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Sample records for achieve diffraction-limited resolution

  1. Crystallography: Resolution beyond the diffraction limit

    NASA Astrophysics Data System (ADS)

    Shen, Jian-Ren

    2016-02-01

    A method has been devised that extends the resolution of X-ray crystal structures beyond the diffraction limit. This might help to improve the visualization of structures of proteins that form 'poorly diffracting' crystals. See Letter p.202

  2. Multi foci with diffraction limited resolution.

    PubMed

    Waller, Erik H; von Freymann, Georg

    2013-09-09

    The generation of multi foci is an established method for high-speed parallel direct laser writing, scanning microscopy and for optical tweezer arrays. However, the quality of multi foci reduces with increasing resolution due to interference effects. Here, we report on a spatial-light-modulator-based method that allows for highly uniform, close to Gaussian spots with diffraction limited resolution using a wavelength of 780 nm. We introduce modifications of a standard algorithm that calculates a field distribution on the entrance pupil of a high numerical aperture objective splitting the focal volume into a multitude of spots. Our modified algorithm compares favourably to a commonly used algorithm in full vectorial calculations as well as in point-spread-function measurements. The lateral and axial resolution limits of spots generated by the new algorithm are found to be close to the diffraction limit.

  3. Semiconductor-based superlens for subwavelength resolution below the diffraction limit at extreme ultraviolet frequencies

    NASA Astrophysics Data System (ADS)

    Vincenti, M. A.; D'Orazio, A.; Cappeddu, M. G.; Akozbek, Neset; Bloemer, M. J.; Scalora, M.

    2009-05-01

    We theoretically demonstrate negative refraction and subwavelength resolution below the diffraction limit in the UV and extreme UV ranges using semiconductors. The metal-like response of typical semiconductors such as GaAs or GaP makes it possible to achieve negative refraction and superguiding in resonant semiconductor/dielectric multilayer stacks, similar to what has been demonstrated in metallodielectric photonic band gap structures. The exploitation of this basic property in semiconductors raises the possibility of yet-untapped applications in the UV and soft x-ray ranges.

  4. Super-accuracy and super-resolution getting around the diffraction limit.

    PubMed

    Toprak, Erdal; Kural, Comert; Selvin, Paul R

    2010-01-01

    In many research areas such as biology, biochemistry, and biophysics, measuring distances or identifying and counting objects can be of great importance. To do this, researchers often need complicated and expensive tools in order to have accurate measurements. In addition, these measurements are often done under nonphysiological settings. X-ray diffraction, for example, gets Angstrom-level structures, but it requires crystallizing a biological specimen. Electron microscopy (EM) has about 10A resolution, but often requires frozen (liquid nitrogen) samples. Optical microscopy, while coming closest to physiologically relevant conditions, has been limited by the minimum distances to be measured, typically about the diffraction limit, or approximately 200 nm. However, most biological molecules are <5-10nm in diameter, and getting molecular details requires imaging at this scale. In this chapter, we will describe some of the experimental approaches, from our lab and others, that push the limits of localization accuracy and optical resolution in fluorescence microscopy.

  5. Compact high-resolution spectrographs for large and extremely large telescopes: using the diffraction limit

    NASA Astrophysics Data System (ADS)

    Robertson, J. Gordon; Bland-Hawthorn, Joss

    2012-09-01

    As telescopes get larger, the size of a seeing-limited spectrograph for a given resolving power becomes larger also, and for ELTs the size will be so great that high resolution instruments of simple design will be infeasible. Solutions include adaptive optics (but not providing full correction for short wavelengths) or image slicers (which give feasible but still large instruments). Here we develop the solution proposed by Bland-Hawthorn and Horton: the use of diffraction-limited spectrographs which are compact even for high resolving power. Their use is made possible by the photonic lantern, which splits a multi-mode optical fiber into a number of single-mode fibers. We describe preliminary designs for such spectrographs, at a resolving power of R ~ 50,000. While they are small and use relatively simple optics, the challenges are to accommodate the longest possible fiber slit (hence maximum number of single-mode fibers in one spectrograph) and to accept the beam from each fiber at a focal ratio considerably faster than for most spectrograph collimators, while maintaining diffraction-limited imaging quality. It is possible to obtain excellent performance despite these challenges. We also briefly consider the number of such spectrographs required, which can be reduced by full or partial adaptive optics correction, and/or moving towards longer wavelengths.

  6. Enhancement of image resolution beyond the diffraction limit by double dark resonances

    NASA Astrophysics Data System (ADS)

    Verma, Onkar N.; Dey, Tarak N.

    2014-03-01

    We show how quantum coherence effects can be used to improve the resolution and the contrast of diffraction-limited images imprinted onto a probe field. The narrow and sharp spectral features generated by double dark resonances (DDR) are exploited to control absorption, dispersion, and diffraction properties of the medium. The spatially modulated control field can produce inhomogeneous susceptibility of the medium that encodes the spatial feature of the control image to probe field in the presence of DDR. The transmission of a cloned image can be enhanced by the use of an incoherent pump field. We find that the feature size of the cloned image is four times smaller than the initial characteristic size of the control image even though the control image is completely distorted after propagation through a 3-cm-long Rb vapor cell. We further discuss how spatial optical switching is possible by using induced transparency and absorption of the medium.

  7. Metalenses at visible wavelengths: Diffraction-limited focusing and subwavelength resolution imaging.

    PubMed

    Khorasaninejad, Mohammadreza; Chen, Wei Ting; Devlin, Robert C; Oh, Jaewon; Zhu, Alexander Y; Capasso, Federico

    2016-06-03

    Subwavelength resolution imaging requires high numerical aperture (NA) lenses, which are bulky and expensive. Metasurfaces allow the miniaturization of conventional refractive optics into planar structures. We show that high-aspect-ratio titanium dioxide metasurfaces can be fabricated and designed as metalenses with NA = 0.8. Diffraction-limited focusing is demonstrated at wavelengths of 405, 532, and 660 nm with corresponding efficiencies of 86, 73, and 66%. The metalenses can resolve nanoscale features separated by subwavelength distances and provide magnification as high as 170×, with image qualities comparable to a state-of-the-art commercial objective. Our results firmly establish that metalenses can have widespread applications in laser-based microscopy, imaging, and spectroscopy.

  8. Mode-converters for rectangular-core fiber amplifiers to achieve diffraction-limited power scaling.

    PubMed

    Sridharan, Arun Kumar; Pax, Paul H; Heebner, John E; Drachenberg, Derrek R; Armstrong, J Paul; Dawson, Jay W

    2012-12-17

    A rectangular-core (ribbon) fiber that guides and amplifies a single higher-order-mode (HOM) can potentially scale to much higher average powers than what is possible in traditional circular-core large-mode-area fibers. Such an amplifier would require mode-conversion at the input to enable interfacing with seed sources that typically output TEM(00) mode radiation and at the output to generate diffraction-limited radiation for end-user applications. We present the first simulation and experimental results of a mode conversion technique that uses two diffractive-optic-elements in conjugate Fourier planes to convert a diffraction limited TEM(00) mode to the HOM of a ribbon fiber. Mode-conversion-efficiency is approximately 84% and can theoretically approach 100%. We also demonstrate a mode-converter system that converts a single HOM of a ribbon fiber back to a diffraction-limited TEM(00) mode. Conversion efficiency is a record 80.5%.

  9. Diffraction-limited spatial resolution of circumstellar shells at 10 microns

    NASA Technical Reports Server (NTRS)

    Bloemhof, E. E.; Townes, C. H.; Vanderwyck, A. H. B.

    1983-01-01

    A new spatial array instrument provided diffraction-limited mid-infrared intensity profiles of the type-M supergiant stars alpha Orionis and alpha Scorpii, both of which are known to exhibit excess 10 microns radiation due to the presence of circumstellar dust shells. In the case of alpha Ori, there is a marked asymmetry in the dust distribution, with peak intensity of dust emission a distance of 0.9 inches from the star.

  10. Breaking the diffraction-limited resolution barrier in fiber-optical two-photon fluorescence endoscopy by an azimuthally-polarized beam.

    PubMed

    Gu, Min; Kang, Hong; Li, Xiangping

    2014-01-10

    Although fiber-optical two-photon endoscopy has been recognized as a potential high-resolution diagnostic and therapeutic procedure in vivo, its resolution is limited by the optical diffraction nature to a few micrometers due to the low numerical aperture of an endoscopic objective. On the other hand, stimulated emission depletion (STED) achieved by a circularly-polarized vortex beam has been used to break the diffraction-limited resolution barrier in a bulky microscope. It has been a challenge to apply the STED principle to a fiber-optical two-photon endoscope as a circular polarization state cannot be maintained due to the birefringence of a fiber. Here, we demonstrate the first fiber-optical STED two-photon endoscope using an azimuthally-polarized beam directly generated from a double-clad fiber. As such, the diffraction-limited resolution barrier of fiber-optical two-photon endoscopy can be broken by a factor of three. Our new accomplishment has paved a robust way for high-resolution in vivo biomedical studies.

  11. Breaking the diffraction-limited resolution barrier in fiber-optical two-photon fluorescence endoscopy by an azimuthally-polarized beam

    NASA Astrophysics Data System (ADS)

    Gu, Min; Kang, Hong; Li, Xiangping

    2014-01-01

    Although fiber-optical two-photon endoscopy has been recognized as a potential high-resolution diagnostic and therapeutic procedure in vivo, its resolution is limited by the optical diffraction nature to a few micrometers due to the low numerical aperture of an endoscopic objective. On the other hand, stimulated emission depletion (STED) achieved by a circularly-polarized vortex beam has been used to break the diffraction-limited resolution barrier in a bulky microscope. It has been a challenge to apply the STED principle to a fiber-optical two-photon endoscope as a circular polarization state cannot be maintained due to the birefringence of a fiber. Here, we demonstrate the first fiber-optical STED two-photon endoscope using an azimuthally-polarized beam directly generated from a double-clad fiber. As such, the diffraction-limited resolution barrier of fiber-optical two-photon endoscopy can be broken by a factor of three. Our new accomplishment has paved a robust way for high-resolution in vivo biomedical studies.

  12. Rapid super-resolution imaging of sub-surface nanostructures beyond diffraction limit by high refractive index microsphere optical nanoscopy

    NASA Astrophysics Data System (ADS)

    Lee, Seoungjun; Li, Lin

    2015-01-01

    Sub-surface nanostructures cannot be observed by scanning electronic microscopy or standard scanning probe microscopy. They are also outside the resolution limit of standard optical microscopes. In this paper, we demonstrate super-resolution imaging of sub-surface nanostructures beyond the optical diffraction limit. Sub-surface Blu-ray recorded data structures (100-200 nm) have been observed directly with submerged microsphere optical nanoscopy (SMON) using TiO2-BaO-ZnO glass microspheres (refractive index=2.2) of 60 μm diameter immersed in water coupled with a standard optical microscope. Theoretical analysis of the imaging phenomena was carried out by the characteristics of electrical field Poynting vectors and photonic nanojets.

  13. Compact terahertz time domain spectroscopy system with diffraction-limited spatial resolution

    NASA Astrophysics Data System (ADS)

    Watanabe, Shinichi; Shimano, Ryo

    2007-10-01

    A compact and rigid terahertz time domain spectroscopy system is presented. The size of the device is 20mm diameter times 61mm length with four parabolic-shaped concave mirrors dug in it to effectively focus a terahertz beam on a sample. The device has no chromatic aberration over the whole bandwidth of the beam (0.3-2THz), and an effective numerical aperture of about 0.45 is achieved, which has a capability to image the structure whose size is almost the same as the wavelength. Frequency resolved images clearly show this performance. We also show that quantitative retrieval of the complex refractive index of the structure as small as twice of the wavelength is possible.

  14. Prospects of high-resolution resonant X-ray inelastic scattering studies on solid materials, liquids and gases at diffraction-limited storage rings

    PubMed Central

    Schmitt, Thorsten; de Groot, Frank M. F.; Rubensson, Jan-Erik

    2014-01-01

    The spectroscopic technique of resonant inelastic X-ray scattering (RIXS) will particularly profit from immensely improved brilliance of diffraction-limited storage rings (DLSRs). In RIXS one measures the intensities of excitations as a function of energy and momentum transfer. DLSRs will allow for pushing the achievable energy resolution, signal intensity and the sampled spot size to new limits. With RIXS one nowadays probes a broad range of electronic systems reaching from simple molecules to complex materials displaying phenomena like peculiar magnetism, two-dimensional electron gases, superconductivity, photovoltaic energy conversion and heterogeneous catalysis. In this article the types of improved RIXS studies that will become possible with X-ray beams from DLSRs are envisioned. PMID:25177995

  15. Diffraction-limited ultrabroadband terahertz spectroscopy

    NASA Astrophysics Data System (ADS)

    Baillergeau, M.; Maussang, K.; Nirrengarten, T.; Palomo, J.; Li, L. H.; Linfield, E. H.; Davies, A. G.; Dhillon, S.; Tignon, J.; Mangeney, J.

    2016-05-01

    Diffraction is the ultimate limit at which details of objects can be resolved in conventional optical spectroscopy and imaging systems. In the THz spectral range, spectroscopy systems increasingly rely on ultra-broadband radiation (extending over more 5 octaves) making a great challenge to reach resolution limited by diffraction. Here, we propose an original easy-to-implement wavefront manipulation concept to achieve ultrabroadband THz spectroscopy system with diffraction-limited resolution. Applying this concept to a large-area photoconductive emitter, we demonstrate diffraction-limited ultra-broadband spectroscopy system up to 14.5 THz with a dynamic range of 103. The strong focusing of ultrabroadband THz radiation provided by our approach is essential for investigating single micrometer-scale objects such as graphene flakes or living cells, and besides for achieving intense ultra-broadband THz electric fields.

  16. Diffraction-limited ultrabroadband terahertz spectroscopy

    PubMed Central

    Baillergeau, M.; Maussang, K.; Nirrengarten, T.; Palomo, J.; Li, L. H.; Linfield, E. H.; Davies, A. G.; Dhillon, S.; Tignon, J.; Mangeney, J.

    2016-01-01

    Diffraction is the ultimate limit at which details of objects can be resolved in conventional optical spectroscopy and imaging systems. In the THz spectral range, spectroscopy systems increasingly rely on ultra-broadband radiation (extending over more 5 octaves) making a great challenge to reach resolution limited by diffraction. Here, we propose an original easy-to-implement wavefront manipulation concept to achieve ultrabroadband THz spectroscopy system with diffraction-limited resolution. Applying this concept to a large-area photoconductive emitter, we demonstrate diffraction-limited ultra-broadband spectroscopy system up to 14.5 THz with a dynamic range of 103. The strong focusing of ultrabroadband THz radiation provided by our approach is essential for investigating single micrometer-scale objects such as graphene flakes or living cells, and besides for achieving intense ultra-broadband THz electric fields. PMID:27142959

  17. Printing colour at the optical diffraction limit.

    PubMed

    Kumar, Karthik; Duan, Huigao; Hegde, Ravi S; Koh, Samuel C W; Wei, Jennifer N; Yang, Joel K W

    2012-09-01

    The highest possible resolution for printed colour images is determined by the diffraction limit of visible light. To achieve this limit, individual colour elements (or pixels) with a pitch of 250 nm are required, translating into printed images at a resolution of ∼100,000 dots per inch (d.p.i.). However, methods for dispensing multiple colourants or fabricating structural colour through plasmonic structures have insufficient resolution and limited scalability. Here, we present a non-colourant method that achieves bright-field colour prints with resolutions up to the optical diffraction limit. Colour information is encoded in the dimensional parameters of metal nanostructures, so that tuning their plasmon resonance determines the colours of the individual pixels. Our colour-mapping strategy produces images with both sharp colour changes and fine tonal variations, is amenable to large-volume colour printing via nanoimprint lithography, and could be useful in making microimages for security, steganography, nanoscale optical filters and high-density spectrally encoded optical data storage.

  18. 3-D Ultrasound Localization Microscopy for Identifying Microvascular Morphology Features of Tumor Angiogenesis at a Resolution Beyond the Diffraction Limit of Conventional Ultrasound

    PubMed Central

    Lin, Fanglue; Shelton, Sarah E.; Espíndola, David; Rojas, Juan D.; Pinton, Gianmarco; Dayton, Paul A.

    2017-01-01

    Angiogenesis has been known as a hallmark of solid tumor cancers for decades, yet ultrasound has been limited in its ability to detect the microvascular changes associated with malignancy. Here, we demonstrate the potential of 'ultrasound localization microscopy' applied volumetrically in combination with quantitative analysis of microvascular morphology, as an approach to overcome this limitation. This pilot study demonstrates our ability to image complex microvascular patterns associated with tumor angiogenesis in-vivo at a resolution of tens of microns - substantially better than the diffraction limit of traditional clinical ultrasound, yet using an 8 MHz clinical ultrasound probe. Furthermore, it is observed that data from healthy and tumor-bearing tissue exhibit significant differences in microvascular pattern and density. Results suggests that with continued development of these novel technologies, ultrasound has the potential to detect biomarkers of cancer based on the microvascular 'fingerprint' of malignant angiogenesis rather than through imaging of blood flow dynamics or the tumor mass itself. PMID:28042327

  19. Quantum imaging beyond the diffraction limit by optical centroid measurements.

    PubMed

    Tsang, Mankei

    2009-06-26

    I propose a quantum imaging method that can beat the Rayleigh-Abbe diffraction limit and achieve de Broglie resolution without requiring a multiphoton absorber or coincidence detection. Using the same nonclassical states of light as those for quantum lithography, the proposed method requires only optical intensity measurements, followed by image postprocessing, to produce the same complex quantum interference patterns as those in quantum lithography. The method is expected to be experimentally realizable using current technology.

  20. Formation of optical fields of stimulated Raman scattering with a resolution beyond the Abbe diffraction limit by spherical microlens cavities with whispering gallery modes: Near-field approximation

    NASA Astrophysics Data System (ADS)

    Jouravlev, M. V.

    2012-04-01

    We consider a significant lowering of the threshold of stimulated Raman scattering in solid fused silica spherical microlenses cavities that is caused by an increase in the integral overlap factor of whispering gallery modes. The structure of focal regions of a microlens is shown to have the shape of honeycombs, forming a photonic crystal or a photonic nanojet. We show that, at comparatively small numerical apertures NA = 0.7-0.8, which correspond to hemispherical microlenses, a spherical microlens cavity exhibits the possibility of focusing laser radiation beyond the Abbe diffraction limit. This enables the possibility of wide practical applications of microspheres as a focusing element the resolving power of which exceeds the Abbe diffraction limit in the near field. The whispering-gallery-mode spherical microlens cavity makes it possible to perform laser generation with a duration of a coherent pulse in the subfemtosecond range and to form a subwavelength focal region of the near field. This ensures the possibility of detecting single molecules of a substance in the subwavelength range in the near field and can be used to increase the sensitivity of intracavity spectroscopy methods and as microlasers for excitation of molecules in metal molecular nanoswitches and semiconductor heterostructures. From an array of microlens cavities, metamaterials with a negative refractive index can be formed.

  1. Optical microscopy beyond the diffraction limit

    PubMed Central

    Smolyaninov, Igor I.

    2008-01-01

    Over the past century the resolution of far-field optical microscopes, which rely on propagating optical modes, was widely believed to be limited because of diffraction to a value on the order of a half-wavelength λ∕2 of the light used. Although immersion microscopes had slightly improved resolution on the order of λ∕2n, the increased resolution was limited by the small range of refractive indices, n, of available transparent materials. We are experiencing quick demolition of the diffraction limit in optical microscopy. Over the past few years numerous nonlinear optical microscopy techniques based on photoswitching and saturation of fluorescence demonstrated far-field resolution of 20 to 30 nm. The latest exciting example of these techniques has been demonstrated by Huang et al. [Science 319, 810–813 (2008)]. Moreover, recent progress in metamaterials indicates that artificial optical media can be created, which do not exhibit the diffraction limit. Resolution of linear “immersion” microscopes based on such metamaterials appears limited only by losses, which can be compensated by gain media. Thus, optical microscopy is quickly moving towards the 10 nm resolution scale, which should bring about numerous revolutionary advances in biomedical imaging. PMID:19404465

  2. Physics issues in diffraction limited storage ring design

    NASA Astrophysics Data System (ADS)

    Fan, Wei; Bai, ZhengHe; Gao, WeiWei; Feng, GuangYao; Li, WeiMin; Wang, Lin; He, DuoHui

    2012-05-01

    Diffraction limited electron storage ring is considered a promising candidate for future light sources, whose main characteristics are higher brilliance, better transverse coherence and better stability. The challenge of diffraction limited storage ring design is how to achieve the ultra low beam emittance with acceptable nonlinear performance. Effective linear and nonlinear parameter optimization methods based on Artificial Intelligence were developed for the storage ring physical design. As an example of application, partial physical design of HALS (Hefei Advanced Light Source), which is a diffraction limited VUV and soft X-ray light source, was introduced. Severe emittance growth due to the Intra Beam Scattering effect, which is the main obstacle to achieve ultra low emittance, was estimated quantitatively and possible cures were discussed. It is inspiring that better performance of diffraction limited storage ring can be achieved in principle with careful parameter optimization.

  3. Dynamic microscale temperature gradient in a gold nanorod solution measured by diffraction-limited nanothermometry

    SciTech Connect

    Li, Chengmingyue; Gan, Xiaosong; Li, Xiangping; Gu, Min

    2015-09-21

    We quantify the dynamic microscale temperature gradient in a gold nanorod solution using quantum-dot-based microscopic fluorescence nanothermometry. By incorporating CdSe quantum dots into the solution as a nanothermometer, precise temperature mapping with diffraction-limited spatial resolution and sub-degree temperature resolution is achieved. The acquired data on heat generation and dissipation show an excellent agreement with theoretical simulations. This work reveals an effective approach for noninvasive temperature regulation with localized nanoheaters in microfluidic environment.

  4. Sub-diffraction limit resolution in microscopy

    NASA Technical Reports Server (NTRS)

    Cheng, Ming (Inventor); Chen, Weinong (Inventor)

    2007-01-01

    A method and apparatus for visualizing sub-micron size particles employs a polarizing microscope wherein a focused beam of polarized light is projected onto a target, and a portion of the illuminating light is blocked from reaching the specimen, whereby to produce a shadow region, and projecting diffracted light from the target onto the shadow region.

  5. Quantum interference fringes beating the diffraction limit.

    PubMed

    Kawabe, Yoshio; Fujiwara, Hideki; Okamoto, Ryo; Sasaki, Keiji; Takeuchi, Shigeki

    2007-10-17

    Spatially formed two-photon interference fringes with fringe periods smaller than the diffraction limit are demonstrated. In the experiment, a fringe formed by two-photon NOON states with wavelength lambda=702.2 nm is observed using a specially developed near-field scanning optical microscope probe and two-photon detection setup. The observed fringe period of 328.2 nm is well below the diffraction limit (351 nm = lambda /2). Another experiment with a path-length difference larger than the coherent length of photons confirms that the observed fringe is due to two-photon interference.

  6. Controllable design of super-oscillatory planar lenses for sub-diffraction-limit optical needles.

    PubMed

    Diao, Jinshuai; Yuan, Weizheng; Yu, Yiting; Zhu, Yechuan; Wu, Yan

    2016-02-08

    Sub-diffraction-limit optical needle can be created by a binary amplitude mask through tailoring the interference of diffraction beams. In this paper, a controllable design of super-oscillatory planar lenses to create sub-diffraction-limit optical needles with the tunable focal length and depth of focus (DOF) is presented. As a high-quality optical needle is influenced by various factors, we first propose a multi-objective and multi-constraint optimization model compromising all the main factors to achieve a needle with the prescribed characteristics. The optimizing procedure is self-designed using the Matlab programming language based on the genetic algorithm (GA) and fast Hankel transform algorithm. Numerical simulations show that the optical needles' properties can be controlled accurately. The optimized results are further validated by the theoretical calculation with the Rayleigh-Sommerfeld integral. The sub-diffraction-limit optical needles can be used in wide fields such as optical nanofabrication, super-resolution imaging, particle acceleration and high-density optical data storage.

  7. Achieving High Spatial Resolution Surface Plasmon Resonance Microscopy with Image Reconstruction.

    PubMed

    Yu, Hui; Shan, Xiaonan; Wang, Shaopeng; Tao, Nongjian

    2017-03-07

    Surface plasmon resonance microscopy (SPRM) is a powerful platform for biomedical imaging and molecular binding kinetics analysis. However, the spatial resolution of SPRM along the plasmon propagation direction (longitudinal) is determined by the decaying length of the plasmonic wave, which can be as large as tens of microns. Different methods have been proposed to improve the spatial resolution, but each at the expense of decreased sensitivity or temporal resolution. Here we present a method to achieve high spatial resolution SPRM based on deconvolution of complex field. The method does not require additional optical setup and improves the spatial resolution in the longitudinal direction. We applied the method to image nanoparticles and achieved close-to-diffraction limit resolution in both longitudinal and transverse directions.

  8. Near diffraction limited mid-IR spectromicroscopy using frequency upconversion

    NASA Astrophysics Data System (ADS)

    Sanders, Nicolai; Dam, Jeppe Seidelin; Tidemand-Lichtenberg, Peter; Pedersen, Christian

    2014-02-01

    Mid-infrared microscopy and spectroscopy is interesting due to its medical, biological and chemical applications. Spectromicroscopy can be used for histopathology, sample analysis and diagnosis. The ability to do spectromicroscopy in the 2.5 to 4.5 μm wavelength range where many organic molecules have their fundamental vibrations, with the addition of sufficient spectroscopic resolution to resolve these bands, can e.g. potentially allow for diagnostics without the need for staining of the sample. On a longer timeframe, mid-IR spectromicroscopy has the potential for in-vivo diagnostics, combining morphological and spectral imaging. Recent developments in nonlinear frequency upconversion, have demonstrated the potential to perform both imaging and spectroscopy in the mid-IR range at unparalleled low levels of illumination, the low upconversion detector noise being orders of magnitude below competing technologies. With these applications in mind, we have incorporated microscopy optics into an image upconversion system, achieving near diffraction limited spatial resolution in the 3 μm range. Spectroscopic information is further acquired by appropriate control of the phase match condition of the upconversion process. Multispectral images for a region of interest can be obtained by XY-scanning this region of interest within the field of view of the mid-IR upconversion system. Thus, the whole region of interest can be imaged with all available converter wavelengths, and the spectral representation becomes equal for all points in the image. In addition, the range of converted/imaged wavelengths can be tuned continuously by changing the temperature of the crystal, or discretely by using a different poling channel in the PPLN crystal.

  9. Diffraction-limited step-zoom telescope by image restoration.

    PubMed

    Araiza-Durán, José A; Luna, Esteban; Cornejo-Rodríguez, Alejandro; Sohn, Erika

    2015-11-10

    The design of a step-zoom telescope and its ability to achieve a diffraction-limited performance is explored. The basic idea is to include digital postprocessing to compensate for changes in the modulation transfer function of the system, assuming the knowledge of the range to the object. The instrument is conformed of a two-mirror telescope, two lenses, and a detector. High-quality images and a zoom telescope that ranges from 22 to 61 f-number is achieved by moving the primary mirror and two lenses. The preliminary calculations for the design process and a simulation that shows the performance of the step-zoom telescope are described.

  10. X-ray nanoprobes and diffraction-limited storage rings: opportunities and challenges of fluorescence tomography of biological specimens

    PubMed Central

    de Jonge, Martin D.; Ryan, Christopher G.; Jacobsen, Chris J.

    2014-01-01

    X-ray nanoprobes require coherent illumination to achieve optic-limited resolution, and so will benefit directly from diffraction-limited storage rings. Here, the example of high-resolution X-ray fluorescence tomography is focused on as one of the most voracious demanders of coherent photons, since the detected signal is only a small fraction of the incident flux. Alternative schemes are considered for beam delivery, sample scanning and detectors. One must consider as well the steps before and after the X-ray experiment: sample preparation and examination conditions, and analysis complexity due to minimum dose requirements and self-absorption. By understanding the requirements and opportunities for nanoscale fluorescence tomography, one gains insight into the R&D challenges in optics and instrumentation needed to fully exploit the source advances that diffraction-limited storage rings offer. PMID:25177992

  11. Design Of A Near Diffraction Limited Catadioptric Lens

    NASA Astrophysics Data System (ADS)

    Rao, D. V. B.

    1987-06-01

    A near diffraction limited catadioptric lens of EFL=324.4 mm and f/3.6 was designed for the spectral range 546 to 852 nm. This is a 5 element lens with a field of view of +/-2.5°. The obscuration ratio is 0.5 and relative illumination at the edge of the field is 81.4%. The distortion is less than 0.16%. This lens can be used for high resolution imaging applications using CCDs. The design details were presented in this paper.

  12. A method to overcome the diffraction limit in infrared microscopy using standing waves in an attenuated total reflection configuration

    NASA Astrophysics Data System (ADS)

    Hendaoui, Nordine; Mani, Aladin; Liu, Ning; Tofail, Syed M.; Silien, Christophe; Peremans, André

    2017-01-01

    A method is proposed to overcome the diffraction limit of spatial resolution in infrared microscopy. To achieve this, standing waves in an attenuated total reflection configuration were generated to spatially modulate the absorbance of adsorbate vibrational transitions. A numerical simulation was undertaken. It showed that chemical imaging with a spatial resolution of approximately 100 nm is achievable in the case of self-assembled patterns (ofoctdecyltrichlorosilane [CH3-(CH2)17-SiCl3]), when probing the methyl modes located near 3.5 micrometres.

  13. Sub-diffraction-limited interference photolithography with metamaterials.

    PubMed

    Xu, Ting; Zhao, Yanhui; Ma, Junxian; Wang, Changtao; Cui, Jianhua; Du, Chunlei; Luo, Xiangang

    2008-09-01

    We present that an interference lithography technique beyond the diffraction limit can be theoretically achieved by positing an anisotropic metamaterial under the conventional lithographic mask. Based on the special dispersion characteristics of the metamaterial, only the enhanced evanescent waves with high spatial frequencies can transmit through the metamaterial and contribute to the lithography process. Rigorous coupled wave analysis shows that with 442nm exposure light, one-dimensional periodical structures with 40nm features can be patterned. This technique provides an alternative method to fabricate large-area nanostructures.

  14. Sub-diffraction-limited multilayer coatings for the 0.3 numerical aperture micro-exposure tool for extreme ultraviolet lithography.

    PubMed

    Soufli, Regina; Hudyma, Russell M; Spiller, Eberhard; Gullikson, Eric M; Schmidt, Mark A; Robinson, Jeff C; Baker, Sherry L; Walton, Christopher C; Taylor, John S

    2007-06-20

    Multilayer coating results are discussed for the primary and secondary mirrors of the micro-exposure tool (MET): a 0.30 NA lithographic imaging system with a 200 microm x 600 microm field of view at the wafer plane, operating in the extreme ultraviolet (EUV) region at an illumination wavelength around 13.4 nm. Mo/Si multilayers were deposited by DC-magnetron sputtering on large-area, curved MET camera substrates. A velocity modulation technique was implemented to consistently achieve multilayer thickness profiles with added figure errors below 0.1 nm rms demonstrating sub-diffraction-limited performance, as defined by the classical diffraction limit of Rayleigh (0.25 waves peak to valley) or Marechal (0.07 waves rms). This work is an experimental demonstration of sub-diffraction- limited multilayer coatings for high-NA EUV imaging systems, which resulted in the highest resolution microfield EUV images to date.

  15. Shaping the spatial and spectral emissivity at the diffraction limit

    SciTech Connect

    Makhsiyan, Mathilde; Bouchon, Patrick Jaeck, Julien; Pelouard, Jean-Luc; Haïdar, Riad

    2015-12-21

    Metasurfaces have attracted a growing interest for their ability to artificially tailor an electromagnetic response on various spectral ranges. In particular, thermal sources with unprecedented abilities, such as directionality or monochromaticity, have been achieved. However, these metasurfaces exhibit homogeneous optical properties whereas the spatial modulation of the emissivity up to the wavelength scale is at the crux of the design of original emitters. In this letter, we study an inhomogeneous metasurface made of a nonperiodic set of optical nano-antennas that spatially and spectrally control the emitted light up to the diffraction limit. Each antenna acts as an independent deep subwavelength emitter for given polarization and wavelength. Their juxtaposition at the subwavelength scale encodes far field multispectral and polarized images. This opens up promising breakthroughs for applications such as optical storage, anti-counterfeit devices, and multispectral emitters for biochemical sensing.

  16. Diffraction-limited performance of grazing incidence optical systems

    NASA Technical Reports Server (NTRS)

    Harvey, James E.

    1986-01-01

    Diffraction effects of X-ray optical systems are often (justifiably) ignored due to the small wavelength of the X-ray radiation. However, the extremely large obscuration ratio inherent to grazing incidence optical systems produces a profound degradation of the diffraction image over that produced by a moderately obscured aperture of the same diameter. The contradictory requirements of large collecting area and relatively short length of optical elements has tended to result in proposed designs containing many concentric shells with increasingly higher obscuration ratios. In this paper it is shown that diffraction effects in such systems can significantly affect the achievable optical performance at the low energy (long wavelength) end of the intended operating spectral range. Parametric diffraction-limited performance predictions for both imaging and spectrographic applications will be presented and compared to AXAF performance goals and/or BBXRT fabrication techniques.

  17. Development of next-generation nanolithography methods to break the optical diffraction limit

    NASA Astrophysics Data System (ADS)

    Luo, Huiwen; Wang, Liang; Qin, Jin; Ding, Li

    2016-10-01

    Photolithography has been one of the most important technologies in modern society, especially in semiconductor industry. However, due to the limitation of optical diffraction, this technique becomes more and more complex and expensive. In this paper, we experimentally study two promising techniques, near-field scanning optical lithography and nanoimprint lithography, which both have been proved to be alternatives to photolithography, and achieve sub-wavelength resolution. Taking advantage of bowtie apertures, near-field scanning optical lithography can achieve high resolution beyond the Rayleigh diffractive limit. Here, we report a novel method to fabricate bowtie aperture with sub-15 nm gap, producing highly confined electric near-field by localized surface plasmon (LSP) excitation and nanofocusing of the closely tapered gap, and obtain lithography results with 21 nm resolution (FWHM).We also develop a new plate-to-roll nanoimprint lithography (P2RNIL). Compared with plate-to-plate nanoimprint lithography (P2PNIL) and roll-to-plate nanoimprint lithography (R2PNIL), it avoids cylinder template fabrication in P2RNIL and significantly improves the productivity in P2PNIL. Our P2RNIL system can realize large-area nanoimprint continuously with high resolution and high speed.

  18. Coherent imaging at the diffraction limit

    PubMed Central

    Thibault, Pierre; Guizar-Sicairos, Manuel; Menzel, Andreas

    2014-01-01

    X-ray ptychography, a scanning coherent diffractive imaging technique, holds promise for imaging with dose-limited resolution and sensitivity. If the foreseen increase of coherent flux by orders of magnitude can be matched by additional technological and analytical advances, ptychography may approach imaging speeds familiar from full-field methods while retaining its inherently quantitative nature and metrological versatility. Beyond promises of high throughput, spectroscopic applications in three dimensions become feasible, as do measurements of sample dynamics through time-resolved imaging or careful characterization of decoherence effects. PMID:25177990

  19. Photonic nanojet of cylindrical metalens assembled by hexagonally arranged nanofibers for breaking the diffraction limit.

    PubMed

    Yue, Liyang; Yan, Bing; Wang, Zengbo

    2016-04-01

    We designed a novel cylindrical metalens assembled by hexagonally arranged close-contact nanofibers. A near-field focusing nanojet with a full-width at half-maximum (FWHM) waist, 26.7% smaller than the Abbe diffraction limit for 532 nm wavelength light, is observed at the bottom of a 1600 nm diameter cylindrical metalens assembled by 160 nm diameter nanofibers irradiated by a plane wave from the top. Using differently sized nanofibers as building blocks to assemble the metalens, the waist size of the produced photonic nanojet in the near-field zone and the lateral resolution of the focus can be flexibly adjusted, simultaneously breaking the diffraction limit.

  20. Inexpensive Demonstration of Diffraction-Limited Telescope from NASA Stratospheric Balloons

    NASA Astrophysics Data System (ADS)

    Young, Elliot

    NASA s Balloon Program often flies payloads to altitudes of 120,000 ft or higher, above 99.5% of the atmosphere. At those altitudes, the imaging degradation due to atmospheric- induced wavefront errors is virtually zero. In 2009, the SUNRISE balloon mission quantified the wavefront errors with a Shack-Hartmann array and found no evidence of wavefront errors. This means that a large telescope on a balloon should be able to achieve diffraction-limited performance, provided it can be stabilized at a level that is finer than the diffraction limit. At visible wavelengths, the diffraction limit of a 1 or 2 m telescope is 0.1 arcsec or 0.05 arcsec, respectively. NASA recently demonstrated WASP (the Wallops Arc-Second Pointing system) on a balloon flight in October 2011, a coarse pointing system that kept a dummy telescope (24 ft long, 1500 lbs) stabilized at the 0.25 arcsec level. We propose to use an orthogonal transfer CCD (OTCCD) from MIT Lincoln Laboratory to improve the pointing to 0.05 arcsec, an order of magnitude better than the coarse pointing alone and sufficient to provide long integrations at the diffraction limit of a 2-m telescope. Imaging in visible wavelengths is an important new capability. Ground-based adaptive optics (AO) systems on 8-m and 10-m class telescope cannot effectively correct for atmospheric turbulence at wavelengths shorter than 1 μm; the atmospheric wavefront errors are larger at these wavelengths than in the infrared J-H-K bands. At present, only the Hubble Space Telescope can achieve 0.05 arcsec resolution images in visible wavelengths, a capability that is dramatically oversubscribed. With a camera based on an MIT/LL OTCCD, a 2-m balloon-borne telescope could match the spatial resolution of HST. Under this project (and in conjunction with a SWRI Internal Research proposal), we will perform ground tests of a motion-compensation camera based on an MIT/LL Orthogonal Transfer CCD (OTCCD). This device can shift charge in four directions

  1. Reaching the Diffraction Limit - Differential Speckle and Wide-Field Imaging for the WIYN Telescope

    NASA Technical Reports Server (NTRS)

    Scott, Nic J.; Howell, Steve; Horch, Elliott

    2016-01-01

    Speckle imaging allows telescopes to achieve diffraction limited imaging performance. The technique requires cameras capable of reading out frames at a very fast rate, effectively 'freezing out' atmospheric seeing. The resulting speckles can be correlated and images reconstructed that are at the diffraction limit of the telescope. These new instruments are based on the successful performance and design of the Differential Speckle Survey Instrument (DSSI).The instruments are being built for the Gemini-N and WIYN telescopes and will be made available to the community via the peer review proposal process. We envision their primary use to be validation and characterization of exoplanet targets from the NASA, K2 and TESS missions and RV discovered exoplanets. Such targets will provide excellent follow-up candidates for both the WIYN and Gemini telescopes. We expect similar data quality in speckle imaging mode with the new instruments. Additionally, both cameras will have a wide-field mode and standard SDSS filters. They will be highly versatile instruments and it is that likely many other science programs will request time on the cameras. The limiting magnitude for speckle observations will remain around 13-14th at WIYN and 16-17th at Gemini, while wide-field, normal CCD imaging operation should be able to go to much fainter, providing usual CCD imaging and photometric capabilities. The instruments will also have high utility as scoring cameras for telescope engineering purposes, or other applications where high time resolution is needed. Instrument support will be provided, including a software pipeline that takes raw speckle data to fully reconstructed images.

  2. Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes.

    PubMed

    Lu, Wenlong; Chang, Ming; Chen, Po-Cheng; Luo, Wun-Mao

    2014-12-12

    Diffraction limit is usually a thorny problem in an optical inspection system. In this investigation, a model-based deconvolution technique was developed to recover diffraction-limited images, where images with sizes smaller than the diffraction limit could be recognized. Experiments were carried out with a traditional microscope at 200× magnification coupled with a halogen light source for a series of line width samples. The point spread function of the imaging optics was first obtained from an estimated model and then combined with a nonlinear deconvolution algorithm to calculate the full width at half maximum and reconstruct the line widths. Experimental results indicate that a measurement error below one pixel size of the measurement system is achievable. Accordingly, the target of nanoscale line width inspection based on a low cost and real-time image processing technique can be fulfilled, which greatly increases the ability of nanoscaling on optical microscopes.

  3. Breaking the diffraction limit by saturation in stimulated-Raman-scattering microscopy: A theoretical study

    NASA Astrophysics Data System (ADS)

    Gong, Li; Wang, Haifeng

    2014-07-01

    We present a theoretical investigation on the saturation of stimulated Raman scattering (SRS) and propose an application of it to break the diffraction limit in SRS microscopy. In our proposed scheme, a donut-shaped Stokes beam is used to saturate SRS at the rim of a focused Gaussian pump beam; thus the addition of another Gaussian Stokes beam can only induce additional stimulated Raman loss to the pump beam in a small area inside the donut-shaped beam. Resembling stimulated-emission-depletion microscopy, this method can significantly improve the lateral imaging resolution. Compared with the diffraction-limited resolution, theoretical simulations show that it may be possible to double the spatial resolution with a few TW/cm2 of laser intensity. Such super-resolution could greatly enhance the advantage of SRS microscopy for potential applications.

  4. Collective Effects in a Diffraction Limited Storage Ring

    SciTech Connect

    Nagaoka, Ryutaro; Bane, Karl L.F.

    2015-10-20

    Our paper gives an overview of collective effects that are likely to appear and possibly limit the performance in a diffraction-limited storage ring (DLSR) that stores a high-intensity ultra-low-emittance beam. Beam instabilities and other intensity-dependent effects that may significantly impact the machine performance are covered. The latter include beam-induced machine heating, Touschek scattering, intra-beam scattering, as well as incoherent tune shifts. The general trend that the efforts to achieve ultra-low emittance result in increasing the machine coupling impedance and the beam sensitivity to instability is reviewed. The nature of coupling impedance in a DLSR is described, followed by a series of potentially dangerous beam instabilities driven by the former, such as resistive-wall, TMCI (transverse mode coupling instability), head-tail and microwave instabilities. Additionally, beam-ion and CSR (coherent synchrotron radiation) instabilities are also treated. Means to fight against collective effects such as lengthening of the bunch with passive harmonic cavities and bunch-by-bunch transverse feedback are introduced. Numerical codes developed and used to evaluate the machine coupling impedance, as well as to simulate beam instability using the former as inputs are described.

  5. Collective Effects in a Diffraction Limited Storage Ring

    DOE PAGES

    Nagaoka, Ryutaro; Bane, Karl L.F.

    2015-10-20

    Our paper gives an overview of collective effects that are likely to appear and possibly limit the performance in a diffraction-limited storage ring (DLSR) that stores a high-intensity ultra-low-emittance beam. Beam instabilities and other intensity-dependent effects that may significantly impact the machine performance are covered. The latter include beam-induced machine heating, Touschek scattering, intra-beam scattering, as well as incoherent tune shifts. The general trend that the efforts to achieve ultra-low emittance result in increasing the machine coupling impedance and the beam sensitivity to instability is reviewed. The nature of coupling impedance in a DLSR is described, followed by a seriesmore » of potentially dangerous beam instabilities driven by the former, such as resistive-wall, TMCI (transverse mode coupling instability), head-tail and microwave instabilities. Additionally, beam-ion and CSR (coherent synchrotron radiation) instabilities are also treated. Means to fight against collective effects such as lengthening of the bunch with passive harmonic cavities and bunch-by-bunch transverse feedback are introduced. Numerical codes developed and used to evaluate the machine coupling impedance, as well as to simulate beam instability using the former as inputs are described.« less

  6. Computational-optical microscopy for 3D biological imaging beyond the diffraction limit

    NASA Astrophysics Data System (ADS)

    Grover, Ginni

    In recent years, super-resolution imaging has become an important fluorescent microscopy tool. It has enabled imaging of structures smaller than the optical diffraction limit with resolution less than 50 nm. Extension to high-resolution volume imaging has been achieved by integration with various optical techniques. In this thesis, development of a fluorescent microscope to enable high resolution, extended depth, three dimensional (3D) imaging is discussed; which is achieved by integration of computational methods with optical systems. In the first part of the thesis, point spread function (PSF) engineering for volume imaging is discussed. A class of PSFs, referred to as double-helix (DH) PSFs, is generated. The PSFs exhibit two focused spots in the image plane which rotate about the optical axis, encoding depth in rotation of the image. These PSFs extend the depth-of-field up to a factor of ˜5. Precision performance of the DH-PSFs, based on an information theoretical analysis, is compared with other 3D methods with conclusion that the DH-PSFs provide the best precision and the longest depth-of-field. Out of various possible DH-PSFs, a suitable PSF is obtained for super-resolution microscopy. The DH-PSFs are implemented in imaging systems, such as a microscope, with a special phase modulation at the pupil plane. Surface-relief elements which are polarization-insensitive and ˜90% light efficient are developed for phase modulation. The photon-efficient DH-PSF microscopes thus developed are used, along with optimal position estimation algorithms, for tracking and super-resolution imaging in 3D. Imaging at depths-of-field of up to 2.5 microm is achieved without focus scanning. Microtubules were imaged with 3D resolution of (6, 9, 39) nm, which is in close agreement with the theoretical limit. A quantitative study of co-localization of two proteins in volume was conducted in live bacteria. In the last part of the thesis practical aspects of the DH-PSF microscope are

  7. Fast time-resolved electrostatic force microscopy: Achieving sub-cycle time resolution

    NASA Astrophysics Data System (ADS)

    Karatay, Durmus U.; Harrison, Jeffrey S.; Glaz, Micah S.; Giridharagopal, Rajiv; Ginger, David S.

    2016-05-01

    The ability to measure microsecond- and nanosecond-scale local dynamics below the diffraction limit with widely available atomic force microscopy hardware would enable new scientific studies in fields ranging from biology to semiconductor physics. However, commercially available scanning-probe instruments typically offer the ability to measure dynamics only on time scales of milliseconds to seconds. Here, we describe in detail the implementation of fast time-resolved electrostatic force microscopy using an oscillating cantilever as a means to measure fast local dynamics following a perturbation to a sample. We show how the phase of the oscillating cantilever relative to the perturbation event is critical to achieving reliable sub-cycle time resolution. We explore how noise affects the achievable time resolution and present empirical guidelines for reducing noise and optimizing experimental parameters. Specifically, we show that reducing the noise on the cantilever by using photothermal excitation instead of piezoacoustic excitation further improves time resolution. We demonstrate the discrimination of signal rise times with time constants as fast as 10 ns, and simultaneous data acquisition and analysis for dramatically improved image acquisition times.

  8. Geometrical configurations of unphased diffraction-limited antennas in passive millimetre-wave imaging systems for concealed weapon detection

    NASA Astrophysics Data System (ADS)

    Serenelli, Roberto

    2004-12-01

    This paper analyzes simple imaging configurations to scan a human body, suitable as passive or active millimetre-wave imaging systems for concealed weapon detection (CWD). The first cylindrical configuration allows a 360 degrees scan: N unphased diffraction-limited antennas each of size L are placed on a circular support surrounding the subject (allowing scanning in the horizontal plane with N non-overlapping independent beams), and this circle is mechanically displaced over the whole body height. An analytical formula gives the maximum obtainable spatial resolution for different dimensions of the circular scanning device and operating frequencies, and the number of receivers achieving this optimal resolution. Constraints to be taken into account are diffraction, the usable total length of the circle, and the full coverage by the N beams over the subject, which is modelled as a cylinder with variable radius, coaxial with the scanning circle. Numerical calculations of system resolution are shown for different operating microwave (MW) and millimetre-wave (MMW) frequencies; in order to study off-axis performances, situations where the subject is not coaxial with the scanning device are also considered. For the case of a parallelepiped to be imaged instead of a cylinder, a linear array configuration is analyzed similarly to the circular one. A theoretical study is carried out to design other curved arrays, filled with unphased diffraction-limited antennas, for the imaging of linear subjects with finer resolution. Finally, the application of such configurations is considered for the design of active imaging systems, and different system architectures are discussed.

  9. An experimental apparatus for diffraction-limites soft x-ray nanofocusing

    SciTech Connect

    Merthe, Daniel; Goldberg, Kenneth; Yashchuk, Valeriy; Yuan, Sheng; McKinney, Wayne; Celestre, Richard; Mochi, Iacopo; Macdougall, James; Morrison, Gregory; Rakawa, Senajith; Anderson, Erik; Smith, Brian; Domning, Edward; Warwick, Tony; Padmore, Howard

    2011-10-21

    Realizing the experimental potential of high-brightness, next generation synchrotron and free-electron laser light sources requires the development of reflecting x-ray optics capable of wavefront preservation and high-resolution nano-focusing. At the Advanced Light Source (ALS) beamline 5.3.1, we are developing broadly applicable, high-accuracy, in situ, at-wavelength wavefront measurement techniques to surpass 100-nrad slope measurement accuracy for diffraction-limited Kirkpatrick-Baez (KB) mirrors. The at-wavelength methodology we are developing relies on a series of wavefront-sensing tests with increasing accuracy and sensitivity, including scanning-slit Hartmann tests, grating-based lateral shearing interferometry, and quantitative knife-edge testing. We describe the original experimental techniques and alignment methodology that have enabled us to optimally set a bendable KB mirror to achieve a focused, FWHM spot size of 150 nm, with 1 nm (1.24 keV) photons at 3.7 mrad numerical aperture. The predictions of wavefront measurement are confirmed by the knife-edge testing.The side-profiled elliptically bent mirror used in these one-dimensional focusing experiments was originally designed for a much different glancing angle and conjugate distances. This work demonstrates that high-accuracy, at-wavelength wavefront-slope feedback can be used to optimize the pitch, roll, and mirror-bending forces in situ, using procedures that are deterministic and repeatable.

  10. The First Diffraction-Limited Images from the W. M. Keck Telescope

    NASA Technical Reports Server (NTRS)

    Matthews, K.; Ghez, A. M.; Weinberger, A. J.; Neugebauer, G.

    1996-01-01

    The first diffraction limited, 0.05s resolution, images on the W. M. Keck Telescope have been obtained at a wavelength of 2.2 micrometers. These images were part of an experiment to test the suitability of the Keck Telescope for speckle imaging. In order to conduct this test, it was necessary to modify the pixel scale of the Keck facility Near Infrared Camera (NIRC) to optimally sample the spatial frequencies made available by the Keck telescope. The design and implementation of the external reimaging optics, which convert the standard fl25 beam from the secondary mirror to fl182, are described here. Techniques for reducing speckle data with field rotation on an alt-az telescope are also described. Three binary stars were observed in this experiment with separations as small as 0.05s. With only 100 frames of data on each, a dynamic range of at least 3.5 mag was achieved in all cases. These observations imply that a companion as faint as 14.5 mag at 2.2 micrometers could be detected around an 11th magnitude point source.

  11. Diffraction-limited high-finesse optical cavities

    SciTech Connect

    Kleckner, Dustin; Irvine, William T. M.; Oemrawsingh, Sumant S. R.; Bouwmeester, Dirk

    2010-04-15

    High-quality optical cavities with wavelength-sized end mirrors are important to the growing field of micro-optomechanical systems. We present a versatile method for calculating the modes of diffraction limited optical cavities and show that it can be used to determine the effect of a wide variety of cavity geometries and imperfections. Additionally, we show these calculations agree remarkably well with FDTD simulations for wavelength-sized optical modes, even though our method is based on the paraxial approximation.

  12. Diffraction Limited 3.15 Microns Cascade Diode Lasers

    DTIC Science & Technology

    2014-06-01

    carriers recycling by the cascade pumping . The narrow ridge 6- m-wide waveguides were defined by inductively coupled plasma (ICP) reactive ion etching...diffraction limited, diode lasers, cascade pumping REPORT DOCUMENTATION PAGE 11. SPONSOR/MONITOR’S REPORT NUMBER(S) 10. SPONSOR/MONITOR’S ACRONYM(S...of GaSb-based type-I QW diode lasers by utilizing cascade pumping scheme4. The carriers were recycled with 100% efficiency between two gain stages

  13. iLocater: A Diffraction-Limited Doppler Spectrometer for the Large Binocular Telescope

    NASA Astrophysics Data System (ADS)

    Crepp, Justin R.; Bechter, A.; Bechter, E.; Berg, M.; Carroll, J.; Collins, K.; Corpuz, T.; Ketterer, R.; Kielb, E.; Stoddard, R.; Eisner, J. A.; Gaudi, B. S.; Hinz, P.; Kratter, K. M.; Macela, G.; Quirrenbach, A.; Skrutskie, M. F.; Sozzetti, A.; Woodward, C. E.; Zhao, B.

    2014-01-01

    We are building an ultra-precise Doppler spectrometer for the Large Binocular Telescope (LBT) that operates at near-infrared wavelengths. The instrument, named iLocater, holds significant advantages over current and forth-coming Doppler designs. An R=110,000 spectrograph that operates in the Y-band, iLocater will receive a well-corrected beam from the LBT “extreme” adaptive optics system and use single-mode optical fibers to stabilize the instrument line spread function. With an input image 30 times smaller than comparable seeing-limited instruments (i.e., all Doppler radial velocity predecessors), iLocater will simultaneously achieve high spectral resolution, high spatial resolution, high throughput, and a compact optical design for low cost (affordable gratings). By working at the diffraction-limit, it is possible to circumvent, or ameliorate, many of the sources of noise common to seeing-limited spectrometers, including background contamination, thermal drifts, binary star interlopers, and pressure-induced changes in refractive index. Further, starlight received simultaneously from the LBT’s two separate telescope dishes may be used to monitor and remove internal systematic RV errors. iLocater will: identify "Earth-like" planets orbiting in the habitable-zone around nearby M-dwarf stars; perform the first systematic study of planet occurrence around binary stars as a function of their orbital separation; obtain the first spin-orbit orientation measurements of transiting terrestrial planets; and acquire essential follow-up observations for NASA's planned Transiting Exoplanet Survey Satellite (TESS) mission. In this poster, we present iLocater's design and science cases.

  14. Nanofocusing beyond the near-field diffraction limit via plasmonic Fano resonance.

    PubMed

    Song, Maowen; Wang, Changtao; Zhao, Zeyu; Pu, Mingbo; Liu, Ling; Zhang, Wei; Yu, Honglin; Luo, Xiangang

    2016-01-21

    The past decade has witnessed a great deal of optical systems designed for exceeding the Abbe's diffraction limit. Unfortunately, a deep subwavelength spot is obtained at the price of extremely short focal length, which is indeed a near-field diffraction limit that could rarely go beyond in the nanofocusing device. One method to mitigate such a problem is to set up a rapid oscillatory electromagnetic field that converges at the prescribed focus. However, abrupt modulation of phase and amplitude within a small fraction of a wavelength seems to be the main obstacle in the visible regime, aggravated by loss and plasmonic features that come into function. In this paper, we propose a periodically repeated ring-disk complementary structure to break the near-field diffraction limit via plasmonic Fano resonance, originating from the interference between the complex hybrid plasmon resonance and the continuum of propagating waves through the silver film. This plasmonic Fano resonance introduces a π phase jump in the adjacent channels and amplitude modulation to achieve radiationless electromagnetic interference. As a result, deep subwavelength spots as small as 0.0045λ(2) at 36 nm above the silver film have been numerically demonstrated. This plate holds promise for nanolithography, subdiffraction imaging and microscopy.

  15. FRIDA: diffraction-limited imaging and integral-field spectroscopy for the GTC

    NASA Astrophysics Data System (ADS)

    Watson, Alan M.; Acosta-Pulido, José A.; Álvarez-Núñez, Luis C.; Bringas-Rico, Vicente; Cardiel, Nicolás.; Cardona, Salvador; Chapa, Oscar; Díaz García, José Javier; Eikenberry, Stephen S.; Espejo, Carlos; Flores-Meza, Rubén. A.; Fuentes-Fernández, Jorge; Gallego, Jesús; Garcés Medina, José Leonardo; Garzón López, Francisco; Hammersley, Peter; Keiman, Carolina; Lara, Gerardo; López, José Alberto; López, Pablo L.; Lucero, Diana; Moreno Arce, Heidy; Pascual Ramirez, Sergio; Patrón Recio, Jesús; Prieto, Almudena; Rodríguez, Alberto José; Marco de la Rosa, José; Sánchez, Beatriz; Uribe, Jorge A.; Váldez Berriozabal, Francisco

    2016-08-01

    FRIDA is a diffraction-limited imager and integral-field spectrometer that is being built for the adaptive-optics focus of the Gran Telescopio Canarias. In imaging mode FRIDA will provide scales of 0.010, 0.020 and 0.040 arcsec/pixel and in IFS mode spectral resolutions of 1500, 4000 and 30,000. FRIDA is starting systems integration and is scheduled to complete fully integrated system tests at the laboratory by the end of 2017 and to be delivered to GTC shortly thereafter. In this contribution we present a summary of its design, fabrication, current status and potential scientific applications.

  16. Coherent total internal reflection dark-field microscopy: label-free imaging beyond the diffraction limit.

    PubMed

    von Olshausen, Philipp; Rohrbach, Alexander

    2013-10-15

    Coherent imaging is barely applicable in life-science microscopy due to multiple interference artifacts. Here, we show how these interferences can be used to improve image resolution and contrast. We present a dark-field microscopy technique with evanescent illumination via total internal reflection that delivers high-contrast images of coherently scattering samples. By incoherent averaging of multiple coherent images illuminated from different directions we can resolve image structures that remain unresolved by conventional (incoherent) fluorescence microscopy. We provide images of 190 nm beads revealing resolution beyond the diffraction limit and slightly increased object distances. An analytical model is introduced that accounts for the observed effects and which is confirmed by numerical simulations. Our approach may be a route to fast, label-free, super-resolution imaging in live-cell microscopy.

  17. Focusing metasurface quantum-cascade laser with a near diffraction-limited beam

    DOE PAGES

    Xu, Luyao; Chen, Daguan; Itoh, Tatsuo; ...

    2016-10-17

    A terahertz vertical-external-cavity surface-emitting-laser (VECSEL) is demonstrated using an active focusing reflectarray metasurface based on quantum-cascade gain material. The focusing effect enables a hemispherical cavity with flat optics, which exhibits higher geometric stability than a plano-plano cavity and a directive and circular near-diffraction limited Gaussian beam with M2 beam parameter as low as 1.3 and brightness of 1.86 × 106 Wsr–1m–2. As a result, this work initiates the potential of leveraging inhomogeneous metasurface and reflectarray designs to achieve high-power and high-brightness terahertz quantum-cascade VECSELs.

  18. Near-diffraction-limited diode end-pumped 2 µm Tm:YAG Innoslab laser

    NASA Astrophysics Data System (ADS)

    Huang, Haitao; Liu, Pian; Liu, Xuan; Wang, Hui; Jin, Lin; Shen, Deyuan

    2017-04-01

    A compact, near-diffraction-limited laser diode end-pumped 2 µm Tm:YAG Innoslab laser was demonstrated for the first time. A maximum output power of 36.4 W was achieved under the incident pump power of 184 W, corresponding to a slope efficiency of 23.4% with respect to the incident pump power. The beam quality factors M 2 were measured to be 1.44 in the stable direction and 1.23 in the unstable direction.

  19. Sub-diffraction-limit imaging using mode multiplexing

    NASA Astrophysics Data System (ADS)

    Wang, Nan; He, Jinping; Miyazaki, Jun; Tsurui, Hiromichi; Kobayashi, Takayoshi

    2015-10-01

    Simultaneous two-color subtraction microscopy using mode multiplexing is realized experimentally. The samples are irradiated with single laser diode at wavelength of 445 nm. Then the beam split laser spots generate separate solid and donut spatial modes and are multiplexed with modulators for simultaneous excitation. The produced fluorescence signals are back collected and further divided into two color bands with dichroic mirrors. Then they are detected with two photomultipliers and demultiplexed in four lock-in amplifiers. Four fluorescence images are recorded in every scan and resolution enhanced images are obtained in two color channels after applying the subtraction strategy. With this method, imaging results of microspheres stained with organic dyes and mesenteric lymph nodes of a mouse labeled with quantum dots (Q525/650) are realized. Improvement of 20% ~ 30% in resolving power of the two color channels compared with confocal microscopy is achieved in with corresponding subtraction factor of about 0.3.

  20. Achieving High Resolution Timer Events in Virtualized Environment

    PubMed Central

    Adamczyk, Blazej; Chydzinski, Andrzej

    2015-01-01

    Virtual Machine Monitors (VMM) have become popular in different application areas. Some applications may require to generate the timer events with high resolution and precision. This however may be challenging due to the complexity of VMMs. In this paper we focus on the timer functionality provided by five different VMMs—Xen, KVM, Qemu, VirtualBox and VMWare. Firstly, we evaluate resolutions and precisions of their timer events. Apparently, provided resolutions and precisions are far too low for some applications (e.g. networking applications with the quality of service). Then, using Xen virtualization we demonstrate the improved timer design that greatly enhances both the resolution and precision of achieved timer events. PMID:26177366

  1. Nanofocusing beyond the near-field diffraction limit via plasmonic Fano resonance

    NASA Astrophysics Data System (ADS)

    Song, Maowen; Wang, Changtao; Zhao, Zeyu; Pu, Mingbo; Liu, Ling; Zhang, Wei; Yu, Honglin; Luo, Xiangang

    2016-01-01

    The past decade has witnessed a great deal of optical systems designed for exceeding the Abbe's diffraction limit. Unfortunately, a deep subwavelength spot is obtained at the price of extremely short focal length, which is indeed a near-field diffraction limit that could rarely go beyond in the nanofocusing device. One method to mitigate such a problem is to set up a rapid oscillatory electromagnetic field that converges at the prescribed focus. However, abrupt modulation of phase and amplitude within a small fraction of a wavelength seems to be the main obstacle in the visible regime, aggravated by loss and plasmonic features that come into function. In this paper, we propose a periodically repeated ring-disk complementary structure to break the near-field diffraction limit via plasmonic Fano resonance, originating from the interference between the complex hybrid plasmon resonance and the continuum of propagating waves through the silver film. This plasmonic Fano resonance introduces a π phase jump in the adjacent channels and amplitude modulation to achieve radiationless electromagnetic interference. As a result, deep subwavelength spots as small as 0.0045λ2 at 36 nm above the silver film have been numerically demonstrated. This plate holds promise for nanolithography, subdiffraction imaging and microscopy.The past decade has witnessed a great deal of optical systems designed for exceeding the Abbe's diffraction limit. Unfortunately, a deep subwavelength spot is obtained at the price of extremely short focal length, which is indeed a near-field diffraction limit that could rarely go beyond in the nanofocusing device. One method to mitigate such a problem is to set up a rapid oscillatory electromagnetic field that converges at the prescribed focus. However, abrupt modulation of phase and amplitude within a small fraction of a wavelength seems to be the main obstacle in the visible regime, aggravated by loss and plasmonic features that come into function. In this

  2. At-wavelength interferometry of high-NA diffraction-limited EUV optics

    SciTech Connect

    Goldberg, Kenneth A.; Naulleau, Patrick; Rekawa, Senajith; Denham, Paul; Liddle, J. Alexander; Anderson, Erik; Jackson, Keith; Bokor, Jeffrey; Attwood, David

    2003-08-01

    Recent advances in all-reflective diffraction-limited optical systems designed for extreme ultraviolet (EUV) lithography have pushed numerical aperture (NA) values from 0.1 to 0.3, providing Rayleigh resolutions of 27-nm. Worldwide, several high-NA EUV optics are being deployed to serve in the development of advanced lithographic techniques required for EUV lithography, including the creation and testing of new, high-resolution photoresists. One such system is installed on an undulator beamline at Lawrence Berkeley National Laboratory's Advanced Light Source. Sub{angstrom}-accuracy optical testing and alignment techniques, developed for use with the previous generations of EUV lithographic optical systems, are being extended for use at high NA. Considerations for interferometer design and use are discussed.

  3. Plasmonic localized heating beyond the diffraction limit via magnetic polariton excitation

    NASA Astrophysics Data System (ADS)

    Alshehri, Hassan; Ying, Xiaoyan; Wang, Hao; Wang, Liping

    2016-09-01

    Optical localized heating in the nanoscale has recently attracted great attention due to its unique small hot spot size with high energy. However, the hot spot size is conventionally constrained by the diffraction limit. Plasmonic localized heating can provide solutions to this limitation in nanoscale patterning, cancer treatment, and data storage. Plasmonic approaches to overcome the diffraction limit in hot spot size have mainly utilized the excitation of surface plasmon or localized surface plasmon resonance. However, achieving plasmonic localized heating by the excitation of magnetic polariton has not been researched extensively yet. In this work, we numerically investigated the optical response of a nanoscale metamaterial composed of a gold nanowire array and a gold film separated by an ultrathin polymer spacer using ANSYS High Frequency Structural Simulator. A strong absorption peak at the wavelength of 760 nm was exhibited, and the underlying physical mechanism for the strong absorption was verified via the local electromagnetic field distribution to be magnetic resonance excitation. An inductor-capacitor circuit model was used to predict the magnetic resonance wavelength and compare with the numerical results for varied geometrical parameters. Volume loss density due to the strong local optical energy confinement was transferred as heat generation to an ANSYS thermal solver to obtain the local temperature profile. The steady state temperature profile shows an average temperature of 145 °C confined in a local area as small as 33 nm within the spacer, with a full-width at half-maximum of 50 nm along the x-direction. Moreover, the temperature rise from ambient drops to half its maximum value at a distance of 5 nm from the top of the spacer along the z-direction. This clearly demonstrates plasmonic localized heating beyond the diffraction limit via magnetic polariton excitation. Furthermore, the transient temperature profile shows that the system reached

  4. Efficient photonic reformatting of celestial light for diffraction-limited spectroscopy

    NASA Astrophysics Data System (ADS)

    MacLachlan, D. G.; Harris, R. J.; Gris-Sánchez, I.; Morris, T. J.; Choudhury, D.; Gendron, E.; Basden, A. G.; Spaleniak, I.; Arriola, A.; Birks, T. A.; Allington-Smith, J. R.; Thomson, R. R.

