Science.gov

Sample records for accumulation rates derived

  1. Net accumulation rates derived from ice core stable isotope records of Pío XI glacier, Southern Patagonia Icefield

    NASA Astrophysics Data System (ADS)

    Schwikowski, M.; Schläppi, M.; Santibañez, P.; Rivera, A.; Casassa, G.

    2012-12-01

    Pío XI, the largest glacier of the Southern Patagonia Icefield, reached its neoglacial maximum extent in 1994 and is one of the few glaciers in that area which is not retreating. In view of the recent warming it is important to understand glacier responses to climate changes. Due to its remoteness and the harsh conditions in Patagonia, no systematic mass balance studies have been performed. In this study we derived net accumulation rates for the period 2000 to 2006 from a 50 m (33.2 4 m weq) ice core collected in the accumulation area of Pío XI (2600 m a.s.l., 49°16´40´´ S, 73°21´14´´ W). Borehole temperatures indicate near temperate ice, but the average melt percent is only 16% ± 14%. Records of stable isotopes are well preserved and were used for identification of annual layers. Net accumulation rates range from 3.4 to 7.1 water equivalent (m weq) with an average of 5.8 m weq, comparable to precipitation amounts at the Chilean coast, but not as high as expected for the Icefield. Ice core stable isotope data correlate well with upper air temperatures and may be used as temperature proxy.

  2. Comparison of annual accumulation rates derived from in situ and ground penetrating radar methods across Alaskan glaciers

    NASA Astrophysics Data System (ADS)

    McGrath, D.; Gusmeroli, A.; Oneel, S.; Sass, L. C.; Arendt, A. A.; Wolken, G. J.; Kienholz, C.; McNeil, C.

    2013-12-01

    Constraining annual snowfall accumulation in mountain glacier environments is essential for determining the annual mass balance of individual glaciers and predicting seasonal meltwater runoff to river and marine ecosystems. However, large spatial and elevation gradients, coupled with sparse point measurements preclude accurate quantification of this variable using traditional methods. Here, we report on an extensive field campaign conducted in March-May 2013 on key benchmark glaciers in Alaska, including Taku Glacier near Juneau, Scott Glacier near Cordova, both Eklutna and Wolverine Glacier near Anchorage and Gulkana Glacier in the interior Alaska Range. Over 50 km of 500 MHz common-offset ground penetrating radar (GPR) surveys were collected on each glacier, with an emphasis on capturing spatial variability in the accumulation zone. Frequent in situ observations were collected for comparison with the GPR, including probe depths, snow pits and shallow firn cores (~8 m). We report on spatial and elevation gradients across this suite of glaciers and across numerous climatic zones and discuss differences between GPR and in situ derived annual accumulation estimates. This comparison is an essential first step in order to effectively evaluate regional atmospheric re-analysis products.

  3. Biogeochemical cycling in an organic-rich coastal marine basin. 9. Sources and accumulation rates of vascular plant-derived organic material

    SciTech Connect

    Haddad, R.I.; Martens, C.S. )

    1987-11-01

    The sources, degradation and burial of vascular plant debris deposited over the past several decades in the lagoonal sediments of Cape Lookout Bight, North Carolina, are quantified using alkaline cupric oxide lignin oxidation product (LOP) analysis. Non-woody angiosperms, accounting for 92 {plus minus} 32% of the recognizable sedimentary vascular plant debris, are calculated to contribute 23 {plus minus} 17% of the total organic carbon buried over the past decade. When combined with a previously established sedimentary organic carbon budget for this site a vascular plant derived carbon burial rate of 26 {plus minus}20 mole C m{sup {minus}2} yr{sup {minus}1} is calculated for this same time interval. The refractory nature and invariant depth distributions of the lignin oxidation products (LOP), when coupled with evidence for constant degradation rates of metabolizable materials, indicate that sediment accumulation at this site has been a steady state process with respect to source and burial of organic carbon since its conversion from an inner-continental shelf to a lagoonal environment during the late 1960's. Thus systematic down-core decreases in labile organic matter result from early diagenetic processes rather than input rate variations.

  4. Variation of Accumulation Rates Over the Last Eight Centuries on the East Antarctic Plateau Derived from Volcanic Signals in Ice Cores

    NASA Technical Reports Server (NTRS)

    Anschuetz, H.; Sinisalo, A.; Isaksson, E.; McConnell, J. R.; Hamran, S.-E.; Bisiaux, M. M.; Pasteris, D.; Neumann, T. A.; Winther, J.-G.

    2011-01-01

    Volcanic signatures in ice-core records provide an excellent means to date the cores and obtain information about accumulation rates. From several ice cores it is thus possible to extract a spatio-temporal accumulation pattern. We show records of electrical conductivity and sulfur from firn cores from the Norwegian-USA scientific traverse during the International Polar Year 2007-2009 (IPY) through East Antarctica. Major volcanic eruptions are identified and used to assess century-scale accumulation changes. The largest changes seem to occur in the most recent decades with accumulation over the period 1963- 2007/08 being up to 25 % different from the long-term record. There is no clear overall trend, some sites show an increase in accumulation over the period 1963 to present while others show a decrease. Almost all of the sites above 3200 m above sea level (asl) suggest a decrease. These sites also show a significantly lower accumulation value than large-scale assessments both for the period 1963 to present and for the long-term mean at the respective drill sites. The spatial accumulation distribution is influenced mainly by elevation and distance to the ocean (continentality), as expected. Ground-penetrating radar data around the drill sites show a spatial variability within 10-20 % over several tens of kilometers, indicating that our drill sites are well representative for the area around them. Our results are important for large-scale assessments of Antarctic mass balance and model validation.

  5. Determining Greenland Ice Sheet Accumulation Rates from Radar Remote Sensing

    NASA Technical Reports Server (NTRS)

    Jezek, Kenneth C.

    2002-01-01

    An important component of NASA's Program for Arctic Regional Climate Assessment (PARCA) is a mass balance investigation of the Greenland Ice Sheet. The mass balance is calculated by taking the difference between the areally Integrated snow accumulation and the net ice discharge of the ice sheet. Uncertainties in this calculation Include the snow accumulation rate, which has traditionally been determined by interpolating data from ice core samples taken from isolated spots across the ice sheet. The sparse data associated with ice cores juxtaposed against the high spatial and temporal resolution provided by remote sensing , has motivated scientists to investigate relationships between accumulation rate and microwave observations as an option for obtaining spatially contiguous estimates. The objective of this PARCA continuation proposal was to complete an estimate of surface accumulation rate on the Greenland Ice Sheet derived from C-band radar backscatter data compiled in the ERS-1 SAR mosaic of data acquired during, September-November, 1992. An empirical equation, based on elevation and latitude, is used to determine the mean annual temperature. We examine the influence of accumulation rate, and mean annual temperature on C-band radar backscatter using a forward model, which incorporates snow metamorphosis and radar backscatter components. Our model is run over a range of accumulation and temperature conditions. Based on the model results, we generate a look-up table, which uniquely maps the measured radar backscatter, and mean annual temperature to accumulation rate. Our results compare favorably with in situ accumulation rate measurements falling within our study area.

  6. Mass accumulation rates in Asia during the Cenozoic

    NASA Astrophysics Data System (ADS)

    Métivier, François; Gaudemer, Yves; Tapponnier, Paul; Klein, Michel

    1999-05-01

    This work establishes estimates of mass accumulation rates in 18 mostly offshore sedimentary basins in Asia since the beginning of the Cenozoic, ~ 66 Ma. The estimates were derived from isopach maps, cross-sections and drill holes or stratigraphic columns assuming regional similarity of the strata. Average solid phase volumes and accumulation rates were calculated for nine epochs approximately corresponding to geological periods: Palaeocene ( ~ 66-58 Ma), Eocene ( ~ 58-37 Ma), Oligocene( ~ 37-30 and 30-24 Ma), Miocene ( ~ 24-17, 17-11 and 11-5 Ma), Pliocene ( ~ 5-2 Ma) and Quaternary ( ~ 2-0 Ma). These rates shed new light on the geological history of Asia since the onset of the collision of India with Asia ( ~ 50 Ma). The overall average accumulation rates curve for Asian sedimentary basins since the beginning of the Tertiary shows an exponential form with slow accumulation rates (less than 0.5 x 10^6 km^3 Myr^- 1) until the beginning of the Oligocene, more than 15 Myr after the onset of the collision. From the Oligocene onwards rates increase quickly in an exponential manner, reaching their maximum values in the Quaternary (more than 1.5 x 10^6 km^3 Myr^- 1). From these observations we suggest that extrusion and crustal shortening are complementary processes that have been successively dominant throughout the India-Eurasia collision history. At smaller scales one may distinguish between independent histories at the subcontinental and basin scales. This permits a comparison of the relative importance of tectonic and climatic erosion processes affecting the different mountain belts of Asia during the Cenozoic.

  7. Rates and controls on N accumulation in peatlands

    NASA Astrophysics Data System (ADS)

    Zivkovic, T.; Wang, M.; Moore, T. R.; Loisel, J.

    2013-12-01

    Paleoecological studies on peat cores have focused mainly on carbon (C) accumulation rates, whereas nitrogen (N) accumulation rates and cycling have been largely overlooked. We use primary data from peat cores extracted from Mer Bleue bog, the Northwest Territories and eastern and western Canada to estimate long- and short-term N accumulation rates. Furthermore, we apply the mean C/N ratios from a wide range of peatland types in Ontario to estimate N accumulation rates where C accumulation rates are available. Rates of N accumulation range from 0.1 to 2.0 g m-2 yr-1. We examine the sources of N to peatlands and different peatland types (bogs, fens and swamps) depend on N from different sources. For example, ombrotrophic bogs depend on bulk atmospheric N deposition and biological N2 fixation as their only source of N. Oligo- and minerotrophic fens however receive additional N along with other nutrients from the surface and ground water. Prior to Industrial Revolution atmospheric N deposition in peatlands was minimal and likely constant (< 0.1 g m-2 yr-1). Although it is impossible to measure N2 fixation rates in the past, N accumulation rates represent an overall balance between N inputs and outputs in these ecosystems. In bogs, N outputs are small, thus N accumulation rates could be explained by N2 fixation rates that have been the main source of N for these ecosystems, and we compare N accumulation rates with current measurements of N2 fixation.

  8. Annual Greenland accumulation rates (2009-2012) from airborne snow radar

    NASA Astrophysics Data System (ADS)

    Koenig, Lora S.; Ivanoff, Alvaro; Alexander, Patrick M.; MacGregor, Joseph A.; Fettweis, Xavier; Panzer, Ben; Paden, John D.; Forster, Richard R.; Das, Indrani; McConnell, Joesph R.; Tedesco, Marco; Leuschen, Carl; Gogineni, Prasad

    2016-08-01

    Contemporary climate warming over the Arctic is accelerating mass loss from the Greenland Ice Sheet through increasing surface melt, emphasizing the need to closely monitor its surface mass balance in order to improve sea-level rise predictions. Snow accumulation is the largest component of the ice sheet's surface mass balance, but in situ observations thereof are inherently sparse and models are difficult to evaluate at large scales. Here, we quantify recent Greenland accumulation rates using ultra-wideband (2-6.5 GHz) airborne snow radar data collected as part of NASA's Operation IceBridge between 2009 and 2012. We use a semiautomated method to trace the observed radiostratigraphy and then derive annual net accumulation rates for 2009-2012. The uncertainty in these radar-derived accumulation rates is on average 14 %. A comparison of the radar-derived accumulation rates and contemporaneous ice cores shows that snow radar captures both the annual and long-term mean accumulation rate accurately. A comparison with outputs from a regional climate model (MAR) shows that this model matches radar-derived accumulation rates in the ice sheet interior but produces higher values over southeastern Greenland. Our results demonstrate that snow radar can efficiently and accurately map patterns of snow accumulation across an ice sheet and that it is valuable for evaluating the accuracy of surface mass balance models.

  9. Annual Greenland Accumulation Rates (2009-2012) from Airborne Snow Radar

    NASA Technical Reports Server (NTRS)

    Koenig, Lora S.; Ivanoff, Alvaro; Alexander, Patrick M.; MacGregor, Joseph A.; Fettweis, Xavier; Panzer, Ben; Paden, John D.; Forster, Richard R.; Das, Indrani; McConnell, Joseph R.; Tedesco, Marco; Leuschen, Carl; Gogineni, Prasad

    2016-01-01

    Contemporary climate warming over the Arctic is accelerating mass loss from the Greenland Ice Sheet through increasing surface melt, emphasizing the need to closely monitor its surface mass balance in order to improve sea-level rise predictions. Snow accumulation is the largest component of the ice sheet's surface mass balance, but in situ observations thereof are inherently sparse and models are difficult to evaluate at large scales. Here, we quantify recent Greenland accumulation rates using ultra-wideband (2-6.5 gigahertz) airborne snow radar data collected as part of NASA's Operation IceBridge between 2009 and 2012. We use a semi-automated method to trace the observed radiostratigraphy and then derive annual net accumulation rates for 2009-2012. The uncertainty in these radar-derived accumulation rates is on average 14 percent. A comparison of the radarderived accumulation rates and contemporaneous ice cores shows that snow radar captures both the annual and longterm mean accumulation rate accurately. A comparison with outputs from a regional climate model (MAR - Modele Atmospherique Regional for Greenland and vicinity) shows that this model matches radar-derived accumulation rates in the ice sheet interior but produces higher values over southeastern Greenland. Our results demonstrate that snow radar can efficiently and accurately map patterns of snow accumulation across an ice sheet and that it is valuable for evaluating the accuracy of surface mass balance models.

  10. Determining Greenland Ice Sheet Accumulation Rates from Radar Remote Sensing

    NASA Technical Reports Server (NTRS)

    Jezek, Kenneth C.

    2001-01-01

    An important component of NASA's Program for Arctic Regional Climate Assessment (PARCA) is a mass balance investigation of the Greenland Ice Sheet. The mass balance is calculated by taking the difference between the snow accumulation and the ice discharge of the ice sheet. Uncertainties in this calculation include the snow accumulation rate, which has traditionally been determined by interpolating data from ice core samples taken throughout the ice sheet. The sparse data associated with ice cores, coupled with the high spatial and temporal resolution provided by remote sensing, have motivated scientists to investigate relationships between accumulation rate and microwave observations.

  11. Greenland Ice Sheet Annually-resolved Accumulation Rates (1958-2007), a Spatially Calibrated Model

    NASA Astrophysics Data System (ADS)

    Burgess, E. W.; Forster, R. R.; Box, J. W.; Smith, L. C.; Bromwich, D. H.

    2008-12-01

    The Greenland Ice Sheet (GIS) has responded dramatically to recent temperature increases, making it an important contributor to sea level rise. Accurate predictions of Greenland's future contribution to sea level will require a scrupulous understanding of the GIS system and refining our understanding of accumulation is a critical step towards this goal. The most accurate existing estimates of Greenland accumulation rates are multi-year averages; existing annual estimates contain poorly quantified uncertainties. This project developed a superior Greenland accumulation dataset that is spatially comprehensive, has annual resolution, is calibrated to field observations and contains sound uncertainty estimates. Accumulation output from a 1958- 2007 run of the Fifth Generation Mesoscale Model modified for polar climates (PMM5) was calibrated against 133 firn cores and coastal meteorological stations. PMM5 accumulation rate estimates contained spatially dependent systematic biases that were modeled and removed using spatial interpolation of zonally derived regressions. The calibrated accumulation dataset contains residual uncertainties exhibiting a strong spatial pattern that was modeled to estimate ice-sheet wide uncertainty. No significant 1958-2007 trends in Greenland accumulation are evident. Average annual accumulation rate is estimated at 0.339m.w.e. or 593km3 with an RMSE uncertainty of +/-83 km3 or +/-14%. The accumulation dataset will be made publicly available.

  12. Holocene and recent sediment accumulation rates in southern Lake Michigan

    USGS Publications Warehouse

    Colman, Steven M.; King, J.W.; Jones, Glenn A.; Reynolds, R.L.; Bothner, Michael H.

    2000-01-01

    Rates of sediment accumulation in Lake Michigan are a key component of its geologic history and provide important data related to societal concerns such as shoreline erosion and the fate of anthropogenic pollutants. Previous attempts to reconstruct Holocene rates of sediment accumulation in Lake Michigan, as well as in the other Laurentian Great Lakes, have been bedeviled by the effect of refractory terrestrial material on radiocarbon ages from total organic carbon samples of lake sediments. AMS radiocarbon ages on small samples of biogenic carbonate (ostracodes and mollusks) in Lake Michigan provide accurate Holocene ages. The present bicarbonate reservoir effect is estimated from shells of mollusks collected live before atmospheric nuclear testing to be 250 yr. From paired samples of biogenic carbonate and terrestrial macrofossils, the past reservoir effect is thought to be less than 500 yr. The radiocarbon ages indicate a distinct decrease in sediment accumulation rates throughout the southern basin of Lake Michigan at about 5 ka, about the time when lake level stabilized at the Nipissing level after rising rapidly for several thousand years. Average rates of sediment accumulation for the historic period (the last 150 yr) can be estimated from radioisotopes (210Pb and 137Cs), pollen stratigraphy, and changes in sediment properties associated with human activity. Multiple methods are necessary because at any given site, problems arise in the assumptions or applicability of one or more methods. In general, the mass accumulation calculations suggest that sediments were deposited 4 to 11 times faster in the historic period than before human settlement. The character of the sediment did not change in a dramatic way, but sediment magnetic properties suggest shifts in the sources of sediment. The data suggest that some of the changes in sources and (or) character of the sediment occurred just before human settlement and were probably related to climatic changes

  13. Accumulation rates of airborne heavy metals in wetlands

    USGS Publications Warehouse

    Souch, C.J.; Filippelli, G.M.; Dollar, N.; Perkins, S.; Mastalerz, Maria

    2002-01-01

    Accumulation rates of heavy metals (Cd, Cr, Cu, Mn, Pb, and Zn) retained in wetland sediments in northwest Indiana-downwind of the Chicago-Gary-Hammond industrial area-are quantified to assess anthropogenic influences on atmospheric fluxes. Metal concentrations for 22 sediment cores are determined by ICP-AES after ashing and strong acid extraction. Relations between organic content and metal concentrations at depth are used to separate natural and anthropogenic sources. Accumulation rates over the lifetime of the wetlands (???4500 years) have averaged 0.2 (Cd), 1.4 (Cu), 1.7 (Cr), 13.4 (Mn), 4.8 (Pb), and 18.7 (Zn) mg m-2 y-1. Rates for the last 100 years have increased on average by factors of 6 (Cd), 8 (Cu), 10 (Mn), 15 (Pb), and 30 (Zn), remaining effectively constant for Cr. Where the wetlands have been drained, metals have been lost from the sediments, owing to changes in organic content and local hydrochemistry (exposure to acidic rainfall). Sediment-based accumulation rates at the undrained sites are higher, though generally consistent, with measured and modeled atmospheric fluxes documented by short-term studies conducted over the last three decades. The fraction of the total metals in the wetlands estimated to be of anthropogenic origin ranges from approximately 3% for Cr, up to approximately 35% for Pb, and 70% for Zn. This historic legacy of contamination must be considered in land management decisions, particularly when wetlands are drained.

  14. Radon exhalation rates from building materials using electret ion chamber radon monitors in accumulators.

    PubMed

    Kotrappa, Payasada; Stieff, Frederick

    2009-08-01

    An electret ion chamber (EIC) radon monitor in a sealed accumulator measures the integrated average radon concentration at the end of the accumulation duration. Theoretical equations have been derived to relate such radon concentrations (Bq m(-3) ) to the radon emanation rate (Bq d(-1)) from building materials enclosed in the accumulator. As an illustration, a 4-L sealable glass jar has been used as an accumulator to calculate the radon emanation rate from different granite samples. The radon emanation rate was converted into radon flux (Bq mm(-2) d(-1)) by dividing the emanation rate by surface area of the sample. Fluxes measured on typical, commercially available granites ranged from 20-30 Bq m(-2) d(-1). These results are similar to the results reported in the literature. The lower limit of detection for a 2-d measurement works out to be 7 Bq m(-2) d(-1). Equations derived can also be used for other sealable accumulators and other integrating detectors, such as alpha track detectors.

  15. Annual Greenland accumulation derived from airborne radar and comparisons to modeled and in situ data

    NASA Astrophysics Data System (ADS)

    Koenig, L.; Ivanoff, A.; Alexander, P. M.; MacGregor, J. A.; Cullather, R. I.; Nowicki, S.

    2015-12-01

    Mass loss across the Greenland Ice Sheet (GrIS) has accelerated in recent decades and recently a fundamental change in the nature of this mass loss has begun. The dominant GrIS mass-loss process has switched from ice dynamics to surface mass balance (SMB) processes, including melt generation and runoff. This recent shift further emphasizes the need to monitor and constrain SMB, which, across most of the GrIS, is dominated by accumulation. High resolution, near-surface radar data have shown good fidelity at mapping spatial patterns of accumulation to validate model outputs. To better constrain accumulation over the GrIS, we derive annual accumulation rates using NASA Operation IceBridge (OIB) Snow Radar data collected from 2009 through 2012. Accumulation is calculated using the radar-determined depth to an annual layer and the local snow/firn density profile. Up to 30 years of annual stratigraphy is observed in the interior of the ice sheet, near Summit Station, while only the past year is detectable in the ablation zone around the perimeter of the ice sheet. Annual layering is traced using a semi-automatic algorithm and mapped across large areas (tens of thousands of line kilometers). A combined measured and modeled density profile is used to convert the annual stratigraphy into accumulation. Modeled density profiles from the Modèle Atmosphérique Régional (MAR) model are shown to be less than half of in situ observations in the top 1 m of snow/firn and are, therefore, replaced with in situ measurements. Using a compilation of in situ measurements, the mean GrIS snow/firn density is found to be ~340 +/- 40 kg/m3 in the top 1 m. Error in the snow density profile represents the largest error in the radar-derived accumulation. The pattern of radar-derived accumulation rate compares well with MAR estimates, although the latter has a mean bias of 4.6 cm water equivalent, a root mean square error of 16.8 cm water equivalent and a correlation coefficient of 0.6 across

  16. Death rates reflect accumulating brain damage in arthropods

    PubMed Central

    Fonseca, Duane B; Brancato, Carolina L; Prior, Andrew E; Shelton, Peter M.J; Sheehy, Matt R.J

    2005-01-01

    We present the results of the first quantitative, whole-lifespan study of the relationship between age-specific neurolipofuscin concentration and natural mortality rate in any organism. In a convenient laboratory animal, the African migratory locust, Locusta migratoria, we find an unusual delayed-onset neurolipofuscin accumulation pattern that is highly correlated with exponentially accelerating age-specific Gompertz–Makeham death rates in both males (r=0.93, p=0.0064) and females (r=0.97, p=0.0052). We then test the conservation of this association by aggregating the locust results with available population-specific data for a range of other terrestrial, freshwater, marine, tropical and temperate arthropods whose longevities span three orders of magnitude. This synthesis shows that the strong association between neurolipofuscin deposition and natural mortality is a phylogenetically and environmentally widespread phenomenon (r=0.96, p<0.0001). These results highlight neurolipofuscin as a unique and outstanding integral biomarker of ageing. They also offer compelling evidence for the proposal that, in vital organs like the brain, either the accumulation of toxic garbage in the form of lipofuscin itself, or the particular molecular reactions underlying lipofuscinogenesis, including free-radical damage, are the primary events in senescence. PMID:16191601

  17. Rate of accumulation of reproductive isolation by chromosome rearrangements

    SciTech Connect

    Walsh, J.B.

    1981-09-01

    The role of chromosome rearrangements as agents for rapid speciation has recently gained considerable support among evolutionary biologists, especially for the stasipatric model in which effective isolation is accomplished by fixation of a few strongly underdominant rearrangements. This support is, however, by no means universal, with critics citing the low probability of fixation of strongly underdominant rearrangements as a major problem of the stasipatric model. Population genetic considerations of the substitution rate of underdominant rearrangements in a finite population were examined by Lande, but as an estimator of long term effective deme size, rather than as a speciation model. The rate of accumulation of postzygotic isolation is analyzed for three models of underdominant rearrangements: strict underdominance; underdominance with the rearrangement homozygote being at a selective advantage; and underdominance with meiotic drive. It will be shown that reproductive isolation accumulates most rapidly in very small populations, but that strong homozygote advantage or drive can allow for fairly rapid speciation under certain conditions in large populations. Contrary to the stasipatric model, isolation usually proceeds most rapidly by fixation of a large number of weakly underdominant rearrangements even when moderate amounts of drive or homozygote advantage are allowed. Stasipatric speciation requires either the frequent occurrence of meiotically driven rearrangements or very small population sizes to operate in reasonable times.

  18. Death rates reflect accumulating brain damage in arthropods.

    PubMed

    Fonseca, Duane B; Brancato, Carolina L; Prior, Andrew E; Shelton, Peter M J; Sheehy, Matt R J

    2005-09-22

    We present the results of the first quantitative, whole-lifespan study of the relationship between age-specific neurolipofuscin concentration and natural mortality rate in any organism. In a convenient laboratory animal, the African migratory locust, Locusta migratoria, we find an unusual delayed-onset neurolipofuscin accumulation pattern that is highly correlated with exponentially accelerating age-specific Gompertz-Makeham death rates in both males (r=0.93, p=0.0064) and females (r=0.97, p=0.0052). We then test the conservation of this association by aggregating the locust results with available population-specific data for a range of other terrestrial, freshwater, marine, tropical and temperate arthropods whose longevities span three orders of magnitude. This synthesis shows that the strong association between neurolipofuscin deposition and natural mortality is a phylogenetically and environmentally widespread phenomenon (r=0.96, p < 0.0001). These results highlight neurolipofuscin as a unique and outstanding integral biomarker of ageing. They also offer compelling evidence for the proposal that, in vital organs like the brain, either the accumulation of toxic garbage in the form of lipofuscin itself, or the particular molecular reactions underlying lipofuscinogenesis, including free-radical damage, are the primary events in senescence.

  19. A flight experiment to determine GPS photochemical contamination accumulation rates

    NASA Technical Reports Server (NTRS)

    Tribble, A. C.; Haffner, J. W.

    1990-01-01

    It was recently suggested that photochemically deposited contamination, originating from volatiles outgassed by a spacecraft, may be responsible for the anomalous degradation in power seen on the GPS Block 1 vehicles. In an attempt to confirm, or deny, the photochemical deposition rates predicted, a study was undertaken to design a flight experiment to be incorporated on the GPS vehicles currently in production. The objective was to develop an inexpensive, light weight instrument package that would give information on the contamination levels within a few months of launch. Three types of apparatus were studied, Quartz Crystal Microbalances, (QCM's), modified solar cells, and calorimeters. A calorimeter was selected due primarily to its impact on the production schedule of the GPS vehicles. An analysis of the sensitivity of the final design is compared to the predicted contamination accumulation rates in order to determine how long after launch it will take the experiment to show the effects of photochemical contamination.

  20. An integral method to estimate the moment accumulation rate on the Creeping Section of the San Andreas Fault

    NASA Astrophysics Data System (ADS)

    Tong, Xiaopeng; Sandwell, David T.; Smith-Konter, Bridget

    2015-10-01

    Moment accumulation rate (also referred to as moment deficit rate) is a fundamental quantity for evaluating seismic hazard. The conventional approach for evaluating moment accumulation rate of creeping faults is to invert for the slip distribution from geodetic measurements, although even with perfect data these slip-rate inversions are non-unique. In this study, we show that the slip-rate versus depth inversion is not needed because moment accumulation rate can be estimated directly from surface geodetic data. We propose an integral approach that uses dense geodetic observations from Interferometric Synthetic Aperture Radar (InSAR) and the Global Positioning System (GPS) to constrain the moment accumulation rate. The moment accumulation rate is related to the integral of the product of the along-strike velocity and the distance from the fault. We demonstrate our methods by studying the Creeping Section of the San Andreas fault observed by GPS and radar interferometry onboard the ERS and ALOS satellites. Along-strike variation of the moment accumulation rate is derived in order to investigate the degree of partial locking of the Creeping Section. The central Creeping Segment has a moment accumulation rate of 0.25-3.1 × 1015 Nm yr-1 km-1. The upper and lower bounds of the moment accumulation rates are derived based on the statistics of the noise. Our best-fitting model indicates that the central portion of the Creeping Section is accumulating seismic moment at rates that are about 5 per cent to 23 per cent of the fully locked Carrizo segment that will eventually be released seismically. A cumulative moment budget calculation with the historical earthquake catalogue (M > 5.5) since 1857 shows that the net moment deficit at present is equivalent to a Mw 6.3 earthquake.

  1. Correlation of thrombosis growth rate to pathological wall shear rate during platelet accumulation.

    PubMed

    Bark, David L; Para, Andrea N; Ku, David N

    2012-10-01

    Local hemodynamics may strongly influence atherothrombosis, which can lead to acute myocardial infarction and stroke. The relationship between hemodynamics and thrombosis during platelet accumulation was studied through an in vitro flow system consisting of a stenosis. Specifically, wall shear rates (WSR) ranging from 0 to 100,000 s(-1) were ascertained through computations and compared with thrombus growth rates found by image analysis for over 5,000 individual observation points per experiment. A positive correlation (P < 0.0001) was found between thrombus accumulation rates and WSR up to 6,000 s(-1), with a decrease in growth rates at WSR >6,000 s(-1) (P < 0.0001). Furthermore, growth rates at pathological shear rates were found to be two to four times greater than for physiological arterial shear rates below 400 s(-1). Platelets did not accumulate for the first minute of perfusion. The initial lag time, before discernible thrombus growth could be found, diminished with shear (P < 0.0001). These studies show the quantitative increase in thrombus growth rates with very high shear rates in stenoses onto a collagen substrate. PMID:22539078

  2. Synthesis of passive microwave and radar altimeter data for estimating accumulation rates of polar snow

    NASA Technical Reports Server (NTRS)

    Davis, Curt H.

    1993-01-01

    In this paper, we compare dry-snow extinction coefficients derived from radar altimeter data with brightness temperature data from passive microwave measurements over a portion of the East Antarctic plateau. The comparison between the extinction coefficients and the brightness temperatures shows a strong negative correlation, where the correlation coefficients ranged from -0.87 to -0.95. The extinction coefficient of the dry polar snow decreases with increasing surface elevation, while the average brightness temperature increases with surface elevation. Our analysis shows that the observed trends are related to geographic variations in scattering coefficient of snow, which in turn are controlled by variations in surface temperature and snow accumulation rate. By combining information present in the extinction coefficient and brightness temperature data sets, we develop a model that can be used to obtain quantitative estimates of the accumulation rate of dry polar snow.

  3. Accumulation rates of biogenous silica and terrigenous detritus in late Miocene-early Pliocene Santa Maria basin, California

    SciTech Connect

    Ramiriz, P.C.

    1988-03-01

    The upper-Miocene-lower Pliocene laminated to massive diatomaceous strata of the Santa Maria basin encompass portions of the Monterey and overlying conformable to nonconformable Sisquoc Formations and record the continued accumulation of admixed siliceous (mostly diatoms) and terrigenous (silt and clay) components in a Neogene borderland basin. Lithologies, which vary from diatomite to diatomaceous mudstone, are the result of fluctuations in slope-accumulating silica and terrigenous debris. Bulk accumulation rates, as well as silica and terrigenous component accumulation rates, were calculated for four measured sections using accurate stratal thicknesses, absolute ages, rock compositions, and bulk densities. Bulk accumulation rates range from less than 20 to greater than 70 mg/cm/sup 2//year and are comparable to bulk accumulation rates in the present-day Santa Barbara basin and Gulf o f California. Silica accumulation rates (20-30 mg/cm/sup 2//year) suggest that sedimentation took place beneath productive waters and they exceed those encountered in other high-productivity oceanic areas, such as the Bering Sea and Peruvian coastal waters. Relatively high terrigenous accumulation rates (18-35 mg/cm/sup 2//year) reflect the dilution of these high-productivity waters by continentally derived detritus. Comparison of calculated accumulation rates with rates determined for similarly aged strata of the Santa Barbara basin reveals that silica and terrigenous debris were accumulating at higher rates within the Santa Maria basin. These differences are due to the complex interplay of tectonics and oceanographics and to the proximity of the basins relative to the strandline.

  4. Rate of tree carbon accumulation increases continuously with tree size.

    PubMed

    Stephenson, N L; Das, A J; Condit, R; Russo, S E; Baker, P J; Beckman, N G; Coomes, D A; Lines, E R; Morris, W K; Rüger, N; Alvarez, E; Blundo, C; Bunyavejchewin, S; Chuyong, G; Davies, S J; Duque, A; Ewango, C N; Flores, O; Franklin, J F; Grau, H R; Hao, Z; Harmon, M E; Hubbell, S P; Kenfack, D; Lin, Y; Makana, J-R; Malizia, A; Malizia, L R; Pabst, R J; Pongpattananurak, N; Su, S-H; Sun, I-F; Tan, S; Thomas, D; van Mantgem, P J; Wang, X; Wiser, S K; Zavala, M A

    2014-03-01

    Forests are major components of the global carbon cycle, providing substantial feedback to atmospheric greenhouse gas concentrations. Our ability to understand and predict changes in the forest carbon cycle--particularly net primary productivity and carbon storage--increasingly relies on models that represent biological processes across several scales of biological organization, from tree leaves to forest stands. Yet, despite advances in our understanding of productivity at the scales of leaves and stands, no consensus exists about the nature of productivity at the scale of the individual tree, in part because we lack a broad empirical assessment of whether rates of absolute tree mass growth (and thus carbon accumulation) decrease, remain constant, or increase as trees increase in size and age. Here we present a global analysis of 403 tropical and temperate tree species, showing that for most species mass growth rate increases continuously with tree size. Thus, large, old trees do not act simply as senescent carbon reservoirs but actively fix large amounts of carbon compared to smaller trees; at the extreme, a single big tree can add the same amount of carbon to the forest within a year as is contained in an entire mid-sized tree. The apparent paradoxes of individual tree growth increasing with tree size despite declining leaf-level and stand-level productivity can be explained, respectively, by increases in a tree's total leaf area that outpace declines in productivity per unit of leaf area and, among other factors, age-related reductions in population density. Our results resolve conflicting assumptions about the nature of tree growth, inform efforts to undertand and model forest carbon dynamics, and have additional implications for theories of resource allocation and plant senescence.

  5. Rate of tree carbon accumulation increases continuously with tree size

    NASA Astrophysics Data System (ADS)

    Stephenson, N. L.; Das, A. J.; Condit, R.; Russo, S. E.; Baker, P. J.; Beckman, N. G.; Coomes, D. A.; Lines, E. R.; Morris, W. K.; Rüger, N.; Álvarez, E.; Blundo, C.; Bunyavejchewin, S.; Chuyong, G.; Davies, S. J.; Duque, Á.; Ewango, C. N.; Flores, O.; Franklin, J. F.; Grau, H. R.; Hao, Z.; Harmon, M. E.; Hubbell, S. P.; Kenfack, D.; Lin, Y.; Makana, J.-R.; Malizia, A.; Malizia, L. R.; Pabst, R. J.; Pongpattananurak, N.; Su, S.-H.; Sun, I.-F.; Tan, S.; Thomas, D.; van Mantgem, P. J.; Wang, X.; Wiser, S. K.; Zavala, M. A.

    2014-03-01

    Forests are major components of the global carbon cycle, providing substantial feedback to atmospheric greenhouse gas concentrations. Our ability to understand and predict changes in the forest carbon cycle--particularly net primary productivity and carbon storage--increasingly relies on models that represent biological processes across several scales of biological organization, from tree leaves to forest stands. Yet, despite advances in our understanding of productivity at the scales of leaves and stands, no consensus exists about the nature of productivity at the scale of the individual tree, in part because we lack a broad empirical assessment of whether rates of absolute tree mass growth (and thus carbon accumulation) decrease, remain constant, or increase as trees increase in size and age. Here we present a global analysis of 403 tropical and temperate tree species, showing that for most species mass growth rate increases continuously with tree size. Thus, large, old trees do not act simply as senescent carbon reservoirs but actively fix large amounts of carbon compared to smaller trees; at the extreme, a single big tree can add the same amount of carbon to the forest within a year as is contained in an entire mid-sized tree. The apparent paradoxes of individual tree growth increasing with tree size despite declining leaf-level and stand-level productivity can be explained, respectively, by increases in a tree's total leaf area that outpace declines in productivity per unit of leaf area and, among other factors, age-related reductions in population density. Our results resolve conflicting assumptions about the nature of tree growth, inform efforts to undertand and model forest carbon dynamics, and have additional implications for theories of resource allocation and plant senescence.

  6. Rate of tree carbon accumulation increases continuously with tree size.

    PubMed

    Stephenson, N L; Das, A J; Condit, R; Russo, S E; Baker, P J; Beckman, N G; Coomes, D A; Lines, E R; Morris, W K; Rüger, N; Alvarez, E; Blundo, C; Bunyavejchewin, S; Chuyong, G; Davies, S J; Duque, A; Ewango, C N; Flores, O; Franklin, J F; Grau, H R; Hao, Z; Harmon, M E; Hubbell, S P; Kenfack, D; Lin, Y; Makana, J-R; Malizia, A; Malizia, L R; Pabst, R J; Pongpattananurak, N; Su, S-H; Sun, I-F; Tan, S; Thomas, D; van Mantgem, P J; Wang, X; Wiser, S K; Zavala, M A

    2014-03-01

    Forests are major components of the global carbon cycle, providing substantial feedback to atmospheric greenhouse gas concentrations. Our ability to understand and predict changes in the forest carbon cycle--particularly net primary productivity and carbon storage--increasingly relies on models that represent biological processes across several scales of biological organization, from tree leaves to forest stands. Yet, despite advances in our understanding of productivity at the scales of leaves and stands, no consensus exists about the nature of productivity at the scale of the individual tree, in part because we lack a broad empirical assessment of whether rates of absolute tree mass growth (and thus carbon accumulation) decrease, remain constant, or increase as trees increase in size and age. Here we present a global analysis of 403 tropical and temperate tree species, showing that for most species mass growth rate increases continuously with tree size. Thus, large, old trees do not act simply as senescent carbon reservoirs but actively fix large amounts of carbon compared to smaller trees; at the extreme, a single big tree can add the same amount of carbon to the forest within a year as is contained in an entire mid-sized tree. The apparent paradoxes of individual tree growth increasing with tree size despite declining leaf-level and stand-level productivity can be explained, respectively, by increases in a tree's total leaf area that outpace declines in productivity per unit of leaf area and, among other factors, age-related reductions in population density. Our results resolve conflicting assumptions about the nature of tree growth, inform efforts to undertand and model forest carbon dynamics, and have additional implications for theories of resource allocation and plant senescence. PMID:24429523

  7. Rate of tree carbon accumulation increases continuously with tree size

    USGS Publications Warehouse

    Stephenson, N.L.; Das, A.J.; Condit, R.; Russo, S.E.; Baker, P.J.; Beckman, N.G.; Coomes, D.A.; Lines, E.R.; Morris, W.K.; Rüger, N.; Álvarez, E.; Blundo, C.; Bunyavejchewin, S.; Chuyong, G.; Davies, S.J.; Duque, Á.; Ewango, C.N.; Flores, O.; Franklin, J.F.; Grau, H.R.; Hao, Z.; Harmon, M.E.; Hubbell, S.P.; Kenfack, D.; Lin, Y.; Makana, J.-R.; Malizia, A.; Malizia, L.R.; Pabst, R.J.; Pongpattananurak, N.; Su, S.-H.; Sun, I-F.; Tan, S.; Thomas, D.; van Mantgem, P.J.; Wang, X.; Wiser, S.K.; Zavala, M.A.

    2014-01-01

    Forests are major components of the global carbon cycle, providing substantial feedback to atmospheric greenhouse gas concentrations. Our ability to understand and predict changes in the forest carbon cycle—particularly net primary productivity and carbon storage—increasingly relies on models that represent biological processes across several scales of biological organization, from tree leaves to forest stands. Yet, despite advances in our understanding of productivity at the scales of leaves and stands, no consensus exists about the nature of productivity at the scale of the individual tree, in part because we lack a broad empirical assessment of whether rates of absolute tree mass growth (and thus carbon accumulation) decrease, remain constant, or increase as trees increase in size and age. Here we present a global analysis of 403 tropical and temperate tree species, showing that for most species mass growth rate increases continuously with tree size. Thus, large, old trees do not act simply as senescent carbon reservoirs but actively fix large amounts of carbon compared to smaller trees; at the extreme, a single big tree can add the same amount of carbon to the forest within a year as is contained in an entire mid-sized tree. The apparent paradoxes of individual tree growth increasing with tree size despite declining leaf-level and stand-level productivity can be explained, respectively, by increases in a tree’s total leaf area that outpace declines in productivity per unit of leaf area and, among other factors, age-related reductions in population density. Our results resolve conflicting assumptions about the nature of tree growth, inform efforts to understand and model forest carbon dynamics, and have additional implications for theories of resource allocation and plant senescence.

  8. Rate of production, dissolution and accumulation of biogenic solids in the ocean

    NASA Technical Reports Server (NTRS)

    Arrhenius, G.

    1988-01-01

    The equatorial current system, by its response to global circulation changes, provides a unique recording mechanism for long range climatic oscillations. A permanent record of the changes in rate of upwelling and organic production is generated in the equatorial deep sea sediments, particularly by such biogenic components which are unaffected by secondary dissolution. In order to determine the rates of accumulation of various sedimentary components, a reliable differential measurement of age of the strata must be obtained. Various approaches to this problem are reviewed, and sources of error discussed. Secondary dissolution of calcium carbonate introduces a substantial and variable difference between the dissolution-modified, and hence a priori unknown, rate of deposition on one hand and the rate of accumulation, derivable from the observed concentration, on the other. The cause and magnitude of these variations are of importance, particularly since some current dating schemes are based on assumed constancy in the rate of accumulation of this and, in some cases, also all other sedimentary components. The concepts used in rate evaluation are discussed with emphasis on the difference between the state of dissolution, an observable property of the sediment, and the rate of dissolution, a parameter that requires deduction of the carbonate fraction dissolved, and of the time differential. As a most likely cause of the enhanced state of dissolution of the interglacial carbonate sediments is proposed the lowered rates of biogenic production and deposition, which cause longer exposure of the carbonate microfossils to corrosion in the bioturbated surface layer of the sediment. Historical perspective is included in the discussion in view of the dedication of the Symposium to Hans Pettersson, the leader of the Swedish Deep Sea Expedition 1947-1948, an undertaking that opened a new era in deep sea research and planetary dynamics.

  9. Myeloid-derived suppressor cells accumulate in the liver site after sepsis to induce immunosuppression.

    PubMed

    Ren, Dongping; Bi, Qi; Li, Li; Gao, Yongqiang; Liang, Yu; Li, Yingju; Liu, Jun; Peng, Li; Xiao, Jianhua

    2012-09-01

    Myeloid-derived suppressor cells (MDSCs) play a major role in modulating immune response, but only a few reports focused on MDSCs in the liver of sepsis states. Here, we investigated the changes in MDSCs in liver of the cecal ligation and puncture (CLP) mice. The results of flow cytometry showed that MDSCs accumulate in the liver site of mice after CLP for 7days (CLP7d model mice) and settled to the livers of both normal and LPS stimulated mice. In vitro experiment showed a strong suppressive effect of MDSCs on the proliferation of CD4+ T lymphocytes of spleen. Furthermore, adoptive transfer of the liver MDSCs from CLP7d miceincreased the survival rate of acute hepatic failure (AHF) model mice in vivo. In conclusion, our data suggest that sepsis-induced liver MDSCs may have exert a key role in maintaining the immune homoeostasis in liver during the sepsis state.

  10. Accumulation rates from central North Greenland during the past 700 year

    NASA Astrophysics Data System (ADS)

    Karlsson, Nanna B.; Eisen, Olaf; Nielsen, Lisbeth T.; Kipfstuhl, Sepp; Freitag, Johannes; Paden, John D.; Dahl-Jensen, Dorthe; Winter, Anna; Wilhelms, Frank

    2016-04-01

    A key variable when interpreting the evolution and mass loss from polar ice sheets is the input from the surface mass balance. While ice core records contain information on past accumulation rates, they always only provide information for a single location. Here, we present spatially distributed accumulation rates from central northern Greenland, specifically the area between the NEEM (North Greenland Eemian Drilling) and NGRIP (North Greenland Ice Core Project) ice core drill sites. The accumulation rates have been reconstructed using ice-penetrating radar, firn core measurements and inverse methods, and we are able to retrieve both spatial and temporal changes in the accumulation over an area spanning 300 km by 300 km. We investigate the stability of the accumulation pattern over the past several hundred years, and we address the question of how well the measured accumulation rates at the ice core sites capture the regional variations in accumulation. We find that while the accumulation rates at NEEM have been stable for the past 700 years, the NGRIP site has experienced fluctuations in accumulation rate. We interpret this as an indication of shifts in the dominating weather pattern over the ice divide in central North Greenland.

  11. Spatial variation in rates of carbon and nitrogen accumulation in a boreal bog

    SciTech Connect

    Ohlson, M.; Oekland, R.H.

    1998-12-01

    Although previous studies hint at the occurrence of substantial spatial variation in the accumulation rates of C and N in bogs, the extent to which rates may vary on high-resolution spatial and temporal scales is not known. A main reason for the lack of knowledge is that it is problematic to determine the precise age of peat at a given depth. The authors determined rates of carbon and nitrogen accumulation in the uppermost decimeters of a bog ecosystem using the pine method, which enables accurate dating of surface peat layers. They combined accumulation data with numerical and geostatistical analyses of the recent vegetation to establish the relationship between bog vegetation and rate of peat accumulation. Use of a laser technique for spatial positioning of 151 age-determined peat cores within a 20 x 20 m plot made it possible to give the first tine-scaled account of spatial and temporal variation in rates of mass, carbon, and nitrogen accumulation during the last century. Rates of C and N accumulation were highly variable at all spatial scales studied. For example, after {approximately}125 yr of peat growth, C and N accumulation varied by factors of five and four, respectively, from 25 to 125 g/dm{sup 2} for C, and from 0.7 to 2.6 g/dm{sup 2} for N. It takes 40 yr of peat accumulation before significant amounts of C are lost through decay. Hummocks built up by Sphagnum fuscum and S. rubellum were able to maintain average rates of C accumulation that exceed 2 g{center_dot}dm{sup {minus}2}{center_dot} yr{sup {minus}1} during 50 yr of growth. The authors argue that data on spatial variation in rates of C accumulation are necessary to understand the role of boreal peatlands in the greenhouse effect and global climate.

  12. Comparison of elemental accumulation rates between ferromanganese deposits and sediments in the South Pacific Ocean

    USGS Publications Warehouse

    Kraemer, T.; Schornick, J.C.

    1974-01-01

    Rates of accumulation of Fe and Mn, as well as Cu, Ni, Co, Pb, Zn, Hg, U and Th have been determined for five ferromanganese deposits from four localities in the South Pacific Ocean. Manganese is accumulating in nodules and crusts at a rate roughly equivalent to that found to be accumulating in sediments in the same area. Iron shows a deficiency in accumulation in nodules and crusts with respect to sediments, especially near the continents, but also in the central and south-central Pacific. Copper is accumulating in nodules and crusts at a rate one order of magnitude less than the surrounding sediments. This is interpreted as meaning that most of the Mn is supplied as an authigenic phase to both sediments and nodules while Fe is supplied mostly by ferromanganese micro-nodules and by detrital and adsorbed components of sediments; and Cu is enriched in sediments relative to nodules and crusts most probably through biological activity. ?? 1974.

  13. Carbon and Nitrogen Accumulation Rates in Salt Marshes in Oregon, USA

    EPA Science Inventory

    Two important ecosystem services of wetlands are carbon sequestration and filtration of nutrients and particulates. We quantified the carbon and nitrogen accumulation rates in salt marshes at 135 plots distributed across eight estuaries located in Oregon, USA. Net carbon and ...

  14. Geographic variability in organic carbon stock and accumulation rate in sediments of East and Southeast Asian seagrass meadows

    NASA Astrophysics Data System (ADS)

    Miyajima, Toshihiro; Hori, Masakazu; Hamaguchi, Masami; Shimabukuro, Hiromori; Adachi, Hiroshi; Yamano, Hiroya; Nakaoka, Masahiro

    2015-04-01

    Organic carbon (OC) stored in the sediments of seagrass meadows has been considered a globally significant OC reservoir. However, the sparsity and regional bias of studies on long-term OC accumulation in coastal sediments have limited reliable estimation of the capacity of seagrass meadows as a global OC sink. We evaluated the amount and accumulation rate of OC in sediment of seagrass meadows and adjacent areas in East and Southeast Asia. In temperate sites, the average OC concentration in the top 30 cm of sediment was higher in seagrass meadows (780-1080 mmol g-1) than in sediments without seagrass cover (52-430 mmol g-1). The average OC in the top 30 cm of subtropical and tropical seagrass meadow sediments ranged from 140 to 440 mmol g-1. Carbon isotope mass balancing suggested that the contribution of seagrass-derived carbon to OC stored in sediments was often relatively minor (temperate: 10-40%; subtropical: 35-82%; tropical: 4-34%) and correlated to the habitat type, being particularly low in estuarine habitats. Stock of OC in the top meter of sediment of all the studied meadows ranged from 38 to 120 Mg ha-1. The sediment accumulation rates were estimated by radiocarbon dating of six selected cores (0.32-1.34 mm yr-1). The long-term OC accumulation rates calculated from the sediment accumulation rate and the top 30 cm average OC concentration for the seagrass meadows (24-101 kg ha-1 yr-1) were considerably lower than the OC accumulation rates previously reported for Mediterranean Posidonia oceanica meadows (580 kg ha-1 yr-1 on average). Current estimates for the global carbon sink capacity of seagrass meadows, which rely largely on Mediterranean studies, may be considerable overestimations.

  15. Using transplants to measure accumulation rates of epiphytic bryophytes in forests of western Oregon

    USGS Publications Warehouse

    Rosso, A.L.; Muir, Patricia S.; Rambo, T.

    2001-01-01

    We sought a simple and effective transplant method that could be used to measure biomass accumulation rates of epiphytic bryophytes. Trials were carried out in the Pseudotsuga menziesii-dominated forests of western Oregon. We tested multiple transplant methods over a 13-month period while comparing accumulation rates of Antitrichia curtipendula (Hedw.) Brid. and Isothecium myosuroides Brid. among an old-growth stand, a young stand, and a recent clearcut. In our study area, Antitrichia is considered to be an old-growth associate while Isothecium is a more ubiquitous species. Methods tested included containment in net bags, containment in hairnets, and directly tying mats to substrates. Three sizes of transplants were tested with both natural and inert artificial substrates. Transplants of approximately five g enclosed in plastic net bags and tied to either natural or artificial substrates worked well for our purposes. Only minor differences were found in mean accumulation rates between the old growth and young stand, though variation in accumulation rates was higher in the old growth. Neither species appeared capable of surviving in the clearcut. Antitrichia accumulated biomass 60% faster in the canopy than in the understory on average. Antitrichia also accumulated at a faster rate than Isothecium, with mean 13-month biomass increases of 11.8 and 3.7% respectively for 5 g transplants in the understory. Our results suggest that Antitrichia's association with old growth may be due more to dispersal or establishment limitations than to a decreased ability to grow in young stands.

  16. Accumulation rates in Northwest Greenland from continuous GPR profiling along the Greenland Inland Traverse

    NASA Astrophysics Data System (ADS)

    Hawley, R. L.; Courville, Z.; Kehrl, L. M.; Lutz, E.; Osterberg, E. C.; Overly, T. B.; Wong, G. J.

    2012-12-01

    Snow accumulation is one of the fundamental parameters governing the mass balance of the Greenland Ice Sheet. While many point measurements of accumulation exist from shallow and deep ice cores, there are few spatially extensive and continuous records of accumulation in Greenland, particularly in the northwest quadrant. In April and May 2011, the Greenland Inland Traverse traveled via tractor train from Thule Air Base in northwest Greenland to Summit Station, at the center of the ice sheet. The science team on the traverse collected both point and profile measurements along the route. Kinematic GPS and 400 MHz GPR profiles provide geolocated subsurface stratigraphy. Density measurements from snowpits and shallow cores on the profile allow us to determine the true depth of radar reflecting horizons, commonly interpreted to be isochrons. We use three ice cores collected at the beginning, the end, and roughly the middle of the route to date horizons. We traced strong reflecting horizons along the entire route. From the combination of dated, traced horizons, density measurements, and position measurements, we determine accumulation rates continuously along the traverse route. We find our traverse route begins in a high-accumulation area, and accumulation decreases as we cross to the east side of the summit ridge. Accumulation then climbs again as we approach summit (directly on the summit ridge). We compare our accumulation rates with previous studies, both measurements (traverse and point measurements) and models.

  17. Constraints on the Recent Rate of Lunar Regolith Accumulation from Diviner Observations

    NASA Technical Reports Server (NTRS)

    Ghent, R. R.; Hayne, P. O.; Bandfield, J. L.; Campbell, B. A.; Carter, L. M.

    2012-01-01

    Many large craters on the lunar nearside show radar CPR signatures consistent with the presence of blocky ejecta blankets, to distances pre dicted to be covered by continuous ejecta. However, most of these sur faces show limited enhancements in both derived rock abundance and rock-free regolith temperatures calculated from Diviner nighttime infrar ed observations. This indicates that the surface rocks are covered by a layer of thermally insulating regolith material. By matching the results of one-dimensional thermal models to Diviner nighttime temperat ures, we have constrained the thermophysical properties of the upper regolith, and the thickness of regolith overlying proximal ejecta. We find that for all of the regions surveyed (all in the nearside highla nds), the nighttime cooling curves are best fit by a density profile that varies exponentially with depth, consistent with a linear mixture of rocks and regolith fines, with increasing rock content with depth . Our results show significant spatial variations in the density e-folding depth, H, among young crater ejecta regions, indicating differen ces in the thickness of accumulated regolith. However, away from youn g craters, the average regional "equilibrium" value of H (Heq) is remarkably consistent, and is on the order of 5 cm. As expected, near-rim ejecta associated with young craters show lower values of H, indicating a high rock content in the shallow subsurface; for older craters, the average value of H approaches the regional value of Heq. Calculat ed H values for young craters show a clear correlation with published ages, providing the first observational constraint on the recent rate of lunar regolith accumulation. In addition, this result may help to resolve the apparent discrepancy between ages calculated from small crater counts on melt ponds versus counts on continuous ejecta (e.g., King crater; Ashley et al., 2011, LPSC 42, abstract 2437). This method could, in principle, be extended to other

  18. Topographic control and accumulation rate of some Holocene coral reefs: south Florida and Dry Tortugas

    USGS Publications Warehouse

    Shinn, E.A.; Hudson, J.H.; Halley, R.B.; Lidz, B.H.; Taylor, D.L.

    1977-01-01

    Core drilling and examination of underwater excavation on 6 reef sites in south Florida and Dry Tortugas revealed that underlying topography is the major factor controlling reef morphology. Carbon-14 dating on coral recovered from cores enables calculation of accumulation rates. Accumulation rates were found to range from 0.38 m/1000 years in thin Holocene reefs to as much as 4.85 m/1000 years in thicker buildups. Cementation and alteration of corals were found to be more pronounced in areas of low buildup rates than in areas of rapid accumulation rates. Acropora palmata, generally considered the major reef builder in Florida, was found to be absent in most reefs drilled. At Dry Tortugas, the more than 13-meter thick Holocene reef did not contain A. palmata. The principal reef builders in this outer reef are the same as those which built the Pleistocene Key Largo formation, long considered to be fossilized patch reef complex.

  19. Radiocarbon dating, chronologic framework, and changes in accumulation rates of holocene estuarine sediments from Chesapeake Bay

    USGS Publications Warehouse

    Colman, Steven M.; Baucom, P.C.; Bratton, J.F.; Cronin, T. M.; McGeehin, J.P.; Willard, D.; Zimmerman, A.R.; Vogt, P.R.

    2002-01-01

    Rapidly accumulating Holocene sediments in estuaries commonly are difficult to sample and date. In Chesapeake Bay, we obtained sediment cores as much as 20 m in length and used numerous radiocarbon ages measured by accelarator mass spectrometry methods to provide the first detailed chronologies of Holocene sediment accumulation in the bay. Carbon in these sediments is a complex mixture of materials from a variety of sources. Analyses of different components of the sediments show that total organic carbon ages are largely unreliable, because much of the carbon (including coal) has been transported to the bay from upstream sources and is older than sediments in which it was deposited. Mollusk shells (clams, oysters) and foraminifera appear to give reliable results, although reworking and burrowing are potential problems. Analyses of museum specimens collected alive before atmospheric nuclear testing suggest that the standard reservoir correction for marine samples is appropriate for middle to lower Chesapeake Bay. The biogenic carbonate radiocarbon ages are compatible with 210 Pb and 137 Cs data and pollen stratigraphy from the same sites. Post-settlement changes in sediment transport and accumulation is an important environmental issue in many estuaries, including the Chesapeake. Our data show that large variations in sediment mass accumulation rates occur among sites. At shallow water sites, local factors seem to control changes in accumulation rates with time. Our two relatively deep-water sites in the axial channel of the bay have different long-term average accumulation rates, but the history of sediment accumulation at these sites appears to reflect overall conditions in the bay. Mass accumulation rates at the two deep-water sites rapidly increased by about fourfold coincident with widespread land clearance for agriculture in the Chesapeake watershed.

  20. Impact Of Particle Agglomeration On Accumulation Rates In The Glass Discharge Riser Of HLW Melter

    SciTech Connect

    Kruger, A. A.; Rodriguez, C. A.; Matyas, J.; Owen, A. T.; Jansik, D. P.; Lang, J. B.

    2012-11-12

    The major factor limiting waste loading in continuous high-level radioactive waste (HLW) melters is an accumulation of particles in the glass discharge riser during a frequent and periodic idling of more than 20 days. An excessive accumulation can produce robust layers a few centimeters thick, which may clog the riser, preventing molten glass from being poured into canisters. Since the accumulation rate is driven by the size of particles we investigated with x-ray microtomography, scanning electron microscopy, and image analysis the impact of spinel forming components, noble metals, and alumina on the size, concentration, and spatial distribution of particles, and on the accumulation rate. Increased concentrations of Fe and Ni in the baseline glass resulted in the formation of large agglomerates that grew over the time to an average size of ~185+-155 {mu}m, and produced >3 mm thick layer after 120 h at 850 deg C. The noble metals decreased the particle size, and therefore significantly slowed down the accumulation rate. Addition of alumina resulted in the formation of a network of spinel dendrites which prevented accumulation of particles into compact layers.

  1. Character, paleoenvironment, rate of accumulation, and evidence for seismic triggering of Holocene turbidites, Canada Abyssal Plain, Arctic Ocean

    USGS Publications Warehouse

    Grantz, A.; Phillips, R.L.; Mullen, M.W.; Starratt, S.W.; Jones, Glenn A.; Naidu, A.S.; Finney, B.P.

    1996-01-01

    Four box cores and one piston core show that Holocene sedimentation on the southern Canada Abyssal Plain for the last 8010??120 yr has consisted of a continuing rain of pelagic organic and ice-rafted elastic sediment with a net accumulation rate during the late Holocene of ???10 mm/1000 yr, and episodically emplaced turbidites 1-5 m thick deposited at intervals of 830 to 3450 yr (average 2000 yr). The average net accumulation rate of the mixed sequence of turbidites and thin pelagite interbeds in the cores is about 1.2 m/1000 yr. Physiography suggests that the turbidites originated on the Mackenzie Delta or its clinoform, and ??13C values of -27 to - 25??? in the turbidites are compatible with a provenance on a delta. Extant displaced neritic and lower slope to basin plain calcareous benthic foraminifers coexist in the turbidite units. Their joint occurence indicates that the turbidites originated on the modern continental shelf and entrained sediment from the slope and rise enroute to their final resting place on the Canada Abyssal Plain. The presence of Middle Pleistocene diatoms in the turbidites suggests, in addition, that the turbidites may have originated in shallow submarine slides beneath the upper slope or outer shelf. Small but consistent differences in organic carbon content and ??13C values between the turbidite units suggest that they did not share an identical provenance, which is at least compatible with an origin in slope failures. The primary provenance of the ice-rafted component of the pelagic beds was the glaciated terrane of northwestern Canada; and the provenance of the turbidite units was Pleistocene and Holocene sedimentary deposits on the outer continental shelf and upper slope of the Mackenzie Delta. Largely local derivation of the sediment of the Canada Abyssal Plain indicates that sediment accumulation rates in the Arctic Ocean are valid only for regions with similar depositional sources and processes, and that these rates cannot be

  2. Atmospheric Pb and Ti accumulation rates from Sphagnum moss: dependence upon plant productivity.

    PubMed

    Kempter, H; Krachler, M; Shotyk, W

    2010-07-15

    The accumulation rates of atmospheric Pb and Ti were obtained using the production rates of Sphagnum mosses collected in four ombrotrophic bogs from two regions of southern Germany: Upper Bavaria (Oberbayern, OB) and the Northern Black Forest (Nordschwarzwald, NBF). Surfaces of Sphagnum carpets were marked with plastic mesh and one year later the production of plant matter was harvested. Metal concentrations were determined in acid digests using sector field ICP-MS employing well established analytical procedures. Up to 12 samples (40 x 40 cm) were collected per site, and 6-10 sites were investigated per bog. Variations within a given sampling site were in the range 2.3-4x for Pb concentrations, 1.8-2.5x for Ti concentrations, 3-8.3x for Pb/Ti, 5.6-7.8x for Pb accumulation rates, and 2.3-6.4x for Ti accumulation rates. However, the median values of these parameters for the sites (6-10 per bog) were quite consistent. The mosses from the bogs in NBF exhibited significantly greater productivity (187-202 g m(-2) a(-1)) compared to the OB peat bogs (71-91 g m(-2) a(-1)), and these differences had a pronounced effect on the Pb and Ti accumulation rates. Highly productive mosses showed no indication of a "dilution effect" of Pb or Ti concentrations, suggesting that more productive plants were simply able to accumulate more particles from the air. The median rates of net Pb accumulation by the mosses are in excellent agreement with the fluxes obtained by direct atmospheric measurements at nearby monitoring stations in both regions (EMEP and MAPESI data). PMID:20545344

  3. In vitro Accumulation of Polyphenols in Tea Callus Derived from Anther

    PubMed Central

    Chevala, Naga Pavan Kumar; Chevala, Naga Thirumalesh; Dhanakodi, Kirubakaran; Nadendla, Rama Rao; Nagarathna, Chandrashekara Krishnappa

    2016-01-01

    Background: Tea is an economic important crop with high medicinal value due to rich polyphenols content. In the present research we studied the accumulation of polyphenols of in vitro regenerated callus from anthers. Objective: Callus induction of tea anthers and in vitro accumulation of phenolic compounds from the anther-derived callus. Materials and Methods: Standardization of callus induction for tea anthers. In vitro generated callus was screened for in vivo accumulation of catechins and its isomers were screened by FC reagent staining technique. The methanol extract of dry and green callus obtained were estimated qualitatively by Fourier transform infrared spectroscopy (FTIR)–alternative total reflection (ATR) and quantitatively by HPLC method. Results: Anthers inoculated on half strength MS media fortified with 2,4-dichloro acetic acid (2 mg/L), Kn (1 mg/L), and BAP (1 mg/L) induced callus under photoperiod of 9:15 h light. The in vivo histochemical studies revealed the accumulation of polyphenols in the callus. The in vitro generated fresh and dry callus were used for extraction and screened for accumulated polyphenols [galic acid, (+)-catechin (C), (−)-epicatechin, (−)-epigallocatechin, (−)-epigallocatechin gallate, (−)-gallocatechins, (−)-epicatechin gallate] were estimated qualitatively by FTIR–ATR method and quantitatively by HPLC method. Conclusion: The FC staining technique used here helps in localization of polyphenol compounds accumulation in the tissues by instant microscopic studies. The study have scope in large-scale isolation of various medicinally important flavonol by using anther culture. Abbreviations used: HPLC: high pressure liquid chromatography; FTIR: Fourier transform infrared spectroscopy; 2,4-D: 2,4-dichloro acetic acid; BAP: N6-benzyl amino purine; kn: kinetin PMID:27761066

  4. The accumulation rate of meteorite falls at the earth's surface - The view from Roosevelt County, New Mexico

    NASA Technical Reports Server (NTRS)

    Zolensky, Michael E.; Wells, Gordon L.; Rendell, Helen M.

    1990-01-01

    The discovery of 154 meteorite fragments within an 11-sq km area of wind-excavated basins in Roosevelt County, New Mexico, permits a new calculation of the accumulation rate of meteorite falls at the earth's surface. Thermoluminescence dating of the coversand unit comprising the prime recovery surface suggests the maximum terrestrial age of the meteorites to be about 16.0 ka. The 68 meteorite fragments subjected to petrological analyses represent a minimum of 49 individual falls. Collection bias has largely excluded carbonaceous chondrites and achondrites, requiring the accumulation rate derived from the recovered samples to be increased by a factor of 1.25. Terrestrial weathering destroying ordinary chondrites can be modeled as a first-order decay process with an estimated half-life of 3.5 + or - 1.9 ka on the semiarid American High Plains. Having accounted for the age of the recovery surface, area of field searches, pairing of finds, collection bias and weathering half-life, an accumulation rate of 940 falls/a per 10 to the 6th sq km is calculated for falls greater than 10 g total mass. This figure exceeds the best-constrained previous estimate by more than an order of magnitude. One possible reason for this disparity may be the extraordinary length of the fall record preserved in the surficial geology of Roosevelt County. The high accumulation rate determined for the past 16 ka may point to the existence of periods when the meteorite fall rate was significantly greater than at present.

  5. Patterns and rates of organic carbon accumulation in recent pelagic and hemipelagic marine environments

    SciTech Connect

    Cwienk, D.; Leinen, M.S.; Arthur, M.A.

    1987-05-01

    Maps were constructed for the global distribution of percent organic carbon, sedimentation rates, bulk sediment accumulation rates, and organic carbon accumulation rates (OCARs), exclusive of the continental shelves, on the basis of both published and some new data from sediment cores. The OCAR data base uses only cores in which stratigraphic resolution is sufficient to define a mean sedimentation rate for the last 12,000 years and from which bulk density measurements are available or can be calculated using established relationships to carbonate content. Data coverage was adequate for much of the Pacific Ocean basin but was somewhat sparse for the Atlantic, Indian, and Southern oceans. Nonetheless, basic patterns of organic carbon accumulation can be observed from the maps. As expected, the OCARs are highest in the equatorial Pacific, where surface-water productivity is highest, and low under the central gyre regions where surface organic carbon flux and total sediment flux are both low. Higher OCARs also occur in more rapidly accumulating sediments near the continental margins, partly as a function of the enhanced preservation of labile carbon that results from higher sedimentation rates and, in some cases, from higher productivity in surface water masses or increased flux of terrigenous organic matter. The patterns of OCAR are similar, in general, to patterns of accumulation of biogenic opal, thereby reinforcing the link between biological productivity and OCARs. An analysis of the global impact of pelagic-hemipelagic OCARs, using this data base, suggests that no more than 0.21 x 10/sup 14/ gOC/year accumulate over the deep-sea floor at present - only about 16% of the estimated global annual organic carbon burial. However, the deep-sea organic carbon sink may have been more important in the past.

  6. Incorporation of radiometric tracers in peat and implications for estimating accumulation rates.

    PubMed

    Hansson, Sophia V; Kaste, James M; Olid, Carolina; Bindler, Richard

    2014-09-15

    Accurate dating of peat accumulation is essential for quantitatively reconstructing past changes in atmospheric metal deposition and carbon burial. By analyzing fallout radionuclides (210)Pb, (137)Cs, (241)Am, and (7)Be, and total Pb and Hg in 5 cores from two Swedish peatlands we addressed the consequence of estimating accumulation rates due to downwashing of atmospherically supplied elements within peat. The detection of (7)Be down to 18-20 cm for some cores, and the broad vertical distribution of (241)Am without a well-defined peak, suggest some downward transport by percolating rainwater and smearing of atmospherically deposited elements in the uppermost peat layers. Application of the CRS age-depth model leads to unrealistic peat mass accumulation rates (400-600 g m(-2) yr(-1)), and inaccurate estimates of past Pb and Hg deposition rates and trends, based on comparisons to deposition monitoring data (forest moss biomonitoring and wet deposition). After applying a newly proposed IP-CRS model that assumes a potential downward transport of (210)Pb through the uppermost peat layers, recent peat accumulation rates (200-300 g m(-2) yr(-1)) comparable to published values were obtained. Furthermore, the rates and temporal trends in Pb and Hg accumulation correspond more closely to monitoring data, although some off-set is still evident. We suggest that downwashing can be successfully traced using (7)Be, and if this information is incorporated into age-depth models, better calibration of peat records with monitoring data and better quantitative estimates of peat accumulation and past deposition are possible, although more work is needed to characterize how downwashing may vary between seasons or years.

  7. Rates and environmental controls of aeolian dust accumulation, Athabasca River Valley, Canadian Rocky Mountains

    NASA Astrophysics Data System (ADS)

    Hugenholtz, Chris H.; Wolfe, Stephen A.

    2010-09-01

    Despite an abundance of sedimentary archives of mineral dust (i.e. loess) accumulations from cold, humid environments, the absence of contemporary process investigations limits paleoenvironmental interpretations in these settings. Dust accumulations measured at Jasper Lake, a seasonally-filled reach of the glacially-fed Athabasca River in the Canadian Rocky Mountains, are some of the highest contemporary rates recorded to date. High deposition rates, including a maximum of 27,632 kg ha -1 month -1, occur during river low-flow periods, but even the lowest deposition rates, occurring during bankfull periods, exceed other contemporary rates of deposition. High rates of dust deposition may be attributed to geomorphic and climatic controls affecting sediment supply, availability and transport, and biologic factors affecting accumulation. Localized confinement of the Jasper River by tributary river alluvial fans has caused channel expansion upstream, and formation of the shallow depositional basin known as Jasper Lake. This localized sedimentary basin, coupled with large seasonal water level fluctuations and suitably high wind speeds, favors seasonal dust production. In addition, a dense source-proximal coniferous forest stand encourages high dust accumulation, via increased aerodynamic roughness and airflow deceleration. The forest stand also appears to act as an efficient dust filter, with the interception and storage of dust by the forest canopy playing a significant role with regards to secondary fallout and sediment accumulation. Overall, these results provide new insights on the environmental controls of dust entrainment and accumulation in cold, humid settings, and help clarify controls on the formation of Holocene river-sourced loess deposits.

  8. Impacts of timber harvesting on historic sediment accumulation rates in the Coos Bay estuary, Oregon

    NASA Astrophysics Data System (ADS)

    Mathabane, N.; Roering, J. J.

    2014-12-01

    The expansion and development of human infrastructure along the coastline of the Pacific Northwest has profound consequences for the habitability and general ecological health of coastal ecosystems. Coos County, one of the most economically critical regions of the Oregon Coast, experienced vigorous timber harvest activity in the aftermath of WWII that declined in the last several decades. This period of extractive land use may have drastically altered the sediment supply in the major catchments of the Coos and Millicoma Rivers and lead to variations in sediment flux into the Coos Bay estuary. Accurate sediment flux histories are critical data for deciphering the relative importance of climate and land use factors such as logging and road construction on sediment production. Reduction of root reinforcement following timber harvest increases the likelihood of shallow landsliding and debris flows. In addition, forest roads increase sediment production due to overland flow and entrainment of fine sediments on hydrologically connected roads. Although these processes have been documented in small watersheds, their compounded effect on estuaries and coastal settings has not been well documented. We use Pb-210 activities derived from sediment cores taken at various locations in the Coos Bay estuary to establish temporal variations in sediment accumulation rates (SARs). Our cores will also be analyzed to assess dissolved oxygen and other proxies for ecosystem functioning. By correlating these SARs with quantitative metrics for timber extraction rate such as board feet per year and qualitative evaluations from historical photos, we propose to document the cumulative effect of historic forest practices. The temporal resolution provided by this technique should allow us to link changes in estuarine sedimentation to changes in land use as well as climatic triggers such as storms. The conclusions of this study will add valuable information regarding the ultimate impact of

  9. Decadal sediment accumulation rates on the floodplain of the Strickland River, Papua New Guinea

    NASA Astrophysics Data System (ADS)

    Swanson, K. M.; Watson, E.; Aalto, R.; Lauer, J. W.; Bera, M. T.; Marshall, A.; Taylor, M.; Apte, S. C.; Dietrich, W. E.

    2006-12-01

    Rate of aggradation and infilling of accommodation space along lowland channels in response to post-glacial sea level rise should depend on sediment supply. The Strickland and Fly Rivers join at just 6 m above sea level, and the Strickland has historically carried about 7 times the sediment load and twice the river discharge as the Fly. Here we test the hypothesis that the lowland Strickland River floodplain should now have a lower sediment trap efficiency (due to more fully developed floodplains) than the middle Fly River, which currently losses about 40% of its load to the floodplain annually. We use mine-derived elevated Pb and Ag in floodplain core samples to determine the rates of sedimentation across the lower Strickland floodplain. Field sampling campaigns were conducted in 1997 and 2003, collecting shallow (less than 1 m) core samples at 5 relatively straight reaches up to 2 km from channel banks (in 1997 and 2003) and from 3 strongly curved sections, as well as from other sites of interest (2003). Various observations indicate that the concentration of particulate metals in overbank floods varies with stage, drought-flood cycles, and through the life of the mine. Sediment accumulation declines across the floodplain from the channel bank with an average rate of about 1.4 cm/yr over the first 1 km. Some sedimentation farther out did occur but is not well defined by our data. Overbank deposition is about 13% of the total load. Sedimentation rate per unit area is perhaps 10 times that measured on the Fly, but lateral migration on the Strickland is sufficiently high (average 5 m/yr) that overbank deposits are returned to the channel, significantly reducing potential net deposition to the floodplain. We conclude that the Strickland, which is nearly 10 times steeper than the Fly, and has much higher overbank deposition rates, nonetheless has less net deposition than the Fly because of the more vigorous lateral migration (which apparently results from the higher

  10. High Rates of Species Accumulation in Animals with Bioluminescent Courtship Displays.

    PubMed

    Ellis, Emily A; Oakley, Todd H

    2016-07-25

    One of the great mysteries of evolutionary biology is why closely related lineages accumulate species at different rates. Theory predicts that populations undergoing strong sexual selection will more quickly differentiate because of increased potential for genetic isolation [1-6]. Whether or not these population genetic processes translate to more species at macroevolutionary scales remains contentious [7]. Here we show that lineages with bioluminescent courtship, almost certainly a sexually selected trait, have more species and faster rates of species accumulation than their non-luminous relatives. In each of ten distantly related animal lineages from insects, crustaceans, annelid worms, and fishes, we find more species in lineages with bioluminescent courtship compared to their sister groups. Furthermore, we find under a Yule model that lineages with bioluminescent courtship displays have significantly higher rates of species accumulation compared to a larger clade that includes them plus non-luminous relatives. In contrast, we do not find more species or higher rates in lineages that use bioluminescence for defense, a function presumably not under sexual selection. These results document an association between the origin of bioluminescent courtship and increased accumulation of species, supporting theory predicting sexual selection increases rates of speciation at macroevolutionary scales to influence global patterns of biodiversity. PMID:27345160

  11. Derivation of rating curve by the Tsallis entropy

    NASA Astrophysics Data System (ADS)

    Singh, Vijay P.; Cui, Huijuan; Byrd, Aaron R.

    2014-05-01

    The stage-discharge relation, often called rating curve, is employed to determine discharge in natural and engineered channels. There are several methods for deriving a rating curve most of which are empirical. It is well recognized that rating curves are subjected to significant uncertainty, yet most of these methods do not have any provision to account for or do not quantify the uncertainty. This study employs the Tsallis entropy for deriving the rating curve, based on two simple constraints: (1) total probability and (2) mean discharge. Parameters of the derived curve are determined with the use of these two constraints. The rating curve is also determined by reparameterization with the use of an entropy parameter. The Tsallis entropy permits a probabilistic characterization of the rating curve and hence the probability density function of discharge underlying the curve. It also permits a quantitative assessment of the uncertainty of discharge obtained from the rating curve. The derived rating curve is found to be in agreement with field data and is also applied to ungaged watersheds. The rating curve is also extended beyond the range of discharge values used in its construction and its validity is then evaluated.

  12. Accumulation of phenylpropanoid derivatives in chitosan-induced cell suspension culture of Cocos nucifera.

    PubMed

    Chakraborty, Moumita; Karun, Anitha; Mitra, Adinpunya

    2009-01-01

    Chitosan-induced elicitation responses of dark-incubated Cocos nucifera (coconut) endosperm cell suspension cultures led to the rapid formation of phenylpropanoid derivatives, which essentially mimics the defense-induced biochemical changes in coconut palm as observed under in vivo conditions. An enhanced accumulation of p-hydroxybenzoic acid as the major wall-bound phenolics was evident. This was followed by p-coumaric acid and ferulic acid. Along with enhanced peroxidases activities in elicited lines, the increase in activities of the early phenylpropanoid pathway enzymes such as, phenylalanine ammonia lyase (PAL), p-coumaroyl-CoA ligase (4CL) and p-hydroxybenzaldehyde dehydrogenase (HBD) in elicited cell cultures were also observed. Furthermore, supplementation of specific inhibitors of PAL, C4H and 4CL in elicited cell cultures led to suppressed accumulation of p-hydroxybenzoic acid, which opens up interesting questions regarding the probable route of the biosynthesis of this phenolic acid in C. nucifera.

  13. Accumulation of phenylpropanoid derivatives in chitosan-induced cell suspension culture of Cocos nucifera.

    PubMed

    Chakraborty, Moumita; Karun, Anitha; Mitra, Adinpunya

    2009-01-01

    Chitosan-induced elicitation responses of dark-incubated Cocos nucifera (coconut) endosperm cell suspension cultures led to the rapid formation of phenylpropanoid derivatives, which essentially mimics the defense-induced biochemical changes in coconut palm as observed under in vivo conditions. An enhanced accumulation of p-hydroxybenzoic acid as the major wall-bound phenolics was evident. This was followed by p-coumaric acid and ferulic acid. Along with enhanced peroxidases activities in elicited lines, the increase in activities of the early phenylpropanoid pathway enzymes such as, phenylalanine ammonia lyase (PAL), p-coumaroyl-CoA ligase (4CL) and p-hydroxybenzaldehyde dehydrogenase (HBD) in elicited cell cultures were also observed. Furthermore, supplementation of specific inhibitors of PAL, C4H and 4CL in elicited cell cultures led to suppressed accumulation of p-hydroxybenzoic acid, which opens up interesting questions regarding the probable route of the biosynthesis of this phenolic acid in C. nucifera. PMID:18448193

  14. Endoproteolysis of presenilin 1 and accumulation of processed derivatives in vivo.

    PubMed

    Thinakaran, G; Borchelt, D R; Lee, M K; Slunt, H H; Spitzer, L; Kim, G; Ratovitsky, T; Davenport, F; Nordstedt, C; Seeger, M; Hardy, J; Levey, A I; Gandy, S E; Jenkins, N A; Copeland, N G; Price, D L; Sisodia, S S

    1996-07-01

    The majority of early-onset cases of familial Alzheimer's disease (FAD) are linked to mutations in two related genes, PS1 and PS2, located on chromosome 14 and 1, respectively. Using two highly specific antibodies against nonoverlapping epitopes of the PS1-encoded polypeptide, termed presenilin 1 (PS1), we document that the preponderant PS1-related species that accumulate in cultured mammalian cells, and in the brains of rodents, primates, and humans are approximately 27-28 kDa N-terminal and approximately 16-17 kDa C-terminal derivatives. Notably, a FAD-linked PS1 variant that lacks exon 9 is not subject to endoproteolytic cleavage. In brains of transgenic mice expressing human PS1, approximately 17 kDa and approximately 27 kDa PS1 derivatives accumulate to saturable levels, and at approximately 1:1 stoichiometry, independent of transgene-derived mRNA. We conclude that PS1 is subject to endoproteolytic processing in vivo. PMID:8755489

  15. Nutritive Value and Herbage Accumulation Rates of Pasture Sown to Grass, Legume, and Chicory

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Planting pastures to mixtures of forages may benefit herbage production; however, wide fluctuations in botanical composition could cause unstable nutritive value. A grazing study was conducted to examine how forage mixture complexity influenced nutritive value and accumulation rate during spring, su...

