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Sample records for accumulation rates derived

  1. Ice shelf snow accumulation rates from the Amundsen-Bellingshausen Sea sector of West Antarctica derived from airborne radar

    NASA Astrophysics Data System (ADS)

    Medley, B.; Kurtz, N. T.; Brunt, K. M.

    2015-12-01

    The large ice shelves surrounding the Antarctic continent buttress inland ice, limiting the grounded ice-sheet flow. Many, but not all, of the thick ice shelves located along the Amundsen-Bellingshausen Seas are experiencing rapid thinning due to enhanced basal melting driven by the intrusion of warm circumpolar deep water. Determination of their mass balance provides an indicator as to the future of the shelves buttressing capability; however, measurements of surface accumulation are few, limiting the precision of the mass balance estimates. Here, we present new radar-derived measurements of snow accumulation primarily over the Getz and Abbott Ice Shelves, as well as the Dotson and Crosson, which have been the focus of several of NASA's Operation IceBridge airborne surveys between 2009 and 2014. Specifically, we use the Center for Remote Sensing of Ice Sheets (CReSIS) snow radar to map the near-surface (< 30 m) internal stratigraphy to measure snow accumulation. Due to the complexities of the local topography (e.g., ice rises and rumples) and their relative proximity to the ocean, the spatial pattern of accumulation can be equally varied. Therefore, atmospheric models might not be able to reproduce these small-scale features because of their limited spatial resolution. To evaluate whether this is the case over these narrow shelves, we will compare the radar-derived accumulation rates with those from atmospheric models.

  2. Net accumulation rates derived from ice core stable isotope records of Pío XI glacier, Southern Patagonia Icefield

    NASA Astrophysics Data System (ADS)

    Schwikowski, M.; Schläppi, M.; Santibañez, P.; Rivera, A.; Casassa, G.

    2012-12-01

    Pío XI, the largest glacier of the Southern Patagonia Icefield, reached its neoglacial maximum extent in 1994 and is one of the few glaciers in that area which is not retreating. In view of the recent warming it is important to understand glacier responses to climate changes. Due to its remoteness and the harsh conditions in Patagonia, no systematic mass balance studies have been performed. In this study we derived net accumulation rates for the period 2000 to 2006 from a 50 m (33.2 4 m weq) ice core collected in the accumulation area of Pío XI (2600 m a.s.l., 49°16´40´´ S, 73°21´14´´ W). Borehole temperatures indicate near temperate ice, but the average melt percent is only 16% ± 14%. Records of stable isotopes are well preserved and were used for identification of annual layers. Net accumulation rates range from 3.4 to 7.1 water equivalent (m weq) with an average of 5.8 m weq, comparable to precipitation amounts at the Chilean coast, but not as high as expected for the Icefield. Ice core stable isotope data correlate well with upper air temperatures and may be used as temperature proxy.

  3. Accumulation rates during 1311-2011 CE in North Central Greenland derived from air-borne radar data

    NASA Astrophysics Data System (ADS)

    Karlsson, Nanna; Eisen, Olaf; Dahl-Jensen, Dorthe; Freitag, Johannes; Kipfstuhl, Sepp; Lewis, Cameron; Nielsen, Lisbeth; Paden, John; Winter, Anna; Wilhelms, Frank

    2016-11-01

    Radar-detected internal layering contains information on past accumulation rates and patterns. In this study, we assume that the radar layers are isochrones, and use the layer stratigraphy in combination with ice-core measurements and numerical methods to retrieve accumulation information for the northern part of central Greenland. Measurements of the dielectric properties of an ice core from the NEEM (North Greenland Eemian Ice Drilling) site, allow for correlation of the radar layers with volcanic horizons to obtain an accurate age of the layers. We obtain accumulation patterns averaged over 100 a for the period 1311-2011. Our results show a clear trend of high accumulation rates west of the ice divide and low accumulation rates east of the ice divide. At the NEEM site the accumulation pattern is persistent during our study period and only small temporal variations occur in the accumulation rate. However, from approximately 200 km south of the NEEM drill site, the accumulation rate shows temporal variations based on our centennial averages. We attribute this variation to shifts in the location of the high-low accumulation boundary that usually is aligned with the ice divide, but appears to have moved across the divide in the past.

  4. Comparison of annual accumulation rates derived from in situ and ground penetrating radar methods across Alaskan glaciers

    NASA Astrophysics Data System (ADS)

    McGrath, D.; Gusmeroli, A.; Oneel, S.; Sass, L. C.; Arendt, A. A.; Wolken, G. J.; Kienholz, C.; McNeil, C.

    2013-12-01

    Constraining annual snowfall accumulation in mountain glacier environments is essential for determining the annual mass balance of individual glaciers and predicting seasonal meltwater runoff to river and marine ecosystems. However, large spatial and elevation gradients, coupled with sparse point measurements preclude accurate quantification of this variable using traditional methods. Here, we report on an extensive field campaign conducted in March-May 2013 on key benchmark glaciers in Alaska, including Taku Glacier near Juneau, Scott Glacier near Cordova, both Eklutna and Wolverine Glacier near Anchorage and Gulkana Glacier in the interior Alaska Range. Over 50 km of 500 MHz common-offset ground penetrating radar (GPR) surveys were collected on each glacier, with an emphasis on capturing spatial variability in the accumulation zone. Frequent in situ observations were collected for comparison with the GPR, including probe depths, snow pits and shallow firn cores (~8 m). We report on spatial and elevation gradients across this suite of glaciers and across numerous climatic zones and discuss differences between GPR and in situ derived annual accumulation estimates. This comparison is an essential first step in order to effectively evaluate regional atmospheric re-analysis products.

  5. Soil weathering and accumulation rates of oxalate-extractable phases derived from alpine chronosequences of up to 1 Ma in age

    NASA Astrophysics Data System (ADS)

    Dahms, Dennis; Favilli, Filippo; Krebs, Rolf; Egli, Markus

    2012-05-01

    In this study we compare newly-developed chemical weathering data with previously published data from soils developed along two chronosequences of glacial deposits in the European Alps and the Rocky Mountains (Wind River Range, USA). By combining these chronosequences, we are able to present a comprehensive dataset that represents a time period of > 1 Ma. We describe weathering trends of important elements using a number of weathering indices (e.g., K + Ca/Ti ratio, the weathering 'index B' of Kronberg and Nesbitt (1981) and the open mass transport function). Further, we describe the accumulation of Al, Fe, Si and Mn oxyhydroxides (including partially organic phases) as a function of time, and derive the corresponding accumulation rates. We calculated pedogenetically formed oxyhydroxides using an approach based on immobile elements. Our study represents one of only a few studies that describe rates of soil chemical weathering over a period as long as ~ 1 Ma. Results show that rates of chemical weathering clearly decrease along the chronosequences with increasing age of the soils. We find weathering rates are nearly four orders of magnitude lower in the 1 Ma-old soils than in the young soils. Our results suggest that the older soils may be reaching a steady state for these chemical properties in their present environments. A power function best explains the measured time-trends of the 'index B' and (K + Ca)/Ti) ratios in the soils. The best time-trend model for pedogenic weakly- to poorly crystalline phases and the relative losses/gains (based on the open-system mass transport function) were obtained with an exponential decay model function. In terms of the soil system, the decreases in the accumulation rate of the oxyhydroxides appears to be influenced not only by the factor of time but by climate as well (increased precipitation at higher altitudes slows the decrease in weathering rate over time). Thus, our ~ 1 Ma chronosequences also become pedogenic gradients

  6. Biogeochemical cycling in an organic-rich coastal marine basin: 9. Sources and accumulation rates of vascular plant-derived organic material

    NASA Astrophysics Data System (ADS)

    Haddad, Robert I.; Martens, Christopher S.

    1987-11-01

    The sources, degradation and burial of vascular plant debris deposited over the past several decades in the lagoonal sediments of Cape Lookout Bight, North Carolina, are quantified using alkaline cupric oxide lignin oxidation product (LOP) analysis. Non-woody angiosperms, accounting for 92 ± 32% of the recognizable sedimentary vascular plant debris, are calculated to contribute 23 ± 17% of the total organic carbon buried over the past decade (upper meter of sediment column). When combined with a previously established sedimentary organic carbon budget for this site (Martens and Klump, 1984; Martens et al., 1987, in preparation) a vascular plant derived carbon burial rate of 26 ± 20 mole C m -2 yr -1 is calculated for this same time interval. The refractory nature and invariant depth distributions of the lignin oxidation products (LOP), when coupled with evidence for constant degradation rates of metabolizable materials, indicate that sediment accumulation at this site has been a steady state process with respect to source and burial of organic carbon since its conversion from an inner-continental shelf to a lagoonal environment during the late 1960's. Thus systematic down-core decreases in labile organic matter result from early diagenetic processes rather than input rate variations.

  7. Variation of Accumulation Rates Over the Last Eight Centuries on the East Antarctic Plateau Derived from Volcanic Signals in Ice Cores

    NASA Technical Reports Server (NTRS)

    Anschuetz, H.; Sinisalo, A.; Isaksson, E.; McConnell, J. R.; Hamran, S.-E.; Bisiaux, M. M.; Pasteris, D.; Neumann, T. A.; Winther, J.-G.

    2011-01-01

    Volcanic signatures in ice-core records provide an excellent means to date the cores and obtain information about accumulation rates. From several ice cores it is thus possible to extract a spatio-temporal accumulation pattern. We show records of electrical conductivity and sulfur from firn cores from the Norwegian-USA scientific traverse during the International Polar Year 2007-2009 (IPY) through East Antarctica. Major volcanic eruptions are identified and used to assess century-scale accumulation changes. The largest changes seem to occur in the most recent decades with accumulation over the period 1963- 2007/08 being up to 25 % different from the long-term record. There is no clear overall trend, some sites show an increase in accumulation over the period 1963 to present while others show a decrease. Almost all of the sites above 3200 m above sea level (asl) suggest a decrease. These sites also show a significantly lower accumulation value than large-scale assessments both for the period 1963 to present and for the long-term mean at the respective drill sites. The spatial accumulation distribution is influenced mainly by elevation and distance to the ocean (continentality), as expected. Ground-penetrating radar data around the drill sites show a spatial variability within 10-20 % over several tens of kilometers, indicating that our drill sites are well representative for the area around them. Our results are important for large-scale assessments of Antarctic mass balance and model validation.

  8. Determining Greenland Ice Sheet Accumulation Rates from Radar Remote Sensing

    NASA Technical Reports Server (NTRS)

    Jezek, Kenneth C.

    2002-01-01

    An important component of NASA's Program for Arctic Regional Climate Assessment (PARCA) is a mass balance investigation of the Greenland Ice Sheet. The mass balance is calculated by taking the difference between the areally Integrated snow accumulation and the net ice discharge of the ice sheet. Uncertainties in this calculation Include the snow accumulation rate, which has traditionally been determined by interpolating data from ice core samples taken from isolated spots across the ice sheet. The sparse data associated with ice cores juxtaposed against the high spatial and temporal resolution provided by remote sensing , has motivated scientists to investigate relationships between accumulation rate and microwave observations as an option for obtaining spatially contiguous estimates. The objective of this PARCA continuation proposal was to complete an estimate of surface accumulation rate on the Greenland Ice Sheet derived from C-band radar backscatter data compiled in the ERS-1 SAR mosaic of data acquired during, September-November, 1992. An empirical equation, based on elevation and latitude, is used to determine the mean annual temperature. We examine the influence of accumulation rate, and mean annual temperature on C-band radar backscatter using a forward model, which incorporates snow metamorphosis and radar backscatter components. Our model is run over a range of accumulation and temperature conditions. Based on the model results, we generate a look-up table, which uniquely maps the measured radar backscatter, and mean annual temperature to accumulation rate. Our results compare favorably with in situ accumulation rate measurements falling within our study area.

  9. Temperature-dependence of biomass accumulation rates during secondary succession.

    PubMed

    Anderson, Kristina J; Allen, Andrew P; Gillooly, James F; Brown, James H

    2006-06-01

    Rates of ecosystem recovery following disturbance affect many ecological processes, including carbon cycling in the biosphere. Here, we present a model that predicts the temperature dependence of the biomass accumulation rate following disturbances in forests. Model predictions are derived based on allometric and biochemical principles that govern plant energetics and are tested using a global database of 91 studies of secondary succession compiled from the literature. The rate of biomass accumulation during secondary succession increases with average growing season temperature as predicted based on the biochemical kinetics of photosynthesis in chloroplasts. In addition, the rate of biomass accumulation is greater in angiosperm-dominated communities than in gymnosperm-dominated ones and greater in plantations than in naturally regenerating stands. By linking the temperature-dependence of photosynthesis to the rate of whole-ecosystem biomass accumulation during secondary succession, our model and results provide one example of how emergent, ecosystem-level rate processes can be predicted based on the kinetics of individual metabolic rate.

  10. Deriving in vivo biotransformation rate constants and metabolite parent concentration factor/stable metabolite factor from bioaccumulation and bioconcentration experiments: An illustration with worm accumulation data.

    PubMed

    Kuo, Dave Ta Fu; Chen, Ciara Chun

    2016-12-01

    Growing concern for the biological fate of organic contaminants and their metabolites and the urge to connect in vitro and in vivo toxicokinetics have prompted researchers to characterize the biotransformation behavior of organic contaminants in biota. The whole body biotransformation rate constant (kM ) is currently determined by the difference approach, which has significant methodological limitations. A new approach for determining kM from the kinetic observations of the parent contaminant and its intermediate metabolites is proposed. In this method, kM can be determined by fitting kinetic data of the parent contaminant and the metabolites to analytical equations that depict the bioaccumulation kinetics. The application of the proposed method is illustrated using worm bioaccumulation-biotransformation data collected from the literature. Furthermore, a metabolite parent concentration factor (MPCF) is also proposed to characterize the persistence of the metabolite in biota. Because both the proposed kM method and MPCF build on the existing theoretical framework for bioaccumulation, they can be readily incorporated into standard experimental bioaccumulation protocols or risk assessment procedures or frameworks. Possible limitations, implications, and future directions are elaborated. Environ Toxicol Chem 2016;35:2903-2909. © 2016 SETAC.

  11. Annual Greenland accumulation rates (2009-2012) from airborne snow radar

    NASA Astrophysics Data System (ADS)

    Koenig, Lora S.; Ivanoff, Alvaro; Alexander, Patrick M.; MacGregor, Joseph A.; Fettweis, Xavier; Panzer, Ben; Paden, John D.; Forster, Richard R.; Das, Indrani; McConnell, Joesph R.; Tedesco, Marco; Leuschen, Carl; Gogineni, Prasad

    2016-08-01

    Contemporary climate warming over the Arctic is accelerating mass loss from the Greenland Ice Sheet through increasing surface melt, emphasizing the need to closely monitor its surface mass balance in order to improve sea-level rise predictions. Snow accumulation is the largest component of the ice sheet's surface mass balance, but in situ observations thereof are inherently sparse and models are difficult to evaluate at large scales. Here, we quantify recent Greenland accumulation rates using ultra-wideband (2-6.5 GHz) airborne snow radar data collected as part of NASA's Operation IceBridge between 2009 and 2012. We use a semiautomated method to trace the observed radiostratigraphy and then derive annual net accumulation rates for 2009-2012. The uncertainty in these radar-derived accumulation rates is on average 14 %. A comparison of the radar-derived accumulation rates and contemporaneous ice cores shows that snow radar captures both the annual and long-term mean accumulation rate accurately. A comparison with outputs from a regional climate model (MAR) shows that this model matches radar-derived accumulation rates in the ice sheet interior but produces higher values over southeastern Greenland. Our results demonstrate that snow radar can efficiently and accurately map patterns of snow accumulation across an ice sheet and that it is valuable for evaluating the accuracy of surface mass balance models.

  12. Annual Greenland Accumulation Rates (2009-2012) from Airborne Snow Radar

    NASA Technical Reports Server (NTRS)

    Koenig, Lora S.; Ivanoff, Alvaro; Alexander, Patrick M.; MacGregor, Joseph A.; Fettweis, Xavier; Panzer, Ben; Paden, John D.; Forster, Richard R.; Das, Indrani; McConnell, Joseph R.; Tedesco, Marco; Leuschen, Carl; Gogineni, Prasad

    2016-01-01

    Contemporary climate warming over the Arctic is accelerating mass loss from the Greenland Ice Sheet through increasing surface melt, emphasizing the need to closely monitor its surface mass balance in order to improve sea-level rise predictions. Snow accumulation is the largest component of the ice sheet's surface mass balance, but in situ observations thereof are inherently sparse and models are difficult to evaluate at large scales. Here, we quantify recent Greenland accumulation rates using ultra-wideband (2-6.5 gigahertz) airborne snow radar data collected as part of NASA's Operation IceBridge between 2009 and 2012. We use a semi-automated method to trace the observed radiostratigraphy and then derive annual net accumulation rates for 2009-2012. The uncertainty in these radar-derived accumulation rates is on average 14 percent. A comparison of the radarderived accumulation rates and contemporaneous ice cores shows that snow radar captures both the annual and longterm mean accumulation rate accurately. A comparison with outputs from a regional climate model (MAR - Modele Atmospherique Regional for Greenland and vicinity) shows that this model matches radar-derived accumulation rates in the ice sheet interior but produces higher values over southeastern Greenland. Our results demonstrate that snow radar can efficiently and accurately map patterns of snow accumulation across an ice sheet and that it is valuable for evaluating the accuracy of surface mass balance models.

  13. Determining Greenland Ice Sheet Accumulation Rates from Radar Remote Sensing

    NASA Technical Reports Server (NTRS)

    Jezek, Kenneth C.

    2001-01-01

    An important component of NASA's Program for Arctic Regional Climate Assessment (PARCA) is a mass balance investigation of the Greenland Ice Sheet. The mass balance is calculated by taking the difference between the snow accumulation and the ice discharge of the ice sheet. Uncertainties in this calculation include the snow accumulation rate, which has traditionally been determined by interpolating data from ice core samples taken throughout the ice sheet. The sparse data associated with ice cores, coupled with the high spatial and temporal resolution provided by remote sensing, have motivated scientists to investigate relationships between accumulation rate and microwave observations.

  14. Holocene and recent sediment accumulation rates in southern Lake Michigan

    USGS Publications Warehouse

    Colman, Steven M.; King, J.W.; Jones, Glenn A.; Reynolds, R.L.; Bothner, Michael H.

    2000-01-01

    Rates of sediment accumulation in Lake Michigan are a key component of its geologic history and provide important data related to societal concerns such as shoreline erosion and the fate of anthropogenic pollutants. Previous attempts to reconstruct Holocene rates of sediment accumulation in Lake Michigan, as well as in the other Laurentian Great Lakes, have been bedeviled by the effect of refractory terrestrial material on radiocarbon ages from total organic carbon samples of lake sediments. AMS radiocarbon ages on small samples of biogenic carbonate (ostracodes and mollusks) in Lake Michigan provide accurate Holocene ages. The present bicarbonate reservoir effect is estimated from shells of mollusks collected live before atmospheric nuclear testing to be 250 yr. From paired samples of biogenic carbonate and terrestrial macrofossils, the past reservoir effect is thought to be less than 500 yr. The radiocarbon ages indicate a distinct decrease in sediment accumulation rates throughout the southern basin of Lake Michigan at about 5 ka, about the time when lake level stabilized at the Nipissing level after rising rapidly for several thousand years. Average rates of sediment accumulation for the historic period (the last 150 yr) can be estimated from radioisotopes (210Pb and 137Cs), pollen stratigraphy, and changes in sediment properties associated with human activity. Multiple methods are necessary because at any given site, problems arise in the assumptions or applicability of one or more methods. In general, the mass accumulation calculations suggest that sediments were deposited 4 to 11 times faster in the historic period than before human settlement. The character of the sediment did not change in a dramatic way, but sediment magnetic properties suggest shifts in the sources of sediment. The data suggest that some of the changes in sources and (or) character of the sediment occurred just before human settlement and were probably related to climatic changes

  15. Radon exhalation rates from building materials using electret ion chamber radon monitors in accumulators.

    PubMed

    Kotrappa, Payasada; Stieff, Frederick

    2009-08-01

    An electret ion chamber (EIC) radon monitor in a sealed accumulator measures the integrated average radon concentration at the end of the accumulation duration. Theoretical equations have been derived to relate such radon concentrations (Bq m(-3) ) to the radon emanation rate (Bq d(-1)) from building materials enclosed in the accumulator. As an illustration, a 4-L sealable glass jar has been used as an accumulator to calculate the radon emanation rate from different granite samples. The radon emanation rate was converted into radon flux (Bq mm(-2) d(-1)) by dividing the emanation rate by surface area of the sample. Fluxes measured on typical, commercially available granites ranged from 20-30 Bq m(-2) d(-1). These results are similar to the results reported in the literature. The lower limit of detection for a 2-d measurement works out to be 7 Bq m(-2) d(-1). Equations derived can also be used for other sealable accumulators and other integrating detectors, such as alpha track detectors.

  16. A flight experiment to determine GPS photochemical contamination accumulation rates

    NASA Technical Reports Server (NTRS)

    Tribble, A. C.; Haffner, J. W.

    1990-01-01

    It was recently suggested that photochemically deposited contamination, originating from volatiles outgassed by a spacecraft, may be responsible for the anomalous degradation in power seen on the GPS Block 1 vehicles. In an attempt to confirm, or deny, the photochemical deposition rates predicted, a study was undertaken to design a flight experiment to be incorporated on the GPS vehicles currently in production. The objective was to develop an inexpensive, light weight instrument package that would give information on the contamination levels within a few months of launch. Three types of apparatus were studied, Quartz Crystal Microbalances, (QCM's), modified solar cells, and calorimeters. A calorimeter was selected due primarily to its impact on the production schedule of the GPS vehicles. An analysis of the sensitivity of the final design is compared to the predicted contamination accumulation rates in order to determine how long after launch it will take the experiment to show the effects of photochemical contamination.

  17. An integral method to estimate the moment accumulation rate on the Creeping Section of the San Andreas Fault

    NASA Astrophysics Data System (ADS)

    Tong, Xiaopeng; Sandwell, David T.; Smith-Konter, Bridget

    2015-10-01

    Moment accumulation rate (also referred to as moment deficit rate) is a fundamental quantity for evaluating seismic hazard. The conventional approach for evaluating moment accumulation rate of creeping faults is to invert for the slip distribution from geodetic measurements, although even with perfect data these slip-rate inversions are non-unique. In this study, we show that the slip-rate versus depth inversion is not needed because moment accumulation rate can be estimated directly from surface geodetic data. We propose an integral approach that uses dense geodetic observations from Interferometric Synthetic Aperture Radar (InSAR) and the Global Positioning System (GPS) to constrain the moment accumulation rate. The moment accumulation rate is related to the integral of the product of the along-strike velocity and the distance from the fault. We demonstrate our methods by studying the Creeping Section of the San Andreas fault observed by GPS and radar interferometry onboard the ERS and ALOS satellites. Along-strike variation of the moment accumulation rate is derived in order to investigate the degree of partial locking of the Creeping Section. The central Creeping Segment has a moment accumulation rate of 0.25-3.1 × 1015 Nm yr-1 km-1. The upper and lower bounds of the moment accumulation rates are derived based on the statistics of the noise. Our best-fitting model indicates that the central portion of the Creeping Section is accumulating seismic moment at rates that are about 5 per cent to 23 per cent of the fully locked Carrizo segment that will eventually be released seismically. A cumulative moment budget calculation with the historical earthquake catalogue (M > 5.5) since 1857 shows that the net moment deficit at present is equivalent to a Mw 6.3 earthquake.

  18. Greenland snow accumulation rates estimated by the retracking of percolation facies from airborne radar

    NASA Astrophysics Data System (ADS)

    de la Pena, S.; Howat, I. M.

    2012-12-01

    The margins of the Greenland Ice Sheet are experiencing substantial thinning due to warming in the arctic regions, and there is a growing concern about the effects that mass imbalance of the ice sheet could have on climate and sea level rise. Although volume changes of the ice sheet may be inferred by remote sensing methods, mass gain and accumulation fluctuations are not easily distinguished and are poorly resolved. Recent advances in airborne radar techniques have resulted in systems capable of resolving snow accumulation by retracking internal layers formed by refreezing of surface meltwater that percolates through the snowpack, a phenomenon increasingly common in Greenland. We present accumulation rates for the catchment areas of the Jakobshavn, Helheim, and Rusell glaciers derived from snow depth resolved by snow and Ku-band airborne radar, flown as part of NASA's Operation IceBridge.

  19. Accumulation Rates of Trace Elements in the Cariaco Basin-A 20-kyr History of Seawater Chemistry and Global Climate

    NASA Astrophysics Data System (ADS)

    Piper, D. Z.; Dean, W. E.

    2002-12-01

    A sediment core from the Cariaco Basin on the Venezuelan continental shelf, which collected sediment as old as 20 kyr, was analyzed for its major-element-oxide and trace-element concen-trations. The elements can be partitioned between a siliciclastic, terrigenous-derived fraction and two seawater-derived fractions. The marine fractions are (1) a biogenic fraction represented by nutrient trace elements taken up mostly by phytoplankton in the photic zone, and (2) a hydroge-nous fraction derived from bottom water via adsorption and precipitation reactions. The present-day export of organic matter from the photic zone, redox conditions and advection of bottom water, and the flux of terrigenous debris into the basin are used to calculate current trace-element accu-mulation rates. The sums of calculated accumulation rates of Cd, Cu, Mo, Ni, V, and Zn show excellent agreement with their measured bulk rates of accumulation in the uppermost surface sediment. This agreement between current measured and calculated accumulation rates of trace elements supports a model of trace-element accumulation rates in the subsurface sediment that gives a 20-kyr history of upwelling into the photic zone, bottom-water advection, and sediment provenance. Extrema in the trace-element accumulation rates and interpreted hydrographic properties of the water column correspond to changes in eustatic sea level and global climate.

  20. Sedimentology of Southwestern Roads region, U.S. Virgin Islands: origin and rate of sediment accumulation

    USGS Publications Warehouse

    Kindinger, Jack G.; Miller, Ronald J.; Holmes, Charles W.

    1983-01-01

    Sand deposits on southern insular shelf of St. Thomas, U.S. Virgin Islands, were investigated to determine their origin, environmental processes and accumulation rates. Sea-floor samples show that the sand has been derived (in situ) mainly from calcareous algae and molluscs. Zonation of the dominant sand producers is related to the present environmental setting; water depth has the greatest influence. Carbon-14 data (bulk sample) of cores indicate accumulation rates of slightly less than 1 mm/year for the last 5,000 years. Faunal studies show that the climate has remained constant over the past 5,000 years. The only changes in environmental conditions appear to have been an increase in water depth, changes in the patterns of water movement, and an increase in water temperature.

  1. Synthesis of passive microwave and radar altimeter data for estimating accumulation rates of polar snow

    NASA Technical Reports Server (NTRS)

    Davis, Curt H.

    1993-01-01

    In this paper, we compare dry-snow extinction coefficients derived from radar altimeter data with brightness temperature data from passive microwave measurements over a portion of the East Antarctic plateau. The comparison between the extinction coefficients and the brightness temperatures shows a strong negative correlation, where the correlation coefficients ranged from -0.87 to -0.95. The extinction coefficient of the dry polar snow decreases with increasing surface elevation, while the average brightness temperature increases with surface elevation. Our analysis shows that the observed trends are related to geographic variations in scattering coefficient of snow, which in turn are controlled by variations in surface temperature and snow accumulation rate. By combining information present in the extinction coefficient and brightness temperature data sets, we develop a model that can be used to obtain quantitative estimates of the accumulation rate of dry polar snow.

  2. Rate of tree carbon accumulation increases continuously with tree size

    USGS Publications Warehouse

    Stephenson, N.L.; Das, A.J.; Condit, R.; Russo, S.E.; Baker, P.J.; Beckman, N.G.; Coomes, D.A.; Lines, E.R.; Morris, W.K.; Rüger, N.; Álvarez, E.; Blundo, C.; Bunyavejchewin, S.; Chuyong, G.; Davies, S.J.; Duque, Á.; Ewango, C.N.; Flores, O.; Franklin, J.F.; Grau, H.R.; Hao, Z.; Harmon, M.E.; Hubbell, S.P.; Kenfack, D.; Lin, Y.; Makana, J.-R.; Malizia, A.; Malizia, L.R.; Pabst, R.J.; Pongpattananurak, N.; Su, S.-H.; Sun, I-F.; Tan, S.; Thomas, D.; van Mantgem, P.J.; Wang, X.; Wiser, S.K.; Zavala, M.A.

    2014-01-01

    Forests are major components of the global carbon cycle, providing substantial feedback to atmospheric greenhouse gas concentrations. Our ability to understand and predict changes in the forest carbon cycle—particularly net primary productivity and carbon storage - increasingly relies on models that represent biological processes across several scales of biological organization, from tree leaves to forest stands. Yet, despite advances in our understanding of productivity at the scales of leaves and stands, no consensus exists about the nature of productivity at the scale of the individual tree, in part because we lack a broad empirical assessment of whether rates of absolute tree mass growth (and thus carbon accumulation) decrease, remain constant, or increase as trees increase in size and age. Here we present a global analysis of 403 tropical and temperate tree species, showing that for most species mass growth rate increases continuously with tree size. Thus, large, old trees do not act simply as senescent carbon reservoirs but actively fix large amounts of carbon compared to smaller trees; at the extreme, a single big tree can add the same amount of carbon to the forest within a year as is contained in an entire mid-sized tree. The apparent paradoxes of individual tree growth increasing with tree size despite declining leaf-level and stand-level productivity can be explained, respectively, by increases in a tree’s total leaf area that outpace declines in productivity per unit of leaf area and, among other factors, age-related reductions in population density. Our results resolve conflicting assumptions about the nature of tree growth, inform efforts to understand and model forest carbon dynamics, and have additional implications for theories of resource allocation and plant senescence.

  3. Rate of tree carbon accumulation increases continuously with tree size.

    PubMed

    Stephenson, N L; Das, A J; Condit, R; Russo, S E; Baker, P J; Beckman, N G; Coomes, D A; Lines, E R; Morris, W K; Rüger, N; Alvarez, E; Blundo, C; Bunyavejchewin, S; Chuyong, G; Davies, S J; Duque, A; Ewango, C N; Flores, O; Franklin, J F; Grau, H R; Hao, Z; Harmon, M E; Hubbell, S P; Kenfack, D; Lin, Y; Makana, J-R; Malizia, A; Malizia, L R; Pabst, R J; Pongpattananurak, N; Su, S-H; Sun, I-F; Tan, S; Thomas, D; van Mantgem, P J; Wang, X; Wiser, S K; Zavala, M A

    2014-03-06

    Forests are major components of the global carbon cycle, providing substantial feedback to atmospheric greenhouse gas concentrations. Our ability to understand and predict changes in the forest carbon cycle--particularly net primary productivity and carbon storage--increasingly relies on models that represent biological processes across several scales of biological organization, from tree leaves to forest stands. Yet, despite advances in our understanding of productivity at the scales of leaves and stands, no consensus exists about the nature of productivity at the scale of the individual tree, in part because we lack a broad empirical assessment of whether rates of absolute tree mass growth (and thus carbon accumulation) decrease, remain constant, or increase as trees increase in size and age. Here we present a global analysis of 403 tropical and temperate tree species, showing that for most species mass growth rate increases continuously with tree size. Thus, large, old trees do not act simply as senescent carbon reservoirs but actively fix large amounts of carbon compared to smaller trees; at the extreme, a single big tree can add the same amount of carbon to the forest within a year as is contained in an entire mid-sized tree. The apparent paradoxes of individual tree growth increasing with tree size despite declining leaf-level and stand-level productivity can be explained, respectively, by increases in a tree's total leaf area that outpace declines in productivity per unit of leaf area and, among other factors, age-related reductions in population density. Our results resolve conflicting assumptions about the nature of tree growth, inform efforts to undertand and model forest carbon dynamics, and have additional implications for theories of resource allocation and plant senescence.

  4. Rate of production, dissolution and accumulation of biogenic solids in the ocean

    NASA Technical Reports Server (NTRS)

    Arrhenius, G.

    1988-01-01

    The equatorial current system, by its response to global circulation changes, provides a unique recording mechanism for long range climatic oscillations. A permanent record of the changes in rate of upwelling and organic production is generated in the equatorial deep sea sediments, particularly by such biogenic components which are unaffected by secondary dissolution. In order to determine the rates of accumulation of various sedimentary components, a reliable differential measurement of age of the strata must be obtained. Various approaches to this problem are reviewed, and sources of error discussed. Secondary dissolution of calcium carbonate introduces a substantial and variable difference between the dissolution-modified, and hence a priori unknown, rate of deposition on one hand and the rate of accumulation, derivable from the observed concentration, on the other. The cause and magnitude of these variations are of importance, particularly since some current dating schemes are based on assumed constancy in the rate of accumulation of this and, in some cases, also all other sedimentary components. The concepts used in rate evaluation are discussed with emphasis on the difference between the state of dissolution, an observable property of the sediment, and the rate of dissolution, a parameter that requires deduction of the carbonate fraction dissolved, and of the time differential. As a most likely cause of the enhanced state of dissolution of the interglacial carbonate sediments is proposed the lowered rates of biogenic production and deposition, which cause longer exposure of the carbonate microfossils to corrosion in the bioturbated surface layer of the sediment. Historical perspective is included in the discussion in view of the dedication of the Symposium to Hans Pettersson, the leader of the Swedish Deep Sea Expedition 1947-1948, an undertaking that opened a new era in deep sea research and planetary dynamics.

  5. Rate of production, dissolution and accumulation of biogenic solids in the ocean.

    PubMed

    Arrhenius, G

    1988-01-01

    The equatorial current system, by its response to global circulation changes, provides a unique recording mechanism for long range climatic oscillations. A permanent record of the changes in rate of upwelling and organic production is generated in the equatorial deep sea sediments, particularly by such biogenic components which are unaffected by secondary dissolution. In order to determine the rates of accumulation of various sedimentary components, a reliable differential measurement of age of the strata must be obtained. Various approaches to this problem are reviewed, and sources of error discussed. Secondary dissolution of calcium carbonate introduces a substantial and variable difference between the dissolution-modified, and hence a priori unknown, rate of deposition on one hand and the rate of accumulation, derivable from the observed concentration, on the other. The cause and magnitude of these variations are of importance, particularly since some current dating schemes are based on assumed constancy in the rate of accumulation of this and, in some cases, also all other sedimentary components. The concepts used in rate evaluation are discussed with emphasis on the difference between the state of dissolution, an observable property of the sediment, and the rate of dissolution, a parameter that requires deduction of the carbonate fraction dissolved, and of the time differential. As a most likely cause of the enhanced state of dissolution of the interglacial carbonate sediments is proposed the lowered rates of biogenic production and deposition, which cause longer exposure of the carbonate microfossils to corrosion in the bioturbated surface layer of the sediment. Historical perspective is included in the discussion in view of the dedication of the Symposium to Hans Pettersson, the leader of the Swedish Deep Sea Expedition 1947-1948, an undertaking that opened a new era in deep sea research and planetary dynamics.

  6. Regulation of Activation-associated MicroRNA Accumulation Rates during Monocyte-to-macrophage Differentiation*

    PubMed Central

    Eigsti, Renee L.; Sudan, Bayan; Wilson, Mary E.; Graff, Joel W.

    2014-01-01

    Circulating monocytes recruited to tissues can differentiate into macrophages and adopt unique gene expression programs in response to environmental cues. We recently described the regulated expression of several microRNAs (miRNAs) in polarized human monocyte-derived macrophages (MDMs). Basal expression of these activation-associated miRNAs was low in monocytes relative to MDMs. As development occurs in the context of specific cellular environments, we hypothesized that the rate of miRNA accumulation would be modified in the presence of microbial or cellular products during monocyte-to-macrophage differentiation. Indeed, LPS treatment augmented the accumulation of miR-146a and miR-155, whereas IL-4 treatment augmented the accumulation of miR-193b and miR-222 during development. In contrast, some stimuli repressed accumulation of specific miRNAs including interferons (IFNs) (miR-27a, miR-125a-5p, and miR-222), IL-4 (miR-125a-5p), and LPS (miR-27a). RT-PCR-based expression profiling of monocytes differentiated with distinct methods showed that activation-associated miRNAs and markers of macrophage polarization were substantially altered in MDMs differentiated in the presence of non-monocytic peripheral blood mononuclear cells due in part to NF-κB and STAT1 pathway activation. Expression of several of these miRNAs was regulated at a preprocessing step because the expression of the primary miRNAs, but not Dicer, correlated with mature miRNA expression. We conclude that a set of miRNAs is regulated during MDM differentiation, and the rate is uniquely modified for each miRNA by environmental factors. The low basal expression of activation-associated miRNAs in monocytes and their dynamic rates of accumulation during MDM differentiation permit monocytes to tailor miRNA profiles in peripheral tissues during differentiation to macrophages. PMID:25148686

  7. Accumulation rates from central North Greenland during the past 700 year

    NASA Astrophysics Data System (ADS)

    Karlsson, Nanna B.; Eisen, Olaf; Nielsen, Lisbeth T.; Kipfstuhl, Sepp; Freitag, Johannes; Paden, John D.; Dahl-Jensen, Dorthe; Winter, Anna; Wilhelms, Frank

    2016-04-01

    A key variable when interpreting the evolution and mass loss from polar ice sheets is the input from the surface mass balance. While ice core records contain information on past accumulation rates, they always only provide information for a single location. Here, we present spatially distributed accumulation rates from central northern Greenland, specifically the area between the NEEM (North Greenland Eemian Drilling) and NGRIP (North Greenland Ice Core Project) ice core drill sites. The accumulation rates have been reconstructed using ice-penetrating radar, firn core measurements and inverse methods, and we are able to retrieve both spatial and temporal changes in the accumulation over an area spanning 300 km by 300 km. We investigate the stability of the accumulation pattern over the past several hundred years, and we address the question of how well the measured accumulation rates at the ice core sites capture the regional variations in accumulation. We find that while the accumulation rates at NEEM have been stable for the past 700 years, the NGRIP site has experienced fluctuations in accumulation rate. We interpret this as an indication of shifts in the dominating weather pattern over the ice divide in central North Greenland.

  8. A 2000-year annual record of snow accumulation rates for Law Dome, East Antarctica

    NASA Astrophysics Data System (ADS)

    Roberts, J.; Plummer, C.; Vance, T.; van Ommen, T.; Moy, A.; Poynter, S.; Treverrow, A.; Curran, M.; George, S.

    2015-05-01

    Accurate high-resolution records of snow accumulation rates in Antarctica are crucial for estimating ice sheet mass balance and subsequent sea level change. Snowfall rates at Law Dome, East Antarctica, have been linked with regional atmospheric circulation to the mid-latitudes as well as regional Antarctic snowfall. Here, we extend the length of the Law Dome accumulation record from 750 years to 2035 years, using recent annual layer dating that extends to 22 BCE. Accumulation rates were calculated as the ratio of measured to modelled layer thicknesses, multiplied by the long-term mean accumulation rate. The modelled layer thicknesses were based on a power-law vertical strain rate profile fitted to observed annual layer thickness. The periods 380-442, 727-783 and 1970-2009 CE have above-average snow accumulation rates, while 663-704, 933-975 and 1429-1468 CE were below average, and decadal-scale snow accumulation anomalies were found to be relatively common (74 events in the 2035-year record). The calculated snow accumulation rates show good correlation with atmospheric reanalysis estimates, and significant spatial correlation over a wide expanse of East Antarctica, demonstrating that the Law Dome record captures larger-scale variability across a large region of East Antarctica well beyond the immediate vicinity of the Law Dome summit. Spectral analysis reveals periodicities in the snow accumulation record which may be related to El Niño-Southern Oscillation (ENSO) and Interdecadal Pacific Oscillation (IPO) frequencies.

  9. Metal accumulation rates in northwest Atlantic pelagic sediments

    NASA Astrophysics Data System (ADS)

    Thomson, J.; Carpenter, M. S. N.; Colley, S.; Wilson, T. R. S.; Elderfield, H.; Kennedy, H.

    1984-10-01

    Measurements of 230Th, 87Sr /86Sr and twenty-four metals were made on cores from the Nares Abyssal Plain. The sediment is characterized by slowly-accumulating (0.3-0.7 g/cm 2 10 3 yr) pelagic red clays and rapidly deposited grey clays transported by turbidity currents. Despite their colour differences and the enrichment of Mn, Fe, Cu, Co, Ni, Zn, V and, to a lesser degree, the rare earths in the red clays, Sr isotope evidence demonstrates that the clays have the same terrigenous origin. The excesses of metals in the red clays have been attributed to metal removal from the water column and a comparison with the grey clays has enabled the authigenic fluxes of metals to be estimated. The fluxes obtained are in the ranges 20-50 μmol/cm 2 10 3 yr for Mn and Fe, 0.1-0.4 μmol/cm 2 10 3 yr for Cu, Co, Ni, Zn, V, Sr and Ce, 10-20 nmol/cm 2 10 3 yr for La and Nd, and 0.5-3 nmol/cm 2 10 3 yr for Sm, Eu, Gd, Dy, Er and Yb. Authigenic fluxes of Y, Nb, Cr, Zr, Rb, U and Th were not resolvable. Fluxes appear to be near constant on the Plain but comparison with other areas shows that they are quite variable both between and within ocean basins. The chief factor controlling authigenic fluxes is the geochemical abundances of the elements but fractionation within both the transition element and rare earth series can be recognized from inter-element comparisons and from differences in fluxes between Atlantic and Pacific red clays corresponding to the oceanic reactivities of the elements.

  10. Elevated rates of organic carbon, nitrogen, and phosphorus accumulation in a highly impacted mangrove wetland

    NASA Astrophysics Data System (ADS)

    Sanders, Christian J.; Eyre, Bradley D.; Santos, Isaac R.; Machado, Wilson; Luiz-Silva, Wanilson; Smoak, Joseph M.; Breithaupt, Joshua L.; Ketterer, Michael E.; Sanders, Luciana; Marotta, Humberto; Silva-Filho, Emmanoel

    2014-04-01

    The effect of nutrient enrichment on mangrove sediment accretion and carbon accumulation rates is poorly understood. Here we quantify sediment accretion through radionuclide tracers to determine organic carbon (OC), total nitrogen (TN), and total phosphorus (TP) accumulation rates during the previous 60 years in both a nutrient-enriched and a pristine mangrove forest within the same geomorphological region of southeastern Brazil. The forest receiving high nutrient loads has accumulated OC, TN, and TP at rates that are fourfold, twofold, and eightfold respectively, higher than those from the undisturbed mangrove. Organic carbon and TN stable isotopes (δ13C and δ15N) reflect an increased presence of organic matter (OM) originating with either phytoplankton, benthic algae, or another allochthonous source within the more rapidly accumulated sediments of the impacted mangrove. This suggests that the accumulation rate of OM in eutrophic mangrove systems may be enhanced through the addition of autochthonous and allochthonous nonmangrove material.

  11. Accumulator

    NASA Technical Reports Server (NTRS)

    Fenwick, J. R.; Karigan, G. H. (Inventor)

    1977-01-01

    An accumulator particularly adapted for use in controlling the pressure of a stream of fluid in its liquid phase utilizing the fluid in its gaseous phase was designed. The accumulator is characterized by a shell defining a pressure chamber having an entry throat for a liquid and adapted to be connected in contiguous relation with a selected conduit having a stream of fluid flowing through the conduit in its liquid phase. A pressure and volume stabilization tube, including an array of pressure relief perforations is projected into the chamber with the perforations disposed adjacent to the entry throat for accommodating a discharge of the fluid in either gaseous or liquid phases, while a gas inlet and liquid to gas conversion system is provided, the chamber is connected with a source of the fluid for continuously pressuring the chamber for controlling the pressure of the stream of liquid.

  12. Rates of biomass accumulation of North Carolina Piedmont forests

    SciTech Connect

    Peet, R.K.; Council, O.P.

    1980-01-01

    Recent work by Sharp et al. on the primary production of North Carolina vegetation has suggested that the average natural forest in North Carolina is unproductive relative to its potential as intensively managed forest, the difference being roughly 300%. This variation could be attributable to patterns of forest recovery after disturbance, to management practices, or even to species composition. These and other alternative hypotheses need to be tested in a careful and objective manner. The primary objectives of the present research project were twofold: (1) to use dimension analysis methods to develop a set of regression equations useful for evaluating the productivity (rate of solar energy fixation) and biomass (stored energy) of North Carolina piedmont forests; (2) to use the resulting equations to document patterns in and rates of change of forest production and biomass during forest recovery from disturbance. The first objective was divided into two component parts. First, the regression equations were developed. Second, the caloric content of trees was studied so as to allow conversion from biomass units (Kg/m/sup 2/) to energy units (Kcal/m/sup 2/). Section 2 of this report outlines the dimension analysis procedure, presents the resulting equations, and explains their use. The portion of the project assessing the caloric content of trees is presented in Section 3. The results of our second objective, the examination of changes in forest biomass and production, are presented in Section 4.

  13. Spatial variation in rates of carbon and nitrogen accumulation in a boreal bog

    SciTech Connect

    Ohlson, M.; Oekland, R.H.

    1998-12-01

    Although previous studies hint at the occurrence of substantial spatial variation in the accumulation rates of C and N in bogs, the extent to which rates may vary on high-resolution spatial and temporal scales is not known. A main reason for the lack of knowledge is that it is problematic to determine the precise age of peat at a given depth. The authors determined rates of carbon and nitrogen accumulation in the uppermost decimeters of a bog ecosystem using the pine method, which enables accurate dating of surface peat layers. They combined accumulation data with numerical and geostatistical analyses of the recent vegetation to establish the relationship between bog vegetation and rate of peat accumulation. Use of a laser technique for spatial positioning of 151 age-determined peat cores within a 20 x 20 m plot made it possible to give the first tine-scaled account of spatial and temporal variation in rates of mass, carbon, and nitrogen accumulation during the last century. Rates of C and N accumulation were highly variable at all spatial scales studied. For example, after {approximately}125 yr of peat growth, C and N accumulation varied by factors of five and four, respectively, from 25 to 125 g/dm{sup 2} for C, and from 0.7 to 2.6 g/dm{sup 2} for N. It takes 40 yr of peat accumulation before significant amounts of C are lost through decay. Hummocks built up by Sphagnum fuscum and S. rubellum were able to maintain average rates of C accumulation that exceed 2 g{center_dot}dm{sup {minus}2}{center_dot} yr{sup {minus}1} during 50 yr of growth. The authors argue that data on spatial variation in rates of C accumulation are necessary to understand the role of boreal peatlands in the greenhouse effect and global climate.

  14. Historical accumulation rates of mercury in four Scottish ombrotrophic peat bogs over the past 2000 years.

    PubMed

    Farmer, John G; Anderson, Peter; Cloy, Joanna M; Graham, Margaret C; MacKenzie, Angus B; Cook, Gordon T

    2009-10-15

    The historical accumulation rates of mercury resulting from atmospheric deposition to four Scottish ombrotrophic peat bogs, Turclossie Moss (northeast Scotland), Flanders Moss (west-central), Red Moss of Balerno (east-central) and Carsegowan Moss (southwest), were determined via analysis of (210)Pb- and (14)C-dated cores up to 2000 years old. Average pre-industrial rates of mercury accumulation of 4.5 and 3.7 microg m(-2) y(-1) were obtained for Flanders Moss (A.D. 1-1800) and Red Moss of Balerno (A.D. 800-1800), respectively. Thereafter, mercury accumulation rates increased to typical maximum values of 51, 61, 77 and 85 microg m(-2) y(-1), recorded at different times possibly reflecting local/regional influences during the first 70 years of the 20th century, at the four sites (TM, FM, RM, CM), before declining to a mean value of 27+/-15 microg m(-2) y(-1) during the late 1990s/early 2000s. Comparison of such trends for mercury with those for lead and arsenic in the cores and also with direct data for the declining UK emissions of these three elements since 1970 suggested that a substantial proportion of the mercury deposited at these sites over the past few decades originated from outwith the UK, with contributions to wet and dry deposition arising from long-range transport of mercury released by sources such as combustion of coal. Confidence in the chronological reliability of these core-derived trends in absolute and relative accumulation of mercury, at least since the 19th century, was provided by the excellent agreement between the corresponding detailed and characteristic temporal trends in the (206)Pb/(207)Pb isotopic ratio of lead in the (210)Pb-dated Turclossie Moss core and those in archival Scottish Sphagnum moss samples of known date of collection. The possibility of some longer-term loss of volatile mercury released from diagenetically altered older peat cannot, however, be excluded by the findings of this study.

  15. A two thousand year annual record of snow accumulation rates for Law Dome, East Antarctica

    NASA Astrophysics Data System (ADS)

    Roberts, J.; Plummer, C.; Vance, T.; van Ommen, T.; Moy, A.; Poynter, S.; Treverrow, A.; Curran, M.; George, S.

    2014-11-01

    Accurate high resolution records of snow accumulation rates in Antarctica are crucial for estimating ice sheet mass balance and subsequent sea level change. Snowfall rates at Law Dome, East Antarctica, have been linked with regional atmospheric circulation to mid-latitudes as well as regional Antarctic snowfall. Here, we extend the Law Dome accumulation record from 750 to 2035 years, using recent annual layer dating that extends to AD -22. Accumulation rates were calculated as the ratio of measured to modelled layer thicknesses, multiplied by the long term mean accumulation rate. The modelled layer thicknesses were based on a power law vertical strain rate profile fitted to observed annual layer thickness. The periods AD 380-442, AD 727-783 and AD 1970-2009 have above average snow accumulation rates, while AD 663-704, AD 933-975 and AD 1429-1468 were below average. The calculated snow accumulation rates show good correlation with atmospheric reanalysis estimates, and significant spatial correlation over a wide expanse of East Antarctica, demonstrating that the Law Dome record captures larger scale variability across a large region of East Antarctica well beyond the immediate vicinity of the Law Dome summit. Spectral analysis reveals periodicities in the snow accumulation record which may be related to ENSO and Interdecadal Pacific Oscillation frequencies.

  16. Comparison of elemental accumulation rates between ferromanganese deposits and sediments in the South Pacific Ocean

    USGS Publications Warehouse

    Kraemer, T.; Schornick, J.C.

    1974-01-01

    Rates of accumulation of Fe and Mn, as well as Cu, Ni, Co, Pb, Zn, Hg, U and Th have been determined for five ferromanganese deposits from four localities in the South Pacific Ocean. Manganese is accumulating in nodules and crusts at a rate roughly equivalent to that found to be accumulating in sediments in the same area. Iron shows a deficiency in accumulation in nodules and crusts with respect to sediments, especially near the continents, but also in the central and south-central Pacific. Copper is accumulating in nodules and crusts at a rate one order of magnitude less than the surrounding sediments. This is interpreted as meaning that most of the Mn is supplied as an authigenic phase to both sediments and nodules while Fe is supplied mostly by ferromanganese micro-nodules and by detrital and adsorbed components of sediments; and Cu is enriched in sediments relative to nodules and crusts most probably through biological activity. ?? 1974.

  17. Chronic Psychological Stress Induces the Accumulation of Myeloid-Derived Suppressor Cells in Mice

    PubMed Central

    Cao, Junxia; Zhang, Xueying; Zhu, Ting; Zhang, Dalin; Wang, Wendie; Wang, Jing; Shen, Beifen; Gao, Xu; Shi, Yanchun; Zhang, Jiyan

    2013-01-01

    Chronic psychological stress has been shown to adversely impact immune system functions and compromise host defenses against various infections. However, the underlying mechanisms remain elusive. Recent studies have demonstrated that myeloid-derived suppressor cells (MDSCs) play an important role in regulating immunity. It is of interest to explore whether or not chronic psychological stress plays immunosuppressive functions partially by inducing MDSCs accumulation. In this work, we report that chronic psychological stress led to the accumulation of CD11b+Gr1+ cells in the bone marrow of BALB/c mice. Repeated β-agonist infusion showed no such effect. However, β-adrenergic blockade, but not glucocorticoids blockade, partially reversed the accumulation of CD11b+Gr1+ cells under the condition of chronic psychological stress, suggesting catecholamines collaborate with other factors to induce the accumulation. Further exploration indicates that cyclooxygenase 2 (COX-2)-prostaglandin E2 (PGE2) loop might act downstream to induce the accumulation. A majority of the accumulated CD11b+Gr1+ cells were Ly6G+Ly6Clow immature neutrophils, which inhibited cytokine release of macrophages as well as T cell responsiveness. Moreover, the accumulated CD11b+Gr1+ cells under the condition of chronic psychological stress expressed multiple inhibitory molecules. Taken together, our data demonstrate for the first time that chronic psychological stress induces MDSCs accumulation in mice, which can contribute to immunosuppression. PMID:24058577

  18. Carbon and Nitrogen Accumulation Rates in Salt Marshes in Oregon, USA

    EPA Science Inventory

    Two important ecosystem services of wetlands are carbon sequestration and filtration of nutrients and particulates. We quantified the carbon and nitrogen accumulation rates in salt marshes at 135 plots distributed across eight estuaries located in Oregon, USA. Net carbon and ...

  19. Geographic variability in organic carbon stock and accumulation rate in sediments of East and Southeast Asian seagrass meadows

    NASA Astrophysics Data System (ADS)

    Miyajima, Toshihiro; Hori, Masakazu; Hamaguchi, Masami; Shimabukuro, Hiromori; Adachi, Hiroshi; Yamano, Hiroya; Nakaoka, Masahiro

    2015-04-01

    Organic carbon (OC) stored in the sediments of seagrass meadows has been considered a globally significant OC reservoir. However, the sparsity and regional bias of studies on long-term OC accumulation in coastal sediments have limited reliable estimation of the capacity of seagrass meadows as a global OC sink. We evaluated the amount and accumulation rate of OC in sediment of seagrass meadows and adjacent areas in East and Southeast Asia. In temperate sites, the average OC concentration in the top 30 cm of sediment was higher in seagrass meadows (780-1080 mmol g-1) than in sediments without seagrass cover (52-430 mmol g-1). The average OC in the top 30 cm of subtropical and tropical seagrass meadow sediments ranged from 140 to 440 mmol g-1. Carbon isotope mass balancing suggested that the contribution of seagrass-derived carbon to OC stored in sediments was often relatively minor (temperate: 10-40%; subtropical: 35-82%; tropical: 4-34%) and correlated to the habitat type, being particularly low in estuarine habitats. Stock of OC in the top meter of sediment of all the studied meadows ranged from 38 to 120 Mg ha-1. The sediment accumulation rates were estimated by radiocarbon dating of six selected cores (0.32-1.34 mm yr-1). The long-term OC accumulation rates calculated from the sediment accumulation rate and the top 30 cm average OC concentration for the seagrass meadows (24-101 kg ha-1 yr-1) were considerably lower than the OC accumulation rates previously reported for Mediterranean Posidonia oceanica meadows (580 kg ha-1 yr-1 on average). Current estimates for the global carbon sink capacity of seagrass meadows, which rely largely on Mediterranean studies, may be considerable overestimations.

  20. Marine debris accumulation in the Northwestern Hawaiian Islands: an examination of rates and processes.

    PubMed

    Dameron, Oliver J; Parke, Michael; Albins, Mark A; Brainard, Russell

    2007-04-01

    Large amounts of derelict fishing gear accumulate and cause damage to shallow coral reefs of the Northwestern Hawaiian Islands (NWHI). To facilitate maintenance of reefs cleaned during 1996-2005 removal efforts, we identify likely high-density debris areas by assessing reef characteristics (depth, benthic habitat type, and energy regime) that influence sub-regional debris accumulation. Previously cleaned backreef and lagoonal reefs at two NWHI locations were resurveyed for accumulated debris using two survey methods. Accumulated debris densities and weights were found to be greater in lagoonal reef areas. Sample weight-based debris densities are extrapolated to similar habitats throughout the NWHI using a spatial 'net habitat' dataset created by generalizing IKONOS satellite derivatives for depth and habitat classification. Prediction accuracy for this dataset is tested using historical debris point data. Annual NWHI debris accumulation is estimated to be 52.0 metric tonnes. For planning purposes, individual NWHI atolls/reefs are allotted a proportion of this total.

  1. A novel role for methyl cysteinate, a cysteine derivative, in cesium accumulation in Arabidopsis thaliana

    NASA Astrophysics Data System (ADS)

    Adams, Eri; Miyazaki, Takae; Hayaishi-Satoh, Aya; Han, Minwoo; Kusano, Miyako; Khandelia, Himanshu; Saito, Kazuki; Shin, Ryoung

    2017-02-01

    Phytoaccumulation is a technique to extract metals from soil utilising ability of plants. Cesium is a valuable metal while radioactive isotopes of cesium can be hazardous. In order to establish a more efficient phytoaccumulation system, small molecules which promote plants to accumulate cesium were investigated. Through chemical library screening, 14 chemicals were isolated as ‘cesium accumulators’ in Arabidopsis thaliana. Of those, methyl cysteinate, a derivative of cysteine, was found to function within the plant to accumulate externally supplemented cesium. Moreover, metabolite profiling demonstrated that cesium treatment increased cysteine levels in Arabidopsis. The cesium accumulation effect was not observed for other cysteine derivatives or amino acids on the cysteine metabolic pathway tested. Our results suggest that methyl cysteinate, potentially metabolised from cysteine, binds with cesium on the surface of the roots or inside plant cells and improve phytoaccumulation.

  2. A novel role for methyl cysteinate, a cysteine derivative, in cesium accumulation in Arabidopsis thaliana

    PubMed Central

    Adams, Eri; Miyazaki, Takae; Hayaishi-Satoh, Aya; Han, Minwoo; Kusano, Miyako; Khandelia, Himanshu; Saito, Kazuki; Shin, Ryoung

    2017-01-01

    Phytoaccumulation is a technique to extract metals from soil utilising ability of plants. Cesium is a valuable metal while radioactive isotopes of cesium can be hazardous. In order to establish a more efficient phytoaccumulation system, small molecules which promote plants to accumulate cesium were investigated. Through chemical library screening, 14 chemicals were isolated as ‘cesium accumulators’ in Arabidopsis thaliana. Of those, methyl cysteinate, a derivative of cysteine, was found to function within the plant to accumulate externally supplemented cesium. Moreover, metabolite profiling demonstrated that cesium treatment increased cysteine levels in Arabidopsis. The cesium accumulation effect was not observed for other cysteine derivatives or amino acids on the cysteine metabolic pathway tested. Our results suggest that methyl cysteinate, potentially metabolised from cysteine, binds with cesium on the surface of the roots or inside plant cells and improve phytoaccumulation. PMID:28230101

  3. Using transplants to measure accumulation rates of epiphytic bryophytes in forests of western Oregon

    USGS Publications Warehouse

    Rosso, A.L.; Muir, Patricia S.; Rambo, T.

    2001-01-01

    We sought a simple and effective transplant method that could be used to measure biomass accumulation rates of epiphytic bryophytes. Trials were carried out in the Pseudotsuga menziesii-dominated forests of western Oregon. We tested multiple transplant methods over a 13-month period while comparing accumulation rates of Antitrichia curtipendula (Hedw.) Brid. and Isothecium myosuroides Brid. among an old-growth stand, a young stand, and a recent clearcut. In our study area, Antitrichia is considered to be an old-growth associate while Isothecium is a more ubiquitous species. Methods tested included containment in net bags, containment in hairnets, and directly tying mats to substrates. Three sizes of transplants were tested with both natural and inert artificial substrates. Transplants of approximately five g enclosed in plastic net bags and tied to either natural or artificial substrates worked well for our purposes. Only minor differences were found in mean accumulation rates between the old growth and young stand, though variation in accumulation rates was higher in the old growth. Neither species appeared capable of surviving in the clearcut. Antitrichia accumulated biomass 60% faster in the canopy than in the understory on average. Antitrichia also accumulated at a faster rate than Isothecium, with mean 13-month biomass increases of 11.8 and 3.7% respectively for 5 g transplants in the understory. Our results suggest that Antitrichia's association with old growth may be due more to dispersal or establishment limitations than to a decreased ability to grow in young stands.

  4. Constraints on the Recent Rate of Lunar Regolith Accumulation from Diviner Observations

    NASA Technical Reports Server (NTRS)

    Ghent, R. R.; Hayne, P. O.; Bandfield, J. L.; Campbell, B. A.; Carter, L. M.

    2012-01-01

    Many large craters on the lunar nearside show radar CPR signatures consistent with the presence of blocky ejecta blankets, to distances pre dicted to be covered by continuous ejecta. However, most of these sur faces show limited enhancements in both derived rock abundance and rock-free regolith temperatures calculated from Diviner nighttime infrar ed observations. This indicates that the surface rocks are covered by a layer of thermally insulating regolith material. By matching the results of one-dimensional thermal models to Diviner nighttime temperat ures, we have constrained the thermophysical properties of the upper regolith, and the thickness of regolith overlying proximal ejecta. We find that for all of the regions surveyed (all in the nearside highla nds), the nighttime cooling curves are best fit by a density profile that varies exponentially with depth, consistent with a linear mixture of rocks and regolith fines, with increasing rock content with depth . Our results show significant spatial variations in the density e-folding depth, H, among young crater ejecta regions, indicating differen ces in the thickness of accumulated regolith. However, away from youn g craters, the average regional "equilibrium" value of H (Heq) is remarkably consistent, and is on the order of 5 cm. As expected, near-rim ejecta associated with young craters show lower values of H, indicating a high rock content in the shallow subsurface; for older craters, the average value of H approaches the regional value of Heq. Calculat ed H values for young craters show a clear correlation with published ages, providing the first observational constraint on the recent rate of lunar regolith accumulation. In addition, this result may help to resolve the apparent discrepancy between ages calculated from small crater counts on melt ponds versus counts on continuous ejecta (e.g., King crater; Ashley et al., 2011, LPSC 42, abstract 2437). This method could, in principle, be extended to other

  5. Topographic control and accumulation rate of some Holocene coral reefs: south Florida and Dry Tortugas

    USGS Publications Warehouse

    Shinn, E.A.; Hudson, J.H.; Halley, R.B.; Lidz, B.H.; Taylor, D.L.

    1977-01-01

    Core drilling and examination of underwater excavation on 6 reef sites in south Florida and Dry Tortugas revealed that underlying topography is the major factor controlling reef morphology. Carbon-14 dating on coral recovered from cores enables calculation of accumulation rates. Accumulation rates were found to range from 0.38 m/1000 years in thin Holocene reefs to as much as 4.85 m/1000 years in thicker buildups. Cementation and alteration of corals were found to be more pronounced in areas of low buildup rates than in areas of rapid accumulation rates. Acropora palmata, generally considered the major reef builder in Florida, was found to be absent in most reefs drilled. At Dry Tortugas, the more than 13-meter thick Holocene reef did not contain A. palmata. The principal reef builders in this outer reef are the same as those which built the Pleistocene Key Largo formation, long considered to be fossilized patch reef complex.

  6. Radiocarbon Dating, Chronologic Framework, and Changes in Accumulation Rates of Holocene Estuarine Sediments from Chesapeake Bay

    NASA Astrophysics Data System (ADS)

    Colman, Steven M.; Baucom, Pattie C.; Bratton, John F.; Cronin, Thomas M.; McGeehin, John P.; Willard, Debra; Zimmerman, Andrew R.; Vogt, Peter R.

    2002-01-01

    Rapidly accumulating Holocene sediments in estuaries commonly are difficult to sample and date. In Chesapeake Bay, we obtained sediment cores as much as 20 m in length and used numerous radiocarbon ages measured by accelerator mass spectrometry methods to provide the first detailed chronologies of Holocene sediment accumulation in the bay. Carbon in these sediments is a complex mixture of materials from a variety of sources. Analyses of different components of the sediments show that total organic carbon ages are largely unreliable, because much of the carbon (including coal) has been transported to the bay from upstream sources and is older than sediments in which it was deposited. Mollusk shells (clams, oysters) and foraminifera appear to give reliable results, although reworking and burrowing are potential problems. Analyses of museum specimens collected alive before atmospheric nuclear testing suggest that the standard reservoir correction for marine samples is appropriate for middle to lower Chesapeake Bay. The biogenic carbonate radiocarbon ages are compatible with 210Pb and 137Cs data and pollen stratigraphy from the same sites. Post-settlement changes in sediment transport and accumulation is an important environmental issue in many estuaries, including the Chesapeake. Our data show that large variations in sediment mass accumulation rates occur among sites. At shallow water sites, local factors seem to control changes in accumulation rates with time. Our two relatively deep-water sites in the axial channel of the bay have different long-term average accumulation rates, but the history of sediment accumulation at these sites appears to reflect overall conditions in the bay. Mass accumulation rates at the two deep-water sites rapidly increased by about fourfold coincident with widespread land clearance for agriculture in the Chesapeake watershed.

  7. Radiocarbon dating, chronologic framework, and changes in accumulation rates of holocene estuarine sediments from Chesapeake Bay

    USGS Publications Warehouse

    Colman, Steven M.; Baucom, P.C.; Bratton, J.F.; Cronin, T. M.; McGeehin, J.P.; Willard, D.; Zimmerman, A.R.; Vogt, P.R.

    2002-01-01

    Rapidly accumulating Holocene sediments in estuaries commonly are difficult to sample and date. In Chesapeake Bay, we obtained sediment cores as much as 20 m in length and used numerous radiocarbon ages measured by accelarator mass spectrometry methods to provide the first detailed chronologies of Holocene sediment accumulation in the bay. Carbon in these sediments is a complex mixture of materials from a variety of sources. Analyses of different components of the sediments show that total organic carbon ages are largely unreliable, because much of the carbon (including coal) has been transported to the bay from upstream sources and is older than sediments in which it was deposited. Mollusk shells (clams, oysters) and foraminifera appear to give reliable results, although reworking and burrowing are potential problems. Analyses of museum specimens collected alive before atmospheric nuclear testing suggest that the standard reservoir correction for marine samples is appropriate for middle to lower Chesapeake Bay. The biogenic carbonate radiocarbon ages are compatible with 210 Pb and 137 Cs data and pollen stratigraphy from the same sites. Post-settlement changes in sediment transport and accumulation is an important environmental issue in many estuaries, including the Chesapeake. Our data show that large variations in sediment mass accumulation rates occur among sites. At shallow water sites, local factors seem to control changes in accumulation rates with time. Our two relatively deep-water sites in the axial channel of the bay have different long-term average accumulation rates, but the history of sediment accumulation at these sites appears to reflect overall conditions in the bay. Mass accumulation rates at the two deep-water sites rapidly increased by about fourfold coincident with widespread land clearance for agriculture in the Chesapeake watershed.

  8. Envisat derived Elevation Changes of the Greenland Ice Sheet, and a Comparison with ICESat Results in the Accumulation Area.

    NASA Astrophysics Data System (ADS)

    Sandberg Sørensen, L.; Simonsen, S. B.; Meister, R.; Forsberg, R.; Levinsen, J. F.; Flament, T.

    2014-12-01

    We show that an along track method for deriving rates of elevation change can successfully be applied to Envisat radar altimetry data over Greenland (2002-2010). The results provide improved resolution and coverage compared to previous results obtained from cross-over methods. Also, we find that temporal changes in the elevation change rate can be derived from Envisat data, and show clear examples of this by generating five-year running means for selected areas of the Greenland ice sheet. For a period between 2003 and 2009, the elevation of the ice sheets was measured by both the laser altimeter on board ICESat and the radar altimeter on board Envisat. We compare rates of elevation change derived from ICESat and Envisat for this time span in which both sensors were operating. We focus on the area above the equilibrium line altitude, in order to specifically derive information on snow parameters. A comparison of the elevation changes observed by the two sensors shows a complex pattern, which can be explained regionally by model output describing the changes in both firn air content and accumulation rates.

  9. Impact Of Particle Agglomeration On Accumulation Rates In The Glass Discharge Riser Of HLW Melter

    SciTech Connect

    Kruger, A. A.; Rodriguez, C. A.; Matyas, J.; Owen, A. T.; Jansik, D. P.; Lang, J. B.

    2012-11-12

    The major factor limiting waste loading in continuous high-level radioactive waste (HLW) melters is an accumulation of particles in the glass discharge riser during a frequent and periodic idling of more than 20 days. An excessive accumulation can produce robust layers a few centimeters thick, which may clog the riser, preventing molten glass from being poured into canisters. Since the accumulation rate is driven by the size of particles we investigated with x-ray microtomography, scanning electron microscopy, and image analysis the impact of spinel forming components, noble metals, and alumina on the size, concentration, and spatial distribution of particles, and on the accumulation rate. Increased concentrations of Fe and Ni in the baseline glass resulted in the formation of large agglomerates that grew over the time to an average size of ~185+-155 {mu}m, and produced >3 mm thick layer after 120 h at 850 deg C. The noble metals decreased the particle size, and therefore significantly slowed down the accumulation rate. Addition of alumina resulted in the formation of a network of spinel dendrites which prevented accumulation of particles into compact layers.

  10. Character, paleoenvironment, rate of accumulation, and evidence for seismic triggering of Holocene turbidites, Canada Abyssal Plain, Arctic Ocean

    USGS Publications Warehouse

    Grantz, A.; Phillips, R.L.; Mullen, M.W.; Starratt, S.W.; Jones, Glenn A.; Naidu, A.S.; Finney, B.P.

    1996-01-01

    Four box cores and one piston core show that Holocene sedimentation on the southern Canada Abyssal Plain for the last 8010??120 yr has consisted of a continuing rain of pelagic organic and ice-rafted elastic sediment with a net accumulation rate during the late Holocene of ???10 mm/1000 yr, and episodically emplaced turbidites 1-5 m thick deposited at intervals of 830 to 3450 yr (average 2000 yr). The average net accumulation rate of the mixed sequence of turbidites and thin pelagite interbeds in the cores is about 1.2 m/1000 yr. Physiography suggests that the turbidites originated on the Mackenzie Delta or its clinoform, and ??13C values of -27 to - 25??? in the turbidites are compatible with a provenance on a delta. Extant displaced neritic and lower slope to basin plain calcareous benthic foraminifers coexist in the turbidite units. Their joint occurence indicates that the turbidites originated on the modern continental shelf and entrained sediment from the slope and rise enroute to their final resting place on the Canada Abyssal Plain. The presence of Middle Pleistocene diatoms in the turbidites suggests, in addition, that the turbidites may have originated in shallow submarine slides beneath the upper slope or outer shelf. Small but consistent differences in organic carbon content and ??13C values between the turbidite units suggest that they did not share an identical provenance, which is at least compatible with an origin in slope failures. The primary provenance of the ice-rafted component of the pelagic beds was the glaciated terrane of northwestern Canada; and the provenance of the turbidite units was Pleistocene and Holocene sedimentary deposits on the outer continental shelf and upper slope of the Mackenzie Delta. Largely local derivation of the sediment of the Canada Abyssal Plain indicates that sediment accumulation rates in the Arctic Ocean are valid only for regions with similar depositional sources and processes, and that these rates cannot be

  11. Atmospheric Pb and Ti accumulation rates from Sphagnum moss: dependence upon plant productivity.

    PubMed

    Kempter, H; Krachler, M; Shotyk, W

    2010-07-15

    The accumulation rates of atmospheric Pb and Ti were obtained using the production rates of Sphagnum mosses collected in four ombrotrophic bogs from two regions of southern Germany: Upper Bavaria (Oberbayern, OB) and the Northern Black Forest (Nordschwarzwald, NBF). Surfaces of Sphagnum carpets were marked with plastic mesh and one year later the production of plant matter was harvested. Metal concentrations were determined in acid digests using sector field ICP-MS employing well established analytical procedures. Up to 12 samples (40 x 40 cm) were collected per site, and 6-10 sites were investigated per bog. Variations within a given sampling site were in the range 2.3-4x for Pb concentrations, 1.8-2.5x for Ti concentrations, 3-8.3x for Pb/Ti, 5.6-7.8x for Pb accumulation rates, and 2.3-6.4x for Ti accumulation rates. However, the median values of these parameters for the sites (6-10 per bog) were quite consistent. The mosses from the bogs in NBF exhibited significantly greater productivity (187-202 g m(-2) a(-1)) compared to the OB peat bogs (71-91 g m(-2) a(-1)), and these differences had a pronounced effect on the Pb and Ti accumulation rates. Highly productive mosses showed no indication of a "dilution effect" of Pb or Ti concentrations, suggesting that more productive plants were simply able to accumulate more particles from the air. The median rates of net Pb accumulation by the mosses are in excellent agreement with the fluxes obtained by direct atmospheric measurements at nearby monitoring stations in both regions (EMEP and MAPESI data).

  12. Large scale spatial variation of accumulation rate across ice promontory in coastal Dronning Maud Land, Antarctica.

    NASA Astrophysics Data System (ADS)

    Callens, Denis; Matsuoka, Kenichi; Drews, Reinhard; Pattyn, Frank

    2013-04-01

    Ice rises are known to play a key role on ice shelf dynamics. By buttressing the flow, they constrain the flow of ice from the continent toward the ocean. However, since they are small hills surrounded by extremely flat area, they also play a role on atmospheric circulation. However, this impact is relatively unknown. Here, we show evidence that ice rises play a significant role on the wind redistribution of the snow. We report observations of persistent features observed all around the coast of Dronning Maud Land (DML). By analyzing radio-echo sounding data, we identified internal reflection horizons assumed to be isochronous. These layers show a remarkable variability in layer depth at both sides of the ridge, pointing to variability in surface accumulation rates. We show that a strong gradient of accumulation rate exist across, at least, 5 different ice rises in DML : Halvfarryggen Ice Rise nearby Ekstromisen (7°W), 2 ice rises into the Fimbulisen (2°E) and 2 ice rises within the Roi Baudoin Ice Shelf (25°E, Derwael & FranKenny Ice Rise). We used deepness of radar reflector as a proxy of the accumulation rate as long as we removed the influence of ice dynamics. All collected data (both low and high frequency) all show the similar persistent gradient in accumulation rate. Comparison of accumulation rate distribution and meteorological data shows that accumulation rate is twice as high on the wind side of the ridge compared to the lee side, which makes ice rise topography playing a significant role in snow redistribution. This feature is important in term of ice coring and paleoclimatic reconstruction of on time scales of 2 to 20k years.

  13. Rates and environmental controls of aeolian dust accumulation, Athabasca River Valley, Canadian Rocky Mountains

    NASA Astrophysics Data System (ADS)

    Hugenholtz, Chris H.; Wolfe, Stephen A.

    2010-09-01

    Despite an abundance of sedimentary archives of mineral dust (i.e. loess) accumulations from cold, humid environments, the absence of contemporary process investigations limits paleoenvironmental interpretations in these settings. Dust accumulations measured at Jasper Lake, a seasonally-filled reach of the glacially-fed Athabasca River in the Canadian Rocky Mountains, are some of the highest contemporary rates recorded to date. High deposition rates, including a maximum of 27,632 kg ha -1 month -1, occur during river low-flow periods, but even the lowest deposition rates, occurring during bankfull periods, exceed other contemporary rates of deposition. High rates of dust deposition may be attributed to geomorphic and climatic controls affecting sediment supply, availability and transport, and biologic factors affecting accumulation. Localized confinement of the Jasper River by tributary river alluvial fans has caused channel expansion upstream, and formation of the shallow depositional basin known as Jasper Lake. This localized sedimentary basin, coupled with large seasonal water level fluctuations and suitably high wind speeds, favors seasonal dust production. In addition, a dense source-proximal coniferous forest stand encourages high dust accumulation, via increased aerodynamic roughness and airflow deceleration. The forest stand also appears to act as an efficient dust filter, with the interception and storage of dust by the forest canopy playing a significant role with regards to secondary fallout and sediment accumulation. Overall, these results provide new insights on the environmental controls of dust entrainment and accumulation in cold, humid settings, and help clarify controls on the formation of Holocene river-sourced loess deposits.

  14. Incorporation of radiometric tracers in peat and implications for estimating accumulation rates.

    PubMed

    Hansson, Sophia V; Kaste, James M; Olid, Carolina; Bindler, Richard

    2014-09-15

    Accurate dating of peat accumulation is essential for quantitatively reconstructing past changes in atmospheric metal deposition and carbon burial. By analyzing fallout radionuclides (210)Pb, (137)Cs, (241)Am, and (7)Be, and total Pb and Hg in 5 cores from two Swedish peatlands we addressed the consequence of estimating accumulation rates due to downwashing of atmospherically supplied elements within peat. The detection of (7)Be down to 18-20 cm for some cores, and the broad vertical distribution of (241)Am without a well-defined peak, suggest some downward transport by percolating rainwater and smearing of atmospherically deposited elements in the uppermost peat layers. Application of the CRS age-depth model leads to unrealistic peat mass accumulation rates (400-600 g m(-2) yr(-1)), and inaccurate estimates of past Pb and Hg deposition rates and trends, based on comparisons to deposition monitoring data (forest moss biomonitoring and wet deposition). After applying a newly proposed IP-CRS model that assumes a potential downward transport of (210)Pb through the uppermost peat layers, recent peat accumulation rates (200-300 g m(-2) yr(-1)) comparable to published values were obtained. Furthermore, the rates and temporal trends in Pb and Hg accumulation correspond more closely to monitoring data, although some off-set is still evident. We suggest that downwashing can be successfully traced using (7)Be, and if this information is incorporated into age-depth models, better calibration of peat records with monitoring data and better quantitative estimates of peat accumulation and past deposition are possible, although more work is needed to characterize how downwashing may vary between seasons or years.

  15. The accumulation rate of meteorite falls at the earth's surface - The view from Roosevelt County, New Mexico

    NASA Technical Reports Server (NTRS)

    Zolensky, Michael E.; Wells, Gordon L.; Rendell, Helen M.

