Controlled power delivery for super-resolution imaging of biological samples using digital micromirror device

NASA Astrophysics Data System (ADS)

Valiya Peedikakkal, Liyana; Cadby, Ashley

2017-02-01

Localization based super resolution images of a biological sample is generally achieved by using high power laser illumination with long exposure time which unfortunately increases photo-toxicity of a sample, making super resolution microscopy, in general, incompatible with live cell imaging. Furthermore, the limitation of photobleaching reduces the ability to acquire time lapse images of live biological cells using fluorescence microscopy. Digital Light Processing (DLP) technology can deliver light at grey scale levels by flickering digital micromirrors at around 290 Hz enabling highly controlled power delivery to samples. In this work, Digital Micromirror Device (DMD) is implemented in an inverse Schiefspiegler telescope setup to control the power and pattern of illumination for super resolution microscopy. We can achieve spatial and temporal patterning of illumination by controlling the DMD pixel by pixel. The DMD allows us to control the power and spatial extent of the laser illumination. We have used this to show that we can reduce the power delivered to the sample to allow for longer time imaging in one area while achieving sub-diffraction STORM imaging in another using higher power densities.

Intravascular photoacoustic imaging at 35 and 80 MHz

PubMed Central

Li, Xiang; Wei, Wei; Shung, K. Kirk

2012-01-01

Abstract. The catheter-based intravascular photoacoustic (IVPA) imaging for diagnosing atherosclerosis, which can provide optical absorption contrast of the arterial wall besides acoustic scattering contrast from the conventional intravascular ultrasound (IVUS) imaging, has been intensively researched recently. The resolution of IVPA is determined by the frequency bandwidth of an ultrasonic transducer. Higher resolution can be achieved by increasing the transducer’s working frequency and bandwidth. We introduce IVPA imaging at 35 and 80 MHz by using newly designed integrated IVUS/IVPA probes. This is the first time IVPA has been achieved as high as 80 MHz. Six-micrometer tungsten wires were imaged to evaluate the probes’ spatial resolutions and beam patterns. Healthy rabbit aorta was imaged in vitro. Imaging results show that IVPA has superior contrast over IVUS in identifying the arterial wall, and IVPA at 80 MHz demonstrates extraordinary resolution (35 μm) compared to 35 MHz. PMID:23224004

Multiband super-resolution imaging of graded-index photonic crystal flat lens

NASA Astrophysics Data System (ADS)

Xie, Jianlan; Wang, Junzhong; Ge, Rui; Yan, Bei; Liu, Exian; Tan, Wei; Liu, Jianjun

2018-05-01

Multiband super-resolution imaging of point source is achieved by a graded-index photonic crystal flat lens. With the calculations of six bands in common photonic crystal (CPC) constructed with scatterers of different refractive indices, it can be found that the super-resolution imaging of point source can be realized by different physical mechanisms in three different bands. In the first band, the imaging of point source is based on far-field condition of spherical wave while in the second band, it is based on the negative effective refractive index and exhibiting higher imaging quality than that of the CPC. However, in the fifth band, the imaging of point source is mainly based on negative refraction of anisotropic equi-frequency surfaces. The novel method of employing different physical mechanisms to achieve multiband super-resolution imaging of point source is highly meaningful for the field of imaging.

Focal Plane Detectors for the Advanced Gamma-Ray Imaging System (AGIS)

NASA Astrophysics Data System (ADS)

Wagner, R. G.; Byrum, K.; Drake, G.; Funk, S.; Otte, N.; Smith, A.; Tajima, H.; Williams, D.

2009-05-01

The Advanced Gamma-Ray Imaging System (AGIS) is a concept for the next generation observatory in ground-based very high energy gamma-ray astronomy. It is being designed to achieve a significant improvement in sensitivity compared to current Imaging Air Cherenkov Telescope (IACT) Arrays. One of the main requirements in order that AGIS fulfills this goal will be to achieve higher angular resolution than current IACTs. Simulations show that a substantial improvement in angular resolution may be achieved if the pixel size is reduced to 0.05 deg, i.e. two to three times smaller than for current IACT cameras. Here we present results from testing of alternatives being considered for AGIS, including both silicon photomultipliers (SiPMs) and multi-anode photomultipliers (MAPMTs).

Hyperlens-array-implemented optical microscopy

NASA Astrophysics Data System (ADS)

Iwanaga, Masanobu

2014-08-01

Limit of resolution of conventional optical microscopes has never reached below 100 nm under visible light illumination. We show that numerically designed high-transmittance hyperlens array (HLA) is implemented in an optical microscope and works in practice for achieving one-shot-recording optical images of in-situ placed objects with sub 50 nm resolution in lateral direction. Direct resolution test employing well-defined nanopatterns proves that the HLA-implemented imaging is super-resolution optical microscopy, which works even under nW/mm2 visible illumination for objects. The HLA implementation makes the resolution of conventional microscopes one-scale higher, leading to the 1/10 illumination wavelength range, that is, mesoscopic range.

