to $10,000,000. The degree of discounting increased continuously with amount of probabilistic reward. This effect…

  3. [Man-made radionuclides and their accumulation by plants of different taxonomic groups from the soils of the eastern ural radioactive trace].

    PubMed

    Molchanova, I V; Mikhaĭlovskaia, L N; Pozolotina, V N; Antonova, E V

    2014-01-01

    Investigations of the spatial and vertical distribution of 90Sr, 137Cs and 239, 240Pu in the soils of the Eastern Ural Radioactive Trace (EURT) and accumulation of these radionuclides by plants were carried out in 2003-2011. Investigations showed that the spatial distribution of the radionuclides with increasing distances from the epicenter of the accident is satisfactorily approximated by the exponential function. During the post-accidental period the essential amount of radionuclides is located in the 15-20 cm root layer of the soil. Uptake by plants of 90Sr is determined, first of all, by the level of the soil contamination. For 137Cs, reliable differences in its accumulation ability between representatives of the higher plants and lower ones are remained in the whole gradient of contamination. PMID:25764849

  4. Betaine Accumulation and Betaine-Aldehyde Dehydrogenase in Spinach Leaves 1

    PubMed Central

    Pan, Shu-Mei; Moreau, Robert A.; Yu, Charles; Huang, Anthony H. C.

    1981-01-01

    Spinach leaf discs accumulated betaine when exposed to a mannitol solution of −20 bars. The accumulation was 12 micromoles per gram original fresh weight in a 24-hour period. Betaine-aldehyde dehydrogenase (EC 1.2.1.8) was assayed in various subcellular fractions prepared from spinach leaves, and it was found only in the soluble fraction. This cytosolic enzyme was purified 175-fold, and its properties were studied. The enzyme was relatively specific for betaine aldehyde as the substrate with an apparent Km value of 2.08 × 10−4 molar. It also exerted activity on other aldehyde analogs tested, but with lower Vmax and higher Km values. The enzyme was relatively specific for nicotinamide adenine dinucleotide as the coenzyme, having an apparent Km value of 9.46 × 10−6 molar; lower activities were observed when nicotinamide adenine dinucleotide phosphate or 3-acetyl pyridine adenine dinucleotide were tested as electron acceptors. The activity was enhanced by dithiothreitol and inhibited by p-chloromercuribenzoate, and the inhibition by p-chloromercuribenzoate was partially reversed by the subsequent addition of dithiothreitol. The activity was inhibited by high concentrations of NaCl and, to a lesser extent, proline. The equilibrium of the enzymic reaction was strongly in favor of betaine formation. The in vitro activity of the enzyme under optimal assay conditions was high enough to account for the amount of betaine accumulated under water stress conditions. The enzyme activity was the same in unstressed leaves and in leaves that had been water stressed for 24 hours. PMID:16661818

  5. Synergy of fresh and accumulated organic matter to bacterial growth.

    PubMed

    Farjalla, Vinicius F; Marinho, Claudio C; Faria, Bias M; Amado, André M; Esteves, Francisco de A; Bozelli, Reinaldo L; Giroldo, Danilo

    2009-05-01

    The main goal of this research was to evaluate whether the mixture of fresh labile dissolved organic matter (DOM) and accumulated refractory DOM influences bacterial production, respiration, and growth efficiency (BGE) in aquatic ecosystems. Bacterial batch cultures were set up using DOM leached from aquatic macrophytes as the fresh DOM pool and DOM accumulated from a tropical humic lagoon. Two sets of experiments were performed and bacterial growth was followed in cultures composed of each carbon substrate (first experiment) and by carbon substrates combined (second experiment), with and without the addition of nitrogen and phosphorus. In both experiments, bacterial production, respiration, and BGE were always higher in cultures with N and P additions, indicating a consistent inorganic nutrient limitation. Bacterial production, respiration, and BGE were higher in cultures set up with leachate DOM than in cultures set up with humic DOM, indicating that the quality of the organic matter pool influenced the bacterial growth. Bacterial production and respiration were higher in the mixture of substrates (second experiment) than expected by bacterial production and respiration in single substrate cultures (first experiment). We suggest that the differences in the concentration of some compounds between DOM sources, the co-metabolism on carbon compound decomposition, and the higher diversity of molecules possibly support a greater bacterial diversity which might explain the higher bacterial growth observed. Finally, our results indicate that the mixture of fresh labile and accumulated refractory DOM that naturally occurs in aquatic ecosystems could accelerate the bacterial growth and bacterial DOM removal. PMID:18985269

  6. Racial Differences in Patterns of Wealth Accumulation

    ERIC Educational Resources Information Center

    Gittleman, Maury; Wolff, Edward N.

    2004-01-01

    The race differences in patterns of asset accumulations were examined using PSD data for 1984, 1989 and 1994. The results indicate that inheritances led to wealth accumulations among whites as compared to the African Americans.

  7. Neurodegeneration with brain iron accumulation (NBIA)

    MedlinePlus

    ... gov/ency/article/001225.htm Neurodegeneration with brain iron accumulation (NBIA) To use the sharing features on this page, please enable JavaScript. Neurodegeneration with brain iron accumulation (formerly known as Hallervorden-Spatz disease) is ...

  8. Cadmium accumulation in leaves of leafy vegetables.

    PubMed

    Baldantoni, Daniela; Morra, Luigi; Zaccardelli, Massimo; Alfani, Anna

    2016-01-01

    Leafy vegetables have a relatively high potential for Cd uptake and translocation, and are thus considered Cd accumulators. For this reason, leaves and roots of lettuce (Lactuca sativa L.) and endive (Cichorium endivia L.) plants, grown on different agricultural soils in Campania region (southern Italy), subjected to different fertilisation treatments (unfertilisation, compost amendment and mineral fertilisation), were analysed for Cd concentrations. Moreover, to clarify if the highest concentrations found are linked to older and inedible or to younger and edible leaves, external and internal endive leaves were separately analysed. All the leafy vegetables analysed showed on average 2-fold higher Cd concentrations in leaves than in roots. Leaf Cd concentrations in both lettuce and endive plants significantly differed among fertilisation treatments, with values highest in the plants grown on mineral fertilised soils. Apart from the soil fertilisation treatments, however, Cd leaf concentrations were often higher (up to 4-fold) than the threshold deduced by the EU 420/2011 Regulation, although the plants grew on unpolluted soils. Anyway, external leaves of endive plants showed significantly higher concentrations than internal leaves (in some cases the values were 3-fold higher), partly reassuring on the consumption of the younger leaves. Moreover, this study points out two major drawbacks in the Italian and European regulatory frameworks: (1) metal concentration (as total and/or available fraction) limits in agricultural soils are lacking; (2) metal concentration thresholds (currently existing only for Cd and Pb in crops) reported in the EU 420/2011 Regulation, expressed on the fresh weight basis rather than on the dry weight basis, appear not suitable. PMID:26004982

  9. Calcium: Some aspects of subcellular accumulation and distribution in milk

    SciTech Connect

    Shappell, N.W.

    1989-01-01

    Distribution and bioavailability of {sup 47}calcium in milk labeled by extrinsic and intrinsic methods was investigated. Milk from Sprague Dawley rats was labeled by both methods, and milk from a cow was labeled by the extrinsic method. Retention of {sup 47}Ca from milks administered to young male Sprague Dawley rats was determined through whole body counting for 6 days after administration of milk. Percent of {sup 47}Ca dose retained was 72% for extrinsically labeled cow milk, 62% for extrinsically labeled rat milk, and 55% for intrinsically labeled rat milk. Samples were fractionated by ultracentrifugation and by gel exclusion chromatography. {sup 47}Calcium distributions in rat milk labeled intrinsically or extrinsically were similar. The majority of {sup 47}Ca was found in a particulate, >30,000 molecular weight fraction. The amount of milk calcium retained by rats appeared to be related to the amount of noncasein micelle-associated calcium. When administered by intraperitoneal injection into rats, {sup 45}Ca specific activity of milk peaked in 60 to 90 minutes. In vitro {sup 45}Ca accumulation was compared in Golgi apparatus and endoplasmic reticulum from liver and mammary gland of lactating Dunkin Hartley guinea pigs. In the presence of ATP, highest accumulation per unit total fraction protein was found in Golgi apparatus (mammary gland 28% of available {sup 45}Ca, liver 11%) while 8% was accumulated by endoplasmic reticulum fractions.

  10. Accumulation and depuration profiles of PSP toxins in the short-necked clam Tapes japonica fed with the toxic dinoflagellate Alexandrium catenella.

    PubMed

    Samsur, Mohamad; Yamaguchi, Yasunaga; Sagara, Takefumi; Takatani, Tomohiro; Arakawa, Osamu; Noguchi, Tamao

    2006-09-01

    A toxic dinoflagellate responsible for paralytic shellfish poisoning (PSP), Alexandrium catenella (Ac) was fed to the short-necked clam Tapes japonica, and the accumulation and depuration profiles of PSP toxins were investigated by means of high-performance liquid chromatography with postcolumn fluorescence derivatization (HPLC-FLD). The short-necked clams ingested more than 99% of the Ac cells (4 x 10(7)cells) supplied once at the beginning of experiment, and accumulated a maximal amount of toxin (185 nmol/10 clams) after 12h. The rate of toxin accumulation at that time was 23%, which rapidly decreased thereafter. Composition of the PSP toxin accumulated in the clams obviously different from that of Ac even 0.5h after the cell supply, the proportion of C1+2 being much higher than in Ac, although the reason remains to be elucidated. In contrast, a higher ratio of gonyautoxin (GTX)1+4 than in Ac was detected in the toxin profiles of clam excrements. The variation in toxin composition derived presumably from the transformation of toxin analogues in clams was observed from 0.5h, such as reversal of the ratio of C1 to C2, and appearance of carbamate (saxitoxin (STX), neoSTX and GTX2, 3) and decarbamoyl (dc) derivatives (dcSTX and dcGTX2, 3), which were undetectable in Ac cells. The total amount of toxin distributed over Ac cells, clams and their excrements gradually declined, and only 1% of supplied toxin was detected at the end of experiment. PMID:16887162

  11. Micropatterned multienzyme devices with adjustable amounts of immobilized enzymes.

    PubMed

    Burchardt, Malte; Wittstock, Gunther

    2013-12-01

    Multienzyme microstructures of glucose oxidase (GOx) and horseradish peroxidase (HRP) were prepared by layer-by-layer deposition inside microfluidic networks on glass substrates in order to allow both site-specific deposition and control of the amount of immobilized enzymes. The obtained microstructures were characterized by scanning force microscopy for the topography of the deposited layers. The local enzyme activity was characterized by the substrate-generation/tip-collection mode and the enzyme-mediated feedback mode of the scanning electrochemical microscope (SECM). These measurements provided quantitative information about the immobilized enzyme activity as a basis for adjusting enzyme loading for multienzyme structures that realize logical operations based on enzymatic conversions. Information about local HRP activity can also be obtained by optical readout using an Amplex UltraRed fluorgenic substrate and reading with a confocal laser scanning microscope with a much higher repetition rate for image acquisition. Using these principles, a layout with HRP and GOx microstructures was realized that showed the functionality of an OR Boolean logic switch. PMID:24200032

  12. 46 CFR 58.30-25 - Accumulators.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Accumulators. 58.30-25 Section 58.30-25 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING MAIN AND AUXILIARY MACHINERY AND RELATED SYSTEMS Fluid Power and Control Systems § 58.30-25 Accumulators. (a) An accumulator is an unfired pressure vessel in which energy is...