    1990-01-01

    The discovery of 154 meteorite fragments within an 11-sq km area of wind-excavated basins in Roosevelt County, New Mexico, permits a new calculation of the accumulation rate of meteorite falls at the earth's surface. Thermoluminescence dating of the coversand unit comprising the prime recovery surface suggests the maximum terrestrial age of the meteorites to be about 16.0 ka. The 68 meteorite fragments subjected to petrological analyses represent a minimum of 49 individual falls. Collection bias has largely excluded carbonaceous chondrites and achondrites, requiring the accumulation rate derived from the recovered samples to be increased by a factor of 1.25. Terrestrial weathering destroying ordinary chondrites can be modeled as a first-order decay process with an estimated half-life of 3.5 + or - 1.9 ka on the semiarid American High Plains. Having accounted for the age of the recovery surface, area of field searches, pairing of finds, collection bias and weathering half-life, an accumulation rate of 940 falls/a per 10 to the 6th sq km is calculated for falls greater than 10 g total mass. This figure exceeds the best-constrained previous estimate by more than an order of magnitude. One possible reason for this disparity may be the extraordinary length of the fall record preserved in the surficial geology of Roosevelt County. The high accumulation rate determined for the past 16 ka may point to the existence of periods when the meteorite fall rate was significantly greater than at present.

  16. Effect of accumulation rate on water stable isotopes of near-surface snow in inland Antarctica

    NASA Astrophysics Data System (ADS)

    Hoshina, Yu; Fujita, Koji; Nakazawa, Fumio; Iizuka, Yoshinori; Miyake, Takayuki; Hirabayashi, Motohiro; Kuramoto, Takayuki; Fujita, Shuji; Motoyama, Hideaki

    2014-01-01

    changes in water stable isotopes in polar firn were investigated at three sites characterized by different accumulation rates along the East Antarctic ice divide near Dome Fuji. Water stable isotopes, major ion concentrations, and tritium contents of three 2-4 m deep pits were measured at high resolution (2 cm). Temporally, the snow pits cover the past 50 years with snow accumulation rates in the range of 29-41 kg m-2 a-1 around Dome Fuji. Oxygen isotopic profiles in the three pits do not show annual fluctuations, but instead exhibit multiyear cycles. These multiyear cycles are lower in frequency at Dome Fuji as compared with the other two sites. Peaks of water stable isotopes in the multiyear cycles correspond to some ion concentration minima in the pits, although such relationships are not observed in coastal regions. We propose that the extremely low accumulation environment keeps the snow layer at the near surface, which result in postdepositional modifications of isotopic signals by processes such as ventilation and vapor condensation-sublimation. We estimate that oxygen isotopic ratios could be modified by >10‰ and that the original seasonal cycle could be completely overprinted under the accumulation conditions at Dome Fuji. Moreover, stake measurements at Dome Fuji suggest that the large variability in snow accumulation rate is the cause of the multiyear cycles.

  17. Impacts of timber harvesting on historic sediment accumulation rates in the Coos Bay estuary, Oregon

    NASA Astrophysics Data System (ADS)

    Mathabane, N.; Roering, J. J.

    2014-12-01

    The expansion and development of human infrastructure along the coastline of the Pacific Northwest has profound consequences for the habitability and general ecological health of coastal ecosystems. Coos County, one of the most economically critical regions of the Oregon Coast, experienced vigorous timber harvest activity in the aftermath of WWII that declined in the last several decades. This period of extractive land use may have drastically altered the sediment supply in the major catchments of the Coos and Millicoma Rivers and lead to variations in sediment flux into the Coos Bay estuary. Accurate sediment flux histories are critical data for deciphering the relative importance of climate and land use factors such as logging and road construction on sediment production. Reduction of root reinforcement following timber harvest increases the likelihood of shallow landsliding and debris flows. In addition, forest roads increase sediment production due to overland flow and entrainment of fine sediments on hydrologically connected roads. Although these processes have been documented in small watersheds, their compounded effect on estuaries and coastal settings has not been well documented. We use Pb-210 activities derived from sediment cores taken at various locations in the Coos Bay estuary to establish temporal variations in sediment accumulation rates (SARs). Our cores will also be analyzed to assess dissolved oxygen and other proxies for ecosystem functioning. By correlating these SARs with quantitative metrics for timber extraction rate such as board feet per year and qualitative evaluations from historical photos, we propose to document the cumulative effect of historic forest practices. The temporal resolution provided by this technique should allow us to link changes in estuarine sedimentation to changes in land use as well as climatic triggers such as storms. The conclusions of this study will add valuable information regarding the ultimate impact of

  18. In vitro Accumulation of Polyphenols in Tea Callus Derived from Anther

    PubMed Central

    Chevala, Naga Pavan Kumar; Chevala, Naga Thirumalesh; Dhanakodi, Kirubakaran; Nadendla, Rama Rao; Nagarathna, Chandrashekara Krishnappa

    2016-01-01

    Background: Tea is an economic important crop with high medicinal value due to rich polyphenols content. In the present research we studied the accumulation of polyphenols of in vitro regenerated callus from anthers. Objective: Callus induction of tea anthers and in vitro accumulation of phenolic compounds from the anther-derived callus. Materials and Methods: Standardization of callus induction for tea anthers. In vitro generated callus was screened for in vivo accumulation of catechins and its isomers were screened by FC reagent staining technique. The methanol extract of dry and green callus obtained were estimated qualitatively by Fourier transform infrared spectroscopy (FTIR)–alternative total reflection (ATR) and quantitatively by HPLC method. Results: Anthers inoculated on half strength MS media fortified with 2,4-dichloro acetic acid (2 mg/L), Kn (1 mg/L), and BAP (1 mg/L) induced callus under photoperiod of 9:15 h light. The in vivo histochemical studies revealed the accumulation of polyphenols in the callus. The in vitro generated fresh and dry callus were used for extraction and screened for accumulated polyphenols [galic acid, (+)-catechin (C), (−)-epicatechin, (−)-epigallocatechin, (−)-epigallocatechin gallate, (−)-gallocatechins, (−)-epicatechin gallate] were estimated qualitatively by FTIR–ATR method and quantitatively by HPLC method. Conclusion: The FC staining technique used here helps in localization of polyphenol compounds accumulation in the tissues by instant microscopic studies. The study have scope in large-scale isolation of various medicinally important flavonol by using anther culture. Abbreviations used: HPLC: high pressure liquid chromatography; FTIR: Fourier transform infrared spectroscopy; 2,4-D: 2,4-dichloro acetic acid; BAP: N6-benzyl amino purine; kn: kinetin PMID:27761066

  19. Holocene Carbon Accumulation Rates in the SPRUCE Bog Prior to Warming and Elevated CO2 Treatment

    NASA Astrophysics Data System (ADS)

    McFarlane, K. J.; Iversen, C. M.; Phillips, J. R.; Brice, D. J.; Hanson, P. J.

    2015-12-01

    In the Spruce and Peatland Responses Under Climatic and Environmental Change (SPRUCE) experiment warming and elevated CO2 treatments are being applied to an ombrotrophic spruce bog: the S1 Bog (S1) at Marcell Experimental Forest in northern Minnesota. To provide a historical context for recent and expected experimentally-induced changes in the bog's belowground carbon balance, we reconstructed historical carbon accumulation rates in peat using radiocarbon from 19 peat cores collected from randomly distributed SPRUCE plots. This unusually high number of cores allows us to assess spatial variability in age-depth profiles and accumulation rates across the SPRUCE study area within S1. This data, along with recent C flux measurements, show that the bog has been accumulating carbon for at least 12,0000 years and has continued to be a sink for atmospheric carbon of approximately 150 g C m-2 yr-1 in recent decades. Early Holocene accumulation rates are similar to those reported for other northern peatlands (approximately 25 g C m-2 yr-1), but apparent carbon accumulation decreased substantially around 3,000 years ago (to 5-15 g C m-2 yr-1) and stayed low until the last century. This decrease is considerably larger than that reported for other peatlands and is therefore unlikely to result only from cooling during the Holocene or bog succession. Although no charcoal has been found in peat at this site, evidence from a neighboring bog indicates a considerable amount of peat formed during this period was consumed by fire and it is possible that smoldering fires consumed peat, resulting in low apparent accumulation rates. Past droughts may have also contributed to observed trends by lowering the acrotelm/catotelm boundary, allowing for enhanced aerobic peat decomposition. This work provides important background information on spatial variability and carbon biogeochemistry that will aid in interpretation of climate change simulation experiments at S1.

  20. Nutritive Value and Herbage Accumulation Rates of Pasture Sown to Grass, Legume, and Chicory

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Planting pastures to mixtures of forages may benefit herbage production; however, wide fluctuations in botanical composition could cause unstable nutritive value. A grazing study was conducted to examine how forage mixture complexity influenced nutritive value and accumulation rate during spring, su...

  1. Distinct mutation accumulation rates among tissues determine the variation in cancer risk

    PubMed Central

    Hao, Dapeng; Wang, Li; Di, Li-jun

    2016-01-01

    Cancer is believed to be a result of accumulated mutations. However, this concept has not been fully confirmed owing to the impossibility of tracking down the ancestral somatic cell. We sought to verify the concept by exploring the correlation between cancer risk and mutation accumulation among different tissues. We hypothesized that the detected mutations through bulk tumor sequencing are commonly shared in majority, if not all, of tumor cells and are therefore largely a reflection of the mutations accumulated in the ancestral cell that gives rise to tumor. We collected a comprehensive list of mutation frequencies revealed by bulk tumor sequencing, and investigated its correlation with cancer risk to mirror the correlation between mutation accumulation and cancer risk. This revealed an approximate 1:1 relationship between mutation frequency and cancer risk in 41 different cancer types based on the sequencing data of 5,542 patients. The correlation strongly suggests that variation in cancer risk among tissues is mainly attributable to distinct mutation accumulation rates. Moreover, the correlation establishes a baseline to evaluate the effect of non-mutagenic carcinogens on cancer risk. Finally, our mathematic modeling provides a reasonable explanation to reinforce that cancer risk is predominantly determined by the first rate-limiting mutation. PMID:26785814

  2. Constraints on the recent rate of lunar regolith accumulation from Diviner observations

    NASA Astrophysics Data System (ADS)

    Ghent, R. R.; Hayne, P. O.; Bandfield, J. L.; Campbell, B. A.; Carter, L. M.; Allen, C.; Paige, D. A.

    2012-12-01

    Many large craters on the lunar nearside show radar CPR signatures consistent with the presence of blocky ejecta blankets, to distances predicted to be covered by continuous ejecta. However, most of these surfaces show limited enhancements in both derived rock abundance and rock-free regolith temperatures calculated from Diviner nighttime infrared observations. This indicates that the surface rocks are covered by a layer of thermally insulating regolith material. By matching the results of one-dimensional thermal models to Diviner nighttime temperatures, we have constrained the thermophysical properties of the upper regolith, and the thickness of regolith overlying proximal ejecta. We find that for all of the regions surveyed (all in the nearside highlands), the nighttime cooling curves are best fit by a density profile that varies exponentially with depth, consistent with a linear mixture of rocks and regolith fines, with increasing rock content with depth. Our results show significant spatial variations in the density e-folding depth, H, among young crater ejecta regions, indicating differences in the thickness of accumulated regolith. However, away from young craters, the average regional "equilibrium" value of H (Heq) is remarkably consistent, and is on the order of 5 cm. As expected, near-rim ejecta associated with young craters show lower values of H, indicating a high rock content in the shallow subsurface; for older craters, the average value of H approaches the regional value of Heq. Calculated H values for young craters (Giordano Bruno, Moore F, Byrgius A, Necho, Tycho, Jackson, King, and Copernicus) show a clear correlation with published ages, providing the first observational constraint on the recent rate of lunar regolith accumulation. In addition, this result may help to resolve the apparent discrepancy between ages calculated from small crater counts on melt ponds versus counts on continuous ejecta (e.g., King crater; Ashley et al., 2011, LPSC 42

  3. Accumulation of phenylpropanoid derivatives in chitosan-induced cell suspension culture of Cocos nucifera.

    PubMed

    Chakraborty, Moumita; Karun, Anitha; Mitra, Adinpunya

    2009-01-01

    Chitosan-induced elicitation responses of dark-incubated Cocos nucifera (coconut) endosperm cell suspension cultures led to the rapid formation of phenylpropanoid derivatives, which essentially mimics the defense-induced biochemical changes in coconut palm as observed under in vivo conditions. An enhanced accumulation of p-hydroxybenzoic acid as the major wall-bound phenolics was evident. This was followed by p-coumaric acid and ferulic acid. Along with enhanced peroxidases activities in elicited lines, the increase in activities of the early phenylpropanoid pathway enzymes such as, phenylalanine ammonia lyase (PAL), p-coumaroyl-CoA ligase (4CL) and p-hydroxybenzaldehyde dehydrogenase (HBD) in elicited cell cultures were also observed. Furthermore, supplementation of specific inhibitors of PAL, C4H and 4CL in elicited cell cultures led to suppressed accumulation of p-hydroxybenzoic acid, which opens up interesting questions regarding the probable route of the biosynthesis of this phenolic acid in C. nucifera.

  4. Atmospheric deposition of toxaphene to eastern north America derived from peat accumulation

    NASA Astrophysics Data System (ADS)

    Rapaport, R. A.; Eisenreich, S. J.

    Dated peat cores from Minnesota east to Nova Scotia analyzed for toxaphene provided spatial variation in historical and recent atmospheric fluxes and an atmospheric input function for toxaphene extending over the past 40 years. This input function is consistent with toxaphene production data and the input of a well documented insecticide, DDT. Total core burdens of toxaphene across a west to east transect were highest in the upper midwest and Nova Scotia, with recent peat accumulation rates ranging from 0.5 to 9 μg m -2a -1. Atmospheric concentrations of toxaphene, back-calculated from accumulation rates in peat, range from 8 to 150 pg m -3, in agreement with recently-measured concentrations in remote atmospheres. Recent atmospheric inputs of toxaphene are two to four times those of PCBs and DDT, respectively.

  5. Derivation of rating curve by the Tsallis entropy

    NASA Astrophysics Data System (ADS)

    Singh, Vijay P.; Cui, Huijuan; Byrd, Aaron R.

    2014-05-01

    The stage-discharge relation, often called rating curve, is employed to determine discharge in natural and engineered channels. There are several methods for deriving a rating curve most of which are empirical. It is well recognized that rating curves are subjected to significant uncertainty, yet most of these methods do not have any provision to account for or do not quantify the uncertainty. This study employs the Tsallis entropy for deriving the rating curve, based on two simple constraints: (1) total probability and (2) mean discharge. Parameters of the derived curve are determined with the use of these two constraints. The rating curve is also determined by reparameterization with the use of an entropy parameter. The Tsallis entropy permits a probabilistic characterization of the rating curve and hence the probability density function of discharge underlying the curve. It also permits a quantitative assessment of the uncertainty of discharge obtained from the rating curve. The derived rating curve is found to be in agreement with field data and is also applied to ungaged watersheds. The rating curve is also extended beyond the range of discharge values used in its construction and its validity is then evaluated.

  6. Hydroacoustic and spatial analysis of sediment fluxes and accumulation rates in two Virginia reservoirs, USA.

    PubMed

    Clark, E V; Odhiambo, B K; Yoon, S; Pilati, L

    2015-06-01

    Watershed sediment fluxes and reservoir sediment accumulation rates were analyzed in two contrasting reservoir systems in central and western Virginia. Lake Pelham, located in the Piedmont geologic province, is a human-impacted reservoir with a watershed dominated by agricultural, residential and industrial land uses. Conversely, Lake Moomaw has a largely undeveloped watershed characterized by very steep slopes and forested land use located in the Valley and Ridge province. The Revised Universal Soil Loss Equation (RUSLE) and sediment delivery ratios (SDRs) were used to estimate soil losses in the two watersheds. Bathymetric and sediment accumulation surveys of the two reservoirs were also conducted using a multi-frequency hydroacoustic surveying system. The RUSLE/SDR erosion model estimates 2150 kg ha(-1) year(-1) for Lake Pelham and 2720 kg ha(-1) year(-1) for Lake Moomaw, a 410 and 13 % increase from assumed pristine (100 % forested) land use for the respective basins. Mean sediment accumulation rates of 1.51 and 0.60 cm year(-1) were estimated from the hydroacoustic survey of Lake Pelham and Lake Moomaw, respectively. Overall, Lake Moomaw has relatively low sediment accumulation rates; however, the reservoir is vulnerable to increases in sediment fluxes with further human development due to the steep slopes and highly erodible colluvial soils that characterize the basin. Higher erosion and sediment accumulation rates in Lake Pelham are most likely reflecting the impact of human development on sedimentation processes, where the loss of vegetal buffers and increase in impervious surfaces exacerbates both the surficial soil losses as well as intrinsic stream sediment production leading to the current annual reservoir capacity loss of 0.4 %.

  7. Soil carbon stocks and their rates of accumulation and loss in a boreal forest landscape

    USGS Publications Warehouse

    Rapalee, G.; Trumbore, S.E.; Davidson, E.A.; Harden, J.W.; Veldhuis, H.

    1998-01-01

    Boreal forests and wetlands are thought to be significant carbon sinks, and they could become net C sources as the Earth warms. Most of the C of boreal forest ecosystems is stored in the moss layer and in the soil. The objective of this study was to estimate soil C stocks (including moss layers) and rates of accumulation and loss for a 733 km2 area of the BOReal Ecosystem-Atmosphere Study site in northern Manitoba, using data from smaller-scale intensive field studies. A simple process-based model developed from measurements of soil C inventories and radiocarbon was used to relate soil C storage and dynamics to soil drainage and forest stand age. Soil C stocks covary with soil drainage class, with the largest C stocks occurring in poorly drained sites. Estimated rates of soil C accumulation or loss are sensitive to the estimated decomposition constants for the large pool of deep soil C, and improved understanding of deep soil C decomposition is needed. While the upper moss layers regrow and accumulate C after fires, the deep C dynamics vary across the landscape, from a small net sink to a significant source. Estimated net soil C accumulation, averaged for the entire 733 km2 area, was 20 g C m-2 yr-1 (28 g C m-2 yr-1 accumulation in surface mosses offset by 8 g C m-2 yr-1 lost from deep C pools) in a year with no fire. Most of the C accumulated in poorly and very poorly drained soils (peatlands and wetlands). Burning of the moss layer in only 1% of uplands would offset the C stored in the remaining 99% of the area. Significant interannual variability in C storage is expected because of the irregular occurrence of fire in space and time. The effects of climate change and management on fire frequency and on decomposition of immense deep soil C stocks are key to understanding future C budgets in boreal forests.

  8. The distribution of the heavy metal accumulation rate in the biomass of three Daphnia species

    SciTech Connect

    Gajula, V.K.; Hovorka, J.; Stuchlik, E.

    1995-12-31

    The difference in the accumulation rate of a mixture of heavy metals in aquatic organisms is of considerable interest because of its importance in the prediction of the effect of pollutants in aquatic systems. In this study the authors are making an effort to evaluate the accumulation patterns of pollutants in aquatic organisms by establishing a relation between the level of an accumulated mixture of heavy metals (Cd, Zn, Pb, As, Hg) in individuals of Daphnia magna, Daphnia pulicaria and Daphnia galeata and its dry weight with respect to the form of heavy metals in the aquatic environment. One age group of Daphnia species (10 day old) were exposed to 5 ppb, 10 ppb and 20 ppb of the mixture of heavy metals for 24 hours in three different experiments. In the first experiment the mixture of heavy metals was present exclusively in labelled algae (Scendesmus actus), in the second in an aquatic medium with non labelled algae, and in the third experiment the mixture of heavy metals was dissolved in the aquatic medium only without the addition of algae. The concentration of the heavy metal mixture in individuals of D.magna; D.pulicaria and D.galeata was determined using atomic absorption spectrometry. Results were statistically evaluated and the rate of accumulation and influence of various heavy metals in the biomass of three Daphnia species is discussed.

  9. Comparison of carbon emission and accumulation rates in sub-arctic lakes

    NASA Astrophysics Data System (ADS)

    Lundin, Erik; Bastviken, David; Klaminder, Jonatan; Olid Garcia, Carolina; Karlsson, Jan

    2013-04-01

    Lakes play an important role for the carbon cycling in the sub-arctic landscape by both storing carbon in sediments and by releasing carbon to the atmosphere. Still, our knowledge regarding the importance of carbon accumulation rates vs. carbon emissions in lakes is poor, restricting large scale assessment of source sink potential of lakes in the landscape. In this study we compare annual carbon accumulation rates and CO2 and CH4 emissions to the atmosphere for six Swedish subarctic lakes. We measured the partial pressure of CO2 (every second hour) during the ice free season. CH4 emissions were measured using floating chambers. Furthermore, we sampled sediment cores from each lake (one to three cores per lake depending on lakes sizes) and calculated the recent accumulation rate of carbon into sediments by dating each core, using 210Pb. Total annual carbon emissions (CO2 + CH4) ranged between 5 to 54 g C m-2 yr-1; hence, all lakes were atmospheric net sources of carbon. Carbon emissions were overall dominated by CO2 which made up to over 90 % of the total annual carbon emission in all lakes except one, having low CO2 emission, where CH4 counted for 40% of the annual carbon emission. Sediment carbon accumulation rates were of comparable magnitudes as the emissions, counting for rates of 30 to 60% of the total carbon emission to the atmosphere. This results stress the dual role of subarctic lakes as they are acting both as atmospheric sources of CO2 and CH4 and as significant storages of carbon in sediments.

  10. Impaired phagocytosis of apoptotic cells causes accumulation of bone marrow-derived macrophages in aged mice

    PubMed Central

    Kim, Ok-Hee; Kim, Hyojung; Kang, Jinku; Yang, Dongki; Kang, Yu-Hoi; Lee, Dae Ho; Cheon, Gi Jeong; Park, Sang Chul; Oh, Byung-Chul

    2017-01-01

    Accumulation of tissue macrophages is a significant characteristic of disease-associated chronic inflammation, and facilitates the progression of disease pathology. However, the functional roles of these bone marrow-derived macrophages (BMDMs) in aging are unclear. Here, we identified age-dependent macrophage accumulation in the bone marrow, showing that aging significantly increases the number of M1 macrophages and impairs polarization of BMDMs. We found that age-related dysregulation of BMDMs is associated with abnormal overexpression of the anti-inflammatory interleukin-10. BMDM dysregulation in aging impairs the expression levels of pro-inflammatory cytokines and genes involved in B-cell maturation and activation. Phagocytosis of apoptotic Jurkat cells by BMDMs was reduced because of low expression of phagocytic receptor CD14, indicating that increased apoptotic cells may result from defective phagocytosis of apoptotic cells in the BM of aged mice. Therefore, CD14 may represent a promising target for preventing BMDM dysregulation, and macrophage accumulation may provide diagnostic and therapeutic clues. PMID:27866511

  11. Topographic heterogeneity effect on the accumulation of Fukushima-derived radiocesium on forest floor driven by biologically mediated processes

    PubMed Central

    Koarashi, Jun; Atarashi-Andoh, Mariko; Takeuchi, Erina; Nishimura, Syusaku

    2014-01-01

    The accident at the Fukushima Daiichi nuclear power plant caused serious radiocesium (137Cs) contamination of forest ecosystems located in mountainous and hilly regions with steep terrain. To understand topographic effects on the redistribution and accumulation of 137Cs on forest floor, we investigated the distribution of Fukushima-derived 137Cs in forest-floor litter layers on a steep hillslope in a Japanese deciduous forest in August 2013 (29 months after the accident). Both leaf-litter materials and litter-associated 137Cs were accumulated in large amounts at the bottom of the hillslope. At the bottom, a significant fraction (65%) of the 137Cs inventory was observed to be associated with newly shed and less degraded leaf-litter materials, with estimated mean ages of 0.5–1.5 years, added via litterfall after the accident. Newly emerged leaves were contaminated with Fukushima-derived 137Cs in May 2011 (two months after the accident) and 137Cs concentration in them decreased with time. However, the concentrations were still two orders of magnitude higher than the pre-accident level in 2013 and 2014. These observations are the first to show that 137Cs redistribution on a forested hillslope is strongly controlled by biologically mediated processes and continues to supply 137Cs to the bottom via litterfall at a reduced rate. PMID:25358420

  12. Formation and distribution of coal measure-derived hydrocarbon accumulation in NW China

    SciTech Connect

    Wenxhi Zhao; Yan Zhang; Dafeng Xu; Changyi Zhao

    1996-12-31

    This study recognizes that the following conditions are necessary for the Jurassic coal measure-derived oil and gas fields in NW China: (1) The Jurassic original basins, which were dominated by lacustrine to low-positional swamp environments, should exist to accept coal-measure related sediments, (2) the original depositional settings were characterized by low and gentle depressions and humid climate where oil-prone organic materials accumulated, (3) the development of the subsequent basins and successive sedimentation should occur on the Jurassic original basins, which are necessary to keep the coal measure source rocks progressively maturing, and, (4) a certain degree of tectonic compression took place soon after the maturation of source rocks, which provided the driving force for the effective expulsion of oil and gas from coal measures. Most of the coal measure-derived oil & gas fields in NW China are horizontally distributed along the inner side of lake strandlines. They occur vertically above or below the threshold of maturation. Owing to the sharp variations of lithology and facies in coal measures, the lithologies and hydrocarbon compositions of oil and gas reservoirs play a significant role in the oil and gas accumulations of the coal measures sequences.

  13. Formation and distribution of coal measure-derived hydrocarbon accumulation in NW China

    SciTech Connect

    Wenxhi Zhao; Yan Zhang; Dafeng Xu; Changyi Zhao )

    1996-01-01

    This study recognizes that the following conditions are necessary for the Jurassic coal measure-derived oil and gas fields in NW China: (1) The Jurassic original basins, which were dominated by lacustrine to low-positional swamp environments, should exist to accept coal-measure related sediments, (2) the original depositional settings were characterized by low and gentle depressions and humid climate where oil-prone organic materials accumulated, (3) the development of the subsequent basins and successive sedimentation should occur on the Jurassic original basins, which are necessary to keep the coal measure source rocks progressively maturing, and, (4) a certain degree of tectonic compression took place soon after the maturation of source rocks, which provided the driving force for the effective expulsion of oil and gas from coal measures. Most of the coal measure-derived oil gas fields in NW China are horizontally distributed along the inner side of lake strandlines. They occur vertically above or below the threshold of maturation. Owing to the sharp variations of lithology and facies in coal measures, the lithologies and hydrocarbon compositions of oil and gas reservoirs play a significant role in the oil and gas accumulations of the coal measures sequences.

  14. Daily accumulation rates of marine debris on sub-Antarctic island beaches.

    PubMed

    Eriksson, Cecilia; Burton, Harry; Fitch, Stuart; Schulz, Martin; van den Hoff, John

    2013-01-15

    The worlds' oceans contain a large but unknown amount of plastic debris. We made daily collections of marine debris stranded at two sub-Antarctic islands to establish (a) physical causes of strandings, and (b) a sampling protocol to better estimate the oceans' plastic loading. Accumulation rates at some beaches were dependent on tide and onshore winds. Most of the 6389 items collected were plastic (Macquarie 95%, Heard 94%) and discarded or lost fishing gear comprised 22% of those plastic items. Stalked barnacles (Lepas spp.) were a regular attachment on Macquarie debris but not at Heard Island. The daily accumulation rate of plastic debris on Macquarie Island was an order of magnitude higher than that estimated from monthly surveys during the same 4 months in the previous 5 years. This finding suggests that estimates of the oceans' plastic loading are an order of magnitude too low.

  15. Trawling-induced alterations of deep-sea sediment accumulation rates during the Anthropocene

    NASA Astrophysics Data System (ADS)

    Puig, P.; Paradis, S.; Masque, P.; Martin, J.; Juan, X.; Palanques, A.

    2015-12-01

    Commercial bottom trawling causes direct physical disturbance of the marine sedimentary environments by scraping and ploughing the seabed, generating periodic resuspension of surface sediments. However, the quantification of the sediment that is removed by trawling and exported across the continental margin remains largely unaddressed, and the preservation of the signal of such impacts in the geological record have been mostly overlooked. The analysis of sediment cores collected along the Catalan margin (NW Mediterranean) has allowed evaluating the contribution of this anthropogenic activity to the present-day sediment dynamics. Sediment cores at intensively trawled sites are characterized by over-consolidated sediments with lower 210Pb surface concentrations and inventories that indicate widespread erosion of recent sedimentary deposits. In turn, combined 210Pb and 137Cs chronologies indicate a significant increase of sediment accumulation rates within submarine canyon environments since the 1970s, coincidently with a strong impulse in the industrialization of the trawling fleets of this region. Two sampling sites that exhibited high sediment accumulation rates (0.6-0.7 cm/y) were reoccupied 1-2 decades after the first studies and revealed a second and even larger increase of sediment accumulation rates (>2 cm/y) occurring at the beginning of the XXI century. This recent change has been attributed to a preferential displacement of the trawling fleet towards fishing grounds surrounding submarine canyons and, also, to technical improvements in trawling vessels, presumably related to financial subsidies provided to the fishing sector. The alteration of sediment accumulation rates described in this continental margin may occur in many regions of the World's oceans given the wide geographical distribution of this human activity, and therefore, it could represent a potential marker of the Anthropocene in deep-sea environments.

  16. Balancing sample accumulation and DNA degradation rates to optimize noninvasive genetic sampling of sympatric carnivores.

    PubMed

    Lonsinger, Robert C; Gese, Eric M; Dempsey, Steven J; Kluever, Bryan M; Johnson, Timothy R; Waits, Lisette P

    2015-07-01

    Noninvasive genetic sampling, or noninvasive DNA sampling (NDS), can be an effective monitoring approach for elusive, wide-ranging species at low densities. However, few studies have attempted to maximize sampling efficiency. We present a model for combining sample accumulation and DNA degradation to identify the most efficient (i.e. minimal cost per successful sample) NDS temporal design for capture-recapture analyses. We use scat accumulation and faecal DNA degradation rates for two sympatric carnivores, kit fox (Vulpes macrotis) and coyote (Canis latrans) across two seasons (summer and winter) in Utah, USA, to demonstrate implementation of this approach. We estimated scat accumulation rates by clearing and surveying transects for scats. We evaluated mitochondrial (mtDNA) and nuclear (nDNA) DNA amplification success for faecal DNA samples under natural field conditions for 20 fresh scats/species/season from <1-112 days. Mean accumulation rates were nearly three times greater for coyotes (0.076 scats/km/day) than foxes (0.029 scats/km/day) across seasons. Across species and seasons, mtDNA amplification success was ≥95% through day 21. Fox nDNA amplification success was ≥70% through day 21 across seasons. Coyote nDNA success was ≥70% through day 21 in winter, but declined to <50% by day 7 in summer. We identified a common temporal sampling frame of approximately 14 days that allowed species to be monitored simultaneously, further reducing time, survey effort and costs. Our results suggest that when conducting repeated surveys for capture-recapture analyses, overall cost-efficiency for NDS may be improved with a temporal design that balances field and laboratory costs along with deposition and degradation rates.

  17. Organic Carbon, Nitrogen and Phosphorus Accumulation Rates in the Soils of the Everglades Mangrove Ecotone

    NASA Astrophysics Data System (ADS)

    Smoak, J. M.; Breithaupt, J. L.; Sanders, C. J.

    2015-12-01

    One of the fundamental questions with regard to coastal ecotones relates to their role in the transformation, transport and storage of biogeochemically important constituents and how that role may be altered by climate change. Coastal wetlands provide a range of valuable ecosystem services including sequestering organic carbon (OC) and nutrients in their soils at rates greater than terrestrial ecosystems on a per area basis. As such the Everglades mangrove ecotone, the largest contiguous mangrove forest in North America, is a biogeochemical "hotspot" at the interface of freshwater marsh and the Gulf of Mexico. Over the last one hundred years this region has been impacted by a reduction in freshwater flow and a sea-level rise (SLR) of 2.3 mm/yr which combined to cause a landward shift in the ecotone. This creates an ideal setting to examine climate induced alterations in the mangrove-ecotone biogeochemical cycle. The ability of the Everglades mangrove forest to keep pace with SLR depends largely on the rate of organic matter accumulation as that accumulation is a key contributor to accretion. However, the basic threat from SLR can be exacerbated in some areas by accelerating organic matter mineralization due to increasing salinity. The increase in salinity supplies sulfate which functions as a terminal electron acceptor that soil microbes can utilize to enhance mineralization in the brackish ecotone regions of coastal wetlands. To investigate these processes, we measured mangrove forest soil accretion, OC, N and P accumulation rates over the most recent 10, 50 and 100 year periods (via 210Pb dating) from the Gulf of Mexico to the upper freshwater reaches of the mangrove forest within Everglades National Park. Lower organic carbon accumulation rates compared to the rest of the system were found in the ecotone region most susceptible to enhanced organic matter mineralization.

  18. Radionuclide activities, geochemistry, and accumulation rates of sediments in the Gulf of Thailand

    NASA Astrophysics Data System (ADS)

    Srisuksawad, Kanitha; Porntepkasemsan, Boonsom; Nouchpramool, Sunun; Yamkate, Pathom; Carpenter, Roy; Peterson, Michael L.; Hamilton, Terry

    1997-07-01

    Downcore concentration profiles of 210Pb, U, and Th isotopes, Al, Fe, Ti, Mn and Sc were measured in sediment box cores collected at 22 stations (16-70 m water depth) covering most of the Thai zone of the Gulf of Thailand. Distributions of excess 210Pb and the detrital elements were used to study spatial variations in sedimentary processes, mineralogy, and geochemistry between different regions of the gulf. Steady-state depositional concentrations and fluxes of excess 210Pb are 3-10 times lower in Gulf of Thailand sediments than in sediments from mid-latitudes in the northern hemisphere, reflecting lower 210Pb inputs from atmospheric fallout at 6-13°N latitude and from lower production of 210Pb from 226Ra in the shallower waters of the Gulf. U and Th concentrations are approximately 2-3 times higher than those in shelf sediments from mid-latitudes of North America, consistent with a higher proportion of granitic source rocks in the Thai environment. Downcore variations in 228Th/ 232Th activity ratios and in U activities reveal that exchange of interstitial and overlying waters and their dissolved chemicals occurs down to 20 cm in 8 of 10 cores. This benthic exchange may be important in budgets of fluxes of other soluble chemicals in this shallow shelf sea. A net flux of U isotopes from overlying water into Gulf of Thailand sediments occurs, in contrast to their release from sediments of the tropical Amazon shelf. Detectable levels of 137Cs were found only in sediments near the mouth of the largest river, the Chao Phraya. The detrital elements 232Th, 230Th, Al, Ti, and Sc all show relatively uniform downcore concentration profiles. This supports a key assumption in calculations of sediment accumulation rates from downcore profiles of 210Pb activity, that steady-state depositional conditions exist and that basic sediment mineralogy and grain size does not change. 210Pb model derived mass accumulation rates vary between 270 and 490 mg/cm 2 per year in the upper Gulf

  19. The amount and accumulation rate of plastic debris on marshes and beaches on the Georgia coast.

    PubMed

    Lee, Richard F; Sanders, Dorothea P

    2015-02-15

    The amount and accumulation rate of plastic debris at 20 sites along the Georgia coast were prepared using data reported by a number of volunteer organizations. The amount of plastic debris at highly visited barrier island beaches and estuarine marshes ranged from 300 to >1000 kg. Relatively large amount of plastics (180-500 kg) were found on less visited barrier island beaches, i.e. Blackbeard, Ossabaw and Cumberland Islands. A follow up monthly or quarterly collection study was carried out on two of the sites, a barrier beach and estuarine marsh, to determine accumulation rate in 8000 m(2) areas. Accumulation rates ranged from 0.18 to 1.28 kg/30 days-8000 m(2) on the barrier island beach and from 0.6 to 1.61 kg/30 days-8000 m(2) at the estuarine marsh site. The major type of plastics, e.g. bottles, food wrappers, plastic fragments, was highly variable at different seasons and sites. The authors recommend consideration of a standardization in reporting plastic debris, with respect to quantitation of debris and sample area.

  20. Accumulation rate and mixing of shelf sediments in the Monterey Bay National Marine Sanctuary

    USGS Publications Warehouse

    Lewis, R.C.; Coale, K.H.; Edwards, B.D.; Marot, M.; Douglas, J.N.; Burton, E.J.

    2002-01-01

    The distribution of excess 210Pb in 31 sediment cores was used to determine modern (last 100 yr) mass accumulation rates and the depth of sediment mixing on the continental shelf between Pacifica and Monterey, California, USA. Apparent mass accumulation rates average 0.27 g cm-2 yr-1 and range from 0.42 g cm-2 yr-1 to 0.12 g cm-2 yr-1. Accumulation rates were highest at mid-shelf water depths (60-100 m) adjacent to major rivers and near the head of the Ascension submarine canyon. Cores from water depths of less than 65 m had low, uniform 210Pb activity profiles and sandy textures. The uppermost 5-13 cm of 15 cores had uniform 210Pb activity profiles above a region of steadily decreasing 210Pb activity. This phenomenon was attributed to sediment mixing. The thickness of this upper layer of uniform 210Pb activity decreased southward from 13 cm, west of Pacifica, to less than 5 cm, near Monterey Canyon. This southward decrease may be attributed to shallower bioturbation in the southern study area. Integrated excess 210Pb activities were generally higher where sedimentation rates were high. They were also higher with increasing distance from major rivers. Thus, sedimentation rate alone does not explain the distribution of integrated excess 210Pb in this study area. Excess 210Pb in the seafloor is controlled by other factors such as sediment texture, the atmospheric deposition rate of 210Pb, and the residence time of sediment particles in the water column. ?? 2002 Elsevier Science B.V. All rights reserved.

  1. Accumulation rates of Th-230, Pa-231, and some transition metals on the Bermuda Rise

    USGS Publications Warehouse

    Bacon, M.P.; Rosholt, J.N.

    1982-01-01

    Measurements of 238U, 234U, 230Th, 232Th, 231Pa, Mn, Fe, Co, Ni, Cu, and Zn were made on 23 samples from core GPC-5, a 29-m giant piston core from a water depth of 4583 m on the northeastern Bermuda Rise (33??41.2???N, 57??36.9???W). This area is characterized by rapid deposition of sediment transported by abyssal currents. Unsupported 230Th and 231Pa are present throughout the core but, because of large variations in the sedimentation rate, show marked departures from exponential decay with depth. The trend with depth of the 231Paex 230Thex ratio is consistent with the average accumulation rate of 36 cm/1000 y reported earlier on the basis of radiocarbon dating and CaCO3 stratigraphy. When expressed on a carbonate-free basis, concentrations of Mn, Co, Ni, Cu, Zn, 230Thex, and 231Paex all show cyclic variations positively correlated with those of CaCO3. The correlations can be explained by a model in which all of these constituents, including CaCO3, are supplied to the sediments from the water column at a constant rate. Concentration variations are controlled mainly by varying inputs of terrigenous detritus, with low inputs occurring during interglacials and high inputs during glacials. Relationships between the metal and 230Thex concentrations permit estimates of the rates at which the metals are removed to the sediment by scavenging from the water column. The results, in ??g/cm2-1000 y, are: 4300 ?? 1100 for Mn, 46 ?? 16 for Ni and 76 ?? 26 for Cu. These rates are somewhat larger than ocean-wide averages estimated by other methods, and the absolute rate of 230Th accumulation in GPC-5 averages about nine times higher than production in the overlying water column. This part of the Bermuda Rise and similar bottom-current deposits may act as important accumulators of elements scavenged from seawater. ?? 1982.

  2. Metabolically Derived Human Ventilation Rates: A Revised Approach Based Upon Oxygen Consumption Rates (Final Report, 2009)

    EPA Science Inventory

    EPA announced the availability of the final report, Metabolically Derived Human Ventilation Rates: A Revised Approach Based Upon Oxygen Consumption Rates. This report provides a revised approach for calculating an individual's ventilation rate directly from their oxygen c...

  3. Winter Insulation By Snow Accumulation in a Subarctic Treeline Ecosystem Increases Summer Carbon Cycling Rates

    NASA Astrophysics Data System (ADS)

    Parker, T.; Subke, J. A.; Wookey, P. A.

    2014-12-01

    The effect of snow accumulation on soil carbon and nutrient cycling is attracting substantial attention from researchers. We know that deeper snow accumulation caused by high stature vegetation increases winter microbial activity and therefore carbon and nitrogen flux rates. However, until now the effect of snow accumulation, by buffering winter soil temperature, on subsequent summer soil processes, has scarcely been considered. We carried out an experiment at an alpine treeline in subarctic Sweden in which soil monoliths, contained within PVC collars, were transplanted between forest (deep winter snow) and tundra heath (shallow winter snow). We measured soil CO2efflux over two growing seasons and quantified soil microbial biomass after the second winter. We showed that respiration rates of transplanted forest soil were significantly reduced compared with control collars (remaining in the forest) as a consequence of colder, but more variable, winter temperatures. We hypothesised that microbial biomass would be reduced in transplanted forests soils but found there was no difference compared to control. We therefore further hypothesised that the similarly sized microbial pool in the control is assembled differently to the transplant. We believe that the warmer winters in forests foster more active consortia of decomposer microbes as a result of different abiotic selection pressures. Using an ecosystem scale experimental approach, we have identified a mechanism that influences summer carbon cycling rates based solely on the amount of snow that accumulates the previous winter. We conclude that modification of snow depth as a consequence of changes in vegetation structure is an important mechanism influencing soil C stocks in ecosystems where snow persists for a major fraction of the year.

  4. Modern rates of glacial sediment accumulation along a 15° S-N transect in fjords from the Antarctic Peninsula to southern Chile

    NASA Astrophysics Data System (ADS)

    Boldt, Katherine V.; Nittrouer, Charles A.; Hallet, Bernard; Koppes, Michele N.; Forrest, Brittany K.; Wellner, Julia S.; Anderson, John B.

    2013-12-01

    of glacial erosion in temperate climates rank among the highest worldwide, and the sedimentary products of such erosion record climatic and tectonic signals in many glaciated settings, as well as temporal changes in glacier behavior. Glacial sediment yields are expected to decrease with increasing latitude because decreased temperature and meltwater production reduce glacial sliding, erosion, and sediment transfer; however, this expectation lacks a solid supportive database. Herein we present modern 210Pb-derived sediment accumulation rates on decadal to century time scales for 12 fjords spanning 15° of latitude from the Antarctic Peninsula to southern Chile and interpret the results in light of glacimarine sediment accumulation worldwide. 210Pb records from the Antarctic Peninsula show surprisingly steady sediment accumulation throughout the past century at rates of 1-7 mm yr-1, despite rapid warming and glacial retreat. Cores from the South Shetland Islands reveal accelerated sediment accumulation over the past few decades, likely due to changes in the thermal state of the glaciers in this region, which straddles the boundary between subpolar and temperate conditions. In Patagonia and Tierra del Fuego, sediment accumulates faster (11-24 mm yr-1), and previously collected seismic profiles show that rates reach meters per year close to the glacier termini. This increase in sediment accumulation rates with decreasing latitude reflects the gradient from subpolar to temperate climates and is consistent with glacial erosion being much faster in the temperate climate of southern Chile than in the polar climate of the Antarctic Peninsula.

  5. Systems Level Regulation of Rhythmic Growth Rate and Biomass Accumulation in Grasses

    SciTech Connect

    Kay, Steve A.

    2013-05-02

    Several breakthroughs have been recently made in our understanding of plant growth and biomass accumulation. It was found that plant growth is rhythmically controlled throughout the day by the circadian clock through a complex interplay of light and phytohormone signaling pathways. While plants such as the C4 energy crop sorghum (Sorghum bicolor (L.) Moench) and possibly the C3 grass (Brachypodium distachyon) also exhibit daily rhythms in growth rate, the molecular details of its regulation remain to be explored. A better understanding of diurnally regulated growth behavior in grasses may lead to species-specific mechanisms highly relevant to future strategies to optimize energy crop biomass yield. Here we propose to devise a systems approach to identify, in parallel, regulatory hubs associated with rhythmic growth in C3 and C4 plants. We propose to use rhythmicity in daily growth patterns to drive the discovery of regulatory network modules controlling biomass accumulation.

  6. Differential accumulation of monolignol-derived compounds in elicited flax (Linum usitatissimum) cell suspension cultures.

    PubMed

    Hano, C; Addi, M; Bensaddek, L; Crônier, D; Baltora-Rosset, S; Doussot, J; Maury, S; Mesnard, F; Chabbert, B; Hawkins, S; Lainé, E; Lamblin, F

    2006-04-01

    Lignin and lignans share monolignols as common precursors and are both potentially involved in plant defence against pathogens. In this study, we investigated the effects of fungal elicitors on lignin and lignan metabolism in flax (Linum usitatissimum) cell suspensions. Cell suspension cultures of flax were treated with elicitor preparations made from mycelium extracts of Botrytis cinerea, Phoma exigua and Fusarium oxysporum F ssp lini. Elicitors induced a rapid stimulation of the monolignol pathway, as confirmed by the increase in PAL (phenylalanine ammonia-lyase, EC 4.1.3.5), CCR (cinnamoyl-CoA reductase EC 1.2.1.44) and CAD (cinnamyl alcohol dehydrogenase EC 1.1.1.195) gene expression and PAL activity. At the same time, CCR activity only increased significantly in F. oxysporum-treated cells 24 h post elicitation. On the other hand, CAD activity measured for coniferyl alcohol formation was transiently decreased but a substrate-specific activation of CAD activity was observed in F. oxysporum-treated cells when using sinapyl alcohol as substrate. The accumulation of monolignol-derived products varied according to the elicitor used. B. cinerea or P. exigua-elicited cell cultures were characterised by a reinforcement of the cell wall by a deposit of 8-O-4'-linked non-condensed lignin structures and phenolic monomers, while at the same time no stimulation of 8-8'-linked lignan or 8-5'-linked phenylcoumaran lignan accumulation was observed. Additionally, elicitation of cell cultures with F. oxysporum extracts even triggered a strong incorporation of monolignols in the non condensed labile ether-linked lignin fraction concomitantly with a decrease in lignan and phenylcoumaran lignan accumulation. Several hypotheses are proposed to explain the putative role of these compounds in the defence response of flax cells against pathogens.

  7. Proinflammatory S100 proteins regulate the accumulation of myeloid-derived suppressor cells.

    PubMed

    Sinha, Pratima; Okoro, Chinonyerem; Foell, Dirk; Freeze, Hudson H; Ostrand-Rosenberg, Suzanne; Srikrishna, Geetha

    2008-10-01

    Chronic inflammation is a complex process that promotes carcinogenesis and tumor progression; however, the mechanisms by which specific inflammatory mediators contribute to tumor growth remain unclear. We and others recently demonstrated that the inflammatory mediators IL-1beta, IL-6, and PGE(2) induce accumulation of myeloid-derived suppressor cells (MDSC) in tumor-bearing individuals. MDSC impair tumor immunity and thereby facilitate carcinogenesis and tumor progression by inhibiting T and NK cell activation, and by polarizing immunity toward a tumor-promoting type 2 phenotype. We now show that this population of immature myeloid cells induced by a given tumor share a common phenotype regardless of their in vivo location (bone marrow, spleen, blood, or tumor site), and that Gr1(high)CD11b(high)F4/80(-)CD80(+)IL4Ralpha(+/-)Arginase(+) MDSC are induced by the proinflammatory proteins S100A8/A9. S100A8/A9 proteins bind to carboxylated N-glycans expressed on the receptor for advanced glycation end-products and other cell surface glycoprotein receptors on MDSC, signal through the NF-kappaB pathway, and promote MDSC migration. MDSC also synthesize and secrete S100A8/A9 proteins that accumulate in the serum of tumor-bearing mice, and in vivo blocking of S100A8/A9 binding to MDSC using an anti-carboxylated glycan Ab reduces MDSC levels in blood and secondary lymphoid organs in mice with metastatic disease. Therefore, the S100 family of inflammatory mediators serves as an autocrine feedback loop that sustains accumulation of MDSC. Since S100A8/A9 activation of MDSC is through the NF-kappaB signaling pathway, drugs that target this pathway may reduce MDSC levels and be useful therapeutic agents in conjunction with active immunotherapy in cancer patients.

  8. LATE CENOZOIC INCREASE IN ACCUMULATION RATES OF TERRESTRIAL SEDIMENT: How Might Climate Change Have Affected Erosion Rates?

    NASA Astrophysics Data System (ADS)

    Molnar, Peter

    2004-05-01

    Accumulation rates of terrestrial sediment have increased in the past few million years both on and adjacent to continents, although not everywhere. Apparently, erosion has increased in elevated terrain regardless of when last tectonically active or what the present-day climate. In many regions, sediment coarsened abruptly in late Pliocene time. Sparser data suggest increased sedimentation rates at 15 Ma, approximately when oxygen isotopes in benthic foraminifera imply high-latitude cooling. If climate change effected accelerated erosion, understanding how it did so remains the challenge. Some obvious candidates, such as lowered sea level leading to erosion of continental shelves or increased glaciation, account for increased sedimentation in some, but not all, areas. Perhaps stable climates that varied slowly allowed geomorphic processes to maintain a state of equilibrium with little erosion until 34 Ma, when large oscillations in climate with periods of 20,00040,000 years developed and denied the landscape the chance to reach equilibrium.

  9. Derivation of instanton rate theory from first principles

    NASA Astrophysics Data System (ADS)

    Richardson, Jeremy O.

    2016-03-01

    Instanton rate theory is used to study tunneling events in a wide range of systems including low-temperature chemical reactions. Despite many successful applications, the method has never been obtained from first principles, relying instead on the "Im F" premise. In this paper, the same expression for the rate of barrier penetration at finite temperature is rederived from quantum scattering theory [W. H. Miller, S. D. Schwartz, and J. W. Tromp, J. Chem. Phys. 79, 4889 (1983)] using a semiclassical Green's function formalism. This justifies the instanton approach and provides a route to deriving the rate of other processes.

  10. Moment accumulation rate on faults in California inferred from viscoelastic earthquake cycle models (Invited)

    NASA Astrophysics Data System (ADS)

    Johnson, K. M.

    2009-12-01

    Calculations of moment accumulation rates on active faults require knowledge of long-term fault slip rates and the area of the fault that is locked interseismically. These parameters are routinely estimated from geodetic data using elastic block models with back slip on dislocations in an elastic half-space. Yet, the elastic models are inconsistent with studies that infer postseismic viscous flow in the lower crust and mantle occurring for decades following large earthquakes. Viscous flow in the lower crust and mantle generates rapid, localized deformation early in the earthquake cycle and slower, more diffuse deformation later in the cycle. Elastic models which neglect this time-dependent flow process may lead to biased estimates of fault slip rates and locking distribution. To address this issue we have developed a three-dimensional earthquake cycle model consisting of fault-bounded blocks in an elastic crust overlying a viscoelastic lower crust and uppermost mantle. It is a kinematic model in which long-term motions of fault-bounded blocks is imposed. Interseismic locking of faults and associated deformation is modeled with steady back-slip on faults and imposed periodic earthquakes. Creep on unlocked portions of the faults occurs at constant stress and therefore the instantaneous creep rate is proportional to the instantaneous stressing rate on the fault. We compare geologic slip rate estimates in southern California with model estimates using GPS data and show that elastic block models underpredict slip rates on several faults that are late in the earthquake cycle and overpredict slip rates on faults that are early in the earthquake cycle. The viscoelastic cycle model, constrained by earthquake timing from the geologic record, predicts fault slip rates that are entirely consistent with geologic estimates for all major faults in southern California. For northern California, fault slip rate estimates using geodetic data appear not to be strongly dependent on

  11. Inferring time derivatives including cell growth rates using Gaussian processes

    PubMed Central

    Swain, Peter S.; Stevenson, Keiran; Leary, Allen; Montano-Gutierrez, Luis F.; Clark, Ivan B.N.; Vogel, Jackie; Pilizota, Teuta

    2016-01-01

    Often the time derivative of a measured variable is of as much interest as the variable itself. For a growing population of biological cells, for example, the population's growth rate is typically more important than its size. Here we introduce a non-parametric method to infer first and second time derivatives as a function of time from time-series data. Our approach is based on Gaussian processes and applies to a wide range of data. In tests, the method is at least as accurate as others, but has several advantages: it estimates errors both in the inference and in any summary statistics, such as lag times, and allows interpolation with the corresponding error estimation. As illustrations, we infer growth rates of microbial cells, the rate of assembly of an amyloid fibril and both the speed and acceleration of two separating spindle pole bodies. Our algorithm should thus be broadly applicable. PMID:27941811

  12. Inferring time derivatives including cell growth rates using Gaussian processes

    NASA Astrophysics Data System (ADS)

    Swain, Peter S.; Stevenson, Keiran; Leary, Allen; Montano-Gutierrez, Luis F.; Clark, Ivan B. N.; Vogel, Jackie; Pilizota, Teuta

    2016-12-01

    Often the time derivative of a measured variable is of as much interest as the variable itself. For a growing population of biological cells, for example, the population's growth rate is typically more important than its size. Here we introduce a non-parametric method to infer first and second time derivatives as a function of time from time-series data. Our approach is based on Gaussian processes and applies to a wide range of data. In tests, the method is at least as accurate as others, but has several advantages: it estimates errors both in the inference and in any summary statistics, such as lag times, and allows interpolation with the corresponding error estimation. As illustrations, we infer growth rates of microbial cells, the rate of assembly of an amyloid fibril and both the speed and acceleration of two separating spindle pole bodies. Our algorithm should thus be broadly applicable.

  13. Landslide Susceptibility Zonation through ratings derived from Artificial Neural Network

    NASA Astrophysics Data System (ADS)

    Chauhan, Shivani; Sharma, Mukta; Arora, M. K.; Gupta, N. K.

    2010-10-01

    In the present study, Artificial Neural Network (ANN) has been implemented to derive ratings of categories of causative factors, which are then integrated to produce a landslide susceptibility zonation map in an objective manner. The results have been evaluated with an ANN based black box approach for Landslide Susceptibility Zonation (LSZ) proposed earlier by the authors. Seven causative factors, namely, slope, slope aspect, relative relief, lithology, structural features (e.g., thrusts and faults), landuse landcover, and drainage density, were placed in 42 categories for which ratings were determined. The results indicate that LSZ map based on ratings derived from ANN performs exceedingly better than that produced from the earlier ANN based approach. The landslide density analysis clearly showed that susceptibility zones were in close agreement with actual landslide areas in the field.

  14. Constraining mass accumulation rates across the Cretaceous-Paleogene boundary clay layer using extraterrestrial helium-3

    NASA Astrophysics Data System (ADS)

    Giron, M.; Sepulveda, J.; Mukhopadhyay, S.; Alegret, L.; Summons, R. E.

    2012-12-01

    The extended duration of the negative δ13C excursion observed in marine carbonates spanning the Cretaceous-Paleogene (K-Pg) mass extinction event has lead to two main hypothesized post-extinction models ("Strangelove" and "Living Ocean";[1, 2]) for the status of marine primary productivity and the global carbon cycle. However, these models are largely inconsistent with recent paleontological and geochemical evidence suggesting heterogeneous changes in marine productivity and carbon export [3, 4]. While the analysis of lipid biomarkers in the cosmopolitan boundary clay layer allows us to assess changes in primary production by non-calcifying organisms in the immediate aftermath of the mass extinction [4], our poor understanding of the deposition of the clay layer precludes a more detailed reconstruction of short-term variations in marine ecosystem resilience. Here, we present data on extraterrestrial 3He derived from interplanetary dust particles used as a constant flux proxy to constrain fluctuations in mass accumulation rates (MARs) [5] and the duration of the boundary clay deposition in three classic and expanded K-Pg boundary sections: El Kef (Tunisia), Caravaca (Spain), and Kulstirenden (Denmark). Our results from different depositional environments indicate average durations for the sedimentation of the clay layer that are comparable (~10 kyr) to other localities [5], thus confirming its globally brief deposition. Early Paleogene MARs vary among locations when compared to background Late Cretaceous values and do not strictly follow carbonate content as traditionally assumed, thus suggesting variable depositional conditions at different locations. Changes in sediment MARs across the K-Pg will be used to calculate MARs of algal- and bacterial-derived biomarkers, as well as benthic foraminifera, in order to assess the timing and global nature of the recovery of marine primary production and carbon export. 1. Hsu, K.J., He, Q., Mckenzie, J.A., Weissert, H

  15. Holocene sediment accumulation rates in fjords and bays of Chilean Patagonia and the Antarctic Peninsula

    NASA Astrophysics Data System (ADS)

    Wellner, J. S.; Anderson, J. B.; Milliken, K.; Fernandez, R.; Michalchuk, B.; Boyd, B.

    2007-12-01

    Beginning in 2005 and ending in May of 2007, we completed a series of four research cruises in the fjords and bays of Chilean Patagonia and the Antarctic Peninsula aimed, in part, at determining the style and rate of Holocene sediment accumulation in a range of glacial settings. Our original hypothesis stated that rates of glacial erosion are a function of sliding speed, and are therefore expected to diminish sharply as basal temperatures drop below the melting point. To test this hypothesis, we measured sediment accumulation in tidewater glacier fjords ranging from fast-moving temperate glaciers in Patagonia to slower moving polar glaciers on the Antarctic Peninsula. Four fjords were surveyed in Patagonia ranging from San Rafael fjord in the Northern Patagonia ice field to Marinelli fjord in Tierra del Fuego. The cruises to the Antarctic Peninsula included two SHALDRIL legs during which drill cores recovered 108 m and 80 m of Holocene sediment in Maxwell Bay, South Shetland Islands, and the Firth of Tay in the northwestern Weddell Sea, respectively. An additional nine fjords across the northern peninsula were surveyed with kasten and jumbo piston cores. To date, we have completed nearly 100 radiocarbon dates from fossil carbonate material, both shells and foraminifera, extracted from the sediment cores obtained in these fjords. Our initial results highlight the complexity of the controls on sediment yields and the extreme variability in sediment accumulation amongst fjords. While climate may be the first order control on glacier erosion rates and sediment transport to bays and fjords, several other factors must significantly influence these processes and may mask the broader signal. Our ongoing work with this newly acquired comprehensive dataset is examining additional controls including drainage basin size, precipitation gradient, altitude of the glaciers, and glacial substrate.

  16. Unprecedented last-glacial mass accumulation rates determined by luminescence dating of loess from western Nebraska

    USGS Publications Warehouse

    Roberts, H.M.; Muhs, D.R.; Wintle, A.G.; Duller, G.A.T.; Bettis, E. Arthur

    2003-01-01

    A high-resolution chronology for Peoria (last glacial period) Loess from three sites in Nebraska, midcontinental North America, is determined by applying optically stimulated luminescence (OSL) dating to 35-50 ??m quartz. At Bignell Hill, Nebraska, an OSL age of 25,000 yr near the contact of Peoria Loess with the underlying Gilman Canyon Formation shows that dust accumulation occurred early during the last glacial maximum (LGM), whereas at Devil's Den and Eustis, Nebraska, basal OSL ages are significantly younger (18,000 and 21,000 yr, respectively). At all three localities, dust accumulation ended at some time after 14,000 yr ago. Mass accumulation rates (MARs) for western Nebraska, calculated using the OSL ages, are extremely high from 18,000 to 14,000 yr-much higher than those calculated for any other pre-Holocene location worldwide. These unprecedented MARs coincide with the timing of a mismatch between paleoenvironmental evidence from central North America, and the paleoclimate simulations from atmospheric global circulation models (AGCMs). We infer that the high atmospheric dust loading implied by these MARs may have played an important role, through radiative forcing, in maintaining a colder-than-present climate over central North America for several thousand years after summer insolation exceeded present-day values. ?? 2003 Elsevier Science (USA). All rights reserved.

  17. Rapid Changes on Sediment Accumulation Rates within Submarine Canyons Caused By Bottom Trawling Activities

    NASA Astrophysics Data System (ADS)

    Puig, P.; Masque, P.; Martin, J.; Paradis, S.; Juan, X.; Toro, M.; Palanques, A.

    2014-12-01

    The physical disturbance of the marine sedimentary environments by commercial bottom trawling is a matter of concern. The direct physical effects of this fishing technique include scraping and ploughing of the seabed and increases of the near-bottom water turbidity by sediment resuspension. However, the quantification of the sediment that has been resuspended by this anthropogenic activity over years and has been ultimately exported across the margin remains largely unaddressed. The analysis of sediment accumulation rates from sediment cores collected along the axes of several submarine canyons in the Catalan margin (northwestern Mediterranean) has allowed to estimate the contribution of this anthropogenic activity to the present-day sediment dynamics. 210Pb chronologies, occasionally supported by 137Cs dating, indicate a rapid increase of sediment accumulation rates since the 1970s, in coincidence with a strong impulse in the industrialization of the trawling fleets of this region. Such increase has been associated to the enhanced delivery of sediment resuspended by trawlers from the shelves and upper slope regions towards the canyon's interior, and to the rapid technical development at that time, in terms of engine power and gear size. This change has been observed in La Fonera (or Palamós) Canyon at depths greater than 1700 m, while in other canyons it is restricted to shallower regions (~1000 m in depth) closer to fishing grounds. Two sampling sites from La Fonera and Foix submarine canyons that exhibited high sediment accumulation rates (0.6-0.7 cm/y) were reoccupied several years after the first chronological analyses. These two new cores reveal a second and more rapid increase of sediment accumulation rates in both canyons occurring circa 2002 and accounting for about 2 cm/y. This second change at the beginning of the XXI century has been attributed to a preferential displacement of the trawling fleet towards slope fishing grounds surrounding submarine

  18. Ages and Accumulation Rates of the Martian Polar Layered Deposits Estimated from Orbital Tuning

    NASA Astrophysics Data System (ADS)

    Sori, M.; Bailey, E. A.; Perron, J.; Huybers, P. J.; Aharonson, O.; Limaye, A.

    2013-12-01

    Layers of dusty water ice in the polar caps of Mars have been hypothesized to record climate changes driven by variation of the planet's orbit and spin axis, but the time interval over which the polar layered deposits (PLDs) formed is unknown, and an orbital influence has not been conclusively demonstrated. We performed orbital tuning of reconstructed PLD stratigraphic sequences in an attempt to constrain the accumulation interval and test for the presence of an orbital signal. Our procedure uses dynamic time warping (DTW) to search for a match between two time series - the polar insolation history and brightness or topographic information in the PLDs - and then assesses the significance of potential matches using a Monte Carlo procedure. We selected 30 images of the northern PLDs from the Mars Orbiter Camera (MOC) aboard the Mars Global Surveyor spacecraft and used Mars Orbiter Laser Altimeter profiles to transform each image into a record of image brightness as a function of vertical depth. To constrain the PLD age and accumulation rate, we tuned each image record to Martian insolation records for varying time intervals. If a particular insolation interval produced the strongest match to an image, and if the match became weaker as the image was tuned to progressively longer or shorter intervals, we chose the best-fitting interval as an estimated accumulation time for that PLD sequence, and used the depth range to estimate a corresponding PLD accumulation rate. We also tuned the insolation records to synthetic records containing no orbital influence to test whether the image matches were spurious. Of the 30 MOC images analyzed, 16 produce insolation intervals that we consider strong matches. These images yield an average deposition rate of 0.5 × 0.2 mm/yr for the northern PLDs. The images represent only a fraction of the entire stratigraphy; extrapolating that deposition rate farther back in time yields an age of ~4 Ma for the entire PLD sequence present in the

  19. Field studies using the oyster Crassostrea virginica to determine mercury accumulation and depuration rates

    SciTech Connect

    Palmer, S.J.; Presley, B.J.; Powell, E.N. ); Taylor, R.J. )

    1993-09-01

    Mercury as an environmental hazard, especially with regard to human health, has been of concern since the Minamata disaster. From 1966 to 1970 a chlor-alkali plant in Point Comfort, Texas released mercury-enriched wastewater (up to 29.9 kgHg/day) into Lavaca Bay (TWQB 1977). Since 1970 the Texas Department of Health (TDH) has periodically closed and then re-opened portions of Lavaca Bay to the harvesting of crabs and finfish based on their levels (<>0.5 ppm Hg wet weight) of mercury. A 1988 closure remains in effect as of this writing. Mercury contamination in Lavaca Bay organisms thus continues to be a problem 22 years after the chlor-alkali plant ceased releasing mercury into the bay. The goal of the following research was to better understand the behavior of mercury in Lavaca Bay. Oysters have been widely used as indicator species in metal pollution studies. Most such programs have focused on the concentrations of metals in oysters from different geographic areas. This study, however, investigated the rate and amount of mercury a [open quotes]clean[close quotes] oyster would accumulate when transplanted to a contaminated estuary and the rate of mercury depuration by contaminated oysters placed in a clean environment. The oysters were additionally analyzed for Ba, Cu, Fe, P, and Zn to test for the possible involvement of these metals in mercury accumulation and depuration. 17 refs., 3 figs., 2 tabs.

  20. Distributions and accumulation rates of polycyclic aromatic hydrocarbons in the northern Gulf of Mexico sediments.

    PubMed

    Adhikari, Puspa L; Maiti, Kanchan; Overton, Edward B; Rosenheim, Brad E; Marx, Brian D

    2016-05-01

    Sediment samples collected from shelf, slope and interior basin of the northern Gulf of Mexico during 2011-2013, 1-3 years after the Deepwater Horizon (DWH) oil spill, were utilized to characterize PAH pollution history, in this region. Results indicate that the concentrations of surface ΣPAH43 and their accumulation rates vary between 44 and 160 ng g(-1) and 6-55 ng cm(-2) y(-1), respectively. ΣPAH43 concentration profiles, accumulation rates and Δ(14)C values are significantly altered only for the sediments in the immediate vicinity of the DWH wellhead. This shows that the impact of DWH oil input on deep-sea sediments was generally limited to the area close to the spill site. Further, the PAHs source diagnostic analyses suggest a noticeable change in PAHs composition from higher to lower molecular weight dominance which reflects a change in source of PAHs in the past three years, back to the background composition. Results indicate low to moderate levels of PAH pollution in this region at present, which are unlikely to cause adverse effects on benthic communities.

  1. Accumulation of cAMP augments dynamic vagal control of heart rate.

    PubMed

    Nakahara, T; Kawada, T; Sugimachi, M; Miyano, H; Sato, T; Shishido, T; Yoshimura, R; Miyashita, H; Inagaki, M; Alexander, J; Sunagawa, K

    1998-08-01

    Recent investigations in our laboratory using a Gaussian white noise perturbation technique have shown that simultaneous sympathetic stimulation augmented the gain of the transfer function from vagal stimulation frequency to heart rate response. However, the mechanism of that augmentation remains to be elucidated. In this study, we examined in anesthetized rabbits how three pharmacological interventions known to cause intracellular accumulation of cAMP affected the transfer function. Isoproterenol (0.3 microg . kg-1 . min-1 iv) increased the dynamic gain of transfer function from 7.12 +/- 0.67 to 12.4 +/- 1.21 beats . min-1 . Hz-1 (P < 0.05) without changing the corner frequency or the lag time. Similar augmentations were observed when forskolin (5 microg . kg-1 . min-1 iv) or theophylline (20 mg/kg iv) was administered under conditions of beta-adrenergic blockade. These results suggest that the accumulation of cAMP at postjunctional effector sites contributes, at least in part, to the sympathetic augmentation of the dynamic vagal control of heart rate.

  2. Carbon accumulation rates and the origin of the Holocene sapropel in the Black Sea

    NASA Astrophysics Data System (ADS)

    Calvert, S. E.; Vogel, J. S.; Southon, J. R.

    1987-10-01

    A detailed radiocarbon chronology obtained by accelerator mass spectrometry together with organic carbon and carbonate measurements on two Black Sea cores has been used to compare and contrast the burial fluxes of organic carbon in the Holocene sapropel and the modern sediment. At both deep-water and eastern-slope sites, the sapropel is separated from the modern facies by a variable thickness of compositionally homogeneous sediment with low levels of organic carbon and anomalously old radiocarbon ages. This homogeneous unit probably represents deposition by slumping or mudflow. The age limits of the sapropel are 1600 6600 B.P. at the deep-water site and 4000 6000 B.P. (radiocarbon years before 1950 A.D.) at the shallow-water site. The carbon accumulation rate in the deep-water sapropel is higher than that in the modern deep-water facies by a factor of 2 and is approximately the same as that in the modern sediment in shallow water. The revised chronology of sapropel formation and the differences in the carbon accumulation rates probably indicate that the sapropel was formed by increased production during the transition from the premodern lake to the modern marine phases of the Black Sea. This conclusion is consistent with the clear marine carbon-isotope signal in the organic matter in the sapropel in both cores (results to be reported elsewhere), in contrast to the mixed source of carbon in the other facies.

  3. A PDE Pricing Framework for Cross-Currency Interest Rate Derivatives with Target Redemption Features

    NASA Astrophysics Data System (ADS)

    Christara, Christina C.; Minh Dang, Duy; Jackson, Kenneth R.; Lakhany, Asif

    2010-09-01

    We propose a general framework for efficient pricing via a partial differential equation (PDE) approach for exotic cross-currency interest rate (IR) derivatives, with strong emphasis on long-dated foreign exchange (FX) IR hybrids, namely Power Reverse Dual Currency (PRDC) swaps with a FX Target Redemption (FX-TARN) provision. The FX-TARN provision provides a cap on the FX-linked PRDC coupon amounts, and once the accumulated coupon amount reaches this cap, the underlying PRDC swap terminates. Our PDE pricing framework is based on an auxiliary state variable to keep track of the total accumulated PRDC coupon amount. Finite differences on uniform grids and the Alternating Direction Implicit (ADI) method are used for the spatial and time discretizations, respectively, of the model-dependent PDE corresponding to each discretized value of the auxiliary variable. Numerical examples illustrating the convergence properties of the numerical methods are provided.

  4. Complex Wind-Induced Variations of Surface Snow Accumulation Rates over East Antarctica

    NASA Astrophysics Data System (ADS)

    Das, I.; Scambos, T. A.; Koenig, L.; van den Broeke, M.; Lenaerts, J.

    2015-12-01

    Accurate quantification of surface snow-accumulation over Antarctica is important for mass balance estimates and climate studies based on ice core records. Using airborne radar, lidar and thresholds of surface slope, modeled surface mass balance (SMB) and wind fields, we have predicted continent-wide distribution of wind-scour zones over Antarctica. These zones are located over relatively steep ice surfaces formed by ice flow over bedrock topography. Near-surface winds accelerate over these steeper slopes and erode and sublimate the snow. This results in numerous localized regions (typically ≤ 200 km2) with reduced or negative surface accumulation. Although small zones of re-deposition occur at the base of the steeper slope areas, the redeposited mass is small relative to the ablation loss. Total losses from wind-scour and wind-glaze areas amounts to tens of gigatons annually. Near the coast, winds often blow significant amounts of surface snow from these zones into the ocean. Large uncertainties remain in SMB estimates over East Antarctica as climate models do not adequately represent the small-scale physical processes that lead to mass loss or redistribution over the wind-scour zones. In this study, we also use Operation IceBridge's snow radar data to provide evidence for a gradual ablation of ~16-18 m of firn (~200 years of accumulation) from wind-scour zones over the upper Recovery Ice Stream catchment. The maximum ablation rates observed in this region are ~ -54 kg m-2 a-1 (-54 mm water equivalent a-1). Our airborne radio echo-sounding analysis show snow redeposition downslope of the wind-scour zones is <10% of the cumulative mass loss. Our study shows that the local mass loss is dominated by sublimation to water vapor rather than wind-transport of snow.

  5. Bounding the rate of moment deficit accumulation along the Tohoku segment using GEONET GPS data

    NASA Astrophysics Data System (ADS)

    Segall, P.; Johnson, K. M.; Miyazaki, S.

    2011-12-01

    Geodetic estimates of strain accumulation compared to the rate of past moment release provide important input to earthquake hazard forecasts. Prior to the March 2011 Tohoku earthquake a number of studies investigated plate coupling along the Japan Trench in NE Japan using GEONET GPS data. Most of these assumed an elastic back-slip framework, and regularized the underdetermined inverse problem by minimizing some norm of the back-slip rate. All studies apparently smoothed to zero coupling at the trench and therefore infer the highest coupling just offshore. In contrast, we estimate rigorous bounds on the maximum and minimum permissible rates of moment deficit (MDR) accumulation, not a ``favored'' model according to some ad hoc regularization, using methods of Johnson et al [1994] and Murray and Segall [2002]. Given a domain of interest (the Tohoku rupture segment) and Green's functions G relating slip to data, we solve the following optimization problem for back-slip rate m: {minimize}\\ || G m - d ||22 \\ {subject to} \\ A m = M0 \\ \\ {and} \\ \\ 0 ≤ mi ≤ vplate, where A = [1,1, ... 1], such that A m yields the (normalized) moment-rate. on the model domain. Preliminary results find that || G m - d ||22 exhibits a broad minimum over a factor of 2 in MDR. For the minimum MDR the locked zone is just offshore, while the plate-boundary near the trench slips at the plate rate. At the maximum MDR the locked zone is much larger, including the entire fault near the trench. This clearly demonstrates that the shallow fault is completely in the null-space for onshore data, and that there is at least a factor of two uncertainty in MDR. We will present results employing a bootstrap procedure that is independent of an assumed error distribution in the GPS data. We also extend the method to include viscoelastic earthquake cycle effects, including time-dependence due both to past earthquakes and steady backslip. In these models, fault locking near the trench induces flow which

  6. Faster evolving Drosophila paralogs lose expression rate and ubiquity and accumulate more non-synonymous SNPs

    PubMed Central

    2014-01-01

    Background Duplicated genes can indefinately persist in genomes if either both copies retain the original function due to dosage benefit (gene conservation), or one of the copies assumes a novel function (neofunctionalization), or both copies become required to perform the function previously accomplished by a single copy (subfunctionalization), or through a combination of these mechanisms. Different models of duplication retention imply different predictions about substitution rates in the coding portion of paralogs and about asymmetry of these rates. Results We analyse sequence evolution asymmetry in paralogs present in 12 Drosophila genomes using the nearest non-duplicated orthologous outgroup as a reference. Those paralogs present in D. melanogaster are analysed in conjunction with the asymmetry of expression rate and ubiquity and of segregating non-synonymous polymorphisms in the same paralogs. Paralogs accumulate substitutions, on average, faster than their nearest singleton orthologs. The distribution of paralogs’ substitution rate asymmetry is overdispersed relative to that of orthologous clades, containing disproportionally more unusually symmetric and unusually asymmetric clades. We show that paralogs are more asymmetric in: a) clades orthologous to highly constrained singleton genes; b) genes with high expression level; c) genes with ubiquitous expression and d) non-tandem duplications. We further demonstrate that, in each asymmetrically evolving pair of paralogs, the faster evolving member of the pair tends to have lower average expression rate, lower expression uniformity and higher frequency of non-synonymous SNPs than its slower evolving counterpart. Conclusions Our findings are consistent with the hypothesis that many duplications in Drosophila are retained despite stabilising selection being more relaxed in one of the paralogs than in the other, suggesting a widespread unfinished pseudogenization. This phenomenon is likely to make detection of

  7. A molecular assessment of phylogenetic relationships and lineage accumulation rates within the family Salamandridae (Amphibia, Caudata).

    PubMed

    Weisrock, David W; Papenfuss, Theodore J; Macey, J Robert; Litvinchuk, Spartak N; Polymeni, Rosa; Ugurtas, Ismail H; Zhao, Ermi; Jowkar, Houman; Larson, Allan

    2006-11-01

    We examine phylogenetic relationships among salamanders of the family Salamandridae using approximately 2700 bases of new mtDNA sequence data (the tRNALeu, ND1, tRNAIle, tRNAGln, tRNAMet, ND2, tRNATrp, tRNAAla, tRNAAsn, tRNACys, tRNATyr, and COI genes and the origin for light-strand replication) collected from 96 individuals representing 61 of the 66 recognized salamandrid species and outgroups. Phylogenetic analyses using maximum parsimony and Bayesian analysis are performed on the new data alone and combined with previously reported sequences from other parts of the mitochondrial genome. The basal phylogenetic split is a polytomy of lineages ancestral to (1) the Italian newt Salamandrina terdigitata, (2) a strongly supported clade comprising the "true" salamanders (genera Chioglossa, Mertensiella, Lyciasalamandra, and Salamandra), and (3) a strongly supported clade comprising all newts except S. terdigitata. Strongly supported clades within the true salamanders include monophyly of each genus and grouping Chioglossa and Mertensiella as the sister taxon to a clade comprising Lyciasalamandra and Salamandra. Among newts, genera Echinotriton, Pleurodeles, and Tylototriton form a strongly supported clade whose sister taxon comprises the genera Calotriton, Cynops, Euproctus, Neurergus, Notophthalmus, Pachytriton, Paramesotriton, Taricha, and Triturus. Our results strongly support monophyly of all polytypic newt genera except Paramesotriton and Triturus, which appear paraphyletic, and Calotriton, for which only one of the two species is sampled. Other well-supported clades within newts include (1) Asian genera Cynops, Pachytriton, and Paramesotriton, (2) North American genera Notophthalmus and Taricha, (3) the Triturus vulgaris species group, and (4) the Triturus cristatus species group; some additional groupings appear strong in Bayesian but not parsimony analyses. Rates of lineage accumulation through time are evaluated using this nearly comprehensive sampling of

  8. Amazonian chemical weathering rate derived from stony meteorite finds at Meridiani Planum on Mars

    NASA Astrophysics Data System (ADS)

    Schröder, Christian; Bland, Phil A.; Golombek, Matthew P.; Ashley, James W.; Warner, Nicholas H.; Grant, John A.

    2016-11-01

    Spacecraft exploring Mars such as the Mars Exploration Rovers Spirit and Opportunity, as well as the Mars Science Laboratory or Curiosity rover, have accumulated evidence for wet and habitable conditions on early Mars more than 3 billion years ago. Current conditions, by contrast, are cold, extremely arid and seemingly inhospitable. To evaluate exactly how dry today's environment is, it is important to understand the ongoing current weathering processes. Here we present chemical weathering rates determined for Mars. We use the oxidation of iron in stony meteorites investigated by the Mars Exploration Rover Opportunity at Meridiani Planum. Their maximum exposure age is constrained by the formation of Victoria crater and their minimum age by erosion of the meteorites. The chemical weathering rates thus derived are ~1 to 4 orders of magnitude slower than that of similar meteorites found in Antarctica where the slowest rates are observed on Earth.

  9. Amazonian chemical weathering rate derived from stony meteorite finds at Meridiani Planum on Mars

    PubMed Central

    Schröder, Christian; Bland, Phil A.; Golombek, Matthew P.; Ashley, James W.; Warner, Nicholas H.; Grant, John A.