  16. Marine debris accumulation in the Northwestern Hawaiian Islands: an examination of rates and processes.

    PubMed

    Dameron, Oliver J; Parke, Michael; Albins, Mark A; Brainard, Russell

    2007-04-01

    Large amounts of derelict fishing gear accumulate and cause damage to shallow coral reefs of the Northwestern Hawaiian Islands (NWHI). To facilitate maintenance of reefs cleaned during 1996-2005 removal efforts, we identify likely high-density debris areas by assessing reef characteristics (depth, benthic habitat type, and energy regime) that influence sub-regional debris accumulation. Previously cleaned backreef and lagoonal reefs at two NWHI locations were resurveyed for accumulated debris using two survey methods. Accumulated debris densities and weights were found to be greater in lagoonal reef areas. Sample weight-based debris densities are extrapolated to similar habitats throughout the NWHI using a spatial 'net habitat' dataset created by generalizing IKONOS satellite derivatives for depth and habitat classification. Prediction accuracy for this dataset is tested using historical debris point data. Annual NWHI debris accumulation is estimated to be 52.0 metric tonnes. For planning purposes, individual NWHI atolls/reefs are allotted a proportion of this total. PMID:17217968

  17. Project Hotspot: Linear accumulation rates of late Cenozoic basalt at Kimama, Idaho, and implications for crustal strain and subsidence rates of the central Snake River Plain

    NASA Astrophysics Data System (ADS)

    Rodgers, D. W.; Potter, K. E.; Shervais, J. W.; Champion, D. E.; Duncan, R. A.

    2013-12-01

    Project Hotspot's Kimama drill hole on the Snake River Plain, Idaho recovered a 1912 m thick section of basalt core that ranges in age from ~700 ka to at least 6.14 Ma, based on five 40Ar/39Ar analyses and twenty paleomagnetic age assignments. Fifty-four flow groups comprising 510 individual flows were defined, yielding an average recurrence interval of ~11,400 years between flows. Age-depth analysis indicate that, over thicknesses >150 m and age spans >500 k.y., accumulation rates were constant at 30 m/100 k.y. The existence and persistence of this linear accumulation rate for greater than 5 m.y. documents an external tectonic control on eruption dynamics. One conceptual model relates accumulation rates to horizontal crustal strain, such that far-field extension rate controls the periodicity of dikes that feed basalt flows. In this model, each of the 54 flow groups would have a deep-seated, relatively wide (1-10m) dike that branches upward into a network of narrow (10-100 cm) dikes feeding individual lava flows. Assuming an east-west lateral lava flow extent of up to 50 km, the Kimama data record a steady-state crustal strain rate of 10-9 to 10-10 y-1. This rate is comparable to modern, decadal strain rates measured with GPS in the adjacent Basin & Range province, but exceeds decadal strain rates of zero measured in the eastern Snake River Plain. Linear accumulation rates also provide insight into basalt subsidence history. In this model, the middle-upper crust subsides due to the added weight of lava flows, the added weight of mid-crustal sills/dikes, and thermal contraction in the wake of the Yellowstone hot spot. Isostatic compensation would occur in the (nearly) molten lower crust. Assuming constant surface elevation and a basalt density of 2.6 g/cm3, the lava flow weight would account for 87% of the burial through time, yielding a steady-state "tectonic" subsidence rate of 4 m/100 k.y. attributed to the driving forces of mid-crustal injection and/or thermal

  18. Adipocytes as a Link Between Gut Microbiota-Derived Flagellin and Hepatocyte Fat Accumulation.

    PubMed

    Munukka, Eveliina; Wiklund, Petri; Partanen, Tiina; Välimäki, Sakari; Laakkonen, Eija K; Lehti, Maarit; Fischer-Posovzsky, Pamela; Wabitsch, Martin; Cheng, Sulin; Huovinen, Pentti; Pekkala, Satu

    2016-01-01

    While the role of both elevated levels of circulating bacterial cell wall components and adipose tissue in hepatic fat accumulation has been recognized, it has not been considered that the bacterial components-recognizing adipose tissue receptors contribute to the hepatic fat content. In this study we found that the expression of adipose tissue bacterial flagellin (FLG)-recognizing Toll-like receptor (TLR) 5 associated with liver fat content (r = 0.699, p = 0.003) and insulin sensitivity (r = -0.529, p = 0.016) in humans (n = 23). No such associations were found for lipopolysaccharides (LPS)-recognizing TLR4. To study the underlying molecular mechanisms of these associations, human HepG2 hepatoma cells were exposed in vitro to the conditioned culture media derived from FLG or LPS-challenged human adipocytes. The adipocyte-mediated effects were also compared to the effects of direct HepG2 exposure to FLG and LPS. We found that the media derived from FLG-treated adipocytes stimulated fat accumulation in HepG2 cells, whereas either media derived from LPS-treated adipocytes or direct FLG or LPS exposure did not. This is likely due to that FLG-treatment of adipocytes increased lipolysis and secretion of glycerol, which is known to serve a substrate for triglyceride synthesis in hepatocytes. Similarly, only FLG-media significantly decreased insulin signaling-related Akt phosphorylation, IRS1 expression and mitochondrial respiratory chain ATP5A. In conclusion, our results suggest that the FLG-induced TLR5 activation in adipocytes increases glycerol secretion from adipocytes and decreases insulin signaling and mitochondrial functions, and increases fat accumulation in hepatocytes. These mechanisms could, at least partly, explain the adipose tissue TLR5 expression associated with liver fat content in humans.

  19. Adipocytes as a Link Between Gut Microbiota-Derived Flagellin and Hepatocyte Fat Accumulation.

    PubMed

    Munukka, Eveliina; Wiklund, Petri; Partanen, Tiina; Välimäki, Sakari; Laakkonen, Eija K; Lehti, Maarit; Fischer-Posovzsky, Pamela; Wabitsch, Martin; Cheng, Sulin; Huovinen, Pentti; Pekkala, Satu

    2016-01-01

    While the role of both elevated levels of circulating bacterial cell wall components and adipose tissue in hepatic fat accumulation has been recognized, it has not been considered that the bacterial components-recognizing adipose tissue receptors contribute to the hepatic fat content. In this study we found that the expression of adipose tissue bacterial flagellin (FLG)-recognizing Toll-like receptor (TLR) 5 associated with liver fat content (r = 0.699, p = 0.003) and insulin sensitivity (r = -0.529, p = 0.016) in humans (n = 23). No such associations were found for lipopolysaccharides (LPS)-recognizing TLR4. To study the underlying molecular mechanisms of these associations, human HepG2 hepatoma cells were exposed in vitro to the conditioned culture media derived from FLG or LPS-challenged human adipocytes. The adipocyte-mediated effects were also compared to the effects of direct HepG2 exposure to FLG and LPS. We found that the media derived from FLG-treated adipocytes stimulated fat accumulation in HepG2 cells, whereas either media derived from LPS-treated adipocytes or direct FLG or LPS exposure did not. This is likely due to that FLG-treatment of adipocytes increased lipolysis and secretion of glycerol, which is known to serve a substrate for triglyceride synthesis in hepatocytes. Similarly, only FLG-media significantly decreased insulin signaling-related Akt phosphorylation, IRS1 expression and mitochondrial respiratory chain ATP5A. In conclusion, our results suggest that the FLG-induced TLR5 activation in adipocytes increases glycerol secretion from adipocytes and decreases insulin signaling and mitochondrial functions, and increases fat accumulation in hepatocytes. These mechanisms could, at least partly, explain the adipose tissue TLR5 expression associated with liver fat content in humans. PMID:27035341

  20. Adipocytes as a Link Between Gut Microbiota-Derived Flagellin and Hepatocyte Fat Accumulation

    PubMed Central

    Munukka, Eveliina; Wiklund, Petri; Partanen, Tiina; Välimäki, Sakari; Laakkonen, Eija K.; Lehti, Maarit; Fischer-Posovzsky, Pamela; Wabitsch, Martin; Cheng, Sulin; Huovinen, Pentti; Pekkala, Satu

    2016-01-01

    While the role of both elevated levels of circulating bacterial cell wall components and adipose tissue in hepatic fat accumulation has been recognized, it has not been considered that the bacterial components-recognizing adipose tissue receptors contribute to the hepatic fat content. In this study we found that the expression of adipose tissue bacterial flagellin (FLG)-recognizing Toll-like receptor (TLR) 5 associated with liver fat content (r = 0.699, p = 0.003) and insulin sensitivity (r = -0.529, p = 0.016) in humans (n = 23). No such associations were found for lipopolysaccharides (LPS)-recognizing TLR4. To study the underlying molecular mechanisms of these associations, human HepG2 hepatoma cells were exposed in vitro to the conditioned culture media derived from FLG or LPS-challenged human adipocytes. The adipocyte-mediated effects were also compared to the effects of direct HepG2 exposure to FLG and LPS. We found that the media derived from FLG-treated adipocytes stimulated fat accumulation in HepG2 cells, whereas either media derived from LPS-treated adipocytes or direct FLG or LPS exposure did not. This is likely due to that FLG-treatment of adipocytes increased lipolysis and secretion of glycerol, which is known to serve a substrate for triglyceride synthesis in hepatocytes. Similarly, only FLG-media significantly decreased insulin signaling-related Akt phosphorylation, IRS1 expression and mitochondrial respiratory chain ATP5A. In conclusion, our results suggest that the FLG-induced TLR5 activation in adipocytes increases glycerol secretion from adipocytes and decreases insulin signaling and mitochondrial functions, and increases fat accumulation in hepatocytes. These mechanisms could, at least partly, explain the adipose tissue TLR5 expression associated with liver fat content in humans. PMID:27035341

  1. Constraints on the recent rate of lunar regolith accumulation from Diviner observations

    NASA Astrophysics Data System (ADS)

    Ghent, R. R.; Hayne, P. O.; Bandfield, J. L.; Campbell, B. A.; Carter, L. M.; Allen, C.; Paige, D. A.

    2012-12-01

    Many large craters on the lunar nearside show radar CPR signatures consistent with the presence of blocky ejecta blankets, to distances predicted to be covered by continuous ejecta. However, most of these surfaces show limited enhancements in both derived rock abundance and rock-free regolith temperatures calculated from Diviner nighttime infrared observations. This indicates that the surface rocks are covered by a layer of thermally insulating regolith material. By matching the results of one-dimensional thermal models to Diviner nighttime temperatures, we have constrained the thermophysical properties of the upper regolith, and the thickness of regolith overlying proximal ejecta. We find that for all of the regions surveyed (all in the nearside highlands), the nighttime cooling curves are best fit by a density profile that varies exponentially with depth, consistent with a linear mixture of rocks and regolith fines, with increasing rock content with depth. Our results show significant spatial variations in the density e-folding depth, H, among young crater ejecta regions, indicating differences in the thickness of accumulated regolith. However, away from young craters, the average regional "equilibrium" value of H (Heq) is remarkably consistent, and is on the order of 5 cm. As expected, near-rim ejecta associated with young craters show lower values of H, indicating a high rock content in the shallow subsurface; for older craters, the average value of H approaches the regional value of Heq. Calculated H values for young craters (Giordano Bruno, Moore F, Byrgius A, Necho, Tycho, Jackson, King, and Copernicus) show a clear correlation with published ages, providing the first observational constraint on the recent rate of lunar regolith accumulation. In addition, this result may help to resolve the apparent discrepancy between ages calculated from small crater counts on melt ponds versus counts on continuous ejecta (e.g., King crater; Ashley et al., 2011, LPSC 42

  2. Hydroacoustic and spatial analysis of sediment fluxes and accumulation rates in two Virginia reservoirs, USA.

    PubMed

    Clark, E V; Odhiambo, B K; Yoon, S; Pilati, L

    2015-06-01

    Watershed sediment fluxes and reservoir sediment accumulation rates were analyzed in two contrasting reservoir systems in central and western Virginia. Lake Pelham, located in the Piedmont geologic province, is a human-impacted reservoir with a watershed dominated by agricultural, residential and industrial land uses. Conversely, Lake Moomaw has a largely undeveloped watershed characterized by very steep slopes and forested land use located in the Valley and Ridge province. The Revised Universal Soil Loss Equation (RUSLE) and sediment delivery ratios (SDRs) were used to estimate soil losses in the two watersheds. Bathymetric and sediment accumulation surveys of the two reservoirs were also conducted using a multi-frequency hydroacoustic surveying system. The RUSLE/SDR erosion model estimates 2150 kg ha(-1) year(-1) for Lake Pelham and 2720 kg ha(-1) year(-1) for Lake Moomaw, a 410 and 13 % increase from assumed pristine (100 % forested) land use for the respective basins. Mean sediment accumulation rates of 1.51 and 0.60 cm year(-1) were estimated from the hydroacoustic survey of Lake Pelham and Lake Moomaw, respectively. Overall, Lake Moomaw has relatively low sediment accumulation rates; however, the reservoir is vulnerable to increases in sediment fluxes with further human development due to the steep slopes and highly erodible colluvial soils that characterize the basin. Higher erosion and sediment accumulation rates in Lake Pelham are most likely reflecting the impact of human development on sedimentation processes, where the loss of vegetal buffers and increase in impervious surfaces exacerbates both the surficial soil losses as well as intrinsic stream sediment production leading to the current annual reservoir capacity loss of 0.4 %.

  3. RNA Futile Cycling in Model Persisters Derived from MazF Accumulation

    PubMed Central

    Mok, Wendy W. K.; Park, Junyoung O.; Rabinowitz, Joshua D.

    2015-01-01

    ABSTRACT Metabolism plays an important role in the persister phenotype, as evidenced by the number of strategies that perturb metabolism to sabotage this troublesome subpopulation. However, the absence of techniques to isolate high-purity populations of native persisters has precluded direct measurement of persister metabolism. To address this technical challenge, we studied Escherichia coli populations whose growth had been inhibited by the accumulation of the MazF toxin, which catalyzes RNA cleavage, as a model system for persistence. Using chromosomally integrated, orthogonally inducible promoters to express MazF and its antitoxin MazE, bacterial populations that were almost entirely tolerant to fluoroquinolone and β-lactam antibiotics were obtained upon MazF accumulation, and these were subjected to direct metabolic measurements. While MazF model persisters were nonreplicative, they maintained substantial oxygen and glucose consumption. Metabolomic analysis revealed accumulation of all four ribonucleotide monophosphates (NMPs). These results are consistent with a MazF-catalyzed RNA futile cycle, where the energy derived from catabolism is dissipated through continuous transcription and MazF-mediated RNA degradation. When transcription was inhibited, oxygen consumption and glucose uptake decreased, and nucleotide triphosphates (NTPs) and NTP/NMP ratios increased. Interestingly, the MazF-inhibited cells were sensitive to aminoglycosides, and this sensitivity was blocked by inhibition of transcription. Thus, in MazF model persisters, futile cycles of RNA synthesis and degradation result in both significant metabolic demands and aminoglycoside sensitivity. PMID:26578677

  4. Topographic heterogeneity effect on the accumulation of Fukushima-derived radiocesium on forest floor driven by biologically mediated processes

    NASA Astrophysics Data System (ADS)

    Koarashi, Jun; Atarashi-Andoh, Mariko; Takeuchi, Erina; Nishimura, Syusaku

    2014-10-01

    The accident at the Fukushima Daiichi nuclear power plant caused serious radiocesium (137Cs) contamination of forest ecosystems located in mountainous and hilly regions with steep terrain. To understand topographic effects on the redistribution and accumulation of 137Cs on forest floor, we investigated the distribution of Fukushima-derived 137Cs in forest-floor litter layers on a steep hillslope in a Japanese deciduous forest in August 2013 (29 months after the accident). Both leaf-litter materials and litter-associated 137Cs were accumulated in large amounts at the bottom of the hillslope. At the bottom, a significant fraction (65%) of the 137Cs inventory was observed to be associated with newly shed and less degraded leaf-litter materials, with estimated mean ages of 0.5-1.5 years, added via litterfall after the accident. Newly emerged leaves were contaminated with Fukushima-derived 137Cs in May 2011 (two months after the accident) and 137Cs concentration in them decreased with time. However, the concentrations were still two orders of magnitude higher than the pre-accident level in 2013 and 2014. These observations are the first to show that 137Cs redistribution on a forested hillslope is strongly controlled by biologically mediated processes and continues to supply 137Cs to the bottom via litterfall at a reduced rate.

  5. Topographic heterogeneity effect on the accumulation of Fukushima-derived radiocesium on forest floor driven by biologically mediated processes.

    PubMed

    Koarashi, Jun; Atarashi-Andoh, Mariko; Takeuchi, Erina; Nishimura, Syusaku

    2014-01-01

    The accident at the Fukushima Daiichi nuclear power plant caused serious radiocesium ((137)Cs) contamination of forest ecosystems located in mountainous and hilly regions with steep terrain. To understand topographic effects on the redistribution and accumulation of (137)Cs on forest floor, we investigated the distribution of Fukushima-derived (137)Cs in forest-floor litter layers on a steep hillslope in a Japanese deciduous forest in August 2013 (29 months after the accident). Both leaf-litter materials and litter-associated (137)Cs were accumulated in large amounts at the bottom of the hillslope. At the bottom, a significant fraction (65%) of the (137)Cs inventory was observed to be associated with newly shed and less degraded leaf-litter materials, with estimated mean ages of 0.5-1.5 years, added via litterfall after the accident. Newly emerged leaves were contaminated with Fukushima-derived (137)Cs in May 2011 (two months after the accident) and (137)Cs concentration in them decreased with time. However, the concentrations were still two orders of magnitude higher than the pre-accident level in 2013 and 2014. These observations are the first to show that (137)Cs redistribution on a forested hillslope is strongly controlled by biologically mediated processes and continues to supply (137)Cs to the bottom via litterfall at a reduced rate.

  6. Topographic heterogeneity effect on the accumulation of Fukushima-derived radiocesium on forest floor driven by biologically mediated processes.

    PubMed

    Koarashi, Jun; Atarashi-Andoh, Mariko; Takeuchi, Erina; Nishimura, Syusaku

    2014-01-01

    The accident at the Fukushima Daiichi nuclear power plant caused serious radiocesium ((137)Cs) contamination of forest ecosystems located in mountainous and hilly regions with steep terrain. To understand topographic effects on the redistribution and accumulation of (137)Cs on forest floor, we investigated the distribution of Fukushima-derived (137)Cs in forest-floor litter layers on a steep hillslope in a Japanese deciduous forest in August 2013 (29 months after the accident). Both leaf-litter materials and litter-associated (137)Cs were accumulated in large amounts at the bottom of the hillslope. At the bottom, a significant fraction (65%) of the (137)Cs inventory was observed to be associated with newly shed and less degraded leaf-litter materials, with estimated mean ages of 0.5-1.5 years, added via litterfall after the accident. Newly emerged leaves were contaminated with Fukushima-derived (137)Cs in May 2011 (two months after the accident) and (137)Cs concentration in them decreased with time. However, the concentrations were still two orders of magnitude higher than the pre-accident level in 2013 and 2014. These observations are the first to show that (137)Cs redistribution on a forested hillslope is strongly controlled by biologically mediated processes and continues to supply (137)Cs to the bottom via litterfall at a reduced rate. PMID:25358420

  7. Formation and distribution of coal measure-derived hydrocarbon accumulation in NW China

    SciTech Connect

    Wenxhi Zhao; Yan Zhang; Dafeng Xu; Changyi Zhao )

    1996-01-01

    This study recognizes that the following conditions are necessary for the Jurassic coal measure-derived oil and gas fields in NW China: (1) The Jurassic original basins, which were dominated by lacustrine to low-positional swamp environments, should exist to accept coal-measure related sediments, (2) the original depositional settings were characterized by low and gentle depressions and humid climate where oil-prone organic materials accumulated, (3) the development of the subsequent basins and successive sedimentation should occur on the Jurassic original basins, which are necessary to keep the coal measure source rocks progressively maturing, and, (4) a certain degree of tectonic compression took place soon after the maturation of source rocks, which provided the driving force for the effective expulsion of oil and gas from coal measures. Most of the coal measure-derived oil gas fields in NW China are horizontally distributed along the inner side of lake strandlines. They occur vertically above or below the threshold of maturation. Owing to the sharp variations of lithology and facies in coal measures, the lithologies and hydrocarbon compositions of oil and gas reservoirs play a significant role in the oil and gas accumulations of the coal measures sequences.

  8. Formation and distribution of coal measure-derived hydrocarbon accumulation in NW China

    SciTech Connect

    Wenxhi Zhao; Yan Zhang; Dafeng Xu; Changyi Zhao

    1996-12-31

    This study recognizes that the following conditions are necessary for the Jurassic coal measure-derived oil and gas fields in NW China: (1) The Jurassic original basins, which were dominated by lacustrine to low-positional swamp environments, should exist to accept coal-measure related sediments, (2) the original depositional settings were characterized by low and gentle depressions and humid climate where oil-prone organic materials accumulated, (3) the development of the subsequent basins and successive sedimentation should occur on the Jurassic original basins, which are necessary to keep the coal measure source rocks progressively maturing, and, (4) a certain degree of tectonic compression took place soon after the maturation of source rocks, which provided the driving force for the effective expulsion of oil and gas from coal measures. Most of the coal measure-derived oil & gas fields in NW China are horizontally distributed along the inner side of lake strandlines. They occur vertically above or below the threshold of maturation. Owing to the sharp variations of lithology and facies in coal measures, the lithologies and hydrocarbon compositions of oil and gas reservoirs play a significant role in the oil and gas accumulations of the coal measures sequences.

  9. Soil carbon stocks and their rates of accumulation and loss in a boreal forest landscape

    USGS Publications Warehouse

    Rapalee, G.; Trumbore, S.E.; Davidson, E.A.; Harden, J.W.; Veldhuis, H.

    1998-01-01

    Boreal forests and wetlands are thought to be significant carbon sinks, and they could become net C sources as the Earth warms. Most of the C of boreal forest ecosystems is stored in the moss layer and in the soil. The objective of this study was to estimate soil C stocks (including moss layers) and rates of accumulation and loss for a 733 km2 area of the BOReal Ecosystem-Atmosphere Study site in northern Manitoba, using data from smaller-scale intensive field studies. A simple process-based model developed from measurements of soil C inventories and radiocarbon was used to relate soil C storage and dynamics to soil drainage and forest stand age. Soil C stocks covary with soil drainage class, with the largest C stocks occurring in poorly drained sites. Estimated rates of soil C accumulation or loss are sensitive to the estimated decomposition constants for the large pool of deep soil C, and improved understanding of deep soil C decomposition is needed. While the upper moss layers regrow and accumulate C after fires, the deep C dynamics vary across the landscape, from a small net sink to a significant source. Estimated net soil C accumulation, averaged for the entire 733 km2 area, was 20 g C m-2 yr-1 (28 g C m-2 yr-1 accumulation in surface mosses offset by 8 g C m-2 yr-1 lost from deep C pools) in a year with no fire. Most of the C accumulated in poorly and very poorly drained soils (peatlands and wetlands). Burning of the moss layer in only 1% of uplands would offset the C stored in the remaining 99% of the area. Significant interannual variability in C storage is expected because of the irregular occurrence of fire in space and time. The effects of climate change and management on fire frequency and on decomposition of immense deep soil C stocks are key to understanding future C budgets in boreal forests.

  10. Comparison of Current and Historical Rates of Ecosystem Carbon Accumulation in a Northern Alberta Peatland

    NASA Astrophysics Data System (ADS)

    Syed, K. H.; Flanagan, L. B.; Carlson, P. J.; Glenn, A. J.; Ponton, S.

    2005-12-01

    As part of Fluxnet-Canada, we have been investigating the environmental controls on net ecosystem carbon dioxide exchange using the eddy covariance technique in a moderately rich (treed) fen in northern Alberta, Canada. In addition, integrated CO2 fluxes were compared to carbon stock measurements and rates of peat accumulation. The total ecosystem carbon stock was 52,669 g C m-2 with the vast majority (52,129) accumulated in peat over a 2 meter depth. The basal age for the peat was 2210 ± 50 years before present. The above-ground carbon stock in the two tree species was 226 g C m-2. The oldest Picea mariana trees were aged at 135 years, and they showed a rapid increase in basal area increment starting about 65 years ago that peaked at rates of 2 cm2 yr-1 about 40 years ago. The Larix laricina trees became established approximately 45 years ago and currently have a basal area increment of 3 to 4 cm2 yr-1, much higher than the current rates (0.5 cm2 yr-1) observed for Picea mariana. The rates of peat accumulation were determined on 210Pb-dated cores. Over the last 70 years the peat gained an average of 113 ± 12 g C m-2 yr-1. This was similar to net ecosystem production measured by eddy covariance (95 and 210 g C m-2 yr-1) over the last two years. Variation in annual net ecosystem production was associated with shifts in weather and growing season length. Current and recent historical rates of carbon accumulation were quite consistent despite significant variation in tree species growth and successional changes in this peatland over the last 70 years.

  11. The distribution of the heavy metal accumulation rate in the biomass of three Daphnia species

    SciTech Connect

    Gajula, V.K.; Hovorka, J.; Stuchlik, E.

    1995-12-31

    The difference in the accumulation rate of a mixture of heavy metals in aquatic organisms is of considerable interest because of its importance in the prediction of the effect of pollutants in aquatic systems. In this study the authors are making an effort to evaluate the accumulation patterns of pollutants in aquatic organisms by establishing a relation between the level of an accumulated mixture of heavy metals (Cd, Zn, Pb, As, Hg) in individuals of Daphnia magna, Daphnia pulicaria and Daphnia galeata and its dry weight with respect to the form of heavy metals in the aquatic environment. One age group of Daphnia species (10 day old) were exposed to 5 ppb, 10 ppb and 20 ppb of the mixture of heavy metals for 24 hours in three different experiments. In the first experiment the mixture of heavy metals was present exclusively in labelled algae (Scendesmus actus), in the second in an aquatic medium with non labelled algae, and in the third experiment the mixture of heavy metals was dissolved in the aquatic medium only without the addition of algae. The concentration of the heavy metal mixture in individuals of D.magna; D.pulicaria and D.galeata was determined using atomic absorption spectrometry. Results were statistically evaluated and the rate of accumulation and influence of various heavy metals in the biomass of three Daphnia species is discussed.

  12. Trawling-induced alterations of deep-sea sediment accumulation rates during the Anthropocene

    NASA Astrophysics Data System (ADS)

    Puig, P.; Paradis, S.; Masque, P.; Martin, J.; Juan, X.; Palanques, A.

    2015-12-01

    Commercial bottom trawling causes direct physical disturbance of the marine sedimentary environments by scraping and ploughing the seabed, generating periodic resuspension of surface sediments. However, the quantification of the sediment that is removed by trawling and exported across the continental margin remains largely unaddressed, and the preservation of the signal of such impacts in the geological record have been mostly overlooked. The analysis of sediment cores collected along the Catalan margin (NW Mediterranean) has allowed evaluating the contribution of this anthropogenic activity to the present-day sediment dynamics. Sediment cores at intensively trawled sites are characterized by over-consolidated sediments with lower 210Pb surface concentrations and inventories that indicate widespread erosion of recent sedimentary deposits. In turn, combined 210Pb and 137Cs chronologies indicate a significant increase of sediment accumulation rates within submarine canyon environments since the 1970s, coincidently with a strong impulse in the industrialization of the trawling fleets of this region. Two sampling sites that exhibited high sediment accumulation rates (0.6-0.7 cm/y) were reoccupied 1-2 decades after the first studies and revealed a second and even larger increase of sediment accumulation rates (>2 cm/y) occurring at the beginning of the XXI century. This recent change has been attributed to a preferential displacement of the trawling fleet towards fishing grounds surrounding submarine canyons and, also, to technical improvements in trawling vessels, presumably related to financial subsidies provided to the fishing sector. The alteration of sediment accumulation rates described in this continental margin may occur in many regions of the World's oceans given the wide geographical distribution of this human activity, and therefore, it could represent a potential marker of the Anthropocene in deep-sea environments.

  13. Organic Carbon, Nitrogen and Phosphorus Accumulation Rates in the Soils of the Everglades Mangrove Ecotone

    NASA Astrophysics Data System (ADS)

    Smoak, J. M.; Breithaupt, J. L.; Sanders, C. J.

    2015-12-01

    One of the fundamental questions with regard to coastal ecotones relates to their role in the transformation, transport and storage of biogeochemically important constituents and how that role may be altered by climate change. Coastal wetlands provide a range of valuable ecosystem services including sequestering organic carbon (OC) and nutrients in their soils at rates greater than terrestrial ecosystems on a per area basis. As such the Everglades mangrove ecotone, the largest contiguous mangrove forest in North America, is a biogeochemical "hotspot" at the interface of freshwater marsh and the Gulf of Mexico. Over the last one hundred years this region has been impacted by a reduction in freshwater flow and a sea-level rise (SLR) of 2.3 mm/yr which combined to cause a landward shift in the ecotone. This creates an ideal setting to examine climate induced alterations in the mangrove-ecotone biogeochemical cycle. The ability of the Everglades mangrove forest to keep pace with SLR depends largely on the rate of organic matter accumulation as that accumulation is a key contributor to accretion. However, the basic threat from SLR can be exacerbated in some areas by accelerating organic matter mineralization due to increasing salinity. The increase in salinity supplies sulfate which functions as a terminal electron acceptor that soil microbes can utilize to enhance mineralization in the brackish ecotone regions of coastal wetlands. To investigate these processes, we measured mangrove forest soil accretion, OC, N and P accumulation rates over the most recent 10, 50 and 100 year periods (via 210Pb dating) from the Gulf of Mexico to the upper freshwater reaches of the mangrove forest within Everglades National Park. Lower organic carbon accumulation rates compared to the rest of the system were found in the ecotone region most susceptible to enhanced organic matter mineralization.

  14. Balancing sample accumulation and DNA degradation rates to optimize noninvasive genetic sampling of sympatric carnivores.

    PubMed

    Lonsinger, Robert C; Gese, Eric M; Dempsey, Steven J; Kluever, Bryan M; Johnson, Timothy R; Waits, Lisette P

    2015-07-01

    Noninvasive genetic sampling, or noninvasive DNA sampling (NDS), can be an effective monitoring approach for elusive, wide-ranging species at low densities. However, few studies have attempted to maximize sampling efficiency. We present a model for combining sample accumulation and DNA degradation to identify the most efficient (i.e. minimal cost per successful sample) NDS temporal design for capture-recapture analyses. We use scat accumulation and faecal DNA degradation rates for two sympatric carnivores, kit fox (Vulpes macrotis) and coyote (Canis latrans) across two seasons (summer and winter) in Utah, USA, to demonstrate implementation of this approach. We estimated scat accumulation rates by clearing and surveying transects for scats. We evaluated mitochondrial (mtDNA) and nuclear (nDNA) DNA amplification success for faecal DNA samples under natural field conditions for 20 fresh scats/species/season from <1-112 days. Mean accumulation rates were nearly three times greater for coyotes (0.076 scats/km/day) than foxes (0.029 scats/km/day) across seasons. Across species and seasons, mtDNA amplification success was ≥95% through day 21. Fox nDNA amplification success was ≥70% through day 21 across seasons. Coyote nDNA success was ≥70% through day 21 in winter, but declined to <50% by day 7 in summer. We identified a common temporal sampling frame of approximately 14 days that allowed species to be monitored simultaneously, further reducing time, survey effort and costs. Our results suggest that when conducting repeated surveys for capture-recapture analyses, overall cost-efficiency for NDS may be improved with a temporal design that balances field and laboratory costs along with deposition and degradation rates.

  15. Balancing sample accumulation and DNA degradation rates to optimize noninvasive genetic sampling of sympatric carnivores.

    PubMed

    Lonsinger, Robert C; Gese, Eric M; Dempsey, Steven J; Kluever, Bryan M; Johnson, Timothy R; Waits, Lisette P

    2015-07-01

    Noninvasive genetic sampling, or noninvasive DNA sampling (NDS), can be an effective monitoring approach for elusive, wide-ranging species at low densities. However, few studies have attempted to maximize sampling efficiency. We present a model for combining sample accumulation and DNA degradation to identify the most efficient (i.e. minimal cost per successful sample) NDS temporal design for capture-recapture analyses. We use scat accumulation and faecal DNA degradation rates for two sympatric carnivores, kit fox (Vulpes macrotis) and coyote (Canis latrans) across two seasons (summer and winter) in Utah, USA, to demonstrate implementation of this approach. We estimated scat accumulation rates by clearing and surveying transects for scats. We evaluated mitochondrial (mtDNA) and nuclear (nDNA) DNA amplification success for faecal DNA samples under natural field conditions for 20 fresh scats/species/season from <1-112 days. Mean accumulation rates were nearly three times greater for coyotes (0.076 scats/km/day) than foxes (0.029 scats/km/day) across seasons. Across species and seasons, mtDNA amplification success was ≥95% through day 21. Fox nDNA amplification success was ≥70% through day 21 across seasons. Coyote nDNA success was ≥70% through day 21 in winter, but declined to <50% by day 7 in summer. We identified a common temporal sampling frame of approximately 14 days that allowed species to be monitored simultaneously, further reducing time, survey effort and costs. Our results suggest that when conducting repeated surveys for capture-recapture analyses, overall cost-efficiency for NDS may be improved with a temporal design that balances field and laboratory costs along with deposition and degradation rates. PMID:25454561

  16. The amount and accumulation rate of plastic debris on marshes and beaches on the Georgia coast.

    PubMed

    Lee, Richard F; Sanders, Dorothea P

    2015-02-15

    The amount and accumulation rate of plastic debris at 20 sites along the Georgia coast were prepared using data reported by a number of volunteer organizations. The amount of plastic debris at highly visited barrier island beaches and estuarine marshes ranged from 300 to >1000 kg. Relatively large amount of plastics (180-500 kg) were found on less visited barrier island beaches, i.e. Blackbeard, Ossabaw and Cumberland Islands. A follow up monthly or quarterly collection study was carried out on two of the sites, a barrier beach and estuarine marsh, to determine accumulation rate in 8000 m(2) areas. Accumulation rates ranged from 0.18 to 1.28 kg/30 days-8000 m(2) on the barrier island beach and from 0.6 to 1.61 kg/30 days-8000 m(2) at the estuarine marsh site. The major type of plastics, e.g. bottles, food wrappers, plastic fragments, was highly variable at different seasons and sites. The authors recommend consideration of a standardization in reporting plastic debris, with respect to quantitation of debris and sample area.

  17. Accumulation rates of Th-230, Pa-231, and some transition metals on the Bermuda Rise

    USGS Publications Warehouse

    Bacon, M.P.; Rosholt, J.N.

    1982-01-01

    Measurements of 238U, 234U, 230Th, 232Th, 231Pa, Mn, Fe, Co, Ni, Cu, and Zn were made on 23 samples from core GPC-5, a 29-m giant piston core from a water depth of 4583 m on the northeastern Bermuda Rise (33??41.2???N, 57??36.9???W). This area is characterized by rapid deposition of sediment transported by abyssal currents. Unsupported 230Th and 231Pa are present throughout the core but, because of large variations in the sedimentation rate, show marked departures from exponential decay with depth. The trend with depth of the 231Paex 230Thex ratio is consistent with the average accumulation rate of 36 cm/1000 y reported earlier on the basis of radiocarbon dating and CaCO3 stratigraphy. When expressed on a carbonate-free basis, concentrations of Mn, Co, Ni, Cu, Zn, 230Thex, and 231Paex all show cyclic variations positively correlated with those of CaCO3. The correlations can be explained by a model in which all of these constituents, including CaCO3, are supplied to the sediments from the water column at a constant rate. Concentration variations are controlled mainly by varying inputs of terrigenous detritus, with low inputs occurring during interglacials and high inputs during glacials. Relationships between the metal and 230Thex concentrations permit estimates of the rates at which the metals are removed to the sediment by scavenging from the water column. The results, in ??g/cm2-1000 y, are: 4300 ?? 1100 for Mn, 46 ?? 16 for Ni and 76 ?? 26 for Cu. These rates are somewhat larger than ocean-wide averages estimated by other methods, and the absolute rate of 230Th accumulation in GPC-5 averages about nine times higher than production in the overlying water column. This part of the Bermuda Rise and similar bottom-current deposits may act as important accumulators of elements scavenged from seawater. ?? 1982.

  18. Accumulation rate and mixing of shelf sediments in the Monterey Bay National Marine Sanctuary

    USGS Publications Warehouse

    Lewis, R.C.; Coale, K.H.; Edwards, B.D.; Marot, M.; Douglas, J.N.; Burton, E.J.

    2002-01-01

    The distribution of excess 210Pb in 31 sediment cores was used to determine modern (last 100 yr) mass accumulation rates and the depth of sediment mixing on the continental shelf between Pacifica and Monterey, California, USA. Apparent mass accumulation rates average 0.27 g cm-2 yr-1 and range from 0.42 g cm-2 yr-1 to 0.12 g cm-2 yr-1. Accumulation rates were highest at mid-shelf water depths (60-100 m) adjacent to major rivers and near the head of the Ascension submarine canyon. Cores from water depths of less than 65 m had low, uniform 210Pb activity profiles and sandy textures. The uppermost 5-13 cm of 15 cores had uniform 210Pb activity profiles above a region of steadily decreasing 210Pb activity. This phenomenon was attributed to sediment mixing. The thickness of this upper layer of uniform 210Pb activity decreased southward from 13 cm, west of Pacifica, to less than 5 cm, near Monterey Canyon. This southward decrease may be attributed to shallower bioturbation in the southern study area. Integrated excess 210Pb activities were generally higher where sedimentation rates were high. They were also higher with increasing distance from major rivers. Thus, sedimentation rate alone does not explain the distribution of integrated excess 210Pb in this study area. Excess 210Pb in the seafloor is controlled by other factors such as sediment texture, the atmospheric deposition rate of 210Pb, and the residence time of sediment particles in the water column. ?? 2002 Elsevier Science B.V. All rights reserved.

  19. Derivation of instanton rate theory from first principles

    NASA Astrophysics Data System (ADS)

    Richardson, Jeremy O.

    2016-03-01

    Instanton rate theory is used to study tunneling events in a wide range of systems including low-temperature chemical reactions. Despite many successful applications, the method has never been obtained from first principles, relying instead on the "Im F" premise. In this paper, the same expression for the rate of barrier penetration at finite temperature is rederived from quantum scattering theory [W. H. Miller, S. D. Schwartz, and J. W. Tromp, J. Chem. Phys. 79, 4889 (1983)] using a semiclassical Green's function formalism. This justifies the instanton approach and provides a route to deriving the rate of other processes.

  20. Modern rates of glacial sediment accumulation along a 15° S-N transect in fjords from the Antarctic Peninsula to southern Chile

    NASA Astrophysics Data System (ADS)

    Boldt, Katherine V.; Nittrouer, Charles A.; Hallet, Bernard; Koppes, Michele N.; Forrest, Brittany K.; Wellner, Julia S.; Anderson, John B.

    2013-12-01

    of glacial erosion in temperate climates rank among the highest worldwide, and the sedimentary products of such erosion record climatic and tectonic signals in many glaciated settings, as well as temporal changes in glacier behavior. Glacial sediment yields are expected to decrease with increasing latitude because decreased temperature and meltwater production reduce glacial sliding, erosion, and sediment transfer; however, this expectation lacks a solid supportive database. Herein we present modern 210Pb-derived sediment accumulation rates on decadal to century time scales for 12 fjords spanning 15° of latitude from the Antarctic Peninsula to southern Chile and interpret the results in light of glacimarine sediment accumulation worldwide. 210Pb records from the Antarctic Peninsula show surprisingly steady sediment accumulation throughout the past century at rates of 1-7 mm yr-1, despite rapid warming and glacial retreat. Cores from the South Shetland Islands reveal accelerated sediment accumulation over the past few decades, likely due to changes in the thermal state of the glaciers in this region, which straddles the boundary between subpolar and temperate conditions. In Patagonia and Tierra del Fuego, sediment accumulates faster (11-24 mm yr-1), and previously collected seismic profiles show that rates reach meters per year close to the glacier termini. This increase in sediment accumulation rates with decreasing latitude reflects the gradient from subpolar to temperate climates and is consistent with glacial erosion being much faster in the temperate climate of southern Chile than in the polar climate of the Antarctic Peninsula.

  1. The forest floor characteristics and C accumulation rate of black spruce forests across a climate gradient

    NASA Astrophysics Data System (ADS)

    Vogel, J. G.; Schuur, E. A. G.

    2014-12-01

    In the North American boreal forest, black spruce forests accumulate a C pool in the FF that is often greater than the vegetation C pool. In addition, the FF's insulating properties help maintain colder soil temperatures and inhibit the microbial decomposition of 'deep' soil C. To estimate how climate change might alter the FF of black spruce forests, we examined the C accumulation rate and characteristics (percent wood, fine roots, moss, and needles; and bulk density) of the FF for three stands in four climatic regions of Alaska. Mean annual temperature ranged from -2.9 °C to 2.3 °C and precipitation from 263 mm to 716 mm and the two climatic variables correlated with one another. Both climatic factors were unrelated with the total C stored in the FF or its rate of C accumulation, despite trees growing faster with warmer and wetter average conditions. Although FF mass was similar across regions, its thickness decreased with temperature and precipitation, reflecting a significant increase in bulk density. The change in bulk density could have been from a shift in constituents because the warmer and wetter regions accumulated more wood and less fine root material. The shift between these two constituents may have also been the reason that the FF mass remained unchanged across the climate gradient because wood has a decomposition rate that is ~10% of fine roots. Although these results suggest that there will be little change in FF mass under warmer and wetter conditions, the increase in FF bulk density will amplify the warming of the mineral soil because of the increase in FF thermal conductivity.

  2. Winter Insulation By Snow Accumulation in a Subarctic Treeline Ecosystem Increases Summer Carbon Cycling Rates

    NASA Astrophysics Data System (ADS)

    Parker, T.; Subke, J. A.; Wookey, P. A.

    2014-12-01

    The effect of snow accumulation on soil carbon and nutrient cycling is attracting substantial attention from researchers. We know that deeper snow accumulation caused by high stature vegetation increases winter microbial activity and therefore carbon and nitrogen flux rates. However, until now the effect of snow accumulation, by buffering winter soil temperature, on subsequent summer soil processes, has scarcely been considered. We carried out an experiment at an alpine treeline in subarctic Sweden in which soil monoliths, contained within PVC collars, were transplanted between forest (deep winter snow) and tundra heath (shallow winter snow). We measured soil CO2efflux over two growing seasons and quantified soil microbial biomass after the second winter. We showed that respiration rates of transplanted forest soil were significantly reduced compared with control collars (remaining in the forest) as a consequence of colder, but more variable, winter temperatures. We hypothesised that microbial biomass would be reduced in transplanted forests soils but found there was no difference compared to control. We therefore further hypothesised that the similarly sized microbial pool in the control is assembled differently to the transplant. We believe that the warmer winters in forests foster more active consortia of decomposer microbes as a result of different abiotic selection pressures. Using an ecosystem scale experimental approach, we have identified a mechanism that influences summer carbon cycling rates based solely on the amount of snow that accumulates the previous winter. We conclude that modification of snow depth as a consequence of changes in vegetation structure is an important mechanism influencing soil C stocks in ecosystems where snow persists for a major fraction of the year.

  3. Calibrating a long-term meteoric 10Be accumulation rate in soil

    NASA Astrophysics Data System (ADS)

    Reusser, Lucas; Graly, Joseph; Bierman, Paul; Rood, Dylan

    2010-10-01

    Using 13 samples collected from a 4.1 meter profile in a well-dated and stable New Zealand fluvial terrace, we present the first long-term accumulation rate for meteoric 10Be in soil (1.68 to 1.72 × 106 at/(cm2·yr)) integrated over the past ˜18 ka. Site-specific accumulation data, such as these, are prerequisite to the application of meteoric 10Be in surface process studies. Our data begin the process of calibrating long-term meteoric 10Be delivery rates across latitude and precipitation gradients. Our integrated rate is lower than contemporary meteoric 10Be fluxes measured in New Zealand rainfall, suggesting that long-term average precipitation, dust flux, or both, at this site were less than modern values. With accurately calibrated long-term delivery rates, such as this, meteoric 10Be will be a powerful tool for studying rates of landscape change in environments where other cosmogenic nuclides, such as in situ 10Be, cannot be used.

  4. Sediment mixing and accumulation rate effects on radionuclide depth profiles in Hudson estuary sediments

    SciTech Connect

    Olsen, C.R.; Simpson, H.J.; Peng, T.H.; Bopp, R.F.; Trier, R.M.

    1981-11-01

    Measured anthropogenic radionuclide profiles in sediment cores from the Hudson River estuary were compared with profiles computed by using known input histories of radionuclides to the estuary and mixing coefficients which decreased exponentially with depth in the sediment. Observed /sup 134/Cs sediment depth profiles were used in the mixing rate computation because reactor releases were the only significant source for this nuclide, whereas the inputs of /sup 137/Cs and /sup 239,240/Pu to the estuary were complicated by runoff or erosion in upstream areas, in addition to direct fallout from precipitation. Our estimates for the rates of surface sediment mixing in the low salinity reach of the estuary range from 0.25 to 1 cm/sup 2//yr, or less. In some areas of the harbor adjacent to New York City, where fine-particle accumulation rates are generally 3 cm/yr, and often as high as 10 to 20 cm/yr, sediment mixing rates as high as 10 cm/sup 2//yr would have little effect on radionuclide peak distributions. Consequently, anthropogenic radionuclide maximum activities in subsurface sediments of the Hudson appear to be useful as time-stratigraphic reference levels, which can be correlated with periods of maximum radionuclide inputs for estimating rates and patterns of sediment accumulation. 10 figures.

  5. Sediment mixing and accumulation rate effects on radionuclide depth profiles in Hudson estuary sediments

    SciTech Connect

    Olsen, C.R.; Simpson, H.J.; Peng, T.; Bopp, R.F.; Trier, R.M.

    1981-11-20

    Measured anthropogenic radionuclide profiles in sediment cores from the Hudson River estuary were compared with profiles computed by using known input histories of radionuclides to the estuary and mixing coefficients which decreased exponentially with depth in the sediment. Observed /sup 134/Cs sediment depth profiles were used in the mixing rate computation because reactor releases were the only significant source for this nuclide, whereas the inputs of /sup 137/Cs and /sup 239.240/Pu to the estuary were complicated by runoff or erosion in upstream areas, in addition to direct fallout from precipitation. Our estimates for the rates of surface sediment mixing in the low salinity reach of the estuary range from 0.25 to 1 cm/sup 2//yr, or less. In some areas of the harbor adjacent to New York City, were fine-particle accumulation rates are generally >3 cm/yr, and often as high as 10 to 20 cm/yr, sediment mixing rates as high as 10 cm/sup 2//yr would have little effect on radionuclide peak distributions. Consequently, anthropogenic radionuclide maximum activities in subsurface sediments of the Hudson appear to be useful as time-stratigraphic reference levels, which can be correlated with periods of maximum radionuclide inputs for estimating rates and patterns of sediment accumulation.

  6. Tumor-derived IL-35 promotes tumor growth by enhancing myeloid cell accumulation and angiogenesis.

    PubMed

    Wang, Zhihui; Liu, Jin-Qing; Liu, Zhenzhen; Shen, Rulong; Zhang, Guoqiang; Xu, Jianping; Basu, Sujit; Feng, Youmei; Bai, Xue-Feng

    2013-03-01

    IL-35 is a member of the IL-12 family of cytokines that is comprised of an IL-12 p35 subunit and an IL-12 p40-related protein subunit, EBV-induced gene 3 (EBI3). IL-35 functions through IL-35R and has a potent immune-suppressive activity. Although IL-35 was demonstrated to be produced by regulatory T cells, gene-expression analysis revealed that it is likely to have a wider distribution, including expression in cancer cells. In this study, we demonstrated that IL-35 is produced in human cancer tissues, such as large B cell lymphoma, nasopharyngeal carcinoma, and melanoma. To determine the roles of tumor-derived IL-35 in tumorigenesis and tumor immunity, we generated IL-35-producing plasmacytoma J558 and B16 melanoma cells and observed that the expression of IL-35 in cancer cells does not affect their growth and survival in vitro, but it stimulates tumorigenesis in both immune-competent and Rag1/2-deficient mice. Tumor-derived IL-35 increases CD11b(+)Gr1(+) myeloid cell accumulation in the tumor microenvironment and, thereby, promotes tumor angiogenesis. In immune-competent mice, spontaneous CTL responses to tumors are diminished. IL-35 does not directly inhibit tumor Ag-specific CD8(+) T cell activation, differentiation, and effector functions. However, IL-35-treated cancer cells had increased expression of gp130 and reduced sensitivity to CTL destruction. Thus, our study indicates novel functions for IL-35 in promoting tumor growth via the enhancement of myeloid cell accumulation, tumor angiogenesis, and suppression of tumor immunity.

  7. Carbon accumulation rate of peatland in the High Arctic, Svalbard: Implications for carbon sequestration

    NASA Astrophysics Data System (ADS)

    Nakatsubo, Takayuki; Uchida, Masaki; Sasaki, Akiko; Kondo, Miyuki; Yoshitake, Shinpei; Kanda, Hiroshi

    2015-06-01

    Moss tundra that accumulates a thick peat layer is one of the most important ecosystems in the High Arctic, Svalbard. The importance of this ecosystem for carbon sequestration was estimated from the apparent rates of carbon accumulation based on the 14C age and amount of peat in the active layer. The study site at Stuphallet, Brøgger Peninsula, northwestern Svalbard was covered with a thick peat layer dominated by moss species such as Calliergon richardsonii, Paludella squarrosa, Tomenthypnum nitens, and Warnstorfia exannulata. The average thickness of the active layer (brown moss and peat) was approximately 28 cm in 1 August 2011. The calibrated (cal) age of peat from the bottom of the active layer (20-30 cm below the peatland surface) ranged from 81 to 701 cal yr BP (median value of 2σ range). Based on the total carbon (4.5-9.2 kg C m-2), the apparent rate of carbon accumulation in the active layer was 9.0-19.2 (g C m-2 yr-1), which is similar to or greater than the net ecosystem production or net primary production reported for other vegetation types in this area. Our data suggest that moss tundra plays an important role in carbon sequestration in this area.

  8. Relationship between the cellular accumulation and the cytotoxicity of S12363, a new Vinca alkaloid derivative.

    PubMed

    Pierré, A; Pérez, V; Léonce, S; Boutin, J A; Saint-Dizier, D; Hautefaye, P; Lavielle, G; Atassi, G

    1992-01-01

    S12363, a new vinca alkaloid derivative, was considerably more cytotoxic to murine L1210 cells and five human tumor cell lines (HL60, HT-29, COLO 320DM, NCI-H460, and PANC-1) than was vincristine (VCR) or vinblastine (VLB). S 12,363 bound to tubulin in crude extracts from brain or L1210 cells with an affinity similar to that of VLB and VCR (apparent Kd value: 1.1-1.6, 1.2-1.7, and 0.6-0.8 microM, respectively). After 1 h exposure, the accumulation of 20 nM [3H]-S 12,363 by L1210 cells was 4- to 18-fold that of [3H]-VLB and [3H]-VCR, respectively. After the cells had been preloaded for 1 h with the labeled drugs and then incubated for 3 h in drug-free medium, 37%-55% of the [3H]-S 12,363 was retained by the cells vs 36%-47% of the [3H]-VCR and less than 6% of the [3H]-VLB. Similar results were obtained for the five human cell lines tested. The accumulation factors (intracellular vs extracellular concentrations) found for [3H]-S 12,363 (54- to 167-fold) were significantly higher than those observed for [3H]-VCR (5- to 14-fold) or [3H]-VLB (19- to 41-fold). Greater than 90% of the radioactivity extracted from L1210 cells that had been treated with [3H]-S 12,363 was recovered as unmodified drug, demonstrating that [3H]-S 12,363 was not metabolized by these cells. S 12,362, which differs from S 12,363 only in the absolute configuration of the asymmetric carbon atom of its alpha-aminophosphonic side chain, was 300 times less cytotoxic, bound to tubulin with a lower affinity (apparent Kd value, 4.9-9.6 microM), and was neither accumulated nor retained by the cells. Taken together, these results demonstrate that the potency of S 12,363 is due at least in part to its cellular accumulation and retention.

  9. Systems Level Regulation of Rhythmic Growth Rate and Biomass Accumulation in Grasses

    SciTech Connect

    Kay, Steve A.

    2013-05-02

    Several breakthroughs have been recently made in our understanding of plant growth and biomass accumulation. It was found that plant growth is rhythmically controlled throughout the day by the circadian clock through a complex interplay of light and phytohormone signaling pathways. While plants such as the C4 energy crop sorghum (Sorghum bicolor (L.) Moench) and possibly the C3 grass (Brachypodium distachyon) also exhibit daily rhythms in growth rate, the molecular details of its regulation remain to be explored. A better understanding of diurnally regulated growth behavior in grasses may lead to species-specific mechanisms highly relevant to future strategies to optimize energy crop biomass yield. Here we propose to devise a systems approach to identify, in parallel, regulatory hubs associated with rhythmic growth in C3 and C4 plants. We propose to use rhythmicity in daily growth patterns to drive the discovery of regulatory network modules controlling biomass accumulation.

  10. LATE CENOZOIC INCREASE IN ACCUMULATION RATES OF TERRESTRIAL SEDIMENT: How Might Climate Change Have Affected Erosion Rates?

    NASA Astrophysics Data System (ADS)

    Molnar, Peter

    2004-05-01

    Accumulation rates of terrestrial sediment have increased in the past few million years both on and adjacent to continents, although not everywhere. Apparently, erosion has increased in elevated terrain regardless of when last tectonically active or what the present-day climate. In many regions, sediment coarsened abruptly in late Pliocene time. Sparser data suggest increased sedimentation rates at 15 Ma, approximately when oxygen isotopes in benthic foraminifera imply high-latitude cooling. If climate change effected accelerated erosion, understanding how it did so remains the challenge. Some obvious candidates, such as lowered sea level leading to erosion of continental shelves or increased glaciation, account for increased sedimentation in some, but not all, areas. Perhaps stable climates that varied slowly allowed geomorphic processes to maintain a state of equilibrium with little erosion until 34 Ma, when large oscillations in climate with periods of 20,00040,000 years developed and denied the landscape the chance to reach equilibrium.

  11. [Dynamics simulation on plant growth, N accumulation and utilization of processing tomato at different N fertilization rates].

    PubMed

    Wang, Xin; Ma, Fu-Yu; Diao, Ming; Fan, Huam; Cui, Jing; Jia, Biao; He, Hai-Bing; Liu, Qi

    2014-04-01

    Three field experiments were conducted to simulate the dynamics of aboveground biomass, N accumulation and utilization of drip-irrigated processing tomatoes at different N fertilization rates (0, 75, 150, 300, 450, or 600 kg x hm(-2)). The results showed that Logistic models best described the changes in aboveground biomass, N accumulation, and utilization of accumulated N efficiency with the physiological development time (PDT). Rapid accumulation of N began about 4-6 d (PDT) earlier than the rapid accumulation of aboveground biomass. The momentary utilization rate of N (NMUR) increased after emergence, reached a single peak, and then decreased. The N accumulation, aboveground biomass and yield were highest in the 300 kg x hm(-2) treatment. The quadratic model indicated that application rate of 349 to 382 kg N x hm(-2) was optimum for drip-irrigated processing tomatoes in northern Xinjiang. PMID:25011297

  12. Constraining mass accumulation rates across the Cretaceous-Paleogene boundary clay layer using extraterrestrial helium-3

    NASA Astrophysics Data System (ADS)

    Giron, M.; Sepulveda, J.; Mukhopadhyay, S.; Alegret, L.; Summons, R. E.

    2012-12-01

    The extended duration of the negative δ13C excursion observed in marine carbonates spanning the Cretaceous-Paleogene (K-Pg) mass extinction event has lead to two main hypothesized post-extinction models ("Strangelove" and "Living Ocean";[1, 2]) for the status of marine primary productivity and the global carbon cycle. However, these models are largely inconsistent with recent paleontological and geochemical evidence suggesting heterogeneous changes in marine productivity and carbon export [3, 4]. While the analysis of lipid biomarkers in the cosmopolitan boundary clay layer allows us to assess changes in primary production by non-calcifying organisms in the immediate aftermath of the mass extinction [4], our poor understanding of the deposition of the clay layer precludes a more detailed reconstruction of short-term variations in marine ecosystem resilience. Here, we present data on extraterrestrial 3He derived from interplanetary dust particles used as a constant flux proxy to constrain fluctuations in mass accumulation rates (MARs) [5] and the duration of the boundary clay deposition in three classic and expanded K-Pg boundary sections: El Kef (Tunisia), Caravaca (Spain), and Kulstirenden (Denmark). Our results from different depositional environments indicate average durations for the sedimentation of the clay layer that are comparable (~10 kyr) to other localities [5], thus confirming its globally brief deposition. Early Paleogene MARs vary among locations when compared to background Late Cretaceous values and do not strictly follow carbonate content as traditionally assumed, thus suggesting variable depositional conditions at different locations. Changes in sediment MARs across the K-Pg will be used to calculate MARs of algal- and bacterial-derived biomarkers, as well as benthic foraminifera, in order to assess the timing and global nature of the recovery of marine primary production and carbon export. 1. Hsu, K.J., He, Q., Mckenzie, J.A., Weissert, H

  13. Unprecedented last-glacial mass accumulation rates determined by luminescence dating of loess from western Nebraska

    USGS Publications Warehouse

    Roberts, H.M.; Muhs, D.R.; Wintle, A.G.; Duller, G.A.T.; Bettis, E. Arthur

    2003-01-01

    A high-resolution chronology for Peoria (last glacial period) Loess from three sites in Nebraska, midcontinental North America, is determined by applying optically stimulated luminescence (OSL) dating to 35-50 ??m quartz. At Bignell Hill, Nebraska, an OSL age of 25,000 yr near the contact of Peoria Loess with the underlying Gilman Canyon Formation shows that dust accumulation occurred early during the last glacial maximum (LGM), whereas at Devil's Den and Eustis, Nebraska, basal OSL ages are significantly younger (18,000 and 21,000 yr, respectively). At all three localities, dust accumulation ended at some time after 14,000 yr ago. Mass accumulation rates (MARs) for western Nebraska, calculated using the OSL ages, are extremely high from 18,000 to 14,000 yr-much higher than those calculated for any other pre-Holocene location worldwide. These unprecedented MARs coincide with the timing of a mismatch between paleoenvironmental evidence from central North America, and the paleoclimate simulations from atmospheric global circulation models (AGCMs). We infer that the high atmospheric dust loading implied by these MARs may have played an important role, through radiative forcing, in maintaining a colder-than-present climate over central North America for several thousand years after summer insolation exceeded present-day values. ?? 2003 Elsevier Science (USA). All rights reserved.

  14. Proportional accumulation of yolk proteins derived from multiple vitellogenins is precisely regulated during vitellogenesis in striped bass (Morone saxatilis).

    PubMed

    Williams, Valerie N; Reading, Benjamin J; Amano, Haruna; Hiramatsu, Naoshi; Schilling, Justin; Salger, Scott A; Islam Williams, Taufika; Gross, Kevin; Sullivan, Craig V

    2014-07-01

    We quantified three vitellogenins (VtgAa, VtgAb, VtgC) or their derived yolk proteins (YPs) in the liver, plasma, and ovary during pre-vitellogenic (PreVG), mid-vitellogenic (MVG), and late-vitellogenic (LVG) oocyte growth and during post-vitellogenesis (PostVG) in the striped bass (Morone saxatilis) using label-free quantitative mass spectrometry (MS). Western blotting of the samples using antisera raised against gray mullet (Mugil cephalus) lipovitellins derived from VtgAa, VtgAb, and VtgC confirmed the MS results. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed liver as the primary site of expression for all three Vtgs, with extra-hepatic transcription weakly detected in ovary, foregut, adipose tissue, and brain. Quantitative real-time RT-PCR confirmed vtgAb to be primarily expressed in liver and VtgAb proteins were predominant in liver and plasma from MVG to PostVG. However, the primary period of deposition into oocytes of VtgAb occurred up until MVG, whereas VtgAa was primarily deposited from MVG to LVG. The VtgC was gradually taken up by oocytes throughout vitellogenesis and was detected at trace levels in plasma. The ratio of yolk proteins derived from VtgAa, VtgAb, VtgC (YPAa/YPAb/YPC) in PostVG ovary is 1.4:1.4:1, which differs from ratios previously reported for other fish species in that YPC comprises a greater proportion of the egg yolk. Our results indicate that proportional accumulation of multiple Vtgs in the yolk may depend both on the precise rates of their hepatic secretion and specific uptake by oocytes. Furthermore, composition of the Vtg-derived yolk may vary among Acanthomorph fishes, perhaps reflecting their different early life histories and reproductive strategies. PMID:24648375

  15. Proportional accumulation of yolk proteins derived from multiple vitellogenins is precisely regulated during vitellogenesis in striped bass (Morone saxatilis).

    PubMed

    Williams, Valerie N; Reading, Benjamin J; Amano, Haruna; Hiramatsu, Naoshi; Schilling, Justin; Salger, Scott A; Islam Williams, Taufika; Gross, Kevin; Sullivan, Craig V

    2014-07-01

    We quantified three vitellogenins (VtgAa, VtgAb, VtgC) or their derived yolk proteins (YPs) in the liver, plasma, and ovary during pre-vitellogenic (PreVG), mid-vitellogenic (MVG), and late-vitellogenic (LVG) oocyte growth and during post-vitellogenesis (PostVG) in the striped bass (Morone saxatilis) using label-free quantitative mass spectrometry (MS). Western blotting of the samples using antisera raised against gray mullet (Mugil cephalus) lipovitellins derived from VtgAa, VtgAb, and VtgC confirmed the MS results. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) revealed liver as the primary site of expression for all three Vtgs, with extra-hepatic transcription weakly detected in ovary, foregut, adipose tissue, and brain. Quantitative real-time RT-PCR confirmed vtgAb to be primarily expressed in liver and VtgAb proteins were predominant in liver and plasma from MVG to PostVG. However, the primary period of deposition into oocytes of VtgAb occurred up until MVG, whereas VtgAa was primarily deposited from MVG to LVG. The VtgC was gradually taken up by oocytes throughout vitellogenesis and was detected at trace levels in plasma. The ratio of yolk proteins derived from VtgAa, VtgAb, VtgC (YPAa/YPAb/YPC) in PostVG ovary is 1.4:1.4:1, which differs from ratios previously reported for other fish species in that YPC comprises a greater proportion of the egg yolk. Our results indicate that proportional accumulation of multiple Vtgs in the yolk may depend both on the precise rates of their hepatic secretion and specific uptake by oocytes. Furthermore, composition of the Vtg-derived yolk may vary among Acanthomorph fishes, perhaps reflecting their different early life histories and reproductive strategies.

  16. Modified lipoprotein-derived lipid particles accumulate in human stenotic aortic valves.

    PubMed

    Lehti, Satu; Käkelä, Reijo; Hörkkö, Sohvi; Kummu, Outi; Helske-Suihko, Satu; Kupari, Markku; Werkkala, Kalervo; Kovanen, Petri T; Oörni, Katariina

    2013-01-01

    In aortic stenosis plasma lipoprotein-derived lipids accumulate in aortic valves. Here, we first compared the lipid compositions of stenotic aortic valves and atherosclerotic plaque cores. Both pathological tissues were found to be enriched in cholesteryl linoleate, a marker of extracellularly accumulated lipoproteins. In addition, a large proportion of the phospholipids were found to contain arachidonic acid, the common precursor of a number of proinflammatory lipid mediators. Next, we isolated and characterized extracellular lipid particles from human stenotic and non-stenotic control valves, and compared them to plasma lipoproteins from the same subjects. The extracellular valvular lipid particles were isolated from 15 stenotic and 14 non-stenotic aortic valves. Significantly more apoB-100-containing lipid particles were found in the stenotic than in the non-stenotic valves. The majority of the lipid particles isolated from the non-stenotic valves had sizes (23±6.2 nm in diameter) similar to those of plasma low density lipoprotein (LDL) (22±1.5 nm), while the lipid particles from stenotic valves were not of uniform size, their sizes ranging from 18 to more than 500 nm. The lipid particles showed signs of oxidative modifications, and when compared to isolated plasma LDL particles, the lipid particles isolated from the stenotic valves had a higher sphingomyelin/phosphatidylcholine -ratio, and also higher contents of lysophosphatidylcholine and unesterified cholesterol. The findings of the present study reveal, for the first time, that in stenotic human aortic valves, infiltrated plasma lipoproteins have undergone oxidative and lipolytic modifications, and become fused and aggregated. The generated large lipid particles may contribute to the pathogenesis of human aortic stenosis.

  17. Relative cooling rates derived from basalt column geometries

    NASA Astrophysics Data System (ADS)

    Woodell, Daniel; Porritt, Lucy; Russell, Kelly

    2015-04-01

    Columnar joints form as a brittle relaxation response to tensile stresses that accumulate during cooling of lava flows, pyroclastic deposits, and intrusive magma bodies. Columnar jointing forms in different deposit types, in deposits of different compositions, and different outcrop geometries. Despite this diversity, columns follow a few "rules": column diameter is inversely proportional to cooling rate (small/quick cooling times, small diameter columns), columns only ever coalesce (never bifurcate), and columnar joints always propagate parallel to but in the opposite direction of heat flow (towards the hottest part of the flow). Using these "rules," cooling histories and emplacement environments can be reconstructed. While column geometries and definitions of various columnar structures vary between authors (upper and lower colonnade and entablature vs. master cracks and pseudopillows), this study focuses on relatively simple outcrops of basalt lava within the Cheakamus River valley near Whistler, BC, Canada. The basalt lavas described here, thought to have erupted subglacially, contain columns comprising only well-defined upper and lower colonnades (i.e., no entablature). Comparing the relative thicknesses of upper and lower colonnades reveals the cooling history, relative cooling rates, and amounts of heat transferred from the upper and lower flow boundaries. Forward numerical models using the finite element method are created with Matlab using the Partial Differential Equation Toolbox to model the outcrops in the Whistler field area, and determine the cooling rates and thermal gradients experienced by the lava flows during their formation. This study finds that noticeable differences in the thickness of upper and lower colonnades within an outcrop occur only when there are large differences in cooling rates between the upper and lower flow surfaces. Modeling shows that the cooling rates must differ by approximately an order of magnitude to produce the observed

  18. A PDE Pricing Framework for Cross-Currency Interest Rate Derivatives with Target Redemption Features

    NASA Astrophysics Data System (ADS)

    Christara, Christina C.; Minh Dang, Duy; Jackson, Kenneth R.; Lakhany, Asif

    2010-09-01

    We propose a general framework for efficient pricing via a partial differential equation (PDE) approach for exotic cross-currency interest rate (IR) derivatives, with strong emphasis on long-dated foreign exchange (FX) IR hybrids, namely Power Reverse Dual Currency (PRDC) swaps with a FX Target Redemption (FX-TARN) provision. The FX-TARN provision provides a cap on the FX-linked PRDC coupon amounts, and once the accumulated coupon amount reaches this cap, the underlying PRDC swap terminates. Our PDE pricing framework is based on an auxiliary state variable to keep track of the total accumulated PRDC coupon amount. Finite differences on uniform grids and the Alternating Direction Implicit (ADI) method are used for the spatial and time discretizations, respectively, of the model-dependent PDE corresponding to each discretized value of the auxiliary variable. Numerical examples illustrating the convergence properties of the numerical methods are provided.

  19. Distributions and accumulation rates of polycyclic aromatic hydrocarbons in the northern Gulf of Mexico sediments.

    PubMed

    Adhikari, Puspa L; Maiti, Kanchan; Overton, Edward B; Rosenheim, Brad E; Marx, Brian D

    2016-05-01

    Sediment samples collected from shelf, slope and interior basin of the northern Gulf of Mexico during 2011-2013, 1-3 years after the Deepwater Horizon (DWH) oil spill, were utilized to characterize PAH pollution history, in this region. Results indicate that the concentrations of surface ΣPAH43 and their accumulation rates vary between 44 and 160 ng g(-1) and 6-55 ng cm(-2) y(-1), respectively. ΣPAH43 concentration profiles, accumulation rates and Δ(14)C values are significantly altered only for the sediments in the immediate vicinity of the DWH wellhead. This shows that the impact of DWH oil input on deep-sea sediments was generally limited to the area close to the spill site. Further, the PAHs source diagnostic analyses suggest a noticeable change in PAHs composition from higher to lower molecular weight dominance which reflects a change in source of PAHs in the past three years, back to the background composition. Results indicate low to moderate levels of PAH pollution in this region at present, which are unlikely to cause adverse effects on benthic communities. PMID:26895564

  20. Field studies using the oyster Crassostrea virginica to determine mercury accumulation and depuration rates

    SciTech Connect

    Palmer, S.J.; Presley, B.J.; Powell, E.N. ); Taylor, R.J. )

    1993-09-01

    Mercury as an environmental hazard, especially with regard to human health, has been of concern since the Minamata disaster. From 1966 to 1970 a chlor-alkali plant in Point Comfort, Texas released mercury-enriched wastewater (up to 29.9 kgHg/day) into Lavaca Bay (TWQB 1977). Since 1970 the Texas Department of Health (TDH) has periodically closed and then re-opened portions of Lavaca Bay to the harvesting of crabs and finfish based on their levels (<>0.5 ppm Hg wet weight) of mercury. A 1988 closure remains in effect as of this writing. Mercury contamination in Lavaca Bay organisms thus continues to be a problem 22 years after the chlor-alkali plant ceased releasing mercury into the bay. The goal of the following research was to better understand the behavior of mercury in Lavaca Bay. Oysters have been widely used as indicator species in metal pollution studies. Most such programs have focused on the concentrations of metals in oysters from different geographic areas. This study, however, investigated the rate and amount of mercury a [open quotes]clean[close quotes] oyster would accumulate when transplanted to a contaminated estuary and the rate of mercury depuration by contaminated oysters placed in a clean environment. The oysters were additionally analyzed for Ba, Cu, Fe, P, and Zn to test for the possible involvement of these metals in mercury accumulation and depuration. 17 refs., 3 figs., 2 tabs.

  1. An automatic method for deriving steady-state rate equations.

    PubMed Central

    Cornish-Bowden, A

    1977-01-01

    A method is described for systematically deriving steady-state rate equations. It is based on the schematic method of King & Altman [J. Phys. Chem. (1956) 60, 1375-1378], but is expressed in purely algebraic terms. It is suitable for implementation as a computer program, and a program has been written in FORTRAN IV and deposited as Supplementary Publication SUP 50078 (12 pages) at the British Library (Lending Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1977) 161, 1-2. PMID:889575

  2. Complex Wind-Induced Variations of Surface Snow Accumulation Rates over East Antarctica

    NASA Astrophysics Data System (ADS)

    Das, I.; Scambos, T. A.; Koenig, L.; van den Broeke, M.; Lenaerts, J.

    2015-12-01

    Accurate quantification of surface snow-accumulation over Antarctica is important for mass balance estimates and climate studies based on ice core records. Using airborne radar, lidar and thresholds of surface slope, modeled surface mass balance (SMB) and wind fields, we have predicted continent-wide distribution of wind-scour zones over Antarctica. These zones are located over relatively steep ice surfaces formed by ice flow over bedrock topography. Near-surface winds accelerate over these steeper slopes and erode and sublimate the snow. This results in numerous localized regions (typically ≤ 200 km2) with reduced or negative surface accumulation. Although small zones of re-deposition occur at the base of the steeper slope areas, the redeposited mass is small relative to the ablation loss. Total losses from wind-scour and wind-glaze areas amounts to tens of gigatons annually. Near the coast, winds often blow significant amounts of surface snow from these zones into the ocean. Large uncertainties remain in SMB estimates over East Antarctica as climate models do not adequately represent the small-scale physical processes that lead to mass loss or redistribution over the wind-scour zones. In this study, we also use Operation IceBridge's snow radar data to provide evidence for a gradual ablation of ~16-18 m of firn (~200 years of accumulation) from wind-scour zones over the upper Recovery Ice Stream catchment. The maximum ablation rates observed in this region are ~ -54 kg m-2 a-1 (-54 mm water equivalent a-1). Our airborne radio echo-sounding analysis show snow redeposition downslope of the wind-scour zones is <10% of the cumulative mass loss. Our study shows that the local mass loss is dominated by sublimation to water vapor rather than wind-transport of snow.

  3. A comparison of recent, short-, and long-term carbon accumulation rates for a vegetation gradient in central Alaska

    NASA Astrophysics Data System (ADS)

    Manies, K.; Harden, J. W.; Turetsky, M.; Fuller, C.

    2013-12-01

    Information regarding historical rates of carbon (C) accumulation will aid scientists in understanding how climate change may affect biogeochemical cycles in the future. We examined rates of C accumulation for the following three time periods: the last two thousand years (long-term rates), the last 30 years (short-term rates), and the last several years (recent rates). We compared C accumulation rates among these time periods for five different ecosystems found along a ~300-m transect within the Bonanza Creek Long-term Ecological Research (LTER) site. These sites were dominated by black spruce, low shrubs, tussock grass, Carex sp., or brown moss. The black spruce and shrub site are the only ecosystems currently underlain by permafrost. Three soil cores were taken at each site and analyzed for C content. In order to gain a robust understanding of C accumulation rates at each site, 14C measurements and 210Pb chronologies were also obtained, and flux measurements were taken at each site. 14C dates were acquired for the basal horizon of one profile for each ecosystem type, providing estimations of C accumulation rates since organic matter began to form. 210Pb chronologies for each soil profile allowed us to estimate C accumulation rates for the last several decades. Finally, CO2 flux measurements were taken at each site from May - September for five years (2007 - 2011), capturing recent C losses and gains. Although short-term C accumulation rates were lowest in the black spruce ecosystem, rates among ecosystems were not significantly different, due to large variability among soil profiles within each site (coefficient of variations of up to 50%). The long-term C accumulation rate at the black spruce site corresponds well to values measured in an adjacent black spruce forest using eddy covariance. The brown moss site had the highest long-term rates of C accumulation among the five ecosystems. Short-term C accumulation rates were always higher than long-term rates (40

  4. Engineering alfalfa to accumulate useful caffeic acid derivatives and characterization of hydroxycinnamoyl-CoA transferases from legumes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Some forages crops, such as red clover, accumulate high levels of caffeic acid derivatives. Oxidation of these o-diphenols to quinones by endogenous polyphenol oxidases (PPOs) and the subsequent reactions of these quinones (probably with endogenous plant proteases) result in a significant reduction ...

  5. Plant regeneration and biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in Hypoxis hemerocallidea organ and callus cultures.

    PubMed

    Moyo, Mack; Amoo, Stephen O; Aremu, Adeyemi O; Gruz, Jiří; Subrtová, Michaela; Doležal, Karel; Van Staden, Johannes

    2014-10-01

    Micropropagation of Hypoxis hemerocallidea Fisch. and C.A. Mey was used as a model system to study the influence of cytokinins (CKs) on plant regeneration and biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in organ and callus cultures and their antioxidant activity. Fourteen free phenolic acids were detected using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) while antioxidant activity was evaluated using oxygen radical absorbance capacity (ORAC) and 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity. Cytokinins had a significant effect on the biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in H. hemerocallidea organ cultures. In particular, meta-topolin-treated organ cultures produced high concentrations of gallic, protocatechuic, gentisic, p-hydroxybenzoic, m-hydroxybenzoic, salicylic, chlorogenic and trans-cinnamic acids. The isoprenoid CK, N(6)-(2-isopentenyl)-adenine significantly increased the accumulation of hydroxycinnamic acid derivatives, namely, caffeic, p-coumaric, sinapic and ferulic acids. Cytokinin-treated organ cultures exhibited a significant increase in antioxidant activity, particularly in the ORAC model. In callus cultures, CKs decreased the concentrations of hydroxycinnamic acid derivatives and antioxidant activity when compared to the control. Overall, both CK type and concentration had a significant effect on plant regeneration, callus proliferation, biochemical accumulation of free phenolic acids and antioxidant activity of the resultant extracts.

  6. A closer look at the Neogene erosion and accumulation rate increase

    NASA Astrophysics Data System (ADS)

    Willenbring, J.; von Blanckenburg, F.

    2008-12-01

    Glacial erosion and Quaternary cold-stage warm-stage climate cycling have been cited as mechanisms to explain observations of increased Neogene marine sedimentation rates. Quantification of long-term glacial erosion rates from cosmogenic radionuclides from large areas mostly covered by cold-based ice during the Quaternary show very low erosion rates over several glacial cycles. In addition, isotope ratio proxies of dissolved metals in seawater, measured in chemical ocean sediments, lack clear evidence for an increase in terrigenous denudation. In particular, the stable isotope 9Be, derived from continental erosion, shows no change in its ratio to meteoric cosmogenic nuclide 10Be, derived from rain over the past 10 My. Radiogenic Pb and Nd isotopes, mainly show a change in the style of denudation from more chemical to more physical processes in the Quaternary. These data are at odds with a suggested increase in marine sedimentation rates during the late Cenozoic. In order to resolve this contradiction we have scrutinized these sedimentation rate calculations from ocean cores to identify whether they might show only apparent increases in the Neogene sections. Potential explanations are that in some cases, measured sediment thicknesses for different time intervals lack corrections for sediment compaction. Compaction of the lower portions of the cores drastically increases the apparent thickness of the more recent (Quaternary) sediment. In addition, sedimentation rates often only appear higher for recent sections in cores due to an artifact of an averaging timescale that decreases up-core. Such an averaging time scale decrease arises from better chronological resolution in recent times (Sadler et al., 1999). Cannibalization of older sediment might add to this effect. Together, these data question a clear, global-scale Quaternary climate-erosion connection that would be unique in Earth's history.

  7. A molecular assessment of phylogenetic relationships and lineage accumulation rates within the family Salamandridae (Amphibia, Caudata).

    PubMed

    Weisrock, David W; Papenfuss, Theodore J; Macey, J Robert; Litvinchuk, Spartak N; Polymeni, Rosa; Ugurtas, Ismail H; Zhao, Ermi; Jowkar, Houman; Larson, Allan

    2006-11-01

    We examine phylogenetic relationships among salamanders of the family Salamandridae using approximately 2700 bases of new mtDNA sequence data (the tRNALeu, ND1, tRNAIle, tRNAGln, tRNAMet, ND2, tRNATrp, tRNAAla, tRNAAsn, tRNACys, tRNATyr, and COI genes and the origin for light-strand replication) collected from 96 individuals representing 61 of the 66 recognized salamandrid species and outgroups. Phylogenetic analyses using maximum parsimony and Bayesian analysis are performed on the new data alone and combined with previously reported sequences from other parts of the mitochondrial genome. The basal phylogenetic split is a polytomy of lineages ancestral to (1) the Italian newt Salamandrina terdigitata, (2) a strongly supported clade comprising the "true" salamanders (genera Chioglossa, Mertensiella, Lyciasalamandra, and Salamandra), and (3) a strongly supported clade comprising all newts except S. terdigitata. Strongly supported clades within the true salamanders include monophyly of each genus and grouping Chioglossa and Mertensiella as the sister taxon to a clade comprising Lyciasalamandra and Salamandra. Among newts, genera Echinotriton, Pleurodeles, and Tylototriton form a strongly supported clade whose sister taxon comprises the genera Calotriton, Cynops, Euproctus, Neurergus, Notophthalmus, Pachytriton, Paramesotriton, Taricha, and Triturus. Our results strongly support monophyly of all polytypic newt genera except Paramesotriton and Triturus, which appear paraphyletic, and Calotriton, for which only one of the two species is sampled. Other well-supported clades within newts include (1) Asian genera Cynops, Pachytriton, and Paramesotriton, (2) North American genera Notophthalmus and Taricha, (3) the Triturus vulgaris species group, and (4) the Triturus cristatus species group; some additional groupings appear strong in Bayesian but not parsimony analyses. Rates of lineage accumulation through time are evaluated using this nearly comprehensive sampling of

  8. Verification of International Space Station Component Leak Rates by Helium Accumulation Method

    NASA Technical Reports Server (NTRS)

    Underwood, Steve D.; Smith, Sherry L.

    2003-01-01

    Discovery of leakage on several International Space Station U.S. Laboratory Module ammonia system quick disconnects (QDs) led to the need for a process to quantify total leakage without removing the QDs from the system. An innovative solution was proposed allowing quantitative leak rate measurement at ambient external pressure without QD removal. The method utilizes a helium mass spectrometer configured in the detector probe mode to determine helium leak rates inside a containment hood installed on the test component. The method was validated through extensive developmental testing. Test results showed the method was viable, accurate and repeatable for a wide range of leak rates. The accumulation method has been accepted by NASA and is currently being used by Boeing Huntsville, Boeing Kennedy Space Center and Boeing Johnson Space Center to test welds and valves and will be used by Alenia to test the Cupola. The method has been used in place of more expensive vacuum chamber testing which requires removing the test component from the system.

  9. Comparisons of 210Pb and pollen methods for determining rates of estuarine sediment accumulation

    USGS Publications Warehouse

    Brush, G.S.; Martin, E.A.; DeFries, R.S.; Rice, C.A.

    1982-01-01

    Comparisons of sedimentation rates obtained by 210Pb and pollen analyses of 1-m cores collected throughout the Potomac Estuary show good agreement in the majority of cores that can be analyzed by both methods. Most of the discrepancy between the methods can be explained by the analytical precision of the 210Pb method and by the exactness with which time horizons can be identified and dated for the pollen method. X-radiographs of the cores and the distinctness of the pollen horizons preclude significant displacement by reworking and/or mixing of sediments. Differences between the methods are greatest where uncertainties exist in assigning a rate by one or both methods (i.e., 210Pb trends and/or "possible" horizon assignments). Both methods show the same relative rates, with greater sediment accumulation more common in the upper and middle estuary and less toward the mouth. The results indicate that geochronologic studies of estuarine sediments should be preceded by careful observation of sedimentary structures, preferably by X-radiography, to evaluate the extent of mixing of the sediments. Time horizons, whether paleontologic or isotopic, are generally blurred where mixing has occurred, precluding precise identification. Whenever possible, two methods should be used for dating sediments because a rate, albeit erroneous, can be obtained isotopically in sediments that are mixed; accurate sedimentation rates are also difficult to determine where the time boundary is a zone rather than a horizon, where the historical record does not provide a precise date for the pollen horizon, or where scouring has removed some of the sediment above a dated pollen horizon. ?? 1982.