  13. 42 CFR 419.41 - Calculation of national beneficiary copayment amounts and national Medicare program payment amounts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... amounts and national Medicare program payment amounts. 419.41 Section 419.41 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM... § 419.41 Calculation of national beneficiary copayment amounts and national Medicare program...

  14. 42 CFR 419.41 - Calculation of national beneficiary copayment amounts and national Medicare program payment amounts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... amounts and national Medicare program payment amounts. 419.41 Section 419.41 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM... § 419.41 Calculation of national beneficiary copayment amounts and national Medicare program...

  15. 42 CFR 419.41 - Calculation of national beneficiary copayment amounts and national Medicare program payment amounts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... amounts and national Medicare program payment amounts. 419.41 Section 419.41 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM... § 419.41 Calculation of national beneficiary copayment amounts and national Medicare program...

  16. Comparative Studies on Plastoquinones. III. Distribution of Plastoquinones in Higher Plants 1

    PubMed Central

    Barr, Rita; Crane, F. L.

    1967-01-01

    The distribution of plastoquinones A 45, B and C was studied in representatives from 34 different plant families beginning with liverworts and mosses to higher plants. All of these species, including many monocots and dicots, contained significant amounts of the 3 quinones. Two species of Aesculus contained plastoquinone A 20 in addition to plastoquinone A 45, B, and C. Many dicots, such as Aesculus, watermelon, tobacco and tomato accumulated increasing quantities of plastoquinones A and C1-C4 during the growing season. The concentrations of plastoquinones B and C5-C6 tended to remain at a constant low level during the season (<0.01 μmole per mg chlorophyll). Preliminary studies with bean plants (Vicia faba and Phaseolus sp.) indicate that the levels of quinones varied little under different growth conditions (day length and temp.) although Vicia faba tended to have higher PQ A values with increased temperature. PMID:16656647

  17. Chip integrated fuel cell accumulator

    NASA Astrophysics Data System (ADS)

    Frank, M.; Erdler, G.; Frerichs, H.-P.; Müller, C.; Reinecke, H.

    A unique new design of a chip integrated fuel cell accumulator is presented. The system combines an electrolyser and a self-breathing polymer electrolyte membrane (PEM) fuel cell with integrated palladium hydrogen storage on a silicon substrate. Outstanding advantages of this assembly are the fuel cell with integrated hydrogen storage, the possibility of refuelling it by electrolysis and the opportunity of simply refilling the electrolyte by adding water. By applying an electrical current, wiring the palladium hydrogen storage as cathode and the counter-electrode as anode, the electrolyser produces hydrogen at the palladium surface and oxygen at the electrolyser cell anode. The generated hydrogen is absorbed by the palladium electrode and the hydrogen storage is refilled consequently enabling the fuel cell to function.

  18. Over-accumulation of nuclear IGF-1 receptor in tumor cells requires elevated expression of the receptor and the SUMO-conjugating enzyme Ubc9

    SciTech Connect

    Deng, Hua; Lin, Yingbo; Badin, Margherita; Vasilcanu, Daiana; Stroemberg, Thomas; Jernberg-Wiklund, Helena; Sehat, Bita; Larsson, Olle

    2011-01-14

    Research highlights: {yields} SUMOylation mediates nuclear translocation of IGF-1R which activates transcription. {yields} Here we show that nuclear IGF-1R over-accumulates in tumor cells. {yields} This requires overexpression of the receptor that is a common feature in tumor cells. {yields} An increased expression of the SUMO ligase Ubc9 seems to be an involved mechanism too. -- Abstract: The insulin-like growth factor 1 receptor (IGF-1R) plays crucial roles in tumor cell growth and is overexpressed in many cancers. IGF-1R's trans-membrane kinase signaling pathways have been well characterized. Very recently, we showed that SUMOylation mediates nuclear translocation of the IGF-1R, and that nuclear IGF-1R (nIGF-1R) binds to enhancer regions and activates transcription. We identified three lysine residues in the {beta}-subunit of the receptor and that mutation of these blocks nuclear translocation and gene activation. Furthermore, accumulation of nIGF-1R was proven strongly dependent on the specific SUMO-conjugating enzyme Ubc9. Here we show that nIGF-1R originates solely from the cell membrane and that phosphorylation of the core tyrosine residues of the receptor kinase is crucial for nuclear accumulation. We also compared the levels of nIGF-1R, measured as nuclear/membrane ratios, in tumor and normal cells. We found that the breast cancer cell line MCF-7 has 13-fold higher amounts of nIGF-1R than breast epithelial cells (IME) which showed only a small amount of nIGF-1R. In comparison, the total expression of IGF-1R was only 3.7- higher in MCF-7. Comparison of several other tumor and normal cell lines showed similar tumor cell over-accumulation of nIGF-1R, exceeding the total receptor expression substantially. Ectopic overexpression (>10-fold) of the receptor increased nIGF-1R in IME cells but not to that high level as in wild type MCF-7. The levels of Ubc9 were higher in all tumor cell lines, compared to the normal cells, and this probably contributes to over-accumulation

  19. Corrosion analysis of accumulative roll bonded aluminum 6016

    NASA Astrophysics Data System (ADS)

    Searcy, Jacquelyn Alisha

    Accumulative Roll Bonding is a Severe Plastic Deformation Process that is used to strengthen a material and promote grain refinement. Accumulative Roll Bonded Aluminum 6016 samples were investigated to determine their corrosion properties. The tests performed consisted of standard techniques including Cyclic Polarization Potentials, Exfoliation Corrosion (EXCO), Electrochemical Impedance Spectroscopy (EIS), Light Microscopy, and Electron Microprobe Analysis. From these tests, it was determined that for Al 6016, the Ultra Fine Grained samples obtained by Accumulative Roll Bonding are in general more susceptible to corrosion than the coarse grained sample. The higher corrosion rate was caused by the additional cold work, which increased the number of grain boundaries and rolled-in debris. The advantage however was that the corrosion was parallel to the surface and rather than deep into the sample as with the as-received 6016.

  20. Accumulation of polycyclic aromatic hydrocarbons in acid sensitive lakes

    SciTech Connect

    Furlong, E.T.; Cessar, L.R.; Hites, R.A. )

    1987-11-01

    Polycyclic aromatic hydrocarbon concentrations and fluxes were measured in {sup 210}Pb dated sediment cores taken from nine lakes in four regions identified as susceptible to acidification. Calculated PAH accumulations were compared with historic S emissions, accumulation of sedimentary S, and anthropogenic metal accumulations to determine if PAH could be used as an indicator of combustion-derived sulfate deposition. Comparisons between regions indicated that the Adirondacks have a significantly higher burden of PAH than do northern New England, the northern Great Lakes States, and northern Florida. This difference likely results from significant upwind PAH sources to the Adirondack lakes. Detailed investigation of the largest lake in the study set, Big Moose Lake, indicates that PAH may serve as conservative, combustion indicators in large lakes. In this lake, PAH fluxes and concentrations were significantly correlated with historical S emission rates. These data suggest that PAH measured in sediment cores from large lakes can serve as indicators of past combustion production deposition.

  1. Accumulation of polycyclic aromatic hydrocarbons in acid sensitive lakes

    NASA Astrophysics Data System (ADS)

    Furlong, Edward T.; Cessar, Linda Roll; Hites, Ronald A.

    1987-11-01

    Polycyclic aromatic hydrocarbon concentrations and fluxes were measured in 210Pb dated sediment cores taken from nine lakes in four regions identified as susceptible to acidification. Calculated PAH accumulations were compared with historic S emissions, accumulation of sedimentary S and anthropogenic metal accumulations to determine if PAH could be used as an indicator of combustion-derived sulfate deposition. Comparisons between regions indicated that the Adirondacks have a significantly higher burden of PAH than do northern New England, the northern Great Lakes States and northern Florida. This difference likely results from significant upwind PAH sources to the Adirondack lakes. Detailed investigation of the largest lake in the study set, Big Moose Lake, indicates that PAH may serve as conservative, combustion indicators in large lakes. In this lake, PAH fluxes and concentrations were significantly correlated with historical S emission rates. These data suggest that PAH measured in sediment cores from large lakes can serve as indicators of past combustion product deposition.

  2. Heavy metals accumulation in wood tissues of the forest-forming species growed in the Steppe technogenic landscapes in Ukraine

    NASA Astrophysics Data System (ADS)

    Lovinska, Viktoriia; Wiche, Oliver

    2016-04-01

    . Nickel maximum accumulation in black locust was fixed for the youngest copies. The same tendency was observed for Scots pine in copies of 45 years old. We did not see relation with age for mangan content in both studied species. The absolute value of the amount of mangan for Scots pine is much higher comparatevelly to black locust.

  3. Significant accumulation of nitrate in Chinese semi-humid croplands

    PubMed Central

    Zhou, Junyu; Gu, Baojing; Schlesinger, William H.; Ju, Xiaotang

    2016-01-01

    Soil nitrate is important for crop growth, but it can also leach to groundwater causing nitrate contamination, a threat to human health. Here, we report a significant accumulation of soil nitrate in Chinese semi-humid croplands based upon more than 7000 samples from 141 sites collected from 1994 to 2015. In the 0–4 meters depth of soil, total nitrate accumulation reaches 453 ± 39, 749 ± 75, 1191 ± 89, 1269 ± 114, 2155 ± 330 kg N ha−1 on average in wheat, maize, open-field vegetables (OFV), solar plastic-roofed greenhouse vegetables (GHV) and orchard fields, respectively. Surprisingly, there is also a comparable amount of nitrate accumulated in the vadose-zone deeper than 4 meters. Over-use of N fertilizer (and/or manure) and a declining groundwater table are the major causes for this huge nitrate reservoir in the vadose-zone of semi-humid croplands, where the nitrate cannot be denitrified due to the presence of oxygen and lack of carbon sources. Future climatic change with more extreme rainfall events would increase the risk of accumulated nitrate moving downwards and threatening groundwater nitrate contamination. PMID:27114032

  4. Significant accumulation of nitrate in Chinese semi-humid croplands

    NASA Astrophysics Data System (ADS)

    Zhou, Junyu; Gu, Baojing; Schlesinger, William H.; Ju, Xiaotang

    2016-04-01

    Soil nitrate is important for crop growth, but it can also leach to groundwater causing nitrate contamination, a threat to human health. Here, we report a significant accumulation of soil nitrate in Chinese semi-humid croplands based upon more than 7000 samples from 141 sites collected from 1994 to 2015. In the 0–4 meters depth of soil, total nitrate accumulation reaches 453 ± 39, 749 ± 75, 1191 ± 89, 1269 ± 114, 2155 ± 330 kg N ha‑1 on average in wheat, maize, open-field vegetables (OFV), solar plastic-roofed greenhouse vegetables (GHV) and orchard fields, respectively. Surprisingly, there is also a comparable amount of nitrate accumulated in the vadose-zone deeper than 4 meters. Over-use of N fertilizer (and/or manure) and a declining groundwater table are the major causes for this huge nitrate reservoir in the vadose-zone of semi-humid croplands, where the nitrate cannot be denitrified due to the presence of oxygen and lack of carbon sources. Future climatic change with more extreme rainfall events would increase the risk of accumulated nitrate moving downwards and threatening groundwater nitrate contamination.