    2016-01-01

    Spacecraft exploring Mars such as the Mars Exploration Rovers Spirit and Opportunity, as well as the Mars Science Laboratory or Curiosity rover, have accumulated evidence for wet and habitable conditions on early Mars more than 3 billion years ago. Current conditions, by contrast, are cold, extremely arid and seemingly inhospitable. To evaluate exactly how dry today's environment is, it is important to understand the ongoing current weathering processes. Here we present chemical weathering rates determined for Mars. We use the oxidation of iron in stony meteorites investigated by the Mars Exploration Rover Opportunity at Meridiani Planum. Their maximum exposure age is constrained by the formation of Victoria crater and their minimum age by erosion of the meteorites. The chemical weathering rates thus derived are ∼1 to 4 orders of magnitude slower than that of similar meteorites found in Antarctica where the slowest rates are observed on Earth. PMID:27834377

  10. Amazonian chemical weathering rate derived from stony meteorite finds at Meridiani Planum on Mars.

    PubMed

    Schröder, Christian; Bland, Phil A; Golombek, Matthew P; Ashley, James W; Warner, Nicholas H; Grant, John A

    2016-11-11

    Spacecraft exploring Mars such as the Mars Exploration Rovers Spirit and Opportunity, as well as the Mars Science Laboratory or Curiosity rover, have accumulated evidence for wet and habitable conditions on early Mars more than 3 billion years ago. Current conditions, by contrast, are cold, extremely arid and seemingly inhospitable. To evaluate exactly how dry today's environment is, it is important to understand the ongoing current weathering processes. Here we present chemical weathering rates determined for Mars. We use the oxidation of iron in stony meteorites investigated by the Mars Exploration Rover Opportunity at Meridiani Planum. Their maximum exposure age is constrained by the formation of Victoria crater and their minimum age by erosion of the meteorites. The chemical weathering rates thus derived are ∼1 to 4 orders of magnitude slower than that of similar meteorites found in Antarctica where the slowest rates are observed on Earth.

  11. Verification of International Space Station Component Leak Rates by Helium Accumulation Method

    NASA Technical Reports Server (NTRS)

    Underwood, Steve D.; Smith, Sherry L.

    2003-01-01

    Discovery of leakage on several International Space Station U.S. Laboratory Module ammonia system quick disconnects (QDs) led to the need for a process to quantify total leakage without removing the QDs from the system. An innovative solution was proposed allowing quantitative leak rate measurement at ambient external pressure without QD removal. The method utilizes a helium mass spectrometer configured in the detector probe mode to determine helium leak rates inside a containment hood installed on the test component. The method was validated through extensive developmental testing. Test results showed the method was viable, accurate and repeatable for a wide range of leak rates. The accumulation method has been accepted by NASA and is currently being used by Boeing Huntsville, Boeing Kennedy Space Center and Boeing Johnson Space Center to test welds and valves and will be used by Alenia to test the Cupola. The method has been used in place of more expensive vacuum chamber testing which requires removing the test component from the system.

  12. Engineering alfalfa to accumulate useful caffeic acid derivatives and characterization of hydroxycinnamoyl-CoA transferases from legumes

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Some forages crops, such as red clover, accumulate high levels of caffeic acid derivatives. Oxidation of these o-diphenols to quinones by endogenous polyphenol oxidases (PPOs) and the subsequent reactions of these quinones (probably with endogenous plant proteases) result in a significant reduction ...

  13. Equatorial Pacific Reactive Phosphorus Accumulation Rates across the Eocene/Oligocene Transition

    NASA Astrophysics Data System (ADS)

    Faul, K. L.; Stewart, K. L.

    2007-12-01

    Paired benthic foraminiferal stable isotope and trace metal records from ODP Site 1218 in the equatorial Pacific have shown that the Eocence/Oligocene (E/O) transition (~34 Ma) represents a major increase in Antarctic ice accumulation and a rapid deepening of the global calcite compensation depth (CCD) occurring in two steps [e.g., Lear et al., 2000; Lear et al., 2004; Coxall et al., 2005; Palike et al., 2006]. Simultaneous increases in biogenic mass accumulation rates (MARs) around Australia and in the Atlantic Ocean have been interpreted as representing increased productivity and/or organic carbon (C) burial possibly linked to cooling and ice sheet growth [Diester-Hass and Zahn, 2001; Anderson and Delaney, 2005]. Because the global CCD deepens across the transition, there is a need to distinguish organic C burial changes from CCD changes. We are determining reactive phosphorus (P, micromol P cm-2 kyr-1) MARs as an indicator of organic C burial for Site 1218 to help constrain the relative role of productivity during the E/O climatic transition. Reactive phosphorus (the sum of oxide associated, authigenic, and organic P; sequentially extracted from bulk sediment), delivered to the sediment water interface with organic C, is well preserved in oxygenated sediments. Preliminary results show an order of magnitude decrease in reactive P concentrations (from ~100 to ~10 micromol P g-1) across the E/O boundary. This may indicate that absolute organic C burial as well as the ratio of organic C burial to calcite burial may have decreased across the E/O transition in the equatorial Pacific upwelling zone.

  14. Tracing nitrogen accumulation in decaying wood and examining its impact on wood decomposition rate

    NASA Astrophysics Data System (ADS)

    Rinne, Katja T.; Rajala, Tiina; Peltoniemi, Krista; Chen, Janet; Smolander, Aino; Mäkipää, Raisa

    2016-04-01

    Decomposition of dead wood, which is controlled primarily by fungi is important for ecosystem carbon cycle and has potentially a significant role in nitrogen fixation via diazotrophs. Nitrogen content has been found to increase with advancing wood decay in several studies; however, the importance of this increase to decay rate and the sources of external nitrogen remain unclear. Improved knowledge of the temporal dynamics of wood decomposition rate and nitrogen accumulation in wood as well as the drivers of the two processes would be important for carbon and nitrogen models dealing with ecosystem responses to climate change. To tackle these questions we applied several analytical methods on Norway spruce logs from Lapinjärvi, Finland. We incubated wood samples (density classes from I to V, n=49) in different temperatures (from 8.5oC to 41oC, n=7). After a common seven day pre-incubation period at 14.5oC, the bottles were incubated six days in their designated temperature prior to CO2 flux measurements with GC to determine the decomposition rate. N2 fixation was measured with acetylene reduction assay after further 48 hour incubation. In addition, fungal DNA, (MiSeq Illumina) δ15N and N% composition of wood for samples incubated at 14.5oC were determined. Radiocarbon method was applied to obtain age distribution for the density classes. The asymbiotic N2 fixation rate was clearly dependent on the stage of wood decay and increased from stage I to stage IV but was substantially reduced in stage V. CO2 production was highest in the intermediate decay stage (classes II-IV). Both N2 fixation and CO2 production were highly temperature sensitive having optima in temperature 25oC and 31oC, respectively. We calculated the variation of annual levels of respiration and N2 fixation per hectare for the study site, and used the latter data together with the 14C results to determine the amount of N2 accumulated in wood in time. The proportion of total nitrogen in wood

  15. Amiodarone increases the accumulation of DEA in a human alveolar epithelium-derived cell line.

    PubMed

    Seki, Satoru; Itagaki, Shirou; Kobayashi, Masaki; Hirano, Takeshi; Iseki, Ken

    2008-07-01

    Amiodarone (AMD)-induced pulmonary toxicity (AIPT) is the most life-threatening side-effect of AMD treatment. N-Monodesethylamiodarone (DEA), an active metabolite of AMD, also exhibits cytotoxicity and tends to accumulate in the lung more intensively than AMD. In this study, we characterized the mechanism of DEA accumulation using A549 cells as a model of the alveolar epithelium. Typical ATP-depletion compounds caused an approximately 30% increase in the accumulation of DEA in A549 cells, although these effects were less than those in Caco-2 cells. Triiodothyronine (T(3)), which exhibited an inhibitory effect on DEA efflux in Caco-2 cells, did not affect the accumulation of DEA in A549 cells. On the other hand, 100 microM AMD caused an approximately 200% increase in DEA content in A549 cells, although AMD accumulation was not affected by 100 microM DEA. Since the reducing effect of AMD on cellular ATP levels and that of FCCP were similar, the mechanism by which DEA accumulation is increased by AMD might be different from the ATP-dependent DEA efflux mechanism. The decrease in cell viability by DEA in the presence of AMD (IC(50) value of DEA for A549 cell viability: 25.4+/-2.4 microM) was more pronounced than that by DEA alone (IC(50) value: 11.5+/-3.0 microM). This further DEA accumulation by AMD might be a factor responsible for the greater accumulation of DEA than that of AMD in the lung in long-term AMD-treated patients.

  16. Sediment accumulation rates in Conowingo Reservoir as determined by man-made and natural radionuclides

    SciTech Connect

    McLean, R.I.; Domotor, S.L. ); Summers, J.K.; Wilson, H. ); Olsen, C.R.; Larsen, I.L. )

    1991-05-01

    The Susquehanna River is the major contributor to sediment loadings in the Chesapeake Bay. Because many environmental contaminants are associated with suspended particulates, the degree of particle retention within the reservoirs of the lower Susquehanna River is an important consideration in evaluating contaminant loadings to the Chesapeake Bay. Profiles of weapons-test Cs-137, nuclear power plant-related Cs-134 and Cs-137, and naturally-derived Pb-210 were used to estimate rates of sediment accretion in the Conowingo Reservoir,an impoundmment of the Susquehanna River along the Maryland-Pennsylvania border. Net accretion rates ranged from about 2 cm yr{sup {minus}1} downstream of a nuclear power plant cooling discharge to a high of about 7 cm yr{sup {minus}1} at the mount of an incoming creek. Slight, but consistent, increases in the annual rate of accretion since the creation of the reservoir in 1928 are apparent. The current net average annual sediment load retained by the reservoir is estimated to be 0.4 {times} 10{sup 6} to 1.5 {times} 10{sup 6} metric tons yr{sup {minus}1}. The retained sediment load represents about 8-23% of the long-time average sediment input to the reservoir.

  17. Elucidating effects of atmospheric deposition and peat decomposition processes on mercury accumulation rates in a northern Minnesota peatland over last 10,000 cal years

    NASA Astrophysics Data System (ADS)

    Nater, E. A.; Furman, O.; Toner, B. M.; Sebestyen, S. D.; Tfaily, M. M.; Chanton, J.; Fissore, C.; McFarlane, K. J.; Hanson, P. J.; Iversen, C. M.; Kolka, R. K.

    2014-12-01

    Climate change has the potential to affect mercury (Hg), sulfur (S) and carbon (C) stores and cycling in northern peatland ecosystems (NPEs). SPRUCE (Spruce and Peatland Responses Under Climate and Environmental change) is an interdisciplinary study of the effects of elevated temperature and CO2 enrichment on NPEs. Peat cores (0-3.0 m) were collected from 16 large plots located on the S1 peatland (an ombrotrophic bog treed with Picea mariana and Larix laricina) in August, 2012 for baseline characterization before the experiment begins. Peat samples were analyzed at depth increments for total Hg, bulk density, humification indices, and elemental composition. Net Hg accumulation rates over the last 10,000 years were derived from Hg concentrations and peat accumulation rates based on peat depth chronology established using 14C and 13C dating of peat cores. Historic Hg deposition rates are being modeled from pre-industrial deposition rates in S1 scaled by regional lake sediment records. Effects of peatland processes and factors (hydrology, decomposition, redox chemistry, vegetative changes, microtopography) on the biogeochemistry of Hg, S, and other elements are being assessed by comparing observed elemental depth profiles with accumulation profiles predicted solely from atmospheric deposition. We are using principal component analyses and cluster analyses to elucidate relationships between humification indices, peat physical properties, and inorganic and organic geochemistry data to interpret the main processes controlling net Hg accumulation and elemental concentrations in surface and subsurface peat layers. These findings are critical to predicting how climate change will affect future accumulation of Hg as well as existing Hg stores in NPE, and for providing reference baselines for SPRUCE future investigations.

  18. Millennial Rates of Sea Cliff Retreat Derived From Cosmogenic 10Be and Coastal Platform Morphology

    NASA Astrophysics Data System (ADS)

    Hurst, M. D.; Ellis, M. A.; Rood, D. H.

    2014-12-01

    Observation of cliff erosion are often limited to relatively short timescales (a few decades), which are within the timeframe of anthropogenic modification of the coast and may be shorter than the recurrence interval for erosion events. Here we present long-term (centennial-millennial) averaged rates of sea cliff retreat for chalk cliffs in SE England derived from cosmogenic isotopes and coastal morphology. We determine long-term rates of sea cliff erosion from 10Be measured from in situ flint samples collected from three transects across the coastal platform in East Sussex. A numerical model of 10Be accumulation on an evolving coastal profile allows estimation of cliff retreat rate averaged over several hundred years. The model accounts for variation in 10Be accumulation with tides and sea-level rise, and takes into account platform downwear and topographic shielding by adjacent cliffs. Additionally, we use high-resolution (1m) multibeam bathymetry to map the extent of the coastal platform based on the surface texture in order to infer the position of the coast at ~8 ka. The difference in position to the current coastline provides estimates of Holocene-averaged rates of cliff erosion for all chalk cliffed coastline in the region. Comparison to historic records of cliff retreat reveals key similarities and differences between long and short-term signals. In certain locations, there are significant discrepancies (either faster or slower) between historic records and long-term rates of retreat. Each type of discrepancy may be the result of human interaction with the coastal environment, whether that interaction is local or non-local, and it is worthwhile noting that sites of relatively low historic rates of erosion are likely subject to high-magnitude, low-frequency failure events that could have devastating effects on human lives and infrastructure in areas that are considered to be low risk.

  19. Characteristics of the accumulation of methotrexate polyglutamate derivatives in Ehrlich ascites tumor cells and isolated rat hepatocytes

    SciTech Connect

    Fry, D.W.; Gewirtz, D.A.; Yalowich, J.C.; Goldman, I.D.

    1983-01-01

    The intracellular synthesis and retention of polygammaglutamyl derivatives of methotrexate and their interactions with H/sub 2/ folate reductase was evaluated. Methotrexate polyglutamates were detected within 15 minutes in hepatocytes exposed to 1 microM methotrexate, and continued to accumulate for at least 60 minutes producing a large transmembrane gradient. These derivatives appeared to be preferentially retained within the cell. In studies with the Ehrlich ascites tumor accumulation of methotrexate polyglutamates was increased over 5-fold by the addition of 5 mM L-glutamine or L-glutamate and exhibited a positive correlation with the extracellular concentration of methotrexate. When Ehrlich ascites tumor cells were exposed to 10 microM methotrexate and 5 mM L-glutamine intracellular polyglutamates were detected within 10 minutes and their levels increased linearly over 4 hours. As these derivatives accumulated, there was a decline in intracellular methotrexate due at least in part to a replacement of methotrexate on H/sub 2/ folate reductase by polyglutamates and subsequent efflux of the previously bound methotrexate from the cell. When polyglutamate derivatives were in excess of the H/sub 2/ folate reductase binding capacity and extracellular methotrexate removed, methotrexate rapidly exited the cell whereas the majority of its metabolites were retained and eventually saturated the major portion of the enzyme. These studies indicate that (1) intracellular methotrexate is rapidly converted to polygammaglutamyl derivatives, (2) these metabolites effectively compete with methotrexate for binding sites on H/sub 2/ folate reductase, (3) these derivatives are retained within the cell more effectively than methotrexate, and (4) vincristine and probenecid may be potentially useful for selectively increasing methotrexate polyglutamates in tumor cells.

  20. Accumulation rates and predominant atmospheric sources of natural and anthropogenic Hg and Pb on the Faroe Islands

    NASA Astrophysics Data System (ADS)

    Shotyk, W.; Goodsite, M. E.; Roos-Barraclough, F.; Givelet, N.; Le Roux, G.; Weiss, D.; Cheburkin, A. K.; Knudsen, K.; Heinemeier, J.; van Der Knaap, W. O.; Norton, S. A.; Lohse, C.

    2005-01-01

    A monolith representing 5420 14C yr of peat accumulation was collected from a blanket bog at Myrarnar, Faroe Islands. The maximum Hg concentration (498 ng/g at a depth of 4.5 cm) coincides with the maximum concentration of anthropogenic Pb (111 μg/g). Age dating of recent peat accumulation using 210Pb (CRS model) shows that the maxima in Hg and Pb concentrations occur at AD 1954 ± 2. These results, combined with the isotopic composition of Pb in that sample ( 206Pb/ 207Pb = 1.1720 ± 0.0017), suggest that coal burning was the dominant source of both elements. From the onset of peat accumulation (ca. 4286 BC) until AD 1385, the ratios Hg/Br and Hg/Se were constant (2.2 ± 0.5 × 10 -4 and 8.5 ± 1.8 × 10 -3, respectively). Since then, Hg/Br and Hg/Se values have increased, also reaching their maxima in AD 1954. The age date of the maximum concentrations of anthropogenic Hg and Pb in the Faroe Islands is consistent with a previous study of peat cores from Greenland and Denmark (dated using the atmospheric bomb pulse curve of 14C), which showed maximum concentrations in AD 1953. The average rate of atmospheric Hg accumulation from 1520 BC to AD 1385 was 1.27 ± 0.38 μg/m 2/yr. The Br and Se concentrations and the background Hg/Br and Hg/Se ratios were used to calculate the average rate of natural Hg accumulation for the same period, 1.32 ± 0.36 μg/m 2/yr and 1.34 ± 0.29 μg/m 2/yr, respectively. These fluxes are similar to the preanthropogenic rates obtained using peat cores from Switzerland, southern Greenland, southern Ontario, Canada, and the northeastern United States. Episodic volcanic emissions and the continual supply of marine aerosols to the Faroe Islands, therefore, have not contributed significantly to the Hg inventory or the Hg accumulation rates, relative to these other areas. The maximum rate of Hg accumulation was 34 μg/m 2/yr. The greatest fluxes of anthropogenic Hg accumulation calculated using Br and Se, respectively, were 26 and 31 μg/m 2

  1. River sediment flux and shelf sediment accumulation rates on the Pacific Northwest margin

    NASA Astrophysics Data System (ADS)

    Wheatcroft, R. A.; Sommerfield, C. K.

    2005-02-01

    To test the generality of insight obtained from recent STRATAFORM studies of northern California's Eel margin, river sediment sources and continental shelf sinks were examined on the Pacific Northwest margin from 38° to 44.5°N. River discharge and sediment concentration data acquired by the US Geological Survey were used to update long-term annual suspended-sediment loads for 17 rivers that range in basin area from 635 to ˜22,000 km 2. Resulting annual load estimates vary over 3 orders of magnitude (0.065-18×10 9 kg/yr), with major suspended-sediment fluxes supplied by, in decreasing order, the Eel, Klamath/Trinity, Mad, Rogue, Umpqua and Russian rivers. Down-core profiles of excess 210Pb and 137Cs were used to estimate sediment accumulation rates (SARs) at prescribed depths (70 and 110 m) and distances (0-40-km north and south along-shelf) from each of the major rivers. SARs were found to vary much less than the river flux estimates, and are mostly in the range of 1.5 to 6 mm/yr. Most significantly, shelf SARs on the other Pacific Northwest margins are only slightly less than those observed on the Eel shelf, implying that much higher proportions of riverine sediment are retained on those shelves. Likely reasons that the Eel dispersal system exhibits greater off-shelf transport are (1) the narrower and steeper shelf geometry, and (2) the existence of a newly documented cross-isobath sediment transport mechanism that involves wave-modulated fluid mud flows. Testing whether the fluid mud flows are a consequence of the Eel River basin's high sediment yield, and are thus unique to the Eel, or are caused by intense wave energy during discharge events, and hence are operative on many other margins, awaits future bottom-boundary layer measurements.

  2. 210Pb mass accumulation rates in the depositional area of the Magra River (Mediterranean Sea, Italy)

    NASA Astrophysics Data System (ADS)

    Delbono, I.; Barsanti, M.; Schirone, A.; Conte, F.; Delfanti, R.

    2016-08-01

    Nine sediment cores were collected between 2009 and 2012 in the inner continental shelf (Mediterranean Sea, Italy) mainly influenced by the Magra River, at water depths ranging from 11 to 64 m. Mass Accumulation Rates (MARs) were calculated through 210Pb analysed by Gamma spectrometry. Three different dating models (single and two-layer CF-CS, CRS) were applied to clay normalised 210Pbxs profiles and 137Cs was used to validate the 210Pb geochronology. The maximum MAR values (>2 g cm-2 yr-1) were found in the region adjacent to the Magra River mouth and outside the Gulf of La Spezia (0.9±0.1 g cm-2 yr-1 at St. 3-C6 and 4-C4). Results from 137Cs/210Pbxs ratios calculated in Surface Mixed Layers (SMLs) evidenced the coastal boundaries of the Magra River depositional area, which is very limited towards south. Differently, in the north-west sector, fine sediments are generally driven by the Ligurian Current and move towards north-west: at the deepest and most distant station from the River mouth, the MAR value is the lowest one in the study area. Few major Magra River floods occurred during the sediment core sampling period. By using the short-lived radioisotope 7Be as a tracer of river floods, a clear 7Be signature of 2009 flood is present at St. 1-SA1C. Finally, by analyzing the clay normalised 210Pbxs profiles, a decrease of its activity dating the years 1999 and 2000 is observed in four cores, corresponding to two major Magra River floods occurring in those years.

  3. Accumulate repeat accumulate codes

    NASA Technical Reports Server (NTRS)

    Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

    2004-01-01

    In this paper we propose an innovative channel coding scheme called 'Accumulate Repeat Accumulate codes' (ARA). This class of codes can be viewed as serial turbo-like codes, or as a subclass of Low Density Parity Check (LDPC) codes, thus belief propagation can be used for iterative decoding of ARA codes on a graph. The structure of encoder for this class can be viewed as precoded Repeat Accumulate (RA) code or as precoded Irregular Repeat Accumulate (IRA) code, where simply an accumulator is chosen as a precoder. Thus ARA codes have simple, and very fast encoder structure when they representing LDPC codes. Based on density evolution for LDPC codes through some examples for ARA codes, we show that for maximum variable node degree 5 a minimum bit SNR as low as 0.08 dB from channel capacity for rate 1/2 can be achieved as the block size goes to infinity. Thus based on fixed low maximum variable node degree, its threshold outperforms not only the RA and IRA codes but also the best known LDPC codes with the dame maximum node degree. Furthermore by puncturing the accumulators any desired high rate codes close to code rate 1 can be obtained with thresholds that stay close to the channel capacity thresholds uniformly. Iterative decoding simulation results are provided. The ARA codes also have projected graph or protograph representation that allows for high speed decoder implementation.

  4. Proposal for defining the relevance of drug accumulation derived from single dose study data for modified release dosage forms.

    PubMed

    Scheerans, Christian; Heinig, Roland; Mueck, Wolfgang

    2015-03-01

    Recently, the European Medicines Agency (EMA) published the new draft guideline on the pharmacokinetic and clinical evaluation of modified release (MR) formulations. The draft guideline contains the new requirement of performing multiple dose (MD) bioequivalence studies, in the case when the MR formulation is expected to show 'relevant' drug accumulation at steady state (SS). This new requirement reveals three fundamental issues, which are discussed in the current work: first, measurement for the extent of drug accumulation (MEDA) predicted from single dose (SD) study data; second, its relationship with the percentage residual area under the plasma concentration-time curve (AUC) outside the dosing interval (τ) after SD administration, %AUC(τ-∞)SD ; and third, the rationale for a threshold of %AUC(τ-∞)SD that predicts 'relevant' drug accumulation at SS. This work revealed that the accumulation ratio RA,AUC , derived from the ratio of the time-averaged plasma concentrations during τ at SS and after SD administration, respectively, is the 'preferred' MEDA for MR formulations. A causal relationship was derived between %AUC(τ-∞)SD and RA,AUC , which is valid for any drug (product) that shows (dose- and time-) linear pharmacokinetics regardless of the shape of the plasma concentration-time curve. Considering AUC thresholds from other guidelines together with the causal relationship between %AUC(τ-∞)SD and RA,AUC indicates that values of %AUC(τ-∞)SD ≤ 20%, resulting in RA,AUC ≤ 1.25, can be considered as leading to non-relevant drug accumulation. Hence, the authors suggest that 20% for %AUC(τ-∞)SD is a reasonable threshold and selection criterion between SD or MD study designs for bioequivalence studies of new MR formulations.

  5. Carbon accumulation rates recorded in the last 150years in tropical high mountain peatlands of the Atlantic Rainforest, SE - Brazil.

    PubMed

    Lourençato, Lúcio F; Caldeira, Pedro P; Bernardes, Marcelo C; Buch, Andressa C; Teixeira, Daniel C; Silva-Filho, Emmanoel V

    2017-02-01

    Peatlands are environmental matrices that store large amounts of organic carbon (TOC) and work as records of environmental changes. Recent record of organic carbon accumulated were assessed in two Forest National Parks, Itatiaia and Serra dos Órgãos in the Southeastern of Brazil. Based on organic and inorganic characterization, the cores from peatlands presented a predominance of organic material in an advanced stage of decomposition and those soils were classified as typical Haplosaprists Histosols. The combination of favorable topographic and climatic conditions led to rapid C accumulation across coastal mountain in the tropical peatlands studied, presenting an average accumulation rate of C, in the last century, of 194gCm(-2)yr(-1) about 7 higher times than the rate found in boreal and subarctic peatlands, those higher values may be related to changes in the hydrological cycle occurred since 1950s.

  6. Lipid-Derived Biofuels: Determination of Factors that Control Triglyceride Accumulation in Microalgae

    DTIC Science & Technology

    2012-10-30

    Accumulation in Microalgae Principal Investigator Dr. K.E. Cooksey Department of Microbiology 109 Lewis Hall Montana State University...cultures to find the extent to which previously published results can be considered general for microalgae . Task 2. Quantify growth kinetics and...diatoms cultures to find the extent to which previous results can be found to be general for microalgae . Before this project, detailed

  7. A quick algorithm of counting flow accumulation matrix for deriving drainage networks from a DEM

    NASA Astrophysics Data System (ADS)

    Wang, Yanping; Liu, Yonghe; Xie, Hongbo; Xiang, ZhongLin

    2011-06-01

    Computerized auto-extraction of drainage networks from Digital Elevation Model (DEM) has been widely used in hydrological modeling and relevant studies. Several essential procedures need to be implemented in eight-directional(D8) watershed delineation method, among which a problem need to be resolved is the lack of a high efficiency algorithm for quick and accurate computation of flow accumulation matrix involved in river network delineations. For the problem of depression filling, the algorithm presented by Oliver Planchon has resolved it. This study was aimed to develop a simple and quick algorithm for flow accumulation matrix computations. For this purpose, a simple and high efficiency algorithm of the time complexity of O(n) compared to the commonly used code of the time complexity of O(n2) orO(nlogn) , has been developed. Performance tests on this newly developed algorithm were conducted for different size of DEMs, and the results suggested that the algorithm has a linear time complexity with increasing sizes of DEM. The computation efficiency of this newly developed algorithm is many times higher than the commonly used code, and for a DEM of size 1000*1000, flow accumulation matrix computation can be completed within only several seconds compared with about few minutes needed by common used algorithms.

  8. In Vitro Increase in Chloroquine Accumulation Induced by Dihydroethano- and Ethenoanthracene Derivatives in Plasmodium falciparum-Parasitized Erythrocytes

    PubMed Central

    Pradines, Bruno; Alibert, Sandrine; Houdoin, Carole; Santelli-Rouvier, Christiane; Mosnier, Joel; Fusai, Thierry; Rogier, Christophe; Barbe, Jacques; Parzy, Daniel

    2002-01-01

    The effects of a series of dihydroethano- and ethenoanthracene derivatives on chloroquine (CQ) accumulation in CQ-susceptible strain 3D7 and CQ-resistant clone W2 were assessed. The levels of CQ accumulation increased little or none in CQ-susceptible strain 3D7 and generally increased markedly in CQ-resistant strain W2. At 10 μM, 28 compounds yielded cellular accumulation ratios (CARs) greater than that observed with CQ alone in W2. At 10 μM, in strain W2, 21 of 31 compounds had CQ CARs two or more times higher than that of CQ alone, 15 of 31 compounds had CQ CARs three or more times higher than that of CQ alone, 13 of 31 compounds had CQ CARs four or more times higher than that of CQ alone, and 9 of 31 compounds had CQ CARs five or more times higher than that of CQ alone. At 1 μM, 17 of 31 compounds had CQ CARs two or more times higher than that of CQ alone, 12 of 31 compounds had CQ CARs three or more times higher than that of CQ alone, 6 of 31 compounds had CQ CARs four or more times higher than that of CQ alone, and 3 of 31 compounds had CQ CARs five or more times higher than that of CQ alone. At 1 μM, 17 of 31 compounds were more potent inducers of CQ accumulation than verapamil and 12 of 31 compounds were more potent inducers of CQ accumulation than promethazine. The nature of the basic group seems to be associated with increases in the levels of CQ accumulation. At 1 and 10 μM, 10 of 14 and 13 of 14 compounds with amino group (amines and diamines), respectively, had CARs ≥3, while at 1 and 10 μM, only 1 of the 13 derivatives with amido groups had CARs ≥3. Among 12 of the 31 compounds which were more active inducers of CQ accumulation than promethazine at 1 μM, 10 had amino groups and 1 had an amido group. PMID:12069956

  9. Recent rates of carbon accumulation in montane fens ofYosemite National Park, California, U.S.A.

    USGS Publications Warehouse

    Drexler, Judith; Fuller, Christopher C.; Orlando, James L.; Moore, Peggy E.

    2016-01-01

    Little is known about recent rates of carbon storage in montane peatlands, particularly in the western United States. Here we report on recent rates of carbon accumulation (past 50 to 100 years) in montane groundwater-fed peatlands (fens) of Yosemite National Park in central California, U.S.A. Peat cores were collected at three sites ranging in elevation from 2070 to 2500 m. Core sections were analyzed for bulk density, % organic carbon, and 210Pb activities for dating purposes. Organic carbon densities ranged from 0.026 to 0.065 g C cm-3. Mean vertical accretion rates estimated using210Pb over the 50-year period from ∼1960 to 2011 and the 100-year period from ∼1910 to 2011 were 0.28 (standard deviation = ±0.09) and 0.18 (±-0.04) cm yr-1, respectively. Mean carbon accumulation rates over the 50- and 100-year periods were 95.4 (±25.4) and 74.7 (±17.2) g C m-2 yr-1, respectively. Such rates are similar to recent rates of carbon accumulation in rich fens in western Canada, but more studies are needed to definitively establish both the similarities and differences in peat formation between boreal and temperate montane fens.

  10. Laboratory Determination of Molybdenum Accumulation Rates as a Measure of Hypoxic Conditions

    EPA Science Inventory

    Redox sensitive metals, such as molybdenum (Mo), are enriched in reducing sediments due to authigenic fixation in anoxic interstitial waters of sediments. This study tested whether the process of fixation and accumulation of Mo in sediments could provide a geochemical indicator o...

  11. ACCUMULATION RATE OF MICROBIAL BIOMASS AT TWO PERMEABLE REACTIVE BARRIER SITES

    EPA Science Inventory

    Accumulation of mineral precipitates and microbial biomass are key factors that impact the long-term performance of in-situ Permeable Reactive Barriers for treating contaminated groundwater. Both processes can impact remedial performance by decreasing zero-valent iron reactivity...

  12. Highly anomalous accumulation rates of C and N recorded by a relic, free-floating peatland in Central Italy.

    PubMed

    Zaccone, Claudio; Lobianco, Daniela; Shotyk, William; Ciavatta, Claudio; Appleby, Peter G; Brugiapaglia, Elisabetta; Casella, Laura; Miano, Teodoro M; D'Orazio, Valeria

    2017-02-23

    Floating islands mysteriously moving around on lakes were described by several Latin authors almost two millennia ago. These fascinating ecosystems, known as free-floating mires, have been extensively investigated from ecological, hydrological and management points of view, but there have been no detailed studies of their rates of accumulation of organic matter (OM), organic carbon (OC) and total nitrogen (TN). We have collected a peat core 4 m long from the free-floating island of Posta Fibreno, a relic mire in Central Italy. This is the thickest accumulation of peat ever found in a free-floating mire, yet it has formed during the past seven centuries and represents the greatest accumulation rates, at both decadal and centennial timescale, of OM (0.63 vs. 0.37 kg/m(2)/yr), OC (0.28 vs. 0.18 kg/m(2)/yr) and TN (3.7 vs. 6.1 g/m(2)/yr) ever reported for coeval peatlands. The anomalously high accretion rates, obtained using (14)C age dating, were confirmed using (210)Pb and (137)Cs: these show that the top 2 m of Sphagnum-peat has accumulated in only ~100 years. As an environmental archive, Posta Fibreno offers a temporal resolution which is 10x greater than any terrestrial peat bog, and promises to provide new insight into environmental changes occurring during the Anthropocene.

  13. Highly anomalous accumulation rates of C and N recorded by a relic, free-floating peatland in Central Italy

    PubMed Central

    Zaccone, Claudio; Lobianco, Daniela; Shotyk, William; Ciavatta, Claudio; Appleby, Peter G.; Brugiapaglia, Elisabetta; Casella, Laura; Miano, Teodoro M.; D’Orazio, Valeria

    2017-01-01

    Floating islands mysteriously moving around on lakes were described by several Latin authors almost two millennia ago. These fascinating ecosystems, known as free-floating mires, have been extensively investigated from ecological, hydrological and management points of view, but there have been no detailed studies of their rates of accumulation of organic matter (OM), organic carbon (OC) and total nitrogen (TN). We have collected a peat core 4 m long from the free-floating island of Posta Fibreno, a relic mire in Central Italy. This is the thickest accumulation of peat ever found in a free-floating mire, yet it has formed during the past seven centuries and represents the greatest accumulation rates, at both decadal and centennial timescale, of OM (0.63 vs. 0.37 kg/m2/yr), OC (0.28 vs. 0.18 kg/m2/yr) and TN (3.7 vs. 6.1 g/m2/yr) ever reported for coeval peatlands. The anomalously high accretion rates, obtained using 14C age dating, were confirmed using 210Pb and 137Cs: these show that the top 2 m of Sphagnum-peat has accumulated in only ~100 years. As an environmental archive, Posta Fibreno offers a temporal resolution which is 10x greater than any terrestrial peat bog, and promises to provide new insight into environmental changes occurring during the Anthropocene. PMID:28230066

  14. [Estimating of decadal accumulation rates of heavy metals in surface rice soils in the Tai Lake region of China].

    PubMed

    Li, Lianqing; Pan, Genxing; Zhang, Pingjiu; Cheng, Jiemin; Zhu, Qiuhua; Qiu, Duosheng

    2002-05-01

    Estimation of decadal accumulation of some heavy metals in surface rice soils from the Tai Lake region, southern Jiangsu Province, China was made by means of calculating the monitoring data and/or analysis data of the archived soil samples. For the last decade, the estimated annual accumulation rate for Cu or Zn, Pb and Cd was 0.3-1 mg.(kg.a)-1, 0.2-1 mg.(kg.a)-1 and 0.3-3 micrograms.(kg.a)-1 respectively, the apparent pollution loading was, therefore, respectively 0.5-1 kg.(hm2.a)-1, 0.5-1.0 kg.(hm2.a)-1, 0.5-3.0 kg.(hm2.a)-1 [symbol: see text] 0.8-10 x 10(-3) kg.(hm2.a)-1. The accumulation rate for the content of available form was shown to be greater than that of total content. The non-point source pollution marked bigger contribution to the total annual loading for the Pb and Cd than the other source pollutions, while the Cd loading was prominently higher than those reported in Europe. These results may indicate that the food safety in this region may be constrained by the soil pollution of these heavy metals at high accumulation rates.

  15. Novel phosphatidylethanolamine derivatives accumulate in circulation in hyperlipidemic ApoE−/− mice and activate platelets via TLR2

    PubMed Central

    Biswas, Sudipta; Xin, Liang; Panigrahi, Soumya; Zimman, Alejandro; Wang, Hua; Yakubenko, Valentin P.; Byzova, Tatiana V.; Salomon, Robert G.

    2016-01-01

    A prothrombotic state and increased platelet reactivity are common in dyslipidemia and oxidative stress. Lipid peroxidation, a major consequence of oxidative stress, generates highly reactive products, including hydroxy-ω-oxoalkenoic acids that modify autologous proteins generating biologically active derivatives. Phosphatidylethanolamine, the second most abundant eukaryotic phospholipid, can also be modified by hydroxy-ω-oxoalkenoic acids. However, the conditions leading to accumulation of such derivatives in circulation and their biological activities remain poorly understood. We now show that carboxyalkylpyrrole-phosphatidylethanolamine derivatives (CAP-PEs) are present in the plasma of hyperlipidemic ApoE−/− mice. CAP-PEs directly bind to TLR2 and induces platelet integrin αIIbβ3 activation and P-selectin expression in a Toll-like receptor 2 (TLR2)-dependent manner. Platelet activation by CAP-PEs includes assembly of TLR2/TLR1 receptor complex, induction of downstream signaling via MyD88/TIRAP, phosphorylation of IRAK4, and subsequent activation of tumor necrosis factor receptor–associated factor 6. This in turn activates the Src family kinases, spleen tyrosine kinase and PLCγ2, and platelet integrins. Murine intravital thrombosis studies demonstrated that CAP-PEs accelerate thrombosis in TLR2-dependent manner and that TLR2 contributes to accelerate thrombosis in mice in the settings of hyperlipidemia. Our study identified the novel end-products of lipid peroxidation, accumulating in circulation in hyperlipidemia and inducing platelet activation by promoting cross-talk between innate immunity and integrin activation signaling pathways. PMID:27015965

  16. Carbon and sediment accumulation in the Everglades (USA) during the past 4000 years: rates, drivers, and sources of error

    USGS Publications Warehouse

    Glaser, Paul H.; Volin, John C.; Givnish, Thomas J.; Hansen, Barbara C. S.; Stricker, Craig A.

    2012-01-01

    Tropical and sub-tropical wetlands are considered to be globally important sources for greenhouse gases but their capacity to store carbon is presumably limited by warm soil temperatures and high rates of decomposition. Unfortunately, these assumptions can be difficult to test across long timescales because the chronology, cumulative mass, and completeness of a sedimentary profile are often difficult to establish. We therefore made a detailed analysis of a core from the principal drainage outlet of the Everglades of South Florida, to assess these problems and determine the factors that could govern carbon accumulation in this large sub-tropical wetland. Accelerator mass spectroscopy dating provided direct evidence for both hard-water and open-system sources of dating errors, whereas cumulative mass varied depending upon the type of method used. Radiocarbon dates of gastropod shells, nevertheless, seemed to provide a reliable chronology for this core once the hard-water error was quantified and subtracted. Long-term accumulation rates were then calculated to be 12.1 g m-2 yr-1 for carbon, which is less than half the average rate reported for northern and tropical peatlands. Moreover, accumulation rates remained slow and relatively steady for both organic and inorganic strata, and the slow rate of sediment accretion ( 0.2 mm yr-1) tracked the correspondingly slow rise in sea level (0.35 mm yr-1 ) reported for South Florida over the past 4000 years. These results suggest that sea level and the local geologic setting may impose long-term constraints on rates of sediment and carbon accumulation in the Everglades and other wetlands.

  17. T1 Relaxation Rate (R1) Indicates Nonlinear Mn Accumulation in Brain Tissue of Welders With Low-Level Exposure

    PubMed Central

    Lee, Eun-Young; Flynn, Michael R.; Du, Guangwei; Lewis, Mechelle M.; Fry, Rebecca; Herring, Amy H.; Van Buren, Eric; Van Buren, Scott; Smeester, Lisa; Kong, Lan; Yang, Qing; Mailman, Richard B.; Huang, Xuemei

    2015-01-01

    Although the essential element manganese (Mn) is neurotoxic at high doses, the effects of lower exposure are unclear. MRI T1-weighted (TIW) imaging has been used to estimate brain Mn exposure via the pallidal index (PI), defined as the T1W intensity ratio in the globus pallidus (GP) versus frontal white matter (FWM). PI may not, however, be sensitive to Mn in GP because Mn also may accumulate in FWM. This study explored: (1) whether T1 relaxation rate (R1) could quantify brain Mn accumulation more sensitively; and (2) the dose-response relationship between estimated Mn exposure and T1 relaxation rate (R1). Thirty-five active welders and 30 controls were studied. Occupational questionnaires were used to estimate hours welding in the past 90 days (HrsW) and lifetime measures of Mn exposure. T1W imaging and T1-measurement were utilized to generate PI and R1 values in brain regions of interest (ROIs). PI did not show a significant association with any measure of Mn and/or welding-related exposure. Conversely, in several ROIs, R1 showed a nonlinear relationship to HrsW, with R1 signal increasing only after a critical exposure was reached. The GP had the greatest rate of Mn accumulation. Welders with higher exposure showed significantly higher R1 compared either with controls or with welders with lower exposure. Our data are additional evidence that Mn accumulation can be assessed more sensitively by R1 than by PI. Moreover, the nonlinear relationship between welding exposure and Mn brain accumulation should be considered in future studies and policies. PMID:25953701

  18. Mutation accumulation in real branches: fitness assays for genomic deleterious mutation rate and effect in large-statured plants.

    PubMed

    Schultz, Stewart T; Scofield, Douglas G

    2009-08-01

    The genomic deleterious mutation rate and mean effect are central to the biology and evolution of all species. Large-statured plants, such as trees, are predicted to have high mutation rates due to mitotic mutation and the absence of a sheltered germ line, but their size and generation time has hindered genetic study. We develop and test approaches for estimating deleterious mutation rates and effects from viability comparisons within the canopy of large-statured plants. Our methods, inspired by E. J. Klekowski, are a modification of the classic Bateman-Mukai mutation-accumulation experiment. Within a canopy, cell lineages accumulate mitotic mutations independently. Gametes or zygotes produced at more distal points by these cell lineages contain more mitotic mutations than those at basal locations, and within-flower selfs contain more homozygous mutations than between-flower selfs. The resulting viability differences allow demonstration of lethal mutation with experiments similar in size to assays of genetic load and allow estimates of the rate and effect of new mutations with moderate precision and bias similar to that of classic mutation-accumulation experiments in small-statured organisms. These methods open up new possibilities with the potential to provide valuable new insights into the evolutionary genetics of plants.

  19. Accumulation of accident-derived radiocesium in lake and coastal sediments at 300-700 km distance from Fukushima area.

    PubMed

    Ochiai, S; Miyata, Y; Nagao, S; Yamamoto, M; Murakami, T; Nishimura, S; Itono, T; Suzuki, T; Hamataka, K; Kawano, Y; Hamajima, Y; Kashiwaya, K

    2015-11-01

    The accumulation of accident-derived radiocesium was investigated in nine water bodies located 300-700 km from the Fukushima Dai-ichi Nuclear Power Plant (FDNPP). (134)Cs from the accident was detected in surface sediment of five water bodies. The (134)Cs concentration, corrected to the time of the accident in 2011, was generally lower than that of (137)Cs, and its spatial pattern does not fully correspond to that of (137)Cs. These results suggest that radiocesium derived from both FDNPP and past global fallout can be separately observed and that the contributions of both sources are non-uniform within these sites. The (134)Cs inventory in surface sediments is smaller than its deposition, suggesting that almost all deposited (134)Cs remains within the catchment and/or a part has been discharged from the saline and brackish water bodies.

  20. Cerebral accumulation of dietary derivable plant sterols does not interfere with memory and anxiety related behavior in Abcg5-/- mice.

    PubMed

    Vanmierlo, Tim; Rutten, Kris; van Vark-van der Zee, Leonie C; Friedrichs, Silvia; Bloks, Vincent W; Blokland, Arjan; Ramaekers, Frans C; Sijbrands, Eric; Steinbusch, Harry; Prickaerts, Jos; Kuipers, Folkert; Lütjohann, Dieter; Mulder, Monique

    2011-06-01

    Plant sterols such as sitosterol and campesterol are frequently applied as functional food in the prevention of atherosclerosis. Recently, it became clear that plasma derived plant sterols accumulate in murine brains. We questioned whether plant sterols in the brain are associated with alterations in brain cholesterol homeostasis and subsequently with brain functions. ATP binding cassette (Abc)g5-/- mice, a phytosterolemia model, were compared to Abcg5+/+ mice for serum and brain plant sterol accumulation and behavioral and cognitive performance. Serum and brain plant sterol concentrations were respectively 35-70-fold and 5-12-fold increased in Abcg5-/- mice (P<0.001). Plant sterol accumulation resulted in decreased levels of desmosterol (P<0.01) and 24(S)-hydroxycholesterol (P<0.01) in the hippocampus, the brain region important for learning and memory functions, and increased lanosterol levels (P<0.01) in the cortex. However, Abcg5-/- and Abcg5+/+ displayed no differences in memory functions or in anxiety and mood related behavior. The swimming speed of the Abcg5-/- mice was slightly higher compared to Abcg5+/+ mice (P<0.001). In conclusion, plant sterols in the brains of Abcg5-/- mice did have consequences for brain cholesterol metabolism, but did not lead to an overt phenotype of memory or anxiety related behavior. Thus, our data provide no contra-indication for nutritional intake of plant sterol enriched nutrition.

  1. Rates of Species Accumulation and Taxonomic Diversification during Phototrophic Biofilm Development Are Controlled by both Nutrient Supply and Current Velocity

    PubMed Central

    2013-01-01

    The accumulation of new and taxonomically diverse species is a marked feature of community development, but the role of the environment in this process is not well understood. To address this problem, we subjected periphyton in laboratory streams to low (10-cm · s−1), high (30-cm · s−1), and variable (9- to 32-cm · s−1) current velocity and low- versus high-nutrient inputs. We examined how current velocity and resource supply constrained (i) the rates of species accumulation, a measure of temporal beta-diversity, and (ii) the rates of diversification of higher taxonomic categories, defined here as the rate of higher taxon richness increase with the increase of species richness. Temporal biofilm dynamics were controlled by a strong nutrient-current interaction. Nutrients accelerated the rates of accumulation of new species, when flow velocity was not too stressful. Species were more taxonomically diverse under variable than under low-flow conditions, indicating that flow heterogeneity increased the niche diversity in the high-nutrient treatments. Conversely, the lower diversification rates under high- than under low-nutrient conditions at low velocity are explained with finer resource partitioning among species, belonging to a limited number of related genera. The overall low rates of diversification in high-current treatments suggest that the ability to withstand current stress was conserved within closely related species. Temporal heterogeneity of disturbance has been shown to promote species richness, but here we further demonstrate that it also affects two other components of biodiversity, i.e., temporal beta-diversity and diversification rate. Therefore, management efforts for preserving the inherent temporal heterogeneity of natural ecosystems will have detectable positive effects on biodiversity. PMID:23335757

  2. Using Derivatives to Hedge Interest Rate Risk: A Student Exercise

    ERIC Educational Resources Information Center

    Donaldson, Jeff; Flagg, Donald

    2014-01-01

    In a world of fluctuating asset prices, many firms find the need to hedge in order to avoid or reduce losses. From a gold miner selling gold derivatives to airlines buying oil futures to protect against rising fuel costs, hedging is common practice across many different industries. In this paper, we provide students with a simplified example of a…

  3. Nonpeptidic Lysosomal Modulators Derived from Z-Phe-Ala-Diazomethylketone for Treating Protein Accumulation Diseases

    PubMed Central

    2012-01-01

    Lysosomes are involved in protein turnover and removing misfolded species, and their enzymes have the potential to offset the defect in proteolytic clearance that contributes to the age-related dementia Alzheimer's disease (AD). The weak cathepsin B and L inhibitor Z-Phe-Ala-diazomethylketone (PADK) enhances lysosomal cathepsin levels at low concentrations, thereby eliciting protective clearance of PHF-τ and Aβ42 in the hippocampus and other brain regions. Here, a class of positive modulators is established with compounds decoupled from the cathepsin inhibitory properties. We utilized PADK as a departure point to develop nonpeptidic structures with the hydroxyethyl isostere. The first-in-class modulators SD1002 and SD1003 exhibit improved levels of cathepsin up-regulation but almost complete removal of cathepsin inhibitory properties as compared to PADK. Isomers of the lead compound SD1002 were synthesized, and the modulatory activity was determined to be stereoselective. In addition, the lead compound was tested in transgenic mice with results indicating protection against AD-type protein accumulation pathology. PMID:24900408

  4. Reduced inflammation in the tumor microenvironment delays the accumulation of myeloid-derived suppressor cells and limits tumor progression.

    PubMed

    Bunt, Stephanie K; Yang, Linglin; Sinha, Pratima; Clements, Virginia K; Leips, Jeff; Ostrand-Rosenberg, Suzanne

    2007-10-15

    Chronic inflammation is frequently associated with malignant growth and is thought to promote and enhance tumor progression, although the mechanisms which regulate this relationship remain elusive. We reported previously that interleukin (IL)-1beta promoted tumor progression by enhancing the accumulation of myeloid-derived suppressor cells (MDSC), and hypothesized that inflammation leads to cancer through the production of MDSC which inhibit tumor immunity. If inflammation-induced MDSC promote tumor progression by blocking antitumor immunity, then a reduction in inflammation should reduce MDSC levels and delay tumor progression, whereas an increase in inflammation should increase MDSC levels and hasten tumor progression. We have tested this hypothesis using the 4T1 mammary carcinoma and IL-1 receptor (IL-1R)-deficient mice which have a reduced potential for inflammation, and IL-1R antagonist-deficient mice, which have an increased potential for inflammation. Consistent with our hypothesis, IL-1R-deficient mice have a delayed accumulation of MDSC and reduced primary and metastatic tumor progression. Accumulation of MDSC and tumor progression are partially restored by IL-6, indicating that IL-6 is a downstream mediator of the IL-1beta-induced expansion of MDSC. In contrast, excessive inflammation in IL-1R antagonist-deficient mice promotes the accumulation of MDSC and produces MDSC with enhanced suppressive activity. These results show that immune suppression by MDSC and tumor growth are regulated by the inflammatory milieu and support the hypothesis that the induction of suppressor cells which down-regulate tumor immunity is one of the mechanisms linking inflammation and cancer.

  5. Accumulation of plastic-derived chemicals in tissues of seabirds ingesting marine plastics.

    PubMed

    Tanaka, Kosuke; Takada, Hideshige; Yamashita, Rei; Mizukawa, Kaoruko; Fukuwaka, Masa-aki; Watanuki, Yutaka

    2013-04-15

    We analyzed polybrominated diphenyl ethers (PBDEs) in abdominal adipose of oceanic seabirds (short-tailed shearwaters, Puffinus tenuirostris) collected in northern North Pacific Ocean. In 3 of 12 birds, we detected higher-brominated congeners (viz., BDE209 and BDE183), which are not present in the natural prey (pelagic fish) of the birds. The same compounds were present in plastic found in the stomachs of the 3 birds. These data suggested the transfer of plastic-derived chemicals from ingested plastics to the tissues of marine-based organisms.

  6. Tapetosomes in Brassica Tapetum Accumulate Endoplasmic Reticulum–Derived Flavonoids and Alkanes for Delivery to the Pollen Surface[W

    PubMed Central

    Hsieh, Kai; Huang, Anthony H.C.

    2007-01-01

    Tapetosomes are abundant organelles in tapetum cells during the active stage of pollen maturation in Brassicaceae species. They possess endoplasmic reticulum (ER)–derived vesicles and oleosin-coated lipid droplets, but their overall composition and function have not been established. In situ localization analyses of developing Brassica napus anthers revealed flavonoids present exclusively in tapetum cells, first in an ER network along with flavonoid-3′-hydroxylase and then in ER-derived tapetosomes. Flavonoids were absent in the cytosol, elaioplasts, vacuoles, and nuclei. Subcellular fractionation of developing anthers localized both flavonoids and alkanes in tapetosomes. Subtapetosome fractionation localized flavonoids in ER-derived vesicles, and alkanes and oleosins in lipid droplets. After tapetum cell death, flavonoids, alkanes, and oleosins were located on mature pollen. In the Arabidopsis thaliana mutants tt12 and tt19 devoid of a flavonoid transporter, flavonoids were present in the cytosol in reduced amounts but absent in tapetosomes and were subsequently located on mature pollen. tt4, tt12, and tt19 pollen was more susceptible than wild-type pollen to UV-B irradiation on subsequent germination. Thus, tapetosomes accumulate ER-derived flavonoids, alkanes, and oleosins for discharge to the pollen surface upon cell death. This tapetosome-originated pollen coat protects the haploidic pollen from UV light damage and water loss and aids water uptake. PMID:17307923

  7. Tapetosomes in Brassica tapetum accumulate endoplasmic reticulum-derived flavonoids and alkanes for delivery to the pollen surface.

    PubMed

    Hsieh, Kai; Huang, Anthony H C

    2007-02-01

    Tapetosomes are abundant organelles in tapetum cells during the active stage of pollen maturation in Brassicaceae species. They possess endoplasmic reticulum (ER)-derived vesicles and oleosin-coated lipid droplets, but their overall composition and function have not been established. In situ localization analyses of developing Brassica napus anthers revealed flavonoids present exclusively in tapetum cells, first in an ER network along with flavonoid-3'-hydroxylase and then in ER-derived tapetosomes. Flavonoids were absent in the cytosol, elaioplasts, vacuoles, and nuclei. Subcellular fractionation of developing anthers localized both flavonoids and alkanes in tapetosomes. Subtapetosome fractionation localized flavonoids in ER-derived vesicles, and alkanes and oleosins in lipid droplets. After tapetum cell death, flavonoids, alkanes, and oleosins were located on mature pollen. In the Arabidopsis thaliana mutants tt12 and tt19 devoid of a flavonoid transporter, flavonoids were present in the cytosol in reduced amounts but absent in tapetosomes and were subsequently located on mature pollen. tt4, tt12, and tt19 pollen was more susceptible than wild-type pollen to UV-B irradiation on subsequent germination. Thus, tapetosomes accumulate ER-derived flavonoids, alkanes, and oleosins for discharge to the pollen surface upon cell death. This tapetosome-originated pollen coat protects the haploidic pollen from UV light damage and water loss and aids water uptake.

  8. On the Effect of Ramp Rate in Damage Accumulation of the CPV Die-Attach: Preprint

    SciTech Connect

    Bosco, N. S.; Silverman, T. J.; Kurtz, S. R.

    2012-06-01

    It is commonly understood that thermal cycling at high temperature ramp rates may activate unrepresentative failure mechanisms. Increasing the temperature ramp rate of thermal cycling, however, could dramatically reduce the test time required to achieve an equivalent amount of thermal fatigue damage, thereby reducing overall test time. Therefore, the effect of temperature ramp rate on physical damage in the CPV die-attach is investigated. Finite Element Model (FEM) simulations of thermal fatigue and thermal cycling experiments are made to determine if the amount of damage calculated results in a corresponding amount of physical damage measured to the die-attach for a variety of fast temperature ramp rates. Preliminary experimental results are in good agreement with simulations and reinforce the potential of increasing temperature ramp rates. Characterization of the microstructure and resulting fatigue crack in the die-attach suggest a similar failure mechanism across all ramp rates tested.

  9. Biomonitoring of genotoxic effects and elemental accumulation derived from air pollution in community urban gardens.

    PubMed

    Amato-Lourenco, Luís Fernando; Lobo, Debora Jã A; Guimarães, Eliane T; Moreira, Tiana Carla Lopes; Carvalho-Oliveira, Regiani; Saiki, Mitiko; Saldiva, Paulo Hilário Nascimento; Mauad, Thais

    2017-01-01

    Urban gardening is a growing global phenomenon with a positive impact on society. Despite several associated benefits, growing vegetables in urban gardens that are localized in highly polluted areas poses questions about the safety of the produced food. Therefore, the identification of risk factors that result in possible deleterious effects to human health is important for realizing all of the benefits to society. We evaluated the use of two biomonitoring methods in ten urban gardens of Sao Paulo city and one control site: the micronuclei frequencies for early tetrads of Tradescantia pallida (Rose) Hunt. cv. "Purpurea" Boom (hereafter, Trad-MCN) as a short-term indicator of genotoxic response and tree barks to quantify the accumulation of traffic-related chemical elements as a long-term biomarker of air pollution in urban gardens. Mature plants of Tradescantia pallida were exposed in each garden, and their inflorescences were sampled over three months. A random set of 300 early tetrads in 13 to 21 slides per garden were evaluated for micronuclei frequencies. Elemental concentrations in 428 tree barks samples from 107 different trees in the areas surrounding urban gardens were quantified using an energy dispersive X-ray fluorescence spectrometer. The frequency of Trad-MCN has a significant correlation with traffic variables and chemical elements related to road dust and tailpipe emissions deposited in tree barks. Negative associations between Trad-MCN and both the distance through traffic and the presence of vertical obstacles were observed in the community gardens. The Mn/Zn concentrations in tree barks were associated with increased Trad-MCN.

  10. Impact of Megadunes and Glaze Areas on Estimates of East Antarctic Mass Balance and Accumulation Rate Change

    NASA Astrophysics Data System (ADS)

    Scambos, T. A.; Fahnestock, M. A.; Shuman, C.; Haran, T. M.

    2006-12-01

    for megadunes, glaze regions, and undulations in East Antarctica (and elsewhere) may also impact estimates of changes in accumulation rate (changes in surface elevation due to accumulation increase or decrease) when those changes are made by satellite radar altimeters (e.g. Davis et al, 2005). Previous studies have demonstrated that the broad footprint of satellite radar altimeters interacts preferentially with local highs in the topography; however, local highs show an amplified accumulation signal relative to the regional net surface flux. Therefore, changes in the net surface flux would also be amplified at the local crests, and could cause satellite radar change detection values to be overestimated. We present a simple model of this effect based on the megadune field measurements.

  11. Radiocarbon-derived sedimentation rates in the Gulf of Mexico

    NASA Astrophysics Data System (ADS)

    Santschi, Peter H.; Rowe, Gilbert T.

    2008-12-01

    Sedimentation rates were determined for the northern Gulf of Mexico margin sediments at water depths ranging from 770 to 3560 m, using radiocarbon determinations of organic matter. Resulting sedimentation rates ranged from 3 to 15 cm/kyr, decreasing with increasing water depth. These rates agree with long-term sedimentation rates estimated previously using stratigraphic methods, and with estimates of sediment delivery rates by the Mississippi River to the northern Gulf of Mexico, but are generally higher by 1-2 orders of magnitude than those estimated by 210Pb xs methods. Near-surface slope sediments from 2737 m water depth in the Mississippi River fan were much older than the rest. They had minimum 14C ages of 16-27 kyr and δ13C values ranging from -24‰ to -26.5‰, indicating a terrestrial origin of organic matter. The sediments from this site were thus likely deposited by episodic mass wasting of slope sediment through the canyon, delineating the previously suggested main pathway of sediment and clay movement to abyssal Gulf sediments.

  12. The impact of EDTA on the rate of accumulation and root/shoot partitioning of cadmium in mature dwarf sunflowers.

    PubMed

    Meighan, Michelle M; Fenus, Taressa; Karey, Emma; MacNeil, Joseph

    2011-06-01

    In addition to increasing the mobility of metal ions in the soil solution, chelating agents such as EDTA have been reported to alter both the total metal accumulated by plants and its distribution within the plant structures. Here, mature Mini-Sun Hybrid dwarf sunflowers exposed to 300 μM Cd(2+) in hydroponic solution had initial translocation rates of at least 0.12 mmol kg(-1)h(-1) and reached leaf saturation levels within a day when a 3-fold molar excess of EDTA was used. EDTA also promoted cadmium transfer from roots to the shoots. A threefold excess of EDTA increased the translocation factor (TF) 100-fold, resulting in cadmium levels in the leaves of 580 μg g(-1) and extracting 1400 μg plant(-1). When plants were exposed to dissolved cadmium without EDTA, the vast majority of the metal remained bound to the exterior of the root. The initial accumulation could be successfully modeled with a standard biosorption pseudo second-order kinetic equation. Initial accumulation rates ranged from 0.0359 to 0.262 mg g(-1)min(-1). The cadmium binding could be cycled, and did not show evidence of saturation under the experimental conditions employed, suggesting it might be a viable biosorbant for aqueous cadmium.

  13. Accumulate-Repeat-Accumulate-Accumulate-Codes

    NASA Technical Reports Server (NTRS)

    Divsalar, Dariush; Dolinar, Sam; Thorpe, Jeremy

    2004-01-01

    Inspired by recently proposed Accumulate-Repeat-Accumulate (ARA) codes [15], in this paper we propose a channel coding scheme called Accumulate-Repeat-Accumulate-Accumulate (ARAA) codes. These codes can be seen as serial turbo-like codes or as a subclass of Low Density Parity Check (LDPC) codes, and they have a projected graph or protograph representation; this allows for a high-speed iterative decoder implementation using belief propagation. An ARAA code can be viewed as a precoded Repeat-and-Accumulate (RA) code with puncturing in concatenation with another accumulator, where simply an accumulator is chosen as the precoder; thus ARAA codes have a very fast encoder structure. Using density evolution on their associated protographs, we find examples of rate-lJ2 ARAA codes with maximum variable node degree 4 for which a minimum bit-SNR as low as 0.21 dB from the channel capacity limit can be achieved as the block size goes to infinity. Such a low threshold cannot be achieved by RA or Irregular RA (IRA) or unstructured irregular LDPC codes with the same constraint on the maximum variable node degree. Furthermore by puncturing the accumulators we can construct families of higher rate ARAA codes with thresholds that stay close to their respective channel capacity thresholds uniformly. Iterative decoding simulation results show comparable performance with the best-known LDPC codes but with very low error floor even at moderate block sizes.

  14. Early phase tumor accumulation of macromolecules: a great difference in clearance rate between tumor and normal tissues.

    PubMed

    Noguchi, Y; Wu, J; Duncan, R; Strohalm, J; Ulbrich, K; Akaike, T; Maeda, H

    1998-03-01

    The objective of this study was to investigate the molecular weight (MW) and time-dependence of the phenomenon termed "the enhanced permeability and retention" (EPR) effect in solid tumor, in particular to determine and define the early phase accumulation of macromolecules in tumor and normal tissues and the relationship between blood concentration and tissue clearance. As a model, radioiodinated N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers of MW ranging from 4.5 K to 800 K were administered i.v. to mice bearing sarcoma 180 tumor. Within 10 min all HPMA copolymers accumulated effectively in the tumor regardless of MW (1.0-1.5% of injected dose per g of tumor). However, higher MW copolymers (> 50 K) showed significantly increased tumor accumulation after 6 h, while the lower MW copolymers (< 40 K) were cleared rapidly from tumor tissue due to rapid diffusion back into the bloodstream. Blood clearance was also MW-dependent; the lower MW copolymers displayed rapid clearance, with kidney radioactivity of the copolymers of MW < 20 K representing 24% of injected dose per g kidney at 1 min after i.v. administration. Within 10 min these copolymers passed through the kidney and were excreted in the urine. Higher MW copolymers consistently showed kidney levels of 3-5% dose per g kidney in the early phase with no time-dependent accumulation in kidney. There was also no progressive accumulation in muscle or liver, regardless of polymer MW. These results suggest the "EPR effect" in solid tumor primarily arises from in the difference in clearance rate between the solid tumor and the normal tissues after initial penetration of the polymers into these tissues.

  15. A method to reconstruct past accumulation rates in alpine firn regions: A study on Fiescherhorn, Swiss Alps

    NASA Astrophysics Data System (ADS)

    Schwerzmann, Aurel; Funk, Martin; Blatter, Heinz; Lüthi, Martin; Schwikowski, Margit; Palmer, Anne

    2006-03-01

    Annual snow layers in the accumulation zone of glaciers become thinner by viscous deformation while moving to greater depth. The reconstruction of the accumulation rate history requires the correction of the thickness of annual layers. We apply a novel method to determine the vertical velocity by repeated survey with a caliper probe of grooves scratched into the wall of a borehole. With this information and the assumptions of steady state velocity and density fields, the correction of layer thicknesses can be determined without knowledge of the ice density profile and thus can be applied to boreholes without ice cores. The method is applied to a borehole on Fiescherhorn, Swiss Alps, resulting in a substantially larger correction than with the simpler method of Nye (1963).

  16. Spatial and temporal variability of snow accumulation rate at East Antarctic ice sheet in 1993-2010

    NASA Astrophysics Data System (ADS)

    Motoyama, H.; Suzuki, K.; Yamanouchi, T.; Kawamura, K.

    2012-04-01

    Snow stakes along the traverse routes have been observed for long term monitoring program 'the variation of ice sheet surface mass balance' from the 1960's by the Japanese Antarctic Research Expedition in Shirase glacier drainage basin, East Antarctica. During the traverse route between coastal S16 point (69 02'S, 40 03'E, 580m a.s.l.) to inland Dome Fuji (77 22'S, 39 42'E, 3,810m a.s.l.), the snow stake observations every 2 km have been carried out from 1993. Latest stake heights were measured in January 2011 and February 2011. Yearly net snow accumulations from S16 to Dome Fuji were calculated. Heavy snow events were shown in 1998, 2004, 2005, 2008-2009 and 2010. Otherwise, in 1994, 1996, 1999, 2000, 2001, 2002 and 2006, light snow events were observed. They were different in way accumulating spatial pattern depending on places. The yearly accumulation rates were compared with seasonal change of AAO-index (SAM). As a result, yearly accumulation rate and AAO-index showed the positive correlation. We would indicate the spatial distributions of air parcel origins. So we calculate air transport by using the NITRAM trajectory model (Tomikawa and Sato, 2005) and ERA-Interim meteorological data set in 1990-2009. The time duration is 5 days and we suppose the origin of air parcel is the point of trajectory at 5 days ago. The starting points are distributed on 1 deg. x 1 deg. grids over Antarctica and its altitude is 1,300m above the surface. We indicate the spatial distributions of air parcel origins to Antarctica. If there were high ratios of sea origin atmosphere in the inland, there was much snow. It is indicated that the humid air from the sea is the main origin of snowfall. But such relations were not seen on the coast. We try to understand the cause of heavy snow and light snow event.

  17. Measuring water accumulation rates using GRACE data in areas experiencing glacial isostatic adjustment: The Nelson River basin

    NASA Astrophysics Data System (ADS)

    Lambert, A.; Huang, J.; Kamp, G.; Henton, J.; Mazzotti, S.; James, T. S.; Courtier, N.; Barr, A. G.

    2013-12-01

    Recovery and Climate Experiment (GRACE) satellite-derived total water storage can be obscured by glacial isostatic adjustment. In order to solve this problem for the Nelson River drainage basin in Canada, a gravity rate map from 110 months (June 2002 to October 2011) of GRACE gravity data was corrected for glacial isostatic adjustment using an independent gravity rate map derived from updated GPS vertical velocities. The GPS-based map was converted to equivalent gravity rate using a transfer function developed from GPS and absolute-g data at colocated sites. The corrected GRACE gravity rate map revealed a major positive anomaly within the drainage basin, which was independently shown by hydrological data to be due to changes in water storage. The anomaly represents a cumulative increase at its center of about 340 mm of water, reflecting a progression from extreme drought to extremely wet conditions.

  18. Mercury accumulation in caged Corbicula: rate of uptake and seasonal variation.

    PubMed

    Neufeld, Douglas S G

    2010-09-01

    The uptake and seasonal fluctuations of total mercury were followed in caged and uncaged Asiatic clams, Corbicula fluminea, over a 1-year period in South River, Virginia. Mercury was rapidly accumulated in clams transplanted from a nominally uncontaminated site into cages on the contaminated South River, reaching 0.99 microg g(-1) dry mass within the first month. Resident clams moved to cages had higher mercury contents after the first month (2.04 microg g(-1) dry mass) and at all subsequent times in the study. Large monthly fluctuations in mercury were noted for both resident caged and transplant caged clams with a notable peak occurring in early spring (4.31 microg g(-1) dry mass in resident caged clams). Tissue mass of caged clams steadily increased through the winter and early spring. Adjustment of mercury concentrations for tissue mass changes indicated that the changes in mercury contents were primarily due to uptake/release rather than changes in tissue mass (concentration/dilution). The present study demonstrates the utility of using caged Corbicula as mercury biomonitors and illustrates the importance of accounting for large, short-term changes of mercury content in Corbicula when designing long-term biomonitoring studies.

  19. Observations of historical sea cliff retreat rates exceed long-term estimates derived from cosmogenic 10Be

    NASA Astrophysics Data System (ADS)

    Hurst, Martin D.; Rood, Dylan H.; Ellis, Michael A.; Anderson, Robert S.

    2015-04-01

    Historical observation of coastal retreat are limited to relatively short timescales (< 150 years), during which time humans may have modified the coastal environment. There is growing concern that rates of coastal change may be accelerated in the face of anticipated stormier climates and rising sea level, yet there is little knowledge of rates of coastal change prior to the relatively brief historical records. In order to make predictions about potential future coastal change it is important to establish baseline conditions averaged over longer time periods. Here we present analysis of sea cliff retreat throughout the Holocene averaged for chalk cliffs in south-east England using cosmogenic isotopes. We determine long-term rates of sea cliff erosion from 10Be measured from in-situ flint samples collected from three transects across coastal platforms in East Sussex. A numerical model of 10Be accumulation on an evolving coastal profile allows estimation of cliff retreat rate during the Holocene. The model accounts for variation in 10Be accumulation with tides and sea-level rise, and takes into account platform downwear and topographic shielding by adjacent cliffs. We find that cliff retreat rates during the Holocene were significantly slower (2-6 cm yr-1) than those derived from recent historical observations (15-25 cm yr-1). Modelled accumulation of 10Be requires retreat rates that increase rapidly in recent times, potentially reflecting human modification of the coastal sediment budget through construction of sea defences, flood defenses and aggregate extraction. Therefore knowledge of past human activity at the coastline may be important in anticipating future rates of coastal retreat.

  20. Influence of conversion of penicillin G into a basic derivative on its accumulation and subcellular localization in cultured macrophages

    SciTech Connect

    Renard, C.; Vanderhaeghe, H.J.; Claes, P.J.; Zenebergh, A.; Tulkens, P.M.

    1987-03-01

    beta-Lactam antibiotics do not accumulate in phagocytes, probably because of their acidic character. We therefore synthesized a basic derivative of penicillin G, namely, /sup 14/C-labeled N-(3-dimethylamino-propyl)benzylpenicillinamide (ABP), and studied its uptake and subcellular localization in J774 macrophages compared with that of /sup 14/C-labeled penicillin G. Whereas the intracellular concentration (Ci) of penicillin G remained lower than its extracellular concentration (Ce), ABP reached a Ci/Ce ratio of 4 to 5. Moreover, approximately 50% of intracellular ABP was found associated with lysosomes after isopycnic centrifugation of cell homogenates in isoosmotic Percoll or hyperosmotic sucrose gradients. The behavior of ABP was thus partly consistent with the model of de Duve et al., in which they described the intralysosomal accumulation of weak organic bases in lysosomes. Although ABP is microbiologically inactive, our results show that beta-lactam antibiotics can be driven into cells by appropriate modification. Further efforts therefore may be warranted in the design of active compounds or prodrugs that may prove useful in the chemotherapy of intracellular infections.

  1. Phytotoxicity and accumulation of chromium in carrot plants and the derivation of soil thresholds for Chinese soils.

    PubMed

    Ding, Changfeng; Li, Xiaogang; Zhang, Taolin; Ma, Yibing; Wang, Xingxiang

    2014-10-01

    Soil environmental quality standards in respect of heavy metals for farmlands should be established considering both their effects on crop yield and their accumulation in the edible part. A greenhouse experiment was conducted to investigate the effects of chromium (Cr) on biomass production and Cr accumulation in carrot plants grown in a wide range of soils. The results revealed that carrot yield significantly decreased in 18 of the total 20 soils with Cr addition being the soil environmental quality standard of China. The Cr content of carrot grown in the five soils with pH>8.0 exceeded the maximum allowable level (0.5mgkg(-1)) according to the Chinese General Standard for Contaminants in Foods. The relationship between carrot Cr concentration and soil pH could be well fitted (R(2)=0.70, P<0.0001) by a linear-linear segmented regression model. The addition of Cr to soil influenced carrot yield firstly rather than the food quality. The major soil factors controlling Cr phytotoxicity and the prediction models were further identified and developed using path analysis and stepwise multiple linear regression analysis. Soil Cr thresholds for phytotoxicity meanwhile ensuring food safety were then derived on the condition of 10 percent yield reduction.

  2. Complement (C5)-derived chemotactic activity accounts for accumulation of polymorphonuclear leukocytes in cerebrospinal fluid of rabbits with pneumococcal meningitis.

    PubMed Central

    Ernst, J D; Hartiala, K T; Goldstein, I M; Sande, M A

    1984-01-01

    Experiments were performed to identify the chemoattractant for polymorphonuclear leukocytes that appears in the cerebrospinal fluid of rabbits with experimental pneumococcal meningitis. Meningitis was induced in anesthetized New Zealand white rabbits by injecting 10(4) cells of stationary-phase Streptococcus pneumoniae type III intracisternally. Before bacteria were injected, cerebrospinal fluid contained neither polymorphonuclear leukocytes nor chemotactic activity. Significant chemotactic activity for rabbit polymorphonuclear leukocytes was detected 12 h after inoculation with bacteria and was maximal after 18 to 20 h. Chemotactic activity appeared in cerebrospinal fluid while concentrations of pneumococci and total protein were increasing but before there was any accumulation of polymorphonuclear leukocytes. The chemotactic activity in cerebrospinal fluid was heat stable (56 degrees C for 30 min), eluted from Sephadex G-75 with a profile identical to that of the chemotactic activity in zymosan-activated rabbit serum, and was inhibited by treatment with antibodies to native human C5. In addition, preincubation of polymorphonuclear leukocytes with partially purified rabbit C5a selectively inhibited their subsequent chemotactic responses to cerebrospinal fluid. These data indicate that complement (C5)-derived chemotactic activity appears in cerebrospinal fluid during the course of experimental pneumococcal meningitis in rabbits and suggest that this activity accounts for the accumulation of polymorphonuclear leukocytes observed in this infection. PMID:6480117

  3. Metabolically-Derived Human Ventilation Rates: A Revised Approach Based Upon Oxygen Consumption Rates (External Review Draft)

    EPA Science Inventory

    EPA has released a draft report entitled, Metabolically-Derived Human Ventilation Rates: A Revised Approach Based Upon Oxygen Consumption Rates, for independent external peer review and public comment. NCEA published the Exposure Factors Handbook in 1997. This comprehens...

  4. Growth rate, protein accumulation, and catabolic enzyme activity of skeletal muscles of galliform birds.

    PubMed

    Shea, Russell E; Olson, John M; Ricklefs, Robert E

    2007-01-01

    We measured the mass and several potential indices of functional capacity of the leg and pectoral muscles through 21 d of age in chicks of three species of galliform birds and the domesticated turkey. The study was conducted to test the hypothesis that the growth rate of a tissue is inversely related to its capacity for mature function across species. We measured the proportion of protein and the activities of the catabolic enzymes citrate synthase (CS), pyruvate kinase (PK), and beta -hydroxy-acyl-CoA-dehydrogenase (HOAD) and estimated exponential growth rate (EGR) from growth increments. EGR was negatively related to proportion of protein, PK, and HOAD and positively related to CS activity. In a multiple regression, EGR was uniquely related only to proportion of protein; it was higher in pectoral muscles and increased in this order: wild turkeyrate. When the proportion of protein was normalized by its maximum value for each species and muscle type, the relationship between EGR and normalized protein did not differ significantly among species or muscle type. Thus, if we accept the proportion of protein relative to the mature level as an index of functional capacity--presumably representing the assembly of the contractile apparatus--then growth rate is consistently inversely related to a muscle's capacity for mature function, that is, force generation.

  5. 77 FR 5416 - Financial Derivatives Transactions To Offset Interest Rate Risk; Investment and Deposit Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-03

    ... in certain derivatives transactions for the purpose of offsetting interest rate risk (IRR).\\1\\ This... permit FCUs to enter derivatives transactions for the purpose of offsetting IRR. It now seeks additional... engage in derivatives to offset IRR. In ANPR I, the Board sought comment on whether it should allow...

  6. Metabolically Derived human ventilation rates: A revised approach based upon oxygen consumption rates (Final Report) 2009

    EPA Science Inventory

    The purpose of this report is to provide a revised approach for calculating an individual's ventilation rate directly from their oxygen consumption rate. This revised approach will be used to update the ventilation rate information in the Exposure Factors Handbook, which serve as...

  7. Dickkopf-related protein 1 (Dkk1) regulates the accumulation and function of myeloid derived suppressor cells in cancer.

    PubMed

    D'Amico, Lucia; Mahajan, Sahil; Capietto, Aude-Hélène; Yang, Zhengfeng; Zamani, Ali; Ricci, Biancamaria; Bumpass, David B; Meyer, Melissa; Su, Xinming; Wang-Gillam, Andrea; Weilbaecher, Katherine; Stewart, Sheila A; DeNardo, David G; Faccio, Roberta

    2016-05-02

    Tumor-stroma interactions contribute to tumorigenesis. Tumor cells can educate the stroma at primary and distant sites to facilitate the recruitment of heterogeneous populations of immature myeloid cells, known as myeloid-derived suppressor cells (MDSCs). MDSCs suppress T cell responses and promote tumor proliferation. One outstanding question is how the local and distant stroma modulate MDSCs during tumor progression. Down-regulation of β-catenin is critical for MDSC accumulation and immune suppressive functions in mice and humans. Here, we demonstrate that stroma-derived Dickkopf-1 (Dkk1) targets β-catenin in MDSCs, thus exerting immune suppressive effects during tumor progression. Mice bearing extraskeletal tumors show significantly elevated levels of Dkk1 in bone microenvironment relative to tumor site. Strikingly, Dkk1 neutralization decreases tumor growth and MDSC numbers by rescuing β-catenin in these cells and restores T cell recruitment at the tumor site. Recombinant Dkk1 suppresses β-catenin target genes in MDSCs from mice and humans and anti-Dkk1 loses its antitumor effects in mice lacking β-catenin in myeloid cells or after depletion of MDSCs, demonstrating that Dkk1 directly targets MDSCs. Furthermore, we find a correlation between CD15(+) myeloid cells and Dkk1 in pancreatic cancer patients. We establish a novel immunomodulatory role for Dkk1 in regulating tumor-induced immune suppression via targeting β-catenin in MDSCs.