  10. Deriving Time Series of Ice-Sheet Accumulation Variations from Altimeter Measurements of Surface-Elevation Changes

    NASA Astrophysics Data System (ADS)

    Zwally, H. J.; Li, J.

    2014-12-01

    Information on ice-sheet surface accumulation, A(t), is needed for studies of mass balance, polar climate variability, ice-sheet evolution, and dynamics. This paper describes a new method for deriving time series of A(t) variations, δA(t), on a monthly basis from continuous altimeter measurements. Changes in ice-sheet thickness and surface elevation are driven by a combination of δA(t), changes in the rates of firn compaction, and the rates of dynamic ice thickening or thinning (dHd/dt). Relevant time scales range from monthly fluctuations in δA(t) and surface temperature, Ts(t), to decadal to millennial scales of dynamic changes in ice flow. Our concept is based on the widely differing time-scales of principal processes, e.g. T> 5 years for dynamic changes and t = year/12 for δA(t) so that T >> t. An important aspect is our use of a baseline of ICESat-1 and ERS-1/ERS-2 altimeter measurements to obtain the multi-year (e.g. 5 to 17 years) dHd/dt of the Antarctic and Greenland ice sheets. We calculate changes in surface elevations caused by variations in the rate of firn compaction driven by both δA(t) and Ts(t) to adjust the altimeter-observed height changes, dH/dt, in order to derive dHd/dt over time periods of ≥ 5 years. Although monthly values of both δA(t) and Ts(t) are used, the derived dHd/dt is dependent only on the average change in δA(t) over the period and not on the higher-frequency monthly δA(t). On a monthly basis, the total accumulation-driven height change, dHaCA/dt, is a sum of the direct-height change, dHa/dt, and the change in firn compaction, dCA/dt, both caused by δA(t). dHa/dt is equal to δA(t)/ρs, where ρs is relative density of surface snow (typically 0.3) and δA(t) is w.e. Model simulations of dHaCA/dt driven by monthly δA(t) from ERA-interim re-analyses show that altimeter-observable dHaCA/dt and the desired dHa/dt = δA(t)/ρs are closely related. The relationship is better at colder locations such as the South Pole (Tm

  11. Host-Derived Smooth Muscle Cells Accumulate in Cardiac Allografts: Role of Inflammation and Monocyte Chemoattractant Protein 1

    PubMed Central

    Bojakowski, Krzysztof; Soin, Joanna; Nozynski, Jerzy; Zakliczynski, Michal; Gaciong, Zbigniew; Zembala, Marian; Söderberg-Nauclér, Cecilia

    2009-01-01

    Transplant arteriosclerosis is characterized by inflammation and intimal thickening caused by accumulation of smooth muscle cells (SMCs) both from donor and recipient. We assessed the relationship between clinical factors and the presence of host-derived SMCs in 124 myocardial biopsies from 26 consecutive patients who received hearts from opposite-sex donors. Clinical and demographic information was obtained from the patients' medical records. Host-derived SMCs accounted for 3.35±2.3% of cells in arterioles (range, 0.08–12.51%). As shown by linear regression analysis, an increased number of SMCs was associated with rejection grade (mean, 1.41±1.03, p = 0.034) and the number of leukocytes (19.1±12.7 per 20 high-power fields, p = 0.01). The accumulation of host-derived SMCs was associated with an increased number of leukocytes in the allografts. In vitro, monocyte chemoattractant protein 1 (MCP-1) released from leukocytes was crucial for SMC migration. After heart allotransplantion, mice treated with MCP-1-specific antibodies had significantly fewer host-derived SMCs in the grafts than mice treated with isotypic antibody controls. We conclude that the number of host-derived SMCs in human cardiac allografts is associated with the rejection grade and that MCP-1 may play pivotal role in recruiting host-derived SMCs into cardiac allografts. PMID:19142231

  12. Millennial Rates of Sea Cliff Retreat Derived From Cosmogenic 10Be and Coastal Platform Morphology

    NASA Astrophysics Data System (ADS)

    Hurst, M. D.; Ellis, M. A.; Rood, D. H.

    2014-12-01

    Observation of cliff erosion are often limited to relatively short timescales (a few decades), which are within the timeframe of anthropogenic modification of the coast and may be shorter than the recurrence interval for erosion events. Here we present long-term (centennial-millennial) averaged rates of sea cliff retreat for chalk cliffs in SE England derived from cosmogenic isotopes and coastal morphology. We determine long-term rates of sea cliff erosion from 10Be measured from in situ flint samples collected from three transects across the coastal platform in East Sussex. A numerical model of 10Be accumulation on an evolving coastal profile allows estimation of cliff retreat rate averaged over several hundred years. The model accounts for variation in 10Be accumulation with tides and sea-level rise, and takes into account platform downwear and topographic shielding by adjacent cliffs. Additionally, we use high-resolution (1m) multibeam bathymetry to map the extent of the coastal platform based on the surface texture in order to infer the position of the coast at ~8 ka. The difference in position to the current coastline provides estimates of Holocene-averaged rates of cliff erosion for all chalk cliffed coastline in the region. Comparison to historic records of cliff retreat reveals key similarities and differences between long and short-term signals. In certain locations, there are significant discrepancies (either faster or slower) between historic records and long-term rates of retreat. Each type of discrepancy may be the result of human interaction with the coastal environment, whether that interaction is local or non-local, and it is worthwhile noting that sites of relatively low historic rates of erosion are likely subject to high-magnitude, low-frequency failure events that could have devastating effects on human lives and infrastructure in areas that are considered to be low risk.

  13. Tracing nitrogen accumulation in decaying wood and examining its impact on wood decomposition rate

    NASA Astrophysics Data System (ADS)

    Rinne, Katja T.; Rajala, Tiina; Peltoniemi, Krista; Chen, Janet; Smolander, Aino; Mäkipää, Raisa

    2016-04-01

    Decomposition of dead wood, which is controlled primarily by fungi is important for ecosystem carbon cycle and has potentially a significant role in nitrogen fixation via diazotrophs. Nitrogen content has been found to increase with advancing wood decay in several studies; however, the importance of this increase to decay rate and the sources of external nitrogen remain unclear. Improved knowledge of the temporal dynamics of wood decomposition rate and nitrogen accumulation in wood as well as the drivers of the two processes would be important for carbon and nitrogen models dealing with ecosystem responses to climate change. To tackle these questions we applied several analytical methods on Norway spruce logs from Lapinjärvi, Finland. We incubated wood samples (density classes from I to V, n=49) in different temperatures (from 8.5oC to 41oC, n=7). After a common seven day pre-incubation period at 14.5oC, the bottles were incubated six days in their designated temperature prior to CO2 flux measurements with GC to determine the decomposition rate. N2 fixation was measured with acetylene reduction assay after further 48 hour incubation. In addition, fungal DNA, (MiSeq Illumina) δ15N and N% composition of wood for samples incubated at 14.5oC were determined. Radiocarbon method was applied to obtain age distribution for the density classes. The asymbiotic N2 fixation rate was clearly dependent on the stage of wood decay and increased from stage I to stage IV but was substantially reduced in stage V. CO2 production was highest in the intermediate decay stage (classes II-IV). Both N2 fixation and CO2 production were highly temperature sensitive having optima in temperature 25oC and 31oC, respectively. We calculated the variation of annual levels of respiration and N2 fixation per hectare for the study site, and used the latter data together with the 14C results to determine the amount of N2 accumulated in wood in time. The proportion of total nitrogen in wood

  14. Recent Carbon Accumulation Rates in Ombrotrophic Peatlands on the North Shore of the Gulf of the St. Lawrence, Quebec, Canada

    NASA Astrophysics Data System (ADS)

    Sanderson, N. K.; Charman, D.; Garneau, M.; Hartley, I. P.

    2013-12-01

    In Eastern Canada, permafrost reached its southernmost extent for the Holocene during the Little Ice Age. Recent warming and degradation along the southern limit of the discontinuous permafrost zone is altering the microtopography, hydrology and carbon cycling in ombrotrophic peatlands; this trend is projected to continue. However, the understanding of multi-decadal and centennial scale change in peatlands is limited. This study aims to quantify changes in carbon accumulation rates for the last millennium in ombrotrophic peatlands from three regions along the North Shore of the Gulf of the St Lawrence, Canada. In each region, three peatlands were cored and 3-4 microforms were sampled per peatland. This replication allows changes in accumulation rates to be examined on two scales: 1) between regions along a N-S climatic gradient, and 2) within peatlands in the same region along a microtopography gradient. Recent carbon accumulation rates for last 150-200 years were calculated with lead-210 dates. Some initial estimates are also available for the last millennium using radiocarbon dates. Carbon accumulation rates were higher for all sites in the southernmost region, with sphagnum hummocks having the recent highest accumulation rates overall. In more northern peatlands, rates were found to differ between microforms, and between sites. This variability may be due to local differences in wind exposure and winter snow cover between sites. A high-resolution (0.5 cm) multi-proxy analysis using testate amoebae and plant macrofossils will be performed around key periods of accumulation change to evaluate peatland sensitivity and carbon accumulation to hydrological change.

  15. Sediment accumulation rates in Conowingo Reservoir as determined by man-made and natural radionuclides

    SciTech Connect

    McLean, R.I.; Domotor, S.L. ); Summers, J.K.; Wilson, H. ); Olsen, C.R.; Larsen, I.L. )

    1991-05-01

    The Susquehanna River is the major contributor to sediment loadings in the Chesapeake Bay. Because many environmental contaminants are associated with suspended particulates, the degree of particle retention within the reservoirs of the lower Susquehanna River is an important consideration in evaluating contaminant loadings to the Chesapeake Bay. Profiles of weapons-test Cs-137, nuclear power plant-related Cs-134 and Cs-137, and naturally-derived Pb-210 were used to estimate rates of sediment accretion in the Conowingo Reservoir,an impoundmment of the Susquehanna River along the Maryland-Pennsylvania border. Net accretion rates ranged from about 2 cm yr{sup {minus}1} downstream of a nuclear power plant cooling discharge to a high of about 7 cm yr{sup {minus}1} at the mount of an incoming creek. Slight, but consistent, increases in the annual rate of accretion since the creation of the reservoir in 1928 are apparent. The current net average annual sediment load retained by the reservoir is estimated to be 0.4 {times} 10{sup 6} to 1.5 {times} 10{sup 6} metric tons yr{sup {minus}1}. The retained sediment load represents about 8-23% of the long-time average sediment input to the reservoir.

  16. Characteristics of the accumulation of methotrexate polyglutamate derivatives in Ehrlich ascites tumor cells and isolated rat hepatocytes

    SciTech Connect

    Fry, D.W.; Gewirtz, D.A.; Yalowich, J.C.; Goldman, I.D.

    1983-01-01

    The intracellular synthesis and retention of polygammaglutamyl derivatives of methotrexate and their interactions with H/sub 2/ folate reductase was evaluated. Methotrexate polyglutamates were detected within 15 minutes in hepatocytes exposed to 1 microM methotrexate, and continued to accumulate for at least 60 minutes producing a large transmembrane gradient. These derivatives appeared to be preferentially retained within the cell. In studies with the Ehrlich ascites tumor accumulation of methotrexate polyglutamates was increased over 5-fold by the addition of 5 mM L-glutamine or L-glutamate and exhibited a positive correlation with the extracellular concentration of methotrexate. When Ehrlich ascites tumor cells were exposed to 10 microM methotrexate and 5 mM L-glutamine intracellular polyglutamates were detected within 10 minutes and their levels increased linearly over 4 hours. As these derivatives accumulated, there was a decline in intracellular methotrexate due at least in part to a replacement of methotrexate on H/sub 2/ folate reductase by polyglutamates and subsequent efflux of the previously bound methotrexate from the cell. When polyglutamate derivatives were in excess of the H/sub 2/ folate reductase binding capacity and extracellular methotrexate removed, methotrexate rapidly exited the cell whereas the majority of its metabolites were retained and eventually saturated the major portion of the enzyme. These studies indicate that (1) intracellular methotrexate is rapidly converted to polygammaglutamyl derivatives, (2) these metabolites effectively compete with methotrexate for binding sites on H/sub 2/ folate reductase, (3) these derivatives are retained within the cell more effectively than methotrexate, and (4) vincristine and probenecid may be potentially useful for selectively increasing methotrexate polyglutamates in tumor cells.

  17. Elucidating effects of atmospheric deposition and peat decomposition processes on mercury accumulation rates in a northern Minnesota peatland over last 10,000 cal years

    NASA Astrophysics Data System (ADS)

    Nater, E. A.; Furman, O.; Toner, B. M.; Sebestyen, S. D.; Tfaily, M. M.; Chanton, J.; Fissore, C.; McFarlane, K. J.; Hanson, P. J.; Iversen, C. M.; Kolka, R. K.

    2014-12-01

    Climate change has the potential to affect mercury (Hg), sulfur (S) and carbon (C) stores and cycling in northern peatland ecosystems (NPEs). SPRUCE (Spruce and Peatland Responses Under Climate and Environmental change) is an interdisciplinary study of the effects of elevated temperature and CO2 enrichment on NPEs. Peat cores (0-3.0 m) were collected from 16 large plots located on the S1 peatland (an ombrotrophic bog treed with Picea mariana and Larix laricina) in August, 2012 for baseline characterization before the experiment begins. Peat samples were analyzed at depth increments for total Hg, bulk density, humification indices, and elemental composition. Net Hg accumulation rates over the last 10,000 years were derived from Hg concentrations and peat accumulation rates based on peat depth chronology established using 14C and 13C dating of peat cores. Historic Hg deposition rates are being modeled from pre-industrial deposition rates in S1 scaled by regional lake sediment records. Effects of peatland processes and factors (hydrology, decomposition, redox chemistry, vegetative changes, microtopography) on the biogeochemistry of Hg, S, and other elements are being assessed by comparing observed elemental depth profiles with accumulation profiles predicted solely from atmospheric deposition. We are using principal component analyses and cluster analyses to elucidate relationships between humification indices, peat physical properties, and inorganic and organic geochemistry data to interpret the main processes controlling net Hg accumulation and elemental concentrations in surface and subsurface peat layers. These findings are critical to predicting how climate change will affect future accumulation of Hg as well as existing Hg stores in NPE, and for providing reference baselines for SPRUCE future investigations.

  18. Particle sorting during sediment redistribution processes and the effect on 230Th-normalized mass accumulation rates

    NASA Astrophysics Data System (ADS)

    Marcantonio, Franco; Lyle, Mitchell; Ibrahim, Rami

    2014-08-01

    The 230Th method of determining mass accumulation rates (MARs) assumes that little to no fractionation occurs during sediment redistribution processes at the seafloor. We examine 230Th inventories in radiocarbon-dated multicore sediments from paired winnowed and focused sites at Cocos and Carnegie Ridges, Panama Basin. Radiocarbon-derived sand MARs, which likely represent the vertical rain of particles poorly transported by bottom currents, are similar at each of the paired sites but are different using 230Th normalization. 230Th-normalized MARs are about 60% lower at focused sites and likely underestimate vertical MARs, while the reverse is true for winnowed sites. We hypothesize that size fractionation occurs most frequently at lower current velocities, resulting in the coarse fraction being left behind and primarily the fine 230Th-rich grains being transported downslope. 230Th-normalization works well for recording fine-grained (detrital and opal), but not coarse-grained (carbonate), fluxes in regions that have undergone sediment redistribution.

  19. Sediment accumulation rates in time and space: Paleogene genetic stratigraphic sequences of the northwestern Gulf of Mexico basin

    NASA Astrophysics Data System (ADS)

    Galloway, William E.; Williams, Thomas A.

    1991-10-01

    The Paleogene fill of the northwestern Gulf of Mexico basin consists of eight genetic stratigraphic, sequences bounded by regional marine flooding surfaces. Calculation of sediment accumulation rates along dip profiles through four subbasins shows that regional changes in accumulation rate—and by inference, rate of sediment supply—of fivefold to tenfold repeatedly occurred over time spans of 1 to 3 m.y. Major sequences record episodes of high supply and accommodation-limited accumulation. Periods of declining and low supply were characterized by short-term over-accommodation and consequent transgressive flooding of the basin margin. Within sequences, depositional rates varied with position relative to the contemporaneous shelf margin, with type of depositional system, and between subbasins. Normal patterns of load-induced basin-margin subsidence and creation of accommodation space were modified by perturbation of the crustal stress regime.

  20. Linking the Annual Variation of Snow Radar-derived Accumulation in West Antarctica to Long-term Automatic Weather Station Measurements

    NASA Astrophysics Data System (ADS)

    Feng, B.; Braaten, D. A.; Gogineni, P.; Paden, J. D.; Leuschen, C.; Purdon, K.

    2013-12-01

    Understanding the snow accumulation rate on polar ice sheets is important in assessing mass balance and ice sheet contribution to sea level rise. Measuring annual accumulation on a regional scale and extending back in time several decades has been accomplished using the Center for Remote Sensing of Ice Sheets (CReSIS) Snow Radar on the NASA DC-8 that is part of NASA's Ice-Bridge project. The Snow Radar detects and maps near-surface internal layers in polar firn, operating from 2- 6 GHz and providing a depth resolution of ~4 cm. During November 2011, Snow Radar data were obtained for large areas of West Antarctica, including a flight segment that passed within ~70 km of Byrd Station (80°S, 119°W). Byrd Station has a very long automatic weather station (AWS) record, extending from present to 1980, with 3 relatively brief gaps in the record. The AWS data for Byrd Station were obtained from the Antarctic Meteorological Research Center (AMRC) at the University of Wisconsin. The L1B Snow Radar data products, available from the National Snow and Ice Data Center (NSIDC), were analyzed using layer picking software to obtain the depth of reflectors in the firn that are detected by the radar. These reflectors correspond to annual markers in the firn, and allow annual accumulation to be determined. Using the distance between the reflectors and available density profiles from ice cores, water equivalent accumulation for each annual layer back to 1980 is obtained. We are analyzing spatial variations of accumulation along flight lines, as well as variations in the time series of annual accumulation. We are also analyzing links between annual accumulation and surface weather observations from the Byrd Station AWS. Our analyses of surface weather observations have focused on annual temperature, atmospheric pressure and wind extremes (e.g. 5th and 95th percentiles) and links to annual snow accumulation. We are also examining satellite-derived sea ice extent records for the

  1. Evaluation of C accumulation rates, as determined by 137Cs and 210Pb, for a vegetation gradient in central Alaska

    NASA Astrophysics Data System (ADS)

    Manies, K.; Harden, J. W.; Fuller, C. C.; Turetsky, M. R.

    2012-12-01

    Boreal ecosystems comprise a large portion of the world's soil carbon (C) in large part because the rate of biomass production is greater than the rate of decomposition. To aid in our understanding in the C balance of these systems we need to determine soil C accumulation rates in different ecosystems. Here we use 137Cs and 210Pb chronologies to examine the C accumulation rates of a natural vegetation gradient found within the Bonanza Creek LTER of central Alaska. Five ecosystems, located along a ~300-m transect, were dominated by black spruce, low shrubs, tussock grass, Carex sp., or brown moss. Only the black spruce and shrub site are underlain by permafrost. Three soil cores were taken from each site and analyzed for C content. Depth profiles were also measured for 137Cs and 210Pb, dating the soil horizons, allowing us to estimate C accumulation rates. Average unsupported 210Pb inventories (dpm/cm^2) for each ecosystem type were similar. However, unsupported 210Pb activity was found in the mineral soil horizons for the tussock grass site, suggesting that Pb may be mobile in this system. Estimated dates of formation for each horizon, calculated using both the Constant Flux: Constant Sedimentation (CF:CS) and Constant Rate of Supply (CRS) methods, were usually within +/- 5 years for the past 50 years, but deviated more for older, deeper horizons, likely due to uncertainty in defining the unsupported 210Pb inventory. Recent C accumulation rates varied among ecosystem type, with the black spruce sites having the lowest rate of C accumulation and the other ecosystems (not including the tussock grass site) having approximately the same C accumulation rates. Variability within each ecosystem type, however, was high. The short-term accumulation rates found for these ecosystems (44 - 100 gC/m^2/yr) are within the range of values found in some northern studies (i.e., 40-117 gC/m^2/yr; Turunen et. al, 2004), but higher than those reported by others (i.e., 20-30 gC/m^2/yr

  2. 210Pb mass accumulation rates in the depositional area of the Magra River (Mediterranean Sea, Italy)

    NASA Astrophysics Data System (ADS)

    Delbono, I.; Barsanti, M.; Schirone, A.; Conte, F.; Delfanti, R.

    2016-08-01

    Nine sediment cores were collected between 2009 and 2012 in the inner continental shelf (Mediterranean Sea, Italy) mainly influenced by the Magra River, at water depths ranging from 11 to 64 m. Mass Accumulation Rates (MARs) were calculated through 210Pb analysed by Gamma spectrometry. Three different dating models (single and two-layer CF-CS, CRS) were applied to clay normalised 210Pbxs profiles and 137Cs was used to validate the 210Pb geochronology. The maximum MAR values (>2 g cm-2 yr-1) were found in the region adjacent to the Magra River mouth and outside the Gulf of La Spezia (0.9±0.1 g cm-2 yr-1 at St. 3-C6 and 4-C4). Results from 137Cs/210Pbxs ratios calculated in Surface Mixed Layers (SMLs) evidenced the coastal boundaries of the Magra River depositional area, which is very limited towards south. Differently, in the north-west sector, fine sediments are generally driven by the Ligurian Current and move towards north-west: at the deepest and most distant station from the River mouth, the MAR value is the lowest one in the study area. Few major Magra River floods occurred during the sediment core sampling period. By using the short-lived radioisotope 7Be as a tracer of river floods, a clear 7Be signature of 2009 flood is present at St. 1-SA1C. Finally, by analyzing the clay normalised 210Pbxs profiles, a decrease of its activity dating the years 1999 and 2000 is observed in four cores, corresponding to two major Magra River floods occurring in those years.

  3. Algebraic methods for deriving steady-state rate equations. Practical difficulties with mechanisms that contain repeated rate constants.

    PubMed Central

    Cornish-Bowden, A

    1976-01-01

    Methods of deriving rate equations that rely on repetition of terms for identification of redundant or invalid terms give incorrect results if used with mechanisms in which some rate constants appear more than once. PMID:999635

  4. Accumulate repeat accumulate codes

    NASA Technical Reports Server (NTRS)

    Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

    2004-01-01

    In this paper we propose an innovative channel coding scheme called 'Accumulate Repeat Accumulate codes' (ARA). This class of codes can be viewed as serial turbo-like codes, or as a subclass of Low Density Parity Check (LDPC) codes, thus belief propagation can be used for iterative decoding of ARA codes on a graph. The structure of encoder for this class can be viewed as precoded Repeat Accumulate (RA) code or as precoded Irregular Repeat Accumulate (IRA) code, where simply an accumulator is chosen as a precoder. Thus ARA codes have simple, and very fast encoder structure when they representing LDPC codes. Based on density evolution for LDPC codes through some examples for ARA codes, we show that for maximum variable node degree 5 a minimum bit SNR as low as 0.08 dB from channel capacity for rate 1/2 can be achieved as the block size goes to infinity. Thus based on fixed low maximum variable node degree, its threshold outperforms not only the RA and IRA codes but also the best known LDPC codes with the dame maximum node degree. Furthermore by puncturing the accumulators any desired high rate codes close to code rate 1 can be obtained with thresholds that stay close to the channel capacity thresholds uniformly. Iterative decoding simulation results are provided. The ARA codes also have projected graph or protograph representation that allows for high speed decoder implementation.

  5. Deriving health state utilities for the numerical pain rating scale

    PubMed Central

    2011-01-01

    Background The use of patient reported outcome measures within cost-effectiveness analysis has become commonplace. However, specific measures are required that produce values, referred to as 'utilities', that are capable of generating quality adjusted life years. One such measure - the EQ-5D - has come under criticism due to the inherent limitations of its three-level response scales. In evaluations of chronic pain, the numerical pain rating scale (NPRS) which has eleven levels is routinely used which has a greater measurement range, but which can not be used in cost-effetiveness analyses. This study derived utility values for a series of EQ-5D health states that replace the pain dimensions with the NPRS, thereby allowing a potentially greater range of pain intensities to be captured and included in economic analyses. Methods Interviews were undertaken with 100 member of the general population. Health state valuations were elicited using the time trade-off approach with a ten year time horizon. Additionally, respondents were asked where the EQ-5D response scale descriptors of moderate and extreme pain lay on the 11-point NPRS scale. Results 625 valuations were undertaken across the study sample with the crude mean health state utilities showing a negative non-linear relationship with respect to increasing pain intensity. Relative to a NPRS of zero (NPRS0), the successive pain levels (NPRS1-10) had mean decrements in utility of 0.034, 0.043, 0.061, 0.121, 0.144, 0.252, 0.404, 0.575, 0.771 and 0.793, respectively. When respondents were asked to mark on the NPRS scale the EQ-5D pain descriptors of moderate and extreme pain, the median responses were '4' and '8', respectively. Conclusions These results demonstrate the potential floor effect of the EQ-5D with respect to pain and provide estimates of health reduction associated with pain intensity described by the NPRS. These estimates are in excess of the decrements produced by an application of the EQ-5D scoring tariff

  6. Novel phosphatidylethanolamine derivatives accumulate in circulation in hyperlipidemic ApoE−/− mice and activate platelets via TLR2

    PubMed Central

    Biswas, Sudipta; Xin, Liang; Panigrahi, Soumya; Zimman, Alejandro; Wang, Hua; Yakubenko, Valentin P.; Byzova, Tatiana V.; Salomon, Robert G.

    2016-01-01

    A prothrombotic state and increased platelet reactivity are common in dyslipidemia and oxidative stress. Lipid peroxidation, a major consequence of oxidative stress, generates highly reactive products, including hydroxy-ω-oxoalkenoic acids that modify autologous proteins generating biologically active derivatives. Phosphatidylethanolamine, the second most abundant eukaryotic phospholipid, can also be modified by hydroxy-ω-oxoalkenoic acids. However, the conditions leading to accumulation of such derivatives in circulation and their biological activities remain poorly understood. We now show that carboxyalkylpyrrole-phosphatidylethanolamine derivatives (CAP-PEs) are present in the plasma of hyperlipidemic ApoE−/− mice. CAP-PEs directly bind to TLR2 and induces platelet integrin αIIbβ3 activation and P-selectin expression in a Toll-like receptor 2 (TLR2)-dependent manner. Platelet activation by CAP-PEs includes assembly of TLR2/TLR1 receptor complex, induction of downstream signaling via MyD88/TIRAP, phosphorylation of IRAK4, and subsequent activation of tumor necrosis factor receptor–associated factor 6. This in turn activates the Src family kinases, spleen tyrosine kinase and PLCγ2, and platelet integrins. Murine intravital thrombosis studies demonstrated that CAP-PEs accelerate thrombosis in TLR2-dependent manner and that TLR2 contributes to accelerate thrombosis in mice in the settings of hyperlipidemia. Our study identified the novel end-products of lipid peroxidation, accumulating in circulation in hyperlipidemia and inducing platelet activation by promoting cross-talk between innate immunity and integrin activation signaling pathways. PMID:27015965

  7. Recent rates of carbon accumulation in montane fens ofYosemite National Park, California, U.S.A.

    USGS Publications Warehouse

    Drexler, Judith; Fuller, Christopher C.; Orlando, James; Moore, Peggy E.

    2016-01-01

    Little is known about recent rates of carbon storage in montane peatlands, particularly in the western United States. Here we report on recent rates of carbon accumulation (past 50 to 100 years) in montane groundwater-fed peatlands (fens) of Yosemite National Park in central California, U.S.A. Peat cores were collected at three sites ranging in elevation from 2070 to 2500 m. Core sections were analyzed for bulk density, % organic carbon, and 210Pb activities for dating purposes. Organic carbon densities ranged from 0.026 to 0.065 g C cm-3. Mean vertical accretion rates estimated using210Pb over the 50-year period from ∼1960 to 2011 and the 100-year period from ∼1910 to 2011 were 0.28 (standard deviation = ±0.09) and 0.18 (±-0.04) cm yr-1, respectively. Mean carbon accumulation rates over the 50- and 100-year periods were 95.4 (±25.4) and 74.7 (±17.2) g C m-2 yr-1, respectively. Such rates are similar to recent rates of carbon accumulation in rich fens in western Canada, but more studies are needed to definitively establish both the similarities and differences in peat formation between boreal and temperate montane fens.

  8. [Estimating of decadal accumulation rates of heavy metals in surface rice soils in the Tai Lake region of China].

    PubMed

    Li, Lianqing; Pan, Genxing; Zhang, Pingjiu; Cheng, Jiemin; Zhu, Qiuhua; Qiu, Duosheng

    2002-05-01

    Estimation of decadal accumulation of some heavy metals in surface rice soils from the Tai Lake region, southern Jiangsu Province, China was made by means of calculating the monitoring data and/or analysis data of the archived soil samples. For the last decade, the estimated annual accumulation rate for Cu or Zn, Pb and Cd was 0.3-1 mg.(kg.a)-1, 0.2-1 mg.(kg.a)-1 and 0.3-3 micrograms.(kg.a)-1 respectively, the apparent pollution loading was, therefore, respectively 0.5-1 kg.(hm2.a)-1, 0.5-1.0 kg.(hm2.a)-1, 0.5-3.0 kg.(hm2.a)-1 [symbol: see text] 0.8-10 x 10(-3) kg.(hm2.a)-1. The accumulation rate for the content of available form was shown to be greater than that of total content. The non-point source pollution marked bigger contribution to the total annual loading for the Pb and Cd than the other source pollutions, while the Cd loading was prominently higher than those reported in Europe. These results may indicate that the food safety in this region may be constrained by the soil pollution of these heavy metals at high accumulation rates.

  9. Laboratory Determination of Molybdenum Accumulation Rates as a Measure of Hypoxic Conditions

    EPA Science Inventory

    Redox sensitive metals, such as molybdenum (Mo), are enriched in reducing sediments due to authigenic fixation in anoxic interstitial waters of sediments. This study tested whether the process of fixation and accumulation of Mo in sediments could provide a geochemical indicator o...

  10. Carbon and sediment accumulation in the Everglades (USA) during the past 4000 years: rates, drivers, and sources of error

    USGS Publications Warehouse

    Glaser, Paul H.; Volin, John C.; Givnish, Thomas J.; Hansen, Barbara C. S.; Stricker, Craig A.

    2012-01-01

    Tropical and sub-tropical wetlands are considered to be globally important sources for greenhouse gases but their capacity to store carbon is presumably limited by warm soil temperatures and high rates of decomposition. Unfortunately, these assumptions can be difficult to test across long timescales because the chronology, cumulative mass, and completeness of a sedimentary profile are often difficult to establish. We therefore made a detailed analysis of a core from the principal drainage outlet of the Everglades of South Florida, to assess these problems and determine the factors that could govern carbon accumulation in this large sub-tropical wetland. Accelerator mass spectroscopy dating provided direct evidence for both hard-water and open-system sources of dating errors, whereas cumulative mass varied depending upon the type of method used. Radiocarbon dates of gastropod shells, nevertheless, seemed to provide a reliable chronology for this core once the hard-water error was quantified and subtracted. Long-term accumulation rates were then calculated to be 12.1 g m-2 yr-1 for carbon, which is less than half the average rate reported for northern and tropical peatlands. Moreover, accumulation rates remained slow and relatively steady for both organic and inorganic strata, and the slow rate of sediment accretion ( 0.2 mm yr-1) tracked the correspondingly slow rise in sea level (0.35 mm yr-1 ) reported for South Florida over the past 4000 years. These results suggest that sea level and the local geologic setting may impose long-term constraints on rates of sediment and carbon accumulation in the Everglades and other wetlands.

  11. Using Derivatives to Hedge Interest Rate Risk: A Student Exercise

    ERIC Educational Resources Information Center

    Donaldson, Jeff; Flagg, Donald

    2014-01-01

    In a world of fluctuating asset prices, many firms find the need to hedge in order to avoid or reduce losses. From a gold miner selling gold derivatives to airlines buying oil futures to protect against rising fuel costs, hedging is common practice across many different industries. In this paper, we provide students with a simplified example of a…

  12. Nonpeptidic Lysosomal Modulators Derived from Z-Phe-Ala-Diazomethylketone for Treating Protein Accumulation Diseases

    PubMed Central

    2012-01-01

    Lysosomes are involved in protein turnover and removing misfolded species, and their enzymes have the potential to offset the defect in proteolytic clearance that contributes to the age-related dementia Alzheimer's disease (AD). The weak cathepsin B and L inhibitor Z-Phe-Ala-diazomethylketone (PADK) enhances lysosomal cathepsin levels at low concentrations, thereby eliciting protective clearance of PHF-τ and Aβ42 in the hippocampus and other brain regions. Here, a class of positive modulators is established with compounds decoupled from the cathepsin inhibitory properties. We utilized PADK as a departure point to develop nonpeptidic structures with the hydroxyethyl isostere. The first-in-class modulators SD1002 and SD1003 exhibit improved levels of cathepsin up-regulation but almost complete removal of cathepsin inhibitory properties as compared to PADK. Isomers of the lead compound SD1002 were synthesized, and the modulatory activity was determined to be stereoselective. In addition, the lead compound was tested in transgenic mice with results indicating protection against AD-type protein accumulation pathology. PMID:24900408

  13. Accumulation of plastic-derived chemicals in tissues of seabirds ingesting marine plastics.

    PubMed

    Tanaka, Kosuke; Takada, Hideshige; Yamashita, Rei; Mizukawa, Kaoruko; Fukuwaka, Masa-aki; Watanuki, Yutaka

    2013-04-15

    We analyzed polybrominated diphenyl ethers (PBDEs) in abdominal adipose of oceanic seabirds (short-tailed shearwaters, Puffinus tenuirostris) collected in northern North Pacific Ocean. In 3 of 12 birds, we detected higher-brominated congeners (viz., BDE209 and BDE183), which are not present in the natural prey (pelagic fish) of the birds. The same compounds were present in plastic found in the stomachs of the 3 birds. These data suggested the transfer of plastic-derived chemicals from ingested plastics to the tissues of marine-based organisms.

  14. T1 Relaxation Rate (R1) Indicates Nonlinear Mn Accumulation in Brain Tissue of Welders With Low-Level Exposure

    PubMed Central

    Lee, Eun-Young; Flynn, Michael R.; Du, Guangwei; Lewis, Mechelle M.; Fry, Rebecca; Herring, Amy H.; Van Buren, Eric; Van Buren, Scott; Smeester, Lisa; Kong, Lan; Yang, Qing; Mailman, Richard B.; Huang, Xuemei

    2015-01-01

    Although the essential element manganese (Mn) is neurotoxic at high doses, the effects of lower exposure are unclear. MRI T1-weighted (TIW) imaging has been used to estimate brain Mn exposure via the pallidal index (PI), defined as the T1W intensity ratio in the globus pallidus (GP) versus frontal white matter (FWM). PI may not, however, be sensitive to Mn in GP because Mn also may accumulate in FWM. This study explored: (1) whether T1 relaxation rate (R1) could quantify brain Mn accumulation more sensitively; and (2) the dose-response relationship between estimated Mn exposure and T1 relaxation rate (R1). Thirty-five active welders and 30 controls were studied. Occupational questionnaires were used to estimate hours welding in the past 90 days (HrsW) and lifetime measures of Mn exposure. T1W imaging and T1-measurement were utilized to generate PI and R1 values in brain regions of interest (ROIs). PI did not show a significant association with any measure of Mn and/or welding-related exposure. Conversely, in several ROIs, R1 showed a nonlinear relationship to HrsW, with R1 signal increasing only after a critical exposure was reached. The GP had the greatest rate of Mn accumulation. Welders with higher exposure showed significantly higher R1 compared either with controls or with welders with lower exposure. Our data are additional evidence that Mn accumulation can be assessed more sensitively by R1 than by PI. Moreover, the nonlinear relationship between welding exposure and Mn brain accumulation should be considered in future studies and policies. PMID:25953701

  15. Tapetosomes in Brassica Tapetum Accumulate Endoplasmic Reticulum–Derived Flavonoids and Alkanes for Delivery to the Pollen Surface[W

    PubMed Central

    Hsieh, Kai; Huang, Anthony H.C.

    2007-01-01

    Tapetosomes are abundant organelles in tapetum cells during the active stage of pollen maturation in Brassicaceae species. They possess endoplasmic reticulum (ER)–derived vesicles and oleosin-coated lipid droplets, but their overall composition and function have not been established. In situ localization analyses of developing Brassica napus anthers revealed flavonoids present exclusively in tapetum cells, first in an ER network along with flavonoid-3′-hydroxylase and then in ER-derived tapetosomes. Flavonoids were absent in the cytosol, elaioplasts, vacuoles, and nuclei. Subcellular fractionation of developing anthers localized both flavonoids and alkanes in tapetosomes. Subtapetosome fractionation localized flavonoids in ER-derived vesicles, and alkanes and oleosins in lipid droplets. After tapetum cell death, flavonoids, alkanes, and oleosins were located on mature pollen. In the Arabidopsis thaliana mutants tt12 and tt19 devoid of a flavonoid transporter, flavonoids were present in the cytosol in reduced amounts but absent in tapetosomes and were subsequently located on mature pollen. tt4, tt12, and tt19 pollen was more susceptible than wild-type pollen to UV-B irradiation on subsequent germination. Thus, tapetosomes accumulate ER-derived flavonoids, alkanes, and oleosins for discharge to the pollen surface upon cell death. This tapetosome-originated pollen coat protects the haploidic pollen from UV light damage and water loss and aids water uptake. PMID:17307923

  16. The (137)Cs accumulation by forest-derived products in the Gomel region.

    PubMed

    Bulko, N I; Shabaleva, M A; Kozlov, A K; Tolkacheva, N V; Mashkov, I A

    2014-01-01

    This paper reports basic features of the (137)Cs uptake by forest-derived products in the Gomel region. Even with the soil contamination density of 37 kBq m(-2) the radionuclide contents in 20-30% of mushrooms and berries were found to be higher than the admissible levels. The (137)Cs contamination density of soil, site type and meteorological parameters were observed as the major factors which govern the radiocaesium uptake by mushrooms and berries. The (137)Cs contents in forest-derived products were found to increase directly with the soil contamination density. Bilberries (Vaccinium myrtillus) growing on different site types differed significantly in the (137)Cs content. It was also determined that statistically significant differences in the (137)Cs radioactivity of forest foods growing on different natural sites were governed by the factor designated in the current study by "territorial". The differences are to be accounted for both by forms of the Chernobyl fallout and by the natural and climatic conditions determining variations in the availability of radionuclides in the soil. In dry years the (137)Cs concentrations in some mushroom species were higher than in normal years.

  17. On the Effect of Ramp Rate in Damage Accumulation of the CPV Die-Attach: Preprint

    SciTech Connect

    Bosco, N. S.; Silverman, T. J.; Kurtz, S. R.

    2012-06-01

    It is commonly understood that thermal cycling at high temperature ramp rates may activate unrepresentative failure mechanisms. Increasing the temperature ramp rate of thermal cycling, however, could dramatically reduce the test time required to achieve an equivalent amount of thermal fatigue damage, thereby reducing overall test time. Therefore, the effect of temperature ramp rate on physical damage in the CPV die-attach is investigated. Finite Element Model (FEM) simulations of thermal fatigue and thermal cycling experiments are made to determine if the amount of damage calculated results in a corresponding amount of physical damage measured to the die-attach for a variety of fast temperature ramp rates. Preliminary experimental results are in good agreement with simulations and reinforce the potential of increasing temperature ramp rates. Characterization of the microstructure and resulting fatigue crack in the die-attach suggest a similar failure mechanism across all ramp rates tested.

  18. Transient analysis of acoustically derived pressure and rate data

    SciTech Connect

    Kabir, C.S.; Kuchuk, F.J.; Hasan, A.R.

    1988-09-01

    A pressure-buildup test conducted on a sucker-rod pumping well is often by long-duration wellbore storage. In fact, this distortion could be so severe that even a week's shut-in period may not allow a semilog analysis. A longer shut-in period becomes economically discouraging because of lost production. Low energy and low transmissivity in the reservoir, coupled with increased fluid compressibility, contribute to this long-duration storage phenomenon. One way of reducing the storage effect clearly lies in the simultaneous analysis of downhole pressure and flow rate, estimated from casinghead pressure and rising annular liquid-level measurement made by acoustic well sounding (AWS). Ascertaining the quality of the indirectly measured pressure and rate data constitutes one of the objectives of this study. Several methods exist to translate the AWS measurement to downhole pressure and rate data for the subsequent transient analysis. The authors show that even an empirical hydrodynamic correlation provides satisfactory transient-pressure/flow-rate data for convolution and deconvolution analyses for moderate pumping-liquid columns. When long annular liquid columns are encountered, translating the AWS measurement with a mechanistically based hydrodynamic model appears to be a prudent approach. Interpretation of several transient tests show that automated convolved-type-curve or history matching of field data is a powerful tool for reservoir-parameter (total mobility, skin, fracture half-length, and storage coefficient) estimation. A simple algorithm for computing the Laplace transform of the wellbore pressure for an infinite-conductivity vertically fractured well in an infinite reservoir is developed in this work for a rapid, iterative-type computation used in automated convolved-type-curve analysis.

  19. Observations of historical sea cliff retreat rates exceed long-term estimates derived from cosmogenic 10Be

    NASA Astrophysics Data System (ADS)

    Hurst, Martin D.; Rood, Dylan H.; Ellis, Michael A.; Anderson, Robert S.

    2015-04-01

    Historical observation of coastal retreat are limited to relatively short timescales (< 150 years), during which time humans may have modified the coastal environment. There is growing concern that rates of coastal change may be accelerated in the face of anticipated stormier climates and rising sea level, yet there is little knowledge of rates of coastal change prior to the relatively brief historical records. In order to make predictions about potential future coastal change it is important to establish baseline conditions averaged over longer time periods. Here we present analysis of sea cliff retreat throughout the Holocene averaged for chalk cliffs in south-east England using cosmogenic isotopes. We determine long-term rates of sea cliff erosion from 10Be measured from in-situ flint samples collected from three transects across coastal platforms in East Sussex. A numerical model of 10Be accumulation on an evolving coastal profile allows estimation of cliff retreat rate during the Holocene. The model accounts for variation in 10Be accumulation with tides and sea-level rise, and takes into account platform downwear and topographic shielding by adjacent cliffs. We find that cliff retreat rates during the Holocene were significantly slower (2-6 cm yr-1) than those derived from recent historical observations (15-25 cm yr-1). Modelled accumulation of 10Be requires retreat rates that increase rapidly in recent times, potentially reflecting human modification of the coastal sediment budget through construction of sea defences, flood defenses and aggregate extraction. Therefore knowledge of past human activity at the coastline may be important in anticipating future rates of coastal retreat.

  20. Glucose phosphorylation is not rate limiting for accumulation of glycogen from glucose in perfused livers from fasted rats

    SciTech Connect

    Youn, J.H.; Ader, M.; Bergman, R.N.

    1989-01-05

    Incorporation of Glc and Fru into glycogen was measured in perfused livers from 24-h fasted rats using (6-3H)Glc and (U-14C)Fru. For the initial 20 min, livers were perfused with low Glc (2 mM) to deplete hepatic glycogen and were perfused for the following 30 min with various combinations of Glc and Fru. With constant Fru (2 mM), increasing perfusate Glc increased the relative contribution of Glc carbons to glycogen (7.2 +/- 0.4, 34.9 +/- 2.8, and 59.1 +/- 2.7% at 2, 10, and 20 mM Glc, respectively; n = 5 for each). During perfusion with substrate levels seen during refeeding (10 mM Glc, 1.8 mumol/g/min gluconeogenic flux from 2 mM Fru), Fru provided 54.7 +/- 2.7% of the carbons for glycogen, while Glc provided only 34.9 +/- 2.8%, consistent with in vivo estimations. However, the estimated rate of Glc phosphorylation was at least 1.10 +/- 0.11 mumol/g/min, which exceeded by at least 4-fold the glycogen accumulation rate (0.28 +/- 0.04 mumol of glucose/g/min). The total rate of glucose 6-phosphate supply via Glc phosphorylation and gluconeogenesis (2.9 mumol/g/min) exceeded reported in vivo rates of glycogen accumulation during refeeding. Thus, in perfused livers of 24-h fasted rats there is an apparent redundancy in glucose 6-phosphate supply. These results suggest that the rate-limiting step for hepatic glycogen accumulation during refeeding is located between glucose 6-phosphate and glycogen, rather than at the step of Glc phosphorylation or in the gluconeogenic pathway.

  1. Influence of conversion of penicillin G into a basic derivative on its accumulation and subcellular localization in cultured macrophages

    SciTech Connect

    Renard, C.; Vanderhaeghe, H.J.; Claes, P.J.; Zenebergh, A.; Tulkens, P.M.

    1987-03-01

    beta-Lactam antibiotics do not accumulate in phagocytes, probably because of their acidic character. We therefore synthesized a basic derivative of penicillin G, namely, /sup 14/C-labeled N-(3-dimethylamino-propyl)benzylpenicillinamide (ABP), and studied its uptake and subcellular localization in J774 macrophages compared with that of /sup 14/C-labeled penicillin G. Whereas the intracellular concentration (Ci) of penicillin G remained lower than its extracellular concentration (Ce), ABP reached a Ci/Ce ratio of 4 to 5. Moreover, approximately 50% of intracellular ABP was found associated with lysosomes after isopycnic centrifugation of cell homogenates in isoosmotic Percoll or hyperosmotic sucrose gradients. The behavior of ABP was thus partly consistent with the model of de Duve et al., in which they described the intralysosomal accumulation of weak organic bases in lysosomes. Although ABP is microbiologically inactive, our results show that beta-lactam antibiotics can be driven into cells by appropriate modification. Further efforts therefore may be warranted in the design of active compounds or prodrugs that may prove useful in the chemotherapy of intracellular infections.

  2. The impact of EDTA on the rate of accumulation and root/shoot partitioning of cadmium in mature dwarf sunflowers.

    PubMed

    Meighan, Michelle M; Fenus, Taressa; Karey, Emma; MacNeil, Joseph

    2011-06-01

    In addition to increasing the mobility of metal ions in the soil solution, chelating agents such as EDTA have been reported to alter both the total metal accumulated by plants and its distribution within the plant structures. Here, mature Mini-Sun Hybrid dwarf sunflowers exposed to 300 μM Cd(2+) in hydroponic solution had initial translocation rates of at least 0.12 mmol kg(-1)h(-1) and reached leaf saturation levels within a day when a 3-fold molar excess of EDTA was used. EDTA also promoted cadmium transfer from roots to the shoots. A threefold excess of EDTA increased the translocation factor (TF) 100-fold, resulting in cadmium levels in the leaves of 580 μg g(-1) and extracting 1400 μg plant(-1). When plants were exposed to dissolved cadmium without EDTA, the vast majority of the metal remained bound to the exterior of the root. The initial accumulation could be successfully modeled with a standard biosorption pseudo second-order kinetic equation. Initial accumulation rates ranged from 0.0359 to 0.262 mg g(-1)min(-1). The cadmium binding could be cycled, and did not show evidence of saturation under the experimental conditions employed, suggesting it might be a viable biosorbant for aqueous cadmium.

  3. Metabolically-Derived Human Ventilation Rates: A Revised Approach Based Upon Oxygen Consumption Rates (External Review Draft)

    EPA Science Inventory

    EPA has released a draft report entitled, Metabolically-Derived Human Ventilation Rates: A Revised Approach Based Upon Oxygen Consumption Rates, for independent external peer review and public comment. NCEA published the Exposure Factors Handbook in 1997. This comprehens...

  4. Accumulate-Repeat-Accumulate-Accumulate-Codes

    NASA Technical Reports Server (NTRS)

    Divsalar, Dariush; Dolinar, Sam; Thorpe, Jeremy

    2004-01-01

    Inspired by recently proposed Accumulate-Repeat-Accumulate (ARA) codes [15], in this paper we propose a channel coding scheme called Accumulate-Repeat-Accumulate-Accumulate (ARAA) codes. These codes can be seen as serial turbo-like codes or as a subclass of Low Density Parity Check (LDPC) codes, and they have a projected graph or protograph representation; this allows for a high-speed iterative decoder implementation using belief propagation. An ARAA code can be viewed as a precoded Repeat-and-Accumulate (RA) code with puncturing in concatenation with another accumulator, where simply an accumulator is chosen as the precoder; thus ARAA codes have a very fast encoder structure. Using density evolution on their associated protographs, we find examples of rate-lJ2 ARAA codes with maximum variable node degree 4 for which a minimum bit-SNR as low as 0.21 dB from the channel capacity limit can be achieved as the block size goes to infinity. Such a low threshold cannot be achieved by RA or Irregular RA (IRA) or unstructured irregular LDPC codes with the same constraint on the maximum variable node degree. Furthermore by puncturing the accumulators we can construct families of higher rate ARAA codes with thresholds that stay close to their respective channel capacity thresholds uniformly. Iterative decoding simulation results show comparable performance with the best-known LDPC codes but with very low error floor even at moderate block sizes.

  5. Spatial and temporal variability of snow chemical composition and accumulation rate at Talos Dome site (East Antarctica).

    PubMed

    Caiazzo, Laura; Becagli, Silvia; Frosini, Daniele; Giardi, Fabio; Severi, Mirko; Traversi, Rita; Udisti, Roberto

    2016-04-15

    Five snow pits and five firn cores were sampled during the 2003-2004 Italian Antarctic Campaign at Talos Dome (East Antarctica), where a deep ice core (TALDICE, TALos Dome Ice CorE, 1650m depth) was drilled in 2005-2008 and analyzed for ionic content. Particular attention is spent in applying decontamination procedures to the firn cores, as core sections were stored for approximately 10years before analysis. By considering the snow pit samples to be unperturbed, the comparison with firn core samples from the same location shows that ammonium, nitrate and MSA are affected by storage post-depositional losses. All the other measured ions are confirmed to be irreversibly deposited in the snow layer. The removal of the most external layers (few centimeters) from the firn core sections is proved to be an effective decontamination procedure. High-resolution profiles of seasonal markers (nitrate, sulfate and MSA) allow a reliable stratigraphic dating and a seasonal characterization of the samples. The calculated mean accumulation-rate values range from 70 to 85mmw.e.year(-1), in the period 2003-1973 with small differences between two sectors: 70-74mmw.e.year(-1) in the NNE sector (spanning 2003-1996years) and 81-92mmw.e.year(-1) in the SSW sector (spanning 2003-1980years). This evidence is interpreted as a coupled effect of wind-driven redistribution processes in accumulation/ablation areas. Statistical treatment applied to the concentration values of the snow pits and firn cores samples collected in different points reveals a larger temporal variability than spatial one both in terms of concentration of chemical markers and annual accumulation. The low spatial variability of the accumulation rate and chemical composition measured in the five sites demonstrates that the TALDICE ice core paleo-environmental and paleo-climatic stratigraphies can be considered as reliably representative for the Talos Dome area.

  6. Myotubes from Severely Obese Type 2 Diabetic Subjects Accumulate Less Lipids and Show Higher Lipolytic Rate than Myotubes from Severely Obese Non-Diabetic Subjects

    PubMed Central

    Bakke, Siril S.; Kase, Eili T.; Moro, Cedric; Stensrud, Camilla; Damlien, Lisbeth; Ludahl, Marianne O.; Sandbu, Rune; Solheim, Brita Marie; Rustan, Arild C.; Hjelmesæth, Jøran; Thoresen, G. Hege; Aas, Vigdis

    2015-01-01

    About 80% of patients with type 2 diabetes are classified as overweight. However, only about 1/3 of severely obese subjects have type 2 diabetes. This indicates that several severely obese individuals may possess certain characteristics that protect them against type 2 diabetes. We therefore hypothesized that this apparent paradox could be related to fundamental differences in skeletal muscle lipid handling. Energy metabolism and metabolic flexibility were examined in human myotubes derived from severely obese subjects without (BMI 44±7 kg/m2) and with type 2 diabetes (BMI 43±6 kg/m2). Lower insulin sensitivity was observed in myotubes from severely obese subjects with type 2 diabetes. Lipolysis rate was higher, and oleic acid accumulation, triacylglycerol content, and fatty acid adaptability were lower in myotubes from severely obese subjects with type 2 diabetes compared to severely obese non-diabetic subjects. There were no differences in lipid distribution and mRNA and protein expression of the lipases HSL and ATGL, the lipase cofactor CGI-58, or the lipid droplet proteins PLIN2 and PLIN3. Glucose and oleic acid oxidation were also similar in cells from the two groups. In conclusion, myotubes established from severely obese donors with established type 2 diabetes had lower ability for lipid accumulation and higher lipolysis rate than myotubes from severely obese donors without diabetes. This indicates that a difference in intramyocellular lipid turnover might be fundamental in evolving type 2 diabetes. PMID:25790476

  7. Metabolically Derived human ventilation rates: A revised approach based upon oxygen consumption rates (Final Report) 2009

    EPA Science Inventory

    The purpose of this report is to provide a revised approach for calculating an individual's ventilation rate directly from their oxygen consumption rate. This revised approach will be used to update the ventilation rate information in the Exposure Factors Handbook, which serve as...

  8. Comparing infrared star formation rate indicators with optically derived quantities

    NASA Astrophysics Data System (ADS)

    Young, J. E.; Gronwall, C.; Salzer, J. J.; Rosenberg, J. L.

    2014-09-01

    We examine the UV reprocessing efficiencies of warm dust and polycyclic aromatic hydrocarbons (PAHs) through an analysis of the mid- and far-infrared surface luminosity densities of 85 nearby Hα-selected star-forming galaxies detected by the volume-limited KPNO (Kitt Peak National Observatory) International Spectroscopic Survey (KISS). Because Hα selection is not biased towards continuum-bright objects, the KISS sample spans a wide range in stellar masses (108-1012 M⊙), as well as Hα luminosity (1039-1043 erg s-1), mid-infrared 8.0 μm luminosity (1041-1044 erg s-1), and [Bw - R] colour (-0.1-2.2). We find that mid-infrared PAH emission in the Spitzer InfraRed Array Camera (IRAC) 8.0 μm band correlates with star formation, and that the efficiency with which galaxies reprocess UV energy into PAH emission depends on metallicity. We also find that the relationship between far-infrared luminosity in the Spitzer Multiband Imaging Photometer for Spitzer 24 μm band pass and Hα-measured star formation rate varies from galaxy to galaxy within our sample; we do not observe a metallicity dependence in this relationship. We use optical colours and established mass-to-light relationships to determine stellar masses for the KISS galaxies; we compare these masses to those of nearby galaxies as a confirmation that the volume-limited nature of KISS avoids strong biases. We also examine the relationship between IRAC 3.6 μm luminosity and galaxy stellar mass, and find a colour-dependent correlation between the two.

  9. Photosynthetic rates derived from satellite-based chlorophyll concentration

    SciTech Connect

    Behrenfeld, M.J.; Falkowski, P.G.

    1997-01-01

    We assembled a dataset of C-based productivity measurements to understand the critical variables required for accurate assessment of daily depth-integrated phytoplankton carbon fixation (PP{sub eu}) from measurements of sea surface pigment concentrations (C{sub sat}). From this dataset, we developed a light-dependent, depth-resolved model for carbon fixation (VGPM) that partitions environmental factors affecting primary production into those that influence the relative vertical distribution of primary production (P{sub z}) and those that control the optimal assimilation efficiency of the productivity profile (P{sub opt}{sup B}). The VGPM accounted for 79% of the observed variability in P{sub z} and 86% of the variability in PP{sub eu} by using measured values of P{sub opt}{sup B}. Our results indicate that the accuracy of productivity algorithms in estimating PP{sub eu} is dependent primarily upon the ability to accurately represent variability in P{sub opt}{sup B}. We developed a temperature-dependent P{sub opt}{sup B} model that was used in conjunction with monthly climatological images of C{sub sat}, sea surface temperature, and cloud-corrected estimates of surface irradiance to calculate a global annual phytoplankton carbon fixation (PP{sub annu}) rate of 43.5 Pg C yr{sup {minus}1}. The geographical distribution of PP{sub annu} was distinctly different than results from previous models. Our results illustrate the importance of focusing P{sub opt}{sup B} model development on temporal and spatial, rather than the vertical, variability. 87 refs., 9 figs., 2 tabs.

  10. Rates of plant succession, carbon and nitrogen accumulation in small-scale tundra chronosequences; an implication for climate change

    NASA Astrophysics Data System (ADS)

    Kobayashi, M.; Klaminder, J.

    2011-12-01

    The rate in which plants are able to colonize and build up soil organic carbon (SOC) and nitrogen (N) in soil are crucial in understanding the effect of environmental changes on high latitude ecosystems via plant community. In this presentation we present high-spatial-resolution data of plant colonization and SOC and N accumulation rates occurring on frost boils that are common in many periglacial landscapes. The diameters of each frost boil ranges from 1 to 3 meters. The distribution of plant community across a frost boil can be identified as a gradient of ongoing primary succession. The primary succession is initiated in the centre of the frost boil every time up-frozen soil is deposited on top of the surface. A subsequent lateral mass-movement of newly deposited soil from the centre of the frost boil towards the rim over time causes the surface soil and plant community to become progressively "older" from the centre towards the rim of the frost boil. In the presented work we constrain the age of the soil surface as a function of distance from the centre of the frost boil towards the rim by using lichenometry dating. With this investigation, we achieve soil age gradients (chronosequences) ranging from approximately from 0 to 300 years for meters-scale. We present data from northern Sweden where we have utilized this small-scale variation in soil age to understand how the accumulation rate of SOC and N varies over time in the upper 10 cm of the arctic soil and how the accumulation rates is affected by other ecosystem properties such as temperature, plant diversity, dominant plant functional groups and litter quality. Our key conclusions is that reduced soil frost actions, which is likely to accompany the predicted warming of the Scandinavian arctic, are likely to accelerate the colonization rate of vegetation that will enhance the accumulation of SOC and N. However, one likely side effect of this colonization into previously frost-disturbed system is the decline

  11. Using wavelet analysis to derive seepage rates from thermal records

    NASA Astrophysics Data System (ADS)

    Banzhaf, S.; Onderka, M.; Krein, A.; Scheytt, T.

    2012-04-01

    Luxembourg (BANZHAF et al., 2011) was used. By filtering the temperature raw data with the wavelets, a daily component of temperature variation was isolated and used to calculate daily seepage rates. BANZHAF, S., KREIN, A. & SCHEYTT, T. (2011). Investigative approaches to determine exchange processes in the hyporheic zone of a low permeability riverbank. Hydrogeology Journal 19 (3), pp. 591-601. STALLMAN, R. W. (1965). Steady one-dimensional fluid flow in a semi-infinite porous medium with sinusoidal surface temperature. Journal of Geophysical Research 70 (12). pp. 2821-2827.

  12. TCDD induces dermal accumulation of keratinocyte-derived matrix metalloproteinase-10 in an organotypic model of human skin

    SciTech Connect

    De Abrew, K. Nadira; Thomas-Virnig, Christina L.; Rasmussen, Cathy A.; Bolterstein, Elyse A.; Schlosser, Sandy J.; Allen-Hoffmann, B. Lynn

    2014-05-01

    The epidermis of skin is the first line of defense against the environment. A three dimensional model of human skin was used to investigate tissue-specific phenotypes induced by the environmental contaminant, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Continuous treatment of organotypic cultures of human keratinocytes with TCDD resulted in intracellular spaces between keratinocytes of the basal and immediately suprabasal layers as well as thinning of the basement membrane, in addition to the previously reported hyperkeratinization. These tissue remodeling events were preceded temporally by changes in expression of the extracellular matrix degrading enzyme, matrix metalloproteinase-10 (MMP-10). In organotypic cultures MMP-10 mRNA and protein were highly induced following TCDD treatment. Q-PCR and immunoblot results from TCDD-treated monolayer cultures, as well as indirect immunofluorescence and immunoblot analysis of TCDD-treated organotypic cultures, showed that MMP-10 was specifically contributed by the epidermal keratinocytes but not the dermal fibroblasts. Keratinocyte-derived MMP-10 protein accumulated over time in the dermal compartment of organotypic cultures. TCDD-induced epidermal phenotypes in organotypic cultures were attenuated by the keratinocyte-specific expression of tissue inhibitor of metalloproteinase-1, a known inhibitor of MMP-10. These studies suggest that MMP-10 and possibly other MMP-10-activated MMPs are responsible for the phenotypes exhibited in the basement membrane, the basal keratinocyte layer, and the cornified layer of TCDD-treated organotypic cultures. Our studies reveal a novel mechanism by which the epithelial–stromal microenvironment is altered in a tissue-specific manner thereby inducing structural and functional pathology in the interfollicular epidermis of human skin. - Highlights: • TCDD causes hyperkeratosis and basement membrane changes in a model of human skin. • TCDD induces MMP-10 expression in organotypic cultures

  13. Effect of self-alkalization on nitrite accumulation in a high-rate denitrification system: Performance, microflora and enzymatic activities.

    PubMed

    Li, Wei; Shan, Xiao-Yu; Wang, Zhi-Yao; Lin, Xiao-Yu; Li, Chen-Xu; Cai, Chao-Yang; Abbas, Ghulam; Zhang, Meng; Shen, Li-Dong; Hu, Zhi-Qiang; Zhao, He-Ping; Zheng, Ping

    2016-01-01

    The self-alkalization of denitrifying automatic circulation (DAC) reactor resulted in a large increase of pH up to 9.20 and caused a tremendous accumulation of nitrite up to 451.1 ± 49.0 mgN L(-1) at nitrate loading rate (NLR) from 35 kgN m(-3) d(-1) to 55 kgN m(-3) d(-1). The nitrite accumulation was greatly relieved even at the same NLR once the pH was maintained at 7.6 ± 0.2 in the system. Enzymatic assays indicated that the long-term bacterial exposure to high pH significantly inhibited the activity of copper type nitrite reductase (NirK) rather than the cytochrome cd1 type nitrite reductase (NirS). The terminal restriction fragment length polymorphism (T-RFLP) analysis revealed that the dominant denitrifying bacteria shifted from the NirS-containing Thauear sp. 27 to the NirK-containing Hyphomicrobium nitrativorans strain NL23 during the self-alkalization. The significant nitrite accumulation in the high-rate denitrification system could be therefore, due to the inhibition of Cu-containing NirK by high pH from the self-alkalization. The results suggest that the NirK-containing H. nitrativorans strain NL23 could be an ideal functional bacterium for the conversion of nitrate to nitrite, i.e. denitritation, which could be combined with anaerobic ammonium oxidation (Anammox) to develop a new process for nitrogen removal from wastewater. PMID:26595097

  14. Effect of self-alkalization on nitrite accumulation in a high-rate denitrification system: Performance, microflora and enzymatic activities.

    PubMed

    Li, Wei; Shan, Xiao-Yu; Wang, Zhi-Yao; Lin, Xiao-Yu; Li, Chen-Xu; Cai, Chao-Yang; Abbas, Ghulam; Zhang, Meng; Shen, Li-Dong; Hu, Zhi-Qiang; Zhao, He-Ping; Zheng, Ping

    2016-01-01

    The self-alkalization of denitrifying automatic circulation (DAC) reactor resulted in a large increase of pH up to 9.20 and caused a tremendous accumulation of nitrite up to 451.1 ± 49.0 mgN L(-1) at nitrate loading rate (NLR) from 35 kgN m(-3) d(-1) to 55 kgN m(-3) d(-1). The nitrite accumulation was greatly relieved even at the same NLR once the pH was maintained at 7.6 ± 0.2 in the system. Enzymatic assays indicated that the long-term bacterial exposure to high pH significantly inhibited the activity of copper type nitrite reductase (NirK) rather than the cytochrome cd1 type nitrite reductase (NirS). The terminal restriction fragment length polymorphism (T-RFLP) analysis revealed that the dominant denitrifying bacteria shifted from the NirS-containing Thauear sp. 27 to the NirK-containing Hyphomicrobium nitrativorans strain NL23 during the self-alkalization. The significant nitrite accumulation in the high-rate denitrification system could be therefore, due to the inhibition of Cu-containing NirK by high pH from the self-alkalization. The results suggest that the NirK-containing H. nitrativorans strain NL23 could be an ideal functional bacterium for the conversion of nitrate to nitrite, i.e. denitritation, which could be combined with anaerobic ammonium oxidation (Anammox) to develop a new process for nitrogen removal from wastewater.

  15. Rate of denitrification and the accumulation of intermediates in a denitrifying bioreactor

    NASA Astrophysics Data System (ADS)

    Parsignault, D. R.; Gursky, H.; Kellogg, E. M.; Matilsky, T.; Murray, S.; Schreier, E.; Tananbaum, H.; Giacconi, R.; Brinkman, A. C.

    2012-12-01

    Denitrifying bioreactors (DNBRs) are an emerging mechanism to mitigate the impact of excess reactive nitrogen by harnessing the activity of ubiquitous denitrifying soil microbes. DNBRs fundamentally consist of an organic carbon energy source sufficiently saturated to develop anaerobic conditions and support heterotrophic reduction of nitrate to dinitrogen. Although recent research has well established achievable nitrate removal in DNBRs upwards of 90%, few studies experimentally determine the fate of nitrogen in these systems. This study differentiates between denitrification to inert nitrogen gas, which permanently removes reactive nitrogen from an enriched ecosystem, and transformation of nitrate to another bioavailable form (such as N2O or NOX, powerful greenhouse gases). Previous research has failed to make this distinction and as both are perceived as a reduction in nitrate concentration at the outlet, the utility of DNBRs in reducing downstream reactive nitrogen has not been sufficiently established. In order to quantify the rate of nitrate removal and the products produced, dissolved gas samples are collected from the DNBR with passive diffusion gas samplers while the influent and effluent nitrate concentration and chemical oxygen demand are monitored in real time with spectrometer probes. Nitrate removal is compared with the denitrification rate and the ratio of dinitrogen to nitrous oxide is reported. Denitrification is quantified from the proportion of nitrogen gas products produced from the nitrate pool, indicated by the negative congruence of the regression of 15N enrichment in the nitrate pool and temporal depletion in the gaseous products. The proportion of nitrous oxide to dinitrogen is examined with respect to saturation and redox potential. This research informs the interpretation of previous studies as well as advises the focus of long-term system level monitoring that will provide further information on the design and application of DNBRs to

  16. Mononuclear phagocyte accumulates a stearic acid derivative during differentiation into macrophages. Effects of stearic acid on macrophage differentiation and Mycobacterium tuberculosis control.

    PubMed

    Mosquera-Restrepo, Sergio Fabián; Caro, Ana Cecilia; Peláez-Jaramillo, Carlos Alberto; Rojas, Mauricio

    2016-05-01

    The fatty acid composition of monocytes changes substantially during differentiation into macrophages, increasing the proportion of saturated fatty acids. These changes prompted us to investigate whether fatty acid accumulation in the extracellular milieu could affect the differentiation of bystander mononuclear phagocytes. An esterified fatty acid derivative, stearate, was the only fatty acid that significantly increased in macrophage supernatants, and there were higher levels when cells differentiated in the presence of Mycobacterium tuberculosis H37Rv or purified protein derivative (PPD). Exogenous stearic acid enhanced the expression of HLA-DR and CD64; there was also accumulation of IL-12, TNF-α, IL-6, MIP-1 α and β and a reduction in MCP-1 and the bacterial load. These results suggested that during differentiation, a derivative of stearic acid, which promotes the process as well as the effector mechanisms of phagocytes against the mycobacterium, accumulates in the cell supernatants.

  17. Design and synthesis of a series of piperazine-1-carboxamidine derivatives with antifungal activity resulting from accumulation of endogenous reactive oxygen species.

    PubMed

    François, Isabelle E; Thevissen, Karin; Pellens, Klaartje; Meert, Els M; Heeres, Jan; Freyne, Eddy; Coesemans, Erwin; Viellevoye, Marcel; Deroose, Frederik; Martinez Gonzalez, Sonia; Pastor, Joaquin; Corens, David; Meerpoel, Lieven; Borgers, Marcel; Ausma, Jannie; Dispersyn, Gerrit D; Cammue, Bruno P

    2009-10-01

    In this study, we screened a library of 500 compounds for fungicidal activity via induction of endogenous reactive oxygen species (ROS) accumulation. Structure-activity relationship studies showed that piperazine-1-carboxamidine analogues with large atoms or large side chains substituted on the phenyl group at the R(3) and R(5) positions are characterized by a high ROS accumulation capacity in Candida albicans and a high fungicidal activity. Moreover, we could link the fungicidal mode of action of the piperazine-1-carboxamidine derivatives to the accumulation of endogenous ROS. PMID:19705386

  18. Variations in heavy metal accumulation, growth and yield of rice plants grown at different sewage sludge amendment rates.

    PubMed

    Singh, R P; Agrawal, M

    2010-05-01

    Use of sewage sludge in agriculture is an alternative disposal technique for this waste. The present field study was conducted to assess the suitability of sewage sludge amendment in soil for rice (Oryza sativa L. cv. Pusa sugandha 3) by evaluating the heavy metal accumulation, growth, biomass and yield responses of plants grown at 0, 3, 4.5, 6, 9, 12 kgm(-2) sewage sludge amendment (SSA) rate. Sewage sludge amendment modified the physico-chemical properties of soil, thus increasing the availability of heavy metals in soil and consequently with higher accumulation in plant parts. Root length decreased, whereas shoot length, number of leaves, leaf area and total biomass increased significantly when grown under various SSA rates. Yield of rice increased by 60%, 111%, 125%, 134% and 137% at 3, 4.5, 6, 9 and 12 kgm(-2) SSA, respectively, as compared to those grown in unamended soil. Sewage sludge amendment rates above 4.5 kgm(-2) though increased the yield of rice, but caused risk of food chain contamination as concentrations of Ni and Cd in rice grains were found to be above the Indian safe limits (1.5 mgkg(-1)) of human consumption above 4.5 kgm(-2) SSA and of Pb (2.5 mgkg(-1)) above 6 kgm(-2) SSA. Since aboveground parts of the rice also showed higher concentration than the permissible levels of Ni, Cd and Pb at 4.5 kgm(-2) SSA rate, it cannot be used as fodder. The rice husk may be used as bioresource for energy production. Efforts should be made to treat the effluents from small scale industries before discharge into the sewerage system.

  19. Biogenic silica fluxes and accumulation rates in the Gulf of California

    SciTech Connect

    Thunell, R.C.; Pride, C.J.; Tappa, E. ); Muller-Karger, F.E. )

    1994-04-01

    The Gulf of California, though small in size, plays an important role in the global silica cycle. The seasonal pattern of biogenic silica flux in the gulf is closely related to that of phytoplankton biomass levels and is controlled by changes in weather and hydrographic conditions. The highest opal fluxes ([approximately] 0.35 g[center dot]m[sup [minus]2][center dot]d[sup [minus]1]) occur during winter and spring, and they are comparable to those measured in some of the most productive ecosystems of the world. Approximately 15%-25% of the biogenic silica produced in surface waters is preserved in gulf sediments, a figure significantly higher than the average global ocean preservation rate. However, the flux of opal at 500 m water depth is less than 25% of that being produced at the surface, suggesting that most of the recycling of biogenic silica in the Gulf of California occurs in the upper water column. 28 refs., 3 figs.

  20. Whole genome sequencing of mutation accumulation lines reveals a low mutation rate in the social amoeba Dictyostelium discoideum.

    PubMed

    Saxer, Gerda; Havlak, Paul; Fox, Sara A; Quance, Michael A; Gupta, Sharu; Fofanov, Yuriy; Strassmann, Joan E; Queller, David C

    2012-01-01

    Spontaneous mutations play a central role in evolution. Despite their importance, mutation rates are some of the most elusive parameters to measure in evolutionary biology. The combination of mutation accumulation (MA) experiments and whole-genome sequencing now makes it possible to estimate mutation rates by directly observing new mutations at the molecular level across the whole genome. We performed an MA experiment with the social amoeba Dictyostelium discoideum and sequenced the genomes of three randomly chosen lines using high-throughput sequencing to estimate the spontaneous mutation rate in this model organism. The mitochondrial mutation rate of 6.76×10(-9), with a Poisson confidence interval of 4.1×10(-9) - 9.5×10(-9), per nucleotide per generation is slightly lower than estimates for other taxa. The mutation rate estimate for the nuclear DNA of 2.9×10(-11), with a Poisson confidence interval ranging from 7.4×10(-13) to 1.6×10(-10), is the lowest reported for any eukaryote. These results are consistent with low microsatellite mutation rates previously observed in D. discoideum and low levels of genetic variation observed in wild D. discoideum populations. In addition, D. discoideum has been shown to be quite resistant to DNA damage, which suggests an efficient DNA-repair mechanism that could be an adaptation to life in soil and frequent exposure to intracellular and extracellular mutagenic compounds. The social aspect of the life cycle of D. discoideum and a large portion of the genome under relaxed selection during vegetative growth could also select for a low mutation rate. This hypothesis is supported by a significantly lower mutation rate per cell division in multicellular eukaryotes compared with unicellular eukaryotes. PMID:23056439

  1. Age-Related Accumulation of Somatic Mitochondrial DNA Mutations in Adult-Derived Human iPSCs.

    PubMed

    Kang, Eunju; Wang, Xinjian; Tippner-Hedges, Rebecca; Ma, Hong; Folmes, Clifford D L; Gutierrez, Nuria Marti; Lee, Yeonmi; Van Dyken, Crystal; Ahmed, Riffat; Li, Ying; Koski, Amy; Hayama, Tomonari; Luo, Shiyu; Harding, Cary O; Amato, Paula; Jensen, Jeffrey; Battaglia, David; Lee, David; Wu, Diana; Terzic, Andre; Wolf, Don P; Huang, Taosheng; Mitalipov, Shoukhrat

    2016-05-01

    The genetic integrity of iPSCs is an important consideration for therapeutic application. In this study, we examine the accumulation of somatic mitochondrial genome (mtDNA) mutations in skin fibroblasts, blood, and iPSCs derived from young and elderly subjects (24-72 years). We found that pooled skin and blood mtDNA contained low heteroplasmic point mutations, but a panel of ten individual iPSC lines from each tissue or clonally expanded fibroblasts carried an elevated load of heteroplasmic or homoplasmic mutations, suggesting that somatic mutations randomly arise within individual cells but are not detectable in whole tissues. The frequency of mtDNA defects in iPSCs increased with age, and many mutations were non-synonymous or resided in RNA coding genes and thus can lead to respiratory defects. Our results highlight a need to monitor mtDNA mutations in iPSCs, especially those generated from older patients, and to examine the metabolic status of iPSCs destined for clinical applications. PMID:27151456

  2. SNCA triplication Parkinson's patient's iPSC-derived DA neurons accumulate α-synuclein and are susceptible to oxidative stress.

    PubMed

    Byers, Blake; Cord, Branden; Nguyen, Ha Nam; Schüle, Birgitt; Fenno, Lief; Lee, Patrick C; Deisseroth, Karl; Langston, J William; Pera, Renee Reijo; Palmer, Theo D

    2011-01-01

    Parkinson's disease (PD) is an incurable age-related neurodegenerative disorder affecting both the central and peripheral nervous systems. Although common, the etiology of PD remains poorly understood. Genetic studies infer that the disease results from a complex interaction between genetics and environment and there is growing evidence that PD may represent a constellation of diseases with overlapping yet distinct underlying mechanisms. Novel clinical approaches will require a better understanding of the mechanisms at work within an individual as well as methods to identify the specific array of mechanisms that have contributed to the disease. Induced pluripotent stem cell (iPSC) strategies provide an opportunity to directly study the affected neuronal subtypes in a given patient. Here we report the generation of iPSC-derived midbrain dopaminergic neurons from a patient with a triplication in the α-synuclein gene (SNCA). We observed that the iPSCs readily differentiated into functional neurons. Importantly, the PD-affected line exhibited disease-related phenotypes in culture: accumulation of α-synuclein, inherent overexpression of markers of oxidative stress, and sensitivity to peroxide induced oxidative stress. These findings show that the dominantly-acting PD mutation is intrinsically capable of perturbing normal cell function in culture and confirm that these features reflect, at least in part, a cell autonomous disease process that is independent of exposure to the entire complexity of the diseased brain.

  3. Granulocytic Myeloid-Derived Suppressor Cells Accumulate in Human Placenta and Polarize toward a Th2 Phenotype.

    PubMed

    Köstlin, Natascha; Hofstädter, Kathrin; Ostermeir, Anna-Lena; Spring, Bärbel; Leiber, Anja; Haen, Susanne; Abele, Harald; Bauer, Peter; Pollheimer, Jürgen; Hartl, Dominik; Poets, Christian F; Gille, Christian

    2016-02-01

    Tolerance induction toward the semiallogeneic fetus is crucial to enable a successful pregnancy; its failure is associated with abortion or preterm delivery. Skewing T cell differentiation toward a Th2-dominated phenotype seems to be pivotal in maternal immune adaption, yet underlying mechanisms are incompletely understood. Myeloid-derived suppressor cells (MDSCs) are innate immune cells that mediate T cell suppression and are increased in cord blood of healthy newborns and in peripheral blood of pregnant women. In this study, we demonstrate that granulocytic MDSCs (GR-MDSCs) accumulate in human placenta of healthy pregnancies but are diminished in patients with spontaneous abortions. Placental GR-MDSCs effectively suppressed T cell responses by expression of arginase I and production of reactive oxygen species and were activated at the maternal-fetal interface through interaction with trophoblast cells. Furthermore, GR-MDSCs isolated from placenta polarized CD4(+) T cells toward a Th2 cytokine response. These results highlight a potential role of GR-MDSCs in inducing and maintaining maternal-fetal tolerance and suggest them as a promising target for therapeutic manipulation of pregnancy complications. PMID:26712947

  4. Intermediate filament protein accumulation in motor neurons derived from giant axonal neuropathy iPSCs rescued by restoration of gigaxonin.

    PubMed

    Johnson-Kerner, Bethany L; Ahmad, Faizzan S; Diaz, Alejandro Garcia; Greene, John Palmer; Gray, Steven J; Samulski, Richard Jude; Chung, Wendy K; Van Coster, Rudy; Maertens, Paul; Noggle, Scott A; Henderson, Christopher E; Wichterle, Hynek

    2015-03-01

    Giant axonal neuropathy (GAN) is a progressive neurodegenerative disease caused by autosomal recessive mutations in the GAN gene resulting in a loss of a ubiquitously expressed protein, gigaxonin. Gene replacement therapy is a promising strategy for treatment of the disease; however, the effectiveness and safety of gigaxonin reintroduction have not been tested in human GAN nerve cells. Here we report the derivation of induced pluripotent stem cells (iPSCs) from three GAN patients with different GAN mutations. Motor neurons differentiated from GAN iPSCs exhibit accumulation of neurofilament (NF-L) and peripherin (PRPH) protein and formation of PRPH aggregates, the key pathological phenotypes observed in patients. Introduction of gigaxonin either using a lentiviral vector or as a stable transgene resulted in normalization of NEFL and PRPH levels in GAN neurons and disappearance of PRPH aggregates. Importantly, overexpression of gigaxonin had no adverse effect on survival of GAN neurons, supporting the feasibility of gene replacement therapy. Our findings demonstrate that GAN iPSCs provide a novel model for studying human GAN neuropathologies and for the development and testing of new therapies in relevant cell types.

  5. SNCA Triplication Parkinson's Patient's iPSC-derived DA Neurons Accumulate α-Synuclein and Are Susceptible to Oxidative Stress

    PubMed Central

    Schüle, Birgitt; Fenno, Lief; Lee, Patrick C.; Deisseroth, Karl; Langston, J. William; Pera, Renee Reijo; Palmer, Theo D.

    2011-01-01

    Parkinson's disease (PD) is an incurable age-related neurodegenerative disorder affecting both the central and peripheral nervous systems. Although common, the etiology of PD remains poorly understood. Genetic studies infer that the disease results from a complex interaction between genetics and environment and there is growing evidence that PD may represent a constellation of diseases with overlapping yet distinct underlying mechanisms. Novel clinical approaches will require a better understanding of the mechanisms at work within an individual as well as methods to identify the specific array of mechanisms that have contributed to the disease. Induced pluripotent stem cell (iPSC) strategies provide an opportunity to directly study the affected neuronal subtypes in a given patient. Here we report the generation of iPSC-derived midbrain dopaminergic neurons from a patient with a triplication in the α-synuclein gene (SNCA). We observed that the iPSCs readily differentiated into functional neurons. Importantly, the PD-affected line exhibited disease-related phenotypes in culture: accumulation of α-synuclein, inherent overexpression of markers of oxidative stress, and sensitivity to peroxide induced oxidative stress. These findings show that the dominantly-acting PD mutation is intrinsically capable of perturbing normal cell function in culture and confirm that these features reflect, at least in part, a cell autonomous disease process that is independent of exposure to the entire complexity of the diseased brain. PMID:22110584

  6. SNCA triplication Parkinson's patient's iPSC-derived DA neurons accumulate α-synuclein and are susceptible to oxidative stress.