  5. Accumulation of silver nanoparticles by cultured primary brain astrocytes

    NASA Astrophysics Data System (ADS)

    Luther, Eva M.; Koehler, Yvonne; Diendorf, Joerg; Epple, Matthias; Dringen, Ralf

    2011-09-01

    Silver nanoparticles (AgNP) are components of various food industry products and are frequently used for medical equipment and materials. Although such particles enter the vertebrate brain, little is known on their biocompatibility for brain cells. To study the consequences of an AgNP exposure of brain cells we have treated astrocyte-rich primary cultures with polyvinylpyrrolidone (PVP)-coated AgNP. The incubation of cultured astrocytes with micromolar concentrations of AgNP for up to 24 h resulted in a time- and concentration-dependent accumulation of silver, but did not compromise the cell viability nor lower the cellular glutathione content. In contrast, the incubation of astrocytes for 4 h with identical amounts of silver as AgNO3 already severely compromised the cell viability and completely deprived the cells of glutathione. The accumulation of AgNP by astrocytes was proportional to the concentration of AgNP applied and significantly lowered by about 30% in the presence of the endocytosis inhibitors chloroquine or amiloride. Incubation at 4 °C reduced the accumulation of AgNP by 80% compared to the values obtained for cells that had been exposed to AgNP at 37 °C. These data demonstrate that viable cultured brain astrocytes efficiently accumulate PVP-coated AgNP in a temperature-dependent process that most likely involves endocytotic pathways.

  6. Significant accumulation of nitrate in Chinese semi-humid croplands.

    PubMed

    Zhou, Junyu; Gu, Baojing; Schlesinger, William H; Ju, Xiaotang

    2016-01-01

    Soil nitrate is important for crop growth, but it can also leach to groundwater causing nitrate contamination, a threat to human health. Here, we report a significant accumulation of soil nitrate in Chinese semi-humid croplands based upon more than 7000 samples from 141 sites collected from 1994 to 2015. In the 0-4 meters depth of soil, total nitrate accumulation reaches 453 ± 39, 749 ± 75, 1191 ± 89, 1269 ± 114, 2155 ± 330 kg N ha(-1) on average in wheat, maize, open-field vegetables (OFV), solar plastic-roofed greenhouse vegetables (GHV) and orchard fields, respectively. Surprisingly, there is also a comparable amount of nitrate accumulated in the vadose-zone deeper than 4 meters. Over-use of N fertilizer (and/or manure) and a declining groundwater table are the major causes for this huge nitrate reservoir in the vadose-zone of semi-humid croplands, where the nitrate cannot be denitrified due to the presence of oxygen and lack of carbon sources. Future climatic change with more extreme rainfall events would increase the risk of accumulated nitrate moving downwards and threatening groundwater nitrate contamination. PMID:27114032

  7. Zinc and dexamethasone induce metallothionein accumulation by endothelial cells

    SciTech Connect

    Briske-Anderson, M.; Bobilya, D.J.; Reeves, P.G. )

    1991-03-11

    Several tissues increase their metallothionein (MT) concentration when exposed to elevated amounts of plasma Zn. Endothelial cells form the blood vessels that supply all tissues and constitute a barrier between cells of tissues and the blood. This study examined the ability of endothelial cells to synthesize MT and accumulate Zn in response to high amounts of Zn and dexamethasone. Bovine pulmonary endothelial cells were grown to confluence in Minimum Essential Medium with Earle's salts and 10% fetal calf serum. The monolayer was maintained for 2 d prior to use in medium containing EDTA-dialyzed serum. This low Zn medium was replaced with one containing 1, 6, 25, 50, 100, 150, or 200 {mu}M Zn and incubated for 24 hr before harvesting the cells. MT was quantified by the cadmium binding assay. Cellular Zn concentrations were analyzed by atomic absorption after a nitric acid digestion. The MT concentration was elevated in response to Zn concentrations of 100 {mu}M or more. Cellular Zn concentration was elevated when media Zn was 25 {mu}M or more. MT and cellular Zn concentrations were positively correlated. In another study, inclusion of 0.1 {mu}M dexamethasone in the media increased concentration at all Zn concentrations studied. However, the inclusion of 0.3 {mu}M cis-platinum had no effect. In conclusion, endothelial cells in culture respond to elevated amounts of Zn and dexamethasone in the media by accumulating Zn and MT.

  8. Higher Education: A Time for Triage?

    NASA Astrophysics Data System (ADS)

    Lagowski, J. J.

    1995-10-01

    is replete with descriptions of budget cuts and the resultant reallocation of monies. For example, as the budget cuts of the last decade accumulated, maintenance was deferred, and the funds saved were used to shore-up key existing parts of the educational process, such as faculty salaries. State budgets are generally smaller now than they were when the cuts were made, which means that preventive maintenance will continue to be deferred and other resources must be found for capital improvements. Triage often operates in an environment that does not permit promising possibilities to develop. For example, the promise of interactive digital technologies on the learning process may never be fully realized in many institutions if the associated capital and operating costs cannot be accommodated within the cost containment measures being adopted. In an effort to offset part of the lost state support, tuition and fees have been increased at public institutions at a rate that far exceeds growth of the cost of living index. All this is occurring in the face of an increasingly diverse student body and the beginning of "Tidal Wave II," as the surge of new students who are the children of the baby boomers has been called. These demands, along with the expectations for an historically, good American education, will have to be met with fewer dollars. Our ability to fund public higher education by the conventional mechanisms has been affected by a variety of tax reform initiatives. Although the details may vary locally, various kinds of initiatives, propositions, and referenda have severely limited the amount of revenue states can raise. Thus, caps on property taxes have transformed support patterns at the city and county levels. Initiatives, many of which have built-in escalators, that fix the percentages of state spending for various programs have created new kinds of budgetary entitlement groups. These mandates conspire to give government, i.e., governors and legislatures, less

  9. Proteomics Profiling Reveals Carbohydrate Metabolic Enzymes and 14-3-3 Proteins Play Important Roles for Starch Accumulation during Cassava Root Tuberization.

    PubMed

    Wang, Xuchu; Chang, Lili; Tong, Zheng; Wang, Dongyang; Yin, Qi; Wang, Dan; Jin, Xiang; Yang, Qian; Wang, Liming; Sun, Yong; Huang, Qixing; Guo, Anping; Peng, Ming

    2016-01-01

    Cassava is one of the most important root crops as a reliable source of food and carbohydrates. Carbohydrate metabolism and starch accumulation in cassava storage root is a cascade process that includes large amounts of proteins and cofactors. Here, comparative proteomics were conducted in cassava root at nine developmental stages. A total of 154 identified proteins were found to be differentially expressed during starch accumulation and root tuberization. Many enzymes involved in starch and sucrose metabolism were significantly up-regulated, and functional classification of the differentially expressed proteins demonstrated that the majority were binding-related enzymes. Many proteins were took part in carbohydrate metabolism to produce energy. Among them, three 14-3-3 isoforms were induced to be clearly phosphorylated during storage root enlargement. Overexpression of a cassava 14-3-3 gene in Arabidopsis thaliana confirmed that the older leaves of these transgenic plants contained higher sugar and starch contents than the wild-type leaves. The 14-3-3 proteins and their binding enzymes may play important roles in carbohydrate metabolism and starch accumulation during cassava root tuberization. These results not only deepened our understanding of the tuberous root proteome, but also uncovered new insights into carbohydrate metabolism and starch accumulation during cassava root enlargement. PMID:26791570

  10. Proteomics Profiling Reveals Carbohydrate Metabolic Enzymes and 14-3-3 Proteins Play Important Roles for Starch Accumulation during Cassava Root Tuberization

    PubMed Central

    Wang, Xuchu; Chang, Lili; Tong, Zheng; Wang, Dongyang; Yin, Qi; Wang, Dan; Jin, Xiang; Yang, Qian; Wang, Liming; Sun, Yong; Huang, Qixing; Guo, Anping; Peng, Ming

    2016-01-01

    Cassava is one of the most important root crops as a reliable source of food and carbohydrates. Carbohydrate metabolism and starch accumulation in cassava storage root is a cascade process that includes large amounts of proteins and cofactors. Here, comparative proteomics were conducted in cassava root at nine developmental stages. A total of 154 identified proteins were found to be differentially expressed during starch accumulation and root tuberization. Many enzymes involved in starch and sucrose metabolism were significantly up-regulated, and functional classification of the differentially expressed proteins demonstrated that the majority were binding-related enzymes. Many proteins were took part in carbohydrate metabolism to produce energy. Among them, three 14-3-3 isoforms were induced to be clearly phosphorylated during storage root enlargement. Overexpression of a cassava 14-3-3 gene in Arabidopsis thaliana confirmed that the older leaves of these transgenic plants contained higher sugar and starch contents than the wild-type leaves. The 14-3-3 proteins and their binding enzymes may play important roles in carbohydrate metabolism and starch accumulation during cassava root tuberization. These results not only deepened our understanding of the tuberous root proteome, but also uncovered new insights into carbohydrate metabolism and starch accumulation during cassava root enlargement. PMID:26791570

  11. The role of the equatorial ligands for the redox behavior, mode of cellular accumulation and cytotoxicity of platinum(IV) prodrugs.