  8. Comparing infrared star formation rate indicators with optically derived quantities

    NASA Astrophysics Data System (ADS)

    Young, J. E.; Gronwall, C.; Salzer, J. J.; Rosenberg, J. L.

    2014-09-01

    We examine the UV reprocessing efficiencies of warm dust and polycyclic aromatic hydrocarbons (PAHs) through an analysis of the mid- and far-infrared surface luminosity densities of 85 nearby Hα-selected star-forming galaxies detected by the volume-limited KPNO (Kitt Peak National Observatory) International Spectroscopic Survey (KISS). Because Hα selection is not biased towards continuum-bright objects, the KISS sample spans a wide range in stellar masses (108-1012 M⊙), as well as Hα luminosity (1039-1043 erg s-1), mid-infrared 8.0 μm luminosity (1041-1044 erg s-1), and [Bw - R] colour (-0.1-2.2). We find that mid-infrared PAH emission in the Spitzer InfraRed Array Camera (IRAC) 8.0 μm band correlates with star formation, and that the efficiency with which galaxies reprocess UV energy into PAH emission depends on metallicity. We also find that the relationship between far-infrared luminosity in the Spitzer Multiband Imaging Photometer for Spitzer 24 μm band pass and Hα-measured star formation rate varies from galaxy to galaxy within our sample; we do not observe a metallicity dependence in this relationship. We use optical colours and established mass-to-light relationships to determine stellar masses for the KISS galaxies; we compare these masses to those of nearby galaxies as a confirmation that the volume-limited nature of KISS avoids strong biases. We also examine the relationship between IRAC 3.6 μm luminosity and galaxy stellar mass, and find a colour-dependent correlation between the two.

  9. Effect of self-alkalization on nitrite accumulation in a high-rate denitrification system: Performance, microflora and enzymatic activities.

    PubMed

    Li, Wei; Shan, Xiao-Yu; Wang, Zhi-Yao; Lin, Xiao-Yu; Li, Chen-Xu; Cai, Chao-Yang; Abbas, Ghulam; Zhang, Meng; Shen, Li-Dong; Hu, Zhi-Qiang; Zhao, He-Ping; Zheng, Ping

    2016-01-01

    The self-alkalization of denitrifying automatic circulation (DAC) reactor resulted in a large increase of pH up to 9.20 and caused a tremendous accumulation of nitrite up to 451.1 ± 49.0 mgN L(-1) at nitrate loading rate (NLR) from 35 kgN m(-3) d(-1) to 55 kgN m(-3) d(-1). The nitrite accumulation was greatly relieved even at the same NLR once the pH was maintained at 7.6 ± 0.2 in the system. Enzymatic assays indicated that the long-term bacterial exposure to high pH significantly inhibited the activity of copper type nitrite reductase (NirK) rather than the cytochrome cd1 type nitrite reductase (NirS). The terminal restriction fragment length polymorphism (T-RFLP) analysis revealed that the dominant denitrifying bacteria shifted from the NirS-containing Thauear sp. 27 to the NirK-containing Hyphomicrobium nitrativorans strain NL23 during the self-alkalization. The significant nitrite accumulation in the high-rate denitrification system could be therefore, due to the inhibition of Cu-containing NirK by high pH from the self-alkalization. The results suggest that the NirK-containing H. nitrativorans strain NL23 could be an ideal functional bacterium for the conversion of nitrate to nitrite, i.e. denitritation, which could be combined with anaerobic ammonium oxidation (Anammox) to develop a new process for nitrogen removal from wastewater.

  10. Rate of denitrification and the accumulation of intermediates in a denitrifying bioreactor

    NASA Astrophysics Data System (ADS)

    Parsignault, D. R.; Gursky, H.; Kellogg, E. M.; Matilsky, T.; Murray, S.; Schreier, E.; Tananbaum, H.; Giacconi, R.; Brinkman, A. C.

    2012-12-01

    Denitrifying bioreactors (DNBRs) are an emerging mechanism to mitigate the impact of excess reactive nitrogen by harnessing the activity of ubiquitous denitrifying soil microbes. DNBRs fundamentally consist of an organic carbon energy source sufficiently saturated to develop anaerobic conditions and support heterotrophic reduction of nitrate to dinitrogen. Although recent research has well established achievable nitrate removal in DNBRs upwards of 90%, few studies experimentally determine the fate of nitrogen in these systems. This study differentiates between denitrification to inert nitrogen gas, which permanently removes reactive nitrogen from an enriched ecosystem, and transformation of nitrate to another bioavailable form (such as N2O or NOX, powerful greenhouse gases). Previous research has failed to make this distinction and as both are perceived as a reduction in nitrate concentration at the outlet, the utility of DNBRs in reducing downstream reactive nitrogen has not been sufficiently established. In order to quantify the rate of nitrate removal and the products produced, dissolved gas samples are collected from the DNBR with passive diffusion gas samplers while the influent and effluent nitrate concentration and chemical oxygen demand are monitored in real time with spectrometer probes. Nitrate removal is compared with the denitrification rate and the ratio of dinitrogen to nitrous oxide is reported. Denitrification is quantified from the proportion of nitrogen gas products produced from the nitrate pool, indicated by the negative congruence of the regression of 15N enrichment in the nitrate pool and temporal depletion in the gaseous products. The proportion of nitrous oxide to dinitrogen is examined with respect to saturation and redox potential. This research informs the interpretation of previous studies as well as advises the focus of long-term system level monitoring that will provide further information on the design and application of DNBRs to

  11. Relationships between salinity and short-term soil carbon accumulation rates form marsh types across a landscape in the Mississippi River Delta

    USGS Publications Warehouse

    Baustian, Melissa M.; Stagg, Camille L.; Perry, Carey L; Moss, Leland C; Carruthers, Tim J.B.; Allison, Mead

    2017-01-01

    Salinity alterations will likely change the plant and environmental characteristics in coastal marshes thereby influencing soil carbon accumulation rates. Coastal Louisiana marshes have been historically classified as fresh, intermediate, brackish, or saline based on resident plant community and position along a salinity gradient. Short-term total carbon accumulation rates were assessed by collecting 10-cm deep soil cores at 24 sites located in marshes spanning the salinity gradient. Bulk density, total carbon content, and the short-term accretion rates obtained with feldspar horizon markers were measured to determine total carbon accumulation rates. Despite some significant differences in soil properties among marsh types, the mean total carbon accumulation rates among marsh types were not significantly different (mean ± std. err. of 190 ± 27 g TC m−2 year−1). However, regression analysis indicated that mean annual surface salinity had a significant negative relationship with total carbon accumulation rates. Based on both analyses, the coastal Louisiana total marsh area (1,433,700 ha) accumulates about 2.7 to 3.3 Tg C year−1. Changing salinities due to increasing relative sea level or resulting from restoration activities may alter carbon accumulation rates in the short term and significantly influence the global carbon cycle.

  12. Understanding the rates of nonpolar organic chemical accumulation into passive samplers deployed in the environment: Guidance for passive sampler deployments.

    PubMed

    Apell, Jennifer N; Tcaciuc, A Patricia; Gschwend, Philip M

    2016-07-01

    Polymeric passive samplers have become a common method for estimating freely dissolved concentrations in environmental media. However, this approach has not yet been adopted by investigators conducting remedial investigations of contaminated environmental sites. Successful adoption of this sampling methodology relies on an understanding of how passive samplers accumulate chemical mass as well as developing guidance for the design and deployment of passive samplers. Herein, we outline the development of a simple mathematical relationship of the environmental, polymer, and chemical properties that control the uptake rate. This relationship, called a timescale, is then used to illustrate how each property controls the rate of equilibration in samplers deployed in the water or in the sediment. Guidance is also given on how to use the timescales to select an appropriate polymer, deployment time, and suite of performance reference compounds. Integr Environ Assess Manag 2016;12:486-492. © 2015 SETAC.

  13. Variations in heavy metal accumulation, growth and yield of rice plants grown at different sewage sludge amendment rates.

    PubMed

    Singh, R P; Agrawal, M

    2010-05-01

    Use of sewage sludge in agriculture is an alternative disposal technique for this waste. The present field study was conducted to assess the suitability of sewage sludge amendment in soil for rice (Oryza sativa L. cv. Pusa sugandha 3) by evaluating the heavy metal accumulation, growth, biomass and yield responses of plants grown at 0, 3, 4.5, 6, 9, 12 kgm(-2) sewage sludge amendment (SSA) rate. Sewage sludge amendment modified the physico-chemical properties of soil, thus increasing the availability of heavy metals in soil and consequently with higher accumulation in plant parts. Root length decreased, whereas shoot length, number of leaves, leaf area and total biomass increased significantly when grown under various SSA rates. Yield of rice increased by 60%, 111%, 125%, 134% and 137% at 3, 4.5, 6, 9 and 12 kgm(-2) SSA, respectively, as compared to those grown in unamended soil. Sewage sludge amendment rates above 4.5 kgm(-2) though increased the yield of rice, but caused risk of food chain contamination as concentrations of Ni and Cd in rice grains were found to be above the Indian safe limits (1.5 mgkg(-1)) of human consumption above 4.5 kgm(-2) SSA and of Pb (2.5 mgkg(-1)) above 6 kgm(-2) SSA. Since aboveground parts of the rice also showed higher concentration than the permissible levels of Ni, Cd and Pb at 4.5 kgm(-2) SSA rate, it cannot be used as fodder. The rice husk may be used as bioresource for energy production. Efforts should be made to treat the effluents from small scale industries before discharge into the sewerage system.

  14. Biogenic silica fluxes and accumulation rates in the Gulf of California

    SciTech Connect

    Thunell, R.C.; Pride, C.J.; Tappa, E. ); Muller-Karger, F.E. )

    1994-04-01

    The Gulf of California, though small in size, plays an important role in the global silica cycle. The seasonal pattern of biogenic silica flux in the gulf is closely related to that of phytoplankton biomass levels and is controlled by changes in weather and hydrographic conditions. The highest opal fluxes ([approximately] 0.35 g[center dot]m[sup [minus]2][center dot]d[sup [minus]1]) occur during winter and spring, and they are comparable to those measured in some of the most productive ecosystems of the world. Approximately 15%-25% of the biogenic silica produced in surface waters is preserved in gulf sediments, a figure significantly higher than the average global ocean preservation rate. However, the flux of opal at 500 m water depth is less than 25% of that being produced at the surface, suggesting that most of the recycling of biogenic silica in the Gulf of California occurs in the upper water column. 28 refs., 3 figs.

  15. Influences of sediment geochemistry on metal accumulation rates and toxicity in the aquatic oligochaete Tubifex tubifex.

    PubMed

    Méndez-Fernández, Leire; De Jonge, Maarten; Bervoets, Lieven

    2014-12-01

    Metal bioaccumulation and toxicity in the aquatic oligochaete Tubifex tubifex exposed to three metal-contaminated field-sediments was studied in order to assess whether sediment-geochemistry (AVS, TOC) plays a major role in influencing these parameters, and to assess if the biodynamic concept can be used to explain observed effects in T. tubifex tissue residues and/or toxicity. An active autotomy promotion was observed in three studied sediments at different time points and reproduction impairment could be inferred in T. tubifex exposed to two of the tested sites after 28 days. The present study showed that sediment metal concentration and tissue residues followed significant regression models for four essential metals (Cu, Co, Ni and Zn) and one non-essential metal (Pb). Organic content normalization for As also showed a significant relationship with As tissue residue. Porewater was also revealed to be an important source of metal uptake for essential metals (e.g. Cu, Ni and Zn) and for As, but AVS content was not relevant for metal uptake in T. tubifex in studied sediments. Under the biodynamic concept, it was shown that influx rate from food (IF, sediment ingestion) in T. tubifex, in a range of sediment geochemistry, was able to predict metal bioaccumulation, especially of the essential metals Cu, Ni and Zn, and for the non-essential metal Pb. Additionally, IF appeared to be a better predictor for metal bioaccumulation in T. tubifex compared to sediment geochemistry normalization.

  16. TCDD induces dermal accumulation of keratinocyte-derived matrix metalloproteinase-10 in an organotypic model of human skin

    SciTech Connect

    De Abrew, K. Nadira; Thomas-Virnig, Christina L.; Rasmussen, Cathy A.; Bolterstein, Elyse A.; Schlosser, Sandy J.; Allen-Hoffmann, B. Lynn

    2014-05-01

    The epidermis of skin is the first line of defense against the environment. A three dimensional model of human skin was used to investigate tissue-specific phenotypes induced by the environmental contaminant, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Continuous treatment of organotypic cultures of human keratinocytes with TCDD resulted in intracellular spaces between keratinocytes of the basal and immediately suprabasal layers as well as thinning of the basement membrane, in addition to the previously reported hyperkeratinization. These tissue remodeling events were preceded temporally by changes in expression of the extracellular matrix degrading enzyme, matrix metalloproteinase-10 (MMP-10). In organotypic cultures MMP-10 mRNA and protein were highly induced following TCDD treatment. Q-PCR and immunoblot results from TCDD-treated monolayer cultures, as well as indirect immunofluorescence and immunoblot analysis of TCDD-treated organotypic cultures, showed that MMP-10 was specifically contributed by the epidermal keratinocytes but not the dermal fibroblasts. Keratinocyte-derived MMP-10 protein accumulated over time in the dermal compartment of organotypic cultures. TCDD-induced epidermal phenotypes in organotypic cultures were attenuated by the keratinocyte-specific expression of tissue inhibitor of metalloproteinase-1, a known inhibitor of MMP-10. These studies suggest that MMP-10 and possibly other MMP-10-activated MMPs are responsible for the phenotypes exhibited in the basement membrane, the basal keratinocyte layer, and the cornified layer of TCDD-treated organotypic cultures. Our studies reveal a novel mechanism by which the epithelial–stromal microenvironment is altered in a tissue-specific manner thereby inducing structural and functional pathology in the interfollicular epidermis of human skin. - Highlights: • TCDD causes hyperkeratosis and basement membrane changes in a model of human skin. • TCDD induces MMP-10 expression in organotypic cultures

  17. Mononuclear phagocyte accumulates a stearic acid derivative during differentiation into macrophages. Effects of stearic acid on macrophage differentiation and Mycobacterium tuberculosis control.

    PubMed

    Mosquera-Restrepo, Sergio Fabián; Caro, Ana Cecilia; Peláez-Jaramillo, Carlos Alberto; Rojas, Mauricio

    2016-05-01

    The fatty acid composition of monocytes changes substantially during differentiation into macrophages, increasing the proportion of saturated fatty acids. These changes prompted us to investigate whether fatty acid accumulation in the extracellular milieu could affect the differentiation of bystander mononuclear phagocytes. An esterified fatty acid derivative, stearate, was the only fatty acid that significantly increased in macrophage supernatants, and there were higher levels when cells differentiated in the presence of Mycobacterium tuberculosis H37Rv or purified protein derivative (PPD). Exogenous stearic acid enhanced the expression of HLA-DR and CD64; there was also accumulation of IL-12, TNF-α, IL-6, MIP-1 α and β and a reduction in MCP-1 and the bacterial load. These results suggested that during differentiation, a derivative of stearic acid, which promotes the process as well as the effector mechanisms of phagocytes against the mycobacterium, accumulates in the cell supernatants.

  18. Effect of sampling rate and record length on the determination of stability and control derivatives

    NASA Technical Reports Server (NTRS)

    Brenner, M. J.; Iliff, K. W.; Whitman, R. K.

    1978-01-01

    Flight data from five aircraft were used to assess the effects of sampling rate and record length reductions on estimates of stability and control derivatives produced by a maximum likelihood estimation method. Derivatives could be extracted from flight data with the maximum likelihood estimation method even if there were considerable reductions in sampling rate and/or record length. Small amplitude pulse maneuvers showed greater degradation of the derivative maneuvers than large amplitude pulse maneuvers when these reductions were made. Reducing the sampling rate was found to be more desirable than reducing the record length as a method of lessening the total computation time required without greatly degrading the quantity of the estimates.

  19. Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73.

    PubMed

    Théry, C; Regnault, A; Garin, J; Wolfers, J; Zitvogel, L; Ricciardi-Castagnoli, P; Raposo, G; Amigorena, S

    1999-11-01

    Exosomes are membrane vesicles secreted by hematopoietic cells upon fusion of late multivesicular endosomes with the plasma membrane. Dendritic cell (DC)-derived exosomes induce potent antitumor immune responses in mice, resulting in the regression of established tumors (Zitvogel, L., A. Regnault, A. Lozier, J. Wolfers, C. Flament, D. Tenza, P. Ricciardi-Castagnoli, G. Raposo, and S. Amigorena. 1998. Nat. Med. 4:594-600). To unravel the molecular basis of exosome-induced immune stimulation, we now analyze the regulation of their production during DC maturation and characterize extensively their protein composition by peptide mass mapping. Exosomes contain several cytosolic proteins (including annexin II, heat shock cognate protein hsc73, and heteromeric G protein Gi2alpha), as well as different integral or peripherally associated membrane proteins (major histocompatibility complex class II, Mac-1 integrin, CD9, milk fat globule-EGF-factor VIII [MFG-E8]). MFG-E8, the major exosomal component, binds integrins expressed by DCs and macrophages, suggesting that it may be involved in exosome targeting to these professional antigen-presenting cells. Another exosome component is hsc73, a cytosolic heat shock protein (hsp) also present in DC endocytic compartments. hsc73 was shown to induce antitumor immune responses in vivo, and therefore could be involved in the exosome's potent antitumor effects. Finally, exosome production is downregulated upon DC maturation, indicating that in vivo, exosomes are produced by immature DCs in peripheral tissues. Thus, DC-derived exosomes accumulate a defined subset of cellular proteins reflecting their endosomal biogenesis and accounting for their biological function.

  20. Biomass Accumulation Rates of Amazonian Secondary Forest and Biomass of Old-Growth Forests from Landsat Time Series and GLAS

    NASA Astrophysics Data System (ADS)

    Helmer, E.; Lefsky, M. A.; Roberts, D.

    2009-12-01

    We estimate the age of humid lowland tropical forests in Rondônia, Brazil, from a somewhat densely spaced time series of Landsat images (1975-2003) with an automated procedure, the Threshold Age Mapping Algorithm (TAMA), first described here. We then estimate a landscape-level rate of aboveground woody biomass accumulation of secondary forest by combining forest age mapping with biomass estimates from the Geoscience Laser Altimeter System (GLAS). Though highly variable, the estimated average biomass accumulation rate of 8.4 Mg ha-1 yr-1 agrees well with ground-based studies for young secondary forests in the region. In isolating the lowland forests, we map land cover and general types of old-growth forests with decision tree classification of Landsat imagery and elevation data. We then estimate aboveground live biomass for seven classes of old-growth forest. TAMA is simple, fast, and self-calibrating. By not using between-date band or index differences or trends, it requires neither image normalization nor atmospheric correction. In addition, it uses an approach to map forest cover for the self-calibrations that is novel to forest mapping with satellite imagery; it maps humid secondary forest that is difficult to distinguish from old-growth forest in single-date imagery; it does not assume that forest age equals time since disturbance; and it incorporates Landsat Multispectral Scanner (MSS) imagery. Variations on the work that we present here can be applied to other forested landscapes. Applications that use image time series will be helped by the free distribution of coregistered Landsat imagery, which began in December 2008, and of the Ice Cloud and land Elevation Satellite (ICESat) Vegetation Product, which simplifies the use of GLAS data. Finally, we demonstrate here for the first time how the optical imagery of fine spatial resolution that is viewable on Google Earth provides a new source of reference data for remote sensing applications related to land cover

  1. Predicting the visitation of carcasses by carrion-related insects under different rates of degree-day accumulation.

    PubMed

    Michaud, Jean-Philippe; Moreau, Gaétan

    2009-03-10

    Common assumptions in forensic entomology are that insects visit and colonize carcasses following a predictable sequence, and that this succession varies among seasons. However, currently available evidence for insect succession on decomposing bodies is essentially descriptive and, to our knowledge, the fine-scale predictability of insect succession with respect to seasons has never been confirmed statistically. In this study, we test these assumptions through the sampling of carrion-related insects attracted to pig carcasses. The study was carried out during the summer and fall of 2006 in rural fields of New Brunswick, Canada. Of the five species of carrion-related insects with high enough occurrence on carcasses to allow modelling, three showed predictable occurrence with respect to degree-day accumulation and seasonal effects. This demonstrates that the occurrence probability of some carrion-related insects on carcasses can be estimated from meteorological records even across seasons with different rates of degree-day accumulation. As opposed to the prevailing idea that adult insects are not reliable for post-mortem interval estimation, the adults of some species exhibited a specific pattern of visitation that could be determined and used in forensic investigations. It is stressed, however, that the statistical predictability of species occurrence must be assessed before any species is considered as a post-mortem interval indicator.

  2. Accumulation of Cd by the marine sponge Halichondria panicea pallas: Effects upon filtration rate and its relevance for biomonitoring

    SciTech Connect

    Olesen, T.M.E.; Weeks, J.M. )

    1994-05-01

    The marine demosponge Halichondria panicea Pallas, is a cosmopolitan species occurring in coastal waters with varied conditions of light, current, salinity and turbidity. H. panicea has a leuconoid structure and is composed of siliceous spicules and spongin fibers. Sponges are important members of many shallow water marine benthic communities, but comparatively little is known of their trace metal biology. Sponge architecture is constructed around a system of water canals and the physiology of the sponge is largely dependent on the currents of water flowing through their bodies. The volume of water pumped by a sponge is remarkable, ca. 100-1200 ml h[sup [minus]1] g[sup [minus]1]. This large volume of water passing through the body of a sponge means that most cells are in direct contact with the external medium. Many sponges are able to accumulate trace metals and are highly tolerant of such pollutants. This has led to the proposal that a [open quotes]sponge watch[close quotes] program be initiated supplementary to the existing [open quotes]mussel watch[close quotes] program. In view of the large volume of water passing through the bodies of sponges such as H. panicea, the suitability of this species as a biomonitoring organism was further investigated. This study describes the accumulation strategy of the demosponge H. panicea exposed to dissolved cadmium (Cd) and the effect of Cd upon sponge filtration rate.

  3. Intermediate filament protein accumulation in motor neurons derived from giant axonal neuropathy iPSCs rescued by restoration of gigaxonin

    PubMed Central

    Johnson-Kerner, Bethany L.; Ahmad, Faizzan S.; Diaz, Alejandro Garcia; Greene, John Palmer; Gray, Steven J.; Samulski, Richard Jude; Chung, Wendy K.; Van Coster, Rudy; Maertens, Paul; Noggle, Scott A.; Henderson, Christopher E.; Wichterle, Hynek

    2015-01-01

    Giant axonal neuropathy (GAN) is a progressive neurodegenerative disease caused by autosomal recessive mutations in the GAN gene resulting in a loss of a ubiquitously expressed protein, gigaxonin. Gene replacement therapy is a promising strategy for treatment of the disease; however, the effectiveness and safety of gigaxonin reintroduction have not been tested in human GAN nerve cells. Here we report the derivation of induced pluripotent stem cells (iPSCs) from three GAN patients with different GAN mutations. Motor neurons differentiated from GAN iPSCs exhibit accumulation of neurofilament (NF-L) and peripherin (PRPH) protein and formation of PRPH aggregates, the key pathological phenotypes observed in patients. Introduction of gigaxonin either using a lentiviral vector or as a stable transgene resulted in normalization of NEFL and PRPH levels in GAN neurons and disappearance of PRPH aggregates. Importantly, overexpression of gigaxonin had no adverse effect on survival of GAN neurons, supporting the feasibility of gene replacement therapy. Our findings demonstrate that GAN iPSCs provide a novel model for studying human GAN neuropathologies and for the development and testing of new therapies in relevant cell types. PMID:25398950

  4. A new mechanism for the regulation of stomatal aperture size in intact leaves: Accumulation of mesophyll-derived sucrose in the guard-cell wall of Vicia faba

    SciTech Connect

    Lu, Ping; Outlaw, W.H. Jr.; Smith, B.G.; Freed, G.A.

    1997-05-01

    At various times after pulse-labeling broad bean (Vicia faba L.) leaflets with {sup 14}CO{sub 2}, whole-leaf pieces and rinsed epidermal peels were harvested and subsequently processed for histochemical analysis. Cells dissected from whole leaf retained apoplastic contents, whereas those from rinsed peels contained only symplastic contents. Sucrose (Suc)-specific radioactivity peaked (111 GBq mol{sup -1}) in palisade cells at 20 min. In contrast, the {sup 14}C content and Suc-specific radioactivity were very low in guard cells for 20 min, implying little CO, incorporation; both then peaked at 40 min. The guard-cell apoplast had a high maximum Suc-specific radioactivity (204 GBq mol{sup -1}) and a high Suc influx rate (0.05 pmol stoma{sup -1} min{sup -1}). These and other comparisons implied the presence of (a) multiple Suc pools in mesophyll cells, M a localized mesophyll-apoplast region that exchanges with phloem and stomata, and mesophyll-derived Suc in guard-cell walls sufficient to diminish stomatal opening by approximately 3 pm. Factors expected to enhance Suc accumulation in guard-cell walls are (a) high transpiration rate, which closes stomata, and N high apoplastic Suc concentration, which is elevated when mesophyll Suc efflux exceeds translocation. Therefore, multiple physiological factors are integrated in the attenuation of stomatal aperture size by this previously unrecognized mechanism. 50 refs., 9 figs.

  5. Belowground carbon balance and carbon accumulation rate in the successional series of monsoon evergreen broad-leaved forest

    USGS Publications Warehouse

    Zhou, G.; Liu, S.; Tang, X.; Ouyang, X.; Zhang, Dongxiao; Liu, J.; Yan, J.; Zhou, C.; Luo, Y.; Guan, L.; Liu, Yajing

    2006-01-01

    The balance, accumulation rate and temporal dynamics of belowground carbon in the successional series of monsoon evergreen broadleaved forest are obtained in this paper, based on long-term observations to the soil organic matter, input and standing biomass of litter and coarse woody debris, and dissolved organic carbon carried in the hydrological process of subtropical climax forest ecosystem—monsoon evergreen broad-leaved forest, and its two successional forests of natural restoration—coniferous and broad-leaved mixed forest and Pinus massoniana forest, as well as data of root biomass obtained once every five years and respiration measurement of soil, litter and coarse woody debris respiration for 1 year. The major results include: the belowground carbon pools of monsoon evergreen broad-leaved forest, coniferous and broad-leaved mixed forest, and Pinus massoniana forest are 23191 ± 2538 g · m−2, 16889 ± 1936 g · m−2 and 12680 ± 1854 g · m−2, respectively, in 2002. Mean annual carbon accumulation rates of the three forest types during the 24a from 1978 to 2002 are 383 ± 97 g · m−2 · a−1, 193 ± 85 g · m−2 · a−1 and 213 ± 86 g · m−2 · a−1, respectively. The belowground carbon pools in the three forest types keep increasing during the observation period, suggesting that belowground carbon pools are carbon sinks to the atmosphere. There are seasonal variations, namely, they are strong carbon sources from April to June, weak carbon sources from July to September; while they are strong carbon sinks from October to November, weak carbon sinks from December to March.

  6. Drinking water boosts food intake rate, body mass increase and fat accumulation in migratory blackcaps (Sylvia atricapilla).

    PubMed

    Tsurim, Ido; Sapir, Nir; Belmaker, Jonathan; Shanni, Itai; Izhaki, Ido; Wojciechowski, Michał S; Karasov, William H; Pinshow, Berry

    2008-05-01

    Fat accumulation by blackcaps (Sylvia atricapilla) is a prerequisite for successful migratory flight in the autumn and has recently been determined to be constrained by availability of drinking water. Birds staging in a fruit-rich Pistacia atlantica plantation that had access to water increased their body mass and fat reserves both faster and to a greater extent than birds deprived of water. We conducted a series of laboratory experiments on birds captured during the autumn migration period in which we tested the hypotheses that drinking water increases food use by easing limitations on the birds' dietary choices and, consequently, feeding and food processing rates, and that the availability of drinking water leads to improved digestion and, therefore, to higher apparent metabolizable energy. Blackcaps were trapped in autumn in the Northern Negev Desert, Israel and transferred to individual cages in the laboratory. Birds were provided with P. atlantica fruit and mealworms, and had either free access to water (controls) or were water-deprived. In experiment 1, in which mealworm availability was restricted, water-deprived birds had a fourfold lower fruit and energy intake rates and, consequently, gained less fat and total mass than control birds. Water availability did not affect food metabolizability. In experiment 2, in which mealworms were provided ad libitum, water availability influenced the birds' diet: water-restricted birds ate more mealworms, while control birds consumed mainly P. atlantica fruit. Further, in experiment 2, fat and mass gain did not differ between the two treatment groups. We conclude that water availability may have important consequences for fat accumulation in migrating birds while they fatten at stopover sites, especially when water-rich food is scarce. Restricted water availability may also impede the blackcap's dietary shift from insectivory to frugivory, a shift probably necessary for successful pre-migratory fattening.

  7. Essentiality Is a Strong Determinant of Protein Rates of Evolution during Mutation Accumulation Experiments in Escherichia coli.

    PubMed

    Alvarez-Ponce, David; Sabater-Muñoz, Beatriz; Toft, Christina; Ruiz-González, Mario X; Fares, Mario A

    2016-09-26

    The Neutral Theory of Molecular Evolution is considered the most powerful theory to understand the evolutionary behavior of proteins. One of the main predictions of this theory is that essential proteins should evolve slower than dispensable ones owing to increased selective constraints. Comparison of genomes of different species, however, has revealed only small differences between the rates of evolution of essential and nonessential proteins. In some analyses, these differences vanish once confounding factors are controlled for, whereas in other cases essentiality seems to have an independent, albeit small, effect. It has been argued that comparing relatively distant genomes may entail a number of limitations. For instance, many of the genes that are dispensable in controlled lab conditions may be essential in some of the conditions faced in nature. Moreover, essentiality can change during evolution, and rates of protein evolution are simultaneously shaped by a variety of factors, whose individual effects are difficult to isolate. Here, we conducted two parallel mutation accumulation experiments in Escherichia coli, during 5,500-5,750 generations, and compared the genomes at different points of the experiments. Our approach (a short-term experiment, under highly controlled conditions) enabled us to overcome many of the limitations of previous studies. We observed that essential proteins evolved substantially slower than nonessential ones during our experiments. Strikingly, rates of protein evolution were only moderately affected by expression level and protein length.

  8. Amazonian Chemical Weathering Rate Derived from Stony Meteorite Finds at Meridiani Planum on Mars

    NASA Astrophysics Data System (ADS)

    Schröder, C.; Bland, P. A.; Golombek, M. P.; Ashley, J. W.; Warner, N. H.; Grant, J. A.

    2016-08-01

    We used the ferric iron content in stony meteorite finds discovered with the MER Opportunity at Meridiani Planum and constrained their exposure age through related surface features to derive, we believe, the first chemical weathering rate for Mars.

  9. Long-term phenological trends, species accumulation rates, aphid traits and climate: five decades of change in migrating aphids.

    PubMed

    Bell, James R; Alderson, Lynda; Izera, Daniela; Kruger, Tracey; Parker, Sue; Pickup, Jon; Shortall, Chris R; Taylor, Mark S; Verrier, Paul; Harrington, Richard

    2015-01-01

    Aphids represent a significant challenge to food production. The Rothamsted Insect Survey (RIS) runs a network of 12·2-m suction-traps throughout the year to collect migrating aphids. In 2014, the RIS celebrated its 50th anniversary. This paper marks that achievement with an extensive spatiotemporal analysis and the provision of the first British annotated checklist of aphids since 1964. Our main aim was to elucidate mechanisms that advance aphid phenology under climate change and explain these using life-history traits. We then highlight emerging pests using accumulation patterns. Linear and nonlinear mixed-effect models estimated the average rate of change per annum and effects of climate on annual counts, first and last flights and length of flight season since 1965. Two climate drivers were used: the accumulated day degrees above 16 °C (ADD16) indicated the potential for migration during the aphid season; the North Atlantic Oscillation (NAO) signalled the severity of the winter before migration took place. All 55 species studied had earlier first flight trends at rate of β = -0·611 ± SE 0·015 days year(-1). Of these species, 49% had earlier last flights, but the average species effect appeared relatively stationary (β = -0·010 ± SE 0·022 days year(-1)). Most species (85%) showed increasing duration of their flight season (β = 0·336 ± SE 0·026 days year(-1)), even though only 54% increased their log annual count (β = 0·002 ± SE <0·001 year(-1)). The ADD16 and NAO were shown to drive patterns in aphid phenology in a spatiotemporal context. Early in the year when the first aphids were migrating, the effect of the winter NAO was highly significant. Further into the year, ADD16 was a strong predictor. Latitude had a near linear effect on first flights, whereas longitude produced a generally less-clear effect on all responses. Aphids that are anholocyclic (permanently parthenogenetic) or are monoecious (non

  10. 76 FR 37030 - Financial Derivatives Transactions To Offset Interest Rate Risk; Investment and Deposit Activities

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-24

    .... A credit union may enter into a derivatives transaction to protect itself against interest rate risk... obligations associated with non-cleared swaps, including methods used to mitigate credit risk. The information... credit risks associated with derivatives in financial markets. For these reasons, NCUA is...

  11. Smooth Nonparametric Estimation of the Failure Rate Function and its First Two Derivatives

    NASA Astrophysics Data System (ADS)

    Koshkin, G. M.

    2016-10-01

    The class of nonparametric estimators of kernel type is considered for the unknown failure rate function and its derivatives. The convergence of the suggested estimations in distribution and in the mean square sense to the unknown failure rate function and its derivatives is proved. The interval estimator of the failure rate function is constructed. Advantages of the nonparametric estimators in comparison with the parametric algorithms are discussed. The suggested estimators of the failure rate function can be used to solve problems of exploitation reliability of complex physical, technical, and software systems under uncertainty conditions.

  12. Effects of greenhouse warming and N-fertilization on carbon accumulation rates in a nutrient-poor boreal mire: decadal effects assessed using 210Pb

    NASA Astrophysics Data System (ADS)

    Olid, Carolina; Nilsson, Mats B.; Eriksson, Tobias; Klaminder, Jonatan

    2013-04-01

    Boreal peatlands represent a major long-term reservoir of atmospheric carbon (C) and play an important role in the global C cycle. How C accumulation in these peatlands responds to changing temperature and nutrient conditions is under debate. In this study, we assessed how peat and C accumulation rates have responded to increased annual nitrogen additions (30 kg ha-1 yr-1) and increased air temperatures (+3.6°C) in the longest ongoing boreal mire manipulation experiment. Accumulation rates for the uppermost 40 cm of peat in nitrogen and temperature treated plots (n=11) were assessed by 210Pb dating covering the last ~100yrs. A reference surface, installed in 1995 was used as independent validation of the dating model. An empirically based model of organic matter accumulation/degradation was applied to evaluate changes in both peat inputs and organic matter decay rates in response to the treatments. A significant increase in C-accumulation (15 g C m-2 yr-1) was observed in peat subjected to nitrogen additions, while greenhouse warming did not seem to significantly affect C-accumulation or decay rates. Based on our findings we argue that C-accumulation in nutrient poor boreal mires is mainly altered in near-surface peat layers (~15 cm) in response to nitrogen additions and that the uppermost peat layers (<40 cm) in nutrient poor boreal peatlands will continue to function as net C-sinks during the first decades of global warming.

  13. Atomic oxygen, atomic hydrogen, and chemical heating rates derived from SABER

    NASA Astrophysics Data System (ADS)

    Mlynczak, M. G.

    The SABER instrument on the TIMED satellite measures the infrared OH airglow at 2.0 um in the terrestrial mesosphere. These measurements are inverted to provide the volume emissions rates of the OH(9-7 + 8-6) bands. These high-lying bands are formed directly upon the reaction of atomic hydrogen and ozone and thus the measured volume emission rate is a direct measure of the rate of reaction. The SABER OH emission rates and the measured SABER ozone are used to derive the concentration of atomic hydrogen in the mesopause region. The emission rate is also a direct measure of the rate of energy deposition due to the reaction of atomic hydrogen and ozone. Rates of chemical heating are then readily derived upon provision of atmospheric temperature and density from SABER. Under the assumption of photochemical steady state in the production and loss of ozone, the emission rates can also be used to derive atomic oxygen. The abundances of H and O enable the computation of rates of chemical heating due to numerous exothermic reactions. A key to these derivations lies in the knowledge of the rate of quenching/reaction of vibrationally excited OH with atomic oxygen. We present the SABER airglow models, data inversion approach, and results for O, H, and chemical heating.

  14. Exogenous application of pectin-derived oligosaccharides to grape berries modifies anthocyanin accumulation, composition and gene expression.

    PubMed

    Villegas, Daniel; Handford, Michael; Alcalde, José Antonio; Perez-Donoso, Alonso

    2016-07-01

    Anthocyanins are secondary metabolites synthesized in grape berry skins via the phenylpropanoid pathway, with functions ranging from skin coloration to protection against pathogens or UV light. Accumulation of these compounds is highly variable depending on genetics, environmental factors and viticultural practices. Besides their biological functions, anthocyanins improve wine quality, as a high anthocyanin content in berries has a positive impact on the color, total phenolic concentration and, ultimately, the price of wine. The present work studies the effect of the pre-veraison application of pectin derived oligosaccharides (PDO) on the synthesis and accumulation of these compounds, and associates the changes observed with the expression of key genes in the phenylpropanoid pathways. To this end, pre-veraison Cabernet Sauvignon bunches were treated with PDO to subsequently determine total anthocyanin content, the anthocyanin profile (by HPLC-DAD) and gene expression (by qRT-PCR), using Ethrel and water treatments for comparison. The results show that PDO were as efficient as Ethrel in generating a significant rise in total anthocyanin content at 30 days after treatment (dat), compared with water treatments (1.32, 1.48 and 1.02 mg e.Mv-3G/g FW respectively) without any undesirable effect on berry size, soluble solids, tartaric acid concentration or pH. In addition, a significant alteration in the anthocyanin profile was observed. Specifically, a significant increase in the relative concentration of malvidin was observed for both PDO and Ethrel treatments, compared with water controls (52.8; 55.0 and 48.3%, respectively), with a significant rise in tri-hydroxylated forms and a fall in di-hydroxylated anthocyanins. The results of gene expression analyses suggest that the increment in total anthocyanin content is related to a short term increase in phenylalanine ammonia-lyase (PAL) expression, mediated by a decrease in MYB4A expression. A longer term increase in UDP

  15. Glacio-chemical study spanning the past 2 kyr on three ice cores from Dronning Maud Land, Antarctica 1. Annually resolved accumulation rates

    NASA Astrophysics Data System (ADS)

    Sommer, S.; Appenzeller, C.; Röthlisberger, R.; Hutterli, M. A.; Stauffer, B.; Wagenbach, D.; Oerter, H.; Wilhelms, F.; Miller, H.; Mulvaney, R.

    2000-12-01

    For the first time, annually resolved accumulation rates have been determined in central Antarctica by means of counting seasonal signals of ammonium, calcium, and sodium. All records, obtained from three intermediate depth ice cores from Dronning Maud Land, East Antarctica, show rather constant accumulation rates throughout the last 9 centuries with mean values of 63, 61, and 44 mm H2Oyr-1 and a typical year-to-year variation of about 30%. For the last few decades, no trend was detected accounting for the high natural variability of all records. A significant weak intersite correlation is apparent only between two cores when the high-frequency part with periods less than 30 years is removed. By analyzing the records in the frequency domain, no persistent periods were found. This suggests that the snow accumulation in this area is mainly influenced by local deposition patterns and may be additionally masked by redistribution of snow due to wind. By comparing accumulation rates over the last 2 millennia a distinct change in the layer thickness in one of the three cores was found, which might be attributed either to an area upstream of the drilling site with lower accumulation rates, or to deposition processes influenced by surface undulations. The missing of a clear correlation between the accumulation rate histories at the three locations is also important for the interpretation of small, short time variations of past precipitation records obtained from deep ice cores.

  16. Inconsistencies between (14)C and short-lived radionuclides-based sediment accumulation rates: Effects of long-term remineralization.

    PubMed

    Baskaran, M; Bianchi, T S; Filley, T R

    2016-09-06

    (14)C is the most widely utilized geochronometer to investigate geological, geochemical and geophysical problems over the past 5 decades. Establishment of precise sedimentation rates is crucial for the reconstruction of paleo-climate, -ecological and - environmental studies when extrapolation of sedimentation rates is utilized for time scales beyond the dating range. However, agreement between short-term and long-term sedimentation rates in anthropogenically unperturbed sediment cores has not been shown. Here we show that the AMS (14)C-based long-term mass accumulation rate (MAR) of an organic-rich (>70%) sediment core from Mud Lake, Florida to be ∼5 times lower than the short-term MAR obtained using (239,240)Pu, (137)Cs and excess (210)Pb ((210)Pbxs). The measured sediment inventories of (210)Pbxs, (137)Cs and (239,240)Pu are comparable to the atmospheric fallout for the sampling site, indicating very little accelerated sediment erosion over the past several decades. Presence of sharp fallout peaks of (239,240)Pu indicates very little sediment mixing. The penetration depths of (137)Cs and (239,240)Pu were found to be much deeper than expected and this is attributed to their post-depositional mobility. MAR calculated using (14)C-ages in successive layers also indicated decreasing MARs with depth, and was reflective of progressive remineralization. Using first-order kinetics, the sediment remineralization rate was found to be 4.4 × 10(-4) y(-1) and propose that over the long-term, remineralization of organic-rich sediment affected the long-term MAR, but not the ratio of (14)C/(12)C. Thus, the MAR and linear sedimentation rate obtained using (14)C (and other isotope-based methods) could be erroneous, although (14)C ages may not be affected by such remineralization. Long-term remineralization rates of organic matter has a direct bearing on the biogeochemical cycling of elements in aqueous systems and mass balance of elements needs to be taken into consideration.

  17. Processes and rates of sediment and wood accumulation in headwater streams of the Oregon Coast Range, USA

    USGS Publications Warehouse

    May, Christine L.; Gresswell, Robert E.

    2003-01-01

    Channels that have been scoured to bedrock by debris flows provide unique opportunities to calculate the rate of sediment and wood accumulation in low-order streams, to understand the temporal succession of channel morphology following disturbance, and to make inferences about processes associated with input and transport of sediment. Dendrochronology was used to estimate the time since the previous debris flow and the time since the last stand-replacement fire in unlogged basins in the central Coast Range of Oregon. Debris flow activity increased 42 per cent above the background rate in the decades immediately following the last wildfire. Changes in wood and sediment storage were quantified for 13 streams that ranged from 4 to 144 years since the previous debris flow. The volume of wood and sediment in the channel, and the length of channel with exposed bedrock, were strongly correlated with the time since the previous debris flow. Wood increased the storage capacity of the channel and trapped the majority of the sediment in these steep headwater streams. In the absence of wood, channels that have been scoured to bedrock by a debris flow may lack the capacity to store sediment and could persist in a bedrock state for an extended period of time. With an adequate supply of wood, low-order channels have the potential of storing large volumes of sediment in the interval between debris flows and can function as one of the dominant storage reservoirs for sediment in mountainous terrain.

  18. In Vitro Validation of Endovascular Doppler-derived Flow Rates in Models of the Cerebral Circulation

    PubMed Central

    McGah, P M; Nerva, J D; Morton, R P; Barbour, M C; Levitt, M R; Mourad, P D; Kim, L J; Aliseda, A

    2015-01-01

    This study presents validation of endovascular Doppler velocimetry-based volumetric flow rate measurements conducted in a pulsatile flow loop simulating conditions in both the internal carotid and basilar artery. In vitro models of cerebral vessels, each containing an aneurysm, were fabricated from patient anatomies extracted from 3D rotational angiography. Flow velocity measurements were collected with three different experimental techniques: an endovascular Doppler wire, Particle Image Velocimetry, and a time-resolved ultrasonic flow meter. Womersley’s theory of pulsatile flow in a cylindrical vessel was used to compute time-resolved volumetric flow rates from the endovascular Doppler velocity. The volumetric flow rates computed from the Doppler measurements were compared to those from the Particle Image Velocimetry profile measurements, and the direct measurements from the ultrasonic flow meter. The study establishes confidence intervals for any systematic or random errors associated with the wire-derived flow rates as benchmarked to the other two modalities. There is an approximately 10% random error in the Doppler-derived peak and time-averaged flow rates. There is a measurable uniform bias, about 15% too low, in the time-averaged Doppler-derived flow rates. There is also a small proportional bias in the peak systolic Doppler-derived flow rates. Potential sources of error are also discussed. PMID:26450643

  19. Steady-State Computation of Constant Rotational Rate Dynamic Stability Derivatives

    NASA Technical Reports Server (NTRS)

    Park, Michael A.; Green, Lawrence L.

    2000-01-01

    Dynamic stability derivatives are essential to predicting the open and closed loop performance, stability, and controllability of aircraft. Computational determination of constant-rate dynamic stability derivatives (derivatives of aircraft forces and moments with respect to constant rotational rates) is currently performed indirectly with finite differencing of multiple time-accurate computational fluid dynamics solutions. Typical time-accurate solutions require excessive amounts of computational time to complete. Formulating Navier-Stokes (N-S) equations in a rotating noninertial reference frame and applying an automatic differentiation tool to the modified code has the potential for directly computing these derivatives with a single, much faster steady-state calculation. The ability to rapidly determine static and dynamic stability derivatives by computational methods can benefit multidisciplinary design methodologies and reduce dependency on wind tunnel measurements. The CFL3D thin-layer N-S computational fluid dynamics code was modified for this study to allow calculations on complex three-dimensional configurations with constant rotation rate components in all three axes. These CFL3D modifications also have direct application to rotorcraft and turbomachinery analyses. The modified CFL3D steady-state calculation is a new capability that showed excellent agreement with results calculated by a similar formulation. The application of automatic differentiation to CFL3D allows the static stability and body-axis rate derivatives to be calculated quickly and exactly.

  20. MicroRNA-494 is required for the accumulation and functions of tumor-expanded myeloid-derived suppressor cells via targeting of PTEN.

    PubMed

    Liu, Yang; Lai, Lihua; Chen, Qingyun; Song, Yinjing; Xu, Sheng; Ma, Feng; Wang, Xiaojian; Wang, Jianli; Yu, Hai; Cao, Xuetao; Wang, Qingqing

    2012-06-01

    Myeloid-derived suppressor cells (MDSCs) potently suppress the anti-tumor immune responses and also orchestrate the tumor microenvironment that favors tumor angiogenesis and metastasis. The molecular networks regulating the accumulation and functions of tumor-expanded MDSCs are largely unknown. In this study, we identified microRNA-494 (miR-494), whose expression was dramatically induced by tumor-derived factors, as an essential player in regulating the accumulation and activity of MDSCs by targeting of phosphatase and tensin homolog (PTEN) and activation of the Akt pathway. TGF-β1 was found to be the main tumor-derived factor responsible for the upregulation of miR-494 in MDSCs. Expression of miR-494 not only enhanced CXCR4-mediated MDSC chemotaxis but also altered the intrinsic apoptotic/survival signal by targeting of PTEN, thus contributing to the accumulation of MDSCs in tumor tissues. Consequently, downregulation of PTEN resulted in increased activity of the Akt pathway and the subsequent upregulation of MMPs for facilitation of tumor cell invasion and metastasis. Knockdown of miR-494 significantly reversed the activity of MDSCs and inhibited the tumor growth and metastasis of 4T1 murine breast cancer in vivo. Collectively, our findings reveal that TGF-β1-induced miR-494 expression in MDSCs plays a critical role in the molecular events governing the accumulation and functions of tumor-expanded MDSCs and might be identified as a potential target in cancer therapy.

  1. Recent peat accumulation rates in minerotrophic peatlands of the Bay James region, Eastern Canada, inferred by 210Pb and 137Cs radiometric techniques.

    PubMed

    Ali, Adam A; Ghaleb, Bassam; Garneau, Michelle; Asnong, Hans; Loisel, Julie

    2008-10-01

    (210)Pb and (137)Cs dating techniques are used to characterise recent peat accumulation rates of two minerotrophic peatlands located in the La Grande Rivière hydrological watershed, in the James Bay region (Canada). Several cores were collected during the summer 2005 in different parts of the two selected peatlands. These minerotrophic patterned peatlands are presently affected by erosion processes, expressed by progressive mechanical destruction of their pools borders. This erosion process is related to a water table rise induced by a regional increase of humidity since the last century. The main objective of the present paper is to (1) evaluate if (210)Pb and (137)Cs dating techniques can be applied to build accurate chronologies in these environments and (2) detect changes in the peat accumulation rates in regard to this amplification of humidity. In both sites, unsupported (210)Pb shows an exponential decreasing according to the depth. Chronologies inferred from (210)Pb allow to reconstruct peat accumulation rates since ca. 1855 AD. The (137)Cs data displayed evident mobility and diffusion, preventing the establishment of any sustained chronology based on these measurements. In the two sites, peat accumulation rates inferred from (210)Pb chronologies fluctuate between 0.005 and 0.038 g cm(-2) yr(-1). As a result, the rise of the water table during the last decade has not yet affected peat accumulation rates.

  2. Simulation of continental basin margin sedimentation in response to crustal movements, eustatic sea level change, and sediment accumulation rates

    SciTech Connect

    Helland-Hansen, W.; Kendall, C.G.St.C.; Lerche, I.; Nakayama, K.

    1988-10-01

    As eustasy, subsidence, and sediment accumulation vary, a 2D computer-based graphical simulation generates on-lapping and off-lapping geometries of both marine and near coastal alluvial deposits, reproducing timelines within sediment-bodies at basin margins. In the simulation, deposition is expressed by creation of new surfaces above previous ones. Thicknesses of layers are reduced by both erosion and compaction while their surfaces move vertically in response to tectonic change and loading. Simulation is divided into a series of equal time steps in which sediment is deposited as an array of en-echelon columns that mark the top of the previous depositional surface. The volume of sediment deposited in each time step is expressed as a 2D cross section and is derived from two right-angle triangles (sand and shale), whose areas are a 2D expression of the quantity of sediment deposited at that time step and whose length matches the width of the offshore sediment wedge seaward of the shoreline. Each column in the array is filled by both marine sediments up to sea level, and alluvial sediments to a surface determined by an alluvial angle that is projected landward from the shore to its intersection with the previous surface. Each time the area representing the sediment column is subtracted from the triangles, the triangle heights are reduced correspondingly. This process is repeated until the triangle heights match the position of sea level above the sediment surface, at which time the remaining area of the sediment triangle is deposited seaward as a single wedge of offshore sediments. This simulation is designed to aid interpretation of stratigraphic sequences. It can be used as a complement to seismic stratigraphy or can be used alone as an inexpensive test of stratigraphic models.

  3. Modern sediment characteristics and accumulation rates from the delta front to prodelta of the Yellow River (Huanghe)

    NASA Astrophysics Data System (ADS)

    Zhou, Liangyong; Liu, Jian; Saito, Yoshiki; Gao, Maosheng; Diao, Shaobo; Qiu, Jiandong; Pei, Shaofeng

    2016-08-01

    Since 1976, the main channel of the Yellow River (Huanghe) has been on the east side of the delta complex, and the river has prograded a broad new delta lobe in Laizhou Bay of the Bohai Sea. In 2012, extensive bathymetric and high-resolution seismic profiles were conducted and sediment cores were collected off the new delta lobe. This study examined delta sedimentation and morphology along a profile across the modern subaqueous Yellow River delta and into Laizhou Bay, by analyzing sediment radionuclides (137Cs, 210Pb and 7Be), sedimentary structure, grain-size composition, organic carbon content, and morphological changes between 1976 and 2012. The change in the bathymetric profile, longitudinal to the river's course, reveals subaqueous delta progradation during this period. The subbottom boundary between the new delta lobe sediment and the older seafloor sediment (before the 1976 course shift) was identified in terms of lithology and radionuclide distributions, and recognized as a downlap surface in the seismic record. The accumulation rate of the new delta lobe sediment is estimated to be 5-18.6 cm year-1 on the delta front slope, 2 cm year-1 at the toe of the slope, and 1-2 cm year-1 in the shelf areas of Laizhou Bay. Sediment facies also change offshore, from alternations of gray and brown sediment in the proximal area to gray bioturbated fine sediment in the distal area. Based on 7Be distribution, the shorter-term deposition rate was at least 20 cm year-1 in the delta front.

  4. Export production fluctuations in the eastern equatorial Pacific during the Pliocene-Pleistocene: Reconstruction using barite accumulation rates

    NASA Astrophysics Data System (ADS)

    Ma, Zhongwu; Ravelo, Ana Christina; Liu, Zhonghui; Zhou, Liping; Paytan, Adina

    2015-11-01

    Export production is an important component of the carbon cycle, modulating the climate system by transferring CO2 from the atmosphere to the deep ocean via the biological pump. Here we use barite accumulation rates to reconstruct export production in the eastern equatorial Pacific over the past 4.3 Ma. We find that export production fluctuated considerably on multiple time scales. Export production was on average higher (51 g C m-2 yr-1) during the Pliocene than the Pleistocene (40 g C m-2 yr-1), decreasing between 3 and 1 Ma (from more than 60 to 20 g C m-2 yr-1) followed by an increase over the last million years. These trends likely reflect basin-scale changes in nutrient inventory and ocean circulation. Our record reveals decoupling between export production and temperatures on these long (million years) time scale. On orbital time scales, export production was generally higher during cold periods (glacial maxima) between 4.3 and 1.1 Ma. This could be due to stronger wind stress and higher upwelling rates during glacial periods. A shift in the timing of maximum export production to deglaciations is seen in the last ~1.1 million years. Results from this study suggest that, in the eastern equatorial Pacific, mechanisms that affect nutrient supply and/or ecosystem structure and in turn carbon export on orbital time scales differ from those operating on longer time scales and that processes linking export production and climate-modulated oceanic conditions changed about 1.1 million years ago. These observations should be accounted for in climate models to ensure better predictions of future climate change.

  5. Mauna Loa lava accumulation rates at the Hilo drill site: Formation of lava deltas during a period of declining overall volcanic growth

    USGS Publications Warehouse

    Lipman, P.W.; Moore, J.G.

    1996-01-01

    Accumulation rates for lava flows erupted from Mauna Loa, as sampled in the uppermost 280 m of the Hilo drill hole, vary widely for short time intervals (several thousand years), but overall are broadly similar to those documented elsewhere on this volcano since 100 ka. Thickness variations and accumulation rates for Mauna Loa lavas at the Hilo drill site have been strongly affected by local paleotopography, including funneling and ponding between Mauna Kea and Kilauea. In addition, gentle submerged slopes of Mauna Kea in Hilo Bay have permitted large shoreline displacements by Mauna Loa flows. Ages of eruptive intervals have been determined from published isotopic data and from eustatic sea level curves modified to include the isostatic subsidence of the island of Hawaii at 2.2-2.6 mm/yr. Prior to 10 ka, rates of Mauna Loa lava accumulation at the drill site varied from 0.6 to 4.3 mm/yr for dateable intervals, with an overall rate of 1.8 mm/yr. Major eruptive pulses at about 1.3 and 10 ka, each probably representing a single long-lived eruption based on lack of weathering between flow units, increase the overall accumulation rate to 2.4 mm/yr. The higher rate since 10 ka reflects construction of thick near-shoreline lava deltas as postglacial sea levels rose rapidly. Large lava deltas form only along coastal segments where initially subaerial slopes have been submerged by the combined effects of eustatic sea level rise, isostatic subsidence, or spreading of volcano flanks. Overall accumulation of 239 m of lava at the drill site since 100-120 ka closely balances submergence of the Hilo area, suggesting that processes of coastal lava deposition have been modulated by rise in sea level. The Hilo accumulation rate is slightly higher than average rates of 1-2 mm/yr determined elsewhere along the Mauna Loa coast, based on rates of shoreline coverage and dated sea cliff and fault scarp exposures. Low rates of coastal lava accumulation since 100 ka, near or below the rate

  6. Forest dynamics and tip-up pools drive pulses of high carbon accumulation rates in a tropical peat dome in Borneo (Southeast Asia)

    NASA Astrophysics Data System (ADS)

    Dommain, René; Cobb, Alexander R.; Joosten, Hans; Glaser, Paul H.; Chua, Amy F. L.; Gandois, Laure; Kai, Fuu-Ming; Noren, Anders; Salim, Kamariah A.; Su'ut, N. Salihah H.; Harvey, Charles F.

    2015-04-01

    Peatlands of Southeast Asia store large pools of carbon but the mechanisms of peat accumulation in tropical forests remain to be resolved. Patch dynamics and forest disturbance have seldom been considered as drivers that can amplify or dampen rates of peat accumulation. Here we used a modified piston corer, noninvasive geophysical measurements, and geochemical and paleobotanical techniques to establish the effect of tree fall on carbon accumulation rates in a peat swamp forest dominated by Shorea albida in Brunei (Borneo). Carbon initially accumulated in a mangrove forest at over 300 g C m-2 yr-1 but declined to less than 50 g C m-2 yr-1 with the establishment of a peat swamp forest. A rapid accumulation pulse of 720-960 g C m-2 yr-1 occurred around 1080 years ago as a tip-up pool infilled. Tip-up pools are common in the peatlands of northwest Borneo where windthrow and lightning strikes produce tree falls at a rate of 4 trees ha-1 every decade. A simulation model indicates that tip-up pools, which are formed across the entire forested peat dome, produce local discontinuities in the peat deposit, when peat is removed to create a pool that is rapidly filled with younger material. The resulting discontinuities in peat age at the base and sides of pool deposits obscure linkages between carbon accumulation rates and climate and require new approaches for paleoenvironmental reconstructions. Our results suggest that carbon accumulation in tropical peat swamps may be based on fundamentally different peat-forming processes than those of northern peatlands.

  7. Comparison of 1-min rain rate derived from TRMM satellite data and raingauge data for microwave applications in Nigeria

    NASA Astrophysics Data System (ADS)

    Ojo, J. S.; Omotosho, T. V.

    2013-09-01

    A comparative statistical study of derived 1-min rain rate using data obtained from Tropical Rainfall Measuring Mission (TRMM) satellite (3A12 V6 and 3B43V6) and raingauge measurements are presented. 30-years data of rainfall intensities obtained from the Nigerian Meteorological Agency rain-gauge network and 9-years rainfall data obtained from TRMM satellite are used. Cumulative distributions of rain rate were obtained using a combination of an improved version of the Moupfouma-Martin model for rain rate prediction in the tropical regions. Variations of rain rate of different integration time are compared for 28 stations across Nigeria. The Kriging interpolation method was used for spatial interpolation of rain accumulation values into a regular grid in order to obtain a highly consistent and predictable inter-gauge rainfall variation. Noticeable differences between rain rate estimated from rain-gauge and TRMM data were found to be a function of period and location. From quantitative comparison over each of the region, there are reasonably positive correlations between the two sets of data for different percentages of time with correlation coefficient varying from 0.84 to 0.99 at 99.99% availability of time. Comparison with ITU-R 837-5 shows that, ITU model underestimates rain rate as high as 51% from the mean value depending on the percentages of at which time rain rate is exceeded. The overall result shows that the gauge data perform better than TRMM data in most of the sites located in the SW, SE, SS, MB and NE regions, while TRMM data provided better results for sites in the NW region from the statistical perspective with the lower RMS values.

  8. Deriving bioconcentration factors and somatic biotransformation rates from dietary bioaccumulation and depuration tests.

    PubMed

    Gobas, Frank A P C; Lo, Justin C

    2016-12-01

    The present study develops, applies, and tests a method for deriving empirical bioconcentration factors and somatic biotransformation rate constants from dietary bioaccumulation tests and simplified bioaccumulation experiments that measure depuration rates. In this approach, measurement of the chemical concentration in the water is not required. The method aims to improve bioaccumulation assessment, reduce cost and animal use, and shorten experiments. Environ Toxicol Chem 2016;35:2968-2976. © 2016 SETAC.

  9. Kernel-Correlated Levy Field Driven Forward Rate and Application to Derivative Pricing

    SciTech Connect

    Bo Lijun; Wang Yongjin; Yang Xuewei

    2013-08-01

    We propose a term structure of forward rates driven by a kernel-correlated Levy random field under the HJM framework. The kernel-correlated Levy random field is composed of a kernel-correlated Gaussian random field and a centered Poisson random measure. We shall give a criterion to preclude arbitrage under the risk-neutral pricing measure. As applications, an interest rate derivative with general payoff functional is priced under this pricing measure.

  10. The development of frost tolerance and DHN5 protein accumulation in barley (Hordeum vulgare) doubled haploid lines derived from Atlas 68 x Igri cross during cold acclimation.

    PubMed

    Kosová, Klára; Tom Prásil, Ilja; Prásilová, Pavla; Vítámvás, Pavel; Chrpová, Jana

    2010-03-15

    The dynamics of a long-term cold acclimation (CA) was studied in spring barley cultivar Atlas 68, winter barley cultivar Igri and a set of doubled haploid (DH) lines derived from an Atlas 68xIgri cross. The aim was to evaluate the effect of plant development on the ability to induce frost tolerance (FT) and to accumulate dehydrin 5 (DHN5) during CA. The plant developmental stage was evaluated by phenological development of the shoot apex and by determination of days to heading after a certain period of CA. FT was determined by direct frost tests. Plant winter survival was also determined. DHN5 was evaluated by densitometric analysis of protein gel blots. Cold led to the induction of increased FT and to the accumulation of DHN5 in both spring and winter lines. However, with the progression of CA, differences between the growth habits occurred as the winter lines were able to maintain increased FT and DHN5 levels for a significantly longer period of time than the spring lines. After vegetative/reproductive transition, a significant decrease in DHN5 accumulation was found in all lines; however, a discrepancy between the acquired FT level and DHN5 accumulation in vernalized winter barley plants was found. A correlation between DHN5 accumulation and plant winter survival was found when the studied lines were differentiated according to their developmental stage and DHN5 level. Possible explanations for these phenomena are provided.

  11. Bone Marrow-Derived Cell Accumulation in the Spinal Cord Is Independent of Peripheral Mobilization in a Mouse Model of Amyotrophic Lateral Sclerosis

    PubMed Central

    Peake, Kyle; Manning, John; Lewis, Coral-Ann; Tran, Kevin; Rossi, Fabio; Krieger, Charles

    2017-01-01

    Bone marrow-derived cells (BMDCs) are capable of migrating across the blood–brain barrier (BBB) and accumulating in the central nervous system (CNS) when transplanted into recipients conditioned with whole-body irradiation or chemotherapy. We used the chemotherapeutic agents busulfan and treosulfan to condition recipient mice for transplantation with bone marrow (BM) cells isolated from donor mice ubiquitously expressing green fluorescent protein. We attempted to increase the accumulation of BMDCs in the CNS by mobilization of BMDCs using either, or both, granulocyte colony-stimulating factor (GCSF) or plerixafor (AMD3100). We also used several concentrations of busulfan. We hypothesized that higher concentrations of busulfan and BMDC mobilization would increase numbers of GFP+ cells in the CNS. The doses of busulfan employed (60–125 mg/kg) all resulted in high levels of sustained chimerism (>85% 1 year post-transplant) in both the blood and BM of wild-type (WT) mice and an amyotrophic lateral sclerosis (ALS) mouse model. Moreover, cells accumulated within the CNS in a dose-, time-, and disease-dependent manner. Conditioning with the hydrophilic busulfan analog treosulfan, which is unable to cross the BBB efficiently, also resulted in a high degree of BM chimerism. However, few GFP+ BMDCs were found within the CNS of WT or ALS mice of treosulfan-conditioned mice. Mobilization of BMDCs into the circulation using GCSF and/or AMD3100 did not lead to increased accumulation of GFP+ BMDCs within the CNS of WT or ALS mice. Weekly analysis of BMDC accumulation revealed that BMDCs accumulated more rapidly and to a greater extent in the CNS of ALS mice conditioned with a high dose (125 mg/kg) of busulfan compared to a lower dose (80 mg/kg). The number of GFP+ BMDCs in the CNS labeling with the proliferation marker Ki67 increased in parallel with BMDC accumulation within the CNS. Our results indicate that establishment of high levels of blood and BM chimerism

  12. Influence of the rate of ethanol production and accumulation on the viability of Saccharomyces cerevisiae in "rapid fermentation".

    PubMed

    Nagodawithana, T W; Steinkraus, K H

    1976-02-01

    Whereas "rapid fermentation" of diluted clover honey (25 degrees Brix) fortified with yeast nutrients using 8 X 10(8) brewers' yeast cells per ml resulted in an ethanol content of 9.5% (wt/vol; 12% vol/vol) in 3 h at 30 C, death rate of the yeast cells during this period was essentially logarithmic. Whereas 6 h was required to reach the same ethanol content at 15 C, the yeast cells retained their viability. Using a lower cell population (6 X 10(7) cells/ml), a level at which the fermentation was no longer "rapid," the yeast cells also retained their viability at 30 C. Ethanol added to the medium was much less lethal than the same or less quantities of ethanol produced by the cell in "rapid fermentation." It was considered possible that ethanol was produced so rapidly at 30 C that it could not diffuse out of the cell as rapidly as it was formed. The hypothesis was postulated that ethanol accumulating in the cell was contributing to the high death rate at 30 C. It was found that the intracellular ethanol concentration reached a level of approximately 2 X 10(11) ethanol molecules/cell in the first 30 min of fermentation at 30 C. At 15 C, with the same cell count, intracellular ethanol concentration reached a level of approximately 4 X 10(10) ethanol molecules/cell and viability remained high. Also, at 30 C with a lower cell population (6 X 10(7) cells/ml), under which conditions fermentation was no longer "rapid," intracellular ethanol concentration reached a similar level (4 X 10(10) molecules ethanol/cell) and the cells retained their viability. Alcohol dehydrogenase (ADH) lost its activity in brewers' yeast under conditions of "rapid fermentation" at 30 C but retained its activity in cells under similar conditions at 15 C. ADH activity was also retained in fermentations at 30 C with cell populations of 6 X 10(7)/ml. It would appear that an intracellular level of about 5 X 10(10) ethanol molecules/cell is normal and that this level does not damage either cell

  13. Water filtration rate and infiltration/accumulation of low density lipoproteins in 3 different modes of endothelial/smooth muscle cell co-cultures.

    PubMed

    Ding, ZuFeng; Fan, YuBo; Deng, XiaoYan

    2009-11-01

    Using different endothelial/smooth muscle cell co-culture modes to simulate the intimal structure of blood vessels, the water filtration rate and the infiltration/accumulation of LDL of the cultured cell layers were studied. The three cell culture modes of the study were: (i) The endothelial cell monolayer (EC/Phi); (ii) endothelial cells directly co-cultured on the smooth muscle cell monolayer (EC-SMC); (iii) endothelial cells and smooth muscle cells cultured on different sides of a Millicell-CM membrane (EC/SMC). It was found that under the same condition, the water filtration rate was the lowest for the EC/SMC mode and the highest for the EC/Phi mode, while the infiltration/accumulation of DiI-LDLs was the lowest in the EC/Phi mode and the highest in the EC-SMC mode. It was also found that DiI-LDL infiltration/accumulation in the cultured cell layers increased with the increasing water filtration rate. The results from the in vitro model study therefore suggest that the infiltration/accumulation of the lipids within the arterial wall is positively correlated with concentration polarization of atherogenic lipids, and the integrity of the endothelium plays an important role in the penetration and accumulation of atherogenic lipids in blood vessel walls.

  14. Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes

    PubMed Central

    Eng, George; Lee, Benjamin W.; Protas, Lev; Gagliardi, Mark; Brown, Kristy; Kass, Robert S.; Keller, Gordon; Robinson, Richard B.; Vunjak-Novakovic, Gordana

    2016-01-01

    The therapeutic success of human stem cell-derived cardiomyocytes critically depends on their ability to respond to and integrate with the surrounding electromechanical environment. Currently, the immaturity of human cardiomyocytes derived from stem cells limits their utility for regenerative medicine and biological research. We hypothesize that biomimetic electrical signals regulate the intrinsic beating properties of cardiomyocytes. Here we show that electrical conditioning of human stem cell-derived cardiomyocytes in three-dimensional culture promotes cardiomyocyte maturation, alters their automaticity and enhances connexin expression. Cardiomyocytes adapt their autonomous beating rate to the frequency at which they were stimulated, an effect mediated by the emergence of a rapidly depolarizing cell population, and the expression of hERG. This rate-adaptive behaviour is long lasting and transferable to the surrounding cardiomyocytes. Thus, electrical conditioning may be used to promote cardiomyocyte maturation and establish their automaticity, with implications for cell-based reduction of arrhythmia during heart regeneration. PMID:26785135

  15. Rates of post-fire vegetation recovery and fuel accumulation as a function of burn severity and time-since-burn in four western U.S. ecosystems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vegetation recovery and fuel accumulation rates following wildfire are useful measures of ecosystem resilience, yet few studies have quantified these variables over 10 years post-fire. Conventional wisdom is that recovery time to pre-fire condition will be slower as a function of burn severity, as i...

  16. Inhibition of Morganella morganii Histidine Decarboxylase Activity and Histamine Accumulation in Mackerel Muscle Derived from Filipendula ulumaria Extracts.

    PubMed

    Nitta, Yoko; Yasukata, Fumiko; Kitamoto, Noritoshi; Ito, Mikiko; Sakaue, Motoyoshi; Kikuzaki, Hiroe; Ueno, Hiroshi

    2016-03-01

    Filipendula ulmaria, also known as meadowsweet, is an herb; its extract was examined for the prevention of histamine production, primarily that caused by contaminated fish. The efficacy of meadowsweet was assessed using two parameters: inhibition of Morganella morganii histidine decarboxylase (HDC) and inhibition of histamine accumulation in mackerel. Ellagitannins from F. ulmaria (rugosin D, rugosin A methyl ester, tellimagrandin II, and rugosin A) were previously shown to be potent inhibitors of human HDC; and in the present work, these compounds inhibited M. morganii HDC, with half maximal inhibitory concentration values of 1.5, 4.4, 6.1, and 6.8 μM, respectively. Application of the extracts (at 2 wt%) to mackerel meat yielded significantly decreased histamine accumulation compared with treatment with phosphate-buffered saline as a control. Hence, F. ulmaria exhibits inhibitory activity against bacterial HDC and might be effective for preventing food poisoning caused by histamine.

  17. Respiratory rate derived from smartphone-camera-acquired pulse photoplethysmographic signals.

    PubMed

    Lázaro, Jesús; Nam, Yunyoung; Gil, Eduardo; Laguna, Pablo; Chon, Ki H

    2015-11-01

    A method for deriving respiratory rate from smartphone-camera-acquired pulse photoplethysmographic (SCPPG) signal is presented. Our method exploits respiratory information by examining the pulse wave velocity and dispersion from the SCPPG waveform and we term these indices as the pulse width variability (PWV). A method to combine information from several derived respiration signals is also presented and it is used to combine PWV information with other methods such as pulse amplitude variability (PAV), pulse rate variability (PRV), and respiration-induced amplitude and frequency modulations (AM and FM) in SCPPG signals.Evaluation is performed on a database containing SCPPG signals recorded from 30 subjects during controlled respiration experiments at rates from 0.2 to 0.6 Hz with an increment of 0.1 Hz, using three different devices: iPhone 4S, iPod 5, and HTC One M8. Results suggest that spontaneous respiratory rates (0.2-0.4 Hz) can be estimated from SCPPG signals by the PWV- and PRV-based methods with low relative error (median of order 0.5% and interquartile range of order 2.5%). The accuracy can be improved by combining PWV and PRV with other methods such as PAV, AM and/or FM methods. Combination of these methods yielded low relative error for normal respiratory rates, and maintained good performance at higher rates (0.5-0.6 Hz) when using the iPhone 4S or iPod 5 devices.

  18. Accumulation of argpyrimidine, a methylglyoxal-derived advanced glycation end product, increases apoptosis of lens epithelial cells both in vitro and in vivo.

    PubMed

    Kim, Junghyun; Kim, Ohn Soon; Kim, Chan-Sik; Sohn, Eunjin; Jo, Kyuhyung; Kim, Jin Sook

    2012-02-29

    The formation of advanced glycation end products (AGEs) has been considered to be a potential causative factor of injury to lens epithelial cells (LECs). Damage of LECs is believed to contribute to cataract formation. The purpose of this study was to investigate the cytotoxic effect of AGEs on LECs both in vitro and in vivo. We examined the accumulation of argpyrimidine, a methylglyoxal-derived AGE, and the expression of apoptosis-related molecules including nuclear factor- kappaB (NF-κB), Bax, and Bcl-2 in the human LEC line HLE-B3 and in cataractous lenses of Zucker diabetic fatty (ZDF) rats, an animal model of type 2 diabetes. In cataractous lenses from twenty-oneweek- old ZDF rats, LEC apoptosis was markedly increased, and the accumulation of argpyrimidine as well as subsequent activation of NF-κB in LECs were significantly enhanced. The ratio of Bax to Bcl-2 protein levels was also increased. In addition, the accumulation of argpyrimidine triggered apoptosis in methylglyoxal- treated HLE-B3 cells. However, the presence of pyridoxamine (an AGEs inhibitor) and pyrrolidine dithiocarbamate (a NF-κB inhibitor) prevented apoptosis in HLE-B3 cells through the inhibition of argpyrimidine formation and the blockage of NF-κB nuclear translocalization, respectively. These results suggest that the cellular accumulation of argpyrimidine in LECs is NF-κB-dependent and pro-apoptotic.

  19. Synergistic infection of two viruses MCMV and SCMV increases the accumulations of both MCMV and MCMV-derived siRNAs in maize

    PubMed Central

    Xia, Zihao; Zhao, Zhenxing; Chen, Ling; Li, Mingjun; Zhou, Tao; Deng, Congliang; Zhou, Qi; Fan, Zaifeng

    2016-01-01

    The co-infection of Maize chlorotic mottle virus (MCMV) and Sugarcane mosaic virus (SCMV) can cause maize lethal necrosis. However, the mechanism underlying the synergistic interaction between these two viruses remains elusive. In this study, we found that the co-infection of MCMV and SCMV increased the accumulation of MCMV. Moreover, the profiles of virus-derived siRNAs (vsiRNAs) from MCMV and SCMV in single- and co-infected maize plants were obtained by high-throughput sequencing. Our data showed that synergistic infection of MCMV and SCMV increased remarkably the accumulation of vsiRNAs from MCMV, which were mainly 22 and 21 nucleotides in length. The single-nucleotide resolution maps of vsiRNAs revealed that vsiRNAs were almost continuously but heterogeneously distributed throughout MCMV and SCMV genomic RNAs, respectively. Moreover, we predicted and annotated dozens of host transcript genes targeted by vsiRNAs. Our results also showed that maize DCLs and several AGOs RNAs were differentially accumulated in maize plants with different treatments (mock, single or double inoculations), which were associated with the accumulation of vsiRNAs. Our findings suggested possible roles of vsiRNAs in the synergistic interaction of MCMV and SCMV in maize plants. PMID:26864602

  20. A 9000 year perspective on carbon accumulation rates under changing hydro-climate and vegetation conditions in a mountain peatland, northern Carpathians, Romania

    NASA Astrophysics Data System (ADS)

    Feurdean, Angelica; Panait, Andrei; Gałka, Mariusz; Diaconu, Andrei; Hutchinson, Simon; Mulch, Andreas; Tantau, Ioan; Hickler, Thomas

    2016-04-01

    Peatlands, in particular ombrogenous bogs, which entirely depend on water from precipitation, are sensitive to changes in the balance between precipitation and evapotranspiration; and therefore highly suitable for hydro-climatological reconstruction. Peatlands also represent a large carbon pool in the terrestrial biosphere. However, little is known about the C sequestration processes in mountain peatlands under various competing drivers of change (climate, vegetation, fire). We applied a multi-proxy approach (bulk density, loss on ignition, total organic carbon, testate amoebae, δ13C in Sphagnum, plant macrofossils, pollen and charcoal) to a peat sequence from a mountain ombrogenous bog (Tǎul Muced) to explore how changes in hydro-climate conditions, peat plant composition and fire have affected long-term physical peat properties and the rate of carbon accumulation over the last 9000 years. Carbon accumulation at this site ranged from 7 to 105 g C cm2 yr1 (mean 23 ± 14 g C cm2 yr_1). We found that high moisture availability (P-E) as inferred from testate amoebae and δ13C values in Sphagnum increased the carbon sink capacity of peatland. The strength of the relationship between the rate of carbon accumulation and climate appears particularly evident over the last millennium when high C accumulation rates correlated with the warm and wet conditions of the Medieval Climate Anomaly and lower C accumulation rates with the dry conditions of the Little Ice Age. We also found a significant positive correlation between the rate of C accumulation and changes in vegetation; rates were lowest (17 g C cm2 yr_1), during periods of mixed Sphagnum (primarily S. magellanicum and S. angustifolium) and vascular plant (Cyperaceae, Eriophorum vaginatum) growth and increased (31 g C cm2 yr_1) during the accumulation of Sphagnum peat, regardless the dominant Sphagnum species. We did not find indication of peatland fire during the investigated interval. Our study represents one of the

  1. Seasonal variation of meteorological variables and recent surface ablation / accumulation rates on Davies Dome and Whisky Glacier, James Ross Island, Antarctica

    NASA Astrophysics Data System (ADS)

    Láska, K.; Nývlt, D.; Engel, Z.; Budík, L.