    PubMed

    Byers, Blake; Cord, Branden; Nguyen, Ha Nam; Schüle, Birgitt; Fenno, Lief; Lee, Patrick C; Deisseroth, Karl; Langston, J William; Pera, Renee Reijo; Palmer, Theo D

    2011-01-01

    Parkinson's disease (PD) is an incurable age-related neurodegenerative disorder affecting both the central and peripheral nervous systems. Although common, the etiology of PD remains poorly understood. Genetic studies infer that the disease results from a complex interaction between genetics and environment and there is growing evidence that PD may represent a constellation of diseases with overlapping yet distinct underlying mechanisms. Novel clinical approaches will require a better understanding of the mechanisms at work within an individual as well as methods to identify the specific array of mechanisms that have contributed to the disease. Induced pluripotent stem cell (iPSC) strategies provide an opportunity to directly study the affected neuronal subtypes in a given patient. Here we report the generation of iPSC-derived midbrain dopaminergic neurons from a patient with a triplication in the α-synuclein gene (SNCA). We observed that the iPSCs readily differentiated into functional neurons. Importantly, the PD-affected line exhibited disease-related phenotypes in culture: accumulation of α-synuclein, inherent overexpression of markers of oxidative stress, and sensitivity to peroxide induced oxidative stress. These findings show that the dominantly-acting PD mutation is intrinsically capable of perturbing normal cell function in culture and confirm that these features reflect, at least in part, a cell autonomous disease process that is independent of exposure to the entire complexity of the diseased brain. PMID:22110584

  7. Amazonian Chemical Weathering Rate Derived from Stony Meteorite Finds at Meridiani Planum on Mars

    NASA Astrophysics Data System (ADS)

    Schröder, C.; Bland, P. A.; Golombek, M. P.; Ashley, J. W.; Warner, N. H.; Grant, J. A.

    2016-08-01

    We used the ferric iron content in stony meteorite finds discovered with the MER Opportunity at Meridiani Planum and constrained their exposure age through related surface features to derive, we believe, the first chemical weathering rate for Mars.

  8. A New Mechanism for the Regulation of Stomatal Aperture Size in Intact Leaves (Accumulation of Mesophyll-Derived Sucrose in the Guard-Cell Wall of Vicia faba).

    PubMed

    Lu, P.; Outlaw Jr, W. H.; Smith, B. G.; Freed, G. A.

    1997-05-01

    At various times after pulse-labeling broad bean (Vicia faba L.) leaflets with 14CO2, whole-leaf pieces and rinsed epidermal peels were harvested and subsequently processed for histochemical analysis. Cells dissected from whole leaf retained apoplastic contents, whereas those from rinsed peels contained only symplastic contents. Sucrose (Suc)-specific radioactivity peaked (111 GBq mol-1) in palisade cells at 20 min. In contrast, the 14C content and Sucspecific radioactivity were very low in guard cells for 20 min, implying little CO2 incorporation; both then peaked at 40 min. The guard-cell apoplast had a high maximum Suc-specific radioactivity (204 GBq mol-1) and a high Suc influx rate (0.05 pmol stoma-1 min-1). These and other comparisons implied the presence of (a) multiple Suc pools in mesophyll cells, (b) a localized mesophyll-apoplast region that exchanges with phloem and stomata, and (c) mesophyll-derived Suc in guard-cell walls sufficient to diminish stomatal opening by approximately 3 [mu]m. Factors expected to enhance Suc accumulation in guard-cell walls are (a) high transpiration rate, which closes stomata, and (b) high apoplastic Suc concentration, which is elevated when mesophyll Suc efflux exceeds translocation. Therefore, multiple physiological factors are integrated in the attenuation of stomatal aperture size by this previously unrecognized mechanism.

  9. A new mechanism for the regulation of stomatal aperture size in intact leaves: Accumulation of mesophyll-derived sucrose in the guard-cell wall of Vicia faba

    SciTech Connect

    Lu, Ping; Outlaw, W.H. Jr.; Smith, B.G.; Freed, G.A.

    1997-05-01

    At various times after pulse-labeling broad bean (Vicia faba L.) leaflets with {sup 14}CO{sub 2}, whole-leaf pieces and rinsed epidermal peels were harvested and subsequently processed for histochemical analysis. Cells dissected from whole leaf retained apoplastic contents, whereas those from rinsed peels contained only symplastic contents. Sucrose (Suc)-specific radioactivity peaked (111 GBq mol{sup -1}) in palisade cells at 20 min. In contrast, the {sup 14}C content and Suc-specific radioactivity were very low in guard cells for 20 min, implying little CO, incorporation; both then peaked at 40 min. The guard-cell apoplast had a high maximum Suc-specific radioactivity (204 GBq mol{sup -1}) and a high Suc influx rate (0.05 pmol stoma{sup -1} min{sup -1}). These and other comparisons implied the presence of (a) multiple Suc pools in mesophyll cells, M a localized mesophyll-apoplast region that exchanges with phloem and stomata, and mesophyll-derived Suc in guard-cell walls sufficient to diminish stomatal opening by approximately 3 pm. Factors expected to enhance Suc accumulation in guard-cell walls are (a) high transpiration rate, which closes stomata, and N high apoplastic Suc concentration, which is elevated when mesophyll Suc efflux exceeds translocation. Therefore, multiple physiological factors are integrated in the attenuation of stomatal aperture size by this previously unrecognized mechanism. 50 refs., 9 figs.

  10. Specific argonautes selectively bind small RNAs derived from potato spindle tuber viroid and attenuate viroid accumulation in vivo.

    PubMed

    Minoia, Sofia; Carbonell, Alberto; Di Serio, Francesco; Gisel, Andreas; Carrington, James C; Navarro, Beatriz; Flores, Ricardo

    2014-10-01

    target and repress viral RNA. Viroids, despite their minimal genomes (non-protein-coding RNAs of only 250 to 400 nt), infect and incite disease in plants. The accumulation in these plants of 21- to 24-nt viroid-derived small RNAs (vd-sRNAs) supports the notion that DCLs also target viroids but does not clarify whether vd-sRNAs activate one or more AGOs. Here, we show that in leaves of Nicotiana benthamiana infected by potato spindle tuber viroid, the endogenous AGO1 and distinct AGOs from Arabidopsis thaliana that were overexpressed were associated with vd-sRNAs displaying the same properties (5'-terminal nucleotide and size) previously established for endogenous and viral small RNAs. Overexpression of AGO1, AGO2, AGO4, and AGO5 attenuated viroid accumulation, supporting their role in antiviroid defense. PMID:25100851

  11. Biomass Accumulation Rates of Amazonian Secondary Forest and Biomass of Old-Growth Forests from Landsat Time Series and GLAS

    NASA Astrophysics Data System (ADS)

    Helmer, E.; Lefsky, M. A.; Roberts, D.

    2009-12-01

    We estimate the age of humid lowland tropical forests in Rondônia, Brazil, from a somewhat densely spaced time series of Landsat images (1975-2003) with an automated procedure, the Threshold Age Mapping Algorithm (TAMA), first described here. We then estimate a landscape-level rate of aboveground woody biomass accumulation of secondary forest by combining forest age mapping with biomass estimates from the Geoscience Laser Altimeter System (GLAS). Though highly variable, the estimated average biomass accumulation rate of 8.4 Mg ha-1 yr-1 agrees well with ground-based studies for young secondary forests in the region. In isolating the lowland forests, we map land cover and general types of old-growth forests with decision tree classification of Landsat imagery and elevation data. We then estimate aboveground live biomass for seven classes of old-growth forest. TAMA is simple, fast, and self-calibrating. By not using between-date band or index differences or trends, it requires neither image normalization nor atmospheric correction. In addition, it uses an approach to map forest cover for the self-calibrations that is novel to forest mapping with satellite imagery; it maps humid secondary forest that is difficult to distinguish from old-growth forest in single-date imagery; it does not assume that forest age equals time since disturbance; and it incorporates Landsat Multispectral Scanner (MSS) imagery. Variations on the work that we present here can be applied to other forested landscapes. Applications that use image time series will be helped by the free distribution of coregistered Landsat imagery, which began in December 2008, and of the Ice Cloud and land Elevation Satellite (ICESat) Vegetation Product, which simplifies the use of GLAS data. Finally, we demonstrate here for the first time how the optical imagery of fine spatial resolution that is viewable on Google Earth provides a new source of reference data for remote sensing applications related to land cover

  12. Exogenous application of pectin-derived oligosaccharides to grape berries modifies anthocyanin accumulation, composition and gene expression.

    PubMed

    Villegas, Daniel; Handford, Michael; Alcalde, José Antonio; Perez-Donoso, Alonso

    2016-07-01

    Anthocyanins are secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway, with functions ranging from skin coloration to protection against pathogens or UV light. Accumulation of these compounds is highly variable depending on genetics, environmental factors and viticultural practices. Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine. The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways. To this end, pre-veraison Cabernet Sauvignon bunches were treated with PDO to subsequently determine total anthocyanin content, the anthocyanin profile (by HPLC-DAD) and gene expression (by qRT-PCR), using Ethrel and water treatments for comparison. The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed. Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins. The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression. A longer term increase in UDP

  13. Exogenous application of pectin-derived oligosaccharides to grape berries modifies anthocyanin accumulation, composition and gene expression.

    PubMed

    Villegas, Daniel; Handford, Michael; Alcalde, José Antonio; Perez-Donoso, Alonso

    2016-07-01

    Anthocyanins are secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway, with functions ranging from skin coloration to protection against pathogens or UV light. Accumulation of these compounds is highly variable depending on genetics, environmental factors and viticultural practices. Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine. The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways. To this end, pre-veraison Cabernet Sauvignon bunches were treated with PDO to subsequently determine total anthocyanin content, the anthocyanin profile (by HPLC-DAD) and gene expression (by qRT-PCR), using Ethrel and water treatments for comparison. The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed. Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins. The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression. A longer term increase in UDP

  14. Accumulation of Cd by the marine sponge Halichondria panicea pallas: Effects upon filtration rate and its relevance for biomonitoring

    SciTech Connect

    Olesen, T.M.E.; Weeks, J.M. )

    1994-05-01

    The marine demosponge Halichondria panicea Pallas, is a cosmopolitan species occurring in coastal waters with varied conditions of light, current, salinity and turbidity. H. panicea has a leuconoid structure and is composed of siliceous spicules and spongin fibers. Sponges are important members of many shallow water marine benthic communities, but comparatively little is known of their trace metal biology. Sponge architecture is constructed around a system of water canals and the physiology of the sponge is largely dependent on the currents of water flowing through their bodies. The volume of water pumped by a sponge is remarkable, ca. 100-1200 ml h[sup [minus]1] g[sup [minus]1]. This large volume of water passing through the body of a sponge means that most cells are in direct contact with the external medium. Many sponges are able to accumulate trace metals and are highly tolerant of such pollutants. This has led to the proposal that a [open quotes]sponge watch[close quotes] program be initiated supplementary to the existing [open quotes]mussel watch[close quotes] program. In view of the large volume of water passing through the bodies of sponges such as H. panicea, the suitability of this species as a biomonitoring organism was further investigated. This study describes the accumulation strategy of the demosponge H. panicea exposed to dissolved cadmium (Cd) and the effect of Cd upon sponge filtration rate.

  15. [Effects of nitrogen application rate on soil nitrate nitrogen accumulation under vegetable-paddy rice rotation system].

    PubMed

    Li, Juan; Zhang, Ming-Qing; Kong, Qing-Bo; Yao, Bao-Quan

    2013-12-01

    A 2-year field experiment of mustard-cabbage-early rice rotation was conducted to investigate the effects of nitrogen application on yield and accumulation of nitrate nitrogen in the soil. The results showed that the applications of 150 kg N x hm(-2) for mustard and cabbage respectively and 90 kg N x hm(-2) for early rice were the best economic application mode, which could increase the net profit by 0.2%-75.6% compared with other application modes. Nitrogen application rates were positively correlated with NO3(-)-N concentration in the soil and in the percolating water. The vegetable-paddy rice rotation decreased the surplus of nitrogen in the soil. The average soil NO3(-)-N concentration was 29.7 mg x kg(-1) under the rotation of mustard-cabbage-early rice, which was only 84.4% of that under the continuous cropping of mustard-cabbage. The average NO3(-)-N concentration in the percolating water under mustard-cabbage-early rice rotation was little different from that in basal soil. Therefore, with the optimum nitrogen application mode, the vegetable-paddy rice rotation could gain the best economic benefit while significantly decrease the accumulation of nitrate nitrogen in the soil to effectively control non-point source pollution of nitrogen from vegetable fields.

  16. Drinking water boosts food intake rate, body mass increase and fat accumulation in migratory blackcaps (Sylvia atricapilla).

    PubMed

    Tsurim, Ido; Sapir, Nir; Belmaker, Jonathan; Shanni, Itai; Izhaki, Ido; Wojciechowski, Michał S; Karasov, William H; Pinshow, Berry

    2008-05-01

    Fat accumulation by blackcaps (Sylvia atricapilla) is a prerequisite for successful migratory flight in the autumn and has recently been determined to be constrained by availability of drinking water. Birds staging in a fruit-rich Pistacia atlantica plantation that had access to water increased their body mass and fat reserves both faster and to a greater extent than birds deprived of water. We conducted a series of laboratory experiments on birds captured during the autumn migration period in which we tested the hypotheses that drinking water increases food use by easing limitations on the birds' dietary choices and, consequently, feeding and food processing rates, and that the availability of drinking water leads to improved digestion and, therefore, to higher apparent metabolizable energy. Blackcaps were trapped in autumn in the Northern Negev Desert, Israel and transferred to individual cages in the laboratory. Birds were provided with P. atlantica fruit and mealworms, and had either free access to water (controls) or were water-deprived. In experiment 1, in which mealworm availability was restricted, water-deprived birds had a fourfold lower fruit and energy intake rates and, consequently, gained less fat and total mass than control birds. Water availability did not affect food metabolizability. In experiment 2, in which mealworms were provided ad libitum, water availability influenced the birds' diet: water-restricted birds ate more mealworms, while control birds consumed mainly P. atlantica fruit. Further, in experiment 2, fat and mass gain did not differ between the two treatment groups. We conclude that water availability may have important consequences for fat accumulation in migrating birds while they fatten at stopover sites, especially when water-rich food is scarce. Restricted water availability may also impede the blackcap's dietary shift from insectivory to frugivory, a shift probably necessary for successful pre-migratory fattening.

  17. Belowground carbon balance and carbon accumulation rate in the successional series of monsoon evergreen broad-leaved forest

    USGS Publications Warehouse

    Zhou, G.; Liu, S.; Tang, X.; Ouyang, X.; Zhang, Dongxiao; Liu, J.; Yan, J.; Zhou, C.; Luo, Y.; Guan, L.; Liu, Yajing

    2006-01-01

    The balance, accumulation rate and temporal dynamics of belowground carbon in the successional series of monsoon evergreen broadleaved forest are obtained in this paper, based on long-term observations to the soil organic matter, input and standing biomass of litter and coarse woody debris, and dissolved organic carbon carried in the hydrological process of subtropical climax forest ecosystem - monsoon evergreen broad-leaved forest, and its two successional forests of natural restoration - coniferous and broad-leaved mixed forest and Pinus massoniana forest, as well as data of root biomass obtained once every five years and respiration measurement of soil, litter and coarse woody debris respiration for 1 year. The major results include: the belowground carbon pools of monsoon evergreen broad-leaved forest, coniferous and broad-leaved mixed forest, and Pinus massoniana forest are 23191 ?? 2538 g ?? m-2, 16889 ?? 1936 g ?? m-2 and 12680 ?? 1854 g ?? m-2, respectively, in 2002. Mean annual carbon accumulation rates of the three forest types during the 24a from 1978 to 2002 are 383 ?? 97 g ?? m-2 ?? a-1, 193 ?? 85 g ?? m-2 ?? a-1 and 213 ?? 86 g ?? m-2 ?? a-1, respectively. The belowground carbon pools in the three forest types keep increasing during the observation period, suggesting that belowground carbon pools are carbon sinks to the atmosphere. There are seasonal variations, namely, they are strong carbon sources from April to June, weak carbon sources from July to September; while they are strong carbon sinks from October to November, weak carbon sinks from December to March. ?? Science in China Press 2006.

  18. Long-term phenological trends, species accumulation rates, aphid traits and climate: five decades of change in migrating aphids.

    PubMed

    Bell, James R; Alderson, Lynda; Izera, Daniela; Kruger, Tracey; Parker, Sue; Pickup, Jon; Shortall, Chris R; Taylor, Mark S; Verrier, Paul; Harrington, Richard

    2015-01-01

    Aphids represent a significant challenge to food production. The Rothamsted Insect Survey (RIS) runs a network of 12·2-m suction-traps throughout the year to collect migrating aphids. In 2014, the RIS celebrated its 50th anniversary. This paper marks that achievement with an extensive spatiotemporal analysis and the provision of the first British annotated checklist of aphids since 1964. Our main aim was to elucidate mechanisms that advance aphid phenology under climate change and explain these using life-history traits. We then highlight emerging pests using accumulation patterns. Linear and nonlinear mixed-effect models estimated the average rate of change per annum and effects of climate on annual counts, first and last flights and length of flight season since 1965. Two climate drivers were used: the accumulated day degrees above 16 °C (ADD16) indicated the potential for migration during the aphid season; the North Atlantic Oscillation (NAO) signalled the severity of the winter before migration took place. All 55 species studied had earlier first flight trends at rate of β = -0·611 ± SE 0·015 days year(-1). Of these species, 49% had earlier last flights, but the average species effect appeared relatively stationary (β = -0·010 ± SE 0·022 days year(-1)). Most species (85%) showed increasing duration of their flight season (β = 0·336 ± SE 0·026 days year(-1)), even though only 54% increased their log annual count (β = 0·002 ± SE <0·001 year(-1)). The ADD16 and NAO were shown to drive patterns in aphid phenology in a spatiotemporal context. Early in the year when the first aphids were migrating, the effect of the winter NAO was highly significant. Further into the year, ADD16 was a strong predictor. Latitude had a near linear effect on first flights, whereas longitude produced a generally less-clear effect on all responses. Aphids that are anholocyclic (permanently parthenogenetic) or are monoecious (non

  19. Long-term phenological trends, species accumulation rates, aphid traits and climate: five decades of change in migrating aphids

    PubMed Central

    Bell, James R; Alderson, Lynda; Izera, Daniela; Kruger, Tracey; Parker, Sue; Pickup, Jon; Shortall, Chris R; Taylor, Mark S; Verrier, Paul; Harrington, Richard

    2015-01-01

    1. Aphids represent a significant challenge to food production. The Rothamsted Insect Survey (RIS) runs a network of 12·2-m suction-traps throughout the year to collect migrating aphids. In 2014, the RIS celebrated its 50th anniversary. This paper marks that achievement with an extensive spatiotemporal analysis and the provision of the first British annotated checklist of aphids since 1964. 2. Our main aim was to elucidate mechanisms that advance aphid phenology under climate change and explain these using life-history traits. We then highlight emerging pests using accumulation patterns. 3. Linear and nonlinear mixed-effect models estimated the average rate of change per annum and effects of climate on annual counts, first and last flights and length of flight season since 1965. Two climate drivers were used: the accumulated day degrees above 16 °C (ADD16) indicated the potential for migration during the aphid season; the North Atlantic Oscillation (NAO) signalled the severity of the winter before migration took place. 4. All 55 species studied had earlier first flight trends at rate of β = −0·611 ± SE 0·015 days year−1. Of these species, 49% had earlier last flights, but the average species effect appeared relatively stationary (β = −0·010 ± SE 0·022 days year−1). Most species (85%) showed increasing duration of their flight season (β = 0·336 ± SE 0·026 days year−1), even though only 54% increased their log annual count (β = 0·002 ± SE <0·001 year−1). 5. The ADD16 and NAO were shown to drive patterns in aphid phenology in a spatiotemporal context. Early in the year when the first aphids were migrating, the effect of the winter NAO was highly significant. Further into the year, ADD16 was a strong predictor. Latitude had a near linear effect on first flights, whereas longitude produced a generally less-clear effect on all responses. Aphids that are anholocyclic (permanently parthenogenetic) or are monoecious (non-host-alternating) were

  20. Steady-State Computation of Constant Rotational Rate Dynamic Stability Derivatives

    NASA Technical Reports Server (NTRS)

    Park, Michael A.; Green, Lawrence L.

    2000-01-01

    Dynamic stability derivatives are essential to predicting the open and closed loop performance, stability, and controllability of aircraft. Computational determination of constant-rate dynamic stability derivatives (derivatives of aircraft forces and moments with respect to constant rotational rates) is currently performed indirectly with finite differencing of multiple time-accurate computational fluid dynamics solutions. Typical time-accurate solutions require excessive amounts of computational time to complete. Formulating Navier-Stokes (N-S) equations in a rotating noninertial reference frame and applying an automatic differentiation tool to the modified code has the potential for directly computing these derivatives with a single, much faster steady-state calculation. The ability to rapidly determine static and dynamic stability derivatives by computational methods can benefit multidisciplinary design methodologies and reduce dependency on wind tunnel measurements. The CFL3D thin-layer N-S computational fluid dynamics code was modified for this study to allow calculations on complex three-dimensional configurations with constant rotation rate components in all three axes. These CFL3D modifications also have direct application to rotorcraft and turbomachinery analyses. The modified CFL3D steady-state calculation is a new capability that showed excellent agreement with results calculated by a similar formulation. The application of automatic differentiation to CFL3D allows the static stability and body-axis rate derivatives to be calculated quickly and exactly.

  1. Processes and rates of sediment and wood accumulation in headwater streams of the Oregon Coast Range, USA

    USGS Publications Warehouse

    May, Christine L.; Gresswell, Robert E.

    2003-01-01

    Channels that have been scoured to bedrock by debris flows provide unique opportunities to calculate the rate of sediment and wood accumulation in low-order streams, to understand the temporal succession of channel morphology following disturbance, and to make inferences about processes associated with input and transport of sediment. Dendrochronology was used to estimate the time since the previous debris flow and the time since the last stand-replacement fire in unlogged basins in the central Coast Range of Oregon. Debris flow activity increased 42 per cent above the background rate in the decades immediately following the last wildfire. Changes in wood and sediment storage were quantified for 13 streams that ranged from 4 to 144 years since the previous debris flow. The volume of wood and sediment in the channel, and the length of channel with exposed bedrock, were strongly correlated with the time since the previous debris flow. Wood increased the storage capacity of the channel and trapped the majority of the sediment in these steep headwater streams. In the absence of wood, channels that have been scoured to bedrock by a debris flow may lack the capacity to store sediment and could persist in a bedrock state for an extended period of time. With an adequate supply of wood, low-order channels have the potential of storing large volumes of sediment in the interval between debris flows and can function as one of the dominant storage reservoirs for sediment in mountainous terrain.

  2. Recent peat accumulation rates in minerotrophic peatlands of the Bay James region, Eastern Canada, inferred by 210Pb and 137Cs radiometric techniques.

    PubMed

    Ali, Adam A; Ghaleb, Bassam; Garneau, Michelle; Asnong, Hans; Loisel, Julie

    2008-10-01

    (210)Pb and (137)Cs dating techniques are used to characterise recent peat accumulation rates of two minerotrophic peatlands located in the La Grande Rivière hydrological watershed, in the James Bay region (Canada). Several cores were collected during the summer 2005 in different parts of the two selected peatlands. These minerotrophic patterned peatlands are presently affected by erosion processes, expressed by progressive mechanical destruction of their pools borders. This erosion process is related to a water table rise induced by a regional increase of humidity since the last century. The main objective of the present paper is to (1) evaluate if (210)Pb and (137)Cs dating techniques can be applied to build accurate chronologies in these environments and (2) detect changes in the peat accumulation rates in regard to this amplification of humidity. In both sites, unsupported (210)Pb shows an exponential decreasing according to the depth. Chronologies inferred from (210)Pb allow to reconstruct peat accumulation rates since ca. 1855 AD. The (137)Cs data displayed evident mobility and diffusion, preventing the establishment of any sustained chronology based on these measurements. In the two sites, peat accumulation rates inferred from (210)Pb chronologies fluctuate between 0.005 and 0.038 g cm(-2) yr(-1). As a result, the rise of the water table during the last decade has not yet affected peat accumulation rates.

  3. Implications of Bishop Tuff zircon U-Pb ages for rates of zircon growth and magma accumulation

    NASA Astrophysics Data System (ADS)

    Reid, M. R.; Schmitt, A. K.

    2012-12-01

    Rates of geologic processes obtained from natural studies rely on accurate geochronologic information. An important benchmark in geochronology as well as a valuable source of insights into the evolution of voluminous explosive eruptions is the >600 km3 Bishop Tuff (BT). A recently determined weighted mean 206Pb/238U date of 767.1±0.9 ka for a BT zircon population [1] is indistinguishable from the recalibrated 40Ar/39Ar sanidine date of 767.4±2.2 ka [2], potentially providing a key intercalibration point between astronomical and radio-isotopic dating approaches. Consequences of these results are linear zircon growth rates of >1×10-14 cm/sec and magma accumulation rates of >200 km3/ka. In contrast, spatially selective SIMS U-Pb dating of BT zircons yielded mean pre-eruption ages of 850 ka [3], a difference that raises questions about the validity of intercalibration between U-Pb and K-Ar dating methods and the history of magma accumulation. We obtained new SIMS analyses of the BT zircons using more spatially and analytically sensitive methods and verifying our accuracy against the TIMS dated Quaternary zircon 61.308A (2.488±0.002 Ma). Analyses were performed on zircon rims and on oriented cross-sections exposed during optical interferometry-calibrated serial sectioning removing the outermost ~31 μm. Sputtering by a 100 nA ion beam versus the normally employed 10-12 nA beam resulted in enhanced radiogenic Pb yields and analytical uncertainties for Quaternary zircon approaching the U-Pb age reproducibility of the primary zircon standard (~1-2 % for AS3). Ages obtained at ~31 μm depth (representing <5% of crystal growth in most cases) average 892±26ka (MSWD=0.29), corroborating previous evidence for residence times of several tens of ka. Rim ages average 781±22 ka (MSWD=0.61), overlapping Ar/Ar determinations of eruption age and corroborating the importance of near-eruption aged zircon growth. Our results confirm the presence of BT zircon domains that predate

  4. Modern sediment characteristics and accumulation rates from the delta front to prodelta of the Yellow River (Huanghe)

    NASA Astrophysics Data System (ADS)

    Zhou, Liangyong; Liu, Jian; Saito, Yoshiki; Gao, Maosheng; Diao, Shaobo; Qiu, Jiandong; Pei, Shaofeng

    2016-08-01

    Since 1976, the main channel of the Yellow River (Huanghe) has been on the east side of the delta complex, and the river has prograded a broad new delta lobe in Laizhou Bay of the Bohai Sea. In 2012, extensive bathymetric and high-resolution seismic profiles were conducted and sediment cores were collected off the new delta lobe. This study examined delta sedimentation and morphology along a profile across the modern subaqueous Yellow River delta and into Laizhou Bay, by analyzing sediment radionuclides (137Cs, 210Pb and 7Be), sedimentary structure, grain-size composition, organic carbon content, and morphological changes between 1976 and 2012. The change in the bathymetric profile, longitudinal to the river's course, reveals subaqueous delta progradation during this period. The subbottom boundary between the new delta lobe sediment and the older seafloor sediment (before the 1976 course shift) was identified in terms of lithology and radionuclide distributions, and recognized as a downlap surface in the seismic record. The accumulation rate of the new delta lobe sediment is estimated to be 5-18.6 cm year-1 on the delta front slope, 2 cm year-1 at the toe of the slope, and 1-2 cm year-1 in the shelf areas of Laizhou Bay. Sediment facies also change offshore, from alternations of gray and brown sediment in the proximal area to gray bioturbated fine sediment in the distal area. Based on 7Be distribution, the shorter-term deposition rate was at least 20 cm year-1 in the delta front.

  5. Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes.

    PubMed

    Eng, George; Lee, Benjamin W; Protas, Lev; Gagliardi, Mark; Brown, Kristy; Kass, Robert S; Keller, Gordon; Robinson, Richard B; Vunjak-Novakovic, Gordana

    2016-01-01

    The therapeutic success of human stem cell-derived cardiomyocytes critically depends on their ability to respond to and integrate with the surrounding electromechanical environment. Currently, the immaturity of human cardiomyocytes derived from stem cells limits their utility for regenerative medicine and biological research. We hypothesize that biomimetic electrical signals regulate the intrinsic beating properties of cardiomyocytes. Here we show that electrical conditioning of human stem cell-derived cardiomyocytes in three-dimensional culture promotes cardiomyocyte maturation, alters their automaticity and enhances connexin expression. Cardiomyocytes adapt their autonomous beating rate to the frequency at which they were stimulated, an effect mediated by the emergence of a rapidly depolarizing cell population, and the expression of hERG. This rate-adaptive behaviour is long lasting and transferable to the surrounding cardiomyocytes. Thus, electrical conditioning may be used to promote cardiomyocyte maturation and establish their automaticity, with implications for cell-based reduction of arrhythmia during heart regeneration. PMID:26785135

  6. Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes

    PubMed Central

    Eng, George; Lee, Benjamin W.; Protas, Lev; Gagliardi, Mark; Brown, Kristy; Kass, Robert S.; Keller, Gordon; Robinson, Richard B.; Vunjak-Novakovic, Gordana

    2016-01-01

    The therapeutic success of human stem cell-derived cardiomyocytes critically depends on their ability to respond to and integrate with the surrounding electromechanical environment. Currently, the immaturity of human cardiomyocytes derived from stem cells limits their utility for regenerative medicine and biological research. We hypothesize that biomimetic electrical signals regulate the intrinsic beating properties of cardiomyocytes. Here we show that electrical conditioning of human stem cell-derived cardiomyocytes in three-dimensional culture promotes cardiomyocyte maturation, alters their automaticity and enhances connexin expression. Cardiomyocytes adapt their autonomous beating rate to the frequency at which they were stimulated, an effect mediated by the emergence of a rapidly depolarizing cell population, and the expression of hERG. This rate-adaptive behaviour is long lasting and transferable to the surrounding cardiomyocytes. Thus, electrical conditioning may be used to promote cardiomyocyte maturation and establish their automaticity, with implications for cell-based reduction of arrhythmia during heart regeneration. PMID:26785135

  7. Accumulation of isochorismate-derived 2,3-dihydroxybenzoic 3-O-beta-D-xyloside in arabidopsis resistance to pathogens and ageing of leaves.

    PubMed

    Bartsch, Michael; Bednarek, Paweł; Vivancos, Pedro D; Schneider, Bernd; von Roepenack-Lahaye, Edda; Foyer, Christine H; Kombrink, Erich; Scheel, Dierk; Parker, Jane E

    2010-08-13

    An intricate network of hormone signals regulates plant development and responses to biotic and abiotic stress. Salicylic acid (SA), derived from the shikimate/isochorismate pathway, is a key hormone in resistance to biotrophic pathogens. Several SA derivatives and associated modifying enzymes have been identified and implicated in the storage and channeling of benzoic acid intermediates or as bioactive molecules. However, the range and modes of action of SA-related metabolites remain elusive. In Arabidopsis, Enhanced Disease Susceptibility 1 (EDS1) promotes SA-dependent and SA-independent responses in resistance against pathogens. Here, we used metabolite profiling of Arabidopsis wild type and eds1 mutant leaf extracts to identify molecules, other than SA, whose accumulation requires EDS1 signaling. Nuclear magnetic resonance and mass spectrometry of isolated and purified compounds revealed 2,3-dihydroxybenzoic acid (2,3-DHBA) as an isochorismate-derived secondary metabolite whose accumulation depends on EDS1 in resistance responses and during ageing of plants. 2,3-DHBA exists predominantly as a xylose-conjugated form (2-hydroxy-3-beta-O-D-xylopyranosyloxy benzoic acid) that is structurally distinct from known SA-glucose conjugates. Analysis of DHBA accumulation profiles in various Arabidopsis mutants suggests an enzymatic route to 2,3-DHBA synthesis that is under the control of EDS1. We propose that components of the EDS1 pathway direct the generation or stabilization of 2,3-DHBA, which as a potentially bioactive molecule is sequestered as a xylose conjugate.

  8. Mauna Loa lava accumulation rates at the Hilo drill site: Formation of lava deltas during a period of declining overall volcanic growth

    USGS Publications Warehouse

    Lipman, P.W.; Moore, J.G.

    1996-01-01

    Accumulation rates for lava flows erupted from Mauna Loa, as sampled in the uppermost 280 m of the Hilo drill hole, vary widely for short time intervals (several thousand years), but overall are broadly similar to those documented elsewhere on this volcano since 100 ka. Thickness variations and accumulation rates for Mauna Loa lavas at the Hilo drill site have been strongly affected by local paleotopography, including funneling and ponding between Mauna Kea and Kilauea. In addition, gentle submerged slopes of Mauna Kea in Hilo Bay have permitted large shoreline displacements by Mauna Loa flows. Ages of eruptive intervals have been determined from published isotopic data and from eustatic sea level curves modified to include the isostatic subsidence of the island of Hawaii at 2.2-2.6 mm/yr. Prior to 10 ka, rates of Mauna Loa lava accumulation at the drill site varied from 0.6 to 4.3 mm/yr for dateable intervals, with an overall rate of 1.8 mm/yr. Major eruptive pulses at about 1.3 and 10 ka, each probably representing a single long-lived eruption based on lack of weathering between flow units, increase the overall accumulation rate to 2.4 mm/yr. The higher rate since 10 ka reflects construction of thick near-shoreline lava deltas as postglacial sea levels rose rapidly. Large lava deltas form only along coastal segments where initially subaerial slopes have been submerged by the combined effects of eustatic sea level rise, isostatic subsidence, or spreading of volcano flanks. Overall accumulation of 239 m of lava at the drill site since 100-120 ka closely balances submergence of the Hilo area, suggesting that processes of coastal lava deposition have been modulated by rise in sea level. The Hilo accumulation rate is slightly higher than average rates of 1-2 mm/yr determined elsewhere along the Mauna Loa coast, based on rates of shoreline coverage and dated sea cliff and fault scarp exposures. Low rates of coastal lava accumulation since 100 ka, near or below the rate

  9. Forest dynamics and tip-up pools drive pulses of high carbon accumulation rates in a tropical peat dome in Borneo (Southeast Asia)

    NASA Astrophysics Data System (ADS)

    Dommain, René; Cobb, Alexander R.; Joosten, Hans; Glaser, Paul H.; Chua, Amy F. L.; Gandois, Laure; Kai, Fuu-Ming; Noren, Anders; Salim, Kamariah A.; Su'ut, N. Salihah H.; Harvey, Charles F.

    2015-04-01

    Peatlands of Southeast Asia store large pools of carbon but the mechanisms of peat accumulation in tropical forests remain to be resolved. Patch dynamics and forest disturbance have seldom been considered as drivers that can amplify or dampen rates of peat accumulation. Here we used a modified piston corer, noninvasive geophysical measurements, and geochemical and paleobotanical techniques to establish the effect of tree fall on carbon accumulation rates in a peat swamp forest dominated by Shorea albida in Brunei (Borneo). Carbon initially accumulated in a mangrove forest at over 300 g C m-2 yr-1 but declined to less than 50 g C m-2 yr-1 with the establishment of a peat swamp forest. A rapid accumulation pulse of 720-960 g C m-2 yr-1 occurred around 1080 years ago as a tip-up pool infilled. Tip-up pools are common in the peatlands of northwest Borneo where windthrow and lightning strikes produce tree falls at a rate of 4 trees ha-1 every decade. A simulation model indicates that tip-up pools, which are formed across the entire forested peat dome, produce local discontinuities in the peat deposit, when peat is removed to create a pool that is rapidly filled with younger material. The resulting discontinuities in peat age at the base and sides of pool deposits obscure linkages between carbon accumulation rates and climate and require new approaches for paleoenvironmental reconstructions. Our results suggest that carbon accumulation in tropical peat swamps may be based on fundamentally different peat-forming processes than those of northern peatlands.

  10. [Effects of nitrogen fertilization rate and planting density on cotton boll biomass and nitrogen accumulation in extremely early maturing cotton region of Northeast China].

    PubMed

    Wang, Zi-Sheng; Wu, Xiao-Dong; Gao, Xiang-Bin; Xu, Min; Shen, Dan; Jin, Lu-Lu; Zhou, Zhi-Guo

    2012-02-01

    Taking cotton cultivars Liaomian 19 and NuCoTN 33B as test materials, a field experiment was conducted to study the effects of nitrogen fertilization rate (0, 240 and 480 kg x hm(-2)) and planting density (75000, 97500 and 120000 plants x hm(-2)) on the boll biomass and nitrogen accumulation in the extremely early maturing cotton region of Northeast China. With the growth and development of cotton, the biomass and nitrogen accumulation of cotton boll, cotton seed, and cotton fiber varied in 'S' shape. Both nitrogen fertilization rate and planting density had significant effects on the dynamic characteristics of boll biomass and nitrogen accumulation, and on the fiber yield and quality. In treatment 240 kg x hm(-2) and 97500 plants x hm(-2), the biomass of single boll, cotton seed and cotton fiber was the maximum, the starting time and ending time of the rapid accumulation period of the biomass and nitrogen were earlier but the duration of the accumulation was shorter, the rapid accumulation speed of the biomass was the maximum, and the distribution indices of the biomass and nitrogen were the lowest in boll shell but the highest in cotton seed and cotton fiber.

  11. Influence of the rate of ethanol production and accumulation on the viability of Saccharomyces cerevisiae in "rapid fermentation".

    PubMed

    Nagodawithana, T W; Steinkraus, K H

    1976-02-01

    Whereas "rapid fermentation" of diluted clover honey (25 degrees Brix) fortified with yeast nutrients using 8 X 10(8) brewers' yeast cells per ml resulted in an ethanol content of 9.5% (wt/vol; 12% vol/vol) in 3 h at 30 C, death rate of the yeast cells during this period was essentially logarithmic. Whereas 6 h was required to reach the same ethanol content at 15 C, the yeast cells retained their viability. Using a lower cell population (6 X 10(7) cells/ml), a level at which the fermentation was no longer "rapid," the yeast cells also retained their viability at 30 C. Ethanol added to the medium was much less lethal than the same or less quantities of ethanol produced by the cell in "rapid fermentation." It was considered possible that ethanol was produced so rapidly at 30 C that it could not diffuse out of the cell as rapidly as it was formed. The hypothesis was postulated that ethanol accumulating in the cell was contributing to the high death rate at 30 C. It was found that the intracellular ethanol concentration reached a level of approximately 2 X 10(11) ethanol molecules/cell in the first 30 min of fermentation at 30 C. At 15 C, with the same cell count, intracellular ethanol concentration reached a level of approximately 4 X 10(10) ethanol molecules/cell and viability remained high. Also, at 30 C with a lower cell population (6 X 10(7) cells/ml), under which conditions fermentation was no longer "rapid," intracellular ethanol concentration reached a similar level (4 X 10(10) molecules ethanol/cell) and the cells retained their viability. Alcohol dehydrogenase (ADH) lost its activity in brewers' yeast under conditions of "rapid fermentation" at 30 C but retained its activity in cells under similar conditions at 15 C. ADH activity was also retained in fermentations at 30 C with cell populations of 6 X 10(7)/ml. It would appear that an intracellular level of about 5 X 10(10) ethanol molecules/cell is normal and that this level does not damage either cell

  12. Contrasting Modern and 10Be- derived erosion rates for the Southern Betic Cordillera, Spain

    NASA Astrophysics Data System (ADS)

    Bellin, N.; Vanacker, V.; Kubik, P.

    2012-04-01

    In Europe, Southeast Spain was identified as one of the regions with major treat of desertification in the context of future land use and climate change. During the last years, significant progress has been made to understand spatial patterns of modern erosion rates in these semi-arid degraded environments. Numerous European projects have contributed to the collection of modern erosion data at different spatial scales for Southeast Spain. However, these data are rarely analysed in the context of long-term changes in vegetation, climate and human occupation. In this paper, we present Modern and Holocene denudation rates for small river basins (1 to 10 km2) located in the Spanish Betic Cordillera. Long-term erosion data were derived from cosmogenic nuclide analyses of river-borne sediment. Modern erosion data were quantified through analysis of sediment deposition volumes behind check dams, and represent average erosion rates over the last 10 to 40 years. Modern erosion rates are surprisingly low (mean erosion rate = 0.048 mm y-1; n=36). They indicate that the steep, sparsely vegetated hillslopes in the Betic Cordillera cannot directly be associated with high erosion rates. 10Be -derived erosion rates integrate over the last 37500 to 3500 years, and are roughly of the same magnitude. They range from 0.013 to 0.243 mm y-1 (mean denudation rate = 0.062 mm y-1 ± 0.054; n=20). Our data suggest that the modern erosion rates are similar to the long-term erosion rates in this area. This result is in contrast with the numerous reports on human-accelerated modern erosion rates for Southeast Spain. Interestingly, our new data on long-term erosion rates show a clear spatial pattern, with higher erosion rates in the Sierra Cabrera and lower erosion rates in Sierra de las Estancias, and Sierra Torrecilla. Preliminary geomorphometric analyses suggest that the spatial variation that we observe in long-term erosion rates is related to the gradient in uplift rates of the Betic

  13. Respiratory rate derived from smartphone-camera-acquired pulse photoplethysmographic signals.

    PubMed

    Lázaro, Jesús; Nam, Yunyoung; Gil, Eduardo; Laguna, Pablo; Chon, Ki H

    2015-11-01

    A method for deriving respiratory rate from smartphone-camera-acquired pulse photoplethysmographic (SCPPG) signal is presented. Our method exploits respiratory information by examining the pulse wave velocity and dispersion from the SCPPG waveform and we term these indices as the pulse width variability (PWV). A method to combine information from several derived respiration signals is also presented and it is used to combine PWV information with other methods such as pulse amplitude variability (PAV), pulse rate variability (PRV), and respiration-induced amplitude and frequency modulations (AM and FM) in SCPPG signals.Evaluation is performed on a database containing SCPPG signals recorded from 30 subjects during controlled respiration experiments at rates from 0.2 to 0.6 Hz with an increment of 0.1 Hz, using three different devices: iPhone 4S, iPod 5, and HTC One M8. Results suggest that spontaneous respiratory rates (0.2-0.4 Hz) can be estimated from SCPPG signals by the PWV- and PRV-based methods with low relative error (median of order 0.5% and interquartile range of order 2.5%). The accuracy can be improved by combining PWV and PRV with other methods such as PAV, AM and/or FM methods. Combination of these methods yielded low relative error for normal respiratory rates, and maintained good performance at higher rates (0.5-0.6 Hz) when using the iPhone 4S or iPod 5 devices.

  14. Respiratory rate derived from smartphone-camera-acquired pulse photoplethysmographic signals.

    PubMed

    Lázaro, Jesús; Nam, Yunyoung; Gil, Eduardo; Laguna, Pablo; Chon, Ki H

    2015-11-01

    A method for deriving respiratory rate from smartphone-camera-acquired pulse photoplethysmographic (SCPPG) signal is presented. Our method exploits respiratory information by examining the pulse wave velocity and dispersion from the SCPPG waveform and we term these indices as the pulse width variability (PWV). A method to combine information from several derived respiration signals is also presented and it is used to combine PWV information with other methods such as pulse amplitude variability (PAV), pulse rate variability (PRV), and respiration-induced amplitude and frequency modulations (AM and FM) in SCPPG signals.Evaluation is performed on a database containing SCPPG signals recorded from 30 subjects during controlled respiration experiments at rates from 0.2 to 0.6 Hz with an increment of 0.1 Hz, using three different devices: iPhone 4S, iPod 5, and HTC One M8. Results suggest that spontaneous respiratory rates (0.2-0.4 Hz) can be estimated from SCPPG signals by the PWV- and PRV-based methods with low relative error (median of order 0.5% and interquartile range of order 2.5%). The accuracy can be improved by combining PWV and PRV with other methods such as PAV, AM and/or FM methods. Combination of these methods yielded low relative error for normal respiratory rates, and maintained good performance at higher rates (0.5-0.6 Hz) when using the iPhone 4S or iPod 5 devices. PMID:26450762

  15. Inhibition of Morganella morganii Histidine Decarboxylase Activity and Histamine Accumulation in Mackerel Muscle Derived from Filipendula ulumaria Extracts.

    PubMed

    Nitta, Yoko; Yasukata, Fumiko; Kitamoto, Noritoshi; Ito, Mikiko; Sakaue, Motoyoshi; Kikuzaki, Hiroe; Ueno, Hiroshi

    2016-03-01

    Filipendula ulmaria, also known as meadowsweet, is an herb; its extract was examined for the prevention of histamine production, primarily that caused by contaminated fish. The efficacy of meadowsweet was assessed using two parameters: inhibition of Morganella morganii histidine decarboxylase (HDC) and inhibition of histamine accumulation in mackerel. Ellagitannins from F. ulmaria (rugosin D, rugosin A methyl ester, tellimagrandin II, and rugosin A) were previously shown to be potent inhibitors of human HDC; and in the present work, these compounds inhibited M. morganii HDC, with half maximal inhibitory concentration values of 1.5, 4.4, 6.1, and 6.8 μM, respectively. Application of the extracts (at 2 wt%) to mackerel meat yielded significantly decreased histamine accumulation compared with treatment with phosphate-buffered saline as a control. Hence, F. ulmaria exhibits inhibitory activity against bacterial HDC and might be effective for preventing food poisoning caused by histamine.

  16. Gas mass derived by infrasound and UV cameras: Implications for mass flow rate

    NASA Astrophysics Data System (ADS)

    Delle Donne, D.; Ripepe, M.; Lacanna, G.; Tamburello, G.; Bitetto, M.; Aiuppa, A.

    2016-10-01

    Mass Flow Rate is one of the most crucial eruption source parameter used to define magnitude of eruption and to quantify the ash dispersal in the atmosphere. However, this parameter is in general difficult to be derived and no valid technique has been developed yet to measure it in real time with sufficient accuracy. Linear acoustics has been applied to infrasonic pressure waves generated by explosive eruptions to indirectly estimate the gas mass erupted and then the mass flow rate. Here, we test on Stromboli volcano (Italy) the performance of such methodology by comparing the acoustic derived results with independent gas mass estimates obtained with UV cameras, and constraining the acoustic source by thermal imagery. We show that different acoustic methods give comparable total gas masses in the 2 to 1425 kg range, which are fully consistent with the gas masses derived by UV cameras and previous direct SO2 measurements. We show that total erupted gas mass, estimated by infrasound is not simply a function of the initial pressure, but rather the full infrasonic waveform should be considered. Thermal imagery provides evidence that infrasound is generated during the entire gas thrust phase. We provide examples to show how total gas masses derived by infrasonic signals can be affected by large uncertainties if duration of the signal is neglected. Only when duration of infrasound is included, the best correlation (0.8) with UV cameras and the 1:1 direct linear proportionality is obtained. Our results open new perspective for remotely derived gas mass and mass flow rates from acoustic signals.

  17. Synergistic infection of two viruses MCMV and SCMV increases the accumulations of both MCMV and MCMV-derived siRNAs in maize

    PubMed Central

    Xia, Zihao; Zhao, Zhenxing; Chen, Ling; Li, Mingjun; Zhou, Tao; Deng, Congliang; Zhou, Qi; Fan, Zaifeng

    2016-01-01

    The co-infection of Maize chlorotic mottle virus (MCMV) and Sugarcane mosaic virus (SCMV) can cause maize lethal necrosis. However, the mechanism underlying the synergistic interaction between these two viruses remains elusive. In this study, we found that the co-infection of MCMV and SCMV increased the accumulation of MCMV. Moreover, the profiles of virus-derived siRNAs (vsiRNAs) from MCMV and SCMV in single- and co-infected maize plants were obtained by high-throughput sequencing. Our data showed that synergistic infection of MCMV and SCMV increased remarkably the accumulation of vsiRNAs from MCMV, which were mainly 22 and 21 nucleotides in length. The single-nucleotide resolution maps of vsiRNAs revealed that vsiRNAs were almost continuously but heterogeneously distributed throughout MCMV and SCMV genomic RNAs, respectively. Moreover, we predicted and annotated dozens of host transcript genes targeted by vsiRNAs. Our results also showed that maize DCLs and several AGOs RNAs were differentially accumulated in maize plants with different treatments (mock, single or double inoculations), which were associated with the accumulation of vsiRNAs. Our findings suggested possible roles of vsiRNAs in the synergistic interaction of MCMV and SCMV in maize plants. PMID:26864602

  18. Rates of post-fire vegetation recovery and fuel accumulation as a function of burn severity and time-since-burn in four western U.S. ecosystems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vegetation recovery and fuel accumulation rates following wildfire are useful measures of ecosystem resilience, yet few studies have quantified these variables over 10 years post-fire. Conventional wisdom is that recovery time to pre-fire condition will be slower as a function of burn severity, as i...

  19. Lunar meteoritic gardening rate derived from in situ LADEE/LDEX measurements

    NASA Astrophysics Data System (ADS)

    Szalay, Jamey R.; Horányi, Mihály

    2016-05-01

    The Lunar Atmosphere and Dust Environment Explorer (LADEE) orbited the Moon for approximately 6 months, taking data with the Lunar Dust Experiment (LDEX). LDEX was uniquely equipped to characterize the current rate of lunar impact gardening as it measured the very particles taking part in this process. By deriving an average lunar dust density distribution, we calculate the rate at which exospheric dust rains back down onto the lunar surface. Near the equatorial plane, we find that approximately 40 μm/Myr of lunar regolith, with a cumulative size distribution index of 2.7, is redistributed due to meteoritic bombardment, a process which occurs predominantly on the lunar apex hemisphere.

  20. Derivation of the chemical-equilibrium rate coefficient using scattering theory

    NASA Technical Reports Server (NTRS)

    Mickens, R. E.

    1977-01-01

    Scattering theory is applied to derive the equilibrium rate coefficient for a general homogeneous chemical reaction involving ideal gases. The reaction rate is expressed in terms of the product of a number of normalized momentum distribution functions, the product of the number of molecules with a given internal energy state, and the spin-averaged T-matrix elements. An expression for momentum distribution at equilibrium for an arbitrary molecule is presented, and the number of molecules with a given internal-energy state is represented by an expression which includes the partition function.

  1. Roles of rhizosphere and root-derived organic acids in Cd accumulation by two hot pepper cultivars.

    PubMed

    Xin, Junliang; Huang, Baifei; Dai, Hongwen; Zhou, Wenjing; Yi, Yumei; Peng, Lijing

    2015-04-01

    Cultivars of hot pepper (Capsicum annuum L.) have different abilities to accumulate Cd in their fruits. Previously, we suggested that low-Cd cultivars take up more Cd, but can better prevent the Cd translocation from roots to aerial parts. However, the mechanisms involved in those processes are still unclear. In this study, we explored the roles of rhizosphere soil Cd fractions and root secretions of low molecular weight organic acids in the uptake, translocation, and accumulation of Cd in a low-Cd and high-Cd cultivar. The results showed that there was no significant difference in exchangeable Cd between rhizosphere soils of the two cultivars, which might be related to their similar root's Cd uptake ability. The total content of low molecular weight organic acids released from roots of the low-Cd cultivar was almost equal to that released from roots of the high-Cd cultivar at the same Cd level; however, the composition of low molecular weight organic acids were determined by cultivars and Cd exposure levels. In the higher Cd (10 μM) treatment, the roots of the low-Cd cultivar excreted significantly less tartaric acid and more oxalic and acetic acids than those of the high-Cd cultivar. Additionally, there was no difference in the concentration of citric or succinic acid between the two cultivars. These results indicate that some kinds of low molecular weight organic acids efflux from hot pepper roots played an important role in the difference of Cd accumulation between low- and high-Cd cultivars.

  2. A 9000 year perspective on carbon accumulation rates under changing hydro-climate and vegetation conditions in a mountain peatland, northern Carpathians, Romania

    NASA Astrophysics Data System (ADS)

    Feurdean, Angelica; Panait, Andrei; Gałka, Mariusz; Diaconu, Andrei; Hutchinson, Simon; Mulch, Andreas; Tantau, Ioan; Hickler, Thomas

    2016-04-01

    Peatlands, in particular ombrogenous bogs, which entirely depend on water from precipitation, are sensitive to changes in the balance between precipitation and evapotranspiration; and therefore highly suitable for hydro-climatological reconstruction. Peatlands also represent a large carbon pool in the terrestrial biosphere. However, little is known about the C sequestration processes in mountain peatlands under various competing drivers of change (climate, vegetation, fire). We applied a multi-proxy approach (bulk density, loss on ignition, total organic carbon, testate amoebae, δ13C in Sphagnum, plant macrofossils, pollen and charcoal) to a peat sequence from a mountain ombrogenous bog (Tǎul Muced) to explore how changes in hydro-climate conditions, peat plant composition and fire have affected long-term physical peat properties and the rate of carbon accumulation over the last 9000 years. Carbon accumulation at this site ranged from 7 to 105 g C cm2 yr1 (mean 23 ± 14 g C cm2 yr_1). We found that high moisture availability (P-E) as inferred from testate amoebae and δ13C values in Sphagnum increased the carbon sink capacity of peatland. The strength of the relationship between the rate of carbon accumulation and climate appears particularly evident over the last millennium when high C accumulation rates correlated with the warm and wet conditions of the Medieval Climate Anomaly and lower C accumulation rates with the dry conditions of the Little Ice Age. We also found a significant positive correlation between the rate of C accumulation and changes in vegetation; rates were lowest (17 g C cm2 yr_1), during periods of mixed Sphagnum (primarily S. magellanicum and S. angustifolium) and vascular plant (Cyperaceae, Eriophorum vaginatum) growth and increased (31 g C cm2 yr_1) during the accumulation of Sphagnum peat, regardless the dominant Sphagnum species. We did not find indication of peatland fire during the investigated interval. Our study represents one of the

  3. Lipid peroxidation-derived 4-hydroxynonenal-modified proteins accumulate in human facial skin fibroblasts during ageing in vitro.

    PubMed

    Jørgensen, Peter; Milkovic, Lidija; Zarkovic, Neven; Waeg, Georg; Rattan, Suresh I S

    2014-02-01

    The reactive aldehyde, 4-hydroxynonenal (HNE), is recognized as a product of lipid peroxidation, which binds to macromolecules, in particular proteins. HNE-modified proteins (HNE-MP) have been shown to accumulate during ageing, generally by using polyclonal antibodies, which increase the possibility of detecting false positives. Therefore, we have used a genuine monoclonal antibody specific for HNE-His adducts of proteins/peptides, which were revealed by immunoblotting method for whole-cell HNE-MP measurements in serially passaged human facial skin fibroblasts undergoing ageing in vitro. There was a significant increase in the levels of HNE-MP in serially passaged cells approaching a near senescent state at high passage level (P-61), as compared with low passage level (P-11) young and middle-aged (P-27) cells. However, if the cells were analyzed soon after re-initiation from the frozen samples with little further passaging, the amount of HNE-MP was low even in relatively high passage level (P-37) cells, which is an indication of selective elimination of cells with high molecular damage during the process of thawing and re-initiation in culture. This pilot study on normal human facial skin fibroblasts shows that HNE-MP detection by monoclonal antibody-based dot blot method can be used as a marker for age-related accumulation of lipid peroxidative molecular damage, and could be useful for testing and monitoring the effects of potential skin care products on ageing parameters.

  4. Seasonal variation of meteorological variables and recent surface ablation / accumulation rates on Davies Dome and Whisky Glacier, James Ross Island, Antarctica

    NASA Astrophysics Data System (ADS)

    Láska, K.; Nývlt, D.; Engel, Z.; Budík, L.

    2012-04-01

    In this study, surface mass balance data of two glaciers on James Ross Island, Antarctica, and its spatial and temporal variations are evaluated using snow ablation stakes, ground-penetrating radar, and dGPS measurements. The investigated glaciers are located on the Ulu Peninsula, northern part of James Ross Island. Davies Dome is an ice dome, which originates on the surface of a flat volcanic mesa at elevations >400 m a.s.l. and terminates with a single 700 m wide outlet in the Whisky Bay. Davies Dome has an area of ~6.5 km2 and lies in the altitude range of 0-514 m a.s.l. Whisky Glacier is a cold-based land-terminating valley glacier surrounded by an extensive moraine ridges made of debris-covered ice. The glacier has an area of ~2.4 km2 and lies in the altitude range of 215-520 m a.s.l. Within several summer austral summers, extensive field programme were carried out on both glaciers including the operation of two automatic weather stations, field mapping and mass balance measurements. Each station was equipped with albedometer CM7B (Kipp-Zonen, Netherlands), air temperature and humidity sensor EMS33 (EMS, Czech Republic), propeller anemometer 05103 (Young, USA), and snow depth sensors (Judd, USA). In the period 2009-2011, high seasonal and interdiurnal variability of incoming solar radiation and near-surface air temperature was found as a result of changes in the circulation patterns and synoptic-scale weather systems moving in the Circumpolar Trough. High ablation and accumulation rates were recorded mainly in the spring and summer seasons (October-February), while negligible changes were found in winter (May-September). The effects of positive degree-day temperatures on the surface ablation rates were examined using a linear regression model. In this approach, near-surface air temperature maps on the glacier surfaces were derived from digital elevation model according to actual temperature lapse rates. Mass balance investigations started in 2006 on Davies

  5. Changes in salivary flow rate following Chlorella-derived multicomponent supplementation.

    PubMed

    Otsuki, Takeshi; Shimizu, Kazuhiro; Zempo-Miyaki, Asako; Maeda, Seiji

    2016-07-01

    Decreases in saliva secretion compromise food mastication and swallowing, reduce mucosal immune function, and increase the risk for oral diseases like dental caries. Chlorella is a green alga that contains a variety of nutrients including amino acids, vitamins, and minerals. In our previous study, Chlorella-derived multicomponent supplementation did not affect salivary flow rates in healthy young individuals, but Chlorella-derived supplementation attenuated a decrease in saliva secretion that was observed during a kendo training camp. Hence, we hypothesized that Chlorella-derived supplementation increases saliva secretion in individuals with lower rates of saliva flow. Sixty-four subjects took Chlorella-derived tablets for four weeks. Before and after supplementation, saliva samples were collected by chewing cotton. In the complete study group, there was no difference in saliva production before and after supplementation (1.91 ± 0.11 ml/min before vs 2.01 ± 0.12 ml/min after). Analysis of subgroups based on saliva production before supplementation found an increase in saliva secretion in the lower saliva flow group (1.18 ± 0.06 vs 1.38 ± 0.08 ml/min), but no change in the higher saliva flow group (2.63 ± 0.11 vs 2.64 ± 0.15 ml/min). These results suggest that Chlorella-derived multicomponent supplementation increases saliva production in individuals with lower levels of saliva secretion. PMID:27499578

  6. Empirical mass-loss rates for 25 O and early B stars, derived from Copernicus observations

    NASA Technical Reports Server (NTRS)

    Gathier, R.; Lamers, H. J. G. L. M.; Snow, T. P.

    1981-01-01

    Ultraviolet line profiles are fitted with theoretical line profiles in the cases of 25 stars covering a spectral type range from O4 to B1, including all luminosity classes. Ion column densities are compared for the determination of wind ionization, and it is found that the O VI/N V ratio is dependent on the mean density of the wind and not on effective temperature value, while the Si IV/N V ratio is temperature-dependent. The column densities are used to derive a mass-loss rate parameter that is empirically correlated against the mass-loss rate by means of standard stars with well-determined rates from IR or radio data. The empirical mass-loss rates obtained are compared with those derived by others and found to vary by as much as a factor of 10, which is shown to be due to uncertainties or errors in the ionization fractions of models used for wind ionization balance prediction.

  7. Impairment of aldehyde dehydrogenase 2 increases accumulation of acetaldehyde-derived DNA damage in the esophagus after ethanol ingestion

    PubMed Central

    Yukawa, Yoshiyuki; Ohashi, Shinya; Amanuma, Yusuke; Nakai, Yukie; Tsurumaki, Mihoko; Kikuchi, Osamu; Miyamoto, Shin’ichi; Oyama, Tsunehiro; Kawamoto, Toshihiro; Chiba, Tsutomu; Matsuda, Tomonari; Muto, Manabu

    2014-01-01

    Ethanol and its metabolite, acetaldehyde, are the definite carcinogens for esophageal squamous cell carcinoma (ESCC), and reduced catalytic activity of aldehyde dehydrogenase 2 (ALDH2), which detoxifies acetaldehyde, increases the risk for ESCC. However, it remains unknown whether the ALDH2 genotype influences the level of acetaldehyde-derived DNA damage in the esophagus after ethanol ingestion. In the present study, we administered ethanol orally or intraperitoneally to Aldh2-knockout and control mice, and we quantified the level of acetaldehyde-derived DNA damage, especially N2-ethylidene-2’-deoxyguanosine (N2-ethylidene-dG), in the esophagus. In the model of oral ethanol administration, the esophageal N2-ethylidene-dG level was significantly higher in Aldh2-knockout mice compared with control mice. Similarly, in the model of intraperitoneal ethanol administration, in which the esophagus is not exposed directly to the alcohol solution, the esophageal N2-ethylidene-dG level was also elevated in Aldh2-knockout mice. This result indicates that circulating ethanol-derived acetaldehyde causes esophageal DNA damage, and that the extent of damage is influenced by knockout of Aldh2. Taken together, our findings strongly suggest the importance of acetaldehyde-derived DNA damage which is induced in the esophagus of individuals with ALDH2 gene impairment. This provides a physiological basis for understanding alcohol-related esophageal carcinogenesis. PMID:24959382

  8. Impairment of aldehyde dehydrogenase 2 increases accumulation of acetaldehyde-derived DNA damage in the esophagus after ethanol ingestion.

    PubMed

    Yukawa, Yoshiyuki; Ohashi, Shinya; Amanuma, Yusuke; Nakai, Yukie; Tsurumaki, Mihoko; Kikuchi, Osamu; Miyamoto, Shin'ichi; Oyama, Tsunehiro; Kawamoto, Toshihiro; Chiba, Tsutomu; Matsuda, Tomonari; Muto, Manabu

    2014-01-01

    Ethanol and its metabolite, acetaldehyde, are the definite carcinogens for esophageal squamous cell carcinoma (ESCC), and reduced catalytic activity of aldehyde dehydrogenase 2 (ALDH2), which detoxifies acetaldehyde, increases the risk for ESCC. However, it remains unknown whether the ALDH2 genotype influences the level of acetaldehyde-derived DNA damage in the esophagus after ethanol ingestion. In the present study, we administered ethanol orally or intraperitoneally to Aldh2-knockout and control mice, and we quantified the level of acetaldehyde-derived DNA damage, especially N(2) -ethylidene-2'-deoxyguanosine (N(2) -ethylidene-dG), in the esophagus. In the model of oral ethanol administration, the esophageal N(2) -ethylidene-dG level was significantly higher in Aldh2-knockout mice compared with control mice. Similarly, in the model of intraperitoneal ethanol administration, in which the esophagus is not exposed directly to the alcohol solution, the esophageal N(2) -ethylidene-dG level was also elevated in Aldh2-knockout mice. This result indicates that circulating ethanol-derived acetaldehyde causes esophageal DNA damage, and that the extent of damage is influenced by knockout of Aldh2. Taken together, our findings strongly suggest the importance of acetaldehyde-derived DNA damage which is induced in the esophagus of individuals with ALDH2 gene impairment. This provides a physiological basis for understanding alcohol-related esophageal carcinogenesis. PMID:24959382

  9. The effect of interest rate derivative transactions on debt savings for not-for-profit health systems.

    PubMed

    Venkataramani, Prakash; Johnson, Tricia; O'Neil, Patricia; Poindexter, Victoria; Rooney, Jeffrey

    2006-01-01

    The utilization of interest rate derivative instruments in US for-profit companies has grown exponentially since the early 1980s. International Swaps and Derivatives Association, Inc. (ISDA), reported that the amount of outstanding standard swaps grew by 25 percent during the first six months of 2003. The growth rate of all interest rate derivatives, which includes single-currency interest rate swaps, cross-currency interest rate swaps, and interest rate options, grew by 24 percent during the same period. The total outstanding amount of interest rate derivatives now totals $123.9 trillion compared to $99.9 trillion at the end of 2002 (Dodd, 2003). This explosion in usage is a testament to the efficacy and flexibility of the instruments and the increased appreciation by financial managers of the importance of financial risk management in a volatile interest rate environment. PMID:19175238

  10. Doppler-derived myocardial systolic strain rate is a strong index of left ventricular contractility

    NASA Technical Reports Server (NTRS)

    Greenberg, Neil L.; Firstenberg, Michael S.; Castro, Peter L.; Main, Michael; Travaglini, Agnese; Odabashian, Jill A.; Drinko, Jeanne K.; Rodriguez, L. Leonardo; Thomas, James D.; Garcia, Mario J.

    2002-01-01

    BACKGROUND: Myocardial fiber strain is directly related to left ventricular (LV) contractility. Strain rate can be estimated as the spatial derivative of velocities (dV/ds) obtained by tissue Doppler echocardiography (TDE). The purposes of the study were (1) to determine whether TDE-derived strain rate may be used as a noninvasive, quantitative index of contractility and (2) to compare the relative accuracy of systolic strain rate against TDE velocities alone. METHODS AND RESULTS: TDE color M-mode images of the interventricular septum were recorded from the apical 4-chamber view in 7 closed-chest anesthetized mongrel dogs during 5 different inotropic stages. Simultaneous LV volume and pressure were obtained with a combined conductance-high-fidelity pressure catheter. Peak elastance (Emax) was determined as the slope of end-systolic pressure-volume relationships during caval occlusion and was used as the gold standard of LV contractility. Peak systolic TDE myocardial velocities (Sm) and peak (epsilon'(p)) and mean (epsilon'(m)) strain rates obtained at the basal septum were compared against Emax by linear regression. Emax as well as TDE systolic indices increased during inotropic stimulation with dobutamine and decreased with the infusion of esmolol. A stronger association was found between Emax and epsilon'(p) (r=0.94, P<0.01, y=0.29x+0.46) and epsilon'(m) (r=0.88, P<0.01) than for Sm (r=0.75, P<0.01). CONCLUSIONS: TDE-derived epsilon'(p) and epsilon'(m) are strong noninvasive indices of LV contractility. These indices appear to be more reliable than S(m), perhaps by eliminating translational artifact.

  11. Predominant anthropogenic sources and rates of atmospheric mercury accumulation in southern Ontario recorded by peat cores from three bogs: comparison with natural "background" values (past 8000 years).

    PubMed

    Givelet, Nicolas; Roos-Barraclough, Fiona; Shotyk, William

    2003-12-01

    Peat cores from three bogs in southern Ontario provide a complete, quantitative record of net rates of atmospheric Hg accumulation since pre-industrial times. For comparison with modern values, a peat core extending back 8000 years was used to quantify the natural variations in Hg fluxes for this region, and their dependence on climatic change and land use history. The net mercury accumulation rates were separated into "natural" and "excess" components by comparing the Hg/Br ratios of modern samples with the long-term, pre-anthropogenic average Hg/Br. The average background mercury accumulation rate during the pre-anthropogenic period (from 5700 years BC to 1470 AD) was 1.4 +/- 1.0 microg m(-2) per year (n = 197). The beginning of Hg contamination from anthropogenic sources dates from AD 1475 at the Luther Bog, corresponding to biomass burning for agricultural activities by Native North Americans. During the late 17th and 18th centuries, deposition of anthropogenic Hg was at least equal to that of Hg from natural sources. Anthropogenic inputs of Hg to the bogs have dominated continuously since the beginning of the 19th century. The maximum Hg accumulation rates decrease in the order Sifton Bog, in the City of London, Ontario (141 microg Hg m(-2) per year), Luther Bog in an agricultural region (89 microg Hg m(-2) per year), and Spruce Bog which is in a comparatively remote, forested region (54 microg Hg m(-2) per year). Accurate age dating of recent peat samples using the bomb pulse curve of 14C shows that the maximum rate of atmospheric Hg accumulation occurred during AD 1956 and 1959 at all sites. In these (modern) samples, the Hg concentration profiles resemble those of Pb, an element which is known to be immobile in peat bogs. The correlation between these two metals, together with sulfur, suggests that the predominant anthropogenic source of Hg (and Pb) was coal burning. While Hg accumulation rates have gone into strong decline since the late 1950's, Hg

  12. Electrocardiogram derived respiratory rate from QRS slopes and R-wave angle.

    PubMed

    Lázaro, Jesús; Alcaine, Alejandro; Romero, Daniel; Gil, Eduardo; Laguna, Pablo; Pueyo, Esther; Bailón, Raquel

    2014-10-01

    A method for estimating respiratory rate from electrocardiogram (ECG) signals is presented. It is based on QRS slopes and R-wave angle, which reflect respiration-induced beat morphology variations. The 12 standard leads, 3 leads from vectorcardiogram (VCG), and 2 additional non-standard leads derived from VCG loops were analyzed. The following series were studied as ECG derived respiration (EDR) signals: slope between the peak of Q and R waves, slope between the peak of R and S waves, and the R-wave angle. Information from several EDR signals was combined in order to increase the robustness of estimation. Evaluation is performed over two databases containing ECG and respiratory signals simultaneously recorded during two clinical tests with different characteristics: tilt test, representing abrupt cardiovascular changes, and stress test representing a highly non-stationary and noisy environment. A combination of QRS slopes and R-wave angle series derived from VCG leads obtained a respiratory rate estimation relative error of 0.50 ± 4.11% (measuring 99.84% of the time) for tilt test and 0.52 ± 8.99% (measuring 96.09% of the time) for stress test. These results outperform those obtained by other reported methods, both in tilt and stress testing.

  13. Variations in the accumulation, localization and rate of metabolization of selenium in mature Zea mays plants supplied with selenite or selenate.

    PubMed

    Longchamp, Mélanie; Castrec-Rouelle, Maryse; Biron, Philippe; Bariac, Thierry

    2015-09-01

    Quantification of selenium bioavailability from foods is a key challenge following the discovery of the antioxidant role of this micronutrient in human health. This study presents the uptake, accumulation and rate of metabolization in mature Zea mays plants grown in hydroponic solution supplemented with selenate or selenite. Selenium content was lower in plants supplemented with selenate and accumulated mainly in the leaves compared with selenite-treated plants where the selenium was retained in the roots. Selenite-treated grains accumulated more selenium. Selenate was metabolized less than selenite in whole plants, but in grains selenium was present exclusively as organic selenium compounds. For humans, the bioavailability of organic selenium was evaluated at 90% compared with only 50% for inorganic forms. Our results show that the potential for selenium bioavailability is increased with selenite treatment. PMID:25842318

  14. Variations in the accumulation, localization and rate of metabolization of selenium in mature Zea mays plants supplied with selenite or selenate.

    PubMed

    Longchamp, Mélanie; Castrec-Rouelle, Maryse; Biron, Philippe; Bariac, Thierry

    2015-09-01

    Quantification of selenium bioavailability from foods is a key challenge following the discovery of the antioxidant role of this micronutrient in human health. This study presents the uptake, accumulation and rate of metabolization in mature Zea mays plants grown in hydroponic solution supplemented with selenate or selenite. Selenium content was lower in plants supplemented with selenate and accumulated mainly in the leaves compared with selenite-treated plants where the selenium was retained in the roots. Selenite-treated grains accumulated more selenium. Selenate was metabolized less than selenite in whole plants, but in grains selenium was present exclusively as organic selenium compounds. For humans, the bioavailability of organic selenium was evaluated at 90% compared with only 50% for inorganic forms. Our results show that the potential for selenium bioavailability is increased with selenite treatment.

  15. Reevaluation of the O(+)(2P) reaction rate coefficients derived from Atmosphere Explorer C observations

    NASA Technical Reports Server (NTRS)

    Chang, T.; Torr, D. G.; Richards, P. G.; Solomon, S. C.

    1993-01-01

    O(+)(2P) is an important species for studies of the ionosphere and thermosphere: its emission at 7320 A can be used as a diagnostic of the thermospheric atomic oxygen density. Unfortunately, there are no laboratory measurements of the O and N2 reaction rates which are needed to determine the major sinks of (O+)(2p). We have recalculated the O and N2 reaction rates for O(+) (2P) using recent improvements in the solar EUV flux, cross sections, and photoelectron fluxes. For the standard solar EUV flux, the new N2 reaction rate of 3.4 +/- 1.5 x 10 exp -10 cu cm/s is close to the value obtained by Rusch et al. (1977), but the new O reaction rate of 4.0 +/- 1.9 x 10 exp -10 cu cm/sec is about 8 times larger. These new reaction rates are derived using neutral densities, electron density, and solar EUV fluxes measured by Atmosphere Explorer C in 1974 during solar minimum. The new theoretical emission rates are in good agreement with the data for the two orbits studied by Rusch et al.

  16. Three hen strains fed photoisomerized trans,trans CLA-rich soy oil exhibit different yolk accumulation rates and source-specific isomer deposition.

    PubMed

    Shinn, Sara E; Gilley, Alex D; Proctor, Andrew; Anthony, Nicholas B

    2015-04-01

    Most CLA chicken feeding trials used cis,trans (c,t) and trans,cis (t,c) CLA isomers to produce CLA-rich eggs, while reports of trans,trans (t,t) CLA enrichment in egg yolks are limited. The CLA yolk fatty acid profile changes and the 10-12 days of feeding needed for maximum CLA are well documented, but there is no information describing CLA accumulation during initial feed administration. In addition, no information on CLA accumulation rates in different hen strains is available. The aim of this study was to determine a mathematical model that described yolk CLA accumulation and depletion in three hen strains by using t,t CLA-rich soybean oil produced by photoisomerization. Diets of 30-week Leghorns, broilers, and jungle fowl were supplemented with 15% CLA-rich soy oil for 16 days, and eggs were collected for 32 days. Yolk fatty acid profiles were measured by GC-FID. CLA accumulation and depletion was modeled by both quadratic and piecewise regression analysis. A strong quadratic model was proposed, but it was not as effective as piecewise regression in describing CLA accumulation and depletion. Broiler hen eggs contained the greatest concentration of CLA at 3.2 mol/100 g egg yolk, then jungle fowl at 2.9 mol CLA, and Leghorns at 2.3 mol CLA. The t,t CLA isomer levels remained at 55% of total yolk CLA during CLA feeding. However, t-10,c-12 (t,c) CLA concentration increased slightly during CLA accumulation and was significantly greater than c-9,t-11 CLA. Jungle fowl had the smallest increase in yolk saturated fat with CLA yolk accumulation.

  17. Rain Fallspeeds and Rates Derived from Airborne Nadir-Pointing Doppler Radar Measurements

    NASA Technical Reports Server (NTRS)

    Heymsfield, Gerald M.; Tian, L.; Geerts, Bart

    1999-01-01

    The use of vertical-incidence Doppler velocity in addition to radar reflectivity may yield information on drop size distribution and therefore result in better rainrate estimates. Doppler velocity can provide useful information on the raindrop size distribution. Doppler velocities from a zenith-pointing radar represent the sum of the mean reflectivity-weighted hydrometeor fallspeed and the vertical air motion. Dual-parameter rain estimation methods using the Doppler velocity, require that the latter can be removed, or is negligible. Atlas et al. (1972) derived relations between Doppler velocity, reflectivity, and rain rate assuming an exponential size distribution for rain. Ulbrich (1994) expanded on this work by deriving the relation between the Doppler velocity and the reflectivity assuming a Gamma size distribution. This distribution provides a more realistic representation of the small rain drops. To get accurate information on raindrop size distributions with the above method, the air motions must be removed from the observed Doppler velocities

  18. OSL-based lateral progradation and aeolian sediment accumulation rates for the Apalachicola Barrier Island Complex, North Gulf of Mexico, Florida

    NASA Astrophysics Data System (ADS)

    Rink, W. J.; López, G. I.

    2010-11-01

    Vertical sediment cores in five separate beach ridge complexes along the north-east Gulf of Mexico Coast were recovered and dated using optically stimulated luminescence (OSL) dating of quartz: these are located on Cape San Blas (CSB), Little St. George Island (LSGI), Richardson's Hammock (RH), St. Joseph Peninsula (SJP) and Saint Vincent Island (SVI). All of these landforms are coastal barrier systems situated along a 100 km stretch of the Florida Panhandle, U.S.A. Two samples were collected for dating from each core. Ridge accumulation rates (RAR) associated with lateral progradation were calculated from the dated samples. We also determined average sediment accumulation rates (ASAR) for two intervals within each sediment core. All OSL ages within the sediment cores were found to be in stratigraphic order or in a few cases statistically indistinguishable. Moreover, all dated ridges were found to be in correct temporal sequence based on their geomorphic positions. Rapidly accreted sequences were found to be backed by St. Joseph Bay in the western region of the study area. More slowly accreted sequences were associated with the more eastern stretches of the study area backed by St. Vincent Sound and Apalachicola Bay. Our ASAR results are in accord with an Australian study of modern dune accumulation. Perhaps our most important finding is that in the barrier island environments of this north-eastern Gulf Coast region, aeolian sedimentation continues well after full vegetative cover develops and stranding of landward ridges takes place. This confirms our similar earlier observation on SVI (López and Rink, 2008). We find that up to approximately one order of magnitude lower sedimentation rates occur after an initial period of more rapid aeolian accumulation for the vertical intervals studied in foredune ridges. Lateral progradation rates of ridge sequences were highly variable within the study area, ranging from 92 to 848 m/100 years, but we did find agreement

  19. Kartogenin, transforming growth factor-β1 and bone morphogenetic protein-7 coordinately enhance lubricin accumulation in bone-derived mesenchymal stem cells.

    PubMed

    Liu, Chun; Ma, Xueqin; Li, Tao; Zhang, Qiqing

    2015-09-01

    Osteoarthritis, a common joint degeneration, can cause breakdown of articular cartilage with the presence of lubricin metabolic abnormalities. Lubricin is a multi-level chondroprotective mucinous glycoprotein in articular joints. Joint defect and infection is elevated and accompanied by accelerated cartilage lesions involving degradation and loss of lubricin. However, a novel, heterocyclic compound called kartogenin (KGN) was discovered to stimulate chondrogenic differentiation of bone-derived mesenchymal stem cells (BMSCs). And the synergistic effect of transforming growth factor-β1 (TGF-β1) and bone morphogenetic protein-7 (BMP-7) could provoke lubricin accumulation. This paper attempted to explore the connection between accumulation of lubricin and the effect of TGF-β1, BMP-7 and/or KGN. Hence, we investigated the expression and secretion of lubricin in BMSCs treated with different combinations of TGF-β1, BMP-7, and/or KGN. Using an in vitro BMSCs system, we observed the content of lubricin from BMSCs treated with TGF-β1, BMP-7, and KGN was the highest at both the protein level and the gene level. The accumulation of lubricin was enhanced coordinately by the increase of synthesis and decrease of degradation possibly via c-Myc and adamts5 pathway. These results further suggested that supplementation of the defect parts with lubricin by using growth factors and small molecules showed a promising potential on preventing joint deterioration in patients with acquired or genetic deficiency of lubricin in the future of regenerative medicine.

  20. Inhibition of dendritic cell differentiation and accumulation of myeloid-derived suppressor cells in cancer is regulated by S100A9 protein

    PubMed Central

    Cheng, Pingyan; Corzo, Cesar A.; Luetteke, Noreen; Yu, Bin; Nagaraj, Srinivas; Bui, Marylin M.; Ortiz, Myrna; Nacken, Wolfgang; Sorg, Clemens; Vogl, Thomas; Roth, Johannes; Gabrilovich, Dmitry I.

    2008-01-01

    Accumulation of myeloid-derived suppressor cells (MDSCs) associated with inhibition of dendritic cell (DC) differentiation is one of the major immunological abnormalities in cancer and leads to suppression of antitumor immune responses. The molecular mechanism of this phenomenon remains unclear. We report here that STAT3-inducible up-regulation of the myeloid-related protein S100A9 enhances MDSC production in cancer. Mice lacking this protein mounted potent antitumor immune responses and rejected implanted tumors. This effect was reversed by administration of wild-type MDSCs from tumor-bearing mice to S100A9-null mice. Overexpression of S100A9 in cultured embryonic stem cells or transgenic mice inhibited the differentiation of DCs and macrophages and induced accumulation of MDSCs. This study demonstrates that tumor-induced up-regulation of S100A9 protein is critically important for accumulation of MDSCs and reveals a novel molecular mechanism of immunological abnormalities in cancer. PMID:18809714

  1. Tectonic control on 10Be-derived erosion rates in the Garhwal Himalaya, India

    NASA Astrophysics Data System (ADS)

    Scherler, Dirk; Bookhagen, Bodo; Strecker, Manfred R.