    PubMed

    Göschl, Simone; Varbanov, Hristo P; Theiner, Sarah; Jakupec, Michael A; Galanski, Markus; Keppler, Bernhard K

    2016-07-01

    The current study aims to elucidate the possible reasons for the significantly different pharmacological behavior of platinum(IV) complexes with cisplatin-, carboplatin- or nedaplatin-like cores and how this difference can be related to their main physicochemical properties. Chlorido-containing complexes are reduced fast (within hours) by ascorbate and are able to unwind plasmid DNA in the presence of ascorbate, while their tri- and tetracarboxylato analogs are generally inert under the same conditions. Comparison of the lipophilicity, cellular accumulation and cytotoxicity of the investigated platinum compounds revealed the necessity to define new structure-property/activity relationships (SPRs and SARs). The higher activity and improved accumulation of platinum(IV) complexes bearing Cl(-) in equatorial position cannot only be attributed to passive diffusion facilitated by their lipophilicity. Therefore, further platinum accumulation experiments under conditions where active/facilitated transport mechanisms are suppressed were performed. Under hypothermic conditions (4°C), accumulation of dichloridoplatinum(IV) complexes is reduced down to 10% of the amount determined at 37°C. These findings suggest the involvement of active and/or facilitated transport in cellular uptake of platinum(IV) complexes with a cisplatin-like core. Studies with ATP depletion mediated by oligomycin and low glucose partially confirmed these observations, but their feasibility was severely limited in the adherent cell culture setting. PMID:27055943

  12. Bioavailability of Fullerene under Environmentally Relevant Conditions: Effects of Humic Acid and Fetal Bovine Serum on Accumulation in Lipid Bilayers and Cellular Uptake.

    PubMed

    Ha, Yeonjeong; Wang, Xianzhe; Liljestrand, Howard M; Maynard, Jennifer A; Katz, Lynn E

    2016-07-01

    Carbon fullerene (C60) has emerged at the forefront of nanoscale research and application due to its unique properties. As the production of this nanoparticle rapidly increases, it can be released into natural aquatic environments and can accumulate in biological systems. This research examined the effects of humic acid and fetal bovine serum (FBS), which are ubiquitous in aquatic environments and representative of blood plasma in living organisms, respectively, on bioavailability of fullerene. Bioavailability was investigated using in vitro methods for lipid membrane accumulation and cellular uptake studies. Humic acid and FBS significantly changed the characteristics of fullerene including its particle size and surface charge. The effects of humic acid on lipid accumulation of fullerene depended on the lipid head charge. FBS also significantly decreased the lipid accumulation when positively charged and zwitterionic head groups were present on the lipids, possibly due to the higher steric repulsion of the protein coated nanoparticles. In addition, both humic acid and FBS protein effectively lowered the amounts of fullerene taken up by Caco-2 cells, which are derived from a human colorectal adenocarcinoma and have similar functions to the small intestinal epithelium. Results of this study suggest that surface modification of fullerene by environmentally relevant matrices can significantly affect the biological transport, as well as the possible toxicity of this nanomaterial. PMID:26943027

  13. ACCUMULATION OF METHYLMERCURY OR POLYCHLORINATED BIPHENYLS IN IN VITRO MODELS OF RAT NEURONAL TISSUE.

    EPA Science Inventory

    This manuscript reports data examining the accumulation of PCBs or methylmercury into the tissue of three commonly used in vitro neuronal models.

    ? The results demonstrate that these lipophilic compounds can accumulate to levels 5 to 100 fold higher than the surrounding s...

  14. Site of Fluoride Accumulation in Navel Orange Leaves 1

    PubMed Central

    Chang, Chong W.; Thompson, C. Ray

    1966-01-01

    Fluoride-polluted navel orange leaves, Citrus sinensis (Linn.) Osbeck, were fractionated into the subcellular components in hexane/carbon tetrachloride mixtures having various densities. Fluoride was determined at each fraction. Analyses were also made for the subcellular distribution of chlorophyll, nitrogen, and DNA to assess the extent of cross-contamination of each component. The fraction containing cell wall, nuclei, and partly broken cells apparently contained a major amount of fluoride. However, if allowance was made for the cross-contamination of chloroplasts and chloroplast fragments, the fraction of chloroplasts was found to be the site of the highest fluoride accumulation. When each particulate component was washed with water after drying, the combined washings contained more than 50% of the total fluoride of the isolated fractions. The usual method of subcellular fractionation with aqueous solvent shifted the major site of fluoride accumulation from the fraction of chloroplasts to that of the supernatant. PMID:5908632

  15. Atomistic simulation of damage accumulation and amorphization in Ge

    SciTech Connect

    Gomez-Selles, Jose L. Martin-Bragado, Ignacio; Claverie, Alain; Benistant, Francis

    2015-02-07

    Damage accumulation and amorphization mechanisms by means of ion implantation in Ge are studied using Kinetic Monte Carlo and Binary Collision Approximation techniques. Such mechanisms are investigated through different stages of damage accumulation taking place in the implantation process: from point defect generation and cluster formation up to full amorphization of Ge layers. We propose a damage concentration amorphization threshold for Ge of ∼1.3 × 10{sup 22} cm{sup −3} which is independent on the implantation conditions. Recombination energy barriers depending on amorphous pocket sizes are provided. This leads to an explanation of the reported distinct behavior of the damage generated by different ions. We have also observed that the dissolution of clusters plays an important role for relatively high temperatures and fluences. The model is able to explain and predict different damage generation regimes, amount of generated damage, and extension of amorphous layers in Ge for different ions and implantation conditions.

  16. Elevation changes in Antarctica mainly determined by accumulation variability.

    PubMed

    Helsen, Michiel M; van den Broeke, Michiel R; van de Wal, Roderik S W; van de Berg, Willem Jan; van Meijgaard, Erik; Davis, Curt H; Li, Yonghong; Goodwin, Ian

    2008-06-20

    Antarctic Ice Sheet elevation changes, which are used to estimate changes in the mass of the interior regions, are caused by variations in the depth of the firn layer. We quantified the effects of temperature and accumulation variability on firn layer thickness by simulating the 1980-2004 Antarctic firn depth variability. For most of Antarctica, the magnitudes of firn depth changes were comparable to those of observed ice sheet elevation changes. The current satellite observational period ( approximately 15 years) is too short to neglect these fluctuations in firn depth when computing recent ice sheet mass changes. The amount of surface lowering in the Amundsen Sea Embayment revealed by satellite radar altimetry (1995-2003) was increased by including firn depth fluctuations, while a large area of the East Antarctic Ice Sheet slowly grew as a result of increased accumulation. PMID:18511656

  17. Nutrient-contaminant (Pu) plant accumulation model

    SciTech Connect

    Cowan, C.E.; Jenne, E.A.; Simpson, J.C.; Cataldo, D.A.

    1981-12-01

    A model was developed which simulates the movement and daily accumulation of nutrients and contaminants in crop plants resulting from known physiological processes in the plant. In the model, the daily contaminant accumulation is governed by daily increase in plant biomass derived from photosynthesis and by the specified thermodynamic activity of the bioavailable contaminant species in soil or hydroponic solutin. Total accumulation and resulting concentration in the plant's root, stem and branch, leaf, and reproductive compartments can be simulated any time during the growing season. Parameters were estimated from data on plutonium accumulation in soybeans and the model was calibrated against this same data set. The plutonium distribution in the plant was found to be most sensitive to parameters related to leaf accumulation. Contamination at different times during the growing season resulted in a large change in predicted leaf accumulation but very little change in predicted accumulation in other plant parts except when contamination occurred very late in the growing season.

  18. Lysosomal Cholesterol Accumulation Sensitizes To Acetaminophen Hepatotoxicity by Impairing Mitophagy

    PubMed Central

    Baulies, Anna; Ribas, Vicent; Núñez, Susana; Torres, Sandra; Alarcón-Vila, Cristina; Martínez, Laura; Suda, Jo; Ybanez, Maria D.; Kaplowitz, Neil; García-Ruiz, Carmen; Fernández-Checa, Jose C.

    2015-01-01

    The role of lysosomes in acetaminophen (APAP) hepatotoxicity is poorly understood. Here, we investigated the impact of genetic and drug-induced lysosomal cholesterol (LC) accumulation in APAP hepatotoxicity. Acid sphingomyelinase (ASMase)−/− mice exhibit LC accumulation and higher mortality after APAP overdose compared to ASMase+/+ littermates. ASMase−/− hepatocytes display lower threshold for APAP-induced cell death and defective fusion of mitochondria-containing autophagosomes with lysosomes, which decreased mitochondrial quality control. LC accumulation in ASMase+/+ hepatocytes caused by U18666A reproduces the susceptibility of ASMase−/− hepatocytes to APAP and the impairment in the formation of mitochondria-containing autolysosomes. LC extraction by 25-hydroxycholesterol increased APAP-mediated mitophagy and protected ASMase−/− mice and hepatocytes against APAP hepatotoxicity, effects that were reversed by chloroquine to disrupt autophagy. The regulation of LC by U18666A or 25-hydroxycholesterol did not affect total cellular sphingomyelin content or its lysosomal distribution. Of relevance, amitriptyline-induced ASMase inhibition in human hepatocytes caused LC accumulation, impaired mitophagy and increased susceptibility to APAP. Similar results were observed upon glucocerebrosidase inhibition by conduritol β-epoxide, a cellular model of Gaucher disease. These findings indicate that LC accumulation determines susceptibility to APAP hepatotoxicity by modulating mitophagy, and imply that genetic or drug-mediated ASMase disruption sensitizes to APAP-induced liver injury. PMID:26657973

  19. Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation

    SciTech Connect

    Nawrath, C.; Poirier, Y.; Somerville, C. )

    1994-12-20

    In the bacterium Alcaligenes eutrophus, three genes encode the enzymes necessary to catalyze the synthesis of poly[(R)-(-)-3-hydroxybutyrate] (PHB) from acetyl-CoA. In order to target these enzymes into the plastids of higher plants, the genes were modified by addition of DNA fragments encoding a pea chloroplast transit peptide, a constitutive plant promoter, and a poly(A) addition sequence. Each of the modified bacterial genes was introduced into Arabidopsis thaliana by Agrobacterium-mediated transformation, and plants containing all three genes were obtained by sexual crosses. These plans accumulated PHB up to 14% of the dry weight as 0.2- to 0.7-[mu]m granules within plastids. In contrast to earlier experiments in which expression of the PHB biosynthetic pathway in the cytoplasm led to a deleterious effect on growth, expression of the PHB biosynthetic pathway in plastids had no obvious effect on the growth or fertility of the transgenic plants and resulted in a 100-fold increase in the amount of PHB in higher plants. The high level of PHB accumulation also suggests that the synthesis of plastid acetyl-CoA is regulated by a mechanism which responds to metabolic demand. 20 refs., 6 figs.