    2012-04-01

    In this study, surface mass balance data of two glaciers on James Ross Island, Antarctica, and its spatial and temporal variations are evaluated using snow ablation stakes, ground-penetrating radar, and dGPS measurements. The investigated glaciers are located on the Ulu Peninsula, northern part of James Ross Island. Davies Dome is an ice dome, which originates on the surface of a flat volcanic mesa at elevations >400 m a.s.l. and terminates with a single 700 m wide outlet in the Whisky Bay. Davies Dome has an area of ~6.5 km2 and lies in the altitude range of 0-514 m a.s.l. Whisky Glacier is a cold-based land-terminating valley glacier surrounded by an extensive moraine ridges made of debris-covered ice. The glacier has an area of ~2.4 km2 and lies in the altitude range of 215-520 m a.s.l. Within several summer austral summers, extensive field programme were carried out on both glaciers including the operation of two automatic weather stations, field mapping and mass balance measurements. Each station was equipped with albedometer CM7B (Kipp-Zonen, Netherlands), air temperature and humidity sensor EMS33 (EMS, Czech Republic), propeller anemometer 05103 (Young, USA), and snow depth sensors (Judd, USA). In the period 2009-2011, high seasonal and interdiurnal variability of incoming solar radiation and near-surface air temperature was found as a result of changes in the circulation patterns and synoptic-scale weather systems moving in the Circumpolar Trough. High ablation and accumulation rates were recorded mainly in the spring and summer seasons (October-February), while negligible changes were found in winter (May-September). The effects of positive degree-day temperatures on the surface ablation rates were examined using a linear regression model. In this approach, near-surface air temperature maps on the glacier surfaces were derived from digital elevation model according to actual temperature lapse rates. Mass balance investigations started in 2006 on Davies

  2. Complex sediment deposition history on a wide continental shelf: Implications for the calculation of accumulation rates on the Great Barrier Reef

    NASA Astrophysics Data System (ADS)

    Lewis, Stephen E.; Olley, Jon; Furuichi, Takahisa; Sharma, Ashneel; Burton, Joanne

    2014-05-01

    Understanding the key processes controlling the delivery, deposition and fate of sediments on continental shelves is critical to appreciate the evolution of coasts and estuaries and to interpret geological sequences. This study presents radiocarbon and Optically Stimulated Luminescence (OSL) ages of sediment cores collected from key locations offshore from the Burdekin River, Australia, the largest single source of sediment delivered to the Great Barrier Reef (GBR) lagoon. The ages show variable sediment accumulation rates at the different locations that coincide with the Holocene avulsion history of the Burdekin River. Our data show that most fine sediment (<63 μm) delivered from the Burdekin River is retained within 50 km of the mouth, a finding that contrasts previous studies which postulated that fine sediments are advected northwards via longshore drift processes. The pairing of radiocarbon and OSL ages provides insights on resuspension regimes operating on the inner shelf of the GBR. It was thought that turbidity on inshore GBR coral reefs and seagrass meadows has increased as a result of increased erosion in the adjacent catchment from agricultural development. Our data show that the age of the sediments in Cleveland Bay (derived from the radiocarbon ages from shell and organic material) can be several thousand years older than when the sediment was last deposited (OSL ages). However, the increased turbidity could conceivably be caused from ‘new biologically-produced sediment’ (i.e. particulate organic matter) as a result of increased nutrient export to the GBR. We suggest that the composition of sediment in resuspension events before and after the wet season be analysed to examine whether newly delivered organic-rich sediment can affect coral reefs and seagrass meadows.

  3. Roles of rhizosphere and root-derived organic acids in Cd accumulation by two hot pepper cultivars.

    PubMed

    Xin, Junliang; Huang, Baifei; Dai, Hongwen; Zhou, Wenjing; Yi, Yumei; Peng, Lijing

    2015-04-01

    Cultivars of hot pepper (Capsicum annuum L.) have different abilities to accumulate Cd in their fruits. Previously, we suggested that low-Cd cultivars take up more Cd, but can better prevent the Cd translocation from roots to aerial parts. However, the mechanisms involved in those processes are still unclear. In this study, we explored the roles of rhizosphere soil Cd fractions and root secretions of low molecular weight organic acids in the uptake, translocation, and accumulation of Cd in a low-Cd and high-Cd cultivar. The results showed that there was no significant difference in exchangeable Cd between rhizosphere soils of the two cultivars, which might be related to their similar root's Cd uptake ability. The total content of low molecular weight organic acids released from roots of the low-Cd cultivar was almost equal to that released from roots of the high-Cd cultivar at the same Cd level; however, the composition of low molecular weight organic acids were determined by cultivars and Cd exposure levels. In the higher Cd (10 μM) treatment, the roots of the low-Cd cultivar excreted significantly less tartaric acid and more oxalic and acetic acids than those of the high-Cd cultivar. Additionally, there was no difference in the concentration of citric or succinic acid between the two cultivars. These results indicate that some kinds of low molecular weight organic acids efflux from hot pepper roots played an important role in the difference of Cd accumulation between low- and high-Cd cultivars.

  4. Lunar meteoritic gardening rate derived from in situ LADEE/LDEX measurements

    NASA Astrophysics Data System (ADS)

    Szalay, Jamey R.; Horányi, Mihály

    2016-05-01

    The Lunar Atmosphere and Dust Environment Explorer (LADEE) orbited the Moon for approximately 6 months, taking data with the Lunar Dust Experiment (LDEX). LDEX was uniquely equipped to characterize the current rate of lunar impact gardening as it measured the very particles taking part in this process. By deriving an average lunar dust density distribution, we calculate the rate at which exospheric dust rains back down onto the lunar surface. Near the equatorial plane, we find that approximately 40 μm/Myr of lunar regolith, with a cumulative size distribution index of 2.7, is redistributed due to meteoritic bombardment, a process which occurs predominantly on the lunar apex hemisphere.

  5. Derivation of the chemical-equilibrium rate coefficient using scattering theory

    NASA Technical Reports Server (NTRS)

    Mickens, R. E.

    1977-01-01

    Scattering theory is applied to derive the equilibrium rate coefficient for a general homogeneous chemical reaction involving ideal gases. The reaction rate is expressed in terms of the product of a number of normalized momentum distribution functions, the product of the number of molecules with a given internal energy state, and the spin-averaged T-matrix elements. An expression for momentum distribution at equilibrium for an arbitrary molecule is presented, and the number of molecules with a given internal-energy state is represented by an expression which includes the partition function.

  6. Thirty years of satellite-derived lava discharge rates at Etna: Implications for steady volumetric output

    NASA Astrophysics Data System (ADS)

    Harris, Andrew; Steffke, Andrea; Calvari, Sonia; Spampinato, Letizia

    2011-08-01

    We present a 30 year long data set of satellite-derived time-averaged lava discharge rates (TADR) for Mount Etna volcano (Sicily, Italy), spanning 1980-2010 and comprising 1792 measurements during 23 eruptions. We use this to classify eruptions on the basis of magnitude and intensity, as well as the shape of the TADR time series which characterizes each effusive event. We find that while 1983-1993 was characterized by less frequent but longer-duration effusive eruptions at lower TADRs, 2000-2010 was characterized by more frequent eruptions of shorter duration and higher TADRs. However, roughly the same lava volume was erupted during both of these 11 year long periods, so that the volumetric output was linear over the entire 30 year period, increasing at a rate of 0.8 m3 s-1 between 1980 and 2010. The cumulative volume record can be extended back in time using data available in the literature. This allows us to assess Etna's output history over 5 centuries and to place the current trend in historical context. We find that output has been stable at this rate since 1971. At this time, the output rate changed from a low discharge rate phase, which had characterized the period 1759 to 1970, to a high discharge rate phase. This new phase had the same output rate as the high discharge rate phase that characterized the period 1610-1669. The 1610-1669 phase ended with the most voluminous eruption of historic times.

  7. Predominant anthropogenic sources and rates of atmospheric mercury accumulation in southern Ontario recorded by peat cores from three bogs: comparison with natural "background" values (past 8000 years).

    PubMed

    Givelet, Nicolas; Roos-Barraclough, Fiona; Shotyk, William

    2003-12-01

    Peat cores from three bogs in southern Ontario provide a complete, quantitative record of net rates of atmospheric Hg accumulation since pre-industrial times. For comparison with modern values, a peat core extending back 8000 years was used to quantify the natural variations in Hg fluxes for this region, and their dependence on climatic change and land use history. The net mercury accumulation rates were separated into "natural" and "excess" components by comparing the Hg/Br ratios of modern samples with the long-term, pre-anthropogenic average Hg/Br. The average background mercury accumulation rate during the pre-anthropogenic period (from 5700 years BC to 1470 AD) was 1.4 +/- 1.0 microg m(-2) per year (n = 197). The beginning of Hg contamination from anthropogenic sources dates from AD 1475 at the Luther Bog, corresponding to biomass burning for agricultural activities by Native North Americans. During the late 17th and 18th centuries, deposition of anthropogenic Hg was at least equal to that of Hg from natural sources. Anthropogenic inputs of Hg to the bogs have dominated continuously since the beginning of the 19th century. The maximum Hg accumulation rates decrease in the order Sifton Bog, in the City of London, Ontario (141 microg Hg m(-2) per year), Luther Bog in an agricultural region (89 microg Hg m(-2) per year), and Spruce Bog which is in a comparatively remote, forested region (54 microg Hg m(-2) per year). Accurate age dating of recent peat samples using the bomb pulse curve of 14C shows that the maximum rate of atmospheric Hg accumulation occurred during AD 1956 and 1959 at all sites. In these (modern) samples, the Hg concentration profiles resemble those of Pb, an element which is known to be immobile in peat bogs. The correlation between these two metals, together with sulfur, suggests that the predominant anthropogenic source of Hg (and Pb) was coal burning. While Hg accumulation rates have gone into strong decline since the late 1950's, Hg

  8. Variations in the accumulation, localization and rate of metabolization of selenium in mature Zea mays plants supplied with selenite or selenate.

    PubMed

    Longchamp, Mélanie; Castrec-Rouelle, Maryse; Biron, Philippe; Bariac, Thierry

    2015-09-01

    Quantification of selenium bioavailability from foods is a key challenge following the discovery of the antioxidant role of this micronutrient in human health. This study presents the uptake, accumulation and rate of metabolization in mature Zea mays plants grown in hydroponic solution supplemented with selenate or selenite. Selenium content was lower in plants supplemented with selenate and accumulated mainly in the leaves compared with selenite-treated plants where the selenium was retained in the roots. Selenite-treated grains accumulated more selenium. Selenate was metabolized less than selenite in whole plants, but in grains selenium was present exclusively as organic selenium compounds. For humans, the bioavailability of organic selenium was evaluated at 90% compared with only 50% for inorganic forms. Our results show that the potential for selenium bioavailability is increased with selenite treatment.

  9. Three hen strains fed photoisomerized trans,trans CLA-rich soy oil exhibit different yolk accumulation rates and source-specific isomer deposition.

    PubMed

    Shinn, Sara E; Gilley, Alex D; Proctor, Andrew; Anthony, Nicholas B

    2015-04-01

    Most CLA chicken feeding trials used cis,trans (c,t) and trans,cis (t,c) CLA isomers to produce CLA-rich eggs, while reports of trans,trans (t,t) CLA enrichment in egg yolks are limited. The CLA yolk fatty acid profile changes and the 10-12 days of feeding needed for maximum CLA are well documented, but there is no information describing CLA accumulation during initial feed administration. In addition, no information on CLA accumulation rates in different hen strains is available. The aim of this study was to determine a mathematical model that described yolk CLA accumulation and depletion in three hen strains by using t,t CLA-rich soybean oil produced by photoisomerization. Diets of 30-week Leghorns, broilers, and jungle fowl were supplemented with 15% CLA-rich soy oil for 16 days, and eggs were collected for 32 days. Yolk fatty acid profiles were measured by GC-FID. CLA accumulation and depletion was modeled by both quadratic and piecewise regression analysis. A strong quadratic model was proposed, but it was not as effective as piecewise regression in describing CLA accumulation and depletion. Broiler hen eggs contained the greatest concentration of CLA at 3.2 mol/100 g egg yolk, then jungle fowl at 2.9 mol CLA, and Leghorns at 2.3 mol CLA. The t,t CLA isomer levels remained at 55% of total yolk CLA during CLA feeding. However, t-10,c-12 (t,c) CLA concentration increased slightly during CLA accumulation and was significantly greater than c-9,t-11 CLA. Jungle fowl had the smallest increase in yolk saturated fat with CLA yolk accumulation.

  10. Germline mutation rates and the long-term phenotypic effects of mutation accumulation in wild-type laboratory mice and mutator mice

    PubMed Central

    Uchimura, Arikuni; Higuchi, Mayumi; Minakuchi, Yohei; Ohno, Mizuki; Toyoda, Atsushi; Fujiyama, Asao; Miura, Ikuo; Wakana, Shigeharu; Nishino, Jo; Yagi, Takeshi

    2015-01-01

    The germline mutation rate is an important parameter that affects the amount of genetic variation and the rate of evolution. However, neither the rate of germline mutations in laboratory mice nor the biological significance of the mutation rate in mammalian populations is clear. Here we studied genome-wide mutation rates and the long-term effects of mutation accumulation on phenotype in more than 20 generations of wild-type C57BL/6 mice and mutator mice, which have high DNA replication error rates. We estimated the base-substitution mutation rate to be 5.4 × 10−9 (95% confidence interval = 4.6 × 10−9–6.5 × 10−9) per nucleotide per generation in C57BL/6 laboratory mice, about half the rate reported in humans. The mutation rate in mutator mice was 17 times that in wild-type mice. Abnormal phenotypes were 4.1-fold more frequent in the mutator lines than in the wild-type lines. After several generations, the mutator mice reproduced at substantially lower rates than the controls, exhibiting low pregnancy rates, lower survival rates, and smaller litter sizes, and many of the breeding lines died out. These results provide fundamental information about mouse genetics and reveal the impact of germline mutation rates on phenotypes in a mammalian population. PMID:26129709

  11. Germline mutation rates and the long-term phenotypic effects of mutation accumulation in wild-type laboratory mice and mutator mice.

    PubMed

    Uchimura, Arikuni; Higuchi, Mayumi; Minakuchi, Yohei; Ohno, Mizuki; Toyoda, Atsushi; Fujiyama, Asao; Miura, Ikuo; Wakana, Shigeharu; Nishino, Jo; Yagi, Takeshi

    2015-08-01

    The germline mutation rate is an important parameter that affects the amount of genetic variation and the rate of evolution. However, neither the rate of germline mutations in laboratory mice nor the biological significance of the mutation rate in mammalian populations is clear. Here we studied genome-wide mutation rates and the long-term effects of mutation accumulation on phenotype in more than 20 generations of wild-type C57BL/6 mice and mutator mice, which have high DNA replication error rates. We estimated the base-substitution mutation rate to be 5.4 × 10(-9) (95% confidence interval = 4.6 × 10(-9)-6.5 × 10(-9)) per nucleotide per generation in C57BL/6 laboratory mice, about half the rate reported in humans. The mutation rate in mutator mice was 17 times that in wild-type mice. Abnormal phenotypes were 4.1-fold more frequent in the mutator lines than in the wild-type lines. After several generations, the mutator mice reproduced at substantially lower rates than the controls, exhibiting low pregnancy rates, lower survival rates, and smaller litter sizes, and many of the breeding lines died out. These results provide fundamental information about mouse genetics and reveal the impact of germline mutation rates on phenotypes in a mammalian population.

  12. Significant relationships among frost tolerance and net photosynthetic rate, water use efficiency and dehydrin accumulation in cold-treated winter oilseed rapes.

    PubMed

    Urban, Milan Oldřich; Klíma, Miroslav; Vítámvás, Pavel; Vašek, Jakub; Hilgert-Delgado, Alois Albert; Kučera, Vratislav

    2013-12-15

    Five winter oilseed rape cultivars (Benefit, Californium, Cortes, Ladoga, Navajo) were subjected to 30 days of cold treatment (4 °C) to examine the effect of cold on acquired frost tolerance (FT), dehydrin (DHN) content, and photosynthesis-related parameters. The main aim of this study was to determine whether there are relationships between FT (expressed as LT50 values) and the other parameters measured in the cultivars. While the cultivar Benefit accumulated two types of DHNs (D45 and D35), the other cultivars accumulated three additional DHNs (D97, D47, and D37). The similar-sized DHNs (D45 and D47) were the most abundant; the others exhibited significantly lower accumulations. The highest correlations were detected between LT50 and DHN accumulation (r=-0.815), intrinsic water use efficiency (WUEi; r=-0.643), net photosynthetic rate (r=-0.628), stomatal conductance (r=0.511), and intracellular/intercellular CO2 concentration (r=0.505). Those cultivars that exhibited higher Pn rate in cold (and further a significant increase in WUEi) had higher levels of DHNs and also higher FT. No significant correlation was observed between LT50 and E, PRI, or NDVI. Overall, we have shown the selected physiological parameters to be able to distinguish different FT cultivars of winter oilseed rape.

  13. Stress Field Variation after the 2001 Skyros Earthquake, Greece, Derived from Seismicity Rate Changes

    NASA Astrophysics Data System (ADS)

    Leptokaropoulos, K.; Papadimitriou, E.; Orlecka-Sikora, B.; Karakostas, V.

    2012-04-01

    The spatial variation of the stress field (ΔCFF) after the 2001 strong (Mw=6.4) Skyros earthquake in North Aegean Sea, Greece, is investigated in association with the changes of earthquake production rates. A detailed slip model is considered in which the causative fault is consisted of several sub-faults with different coseismic slip onto each one of them. First the spatial distribution of aftershock productivity is compared with the static stress changes due to the coseismic slip. Calculations of ΔCFF are performed at different depths inside the seismogenic layer, defined from the vertical distribution of the aftershocks. Seismicity rates of the smaller magnitude events with M≥Mc for different time increments before and after the main shock are then derived from the application of a Probability Density Function (PDF). These rates are computed by spatially smoothing the seismicity and for this purpose a normal grid of rectangular cells is superimposed onto the area and the PDF determines seismicity rate values at the center of each cell. The differences between the earthquake occurrence rates before and after the main shock are compared and used as input data in a stress inversion algorithm based upon the Rate/State dependent friction concept in order to provide an independent estimation of stress changes. This model incorporates the physical properties of the fault zones (characteristic relaxation time, fault constitutive parameters, effective friction coefficient) with a probabilistic estimation of the spatial distribution of seismicity rates, derived from the application of the PDF. The stress patterns derived from the previously mentioned approaches are compared and the quantitative correlation between the respective results is accomplished by the evaluation of Pearson linear correlation coefficient and its confidence intervals to quantify their significance. Different assumptions and combinations of the physical and statistical parameters are tested for

  14. The effect of interest rate derivative transactions on debt savings for not-for-profit health systems.

    PubMed

    Venkataramani, Prakash; Johnson, Tricia; O'Neil, Patricia; Poindexter, Victoria; Rooney, Jeffrey

    2006-01-01

    The utilization of interest rate derivative instruments in US for-profit companies has grown exponentially since the early 1980s. International Swaps and Derivatives Association, Inc. (ISDA), reported that the amount of outstanding standard swaps grew by 25 percent during the first six months of 2003. The growth rate of all interest rate derivatives, which includes single-currency interest rate swaps, cross-currency interest rate swaps, and interest rate options, grew by 24 percent during the same period. The total outstanding amount of interest rate derivatives now totals $123.9 trillion compared to $99.9 trillion at the end of 2002 (Dodd, 2003). This explosion in usage is a testament to the efficacy and flexibility of the instruments and the increased appreciation by financial managers of the importance of financial risk management in a volatile interest rate environment.

  15. Linearization of digital derived rate algorithm for use in linear stability analysis

    NASA Technical Reports Server (NTRS)

    Graham, R. E.; Porada, T. W.

    1985-01-01

    The digital derived rate (DDR) algorithm is used to calculate the rate of rotation of the Centaur upper-stage rocket. The DDR is highly nonlinear algorithm, and classical linear stability analysis of the spacecraft cannot be performed without linearization. The performance of this rate algorithm is characterized by a gain and phase curve that drop off at the same frequency. This characteristic is desirable for many applications. A linearization technique for the DDR algorithm is investigated. The linearization method is described. Examples of the results of the linearization technique are illustrated, and the effects of linearization are described. A linear digital filter may be used as a substitute for performing classical linear stability analyses, while the DDR itself may be used in time response analysis.

  16. Accurate derivation of heart rate variability signal for detection of sleep disordered breathing in children.

    PubMed

    Chatlapalli, S; Nazeran, H; Melarkod, V; Krishnam, R; Estrada, E; Pamula, Y; Cabrera, S

    2004-01-01

    The electrocardiogram (ECG) signal is used extensively as a low cost diagnostic tool to provide information concerning the heart's state of health. Accurate determination of the QRS complex, in particular, reliable detection of the R wave peak, is essential in computer based ECG analysis. ECG data from Physionet's Sleep-Apnea database were used to develop, test, and validate a robust heart rate variability (HRV) signal derivation algorithm. The HRV signal was derived from pre-processed ECG signals by developing an enhanced Hilbert transform (EHT) algorithm with built-in missing beat detection capability for reliable QRS detection. The performance of the EHT algorithm was then compared against that of a popular Hilbert transform-based (HT) QRS detection algorithm. Autoregressive (AR) modeling of the HRV power spectrum for both EHT- and HT-derived HRV signals was achieved and different parameters from their power spectra as well as approximate entropy were derived for comparison. Poincare plots were then used as a visualization tool to highlight the detection of the missing beats in the EHT method After validation of the EHT algorithm on ECG data from the Physionet, the algorithm was further tested and validated on a dataset obtained from children undergoing polysomnography for detection of sleep disordered breathing (SDB). Sensitive measures of accurate HRV signals were then derived to be used in detecting and diagnosing sleep disordered breathing in children. All signal processing algorithms were implemented in MATLAB. We present a description of the EHT algorithm and analyze pilot data for eight children undergoing nocturnal polysomnography. The pilot data demonstrated that the EHT method provides an accurate way of deriving the HRV signal and plays an important role in extraction of reliable measures to distinguish between periods of normal and sleep disordered breathing (SDB) in children.

  17. Sediment accumulation rates and high-resolution stratigraphy of recent fluvial suspension deposits in various fluvial settings, Morava River catchment area, Czech Republic

    NASA Astrophysics Data System (ADS)

    Sedláček, Jan; Bábek, Ondřej; Kielar, Ondřej

    2016-02-01

    We present a comprehensive study concerning sedimentary processes in fluvial sediment traps within the Morava River catchment area (Czech Republic) involving three dammed reservoirs, four meanders and oxbow lakes, and several natural floodplain sites. The objective of the study was to determine sediment accumulation rates (SAR), estimate erosion rates, calculating these using a combination of the 137Cs method and historical data. Another purpose of this study was to provide insight into changing erosion and accumulation rates over the last century. Extensive water course modifications were carried out in the Morava River catchment area during the twentieth century, which likely affected sedimentation rates along the river course. Other multiproxy stratigraphic methods (X-ray densitometry, magnetic susceptibility, and visible-light reflectance spectrometry) were applied to obtain additional information about sediment infill. Sediment stratigraphy revealed distinct distal-to-proximal patterns, especially in reservoirs. Granulometrically, silts and sandy silts prevailed in sediments. Oxbow lakes and meanders contained larger amounts of clay and organic matter, which is the main difference between them and reservoirs. Pronounced 137Cs peaks were recorded in all studied cores (maximum 377 Bq·kg- 1), thus indicating Chernobyl fallout from 1986 or older events. Calculated sediment accumulation rates were lowest in distal parts of reservoirs (0.13-0.58 cm/y) and floodplains (0.45-0.88 cm/y), moderately high rates were found in proximal parts of reservoirs and oxbow lakes (2.27-4.4 cm/y), and the highest rates in some oxbow lakes located near the river (6-8 cm/y). The frequency of the inundation still can be high in some natural areas as in the Litovelské Pomoraví protected area, whereas the decreasing frequency of the inundation in other modified parts can contribute to a lower sedimentation rate. The local effects such as difference between SARs in oxbow lakes and

  18. Increasing sediment accumulation rates in La Fonera (Palamós) submarine canyon axis and their relationship with bottom trawling activities

    NASA Astrophysics Data System (ADS)

    Puig, P.; Martín, J.; Masqué, P.; Palanques, A.

    2015-10-01

    Previous studies conducted in La Fonera (Palamós) submarine canyon (NW Mediterranean) found that trawling activities along the canyon flanks cause resuspension and transport of sediments toward the canyon axis. 210Pb chronology supported by 137Cs dating applied to a sediment core collected at 1750 m in 2002 suggested a doubling of the sediment accumulation rate since the 1970s, coincident with the rapid industrialization of the local trawling fleet. The same canyon area has been revisited a decade later, and new data are consistent with a sedimentary regime shift during the 1970s and also suggest that the accumulation rate during the last decade could be greater than expected, approaching ~2.4 cm yr-1 (compared to ~0.25 cm yr-1 pre-1970s). These results support the hypothesis that commercial bottom trawling can substantially affect sediment dynamics and budgets on continental margins, eventually initiating the formation of anthropogenic depocenters in submarine canyon environments.

  19. Comprehensive modeling and investigation of the effect of iron on the growth rate and lipid accumulation of Chlorella vulgaris cultured in batch photobioreactors.

    PubMed

    Concas, Alessandro; Steriti, Alberto; Pisu, Massimo; Cao, Giacomo

    2014-02-01

    Recent works have shown that specific strains of microalgae are capable to simultaneously increase their growth rate and lipid content when cultured under suitable concentrations of iron. While these results are promising in view of the exploitation of microalgae for producing biofuels, to the best of our knowledge, no mathematical model capable to describe the effect of iron on lipid accumulation in microalgae, has been so far proposed. A comprehensive mathematical model describing the effect of iron on chlorophyll synthesis, nitrogen assimilation, growth rate and lipid accumulation in a freshwater strain of Chlorella vulgaris is then proposed in this work. Model results are successfully compared with experimental data which confirm the positive effect of growing iron concentrations on lipid productivity of C. vulgaris. Thus, the proposed model might represent a useful tool to optimize iron-based strategies to improve the lipid productivity of microalgal cultures.

  20. Preferential accumulation of sex and Bs chromosomes in biarmed karyotypes by meiotic drive and rates of chromosomal changes in fishes.

    PubMed

    Molina, Wagner F; Martinez, Pablo A; Bertollo, Luiz A C; Bidau, Claudio J

    2014-12-01

    Mechanisms of accumulation based on typical centromeric drive or of chromosomes carrying pericentric inversions are adjusted to the general karyotype differentiation in the principal Actinopterygii orders. Here, we show that meiotic drive in fish is also supported by preferential establishment of sex chromosome systems and B chromosomes in orders with predominantly bi-brachial chromosomes. The mosaic of trends acting at an infra-familiar level in fish could be explained as the interaction of the directional process of meiotic drive as background, modulated on a smaller scale by adaptive factors or specific karyotypic properties of each group, as proposed for the orthoselection model.

  1. How Integrated Management Strategies Promote Protein Quality of Cotton Embryos: High Levels of Soil Available N, N Assimilation and Protein Accumulation Rate

    PubMed Central

    Yang, HongKun; Meng, YaLi; Chen, BingLin; Zhang, XingYue; Wang, YouHua; Zhao, WenQing; Zhou, ZhiGuo

    2016-01-01

    Cottonseed is widely used as a source of ruminant feed and for industrial purposes. Therefore, there is a tremendous need to improve the nutritional value of cotton embryos. In this study, a conventional management (CM) and two integrated cotton management strategies (IMS1, IMS2) were performed at two soil fertility levels to study the relationships among soil N, N assimilation, embryonic protein accumulation and protein quality. The levels of proteins, essential amino acids, and semi-essential amino acids, especially those of glutamate, lysine, and methionine, were higher in IMS1 and IMS2 embryos than in CM embryos. These changes were significantly positively correlated with the soil-available N content, glutamine synthetase activity and peak value of protein accumulation rate and were negatively correlated with the free amino acid level. These results illustrated that integrated management strategies, especially the rates and timing of N application, raise the level of soil available N, which is beneficial for N assimilation in developing cotton embryos. The protein content was limited by the rate of protein accumulation rather than by the free amino acid content. The combination of target yield fertilization, a growth-driven N application schedule, a high plant density and the seedling raising with bio-organic fertilizer can substantially improve protein quality in cotton embryos, especially at a soil with low soil organic matter and total nitrogen. PMID:27532007

  2. How Integrated Management Strategies Promote Protein Quality of Cotton Embryos: High Levels of Soil Available N, N Assimilation and Protein Accumulation Rate.

    PubMed

    Yang, HongKun; Meng, YaLi; Chen, BingLin; Zhang, XingYue; Wang, YouHua; Zhao, WenQing; Zhou, ZhiGuo

    2016-01-01

    Cottonseed is widely used as a source of ruminant feed and for industrial purposes. Therefore, there is a tremendous need to improve the nutritional value of cotton embryos. In this study, a conventional management (CM) and two integrated cotton management strategies (IMS1, IMS2) were performed at two soil fertility levels to study the relationships among soil N, N assimilation, embryonic protein accumulation and protein quality. The levels of proteins, essential amino acids, and semi-essential amino acids, especially those of glutamate, lysine, and methionine, were higher in IMS1 and IMS2 embryos than in CM embryos. These changes were significantly positively correlated with the soil-available N content, glutamine synthetase activity and peak value of protein accumulation rate and were negatively correlated with the free amino acid level. These results illustrated that integrated management strategies, especially the rates and timing of N application, raise the level of soil available N, which is beneficial for N assimilation in developing cotton embryos. The protein content was limited by the rate of protein accumulation rather than by the free amino acid content. The combination of target yield fertilization, a growth-driven N application schedule, a high plant density and the seedling raising with bio-organic fertilizer can substantially improve protein quality in cotton embryos, especially at a soil with low soil organic matter and total nitrogen.

  3. Combined effects of water flow and copper concentration on the feeding behavior, growth rate, and accumulation of copper in tissue of the infaunal polychaete Polydora cornuta.

    PubMed

    Colvin, Marienne A; Hentschel, Brian T; Deheyn, Dimitri D

    2016-12-01

    We performed an experiment in a laboratory flume to test the effects of water flow speed and the concentration of aqueaous copper on the feeding behavior, growth rate, and accumulation of copper in the tissues of juvenile polychaetes Polydora cornuta. The experiment included two flow speeds (6 or 15 cm/s) and two concentrations of added copper (0 or 85 μg/L). Worms grew significantly faster in the faster flow and in the lower copper concentration. In the slower flow, the total time worms spent feeding decreased significantly as copper concentration increased, but copper did not significantly affect the time worms spent feeding in the faster flow. Across all treatments, there was a significant, positive relationship between the time individuals spent feeding and their relative growth rate. Worms were observed suspension feeding significantly more often in the faster flow and deposit feeding significantly more often in the slower flow, but copper concentration did not affect the proportion of time spent in either feeding mode. The addition of 85 μg/L copper significantly increased copper accumulation in P. cornuta tissue, but the accumulation did not differ significantly due to flow speed. There was a significant interaction between copper and flow; the magnitude of the difference in copper accumulation between the 0 and 85 μg/L treatments was greater in the faster flow than in the slower flow. In slow flows that favor deposit feeding, worms grow slowly and accumulate less copper in their tissue than in faster flows that favor suspension feeding and faster growth.

  4. Malfunction in Mitochondrial β-Oxidation Contributes to Lipid Accumulation in Hepatocyte-Like Cells Derived from Citrin Deficiency-Induced Pluripotent Stem Cells.

    PubMed

    Kim, Yeji; Choi, Jung-Yun; Lee, Sang-Hee; Lee, Beom-Hee; Yoo, Han-Wook; Han, Yong-Mahn

    2016-04-15

    Citrin deficiency (CD) is a recessive genetic disorder caused by mutations in the citrin gene SLC25A13. CD causes various symptoms related to nutrient metabolism such as urea cycle failure, abnormal amino acid levels, and fatty liver. To understand the pathophysiology of CD, the molecular phenotypes were investigated using induced pluripotent stem cells derived from fibroblasts of CD patient (CD-iPSCs). In this study, we demonstrate that aberrant mitochondrial β-oxidation may lead to fatty liver in CD patients. CD-iPSCs normally differentiated into hepatocytes, similar to wild-type iPSCs (WT-iPSCs). However, hepatocytes derived from CD-iPSCs (CD-HLCs) did not exhibit ureogenesis. Cellular triglyceride and lipid granule levels were significantly increased in CD-HLCs compared with WT-HLCs. Peroxisome proliferator-activated receptor-α (PPAR-α) and its target genes which are involved in mitochondrial β-oxidation were downregulated in CD-HLCs, and treatment with a PPAR-α agonist partially reduced the lipid accumulation in CD-HLCs. In addition, the mitochondria in CD-HLCs exhibited abnormal morphologies. Based on these observations, we conclude that the lipid accumulation in CD-HLCs results from dysfunctional mitochondrial β-oxidation and abnormal mitochondrial structure.

  5. Tectonic control on 10Be-derived erosion rates in the Garhwal Himalaya, India

    NASA Astrophysics Data System (ADS)

    Scherler, Dirk; Bookhagen, Bodo; Strecker, Manfred R.

    2014-02-01

    Erosion in the Himalaya is responsible for one of the greatest mass redistributions on Earth and has fueled models of feedback loops between climate and tectonics. Although the general trends of erosion across the Himalaya are reasonably well known, the relative importance of factors controlling erosion is less well constrained. Here we present 25 10Be-derived catchment-averaged erosion rates from the Yamuna catchment in the Garhwal Himalaya, northern India. Tributary erosion rates range between ~0.1 and 0.5 mm yr-1 in the Lesser Himalaya and ~1 and 2 mm yr-1 in the High Himalaya, despite uniform hillslope angles. The erosion-rate data correlate with catchment-averaged values of 5 km radius relief, channel steepness indices, and specific stream power but to varying degrees of nonlinearity. Similar nonlinear relationships and coefficients of determination suggest that topographic steepness is the major control on the spatial variability of erosion and that twofold to threefold differences in annual runoff are of minor importance in this area. Instead, the spatial distribution of erosion in the study area is consistent with a tectonic model in which the rock uplift pattern is largely controlled by the shortening rate and the geometry of the Main Himalayan Thrust fault (MHT). Our data support a shallow dip of the MHT underneath the Lesser Himalaya, followed by a midcrustal ramp underneath the High Himalaya, as indicated by geophysical data. Finally, analysis of sample results from larger main stem rivers indicates significant variability of 10Be-derived erosion rates, possibly related to nonproportional sediment supply from different tributaries and incomplete mixing in main stem channels.

  6. Shallow Sediment Trace Metal Concentrations and Short-Term Accumulation Rates in the Neponset River Estuary, Massachusetts, USA

    NASA Astrophysics Data System (ADS)

    Spencer, J. R.; Zhu, J.; Olsen, C. R.

    2010-12-01

    The Neponset River estuary is a small estuary that drains into the Boston Harbor on the east coast of the United States. It is also a highly urbanized estuary and has a long history of urban development over 450 years. In July 2006, six sediment cores were collected in the Neponset River estuary to examine particle dynamics and sediment accumulation via radionuclide (Beryllium-7) dating, and to determine sediment metal concentrations (As, Cu, Pb, and Zn) via ED-XRF measurements. Measured sediment Be-7 profiles indicate various sedimentation environments, where sediment accumulation, resuspension or redeposition is likely to occur. High metal concentrations were often corresponding to high Be-7 inventories in sediment cores. Possible sources of trace metal contaminants in the water column include: storm water run-off, Combined Sewer Overflows (CSOs), a well-documented industrial pollution event that occurred upstream in the early to mid twentieth century, and the resuspension of sediment. Existing and future data will provide baseline information for quantifying the effects of the proposed and pending environmental restoration project, which includes the removal of the Baker Dam. The combined pre- and post-Dam removal data may then be used in cost-benefit analyses for other similar estuarine restoration projects.

  7. Effects of an anti-oxidative ACAT inhibitor on apoptosis/necrosis and cholesterol accumulation under oxidative stress in THP-1 cell-derived foam cells.

    PubMed

    Miike, Tomohiro; Shirahase, Hiroaki; Jino, Hiroshi; Kunishiro, Kazuyoshi; Kanda, Mamoru; Kurahashi, Kazuyoshi

    2008-01-02

    THP-1 cell-derived foam cells were exposed to oxidative stress through combined treatment with acetylated LDL (acLDL) and copper ions (Cu2+). The foam cells showed caspase-dependent apoptotic changes on exposure to oxidative stress for 6 h, and necrotic changes with the leakage of LDH after 24 h. KY-455, an anti-oxidative ACAT inhibitor, and ascorbic acid (VC) but not YM-750, an ACAT inhibitor, prevented apoptotic and necrotic changes. These preventive effects of KY-455 and VC were accompanied by the inhibition of lipid peroxidation in culture medium containing acLDL and Cu2+, suggesting the involvement of oxidized acLDL in apoptosis and necrosis. Foam cells accumulated esterified cholesterol (EC) for 24 h in the presence of acLDL without Cu2+, which was suppressed by KY-455 and YM-750. Foam cells showed necrotic changes and died in the presence of acLDL and Cu2+. KY-455 but not YM-750 prevented cell death and reduced the amount of EC accumulated. The foam cells treated with VC further accumulated EC without necrotic changes for 24 h even in the presence of acLDL and Cu2+. YM-750 as well as KY-455 inhibited lipid accumulation when co-incubated with VC in foam cells exposed to oxidative stress. It is concluded that an anti-oxidative ACAT inhibitor or the combination of an antioxidant and an ACAT inhibitor protects foam cells from oxidative stress and effectively reduces cholesterol levels, which would be a promising approach in anti-atherosclerotic therapy.

  8. Mycorrhizal-mediated lower proline accumulation in Poncirus trifoliata under water deficit derives from the integration of inhibition of proline synthesis with increase of proline degradation.

    PubMed

    Zou, Ying-Ning; Wu, Qiang-Sheng; Huang, Yong-Ming; Ni, Qiu-Dan; He, Xin-Hua

    2013-01-01

    Proline accumulation was often correlated with drought tolerance of plants infected by arbuscular mycorrhizal fungi (AMF), whereas lower proline in some AM plants including citrus was also found under drought stress and the relevant mechanisms have not been fully elaborated. In this study proline accumulation and activity of key enzymes relative to proline biosynthesis (▵(1)-pyrroline-5-carboxylate synthetase, P5CS; ornithine-δ-aminotransferase, OAT) and degradation (proline dehydrogenase, ProDH) were determined in trifoliate orange (Poncirus trifoliata, a widely used citrus rootstock) inoculated with or without Funneliformis mosseae and under well-watered (WW) or water deficit (WD). AMF colonization significantly increased plant height, stem diameter, leaf number, root volume, biomass production of both leaves and roots and leaf relative water content, irrespectively of water status. Water deficit induced more tissue proline accumulation, in company with an increase of P5CS activity, but a decrease of OAT and ProDH activity, no matter whether under AM or no-AM. Compared with no-AM treatment, AM treatment resulted in lower proline concentration and content in leaf, root, and total plant under both WW and WD. The AMF colonization significantly decreased the activity of both P5CS and OAT in leaf, root, and total plant under WW and WD, except for an insignificant difference of root OAT under WD. The AMF inoculation also generally increased tissue ProDH activity under WW and WD. Plant proline content significantly positively correlated with plant P5CS activity, negatively with plant ProDH activity, but not with plant OAT activity. These results suggest that AM plants may suffer less from WD, thereby inducing lower proline accumulation, which derives from the integration of an inhibition of proline synthesis with an enhancement of proline degradation.

  9. Mycorrhizal-Mediated Lower Proline Accumulation in Poncirus trifoliata under Water Deficit Derives from the Integration of Inhibition of Proline Synthesis with Increase of Proline Degradation

    PubMed Central

    Zou, Ying-Ning; Wu, Qiang-Sheng; Huang, Yong-Ming; Ni, Qiu-Dan; He, Xin-Hua

    2013-01-01

    Proline accumulation was often correlated with drought tolerance of plants infected by arbuscular mycorrhizal fungi (AMF), whereas lower proline in some AM plants including citrus was also found under drought stress and the relevant mechanisms have not been fully elaborated. In this study proline accumulation and activity of key enzymes relative to proline biosynthesis (▵1-pyrroline-5-carboxylate synthetase, P5CS; ornithine-δ-aminotransferase, OAT) and degradation (proline dehydrogenase, ProDH) were determined in trifoliate orange (Poncirus trifoliata, a widely used citrus rootstock) inoculated with or without Funneliformis mosseae and under well-watered (WW) or water deficit (WD). AMF colonization significantly increased plant height, stem diameter, leaf number, root volume, biomass production of both leaves and roots and leaf relative water content, irrespectively of water status. Water deficit induced more tissue proline accumulation, in company with an increase of P5CS activity, but a decrease of OAT and ProDH activity, no matter whether under AM or no-AM. Compared with no-AM treatment, AM treatment resulted in lower proline concentration and content in leaf, root, and total plant under both WW and WD. The AMF colonization significantly decreased the activity of both P5CS and OAT in leaf, root, and total plant under WW and WD, except for an insignificant difference of root OAT under WD. The AMF inoculation also generally increased tissue ProDH activity under WW and WD. Plant proline content significantly positively correlated with plant P5CS activity, negatively with plant ProDH activity, but not with plant OAT activity. These results suggest that AM plants may suffer less from WD, thereby inducing lower proline accumulation, which derives from the integration of an inhibition of proline synthesis with an enhancement of proline degradation. PMID:24260421

  10. Patient-derived xenograft (PDX) tumors increase growth rate with time.

    PubMed

    Pearson, Alexander T; Finkel, Kelsey A; Warner, Kristy A; Nör, Felipe; Tice, David; Martins, Manoela D; Jackson, Trachette L; Nör, Jacques E

    2016-02-16

    Patient-derived xenograft (PDX) models are frequently used for translational cancer research, and are assumed to behave consistently as the tumor ages. However, growth rate constancy as a function of time is unclear. Notably, variable PDX growth rates over time might have implications for the interpretation of translational studies. We characterized four PDX models through several in vivo passages from primary human head and neck squamous cell carcinoma and salivary gland adenoid cystic carcinoma. We developed a mathematical approach to merge growth data from different passages into a single measure of relative tumor volume normalized to study initiation size. We analyzed log-relative tumor volume increase with linear mixed effect models. Two oral pathologists analyzed the PDX tissues to determine if histopathological feature changes occurred over in vivo passages. Tumor growth rate increased over time. This was determined by repeated measures linear regression statistical analysis in four different PDX models. A quadratic statistical model for the temporal effect predicted the log-relative tumor volume significantly better than a linear time effect model. We found a significant correlation between passage number and histopathological features of higher tumor grade. Our mathematical treatment of PDX data allows statistical analysis of tumor growth data over long periods of time, including over multiple passages. Non-linear tumor growth in our regression models revealed the exponential growth rate increased over time. The dynamic tumor growth rates correlated with quantifiable histopathological changes that related to passage number in multiple types of cancer.

  11. Patient-derived xenograft (PDX) tumors increase growth rate with time

    PubMed Central

    Pearson, Alexander T.; Finkel, Kelsey A.; Warner, Kristy A.; Nör, Felipe; Tice, David; Martins, Manoela D.; Jackson, Trachette L.; Nör, Jacques E.

    2016-01-01

    Patient-derived xenograft (PDX) models are frequently used for translational cancer research, and are assumed to behave consistently as the tumor ages. However, growth rate constancy as a function of time is unclear. Notably, variable PDX growth rates over time might have implications for the interpretation of translational studies. We characterized four PDX models through several in vivo passages from primary human head and neck squamous cell carcinoma and salivary gland adenoid cystic carcinoma. We developed a mathematical approach to merge growth data from different passages into a single measure of relative tumor volume normalized to study initiation size. We analyzed log-relative tumor volume increase with linear mixed effect models. Two oral pathologists analyzed the PDX tissues to determine if histopathological feature changes occurred over in vivo passages. Tumor growth rate increased over time. This was determined by repeated measures linear regression statistical analysis in four different PDX models. A quadratic statistical model for the temporal effect predicted the log-relative tumor volume significantly better than a linear time effect model. We found a significant correlation between passage number and histopathological features of higher tumor grade. Our mathematical treatment of PDX data allows statistical analysis of tumor growth data over long periods of time, including over multiple passages. Non-linear tumor growth in our regression models revealed the exponential growth rate increased over time. The dynamic tumor growth rates correlated with quantifiable histopathological changes that related to passage number in multiple types of cancer. PMID:26783960

  12. Accumulation Rates in the Dry Snow Zone of the Greenland Ice Sheet Inferred from L-band InSAR Data

    NASA Astrophysics Data System (ADS)

    Chen, A. C.; Zebker, H. A.

    2012-12-01

    The Greenland ice sheet contains about 2.9 million km3 of ice and would raise global sea levels by about 7.1 m if it melted completely. Two unusually large iceberg calving events at Petermann Glacier in the past several years, along with the unusually large extent of ice sheet melt this summer point to the relevance of understanding the mass balance of the Greenland ice sheet. In this study, we use data from the PALSAR instrument aboard the ALOS satellite to form L-band (23-centimeter carrier wavelength) InSAR images of the dry snow zone of the Greenland ice sheet. We form geocoded differential interferograms, using the ice sheet elevation model produced by Howat et.al. [1]. By applying phase and radiometric calibration, we can examine interferograms formed between any pair of transmit and receive polarization channels. In co-polarized interferograms, the InSAR correlation ranges from about 0.35 at the summit (38.7 deg W, 73.0 deg N) where accumulation is about 20 cm w.e./yr to about 0.70 at the north-eastern part of the dry snow zone (35.1 deg W, 77.1 deg N), where accumulation is about 11.7 cm w.e./yr. Cross-polarized interferograms show similar geographic variation with overall lower correlation. We compare our InSAR data with in-situ measurements published by Bales et.al. [2]. We examine the applicability of dense-medium radiative transfer electromagnetic scattering models for estimating accumulation rates from L-band InSAR data. The large number and broad coverage of ALOS scenes acquired between 2007 and 2009 with good InSAR coherence at 46-day repeat times and 21.5 degree incidence angles gives us the opportunity to examine the empirical relationship between in-situ accumulation rate observations and the polarimetric InSAR correlation and radar brightness at this particular imaging geometry. This helps us quantify the accuracy of accumulation rates estimated from InSAR data. In some regions, 46-day interferograms acquired in the winters of several consecutive

  13. Present-day CGPS-derived Crustal Strain Rate Field of the Saint Lawrence River Valley

    NASA Astrophysics Data System (ADS)

    Goudarzi, M. A.; Cocard, M.; Santerre, R.

    2015-12-01

    The Saint Lawrence River valley (SLRV) is one of the most seismically active areas in eastern Canada. Along the SLRV and the Ottawa valley, earthquakes are concentrated on three distinct zones of western Quebec along the Ottawa River, Charlevoix, and Lower Saint Lawrence. The entire area is also subject to the glacial isostatic adjustment (GIA). We studied the earth's surface deformation of the area using the velocity field of 51 continuous GPS (CGPS) stations and the least-squares collocation method. While the intraplate horizontal velocities showed a coherent horizontal motion towards southeast with the typical magnitude of ~1.3 mm/yr for stations along the SLRV, the interpolated vertical velocities demonstrated a coherent uplift with the average rate of 3.1 mm/yr. We estimated strain rate tensors including the effect of vertical velocity. A NNW-SSE shortening with a typical rate of ~3.6-8.1 nstrain/yr was observed over Lower Saint Lawrence. In Charlevoix, an extension with a typical rate of ~3.0-7.1 nstrain/yr was oriented in ENE-WSW parallel to the SLRV. In western Quebec, the deformation has a shear straining mechanism with a typical shortening rate of ~1.0-5.1 nstrain/yr and extension rate of ~1.6-4.1 nstrain/yr. The extension over the northern model is consistent with the prediction of the GIA models. The range of the estimated strain rates of the area (~1.0-8.1 nstrain/yr) is between typical values of rigid blocks (< 0.1 nstrain/yr) and active tectonic regions (> 100 μstrain/yr). A strong correlation was observed between epicenters of earthquakes and areas with the highest rate of shear strain. We found a good agreement between the orientations of the principal axes of strain rate tensors and the maximum horizontal compressional stress σH from World Stress Map 2008 for both strike-slip and thrust faulting regimes especially those derived from focal mechanisms. This shows our CGPS intraplate velocities are representative of the current crustal deformation

  14. Replacement of the glucose phosphotransferase transport system by galactose permease reduces acetate accumulation and improves process performance of Escherichia coli for recombinant protein production without impairment of growth rate.

    PubMed

    De Anda, Ramón; Lara, Alvaro R; Hernández, Vanessa; Hernández-Montalvo, Verónica; Gosset, Guillermo; Bolívar, Francisco; Ramírez, Octavio T

    2006-05-01

    Acetate accumulation under aerobic conditions is a common problem in Escherichia coli cultures, as it causes a reduction in both growth rate and recombinant protein productivity. In this study, the effect of replacing the glucose phosphotransferase transport system (PTS) with an alternate glucose transport activity on growth kinetics, acetate accumulation and production of two model recombinant proteins, was determined. Strain VH32 is a W3110 derivative with an inactive PTS. The promoter region of the chromosomal galactose permease gene galP of VH32 was replaced by the strong trc promoter. The resulting strain, VH32GalP+ acquired the capacity to utilize glucose as a carbon source. Strains W3110 and VH32GalP+ were transformed for the production of recombinant TrpLE-proinsulin accumulated as inclusion bodies (W3110-PI and VH32GalP+-PI) and for production of soluble intracellular green fluorescent protein (W3110-pV21 and VH32GalP+-pV21). W3110-pV21 and VH32GalP+-pV21 were grown in batch cultures. Maximum recombinant protein concentration, as determined from fluorescence, was almost four-fold higher in VH32GalP+-pV21, relative to W3110-pV21. Maximum acetate concentration reached 2.8 g/L for W3110-pV21 cultures, whereas a maximum of 0.39 g/L accumulated in VH32GalP+-pV21. W3110-PI and VH32GalP+-PI were grown in batch and fed-batch cultures. Compared to W3110-PI, the engineered strain maintained similar production and growth rate capabilities while reducing acetate accumulation. Specific glucose consumption rate was lower and product yield on glucose was higher in VH32GalP+-PI fed-batch cultures. Altogether, strains with the engineered glucose uptake system showed improved process performance parameters for recombinant protein production over the wild-type strain.

  15. Effect of altering the root-zone temperature on growth, translocation, carbon exchange rate, and leaf starch accumulation in the tomato.

    PubMed

    Hurewitz, J; Janes, H W

    1983-09-01

    Tomato seedlings (Lycopersicon esculentum Mill. cv Vendor) were grown hydroponically with their root systems maintained at a constant temperature for a 2-week period commencing with the appearance of the first true leaf. Based on fresh and dry weight and leaf area, the optimal root-zone temperature for seedling growth was 30 degrees C. The carbon exchange rate of the leaves was also found to increase with rising root-zone temperature up to 30 degrees C. However, a more complex relationship seems to exist between root-zone temperature and the accumulation of (14)C-labeled assimilates in the roots; inasmuch as there is no enhancement in this accumulation at the most growth promoting root-zone temperatures (22-30 degrees C).

  16. Influence of satellite-derived photolysis rates and NOx emissions on Texas ozone modeling

    NASA Astrophysics Data System (ADS)

    Tang, W.; Cohan, D. S.; Pour-Biazar, A.; Lamsal, L. N.; White, A.; Xiao, X.; Zhou, W.; Henderson, B. H.; Lash, B.

    2014-09-01

    Uncertain photolysis rates and emission inventory impair the accuracy of state-level ozone (O3) regulatory modeling. Past studies have separately used satellite-observed clouds to correct the model-predicted photolysis rates, or satellite-constrained top-down NOx emissions to identify and reduce uncertainties in bottom-up NOx emissions. However, the joint application of multiple satellite-derived model inputs to improve O3 State Implementation Plan (SIP) modeling has rarely been explored. In this study, Geostationary Operational Environmental Satellite (GOES) observations of clouds are applied to derive the photolysis rates, replacing those used in Texas SIP modeling. This changes modeled O3 concentrations by up to 80 ppb and improves O3 simulations by reducing modeled normalized mean bias (NMB) and normalized mean error (NME) by up to 0.1. A sector-based discrete Kalman filter (DKF) inversion approach is incorporated with the Comprehensive Air Quality Model with extensions (CAMx)-Decoupled Direct Method (DDM) model to adjust Texas NOx emissions using a high resolution Ozone Monitoring Instrument (OMI) NO2 product. The discrepancy between OMI and CAMx NO2 vertical column densities (VCD) is further reduced by increasing modeled NOx lifetime and adding an artificial amount of NO2 in the upper troposphere. The sector-based DKF inversion tends to scale down area and non-road NOx emissions by 50%, leading to a 2-5 ppb decrease in ground 8 h O3 predictions. Model performance in simulating ground NO2 and O3 are improved using inverted NOx emissions, with 0.25 and 0.04 reductions in NMBs and 0.13 and 0.04 reductions in NMEs, respectively. Using both GOES-derived photolysis rates and OMI-constrained NOx emissions together reduces modeled NMB and NME by 0.05 and increases the model correlation with ground measurement in O3 simulations and makes O3 more sensitive to NOx emissions in the O3 non-attainment areas.

  17. Geologic ages and accumulation rates of basalt-flow groups and sedimentary interbeds in selected wells at the Idaho National Engineering Laboratory, Idaho

    SciTech Connect

    Anderson, S.R.; Liszewski, M.J.; Cecil, L.D.

    1997-01-01

    Geologic ages and accumulation rates, estimated from regressions, were used to evaluate measured ages and interpreted stratigraphic and structural relations of basalt and sediment in the unsaturated zone and the Snake River Plain aquifer at the Idaho National Engineering Laboratory (INEL) in eastern Idaho. Geologic ages and accumulation rates were estimated from standard linear regressions of 21 mean potassium-argon (K-Ar) ages, selected mean paleomagnetic ages, and cumulative depths of a composite stratigraphic section composed of complete intervals of basalt and sediment that were deposited in areas of past maximum subsidence. Accumulation rates also were estimated from regressions of stratigraphic intervals in three wells in and adjacent to an area of interpreted uplift at and near the Idaho Chemical Processing Plant (ICPP) and the Test Reactor Area (TRA) to allow a comparison of rates in areas of past uplift and subsidence. Estimated geologic ages range from about 200 thousand to 1.8 million years before present and are reasonable approximations for the interval of basalt and sediment above the effective base of the aquifer, based on reported uncertainties of corresponding measured ages. Estimated ages between 200 and 800 thousand years are within the range of reported uncertainties for all 15 K-Ar ages used in regressions and two out of three -argon ({sup 40}Ar/{sup 39}Ar) ages of duplicate argon samples. Two sets of estimated ages between 800 thousand and 1.8 million years are within the range of reported uncertainties for all seven K-Ar ages used in regressions, which include one shared age of about 800 thousand years. Two sets of ages were estimated for this interval because K-Ar ages make up two populations that agree with previous and revised ages of three paleomagnetic subchrons. The youngest set of ages is consistent with a K-Ar age from the effective base of the aquifer that agrees with previous ages of the Olduvai Normal-Polarity Subchron.

  18. Accumulation and altered localization of telomere-associated protein TRF2 in immortally transformed and tumor-derived human breast cells

    SciTech Connect

    Nijjar, Tarlochan; Bassett, Ekaterina; Garbe, James; Takenaka, Yasuhiro; Stampfer, Martha R.; Gilley, David; Yaswen, Paul

    2004-12-23

    We have used cultured human mammary epithelial cells (HMEC) and breast tumor-derived lines to gain information on defects that occur during breast cancer progression. HMEC immortalized by a variety of agents (the chemical carcinogen benzo(a)pyrene, oncogenes c-myc and ZNF217, and/or dominant negative p53 genetic suppressor element GSE22) displayed marked up regulation (10-15 fold) of the telomere binding protein, TRF2. Up-regulation of TRF2 protein was apparently due to differences in post-transcriptional regulation, as mRNA levels remained comparable in finite life span and immortal HMEC. TRF2 protein was not up-regulated by the oncogenic agents alone in the absence of immortalization, nor by expression of exogenously introduced hTERT genes. We found TRF2 levels to be at least 2-fold higher than in control cells in 11/15 breast tumor cell lines, suggesting that elevated TRF2 levels are a frequent occurrence during the transformation of breast tumor cells in vivo. The dispersed distribution of TRF2 throughout the nuclei in some immortalized and tumor-derived cells indicated that not all the TRF2 was associated with telomeres in these cells. The process responsible for accumulation of TRF2 in immortalized HMEC and breast tumor-derived cell lines may promote tumorigenesis by contributing to the cells ability to maintain an indefinite life span.

  19. Leaf senescence in rice due to magnesium deficiency mediated defect in transpiration rate before sugar accumulation and chlorosis.

    PubMed

    Kobayashi, Natsuko I; Saito, Takayuki; Iwata, Naoko; Ohmae, Yoshimi; Iwata, Ren; Tanoi, Keitaro; Nakanishi, Tomoko M

    2013-08-01

    Magnesium (Mg) is an essential macronutrient supporting various functions, including photosynthesis. However, the specific physiological responses to Mg deficiency remain elusive. In this study, 2-week-old rice seedlings (Oryza sativa. cv. Nipponbare) with three expanded leaves (L2-L4) were transferred to Mg-free nutrient solution for 8 days. In the absence of Mg, on day 8, L5 and L6 were completely developed, while L7 just emerged. We also studied several mineral deficiencies to identify specific responses to Mg deficiency. Each leaf was analyzed in terms of chlorophyll, starch, anthocyanin and carbohydrate metabolites, and only absence of Mg was found to cause irreversible senescence of L5. Resupply of Mg at various time points confirmed that the borderline of L5 death was between days 6 and 7 of Mg deficiency treatment. Decrease in chlorophyll concentration and starch accumulation occurred simultaneously in L5 and L6 blades on day 8. However, nutrient transport drastically decreased in L5 as early as day 6. These data suggest that the predominant response to Mg deficiency is a defect in transpiration flow. Furthermore, changes in myo-inositol and citrate concentrations were detected only in L5 when transpiration decreased, suggesting that they may constitute new biological markers of Mg deficiency.

  20. Estimating sediment accumulation rates in Manila Bay, a marine pollution hot spot in the Seas of East Asia.

    PubMed

    Sta Maria, E J; Siringan, F P; Bulos, A dM; Sombrito, E Z

    2009-01-01

    The GEF/UNDP/IMO/PEMSEA project identifies Manila Bay as among the marine pollution hot spots in the Seas of East Asia. (210)Pb dating of its sediment can provide a historical perspective of its pollution loading. However, the validity of (210)Pb dating in a complex dynamic coastal system of Manila Bay may come into question. Land-based sediment input can be high and physical and biological processes can possibly disturb the sediment layers. In this report, the (210)Pb profiles of sediment cores from different parts of the bay are presented. The linear sedimentation rates are shown to be higher in the recent past and are also variable across the bay. The largest change in sedimentation rate, coincided with the occurrence of a volcanic eruption in 1991 and is shown by applying a variant of the CIC model in sedimentation rate calculations. The data suggest that (210)Pb dating can be useful in estimating relative magnitudes of sedimentation rates, even in a complex dynamic coastal system like Manila Bay.

  1. Effects of feeding and organism loading rate on PCB accumulation by Lumbriculus variegatus in sediment bioaccumulation testing

    EPA Science Inventory

    Sediment bioaccumulation test methods published by USEPA and ASTM in 2000 specify that the Lumbriculus variegatus, a freshwater oligochaete, should not be fed during the 28-day exposure and recommends an organism loading rate of total organic carbon in sediment to organism dry we...

  2. Human Adipose-Derived Stem Cells Expanded Under Ambient Oxygen Concentration Accumulate Oxidative DNA Lesions and Experience Procarcinogenic DNA Replication Stress.

    PubMed

    Bétous, Rémy; Renoud, Marie-Laure; Hoede, Claire; Gonzalez, Ignacio; Jones, Natalie; Longy, Michel; Sensebé, Luc; Cazaux, Christophe; Hoffmann, Jean-Sébastien

    2017-01-01

    Adipose-derived stem cells (ADSCs) have led to growing interest in cell-based therapy because they can be easily harvested from an abundant tissue. ADSCs must be expanded in vitro before transplantation. This essential step causes concerns about the safety of adult stem cells in terms of potential transformation. Tumorigenesis is driven in its earliest step by DNA replication stress, which is characterized by the accumulation of stalled DNA replication forks and activation of the DNA damage response. Thus, to evaluate the safety of ADSCs during ex vivo expansion, we monitored DNA replication under atmospheric (21%) or physiologic (1%) oxygen concentration. Here, by combining immunofluorescence and DNA combing, we show that ADSCs cultured under 21% oxygen accumulate endogenous oxidative DNA lesions, which interfere with DNA replication by increasing fork stalling events, thereby leading to incomplete DNA replication and fork collapse. Moreover, we found by RNA sequencing (RNA-seq) that culture of ADSCs under atmospheric oxygen concentration leads to misexpression of cell cycle and DNA replication genes, which could contribute to DNA replication stress. Finally, analysis of acquired small nucleotide polymorphism shows that expansion of ADSCs under 21% oxygen induces a mutational bias toward deleterious transversions. Overall, our results suggest that expanding ADSCs at a low oxygen concentration could reduce the risk for DNA replication stress-associated transformation, as occurs in neoplastic tissues. Stem Cells Translational Medicine 2017;6:68-76.

  3. Human Adipose-Derived Stem Cells Expanded Under Ambient Oxygen Concentration Accumulate Oxidative DNA Lesions and Experience Procarcinogenic DNA Replication Stress.

    PubMed

    Bétous, Rémy; Renoud, Marie-Laure; Hoede, Claire; Gonzalez, Ignacio; Jones, Natalie; Longy, Michel; Sensebé, Luc; Cazaux, Christophe; Hoffmann, Jean-Sébastien

    2016-08-24

    : Adipose-derived stem cells (ADSCs) have led to growing interest in cell-based therapy because they can be easily harvested from an abundant tissue. ADSCs must be expanded in vitro before transplantation. This essential step causes concerns about the safety of adult stem cells in terms of potential transformation. Tumorigenesis is driven in its earliest step by DNA replication stress, which is characterized by the accumulation of stalled DNA replication forks and activation of the DNA damage response. Thus, to evaluate the safety of ADSCs during ex vivo expansion, we monitored DNA replication under atmospheric (21%) or physiologic (1%) oxygen concentration. Here, by combining immunofluorescence and DNA combing, we show that ADSCs cultured under 21% oxygen accumulate endogenous oxidative DNA lesions, which interfere with DNA replication by increasing fork stalling events, thereby leading to incomplete DNA replication and fork collapse. Moreover, we found by RNA sequencing (RNA-seq) that culture of ADSCs under atmospheric oxygen concentration leads to misexpression of cell cycle and DNA replication genes, which could contribute to DNA replication stress. Finally, analysis of acquired small nucleotide polymorphism shows that expansion of ADSCs under 21% oxygen induces a mutational bias toward deleterious transversions. Overall, our results suggest that expanding ADSCs at a low oxygen concentration could reduce the risk for DNA replication stress-associated transformation, as occurs in neoplastic tissues.

  4. HLA-G promotes myeloid-derived suppressor cell accumulation and suppressive activity during human pregnancy through engagement of the receptor ILT4.

    PubMed

    Köstlin, Natascha; Ostermeir, Anna-Lena; Spring, Bärbel; Schwarz, Julian; Marmé, Alexander; Walter, Christina B; Poets, Christian F; Gille, Christian

    2017-02-01

    Establishing and maintaining maternal-fetal tolerance is essential for a successful pregnancy; failure of immunological adaptation to pregnancy leads to severe complications such as abortion or preterm delivery. Myeloid-derived suppressor cells (MDSCs) are innate immune cells that suppress T-cell responses, expand during pregnancy and thus may play a role in tolerance induction. Human leucocyte antigen G (HLA-G) is a major histocompatibility complex (MHC) I molecule with immune-modulatory properties, which is expressed during pregnancy. Here, we investigated the impact of HLA-G on MDSCs accumulation and activation in pregnant women. We demonstrate that granulocytic MDSCs (GR-MDSCs) express receptors for HLA-G, namely immunoglobulin-like transcript (ILT) 2 and 4, and that ILT4-expression by GR-MDSCs is regulated during pregnancy. Stimulation with soluble HLA-G (sHLA-G) increased suppressive activity of GR-MDSCs, induced MDSCs from peripheral blood mononuclear cells (PBMCs) and led to phosphorylation of the signal transducer and activator of transcription 3 (STAT3) and induction of indoleamine-2,3-dioxygenase (IDO) in myeloid cells. Effects of sHLA-G on MDSC accumulation were mediated through ILT4. These results suggest an interaction between MDSCs and HLA-G in humans as a potential mechanism for maintaining maternal-fetal tolerance. Modulating MDSC function during pregnancy via HLA-G might provide new opportunities for a therapeutic manipulation of immunological pregnancy complications.

  5. Lithosphere thickness and mantle viscosity inverted from GPS-derived deformation rates in Fennoscandia

    NASA Astrophysics Data System (ADS)

    Zhao, S.; Lambeck, K.; Lidberg, M.

    2012-07-01

    Crustal deformation in Fennoscandia is associated with the glacial isostatic adjustment (GIA) process that is caused by ongoing stress release of the mantle after removal of the Late Pleistocene ice sheet by ˜10 cal ka BP. With an earth model of defined structure and rheology and an ice-sheet model of known melting history, the GIA process can be simulated by geophysical models, and the surface deformation rates can be calculated and used to compare with global positioning system (GPS) observations. Therefore, the crustal deformation rates observed by GPS in Fennoscandia provide constraints on the geophysical models. On the basis of two ice sheet models (ANU-ICE and ICE-5G) reconstructed independently by the Australian National University (ANU) and University of Toronto, we use the GPS-derived deformation rates to invert for lithosphere thickness and mantle viscosity in Fennoscandia. The results show that only a three-layer earth model can be resolved from current GPS data, providing robust estimates of effective lithosphere thickness, upper and lower mantle viscosity. The earth models estimated from inversion of GPS data with two different ice sheet models define a narrow range of parameter space: the lithosphere thickness between 93 and 110 km, upper mantle viscosity between 3.4 and 5.0 × 1020 Pa s, and lower mantle viscosity between 7 × 1021 and 13 × 1021 Pa s. The estimates are consistent with those inverted from relative sea-level indicators.

  6. Sedimentation rates in eastern North America reveal strong links between regional climate, depositional environments, and sediment accumulation

    NASA Astrophysics Data System (ADS)

    Goring, S. J.; McLachlan, J. S.; Jackson, S. T.; Blaauw, M.; Christen, J.; Marlon, J.; Blois, J.; Williams, J. W.

    2011-12-01

    PalEON is a multidisciplinary project that combines paleo and modern ecological data with state-of-the-art statistical and modelling tools to examine the interactions between climate, fire and vegetation during the past two millennia in the northeastern United States. A fundamental challenge for PalEON (and paleo research more broadly) is to improve age modelling to yield more accurate sediment-core chronologies. To address this challenge, we assessed sedimentation rates and their controls for 218 lakes and mires in the northeastern U.S. Sedimentation rates (yr/cm) were calculated from age-depth models, which were obtained from the Neotoma database (www.neotomadb.org) and other contributed pollen records. The age models were recalibrated to IntCal09 and augmented in some cases using biostratigraphic markers (Picea decline, 16 kcal BP - 10.5 kcal BP; Quercus rise, 12 - 9.1 kcal BP; and Alnus decline, 11.5 - 10.6 kcal BP) as described in Blois et al. (2011). Relationships between sedimentation rates and sediment age, site longitude, and depositional environment (lacustrine or mire) are significant but weak. There are clear and significant links between variations in the NGRIP record of δ18O and sedimentation in mires across the PalEON region, but no links to lacustrine sedimentation rates. This result indicates that super-regional climatic control of primary productivity, and thus autochthonic sediment deposition, dominates in mires while deposition in lacustrine basins may be driven primarily by local and regional factors including watershed size, surficial materials,and regional vegetation. The shape of the gamma probability functions that best describe sedimentation rate distributions are calculated and presented here for use as priors in Bayesian age modelling applications such as BACON (Blaauw and Christen, in press). Future applications of this research are also discussed.

  7. Archean high δ18O Mg-diorite: crustal-derived melt hybridized with enriched mafic accumulated rocks

    NASA Astrophysics Data System (ADS)

    Wang, Dan; Guo, Jing-Hui

    2016-04-01

    The genesis of Mg-diorite or sanukitoids has significances to understand the crustal growth and tectonic style in Archean. The chemical compositions of minerals and rocks, whole-rock Sm-Nd isotope, zircon SIMS U-Pb ages and Hf-O isotopes of Zhulagou (ZLG) Mg-diorite and their mafic enclaves (Yinshan Block, North China Craton) were studied to place constraints on their sources and genesis, and therefore provide information about dynamic processes. The ~2520 Ma ZLG diorites have intermediate SiO2 (59.4-65.5 wt.%), high Mg# (49-52), Cr (90.4-438 ppm), Ni (15.0-95.9 ppm), Sr (436-882 ppm) and Ba (237-1206 ppm) contents with fractionated rare earth elements (REE, LaN/YbN = 9.1-40.5) and depleted high field-strength element (HFSE, e.g. Nb, Ta and Ti). These geochemical signatures are similar to those Archean high-Mg diorites and sanukitoids. However, they are sodic with low K2O/Na2O (0.14-0.49) ratios, exhibiting an affinity with Archean trondhjemite-tonalite-granodiorite (TTG). Abundant coeval amphibole-bearing mafic enclaves (~2525 Ma) are enclosed within the ZLG diorites. They display low SiO2 (46.5-50.3 wt.%) contents but high concentrations of MgO (9.0-14.5 wt.%), Cr (647-1946 ppm) and Ni (197-280 ppm). They are enriched in K2O (0.64-3.43 wt.%) and large ion lithophile element (LILE), depleted in Nb, Ta and Ti. Combined with their concave REE patterns and prominent negative Eu anomaly, we suggest that they are cumulates of the melt which probably derived from subduction-related Archean metasomatized mantle source. Mineral trace element modelling results, similar ɛNd(t) (+0.6 to +2.3) and δ18O(Zrc) values (~8.6-9.0 ‰) of the diorites and mafic enclaves, strongly reflect that they had experienced intense interaction and hybridization. Evolved whole-rock Nd isotopes (TDM = 2.80-2.70 Ga), variable zircon ɛHf (t) (-1.6 to +6.0) and high δ18O (~9.0 ‰) values of the diorites indicate that they most likely originated from melting of an older continental crust (≥ 2

  8. Urban rivers as conveyors of hydrocarbons to sediments of estuarine areas: source characterization, flow rates and mass accumulation.

    PubMed

    Mauad, Cristiane R; Wagener, Angela de L R; Massone, Carlos G; Aniceto, Mayara da S; Lazzari, Letícia; Carreira, Renato S; Farias, Cássia de O

    2015-02-15

    Aliphatic (n-C12-n-C40, unresolved complex mixture, resolved peaks) and aromatic hydrocarbons (46 PAH) were investigated in suspended particulate matter (SPM) sampled over eleven months in six of the major rivers and two channels of the Guanabara Bay Basin. PAH flow rates of the most contaminated rivers, the contribution to the PAH sediment load of the receiving bay, and the main sources of hydrocarbons were determined. PAH (38) ranged from 28 ng L(-1) to 11,514 ng L(-1). Hydrocarbon typology and statistical evaluation demonstrated contribution of distinct sources in different regions and allowed quantification of these contributions. Total flow rate for the five major rivers amounts to 3 t year(-1) and responds for 30% of the total PAH annual input into the northern area of the Guanabara Bay. For the first time PAH mass deposited in the bay sediments has been estimated and shall serve as base for decision making and source abatement.

  9. Auroral energy deposition rate, characteristic electron energy, and ionospheric parameters derived from Dynamics Explorer 1 images

    NASA Technical Reports Server (NTRS)

    Rees, M. H.; Lummerzheim, D.; Roble, R. G.; Winningham, J. D.; Craven, J. D.

    1988-01-01

    Auroral images obtained by the Spin Scan Auroral Imager (SAI) aboard the DE-1 satellite were used to derive auroral energy deposition rate, characteristic electron energy, and ionospheric parameters. The principles involved in the imaging technique and the physical mechanisms that underlie the relationship between the spectral images and the geophysical parameters are discussed together with the methodology for implementing such analyses. It is shown that images obtained with the SAI provide global parameters at 12-min temporal resolution; the spatial resolution is limited by the field of view of a pixel. The analysis of the 12-min images presented yielded a representation of ionospheric parameters that was better than can be obtained using empirical models based on local measurements averaged over long periods of time.

  10. Hypoxic culture conditions induce increased metabolic rate and collagen gene expression in ACL-derived cells.

    PubMed

    Kowalski, Tomasz J; Leong, Natalie L; Dar, Ayelet; Wu, Ling; Kabir, Nima; Khan, Adam Z; Eliasberg, Claire D; Pedron, Andrew; Karayan, Ashant; Lee, Siyoung; Di Pauli von Treuheim, Theodor; Jiacheng, Jin; Wu, Ben M; Evseenko, Denis; McAllister, David R; Petrigliano, Frank A

    2016-06-01

    There has been substantial effort directed toward the application of bone marrow and adipose-derived mesenchymal stromal cells (MSCs) in the regeneration of musculoskeletal tissue. Recently, resident tissue-specific stem cells have been described in a variety of mesenchymal structures including ligament, tendon, muscle, cartilage, and bone. In the current study, we systematically characterize three novel anterior cruciate ligament (ACL)-derived cell populations with the potential for ligament regeneration: ligament-forming fibroblasts (LFF: CD146(neg) , CD34(neg) CD44(pos) , CD31(neg) , CD45(neg) ), ligament perivascular cells (LPC: CD146(pos) CD34(neg) CD44(pos) , CD31(neg) , CD45(neg) ) and ligament interstitial cells (LIC: CD34(pos) CD146(neg) , CD44(pos) , CD31(neg) , CD45(neg) )-and describe their proliferative and differentiation potential, collagen gene expression and metabolism in both normoxic and hypoxic environments, and their trophic potential in vitro. All three groups of cells (LIC, LPC, and LFF) isolated from adult human ACL exhibited progenitor cell characteristics with regard to proliferation and differentiation potential in vitro. Culture in low oxygen tension enhanced the collagen I and III gene expression in LICs (by 2.8- and 3.3-fold, respectively) and LFFs (by 3- and 3.5-fold, respectively) and increased oxygen consumption rate and extracellular acidification rate in LICs (by 4- and 3.5-fold, respectively), LFFs (by 5.5- and 3-fold, respectively), LPCs (by 10- and 4.5-fold, respectively) as compared to normal oxygen concentration. In summary, this study demonstrates for the first time the presence of three novel progenitor cell populations in the adult ACL that demonstrate robust proliferative and matrix synthetic capacity; these cells may play a role in local ligament regeneration, and consequently represent a potential cell source for ligament engineering applications. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc

  11. Acoustic sensor versus electrocardiographically derived respiratory rate in unstable trauma patients.

    PubMed

    Yang, Shiming; Menne, Ashley; Hu, Peter; Stansbury, Lynn; Gao, Cheng; Dorsey, Nicolas; Chiu, William; Shackelford, Stacy; Mackenzie, Colin

    2016-06-07

    Respiratory rate (RR) is important in many patient care settings; however, direct observation of RR is cumbersome and often inaccurate, and electrocardiogram-derived RR (RRECG) is unreliable. We asked how data derived from the first 15 min of RR recording after trauma center admission using a novel acoustic sensor (RRa) would compare to RRECG and to end-tidal carbon dioxide-based RR ([Formula: see text]) from intubated patients, the "gold standard" in predicting life-saving interventions in unstable trauma patients. In a convenience sample subset of trauma patients admitted to our Level 1 trauma center, enrolled in the ONPOINT study, and monitored with RRECG, some of whom also had [Formula: see text] data, we collected RRa using an adhesive sensor with an integrated acoustic transducer (Masimo RRa™). Using Bland-Altman analysis of area under the receiver operating characteristic (AUROC) curves, we compared the first 15 min of continuous RRa and RRECG to [Formula: see text] and assessed the performance of these three parameters compared to the Revised Trauma Score (RTS) in predicting blood transfusion 3, 6, and 12 h after admission. Of the 1200 patients enrolled in ONPOINT from December 2011 to May 2013, 1191 had RRECG data recorded in the first 15 min, 358 had acoustic monitoring, and 14 of the latter also had [Formula: see text]. The three groups did not differ demographically or in mechanism of injury. RRa showed less bias (0.8 vs. 6.9) and better agreement than RRECG when compared to [Formula: see text]. At [Formula: see text] 10-29 breaths per minute, RRa was more likely to be the same as [Formula: see text] and assign the same RTS. In predicting transfusion, features derived from RRa and RRECG gave AUROCs 0.59-0.66 but with true positive rate 0.70-0.89. RRa monitoring is a non-invasive option to glean valid RR data to assist clinical decision making and could contribute to prediction models in non-intubated unstable trauma patients.

  12. Pancreatic adenocarcinoma up-regulated factor (PAUF) enhances the accumulation and functional activity of myeloid-derived suppressor cells (MDSCs) in pancreatic cancer.

    PubMed

    Song, Jinhoi; Lee, Jaemin; Kim, Jinsil; Jo, Seongyea; Kim, Yeon Jeong; Baek, Ji Eun; Kwon, Eun-Soo; Lee, Kwang-Pyo; Yang, Siyoung; Kwon, Ki-Sun; Kim, Dong-Uk; Kang, Tae Heung; Park, Yun-Yong; Chang, Suhwan; Cho, Hee Jun; Kim, Song Cheol; Koh, Sang Seok; Kim, Seokho

    2016-08-09

    Pancreatic cancer is characterized by an immunosuppressive tumor microenvironment (TME) with a profound immune infiltrate populated by a significant number of myeloid-derived suppressor cells (MDSCs). MDSCs have been increasingly recognized for their role in immune evasion and cancer progression as well as their potential as a target for immunotherapy. However, not much is known about the mechanisms regulating their behavior and function in the pancreatic TME. Here we report that pancreatic adenocarcinoma up-regulated factor (PAUF), a soluble protein involved in pancreatic tumorigenesis and metastasis, plays a role as an enhancer of tumor-infiltrating MDSC and its functional activity. We show that PAUF enhanced the accumulation of MDSCs in the spleen and tumor tissues of PAUF-overexpressing tumor cell-injected mice. In addition, PAUF was found to enhance the immunosuppressive function of MDSCs via the TLR4-mediated signaling pathway, which was demonstrated by PAUF-induced increased levels of arginase, nitric oxide (NO), and reactive oxygen species (ROS). The role of PAUF in modulating the functional properties of MDSCs was further demonstrated by the use of a PAUF-neutralizing antibody that caused a decreased number of tumor-infiltrating MDSCs and reduced MDSC immunosuppressive activity. The observations made in mice were confirmed in human pancreatic cancer patient-derived MDSCs, supporting the clinical relevance of our findings. Collectively, we conclude that the PAUF is a powerful and multifunctional promoter of tumor growth through increase and functional activation of MDSCs, suggesting therapeutic potential for targeting PAUF in pancreatic cancers.