A digital gigapixel large-format tile-scan camera.

PubMed

Ben-Ezra, M

2011-01-01

Although the resolution of single-lens reflex (SLR) and medium-format digital cameras has increased in recent years, applications for cultural-heritage preservation and computational photography require even higher resolutions. Addressing this issue, a large-format cameras' large image planes can achieve very high resolution without compromising pixel size and thus can provide high-quality, high-resolution images.This digital large-format tile scan camera can acquire high-quality, high-resolution images of static scenes. It employs unique calibration techniques and a simple algorithm for focal-stack processing of very large images with significant magnification variations. The camera automatically collects overlapping focal stacks and processes them into a high-resolution, extended-depth-of-field image.

Superresolution confocal technology for displacement measurements based on total internal reflection.

PubMed

Kuang, Cuifang; Ali, M Yakut; Hao, Xiang; Wang, Tingting; Liu, Xu

2010-10-01

In order to achieve a higher axial resolution for displacement measurement, a novel method is proposed based on total internal reflection filter and confocal microscope principle. A theoretical analysis of the basic measurement principles is presented. The analysis reveals that the proposed confocal detection scheme is effective in enhancing the resolution of nonlinearity of the reflectance curve greatly. In addition, a simple prototype system has been developed based on the theoretical analysis and a series of experiments have been performed under laboratory conditions to verify the system feasibility, accuracy, and stability. The experimental results demonstrate that the axial resolution in displacement measurements is better than 1 nm in a range of 200 nm which is threefold better than that can be achieved using the plane reflector.

Space telescope scientific instruments

NASA Technical Reports Server (NTRS)

Leckrone, D. S.

1979-01-01

The paper describes the Space Telescope (ST) observatory, the design concepts of the five scientific instruments which will conduct the initial observatory observations, and summarizes their astronomical capabilities. The instruments are the wide-field and planetary camera (WFPC) which will receive the highest quality images, the faint-object camera (FOC) which will penetrate to the faintest limiting magnitudes and achieve the finest angular resolution possible, and the faint-object spectrograph (FOS), which will perform photon noise-limited spectroscopy and spectropolarimetry on objects substantially fainter than those accessible to ground-based spectrographs. In addition, the high resolution spectrograph (HRS) will provide higher spectral resolution with greater photometric accuracy than previously possible in ultraviolet astronomical spectroscopy, and the high-speed photometer will achieve precise time-resolved photometric observations of rapidly varying astronomical sources on short time scales.

Lateral resolution improvement in scanning nonlinear dielectric microscopy by measuring super-higher-order nonlinear dielectric constants

NASA Astrophysics Data System (ADS)

Chinone, N.; Yamasue, K.; Hiranaga, Y.; Honda, K.; Cho, Y.

2012-11-01

Scanning nonlinear dielectric microscopy (SNDM) can be used to visualize polarization distributions in ferroelectric materials and dopant profiles in semiconductor devices. Without using a special sharp tip, we achieved an improved lateral resolution in SNDM through the measurement of super-higher-order nonlinearity up to the fourth order. We observed a multidomain single crystal congruent LiTaO3 (CLT) sample, and a cross section of a metal-oxide-semiconductor (MOS) field-effect-transistor (FET). The imaged domain boundaries of the CLT were narrower in the super-higher-order images than in the conventional image. Compared to the conventional method, the super-higher-order method resolved the more detailed structure of the MOSFET.

A novel approach of an absolute coding pattern based on Hamiltonian graph

NASA Astrophysics Data System (ADS)

Wang, Ya'nan; Wang, Huawei; Hao, Fusheng; Liu, Liqiang

2017-02-01

In this paper, a novel approach of an optical type absolute rotary encoder coding pattern is presented. The concept is based on the principle of the absolute encoder to find out a unique sequence that ensures an unambiguous shaft position of any angular. We design a single-ring and a n-by-2 matrix absolute encoder coding pattern by using the variations of Hamiltonian graph principle. 12 encoding bits is used in the single-ring by a linear array CCD to achieve an 1080-position cycle encoding. Besides, a 2-by-2 matrix is used as an unit in the 2-track disk to achieve a 16-bits encoding pattern by using an area array CCD sensor (as a sample). Finally, a higher resolution can be gained by an electronic subdivision of the signals. Compared with the conventional gray or binary code pattern (for a 2n resolution), this new pattern has a higher resolution (2n*n) with less coding tracks, which means the new pattern can lead to a smaller encoder, which is essential in the industrial production.