    2017-02-01

    The spectral resolution of a dispersive astronomical spectrograph is limited by the trade-off between throughput and the width of the entrance slit. Photonic guided wave transitions have been proposed as a route to bypass this trade-off, by enabling the efficient reformatting of incoherent seeing-limited light collected by the telescope into a linear array of single modes: a pseudo-slit which is highly multimode in one axis but diffraction-limited in the dispersion axis of the spectrograph. It is anticipated that the size of a single-object spectrograph fed with light in this manner would be essentially independent of the telescope aperture size. A further anticipated benefit is that such spectrographs would be free of `modal noise', a phenomenon that occurs in high-resolution multimode fibre-fed spectrographs due to the coherent nature of the telescope point spread function (PSF). We seek to address these aspects by integrating a multicore fibre photonic lantern with an ultrafast laser inscribed three-dimensional waveguide interconnect to spatially reformat the modes within the PSF into a diffraction-limited pseudo-slit. Using the CANARY adaptive optics (AO) demonstrator on the William Herschel Telescope, and 1530 ± 80 nm stellar light, the device exhibits a transmission of 47-53 per cent depending upon the mode of AO correction applied. We also show the advantage of using AO to couple light into such a device by sampling only the core of the CANARY PSF. This result underscores the possibility that a fully optimized guided-wave device can be used with AO to provide efficient spectroscopy at high spectral resolution.

  5. Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT

    NASA Astrophysics Data System (ADS)

    Hoyer, Patrick; de Medeiros, Gustavo; Balázs, Bálint; Norlin, Nils; Besir, Christina; Hanne, Janina; Kräusslich, Hans-Georg; Engelhardt, Johann; Sahl, Steffen J.; Hell, Stefan W.; Hufnagel, Lars

    2016-03-01

    We present a plane-scanning RESOLFT [reversible saturable/switchable optical (fluorescence) transitions] light-sheet (LS) nanoscope, which fundamentally overcomes the diffraction barrier in the axial direction via confinement of the fluorescent molecular state to a sheet of subdiffraction thickness around the focal plane. To this end, reversibly switchable fluorophores located right above and below the focal plane are transferred to a nonfluorescent state at each scanning step. LS-RESOLFT nanoscopy offers wide-field 3D imaging of living biological specimens with low light dose and axial resolution far beyond the diffraction barrier. We demonstrate optical sections that are thinner by 5-12-fold compared with their conventional diffraction-limited LS analogs.

  6. Focusing metasurface quantum-cascade laser with a near diffraction-limited beam

    SciTech Connect

    Xu, Luyao; Chen, Daguan; Itoh, Tatsuo; Reno, John L.; Williams, Benjamin S.

    2016-10-17

    A terahertz vertical-external-cavity surface-emitting-laser (VECSEL) is demonstrated using an active focusing reflectarray metasurface based on quantum-cascade gain material. The focusing effect enables a hemispherical cavity with flat optics, which exhibits higher geometric stability than a plano-plano cavity and a directive and circular near-diffraction limited Gaussian beam with M2 beam parameter as low as 1.3 and brightness of 1.86 × 106 Wsr–1m–2. As a result, this work initiates the potential of leveraging inhomogeneous metasurface and reflectarray designs to achieve high-power and high-brightness terahertz quantum-cascade VECSELs.

  7. Realization of periodic and quasiperiodic microstructures with sub-diffraction-limit feature sizes by far-field holographic lithography

    NASA Astrophysics Data System (ADS)

    Yang, Yi; Wang, Guo Ping

    2006-09-01

    The authors experimentally demonstrate a far-field holography for the realization of Ag nanoparticles-embedded periodic and quasiperiodic microstructures with feature sizes beyond the diffraction limit. Periodic cylindrical nanoshell arrays with about 240nm hole diameter and 12-fold symmetry quasiperiodic structures with 220nm feature sizes are achieved, respectively, by using a 632.8nm laser beam. Our results imply that conventional far-field optical technology is capable of fabricating nanostructures in modern micromanufacture.

  8. Quantum lithography beyond the diffraction limit via Rabi-oscillations

    NASA Astrophysics Data System (ADS)

    Liao, Zeyang; Al-Amri, Mohammad; Zubairy, M. Suhail

    2011-03-01

    We propose a quantum optical method to do the sub-wavelength lithography. Our method is similar to the traditional lithography but adding a critical step before dissociating the chemical bound of the photoresist. The subwavelength pattern is achieved by inducing the multi-Rabi-oscillation between the two atomic levels. The proposed method does not require multiphoton absorption and the entanglement of photons. This method is expected to be realizable using current technology. This work is supported by a grant from the Qatar National Research Fund (QNRF) under the NPRP project and a grant from the King Abdulaziz City for Science and Technology (KACST).

  9. Plasmonic local heating beyond diffraction limit by the excitation of magnetic polariton

    NASA Astrophysics Data System (ADS)

    Alshehri, Hassan; Wang, Hao; Ma, Yanchao; Wang, Liping

    2015-08-01

    In recent years, optical local heating in the nanoscale has attracted great attention due to its unique features of small hot spot size and high energy density. Plasmonic local heating can provide solutions to several challenges in data storage and cancer treatment. Research conducted in this field to achieve plasmonic local heating has mainly utilized the excitation of localized surface plasmon (LSP) or surface plasmon resonance (SPR). However, achieving plasmonic local heating by the excitation of magnetic polariton (MP) has not been researched extensively yet. We numerically investigate the optical response of a nanostructure composed of a gold nanowire on a gold surface separated by a polymer spacer using the ANSYS High Frequency Structural Simulator (HFSS). The structure exhibits a strong absorption peak at the wavelength of 750 nm, and the underlying physical mechanism is verified by the local electromagnetic field distribution to be the magnetic resonance excitation. By incorporating the volume loss density due to the strong local optical energy confinement as the heat generation, nanoscale temperature distribution within the structure is numerically obtained with a thermal solver after assigning proper boundary conditions. The results show a maximum temperature of 158.5°C confined in a local area on the order of 35 nm within the ultrathin polymer layer, which clearly demonstrates the plasmonic local heating effect beyond diffraction limit by excitation of MP.

  10. Diffraction-limited storage rings - a window to the science of tomorrow.

    PubMed

    Eriksson, Mikael; van der Veen, J Friso; Quitmann, Christoph

    2014-09-01

    This article summarizes the contributions in this special issue on Diffraction-Limited Storage Rings. It analyses the progress in accelerator technology enabling a significant increase in brightness and coherent fraction of the X-ray light provided by storage rings. With MAX IV and Sirius there are two facilities under construction that already exploit these advantages. Several other projects are in the design stage and these will probably enhance the performance further. To translate the progress in light source quality into new science requires similar progress in aspects such as optics, beamline technology, detectors and data analysis. The quality of new science will be limited by the weakest component in this value chain. Breakthroughs can be expected in high-resolution imaging, microscopy and spectroscopy. These techniques are relevant for many fields of science; for example, for the fundamental understanding of the properties of correlated electron materials, the development and characterization of materials for data and energy storage, environmental applications and bio-medicine.

  11. iLocater: a diffraction-limited Doppler spectrometer for the Large Binocular Telescope

    NASA Astrophysics Data System (ADS)

    Crepp, Justin R.; Crass, Jonathan; King, David; Bechter, Andrew; Bechter, Eric; Ketterer, Ryan; Reynolds, Robert; Hinz, Philip; Kopon, Derek; Cavalieri, David; Fantano, Louis; Koca, Corina; Onuma, Eleanya; Stapelfeldt, Karl; Thomes, Joseph; Wall, Sheila; Macenka, Steven; McGuire, James; Korniski, Ronald; Zugby, Leonard; Eisner, Joshua; Gaudi, B. S.; Hearty, Fred; Kratter, Kaitlin; Kuchner, Marc; Micela, Giusi; Nelson, Matthew; Pagano, Isabella; Quirrenbach, Andreas; Schwab, Christian; Skrutskie, Michael; Sozzetti, Alessandro; Woodward, Charles; Zhao, Bo

    2016-08-01

    We are developing a stable and precise spectrograph for the Large Binocular Telescope (LBT) named "iLocater." The instrument comprises three principal components: a cross-dispersed echelle spectrograph that operates in the YJ-bands (0.97-1.30 μm), a fiber-injection acquisition camera system, and a wavelength calibration unit. iLocater will deliver high spectral resolution (R 150,000-240,000) measurements that permit novel studies of stellar and substellar objects in the solar neighborhood including extrasolar planets. Unlike previous planet-finding instruments, which are seeing-limited, iLocater operates at the diffraction limit and uses single mode fibers to eliminate the effects of modal noise entirely. By receiving starlight from two 8.4m diameter telescopes that each use "extreme" adaptive optics (AO), iLocater shows promise to overcome the limitations that prevent existing instruments from generating sub-meter-per-second radial velocity (RV) precision. Although optimized for the characterization of low-mass planets using the Doppler technique, iLocater will also advance areas of research that involve crowded fields, line-blanketing, and weak absorption lines.

  12. Wavefront-correction for nearly diffraction-limited focusing of dual-color laser beams to high intensities.

    PubMed

    Zhao, Baozhen; Zhang, Jun; Chen, Shouyuan; Liu, Cheng; Golovin, Grigory; Banerjee, Sudeep; Brown, Kevin; Mills, Jared; Petersen, Chad; Umstadter, Donald

    2014-11-03

    We demonstrate wavefront correction of terawatt-peak-power laser beams at two distinct and well-separated wavelengths. Simultaneous near diffraction-limited focusability is achieved for both the fundamental (800 nm) and second harmonic (400 nm) of Ti:sapphire-amplified laser light. By comparing the relative effectiveness of various correction loops, the optimal ones are found. Simultaneous correction of both beams of different color relies on the linear proportionality between their wavefront aberrations. This method can enable two-color experiments at relativistic intensities.

  13. Characterization of Differential Toll-Like Receptor Responses below the Optical Diffraction Limit**

    PubMed Central

    Aaron, Jesse S.; Carson, Bryan D.; Timlin, Jerilyn A.

    2013-01-01

    Many membrane receptors are recruited to specific cell surface domains to form nanoscale clusters upon ligand activation. This step appears to be necessary to initiate signaling, including pathways in innate immune system activation. However, virulent pathogens such as Yersinia pestis (the causative agent of plague) are known to evade innate immune detection, in contrast to similar microbes (such as E. coli) that elicit a robust response. This disparity has been partly attributed to the structure of lipopolysaccharides (LPS) on the bacterial cell wall, which are recognized by the innate immune receptor TLR4. As such, we hypothesized that nanoscale differences would exist between the spatial clustering of TLR4 upon binding of LPS derived from Y. pestis and E. coli. Although optical imaging can provide exquisite details of the spatial organization of biomolecules, there is a mismatch between the scale at which receptor clustering occurs (<300 nm) and the optical diffraction limit (>400 nm). The last decade has seen the emergence of super-resolution imaging methods that effectively break the optical diffraction barrier to yield truly nanoscale information in intact biological samples. This study reports the first visualizations of TLR4 distributions on intact cells at image resolutions of <30 nm using a novel, dual-color stochastic optical reconstruction microscopy (STORM) technique. This methodology permits distinction between receptors containing bound LPS from those without at the nanoscale. Importantly, we also show that LPS derived from immuno-stimulatory bacteria resulted in significantly higher LPS-TLR4 cluster sizes and a nearly two-fold greater ligand/receptor colocalization as compared to immuno-evading LPS. PMID:22807232

  14. Diffraction-limited real-time terahertz imaging by optical frequency up-conversion in a DAST crystal.

    PubMed

    Fan, Shuzhen; Qi, Feng; Notake, Takashi; Nawata, Kouji; Takida, Yuma; Matsukawa, Takeshi; Minamide, Hiroaki

    2015-03-23

    Real-time terahertz (THz) wave imaging has wide applications in areas such as security, industry, biology, medicine, pharmacy, and the arts. This report describes real-time room-temperature THz imaging by nonlinear optical frequency up-conversion in an organic 4-dimethylamino-N'-methyl-4'-stilbazolium tosylate (DAST) crystal, with high resolution reaching the diffraction limit. THz-wave images were converted to the near infrared region and then captured using an InGaAs camera in a tandem imaging system. The resolution of the imaging system was analyzed. Diffraction and interference of THz wave were observed in the experiments. Videos are supplied to show the interference pattern variation that occurs with sample moving and tilting.

  15. Photoacoustic imaging beyond the acoustic diffraction-limit with dynamic speckle illumination and sparse joint support recovery

    NASA Astrophysics Data System (ADS)

    Hojman, Eliel; Chaigne, Thomas; Solomon, Oren; Gigan, Sylvain; Bossy, Emmanuel; Eldar, Yonina C.; Katz, Ori

    2017-03-01

    In deep tissue photoacoustic imaging the spatial resolution is inherently limited by the acoustic wavelength. Recently, it was demonstrated that it is possible to surpass the acoustic diffraction limit by analyzing fluctuations in a set of photoacoustic images obtained under unknown speckle illumination patterns. Here, we purpose an approach to boost reconstruction fidelity and resolution, while reducing the number of acquired images by utilizing a compressed sensing computational reconstruction framework. The approach takes into account prior knowledge of the system response and sparsity of the target structure. We provide proof of principle experiments of the approach and demonstrate that improved performance is obtained when both speckle fluctuations and object priors are used. We numerically study the expected performance as a function of the measurements signal to noise ratio and sample spatial-sparsity. The presented reconstruction framework can be applied to analyze existing photoacoustic experimental datasets containing dynamic fluctuations.

  16. Optically confined polarized resonance Raman studies in identifying crystalline orientation of sub-diffraction limited AlGaN nanostructure

    SciTech Connect

    Sivadasan, A. K. Patsha, Avinash; Dhara, Sandip

    2015-04-27

    An optical characterization tool of Raman spectroscopy with extremely weak scattering cross section tool is not popular to analyze scattered signal from a single nanostructure in the sub-diffraction regime. In this regard, plasmonic assisted characterization tools are only relevant in spectroscopic studies of nanoscale object in the sub-diffraction limit. We have reported polarized resonance Raman spectroscopic (RRS) studies with strong electron-phonon coupling to understand the crystalline orientation of a single AlGaN nanowire of diameter ∼100 nm. AlGaN nanowire is grown by chemical vapor deposition technique using the catalyst assisted vapor-liquid-solid process. The results are compared with the high resolution transmission electron microscopic analysis. As a matter of fact, optical confinement effect due to the dielectric contrast of nanowire with respect to that of surrounding media assisted with electron-phonon coupling of RRS is useful for the spectroscopic analysis in the sub-diffraction limit of 325 nm (λ/2N.A.) using an excitation wavelength (λ) of 325 nm and near ultraviolet 40× far field objective with a numerical aperture (N.A.) value of 0.50.

  17. Fluorescent microthermal imaging-theory and methodology for achieving high thermal resolution images

    SciTech Connect

    Barton, D.L.; Tangyunyong, P.

    1995-09-01

    The fluorescent microthermal imaging technique (FMI) involves coating a sample surface with an inorganic-based thin film that, upon exposure to UV light, emits temperature-dependent fluorescence. FMI offers the ability to create thermal maps of integrated circuits with a thermal resolution theoretically limited to 1 m{degrees}C and a spatial resolution which is diffraction-limited to 0.3 {mu}m. Even though the fluorescent microthermal imaging (FMI) technique has been around for more than a decade, many factors that can significantly affect the thermal image quality have not been systematically studied and characterized. After a brief review of FMI theory, we will present our recent results demonstrating for the first time three important factors that have a dramatic impact on the thermal quality and sensitivity of FMI. First, the limitations imparted by photon shot noise and improvement in the signal-to-noise ratio realized through signal averaging will be discussed. Second, ultraviolet bleaching, an unavoidable problem with FMI as it currently is performed, will be characterized to identify ways to minimize its effect. Finally, the impact of film dilution on thermal sensitivity will be discussed.

  18. Analysis of the scalability of diffraction-limited fiber lasers and amplifiers to high average power.

    PubMed

    Dawson, Jay W; Messerly, Michael J; Beach, Raymond J; Shverdin, Miroslav Y; Stappaerts, Eddy A; Sridharan, Arun K; Pax, Paul H; Heebner, John E; Siders, Craig W; Barty, C P J

    2008-08-18

    We analyze the scalability of diffraction-limited fiber lasers considering thermal, non-linear, damage and pump coupling limits as well as fiber mode field diameter (MFD) restrictions. We derive new general relationships based upon practical considerations. Our analysis shows that if the fiber's MFD could be increased arbitrarily, 36 kW of power could be obtained with diffraction-limited quality from a fiber laser or amplifier. This power limit is determined by thermal and non-linear limits that combine to prevent further power scaling, irrespective of increases in mode size. However, limits to the scaling of the MFD may restrict fiber lasers to lower output powers.

  19. Detecting single nanomagnet dynamics beyond the diffraction limit in varying magnetostatic environments

    NASA Astrophysics Data System (ADS)

    Liu, Z.; Brandt, R.; Yahagi, Y.; Hansen, B.; Harteneck, B.; Bokor, J.; Hawkins, A. R.; Schmidt, H.

    2011-01-01

    As areal bit density increases, characterizing individual magnetic bits within dense arrays becomes difficult with diffraction-limited optics. We demonstrate that dynamic magneto-optical detection breaks this diffraction limit if the characteristic behavior of a nanomagnet is sufficiently different from its neighbors'. We use far-field time-resolved Kerr microscopy to resolve the high-frequency magnetization dynamics of a single, small (Ø150 nm) nanomagnet within a low-frequency background from an array of large (Ø500 nm) magnets. We use this technique to observe and quantify the effects of magnetostatic interactions on the single magnet dynamics as the intermagnet spacing is varied.

  20. Fast exact scalar propagation for an in-line holographic microscopy on the diffraction limit.

    PubMed

    Kanka, M; Wuttig, A; Graulig, C; Riesenberg, R

    2010-01-15

    In lensless digital in-line holographic microscopy, currently applied fast reconstruction techniques use approximations limiting the usable NA for optical resolution. The computational effort for an exact scalar reconstruction with straightforward algorithms depends on the relation between the desired resolution and the given pixel pitch of the detector. So there is a trade-off between achievable image resolution and required computation time. We present an exact reconstruction algorithm that guaranties optimum resolution with affordable computation time. Experimental results show a realized NA of at least 0.62. A 1 megapixel hologram was reconstructed in about 1.5 s.

  1. Can Atomic Force Microscopy Achieve Atomic Resolution in Contact Mode?

    NASA Astrophysics Data System (ADS)

    Jarvis, M. R.; Pérez, Rubén; Payne, M. C.

    2001-02-01

    Atomic force microscopy operating in the contact mode is studied using total-energy pseudopotential calculations. It is shown that, in the case of a diamond tip and a diamond surface, it is possible for a tip terminated by a single atom to sustain forces in excess of 30 nN. It is also shown that imaging at atomic resolution may be limited by blunting of the tip during lateral scanning.

  2. The Surprising Outburst Behavior of Z Canis Majoris, and Resolving the Alpha Oph Companion Near the Diffraction limit

    NASA Astrophysics Data System (ADS)

    Hinkley, Sasha; Pope, Benjamin; Martinache, Frantz; Hillenbrand, Lynne; Kraus, Adam L.; Ireland, Michael; Oppenheimer, Ben R.; Rice, Emily L.; Monnier, John D.; Tuthill, Peter; Latyshev, Alexey

    2015-01-01

    We present recent high resolution Palomar and Keck observations on two intriguing binary star systems: Z Canis Majoris and Alpha Ophiuchus. We have obtained near-infrared Keck and Palomar photometry and spectra for each component of the Z Canis Majoris system, a very young binary composed of an FU Ori object and a Herbig Ae/Be object. Our high angular resolution photometry of this very young (~1 Myr) binary conclusively determines that the outburst was due solely to the embedded Herbig Ae/Be member, supporting results from earlier works. Further, our high-resolution K-band spectra during a quiescent phase definitively demonstrate that the 2.294 micron CO absorption feature seen in composite spectra of the system is due solely to the FU Ori component, while a prominent CO emission feature at the same wavelength, long suspected to be associated with the innermost regions of a circumstellar accretion disk, can be assigned to the Herbig Ae/Be member. These findings greatly clarify previous analyses of the origin of the CO emission in this complex system. In a different study, we detected the faint companion to the star Alpha Ophiuchus using the Palomar 5m Hale Telescope Adaptive Optics system combined with kernel phase interferometry, a recently-developed post-processing technique for high contrast imaging. The technique of kernel phase interferometry has never before been used to detect faint companions to nearby stars using ground-based observations. Our Palomar observations detect the Alpha Oph companion passing near its periastron point with separation of only ~130 miliarcseconds, close to the Palomar infrared diffraction limit. Alpha Oph is a particularly important binary system with the primary star rotating close to its breakup velocity. Thus, establishing the host star mass with high precision through dynamical orbital analysis is extremely valuable. This technique holds great promise for detecting high contrast objects at, or just inside, the formal

  3. High power broadband all fiber super-fluorescent source with linear polarization and near diffraction-limited beam quality.

    PubMed

    Ma, Pengfei; Huang, Long; Wang, Xiaolin; Zhou, Pu; Liu, Zejin

    2016-01-25

    In this manuscript, a high power broadband superfluorescent source (SFS) with linear polarization and near-diffraction-limited beam quality is achieved based on an ytterbium-doped (Yb-doped), all fiberized and polarization-maintained master oscillator power amplifier (MOPA) configuration. The MOPA structure generates a linearly polarized output power of 1427 W with a slope efficiency of 80% and a full width at half maximum (FWHM) of 11 nm, which is power scaled by an order of magnitude compared with the previously reported SFSs with linear polarization. In the experiment, both the polarization extinction ratio (PER) and beam quality (M(2) factor) are degraded little during the power scaling process. At maximal output power, the PER and M(2) factor are measured to be 19.1dB and 1.14, respectively. The root-mean-square (RMS) and peak-vale (PV) values of the power fluctuation at maximal output power are just 0.48% and within 3%, respectively. Further power scaling of the whole system is limited by the available pump sources. To the best of our knowledge, this is the first demonstration of kilowatt level broadband SFS with linear polarization and near-diffraction-limited beam quality.

  4. HYBRID simulations of diffraction-limited focusing with Kirkpatrick-Baez mirrors for a next-generation In Situ hard X-ray nanoprobe

    DOE PAGES

    Maser, Jorg; Shi, Xianbo; Reininger, Ruben; ...

    2016-02-22

    Next-generation hard X-ray nanoprobe beamlines such as the In Situ Nanoprobe (ISN) beamline being planned at the Advanced Photon Source aim at providing very high spatial resolution while also enabling very high focused flux, to study complex materials and devices using fast, multidimensional imaging across many length scales. The ISN will use diffractive optics to focus X-rays with a bandpass of ΔE/E = 10–4 into a focal spot of 20 nm or below. Reflective optics in Kirkpatrick-Baez geometry will be used to focus X-rays with a bandpass as large as ΔE/E = 10–2 into a focal spot of 50 nm.more » Diffraction-limited focusing with reflective optics is achieved by spatial filtering and use of a very long, vertically focusing mirror. Furthermore, to quantify the performance of the ISN beamline, we have simulated the propagation of both partially and fully coherent wavefronts from the undulator source, through the ISN beamline and into the mirror-based focal spot. Simulations were carried out using the recently developed software “HYBRID.”« less

  5. HYBRID simulations of diffraction-limited focusing with Kirkpatrick-Baez mirrors for a next-generation In Situ hard X-ray nanoprobe

    SciTech Connect

    Maser, Jorg; Shi, Xianbo; Reininger, Ruben; Lai, Barry; Vogt, Stefan

    2016-02-22

    Next-generation hard X-ray nanoprobe beamlines such as the In Situ Nanoprobe (ISN) beamline being planned at the Advanced Photon Source aim at providing very high spatial resolution while also enabling very high focused flux, to study complex materials and devices using fast, multidimensional imaging across many length scales. The ISN will use diffractive optics to focus X-rays with a bandpass of ΔE/E = 10–4 into a focal spot of 20 nm or below. Reflective optics in Kirkpatrick-Baez geometry will be used to focus X-rays with a bandpass as large as ΔE/E = 10–2 into a focal spot of 50 nm. Diffraction-limited focusing with reflective optics is achieved by spatial filtering and use of a very long, vertically focusing mirror. Furthermore, to quantify the performance of the ISN beamline, we have simulated the propagation of both partially and fully coherent wavefronts from the undulator source, through the ISN beamline and into the mirror-based focal spot. Simulations were carried out using the recently developed software “HYBRID.”

  6. HYBRID Simulations of Diffraction-Limited Focusing with Kirkpatrick-Baez Mirrors for a Next-Generation In Situ Hard X-ray Nanoprobe

    NASA Astrophysics Data System (ADS)

    Maser, Jörg; Shi, Xianbo; Reininger, Ruben; Lai, Barry; Vogt, Stefan

    2016-12-01

    Next-generation hard X-ray nanoprobe beamlines such as the In Situ Nanoprobe (ISN) beamline being planned at the Advanced Photon Source aim at providing very high spatial resolution while also enabling very high focused flux, to study complex materials and devices using fast, multidimensional imaging across many length scales. The ISN will use diffractive optics to focus X-rays with a bandpass of ∆ E/ E = 10-4 into a focal spot of 20 nm or below. Reflective optics in Kirkpatrick-Baez geometry will be used to focus X-rays with a bandpass as large as ∆ E/ E = 10-2 into a focal spot of 50 nm. Diffraction-limited focusing with reflective optics is achieved by spatial filtering and use of a very long, vertically focusing mirror. To quantify the performance of the ISN beamline, we have simulated the propagation of both partially and fully coherent wavefronts from the undulator source, through the ISN beamline and into the mirror-based focal spot. Simulations were carried out using the recently developed software " HYBRID."

  7. Super-resolving quantum radar: Coherent-state sources with homodyne detection suffice to beat the diffraction limit

    SciTech Connect

    Jiang, Kebei; Lee, Hwang; Gerry, Christopher C.; Dowling, Jonathan P.

    2013-11-21

    There has been much recent interest in quantum metrology for applications to sub-Raleigh ranging and remote sensing such as in quantum radar. For quantum radar, atmospheric absorption and diffraction rapidly degrades any actively transmitted quantum states of light, such as N00N states, so that for this high-loss regime the optimal strategy is to transmit coherent states of light, which suffer no worse loss than the linear Beer's law for classical radar attenuation, and which provide sensitivity at the shot-noise limit in the returned power. We show that coherent radar radiation sources, coupled with a quantum homodyne detection scheme, provide both longitudinal and angular super-resolution much below the Rayleigh diffraction limit, with sensitivity at shot-noise in terms of the detected photon power. Our approach provides a template for the development of a complete super-resolving quantum radar system with currently available technology.

  8. Fluorescent Saxitoxins for Live Cell Imaging of Single Voltage-Gated Sodium Ion Channels beyond the Optical Diffraction Limit

    PubMed Central

    Ondrus, Alison E.; Lee, Hsiao-lu D.; Iwanaga, Shigeki; Parsons, William H.; Andresen, Brian M.; Moerner, W.E.; Bois, J. Du

    2013-01-01

    SUMMARY A desire to better understand the role of voltagegated sodium channels (NaVs) in signal conduction and their dysregulation in specific disease states motivates the development of high precision tools for their study. Nature has evolved a collection of small molecule agents, including the shellfish poison (+)-saxitoxin, that bind to the extracellular pore of select NaV isoforms. As described in this report, de novo chemical synthesis has enabled the preparation of fluorescently labeled derivatives of (+)-saxitoxin, STX-Cy5, and STX-DCDHF, which display reversible binding to NaVs in live cells. Electrophysiology and confocal fluorescence microscopy studies confirm that these STX-based dyes function as potent and selective NaV labels. The utility of these probes is underscored in single-molecule and super-resolution imaging experiments, which reveal NaV distributions well beyond the optical diffraction limit in subcellular features such as neuritic spines and filopodia. PMID:22840778

  9. Instrument for Achieving High Angular Resolution on the Infrared Telescope

    NASA Technical Reports Server (NTRS)

    Hall, Donald N. B.

    1998-01-01

    Aberrations in stellar images caused by the atmosphere sets a significant limit on angular resolution in ground based astronomy. The largest of these aberrations is the image motion or wavefront tilt. Since the image motion is random it causes a blurring of the image, and this causes a blurring of the image from 0.3 arcseconds to about 0.7 arcseconds. The purpose of the tip-tilt project was to devise a system for the NASA Infrared Telescope Facility that would measure the image movement and correct it by rapidly tilting a mirror in two axes (tip and tilt). The system would involve building a CCD sensor package to measure the image motion, a new top end for the telescope to hold the tip-tilt mirror, a control system, and software. The system was designed to correct images for the facility camera, NSFCAM, and for the facility spectrometer, SPEX. Both of these instruments are equipped with a cold beamsplitter to feed the sensor package.

  10. Holographic fabricated photonic-crystal distributed-feedback quantum cascade laser with near-diffraction-limited beam quality.

    PubMed

    Lu, Quan-Yong; Zhang, Wei; Wang, Li-Jun; Liu, Jun-Qi; Li, Lu; Liu, Feng-Qi; Wang, Zhan-Guo

    2009-10-12

    We demonstrate the fabrication and characterization of photonic-crystal distributed-feedback quantum cascade laser emitting at 4.7 microm. The tilted rectangular-lattice PCDFB structure was defined using a multi-exposure of two-beam holographic lithography. The devices exhibit the near-diffraction-limited beam emission with the full width at half maximum of the far-field divergence angles about 4.5 degrees and 2.5 degrees for stripe widths of 55 microm and 95 microm, respectively. Single-mode emission with a side mode suppression ratio of approximately 20 dB is achieved in the temperature range (80-210 K). The single-facet output power is above 1 W for a 95 microm x 2.5 mm laser bar at 85 K in pulsed operation.

  11. Cryogenic, high power, near diffraction limited, Yb:YAG slab laser.

    PubMed

    Ganija, Miftar; Ottaway, David; Veitch, Peter; Munch, Jesper

    2013-03-25

    A cryogenic slab laser that is suitable for scaling to high power, while taking full advantage of the improved thermo-optical and thermo-mechanical properties of Yb:YAG at cryogenic temperatures is described. The laser uses a conduction cooled, end pumped, zigzag slab geometry resulting in a near diffraction limited, robust, power scalable design. The design and the initial characterization of the laser up to 200W are presented.

  12. Image contrast of diffraction-limited telescopes for circular incoherent sources of uniform radiance

    NASA Technical Reports Server (NTRS)

    Shackleford, W. L.

    1980-01-01

    A simple approximate formula is derived for the background intensity beyond the edge of the image of uniform incoherent circular light source relative to the irradiance near the center of the image. The analysis applies to diffraction-limited telescopes with or without central beam obscuration due to a secondary mirror. Scattering off optical surfaces is neglected. The analysis is expected to be most applicable to spaceborne IR telescopes, for which diffraction can be the major source of off-axis response.

  13. Super-Diffraction Limited Measurements through the Turbulent Atmosphere by Speckle Interferometry

    DTIC Science & Technology

    1990-02-22

    Extrasolar Planets; t 3.i oBrown Dwarfs; Diffraction Limited Imaging; Atmospheric Turbulence. 19. kBSTRACT (Continue on reverse if necessary and identify...which would enable the measurement of these parameters for large numbers of stars. Newly ) 20. DISTRIBUTION/AVAILABILITY OF ABSTRACT 21. ABSTRACT...nalyzed for spatial information and lend themselves to the followup determination of the atmospheric turbulence related parameters r0, r0 , and the

  14. High resolution telescope

    DOEpatents

    Massie, Norbert A.; Oster, Yale

    1992-01-01

    A large effective-aperture, low-cost optical telescope with diffraction-limited resolution enables ground-based observation of near-earth space objects. The telescope has a non-redundant, thinned-aperture array in a center-mount, single-structure space frame. It employs speckle interferometric imaging to achieve diffraction-limited resolution. The signal-to-noise ratio problem is mitigated by moving the wavelength of operation to the near-IR, and the image is sensed by a Silicon CCD. The steerable, single-structure array presents a constant pupil. The center-mount, radar-like mount enables low-earth orbit space objects to be tracked as well as increases stiffness of the space frame. In the preferred embodiment, the array has elemental telescopes with subaperture of 2.1 m in a circle-of-nine configuration. The telescope array has an effective aperture of 12 m which provides a diffraction-limited resolution of 0.02 arc seconds. Pathlength matching of the telescope array is maintained by an electro-optical system employing laser metrology. Speckle imaging relaxes pathlength matching tolerance by one order of magnitude as compared to phased arrays. Many features of the telescope contribute to substantial reduction in costs. These include eliminating the conventional protective dome and reducing on-site construction activites. The cost of the telescope scales with the first power of the aperture rather than its third power as in conventional telescopes.

  15. Wavelength scanning achieves pixel super-resolution in holographic on-chip microscopy

    NASA Astrophysics Data System (ADS)

    Luo, Wei; Göröcs, Zoltan; Zhang, Yibo; Feizi, Alborz; Greenbaum, Alon; Ozcan, Aydogan

    2016-03-01

    Lensfree holographic on-chip imaging is a potent solution for high-resolution and field-portable bright-field imaging over a wide field-of-view. Previous lensfree imaging approaches utilize a pixel super-resolution technique, which relies on sub-pixel lateral displacements between the lensfree diffraction patterns and the image sensor's pixel-array, to achieve sub-micron resolution under unit magnification using state-of-the-art CMOS imager chips, commonly used in e.g., mobile-phones. Here we report, for the first time, a wavelength scanning based pixel super-resolution technique in lensfree holographic imaging. We developed an iterative super-resolution algorithm, which generates high-resolution reconstructions of the specimen from low-resolution (i.e., under-sampled) diffraction patterns recorded at multiple wavelengths within a narrow spectral range (e.g., 10-30 nm). Compared with lateral shift-based pixel super-resolution, this wavelength scanning approach does not require any physical shifts in the imaging setup, and the resolution improvement is uniform in all directions across the sensor-array. Our wavelength scanning super-resolution approach can also be integrated with multi-height and/or multi-angle on-chip imaging techniques to obtain even higher resolution reconstructions. For example, using wavelength scanning together with multi-angle illumination, we achieved a halfpitch resolution of 250 nm, corresponding to a numerical aperture of 1. In addition to pixel super-resolution, the small scanning steps in wavelength also enable us to robustly unwrap phase, revealing the specimen's optical path length in our reconstructed images. We believe that this new wavelength scanning based pixel super-resolution approach can provide competitive microscopy solutions for high-resolution and field-portable imaging needs, potentially impacting tele-pathology applications in resource-limited-settings.

  16. Interferometric backward third harmonic generation microscopy for axial imaging with accuracy beyond the diffraction limit.

    PubMed

    Sandkuijl, Daaf; Kontenis, Lukas; Coelho, Nuno M; McCulloch, Christopher; Barzda, Virginijus

    2014-01-01

    A new nonlinear microscopy technique based on interference of backward-reflected third harmonic generation (I-THG) from multiple interfaces is presented. The technique is used to measure height variations or changes of a layer thickness with an accuracy of up to 5 nm. Height variations of a patterned glass surface and thickness variations of fibroblasts are visualized with the interferometric epi-THG microscope with an accuracy at least two orders of magnitude better than diffraction limit. The microscopy technique can be broadly applied for measuring distance variations between membranes or multilayer structures inside biological tissue and for surface height variation imaging.

  17. Diffraction Limited Imaging Spectroscopy of a Sgr A* Flare with OSIRIS

    NASA Astrophysics Data System (ADS)

    Krabbe, A.; Iserlohe, C.; Larkin, J. E.; Barczys, M.; McElwain, M.; Weiss, J.; Wright, S. A.; Quirrenbach, A.

    2006-12-01

    We present diffraction limited K-band integral field spectroscopy of a flare associated with Sgr A*. Prom the spectrum we determined the K-band spectral index of the pure flare emission to be (F(ν) propto να) of α = -2.6 ± 0.9. If we do not subtract the quiet state emission of SgrA*, then our spectral index is consistent with earlier observations of Ghez et al. (2005, ApJ, 635, in print). We compare our observations with other data already published and discuss the implications.

  18. Toward the diffraction limit with transmissive x-ray lenses in astronomy.

    PubMed

    Braig, Christoph; Predehl, Peter

    2012-07-10

    We develop an analytical approach to refractive, blazed diffractive, and achromatic x-ray lenses of scalable dimensions for energies from 1 to 20 keV. Based on the parabolic wave equation, their wideband imaging properties are compared and optimized for a given spectral range. Low-Z lens materials for massive cores and rugged alternatives, such as polycarbonate or Si for flat Fresnel components, are investigated with respect to their suitability for diffraction-limited high-energy astronomy. Properly designed "hybrid" combinations can serve as an approach to x-ray telescopes with an enhanced efficiency throughout the whole considered band, nearly regardless of their inherent absorption.

  19. Space infrared telescope facility wide field and diffraction limited array camera (IRAC)

    NASA Technical Reports Server (NTRS)

    Fazio, Giovanni G.

    1988-01-01

    The wide-field and diffraction limited array camera (IRAC) is capable of two-dimensional photometry in either a wide-field or diffraction-limited mode over the wavelength range from 2 to 30 microns with a possible extension to 120 microns. A low-doped indium antimonide detector was developed for 1.8 to 5.0 microns, detectors were tested and optimized for the entire 1.8 to 30 micron range, beamsplitters were developed and tested for the 1.8 to 30 micron range, and tradeoff studies of the camera's optical system performed. Data are presented on the performance of InSb, Si:In, Si:Ga, and Si:Sb array detectors bumpbonded to a multiplexed CMOS readout chip of the source-follower type at SIRTF operating backgrounds (equal to or less than 1 x 10 to the 8th ph/sq cm/sec) and temperature (4 to 12 K). Some results at higher temperatures are also presented for comparison to SIRTF temperature results. Data are also presented on the performance of IRAC beamsplitters at room temperature at both 0 and 45 deg angle of incidence and on the performance of the all-reflecting optical system baselined for the camera.

  20. A method for achieving super-resolved widefield CARS microscopy.

    PubMed

    Hajek, Kim M; Littleton, Brad; Turk, Douglas; McIntyre, Timothy J; Rubinsztein-Dunlop, Halina

    2010-08-30

    We propose a scheme for achieving widefield coherent anti-Stokes Raman scattering (CARS) microscopy images with sub-diffraction-limited resolution. This approach adds structured illumination to the widefield CARS configuration [Applied Physics Letters 84, 816 (2004)]. By capturing a number of images at different phases of the standing wave pattern, an image with up to three times the resolution of the original can be constructed. We develop a theoretical treatment of this system and perform numerical simulations for a typical CARS system, which indicate that resolutions around 120 nm are obtainable with the present scheme. As an imaging system, this method combines the advantages of sub-diffraction-limited resolution, endogenous contrast generation, and a wide field of view.

  1. Optical Imaging of Nonuniform Ferroelectricity and Strain at the Diffraction Limit

    PubMed Central

    Vlasin, Ondrej; Casals, Blai; Dix, Nico; Gutiérrez, Diego; Sánchez, Florencio; Herranz, Gervasi

    2015-01-01

    We have imaged optically the spatial distributions of ferroelectricity and piezoelectricity at the diffraction limit. Contributions to the birefringence from electro-optics –linked to ferroelectricity– as well as strain –arising from converse piezoelectric effects– have been recorded simultaneously in a BaTiO3 thin film. The concurrent recording of electro-optic and piezo-optic mappings revealed that, far from the ideal uniformity, the ferroelectric and piezoelectric responses were strikingly inhomogeneous, exhibiting significant fluctuations over the scale of the micrometer. The optical methods here described are appropriate to study the variations of these properties simultaneously, which are of great relevance when ferroelectrics are downscaled to small sizes for applications in data storage and processing. PMID:26522345

  2. Quantum statistical imaging of particles without restriction of the diffraction limit.

    PubMed

    Cui, Jin-Ming; Sun, Fang-Wen; Chen, Xiang-Dong; Gong, Zhao-Jun; Guo, Guang-Can

    2013-04-12

    A quantum measurement method based on the quantum nature of antibunching photon emission has been developed to detect single particles without the restriction of the diffraction limit. By simultaneously counting the single-photon and two-photon signals with fluorescence microscopy, the images of nearby nitrogen-vacancy centers in diamond at a distance of 8.5±2.4  nm have been successfully reconstructed. Also their axes information was optically obtained. This quantum statistical imaging technique, with a simple experimental setup, can also be easily generalized in the measuring and distinguishing of other physical properties with any overlapping, which shows high potential in future image and study of coupled quantum systems for quantum information techniques.

  3. Numerical prediction of minimum sub-diffraction-limit image generated by silver surface plasmon lenses.

    PubMed

    Fujii, Masafumi; Freude, Wolfgang; Leuthold, Juerg

    2008-12-08

    Sub-diffraction-limit imaging by the surface plasmon polariton (SPP) induced in thin metal film lenses has been analyzed numerically. The SPP images are deteriorated by interference of plasmon fields in layered metal-dielectric structures. To obtain a clear imaging capability, the reflection and the transmission property of evanescent waves in the layered structures has been investigated by the finite-difference time-domain (FDTD) method. For verification, a full 3-dimensional analysis of large-scale layered structures demonstrated sub-wavelength images similar to those obtained in the recently reported experiments. The analysis has been extended further to a lithography of nano-scale images to predict the minimum possible size of the images resolved by the silver thin film lenses.

  4. A novel method for spatially complex diffraction-limited photoactivation and photobleaching in living cells

    PubMed Central

    Shkryl, Vyacheslav M; Maxwell, Joshua T; Blatter, Lothar A

    2012-01-01

    Photoactivated probes have gained interest as experimental tools to study intracellular signalling pathways all the way to the molecular level. However technical limitations of the means to activate such compounds have put constraints on their use in spatially highly restricted subcellular areas. The Mosaic digital illumination system uses a high-speed array of individually addressable, tiltable micromirrors to direct continuous-wave laser light onto a specimen with diffraction-limited precision. The system, integrated into a Nikon A1R confocal microscope, was used to uncage Ca2+ or IP3 and conduct photobleaching experiments from multiple geometrically complex subcellular regions while simultaneously measuring [Ca2+]i with high-speed confocal imaging. PMID:22183727

  5. Three-dimensional array diffraction-limited foci from Greek ladders to generalized Fibonacci sequences.

    PubMed

    Zhang, Junyong

    2015-11-16

    Greek ladder is a technique for approximating Cn by rational numbers where n is a positive integer and C is a positive real number. For the classical Greek ladder, the value isC. Based on the continued fraction theory and algebraic equation, the classical Greek ladder in a special case can be reduced to the generalized Fibonacci sequence. By means of proper switching and binary, ternary or quaternary phase modulation, here we have successfully designed the various kinds of nano-photonic devices to produce three-dimensional array foci whose focusing properties satisfy the above mathematical characteristics. With this technology, the diffraction-limited array foci are freely designed or distributed under the requirement at the desired multiple focal planes.

  6. Effects of C(2)(n) on a vertically pointing diffraction-limited lidar.

    PubMed

    Schwiesow, R L

    1988-06-15

    Examples of different C(2)(n) profiles lead to substantially different profiles of lidar image radius in a study of the calculated performance of a diffraction-limited lidar system. The differences in image radii indicate the usefulness of a ground-based lidar for measurement of C(2)(n) profiles used to predict optical propagation phenomena. We conclude that the overall strength of the C(2)(n) profile and its general altitude dependence can be determined from inspection of the image radius profile. Approximate calculations of available and required SNRs show that a lidar with a telescope aperture of 0.5 m and a few pulses of ~1-J total transmitted energy will provide useful image radius data to an altitude of 20 km under daytime conditions. The weighting function for sensitivity of the fractional increase in image radius to changes of C(2)(n) on a logarithmic altitude scale is approximately constant with height.

  7. Achieving the resolution of the spectrograph of the 6m large Azimuthal telescope

    NASA Astrophysics Data System (ADS)

    Sazonenko, Dmitrii; Kukushkin, Dmitrii; Bakholdin, Alexey; Valyavin, Gennady

    2016-08-01

    Special Astrophysical Observatory of Russian Academy of Sciences (SAO RAS) creates a spectrograph with high spectral resolution for the 6-meter telescope. The spectrograph consists of a mobile unit located at the focus of the telescope's main mirror, a stationary part located under the telescope and optical fibers which transmit light from the mobile part to the stationary one. The spectral resolution of the stationary part should be R=100000. To achieve such a value, the scheme has two spectral elements, with cross-dispersion. The main spectral element is an echelle grating. The second spectral element is a prism with a diffraction grating on one facet.

  8. Implementation of parallel transmit beamforming using orthogonal frequency division multiplexing--achievable resolution and interbeam interference.

    PubMed

    Demi, Libertario; Viti, Jacopo; Kusters, Lieneke; Guidi, Francesco; Tortoli, Piero; Mischi, Massimo

    2013-11-01

    The speed of sound in the human body limits the achievable data acquisition rate of pulsed ultrasound scanners. To overcome this limitation, parallel beamforming techniques are used in ultrasound 2-D and 3-D imaging systems. Different parallel beamforming approaches have been proposed. They may be grouped into two major categories: parallel beamforming in reception and parallel beamforming in transmission. The first category is not optimal for harmonic imaging; the second category may be more easily applied to harmonic imaging. However, inter-beam interference represents an issue. To overcome these shortcomings and exploit the benefit of combining harmonic imaging and high data acquisition rate, a new approach has been recently presented which relies on orthogonal frequency division multiplexing (OFDM) to perform parallel beamforming in transmission. In this paper, parallel transmit beamforming using OFDM is implemented for the first time on an ultrasound scanner. An advanced open platform for ultrasound research is used to investigate the axial resolution and interbeam interference achievable with parallel transmit beamforming using OFDM. Both fundamental and second-harmonic imaging modalities have been considered. Results show that, for fundamental imaging, axial resolution in the order of 2 mm can be achieved in combination with interbeam interference in the order of -30 dB. For second-harmonic imaging, axial resolution in the order of 1 mm can be achieved in combination with interbeam interference in the order of -35 dB.

  9. Sub-Diffraction Limited Writing based on Laser Induced Periodic Surface Structures (LIPSS)

    PubMed Central

    He, Xiaolong; Datta, Anurup; Nam, Woongsik; Traverso, Luis M.; Xu, Xianfan

    2016-01-01

    Controlled fabrication of single and multiple nanostructures far below the diffraction limit using a method based on laser induced periodic surface structure (LIPSS) is presented. In typical LIPSS, multiple lines with a certain spatial periodicity, but often not well-aligned, were produced. In this work, well-controlled and aligned nanowires and nanogrooves with widths as small as 40 nm and 60 nm with desired orientation and length are fabricated. Moreover, single nanowire and nanogroove were fabricated based on the same mechanism for forming multiple, periodic structures. Combining numerical modeling and AFM/SEM analyses, it was found these nanostructures were formed through the interference between the incident laser radiation and the surface plasmons, the mechanism for forming LIPSS on a dielectric surface using a high power femtosecond laser. We expect that our method, in particular, the fabrication of single nanowires and nanogrooves could be a promising alternative for fabrication of nanoscale devices due to its simplicity, flexibility, and versatility. PMID:27721428

  10. Bispectrum speckle interferometry of the Red Rectangle: Diffraction-limited near-infrared images reconstructed from Keck telescope speckle data

    NASA Astrophysics Data System (ADS)

    Tuthill, P. G.; Men'shchikov, A. B.; Schertl, D.; Monnier, J. D.; Danchi, W. C.; Weigelt, G.

    2002-07-01

    We present new near-infrared (2.1-3.3 mu m) images of the Red Rectangle with unprecedented diffraction-limited angular resolutions of 46-68 mas; 4 times higher than that of the Hubble space telescope and almost a factor of two improvement over the previous 6 m SAO telecope speckle images presented by Men'shchikov et al. (\\cite{Men'shchikov_etal1998}). The new images, which were reconstructed from Keck telescope speckle data using the bispectrum speckle interferometry method, clearly show two bright lobes above and below the optically thick dark lane obscuring the central binary. X-shaped spikes, thought to trace the surface of a biconical flow, change the intensity distribution of the bright lobes, making them appear broadened or with an east-west double-peak in images with the highest resolution. The striking biconical appearance of the Red Rectangle is preserved on scales from 50 mas to 1 arcmin and from the visible (red) to at least 10 mu m, implying that large grains of at least several microns in size dominate scattering. The new images supplement previous 76 mas resolution speckle reconstructions at shorter wavelengths of 0.6-0.8 mu m (Osterbart et al. \\cite{Osterbart_etal1997}) and 0.7-2.2 mu m (Men'shchikov et al. \\cite{Men'shchikov_etal1998}), allowing a more detailed analysis of the famous bipolar nebula. The intensity distribution of the images is inconsistent with a flat disk geometry frequently used to model the bipolar nebulae. Instead, a geometrically thick torus-like density distribution with bipolar conical cavities is preferred. The extent of the bright lobes indicates that the dense torus has a diameter of >~ 100 AU, for an assumed distance of 330 pc. This torus may be the outer reaches of a flared thick disk tapering inwards to the central star, however such a density enhancement on the midplane is not strictly required to explain the narrow dark lane obscuring the central stars.

  11. Achieving selective interrogation and sub-wavelength resolution in thin plates with embedded metamaterial acoustic lenses

    SciTech Connect

    Semperlotti, F. Zhu, H.

    2014-08-07

    In this study, we present an approach to ultrasonic beam-forming and high resolution identification of acoustic sources having critical implications for applications such as structural health monitoring. The proposed concept is based on the design of dynamically tailored structural elements via embedded acoustic metamaterial lenses. This approach provides a completely new alternative to conventional phased-array technology enabling the formation of steerable and collimated (or focused) ultrasonic beams by exploiting a single transducer. Numerical results show that the ultrasonic beam can be steered by simply tuning the frequency of the excitation. Also, the embedded lens can be designed to achieve sub-wavelength resolution to clustered acoustic sources, which is a typical scenario encountered in incipient structural damage.

  12. Beyond the diffraction limit of optical/IR interferometers. II. Stellar parameters of rotating stars from differential phases

    NASA Astrophysics Data System (ADS)

    Hadjara, M.; Domiciano de Souza, A.; Vakili, F.; Jankov, S.; Millour, F.; Meilland, A.; Khorrami, Z.; Chelli, A.; Baffa, C.; Hofmann, K.-H.; Lagarde, S.; Robbe-Dubois, S.

    2014-09-01

    , PArot = 65.6° ± 5°, for Fomalhaut. They were found to be compatible with previously published values from differential phase and visibility measurements, while we were able to determine, for the first time, the inclination angle i of Fomalhaut (i = 90° ± 9°) and δ Aquilae (i = 81° ± 13°), and the rotation-axis position angle PArot of δ Aquilae. Conclusions: Beyond the theoretical diffraction limit of an interferometer (ratio of the wavelength to the baseline), spatial super resolution is well suited to systematically estimating the angular diameters of rotating stars and their fundamental parameters with a few sets of baselines and the Earth-rotation synthesis provided a high enough spectral resolution. Based on observations performed at the European Southern Observatory, Chile, under ESO AMBER-consortium GTO program IDs 084.D-0456 081.D-0293 and 082.C-0376.Figure 5 is available in electronic form at http://www.aanda.org

  13. Techniques for mass resolution improvement achieved by typical plasma mass analyzers: Modeling and simulations

    NASA Astrophysics Data System (ADS)

    Nicolaou, Georgios; Yamauchi, Masatoshi; Wieser, Martin; Barabash, Stas; Fedorov, Andrei

    2016-04-01

    Mass separation and particularly distinction between atomic ions and molecular ions are essential in understanding a wide range of plasma environments, with each consisted of different species with various properties. In this study we present the optimization results of light-weight (about 2 kg) magnetic mass analyzers with high g-factor for Rosetta (Ion Composition Analyser: ICA) and for Mars Express and Venus Express (Ion Mass Analyser: IMA). For the instrument's optimization we use SIMION, a 3D ion tracing software in which we can trace particle beams of several energies and directions, passing through the instrument's units. We first reproduced ICA and IMA results, which turned out to be different from simple models for low energy (< 100 eV). We then change the mechanical structure of several units of the instrument and we quantify the new mass resolution achieved with each change. Our goal is to find the optimal instrument's structure, which will allow us to achieve a proper mass resolution to distinguish atomic nitrogen from atomic oxygen for the purposes of a future magnetospheric mission.

  14. Efficient concept for generation of diffraction-limited green light by sum-frequency generation of spectrally combined tapered diode lasers.

    PubMed

    Müller, André; Jensen, Ole Bjarlin; Hasler, Karl-Heinz; Sumpf, Bernd; Erbert, Götz; Andersen, Peter E; Petersen, Paul Michael

    2012-09-15

    In order to increase the power of visible diode laser systems in an efficient manner, we propose spectral beam combining with subsequent sum-frequency generation. We show that this approach, in comparison with second harmonic generation of single emitters, can enhance the available power significantly. By combining two distributed Bragg reflector tapered diode lasers we achieve a 2.5-3.2 fold increase in power and a maximum of 3.9 W of diffraction-limited green light. At this power level, green diode laser systems have a high application potential, e.g., within the biomedical field. Our concept can be expanded combining multiple diode lasers to increase the power even further.

  15. Multiwavelength Diffraction-limited Imaging of the Evolved Carbon Star IRC +10216. II.

    NASA Astrophysics Data System (ADS)

    Tuthill, P. G.; Monnier, J. D.; Danchi, W. C.

    2005-05-01

    High angular resolution images of IRC +10216 taken at various bandpasses within the near-infrared H, K, and L bands are presented. The maps have the highest angular resolution yet recovered and were reconstructed from interferometric measurements obtained at the Keck I telescope in 1997 December and 1998 April, forming a subset of a seven-epoch monitoring program presented earlier by Tuthill and coworkers in Paper I. Systematic changes with observing wavelength are found and discussed in the context of present geometrical models for the circumstellar envelope. With these new high-resolution, multiwavelength data and contemporaneous photometry, we also revisit the hypothesis that the bright compact core of the nebula (component ``A'') marks the location of the central carbon star. We find that directly measured properties of the core (angular size, flux density, color temperature) are consistent with a reddened carbon star photosphere (line-of-sight τ2.2=5.3).

  16. Achieving behavioral control with millisecond resolution in a high-level programming environment.

    PubMed

    Asaad, Wael F; Eskandar, Emad N

    2008-08-30

    The creation of psychophysical tasks for the behavioral neurosciences has generally relied upon low-level software running on a limited range of hardware. Despite the availability of software that allows the coding of behavioral tasks in high-level programming environments, many researchers are still reluctant to trust the temporal accuracy and resolution of programs running in such environments, especially when they run atop non-real-time operating systems. Thus, the creation of behavioral paradigms has been slowed by the intricacy of the coding required and their dissemination across labs has been hampered by the various types of hardware needed. However, we demonstrate here that, when proper measures are taken to handle the various sources of temporal error, accuracy can be achieved at the 1 ms time-scale that is relevant for the alignment of behavioral and neural events.

  17. Mid-infrared spectroscopy beyond the diffraction limit via direct measurement of the photothermal effect.

    PubMed

    Katzenmeyer, A M; Holland, G; Chae, J; Band, A; Kjoller, K; Centrone, A

    2015-11-14

    An atomic force microscope equipped with temperature sensitive probes was used to measure locally the photothermal effect induced by IR light absorption. This novel instrument opens a pathway to correlated topographical, chemical composition, and thermal mapping with nanoscale resolution. Proof of principle demonstration is provided on polymers and plasmonic samples.

  18. Mid-infrared spectroscopy beyond the diffraction limit via direct measurement of the photothermal effect

    NASA Astrophysics Data System (ADS)

    Katzenmeyer, A. M.; Holland, G.; Chae, J.; Band, A.; Kjoller, K.; Centrone, A.

    2015-10-01

    An atomic force microscope equipped with temperature sensitive probes was used to measure locally the photothermal effect induced by IR light absorption. This novel instrument opens a pathway to correlated topographical, chemical composition, and thermal mapping with nanoscale resolution. Proof of principle demonstration is provided on polymers and plasmonic samples.An atomic force microscope equipped with temperature sensitive probes was used to measure locally the photothermal effect induced by IR light absorption. This novel instrument opens a pathway to correlated topographical, chemical composition, and thermal mapping with nanoscale resolution. Proof of principle demonstration is provided on polymers and plasmonic samples. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr04854k

  19. OBSERVATIONS OF BINARY STARS WITH THE DIFFERENTIAL SPECKLE SURVEY INSTRUMENT. III. MEASURES BELOW THE DIFFRACTION LIMIT OF THE WIYN TELESCOPE

    SciTech Connect

    Horch, Elliott P.; Van Altena, William F.; Howell, Steve B.; Sherry, William H.; Ciardi, David R. E-mail: william.vanaltena@yale.edu E-mail: wsherry@noao.edu

    2011-06-15

    In this paper, we study the ability of CCD- and electron-multiplying-CCD-based speckle imaging to obtain reliable astrometry and photometry of binary stars below the diffraction limit of the WIYN 3.5 m Telescope. We present a total of 120 measures of binary stars, 75 of which are below the diffraction limit. The measures are divided into two groups that have different measurement accuracy and precision. The first group is composed of standard speckle observations, that is, a sequence of speckle images taken in a single filter, while the second group consists of paired observations where the two observations are taken on the same observing run and in different filters. The more recent paired observations were taken simultaneously with the Differential Speckle Survey Instrument, which is a two-channel speckle imaging system. In comparing our results to the ephemeris positions of binaries with known orbits, we find that paired observations provide the opportunity to identify cases of systematic error in separation below the diffraction limit and after removing these from consideration, we obtain a linear measurement uncertainty of 3-4 mas. However, if observations are unpaired or if two observations taken in the same filter are paired, it becomes harder to identify cases of systematic error, presumably because the largest source of this error is residual atmospheric dispersion, which is color dependent. When observations are unpaired, we find that it is unwise to report separations below approximately 20 mas, as these are most susceptible to this effect. Using the final results obtained, we are able to update two older orbits in the literature and present preliminary orbits for three systems that were discovered by Hipparcos.

  20. Time-resolved photoemission apparatus achieving sub-20-meV energy resolution and high stability

    SciTech Connect

    Ishida, Y.; Togashi, T.; Yamamoto, K.; Tanaka, M.; Kiss, T.; Otsu, T.; Kobayashi, Y.; Shin, S.

    2014-12-15

    The paper describes a time- and angle-resolved photoemission apparatus consisting of a hemispherical analyzer and a pulsed laser source. We demonstrate 1.48-eV pump and 5.92-eV probe measurements at the ⩾10.5-meV and ⩾240-fs resolutions by use of fairly monochromatic 170-fs pulses delivered from a regeneratively amplified Ti:sapphire laser system operating typically at 250 kHz. The apparatus is capable to resolve the optically filled superconducting peak in the unoccupied states of a cuprate superconductor, Bi{sub 2}Sr{sub 2}CaCu{sub 2}O{sub 8+δ}. A dataset recorded on Bi(111) surface is also presented. Technical descriptions include the followings: A simple procedure to fine-tune the spatio-temporal overlap of the pump-and-probe beams and their diameters; achieving a long-term stability of the system that enables a normalization-free dataset acquisition; changing the repetition rate by utilizing acoustic optical modulator and frequency-division circuit.

  1. Numerical optimization of integrating cavities for diffraction-limited millimeter-wave bolometer arrays.

    PubMed

    Glenn, Jason; Chattopadhyay, Goutam; Edgington, Samantha F; Lange, Andrew E; Bock, James J; Mauskopf, Philip D; Lee, Adrian T

    2002-01-01

    Far-infrared to millimeter-wave bolometers designed to make astronomical observations are typically encased in integrating cavities at the termination of feedhorns or Winston cones. This photometer combination maximizes absorption of radiation, enables the absorber area to be minimized, and controls the directivity of absorption, thereby reducing susceptibility to stray light. In the next decade, arrays of hundreds of silicon nitride micromesh bolometers with planar architectures will be used in ground-based, suborbital, and orbital platforms for astronomy. The optimization of integrating cavity designs is required for achieving the highest possible sensitivity for these arrays. We report numerical simulations of the electromagnetic fields in integrating cavities with an infinite plane-parallel geometry formed by a solid reflecting backshort and the back surface of a feedhorn array block. Performance of this architecture for the bolometer array camera (Bolocam) for cosmology at a frequency of 214 GHz is investigated. We explore the sensitivity of absorption efficiency to absorber impedance and backshort location and the magnitude of leakage from cavities. The simulations are compared with experimental data from a room-temperature scale model and with the performance of Bolocam at a temperature of 300 mK. The main results of the simulations for Bolocam-type cavities are that (1) monochromatic absorptions as high as 95% are achievable with <1% cross talk between neighboring cavities, (2) the optimum absorber impedances are 400 ohms/sq, but with a broad maximum from approximately 150 to approximately 700 ohms/sq, and (3) maximum absorption is achieved with absorber diameters > or = 1.5 lambda. Good general agreement between the simulations and the experiments was found.

  2. Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging.

    PubMed

    Zeng, Zhiping; Chen, Xuanze; Wang, Hening; Huang, Ning; Shan, Chunyan; Zhang, Hao; Teng, Junlin; Xi, Peng

    2015-02-10

    Previous stochastic localization-based super-resolution techniques are largely limited by the labeling density and the fidelity to the morphology of specimen. We report on an optical super-resolution imaging scheme implementing joint tagging using multiple fluorescent blinking dyes associated with super-resolution optical fluctuation imaging (JT-SOFI), achieving ultra-high labeling density super-resolution imaging. To demonstrate the feasibility of JT-SOFI, quantum dots with different emission spectra were jointly labeled to the tubulin in COS7 cells, creating ultra-high density labeling. After analyzing and combining the fluorescence intermittency images emanating from spectrally resolved quantum dots, the microtubule networks are capable of being investigated with high fidelity and remarkably enhanced contrast at sub-diffraction resolution. The spectral separation also significantly decreased the frame number required for SOFI, enabling fast super-resolution microscopy through simultaneous data acquisition. As the joint-tagging scheme can decrease the labeling density in each spectral channel, thereby bring it closer to single-molecule state, we can faithfully reconstruct the continuous microtubule structure with high resolution through collection of only 100 frames per channel. The improved continuity of the microtubule structure is quantitatively validated with image skeletonization, thus demonstrating the advantage of JT-SOFI over other localization-based super-resolution methods.

  3. 160 W high-power, high-efficiency, near-diffraction-limited beam quality all-fiber picosecond pulse laser

    NASA Astrophysics Data System (ADS)

    Sun, Chang; Ge, Tingwu; An, Na; Cao, Kang; Wang, Zhiyong

    2016-10-01

    We experimentally demonstrate a high-power, high-efficiency, near-diffraction-limited beam quality all-fiber picosecond pulse laser, which consists of a passively mode-locked seed laser and three-stage master power amplifiers. A repetition frequency multiplier and a high Yb-doped gain fiber with shorter length are utilized in the laser system to suppress the nonlinear effects and reduce the pulse broadening caused by dispersion. Moreover, the homemade light mode controllers based on a coiling and tapering fiber technique and the active fiber of the amplifier with a relatively small mode area are adopted to improve the beam quality. In addition, by experimentally adjusting the active fiber length, the optical conversion efficiency of the overall laser system can be optimized. Eventually, a 160 W high-power, high-efficiency, near-diffraction-limited picosecond pulse fiber laser is obtained, with the beam quality factor M2 at 1.12 and an optical conversion efficiency of the system of 75%.

  4. Spin-flip resolution achieved with a one-proton self-excited oscillator

    NASA Astrophysics Data System (ADS)

    Guise, Nicholas Damien Sun-Wo

    In a Penning trap with an extremely large magnetic gradient, the axial frequency of a one-proton self-excited oscillator is resolved at the level of the shift from a proton spin flip. This sensitivity opens a possible path towards detection of single-proton spin flips, novel measurements of the proton and antiproton g-factors, and a stringent test of CPT invariance by comparing proton and antiproton magnetic moments at precision likely to be a million times higher than achieved to date. The central challenge of extending similar electron magnetic moment measurements to one proton is overcoming the substantially larger mass and weaker magnetic moment, which conspire to greatly reduce the frequency shift that signals a spin flip. Within a magnetic bottle gradient 50 times larger than used in the recent electron g-factor measurements, the proton spin-flip shift is still only 60 mHz out of a 553 kHz axial frequency. In such a large gradient, standard application of sideband cooling to reduce the magnetron radius changes the axial frequency by an amount greater than this spin-flip shift on average. Proton axial frequency resolution at the 60 mHz level is enabled by feedback techniques realized previously only with one electron. Self-excitation produces a narrow feature with large signal-to-noise, ideal for rapid frequency measurements at high precision. Unwanted effects of the strong magnetic gradient are minimized by axial and radial cooling. Feedback cooling is used to reduce the proton axial motion below the temperature of a damping resistor. Axial-magnetron sideband cooling of the undamped radial motion is then demonstrated to reach a 14 mK theoretical limit.

  5. Coherent beam combining of high power broad-area laser diode array with near diffraction limited beam quality and high power conversion efficiency.