  20. Oxidative DNA damage accumulation in gastric carcinogenesis

    PubMed Central

    Farinati, F; Cardin, R; Degan, P; Rugge, M; Di, M; Bonvicini, P; Naccarato, R

    1998-01-01

    Background—Gastric carcinogenesis is a multifactorial, multistep process, in which chronic inflammation plays a major role. 
Aims—In order to ascertain whether free radical mediated oxidative DNA damage is involved in such a process, concentrations of 8-hydroxydeoxyguanosine (8OHdG), a mutagenic/carcinogenic adduct, and thiobarbituric acid reactive substances (TBARS), as an indirect measure of free radical mediated damage, were determined in biopsy specimens from patients undergoing endoscopy. 
Patients—Eighty eight patients were divided into histological subgroups as follows: 27 with chronic non-atrophic gastritis, 41 with atrophic gastritis, six with gastric cancer, and 14 unaffected controls. 
Methods—Intestinal metaplasia, Helicobacter pylori infection, and disease activity were semiquantitatively scored. 8OHdG concentrations were assessed by HPLC with electrochemical detection, and TBARS concentrations were fluorimetrically assayed. 
Results—8OHdG concentrations (mean number of adducts/105 dG residues) were significantly higher in chronic atrophic gastritis (p=0.0009). Significantly higher concentrations were also detected in the presence of severe disease activity (p=0.02), intestinal metaplasia (p=0.035), and H pylori infection (p=0.001). TBARS concentrations were also higher in atrophic gastritis, though not significantly so. In a multiple logistic regression analysis, 8OHdG concentrations correlated best with the presence and severity of H pylori infection (r=0.53, p=0.002). 
Conclusions—Chronic gastritis is characterised by the accumulation of oxidative DNA damage with mutagenic and carcinogenic potential. H pylori infection is the major determinant for DNA adduct formation. 

 Keywords: free radicals; oxidative DNA damage; gastric carcinogenesis; precancerous changes; peroxidative damage PMID:9577340

  1. Higher Education Exchange, 2014

    ERIC Educational Resources Information Center

    Brown, David W., Ed.; Witte, Deborah, Ed.

    2014-01-01

    Research shows that not only does higher education not see the public; when the public, in turn, looks at higher education, it sees mostly malaise, inefficiencies, expense, and unfulfilled promises. Yet, the contributors to this issue of the "Higher Education Exchange" tell of bright spots in higher education where experiments in working…

  2. Higher Education Exchange, 2008

    ERIC Educational Resources Information Center

    Brown, David W., Ed.; Witte, Deborah, Ed.

    2008-01-01

    "Higher Education Exchange" publishes case studies, analyses, news, and ideas about efforts within higher education to develop more democratic societies. Contributors to this issue of the "Higher Education Exchange" examine whether institutions of higher learning are doing anything to increase the capacity of citizens to shape their future.…

  3. Higher Education Exchange, 2010

    ERIC Educational Resources Information Center

    Brown, David W., Ed.; Witte, Deborah, Ed.

    2010-01-01

    "Higher Education Exchange" publishes case studies, analyses, news, and ideas about efforts within higher education to develop more democratic societies. Contributors to this issue of the "Higher Education Exchange" examine whether institutions of higher learning are doing anything to increase the capacity of citizens to shape their future.…

  4. Higher Education Exchange, 2011

    ERIC Educational Resources Information Center

    Brown, David W., Ed.; Witte, Deborah, Ed.

    2011-01-01

    "Higher Education Exchange" publishes case studies, analyses, news, and ideas about efforts within higher education to develop more democratic societies. Contributors to this issue of the "Higher Education Exchange" examine whether institutions of higher learning are doing anything to increase the capacity of citizens to shape their future.…

  5. Higher Education Exchange, 2012

    ERIC Educational Resources Information Center

    Brown, David W., Ed.; Witte, Deborah, Ed.

    2012-01-01

    "Higher Education Exchange" publishes case studies, analyses, news, and ideas about efforts within higher education to develop more democratic societies. Contributors to this issue of the "Higher Education Exchange" examine whether institutions of higher learning are doing anything to increase the capacity of citizens to shape their future.…

  6. Plants accumulating heavy metals in the Danube River wetlands

    PubMed Central

    2013-01-01

    Background We present herein our results regarding the accumulation of four heavy metals (copper, cadmium, lead, and zinc) in four aquatic species plants (Ceratophyllum demersum, Potamogeton pectinatus, Potamogeton lucens, Potamogeton perfoliatus) collected from the Danube River, South-Western part of Romania and their possible use as indicators of aquatic ecosystems pollution with heavy metals. Methods Elements concentration from the vegetal material was determined through Inductively Coupled Plasma – Mass Spectrometry. Results The species were chosen based on their previous use as bioindicators in aquatic ecosystems and due to the fact they are one of the most frequent aquatic plant species of the Danube River ecosystems within the Iron Gates Natural Park. Highest amounts are recorded for Ceratophyllum demersum (3.52 μg/g for Cd; 22.71 μg/g for Cu; 20.06 μg/g for Pb; 104.23 μg/g for Zn). Among the Potamogeton species, the highest amounts of heavy metals are recorded in Potamogeton perfoliatus (1.88 μg/g for Cd; 13.14 μg/g for Cu; 13.32 μg/g for Pb; 57.96 μg/g for Zn). The sequence for the bioconcentration factors (BCFs) calculated in order to describe the accumulation of the four metals is Cd >> Zn > Pb > Cu. Increase of the zinc concentration determines an increase of the cadmium concentration (Spearman rho=0.40, p=0.02). Conclusions Despite the low ambiental levels of heavy metals, the four aquatic plants have the ability to accumulate significant amounts, which make them useful as biological indicators. BCF value for Ceratophyllum demersum indicated this species as a cadmium hyperaccumulator. PMID:24359799

  7. Characterization of Cd translocation and accumulation in 19 maize cultivars grown on Cd-contaminated soil: implication of maize cultivar selection for minimal risk to human health and for phytoremediation.

    PubMed

    Wang, Aiyun; Wang, Minyan; Liao, Qi; He, Xiquan

    2016-03-01

    Maize (Zea mays) has low Cd accumulation in grains and a high biomass compared to other crops. The capacities for Cd accumulation in different maize cultivars are, however, not fully understood. To reduce human health risk from maize grown in Cd-contaminated soil and to provide promising maize cultivars for the phytoremediation of Cd-polluted soil, a field experiment was conducted to screen low-Cd- and high-Cd-accumulation maize cultivars by evaluating the yield, Cd uptake, translocation, and accumulation differences among 19 maize cultivars. There were differences in straw dry weight (DW), root DW, and yield among the 19 cultivars. The cultivars Yudan19, Zhengda999, and Xianyu508 had a higher production compared to that of the other cultivars. The Cd concentrations in the roots were much higher than those in the straws and grains in all cultivars. The Cd accumulation factors (AFS) decreased in the order of accumulation factors in root (AFrs) > accumulation factors in straw (AFss) > accumulation factors in grain (AFgs). The Cd translocation factors (TFs) from root to straw (TFrs) were significantly (p < 0.05) larger than those from straw to grain (TFsg) among all of the cultivars. The TFs for all of the cultivars was less than 1, and the lowest TFsg (0.23) was found in cultivar Xiangyongdan3. The correlation analysis indicated that Cd concentrations in straws showed a significant (p < 0.01) as well as positive correlation with TFrs while a negative correlation with TFsg (p < 0.01). Moreover, Cd accumulation in different tissues decreased in the order straw > grain > root. Among the 19 maize cultivars, Jixiang2118 and Kangnong18 accumulated the highest Cd amount in the aboveground tissues, and the corresponding values were 7,206.51 and 6,598.68 mg hm(-2), respectively. A hierarchical cluster analysis based on the Cd concentrations in grains and straws classified the 19 maize cultivars into four and two groups for a 0.4 minimum distance between clusters

  8. Factors Influencing the Accumulation and Subsurface Transport of Fecal Indicator Bacteria near the Shoreline at Freshwater Beaches

    NASA Astrophysics Data System (ADS)

    Wu, M. Z.; O'Carroll, D. M.; Vogel, L. J.; Robinson, C. E.

    2015-12-01

    Beach sand near the shoreline acts as a reservoir for fecal contaminants with fecal indicator bacteria (FIB) often orders of magnitude higher than in adjacent surface waters. This reservoir poses a human health risk and can also act as an important non-point contamination source for surface waters. Beach water quality advisories or closures can be issued when FIB (Escherichia coli (E. coli), enterococci (ENT)) concentrations are elevated in the surface water. The factors controlling the transport and accumulation of FIB in the foreshore sand are not well understood, though this is required to manage and mitigate this source. Multiple sources may contribute to the accumulation of FIB in sand, with recent studies suggesting that the continuous influx of surface water across the sediment-water interface may be a dominant source at many beaches.The study objective was to develop understanding of the physical processes controlling the accumulation and transport of FIB in beach sand. Field measurements were combined with numerical modelling to evaluate the role of low-energy lapping waves in delivering FIB to the saturated foreshore sand at freshwater beaches. E. coli and ENT were measured at two beaches in Ontario, Canada at depths of up to 1 and 2 m, respectively, below the water table. A numerical model simulating wave-induced groundwater recirculations coupled with microbial transport (using colloid filtration theory) showed that the different FIB distributions measured at the two beaches was due mainly to the different beach slope and terrestrial groundwater flow. The model was applied to assess the impact of beach, wave and bacterial parameters on FIB accumulation. The infiltration zone width, average infiltration velocity and infiltration rate were shown to ultimately control the amount and spatial distribution of FIB in the sand. The study findings are important in understanding factors controlling the transport of FIB at the sediment-water interface of

  9. Natural radionuclide accumulation by raindrops

    NASA Astrophysics Data System (ADS)

    Gusev, Anatoly; Martin, Inacio; Shkevov, Rumen; Alves, Mauro

    2016-07-01

    The laboratory of environmental radiation of ITA (São José dos Campos, 23°11'11″S, 45°52'43″W, 650 MAMSL) performs simultaneous monitoring of a natural radiation background and meteorological parameters. A time resolution of up to 1 minute allows a detailed comparison of changes in meteorological parameters with those of a concentration of ambient radon progenies in the atmosphere. Results of a study of variation of a fallout of radon progenies ^{214}Pb and ^{214}Bi concomitanting rainfalls are present. The radionuclide fallout rate is reconstructed from the observed gamma rate through a simulation of the first kind Volterra integral equation with difference kernel, determined by ratio of precipitating rates of 214Pb and 214Bi and their decay half times. An original straightforward step-by-step procedure was used for the numerical solution of the equation. The radionuclide concentration in the rainwater is calculated as a ratio of the reconstructed fallout to the measured rainfall. It was observed that the radionuclide fallout rate increases as the rainfall one in approximately power 0.6, i.e. the same as the mean raindrop volume. The concentration thereafter decreases as the rainfall rate in power 0.4. A numerical simulation of the process of accumulation of the radionuclides during diffusion and coalescence drop growth and aerosol scavenging during a passage from a cloud to the ground was performed. The results of the simulations agree with the experimental data.

  10. Accumulation of pyrethroid compounds in primary cultures of rat cortical neurons

    EPA Science Inventory

    Recent studies have demonstrated that lipophilic compounds (e.g. methylmercury, polychlorinated biphenyls (PCBs) and polybrominated diphenylethers (PBDEs)) rapidly accumulate in cells in culture to concentrations much higher than in the surrounding media. Primary cultures of neur...