  13. Escherichia coli-Derived Uracil Increases the Antibacterial Activity and Growth Rate of Lactobacillus plantarum.

    PubMed

    Ha, Eun-Mi

    2016-05-28

    Lactobacillus plantarum (L. plantarum) is a representative probiotic. In particular, L. plantarum is the first commensal bacterium to colonize the intestine of infants. For this reason, the initial settlement of L. plantarum can play an important role in determining an infant's health as well as their eventual health status as an adult. In addition, L. plantarum combats pathogenic infections (such as Escherichia coli (E. coli), one of the early pathogenic colonizers in an unhealthy infant gut) by secreting antimicrobial substances. The aim of this research was to determine how L. plantarum combats E. coli infection and why it is a representative probiotic in the intestine. Consequently, this research observed that E. coli releases uracil. L. plantarum specifically recognizes E. coli-derived uracil, which increases the growth rate and production of antimicrobial substance of L. plantarum. In addition, through the inhibitory activity test, this study postulates that the antimicrobial substance is a protein and can be considered a bacteriocin-like substance. Therefore, this research assumes that L. plantarum exerts its antibacterial ability by recognizing E. coli and increasing its growth rate as a result, and this phenomenon could be one of the reasons for L. plantarum settling in the intestine of infants as a beneficial bacterium.

  14. Influence of Light and Temperature on Gene Expression Leading to Accumulation of Specific Flavonol Glycosides and Hydroxycinnamic Acid Derivatives in Kale (Brassica oleracea var. sabellica).

    PubMed

    Neugart, Susanne; Krumbein, Angelika; Zrenner, Rita

    2016-01-01

    Light intensity and temperature are very important signals for the regulation of plant growth and development. Plants subjected to less favorable light or temperature conditions often respond with accumulation of secondary metabolites. Some of these metabolites have been identified as bioactive compounds, considered to exert positive effects on human health when consumed regularly. In order to test a typical range of growth parameters for the winter crop Brassica oleracea var. sabellica, plants were grown either at 400 μmol m(-2) s(-1) or 100 μmol m(-2) s(-1) at 10°C, or at 400 μmol m(-2) s(-1) with 5 or 15°C. The higher light intensity overall increased flavonol content of leaves, favoring the main quercetin glycosides, a caffeic acid monoacylated kaempferol triglycoside, and disinapoyl-gentiobiose. The higher temperature mainly increased the hydroxycinnamic acid derivative disinapoyl-gentiobiose, while at lower temperature synthesis is in favor of very complex sinapic acid acylated flavonol tetraglycosides such as kaempferol-3-O-sinapoyl-sophoroside-7-O-diglucoside. A global analysis of light and temperature dependent alterations of gene expression in B. oleracea var. sabellica leaves was performed with the most comprehensive Brassica microarray. When compared to the light experiment much less genes were differentially expressed in kale leaves grown at 5 or 15°C. A structured evaluation of differentially expressed genes revealed the expected enrichment in the functional categories of e.g. protein degradation at different light intensities or phytohormone metabolism at different temperature. Genes of the secondary metabolism namely phenylpropanoids are significantly enriched with both treatments. Thus, the genome of B. oleracea was screened for predicted genes putatively involved in the biosynthesis of flavonoids and hydroxycinnamic acid derivatives. All identified B. oleracea genes were analyzed for their most specific 60-mer oligonucleotides present on the

  15. Influence of Light and Temperature on Gene Expression Leading to Accumulation of Specific Flavonol Glycosides and Hydroxycinnamic Acid Derivatives in Kale (Brassica oleracea var. sabellica)

    PubMed Central

    Neugart, Susanne; Krumbein, Angelika; Zrenner, Rita

    2016-01-01

    Light intensity and temperature are very important signals for the regulation of plant growth and development. Plants subjected to less favorable light or temperature conditions often respond with accumulation of secondary metabolites. Some of these metabolites have been identified as bioactive compounds, considered to exert positive effects on human health when consumed regularly. In order to test a typical range of growth parameters for the winter crop Brassica oleracea var. sabellica, plants were grown either at 400 μmol m−2 s−1 or 100 μmol m−2 s−1 at 10°C, or at 400 μmol m−2 s−1 with 5 or 15°C. The higher light intensity overall increased flavonol content of leaves, favoring the main quercetin glycosides, a caffeic acid monoacylated kaempferol triglycoside, and disinapoyl-gentiobiose. The higher temperature mainly increased the hydroxycinnamic acid derivative disinapoyl-gentiobiose, while at lower temperature synthesis is in favor of very complex sinapic acid acylated flavonol tetraglycosides such as kaempferol-3-O-sinapoyl-sophoroside-7-O-diglucoside. A global analysis of light and temperature dependent alterations of gene expression in B. oleracea var. sabellica leaves was performed with the most comprehensive Brassica microarray. When compared to the light experiment much less genes were differentially expressed in kale leaves grown at 5 or 15°C. A structured evaluation of differentially expressed genes revealed the expected enrichment in the functional categories of e.g. protein degradation at different light intensities or phytohormone metabolism at different temperature. Genes of the secondary metabolism namely phenylpropanoids are significantly enriched with both treatments. Thus, the genome of B. oleracea was screened for predicted genes putatively involved in the biosynthesis of flavonoids and hydroxycinnamic acid derivatives. All identified B. oleracea genes were analyzed for their most specific 60-mer oligonucleotides present on the

  16. Denudation rates determined from the accumulation of in situ-produced 10Be in the luquillo experimental forest, Puerto Rico

    USGS Publications Warehouse

    Brown, Erik Thorson; Stallard, Robert F.; Larsen, Matthew C.; Raisbeck, Grant M.; Yiou, Francoise

    1995-01-01

    We present a simple method for estimation of long-term mean denudation rates using in situ-produced cosmogenic 10Be in fluvial sediments. Procedures are discussed to account for the effects of soil bioturbation, mass wasting and attenuation of cosmic rays by biomass and by local topography. Our analyses of 10Be in quartz from bedrock outcrops, soils, mass-wasting sites and riverine sediment from the Icacos River basin in the Luquillo Experimental Forest, Puerto Rico, are used to characterize denudation for major landform elements in that basin. The 10Be concentration of a discharge-weighted average of size classes of river sediment corresponds to a long-term average denudation of ≈ 43 m Ma −1, consistent with mass balance results. 

  17. GPS-derived slip rates of active faults in eastern Venezuela, along the southeastern Caribbean PBZ

    NASA Astrophysics Data System (ADS)

    Audemard, F. A.; Beck, C.; Jouanne, F.; Reinoza, C. E.; Fegag

    2013-05-01

    For over 20 years, GPS campaign measurements have been performed in eastern Venezuela, as well as in other areas of the country, by different scientific groups and in the frame of different either national or international efforts and/or projects, essentially aiming at the estimation of the rate of motion along the major Quaternary faults (i.e., Boconó, San Sebastián and El Pilar faults) composing the plate boundary zone (PBZ) between the Caribbean and South America, along onshore northern and western Venezuela. The slip rates and sense of slip of those major faults derived from the comparison of several GPS campaigns carried out through the years have confirmed the slip data (fault kinematics) previously derived from geologic data, through comprehensive neotectonic and paleoseismic studies mainly made by the FUNVISIS' Earth Sciences Dpt. staff. In a rough way, we could conclude that those faults are dextrally moving at a rate in the order of 10-12 mm/a. More recently, it has been shown that the El Pilar fault has a locking depth close to 10 km deep and that about half of the PBZ dextral motion is accommodated as creep, reducing the seismic hazard for northeastern Venezuela almost by half. On the contrary, in the near past, very little attention has been paid to the secondary active faulting in eastern Venezuela. In that sense, FUNVISIS, in collaboration with the Université de Savoie, started the monitoring of these secondary features by installing 36 brass benchmarks on bedrock in that region in 2003, which have been occupied 3 times, in late 2003 and 2005 and in early 2013. The comparison between the 2003 and 2005 occupations shows promising results, such as: a) The Charagato fault on Cubagua island is left-lateral with a slip rate of about 2 mm/a; b) slip vectors across the El Pilar fault tend to head to the ESE, suggesting that the tectonic regime is compressive transcurrent to transcurrent compressional (transpressional); c) The NW-SE-trending San Francisco

  18. Locomotor response to exercise in relation to plasma lactate accumulation and heart rate in Andalusian and Anglo-Arabian horses.

    PubMed

    Muñoz, A; Santisteban, R; Rubio, M D; Riber, C; Agüera, E I; Castejón, F M

    1999-10-01

    To establish the effect of the locomotor pattern on heart rate (HR) and plasma lactate (LA), 28 horses, 18 Andalusian (AN) and 10 Anglo-Arabian (AA), aged 3-4 years, were studied. After a warm-up period, the horses performed a four-step test at 5, 6, 7 and 8 m/s, covering 1000 m at each velocity. HR was monitored, LA was analysed at rest and after each workload, and images were filmed. The locomotor parameters determined were stride duration (SD), frequency (SF) and length (SL), step and bipedal support durations, stance (restraint and propulsion) and swing phase durations, and stride vertical component. The HR and LA were significantly higher in the AN horses from velocities of 5 m/s. Similarly, the stride vertical component was higher in the AN horses at the trot and in the leading forelimbs at the gallop. Conversely, at all the galloping velocities, swing phase duration and stride length were longer in the AA horses. Significant correlations between HR, LA and locomotor pattern were only found in the AN horses. It was concluded that the greater stride vertical component in the AN horses limits SL and the cranial advancement of the hoof, with the result that reaching a longer SL triggers an increase in HR and LA. AA horses reach a balance between SL and SF, improving the efficiency of the gait.

  19. Partitioning and accumulation rates of polycyclic aromatic hydrocarbons into polydimethylsiloxane thin films and black worms from aqueous samples.

    PubMed

    Qin, Zhipei; Mok, Sandra; Ouyang, Gangfeng; Dixon, D George; Pawliszyn, Janusz

    2010-05-14

    Partition equilibriums and extraction rates of polycyclic aromatic hydrocarbons (PAHs) were examined for live biomonitoring with oligochaetes (black worms, Lumbriculus variegatus) and for high surface area chemical passive samplers constructed from polydimethylsiloxane thin film. The goals were to better understand the principles of bioconcentration by aquatic organisms and to aid in the design of a convenient and simple chemical monitoring tool to replace the use of live animals. The worms and films were exposed simultaneously to the contaminated water stream. In the initial extraction stage, similar extracted amount per surface area indicated that thin-film samplers could mimic the behavior of worms for passive sampling. Equilibrium was reached faster by the thin films than by the worms. A good linear relationship between the bioconcentration factors and the film-water partition coefficients of PAHs was found, which demonstrated the feasibility of thin-film sampler for determining the bioavailability of PAHs in water. Compared to the lengthy and inconvenient process of liquid-liquid extraction in worm treatment, thin-film technique simplifies the sample pretreatment procedure by integrating sampling and sample preparation.

  20. Effect of different tannery sludge compost amendment rates on growth, biomass accumulation and yield responses of Capsicum plants.

    PubMed

    Silva, Jayara D C; Leal, Tamara T B; Araújo, Ademir S F; Araujo, Raul M; Gomes, Regina L F; Melo, Wanderley J; Singh, Rajeev P

    2010-10-01

    Composting has been recognized as one of the most cost effective and environmentally sound alternatives for organic wastes recycling from long and composted wastes have a potential to substitute inorganic fertilizers. We investigated the potential of composted tannery sludge for ornamental purposes and to examine the effects of two different composts and concentrations on ornamental Capsicum growth. The two composts were produced with tannery sludge and the composition of each compost was: compost(1) of tannery sludge (C(1)TS) - tannery sludge+sugarcane straw and cattle manure mixed in the ratio 1:3:1 (v:v:v); compost(2) of tannery sludge (C(2)TS) - tannery sludge+"carnauba" straw and cattle manure in the ratio 1:3:1 (v:v:v). Each compost was amended with soil at rates (% v:v) of 0%, 25%, 50%, 75% and 100% (designation hereafter as T(1)-T(5), respectively). The number of leaves and fruits were counted, and the stem length was also measured. Chlorophyll content was recorded on three leaves of each harvested plant prior to harvest. Number of leaves and fruits, stem length, dry weight of shoot and roots did not vary significantly between the plants grown in two tannery composts. All the treatments with composted tannery sludge application (T(2)-T(5)) significantly increased the number of leaves and fruits, stem length and chlorophyll content compared with the control (T(1)). The chlorophyll content was higher in plants growing in the C(1)TS compared to C(2)TS. The results of the present study further suggest that Capsicum may be a good option to be grown on composted tannery amended soil.

  1. COMPARISON OF IN VIVO DERIVED AND SCALED IN VITRO METABOLIC RATE CONSTANTS FOR SOME VOLATILE ORGANIC COMPOUNDS (VOCS)

    EPA Science Inventory

    The reliability of physiologically based pharmacokinetic (PBPK) models is directly related to the accuracy of the metabolic rate parameters used as model inputs. When metabolic rate parameters derived from in vivo experiments are unavailable, they can be estimated from in vitro d...

  2. Derivation of a valid momentary first-order rate constant for kinetic and energetic analyses of enzymatic reactions.

    PubMed

    Imoto, Taiji

    2016-12-01

    To analyze enzymatic reactions energetically for comparison with non-enzymatic reactions (first order) under the same dimension, a method to derive valid momentary first-order rate constants for enzymatic reactions was developed. The momentary first-order rate constant, k enz0 = k cat[E'S']e,0/[S]0, was derived for an enzymatic reaction under a certain condition. It was shown that this rate constant is applicable for a wide range of enzymatic reactions. Utilizing this constant, one can conduct reliable kinetic and energetic analyses of enzymatic reactions.

  3. Accumulate-Repeat-Accumulate-Accumulate Codes

    NASA Technical Reports Server (NTRS)

    Divsalar, Dariush; Dolinar, Samuel; Thorpe, Jeremy

    2007-01-01

    Accumulate-repeat-accumulate-accumulate (ARAA) codes have been proposed, inspired by the recently proposed accumulate-repeat-accumulate (ARA) codes. These are error-correcting codes suitable for use in a variety of wireless data-communication systems that include noisy channels. ARAA codes can be regarded as serial turbolike codes or as a subclass of low-density parity-check (LDPC) codes, and, like ARA codes they have projected graph or protograph representations; these characteristics make it possible to design high-speed iterative decoders that utilize belief-propagation algorithms. The objective in proposing ARAA codes as a subclass of ARA codes was to enhance the error-floor performance of ARA codes while maintaining simple encoding structures and low maximum variable node degree.

  4. Acoustically derived growth rates of sperm whales (Physeter macrocephalus) in Kaikoura, New Zealand.

    PubMed

    Miller, Brian S; Growcott, Abraham; Slooten, Elisabeth; Dawson, Stephen M

    2013-09-01

    A non-invasive acoustic method for measuring the growth of sperm whales was developed based on estimating the length of individuals by measuring the inter-pulse interval (IPI) of their clicks. Most prior knowledge of growth in male sperm whales has come from from fitting growth curves to length data gained from whaling. Recordings made at Kaikoura, New Zealand, were used to estimate the length and growth of 32 photographically identified, resident whales that have been recorded repeatedly between 1991 and 2009. All whales recorded more than six months apart (n = 30) showed an increase in IPI. Using established relationships between IPI and total length, it was found that the average growth rate in the Kaikoura population is lower, especially for smaller whales (13-14.5 m), than that derived from historical whaling data from other populations. This difference may be due to ecological differences among populations but might also reflect upward bias in measurements gained in whaling. The ability to track growth of individuals through time is only possible via non-lethal means and offers a fundamentally different kind of data because differences among individuals can be measured.

  5. Glaciation in the Late Noachian Icy Highlands: Ice accumulation, distribution, flow rates, basal melting, and top-down melting rates and patterns

    NASA Astrophysics Data System (ADS)

    Fastook, James L.; Head, James W.

    2015-02-01

    Geological evidence for extensive non-polar ice deposits of Amazonian age indicates that the current cold and dry climate of Mars has persisted for several billion years. The geological record and climate history of the Noachian, the earliest period of Mars history, is less certain, but abundant evidence for fluvial channels (valley networks) and lacustrine environments (open-basin lakes) has been interpreted to represent warm and wet conditions, including rainfall and runoff. Alternatively, recent atmospheric modeling results predict a "cold and icy" Late Noachian Mars in which moderate atmospheric pressure accompanied by a full water cycle produce an atmosphere where temperature declines with elevation following an adiabatic lapse rate, in contrast to the current situation on Mars, where temperature is almost completely determined by latitude. These results are formulated in the Late Noachian Icy Highlands (LNIH) model, in which these cold and icy conditions lead to the preferential deposition of snow and ice at high elevations, such as the southern uplands. What is the fate of this snow and ice and the nature of glaciation in such an environment? What are the prospects of melting of these deposits contributing to the observed fluvial and lacustrine deposits? To address these questions, we report on a glacial flow-modeling analysis using a Mars-adapted ice sheet model with LNIH climate conditions. The total surface/near-surface water inventory is poorly known for the Late Noachian, so we explore the LNIH model in a "supply-limited" scenario for a range of available water abundances and a range of Late Noachian geothermal fluxes. Our results predict that the Late Noachian icy highlands (above an equilibrium line altitude of approximately +1 km) were characterized by extensive ice sheets of the order of hundreds of meters thick. Due to extremely cold conditions, the ice-flow velocities in general were very low, less than a few mm/yr, and the regional ice

  6. Snow Accumulation and Spring Melt Rates of Bogs and Fens in the North Granny Creek Catchment Basin, Hudson Bay Lowlands, Ontario

    NASA Astrophysics Data System (ADS)

    Cook, C. F.; Price, J. S.

    2009-05-01

    The Hudson Bay Lowlands contain one of the most extensive, contiguous peatland complexes in the world. Interlinked patterned peatlands developed in this region because of the cool climate, low-gradient topography and an underlying layer of low conductivity marine sediments. There is currently little research regarding the mechanisms that control runoff and surface water connectivity in this region, especially the functions of different peatland types on runoff production and flow pathways. Runoff generation in these systems is dependent on several factors such as soil and pool storage capacity, snow accumulation and melt rates, and peatland morphometry. Snowmelt accounts for a major portion of total annual runoff in this region and the timing of the melt will determine effective runoff production from a peatland catchment. One of the objectives of this project is to identify the processes and mechanisms that generate spring snowmelt runoff in different peatland types (i.e. bogs and fens) and quantify the relative contribution of each type in a peatland-dominated catchment basin. This research is being conducted in a 30 km2 catchment basin located near the DeBeers Victor diamond mine, located 90 km west of Attawapiskat, Ontario. The North Granny Creek basin is located approximately 3 km from the mine pit and is comprised of several peatland types and forms. The surface hydrology of this area is expected to be affected by groundwater depressurization due to dewatering of the mine pit by deep groundwater pumping wells. Effects of this activity on surface hydrology could possibly include increased soil storage capacity due to drier conditions and decreased melt rates due to reduced inputs of warm groundwater. Surface water connectivity is usually at a maximum in the spring because of a relatively impermeable frost table and low soil storage capacity which reduces infiltration. These effects of melt will not be observed uniformly over the entire catchment because of the

  7. Carbonic anhydrase activity and photosynthetic rate in the tree species Paulownia tomentosa Steud. Effect of dimethylsulfoxide treatment and zinc accumulation in leaves.

    PubMed

    Lazova, Galia N; Naidenova, Tsveta; Velinova, Katya

    2004-03-01

    The enzyme carbonic anhydrase (CA) (EC 4.2.1.1) catalyzes the reversible conversion of CO2 to HCO3- and has been shown to be involved in photosynthesis. The enzyme has been shown in animals, plants, eubacteria and viruses, but similar reports on the evidence for CA activity in tree plants does not be appear to be available. In the preliminary analyses of the work, the CA activity in leaf extracts from the tree species Paulownia tomentosa Steud. (introduced in Bulgaria) is described. A connection between CA activity and the rate of photosynthetic CO2 fixation is shown. In the second portion of the work, the effect of 10(-4) mol/L and 10(-2) mol/L dimethylsulfoxide (DMSO) on the zinc accumulation in leaves is demonstrated. It is suggested that CA activity is an indicator of the level of physiologically active zinc in leaves of P. tomentosa Steud. A connection between the process of zinc accumulation in leaves and the activity of the enzymes CA and glycolate oxidase (GO) (EC 1.1.3.1) is established.

  8. Pancreatic adenocarcinoma up-regulated factor (PAUF) enhances the accumulation and functional activity of myeloid-derived suppressor cells (MDSCs) in pancreatic cancer

    PubMed Central

    Jo, Seongyea; Kim, Yeon Jeong; Baek, Ji Eun; Kwon, Eun-Soo; Lee, Kwang-Pyo; Yang, Siyoung; Kwon, Ki-Sun; Kim, Dong-Uk; Kang, Tae Heung; Park, Yun-Yong; Chang, Suhwan; Cho, Hee Jun; Kim, Song Cheol; Koh, Sang Seok; Kim, Seokho

    2016-01-01

    Pancreatic cancer is characterized by an immunosuppressive tumor microenvironment (TME) with a profound immune infiltrate populated by a significant number of myeloid-derived suppressor cells (MDSCs). MDSCs have been increasingly recognized for their role in immune evasion and cancer progression as well as their potential as a target for immunotherapy. However, not much is known about the mechanisms regulating their behavior and function in the pancreatic TME. Here we report that pancreatic adenocarcinoma up-regulated factor (PAUF), a soluble protein involved in pancreatic tumorigenesis and metastasis, plays a role as an enhancer of tumor-infiltrating MDSC and its functional activity. We show that PAUF enhanced the accumulation of MDSCs in the spleen and tumor tissues of PAUF-overexpressing tumor cell-injected mice. In addition, PAUF was found to enhance the immunosuppressive function of MDSCs via the TLR4-mediated signaling pathway, which was demonstrated by PAUF-induced increased levels of arginase, nitric oxide (NO), and reactive oxygen species (ROS). The role of PAUF in modulating the functional properties of MDSCs was further demonstrated by the use of a PAUF-neutralizing antibody that caused a decreased number of tumor-infiltrating MDSCs and reduced MDSC immunosuppressive activity. The observations made in mice were confirmed in human pancreatic cancer patient-derived MDSCs, supporting the clinical relevance of our findings. Collectively, we conclude that the PAUF is a powerful and multifunctional promoter of tumor growth through increase and functional activation of MDSCs, suggesting therapeutic potential for targeting PAUF in pancreatic cancers. PMID:27322081

  9. Early and long-term mantle processing rates derived from xenon isotopes

    NASA Astrophysics Data System (ADS)

    Mukhopadhyay, S.; Parai, R.; Tucker, J.; Middleton, J. L.; Langmuir, C. H.

    2015-12-01

    Noble gases, particularly xenon (Xe), in mantle-derived basalts provide a rich portrait of mantle degassing and surface-interior volatile exchange. The combination of extinct and extant radioactive species in the I-Pu-U-Xe systems shed light on the degassing history of the early Earth throughout accretion, as well as the long-term degassing of the Earth's interior in association with plate tectonics. The ubiquitous presence of shallow-level air contamination, however, frequently obscures the mantle Xe signal. In a majority of the samples, shallow air contamination dominates the Xe budget. For example, in the gas-rich popping rock 2ΠD43, 129Xe/130Xe ratios reach 7.7±0.23 in individual step-crushes, but the bulk composition of the sample is close to air (129Xe/130Xe of 6.7). Thus, the extent of variability in mantle source Xe composition is not well-constrained. Here, we present new MORB Xe data and explore constraints placed on mantle processing rates by the Xe data. Ten step-crushes were obtained on a depleted popping glass that was sealed in ultrapure N2 after dredge retrieval from between the Kane-Atlantis Fracture Zone of the Mid Atlantic Ridge in May 2012. 9 steps yielded 129Xe/130Xe of 7.50-7.67 and one yielded 7.3. The bulk 129Xe/130Xe of the sample is 7.6, nearly identical to the estimated mantle source value of 7.7 for the sample. Hence, the sample is virtually free of shallow-level air contamination. Because sealing the sample in N2upon dredge retrieval largely eliminated air contamination, for many samples, contamination must be added after sample retrieval from the ocean bottom. Our new high-precision Xe isotopic measurements in upper mantle-derived samples provide improved constraints on the Xe isotopic composition of the mantle source. We developed a forward model of mantle volatile evolution to identify solutions that satisfy our Xe isotopic data. We find that accretion timescales of ~10±5 Myr are consistent with I-Pu-Xe constraints, and the last

  10. Specific Accumulation of Tumor-Derived Adhesion Factor in Tumor Blood Vessels and in Capillary Tube-Like Structures of Cultured Vascular Endothelial Cells

    NASA Astrophysics Data System (ADS)

    Akaogi, Kotaro; Okabe, Yukie; Sato, Junji; Nagashima, Yoji; Yasumitsu, Hidetaro; Sugahara, Kazuyuki; Miyazaki, Kaoru

    1996-08-01

    Tumor-derived adhesion factor (TAF) was previously identified as a cell adhesion molecule secreted by human bladder carcinoma cell line EJ-1. To elucidate the physiological function of TAF, we examined its distribution in human normal and tumor tissues. Immunochemical staining with an anti-TAF monoclonal antibody showed that TAF was specifically accumulated in small blood vessels and capillaries within and adjacent to tumor nests, but not in those in normal tissues. Tumor blood vessel-specific staining of TAF was observed in various human cancers, such as esophagus, brain, lung, and stomach cancers. Double immunofluorescent staining showed apparent colocalization of TAF and type IV collagen in the vascular basement membrane. In vitro experiments demonstrated that TAF preferentially bound to type IV collagen among various extracellular matrix components tested. In cell culture experiments, TAF promoted adhesion of human umbilical vein endothelial cells to type IV collagen substrate and induced their morphological change. Furthermore, when the endothelial cells were induced to form capillary tube-like structures by type I collagen, TAF and type IV collagen were exclusively detected on the tubular structures. The capillary tube formation in vitro was prevented by heparin, which inhibited the binding of TAF to the endothelial cells. These results strongly suggest that TAF contributes to the organization of new capillary vessels in tumor tissues by modulating the interaction of endothelial cells with type IV collagen.

  11. Synergistic infection of BrYV and PEMV 2 increases the accumulations of both BrYV and BrYV-derived siRNAs in Nicotiana benthamiana

    PubMed Central

    Zhou, Cui-Ji; Zhang, Xiao-Yan; Liu, Song-Yu; Wang, Ying; Li, Da-Wei; Yu, Jia-Lin; Han, Cheng-Gui

    2017-01-01

    Viral synergism is caused by co-infection of two unrelated viruses, leading to more severe symptoms or increased titres of one or both viruses. Synergistic infection of phloem-restricted poleroviruses and umbraviruses has destructive effects on crop plants. The mechanism underlying this synergy remains elusive. In our study, synergism was observed in co-infections of a polerovirus Brassica yellows virus (BrYV) and an umbravirus Pea enation mosaic virus 2 (PEMV 2) on Nicotiana benthamiana, which led to (1) increased titres of BrYV, (2) appearance of severe symptoms, (3) gain of mechanical transmission capacity of BrYV, (4) broader distribution of BrYV to non-vascular tissues. Besides, profiles of virus-derived small interfering RNAs (vsiRNAs) from BrYV and PEMV 2 in singly and doubly infected plants were obtained by small RNA deep sequencing. Our results showed that accumulation of BrYV vsiRNAs increased tremendously and ratio of positive to negative strand BrYV vsiRNAs differed between singly infected and co-infected plants. Positions to which the BrYV vsiRNAs mapped to the viral genome varied considerably during synergistic infection. Moreover, target genes of vsiRNAs were predicted and annotated. Our results revealed the synergistic characteristics during co-infection of BrYV and PEMV 2, and implied possible effects of synergism have on vsiRNAs. PMID:28345652

  12. Estimating the effects of 17α-ethinylestradiol on stochastic population growth rate of fathead minnows: a population synthesis of empirically derived vital rates.

    PubMed

    Schwindt, Adam R; Winkelman, Dana L

    2016-09-01

    Urban freshwater streams in arid climates are wastewater effluent dominated ecosystems particularly impacted by bioactive chemicals including steroid estrogens that disrupt vertebrate reproduction. However, more understanding of the population and ecological consequences of exposure to wastewater effluent is needed. We used empirically derived vital rate estimates from a mesocosm study to develop a stochastic stage-structured population model and evaluated the effect of 17α-ethinylestradiol (EE2), the estrogen in human contraceptive pills, on fathead minnow Pimephales promelas stochastic population growth rate. Tested EE2 concentrations ranged from 3.2 to 10.9 ng L(-1) and produced stochastic population growth rates (λ S ) below 1 at the lowest concentration, indicating potential for population decline. Declines in λ S compared to controls were evident in treatments that were lethal to adult males despite statistically insignificant effects on egg production and juvenile recruitment. In fact, results indicated that λ S was most sensitive to the survival of juveniles and female egg production. More broadly, our results document that population model results may differ even when empirically derived estimates of vital rates are similar among experimental treatments, and demonstrate how population models integrate and project the effects of stressors throughout the life cycle. Thus, stochastic population models can more effectively evaluate the ecological consequences of experimentally derived vital rates.

  13. The influence of structural features of marine humic substances on the accumulation rates of cadmium by a blue mussel Mytilus edulis

    SciTech Connect

    Pempkowiak, J.; Kozuch, J. ); Southon, T. )

    1994-01-01

    Laboratory experiments revealed that both concentration and origin of humic substances (HS) influence the accumulation rates of cadmium by the blue mussel Mytilus edulis. In the concentration of humic substances typical of seawater, the increase is about 60% and 100%, respectively, for aquatic and sedimentary humic substances. The phenomenon was attributed to the stimulation of cadmium uptake due to complexing properties of the substances toward cadmium. Complexing capacity of sedimentary humic substances was found to be 0.57 [mu]g/mg HS, that of aquatic substances 0.41 [mu]g/mg HS. Cross Polarization Magic Angle Spinning (CP/MAS) [sup 13]C NMR of the investigated humic substances revealed differences in the spectra at about 175, 100, 55 and 32 ppm. This was attributed to the varying content of oxygen containing functional groups involved in formation of complexes with metal ions. 8 refs., 4 figs., 3 tabs.

  14. Spatial and temporal variations in denudation rates derived from cosmogenic nuclides in four European fluvial terrace sequences

    NASA Astrophysics Data System (ADS)

    Schaller, M.; Ehlers, T. A.; Stor, T.; Torrent, J.; Lobato, L.; Christl, M.; Vockenhuber, C.

    2016-12-01

    The denudation of landscapes is affected by temporal and spatial variations in tectonics, climate, and vegetation. However, deciphering the contributions of these different processes has proven challenging. In this study, cosmogenic nuclide-derived modern and paleo catchment-wide denudation rates in four European rivers are investigated. We present 12 new and 4 recalculated cosmogenic nuclide-derived denudation rates from modern river sediments and 14 paleo-denudation rates from terraces deposited over the last 2 Ma. The catchments studied are located in regions with minimal Quaternary tectonic activity and span different climates over 12o latitude. Results indicate that modern denudation rates range between 16 ± 11 and 51 ± 7 mm/ka with no clear latitudinal variation. Modern denudation rates are compared with catchment geomorphic indices including slope, fluvial steepness index, and relief. The denudation rates correlate better to catchment topographic indices (R2 ≈ 0.4) rather than climate. Paleo-denudation rates range from 8 ± 7 to 56 ± 7 mm/ka and are associated with a possible increase in the average paleo-denudation rates over the past 2 Ma. Taken together, the results indicate that quantification of catchment-wide denudation rates over long (Quaternary) time scales because of climate change is difficult. Future work to study climate influence on denudation rates should focus on the successes of previous work that document transient denudation rates over shorter and more recent time scales, i.e., from the Last Glacial Maximum to present.

  15. Accumulation of gamma-globin mRNA and induction of irreversible erythroid differentiation after treatment of CML cell line K562 with new doxorubicin derivatives.

    PubMed

    Szulawska, Agata; Arkusinska, Justyna; Czyz, Malgorzata

    2007-01-15

    Human chronic myelogenous leukemia (CML) cell line K562 can be chemically induced to differentiate and express embryonic and fetal globin genes. In this study, the effects of doxorubicin (DOX), an inducer of K562 cell erythroid differentiation, with those of epidoxorubicin (EDOX) as well as newly synthesized derivatives of both drugs (DOXM, DOXH, and EDOXM) on cell growth and differentiation were compared. Our results revealed that DOX, EDOX and their derivatives caused irreversible differentiation of K562 cells into more mature hemoglobin-containing cells. This phenomenon was linked to time-dependent inhibition of cell proliferation. Considering the impact of the structure of newly synthesized anthracyclines on their cellular activity, our data clearly indicated that among tested anthracyclines DOXM, a morpholine derivative of DOX exerted the highest antiproliferative and differentiating activity. An increase of gamma-globin mRNA level caused both by high transcription rate and by mRNA stabilization, as well as an enhancement of expression but not activity of erythroid transcription factor GATA-1 were observed. Therefore, a high level of hemoglobin-containing cells in the presence of DOXM resulted from transcriptional and post-transcriptional events on gamma-globin gene regulation. The same morpholine modification introduced to EDOX did not cause, however, similar effects on cellular level. Characterization of new powerful inducers of erythroid differentiation may contribute to the development of novel compounds for pharmacological approach by differentiation therapy to leukemia or to beta-globin disorder, beta-thalassemia.

  16. Lava Discharge Rates at Kīlauea Volcano, Hawai'I, during 2011-2013 Determined from Tandem-X-Derived Topographic Data

    NASA Astrophysics Data System (ADS)

    Poland, M. P.

    2014-12-01

    The effusion rate of lava from a basaltic volcano is a parameter of critical importance given its direct association with hazard—for example, high effusion rates imply long lava flows. At Kīlauea Volcano, Hawai'i, numerous methods have been used to quantify effusion rate, including direct observation of lava streams, measurement of gas emissions, geologic mapping, modeling of thermal radiance, and quantification of topographic change. None of these techniques, however, have consistently yielded reliable results since about 2008, due to either changes in the character of volcanism or the expense associated with data collection. Synthetic Aperture Radar (SAR) data from the TanDEM-X satellite mission offer a potential solution to this problem. Differencing digital elevation models (DEMs) derived from temporally sequential TanDEM-X SAR imagery provides measure of elevation change over time due to accumulation of lava across the entirety of Kīlauea's 100 km2 East Rift Zone lava flow field. Summing these elevation changes over the area of an active lava flow and dividing by the time spanned by the TanDEM-X data gives the time-averaged discharge rate (TADR) of lava. The TADR calculated from multiple TanDEM-X-derived DEMs spanning days to weeks at Kīlauea during mid-2011 to mid-2013 suggests a dense-rock equivalent lava discharge rate of approximately 2 m3/s, which is about half the long-term average rate over the course of Kīlauea's 1983-present East Rift Zone eruption. This result implies an increase in the rate of lava storage at Kīlauea, a decrease in the magma supply rate, or some combination of both with respect to previous years. TanDEM-X-derived topographic maps also provide insights into the four-dimensional growth of Kīlauea's lava flow field—a dataset not available by other means but important for assessing the factors that control current and future lava flow pathways.

  17. Hydraulic conductance as well as nitrogen accumulation plays a role in the higher rate of leaf photosynthesis of the most productive variety of rice in Japan

    PubMed Central

    Taylaran, Renante D.; Adachi, Shunsuke; Ookawa, Taiichiro; Usuda, Hideaki; Hirasawa, Tadashi

    2011-01-01

    An indica variety Takanari is known as one of the most productive rice varieties in Japan and consistently produces 20–30% heavier dry matter during ripening than Japanese commercial varieties in the field. The higher rate of photosynthesis of individual leaves during ripening has been recognized in Takanari. By using pot-grown plants under conditions of minimal mutual shading, it was confirmed that the higher rate of leaf photosynthesis is responsible for the higher dry matter production after heading in Takanari as compared with a japonica variety, Koshihikari. The rate of leaf photosynthesis and shoot dry weight became larger in Takanari after the panicle formation and heading stages, respectively, than in Koshihikari. Roots grew rapidly in the panicle formation stage until heading in Takanari compared with Koshihikari. The higher rate of leaf photosynthesis in Takanari resulted not only from the higher content of leaf nitrogen, which was caused by its elevated capacity for nitrogen accumulation, but also from higher stomatal conductance. When measured under light-saturated conditions, stomatal conductance was already decreased due to the reduction in leaf water potential in Koshihikari even under conditions of a relatively small difference in leaf–air vapour pressure difference. In contrast, the higher stomatal conductance was supported by the maintenance of higher leaf water potential through the higher hydraulic conductance in Takanari with the larger area of root surface. However, no increase in root hydraulic conductivity was expected in Takanari. The larger root surface area of Takanari might be a target trait in future rice breeding for increasing dry matter production. PMID:21527630

  18. Hydraulic conductance as well as nitrogen accumulation plays a role in the higher rate of leaf photosynthesis of the most productive variety of rice in Japan.

    PubMed

    Taylaran, Renante D; Adachi, Shunsuke; Ookawa, Taiichiro; Usuda, Hideaki; Hirasawa, Tadashi

    2011-07-01

    An indica variety Takanari is known as one of the most productive rice varieties in Japan and consistently produces 20-30% heavier dry matter during ripening than Japanese commercial varieties in the field. The higher rate of photosynthesis of individual leaves during ripening has been recognized in Takanari. By using pot-grown plants under conditions of minimal mutual shading, it was confirmed that the higher rate of leaf photosynthesis is responsible for the higher dry matter production after heading in Takanari as compared with a japonica variety, Koshihikari. The rate of leaf photosynthesis and shoot dry weight became larger in Takanari after the panicle formation and heading stages, respectively, than in Koshihikari. Roots grew rapidly in the panicle formation stage until heading in Takanari compared with Koshihikari. The higher rate of leaf photosynthesis in Takanari resulted not only from the higher content of leaf nitrogen, which was caused by its elevated capacity for nitrogen accumulation, but also from higher stomatal conductance. When measured under light-saturated conditions, stomatal conductance was already decreased due to the reduction in leaf water potential in Koshihikari even under conditions of a relatively small difference in leaf-air vapour pressure difference. In contrast, the higher stomatal conductance was supported by the maintenance of higher leaf water potential through the higher hydraulic conductance in Takanari with the larger area of root surface. However, no increase in root hydraulic conductivity was expected in Takanari. The larger root surface area of Takanari might be a target trait in future rice breeding for increasing dry matter production.

  19. Modeling Plant Uptake of Metal in Constructed Wetlands Supported by Experimentally Derived Uptake Rates.

    DTIC Science & Technology

    1996-12-01

    stress to a system and could eventually induce chronic effects such as decreased species diversity and biomass , altered biogeochemical nutrient...erosion Remove accumulated solids in forebay and near outlet Clean pretreatment devices; clean forebay once every five years or when sediment exceeds...Sagua la Grande river basin near a chlor- alkali plant and other industrial plants differed by location. Highest concentrations were found in the zone

  20. Late Quaternary sediment-accumulation rates within the inner basins of the California Continental Borderland in support of geologic hazard evaluation

    USGS Publications Warehouse

    Normark, W.R.; McGann, M.; Sliter, R.W.

    2009-01-01

    An evaluation of the geologic hazards of the inner California Borderland requires determination of the timing for faulting and mass-movement episodes during the Holocene. Our effort focused on basin slopes and turbidite systems on the basin floors for the area between Santa Barbara and San Diego, California. Dating condensed sections on slopes adjacent to fault zones provides better control on fault history where high-resolution, seismic-reflection data can be used to correlate sediment between the core site and the fault zones. This study reports and interprets 147 radiocarbon dates from 43 U.S. Geological Survey piston cores as well as 11 dates from Ocean Drilling Program Site 1015 on the floor of Santa Monica Basin. One hundred nineteen dates from 39 of the piston cores have not previously been published. Core locations were selected for hazard evaluation, but despite the nonuniform distribution of sample locations, the dates obtained for the late Quaternary deposits are useful for documenting changes in sediment-accumulation rates during the past 30 ka. Cores from basins receiving substantial sediment from rivers, i.e., Santa Monica Basin and the Gulf of Santa Catalina, show a decrease in sediment supply during the middle Holocene, but during the late Holocene after sea level had reached the current highstand condition, rates then increased partly in response to an increase in El Ni??o-Southern Oscillation events during the past 3.5 ka. ?? 2009 The Geological Society of America.

  1. Solar Energy Deposition Rates in the Mesosphere Derived from Airglow Measurements: Implications for the Ozone Model Deficit Problem

    NASA Technical Reports Server (NTRS)

    Mlynczak, Martin G.; Garcia, Rolando R.; Roble, Raymond G.; Hagan, Maura

    2000-01-01

    We derive rates of energy deposition in the mesosphere due to the absorption of solar ultraviolet radiation by ozone. The rates are derived directly from measurements of the 1.27-microns oxygen dayglow emission, independent of knowledge of the ozone abundance, the ozone absorption cross sections, and the ultraviolet solar irradiance in the ozone Hartley band. Fifty-six months of airglow data taken between 1982 and 1986 by the near-infrared spectrometer on the Solar-Mesosphere Explorer satellite are analyzed. The energy deposition rates exhibit altitude-dependent annual and semi-annual variations. We also find a positive correlation between temperatures and energy deposition rates near 90 km at low latitudes. This correlation is largely due to the semiannual oscillation in temperature and ozone and is consistent with model calculations. There is also a suggestion of possible tidal enhancement of this correlation based on recent theoretical and observational analyses. The airglow-derived rates of energy deposition are then compared with those computed by multidimensional numerical models. The observed and modeled deposition rates typically agree to within 20%. This agreement in energy deposition rates implies the same agreement exists between measured and modeled ozone volume mixing ratios in the mesosphere. Only in the upper mesosphere at midlatitudes during winter do we derive energy deposition rates (and hence ozone mixing ratios) consistently and significantly larger than the model calculations. This result is contrary to previous studies that have shown a large model deficit in the ozone abundance throughout the mesosphere. The climatology of solar energy deposition and heating presented in this paper is available to the community at the Middle Atmosphere Energy Budget Project web site at http://heat-budget.gats-inc.com.

  2. Validation of Empirically Derived Rating Scales for a Story Retelling Speaking Test

    ERIC Educational Resources Information Center

    Hirai, Akiyo; Koizumi, Rie

    2013-01-01

    In recognition of the rating scale as a crucial tool of performance assessment, this study aims to establish a rating scale suitable for a Story Retelling Speaking Test (SRST), which is a semidirect test of speaking ability in English as a foreign language for classroom use. To identify an appropriate scale, three rating scales, all of which have…

  3. Summary of information on low-speed lateral-directional derivatives due to rate of change of sideslip beta prime

    NASA Technical Reports Server (NTRS)

    Coe, P. L., Jr.; Graham, A. B.; Chambers, J. R.

    1975-01-01

    The results presented show that the magnitudes of the aerodynamic stability derivatives due to rate of change of sideslip become quite large at high angles of attack for swept- and delta-wing configurations, and that such derivatives have large effects on the calculated dynamic stability of these configurations at high angles of attack. The wind-tunnel test techniques used to measure the beta prime derivatives and various approaches used to predict them are discussed. Both the conventional oscillating-airfoil theory and the lag-of-the-sidewash theory are shown to be inadequate for predicting the vertical-tail contribution to the acceleration-in-sideslip derivative; a flow-field-lag theory, which is discussed, appears to give qualitative agreement with experimental data for a current twin-jet fighter configuration.

  4. Growth Kinetics and Morphology of Barite Crystals Derived from Face-Specific Growth Rates

    SciTech Connect

    Godinho, Jose R. A.; Stack, Andrew G.

    2015-03-30

    Here we investigate the growth kinetics and morphology of barite (BaSO4) crystals by measuring the growth rates of the (001), (210), (010), and (100) surfaces using vertical scanning interferometry. Solutions with saturation indices 1.1, 2.1, and 3.0 without additional electrolyte, in 0.7 M NaCl, or in 1.3 mM SrCl2 are investigated. Face-specific growth rates are inhibited in the SrCl2 solution relative to a solution without electrolyte, except for (100). Contrarily, growth of all faces is promoted in the NaCl solution. The variation of face-specific rates is solution-specific, which leads to a. change of the crystal morphology and overall growth rate of crystals. The measured face-specific growth rates are used to model the growth of single crystals. Modeled crystals have a morphology and size similar to those grown from solution. Based on the model the time dependence of surface area and growth rates is analyzed. Growth rates change with time due to surface area normalization for small crystals and large growth intervals. By extrapolating rates to crystals with large surfaces areas, time-independent growth rates are 0.783, 2.96, and 0.513 mmol∙m-2∙h-1, for saturation index 2.1 solutions without additional electrolyte, NaCl, and SrCl2, respectively.

  5. Growth Kinetics and Morphology of Barite Crystals Derived from Face-Specific Growth Rates

    DOE PAGES

    Godinho, Jose R. A.; Stack, Andrew G.

    2015-03-30

    Here we investigate the growth kinetics and morphology of barite (BaSO4) crystals by measuring the growth rates of the (001), (210), (010), and (100) surfaces using vertical scanning interferometry. Solutions with saturation indices 1.1, 2.1, and 3.0 without additional electrolyte, in 0.7 M NaCl, or in 1.3 mM SrCl2 are investigated. Face-specific growth rates are inhibited in the SrCl2 solution relative to a solution without electrolyte, except for (100). Contrarily, growth of all faces is promoted in the NaCl solution. The variation of face-specific rates is solution-specific, which leads to a. change of the crystal morphology and overall growth ratemore » of crystals. The measured face-specific growth rates are used to model the growth of single crystals. Modeled crystals have a morphology and size similar to those grown from solution. Based on the model the time dependence of surface area and growth rates is analyzed. Growth rates change with time due to surface area normalization for small crystals and large growth intervals. By extrapolating rates to crystals with large surfaces areas, time-independent growth rates are 0.783, 2.96, and 0.513 mmol∙m-2∙h-1, for saturation index 2.1 solutions without additional electrolyte, NaCl, and SrCl2, respectively.« less

  6. Carbon accumulation in a permafrost polygon peatland: steady long-term rates in spite of shifts between dry and wet conditions.

    PubMed

    Gao, Yang; Couwenberg, John

    2015-02-01

    Ice-wedge polygon peatlands contain a substantial part of the carbon stored in permafrost soils. However, little is known about their long-term carbon accumulation rates (CAR) in relation to shifts in vegetation and climate. We collected four peat profiles from one single polygon in NE Yakutia and cut them into contiguous 0.5 cm slices. Pollen density interpolation between AMS (14)C dated levels provided the time span contained in each of the sample slices, which--in combination with the volumetric carbon content--allowed for the reconstruction of CAR over decadal and centennial timescales. Vegetation representing dry palaeo-ridges and wet depressions was reconstructed with detailed micro- and macrofossil analysis. We found repeated shifts between wet and dry conditions during the past millennium. Dry ridges with associated permafrost growth originated during phases of (relatively) warm summer temperature and collapsed during relatively cold phases, illustrating the important role of vegetation and peat as intermediaries between ambient air temperature and the permafrost. The average long-term CAR across the four profiles was 10.6 ± 5.5 g C m(-2) yr(-1). Time-weighted mean CAR did not differ significantly between wet depression and dry ridge/hummock phases (10.6 ± 5.2 g C m(-2) yr(-1) and 10.3 ± 5.7 g C m(-2) yr(-1), respectively). Although we observed increased CAR in relation to warm shifts, we also found changes in the opposite direction and the highest CAR actually occurred during the Little Ice Age. In fact, CAR rather seems to be governed by strong internal feedback mechanisms and has roughly remained stable on centennial time scales. The absence of significant differences in CAR between dry ridge and wet depression phases suggests that recent warming and associated expansion of shrubs will not affect long-term rates of carbon burial in ice-wedge polygon peatlands.

  7. Pulse oximetry-derived respiratory rate in general care floor patients.

    PubMed

    Addison, Paul S; Watson, James N; Mestek, Michael L; Ochs, James P; Uribe, Alberto A; Bergese, Sergio D

    2015-02-01

    Respiratory rate is recognized as a clinically important parameter for monitoring respiratory status on the general care floor (GCF). Currently, intermittent manual assessment of respiratory rate is the standard of care on the GCF. This technique has several clinically-relevant shortcomings, including the following: (1) it is not a continuous measurement, (2) it is prone to observer error, and (3) it is inefficient for the clinical staff. We report here on an algorithm designed to meet clinical needs by providing respiratory rate through a standard pulse oximeter. Finger photoplethysmograms were collected from a cohort of 63 GCF patients monitored during free breathing over a 25-min period. These were processed using a novel in-house algorithm based on continuous wavelet-transform technology within an infrastructure incorporating confidence-based averaging and logical decision-making processes. The computed oximeter respiratory rates (RRoxi) were compared to an end-tidal CO2 reference rate (RRETCO2). RRETCO2 ranged from a lowest recorded value of 4.7 breaths per minute (brpm) to a highest value of 32.0 brpm. The mean respiratory rate was 16.3 brpm with standard deviation of 4.7 brpm. Excellent agreement was found between RRoxi and RRETCO2, with a mean difference of -0.48 brpm and standard deviation of 1.77 brpm. These data demonstrate that our novel respiratory rate algorithm is a potentially viable method of monitoring respiratory rate in GCF patients. This technology provides the means to facilitate continuous monitoring of respiratory rate, coupled with arterial oxygen saturation and pulse rate, using a single non-invasive sensor in low acuity settings.

  8. Temperature-dependent growth rates and gene expression patterns of various medaka Oryzias latipes cell lines derived from different populations.

    PubMed

    Hirayama, Makoto; Mitani, Hiroshi; Watabe, Shugo

    2006-05-01

    Medaka Oryzias latipes has several geographically and genetically distinct populations. We examined temperature acclimation response in various medaka cell lines derived from different populations. Measurement of cell growth at various temperatures suggested that 15 degrees Celsius was the permissive growth temperature in all cell lines from the Northern Japanese and East Korean populations, but not in those from the Southern Japanese population and medaka-related species Oryzias celebensis, which inhabits a tropical zone. RT-PCR for 102 temperature-responsive genes, previously reported in other species, revealed that the accumulated mRNA level of a gene encoding HSP47 was lower at 25 degrees Celsius than at 33 degrees Celsius, and vice versa for 12 genes including IkappaBalpha and Rab-1c, in OLHNI-1 cell line from the Northern Japanese population. Further analysis by real-time PCR demonstrated that the accumulated mRNA levels of IkappaBalpha and Rab-1c in OLHNI-1 and OLSOK-e7 cell lines from the East Korean population were increased when the culture temperature was shifted from 33 to 15 degrees Celsius, but not in OLHdrR-e3 cell line from the Southern Japanese population. Since IkappaBalpha and Rab-1c are related to the NFkappaB cascade and endoplasmic reticulum-to-Golgi transport, respectively, it is inferred that immune responses and intracellular transport are possibly critical to temperature adaptation for medaka.

  9. Influence of topography and human activity on apparent in situ 10Be-derived erosion rates in Yunnan, SW China

    NASA Astrophysics Data System (ADS)

    Schmidt, Amanda H.; Neilson, Thomas B.; Bierman, Paul R.; Rood, Dylan H.; Ouimet, William B.; Sosa Gonzalez, Veronica

    2016-11-01

    In order to understand better if and where erosion rates calculated using in situ 10Be are affected by contemporary changes in land use and attendant deep regolith erosion, we calculated erosion rates using measurements of in situ 10Be in quartz from 52 samples of river sediment collected from three tributaries of the Mekong River (median basin area = 46.5 km2). Erosion rates range from 12 to 209 mm kyr-1 with an area-weighted mean of 117 ± 49 mm kyr-1 (1 standard deviation) and median of 74 mm kyr-1. We observed a decrease in the relative influence of human activity from our steepest and least altered watershed in the north to the most heavily altered landscapes in the south. In the areas of the landscape least disturbed by humans, erosion rates correlate best with measures of topographic steepness. In the most heavily altered landscapes, measures of modern land use correlate with 10Be-estimated erosion rates but topographic steepness parameters cease to correlate with erosion rates. We conclude that, in some small watersheds with high rates and intensity of agricultural land use that we sampled, tillage and resultant erosion has excavated deeply enough into the regolith to deliver subsurface sediment to streams and thus raise apparent in situ 10Be-derived erosion rates by as much as 2.5 times over background rates had the watersheds not been disturbed.

  10. Modern accumulation rates and a budget of sediment off the Gaoping (Kaoping) River, SW Taiwan: A tidal and flood dominated depositional environment around a submarine canyon

    NASA Astrophysics Data System (ADS)

    Huh, Chih-An; Lin, Hui-Ling; Lin, Saulwood; Huang, Ya-Wen

    2009-03-01

    Ninety-two box cores collected during 2004-2006 from an area of ~ 3000 km 2 off the Gaoping (formerly spelled Kaoping) River, SW Taiwan, were analyzed for fallout radionuclides ( 210Pb, 137Cs and 7Be) to elucidate sedimentation rates and processes, and for the calculation of a sediment budget. The study area is located at an active collision margin with a narrow shelf and a submarine canyon extending essentially into the river's mouth. The results indicate fairly constant hemipelagic sedimentation in much of the open margin and for most of the time except in the inner shelf and along the axis of the canyon where sediment transport is more dynamic and is controlled by tidal current and wave activities constantly, and by fluvial floods or gravity-driven flows episodically. Sedimentation rates in the study area derived from 210Pb and constrained by 137Cs vary from 0.04 to 1.5 cm/yr, with the highest rates (> 1 cm/yr) flanking the Gaoping canyon over the upper slope (200-600 m) and the lowest rates (< 0.1 cm/yr) in the distal basin beyond the continental slope. The depocenter delineated from 210Pb-based sedimentation rates overlaps with the area covered by a flood layer resulting from super-typhoon Haitang in July 2005. Such correspondence supports the notion that the processes operating on event timescale have bearing on the formation of the sediment strata over centennial or longer timescales. From the distribution of sedimentation rates, sediment deposited in the study area annually is estimated to be 6.6 Mton/yr, accounting for less than 20% of Gaoping River's sediment load. The calculated budget, coupled with the presence of the short-lived 7Be and non-steady-state distribution of low levels of 210Pb in sediments along the canyon floor, suggests rapid transport of sediment from Gaoping River's mountainous watershed (the source) via the Gaoping (Kaoping) Submarine Canyon and adjacent channels (as the conduit and temporary sink) to the abyssal plain and the Manila

  11. A record of barite accumulation rate for marine export productivity changes in the tropical Indian Ocean during the Mid-Pliocene--Early-Pleistocene transition

    NASA Astrophysics Data System (ADS)

    Zhou, Liping; Ma, Zhongwu; Ding, Xuan

    2016-04-01

    One of the most interesting features in the marine oxygen isotope records is the gradual shift towards heavier 18O from the Mid-Pliocene, which ends with the initiation of Northern Hemisphere glaciation (NHG) around 2.7 Ma. The lack of significant change in sea surface temperature in the tropical Indian Ocean as revealed in the previous studies does not rule out their possible contributions to this dramatic climate change during the Mid-Pliocene transition. Changing circulation systems in the region will control the supply of nutrients for the water masses which in turn determine the marine productivity. In the areas of high productivity, ocean export productivity may potentially provide a mechanism of CO2 draw-down into the deep ocean, through which contributing to the lowering of the global temperature. In this study, we present a record of barite accumulation rate (BAR) for DSDP Site 214 drilled on the Ninetyeast Ridge. Here we use the marine barite, which is formed during the decay of organism in the twilight zone, as a proxy for ocean export productivity. Our results show that the BAR of Site 214 varies between 0.25 and 1.25 mg/cm2/kyr during the period between 4 Ma and 2 Ma. Five intervals of increased BAR from 3.6 Ma to 2.4 Ma are identified with the most distinct peak centred around 3 Ma. The overall pattern does not follow either the oxygen isotope record for the Site or the sea surface temperature and subsurface temperature reconstructed with the Mg/Ca of foraminifera. This suggests that regional changes in ocean circulation and water masses may have played more important role than temperature in controlling the productivity change in the tropical Indian Ocean. The relative higher productivity around 3 Ma may imply a biogenetic process towards the intensification of NHGs.

  12. Erosion rates along fault scarps and rift-shoulder environments in central and northern Kenya: Insights from new 10Be-derived basin-wide erosion rates

    NASA Astrophysics Data System (ADS)

    Torres-Acosta, V.; Strecker, M. R.; Schildgen, T. F.; Wittmann, H.; Scherler, D.; Bookhagen, B.

    2011-12-01

    The Kenya Rift is typical example of an active continental rift zone and is a fundamental part of the East African Rift system. The rift valley plays a central role in archiving the relationships between sedimentation, erosion, and climate in the region. However, the links between surface processes (i.e., erosion, sedimentation) and tectonic setting are currently poorly understood. In this study we analyze to what degree tectono-geomorphic setting and/or climatic characteristics control erosion rates in the region. We extract morphometric characteristics of the rift flanks and the plateau surface from SRTM 90-m resolution digital elevation data. We rely on calibrated, satellite-derived Tropical Rainfall Measurement Mission (TRMM 2B31) rainfall to characterize the different climatic compartments throughout the study region. We calculate specific stream power amounts using integrated rainfall as discharge amounts. Next, we analyze the relation between cosmogenic radionuclide (CRN) basin-wide erosion rates and climatic and geomorphic parameters. We determined erosion rates from twenty-six river sand samples acquired from along the flanks of the Elgeyo Escarpment (northern section of western rift flank), the Nguruman Escarpment (southern section of western flank), the Tirr Tirr Plateau (north), the Kapute Plains, and the Suguta Valley. Catchment-wide erosion rates range from 0.001 to 0.1 mm/y across the different climatic compartments. Comparisons to catchment climate and topographic characteristics suggest that more than 60% of variation in erosion can be explain by specific stream power amounts using rainfall as discharge component. The catchment-averaged normalized channel steepness index, which doesn't take into account variations in precipitation, explains only 42% of the variation in erosion rates. These observations demonstrate that the strong spatial variations in erosion rates are largely controlled by both catchment morphology and climatic gradients. In

  13. Videopanorama Frame Rate Requirements Derived from Visual Discrimination of Deceleration During Simulated Aircraft Landing

    NASA Technical Reports Server (NTRS)

    Furnstenau, Norbert; Ellis, Stephen R.

    2015-01-01

    In order to determine the required visual frame rate (FR) for minimizing prediction errors with out-the-window video displays at remote/virtual airport towers, thirteen active air traffic controllers viewed high dynamic fidelity simulations of landing aircraft and decided whether aircraft would stop as if to be able to make a turnoff or whether a runway excursion would be expected. The viewing conditions and simulation dynamics replicated visual rates and environments of transport aircraft landing at small commercial airports. The required frame rate was estimated using Bayes inference on prediction errors by linear FRextrapolation of event probabilities conditional on predictions (stop, no-stop). Furthermore estimates were obtained from exponential model fits to the parametric and non-parametric perceptual discriminabilities d' and A (average area under ROC-curves) as dependent on FR. Decision errors are biased towards preference of overshoot and appear due to illusionary increase in speed at low frames rates. Both Bayes and A - extrapolations yield a framerate requirement of 35 < FRmin < 40 Hz. When comparing with published results [12] on shooter game scores the model based d'(FR)-extrapolation exhibits the best agreement and indicates even higher FRmin > 40 Hz for minimizing decision errors. Definitive recommendations require further experiments with FR > 30 Hz.

  14. Disparities between in situ and optically derived carbon biomass and growth rates of the prymnesiophyte Phaeocystis globosa

    NASA Astrophysics Data System (ADS)

    Peperzak, L.; van der Woerd, H. J.; Timmermans, K. R.

    2015-03-01

    The oceans play a pivotal role in the global carbon cycle. It is not practical to measure the global daily production of organic carbon, the product of phytoplankton standing stock and its growth rate using discrete oceanographic methods. Instead, optical proxies from Earth-orbiting satellites must be used. To test the accuracy of optically derived proxies of phytoplankton physiology and growth rate, hyperspectral reflectance data from the wax and wane of a Phaeocystis bloom in laboratory mesocosms were compared with standard ex situ data. Chlorophyll biomass could be estimated accurately from reflectance using specific chlorophyll absorption algorithms. However, the conversion of chlorophyll (Chl) to carbon (C) was obscured by the non-linear increase in C : Chl under nutrient-limited growth. Although C : Chl was inversely correlated (r2 = 0.88) with the in situ fluorometric growth rate indicator Fv / Fm (Photosystem II quantum efficiency), none of them was linearly correlated to growth rate, constraining the accurate calculation of Phaeocystis growth or production rates. Unfortunately, the optical proxy ϕph (quantum efficiency of fluorescence: the ratio of the number of fluoresced photons to the number of photons absorbed by the phytoplankton) did not show any correlation with Phaeocystis growth rate, and therefore it is concluded that ϕph cannot be applied in the remotely sensed measurement of this species' carbon production rate.

  15. Right ventricular involvement in anterior myocardial infarction: a tissue Doppler-derived strain and strain rate study

    PubMed Central

    Sonmez, Osman; Kayrak, Mehmet; Altunbas, Gokhan; Abdulhalikov, Turyan; Alihanoglu, Yusuf; Bacaksiz, Ahmet; Ozdemir, Kurtulus; Gok, Hasan

    2013-01-01

    OBJECTIVE: Strain and strain rate imaging is currently the most popular echocardiographic technique that reveals subclinical myocardial damage. There are currently no available data on this imaging method with regard to assessing right ventricular involvement in anterior myocardial infarction. Therefore, we aimed to evaluate right ventricular regional functions using a derived strain and strain rate imaging tissue Doppler method in patients who were successfully treated for their first anterior myocardial infarction. METHODS: The patient group was composed of 44 patients who had experienced their first anterior myocardial infarction and had undergone successful percutaneous coronary intervention. Twenty patients were selected for the control group. The right ventricular myocardial samplings were performed in three regions: the basal, mid, and apical segments of the lateral wall. The individual myocardial velocity, strain, and strain rate values of each basal, mid, and apical segment were obtained. RESULTS: The right ventricular myocardial velocities of the patient group were significantly decreased with respect to all three velocities in the control group. The strain and strain rate values of the right mid and apical ventricular segments in the patient group were significantly lower than those of the control group (excluding the right ventricular basal strain and strain rate). In addition, changes in the right ventricular mean strain and strain rate values were significant. CONCLUSION: Right ventricular involvement following anterior myocardial infarction can be assessed using tissue Doppler based strain and strain rate PMID:24141839

  16. Central and eastern Anatolian crustal deformation rate and velocity fields derived from GPS and earthquake data

    NASA Astrophysics Data System (ADS)

    Simão, N. M.; Nalbant, S. S.; Sunbul, F.; Komec Mutlu, A.

    2016-01-01

    We present a new strain-rate and associated kinematic model for the eastern and central parts of Turkey. In the east, a quasi N-S compressional tectonic regime dominates the deformation field and is partitioned through the two major structural elements of the region, which are the conjugate dextral strike-slip North Anatolian Fault Zone (NAFZ) and the sinistral strike slip East Anatolian Fault Zone (EAFZ). The observed surface deformation is similar to that inferred by anisotropy studies which sampled the region of the mantle closer to the crust (i.e. the lithospheric mantle and the Moho), and is dependent on the presence or absence of a lithospheric mantle, and of the level of coupling between it and the overlaying crust. The areas of the central and eastern parts of Turkey which are deforming at elevated rates are situated above areas with strong gradients in crustal thickness. This seems to indicate that these transition zones, situated between thinner and thicker crusts, promote more deformation at the surface. The regions that reveal elevated strain-rate values are 1) the Elaziğ-Bingol segment of the EAFZ, 2) the region around the Karlıova triple-junction including the Yedisu segment and the Varto fault, 3) the section of the NAFZ that extends from the Erzincan province up to the NAFZ-Ezinepazarı fault junction, and 4) sections of the Tuz Gölü Fault Zone. Other regions like the Adana basin, a significant part of the Central Anatolian Fault Zone (CAFZ), the Aksaray and the Ankara provinces, are deforming at smaller but still considerable rates and therefore should be considered as areas well capable of producing damaging earthquakes (between M6 and 7). This study also reveals that the central part of Turkey is moving at a faster rate towards the west than the eastern part Turkey, and that the wedge region between the NAFZ and the EAFZ accounts for the majority of the counter clockwise rotation between the eastern and the central parts of Turkey. This

  17. Oxygen consumption rates in subseafloor basaltic crust derived from a reaction transport model.

    PubMed

    Orcutt, Beth N; Wheat, C Geoffrey; Rouxel, Olivier; Hulme, Samuel; Edwards, Katrina J; Bach, Wolfgang

    2013-01-01

    Oceanic crust is the largest potential habitat for life on Earth and may contain a significant fraction of Earth's total microbial biomass; yet, empirical analysis of reaction rates in basaltic crust is lacking. Here we report the first assessment of oxygen consumption in young (~8 Ma) and cool (<25 °C) basaltic crust, which we calculate from modelling dissolved oxygen and strontium pore water gradients in basal sediments collected during Integrated Ocean Drilling Program Expedition 336 to 'North Pond' on the western flank of the Mid-Atlantic Ridge. Dissolved oxygen is completely consumed within the upper to middle section of the sediment column, with an increase in concentration towards the sediment-basement interface, indicating an upward supply from oxic fluids circulating within the crust. A parametric reaction transport model of oxygen behaviour in upper basement suggests oxygen consumption rates of 1 nmol  cm(-3)ROCK d(-1) or less in young and cool basaltic crust.

  18. Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol.

    PubMed

    Polburee, Pirapan; Yongmanitchai, Wichien; Lertwattanasakul, Noppon; Ohashi, Takao; Fujiyama, Kazuhito; Limtong, Savitree

    2015-12-01

    This study attempted to identify oleaginous yeasts and selected the strain that accumulated the largest quantity of lipid for lipid production from glycerol. Two-step screening of 387 yeast strains revealed 23 oleaginous strains that accumulated quantities of lipid higher than 20 % of their biomass when cultivated in glycerol. These strains were identified to be four ascomycetous yeast species i.e. Candida silvae, Kodamaea ohmeri, Meyerozyma caribbica, and Pichia manshurica, and five basidiomycetous yeast species i.e. Cryptococcus cf. podzolicus, Cryptococcus laurentii, Rhodosporidium fluviale, Rhodotorula taiwanensis, and Sporidiobolus ruineniae. Rhodosporidium fluviale DMKU-RK253 accumulated the highest quantity of lipid equal to 65.2 % of its biomass (3.9 g L(-1) lipid and 6.0 g L(-1) biomass) by shaking flask cultivation in crude glycerol. The main fatty acids in the accumulated lipid of this strain consisted of oleic acid, linoleic acid, and palmitic acid. Therefore, R. fluviale DMKU-RK253 has potential for producing lipid for biodiesel manufacturing using crude glycerol as a feedstock.

  19. Asynchronous RTK precise DGNSS positioning method for deriving a low-latency high-rate output

    NASA Astrophysics Data System (ADS)

    Liang, Zhang; Hanfeng, Lv; Dingjie, Wang; Yanqing, Hou; Jie, Wu

    2015-07-01

    Low-latency high-rate (1 Hz) precise real-time kinematic (RTK) can be applied in high-speed scenarios such as aircraft automatic landing, precise agriculture and intelligent vehicle. The classic synchronous RTK (SRTK) precise differential GNSS (DGNSS) positioning technology, however, is not able to obtain a low-latency high-rate output for the rover receiver because of long data link transmission time delays (DLTTD) from the reference receiver. To overcome the long DLTTD, this paper proposes an asynchronous real-time kinematic (ARTK) method using asynchronous observations from two receivers. The asynchronous observation model (AOM) is developed based on undifferenced carrier phase observation equations of the two receivers at different epochs with short baseline. The ephemeris error and atmosphere delay are the possible main error sources on positioning accuracy in this model, and they are analyzed theoretically. In a short DLTTD and during a period of quiet ionosphere activity, the main error sources decreasing positioning accuracy are satellite orbital errors: the "inverted ephemeris error" and the integration of satellite velocity error which increase linearly along with DLTTD. The cycle slip of asynchronous double-differencing carrier phase is detected by TurboEdit method and repaired by the additional ambiguity parameter method. The AOM can deal with synchronous observation model (SOM) and achieve precise positioning solution with synchronous observations as well, since the SOM is only a specific case of AOM. The proposed method not only can reduce the cost of data collection and transmission, but can also support the mobile phone network data link transfer mode for the data of the reference receiver. This method can avoid data synchronizing process besides ambiguity initialization step, which is very convenient for real-time navigation of vehicles. The static and kinematic experiment results show that this method achieves 20 Hz or even higher rate output in

  20. Automatic stress-relieving music recommendation system based on photoplethysmography-derived heart rate variability analysis.

    PubMed

    Shin, Il-Hyung; Cha, Jaepyeong; Cheon, Gyeong Woo; Lee, Choonghee; Lee, Seung Yup; Yoon, Hyung-Jin; Kim, Hee Chan

    2014-01-01

    This paper presents an automatic stress-relieving music recommendation system (ASMRS) for individual music listeners. The ASMRS uses a portable, wireless photoplethysmography module with a finger-type sensor, and a program that translates heartbeat signals from the sensor to the stress index. The sympathovagal balance index (SVI) was calculated from heart rate variability to assess the user's stress levels while listening to music. Twenty-two healthy volunteers participated in the experiment. The results have shown that the participants' SVI values are highly correlated with their prespecified music preferences. The sensitivity and specificity of the favorable music classification also improved as the number of music repetitions increased to 20 times. Based on the SVI values, the system automatically recommends favorable music lists to relieve stress for individuals.

  1. On rates and mechanisms of OH and O3 reactions with isoprene-derived hydroxy nitrates.

    PubMed

    Lee, Lance; Teng, Alex P; Wennberg, Paul O; Crounse, John D; Cohen, Ronald C

    2014-03-06

    Eight distinct hydroxy nitrates are stable products of the first step in the atmospheric oxidation of isoprene by OH. The subsequent chemical fate of these molecules affects global and regional production of ozone and aerosol as well as the location of nitrogen deposition. We synthesized and purified 3 of the 8 isoprene hydroxy nitrate isomers: (E/Z)-2-methyl-4-nitrooxybut-2-ene-1-ol and 3-methyl-2-nitrooxybut-3-ene-1-ol. Oxidation of these molecules by OH and ozone was studied using both chemical ionization mass spectrometry and thermo-dissociation laser induced fluorescence. The OH reaction rate constants at 300 K measured relative to propene at 745 Torr are (1.1 ± 0.2) × 10(-10) cm(3) molecule(-1) s(-1) for both the E and Z isomers and (4.2 ± 0.7) × 10(-11) cm(3) molecule(-1) s(-1) for the third isomer. The ozone reaction rate constants for (E/Z)-2-methyl-4-nitrooxybut-2-ene-1-ol are (2.7 ± 0.5) × 10(-17) and (2.9 ± 0.5) × 10(-17) cm(3) molecule(-1) s(-1), respectively. 3-Methyl-2-nitrooxybut-3-ene-1-ol reacts with ozone very slowly, within the range of (2.5-5) × 10(-19) cm(3) molecule(-1) s(-1). Reaction pathways, product yields, and implications for atmospheric chemistry are discussed. A condensed mechanism suitable for use in atmospheric chemistry models is presented.

  2. Accumulate Repeat Accumulate Coded Modulation

    NASA Technical Reports Server (NTRS)

    Abbasfar, Aliazam; Divsalar, Dariush; Yao, Kung

    2004-01-01

    In this paper we propose an innovative coded modulation scheme called 'Accumulate Repeat Accumulate Coded Modulation' (ARA coded modulation). This class of codes can be viewed as serial turbo-like codes, or as a subclass of Low Density Parity Check (LDPC) codes that are combined with high level modulation. Thus at the decoder belief propagation can be used for iterative decoding of ARA coded modulation on a graph, provided a demapper transforms the received in-phase and quadrature samples to reliability of the bits.

  3. The new conversion model MODERN to derive erosion rates from inventories of fallout radionuclides

    NASA Astrophysics Data System (ADS)

    Arata, Laura; Meusburger, Katrin; Frenkel, Elena; A'Campo-Neuen, Annette; Iurian, Andra-Rada; Ketterer, Michael E.; Mabit, Lionel; Alewell, Christine

    2016-04-01

    The measurement of fallout radionuclides (FRNs) has become one of the most commonly used methods to quantify soil erosion and depositional processes. FRNs include anthropogenic radionuclides (e.g. 137Cs, 239+240Pu) released into the atmosphere during nuclear bomb tests and power plant accidents (e.g Chernobyl, Fukushima-Daiichi), as well as natural radiotracers such as 210Pbex and 7Be. FRNs reach the land surface by dry and wet fallouts from the atmosphere. Once deposited, FRNs are tightly adsorbed by fine soil particles and their subsequent redistribution is mostly associated with soil erosion processes. FRNs methods are based on a qualitative comparison: the inventory (total radionuclide activity per unit area) at a given sampling site is compared to that of a so called reference site. The conversion of FRN inventories into soil erosion and deposition rates is done with a variety of models, which suitability is dependent on the selected FRN, soil cultivation (ploughed or unploughed) and movement (erosion or deposition). The authors propose a new conversion model, which can be easily and comprehensively used for different FRNs, land uses and soil redistribution processes. This new model i.e. MODERN (MOdelling Deposition and Erosion rates with RadioNuclides) considers the precise depth distribution of a given FRN at a reference site, and allows adapting it for any specific site conditions. MODERN adaptability and performance has been tested on two published case studies: (i) a 137Cs study in an alpine and unploughed area in the Aosta valley (Italy) and (ii) a 210Pbex study on a ploughed area located in Romania. The results show a good agreement and a significant correlation (r= 0.91, p<0.0001) between the results of MODERN and the published models currently used by the FRN scientific community (i.e. the Profile Distribution Model and the Mass Balance Model). The open access code and the cost free accessibility of MODERN will ensure the promotion of a wider

  4. Pregnancy rate following transfer of in vitro produced lamb derived embryos in two embryonic stages.

    PubMed

    Shirazi, A; Shams-Esfandabadi, N; Ahmadi, E; Jadidi, M; Heidari, B

    2008-03-15

    Ovine embryos were produced by maturation, fertilization and in vitro culture (IVM/IVF/IVC) of oocytes collected from slaughtered prepubertal ewes. At 24 h post IVM, oocytes were fertilized with fresh semen collected from Lori-Bakhtiari breed at a concentration of 1.0 x l0(6) sperm mL(-1). The presumptive ova/embryos were transferred into the embryo culture medium at 22-24 h post IVF. Following 4 to 7 day in culture, embryos (at morula and blastocyst stage, respectively) were transferred surgically to the uterine horn of synchronized recipients. Pregnancy was diagnosed at day 30 by hormonal assay and at days 55 and 140 of gestation by ultrasonography and pregnancies were allowed to go to term. A total of nine ewes received 27 embryos (3 embryos/ewe). Five ewes received 15 embryos at morula stage and four ewes received 12 embryos at blastocyst stage. From those received morula stage embryos one was pregnant on day 30 (20%), though no pregnancy was diagnosed on each of days 55 and 140. While from those received blastocyst stage embryos, three ewes were pregnant on day 30 (75%) and two ewes (50%) remained pregnant on each of days 55 and 140. In conclusion, day 4 IVM-IVF morula stage embryos had a lower survival rate than did day 7 IVM-IVF blastocysts embryos, following transfer to the synchronized recipient ewes.

  5. Cryosurvival and pregnancy rates after exposure of IVF-derived Bos indicus embryos to forskolin before vitrification.

    PubMed

    Sanches, B V; Marinho, L S R; Filho, B D O; Pontes, J H F; Basso, A C; Meirinhos, M L G; Silva-Santos, K C; Ferreira, C R; Seneda, M M

    2013-09-01

    In vitro-produced (IVP) bovine embryos are more sensitive to cryopreservation than their in vivo counterparts due to their higher lipid concentrations, whereas Bos indicus IVP embryos are even more sensitive than Bos taurus IVP embryos. To examine the effects of a lipolytic agent, before vitrification of Bos indicus IVP embryos, on embryo survival, viability, and pregnancy rates, two experiments were conducted. In experiment 1, Bos indicus (Nelore) embryos were produced from abattoir-derived ovaries and allocated into two groups. In the treatment group, 10 μM of forskolin was added to the in vitro culture medium on Day 5 and incubated for 48 hours. On Day 7 of culture, IVP-expanded blastocysts from both the control (n = 101) and treatment (n = 112) groups were vitrified with ethylene glycol and DMSO via the Cryotop procedure. Although there was no significant difference between the rates of blastocoel reexpansion and hatching of the embryos exposed to forskolin (87.5% and 70.5%, respectively) compared with the control embryos (79.2% and 63.3%, respectively), the numerically superior rates of the embryos exposed to forskolin led to another experiment. In experiment 2, blastocysts produced from the ovum pick up were exposed or not exposed to the lipolytic agent and vitrified as in experiment 1. Embryos treated with forskolin had higher pregnancy rates than the control group (48.8% vs. 18.5%). In view of these results, 1908 Bos indicus embryos were produced from ovum pick up, exposed to the lipolytic agent, and blastocysts were transferred to recipients, and the pregnancy rates of the embryos of various breeds were compared. The mean pregnancy rate obtained was 43.2%. All data were analyzed by chi-square or by binary logistic regression (P ≤ 0.05). In conclusion, treatment with forskolin before vitrification improved cryotolerance of Bos indicus IVP embryos, resulting in good post-transfer pregnancy rates.