    PubMed

    Liu, B; Braiman, Y

    2013-12-16

    We explored a path of achieving high quality phase-locking of broad-area laser diode (BALD) array that operates at high electrical to optical power conversion efficiency (PCE). We found that (a) improving single transverse mode control for each individual BALD, (b) employing global Talbot optical coupling among diodes, and (c) enhancing strength of optical coupling among diodes are key factors in achieving high quality phase-locking of high power BALD array. Subsequently, we redesigned and improved a V-shaped external Talbot cavity and employed low reflectivity anti-reflection (AR) coated, low-"smile" BALD array to meet these three important requirements. We demonstrated near-diffraction limit far-field coherent pattern with 19% PCE and 95% visibility. The far-field angle (full-width at half-maximum (FWHM)) of center lobe was measured as 1.5 diffraction angular limited with visibility of 99% for 5A injection current and 1.6 diffraction angular limited with visibility of 95% for 14A injection current. Power scaling of diode array is discussed.

  6. Fourier ring correlation as a resolution criterion for super-resolution microscopy.

    PubMed

    Banterle, Niccolò; Bui, Khanh Huy; Lemke, Edward A; Beck, Martin

    2013-09-01

    Optical nanoscopy techniques using localization based image reconstruction, also termed super-resolution microscopy (SRM), have become a standard tool to bypass the diffraction limit in fluorescence light microscopy. The localization precision measured for the detected fluorophores is commonly used to describe the maximal attainable resolution. However, this measure takes not all experimental factors, which impact onto the finally achieved resolution, into account. Several other methods to measure the resolution of super-resolved images were previously suggested, typically relying on intrinsic standards, such as molecular rulers, or on a priori knowledge about the specimen, e.g. its spatial frequency content. Here we show that Fourier ring correlation provides an easy-to-use, laboratory consistent standard for measuring the resolution of SRM images. We provide a freely available software tool that combines resolution measurement with image reconstruction.

  7. Sub-10 nm lateral spatial resolution in scanning capacitance microscopy achieved with solid platinum probes

    NASA Astrophysics Data System (ADS)

    Bussmann, E.; Williams, C. C.

    2004-02-01

    Sub-10 nm resolution can be obtained in scanning capacitance microscopy (SCM) if the probe tip is approximately of the same size. Such resolution is observed, although infrequently, with present commercially available probes. To acquire routine sub-10 nm resolution, a solid Pt metal probe has been developed with a sub-10 nm tip radius. The probe is demonstrated by SCM imaging on a cross-sectioned 70 nm gatelength field-effect transistor (FET), a shallow implant (n+/p, 24 nm junction depth), and an epitaxial staircase (p, ˜75 nm steps). Sub-10 nm resolution is demonstrated on the FET device over the abrupt meeting between a silicon-on-insulator oxide layer and a neighboring Si region. Comparable resolution is observed on the implant structure, and quantitative SCM dopant profiling is performed on it with sub-10 nm accuracy. Finally, the epitaxial staircase structure is quantitatively profiled demonstrating the accuracy obtained in quantitative profiling with the tips.

  8. Single Molecules, Cells, and Super-Resolution Optics (Nobel Lecture).

    PubMed

    Betzig, Eric

    2015-07-06

    The resolution of a microscope is determined by the diffraction limit in classical microscopy, whereby objects that are separated by half a wavelength can no longer be visually separated. To go below the diffraction limit required several tricks and discoveries. In his Nobel Lecture, E. Betzig describes the developments that have led to modern super high-resolution microscopy.

  9. Dynamic diffraction-limited light-coupling of 3D-maneuvered wave-guided optical waveguides.

    PubMed

    Villangca, Mark; Bañas, Andrew; Palima, Darwin; Glückstad, Jesper

    2014-07-28

    We have previously proposed and demonstrated the targeted-light delivery capability of wave-guided optical waveguides (WOWs). As the WOWs are maneuvered in 3D space, it is important to maintain efficient light coupling through the waveguides within their operating volume. We propose the use of dynamic diffractive techniques to create diffraction-limited spots that will track and couple to the WOWs during operation. This is done by using a spatial light modulator to encode the necessary diffractive phase patterns to generate the multiple and dynamic coupling spots. The method is initially tested for a single WOW and we have experimentally demonstrated dynamic tracking and coupling for both lateral and axial displacements.

  10. X-ray optics simulation and beamline design using a hybrid method: diffraction-limited focusing mirrors

    NASA Astrophysics Data System (ADS)

    Shi, Xianbo; Reininger, Ruben; Sánchez del Río, Manuel; Qian, Jun; Assoufid, Lahsen

    2014-09-01

    A hybrid method combining ray-tracing and wavefront propagation was recently developed for X-ray optics simulation and beamline design optimization. One major application of the hybrid method is its ability to assess the effects of figure errors on the performance of focusing mirrors. In the present work, focusing profiles of mirrors with different figure errors are simulated using three available wave optics methods: the hybrid code based on the Fourier optics approach, the stationary phase approximation and a technique based on the direct Fresnel-Kirchhoff diffraction integral. The advantages and limitations of each wave optics method are discussed. We also present simulations performed using the figure errors of an elliptical cylinder mirror measured at APS using microstitching interferometry. These results show that the hybrid method provides accurate and quick evaluation of the expected mirror performance making it a useful tool for designing diffraction-limited focusing beamlines.

  11. Large deflection angle, high-power adaptive fiber optics collimator with preserved near-diffraction-limited beam quality.

    PubMed

    Zhi, Dong; Ma, Yanxing; Chen, Zilun; Wang, Xiaolin; Zhou, Pu; Si, Lei

    2016-05-15

    We report on the development of a monolithic adaptive fiber optics collimator, with a large deflection angle and preserved near-diffraction-limited beam quality, that has been tested at a maximal output power at the 300 W level. Additionally, a new measurement method of beam quality (M2 factor) is developed. Experimental results show that the deflection angle of the collimated beam is in the range of 0-0.27 mrad in the X direction and 0-0.19 mrad in the Y direction. The effective working frequency of the device is about 710 Hz. By employing the new measurement method of the M2 factor, we calculate that the beam quality is Mx2=1.35 and My2=1.24, which is in agreement with the result from the beam propagation analyzer and is preserved well with the increasing output power.

  12. Optimizing the lattice design of a diffraction-limited storage ring with a rational combination of particle swarm and genetic algorithms

    NASA Astrophysics Data System (ADS)

    Jiao, Yi; Xu, Gang

    2017-02-01

    In the lattice design of a diffraction-limited storage ring (DLSR) consisting of compact multi-bend achromats (MBAs), it is challenging to simultaneously achieve an ultralow emittance and a satisfactory nonlinear performance, due to extremely large nonlinearities and limited tuning ranges of the element parameters. Nevertheless, in this paper we show that the potential of a DLSR design can be explored with a successive and iterative implementation of the multi-objective particle swarm optimization (MOPSO) and multi-objective genetic algorithm (MOGA). For the High Energy Photon Source, a planned kilometer-scale DLSR, optimizations indicate that it is feasible to attain a natural emittance of about 50 pm·rad, and simultaneously realize a sufficient ring acceptance for on-axis longitudinal injection, by using a hybrid MBA lattice. In particular, this study demonstrates that a rational combination of the MOPSO and MOGA is more effective than either of them alone, in approaching the true global optima of an explorative multi-objective problem with many optimizing variables and local optima. Supported by NSFC (11475202, 11405187) and Youth Innovation Promotion Association CAS (2015009)

  13. Teachers, Technology and Training: A New Year's Resolution for 2006: Closing the Achievement Gap

    ERIC Educational Resources Information Center

    Donlevy, Jim

    2006-01-01

    Recognizing the glaring discrepancies in outcomes for many poor and minority children, the New York State Regents hosted an Education Summit in November 2005 with the theme of Closing the Achievement Gap. Leaders in business, government and education were in attendance and rallied to more thoroughly understand the achievement gap and produce…

  14. Achieving atomic resolution magnetic dichroism by controlling the phase symmetry of an electron probe

    DOE PAGES

    Rusz, Jan; Idrobo, Juan -Carlos; Bhowmick, Somnath

    2014-09-30

    The calculations presented here reveal that an electron probe carrying orbital angular momentum is just a particular case of a wider class of electron beams that can be used to measure electron magnetic circular dichroism (EMCD) with atomic resolution. It is possible to obtain an EMCD signal with atomic resolution by simply breaking the symmetry of the electron probe phase front using the aberration-corrected optics of a scanning transmission electron microscope. The probe’s required phase distribution depends on the sample’s magnetic symmetry and crystal structure. The calculations indicate that EMCD signals that use the electron probe’s phase are as strongmore » as those obtained by nanodiffraction methods.« less

  15. Achieving atomic resolution magnetic dichroism by controlling the phase symmetry of an electron probe

    SciTech Connect

    Rusz, Jan; Idrobo, Juan -Carlos; Bhowmick, Somnath

    2014-09-30

    The calculations presented here reveal that an electron probe carrying orbital angular momentum is just a particular case of a wider class of electron beams that can be used to measure electron magnetic circular dichroism (EMCD) with atomic resolution. It is possible to obtain an EMCD signal with atomic resolution by simply breaking the symmetry of the electron probe phase front using the aberration-corrected optics of a scanning transmission electron microscope. The probe’s required phase distribution depends on the sample’s magnetic symmetry and crystal structure. The calculations indicate that EMCD signals that use the electron probe’s phase are as strong as those obtained by nanodiffraction methods.

  16. Localized tip enhanced Raman spectroscopic study of impurity incorporated single GaN nanowire in the sub-diffraction limit

    SciTech Connect

    Patsha, Avinash E-mail: dhara@igcar.gov.in; Dhara, Sandip; Tyagi, A. K.

    2015-09-21

    The localized effect of impurities in single GaN nanowires in the sub-diffraction limit is reported using the study of lattice vibrational modes in the evanescent field of Au nanoparticle assisted tip enhanced Raman spectroscopy (TERS). GaN nanowires with the O impurity and the Mg dopants were grown by the chemical vapor deposition technique in the catalyst assisted vapor-liquid-solid process. Symmetry allowed Raman modes of wurtzite GaN are observed for undoped and doped nanowires. Unusually very strong intensity of the non-zone center zone boundary mode is observed for the TERS studies of both the undoped and the Mg doped GaN single nanowires. Surface optical mode of A{sub 1} symmetry is also observed for both the undoped and the Mg doped GaN samples. A strong coupling of longitudinal optical (LO) phonons with free electrons, however, is reported only in the O rich single nanowires with the asymmetric A{sub 1}(LO) mode. Study of the local vibration mode shows the presence of Mg as dopant in the single GaN nanowires.

  17. Investigation of stimulated raman scattering using short-pulse diffraction limited laser beam near the instability threshold

    SciTech Connect

    Kline, John L; Montgomery, David S; Flippo, Kirk A; Rose, Harvey A; Yin, L; Albright, B J; Johnson, R P; Shimada, T; Bowers, K; Rousseaux, C; Tassin, V; Baton, S D; Amiranoff, F; Hardin, R A

    2008-01-01

    Short pulse laser plasma interaction experiments using diffraction limited beams provide an excellent platform to investigate the fundamental physics of Stimulated Raman Scattering. Detailed understanding of these laser plasma instabilities impacts the current inertial confinement fusion ignition designs and could potentially impact fast ignition when higher energy lasers are used with longer pulse durations ( > 1 kJ and> 1 ps). Using short laser pulses, experiments can be modeled over the entire interaction time of the laser using particle-in-cell codes to validate our understanding quantitatively. Experiments have been conducted at the Trident laser facility and the LULI (Laboratoire pour l'Utilisation des Lasers Intenses) to investigate stimulated Raman scattering near the threshold of the instability using 527 nm and 1059 nm laser light respectively with 1.5-3.0 ps pulses. In both experiments, the interaction beam was focused into a pre-ionized He gas-jet plasma. Measurements of the reflectivity as a function of intensity and k{lambda}{sub D} were completed at the Trident laser facility. At LULI, a 300 fs Thomson scattering probe is used to directly measure the density fluctuations of the driven electron plasma and ion acoustic waves. Work is currently underway comparing the results of the experiments with simulations using the VPIC [K. J. Bowers, et at., Phys. Plasmas, 15 055703 (2008)] particle-in-cell code. Details of the experimental results are presented in this manuscript.

  18. Enhancement of low-quality reconstructed digital hologram images based on frequency extrapolation of large objects under the diffraction limit

    NASA Astrophysics Data System (ADS)

    Liu, Ning; Li, Weiliang; Zhao, Dongxue

    2016-06-01

    During the reconstruction of a digital hologram, the reconstructed image is usually degraded by speckle noise, which makes it hard to observe the original object pattern. In this paper, a new reconstructed image enhancement method is proposed, which first reduces the speckle noise using an adaptive Gaussian filter, then calculates the high frequencies that belong to the object pattern based on a frequency extrapolation strategy. The proposed frequency extrapolation first calculates the frequency spectrum of the Fourier-filtered image, which is originally reconstructed from the +1 order of the hologram, and then gives the initial parameters for an iterative solution. The analytic iteration is implemented by continuous gradient threshold convergence to estimate the image level and vertical gradient information. The predicted spectrum is acquired through the analytical iteration of the original spectrum and gradient spectrum analysis. Finally, the reconstructed spectrum of the restoration image is acquired from the synthetic correction of the original spectrum using the predicted gradient spectrum. We conducted our experiment very close to the diffraction limit and used low-quality equipment to prove the feasibility of our method. Detailed analysis and figure demonstrations are presented in the paper.

  19. Enhancement of low quality reconstructed digital hologram images based on frequency extrapolation of large objects under the diffraction limit

    NASA Astrophysics Data System (ADS)

    Liu, Ning; Chen, Xiaohong; Yang, Chao

    2016-11-01

    During the reconstruction of a digital hologram, the reconstructed image is usually degraded by speckle noise, which makes it hard to observe the original object pattern. In this paper, a new reconstructed image enhancement method is proposed, which first reduces the speckle noise using an adaptive Gaussian filter, then calculates the high frequencies that belong to the object pattern based on a frequency extrapolation strategy. The proposed frequency extrapolation first calculates the frequency spectrum of the Fourier-filtered image, which is originally reconstructed from the +1 order of the hologram, and then gives the initial parameters for an iterative solution. The analytic iteration is implemented by continuous gradient threshold convergence to estimate the image level and vertical gradient information. The predicted spectrum is acquired through the analytical iteration of the original spectrum and gradient spectrum analysis. Finally, the reconstructed spectrum of the restoration image is acquired from the synthetic correction of the original spectrum using the predicted gradient spectrum. We conducted our experiment very close to the diffraction limit and used low quality equipment to prove the feasibility of our method. Detailed analysis and figure demonstrations are presented in the paper.

  20. The close circumstellar environment of Betelgeuse. II. Diffraction-limited spectro-imaging from 7.76 to 19.50 μm with VLT/VISIR

    NASA Astrophysics Data System (ADS)

    Kervella, P.; Perrin, G.; Chiavassa, A.; Ridgway, S. T.; Cami, J.; Haubois, X.; Verhoelst, T.

    2011-07-01

    Context. Mass-loss occurring in red supergiants (RSGs) is a major contributor to the enrichment of the interstellar medium in dust and molecules. The physical mechanism of this mass loss is however relatively poorly known. Betelgeuse is the nearest RSG, and as such a prime object for high angular resolution observations of its surface (by interferometry) and close circumstellar environment. Aims: The goal of our program is to understand how the material expelled from Betelgeuse is transported from its surface to the interstellar medium, and how it evolves chemically in this process. Methods: We obtained diffraction-limited images of Betelgeuse and a calibrator (Aldebaran) in six filters in the N band (7.76 to 12.81 μm) and two filters in the Q band (17.65 and 19.50 μm), using the VLT/VISIR instrument. Results: Our images show a bright, extended and complex circumstellar envelope at all wavelengths. It is particularly prominent longwards of ≈ 9-10 μm, pointing at the presence of O-rich dust, such as silicates or alumina. A partial circular shell is observed between 0.5 and 1.0″ from the star, and could correspond to the inner radius of the dust envelope. Several knots and filamentary structures are identified in the nebula. One of the knots, located at a distance of 0.9″ west of the star, is particularly bright and compact. Conclusions: The circumstellar envelope around Betelgeuse extends at least up to several tens of stellar radii. Its relatively high degree of clumpiness indicates an inhomogeneous spatial distribution of the material lost by the star. Its extension corresponds to an important intermediate scale, where most of the dust is probably formed, between the hot and compact gaseous envelope observed previously in the near infrared and the interstellar medium. Based on observations made with ESO telescopes at Paranal Observatory, under ESO DDT program 286.D-5007(A).

  1. Achieving High Resolution Ion Mobility Separations Using Traveling Waves in Compact Multiturn Structures for Lossless Ion Manipulations

    PubMed Central

    Ibrahim, Yehia M.; Deng, Liulin; Zheng, Xueyun; Webb, Ian K.; Anderson, Gordon A.; Prost, Spencer A.; Norheim, Randolph V.; Tolmachev, Aleksey V.; Baker, Erin S.; Smith, Richard D.

    2017-01-01

    We report on ion mobility (IM) separations achievable using traveling waves (TW) in a Structures for Lossless Ion Manipulations (SLIM) module having a 44 cm path length and 16 90° turns. The performance of the TW-SLIM module was evaluated for ion transmission and IM separations with different RF, TW parameters, and SLIM surface gaps in conjunction with mass spectrometry. In this work, TWs were created by the transient and dynamic application of DC potentials. The module demonstrated highly robust performance and, even with 16 closely spaced turns, achieving IM resolution performance and ion transmission comparable to a similar straight path module. We found an IM peak capacity of ~31 and peak generation rate of 780 s−1 for TW speeds of ~80 m/s using the current multi-turn TW-SLIM module. The separations achieved for isomers of peptides and tetrasaccharides were found to be comparable to those from a ~0.9-m drift tube-based IM-MS platform operated at the same pressure (4 Torr). The combined attributes of flexible design, low voltage requirements and lossless ion transmission through multiple turns for the present TW-SLIM module provides a basis for SLIM devices capable of achieving much greater IM resolution via greatly extended ion path lengths and using compact serpentine designs. PMID:27479234

  2. Achieving High Resolution Ion Mobility Separations Using Traveling Waves in Compact Multiturn Structures for Lossless Ion Manipulations

    SciTech Connect

    Hamid, Ahmed M.; Garimella, Sandilya V. B.; Ibrahim, Yehia M.; Deng, Liulin; Zheng, Xueyun; Webb, Ian K.; Anderson, Gordon A.; Prost, Spencer A.; Norheim, Randolph V.; Tolmachev, Aleksey V.; Baker, Erin S.; Smith, Richard D.

    2016-09-20

    We report on ion mobility separations (IMS) achievable using traveling waves in a Structures for Lossless Ion Manipulations (TW-SLIM) module having a 44-cm path length and sixteen 90º turns. The performance of the TW-SLIM module was evaluated for ion transmission, and ion mobility separations with different RF, TW parameters and SLIM surface gaps in conjunction with mass spectrometry. In this work TWs were created by the transient and dynamic application of DC potentials. The TW-SLIM module demonstrated highly robust performance and the ion mobility resolution achieved even with sixteen close spaced turns was comparable to a similar straight path TW-SLIM module. We found an ion mobility peak capacity of ~ 31 and peak generation rate of 780 s-1 for TW speeds of <210 m/s using the current multi-turn TW-SLIM module. The separations achieved for isomers of peptides and tetrasaccharides were found to be comparable to those from a ~ 0.9-m drift tube-based IMS-MS platform operated at the same pressure (4 torr). The combined attributes of flexible design, low voltage requirements and lossless ion transmission through multiple turns for the present TW-SLIM module provides a basis for SLIM devices capable of achieving much greater ion mobility resolutions via greatly extended ion path lengths and compact serpentine designs that do not significantly impact the instrumentation profile, a direction described in a companion manuscript.

  3. The design of an animal PET: flexible geometry for achieving optimal spatial resolution or high sensitivity.

    PubMed

    Weber, S; Terstegge, A; Herzog, H; Reinartz, R; Reinhart, P; Rongen, F; Müller-Gärtner, H W; Halling, H

    1997-10-01

    We present the design of a positron emission tomograph (PET) with flexible geometry dedicated to in vivo studies of small animals (TierPET). The scanner uses two pairs of detectors. Each detector consists of 400 small individual yttrium aluminum perovskite (YAP) scintillator crystals of dimensions 2 x 2 x 15 mm3, optically isolated and glued together, which are coupled to position-sensitive photomultiplier tubes (PSPMT's). The detector modules can be moved in a radial direction so that the detector-to-detector spacing can be varied. Special hardware has been built for coincidence detection, position detection, and real-time data acquisition, which is performed by a PC. The single-event data are transferred to workstations where the radioactivity distribution is reconstructed. The dimensions of the crystals and the detector layout are the result of extensive simulations which are described in this report, taking into account sensitivity, spatial resolution and additional parameters like parallax error or scatter effects. For the three-dimensional (3-D) reconstruction a genuine 3-D expectation-maximization (EM)-algorithm which can include the characteristics of the detector system has been implemented. The reconstruction software is flexible and matches the different detector configurations. The main advantage of the proposed animal PET scanner is its high flexibility, allowing the realization of various detector-system configurations. By changing the detector-to-detector spacing, the system is capable of either providing good spatial resolution or high sensitivity for dynamic studies of pharmacokinetics.

  4. Achieving high-resolution in flat-panel imagers for digital radiography

    NASA Astrophysics Data System (ADS)

    Rahn, Jeffrey T.; Lemmi, Francesco; Lu, Jeng-Ping; Mei, Ping; Street, Robert A.; Ready, Steve E.; Ho, Jackson; Apte, Raj B.; Van Schuylenbergh, Koenraad; Lau, Rachel; Weisfield, Richard L.; Lujan, Rene; Boyce, James B.

    1999-10-01

    Amorphous silicon (a-Si:H) matrix-addressed imager sensors are the leading new technology for digital medical x-ray imaging. Large-area systems are now commercially available with good resolution and large dynamic range. These systems image x-rays either by detecting light emission from a phosphor screen onto an a-Si:H photodiode, or by collecting ionization charge in a thick x-ray absorbing photoconductor with as selenium, and both approaches have been widely discussed in the literature. While these systems meet the performance needs for general radiographic imaging, further improvements in sensitivity, noise and resolution are needed to fully satisfy the requirements for fluoroscopy and mammography. The approach taken for this paper uses indirect detection, with a phosphor layer for x-ray conversion. The thin a-Si:H photodiode layer for detects the scintillation light. In contrast with the present generation of devices, which have a mesa-isolated sensor at each pixel, these imagers use a continuous sensor covering the entire front surface of the array. The p+ and i layers of a-Si:H are continuous, while the n+ contact has been patterned to isolate adjacent pixels. The continuous photodiode layer maximizes light absorption from the phosphor and provides high x-ray conversion efficiency.

  5. Apparatus and method to achieve high-resolution microscopy with non-diffracting or refracting radiation

    DOEpatents

    Tobin, Jr., Kenneth W.; Bingham, Philip R.; Hawari, Ayman I.

    2012-11-06

    An imaging system employing a coded aperture mask having multiple pinholes is provided. The coded aperture mask is placed at a radiation source to pass the radiation through. The radiation impinges on, and passes through an object, which alters the radiation by absorption and/or scattering. Upon passing through the object, the radiation is detected at a detector plane to form an encoded image, which includes information on the absorption and/or scattering caused by the material and structural attributes of the object. The encoded image is decoded to provide a reconstructed image of the object. Because the coded aperture mask includes multiple pinholes, the radiation intensity is greater than a comparable system employing a single pinhole, thereby enabling a higher resolution. Further, the decoding of the encoded image can be performed to generate multiple images of the object at different distances from the detector plane. Methods and programs for operating the imaging system are also disclosed.

  6. Focal plane actuation to achieve ultra-high resolution on suborbital balloon payloads

    NASA Astrophysics Data System (ADS)

    Scowen, Paul A.; Miller, Alex; Challa, Priya; Veach, Todd; Groppi, Chris; Mauskopf, Phil

    2014-07-01

    Over the past few years there has been remarkable success flying imaging telescope systems suspended from suborbital balloon payload systems. These imaging systems have covered optical, ultraviolet, sub-­-millimeter and infrared passbands (i.e. BLAST, STO, SBI, Fireball and others). In recognition of these advances NASA is now considering ambitious programs to promote planetary imaging from high altitude at a fraction of the cost of similar fully orbital systems. The challenge with imaging from a balloon payload is delivering the full diffraction-­-limited resolution of the system from a moving payload. Good progress has been made with damping mechanisms and oscillation control to remove most macroscopic movement in the departures of the imaging focal plane from a static configuration, however a jitter component remains that is difficult to remove using external corrections. This paper reports on work to demonstrate in the laboratory the utility and performance of actuating a detector focal plane (of whatever type) to remove the final jitter terms using an agile hexapod design. The input to this demonstration is the jitter signal generated by the pointing system of a previously flown balloon mission (the Stratospheric Terahertz Observatory, STO). Our group has a mature jitter compensation system that thermally isolates the control head from the focal plane itself. This allows the hexapod to remain at ambient temperature in a vacuum environment with the focal plane cooled to cryogenic temperatures. Our lab design mounts the focal plane on the hexapod in a custom cryostat and delivers an active optical stimulus together with the corresponding jitter signal, using the actuation of the hexapod to correct for the departures from a static, stable configuration. We believe this demonstration will make the case for inclusion of this technological solution in future balloon-­-borne imaging systems requiring ultra-­-high resolution.

  7. Resolution limits of ultrafast ultrasound localization microscopy

    NASA Astrophysics Data System (ADS)

    Desailly, Yann; Pierre, Juliette; Couture, Olivier; Tanter, Mickael

    2015-11-01

    As in other imaging methods based on waves, the resolution of ultrasound imaging is limited by the wavelength. However, the diffraction-limit can be overcome by super-localizing single events from isolated sources. In recent years, we developed plane-wave ultrasound allowing frame rates up to 20 000 fps. Ultrafast processes such as rapid movement or disruption of ultrasound contrast agents (UCA) can thus be monitored, providing us with distinct punctual sources that could be localized beyond the diffraction limit. We previously showed experimentally that resolutions beyond λ/10 can be reached in ultrafast ultrasound localization microscopy (uULM) using a 128 transducer matrix in reception. Higher resolutions are theoretically achievable and the aim of this study is to predict the maximum resolution in uULM with respect to acquisition parameters (frequency, transducer geometry, sampling electronics). The accuracy of uULM is the error on the localization of a bubble, considered a point-source in a homogeneous medium. The proposed model consists in two steps: determining the timing accuracy of the microbubble echo in radiofrequency data, then transferring this time accuracy into spatial accuracy. The simplified model predicts a maximum resolution of 40 μm for a 1.75 MHz transducer matrix composed of two rows of 64 elements. Experimental confirmation of the model was performed by flowing microbubbles within a 60 μm microfluidic channel and localizing their blinking under ultrafast imaging (500 Hz frame rate). The experimental resolution, determined as the standard deviation in the positioning of the microbubbles, was predicted within 6 μm (13%) of the theoretical values and followed the analytical relationship with respect to the number of elements and depth. Understanding the underlying physical principles determining the resolution of superlocalization will allow the optimization of the imaging setup for each organ. Ultimately, accuracies better than the size

  8. Interpreting 16S metagenomic data without clustering to achieve sub-OTU resolution

    PubMed Central

    Tikhonov, Mikhail; Leach, Robert W; Wingreen, Ned S

    2015-01-01

    The standard approach to analyzing 16S tag sequence data, which relies on clustering reads by sequence similarity into Operational Taxonomic Units (OTUs), underexploits the accuracy of modern sequencing technology. We present a clustering-free approach to multi-sample Illumina data sets that can identify independent bacterial subpopulations regardless of the similarity of their 16S tag sequences. Using published data from a longitudinal time-series study of human tongue microbiota, we are able to resolve within standard 97% similarity OTUs up to 20 distinct subpopulations, all ecologically distinct but with 16S tags differing by as little as one nucleotide (99.2% similarity). A comparative analysis of oral communities of two cohabiting individuals reveals that most such subpopulations are shared between the two communities at 100% sequence identity, and that dynamical similarity between subpopulations in one host is strongly predictive of dynamical similarity between the same subpopulations in the other host. Our method can also be applied to samples collected in cross-sectional studies and can be used with the 454 sequencing platform. We discuss how the sub-OTU resolution of our approach can provide new insight into factors shaping community assembly. PMID:25012900

  9. Reaching the Diffraction Limit - Differential Speckle and Wide-Field Imaging for the Gemini-N Telescope

    NASA Technical Reports Server (NTRS)

    Scott, Nic J.; Howell, Steve; Horch, Elliott

    2016-01-01

    Speckle imaging allows telescopes to achieve di raction limited imaging performance. The technique requires cameras capable of reading out frames at a very fast rate, e ectively `freezing out' atmospheric seeing. The resulting speckles can be correlated and images reconstructed that are at the di raction limit of the telescope. These new instruments are based on the successful performance and design of the Di erential Speckle Survey Instrument (DSSI) [2, 1]. The instruments are being built for the Gemini-N and WIYN telescopes and will be made available to the community via the peer review proposal process. We envision their primary use to be validation and characterization of exoplanet targets from the NASA K2 and TESS missions and RV discovered exoplanets. Such targets will provide excellent follow-up candidates for both the WIYN and Gemini telescopes [3]. Examples of DSSI data are shown in the gures below. We expect similar data quality in speckle imaging mode with the new instruments. Additionally, both cameras will have a wide- eld mode and standard SDSS lters. They will be highly versatile instruments and it is that likely many other science programs will request time on the cameras. The limiting magnitude for speckle observations, will remain around 13-14th at WIYN and 16-17th at Gemini, while wide- eld, normal CCD imaging operation should be able to go to much fainter, providing usual CCD imaging and photometric capabilities. The instruments will also have high utility as scoring cameras for telescope engineering purposes, or other applications where high time resolution is needed. Instrument support will be provided, including a software pipeline that takes raw speckle data to fully reconstructed images.

  10. Subsurface Super-resolution Imaging of Unstained Polymer Nanostructures

    NASA Astrophysics Data System (ADS)

    Urban, Ben E.; Dong, Biqin; Nguyen, The-Quyen; Backman, Vadim; Sun, Cheng; Zhang, Hao F.

    2016-06-01

    Optical imaging has offered unique advantages in material researches, such as spectroscopy and lifetime measurements of deeply embedded materials, which cannot be matched using electron or scanning-probe microscopy. Unfortunately, conventional optical imaging cannot provide the spatial resolutions necessary for many nanoscopic studies. Despite recent rapid progress, super-resolution optical imaging has yet to be widely applied to non-biological materials. Herein we describe a method for nanoscopic optical imaging of buried polymer nanostructures without the need for extrinsic staining. We observed intrinsic stochastic fluorescence emission or blinking from unstained polymers and performed spatial-temporal spectral analysis to investigate its origin. We further applied photon localization super-resolution imaging reconstruction to the detected stochastic blinking, and achieved a spatial resolution of at least 100 nm, which corresponds to a six-fold increase over the optical diffraction limit. This work demonstrates the potential for studying the static heterogeneities of intrinsic polymer molecular-specific properties at sub-diffraction-limited optical resolutions.

  11. Subsurface Super-resolution Imaging of Unstained Polymer Nanostructures

    PubMed Central

    Urban, Ben E.; Dong, Biqin; Nguyen, The-Quyen; Backman, Vadim; Sun, Cheng; Zhang, Hao F.

    2016-01-01

    Optical imaging has offered unique advantages in material researches, such as spectroscopy and lifetime measurements of deeply embedded materials, which cannot be matched using electron or scanning-probe microscopy. Unfortunately, conventional optical imaging cannot provide the spatial resolutions necessary for many nanoscopic studies. Despite recent rapid progress, super-resolution optical imaging has yet to be widely applied to non-biological materials. Herein we describe a method for nanoscopic optical imaging of buried polymer nanostructures without the need for extrinsic staining. We observed intrinsic stochastic fluorescence emission or blinking from unstained polymers and performed spatial-temporal spectral analysis to investigate its origin. We further applied photon localization super-resolution imaging reconstruction to the detected stochastic blinking, and achieved a spatial resolution of at least 100 nm, which corresponds to a six-fold increase over the optical diffraction limit. This work demonstrates the potential for studying the static heterogeneities of intrinsic polymer molecular-specific properties at sub-diffraction-limited optical resolutions. PMID:27354178

  12. Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI).

    PubMed

    Dertinger, T; Colyer, R; Iyer, G; Weiss, S; Enderlein, J

    2009-12-29

    Super-resolution optical microscopy is a rapidly evolving area of fluorescence microscopy with a tremendous potential for impacting many fields of science. Several super-resolution methods have been developed over the last decade, all capable of overcoming the fundamental diffraction limit of light. We present here an approach for obtaining subdiffraction limit optical resolution in all three dimensions. This method relies on higher-order statistical analysis of temporal fluctuations (caused by fluorescence blinking/intermittency) recorded in a sequence of images (movie). We demonstrate a 5-fold improvement in spatial resolution by using a conventional wide-field microscope. This resolution enhancement is achieved in iterative discrete steps, which in turn allows the evaluation of images at different resolution levels. Even at the lowest level of resolution enhancement, our method features significant background reduction and thus contrast enhancement and is demonstrated on quantum dot-labeled microtubules of fibroblast cells.

  13. Super-Resolution Scanning Laser Microscopy Based on Virtually Structured Detection

    PubMed Central

    Zhi, Yanan; Wang, Benquan; Yao, Xincheng

    2016-01-01

    Light microscopy plays a key role in biological studies and medical diagnosis. The spatial resolution of conventional optical microscopes is limited to approximately half the wavelength of the illumination light as a result of the diffraction limit. Several approaches—including confocal microscopy, stimulated emission depletion microscopy, stochastic optical reconstruction microscopy, photoactivated localization microscopy, and structured illumination microscopy—have been established to achieve super-resolution imaging. However, none of these methods is suitable for the super-resolution ophthalmoscopy of retinal structures because of laser safety issues and inevitable eye movements. We recently experimentally validated virtually structured detection (VSD) as an alternative strategy to extend the diffraction limit. Without the complexity of structured illumination, VSD provides an easy, low-cost, and phase artifact–free strategy to achieve super-resolution in scanning laser microscopy. In this article we summarize the basic principles of the VSD method, review our demonstrated single-point and line-scan super-resolution systems, and discuss both technical challenges and the potential of VSD-based instrumentation for super-resolution ophthalmoscopy of the retina. PMID:27480461

  14. Super-Resolution Scanning Laser Microscopy Based on Virtually Structured Detection.

    PubMed

    Zhi, Yanan; Wang, Benquan; Yao, Xincheng

    2015-01-01

    Light microscopy plays a key role in biological studies and medical diagnosis. The spatial resolution of conventional optical microscopes is limited to approximately half the wavelength of the illumination light as a result of the diffraction limit. Several approaches-including confocal microscopy, stimulated emission depletion microscopy, stochastic optical reconstruction microscopy, photoactivated localization microscopy, and structured illumination microscopy-have been established to achieve super-resolution imaging. However, none of these methods is suitable for the super-resolution ophthalmoscopy of retinal structures because of laser safety issues and inevitable eye movements. We recently experimentally validated virtually structured detection (VSD) as an alternative strategy to extend the diffraction limit. Without the complexity of structured illumination, VSD provides an easy, low-cost, and phase artifact-free strategy to achieve super-resolution in scanning laser microscopy. In this article we summarize the basic principles of the VSD method, review our demonstrated single-point and line-scan super-resolution systems, and discuss both technical challenges and the potential of VSD-based instrumentation for super-resolution ophthalmoscopy of the retina.

  15. DBR tapered diode laser with 12.7 W output power and nearly diffraction-limited, narrowband emission at 1030 nm

    NASA Astrophysics Data System (ADS)

    Müller, André; Fricke, Jörg; Bugge, Frank; Brox, Olaf; Erbert, Götz; Sumpf, Bernd

    2016-04-01

    A 1030 nm distributed Bragg reflector (DBR) tapered diode laser with nearly diffraction-limited emission is presented. The laser provides an optical output power of 12.7 W with an electro-optical efficiency >40 %. At 10.5 W of optical output power, a central lobe power content of 8.1 W and a nearly diffraction-limited beam propagation ratio of M 2 = 1.1 (1/ e 2) are obtained. The corresponding brightness is 700 MW cm-2 sr-1. Compared to previous approaches, intrinsic wavelength stabilization is obtained by a third-order DBR grating manufactured using more reproducible electron-beam lithography. A narrowband emission is measured over the whole power range with a spectral bandwidth of about 17 pm at 12.5 W. Based on the measured electro-optical, spectral and spatial properties, the laser is suitable for applications requiring narrowband, high-power emission with high spatial quality.

  16. Axial super-resolution evanescent wave tomography

    NASA Astrophysics Data System (ADS)

    Pendharker, Sarang; Shende, Swapnali; Newman, Ward; Ogg, Stephen; Nazemifard, Neda; Jacob, Zubin

    2016-12-01

    Optical tomographic reconstruction of a 3D nanoscale specimen is hindered by the axial diffraction limit, which is 2-3 times worse than the focal plane resolution. We propose and experimentally demonstrate an axial super-resolution evanescent wave tomography (AxSET) method that enables the use of regular evanescent wave microscopes like Total Internal Reflection Fluorescence Microscope (TIRF) beyond surface imaging, and achieve tomographic reconstruction with axial super-resolution. Our proposed method based on Fourier reconstruction achieves axial super-resolution by extracting information from multiple sets of three-dimensional fluorescence images when the sample is illuminated by an evanescent wave. We propose a procedure to extract super-resolution features from the incremental penetration of an evanescent wave and support our theory by 1D (along the optical axis) and 3D simulations. We validate our claims by experimentally demonstrating tomographic reconstruction of microtubules in HeLa cells with an axial resolution of $\\sim$130 nm. Our method does not require any additional optical components or sample preparation. The proposed method can be combined with focal plane super-resolution techniques like STORM and can also be adapted for THz and microwave near-field tomography.

  17. Axial super-resolution evanescent wave tomography.

    PubMed

    Pendharker, Sarang; Shende, Swapnali; Newman, Ward; Ogg, Stephen; Nazemifard, Neda; Jacob, Zubin

    2016-12-01

    Optical tomographic reconstruction of a three-dimensional (3D) nanoscale specimen is hindered by the axial diffraction limit, which is 2-3 times worse than the focal plane resolution. We propose and experimentally demonstrate an axial super-resolution evanescent wave tomography method that enables the use of regular evanescent wave microscopes like the total internal reflection fluorescence microscope beyond surface imaging and achieve a tomographic reconstruction with axial super-resolution. Our proposed method based on Fourier reconstruction achieves axial super-resolution by extracting information from multiple sets of 3D fluorescence images when the sample is illuminated by an evanescent wave. We propose a procedure to extract super-resolution features from the incremental penetration of an evanescent wave and support our theory by one-dimensional (along the optical axis) and 3D simulations. We validate our claims by experimentally demonstrating tomographic reconstruction of microtubules in HeLa cells with an axial resolution of ∼130  nm. Our method does not require any additional optical components or sample preparation. The proposed method can be combined with focal plane super-resolution techniques like stochastic optical reconstruction microscopy and can also be adapted for THz and microwave near-field tomography.

  18. Super-resolution photoacoustic microscopy using photonic nanojets: a simulation study.

    PubMed

    Upputuri, Paul Kumar; Wen, Zhuo-Bin; Wu, Zhe; Pramanik, Manojit

    2014-01-01

    Optical resolution photoacoustic microscopy (ORPAM) is important for various biomedical applications, such as the study of cellular structures, microcirculation systems, and tumor angiogenesis. However, the lateral resolution of a conventional ORPAM is limited by optical diffraction. In this work, we report a simulation study to achieve subdiffraction-limited super-resolution in ORPAM using microspheres. Laser radiation is focused through a microsphere to generate a photonic nanojet, which provides the possibility to break the diffraction limit in ORPAM by reducing the size of the excitation volume. In our simulations using microspheres, we observed improvement in the lateral resolution up to compared to conventional ORPAM. The method is simple, cost effective, and can provide far-field resolution. This approach may provide new opportunities for many biomedical imaging applications that require finer resolution.

  19. Super-resolution photoacoustic microscopy using photonic nanojets: a simulation study

    NASA Astrophysics Data System (ADS)

    Upputuri, Paul Kumar; Wen, Zhuo-Bin; Wu, Zhe; Pramanik, Manojit

    2014-11-01

    Optical resolution photoacoustic microscopy (ORPAM) is important for various biomedical applications, such as the study of cellular structures, microcirculation systems, and tumor angiogenesis. However, the lateral resolution of a conventional ORPAM is limited by optical diffraction. In this work, we report a simulation study to achieve subdiffraction-limited super-resolution in ORPAM using microspheres. Laser radiation is focused through a microsphere to generate a photonic nanojet, which provides the possibility to break the diffraction limit in ORPAM by reducing the size of the excitation volume. In our simulations using microspheres, we observed improvement in the lateral resolution up to ˜fourfold compared to conventional ORPAM. The method is simple, cost effective, and can provide far-field resolution. This approach may provide new opportunities for many biomedical imaging applications that require finer resolution.

  20. Mirror-enhanced super-resolution microscopy.

    PubMed

    Yang, Xusan; Xie, Hao; Alonas, Eric; Liu, Yujia; Chen, Xuanze; Santangelo, Philip J; Ren, Qiushi; Xi, Peng; Jin, Dayong

    Axial excitation confinement beyond the diffraction limit is crucial to the development of next-generation, super-resolution microscopy. STimulated Emission Depletion (STED) nanoscopy offers lateral super-resolution using a donut-beam depletion, but its axial resolution is still over 500 nm. Total internal reflection fluorescence microscopy is widely used for single-molecule localization, but its ability to detect molecules is limited to within the evanescent field of ~ 100 nm from the cell attachment surface. We find here that the axial thickness of the point spread function (PSF) during confocal excitation can be easily improved to 110 nm by replacing the microscopy slide with a mirror. The interference of the local electromagnetic field confined the confocal PSF to a 110-nm spot axially, which enables axial super-resolution with all laser-scanning microscopes. Axial sectioning can be obtained with wavelength modulation or by controlling the spacer between the mirror and the specimen. With no additional complexity, the mirror-assisted excitation confinement enhanced the axial resolution six-fold and the lateral resolution two-fold for STED, which together achieved 19-nm resolution to resolve the inner rim of a nuclear pore complex and to discriminate the contents of 120 nm viral filaments. The ability to increase the lateral resolution and decrease the thickness of an axial section using mirror-enhanced STED without increasing the laser power is of great importance for imaging biological specimens, which cannot tolerate high laser power.

  1. Mirror-enhanced super-resolution microscopy

    PubMed Central

    Yang, Xusan; Xie, Hao; Alonas, Eric; Liu, Yujia; Chen, Xuanze; Santangelo, Philip J; Ren, Qiushi; Xi, Peng; Jin, Dayong

    2016-01-01

    Axial excitation confinement beyond the diffraction limit is crucial to the development of next-generation, super-resolution microscopy. STimulated Emission Depletion (STED) nanoscopy offers lateral super-resolution using a donut-beam depletion, but its axial resolution is still over 500 nm. Total internal reflection fluorescence microscopy is widely used for single-molecule localization, but its ability to detect molecules is limited to within the evanescent field of ~ 100 nm from the cell attachment surface. We find here that the axial thickness of the point spread function (PSF) during confocal excitation can be easily improved to 110 nm by replacing the microscopy slide with a mirror. The interference of the local electromagnetic field confined the confocal PSF to a 110-nm spot axially, which enables axial super-resolution with all laser-scanning microscopes. Axial sectioning can be obtained with wavelength modulation or by controlling the spacer between the mirror and the specimen. With no additional complexity, the mirror-assisted excitation confinement enhanced the axial resolution six-fold and the lateral resolution two-fold for STED, which together achieved 19-nm resolution to resolve the inner rim of a nuclear pore complex and to discriminate the contents of 120 nm viral filaments. The ability to increase the lateral resolution and decrease the thickness of an axial section using mirror-enhanced STED without increasing the laser power is of great importance for imaging biological specimens, which cannot tolerate high laser power. PMID:27398242

  2. Super-resolution nanofabrication with metal-ion doped hybrid material through an optical dual-beam approach

    SciTech Connect

    Cao, Yaoyu; Li, Xiangping; Gu, Min

    2014-12-29

    We apply an optical dual-beam approach to a metal-ion doped hybrid material to achieve nanofeatures beyond the optical diffraction limit. By spatially inhibiting the photoreduction and the photopolymerization, we realize a nano-line, consisting of polymer matrix and in-situ generated gold nanoparticles, with a lateral size of sub 100 nm, corresponding to a factor of 7 improvement compared to the diffraction limit. With the existence of gold nanoparticles, a plasmon enhanced super-resolution fabrication mechanism in the hybrid material is observed, which benefits in a further reduction in size of the fabricated feature. The demonstrated nanofeature in hybrid materials paves the way for realizing functional nanostructures.

  3. Analytical description of high-aperture STED resolution with 0–2π vortex phase modulation

    PubMed Central

    Xie, Hao; Liu, Yujia; Jin, Dayong; Santangelo, Philip J.; Xi, Peng

    2014-01-01

    Stimulated emission depletion (STED) can achieve optical superresolution, with the optical diffraction limit broken by the suppression on the periphery of the fluorescent focal spot. Previously, it is generally experimentally accepted that there exists an inverse square root relationship with the STED power and the resolution, but with arbitrary coefficients in expression. In this paper, we have removed the arbitrary coefficients by exploring the relationship between the STED power and the achievable resolution from vector optical theory for the widely used 0–2π vortex phase modulation. Electromagnetic fields of the focal region of a high numerical aperture objective are calculated and approximated into polynomials of radius in the focal plane, and analytical expression of resolution as a function of the STED intensity has been derived. As a result, the resolution can be estimated directly from the measurement of the saturation power of the dye and the STED power applied in the region of high STED power. PMID:24323224

  4. Super-resolution two-photon microscopy via scanning patterned illumination

    NASA Astrophysics Data System (ADS)

    Urban, Ben E.; Yi, Ji; Chen, Siyu; Dong, Biqin; Zhu, Yongling; DeVries, Steven H.; Backman, Vadim; Zhang, Hao F.

    2015-04-01

    We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microscopy. Using 2P-SPIM we achieved a lateral resolution of 141 nm, which represents an improvement by a factor of 1.9 over the corresponding diffraction limit. We further demonstrated super-resolution cellular imaging by 2P-SPIM to image actin cytoskeleton in mammalian cells and three-dimensional imaging in highly scattering retinal tissue.

  5. Rapid super-resolution line-scanning microscopy through virtually structured detection.

    PubMed

    Zhi, Yanan; Lu, Rongwen; Wang, Benquan; Zhang, Qiuxiang; Yao, Xincheng

    2015-04-15

    Virtually structured detection (VSD) has been demonstrated to break the diffraction limit in scanning laser microscopy (SLM). VSD provides an easy, low-cost, and phase-artifact-free strategy to achieve super-resolution imaging. However, practical application of this method is challenging due to a limited image acquisition speed. We report here the combination of VSD and line-scanning microscopy (LSM) to improve the image acquisition speed. A motorized dove prism was used to achieve automatic control of four-angle (i.e., 0°, 45°, 90°, and 135°) scanning, thus ensuring isotropic resolution improvement. Both an optical resolution target and a living frog eyecup were used to verify resolution enhancement.

  6. Comparative and practical aspects of localization-based super-resolution imaging.

    PubMed

    Laevsky, Gary S; O'Connell, Christopher B

    2013-01-01

    Super-resolution microscopy overcomes diffraction to generate images with superior resolution compared to conventional light microscopy. Localization-based super-resolution methods result in up to ten-fold improvement in resolution by determining the positions of fluorescent molecules with sub-pixel accuracy. This process critically depends on controlled emission at the level of individual fluorophores so that fluorescence is non-overlapping, allowing for accurate centroid determination of diffraction-limited spots by Gaussian fitting of the pixel intensities. The intrinsic photoswitching behavior of many fluorophores provides a convenient way to achieve emitter isolation. Here, we describe methods for label preparation and staining of cellular structures to obtain high-quality images using localization super resolution. We also compare labeling strategies and dye characteristics relevant to all localization-based techniques, such as STORM and PALM.

  7. Multi-pulse pumping for far-field super-resolution imaging

    NASA Astrophysics Data System (ADS)

    Requena, Sebastian; Raut, Sangram; Doan, Hung; Kimball, Joe; Fudala, Rafal; Borejdo, Julian; Gryczynski, Ignacy; Strzhemechny, Yuri; Gryczynski, Zygmunt

    2016-02-01

    Recently, far-field optical imaging with a resolution significantly beyond diffraction limit has attracted tremendous attention allowing for high resolution imaging in living objects. Various methods have been proposed that are divided in to two basic approaches; deterministic super-resolution like STED or RESOLFT and stochastic super-resolution like PALM or STORM. We propose to achieve super-resolution in far-field fluorescence imaging by the use of controllable (on-demand) bursts of pulses that can change the fluorescence signal of long-lived component over one order of magnitude. We demonstrate that two beads, one labeled with a long-lived dye and another with a short-lived dye, separated by a distance lower than 100 nm can be easily resolved in a single experiment. The proposed method can be used to separate two biological structures in a cell by targeting them with two antibodies labeled with long-lived and short-lived fluorophores.

  8. Using a short-pulse diffraction-limited laser beam to probe filamentation of a random phase plate smoothed beam.

    PubMed

    Kline, J L; Montgomery, D S; Flippo, K A; Johnson, R P; Rose, H A; Shimada, T; Williams, E A

    2008-10-01

    A short pulse (few picoseconds) laser probe provides high temporal resolution measurements to elucidate details of fast dynamic phenomena not observable with typical longer laser pulse probes and gated diagnostics. Such a short pulse laser probe (SPLP) has been used to measure filamentation of a random phase plate (RPP) smoothed laser beam in a gas-jet plasma. The plasma index of refraction due to driven density and temperature fluctuations by the RPP beam perturbs the phase front of a SPLP propagating at a 90 degree angle with respect to the RPP interaction beam. The density and temperature fluctuations are quasistatic on the time scale of the SPLP (approximately 2 ps). The transmitted near-field intensity distribution from the SPLP provides a measure of the phase front perturbation. At low plasma densities, the transmitted intensity pattern is asymmetric with striations across the entire probe beam in the direction of the RPP smoothed beam. As the plasma density increases, the striations break up into smaller sizes along the direction of the RPP beam propagation. The breakup of the intensity pattern is consistent with self-focusing of the RPP smoothed interaction beam. Simulations of the experiment using the wave propagation code, PF3D, are in qualitative agreement demonstrating that the asymmetric striations can be attributed to the RPP driven density fluctuations. Quantification of the beam breakup measured by the transmitted SPLP could lead to a new method for measuring self-focusing of lasers in underdense plasmas.

  9. High resolution telescope including an array of elemental telescopes aligned along a common axis and supported on a space frame with a pivot at its geometric center

    DOEpatents

    Norbert, M.A.; Yale, O.

    1992-04-28

    A large effective-aperture, low-cost optical telescope with diffraction-limited resolution enables ground-based observation of near-earth space objects. The telescope has a non-redundant, thinned-aperture array in a center-mount, single-structure space frame. It employes speckle interferometric imaging to achieve diffraction-limited resolution. The signal-to-noise ratio problem is mitigated by moving the wavelength of operation to the near-IR, and the image is sensed by a Silicon CCD. The steerable, single-structure array presents a constant pupil. The center-mount, radar-like mount enables low-earth orbit space objects to be tracked as well as increases stiffness of the space frame. In the preferred embodiment, the array has elemental telescopes with subaperture of 2.1 m in a circle-of-nine configuration. The telescope array has an effective aperture of 12 m which provides a diffraction-limited resolution of 0.02 arc seconds. Pathlength matching of the telescope array is maintained by a electro-optical system employing laser metrology. Speckle imaging relaxes pathlength matching tolerance by one order of magnitude as compared to phased arrays. Many features of the telescope contribute to substantial reduction in costs. These include eliminating the conventional protective dome and reducing on-site construction activities. The cost of the telescope scales with the first power of the aperture rather than its third power as in conventional telescopes. 15 figs.

  10. High resolution telescope including an array of elemental telescopes aligned along a common axis and supported on a space frame with a pivot at its geometric center

    DOEpatents

    Norbert, Massie A.; Yale, Oster

    1992-01-01

    A large effective-aperture, low-cost optical telescope with diffraction-limited resolution enables ground-based observation of near-earth space objects. The telescope has a non-redundant, thinned-aperture array in a center-mount, single-structure space frame. It employes speckle interferometric imaging to achieve diffraction-limited resolution. The signal-to-noise ratio problem is mitigated by moving the wavelength of operation to the near-IR, and the image is sensed by a Silicon CCD. The steerable, single-structure array presents a constant pupil. The center-mount, radar-like mount enables low-earth orbit space objects to be tracked as well as increases stiffness of the space frame. In the preferred embodiment, the array has elemental telescopes with subaperture of 2.1 m in a circle-of-nine configuration. The telescope array has an effective aperture of 12 m which provides a diffraction-limited resolution of 0.02 arc seconds. Pathlength matching of the telescope array is maintained by a electro-optical system employing laser metrology. Speckle imaging relaxes pathlength matching tolerance by one order of magnitude as compared to phased arrays. Many features of the telescope contribute to substantial reduction in costs. These include eliminating the conventional protective dome and reducing on-site construction activities. The cost of the telescope scales with the first power of the aperture rather than its third power as in conventional telescopes.

  11. Going far beyond the near-field diffraction limit via plasmonic cavity lens with high spatial frequency spectrum off-axis illumination

    NASA Astrophysics Data System (ADS)

    Zhao, Zeyu; Luo, Yunfei; Zhang, Wei; Wang, Changtao; Gao, Ping; Wang, Yanqin; Pu, Mingbo; Yao, Na; Zhao, Chengwei; Luo, Xiangang

    2015-10-01

    For near-field imaging optics, minimum resolvable feature size is highly constrained by the near-field diffraction limit associated with the illumination light wavelength and the air distance between the imaging devices and objects. In this study, a plasmonic cavity lens composed of Ag-photoresist-Ag form incorporating high spatial frequency spectrum off-axis illumination (OAI) is proposed to realize deep subwavelength imaging far beyond the near-field diffraction limit. This approach benefits from the resonance effect of the plasmonic cavity lens and the wavevector shifting behavior via OAI, which remarkably enhances the object’s subwavelength information and damps negative imaging contribution from the longitudinal electric field component in imaging region. Experimental images of well resolved 60-nm half-pitch patterns under 365-nm ultra-violet light are demonstrated at air distance of 80 nm between the mask patterns and plasmonic cavity lens, approximately four-fold longer than that in the conventional near-field lithography and superlens scheme. The ultimate air distance for the 60-nm half-pitch object could be theoretically extended to 120 nm. Moreover, two-dimensional L-shape patterns and deep subwavelength patterns are illustrated via simulations and experiments. This study promises the significant potential to make plasmonic lithography as a practical, cost-effective, simple and parallel nano-fabrication approach.

  12. Super-resolution with Toraldo pupils: analysis with electromagnetic numerical simulations

    NASA Astrophysics Data System (ADS)

    Olmi, Luca; Bolli, Pietro; Cresci, Luca; Mugnai, Daniela; Natale, Enzo; Nesti, Renzo; Panella, Dario; Stefani, Lorenzo

    2016-07-01

    The concept of super-resolution refers to various methods for improving the angular resolution of an optical imaging system beyond the classical diffraction limit. In optical microscopy, several techniques have been developed with the aim of narrowing the central lobe of the illumination Point Spread Function (PSF). In Astronomy a few methods have been proposed to achieve reflector telescopes and antennas with resolution significantly better than the diffraction limit but, to our best knowledge, no working system is in operation. A possible practical approach consists of using the so-called "Toraldo Pupils" (TPs) or variable transmittance filters. These pupils were introduced by G. Toraldo di Francia in 1952,1 and consist of a series of discrete, concentric circular coronae providing specific optical transparency and dephasing in order to engineer the required PSF. The first successful laboratory test of TPs in the microwaves was achieved in 2003,2 and in the present work we build upon these initial measurements to perform electromagnetic (EM) numerical simulations of TPs, using a commercial full-wave software tool. These simulations were used to study various EM effects that can mask and/or affect the performance of the pupils and to analyze the near-field as well as the far-field response. Our EM analysis confirms that at 20 GHz the width of the central lobe in the far-field generated by a TP significantly decreases compared to a clear circular aperture with the same diameter.

  13. Super-resolution reconstruction algorithm based on adaptive convolution kernel size selection

    NASA Astrophysics Data System (ADS)

    Gao, Hang; Chen, Qian; Sui, Xiubao; Zeng, Junjie; Zhao, Yao

    2016-09-01

    Restricted by the detector technology and optical diffraction limit, the spatial resolution of infrared imaging system is difficult to achieve significant improvement. Super-Resolution (SR) reconstruction algorithm is an effective way to solve this problem. Among them, the SR algorithm based on multichannel blind deconvolution (MBD) estimates the convolution kernel only by low resolution observation images, according to the appropriate regularization constraints introduced by a priori assumption, to realize the high resolution image restoration. The algorithm has been shown effective when each channel is prime. In this paper, we use the significant edges to estimate the convolution kernel and introduce an adaptive convolution kernel size selection mechanism, according to the uncertainty of the convolution kernel size in MBD processing. To reduce the interference of noise, we amend the convolution kernel in an iterative process, and finally restore a clear image. Experimental results show that the algorithm can meet the convergence requirement of the convolution kernel estimation.

  14. Super-resolution spinning-disk confocal microscopy using optical photon reassignment.

    PubMed

    Azuma, Takuya; Kei, Takayuki

    2015-06-01

    Spinning-disk confocal microscopy is a proven technology for investigating 3D structures of biological specimens. Here we report a super-resolution method based on spinning-disk confocal microscopy that optically improves lateral resolution by a factor of 1.37 with a single exposure. Moreover, deconvolution yields twofold improvement over the diffraction limit. With the help of newly modified Nipkow disk which comprises pinholes and micro-lenses on the front and back respectively, emitted photons from specimen can be optically reassigned to the most probable locations they originate from. Consequently, the improvement in resolution is achieved preserving inherent sectioning capabilities of confocal microscopy. This extremely simple implementation will enable reliable observations at super high resolution in biomedical routine research.

  15. Nearly diffraction-limited X-ray focusing with variable-numerical-aperture focusing optical system based on four deformable mirrors

    PubMed Central

    Matsuyama, Satoshi; Nakamori, Hiroki; Goto, Takumi; Kimura, Takashi; Khakurel, Krishna P.; Kohmura, Yoshiki; Sano, Yasuhisa; Yabashi, Makina; Ishikawa, Tetsuya; Nishino, Yoshinori; Yamauchi, Kazuto

    2016-01-01

    Unlike the electrostatic and electromagnetic lenses used in electron microscopy, most X-ray focusing optical systems have fixed optical parameters with constant numerical apertures (NAs). This lack of adaptability has significantly limited application targets. In the research described herein, we developed a variable-NA X-ray focusing system based on four deformable mirrors, two sets of Kirkpatrick–Baez-type focusing mirrors, in order to control the focusing size while keeping the position of the focus unchanged. We applied a mirror deformation procedure using optical/X-ray metrology for offline/online adjustments. We performed a focusing test at a SPring-8 beamline and confirmed that the beam size varied from 108 nm to 560 nm (165 nm to 1434 nm) in the horizontal (vertical) direction by controlling the NA while maintaining diffraction-limited conditions. PMID:27097853

  16. High-energy single-longitudinal mode nearly diffraction-limited optical parametric source with 3 MHz frequency stability for CO2 DIAL.

    PubMed

    Raybaut, Myriam; Schmid, Thomas; Godard, Antoine; Mohamed, Ajmal K; Lefebvre, Michel; Marnas, Fabien; Flamant, Pierre; Bohman, Axel; Geiser, Peter; Kaspersen, Peter

    2009-07-01

    We report on a 2.05 microm nanosecond master oscillator power amplifier optical parametric source for CO2 differential-absorption lidar. The master oscillator consists of an entangled-cavity nanosecond optical parametric oscillator based on a type II periodically poled lithium niobate crystal that provides highly stable single-longitudinal-mode radiation. The signal emission is amplified by a multistage parametric amplifier to generate up to 11 mJ in a nearly diffraction-limited beam with an M2 quality factor of approximately 1.5 while maintaining single-longitudinal-mode emission with a frequency stability better than 3 MHz rms. This approach can be readily applied to the detection of various greenhouse gases.

  17. Nearly diffraction-limited X-ray focusing with variable-numerical-aperture focusing optical system based on four deformable mirrors.

    PubMed

    Matsuyama, Satoshi; Nakamori, Hiroki; Goto, Takumi; Kimura, Takashi; Khakurel, Krishna P; Kohmura, Yoshiki; Sano, Yasuhisa; Yabashi, Makina; Ishikawa, Tetsuya; Nishino, Yoshinori; Yamauchi, Kazuto

    2016-04-21

    Unlike the electrostatic and electromagnetic lenses used in electron microscopy, most X-ray focusing optical systems have fixed optical parameters with constant numerical apertures (NAs). This lack of adaptability has significantly limited application targets. In the research described herein, we developed a variable-NA X-ray focusing system based on four deformable mirrors, two sets of Kirkpatrick-Baez-type focusing mirrors, in order to control the focusing size while keeping the position of the focus unchanged. We applied a mirror deformation procedure using optical/X-ray metrology for offline/online adjustments. We performed a focusing test at a SPring-8 beamline and confirmed that the beam size varied from 108 nm to 560 nm (165 nm to 1434 nm) in the horizontal (vertical) direction by controlling the NA while maintaining diffraction-limited conditions.

  18. Methodology for optimal in situ alignment and setting of bendable optics for nearly diffraction-limited focusing of soft x-rays

    NASA Astrophysics Data System (ADS)

    Merthe, Daniel J.; Yashchuk, Valeriy V.; Goldberg, Kenneth A.; Kunz, Martin; Tamura, Nobumichi; McKinney, Wayne R.; Artemiev, Nikolay A.; Celestre, Richard S.; Morrison, Gregory Y.; Anderson, Erik H.; Smith, Brian V.; Domning, Edward E.; Rekawa, Senajith B.; Padmore, Howard A.

    2013-03-01

    We demonstrate a comprehensive and broadly applicable methodology for the optimal in situ configuration of bendable soft x-ray Kirkpatrick-Baez mirrors. The mirrors used for this application are preset at the Advanced Light Source Optical Metrology Laboratory prior to beamline installation. The in situ methodology consists of a new technique for simultaneously setting the height and pitch angle of each mirror. The benders of both mirrors were then optimally tuned in order to minimize ray aberrations to a level below the diffraction-limited beam waist size of 200 nm (horizontal)×100 nm (vertical). After applying this methodology, we measured a beam waist size of 290 nm (horizontal)×130 nm (vertical) with 1 nm light using the Foucault knife-edge test. We also discuss the utility of using a grating-based lateral shearing interferometer with quantitative wavefront feedback for further improvement of bendable optics.

  19. Methodology for optimal in situ alignment and setting of bendable optics for diffraction-limited focusing of soft x-rays

    NASA Astrophysics Data System (ADS)

    Merthe, Daniel J.; Yashchuk, Valeriy V.; Goldberg, Kenneth A.; Kunz, Martin; Tamura, Nobumichi; McKinney, Wayne R.; Artemiev, Nikolay A.; Celestre, Richard S.; Morrison, Gregory Y.; Anderson, Erik; Smith, Brian V.; Domning, Edward E.; Rekawa, Senajith B.; Padmore, Howard A.

    2012-09-01

    We demonstrate a comprehensive and broadly applicable methodology for the optimal in situ configuration of bendable soft x-ray Kirkpatrick-Baez mirrors. The mirrors used for this application are preset at the ALS Optical Metrology Laboratory prior to beamline installation. The in situ methodology consists of a new technique for simultaneously setting the height and pitch angle of each mirror. The benders of both mirrors were then optimally tuned in order to minimize ray aberrations to a level below the diffraction-limited beam waist size of 200 nm (horizontal) × 100 nm (vertical). After applying this methodology, we measured a beam waist size of 290 nm (horizontal) × 130 nm (vertical) with 1 nm light using the Foucault knife-edge test. We also discuss the utility of using a grating-based lateral shearing interferometer with quantitative wavefront feedback for further improvement of bendable optics.

  20. Nearly diffraction-limited X-ray focusing with variable-numerical-aperture focusing optical system based on four deformable mirrors

    NASA Astrophysics Data System (ADS)

    Matsuyama, Satoshi; Nakamori, Hiroki; Goto, Takumi; Kimura, Takashi; Khakurel, Krishna P.; Kohmura, Yoshiki; Sano, Yasuhisa; Yabashi, Makina; Ishikawa, Tetsuya; Nishino, Yoshinori; Yamauchi, Kazuto

    2016-04-01

    Unlike the electrostatic and electromagnetic lenses used in electron microscopy, most X-ray focusing optical systems have fixed optical parameters with constant numerical apertures (NAs). This lack of adaptability has significantly limited application targets. In the research described herein, we developed a variable-NA X-ray focusing system based on four deformable mirrors, two sets of Kirkpatrick-Baez-type focusing mirrors, in order to control the focusing size while keeping the position of the focus unchanged. We applied a mirror deformation procedure using optical/X-ray metrology for offline/online adjustments. We performed a focusing test at a SPring-8 beamline and confirmed that the beam size varied from 108 nm to 560 nm (165 nm to 1434 nm) in the horizontal (vertical) direction by controlling the NA while maintaining diffraction-limited conditions.