  11. Assessing the Capacity of Plant Species to Accumulate Particulate Matter in Beijing, China

    PubMed Central

    Mo, Li; Ma, Zeyu; Xu, Yansen; Sun, Fengbin; Lun, Xiaoxiu; Liu, Xuhui; Chen, Jungang; Yu, Xinxiao

    2015-01-01

    Air pollution causes serious problems in spring in northern China; therefore, studying the ability of different plants to accumulate particulate matter (PM) at the beginning of the growing season may benefit urban planners in their attempts to control air pollution. This study evaluated deposits of PM on the leaves and in the wax layer of 35 species (11 shrubs, 24 trees) in Beijing, China. Differences in the accumulation of PM were observed between species. Cephalotaxus sinensis, Euonymus japonicus, Broussonetia papyriferar, Koelreuteria paniculata and Quercus variabilis were all efficient in capturing small particles. The plants exhibiting high amounts of total PM accumulation (on leaf surfaces and/or in the wax layer), also showed comparatively high levels of PM accumulation across all particle sizes. A comparison of shrubs and trees did not reveal obvious differences in their ability to accumulate particles based on growth form; a combination of plantings with different growth forms can efficiently reduce airborne PM concentrations near the ground. To test the relationships between leaf traits and PM accumulation, leaf samples of selected species were observed using a scanning electron microscope. Growth forms with greater amounts of pubescence and increased roughness supported PM accumulation; the adaxial leaf surfaces collected more particles than the abaxial surfaces. The results of this study may inform the selection of species for urban green areas where the goal is to capture air pollutants and mitigate the adverse effects of air pollution on human health. PMID:26506104

  12. 24 CFR 84.80 - Conditions for use of Lump Sum (fixed price or fixed amount) grants.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... (fixed price or fixed amount) grants. 84.80 Section 84.80 Housing and Urban Development Office of the... AGREEMENTS WITH INSTITUTIONS OF HIGHER EDUCATION, HOSPITALS, AND OTHER NON-PROFIT ORGANIZATIONS Use of Lump Sum Grants § 84.80 Conditions for use of Lump Sum (fixed price or fixed amount) grants. (a) Heads...

  13. Exploring metal detoxification and accumulation potential during vermicomposting of Tea factory coal ash: sequential extraction and fluorescence probe analysis

    PubMed Central

    Goswami, Linee; Pratihar, Sanjay; Dasgupta, Suman; Bhattacharyya, Pradip; Mudoi, Pronab; Bora, Jayanta; Bhattacharya, Satya Sundar; Kim, Ki Hyun

    2016-01-01

    Metal contamination from coal ashes (CAs) is widely recognized as a significant environmental concern. To learn more about metal detoxification and accumulation potential of earthworm species, metal-rich tea factory coal ashes (TFCA) were fed to Eisenia fetida and Lampito mauritii by employing a fluorescent tag detection method. Fascinatingly, on feeding fluorescence probed Zn and Cd along with cow dung to Eisenia fetida, the detection of the gut-proteins with a molecular mass higher than 100 kDa was a distinct evidence of metal binding. Significant increases were observed in the content of humified organic C [humic acid (HAC) and fulvic acid C (FAC)] and degree of humification during vermicomposting. Concurrently, considerably large amount of toxic metals (Cr, Cd, Pb, and Zn) was transformed from exchangeable to recalcitrant (organic matter and mineral bound) fractions. Moreover, total metal concentrations were reduced with high removal efficiency upon vermicomposting. PMID:27456167

  14. Exploring metal detoxification and accumulation potential during vermicomposting of Tea factory coal ash: sequential extraction and fluorescence probe analysis

    NASA Astrophysics Data System (ADS)

    Goswami, Linee; Pratihar, Sanjay; Dasgupta, Suman; Bhattacharyya, Pradip; Mudoi, Pronab; Bora, Jayanta; Bhattacharya, Satya Sundar; Kim, Ki Hyun

    2016-07-01

    Metal contamination from coal ashes (CAs) is widely recognized as a significant environmental concern. To learn more about metal detoxification and accumulation potential of earthworm species, metal-rich tea factory coal ashes (TFCA) were fed to Eisenia fetida and Lampito mauritii by employing a fluorescent tag detection method. Fascinatingly, on feeding fluorescence probed Zn and Cd along with cow dung to Eisenia fetida, the detection of the gut-proteins with a molecular mass higher than 100 kDa was a distinct evidence of metal binding. Significant increases were observed in the content of humified organic C [humic acid (HAC) and fulvic acid C (FAC)] and degree of humification during vermicomposting. Concurrently, considerably large amount of toxic metals (Cr, Cd, Pb, and Zn) was transformed from exchangeable to recalcitrant (organic matter and mineral bound) fractions. Moreover, total metal concentrations were reduced with high removal efficiency upon vermicomposting.

  15. Exploring metal detoxification and accumulation potential during vermicomposting of Tea factory coal ash: sequential extraction and fluorescence probe analysis.

    PubMed

    Goswami, Linee; Pratihar, Sanjay; Dasgupta, Suman; Bhattacharyya, Pradip; Mudoi, Pronab; Bora, Jayanta; Bhattacharya, Satya Sundar; Kim, Ki Hyun

    2016-01-01

    Metal contamination from coal ashes (CAs) is widely recognized as a significant environmental concern. To learn more about metal detoxification and accumulation potential of earthworm species, metal-rich tea factory coal ashes (TFCA) were fed to Eisenia fetida and Lampito mauritii by employing a fluorescent tag detection method. Fascinatingly, on feeding fluorescence probed Zn and Cd along with cow dung to Eisenia fetida, the detection of the gut-proteins with a molecular mass higher than 100 kDa was a distinct evidence of metal binding. Significant increases were observed in the content of humified organic C [humic acid (HAC) and fulvic acid C (FAC)] and degree of humification during vermicomposting. Concurrently, considerably large amount of toxic metals (Cr, Cd, Pb, and Zn) was transformed from exchangeable to recalcitrant (organic matter and mineral bound) fractions. Moreover, total metal concentrations were reduced with high removal efficiency upon vermicomposting. PMID:27456167

  16. Accumulation and distribution of trace metals within soils and the austral cordgrass Spartina densiflora in a Patagonian salt marsh.

    PubMed

    Idaszkin, Yanina L; Lancelotti, Julio L; Bouza, Pablo J; Marcovecchio, Jorge E

    2015-12-15

    Concentrations of Cd, Cu, Fe, Pb, and Zn were determined in soils and in below- and above-ground structures of Spartina densiflora in a Patagonian salt marsh (San Antonio, Río Negro, Argentina). Also, the relationship between trace metal concentrations in soils and plants was investigated to improve our knowledge regarding the ability of this plant species to take up and accumulate trace metals from the soil. Our results indicate that, within the studied salt marsh, soil trace metal concentrations follow a decreasing concentration gradient toward the sea. They show moderate pollution and a potentially negative biological effect in one site of the salt marsh. While below-ground structures reflect the soil metal concentration pattern, this is not so evident in above-ground concentrations. Also, S. densiflora is able to absorb a limited amount of metals present in the soil, the soil bioaccumulation factor being lower in sites where soil metal concentration is higher. PMID:26481413

  17. 14 CFR 198.7 - Amount of insurance coverage available.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Amount of insurance coverage available. 198.7 Section 198.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) WAR RISK INSURANCE AVIATION INSURANCE § 198.7 Amount of insurance coverage available. (a)...

  18. 14 CFR 198.7 - Amount of insurance coverage available.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Amount of insurance coverage available. 198.7 Section 198.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) WAR RISK INSURANCE AVIATION INSURANCE § 198.7 Amount of insurance coverage available. (a)...

  19. 14 CFR 198.7 - Amount of insurance coverage available.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Amount of insurance coverage available. 198.7 Section 198.7 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) WAR RISK INSURANCE AVIATION INSURANCE § 198.7 Amount of insurance coverage available. (a)...

  20. 46 CFR 151.45-6 - Maximum amount of cargo.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 5 2011-10-01 2011-10-01 false Maximum amount of cargo. 151.45-6 Section 151.45-6 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Operations § 151.45-6 Maximum amount of cargo. (a) Tanks carrying liquids or liquefied gases at...

  1. 46 CFR 151.45-6 - Maximum amount of cargo.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 5 2014-10-01 2014-10-01 false Maximum amount of cargo. 151.45-6 Section 151.45-6 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CERTAIN BULK DANGEROUS CARGOES BARGES CARRYING BULK LIQUID HAZARDOUS MATERIAL CARGOES Operations § 151.45-6 Maximum amount of cargo. (a) Tanks carrying liquids or liquefied gases at...

  2. 31 CFR 50.71 - Determination of recoupment amounts.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... 50.71 Section 50.71 Money and Finance: Treasury Office of the Secretary of the Treasury TERRORISM... recoupment amounts for that Program Year. (b)(1) Within 90 days after certification of an act of terrorism... Terrorism Policy Surcharge previously established to determine whether any additional amount will...

  3. 31 CFR 50.71 - Determination of recoupment amounts.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... 50.71 Section 50.71 Money and Finance: Treasury Office of the Secretary of the Treasury TERRORISM... recoupment amounts for that Program Year. (b)(1) Within 90 days after certification of an act of terrorism... Terrorism Policy Surcharge previously established to determine whether any additional amount will...

  4. 42 CFR 51c.106 - Amount of grant.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR COMMUNITY HEALTH SERVICES General Provisions § 51c.106 Amount of grant. (a) The amount of any award under this part will be... the grantee to finance its share of project costs from non-Federal sources; (B) The need in the...

  5. 42 CFR 56.106 - Amount of grant.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR MIGRANT HEALTH SERVICES General Provisions § 56.106 Amount of grant. (a) The amount of any award under this part will be... the grantee to finance its share of project costs from non-Federal sources; (B) The need in the...

  6. 42 CFR 56.106 - Amount of grant.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR MIGRANT HEALTH SERVICES General Provisions § 56.106 Amount of grant. (a) The amount of any award under this part will be... the grantee to finance its share of project costs from non-Federal sources; (B) The need in the...

  7. 42 CFR 51c.106 - Amount of grant.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR COMMUNITY HEALTH SERVICES General Provisions § 51c.106 Amount of grant. (a) The amount of any award under this part will be... the grantee to finance its share of project costs from non-Federal sources; (B) The need in the...

  8. 43 CFR 3904.15 - Amount of bond.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... that a change in amount is appropriate to cover the costs and obligations of complying with the... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false Amount of bond. 3904.15 Section 3904.15 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND...

  9. 43 CFR 3904.15 - Amount of bond.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... that a change in amount is appropriate to cover the costs and obligations of complying with the... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false Amount of bond. 3904.15 Section 3904.15 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND...

  10. 43 CFR 3904.15 - Amount of bond.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... that a change in amount is appropriate to cover the costs and obligations of complying with the... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false Amount of bond. 3904.15 Section 3904.15 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND...