    2014-02-01

    Erosion in the Himalaya is responsible for one of the greatest mass redistributions on Earth and has fueled models of feedback loops between climate and tectonics. Although the general trends of erosion across the Himalaya are reasonably well known, the relative importance of factors controlling erosion is less well constrained. Here we present 25 10Be-derived catchment-averaged erosion rates from the Yamuna catchment in the Garhwal Himalaya, northern India. Tributary erosion rates range between ~0.1 and 0.5 mm yr-1 in the Lesser Himalaya and ~1 and 2 mm yr-1 in the High Himalaya, despite uniform hillslope angles. The erosion-rate data correlate with catchment-averaged values of 5 km radius relief, channel steepness indices, and specific stream power but to varying degrees of nonlinearity. Similar nonlinear relationships and coefficients of determination suggest that topographic steepness is the major control on the spatial variability of erosion and that twofold to threefold differences in annual runoff are of minor importance in this area. Instead, the spatial distribution of erosion in the study area is consistent with a tectonic model in which the rock uplift pattern is largely controlled by the shortening rate and the geometry of the Main Himalayan Thrust fault (MHT). Our data support a shallow dip of the MHT underneath the Lesser Himalaya, followed by a midcrustal ramp underneath the High Himalaya, as indicated by geophysical data. Finally, analysis of sample results from larger main stem rivers indicates significant variability of 10Be-derived erosion rates, possibly related to nonproportional sediment supply from different tributaries and incomplete mixing in main stem channels.

  2. 10Be-derived Himalayan denudation rates and sediment budgets in the Ganga basin

    NASA Astrophysics Data System (ADS)

    Lupker, Maarten; Blard, Pierre-Henri; Lavé, Jérôme; France-Lanord, Christian; Leanni, Laetitia; Puchol, Nicolas; Charreau, Julien; Bourlès, Didier

    2012-06-01

    The Himalayas represent the archetype of mountain building due to active continental collision and are considered in many studies as the locus of intense interactions between climate, denudation and tectonics. Estimates of modern denudation rates across the entire range remain, however, relatively sparse. In this study, in situ-produced cosmogenic 10Be concentrations were measured in detritic quartz in order to determine basin-scale denudation rates for the central part of the Himalayan range. River sand was sampled over several years in the main trans-Himalayan rivers, from the Himalayan front to the Ganga outlet in Bangladesh. The calculated 10Be denudation rates of the trans-Himalayan river basins range from 0.5 to 2.4 mm yr-1 (average 1.3 mm yr-1) and vary by up to a factor of 3 between sampling years. These denudation rates strongly contrast with the 0.007 mm yr-1 denudation rate of southern tributary basins draining the Indian craton. This work also shows that in the Ganga basin, no systematic evolution of average 10Be concentrations is observed during floodplain transfer, implying that distal samples can be used to estimate the integrated denudation rate of the whole central Himalayan range. Samples from the Ganga in Bangladesh display remarkably low variability in 10Be concentration, implying an average Himalayan denudation rate of 1.0-1.1 mm yr-1. However, within the floodplain, several samples suggest a recent perturbation of sediment transport dynamics with a recent increase in the relative sediment contribution from southern tributaries. The Himalayan sediment flux, deduced from the 10Be denudation rate of the range, is 610±230 Mt yr-1. This flux is consistent, within uncertainty, with sediment fluxes derived from sediment gauging. The similarity of the two flux estimates suggests that Himalayan erosion fluxes have remained stable over the last centuries, even if the large uncertainties associated with each method hamper more precise assessments.

  3. Germline mutation rates and the long-term phenotypic effects of mutation accumulation in wild-type laboratory mice and mutator mice.

    PubMed

    Uchimura, Arikuni; Higuchi, Mayumi; Minakuchi, Yohei; Ohno, Mizuki; Toyoda, Atsushi; Fujiyama, Asao; Miura, Ikuo; Wakana, Shigeharu; Nishino, Jo; Yagi, Takeshi

    2015-08-01

    The germline mutation rate is an important parameter that affects the amount of genetic variation and the rate of evolution. However, neither the rate of germline mutations in laboratory mice nor the biological significance of the mutation rate in mammalian populations is clear. Here we studied genome-wide mutation rates and the long-term effects of mutation accumulation on phenotype in more than 20 generations of wild-type C57BL/6 mice and mutator mice, which have high DNA replication error rates. We estimated the base-substitution mutation rate to be 5.4 × 10(-9) (95% confidence interval = 4.6 × 10(-9)-6.5 × 10(-9)) per nucleotide per generation in C57BL/6 laboratory mice, about half the rate reported in humans. The mutation rate in mutator mice was 17 times that in wild-type mice. Abnormal phenotypes were 4.1-fold more frequent in the mutator lines than in the wild-type lines. After several generations, the mutator mice reproduced at substantially lower rates than the controls, exhibiting low pregnancy rates, lower survival rates, and smaller litter sizes, and many of the breeding lines died out. These results provide fundamental information about mouse genetics and reveal the impact of germline mutation rates on phenotypes in a mammalian population.

  4. Germline mutation rates and the long-term phenotypic effects of mutation accumulation in wild-type laboratory mice and mutator mice

    PubMed Central

    Uchimura, Arikuni; Higuchi, Mayumi; Minakuchi, Yohei; Ohno, Mizuki; Toyoda, Atsushi; Fujiyama, Asao; Miura, Ikuo; Wakana, Shigeharu; Nishino, Jo; Yagi, Takeshi

    2015-01-01

    The germline mutation rate is an important parameter that affects the amount of genetic variation and the rate of evolution. However, neither the rate of germline mutations in laboratory mice nor the biological significance of the mutation rate in mammalian populations is clear. Here we studied genome-wide mutation rates and the long-term effects of mutation accumulation on phenotype in more than 20 generations of wild-type C57BL/6 mice and mutator mice, which have high DNA replication error rates. We estimated the base-substitution mutation rate to be 5.4 × 10−9 (95% confidence interval = 4.6 × 10−9–6.5 × 10−9) per nucleotide per generation in C57BL/6 laboratory mice, about half the rate reported in humans. The mutation rate in mutator mice was 17 times that in wild-type mice. Abnormal phenotypes were 4.1-fold more frequent in the mutator lines than in the wild-type lines. After several generations, the mutator mice reproduced at substantially lower rates than the controls, exhibiting low pregnancy rates, lower survival rates, and smaller litter sizes, and many of the breeding lines died out. These results provide fundamental information about mouse genetics and reveal the impact of germline mutation rates on phenotypes in a mammalian population. PMID:26129709

  5. Patient-derived xenograft (PDX) tumors increase growth rate with time

    PubMed Central

    Pearson, Alexander T.; Finkel, Kelsey A.; Warner, Kristy A.; Nör, Felipe; Tice, David; Martins, Manoela D.; Jackson, Trachette L.; Nör, Jacques E.

    2016-01-01

    Patient-derived xenograft (PDX) models are frequently used for translational cancer research, and are assumed to behave consistently as the tumor ages. However, growth rate constancy as a function of time is unclear. Notably, variable PDX growth rates over time might have implications for the interpretation of translational studies. We characterized four PDX models through several in vivo passages from primary human head and neck squamous cell carcinoma and salivary gland adenoid cystic carcinoma. We developed a mathematical approach to merge growth data from different passages into a single measure of relative tumor volume normalized to study initiation size. We analyzed log-relative tumor volume increase with linear mixed effect models. Two oral pathologists analyzed the PDX tissues to determine if histopathological feature changes occurred over in vivo passages. Tumor growth rate increased over time. This was determined by repeated measures linear regression statistical analysis in four different PDX models. A quadratic statistical model for the temporal effect predicted the log-relative tumor volume significantly better than a linear time effect model. We found a significant correlation between passage number and histopathological features of higher tumor grade. Our mathematical treatment of PDX data allows statistical analysis of tumor growth data over long periods of time, including over multiple passages. Non-linear tumor growth in our regression models revealed the exponential growth rate increased over time. The dynamic tumor growth rates correlated with quantifiable histopathological changes that related to passage number in multiple types of cancer. PMID:26783960

  6. Patient-derived xenograft (PDX) tumors increase growth rate with time.

    PubMed

    Pearson, Alexander T; Finkel, Kelsey A; Warner, Kristy A; Nör, Felipe; Tice, David; Martins, Manoela D; Jackson, Trachette L; Nör, Jacques E

    2016-02-16

    Patient-derived xenograft (PDX) models are frequently used for translational cancer research, and are assumed to behave consistently as the tumor ages. However, growth rate constancy as a function of time is unclear. Notably, variable PDX growth rates over time might have implications for the interpretation of translational studies. We characterized four PDX models through several in vivo passages from primary human head and neck squamous cell carcinoma and salivary gland adenoid cystic carcinoma. We developed a mathematical approach to merge growth data from different passages into a single measure of relative tumor volume normalized to study initiation size. We analyzed log-relative tumor volume increase with linear mixed effect models. Two oral pathologists analyzed the PDX tissues to determine if histopathological feature changes occurred over in vivo passages. Tumor growth rate increased over time. This was determined by repeated measures linear regression statistical analysis in four different PDX models. A quadratic statistical model for the temporal effect predicted the log-relative tumor volume significantly better than a linear time effect model. We found a significant correlation between passage number and histopathological features of higher tumor grade. Our mathematical treatment of PDX data allows statistical analysis of tumor growth data over long periods of time, including over multiple passages. Non-linear tumor growth in our regression models revealed the exponential growth rate increased over time. The dynamic tumor growth rates correlated with quantifiable histopathological changes that related to passage number in multiple types of cancer.

  7. A new method for deriving steady-state rate equations suitable for manual or computer use.

    PubMed Central

    Indge, K J; Childs, R E

    1976-01-01

    A schematic method for the derivation of steady-state enzyme rate equations by using the Wang algebra is described. The method is simple, easy to learn and offers a substantial decrease in analytical effort over previously published algorithms. Being essentially an algebraic procedure the method can be readily computerized. Computer programs in BASIC and ALGOL languages have been deposited as Supplementary Publication SUP 50065 (19 pages) at the British Library (Lending Division), Boston Spa, Wetherby, W. Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1976). 153, 5. PMID:949319

  8. Derivative expansion and gauge independence of the false vacuum decay rate in various gauges

    NASA Astrophysics Data System (ADS)

    Metaxas, D.

    2001-04-01

    In theories with radiative symmetry breaking, the calculation of the false vacuum decay rate requires the inclusion of higher-order terms in the derivative expansion of the effective action. I show here that, in the case of covariant gauges, the presence of infrared singularities forbids the consistent calculation by keeping the lowest-order terms. The situation is remedied, however, in the case of Rξ gauges. Using the Nielsen identities I show that the final result is gauge independent for generic values of the gauge parameter v that are not anomalously small.

  9. Malfunction in Mitochondrial β-Oxidation Contributes to Lipid Accumulation in Hepatocyte-Like Cells Derived from Citrin Deficiency-Induced Pluripotent Stem Cells.

    PubMed

    Kim, Yeji; Choi, Jung-Yun; Lee, Sang-Hee; Lee, Beom-Hee; Yoo, Han-Wook; Han, Yong-Mahn

    2016-04-15

    Citrin deficiency (CD) is a recessive genetic disorder caused by mutations in the citrin gene SLC25A13. CD causes various symptoms related to nutrient metabolism such as urea cycle failure, abnormal amino acid levels, and fatty liver. To understand the pathophysiology of CD, the molecular phenotypes were investigated using induced pluripotent stem cells derived from fibroblasts of CD patient (CD-iPSCs). In this study, we demonstrate that aberrant mitochondrial β-oxidation may lead to fatty liver in CD patients. CD-iPSCs normally differentiated into hepatocytes, similar to wild-type iPSCs (WT-iPSCs). However, hepatocytes derived from CD-iPSCs (CD-HLCs) did not exhibit ureogenesis. Cellular triglyceride and lipid granule levels were significantly increased in CD-HLCs compared with WT-HLCs. Peroxisome proliferator-activated receptor-α (PPAR-α) and its target genes which are involved in mitochondrial β-oxidation were downregulated in CD-HLCs, and treatment with a PPAR-α agonist partially reduced the lipid accumulation in CD-HLCs. In addition, the mitochondria in CD-HLCs exhibited abnormal morphologies. Based on these observations, we conclude that the lipid accumulation in CD-HLCs results from dysfunctional mitochondrial β-oxidation and abnormal mitochondrial structure.

  10. HD iPSC-derived neural progenitors accumulate in culture and are susceptible to BDNF withdrawal due to glutamate toxicity.

    PubMed

    Mattis, Virginia B; Tom, Colton; Akimov, Sergey; Saeedian, Jasmine; Østergaard, Michael E; Southwell, Amber L; Doty, Crystal N; Ornelas, Loren; Sahabian, Anais; Lenaeus, Lindsay; Mandefro, Berhan; Sareen, Dhruv; Arjomand, Jamshid; Hayden, Michael R; Ross, Christopher A; Svendsen, Clive N

    2015-06-01

    Huntington's disease (HD) is a fatal neurodegenerative disease, caused by expansion of polyglutamine repeats in the Huntingtin gene, with longer expansions leading to earlier ages of onset. The HD iPSC Consortium has recently reported a new in vitro model of HD based on the generation of induced pluripotent stem cells (iPSCs) from HD patients and controls. The current study has furthered the disease in a dish model of HD by generating new non-integrating HD and control iPSC lines. Both HD and control iPSC lines can be efficiently differentiated into neurons/glia; however, the HD-derived cells maintained a significantly greater number of nestin-expressing neural progenitor cells compared with control cells. This cell population showed enhanced vulnerability to brain-derived neurotrophic factor (BDNF) withdrawal in the juvenile-onset HD (JHD) lines, which appeared to be CAG repeat-dependent and mediated by the loss of signaling from the TrkB receptor. It was postulated that this increased death following BDNF withdrawal may be due to glutamate toxicity, as the N-methyl-d-aspartate (NMDA) receptor subunit NR2B was up-regulated in the cultures. Indeed, blocking glutamate signaling, not just through the NMDA but also mGlu and AMPA/Kainate receptors, completely reversed the cell death phenotype. This study suggests that the pathogenesis of JHD may involve in part a population of 'persistent' neural progenitors that are selectively vulnerable to BDNF withdrawal. Similar results were seen in adult hippocampal-derived neural progenitors isolated from the BACHD model mouse. Together, these results provide important insight into HD mechanisms at early developmental time points, which may suggest novel approaches to HD therapeutics. PMID:25740845

  11. Sediment accumulation rates and high-resolution stratigraphy of recent fluvial suspension deposits in various fluvial settings, Morava River catchment area, Czech Republic

    NASA Astrophysics Data System (ADS)

    Sedláček, Jan; Bábek, Ondřej; Kielar, Ondřej

    2016-02-01

    We present a comprehensive study concerning sedimentary processes in fluvial sediment traps within the Morava River catchment area (Czech Republic) involving three dammed reservoirs, four meanders and oxbow lakes, and several natural floodplain sites. The objective of the study was to determine sediment accumulation rates (SAR), estimate erosion rates, calculating these using a combination of the 137Cs method and historical data. Another purpose of this study was to provide insight into changing erosion and accumulation rates over the last century. Extensive water course modifications were carried out in the Morava River catchment area during the twentieth century, which likely affected sedimentation rates along the river course. Other multiproxy stratigraphic methods (X-ray densitometry, magnetic susceptibility, and visible-light reflectance spectrometry) were applied to obtain additional information about sediment infill. Sediment stratigraphy revealed distinct distal-to-proximal patterns, especially in reservoirs. Granulometrically, silts and sandy silts prevailed in sediments. Oxbow lakes and meanders contained larger amounts of clay and organic matter, which is the main difference between them and reservoirs. Pronounced 137Cs peaks were recorded in all studied cores (maximum 377 Bq·kg- 1), thus indicating Chernobyl fallout from 1986 or older events. Calculated sediment accumulation rates were lowest in distal parts of reservoirs (0.13-0.58 cm/y) and floodplains (0.45-0.88 cm/y), moderately high rates were found in proximal parts of reservoirs and oxbow lakes (2.27-4.4 cm/y), and the highest rates in some oxbow lakes located near the river (6-8 cm/y). The frequency of the inundation still can be high in some natural areas as in the Litovelské Pomoraví protected area, whereas the decreasing frequency of the inundation in other modified parts can contribute to a lower sedimentation rate. The local effects such as difference between SARs in oxbow lakes and

  12. Solvent effect on intramolecular electron transfer rates of mixed-valence biferrocene monocation derivatives.

    PubMed

    Masuda, Y; Shimizu, C

    2006-06-01

    Intramolecular electron transfer (ET) rates in various solvents of mixed-valence biferrocene monocation (Fe(II), Fe(III)) and the 1',1' ''-diiodo and 1',1' ''-diethyl derivatives (respectively abbreviated as BFC(+), I(2)BFC(+), and Et(2)BFC(+)) were determined by means of the spin-lattice relaxation times of the protons, taking into account the local magnetic field fluctuation caused by the electron hopping between the two ferrocene units. We also determined the ET rates of a mixed-valence diferrocenylacetylene monocation (DFA(+)) in order to examine the effect of the insertion of an acetylene bridge between the two ferrocene units. The insertion of the bridge decreased the ET rate, while the effect of substitution on the cyclopentadienyl rings on the rate was minor. The observed rates for each mixed-valence monocation in various solvents did not correlate with the reorganization energies, but we did find a significant contribution of the solvent dynamics. The observed rates were considerably higher than those expected on the basis of the Sumi-Marcus-Nalder model in which the solvents were regarded as dielectric continua. The slope of the logarithm plot of the pre-exponential factors in various solvents for each mixed-valence monocation versus the inverse of the longitudinal dielectric relaxation times of the solvents was significantly smaller than unity, and the slope for DFA(+) was larger than those for BFC(+), I(2)BFC(+), and Et(2)BFC(+). These results were ascribed to a partial contribution of the dielectric friction to the dynamics along the solvent coordinate; the extent of the contribution decreased with a reduction in the ET distance. For the dynamics along the solvent coordinate of the ET reactions in methanol, the observed rates indicated an important contribution by the minor dielectric relaxation components with faster relaxation times, rather than the major component with an extraordinarily long relaxation time.

  13. Rain rate duration statistics derived from the Mid-Atlantic coast rain gauge network

    NASA Technical Reports Server (NTRS)

    Goldhirsh, Julius

    1993-01-01

    A rain gauge network comprised of 10 tipping bucket rain gauges located in the Mid-Atlantic coast of the United States has been in continuous operation since June 1, 1986. Rain rate distributions and estimated slant path fade distributions at 20 GHz and 30 GHz covering the first five year period were derived from the gauge network measurements, and these results were described by Goldhirsh. In this effort, rain rate time duration statistics are presented. The rain duration statistics are of interest for better understanding the physical nature of precipitation and to present a data base which may be used by modelers to convert to slant path fade duration statistics. Such statistics are important for better assessing optimal coding procedures over defined bandwidths.

  14. Increasing sediment accumulation rates in La Fonera (Palamós) submarine canyon axis and their relationship with bottom trawling activities

    NASA Astrophysics Data System (ADS)

    Puig, P.; Martín, J.; Masqué, P.; Palanques, A.

    2015-10-01

    Previous studies conducted in La Fonera (Palamós) submarine canyon (NW Mediterranean) found that trawling activities along the canyon flanks cause resuspension and transport of sediments toward the canyon axis. 210Pb chronology supported by 137Cs dating applied to a sediment core collected at 1750 m in 2002 suggested a doubling of the sediment accumulation rate since the 1970s, coincident with the rapid industrialization of the local trawling fleet. The same canyon area has been revisited a decade later, and new data are consistent with a sedimentary regime shift during the 1970s and also suggest that the accumulation rate during the last decade could be greater than expected, approaching ~2.4 cm yr-1 (compared to ~0.25 cm yr-1 pre-1970s). These results support the hypothesis that commercial bottom trawling can substantially affect sediment dynamics and budgets on continental margins, eventually initiating the formation of anthropogenic depocenters in submarine canyon environments.

  15. Derivation of heating rate dependent exposure strategies for the selective laser melting of thermoplastic polymers

    NASA Astrophysics Data System (ADS)

    Drummer, Dietmar; Drexler, Maximilian; Wudy, Katrin

    2015-05-01

    The selective laser melting of polymer powder is for rapid prototyping applications an established technology, although a lack in basic process knowledge appears. Considering demands of series production the selective laser melting technique is faced with varies challenges concerning processable material systems, process strategies and part properties. Consequently basic research is necessary to shift from rapid prototyping to rapid manufacturing of small lot sized series. Based on basic research the high potential of selective laser melting for the production of complex parts without any tools can be opened up. For the derivation of part quality increasing process strategies knowledge about interactions between sub-processes of selective laser melting and resulting part properties is necessary. The selective laser melting consists of three major sub-processes: Geometry exposure, tempering and powder feeding. According to the interaction of sub-processes resulting temperature fields during the selective laser melting process determine the part properties by changing micro structural pore number and distribution. Beneath absolute temperatures also the time-dependency of the thermal fields influences the porosity of molten parts. Present process strategies tend to decrease building time by increasing scanning speed and laser power. Although the absolute energy input into the material is constant for increasing scanning speed and laser power in the same ratio, time dependent material effects are neglected. The heating rate is a combined parameter derived from absolute temperature and time. Within the paper the authors analyze the basic interactions between different heating rates and part properties (e.g. porosity, mechanical strengths). Therefore with different heating rates produced specimens are analyzed with imaging technologies as well as mechanical tests. Based on the done basic investigations new heating rate dependent process strategies can be established

  16. Accuracy of heart strain rate calculation derived from Doppler tissue velocity data

    NASA Astrophysics Data System (ADS)

    Santos, Andres; Ledesma-Carbayo, Maria J.; Malpica, Norberto; Desco, Manuel; Antoranz, Jose C.; Marcos-Alberca, Pedro; Garcia-Fernandez, Miguel A.

    2001-05-01

    Strain Rate (SR) Imaging is a recent imaging technique that provides information about regional myocardial deformation by measuring local compression and expansion rates. SR can be obtained by calculating the local in-plane velocity gradients along the ultrasound beam from Doppler Tissue velocity data. However, SR calculations are very dependent on the image noise and artifacts, and different calculation algorithms may provide inconsistent results. This paper compares techniques to calculate SR. 2D Doppler Tissue Images (DTI) are acquired with an Acuson Sequoia scanner. Noise was measured with the aid of a rotating phantom. Processing is performed on polar coordinates. For each image, after removal of black spot artifacts by a selective median filter, two different SR calculation methods have been implemented. In the first one, SR is computed as the discrete velocity derivative, and noise is reduced with a variable-width gaussian filter. In the second method a smoothing cubic spine is calculated for every scan line according to the noise level and the derivative is obtained from an analytical expression. Both methods have been tested with DTI data from synthetic phantoms and normal volunteers. Results show that noise characteristics, border effects and the adequate scale are critical to obtain meaningful results.

  17. How Integrated Management Strategies Promote Protein Quality of Cotton Embryos: High Levels of Soil Available N, N Assimilation and Protein Accumulation Rate

    PubMed Central

    Yang, HongKun; Meng, YaLi; Chen, BingLin; Zhang, XingYue; Wang, YouHua; Zhao, WenQing; Zhou, ZhiGuo

    2016-01-01

    Cottonseed is widely used as a source of ruminant feed and for industrial purposes. Therefore, there is a tremendous need to improve the nutritional value of cotton embryos. In this study, a conventional management (CM) and two integrated cotton management strategies (IMS1, IMS2) were performed at two soil fertility levels to study the relationships among soil N, N assimilation, embryonic protein accumulation and protein quality. The levels of proteins, essential amino acids, and semi-essential amino acids, especially those of glutamate, lysine, and methionine, were higher in IMS1 and IMS2 embryos than in CM embryos. These changes were significantly positively correlated with the soil-available N content, glutamine synthetase activity and peak value of protein accumulation rate and were negatively correlated with the free amino acid level. These results illustrated that integrated management strategies, especially the rates and timing of N application, raise the level of soil available N, which is beneficial for N assimilation in developing cotton embryos. The protein content was limited by the rate of protein accumulation rather than by the free amino acid content. The combination of target yield fertilization, a growth-driven N application schedule, a high plant density and the seedling raising with bio-organic fertilizer can substantially improve protein quality in cotton embryos, especially at a soil with low soil organic matter and total nitrogen. PMID:27532007

  18. How Integrated Management Strategies Promote Protein Quality of Cotton Embryos: High Levels of Soil Available N, N Assimilation and Protein Accumulation Rate.

    PubMed

    Yang, HongKun; Meng, YaLi; Chen, BingLin; Zhang, XingYue; Wang, YouHua; Zhao, WenQing; Zhou, ZhiGuo

    2016-01-01

    Cottonseed is widely used as a source of ruminant feed and for industrial purposes. Therefore, there is a tremendous need to improve the nutritional value of cotton embryos. In this study, a conventional management (CM) and two integrated cotton management strategies (IMS1, IMS2) were performed at two soil fertility levels to study the relationships among soil N, N assimilation, embryonic protein accumulation and protein quality. The levels of proteins, essential amino acids, and semi-essential amino acids, especially those of glutamate, lysine, and methionine, were higher in IMS1 and IMS2 embryos than in CM embryos. These changes were significantly positively correlated with the soil-available N content, glutamine synthetase activity and peak value of protein accumulation rate and were negatively correlated with the free amino acid level. These results illustrated that integrated management strategies, especially the rates and timing of N application, raise the level of soil available N, which is beneficial for N assimilation in developing cotton embryos. The protein content was limited by the rate of protein accumulation rather than by the free amino acid content. The combination of target yield fertilization, a growth-driven N application schedule, a high plant density and the seedling raising with bio-organic fertilizer can substantially improve protein quality in cotton embryos, especially at a soil with low soil organic matter and total nitrogen. PMID:27532007

  19. How Integrated Management Strategies Promote Protein Quality of Cotton Embryos: High Levels of Soil Available N, N Assimilation and Protein Accumulation Rate.

    PubMed

    Yang, HongKun; Meng, YaLi; Chen, BingLin; Zhang, XingYue; Wang, YouHua; Zhao, WenQing; Zhou, ZhiGuo

    2016-01-01

    Cottonseed is widely used as a source of ruminant feed and for industrial purposes. Therefore, there is a tremendous need to improve the nutritional value of cotton embryos. In this study, a conventional management (CM) and two integrated cotton management strategies (IMS1, IMS2) were performed at two soil fertility levels to study the relationships among soil N, N assimilation, embryonic protein accumulation and protein quality. The levels of proteins, essential amino acids, and semi-essential amino acids, especially those of glutamate, lysine, and methionine, were higher in IMS1 and IMS2 embryos than in CM embryos. These changes were significantly positively correlated with the soil-available N content, glutamine synthetase activity and peak value of protein accumulation rate and were negatively correlated with the free amino acid level. These results illustrated that integrated management strategies, especially the rates and timing of N application, raise the level of soil available N, which is beneficial for N assimilation in developing cotton embryos. The protein content was limited by the rate of protein accumulation rather than by the free amino acid content. The combination of target yield fertilization, a growth-driven N application schedule, a high plant density and the seedling raising with bio-organic fertilizer can substantially improve protein quality in cotton embryos, especially at a soil with low soil organic matter and total nitrogen.

  20. Mantle-derived helium in sedimentary basins of Central Mediterranean: Geologic and tectonic constrains on fluids accumulation and migration.

    NASA Astrophysics Data System (ADS)

    Caracausi, Antonio; Grassa, Fausto; Pennino, Valentina; Rizzo, Andrea; Sulli, Attilio

    2013-04-01

    The geodynamics of the central Mediterranean is characterized by the interaction between the European plate and the African one. In this setting Sicily is a sector of the Appenine-Maghrebide accretionary prism, which is located between two areas affected by extensional tectonics (Sicily Channel to the south and the Thyrrenian back arc basin to the north). In the present study we present the first dataset of helium isotopic composition measured in fluids released from the central-western Sicily. With the aim to constrain the transfer system of fluids in this area we relate the results of geochemical investigations with the stratigraphy and structural setting, derived from field geology, deep boreholes and new seismic reflection, gravimetry and magnetometry data. Significant mantle-derived helium (0.4

  1. Slip-rates along the Chaman fault: Implication for transient strain accumulation and strain partitioning along the western Indian plate margin

    NASA Astrophysics Data System (ADS)

    Ul-Hadi, Shams; Khan, Shuhab D.; Owen, Lewis A.; Khan, Abdul S.; Hedrick, Kathryn A.; Caffee, Marc W.

    2013-11-01

    The Chaman fault in Western Pakistan marks the western collision boundary between the Indian and Eurasian plates and connects the Makran subduction zone to the Himalayan convergence zone. Geomorphic-scale slip-rates along an active strand of the Chaman fault are added to the sporadic data set of this poorly investigated transform system. Field investigations coupled with high-resolution GeoEye-1 satellite data of an alluvial fan surface (Bostankaul alluvial fan) show ~ 1150 m left-lateral offset by the fault since the formation of the alluvial fan surface. A weighted mean 10Be exposure age of 34.8 ± 3 kyr for the Bostankaul alluvial surface yields a slip-rate of 33.3 ± 3.0 mm/yr. This rate agrees with the geologically defined slip-rates along the Chaman fault, but is approximately twice as large as that inferred from the decade-long global positioning system measurements of 18 ± 1 mm/yr. The contrast in geomorphic and geodetic slip-rates along the Chaman fault, like other major intra-continental strike-slip faults, has two major implications: 1) the geodetic rates might represent a period of reduced displacement as compared to the averaged Late Pleistocene rate because of transient variations in rates of elastic strain accumulation; or 2) strain partitioning within the plate boundary zone. While strain partitioning could be the reason of slip-rate variations within the western Indian plate boundary zone, transient strain accumulation could explain contrasting slip-rates along the Chaman fault at this stage in its poorly understood seismic cycle.

  2. Shallow Sediment Trace Metal Concentrations and Short-Term Accumulation Rates in the Neponset River Estuary, Massachusetts, USA

    NASA Astrophysics Data System (ADS)

    Spencer, J. R.; Zhu, J.; Olsen, C. R.

    2010-12-01

    The Neponset River estuary is a small estuary that drains into the Boston Harbor on the east coast of the United States. It is also a highly urbanized estuary and has a long history of urban development over 450 years. In July 2006, six sediment cores were collected in the Neponset River estuary to examine particle dynamics and sediment accumulation via radionuclide (Beryllium-7) dating, and to determine sediment metal concentrations (As, Cu, Pb, and Zn) via ED-XRF measurements. Measured sediment Be-7 profiles indicate various sedimentation environments, where sediment accumulation, resuspension or redeposition is likely to occur. High metal concentrations were often corresponding to high Be-7 inventories in sediment cores. Possible sources of trace metal contaminants in the water column include: storm water run-off, Combined Sewer Overflows (CSOs), a well-documented industrial pollution event that occurred upstream in the early to mid twentieth century, and the resuspension of sediment. Existing and future data will provide baseline information for quantifying the effects of the proposed and pending environmental restoration project, which includes the removal of the Baker Dam. The combined pre- and post-Dam removal data may then be used in cost-benefit analyses for other similar estuarine restoration projects.

  3. Influence of satellite-derived photolysis rates and NOx emissions on Texas ozone modeling

    NASA Astrophysics Data System (ADS)

    Tang, W.; Cohan, D. S.; Pour-Biazar, A.; Lamsal, L. N.; White, A. T.; Xiao, X.; Zhou, W.; Henderson, B. H.; Lash, B. F.

    2015-02-01

    Uncertain photolysis rates and emission inventory impair the accuracy of state-level ozone (O3) regulatory modeling. Past studies have separately used satellite-observed clouds to correct the model-predicted photolysis rates, or satellite-constrained top-down NOx emissions to identify and reduce uncertainties in bottom-up NOx emissions. However, the joint application of multiple satellite-derived model inputs to improve O3 state implementation plan (SIP) modeling has rarely been explored. In this study, Geostationary Operational Environmental Satellite (GOES) observations of clouds are applied to derive the photolysis rates, replacing those used in Texas SIP modeling. This changes modeled O3 concentrations by up to 80 ppb and improves O3 simulations by reducing modeled normalized mean bias (NMB) and normalized mean error (NME) by up to 0.1. A sector-based discrete Kalman filter (DKF) inversion approach is incorporated with the Comprehensive Air Quality Model with extensions (CAMx)-decoupled direct method (DDM) model to adjust Texas NOx emissions using a high-resolution Ozone Monitoring Instrument (OMI) NO2 product. The discrepancy between OMI and CAMx NO2 vertical column densities (VCDs) is further reduced by increasing modeled NOx lifetime and adding an artificial amount of NO2 in the upper troposphere. The region-based DKF inversion suggests increasing NOx emissions by 10-50% in most regions, deteriorating the model performance in predicting ground NO2 and O3, while the sector-based DKF inversion tends to scale down area and nonroad NOx emissions by 50%, leading to a 2-5 ppb decrease in ground 8 h O3 predictions. Model performance in simulating ground NO2 and O3 are improved using sector-based inversion-constrained NOx emissions, with 0.25 and 0.04 reductions in NMBs and 0.13 and 0.04 reductions in NMEs, respectively. Using both GOES-derived photolysis rates and OMI-constrained NOx emissions together reduces modeled NMB and NME by 0.05, increases the model

  4. NOX1-induced accumulation of reactive oxygen species in abdominal fat-derived mesenchymal stromal cells impinges on long-term proliferation.

    PubMed

    Sela, M; Tirza, G; Ravid, O; Volovitz, I; Solodeev, I; Friedman, O; Zipori, D; Gur, E; Krelin, Y; Shani, N

    2015-04-16

    Mesenchymal stromal cells (MSCs) are multipotent and can be derived from different adult tissues including fat. Our repeated attempts to produce long-term proliferative cultures of rat abdominal adipose stem cells (aASCs) under normal oxygen concentration (21%) were unsuccessful. We set to examine the events controlling this cytostasis of aASCs and found that it resulted from overproduction of reactive oxygen species (ROS) that led to apoptosis. ROS overproduction in aASCs was accompanied by increased expression of NOX1 but not of NOX2 or NOX4. NOX family members are an important source of intracellular ROS pointing to NOX1 involvement in ROS accumulation. This was verified when aASCs that were grown under 3% oxygen conditions expanded long term, displaying reduced NOX1 expression and decreased ROS accumulation. NOX1 involvement in aASC cytostasis was reaffirmed when cells that were expanded under normoxic conditions in the presence of a specific NOX1 inhibitor, ML171, demonstrated reduced ROS accumulation, reduced apoptosis and long-term expansion. aASC expansion arrest was accompanied also by a weak fat differentiation and migratory potential, which was enhanced by NOX1 inhibition. This suggests an inhibitory role for NOX1-induced ROS overproduction on aASCs, their fat differentiation and migratory potential. In contrast to aASCs, similar cells produced from subcutaneous fat were easily expanded in normoxic cultures, exhibiting low ROS concentrations, a low number of apoptotic cells and improved fat differentiation and migration. Taken together, our results show, for the first time, that NOX1-induced ROS accumulation halts ASC expansion and reduces their differentiation and migratory potential under normoxic conditions. Importantly, this phenotype comprises a tissue-specific signature as it was evident in aASCs but not in subcutaneous ASCs. NOX-induced ROS accumulation and cytokine production by fat are part of the metabolic syndrome. The similarity of this

  5. Accumulation Rates in the Dry Snow Zone of the Greenland Ice Sheet Inferred from L-band InSAR Data

    NASA Astrophysics Data System (ADS)

    Chen, A. C.; Zebker, H. A.

    2012-12-01

    The Greenland ice sheet contains about 2.9 million km3 of ice and would raise global sea levels by about 7.1 m if it melted completely. Two unusually large iceberg calving events at Petermann Glacier in the past several years, along with the unusually large extent of ice sheet melt this summer point to the relevance of understanding the mass balance of the Greenland ice sheet. In this study, we use data from the PALSAR instrument aboard the ALOS satellite to form L-band (23-centimeter carrier wavelength) InSAR images of the dry snow zone of the Greenland ice sheet. We form geocoded differential interferograms, using the ice sheet elevation model produced by Howat et.al. [1]. By applying phase and radiometric calibration, we can examine interferograms formed between any pair of transmit and receive polarization channels. In co-polarized interferograms, the InSAR correlation ranges from about 0.35 at the summit (38.7 deg W, 73.0 deg N) where accumulation is about 20 cm w.e./yr to about 0.70 at the north-eastern part of the dry snow zone (35.1 deg W, 77.1 deg N), where accumulation is about 11.7 cm w.e./yr. Cross-polarized interferograms show similar geographic variation with overall lower correlation. We compare our InSAR data with in-situ measurements published by Bales et.al. [2]. We examine the applicability of dense-medium radiative transfer electromagnetic scattering models for estimating accumulation rates from L-band InSAR data. The large number and broad coverage of ALOS scenes acquired between 2007 and 2009 with good InSAR coherence at 46-day repeat times and 21.5 degree incidence angles gives us the opportunity to examine the empirical relationship between in-situ accumulation rate observations and the polarimetric InSAR correlation and radar brightness at this particular imaging geometry. This helps us quantify the accuracy of accumulation rates estimated from InSAR data. In some regions, 46-day interferograms acquired in the winters of several consecutive

  6. Isotopic hysteresis in detrital cosmogenic nuclide-derived denudation rate studies (Invited)

    NASA Astrophysics Data System (ADS)

    Willenbring, J.; Gasparini, N. M.; Crosby, B. T.; Brocard, G. Y.; Belmont, P.

    2013-12-01

    In equilibrium landscapes, the concentration of beryllium-10 (10Be) from fluvially transported material is expected to quantitatively reflect basin-wide denudation rates. No isotopic time-dependent path is considered because the concentrations reflect an integrated measurement over a sufficiently long period of time to be static. However, the responses of landscapes to changing conditions are often addressed with cosmogenic nuclides in transient landscapes to identify and quantify the primary topographic and climatic controls on erosion. With the advent of techniques that allow event-scale measurement of cosmogenic nuclide concentrations over the course of a flood wave, in the case of meteoric 10Be, or over the course of an uplift wave, in the case of in situ-produced 10Be, we can now evaluate the how the isotope changes and what the 'mean' denudation rate from a single time means. Meteoric 10Be concentrations can be extracted from, and measured in, milligram-sized sediment samples. This attribute enables us to measure suspended sediment through a hydrograph. Here, we give a case study in an agricultural setting. The meteoric 10Be concentration in river sediment changes with the source areas of the fine sediment and fluxes of material supplied to the stream. The average concentration from the couplet of the rising and falling limbs of the hydrograph can differ from the concentration of the sediment that is preserved in depo-centers. Using traditional in situ-produced 10Be, the timescale of the perturbation must be sufficiently long to change the isotopic composition of the bedload, but also for the landscape to respond to the forcing factor. Here, we give an example from a transient landscape where a wave of uplift moves through the basin and a wave of incision follows in its wake. In this setting, 10Be from detrital quartz is derived from both the incising, adjusting lowland and the unadjusted, relict upland, and the integrated 10Be concentrations still provide a

  7. Accumulation and altered localization of telomere-associated protein TRF2 in immortally transformed and tumor-derived human breast cells

    SciTech Connect

    Nijjar, Tarlochan; Bassett, Ekaterina; Garbe, James; Takenaka, Yasuhiro; Stampfer, Martha R.; Gilley, David; Yaswen, Paul

    2004-12-23

    We have used cultured human mammary epithelial cells (HMEC) and breast tumor-derived lines to gain information on defects that occur during breast cancer progression. HMEC immortalized by a variety of agents (the chemical carcinogen benzo(a)pyrene, oncogenes c-myc and ZNF217, and/or dominant negative p53 genetic suppressor element GSE22) displayed marked up regulation (10-15 fold) of the telomere binding protein, TRF2. Up-regulation of TRF2 protein was apparently due to differences in post-transcriptional regulation, as mRNA levels remained comparable in finite life span and immortal HMEC. TRF2 protein was not up-regulated by the oncogenic agents alone in the absence of immortalization, nor by expression of exogenously introduced hTERT genes. We found TRF2 levels to be at least 2-fold higher than in control cells in 11/15 breast tumor cell lines, suggesting that elevated TRF2 levels are a frequent occurrence during the transformation of breast tumor cells in vivo. The dispersed distribution of TRF2 throughout the nuclei in some immortalized and tumor-derived cells indicated that not all the TRF2 was associated with telomeres in these cells. The process responsible for accumulation of TRF2 in immortalized HMEC and breast tumor-derived cell lines may promote tumorigenesis by contributing to the cells ability to maintain an indefinite life span.

  8. A spiroketal-enol ether derivative from Tanacetum vulgare selectively inhibits HSV-1 and HSV-2 glycoprotein accumulation in Vero cells.

    PubMed

    Álvarez, Ángel L; Habtemariam, Solomon; Abdel Moneim, Ahmed E; Melón, Santiago; Dalton, Kevin P; Parra, Francisco

    2015-07-01

    The inhibitory effects of Tanacetum vulgare rhizome extracts on HSV-1 and HSV-2 in vitro replication were assessed. Unlike extracts obtained from the aerial parts, adsorption inhibition and virucidal activities seemed not to be relevant for the observed antiviral action of tansy rhizome extracts. Instead, the most significant effects were the inhibition of virus penetration and a novel mechanism consisting of the specific arrest of viral gene expression and consequently the decrease of viral protein accumulation within infected cells. Through a bioactivity-guided fractionation protocol we isolated and identified the spiroketal-enol ether derivative (E)-2-(2,4-hexadiynyliden)-1,6-dioxaspiro[4.5]dec-3-ene as the active compound responsible for this inhibitory effect.

  9. Geologic ages and accumulation rates of basalt-flow groups and sedimentary interbeds in selected wells at the Idaho National Engineering Laboratory, Idaho

    SciTech Connect

    Anderson, S.R.; Liszewski, M.J.; Cecil, L.D.

    1997-01-01

    Geologic ages and accumulation rates, estimated from regressions, were used to evaluate measured ages and interpreted stratigraphic and structural relations of basalt and sediment in the unsaturated zone and the Snake River Plain aquifer at the Idaho National Engineering Laboratory (INEL) in eastern Idaho. Geologic ages and accumulation rates were estimated from standard linear regressions of 21 mean potassium-argon (K-Ar) ages, selected mean paleomagnetic ages, and cumulative depths of a composite stratigraphic section composed of complete intervals of basalt and sediment that were deposited in areas of past maximum subsidence. Accumulation rates also were estimated from regressions of stratigraphic intervals in three wells in and adjacent to an area of interpreted uplift at and near the Idaho Chemical Processing Plant (ICPP) and the Test Reactor Area (TRA) to allow a comparison of rates in areas of past uplift and subsidence. Estimated geologic ages range from about 200 thousand to 1.8 million years before present and are reasonable approximations for the interval of basalt and sediment above the effective base of the aquifer, based on reported uncertainties of corresponding measured ages. Estimated ages between 200 and 800 thousand years are within the range of reported uncertainties for all 15 K-Ar ages used in regressions and two out of three -argon ({sup 40}Ar/{sup 39}Ar) ages of duplicate argon samples. Two sets of estimated ages between 800 thousand and 1.8 million years are within the range of reported uncertainties for all seven K-Ar ages used in regressions, which include one shared age of about 800 thousand years. Two sets of ages were estimated for this interval because K-Ar ages make up two populations that agree with previous and revised ages of three paleomagnetic subchrons. The youngest set of ages is consistent with a K-Ar age from the effective base of the aquifer that agrees with previous ages of the Olduvai Normal-Polarity Subchron.

  10. Archean high δ18O Mg-diorite: crustal-derived melt hybridized with enriched mafic accumulated rocks

    NASA Astrophysics Data System (ADS)

    Wang, Dan; Guo, Jing-Hui

    2016-04-01

    The genesis of Mg-diorite or sanukitoids has significances to understand the crustal growth and tectonic style in Archean. The chemical compositions of minerals and rocks, whole-rock Sm-Nd isotope, zircon SIMS U-Pb ages and Hf-O isotopes of Zhulagou (ZLG) Mg-diorite and their mafic enclaves (Yinshan Block, North China Craton) were studied to place constraints on their sources and genesis, and therefore provide information about dynamic processes. The ~2520 Ma ZLG diorites have intermediate SiO2 (59.4-65.5 wt.%), high Mg# (49-52), Cr (90.4-438 ppm), Ni (15.0-95.9 ppm), Sr (436-882 ppm) and Ba (237-1206 ppm) contents with fractionated rare earth elements (REE, LaN/YbN = 9.1-40.5) and depleted high field-strength element (HFSE, e.g. Nb, Ta and Ti). These geochemical signatures are similar to those Archean high-Mg diorites and sanukitoids. However, they are sodic with low K2O/Na2O (0.14-0.49) ratios, exhibiting an affinity with Archean trondhjemite-tonalite-granodiorite (TTG). Abundant coeval amphibole-bearing mafic enclaves (~2525 Ma) are enclosed within the ZLG diorites. They display low SiO2 (46.5-50.3 wt.%) contents but high concentrations of MgO (9.0-14.5 wt.%), Cr (647-1946 ppm) and Ni (197-280 ppm). They are enriched in K2O (0.64-3.43 wt.%) and large ion lithophile element (LILE), depleted in Nb, Ta and Ti. Combined with their concave REE patterns and prominent negative Eu anomaly, we suggest that they are cumulates of the melt which probably derived from subduction-related Archean metasomatized mantle source. Mineral trace element modelling results, similar ɛNd(t) (+0.6 to +2.3) and δ18O(Zrc) values (~8.6-9.0 ‰) of the diorites and mafic enclaves, strongly reflect that they had experienced intense interaction and hybridization. Evolved whole-rock Nd isotopes (TDM = 2.80-2.70 Ga), variable zircon ɛHf (t) (-1.6 to +6.0) and high δ18O (~9.0 ‰) values of the diorites indicate that they most likely originated from melting of an older continental crust (≥ 2

  11. Leaf senescence in rice due to magnesium deficiency mediated defect in transpiration rate before sugar accumulation and chlorosis.

    PubMed

    Kobayashi, Natsuko I; Saito, Takayuki; Iwata, Naoko; Ohmae, Yoshimi; Iwata, Ren; Tanoi, Keitaro; Nakanishi, Tomoko M

    2013-08-01

    Magnesium (Mg) is an essential macronutrient supporting various functions, including photosynthesis. However, the specific physiological responses to Mg deficiency remain elusive. In this study, 2-week-old rice seedlings (Oryza sativa. cv. Nipponbare) with three expanded leaves (L2-L4) were transferred to Mg-free nutrient solution for 8 days. In the absence of Mg, on day 8, L5 and L6 were completely developed, while L7 just emerged. We also studied several mineral deficiencies to identify specific responses to Mg deficiency. Each leaf was analyzed in terms of chlorophyll, starch, anthocyanin and carbohydrate metabolites, and only absence of Mg was found to cause irreversible senescence of L5. Resupply of Mg at various time points confirmed that the borderline of L5 death was between days 6 and 7 of Mg deficiency treatment. Decrease in chlorophyll concentration and starch accumulation occurred simultaneously in L5 and L6 blades on day 8. However, nutrient transport drastically decreased in L5 as early as day 6. These data suggest that the predominant response to Mg deficiency is a defect in transpiration flow. Furthermore, changes in myo-inositol and citrate concentrations were detected only in L5 when transpiration decreased, suggesting that they may constitute new biological markers of Mg deficiency.

  12. Leaf senescence in rice due to magnesium deficiency mediated defect in transpiration rate before sugar accumulation and chlorosis.

    PubMed

    Kobayashi, Natsuko I; Saito, Takayuki; Iwata, Naoko; Ohmae, Yoshimi; Iwata, Ren; Tanoi, Keitaro; Nakanishi, Tomoko M

    2013-08-01

    Magnesium (Mg) is an essential macronutrient supporting various functions, including photosynthesis. However, the specific physiological responses to Mg deficiency remain elusive. In this study, 2-week-old rice seedlings (Oryza sativa. cv. Nipponbare) with three expanded leaves (L2-L4) were transferred to Mg-free nutrient solution for 8 days. In the absence of Mg, on day 8, L5 and L6 were completely developed, while L7 just emerged. We also studied several mineral deficiencies to identify specific responses to Mg deficiency. Each leaf was analyzed in terms of chlorophyll, starch, anthocyanin and carbohydrate metabolites, and only absence of Mg was found to cause irreversible senescence of L5. Resupply of Mg at various time points confirmed that the borderline of L5 death was between days 6 and 7 of Mg deficiency treatment. Decrease in chlorophyll concentration and starch accumulation occurred simultaneously in L5 and L6 blades on day 8. However, nutrient transport drastically decreased in L5 as early as day 6. These data suggest that the predominant response to Mg deficiency is a defect in transpiration flow. Furthermore, changes in myo-inositol and citrate concentrations were detected only in L5 when transpiration decreased, suggesting that they may constitute new biological markers of Mg deficiency. PMID:23176135

  13. Escherichia coli-Derived Uracil Increases the Antibacterial Activity and Growth Rate of Lactobacillus plantarum.

    PubMed

    Ha, Eun-Mi

    2016-05-28

    Lactobacillus plantarum (L. plantarum) is a representative probiotic. In particular, L. plantarum is the first commensal bacterium to colonize the intestine of infants. For this reason, the initial settlement of L. plantarum can play an important role in determining an infant's health as well as their eventual health status as an adult. In addition, L. plantarum combats pathogenic infections (such as Escherichia coli (E. coli), one of the early pathogenic colonizers in an unhealthy infant gut) by secreting antimicrobial substances. The aim of this research was to determine how L. plantarum combats E. coli infection and why it is a representative probiotic in the intestine. Consequently, this research observed that E. coli releases uracil. L. plantarum specifically recognizes E. coli-derived uracil, which increases the growth rate and production of antimicrobial substance of L. plantarum. In addition, through the inhibitory activity test, this study postulates that the antimicrobial substance is a protein and can be considered a bacteriocin-like substance. Therefore, this research assumes that L. plantarum exerts its antibacterial ability by recognizing E. coli and increasing its growth rate as a result, and this phenomenon could be one of the reasons for L. plantarum settling in the intestine of infants as a beneficial bacterium. PMID:27012237

  14. Effects of feeding and organism loading rate on PCB accumulation by Lumbriculus variegatus in sediment bioaccumulation testing

    EPA Science Inventory

    Sediment bioaccumulation test methods published by USEPA and ASTM in 2000 specify that the Lumbriculus variegatus, a freshwater oligochaete, should not be fed during the 28-day exposure and recommends an organism loading rate of total organic carbon in sediment to organism dry we...

  15. The null hypothesis: steady rates of erosion, weathering and sediment accumulation during Late Cenozoic mountain uplift and glaciation

    NASA Astrophysics Data System (ADS)

    Willenbring, J. K.; Jerolmack, D. J.

    2015-12-01

    At the largest time and space scales, the pace of erosion and chemical weathering is determined by tectonic uplift rates. Deviations from this equilibrium condition arise from the transient response of landscape denudation to climatic and tectonic perturbations, and may be long lived. We posit that the constraint of mass balance, however, makes it unlikely that such disequilibrium persists at the global scale over millions of years, as has been proposed for late Cenozoic erosion. To support this contention, we synthesize existing data for weathering fluxes, global sedimentation rates, sediment yields and tectonic motions. The records show a remarkable constancy in the pace of Earth-surface evolution over the last 10 million years. These findings provide strong support for the null hypothesis; that global rates of landscape change have remained constant over the last ten million years, despite global climate change and massive mountain building events. Two important implications are: (1) global climate change may not change global denudation rates, because the nature and sign of landscape responses are varied; and (2) tectonic and climatic perturbations are accommodated in the long term by changes in landscape form. This work undermines the hypothesis that increased weathering due to late Cenozoic mountain building or climate change was the primary agent for a decrease in global temperatures.

  16. Urban rivers as conveyors of hydrocarbons to sediments of estuarine areas: source characterization, flow rates and mass accumulation.

    PubMed

    Mauad, Cristiane R; Wagener, Angela de L R; Massone, Carlos G; Aniceto, Mayara da S; Lazzari, Letícia; Carreira, Renato S; Farias, Cássia de O

    2015-02-15

    Aliphatic (n-C12-n-C40, unresolved complex mixture, resolved peaks) and aromatic hydrocarbons (46 PAH) were investigated in suspended particulate matter (SPM) sampled over eleven months in six of the major rivers and two channels of the Guanabara Bay Basin. PAH flow rates of the most contaminated rivers, the contribution to the PAH sediment load of the receiving bay, and the main sources of hydrocarbons were determined. PAH (38) ranged from 28 ng L(-1) to 11,514 ng L(-1). Hydrocarbon typology and statistical evaluation demonstrated contribution of distinct sources in different regions and allowed quantification of these contributions. Total flow rate for the five major rivers amounts to 3 t year(-1) and responds for 30% of the total PAH annual input into the northern area of the Guanabara Bay. For the first time PAH mass deposited in the bay sediments has been estimated and shall serve as base for decision making and source abatement.

  17. Influence of Light and Temperature on Gene Expression Leading to Accumulation of Specific Flavonol Glycosides and Hydroxycinnamic Acid Derivatives in Kale (Brassica oleracea var. sabellica).

    PubMed

    Neugart, Susanne; Krumbein, Angelika; Zrenner, Rita

    2016-01-01

    Light intensity and temperature are very important signals for the regulation of plant growth and development. Plants subjected to less favorable light or temperature conditions often respond with accumulation of secondary metabolites. Some of these metabolites have been identified as bioactive compounds, considered to exert positive effects on human health when consumed regularly. In order to test a typical range of growth parameters for the winter crop Brassica oleracea var. sabellica, plants were grown either at 400 μmol m(-2) s(-1) or 100 μmol m(-2) s(-1) at 10°C, or at 400 μmol m(-2) s(-1) with 5 or 15°C. The higher light intensity overall increased flavonol content of leaves, favoring the main quercetin glycosides, a caffeic acid monoacylated kaempferol triglycoside, and disinapoyl-gentiobiose. The higher temperature mainly increased the hydroxycinnamic acid derivative disinapoyl-gentiobiose, while at lower temperature synthesis is in favor of very complex sinapic acid acylated flavonol tetraglycosides such as kaempferol-3-O-sinapoyl-sophoroside-7-O-diglucoside. A global analysis of light and temperature dependent alterations of gene expression in B. oleracea var. sabellica leaves was performed with the most comprehensive Brassica microarray. When compared to the light experiment much less genes were differentially expressed in kale leaves grown at 5 or 15°C. A structured evaluation of differentially expressed genes revealed the expected enrichment in the functional categories of e.g. protein degradation at different light intensities or phytohormone metabolism at different temperature. Genes of the secondary metabolism namely phenylpropanoids are significantly enriched with both treatments. Thus, the genome of B. oleracea was screened for predicted genes putatively involved in the biosynthesis of flavonoids and hydroxycinnamic acid derivatives. All identified B. oleracea genes were analyzed for their most specific 60-mer oligonucleotides present on the

  18. Influence of Light and Temperature on Gene Expression Leading to Accumulation of Specific Flavonol Glycosides and Hydroxycinnamic Acid Derivatives in Kale (Brassica oleracea var. sabellica).

    PubMed

    Neugart, Susanne; Krumbein, Angelika; Zrenner, Rita

    2016-01-01

    Light intensity and temperature are very important signals for the regulation of plant growth and development. Plants subjected to less favorable light or temperature conditions often respond with accumulation of secondary metabolites. Some of these metabolites have been identified as bioactive compounds, considered to exert positive effects on human health when consumed regularly. In order to test a typical range of growth parameters for the winter crop Brassica oleracea var. sabellica, plants were grown either at 400 μmol m(-2) s(-1) or 100 μmol m(-2) s(-1) at 10°C, or at 400 μmol m(-2) s(-1) with 5 or 15°C. The higher light intensity overall increased flavonol content of leaves, favoring the main quercetin glycosides, a caffeic acid monoacylated kaempferol triglycoside, and disinapoyl-gentiobiose. The higher temperature mainly increased the hydroxycinnamic acid derivative disinapoyl-gentiobiose, while at lower temperature synthesis is in favor of very complex sinapic acid acylated flavonol tetraglycosides such as kaempferol-3-O-sinapoyl-sophoroside-7-O-diglucoside. A global analysis of light and temperature dependent alterations of gene expression in B. oleracea var. sabellica leaves was performed with the most comprehensive Brassica microarray. When compared to the light experiment much less genes were differentially expressed in kale leaves grown at 5 or 15°C. A structured evaluation of differentially expressed genes revealed the expected enrichment in the functional categories of e.g. protein degradation at different light intensities or phytohormone metabolism at different temperature. Genes of the secondary metabolism namely phenylpropanoids are significantly enriched with both treatments. Thus, the genome of B. oleracea was screened for predicted genes putatively involved in the biosynthesis of flavonoids and hydroxycinnamic acid derivatives. All identified B. oleracea genes were analyzed for their most specific 60-mer oligonucleotides present on the

  19. Influence of Light and Temperature on Gene Expression Leading to Accumulation of Specific Flavonol Glycosides and Hydroxycinnamic Acid Derivatives in Kale (Brassica oleracea var. sabellica)

    PubMed Central

    Neugart, Susanne; Krumbein, Angelika; Zrenner, Rita

    2016-01-01

    Light intensity and temperature are very important signals for the regulation of plant growth and development. Plants subjected to less favorable light or temperature conditions often respond with accumulation of secondary metabolites. Some of these metabolites have been identified as bioactive compounds, considered to exert positive effects on human health when consumed regularly. In order to test a typical range of growth parameters for the winter crop Brassica oleracea var. sabellica, plants were grown either at 400 μmol m−2 s−1 or 100 μmol m−2 s−1 at 10°C, or at 400 μmol m−2 s−1 with 5 or 15°C. The higher light intensity overall increased flavonol content of leaves, favoring the main quercetin glycosides, a caffeic acid monoacylated kaempferol triglycoside, and disinapoyl-gentiobiose. The higher temperature mainly increased the hydroxycinnamic acid derivative disinapoyl-gentiobiose, while at lower temperature synthesis is in favor of very complex sinapic acid acylated flavonol tetraglycosides such as kaempferol-3-O-sinapoyl-sophoroside-7-O-diglucoside. A global analysis of light and temperature dependent alterations of gene expression in B. oleracea var. sabellica leaves was performed with the most comprehensive Brassica microarray. When compared to the light experiment much less genes were differentially expressed in kale leaves grown at 5 or 15°C. A structured evaluation of differentially expressed genes revealed the expected enrichment in the functional categories of e.g. protein degradation at different light intensities or phytohormone metabolism at different temperature. Genes of the secondary metabolism namely phenylpropanoids are significantly enriched with both treatments. Thus, the genome of B. oleracea was screened for predicted genes putatively involved in the biosynthesis of flavonoids and hydroxycinnamic acid derivatives. All identified B. oleracea genes were analyzed for their most specific 60-mer oligonucleotides present on the

  20. Magnetically Derived Flood Recurrence Rate Estimates from Stalagmites in Southeastern Minnesota

    NASA Astrophysics Data System (ADS)

    Feinberg, J. M.; Lascu, I.; Andrade Lima, E.; Weiss, B. P.

    2012-12-01

    The magnetism of speleothems remains an untapped resource of paleoclimatic, hydrogeologic, and geomagnetic information. Similar to other deposits containing magnetic minerals, speleothems chronicle the evolution of local environmental parameters via the concentration, composition and grain size of their magnetic mineral assemblages. Here we report a novel use of scanning SQUID microscopy to calculate flood recurrence rates from an annually laminated ~500 year old stalagmite from Spring Valley Caverns (SVC) in southeastern Minnesota. Mineral and organic detritus adheres to the surface of a speleothem as flood waters recede from a cavern, and are subsequently encapsulated by calcite as drip water conditions are reestablished. Such detritus typically consists of allochthonous grains of quartz, clay, and titanomagnetite with an average grain size of ~10 μm. Larger flood layers occur on polished surfaces as dark bands that delineate stalagmite growth horizons. We use scanning SQUID microscopy (with a nominal sensitivity of 10-16 Am2) to map the presence of these flood layers by measuring the vertical component of the stray magnetic field resulting from a 1 T isothermal remanent magnetization (IRM) imparted perpendicular to a polished surface. A magnetization model of the IRM field was then obtained by inverting the field data measured 210 μm above the sample using an algorithm in the Fourier domain. By integrating the magnetic data parallel to the stalagmite growth axis we produce a time series of IRM peaks, each of which corresponds to a flooding event. We calculate an average flood recurrence rate of 5 per century for the last 500 years. This rate increases to >10 floods per century in the last century, thereby capturing the combined effects of both climate change and agricultural land-use on karst hydrogeology. These results agree with recurrence rate estimates derived from historical records, tree ring studies, and geochemical analyses of speleothems. The presence

  1. Urban rivers as conveyors of hydrocarbons to sediments of estuarine areas: source characterization, flow rates and mass accumulation.

    PubMed

    Mauad, Cristiane R; Wagener, Angela de L R; Massone, Carlos G; Aniceto, Mayara da S; Lazzari, Letícia; Carreira, Renato S; Farias, Cássia de O

    2015-02-15

    Aliphatic (n-C12-n-C40, unresolved complex mixture, resolved peaks) and aromatic hydrocarbons (46 PAH) were investigated in suspended particulate matter (SPM) sampled over eleven months in six of the major rivers and two channels of the Guanabara Bay Basin. PAH flow rates of the most contaminated rivers, the contribution to the PAH sediment load of the receiving bay, and the main sources of hydrocarbons were determined. PAH (38) ranged from 28 ng L(-1) to 11,514 ng L(-1). Hydrocarbon typology and statistical evaluation demonstrated contribution of distinct sources in different regions and allowed quantification of these contributions. Total flow rate for the five major rivers amounts to 3 t year(-1) and responds for 30% of the total PAH annual input into the northern area of the Guanabara Bay. For the first time PAH mass deposited in the bay sediments has been estimated and shall serve as base for decision making and source abatement. PMID:25434473

  2. COMPARISON OF IN VIVO DERIVED AND SCALED IN VITRO METABOLIC RATE CONSTANTS FOR SOME VOLATILE ORGANIC COMPOUNDS (VOCS)

    EPA Science Inventory

    The reliability of physiologically based pharmacokinetic (PBPK) models is directly related to the accuracy of the metabolic rate parameters used as model inputs. When metabolic rate parameters derived from in vivo experiments are unavailable, they can be estimated from in vitro d...

  3. Denudation rates determined from the accumulation of in situ-produced 10Be in the luquillo experimental forest, Puerto Rico

    USGS Publications Warehouse

    Brown, Erik Thorson; Stallard, Robert F.; Larsen, Matthew C.; Raisbeck, Grant M.; Yiou, Francoise

    1995-01-01

    We present a simple method for estimation of long-term mean denudation rates using in situ-produced cosmogenic 10Be in fluvial sediments. Procedures are discussed to account for the effects of soil bioturbation, mass wasting and attenuation of cosmic rays by biomass and by local topography. Our analyses of 10Be in quartz from bedrock outcrops, soils, mass-wasting sites and riverine sediment from the Icacos River basin in the Luquillo Experimental Forest, Puerto Rico, are used to characterize denudation for major landform elements in that basin. The 10Be concentration of a discharge-weighted average of size classes of river sediment corresponds to a long-term average denudation of ≈ 43 m Ma −1, consistent with mass balance results. 

  4. Acoustically derived growth rates of sperm whales (Physeter macrocephalus) in Kaikoura, New Zealand.

    PubMed

    Miller, Brian S; Growcott, Abraham; Slooten, Elisabeth; Dawson, Stephen M

    2013-09-01

    A non-invasive acoustic method for measuring the growth of sperm whales was developed based on estimating the length of individuals by measuring the inter-pulse interval (IPI) of their clicks. Most prior knowledge of growth in male sperm whales has come from from fitting growth curves to length data gained from whaling. Recordings made at Kaikoura, New Zealand, were used to estimate the length and growth of 32 photographically identified, resident whales that have been recorded repeatedly between 1991 and 2009. All whales recorded more than six months apart (n = 30) showed an increase in IPI. Using established relationships between IPI and total length, it was found that the average growth rate in the Kaikoura population is lower, especially for smaller whales (13-14.5 m), than that derived from historical whaling data from other populations. This difference may be due to ecological differences among populations but might also reflect upward bias in measurements gained in whaling. The ability to track growth of individuals through time is only possible via non-lethal means and offers a fundamentally different kind of data because differences among individuals can be measured.

  5. Hydrohalite in cold sea ice: Laboratory observations of single crystals, surface accumulations, and migration rates under a temperature gradient, with application to “Snowball Earth”

    NASA Astrophysics Data System (ADS)

    Light, Bonnie; Brandt, Richard E.; Warren, Stephen G.

    2009-07-01

    When NaCl precipitates out of a saturated solution, it forms anhydrous crystals of halite at temperatures above +0.11°C, but at temperatures below this threshold it instead precipitates as the dihydrate "hydrohalite," NaCl · 2H2O. When sea ice is cooled, hydrohalite begins to precipitate within brine inclusions at about -23°C. In this work, hydrohalite crystals are examined in laboratory experiments: their formation, their shape, and their response to warming and desiccation. Sublimation of a sea ice surface at low temperature leaves a lag deposit of hydrohalite, which has the character of a fine powder. The precipitation of hydrohalite in brine inclusions raises the albedo of sea ice, and the subsequent formation of a surface accumulation further raises the albedo. Although these processes have limited climatic importance on the modern Earth, they would have been important in determining the surface types present in regions of net sublimation on the tropical ocean in the cold phase of a Snowball Earth event. However, brine inclusions in sea ice migrate downward to warmer ice, so whether salt can accumulate on the surface depends on the relative rates of sublimation and migration. The migration rates are measured in a laboratory experiment at temperatures from -2°C to -32°C; the migration appears to be too slow to prevent formation of a salt crust on Snowball Earth.

  6. Examination of sludge accumulation rates and sludge characteristics for a decentralized community wastewater treatment systems with individual primary clarifier tanks located in Wardsville (Ontario, Canada).

    PubMed

    Lossing, Heather; Champagne, Pascale; McLellan, P James

    2010-01-01

    In conventional septic systems, settling and partial treatment via anaerobic digestion occurs in the septic tank. One of the byproducts of solids separation in the septic tank is a semi-liquid material known as septage, which must be periodically pumped out. Septage includes the liquid portion within the tank, as well as the sludge that settles at the bottom of the tank and the scum that floats to the surface of the liquid layer. A number of factors can influence septage characteristics, as well as the sludge and scum accumulation rates within the tank. This paper presents the results of a 2007 field sampling study conducted in Wardsville (Ontario, Canada). The field study examined 29 individual residential two-chamber septic tanks in a community serviced by a decentralized wastewater treatment system in operation for approximately 7 years without septage removal. The field investigation provided a comprehensive data set that allowed for statistical analysis of the data to assess the more critical factors influencing solids accumulation rates within each of the clarifier chambers. With this data, a number of predictive models were developed using water usage data for each residence as an explanatory variable.

  7. Early and long-term mantle processing rates derived from xenon isotopes

    NASA Astrophysics Data System (ADS)

    Mukhopadhyay, S.; Parai, R.; Tucker, J.; Middleton, J. L.; Langmuir, C. H.

    2015-12-01

    Noble gases, particularly xenon (Xe), in mantle-derived basalts provide a rich portrait of mantle degassing and surface-interior volatile exchange. The combination of extinct and extant radioactive species in the I-Pu-U-Xe systems shed light on the degassing history of the early Earth throughout accretion, as well as the long-term degassing of the Earth's interior in association with plate tectonics. The ubiquitous presence of shallow-level air contamination, however, frequently obscures the mantle Xe signal. In a majority of the samples, shallow air contamination dominates the Xe budget. For example, in the gas-rich popping rock 2ΠD43, 129Xe/130Xe ratios reach 7.7±0.23 in individual step-crushes, but the bulk composition of the sample is close to air (129Xe/130Xe of 6.7). Thus, the extent of variability in mantle source Xe composition is not well-constrained. Here, we present new MORB Xe data and explore constraints placed on mantle processing rates by the Xe data. Ten step-crushes were obtained on a depleted popping glass that was sealed in ultrapure N2 after dredge retrieval from between the Kane-Atlantis Fracture Zone of the Mid Atlantic Ridge in May 2012. 9 steps yielded 129Xe/130Xe of 7.50-7.67 and one yielded 7.3. The bulk 129Xe/130Xe of the sample is 7.6, nearly identical to the estimated mantle source value of 7.7 for the sample. Hence, the sample is virtually free of shallow-level air contamination. Because sealing the sample in N2upon dredge retrieval largely eliminated air contamination, for many samples, contamination must be added after sample retrieval from the ocean bottom. Our new high-precision Xe isotopic measurements in upper mantle-derived samples provide improved constraints on the Xe isotopic composition of the mantle source. We developed a forward model of mantle volatile evolution to identify solutions that satisfy our Xe isotopic data. We find that accretion timescales of ~10±5 Myr are consistent with I-Pu-Xe constraints, and the last

  8. Estimating the effects of 17α-ethinylestradiol on stochastic population growth rate of fathead minnows: a population synthesis of empirically derived vital rates.

    PubMed

    Schwindt, Adam R; Winkelman, Dana L

    2016-09-01

    Urban freshwater streams in arid climates are wastewater effluent dominated ecosystems particularly impacted by bioactive chemicals including steroid estrogens that disrupt vertebrate reproduction. However, more understanding of the population and ecological consequences of exposure to wastewater effluent is needed. We used empirically derived vital rate estimates from a mesocosm study to develop a stochastic stage-structured population model and evaluated the effect of 17α-ethinylestradiol (EE2), the estrogen in human contraceptive pills, on fathead minnow Pimephales promelas stochastic population growth rate. Tested EE2 concentrations ranged from 3.2 to 10.9 ng L(-1) and produced stochastic population growth rates (λ S ) below 1 at the lowest concentration, indicating potential for population decline. Declines in λ S compared to controls were evident in treatments that were lethal to adult males despite statistically insignificant effects on egg production and juvenile recruitment. In fact, results indicated that λ S was most sensitive to the survival of juveniles and female egg production. More broadly, our results document that population model results may differ even when empirically derived estimates of vital rates are similar among experimental treatments, and demonstrate how population models integrate and project the effects of stressors throughout the life cycle. Thus, stochastic population models can more effectively evaluate the ecological consequences of experimentally derived vital rates. PMID:27372448

  9. Mast cell-deficient Kit(W-sh) "Sash" mutant mice display aberrant myelopoiesis leading to the accumulation of splenocytes that act as myeloid-derived suppressor cells.

    PubMed

    Michel, Anastasija; Schüler, Andrea; Friedrich, Pamela; Döner, Fatma; Bopp, Tobias; Radsak, Markus; Hoffmann, Markus; Relle, Manfred; Distler, Ute; Kuharev, Jörg; Tenzer, Stefan; Feyerabend, Thorsten B; Rodewald, Hans-Reimer; Schild, Hansjörg; Schmitt, Edgar; Becker, Marc; Stassen, Michael

    2013-06-01

    Mast cell-deficient Kit(W-sh) "sash" mice are widely used to investigate mast cell functions. However, mutations of c-Kit also affect additional cells of hematopoietic and nonimmune origin. In this study, we demonstrate that Kit(W-sh) causes aberrant extramedullary myelopoiesis characterized by the expansion of immature lineage-negative cells, common myeloid progenitors, and granulocyte/macrophage progenitors in the spleen. A consistent feature shared by these cell types is the reduced expression of c-Kit. Populations expressing intermediate and high levels of Ly6G, a component of the myeloid differentiation Ag Gr-1, are also highly expanded in the spleen of sash mice. These cells are able to suppress T cell responses in vitro and phenotypically and functionally resemble myeloid-derived suppressor cells (MDSC). MDSC typically accumulate in tumor-bearing hosts and are able to dampen immune responses. Consequently, transfer of MDSC from naive sash mice into line 1 alveolar cell carcinoma tumor-bearing wild-type littermates leads to enhanced tumor progression. However, although it can also be observed in sash mice, accelerated growth of transplanted line 1 alveolar cell carcinoma tumors is a mast cell-independent phenomenon. Thus, the Kit(W-sh) mutation broadly affects key steps in myelopoiesis that may have an impact on mast cell research. PMID:23636054

  10. Alternative to the steady-state method: derivation of reaction rates from first-passage times and pathway probabilities.

    PubMed Central

    Ninio, J

    1987-01-01

    An alternative method for deriving rate equations in enzyme kinetics is presented. An enzyme is followed as it moves along the various pathways allowed by the reaction scheme. The times spent in various sections of the scheme and the pathway probabilities are computed, using simple rules. The rate equation obtains as a function of times and probabilities. The results are equivalent to those provided by the steady-state formalism. While the latter applies uniformly to all schemes, the formalism presented here requires adaptation to each additional class of schemes. However, it has the merit of allowing one to leave unspecified many details of the scheme, including topological ones. Furthermore, it allows one to decompose a scheme into subschemes, analyze the parts separately, and use the intermediate results to derive the rate equation of the complete scheme. The method is applied here to derive general equations for one- and two-entry site enzymes. PMID:3468503

  11. Accumulate-Repeat-Accumulate-Accumulate Codes

    NASA Technical Reports Server (NTRS)

    Divsalar, Dariush; Dolinar, Samuel; Thorpe, Jeremy

    2007-01-01

    Accumulate-repeat-accumulate-accumulate (ARAA) codes have been proposed, inspired by the recently proposed accumulate-repeat-accumulate (ARA) codes. These are error-correcting codes suitable for use in a variety of wireless data-communication systems that include noisy channels. ARAA codes can be regarded as serial turbolike codes or as a subclass of low-density parity-check (LDPC) codes, and, like ARA codes they have projected graph or protograph representations; these characteristics make it possible to design high-speed iterative decoders that utilize belief-propagation algorithms. The objective in proposing ARAA codes as a subclass of ARA codes was to enhance the error-floor performance of ARA codes while maintaining simple encoding structures and low maximum variable node degree.

  12. Glaciation in the Late Noachian Icy Highlands: Ice accumulation, distribution, flow rates, basal melting, and top-down melting rates and patterns

    NASA Astrophysics Data System (ADS)

    Fastook, James L.; Head, James W.

    2015-02-01

    Geological evidence for extensive non-polar ice deposits of Amazonian age indicates that the current cold and dry climate of Mars has persisted for several billion years. The geological record and climate history of the Noachian, the earliest period of Mars history, is less certain, but abundant evidence for fluvial channels (valley networks) and lacustrine environments (open-basin lakes) has been interpreted to represent warm and wet conditions, including rainfall and runoff. Alternatively, recent atmospheric modeling results predict a "cold and icy" Late Noachian Mars in which moderate atmospheric pressure accompanied by a full water cycle produce an atmosphere where temperature declines with elevation following an adiabatic lapse rate, in contrast to the current situation on Mars, where temperature is almost completely determined by latitude. These results are formulated in the Late Noachian Icy Highlands (LNIH) model, in which these cold and icy conditions lead to the preferential deposition of snow and ice at high elevations, such as the southern uplands. What is the fate of this snow and ice and the nature of glaciation in such an environment? What are the prospects of melting of these deposits contributing to the observed fluvial and lacustrine deposits? To address these questions, we report on a glacial flow-modeling analysis using a Mars-adapted ice sheet model with LNIH climate conditions. The total surface/near-surface water inventory is poorly known for the Late Noachian, so we explore the LNIH model in a "supply-limited" scenario for a range of available water abundances and a range of Late Noachian geothermal fluxes. Our results predict that the Late Noachian icy highlands (above an equilibrium line altitude of approximately +1 km) were characterized by extensive ice sheets of the order of hundreds of meters thick. Due to extremely cold conditions, the ice-flow velocities in general were very low, less than a few mm/yr, and the regional ice

  13. Adenylyl cyclase activation underlies intracellular cyclic AMP accumulation, cyclic AMP transport, and extracellular adenosine accumulation evoked by beta-adrenergic receptor stimulation in mixed cultures of neurons and astrocytes derived from rat cerebral cortex.

    PubMed

    Rosenberg, P A; Li, Y

    1995-09-18

    We have previously shown that stimulation of cortical cultures containing both neurons and astrocytes with the beta-adrenergic agonist isoproterenol (ISO) results in transport of cAMP from astrocytes followed by extracellular hydrolysis to adenosine [Rosenberg et al. J. Neurosci. 14 (1994) 2953-2965]. In this study we found that the endogenous catecholamines epinephrine (EPI) and norepinephrine (NE), but not dopamine, serotonin, or histamine, all at 10 microM, significantly stimulated intracellular cAMP accumulation, cAMP transport, and extracellular adenosine accumulation in cortical cultures. Detailed dose-response experiments were performed for NE and EPI, as well as ISO. For each catecholamine, the potencies in evoking intracellular cAMP accumulation, cAMP transport, and extracellular adenosine accumulation were similar. These data provide additional evidence that a single common mechanism, namely beta-adrenergic mediated activation of adenylyl cyclase, underlies intracellular cAMP accumulation, cAMP transport, and extracellular adenosine accumulation. It appears that regulation of extracellular adenosine levels via cAMP transport and extracellular hydrolysis to adenosine may be a final common pathway of neuromodulation in cerebral cortex for catecholamines, and, indeed, any substance whose receptors are coupled to adenylyl cyclase.

  14. Lava Discharge Rates at Kīlauea Volcano, Hawai'I, during 2011-2013 Determined from Tandem-X-Derived Topographic Data

    NASA Astrophysics Data System (ADS)

    Poland, M. P.

    2014-12-01

    The effusion rate of lava from a basaltic volcano is a parameter of critical importance given its direct association with hazard—for example, high effusion rates imply long lava flows. At Kīlauea Volcano, Hawai'i, numerous methods have been used to quantify effusion rate, including direct observation of lava streams, measurement of gas emissions, geologic mapping, modeling of thermal radiance, and quantification of topographic change. None of these techniques, however, have consistently yielded reliable results since about 2008, due to either changes in the character of volcanism or the expense associated with data collection. Synthetic Aperture Radar (SAR) data from the TanDEM-X satellite mission offer a potential solution to this problem. Differencing digital elevation models (DEMs) derived from temporally sequential TanDEM-X SAR imagery provides measure of elevation change over time due to accumulation of lava across the entirety of Kīlauea's 100 km2 East Rift Zone lava flow field. Summing these elevation changes over the area of an active lava flow and dividing by the time spanned by the TanDEM-X data gives the time-averaged discharge rate (TADR) of lava. The TADR calculated from multiple TanDEM-X-derived DEMs spanning days to weeks at Kīlauea during mid-2011 to mid-2013 suggests a dense-rock equivalent lava discharge rate of approximately 2 m3/s, which is about half the long-term average rate over the course of Kīlauea's 1983-present East Rift Zone eruption. This result implies an increase in the rate of lava storage at Kīlauea, a decrease in the magma supply rate, or some combination of both with respect to previous years. TanDEM-X-derived topographic maps also provide insights into the four-dimensional growth of Kīlauea's lava flow field—a dataset not available by other means but important for assessing the factors that control current and future lava flow pathways.

  15. Room temperature strain rate sensitivity in precursor derived HfO{sub 2}/Si-C-N(O) ceramic nanocomposites

    SciTech Connect

    Sujith, Ravindran; Kumar, Ravi

    2014-01-15

    Investigation on the room temperature strain rate sensitivity using depth sensing nanoindentation is carried out on precursor derived HfO{sub 2}/Si-C-N(O) ceramic nanocomposite sintered using pulsed electric current sintering. Using constant load method the strain rate sensitivity values are estimated. Lower strain rate sensitivity of ∼ 3.7 × 10{sup −3} is observed and the limited strain rate sensitivity of these ceramic nanocomposites is explained in terms of cluster model. It is concluded that presence of amorphous Si-C-N(O) clusters are responsible for the limited flowability in these ceramics.

  16. Biomass accumulation rates of Amazonian secondary forest and biomass of old-growth forests from Landsat time series and the Geoscience Laser Altimeter System

    NASA Astrophysics Data System (ADS)

    Helmer, Eileen H.; Lefsky, Michael A.; Roberts, Dar A.

    2009-01-01

    We estimate the age of humid lowland tropical forests in Rondônia, Brazil, from a somewhat densely spaced time series of Landsat images (1975-2003) with an automated procedure, the Threshold Age Mapping Algorithm (TAMA), first described here. We then estimate a landscape-level rate of aboveground woody biomass accumulation of secondary forest by combining forest age mapping with biomass estimates from the Geoscience Laser Altimeter System (GLAS). Though highly variable, the estimated average biomass accumulation rate of 8.4 Mg ha-1 yr-1 agrees well with ground-based studies for young secondary forests in the region. In isolating the lowland forests, we map land cover and general types of old-growth forests with decision tree classification of Landsat imagery and elevation data. We then estimate aboveground live biomass for seven classes of old-growth forest. TAMA is simple, fast, and self-calibrating. By not using between-date band or index differences or trends, it requires neither image normalization nor atmospheric correction. In addition, it uses an approach to map forest cover for the self-calibrations that is novel to forest mapping with satellite imagery; it maps humid secondary forest that is difficult to distinguish from old-growth forest in single-date imagery; it does not assume that forest age equals time since disturbance; and it incorporates Landsat Multispectral Scanner imagery. Variations on the work that we present here can be applied to other forested landscapes. Applications that use image time series will be helped by the free distribution of coregistered Landsat imagery, which began in December 2008, and of the Ice Cloud and land Elevation Satellite Vegetation Product, which simplifies the use of GLAS data. Finally, we demonstrate here for the first time how the optical imagery of fine spatial resolution that is viewable on Google Earth provides a new source of reference data for remote sensing applications related to land cover.