  1. Sub-wavelength resolution of cracks in metallic materials

    NASA Astrophysics Data System (ADS)

    Amireddy, Kiran Kumar; Rajagopal, Prabhu; Balasubramaniam, Krishnan

    2017-02-01

    In recent years, various types of acoustic metamaterials have been proposed with capabilities for overcoming the diffraction limit. However, typically such developments only consider the acoustic regime or imaging in liquid media. In this paper we show the application of a holey structured metamaterial lens for sub-wavelength imaging of defects in a metallic sample, in the ultrasonic regime. Finite Element (FE) simulations are used to study longitudinal wave interaction with ideal cracks in isotropic elastic materials. Holey-structured meta-lenses are then used to transmit the scattered waves. We present a super resolution of λ/7 with a subwavelength crack in an aluminium sample, which to the best of our knowledge this is the highest resolution achieved in the ultrasonic regime.

  2. Super-Resolution Imaging and Plasmonics.

    PubMed

    Willets, Katherine A; Wilson, Andrew J; Sundaresan, Vignesh; Joshi, Padmanabh B

    2017-01-13

    This review describes the growing partnership between super-resolution imaging and plasmonics, by describing the various ways in which the two topics mutually benefit one another to enhance our understanding of the nanoscale world. First, localization-based super-resolution imaging strategies, where molecules are modulated between emissive and nonemissive states and their emission localized, are applied to plasmonic nanoparticle substrates, revealing the hidden shape of the nanoparticles while also mapping local electromagnetic field enhancements and reactivity patterns on their surface. However, these results must be interpreted carefully due to localization errors induced by the interaction between metallic substrates and single fluorophores. Second, plasmonic nanoparticles are explored as image contrast agents for both superlocalization and super-resolution imaging, offering benefits such as high photostability, large signal-to-noise, and distance-dependent spectral features but presenting challenges for localizing individual nanoparticles within a diffraction-limited spot. Finally, the use of plasmon-tailored excitation fields to achieve subdiffraction-limited spatial resolution is discussed, using localized surface plasmons and surface plasmon polaritons to create confined excitation volumes or image magnification to enhance spatial resolution.

  3. Virtual-'light-sheet' single-molecule localisation microscopy enables quantitative optical sectioning for super-resolution imaging.

    PubMed

    Palayret, Matthieu; Armes, Helen; Basu, Srinjan; Watson, Adam T; Herbert, Alex; Lando, David; Etheridge, Thomas J; Endesfelder, Ulrike; Heilemann, Mike; Laue, Ernest; Carr, Antony M; Klenerman, David; Lee, Steven F

    2015-01-01

    Single-molecule super-resolution microscopy allows imaging of fluorescently-tagged proteins in live cells with a precision well below that of the diffraction limit. Here, we demonstrate 3D sectioning with single-molecule super-resolution microscopy by making use of the fitting information that is usually discarded to reject fluorophores that emit from above or below a virtual-'light-sheet', a thin volume centred on the focal plane of the microscope. We describe an easy-to-use routine (implemented as an open-source ImageJ plug-in) to quickly analyse a calibration sample to define and use such a virtual light-sheet. In addition, the plug-in is easily usable on almost any existing 2D super-resolution instrumentation. This optical sectioning of super-resolution images is achieved by applying well-characterised width and amplitude thresholds to diffraction-limited spots that can be used to tune the thickness of the virtual light-sheet. This allows qualitative and quantitative imaging improvements: by rejecting out-of-focus fluorophores, the super-resolution image gains contrast and local features may be revealed; by retaining only fluorophores close to the focal plane, virtual-'light-sheet' single-molecule localisation microscopy improves the probability that all emitting fluorophores will be detected, fitted and quantitatively evaluated.

  4. High-resolution surface charge image achieved by a multiforce sensor based on a quartz tuning fork in electrostatic force microscope

    NASA Astrophysics Data System (ADS)

    Wang, Zhi-yong; Bao, Jian-bin; Zhang, Hong-hai; Guo, Wen-ming

    2002-08-01

    A multiforce sensor was fabricated by attaching a tiny tungsten tip to a tuning fork. By operating an ac modulation bias on the minitip of the needle sensor, we have achieved a dynamic noncontact mode electrostatic force microscope with high spatial resolution. It can utilize the van der Waals force and electrostatic force signals between the microtip and the sample, respectively, to obtain the images of topography and quantitative surface charge density of an open-gate field effect transistor simultaneously.

  5. Achieving Superior Tropical Cyclone Intensity Forecasts by Improving the Assimilation of High-Resolution Satellite Data into Mesoscale Prediction Models

    DTIC Science & Technology

    2013-09-30

    intensity analyses/predictions is being assessed. The main science focus is on investigating and understanding how the assimilation of the satellite...the impact of the enhanced AMV observations at high model resolution (i.e. ɜkm spacing). It involves sampling an ensemble of bogus vortices from...specifically for implementation as a hybrid data assimilation system using the Weather Research and Forecast (WRF) model. Among many other advantages

  6. A method for achieving an order-of-magnitude increase in the temporal resolution of a standard CRT computer monitor.

    PubMed

    Fiesta, Matthew P; Eagleman, David M

    2008-09-15

    As the frequency of a flickering light is increased, the perception of flicker is replaced by the perception of steady light at what is known as the critical flicker fusion threshold (CFFT). This threshold provides a useful measure of the brain's information processing speed, and has been used in medicine for over a century both for diagnostic and drug efficacy studies. However, the hardware for presenting the stimulus has not advanced to take advantage of computers, largely because the refresh rates of typical monitors are too slow to provide fine-grained changes in the alternation rate of a visual stimulus. For example, a cathode ray tube (CRT) computer monitor running at 100Hz will render a new frame every 10 ms, thus restricting the period of a flickering stimulus to multiples of 20 ms. These multiples provide a temporal resolution far too low to make precise threshold measurements, since typical CFFT values are in the neighborhood of 35 ms. We describe here a simple and novel technique to enable alternating images at several closely-spaced periods on a standard monitor. The key to our technique is to programmatically control the video card to dynamically reset the refresh rate of the monitor. Different refresh rates allow slightly different frame durations; this can be leveraged to vastly increase the resolution of stimulus presentation times. This simple technique opens new inroads for experiments on computers that require more finely-spaced temporal resolution than a monitor at a single, fixed refresh rate can allow.

  7. Compact tunable diode laser with diffraction limited 1000 mW in Littman/Metcalf configuration for cavity ring down spectroscopy

    NASA Astrophysics Data System (ADS)

    Stry, Sandra; Sacher, Joachim; Thelen, Sven; Halmer, Daniel; Hering, Peter; Mürtz, Manfred

    2006-02-01

    High resolution spectroscopy of environmental and medical gases requires reliable, fast tunable laser light sources in the mid-infrared (MIR) wavelength regime between 3 and 5 μm. Since this wavelength cannot be reached via direct emitting room temperature semiconductor lasers, additional techniques like difference frequency generation (DFG) are essential. Tunable difference frequency generation relies on high power, small linewidth, fast tunable, robust laser diode sources. We report a new, very compact, alignment insensitive, robust, external cavity diode laser system in Littman/Metcalf configuration with an output power of 1000 mW and an almost Gaussian shaped beam quality (M2<1.2). The coupling efficiency for optical waveguides as well as single mode fibers exceeds 70%. The center wavelength is widely tunable within the tuning range of 20 nm via remote control. This laser system operates longitudinally single mode with a mode-hop free tuning range of up to 150 GHz without current compensation and a side-mode-suppression better than 50 dB. This concept can be realized within the wavelength regime between 750 and 1060 nm. We investigated this light source for high resolution spectroscopy in the field of Cavity Ring-Down Spectroscopy (CRDS). Our high powered Littman/Metcalf laser system was part of a MIR-light source which utilizes difference-frequency generation in Periodically Poled Lithium Niobate (PPLN) crystals. At the wavelength of 3.3 μm we were able to achieve a high-resolution absorption spectrum of water with four resolved isotopic H IIO components. This application clearly demonstrates the suitability of this laser for high-precision measurements.

  8. Phase stretch transform for super-resolution localization microscopy

    PubMed Central

    Ilovitsh, Tali; Jalali, Bahram; Asghari, Mohammad H.; Zalevsky, Zeev

    2016-01-01

    Super-resolution localization microscopy has revolutionized the observation of living structures at the cellular scale, by achieving a spatial resolution that is improved by more than an order of magnitude compared to the diffraction limit. These methods localize single events from isolated sources in repeated cycles in order to achieve super-resolution. The requirement for sparse distribution of simultaneously activated sources in the field of view dictates the acquisition of thousands of frames in order to construct the full super-resolution image. As a result, these methods have slow temporal resolution which is a major limitation when investigating live-cell dynamics. In this paper we present the use of a phase stretch transform for high-density super-resolution localization microscopy. This is a nonlinear frequency dependent transform that emulates the propagation of light through a physical medium with a specific warped diffractive property and applies a 2D phase function to the image in the frequency domain. By choosing properly the transform parameters and the phase kernel profile, the point spread function of each emitter can be sharpened and narrowed. This enables the localization of overlapping emitters, thus allowing a higher density of activated emitters as well as shorter data collection acquisition rates. The method is validated by numerical simulations and by experimental data obtained using a microtubule sample. PMID:27867725

  9. Near-field terahertz probes with room-temperature nanodetectors for subwavelength resolution imaging

    PubMed Central

    Mitrofanov, Oleg; Viti, Leonardo; Dardanis, Enrico; Giordano, Maria Caterina; Ercolani, Daniele; Politano, Antonio; Sorba, Lucia; Vitiello, Miriam S.

    2017-01-01

    Near-field imaging with terahertz (THz) waves is emerging as a powerful technique for fundamental research in photonics and across physical and life sciences. Spatial resolution beyond the diffraction limit can be achieved by collecting THz waves from an object through a small aperture placed in the near-field. However, light transmission through a sub-wavelength size aperture is fundamentally limited by the wave nature of light. Here, we conceive a novel architecture that exploits inherently strong evanescent THz field arising within the aperture to mitigate the problem of vanishing transmission. The sub-wavelength aperture is originally coupled to asymmetric electrodes, which activate the thermo-electric THz detection mechanism in a transistor channel made of flakes of black-phosphorus or InAs nanowires. The proposed novel THz near-field probes enable room-temperature sub-wavelength resolution coherent imaging with a 3.4 THz quantum cascade laser, paving the way to compact and versatile THz imaging systems and promising to bridge the gap in spatial resolution from the nanoscale to the diffraction limit. PMID:28287123

  10. Near-field terahertz probes with room-temperature nanodetectors for subwavelength resolution imaging

    NASA Astrophysics Data System (ADS)

    Mitrofanov, Oleg; Viti, Leonardo; Dardanis, Enrico; Giordano, Maria Caterina; Ercolani, Daniele; Politano, Antonio; Sorba, Lucia; Vitiello, Miriam S.

    2017-03-01

    Near-field imaging with terahertz (THz) waves is emerging as a powerful technique for fundamental research in photonics and across physical and life sciences. Spatial resolution beyond the diffraction limit can be achieved by collecting THz waves from an object through a small aperture placed in the near-field. However, light transmission through a sub-wavelength size aperture is fundamentally limited by the wave nature of light. Here, we conceive a novel architecture that exploits inherently strong evanescent THz field arising within the aperture to mitigate the problem of vanishing transmission. The sub-wavelength aperture is originally coupled to asymmetric electrodes, which activate the thermo-electric THz detection mechanism in a transistor channel made of flakes of black-phosphorus or InAs nanowires. The proposed novel THz near-field probes enable room-temperature sub-wavelength resolution coherent imaging with a 3.4 THz quantum cascade laser, paving the way to compact and versatile THz imaging systems and promising to bridge the gap in spatial resolution from the nanoscale to the diffraction limit.

  11. Light funneling from a photonic crystal laser cavity to a nano-antenna: overcoming the diffraction limit in optical energy transfer down to the nanoscale.

    PubMed

    Mivelle, Mathieu; Viktorovitch, Pierre; Baida, Fadi I; El Eter, Ali; Xie, Zhihua; Vo, Than-Phong; Atie, Elie; Burr, Geoffrey W; Nedeljkovic, Dusan; Rauch, Jean-Yves; Callard, Ségolène; Grosjean, Thierry

    2014-06-16

    We show that the near-field coupling between a photonic crystal microlaser and a nano-antenna can enable hybrid photonic systems that are both physically compact (free from bulky optics) and efficient at transferring optical energy into the nano-antenna. Up to 19% of the laser power from a micron-scale photonic crystal laser cavity is experimentally transferred to a bowtie aperture nano-antenna (BNA) whose area is 400-fold smaller than the overall emission area of the microlaser. Instead of a direct deposition of the nano-antenna onto the photonic crystal, it is fabricated at the apex of a fiber tip to be accurately placed in the microlaser near-field. Such light funneling within a hybrid structure provides a path for overcoming the diffraction limit in optical energy transfer to the nanoscale and should thus open promising avenues in the nanoscale enhancement and confinement of light in compact architectures, impacting applications such as biosensing, optical trapping, local heating, spectroscopy, and nanoimaging.

  12. Widely tunable diffraction limited 1000 mW external cavity diode laser in Littman/Metcalf configuration for cavity ring-down spectroscopy

    NASA Astrophysics Data System (ADS)

    Stry, S.; Thelen, S.; Sacher, J.; Halmer, D.; Hering, P.; Mürtz, M.

    2006-11-01

    We report on recent progress on external cavity diode lasers (ECDL) using a new concept of a Littman/Metcalf configuration. Within this concept one facet of the diode laser chip is used for coupling to a high quality Littman/Metcalf resonator whereas the other side of the diode laser chip emits the output beam. The alignment of the external resonator is independent from the alignment of the output beam and there is no need for any compromise in the alignment. This results in an improved behavior of the external resonator with the benefit of a drastic increase in power and single mode tuning. We investigated this light source for high resolution spectroscopy in the field of cw-cavity ring-down spectroscopy (CRDS). The monitoring of environmental and medical gases from vehicles or human breath requires a suitable radiation source in the mid-infrared (MIR) between 3 and 5 μm that is frequency stable and can be widely tuned. Since this wavelength cannot be reached via direct emitting room temperature semiconductor lasers, additional techniques like difference frequency generation (DFG) are essential. Tunable difference frequency generation relies on high power, small linewidth, fast tunable, robust laser diode sources with excellent beam quality. With our new compact, alignment-insensitive and robust ECDL concept, we achieved an output power of 1000 mW and an almost Gaussian shaped beam quality (M2<1.2). The coupling efficiency for optical waveguides as well as single mode fibers exceeds 70%. The wavelength is widely tunable within the tuning range of 20 nm via remote control. This laser system operates longitudinally in single mode with a mode-hop free tuning range of more than 150 GHz without current compensation and a side-mode-suppression better than 50 dB. This concept is currently realized within the wavelength regime between 750 and 1080 nm. Our high powered Littman/Metcalf laser system was part of a MIR-light source which utilizes DFG in periodically poled

  13. Testing the limits of Paleozoic chronostratigraphic correlation via high-resolution (13Ccarb) biochemostratigraphy across the Llandovery–Wenlock (Silurian) boundary: Is a unified Phanerozoic time scale achievable?

    USGS Publications Warehouse

    Cramer, Bradley D.; Loydell, David K.; Samtleben, Christian; Munnecke, Axel; Kaljo, Dimitri; Mannik, Peep; Martma, Tonu; Jeppsson, Lennart; Kleffner, Mark A.; Barrick, James E.; Johnson, Craig A.; Emsbo, Poul; Joachimski, Michael M.; Bickert, Torsten; Saltzman, Matthew R.

    2010-01-01

    The resolution and fidelity of global chronostratigraphic correlation are direct functions of the time period under consideration. By virtue of deep-ocean cores and astrochronology, the Cenozoic and Mesozoic time scales carry error bars of a few thousand years (k.y.) to a few hundred k.y. In contrast, most of the Paleozoic time scale carries error bars of plus or minus a few million years (m.y.), and chronostratigraphic control better than ??1 m.y. is considered "high resolution." The general lack of Paleozoic abyssal sediments and paucity of orbitally tuned Paleozoic data series combined with the relative incompleteness of the Paleozoic stratigraphic record have proven historically to be such an obstacle to intercontinental chronostratigraphic correlation that resolving the Paleozoic time scale to the level achieved during the Mesozoic and Cenozoic was viewed as impractical, impossible, or both. Here, we utilize integrated graptolite, conodont, and carbonate carbon isotope (??13Ccarb) data from three paleocontinents (Baltica, Avalonia, and Laurentia) to demonstrate chronostratigraphic control for upper Llando very through middle Wenlock (Telychian-Sheinwoodian, ~436-426 Ma) strata with a resolution of a few hundred k.y. The interval surrounding the base of the Wenlock Series can now be correlated globally with precision approaching 100 k.y., but some intervals (e.g., uppermost Telychian and upper Shein-woodian) are either yet to be studied in sufficient detail or do not show sufficient biologic speciation and/or extinction or carbon isotopic features to delineate such small time slices. Although producing such resolution during the Paleozoic presents an array of challenges unique to the era, we have begun to demonstrate that erecting a Paleozoic time scale comparable to that of younger eras is achievable. ?? 2010 Geological Society of America.

  14. SU-E-J-126: Respiratory Gating Quality Assurance: A Simple Method to Achieve Millisecond Temporal Resolution

    SciTech Connect

    McCabe, B; Wiersma, R

    2014-06-01

    Purpose: Low temporal latency between a gating on/off signal and a linac beam on/off during respiratory gating is critical for patient safety. Although, a measurement of temporal lag is recommended by AAPM Task Group 142 for commissioning and annual quality assurance, there currently exists no published method. Here we describe a simple, inexpensive, and reliable method to precisely measure gating lag at millisecond resolutions. Methods: A Varian Real-time Position Management™ (RPM) gating simulator with rotating disk was modified with a resistive flex sensor (Spectra Symbol) attached to the gating box platform. A photon diode was placed at machine isocenter. Output signals of the flex sensor and diode were monitored with a multichannel oscilloscope (Tektronix™ DPO3014). Qualitative inspection of the gating window/beam on synchronicity were made by setting the linac to beam on/off at end-expiration, and the oscilloscope's temporal window to 100 ms to visually examine if the on/off timing was within the recommended 100-ms tolerance. Quantitative measurements were made by saving the signal traces and analyzing in MatLab™. The on and off of the beam signal were located and compared to the expected gating window (e.g. 40% to 60%). Four gating cycles were measured and compared. Results: On a Varian TrueBeam™ STx linac with RPM gating software, the average difference in synchronicity at beam on and off for four cycles was 14 ms (3 to 30 ms) and 11 ms (2 to 32 ms), respectively. For a Varian Clinac™ 21EX the average difference at beam on and off was 127 ms (122 to 133 ms) and 46 ms (42 to 49 ms), respectively. The uncertainty in the synchrony difference was estimated at ±6 ms. Conclusion: This new gating QA method is easy to implement and allows for fast qualitative inspection and quantitative measurements for commissioning and TG-142 annual QA measurements.

  15. High-Resolution Solar Imaging With Photon Sieves

    NASA Astrophysics Data System (ADS)

    Oktem, F. S.; Kamalabadi, F.; Davila, J. M.

    2014-12-01

    A photon sieve is a modification of a Fresnel zone plate in which open zones are replaced by a large number of circular holes. This lightweight optical device offers a superior image forming capability compared with the Fresnel zone plate, and is specially suited to observations at UV and x-ray wavelengths where refractive lenses are not available due to strong absorption of materials, and reflective mirrors are difficult to manufacture to achieve near diffraction-limited resolution. At these shorter wavelengths, photon sieves enable diffraction-limited imaging performance with relaxed manufacturing tolerances, and simple and low-cost fabrication. In this work, we present a new photon sieve imaging modality that, unlike previous designs, takes advantage of chromatic aberration. The fact that different wavelengths are focused at different distances from photon sieve is exploited to develop a novel multi-spectral imaging technique. The idea is to use a photon sieve imaging system with a moving detector which records images at different planes. Each measurement consists of superimposed images of different wavelengths, with each individual image being either in focus or out of focus. For spatially incoherent illumination, we study the problem of recovering the individual images from these superimposed measurements. We first formulate the discrete forward problem using the closed-form Fresnel imaging formulas. The inverse problem is then a multi-frame deconvolution problem involving multiple objects, and is formulated as a maximum posterior estimation problem. The resulting nonlinear optimization problem is solved using a fixed-point iterative algorithm. In contrast to traditional spectral imagers employing a series of wavelength filters, the proposed technique relies on a simple optical system, but incorporates powerful image processing methods to form spectral images computationally. In addition to diffraction-limited high spatial resolution enabled by photon sieves

  16. A scanning, all-fiber Sagnac interferometer for high resolution magneto-optic measurements at 820 nm

    SciTech Connect

    Fried, Alexander; Fejer, Martin; Kapitulnik, Aharon

    2014-10-15

    The Sagnac Interferometer has historically been used for detecting non-reciprocal phenomena, such as rotation. We demonstrate an apparatus in which this technique is employed for high resolution measurements of the Magneto-Optical Polar Kerr effect—a direct indicator of magnetism. Previous designs have incorporated free-space components which are bulky and difficult to align. We improve upon this technique by using all fiber-optic coupled components and demonstrate operation at a new wavelength, 820 nm, with which we can achieve better than 1 μrad resolution. Mounting the system on a piezo-electric scanner allows us to acquire diffraction limited images with 1.5 μm spatial resolution. We also provide extensive discussion on the details and of the Sagnac Interferometer's construction.

  17. STED-like resolution enhancement with focus extension

    NASA Astrophysics Data System (ADS)

    Yang, Kai-Ping; Lin, Wei-Kuan; Hsu, Kuo-Jen; Lin, Yen-Yin; Chu, Shi-Wei

    2016-03-01

    In recent years, the techniques of super-resolution have generated widespread impacts in science. Stimulated emission depletion (STED) microscopy is known for achieving sub-diffraction-limit resolution by using a donut-shaped beam to deplete the fluorescence around a focal spot while leaving a central part active to emit fluorescence. However, since STED microscopy is based on fluorescence, it suffers from photo-bleaching. We recently developed a new technique and termed it as suppression of scattering imaging (SUSI) microscopy. It uses a STED-like setup and achieves super resolution imaging by utilizing the nonlinearity of scattering from gold nanoparticles. Therefore, SUSI microscopy avoids the photo-bleaching issue. Nonetheless, for fast volumetric imaging, SUSI microscopy is limited with slow axial translation of the objective or sample. Here we combine SUSI microscopy with a refractive-index-variable lens to axially move the focus at very high speed. This combination allows simultaneous observation of tissue dynamics over a three-dimensional volume within one second. The new technique paves the way toward high-speed super-resolution imaging for biological tissues.

  18. Fast Vascular Ultrasound Imaging with Enhanced Spatial Resolution and Background Rejection.

    PubMed

    Bar-Zion, Avinoam; Tremblay-Darveau, Charles; Solomon, Oren; Adam, Dan; Eldar, Yonina

    2016-08-15

    Ultrasound super-localization microscopy techniques presented in the last few years enable non-invasive imaging of vascular structures at the capillary level by tracking the flow of ultrasound contrast agents (gas microbubbles). However, these techniques are currently limited by low temporal resolution and long acquisition times. Super-resolution optical fluctuation imaging (SOFI) is a fluorescence microscopy technique enabling sub-diffraction limit imaging with high temporal resolution by calculating high order statistics of the fluctuating optical signal. The aim of this work is to achieve fast acoustic imaging with enhanced resolution by applying the tools used in SOFI to contrast-enhance ultrasound (CEUS) plane-wave scans. The proposed method was tested using numerical simulations and evaluated using two in-vivo rabbit models: scans of healthy kidneys and VX-2 tumor xenografts. Improved spatial resolution was observed with a reduction of up to 50% in the full width half max of the point spread function. In addition, substantial reduction in the background level was achieved compared to standard mean amplitude persistence images, revealing small vascular structures within tumors. The scan duration of the proposed method is less than a second while current superlocalization techniques require acquisition duration of several minutes. As a result, the proposed technique may be used to obtain scans with enhanced spatial resolution and high temporal resolution, facilitating flow-dynamics monitoring. Our method can also be applied during a breath-hold, reducing the sensitivity to motion artifacts.

  19. Fast Vascular Ultrasound Imaging With Enhanced Spatial Resolution and Background Rejection.

    PubMed

    Bar-Zion, Avinoam; Tremblay-Darveau, Charles; Solomon, Oren; Adam, Dan; Eldar, Yonina C

    2017-01-01

    Ultrasound super-localization microscopy techniques presented in the last few years enable non-invasive imaging of vascular structures at the capillary level by tracking the flow of ultrasound contrast agents (gas microbubbles). However, these techniques are currently limited by low temporal resolution and long acquisition times. Super-resolution optical fluctuation imaging (SOFI) is a fluorescence microscopy technique enabling sub-diffraction limit imaging with high temporal resolution by calculating high order statistics of the fluctuating optical signal. The aim of this work is to achieve fast acoustic imaging with enhanced resolution by applying the tools used in SOFI to contrast-enhance ultrasound (CEUS) plane-wave scans. The proposed method was tested using numerical simulations and evaluated using two in-vivo rabbit models: scans of healthy kidneys and VX-2 tumor xenografts. Improved spatial resolution was observed with a reduction of up to 50% in the full width half max of the point spread function. In addition, substantial reduction in the background level was achieved compared to standard mean amplitude persistence images, revealing small vascular structures within tumors. The scan duration of the proposed method is less than a second while current super-localization techniques require acquisition duration of several minutes. As a result, the proposed technique may be used to obtain scans with enhanced spatial resolution and high temporal resolution, facilitating flow-dynamics monitoring. Our method can also be applied during a breath-hold, reducing the sensitivity to motion artifacts.

  20. Memory-effect based deconvolution microscopy for super-resolution imaging through scattering media

    PubMed Central

    Edrei, Eitan; Scarcelli, Giuliano

    2016-01-01

    High-resolution imaging through turbid media is a fundamental challenge of optical sciences that has attracted a lot of attention in recent years for its wide range of potential applications. Here, we demonstrate that the resolution of imaging systems looking behind a highly scattering medium can be improved below the diffraction-limit. To achieve this, we demonstrate a novel microscopy technique enabled by the optical memory effect that uses a deconvolution image processing and thus it does not require iterative focusing, scanning or phase retrieval procedures. We show that this newly established ability of direct imaging through turbid media provides fundamental and practical advantages such as three-dimensional refocusing and unambiguous object reconstruction. PMID:27633483

  1. Memory-effect based deconvolution microscopy for super-resolution imaging through scattering media

    NASA Astrophysics Data System (ADS)

    Edrei, Eitan; Scarcelli, Giuliano

    2016-09-01

    High-resolution imaging through turbid media is a fundamental challenge of optical sciences that has attracted a lot of attention in recent years for its wide range of potential applications. Here, we demonstrate that the resolution of imaging systems looking behind a highly scattering medium can be improved below the diffraction-limit. To achieve this, we demonstrate a novel microscopy technique enabled by the optical memory effect that uses a deconvolution image processing and thus it does not require iterative focusing, scanning or phase retrieval procedures. We show that this newly established ability of direct imaging through turbid media provides fundamental and practical advantages such as three-dimensional refocusing and unambiguous object reconstruction.

  2. Adaptive optics high resolution spectroscopy: present status and future direction

    SciTech Connect

    Alcock, C; Angel, R; Ciarlo, D; Fugate, R O; Ge, J; Kuzmenko, P; Lloyd-Hart, M; Macintosh, B; Najita, J; Woolf, N

    1999-07-27

    High resolution spectroscopy experiments with visible adaptive optics (AO) telescopes at Starfire Optical Range and Mt. Wilson have demonstrated that spectral resolution can be routinely improved by a factor of - 10 over the seeing-limited case with no extra light losses at visible wavelengths. With large CCDs now available, a very wide wavelength range can be covered in a single exposure. In the near future, most large ground-based telescopes will be equipped with powerful A0 systems. Most of these systems are aimed primarily at diffraction-limited operation in the near IR. An exciting new opportunity will thus open up for high resolution IR spectroscopy. Immersion echelle gratings with much coarser grooves being developed by us at LLNL will play a critical role in achieving high spectral resolution with a compact and low cost IR cryogenically cooled spectrograph and simultaneous large wavelength coverage on relatively small IR detectors. We have constructed a new A0 optimized spectrograph at Steward Observatory to provide R = 200,000 in the optical, which is being commissioned at the Starfire Optical Range 3.5m telescope. We have completed the optical design of the LLNL IR Immersion Spectrograph (LISPEC) to take advantage of improved silicon etching technology. Key words: adaptive optics, spectroscopy, high resolution, immersion gratings

  3. Experimental study of the maximum resolution and packing density achievable in sintered and non-sintered binder-jet 3D printed steel microchannels

    SciTech Connect

    Elliott, Amy M; Mehdizadeh Momen, Ayyoub; Benedict, Michael; Kiggans Jr, James O

    2015-01-01

    Developing high resolution 3D printed metallic microchannels is a challenge especially when there is an essential need for high packing density of the primary material. While high packing density could be achieved by heating the structure to the sintering temperature, some heat sensitive applications require other strategies to improve the packing density of primary materials. In this study the goal is to develop high green or pack densities microchannels on the scale of 2-300 microns which have a robust mechanical structure. Binder-jet 3D printing is an additive manufacturing process in which droplets of binder are deposited via inkjet into a bed of powder. By repeatedly spreading thin layers of powder and depositing binder into the appropriate 2D profiles, complex 3D objects can be created one layer at time. Microchannels with features on the order of 500 microns were fabricated via binder jetting of steel powder and then sintered and/or infiltrated with a secondary material. The average particle size of the steel powder was varied along with the droplet volume of the inkjet-deposited binder. The resolution of the process, packing density of the primary material, the subsequent features sizes of the microchannels, and the overall microchannel quality were characterized as a function of particle size distribution, droplet sizes and heat treatment temperatures.

  4. In patients with extensive subcutaneous emphysema, which technique achieves maximal clinical resolution: infraclavicular incisions, subcutaneous drain insertion or suction on in situ chest drain?

    PubMed

    Johnson, Charles H N; Lang, Sommer A; Bilal, Haris; Rammohan, Kandadai S

    2014-06-01

    A best evidence topic in cardiac surgery was written according to a structured protocol. The question addressed was: 'In patients with extensive subcutaneous emphysema, which technique achieves maximal clinical resolution: infraclavicular incisions, subcutaneous drain insertion or suction on in situ chest drain?'. Altogether more than 200 papers were found using the reported search, of which 14 represented the best evidence to answer the clinical question. The authors, journal, date and country of publication, patient group studied, study type, relevant outcomes and results of these papers are tabulated. Subcutaneous emphysema is usually a benign, self-limiting condition only requiring conservative management. Interventions are useful in the context of severe patient discomfort, respiratory distress or persistent air leak. In the absence of any comparative study, it is not possible to choose definitively between infraclavicular incisions, drain insertion and increasing suction on an in situ drain as the best method for managing severe subcutaneous emphysema. All the three techniques described have been shown to provide effective relief. Increasing suction on a chest tube already in situ provided rapid relief in patients developing SE following pulmonary resection. A retrospective study showed resolution in 66%, increasing to 98% in those who underwent video-assisted thoracic surgery with identification and closure of the leak. Insertion of a drain into the subcutaneous tissue also provided rapid sustained relief. Several studies aided drainage by using regular compressive massage. Infraclavicular incisions were also shown to provide rapid relief, but were noted to be more invasive and carried the potential for cosmetic defect. No major complications were illustrated.

  5. A super-resolution ultrasound method for brain vascular mapping

    PubMed Central

    O'Reilly, Meaghan A.; Hynynen, Kullervo

    2013-01-01

    Purpose: High-resolution vascular imaging has not been achieved in the brain due to limitations of current clinical imaging modalities. The authors present a method for transcranial ultrasound imaging of single micrometer-size bubbles within a tube phantom. Methods: Emissions from single bubbles within a tube phantom were mapped through an ex vivo human skull using a sparse hemispherical receiver array and a passive beamforming algorithm. Noninvasive phase and amplitude correction techniques were applied to compensate for the aberrating effects of the skull bone. The positions of the individual bubbles were estimated beyond the diffraction limit of ultrasound to produce a super-resolution image of the tube phantom, which was compared with microcomputed tomography (micro-CT). Results: The resulting super-resolution ultrasound image is comparable to results obtained via the micro-CT for small tissue specimen imaging. Conclusions: This method provides superior resolution to deep-tissue contrast ultrasound and has the potential to be extended to provide complete vascular network imaging in the brain. PMID:24320408

  6. Correcting chromatic offset in multicolor super-resolution localization microscopy.

    PubMed

    Erdelyi, Miklos; Rees, Eric; Metcalf, Daniel; Schierle, Gabriele S Kaminski; Dudas, Laszlo; Sinko, Jozsef; Knight, Alex E; Kaminski, Clemens F

    2013-05-06

    Localization based super-resolution microscopy techniques require precise drift correction methods because the achieved spatial resolution is close to both the mechanical and optical performance limits of modern light microscopes. Multi-color imaging methods require corrections in addition to those dealing with drift due to the static, but spatially-dependent, chromatic offset between images. We present computer simulations to quantify this effect, which is primarily caused by the high-NA objectives used in super-resolution microscopy. Although the chromatic offset in well corrected systems is only a fraction of an optical wavelength in magnitude (<50 nm) and thus negligible in traditional diffraction limited imaging, we show that object colocalization by multi-color super-resolution methods is impossible without appropriate image correction. The simulated data are in excellent agreement with experiments using fluorescent beads excited and localized at multiple wavelengths. Finally we present a rigorous and practical calibration protocol to correct for chromatic optical offset, and demonstrate its efficacy for the imaging of transferrin receptor protein colocalization in HeLa cells using two-color direct stochastic optical reconstruction microscopy (dSTORM).

  7. Super-Resolution Imaging at Mid-Infrared Waveband in Graphene-nanocavity formed on meta-surface

    PubMed Central

    Yang, Jingzhong; Wang, Taisheng; Chen, Zuolong; Hu, Bingliang; Yu, Weixing

    2016-01-01

    Plasmonic structured illumination microscopy (PSIM) is one of the promising wide filed optical imaging methods, which takes advantage of the surface plasmons to break the optical diffraction limit and thus to achieve a super-resolution optical image. To further improve the imaging resolution of PSIM, we propose in this work a so called graphene nanocavity on meta-surface structure (GNMS) to excite graphene surface plasmons with a deep sub-wavelength at mid-infrared waveband. It is found that surface plasmonic interference pattern with a period of around 52 nm can be achieved in graphene nanocavity formed on structured meta-surface for a 7 μm wavelength incident light. Moreover, the periodic plasmonic interference pattern can be tuned by simply changing the nanostructures fabricated on meta-surface for different application purposes. At last, the proposed GNMS structure is applied for super-resolution imaging in PSIM and it is found that an imaging resolution of 26 nm can be achieved, which is nearly 100 folds higher than that can be achieved by conventional epi-fluorescence microscopy. In comparison with visible waveband, mid-infrared is more gently and safe to biological cells and thus this work opens the new possibility for optical super-resolution imaging at mid-infrared waveband for biological research field. PMID:27897207

  8. Super-Resolution Imaging at Mid-Infrared Waveband in Graphene-nanocavity formed on meta-surface

    NASA Astrophysics Data System (ADS)

    Yang, Jingzhong; Wang, Taisheng; Chen, Zuolong; Hu, Bingliang; Yu, Weixing

    2016-11-01

    Plasmonic structured illumination microscopy (PSIM) is one of the promising wide filed optical imaging methods, which takes advantage of the surface plasmons to break the optical diffraction limit and thus to achieve a super-resolution optical image. To further improve the imaging resolution of PSIM, we propose in this work a so called graphene nanocavity on meta-surface structure (GNMS) to excite graphene surface plasmons with a deep sub-wavelength at mid-infrared waveband. It is found that surface plasmonic interference pattern with a period of around 52 nm can be achieved in graphene nanocavity formed on structured meta-surface for a 7 μm wavelength incident light. Moreover, the periodic plasmonic interference pattern can be tuned by simply changing the nanostructures fabricated on meta-surface for different application purposes. At last, the proposed GNMS structure is applied for super-resolution imaging in PSIM and it is found that an imaging resolution of 26 nm can be achieved, which is nearly 100 folds higher than that can be achieved by conventional epi-fluorescence microscopy. In comparison with visible waveband, mid-infrared is more gently and safe to biological cells and thus this work opens the new possibility for optical super-resolution imaging at mid-infrared waveband for biological research field.

  9. Super-Resolution Imaging at Mid-Infrared Waveband in Graphene-nanocavity formed on meta-surface.

    PubMed

    Yang, Jingzhong; Wang, Taisheng; Chen, Zuolong; Hu, Bingliang; Yu, Weixing

    2016-11-29

    Plasmonic structured illumination microscopy (PSIM) is one of the promising wide filed optical imaging methods, which takes advantage of the surface plasmons to break the optical diffraction limit and thus to achieve a super-resolution optical image. To further improve the imaging resolution of PSIM, we propose in this work a so called graphene nanocavity on meta-surface structure (GNMS) to excite graphene surface plasmons with a deep sub-wavelength at mid-infrared waveband. It is found that surface plasmonic interference pattern with a period of around 52 nm can be achieved in graphene nanocavity formed on structured meta-surface for a 7 μm wavelength incident light. Moreover, the periodic plasmonic interference pattern can be tuned by simply changing the nanostructures fabricated on meta-surface for different application purposes. At last, the proposed GNMS structure is applied for super-resolution imaging in PSIM and it is found that an imaging resolution of 26 nm can be achieved, which is nearly 100 folds higher than that can be achieved by conventional epi-fluorescence microscopy. In comparison with visible waveband, mid-infrared is more gently and safe to biological cells and thus this work opens the new possibility for optical super-resolution imaging at mid-infrared waveband for biological research field.

  10. Two-photon instant structured illumination microscopy improves the depth penetration of super-resolution imaging in thick scattering samples.

    PubMed

    Winter, Peter W; York, Andrew G; Nogare, Damian Dalle; Ingaramo, Maria; Christensen, Ryan; Chitnis, Ajay; Patterson, George H; Shroff, Hari

    2014-09-20

    Fluorescence imaging methods that achieve spatial resolution beyond the diffraction limit (super-resolution) are of great interest in biology. We describe a super-resolution method that combines two-photon excitation with structured illumination microscopy (SIM), enabling three-dimensional interrogation of live organisms with ~150 nm lateral and ~400 nm axial resolution, at frame rates of ~1 Hz. By performing optical rather than digital processing operations to improve resolution, our microscope permits super-resolution imaging with no additional cost in acquisition time or phototoxicity relative to the point-scanning two-photon microscope upon which it is based. Our method provides better depth penetration and inherent optical sectioning than all previously reported super-resolution SIM implementations, enabling super-resolution imaging at depths exceeding 100 μm from the coverslip surface. The capability of our system for interrogating thick live specimens at high resolution is demonstrated by imaging whole nematode embryos and larvae, and tissues and organs inside zebrafish embryos.

  11. Surface plasmon resonance microscopy: Achieving a quantitative optical response

    NASA Astrophysics Data System (ADS)

    Peterson, Alexander W.; Halter, Michael; Plant, Anne L.; Elliott, John T.

    2016-09-01

    Surface plasmon resonance (SPR) imaging allows real-time label-free imaging based on index of refraction and changes in index of refraction at an interface. Optical parameter analysis is achieved by application of the Fresnel model to SPR data typically taken by an instrument in a prism based figuration. We carry out SPR imaging on a microscope by launching light into a sample and collecting reflected light through a high numerical aperture microscope objective. The SPR microscope enables spatial resolution that approaches the diffraction limit and has a dynamic range that allows detection of subnanometer to submicrometer changes in thickness of biological material at a surface. However, unambiguous quantitative interpretation of SPR changes using the microscope system could not be achieved using the Fresnel model because of polarization dependent attenuation and optical aberration that occurs in the high numerical aperture objective. To overcome this problem, we demonstrate a model to correct for polarization diattenuation and optical aberrations in the SPR data and develop a procedure to calibrate reflectivity to index of refraction values. The calibration and correction strategy for quantitative analysis was validated by comparing the known indices of refraction of bulk materials with corrected SPR data interpreted with the Fresnel model. Subsequently, we applied our SPR microscopy method to evaluate the index of refraction for a series of polymer microspheres in aqueous media and validated the quality of the measurement with quantitative phase microscopy.

  12. Super-resolution Microscopy Approaches for Live Cell Imaging

    PubMed Central

    Godin, Antoine G.; Lounis, Brahim; Cognet, Laurent

    2014-01-01

    By delivering optical images with spatial resolutions below the diffraction limit, several super-resolution fluorescence microscopy techniques opened new opportunities to study biological structures with details approaching molecular structure sizes. They have now become methods of choice for imaging proteins and their nanoscale dynamic organizations in live cells. In this mini-review, we describe and compare the main far-field super-resolution approaches that allow studying endogenous or overexpressed proteins in live cells. PMID:25418158

  13. Super-Resolution Real Imaging in Microsphere-Assisted Microscopy

    PubMed Central

    Wang, Feifei; Li, Yi; Jia, Boliang; Liu, Lianqing; Li, Wen Jung

    2016-01-01

    Microsphere-assisted microscopy has received a lot of attention recently due to its simplicity and its capability to surpass the diffraction limit. However, to date, sub-diffraction-limit features have only been observed in virtual images formed through the microspheres. We show that it is possible to form real, super-resolution images using high-refractive index microspheres. Also, we report on how changes to a microsphere’s refractive index and size affect image formation and planes. The relationship between the focus position and the additional magnification factor is also investigated using experimental and theoretical methods. We demonstrate that such a real imaging mode, combined with the use of larger microspheres, can enlarge sub-diffraction-limit features up to 10 times that of wide-field microscopy’s magnification with a field-of-view diameter of up to 9 μm. PMID:27768774

  14. Achieving high-resolution soft-tissue imaging with cone-beam CT: a two-pronged approach for modulation of x-ray fluence and detector gain

    NASA Astrophysics Data System (ADS)

    Graham, S. A.; Siewerdsen, J. H.; Moseley, D. J.; Keller, H.; Shkumat, N. A.; Jaffray, D. A.

    2005-04-01

    Cone-beam computed tomography (CBCT) presents a highly promising and challenging advanced application of flat-panel detectors (FPDs). The great advantage of this adaptable technology is in the potential for sub-mm 3D spatial resolution in combination with soft-tissue detectability. While the former is achieved naturally by CBCT systems incorporating modern FPD designs (e.g., 200 - 400 um pixel pitch), the latter presents a significant challenge due to limitations in FPD dynamic range, large field of view, and elevated levels of x-ray scatter in typical CBCT configurations. We are investigating a two-pronged strategy to maximizing soft-tissue detectability in CBCT: 1) front-end solutions, including novel beam modulation designs (viz., spatially varying compensators) that alleviate detector dynamic range requirements, reduce x-ray scatter, and better distribute imaging dose in a manner suited to soft-tissue visualization throughout the field of view; and 2) back-end solutions, including implementation of an advanced FPD design (Varian PaxScan 4030CB) that features dual-gain and dynamic gain switching that effectively extends detector dynamic range to 18 bits. These strategies are explored quantitatively on CBCT imaging platforms developed in our laboratory, including a dedicated CBCT bench and a mobile isocentric C-arm (Siemens PowerMobil). Pre-clinical evaluation of improved soft-tissue visibility was carried out in phantom and patient imaging with the C-arm device. Incorporation of these strategies begin to reveal the full potential of CBCT for soft-tissue visualization, an essential step in realizing broad utility of this adaptable technology for diagnostic and image-guided procedures.

  15. Novel optical super-resolution pattern with upright edges diffracted by a tiny thin aperture.

    PubMed

    Wu, Jiu Hui; Zhou, Kejiang

    2015-08-24

    In the past decade numerous efforts have been concentrated to achieve optical imaging resolution beyond the diffraction limit. In this letter a thin microcavity theory of near-field optics is proposed by using the power flow theorem firstly. According to this theory, the near-field optical diffraction from a tiny aperture whose diameter is less than one-tenth incident wavelength embedded in a thin conducting film is investigated by considering this tiny aperture as a thin nanocavity. It is very surprising that there exists a kind of novel super-resolution diffraction patterns showing resolution better than λ/80 (λ is the incident wavelength), which is revealed for the first time to our knowledge in this letter. The mechanism that has allowed the imaging with this kind of super-resolution patterns is due to the interaction between the incident wave and the thin nanocavity with a complex wavenumber. More precisely, these super-resolution patterns with discontinuous upright peaks are formed by one or three items of the integration series about the cylindrical waves according to our simulation results. This novel optical super-resolution with upright edges by using the thin microcavity theory presented in the study could have potential applications in the future semiconductor lithography process, nano-size laser-drilling technology, microscopy, optical storage, optical switch, and optical information processing.

  16. High-Resolution Imaging of Mercury Using Earth-based Facilities

    NASA Astrophysics Data System (ADS)

    Ksanfomality, L. V.

    2002-07-01

    With a view to the further development of the short exposure method with a CCD detector, new observations of the planet Mercury were carried out at the Abastumany Astrophysical Observatory, Republic of Georgia, from October 30 to November 8, 2001. Comparison with the previous data, as well as the results of data processing based on newly developed algorithms, points to considerable progress achieved in the technique for observing Mercury. In some cases, under very favorable atmospheric conditions, the resolution attained is close to the diffraction limit of the astronomic instrument used. For the first time, topographic features on Mercury's surface were reliably resolved. Features with linear sizes as small as 120 km are successfully identified in the disk center.

  17. Spatial Covariance Reconstructive (SCORE) Super-Resolution Fluorescence Microscopy

    PubMed Central

    Deng, Yi; Sun, Mingzhai; Lin, Pei-Hui; Ma, Jianjie; Shaevitz, Joshua W.

    2014-01-01

    Super-resolution fluorescence microscopy has become a powerful tool to resolve structural information that is not accessible to traditional diffraction-limited imaging techniques such as confocal microscopy. Stochastic optical reconstruction microscopy (STORM) and photoactivation localization microscopy (PALM) are promising super-resolution techniques due to their relative ease of implementation and instrumentation on standard microscopes. However, the application of STORM is critically limited by its long sampling time. Several recent works have been focused on improving the STORM imaging speed by making use of the information from emitters with overlapping point spread functions (PSF). In this work, we present a fast and efficient algorithm that takes into account the blinking statistics of independent fluorescence emitters. We achieve sub-diffraction lateral resolution of 100 nm from 5 to 7 seconds of imaging. Our method is insensitive to background and can be applied to different types of fluorescence sources, including but not limited to the organic dyes and quantum dots that we demonstrate in this work. PMID:24788039

  18. Spatial covariance reconstructive (SCORE) super-resolution fluorescence microscopy.

    PubMed

    Deng, Yi; Sun, Mingzhai; Lin, Pei-Hui; Ma, Jianjie; Shaevitz, Joshua W

    2014-01-01

    Super-resolution fluorescence microscopy has become a powerful tool to resolve structural information that is not accessible to traditional diffraction-limited imaging techniques such as confocal microscopy. Stochastic optical reconstruction microscopy (STORM) and photoactivation localization microscopy (PALM) are promising super-resolution techniques due to their relative ease of implementation and instrumentation on standard microscopes. However, the application of STORM is critically limited by its long sampling time. Several recent works have been focused on improving the STORM imaging speed by making use of the information from emitters with overlapping point spread functions (PSF). In this work, we present a fast and efficient algorithm that takes into account the blinking statistics of independent fluorescence emitters. We achieve sub-diffraction lateral resolution of 100 nm from 5 to 7 seconds of imaging. Our method is insensitive to background and can be applied to different types of fluorescence sources, including but not limited to the organic dyes and quantum dots that we demonstrate in this work.

  19. DIFFRACTION-LIMITED VISIBLE LIGHT IMAGES OF ORION TRAPEZIUM CLUSTER WITH THE MAGELLAN ADAPTIVE SECONDARY ADAPTIVE OPTICS SYSTEM (MagAO)

    SciTech Connect

    Close, L. M.; Males, J. R.; Morzinski, K.; Kopon, D.; Follette, K.; Rodigas, T. J.; Hinz, P.; Wu, Y-L.; Puglisi, A.; Esposito, S.; Riccardi, A.; Pinna, E.; Xompero, M.; Briguglio, R.; Uomoto, A; Hare, T.

    2013-09-10

    We utilized the new high-order (250-378 mode) Magellan Adaptive Optics system (MagAO) to obtain very high spatial resolution observations in ''visible light'' with MagAO's VisAO CCD camera. In the good-median seeing conditions of Magellan (0.''5-0.''7), we find MagAO delivers individual short exposure images as good as 19 mas optical resolution. Due to telescope vibrations, long exposure (60 s) r' (0.63 {mu}m) images are slightly coarser at FWHM = 23-29 mas (Strehl {approx}28%) with bright (R < 9 mag) guide stars. These are the highest resolution filled-aperture images published to date. Images of the young ({approx}1 Myr) Orion Trapezium {theta}{sup 1} Ori A, B, and C cluster members were obtained with VisAO. In particular, the 32 mas binary {theta}{sup 1} Ori C{sub 1} C{sub 2} was easily resolved in non-interferometric images for the first time. The relative positions of the bright trapezium binary stars were measured with {approx}0.6-5 mas accuracy. We are now sensitive to relative proper motions of just {approx}0.2 mas yr{sup -1} ({approx}0.4 km s{sup -1} at 414 pc)-this is a {approx}2-10 Multiplication-Sign improvement in orbital velocity accuracy compared to previous efforts. For the first time, we see clear motion of the barycenter of {theta}{sup 1} Ori B{sub 2} B{sub 3} about {theta}{sup 1} Ori B{sub 1}. All five members of the {theta}{sup 1} Ori B system appear likely to be a gravitationally bound ''mini cluster'', but we find that not all the orbits can be both circular and co-planar. The lowest mass member of the {theta}{sup 1} Ori B system (B{sub 4}; mass {approx}0.2 M{sub Sun }) has a very clearly detected motion (at 4.1 {+-} 1.3 km s{sup -1}; correlation = 99.9%) w.r.t. B{sub 1}. Previous work has suggested that B{sub 4} and B{sub 3} are on long-term unstable orbits and will be ejected from this ''mini cluster''. However, our new ''baseline'' model of the {theta}{sup 1} Ori B system suggests a more hierarchical system than previously thought, and so

  20. Diode-pumped dual-wavelength Nd:LSO laser at 1059 and 1067  nm with nearly diffraction-limited beam quality.

    PubMed

    Huang, Xiaoxu; Lan, Jinglong; Lin, Zhi; Wang, Yi; Xu, Bin; Xu, Huiying; Cai, Zhiping; Xu, Xiaodong; Zhang, Jian; Xu, Jun

    2016-04-10

    We report a diode-pumped continuous-wave simultaneous dual-wavelength Nd:LSO laser at 1059 and 1067 nm. By employing a specially coated output coupler with relatively high transmissions at high-gain emission lines of 1075 and 1079 nm, the two low-gain emission lines, 1059 and 1067 nm, can be achieved, for the first time to our knowledge, with maximum output power of 1.27 W and slope efficiency of about 29.2%. The output power is only limited by the available pump power. Output beam quality is also measured to be about 1.19 and 1.21 of the beam propagation factors in the x and y directions, respectively.

  1. Hyperlenses and metalenses for far-field super-resolution imaging.

    PubMed

    Lu, Dylan; Liu, Zhaowei

    2012-01-01

    The resolution of conventional optical lens systems is always hampered by the diffraction limit. Recent developments in artificial metamaterials provide new avenues to build hyperlenses and metalenses that are able to image beyond the diffraction limit. Hyperlenses project super-resolution information to the far field through a magnification mechanism, whereas metalenses not only super-resolve subwavelength details but also enable optical Fourier transforms. Recently, there have been numerous designs for hyperlenses and metalenses, bringing fresh theoretical and experimental advances, though future directions and challenges remain to be overcome.

  2. Image amplification based super-resolution reconstruction procedure designed for wavefront-coded imaging system

    NASA Astrophysics Data System (ADS)

    Zhao, Hui; Zong, Caihui; Wei, Jingxuan; Xie, Xiaopeng

    2016-10-01

    Wave-front coding, proposed by Dowski and Cathey in 1995, is widely known to be capable of extending the depth of focus (DOF) of incoherent imaging systems. However, benefiting from its very large point spread function (PSF) generated by a suitably designed phase mask that is added to the aperture plane, wave-front coding could also be used to achieve super-resolution without replacing the current sensor with one of smaller pitch size. An image amplification based super-resolution reconstruction procedure has been specifically designed for wave-front coded imaging systems and its effectiveness has been tested by experiment. For instance, for a focal length of 50 mm and f-number 4.5, objects within the range [5 m, ∞] are clearly imaged with the help of wave-front coding, which indicates a DOF extension ratio of approximately 20. The proposed super-resolution reconstruction procedure produces at least 3× resolution improvement, with the quality of the reconstructed super-resolution image approaching the diffraction limit.

  3. Refocusing resolution based on negative refractive-photonic crystal group with Ag defects for target detection and imaging

    NASA Astrophysics Data System (ADS)

    Lian, Yingfei; Zhu, Na; Fang, Yuntuan; Sun, Jiwen; Chen, Junlv; Qian, Huili

    2015-03-01

    Negative refractive-photonic crystal (NR-PC) lenses that can exceed the diffraction limit of focus resolution for imaging and target detection in the near field have gotten more and more special attention in recent years. Three flat lens groups with Ag defects based on NR-PC are designed, and the focusing imaging in the NR-PC three flat lens groups is concluded with the extension of Snell's law, and the influence on the resolution for a target detection dynamic scanning scheme is simulated by using the finite difference time domain method. An optimal-doped structure with Ag defects is achieved by different simulation combinations. The refocusing resolution 0.18834λ is achieved in the optimal structure and there is approximately a 0.06806λ improvement in the refocusing resolution compared to those undoped with Ag (0.2564λ) it also possesses distinct smaller side-lobes than a single flat lens doped with Ag. This means the optimal detecting ability for the three NR-PC flat lens groups with Ag defects is more improved than that for a single undoped and doped with Ag. This is significant for the perfect imaging being achieved for a particle structure.

  4. Solid-immersion fluorescence microscopy with increased emission and super resolution

    SciTech Connect

    Liau, Z. L.; Porter, J. M.; Liau, A. A.; Chen, J. J.; Salmon, W. C.; Sheu, S. S.

    2015-01-07

    We investigate solid-immersion fluorescence microscopy suitable for super-resolution nanotechnology and biological imaging, and have observed limit of resolution as small as 15 nm with microspheres, mitochondria, and chromatin fibers. We have further observed that fluorescence efficiency increases with excitation power density, implicating appreciable stimulated emission and increased resolution. We discuss potential advantages of the solid-immersion microscopy, including combined use with previously established super-resolution techniques for reaching deeper beyond the conventional diffraction limit.

  5. A resolution commending the achievements and recognizing the importance of the Alliance to Save Energy on the 35th anniversary of the incorporation of the Alliance.

    THOMAS, 112th Congress

    Sen. Warner, Mark R. [D-VA

    2012-03-26

    04/18/2012 Resolution agreed to in Senate without amendment and with a preamble by Unanimous Consent. (text: CR S2517) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  6. A Diffraction-limited Survey for Direct Detection of Halpha Emitting/Accreting ExtraSolar Planets with the 6.5m Magellan Telescope and the MagAO Visible AO system

    NASA Astrophysics Data System (ADS)

    Close, Laird

    steady diet of hydrogen gas. Such planets should then be quite bright in Halpha accretion emission. The key point is that: instead of a steep drop off in the luminosity of the planet’s atmosphere, the accretion luminosity of these planets will just linearly decrease with decreasing mass. At an accretion rate=6e-10 Msun/yr we find low mass (~1 Mjup) accreting gap planets are much (50-1000x) brighter (for 0-3.4 mag of Halpha extinction) in Halpha than at H band. PROOF-OF_CONCEPT: A 3 hour MagAO observation at Halpha of a transitional disk in April 2013 was made. The resulting deep diffraction-limited images discovered (at 10.5 sigma) an Halpha source that was 295% above the continuum just 0.083” from the star (edge of the inner 10 AU disk gap). We also detected (at 5 sigma) an excellent (though much fainter) ~1 Mjup mass Halpha planet candidate located auspiciously at the outer edge (145 AU) of the gap. If confirmed by our “second epoch” follow-up as common proper motion then this would be the lowest mass (~1 Mjup) planet ever imaged. SURVEY: Scaling off of this exciting success we propose to deeply image (120 min) all 14 nearby (D<250pc), bright (R<11 mag) , not edge-on (i<80 deg) , young (~5 Myr) transitional disks with MagAO simultaneously at Halpha and L’. In addition, we will use BrGamma instead of Halpha for 8 additional fainter (111 Mjup in mass, we integrate across our target list and find that, in the worst case of minimal masses (1+/-0.5 Mjup), and 3.4 mag extinction, at least seven ~1 Mjup planets should be discovered by this survey --meeting all three of our science goals above.

  7. Is High Temporal Resolution Achievable for Paediatric Cardiac Acquisitions during Several Heart Beats? Illustration with Cardiac Phase Contrast Cine-MRI

    PubMed Central

    Bonnemains, Laurent; Odille, Freddy; Meyer, Christophe; Hossu, Gabriella; Felblinger, Jacques; Vuissoz, Pierre-André

    2015-01-01

    Background During paediatric cardiac Cine-MRI, data acquired during cycles of different lengths must be combined. Most of the time, Feinstein’s model is used to project multiple cardiac cycles of variable lengths into a mean cycle. Objective To assess the effect of Feinstein projection on temporal resolution of Cine-MRI. Methods 1/The temporal errors during Feinstein’s projection were computed in 306 cardiac cycles fully characterized by tissue Doppler imaging with 6-phase analysis (from a population of 7 children and young adults). 2/The effects of these temporal errors on tissue velocities were assessed by simulating typical tissue phase mapping acquisitions and reconstructions. 3/Myocardial velocities curves, extracted from high-resolution phase-contrast cine images, were compared for the 6 volunteers with lowest and highest heart rate variability, within a population of 36 young adults. Results 1/The mean of temporal misalignments was 30 ms over the cardiac cycle but reached 60 ms during early diastole. 2/During phase contrast MRI simulation, early diastole velocity peaks were diminished by 6.1 cm/s leading to virtual disappearance of isovolumic relaxation peaks. 3/The smoothing and erasing of isovolumic relaxation peaks was confirmed on tissue phase mapping velocity curves, between subjects with low and high heart rate variability (p = 0.05). Conclusions Feinstein cardiac model creates temporal misalignments that impair high temporal resolution phase contrast cine imaging when beat-to-beat heart rate is changing. PMID:26599755

  8. Pioneering high angular resolution at GTC: FRIDA

    NASA Astrophysics Data System (ADS)

    Prieto, M. A.

    2017-03-01

    FRIDA imager and integral-field spectrograph will provide the GTC community with the first diffraction-limited angular resolutions of a 10 m telescope: 25 - 40 mas in the 1 - 2.5 um range. These angular resolutions are a factor 15 improvement with respect to those of current and/or planned instruments for GTC, factor 1.5 superior to that of JWST. In this talk I will develop on science paths for FRIDA, with natural and laser guide star that illustrate the potential and unique capabilities of GTCAO+FRIDA till the arrival of the ELTs.

  9. Improved localization accuracy in stochastic super-resolution fluorescence microscopy by K-factor image deshadowing.

    PubMed

    Ilovitsh, Tali; Meiri, Amihai; Ebeling, Carl G; Menon, Rajesh; Gerton, Jordan M; Jorgensen, Erik M; Zalevsky, Zeev

    2013-12-16

    Localization of a single fluorescent particle with sub-diffraction-limit accuracy is a key merit in localization microscopy. Existing methods such as photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) achieve localization accuracies of single emitters that can reach an order of magnitude lower than the conventional resolving capabilities of optical microscopy. However, these techniques require a sparse distribution of simultaneously activated fluorophores in the field of view, resulting in larger time needed for the construction of the full image. In this paper we present the use of a nonlinear image decomposition algorithm termed K-factor, which reduces an image into a nonlinear set of contrast-ordered decompositions whose joint product reassembles the original image. The K-factor technique, when implemented on raw data prior to localization, can improve the localization accuracy of standard existing methods, and also enable the localization of overlapping particles, allowing the use of increased fluorophore activation density, and thereby increased data collection speed. Numerical simulations of fluorescence data with random probe positions, and especially at high densities of activated fluorophores, demonstrate an improvement of up to 85% in the localization precision compared to single fitting techniques. Implementing the proposed concept on experimental data of cellular structures yielded a 37% improvement in resolution for the same super-resolution image acquisition time, and a decrease of 42% in the collection time of super-resolution data with the same resolution.

  10. Ultrahigh-resolution optical coherence tomography with monochromatic and chromatic aberration correction.

    PubMed

    Zawadzki, Robert J; Cense, Barry; Zhang, Yan; Choi, Stacey S; Miller, Donald T; Werner, John S

    2008-05-26

    We have developed an improved adaptive optics - optical coherence tomography (AO-OCT) system and evaluated its performance for in vivo imaging of normal and pathologic retina. The instrument provides unprecedented image quality at the retina with isotropic 3D resolution of 3.5 x 3.5 x 3.5 microm(3). Critical to the instrument's resolution is a customized achromatizing lens that corrects for the eye's longitudinal chromatic aberration and an ultra broadband light source (Delta lambda=112 nm lambda(0)= approximately 836 nm). The eye's transverse chromatic aberrations is modeled and predicted to be sufficiently small for the imaging conditions considered. The achromatizing lens was strategically placed at the light input of the AO-OCT sample arm. This location simplifies use of the achromatizing lens and allows straightforward implementation into existing OCT systems. Lateral resolution was achieved with an AO system that cascades two wavefront correctors, a large stroke bimorph deformable mirror (DM) and a micro-electromechanical system (MEMS) DM with a high number of actuators. This combination yielded diffraction-limited imaging in the eyes examined. An added benefit of the broadband light source is the reduction of speckle size in the axial dimension. Additionally, speckle contrast was reduced by averaging multiple B-scans of the same proximal patch of retina. The combination of improved micron-scale 3D resolution, and reduced speckle size and contrast were found to significantly improve visibility of microscopic structures in the retina.

  11. Pushing back the limits of Raman imaging by coupling super-resolution and chemometrics for aerosols characterization

    NASA Astrophysics Data System (ADS)

    Offroy, Marc; Moreau, Myriam; Sobanska, Sophie; Milanfar, Peyman; Duponchel, Ludovic

    2015-07-01

    The increasing interest in nanoscience in many research fields like physics, chemistry, and biology, including the environmental fate of the produced nano-objects, requires instrumental improvements to address the sub-micrometric analysis challenges. The originality of our approach is to use both the super-resolution concept and multivariate curve resolution (MCR-ALS) algorithm in confocal Raman imaging to surmount its instrumental limits and to characterize chemical components of atmospheric aerosols at the level of the individual particles. We demonstrate the possibility to go beyond the diffraction limit with this algorithmic approach. Indeed, the spatial resolution is improved by 65% to achieve 200 nm for the considered far-field spectrophotometer. A multivariate curve resolution method is then coupled with super-resolution in order to explore the heterogeneous structure of submicron particles for describing physical and chemical processes that may occur in the atmosphere. The proposed methodology provides new tools for sub-micron characterization of heterogeneous samples using far-field (i.e. conventional) Raman imaging spectrometer.

  12. Pushing back the limits of Raman imaging by coupling super-resolution and chemometrics for aerosols characterization

    PubMed Central

    Offroy, Marc; Moreau, Myriam; Sobanska, Sophie; Milanfar, Peyman; Duponchel, Ludovic

    2015-01-01

    The increasing interest in nanoscience in many research fields like physics, chemistry, and biology, including the environmental fate of the produced nano-objects, requires instrumental improvements to address the sub-micrometric analysis challenges. The originality of our approach is to use both the super-resolution concept and multivariate curve resolution (MCR-ALS) algorithm in confocal Raman imaging to surmount its instrumental limits and to characterize chemical components of atmospheric aerosols at the level of the individual particles. We demonstrate the possibility to go beyond the diffraction limit with this algorithmic approach. Indeed, the spatial resolution is improved by 65% to achieve 200 nm for the considered far-field spectrophotometer. A multivariate curve resolution method is then coupled with super-resolution in order to explore the heterogeneous structure of submicron particles for describing physical and chemical processes that may occur in the atmosphere. The proposed methodology provides new tools for sub-micron characterization of heterogeneous samples using far-field (i.e. conventional) Raman imaging spectrometer. PMID:26201867

  13. Modeling the Nucleus Laminaris of the Barn Owl: Achieving 20 ps Resolution on a 85-MHz-Clocked Digital Device

    PubMed Central

    Salomon, Ralf; Heinrich, Enrico; Joost, Ralf

    2012-01-01

    The nucleus laminaris of the barn owl auditory system is quite impressive, since its underlying time estimation is much better than the processing speed of the involved neurons. Since precise localization is also very important in many technical applications, this paper explores to what extent the main principles of the nucleus laminaris can be implemented in digital hardware. The first prototypical implementation yields a time resolution of about 20 ps, even though the chosen standard, low-cost device is clocked at only 85 MHz, which leads to an internal duty cycle of approximately 12 ns. In addition, this paper also explores the utility of an advanced sampling scheme, known as unfolding-in-time. It turns out that with this sampling method, the prototype can easily process input signals of up to 300 MHz, which is almost four times higher than the sampling rate. PMID:22347179

  14. Assessing resolution in super-resolution imaging.

    PubMed

    Demmerle, Justin; Wegel, Eva; Schermelleh, Lothar; Dobbie, Ian M

    2015-10-15

    Resolution is a central concept in all imaging fields, and particularly in optical microscopy, but it can be easily misinterpreted. The mathematical definition of optical resolution was codified by Abbe, and practically defined by the Rayleigh Criterion in the late 19th century. The limit of conventional resolution was also achieved in this period, and it was thought that fundamental constraints of physics prevented further increases in resolution. With the recent development of a range of super-resolution techniques, it is necessary to revisit the concept of optical resolution. Fundamental differences in super-resolution modalities mean that resolution is not a directly transferrable metric between techniques. This article considers the issues in resolution raised by these new technologies, and presents approaches for comparing resolution between different super-resolution methods.