  11. 43 CFR 3904.15 - Amount of bond.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... that a change in amount is appropriate to cover the costs and obligations of complying with the... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false Amount of bond. 3904.15 Section 3904.15 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND...

  12. 20 CFR 625.6 - Weekly amount; jurisdictions; reductions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 3 2012-04-01 2012-04-01 false Weekly amount; jurisdictions; reductions. 625.6 Section 625.6 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR DISASTER UNEMPLOYMENT ASSISTANCE § 625.6 Weekly amount; jurisdictions; reductions. (a) In all...

  13. 42 CFR 1003.104 - Amount of assessment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 5 2014-10-01 2014-10-01 false Amount of assessment. 1003.104 Section 1003.104 Public Health OFFICE OF INSPECTOR GENERAL-HEALTH CARE, DEPARTMENT OF HEALTH AND HUMAN SERVICES OIG AUTHORITIES CIVIL MONEY PENALTIES, ASSESSMENTS AND EXCLUSIONS § 1003.104 Amount of assessment. (a) The OIG...

  14. 42 CFR 1003.104 - Amount of assessment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Amount of assessment. 1003.104 Section 1003.104 Public Health OFFICE OF INSPECTOR GENERAL-HEALTH CARE, DEPARTMENT OF HEALTH AND HUMAN SERVICES OIG AUTHORITIES CIVIL MONEY PENALTIES, ASSESSMENTS AND EXCLUSIONS § 1003.104 Amount of assessment. (a) The OIG...

  15. 42 CFR 1003.104 - Amount of assessment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 5 2013-10-01 2013-10-01 false Amount of assessment. 1003.104 Section 1003.104 Public Health OFFICE OF INSPECTOR GENERAL-HEALTH CARE, DEPARTMENT OF HEALTH AND HUMAN SERVICES OIG AUTHORITIES CIVIL MONEY PENALTIES, ASSESSMENTS AND EXCLUSIONS § 1003.104 Amount of assessment. (a) The OIG...

  16. 40 CFR 35.930-2 - Grant amount.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Grant amount. 35.930-2 Section 35.930-2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works-Clean Water Act § 35.930-2 Grant amount. The...

  17. 29 CFR 531.38 - Amounts deducted for taxes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Amounts deducted for taxes. 531.38 Section 531.38 Labor... Employees § 531.38 Amounts deducted for taxes. Taxes which are assessed against the employee and which are... unemployment insurance taxes, as well as other Federal, State, or local taxes, levies, and assessments....

  18. 50 CFR 296.8 - Amount of award.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Amount of award. 296.8 Section 296.8 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE CONTINENTAL SHELF FISHERMEN'S CONTINGENCY FUND § 296.8 Amount of award. (a)...

  19. 50 CFR 296.8 - Amount of award.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Amount of award. 296.8 Section 296.8 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE CONTINENTAL SHELF FISHERMEN'S CONTINGENCY FUND § 296.8 Amount of award. (a)...

  20. 26 CFR 1.21-2 - Limitations on amount creditable.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 1 2014-04-01 2013-04-01 true Limitations on amount creditable. 1.21-2 Section 1.21-2 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY INCOME TAX INCOME TAXES Changes in Rates During A Taxable Year § 1.21-2 Limitations on amount creditable. (a) Annual...

  1. 26 CFR 1.21-2 - Limitations on amount creditable.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 1 2011-04-01 2009-04-01 true Limitations on amount creditable. 1.21-2 Section 1.21-2 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY INCOME TAX INCOME TAXES Changes in Rates During A Taxable Year § 1.21-2 Limitations on amount creditable. (a) Annual...

  2. 26 CFR 1.21-2 - Limitations on amount creditable.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 1 2010-04-01 2010-04-01 true Limitations on amount creditable. 1.21-2 Section 1.21-2 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY INCOME TAX INCOME TAXES Changes in Rates During A Taxable Year § 1.21-2 Limitations on amount creditable. (a) Annual...

  3. 42 CFR 130.3 - Amount of payments.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Amount of payments. 130.3 Section 130.3 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES COMPASSIONATE PAYMENTS RICKY RAY HEMOPHILIA RELIEF FUND PROGRAM General Provisions § 130.3 Amount of payments. If there are sufficient...

  4. 42 CFR 130.3 - Amount of payments.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Amount of payments. 130.3 Section 130.3 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES COMPASSIONATE PAYMENTS RICKY RAY HEMOPHILIA RELIEF FUND PROGRAM General Provisions § 130.3 Amount of payments. If there are sufficient...

  5. 42 CFR 130.3 - Amount of payments.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false Amount of payments. 130.3 Section 130.3 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES COMPASSIONATE PAYMENTS RICKY RAY HEMOPHILIA RELIEF FUND PROGRAM General Provisions § 130.3 Amount of payments. If there are sufficient...

  6. 42 CFR 130.3 - Amount of payments.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false Amount of payments. 130.3 Section 130.3 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES COMPASSIONATE PAYMENTS RICKY RAY HEMOPHILIA RELIEF FUND PROGRAM General Provisions § 130.3 Amount of payments. If there are sufficient...

  7. 42 CFR 130.3 - Amount of payments.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 1 2013-10-01 2013-10-01 false Amount of payments. 130.3 Section 130.3 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES COMPASSIONATE PAYMENTS RICKY RAY HEMOPHILIA RELIEF FUND PROGRAM General Provisions § 130.3 Amount of payments. If there are sufficient...

  8. 40 CFR 47.115 - Award amount and matching requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Award amount and matching requirements. 47.115 Section 47.115 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE NATIONAL ENVIRONMENTAL EDUCATION ACT GRANTS § 47.115 Award amount and...

  9. 40 CFR 47.115 - Award amount and matching requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Award amount and matching requirements. 47.115 Section 47.115 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE NATIONAL ENVIRONMENTAL EDUCATION ACT GRANTS § 47.115 Award amount and...

  10. 24 CFR 904.108 - Break-even amount.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Break-even amount. 904.108 Section... LOW RENT HOUSING HOMEOWNERSHIP OPPORTUNITIES Turnkey III Program Description § 904.108 Break-even amount. (a) Definition. The term “break-even amount” as used herein means the minimum average...

  11. 40 CFR 35.930-2 - Grant amount.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Grant amount. 35.930-2 Section 35.930-2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works-Clean Water Act § 35.930-2 Grant amount. The...

  12. 40 CFR 35.930-2 - Grant amount.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Grant amount. 35.930-2 Section 35.930-2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works-Clean Water Act § 35.930-2 Grant amount. The...

  13. 40 CFR 35.930-2 - Grant amount.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Grant amount. 35.930-2 Section 35.930-2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works-Clean Water Act § 35.930-2 Grant amount. The...

  14. 40 CFR 35.930-2 - Grant amount.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Grant amount. 35.930-2 Section 35.930-2 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER FEDERAL ASSISTANCE STATE AND LOCAL ASSISTANCE Grants for Construction of Treatment Works-Clean Water Act § 35.930-2 Grant amount. The...

  15. 78 FR 14179 - Adjustments to Civil Monetary Penalty Amounts

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-05

    ... Monetary Penalty Amounts, Release No. 33-8530 (Feb. 4, 2005) [70 FR 7606 (Feb. 14, 2005)]. The DCIA... Commission. ACTION: Final rule. SUMMARY: This rule implements the Federal Civil Penalties Inflation... a rule adjusting for inflation the maximum amount of civil monetary penalties under the...

  16. 20 CFR 404.260 - Special minimum primary insurance amounts.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Special minimum primary insurance amounts. 404.260 Section 404.260 Employees' Benefits SOCIAL SECURITY ADMINISTRATION FEDERAL OLD-AGE, SURVIVORS AND DISABILITY INSURANCE (1950- ) Computing Primary Insurance Amounts Special Minimum...

  17. 41 CFR 105-57.008 - Amounts withheld.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... amount equivalent to thirty times the minimum wage. See 29 CFR 870.10. (c) When a debtor's pay is subject... 41 Public Contracts and Property Management 3 2011-01-01 2011-01-01 false Amounts withheld. 105-57.008 Section 105-57.008 Public Contracts and Property Management Federal Property...

  18. 41 CFR 105-57.008 - Amounts withheld.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... amount equivalent to thirty times the minimum wage. See 29 CFR 870.10. (c) When a debtor's pay is subject... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Amounts withheld. 105-57.008 Section 105-57.008 Public Contracts and Property Management Federal Property...

  19. 20 CFR 226.73 - Changes in reduction amount.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Benefits Under a Federal, State, or Local Law or Plan § 226.73 Changes in reduction amount. The reduction... in annuity rates, such as a cost-of-living increase. However, the reduction amount may change for the... entitled to an annuity with a tier I component of $500 on May 1, 1991. He was also receiving a...

  20. 20 CFR 226.73 - Changes in reduction amount.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Benefits Under a Federal, State, or Local Law or Plan § 226.73 Changes in reduction amount. The reduction... in annuity rates, such as a cost-of-living increase. However, the reduction amount may change for the... entitled to an annuity with a tier I component of $500 on May 1, 1991. He was also receiving a...

  1. 31 CFR 353.10 - Amounts which may be purchased.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... of purchase was an exempt organization under the terms of 26 CFR 1.501(c)(3)-1. ...) Special limitation. $4,000 (face amount) multiplied by the highest number of employees participating in an... issued. (b) Series HH—(1) General annual limitation. $20,000 (face amount). (2) Special limitation....

  2. 31 CFR 353.10 - Amounts which may be purchased.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... of purchase was an exempt organization under the terms of 26 CFR 1.501(c)(3)-1. ...) Special limitation. $4,000 (face amount) multiplied by the highest number of employees participating in an... issued. (b) Series HH—(1) General annual limitation. $20,000 (face amount). (2) Special limitation....

  3. 26 CFR 301.6334-3 - Determination of exempt amount.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... deductions on account of age or blindness); and (ii) The aggregate amount of the deductions for personal... account of age or blindness); and (B) The aggregate amount of the deductions for personal exemptions... standard deduction (including additional standard deductions on account of age or blindness) for...

  4. 26 CFR 1.666(a)-1 - Amount allocated.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 8 2013-04-01 2013-04-01 false Amount allocated. 1.666(a)-1 Section 1.666(a)-1 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED... Beginning Before January 1, 1969 § 1.666(a)-1 Amount allocated. (a)(1) If a trust other than a foreign...

  5. Learner Preferences and Achievement Under Differing Amounts of Learner Practice.

    ERIC Educational Resources Information Center

    Schnackenberg, Heidi L.; Sullivan, Howard J.; Leader, Lars F.; Jones, Elizabeth E. K.

    1998-01-01

    Examines the effects of program mode (i.e., a lean program version containing a basic amount of learner practice versus a full mode containing expanded practice) and learner preference (matched or unmatched) for amount of practice on the achievement, time-in-program, and attitudes of university undergraduate students. Students preferred the lean…

  6. 13 CFR 120.348 - Amount of guarantee.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Amount of guarantee. 120.348 Section 120.348 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Special Purpose Loans International Trade Loans § 120.348 Amount of guarantee. SBA can guarantee up to...