  17. alpha,beta-Dehydro-3,4-dihydroxyphenylalanine derivatives: rate and mechanism of formation.

    PubMed

    Rzepecki, L M; Waite, J H

    1991-02-15

    The amino acid L-3,4-dihydroxyphenylalanine (DOPA), when present in the primary sequence of proteins, does not form melanin upon oxidation to the quinone, since its amine moiety participates in a peptide bond and cannot undergo internal cyclization. Instead, peptidyl DOPA quinone is available for other reactions. We have investigated the oxidation chemistry of a low molecular weight peptidyl DOPA analog, N-acetylDOPA ethyl ester (NAcDEE), and have shown that a major product of oxidation is an unsaturated DOPA derivative, N-acetyl-alpha,beta-dehydroDOPA ethyl ester (NAc delta DEE) (see companion paper, Rzepecki et al., Arch. Biochem. Biophys. (1991) 285, 17-26). In the present study, we have explored kinetic and mechanistic features of the conversion of NAcDEE to NAc delta DEE and found that the reaction requires: (i) oxidation of NAcDEE to the quinone, (ii) the presence of a Lewis base as a catalyst (phosphate anion was the best of those tried in the pH range 6.0-8.0), and (iii) prevention of competing reactions such as Michael additions. Conversion efficiencies in the presence of Lewis bases ranged between 12 and 19% at pH 8.0 and 35 and 90% at pH 6.0. At least two separate reaction mechanisms appeared necessary to explain the kinetic data: (i) a pseudo-first-order mechanism at pH 6.0 and above, and (ii) an additional second-order mechanism at higher pH which involved both NAcDEE catechol and quinone. The apparent pseudo-first-order rate constants increased with pH from 2.36 X 10(-4) s-1 at pH 6.0 to about 30 X 10(-4) s-1 at pH 8.0 in 0.1 M sodium phosphate. Tautomerization of DOPA quinone to dehydroDOPA may thus be a factor in the sclerotization of natural structures incorporating DOPA containing proteins. PMID:1899328

  18. Solar Energy Deposition Rates in the Mesosphere Derived from Airglow Measurements: Implications for the Ozone Model Deficit Problem

    NASA Technical Reports Server (NTRS)

    Mlynczak, Martin G.; Garcia, Rolando R.; Roble, Raymond G.; Hagan, Maura

    2000-01-01

    We derive rates of energy deposition in the mesosphere due to the absorption of solar ultraviolet radiation by ozone. The rates are derived directly from measurements of the 1.27-microns oxygen dayglow emission, independent of knowledge of the ozone abundance, the ozone absorption cross sections, and the ultraviolet solar irradiance in the ozone Hartley band. Fifty-six months of airglow data taken between 1982 and 1986 by the near-infrared spectrometer on the Solar-Mesosphere Explorer satellite are analyzed. The energy deposition rates exhibit altitude-dependent annual and semi-annual variations. We also find a positive correlation between temperatures and energy deposition rates near 90 km at low latitudes. This correlation is largely due to the semiannual oscillation in temperature and ozone and is consistent with model calculations. There is also a suggestion of possible tidal enhancement of this correlation based on recent theoretical and observational analyses. The airglow-derived rates of energy deposition are then compared with those computed by multidimensional numerical models. The observed and modeled deposition rates typically agree to within 20%. This agreement in energy deposition rates implies the same agreement exists between measured and modeled ozone volume mixing ratios in the mesosphere. Only in the upper mesosphere at midlatitudes during winter do we derive energy deposition rates (and hence ozone mixing ratios) consistently and significantly larger than the model calculations. This result is contrary to previous studies that have shown a large model deficit in the ozone abundance throughout the mesosphere. The climatology of solar energy deposition and heating presented in this paper is available to the community at the Middle Atmosphere Energy Budget Project web site at http://heat-budget.gats-inc.com.

  19. Validation of Empirically Derived Rating Scales for a Story Retelling Speaking Test

    ERIC Educational Resources Information Center

    Hirai, Akiyo; Koizumi, Rie

    2013-01-01

    In recognition of the rating scale as a crucial tool of performance assessment, this study aims to establish a rating scale suitable for a Story Retelling Speaking Test (SRST), which is a semidirect test of speaking ability in English as a foreign language for classroom use. To identify an appropriate scale, three rating scales, all of which have…

  20. Summary of information on low-speed lateral-directional derivatives due to rate of change of sideslip beta prime

    NASA Technical Reports Server (NTRS)

    Coe, P. L., Jr.; Graham, A. B.; Chambers, J. R.

    1975-01-01

    The results presented show that the magnitudes of the aerodynamic stability derivatives due to rate of change of sideslip become quite large at high angles of attack for swept- and delta-wing configurations, and that such derivatives have large effects on the calculated dynamic stability of these configurations at high angles of attack. The wind-tunnel test techniques used to measure the beta prime derivatives and various approaches used to predict them are discussed. Both the conventional oscillating-airfoil theory and the lag-of-the-sidewash theory are shown to be inadequate for predicting the vertical-tail contribution to the acceleration-in-sideslip derivative; a flow-field-lag theory, which is discussed, appears to give qualitative agreement with experimental data for a current twin-jet fighter configuration.

  1. Late Quaternary sediment-accumulation rates within the inner basins of the California Continental Borderland in support of geologic hazard evaluation

    USGS Publications Warehouse

    Normark, W.R.; McGann, M.; Sliter, R.W.

    2009-01-01

    An evaluation of the geologic hazards of the inner California Borderland requires determination of the timing for faulting and mass-movement episodes during the Holocene. Our effort focused on basin slopes and turbidite systems on the basin floors for the area between Santa Barbara and San Diego, California. Dating condensed sections on slopes adjacent to fault zones provides better control on fault history where high-resolution, seismic-reflection data can be used to correlate sediment between the core site and the fault zones. This study reports and interprets 147 radiocarbon dates from 43 U.S. Geological Survey piston cores as well as 11 dates from Ocean Drilling Program Site 1015 on the floor of Santa Monica Basin. One hundred nineteen dates from 39 of the piston cores have not previously been published. Core locations were selected for hazard evaluation, but despite the nonuniform distribution of sample locations, the dates obtained for the late Quaternary deposits are useful for documenting changes in sediment-accumulation rates during the past 30 ka. Cores from basins receiving substantial sediment from rivers, i.e., Santa Monica Basin and the Gulf of Santa Catalina, show a decrease in sediment supply during the middle Holocene, but during the late Holocene after sea level had reached the current highstand condition, rates then increased partly in response to an increase in El Ni??o-Southern Oscillation events during the past 3.5 ka. ?? 2009 The Geological Society of America.

  2. Growth Kinetics and Morphology of Barite Crystals Derived from Face-Specific Growth Rates

    SciTech Connect

    Godinho, Jose R. A.; Stack, Andrew G.

    2015-03-30

    Here we investigate the growth kinetics and morphology of barite (BaSO4) crystals by measuring the growth rates of the (001), (210), (010), and (100) surfaces using vertical scanning interferometry. Solutions with saturation indices 1.1, 2.1, and 3.0 without additional electrolyte, in 0.7 M NaCl, or in 1.3 mM SrCl2 are investigated. Face-specific growth rates are inhibited in the SrCl2 solution relative to a solution without electrolyte, except for (100). Contrarily, growth of all faces is promoted in the NaCl solution. The variation of face-specific rates is solution-specific, which leads to a. change of the crystal morphology and overall growth rate of crystals. The measured face-specific growth rates are used to model the growth of single crystals. Modeled crystals have a morphology and size similar to those grown from solution. Based on the model the time dependence of surface area and growth rates is analyzed. Growth rates change with time due to surface area normalization for small crystals and large growth intervals. By extrapolating rates to crystals with large surfaces areas, time-independent growth rates are 0.783, 2.96, and 0.513 mmol∙m-2∙h-1, for saturation index 2.1 solutions without additional electrolyte, NaCl, and SrCl2, respectively.

  3. Growth Kinetics and Morphology of Barite Crystals Derived from Face-Specific Growth Rates

    DOE PAGES

    Godinho, Jose R. A.; Stack, Andrew G.

    2015-03-30

    Here we investigate the growth kinetics and morphology of barite (BaSO4) crystals by measuring the growth rates of the (001), (210), (010), and (100) surfaces using vertical scanning interferometry. Solutions with saturation indices 1.1, 2.1, and 3.0 without additional electrolyte, in 0.7 M NaCl, or in 1.3 mM SrCl2 are investigated. Face-specific growth rates are inhibited in the SrCl2 solution relative to a solution without electrolyte, except for (100). Contrarily, growth of all faces is promoted in the NaCl solution. The variation of face-specific rates is solution-specific, which leads to a. change of the crystal morphology and overall growth ratemore » of crystals. The measured face-specific growth rates are used to model the growth of single crystals. Modeled crystals have a morphology and size similar to those grown from solution. Based on the model the time dependence of surface area and growth rates is analyzed. Growth rates change with time due to surface area normalization for small crystals and large growth intervals. By extrapolating rates to crystals with large surfaces areas, time-independent growth rates are 0.783, 2.96, and 0.513 mmol∙m-2∙h-1, for saturation index 2.1 solutions without additional electrolyte, NaCl, and SrCl2, respectively.« less

  4. Experiments probing the influence of air exchange rates on secondary organic aerosols derived from indoor chemistry

    NASA Astrophysics Data System (ADS)

    Weschler, Charles J.; Shields, Helen C.

    Reactions between ozone and terpenes have been shown to increase the concentrations of submicron particles in indoor settings. The present study was designed to examine the influence of air exchange rates on the concentrations of these secondary organic aerosols as well as on the evolution of their particle size distributions. The experiments were performed in a manipulated office setting containing a constant source of d-limonene and an ozone generator that was remotely turned "on" or "off" at 6 h intervals. The particle number concentrations were monitored using an optical particle counter with eight-channels ranging from 0.1-0.2 to>2.0 μm diameter. The air exchange rates during the experiments were either high (working hours) or low (non-working hours) and ranged from 1.6 to>12 h -1, with intermediate exchange rates. Given the emission rates of ozone and d-limonene used in these studies, at an air exchange rate of 1.6 h -1 particle number concentration in the 0.1-0.2 μm size-range peaked 1.2 h after the ozone generator was switched on. In the ensuing 4.8 h particle counts increased in successive size-ranges up to the 0.5-0.7 μm diameter range. At higher air exchange rates, the resulting concentrations of total particles and particle mass (calculated from particle counts) were smaller, and at exchange rates exceeding 12 h -1, no excess particle formation was detectable with the instrument used in this study. Particle size evolved through accretion and, in some cases, coagulation. There was evidence for coagulation among particles in the smallest size-range at low air exchange rates (high particle concentrations) but no evidence of coagulation was apparent at higher air exchange rates (lower particle concentrations). At higher air exchange rates the particle count or size distributions were shifted towards smaller particle diameters and less time was required to achieve the maximum concentration in each of the size-ranges where discernable particle growth

  5. Deriving time-dependent diffusion and relaxation rate in porous systems using eigenfunctions of the Laplace operator.

    PubMed

    Nordin, Matias; Jacobi, Martin Nilsson; Nydén, Magnus

    2009-12-01

    Porous systems are investigated using eigendecomposition of the Laplace matrix. Three parameters; tortuosity, surface-to-pore volume ratio and relaxation rate are derived from the eigenvalue spectrum of the Laplace matrix and connected to the parameters in the Padé approximation, an expression often used to describe the time-dependent diffusion coefficient in porous systems. The Padé length is identified for systems with large pore to connector volume ratio. The results are compared with simulations.

  6. Carbon accumulation in a permafrost polygon peatland: steady long-term rates in spite of shifts between dry and wet conditions.

    PubMed

    Gao, Yang; Couwenberg, John

    2015-02-01

    Ice-wedge polygon peatlands contain a substantial part of the carbon stored in permafrost soils. However, little is known about their long-term carbon accumulation rates (CAR) in relation to shifts in vegetation and climate. We collected four peat profiles from one single polygon in NE Yakutia and cut them into contiguous 0.5 cm slices. Pollen density interpolation between AMS (14)C dated levels provided the time span contained in each of the sample slices, which--in combination with the volumetric carbon content--allowed for the reconstruction of CAR over decadal and centennial timescales. Vegetation representing dry palaeo-ridges and wet depressions was reconstructed with detailed micro- and macrofossil analysis. We found repeated shifts between wet and dry conditions during the past millennium. Dry ridges with associated permafrost growth originated during phases of (relatively) warm summer temperature and collapsed during relatively cold phases, illustrating the important role of vegetation and peat as intermediaries between ambient air temperature and the permafrost. The average long-term CAR across the four profiles was 10.6 ± 5.5 g C m(-2) yr(-1). Time-weighted mean CAR did not differ significantly between wet depression and dry ridge/hummock phases (10.6 ± 5.2 g C m(-2) yr(-1) and 10.3 ± 5.7 g C m(-2) yr(-1), respectively). Although we observed increased CAR in relation to warm shifts, we also found changes in the opposite direction and the highest CAR actually occurred during the Little Ice Age. In fact, CAR rather seems to be governed by strong internal feedback mechanisms and has roughly remained stable on centennial time scales. The absence of significant differences in CAR between dry ridge and wet depression phases suggests that recent warming and associated expansion of shrubs will not affect long-term rates of carbon burial in ice-wedge polygon peatlands.

  7. Carbon accumulation in a permafrost polygon peatland: steady long-term rates in spite of shifts between dry and wet conditions.

    PubMed

    Gao, Yang; Couwenberg, John

    2015-02-01

    Ice-wedge polygon peatlands contain a substantial part of the carbon stored in permafrost soils. However, little is known about their long-term carbon accumulation rates (CAR) in relation to shifts in vegetation and climate. We collected four peat profiles from one single polygon in NE Yakutia and cut them into contiguous 0.5 cm slices. Pollen density interpolation between AMS (14)C dated levels provided the time span contained in each of the sample slices, which--in combination with the volumetric carbon content--allowed for the reconstruction of CAR over decadal and centennial timescales. Vegetation representing dry palaeo-ridges and wet depressions was reconstructed with detailed micro- and macrofossil analysis. We found repeated shifts between wet and dry conditions during the past millennium. Dry ridges with associated permafrost growth originated during phases of (relatively) warm summer temperature and collapsed during relatively cold phases, illustrating the important role of vegetation and peat as intermediaries between ambient air temperature and the permafrost. The average long-term CAR across the four profiles was 10.6 ± 5.5 g C m(-2) yr(-1). Time-weighted mean CAR did not differ significantly between wet depression and dry ridge/hummock phases (10.6 ± 5.2 g C m(-2) yr(-1) and 10.3 ± 5.7 g C m(-2) yr(-1), respectively). Although we observed increased CAR in relation to warm shifts, we also found changes in the opposite direction and the highest CAR actually occurred during the Little Ice Age. In fact, CAR rather seems to be governed by strong internal feedback mechanisms and has roughly remained stable on centennial time scales. The absence of significant differences in CAR between dry ridge and wet depression phases suggests that recent warming and associated expansion of shrubs will not affect long-term rates of carbon burial in ice-wedge polygon peatlands. PMID:25230297

  8. Loudness function derives from data on electrical discharge rates in auditory nerve fibers

    NASA Technical Reports Server (NTRS)

    Howes, W. L.

    1973-01-01

    Judgements of the loudness of pure-tone sound stimuli yield a loudness function which relates perceived loudness to stimulus amplitude. A loudness function is derived from physical evidence alone without regard to human judgments. The resultant loudness function is L=K(q-q0), where L is loudness, q is effective sound pressure (specifically q0 at the loudness threshold), and K is generally a weak function of the number of stimulated auditory nerve fibers. The predicted function is in agreement with loudness judgment data reported by Warren, which imply that, in the suprathreshold loudness regime, decreasing the sound-pressure level by 6 db results in halving the loudness.

  9. Vertical displacement rates in the Upper Rhine Graben area derived from precise leveling

    NASA Astrophysics Data System (ADS)

    Fuhrmann, Thomas; Westerhaus, Malte; Zippelt, Karl; Heck, Bernhard

    2014-08-01

    The recent vertical displacement field of the Upper Rhine Graben (URG) located in the tri-national region between Germany, France and Switzerland is investigated using repeatedly measured leveling data. We estimate vertical displacement rates at leveling benchmarks by applying a kinematic network adjustment on more than 40,000 height differences measured by German, French and Swiss surveying agencies. Focusing on an optimal solution for the adjusted rates in the URG area also historical data (measured before 1900) are used, significantly increasing the time span of available measurements and the number of transnational connections between the three countries. To account for inhomogeneities apparent in the database, we apply an iterative variance component estimation within the adjustment procedure, particularly revealing more realistic information on the accuracy of the estimated rates. A special focus within our analysis is put on the statistical testing of gross errors in the observations and model-related errors at benchmarks with non-linear movement. As some of the estimated vertical rates behave significantly different compared to the vertical rates of adjacent benchmarks, a filtering of outliers is applied after the adjustment procedure. The resulting map of linear height changes in an area of 280 km in N-S and 230 km in E-W direction provides detailed insight into the recent vertical displacements of the URG and neighboring regions. In the German part of the study area, it was possible for the first time to consistently constrain an average subsidence rate of 0.5 mm/a (0.2 mm/a) of the Graben interior w.r.t. the Black Forest. In addition to the tectonic displacements, some man-induced surface movements, e.g., caused by oil and groundwater extraction, are observed and discussed.

  10. Videopanorama Frame Rate Requirements Derived from Visual Discrimination of Deceleration During Simulated Aircraft Landing

    NASA Technical Reports Server (NTRS)

    Furnstenau, Norbert; Ellis, Stephen R.

    2015-01-01

    In order to determine the required visual frame rate (FR) for minimizing prediction errors with out-the-window video displays at remote/virtual airport towers, thirteen active air traffic controllers viewed high dynamic fidelity simulations of landing aircraft and decided whether aircraft would stop as if to be able to make a turnoff or whether a runway excursion would be expected. The viewing conditions and simulation dynamics replicated visual rates and environments of transport aircraft landing at small commercial airports. The required frame rate was estimated using Bayes inference on prediction errors by linear FRextrapolation of event probabilities conditional on predictions (stop, no-stop). Furthermore estimates were obtained from exponential model fits to the parametric and non-parametric perceptual discriminabilities d' and A (average area under ROC-curves) as dependent on FR. Decision errors are biased towards preference of overshoot and appear due to illusionary increase in speed at low frames rates. Both Bayes and A - extrapolations yield a framerate requirement of 35 < FRmin < 40 Hz. When comparing with published results [12] on shooter game scores the model based d'(FR)-extrapolation exhibits the best agreement and indicates even higher FRmin > 40 Hz for minimizing decision errors. Definitive recommendations require further experiments with FR > 30 Hz.

  11. 77 FR 5416 - Financial Derivatives Transactions To Offset Interest Rate Risk; Investment and Deposit Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-03

    ... concerned about the impact of future rising interest rates on credit unions' balance sheets, especially... depending on the nature and complexity of an FCU's balance sheet. As noted in ANPR I, the Board is... a less complex balance sheet, is it sufficient for that FCU's staff to demonstrate a minimum...

  12. Trends in rain rate distribution derived from gauges in Hong Kong

    NASA Astrophysics Data System (ADS)

    Lau, Stephen Pw; Chiu, Long S.; Lee, T. C.

    2010-05-01

    The impact of the increase in emission of greenhouse gases and changes in land cover/land use pattern have resulted in change in the general circulation of the atmosphere and the energy and water cycles. While there is general concensus in trends in temperature, the changes in the water cycle, in particular precipitation is still uncertain. Climate models predict relatively small change in the total rainfall, however, the distribution of rain rates becomes more extreme, i.e. increases in heavy and light or no/rain and decrease in the moderate rain categories. These complicated issues are compounded by the increase aerosols resulted from pollution, particularly in Megacities such as Hong Kong. To assess the changes associated with the regional hydrologic cycle due to these factors, we examine a long time series (starting in the late 1940s) of hourly rainfall of all gauge stations in Hong Kong maintained by the Hong Kong Observatory. In this presentation, annual variations of the rain rate distribuitons, the diurnal cycle, and rain event characteristics (duration, storm total, conditional rain rates, and separation) for the winter monsoon, summer monsoon, mid-summer rain and typhoon periods will be presented. Trends of the rain rate categories, event statistics, diurnal variabiltiy, will be examined by linear regression analysis, Empirical Mode Decomposition and wavelet analysis.

  13. Erosion rates along fault scarps and rift-shoulder environments in central and northern Kenya: Insights from new 10Be-derived basin-wide erosion rates

    NASA Astrophysics Data System (ADS)

    Torres-Acosta, V.; Strecker, M. R.; Schildgen, T. F.; Wittmann, H.; Scherler, D.; Bookhagen, B.

    2011-12-01

    The Kenya Rift is typical example of an active continental rift zone and is a fundamental part of the East African Rift system. The rift valley plays a central role in archiving the relationships between sedimentation, erosion, and climate in the region. However, the links between surface processes (i.e., erosion, sedimentation) and tectonic setting are currently poorly understood. In this study we analyze to what degree tectono-geomorphic setting and/or climatic characteristics control erosion rates in the region. We extract morphometric characteristics of the rift flanks and the plateau surface from SRTM 90-m resolution digital elevation data. We rely on calibrated, satellite-derived Tropical Rainfall Measurement Mission (TRMM 2B31) rainfall to characterize the different climatic compartments throughout the study region. We calculate specific stream power amounts using integrated rainfall as discharge amounts. Next, we analyze the relation between cosmogenic radionuclide (CRN) basin-wide erosion rates and climatic and geomorphic parameters. We determined erosion rates from twenty-six river sand samples acquired from along the flanks of the Elgeyo Escarpment (northern section of western rift flank), the Nguruman Escarpment (southern section of western flank), the Tirr Tirr Plateau (north), the Kapute Plains, and the Suguta Valley. Catchment-wide erosion rates range from 0.001 to 0.1 mm/y across the different climatic compartments. Comparisons to catchment climate and topographic characteristics suggest that more than 60% of variation in erosion can be explain by specific stream power amounts using rainfall as discharge component. The catchment-averaged normalized channel steepness index, which doesn't take into account variations in precipitation, explains only 42% of the variation in erosion rates. These observations demonstrate that the strong spatial variations in erosion rates are largely controlled by both catchment morphology and climatic gradients. In

  14. Martian impact cratering rate over the last 3 billions years derived from layered ejecta craters dating

    NASA Astrophysics Data System (ADS)

    Lagain, Anthony; Bouley, Sylvain; Costard, François; Baratoux, David

    2016-04-01

    All chronology models used in dating planetary surfaces are based on the lunar chronology system. The cratering density of the Moon has been calibrated with absolute ages from Apollo lunar samples. However, there are no lunar samples between 3 Gy and 800 My and only four samples have been dated between 800 My and present. Therefore, the evolution of the cratering rate after the LHB and before 3 Gy is well constrained. The cratering rate between 3 Gy and present has been assumed to be constant [1, 2]. Nevertheless, this assumption is challenged by the analysis of the geological record, such as the frequency of landslide on Mars as a function of time [3, 4]. It is therefore necessary to re-examine the validity of this assumption and place constraints on the cratering rate since the last 3 Gy. For this purpose, we study the rate of impact cratering using small craters on a set of 53 layered ejecta craters larger than 5 km in diameter in Acidalia Planitia, Mars. LECs larger than 5km have large enough surfaces to date their formation by counting craters larger than 100m present on their blankets. Furthermore, limits of their ejecta blankets are clearly defined by a terminal bead. In order to determine the crater emplacement ages, we have applied the methodology dating described in our previous study [6] on all ejecta layers. Errors on measured ages were calculated following [7]. The age of the study area is 2.8±0.2 Gy. Our crater counts on distal ejecta blankets reveal ages younger than the age of the surrounding surface, as expected. It is essential to take into account errors on measured ages. The statistical sample used to build this emplacement frequency distribution and our dating methodology are sufficiently reliable to deduce that a constant impact cratering rate over the last 3 Gy is not a correct approximation. The excessive number of craters emplaced 1Gy ago compared to the cratering rate used suggests a decreased impact cratering rate over the last 1Gy and

  15. Disparities between in situ and optically derived carbon biomass and growth rates of the prymnesiophyte Phaeocystis globosa

    NASA Astrophysics Data System (ADS)

    Peperzak, L.; van der Woerd, H. J.; Timmermans, K. R.

    2015-03-01

    The oceans play a pivotal role in the global carbon cycle. It is not practical to measure the global daily production of organic carbon, the product of phytoplankton standing stock and its growth rate using discrete oceanographic methods. Instead, optical proxies from Earth-orbiting satellites must be used. To test the accuracy of optically derived proxies of phytoplankton physiology and growth rate, hyperspectral reflectance data from the wax and wane of a Phaeocystis bloom in laboratory mesocosms were compared with standard ex situ data. Chlorophyll biomass could be estimated accurately from reflectance using specific chlorophyll absorption algorithms. However, the conversion of chlorophyll (Chl) to carbon (C) was obscured by the non-linear increase in C : Chl under nutrient-limited growth. Although C : Chl was inversely correlated (r2 = 0.88) with the in situ fluorometric growth rate indicator Fv / Fm (Photosystem II quantum efficiency), none of them was linearly correlated to growth rate, constraining the accurate calculation of Phaeocystis growth or production rates. Unfortunately, the optical proxy ϕph (quantum efficiency of fluorescence: the ratio of the number of fluoresced photons to the number of photons absorbed by the phytoplankton) did not show any correlation with Phaeocystis growth rate, and therefore it is concluded that ϕph cannot be applied in the remotely sensed measurement of this species' carbon production rate.

  16. Simultaneous determination of 222Rn and 220Rn exhalation rates from building materials used in Central Italy with accumulation chambers and a continuous solid state alpha detector: influence of particle size, humidity and precursors concentration.

    PubMed

    Tuccimei, P; Moroni, M; Norcia, D

    2006-02-01

    A method to determine simultaneously the rates of 222Rn and 220Rn released from building materials quarried in Central Italy is presented. The method makes use of a continuous monitor equipped with a solid state alpha detector, in-line connected to a small accumulation chamber. The effects of chamber leakage and back diffusion on 222Rn free exhalation rate is evaluated. The influence of available exhalation surface, humidity content and precursors concentration on radon and thoron exhalation rates is investigated.

  17. Denudation rates derived from spatially-averaged cosmogenic nuclide analysis in Nelson catchments, South Island, New Zealand

    NASA Astrophysics Data System (ADS)

    Burdis, A.; Norton, K. P.; Ditchburn, B.; Zondervan, A.

    2013-12-01

    New Zealand's tectonically and climatically dynamic environment generates erosion rates that outstrip global averages by up to ten times in some locations. In order to assess recent changes in erosion rate, and also to predict future erosion dynamics, it is important to quantify long-term, background erosion. Current research on erosion in New Zealand predominantly covers short-term (100 yrs) erosion dynamics and Myr dynamics from thermochronological proxy data. Without medium-term denudation data for New Zealand, it is uncertain which variables (climate, anthropogenic disturbance of the landscape, tectonic uplift, lithological, or geomorphic characteristics) exert the dominant control on denudation in New Zealand. Spatially-averaged cosmogenic nuclide analysis can effectively offer this information by providing averaged rates of denudation on millennial timescales without the biases and limitations of short-term erosion methods. Basin-averaged denudation rates were obtained in the Nelson region, New Zealand, from analysis of concentrations of meteoric 10Be in clay and in-situ produced 10Be in quartz. The measured denudation rates integrate over ~8000 yrs (meteoric) and ~3000 yrs (in-situ). Not only do the 10Be records produce erosion rates that are remarkably consistent with each other, but they are also independent of topographic metrics. Denudation rates range from ~116 - 306 t km-2 yr-1, with the exception of one basin which is eroding at 789 t km-2 yr-1(derived from meteoric 10Be) and 644 t km-2 yr-1(derived from in-situ 10Be). The homogeneity of rates and absence of a significant correlation with geomorphic or lithological characteristics suggest another factor is exerting the dominant control on landscape denudation in the Nelson region. Storm variability is a likely driver of erosion in this setting. The background rates are higher than current short term rates (~50 - 200 t km-2 yr-1) due to the significant erosion caused by high magnitude, low frequency

  18. Synoptic-scale variability of satellite-derived sea-ice deformation rates in the Arctic

    NASA Astrophysics Data System (ADS)

    Herman, A.; Głowacki, O.

    2012-04-01

    Observational data show that deformation of the compact sea ice covering the central Arctic takes place within elongated, narrow zones separating semi-rigid floes. Localization of deformation, and a related intermittent character of internal stress in the ice, cannot be satisfactorily reproduced with present state-of-the-art numerical models, especially those based on various versions of viscous-plastic rheology. Similarly, sea-ice models do not reproduce properly the observed power-law tails of deformation-rate probability distributions (pdfs), with a slope depending on the scale of the observation. In order to be able to improve the models, one needs: (i) relevant quantitative measures of ice deformation rates that the models should aim to reproduce; (ii) a better understanding of the time variability of those measures (existing studies are usually limited to the analysis of single events) and their dependence on changes of the external forcing and of the properties of the ice itself. In this study, we use gridded sea-ice total deformation rates from the RGPS data provided by the RADARSAT-1 satellite, available for 11 winter seasons with a time resolution of 3 days and a spatial resolution of 12.55 km. The analysis is based on deformation-rate pdfs obtained by means of a rank-order analysis of the data for each snapshot in the dataset. We analyze the time variability of: (i) the slope of the power-law tails of the pdfs, estimated with a maximum-likelihood method; and (ii) the moments of the pdfs for a range of exponents q and spatial scales L from the original mesh size to approximately 1000 km. In all analyzed cases, the slope of the moments as a function of the length scale L increases (faster than linearly) with increasing power q. However, the tempo of this increase can be very different. Generally, there are two distinct, dominating patterns of variability, with the first pattern describing the overall level of deformation, and the second one being generally

  19. Escape rate of Brownian particles from a metastable potential well under time derivative Ornstein-Uhlenbeck noise

    NASA Astrophysics Data System (ADS)

    Bai, Zhan-Wu; Wang, Ping

    2016-03-01

    We investigate the escape rate of Brownian particles that move in a cubic metastable potential subjected to an internal time derivative Ornstein-Uhlenbeck noise (DOUN). This noise can induce the ballistic diffusion of force-free Brownian particles. Some new features are found. The escape rate for DOUN shows qualitative different dependence on potential well width compared with OUN which induces normal diffusion. As the potential barrier height decreases, the escape rate of DOUN deviates from Arrhenius law considerably earlier than that of Ornstein-Uhlenbeck noise (OUN). The Brownian particles escape faster under DOUN than that under OUN. A quasi-periodic oscillation occurs in transient state. A solvable case is presented to demonstrate the significant cancellation behavior in the barrier region that governs most of these phenomena. The physical mechanism of the findings can be clarified by the noise features. These characteristics should be common for internal noises that induce superdiffusion, especially the ballistic diffusion.

  20. Asynchronous RTK precise DGNSS positioning method for deriving a low-latency high-rate output

    NASA Astrophysics Data System (ADS)

    Liang, Zhang; Hanfeng, Lv; Dingjie, Wang; Yanqing, Hou; Jie, Wu

    2015-07-01

    Low-latency high-rate (1 Hz) precise real-time kinematic (RTK) can be applied in high-speed scenarios such as aircraft automatic landing, precise agriculture and intelligent vehicle. The classic synchronous RTK (SRTK) precise differential GNSS (DGNSS) positioning technology, however, is not able to obtain a low-latency high-rate output for the rover receiver because of long data link transmission time delays (DLTTD) from the reference receiver. To overcome the long DLTTD, this paper proposes an asynchronous real-time kinematic (ARTK) method using asynchronous observations from two receivers. The asynchronous observation model (AOM) is developed based on undifferenced carrier phase observation equations of the two receivers at different epochs with short baseline. The ephemeris error and atmosphere delay are the possible main error sources on positioning accuracy in this model, and they are analyzed theoretically. In a short DLTTD and during a period of quiet ionosphere activity, the main error sources decreasing positioning accuracy are satellite orbital errors: the "inverted ephemeris error" and the integration of satellite velocity error which increase linearly along with DLTTD. The cycle slip of asynchronous double-differencing carrier phase is detected by TurboEdit method and repaired by the additional ambiguity parameter method. The AOM can deal with synchronous observation model (SOM) and achieve precise positioning solution with synchronous observations as well, since the SOM is only a specific case of AOM. The proposed method not only can reduce the cost of data collection and transmission, but can also support the mobile phone network data link transfer mode for the data of the reference receiver. This method can avoid data synchronizing process besides ambiguity initialization step, which is very convenient for real-time navigation of vehicles. The static and kinematic experiment results show that this method achieves 20 Hz or even higher rate output in

  1. A record of barite accumulation rate for marine export productivity changes in the tropical Indian Ocean during the Mid-Pliocene--Early-Pleistocene transition

    NASA Astrophysics Data System (ADS)

    Zhou, Liping; Ma, Zhongwu; Ding, Xuan

    2016-04-01

    One of the most interesting features in the marine oxygen isotope records is the gradual shift towards heavier 18O from the Mid-Pliocene, which ends with the initiation of Northern Hemisphere glaciation (NHG) around 2.7 Ma. The lack of significant change in sea surface temperature in the tropical Indian Ocean as revealed in the previous studies does not rule out their possible contributions to this dramatic climate change during the Mid-Pliocene transition. Changing circulation systems in the region will control the supply of nutrients for the water masses which in turn determine the marine productivity. In the areas of high productivity, ocean export productivity may potentially provide a mechanism of CO2 draw-down into the deep ocean, through which contributing to the lowering of the global temperature. In this study, we present a record of barite accumulation rate (BAR) for DSDP Site 214 drilled on the Ninetyeast Ridge. Here we use the marine barite, which is formed during the decay of organism in the twilight zone, as a proxy for ocean export productivity. Our results show that the BAR of Site 214 varies between 0.25 and 1.25 mg/cm2/kyr during the period between 4 Ma and 2 Ma. Five intervals of increased BAR from 3.6 Ma to 2.4 Ma are identified with the most distinct peak centred around 3 Ma. The overall pattern does not follow either the oxygen isotope record for the Site or the sea surface temperature and subsurface temperature reconstructed with the Mg/Ca of foraminifera. This suggests that regional changes in ocean circulation and water masses may have played more important role than temperature in controlling the productivity change in the tropical Indian Ocean. The relative higher productivity around 3 Ma may imply a biogenetic process towards the intensification of NHGs.

  2. High-rate electrochemical capacitors based on ordered mesoporous silicon carbide-derived carbon.

    PubMed

    Korenblit, Yair; Rose, Marcus; Kockrick, Emanuel; Borchardt, Lars; Kvit, Alexander; Kaskel, Stefan; Yushin, Gleb

    2010-03-23

    Microporous carbons, produced by selective etching of metal carbides in a chlorine-containing environment, offer narrow distribution of micropores and one of the highest specific capacitances reported when used in electrical double layer capacitors (EDLC) with organic electrolytes. Previously, the small micropores in these carbons served as an impediment to ion transport and limited the power storage characteristics of EDLC. Here we demonstrate, for the first time, how the preparation and application of templated carbide-derived carbon (CDC) can overcome the present limitations and show the route for dramatic performance enhancement. The ordered mesoporous channels in the produced CDC serve as ion-highways and allow for very fast ionic transport into the bulk of the CDC particles. The enhanced transport led to 85% capacitance retention at current densities up to approximately 20 A/g. The ordered mesopores in silicon carbide precursor also allow the produced CDC to exhibit a specific surface area up to 2430 m(2)/g and a specific capacitance up to 170 F/g when tested in 1 M tetraethylammonium tetrafluoroborate solution in acetonitrile, nearly doubling the previously reported values. PMID:20180559

  3. Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol.

    PubMed

    Polburee, Pirapan; Yongmanitchai, Wichien; Lertwattanasakul, Noppon; Ohashi, Takao; Fujiyama, Kazuhito; Limtong, Savitree

    2015-12-01

    This study attempted to identify oleaginous yeasts and selected the strain that accumulated the largest quantity of lipid for lipid production from glycerol. Two-step screening of 387 yeast strains revealed 23 oleaginous strains that accumulated quantities of lipid higher than 20 % of their biomass when cultivated in glycerol. These strains were identified to be four ascomycetous yeast species i.e. Candida silvae, Kodamaea ohmeri, Meyerozyma caribbica, and Pichia manshurica, and five basidiomycetous yeast species i.e. Cryptococcus cf. podzolicus, Cryptococcus laurentii, Rhodosporidium fluviale, Rhodotorula taiwanensis, and Sporidiobolus ruineniae. Rhodosporidium fluviale DMKU-RK253 accumulated the highest quantity of lipid equal to 65.2 % of its biomass (3.9 g L(-1) lipid and 6.0 g L(-1) biomass) by shaking flask cultivation in crude glycerol. The main fatty acids in the accumulated lipid of this strain consisted of oleic acid, linoleic acid, and palmitic acid. Therefore, R. fluviale DMKU-RK253 has potential for producing lipid for biodiesel manufacturing using crude glycerol as a feedstock.

  4. Automatic stress-relieving music recommendation system based on photoplethysmography-derived heart rate variability analysis.

    PubMed

    Shin, Il-Hyung; Cha, Jaepyeong; Cheon, Gyeong Woo; Lee, Choonghee; Lee, Seung Yup; Yoon, Hyung-Jin; Kim, Hee Chan

    2014-01-01

    This paper presents an automatic stress-relieving music recommendation system (ASMRS) for individual music listeners. The ASMRS uses a portable, wireless photoplethysmography module with a finger-type sensor, and a program that translates heartbeat signals from the sensor to the stress index. The sympathovagal balance index (SVI) was calculated from heart rate variability to assess the user's stress levels while listening to music. Twenty-two healthy volunteers participated in the experiment. The results have shown that the participants' SVI values are highly correlated with their prespecified music preferences. The sensitivity and specificity of the favorable music classification also improved as the number of music repetitions increased to 20 times. Based on the SVI values, the system automatically recommends favorable music lists to relieve stress for individuals. PMID:25571461

  5. Automatic stress-relieving music recommendation system based on photoplethysmography-derived heart rate variability analysis.

    PubMed

    Shin, Il-Hyung; Cha, Jaepyeong; Cheon, Gyeong Woo; Lee, Choonghee; Lee, Seung Yup; Yoon, Hyung-Jin; Kim, Hee Chan

    2014-01-01

    This paper presents an automatic stress-relieving music recommendation system (ASMRS) for individual music listeners. The ASMRS uses a portable, wireless photoplethysmography module with a finger-type sensor, and a program that translates heartbeat signals from the sensor to the stress index. The sympathovagal balance index (SVI) was calculated from heart rate variability to assess the user's stress levels while listening to music. Twenty-two healthy volunteers participated in the experiment. The results have shown that the participants' SVI values are highly correlated with their prespecified music preferences. The sensitivity and specificity of the favorable music classification also improved as the number of music repetitions increased to 20 times. Based on the SVI values, the system automatically recommends favorable music lists to relieve stress for individuals.

  6. On rates and mechanisms of OH and O3 reactions with isoprene-derived hydroxy nitrates.

    PubMed

    Lee, Lance; Teng, Alex P; Wennberg, Paul O; Crounse, John D; Cohen, Ronald C

    2014-03-01

    Eight distinct hydroxy nitrates are stable products of the first step in the atmospheric oxidation of isoprene by OH. The subsequent chemical fate of these molecules affects global and regional production of ozone and aerosol as well as the location of nitrogen deposition. We synthesized and purified 3 of the 8 isoprene hydroxy nitrate isomers: (E/Z)-2-methyl-4-nitrooxybut-2-ene-1-ol and 3-methyl-2-nitrooxybut-3-ene-1-ol. Oxidation of these molecules by OH and ozone was studied using both chemical ionization mass spectrometry and thermo-dissociation laser induced fluorescence. The OH reaction rate constants at 300 K measured relative to propene at 745 Torr are (1.1 ± 0.2) × 10(-10) cm(3) molecule(-1) s(-1) for both the E and Z isomers and (4.2 ± 0.7) × 10(-11) cm(3) molecule(-1) s(-1) for the third isomer. The ozone reaction rate constants for (E/Z)-2-methyl-4-nitrooxybut-2-ene-1-ol are (2.7 ± 0.5) × 10(-17) and (2.9 ± 0.5) × 10(-17) cm(3) molecule(-1) s(-1), respectively. 3-Methyl-2-nitrooxybut-3-ene-1-ol reacts with ozone very slowly, within the range of (2.5-5) × 10(-19) cm(3) molecule(-1) s(-1). Reaction pathways, product yields, and implications for atmospheric chemistry are discussed. A condensed mechanism suitable for use in atmospheric chemistry models is presented.

  7. The new conversion model MODERN to derive erosion rates from inventories of fallout radionuclides

    NASA Astrophysics Data System (ADS)

    Arata, Laura; Meusburger, Katrin; Frenkel, Elena; A'Campo-Neuen, Annette; Iurian, Andra-Rada; Ketterer, Michael E.; Mabit, Lionel; Alewell, Christine

    2016-04-01

    The measurement of fallout radionuclides (FRNs) has become one of the most commonly used methods to quantify soil erosion and depositional processes. FRNs include anthropogenic radionuclides (e.g. 137Cs, 239+240Pu) released into the atmosphere during nuclear bomb tests and power plant accidents (e.g Chernobyl, Fukushima-Daiichi), as well as natural radiotracers such as 210Pbex and 7Be. FRNs reach the land surface by dry and wet fallouts from the atmosphere. Once deposited, FRNs are tightly adsorbed by fine soil particles and their subsequent redistribution is mostly associated with soil erosion processes. FRNs methods are based on a qualitative comparison: the inventory (total radionuclide activity per unit area) at a given sampling site is compared to that of a so called reference site. The conversion of FRN inventories into soil erosion and deposition rates is done with a variety of models, which suitability is dependent on the selected FRN, soil cultivation (ploughed or unploughed) and movement (erosion or deposition). The authors propose a new conversion model, which can be easily and comprehensively used for different FRNs, land uses and soil redistribution processes. This new model i.e. MODERN (MOdelling Deposition and Erosion rates with RadioNuclides) considers the precise depth distribution of a given FRN at a reference site, and allows adapting it for any specific site conditions. MODERN adaptability and performance has been tested on two published case studies: (i) a 137Cs study in an alpine and unploughed area in the Aosta valley (Italy) and (ii) a 210Pbex study on a ploughed area located in Romania. The results show a good agreement and a significant correlation (r= 0.91, p<0.0001) between the results of MODERN and the published models currently used by the FRN scientific community (i.e. the Profile Distribution Model and the Mass Balance Model). The open access code and the cost free accessibility of MODERN will ensure the promotion of a wider

  8. Effect of biofilm on fluorescence measurements derived from fast repetition rate fluorometers.

    PubMed

    Patil, Jagadish S; Saino, Toshiro

    2015-01-01

    This study evaluates, for the first time, the influence of biofilms on single and double optical window (SOW and DOW, respectively) fast repetition rate fluorometer (FRRF) measurements of microalgal photosystem-II initial fluorescence (F0), maximum fluorescence (Fm), variable fluorescence (Fv = Fm - F0), quantum yield (Fv/Fm) and functional absorption cross section (σPSII)]. Biofilms with chlorophyll > 0.1 μg cm(-2) and > 0.3 μgcm(-2) on SOW and DOW, respectively, produced a substantial increase in fluorescence. However, the relative magnitude of biofouling effects depended on sample chlorophyll concentrations, being more critical at concentrations < 1 mg m(-3). In DOW-FRRF, biofilms affected F0 (increased) and Fv/Fm (decreased) but not Fv and σPSII, whereas in SOW-FRRF, biofilms increased fluorescence and showed a variable effect on Fv/Fm and σPSII, because only biofilms on SOW attained actual Fm. As a result, the biofilm effect was substantial on SOW-FRRF measurements. On the other hand, the neutral-density filters (representing non-chlorophyll containing biofilms) with different transmission levels reduced the fluorescence signal. Correction procedures for the above photosystem-II parameters are proposed here.

  9. Unified expression for the rate constant of the bridged electron transfer derived by renormalization

    NASA Astrophysics Data System (ADS)

    Saito, Keisuke; Sumi, Hitoshi

    2009-10-01

    Electron transfer (ET) from a donor to an acceptor through an energetically close intermediary state on a midway molecule is a process found often in natural and artificial solar-energy capturing systems such as photosynthesis. This process has often been thought of in terms of opposing "superexchange" and "sequential or hopping" mechanisms, and the recent theory of Sumi and Kakitani (SK) [J. Phys. Chem. B 105, 9603 (2001)] has shown an interpolation between these mechanisms. In their theory, however, dynamics governing the most interesting intermediary region between them has artificially been introduced by phenomenologies. The dynamics is played by decoherence among electronic states, their decay, and thermalization of phonons in the medium. The present work clarifies the dynamics on a microscopic basis by means of renormalization in electronic coupling among the states, and gives a complete unified expression of the rate constant of the ET. It merges to that given by the SK theory in the semiclassical approximation for phonons interacting with an electron transferred.

  10. New Paleoclimate Records from the Russian Far East: Carbon Accumulation Rates and Ecological Change Over the Last 13,000 Years from Western and Central Kamchatka

    NASA Astrophysics Data System (ADS)

    Bochicchio, C. J.; Loisel, J.; Yu, Z.; Beilman, D.; Dirksen, V.; Dirksen, O.; Nichols, J. E.

    2014-12-01

    The Kamchatka peninsula lies along the confluence of the Pacific Ocean, and the Bering and Okhotsk Seas (OS). Its location is ideal to record shifts in regional ocean and atmospheric circulation and contains vast stores of carbon-rich peatlands, yet paleoclimate records from this area are scarce. This research aims to provide new paleo-proxy records that document carbon dynamics, precipitation, and temperature over the Holocene. We focus on site C4 (54.02 N, 156.13 E) located 18 km from OS coast at 91 m elevation and draw comparisons with site C1 (54.91 N, 156.60 E) which is 62 km from the coast at 256 m elevation, both in the Western Lowland (WL) region. Cores C1 and C4 are 450 and 375 cm in length, respectively. C4 and C1 were analyzed for organic matter (OM) content; while C4 received additional δ-deuterium (δD), plant macrofossil, and fossil pollen analysis. Both cores cover the last 13,000 years from the Western Lowlands (WL); prior to this study no such records existed from the WL or the eastern OS coastline. Chronologies are based on radiocarbon dating of fine fraction bulk peat and Sphagnum plant macrofossils. At both sites, peat accumulation began 11 ka (1 ka = 1000 calibrated years before present), is continuous to the surface, interlayered with tephra, and overlays a clay unit with 20% OM. OM density measured at 1 cm intervals show similar means of 0.1 g cm-3 over the last 11 ka and, despite the close proximity of the sites (103 km), they show two opposing OM trends: Period 1) From 5.8 to 3.5 ka, C1 OM density decreased ~0.10 to 0.06 g cm-3 while C4 increased from ~0.10 to 0.19 g cm-3, and Period 2) from 3.5 to 0.9 ka, C1 OM density increased to ~0.18 g cm-3 while C4 decreased to ~0.11 cm-3. Peat carbon accumulation rate (PCAR) is similar for both periods in C1 and C4 at 20.1 and 14.1 g m-2 yr-1, respectively. Prior to 5.8 ka PCAR in C1 and C4 is ~30 g m-2 yr-1 in the early Holocene, decreasing to 19 g m-2 yr-1 in the Mid- Late Holocene. C4 shows large

  11. QPO frequency derivative-frequency correlation indicates non-Keplerian boundary layer with a maximum in rotation rate

    NASA Astrophysics Data System (ADS)

    Alpar, M. Ali

    2016-10-01

    The correlation between the frequency and the absolute value of the frequency derivative of the kilohertz quasi-periodic oscillations (QPOs) observed for the first time from 4U 1636-53 is a simple consequence and indicator of the existence of a non-Keplerian rotation rate in the accretion disc boundary layer. This Letter interprets the observed correlation, showing that the observations provide strong evidence in support of the fundamental assumption of disc accretion models around slow rotators, that the boundary layer matches the Keplerian disc to the neutron star magnetosphere.

  12. Lipid thioesters derived from acylated proteins accumulate in infantile neuronal ceroid lipofuscinosis: correction of the defect in lymphoblasts by recombinant palmitoyl-protein thioesterase.

    PubMed Central

    Lu, J Y; Verkruyse, L A; Hofmann, S L

    1996-01-01

    Palmitoyl-protein thioesterase is a lysosomal long-chain fatty acyl hydrolase that removes fatty acyl groups from modified cysteine residues in proteins. Mutations in palmitoyl-protein thioesterase were recently found to cause the neurodegenerative disorder infantile neuronal ceroid lipofuscinosis, a disease characterized by accumulation of amorphous granular deposits in cortical neurons, leading to blindness, seizures, and brain death by the age of three. In the current study, we demonstrate that [35S]cysteine-labeled lipid thioesters accumulate in immortalized lymphoblasts of patients with infantile neuronal ceroid lipofuscinosis. The accumulation in cultured cells is reversed by the addition of recombinant palmitoyl-protein thioesterase that is competent for lysosomal uptake through the mannose-6-phosphate receptor. The [35S]cysteine-labeled lipids are substrates for palmitoyl-protein thioesterase in vitro, and their formation requires prior protein synthesis. These data support a role for palmitoyl-protein thioesterase in the lysosomal degradation of S-acylated proteins and define a major new pathway for the catabolism of acylated proteins in the lysosome. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 PMID:8816748

  13. Quantum reaction rate from higher derivatives of the thermal flux-flux autocorrelation function at time zero.

    PubMed

    Ceotto, Michele; Yang, Sandy; Miller, William H

    2005-01-22

    A quantum theory of thermal reaction rates is presented which may be viewed as an extension of the recently developed "quantum instanton" (QI) model [W. H. Miller, Y. Zhao, M. Ceotto, and S. Yang, J. Chem. Phys. 119, 1329 (2003)]. It is based on using higher derivatives of the flux-flux autocorrelation function C(t) (as given by Miller, Schwartz, and Tromp) at t=0 to construct a short time approximation for C(t). Tests of this theory on 1d and collinear reactions, both symmetric and asymmetric, show it to be more accurate than the original QI model, giving rate constants to approximately 5% for a wide range of temperature. PMID:15740237

  14. Quantum reaction rate from higher derivatives of the thermal flux-flux autocorrelation function at time zero

    NASA Astrophysics Data System (ADS)

    Ceotto, Michele; Yang, Sandy; Miller, William H.

    2005-01-01

    A quantum theory of thermal reaction rates is presented which may be viewed as an extension of the recently developed "quantum instanton" (QI) model [W. H. Miller, Y. Zhao, M. Ceotto, and S. Yang, J. Chem. Phys. 119, 1329 (2003)]. It is based on using higher derivatives of the flux-flux autocorrelation function C(t) (as given by Miller, Schwartz, and Tromp) at t=0 to construct a short time approximation for C(t). Tests of this theory on 1d and collinear reactions, both symmetric and asymmetric, show it to be more accurate than the original QI model, giving rate constants to ˜5% for a wide range of temperature.

  15. Partitioning modes and rates of sediment flux derived from terrace-channel coupling

    NASA Astrophysics Data System (ADS)

    Higson, J. L.; Singer, M. B.

    2012-12-01

    to harbor the Hg. We present results from an analysis of this model at one cross section with very high Hg contamination in the bounding terrace, where suitable hydrology, geometry, and grain size data were available. We modeled three separate years of observed hydrology on the Yuba, (1986, 1997 and 2006), each of which contains one of the largest floods of the last several decades. We assess under what conditions the terraces fail and identify their contribution to fine sediment/contaminant loads. We also provide a sensitivity analysis to initial terrace/channel GSD, porosity, permeability and antecedent moisture in the bank/terrace. We find that the largest first-order variable is antecedent moisture, particularly visible in the 1986 hydrograph containing two flood events. This double peak creates a lag in the response of ground water levels to the fluctuation of the river stage. Our coupled model allows for analysis of: the relative contribution of riverine sediment flux sourced from failed terraces; the existence of thresholds of failure; the importance of hydrograph shape; and the influence of bank/terrace failure on sediment flux rates and grain size distributions.

  16. Radar-derived asteroid shapes point to a 'zone of stability' for topography slopes and surface erosion rates

    NASA Astrophysics Data System (ADS)

    Richardson, J.; Graves, K.; Bowling, T.

    2014-07-01

    Previous studies of the combined effects of asteroid shape, spin, and self-gravity have focused primarily upon the failure limits for bodies with a variety of standard shapes, friction, and cohesion values [1,2,3]. In this study, we look in the opposite direction and utilize 22 asteroid shape-models derived from radar inversion [4] and 7 small body shape-models derived from spacecraft observations [5] to investigate the region in shape/spin space [1,2] wherein self-gravity and rotation combine to produce a stable minimum state with respect to surface potential differences, dynamic topography, slope magnitudes, and erosion rates. This erosional minimum state is self-correcting, such that changes in the body's rotation rate, either up or down, will increase slope magnitudes across the body, thereby driving up erosion rates non-linearly until the body has once again reached a stable, minimized surface state [5]. We investigated this phenomenon in a systematic fashion using a series of synthesized, increasingly prolate spheroid shape models. Adjusting the rotation rate of each synthetic shape to minimize surface potential differences, dynamic topography, and slope magnitudes results in the magenta curve of the figure (right side), defining the zone of maximum surface stability (MSS). This MSS zone is invariant both with respect to body size (gravitational potential and rotational potential scale together with radius), and density when the scaled-spin of [2] is used. Within our sample of observationally derived small-body shape models, slow rotators (Group A: blue points), that are not in the maximum surface stability (MSS) zone and where gravity dominates the slopes, will generally experience moderate erosion rates (left plot) and will tend to move up and to the right in shape/spin space as the body evolves (right plot). Fast rotators (Group C: red points), that are not in the MSS zone and where spin dominates the slopes, will generally experience high erosion rates

  17. Accumulate Repeat Accumulate Coded Modulation

    NASA Technical Reports Server (NTRS)

    Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

    2004-01-01

    In this paper we propose an innovative coded modulation scheme called 'Accumulate Repeat Accumulate Coded Modulation' (ARA coded modulation). This class of codes can be viewed as serial turbo-like codes, or as a subclass of Low Density Parity Check (LDPC) codes that are combined with high level modulation. Thus at the decoder belief propagation can be used for iterative decoding of ARA coded modulation on a graph, provided a demapper transforms the received in-phase and quadrature samples to reliability of the bits.

  18. Snow accumulation variability derived from radar and firn core data along a 600 km transect in Adelie Land, East Antarctic plateau

    NASA Astrophysics Data System (ADS)

    Verfaillie, D.; Fily, M.; Le Meur, E.; Magand, O.; Jourdain, B.; Arnaud, L.; Favier, V.

    2012-11-01

    The mass balance of ice sheets is an intensively studied topic in the context of global change and sea-level rise. However - particularly in Antarctica - obtaining mass balance estimates remains difficult due to various logistical problems. In the framework of the TASTE-IDEA (Trans-Antarctic Scientific Traverses Expeditions - Ice Divide of East Antarctica) program, an International Polar Year project, continuous ground penetrating radar (GPR) measurements were carried out during a traverse in Adelie Land (East Antarctica) during the 2008-2009 austral summer between the Italian-French Dome C (DC) polar plateau site and French Dumont D'Urville (DdU) coastal station. The aim of this study was to process and interpret GPR data in terms of snow accumulation, to analyse its spatial and temporal variability and compare it with historical data and modelling. The focus was on the last 300 yr, from the pre-industrial period to recent times. Beta-radioactivity counting and gamma spectrometry were applied to cores at the LGGE laboratory, providing a depth-age calibration for radar measurements. Over the 600 km of usable GPR data, depth and snow accumulation were determined with the help of three distinct layers visible on the radargrams (≈ 1730, 1799 and 1941 AD). Preliminary results reveal a gradual increase in accumulation towards the coast (from ≈ 3 cm w.e. a-1 at Dome C to ≈ 17 cm w.e. a-1 at the end of the transect) and previously undocumented undulating structures between 300 and 600 km from DC. Results agree fairly well with data from previous studies and modelling. Drawing final conclusions on temporal variations is difficult because of the margin of error introduced by density estimation. This study should have various applications, including model validation.

  19. Snow accumulation variability in Adelie Land (East Antarctica) derived from radar and firn core data. A 600 km transect from Dome C

    NASA Astrophysics Data System (ADS)

    Verfaillie, D.; Fily, M.; Le Meur, E.; Magand, O.; Jourdain, B.; Arnaud, L.; Favier, V.

    2012-07-01

    Polar ice sheets mass balance is a timely topic intensively studied in the context of global change and sea-level rise. However, obtaining mass balance estimates in Antarctica in particular, remains difficult due to various logistical problems. In the framework of the TASTE-IDEA program, labeled as an International Polar Year project, continuous Ground Penetrating Radar (GPR) measurements were carried out during a traverse realised in Adelie Land (East Antarctica) during the 2008-2009 austral summer between the Italo-French Dome C (DC) polar plateau site and French Dumont D'Urville (DdU) coastal station. The aim of this study was to process and interpret GPR data in terms of snow accumulation, to analyse its spatial and temporal variability along the DC-DdU traverse and compare it with historical data and modeling. The emphasis has been put on the last 300 yr, from the pre-industrial to recent time period. Beta-radioactivity counting and gamma spectrometry were studied in cores at LGGE laboratory, providing a depth-age calibration for radar measurements. Over the 600 km of usable GPR data, depth and snow accumulation were determined with the help of three distinct layers visible on the radargrams (≈1730, 1799 and 1941 AD). Preliminary results reveal a gradual accumulation increase towards the coast and the occurrence of previously undocumented undulating structures between 300 and 600 km from DC. Results agree fairly well with data from previous studies and modeling. Concluding on temporal variations is difficult because of the margin of error introduced by density estimation. This study should have various applications such as for model validation.

  20. Characterization of the l-alanine exporter AlaE of Escherichia coli and its potential role in protecting cells from a toxic-level accumulation of l-alanine and its derivatives.

    PubMed

    Kim, Seryoung; Ihara, Kohei; Katsube, Satoshi; Hori, Hatsuhiro; Ando, Tasuke; Isogai, Emiko; Yoneyama, Hiroshi

    2015-08-01

    We previously reported that the alaE gene of Escherichia coli encodes the l-alanine exporter AlaE. The objective of this study was to elucidate the mechanism of the AlaE exporter. The minimum inhibitory concentration of l-alanine and l-alanyl-l-alanine in alaE-deficient l-alanine-nonmetabolizing cells MLA301ΔalaE was 4- and >4000-fold lower, respectively, than in the alaE-positive parent cells MLA301, suggesting that AlaE functions as an efflux pump to avoid a toxic-level accumulation of intracellular l-alanine and its derivatives. Furthermore, the growth of the alaE-deficient mutant derived from the l-alanine-metabolizing strain was strongly inhibited in the presence of a physiological level of l-alanyl-l-alanine. Intact MLA301ΔalaE and MLA301ΔalaE/pAlaE cells producing plasmid-borne AlaE, accumulated approximately 200% and 50%, respectively, of the [(3) H]l-alanine detected in MLA301 cells, suggesting that AlaE exports l-alanine. When 200 mmol/L l-alanine-loaded inverted membrane vesicles prepared from MLA301ΔalaE/pAlaE were placed in a solution containing 200 mmol/L or 0.34 μmol/L l-alanine, energy-dependent [(3) H]l-alanine accumulation occurred under either condition. This energy-dependent uphill accumulation of [(3) H]l-alanine was strongly inhibited in the presence of carbonyl cyanide m-chlorophenylhydrazone but not by dicyclohexylcarbodiimide, suggesting that the AlaE-mediated l-alanine extrusion was driven by proton motive force. Based on these results, physiological roles of the l-alanine exporter are discussed.

  1. Stevia-derived compounds attenuate the toxic effects of ectopic lipid accumulation in the liver of obese mice: a transcriptomic and metabolomic study.

    PubMed

    Holvoet, Paul; Rull, Anna; García-Heredia, Anabel; López-Sanromà, Sílvia; Geeraert, Benjamine; Joven, Jorge; Camps, Jordi

    2015-03-01

    There is a close interaction between Type 2 Diabetes, obesity and liver disease. We have studied the effects of the two most abundant Stevia-derived steviol glycosides, stevioside and rebaudioside A, and their aglycol derivative steviol on liver steatosis and the hepatic effects of lipotoxicity using a mouse model of obesity and insulin resistance. We treated ob/ob and LDLR-double deficient mice with stevioside (10 mg⋅kg(-1)⋅day-1 p.o., n = 8), rebaudioside A (12 mg⋅kg(-1)⋅day-1 p.o., n = 8), or steviol (5 mg⋅kg(-1)⋅day(-1) p.o., n = 8). We determined their effects on liver steatosis and on the metabolic effects of lipotoxicity by histological analysis, and by combined gene-expression and metabolomic analyses. All compounds attenuated hepatic steatosis. This could be explained by improved glucose metabolism, fat catabolism, bile acid metabolism, and lipid storage and transport. We identified PPARs as important regulators and observed differences in effects on insulin resistance, inflammation and oxidative stress between Stevia-derived compounds. We conclude that Stevia-derived compounds reduce hepatic steatosis to a similar extent, despite differences in effects on glucose and lipid metabolism, and inflammation and oxidative stress. Thus our data show that liver toxicity can be reduced through several pathophysiological changes. Further identification of active metabolites and underlying mechanisms are warranted.

  2. Widespread occurrence and accumulation of bisphenol A diglycidyl ether (BADGE), bisphenol F diglycidyl ether (BFDGE) and their derivatives in human blood and adipose fat.

    PubMed

    Wang, Lei; Xue, Jingchuan; Kannan, Kurunthachalam

    2015-03-01

    Despite the widespread use of bisphenol A diglycidyl ether (BADGE) and bisphenol F diglycidyl ether (BFDGE) in various consumer products, studies on human exposure to these compounds are scarce. In this study, BADGE, BFDGE, and seven of their derivatives were determined in human adipose fat and blood plasma samples collected from New York City, NY. Bisphenol A bis (2,3-dihydroxypropyl) ether [BADGE·2H2O] was the major BADGE derivative found in 60% of the adipose samples and 70% of the plasma samples analyzed. High concentrations and detection frequencies of BFDGE were found in both adipose and plasma samples. BFDGE concentrations in adipose fat ranged from 19.1 to 4500 ng/g wet weight. A significant correlation between BADGE or BFDGE and their derivatives in adipose and plasma samples suggested hydration of these reactive compounds in humans. A significant positive correlation existed between BADGEs (i.e., the sum of BADGE and its five derivatives) and BFDGEs in adipose samples, which suggested similar exposure sources and pathways for these compounds in humans. Bisphenol A (BPA) also was analyzed in adipose fat and plasma, and its concentrations were positively correlated with those of BADGEs, which confirmed coexposure of BADGEs and BPA in humans.

  3. In-situ Measurements of Ozone Production Rates and Comparisons to Model-derived Production Rates During the Houston, TX and Denver, CO DISCOVER-AQ Campaigns

    NASA Astrophysics Data System (ADS)

    Baier, B. C.; Brune, W. H.; Miller, D. O.; Lefer, B. L.

    2015-12-01

    Tropospheric ozone (O3) is a secondary pollutant that has harmful effects on human and plant life. The climate and urban emissions in Houston, TX and Denver, CO can be conducive for significant ozone production and thus, high ozone events. Tighter government strategies for ozone mitigation have been proposed, which involve reducing the current EPA eight-hour ozone standard from 75 ppb to 65-70 ppb. These strategies rely on the reduction of ozone precursors in order to decrease the ozone production rate, P(O3). The changes in the ozone concentration at a certain location are dependent upon P(O3), so decreasing P(O3) can decrease ozone levels provided that it has not been transported from other areas. Air quality models test reduction strategies before they are implemented, locate ozone sources, and predict ozone episodes. Traditionally, P(O3) has been calculated by models. However, large uncertainties in model emissions inventories, chemical mechanisms, and meteorology can reduce confidence in this approach. A new instrument, the Measurement of Ozone Production Sensor (MOPS) directly measures P(O3) and can provide an alternate approach to determining P(O3). An updated version of the Penn State MOPS (MOPSv2.0) was deployed to Houston, TX and Denver, CO as a part of NASA's DISCOVER-AQ field campaign in the summers of 2013 and 2014, respectively. We present MOPS directly-measured P(O3) rates from these areas, as well as comparisons to zero-dimensional and three-dimensional modeled P(O3) using the RACM2 and MCMv2.2 mechanisms. These comparisons demonstrate the potential of the MOPS to test and evaluate model-derived P(O3), to advance the understanding of model chemical mechanisms, and to improve predictions of high ozone events.

  4. Slicing up the San Francisco Bay Area: Block kinematics and fault slip rates from GPS-derived surface velocities

    USGS Publications Warehouse

    d'Alessio, M. A.; Johanson, I.A.; Burgmann, R.; Schmidt, D.A.; Murray, M.H.

    2005-01-01

    Observations of surface deformation allow us to determine the kinematics of faults in the San Francisco Bay Area. We present the Bay Area velocity unification (BA??VU??, "bay view"), a compilation of over 200 horizontal surface velocities computed from campaign-style and continuous Global Positioning System (GPS) observations from 1993 to 2003. We interpret this interseismic velocity field using a three-dimensional block model to determine the relative contributions of block motion, elastic strain accumulation, and shallow aseismic creep. The total relative motion between the Pacific plate and the rigid Sierra Nevada/Great Valley (SNGV) microplate is 37.9 ?? 0.6 mm yr-1 directed toward N30.4??W ?? 0.8?? at San Francisco (??2??). Fault slip rates from our preferred model are typically within the error bounds of geologic estimates but provide a better fit to geodetic data (notable right-lateral slip rates in mm yr-1: San Gregorio fault, 2.4 ?? 1.0; West Napa fault, 4.0 ?? 3.0; zone of faulting along the eastern margin of the Coast Range, 5.4 ?? 1.0; and Mount Diablo thrust, 3.9 ?? 1.0 of reverse slip and 4.0 ?? 0.2 of right-lateral strike slip). Slip on the northern Calaveras is partitioned between both the West Napa and Concord/ Green Valley fault systems. The total convergence across the Bay Area is negligible. Poles of rotation for Bay Area blocks progress systematically from the North America-Pacific to North America-SNGV poles. The resulting present-day relative motion cannot explain the strike of most Bay Area faults, but fault strike does loosely correlate with inferred plate motions at the time each fault initiated. Copyright 2005 by the American Geophysical Union.

  5. Slicing up the San Francisco Bay Area: Block kinematics and fault slip rates from GPS-derived surface velocities

    NASA Astrophysics Data System (ADS)

    D'Alessio, M. A.; Johanson, I. A.; Bürgmann, R.; Schmidt, D. A.; Murray, M. H.

    2005-06-01

    Observations of surface deformation allow us to determine the kinematics of faults in the San Francisco Bay Area. We present the Bay Area velocity unification (B?V?, "bay view"), a compilation of over 200 horizontal surface velocities computed from campaign-style and continuous Global Positioning System (GPS) observations from 1993 to 2003. We interpret this interseismic velocity field using a three-dimensional block model to determine the relative contributions of block motion, elastic strain accumulation, and shallow aseismic creep. The total relative motion between the Pacific plate and the rigid Sierra Nevada/Great Valley (SNGV) microplate is 37.9 ± 0.6 mm yr-1 directed toward N30.4°W ± 0.8° at San Francisco (±2σ). Fault slip rates from our preferred model are typically within the error bounds of geologic estimates but provide a better fit to geodetic data (notable right-lateral slip rates in mm yr-1: San Gregorio fault, 2.4 ± 1.0; West Napa fault, 4.0 ± 3.0; zone of faulting along the eastern margin of the Coast Range, 5.4 ± 1.0; and Mount Diablo thrust, 3.9 ± 1.0 of reverse slip and 4.0 ± 0.2 of right-lateral strike slip). Slip on the northern Calaveras is partitioned between both the West Napa and Concord/Green Valley fault systems. The total convergence across the Bay Area is negligible. Poles of rotation for Bay Area blocks progress systematically from the North America-Pacific to North America-SNGV poles. The resulting present-day relative motion cannot explain the strike of most Bay Area faults, but fault strike does loosely correlate with inferred plate motions at the time each fault initiated.

  6. Accumulation of fallout radio nuclides such as cesium derived from Fukushima Dai-ichi NPP accident in reservoirs in Fukushima prefecture

    NASA Astrophysics Data System (ADS)

    Yoshimura, K.; Onda, Y.; Fukushima, T.

    2012-12-01

    A degree and condition of contamination of 137Cs and the other fallout radio nuclides (FRN) in reservoir can show large impacts on the irrigation and domestic/industrial water utilization. To clear the accumulation properties of FRN in the reservoirs, this study investigated the concentration, inventory and depth profile. Sediment was collected from four irrigation ponds and two sites in dam lake (Lake Horai) sited around Abukuma river system in summer 2011 (Jul.-Aug.), spring 2012 (Apr.) and summer 2012 (Aug.). Sediment collected by core sampler (diameter = 100 mm) was sliced into 20 mm thickness. Suspended solid (SS) in the water of Abukuma River was collected by SS sampler from Jul. 2011 to May 2012 with interval of two weeks. Samples were dried by oven (105°C, 24 hours) and grained. The radio activities were measured by gamma-ray detector (EGC 25-195-R, Canberra Eurisys, Meriden, U.S.A) equipped with a multichannel analyzer (DSA 1000, Canberra, Meriden, U.S.A). The concentration of 137Cs in Lake Horai showed non-uniform depth distribution, suggesting a large agitation of sediment and vertical migration of 137Cs. The concentrations of 137Cs in the sediment cores ranged from 47 to 63 kBq/kg in summer, 2011, and from 1 to 23 kBq/kg in spring, 2012. The concentration of 137Cs in SS in river water also decreased with time. The inventory drastically decreased with time, indicating that most of 137Cs in the sediment was flushed out from dam lake. In contrast to Lake Horai, most of 137Cs in the sediment of ponds were accumulated in surface layer, and the concentration decreased with increase in the depth. The concentration of 137Cs increased with time, resulted in the increase in inventory with time. We will be discussed about the seasonal trend in the FRN in the reservoirs.

  7. [Pandanus tectorius derived caffeoylquinic acids inhibit lipid accumulation in HepG2 hepatoma cells through regulation of gene expression involved in lipid metabolism].

    PubMed

    Wu, Chong-ming; Luan, Hong; Wang, Shuai; Zhang, Xiao-po; Liu, Hai-tao; Guo, Peng

    2015-03-01

    The fruit of Pandanus tectorius (PTF) has a long history of use as a folk medicine to treat hyperlipidemia in Hainan province, South China. Our previous studies have shown that the n-butanol extract of PTF is rich in caffeoylquinic acids and has an adequate therapeutic effect on dyslipidemic animals induced by high-fat diet. In this work, seven caffeoylquinic acids isolated from PTF were screened for the lipid-lowering activity in HepG2 hepatoma cells. Oil-Red O staining, microscopy and intracellular triglyceride (TG) and total cholesterol (TC) quantification showed that 3-O-caffeoylquinic acid (3-CQA), 3, 5-di-O-caffeoylquinic acid (3,5-CQA), and 3,4,5-tri-O-caffeoylquinic acid (3,4,5-CQA) significantly inhibited lipid accumulation induced by oleic acid and decreased intracellular levels of TC and TG in a dose-dependent manner. These three caffeoylquinic acids showed no significant cytotoxicity at concentrations of 1 -50 μmol x L(-1) as determined by MTT assay. Realtime quantitative PCR revealed that 3-CQA and 3, 5-CQA significantly increased the expression of lipid oxidation-related genes PPARα, CPT-1 and ACOX1 while 3-CQA, 3, 5-CQA and 3,4,5-CQA decreased the expression of lipogenic genes SREBP-1c, SREBP-2, HMGR, ACC, FAS. Overall, 3-CQA, 3, 5-CQA and 3, 4, 5-CQA may be the principal hypolipidemic components in PTF which can decrease intracellular lipid accumulation through up-regulating the expression of lipid oxidative genes and down-regulating the expression of lipogenic genes.

  8. An empirically derived basis for calculating the area, rate, and distribution of water-drop impingement on airfoils

    NASA Technical Reports Server (NTRS)

    Bergrun, Norman R

    1952-01-01

    An empirically derived basis for predicting the area, rate, and distribution of water-drop impingement on airfoils of arbitrary section is presented. The concepts involved represent an initial step toward the development of a calculation technique which is generally applicable to the design of thermal ice-prevention equipment for airplane wing and tail surfaces. It is shown that sufficiently accurate estimates, for the purpose of heated-wing design, can be obtained by a few numerical computations once the velocity distribution over the airfoil has been determined. The calculation technique presented is based on results of extensive water-drop trajectory computations for five airfoil cases which consisted of 15-percent-thick airfoils encompassing a moderate lift-coefficient range. The differential equations pertaining to the paths of the drops were solved by a differential analyzer.

  9. Heat accumulation during pulsed laser materials processing.

    PubMed

    Weber, Rudolf; Graf, Thomas; Berger, Peter; Onuseit, Volkher; Wiedenmann, Margit; Freitag, Christian; Feuer, Anne

    2014-05-01

    Laser materials processing with ultra-short pulses allows very precise and high quality results with a minimum extent of the thermally affected zone. However, with increasing average laser power and repetition rates the so-called heat accumulation effect becomes a considerable issue. The following discussion presents a comprehensive analytical treatment of multi-pulse processing and reveals the basic mechanisms of heat accumulation and its consequence for the resulting processing quality. The theoretical findings can explain the experimental results achieved when drilling microholes in CrNi-steel and for cutting of CFRP. As a consequence of the presented considerations, an estimate for the maximum applicable average power for ultra-shorts pulsed laser materials processing for a given pulse repetition rate is derived.

  10. Heat accumulator

    SciTech Connect

    Bracht, A.

    1981-09-29

    A heat accumulator comprises a thermally-insulated reservoir full of paraffin wax mixture or other flowable or meltable heat storage mass, heat-exchangers immersed in the mass, a heat-trap connected to one of the heat-exchangers, and a heat user connected to the other heat-exchanger. Pumps circulate fluids through the heat-trap and the heat-using means and the respective heat-exchangers, and a stirrer agitates and circulates the mass, and the pumps and the stirrer and electric motors driving these devices are all immersed in the mass.

  11. Using a detailed uncertainty analysis to adjust mapped rates of forest disturbance derived from Landsat time series data (Invited)

    NASA Astrophysics Data System (ADS)

    Cohen, W. B.; Yang, Z.; Stehman, S.; Huang, C.; Healey, S. P.

    2013-12-01

    Forest ecosystem process models require spatially and temporally detailed disturbance data to accurately predict fluxes of carbon or changes in biodiversity over time. A variety of new mapping algorithms using dense Landsat time series show great promise for providing disturbance characterizations at an annual time step. These algorithms provide unprecedented detail with respect to timing, magnitude, and duration of individual disturbance events, and causal agent. But all maps have error and disturbance maps in particular can have significant omission error because many disturbances are relatively subtle. Because disturbance, although ubiquitous, can be a relatively rare event spatially in any given year, omission errors can have a great impact on mapped rates. Using a high quality reference disturbance dataset, it is possible to not only characterize map errors but also to adjust mapped disturbance rates to provide unbiased rate estimates with confidence intervals. We present results from a national-level disturbance mapping project (the North American Forest Dynamics project) based on the Vegetation Change Tracker (VCT) with annual Landsat time series and uncertainty analyses that consist of three basic components: response design, statistical design, and analyses. The response design describes the reference data collection, in terms of the tool used (TimeSync), a formal description of interpretations, and the approach for data collection. The statistical design defines the selection of plot samples to be interpreted, whether stratification is used, and the sample size. Analyses involve derivation of standard agreement matrices between the map and the reference data, and use of inclusion probabilities and post-stratification to adjust mapped disturbance rates. Because for NAFD we use annual time series, both mapped and adjusted rates are provided at an annual time step from ~1985-present. Preliminary evaluations indicate that VCT captures most of the higher

  12. An "Aufbau" Approach to Understanding How the King-Altman Method of Deriving Rate Equations for Enzyme-Catalyzed Reactions Works

    ERIC Educational Resources Information Center

    Sims, Paul A.

    2009-01-01

    The King-Altman method of deriving rate equations for enzymatic reactions is applied to the derivation of the Michaelis-Menten equation, along with an explanation for how (or why) the King-Altman method works in this case. The slightly more complicated cases of competitive inhibition and a two-substrate enzyme-catalyzed reaction are then treated…

  13. Correlation between Change Rates of GRACE-derived Density and Ground-measured Temperature in Tibetan Plateau, 2004-2008

    NASA Astrophysics Data System (ADS)

    Zhang, K.; Ma, J.

    2011-12-01

    The amount of mountain glaciers on the Tibetan plateau accounts for 81.6% of the total glacial volume in China, and thus they become origination of Asia's seven major rivers including the Yangtze River, Yellow River, Ganges River, Indus River, Yarlung Zangbo River, and Nujiang River. Unfortunately, with the climate warming since the 20th century, most glaciers are retreating at a rate of 7.0 percent annually. The fast rate of glacier melt has meant more water runoff from the plateau which might exacerbate soil erosion, trigger drought and lead to sandstorms and desertification in the downstream regions. Thus, quantitive estimation and understanding of these glaciers' mass balance will be helpful to long-term sustainability of agriculture and hazard mitigation and give insights into climate change. As the primary factor for the glacier ice melting, the rising temperature should be correlated with the losing ice mass in the Tibetan plateau. However, quantification of glacier mass losses has been challenged, limited by temporarily and spatially sparse measurements using conventional data types and also due to the significant variations in glaciers' melting rates from region to region, and among glaciers in the same region. Nevertheless, the particular sensitivity of mountain glaciers to climate change makes it possible to estimate their mass change rates using GRACE even though the limited resolution. GRACE observations in southeastern Alaska, Patagonia and Tibet provided independent supports for the ability of glaciers' ice loss rates there (e.g., Matsuo and Heki, 2010). Here we expected to examine the correlation between Temperature Change Rates (TCRs) and Mass Change Rates (MCRs) and thus we computed the monthly density change and obtained the yearly Density Change Rates (DCRs) in Tibetan plateau of latitudes from 27N to 38N and longitudes ranging from 75E to 103E, and compared TCRs of ground-measured temperatures at 52 stations higher than 3000m with GRACE-derived

  14. Carbon-Coated, Diatomite-Derived Nanosilicon as a High Rate Capable Li-ion Battery Anode

    PubMed Central

    Campbell, Brennan; Ionescu, Robert; Tolchin, Maxwell; Ahmed, Kazi; Favors, Zachary; Bozhilov, Krassimir N.; Ozkan, Cengiz S.; Ozkan, Mihrimah

    2016-01-01

    Silicon is produced in a variety of ways as an ultra-high capacity lithium-ion battery (LIB) anode material. The traditional carbothermic reduction process required is expensive and energy-intensive; in this work, we use an efficient magnesiothermic reduction to convert the silica-based frustules within diatomaceous earth (diatomite, DE) to nanosilicon (nanoSi) for use as LIB anodes. Polyacrylic acid (PAA) was used as a binder for the DE-based nanoSi anodes for the first time, being attributed for the high silicon utilization under high current densities (up to 4C). The resulting nanoSi exhibited a high BET specific surface area of 162.6 cm2 g−1, compared to a value of 7.3 cm2 g−1 for the original DE. DE contains SiO2 architectures that make ideal bio-derived templates for nanoscaled silicon. The DE-based nanoSi anodes exhibit good cyclability, with a specific discharge capacity of 1102.1 mAh g−1 after 50 cycles at a C-rate of C/5 (0.7 A gSi−1) and high areal loading (2 mg cm−2). This work also demonstrates the fist rate capability testing for a DE-based Si anode; C-rates of C/30 - 4C were tested. At 4C (14.3 A gSi−1), the anode maintained a specific capacity of 654.3 mAh g−1 – nearly 2x higher than graphite’s theoretical value (372 mAh g−1). PMID:27713474

  15. Carbon-Coated, Diatomite-Derived Nanosilicon as a High Rate Capable Li-ion Battery Anode

    NASA Astrophysics Data System (ADS)

    Campbell, Brennan; Ionescu, Robert; Tolchin, Maxwell; Ahmed, Kazi; Favors, Zachary; Bozhilov, Krassimir N.; Ozkan, Cengiz S.; Ozkan, Mihrimah

    2016-10-01

    Silicon is produced in a variety of ways as an ultra-high capacity lithium-ion battery (LIB) anode material. The traditional carbothermic reduction process required is expensive and energy-intensive; in this work, we use an efficient magnesiothermic reduction to convert the silica-based frustules within diatomaceous earth (diatomite, DE) to nanosilicon (nanoSi) for use as LIB anodes. Polyacrylic acid (PAA) was used as a binder for the DE-based nanoSi anodes for the first time, being attributed for the high silicon utilization under high current densities (up to 4C). The resulting nanoSi exhibited a high BET specific surface area of 162.6 cm2 g‑1, compared to a value of 7.3 cm2 g‑1 for the original DE. DE contains SiO2 architectures that make ideal bio-derived templates for nanoscaled silicon. The DE-based nanoSi anodes exhibit good cyclability, with a specific discharge capacity of 1102.1 mAh g‑1 after 50 cycles at a C-rate of C/5 (0.7 A gSi‑1) and high areal loading (2 mg cm‑2). This work also demonstrates the fist rate capability testing for a DE-based Si anode; C-rates of C/30 - 4C were tested. At 4C (14.3 A gSi‑1), the anode maintained a specific capacity of 654.3 mAh g‑1 – nearly 2x higher than graphite’s theoretical value (372 mAh g‑1).