  15. Achieving quasi-adiabatic thermal environment to maximize resolution power in very high-pressure liquid chromatography: Theory, models, and experiments.

    PubMed

    Gritti, Fabrice; Gilar, Martin; Jarrell, Joseph A

    2016-04-29

    A cylindrical vacuum chamber (inner diameter 5 cm) housing a narrow-bore 2.1 mm×100 mm column packed with 1.8 μm HSS-T3 fully porous particles was built in order to isolate thermally the chromatographic column from the external air environment. Consistent with statistical physics and the mean free path of air molecules, the experimental results show that natural air convection and conduction are fully eliminated for housing air pressures smaller than 10(-4) Torr. Heat radiation is minimized by wrapping up the column with low-emissivity aluminum-tape (emissivity coefficient ϵ=0.03 vs. 0.28 for polished stainless steel 316). Overall, the heat flux at the column wall is reduced by 96% with respect to standard still-air ovens. From a practical viewpoint, the efficiency of the column run at a flow rate of 0.6 mL/min at a constant 13,000 psi pressure drop (the viscous heat power is around 9 W/m) is improved by up to 35% irrespective of the analyte retention. Models of heat and mass transfer reveal that (1) the amplitude of the radial temperature gradient is significantly reduced from 0.30 to 0.01 K and (2) the observed improvement in resolution power stems from a more uniform distribution of the flow velocity across the column diameter. The eddy dispersion term in the van Deemter equation is reduced by 0.8±0.1 reduced plate height unit, a significant gain in column performance.

  16. Fabrication of two-color annular hybrid wave plate for three-dimensional super-resolution microscopy

    NASA Astrophysics Data System (ADS)

    Kumagai, Hiroshi; Iketaki, Yoshinori; Jahn, Kornel; Bokor, Nador

    2016-03-01

    In super-resolution microscopy, we use fluorescence depletion, where an erase beam quenches a molecule in the S1 state generated by a pump beam, and then prevents fluorescence from the S1 state. When a tight doughnut shaped erase beam with is focused on the dyed sample together with a Gaussian pump beam, the remaining fluorescence spot in the focal plane becomes smaller than the diffraction-limited size. Applying destructive interference to the erase beam, erase beam has a minute three-dimensional dark spot surrounded by the light near the focal region. Since this spot introduces fluorescence depletion along the optical axis as in the focal plane, we can achieve three-dimensional super-resolution microscopy. However, to overcome the diffraction limit, an extremely precise optical alignment is required for projecting the focused pump beam into the dark spot of the erase beam. To resolve this technical issue, we fabricated a two-color annular hybrid wave plate (TAHWP) by combining two multi-order wave quartz plates. Although the pump and erase beams co-axially pass through the plate; the pump beam retains its original Gaussian shape, while the erase beam undergoes destructive interference. Inserting the TAHWP into a commercial scanning laser microscope, a three-dimensional spherical fluorescence spot with a volume of (~100 nm)3 can be created. Beside eliminating alignment problems and yielding a compact setup, the TAHWP makes our proposed method very suitable for commercial microscope systems. In this study, we report about detailed fabrication procedure and three-dimensional image properties given by the TAHWP.

  17. High resolution imaging with multilayer telescopes: resolution performance of the MSSTA II telescopes

    SciTech Connect

    Martinez-Galarce, Dennis S.; Walker, Arthur B. C. II; Gore, David B.; Kankelborg, Charles C.; Hoover, Richard B.; Barbee, T. W. Jr.; Boerner, P. F. X.

    2000-04-01

    The Multi-Spectral Solar Telescope Array (MSSTA) is a sounding rocket-borne observatory composed of a set of normal-incidence multilayer-coated telescopes that obtained selected bandpass spectroheliograms (44 to 1550 Aa) of the solar atmosphere. These spectroheliograms were recorded on specially fabricated XUV and FUV 70-mm Kodak film. Rocket launches of this instrument payload took place in 1991 (MSSTA I) and 1994 (MSSTA II) at the White Sands Missile Test Range in New Mexico, sponsored by the National Aeronautics and Space Administration (NASA) sounding rocket experiment program. Immediately prior to the 1994 launch, visible light focusing tests of each telescope were performed in situ using a 1951 standard Air Force high-resolution test target, to measure optical resolution performance. We determined that the MSSTA II telescopes performed at diffraction-limited resolutions down to 0.70 arcsec at visible wavelengths. Based on these measurements, we calculate an upper bound to the focusing errors that incorporate the sum of all uncorrelated system focus errors that affect resolution performance. Coupling these upper bound estimates with the in-band diffraction limits, surface scattering errors and payload pointing jitter, we demonstrate that 11 of 19 MSSTA II telescopes--having negligible figures of focus errors in comparison to the corresponding visible diffraction limits--performed at sub arcsecond resolution at their operational FUV/EUV/XUV wavelengths during flight. We estimate the in-band performance down to 0.14{+-}0.08 arcsec. (c) 2000 Society of Photo-Optical Instrumentation Engineers.

  18. Performance of the WIYN high-resolution infrared camera

    NASA Astrophysics Data System (ADS)

    Meixner, Margaret; Smee, Stephen; Doering, Ryan L.; Barkhouser, Robert H.; Miller, Todd; Orndorff, Joseph; Knezek, Patricia; Churchwell, Edward; Scharfstein, Gregg; Percival, Jeffrey; Mills, David; Corson, Charles; Joyce, Richard; Ferguson, Brian; Otsuka, Masaaki

    2010-07-01

    The WIYN High Resolution Infrared Camera (WHIRC) has been a general-use instrument at the WIYN telescope on Kitt Peak since 2008. WHIRC is a near-infrared (0.8 - 2.5 μm) camera with a filter complement of J, H, Ks broadband and 10 narrowband filters, utilizing a 2048 × 2048 HgCdTe array from Raytheon's VIRGO line, developed for the VISTA project. The compact on-axis refractive optical design makes WHIRC the smallest near-IR camera with this capability. WHIRC is installed on the WIYN Tip-Tilt Module (WTTM) port and can achieve near diffraction-limited imaging with a FWHM of ~0.25 arcsec at Ks with active WTTM correction and routinely delivers ~0.6 arcsec FWHM images without WTTM correction. During its first year of general use operation at WIYN, WHIRC has been used for high definition near-infrared imaging studies of a wide range of astronomical phenomena including star formation regions, stellar populations and interstellar medium in nearby galaxies, high-z galaxies and transient phenomena. We discuss performance and data reduction issues such as distortion, pupil ghost, and fringe removal and the development of new tools for the observing community such as an exposure time calculator and data reduction pipeline.

  19. High Resolution Imaging of Space Objects.

    DTIC Science & Technology

    1981-03-01

    one second of arc, com - pared with 0.02 seconds of arc, the theoretical diffraction-limited resolution of a five-meter diameter telescope. That is...follows: First, fn = f *f(0) D (3, i,, so that factor can be divided out from the last three terms ot Lq. Vk (A). Second, let the coefficients of...tnor " porno one of them yields a function G(w) sucn that, Uy C-orol ay G) U, U> JC daf q are not equivalent. I i#: By Lemma , if F a, n n nlY one non

  20. Super-resolution photoacoustic imaging of single gold nanoparticles

    NASA Astrophysics Data System (ADS)

    Lee, Seunghyun; Kwon, Owoong; Jeon, Mansik; Song, Jaejung; Jo, Minguk; Kim, Sungjee; Son, Junwoo; Kim, Yunseok; Kim, Chulhong

    2016-03-01

    Photoacoustic imaging (PAI) is an emerging hybrid imaging modality that can provide a strong optical absorption contrast using the photoacoustic (PA) effect, and breaks through the fundamental imaging depth limit of existing optical microscopy such as optical coherence tomography (OCT), confocal or two-photon microscopy. In PAI, a short-pulsed laser is illuminated to the tissue, and the PA waves are generated by thermoelastic expansion. Despite the high lateral resolution of optical-resolution photoacoustic microscopy (OR-PAM) thanks to the tight optical focus, the lateral resolution of OR-PAM is limited to the optical diffraction limit, which is approximately a half of the excitation wavelength. Here, we demonstrate a new super-resolution photoacoustic microscopy (SR-PAM) system by breaking the optical diffraction limit. The conventional microscopes with nanoscale resolutions such as a scanning electron microscope (SEM) and transmission electron microscope (TEM) are typically used to image the structures of nanomaterials, but these systems should work in a high vacuum environment and cannot provide the optical properties of the materials. Our newly developed SR-PAM system provides the optical properties with a nanoscale resolution in a normal atmosphere. We have photoacoustically imaged single gold nanoparticles with an average size of 80 nm in diameter and shown their PA expansion properties individually. The lateral resolution of this system was approximately 20 nm. Therefore, this tool will provide an unprecedented optical absorption property with an accurate nanoscale resolution and greatly impact on materials science and nanotechnology field.

  1. Large area super-resolution chemical imaging via rapid dithering of a nanoprobe

    NASA Astrophysics Data System (ADS)

    Languirand, Eric R.; Cullum, Brian M.

    2015-05-01

    Super-resolution chemical imaging via Raman spectroscopy provides a significant ability to simultaneously or pseudosimultaneously monitor numerous label-free analytes while elucidating their spatial distribution on the surface of the sample. However, spontaneous Raman is an inherently weak phenomenon making trace detection and thus superresolution imaging extremely difficult, if not impossible. To circumvent this and allow for trace detection of the few chemical species present in any sub-diffraction limited resolution element of an image, we have developed a surface enhanced Raman scattering (SERS) coherent fiber-optic imaging bundle probe consisting of 30,000 individual fiber elements. When the probes are tapered, etched and coated with metal, they provide circular Raman chemical images of a sample with a field of view of approximately 20μm (i.e. diameter) via the array of 30,000 individual 50 nm fiber elements. An acousto-optic tunable filter is used to rapidly scan or select discrete frequencies for multi- or hyperspectral analysis. Although the 50nm fiber element dimensions of this probe inherently provide spatial resolutions of approximately 100nm, further increases in the spatial resolution can be achieved by using a rapid dithering process. Using this process, additional images are obtained one-half fiber diameter translations in the x- and y- planes. A piezostage drives the movement, providing the accurate and reproducible shifts required for dithering. Optimal probability algorithms are then used to deconvolute the related images producing a final image with a three-fold increase in spatial resolution. This paper describes super-resolution chemical imaging using these probes and the dithering method as well as its potential applications in label-free imaging of lipid rafts and other applications within biology and forensics.

  2. A Comprehensive Approach to High-Resolution Daylight Imaging for SSA

    NASA Astrophysics Data System (ADS)

    Hart, M.; Jefferies, S.; Hope, D.; Nagy, J.; Swindle, R.

    2016-09-01

    High resolution daytime imaging of resident space objects (RSO) from the ground is presently severely challenging. At visible wavelengths, where diffraction-limited resolution is the highest before the atmosphere becomes opaque in the UV, shot noise from the bright background degrades the information that may be recovered from RSO imagery. Total exposure times must be limited in order to avoid motion blur induced either by the object's intrinsic rotation or simply by its orbital motion over the site. Fundamentally, then, one cannot collect enough light from the object to achieve adequate signal-to-noise ratio (SNR) in the presence of very high noise before the apparent shape of the object has changed. To overcome this limitation, we propose in this paper a suite of techniques which we believe will collectively enable high-resolution imaging during daylight. The approach, which has yet to be fully implemented, relies on a sequence of short-exposure images from a high-cadence camera together with simultaneous wave-front sensor (WFS) measurements acquired from a filtered sodium laser guide star. We then directly estimate the three-dimensional shape of the RSO using a formalism similar to the concept of deconvolution from wave-front sensing (DWFS). In this way, provided that the intrinsic shape of the RSO does not significantly change during the course of the observations, we can combine data from quite different pose angles in order to achieve a high resolution result with adequate SNR. By adopting this approach, we expect an improvement of 3-4 stellar magnitudes in the faintest satellites that may be characterized independent of the telescope and observing waveband. Furthermore, a model derived from observations by one sensor may be used as the basis for the restoration of data sets from widely disparate telescopes and sensor modalities; data fusion in this sense is a natural feature of the approach.

  3. A novel long-wave infrared high resolution continuous zoom lens with uncooled thermal detector

    NASA Astrophysics Data System (ADS)

    Bao, Jiaqi; Yu, Kan; Ji, Zijuan

    2016-09-01

    Infrared imaging lens is one of the key components of a video security camera. A novel long-wave infrared continuous zoom lens is developed based on the 640×512 high resolution uncooled infrared thermal detector which can substitute the high cost cooled infrared detector. The zoom lens contains five germanium lens and one chalcogenide glass lens, which working in the wavelength range of 8 12 μm. Its F number range is in 1 1.1 while the focus length is changing from 20 to 120 mm. Based on the zoom lens design theory, the positive lens mechanical compensation structure is used to calculate the optical parameters and optimize the cam zoom curve, which can have a smooth continuous zoom in the range of all focus lengths. The image analysis show that the system has achieved the modulation transfer function (MTF) value above 0.45 which spatial frequency is 30 lp/mm. The spot diagrams RMS radius is less than 6.3μm which is near the diffraction limit. The real test photos indicate that the lens has the advantages of high resolution, large aperture, smooth zoom and stable image plane. Due to the high image quality and low cost, the continuous zoom lens is easily to be fabricated.

  4. Super-resolution microscopy of mitochondria.

    PubMed

    Jakobs, Stefan; Wurm, Christian A

    2014-06-01

    Mitochondria, the powerhouses of the cell, are essential organelles in eukaryotic cells. With their complex inner architecture featuring a smooth outer and a highly convoluted inner membrane, they are challenging objects for microscopy. The diameter of mitochondria is generally close to the resolution limit of conventional light microscopy, rendering diffraction-unlimited super-resolution light microscopy (nanoscopy) for imaging submitochondrial protein distributions often mandatory. In this review, we discuss what can be expected when imaging mitochondria with conventional diffraction-limited and diffraction-unlimited microscopy. We provide an overview on recent studies using super-resolution microscopy to investigate mitochondria and discuss further developments and challenges in mitochondrial biology that might by addressed with these technologies in the future.

  5. High Resolution Imaging of Io's Volcanoes with LBTI

    NASA Astrophysics Data System (ADS)

    Conrad, Al; Leisenring, Jarron; de Kleer, Katherine; Skemer, Andy; Hinz, Philip; Skrutskie, Michael; Veillet, Christian; de Pater, Imke; Bertero, Mario; Boccacci, Patrizia; Defrère, Denis; Hofmann, Karl-Heinz; La Camera, Andrea; Schertl, Dieter; Spencer, John; Weigelt, Gerd; Woodward, Charles E.

    2014-11-01

    The Large Binocular Telescope (LBT), located on Mount Graham in eastern Arizona, employs two 8.4 meter mirrors with a 14.4 center-to-center separation on a common mount. Coherent combination of these two AO-corrected apertures via the LBT Interferometer (LBTI) produces Fizeau interferometric images with spatial resolution consistent with the diffraction limit of the 22.8-meter aperture. In particular LBTI resolves thermal signatures (i.e., features observed at M-band) on the surface of Io down to ~150 kilometers; a two-fold improvement over what has previously been possible from the ground. We show images collected with LBTI on December 24, 2013, in which Loki's shape is clearly resolved and at least fourteen additional volcanic hot spots are detected.We analyze three locations in the LBTI data: emission features within Loki Patera, the area near Rarog and Heno Patarae, and a hot spot seen in the Colchis Regio.For Loki Patera, we interpret spatially resolved variation in the emission within that region. With M-band resolution that is comparable to what has previously been achievable only at K-band, we compare localized emission features with what has been seen in earlier observations at shorter wavelengths.Thermal emission from activity at Rarog and Heno Patarae is well resolved in these images, while a third hot-spot in the nearby Lerna Regio is also clearly resolved. This area is of special interest since it was the site of two high-effusion outbursts on August 15th, 2013 [de Pater et al. (2014) Icarus].Lastly, we explore a hot-spot seen in the Colchis Regio that may be a remnant of a violent outburst detected on August 29th, 2013 [de Kleer et al. (2014) Icarus].

  6. Performance of the WIYN high-resolution infrared camera

    NASA Astrophysics Data System (ADS)

    Meixner, Margaret; Smee, Stephen; Doering, Ryan L.; Barkhouser, Robert H.; Miller, Todd; Orndoff, Joseph; Knezek, Patricia; Churchwell, Edward; Scharfstein, Gregg; Percival, Jeff; Mills, David; Corson, Charles

    2008-07-01

    We present the design overview and on-telescope performance of the WIYN High Resolution Infrared Camera (WHIRC). As a dedicated near-infrared (0.8-2.5 μm) camera on the WIYN Tip-Tilt Module (WTTM), WHIRC will provide near diffraction-limited imaging with a typical FWHM of ~0.25". WHIRC uses a 2048 x 2048 HgCdTe array from Raytheon's VIRGO line, which is a spinoff from the VISTA project. The WHIRC filter complement includes J, H KS, and 10 narrowband filters. WHIRC's compact design makes it the smallest near-IR camera with this capability. We determine a gain of 3.8 electrons ADU-1 via a photon transfer analysis and a readout noise of ~27 electrons. A measured dark current of 0.23 electrons s-1 indicates that the cryostat is extremely light tight. A plate scale of 0.098" pixel-1 results in a field of view (FOV) of ~3' x 3', which is a compromise between the highest angular resolution achievable and the largest FOV correctable by WTTM. Measured throughput values (~0.33 in H-band) are consistent with those predicted for WHIRC based on an elemental analysis. WHIRC was delivered to WIYN in July 2007 and was opened for shared risk use in Spring 2008. WHIRC will be a facility instrument at the WIYN telescope enabling high definition near-infrared imaging studies of a wide range of astronomical phenomena including star formation regions, proto-planetary disks, stellar populations and interstellar medium in nearby galaxies, and supernova and gamma-ray burst searches.

  7. Three-dimensional optical-resolution photoacoustic microscopy.

    PubMed

    Hu, Song; Maslov, Konstantin; Wang, Lihong V

    2011-05-03

    Optical microscopy, providing valuable insights at the cellular and organelle levels, has been widely recognized as an enabling biomedical technology. As the mainstays of in vivo three-dimensional (3-D) optical microscopy, single-/multi-photon fluorescence microscopy and optical coherence tomography (OCT) have demonstrated their extraordinary sensitivities to fluorescence and optical scattering contrasts, respectively. However, the optical absorption contrast of biological tissues, which encodes essential physiological/pathological information, has not yet been assessable. The emergence of biomedical photoacoustics has led to a new branch of optical microscopy optical-resolution photoacoustic microscopy (OR-PAM), where the optical irradiation is focused to the diffraction limit to achieve cellular or even subcellular level lateral resolution. As a valuable complement to existing optical microscopy technologies, OR-PAM brings in at least two novelties. First and most importantly, OR-PAM detects optical absorption contrasts with extraordinary sensitivity (i.e., 100%). Combining OR-PAM with fluorescence microscopy or with optical-scattering-based OCT (or with both) provides comprehensive optical properties of biological tissues. Second, OR-PAM encodes optical absorption into acoustic waves, in contrast to the pure optical processes in fluorescence microscopy and OCT, and provides background-free detection. The acoustic detection in OR-PAM mitigates the impacts of optical scattering on signal degradation and naturally eliminates possible interferences (i.e., crosstalks) between excitation and detection, which is a common problem in fluorescence microscopy due to the overlap between the excitation and fluorescence spectra. Unique for optical absorption imaging, OR-PAM has demonstrated broad biomedical applications since its invention, including, but not limited to, neurology, ophthalmology, vascular biology, and dermatology. In this video, we teach the system

  8. Super-resolution optical microscopy by using dielectric microwires

    NASA Astrophysics Data System (ADS)

    Darafsheh, Arash; Wu, Gaoxiang; Yang, Shu; Finlay, Jarod C.

    2016-03-01

    We demonstrate that super-resolution imaging of specimens containing sub-diffraction-limited features is feasible by using dielectric microwires fabricated through capillary force lithography followed by photopatterning. As supplementary micron scale cylindrical lenses, we fabricated uniform-sized microwires with and 5 and 10 μm diameters and refractive index ~1.3-1.6. The microwires are placed in contact with the specimen to collect the information of the sub-wavelength features of the specimen and transmit them to the far-field with magnification enabling imaging with two-fold resolution improvement. Potential applications of our imaging technique include biological imaging, microfluidics, and nanophotonics applications.

  9. A general strategy for developing cell-permeable photo-modulatable organic fluorescent probes for live-cell super-resolution imaging

    PubMed Central

    Pan, Deng; Hu, Zhe; Qiu, Fengwu; Huang, Zhen-Li; Ma, Yilong; Wang, Yina; Qin, Lingsong; Zhang, Zhihong; Zeng, Shaoqun; Zhang, Yu-Hui

    2014-01-01

    Single-molecule localization microscopy (SMLM) achieves super-resolution imaging beyond the diffraction limit but critically relies on the use of photo-modulatable fluorescent probes. Here we report a general strategy for constructing cell-permeable photo-modulatable organic fluorescent probes for live-cell SMLM by exploiting the remarkable cytosolic delivery ability of a cell-penetrating peptide (rR)3R2. We develop photo-modulatable organic fluorescent probes consisting of a (rR)3R2 peptide coupled to a cell-impermeable organic fluorophore and a recognition unit. Our results indicate that these organic probes are not only cell permeable but can also specifically and directly label endogenous targeted proteins. Using the probes, we obtain super-resolution images of lysosomes and endogenous F-actin under physiological conditions. We resolve the dynamics of F-actin with 10 s temporal resolution in live cells and discern fine F-actin structures with diameters of ~80 nm. These results open up new avenues in the design of fluorescent probes for live-cell super-resolution imaging. PMID:25410769

  10. Subwavelength resolution from multilayered structure (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Cheng, Bo Han; Jen, Yi-Jun; Liu, Wei-Chih; Lin, Shan-wen; Lan, Yung-Chiang; Tsai, Din Ping

    2016-10-01

    Breaking optical diffraction limit is one of the most important issues needed to be overcome for the demand of high-density optoelectronic components. Here, a multilayered structure which consists of alternating semiconductor and dielectric layers for breaking optical diffraction limitation at THz frequency region are proposed and analyzed. We numerically demonstrate that such multilayered structure not only can act as a hyperbolic metamaterial but also a birefringence material via the control of the external temperature (or magnetic field). A practical approach is provided to control all the diffraction signals toward a specific direction by using transfer matrix method and effective medium theory. Numerical calculations and computer simulation (based on finite element method, FEM) are carried out, which agree well with each other. The temperature (or magnetic field) parameter can be tuned to create an effective material with nearly flat isofrequency feature to transfer (project) all the k-space signals excited from the object to be resolved to the image plane. Furthermore, this multilayered structure can resolve subwavelength structures at various incident THz light sources simultaneously. In addition, the resolution power for a fixed operating frequency also can be tuned by only changing the magnitude of external magnetic field. Such a device provides a practical route for multi-functional material, photolithography and real-time super-resolution image.

  11. High-resolution imaging with AEOS

    NASA Astrophysics Data System (ADS)

    Patience, Jennifer; Macintosh, Bruce A.; Max, Claire E.

    2001-12-01

    The U.S. Air Force Advanced Electro-Optical System (AEOS) which includes a 941 actuator adaptive optics system on a 3.7 m telescope has recently been made available for astronomical programs. Operating at a wavelength of 750 nm, the diffraction-limited angular resolution of the system is 0'.04; currently, the magnitude limit is V approximately 7 mag. At the distances of nearby open clusters, diffraction- limited images should resolve companions with separations as small as 4 - 6 AU - comparable to the Sun-Jupiter distance. The ability to study such close separations is critical, since most companions are expected to have separations in the few AU to tens of AU range. With the exceptional angular resolution of the current AEOS setup, but restricted target magnitude range, we are conducting a companion search of a large, well-defined sample of bright early-type stars in nearby open clusters and in the field. Our data set will both characterize this relatively new adaptive optics system and answer questions in binary star formation and stellar X- ray activity. We will discuss our experience using AEOS, the data analysis involved, and our initial results.

  12. Fluorescent probes for super-resolution imaging in living cells.

    PubMed

    Fernández-Suárez, Marta; Ting, Alice Y

    2008-12-01

    In 1873, Ernst Abbe discovered that features closer than approximately 200 nm cannot be resolved by lens-based light microscopy. In recent years, however, several new far-field super-resolution imaging techniques have broken this diffraction limit, producing, for example, video-rate movies of synaptic vesicles in living neurons with 62 nm spatial resolution. Current research is focused on further improving spatial resolution in an effort to reach the goal of video-rate imaging of live cells with molecular (1-5 nm) resolution. Here, we describe the contributions of fluorescent probes to far-field super-resolution imaging, focusing on fluorescent proteins and organic small-molecule fluorophores. We describe the features of existing super-resolution fluorophores and highlight areas of importance for future research and development.

  13. Translation Microscopy (TRAM) for super-resolution imaging

    PubMed Central

    Qiu, Zhen; Wilson, Rhodri S; Liu, Yuewei; R Dun, Alison; Saleeb, Rebecca S; Liu, Dongsheng; Rickman, Colin; Frame, Margaret; Duncan, Rory R; Lu, Weiping

    2016-01-01

    Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the requirement for bespoke sample preparation. We present a novel, simple and multi-color super-resolution microscopy technique, called translation microscopy (TRAM), in which a super-resolution image is restored from multiple diffraction-limited resolution observations using a conventional microscope whilst translating the sample in the image plane. TRAM can be implemented using any microscope, delivering up to 7-fold resolution improvement. We compare TRAM with other super-resolution imaging modalities, including gated stimulated emission deletion (gSTED) microscopy and atomic force microscopy (AFM). We further developed novel ‘ground-truth’ DNA origami nano-structures to characterize TRAM, as well as applying it to a multi-color dye-stained cellular sample to demonstrate its fidelity, ease of use and utility for cell biology. PMID:26822455

  14. Resolution of sleepiness and fatigue: a comparison of bupropion and selective serotonin reuptake inhibitors in subjects with major depressive disorder achieving remission at doses approved in the European Union.

    PubMed

    Cooper, James A; Tucker, Vivian L; Papakostas, George I

    2014-02-01

    Unlike selective serotonin reuptake inhibitors (SSRIs), bupropion may be classified as a dual noradrenaline and dopamine reuptake inhibitor, a difference with potential implications for the treatment of residual sleepiness and fatigue in major depressive disorder (MDD). Post-hoc analysis of subjects with remitted MDD was performed on data pooled from six double-blind, randomized trials comparing the European Union (EU)-approved dose of ≤300 mg/day bupropion with SSRIs (sertraline, paroxetine or escitalopram) for the resolution of sleepiness and fatigue. Hypersomnia score was defined as the sum of scores of the Hamilton Depression Rating Scale (HDRS) items 22, 23, and 24; fatigue score as HDRS item 13 score; and remission as HDRS-17≤7. Similar proportions of bupropion- and SSRI-treated subjects achieved remission at study endpoint (169/343, 49.3% vs 324/656, 49.4%; last observation carried forward (LOCF), p=0.45). Fewer bupropion-treated remitters had residual symptoms of sleepiness (32/169, 18.9% vs 104/324, 32.1%; p<0.01) and fatigue (33/169, 19.5% vs 98/324, 30.2%; p<0.05). Bupropion-treated remitters also showed greater improvement (mean change from baseline) in sleepiness (p<0.05) and fatigue scores (p<0.01) at endpoint: benefits were evident from week 2 for sleepiness (p<0.01) and week 4 for fatigue (p<0.01). Bupropion treatment at the EU-approved dose of ≤300 mg/day may offer advantages over SSRIs in the resolution of sleepiness and fatigue in remitted MDD patients.

  15. Illustration of the use of multimode deformable plane mirrors to record high-resolution concave gratings: results for the Cosmic Origins Spectrograph gratings of the Hubble Space Telescope.

    PubMed

    Duban, M; Dohlen, K; Lemaitre, G R

    1998-11-01

    To illustrate the efficiency of using a deformable plane mirror to record holographic gratings, we have computed the three gratings for the Cosmic Origins Spectrograph. Their working conditions are severe, since they have to correct the residual spherical aberration of the Hubble Space Telescope. Nevertheless, all images obtained are largely diffraction limited with regard to the resolution.

  16. Focal plane actuation for the development of a high resolution suborbital telescope

    NASA Astrophysics Data System (ADS)

    Duke Miller, Alex; Scowen, Paul A.; Veach, Todd

    2016-01-01

    We present a hexapod stabilized focal plane as the key instrument for a proposed suborbital balloon mission. Balloon gondolas currently achieve 1-2 arcsecond pointing error, but cannot correct for unavoidable jitter movements (~50μm at 20hz) caused by wind rushing over balloon surfaces, thermal variations, cryocoolers, and reaction wheels. The jitter causes image blur during exposures and is the limiting resolution of the system. To solve this, the hexapod system actuates the focal plane to counteract the jitter through real-time closed loop feedback from star-trackers. Removal of this final jitter term decreases pointing error by an order of magnitude and allows for true diffraction-limited observation. This boost in resolution will allow for Hubble-quality imaging for a fraction of the cost. Tip-tilt pointing systems have been used for these purposes in the past, but require additional optics and introduce multiple reflections. The hexapod system, rather, is compact and can be plugged into the focal point of nearly any configuration. The design also thermally isolates the hexapod from the cryogenic focal plane enabling the use of well-established non-cryogenic hexapod technology. High-resolution time domain multispectral imaging of the gas giant outer planets, especially in the UV range, is of particular interest to the planetary community, and a suborbital telescope with the hexapod stabilization in place would provide a wealth of new data. On an Antarctic ~100-day Long-Duration-Balloon mission the continued high-resolution imaging of gas giant storm systems would provide cloud formation and evolution data second to only a Flagship orbiter.

  17. Recent advances in super-resolution fluorescence imaging and its applications in biology.

    PubMed

    Han, Rongcheng; Li, Zhenghong; Fan, Yanyan; Jiang, Yuqiang

    2013-12-20

    Fluorescence microscopy has become an essential tool for biological research because it can be minimally invasive, acquire data rapidly, and target molecules of interest with specific labeling strategies. However, the diffraction-limited spatial resolution, which is classically limited to about 200 nm in the lateral direction and about 500 nm in the axial direction, hampers its application to identify delicate details of subcellular structure. Extensive efforts have been made to break diffraction limit for obtaining high-resolution imaging of a biological specimen. Various methods capable of obtaining super-resolution images with a resolution of tens of nanometers are currently available. These super-resolution techniques can be generally divided into three primary classes: (1) patterned illumination-based super-resolution imaging, which employs spatially and temporally modulated illumination light to reconstruct sub-diffraction structures; (2) single-molecule localization-based super-resolution imaging, which localizes the profile center of each individual fluorophore at subdiffraction precision; (3) bleaching/blinking-based super-resolution imaging. These super-resolution techniques have been utilized in different biological fields and provide novel insights into several new aspects of life science. Given unique technical merits and commercial availability of super-resolution fluorescence microscope, increasing applications of this powerful technique in life science can be expected.

  18. A simple, high efficiency, high resolution spectropolarimeter

    NASA Astrophysics Data System (ADS)

    Barden, Samuel C.

    2012-09-01

    A simple concept is described that uses volume phase holographic gratings as polarizing dispersers for a high efficiency, high resolution spectropolarimeter. Although the idea has previously been mentioned in the literature as possible, such a concept has not been explored in detail. Performance analysis is presented for a VPHG spectropolarimeter concept that could be utilized for both solar and night-time astronomy. Instrumental peak efficiency can approach 100% with spectral dispersions permitting R~200,000 spectral resolution with diffraction limited telescopes. The instrument has 3-channels: two dispersed image planes with orthogonal polarization and an undispersed image plane. The concept has a range of versatility where it could be configured (with appropriate half-wave plates) for slit-fed spectroscopy or without slits for snapshot/hyperspectral/tomographic spectroscopic imaging. Multiplex gratings could also be used for the simultaneous recording of two separate spectral bands or multiple instruments could be daisy chained with beam splitters for further spectral coverage.

  19. Super-resolution microscopy: a comparative treatment.

    PubMed

    Kasuboski, James M; Sigal, Yury J; Joens, Matthew S; Lillemeier, Bjorn F; Fitzpatrick, James A J

    2012-10-01

    One of the fundamental limitations of optical microscopy is that of diffraction, or in essence, how small a beam of light can be focused by using an optical lens system. This constraint, or barrier if you will, was theoretically described by Ernst Abbe in 1873 and is roughly equal to half the wavelength of light used to probe the system. Many structures, particularly those within cells, are much smaller than this limit and thus are difficult to visualize. Over the last two decades, a new field of super-resolution imaging has been created and been developed into a broad range of techniques that allow routine imaging beyond the far-field diffraction limit of light. In this unit we outline the basic principles of the various super-resolution imaging modalities, paying particular attention to the technical considerations for biological imaging. Furthermore, we discuss their various applications in the imaging of both fixed and live biological samples.

  20. Endlessly single-mode photonic crystal fiber as a high resolution probe.

    PubMed

    Valtna-Lukner, Heli; Repän, Jaagup; Valdma, Sandhra-Mirella; Piksarv, Peeter

    2016-11-20

    We sample ultra-broadband light, focused onto a diffraction-limited spot, to an endlessly single-mode photonic crystal fiber (ESM) and detect both the field amplitude and phase using a SEA TADPOLE interferometer. We resolve spatial features up to 2.5 times finer than the fiber mode size while sampling the periodic features of the bipolar oscillating field in the transverse section. The resolution enhancement is expected also in other types of single-mode fibers in intensity measurements and leads to an inexpensive method for characterizing the point-spread function of such optical fields, e.g., diffraction-limited spots from microscope objectives. In addition, we demonstrate the guidance of a high-NA light field in the fine structure of an ESM fiber mode. The results are especially valuable for devices where a fiber tip acts as an input slit and defines the spatial resolution, e.g., fiber-based interferometers, spectrometers, and sensors.

  1. Development of a measurement technique for ion distribution in an extended nanochannel by super-resolution-laser-induced fluorescence.

    PubMed

    Kazoe, Yutaka; Mawatari, Kazuma; Sugii, Yasuhiko; Kitamori, Takehiko

    2011-11-01

    Ion behavior confined in extended nanospace (10(1)-10(3) nm) is important for nanofluidics and nanochemistry with dominant surface effects. In this paper, we developed a new measurement technique of ion distribution in the nanochannel by super-resolution-laser-induced fluorescence. Stimulated emission depletion microscopy was used to achieve a spatial resolution of 87 nm higher than the diffraction limit. Fluorescein was used for ratiometric measurement of pH with two excitation wavelengths. The pH profile in a 2D nanochannel of 410 nm width and 405 nm depth was successfully measured at an uncertainty of 0.05. The excess protons, showing lower pH than the bulk, nonuniformly distributed in the nanochannel to cancel the negative charge of glass wall, especially when the electric double layer is thick compared to the channel size. The present study first revealed the ion distribution near the surface or in the nanochannel, which is directly related to the electric double layer. In addition, the obtained proton distribution is important to understand the nanoscale water structure between single molecules and continuum phase. This technique will greatly contribute to understanding the basic science in nanoscale and interfacial dynamics, which are strongly required to develop novel miniaturized systems for biochemical analysis and further applications.

  2. First Light Adaptive Optics Images from the Keck II Telescope: A New Era of High Angular Resolution Imagery

    NASA Astrophysics Data System (ADS)

    Wizinowich, P.; Acton, D. S.; Shelton, C.; Stomski, P.; Gathright, J.; Ho, K.; Lupton, W.; Tsubota, K.; Lai, O.; Max, C.; Brase, J.; An, J.; Avicola, K.; Olivier, S.; Gavel, D.; Macintosh, B.; Ghez, A.; Larkin, J.

    2000-03-01

    Adaptive optics (AO) is a technology that corrects in real time for the blurring effects of atmospheric turbulence, in principle allowing Earth-bound telescopes to achieve their diffraction limit and to ``see'' as clearly as if they were in space. The power of AO using natural guide stars has been amply demonstrated in recent years on telescopes up to 3-4 m in diameter. The next breakthrough in astronomical resolution was expected to occur with the implementation of AO on the new generation of large, 8-10 m diameter telescopes. In this paper we report the initial results from the first of these AO systems, now coming on line on the 10 m diameter Keck II Telescope. The results include the highest angular resolution images ever obtained from a single telescope (0.022" and 0.040" at 0.85 and 1.65 μm wavelengths, respectively), as well as tests of system performance on three astronomical targets.

  3. Note on the classification of super-resolution in far-field microscopy and information theory

    NASA Astrophysics Data System (ADS)

    Passon, Oliver; Grebe-Ellis, Johannes

    2016-07-01

    In recent years several far-field microscopy techniques have been developed which manage to overcome the diffraction limit of resolution. A unifying classification scheme for them is clearly desirable. We argue that existing schemes based on the information capacity of the optical system can not easily be extended to cover e.g., STED microscopy or techniques based on single molecule imaging. We suggest a classification based on a reconstruction of the Abbe limit.

  4. Note on the classification of super-resolution in far-field microscopy and information theory.

    PubMed

    Passon, Oliver; Grebe-Ellis, Johannes

    2016-07-01

    In recent years, several far-field microscopy techniques have been developed which manage to overcome the diffraction limit of resolution. A unifying classification scheme for them is clearly desirable. We argue that existing schemes based on the information capacity of the optical system cannot easily be extended to cover, e.g., stimulated emission depletion microscopy or techniques based on single-molecule imaging. We suggest a classification based on a reconstruction of the Abbe limit.

  5. High-resolution adaptive optics scanning laser ophthalmoscope with dual deformable mirrors for large aberration correction

    SciTech Connect

    Chen, D; Jones, S M; Silva, D A; Olivier, S S

    2007-01-25

    Scanning laser ophthalmoscopes with adaptive optics (AOSLO) have been shown previously to provide a noninvasive, cellular-scale view of the living human retina. However, the clinical utility of these systems has been limited by the available deformable mirror technology. In this paper, we demonstrate that the use of dual deformable mirrors can effectively compensate large aberrations in the human retina, making the AOSLO system a viable, non-invasive, high-resolution imaging tool for clinical diagnostics. We used a bimorph deformable mirror to correct low-order aberrations with relatively large amplitudes. The bimorph mirror is manufactured by Aoptix, Inc. with 37 elements and 18 {micro}m stroke in a 10 mm aperture. We used a MEMS deformable mirror to correct high-order aberrations with lower amplitudes. The MEMS mirror is manufactured by Boston Micromachine, Inc with 144 elements and 1.5 {micro}m stroke in a 3 mm aperture. We have achieved near diffraction-limited retina images using the dual deformable mirrors to correct large aberrations up to {+-} 3D of defocus and {+-} 3D of cylindrical aberrations with test subjects. This increases the range of spectacle corrections by the AO systems by a factor of 10, which is crucial for use in the clinical environment. This ability for large phase compensation can eliminate accurate refractive error fitting for the patients, which greatly improves the system ease of use and efficiency in the clinical environment.

  6. High resolution imaging system for Udaipur Solar Observatory

    NASA Astrophysics Data System (ADS)

    Bayanna, A. Raja; Louis, Rohan Eugene; Kumar, Brajesh; Mathew, Shibu K.; Venkatakrishnan, P.

    2007-09-01

    A Multi-Application Solar Telescope (MAST) is proposed to be installed at the Udaipur Solar Observatory (USO) in India to monitor the Sun in optical and near infra-red wavelengths. The median value of the Fried's parameter at this site is 4 cm. USO is in the process of building an Adaptive optics (AO) system in order to have diffraction limited performance of the MAST under this moderate seeing condition. AO helps in achieving high-resolution imaging by compensating the atmospheric turbulence in real-time. We have performed simulations to evaluate the performance of AO for various seeing conditions. It was concluded that with the present availability of AO system components, a 55 cm aperture telescope would yield optimum performance with AO, in combination with post-processing techniques like speckle imaging and phase diversity. At present, we are developing a proto-type AO system at USO to demonstrate its performance with a 15 cm Coudé refracting telescope as a preparation for the main AO system to be deployed on the MAST. The prototype AO system is being realized in two phases. In the first phase, we have developed an image stabilization system to compensate the global tilt of the wave-front. The second phase consists of sensing and correcting the local tilts of the wave-front by integrating a micro-machined membrane deformable mirror with the image stabilization system and is currently in progress. Here, we present the details of our proto-type AO system. We also present preliminary results obtained from simulations using Phase Diversity as a post processing technique.

  7. The standardization of super resolution optical microscopic images based on DICOM

    NASA Astrophysics Data System (ADS)

    Xia, Wei; Gao, Xin

    2015-03-01

    Super resolution optical microscopy allows the capture of images with a higher resolution than the diffraction limit. However, due to the lack of a standard format, the processing, visualization, transfer, and exchange of Super Resolution Optical Microscope (SROM) images are inconvenient. In this work, we present an approach to standardize the SROM images based on the Digital Imaging and Communication in Medicine (DICOM) standard. The SROM images and associated information are encapsulated and converted to DICOM images based on the Visible Light Microscopic Image Information Object Definition of DICOM. The new generated SROM images in DICOM format can be displayed, processed, transferred, and exchanged by using most medical image processing tools.

  8. A Conflict Resolution Model

    ERIC Educational Resources Information Center

    Davidson, John; Wood, Christine

    2004-01-01

    The Conflict Resolution Model was formulated by a group of Australian psychologists who set about integrating the literature on achieving mutually beneficial outcomes in a conflict situation in order to create a best-practice prescriptive process for conflict resolution. A number of experimental studies conducted at the University of Tasmania with…

  9. High-resolution full-field optical coherence tomography using high dynamic range image processing

    NASA Astrophysics Data System (ADS)

    Leong-Hoï, A.; Claveau, R.; Montgomery, P. C.; Serio, B.; Uhring, W.; Anstotz, F.; Flury, M.

    2016-04-01

    Full-field optical coherence tomography (FF-OCT) based on white-light interference microscopy, is an emerging noninvasive imaging technique for characterizing biological tissue or optical scattering media with micrometer resolution. Tomographic images can be obtained by analyzing a sequence of interferograms acquired with a camera. This is achieved by scanning an interferometric microscope objectives along the optical axis and performing appropriate signal processing for fringe envelope extraction, leading to three-dimensional imaging over depth. However, noise contained in the images can hide some important details or induce errors in the size of these details. To firstly reduce temporal and spatial noise from the camera, it is possible to apply basic image post processing methods such as image averaging, dark frame subtraction or flat field division. It has been demonstrate that this can improve the quality of microscopy images by enhancing the signal to noise ratio. In addition, the dynamic range of images can be enhanced to improve the contrast by combining images acquired with different exposure times or light intensity. This can be made possible by applying a hybrid high dynamic range (HDR) technique, which is proposed in this paper. High resolution tomographic analysis is thus performed using a combination of the above-mentioned image processing techniques. As a result, the lateral resolution of the system can be improved so as to approach the diffraction limit of the microscope as well as to increase the power of detection, thus enabling new sub-diffraction sized structures contained in a transparent layer, initially hidden by the noise, to be detected.

  10. High resolution projection micro stereolithography system and method

    SciTech Connect

    Spadaccini, Christopher M.; Farquar, George; Weisgraber, Todd; Gemberling, Steven; Fang, Nicholas; Xu, Jun; Alonso, Matthew; Lee, Howon

    2016-11-15

    A high-resolution P.mu.SL system and method incorporating one or more of the following features with a standard P.mu.SL system using a SLM projected digital image to form components in a stereolithographic bath: a far-field superlens for producing sub-diffraction-limited features, multiple spatial light modulators (SLM) to generate spatially-controlled three-dimensional interference holograms with nanoscale features, and the integration of microfluidic components into the resin bath of a P.mu.SL system to fabricate microstructures of different materials.

  11. High resolution imaging at Palomar

    NASA Technical Reports Server (NTRS)

    Kulkarni, Shrinivas R.

    1992-01-01

    For the last two years we have embarked on a program of understanding the ultimate limits of ground-based optical imaging. We have designed and fabricated a camera specifically for high resolution imaging. This camera has now been pressed into service at the prime focus of the Hale 5 m telescope. We have concentrated on two techniques: the Non-Redundant Masking (NRM) and Weigelt's Fully Filled Aperture (FFA) method. The former is the optical analog of radio interferometry and the latter is a higher order extension of the Labeyrie autocorrelation method. As in radio Very Long Baseline Interferometry (VLBI), both these techniques essentially measure the closure phase and, hence, true image construction is possible. We have successfully imaged binary stars and asteroids with angular resolution approaching the diffraction limit of the telescope and image quality approaching that of a typical radio VLBI map. In addition, we have carried out analytical and simulation studies to determine the ultimate limits of ground-based optical imaging, the limits of space-based interferometric imaging, and investigated the details of imaging tradeoffs of beam combination in optical interferometers.

  12. Nonlinear spectroscopy in the near-field: time resolved spectroscopy and subwavelength resolution non-invasive imaging

    NASA Astrophysics Data System (ADS)

    Namboodiri, Mahesh; Khan, Tahirzeb; Karki, Khadga; Kazemi, Mehdi Mohammad; Bom, Sidhant; Flachenecker, Günter; Namboodiri, Vinu; Materny, Arnulf

    2014-04-01

    The combination of near-field microscopy along with nonlinear optical spectroscopic techniques is presented here. The scanning near-field imaging technique can be integrated with nonlinear spectroscopic techniques to improve spatial and axial resolution of the images. Additionally, ultrafast dynamics can be probed down to nano-scale dimension. The review shows some examples for this combination, which resulted in an exciton map and vibrational contrast images with sub-wavelength resolution. Results of two-color femtosecond time-resolved pump-probe experiments using scanning near-field optical microscopy (SNOM) on thin films of the organic semiconductor 3,4,9,10 Perylenetetracarboxylic dianhydride (PTCDA) are presented. While nonlinear Raman techniques have been used to obtain highly resolved images in combination with near-field microscopy, the use of femtosecond laser pulses in electronic resonance still constitutes a big challenge. Here, we present our first results on coherent anti-Stokes Raman scattering (fs-CARS) with femtosecond laser pulses detected in the near-field using SNOM. We demonstrate that highly spatially resolved images can be obtained from poly(3-hexylthiophene) (P3HT) nano-structures where the fs-CARS process was in resonance with the P3HT absorption and with characteristic P3HT vibrational modes without destruction of the samples. Sub-diffraction limited lateral resolution is achieved. Especially the height resolution clearly surpasses that obtained with standard microCARS. These results will be the basis for future investigations of mode-selective dynamics in the near-field.

  13. USGS aerial resolution targets.

    USGS Publications Warehouse

    Salamonowicz, P.H.

    1982-01-01

    It is necessary to measure the achievable resolution of any airborne sensor that is to be used for metric purposes. Laboratory calibration facilities may be inadequate or inappropriate for determining the resolution of non-photographic sensors such as optical-mechanical scanners, television imaging tubes, and linear arrays. However, large target arrays imaged in the field can be used in testing such systems. The USGS has constructed an array of resolution targets in order to permit field testing of a variety of airborne sensing systems. The target array permits any interested organization with an airborne sensing system to accurately determine the operational resolution of its system. -from Author

  14. Shedding new light on viruses: super-resolution microscopy for studying human immunodeficiency virus.

    PubMed

    Müller, Barbara; Heilemann, Mike

    2013-10-01

    For more than 70 years electron microscopy (EM) techniques have played an important role in investigating structures of enveloped viruses. By contrast, use of fluorescence microscopy (FM) methods for this purpose was limited by the fact that the size of virus particles is generally around or below the diffraction limit of light microscopy. Various super-resolution (SR) fluorescence imaging techniques developed over the past two decades bypass the diffraction limit of light microscopy, allowing visualization of subviral details and bridging the gap between conventional FM and EM methods. We summarize here findings on human immunodeficiency virus (HIV-1) obtained using SR-FM techniques. Although the number of published studies is currently limited and some of the pioneering analyses also covered methodological or descriptive aspects, recent publications clearly indicate the potential to approach open questions in HIV-1 replication from a new angle.

  15. Every Good Virtue You Ever Wanted in a Q-switched Solid-state Laser and More: Monolithic, Diode-pumped, Self-q-switched, Highly Reproducible, Diffraction-limited Nd:yag Laser

    NASA Technical Reports Server (NTRS)

    Chen, Y. C.; Lee, K. K.

    1993-01-01

    The applications of Q-switched lasers are well known, for example, laser radar, laser remote sensing, satellite orbit determination, Moon orbit and 'moon quake' determination, satellite laser communication, and many nonlinear optics applications. Most of the applications require additional properties of the Q-switched lasers, such as single-axial and/or single-transverse mode, high repetition rate, stable pulse shape and pulse width, or ultra compact and rugged oscillators. Furthermore, space based and airborne lasers for lidar and laser communication applications require efficient, compact, lightweight, long-lived, and stable-pulsed laser sources. Diode-pumped solid-state lasers (DPSSL) have recently shown the potential for satisfying all of these requirements. We will report on the operating characteristics of a diode-pumped, monolithic, self-Q-switched Cr,Nd:YAG laser where the chromium ions act as a saturable absorber for the laser emission at 1064 nm. The pulse duration is 3.5 ns and the output is highly polarized with an extinction ratio of 700:1. It is further shown that the output is single-longitudinal-mode with transform-limited spectral line width without pulse-to-pulse mode competition. Consequently, the pulse-to-pulse intensity fluctuation is less than the instrument resolution of 0.25 percent. This self-stabilization mechanism is because the lasing mode bleaches the distributed absorber and establishes a gain-loss grating similar to that used in the distributed feedback semiconductor lasers. A repetition rate above 5 KHz has also been demonstrated. For higher power, this laser can be used for injection seeding an amplifier (or amplifier chain) or injection locking of a power oscillator pumped by diode lasers. We will discuss some research directions on the master oscillator for higher output energy per pulse as well as how to scale the output power of the diode-pumped amplifier(s) to multi-kilowatt average power.

  16. Focal plane actuation by hexapod for the development of a high-resolution suborbital telescope

    NASA Astrophysics Data System (ADS)

    Miller, Alexander D.; Scowen, Paul A.; Veach, Todd J.

    2016-07-01

    We present a prototype hexapod image stabilization system as the key instrument for a proposed suborbital balloon mission. The unique design thermally isolates an off-the-shelf non-cryogenic hexapod from a liquid nitrogen cooled focal plane, enabling its use in a cryogenic environment. Balloon gondolas currently achieve 1-2 arcsecond pointing error, but cannot correct for unavoidable jitter movements ( 20 micron amplitude at 20 Hz at the worst) caused by wind rushing over balloon surfaces, thermal variations, and vibrations from cryocoolers, and reaction wheels. The jitter causes image blur during exposures and limits the resolution of the system. Removal of this final jitter term decreases pointing error by an order of magnitude and allows for true diffraction-limited observation. Tip-tilt pointing systems have been used for these purposes in the past, but require additional optics and introduce multiple reflections. The hexapod system, rather, is compact and can be plugged into the focal point of nearly any configuration. For a 0.8m telescope the improvement in resolution by this system would provide 0.1" angular resolution at 300nm, which is comparable to Hubble for a fraction of the cost. On an actual balloon, the hexapod system would actuate the focal plane to counteract the jitter using position information supplied by guidestar cameras. However, in the lab, we instead simulate guide camera tracking, using a 1024 × 1024 e2v science-grade CCD to take long exposures of a target attached to an XY stage driven with the balloon jitter signal recorded during the STO mission. Further confirmation of the positional accuracy and agility of the hexapod is achieved using a laser and fast-sampling position-sensitive diode. High-resolution time domain multispectral imaging of the gas giants, especially in the UV range, is of particular interest to the planetary community, and a suborbital telescope with the hexapod stabilization in place would provide a wealth of new

  17. High Resolution X-ray Imaging

    NASA Technical Reports Server (NTRS)

    Cash, Webster

    2002-01-01

    NAG5-5020 covered a period of 7.5 years during which a great deal of progress was made in x-ray optical techniques under this grant. We survived peer review numerous times during the effort to keep the grant going. In 1994, when the grant started we were actively pursuing the application of spherical mirrors to improving x-ray telescopes. We had found that x-ray detectors were becoming rapidly more sophisticated and affordable, but that x-ray telescopes were only being improved through the intense application of money within the AXAF program. Clearly new techniques for the future were needed. We were successful in developing and testing at the HELSTF facility in New Mexico a four reflection coma-corrected telescope made from spheres. We were able to demonstrate 0.3 arcsecond resolution, almost to the diffraction limit of the system. The community as a whole was, at that time, not particularly interested in looking past AXAF (Chandra) and the effort needed to evolve. Since we had reached the diffraction limit using non-Wolter optics we then decided to see if we could build an x-ray interferometer in the laboratory. In the lab the potential for improved resolution was substantial. If synthetic aperture telescopes could be built in space, then orders of magnitude improvement would become feasible. In 1998 NASA, under the direction of Dr. Nick White of Goddard, started a study to assess the potential and feasibility of x-ray interferometry in space. My work became of central interest to the committee because it indicated that such was possible. In early 1999 we had the breakthrough that allowed us build a practical interferometer. By using flats and hooking up with the Marshall Space Flight Center facilities we were able to demonstrate fringes at 1.25keV on a one millimeter baseline. This actual laboratory demonstration provided the solid proof of concept that NASA needed.

  18. High Spatial Resolution Spectroscopy of Semiconductor Nanostructures

    NASA Astrophysics Data System (ADS)

    Harris, Timothy D.; Gershoni, David; Pfeiffer, Loren N.

    1996-03-01

    Several recent reports employing high spatial resolution have revealed the dominance of exciton localization in the low temperature luminescence of semiconductor quantum structures.^[1-3] Understanding this localization is of critical importance for the reliable studies of low dimensional structures such as quantum wells, quantum wires and quantum dots. We report on low temperature and high spatial resolution photoluminescence and photoluminescence excitation studies of cleaved edge overgrown (CEO) single quantum wires. These samples permit the direct and unambiguous comparison between the optical properties of a (100) oriented quantum well, a (110) oriented quantum well, and the quantum wire which is formed at their intersection. Using low temperature near field optical spectroscopy, and a novel diffraction limited far field apparatus, we determine the carrier diffusion length dependence on pump wavelength and sample temperature in both the 2d systems and the genuinely 1D wire system. We also measure the absorption strength of the 1D system and find it to be a factor of 3 stronger than the absorption of the associated 2D systems.^[2] Using low temperature near field optical spectroscopy, and a novel diffraction limited far field apparatus, we also determine the carrier diffusion length dependence on pump wavelength and sample temperature. ^[1] H. F. Hess, E. Betzig, T. D. Harris, L. N. Pfeiffer, and K. W. West, Science 264, 1740 (1994). ^[2] T. D. Harris, D. Gershoni, R. D. Grober, L. Pfeiffer, K. West, and N. Chand, Appl. Phys. Lett, in press (1996) ^[3] D. Gammon, E. S. Snow, and D. S. Katzer, Appl. Phys. Lett. 67, 2391 (1995)

  19. High-resolution imaging of the Pluto-Charon system with the Faint Object Camera of the Hubble Space Telescope

    NASA Technical Reports Server (NTRS)

    Albrecht, R.; Barbieri, C.; Adorf, H.-M.; Corrain, G.; Gemmo, A.; Greenfield, P.; Hainaut, O.; Hook, R. N.; Tholen, D. J.; Blades, J. C.

    1994-01-01

    Images of the Pluto-Charon system were obtained with the Faint Object Camera (FOC) of the Hubble Space Telescope (HST) after the refurbishment of the telescope. The images are of superb quality, allowing the determination of radii, fluxes, and albedos. Attempts were made to improve the resolution of the already diffraction limited images by image restoration. These yielded indications of surface albedo distributions qualitatively consistent with models derived from observations of Pluto-Charon mutual eclipses.

  20. Improved Spatial Resolution for Reflection Mode Infrared Microscopy

    SciTech Connect

    Bechtel, Hans A.; Martin, Michael C.; May, T.E.; Lerch, Philippe

    2009-10-09

    Standard commercial infrared microscopes operating in reflection mode use a mirror to direct the reflected light from the sample to the detector. This mirror blocks about half of the incident light, however, and thus degrades the spatial resolution by reducing the umerical aperture of the objective. Here, we replace the mirror with a 50% beamsplitter to allow full illumination of the objective and retain a way to direct the reflected light to the detector. The improved spatial resolution is demonstrated using two different microscopes apable of diffraction-limited resolution: the first microscope is coupled to a synchrotron source and utilizes a single point detector, whereas the second microscope has a standard blackbody source and uses a focal planetarray (FPA) detector.

  1. Efficient phase contrast imaging in STEM using a pixelated detector. Part 1: Experimental demonstration at atomic resolution

    DOE PAGES

    Pennycook, Timothy J.; Lupini, Andrew R.; Yang, Hao; ...

    2014-10-15

    In this paper, we demonstrate a method to achieve high efficiency phase contrast imaging in aberration corrected scanning transmission electron microscopy (STEM) with a pixelated detector. The pixelated detector is used to record the Ronchigram as a function of probe position which is then analyzed with ptychography. Ptychography has previously been used to provide super-resolution beyond the diffraction limit of the optics, alongside numerically correcting for spherical aberration. Here we rely on a hardware aberration corrector to eliminate aberrations, but use the pixelated detector data set to utilize the largest possible volume of Fourier space to create high efficiency phasemore » contrast images. The use of ptychography to diagnose the effects of chromatic aberration is also demonstrated. In conclusion, the four dimensional dataset is used to compare different bright field detector configurations from the same scan for a sample of bilayer graphene. Our method of high efficiency ptychography produces the clearest images, while annular bright field produces almost no contrast for an in-focus aberration-corrected probe.« less

  2. Efficient phase contrast imaging in STEM using a pixelated detector. Part 1: Experimental demonstration at atomic resolution

    SciTech Connect

    Pennycook, Timothy J.; Lupini, Andrew R.; Yang, Hao; Murfitt, Matthew F.; Jones, Lewys; Nellist, Peter D.

    2014-10-15

    In this paper, we demonstrate a method to achieve high efficiency phase contrast imaging in aberration corrected scanning transmission electron microscopy (STEM) with a pixelated detector. The pixelated detector is used to record the Ronchigram as a function of probe position which is then analyzed with ptychography. Ptychography has previously been used to provide super-resolution beyond the diffraction limit of the optics, alongside numerically correcting for spherical aberration. Here we rely on a hardware aberration corrector to eliminate aberrations, but use the pixelated detector data set to utilize the largest possible volume of Fourier space to create high efficiency phase contrast images. The use of ptychography to diagnose the effects of chromatic aberration is also demonstrated. In conclusion, the four dimensional dataset is used to compare different bright field detector configurations from the same scan for a sample of bilayer graphene. Our method of high efficiency ptychography produces the clearest images, while annular bright field produces almost no contrast for an in-focus aberration-corrected probe.

  3. High-density channel model and detection method for signal readout from super-resolution near-field structure discs

    NASA Astrophysics Data System (ADS)

    Hosogai, Shota; Ansai, Tsutomu; Yoshinari, Takehisa; Tanabe, Takaya

    2016-09-01

    Although a readout method using the super-resolution near-field structure (super-RENS) effect can overcome diffraction limits, readout characteristics for greatly surpassed high-density conditions do not become clear, because a high-density channel function having a differential response property is superimposed on a normal readout function. We propose a high-density channel model to indicate the properties of the super-RENS effect directly. This model can be expressed as a differential response function using the finite impulse response (FIR) filter model. It expresses the super-RENS readout process, which is divided on the basis of recording densities such as high and normal Blu-ray Disc™ densities. We estimated the properties of super-RENS readout signals by comparison between theoretical expressions and experiments. Results show that good signal quality require readout signals having sharp peaks and smaller offsets. We also evaluated the channel model by adding an adaptive FIR filter and a Viterbi decoder by simulations. Results show that the super-RENS disc can achieve a fourfold higher recording density if the signal-to-noise ratio (S/N) is improved to 6 dB in the case of partial response (PR) (1 + D + D 2).

  4. Controllable fabrication of super-resolution nanocrater arrays by laser direct writing.

    PubMed

    Wang, Yongsheng; Guo, Chuanfei; Cao, Sihai; Miao, Junjie; Ren, Tianling; Liu, Qian

    2010-11-01

    A super-resolution fabrication technique for highly-ordered nanocrater arrays is reported based on laser direct writing method. Nanocraters with diameters up to 40 nm, which is much smaller than the diffraction limit of laser system, are fabricated on titanium films using a 532 nm laser. The diameters of the craters are tunable from 350 nm to 40 nm, with a rigorous linear relationship versus the writing laser powers and an approximate linear relationship versus pulse widths. Laser ablation and oxidation are involved in formation mechanism and Gaussian distribution of laser energy density is proposed to play a key role of super-resolution structures.

  5. Super-resolution optical microscopy based on scannable cantilever-combined microsphere.

    PubMed

    Wang, Shuying; Zhang, Dongxian; Zhang, Haijun; Han, Xu; Xu, Rui

    2015-12-01

    We report an ingenious method of super-resolution optical microscopy utilizing scannable cantilever-combined microsphere. By scanning the microsphere over the sample surface in a cantilever-combined microsphere-sample contact state, super-resolution images can be acquired at arbitrary sample regions through near-field information collection by the microsphere. In addition, such a state can effectively reduce the possibility of breaking the cantilever and damaging the microsphere or sample surface. This work has developed a new method and technique of sub-diffraction-limit optical microscopy, and can be practically applied in various fields of micro/nanoscopy.

  6. Nanometric resolution using far-field optical tomographic microscopy in the multiple scattering regime

    SciTech Connect

    Girard, Jules; Maire, Guillaume; Giovannini, Hugues; Belkebir, Kamal; Chaumet, Patrick C.; Sentenac, Anne; Talneau, Anne

    2010-12-15

    The resolution of optical far-field microscopes is classically diffraction-limited to half the illumination wavelength. We show experimentally that this fundamental limit does not apply in the multiple scattering regime. We used tomographic diffractive microscopy at 633 nm to image two pairs of closely spaced rods (with a width and interdistance of 50 nm) of widely different diffractive properties. Using an inversion algorithm accounting for multiple scattering, only the pair of highly diffracting rods could be clearly visualized with a resolution similar to that of an atomic force microscope.

  7. High resolution studies of complex solar active regions

    NASA Astrophysics Data System (ADS)

    Deng, Na

    Flares and Coronal Mass Ejections (CMEs) are energetic events, which can even impact the near-Earth environment and are the principal source of space weather. Most of them originate in solar active regions. The most violent events are produced in sunspots with a complex magnetic field topology. Studying their morphology and dynamics is helpful in understanding the energy accumulation and release mechanisms for flares and CMEs, which are intriguing problems in solar physics. The study of complex active regions is based on high-resolution observations from space missions and new instruments at the Big Bear Solar Observatory (BBSO). Adaptive optics (AO) in combination with image restoration techniques (speckle masking imaging) can achieve improved image quality and a spatial resolution (about 100 km on the solar surface) close to the diffraction limit of BBSO's 65 cm vacuum telescope. Dopplergrams obtained with a two-dimensional imaging spectrometer combined with horizontal flow maps derived with Local Correlation Tracking (LCT) provide precise measurements of the three-dimensional velocity field in sunspots. Magnetic field measurements from ground- and space-based instruments complement these data. At the outset of this study, the evolution and morphology of a typical round sunspot are described in some detail. The sunspot was followed from disk center to the limb, thus providing some insight into the geometry of the magnetic flux system. Having established a benchmark for a stable sunspot, the attention is turned to changes of the sunspot structure associated with flares and CMEs. Rapid penumbral decay and the strengthening of sunspot umbrae are manifestations of photospheric magnetic field changes after a flare. These sudden intensity changes are interpreted as a result of magnetic reconnection during the flare, which causes the magnetic field lines to be turned from more inclined to more vertical. Strong photospheric shear flows along the flaring magnetic

  8. Novel fluidic packaging of gimbal-less MEMS mirrors for increased optical resolution and overall performance

    NASA Astrophysics Data System (ADS)

    Milanovic, Veljko; Kasturi, Abhishek; Yang, James

    2016-05-01

    Gimbal-less two-axis quasistatic MEMS mirrors have the ability to reflect optical beams to arbitrary positions and with arbitrary velocity. This technology has become established in many applications including laser based tracking, 3D scanning, biomedical imaging, free-space communication, and LiDAR. However, for certain defense applications, the total angle × diameter product, or the mirror's effective achievable resolution (θ*D product), has not been large enough to address requirements for agile steering in large fields of regard and with a low diffraction-limited beam divergence. Two key limitations have been the relatively low forces available in electrostatic combdrive actuators and the susceptibility of large-diameter MEMS mirrors to shock and vibrations. In this work, we demonstrate that these same MEMS mirrors can have dramatically increased performance when fully immersed and packaged in dielectric liquids with highly favorable torque-increasing, damping-increasing, and optical gain-increasing properties. The rotating electrostatic combdrive has its torque multiplied by liquid's relative permittivity of ~2.5. Furthermore, by selecting the appropriate fluid viscosity, quality factor of the device is reduced and structural damping is tuned to near critical damping. Finally, the increased scan angle due to the ~1.5-1.7 index of refraction of the fluid is an additional benefit. These numerous benefits of the fluidic packaging enabled us to double and in some cases triple the previously achieved θ*D product of two-axis quasistatic MEMS mirrors while still maintaining speeds applicable for above mentioned applications. One of the most exciting benefits of the packaging methodologies is that the damping dramatically increases shock and vibration tolerance, which will be tested next.