  7. 14 CFR 383.2 - Amount of penalty.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 4 2013-01-01 2013-01-01 false Amount of penalty. 383.2 Section 383.2 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION PROCEEDINGS) SPECIAL REGULATIONS CIVIL PENALTIES § 383.2 Amount of penalty. Civil penalties payable to the U.S. Government...

  8. 42 CFR 438.704 - Amounts of civil money penalties.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Amounts of civil money penalties. 438.704 Section... SERVICES (CONTINUED) MEDICAL ASSISTANCE PROGRAMS MANAGED CARE Sanctions § 438.704 Amounts of civil money penalties. (a) General rule. The limit on, or the maximum civil money penalty the State may impose...

  9. 29 CFR 530.302 - Amounts of civil money penalties.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Amounts of civil money penalties. 530.302 Section 530.302... EMPLOYMENT OF HOMEWORKERS IN CERTAIN INDUSTRIES Civil Money Penalties § 530.302 Amounts of civil money penalties. (a) A civil money penalty, not to exceed $500 per affected homeworker for any one violation,...

  10. 20 CFR 404.342 - Mother's and father's benefit amounts.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Mother's and father's benefit amounts. 404... Disability Benefits for Spouses and Divorced Spouses § 404.342 Mother's and father's benefit amounts. Your mother's or father's monthly benefit is equal to 75 percent of the insured person's primary...

  11. 20 CFR 404.342 - Mother's and father's benefit amounts.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false Mother's and father's benefit amounts. 404... Disability Benefits for Spouses and Divorced Spouses § 404.342 Mother's and father's benefit amounts. Your mother's or father's monthly benefit is equal to 75 percent of the insured person's primary...

  12. 20 CFR 404.353 - Child's benefit amounts.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false Child's benefit amounts. 404.353 Section 404... Child's Benefits § 404.353 Child's benefit amounts. (a) General. Your child's monthly benefit is equal... as explained in § 404.304. (b) Entitlement to more than one benefit. If you are entitled to a...

  13. 20 CFR 404.353 - Child's benefit amounts.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false Child's benefit amounts. 404.353 Section 404... Child's Benefits § 404.353 Child's benefit amounts. (a) General. Your child's monthly benefit is equal... as explained in § 404.304. (b) Entitlement to more than one benefit. If you are entitled to a...

  14. 20 CFR 404.353 - Child's benefit amounts.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Child's benefit amounts. 404.353 Section 404... Child's Benefits § 404.353 Child's benefit amounts. (a) General. Your child's monthly benefit is equal... as explained in § 404.304. (b) Entitlement to more than one benefit. If you are entitled to a...

  15. 20 CFR 404.353 - Child's benefit amounts.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Child's benefit amounts. 404.353 Section 404... Child's Benefits § 404.353 Child's benefit amounts. (a) General. Your child's monthly benefit is equal... as explained in § 404.304. (b) Entitlement to more than one benefit. If you are entitled to a...

  16. 20 CFR 404.353 - Child's benefit amounts.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 2 2013-04-01 2013-04-01 false Child's benefit amounts. 404.353 Section 404... Child's Benefits § 404.353 Child's benefit amounts. (a) General. Your child's monthly benefit is equal... as explained in § 404.304. (b) Entitlement to more than one benefit. If you are entitled to a...

  17. 12 CFR 347.120 - Computation of investment amounts.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 5 2014-01-01 2014-01-01 false Computation of investment amounts. 347.120 Section 347.120 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY INTERNATIONAL BANKING § 347.120 Computation of investment amounts. In computing the...

  18. 12 CFR 347.120 - Computation of investment amounts.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Computation of investment amounts. 347.120 Section 347.120 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY INTERNATIONAL BANKING § 347.120 Computation of investment amounts. In computing the...

  19. 12 CFR 347.120 - Computation of investment amounts.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 5 2012-01-01 2012-01-01 false Computation of investment amounts. 347.120 Section 347.120 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY INTERNATIONAL BANKING § 347.120 Computation of investment amounts. In computing the...

  20. 12 CFR 347.120 - Computation of investment amounts.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 5 2013-01-01 2013-01-01 false Computation of investment amounts. 347.120 Section 347.120 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY INTERNATIONAL BANKING § 347.120 Computation of investment amounts. In computing the...

  1. 12 CFR 347.120 - Computation of investment amounts.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 4 2011-01-01 2011-01-01 false Computation of investment amounts. 347.120 Section 347.120 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY INTERNATIONAL BANKING § 347.120 Computation of investment amounts. In computing the...

  2. The Amount of English Use: Effects on L2 Speech

    ERIC Educational Resources Information Center

    Vo, Son Ca; Vo, Yen Thi Hoang; Vo, Quyen Thanh

    2014-01-01

    The amount of second language (L2) use has significant influence on native speakers' comprehension of L2 learners' speech. Nonetheless, few empirical studies examine how differences in the amount of language use affect the intelligibility and comprehensibility of nonnative speakers' reading and spontaneous speech. This study aims to…

  3. 26 CFR 1.1441-2 - Amounts subject to withholding.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... TAX (CONTINUED) INCOME TAXES (CONTINUED) Withholding of Tax on Nonresident Aliens and Foreign Corporations and Tax-Free Covenant Bonds § 1.1441-2 Amounts subject to withholding. (a) In general. For... certain gambling winnings exempt from tax under section 871(j)); (5) Amounts paid as part of the...

  4. 42 CFR 422.382 - Minimum net worth amount.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... percent of the minimum net worth amount is not met by cash or cash equivalents. (3) Health care delivery... depreciated value of health care delivery assets (HCDAs) to satisfy the minimum net worth amount. (4) Other assets. A PSO may apply other assets not used in the delivery of health care provided that those...

  5. 46 CFR 308.303 - Amounts insured under interim binder.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 8 2011-10-01 2011-10-01 false Amounts insured under interim binder. 308.303 Section 308.303 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE Second Seamen's War Risk Insurance § 308.303 Amounts insured under interim binder. The...

  6. 46 CFR 308.203 - Amount insured under interim binder.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 8 2011-10-01 2011-10-01 false Amount insured under interim binder. 308.203 Section 308.203 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION EMERGENCY OPERATIONS WAR RISK INSURANCE War Risk Protection and Indemnity Insurance § 308.203 Amount insured under interim binder....

  7. 42 CFR 423.279 - National average monthly bid amount.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... bid amounts for each prescription drug plan (not including fallbacks) and for each MA-PD plan...(h) of the Act. (b) Calculation of weighted average. (1) The national average monthly bid amount is a....258(c)(1) of this chapter) and the denominator equal to the total number of Part D...

  8. 42 CFR 423.279 - National average monthly bid amount.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... bid amounts for each prescription drug plan (not including fallbacks) and for each MA-PD plan...(h) of the Act. (b) Calculation of weighted average. (1) The national average monthly bid amount is a....258(c)(1) of this chapter) and the denominator equal to the total number of Part D...

  9. 14 CFR § 1261.102 - Maximum amount.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 5 2014-01-01 2014-01-01 false Maximum amount. § 1261.102 Section § 1261.102 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION PROCESSING OF MONETARY CLAIMS (GENERAL) Employees' Personal Property Claims § 1261.102 Maximum amount. From October 1, 1982, to...

  10. 9 CFR 89.1 - Amount of feed.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Amount of feed. 89.1 Section 89.1 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS STATEMENT OF POLICY UNDER THE TWENTY-EIGHT HOUR LAW § 89.1 Amount of feed....

  11. 45 CFR 305.31 - Amount of incentive payment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Amount of incentive payment. 305.31 Section 305.31... PROGRAM PERFORMANCE MEASURES, STANDARDS, FINANCIAL INCENTIVES, AND PENALTIES § 305.31 Amount of incentive payment. (a) The incentive payment for a State for a fiscal year is equal to the incentive payment...

  12. 20 CFR 226.73 - Changes in reduction amount.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Benefits Under a Federal, State, or Local Law or Plan § 226.73 Changes in reduction amount. The reduction... in annuity rates, such as a cost-of-living increase. However, the reduction amount may change for the... entitled to an annuity with a tier I component of $500 on May 1, 1991. He was also receiving a...

  13. 20 CFR 226.73 - Changes in reduction amount.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Benefits Under a Federal, State, or Local Law or Plan § 226.73 Changes in reduction amount. The reduction... in annuity rates, such as a cost-of-living increase. However, the reduction amount may change for the... entitled to an annuity with a tier I component of $500 on May 1, 1991. He was also receiving a...

  14. 34 CFR 682.204 - Maximum loan amounts.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... academic year in 34 CFR 668.3. (m) Any TEACH Grants that have been converted to Direct Unsubsidized Loans... amounts under paragraph (c)(1)(ii) of this section. (e) Combined Federal Stafford, SLS and Federal... Loans and SLS Loans, but excluding the amount of capitalized interest, may not exceed the following:...

  15. 34 CFR 682.204 - Maximum loan amounts.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... academic year in 34 CFR 668.3. (m) Any TEACH Grants that have been converted to Direct Unsubsidized Loans... amounts under paragraph (c)(1)(ii) of this section. (e) Combined Federal Stafford, SLS and Federal... Loans and SLS Loans, but excluding the amount of capitalized interest, may not exceed the following:...

  16. 34 CFR 682.204 - Maximum loan amounts.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... academic year in 34 CFR 668.3. (m) Any TEACH Grants that have been converted to Direct Unsubsidized Loans... amounts under paragraph (c)(1)(ii) of this section. (e) Combined Federal Stafford, SLS and Federal... Loans and SLS Loans, but excluding the amount of capitalized interest, may not exceed the following:...

  17. 26 CFR 1.615-4 - Limitation of amount deductible.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 7 2011-04-01 2009-04-01 true Limitation of amount deductible. 1.615-4 Section 1.615-4 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Natural Resources § 1.615-4 Limitation of amount deductible. (a) Taxable years beginning before July 7,...

  18. 26 CFR 1.617-2 - Limitation on amount deductible.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 7 2011-04-01 2009-04-01 true Limitation on amount deductible. 1.617-2 Section 1.617-2 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Natural Resources § 1.617-2 Limitation on amount deductible. (a) Expenditures paid or incurred before January...

  19. 26 CFR 1.615-4 - Limitation of amount deductible.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Limitation of amount deductible. 1.615-4 Section 1.615-4 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Natural Resources § 1.615-4 Limitation of amount deductible. (a)...

  20. 26 CFR 1.617-2 - Limitation on amount deductible.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Limitation on amount deductible. 1.617-2 Section 1.617-2 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES (CONTINUED) Natural Resources § 1.617-2 Limitation on amount deductible....