  16. Local SDSS galaxies in the Herschel Stripe 82 survey: a critical assessment of optically derived star formation rates

    NASA Astrophysics Data System (ADS)

    Rosario, D. J.; Mendel, J. T.; Ellison, S. L.; Lutz, D.; Trump, J. R.

    2016-04-01

    We study a set of 3319 galaxies in the redshift interval 0.04 < z < 0.15 with far-infrared (FIR) coverage from the Herschel Stripe 82 survey (HerS), and emission-line measurements, redshifts, stellar masses and star formation rates (SFRs) from the Sloan Digital Sky Survey (SDSS) (DR7) MPA/JHU data base. About 40 per cent of the sample are detected in the Herschel/SPIRE 250 μm band. Total infrared (TIR) luminosities derived from HerS and Wide-field Infrared Survey Explorer (WISE) photometry allow us to compare infrared and optical estimates of SFR with unprecedented statistics for diverse classes of galaxies. We find excellent agreement between TIR-derived and emission line-based SFRs for H II galaxies. Other classes, such as active galaxies and evolved galaxies, exhibit systematic discrepancies between optical and TIR SFRs. We demonstrate that these offsets are attributable primarily to survey biases and the large intrinsic uncertainties of the Dn4000- and colour-based optical calibrations used to estimate the SDSS SFRs of these galaxies. Using a classification scheme which expands upon popular emission-line methods, we demonstrate that emission-line galaxies with uncertain classifications include a population of massive, dusty, metal-rich star-forming systems that are frequently neglected in existing studies. We also study the capabilities of infrared selection of star-forming galaxies. FIR selection reveals a substantial population of galaxies dominated by cold dust which are missed by the long-wavelength WISE bands. Our results demonstrate that Herschel large-area surveys offer the means to construct large, relatively complete samples of local star-forming galaxies with accurate estimates of SFR that can be used to study the interplay between nuclear activity and star formation.

  17. Accumulation of VH Replacement Products in IgH Genes Derived from Autoimmune Diseases and Anti-Viral Responses in Human.

    PubMed

    Lange, Miles D; Huang, Lin; Yu, Yangsheng; Li, Song; Liao, Hongyan; Zemlin, Michael; Su, Kaihong; Zhang, Zhixin

    2014-01-01

    VH replacement refers to RAG-mediated secondary recombination of the IgH genes, which renews almost the entire VH gene coding region but retains a short stretch of nucleotides as a VH replacement footprint at the newly generated VH-DH junction. To explore the biological significance of VH replacement to the antibody repertoire, we developed a Java-based VH replacement footprint analyzer program and analyzed the distribution of VH replacement products in 61,851 human IgH gene sequences downloaded from the NCBI database. The initial assignment of the VH, DH, and JH gene segments provided a comprehensive view of the human IgH repertoire. To our interest, the overall frequency of VH replacement products is 12.1%; the frequencies of VH replacement products in IgH genes using different VH germline genes vary significantly. Importantly, the frequencies of VH replacement products are significantly elevated in IgH genes derived from different autoimmune diseases, including rheumatoid arthritis, systemic lupus erythematosus, and allergic rhinitis, and in IgH genes encoding various autoantibodies or anti-viral antibodies. The identified VH replacement footprints preferentially encoded charged amino acids to elongate IgH CDR3 regions, which may contribute to their autoreactivities or anti-viral functions. Analyses of the mutation status of the identified VH replacement products suggested that they had been actively involved in immune responses. These results provide a global view of the distribution of VH replacement products in human IgH genes, especially in IgH genes derived from autoimmune diseases and anti-viral immune responses.

  18. Fault slip-rates derived from modeling of on-shore marine terraces in the western Corinth Gulf

    NASA Astrophysics Data System (ADS)

    Palyvos, Nikos; De Martini, Paolo Marco; Mancini, Marco; Pantosti, Daniela

    2013-04-01

    Data available for estimating fault slip-rates from accurate modeling of uplifted on-shore marine terraces although limited are all derivatives of the research activity performed from 2004 to 2007 within the 3HAZ-Corinth project. We concentrated our efforts in the Aravonitsa area where we had a nicely preserved staircase of marine terraces. In particular, we recognized and mapped in detail all the marine terraces by qualitative DEM analysis, airphoto interpretation and field survey and we adopted a forward modeling procedure to fit the data. The modeling approach we used in this work does not take into account any effect related to sedimentation, compaction and erosion nor any interseismic adjustment and thus, being purely based on coseismic deformation, the obtained results should be considered as maxima. In the study area we were able to recognize several surfaces related to sea-level still stands. Their areal distribution and elevation are strongly influenced by past intense erosion on the underlying weakly consolidated sediments, and by the activity of secondary faults at the footwall of the Neos Erineos Fault, being part of the Lambiri - Neos Erineos - Aigion Fault zone (LANEfz). The Neos Erineos Fault has been studied and investigated in details and it appears as one of the main N-dipping normal faults bounding the southern shore of the Corinth Gulf and taking on part of the observed N-S striking extension. U/Th-series age dates and nannoplankton analyses, performed on selected samples collected at different heights on the studied surfaces, allowed us to to reconstruct an almost complete and chronologically well constrained transect of uplifted marine terraces belonging to the Late Quaternary (as old as 350 ka). A tentative correlation with marine isotopic stages (MIS) and specifically with main highstands from the Late Quaternary eustatic sea-level curve was attempted in order to calculate footwall uplift rate for the Neos Erineos Fault. The calculated

  19. 34 CFR 222.69 - What tax rates may the Secretary use if substantial local revenues are derived from local tax...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false What tax rates may the Secretary use if substantial local revenues are derived from local tax sources other than real property taxes? 222.69 Section 222.69... Educational Agencies Under Section 8003(f) of the Act § 222.69 What tax rates may the Secretary use...

  20. Derivation of Failure Rates and Probability of Failures for the International Space Station Probabilistic Risk Assessment Study

    NASA Technical Reports Server (NTRS)

    Vitali, Roberto; Lutomski, Michael G.

    2004-01-01

    National Aeronautics and Space Administration s (NASA) International Space Station (ISS) Program uses Probabilistic Risk Assessment (PRA) as part of its Continuous Risk Management Process. It is used as a decision and management support tool to not only quantify risk for specific conditions, but more importantly comparing different operational and management options to determine the lowest risk option and provide rationale for management decisions. This paper presents the derivation of the probability distributions used to quantify the failure rates and the probability of failures of the basic events employed in the PRA model of the ISS. The paper will show how a Bayesian approach was used with different sources of data including the actual ISS on orbit failures to enhance the confidence in results of the PRA. As time progresses and more meaningful data is gathered from on orbit failures, an increasingly accurate failure rate probability distribution for the basic events of the ISS PRA model can be obtained. The ISS PRA has been developed by mapping the ISS critical systems such as propulsion, thermal control, or power generation into event sequences diagrams and fault trees. The lowest level of indenture of the fault trees was the orbital replacement units (ORU). The ORU level was chosen consistently with the level of statistically meaningful data that could be obtained from the aerospace industry and from the experts in the field. For example, data was gathered for the solenoid valves present in the propulsion system of the ISS. However valves themselves are composed of parts and the individual failure of these parts was not accounted for in the PRA model. In other words the failure of a spring within a valve was considered a failure of the valve itself.

  1. Study of High Etch Rate Bottom Antireflective Coating and Gap Fill Materials Using Dextrin Derivatives in ArF Lithography

    NASA Astrophysics Data System (ADS)

    Takei, Satoshi; Shinjo, Tetsuya; Sakaida, Yasushi

    2007-11-01

    In the present paper, we describe a novel class of bottom antireflective coating (BARC) and gap fill materials using dextrin with a-glycoside bonds in a polysaccharide. ArF resist underlayer materials containing a dextrin ester polymer for lithography were studied. Dextrin is a high molecular weight compound with several hydroxyl groups and a low solubility in resist and BARC solvents. Therefore, it is difficult to use dextrin polymers in resist underlayer materials such as BARC and gap fill materials. The main polymer needs to be soluble in propylene glycol monomethylether, propylene glycol monomethylether acetate, and ethyl lactate as common solvents to avoid the issue of defects in the coater cup due to incompatability. The dextrin ester polymer in this study was obtained by the esterification of the hydroxyl groups of dextrin resulting in improved solubility of these organic solvents. The etch rate of the new BARC and gap fill materials of the dextrin ester polymers was more than twofold faster than the etch rate of the ArF resists evaluated under a CF4 gas condition using reactive ion etching. The improved etch performance was also verified by comparison with poly(4-hydroxystyrene) and poly(2-hydroxypropyl methacrylate) as references. In addition to the superior etch performance, these materials showed good resist profiles and via filling performance without voids in via holes. On the basis of our findings, this technology of using the novel dextrin derivatives as sacrificial materials under a resist can be applied in devices of 45 nm node and higher.

  2. Evaluating Individual Training Adaptation With Smartphone-Derived Heart Rate Variability in a Collegiate Female Soccer Team.

    PubMed

    Flatt, Andrew A; Esco, Michael R

    2016-02-01

    Monitoring individual responses throughout training may provide insight to coaches regarding how athletes are coping to the current program. It is unclear if the evolution of heart rate variability (HRV) throughout training in team-sport athletes can be useful in providing early indications of individual adaptation. This study evaluated relationships between changes in resting cardiac autonomic markers derived from a novel smartphone device within the first 3 weeks of a 5-week conditioning program and the eventual change in intermittent running performance at week 5 among 12 collegiate female soccer players. Change variables from weeks 1 to 3 of the weekly mean and weekly coefficient of variation for resting heart rate ([INCREMENT]RHRmean and [INCREMENT]RHRcv, respectively) and log-transformed root mean square of successive R-R intervals multiplied by 20 ([INCREMENT]Ln rMSSDmean and [INCREMENT]Ln rMSSDcv, respectively) were compared with changes in Yo-Yo Intermittent Recovery Test Level 1 performance ([INCREMENT]Yo-Yo). A very large and significant correlation was found between [INCREMENT]Yo-Yo and [INCREMENT]Ln rMSSDcv (r = -0.74; p = <0.01) and a large nonsignificant correlation was found with [INCREMENT]Ln rMSSDmean (r = 0.50; p = 0.096). This study suggests that a decrease in Ln rMSSDcv within the first 3 weeks of training is a favorable response, indicative of positive adaptation. Collecting daily HRV data with a smartphone application using ultrashort HRV measures seems useful for athlete monitoring. PMID:26200192

  3. Relationship between sedimentation rates and benthic impact on Maërl beds derived from fish farming in the Mediterranean.

    PubMed

    Sanz-Lázaro, Carlos; Belando, María Dolores; Marín-Guirao, Lázaro; Navarrete-Mier, Francisco; Marín, Arnaldo

    2011-02-01

    The aim of this work was to study the dispersion of particulate wastes derived from marine fish farming and correlate the data with the impact on the seabed. Carbon and nutrients were correlated with the physico-chemical parameters of the sediment and the benthic community structure. The sedimentation rates in the benthic system were 1.09, 0.09 and 0.13 g m⁻² day⁻¹ for particulate organic carbon (POC), particulate organic nitrogen (PON) and total phosphorus (TP), respectively. TP was a reliable parameter for establishing the spatial extent of the fish farm particulate wastes. Fish farming was seen to influence not only physico-chemical and biological parameters but also the functioning of the ecosystem from a trophic point of view, particularly affecting the grazers and the balance among the trophic groups. POC, PON and TP sedimentation dynamics reflected the physico-chemical status of the sediment along the distance gradient studied, while their impact on the benthic community extended further. Therefore, the level of fish farm impact on the benthic community might be underestimated if it is assessed by merely taking into account data obtained from waste dispersion rates. The benthic habitat beneath the fish farm, Maërl bed, was seen to be very sensitive to aquaculture impact compared with other unvegetated benthic habitats, with an estimated POC-carrying capacity to maintain current diversity of 0.087 g C m⁻² day⁻¹ (only 36% greater than the basal POC input). Environmental protection agencies should define different aquaculture waste load thresholds for different benthic communities affected by finfish farming, according to their particular degree of sensitivity, in order to maintain natural ecosystem functions.

  4. Non-Saccharomyces and Saccharomyces strains co-fermentation increases acetaldehyde accumulation: effect on anthocyanin-derived pigments in Tannat red wines.

    PubMed

    Medina, Karina; Boido, Eduardo; Fariña, Laura; Dellacassa, Eduardo; Carrau, Francisco

    2016-07-01

    During fermentation, Saccharomyces cerevisiae releases into the medium secondary metabolic products, such as acetaldehyde, able to react with anthocyanins, producing more stable derived pigments. However, very limited reports are found about non-Saccharomyces effects on grape fermentation. In this study, six non-Saccharomyces yeast strains, belonging to the genera Metschnikowia and Hanseniaspora, were screened for their effect on red wine colour and wine-making capacity under pure culture conditions and mixed with Saccharomyces. An artificial red grape must was prepared, containing a phenolic extract of Tannat grapes that allows monitoring changes of key phenol parameters during fermentation, but without skin solids in the medium. When fermented in pure cultures, S. cerevisiae produced higher concentrations of acetaldehyde and vitisin B (acetaldehyde reaction-dependent) compared to M. pulcherrima M00/09G, Hanseniaspora guillermondii T06/09G, H. opuntiae T06/01G, H. vineae T02/05F and H. clermontiae (A10/82Fand C10/54F). However, co-fermentation of H. vineae and H. clermontiae with S. cerevisiae resulted in a significantly higher concentration of acetaldehyde compared with the pure S. cerevisiae control. HPLC-DAD-MS analysis confirmed an increased formation of vitisin B in co-fermentation treatments when compared to pure Saccharomyces fermentation, suggesting the key role of acetaldehyde. Copyright © 2016 John Wiley & Sons, Ltd. PMID:26888345

  5. Non-Saccharomyces and Saccharomyces strains co-fermentation increases acetaldehyde accumulation: effect on anthocyanin-derived pigments in Tannat red wines.

    PubMed

    Medina, Karina; Boido, Eduardo; Fariña, Laura; Dellacassa, Eduardo; Carrau, Francisco

    2016-07-01

    During fermentation, Saccharomyces cerevisiae releases into the medium secondary metabolic products, such as acetaldehyde, able to react with anthocyanins, producing more stable derived pigments. However, very limited reports are found about non-Saccharomyces effects on grape fermentation. In this study, six non-Saccharomyces yeast strains, belonging to the genera Metschnikowia and Hanseniaspora, were screened for their effect on red wine colour and wine-making capacity under pure culture conditions and mixed with Saccharomyces. An artificial red grape must was prepared, containing a phenolic extract of Tannat grapes that allows monitoring changes of key phenol parameters during fermentation, but without skin solids in the medium. When fermented in pure cultures, S. cerevisiae produced higher concentrations of acetaldehyde and vitisin B (acetaldehyde reaction-dependent) compared to M. pulcherrima M00/09G, Hanseniaspora guillermondii T06/09G, H. opuntiae T06/01G, H. vineae T02/05F and H. clermontiae (A10/82Fand C10/54F). However, co-fermentation of H. vineae and H. clermontiae with S. cerevisiae resulted in a significantly higher concentration of acetaldehyde compared with the pure S. cerevisiae control. HPLC-DAD-MS analysis confirmed an increased formation of vitisin B in co-fermentation treatments when compared to pure Saccharomyces fermentation, suggesting the key role of acetaldehyde. Copyright © 2016 John Wiley & Sons, Ltd.

  6. C02(nu2)-0 Quenching Rate Coefficient Derived from Coincidental Fort Collins Lidar and SABER Measurements

    NASA Technical Reports Server (NTRS)

    Feofilov, A. G.; Kutepov, A. A.; She, C. Y.; Smith, A. K.; Pesnell, W. D.; Goldberg, R. A.

    2009-01-01

    Among the processes governing the energy balance in the mesosphere and lower thermosphere (MLT), the quenching of CO2(V2) vibrational levels in collisions with oxygen atoms plays an important role. However, neither the rate coefficient of this process (k(CO2O)) nor the atomic oxygen concentrations ([O]) in the MLT are well known. The discrepancy between k(CO2O) measured in the lab and retrieved from atmospheric measurements is of about factor of 2.5. At the same time, the discrepancy between [O] in the MLT measured by different instruments is of the same order of magnitude. In this work we used a synergy of a ground based lidar and satellite infrared radiometer to make a further step in understanding of the physics of the region. In this study we apply the night- and daytime temperatures between 80 and 110 km measured by the Colorado State University narrow-band sodium (Na) lidar located at Fort Collins, Colorado for retrieving the product of k(CO2-O) x [O] from the limb radiances in the 15 micron channel measured by the SABER/TIMED instrument for nearly simultaneous common volume measurements of both instruments within +/-1 degree in latitude, +/-2 degrees in longitude and +/-10 minutes in time. We derive k(CO2-O) and its possible variation range from the retrieved product by utilizing the [O] values measured by the SABER and other instruments.

  7. Accelerated mutation accumulation in asexual lineages of a freshwater snail.

    PubMed

    Neiman, Maurine; Hehman, Gery; Miller, Joseph T; Logsdon, John M; Taylor, Douglas R

    2010-04-01

    Sexual reproduction is both extremely costly and widespread relative to asexual reproduction, meaning that it must also confer profound advantages in order to persist. One theorized benefit of sex is that it facilitates the clearance of harmful mutations, which would accumulate more rapidly in the absence of recombination. The extent to which ineffective purifying selection and mutation accumulation are direct consequences of asexuality and whether the accelerated buildup of harmful mutations in asexuals can occur rapidly enough to maintain sex within natural populations, however, remain as open questions. We addressed key components of these questions by estimating the rate of mutation accumulation in the mitochondrial genomes of multiple sexual and asexual representatives of Potamopyrgus antipodarum, a New Zealand snail characterized by mixed sexual/asexual populations. We found that increased mutation accumulation is associated with asexuality and occurs rapidly enough to be detected in recently derived asexual lineages of P. antipodarum. Our results demonstrate that increased mutation accumulation in asexuals can differentially affect coexisting and ecologically similar sexual and asexual lineages. The accelerated rate of mutation accumulation observed in asexual P. antipodarum provides some of the most direct evidence to date for a link between asexuality and mutation accumulation and implies that mutational buildup could be rapid enough to contribute to the short-term evolutionary mechanisms that favor sexual reproduction.

  8. Assessing the Sensitivity of Satellite-Derived Estimates of Ice Sheet Mass Balance to Regional Climate Model Simulations of Snow Accumulation and Firn Compaction

    NASA Astrophysics Data System (ADS)

    Briggs, K.; Shepherd, A.; Horwath, M.; Horvath, A.; Nagler, T.; Wuite, J.; Muir, A.; Gilbert, L.; Mouginot, J.

    2015-12-01

    Surface mass balance (SMB) estimates from Regional Climate Models (RCMs) are fundamental for assessing and understanding ice sheet mass trends. Mass budget and altimetry assessments rely on RCMs both directly for estimates of the SMB contribution to the total mass trend, and indirectly for ancillary data in the form of firn compaction corrections. As such, mass balance assessments can be highly sensitive to RCM outputs and therefore their accuracy. Here we assess the extent to which geodetic measurements of mass balance are sensitive to RCM model outputs at different resolutions. We achieve this by comparing SMB dependent estimates of mass balance from the mass budget method and altimetry, with those from satellite gravimetry that are independent of SMB estimates. Using the outputs of the RACMO/ANT 2.3 model at 5.5 km and 27 km horizontal spatial resolution, we generate estimates of mass balance using the mass budget method and altimetry for the Western Palmer Land region of the Antarctic Peninsula between 2003 and 2014. We find a 19% increase in the long-term (1980 to 2014) mean annual SMB for the region when enhancing the model resolution to 5.5 km. This translates into an approximate 50% reduction in the total mass loss from 2003 to 2014 calculated with the mass budget method and a 15% increase in the altimetry estimate. The use of the enhanced resolution product leads to consistency between the estimates of mass loss from the altimetry and the mass budget method that is not observed with the coarser resolution product, in which estimates of cumulative mass fall beyond the relative errors. Critically, when using the 5.5 km product, we find excellent agreement, both in pattern and magnitude, with the independent estimate derived from gravimetry. Our results point toward the crucial need for high resolution SMB products from RCMs for mass balance assessments, particularly in regions of high mass turnover and complex terrain as found over the Antarctic Peninsula.

  9. Nitrogen Limited Red and Green Leaf Lettuce Accumulate Flavonoid Glycosides, Caffeic Acid Derivatives, and Sucrose while Losing Chlorophylls, Β-Carotene and Xanthophylls.

    PubMed

    Becker, Christine; Urlić, Branimir; Jukić Špika, Maja; Kläring, Hans-Peter; Krumbein, Angelika; Baldermann, Susanne; Goreta Ban, Smiljana; Perica, Slavko; Schwarz, Dietmar

    2015-01-01

    Reduction of nitrogen application in crop production is desirable for ecological and health-related reasons. Interestingly, nitrogen deficiency can lead to enhanced concentrations of polyphenols in plants. The reason for this is still under discussion. The plants' response to low nitrogen concentration can interact with other factors, for example radiation intensity. We cultivated red and green leaf lettuce hydroponically in a Mediterranean greenhouse, supplying three different levels of nitrogen (12 mM, 3 mM, 0.75 mM), either in full or reduced (-50%) radiation intensity. In both red and green lettuce, we found clear effects of the nitrogen treatments on growth characteristics, phenolic and photosynthetic compounds, nitrogen, nitrate and carbon concentration of the plants. Interestingly, the concentrations of all main flavonoid glycosides, caffeic acid derivatives, and sucrose increased with decreasing nitrogen concentration, whereas those of chlorophylls, β-carotene, neoxanthin, lactucaxanthin, all trans- and cis-violaxanthin decreased. The constitutive concentrations of polyphenols were lower in the green cultivar, but their relative increase was more pronounced than in the red cultivar. The constitutive concentrations of chlorophylls, β-carotene, neoxanthin, all trans- and cis-violaxanthin were similar in red and green lettuce and with decreasing nitrogen concentration they declined to a similar extent in both cultivars. We only detected little influence of the radiation treatments, e.g. on anthocyanin concentration, and hardly any interaction between radiation and nitrogen concentration. Our results imply a greater physiological plasticity of green compared to the red lettuce regarding its phenolic compounds. They support the photoprotection theory regarding anthocyanins as well as the theory that the deamination activity of phenylalanine ammonia-lyase drives phenylpropanoid synthesis.

  10. Nitrogen Limited Red and Green Leaf Lettuce Accumulate Flavonoid Glycosides, Caffeic Acid Derivatives, and Sucrose while Losing Chlorophylls, Β-Carotene and Xanthophylls.

    PubMed

    Becker, Christine; Urlić, Branimir; Jukić Špika, Maja; Kläring, Hans-Peter; Krumbein, Angelika; Baldermann, Susanne; Goreta Ban, Smiljana; Perica, Slavko; Schwarz, Dietmar

    2015-01-01

    Reduction of nitrogen application in crop production is desirable for ecological and health-related reasons. Interestingly, nitrogen deficiency can lead to enhanced concentrations of polyphenols in plants. The reason for this is still under discussion. The plants' response to low nitrogen concentration can interact with other factors, for example radiation intensity. We cultivated red and green leaf lettuce hydroponically in a Mediterranean greenhouse, supplying three different levels of nitrogen (12 mM, 3 mM, 0.75 mM), either in full or reduced (-50%) radiation intensity. In both red and green lettuce, we found clear effects of the nitrogen treatments on growth characteristics, phenolic and photosynthetic compounds, nitrogen, nitrate and carbon concentration of the plants. Interestingly, the concentrations of all main flavonoid glycosides, caffeic acid derivatives, and sucrose increased with decreasing nitrogen concentration, whereas those of chlorophylls, β-carotene, neoxanthin, lactucaxanthin, all trans- and cis-violaxanthin decreased. The constitutive concentrations of polyphenols were lower in the green cultivar, but their relative increase was more pronounced than in the red cultivar. The constitutive concentrations of chlorophylls, β-carotene, neoxanthin, all trans- and cis-violaxanthin were similar in red and green lettuce and with decreasing nitrogen concentration they declined to a similar extent in both cultivars. We only detected little influence of the radiation treatments, e.g. on anthocyanin concentration, and hardly any interaction between radiation and nitrogen concentration. Our results imply a greater physiological plasticity of green compared to the red lettuce regarding its phenolic compounds. They support the photoprotection theory regarding anthocyanins as well as the theory that the deamination activity of phenylalanine ammonia-lyase drives phenylpropanoid synthesis. PMID:26569488

  11. Nitrogen Limited Red and Green Leaf Lettuce Accumulate Flavonoid Glycosides, Caffeic Acid Derivatives, and Sucrose while Losing Chlorophylls, Β-Carotene and Xanthophylls

    PubMed Central

    Becker, Christine; Urlić, Branimir; Jukić Špika, Maja; Kläring, Hans-Peter; Krumbein, Angelika; Baldermann, Susanne; Goreta Ban, Smiljana; Perica, Slavko; Schwarz, Dietmar

    2015-01-01

    Reduction of nitrogen application in crop production is desirable for ecological and health-related reasons. Interestingly, nitrogen deficiency can lead to enhanced concentrations of polyphenols in plants. The reason for this is still under discussion. The plants’ response to low nitrogen concentration can interact with other factors, for example radiation intensity. We cultivated red and green leaf lettuce hydroponically in a Mediterranean greenhouse, supplying three different levels of nitrogen (12 mM, 3 mM, 0.75 mM), either in full or reduced (-50%) radiation intensity. In both red and green lettuce, we found clear effects of the nitrogen treatments on growth characteristics, phenolic and photosynthetic compounds, nitrogen, nitrate and carbon concentration of the plants. Interestingly, the concentrations of all main flavonoid glycosides, caffeic acid derivatives, and sucrose increased with decreasing nitrogen concentration, whereas those of chlorophylls, β-carotene, neoxanthin, lactucaxanthin, all trans- and cis-violaxanthin decreased. The constitutive concentrations of polyphenols were lower in the green cultivar, but their relative increase was more pronounced than in the red cultivar. The constitutive concentrations of chlorophylls, β-carotene, neoxanthin, all trans- and cis-violaxanthin were similar in red and green lettuce and with decreasing nitrogen concentration they declined to a similar extent in both cultivars. We only detected little influence of the radiation treatments, e.g. on anthocyanin concentration, and hardly any interaction between radiation and nitrogen concentration. Our results imply a greater physiological plasticity of green compared to the red lettuce regarding its phenolic compounds. They support the photoprotection theory regarding anthocyanins as well as the theory that the deamination activity of phenylalanine ammonia-lyase drives phenylpropanoid synthesis. PMID:26569488

  12. Uptake of a fluorescent L-glucose derivative 2-NBDLG into three-dimensionally accumulating insulinoma cells in a phloretin-sensitive manner.

    PubMed

    Sasaki, Ayako; Nagatomo, Katsuhiro; Ono, Koki; Yamamoto, Toshihiro; Otsuka, Yuji; Teshima, Tadashi; Yamada, Katsuya

    2016-01-01

    Of two stereoisomers of glucose, only D- and not L-glucose is abundantly found in nature, being utilized as an essential fuel by most organisms. The uptake of D-glucose into mammalian cells occurs through glucose transporters such as GLUTs, and this process has been effectively monitored by a fluorescent D-glucose derivative 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG) at the single cell level. However, since fluorescence is an arbitrary measure, we have developed a fluorescent analog of L-glucose 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-L-glucose (2-NBDLG), as a negative control substrate for more accurately identifying the stereoselectivity of the uptake. Interestingly, a small portion of mouse insulinoma cells MIN6 abundantly took up 2-NBDLG at a late culture stage (≳ 10 days in vitro, DIV) when multi-cellular spheroids exhibiting heterogeneous nuclei were formed, whereas no such uptake was detected at an early culture stage (≲ 6 DIV). The 2-NBDLG uptake was persistently observed in the presence of a GLUT inhibitor cytochalasin B. Neither D- nor L-glucose in 50 mM abolished the uptake. No significant inhibition was detected by inactivating sodium/glucose cotransporters (SGLTs) with Na(+)-free condition. To our surprise, the 2-NBDLG uptake was totally inhibited by phloretin, a broad spectrum inhibitor against transporters/channels including GLUTs and aquaporins. From these, a question might be raised if non-GLUT/non-SGLT pathways participate in the 2-NBDLG uptake into spheroid-forming MIN6 insulinoma. It might also be worthwhile investigating whether 2-NBDLG can be used as a functional probe for detecting cancer, since the nuclear heterogeneity is among critical features of malignancy. PMID:26553070

  13. Ultraviolet to infrared emission of z > 1 galaxies: Can we derive reliable star formation rates and stellar masses?

    NASA Astrophysics Data System (ADS)

    Buat, V.; Heinis, S.; Boquien, M.; Burgarella, D.; Charmandaris, V.; Boissier, S.; Boselli, A.; Le Borgne, D.; Morrison, G.

    2014-01-01

    Aims: Our knowledge of the cosmic mass assembly relies on measurements of star formation rates (SFRs) and stellar masses (Mstar), of galaxies as a function of redshift. These parameters must be estimated in a consistent way with a good knowledge of systematics before studying their correlation and the variation of the specific SFR. Constraining these fundamental properties of galaxies across the Universe is of utmost importance if we want to understand galaxy formation and evolution. Methods: We seek to derive SFRs and stellar masses in distant galaxies and to quantify the main uncertainties affecting their measurement. We explore the impact of the assumptions made in their derivation with standard calibrations or through a fitting process, as well as the impact of the available data, focusing on the role of infrared emission originating from dust. Results: We build a sample of galaxies with z > 1, all observed from the ultraviolet to the infrared in their rest frame. The data are fitted with the code CIGALE, which is also used to build and analyse a catalogue of mock galaxies. Models with different star formation histories are introduced: an exponentially decreasing or increasing SFR and a more complex one coupling a decreasing SFR with a younger burst of constant star formation. We define different sets of data, with or without a good sampling of the ultraviolet range, near-infrared, and thermal infrared data. Variations of the metallicity are also investigated. The impact of these different cases on the determination of stellar mass and SFR are analysed. Conclusions: Exponentially decreasing models with a redshift formation of the stellar population zf ≃ 8 cannot fit the data correctly. All the other models fit the data correctly at the price of unrealistically young ages when the age of the single stellar population is taken to be a free parameter, especially for the exponentially decreasing models. The best fits are obtained with two stellar populations. As

  14. High Potency of a Novel Resveratrol Derivative, 3,3',4,4'-Tetrahydroxy-trans-stilbene, against Ovarian Cancer Is Associated with an Oxidative Stress-Mediated Imbalance between DNA Damage Accumulation and Repair.

    PubMed

    Mikuła-Pietrasik, Justyna; Sosińska, Patrycja; Murias, Marek; Wierzchowski, Marcin; Brewińska-Olchowik, Marta; Piwocka, Katarzyna; Szpurek, Dariusz; Książek, Krzysztof

    2015-01-01

    We explored the effect of a new resveratrol (RVT) derivative, 3,3',4,4'-tetrahydroxy-trans-stilbene (3,3',4,4'-THS), on viability, apoptosis, proliferation, and senescence of three representative lines of ovarian cancer cells, that is, A2780, OVCAR-3, and SKOV-3, in vitro. In addition, the mechanistic aspects of 3,3',4,4'-THS activity, including cell redox homeostasis (the production of reactive oxygen species, activity of enzymatic antioxidants, and magnitude of DNA damage accumulation and repair), and the activity of caspases (3, 8, and 9) and p38 MAPK were examined. The study showed that 3,3',4,4'-THS affects cancer cell viability much more efficiently than its parent drug. This effect coincided with increased generation of reactive oxygen species, downregulated activity of superoxide dismutase and catalase, and excessive accumulation of 8-hydroxy-2'-deoxyguanosine and its insufficient repair due to decreased expression of DNA glycosylase I. Cytotoxicity elicited by 3,3',4,4'-THS was related to increased incidence of apoptosis, which was mediated by caspases 3 and 9. Moreover, 3,3',4,4'-THS inhibited cancer cell proliferation and accelerated senescence, which was accompanied by the activation of p38 MAPK. Collectively, our findings indicate that 3,3',4,4'-THS may constitute a valuable tool in the fight against ovarian malignancy and that the anticancer capabilities of this stilbene proceed in an oxidative stress-dependent mechanism. PMID:26229578

  15. A new 2α,5α,10β,14β-tetraacetoxy-4(20),11-taxadiene (SIA) derivative overcomes paclitaxel resistance by inhibiting MAPK signaling and increasing paclitaxel accumulation in breast cancer cells.

    PubMed

    Mei, Mei; Xie, Dan; Zhang, Yi; Jin, Jing; You, Feng; Li, Yan; Dai, Jungui; Chen, Xiaoguang

    2014-01-01

    Tumor resistance due to multiple mechanisms seriously hinders the efficacy of chemotherapy drugs such as paclitaxel. The most widely studied P-glycoprotein inhibitors still have limited ability to reverse resistance in the clinic. In this study, NPB304, a novel Sinenxan A (SIA) derivative, was found to significantly sensitize resistant breast cancer cells to paclitaxel in vitro and in vivo. Treatment with NPB304 increased paclitaxel-induced apoptosis in a p53-dependent manner through PARP cleavage. Importantly, NPB304 enhanced the antitumor effect of paclitaxel in resistant breast tumor xenografts in nude mice without significantly affecting weight loss. NPB304 regulated cell resistance through inhibition of MAPK pathway components, including p-ERK and p-p38. Moreover, NPB304 increased paclitaxel accumulation by affecting P-gp function. In addition to increasing Rhodamine 123 accumulation, NPB304 promoted bidirectional permeability but decreased the efflux ratio of paclitaxel in a Caco-2 monolayer model, thereby increasing the intracellular concentration of paclitaxel. Similarly, NPB304 increased the concentration of paclitaxel in the resistant tumor tissue. Hence, NPB304 is a novel compound that promotes the sensitization of resistant cells to paclitaxel through multiple mechanisms and has the potential for use in combination therapies to treat resistant breast cancer.

  16. Combined Aircraft and Satellite-Derived Storm Electric Current and Lightning Rates Measurements and Implications for the Global Electric Circuit

    NASA Technical Reports Server (NTRS)

    Mach, Douglas M.; Blakeslee, Richard J.; Bateman, Monte G.

    2010-01-01

    Using rotating vane electric field mills and Gerdien capacitors, we measured the electric field profile and conductivity during 850 overflights of electrified shower clouds and thunderstorms spanning regions including the Southeastern United States, the Western Atlantic Ocean, the Gulf of Mexico, Central America and adjacent oceans, Central Brazil, and the South Pacific. The overflights include storms over land and ocean, with and without lightning, and with positive and negative fields above the storms. The measurements were made with the NASA ER-2 and the Altus-II high altitude aircrafts. Peak electric fields, with lightning transients removed, ranged from -1.0 kV/m to 16 kV/m, with a mean value of 0.9 kV/m. The median peak field was 0.29 kV/m. Integrating our electric field and conductivity data, we determined total conduction currents and flash rates for each overpass. With knowledge of the storm location (land or ocean) and type (with or without lightning), we determine the mean currents by location and type. The mean current for ocean storms with lightning is 1.6 A while the mean current for land storms with lightning is 1.0 A. The mean current for oceanic storms without lightning (i.e., electrified shower clouds) is 0.39 A and the mean current for land storms without lightning is 0.13 A. Thus, on average, land storms with or without lightning have about half the mean current as their corresponding oceanic storm counterparts. Over three-quarters (78%) of the land storms had detectable lightning, while less than half (43%) of the oceanic storms had lightning. We did not find any significant regional or latitudinal based patterns in our total conduction currents. By combining the aircraft derived storm currents and flash rates with diurnal lightning statistics derived from the Lightning Imaging Sensor (LIS) and Optical Transient Detector (OTD) low Earth orbiting satellites, we reproduce the diurnal variation in the global electric circuit (i.e., the Carnegie

  17. A first attempt to derive soil erosion rates from 137Cs airborne gamma measurements in two Alpine valleys

    NASA Astrophysics Data System (ADS)

    Arata, Laura; Meusburger, Katrin; Bucher, Benno; Mabit, Lionel; Alewell, Christine

    2016-04-01

    The application of fallout radionuclides (FRNs) as soil tracers is currently one of the most promising and effective approach for evaluating soil erosion magnitudes in mountainous grasslands. Conventional assessment or measurement methods are laborious and constrained by the topographic and climatic conditions of the Alps. The 137Cs (half-life = 30.2 years) is the most frequently used FRN to study soil redistribution. However the application of 137Cs in alpine grasslands is compromised by the high heterogeneity of the fallout due to the origin of 137Cs fallout in the Alps, which is linked to single rain events occurring just after the Chernobyl accident when most of the Alpine soils were still covered by snow. The aim of this study was to improve our understanding of the 137Cs distribution in two study areas in the Central Swiss Alps: the Ursern valley (Canton Uri), and the Piora valley (Canton Ticino). In June 2015, a helicopter equipped with a NaI gamma detector flew over the two study sites and screened the 137Cs activity of the top soil. The use of airborne gamma measurements is particularly efficient in case of higher 137Cs concentration in the soil. Due to their high altitude and high precipitation rates, the Swiss Alps are expected to be more contaminated by 137Cs fallout than other parts of Switzerland. The airborne gamma measurements have been related to several key parameters which characterize the areas, such as soil properties, slopes, expositions and land uses. The ground truthing of the airborne measurements (i.e. the 137Cs laboratory measurements of the soil samples collected at the same points) returned a good fit. The obtained results offer an overview of the 137Cs concentration in the study areas, which allowed us to identify suitable reference sites, and to analyse the relationship between the 137Cs distribution and the above cited parameters. The authors also derived a preliminary qualitative and a quantitative assessment of soil redistribution

  18. Relationship between RNA/DNA ratio, growth rate and accumulation of selenium in the cells of wheat leaves under the influence of minerals analcime and trepel.

    PubMed

    Martynenko, O I; Kyrylenko, T K; Zaimenko, N V; Antonyuk, M M; Stepanyugin, A V; Plodnik, D P; Hovorun, D M

    2014-01-01

    We studied specific effects of different doses of natural minerals--analcime (An) and trepel (Tr)--on the growth rate, selenium (Se) content and functional activity of the genome of wheat leaves measured by the RNA/DNA ratio. Our results show that under the influence of An and Tr, especially at low doses (25 mg/100 g sand), there is a significant increase in the content of Se, increased growth rate of leaves of wheat seedlings and decreased RNA/DNA ratio. We have found significant correlations between studied parameters. Our findings suggest that the RNA/DNA ratio can be used as a convenient, reliable indicator of the biological activity of minerals An and Tr, and for quantitative express-estimation of their impact on plant organisms.

  19. Validation of heart rate derived from a physiological status monitor-embedded compression shirt against criterion ECG.

    PubMed

    Dolezal, B A; Boland, D M; Carney, J; Abrazado, M; Smith, D L; Cooper, C B

    2014-01-01

    Firefighters are subject to extreme environments and high physical demands when performing duty-related tasks. Recently, physiological status monitors (PSM) have been embedded into a compression shirt to enable firefighters to measure, visualize, log, and transmit vital metrics such as heart rate (HR) to aid in cardiovascular risk identification and mitigation, thereby attempting to improve the health, fitness, and safety of this population. The purpose of this study was to validate HR recorded by the PSM-embedded compression shirt against a criterion standard laboratory ECG-derived HR when worn concurrently with structural firefighting personal protective equipment (PPE) during four simulated firefighting activities. Ten healthy, college-age men (mean ± SD: age: 21 ± 1 yr; body mass: 91 ± 10 kg; body mass index: 26.9 ± 3.1 kg/m(2)) completed four tasks that are routinely performed during firefighting operations: outdoor fast-paced walking (FW), treadmill walking (TW), searching/crawling (SC), and ascending/descending stairs (AD). They wore the PSM-embedded compression shirt under structural firefighting PPE. HR was recorded concurrently by the PSM-embedded compression shirt and a portable metabolic measurement system accompanied with a standard 12-lead electrocardiograph that was used to provide criterion measures of HR. For all four tasks combined there was very high correlation of PSM and ECG HR (r > 0.99; SEE 0.84 /min) with a mean difference (bias) of -0.02 /min and limits of agreement of -0.07 to 0.02 /min. For individual tasks, the correlations were also high (r-values = 0.99; SEE 0.81-0.89). The mean bias (limits of agreement) was: FW 0.03 (-0.09 to 0.14); TW 0.04 (-0.05 to 0.12); SC -0.01 (-0.12 to 0.10); AD -0.13 (-0.21 to -0.04) /min. These findings demonstrate that the PSM-embedded compression shirt provides a valid measure of HR during simulated firefighting activities when compared with a standard 12-lead ECG.

  20. 43 CFR 3211.19 - What is the royalty rate on byproducts derived from geothermal resources produced from or...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 CFR 3211.10(b) (2004). ... derived from geothermal resources produced from or attributable to my lease? 3211.19 Section 3211.19..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE LEASING Filing and Processing...

  1. 43 CFR 3211.19 - What is the royalty rate on byproducts derived from geothermal resources produced from or...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 CFR 3211.10(b) (2004). ... derived from geothermal resources produced from or attributable to my lease? 3211.19 Section 3211.19..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE LEASING Filing and Processing...

  2. 43 CFR 3211.19 - What is the royalty rate on byproducts derived from geothermal resources produced from or...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 CFR 3211.10(b) (2004). ... derived from geothermal resources produced from or attributable to my lease? 3211.19 Section 3211.19..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE LEASING Filing and Processing...

  3. 43 CFR 3211.19 - What is the royalty rate on byproducts derived from geothermal resources produced from or...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 CFR 3211.10(b) (2004). ... derived from geothermal resources produced from or attributable to my lease? 3211.19 Section 3211.19..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE LEASING Filing and Processing...

  4. Frequency and duty cycle modulation optimization in minimizing thermal accumulation effect in Z-scan measurement with high-repetition-rate laser

    NASA Astrophysics Data System (ADS)

    Shahnan Zainal Abidin, Mohd; Noor, Ahmad Shukri Muhammad; Rashid, Suraya Abdul; Adzir Mahdi, Mohd

    2014-11-01

    In this study, we demonstrate the optimization of the chopper frequency and duty cycle in a Z-scan measurement with a 250 MHz high-repetition-rate (HRR) femtosecond laser to minimize the thermal lensing effect due to cumulative heating of the sample. The result shows that such minimization can be achieved by keeping the modulated exposure time on the sample shorter than the thermal diffusivity decay time tc. The minimum chopper frequency fmin is predicted by relating the duty cycle factor F with tc, while maintaining stable peak and valley transmittances, i.e., ΔTp and ΔTv, respectively. Furthermore, a lower fmin is obtained by taking a stable range of the peak-valley difference ΔTpv into consideration. The optimization allows for the low operational modulation frequency of Z-scan measurement with reduced thermal influence, thus enabling simple management of the thermal lensing effect.

  5. Rain rate and modeled fade distributions at 20 GHz and 30 GHz derived from five years of network rain gauge measurements

    NASA Technical Reports Server (NTRS)

    Goldhirsh, Julius; Krichevsky, Vladimir; Gebo, Norman

    1992-01-01

    Five years of rain rate and modeled slant path attenuation distributions at 20 GHz and 30 GHz derived from a network of 10 tipping bucket rain gages was examined. The rain gage network is located within a grid 70 km north-south and 47 km east-west in the Mid-Atlantic coast of the United States in the vicinity of Wallops Island, Virginia. Distributions were derived from the variable integration time data and from one minute averages. It was demonstrated that for realistic fade margins, the variable integration time results are adequate to estimate slant path attenuations at frequencies above 20 GHz using models which require one minute averages. An accurate empirical formula was developed to convert the variable integration time rain rates to one minute averages. Fade distributions at 20 GHz and 30 GHz were derived employing Crane's Global model because it was demonstrated to exhibit excellent accuracy with measured COMSTAR fades at 28.56 GHz.

  6. An accurate derivation of the air dose-rate and the deposition concentration distribution by aerial monitoring in a low level contaminated area

    NASA Astrophysics Data System (ADS)

    Nishizawa, Yukiyasu; Sugita, Takeshi; Sanada, Yukihisa; Torii, Tatsuo

    2015-04-01

    Since 2011, MEXT (Ministry of Education, Culture, Sports, Science and Technology, Japan) have been conducting aerial monitoring to investigate the distribution of radioactive cesium dispersed into the atmosphere after the accident at the Fukushima Dai-ichi Nuclear Power Plant (FDNPP), Tokyo Electric Power Company. Distribution maps of the air dose-rate at 1 m above the ground and the radioactive cesium deposition concentration on the ground are prepared using spectrum obtained by aerial monitoring. The radioactive cesium deposition is derived from its dose rate, which is calculated by excluding the dose rate of the background radiation due to natural radionuclides from the air dose-rate at 1 m above the ground. The first step of the current method of calculating the dose rate due to natural radionuclides is calculate the ratio of the total count rate of areas where no radioactive cesium is detected and the count rate of regions with energy levels of 1,400 keV or higher (BG-Index). Next, calculate the air dose rate of radioactive cesium by multiplying the BG-Index and the integrated count rate of 1,400 keV or higher for the area where the radioactive cesium is distributed. In high dose-rate areas, however, the count rate of the 1,365-keV peak of Cs-134, though small, is included in the integrated count rate of 1,400 keV or higher, which could cause an overestimation of the air dose rate of natural radionuclides. We developed a method for accurately evaluating the distribution maps of natural air dose-rate by excluding the effect of radioactive cesium, even in contaminated areas, and obtained the accurate air dose-rate map attributed the radioactive cesium deposition on the ground. Furthermore, the natural dose-rate distribution throughout Japan has been obtained by this method.

  7. The computerized derivation of rate equations for enzyme reactions on the basis of the pseudo-steady-state assumption and the rapid-equilibrium assumption.

    PubMed Central

    Ishikawa, H; Maeda, T; Hikita, H; Miyatake, K

    1988-01-01

    A computer program is developed for the derivation of the rate equation for enzyme reactions on the basis of the pseudo-steady-state assumption and the combination of the pseudo-steady-state and the rapid-equilibrium assumptions. The program not only has an easy input method, but also can obtain a complete rate equation in itself on only one run. The usefulness of the program is demonstrated by deriving the rate equations for some typical enzyme reactions. Details of the program have been deposited as Supplementary Publication SUP 50141 (42 pages) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7QB, U.K., from whom copies may be obtained as indicated in Biochem. J. (1988), 249, 5. PMID:3390151

  8. Greenland annual accumulation along the EGIG line, 1959-2004, from ASIRAS airborne radar and neutron-probe density measurements

    NASA Astrophysics Data System (ADS)

    Overly, Thomas B.; Hawley, Robert L.; Helm, Veit; Morris, Elizabeth M.; Chaudhary, Rohan N.

    2016-08-01

    We report annual snow accumulation rates from 1959 to 2004 along a 250 km segment of the Expéditions Glaciologiques Internationales au Groenland (EGIG) line across central Greenland using Airborne SAR/Interferometric Radar Altimeter System (ASIRAS) radar layers and high resolution neutron-probe (NP) density profiles. ASIRAS-NP-derived accumulation rates are not statistically different (95 % confidence interval) from in situ EGIG accumulation measurements from 1985 to 2004. ASIRAS-NP-derived accumulation increases by 20 % below 3000 m elevation, and increases by 13 % above 3000 m elevation for the period 1995 to 2004 compared to 1985 to 1994. Three Regional Climate Models (PolarMM5, RACMO2.3, MAR) underestimate snow accumulation below 3000 m by 16-20 % compared to ASIRAS-NP from 1985 to 2004. We test radar-derived accumulation rates sensitivity to density using modeled density profiles in place of NP densities. ASIRAS radar layers combined with Herron and Langway (1980) model density profiles (ASIRAS-HL) produce accumulation rates within 3.5 % of ASIRAS-NP estimates in the dry snow region. We suggest using Herron and Langway (1980) density profiles to calibrate radar layers detected in dry snow regions of ice sheets lacking detailed in situ density measurements, such as those observed by the Operation IceBridge campaign.

  9. Cattle stocking rates estimated in temperate intensive grasslands with a spring growth model derived from MODIS NDVI time-series

    NASA Astrophysics Data System (ADS)

    Green, Stuart; Cawkwell, Fiona; Dwyer, Edward

    2016-10-01

    There is an identified need for high resolution animal stocking rate data in temperate grassland systems. Here is presented a 250 m scale characterization of early spring vegetation growth (DOY 32-DOY 120) from 2003 to 2012 based on MODIS NDVI products for this period for Ireland. The average rate of grass growth is determined locally as a simple linear model for each pixel, using only the highest quality data for the period. These decadal spring growth model coefficients, start of season cover and growth rate, are regressed against log of stocking rate (r2 = 0.75). This model stocking rate is used to map grassland use intensity in Ireland, which, when tested against an independent set of stocking rate data, is shown to be successful with an RMSE error of 0.13 for a range of stocking densities from 0.1 to 3.0 LSU/Ha. This model provides the first validated high resolution approach to mapping stocking rates in intensively managed European grassland systems.

  10. Plant, cell, and molecular mechanisms of abscisic-acid regulation of stomatal apertures. A new mechanism for the regulation of stomatal-aperture size in intact leaves: Accumulation of mesophyll-derived sucrose in the guard-cell wall of Vicia faba L.

    SciTech Connect

    Lu, P.; Outlaw, W.H. Jr.; Smith, B.G.; Freed, G.A.

    1996-12-31

    At various times after pulse labeling Vicia faba L. leaflets with {sup 14}CO{sub 2}, whole-leaf pieces and rinsed epidermal peels were harvested and subsequently processed for histochemical analysis. Cells dissected from whole leaf retained apoplastic contents whereas those from rinsed peels contained only cytoplastic contents. Sucrose specific radioactivity peaked in palisade cells, 111 GBq{center_dot}mol{sup {minus}1}, at 20 min. In contrast, the {sup 14}C content and sucrose specific radioactivity were very low in guard cells for 20 min, implying little CO{sub 2} incorporation; both then peaked at 40 min. The guard-cell apoplast had a high maximum sucrose specific radioactivity and a high sucrose influx rate. These and other comparisons implied the presence of (a) multiple sucrose pools in mesophyll cells, (b) a localized mesophyll-apoplast region that exchanges with phloem and stomata, and (c) mesophyll-derived sucrose in guard-cell walls sufficient to diminish stomatal opening by {approximately} 4 {micro}m. Factors expected to enhance sucrose accumulation in guard-cell walls are (a) high transpiration rate, which closes stomata, and (b) high apoplastic sucrose concentration, which is elevated when mesophyll-sucrose efflux exceeds translocation. Therefore, multiple physiological factors are integrated in the attenuation of stomatal-aperture size by this previously unrecognized mechanism.

  11. Slip rate of the Wadi Araba fault, southern segment of the Dead Sea fault, derived from GPS and geomorphic measurements

    NASA Astrophysics Data System (ADS)

    Klinger, Y.; Lebeon, M.; Amrat, A.; Agnon, A.; Meriaux, A.; Dorbath, L.; Baer, G.; Finkel, R.; Ruegg, J.; Charade, O.; Mayyas, O.; Ryerson, F.; Tapponnier, P.

    2007-12-01

    The Dead Sea Fault (DSF) accommodates the northward displacement of the Arabia plate relative to Sinai. Although it counts among the major strike-slip faults, no agreement has been reached yet about its slip rate, either instantaneous or longer term. Proposed values vary from 2 to 10 mm/yr. We present here an extensive study of the slip rate for the southern segment of DSF, Wadi Araba fault, based on GPS profiles and measurements of offset geomorphologic features, which ages are comprised between 10 and 350 kyr. We installed 17 campaign-style GPS sites distributed along 3 profiles perpendicular to Wadi Araba fault with far-field points up to 90 km away from the fault. The sites have been measured twice, in 1999 and in 2005, during 48h- long sessions. Measurements are complemented by data from permanent GPS stations in Israel. Using a locked fault model, we estimate the slip rate to be 4.9 ± 1.6 mm/yr over 6 years. Tests for possible creep on the fault show that if it exists it is not significant. To estimate the slip rate over longer periods of time, we targeted alluvial fans offset by the fault at 4 sites, that we mapped and sampled for 10Be cosmogenic dating. At one site, preferred offset of 46 ± 4 m of a surface dated at 11 ± 3 kyr yields a slip rate of 4.7 ± 1.9 mm/yr, in very good agreement with the present-day slip rate. At the other sites, preliminary reconstructions show offsets of ~600 m to ~5400 m with ages ranging from 50 to 350 kyr and suggest a preliminary slip rate of 4-9 mm/yr. Ongoing studies aim to better understand the history of the different lobes of the fans, from their emplacement to their abandonment, to reach a tighter constraint on the geomorphologic slip rate. Interestingly, the slip rates presented here, that have been determined along the same transects of the DSF, show good agreement despite differences of methodology (GPS and geomorphology). It suggests that discrepancies between slip rates determined at different time scales could

  12. Analytic expressions for perturbations and partial derivatives of range and range rate of a spacecraft with respect to the coefficient of the second harmonic

    NASA Technical Reports Server (NTRS)

    Georgevic, R. M.

    1973-01-01

    Closed-form analytic expressions for the time variations of instantaneous orbital parameters and of the topocentric range and range rate of a spacecraft moving in the gravitational field of an oblate large body are derived using a first-order variation of parameters technique. In addition, the closed-form analytic expressions for the partial derivatives of the topocentric range and range rate are obtained, with respect to the coefficient of the second harmonic of the potential of the central body (J sub 2). The results are applied to the motion of a point-mass spacecraft moving in the orbit around the equatorially elliptic, oblate sun, with J sub 2 approximately equal to .000027.

  13. Spatial variability of 10Be-derived erosion rates across the southern Peninsular Indian escarpment: A key to landscape evolution across passive margins

    NASA Astrophysics Data System (ADS)

    Mandal, Sanjay Kumar; Lupker, Maarten; Burg, Jean-Pierre; Valla, Pierre G.; Haghipour, Negar; Christl, Marcus

    2015-09-01

    The persistence of significant topography in ancient, tectonically inactive orogenic belts remains one of the outstanding questions in geomorphology. In southern Peninsular India, the impressive topographic relief of the Western Ghat Mountains in tectonic quiescence since at least ca. 65 Ma has raised important questions concerning the long-term mechanism of topographic evolution. Quantifying the distribution of erosion in space and time is critical to understanding landscape evolution. Although the long-term erosion rates are reasonably well known, the short-term erosion rates and the relative importance of factors controlling erosion in southern Peninsular India are less well constrained. We present a new suite of catchment-averaged and local erosion rates using in situ produced 10Be concentrations in river sediments and exposed bedrock samples in southern Peninsular India. Catchment-averaged erosion rates vary from 9.6 ± 0.8 mMa-1 in the highlands to 114.3 ± 13.8 mMa-1 on the escarpment side. Bedrock erosion rates range from 2.4 ± 0.2 mMa-1 in the ridge-top to 143.4 ± 25.4 mMa-1 in active channel beds of the highlands. Catchment-averaged erosion rates derived from the across-escarpment, westward-draining catchments are significantly higher than those derived from the eastward-draining, over highland catchments. The difference indicates that long-term down-wearing of the highland proceeds at lower rates than in the escarpment zones. Catchment-averaged erosion rates are moderately correlated with mean hillslope angles and local relief whereas they are strongly correlated with catchment-averaged channel steepness index. This suggests that topographic steepness is the major control on the spatial variability of erosion while strong rainfall gradient is of minor importance in this area. 10Be-derived average erosion rates in highlands are consistent with previous long-term erosion rate estimated from thermochronometry. These results collectively point to large

  14. Intertemporal choice as discounted value accumulation.

    PubMed

    Rodriguez, Christian A; Turner, Brandon M; McClure, Samuel M

    2014-01-01

    Two separate cognitive processes are involved in choosing between rewards available at different points in time. The first is temporal discounting, which consists of combining information about the size and delay of prospective rewards to represent subjective values. The second involves a comparison of available rewards to enable an eventual choice on the basis of these subjective values. While several mathematical models of temporal discounting have been developed, the reward selection process has been largely unexplored. To address this limitation, we evaluated the applicability of the Linear Ballistic Accumulator (LBA) model as a theory of the selection process in intertemporal choice. The LBA model formalizes the selection process as a sequential sampling algorithm in which information about different choice options is integrated until a decision criterion is reached. We compared several versions of the LBA model to demonstrate that choice outcomes and response times in intertemporal choice are well captured by the LBA process. The relationship between choice outcomes and response times that derives from the LBA model cannot be explained by temporal discounting alone. Moreover, the drift rates that drive evidence accumulation in the best-fitting LBA model are related to independently estimated subjective values derived from various temporal discounting models. These findings provide a quantitative framework for predicting dynamics of choice-related activity during the reward selection process in intertemporal choice and link intertemporal choice to other classes of decisions in which the LBA model has been applied.

  15. Bedload transport rates in a gravel bedded-river derived from high-resolution monitoring using seismic impact plates

    NASA Astrophysics Data System (ADS)

    Downs, Peter; Soar, Philip

    2015-04-01

    Accurate characterisation of bedload transport rates is critical for a better understanding of geomorphological process dynamics, aquatic habitats, sediment budgets and strategies for catchment-scale initiatives in sediment management under conditions of climate change. However, rate estimation is challenging in practice: direct measurements are costly and logistically difficult to achieve with acceptable accuracy over geomorphologically-relevant time periods, and the uncertainty in transport rates predicted from empirical formulae and numerical simulation is rarely below 50 per cent. Partly reflecting these issues, passive technologies for continuous bedload monitoring are becoming increasingly popular. Sensors such as seismic impact plates offer the opportunity to characterise bedload activity at exceptionally high resolution - monitoring from the River Avon, (Devon, UK) indicated that despite significant intra-event and between-plate differences in apparent bedload transport aggregated over 5-minute periods, the magnitude-frequency product of discharge and impact frequency result in a highly plausible effective discharge, supporting the potential value of impact plates as indicators of relative sediment transport loads over annual timescales. Whereas the focus in bedload rate estimation to date has been on developing satisfactory sediment rating curves from detection signals, we instead develop a method for directly estimating bedload transport rates from impact plate data as a function of intensity of transport (count, n, per second), bed material mass (kg) and cross-stream transport variability. Bulk sediment samples are converted to a mass in transit for each instantaneous discharge according to the intensity of transport and a Monte Carlo simulation of the load in transit determined at random from the bed material particle size distribution. The lower detection threshold is determined using experimental calibration and the upper size limit is determined from

  16. Peak Satellite-to-Earth Data Rates Derived From Measurements of a 20 Gbps Bread-Board Modem

    NASA Technical Reports Server (NTRS)

    Landon, David G.; Simons, Rainee N.; Wintucky, Edwin G.; Sun, Jun Y.; Winn, James S.; Laraway, Stephen A.; McIntire, William K.; Metz, John L.; Smith, Francis J.

    2011-01-01

    A prototype data link using a Ka-band space qualified, high efficiency 200 W TWT amplifier and a bread-board modem emulator were created to explore the feasibility of very high speed communications in satellite-to-earth applications. Experiments were conducted using a DVB-S2-like waveform with modifications to support up to 20 Gbps through the addition of 128-Quadrature Amplitude Modulation (QAM). Limited by the bandwidth of the amplifier, a constant peak symbol rate of 3.2 Giga-symbols/sec was selected and the modulation order was varied to explore what peak data rate might be supported by an RF link through this amplifier. Using 128-QAM, an implementation loss of 3 dB was observed at 20 Gbps, and the loss decreased as data rate or bandwidth were reduced. Building on this measured data, realistic link budget calculations were completed. Low-Earth orbit (LEO) missions based on this TWTA with reasonable hardware assumptions and antenna sizing are found to be bandwidth-limited, rather than power-limited, making the spectral efficiency of 9/10-rate encoded 128-QAM very attractive. Assuming a bandwidth allocation of 1 GHz, these computations indicate that low-Earth orbit vehicles could achieve data rates up to 5 Gbps-an order of magnitude beyond the current state-of-practice, yet still within the processing power of a current FPGA-based software-defined modem. The measured performance results and a description of the experimental setup are presented to support these conclusions.

  17. Sexual selection and maintenance of sex: evidence from comparisons of rates of genomic accumulation of mutations and divergence of sex-related genes in sexual and hermaphroditic species of Caenorhabditis.

    PubMed

    Artieri, Carlo G; Haerty, Wilfried; Gupta, Bhagwati P; Singh, Rama S

    2008-05-01

    Several hypotheses have been proposed to explain the persistence of dioecy despite the reproductive advantages conferred to hermaphrodites, including greater efficiency at purging deleterious mutations in the former. Dioecy can benefit from both mutation purging and accelerated evolution by bringing together beneficial mutations in the same individual via recombination and shuffling of genotypes. In addition, mathematical treatment has shown that sexual selection is also capable of mitigating the cost of maintaining separate sexes by increasing the overall fitness of sexual populations, and genomic comparisons have shown that sexual selection can lead to accelerated evolution. Here, we examine the advantages of dioecy versus hermaphroditism by comparing the rate of evolution in sex-related genes and the rate of accumulation of deleterious mutations using a large number of orthologs (11,493) in the dioecious Caenorhabditis remanei and the hermaphroditic Caenorhabditis briggsae. We have used this data set to estimate the deleterious mutation rate per generation, U, in both species and find that although it is significantly higher in hermaphrodites, both species are at least 2 orders of magnitude lower than the value required to explain the persistence of sex by efficiency at purging deleterious mutations alone. We also find that genes expressed in sperm are evolving rapidly in both species; however, they show a greater increase in their rate of evolution relative to genes expressed in other tissues in C. remanei, suggesting stronger sexual selection pressure acting on these genes in dioecious species. Interestingly, the persistence of a signal of rapid evolution of sperm genes in C. briggsae suggests a recent evolutionary origin of hermaphrodism in this lineage. Our results provide empirical evidence of increased sexual selection pressure in dioecious animals, supporting the possibility that sexual selection may play an important role in the maintenance of sexual

  18. 10Be-derived denudation rates from the Burdekin catchment: The largest contributor of sediment to the Great Barrier Reef

    NASA Astrophysics Data System (ADS)

    Croke, Jacky; Bartley, Rebecca; Chappell, John; Austin, Jenet M.; Fifield, Keith; Tims, Stephen G.; Thompson, Chris J.; Furuichi, Takahisa

    2015-07-01

    Terrestrial cosmogenic nuclides (TCNs) such as Beryllium-10 (10Be) are now routinely used to reconstruct erosional rates over tens of thousands of years at increasingly large basin scales (> 100,000 km2). In Australia, however, the approach and its assumptions have not been systematically tested within a single, large drainage basin. This study measures 10Be concentrations in river sediments from the Burdekin catchment, one of Australia's largest coastal catchments, to determine long-term (> 10,000 years), time-integrated rates of sediment generation and denudation. A nested-sampling design was used to test for effects of increasing catchment scale on nuclide concentrations with upstream catchment areas ranging from 4 to 130,000 km2. Beryllium-10 concentrations in sediment samples collected from the upstream headwater tributaries and mid-stream locations range from 1.8 to 2.89 × 105 atoms g- 1 and data confirm that nuclide concentrations are well and rapidly mixed downstream. Sediment from the same tributaries consistently yielded 10Be concentrations in the range of their upstream samples. Overall, no decrease in 10Be concentrations can be observed at the range of catchment scales measured here. The mean denudation rate for all river sediment samples throughout the Fanning subcatchment (1100 km2) is 18.47 m Ma- 1, which compares with the estimate at the end of the Burdekin catchment (130,000 km2) of 16.22 m Ma- 1. Nuclide concentrations in the lower gradient western and southern catchments show a higher degree of variability, and several complications emerged as a result of the contrasting geomorphic processes and settings. This study confirms the ability of TCNs to determine long-term denudation rates in Australia and highlights some important considerations in the model assumptions that may affect the accuracy of limited sampling in large, low-gradient catchments with long storage times.

  19. Nutrient-contaminant (Pu) plant accumulation model

    SciTech Connect

    Cowan, C.E.; Jenne, E.A.; Simpson, J.C.; Cataldo, D.A.

    1981-12-01

    A model was developed which simulates the movement and daily accumulation of nutrients and contaminants in crop plants resulting from known physiological processes in the plant. In the model, the daily contaminant accumulation is governed by daily increase in plant biomass derived from photosynthesis and by the specified thermodynamic activity of the bioavailable contaminant species in soil or hydroponic solutin. Total accumulation and resulting concentration in the plant's root, stem and branch, leaf, and reproductive compartments can be simulated any time during the growing season. Parameters were estimated from data on plutonium accumulation in soybeans and the model was calibrated against this same data set. The plutonium distribution in the plant was found to be most sensitive to parameters related to leaf accumulation. Contamination at different times during the growing season resulted in a large change in predicted leaf accumulation but very little change in predicted accumulation in other plant parts except when contamination occurred very late in the growing season.

  20. Spatial and temporal variability of snow accumulation in East Antarctica from traverse data

    NASA Astrophysics Data System (ADS)

    Frezzotti, Massimo; Pourchet, Michel; Flora, Onelio; Gandolfi, Stefano; Gay, Michel; Urbini, Stefano; Vincent, Christian; Becagli, Silvia; Gragnani, Roberto; Proposito, Marco; Severi, Mirko; Traversi, Rita; Udisti, Roberto; Fily, Michel

    Recent snow accumulation rate is a key quantity for ice-core and mass-balance studies. Several accumulation measurement methods (stake farm, fin core, snow-radar profiling, surface morphology, remote sensing) were used, compared and integrated at eight sites along a transect from Terra Nova Bay to Dome C, East Antarctica, to provide information about the spatial and temporal variability of snow accumulation. Thirty-nine cores were dated by identifying tritium/β marker levels (1965-66) and non-sea-salt (nss) SO42- spikes of the Tambora (Indonesia) volcanic event (1816) in order to provide information on temporal variability. Cores were linked by snow radar and global positioning system surveys to provide detailed information on spatial variability in snow accumulation. Stake-farm and ice-core accumulation rates are observed to differ significantly, but isochrones (snow radar) correlate well with ice-core derived accumulation. The accumulation/ablation pattern from stake measurements suggests that the annual local noise (metre scale) in snow accumulation can approach 2 years of ablation and more than four times the average annual accumulation, with no accumulation or ablation for a 5 year period in up to 40% of cases. The spatial variability of snow accumulation at the kilometre scale is one order of magnitude higher than temporal variability at the multi-decadal/secular scale. Stake measurements and firn cores at Dome C confirm an approximate 30% increase in accumulation over the last two centuries, with respect to the average over the last 5000 years.

  1. Present-day kinematics and fault slip rates in eastern Iran, derived from 11 years of GPS data

    NASA Astrophysics Data System (ADS)

    Walpersdorf, A.; Manighetti, I.; Mousavi, Z.; Tavakoli, F.; Vergnolle, M.; Jadidi, A.; Hatzfeld, D.; Aghamohammadi, A.; Bigot, A.; Djamour, Y.; Nankali, H.; Sedighi, M.

    2014-02-01

    We analyze new GPS data spanning 11 years at 92 stations in eastern Iran. We use these data to analyze the present-day kinematics and the slip rates on most seismogenic faults in eastern Iran. The east Lut, west Lut, Kuhbanan, Anar, Dehshir, and Doruneh faults are confirmed as the major faults and are found to currently slip laterally at 5.6 ± 0.6, 4.4 ± 0.4, 3.6 ± 1.3, 2.0 ± 0.7, 1.4 ± 0.9, and 1.3 ± 0.8 mm/yr, respectively. Slip is right-lateral on the ~NS striking east Lut, west Lut, Kuhbanan, Anar, and Dehshir faults and left-lateral on the ~EW Doruneh fault. The ~NS faults slice the eastern Iranian crust into five blocks that are moving northward at 6-13 mm/yr with respect to the stable Afghan crust at the eastern edge of the collision zone. The collective behavior of the ~NS faults might thus allow the Arabian promontory to impinge northward into the Eurasian crust. The ~NS faults achieve additional NS shortening by rotating counterclockwise in the horizontal plane, at current rates up to 0.8°/Ma. Modeling the GPS and available geological data with a block rotation model suggests that the rotations have been going on at a similar rate (1 ± 0.4°/Ma) over the last 12 Ma. We identify large strains at the tips of the rotating east Lut, west Lut, and Kuhbanan faults, which we suspect to be responsible for the important historical and instrumental seismicity in those zones.

  2. Derivation of cardiac output and alveolar ventilation rate based on energy expenditure measurements in healthy males and females.

    PubMed

    Brochu, Pierre; Brodeur, Jules; Krishnan, Kannan

    2012-08-01

    Physiologically based pharmacokinetic modeling and occupational exposure assessment studies often use minute ventilation rates (VE), alveolar ventilation rates (VA) and cardiac outputs (Q) that are not reflective of the physiological variations encountered during the aggregate daytime activities of individuals from childhood to adulthood. These variations of VE, VA and Q values were determined for healthy normal-weight individuals aged 5-96 years by using two types of published individual data that were measured in the same subjects (n = 902), namely indirect calorimetry measurements and the disappearance rates of oral doses of deuterium (²H) and heavy-oxygen (¹⁸O) in urine monitored by gas-isotope-ratio mass spectrometry. Arteriovenous oxygen content differences (0.051-0.082 ml of O₂ consumed ml⁻¹ of blood) and ratios of the physiological dead space to the tidal volume (0.232-0.419) were determined for oxygen consumption rates (0.157-0.806 l min⁻¹) required by minute energy expenditures ranging from 0.76 to 3.91 kcal min⁻¹. Generally higher values for the 2.5th up to the 99th percentile for VE (0.132-0.774 l kg⁻¹ min⁻¹, 4.42-21.69 l m⁻² min⁻¹), VA (0.093-0.553 l kg⁻¹ min⁻¹, 3.09-15.53 l m⁻² min⁻¹), Q (0.065-0.330 l kg⁻¹ min⁻¹, 2.17 to 9.46 l m⁻² min⁻¹) and ventilation-perfusion ratios (1.12-2.16) were found in children and teenagers aged 5-<16.5 years compared with older individuals. The distributions of cardiopulmonary parameters developed in this study should be useful in facilitating a scientifically sound characterization of the inter-individual differences in the uptake and health risks of lipophilic air pollutants, particularly as they relate to younger children.

  3. Derivation of a formula describing the saturation correction of plane-parallel ionization chambers in pulsed fields with arbitrary repetition rate

    NASA Astrophysics Data System (ADS)

    Karsch, Leonhard

    2016-04-01

    Gas-filled ionization chambers are widely used radiation detectors in radiotherapy. A quantitative description and correction of the recombination effects exists for two cases, for continuous radiation exposure and for pulsed radiation fields with short single pulses. This work gives a derivation of a formula for pulsed beams with arbitrary pulse rate for which the prerequisites of the two existing descriptions are not fulfilled. Furthermore, an extension of the validity of the two known cases is investigated. The temporal evolution of idealized charge density distributions within a plane parallel chamber volume is described for pulsed beams of vanishing pulse duration and arbitrary pulse repetition rate. First, the radiation induced release, movement and collection of the charge carriers without recombination are considered. Then, charge recombination is calculated basing on these simplified charge distributions and the time dependent spatial overlap of positive and negative charge carrier distributions. Finally, a formula for the calculation of the saturation correction factor is derived by calculation and simplification of the first two terms of a Taylor expansion for small recombination. The new formula of saturation correction contains the two existing cases, descriptions for exposure by single pulses and continuous irradiation, as limiting cases. Furthermore, it is possible to determine the pulse rate range for which each of the three descriptions is applicable by comparing the dependencies of the new formula with the two existing cases. As long as the time between two pulses is lower than one third of the collection time of the chamber, the formalism for a continuous exposure can be used. The known description for single pulse irradiation is only valid if the repetition rate is less than 1.2 times the inverse collection time. For all other repetition rates in between the new formula should be used. The experimental determination by Jaffe diagrams can be

  4. Mesoscale distributions of ultraviolet spectral irradiance, actinic flux, and photolysis rates derived from multispectral satellite data and radiative transfer models

    NASA Astrophysics Data System (ADS)

    Wetzel, Melanie A.; Slusser, James R.

    2005-04-01

    Global atmospheric trends in ozone column amount have focused attention on the environmental risk of exposure to ultraviolet (UV) radiation. Monitoring UV irradiance in diverse and remote locations is necessary to understand the variability of exposure, dose rates, and resultant vulnerability of ecological systems. The U.S. Department of Agriculture (USDA) UV-B Monitoring Program maintains a wide network for ground-based continuous measurement of solar radiation in several wavelengths of interest for photosynthesis, plant growth, UV exposure to humans, and photochemistry. This network provides data for analysis of UV climatology and trends at those sites. A satellite-based method to produce mesoscale-resolution mapped distributions of UV spectral irradiance has been developed that utilizes this network data for verification. The methodology combines radiative transfer modeling, multispectral image pixel classification, cloud optical depth retrievals, and auxiliary remote sensing data. Retrieved parameters are compared with ground-based measurements from the USDA network instrumentation at two sites [Poker Flat Research Range, Alaska; 65.12 deg N, 147.43 deg W, 550 m mean sea level (MSL); and Storm Peak Laboratory, Colorado, 40.45 deg N, 106.73 deg W, 3220 m MSL]. The results are used to evaluate the effects of cloud distribution and surface albedo in determining mesoscale variability of UV exposure and photolysis rates in high-latitude and high-altitude environments.

  5. Field metabolic rate and PCB adipose tissue deposition efficiency in East Greenland polar bears derived from contaminant monitoring data.

    PubMed

    Pavlova, Viola; Nabe-Nielsen, Jacob; Dietz, Rune; Svenning, Jens-Christian; Vorkamp, Katrin; Rigét, Frank Farsø; Sonne, Christian; Letcher, Robert J; Grimm, Volker

    2014-01-01

    Climate change will increasingly affect the natural habitat and diet of polar bears (Ursus maritimus). Understanding the energetic needs of polar bears is therefore important. We developed a theoretical method for estimating polar bear food consumption based on using the highly recalcitrant polychlorinated biphenyl (PCB) congener, 2,2',4,4',55-hexaCB (CB153) in bear adipose tissue as an indicator of food intake. By comparing the CB153 tissue concentrations in wild polar bears with estimates from a purposely designed individual-based model, we identified the possible combinations of field metabolic rates (FMR) and CB153 deposition efficiencies in East Greenland polar bears. Our simulations indicate that if 30% of the CB153 consumed by polar bear individuals were deposited into their adipose tissue, the corresponding FMR would be only two times the basal metabolic rate. In contrast, if the modelled CB153 deposition efficiency were 10%, adult polar bears would require six times more energy than that needed to cover basal metabolism. This is considerably higher than what has been assumed for polar bears in previous studies though it is similar to FMRs found in other marine mammals. An implication of this result is that even relatively small reductions in future feeding opportunities could impact the survival of East Greenland polar bears. PMID:25101837

  6. Theoretical derivation for reaction rate constants of H abstraction from thiophenol by the H/O radical pool

    PubMed Central

    Batiha, Marwan; Altarawneh, Mohammednoor; Al-Harahsheh, Mohammad; Altarawneh, Ibrahem; Rawadieh, Saleh

    2011-01-01

    Reaction and activation energy barriers are calculated for the H abstraction reactions (C6H5SH + X• → C6H5S + XH, X = H, OH and HO2) at the BB1K/GTLarge level of theory. The corresponding reactions with H2S and CH3SH are also investigated using the G3B3 and CBS-QB3 methods in order to demonstrate the accuracy of BB1K functional in finding activation barriers for hydrogen atom transfer reactions. Arrhenius parameters for the title reactions are fitted in the temperature range of 300 K–2000 K. The calculated reaction enthalpies are in good agreement with their corresponding experimental reaction enthalpies. It is found that H abstraction by OH radicals from the thiophenol molecule proceed in a much slower rate in reference to the analogous phenol molecule. ΔfH298o of thiophenoxy radical is calculated to be 63.3 kcal/mol. Kinetic parameters presented herein should be useful in describing the decomposition rate of thiophenol; i.e., one of the major aromatic sulfur carriers, at high temperatures. PMID:22485200

  7. Field metabolic rate and PCB adipose tissue deposition efficiency in East Greenland polar bears derived from contaminant monitoring data.

    PubMed

    Pavlova, Viola; Nabe-Nielsen, Jacob; Dietz, Rune; Svenning, Jens-Christian; Vorkamp, Katrin; Rigét, Frank Farsø; Sonne, Christian; Letcher, Robert J; Grimm, Volker

    2014-01-01

    Climate change will increasingly affect the natural habitat and diet of polar bears (Ursus maritimus). Understanding the energetic needs of polar bears is therefore important. We developed a theoretical method for estimating polar bear food consumption based on using the highly recalcitrant polychlorinated biphenyl (PCB) congener, 2,2',4,4',55-hexaCB (CB153) in bear adipose tissue as an indicator of food intake. By comparing the CB153 tissue concentrations in wild polar bears with estimates from a purposely designed individual-based model, we identified the possible combinations of field metabolic rates (FMR) and CB153 deposition efficiencies in East Greenland polar bears. Our simulations indicate that if 30% of the CB153 consumed by polar bear individuals were deposited into their adipose tissue, the corresponding FMR would be only two times the basal metabolic rate. In contrast, if the modelled CB153 deposition efficiency were 10%, adult polar bears would require six times more energy than that needed to cover basal metabolism. This is considerably higher than what has been assumed for polar bears in previous studies though it is similar to FMRs found in other marine mammals. An implication of this result is that even relatively small reductions in future feeding opportunities could impact the survival of East Greenland polar bears.

  8. Field Metabolic Rate and PCB Adipose Tissue Deposition Efficiency in East Greenland Polar Bears Derived from Contaminant Monitoring Data

    PubMed Central

    Pavlova, Viola; Nabe-Nielsen, Jacob; Dietz, Rune; Svenning, Jens-Christian; Vorkamp, Katrin; Rigét, Frank Farsø; Sonne, Christian; Letcher, Robert J.; Grimm, Volker

    2014-01-01

    Climate change will increasingly affect the natural habitat and diet of polar bears (Ursus maritimus). Understanding the energetic needs of polar bears is therefore important. We developed a theoretical method for estimating polar bear food consumption based on using the highly recalcitrant polychlorinated biphenyl (PCB) congener, 2,2′,4,4′,55-hexaCB (CB153) in bear adipose tissue as an indicator of food intake. By comparing the CB153 tissue concentrations in wild polar bears with estimates from a purposely designed individual-based model, we identified the possible combinations of field metabolic rates (FMR) and CB153 deposition efficiencies in East Greenland polar bears. Our simulations indicate that if 30% of the CB153 consumed by polar bear individuals were deposited into their adipose tissue, the corresponding FMR would be only two times the basal metabolic rate. In contrast, if the modelled CB153 deposition efficiency were 10%, adult polar bears would require six times more energy than that needed to cover basal metabolism. This is considerably higher than what has been assumed for polar bears in previous studies though it is similar to FMRs found in other marine mammals. An implication of this result is that even relatively small reductions in future feeding opportunities could impact the survival of East Greenland polar bears. PMID:25101837

  9. High-etch-rate bottom-antireflective coating and gap-fill materials using dextrin derivatives in via first dual-Damascene lithography process

    NASA Astrophysics Data System (ADS)

    Takei, Satoshi; Sakaida, Yasushi; Shinjo, Tetsuya; Hashimoto, Keisuke; Nakajima, Yasuyuki

    2008-03-01

    The present paper describes a novel class of bottom antireflective coating (BARC) and gap fill materials using dextrin derivatives. The general trend of interconnect fabrication for such a high performance LSI is to apply cupper (Cu)/ low-dielectric-constant (low-k) interconnect to reduce RC delay. A via-first dual damascene process is one of the most promising processes to fabricate Cu/ low-k interconnect due to its wide miss-alignment margin. The sacrificial materials containing dextrin derivatives under resist for lithography were developed in via-first dual damascene process. The dextrin derivatives in this study was obtained by the esterification of the hydroxyl groups of dextrin resulting in improved solubility in the resist solvents such as propylene glycol monomethylether, propylene glycol monomethylether acetate, and ethyl lactate due to avoid the issue of defects that were caused by incompatability. The etch rate of our developed BARC and gap fill materials using dextrin derivatives was more than two times faster than one of the ArF resists evaluated in a CF4 gas condition using reactive ion etching. The improved etch performance was also verified by comparison with poly(hydroxystyrene), acrylate-type materials and latest low-k materials as a reference. In addition to superior etch performance, these materials showed good resist profiles and via filling performance without voids in via holes.