  9. Diffraction-Limited Imaging of Space Objects III.

    DTIC Science & Technology

    1986-10-01

    number of disciplines, including astronomy, x - ray crystallography, electron microscopy and wavefront sensing, one encounters the phase -retrieval problem...object [25]: one can only narrow down the possibilities. (iii) Difference Fourier Synthesis Some of the phase retrieval methods from x - ray ...to complex-valued objects. INTRODUCTION Several phase -retrieval algorithms have been demonstrat- In a number of disciplines, including astronomy, x - ray

  10. Imaging atoms from resonance fluorescence spectrum beyond the diffraction limit

    NASA Astrophysics Data System (ADS)

    Liao, Zeyang; Al-Amri, Mohammad; Zubairy, M. Suhail

    2014-03-01

    We calculate the resonance fluorescence spectrum of a linear chain of two-level atoms driven by a gradient coherent laser field. The result shows that we can determine the positions of atoms from the spectrum even when the atoms locate within subwavelength range and the dipole-dipole interaction is significant. This far-field resonance fluorescence localization microscopy method does not require point-by-point scanning and it may be more time-efficient. We also give a possible scheme to extract the position information in an extended region without requiring more peak power of laser. We also briefly discuss how to do a 2D imaging based on our scheme. This work is supported by grants from the King Abdulaziz City for Science and Technology (KACST) and the Qatar National Research Fund (QNRF) under the NPRP project.

  11. Diffraction Limited Performance of Infra Red Fresnel Lenses.

    DTIC Science & Technology

    The effect of diffraction on Fresnel lens performance has been calculated. It is shown that the bandwidth of a coherent lens is very narrow. For low...monochromatic (laser) applications, on the other hand, the Fresnel lens may offer significant cost advantages over the conventional alternatives.

  12. The diffraction limit of an optical spectrum analyzer

    NASA Astrophysics Data System (ADS)

    Kolobrodov, V. G.; Tymchik, G. S.; Kolobrodov, M. S.

    2015-11-01

    This article examines a systematic error that occurs in optical spectrum analyzers and is caused by Fresnel approximation. The aim of the article is to determine acceptable errors of spatial frequency measurement in signal spectrum. The systematic error of spatial frequency measurement has been investigated on the basis of a physical and mathematical model of a coherent spectrum analyzer. It occurs as a result of the transition from light propagation in free space to Fresnel diffraction. Equations used to calculate absolute and relative measurement errors depending on a diffraction angle have been obtained. It allows us to determine the limits of the spectral range according to the given relative error of the spatial frequency measurement.

  13. Initiator Diffraction Limits for Pulse Detonation Engine Operation

    DTIC Science & Technology

    2002-12-01

    12 Figure 9. Location of 3.39 µm He-Ne Laser Transmission...transmission measurements were made at two locations with a 3.39 µm He-Ne laser [Figure 9] and an infrared diode to determine the proper timing of the...the main combustor [Figure 10]. Figure 9. Location of 3.39 µm He-Ne Laser Transmission Figure 10. Transducer Locations 13 Various other

  14. Seeing the forest tree by tree: super-resolution light microscopy meets the neurosciences.

    PubMed

    Maglione, Marta; Sigrist, Stephan J

    2013-07-01

    Light microscopy can be applied in vivo and can sample large tissue volumes, features crucial for the study of single neurons and neural circuits. However, light microscopy per se is diffraction-limited in resolution, and the substructure of core signaling compartments of neuronal circuits--axons, presynaptic active zones, postsynaptic densities and dendritic spines-can be only insufficiently characterized by standard light microscopy. Recently, several forms of super-resolution light microscopy breaking the diffraction-imposed resolution limit have started to allow highly resolved, dynamic imaging in the cell-biologically highly relevant 10-100 nanometer range ('mesoscale'). New, sometimes surprising answers concerning how protein mobility and protein architectures shape neuronal communication have already emerged. Here we start by briefly introducing super-resolution microscopy techniques, before we describe their use in the analysis of neuronal compartments. We conclude with long-term prospects for super-resolution light microscopy in the molecular and cellular neurosciences.

  15. Optical far-field super-resolution microscopy using nitrogen vacancy center ensemble in bulk diamond

    NASA Astrophysics Data System (ADS)

    Li, Shen; Chen, Xiang-dong; Zhao, Bo-Wen; Dong, Yang; Zou, Chong-Wen; Guo, Guang-Can; Sun, Fang-Wen

    2016-09-01

    We demonstrate optical far-field super-resolution microscopy using an array of nitrogen vacancy centers in bulk diamond as near-field optical probes. The local optical field, which transmits through the nanostructures on the diamond surface, is measured by detecting the charge state conversion of the nitrogen vacancy center. Locating the nitrogen vacancy center with a spatial resolution of 6.1 nm is realized with charge state depletion nanoscopy. The nanostructures on the surface of a diamond are then imaged with a resolution below the optical diffraction limit. The results offer an approach to build a general-purpose optical super-resolution microscopy technique and a convenient platform for high spatial resolution quantum sensing with nitrogen vacancy centers.

  16. Maskless plasmonic lithography at 22 nm resolution.

    PubMed

    Pan, Liang; Park, Yongshik; Xiong, Yi; Ulin-Avila, Erick; Wang, Yuan; Zeng, Li; Xiong, Shaomin; Rho, Junsuk; Sun, Cheng; Bogy, David B; Zhang, Xiang

    2011-01-01

    Optical imaging and photolithography promise broad applications in nano-electronics, metrologies, and single-molecule biology. Light diffraction however sets a fundamental limit on optical resolution, and it poses a critical challenge to the down-scaling of nano-scale manufacturing. Surface plasmons have been used to circumvent the diffraction limit as they have shorter wavelengths. However, this approach has a trade-off between resolution and energy efficiency that arises from the substantial momentum mismatch. Here we report a novel multi-stage scheme that is capable of efficiently compressing the optical energy at deep sub-wavelength scales through the progressive coupling of propagating surface plasmons (PSPs) and localized surface plasmons (LSPs). Combining this with airbearing surface technology, we demonstrate a plasmonic lithography with 22 nm half-pitch resolution at scanning speeds up to 10 m/s. This low-cost scheme has the potential of higher throughput than current photolithography, and it opens a new approach towards the next generation semiconductor manufacturing.

  17. Next generation high resolution adaptive optics fundus imager

    NASA Astrophysics Data System (ADS)

    Fournier, P.; Erry, G. R. G.; Otten, L. J.; Larichev, A.; Irochnikov, N.

    2005-12-01

    The spatial resolution of retinal images is limited by the presence of static and time-varying aberrations present within the eye. An updated High Resolution Adaptive Optics Fundus Imager (HRAOFI) has been built based on the development from the first prototype unit. This entirely new unit was designed and fabricated to increase opto-mechanical integration and ease-of-use through a new user interface. Improved camera systems for the Shack-Hartmann sensor and for the scene image were implemented to enhance the image quality and the frequency of the Adaptive Optics (AO) control loop. An optimized illumination system that uses specific wavelength bands was applied to increase the specificity of the images. Sample images of clinical trials of retinas, taken with and without the system, are shown. Data on the performance of this system will be presented, demonstrating the ability to calculate near diffraction-limited images.

  18. High resolution drift chambers

    SciTech Connect

    Va'vra, J.

    1985-07-01

    High precision drift chambers capable of achieving less than or equal to 50 ..mu..m resolutions are discussed. In particular, we compare so called cool and hot gases, various charge collection geometries, several timing techniques and we also discuss some systematic problems. We also present what we would consider an ''ultimate'' design of the vertex chamber. 50 refs., 36 figs., 6 tabs.

  19. Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging.

    PubMed

    Kisley, Lydia; Brunetti, Rachel; Tauzin, Lawrence J; Shuang, Bo; Yi, Xiyu; Kirkeminde, Alec W; Higgins, Daniel A; Weiss, Shimon; Landes, Christy F

    2015-09-22

    Porous materials such as cellular cytosol, hydrogels, and block copolymers have nanoscale features that determine macroscale properties. Characterizing the structure of nanopores is difficult with current techniques due to imaging, sample preparation, and computational challenges. We produce a super-resolution optical image that simultaneously characterizes the nanometer dimensions of and diffusion dynamics within porous structures by correlating stochastic fluctuations from diffusing fluorescent probes in the pores of the sample, dubbed here as "fluorescence correlation spectroscopy super-resolution optical fluctuation imaging" or "fcsSOFI". Simulations demonstrate that structural features and diffusion properties can be accurately obtained at sub-diffraction-limited resolution. We apply our technique to image agarose hydrogels and aqueous lyotropic liquid crystal gels. The heterogeneous pore resolution is improved by up to a factor of 2, and diffusion coefficients are accurately obtained through our method compared to diffraction-limited fluorescence imaging and single-particle tracking. Moreover, fcsSOFI allows for rapid and high-throughput characterization of porous materials. fcsSOFI could be applied to soft porous environments such hydrogels, polymers, and membranes in addition to hard materials such as zeolites and mesoporous silica.

  20. Non-contact distance measurement and profilometry using thermal near-field radiation towards a high resolution inspection and metrology solution

    NASA Astrophysics Data System (ADS)

    Bijster, Roy; Sadeghian, Hamed; van Keulen, Fred

    2016-03-01

    Optical near-field technologies such as solid immersion lenses and hyperlenses are candidate solutions for high resolution and high throughput wafer inspection and metrology for the next technology nodes. Besides sub-diffraction limited optical performance, these concepts share the necessity of extreme proximity to the sample at distances that are measured in tens of nanometers. For the instrument this poses two major challenges: 1) how to measure the distance to the sample? and 2) how to position accurately and at high speed? For the first challenge near-field thermal radiation is proposed as a mechanism for an integrated distance sensor (patent pending). This sensor is realized by making a sensitive calorimeter (accuracy of 2:31nW root sum squared). When used for distance measurement an equivalent uncertainty of 1nm can be achieved for distances smaller than 100 nm. By scanning the distance sensor over the sample, thermal profilometry is realized, which can be used to inspect surfaces in a non-intrusive and non-contact way. This reduces wear of the probe and minimizes the likelihood of damaging the sample.

  1. High-Resolution and Lightweight X-ray Optics for the X-Ray Surveyor

    NASA Astrophysics Data System (ADS)

    Zhang, William

    Envisioned in "Enduring Quest, Daring Visions" and under study by NASA as a potential major mission for the 2020s, the X-ray Surveyor mission will likely impose three requirements on its optics: (1) high angular resolution: 0.5 PSF, (2) large effective area: e10,000 cm2 or more, and (3) affordable production cost: $500M. We propose a technology that can meet these requirements by 2020. It will help the X-ray Surveyor secure the endorsement of the coming decadal survey and enable its implementation following WFIRST. The technology comprises four elements: (1) fabrication of lightweight single crystal silicon mirrors, (2) coating these mirrors with iridium to maximize effective area without figure degradation, (3) alignment and bonding of these mirrors to form meta-shells that will be integrated to make a mirror assembly, and (4) systems engineering to ensure that the mirror assembly meet all science performance and spaceflight environmental requirements. This approach grows out of our existing approach based on glass slumping. Using glass slumping technology, we have been able to routinely build and test mirror modules of 10half-power diameter (HPD). While comparable in HPD to XMM-Newtons electroformed nickel mirrors, these mirror modules are 10 times lighter. Likewise, while comparable in weight to Suzakus epoxy-replicated aluminum foil mirrors, these modules have 10 times better HPD. These modules represent the current state of the art of lightweight X-ray optics. Although both successful and mature, the glass slumping technology has reached its limit and cannot achieve sub-arc second HPD. Therefore, we are pursuing the new approach based on polishing single crystal silicon. The new approach will enable the building and testing of mirror modules, called meta-shells, capable of 3HPD by 2018 and 1HPD by 2020, and has the potential to reach diffraction limits ( 0.1) in the 2020s.

  2. Graded Achievement, Tested Achievement, and Validity

    ERIC Educational Resources Information Center

    Brookhart, Susan M.

    2015-01-01

    Twenty-eight studies of grades, over a century, were reviewed using the argument-based approach to validity suggested by Kane as a theoretical framework. The review draws conclusions about the meaning of graded achievement, its relation to tested achievement, and changes in the construct of graded achievement over time. "Graded…

  3. Measuring charge carrier mobility in photovoltaic devices with micron-scale resolution

    SciTech Connect

    Ashraf, A.; Dissanayake, D. M. N. M.; Eisaman, M. D.

    2015-03-16

    We present a charge-extraction technique, micron-scale charge extraction by linearly increasing voltage, which enables simultaneous spatially resolved measurements of charge carrier mobility and photocurrent in thin-film photovoltaic devices with micron-scale resolution. An intensity-modulated laser with beam diameter near the optical diffraction limit is scanned over the device, while a linear voltage ramp in reverse bias is applied at each position of illumination. We calculate the majority carrier mobility, photocurrent, and number of photogenerated charge carriers from the resulting current transient. We demonstrate this technique on an organic photovoltaic device, but it is applicable to a wide range of photovoltaic materials.

  4. High resolution 3D imaging of living cells with sub-optical wavelength phonons

    NASA Astrophysics Data System (ADS)

    Pérez-Cota, Fernando; Smith, Richard J.; Moradi, Emilia; Marques, Leonel; Webb, Kevin F.; Clark, Matt

    2016-12-01

    Label-free imaging of living cells below the optical diffraction limit poses great challenges for optical microscopy. Biologically relevant structural information remains below the Rayleigh limit and beyond the reach of conventional microscopes. Super-resolution techniques are typically based on the non-linear and stochastic response of fluorescent labels which can be toxic and interfere with cell function. In this paper we present, for the first time, imaging of live cells using sub-optical wavelength phonons. The axial imaging resolution of our system is determined by the acoustic wavelength (λa = λprobe/2n) and not on the NA of the optics allowing sub-optical wavelength acoustic sectioning of samples using the time of flight. The transverse resolution is currently limited to the optical spot size. The contrast mechanism is significantly determined by the mechanical properties of the cells and requires no additional contrast agent, stain or label to image the cell structure. The ability to breach the optical diffraction limit to image living cells acoustically promises to bring a new suite of imaging technologies to bear in answering exigent questions in cell biology and biomedicine.

  5. High resolution 3D imaging of living cells with sub-optical wavelength phonons

    PubMed Central

    Pérez-Cota, Fernando; Smith, Richard J.; Moradi, Emilia; Marques, Leonel; Webb, Kevin F.; Clark, Matt

    2016-01-01

    Label-free imaging of living cells below the optical diffraction limit poses great challenges for optical microscopy. Biologically relevant structural information remains below the Rayleigh limit and beyond the reach of conventional microscopes. Super-resolution techniques are typically based on the non-linear and stochastic response of fluorescent labels which can be toxic and interfere with cell function. In this paper we present, for the first time, imaging of live cells using sub-optical wavelength phonons. The axial imaging resolution of our system is determined by the acoustic wavelength (λa = λprobe/2n) and not on the NA of the optics allowing sub-optical wavelength acoustic sectioning of samples using the time of flight. The transverse resolution is currently limited to the optical spot size. The contrast mechanism is significantly determined by the mechanical properties of the cells and requires no additional contrast agent, stain or label to image the cell structure. The ability to breach the optical diffraction limit to image living cells acoustically promises to bring a new suite of imaging technologies to bear in answering exigent questions in cell biology and biomedicine. PMID:27996028

  6. Photostable and photoswitching fluorescent dyes for super-resolution imaging.

    PubMed

    Minoshima, Masafumi; Kikuchi, Kazuya

    2017-01-12

    Super-resolution fluorescence microscopy is a recently developed imaging tool for biological researches. Several methods have been developed for detection of fluorescence signals from molecules in a subdiffraction-limited area, breaking the diffraction limit of the conventional optical microscopies and allowing visualization of detailed macromolecular structures in cells. As objectives are exposed to intense laser in the optical systems, fluorophores for super-resolution microscopy must be tolerated even under severe light irradiation conditions. The fluorophores must also be photoactivatable and photoswitchable for single-molecule localization-based super-resolution microscopy, because the number of active fluorophores must be controlled by light irradiation. This has led to growing interest in these properties in the development of fluorophores. In this mini-review, we focus on the development of photostable and photoswitching fluorescent dyes for super-resolution microscopy. We introduce recent efforts, including improvement of fluorophore photostability and control of photoswitching behaviors of fluorophores based on photochemical and photophysical processes. Understanding and manipulation of chemical reactions in excited fluorophores can develop highly photostable and efficiently photoswitchable fluorophores that are suitable for super-resolution imaging applications.

  7. Super-Resolution Laser Scanning Microscopy through Spatiotemporal Modulation

    PubMed Central

    Lu, Ju; Min, Wei; Conchello, José-Angel; Xie, Xiaoliang Sunney; Lichtman, Jeff W.

    2009-01-01

    Super-resolution optical microscopy has attracted great interest among researchers in many fields, especially in biology where the scale of physical structures and molecular processes fall below the diffraction limit of resolution for light. As one of the emerging techniques, structured illumination microscopy can double the resolution by shifting unresolvable spatial frequencies into the pass-band of the microscope through spatial frequency mixing with a wide-field structured illumination pattern. However, such a wide-field scheme typically can only image optically thin samples and is incompatible with multiphoton processes such as two-photon fluorescence, which require point scanning with a focused laser beam. Here, we propose two new super-resolution schemes for laser scanning microscopy by generalizing the concept of a spatially nonuniform imaging system. One scheme, scanning patterned illumination (SPIN) microscopy, employs modulation of the excitation combined with temporally cumulative imaging by a nondescanned array detector. The other scheme, scanning patterned detection (SPADE) microscopy, utilizes detection modulation together with spatially cumulative imaging, in this case by a nondescanned single-element detector. When combined with multiphoton excitation, both schemes can image thick samples with three-dimensional optical sectioning and much improved resolution. PMID:19743870

  8. Black phosphorus photodetector for multispectral, high-resolution imaging.

    PubMed

    Engel, Michael; Steiner, Mathias; Avouris, Phaedon

    2014-11-12

    Black phosphorus is a layered semiconductor that is intensely researched in view of applications in optoelectronics. In this letter, we investigate a multilayer black phosphorus photodetector that is capable of acquiring high-contrast (V > 0.9) images both in the visible (λVIS = 532 nm) as well as in the infrared (λIR = 1550 nm) spectral regime. In a first step, by using photocurrent microscopy, we map the active area of the device and we characterize responsivity and gain. In a second step, by deploying the black phosphorus device as a point-like detector in a confocal microsope setup, we acquire diffraction-limited optical images with submicron resolution. The results demonstrate the usefulness of black phosphorus as an optoelectronic material for hyperspectral imaging applications.

  9. Conflict resolution.

    PubMed

    Levin, Roger

    2006-03-01

    The sooner conflict is identified and confronted, the more quickly it can be resolved (and the sooner, the better). When this is accomplished calmly and objectively, many areas of conflict will be eliminated. Addressing conflict as it arises also sends a clear message to the team that the practice seeks resolution, not punishment or negative consequences. In addition, the dentist and the office manager need to lead by example by avoiding gossip and encouraging open communication. The goal is to go from a parent-child relationship with the dental team to an adult-adult relationship using this series of managerial conflict resolution steps.

  10. Tracing The Origin Of Methane And Water On Mars: Mapping Regions Of Active Release At Ultra-high Spatial Resolution Using Keck And VLT Under AO Control.

    NASA Astrophysics Data System (ADS)

    Mumma, Michael J.; Villanueva, G. L.; Campbell, R.; Lyke, J.; Conrad, A.; Encrenaz, T.; Hartogh, P.; Kauefl, U.; Novak, R. E.; Tokunaga, A.

    2009-09-01

    Strong release of methane from active regions on Mars has been reported in northern summer (1) and southern spring (2). The spatial resolution of these measurements was about 500 km, sufficient to reveal discrete active regions. Regions of methane release appear mainly over ancient terrain (Noachian/Hesperian) known to have a rich hydration history, and often marked by fossae or other scarps. However, higher resolution is needed to test whether methane release is confined to a small number of narrowly defined vents or is widely distributed over the 500 km footprint. If narrowly confined, the plume should have correspondingly higher local density, enhancing spectral searches for water, methane, their isotopologues, and other trace gases that could reveal aspects of methane generation and depth of release. Ground-based telescopes equipped with both adaptive optics (AO) and high dispersion infrared spectrometers have delivered much higher spatial resolution on planetary bodies, but until now have not been applied to Mars. We acquired images and spectra of Mars under AO control at infrared wavelengths, using Keck-2 and ESO-VLT. In June 2009, we acquired test images with NIRC2/Keck-2 using AO in the natural guide star mode and achieved 0.12” FWHM resolution at 3.0 µm wavelength (Mars diameter was 4.7"). Diffraction-limited performance (0.06” at 3 µm) is expected during follow-up observations in September 2009. We observed Mars with UT1 under AO control (MACAO) in August and September 2009, and acquired spectra with CRIRES. We expect to achieve spatial resolution approaching 40 km in November-December 2009, representing a reduction in area by nearly a factor of 100 compared with earlier non-AO searches. Preliminary results will be presented. This work was funded by NASA grants 08-PAST08-0034 (Planetary Astronomy) and 08-PATM080-0031 (Planetary Atmospheres). 1. Mumma, Villanueva, Novak et al., Science 323, 1041 (2009) 2. Villanueva, Mumma, Novak, (in prep) 2009.

  11. NCAI Resolutions

    ERIC Educational Resources Information Center

    American Indian Journal of the Institute for the Development of Indian Law, 1977

    1977-01-01

    Five Major Policy Resolutions were adopted, without objection, at the 33rd Annual Convention of the National Congress of American Indians (NCAI) held in Salt Lake City, Utah, in October 1976. The issues involved were: Treaties and Trust Responsibilities, Tribal Government, Jurisdiction, Federal Administration and Structure of Indian Affairs, and…

  12. Computational high-resolution optical imaging of the living human retina

    NASA Astrophysics Data System (ADS)

    Shemonski, Nathan D.; South, Fredrick A.; Liu, Yuan-Zhi; Adie, Steven G.; Scott Carney, P.; Boppart, Stephen A.

    2015-07-01

    High-resolution in vivo imaging is of great importance for the fields of biology and medicine. The introduction of hardware-based adaptive optics (HAO) has pushed the limits of optical imaging, enabling high-resolution near diffraction-limited imaging of previously unresolvable structures. In ophthalmology, when combined with optical coherence tomography, HAO has enabled a detailed three-dimensional visualization of photoreceptor distributions and individual nerve fibre bundles in the living human retina. However, the introduction of HAO hardware and supporting software adds considerable complexity and cost to an imaging system, limiting the number of researchers and medical professionals who could benefit from the technology. Here we demonstrate a fully automated computational approach that enables high-resolution in vivo ophthalmic imaging without the need for HAO. The results demonstrate that computational methods in coherent microscopy are applicable in highly dynamic living systems.

  13. Encoding and decoding spatio-temporal information for super-resolution microscopy

    PubMed Central

    Lanzanò, Luca; Coto Hernández, Iván; Castello, Marco; Gratton, Enrico; Diaspro, Alberto; Vicidomini, Giuseppe

    2015-01-01

    The challenge of increasing the spatial resolution of an optical microscope beyond the diffraction limit can be reduced to a spectroscopy task by proper manipulation of the molecular states. The nanoscale spatial distribution of the molecules inside the detection volume of a scanning microscope can be encoded within the fluorescence dynamics and decoded by resolving the signal into its dynamics components. Here we present a robust and general method to decode this information using phasor analysis. As an example of the application of this method, we optically generate spatially controlled gradients in the fluorescence lifetime by stimulated emission. Spatial resolution can be increased indefinitely by increasing the number of resolved dynamics components up to a maximum determined by the amount of noise. We demonstrate that the proposed method provides nanoscale imaging of subcellular structures, opening new routes in super-resolution microscopy based on the encoding/decoding of spatial information through manipulation of molecular dynamics. PMID:25833391

  14. Lateral and axial resolutions of an angle-deviation microscope for different numerical apertures: experimental results

    NASA Astrophysics Data System (ADS)

    Chiu, Ming-Hung; Lai, Chin-Fa; Tan, Chen-Tai; Lin, Yi-Zhi

    2011-03-01

    This paper presents a study of the lateral and axial resolutions of a transmission laser-scanning angle-deviation microscope (TADM) with different numerical aperture (NA) values. The TADM is based on geometric optics and surface plasmon resonance principles. The surface height is proportional to the phase difference between two marginal rays of the test beam, which is passed through the test medium. We used common-path heterodyne interferometry to measure the phase difference in real time, and used a personal computer to calculate and plot the surface profile. The experimental results showed that the best lateral and axial resolutions for NA = 0.41 were 0.5 μm and 3 nm, respectively, and the lateral resolution breaks through the diffraction limits.

  15. Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states

    NASA Astrophysics Data System (ADS)

    Ehmann, Nadine; van de Linde, Sebastian; Alon, Amit; Ljaschenko, Dmitrij; Keung, Xi Zhen; Holm, Thorge; Rings, Annika; Diantonio, Aaron; Hallermann, Stefan; Ashery, Uri; Heckmann, Manfred; Sauer, Markus; Kittel, Robert J.

    2014-08-01

    The precise molecular architecture of synaptic active zones (AZs) gives rise to different structural and functional AZ states that fundamentally shape chemical neurotransmission. However, elucidating the nanoscopic protein arrangement at AZs is impeded by the diffraction-limited resolution of conventional light microscopy. Here we introduce new approaches to quantify endogenous protein organization at single-molecule resolution in situ with super-resolution imaging by direct stochastic optical reconstruction microscopy (dSTORM). Focusing on the Drosophila neuromuscular junction (NMJ), we find that the AZ cytomatrix (CAZ) is composed of units containing ~137 Bruchpilot (Brp) proteins, three quarters of which are organized into about 15 heptameric clusters. We test for a quantitative relationship between CAZ ultrastructure and neurotransmitter release properties by engaging Drosophila mutants and electrophysiology. Our results indicate that the precise nanoscopic organization of Brp distinguishes different physiological AZ states and link functional diversification to a heretofore unrecognized neuronal gradient of the CAZ ultrastructure.

  16. Encoding and decoding spatio-temporal information for super-resolution microscopy.

    PubMed

    Lanzanò, Luca; Coto Hernández, Iván; Castello, Marco; Gratton, Enrico; Diaspro, Alberto; Vicidomini, Giuseppe

    2015-04-02

    The challenge of increasing the spatial resolution of an optical microscope beyond the diffraction limit can be reduced to a spectroscopy task by proper manipulation of the molecular states. The nanoscale spatial distribution of the molecules inside the detection volume of a scanning microscope can be encoded within the fluorescence dynamics and decoded by resolving the signal into its dynamics components. Here we present a robust and general method to decode this information using phasor analysis. As an example of the application of this method, we optically generate spatially controlled gradients in the fluorescence lifetime by stimulated emission. Spatial resolution can be increased indefinitely by increasing the number of resolved dynamics components up to a maximum determined by the amount of noise. We demonstrate that the proposed method provides nanoscale imaging of subcellular structures, opening new routes in super-resolution microscopy based on the encoding/decoding of spatial information through manipulation of molecular dynamics.

  17. Nanoscale resolution in infrared imaging of protein-containing lipid membranes

    NASA Astrophysics Data System (ADS)

    Gruszecki, W. I.; Kulik, A. J.; Janik, E.; Bednarska, J.; Luchowski, R.; Grudzinski, W.; Dietler, G.

    2015-08-01

    The precise imaging of biomolecular entities contributes to an understanding of the relationship between their structure and function. However, the resolution of conventional infrared microscopic imaging is diffraction limited and does not exceed a few micrometres. Atomic force microscopy, on the other hand, can detect infrared absorption down to the sub-micrometer level. In the present report, we demonstrate that for multi-bilayer lipid samples containing the plant photosynthetic pigment-protein complex LHCII, the resolution of this latter technique can be better than 20 nm. Such a high resolution is attributable to two factors: (i) the relatively high infrared absorption by the complex that is integrated perpendicular to the plane of the multilayer film, and (ii) the distinctly different mechanical properties and thermal conductivity of the lipid and protein components of the sample.

  18. A resolution honoring the achievements of E. Thom Rumberger.

    THOMAS, 112th Congress

    Sen. Nelson, Bill [D-FL

    2011-08-01

    08/01/2011 Read twice and referred to the Committee on the Judiciary. (text of measure as introduced: CR S5196) (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  19. A resolution honoring the lifetime achievements of E. Thom Rumberger.

    THOMAS, 112th Congress

    Sen. Nelson, Bill [D-FL

    2011-09-13

    09/13/2011 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S5584-5585; text as passed Senate: CR S5585; text of measure as introduced: CR S5583) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  20. Leader as achiever.

    PubMed

    Dienemann, Jacqueline

    2002-01-01

    This article examines one outcome of leadership: productive achievement. Without achievement one is judged to not truly be a leader. Thus, the ideal leader must be a visionary, a critical thinker, an expert, a communicator, a mentor, and an achiever of organizational goals. This article explores the organizational context that supports achievement, measures of quality nursing care, fiscal accountability, leadership development, rewards and punishments, and the educational content and teaching strategies to prepare graduates to be achievers.

  1. Super-resolution imaging in optical scanning holography using structured illumination

    NASA Astrophysics Data System (ADS)

    Ren, Zhenbo; Lam, Edmund Y.

    2016-10-01

    As a specific digital holographic microscopy system, optical scanning holography (OSH) is an appealing technique that makes use of the advantages of holography in the application of optical microscopy. In OSH system, a three-dimensional object is scanned with a Fresnel zone plate in a raster fashion, and the electrical signals are demodulated into a complex hologram by heterodyne detection. Then the recorded light wavefront information contained in the hologram allows one to digitally reconstruct the specimen for multiple purposes such as optical sectioning, extended focused imaging as well as three-dimensional imaging. According to Abbe criterion, however, akin to those conventional microscopic imaging systems, OSH suffers from limited resolving power due to the finite sizes of the objective lens and the aperture, i.e., low numerical aperture. To bypass the diffraction barrier in light microscopy, various super-resolution imaging techniques have been proposed. Among those methods, structured illumination is an ensemble imaging concept that modulates the spatial frequency by projecting additional well-defined patterns with different orientation and phase shift onto the specimen. Computational algorithms are then applied to remove the effect of the structure and to reconstruct a super-resolved image beyond the diffraction-limit. In this paper, we introduce this technique in OSH system to scale down the spatial resolution beyond the diffraction limit. The performance of the proposed method is validated by simulation and experimental results.

  2. Single-Molecule Spectroscopy, Imaging, and Photocontrol: Foundations for Super-Resolution Microscopy (Nobel Lecture).

    PubMed

    Moerner, W E William E

    2015-07-06

    The initial steps toward optical detection and spectroscopy of single molecules in condensed matter arose out of the study of inhomogeneously broadened optical absorption profiles of molecular impurities in solids at low temperatures. Spectral signatures relating to the fluctuations of the number of molecules in resonance led to the attainment of the single-molecule limit in 1989 using frequency-modulation laser spectroscopy. In the early 90s, many fascinating physical effects were observed for individual molecules, and the imaging of single molecules as well as observations of spectral diffusion, optical switching and the ability to select different single molecules in the same focal volume simply by tuning the pumping laser frequency provided important forerunners of the later super-resolution microscopy with single molecules. In the room temperature regime, imaging of single copies of the green fluorescent protein also uncovered surprises, especially the blinking and photoinduced recovery of emitters, which stimulated further development of photoswitchable fluorescent protein labels. Because each single fluorophore acts a light source roughly 1 nm in size, microscopic observation and localization of individual fluorophores is a key ingredient to imaging beyond the optical diffraction limit. Combining this with active control of the number of emitting molecules in the pumped volume led to the super-resolution imaging of Eric Betzig and others, a new frontier for optical microscopy beyond the diffraction limit. The background leading up to these observations is described and current developments are summarized.

  3. Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy*

    NASA Astrophysics Data System (ADS)

    Moerner, W. E. William E.

    2015-10-01

    The initial steps toward optical detection and spectroscopy of single molecules in condensed matter arose out of the study of inhomogeneously broadened optical absorption profiles of molecular impurities in solids at low temperatures. Spectral signatures relating to the fluctuations of the number of molecules in resonance led to the attainment of the single-molecule limit in 1989 using frequency-modulation laser spectroscopy. In the early 1990s, many fascinating physical effects were observed for individual molecules, and the imaging of single molecules as well as observations of spectral diffusion, optical switching and the ability to select different single molecules in the same focal volume simply by tuning the pumping laser frequency provided important forerunners of the later super-resolution microscopy with single molecules. In the room-temperature regime, imaging of single copies of the green fluorescent protein also uncovered surprises, especially the blinking and photoinduced recovery of emitters, which stimulated further development of photoswitchable fluorescent protein labels. Because each single fluorophore acts as a light source roughly 1 nm in size, microscopic observation and localization of individual fluorophores is a key ingredient to imaging beyond the optical diffraction limit. Combining this with active control of the number of emitting molecules in the pumped volume led to the super-resolution imaging of Eric Betzig and others, a new frontier for optical microscopy beyond the diffraction limit. The background leading up to these observations is described and selected current developments are summarized.

  4. Super-resolution optical telescopes with local light diffraction shrinkage

    PubMed Central

    Wang, Changtao; Tang, Dongliang; Wang, Yanqin; Zhao, Zeyu; Wang, Jiong; Pu, Mingbo; Zhang, Yudong; Yan, Wei; Gao, Ping; Luo, Xiangang

    2015-01-01

    Suffering from giant size of objective lenses and infeasible manipulations of distant targets, telescopes could not seek helps from present super-resolution imaging, such as scanning near-field optical microscopy, perfect lens and stimulated emission depletion microscopy. In this paper, local light diffraction shrinkage associated with optical super-oscillatory phenomenon is proposed for real-time and optically restoring super-resolution imaging information in a telescope system. It is found that fine target features concealed in diffraction-limited optical images of a telescope could be observed in a small local field of view, benefiting from a relayed metasurface-based super-oscillatory imaging optics in which some local Fourier components beyond the cut-off frequency of telescope could be restored. As experimental examples, a minimal resolution to 0.55 of Rayleigh criterion is obtained, and imaging complex targets and large targets by superimposing multiple local fields of views are demonstrated as well. This investigation provides an access for real-time, incoherent and super-resolution telescopes without the manipulation of distant targets. More importantly, it gives counterintuitive evidence to the common knowledge that relayed optics could not deliver more imaging details than objective systems. PMID:26677820

  5. Super-resolution microscopy of single atoms in optical lattices

    NASA Astrophysics Data System (ADS)

    Alberti, Andrea; Robens, Carsten; Alt, Wolfgang; Brakhane, Stefan; Karski, Michał; Reimann, René; Widera, Artur; Meschede, Dieter

    2016-05-01

    We report on image processing techniques and experimental procedures to determine the lattice-site positions of single atoms in an optical lattice with high reliability, even for limited acquisition time or optical resolution. Determining the positions of atoms beyond the diffraction limit relies on parametric deconvolution in close analogy to methods employed in super-resolution microscopy. We develop a deconvolution method that makes effective use of the prior knowledge of the optical transfer function, noise properties, and discreteness of the optical lattice. We show that accurate knowledge of the image formation process enables a dramatic improvement on the localization reliability. This allows us to demonstrate super-resolution of the atoms’ position in closely packed ensembles where the separation between particles cannot be directly optically resolved. Furthermore, we demonstrate experimental methods to precisely reconstruct the point spread function with sub-pixel resolution from fluorescence images of single atoms, and we give a mathematical foundation thereof. We also discuss discretized image sampling in pixel detectors and provide a quantitative model of noise sources in electron multiplying CCD cameras. The techniques developed here are not only beneficial to neutral atom experiments, but could also be employed to improve the localization precision of trapped ions for ultra precise force sensing.

  6. Super-resolution optical telescopes with local light diffraction shrinkage.

    PubMed

    Wang, Changtao; Tang, Dongliang; Wang, Yanqin; Zhao, Zeyu; Wang, Jiong; Pu, Mingbo; Zhang, Yudong; Yan, Wei; Gao, Ping; Luo, Xiangang

    2015-12-18

    Suffering from giant size of objective lenses and infeasible manipulations of distant targets, telescopes could not seek helps from present super-resolution imaging, such as scanning near-field optical microscopy, perfect lens and stimulated emission depletion microscopy. In this paper, local light diffraction shrinkage associated with optical super-oscillatory phenomenon is proposed for real-time and optically restoring super-resolution imaging information in a telescope system. It is found that fine target features concealed in diffraction-limited optical images of a telescope could be observed in a small local field of view, benefiting from a relayed metasurface-based super-oscillatory imaging optics in which some local Fourier components beyond the cut-off frequency of telescope could be restored. As experimental examples, a minimal resolution to 0.55 of Rayleigh criterion is obtained, and imaging complex targets and large targets by superimposing multiple local fields of views are demonstrated as well. This investigation provides an access for real-time, incoherent and super-resolution telescopes without the manipulation of distant targets. More importantly, it gives counterintuitive evidence to the common knowledge that relayed optics could not deliver more imaging details than objective systems.

  7. Improving the resolution of ground-based telescopes

    NASA Astrophysics Data System (ADS)

    Roggemann, Michael C.; Welsh, Byron M.; Fugate, Robert Q.

    1997-04-01

    Atmospheric turbulence profoundly limits the angular resolution of astronomical telescopes working at visible and near-infrared wavelengths. In fact, the angular resolution for conventional imaging through turbulence is on the order of 5-20t% of the diffraction-limited resolution at the best observatories in the world. The origin of these performance degradations is random turbulence-induced fluctuations in the index of refraction of the atmosphere. Random index-of-refraction fluctuations producing the optical path length of the atmosphere to be random in both space and time, producing random aberrations in the telescope pupil that degrade imaging performance. Over the past several years significant advances have been made in developing both hardware and image-processing-based techniques for improving the resolution of astronomical telescopes. Hardware-oriented correction techniques are based on wave-front sensing and adaptive optics. Image-processing-based methods include speckle-imaging techniques and hybrid imaging techniques that use elements of adaptive-optics systems and image reconstruction. Analysis techniques for predicting the performance of these imaging methods have been developed, and the comparative performance of these imaging techniques has been examined. This paper discusses turbulence and image-detection statistics, describes the fundamentals of methods for overcoming turbulence effects, and provides representative performance results.

  8. Super-resolution optical telescopes with local light diffraction shrinkage

    NASA Astrophysics Data System (ADS)

    Wang, Changtao; Tang, Dongliang; Wang, Yanqin; Zhao, Zeyu; Wang, Jiong; Pu, Mingbo; Zhang, Yudong; Yan, Wei; Gao, Ping; Luo, Xiangang

    2015-12-01

    Suffering from giant size of objective lenses and infeasible manipulations of distant targets, telescopes could not seek helps from present super-resolution imaging, such as scanning near-field optical microscopy, perfect lens and stimulated emission depletion microscopy. In this paper, local light diffraction shrinkage associated with optical super-oscillatory phenomenon is proposed for real-time and optically restoring super-resolution imaging information in a telescope system. It is found that fine target features concealed in diffraction-limited optical images of a telescope could be observed in a small local field of view, benefiting from a relayed metasurface-based super-oscillatory imaging optics in which some local Fourier components beyond the cut-off frequency of telescope could be restored. As experimental examples, a minimal resolution to 0.55 of Rayleigh criterion is obtained, and imaging complex targets and large targets by superimposing multiple local fields of views are demonstrated as well. This investigation provides an access for real-time, incoherent and super-resolution telescopes without the manipulation of distant targets. More importantly, it gives counterintuitive evidence to the common knowledge that relayed optics could not deliver more imaging details than objective systems.

  9. High Resolution X-ray Imaging

    NASA Technical Reports Server (NTRS)

    Cash, Webster

    2002-01-01

    NAG5-5020 covered a period of 7.5 years during which a great deal of progress was made in x-ray optical techniques under this grant. We survived peer review numerous times during the effort to keep the grant going. In 1994, when the grant started we were actively pursuing the application of spherical mirrors to improving x-ray telescopes. We had found that x-ray detectors were becoming rapidly more sophisticated and affordable, but that x-ray telescopes were only being improved through the intense application of money within the AXAF program. Clearly new techniques for the future were needed. We were successful in developing and testing at the HELSTF facility in New Mexico a four reflection coma-corrected telescope made from spheres. We were able to demonstrate 0.3 arcsecond resolution, almost to the diffraction limit of the system. The community as a whole was, at that time, not particularly interested in looking past AXAF (Chandra) and the effort needed to evolve. Since we had reached the diffraction limit using non-Wolter optics we then decided to see if we could build an x-ray interferometer in the laboratory. In the lab the potential for improved resolution was substantial. If synthetic aperture telescopes could be built in space, then orders of magnitude improvement would become feasible. In 1998 NASA, under the direction of Dr Nick White of Goddard, started a study to assess the potential and feasibility of x-ray interferometry in space. My work became of central interest to the committee because it indicated that such was possible. In early 1999 we had the breakthrough that allowed us build a practical interferometer. By using flats and hooking up with the Marshall Space Flight Center facilities we were able to demonstrate fringes at 1.25keV on a one millimeter baseline. This actual laboratory demonstration provided the solid proof of concept that NASA needed. As the year progressed the future of x-ray astronomy jelled around the Maxim program. Maxim is a

  10. Subwavelength nanopatterning of photochromic diarylethene films

    SciTech Connect

    Cantu, Precious; Brimhall, Nicole; Menon, Rajesh; Andrew, Trisha L.; Castagna, Rossella; Bertarelli, Chiara

    2012-04-30

    The resolution of optical patterning is constrained by the far-field diffraction limit. In this letter, we describe an approach that exploits the unique photo- and electro-chemistry of diarylethene photochromic molecules to overcome this diffraction limit and achieve sub-wavelength nanopatterning.

  11. Fast photoacoustic imaging with a line scanning optical-acoustical resolution photoacoustic microscope (LS-OAR-PAM)

    NASA Astrophysics Data System (ADS)

    Nuster, Robert; Paltauf, Guenther

    2015-07-01

    We present the concept, the setup and a preliminary experiment using optical ultrasound detection with a CCD camera combined with focused line excitation for photoacoustic microscopy. The line scanning optical-acoustical resolution photoacoustic microscope (LS-OAR-PAM) with optical ultrasound detection is capable of real-time B-scan imaging providing acoustical resolution within the individual B-scans and optical out of plane resolution up to a depth limited by optical diffusion. A 3D image is composed of reconstructed B-scan images recorded while scanning the excitation line along the sample surface. Proof of concept is shown by imaging a phantom containing black human hairs and carbon fibers. The obtained C-scan image clearly shows the different resolution in the two perpendicular directions, namely diffraction limited by optical focusing in scan direction and acoustically limited in direction parallel to line orientation by the properties of acoustic wave propagation.

  12. High-resolution instrumentation radar

    NASA Astrophysics Data System (ADS)

    Dydbal, Robert B.; Hurlbut, Keith H.; Mori, Tsutomu T.

    1987-03-01

    An instrumentation radar that uses a chirp waveform to achieve high-range resolution is described. High-range-resolution instrumentation radars evaluate the target response to operational waveforms used in high-performance radars and/or obtain a display of the individual target scattering mechanisms to better understand the scattering process. This particular radar was efficiently constructed from a combination of commercially available components and in-house fabricated circuitry. This instrumentation radar operates at X-band and achieves a 4.9-in-range resolution. A key feature of the radar is the combination of amplitude weighting with a high degree of waveform fidelity to achieve a very good range sidelobe performance. This range sidelobe performance is important to avoid masking lower level target returns in the range sidelobes of higher target returns.

  13. Design Overview and Performance of the WIYN High Resolution Infrared Camera (WHIRC)

    NASA Astrophysics Data System (ADS)

    Meixner, Margaret; Smee, Stephen; Doering, Ryan L.; Barkhouser, Robert H.; Miller, Todd; Orndorff, Joseph; Knezek, Patricia; Churchwell, Ed; Scharfstein, Gregg; Percival, Jeffrey W.; Mills, David; Corson, Charles; Joyce, Richard R.

    2010-04-01

    We present the design overview and on-telescope performance of the WIYN High Resolution Infrared Camera (WHIRC). As a dedicated near-infrared (0.8–2.5 μm) camera on the WIYN Tip-Tilt Module (WTTM) port, WHIRC can provide near-diffraction-limited imaging with an FWHM of ∼0.25‧‧ at Ks with active WTTM correction and does deliver typical imaging with an FWHM of ∼0.6‧‧ without WTTM. WHIRC uses a 2048 × 2048 HgCdTe array from Raytheon’s VIRGO line, which has been developed for the VISTA project. The WHIRC filter complement includes J, H, Ks, and 10 narrowband filters. WHIRC’s compact design makes it the smallest near-infrared camera with this capability. We determine a gain of 3.3 ± 0.2 e- ADU-1 via a photon transfer analysis and a readout noise of ∼19 e-. A measured dark current of 0.13 e- s-1 indicates that the cryostat is extremely light tight. A plate scale of 0.099‧‧ × 0.10‧‧ pixel-1 results in a field of view (FOV) of 3.3‧ × 3.4‧, which is a compromise between the highest angular resolution achievable and the largest FOV correctable by WTTM. Measured throughput values (∼0.27 ± 0.02 in H band) are consistent with those predicted for WHIRC based on an analysis of individual optical elements and detector quantum efficiency (QE). WHIRC’s photometric quality is better than ∼0.02 magnitudes in all bands. WHIRC is a general use instrument at the WIYN telescope enabling high-definition near-infrared imaging studies of a wide range of astronomical phenomena including star formation regions, stellar populations, and interstellar medium in nearby galaxies, high- z galaxies, and transient phenomena. The WIYN Observatory is a joint facility of the University of Wisconsin-Madison, Indiana University, Yale University, and the National Optical Astronomy Observatories.

  14. Comparing Science Achievement Constructs: Targeted and Achieved

    ERIC Educational Resources Information Center

    Ferrara, Steve; Duncan, Teresa

    2011-01-01

    This article illustrates how test specifications based solely on academic content standards, without attention to other cognitive skills and item response demands, can fall short of their targeted constructs. First, the authors inductively describe the science achievement construct represented by a statewide sixth-grade science proficiency test.…

  15. Which Achievement Gap?

    ERIC Educational Resources Information Center

    Anderson, Sharon; Medrich, Elliott; Fowler, Donna

    2007-01-01

    From the halls of Congress to the local elementary school, conversations on education reform have tossed around the term "achievement gap" as though people all know precisely what that means. As it's commonly used, "achievement gap" refers to the differences in scores on state or national achievement tests between various…

  16. Multilayer Optics for Ultra-high Resolution Solar Imaging in the EUV Region

    NASA Astrophysics Data System (ADS)

    Soufli, R.; Spiller, E.; Sommargren, G. E.; Bajt, S.; Folta, J. A.; Taylor, J. S.; Gullikson, E. M.

    2003-05-01

    Highly reflective multilayer-coated optics operating at near-normal incidence angles have been the enabling technology for solar imaging instruments in the extreme ultraviolet (EUV) energy range. Despite the advances made in recent years towards understanding of solar processes through missions such as TRACE, major unresolved questions in solar physics still remain, for instance on the subjects of coronal heating, eruptive flare and coronal wind initiation. Future generations of missions will need to study the physics of hot magnetized plasmas that occur in the corona on extremely small spatial and temporal scales, requiring imaging instruments with extremely high resolution and large fields of view. Proposals for future solar missions require optics with diameters up to 700 mm and system wavefront errors as low as 0.4 nm. Experimental results will be presented for normal-incidence, four-mirror and two-mirror EUV cameras operating around 13.4 nm. Mirror substrates were manufactured by commercial vendors and achieved figure errors around 0.25 nm rms, verified by phase-shifting, point-diffraction visible light interferometers developed at Lawrence Livermore Lab. The optics were multilayer-coated aligned and tested at facilities at Lawrence Livermore and Lawrence Berkeley National Labs. A large-scale DC-magnetron sputtering tool is used to coat the optics and can accommodate multiple optics up to 600 mm in diameter in a single deposition run. During multilayer deposition, a velocity modulation algorithm is applied in order to achieve extremely precise film thickness control. The deposited Mo/Si coatings demonstrate added figure errors below 0.05 nm rms. While these systems were constructed for EUV lithographic applications, the experimental results are immediately applicable to astronomical x-ray optics. Currently these are the only multilayer-coated EUV cameras worldwide meeting such stringent specifications, and have been implemented in the construction of the first

  17. Conference Resolution

    NASA Astrophysics Data System (ADS)

    2009-04-01

    Since the first IUPAP International Conference on Women in Physics (Paris, March 2002) and the Second Conference (Rio de Janeiro, May 2005), progress has continued in most countries and world regions to attract girls to physics and advance women into leadership roles, and many working groups have formed. The Third Conference (Seoul, October 2008), with 283 attendees from 57 countries, was dedicated to celebrating the physics achievements of women throughout the world, networking toward new international collaborations, building each participant's capacity for career success, and aiding the formation of active regional working groups to advance women in physics. Despite the progress, women remain a small minority of the physics community in most countries.

  18. Maskless Plasmonic Lithography at 22 nm Resolution

    PubMed Central

    Pan, Liang; Park, Yongshik; Xiong, Yi; Ulin-Avila, Erick; Wang, Yuan; Zeng, Li; Xiong, Shaomin; Rho, Junsuk; Sun, Cheng; Bogy, David B.; Zhang, Xiang

    2011-01-01

    Optical imaging and photolithography promise broad applications in nano-electronics, metrologies, and single-molecule biology. Light diffraction however sets a fundamental limit on optical resolution, and it poses a critical challenge to the down-scaling of nano-scale manufacturing. Surface plasmons have been used to circumvent the diffraction limit as they have shorter wavelengths. However, this approach has a trade-off between resolution and energy efficiency that arises from the substantial momentum mismatch. Here we report a novel multi-stage scheme that is capable of efficiently compressing the optical energy at deep sub-wavelength scales through the progressive coupling of propagating surface plasmons (PSPs) and localized surface plasmons (LSPs). Combining this with airbearing surface technology, we demonstrate a plasmonic lithography with 22 nm half-pitch resolution at scanning speeds up to 10 m/s. This low-cost scheme has the potential of higher throughput than current photolithography, and it opens a new approach towards the next generation semiconductor manufacturing. PMID:22355690

  19. Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution

    PubMed Central

    Gustafsson, Mats G. L.

    2005-01-01

    Contrary to the well known diffraction limit, the fluorescence microscope is in principle capable of unlimited resolution. The necessary elements are spatially structured illumination light and a nonlinear dependence of the fluorescence emission rate on the illumination intensity. As an example of this concept, this article experimentally demonstrates saturated structured-illumination microscopy, a recently proposed method in which the nonlinearity arises from saturation of the excited state. This method can be used in a simple, wide-field (nonscanning) microscope, uses only a single, inexpensive laser, and requires no unusual photophysical properties of the fluorophore. The practical resolving power is determined by the signal-to-noise ratio, which in turn is limited by photobleaching. Experimental results show that a 2D point resolution of <50 nm is possible on sufficiently bright and photostable samples. PMID:16141335

  20. Simultaneous multicolor detection of RNA and proteins using super-resolution microscopy.

    PubMed

    Mito, Mari; Kawaguchi, Tetsuya; Hirose, Tetsuro; Nakagawa, Shinichi

    2016-04-01

    A number of non-membranous cellular bodies have been identified in higher eukaryotes, and these bodies contain a specific set of proteins and RNAs that are used to fulfill their functions. The size of these RNA-containing cellular bodies is usually on a submicron scale, making it difficult to observe fine structures using optical microscopy due to the diffraction limitation of visible light. Recently, microscope companies have released super-resolution microscopes that were developed using different principles, enabling the observation of sub-micron structures not resolvable in conventional fluorescent microscopy. Here, we describe multi-color fluorescent in situ hybridization techniques optimized for the simultaneous detection of RNA and proteins using super-resolution microscopy, namely structured illumination microscopy (SIM).

  1. Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution.

    PubMed

    Mo, Gary C H; Ross, Brian; Hertel, Fabian; Manna, Premashis; Yang, Xinxing; Greenwald, Eric; Booth, Chris; Plummer, Ashlee M; Tenner, Brian; Chen, Zan; Wang, Yuxiao; Kennedy, Eileen J; Cole, Philip A; Fleming, Karen G; Palmer, Amy; Jimenez, Ralph; Xiao, Jie; Dedecker, Peter; Zhang, Jin

    2017-04-01

    Compartmentalized biochemical activities are essential to all cellular processes, but there is no generalizable method to visualize dynamic protein activities in living cells at a resolution commensurate with cellular compartmentalization. Here, we introduce a new class of fluorescent biosensors that detect biochemical activities in living cells at a resolution up to threefold better than the diffraction limit. These 'FLINC' biosensors use binding-induced changes in protein fluorescence dynamics to translate kinase activities or protein-protein interactions into changes in fluorescence fluctuations, which are quantifiable through stochastic optical fluctuation imaging. A protein kinase A (PKA) biosensor allowed us to resolve minute PKA activity microdomains on the plasma membranes of living cells and to uncover the role of clustered anchoring proteins in organizing these activity microdomains. Together, these findings suggest that biochemical activities of the cell are spatially organized into an activity architecture whose structural and functional characteristics can be revealed by these new biosensors.

  2. A subwavelength resolution microwave/6.3 GHz camera based on a metamaterial absorber

    PubMed Central

    Xie, Yunsong; Fan, Xin; Chen, Yunpeng; Wilson, Jeffrey D.; Simons, Rainee N.; Xiao, John Q.

    2017-01-01

    The design, fabrication and characterization of a novel metamaterial absorber based camera with subwavelength spatial resolution are investigated. The proposed camera is featured with simple and lightweight design, easy portability, low cost, high resolution and sensitivity, and minimal image interference or distortion to the original field distribution. The imaging capability of the proposed camera was characterized in both near field and far field ranges. The experimental and simulated near field images both reveal that the camera produces qualitatively accurate images with negligible distortion to the original field distribution. The far field demonstration was done by coupling the designed camera with a microwave convex lens. The far field results further demonstrate that the camera can capture quantitatively accurate electromagnetic wave distribution in the diffraction limit. The proposed camera can be used in application such as non-destructive image and beam direction tracer. PMID:28071734

  3. Super-resolution Analysis of TCR-Dependent Signaling: Single-Molecule Localization Microscopy.

    PubMed

    Barr, Valarie A; Yi, Jason; Samelson, Lawrence E

    2017-01-01

    Single-molecule localization microscopy (SMLM) comprises methods that produce super-resolution images from molecular locations of single molecules. These techniques mathematically determine the center of a diffraction-limited spot produced by a fluorescent molecule, which represents the most likely location of the molecule. Only a small cohort of well-separated molecules is visualized in a single image, and then many images are obtained from a single sample. The localizations from all the images are combined to produce a super-resolution picture of the sample. Here we describe the application of two methods, photoactivation localization microscopy (PALM) and direct stochastic optical reconstruction microscopy (dSTORM), to the study of signaling microclusters in T cells.

  4. A subwavelength resolution microwave/6.3 GHz camera based on a metamaterial absorber

    NASA Astrophysics Data System (ADS)

    Xie, Yunsong; Fan, Xin; Chen, Yunpeng; Wilson, Jeffrey D.; Simons, Rainee N.; Xiao, John Q.

    2017-01-01

    The design, fabrication and characterization of a novel metamaterial absorber based camera with subwavelength spatial resolution are investigated. The proposed camera is featured with simple and lightweight design, easy portability, low cost, high resolution and sensitivity, and minimal image interference or distortion to the original field distribution. The imaging capability of the proposed camera was characterized in both near field and far field ranges. The experimental and simulated near field images both reveal that the camera produces qualitatively accurate images with negligible distortion to the original field distribution. The far field demonstration was done by coupling the designed camera with a microwave convex lens. The far field results further demonstrate that the camera can capture quantitatively accurate electromagnetic wave distribution in the diffraction limit. The proposed camera can be used in application such as non-destructive image and beam direction tracer.

  5. Facile method to stain the bacterial cell surface for super-resolution fluorescence microscopy

    SciTech Connect

    Gunsolus, Ian L.; Hu, Dehong; Mihai, Cosmin; Lohse, Samuel E.; Lee, Chang-Soo; Torelli, Marco; Hamers, Robert J.; Murphy, Catherine; Orr, Galya; Haynes, Christy L.

    2014-01-01

    A method to fluorescently stain the surfaces of both Gram-negative and Gram-positive bacterial cells compatible with super-resolution fluorescence microscopy is presented. This method utilizes a commercially-available fluorescent probe to label primary amines at the surface of the cell. We demonstrate efficient staining of two bacterial strains, the Gram-negative Shewanella oneidensis MR-1 and the Gram-positive Bacillus subtilis 168. Using structured illumination microscopy and stochastic optical reconstruction microscopy, which require high quantum yield or specialized dyes, we show that this staining method may be used to resolve the bacterial cell surface with sub-diffraction-limited resolution. We further use this method to identify localization patterns of nanomaterials, specifically cadmium selenide quantum dots, following interaction with bacterial cells.

  6. A subwavelength resolution microwave/6.3 GHz camera based on a metamaterial absorber.

    PubMed

    Xie, Yunsong; Fan, Xin; Chen, Yunpeng; Wilson, Jeffrey D; Simons, Rainee N; Xiao, John Q

    2017-01-10

    The design, fabrication and characterization of a novel metamaterial absorber based camera with subwavelength spatial resolution are investigated. The proposed camera is featured with simple and lightweight design, easy portability, low cost, high resolution and sensitivity, and minimal image interference or distortion to the original field distribution. The imaging capability of the proposed camera was characterized in both near field and far field ranges. The experimental and simulated near field images both reveal that the camera produces qualitatively accurate images with negligible distortion to the original field distribution. The far field demonstration was done by coupling the designed camera with a microwave convex lens. The far field results further demonstrate that the camera can capture quantitatively accurate electromagnetic wave distribution in the diffraction limit. The proposed camera can be used in application such as non-destructive image and beam direction tracer.

  7. NRL SSD Research Achievements: 19902000. Volume 4

    DTIC Science & Technology

    2015-10-30

    stratCAT. Projected future increases in computing power offer no prospect of solving this fundamental resolution constraint. Thus novel new...extraordinary ranges of research and results have been achieved. To document significant SSD historical accomplishments, Drs. George Doschek and...Howard ....................... 04 1.0 Historical Perspective

  8. Solar Confocal interferometers for Sub-Picometer-Resolution Spectral Filters

    NASA Technical Reports Server (NTRS)

    Gary, G. Allen; Pietraszewski, Chris; West, Edward A.; Dines. Terence C.

    2007-01-01

    The confocal Fabry-Perot interferometer allows sub-picometer spectral resolution of Fraunhofer line profiles. Such high spectral resolution is needed to keep pace with the higher spatial resolution of the new set of large-aperture solar telescopes. The line-of-sight spatial resolution derived for line profile inversions would then track the improvements of the transverse spatial scale provided by the larger apertures. In particular, profile inversion allows improved velocity and magnetic field gradients to be determined independent of multiple line analysis using different energy levels and ions. The confocal interferometer's unique properties allow a simultaneous increase in both etendue and spectral power. The higher throughput for the interferometer provides significant decrease in the aperture, which is important in spaceflight considerations. We have constructed and tested two confocal interferometers. A slow-response thermal-controlled interferometer provides a stable system for laboratory investigation, while a piezoelectric interferometer provides a rapid response for solar observations. In this paper we provide design parameters, show construction details, and report on the laboratory test for these interferometers. The field of view versus aperture for confocal interferometers is compared with other types of spectral imaging filters. We propose a multiple etalon system for observing with these units using existing planar interferometers as pre-filters. The radiometry for these tests established that high spectral resolution profiles can be obtained with imaging confocal interferometers. These sub-picometer spectral data of the photosphere in both the visible and near-infrared can provide important height variation information. However, at the diffraction-limited spatial resolution of the telescope, the spectral data is photon starved due to the decreased spectral passband.

  9. High-resolution instrumentation radar

    NASA Astrophysics Data System (ADS)

    Dybdal, Robert B.; Hurlbut, Keith H.; Mori, Tsutomu T.

    1986-09-01

    The development of an instrumentation radar that uses a chirp waveform to achieve high range resolution is described. Such range resolution capability is required for two reasons: (1) to evaluate the response of targets to the operational waveforms used in high-performance radars; and (2) to obtain a means of separating the individual mechanisms that comprise the target scattering response to better understand the scattering process. This particular radar was efficiently constructed from a combination of commercially available components and in-house-fabricated circuitry. This instrumentation radar operates at X-band and achieves a 4.9-in. range resolution. A key feature of the radar is its ability to combine amplitude weighting with a high degree of waveform fidelity, with the result being very good range sidelobe performance.

  10. 'No delays achiever'.

    PubMed

    2007-05-01

    The latest version of the NHS Institute for Innovation and Improvement's 'no delays achiever', a web based tool created to help NHS organisations achieve the 18-week target for GP referrals to first treatment, is available at www.nodelaysachiever.nhs.uk.

  11. Vicarious Achievement Orientation.

    ERIC Educational Resources Information Center

    Leavitt, Harold J.; And Others

    This study tests hypotheses about achievement orientation, particularly vicarious achievement. Undergraduate students (N=437) completed multiple-choice questionnaires, indicating likely responses of one person to the success of another. The sex of succeeder and observer, closeness of relationship, and setting (medical school or graduate school of…

  12. Heritability of Creative Achievement

    ERIC Educational Resources Information Center

    Piffer, Davide; Hur, Yoon-Mi

    2014-01-01

    Although creative achievement is a subject of much attention to lay people, the origin of individual differences in creative accomplishments remain poorly understood. This study examined genetic and environmental influences on creative achievement in an adult sample of 338 twins (mean age = 26.3 years; SD = 6.6 years). Twins completed the Creative…

  13. Confronting the Achievement Gap

    ERIC Educational Resources Information Center

    Gardner, David

    2007-01-01

    This article talks about the large achievement gap between children of color and their white peers. The reasons for the achievement gap are varied. First, many urban minorities come from a background of poverty. One of the detrimental effects of growing up in poverty is receiving inadequate nourishment at a time when bodies and brains are rapidly…

  14. Achievement-Based Resourcing.

    ERIC Educational Resources Information Center

    Fletcher, Mike; And Others

    1992-01-01

    This collection of seven articles examines achievement-based resourcing (ABR), the concept that the funding of educational institutions should be linked to their success in promoting student achievement, with a focus on the application of ABR to postsecondary education in the United Kingdom. The articles include: (1) "Introduction" (Mick…

  15. States Address Achievement Gaps.

    ERIC Educational Resources Information Center

    Christie, Kathy

    2002-01-01

    Summarizes 2 state initiatives to address the achievement gap: North Carolina's report by the Advisory Commission on Raising Achievement and Closing Gaps, containing an 11-point strategy, and Kentucky's legislation putting in place 10 specific processes. The North Carolina report is available at www.dpi.state.nc.us.closingthegap; Kentucky's…

  16. Fundamental limits of super-resolution microscopy by dielectric microspheres and microfibers

    NASA Astrophysics Data System (ADS)

    Astratov, V. N.; Maslov, A. V.; Allen, K. W.; Farahi, N.; Li, Y.; Brettin, A.; Limberopoulos, N. I.; Walker, D. E.; Urbas, A. M.; Liberman, V.; Rothschild, M.

    2016-03-01

    In recent years, optical super-resolution by microspheres and microfibers emerged as a new paradigm in nanoscale label-free and fluorescence imaging. However, the mechanisms of such imaging are still not completely understood and the resolution values are debated. In this work, the fundamental limits of super-resolution imaging by high-index barium-titanate microspheres and silica microfibers are studied using nanoplasmonic arrays made from Au and Al. A rigorous resolution analysis is developed based on the object's convolution with the point-spread function that has width well below the conventional (~λ/2) diffraction limit, where λ is the illumination wavelength. A resolution of ~λ/6-λ/7 is demonstrated for imaging nanoplasmonic arrays by microspheres. Similar resolution was demonstrated for microfibers in the direction perpendicular to the fiber axis with hundreds of times larger field-of-view in comparison to microspheres. Using numerical solution of Maxwell's equations, it is shown that extraordinary close point objects can be resolved in the far field, if they oscillate out of phase. Possible super-resolution using resonant excitation of whispering gallery modes is also studied.

  17. The role of molecular dipole orientation in single-molecule fluorescence microscopy and implications for super-resolution imaging.

    PubMed

    Backlund, Mikael P; Lew, Matthew D; Backer, Adam S; Sahl, Steffen J; Moerner, W E

    2014-03-17

    Numerous methods for determining the orientation of single-molecule transition dipole moments from microscopic images of the molecular fluorescence have been developed in recent years. At the same time, techniques that rely on nanometer-level accuracy in the determination of molecular position, such as single-molecule super-resolution imaging, have proven immensely successful in their ability to access unprecedented levels of detail and resolution previously hidden by the optical diffraction limit. However, the level of accuracy in the determination of position is threatened by insufficient treatment of molecular orientation. Here we review a number of methods for measuring molecular orientation using fluorescence microscopy, focusing on approaches that are most compatible with position estimation and single-molecule super-resolution imaging. We highlight recent methods based on quadrated pupil imaging and on double-helix point spread function microscopy and apply them to the study of fluorophore mobility on immunolabeled microtubules.

  18. Clean localization super-resolution microscopy for 3D biological imaging

    NASA Astrophysics Data System (ADS)

    Mondal, Partha P.; Curthoys, Nikki M.; Hess, Samuel T.