The spatial resolution of silicon-based electron detectors in beta-autoradiography.

PubMed

Cabello, Jorge; Wells, Kevin

2010-03-21

Thin tissue autoradiography is an imaging modality where ex-vivo tissue sections are placed in direct contact with autoradiographic film. These tissue sections contain a radiolabelled ligand bound to a specific biomolecule under study. This radioligand emits beta - or beta+ particles ionizing silver halide crystals in the film. High spatial resolution autoradiograms are obtained using low energy radioisotopes, such as (3)H where an intrinsic 0.1-1 microm spatial resolution can be achieved. Several digital alternatives have been presented over the past few years to replace conventional film but their spatial resolution has yet to equal film, although silicon-based imaging technologies have demonstrated higher sensitivity compared to conventional film. It will be shown in this work how pixel size is a critical parameter for achieving high spatial resolution for low energy uncollimated beta imaging. In this work we also examine the confounding factors impeding silicon-based technologies with respect to spatial resolution. The study considers charge diffusion in silicon and detector noise, and this is applied to a range of radioisotopes typically used in autoradiography. Finally an optimal detector geometry to obtain the best possible spatial resolution for a specific technology and a specific radioisotope is suggested.

Toward single electron resolution phonon mediated ionization detectors

NASA Astrophysics Data System (ADS)

Mirabolfathi, Nader; Harris, H. Rusty; Mahapatra, Rupak; Sundqvist, Kyle; Jastram, Andrew; Serfass, Bruno; Faiez, Dana; Sadoulet, Bernard

2017-05-01

Experiments seeking to detect rare event interactions such as dark matter or coherent elastic neutrino nucleus scattering are striving for large mass detectors with very low detection threshold. Using Neganov-Luke phonon amplification effect, the Cryogenic Dark Matter Search (CDMS) experiment is reaching unprecedented RMS resolutions of ∼14 eVee. CDMSlite is currently the most sensitive experiment to WIMPs of mass ∼5 GeV/c2 but is limited in achieving higher phonon gains due to an early onset of leakage current into Ge crystals. The contact interface geometry is particularly weak for blocking hole injection from the metal, and thus a new design is demonstrated that allows high voltage bias via vacuum separated electrode. With an increased bias voltage and a×2 Luke phonon gain, world best RMS resolution of sigma ∼7 eVee for 0.25 kg (d=75 mm, h=1 cm) Ge detectors was achieved. Since the leakage current is a function of the field and the phonon gain is a function of the applied voltage, appropriately robust interface blocking material combined with thicker substrate (25 mm) will reach a resolution of ∼2.8 eVee. In order to achieve better resolution of ∼ eV, we are investigating a layer of insulator between the phonon readout surface and the semiconductor crystals.

Chemical bond imaging using higher eigenmodes of tuning fork sensors in atomic force microscopy

NASA Astrophysics Data System (ADS)

Ebeling, Daniel; Zhong, Qigang; Ahles, Sebastian; Chi, Lifeng; Wegner, Hermann A.; Schirmeisen, André

2017-05-01

We demonstrate the ability of resolving the chemical structure of single organic molecules using non-contact atomic force microscopy with higher normal eigenmodes of quartz tuning fork sensors. In order to achieve submolecular resolution, CO-functionalized tips at low temperatures are used. The tuning fork sensors are operated in ultrahigh vacuum in the frequency modulation mode by exciting either their first or second eigenmode. Despite the high effective spring constant of the second eigenmode (on the order of several tens of kN/m), the force sensitivity is sufficiently high to achieve atomic resolution above the organic molecules. This is observed for two different tuning fork sensors with different tip geometries (small tip vs. large tip). These results represent an important step towards resolving the chemical structure of single molecules with multifrequency atomic force microscopy techniques where two or more eigenmodes are driven simultaneously.

A deterministic compressive sensing model for bat biosonar.

PubMed

Hague, David A; Buck, John R; Bilik, Igal

2012-12-01

The big brown bat (Eptesicus fuscus) uses frequency modulated (FM) echolocation calls to accurately estimate range and resolve closely spaced objects in clutter and noise. They resolve glints spaced down to 2 μs in time delay which surpasses what traditional signal processing techniques can achieve using the same echolocation call. The Matched Filter (MF) attains 10-12 μs resolution while the Inverse Filter (IF) achieves higher resolution at the cost of significantly degraded detection performance. Recent work by Fontaine and Peremans [J. Acoustic. Soc. Am. 125, 3052-3059 (2009)] demonstrated that a sparse representation of bat echolocation calls coupled with a decimating sensing method facilitates distinguishing closely spaced objects over realistic SNRs. Their work raises the intriguing question of whether sensing approaches structured more like a mammalian auditory system contains the necessary information for the hyper-resolution observed in behavioral tests. This research estimates sparse echo signatures using a gammatone filterbank decimation sensing method which loosely models the processing of the bat's auditory system. The decimated filterbank outputs are processed with [script-l](1) minimization. Simulations demonstrate that this model maintains higher resolution than the MF and significantly better detection performance than the IF for SNRs of 5-45 dB while undersampling the return signal by a factor of six.