  10. Influence of soil pH and application rate on the oxidation of calcium sulfite derived from flue gas desulfurization

    SciTech Connect

    Lee, Y.B.; Bigham, J.M.; Dick, W.A.; Jones, E.S.; Ramsier, C.

    2007-01-15

    Calcium sulfite hemihydrate (CaSO{sub 3} {center_dot} 0.5H{sub 2}O), a common byproduct of coal-fired utilities, is fairly insoluble and can decompose to release toxic SO{sub 2} under highly acidic soil conditions; however, it can also oxidize to form gypsum. The objective of this study was to examine the effects of application rate and soil pH on the oxidation of calcium sulfite under laboratory conditions. Oxidation rates measured by release of SO{sub 4}-S to solution decreased with increasing application rate. Leachate SO{sub 4}-S from soils amended with 1.0 to 3.0 g kg{sup -1} CaSO{sub 3} increased over a 21 to 28 d period before reaching a plateau. At 4 g kg{sup -1}, maximum SO{sub 4}-S release was delayed until Week 7. Oxidation and release of SO{sub 4}-S from soil amended with 3.0 g kg{sup -1} calcium sulfite increased markedly with decreasing soil pH. After only 3 d incubation, the concentrations of SO{sub 4}-S in aqueous leachates were 77, 122, 1709 220, and 229 mg L{sup -1} for initial soil pH values of 7.8, 6.5, 5.5, 5.1, and 4.0, respectively. At an initial soil pH value of 4.0, oxidation/dissolution did not increase much after 3 d. At higher pH values, oxidation was maximized after 21 d. These results suggest that autumn surface applications of calcium sulfite in no-till systems should permit ample time for oxidation/dissolution reactions to occur without introducing biocidal effects related to oxygen scavenging. Soil and annual crops can thus benefit from additions of soluble Ca and SO{sub 4} if calcium sulfite is applied in advance of spring planting.

  11. Organic-Carbon Sequestration in Soil/Sediment of the Mississippi River Deltaic Plain - Data; Landscape Distribution, Storage, and Inventory; Accumulation Rates; and Recent Loss, Including a Post-Katrina Preliminary Analysis (Chapter B)

    USGS Publications Warehouse

    Markewich, Helaine W.; Buell, Gary R.; Britsch, Louis D.; McGeehin, John P.; Robbins, John A.; Wrenn, John H.; Dillon, Douglas L.; Fries, Terry L.; Morehead, Nancy R.

    2007-01-01

    Soil/sediment of the Mississippi River deltaic plain (MRDP) in southeastern Louisiana is rich in organic carbon (OC). The MRDP contains about 2 percent of all OC in the surface meter of soil/sediment in the Mississippi River Basin (MRB). Environments within the MRDP differ in soil/sediment organic carbon (SOC) accumulation rate, storage, and inventory. The focus of this study was twofold: (1) develop a database for OC and bulk density for MRDP soil/sediment; and (2) estimate SOC storage, inventory, and accumulation rates for the dominant environments (brackish, intermediate, and fresh marsh; natural levee; distributary; backswamp; and swamp) in the MRDP. Comparative studies were conducted to determine which field and laboratory methods result in the most accurate and reproducible bulk-density values for each marsh environment. Sampling methods included push-core, vibracore, peat borer, and Hargis1 sampler. Bulk-density data for cores taken by the 'short push-core method' proved to be more internally consistent than data for samples collected by other methods. Laboratory methods to estimate OC concentration and inorganic-constituent concentration included mass spectrometry, coulometry, and loss-on-ignition. For the sampled MRDP environments, these methods were comparable. SOC storage was calculated for each core with adequate OC and bulk-density data. SOC inventory was calculated using core-specific data from this study and available published and unpublished pedon data linked to SSURGO2 map units. Sample age was estimated using isotopic cesium (137Cs), lead (210Pb), and carbon (14C), elemental Pb, palynomorphs, other stratigraphic markers, and written history. SOC accumulation rates were estimated for each core with adequate age data. Cesium-137 profiles for marsh soil/sediment are the least ambiguous. Levee and distributary 137Cs profiles show the effects of intermittent allochthonous input and/or sediment resuspension. Cesium-137 and 210Pb data gave the most

  12. Laboratory-derived temperature preference and effect on the feeding rate and survival of juvenile Hemimysis anomala

    USGS Publications Warehouse

    Sun, Jennifer; Rudstam, Lars S.; Boscarino, Brent T.; Walsh, Maureen G.; Lantry, Brian F.

    2013-01-01

    Hemimysis anomala is a warm-water mysid that invaded the Great Lakes region in 2006 and has since rapidly spread throughout the basin. We conducted three laboratory experiments to better define the temperature preference, tolerance limits, and temperature effects on feeding rates of juvenile Hemimysis, using individuals acclimated to mid (16 °C) and upper (22 °C) preferred temperature values previously reported for the species. For temperature preference, we fit a two-parameter Gaussian (μ, σ) function to the experimental data, and found that the peak values (μ, interpreted as the preference temperature) were 22.0 °C (SE 0.25) when acclimated to 16 and 21.9 °C (SE 0.38) when acclimated to 22 °C, with the σ-values of the curves at 2.6 and 2.5 °C, respectively. No mysids were observed in temperatures below 10 or above 28 °C in these preference experiments. In short-term tolerance experiments for temperatures between 4 and 32 °C, all mysids died within 8 h at 30.2 °C for 16 °C acclimated mysids, and at 31.8 °C for 22 °C acclimated mysids. No lower lethal limit was found. Feeding rates increased with temperature from an average of 4 Bosmina eaten per hour at 5 °C to 19 Bosmina eaten per hour at 27 °C. The results of our experiments indicate an optimal temperature for Hemimysis between 21 and 27 °C, which corresponds with temperatures during periods of high population growth in the field. These results contribute a better understanding of this species' biological response to temperature that will help guide field studies and inform bioenergetics modeling.

  13. Cysteamine supplementation during in vitro maturation of slaughterhouse- and opu-derived bovine oocytes improves embryonic development without affecting cryotolerance, pregnancy rate, and calf characteristics.

    PubMed

    Merton, J S; Knijn, H M; Flapper, H; Dotinga, F; Roelen, B A J; Vos, P L A M; Mullaart, E

    2013-09-01

    Optimization of ovum pick up (OPU) followed by in vitro embryo production (IVP) is strongly driven by the needs of both beef and dairy cattle breeders to enhance genetic improvement. The rapidly growing use of genomic selection in cattle has increased the interest in using OPU-IVP technology to increase the number of embryos and offspring per donor, thus allowing enhanced selection intensity for the next generation. The aim of this study was to optimize embryo production through supplementation of cysteamine during in vitro maturation (IVM) and in vitro culture (IVC) of both slaughterhouse- and OPU-derived oocytes. The effects on embryo production and on embryo cryotolerance, post-transfer embryo survival, and calf characteristics, including gestation length, birth weight, perinatal mortality, and sex ratio were studied. In study 1, immature slaughterhouse-derived cumulus-oocyte complexes (COCs) were matured in IVM medium supplemented with or without 0.1 mM cysteamine, fertilized and cultured for 7 days in 0.5 ml SOFaaBSA. In study 2, cysteamine was present during both IVM (0.1 mM) and IVC (0.01, 0.05, 0.1 mM) from Days 1 to 4. In study 3, OPU-derived COCs were matured in medium supplemented with or without 0.1 mM cysteamine in a 2 × 2 factorial design (OPU week and cysteamine treatment). Embryos were evaluated for stage and grade on Day 7 and, depending on the number of transferable embryos and recipients available, the embryos were transferred either fresh or frozen-thawed at a later date. The presence of cysteamine during IVM significantly increased the embryo production rate with slaughterhouse-derived COCs (24.0% vs. 19.4%). The higher number of embryos at Day 7 was due to an increased number of blastocysts, whereas the distribution of embryos among different quality grades and cryotolerance was not affected. Embryo production rate was negatively affected when cysteamine was present during both the processes of IVM and IVC during Days 1 to 4 of culture (13

  14. Sugar Accumulation in Sugarcane

    PubMed Central

    Gayler, K. R.; Glasziou, K. T.

    1972-01-01

    The rate-limiting reaction for glucose uptake in storage tissue of sugarcane, Saccharum officinarum L., appears to be the movement of glucose across the boundary between the free space and the metabolic compartments. The mechanism for uptake of glucose across this boundary has been studied using 3-O-methyl glucose, an analogue of glucose which is not metabolized by sugar-cane tissue. This analogue is taken up by sugarcane storage tissue at a similar rate to glucose. Its rate of uptake follows Michaelis-Menten kinetics, Km = 1.9 mm, and it is competitively inhibited by glucose, Ki = 2 to 3 mm. Glucose uptake is similarly inhibited by 3-O-methyl glucose. Uptake of 3-O-methyl glucose is energy-dependent and does not appear to be the result of counterflow of glucose. It is concluded that glucose and 3-O-methyl glucose uptake across the boundary between the free space and the metabolic compartment in this tissue is mediated by an energy-dependent carrier system capable of accumulating the sugars against a concentration gradient. PMID:16658002

  15. Ethambutol-mediated cell wall modification in recombinant Corynebacterium glutamicum increases the biotransformation rates of cyclohexanone derivatives.

    PubMed

    Yun, Ji-Yeong; Lee, Jung-Eun; Yang, Kyung-Mi; Cho, Suekyung; Kim, Arim; Kwon, Yong-Uk; Kwon, Yong-Euk; Park, Jin-Byung

    2012-01-01

    The effects of structural modification of cell wall on the biotransformation capability by recombinant Corynebacterium glutamicum cells, expressing the chnB gene encoding cyclohexanone monooxygenase of Acinetobacter calcoaceticus NCIMB 9871, were investigated. Baeyer-Villiger oxygenation of 2-(2'-acetoxyethyl) cyclohexanone (MW 170 Da) into R-7-(2'-acetoxyethyl)-2-oxepanone was used as a model reaction. The whole-cell biotransformation followed Michaelis-Menten kinetics. The V (max) and K (S) values were estimated as 96.8 U g(-1) of dry cells and 0.98 mM, respectively. The V (max) was comparable with that of cyclohexanone oxygenation, whereas the K (S) was almost eightfold higher. The K (S) value of 2-(2'-acetoxyethyl) cyclohexanone oxygenation was reduced by ca. 30% via altering the cell envelop structure of C. glutamicum with ethambutol, which inhibits arabinosyl transferases involved in the biosynthesis of cell wall arabinogalactan and mycolate layers. The higher whole-cell biotransformation rate was also observed in the oxygenation of ethyl 2-cyclohexanone acetate upon ethambutol treatment of the recombinant C. glutamicum. Therefore, it was assumed that the biotransformation efficiency of C. glutamicum-based biocatalysts, with respect to medium- to large-sized lipophilic organic substrates (MW > ca. 170), can be enhanced by engineering their cell wall outer layers, which are known to function as a formidable barrier to lipophilic molecules.

  16. Mean-field equations for stochastic firing-rate neural fields with delays: Derivation and noise-induced transitions

    NASA Astrophysics Data System (ADS)

    Touboul, Jonathan

    2012-08-01

    In this manuscript we analyze the collective behavior of mean-field limits of large-scale, spatially extended stochastic neuronal networks with delays. Rigorously, the asymptotic regime of such systems is characterized by a very intricate stochastic delayed integro-differential McKean-Vlasov equation that remain impenetrable, leaving the stochastic collective dynamics of such networks poorly understood. In order to study these macroscopic dynamics, we analyze networks of firing-rate neurons, i.e. with linear intrinsic dynamics and sigmoidal interactions. In that case, we prove that the solution of the mean-field equation is Gaussian, hence characterized by its two first moments, and that these two quantities satisfy a set of coupled delayed integro-differential equations. These equations are similar to usual neural field equations, and incorporate noise levels as a parameter, allowing analysis of noise-induced transitions. We identify through bifurcation analysis several qualitative transitions due to noise in the mean-field limit. In particular, stabilization of spatially homogeneous solutions, synchronized oscillations, bumps, chaotic dynamics, wave or bump splitting are exhibited and arise from static or dynamic Turing-Hopf bifurcations. These surprising phenomena allow further exploring the role of noise in the nervous system.

  17. Infection Rate and Tissue Localization of Murine IL-12p40-Producing Monocyte-Derived CD103+ Lung Dendritic Cells during Pulmonary Tuberculosis

    PubMed Central

    Leepiyasakulchai, Chaniya; Taher, Chato; Chuquimia, Olga D.; Mazurek, Jolanta; Söderberg-Naucler, Cecilia; Fernández, Carmen; Sköld, Markus

    2013-01-01

    Non-hematopoietic cells, including lung epithelial cells, influence host immune responses. By co-culturing primary alveolar epithelial cells and monocytes from naïve donor mice, we show that alveolar epithelial cells support monocyte survival and differentiation in vitro, suggesting a role for non-hematopoietic cells in monocyte differentiation during the steady state in vivo. CD103+ dendritic cells (αE-DC) are present at mucosal surfaces. Using a murine primary monocyte adoptive transfer model, we demonstrate that αE-DC in the lungs and pulmonary lymph nodes are monocyte-derived during pulmonary tuberculosis. The tissue localization may influence the functional potential of αE-DC that accumulate in Mycobacterium tuberculosis-infected lungs. Here, we confirm the localization of αE-DC in uninfected mice beneath the bronchial epithelial cell layer and near the vascular wall, and show that αE-DC have a similar distribution in the lungs during pulmonary tuberculosis and are detected in the bronchoalveolar lavage fluid from infected mice. Lung DC can be targeted by M. tuberculosis in vivo and play a role in bacterial dissemination to the draining lymph node. In contrast to other DC subsets, only a fraction of lung αE-DC are infected with the bacterium. We also show that virulent M. tuberculosis does not significantly alter cell surface expression levels of MHC class II on infected cells in vivo and that αE-DC contain the highest frequency of IL-12p40+ cells among the myeloid cell subsets in infected lungs. Our results support a model in which inflammatory monocytes are recruited into the M. tuberculosis-infected lung tissue and, depending on which non-hematopoietic cells they interact with, differentiate along different paths to give rise to multiple monocyte-derived cells, including DC with a distinctive αE-DC phenotype. PMID:23861965

  18. Communication: Charge-transfer rate constants in zinc-porphyrin-porphyrin-derived dyads: A Fermi golden rule first-principles-based study

    SciTech Connect

    Manna, Arun K.; Dunietz, Barry D.

    2014-09-28

    We investigate photoinduced charge transfer (CT) processes within dyads consisting of porphyrin derivatives in which one ring ligates a Zn metal center and where the rings vary by their degree of conjugation. Using a first-principles approach, we show that molecular-scale means can tune CT rates through stabilization affected by the polar environment. Such means of CT tuning are important for achieving high efficiency optoelectronic applications using organic semiconducting materials. Our fully quantum mechanical scheme is necessary for reliably modeling the CT process across different regimes, in contrast to the pervading semi-classical Marcus picture that grossly underestimates transfer in the far-inverted regime.

  19. Rate of lateral magma transport in the Earth's crust beneath submarine volcanic arcs derived from earthquake swarm analysis

    NASA Astrophysics Data System (ADS)

    Spicak, A.; Vanek, J.

    2015-12-01

    This contribution deals with subduction-related submarine magmatism. We are offering a tool to contribute to delimitation of domains of current magma unrest at convergent plate margins and to understand better the behavior of magma in the lithospheric wedge above the subducting slab: a detailed analysis of teleseismic earthquake occurrence. A specific seismicity pattern has been observed beneath submarine portions of several volcanic arcs at convergent plate margins (Andaman Sea region, southern Ryukyu area). We have found three arguments that allowed us to interpret such a seismicity pattern as a magma-driven process: (i) clustering of medium-size earthquakes (M~5) in space and time in shallow earthquake swarms; (ii) rapid migration of seismic activity during the swarms (comparison of epicentral maps of individual stages of the swarm development showed consistently that earthquake epicenters migrate laterally at a rate of several hundred meters per hour); (iii) correlation of epicentral zones of the swarms with distinct seamounts and submarine ridges (current seismically active intrusions probably propagate along plumbing systems that served as conduits of magma to the surface in the past). The repeated occurrence of relatively strong, teleseismically recorded earthquake swarms thus probably reflects fluid and/or magma ascent through the plumbing system of the volcanic arc, points to brittle character of the lithospheric wedge at respective depths and favors the studied areas - the Andaman Sea region and the southern Ryukyu area - to be potential sites of submarine volcanic activity.. The study documents high accuracy of hypocenter parameter determinations published by data centers such as ISC and NEIC USGS, and the usefulness of the EHB relocation procedure.

  20. K(+) accumulation in the cytoplasm and nucleus of the salt gland cells of Limonium bicolor accompanies increased rates of salt secretion under NaCl treatment using NanoSIMS.

    PubMed

    Feng, Zhong-Tao; Deng, Yun-Quan; Zhang, Shi-Chao; Liang, Xue; Yuan, Fang; Hao, Jia-Long; Zhang, Jian-Chao; Sun, Shu-Feng; Wang, Bao-Shan

    2015-09-01

    Recretohalophytes with specialized salt-secreting structures (salt glands) can secrete excess salts from plant, while discriminating between Na(+) and K(+). K(+)/Na(+) ratio plays an important role in plant salt tolerance, but the distribution and role of K(+) in the salt gland cells is poorly understood. In this article, the in situ subcellular localization of K and Na in the salt gland of the recretohalophyte Limonium bicolor Kuntze is described. Samples were prepared by high-pressure freezing (HPF), freeze substitution (FS) and analyzed using NanoSIMS. The salt gland of L. bicolor consists of sixteen cells. Higher signal strength of Na(+) was located in the apoplast of salt gland cells. Compared with control, 200 mM NaCl treatment led to higher signal strength of K(+) and Na(+) in both cytoplasm and nucleus of salt gland cells although K(+)/Na(+) ratio in both cytoplasm and nucleus were slightly reduced by NaCl. Moreover, the rate of Na(+) secretion per salt gland of L. bicolor treated with 200 mM NaCl was five times that of controls. These results suggest that K(+) accumulation both in the cytoplasm and nucleus of salt gland cells under salinity may play an important role in salt secretion, although the exact mechanism is unknown.

  1. The effect of heating rate on the structural and electrical properties of sol-gel derived Al-doped ZnO films

    NASA Astrophysics Data System (ADS)

    Gao, Meizhen; Wu, Xiaonan; Liu, Jing; Liu, Wenbao

    2011-05-01

    Al-doped ZnO (AZO) films are prepared by sol-gel method with a proper annealing procedure. For the first time, we find that the heating rate which is normally neglected during the post annealing process plays a significant role in improving AZO properties. The AZO film with nanorod structure is obtained by using a rapid heating rate. The AZO nanorods can provide a faster conduction pathway for charge transport due to the high crystal quality and thus enhance the conductivity of the film significantly. After hydrogen treatment, the AZO nanorod film exhibits a minimum resistivity of 1.4 × 10 -3 Ω cm. This approach to the preparation of AZO nanorods by a simple rapid annealing process may be helpful for the development of sol-gel-derived TCO films.

  2. Mapping the Extent and Rate of Overbank Deposition Using Mine-Derived Sediment Tracers Along the Strickland River, Papua New Guinea

    NASA Astrophysics Data System (ADS)

    Apte, S. C.; Dietrich, W. E.; Day, G. M.; Reibe, C.; Aalto, R.; Sanders, J.; Lauer, W.; Simpson, S. L.; Marshall, A.; Bera, M.

    2003-12-01

    As part of collaborative investigation among an NSF sponsored Margins Source to Sink research group, University of Papua New Guinea (PNG) researchers, Porgera Joint Venture (PNG), Ok Tedi Mining Ltd (PNG) and CSIRO (Australia), a field study is being conducted to map the extent and rate of overbank deposition along the Strickland River, which is the dominant drainage to the Fly River delta, Papua New Guinea. A key question is what proportion of the river's sediment load is deposited on the lowland floodplain before the river joins the Fly. Previous studies on the Fly River above the junction with the Strickland concluded that about 40% of the lowland sediment load is lost to the floodplain. It was hypothesized that the historically much higher load on the Strickland had lead to more rapid infilling of accommodation space and consequently current rates of deposition would be proportionately smaller than on the Fly. The Strickland River receives inputs of mine tailings and waste rock from the Porgera gold mine, which is situated at an elevation of 2,500 meters in the upper catchment of the river system. While the contribution to the overall sediment load of the river is small, the mine-derived sediments contain elevated concentrations of several metals and there is both a need to understand their transport, fate and the potential release of dissolved metals as they are transported through the system and an opportunity to use these metals as tracers of sediment transport and deposition. This paper summarizes studies undertaken to examine the extent of overbank deposition of mine-derived sediments in the lower reaches of the Strickland River. Sediment cores were collected along transects at six key points on the lower Strickland River floodplain during 1997 and 2003 and analyzed for a suite of trace elements. In 2003, additional cores were collected at 5 other transects and duplicate cores were collected at all transects for analysis of 210Pb. Particulate silver and

  3. Derivation of a true (t → 0+) quantum transition-state theory. II. Recovery of the exact quantum rate in the absence of recrossing.

    PubMed

    Althorpe, Stuart C; Hele, Timothy J H

    2013-08-28

    In Paper I [T. J. H. Hele and S. C. Althorpe, J. Chem. Phys. 138, 084108 (2013)] we derived a quantum transition-state theory (TST) by taking the t → 0+ limit of a new form of quantum flux-side time-correlation function containing a ring-polymer dividing surface. This t → 0+ limit appears to be unique in giving positive-definite Boltzmann statistics, and is identical to ring-polymer molecular dynamics (RPMD) TST. Here, we show that quantum TST (i.e., RPMD-TST) is exact if there is no recrossing (by the real-time quantum dynamics) of the ring-polymer dividing surface, nor of any surface orthogonal to it in the space describing fluctuations in the polymer-bead positions along the reaction coordinate. In practice, this means that RPMD-TST gives a good approximation to the exact quantum rate for direct reactions, provided the temperature is not too far below the cross-over to deep tunnelling. We derive these results by comparing the t → ∞ limit of the ring-polymer flux-side time-correlation function with that of a hybrid flux-side time-correlation function (containing a ring-polymer flux operator and a Miller-Schwarz-Tromp side function), and by representing the resulting ring-polymer momentum integrals as hypercubes. Together with Paper I, the results of this article validate a large number of RPMD calculations of reaction rates.

  4. Organic-Carbon Sequestration in Soil/Sediment of the Mississippi River Deltaic Plain - Data; Landscape Distribution, Storage, and Inventory; Accumulation Rates; and Recent Loss, Including a Post-Katrina Preliminary Analysis (Chapter B)

    USGS Publications Warehouse

    Markewich, Helaine W.; Buell, Gary R.; Britsch, Louis D.; McGeehin, John P.; Robbins, John A.; Wrenn, John H.; Dillon, Douglas L.; Fries, Terry L.; Morehead, Nancy R.

    2007-01-01

    Soil/sediment of the Mississippi River deltaic plain (MRDP) in southeastern Louisiana is rich in organic carbon (OC). The MRDP contains about 2 percent of all OC in the surface meter of soil/sediment in the Mississippi River Basin (MRB). Environments within the MRDP differ in soil/sediment organic carbon (SOC) accumulation rate, storage, and inventory. The focus of this study was twofold: (1) develop a database for OC and bulk density for MRDP soil/sediment; and (2) estimate SOC storage, inventory, and accumulation rates for the dominant environments (brackish, intermediate, and fresh marsh; natural levee; distributary; backswamp; and swamp) in the MRDP. Comparative studies were conducted to determine which field and laboratory methods result in the most accurate and reproducible bulk-density values for each marsh environment. Sampling methods included push-core, vibracore, peat borer, and Hargis1 sampler. Bulk-density data for cores taken by the 'short push-core method' proved to be more internally consistent than data for samples collected by other methods. Laboratory methods to estimate OC concentration and inorganic-constituent concentration included mass spectrometry, coulometry, and loss-on-ignition. For the sampled MRDP environments, these methods were comparable. SOC storage was calculated for each core with adequate OC and bulk-density data. SOC inventory was calculated using core-specific data from this study and available published and unpublished pedon data linked to SSURGO2 map units. Sample age was estimated using isotopic cesium (137Cs), lead (210Pb), and carbon (14C), elemental Pb, palynomorphs, other stratigraphic markers, and written history. SOC accumulation rates were estimated for each core with adequate age data. Cesium-137 profiles for marsh soil/sediment are the least ambiguous. Levee and distributary 137Cs profiles show the effects of intermittent allochthonous input and/or sediment resuspension. Cesium-137 and 210Pb data gave the most

  5. Quantifying uncertainty in morphologically-derived bedload transport rates for large braided rivers: insights from high-resolution, high-frequency digital elevation model differencing

    NASA Astrophysics Data System (ADS)

    Brasington, J.; Hicks, M.; Wheaton, J. M.; Williams, R. D.; Vericat, D.

    2013-12-01

    Repeat surveys of channel morphology provide a means to quantify fluvial sediment storage and enable inferences about changes in long-term sediment supply, watershed delivery and bed level adjustment; information vital to support effective river and land management. Over shorter time-scales, direct differencing of fluvial terrain models may also offer a route to predict reach-averaged sediment transport rates and quantify the patterns of channel morphodynamics and the processes that force them. Recent and rapid advances in geomatics have facilitated these goals by enabling the acquisition of topographic data at spatial resolutions and precisions suitable for characterising river morphology at the scale of individual grains over multi-kilometre reaches. Despite improvements in topographic surveying, inverting the terms of the sediment budget to derive estimates of sediment transport and link these to morphodynamic processes is, nonetheless, often confounded by limited knowledge of either the sediment supply or efflux across a boundary of the control volume, or unobserved cut-and-fill taking place between surveys. This latter problem is particularly poorly constrained, as field logistics frequently preclude surveys at a temporal frequency sufficient to capture changes in sediment storage associated with each competent event, let alone changes during individual floods. In this paper, we attempt to quantify the principal sources of uncertainty in morphologically-derived bedload transport rates for the large, labile, gravel-bed braided Rees River which drains the Southern Alps of NZ. During the austral summer of 2009-10, a unique timeseries of 10 high quality DEMs was derived for a 3 x 0.7 km reach of the Rees, using a combination of mobile terrestrial laser scanning, aDcp soundings and aerial image analysis. Complementary measurements of the forcing flood discharges and estimates of event-based particle step lengths were also acquired during the field campaign

  6. Magnitude and variability of Holocene sediment accumulation in Santa Monica Bay, California.

    PubMed

    Sommerfield, Christopher K; Lee, Homa J

    2003-01-01

    The spatial variability of Holocene (past 10,000 years) sediment accumulation in Santa Monica Bay (California) was examined to identify controls sediment trapping in a bathymetrically complex coastal embayment and to provide geologic context for the post-industrial sedimentary record and associated pollution gradients. Sediment chronologies based on downcore AMS 14C dates were used to quantify long-term (millennia) accumulation rates in an effort to elucidate particle-transport pathways and sinks. Sediment accumulation rates for the full range of bayfloor environments (50-630 m water depths) range from 22 to 102 mg/cm(2)/year (15-88 mm/100 year), have an overall mean of 51+/-21 mg/cm(2)/year (1 sigma, n=11), and are comparable to rates reported for adjacent borderland basins. Maximal accumulation rates on the Malibu shelf and within a reentrant to Redondo canyon are interpreted to reflect (1) proximity to sediment sources and (2) localized oceanographic and topographic conditions conducive to sediment trapping and deposition. The 14C-derived accumulation rates are 2-10 times lower than rates determined through (210)Pb geochronology for the same sites in a related study, revealing that Holocene sediment accumulation has been non-steady-state. Santa Monica Bay is an important sink for suspended matter; averaged over the past several millennia a mass of sediment equivalent to 10-80% of the modern annual river supply is sequestered yearly. Net influx of suspended matter derived from the adjacent Palos Verdes shelf is evinced by a concentration gradient of p,p'-DDE in bayfloor sediments, whereas the distribution of anthropogenic silver suggests transport from Santa Monica shelf to the southeastern boundary of the bay. The results of this study provide new insight to the long-term fates of particulate matter in Los Angeles coastal waters.

  7. Magnitude and variability of Holocene sediment accumulation in Santa Monica Bay, California

    USGS Publications Warehouse

    Sommerfield, C.K.; Lee, H.J.

    2003-01-01

    The spatial variability of Holocene (past 10,000 years) sediment accumulation in Santa Monica Bay (California) was examined to identify controls sediment trapping in a bathymetrically complex coastal embayment and to provide geologic context for the post-industrial sedimentary record and associated pollution gradients. Sediment chronologies based on downcore AMS 14C dates were used to quantify long-term (millennia) accumulation rates in an effort to elucidate particle-transport pathways and sinks. Sediment accumulation rates for the full range of bayfloor environments (50-630 m water depths) range from 22 to 102 mg/cm2/year (15-88 mm/100 year), have an overall mean of 51??21 mg/cm2/year (1??, n=11), and are comparable to rates reported for adjacent borderland basins. Maximal accumulation rates on the Malibu shelf and within a reentrant to Redondo canyon are interpreted to reflect (1) proximity to sediment sources and (2) localized oceanographic and topographic conditions conducive to sediment trapping and deposition. The 14C-derived accumulation rates are 2-10 times lower than rates determined through 210Pb geochronology for the same sites in a related study, revealing that Holocene sediment accumulation has been non-steady-state. Santa Monica Bay is an important sink for suspended matter; averaged over the past several millennia a mass of sediment equivalent to 10-80% of the modern annual river supply is sequestered yearly. Net influx of suspended matter derived from the adjacent Palos Verdes shelf is evinced by a concentration gradient of p,p???-DDE in bayfloor sediments, whereas the distribution of anthropogenic silver suggests transport from Santa Monica shelf to the southeastern boundary of the bay. The results of this study provide new insight to the long-term fates of particulate matter in Los Angeles coastal waters. ?? 2003 Elsevier Science Ltd. All rights reserved.

  8. The Influence of Tissue Ischemia Time on RNA Integrity and Patient-Derived Xenografts (PDX) Engraftment Rate in a Non-Small Cell Lung Cancer (NSCLC) Biobank

    PubMed Central

    Maletta, Francesca; Gaudiano, Marcello; Ercole, Elisabetta; Annaratone, Laura; Todaro, Maria; Boita, Monica; Filosso, Pier Luigi; Solidoro, Paolo; Delsedime, Luisa; Oliaro, Alberto; Sapino, Anna; Ruffini, Enrico; Inghirami, Giorgio

    2016-01-01

    Introduction Bio-repositories are invaluable resources to implement translational cancer research and clinical programs. They represent one of the most powerful tools for biomolecular studies of clinically annotated cohorts, but high quality samples are required to generate reliable molecular readouts and functional studies. The objective of our study was to define the impact of cancer tissue ischemia time on RNA and DNA quality, and for the generation of Patient-Derived Xenografts (PDXs). Methods One-hundred thirty-five lung cancer specimens were selected among our Institutional BioBank samples. Associations between different warm (surgical) and cold (ex-vivo) ischemia time ranges and RNA quality or PDXs engraftment rates were assessed. RNA quality was determined by RNA integrity number (RINs) values. Fresh viable tissue fragments were implanted subcutaneously in NSG mice and serially transplanted. Results RNAs with a RIN>7 were detected in 51% of the sample (70/135), with values of RIN significantly lower (OR 0.08, P = 0.01) in samples preserved for more than 3 hours before cryopreservation. Higher quality DNA samples had a concomitant high RIN. Sixty-three primary tumors (41 adenocarcinoma) were implanted with an overall engraftment rate of 33%. Both prolonged warm (>2 hours) and ex-vivo ischemia time (>10 hours) were associated to a lower engraftment rate (OR 0.09 P = 0.01 and OR 0.04 P = 0.008, respectively). Conclusion RNA quality and PDXs engraftment rate were adversely affected by prolonged ischemia times. Proper tissue collection and processing reduce failure rate. Overall, NSCLC BioBanking represents an innovative modality, which can be successfully executed in routine clinical settings, when stringent Standard Operating Procedures are adopted. PMID:26731692

  9. Respiratory rate and pulse oximetry derived information as predictors of hospital admission in young children in Bangladesh: a prospective observational study

    PubMed Central

    Garde, Ainara; Zhou, Guohai; Raihana, Shahreen; Dunsmuir, Dustin; Karlen, Walter; Dekhordi, Parastoo; Huda, Tanvir; Arifeen, Shams El; Larson, Charles; Kissoon, Niranjan; Dumont, Guy A; Ansermino, J Mark

    2016-01-01

    Objective Hypoxaemia is a strong predictor of mortality in children. Early detection of deteriorating condition is vital to timely intervention. We hypothesise that measures of pulse oximetry dynamics may identify children requiring hospitalisation. Our aim was to develop a predictive tool using only objective data derived from pulse oximetry and observed respiratory rate to identify children at increased risk of hospital admission. Setting Tertiary-level hospital emergency department in Bangladesh. Participants Children under 5 years (n=3374) presenting at the facility (October 2012–April 2013) without documented chronic diseases were recruited. 1-minute segments of pulse oximetry (photoplethysmogram (PPG), blood oxygen saturation (SpO2) and heart rate (HR)) and respiratory rate were collected with a mobile app. Primary outcome The need for hospitalisation based on expert physician review and follow-up. Methods Pulse rate variability (PRV) using pulse peak intervals of the PPG signal and features extracted from the SpO2 signal, all derived from pulse oximetry recordings, were studied. A univariate age-adjusted logistic regression was applied to evaluate differences between admitted and non-admitted children. A multivariate logistic regression model was developed using a stepwise selection of predictors and was internally validated using bootstrapping. Results Children admitted to hospital showed significantly (p<0.01) decreased PRV and higher SpO2 variability compared to non-admitted children. The strongest predictors of hospitalisation were reduced PRV-power in the low frequency band (OR associated with a 0.01 unit increase, 0.93; 95% CI 0.89 to 0.98), greater time spent below an SpO2 of 98% and 94% (OR associated with 10 s increase, 1.4; 95% CI 1.3 to 1.4 and 1.5; 95% CI 1.4 to 1.6, respectively), high respiratory rate, high HR, low SpO2, young age and male sex. These variables provided a bootstrap-corrected AUC of the receiver operating characteristic

  10. In defence of the general validity of the Cha method of deriving rate equations. The importance of explicit recognition of the thermodynamic box in enzyme kinetics.

    PubMed Central

    Topham, C M; Brocklehurst, K

    1992-01-01

    1. The suggestion by Segel & Martin [(1988) J. Theor. Biol. 135, 445-453] that the well-known schematic method for the derivation of rate equations for combined quasi-equilibrium-steady-state models proposed by Cha [(1968) J. Biol. Chem. 243, 820-825] may not be generally applicable was shown to be incorrect. By contrast, Cha's method was shown (a) to yield correct initial-rate equations that are exact and (b) not to require any constraints on the relative magnitudes of rate constants for slow steps outside the quasi-equilibrium segments of the kinetic model, including those suggested by Segel & Martin. 2. Examination of residual King-Altman patterns for the general modifier model of Botts & Morales [(1953) Trans. Faraday Soc. 49, 696-707] revealed the reasons for the erroneous conclusions reached by Segel & Martin. The errors arise from the failure to take account of fluxes in parallel pathways that connect the two isolated groups of enzyme species existing in quasi-equilibrium with the modifier. 3. A similar failure to take explicit account of parallel pathways in a thermodynamic box that delayed proper appreciation of the form of pH-dependence of kcat/Km is briefly discussed. PMID:1540141

  11. A comparison of methods for deriving solute flux rates using long-term data from streams in the mirror lake watershed

    USGS Publications Warehouse

    Bukaveckas, P.A.; Likens, G.E.; Winter, T.C.; Buso, D.C.

    1998-01-01

    Calculation of chemical flux rates for streams requires integration of continuous measurements of discharge with discrete measurements of solute concentrations. We compared two commonly used methods for interpolating chemistry data (time-averaging and flow-weighting) to determine whether discrepancies between the two methods were large relative to other sources of error in estimating flux rates. Flux rates of dissolved Si and SO42- were calculated from 10 years of data (1981-1990) for the NW inlet and Outlet of Mirror Lake and for a 40-day period (March 22 to April 30, 1993) during which we augmented our routine (weekly) chemical monitoring with collection of daily samples. The time-averaging method yielded higher estimates of solute flux during high-flow periods if no chemistry samples were collected corresponding to peak discharge. Concentration-discharge relationships should be used to interpolate stream chemistry during changing flow conditions if chemical changes are large. Caution should be used in choosing the appropriate time-scale over which data are pooled to derive the concentration-discharge regressions because the model parameters (slope and intercept) were found to be sensitive to seasonal and inter-annual variation. Both methods approximated solute flux to within 2-10% for a range of solutes that were monitored during the intensive sampling period. Our results suggest that errors arising from interpolation of stream chemistry data are small compared with other sources of error in developing watershed mass balances.

  12. In situ study through electrical resistance of growth rate of trifluoroacetate-based solution-derived YBa2Cu3O7 films

    NASA Astrophysics Data System (ADS)

    Sánchez-Valdés, C. F.; Puig, T.; Obradors, X.

    2015-02-01

    In this work, we have studied by means of in situ electrical measurements the nucleation, growth and sintering stages of epitaxial YBa2Cu3O6+δ (YBCO) superconducting thin films prepared using a chemical solution deposition approach based on metal-organic trifluoroacetate-based (TFA) precursors. Single crystal substrates (LaAlO3 and CeO2/YSZ) were used in this study. Analysis of isothermal time dependences, at different temperatures, of in situ electrical resistance of films allowed to evidence that the growth rate G is strongly temperature dependent, i.e. G is enhanced by a factor ˜15 when going from 700 to 810 °C. Additionally, we demonstrate that adding Ag-TFA in the solution may enhance the growth rate by as much as 50%, as compared to pure YBCO, thus confirming previous assessments of the strong influence of Ag doping on YBCO film growth and microstructure. In situ electrical resistance measurements show as well that an incubation time exists and we infer the origin of its temperature dependence. Finally, a thermodynamic analysis allows proposing a single equation for the growth rate of YBCO films integrating all the relevant processing parameters. Our analysis has validated the solid-gas reaction-diffusion model describing the growth of YBCO films from TFA precursors and thus enlarges the knowledge required to enhance the control of the microstructure and superconducting properties of solution-derived YBCO films.

  13. Calculation of rates for enzyme and microbial kinetics via a spline technique

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In biocatalysis research, determination of enzyme kinetics, microbial growth rates, substrate utilization rates, and product accumulation rates sometime require derivatives to be calculated with a method that can be duplicated and yields consistent results. In this paper, several methods that have ...

  14. Allochthonous and autochthonous contributions to carbon accumulation and carbon store in southeastern Australian coastal wetlands

    NASA Astrophysics Data System (ADS)

    Saintilan, N.; Rogers, K.; Mazumder, D.; Woodroffe, C.

    2013-08-01

    Estimates of carbon store and carbon accumulation rate in mangrove and saltmarsh are beset by issues of scale and provenance. Estimates at a site do not allow scaling to regional estimates if the drivers of variability are not known. Also, carbon accumulation within soils provides a net offset only if carbon is derived in-situ, or would not otherwise be sequestered. We use a network of observation sites extending across 2000 km of southeastern Australian coastline to determine the influence of geomorphic setting and coastal wetland vegetation type on rates of carbon accumulation, carbon store and probable sources. Carbon accumulation above feldspar marker horizons over a 10-year period was driven primarily by tidal range and position in the tidal frame, and was higher for mangrove and saltmarsh dominated by Juncus kraussii than for other saltmarsh communities. The rate of carbon loss with depth varied between geomorphic settings and was the primary determinant of carbon store. A down-core enrichment in δ13C was consistent with an increased relative contribution of mangrove root material to soil carbon, as mangrove roots were found to be consistently enriched compared to leaves. We conclude that while surface carbon accumulation is driven primarily by tidal transport of allocthonous sediment, in-situ carbon sequestration is the dominant source of recalcitrant carbon, and that mangrove and saltmarsh carbon accumulation and store is high in temperate settings, particularly in mesotidal and fluvial geomorphic settings.

  15. Chromoplast biogenesis and carotenoid accumulation.

    PubMed

    Li, Li; Yuan, Hui

    2013-11-15

    Chromoplasts are special organelles that possess superior ability to synthesize and store massive amounts of carotenoids. They are responsible for the distinctive colors found in fruits, flowers, and roots. Chromoplasts exhibit various morphologies and are derived from either pre-existing chloroplasts or other non-photosynthetic plastids such as proplastids, leucoplasts or amyloplasts. While little is known about the molecular mechanisms underlying chromoplast biogenesis, research progress along with proteomics study of chromoplast proteomes signifies various processes and factors important for chromoplast differentiation and development. Chromoplasts act as a metabolic sink that enables great biosynthesis and high storage capacity of carotenoids. The formation of chromoplasts enhances carotenoid metabolic sink strength and controls carotenoid accumulation in plants. The objective of this review is to provide an integrated view on our understanding of chromoplast biogenesis and carotenoid accumulation in plants.

  16. High genetic and epigenetic stability in Coffea arabica plants derived from embryogenic suspensions and secondary embryogenesis as revealed by AFLP, MSAP and the phenotypic variation rate.

    PubMed

    Bobadilla Landey, Roberto; Cenci, Alberto; Georget, Frédéric; Bertrand, Benoît; Camayo, Gloria; Dechamp, Eveline; Herrera, Juan Carlos; Santoni, Sylvain; Lashermes, Philippe; Simpson, June; Etienne, Hervé

    2013-01-01

    Embryogenic suspensions that involve extensive cell division are risky in respect to genome and epigenome instability. Elevated frequencies of somaclonal variation in embryogenic suspension-derived plants were reported in many species, including coffee. This problem could be overcome by using culture conditions that allow moderate cell proliferation. In view of true-to-type large-scale propagation of C. arabica hybrids, suspension protocols based on low 2,4-D concentrations and short proliferation periods were developed. As mechanisms leading to somaclonal variation are often complex, the phenotypic, genetic and epigenetic changes were jointly assessed so as to accurately evaluate the conformity of suspension-derived plants. The effects of embryogenic suspensions and secondary embryogenesis, used as proliferation systems, on the genetic conformity of somatic embryogenesis-derived plants (emblings) were assessed in two hybrids. When applied over a 6 month period, both systems ensured very low somaclonal variation rates, as observed through massive phenotypic observations in field plots (0.74% from 200,000 plant). Molecular AFLP and MSAP analyses performed on 145 three year-old emblings showed that polymorphism between mother plants and emblings was extremely low, i.e. ranges of 0-0.003% and 0.07-0.18% respectively, with no significant difference between the proliferation systems for the two hybrids. No embling was found to cumulate more than three methylation polymorphisms. No relation was established between the variant phenotype (27 variants studied) and a particular MSAP pattern. Chromosome counting showed that 7 of the 11 variant emblings analyzed were characterized by the loss of 1-3 chromosomes. This work showed that both embryogenic suspensions and secondary embryogenesis are reliable for true-to-type propagation of elite material. Molecular analyses revealed that genetic and epigenetic alterations are particularly limited during coffee somatic embryogenesis

  17. High Genetic and Epigenetic Stability in Coffea arabica Plants Derived from Embryogenic Suspensions and Secondary Embryogenesis as Revealed by AFLP, MSAP and the Phenotypic Variation Rate

    PubMed Central

    Bobadilla Landey, Roberto; Cenci, Alberto; Georget, Frédéric; Bertrand, Benoît; Camayo, Gloria; Dechamp, Eveline; Herrera, Juan Carlos; Santoni, Sylvain; Lashermes, Philippe; Simpson, June; Etienne, Hervé

    2013-01-01

    Embryogenic suspensions that involve extensive cell division are risky in respect to genome and epigenome instability. Elevated frequencies of somaclonal variation in embryogenic suspension-derived plants were reported in many species, including coffee. This problem could be overcome by using culture conditions that allow moderate cell proliferation. In view of true-to-type large-scale propagation of C. arabica hybrids, suspension protocols based on low 2,4-D concentrations and short proliferation periods were developed. As mechanisms leading to somaclonal variation are often complex, the phenotypic, genetic and epigenetic changes were jointly assessed so as to accurately evaluate the conformity of suspension-derived plants. The effects of embryogenic suspensions and secondary embryogenesis, used as proliferation systems, on the genetic conformity of somatic embryogenesis-derived plants (emblings) were assessed in two hybrids. When applied over a 6 month period, both systems ensured very low somaclonal variation rates, as observed through massive phenotypic observations in field plots (0.74% from 200 000 plant). Molecular AFLP and MSAP analyses performed on 145 three year-old emblings showed that polymorphism between mother plants and emblings was extremely low, i.e. ranges of 0–0.003% and 0.07–0.18% respectively, with no significant difference between the proliferation systems for the two hybrids. No embling was found to cumulate more than three methylation polymorphisms. No relation was established between the variant phenotype (27 variants studied) and a particular MSAP pattern. Chromosome counting showed that 7 of the 11 variant emblings analyzed were characterized by the loss of 1–3 chromosomes. This work showed that both embryogenic suspensions and secondary embryogenesis are reliable for true-to-type propagation of elite material. Molecular analyses revealed that genetic and epigenetic alterations are particularly limited during coffee somatic

  18. An Enhancement of 160um-derived Star-Formation Rates in Active Galaxies to z = 3.2 with the ZFOURGE Survey

    NASA Astrophysics Data System (ADS)

    Cowley, Michael; Spitler, L.

    2016-08-01

    ZFOURGE is a new imaging survey, which employs unique near-infrared medium- band filters designed to probe galaxy properties over the last 12 billion years. By supplementing the high-quality ZFOURGE galaxy catalogues with data in radio, X- ray, and infrared wavebands, we have studied the complex interplay between AGNs and their host galaxies to a time when the Universe was only 10-15% of its present age. I will report on our study to compare the 160-um derived star-formation rates in active and non-active galaxies and show that AGN hosts, over all redshifts, tend to exhibit an elevated average star-formation activity compared to their non-active counterparts.

  19. Novel characterization of monocyte-derived cell populations in the meninges and choroid plexus and their rates of replenishment in bone marrow chimeric mice.

    PubMed

    Chinnery, Holly R; Ruitenberg, Marc J; McMenamin, Paul G

    2010-09-01

    The mouse dura mater, pia mater, and choroid plexus contain resident macrophages and dendritic cells (DCs). These cells participate in immune surveillance, phagocytosis of cellular debris, uptake of antigens from the surrounding cerebrospinal fluid and immune regulation in many pathologic processes. We used Cx3cr1 knock-in, CD11c-eYFP transgenic and bone marrow chimeric mice to characterize the phenotype, density and replenishment rate of monocyte-derived cells in the meninges and choroid plexus and to assess the role of the chemokine receptor CX3CR1 on their number and tissue distribution. Iba-1 major histocompatibility complex (MHC) Class II CD169 CD68 macrophages and CD11c putative DCs were identified in meningeal and choroid plexus whole mounts. Comparison of homozygous and heterozygous Cx3cr1 mice did not reveal CX3CR1-dependancy on density, distribution or phenotype of monocyte-derived cells. In turnover studies, wild type lethally irradiated mice were reconstituted with Cx3cr1/-positive bone marrow and were analyzed at 3 days, 1, 2, 4 and 8 weeks after transplantation. There was a rapid replenishment of CX3CR1-positive cells in the dura mater (at 4 weeks) and the choroid plexus was fully reconstituted by 8 weeks. These data provide the foundation for future studies on the role of resident macrophages and DCs in conditions such as meningitis, autoimmune inflammatory disease and in therapies involving irradiation and hematopoietic or stem cell transplantation.

  20. Which plant trait explains the variations in relative growth rate and its response to elevated carbon dioxide concentration among Arabidopsis thaliana ecotypes derived from a variety of habitats?

    PubMed

    Oguchi, Riichi; Ozaki, Hiroshi; Hanada, Kousuke; Hikosaka, Kouki

    2016-03-01

    Elevated atmospheric carbon dioxide (CO2) concentration ([CO2]) enhances plant growth, but this enhancement varies considerably. It is still uncertain which plant traits are quantitatively related to the variation in plant growth. To identify the traits responsible, we developed a growth analysis model that included primary parameters associated with morphology, nitrogen (N) use, and leaf and root activities. We analysed the vegetative growth of 44 ecotypes of Arabidopsis thaliana L. grown at ambient and elevated [CO2] (800 μmol mol(-1)). The 44 ecotypes were selected such that they were derived from various altitudes and latitudes. Relative growth rate (RGR; growth rate per unit plant mass) and its response to [CO2] varied by 1.5- and 1.7-fold among ecotypes, respectively. The variation in RGR at both [CO2]s was mainly explained by the variation in leaf N productivity (LNP; growth rate per leaf N),which was strongly related to photosynthetic N use efficiency (PNUE). The variation in the response of RGR to [CO2] was also explained by the variation in the response of LNP to [CO2]. Genomic analyses indicated that there was no phylogenetic constraint on inter-ecotype variation in the CO2 response of RGR or LNP. We conclude that the significant variation in plant growth and its response to [CO2] among ecotypes reflects the variation in N use for photosynthesis among ecotypes, and that the response of PNUE to CO2 is an important target for predicting and/or breeding plants that have high growth rates at elevated [CO2].

  1. The Relationship of a Combination of Human Adipose Tissue-Derived Stem Cells and Frozen Fat with the Survival Rate of Transplanted Fat

    PubMed Central

    Ha, Ki-Young; Park, Hojin; Park, Seung-Ha; Lee, Byung-Il; Ji, Yi-Hwa; Kim, Tae-Yeon

    2015-01-01

    Background The survival rate of grafted fat is difficult to predict, and repeated procedures are frequently required. In this study, the effects of the freezing period of harvested adipose tissue and the addition of human adipose tissue-derived stem cells (ASCs) on the process of fat absorption were studied. Methods Adipose tissue was obtained from patients who underwent a lipoaspirated fat graft. The fat tissue was cryopreserved at -20℃ in a domestic refrigerator. A total of 40 nude mice were used. The mice in the experimental group received three different subcutaneous injections in the back: an injection of fresh fat and ASCs, an injection of fat that had been frozen for one month and ASCs, and an injection of fat that had been frozen for two months and ASCs. The control mice received fat grafts without ASCs. The mice were sacrificed at four or eight weeks after the procedure, and the grafted fat tissues were harvested. The extracted fat was evaluated using photographic analysis, volume measurements, and histological examination. Results In the control group, the fat resorption rates four weeks after transplantation in the grafts of fresh fat, fat that had been frozen for one month, and fat that had been frozen for two months were 21.14%, 22.46%, and 42.56%, respectively. In the experimental group, the corresponding resorption rates were 6.68%, 13.0%, and 33.9%, respectively. Conclusions ASCs can increase the fat graft survival rate. The use of ASCs in fat grafting can reduce the need for repeated fat grafts and provide good long term results. PMID:26618113

  2. 19 CFR 10.534 - Accumulation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. United States-Singapore Free Trade Agreement Rules of Origin § 10.534 Accumulation. (a) Originating materials of Singapore or the United States...

  3. 19 CFR 10.534 - Accumulation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. United States-Singapore Free Trade Agreement Rules of Origin § 10.534 Accumulation. (a) Originating materials of Singapore or the United States...

  4. 19 CFR 10.534 - Accumulation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. United States-Singapore Free Trade Agreement Rules of Origin § 10.534 Accumulation. (a) Originating materials of Singapore or the United States...

  5. 19 CFR 10.534 - Accumulation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. United States-Singapore Free Trade Agreement Rules of Origin § 10.534 Accumulation. (a) Originating materials of Singapore or the United States...

  6. 19 CFR 10.534 - Accumulation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. United States-Singapore Free Trade Agreement Rules of Origin § 10.534 Accumulation. (a) Originating materials of Singapore or the United States...

  7. Analysis of migration rate and chemotaxis of human adipose-derived mesenchymal stem cells in response to LPS and LTA in vitro.

    PubMed

    Herzmann, Nicole; Salamon, Achim; Fiedler, Tomas; Peters, Kirsten

    2016-03-15

    Mesenchymal stem cells (MSC) are able to stimulate the regeneration of injured tissue. Since bacterial infections are common complications in wound healing, bacterial pathogens and their components come into direct contact with MSC. The interaction with bacterial structures influences the proliferation, differentiation and migratory activity of the MSC, which might be of relevance during regeneration. Studies on MSC migration in response to bacterial components have shown different results depending on the cell type. Here, we analyzed the migration rate and chemotaxis of human adipose-derived MSC (adMSC) in response to the basic cell-wall components lipopolysaccharide (LPS) of Gram-negative bacteria and lipoteichoic acid (LTA) of Gram-positive bacteria in vitro. To this end, we used transwell and scratch assays, as well as a specific chemotaxis assay combined with live-cell imaging. We found no significant influence of LPS or LTA on the migration rate of adMSC in transwell or scratch assays. Furthermore, in the µ-slide chemotaxis assay, the stimulation with LPS did not exert any chemotactic effect on adMSC.

  8. Ignition delays, heats of combustion, and reaction rates of aluminum alkyl derivatives used as ignition and combustion enhancers for supersonic combustion

    NASA Technical Reports Server (NTRS)

    Ryan, Thomas W., III; Schwab, S. T.; Harlowe, W. W.

    1992-01-01

    The subject of this paper is the design of supersonic combustors which will be required in order to achieve the needed reaction rates in a reasonable sized combustor. A fuel additive approach, which is the focus of this research, is the use of pyrophorics to shorten the ignition delay time and to increase the energy density of the fuel. Pyrophoric organometallic compounds may also provide an ignition source and flame stabilization mechanism within the combustor, thus permitting use of hydrocarbon fuels in supersonic combustion systems. Triethylaluminum (TEA) and trimethylaluminum (TMA) were suggested for this application due to their high energy density and reactivity. The objective here is to provide comparative data for the ignition quality, the energy content, and the reaction rates of several different adducts of both TEA and TMA. The results of the experiments indicate the aluminum alkyls and their more stable derivatives reduce the ignition delay and total reaction time to JP-10 jet fuel. Furthermore, the temperature dependence of ignition delay and total reaction time of the blends of the adducts are significantly lower than in neat JP-10.

  9. The empirical relationship between satellite-derived tropospheric NO2 and fire radiative power and possible implications for fire emission rates of NOx

    NASA Astrophysics Data System (ADS)

    Schreier, S. F.; Richter, A.; Kaiser, J. W.; Burrows, J. P.

    2014-03-01

    Nitrogen oxides (NOx) play key roles in atmospheric chemistry, air pollution, and climate. While the largest fraction of these reactive gases is released by anthropogenic emission sources, a significant amount can be attributed to vegetation fires. In this study, NO2 from GOME-2 on board EUMETSAT's MetOp-A and OMI on board NASA's Aura as well as fire radiative power (FRP) from the measurements of MODIS on board NASA's Terra and Aqua satellites are used to derive fire emission rates (FERs) of NOx for different types of vegetation using a simple statistical approach. Monthly means of tropospheric NO2 vertical columns (TVC NO2) have been analyzed for their temporal correlation with the monthly means of FRP for five consecutive years from 2007 to 2011 on a horizontal 1° × 1° grid. The strongest correlation is found to be largely confined to tropical and subtropical regions, which account for more than 80% of yearly burned area, on average, globally. In these regions, the seasonal variation of fire intensity, expressed by the FRP data, is similar to the pattern of TVC NO2. As chemical models typically require values for the amount of NOx being released as a function of time, we have converted the retrieved TVC NO2 into production rates of NOx from fire (Pf) by assuming a constant lifetime of NOx. The comparison between Pf and NOx emissions from the Global Fire Emissions Database (GFEDv3.1) over 5 characteristic biomass burning regions in the tropics and subtropics shows good agreement. By separating the monthly means of Pf and FRP according to land cover type, FERs of NOx could be derived for different biomes. The estimated FERs for the dominating types of vegetation burned are lowest for open shrublands and savannas (0.28-1.03 g NOx s-1 MW-1) and highest for croplands and woody savannas (0.82-1.56 g NOx s-1 MW-1). This analysis demonstrates that the strong empirical relationship between TVC NO2 and FRP and the following simplified assumptions are a useful tool for

  10. Cryopreservation of Human Wharton’s Jelly-derived Mesenchymal Stem Cells Following Controlled Rate Freezing Protocol Using Different Cryoprotectants; A Comparative Study

    PubMed Central

    Shivakumar, Sharath Belame; Bharti, Dinesh; Jang, Si-Jung; Hwang, Sun-Chul; Park, Ji-Kwon; Shin, Jeong-Kyu; Byun, June-Ho; Park, Bong-Wook; Rho, Gyu-Jin

    2015-01-01

    Objectives To compare the effect of three different cryoprotectants on basic stem cell characteristics for the possibility of using well defined, dimethyl sulfoxide (DMSO) and serum free freezing solutions to cryopreserve human Wharton’s jelly-derived mesenchymal stem cells (WJMSCs) following controlled rate freezing protocol. Methods The mesenchymal stem cells isolated from human Wharton’s jelly were cryopreserved using 10% DMSO, 10% polyvinylpyrrolidone (PVP) and a cocktail solution comprising of 0.05 M glucose, 0.05 M sucrose and 1.5 M ethylene glycol following controlled rate freezing protocol. We investigated the post-thaw cell viability, morphology, proliferation capacity, basic stem cell characteristics, in vitro differentiation potential and apoptosis-related gene expression profile before and after cryopreservation. Results The cryoprotectant 10% DMSO has shown higher post-thaw cell viability of 81.2±0.58% whereas 10% PVP and cocktail solution have shown 62.87±0.35% and 72.2±0.23%, respectively at 0 h immediately thawing. The cell viability was further reduced in all the cryopreserved groups at 24 h later post-thaw culture. Further, the complete elimination of FBS in cryoprotectants has resulted in drastic reduction in cell viability. Cryopreservation did not alter the basic stem cell characteristics, plasticity and multipotency except proliferation rate. The expression of pro-apoptotic BAX and p53 genes were higher whilst p21 was lower in all the cryopreserved groups when compare to the control group of WJMSCs. Conclusion Although 10% DMSO has shown higher post-thaw cell viability compare to 10% PVP and cocktail solution, the present study indicates the feasibility of developing a well-defined DMSO free cryosolution which can improve storage and future broad range applications of WJMSCs in regenerative medicine without losing their basic stem cell characteristics. PMID:26634064

  11. Experimental constraints on the serpentinization rate of fore-arc peridotites: Implications for the upwelling condition of the slab-derived fluid

    NASA Astrophysics Data System (ADS)

    Nakatani, T.; Nakamura, M.

    2016-08-01

    To constrain the water circulation in subduction zones, the hydration rates of peridotites were investigated experimentally in fore-arc mantle conditions. Experiments were conducted at 400-580°C and 1.3 and 1.8 GPa, where antigorite is expected to form as a stable serpentine phase. Crushed powders of olivine ± orthopyroxene and orthopyroxene + clinopyroxene were reacted with 15 wt % distilled water for 4-19 days. The synthesized serpentine varieties were lizardite and aluminous lizardite (Al-lizardite) in all experimental conditions except those of 1.8 GPa and 580°C in the olivine + orthopyroxene system, in which antigorite was formed. In the olivine + orthopyroxene system, the reactions were interface-controlled except for the reaction at 400°C, which was transport-controlled. The corresponding reaction rates were 7.0 × 10-12 to 1.5 × 10-11 m s-1 at 500-580°C and 7.5 × 10-16 m2 s-1 at 400°C for the interface and transport-controlled reactions, respectively. Based on a simple reaction-transport model including these hydration rates, we infer that penetration of the slab-derived fluid all the way through a water-unsaturated fore-arc mantle is allowed only when focused flow occurs with a spacing larger than 77-229 km in hot subduction zones such as Nankai and Cascadia. However, the necessary spacing is only 2.3-4.6 m in intermediate-temperature subduction zones such as Kyushu and Costa Rica. These calculations imply that fluid leakage in hot subduction zones may occur after the fore-arc mantle is totally hydrated, whereas in intermediate-temperature subduction zones, leakage through a water-unsaturated fore-arc mantle may be facilitated.

  12. Fire emission rates of NOx based on the empirical relationship between satellite-derived tropospheric NO2 and fire radiative power

    NASA Astrophysics Data System (ADS)

    Schreier, S. F.; Richter, A.; Kaiser, J. W.; Burrows, J. P.

    2013-11-01

    Nitrogen oxides (NOx) play key roles in atmospheric chemistry, air pollution, and climate. While the largest fraction of these reactive gases is released by anthropogenic emission sources, a significant amount can be attributed to vegetation fires. In this study, NO2 from GOME-2 on board EUMETSAT's MetOp-A and OMI on board NASA's Aura as well as fire radiative power (FRP) from the measurements of MODIS on board NASA's Terra and Aqua are used to derive fire emission rates (FERs) of NOx for different types of vegetation using a simple statistical approach. Monthly means of tropospheric NO2 vertical columns (TVC NO2) have been analyzed for their temporal correlation with the monthly means of FRP for five consecutive years from 2007 to 2011 on a horizontal 1° × 1° grid. The strongest correlation is found to be largely confined to tropical and subtropical regions, which account for more than 80% of yearly burned area on average globally. In these regions, the seasonal variation of fire intensity, expressed by the FRP data, is similar to the pattern of TVC NO2. As chemical models typically require values for the amount of NOx being released as a function of time, we have converted the retrieved TVC NO2 into production rates of NOx from fire (Pf) by assuming a constant lifetime of NOx. The comparison between Pf and NOx emissions from GFEDv3.1 over 5 characteristic biomass burning regions in the tropics and subtropics indicated good agreement. By separating the monthly means of Pf and FRP according to land cover type, FERs of NOx could be derived for different biomes. The estimated FERs for the dominating types of vegetation burned are lowest for open shrublands and savannas (0.28-1.03 g NOx s-1 MW-1) and highest for croplands and woody savannas (0.82-1.56 g NOx s-1 MW-1). This analysis demonstrates clearly that there are biome-specific, diurnal, and regional differences in FERs for the dominating types of vegetation burned in the tropics and subtropics. Possible

  13. Potential of On-Line Visible and Near Infrared Spectroscopy for Measurement of pH for Deriving Variable Rate Lime Recommendations

    PubMed Central

    Tekin, Yücel; Kuang, Boyan; Mouazen, Abdul M.

    2013-01-01

    This paper aims at exploring the potential of visible and near infrared (vis-NIR) spectroscopy for on-line measurement of soil pH, with the intention to produce variable rate lime recommendation maps. An on-line vis-NIR soil sensor set up to a frame was used in this study. Lime application maps, based on pH predicted by vis-NIR techniques, were compared with maps based on traditional lab-measured pH. The validation of the calibration model using off-line spectra provided excellent prediction accuracy of pH (R2 = 0.85, RMSEP = 0.18 and RPD = 2.52), as compared to very good accuracy obtained with the on-line measured spectra (R2 = 0.81, RMSEP = 0.20 and RPD = 2.14). On-line predicted pH of all points (e.g., 2,160) resulted in the largest overall field virtual lime requirement (1.404 t), as compared to those obtained with 16 validation points off-line prediction (0.28 t), on-line prediction (0.14 t) and laboratory reference measurement (0.48 t). The conclusion is that the vis-NIR spectroscopy can be successfully used for the prediction of soil pH and for deriving lime recommendations. The advantage of the on-line sensor over sampling with limited number of samples is that more detailed information about pH can be obtained, which is the reason for a higher but precise calculated lime recommendation rate. PMID:23966186

  14. A trial of production of the plant-derived high-value protein in a plant factory: photosynthetic photon fluxes affect the accumulation of recombinant miraculin in transgenic tomato fruits.

    PubMed

    Kato, Kazuhisa; Maruyama, Shinichiro; Hirai, Tadayoshi; Hiwasa-Tanase, Kyoko; Mizoguchi, Tsuyoshi; Goto, Eiji; Ezura, Hiroshi

    2011-08-01

    One of the ultimate goals of plant science is to test a hypothesis obtained by basic science and to apply it to agriculture and industry. A plant factory is one of the ideal systems for this trial. Environmental factors affect both plant yield and the accumulation of recombinant proteins for industrial applications within transgenic plants. However, there have been few reports studying plant productivity for recombinant protein in closed cultivation systems called plant factories. To investigate the effects of photosynthetic photon flux (PPF) on tomato fruit yield and the accumulation of recombinant miraculin, a taste-modifying glycoprotein, in transgenic tomato fruits, plants were cultivated at various PPFs from 100 to 400 (µmol m(-2) s(-)1) in a plant factory. Miraculin production per unit of energy used was highest at PPF100, although miraculin production per unit area was highest at PPF300. The commercial productivity of recombinant miraculin in transgenic tomato fruits largely depended on light conditions in the plant factory. Our trial will be useful to consider the trade-offs between the profits from production of high-value materials in plants and the costs of electricity. PMID:21791976

  15. A trial of production of the plant-derived high-value protein in a plant factory: photosynthetic photon fluxes affect the accumulation of recombinant miraculin in transgenic tomato fruits.

    PubMed

    Kato, Kazuhisa; Maruyama, Shinichiro; Hirai, Tadayoshi; Hiwasa-Tanase, Kyoko; Mizoguchi, Tsuyoshi; Goto, Eiji; Ezura, Hiroshi

    2011-08-01

    One of the ultimate goals of plant science is to test a hypothesis obtained by basic science and to apply it to agriculture and industry. A plant factory is one of the ideal systems for this trial. Environmental factors affect both plant yield and the accumulation of recombinant proteins for industrial applications within transgenic plants. However, there have been few reports studying plant productivity for recombinant protein in closed cultivation systems called plant factories. To investigate the effects of photosynthetic photon flux (PPF) on tomato fruit yield and the accumulation of recombinant miraculin, a taste-modifying glycoprotein, in transgenic tomato fruits, plants were cultivated at various PPFs from 100 to 400 (µmol m(-2) s(-)1) in a plant factory. Miraculin production per unit of energy used was highest at PPF100, although miraculin production per unit area was highest at PPF300. The commercial productivity of recombinant miraculin in transgenic tomato fruits largely depended on light conditions in the plant factory. Our trial will be useful to consider the trade-offs between the profits from production of high-value materials in plants and the costs of electricity.

  16. The Influence of Fuel Moisture, Charge Size, Burning Rate and Air Ventilation Conditions on Emissions of PM, OC, EC, Parent PAHs, and Their Derivatives from Residential Wood Combustion

    PubMed Central

    Shen, Guofeng; Xue, Miao; Wei, Siye; Chen, Yuanchen; Wang, Bin; Wang, Rong; Lv, Yan; Shen, Huizhong; Li, Wei; Zhang, Yanyan; Huang, Ye; Chen, Han; Wei, Wen; Zhao, Qiuyue; Li, Bin; Wu, Haisuo; TAO, Shu

    2014-01-01

    Controlled combustion experiments were conducted to investigate the influence of fuel charge size, moisture, air ventilation and burning rate on the emission factors (EFs) of carbonaceous particulate matter, parent polycyclic aromatic hydrocarbons (pPAHs) and their derivatives from residential wood combustion in a typical brick cooking stove. Measured EFs were found to be independent of fuel charge size, but increased with increasing fuel moisture. Pollution emissions from a normal burning under an adequate air supply condition were the lowest for most pollutants, while more pollutants were emitted when the oxygen deficient atmosphere was formed in stove chamber during fast burning. The impact of these 4 factors on particulate matter size distribution was also studied. Modified combustion efficiency and the four investigated factors explained 68, 72, and 64% of total variations in EFs of PM, organic carbon, and oxygenated PAHs, respectively, but only 36, 38 and 42% of the total variations in EFs of elemental carbon, pPAHs and nitro-PAHs, respectively. PMID:24520723

  17. Is stress accumulating on the creeping section of the San Andreas fault?

    NASA Astrophysics Data System (ADS)

    Johnson, K. M.

    2013-12-01

    The creeping section of the San Andreas fault (CSAF) in central California is a proposed barrier to propagation of large earthquakes. Yet, recent studies show that the creeping section is not entirely uncoupled but is accumulating slip deficit at a rate equivalent to a Mw=7.2-7.4 earthquake every 150years. A critical piece to understanding earthquake potential on the CSAF is determining whether slip deficit is occurring with stress accumulation on stick‒slip regions or without stress accumulation on stable‒sliding regions shadowed by surrounding locked areas. We use a physical model to estimate the spatial distribution of locked, stress‒accumulating areas of the fault constrained by surface creep rate measurements and GPS‒derived velocities. We find that the area of the fault accumulating stress, if ruptured every 150years, would release slip equivalent to at most a Mw=6.75 earthquake, significantly less than the Mw=7.2-7.4, 150year equivalent total slip deficit rate.

  18. Gene limiting cadmium accumulation in rice.

    PubMed

    Ueno, Daisei; Yamaji, Naoki; Kono, Izumi; Huang, Chao Feng; Ando, Tsuyu; Yano, Masahiro; Ma, Jian Feng

    2010-09-21

    Intake of toxic cadmium (Cd) from rice caused Itai-itai disease in the past and it is still a threat for human health. Therefore, control of the accumulation of Cd from soil is an important food-safety issue, but the molecular mechanism for the control is unknown. Herein, we report a gene (OsHMA3) responsible for low Cd accumulation in rice that was isolated from a mapping population derived from a cross between a high and low Cd-accumulating cultivar. The gene encodes a transporter belonging to the P(1B)-type ATPase family, but shares low similarity with other members. Heterologous expression in yeast showed that the transporter from the low-Cd cultivar is functional, but the transporter from the high-Cd cultivar had lost its function, probably because of the single amino acid mutation. The transporter is mainly expressed in the tonoplast of root cells at a similar level in both the low and high Cd-accumulating cultivars. Overexpression of the functional gene from the low Cd-accumulating cultivar selectively decreased accumulation of Cd, but not other micronutrients in the grain. Our results indicated that OsHMA3 from the low Cd-accumulating cultivar limits translocation of Cd from the roots to the above-ground tissues by selectively sequestrating Cd into the root vacuoles.

  19. Loess is the accumulation of dust, not evidence for aridity

    NASA Astrophysics Data System (ADS)

    Zech, Roland

    2013-04-01

    Loess-paleosol sequences (LPS) are valuable terrestrial archives for Quaternary climate and environmental changes. The famous sections on the Chinese Loess Plateau, for example, document the alternation of warm and humid interglacials (paleosols) and cold and more arid glacials (loess). This, at least partly, reflects the weakening of the monsoonal circulation during glacials and has led to the notion that loess in general documents more arid conditions. Paleosols, on the other hand, are often interpreted to document more humid conditions. We studied the LPS Crvenka in the Carpathian Basin, southeast Europe, which spans the full last glacial cycle, and obtained results that do not fit the above concept: (i) The analysis of plant-derived long-chain n-alkanes indicates the presence of deciduous trees and shrubs during glacials, i.e. sufficient precipitation for tree growth, whereas tree-less grass steppes seem to have prevailed during the Eemian, the last interglacial. (ii) Compound-specific deuterium analyses on the alkanes show only little changes on glacial-interglacial timescale. When compared with the isotopic enrichment of the Mediterranean Sea during the last glacial, this likely documents a combination of increased rainfall, reduced evapo-transpiration and reduced temperatures. (iii) Novel lipid biomarkers derived from soil bacteria (GDGTs, glycerol dialkyl glycerol tetraethers) also indicate humid glacials (BIT index close to 1) and more arid interglacials (BIT<0.8). Our results are in good agreement with modelling studies suggesting a southward shift of the westerlies during glacials, and aridization in the Mediterranean area in response to man-made global warming. More importantly, they remind us of an important fact: Loess is the accumulation of dust, but not (necessarily) evidence for aridity. Pedogenesis may simply not have been able to keep pace with high glacial dust accumulation rates related to intense glacial, periglacial and fluvial activity

  20. The empirical relationship between satellite-derived tropospheric NO2 and fire radiative power and possible implications for fire emission rates of NOx

    NASA Astrophysics Data System (ADS)

    Schreier, Stefan F.; Richter, Andreas; Kaiser, Johannes W.; Schepaschenko, Dmitry; Shvidenko, Anatoly; Hilboll, Andreas; Burrows, John P.

    2014-05-01

    Vegetation fires across the globe have various impacts on Earth systems such as the atmosphere and biosphere. Every year, large quantities of biomass in different ecosystems are burned, either started by lightning strikes or caused by humans. Consequently, a considerable amount of trace gases (e.g. NOx) and aerosols is released into the atmosphere. As nitrogen oxides (NOx) affect atmospheric chemistry, air quality, and climate, a quantification of the total emissions is needed. Although several approaches have been developed for the estimation of NOx emissions from fires, they still suffer from large uncertainties. We present a simple statistical approach to estimate fire emission rates (FERs) of NOx based on the linear relationship between satellite-observed tropospheric NO2 vertical columns (TVC NO2) and fire radiative power (FRP). While the great advantage of the method is the spatial coverage of FERs and the application to various biomes and regions, the uncertainties in the two retrieved parameters can lead to uncertainties in the FERs. In general, the approach performs well for the tropical and subtropical regions where both the number and the spatial extent of vegetation fires are rather large throughout the fire season. However, due to the smaller number of fires and the patchy spatial occurrence, the estimation of FERs is more complicated in the boreal regions. Nevertheless, it is possible to derive FERs for some characteristic regions in the North American and Eurasian part of the boreal forest biome. The estimated FERs of NOx for the dominating types of vegetation burned are lowest for open shrublands, savannas, and boreal forest (0.28-1.03 g NOx s-1 MW-1) and highest for croplands and woody savannas (0.82-1.56 g NOx s-1 MW-1). Interestingly, there are large regional discrepancies of up to 40 % observed for evergreen broadleaf forest and boreal forest. Possible explanations for these regional discrepancies are discussed.

  1. Neurodegeneration with Brain Iron Accumulation

    MedlinePlus

    ... Diversity Find People About NINDS NINDS Neurodegeneration with Brain Iron Accumulation Information Page Synonym(s): Hallervorden-Spatz Disease, ... done? Clinical Trials Organizations What is Neurodegeneration with Brain Iron Accumulation? Neurodegeneration with brain iron accumulation (NBIA) ...

  2. Plastids and Carotenoid Accumulation.

    PubMed

    Li, Li; Yuan, Hui; Zeng, Yunliu; Xu, Qiang

    2016-01-01

    Plastids are ubiquitously present in plants and are the organelles for carotenoid biosynthesis and storage. Based on their morphology and function, plastids are classified into various types, i.e. proplastids, etioplasts, chloroplasts, amyloplasts, and chromoplasts. All plastids, except proplastids, can synthesize carotenoids. However, plastid types have a profound effect on carotenoid accumulation and stability. In this chapter, we discuss carotenoid biosynthesis and regulation in various plastids with a focus on carotenoids in chromoplasts. Plastid transition related to carotenoid biosynthesis and the different capacity of various plastids to sequester carotenoids and the associated effect on carotenoid stability are described in light of carotenoid accumulation in plants. PMID:27485226

  3. Plastids and Carotenoid Accumulation.

    PubMed

    Li, Li; Yuan, Hui; Zeng, Yunliu; Xu, Qiang

    2016-01-01

    Plastids are ubiquitously present in plants and are the organelles for carotenoid biosynthesis and storage. Based on their morphology and function, plastids are classified into various types, i.e. proplastids, etioplasts, chloroplasts, amyloplasts, and chromoplasts. All plastids, except proplastids, can synthesize carotenoids. However, plastid types have a profound effect on carotenoid accumulation and stability. In this chapter, we discuss carotenoid biosynthesis and regulation in various plastids with a focus on carotenoids in chromoplasts. Plastid transition related to carotenoid biosynthesis and the different capacity of various plastids to sequester carotenoids and the associated effect on carotenoid stability are described in light of carotenoid accumulation in plants.

  4. Accumulation and turnover of carbon in organic and mineral soils of the BOREAS northern study area

    USGS Publications Warehouse

    Trumbore, S.E.; Harden, J.W.

    1997-01-01

    Rates of input, accumulation, and turnover of C differ markedly within soil profiles and in soils with different drainage in the BOREAS northern study area. Soil C storage increases from ???3 kg C m-2 in well-drained, sandy soils to greater than 100 kg C m-2 in wetlands. Two modes of C accumulation were observed in upland soil profiles. Large annual C inputs (0.06-0.1 kg C m-2 yr-1) and slow decomposition (turnover times of 6-250 years) lead to rapid C accumulation in regrowing surface moss and detrital layers following fire. Deep organic layers that have accumulated over the millennia since the initiation of soil development, and are located below the most recent charred horizon, show slower rates of input (0.015-0.03 kg C m-2 yr-1) and turnover (100-1600 years) and accumulate C about 10 times slower than surface detrital layers. Rates of C input to soils derived from C and 14C data were in accord with net primary production estimates, with highest rates of input (0.14-0.6 kg C m-2 yr-1) in wetlands. Turnover times for C in surface detrital layers were 6-15 years for well-drained sand soils that showed highest soil temperatures in summer, 30-40 years for wetlands, and 36-250 years for uplands with thick moss cover and black spruce trees. Long (>100 years) turnover times in upland black spruce/clay soils most likely reflect the influence of woody debris incorporated into detrital layers. Turnover times for deep organic and mineral layer C were controlled by drainage, with fastest turnover (80-130 years) in well-drained sand soils and slowest turnover (>3000 years) in wetlands. Total C accumulation rates, which account for C losses from both deep organic and surface detrital layers, are close to zero for sand/jack pine soils, 0.003-0.01 kg C m-2 yr-1 for moderately to poorly drained sites in mature forest stands, and 0.03 kg C m-2 yr-1 for a productive fen. Decomposition of organic matter more than several decades old accounts for 9-22% of total heterotrophic

  5. Strain patterns and strain accumulation along plate margins

    NASA Technical Reports Server (NTRS)

    Savage, J. C.

    1978-01-01

    Observations of strain accumulation along plate margins in Japan, New Zealand, and the United States indicate that: (1) a typical maximum rate of secular strain accumulation is on the order of 0.3 ppm/a, (2) a substantial part of the strain accumulation process can be attributed to slip at depth on the major plate boundary faults, and (3) some plastic deformation in a zone 100 km or more in width is apparently involved in the strain accumulation process.

  6. Chimpanzee accumulative stone throwing.

    PubMed

    Kühl, Hjalmar S; Kalan, Ammie K; Arandjelovic, Mimi; Aubert, Floris; D'Auvergne, Lucy; Goedmakers, Annemarie; Jones, Sorrel; Kehoe, Laura; Regnaut, Sebastien; Tickle, Alexander; Ton, Els; van Schijndel, Joost; Abwe, Ekwoge E; Angedakin, Samuel; Agbor, Anthony; Ayimisin, Emmanuel Ayuk; Bailey, Emma; Bessone, Mattia; Bonnet, Matthieu; Brazolla, Gregory; Buh, Valentine Ebua; Chancellor, Rebecca; Cipoletta, Chloe; Cohen, Heather; Corogenes, Katherine; Coupland, Charlotte; Curran, Bryan; Deschner, Tobias; Dierks, Karsten; Dieguez, Paula; Dilambaka, Emmanuel; Diotoh, Orume; Dowd, Dervla; Dunn, Andrew; Eshuis, Henk; Fernandez, Rumen; Ginath, Yisa; Hart, John; Hedwig, Daniela; Ter Heegde, Martijn; Hicks, Thurston Cleveland; Imong, Inaoyom; Jeffery, Kathryn J; Junker, Jessica; Kadam, Parag; Kambi, Mohamed; Kienast, Ivonne; Kujirakwinja, Deo; Langergraber, Kevin; Lapeyre, Vincent; Lapuente, Juan; Lee, Kevin; Leinert, Vera; Meier, Amelia; Maretti, Giovanna; Marrocoli, Sergio; Mbi, Tanyi Julius; Mihindou, Vianet; Moebius, Yasmin; Morgan, David; Morgan, Bethan; Mulindahabi, Felix; Murai, Mizuki; Niyigabae, Protais; Normand, Emma; Ntare, Nicolas; Ormsby, Lucy Jayne; Piel, Alex; Pruetz, Jill; Rundus, Aaron; Sanz, Crickette; Sommer, Volker; Stewart, Fiona; Tagg, Nikki; Vanleeuwe, Hilde; Vergnes, Virginie; Willie, Jacob; Wittig, Roman M; Zuberbuehler, Klaus; Boesch, Christophe

    2016-01-01

    The study of the archaeological remains of fossil hominins must rely on reconstructions to elucidate the behaviour that may have resulted in particular stone tools and their accumulation. Comparatively, stone tool use among living primates has illuminated behaviours that are also amenable to archaeological examination, permitting direct observations of the behaviour leading to artefacts and their assemblages to be incorporated. Here, we describe newly discovered stone tool-use behaviour and stone accumulation sites in wild chimpanzees reminiscent of human cairns. In addition to data from 17 mid- to long-term chimpanzee research sites, we sampled a further 34 Pan troglodytes communities. We found four populations in West Africa where chimpanzees habitually bang and throw rocks against trees, or toss them into tree cavities, resulting in conspicuous stone accumulations at these sites. This represents the first record of repeated observations of individual chimpanzees exhibiting stone tool use for a purpose other than extractive foraging at what appear to be targeted trees. The ritualized behavioural display and collection of artefacts at particular locations observed in chimpanzee accumulative stone throwing may have implications for the inferences that can be drawn from archaeological stone assemblages and the origins of ritual sites.

  7. Chimpanzee accumulative stone throwing