    2016-01-01

    We propose clean localization microscopy (a variant of fPALM) using a molecule filtering technique. Localization imaging involves acquiring a large number of images containing single molecule signatures followed by one-to-one mapping to render a super-resolution image. In principle, this process can be repeated for other z-planes to construct a 3D image. But, single molecules observed from off-focal planes result in false representation of their presence in the focal plane, resulting in incorrect quantification and analysis. We overcome this with a single molecule filtering technique that imposes constraints on the diffraction limited spot size of single molecules in the image plane. Calibration with sub-diffraction size beads puts a natural cutoff on the actual diffraction-limited size of single molecules in the focal plane. This helps in distinguishing beads present in the focal plane from those in the off-focal planes thereby providing an estimate of the single molecules in the focal plane. We study the distribution of actin (labeled with a photoactivatable CAGE 552 dye) in NIH 3T3 mouse fibroblast cells.

  19. Clean localization super-resolution microscopy for 3D biological imaging

    SciTech Connect

    Mondal, Partha P.; Curthoys, Nikki M.; Hess, Samuel T.

    2016-01-15

    We propose clean localization microscopy (a variant of fPALM) using a molecule filtering technique. Localization imaging involves acquiring a large number of images containing single molecule signatures followed by one-to-one mapping to render a super-resolution image. In principle, this process can be repeated for other z-planes to construct a 3D image. But, single molecules observed from off-focal planes result in false representation of their presence in the focal plane, resulting in incorrect quantification and analysis. We overcome this with a single molecule filtering technique that imposes constraints on the diffraction limited spot size of single molecules in the image plane. Calibration with sub-diffraction size beads puts a natural cutoff on the actual diffraction-limited size of single molecules in the focal plane. This helps in distinguishing beads present in the focal plane from those in the off-focal planes thereby providing an estimate of the single molecules in the focal plane. We study the distribution of actin (labeled with a photoactivatable CAGE 552 dye) in NIH 3T3 mouse fibroblast cells.

  20. High-resolution laser lithography based on vortex laser and composite layer

    NASA Astrophysics Data System (ADS)

    Zhan, Shichao; Liang, Yiyong; Li, Xiongfeng

    2016-11-01

    Traditional laser lithography systems cannot write sub-wavelength patterns due to the diffraction limit. In this paper, a novel super-resolution laser direct writing method is proposed to break through the diffraction limit. Compared with conventional lithography systems, the photoresist layer in this method is overlaid by an extra photochromic layer which is a mixture of metanil yellow and aqueous PVA solution. Then a vortex beam with a hollow energy distribution is used to expose the photochromic layer and make an annular region of the photochromic layer opaque to the writing beam. Thus, a virtual aperture is formed in the photochromic layer which can confine the diameter of the writing beam and reduce the linewidth exposed in the photoresist layer. Lithography process of this new method was modeled and a corresponding simulation was made. In this simulation, the intensity ratio of the two beams, relative absorption coefficients and other parameters were changed to study their influence to linewidth in the photoresist. An experimental setup was designed to validate the simulation, where the wavelengths of the writing beam and the vortex beam are 405 nm and 532 nm, respectively. These two beams are strictly coaxial when they are incident to the photochromic layer. The experimental results agree quite well with the model simulation, showing that the linewidth could be reduced by increasing the intensity ratio of the vortex beam to the writing beam. They also indicate that the vortex beam could effectively reduce the lithography linewidth to 300nm or even smaller.

  1. Achievability for telerobotic systems

    NASA Astrophysics Data System (ADS)

    Kress, Reid L.; Draper, John V.; Hamel, William R.

    2001-02-01

    Methods are needed to improve the capabilities of autonomous robots to perform tasks that are difficult for contemporary robots, and to identify those tasks that robots cannot perform. Additionally, in the realm of remote handling, methods are needed to assess which tasks and/or subtasks are candidates for automation. We are developing a new approach to understanding the capability of autonomous robotic systems. This approach uses formalized methods for determining the achievability of tasks for robots, that is, the likelihood that an autonomous robot or telerobot can successfully complete a particular task. Any autonomous system may be represented in achievability space by the volume describing that system's capabilities within the 3-axis space delineated by perception, cognition, and action. This volume may be thought of as a probability density with achievability decreasing as the distance from the centroid of the volume increases. Similarly, any task may be represented within achievability space. However, as tasks have more finite requirements for perception, cognition, and action, each may be represented as a point (or, more accurately, as a small sphere) within achievability space. Analysis of achievability can serve to identify, a priori, the survivability of robotic systems and the likelihood of mission success; it can be used to plan a mission or portions of a mission; it can be used to modify a mission plan to accommodate unpredicted occurrences; it can also serve to identify needs for modifications to robotic systems or tasks to improve achievability. .

  2. Resolution enhancement techniques in microscopy

    NASA Astrophysics Data System (ADS)

    Cremer, Christoph; Masters, Barry R.

    2013-05-01

    We survey the history of resolution enhancement techniques in microscopy and their impact on current research in biomedicine. Often these techniques are labeled superresolution, or enhanced resolution microscopy, or light-optical nanoscopy. First, we introduce the development of diffraction theory in its relation to enhanced resolution; then we explore the foundations of resolution as expounded by the astronomers and the physicists and describe the conditions for which they apply. Then we elucidate Ernst Abbe's theory of optical formation in the microscope, and its experimental verification and dissemination to the world wide microscope communities. Second, we describe and compare the early techniques that can enhance the resolution of the microscope. Third, we present the historical development of various techniques that substantially enhance the optical resolution of the light microscope. These enhanced resolution techniques in their modern form constitute an active area of research with seminal applications in biology and medicine. Our historical survey of the field of resolution enhancement uncovers many examples of reinvention, rediscovery, and independent invention and development of similar proposals, concepts, techniques, and instruments. Attribution of credit is therefore confounded by the fact that for understandable reasons authors stress the achievements from their own research groups and sometimes obfuscate their contributions and the prior art of others. In some cases, attribution of credit is also made more complex by the fact that long term developments are difficult to allocate to a specific individual because of the many mutual connections often existing between sometimes fiercely competing, sometimes strongly collaborating groups. Since applications in biology and medicine have been a major driving force in the development of resolution enhancing approaches, we focus on the contribution of enhanced resolution to these fields.

  3. Optical resolution of rotenoids

    USGS Publications Warehouse

    Abidi, S.L.

    1987-01-01

    Optical resolution of selected rotenoids containing 1-3 asymmetric centers in dihydrobenzopyranofuroben-zopyranone and dihydrobisbenzopyranopyranone series has been achieved on two chiral high-performance liquid chromatographic (hplc) stationary phases. In most cases, the absolute stereochemistry at the cis-B/C ring junction of the rotenoidal antipodes can be related to their elution order. Generally, the 6aα,12aα-enantiomers were more strongly retained by the chiral substrate than their corresponding optical antipodes. The elution-configuration relationship provides potential utility for predicting the absolute configuration of related rotenoidal compounds. Chiral phase hplc on amino-acid-bonded-silica yielded results explicable in terms of Pirkle's bonding schemes for chiral recognition. Resolution data for 12a-hydroxy-, 12a-methoxy-, and 12-hydroxyiminorotenoids further corroborate the mechanistic rationale, and demonstrate that nonpolar π-π interactions appeared to be important for enantiomeric separation on helic poly-triphenylmethylacryl-ate-silica (CPOT). In the latter system, steric effects and conformational factors in association with the modification of E-ring structures might play significant roles in the chiral separation process in view of the reversal to the elution order observed for all methoxylated rotenoids and elliptone derivatives including the parent deguelin. The unique separability (α = 1.44) of 12a-hydroxyelliptone on CPOT was suggestive of structural effects of the 5-side chain on the resolution of the rotenoids having a five-membered-E-ring. The results obtained with two different types of chiral phases are complementary and useful for optical resolution of a wide variety of natural and synthetic rotenoidal compounds.

  4. Culture and Achievement Motivation

    ERIC Educational Resources Information Center

    Maehr, Martin L.

    1974-01-01

    A framework is suggested for the cross-cultural study of motivation that stresses the importance of contextual conditions in eliciting achievement motivation and emphasizes cultural relativity in the definition of the concept. (EH)

  5. Achieving Salary Equity

    ERIC Educational Resources Information Center

    Nevill, Dorothy D.

    1975-01-01

    Three techniques are outlined for use by higher education institutions to achieve salary equity: salary prediction (using various statistical procedures), counterparting (comparing salaries of persons of similar rank), and grievance procedures. (JT)

  6. Super-resolution imaging of neuronal dense-core vesicles.

    PubMed

    Scalettar, Bethe A; Shaver, Daniel; Kaech, Stefanie; Lochner, Janis E

    2014-07-02

    Detection of fluorescence provides the foundation for many widely utilized and rapidly advancing microscopy techniques employed in modern biological and medical applications. Strengths of fluorescence include its sensitivity, specificity, and compatibility with live imaging. Unfortunately, conventional forms of fluorescence microscopy suffer from one major weakness, diffraction-limited resolution in the imaging plane, which hampers studies of structures with dimensions smaller than ~250 nm. Recently, this limitation has been overcome with the introduction of super-resolution fluorescence microscopy techniques, such as photoactivated localization microscopy (PALM). Unlike its conventional counterparts, PALM can produce images with a lateral resolution of tens of nanometers. It is thus now possible to use fluorescence, with its myriad strengths, to elucidate a spectrum of previously inaccessible attributes of cellular structure and organization. Unfortunately, PALM is not trivial to implement, and successful strategies often must be tailored to the type of system under study. In this article, we show how to implement single-color PALM studies of vesicular structures in fixed, cultured neurons. PALM is ideally suited to the study of vesicles, which have dimensions that typically range from ~50-250 nm. Key steps in our approach include labeling neurons with photoconvertible (green to red) chimeras of vesicle cargo, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a high-resolution PALM image. We also demonstrate the efficacy of our approach by presenting exceptionally well-resolved images of dense-core vesicles (DCVs) in cultured hippocampal neurons, which refute the hypothesis that extrasynaptic trafficking of DCVs is mediated largely by DCV clusters.

  7. Performance Characterization of a Switchable Acoustic Resolution and Optical Resolution Photoacoustic Microscopy System.

    PubMed

    Moothanchery, Mohesh; Pramanik, Manojit

    2017-02-12

    Photoacoustic microscopy (PAM) is a scalable bioimaging modality; one can choose low acoustic resolution with deep penetration depth or high optical resolution with shallow imaging depth. High spatial resolution and deep penetration depth is rather difficult to achieve using a single system. Here we report a switchable acoustic resolution and optical resolution photoacoustic microscopy (AR-OR-PAM) system in a single imaging system capable of both high resolution and low resolution on the same sample. Lateral resolution of 4.2 µm (with ~1.4 mm imaging depth) and lateral resolution of 45 μm (with ~7.6 mm imaging depth) was successfully demonstrated using a switchable system. In vivo blood vasculature imaging was also performed for its biological application.

  8. Performance Characterization of a Switchable Acoustic Resolution and Optical Resolution Photoacoustic Microscopy System

    PubMed Central

    Moothanchery, Mohesh; Pramanik, Manojit

    2017-01-01

    Photoacoustic microscopy (PAM) is a scalable bioimaging modality; one can choose low acoustic resolution with deep penetration depth or high optical resolution with shallow imaging depth. High spatial resolution and deep penetration depth is rather difficult to achieve using a single system. Here we report a switchable acoustic resolution and optical resolution photoacoustic microscopy (AR-OR-PAM) system in a single imaging system capable of both high resolution and low resolution on the same sample. Lateral resolution of 4.2 µm (with ~1.4 mm imaging depth) and lateral resolution of 45 μm (with ~7.6 mm imaging depth) was successfully demonstrated using a switchable system. In vivo blood vasculature imaging was also performed for its biological application. PMID:28208676

  9. Evaluation of Advanced Bionics high resolution mode.

    PubMed

    Buechner, Andreas; Frohne-Buechner, Carolin; Gaertner, Lutz; Lesinski-Schiedat, Anke; Battmer, Rolf-Dieter; Lenarz, Thomas

    2006-07-01

    The objective of this paper is to evaluate the advantages of the Advanced Bionic high resolution mode for speech perception, through a retrospective analysis. Forty-five adult subjects were selected who had a minimum experience of three months' standard mode (mean of 10 months) before switching to high resolution mode. Speech perception was tested in standard mode immediately before fitting with high resolution mode, and again after a maximum of six months high resolution mode usage (mean of two months). A significant improvement was found, between 11 and 17%, depending on the test material. The standard mode preference does not give any indication about the improvement when switching to high resolution. Users who are converted within any study achieve a higher performance improvement than those converted in the clinical routine. This analysis proves the significant benefits of high resolution mode for users, and also indicates the need for guidelines for individual optimization of parameter settings in a high resolution mode program.

  10. Refocusing capabilities in a miniaturized multi-channel multi-resolution imaging system using a tunable lens

    NASA Astrophysics Data System (ADS)

    Smeesters, L.; Belay, Gebirie Y.; Ottevaere, H.; Meuret, Youri; Thienpont, H.

    2014-05-01

    Inspired by nature, many application domains might gain from combining the multi-channel design of the compound eyes of insects and the refocusing capability of the human eye in one compact configuration. Multi-channel refocusing imaging systems are nowadays only commercially available in bulky and expensive designs since classical refocusing mechanisms cannot be integrated in a miniaturized configuration. We designed a wafer-level multi-resolution two-channel imaging system with refocusing capabilities using a voltage tunable liquid lens. One channel is able to capture a wide field-of-view image (2x40°) of a surrounding with a low angular resolution (0.078°), whereas a detailed image of a small region of interest (2x7.57°) can be obtained with the high angular resolution channel (0.0098°). The latter high angular resolution channel contains the tunable lens and therefore also the refocusing capabilities. In this paper, we first discuss the working principle, tunability and optical quality of a voltage tunable liquid lens. Based on optical characterization measurements with a Mach-Zehnder interferometer, we designed a tunable lens model. The designed tunable lens model and its validation in an imaging setup show a diffraction-limited image quality. Following, we discuss the performance of the designed two-channel imaging system. Both the wide field-of-view and high angular resolution optical channels show a diffraction-limited performance, ensuring a good image quality. Moreover, we obtained an improved depth-of-field, from 0.254m until infinity, in comparison with the current state-of-the art published wafer-level multi-channel imaging systems, which show a depth-of-field from 9m until infinity.

  11. Resolution in Electromagnetic Prospecting

    NASA Astrophysics Data System (ADS)

    Aldridge, D. F.; Bartel, L. C.; Knox, H. A.; Schramm, K. A.

    2014-12-01

    Low-frequency electromagnetic (EM) signals are commonly used in geophysical exploration of the shallow subsurface. Sensitivity to conductivity implies they are particularly useful for inferring fluid content of porous media. However, low-frequency EM wavefields are diffusive, and have significantly larger wavelengths compared to seismic signals of equal frequency. The wavelength of a 30 Hz sinusoid propagating with seismic velocity 3000 m/s is 100 m, whereas an analogous EM signal diffusing through a conductive body of 0.1 S/m (clayey shale) has wavelength 1825 m. The larger wavelength has implications for resolution of the EM prospecting method. We are investigating resolving power of the EM method via theoretical and numerical experiments. Normal incidence plane wave reflection/transmission by a thin geologic bed is amenable to analytic solution. Responses are calculated for beds that are conductive or resistive relative to the host rock. Preliminary results indicate the classic seismic resolution/detection limit of bed thickness ~1/8 wavelength is not achieved. EM responses for point or line current sources recorded by general acquisition geometries are calculated with a 3D finite-difference algorithm. These exhibit greater variability which may allow inference of bed thickness. We also examine composite responses of two point scatterers with separation when illuminated by an incident EM field. This is analogous to the Rayleigh resolution problem of estimating angular separation between two light sources. The First Born Approximation implies that perturbations in permittivity, permeability, and conductivity have different scattering patterns, which may be indicators of EM medium properties. Sandia National Laboratories is a multi-program laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the US Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000.

  12. SALT and Spelling Achievement.

    ERIC Educational Resources Information Center

    Nelson, Joan

    A study investigated the effects of suggestopedic accelerative learning and teaching (SALT) on the spelling achievement, attitudes toward school, and memory skills of fourth-grade students. Subjects were 20 male and 28 female students from two self-contained classrooms at Kennedy Elementary School in Rexburg, Idaho. The control classroom and the…

  13. Iowa Women of Achievement.

    ERIC Educational Resources Information Center

    Ohrn, Deborah Gore, Ed.

    1993-01-01

    This issue of the Goldfinch highlights some of Iowa's 20th century women of achievement. These women have devoted their lives to working for human rights, education, equality, and individual rights. They come from the worlds of politics, art, music, education, sports, business, entertainment, and social work. They represent Native Americans,…

  14. Schools Achieving Gender Equity.

    ERIC Educational Resources Information Center

    Revis, Emma

    This guide is designed to assist teachers presenting the Schools Achieving Gender Equity (SAGE) curriculum for vocational education students, which was developed to align gender equity concepts with the Kentucky Education Reform Act (KERA). Included in the guide are lesson plans for classes on the following topics: legal issues of gender equity,…

  15. Achieving Peace through Education.

    ERIC Educational Resources Information Center

    Clarken, Rodney H.

    While it is generally agreed that peace is desirable, there are barriers to achieving a peaceful world. These barriers are classified into three major areas: (1) an erroneous view of human nature; (2) injustice; and (3) fear of world unity. In a discussion of these barriers, it is noted that although the consciousness and conscience of the world…

  16. Explorations in achievement motivation

    NASA Technical Reports Server (NTRS)

    Helmreich, Robert L.

    1982-01-01

    Recent research on the nature of achievement motivation is reviewed. A three-factor model of intrinsic motives is presented and related to various criteria of performance, job satisfaction and leisure activities. The relationships between intrinsic and extrinsic motives are discussed. Needed areas for future research are described.

  17. Increasing Male Academic Achievement

    ERIC Educational Resources Information Center

    Jackson, Barbara Talbert

    2008-01-01

    The No Child Left Behind legislation has brought greater attention to the academic performance of American youth. Its emphasis on student achievement requires a closer analysis of assessment data by school districts. To address the findings, educators must seek strategies to remedy failing results. In a mid-Atlantic district of the Unites States,…

  18. Appraising Reading Achievement.

    ERIC Educational Resources Information Center

    Ediger, Marlow

    To determine quality sequence in pupil progress, evaluation approaches need to be used which guide the teacher to assist learners to attain optimally. Teachers must use a variety of procedures to appraise student achievement in reading, because no one approach is adequate. Appraisal approaches might include: (1) observation and subsequent…

  19. Cognitive Processes and Achievement.

    ERIC Educational Resources Information Center

    Hunt, Dennis; Randhawa, Bikkar S.

    For a group of 165 fourth- and fifth-grade students, four achievement test scores were correlated with success on nine tests designed to measure three cognitive functions: sustained attention, successive processing, and simultaneous processing. This experiment was designed in accordance with Luria's model of the three functional units of the…

  20. Graders' Mathematics Achievement

    ERIC Educational Resources Information Center

    Bond, John B.; Ellis, Arthur K.

    2013-01-01

    The purpose of this experimental study was to investigate the effects of metacognitive reflective assessment instruction on student achievement in mathematics. The study compared the performance of 141 students who practiced reflective assessment strategies with students who did not. A posttest-only control group design was employed, and results…

  1. Achieving All Our Ambitions

    ERIC Educational Resources Information Center

    Hartley, Tricia

    2009-01-01

    National learning and skills policy aims both to build economic prosperity and to achieve social justice. Participation in higher education (HE) has the potential to contribute substantially to both aims. That is why the Campaign for Learning has supported the ambition to increase the proportion of the working-age population with a Level 4…

  2. Improving Educational Achievement.

    ERIC Educational Resources Information Center

    New York University Education Quarterly, 1979

    1979-01-01

    This is a slightly abridged version of the report of the National Academy of Education panel, convened at the request of HEW Secretary Joseph Califano and Assistant Secretary for Education Mary F. Berry, to study recent declines in student achievement and methods of educational improvement. (SJL)

  3. The Achievement Club

    ERIC Educational Resources Information Center

    Rogers, Ibram

    2009-01-01

    When Gabrielle Carpenter became a guidance counselor in Northern Virginia nine years ago, she focused on the academic achievement gap and furiously tried to close it. At first, she was compelled by tremendous professional interest. However, after seeing her son lose his zeal for school, Carpenter joined forces with other parents to form an…

  4. Achievement in Problem Solving

    ERIC Educational Resources Information Center

    Friebele, David

    2010-01-01

    This Action Research Project is meant to investigate the effects of incorporating research-based instructional strategies into instruction and their subsequent effect on student achievement in the area of problem-solving. The two specific strategies utilized are the integration of manipulatives and increased social interaction on a regular basis.…

  5. Essays on Educational Achievement

    ERIC Educational Resources Information Center

    Ampaabeng, Samuel Kofi

    2013-01-01

    This dissertation examines the determinants of student outcomes--achievement, attainment, occupational choices and earnings--in three different contexts. The first two chapters focus on Ghana while the final chapter focuses on the US state of Massachusetts. In the first chapter, I exploit the incidence of famine and malnutrition that resulted to…

  6. Advancing Student Achievement

    ERIC Educational Resources Information Center

    Walberg, Herbert J.

    2010-01-01

    For the last half century, higher spending and many modern reforms have failed to raise the achievement of students in the United States to the levels of other economically advanced countries. A possible explanation, says Herbert Walberg, is that much current education theory is ill informed about scientific psychology, often drawing on fads and…

  7. NCLB: Achievement Robin Hood?

    ERIC Educational Resources Information Center

    Bracey, Gerald W.

    2008-01-01

    In his "Wall Street Journal" op-ed on the 25th of anniversary of "A Nation At Risk", former assistant secretary of education Chester E. Finn Jr. applauded the report for turning U.S. education away from equality and toward achievement. It was not surprising, then, that in mid-2008, Finn arranged a conference to examine the…

  8. Interference microscopy: Super-resolution particle tracking and velocimetry

    NASA Astrophysics Data System (ADS)

    Snoeyink, Craig A.

    measurements. A clear increase in resolution is found, nearly double that of the base microscope. The last chapter discusses the ability of the IM system to resolve features that are smaller than the diffraction limit of the base microscope. The Reyleigh limit for the IM system is shown to be less than one third the base microscope. Diffraction simulations are performed to verify this limit and experimental images of sub-diffraction limit particles are presented.

  9. Interactively variable isotropic resolution in computed tomography.

    PubMed

    Lapp, Robert M; Kyriakou, Yiannis; Kachelriess, Marc; Wilharm, Sylvia; Kalender, Willi A

    2008-05-21

    An individual balancing between spatial resolution and image noise is necessary to fulfil the diagnostic requirements in medical CT imaging. In order to change influencing parameters, such as reconstruction kernel or effective slice thickness, additional raw-data-dependent image reconstructions have to be performed. Therefore, the noise versus resolution trade-off is time consuming and not interactively applicable. Furthermore, isotropic resolution, expressed by an equivalent point spread function (PSF) in every spatial direction, is important for the undistorted visualization and quantitative evaluation of small structures independent of the viewing plane. Theoretically, isotropic resolution can be obtained by matching the in-plane and through-plane resolution with the aforementioned parameters. Practically, however, the user is not assisted in doing so by current reconstruction systems and therefore isotropic resolution is not commonly achieved, in particular not at the desired resolution level. In this paper, an integrated approach is presented for equalizing the in-plane and through-plane spatial resolution by image filtering. The required filter kernels are calculated from previously measured PSFs in x/y- and z-direction. The concepts derived are combined with a variable resolution filtering technique. Both approaches are independent of CT raw data and operate only on reconstructed images which allows for their application in real time. Thereby, the aim of interactively variable, isotropic resolution is achieved. Results were evaluated quantitatively by measuring PSFs and image noise, and qualitatively by comparing the images to direct reconstructions regarded as the gold standard. Filtered images matched direct reconstructions with arbitrary reconstruction kernels with standard deviations in difference images of typically between 1 and 17 HU. Isotropic resolution was achieved within 5% of the selected resolution level. Processing times of 20-100 ms per frame

  10. Resolution enhancement in tilted coordinates

    NASA Astrophysics Data System (ADS)

    Hariri Naghadeh, Diako; Keith Morley, Christopher

    2016-11-01

    Deconvolution is applied to remove source wavelet effects from seismograms. The results are resolution enhancement that enables detection of thin layers. Following enhancement of resolution, low frequency and high angle reflectors, particularly at great depth, appear as low amplitude and semi-invisible reflectors that are difficult to track and pick. A new approach to enhance resolution is introduced that estimates a derivative using continuous wavelet transform in tilted coordinates. The results are compared with sparse spike deconvolution, curvelet deconvolution and inverse quality filtering in wavelet domain. The positive consequence of the new method is to increase sampling of high dip features by changing the coordinate system from Cartesian to tilted. To compare those methods a complex data set was chosen that includes high angle faults and chaotic mass transport complex. Image enhancement using curvelet deconvolution shows a chaotic system as a non-chaotic one. The results show that sparse spike deconvolution and inverse quality filtering in wavelet domain are able to enhance resolution more than curvelet deconvolution especially at great depth but it is impossible to follow steep dip reflectors after resolution enhancement using these methods, especially when their apparent dips are more than 45°. By estimating derivatives in a continuous wavelet transform from tilted data sets similar resolution enhancement as the other deconvolution methods is achieved but additionally steep dipping reflectors are imaged much better than others. Subtracted results of the enhanced resolution data set using new method and the other introduced methods show that steeply dipping reflectors are highlighted as a particular ability of the new method. The results show that high frequency recovery in Cartesian co-ordinate is accompanied by inability to image steeply dipping reflectors especially at great depths. Conversely recovery of high frequency data and imaging of the data

  11. Fundamental Limits of Spatial Resolution in PET

    PubMed Central

    Moses, William W.

    2010-01-01

    The fundamental limits of spatial resolution in positron emission tomography (PET) have been understood for many years. The physical size of the detector element usually plays the dominant role in determining resolution, but the combined contributions from acollinearity, positron range, penetration into the detector ring, and decoding errors in the detector modules often combine to be of similar size. In addition, the sampling geometry and statistical noise further degrade the effective resolution. This paper describes quantitatively describes these effects, discusses potential methods for reducing the magnitude of these effects, and computes the ultimately achievable spatial resolution for clinical and pre-clinical PET cameras. PMID:21804677

  12. KINOFORM LENSES - TOWARD NANOMETER RESOLUTION.

    SciTech Connect

    STEIN, A.; EVANS-LUTTERODT, K.; TAYLOR, A.

    2004-10-23

    While hard x-rays have wavelengths in the nanometer and sub-nanometer range, the ability to focus them is limited by the quality of sources and optics, and not by the wavelength. A few options, including reflective (mirrors), diffractive (zone plates) and refractive (CRL's) are available, each with their own limitations. Here we present our work with kinoform lenses which are refractive lenses with all material causing redundant 2{pi} phase shifts removed to reduce the absorption problems inherently limiting the resolution of refractive lenses. By stacking kinoform lenses together, the effective numerical aperture, and thus the focusing resolution, can be increased. The present status of kinoform lens fabrication and testing at Brookhaven is presented as well as future plans toward achieving nanometer resolution.

  13. High resolution optical DNA mapping

    NASA Astrophysics Data System (ADS)

    Baday, Murat

    Many types of diseases including cancer and autism are associated with copy-number variations in the genome. Most of these variations could not be identified with existing sequencing and optical DNA mapping methods. We have developed Multi-color Super-resolution technique, with potential for high throughput and low cost, which can allow us to recognize more of these variations. Our technique has made 10--fold improvement in the resolution of optical DNA mapping. Using a 180 kb BAC clone as a model system, we resolved dense patterns from 108 fluorescent labels of two different colors representing two different sequence-motifs. Overall, a detailed DNA map with 100 bp resolution was achieved, which has the potential to reveal detailed information about genetic variance and to facilitate medical diagnosis of genetic disease.

  14. Faculty achievement tracking tool.

    PubMed

    Pettus, Sarah; Reifschneider, Ellen; Burruss, Nancy

    2009-03-01

    Faculty development and scholarship is an expectation of nurse educators. Accrediting institutions, such as the Commission on Collegiate Nursing Education, the National League for Nursing Accrediting Commission, and the Higher Learning Commission, all have criteria regarding faculty achievement. A faculty achievement tracking tool (FATT) was developed to facilitate documentation of accreditation criteria attainment. Based on criteria from accrediting organizations, the roles that are addressed include scholarship, service, and practice. Definitions and benchmarks for the faculty as an aggregate are included. Undergoing reviews from different accrediting organizations, the FATT has been used once for accreditation of the undergraduate program and once for accreditation of the graduate program. The FATT is easy to use and has become an excellent adjunct for the preparation for accreditation reports. In addition, the FATT may be used for yearly evaluations, advancement, and merit.

  15. Project ACHIEVE final report

    SciTech Connect

    1997-06-13

    Project ACHIEVE was a math/science academic enhancement program aimed at first year high school Hispanic American students. Four high schools -- two in El Paso, Texas and two in Bakersfield, California -- participated in this Department of Energy-funded program during the spring and summer of 1996. Over 50 students, many of whom felt they were facing a nightmare future, were given the opportunity to work closely with personal computers and software, sophisticated calculators, and computer-based laboratories -- an experience which their regular academic curriculum did not provide. Math and science projects, exercises, and experiments were completed that emphasized independent and creative applications of scientific and mathematical theories to real world problems. The most important outcome was the exposure Project ACHIEVE provided to students concerning the college and technical-field career possibilities available to them.

  16. Single objective light-sheet microscopy for high-speed whole-cell 3D super-resolution

    PubMed Central

    Meddens, Marjolein B. M.; Liu, Sheng; Finnegan, Patrick S.; Edwards, Thayne L.; James, Conrad D.; Lidke, Keith A.

    2016-01-01

    We have developed a method for performing light-sheet microscopy with a single high numerical aperture lens by integrating reflective side walls into a microfluidic chip. These 45° side walls generate light-sheet illumination by reflecting a vertical light-sheet into the focal plane of the objective. Light-sheet illumination of cells loaded in the channels increases image quality in diffraction limited imaging via reduction of out-of-focus background light. Single molecule super-resolution is also improved by the decreased background resulting in better localization precision and decreased photo-bleaching, leading to more accepted localizations overall and higher quality images. Moreover, 2D and 3D single molecule super-resolution data can be acquired faster by taking advantage of the increased illumination intensities as compared to wide field, in the focused light-sheet. PMID:27375939

  17. Single objective light-sheet microscopy for high-speed whole-cell 3D super-resolution.

    PubMed

    Meddens, Marjolein B M; Liu, Sheng; Finnegan, Patrick S; Edwards, Thayne L; James, Conrad D; Lidke, Keith A

    2016-06-01

    We have developed a method for performing light-sheet microscopy with a single high numerical aperture lens by integrating reflective side walls into a microfluidic chip. These 45° side walls generate light-sheet illumination by reflecting a vertical light-sheet into the focal plane of the objective. Light-sheet illumination of cells loaded in the channels increases image quality in diffraction limited imaging via reduction of out-of-focus background light. Single molecule super-resolution is also improved by the decreased background resulting in better localization precision and decreased photo-bleaching, leading to more accepted localizations overall and higher quality images. Moreover, 2D and 3D single molecule super-resolution data can be acquired faster by taking advantage of the increased illumination intensities as compared to wide field, in the focused light-sheet.

  18. Does super-resolution fluorescence microscopy obsolete previous microscopic approaches to protein co-localization?

    PubMed

    MacDonald, Laura; Baldini, Giulia; Storrie, Brian

    2015-01-01

    Conventional microscopy techniques, namely, the confocal microscope or deconvolution processes, are resolution limited to approximately 200-250 nm by the diffraction properties of light as developed by Ernst Abbe in 1873. This diffraction limit is appreciably above the size of most multi-protein complexes, which are typically 20-50 nm in diameter. In the mid-2000s, biophysicists moved beyond the diffraction barrier by structuring the illumination pattern and then applying mathematical principles and algorithms to allow a resolution of approximately 100 nm, sufficient to address protein subcellular co-localization questions. This "breaking" of the diffraction barrier, affording resolution beyond 200 nm, is termed super-resolution microscopy. More recent approaches include single-molecule localization (such as photoactivated localization microscopy (PALM)/stochastic optical reconstruction microscopy (STORM)) and point spread function engineering (such as stimulated emission depletion (STED) microscopy). In this review, we explain basic principles behind currently commercialized super-resolution setups and address advantages and considerations in applying these techniques to protein co-localization in biological systems.

  19. STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.

    PubMed

    Ilgen, Peter; Stoldt, Stefan; Conradi, Lena-Christin; Wurm, Christian Andreas; Rüschoff, Josef; Ghadimi, B Michael; Liersch, Torsten; Jakobs, Stefan

    2014-01-01

    Formalin fixed and paraffin-embedded human tissue resected during cancer surgery is indispensable for diagnostic and therapeutic purposes and represents a vast and largely unexploited resource for research. Optical microscopy of such specimen is curtailed by the diffraction-limited resolution of conventional optical microscopy. To overcome this limitation, we used STED super-resolution microscopy enabling optical resolution well below the diffraction barrier. We visualized nanoscale protein distributions in sections of well-annotated paraffin-embedded human rectal cancer tissue stored in a clinical repository. Using antisera against several mitochondrial proteins, STED microscopy revealed distinct sub-mitochondrial protein distributions, suggesting a high level of structural preservation. Analysis of human tissues stored for up to 17 years demonstrated that these samples were still amenable for super-resolution microscopy. STED microscopy of sections of HER2 positive rectal adenocarcinoma revealed details in the surface and intracellular HER2 distribution that were blurred in the corresponding conventional images, demonstrating the potential of super-resolution microscopy to explore the thus far largely untapped nanoscale regime in tissues stored in biorepositories.

  20. Design of an eight-element refractive lens for high resolution imaging applications

    NASA Astrophysics Data System (ADS)

    Rao, D. V. B.

    1987-06-01

    An 8 element refractive lens of 324.4 mm EFL and f/4.5 was designed for the spectral band 520nm to 590nm. The FOV of the lens was +- 5 degrees. The computed MTF of this lens system was better than 0.7 for spatial frequencies up to 60 1p/mm, as against the diffraction limited MTF of 0.81. A similar lens was designed for the spectral band 770nm to 860nm. These two lenses will be configured alongwith a panchromatic catadioptric lens of 900 mm EFL for high resolution spacecraft remote sensing applications. The design details of these lenses were presented in this paper.

  1. Super resolution nano-information recording in a new hydrazone metal complex material

    NASA Astrophysics Data System (ADS)

    Zhang, Kui; Wei, Jingsong; Chen, Zhimin; Wei, Tao; Geng, Yongyou; Wang, Yang; Wu, Yiqun

    2016-10-01

    Laser thermal lithography has been proposed for a few years, which has the advantages of breaking through the optical diffraction limit, operation in far-field and in air, and low production cost. In this paper, a new hydrazone metal complex is used as the laser thermal lithography material due to its feature of the one-step fabrication of micro/nano structure without mask and wet-etching process. Based on the laser thermal lithography method, super resolution nano-information pits are directly written on the surface of hydrazone metal complex thin films. Pits with a minimum feature size of about 79 nm are successfully obtained, which is only about 1/7 of the writing spot size. Moreover, the reactive ion etching method can be applied to transfer the pits onto a silica substrate. These results suggest the potential applications of the new material in high density optical data storage and semiconductor industries.

  2. High Resolution Resonant Inelastic Light Scattering from 185 to 900 nm

    NASA Astrophysics Data System (ADS)

    Rubhausen, Michael; Backstrom, Joakim; Schulz, Benjamin; Kruger, Rilana; Budelmann, Dirk

    2003-03-01

    We present the concept, design, and first results of a spectrally very agile Raman spectrometer that has a fully achromatic and parabolic focussing on the sample and an achromatic coupling into a spectrometer that operates in a wavelength range between 185 and 900 nm. Due to the diffraction limited imaging it features very high spectral and spatial resolution as well as excellent efficiency. A special spatial filter stage between the two subtractively coupled premonochromators allows very high straylight rejection even in the deep ultraviolett spectral range. This filter stage is also common to all systems previously developed in the 'ultimative triple' series by Miles Klein (Urbana-Champaign) and is shown here to be crucial for measurements in this difficult spectral range. First results of resonance Raman measurements on Silicon, LaMnO3 and the Keratin matrix are shown and compared with theoretical expectations.

  3. High spatial resolution confocal microscope with independent excitation and detection scanning capabilities.

    PubMed

    Marcet, S; Ouellet-Plamondon, C; Francoeur, S

    2009-06-01

    We present the design of a confocal microscope adapted for optical spectroscopy and imaging at cryogenic temperatures. This system is based on the existing approach of partly inserting the optical components of the microscope inside a helium-bath cryostat. It provides a spatial resolution approaching the diffraction limit with a mechanical stability allowing uninterrupted integration times exceeding 10 h and allows keeping track of a single emitter for unlimited periods of time. Furthermore, our design allows scanning the excitation spot and detection area independently of the sample position. This feature provides the means to perform probeless transport experiments on one-dimensional nanostructures. The scanning capabilities of this microscope are fully detailed and characterized using the photoluminescence of single nitrogen dyads at 4.5 K.

  4. Second harmonic super-resolution microscopy for quantification of mRNA at single copy sensitivity.

    PubMed

    Liu, Jing; Cho, Il-Hoon; Cui, Yi; Irudayaraj, Joseph

    2014-12-23

    Cell-specific information on the quantity and localization of key mRNAs at single copy sensitivity in single cells is critical for evaluating basic cellular process, disease risk, and efficacy of therapy. Quantification of overexpressed mRNAs beyond the diffraction limit is constrained by the optical property of the probes and microscopy techniques. In this report, nanosized barium titanium oxide (BaTiO3, BTO) crystals were utilized as probes for mRNA quantification by a second harmonic super-resolution microscopy (SHaSM). The SHaSM was able to detect a single copy of the human epidermal growth factor receptor 2 (Her2) mRNA at a resolution of 55.6 nm with the ability to resolve multiple mRNA copies in a diffraction-limited spot. Her2 mRNA per cell was counted in SK-BR-3, MCF-7, and HeLa cell lines as 595±79.1, 38.9±8.26, and 1.5±2.8, respectively. Our single-cell quantification results were validated with the fluorescence in situ hybridization studies and quantitative PCR, showing better specificity and selectivity over current single-molecule approaches for transcript detection. The SHaSM is expected to have an upper limit of resolving ∼10(4) transcripts in a single cell with the ability to monitor intracellular transcriptional dynamics at video rate. The developed approach has strong potential in clinical research and in the early diagnosis of life-threatening diseases such as cancer.

  5. High angular resolution at LBT

    NASA Astrophysics Data System (ADS)

    Conrad, A.; Arcidiacono, C.; Bertero, M.; Boccacci, P.; Davies, A. G.; Defrere, D.; de Kleer, K.; De Pater, I.; Hinz, P.; Hofmann, K. H.; La Camera, A.; Leisenring, J.; Kürster, M.; Rathbun, J. A.; Schertl, D.; Skemer, A.; Skrutskie, M.; Spencer, J. R.; Veillet, C.; Weigelt, G.; Woodward, C. E.

    2015-12-01

    High angular resolution from ground-based observatories stands as a key technology for advancing planetary science. In the window between the angular resolution achievable with 8-10 meter class telescopes, and the 23-to-40 meter giants of the future, LBT provides a glimpse of what the next generation of instruments providing higher angular resolution will provide. We present first ever resolved images of an Io eruption site taken from the ground, images of Io's Loki Patera taken with Fizeau imaging at the 22.8 meter LBT [Conrad, et al., AJ, 2015]. We will also present preliminary analysis of two data sets acquired during the 2015 opposition: L-band fringes at Kurdalagon and an occultation of Loki and Pele by Europa (see figure). The light curves from this occultation will yield an order of magnitude improvement in spatial resolution along the path of ingress and egress. We will conclude by providing an overview of the overall benefit of recent and future advances in angular resolution for planetary science.

  6. High resolution A/D conversion based on piecewise conversion at lower resolution

    DOEpatents

    Terwilliger, Steve [Albuquerque, NM

    2012-06-05

    Piecewise conversion of an analog input signal is performed utilizing a plurality of relatively lower bit resolution A/D conversions. The results of this piecewise conversion are interpreted to achieve a relatively higher bit resolution A/D conversion without sampling frequency penalty.

  7. Wavefront correction and high-resolution in vivo OCT imaging with an objective integrated multi-actuator adaptive lens.

    PubMed

    Bonora, Stefano; Jian, Yifan; Zhang, Pengfei; Zam, Azhar; Pugh, Edward N; Zawadzki, Robert J; Sarunic, Marinko V

    2015-08-24

    Adaptive optics is rapidly transforming microscopy and high-resolution ophthalmic imaging. The adaptive elements commonly used to control optical wavefronts are liquid crystal spatial light modulators and deformable mirrors. We introduce a novel Multi-actuator Adaptive Lens that can correct aberrations to high order, and which has the potential to increase the spread of adaptive optics to many new applications by simplifying its integration with existing systems. Our method combines an adaptive lens with an imaged-based optimization control that allows the correction of images to the diffraction limit, and provides a reduction of hardware complexity with respect to existing state-of-the-art adaptive optics systems. The Multi-actuator Adaptive Lens design that we present can correct wavefront aberrations up to the 4th order of the Zernike polynomial characterization. The performance of the Multi-actuator Adaptive Lens is demonstrated in a wide field microscope, using a Shack-Hartmann wavefront sensor for closed loop control. The Multi-actuator Adaptive Lens and image-based wavefront-sensorless control were also integrated into the objective of a Fourier Domain Optical Coherence Tomography system for in vivo imaging of mouse retinal structures. The experimental results demonstrate that the insertion of the Multi-actuator Objective Lens can generate arbitrary wavefronts to correct aberrations down to the diffraction limit, and can be easily integrated into optical systems to improve the quality of aberrated images.

  8. Wavefront correction and high-resolution in vivo OCT imaging with an objective integrated multi-actuator adaptive lens

    PubMed Central

    Bonora, Stefano; Jian, Yifan; Zhang, Pengfei; Zam, Azhar; Pugh, Edward N.; Zawadzki, Robert J.; Sarunic, Marinko V.

    2015-01-01

    Adaptive optics is rapidly transforming microscopy and high-resolution ophthalmic imaging. The adaptive elements commonly used to control optical wavefronts are liquid crystal spatial light modulators and deformable mirrors. We introduce a novel Multi-actuator Adaptive Lens that can correct aberrations to high order, and which has the potential to increase the spread of adaptive optics to many new applications by simplifying its integration with existing systems. Our method combines an adaptive lens with an imaged-based optimization control that allows the correction of images to the diffraction limit, and provides a reduction of hardware complexity with respect to existing state-of-the-art adaptive optics systems. The Multi-actuator Adaptive Lens design that we present can correct wavefront aberrations up to the 4th order of the Zernike polynomial characterization. The performance of the Multi-actuator Adaptive Lens is demonstrated in a wide field microscope, using a Shack-Hartmann wavefront sensor for closed loop control. The Multi-actuator Adaptive Lens and image-based wavefront-sensorless control were also integrated into the objective of a Fourier Domain Optical Coherence Tomography system for in vivo imaging of mouse retinal structures. The experimental results demonstrate that the insertion of the Multi-actuator Objective Lens can generate arbitrary wavefronts to correct aberrations down to the diffraction limit, and can be easily integrated into optical systems to improve the quality of aberrated images. PMID:26368169

  9. DVD pickup head based optical resolution photoacoustic microscopy

    NASA Astrophysics Data System (ADS)

    Wang, Po-Hsun; Li, Meng-Lin

    2012-02-01

    Optical resolution photoacoustic microscopy (OR-PAM) has been shown as a promising tool for label-free microvascular and single-cell imaging in clinical and bioscientific applications. However, most OR-PAM systems are realized by using a bulky laser for photoacoustic excitation. The large volume and high price of the laser may restrain the popularity of OR-PAM. In this study, we develop a low-cost and compact OR-PAM system based on a commercially available DVD pickup head. We showed that the DVD pickup head have the required laser energy and focusing optics for OR-PAM. The firmware of a DVD burner was modified to enable its laser diode to provide a 13-ns laser pulse with 1.3-nJ energy at 650 nm. Two excitation wavelengths at 650 and 780 nm were available. The laser beam was focused onto the target after passing through a 0.6-mm thick DVD transparent polycarbonate coating, and then aligned to be confocal with a 50-MHz focused ultrasonic transducer in forward mode. To keep the target on focus, a scan involving auto-tracking procedure was performed. The lateral resolution was verified via cross-sectional imaging of a 6-μm carbon fiber. The measured -6 dB width of the carbon fiber was 6.66 μm which was in agreement with optical diffraction limit. The proposed OR-PAM has potential as an economically viable and compact blood screening tool available outside of large laboratories due to its low cost and portability. Furthermore, a better spatial resolution could be provided by using a blue ray DVD pickup head.

  10. Achieving closure at Fernald

    SciTech Connect

    Bradburne, John; Patton, Tisha C.

    2001-02-25

    When Fluor Fernald took over the management of the Fernald Environmental Management Project in 1992, the estimated closure date of the site was more than 25 years into the future. Fluor Fernald, in conjunction with DOE-Fernald, introduced the Accelerated Cleanup Plan, which was designed to substantially shorten that schedule and save taxpayers more than $3 billion. The management of Fluor Fernald believes there are three fundamental concerns that must be addressed by any contractor hoping to achieve closure of a site within the DOE complex. They are relationship management, resource management and contract management. Relationship management refers to the interaction between the site and local residents, regulators, union leadership, the workforce at large, the media, and any other interested stakeholder groups. Resource management is of course related to the effective administration of the site knowledge base and the skills of the workforce, the attraction and retention of qualified a nd competent technical personnel, and the best recognition and use of appropriate new technologies. Perhaps most importantly, resource management must also include a plan for survival in a flat-funding environment. Lastly, creative and disciplined contract management will be essential to effecting the closure of any DOE site. Fluor Fernald, together with DOE-Fernald, is breaking new ground in the closure arena, and ''business as usual'' has become a thing of the past. How Fluor Fernald has managed its work at the site over the last eight years, and how it will manage the new site closure contract in the future, will be an integral part of achieving successful closure at Fernald.

  11. High resolution tomographic instrument development

    SciTech Connect

    Not Available

    1992-08-01

    Our recent work has concentrated on the development of high-resolution PET instrumentation reflecting in part the growing importance of PET in nuclear medicine imaging. We have developed a number of positron imaging instruments and have the distinction that every instrument has been placed in operation and has had an extensive history of application for basic research and clinical study. The present program is a logical continuation of these earlier successes. PCR-I, a single ring positron tomograph was the first demonstration of analog coding using BGO. It employed 4 mm detectors and is currently being used for a wide range of biological studies. These are of immense importance in guiding the direction for future instruments. In particular, PCR-II, a volume sensitive positron tomograph with 3 mm spatial resolution has benefited greatly from the studies using PCR-I. PCR-II is currently in the final stages of assembly and testing and will shortly be placed in operation for imaging phantoms, animals and ultimately humans. Perhaps the most important finding resulting from our previous study is that resolution and sensitivity must be carefully balanced to achieve a practical high resolution system. PCR-II has been designed to have the detection characteristics required to achieve 3 mm resolution in human brain under practical imaging situations. The development of algorithms by the group headed by Dr. Chesler is based on a long history of prior study including his joint work with Drs. Pelc and Reiderer and Stearns. This body of expertise will be applied to the processing of data from PCR-II when it becomes operational.

  12. High resolution tomographic instrument development

    SciTech Connect

    Not Available

    1992-01-01

    Our recent work has concentrated on the development of high-resolution PET instrumentation reflecting in part the growing importance of PET in nuclear medicine imaging. We have developed a number of positron imaging instruments and have the distinction that every instrument has been placed in operation and has had an extensive history of application for basic research and clinical study. The present program is a logical continuation of these earlier successes. PCR-I, a single ring positron tomograph was the first demonstration of analog coding using BGO. It employed 4 mm detectors and is currently being used for a wide range of biological studies. These are of immense importance in guiding the direction for future instruments. In particular, PCR-II, a volume sensitive positron tomograph with 3 mm spatial resolution has benefited greatly from the studies using PCR-I. PCR-II is currently in the final stages of assembly and testing and will shortly be placed in operation for imaging phantoms, animals and ultimately humans. Perhaps the most important finding resulting from our previous study is that resolution and sensitivity must be carefully balanced to achieve a practical high resolution system. PCR-II has been designed to have the detection characteristics required to achieve 3 mm resolution in human brain under practical imaging situations. The development of algorithms by the group headed by Dr. Chesler is based on a long history of prior study including his joint work with Drs. Pelc and Reiderer and Stearns. This body of expertise will be applied to the processing of data from PCR-II when it becomes operational.

  13. High resolution tomographic instrument development

    NASA Astrophysics Data System (ADS)

    Our recent work has concentrated on the development of high-resolution PET instrumentation reflecting in part the growing importance of PET in nuclear medicine imaging. We have developed a number of positron imaging instruments and have the distinction that every instrument has been placed in operation and has had an extensive history of application for basic research and clinical study. The present program is a logical continuation of these earlier successes. PCR-I, a single ring positron tomograph was the first demonstration of analog coding using BGO. It employed 4 mm detectors and is currently being used for a wide range of biological studies. These are of immense importance in guiding the direction for future instruments. In particular, PCR-II, a volume sensitive positron tomograph with 3 mm spatial resolution has benefitted greatly from the studies using PCR-I. PCR-II is currently in the final stages of assembly and testing and will shortly be placed in operation for imaging phantoms, animals and ultimately humans. Perhaps the most important finding resulting from our previous study is that resolution and sensitivity must be carefully balanced to achieve a practical high resolution system. PCR-II has been designed to have the detection characteristics required to achieve 3 mm resolution in human brain under practical imaging situations. The development of algorithms by the group headed by Dr. Chesler is based on a long history of prior study including his joint work with Drs. Pelc and Reiderer and Stearns. This body of expertise will be applied to the processing of data from PCR-II when it becomes operational.

  14. Resolution limits for imaging through multi-mode fiber.

    PubMed

    Mahalati, Reza Nasiri; Gu, Ruo Yu; Kahn, Joseph M

    2013-01-28

    We experimentally demonstrate endoscopic imaging through a multi-mode fiber (MMF) in which the number of resolvable image features approaches four times the number of spatial modes per polarization propagating in the fiber. In our method, a sequence of random field patterns is input to the fiber, generating a sequence of random intensity patterns at the output, which are used to sample an object. Reflected power values are returned through the fiber and linear optimization is used to reconstruct an image. The factor-of-four resolution enhancement is due to mixing of modes by the squaring inherent in field-to-intensity conversion. The incoherent point-spread function (PSF) at the center of the fiber output plane is an Airy disk equivalent to the coherent PSF of a conventional diffraction-limited imaging system having a numerical aperture twice that of the fiber. All previous methods for imaging through MMF can only resolve a number of features equal to the number of modes. Most of these methods use localized intensity patterns for sampling the object and use local image reconstruction.

  15. Structure information preserved optical remote sensing images super-resolution reconstruction with prior model

    NASA Astrophysics Data System (ADS)

    Sun, Tao; Lin, Liyu; Zhang, Feiyan; Tu, Yafei; Qin, Qianqing

    2009-10-01

    The satellite imaging system is affected by optical diffraction limitation, atmosphere disturbance, CCD under-sampling noise and etc. Then the information beyond image system cut-off frequency is lost, images degrade and spatial resolution decrease. Structure information becomes undistinguishable, which is fatal to manual interpretation and adaptive target recognition. In this paper, one structure information preserved scheme is proposed. Taking into account the anisotropic diffuse property of PSF (point spread function) of in-track and cross-track direction, the sparse property of nature image and noise level, with data-driven kernel function sub-pixel estimation, the method restore high spatial resolution image from low one. Joint frequency domain and wavelet domain L1 normal regularization suppress wrinkle and noise amplified for this ill-posed inverse problem. With CBERS-2 images, this method is proved to improve spatial resolution and preserve edge and structure effectively without obverse wrinkle. With MTF curve, the spatial resolution is improved obviously with high PSNR, and the edge is preserved perfectly.

  16. Imaging live cells at high spatiotemporal resolution for lab-on-a-chip applications.

    PubMed

    Chin, Lip Ket; Lee, Chau-Hwang; Chen, Bi-Chang

    2016-05-24

    Conventional optical imaging techniques are limited by the diffraction limit and difficult-to-image biomolecular and sub-cellular processes in living specimens. Novel optical imaging techniques are constantly evolving with the desire to innovate an imaging tool that is capable of seeing sub-cellular processes in a biological system, especially in three dimensions (3D) over time, i.e. 4D imaging. For fluorescence imaging on live cells, the trade-offs among imaging depth, spatial resolution, temporal resolution and photo-damage are constrained based on the limited photons of the emitters. The fundamental solution to solve this dilemma is to enlarge the photon bank such as the development of photostable and bright fluorophores, leading to the innovation in optical imaging techniques such as super-resolution microscopy and light sheet microscopy. With the synergy of microfluidic technology that is capable of manipulating biological cells and controlling their microenvironments to mimic in vivo physiological environments, studies of sub-cellular processes in various biological systems can be simplified and investigated systematically. In this review, we provide an overview of current state-of-the-art super-resolution and 3D live cell imaging techniques and their lab-on-a-chip applications, and finally discuss future research trends in new and breakthrough research areas of live specimen 4D imaging in controlled 3D microenvironments.

  17. Intensity and phase fields behind Phase Shifting Masks studied with High Resolution Interference Microscopy

    NASA Astrophysics Data System (ADS)

    Puthankovilakam, Krishnaparvathy; Scharf, Toralf; Herzig, Hans Peter; Weichelt, Tina; Zeitner, Uwe; Vogler, Uwe; Voelkel, Reinhard

    2015-03-01

    The proximity printing industry is in real need of high resolution results and it can be done using Phase Shift Mask (PSM) or by applying Optical Proximity Correction (OPC). In our research we are trying to find out details of how light fields behind the structures of photo masks develop in order to determine the best conditions and designs for proximity printing. We focus here on parameters that are used in real situation with gaps up to 50 μm and structure sizes down to 2 μm. The light field evolution behind the structures is studied and delivers insight in to precisions and tolerances that need to be respected. It is the first time that an experimental analysis of light propagation through mask is presented in detail, which includes information on intensity and phase. The instrument we use is known as High Resolution Interference Microscopy (HRIM). HRIM is a Mach-Zehnder interferometer which is capable of recording three dimensional distributions of intensity and phase with diffraction limited resolution. Our characterization technique allows plotting the evolution of the desired light field and therefore printable structure till the desired proximity gap. In this paper we discuss in detail the evolution of intensity and phase fields of elbow or corner structure at different position behind a phase mask and interpret the main parameters. Of particular interest are tolerances against proximity gap variation and the resolution in printed structures.

  18. Wide-field multispectral super-resolution imaging using spin-dependent fluorescence in nanodiamonds.

    PubMed

    Chen, Edward H; Gaathon, Ophir; Trusheim, Matthew E; Englund, Dirk

    2013-05-08

    Recent advances in fluorescence microscopy have enabled spatial resolution below the diffraction limit by localizing multiple temporally or spectrally distinguishable fluorophores. Here, we introduce a super-resolution technique that deterministically controls the brightness of uniquely addressable, photostable emitters. We modulate the fluorescence brightness of negatively charged nitrogen-vacancy (NV(-)) centers in nanodiamonds through magnetic resonance techniques. Using a CCD camera, this "deterministic emitter switch microscopy" (DESM) technique enables super-resolution imaging with localization down to 12 nm across a 35 × 35 μm(2) area. DESM is particularly well suited for biological applications such as multispectral particle tracking since fluorescent nanodiamonds are not only cytocompatible but also nonbleaching and bright. We observe fluorescence count rates exceeding 1.5 × 10(6) photons per second from single NV(-) centers at saturation. When combined with emerging NV(-)-based techniques for sensing magnetic and electric fields, DESM opens the door to rapid, super-resolution imaging for tracking and sensing applications in the life and physical sciences.

  19. Fluorescence in situ hybridization applications for super-resolution 3D structured illumination microscopy.

    PubMed

    Markaki, Yolanda; Smeets, Daniel; Cremer, Marion; Schermelleh, Lothar

    2013-01-01

    Fluorescence in situ hybridization on three-dimensionally preserved cells (3D-FISH) is an efficient tool to analyze the subcellular localization and spatial arrangement of targeted DNA sequences and RNA transcripts at the single cell level. 3D reconstructions from serial optical sections obtained by confocal laser scanning microscopy (CLSM) have long been considered the gold standard for 3D-FISH analyses. Recent super-resolution techniques circumvent the diffraction-limit of optical resolution and have defined a new state-of-the-art in bioimaging. Three-dimensional structured illumination microscopy (3D-SIM) represents one of these technologies. Notably, 3D-SIM renders an eightfold improved volumetric resolution over conventional imaging, and allows the simultaneous visualization of differently labeled target structures. These features make this approach highly attractive for the analysis of spatial relations and substructures of nuclear targets that escape detection by conventional light microscopy. Here, we focus on the application of 3D-SIM for the visualization of subnuclear 3D-FISH preparations. In comparison with conventional fluorescence microscopy, the quality of 3D-SIM data is dependent to a much greater extent on the optimal sample preparation, labeling and acquisition conditions. We describe typical problems encountered with super-resolution imaging of in situ hybridizations in mammalian tissue culture cells and provide optimized DNA-/(RNA)-FISH protocols including combinations with immunofluorescence staining (Immuno-FISH) and DNA replication labeling using click chemistry.

  20. Further resolution enhancement of high-sensitivity laser scanning photothermal microscopy applied to mouse endogenous

    NASA Astrophysics Data System (ADS)

    Nakata, Kazuaki; Tsurui, Hiromichi; Kobayashi, Takayoshi

    2016-12-01

    Photothermal microscopy has intrinsically super resolution capability due to the bilinear dependence of signal intensity of pump and probe. In the present paper, we have made further resolution improvement of high-sensitivity laser scanning photothermal microscopy by applying non-linear detection. By this, the new method has the following advantages: (1) super resolution with 61% and 42% enhancement from the diffraction limit values of the probe and pump wavelengths, respectively, by a second-order non-linear scheme, (2) compact light source using inexpensive conventional diode lasers, (3) wide applicability to nonfluorescent materials such as gold nanoparticles (GNPs) and hematoxylin-eosin stained biological samples, (4) relative robustness to optical damage, and (5) a high-frame rate using a Galvano mirror. The maximum resolution is determined to be 160 nm in the second-order non-linear detection mode and 270 nm in the linear detection mode by the PT signal of GNPs. The pixel rate and frame rate for 300 × 300 pixel image are 50 μs and 4.5 s, respectively. The pixel and frame rate are shorter than the rates, which are 1 ms and 100 s, respectively, using the piezo-driven stage system.

  1. High-efficiency and high-resolution apertureless plasmonic near-field probe under internal illumination

    NASA Astrophysics Data System (ADS)

    Jiang, R. H.; Chou, H. C.; Chu, J. Y.; Chen, C.; Yen, T. J.

    2016-09-01

    Near-field scanning optical microscopy (NSOM) offers subwavelength optical resolution beyond the diffraction limit, enabling practical applications in optical imaging, sensing and nanolithography. However, due to the sub-100 nm size of apertures, conventional NSOM aperture probes suffer from the constrains of the strong attenuation of the throughput and limited the spatial resolution. To solve the problem, we designed a novel scheme for apertureless plasmonic probes with radial internal illumination. Employing non-periodic multi-rings geometry for plasmonic excitations, surface plasmons adiabatically nanofocuse energy at tip and the full width at half maximum of the optimal design is 18 nm. The proposed probe was optimized with 2D finite-difference time-domain (FDTD) analysis and realistic parabolic probe geometries. Comprehensive electromagnetic simulation shows that the optimal probe feature obeys Fabry-Pérot condition on the plasmonic metallic wall, giving rise to substantial field enhancement up to 6 orders of magnitude greater than conventional aperture probes without degrading its spatial resolution. We fabricated the proposed probe which possesses apex angle ( 22 degree) and tip radius ( 30 nm). Finally, the proposed near field plasmonic probe effectively combining the high resolution of apertureless probes with high throughput can enable the proposed plasmonic NSOM probe as a practical tool for applications in near field optical microscopy.

  2. Achievement Goals and Achievement Emotions: A Meta-Analysis

    ERIC Educational Resources Information Center

    Huang, Chiungjung

    2011-01-01

    This meta-analysis synthesized 93 independent samples (N = 30,003) in 77 studies that reported in 78 articles examining correlations between achievement goals and achievement emotions. Achievement goals were meaningfully associated with different achievement emotions. The correlations of mastery and mastery approach goals with positive achievement…

  3. Case Resolution Manual

    EPA Pesticide Factsheets

    This Case Resolution Manual (CRM) is intended to provide procedural guidance to ECRCO case managers to ensure EPA’s prompt, effective, and efficient resolution of civil rights cases consistent with science and the civil rights laws.

  4. Resolution with Limited Factoring

    NASA Astrophysics Data System (ADS)

    Li, Dafa

    The resolution principle was originally proposed by J.A. Robinson. Resolution with factoring rule is complete for the first-order logic. However, unlimited applications of factoring rule may generate many irrelevant and redundant clauses. Noll presented resolution rule with half-factoring. In this paper, we demonstrate how to eliminate the half-factoring.

  5. Coherence versus interferometric resolution

    SciTech Connect

    Luis, Alfredo

    2010-06-15

    We examine the relation between second-order coherence and resolution in the interferometric detection of phase shifts. While for classical thermal light resolution and second-order coherence are synonymous, we show that for quantum light beams reaching optimum precision second-order coherence and resolution become antithetical.

  6. Atomic resolution holography.

    PubMed

    Hayashi, Kouichi

    2014-11-01

    Atomic resolution holography, such as X-ray fluorescence holography (XFH)[1] and photoelectron holography (PH), has the attention of researcher as an informative local structure analysis, because it provides three dimensional atomic images around specific elements within a range of a few nanometers. It can determine atomic arrangements around a specific element without any prior knowledge of structures. It is considered that the atomic resolution holographic is a third method of structural analysis at the atomic level after X-ray diffraction (XRD) and X-ray absorption fine structure (XAFS). As known by many researchers, XRD and XAFS are established methods that are widespread use in various fields. XRD and XAFS provide information on long-range translational periodicities and very local environments, respectively, whereas the atomic resolution holography gives 3D information on the local order and can visualize surrounding atoms with a large range of coordination shells. We call this feature "3D medium-range local structure observation".In addition to this feature, the atomic resolution holography is very sensitive to the displacement of atoms from their ideal positions, and one can obtain quantitative information about local lattice distortions by analyzing reconstructed atomic images[2] When dopants with different atomic radii from the matrix elements are present, the lattices around the dopants are distorted. However, using the conventional methods of structural analysis, one cannot determine the extent to which the local lattice distortions are preserved from the dopants. XFH is a good tool for solving this problem.Figure 1 shows a recent achievement on a relaxor ferroelectric of Pb(Mg1/3Nb2/3)O3 (PMN) using XFH. The structural studies of relaxor ferroelectrics have been carried out by X-ray or neutron diffractions, which suggested rhombohedral distortions of their lattices. However, their true pictures have not been obtained, yet. The Nb Kα holograms showed

  7. Tunable resolution terahertz dual frequency comb spectrometer.

    PubMed

    Vieira, Francisco S; Cruz, Flavio C; Plusquellic, David F; Diddams, Scott A

    2016-12-26

    Terahertz dual frequency comb spectroscopy (THz-DFCS) yields high spectral resolution without compromising bandwidth. Nonetheless, the resolution of THz-DFCS is usually limited by the laser repetition rate, which is typically between 80 MHz and 1 GHz. In this paper, we demonstrate a new method to achieve sub-repetition rate resolution in THz-DFCS by adaptively modifying the effective laser repetition rate using integrated Mach-Zehnder electro-optic modulators (MZ-EOMs). Our results demonstrate that it is possible to improve the 100 MHz resolution of a terahertz frequency comb by at least 20x (down to 5 MHz) across the terahertz spectrum without compromising the average output power, and to a large extent, its bandwidth. Our approach can augment a wide range of existing THz-DFCS systems to provide a significant and easily adaptable resolution improvement.

  8. Energy resolution enhancement of mercuric iodide detectors

    NASA Technical Reports Server (NTRS)

    Finger, M.; Prince, T. A.; Padgett, L.; Prickett, B.; Schnepple, W.

    1984-01-01

    A pulse processing technique has been developed which improves the gamma-ray energy resolution of mercuric iodide detectors. The technique employs a fast (100 ns) and a slow (6.4 microsec) pulse height analysis to correct for signal variations due to variations in charge trapping. The capabilities of the technique for energy resolution enhancement are discussed as well as the utility of the technique for examining the trapping characteristics of individual detectors. An energy resolution of 2.6 percent FWHM at 662 keV was achieved with an acceptance efficiency of 100 percent from a mercuric iodide detector which gives 8.3 percent FWHM using standard techniques.

  9. A resolution expressing the gratitude and appreciation of the Senate for the acts of heroism and military achievement by the members of the United States Armed Forces who participated in the June 6, 1944, amphibious landing at Normandy, France, and commending them for leadership and valor in an operation that helped bring an end to World War II.

    THOMAS, 113th Congress

    Sen. Boozman, John [R-AR

    2014-04-10

    05/21/2014 Resolution agreed to in Senate without amendment and with a preamble by Unanimous Consent. (consideration: CR S3243) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  10. Tip-tilt compensation: Resolution limits for ground-based telescopes using laser guide star adaptive optics. Revision 2

    SciTech Connect

    Olivier, S.S.; Max, C.E.; Gavel, D.T.; Brase, J.M.