  6. Snow accumulation variability derived from radar and firn core data along a 600 km transect in Adelie Land, East Antarctic plateau

    NASA Astrophysics Data System (ADS)

    Verfaillie, D.; Fily, M.; Le Meur, E.; Magand, O.; Jourdain, B.; Arnaud, L.; Favier, V.

    2012-11-01

    The mass balance of ice sheets is an intensively studied topic in the context of global change and sea-level rise. However - particularly in Antarctica - obtaining mass balance estimates remains difficult due to various logistical problems. In the framework of the TASTE-IDEA (Trans-Antarctic Scientific Traverses Expeditions - Ice Divide of East Antarctica) program, an International Polar Year project, continuous ground penetrating radar (GPR) measurements were carried out during a traverse in Adelie Land (East Antarctica) during the 2008-2009 austral summer between the Italian-French Dome C (DC) polar plateau site and French Dumont D'Urville (DdU) coastal station. The aim of this study was to process and interpret GPR data in terms of snow accumulation, to analyse its spatial and temporal variability and compare it with historical data and modelling. The focus was on the last 300 yr, from the pre-industrial period to recent times. Beta-radioactivity counting and gamma spectrometry were applied to cores at the LGGE laboratory, providing a depth-age calibration for radar measurements. Over the 600 km of usable GPR data, depth and snow accumulation were determined with the help of three distinct layers visible on the radargrams (≈ 1730, 1799 and 1941 AD). Preliminary results reveal a gradual increase in accumulation towards the coast (from ≈ 3 cm w.e. a-1 at Dome C to ≈ 17 cm w.e. a-1 at the end of the transect) and previously undocumented undulating structures between 300 and 600 km from DC. Results agree fairly well with data from previous studies and modelling. Drawing final conclusions on temporal variations is difficult because of the margin of error introduced by density estimation. This study should have various applications, including model validation.

  7. Snow accumulation variability in Adelie Land (East Antarctica) derived from radar and firn core data. A 600 km transect from Dome C

    NASA Astrophysics Data System (ADS)

    Verfaillie, D.; Fily, M.; Le Meur, E.; Magand, O.; Jourdain, B.; Arnaud, L.; Favier, V.

    2012-07-01

    Polar ice sheets mass balance is a timely topic intensively studied in the context of global change and sea-level rise. However, obtaining mass balance estimates in Antarctica in particular, remains difficult due to various logistical problems. In the framework of the TASTE-IDEA program, labeled as an International Polar Year project, continuous Ground Penetrating Radar (GPR) measurements were carried out during a traverse realised in Adelie Land (East Antarctica) during the 2008-2009 austral summer between the Italo-French Dome C (DC) polar plateau site and French Dumont D'Urville (DdU) coastal station. The aim of this study was to process and interpret GPR data in terms of snow accumulation, to analyse its spatial and temporal variability along the DC-DdU traverse and compare it with historical data and modeling. The emphasis has been put on the last 300 yr, from the pre-industrial to recent time period. Beta-radioactivity counting and gamma spectrometry were studied in cores at LGGE laboratory, providing a depth-age calibration for radar measurements. Over the 600 km of usable GPR data, depth and snow accumulation were determined with the help of three distinct layers visible on the radargrams (≈1730, 1799 and 1941 AD). Preliminary results reveal a gradual accumulation increase towards the coast and the occurrence of previously undocumented undulating structures between 300 and 600 km from DC. Results agree fairly well with data from previous studies and modeling. Concluding on temporal variations is difficult because of the margin of error introduced by density estimation. This study should have various applications such as for model validation.

  8. Radar-derived asteroid shapes point to a 'zone of stability' for topography slopes and surface erosion rates

    NASA Astrophysics Data System (ADS)

    Richardson, J.; Graves, K.; Bowling, T.

    2014-07-01

    Previous studies of the combined effects of asteroid shape, spin, and self-gravity have focused primarily upon the failure limits for bodies with a variety of standard shapes, friction, and cohesion values [1,2,3]. In this study, we look in the opposite direction and utilize 22 asteroid shape-models derived from radar inversion [4] and 7 small body shape-models derived from spacecraft observations [5] to investigate the region in shape/spin space [1,2] wherein self-gravity and rotation combine to produce a stable minimum state with respect to surface potential differences, dynamic topography, slope magnitudes, and erosion rates. This erosional minimum state is self-correcting, such that changes in the body's rotation rate, either up or down, will increase slope magnitudes across the body, thereby driving up erosion rates non-linearly until the body has once again reached a stable, minimized surface state [5]. We investigated this phenomenon in a systematic fashion using a series of synthesized, increasingly prolate spheroid shape models. Adjusting the rotation rate of each synthetic shape to minimize surface potential differences, dynamic topography, and slope magnitudes results in the magenta curve of the figure (right side), defining the zone of maximum surface stability (MSS). This MSS zone is invariant both with respect to body size (gravitational potential and rotational potential scale together with radius), and density when the scaled-spin of [2] is used. Within our sample of observationally derived small-body shape models, slow rotators (Group A: blue points), that are not in the maximum surface stability (MSS) zone and where gravity dominates the slopes, will generally experience moderate erosion rates (left plot) and will tend to move up and to the right in shape/spin space as the body evolves (right plot). Fast rotators (Group C: red points), that are not in the MSS zone and where spin dominates the slopes, will generally experience high erosion rates

  9. Honeysuckle-derived hierarchical porous nitrogen, sulfur, dual-doped carbon for ultra-high rate lithium ion battery anodes

    NASA Astrophysics Data System (ADS)

    Ou, Junke; Yang, Lin; Zhang, Zhen; Xi, Xianghui

    2016-11-01

    Nowadays, developing functional carbon materials from cheap natural materials is a highly compelling topic. Different from most explored biomass, honeysuckle is inherently rich in nitrogen and sulfur heteroatoms, and it has many advantages for production on a large scale. Here, hierarchical porous carbon (HPC), derived from waste honeysuckle via an environmentally friendly and economically viable method, has been reported as an anode for rechargeable lithium ion batteries. The as-fabricated HPC exhibits favorable features for electrochemical energy storage performance such as high specific surface area (830 m2 g-1), hierarchical three-dimensional (3D) pore network and heteroatoms (N and S) doping effects. HPC, when evaluated as an anode material for lithium ion batteries, shows superior cycling stability (maintaining a reversible capacity of 1215 mAh g-1 at the current density of 100 mA g-1 after 100 cycles) and excellent rate capability (370 mAh g-1 at the current density of 20 A g-1). Furthermore, owing to the appropriate heteroatoms doping, a high initial coulombic efficiency of 64.7% can be achieved. A widespread comparison with the literature also showed that the honeysuckle derived porous carbon was one of the most promising carbon-based anodes for high-rate lithium ion batteries.

  10. Characterization of the l-alanine exporter AlaE of Escherichia coli and its potential role in protecting cells from a toxic-level accumulation of l-alanine and its derivatives.

    PubMed

    Kim, Seryoung; Ihara, Kohei; Katsube, Satoshi; Hori, Hatsuhiro; Ando, Tasuke; Isogai, Emiko; Yoneyama, Hiroshi

    2015-08-01

    We previously reported that the alaE gene of Escherichia coli encodes the l-alanine exporter AlaE. The objective of this study was to elucidate the mechanism of the AlaE exporter. The minimum inhibitory concentration of l-alanine and l-alanyl-l-alanine in alaE-deficient l-alanine-nonmetabolizing cells MLA301ΔalaE was 4- and >4000-fold lower, respectively, than in the alaE-positive parent cells MLA301, suggesting that AlaE functions as an efflux pump to avoid a toxic-level accumulation of intracellular l-alanine and its derivatives. Furthermore, the growth of the alaE-deficient mutant derived from the l-alanine-metabolizing strain was strongly inhibited in the presence of a physiological level of l-alanyl-l-alanine. Intact MLA301ΔalaE and MLA301ΔalaE/pAlaE cells producing plasmid-borne AlaE, accumulated approximately 200% and 50%, respectively, of the [(3) H]l-alanine detected in MLA301 cells, suggesting that AlaE exports l-alanine. When 200 mmol/L l-alanine-loaded inverted membrane vesicles prepared from MLA301ΔalaE/pAlaE were placed in a solution containing 200 mmol/L or 0.34 μmol/L l-alanine, energy-dependent [(3) H]l-alanine accumulation occurred under either condition. This energy-dependent uphill accumulation of [(3) H]l-alanine was strongly inhibited in the presence of carbonyl cyanide m-chlorophenylhydrazone but not by dicyclohexylcarbodiimide, suggesting that the AlaE-mediated l-alanine extrusion was driven by proton motive force. Based on these results, physiological roles of the l-alanine exporter are discussed.

  11. Cellular change and callose accumulation in zygotic embryos of Eleutherococcus senticosus caused by plasmolyzing pretreatment result in high frequency of single-cell-derived somatic embryogenesis.

    PubMed

    Ling You, Xiang; Seon Yi, Jae; Eui Choi, Yong

    2006-05-01

    Eleutherococcus senticosus zygotic embryos were pretreated with 1.0 M mannitol or sucrose for 3-24 h. This pretreatment resulted in a high frequency of somatic-embryo formation on hormone-free medium. All the somatic embryos developed directly and independently from single epidermal cells on the surface of zygotic embryos after plasmolyzing pretreatment. Scanning electron microscopic observation revealed that the epidermal cells of hypocotyls rapidly became irregular and showed a random orientation before somatic-embryo development commenced. At the same time, the epidermal cells in the untreated control remained regular. Callose concentration determined by fluorometric analysis increased sharply in E. senticosus zygotic embryos after plasmolyzing pretreatment but remained low in the untreated control. Aniline blue fluorescent staining of callose showed that the plasmolyzing pretreatment of zygotic embryos resulted in heavy accumulation of callose between the plasma membrane and cell walls. On the basis of these results, we suggest that plasmolyzing pretreatment of zygotic embryos induces the accumulation of callose, and the interruption of cell-to-cell communication imposed by this might stimulate the reprogramming of epidermal cells into embryogenically competent cells and finally induce somatic-embryo development from single cells.

  12. Investigating the past and recent δ18O-accumulation relationship seen in Greenland ice cores

    NASA Astrophysics Data System (ADS)

    Buchardt, S. L.; Clausen, H. B.; Vinther, B. M.; Dahl-Jensen, D.

    2012-08-01

    Decadal means of δ18O and accumulation rates from 52 ice core sites in Greenland are presented. The accumulation rates are derived from annual layers determined in the δ18O curve. Investigation of the δ18O-accumulation relationship across the ice divide reveals a significant Foehn effect with anticorrelation of δ18O and accumulation on the lee side of the divide in Southern Greenland, while no effect is seen in Central Greenland. Furthermore, the sensitivity of accumulation rate to changes in temperature is found to be smaller in Northern Greenland than in the central and southern parts. Four sites in the data set contain sufficient recent data that the period of observed temperature rise from the 1990's and onwards can be investigated. All four sites are located close to the ice divide in Northern Greenland and while three sites show increased temperatures, none show evidence of increased accumulation.

  13. Transgenic tobacco and Arabidopsis plants expressing the two multifunctional sorghum cytochrome P450 enzymes, CYP79A1 and CYP71E1, are cyanogenic and accumulate metabolites derived from intermediates in Dhurrin biosynthesis.

    PubMed

    Bak, S; Olsen, C E; Halkier, B A; Møller, B L

    2000-08-01

    Novel cyanogenic plants have been generated by the simultaneous expression of the two multifunctional sorghum (Sorghum bicolor [L.] Moench) cytochrome P450 enzymes CYP79A1 and CYP71E1 in tobacco (Nicotiana tabacum cv Xanthi) and Arabidopsis under the regulation of the constitutive 35S promoter. CYP79A1 and CYP71E1 catalyze the conversion of the parent amino acid tyrosine to p-hydroxymandelonitrile, the aglycone of the cyanogenic glucoside dhurrin. CYP79A1 catalyzes the conversion of tyrosine to p-hydroxyphenylacetaldoxime and CYP71E1, the subsequent conversion to p-hydroxymandelonitrile. p-Hydroxymandelonitrile is labile and dissociates into p-hydroxybenzaldehyde and hydrogen cyanide, the same products released from dhurrin upon cell disruption as a result of pest or herbivore attack. In transgenic plants expressing CYP79A1 as well as CYP71E1, the activity of CYP79A1 is higher than that of CYP71E1, resulting in the accumulation of several p-hydroxyphenylacetaldoxime-derived products in the addition to those derived from p-hydroxymandelonitrile. Transgenic tobacco and Arabidopsis plants expressing only CYP79A1 accumulate the same p-hydroxyphenylacetaldoxime-derived products as transgenic plants expressing both sorghum cytochrome P450 enzymes. In addition, the transgenic CYP79A1 Arabidopsis plants accumulate large amounts of p-hydroxybenzylglucosinolate. In transgenic Arabidopsis expressing CYP71E1, this enzyme and the enzymes of the pre-existing glucosinolate pathway compete for the p-hydroxyphenylacetaldoxime as substrate, resulting in the formation of small amounts of p-hydroxybenzylglucosinolate. Cyanogenic glucosides are phytoanticipins, and the present study demonstrates the feasibility of expressing cyanogenic compounds in new plant species by gene transfer technology to improve pest and disease resistance.

  14. Heat accumulation during pulsed laser materials processing.

    PubMed

    Weber, Rudolf; Graf, Thomas; Berger, Peter; Onuseit, Volkher; Wiedenmann, Margit; Freitag, Christian; Feuer, Anne

    2014-05-05

    Laser materials processing with ultra-short pulses allows very precise and high quality results with a minimum extent of the thermally affected zone. However, with increasing average laser power and repetition rates the so-called heat accumulation effect becomes a considerable issue. The following discussion presents a comprehensive analytical treatment of multi-pulse processing and reveals the basic mechanisms of heat accumulation and its consequence for the resulting processing quality. The theoretical findings can explain the experimental results achieved when drilling microholes in CrNi-steel and for cutting of CFRP. As a consequence of the presented considerations, an estimate for the maximum applicable average power for ultra-shorts pulsed laser materials processing for a given pulse repetition rate is derived.

  15. Stevia-derived compounds attenuate the toxic effects of ectopic lipid accumulation in the liver of obese mice: a transcriptomic and metabolomic study.

    PubMed

    Holvoet, Paul; Rull, Anna; García-Heredia, Anabel; López-Sanromà, Sílvia; Geeraert, Benjamine; Joven, Jorge; Camps, Jordi

    2015-03-01

    There is a close interaction between Type 2 Diabetes, obesity and liver disease. We have studied the effects of the two most abundant Stevia-derived steviol glycosides, stevioside and rebaudioside A, and their aglycol derivative steviol on liver steatosis and the hepatic effects of lipotoxicity using a mouse model of obesity and insulin resistance. We treated ob/ob and LDLR-double deficient mice with stevioside (10 mg⋅kg(-1)⋅day-1 p.o., n = 8), rebaudioside A (12 mg⋅kg(-1)⋅day-1 p.o., n = 8), or steviol (5 mg⋅kg(-1)⋅day(-1) p.o., n = 8). We determined their effects on liver steatosis and on the metabolic effects of lipotoxicity by histological analysis, and by combined gene-expression and metabolomic analyses. All compounds attenuated hepatic steatosis. This could be explained by improved glucose metabolism, fat catabolism, bile acid metabolism, and lipid storage and transport. We identified PPARs as important regulators and observed differences in effects on insulin resistance, inflammation and oxidative stress between Stevia-derived compounds. We conclude that Stevia-derived compounds reduce hepatic steatosis to a similar extent, despite differences in effects on glucose and lipid metabolism, and inflammation and oxidative stress. Thus our data show that liver toxicity can be reduced through several pathophysiological changes. Further identification of active metabolites and underlying mechanisms are warranted.

  16. In-situ Measurements of Ozone Production Rates and Comparisons to Model-derived Production Rates During the Houston, TX and Denver, CO DISCOVER-AQ Campaigns

    NASA Astrophysics Data System (ADS)

    Baier, B. C.; Brune, W. H.; Miller, D. O.; Lefer, B. L.

    2015-12-01

    Tropospheric ozone (O3) is a secondary pollutant that has harmful effects on human and plant life. The climate and urban emissions in Houston, TX and Denver, CO can be conducive for significant ozone production and thus, high ozone events. Tighter government strategies for ozone mitigation have been proposed, which involve reducing the current EPA eight-hour ozone standard from 75 ppb to 65-70 ppb. These strategies rely on the reduction of ozone precursors in order to decrease the ozone production rate, P(O3). The changes in the ozone concentration at a certain location are dependent upon P(O3), so decreasing P(O3) can decrease ozone levels provided that it has not been transported from other areas. Air quality models test reduction strategies before they are implemented, locate ozone sources, and predict ozone episodes. Traditionally, P(O3) has been calculated by models. However, large uncertainties in model emissions inventories, chemical mechanisms, and meteorology can reduce confidence in this approach. A new instrument, the Measurement of Ozone Production Sensor (MOPS) directly measures P(O3) and can provide an alternate approach to determining P(O3). An updated version of the Penn State MOPS (MOPSv2.0) was deployed to Houston, TX and Denver, CO as a part of NASA's DISCOVER-AQ field campaign in the summers of 2013 and 2014, respectively. We present MOPS directly-measured P(O3) rates from these areas, as well as comparisons to zero-dimensional and three-dimensional modeled P(O3) using the RACM2 and MCMv2.2 mechanisms. These comparisons demonstrate the potential of the MOPS to test and evaluate model-derived P(O3), to advance the understanding of model chemical mechanisms, and to improve predictions of high ozone events.

  17. Slicing up the San Francisco Bay Area: Block kinematics and fault slip rates from GPS-derived surface velocities

    USGS Publications Warehouse

    d'Alessio, M. A.; Johanson, I.A.; Burgmann, R.; Schmidt, D.A.; Murray, M.H.

    2005-01-01

    Observations of surface deformation allow us to determine the kinematics of faults in the San Francisco Bay Area. We present the Bay Area velocity unification (BA??VU??, "bay view"), a compilation of over 200 horizontal surface velocities computed from campaign-style and continuous Global Positioning System (GPS) observations from 1993 to 2003. We interpret this interseismic velocity field using a three-dimensional block model to determine the relative contributions of block motion, elastic strain accumulation, and shallow aseismic creep. The total relative motion between the Pacific plate and the rigid Sierra Nevada/Great Valley (SNGV) microplate is 37.9 ?? 0.6 mm yr-1 directed toward N30.4??W ?? 0.8?? at San Francisco (??2??). Fault slip rates from our preferred model are typically within the error bounds of geologic estimates but provide a better fit to geodetic data (notable right-lateral slip rates in mm yr-1: San Gregorio fault, 2.4 ?? 1.0; West Napa fault, 4.0 ?? 3.0; zone of faulting along the eastern margin of the Coast Range, 5.4 ?? 1.0; and Mount Diablo thrust, 3.9 ?? 1.0 of reverse slip and 4.0 ?? 0.2 of right-lateral strike slip). Slip on the northern Calaveras is partitioned between both the West Napa and Concord/ Green Valley fault systems. The total convergence across the Bay Area is negligible. Poles of rotation for Bay Area blocks progress systematically from the North America-Pacific to North America-SNGV poles. The resulting present-day relative motion cannot explain the strike of most Bay Area faults, but fault strike does loosely correlate with inferred plate motions at the time each fault initiated. Copyright 2005 by the American Geophysical Union.

  18. Compartmentation Analysis of Paraquat Fluxes in Maize Roots as a Means of Estimating the Rate of Vacuolar Accumulation and Translocation to Shoots.

    PubMed Central

    DiTomaso, J. M.; Hart, J. J.; Kochian, L. V.

    1993-01-01

    Efflux analysis conducted after five loading periods of various lengths (2, 6, 12, 18, or 24 h) was used to investigate uptake, compartmentation, and translocation of [14C]paraquat in maize (Zea mays L.) seedlings. The time course for net paraquat uptake (paraquat concentration in uptake solution = 25[mu]M) into maize roots was linear (56.7 nmol g-1 root fresh weight h-1) for 24 h. Estimates of changes in paraquat content in the vacuole, cytoplasm, and cell wall after 2-, 6-, 12-, 18-, and 24-h loading periods indicated that the cell wall saturated rapidly, whereas accumulation of paraquat into the vacuole increased linearly (12.4 nmol g-1 root fresh weight h-1) over 24 h. In contrast to vacuolar accumulation, cytoplasmic paraquat content appeared to approach saturation. The half-time for paraquat efflux from the cell wall (16.6 min [plus or minus] 1.2 SD) and cytoplasm (58.8 min [plus or minus] 8.9 SD remained relatively constant regardless of the length of the loading period, whereas the half-time for efflux from the vacuole was considerably longer and increased linearly with increased loading time (6.1-18.7 h). The time course for paraquat translocation to the shoot was linear within a 24-h exposure to radiolabeled herbicide, but translocation did not begin until 5 h after initiation of treatment. The experimental approach used in these experiments provides a valuable method for examining the movement of paraquat in maize seedlings. Results indicate that the herbicide slowly accumulates in the vacuole of root cells but is also translocated to the shoot. PMID:12231834

  19. Intrinsic (gas phase) thermodynamic stability of 2-adamantyl cation. Its bearing on the solvolysis rates of 2-adamantyl derivatives.

    PubMed

    Abboud, José-Luis M; Castaño, Obis; Dávalos, Juan Z; Jiménez, Pilar; Gomperts, Roberto; Müller, Paul; Roux, María Victoria

    2002-02-22

    The standard enthalpy of formation of gaseous 2-adamantyl chloride(2-Ad-Cl) was determined by calorimetric techniques. The standard Gibbs energy change for the chloride anion exchange between 1-adamantyl (1-Ad+) and 2-adamantyl (2-Ad+) cations in the gas phase was obtained by Fourier transform ion cyclotron resonance spectroscopy (FT ICR). Theoretical calculations at the G2(MP2) level were performed on these and other relevant species. This and data from the literature provided three highly consistent independent estimates of the relative stabilities of 2-Ad+ and 1-Ad+. This difference in gas-phase stability was compared to the differential structural effects on the rates of solvolysis of the corresponding chlorides and tosylates, and it was shown that the thermodynamic stability of the secondary cation is the leading factor determining the solvolytic reactivity of the precursors in the absence of solvent effects. Thus, under these conditions, the previously established linear free energy correlation between carbenium ion stability and solvolytic reactivity of bridgehead derivatives applies also to secondary derivatives.

  20. Estimation of Potassium Recirculation in Tomato Plants by Comparison of the Rates of Potassium and Calcium Accumulation in the Tops with Their Fluxes in the Xylem Stream

    PubMed Central

    Armstrong, Michael J.; Kirkby, Ernest A.

    1979-01-01

    A procedure to estimate the extent of K recirculation in plants is proposed. This is based on the ratio of the upward fluxes of K to Ca in the xylem sap from root to shoot with the ratio of K to Ca accumulation in plant tops. In a preliminary investigation the factors influencing the K to Ca ratio in the xylem sap were considered. Tomato plants were grown at three levels of K nutrition and harvested at different times during the 24-hour day period. It was shown that the K to Ca ratio in xylem sap changed dramatically depending on the time of sap collection after decapitation, the values falling from over 2 to less than unity over the 4-hour period of collection. Diurnal effects on exudation were less marked but also of significance. The level of K nutrition was of little importance. It is suggested that a representative xylem sap from tomato plants can best be obtained from samples taken between 15 and 60 minutes after decapitation. In a second experiment K recirculation was estimated. At nine harvesting stages over a 24-hour period the K to Ca ratio in the xylem sap was invariably higher than the K to Ca ratio of accumulation in the tops over the same period. From this information it was calculated that about 20% of the upward flux of K in the xylem stream resulted from recirculated K. PMID:16660872

  1. Using a detailed uncertainty analysis to adjust mapped rates of forest disturbance derived from Landsat time series data (Invited)

    NASA Astrophysics Data System (ADS)

    Cohen, W. B.; Yang, Z.; Stehman, S.; Huang, C.; Healey, S. P.

    2013-12-01

    Forest ecosystem process models require spatially and temporally detailed disturbance data to accurately predict fluxes of carbon or changes in biodiversity over time. A variety of new mapping algorithms using dense Landsat time series show great promise for providing disturbance characterizations at an annual time step. These algorithms provide unprecedented detail with respect to timing, magnitude, and duration of individual disturbance events, and causal agent. But all maps have error and disturbance maps in particular can have significant omission error because many disturbances are relatively subtle. Because disturbance, although ubiquitous, can be a relatively rare event spatially in any given year, omission errors can have a great impact on mapped rates. Using a high quality reference disturbance dataset, it is possible to not only characterize map errors but also to adjust mapped disturbance rates to provide unbiased rate estimates with confidence intervals. We present results from a national-level disturbance mapping project (the North American Forest Dynamics project) based on the Vegetation Change Tracker (VCT) with annual Landsat time series and uncertainty analyses that consist of three basic components: response design, statistical design, and analyses. The response design describes the reference data collection, in terms of the tool used (TimeSync), a formal description of interpretations, and the approach for data collection. The statistical design defines the selection of plot samples to be interpreted, whether stratification is used, and the sample size. Analyses involve derivation of standard agreement matrices between the map and the reference data, and use of inclusion probabilities and post-stratification to adjust mapped disturbance rates. Because for NAFD we use annual time series, both mapped and adjusted rates are provided at an annual time step from ~1985-present. Preliminary evaluations indicate that VCT captures most of the higher

  2. Cell wall accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated Arabidopsis leaf epidermal cells.

    PubMed

    Akita, Kae; Kobayashi, Megumi; Sato, Mayuko; Kutsuna, Natsumaro; Ueda, Takashi; Toyooka, Kiminori; Nagata, Noriko; Hasezawa, Seiichiro; Higaki, Takumi

    2017-01-01

    In most dicotyledonous plants, leaf epidermal pavement cells develop jigsaw puzzle-like shapes during cell expansion. The rapid growth and complicated cell shape of pavement cells is suggested to be achieved by targeted exocytosis that is coordinated with cytoskeletal rearrangement to provide plasma membrane and/or cell wall materials for lobe development during their morphogenesis. Therefore, visualization of membrane trafficking in leaf pavement cells should contribute an understanding of the mechanism of plant cell morphogenesis. To reveal membrane trafficking in pavement cells, we observed monomeric red fluorescent protein-tagged rat sialyl transferases, which are markers of trans-Golgi cisternal membranes, in the leaf epidermis of Arabidopsis thaliana. Quantitative fluorescence imaging techniques and immunoelectron microscopic observations revealed that accumulation of the red fluorescent protein occurred mostly in the curved regions of pavement cell borders and guard cell ends during leaf expansion. Transmission electron microscopy observations revealed that apoplastic vesicular membrane structures called paramural bodies were more frequent beneath the curved cell wall regions of interdigitated pavement cells and guard cell ends in young leaf epidermis. In addition, pharmacological studies showed that perturbations in membrane trafficking resulted in simple cell shapes. These results suggested possible heterogeneity of the curved regions of plasma membranes, implying a relationship with pavement cell morphogenesis.

  3. An "Aufbau" Approach to Understanding How the King-Altman Method of Deriving Rate Equations for Enzyme-Catalyzed Reactions Works

    ERIC Educational Resources Information Center

    Sims, Paul A.

    2009-01-01

    The King-Altman method of deriving rate equations for enzymatic reactions is applied to the derivation of the Michaelis-Menten equation, along with an explanation for how (or why) the King-Altman method works in this case. The slightly more complicated cases of competitive inhibition and a two-substrate enzyme-catalyzed reaction are then treated…

  4. Bladder accumulated dose in image-guided high-dose-rate brachytherapy for locally advanced cervical cancer and its relation to urinary toxicity

    NASA Astrophysics Data System (ADS)

    Zakariaee, Roja; Hamarneh, Ghassan; Brown, Colin J.; Gaudet, Marc; Aquino-Parsons, Christina; Spadinger, Ingrid

    2016-12-01

    The purpose of this study was to estimate locally accumulated dose to the bladder in multi-fraction high-dose-date (HDR) image-guided intracavitary brachytherapy (IG-ICBT) for cervical cancer, and study the locally-accumulated dose parameters as predictors of late urinary toxicity. A retrospective study of 60 cervical cancer patients who received five HDR IG-ICBT sessions was performed. The bladder outer and inner surfaces were segmented for all sessions and a bladder-wall contour point-set was created in MATLAB. The bladder-wall point-sets for each patient were registered using a deformable point-set registration toolbox called coherent point drift (CPD), and the fraction doses were accumulated. Various dosimetric and volumetric parameters were calculated using the registered doses, including r{{\\text{D}}n \\text{c{{\\text{m}}\\text{3}}}} (minimum dose to the most exposed n-cm3 volume of bladder wall), r V n Gy (wall volume receiving at least m Gy), and r\\text{EQD}{{2}n \\text{c{{\\text{m}}\\text{3}}}} (minimum equivalent biologically weighted dose to the most exposed n-cm3 of bladder wall), where n  =  1/2/5/10 and m  =  3/5/10. Minimum dose to contiguous 1 and 2 cm3 hot-spot volumes was also calculated. The unregistered dose volume histogram (DVH)-summed equivalent of r{{\\text{D}}n \\text{c{{\\text{m}}3}}} and r\\text{EQD}{{2}n \\text{c{{\\text{m}}3}}} parameters (i.e. s{{\\text{D}}n \\text{c{{\\text{m}}\\text{3}}}} and s\\text{EQD}{{2}n \\text{c{{\\text{m}}3}}} ) were determined for comparison. Late urinary toxicity was assessed using the LENT-SOMA scale, with toxicity Grade 0-1 categorized as Controls and Grade 2-4 as Cases. A two-sample t-test was used to identify the differences between the means of Control and Case groups for all parameters. A binomial logistic regression was also performed between the registered dose parameters and toxicity grouping. Seventeen patients were in the Case and 43 patients in the Control group. Contiguous

  5. Carbon-Coated, Diatomite-Derived Nanosilicon as a High Rate Capable Li-ion Battery Anode

    NASA Astrophysics Data System (ADS)

    Campbell, Brennan; Ionescu, Robert; Tolchin, Maxwell; Ahmed, Kazi; Favors, Zachary; Bozhilov, Krassimir N.; Ozkan, Cengiz S.; Ozkan, Mihrimah

    2016-10-01

    Silicon is produced in a variety of ways as an ultra-high capacity lithium-ion battery (LIB) anode material. The traditional carbothermic reduction process required is expensive and energy-intensive; in this work, we use an efficient magnesiothermic reduction to convert the silica-based frustules within diatomaceous earth (diatomite, DE) to nanosilicon (nanoSi) for use as LIB anodes. Polyacrylic acid (PAA) was used as a binder for the DE-based nanoSi anodes for the first time, being attributed for the high silicon utilization under high current densities (up to 4C). The resulting nanoSi exhibited a high BET specific surface area of 162.6 cm2 g‑1, compared to a value of 7.3 cm2 g‑1 for the original DE. DE contains SiO2 architectures that make ideal bio-derived templates for nanoscaled silicon. The DE-based nanoSi anodes exhibit good cyclability, with a specific discharge capacity of 1102.1 mAh g‑1 after 50 cycles at a C-rate of C/5 (0.7 A gSi‑1) and high areal loading (2 mg cm‑2). This work also demonstrates the fist rate capability testing for a DE-based Si anode; C-rates of C/30 - 4C were tested. At 4C (14.3 A gSi‑1), the anode maintained a specific capacity of 654.3 mAh g‑1 – nearly 2x higher than graphite’s theoretical value (372 mAh g‑1).

  6. Carbon-Coated, Diatomite-Derived Nanosilicon as a High Rate Capable Li-ion Battery Anode

    PubMed Central

    Campbell, Brennan; Ionescu, Robert; Tolchin, Maxwell; Ahmed, Kazi; Favors, Zachary; Bozhilov, Krassimir N.; Ozkan, Cengiz S.; Ozkan, Mihrimah

    2016-01-01

    Silicon is produced in a variety of ways as an ultra-high capacity lithium-ion battery (LIB) anode material. The traditional carbothermic reduction process required is expensive and energy-intensive; in this work, we use an efficient magnesiothermic reduction to convert the silica-based frustules within diatomaceous earth (diatomite, DE) to nanosilicon (nanoSi) for use as LIB anodes. Polyacrylic acid (PAA) was used as a binder for the DE-based nanoSi anodes for the first time, being attributed for the high silicon utilization under high current densities (up to 4C). The resulting nanoSi exhibited a high BET specific surface area of 162.6 cm2 g−1, compared to a value of 7.3 cm2 g−1 for the original DE. DE contains SiO2 architectures that make ideal bio-derived templates for nanoscaled silicon. The DE-based nanoSi anodes exhibit good cyclability, with a specific discharge capacity of 1102.1 mAh g−1 after 50 cycles at a C-rate of C/5 (0.7 A gSi−1) and high areal loading (2 mg cm−2). This work also demonstrates the fist rate capability testing for a DE-based Si anode; C-rates of C/30 - 4C were tested. At 4C (14.3 A gSi−1), the anode maintained a specific capacity of 654.3 mAh g−1 – nearly 2x higher than graphite’s theoretical value (372 mAh g−1). PMID:27713474

  7. CNS invasion by CD14+/CD16+ peripheral blood-derived monocytes in HIV dementia: perivascular accumulation and reservoir of HIV infection.

    PubMed

    Fischer-Smith, T; Croul, S; Sverstiuk, A E; Capini, C; L'Heureux, D; Régulier, E G; Richardson, M W; Amini, S; Morgello, S; Khalili, K; Rappaport, J

    2001-12-01

    Increases in circulating CD14+/CD16+ monocytes have been associated with HIV dementia; trafficking of these cells into the CNS has been proposed to play an important role in the pathogenesis of HIV-induced neurological disorders. This model suggests that events outside the CNS leading to monocyte activation initiate the process leading to HIV dementia. To investigate the role of this activated monocyte subset in the pathogenesis of HIV dementia, we examined brain specimens from patients with HIV encephalopathy (HIVE), HIV without encephalopathy, and seronegative controls. An accumulation of perivascular macrophages was observed in HIVE. The majority of these cells identified in microglial nodules and in the perivascular infiltrate were CD14+/CD16+. P24 antigen colocalized with both CD14 and CD16 suggesting that the CD14+/CD16+ macrophage is a major reservoir of HIV-1 infection in CNS. Using CD45/LCA staining, the perivascular macrophage was distinguished from resident microglia. In addition to perivascular and nodular localizations, CD16 also stained ramified cells throughout the white matter. These cells were more ramified and abundant than cells positive for CD14 in white matter. Double staining for p24 and CD16 suggests that these cells were often infected with HIV-1. The prominent distribution of CD14+ cells in HIVE prompted our analysis of soluble CD14 levels in cerebrospinal fluid. Higher levels of soluble CD14 (sCD14) were observed in patients with moderate-to-severe HIV dementia, suggesting the utility of sCD14 as a surrogate marker. CD14+/CD16+ monocytes may play a role in other neurological disorders and sCD14 may be useful for evaluating these conditions.

  8. Accelerated mutation accumulation in asexual lineages of a freshwater snail.

    PubMed

    Neiman, Maurine; Hehman, Gery; Miller, Joseph T; Logsdon, John M; Taylor, Douglas R

    2010-04-01

    Sexual reproduction is both extremely costly and widespread relative to asexual reproduction, meaning that it must also confer profound advantages in order to persist. One theorized benefit of sex is that it facilitates the clearance of harmful mutations, which would accumulate more rapidly in the absence of recombination. The extent to which ineffective purifying selection and mutation accumulation are direct consequences of asexuality and whether the accelerated buildup of harmful mutations in asexuals can occur rapidly enough to maintain sex within natural populations, however, remain as open questions. We addressed key components of these questions by estimating the rate of mutation accumulation in the mitochondrial genomes of multiple sexual and asexual representatives of Potamopyrgus antipodarum, a New Zealand snail characterized by mixed sexual/asexual populations. We found that increased mutation accumulation is associated with asexuality and occurs rapidly enough to be detected in recently derived asexual lineages of P. antipodarum. Our results demonstrate that increased mutation accumulation in asexuals can differentially affect coexisting and ecologically similar sexual and asexual lineages. The accelerated rate of mutation accumulation observed in asexual P. antipodarum provides some of the most direct evidence to date for a link between asexuality and mutation accumulation and implies that mutational buildup could be rapid enough to contribute to the short-term evolutionary mechanisms that favor sexual reproduction.

  9. Adjustment for gas exchange threshold enhances precision of heart rate-derived VO2 estimates during heavy exercise.

    PubMed

    Pettitt, Robert W; Symons, J David; Taylor, Julie E; Eisenman, Patricia A; White, Andrea T

    2008-02-01

    Overestimates of oxygen uptake (VO2) are derived from the heart rate reserve-VO2 reserve (HRR-VO2R) model. We tested the hypothesis that adjusting for differences above and below gas exchange threshold (HRR-GET model) would tighten the precision of HR-derived VO2 estimates during heavy exercise. Seven men and 7 women of various VO2 max levels, on 2 separate days, cycled for 6 min at intensities equal to power at GET, 15% the difference between GET and VO2 max (15% above), and at 30% above GET. A second bout at 15% above GET (15% above (bout 2)) after 3 min of recovery was performed to assess estimates during interval training. Actual VO2 was compared with estimates derived from the HRR-VO2R and the HRR-GET. VO2 values were summed over the 6 min duration of data collection (6 min LO2) and compared with Bland-Altman plots. HRR-VO2R yielded 6 min LO2 (+/-2 SD) overestimates of 2.0 (+/-2.5), 1.9 (+/-2.7), and 1.3 (+/-3.3) for GET, 15% over, and 30% over, respectively, whereas corresponding 6 min LO2 difference values for the HRR-GET model were -0.42 (+/-1.6), -0.23 (+/-1.1), and -0.55 (+/-1.8), respectively. For 15% above (bout 2), the 6 min LO2 difference for HRR-VO2R was 1.8 (+/-2.9), whereas the difference for HRR-GET was 0.17 (+/-1.4). The 6 min LO2 values relative to the subjects' VO2 max did not vary (r=0.05 to 0.36); therefore, fitness level did not affect estimates. Sex did not affect accuracy of either estimate model (sex X estimate model interaction, p>0.95). We observed accurate estimates from the HRR-GET model during heavy exercise.

  10. Accumulation and conversion of sugars by developing wheat grains. VII. Effect of changes in sieve tube and endosperm cavity sap concentrations on the grain filling rate. [Triticum aestivum

    SciTech Connect

    Fisher, D.B.; Gifford, R.M.

    1987-06-01

    The extent to which wheat grain growth is dependent on transport pool solute concentration was investigated by the use of illumination and partial grain removal to vary solute concentrations in the sieve tube and endosperm cavity saps of the wheat ear (Triticum aestivum L.). Short-term grain growth rates were estimated indirectly from the product of phloem area, sieve tube sap concentration, and /sup 32/P translocation velocity. On a per grain basis, calculated rates of mass transport through the peduncle were fairly constant over a substantial range in other transport parameters (i.e. velocity, concentration, phloem area, and grain number). The rates were about 40% higher than expected; this probably reflects some unavoidable bias on faster-moving tracer in the velocity estimates. Sieve tube sap concentration increased in all experiments (by 20 to 64%), with a concomitant decline in velocity (to as low as 8% of the initial value). Endosperm cavity sucrose concentration also increased in all experiments, but cavity sap osmolality and total amino acid concentration remained nearly constant. No evidence was found for an increase in the rate of mass transport per grain through the peduncle in response to the treatments. This apparent unresponsiveness of grain growth rate to increased cavity sap sucrose concentration conflicts with earlier in vitro endosperm studies showing that sucrose uptake increased with increasing external sucrose concentration up to 150 to 200 millimolar.

  11. Gr-1intCD11b+ myeloid-derived suppressor cells accumulate in corneal allograft and improve corneal allograft survival.

    PubMed

    Choi, Wungrak; Ji, Yong Woo; Ham, Hwa-Yong; Yeo, Areum; Noh, Hyemi; Jin, Su-Eon; Song, Jong Suk; Kim, Hyeon Chang; Kim, Eung Kwon; Lee, Hyung Keun

    2016-12-01

    We identified the characteristics of myeloid-derived suppressor cells (MDSCs) and investigated their mechanism of induction and their functional role in allograft rejection using a murine corneal allograft model. In mice, MDSCs coexpress CD11b and myeloid differentiation antigen Gr-1. Gr-1(+)CD11b(+) cells infiltrated allografted corneas between 4 d and 4 wk after surgery; however, the frequencies of Gr-1(+)CD11b(+) cells were not different between accepted and rejected allografts or in peripheral blood or BM. Of interest, Gr-1(int)CD11b(+) cells, but not Gr-1(hi)CD11b(+) cells, infiltrated the accepted graft early after surgery and expressed high levels of immunosuppressive cytokines, including IL-10, TGF-β, and TNF-related apoptosis-inducing ligand. This population remained until 4 wk after surgery. In vitro, only high dose (>100 ng/ml) of IFN-γ plus GM-CSF could induce immunosuppressive cytokine expression in Gr-1(int)CD11b(+) cells. Furthermore, adoptive transfer of Gr-1(int)CD11b(+) cells reduced T cell infiltration, which improved graft survival. In conclusion, high-dose IFN-γ in allograft areas is essential for development of Gr-1(int)CD11b(+) MDSCs in corneal allografts, and subtle environmental changes in the early period of the allograft can result in a large difference in graft survival.

  12. Non-Saccharomyces and Saccharomyces strains co-fermentation increases acetaldehyde accumulation: effect on anthocyanin-derived pigments in Tannat red wines.

    PubMed

    Medina, Karina; Boido, Eduardo; Fariña, Laura; Dellacassa, Eduardo; Carrau, Francisco

    2016-07-01

    During fermentation, Saccharomyces cerevisiae releases into the medium secondary metabolic products, such as acetaldehyde, able to react with anthocyanins, producing more stable derived pigments. However, very limited reports are found about non-Saccharomyces effects on grape fermentation. In this study, six non-Saccharomyces yeast strains, belonging to the genera Metschnikowia and Hanseniaspora, were screened for their effect on red wine colour and wine-making capacity under pure culture conditions and mixed with Saccharomyces. An artificial red grape must was prepared, containing a phenolic extract of Tannat grapes that allows monitoring changes of key phenol parameters during fermentation, but without skin solids in the medium. When fermented in pure cultures, S. cerevisiae produced higher concentrations of acetaldehyde and vitisin B (acetaldehyde reaction-dependent) compared to M. pulcherrima M00/09G, Hanseniaspora guillermondii T06/09G, H. opuntiae T06/01G, H. vineae T02/05F and H. clermontiae (A10/82Fand C10/54F). However, co-fermentation of H. vineae and H. clermontiae with S. cerevisiae resulted in a significantly higher concentration of acetaldehyde compared with the pure S. cerevisiae control. HPLC-DAD-MS analysis confirmed an increased formation of vitisin B in co-fermentation treatments when compared to pure Saccharomyces fermentation, suggesting the key role of acetaldehyde. Copyright © 2016 John Wiley & Sons, Ltd.

  13. Derivation of Failure Rates and Probability of Failures for the International Space Station Probabilistic Risk Assessment Study

    NASA Technical Reports Server (NTRS)

    Vitali, Roberto; Lutomski, Michael G.

    2004-01-01

    National Aeronautics and Space Administration s (NASA) International Space Station (ISS) Program uses Probabilistic Risk Assessment (PRA) as part of its Continuous Risk Management Process. It is used as a decision and management support tool to not only quantify risk for specific conditions, but more importantly comparing different operational and management options to determine the lowest risk option and provide rationale for management decisions. This paper presents the derivation of the probability distributions used to quantify the failure rates and the probability of failures of the basic events employed in the PRA model of the ISS. The paper will show how a Bayesian approach was used with different sources of data including the actual ISS on orbit failures to enhance the confidence in results of the PRA. As time progresses and more meaningful data is gathered from on orbit failures, an increasingly accurate failure rate probability distribution for the basic events of the ISS PRA model can be obtained. The ISS PRA has been developed by mapping the ISS critical systems such as propulsion, thermal control, or power generation into event sequences diagrams and fault trees. The lowest level of indenture of the fault trees was the orbital replacement units (ORU). The ORU level was chosen consistently with the level of statistically meaningful data that could be obtained from the aerospace industry and from the experts in the field. For example, data was gathered for the solenoid valves present in the propulsion system of the ISS. However valves themselves are composed of parts and the individual failure of these parts was not accounted for in the PRA model. In other words the failure of a spring within a valve was considered a failure of the valve itself.

  14. Relationship between sedimentation rates and benthic impact on Maërl beds derived from fish farming in the Mediterranean.

    PubMed

    Sanz-Lázaro, Carlos; Belando, María Dolores; Marín-Guirao, Lázaro; Navarrete-Mier, Francisco; Marín, Arnaldo

    2011-02-01

    The aim of this work was to study the dispersion of particulate wastes derived from marine fish farming and correlate the data with the impact on the seabed. Carbon and nutrients were correlated with the physico-chemical parameters of the sediment and the benthic community structure. The sedimentation rates in the benthic system were 1.09, 0.09 and 0.13 g m⁻² day⁻¹ for particulate organic carbon (POC), particulate organic nitrogen (PON) and total phosphorus (TP), respectively. TP was a reliable parameter for establishing the spatial extent of the fish farm particulate wastes. Fish farming was seen to influence not only physico-chemical and biological parameters but also the functioning of the ecosystem from a trophic point of view, particularly affecting the grazers and the balance among the trophic groups. POC, PON and TP sedimentation dynamics reflected the physico-chemical status of the sediment along the distance gradient studied, while their impact on the benthic community extended further. Therefore, the level of fish farm impact on the benthic community might be underestimated if it is assessed by merely taking into account data obtained from waste dispersion rates. The benthic habitat beneath the fish farm, Maërl bed, was seen to be very sensitive to aquaculture impact compared with other unvegetated benthic habitats, with an estimated POC-carrying capacity to maintain current diversity of 0.087 g C m⁻² day⁻¹ (only 36% greater than the basal POC input). Environmental protection agencies should define different aquaculture waste load thresholds for different benthic communities affected by finfish farming, according to their particular degree of sensitivity, in order to maintain natural ecosystem functions.

  15. Accumulation of recombinant SARS-CoV spike protein in plant cytosol and chloroplasts indicate potential for development of plant-derived oral vaccines.

    PubMed

    Li, Hong-Ye; Ramalingam, Sathishkumar; Chye, Mee-Len

    2006-09-01

    Plants are promising candidates as bioreactors for the production of oral recombinant proteins in the biopharmaceutical industry. As an initial step toward provision of an oral vaccine against the severe acute respiratory syndrome coronavirus (SARS-CoV), we have expressed a partial spike (S) protein of SARS-CoV in the cytosol of nuclear-transformed plants and in the chloroplasts of plastid-transformed plants. In the construction of both nuclear and plastid transformation vectors, a 2-kilobase nucleotide sequence encoding amino acids 1-658 of the SARS-CoV spike protein (S1) was modified with nucleotide changes, but not amino acid changes, to optimize codon usage for expression in plants. To investigate the subcellular localization of S1 during transient expression in tobacco leaves, a translational fusion consisting of S1 and the green fluorescent protein (GFP) was generated. Following agroinfiltration of tobacco leaves, analysis by laser confocal scanning microscopy revealed that the S1:GFP fusion protein was localized to the cytosol. In stable transgenic tobacco plants and lettuce plants generated by Agrobacterium-mediated transformation, tobacco and lettuce leaves were observed to express the S1 at high levels from the Cauliflower Mosaic Virus 35S promoter with Northern blot analysis. When the S1 was expressed in transplastomic tobacco, S1 messenger RNA and its corresponding protein were detected on Northern and Western blot analyses, respectively. Our results demonstrate the feasibility of producing S1 in nuclear- and chloroplast-transformed plants, indicating its potential in subsequent development of a plant-derived and safe oral recombinant subunit vaccine against the SARS-CoV in edible plants.

  16. Nitrogen Limited Red and Green Leaf Lettuce Accumulate Flavonoid Glycosides, Caffeic Acid Derivatives, and Sucrose while Losing Chlorophylls, Β-Carotene and Xanthophylls.

    PubMed

    Becker, Christine; Urlić, Branimir; Jukić Špika, Maja; Kläring, Hans-Peter; Krumbein, Angelika; Baldermann, Susanne; Goreta Ban, Smiljana; Perica, Slavko; Schwarz, Dietmar

    2015-01-01

    Reduction of nitrogen application in crop production is desirable for ecological and health-related reasons. Interestingly, nitrogen deficiency can lead to enhanced concentrations of polyphenols in plants. The reason for this is still under discussion. The plants' response to low nitrogen concentration can interact with other factors, for example radiation intensity. We cultivated red and green leaf lettuce hydroponically in a Mediterranean greenhouse, supplying three different levels of nitrogen (12 mM, 3 mM, 0.75 mM), either in full or reduced (-50%) radiation intensity. In both red and green lettuce, we found clear effects of the nitrogen treatments on growth characteristics, phenolic and photosynthetic compounds, nitrogen, nitrate and carbon concentration of the plants. Interestingly, the concentrations of all main flavonoid glycosides, caffeic acid derivatives, and sucrose increased with decreasing nitrogen concentration, whereas those of chlorophylls, β-carotene, neoxanthin, lactucaxanthin, all trans- and cis-violaxanthin decreased. The constitutive concentrations of polyphenols were lower in the green cultivar, but their relative increase was more pronounced than in the red cultivar. The constitutive concentrations of chlorophylls, β-carotene, neoxanthin, all trans- and cis-violaxanthin were similar in red and green lettuce and with decreasing nitrogen concentration they declined to a similar extent in both cultivars. We only detected little influence of the radiation treatments, e.g. on anthocyanin concentration, and hardly any interaction between radiation and nitrogen concentration. Our results imply a greater physiological plasticity of green compared to the red lettuce regarding its phenolic compounds. They support the photoprotection theory regarding anthocyanins as well as the theory that the deamination activity of phenylalanine ammonia-lyase drives phenylpropanoid synthesis.

  17. Nitrogen Limited Red and Green Leaf Lettuce Accumulate Flavonoid Glycosides, Caffeic Acid Derivatives, and Sucrose while Losing Chlorophylls, Β-Carotene and Xanthophylls

    PubMed Central

    Becker, Christine; Urlić, Branimir; Jukić Špika, Maja; Kläring, Hans-Peter; Krumbein, Angelika; Baldermann, Susanne; Goreta Ban, Smiljana; Perica, Slavko; Schwarz, Dietmar

    2015-01-01

    Reduction of nitrogen application in crop production is desirable for ecological and health-related reasons. Interestingly, nitrogen deficiency can lead to enhanced concentrations of polyphenols in plants. The reason for this is still under discussion. The plants’ response to low nitrogen concentration can interact with other factors, for example radiation intensity. We cultivated red and green leaf lettuce hydroponically in a Mediterranean greenhouse, supplying three different levels of nitrogen (12 mM, 3 mM, 0.75 mM), either in full or reduced (-50%) radiation intensity. In both red and green lettuce, we found clear effects of the nitrogen treatments on growth characteristics, phenolic and photosynthetic compounds, nitrogen, nitrate and carbon concentration of the plants. Interestingly, the concentrations of all main flavonoid glycosides, caffeic acid derivatives, and sucrose increased with decreasing nitrogen concentration, whereas those of chlorophylls, β-carotene, neoxanthin, lactucaxanthin, all trans- and cis-violaxanthin decreased. The constitutive concentrations of polyphenols were lower in the green cultivar, but their relative increase was more pronounced than in the red cultivar. The constitutive concentrations of chlorophylls, β-carotene, neoxanthin, all trans- and cis-violaxanthin were similar in red and green lettuce and with decreasing nitrogen concentration they declined to a similar extent in both cultivars. We only detected little influence of the radiation treatments, e.g. on anthocyanin concentration, and hardly any interaction between radiation and nitrogen concentration. Our results imply a greater physiological plasticity of green compared to the red lettuce regarding its phenolic compounds. They support the photoprotection theory regarding anthocyanins as well as the theory that the deamination activity of phenylalanine ammonia-lyase drives phenylpropanoid synthesis. PMID:26569488

  18. Label-Free Kinetics: Exploiting Functional Hemi-Equilibrium to Derive Rate Constants for Muscarinic Receptor Antagonists.

    PubMed

    Riddy, Darren M; Valant, Celine; Rueda, Patricia; Charman, William N; Sexton, Patrick M; Summers, Roger J; Christopoulos, Arthur; Langmead, Christopher J

    2015-10-01

    Drug receptor kinetics is as a key component in drug discovery, development, and efficacy; however, determining kinetic parameters has historically required direct radiolabeling or competition with a labeled tracer. Here we present a simple approach to determining the kinetics of competitive antagonists of G protein-coupled receptors by exploiting the phenomenon of hemi-equilibrium, the state of partial re-equilibration of agonist, antagonist, and receptor in some functional assays. Using functional [Ca(2+)]i-flux and extracellular kinases 1 and 2 phosphorylation assays that have short incubation times and therefore are prone to hemi-equilibrium "behaviors," we investigated a wide range of structurally and physicochemically distinct muscarinic acetylcholine receptor antagonists. Using a combined operational and hemi-equilibrium model of antagonism to both simulate and analyze data, we derived estimates of association and dissociation rates for the test set of antagonists, identifying both rapidly dissociating (4-DAMP, himbacine) and slowly dissociating (tiotropium, glycopyrrolate) ligands. The results demonstrate the importance of assay incubation time and the degree of receptor reserve in applying the analytical model. There was an excellent correlation between estimates of antagonist pK(B), k(on), and k(off) from functional assays and those determined by competition kinetics using whole-cell [(3)H]N-methylscopolamine binding, validating this approach as a rapid and simple method to functionally profile receptor kinetics of competitive antagonists in the absence of a labeled tracer.

  19. C02(nu2)-0 Quenching Rate Coefficient Derived from Coincidental Fort Collins Lidar and SABER Measurements

    NASA Technical Reports Server (NTRS)

    Feofilov, A. G.; Kutepov, A. A.; She, C. Y.; Smith, A. K.; Pesnell, W. D.; Goldberg, R. A.

    2009-01-01

    Among the processes governing the energy balance in the mesosphere and lower thermosphere (MLT), the quenching of CO2(V2) vibrational levels in collisions with oxygen atoms plays an important role. However, neither the rate coefficient of this process (k(CO2O)) nor the atomic oxygen concentrations ([O]) in the MLT are well known. The discrepancy between k(CO2O) measured in the lab and retrieved from atmospheric measurements is of about factor of 2.5. At the same time, the discrepancy between [O] in the MLT measured by different instruments is of the same order of magnitude. In this work we used a synergy of a ground based lidar and satellite infrared radiometer to make a further step in understanding of the physics of the region. In this study we apply the night- and daytime temperatures between 80 and 110 km measured by the Colorado State University narrow-band sodium (Na) lidar located at Fort Collins, Colorado for retrieving the product of k(CO2-O) x [O] from the limb radiances in the 15 micron channel measured by the SABER/TIMED instrument for nearly simultaneous common volume measurements of both instruments within +/-1 degree in latitude, +/-2 degrees in longitude and +/-10 minutes in time. We derive k(CO2-O) and its possible variation range from the retrieved product by utilizing the [O] values measured by the SABER and other instruments.

  20. Ultraviolet to infrared emission of z > 1 galaxies: Can we derive reliable star formation rates and stellar masses?

    NASA Astrophysics Data System (ADS)

    Buat, V.; Heinis, S.; Boquien, M.; Burgarella, D.; Charmandaris, V.; Boissier, S.; Boselli, A.; Le Borgne, D.; Morrison, G.

    2014-01-01

    Aims: Our knowledge of the cosmic mass assembly relies on measurements of star formation rates (SFRs) and stellar masses (Mstar), of galaxies as a function of redshift. These parameters must be estimated in a consistent way with a good knowledge of systematics before studying their correlation and the variation of the specific SFR. Constraining these fundamental properties of galaxies across the Universe is of utmost importance if we want to understand galaxy formation and evolution. Methods: We seek to derive SFRs and stellar masses in distant galaxies and to quantify the main uncertainties affecting their measurement. We explore the impact of the assumptions made in their derivation with standard calibrations or through a fitting process, as well as the impact of the available data, focusing on the role of infrared emission originating from dust. Results: We build a sample of galaxies with z > 1, all observed from the ultraviolet to the infrared in their rest frame. The data are fitted with the code CIGALE, which is also used to build and analyse a catalogue of mock galaxies. Models with different star formation histories are introduced: an exponentially decreasing or increasing SFR and a more complex one coupling a decreasing SFR with a younger burst of constant star formation. We define different sets of data, with or without a good sampling of the ultraviolet range, near-infrared, and thermal infrared data. Variations of the metallicity are also investigated. The impact of these different cases on the determination of stellar mass and SFR are analysed. Conclusions: Exponentially decreasing models with a redshift formation of the stellar population zf ≃ 8 cannot fit the data correctly. All the other models fit the data correctly at the price of unrealistically young ages when the age of the single stellar population is taken to be a free parameter, especially for the exponentially decreasing models. The best fits are obtained with two stellar populations. As

  1. Combined Aircraft and Satellite-Derived Storm Electric Current and Lightning Rates Measurements and Implications for the Global Electric Circuit

    NASA Technical Reports Server (NTRS)

    Mach, Douglas M.; Blakeslee, Richard J.; Bateman, Monte G.

    2010-01-01

    Using rotating vane electric field mills and Gerdien capacitors, we measured the electric field profile and conductivity during 850 overflights of electrified shower clouds and thunderstorms spanning regions including the Southeastern United States, the Western Atlantic Ocean, the Gulf of Mexico, Central America and adjacent oceans, Central Brazil, and the South Pacific. The overflights include storms over land and ocean, with and without lightning, and with positive and negative fields above the storms. The measurements were made with the NASA ER-2 and the Altus-II high altitude aircrafts. Peak electric fields, with lightning transients removed, ranged from -1.0 kV/m to 16 kV/m, with a mean value of 0.9 kV/m. The median peak field was 0.29 kV/m. Integrating our electric field and conductivity data, we determined total conduction currents and flash rates for each overpass. With knowledge of the storm location (land or ocean) and type (with or without lightning), we determine the mean currents by location and type. The mean current for ocean storms with lightning is 1.6 A while the mean current for land storms with lightning is 1.0 A. The mean current for oceanic storms without lightning (i.e., electrified shower clouds) is 0.39 A and the mean current for land storms without lightning is 0.13 A. Thus, on average, land storms with or without lightning have about half the mean current as their corresponding oceanic storm counterparts. Over three-quarters (78%) of the land storms had detectable lightning, while less than half (43%) of the oceanic storms had lightning. We did not find any significant regional or latitudinal based patterns in our total conduction currents. By combining the aircraft derived storm currents and flash rates with diurnal lightning statistics derived from the Lightning Imaging Sensor (LIS) and Optical Transient Detector (OTD) low Earth orbiting satellites, we reproduce the diurnal variation in the global electric circuit (i.e., the Carnegie

  2. A first attempt to derive soil erosion rates from 137Cs airborne gamma measurements in two Alpine valleys

    NASA Astrophysics Data System (ADS)

    Arata, Laura; Meusburger, Katrin; Bucher, Benno; Mabit, Lionel; Alewell, Christine

    2016-04-01

    The application of fallout radionuclides (FRNs) as soil tracers is currently one of the most promising and effective approach for evaluating soil erosion magnitudes in mountainous grasslands. Conventional assessment or measurement methods are laborious and constrained by the topographic and climatic conditions of the Alps. The 137Cs (half-life = 30.2 years) is the most frequently used FRN to study soil redistribution. However the application of 137Cs in alpine grasslands is compromised by the high heterogeneity of the fallout due to the origin of 137Cs fallout in the Alps, which is linked to single rain events occurring just after the Chernobyl accident when most of the Alpine soils were still covered by snow. The aim of this study was to improve our understanding of the 137Cs distribution in two study areas in the Central Swiss Alps: the Ursern valley (Canton Uri), and the Piora valley (Canton Ticino). In June 2015, a helicopter equipped with a NaI gamma detector flew over the two study sites and screened the 137Cs activity of the top soil. The use of airborne gamma measurements is particularly efficient in case of higher 137Cs concentration in the soil. Due to their high altitude and high precipitation rates, the Swiss Alps are expected to be more contaminated by 137Cs fallout than other parts of Switzerland. The airborne gamma measurements have been related to several key parameters which characterize the areas, such as soil properties, slopes, expositions and land uses. The ground truthing of the airborne measurements (i.e. the 137Cs laboratory measurements of the soil samples collected at the same points) returned a good fit. The obtained results offer an overview of the 137Cs concentration in the study areas, which allowed us to identify suitable reference sites, and to analyse the relationship between the 137Cs distribution and the above cited parameters. The authors also derived a preliminary qualitative and a quantitative assessment of soil redistribution

  3. Controlling the degradation rate of AZ91 magnesium alloy via sol-gel derived nanostructured hydroxyapatite coating.

    PubMed

    Rojaee, Ramin; Fathi, Mohammadhossein; Raeissi, Keyvan

    2013-10-01

    Magnesium (Mg) alloys have been introduced as new generation of biodegradable orthopedic materials in recent years since it has been proved that Mg is one of the main minerals required for osseous tissue revival. The main goal of the present study was to establish a desired harmony between the necessities of orthopedic patient body to Mg(2+) ions and degradation rate of the Mg based implants as a new class of biodegradable/bioresorbable materials. This prospect was followed by providing a sol-gel derived nanostructured hydroxyapatite (n-HAp) coating on AZ91 alloy using dip coating technique. Phase structural analysis, morphology study, microstructure characterization, and functional group identification were performed using X-ray diffraction (XRD), Fourier transform infrared (FTIR), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) techniques. The prepared samples were immersed in simulated body fluid in order to study the formation of apatite-like precipitations, barricade properties of the n-HAp coating, and to estimate the dosage of released Mg(2+) ions within a specified and limited time of implantation. Electrochemical polarization tests were carried out to evaluate and compare the corrosion behavior of the n-HAp coated and uncoated samples. The changes of the in vitro pH values were also evaluated. Results posed the noticeable capability of n-HAp coating on stabilizing alkalization behavior and improving the corrosion resistance of AZ91 alloy. It was concluded that n-HAp coated AZ91 alloy could be a good candidate as a type of biodegradable implant material for biomedical applications.

  4. A review of methods for the signal quality assessment to improve reliability of heart rate and blood pressures derived parameters.

    PubMed

    Gambarotta, Nicolò; Aletti, Federico; Baselli, Giuseppe; Ferrario, Manuela

    2016-07-01

    The assessment of signal quality has been a research topic since the late 1970s, as it is mainly related to the problem of false alarms in bedside monitors in the intensive care unit (ICU), the incidence of which can be as high as 90 %, leading to alarm fatigue and a drop in the overall level of nurses and clinicians attention. The development of efficient algorithms for the quality control of long diagnostic electrocardiographic (ECG) recordings, both single- and multi-lead, and of the arterial blood pressure (ABP) signal is therefore essential for the enhancement of care quality. The ECG signal is often corrupted by noise, which can be within the frequency band of interest and can manifest similar morphologies as the ECG itself. Similarly to ECG, also the ABP signal is often corrupted by non-Gaussian, nonlinear and non-stationary noise and artifacts, especially in ICU recordings. Moreover, the reliability of several important parameters derived from ABP such as systolic blood pressure or pulse pressure is strongly affected by the quality of the ABP waveform. In this work, several up-to-date algorithms for the quality scoring of a single- or multi-lead ECG recording, based on time-domain approaches, frequency-domain approaches or a combination of the two will be reviewed, as well as methods for the quality assessment of ABP. Additionally, algorithms exploiting the relationship between ECG and pulsatile signals, such as ABP and photoplethysmographic recordings, for the reduction in the false alarm rate will be presented. Finally, some considerations will be drawn taking into account the large heterogeneity of clinical settings, applications and goals that the reviewed algorithms have to deal with.

  5. Simulations of Methane Hydrate Phenomena Over Geologic Timescales. Part I: Effect of Sediment Compaction Rates on Methans Hydrate and Free Gas Accumulation

    SciTech Connect

    Gering, Kevin Leslie

    2003-01-01

    The focus of this work is a model that describes migration and biogenic formation of methane under conditions representative of dynamic marine basins, and the conversion of soluble methane into either solid hydrate or exsolved gas. Incorporated into the overall model are an accurate phase equilibria model for seawater-methane, a methane source term based on biogenesis data, and a sediment compaction model based on porosity as a function of position, time, and the local volume fractions of hydrate solids and free gas. Simulations have shown that under some compaction scenarios, liquid overpressures reach the lithostatic limit due to permeability constraints, which can diminish the advective transfer of soluble methane within the porous sediment. As such, the formation of methane hydrate can be somewhat of a self-moderating process. The occurrence and magnitude of hydrate formation is directly tied to fundamental parameters such as the compaction/sedimentation rates, liquid advection rates, seafloor depth, geothermal gradient, etc. Results are shown for simulations covering 20 million years, wherein growth profiles for methane hydrate and free gas (neither exceeding 10 vol% at any location) are tracked within a vertical sediment column spanning over 3000 m. A case study is also presented for the Blake Ridge region (Ocean Drilling Program Leg 164, Sites 994, 995, and 997) based on simulations covering 6 Ma, wherein it is concluded that methane migration from compaction-driven advection may account for 15-30% of the total hydrate mass present in this region.

  6. Changes in growth rate and macroelement and trace element accumulation in Hydrocharis morsus-ranae L. during the growing season in relation to environmental contamination.

    PubMed

    Polechońska, Ludmiła; Samecka-Cymerman, Aleksandra; Dambiec, Małgorzata

    2017-02-01

    The temporal variations in plant chemistry connected with its life cycle may affect the cycling of elements in an ecosystem as well as determine the usefulness of the species in phytoremediation and bioindication. In this context, there is a gap in knowledge on the role of floating plants for elements cycling in aquatic reservoirs. The aim of the study was to determine if there are variations in Hydrocharis morsus-ranae (European frog-bit) bioaccumulation capacity and the growth rate of its population during the growing season and to test the impact of environmental pollution on these features. The content of macroelements (Ca, K, Mg, N, Na, P, S) and trace metals (Cd, Co, Cu, Cr, Hg, Fe, Mn, Ni, Pb, Zn) was determined in H. morsus-ranae collected monthly from June to October from habitats differing in environmental contamination. The results showed that the highest content of most trace metals (Co, Cr, Cu, Hg, Mn, Ni, Zn) and some nutrients (N, P) in plants as well as the greatest bioaccumulation efficiency occurred simultaneously in the beginning of the growing season. In the following months, a dilution effect (manifested by a decrease in content) related to the rapid growth was observed. Co, Mn, and Ni content in plant tissues reflected the level of environmental contamination throughout the growing season which makes H. morsus-ranae a potential biomonitor of pollution for these metals. Considering the great bioaccumulation ability, high sensitivity to contamination, and low biomass of European frog-bit in polluted systems, further investigation is required to assess the real phytoremediation capability of the species.

  7. Effect of GABA derivatives on the rate of thrombus formation, platelet aggregation, and plasma coagulation capacity in rats with experimental gestosis.

    PubMed

    Tyurenkov, I N; Perfilova, V N; Karamysheva, V I; Reznikova, L B; Mokrousov, I S; Mikhailova, L I; Berestovitskaya, V M; Vasil'eva, O S

    2014-12-01

    Experimental gestosis induced by replacement of drinking water with 1.8% NaCl promoted hypercoagulation, increased the rate and degree of platelet aggregation, and reduced clotting time in pregnant females. GABA derivatives, compounds RGPU-151, RGPU-152, and phenibut normalized parameters of hemostasis and platelet aggregation and the rate of thrombus formation in the animals. The efficiency of the test substances did not significantly differ from that of the reference drug sulodexide.

  8. Validation of heart rate derived from a physiological status monitor-embedded compression shirt against criterion ECG.

    PubMed

    Dolezal, B A; Boland, D M; Carney, J; Abrazado, M; Smith, D L; Cooper, C B

    2014-01-01

    Firefighters are subject to extreme environments and high physical demands when performing duty-related tasks. Recently, physiological status monitors (PSM) have been embedded into a compression shirt to enable firefighters to measure, visualize, log, and transmit vital metrics such as heart rate (HR) to aid in cardiovascular risk identification and mitigation, thereby attempting to improve the health, fitness, and safety of this population. The purpose of this study was to validate HR recorded by the PSM-embedded compression shirt against a criterion standard laboratory ECG-derived HR when worn concurrently with structural firefighting personal protective equipment (PPE) during four simulated firefighting activities. Ten healthy, college-age men (mean ± SD: age: 21 ± 1 yr; body mass: 91 ± 10 kg; body mass index: 26.9 ± 3.1 kg/m(2)) completed four tasks that are routinely performed during firefighting operations: outdoor fast-paced walking (FW), treadmill walking (TW), searching/crawling (SC), and ascending/descending stairs (AD). They wore the PSM-embedded compression shirt under structural firefighting PPE. HR was recorded concurrently by the PSM-embedded compression shirt and a portable metabolic measurement system accompanied with a standard 12-lead electrocardiograph that was used to provide criterion measures of HR. For all four tasks combined there was very high correlation of PSM and ECG HR (r > 0.99; SEE 0.84 /min) with a mean difference (bias) of -0.02 /min and limits of agreement of -0.07 to 0.02 /min. For individual tasks, the correlations were also high (r-values = 0.99; SEE 0.81-0.89). The mean bias (limits of agreement) was: FW 0.03 (-0.09 to 0.14); TW 0.04 (-0.05 to 0.12); SC -0.01 (-0.12 to 0.10); AD -0.13 (-0.21 to -0.04) /min. These findings demonstrate that the PSM-embedded compression shirt provides a valid measure of HR during simulated firefighting activities when compared with a standard 12-lead ECG.

  9. Mass accumulation rate of detrital materials in Lake Suigetsu as a potential proxy for heavy precipitation: a comparison of the observational precipitation and sedimentary record

    NASA Astrophysics Data System (ADS)

    Suzuki, Yoshiaki; Tada, Ryuji; Yamada, Kazuyoshi; Irino, Tomohisa; Nagashima, Kana; Nakagawa, Takeshi; Omori, Takayuki

    2016-02-01

    In the densely populated region of East Asia, it is important to know the mechanism, scale, and frequency of heavy precipitation brought about during the monsoons and typhoons. However, observational data, which cover only several decades, are insufficient to examine the long-term trend of extreme precipitation and its background mechanism. In humid areas, the transport flux of a suspended detrital material through a river system is known to have an empirical power relationship with precipitation. Thus, the sedimentation flux of a fine detrital material could potentially be used as a proxy for reconstructing past heavy precipitation events. To test the idea that the sedimentation flux of detrital materials records past heavy precipitation events (e.g., typhoons), we focused on the detrital flux estimated from the annually laminated sediment of Lake Suigetsu, central Japan, which is capable of accurately correlating the age of detrital flux with the precipitation record. We first established a precise age model (error within ±1 year in average) beginning in 1920 A.D. on the basis of varve counting fine-tuned by correlation between event layers with historical floods. The flux of the detrital material (g/cm2/year) was estimated on the basis of Al2O3 content (wt%), dry bulk density (g/cm3), and sedimentation rate (cm/year) calculated from the age model. The detrital flux of background sedimentation showed a weak positive correlation with annual and monthly (June and September) precipitation excluding heavy precipitation that exceeded 100 mm/day. Furthermore, the thickness of instantaneous event layers, which corresponds to several maxima of detrital flux and is correlated with floods that occurred mainly during typhoons, showed a positive relationship with the total amount of precipitation that caused a flood event. This result suggests that the detrital flux maxima (deposition of event layers) record past extreme precipitation events that were likely associated with

  10. 34 CFR 222.69 - What tax rates may the Secretary use if substantial local revenues are derived from local tax...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... local revenues are derived from local tax sources other than real property taxes? 222.69 Section 222.69... Educational Agencies Under Section 8003(f) of the Act § 222.69 What tax rates may the Secretary use if... making a reasonable tax effort under § 222.66. (b) If, based upon the request of an SEA, the...

  11. Strain accumulation in quasicrystalline solids

    NASA Technical Reports Server (NTRS)

    Nori, Franco; Ronchetti, Marco; Elser, Veit

    1988-01-01

    The relaxation of two-dimensional quasicrystalline elastic networks when their constituent bonds are perturbed homogeneously is studied. Whereas ideal, quasi-periodic networks are stable against such perturbations, significant accumulations of strain in a class of disordered networks generated by a growth process are found. The grown networks are characterized by root mean square phason fluctuations which grow linearly with system size. The strain accumulation observed in these networks also grows linearly with system size. Finally, dependence of strain accumulation on cooling rate is found.

  12. An accurate derivation of the air dose-rate and the deposition concentration distribution by aerial monitoring in a low level contaminated area

    NASA Astrophysics Data System (ADS)

    Nishizawa, Yukiyasu; Sugita, Takeshi; Sanada, Yukihisa; Torii, Tatsuo

    2015-04-01

    Since 2011, MEXT (Ministry of Education, Culture, Sports, Science and Technology, Japan) have been conducting aerial monitoring to investigate the distribution of radioactive cesium dispersed into the atmosphere after the accident at the Fukushima Dai-ichi Nuclear Power Plant (FDNPP), Tokyo Electric Power Company. Distribution maps of the air dose-rate at 1 m above the ground and the radioactive cesium deposition concentration on the ground are prepared using spectrum obtained by aerial monitoring. The radioactive cesium deposition is derived from its dose rate, which is calculated by excluding the dose rate of the background radiation due to natural radionuclides from the air dose-rate at 1 m above the ground. The first step of the current method of calculating the dose rate due to natural radionuclides is calculate the ratio of the total count rate of areas where no radioactive cesium is detected and the count rate of regions with energy levels of 1,400 keV or higher (BG-Index). Next, calculate the air dose rate of radioactive cesium by multiplying the BG-Index and the integrated count rate of 1,400 keV or higher for the area where the radioactive cesium is distributed. In high dose-rate areas, however, the count rate of the 1,365-keV peak of Cs-134, though small, is included in the integrated count rate of 1,400 keV or higher, which could cause an overestimation of the air dose rate of natural radionuclides. We developed a method for accurately evaluating the distribution maps of natural air dose-rate by excluding the effect of radioactive cesium, even in contaminated areas, and obtained the accurate air dose-rate map attributed the radioactive cesium deposition on the ground. Furthermore, the natural dose-rate distribution throughout Japan has been obtained by this method.

  13. Greenland annual accumulation along the EGIG line, 1959-2004, from ASIRAS airborne radar and neutron-probe density measurements

    NASA Astrophysics Data System (ADS)

    Overly, Thomas B.; Hawley, Robert L.; Helm, Veit; Morris, Elizabeth M.; Chaudhary, Rohan N.

    2016-08-01

    We report annual snow accumulation rates from 1959 to 2004 along a 250 km segment of the Expéditions Glaciologiques Internationales au Groenland (EGIG) line across central Greenland using Airborne SAR/Interferometric Radar Altimeter System (ASIRAS) radar layers and high resolution neutron-probe (NP) density profiles. ASIRAS-NP-derived accumulation rates are not statistically different (95 % confidence interval) from in situ EGIG accumulation measurements from 1985 to 2004. ASIRAS-NP-derived accumulation increases by 20 % below 3000 m elevation, and increases by 13 % above 3000 m elevation for the period 1995 to 2004 compared to 1985 to 1994. Three Regional Climate Models (PolarMM5, RACMO2.3, MAR) underestimate snow accumulation below 3000 m by 16-20 % compared to ASIRAS-NP from 1985 to 2004. We test radar-derived accumulation rates sensitivity to density using modeled density profiles in place of NP densities. ASIRAS radar layers combined with Herron and Langway (1980) model density profiles (ASIRAS-HL) produce accumulation rates within 3.5 % of ASIRAS-NP estimates in the dry snow region. We suggest using Herron and Langway (1980) density profiles to calibrate radar layers detected in dry snow regions of ice sheets lacking detailed in situ density measurements, such as those observed by the Operation IceBridge campaign.

  14. 43 CFR 3211.19 - What is the royalty rate on byproducts derived from geothermal resources produced from or...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... derived from geothermal resources produced from or attributable to my lease? 3211.19 Section 3211.19..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE LEASING Filing and Processing Fees... geothermal resources produced from or attributable to my lease? (a) For leases issued on or after August...

  15. 43 CFR 3211.19 - What is the royalty rate on byproducts derived from geothermal resources produced from or...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... derived from geothermal resources produced from or attributable to my lease? 3211.19 Section 3211.19..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE LEASING Filing and Processing Fees... geothermal resources produced from or attributable to my lease? (a) For leases issued on or after August...

  16. 43 CFR 3211.19 - What is the royalty rate on byproducts derived from geothermal resources produced from or...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... derived from geothermal resources produced from or attributable to my lease? 3211.19 Section 3211.19..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE LEASING Filing and Processing Fees... geothermal resources produced from or attributable to my lease? (a) For leases issued on or after August...

  17. 43 CFR 3211.19 - What is the royalty rate on byproducts derived from geothermal resources produced from or...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... derived from geothermal resources produced from or attributable to my lease? 3211.19 Section 3211.19..., DEPARTMENT OF THE INTERIOR MINERALS MANAGEMENT (3000) GEOTHERMAL RESOURCE LEASING Filing and Processing Fees... geothermal resources produced from or attributable to my lease? (a) For leases issued on or after August...

  18. Modelling Deposition and Erosion rates with RadioNuclides (MODERN) - Part 1: A new conversion model to derive soil redistribution rates from inventories of fallout radionuclides.