    1992-10-08

    The angular resolution of long-exposure images from ground-based telescopes equipped with laser guide star adaptive optics systems is fundamentally limited by the the accuracy with which the tip-tilt aberrations introduced by the atmosphere can be corrected. Assuming that a natural star is used as the tilt reference, the residual error due to tilt anisoplanatism can significantly degrade the long-exposure resolution even if the tilt reference star is separated from the object being imaged by a small angle. Given the observed distribution of stars in the sky, the need to find a tilt reference star quite close to the object restricts the fraction of the sky over which long-exposure images with diffraction limited resolution can be obtained. In this paper, the authors present a comprehensive performance analysis of tip-tilt compensation systems that use a natural star as a tilt reference, taking into account properties of the atmosphere and of the Galactic stellar populations, and optimizing over the system operating parameters to determine the fundamental limits to the long-exposure resolution. Their results show that for a ten meter telescope on Mauna Kea, if the image of the tilt reference star is uncorrected, about half the sky can be imaged in the V band with long-exposure resolution less than 60 milli-arc-seconds (mas), while if the image of the tilt reference star is fully corrected, about half the sky can be imaged in the V band with long-exposure resolution less than 16 mas. Furthermore, V band images long-exposure resolution of less than 16 mas may be obtained with a ten meter telescope on Mauna Kea for unresolved objects brighter than magnitude 22 that are fully corrected by a laser guide star adaptive optics system. This level of resolution represents about 70% of the diffraction limit of a ten meter telescope in the V band and is more than a factor of 45 better than the median seeing in the V band on Mauna Kea.

  11. Lifting Minority Achievement: Complex Answers. The Achievement Gap.

    ERIC Educational Resources Information Center

    Viadero, Debra; Johnston, Robert C.

    2000-01-01

    This fourth in a four-part series on why academic achievement gaps exist describes the Minority Achievement Committee scholars program at Shaker Heights High School in Cleveland, Ohio, a powerful antidote to the achievement gap between minority and white and Asian American students. It explains the need to break down stereotypes about academic…

  12. Achievement Motivation of Women: Effects of Achievement and Affiliation Arousal.

    ERIC Educational Resources Information Center

    Gama, Elizabeth Maria Pinheiro

    1985-01-01

    Assigned 139 Brazilian women to neutral, affiliation arousal, and achievement arousal conditions based on their levels of achievement (Ach) and affiliative (Aff) needs. Results of story analyses revealed that achievement arousal increased scores of high Ach subjects and that high Aff subjects obtained higher scores than low Aff subjects. (BL)

  13. Attitude Towards Physics and Additional Mathematics Achievement Towards Physics Achievement

    ERIC Educational Resources Information Center

    Veloo, Arsaythamby; Nor, Rahimah; Khalid, Rozalina

    2015-01-01

    The purpose of this research is to identify the difference in students' attitude towards Physics and Additional Mathematics achievement based on gender and relationship between attitudinal variables towards Physics and Additional Mathematics achievement with achievement in Physics. This research focused on six variables, which is attitude towards…

  14. The Impact of Reading Achievement on Overall Academic Achievement

    ERIC Educational Resources Information Center

    Churchwell, Dawn Earheart

    2009-01-01

    This study examined the relationship between reading achievement and achievement in other subject areas. The purpose of this study was to determine if there was a correlation between reading scores as measured by the Standardized Test for the Assessment of Reading (STAR) and academic achievement in language arts, math, science, and social studies…

  15. Establishing super-resolution imaging for proteins in diatom biosilica

    PubMed Central

    Gröger, Philip; Poulsen, Nicole; Klemm, Jennifer; Kröger, Nils; Schlierf, Michael

    2016-01-01

    The intricate, genetically controlled biosilica nano- and micropatterns produced by diatoms are a testimony for biology’s ability to control mineral formation (biomineralization) at the nanoscale and regarded as paradigm for nanotechnology. Previously, several protein families involved in diatom biosilica formation have been identified, and many of them remain tightly associated with the final biosilica structure. Determining the locations of biosilica-associated proteins with high precision is, therefore expected to provide clues to their roles in biosilica morphogenesis. To achieve this, we introduce here single-molecule localization microscopy to diatoms based on photo-activated light microscopy (PALM) to overcome the diffraction limit. We identified six photo-convertible fluorescent proteins (FPs) that can be utilized for PALM in the cytoplasm of model diatom Thalassiosira pseudonana. However, only three FPs were also functional when embedded in diatom biosilica. These were employed for PALM-based localization of the diatom biosilica-associated protein Silaffin-3 (tpSil3) with a mean precision of 25 nm. This allowed for the identification of distinct accumulation areas of Sil3 in the biosilica, which cannot be resolved by confocal fluorescence microscopy. The enhanced microscopy technique introduced here for diatoms will aid in elucidating the molecular mechanism of silica biomineralization as well as other aspects of diatom cell biology. PMID:27827427

  16. Interstellar scattering and resolution limitations

    NASA Astrophysics Data System (ADS)

    Dennison, Brian

    Density irregularities in both the interplanetary medium and the ionized component of the interstellar medium scatter radio waves, resulting in limitations on the achievable resolution. Interplanetary scattering (IPS) is weak for most observational situations, and in principle the resulting phase corruption can be corrected for when observing with sufficiently many array elements. Interstellar scattering (ISS), on the other hand, is usually strong at frequencies below about 8 GHz, in which case intrinsic structure information over a range of angular scales is irretrievably lost. With the earth-space baselines now planned, it will be possible to search directly for interstellar refraction, which is suspected of modulating the fluxes of background sources.

  17. A High Resolution Ammunition Resupply Model.

    DTIC Science & Technology

    1982-03-01

    Transportation Assets .. . . . . . . . 111 b. Maximization of Shipping Space . . . . . 112 c. Adjustments Due to Priority Requisitions. 112 3. RESUPPLY...planned logistics module was expanded to a full stand-alone, high resolution model. Supplementary objectives were established in order to achieve the...each variable, and replication of the process described by these variables in order to achieve an expected value outcome. Using this technique, the

  18. Actin restructuring during Salmonella typhimurium infection investigated by confocal and super-resolution microscopy

    NASA Astrophysics Data System (ADS)

    Han, Jason J.; Kunde, Yuliya A.; Hong-Geller, Elizabeth; Werner, James H.

    2014-01-01

    We have used super-resolution optical microscopy and confocal microscopy to visualize the cytoskeletal restructuring of HeLa cells that accompanies and enables Salmonella typhimurium internalization. Herein, we report the use of confocal microscopy to verify and explore infection conditions that would be compatible with super-resolution optical microscopy, using Alexa-488 labeled phalloidin to stain the actin cytoskeletal network. While it is well known that actin restructuring and cytoskeletal rearrangements often accompany and assist in bacterial infection, most studies have employed conventional diffraction-limited fluorescence microscopy to explore these changes. Here we show that the superior spatial resolution provided by single-molecule localization methods (such as direct stochastic optical reconstruction microscopy) enables more precise visualization of the nanoscale changes in the actin cytoskeleton that accompany bacterial infection. In particular, we found that a thin (100-nm) ring of actin often surrounds an invading bacteria 10 to 20 min postinfection, with this ring being transitory in nature. We estimate that a few hundred monofilaments of actin surround the S. typhimurium in this heretofore unreported bacterial internalization intermediate.

  19. HIGH RESOLUTION 36 GHz IMAGING OF THE SUPERNOVA REMNANT OF SN 1987A

    SciTech Connect

    Potter, T. M.; Staveley-Smith, L.; Zanardo, G.; Ng, C.-Y.; Gaensler, B. M.; Ball, Lewis; Kesteven, M. J.; Manchester, R. N.; Tzioumis, A. K.

    2009-11-01

    The aftermath of supernova (SN) 1987A continues to provide spectacular insights into the interaction between an SN blastwave and its circumstellar environment. We here present 36 GHz observations from the Australia Telescope Compact Array of the radio remnant of SN 1987A. These new images, taken in 2008 April and 2008 October, substantially extend the frequency range of an ongoing monitoring and imaging program conducted between 1.4 and 20 GHz. Our 36.2 GHz images have a diffraction-limited angular resolution of 0.''3-0.''4, which covers the gap between high resolution, low dynamic range VLBI images of the remnant and low resolution, high dynamic range images at frequencies between 1 and 20 GHz. The radio morphology of the remnant at 36 GHz is an elliptical ring with enhanced emission on the eastern and western sides, similar to that seen previously at lower frequencies. Model fits to the data in the Fourier domain show that the emitting region is consistent with a thick inclined torus of mean radius 0.''85, and a 2008 October flux density of 27 +- 6 mJy at 36.2 GHz. The spectral index for the remnant at this epoch, determined between 1.4 GHz and 36.2 GHz, is alpha = -0.83. There is tentative evidence for an unresolved central source with flatter spectral index.

  20. Super Resolution Imaging of Genetically Labeled Synapses in Drosophila Brain Tissue

    PubMed Central

    Spühler, Isabelle A.; Conley, Gaurasundar M.; Scheffold, Frank; Sprecher, Simon G.

    2016-01-01

    Understanding synaptic connectivity and plasticity within brain circuits and their relationship to learning and behavior is a fundamental quest in neuroscience. Visualizing the fine details of synapses using optical microscopy remains however a major technical challenge. Super resolution microscopy opens the possibility to reveal molecular features of synapses beyond the diffraction limit. With direct stochastic optical reconstruction microscopy, dSTORM, we image synaptic proteins in the brain tissue of the fruit fly, Drosophila melanogaster. Super resolution imaging of brain tissue harbors difficulties due to light scattering and the density of signals. In order to reduce out of focus signal, we take advantage of the genetic tools available in the Drosophila and have fluorescently tagged synaptic proteins expressed in only a small number of neurons. These neurons form synapses within the calyx of the mushroom body, a distinct brain region involved in associative memory formation. Our results show that super resolution microscopy, in combination with genetically labeled synaptic proteins, is a powerful tool to investigate synapses in a quantitative fashion providing an entry point for studies on synaptic plasticity during learning and memory formation. PMID:27303270

  1. A Compact "Water Window" Microscope with 60 nm Spatial Resolution for Applications in Biology and Nanotechnology.

    PubMed

    Wachulak, Przemyslaw; Torrisi, Alfio; Nawaz, Muhammad F; Bartnik, Andrzej; Adjei, Daniel; Vondrová, Šárka; Turňová, Jana; Jančarek, Alexandr; Limpouch, Jiří; Vrbová, Miroslava; Fiedorowicz, Henryk

    2015-10-01

    Short illumination wavelength allows an extension of the diffraction limit toward nanometer scale; thus, improving spatial resolution in optical systems. Soft X-ray (SXR) radiation, from "water window" spectral range, λ=2.3-4.4 nm wavelength, which is particularly suitable for biological imaging due to natural optical contrast provides better spatial resolution than one obtained with visible light microscopes. The high contrast in the "water window" is obtained because of selective radiation absorption by carbon and water, which are constituents of the biological samples. The development of SXR microscopes permits the visualization of features on the nanometer scale, but often with a tradeoff, which can be seen between the exposure time and the size and complexity of the microscopes. Thus, herein, we present a desk-top system, which overcomes the already mentioned limitations and is capable of resolving 60 nm features with very short exposure time. Even though the system is in its initial stage of development, we present different applications of the system for biology and nanotechnology. Construction of the microscope with recently acquired images of various samples will be presented and discussed. Such a high resolution imaging system represents an interesting solution for biomedical, material science, and nanotechnology applications.

  2. Actin restructuring during Salmonella typhimurium infection investigated by confocal and super-resolution microscopy.

    PubMed

    Han, Jason J; Kunde, Yuliya A; Hong-Geller, Elizabeth; Werner, James H

    2014-01-01

    We have used super-resolution optical microscopy and confocal microscopy to visualize the cytoskeletal restructuring of HeLa cells that accompanies and enables Salmonella typhimurium internalization. Herein, we report the use of confocal microscopy to verify and explore infection conditions that would be compatible with super-resolution optical microscopy, using Alexa-488 labeled phalloidin to stain the actin cytoskeletal network. While it is well known that actin restructuring and cytoskeletal rearrangements often accompany and assist in bacterial infection, most studies have employed conventional diffraction-limited fluorescence microscopy to explore these changes. Here we show that the superior spatial resolution provided by single-molecule localization methods (such as direct stochastic optical reconstruction microscopy) enables more precise visualization of the nanoscale changes in the actin cytoskeleton that accompany bacterial infection. In particular, we found that a thin (100-nm) ring of actin often surrounds an invading bacteria 10 to 20 min postinfection, with this ring being transitory in nature. We estimate that a few hundred monofilaments of actin surround the S. typhimurium in this heretofore unreported bacterial internalization intermediate.

  3. Resolving the spatial relationship between intracellular components by dual color super resolution optical fluctuations imaging (SOFI)

    PubMed Central

    Gallina, Maria Elena; Xu, Jianmin; Dertinger, Thomas; Aizer, Adva; Shav-Tal, Yaron; Weiss, Shimon

    2013-01-01

    Background Multi-color super-resolution (SR) imaging microscopy techniques can resolve ultrastructura relationships between- and provide co-localization information of- different proteins inside the cell or even within organelles at a higher resolution than afforded by conventional diffraction-limited imaging. While still very challenging, important SR colocalization results have been reported in recent years using STED, PALM and STORM techniques. Results In this work, we demonstrate dual-color Super Resolution Optical Fluctuations Imaging (SOFI) using a standard far-field fluorescence microscope and different color blinking quantum dots. We define the spatial relationship between hDcp1a, a processing body (P-body, PB) protein, and the tubulin cytoskeletal network. Our finding could open up new perspectives on the role of the cytoskeleton in PB formation and assembly. Further insights into PB internal organization are also reported and discussed. Conclusions Our results demonstrate the suitability and facile use of multi-color SOFI for the investigation of intracellular ultrastructures. PMID:24324919

  4. A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging

    PubMed Central

    Sofroniew, Nicholas James; Flickinger, Daniel; King, Jonathan; Svoboda, Karel

    2016-01-01

    Imaging is used to map activity across populations of neurons. Microscopes with cellular resolution have small (<1 millimeter) fields of view and cannot simultaneously image activity distributed across multiple brain areas. Typical large field of view microscopes do not resolve single cells, especially in the axial dimension. We developed a 2-photon random access mesoscope (2p-RAM) that allows high-resolution imaging anywhere within a volume spanning multiple brain areas (∅ 5 mm x 1 mm cylinder). 2p-RAM resolution is near diffraction limited (lateral, 0.66 μm, axial 4.09 μm at the center; excitation wavelength = 970 nm; numerical aperture = 0.6) over a large range of excitation wavelengths. A fast three-dimensional scanning system allows efficient sampling of neural activity in arbitrary regions of interest across the entire imaging volume. We illustrate the use of the 2p-RAM by imaging neural activity in multiple, non-contiguous brain areas in transgenic mice expressing protein calcium sensors. DOI: http://dx.doi.org/10.7554/eLife.14472.001 PMID:27300105

  5. FPscope: a field-portable high-resolution microscope using a cellphone lens

    PubMed Central

    Dong, Siyuan; Guo, Kaikai; Nanda, Pariksheet; Shiradkar, Radhika; Zheng, Guoan

    2014-01-01

    The large consumer market has made cellphone lens modules available at low-cost and in high-quality. In a conventional cellphone camera, the lens module is used to demagnify the scene onto the image plane of the camera, where image sensor is located. In this work, we report a 3D-printed high-resolution Fourier ptychographic microscope, termed FPscope, which uses a cellphone lens in a reverse manner. In our platform, we replace the image sensor with sample specimens, and use the cellphone lens to project the magnified image to the detector. To supersede the diffraction limit of the lens module, we use an LED array to illuminate the sample from different incident angles and synthesize the acquired images using the Fourier ptychographic algorithm. As a demonstration, we use the reported platform to acquire high-resolution images of resolution target and biological specimens, with a maximum synthetic numerical aperture (NA) of 0.5. We also show that, the depth-of-focus of the reported platform is about 0.1 mm, orders of magnitude longer than that of a conventional microscope objective with a similar NA. The reported platform may enable healthcare accesses in low-resource settings. It can also be used to demonstrate the concept of computational optics for educational purposes. PMID:25360351

  6. Super-Resolution Imaging of a Dielectric Microsphere Is Governed by the Waist of Its Photonic Nanojet.

    PubMed

    Yang, Hui; Trouillon, Raphaël; Huszka, Gergely; Gijs, Martin A M

    2016-08-10

    Dielectric microspheres with appropriate refractive index can image objects with super-resolution, that is, with a precision well beyond the classical diffraction limit. A microsphere is also known to generate upon illumination a photonic nanojet, which is a scattered beam of light with a high-intensity main lobe and very narrow waist. Here, we report a systematic study of the imaging of water-immersed nanostructures by barium titanate glass microspheres of different size. A numerical study of the light propagation through a microsphere points out the light focusing capability of microspheres of different size and the waist of their photonic nanojet. The former correlates to the magnification factor of the virtual images obtained from linear test nanostructures, the biggest magnification being obtained with microspheres of ∼6-7 μm in size. Analyzing the light intensity distribution of microscopy images allows determining analytically the point spread function of the optical system and thereby quantifies its resolution. We find that the super-resolution imaging of a microsphere is dependent on the waist of its photonic nanojet, the best resolution being obtained with a 6 μm Ø microsphere, which generates the nanojet with the minimum waist. This comparison allows elucidating the super-resolution imaging mechanism.

  7. Achievements in Stratospheric Ozone Protection

    EPA Pesticide Factsheets

    This report describes achievements in protecting the ozone layer, the benefits of these achievements, and strategies involved (e.g., using alternatives to ozone-depleting substances, phasing out harmful substances, and creating partnerships).

  8. Students’ Achievement Goals, Learning-Related Emotions and Academic Achievement

    PubMed Central

    Lüftenegger, Marko; Klug, Julia; Harrer, Katharina; Langer, Marie; Spiel, Christiane; Schober, Barbara

    2016-01-01

    In the present research, the recently proposed 3 × 2 model of achievement goals is tested and associations with achievement emotions and their joint influence on academic achievement are investigated. The study was conducted with 388 students using the 3 × 2 Achievement Goal Questionnaire including the six proposed goal constructs (task-approach, task-avoidance, self-approach, self-avoidance, other-approach, other-avoidance) and the enjoyment and boredom scales from the Achievement Emotion Questionnaire. Exam grades were used as an indicator of academic achievement. Findings from CFAs provided strong support for the proposed structure of the 3 × 2 achievement goal model. Self-based goals, other-based goals and task-approach goals predicted enjoyment. Task-approach goals negatively predicted boredom. Task-approach and other-approach predicted achievement. The indirect effects of achievement goals through emotion variables on achievement were assessed using bias-corrected bootstrapping. No mediation effects were found. Implications for educational practice are discussed. PMID:27199836

  9. Implementation of a parallel-beam optical-CT apparatus for three-dimensional radiation dosimetry using a high-resolution CCD camera

    NASA Astrophysics Data System (ADS)

    Huang, Wen-Tzeng; Chen, Chin-Hsing; Hung, Chao-Nan; Tuan, Chiu-Ching; Chang, Yuan-Jen

    2015-06-01

    In this study, a charge-coupled device (CCD) camera with 2-megapixel (1920×1080-pixel) and 12-bit resolution was developed for optical computed tomography(optical CT). The signal-to-noise ratio (SNR) of our system was 30.12 dB, better than that of commercially available CCD cameras (25.31 dB). The 50% modulation transfer function (MTF50) of our 1920×1080-pixel camera gave a line width per picture height (LW/PH) of 745, which is 73% of the diffraction-limited resolution. Compared with a commercially available 1-megapixel CCD camera (1296×966-pixel) with a LW/PH=358 and 46.6% of the diffraction-limited resolution, our camera system provided higher spatial resolution and better image quality. The NIPAM gel dosimeter was used to evaluate the optical CT with a 2-megapixel CCD. A clinical five-field irradiation treatment plan was generated using the Eclipse planning system (Varian Corp., Palo Alto, CA, USA). The gel phantom was irradiated using a 6-MV Varian Clinac IX linear accelerator (Varian). The measured NIPAM gel dose distributions and the calculated dose distributions, generated by the treatment planning software (TPS), were compared using the 3% dose-difference and 3 mm distance-to-agreement criteria. The gamma pass rate was as high as 98.2% when 2-megapixel CCD camera was used in optical CT. However, the gamma pass rate was only 96.0% when a commercially available 1-megapixel CCD camera was used.

  10. Iterative Algorithms for Ptychographic Phase Retrieval

    SciTech Connect

    Yang, Chao; Qian, Jianliang; Schirotzek, Andre; Maia, Filipe; Marchesini, Stefano

    2011-05-03

    Ptychography promises diffraction limited resolution without the need for high resolution lenses. To achieve high resolution one has to solve the phase problem for many partially overlapping frames. Here we review some of the existing methods for solving ptychographic phase retrieval problem from a numerical analysis point of view, and propose alternative methods based on numerical optimization.

  11. Effects on High School Students of Conflict Resolution Training Integrated into English Literature.

    ERIC Educational Resources Information Center

    Stevahn, Laurie; Johnson, David W.; Johnson, Roger T.; Green, Kathy; Laginski, Anne Marie

    1997-01-01

    Examines the effectiveness of a conflict-resolution program in raising the academic achievement of a suburban Canadian high school English class. Argues that the conflict-resolution training raised academic performance at the same time that it taught conflict-resolution techniques. Suggests incorporating conflict-resolution training into core high…

  12. Super-resolution imaging in live cells.

    PubMed

    Cox, Susan

    2015-05-01

    Over the last twenty years super-resolution fluorescence microscopy has gone from proof-of-concept experiments to commercial systems being available in many labs, improving the resolution achievable by up to a factor of 10 or more. There are three major approaches to super-resolution, stimulated emission depletion microscopy, structured illumination microscopy, and localisation microscopy, which have all produced stunning images of cellular structures. A major current challenge is optimising performance of each technique so that the same sort of data can be routinely taken in live cells. There are several major challenges, particularly phototoxicity and the speed with which images of whole cells, or groups of cells, can be acquired. In this review we discuss the various approaches which can be successfully used in live cells, the tradeoffs in resolution, speed, and ease of implementation which one must make for each approach, and the quality of results that one might expect from each technique.

  13. High resolution in galaxy photometry and imaging

    NASA Astrophysics Data System (ADS)

    Nieto, J.-L.; Lelievre, G.

    Techniques for increasing the resolution of ground-based photometric observations of galaxies are discussed. The theoretical limitations on resolution and their implications for choosing telescope size at a given site considered, with an emphasis on the importance of the Fried (1966) parameter r0. The techniques recommended are shortening exposure time, selection of the highest-resolution images, and a posteriori digital image processing (as opposed to active-mirror image stabilization or the cine-CCD system of Fort et al., 1984). The value of the increased resolution (by a factor of 2) achieved at Pic du Midi observatory for studies of detailed structure in extragalactic objects, for determining the distance to galaxies, and for probing the central cores of galaxies is indicated.

  14. Invariant high resolution optical skin imaging

    NASA Astrophysics Data System (ADS)

    Murali, Supraja; Rolland, Jannick

    2007-02-01

    Optical Coherence Microscopy (OCM) is a bio-medical low coherence interferometric imaging technique that has become a topic of active research because of its ability to provide accurate, non-invasive cross-sectional images of biological tissue with much greater resolution than the current common technique ultrasound. OCM is a derivative of Optical Coherence Tomography (OCT) that enables greater resolution imposed by the implementation of an optical confocal design involving high numerical aperture (NA) focusing in the sample. The primary setback of OCM, however is the depth dependence of the lateral resolution obtained that arises from the smaller depth of focus of the high NA beam. We propose to overcome this limitation using a dynamic focusing lens design that can achieve quasi-invariant lateral resolution up to 1.5mm depth of skin tissue.

  15. Automated conflict resolution issues

    NASA Technical Reports Server (NTRS)

    Wike, Jeffrey S.

    1991-01-01

    A discussion is presented of how conflicts for Space Network resources should be resolved in the ATDRSS era. The following topics are presented: a description of how resource conflicts are currently resolved; a description of issues associated with automated conflict resolution; present conflict resolution strategies; and topics for further discussion.

  16. Video resolution enhancement

    NASA Astrophysics Data System (ADS)

    Schultz, Richard R.; Stevenson, Robert L.

    1995-03-01

    With the advent of High Definition Television, it will become desirable to convert existing video sequence data into higher-resolution formats. This conversion process already occurs within the human visual system to some extent, since the perceived spatial resolution of a sequence appears much higher than the actual spatial resolution of an individual frame. This paper addresses how to utilize both the spatial and temporal information present in a sequence in order to generate high-resolution video. A novel observation model based on motion compensated subsampling is proposed for a video sequence. Since the reconstruction problem is ill-posed, Bayesian restoration with a discontinuity-preserving prior image model is used to extract high-resolution image sequences will be shown, with dramatic improvements provided over various single frame interpolation methods.

  17. The Mechanics of Human Achievement.

    PubMed

    Duckworth, Angela L; Eichstaedt, Johannes C; Ungar, Lyle H

    2015-07-01

    Countless studies have addressed why some individuals achieve more than others. Nevertheless, the psychology of achievement lacks a unifying conceptual framework for synthesizing these empirical insights. We propose organizing achievement-related traits by two possible mechanisms of action: Traits that determine the rate at which an individual learns a skill are talent variables and can be distinguished conceptually from traits that determine the effort an individual puts forth. This approach takes inspiration from Newtonian mechanics: achievement is akin to distance traveled, effort to time, skill to speed, and talent to acceleration. A novel prediction from this model is that individual differences in effort (but not talent) influence achievement (but not skill) more substantially over longer (rather than shorter) time intervals. Conceptualizing skill as the multiplicative product of talent and effort, and achievement as the multiplicative product of skill and effort, advances similar, but less formal, propositions by several important earlier thinkers.

  18. The Mechanics of Human Achievement

    PubMed Central

    Duckworth, Angela L.; Eichstaedt, Johannes C.; Ungar, Lyle H.

    2015-01-01

    Countless studies have addressed why some individuals achieve more than others. Nevertheless, the psychology of achievement lacks a unifying conceptual framework for synthesizing these empirical insights. We propose organizing achievement-related traits by two possible mechanisms of action: Traits that determine the rate at which an individual learns a skill are talent variables and can be distinguished conceptually from traits that determine the effort an individual puts forth. This approach takes inspiration from Newtonian mechanics: achievement is akin to distance traveled, effort to time, skill to speed, and talent to acceleration. A novel prediction from this model is that individual differences in effort (but not talent) influence achievement (but not skill) more substantially over longer (rather than shorter) time intervals. Conceptualizing skill as the multiplicative product of talent and effort, and achievement as the multiplicative product of skill and effort, advances similar, but less formal, propositions by several important earlier thinkers. PMID:26236393

  19. Super-resolution imaging of the bacterial division machinery.

    PubMed

    Buss, Jackson; Coltharp, Carla; Xiao, Jie

    2013-01-21

    Bacterial cell division requires the coordinated assembly of more than ten essential proteins at midcell. Central to this process is the formation of a ring-like suprastructure (Z-ring) by the FtsZ protein at the division plan. The Z-ring consists of multiple single-stranded FtsZ protofilaments, and understanding the arrangement of the protofilaments inside the Z-ring will provide insight into the mechanism of Z-ring assembly and its function as a force generator. This information has remained elusive due to current limitations in conventional fluorescence microscopy and electron microscopy. Conventional fluorescence microscopy is unable to provide a high-resolution image of the Z-ring due to the diffraction limit of light (~200 nm). Electron cryotomographic imaging has detected scattered FtsZ protofilaments in small C. crescentus cells, but is difficult to apply to larger cells such as E. coli or B. subtilis. Here we describe the application of a super-resolution fluorescence microscopy method, Photoactivated Localization Microscopy (PALM), to quantitatively characterize the structural organization of the E. coli Z-ring. PALM imaging offers both high spatial resolution (~35 nm) and specific labeling to enable unambiguous identification of target proteins. We labeled FtsZ with the photoactivatable fluorescent protein mEos2, which switches from green fluorescence (excitation = 488 nm) to red fluorescence (excitation = 561 nm) upon activation at 405 nm. During a PALM experiment, single FtsZ-mEos2 molecules are stochastically activated and the corresponding centroid positions of the single molecules are determined with <20 nm precision. A super-resolution image of the Z-ring is then reconstructed by superimposing the centroid positions of all detected FtsZ-mEos2 molecules. Using this method, we found that the Z-ring has a fixed width of ~100 nm and is composed of a loose bundle of FtsZ protofilaments that overlap with each other in three dimensions. These data provide

  20. Improved reduced-resolution satellite imagery

    NASA Technical Reports Server (NTRS)

    Ellison, James; Milstein, Jaime

    1995-01-01

    The resolution of satellite imagery is often traded-off to satisfy transmission time and bandwidth, memory, and display limitations. Although there are many ways to achieve the same reduction in resolution, algorithms vary in their ability to preserve the visual quality of the original imagery. These issues are investigated in the context of the Landsat browse system, which permits the user to preview a reduced resolution version of a Landsat image. Wavelets-based techniques for resolution reduction are proposed as alternatives to subsampling used in the current system. Experts judged imagery generated by the wavelets-based methods visually superior, confirming initial quantitative results. In particular, compared to subsampling, the wavelets-based techniques were much less likely to obscure roads, transmission lines, and other linear features present in the original image, introduce artifacts and noise, and otherwise reduce the usefulness of the image. The wavelets-based techniques afford multiple levels of resolution reduction and computational speed. This study is applicable to a wide range of reduced resolution applications in satellite imaging systems, including low resolution display, spaceborne browse, emergency image transmission, and real-time video downlinking.

  1. A high-resolution time-to-digital converter using a three-level resolution

    NASA Astrophysics Data System (ADS)

    Dehghani, Asma; Saneei, Mohsen; Mahani, Ali

    2016-08-01

    In this article, a three-level resolution Vernier delay line time-to-digital converter (TDC) was proposed. The proposed TDC core was based on the pseudo-differential digital architecture that made it insensitive to nMOS and pMOS transistor mismatches. It also employed a Vernier delay line (VDL) in conjunction with an asynchronous read-out circuitry. The time interval resolution was equal to the difference of delay between buffers of upper and lower chains. Then, via the extra chain included in the lower delay line, resolution was controlled and power consumption was reduced. This method led to high resolution and low power consumption. The measurement results of TDC showed a resolution of 4.5 ps, 12-bit output dynamic range, and integral nonlinearity of 1.5 least significant bits. This TDC achieved the consumption of 68.43 µW from 1.1-V supply.

  2. Improved Imaging Resolution in Desorption Electrospray Ionization Mass Spectrometry

    SciTech Connect

    Kertesz, Vilmos; Van Berkel, Gary J

    2008-01-01

    Imaging resolution of desorption electrospray ionization mass spectrometry (DESI-MS) was investigated using printed patterns on paper and thin-layer chromatography (TLC) plate surfaces. Resolution approaching 40 m was achieved with a typical DESI-MS setup, which is approximately 5 times better than the best resolution reported previously. This improvement was accomplished with careful control of operational parameters (particularly spray tip-to-surface distance, solvent flow rate, and spacing of lane scans). Also, an appropriately strong analyte/surface interaction and uniform surface texture on the size scale no larger that the desired imaging resolution were required to achieve this resolution. Overall, conditions providing the smallest possible effective desorption/ionization area in the DESI impact plume region and minimizing the analyte redistribution on the surface during analysis led to the improved DESI-MS imaging resolution.

  3. Design and implementation of a Cooke triplet based wave-front coded super-resolution imaging system

    NASA Astrophysics Data System (ADS)

    Zhao, Hui; Wei, Jingxuan

    2015-09-01

    Wave-front coding is a powerful technique that could be used to extend the DOF (depth of focus) of incoherent imaging system. It is the suitably designed phase mask that makes the system defocus invariant and it is the de-convolution algorithm that generates the clear image with large DOF. Compared with the traditional imaging system, the point spread function (PSF) in wave-front coded imaging system has quite a large support size and this characteristic makes wave-front coding be capable of realizing super-resolution imaging without replacing the current sensor with one of smaller pitch size. An amplification based single image super-resolution reconstruction procedure has been specifically designed for wave-front coded imaging system and its effectiveness has been demonstrated experimentally. A Cooke Triplet based wave-front coded imaging system is established. For a focal length of 50 mm and f-number 4.5, objects within the range [5 m, ∞] could be clearly imaged, which indicates a DOF extension ratio of approximately 20. At the same time, the proposed processing procedure could produce at least 3× resolution improvement, with the quality of the reconstructed super-resolution image approaching the diffraction limit.

  4. High-resolution parallel optical coherence tomography in scattering samples

    NASA Astrophysics Data System (ADS)

    Laubscher, M.; Ducros, Mathieu G.; Karamata, Boris; Bourquin, Stephane; Lasser, Theo

    2001-11-01

    Parallel optical coherence tomography in scattering samples is demonstrated using a 58 by 58 smart-pixel detector array. A femtosecond mode-locked Ti:Sapphire laser in combination with a free space Michelson interferometer was employed to achieve 4micrometers longitudinal resolution and 9mm transverse resolution on a 260x260 micrometers 2 field of view. We imaged a resolution target covered by an intralipid solution with different scattering coefficients as well as onion cells.

  5. Projection based image restoration, super-resolution and error correction codes

    NASA Astrophysics Data System (ADS)

    Bauer, Karl Gregory

    Super-resolution is the ability of a restoration algorithm to restore meaningful spatial frequency content beyond the diffraction limit of the imaging system. The Gerchberg-Papoulis (GP) algorithm is one of the most celebrated algorithms for super-resolution. The GP algorithm is conceptually simple and demonstrates the importance of using a priori information in the formation of the object estimate. In the first part of this dissertation the continuous GP algorithm is discussed in detail and shown to be a projection on convex sets algorithm. The discrete GP algorithm is shown to converge in the exactly-, over- and under-determined cases. A direct formula for the computation of the estimate at the kth iteration and at convergence is given. This analysis of the discrete GP algorithm sets the stage to connect super-resolution to error-correction codes. Reed-Solomon codes are used for error-correction in magnetic recording devices, compact disk players and by NASA for space communications. Reed-Solomon codes have a very simple description when analyzed with the Fourier transform. This signal processing approach to error- correction codes allows the error-correction problem to be compared with the super-resolution problem. The GP algorithm for super-resolution is shown to be equivalent to the correction of errors with a Reed-Solomon code over an erasure channel. The Restoration from Magnitude (RFM) problem seeks to recover a signal from the magnitude of the spectrum. This problem has applications to imaging through a turbulent atmosphere. The turbulent atmosphere causes localized changes in the index of refraction and introduces different phase delays in the data collected. Synthetic aperture radar (SAR) and hyperspectral imaging systems are capable of simultaneously recording multiple images of different polarizations or wavelengths. Each of these images will experience the same turbulent atmosphere and have a common phase distortion. A projection based restoration

  6. EDUCATIONAL ACHIEVEMENT AND THE NAVAJO.

    ERIC Educational Resources Information Center

    HAAS, JOHN; MELVILLE, ROBERT

    A STUDY WAS DEVISED TO APPRAISE THE ACADEMIC ACHIEVEMENT OF NAVAJO STUDENTS LIVING IN DORMITORIES AWAY FROM THE INDIAN RESERVATION. THE FOLLOWING SEVEN FACTORS WERE CHOSEN TO BE INVESTIGATED AS BEING DIRECTLY RELATED TO ACHIEVEMENT--(1) INTELLIGENCE, (2) READING ABILITY, (3) ANXIETY, (4) SELF-CONCEPT, (5) MOTIVATION, (6) VERBAL DEVELOPMENT, (7)…

  7. Sociocultural Origins of Achievement Motivation

    ERIC Educational Resources Information Center

    Maehr, Martin L.

    1977-01-01

    Presents a theoretical review of work on sociocultural influences on achievement, focusing on a critical evaluation of the work of David McClellan. Offers an alternative conception of achievement motivation which stresses the role of contextual and situational factors in addition to personality factors. Available from: Transaction Periodicals…

  8. Raising Boys' Achievement in Schools.

    ERIC Educational Resources Information Center

    Bleach, Kevan, Ed.

    This book offers insights into the range of strategies and good practice being used to raise the achievement of boys. Case studies by school-based practitioners suggest ideas and measures to address the issue of achievement by boys. The contributions are: (1) "Why the Likely Lads Lag Behind" (Kevan Bleach); (2) "Helping Boys Do…

  9. Teaching the Low Level Achiever.

    ERIC Educational Resources Information Center

    Salomone, Ronald E., Ed.

    1986-01-01

    Intended for teachers of the English language arts, the articles in this issue offer suggestions and techniques for teaching the low level achiever. Titles and authors of the articles are as follows: (1) "A Point to Ponder" (Rachel Martin); (2) "Tracking: A Self-Fulfilling Prophecy of Failure for the Low Level Achiever" (James Christopher Davis);…

  10. Early Intervention and Student Achievement

    ERIC Educational Resources Information Center

    Hormes, Mridula T.

    2009-01-01

    The United States Department of Education has been rigorous in holding all states accountable with regard to student achievement. The No Child Left Behind Act of 2001 clearly laid out federal mandates for all schools to follow. K-12 leaders of public schools are very aware of the fact that results in terms of student achievement need to improve…

  11. Parental Involvement and Academic Achievement

    ERIC Educational Resources Information Center

    Goodwin, Sarah Christine

    2015-01-01

    This research study examined the correlation between student achievement and parent's perceptions of their involvement in their child's schooling. Parent participants completed the Parent Involvement Project Parent Questionnaire. Results slightly indicated parents of students with higher level of achievement perceived less demand or invitations…

  12. Asperger Syndrome and Academic Achievement.

    ERIC Educational Resources Information Center

    Griswold, Deborah E.; Barnhill, Gena P.; Myles, Brenda Smith; Hagiwara, Taku; Simpson, Richard L.

    2002-01-01

    A study focused on identifying the academic characteristics of 21 children and youth who have Asperger syndrome. Students had an extraordinary range of academic achievement scores, extending from significantly above average to far below grade level. Lowest achievement scores were shown for numerical operations, listening comprehension, and written…

  13. Perils of Standardized Achievement Testing

    ERIC Educational Resources Information Center

    Haladyna, Thomas M.

    2006-01-01

    This article argues that the validity of standardized achievement test-score interpretation and use is problematic; consequently, confidence and trust in such test scores may often be unwarranted. The problem is particularly severe in high-stakes situations. This essay provides a context for understanding standardized achievement testing, then…

  14. Stress Correlates and Academic Achievement.

    ERIC Educational Resources Information Center

    Bentley, Donna Anderson; And Others

    An ongoing concern for educators is the identification of factors that contribute to or are associated with academic achievement; one such group of variables that has received little attention are those involving stress. The relationship between perceived sources of stress and academic achievement was examined to determine if reactions to stress…

  15. School Size and Student Achievement

    ERIC Educational Resources Information Center

    Riggen, Vicki

    2013-01-01

    This study examined whether a relationship between high school size and student achievement exists in Illinois public high schools in reading and math, as measured by the Prairie State Achievement Exam (PSAE), which is administered to all Illinois 11th-grade students. This study also examined whether the factors of socioeconomic status, English…

  16. School-Community Relations, Community Support, and Student Achievement: A Summary of Findings.

    ERIC Educational Resources Information Center

    Bowles, B. Dean

    Summaries of four studies on school community relations, student achievement and community support are provided in this report. The studies abstracted are: (1) "School-Community Relations, Student Achievement, and Conflict Resolution," by John E. Ingram, Jr.; (2) "School-Community Relations and Student Achievement in Communities of Differing…

  17. High-Resolution PET Detector. Final report

    SciTech Connect

    Karp, Joel

    2014-03-26

    The objective of this project was to develop an understanding of the limits of performance for a high resolution PET detector using an approach based on continuous scintillation crystals rather than pixelated crystals. The overall goal was to design a high-resolution detector, which requires both high spatial resolution and high sensitivity for 511 keV gammas. Continuous scintillation detectors (Anger cameras) have been used extensively for both single-photon and PET scanners, however, these instruments were based on NaI(Tl) scintillators using relatively large, individual photo-multipliers. In this project we investigated the potential of this type of detector technology to achieve higher spatial resolution through the use of improved scintillator materials and photo-sensors, and modification of the detector surface to optimize the light response function.We achieved an average spatial resolution of 3-mm for a 25-mm thick, LYSO continuous detector using a maximum likelihood position algorithm and shallow slots cut into the entrance surface.

  18. Quantifying intraocular scatter with near diffraction-limited double-pass point spread function

    PubMed Central

    Zhao, Junlei; Xiao, Fei; Kang, Jian; Zhao, Haoxin; Dai, Yun; Zhang, Yudong

    2016-01-01

    Measurement of the double-pass (DP) point-spread function (PSF) can provide an objective and non-invasive method for estimating intraocular scatter in the human eye. The objective scatter index (OSI), which is calculated from the DP PSF images, is commonly used to quantify intraocular scatter. In this article, we simulated the effect of higher-order ocular aberrations on OSI, and the results showed that higher-order ocular aberrations had a significant influence on OSI. Then we developed an adaptive optics DP PSF measurement system (AO-DPPMS) which was capable of correcting ocular aberrations up to eighth-order radial Zernike modes over a 6.0-mm pupil. Employing this system, we obtained DP PSF images of four subjects at the fovea. OSI values with aberrations corrected up to 2nd, 5th and 8th Zernike order were calculated respectively, from the DP PSF images of the four subjects. The experimental results were consistent with the simulation, suggesting that it is necessary to compensate for the higher-order ocular aberrations for accurate intraocular scatter estimation. PMID:27895998

  19. All-fiber-integrated single frequency tapered fiber amplifier with near diffraction limited output

    NASA Astrophysics Data System (ADS)

    Zhou, Zichao; Zhang, Hanwei; Wang, Xiaolin; Pan, Zhiyong; Su, Rongtao; Yang, Baolai; Zhou, Pu; Xu, Xiaojun

    2016-06-01

    We present an all-fiber single frequency high-power amplifier using tapered ytterbium-doped fiber (T-YDF) based on a master oscillator power amplification (MOPA) scheme. Different from previous laser amplifiers, the monolithic system is all-fiber-integrated, employing a large mode area (LMA) T-YDF and co-pump scheme. The LMA T-YDF is 7 m long and its core/inner cladding diameters are 20.4/237.1 μm and 46.9/579.9 μm in the input port and output port, respectively. In experiment, the laser amplifier is shown to generate up to 53 W of single frequency laser with slope efficiency of 57.7%, which indicates more than a two times increase of the stimulated Brillouin scattering (SBS) threshold than common LMA fibers with core/inner cladding diameters of 20/400 μm. At the highest output power, the M 2 factor is measured to be 1.25 and 1.20 in the X and Y directions. Results show that this T-YDF can be scaled up to even higher power when other SBS suppression methods are employed simultaneously.

  20. Beat the diffraction limit in 3D direct laser writing in photosensitive glass.

    PubMed

    Bellec, Matthieu; Royon, Arnaud; Bousquet, Bruno; Bourhis, Kevin; Treguer, Mona; Cardinal, Thierry; Richardson, Martin; Canioni, Lionel

    2009-06-08

    Three-dimensional (3D) femtosecond laser direct structuring in transparent materials is widely used for photonic applications. However, the structure size is limited by the optical diffraction. Here we report on a direct laser writing technique that produces subwavelength nanostructures independently of the experimental limiting factors. We demonstrate 3D nanostructures of arbitrary patterns with feature sizes down to 80 nm, less than one tenth of the laser processing wavelength. Its ease of implementation for novel nanostructuring, with its accompanying high precision will open new opportunities for the fabrication of nanostructures for plasmonic and photonic devices and for applications in metamaterials.

  1. Conventional fluorescence microscopy below the diffraction limit with simultaneous capture of two fluorophores in DNA origami

    NASA Astrophysics Data System (ADS)

    Glasgow, Ben J.

    2016-02-01

    A conventional fluorescence microscope was previously constructed for simultaneous imaging of two colors to gain sub-diffraction localization. The system is predicated on color separation of overlapping Airy discs, construction of matrices of Cartesian coordinates to determine locations as well as centers of the point spread functions of fluorophores. Quantum dots that are separated by as little as 10 nm were resolved in the x-y coordinates. Inter-fluorophore distances that vary by 10 nm could also be distinguished. Quantum dots are bright point light source emitters that excite with a single laser and can serve as a label for many biomolecules. Here, alterations in the method are described to test the ability to resolve Atto 488 and Atto 647 dyes attached to DNA origami at ~40 nm spacing intervals. Dual laser excitation is used in tandem with multi-wavelength bandpass filters. Notwithstanding challenges from reduced intensity in Atto labeled DNA origami helical bundles compared to quantum dots, preliminary data show a mean inter-fluorophore distance of 56 nm with a range (14-148 nm). The range closely matches published results with DNA origami with other methods of subdiffraction microscopy. Sub-diffraction simultaneous two-color imaging fluorescence microscopy acronymically christened (SSTIFM) is a simple, readily accessible, technique for measurement of inter-fluorophore distances in compartments less than 40 nm. Preliminary results with so called nanorulers are encouraging for use with other biomolecules.

  2. Real-time dynamic coupling of GPC-enhanced diffraction-limited focal spots

    NASA Astrophysics Data System (ADS)

    Villangca, Mark; Bañas, Andrew; Kopylov, Oleksii; Palima, Darwin; Glückstad, Jesper

    2015-03-01

    We have previously demonstrated on-demand dynamic coupling of an optically manipulated wave-guided optical waveguide (WOW) using diffractive techniques on a "point and shoot" approach. In this work, the generation of the coupling focal spots is done in real-time following the position of the WOW. Object-tracking routine has been added in the trapping program to get the position of the WOW. This approach allows continuous coupling of light through the WOWs which may be useful in some application. In addition, we include a GPC light shaper module in the holography setup to efficiently illuminate the spatial light modulator (SLM). The ability to switch from on-demand to continuous addressing with efficient illumination leverages our WOWs for potential applications in stimulation and nonlinear optics.

  3. Conventional fluorescence microscopy below the diffraction limit with simultaneous capture of two fluorophores in DNA origami

    PubMed Central

    2016-01-01

    A conventional fluorescence microscope was previously constructed for simultaneous imaging of two colors to gain subdiffraction localization. The system is predicated on color separation of overlapping Airy discs, construction of matrices of Cartesian coordinates to determine locations as well as centers of the point spread functions of fluorophores. Quantum dots that are separated by as little as 10 nm were resolved in the x-y coordinates. Inter-fluorophore distances that vary by 10 nm could also be distinguished. Quantum dots are bright point light source emitters that excite with a single laser and can serve as a label for many biomolecules. Here, alterations in the method are described to test the ability to resolve Atto 488 and Atto 647 dyes attached to DNA origami at ~40 nm spacing intervals. Dual laser excitation is used in tandem with multi-wavelength bandpass filters. Notwithstanding challenges from reduced intensity in Atto labeled DNA origami helical bundles compared to quantum dots, preliminary data show a mean inter-fluorophore distance of 56 nm with a range (14-148 nm). The range closely matches published results with DNA origami with other methods of subdiffraction microscopy. Sub-diffraction simultaneous two-color imaging fluorescence microscopy acronymically christened (SSTIFM) is a simple, readily accessible, technique for measurement of inter-fluorophore distances in compartments less than 40 nm. Preliminary results with so called nanorulers are encouraging for use with other biomolecules. PMID:27307653

  4. Space infrared telescope facility wide field and diffraction limited array camera (IRAC)

    NASA Technical Reports Server (NTRS)

    Fazio, G. G.

    1986-01-01

    IRAC focal plane detector technology was developed and studies of alternate focal plane configurations were supported. While any of the alternate focal planes under consideration would have a major impact on the Infrared Array Camera, it was possible to proceed with detector development and optical analysis research based on the proposed design since, to a large degree, the studies undertaken are generic to any SIRTF imaging instrument. Development of the proposed instrument was also important in a situation in which none of the alternate configurations has received the approval of the Science Working Group.

  5. A Monte Carlo approach to tolerance analysis of a near-diffraction limited optical imaging system

    NASA Technical Reports Server (NTRS)

    Davenport, W. H.

    1980-01-01

    A computer program was developed to generate random numbers on the Sigma V computer. A subroutine gave a random real number from a uniform distribution on the interval (0,1). A normal distribution with mean zero and variance 1 was also obtained.

  6. Design of compact apochromatic lens with very-broad spectrum and high resolution

    NASA Astrophysics Data System (ADS)

    Yan, Aqi; Cao, Jianzhong; Zhang, Jian; Zhang, Zhi; Wang, Hao; Wu, Dengshan; Zhou, Zuofeng; Zhang, Kaisheng; Lei, Yangjie

    2013-09-01

    This paper designs a compact apochromatic lens with long focal length, which operates over very-broad spectrum from 400nm to 900nm for high resolution image application. The focal length is 290mm, and F-number is 4.5.In order to match CCD sensor, lens resolution must be higher than 100lp/mm. It is a significant challenge to correct secondary spectrum over very-broad spectrum for this application. The paper firstly pays much attention on dispersion characteristic of optical materials over this very-broad spectrum, and dispersion characteristic of glasses is analyzed. After properly glasses combinations and optimal lens structure selected, this compact apochromatic lens is designed. The lens described in this paper comprises fewer lenses, most of them are ordinary optical materials, and only one special flint type TF3 with anomalous dispersion properties is used for secondary spectrum correction. Finally, the paper shows MTF and aberration curve for performance evaluation. It can be seen that MTF of the designed lens nearly reach diffraction limit at Nyquist frequency 100lp/mm, and residual secondary spectrum is greatly reduced to less than 0.03mm (in the lines 550nm and 787.5nm). The overall length of this compact apochromatic lens is just 0.76 times its focal length, and because of fewer lenses and ordinary optical materials widely used, production cost is also greatly reduced.

  7. Super-Resolution Microscopy of Cerebrospinal Fluid Biomarkers as a Tool for Alzheimer's Disease Diagnostics.

    PubMed

    Zhang, William I; Antonios, Gregory; Rabano, Alberto; Bayer, Thomas A; Schneider, Anja; Rizzoli, Silvio O

    2015-01-01

    Alzheimer's disease (AD) is neuropathologically characterized by aggregates of amyloid-β peptides (Aβ) and tau proteins. The consensus in the AD field is that Aβ and tau should serve as diagnostic biomarkers for AD. However, their aggregates have been difficult to investigate by conventional fluorescence microscopy, since their size is below the diffraction limit (∼200 nm). To solve this, we turned to a super-resolution imaging technique, stimulated emission depletion (STED) microscopy, which has a high enough precision to allow the discrimination of low- and high-molecular weight aggregates prepared in vitro. We used STED to analyze the structural organization of Aβ and tau in cerebrospinal fluid (CSF) from 36 AD patients, 11 patients with mild cognitive impairment (MCI), and 21 controls. We measured the numbers of aggregates in the CSF samples, and the aggregate sizes and intensities. These parameters enabled us to distinguish AD patients from controls with a specificity of ∼87% and a sensitivity of ∼79% . In addition, the aggregate parameters determined with STED microscopy correlated with the severity of cognitive impairment in AD patients. Finally, these parameters may be useful as predictive tools for MCI cases. The STED parameters of two MCI patients who developed AD during the course of the study, as well as of MCI patients whose Aβ ELISA values fall within the accepted range for AD, placed them close to the AD averages. We suggest that super-resolution imaging is a promising tool for AD diagnostics.

  8. Resolution enhancement of digital laser scanning fluorescence microscopy with a dual-lens optical pickup head

    NASA Astrophysics Data System (ADS)

    Tsai, Rung-Ywan; Chen, Jung-Po; Lee, Yuan-Chin; Chiang, Hung-Chih; Huang, Tai-Ting; Huang, Chun-Chieh; Cheng, Chih-Ming; Cheng, Chung-Ta; Lo, Feng-Hsiang; Tiao, Golden

    2016-10-01

    The resolution of the cell fluorescence image captured by a digital laser scanning microscopy with a modified dual-lens BD-ROM optical pickup head is enhanced by image registration and double sample frequency. A dual objective lens of red (655 nm) and blue (405 or 488 nm) laser sources with numerical apertures of 0.6 and 0.85 is used for sample focusing and position tracking and cell fluorescence image capturing, respectively. The image registration and capturing frequency are based on the address-coded patterns of a sample slide. The address-coded patterns are designed as a string of binary code, which comprises a plurality of base-straight lands and grooves and data-straight grooves. The widths of the base-straight lands, base-straight grooves, and data-straight grooves are 0.38, 0.38, and 0.76 μm, respectively. The numbers of sample signals in the x-direction are measured at every intersection point by intersecting the base intensity of the push-pull signal of the address-coded patterns, which has a minimum spacing of 0.38 μm. After taking a double sample frequency, the resolution of the measured cell fluorescence image is enhanced from 0.38 μm to the diffraction limit of the objective lens.

  9. Super-Resolution Imaging of Plasma Membrane Proteins with Click Chemistry

    PubMed Central

    Mateos-Gil, Pablo; Letschert, Sebastian; Doose, Sören; Sauer, Markus

    2016-01-01

    Besides its function as a passive cell wall, the plasma membrane (PM) serves as a platform for different physiological processes such as signal transduction and cell adhesion, determining the ability of cells to communicate with the exterior, and form tissues. Therefore, the spatial distribution of PM components, and the molecular mechanisms underlying it, have important implications in various biological fields including cell development, neurobiology, and immunology. The existence of confined compartments in the plasma membrane that vary on many length scales from protein multimers to micrometer-size domains with different protein and lipid composition is today beyond all questions. As much as the physiology of cells is controlled by the spatial organization of PM components, the study of distribution, size, and composition remains challenging. Visualization of the molecular distribution of PM components has been impeded mainly due to two problems: the specific labeling of lipids and proteins without perturbing their native distribution and the diffraction-limit of fluorescence microscopy restricting the resolution to about half the wavelength of light. Here, we present a bioorthogonal chemical reporter strategy based on click chemistry and metabolic labeling for efficient and specific visualization of PM proteins and glycans with organic fluorophores in combination with super-resolution fluorescence imaging by direct stochastic optical reconstruction microscopy (dSTORM) with single-molecule sensitivity. PMID:27668214

  10. The Galactic Centre at infrared wavelengths: towards the highest spatial resolution

    NASA Astrophysics Data System (ADS)

    Clénet, Yann; Rouan, Daniel; Léna, Pierre; Gendron, Eric; Lacombe, François

    2007-01-01

    We now know that our Galaxy harbors at its centre a supermassive 3.6×10M black hole. This result came after more than 2 decades of infrared studies of the Galactic Centre and important instrumental developments in infrared detectors and in high spatial resolution techniques. Adaptive optics, which allows diffraction-limited infrared observations and enhanced sensitivity, was actually the major breakthrough in this respect. We introduce in the first section of this article what was our knowledge of the Galactic Centre before the advent of adaptive optics. In the second section, after a reminder of the first adaptive optics observations of this region, we highlight the specificities of Galactic Centre adaptive optics observations. In the third and fourth sections, we present the major results obtained from adaptive optics observations of the Galactic Centre: the case of the supermassive black hole and the paradox of youth. In the fifth section, we introduce two main future facilities that will provide even higher spatial resolutions, Gravity—a second generation VLTI instrument—and Extremely Large Telescopes, and the improvements that we expect with these new instruments in our knowledge of the Galactic Centre region. We conclude in the last section. To cite this article: Y. Clénet et al., C. R. Physique 8 (2007).

  11. Improving resolution in quantum subnanometre-gap tip-enhanced Raman nanoimaging

    PubMed Central

    Zhang, Yingchao; Voronine, Dmitri V.; Qiu, Shangran; Sinyukov, Alexander M.; Hamilton, Mary; Liege, Zachary; Sokolov, Alexei V.; Zhang, Zhenrong; Scully, Marlan O.

    2016-01-01

    Two-dimensional (2D) materials beyond graphene such as transition metal dichalcogenides (TMDs) have unique mechanical, optical and electronic properties with promising applications in flexible devices, catalysis and sensing. Optical imaging of TMDs using photoluminescence and Raman spectroscopy can reveal the effects of structure, strain, doping, edge states, and surface functionalization from materials to bioscience. However, Raman signals are inherently weak and so far have been limited in spatial resolution in TMDs to a few hundred nanometres which is much larger than the intrinsic scale of these effects. Here we overcome the diffraction limit by using resonant tip-enhanced Raman scattering (TERS) of few-layer MoS2, and obtain nanoscale optical images with ~20 nm spatial resolution. This becomes possible due to electric field enhancement in an optimized subnanometre-gap resonant tip-substrate configuration. We investigate the limits of signal enhancement by varying the tip-sample gap with sub-Angstrom precision and observe a quantum quenching behavior, as well as a Schottky-Ohmic transition, for subnanometre gaps, which enable surface mapping based on this new contrast mechanism. This quantum regime of plasmonic gap-mode enhancement with a few nanometre thick MoS2 junction may be used for designing new quantum optoelectronic devices and sensors with a wide range of applications. PMID:27220882

  12. Fine-resolution imaging of solar features using Phase-Diverse Speckle

    NASA Technical Reports Server (NTRS)

    Paxman, Richard G.

    1995-01-01

    Phase-diverse speckle (PDS) is a novel imaging technique intended to overcome the degrading effects of atmospheric turbulence on fine-resolution imaging. As its name suggests, PDS is a blend of phase-diversity and speckle-imaging concepts. PDS reconstructions on solar data were validated by simulation, by demonstrating internal consistency of PDS estimates, and by comparing PDS reconstructions with those produced from well accepted speckle-imaging processing. Several sources of error in data collected with the Swedish Vacuum Solar Telescope (SVST) were simulated: CCD noise, quantization error, image misalignment, and defocus error, as well as atmospheric turbulence model error. The simulations demonstrate that fine-resolution information can be reliably recovered out to at least 70% of the diffraction limit without significant introduction of image artifacts. Additional confidence in the SVST restoration is obtained by comparing its spatial power spectrum with previously-published power spectra derived from both space-based images and earth-based images corrected with traditional speckle-imaging techniques; the shape of the spectrum is found to match well the previous measurements. In addition, the imagery is found to be consistent with, but slightly sharper than, imagery reconstructed with accepted speckle-imaging techniques.

  13. Nanofabrication at 1nm resolution by quantum optical lithography (Presentation Recording)

    NASA Astrophysics Data System (ADS)

    Pavel, Eugen

    2015-08-01

    A major problem in the optical lithography was the diffraction limit. Here, we report and demonstrate a lithography method, Quantum Optical Lithography [1,2], able to attain 1 nm resolution by optical means using new materials (fluorescent photosensitive glass-ceramics and QMC-5 resist). The performance is several times better than that described for any optical or Electron Beam Lithography (EBL) methods. In Fig. 1 we present TEM images of 1 nm lines recorded at 9.6 m/s. a) b) Fig. 1 TEM images of: a) multiple 1 nm lines written in a fluorescent photosensitive glass-ceramics sample; b) single 1 nm line written in QMC-5 resist. References [1] E. Pavel, S. Jinga, B.S. Vasile, A. Dinescu, V. Marinescu, R. Trusca and N. Tosa, "Quantum Optical Lithography from 1 nm resolution to pattern transfer on silicon wafer", Optics and Laser Technology, 60 (2014) 80-84. [2] E. Pavel, S. Jinga, E. Andronescu, B.S. Vasile, G. Kada, A. Sasahara, N. Tosa, A. Matei, M. Dinescu, A. Dinescu and O.R. Vasile, "2 nm Quantum Optical Lithography", Optics Communications,291 (2013) 259-263

  14. Improving resolution in quantum subnanometre-gap tip-enhanced Raman nanoimaging

    NASA Astrophysics Data System (ADS)

    Zhang, Yingchao; Voronine, Dmitri V.; Qiu, Shangran; Sinyukov, Alexander M.; Hamilton, Mary; Liege, Zachary; Sokolov, Alexei V.; Zhang, Zhenrong; Scully, Marlan O.

    2016-05-01

    Two-dimensional (2D) materials beyond graphene such as transition metal dichalcogenides (TMDs) have unique mechanical, optical and electronic properties with promising applications in flexible devices, catalysis and sensing. Optical imaging of TMDs using photoluminescence and Raman spectroscopy can reveal the effects of structure, strain, doping, edge states, and surface functionalization from materials to bioscience. However, Raman signals are inherently weak and so far have been limited in spatial resolution in TMDs to a few hundred nanometres which is much larger than the intrinsic scale of these effects. Here we overcome the diffraction limit by using resonant tip-enhanced Raman scattering (TERS) of few-layer MoS2, and obtain nanoscale optical images with ~20 nm spatial resolution. This becomes possible due to electric field enhancement in an optimized subnanometre-gap resonant tip-substrate configuration. We investigate the limits of signal enhancement by varying the tip-sample gap with sub-Angstrom precision and observe a quantum quenching behavior, as well as a Schottky-Ohmic transition, for subnanometre gaps, which enable surface mapping based on this new contrast mechanism. This quantum regime of plasmonic gap-mode enhancement with a few nanometre thick MoS2 junction may be used for designing new quantum optoelectronic devices and sensors with a wide range of applications.

  15. Improving resolution in quantum subnanometre-gap tip-enhanced Raman nanoimaging.

    PubMed

    Zhang, Yingchao; Voronine, Dmitri V; Qiu, Shangran; Sinyukov, Alexander M; Hamilton, Mary; Liege, Zachary; Sokolov, Alexei V; Zhang, Zhenrong; Scully, Marlan O

    2016-05-25

    Two-dimensional (2D) materials beyond graphene such as transition metal dichalcogenides (TMDs) have unique mechanical, optical and electronic properties with promising applications in flexible devices, catalysis and sensing. Optical imaging of TMDs using photoluminescence and Raman spectroscopy can reveal the effects of structure, strain, doping, edge states, and surface functionalization from materials to bioscience. However, Raman signals are inherently weak and so far have been limited in spatial resolution in TMDs to a few hundred nanometres which is much larger than the intrinsic scale of these effects. Here we overcome the diffraction limit by using resonant tip-enhanced Raman scattering (TERS) of few-layer MoS2, and obtain nanoscale optical images with ~20 nm spatial resolution. This becomes possible due to electric field enhancement in an optimized subnanometre-gap resonant tip-substrate configuration. We investigate the limits of signal enhancement by varying the tip-sample gap with sub-Angstrom precision and observe a quantum quenching behavior, as well as a Schottky-Ohmic transition, for subnanometre gaps, which enable surface mapping based on this new contrast mechanism. This quantum regime of plasmonic gap-mode enhancement with a few nanometre thick MoS2 junction may be used for designing new quantum optoelectronic devices and sensors with a wide range of applications.

  16. A High Resolution Imaging Survey of A Stars with AEOS

    NASA Astrophysics Data System (ADS)

    Patience, J.; Macintosh, B. A.; Max, C. E.

    2001-12-01

    We are conducting a companion search to field and cluster A stars using the U. S. Air Force Advanced Electro-Optical System (AEOS) which includes a 941 actuator adaptive optics system on a 3.7m telescope on Haleakala. Operating at I-band, the diffraction limit of the system is 0".05. This program is designed to address questions in binary star formation and stellar X-ray activity. The first goal of our project is to obtain high resolution adaptive optics observations of a large sample of early-type stars in order to extend the mass range covered by binary star searches and to test binary formation theories. In addition to providing insight into the star formation process, studies of companion stars may also explain the unexpected detection of X-rays from A stars. Since A stars lack both the strong winds of the earlier spectral types and the dynamos of the later spectral types, they are not expected to produce X-rays, however, some A stars are detected as X-ray sources. Typical ROSAT error boxes can include a companion star and the second goal of our program is to investigate the possibility that companions are the true source of X-ray emission from A stars. A stars in the young open clusters Coma Berenices and the Pleiades have been targeted as well as field A stars. Our initial results show a binary fraction for X-ray detected A stars that is twice as high as that of non-detected A stars. We gratefully acknowledge support from the Air Force Office of Scientific Research through grant AFOSR-ISSA-01-NM-016. This work was performed under the auspices of the US Department of Energy by the University of California Lawrence Livermore National Laboratory under contract W-7405-ENG-48.

  17. Lincoln's Spot Resolutions.

    ERIC Educational Resources Information Center

    Mueller, Jean West; Schamel, Wynell Burroughs

    1988-01-01

    Examines the events leading to and immediately following the declaration of war on Mexico in 1846. Includes the second and third pages of Abraham Lincoln's "Spot Resolutions" and presents teaching suggestions for interpreting the document and assessing public opinion. (GEA)

  18. High-Resolution Autoradiography

    NASA Technical Reports Server (NTRS)

    Towe, George C; Gomberg, Henry J; Freemen, J W

    1955-01-01

    This investigation was made to adapt wet-process autoradiography to metallurgical samples to obtain high resolution of segregated radioactive elements in microstructures. Results are confined to development of the technique, which was perfected to a resolution of less than 10 microns. The radioactive samples included carbon-14 carburized iron and steel, nickel-63 electroplated samples, a powder product containing nickel-63, and tungsten-185 in N-155 alloy.

  19. Ultra high resolution tomography

    SciTech Connect

    Haddad, W.S.

    1994-11-15

    Recent work and results on ultra high resolution three dimensional imaging with soft x-rays will be presented. This work is aimed at determining microscopic three dimensional structure of biological and material specimens. Three dimensional reconstructed images of a microscopic test object will be presented; the reconstruction has a resolution on the order of 1000 A in all three dimensions. Preliminary work with biological samples will also be shown, and the experimental and numerical methods used will be discussed.

  20. High Resolution Computed Tomography

    DTIC Science & Technology

    1992-07-31

    samples. 14. SUBJECTTERMS 15. NUMBER OF PAGES 38 High Resolution, Microfocus , Characterization, X - Ray , Micrography, Computed Tomography (CT), Failure...high resolutions (50 g.tm feature sensitivity) when a small field of view (50 mm) is used [11]. Specially designed detectors and a microfocus X - ray ...Wright Laboratories. Feldkamp [14] at Ford used a microfocus X - ray source and an X - ray image intensifier to develop a system capable of 20 g.m