    PubMed

    Arata, Laura; Meusburger, Katrin; Frenkel, Elena; A'Campo-Neuen, Annette; Iurian, Andra-Rada; Ketterer, Michael E; Mabit, Lionel; Alewell, Christine

    2016-10-01

    The measurement of fallout radionuclides (FRN) has become one of the most commonly used tools to quantify sediment erosion or depositional processes. The conversion of FRN inventories into soil erosion and deposition rates is done with a variety of models, which suitability is dependent on the selected FRN, soil cultivation (ploughed or unploughed) and movement (erosion or deposition). The authors propose a new conversion model, which can be easily and comprehensively used for different FRN, land uses and soil redistribution processes. The new model MODERN (Modelling Deposition and Erosion rates with RadioNuclides) considers the precise depth distribution of any FRN at the reference site, and allows adapting it for any specific site conditions. MODERN adaptability and performance in converting different FRN inventories is discussed for a theoretical case as well as for two already published case studies i.e. a (137)Cs study in an alpine and unploughed area in the Aosta valley (Italy) and a (210)Pbex study on a ploughed area located in the Transylvanian Plain (Romania). The tests highlight a highly significant correspondence (i.e. correlation factor of 0.91) between the results of MODERN and the published results of other models currently used by the FRN scientific community (i.e. the Profile Distribution Model and the Mass Balance Model). The development and the cost free accessibility of MODERN (see modern.umweltgeo.unibas.ch) will ensure the promotion of wider application of FRNs for tracing soil erosion and sedimentation.

  19. Plant, cell, and molecular mechanisms of abscisic-acid regulation of stomatal apertures. A new mechanism for the regulation of stomatal-aperture size in intact leaves: Accumulation of mesophyll-derived sucrose in the guard-cell wall of Vicia faba L.

    SciTech Connect

    Lu, P.; Outlaw, W.H. Jr.; Smith, B.G.; Freed, G.A.

    1996-12-31

    At various times after pulse labeling Vicia faba L. leaflets with {sup 14}CO{sub 2}, whole-leaf pieces and rinsed epidermal peels were harvested and subsequently processed for histochemical analysis. Cells dissected from whole leaf retained apoplastic contents whereas those from rinsed peels contained only cytoplastic contents. Sucrose specific radioactivity peaked in palisade cells, 111 GBq{center_dot}mol{sup {minus}1}, at 20 min. In contrast, the {sup 14}C content and sucrose specific radioactivity were very low in guard cells for 20 min, implying little CO{sub 2} incorporation; both then peaked at 40 min. The guard-cell apoplast had a high maximum sucrose specific radioactivity and a high sucrose influx rate. These and other comparisons implied the presence of (a) multiple sucrose pools in mesophyll cells, (b) a localized mesophyll-apoplast region that exchanges with phloem and stomata, and (c) mesophyll-derived sucrose in guard-cell walls sufficient to diminish stomatal opening by {approximately} 4 {micro}m. Factors expected to enhance sucrose accumulation in guard-cell walls are (a) high transpiration rate, which closes stomata, and (b) high apoplastic sucrose concentration, which is elevated when mesophyll-sucrose efflux exceeds translocation. Therefore, multiple physiological factors are integrated in the attenuation of stomatal-aperture size by this previously unrecognized mechanism.

  20. Deleterious mutation accumulation in asexual Timema stick insects.

    PubMed

    Henry, Lee; Schwander, Tanja; Crespi, Bernard J

    2012-01-01

    Sexual reproduction is extremely widespread in spite of its presumed costs relative to asexual reproduction, indicating that it must provide significant advantages. One postulated benefit of sex and recombination is that they facilitate the purging of mildly deleterious mutations, which would accumulate in asexual lineages and contribute to their short evolutionary life span. To test this prediction, we estimated the accumulation rate of coding (nonsynonymous) mutations, which are expected to be deleterious, in parts of one mitochondrial (COI) and two nuclear (Actin and Hsp70) genes in six independently derived asexual lineages and related sexual species of Timema stick insects. We found signatures of increased coding mutation accumulation in all six asexual Timema and for each of the three analyzed genes, with 3.6- to 13.4-fold higher rates in the asexuals as compared with the sexuals. In addition, because coding mutations in the asexuals often resulted in considerable hydrophobicity changes at the concerned amino acid positions, coding mutations in the asexuals are likely associated with more strongly deleterious effects than in the sexuals. Our results demonstrate that deleterious mutation accumulation can differentially affect sexual and asexual lineages and support the idea that deleterious mutation accumulation plays an important role in limiting the long-term persistence of all-female lineages.

  1. Cattle stocking rates estimated in temperate intensive grasslands with a spring growth model derived from MODIS NDVI time-series

    NASA Astrophysics Data System (ADS)

    Green, Stuart; Cawkwell, Fiona; Dwyer, Edward

    2016-10-01

    There is an identified need for high resolution animal stocking rate data in temperate grassland systems. Here is presented a 250 m scale characterization of early spring vegetation growth (DOY 32-DOY 120) from 2003 to 2012 based on MODIS NDVI products for this period for Ireland. The average rate of grass growth is determined locally as a simple linear model for each pixel, using only the highest quality data for the period. These decadal spring growth model coefficients, start of season cover and growth rate, are regressed against log of stocking rate (r2 = 0.75). This model stocking rate is used to map grassland use intensity in Ireland, which, when tested against an independent set of stocking rate data, is shown to be successful with an RMSE error of 0.13 for a range of stocking densities from 0.1 to 3.0 LSU/Ha. This model provides the first validated high resolution approach to mapping stocking rates in intensively managed European grassland systems.

  2. Selenium accumulation by plants

    PubMed Central

    White, Philip J.

    2016-01-01

    Background Selenium (Se) is an essential mineral element for animals and humans, which they acquire largely from plants. The Se concentration in edible plants is determined by the Se phytoavailability in soils. Selenium is not an essential element for plants, but excessive Se can be toxic. Thus, soil Se phytoavailability determines the ecology of plants. Most plants cannot grow on seleniferous soils. Most plants that grow on seleniferous soils accumulate <100 mg Se kg–1 dry matter and cannot tolerate greater tissue Se concentrations. However, some plant species have evolved tolerance to Se, and commonly accumulate tissue Se concentrations >100 mg Se kg–1 dry matter. These plants are considered to be Se accumulators. Some species can even accumulate Se concentrations of 1000–15 000 mg Se kg–1 dry matter and are called Se hyperaccumulators. Scope This article provides an overview of Se uptake, translocation and metabolism in plants and highlights the possible genetic basis of differences in these between and within plant species. The review focuses initially on adaptations allowing plants to tolerate large Se concentrations in their tissues and the evolutionary origin of species that hyperaccumulate Se. It then describes the variation in tissue Se concentrations between and within angiosperm species and identifies genes encoding enzymes limiting the rates of incorporation of Se into organic compounds and chromosomal loci that might enable the development of crops with greater Se concentrations in their edible portions. Finally, it discusses transgenic approaches enabling plants to tolerate greater Se concentrations in the rhizosphere and in their tissues. Conclusions The trait of Se hyperaccumulation has evolved several times in separate angiosperm clades. The ability to tolerate large tissue Se concentrations is primarily related to the ability to divert Se away from the accumulation of selenocysteine and selenomethionine, which might be incorporated

  3. Sexual selection and maintenance of sex: evidence from comparisons of rates of genomic accumulation of mutations and divergence of sex-related genes in sexual and hermaphroditic species of Caenorhabditis.

    PubMed

    Artieri, Carlo G; Haerty, Wilfried; Gupta, Bhagwati P; Singh, Rama S

    2008-05-01

    Several hypotheses have been proposed to explain the persistence of dioecy despite the reproductive advantages conferred to hermaphrodites, including greater efficiency at purging deleterious mutations in the former. Dioecy can benefit from both mutation purging and accelerated evolution by bringing together beneficial mutations in the same individual via recombination and shuffling of genotypes. In addition, mathematical treatment has shown that sexual selection is also capable of mitigating the cost of maintaining separate sexes by increasing the overall fitness of sexual populations, and genomic comparisons have shown that sexual selection can lead to accelerated evolution. Here, we examine the advantages of dioecy versus hermaphroditism by comparing the rate of evolution in sex-related genes and the rate of accumulation of deleterious mutations using a large number of orthologs (11,493) in the dioecious Caenorhabditis remanei and the hermaphroditic Caenorhabditis briggsae. We have used this data set to estimate the deleterious mutation rate per generation, U, in both species and find that although it is significantly higher in hermaphrodites, both species are at least 2 orders of magnitude lower than the value required to explain the persistence of sex by efficiency at purging deleterious mutations alone. We also find that genes expressed in sperm are evolving rapidly in both species; however, they show a greater increase in their rate of evolution relative to genes expressed in other tissues in C. remanei, suggesting stronger sexual selection pressure acting on these genes in dioecious species. Interestingly, the persistence of a signal of rapid evolution of sperm genes in C. briggsae suggests a recent evolutionary origin of hermaphrodism in this lineage. Our results provide empirical evidence of increased sexual selection pressure in dioecious animals, supporting the possibility that sexual selection may play an important role in the maintenance of sexual

  4. Analytic expressions for perturbations and partial derivatives of range and range rate of a spacecraft with respect to the coefficient of the second harmonic

    NASA Technical Reports Server (NTRS)

    Georgevic, R. M.

    1973-01-01

    Closed-form analytic expressions for the time variations of instantaneous orbital parameters and of the topocentric range and range rate of a spacecraft moving in the gravitational field of an oblate large body are derived using a first-order variation of parameters technique. In addition, the closed-form analytic expressions for the partial derivatives of the topocentric range and range rate are obtained, with respect to the coefficient of the second harmonic of the potential of the central body (J sub 2). The results are applied to the motion of a point-mass spacecraft moving in the orbit around the equatorially elliptic, oblate sun, with J sub 2 approximately equal to .000027.

  5. Spatial variability of 10Be-derived erosion rates across the southern Peninsular Indian escarpment: A key to landscape evolution across passive margins

    NASA Astrophysics Data System (ADS)

    Mandal, Sanjay Kumar; Lupker, Maarten; Burg, Jean-Pierre; Valla, Pierre G.; Haghipour, Negar; Christl, Marcus

    2015-09-01

    The persistence of significant topography in ancient, tectonically inactive orogenic belts remains one of the outstanding questions in geomorphology. In southern Peninsular India, the impressive topographic relief of the Western Ghat Mountains in tectonic quiescence since at least ca. 65 Ma has raised important questions concerning the long-term mechanism of topographic evolution. Quantifying the distribution of erosion in space and time is critical to understanding landscape evolution. Although the long-term erosion rates are reasonably well known, the short-term erosion rates and the relative importance of factors controlling erosion in southern Peninsular India are less well constrained. We present a new suite of catchment-averaged and local erosion rates using in situ produced 10Be concentrations in river sediments and exposed bedrock samples in southern Peninsular India. Catchment-averaged erosion rates vary from 9.6 ± 0.8 mMa-1 in the highlands to 114.3 ± 13.8 mMa-1 on the escarpment side. Bedrock erosion rates range from 2.4 ± 0.2 mMa-1 in the ridge-top to 143.4 ± 25.4 mMa-1 in active channel beds of the highlands. Catchment-averaged erosion rates derived from the across-escarpment, westward-draining catchments are significantly higher than those derived from the eastward-draining, over highland catchments. The difference indicates that long-term down-wearing of the highland proceeds at lower rates than in the escarpment zones. Catchment-averaged erosion rates are moderately correlated with mean hillslope angles and local relief whereas they are strongly correlated with catchment-averaged channel steepness index. This suggests that topographic steepness is the major control on the spatial variability of erosion while strong rainfall gradient is of minor importance in this area. 10Be-derived average erosion rates in highlands are consistent with previous long-term erosion rate estimated from thermochronometry. These results collectively point to large

  6. Effect of application rate of commercial lignite-derived amendments on early-stage growth of Medicago sativa and soil health, in acidic soil conditions

    NASA Astrophysics Data System (ADS)

    Patti, Antonio; Little, Karen; Rose, Michael; Jackson, Roy; Cavagnaro, Tim

    2013-04-01

    Commercially available lignite-derived amendments, sold mainly as humate preparations, have been promoted as plant growth stimulants leading to higher crop yields. These products are also claimed to improve soil properties such as pH. This study investigated the effect of application rate of three lignite-derived amendments on the early-stage growth of a pasture legume, lucerne (Medicago sativa), and soil health in a soil type common to south eastern Australia, in a glasshouse setting. An organic-mineral humate product and 'run of mine' lignite coal did not improve shoot or root growth despite application at a range of rates at, and in excess of, the manufacturers recommendation. Application of soluble K-humate product at 20 kg/ha (9.5 kg/ha C equivalent) produced an observable positive effect in shoot growth. At this application rate, a significant delay in the appearance of chlorotic symptoms was observed along with an increase in soil pH concurrent with decreased availability of soil Mn and Al. Higher root dry weight was associated with lower microbial biomass carbon which may indicate an effect on allocation of resources between the microbial community and the plant. An assessment of the effectiveness of lignite-derived amendments on plant growth, as well as their potential to improve the health of an acidic soil will assist farmers in making decisions regarding the use of these products.

  7. Response times from ensembles of accumulators

    PubMed Central

    Zandbelt, Bram; Purcell, Braden A.; Palmeri, Thomas J.; Logan, Gordon D.; Schall, Jeffrey D.

    2014-01-01

    Decision-making is explained by psychologists through stochastic accumulator models and by neurophysiologists through the activity of neurons believed to instantiate these models. We investigated an overlooked scaling problem: How does a response time (RT) that can be explained by a single model accumulator arise from numerous, redundant accumulator neurons, each of which individually appears to explain the variability of RT? We explored this scaling problem by developing a unique ensemble model of RT, called e pluribus unum, which embodies the well-known dictum “out of many, one.” We used the e pluribus unum model to analyze the RTs produced by ensembles of redundant, idiosyncratic stochastic accumulators under various termination mechanisms and accumulation rate correlations in computer simulations of ensembles of varying size. We found that predicted RT distributions are largely invariant to ensemble size if the accumulators share at least modestly correlated accumulation rates and RT is not governed by the most extreme accumulators. Under these regimes the termination times of individual accumulators was predictive of ensemble RT. We also found that the threshold measured on individual accumulators, corresponding to the firing rate of neurons measured at RT, can be invariant with RT but is equivalent to the specified model threshold only when the rate correlation is very high. PMID:24550315

  8. Variable region gene analysis of B cell subsets derived from a 4-year- old child: somatically mutated memory B cells accumulate in the peripheral blood already at young age

    PubMed Central

    1994-01-01

    Tonsillar germinal center and immunoglobulin M+ (IgM+)IgD+ B cells as well as peripheral blood (PB) CD5+ and CD5- (conventional) B cells from a 4-yr-old child were isolated and nucleotide sequences of expressed Ig heavy chain variable regions encoded by VH4 gene family members were determined from amplified cDNA. Whereas both tonsillar IgM+IgD+ cells and the majority of IgM-expressing CD5+ and CD5- PB B cells showed no or little somatic mutation, tonsillar germinal center (GC) B cells and IgG-expressing PB B cells carried a high load of somatic mutations in their V region genes. This suggests that somatically mutated memory B cells which have switched isotype accumulate in the PB already at young age. Their frequency seems to increase with age. On the other hand, the antibody repertoire of tonsillar IgM+IgD+ B cells and the majority of IgM-expressing PB B cells is determined by germline-encoded specificities and by generation of variability in the complementary determining region III through VH-DH-JH recombination. A fraction of IgM-bearing PB B cells carries somatically mutated V region genes and probably represents GC-derived B cells which have left the GC at an early stage of the GC reaction without undergoing isotype switching. 10 VH4 germline genes were found to be expressed. Three gene segments were overrepresented in the sequence collection (35 of 50 clones): VH4.21 (30%), V71-4 (20%), and 3D279D (20%). It appears that most potentially functional VH4 germline genes are expressed in peripheral B cells. Some members of this VH gene family are clearly overrepresented over others. PMID:7931072

  9. Peak Satellite-to-Earth Data Rates Derived From Measurements of a 20 Gbps Bread-Board Modem

    NASA Technical Reports Server (NTRS)

    Landon, David G.; Simons, Rainee N.; Wintucky, Edwin G.; Sun, Jun Y.; Winn, James S.; Laraway, Stephen A.; McIntire, William K.; Metz, John L.; Smith, Francis J.

    2011-01-01

    A prototype data link using a Ka-band space qualified, high efficiency 200 W TWT amplifier and a bread-board modem emulator were created to explore the feasibility of very high speed communications in satellite-to-earth applications. Experiments were conducted using a DVB-S2-like waveform with modifications to support up to 20 Gbps through the addition of 128-Quadrature Amplitude Modulation (QAM). Limited by the bandwidth of the amplifier, a constant peak symbol rate of 3.2 Giga-symbols/sec was selected and the modulation order was varied to explore what peak data rate might be supported by an RF link through this amplifier. Using 128-QAM, an implementation loss of 3 dB was observed at 20 Gbps, and the loss decreased as data rate or bandwidth were reduced. Building on this measured data, realistic link budget calculations were completed. Low-Earth orbit (LEO) missions based on this TWTA with reasonable hardware assumptions and antenna sizing are found to be bandwidth-limited, rather than power-limited, making the spectral efficiency of 9/10-rate encoded 128-QAM very attractive. Assuming a bandwidth allocation of 1 GHz, these computations indicate that low-Earth orbit vehicles could achieve data rates up to 5 Gbps-an order of magnitude beyond the current state-of-practice, yet still within the processing power of a current FPGA-based software-defined modem. The measured performance results and a description of the experimental setup are presented to support these conclusions.

  10. Disparities between Phaeocystis in situ and optically-derived carbon biomass and growth rates: potential effect on remote-sensing primary production estimates

    NASA Astrophysics Data System (ADS)

    Peperzak, L.; van der Woerd, H. J.; Timmermans, K. R.

    2014-04-01

    The oceans play a pivotal role in the global carbon cycle. Unfortunately, the daily production of organic carbon, the product of phytoplankton standing stock and growth rate cannot be measured globally by discrete oceanographic methods. Instead, optical proxies from Earth-orbiting satellites must be used. To test the accuracy of optically-derived proxies of phytoplankton physiology and growth rate, standard ex situ data from the wax and wane of a Phaeocystis bloom in laboratory mesocosms were compared with hyperspectral reflectance data. Chlorophyll biomass could be estimated accurately from reflectance using specific chlorophyll absorption algorithms. However, the conversion of chlorophyll (Chl) to carbon (C) was obscured by the observed increase in C : Chl under nutrient-limited growth. C : Chl was inversely correlated (r2 = 0.88) with Photosystem II quantum efficiency (Fv/Fm), the in situ fluorometric oceanographic proxy for growth rate. In addition, the optical proxy for growth rate, the quantum efficiency of fluorescence ϕ was linearly correlated to Fv/Fm (r2 = 0.84), but not - as by definition - by using total phytoplankton absorption, because during nutrient-limited growth the concentrations of non-fluorescent light-absorbing pigments increased. As a consequence, none of the three proxies (C : Chl, Fv/Fm, φ) was correlated to carbon or cellular phytoplankton growth rates. Therefore, it is concluded that although satellite derived estimates of chlorophyll biomass may be accurate, physiologically-induced non-linear shifts in growth rate proxies may obscure accurate phytoplankton growth rates and hence global carbon production estimates.

  11. Investigating the past and recent δ18O-accumulation relationship seen in Greenland ice cores

    NASA Astrophysics Data System (ADS)

    Buchardt, S. L.; Clausen, H. B.; Vinther, B. M.; Dahl-Jensen, D.

    2012-12-01

    Decadal means of δ18O and accumulation rates from 52 ice core locations in Greenland are presented. The accumulation rates are derived from annual layers determined in the δ18O curve. Investigation of the δ18O-accumulation relationship across the ice divide reveals a significant Foehn effect with anticorrelation of δ18O and accumulation rate on the lee side of the divide in Southern Greenland, while no effect is seen in Central Greenland. Furthermore, the sensitivity of accumulation rate to changes in temperature is found to be smaller in Northern Greenland than in the central and southern parts. Four records in the data set contain sufficient recent data that the period of observed temperature rise from the 1990s and onwards can be investigated. All four records are from locations close to the ice divide in Northern Greenland and while three of them show increased temperatures, no conclusive statement can be made about the accumulation rate from these data.

  12. Organic-Carbon Sequestration in Soil/Sediment of the Mississippi River Deltaic Plain - Data; Landscape Distribution, Storage, and Inventory; Accumulation Rates; and Recent Loss, Including a Post-Katrina Preliminary Analysis (Chapter B)

    USGS Publications Warehouse

    Markewich, Helaine W.; Buell, Gary R.; Britsch, Louis D.; McGeehin, John P.; Robbins, John A.; Wrenn, John H.; Dillon, Douglas L.; Fries, Terry L.; Morehead, Nancy R.

    2007-01-01

    Soil/sediment of the Mississippi River deltaic plain (MRDP) in southeastern Louisiana is rich in organic carbon (OC). The MRDP contains about 2 percent of all OC in the surface meter of soil/sediment in the Mississippi River Basin (MRB). Environments within the MRDP differ in soil/sediment organic carbon (SOC) accumulation rate, storage, and inventory. The focus of this study was twofold: (1) develop a database for OC and bulk density for MRDP soil/sediment; and (2) estimate SOC storage, inventory, and accumulation rates for the dominant environments (brackish, intermediate, and fresh marsh; natural levee; distributary; backswamp; and swamp) in the MRDP. Comparative studies were conducted to determine which field and laboratory methods result in the most accurate and reproducible bulk-density values for each marsh environment. Sampling methods included push-core, vibracore, peat borer, and Hargis1 sampler. Bulk-density data for cores taken by the 'short push-core method' proved to be more internally consistent than data for samples collected by other methods. Laboratory methods to estimate OC concentration and inorganic-constituent concentration included mass spectrometry, coulometry, and loss-on-ignition. For the sampled MRDP environments, these methods were comparable. SOC storage was calculated for each core with adequate OC and bulk-density data. SOC inventory was calculated using core-specific data from this study and available published and unpublished pedon data linked to SSURGO2 map units. Sample age was estimated using isotopic cesium (137Cs), lead (210Pb), and carbon (14C), elemental Pb, palynomorphs, other stratigraphic markers, and written history. SOC accumulation rates were estimated for each core with adequate age data. Cesium-137 profiles for marsh soil/sediment are the least ambiguous. Levee and distributary 137Cs profiles show the effects of intermittent allochthonous input and/or sediment resuspension. Cesium-137 and 210Pb data gave the most

  13. Deriving fractional rate of degradation of logistic-exponential (LE) model to evaluate early in vitro fermentation.

    PubMed

    Wang, M; Sun, X Z; Tang, S X; Tan, Z L; Pacheco, D

    2013-06-01

    Water-soluble components of feedstuffs are mainly utilized during the early phase of microbial fermentation, which could be deemed an important determinant of gas production behavior in vitro. Many studies proposed that the fractional rate of degradation (FRD) estimated by fitting gas production curves to mathematical models might be used to characterize the early incubation for in vitro systems. In this study, the mathematical concept of FRD was developed on the basis of the Logistic-Exponential (LE) model, with initial gas volume being zero (LE0). The FRD of the LE0 model exhibits a continuous increase from initial (FRD 0) toward final asymptotic value (FRD F) with longer incubation time. The relationships between the FRD and gas production at incubation times 2, 4, 6, 8, 12 and 24 h were compared for four models, in addition to LE0, Generalization of the Mitscherlich (GM), c th order Michaelis-Menten (MM) and Exponential with a discrete LAG (EXPLAG). A total of 94 in vitro gas curves from four subsets with a wide range of feedstuffs from different laboratories and incubation periods were used for model testing. Results indicated that compared with the GM, MM and EXPLAG models, the FRD of LE0 model consistently had stronger correlations with gas production across the four subsets, especially at incubation times 2, 4, 6, 8 and 12 h. Thus, the LE0 model was deemed to provide a better representation of the early fermentation rates. Furthermore, the FRD 0 also exhibited strong correlations (P < 0.05) with gas production at early incubation times 2, 4, 6 and 8 h across all four subsets. In summary, the FRD of LE0 model provides an alternative to quantify the rate of early stage incubation, and its initial value could be an important starting parameter of rate.

  14. Analysis of SFMR-Derived and Satellite-Based Rain Rates over the Tropical Western North Pacific

    DTIC Science & Technology

    2010-03-01

    Figure 5. A TRMM TMI and PR rain rate (mm h-1) pass over TY Sinlaku at 1947 UTC 12 Sep 2008. Note the wider swath is for...the TMI and the smaller swath is for the PR data (Image available from http://www.nrlmry.navy.mil/TC.html 12...observations. These platforms have been operational for many years and newer technologies have been built upon algorithms and research that have

  15. 10Be-derived denudation rates from the Burdekin catchment: The largest contributor of sediment to the Great Barrier Reef

    NASA Astrophysics Data System (ADS)

    Croke, Jacky; Bartley, Rebecca; Chappell, John; Austin, Jenet M.; Fifield, Keith; Tims, Stephen G.; Thompson, Chris J.; Furuichi, Takahisa

    2015-07-01

    Terrestrial cosmogenic nuclides (TCNs) such as Beryllium-10 (10Be) are now routinely used to reconstruct erosional rates over tens of thousands of years at increasingly large basin scales (> 100,000 km2). In Australia, however, the approach and its assumptions have not been systematically tested within a single, large drainage basin. This study measures 10Be concentrations in river sediments from the Burdekin catchment, one of Australia's largest coastal catchments, to determine long-term (> 10,000 years), time-integrated rates of sediment generation and denudation. A nested-sampling design was used to test for effects of increasing catchment scale on nuclide concentrations with upstream catchment areas ranging from 4 to 130,000 km2. Beryllium-10 concentrations in sediment samples collected from the upstream headwater tributaries and mid-stream locations range from 1.8 to 2.89 × 105 atoms g- 1 and data confirm that nuclide concentrations are well and rapidly mixed downstream. Sediment from the same tributaries consistently yielded 10Be concentrations in the range of their upstream samples. Overall, no decrease in 10Be concentrations can be observed at the range of catchment scales measured here. The mean denudation rate for all river sediment samples throughout the Fanning subcatchment (1100 km2) is 18.47 m Ma- 1, which compares with the estimate at the end of the Burdekin catchment (130,000 km2) of 16.22 m Ma- 1. Nuclide concentrations in the lower gradient western and southern catchments show a higher degree of variability, and several complications emerged as a result of the contrasting geomorphic processes and settings. This study confirms the ability of TCNs to determine long-term denudation rates in Australia and highlights some important considerations in the model assumptions that may affect the accuracy of limited sampling in large, low-gradient catchments with long storage times.

  16. Lithosphere thickness and mantle viscosity estimated from joint inversion of GPS and GRACE-derived radial deformation and gravity rates in North America

    NASA Astrophysics Data System (ADS)

    Zhao, S.

    2013-09-01

    The Global Positioning System (GPS) and the Gravity Recovery and Climate Experiment (GRACE) have been used to respectively determine the Earth's surface deformation and gravity changes associated with glacial isostatic adjustment, which is caused by ongoing stress release of the viscoelastic mantle after removal of the Late Pleistocene ice sheets. Here we present a joint inversion analysis of GPS-derived radial (vertical) deformation and GRACE-derived gravity rates in North America to examine whether the ice sheets (ICE-3G and ICE-5G) and earth models can fit the satellite based observations. The results of joint inversion give an effective lithosphere thickness of 150 km (110-180 km under a statistical confidence level of 80 per cent), an upper-mantle viscosity of 3.7 (2.0-5.0; 90 per cent confidence level) × 1020 Pa s, and a lower-mantle viscosity of 1.9 (1.3-2.6; 90 per cent confidence level) × 1021 Pa s. More sophisticated models such as introducing a transition zone of 400-670 km are not fully resolved with current data sets because there is no significant improvement in fitting observations. Tests of modifying ICE-5G show that a reduction of ice thickness by ˜20 per cent in the area west of Hudson Bay and an increase by ˜40 per cent in the southeast (Quebec region) are required to fit both observed vertical deformation and gravity changes. An additional test from inversion analysis of GRACE-derived geoid rates confirms possible signal loss in the GRACE-derived gravity rates, which could be due to noise reduction methods used in data processing stages.

  17. Study of H/D exchange rates to derive the strength of intramolecular hydrogen bonds in halo substituted organic building blocks: An NMR spectroscopic investigation

    NASA Astrophysics Data System (ADS)

    Mishra, Sandeep Kumar; Suryaprakash, N.

    2015-10-01

    Rates of hydrogen/deuterium (H/D) exchange determined by 1H NMR spectroscopy are utilized to derive the strength of hydrogen bonds and to monitor the electronic effects in the site-specific halogen substituted benzamides and anilines. The theoretical fitting of the time dependent variation of the integral areas of 1H NMR resonances to the first order decay function permitted the determination of H/D exchange rate constants (k) and their precise half-lives (t1/2) with high degree of reproducibility. The comparative study also permitted the unambiguous determination of relative strength of hydrogen bonds and the contribution from electronic effects on the H/D exchange rate.

  18. Derivation of a formula describing the saturation correction of plane-parallel ionization chambers in pulsed fields with arbitrary repetition rate.

    PubMed

    Karsch, Leonhard

    2016-04-21

    Gas-filled ionization chambers are widely used radiation detectors in radiotherapy. A quantitative description and correction of the recombination effects exists for two cases, for continuous radiation exposure and for pulsed radiation fields with short single pulses. This work gives a derivation of a formula for pulsed beams with arbitrary pulse rate for which the prerequisites of the two existing descriptions are not fulfilled. Furthermore, an extension of the validity of the two known cases is investigated. The temporal evolution of idealized charge density distributions within a plane parallel chamber volume is described for pulsed beams of vanishing pulse duration and arbitrary pulse repetition rate. First, the radiation induced release, movement and collection of the charge carriers without recombination are considered. Then, charge recombination is calculated basing on these simplified charge distributions and the time dependent spatial overlap of positive and negative charge carrier distributions. Finally, a formula for the calculation of the saturation correction factor is derived by calculation and simplification of the first two terms of a Taylor expansion for small recombination. The new formula of saturation correction contains the two existing cases, descriptions for exposure by single pulses and continuous irradiation, as limiting cases. Furthermore, it is possible to determine the pulse rate range for which each of the three descriptions is applicable by comparing the dependencies of the new formula with the two existing cases. As long as the time between two pulses is lower than one third of the collection time of the chamber, the formalism for a continuous exposure can be used. The known description for single pulse irradiation is only valid if the repetition rate is less than 1.2 times the inverse collection time. For all other repetition rates in between the new formula should be used. The experimental determination by Jaffe diagrams can be

  19. The relationship between oxygen consumption rate and viability of in vivo-derived pig embryos vitrified by the micro volume air cooling method.

    PubMed

    Sakagami, N; Nishida, K; Misumi, K; Hirayama, Y; Yamashita, S; Hoshi, H; Misawa, H; Akiyama, K; Suzuki, C; Yoshioka, K

    2016-01-01

    The aim of this study was to assess the viability of vitrified-warmed in vivo-derived pig embryos after measuring the oxygen consumption rate. Six days after artificial insemination, blastocysts were collected from gilts and vitrified by the micro volume air cooling method. The oxygen consumption rate was measured in 60 vitrified-warmed embryos, which were then cultured for 48h to assess the viability. The survival (re-expansion) rate of embryos after warming was 85.0%. The average oxygen consumption rate of embryos immediately after warming was greater in embryos which could re-expand during subsequent culture (F=0.75±0.04) than that in those which failed to re-expand (F=0.33±0.05). Moreover, the oxygen consumption rate of vitrified-warmed embryos was greater in the hatched (F=0.88±0.06) than that in the not-hatched group (F=0.53±0.04). When the oxygen consumption rate of the vitrified-warmed embryos and the numbers of viable and dead cells in embryos were determined, there was a positive correlation between the oxygen consumption rate and the number of live cells (P<0.01, r=0.538). A total of 29 vitrified embryos after warming and measuring the oxygen consumption rate were surgically transferred into uterine horns of two recipients. Both of the recipients become pregnant and farrowed 12 healthy piglets. These results demonstrate that the oxygen consumption rate of vitrified-warmed pig embryos can be related to the number of live cells and that the measurement of oxygen consumption of embryos after cryopreservation may be useful for estimating embryo survivability.

  20. Field metabolic rate and PCB adipose tissue deposition efficiency in East Greenland polar bears derived from contaminant monitoring data.

    PubMed

    Pavlova, Viola; Nabe-Nielsen, Jacob; Dietz, Rune; Svenning, Jens-Christian; Vorkamp, Katrin; Rigét, Frank Farsø; Sonne, Christian; Letcher, Robert J; Grimm, Volker

    2014-01-01

    Climate change will increasingly affect the natural habitat and diet of polar bears (Ursus maritimus). Understanding the energetic needs of polar bears is therefore important. We developed a theoretical method for estimating polar bear food consumption based on using the highly recalcitrant polychlorinated biphenyl (PCB) congener, 2,2',4,4',55-hexaCB (CB153) in bear adipose tissue as an indicator of food intake. By comparing the CB153 tissue concentrations in wild polar bears with estimates from a purposely designed individual-based model, we identified the possible combinations of field metabolic rates (FMR) and CB153 deposition efficiencies in East Greenland polar bears. Our simulations indicate that if 30% of the CB153 consumed by polar bear individuals were deposited into their adipose tissue, the corresponding FMR would be only two times the basal metabolic rate. In contrast, if the modelled CB153 deposition efficiency were 10%, adult polar bears would require six times more energy than that needed to cover basal metabolism. This is considerably higher than what has been assumed for polar bears in previous studies though it is similar to FMRs found in other marine mammals. An implication of this result is that even relatively small reductions in future feeding opportunities could impact the survival of East Greenland polar bears.

  1. Field Metabolic Rate and PCB Adipose Tissue Deposition Efficiency in East Greenland Polar Bears Derived from Contaminant Monitoring Data

    PubMed Central

    Pavlova, Viola; Nabe-Nielsen, Jacob; Dietz, Rune; Svenning, Jens-Christian; Vorkamp, Katrin; Rigét, Frank Farsø; Sonne, Christian; Letcher, Robert J.; Grimm, Volker

    2014-01-01

    Climate change will increasingly affect the natural habitat and diet of polar bears (Ursus maritimus). Understanding the energetic needs of polar bears is therefore important. We developed a theoretical method for estimating polar bear food consumption based on using the highly recalcitrant polychlorinated biphenyl (PCB) congener, 2,2′,4,4′,55-hexaCB (CB153) in bear adipose tissue as an indicator of food intake. By comparing the CB153 tissue concentrations in wild polar bears with estimates from a purposely designed individual-based model, we identified the possible combinations of field metabolic rates (FMR) and CB153 deposition efficiencies in East Greenland polar bears. Our simulations indicate that if 30% of the CB153 consumed by polar bear individuals were deposited into their adipose tissue, the corresponding FMR would be only two times the basal metabolic rate. In contrast, if the modelled CB153 deposition efficiency were 10%, adult polar bears would require six times more energy than that needed to cover basal metabolism. This is considerably higher than what has been assumed for polar bears in previous studies though it is similar to FMRs found in other marine mammals. An implication of this result is that even relatively small reductions in future feeding opportunities could impact the survival of East Greenland polar bears. PMID:25101837

  2. K(+) accumulation in the cytoplasm and nucleus of the salt gland cells of Limonium bicolor accompanies increased rates of salt secretion under NaCl treatment using NanoSIMS.

    PubMed

    Feng, Zhong-Tao; Deng, Yun-Quan; Zhang, Shi-Chao; Liang, Xue; Yuan, Fang; Hao, Jia-Long; Zhang, Jian-Chao; Sun, Shu-Feng; Wang, Bao-Shan

    2015-09-01

    Recretohalophytes with specialized salt-secreting structures (salt glands) can secrete excess salts from plant, while discriminating between Na(+) and K(+). K(+)/Na(+) ratio plays an important role in plant salt tolerance, but the distribution and role of K(+) in the salt gland cells is poorly understood. In this article, the in situ subcellular localization of K and Na in the salt gland of the recretohalophyte Limonium bicolor Kuntze is described. Samples were prepared by high-pressure freezing (HPF), freeze substitution (FS) and analyzed using NanoSIMS. The salt gland of L. bicolor consists of sixteen cells. Higher signal strength of Na(+) was located in the apoplast of salt gland cells. Compared with control, 200 mM NaCl treatment led to higher signal strength of K(+) and Na(+) in both cytoplasm and nucleus of salt gland cells although K(+)/Na(+) ratio in both cytoplasm and nucleus were slightly reduced by NaCl. Moreover, the rate of Na(+) secretion per salt gland of L. bicolor treated with 200 mM NaCl was five times that of controls. These results suggest that K(+) accumulation both in the cytoplasm and nucleus of salt gland cells under salinity may play an important role in salt secretion, although the exact mechanism is unknown.

  3. Comparative survival rates of human-derived probiotic Lactobacillus paracasei and L. salivarius strains during heat treatment and spray drying.

    PubMed

    Gardiner, G E; O'Sullivan, E; Kelly, J; Auty, M A; Fitzgerald, G F; Collins, J K; Ross, R P; Stanton, C

    2000-06-01

    Spray drying of skim milk was evaluated as a means of preserving Lactobacillus paracasei NFBC 338 and Lactobacillus salivarius UCC 118, which are human-derived strains with probiotic potential. Our initial experiments revealed that NFBC 338 is considerably more heat resistant in 20% (wt/vol) skim milk than UCC 118 is; the comparable decimal reduction times were 11.1 and 1.1 min, respectively, at 59 degrees C. An air outlet temperature of 80 to 85 degrees C was optimal for spray drying; these conditions resulted in powders with moisture contents of 4.1 to 4.2% and viable counts of 3.2 x 10(9) CFU/g for NFBC 338 and 5.2 x 10(7) CFU/g for UCC 118. Thus, L. paracasei NFBC 338 survived better than L. salivarius UCC 118 during spray drying; similar results were obtained when we used confocal scanning laser microscopy and LIVE/DEAD BacLight viability staining. In addition, confocal scanning laser microscopy revealed that the probiotic lactobacilli were located primarily in the powder particles. Although both spray-dried cultures appeared to be stressed, as shown by increased sensitivity to NaCl, bacteriocin production by UCC 118 was not affected by the process, nor was the activity of the bacteriocin peptide. The level of survival of NFBC 338 remained constant at approximately 1 x 10(9) CFU/g during 2 months of powder storage at 4 degrees C, while a decline in the level of survival of approximately 1 log (from 7.2 x 10(7) to 9.5 x 10(6) CFU/g) was observed for UCC 118 stored under the same conditions. However, survival of both Lactobacillus strains during powder storage was inversely related to the storage temperature. Our data demonstrate that spray drying may be a cost-effective way to produce large quantities of some probiotic cultures.

  4. Comparative Survival Rates of Human-Derived Probiotic Lactobacillus paracasei and L. salivarius Strains during Heat Treatment and Spray Drying

    PubMed Central

    Gardiner, G. E.; O'Sullivan, E.; Kelly, J.; Auty, M. A. E.; Fitzgerald, G. F.; Collins, J. K.; Ross, R. P.; Stanton, C.

    2000-01-01

    Spray drying of skim milk was evaluated as a means of preserving Lactobacillus paracasei NFBC 338 and Lactobacillus salivarius UCC 118, which are human-derived strains with probiotic potential. Our initial experiments revealed that NFBC 338 is considerably more heat resistant in 20% (wt/vol) skim milk than UCC 118 is; the comparable decimal reduction times were 11.1 and 1.1 min, respectively, at 59°C. An air outlet temperature of 80 to 85°C was optimal for spray drying; these conditions resulted in powders with moisture contents of 4.1 to 4.2% and viable counts of 3.2 × 109 CFU/g for NFBC 338 and 5.2 × 107 CFU/g for UCC 118. Thus, L. paracasei NFBC 338 survived better than L. salivarius UCC 118 during spray drying; similar results were obtained when we used confocal scanning laser microscopy and LIVE/DEAD BacLight viability staining. In addition, confocal scanning laser microscopy revealed that the probiotic lactobacilli were located primarily in the powder particles. Although both spray-dried cultures appeared to be stressed, as shown by increased sensitivity to NaCl, bacteriocin production by UCC 118 was not affected by the process, nor was the activity of the bacteriocin peptide. The level of survival of NFBC 338 remained constant at ∼1 × 109 CFU/g during 2 months of powder storage at 4°C, while a decline in the level of survival of approximately 1 log (from 7.2 × 107 to 9.5 × 106 CFU/g) was observed for UCC 118 stored under the same conditions. However, survival of both Lactobacillus strains during powder storage was inversely related to the storage temperature. Our data demonstrate that spray drying may be a cost-effective way to produce large quantities of some probiotic cultures. PMID:10831444

  5. Rationally and empirically derived dimensions of children's symptomatology: expert ratings and confirmatory factor analyses of the CBCL.

    PubMed

    Lengua, L J; Sadowski, C A; Friedrich, W N; Fisher, J

    2001-08-01

    Expert ratings and confirmatory factor analyses were used to develop an alternative system for scoring the Child Behavior Checklist (CBCL; T. M. Achenbach, 1991) to measure specific dimensions corresponding to current conceptualizations of child symptomatology. Data were from a nonclinic and 2 independent clinic samples. Subscales measuring Anxiety, Attention Problems/Hyperactivity, Conduct Problems, Depression, Oppositional Defiant, Social Problems/Immaturity, and Somatization were created. Logistic regressions were conducted to evaluate the diagnostic efficiency and discrimination of the new and original approaches to scoring the CBCL. Some of the new subscales demonstrated better sensitivity, positive predictive power, and discriminant validity than the original CBCL subscales; however, subscales from both approaches demonstrated low sensitivity. Results support the use of the new subscales for specific research purposes.

  6. Airborne-radar and ice-core observations of snow accumulation in West Antarctica

    NASA Astrophysics Data System (ADS)

    Medley, Brooke

    The world's ice sheets store enough water to raise global eustatic sea level by several tens of meters, and therefore, any fluctuations in their size will cause sea level to rise or fall. The net mass exchanged with the ocean - defined as the mass balance - determines the glacial contribution to sea level and is the difference in snow accumulated in the interior and ice discharged into the ocean at the ice sheet periphery. While new techniques in remotely acquired surface velocities lead to improved discharge measurements, snow accumulation remains unmeasured over much of the of the ice sheet. This work aims to improve our understanding of snow accumulation over two of the most rapidly evolving glaciers in Antarctica: Pine Island and Thwaites. Specifically, we use two airborne radar systems to image and track the near-surface internal stratigraphy to measure snow accumulation rates over both glaciers. This method allows for investigation of the spatial and temporal variations in accumulation at the catchment-scale, which is essential for determining glacier mass balance. Examination of the radar-derived accumulation rates over Pine Island and Thwaites glaciers revealed several results including: (1) accumulation exhibited no significant trend between 1980 and 2009, (2) the sea-level contribution from Pine Island and Thwaites tripled from +0.09 mm yr-1 in the mid-1990s to +0.27 mm yr-1 by 2010, (3) a shift towards higher accumulation occurred between 1944-1984 and 1985-2009, observed in both ice core and radar records, and (4) atmospheric models are an adequate replacement for accumulation measurements in areas with few observations. These findings indicate that accumulation is not concurrently compensating the enhanced ice discharge from the region, and as a result, the sea-level contribution from these glaciers is increasing. Furthermore, a recent shift towards higher mean accumulation suggests these glaciers might have been out of balance earlier than originally

  7. Laboratory-derived temperature preference and effect on the feeding rate and survival of juvenile Hemimysis anomala

    USGS Publications Warehouse

    Sun, Jennifer; Rudstam, Lars S.; Boscarino, Brent T.; Walsh, Maureen G.; Lantry, Brian F.

    2013-01-01

    Hemimysis anomala is a warm-water mysid that invaded the Great Lakes region in 2006 and has since rapidly spread throughout the basin. We conducted three laboratory experiments to better define the temperature preference, tolerance limits, and temperature effects on feeding rates of juvenile Hemimysis, using individuals acclimated to mid (16 °C) and upper (22 °C) preferred temperature values previously reported for the species. For temperature preference, we fit a two-parameter Gaussian (μ, σ) function to the experimental data, and found that the peak values (μ, interpreted as the preference temperature) were 22.0 °C (SE 0.25) when acclimated to 16 and 21.9 °C (SE 0.38) when acclimated to 22 °C, with the σ-values of the curves at 2.6 and 2.5 °C, respectively. No mysids were observed in temperatures below 10 or above 28 °C in these preference experiments. In short-term tolerance experiments for temperatures between 4 and 32 °C, all mysids died within 8 h at 30.2 °C for 16 °C acclimated mysids, and at 31.8 °C for 22 °C acclimated mysids. No lower lethal limit was found. Feeding rates increased with temperature from an average of 4 Bosmina eaten per hour at 5 °C to 19 Bosmina eaten per hour at 27 °C. The results of our experiments indicate an optimal temperature for Hemimysis between 21 and 27 °C, which corresponds with temperatures during periods of high population growth in the field. These results contribute a better understanding of this species' biological response to temperature that will help guide field studies and inform bioenergetics modeling.

  8. Cysteamine supplementation during in vitro maturation of slaughterhouse- and opu-derived bovine oocytes improves embryonic development without affecting cryotolerance, pregnancy rate, and calf characteristics.

    PubMed

    Merton, J S; Knijn, H M; Flapper, H; Dotinga, F; Roelen, B A J; Vos, P L A M; Mullaart, E

    2013-09-01

    Optimization of ovum pick up (OPU) followed by in vitro embryo production (IVP) is strongly driven by the needs of both beef and dairy cattle breeders to enhance genetic improvement. The rapidly growing use of genomic selection in cattle has increased the interest in using OPU-IVP technology to increase the number of embryos and offspring per donor, thus allowing enhanced selection intensity for the next generation. The aim of this study was to optimize embryo production through supplementation of cysteamine during in vitro maturation (IVM) and in vitro culture (IVC) of both slaughterhouse- and OPU-derived oocytes. The effects on embryo production and on embryo cryotolerance, post-transfer embryo survival, and calf characteristics, including gestation length, birth weight, perinatal mortality, and sex ratio were studied. In study 1, immature slaughterhouse-derived cumulus-oocyte complexes (COCs) were matured in IVM medium supplemented with or without 0.1 mM cysteamine, fertilized and cultured for 7 days in 0.5 ml SOFaaBSA. In study 2, cysteamine was present during both IVM (0.1 mM) and IVC (0.01, 0.05, 0.1 mM) from Days 1 to 4. In study 3, OPU-derived COCs were matured in medium supplemented with or without 0.1 mM cysteamine in a 2 × 2 factorial design (OPU week and cysteamine treatment). Embryos were evaluated for stage and grade on Day 7 and, depending on the number of transferable embryos and recipients available, the embryos were transferred either fresh or frozen-thawed at a later date. The presence of cysteamine during IVM significantly increased the embryo production rate with slaughterhouse-derived COCs (24.0% vs. 19.4%). The higher number of embryos at Day 7 was due to an increased number of blastocysts, whereas the distribution of embryos among different quality grades and cryotolerance was not affected. Embryo production rate was negatively affected when cysteamine was present during both the processes of IVM and IVC during Days 1 to 4 of culture (13

  9. An alternative derivation of the stationary distribution of the multivariate neutral Wright-Fisher model for low mutation rates with a view to mutation rate estimation from site frequency data.

    PubMed

    Schrempf, Dominik; Hobolth, Asger

    2017-04-01

    Recently, Burden and Tang (2016) provided an analytical expression for the stationary distribution of the multivariate neutral Wright-Fisher model with low mutation rates. In this paper we present a simple, alternative derivation that illustrates the approximation. Our proof is based on the discrete multivariate boundary mutation model which has three key ingredients. First, the decoupled Moran model is used to describe genetic drift. Second, low mutation rates are assumed by limiting mutations to monomorphic states. Third, the mutation rate matrix is separated into a time-reversible part and a flux part, as suggested by Burden and Tang (2016). An application of our result to data from several great apes reveals that the assumption of stationarity may be inadequate or that other evolutionary forces like selection or biased gene conversion are acting. Furthermore we find that the model with a reversible mutation rate matrix provides a reasonably good fit to the data compared to the one with a non-reversible mutation rate matrix.

  10. Infection Rate and Tissue Localization of Murine IL-12p40-Producing Monocyte-Derived CD103+ Lung Dendritic Cells during Pulmonary Tuberculosis

    PubMed Central

    Leepiyasakulchai, Chaniya; Taher, Chato; Chuquimia, Olga D.; Mazurek, Jolanta; Söderberg-Naucler, Cecilia; Fernández, Carmen; Sköld, Markus

    2013-01-01

    Non-hematopoietic cells, including lung epithelial cells, influence host immune responses. By co-culturing primary alveolar epithelial cells and monocytes from naïve donor mice, we show that alveolar epithelial cells support monocyte survival and differentiation in vitro, suggesting a role for non-hematopoietic cells in monocyte differentiation during the steady state in vivo. CD103+ dendritic cells (αE-DC) are present at mucosal surfaces. Using a murine primary monocyte adoptive transfer model, we demonstrate that αE-DC in the lungs and pulmonary lymph nodes are monocyte-derived during pulmonary tuberculosis. The tissue localization may influence the functional potential of αE-DC that accumulate in Mycobacterium tuberculosis-infected lungs. Here, we confirm the localization of αE-DC in uninfected mice beneath the bronchial epithelial cell layer and near the vascular wall, and show that αE-DC have a similar distribution in the lungs during pulmonary tuberculosis and are detected in the bronchoalveolar lavage fluid from infected mice. Lung DC can be targeted by M. tuberculosis in vivo and play a role in bacterial dissemination to the draining lymph node. In contrast to other DC subsets, only a fraction of lung αE-DC are infected with the bacterium. We also show that virulent M. tuberculosis does not significantly alter cell surface expression levels of MHC class II on infected cells in vivo and that αE-DC contain the highest frequency of IL-12p40+ cells among the myeloid cell subsets in infected lungs. Our results support a model in which inflammatory monocytes are recruited into the M. tuberculosis-infected lung tissue and, depending on which non-hematopoietic cells they interact with, differentiate along different paths to give rise to multiple monocyte-derived cells, including DC with a distinctive αE-DC phenotype. PMID:23861965

  11. Prediction models of human plasma protein binding rate and oral bioavailability derived by using GA-CG-SVM method.

    PubMed

    Ma, Chang-Ying; Yang, Sheng-Yong; Zhang, Hui; Xiang, Ming-Li; Huang, Qi; Wei, Yu-Quan

    2008-08-05

    In this study, support vector machine (SVM) method combined with genetic algorithm (GA) for feature selection and conjugate gradient (CG) method for parameter optimization (GA-CG-SVM), has been employed to develop prediction models of human plasma protein binding rate (PPBR) and oral bioavailability (BIO). The advantage of the GA-CG-SVM is that it can deal with feature selection and SVM parameter optimization simultaneously. Five-fold cross-validation as well as independent test set method were used to validate the prediction models. For the PPBR, a total of 692 compounds were used to train and test the prediction model. The prediction accuracy by means of 5-fold cross-validation is 86% and that for the independent test set (161 compounds) is 81%. These accuracies are markedly higher over that of the best model currently available in literature. The number of descriptors selected is 29. For the BIO, the training set is composed of 690 compounds and external 76 compounds form an independent validation set. The prediction accuracy for the training set by using 5-fold cross-validation and that for the independent test set are 80% and 86%, respectively, which are better than or comparable to those of other classification models in literature. The number of descriptors selected is 25. For both the PPBR and BIO, the descriptors selected by GA-CG method cover a large range of molecular properties which imply that the PPBR and BIO of a drug might be affected by many complicated factors.

  12. Ethambutol-mediated cell wall modification in recombinant Corynebacterium glutamicum increases the biotransformation rates of cyclohexanone derivatives.

    PubMed

    Yun, Ji-Yeong; Lee, Jung-Eun; Yang, Kyung-Mi; Cho, Suekyung; Kim, Arim; Kwon, Yong-Uk; Kwon, Yong-Euk; Park, Jin-Byung

    2012-01-01

    The effects of structural modification of cell wall on the biotransformation capability by recombinant Corynebacterium glutamicum cells, expressing the chnB gene encoding cyclohexanone monooxygenase of Acinetobacter calcoaceticus NCIMB 9871, were investigated. Baeyer-Villiger oxygenation of 2-(2'-acetoxyethyl) cyclohexanone (MW 170 Da) into R-7-(2'-acetoxyethyl)-2-oxepanone was used as a model reaction. The whole-cell biotransformation followed Michaelis-Menten kinetics. The V (max) and K (S) values were estimated as 96.8 U g(-1) of dry cells and 0.98 mM, respectively. The V (max) was comparable with that of cyclohexanone oxygenation, whereas the K (S) was almost eightfold higher. The K (S) value of 2-(2'-acetoxyethyl) cyclohexanone oxygenation was reduced by ca. 30% via altering the cell envelop structure of C. glutamicum with ethambutol, which inhibits arabinosyl transferases involved in the biosynthesis of cell wall arabinogalactan and mycolate layers. The higher whole-cell biotransformation rate was also observed in the oxygenation of ethyl 2-cyclohexanone acetate upon ethambutol treatment of the recombinant C. glutamicum. Therefore, it was assumed that the biotransformation efficiency of C. glutamicum-based biocatalysts, with respect to medium- to large-sized lipophilic organic substrates (MW > ca. 170), can be enhanced by engineering their cell wall outer layers, which are known to function as a formidable barrier to lipophilic molecules.

  13. Communication: Charge-transfer rate constants in zinc-porphyrin-porphyrin-derived dyads: a Fermi golden rule first-principles-based study.

    PubMed

    Manna, Arun K; Dunietz, Barry D

    2014-09-28

    We investigate photoinduced charge transfer (CT) processes within dyads consisting of porphyrin derivatives in which one ring ligates a Zn metal center and where the rings vary by their degree of conjugation. Using a first-principles approach, we show that molecular-scale means can tune CT rates through stabilization affected by the polar environment. Such means of CT tuning are important for achieving high efficiency optoelectronic applications using organic semiconducting materials. Our fully quantum mechanical scheme is necessary for reliably modeling the CT process across different regimes, in contrast to the pervading semi-classical Marcus picture that grossly underestimates transfer in the far-inverted regime.

  14. Rate of lateral magma transport in the Earth's crust beneath submarine volcanic arcs derived from earthquake swarm analysis

    NASA Astrophysics Data System (ADS)

    Spicak, A.; Vanek, J.

    2015-12-01

    This contribution deals with subduction-related submarine magmatism. We are offering a tool to contribute to delimitation of domains of current magma unrest at convergent plate margins and to understand better the behavior of magma in the lithospheric wedge above the subducting slab: a detailed analysis of teleseismic earthquake occurrence. A specific seismicity pattern has been observed beneath submarine portions of several volcanic arcs at convergent plate margins (Andaman Sea region, southern Ryukyu area). We have found three arguments that allowed us to interpret such a seismicity pattern as a magma-driven process: (i) clustering of medium-size earthquakes (M~5) in space and time in shallow earthquake swarms; (ii) rapid migration of seismic activity during the swarms (comparison of epicentral maps of individual stages of the swarm development showed consistently that earthquake epicenters migrate laterally at a rate of several hundred meters per hour); (iii) correlation of epicentral zones of the swarms with distinct seamounts and submarine ridges (current seismically active intrusions probably propagate along plumbing systems that served as conduits of magma to the surface in the past). The repeated occurrence of relatively strong, teleseismically recorded earthquake swarms thus probably reflects fluid and/or magma ascent through the plumbing system of the volcanic arc, points to brittle character of the lithospheric wedge at respective depths and favors the studied areas - the Andaman Sea region and the southern Ryukyu area - to be potential sites of submarine volcanic activity.. The study documents high accuracy of hypocenter parameter determinations published by data centers such as ISC and NEIC USGS, and the usefulness of the EHB relocation procedure.

  15. Normative evidence accumulation in unpredictable environments

    PubMed Central

    Glaze, Christopher M; Kable, Joseph W; Gold, Joshua I

    2015-01-01

    In our dynamic world, decisions about noisy stimuli can require temporal accumulation of evidence to identify steady signals, differentiation to detect unpredictable changes in those signals, or both. Normative models can account for learning in these environments but have not yet been applied to faster decision processes. We present a novel, normative formulation of adaptive learning models that forms decisions by acting as a leaky accumulator with non-absorbing bounds. These dynamics, derived for both discrete and continuous cases, depend on the expected rate of change of the statistics of the evidence and balance signal identification and change detection. We found that, for two different tasks, human subjects learned these expectations, albeit imperfectly, then used them to make decisions in accordance with the normative model. The results represent a unified, empirically supported account of decision-making in unpredictable environments that provides new insights into the expectation-driven dynamics of the underlying neural signals. DOI: http://dx.doi.org/10.7554/eLife.08825.001 PMID:26322383

  16. Derivation of a true (t → 0+) quantum transition-state theory. II. Recovery of the exact quantum rate in the absence of recrossing.

    PubMed

    Althorpe, Stuart C; Hele, Timothy J H

    2013-08-28

    In Paper I [T. J. H. Hele and S. C. Althorpe, J. Chem. Phys. 138, 084108 (2013)] we derived a quantum transition-state theory (TST) by taking the t → 0+ limit of a new form of quantum flux-side time-correlation function containing a ring-polymer dividing surface. This t → 0+ limit appears to be unique in giving positive-definite Boltzmann statistics, and is identical to ring-polymer molecular dynamics (RPMD) TST. Here, we show that quantum TST (i.e., RPMD-TST) is exact if there is no recrossing (by the real-time quantum dynamics) of the ring-polymer dividing surface, nor of any surface orthogonal to it in the space describing fluctuations in the polymer-bead positions along the reaction coordinate. In practice, this means that RPMD-TST gives a good approximation to the exact quantum rate for direct reactions, provided the temperature is not too far below the cross-over to deep tunnelling. We derive these results by comparing the t → ∞ limit of the ring-polymer flux-side time-correlation function with that of a hybrid flux-side time-correlation function (containing a ring-polymer flux operator and a Miller-Schwarz-Tromp side function), and by representing the resulting ring-polymer momentum integrals as hypercubes. Together with Paper I, the results of this article validate a large number of RPMD calculations of reaction rates.

  17. Variable SO2 emission rates for Anatahan volcano, the Commonwealth of the Northern Mariana Islands: Implications for deriving arc-wide volatile fluxes from erupting volcanoes

    NASA Astrophysics Data System (ADS)

    Hilton, D. R.; Fischer, T. P.; McGonigle, A. J. S.; de Moor, J. M.

    2007-07-01

    We report new spectroscopic-derived SO2 emission rates for Anatahan volcano, Mariana Islands. Measurements of SO2 fluxes reveal large fluctuations over the 2003-2005 period - from 78 kg s-1 which occurred on the same day as resurgent volcanic activity (March, 2005) to 50 kg s-1 and 25 kg s-1 made days/weeks after the start of eruptive sequences in 2003 and 2004 respectively. Even the lowest values make Anatahan a major global source of SO2 over the past decade. These SO2 emission rates are used to estimate the CO2 flux from the arc as a whole (=3.6 - 40 × 107 mol km-1 yr-1). Such values are significantly higher than estimates derived using other approaches: they are also high compared to other convergent margins (e.g., Central America) where the input flux of CO2 is substantially greater. Our results caution against including volatile fluxes from actively-degassing volcanoes to produce volatile outputs considered representative of entire arc fronts.

  18. Magnitude and variability of Holocene sediment accumulation in Santa Monica Bay, California

    USGS Publications Warehouse

    Sommerfield, C.K.; Lee, H.J.

    2003-01-01

    The spatial variability of Holocene (past 10,000 years) sediment accumulation in Santa Monica Bay (California) was examined to identify controls sediment trapping in a bathymetrically complex coastal embayment and to provide geologic context for the post-industrial sedimentary record and associated pollution gradients. Sediment chronologies based on downcore AMS 14C dates were used to quantify long-term (millennia) accumulation rates in an effort to elucidate particle-transport pathways and sinks. Sediment accumulation rates for the full range of bayfloor environments (50-630 m water depths) range from 22 to 102 mg/cm2/year (15-88 mm/100 year), have an overall mean of 51??21 mg/cm2/year (1??, n=11), and are comparable to rates reported for adjacent borderland basins. Maximal accumulation rates on the Malibu shelf and within a reentrant to Redondo canyon are interpreted to reflect (1) proximity to sediment sources and (2) localized oceanographic and topographic conditions conducive to sediment trapping and deposition. The 14C-derived accumulation rates are 2-10 times lower than rates determined through 210Pb geochronology for the same sites in a related study, revealing that Holocene sediment accumulation has been non-steady-state. Santa Monica Bay is an important sink for suspended matter; averaged over the past several millennia a mass of sediment equivalent to 10-80% of the modern annual river supply is sequestered yearly. Net influx of suspended matter derived from the adjacent Palos Verdes shelf is evinced by a concentration gradient of p,p???-DDE in bayfloor sediments, whereas the distribution of anthropogenic silver suggests transport from Santa Monica shelf to the southeastern boundary of the bay. The results of this study provide new insight to the long-term fates of particulate matter in Los Angeles coastal waters. ?? 2003 Elsevier Science Ltd. All rights reserved.

  19. 19 CFR 10.534 - Accumulation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. United States-Singapore Free Trade Agreement Rules of Origin § 10.534 Accumulation. (a) Originating materials of Singapore or the United States...

  20. 19 CFR 10.534 - Accumulation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. United States-Singapore Free Trade Agreement Rules of Origin § 10.534 Accumulation. (a) Originating materials of Singapore or the United States...

  1. 19 CFR 10.534 - Accumulation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. United States-Singapore Free Trade Agreement Rules of Origin § 10.534 Accumulation. (a) Originating materials of Singapore or the United States...

  2. 19 CFR 10.534 - Accumulation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. United States-Singapore Free Trade Agreement Rules of Origin § 10.534 Accumulation. (a) Originating materials of Singapore or the United States...

  3. 19 CFR 10.534 - Accumulation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. United States-Singapore Free Trade Agreement Rules of Origin § 10.534 Accumulation. (a) Originating materials of Singapore or the United States...

  4. Organic-carbon-rich rocks: Fast or slow organic-carbon accumulation?

    SciTech Connect

    Isaacs, C.M.; Piper, D.Z.; Keller, M.A.

    1996-12-31

    Organic-carbon-rich rocks and sediments are generally attributed in the marine geologic literature to high rates of organic carbon accumulation, resulting either from rapid input and/or excellent preservation. An alternate interpretation suggested by evidence from both oil-source rocks and modern sediments is that many organic-carbon-rich strata result from comparatively slow accumulation of organic carbon that is little diluted. The idea that organic-carbon-rich rocks represent rapid organic-carbon accumulation derives partly from the enhanced organic-carbon preservation associated with faster burial. Re-evaluation of published sediment trap and accumulation rate data in modern oceans shows, however, that sedimentation rate has been highly over-rated as a cause of high organic carbon abundance. As sedimentation rate increases, increased dilution outpaces increased preservation such that, other things being equal, more abundant organic carbon is associated with slower (not faster) sedimentation rates. Compared to an equal thickness of rapidly accumulated organic-carbon-lean sediment in the geologic record, slowly accumulated organic-carbon-rich sediment can represent 10-20 times more time-but be misinterpreted as reflecting rapid organic carbon accumulation by the common practice of interpolating age linearly with strata thickness. This relation explains the {open_quotes}enigma{close_quotes} of transgressive black shales, including numerous oil source-rocks worldwide associated with early phases of sea level rise. In offshore locations (20-200 km from the coast), rising sea level may sharply reduce terrigenous supply without significantly affecting productivity. The result is an organic-carbon-rich condensed zone reflecting neither high productivity nor low bottom-water oxygen nor rapid sedimentation, but simply lack of dilution.

  5. Organic-carbon-rich rocks: Fast or slow organic-carbon accumulation

    SciTech Connect

    Isaacs, C.M.; Piper, D.Z.; Keller, M.A. )

    1996-01-01

    Organic-carbon-rich rocks and sediments are generally attributed in the marine geologic literature to high rates of organic carbon accumulation, resulting either from rapid input and/or excellent preservation. An alternate interpretation suggested by evidence from both oil-source rocks and modern sediments is that many organic-carbon-rich strata result from comparatively slow accumulation of organic carbon that is little diluted. The idea that organic-carbon-rich rocks represent rapid organic-carbon accumulation derives partly from the enhanced organic-carbon preservation associated with faster burial. Re-evaluation of published sediment trap and accumulation rate data in modern oceans shows, however, that sedimentation rate has been highly over-rated as a cause of high organic carbon abundance. As sedimentation rate increases, increased dilution outpaces increased preservation such that, other things being equal, more abundant organic carbon is associated with slower (not faster) sedimentation rates. Compared to an equal thickness of rapidly accumulated organic-carbon-lean sediment in the geologic record, slowly accumulated organic-carbon-rich sediment can represent 10-20 times more time-but be misinterpreted as reflecting rapid organic carbon accumulation by the common practice of interpolating age linearly with strata thickness. This relation explains the [open quotes]enigma[close quotes] of transgressive black shales, including numerous oil source-rocks worldwide associated with early phases of sea level rise. In offshore locations (20-200 km from the coast), rising sea level may sharply reduce terrigenous supply without significantly affecting productivity. The result is an organic-carbon-rich condensed zone reflecting neither high productivity nor low bottom-water oxygen nor rapid sedimentation, but simply lack of dilution.

  6. The Influence of Tissue Ischemia Time on RNA Integrity and Patient-Derived Xenografts (PDX) Engraftment Rate in a Non-Small Cell Lung Cancer (NSCLC) Biobank

    PubMed Central

    Maletta, Francesca; Gaudiano, Marcello; Ercole, Elisabetta; Annaratone, Laura; Todaro, Maria; Boita, Monica; Filosso, Pier Luigi; Solidoro, Paolo; Delsedime, Luisa; Oliaro, Alberto; Sapino, Anna; Ruffini, Enrico; Inghirami, Giorgio

    2016-01-01

    Introduction Bio-repositories are invaluable resources to implement translational cancer research and clinical programs. They represent one of the most powerful tools for biomolecular studies of clinically annotated cohorts, but high quality samples are required to generate reliable molecular readouts and functional studies. The objective of our study was to define the impact of cancer tissue ischemia time on RNA and DNA quality, and for the generation of Patient-Derived Xenografts (PDXs). Methods One-hundred thirty-five lung cancer specimens were selected among our Institutional BioBank samples. Associations between different warm (surgical) and cold (ex-vivo) ischemia time ranges and RNA quality or PDXs engraftment rates were assessed. RNA quality was determined by RNA integrity number (RINs) values. Fresh viable tissue fragments were implanted subcutaneously in NSG mice and serially transplanted. Results RNAs with a RIN>7 were detected in 51% of the sample (70/135), with values of RIN significantly lower (OR 0.08, P = 0.01) in samples preserved for more than 3 hours before cryopreservation. Higher quality DNA samples had a concomitant high RIN. Sixty-three primary tumors (41 adenocarcinoma) were implanted with an overall engraftment rate of 33%. Both prolonged warm (>2 hours) and ex-vivo ischemia time (>10 hours) were associated to a lower engraftment rate (OR 0.09 P = 0.01 and OR 0.04 P = 0.008, respectively). Conclusion RNA quality and PDXs engraftment rate were adversely affected by prolonged ischemia times. Proper tissue collection and processing reduce failure rate. Overall, NSCLC BioBanking represents an innovative modality, which can be successfully executed in routine clinical settings, when stringent Standard Operating Procedures are adopted. PMID:26731692

  7. Derivation of turbulent energy dissipation rate with the Middle Atmosphere Alomar Radar System (MAARSY) and radiosondes at Andøya, Norway

    NASA Astrophysics Data System (ADS)

    Li, Qiang; Rapp, Markus; Schrön, Anne; Schneider, Andreas; Stober, Gunter

    2016-12-01

    We present the derivation of turbulent energy dissipation rate ɛ from a total of 522 days of observations with the Middle Atmosphere Alomar Radar SYstem (MAARSY) mesosphere-stratosphere-troposphere (MST) radar running tropospheric experiments during the period of 2010-2013 as well as with balloon-borne radiosondes based on a campaign in the summer 2013. Spectral widths are converted to ɛ after the removal of the broadening effects due to the finite beam width of the radar. With the simultaneous in situ measurements of ɛ with balloon-borne radiosondes at the MAARSY radar site, we compare the ɛ values derived from both techniques and reach an encouraging agreement between them. Using all the radar data available, we present a preliminary climatology of atmospheric turbulence in the UTLS (upper troposphere and lower stratosphere) region above the MAARSY site showing a variability of more than 5 orders of magnitude inherent in turbulent energy dissipation rates. The derived ɛ values reveal a log-normal distribution with a negative skewness, and the ɛ profiles show an increase with height which is also the case for each individual month. Atmospheric turbulence based on our radar measurements reveals a seasonal variation but no clear diurnal variation in the UTLS region. Comparison of ɛ with the gradient Richardson number Ri shows that only 1.7 % of all the data with turbulence occur under the condition of Ri < 1 and that the values of ɛ under the condition of Ri < 1 are significantly larger than those under Ri > 1. Further, there is a roughly negative correlation between ɛ and Ri that is independent of the scale dependence of Ri. Turbulence under active dynamical conditions (velocity of horizontal wind U > 10 m s-1) is significantly stronger than under quiet conditions (U < 10 m s-1). Last but not least, the derived ɛ values are compared with the corresponding vertical shears of background wind velocity showing a linear relation with a corresponding

  8. Respiratory rate and pulse oximetry derived information as predictors of hospital admission in young children in Bangladesh: a prospective observational study

    PubMed Central

    Garde, Ainara; Zhou, Guohai; Raihana, Shahreen; Dunsmuir, Dustin; Karlen, Walter; Dekhordi, Parastoo; Huda, Tanvir; Arifeen, Shams El; Larson, Charles; Kissoon, Niranjan; Dumont, Guy A; Ansermino, J Mark

    2016-01-01

    Objective Hypoxaemia is a strong predictor of mortality in children. Early detection of deteriorating condition is vital to timely intervention. We hypothesise that measures of pulse oximetry dynamics may identify children requiring hospitalisation. Our aim was to develop a predictive tool using only objective data derived from pulse oximetry and observed respiratory rate to identify children at increased risk of hospital admission. Setting Tertiary-level hospital emergency department in Bangladesh. Participants Children under 5 years (n=3374) presenting at the facility (October 2012–April 2013) without documented chronic diseases were recruited. 1-minute segments of pulse oximetry (photoplethysmogram (PPG), blood oxygen saturation (SpO2) and heart rate (HR)) and respiratory rate were collected with a mobile app. Primary outcome The need for hospitalisation based on expert physician review and follow-up. Methods Pulse rate variability (PRV) using pulse peak intervals of the PPG signal and features extracted from the SpO2 signal, all derived from pulse oximetry recordings, were studied. A univariate age-adjusted logistic regression was applied to evaluate differences between admitted and non-admitted children. A multivariate logistic regression model was developed using a stepwise selection of predictors and was internally validated using bootstrapping. Results Children admitted to hospital showed significantly (p<0.01) decreased PRV and higher SpO2 variability compared to non-admitted children. The strongest predictors of hospitalisation were reduced PRV-power in the low frequency band (OR associated with a 0.01 unit increase, 0.93; 95% CI 0.89 to 0.98), greater time spent below an SpO2 of 98% and 94% (OR associated with 10 s increase, 1.4; 95% CI 1.3 to 1.4 and 1.5; 95% CI 1.4 to 1.6, respectively), high respiratory rate, high HR, low SpO2, young age and male sex. These variables provided a bootstrap-corrected AUC of the receiver operating characteristic

  9. In situ study through electrical resistance of growth rate of trifluoroacetate-based solution-derived YBa2Cu3O7 films

    NASA Astrophysics Data System (ADS)

    Sánchez-Valdés, C. F.; Puig, T.; Obradors, X.

    2015-02-01

    In this work, we have studied by means of in situ electrical measurements the nucleation, growth and sintering stages of epitaxial YBa2Cu3O6+δ (YBCO) superconducting thin films prepared using a chemical solution deposition approach based on metal-organic trifluoroacetate-based (TFA) precursors. Single crystal substrates (LaAlO3 and CeO2/YSZ) were used in this study. Analysis of isothermal time dependences, at different temperatures, of in situ electrical resistance of films allowed to evidence that the growth rate G is strongly temperature dependent, i.e. G is enhanced by a factor ˜15 when going from 700 to 810 °C. Additionally, we demonstrate that adding Ag-TFA in the solution may enhance the growth rate by as much as 50%, as compared to pure YBCO, thus confirming previous assessments of the strong influence of Ag doping on YBCO film growth and microstructure. In situ electrical resistance measurements show as well that an incubation time exists and we infer the origin of its temperature dependence. Finally, a thermodynamic analysis allows proposing a single equation for the growth rate of YBCO films integrating all the relevant processing parameters. Our analysis has validated the solid-gas reaction-diffusion model describing the growth of YBCO films from TFA precursors and thus enlarges the knowledge required to enhance the control of the microstructure and superconducting properties of solution-derived YBCO films.

  10. A comparison of methods for deriving solute flux rates using long-term data from streams in the mirror lake watershed

    USGS Publications Warehouse

    Bukaveckas, P.A.; Likens, G.E.; Winter, T.C.; Buso, D.C.

    1998-01-01

    Calculation of chemical flux rates for streams requires integration of continuous measurements of discharge with discrete measurements of solute concentrations. We compared two commonly used methods for interpolating chemistry data (time-averaging and flow-weighting) to determine whether discrepancies between the two methods were large relative to other sources of error in estimating flux rates. Flux rates of dissolved Si and SO42- were calculated from 10 years of data (1981-1990) for the NW inlet and Outlet of Mirror Lake and for a 40-day period (March 22 to April 30, 1993) during which we augmented our routine (weekly) chemical monitoring with collection of daily samples. The time-averaging method yielded higher estimates of solute flux during high-flow periods if no chemistry samples were collected corresponding to peak discharge. Concentration-discharge relationships should be used to interpolate stream chemistry during changing flow conditions if chemical changes are large. Caution should be used in choosing the appropriate time-scale over which data are pooled to derive the concentration-discharge regressions because the model parameters (slope and intercept) were found to be sensitive to seasonal and inter-annual variation. Both methods approximated solute flux to within 2-10% for a range of solutes that were monitored during the intensive sampling period. Our results suggest that errors arising from interpolation of stream chemistry data are small compared with other sources of error in developing watershed mass balances.

  11. High Genetic and Epigenetic Stability in Coffea arabica Plants Derived from Embryogenic Suspensions and Secondary Embryogenesis as Revealed by AFLP, MSAP and the Phenotypic Variation Rate

    PubMed Central

    Bobadilla Landey, Roberto; Cenci, Alberto; Georget, Frédéric; Bertrand, Benoît; Camayo, Gloria; Dechamp, Eveline; Herrera, Juan Carlos; Santoni, Sylvain; Lashermes, Philippe; Simpson, June; Etienne, Hervé

    2013-01-01

    Embryogenic suspensions that involve extensive cell division are risky in respect to genome and epigenome instability. Elevated frequencies of somaclonal variation in embryogenic suspension-derived plants were reported in many species, including coffee. This problem could be overcome by using culture conditions that allow moderate cell proliferation. In view of true-to-type large-scale propagation of C. arabica hybrids, suspension protocols based on low 2,4-D concentrations and short proliferation periods were developed. As mechanisms leading to somaclonal variation are often complex, the phenotypic, genetic and epigenetic changes were jointly assessed so as to accurately evaluate the conformity of suspension-derived plants. The effects of embryogenic suspensions and secondary embryogenesis, used as proliferation systems, on the genetic conformity of somatic embryogenesis-derived plants (emblings) were assessed in two hybrids. When applied over a 6 month period, both systems ensured very low somaclonal variation rates, as observed through massive phenotypic observations in field plots (0.74% from 200 000 plant). Molecular AFLP and MSAP analyses performed on 145 three year-old emblings showed that polymorphism between mother plants and emblings was extremely low, i.e. ranges of 0–0.003% and 0.07–0.18% respectively, with no significant difference between the proliferation systems for the two hybrids. No embling was found to cumulate more than three methylation polymorphisms. No relation was established between the variant phenotype (27 variants studied) and a particular MSAP pattern. Chromosome counting showed that 7 of the 11 variant emblings analyzed were characterized by the loss of 1–3 chromosomes. This work showed that both embryogenic suspensions and secondary embryogenesis are reliable for true-to-type propagation of elite material. Molecular analyses revealed that genetic and epigenetic alterations are particularly limited during coffee somatic

  12. An Enhancement of 160um-derived Star-Formation Rates in Active Galaxies to z = 3.2 with the ZFOURGE Survey

    NASA Astrophysics Data System (ADS)

    Cowley, Michael; Spitler, L.

    2016-08-01

    ZFOURGE is a new imaging survey, which employs unique near-infrared medium- band filters designed to probe galaxy properties over the last 12 billion years. By supplementing the high-quality ZFOURGE galaxy catalogues with data in radio, X- ray, and infrared wavebands, we have studied the complex interplay between AGNs and their host galaxies to a time when the Universe was only 10-15% of its present age. I will report on our study to compare the 160-um derived star-formation rates in active and non-active galaxies and show that AGN hosts, over all redshifts, tend to exhibit an elevated average star-formation activity compared to their non-active counterparts.

  13. Differential erosion by different-sized glaciers as reflected in 10Be-derived erosion rates of glacier valley walls, Kichatna Mts., Alaska

    NASA Astrophysics Data System (ADS)

    Ward, D.; Anderson, R. S.

    2009-12-01

    The Kichatna Mountains, Alaska Range, Alaska comprise a dramatic landscape carved into a small ~65 Ma granitic pluton about 100 km west of Denali, in which kilometer-tall rock walls and “cathedral” spires tower over a radial array of over a dozen individual valley glaciers. The sheer scale of the relief speaks to the relative rates of valley incision by glaciers and rockwall retreat, but absolute rates are difficult to determine. We use cosmogenic 10Be to measure rockwall backwearing rates (and discuss several very important caveats to this use) on timescales of 103-104 yr, with a straightforward sampling strategy that exploits ablation-dominated medial moraines. In simple cases, a medial moraine and its associated englacial debris serve as a conveyor belt that brings supraglacial rockfall debris from the accumulation zone valley wall to a moraine crest in the ablation zone. Our samples come from the largest medial moraine on