Intrinsic instability of aberration-corrected electron microscopes.

PubMed

Schramm, S M; van der Molen, S J; Tromp, R M

2012-10-19

Aberration-corrected microscopes with subatomic resolution will impact broad areas of science and technology. However, the experimentally observed lifetime of the corrected state is just a few minutes. Here we show that the corrected state is intrinsically unstable; the higher its quality, the more unstable it is. Analyzing the contrast transfer function near optimum correction, we define an "instability budget" which allows a rational trade-off between resolution and stability. Unless control systems are developed to overcome these challenges, intrinsic instability poses a fundamental limit to the resolution practically achievable in the electron microscope.

High-speed MCP anodes for high time resolution low-energy charged particle spectrometers

NASA Astrophysics Data System (ADS)

Saito, Yoshifumi; Yokota, Shoichiro; Asamura, Kazushi; Krieger, Amanda

2017-02-01

The time resolution of low-energy charged particle measurements is becoming higher and higher. In order to realize high time resolution measurements, a 1-D circular delay line anode has been developed as a high-speed microchannel plate (MCP) anode. The maximum count rate of the 1-D circular delay line anode is around 1 × 107/s/360°, which is much higher than the widely used resistive anode, whose maximum count rate is around 1 × 106/s/360°. In order to achieve much higher speeds, an MCP anode with application-specific integrated circuit (ASIC) has been developed. We have decided to adopt an anode configuration in which a discrete anode is formed on a ceramic substrate, and a bare ASIC chip is installed on the back of the ceramic. It has been found that the anode can detect at a high count rate of 2 × 108/s/360°. Developments in both delay line and discrete anodes, as well as readout electronics, will be reviewed.

Improving the lateral resolution of quartz tuning fork-based sensors in liquid by integrating commercial AFM tips into the fiber end.

PubMed

Gonzalez, Laura; Martínez-Martín, David; Otero, Jorge; de Pablo, Pedro José; Puig-Vidal, Manel; Gómez-Herrero, Julio

2015-01-14

The use of quartz tuning fork sensors as probes for scanning probe microscopy is growing in popularity. Working in shear mode, some methods achieve a lateral resolution comparable with that obtained with standard cantilevered probes, but only in experiments conducted in air or vacuum. Here, we report a method to produce and use commercial AFM tips in electrically driven quartz tuning fork sensors operating in shear mode in a liquid environment. The process is based on attaching a standard AFM tip to the end of a fiber probe which has previously been sharpened. Only the end of the probe is immersed in the buffer solution during imaging. The lateral resolution achieved is about 6 times higher than that of the etched microfiber on its own.

Performance modeling of terahertz (THz) and millimeter waves (mmW) pupil plane imaging

NASA Astrophysics Data System (ADS)

Mohammadian, Nafiseh; Furxhi, Orges; Zhang, Lei; Offermans, Peter; Ghazi, Galia; Driggers, Ronald

2018-05-01

Terahertz- (THz) and millimeter-wave sensors are becoming more important in industrial, security, medical, and defense applications. A major problem in these sensing areas is the resolution, sensitivity, and visual acuity of the imaging systems. There are different fundamental parameters in designing a system that have significant effects on the imaging performance. The performance of THz systems can be discussed in terms of two characteristics: sensitivity and spatial resolution. New approaches for design and manufacturing of THz imagers are a vital basis for developing future applications. Photonics solutions have been at the technological forefront in THz band applications. A single scan antenna does not provide reasonable resolution, sensitivity, and speed. An effective approach to imaging is placing a high-performance antenna in a two-dimensional antenna array to achieve higher radiation efficiency and higher resolution in the imaging systems. Here, we present the performance modeling of a pupil plane imaging system to find the resolution and sensitivity efficiency of the imaging system.

Resolution enhancement of fiber Bragg grating temperature sensor using a cavity ring-down technique

NASA Astrophysics Data System (ADS)

Yarai, Atsushi; Hara, Katsuyuki

2018-02-01

A new technique for enhancing the measurement resolution of a fiber Bragg grating (FBG) temperature sensor is proposed. This technique uses a cavity ring-down approach to amplify optical intensity by accumulating unremarkable intensity changes. A wavelength-stabilized optical pulse with a width of 10 ns rotates several times inside an optical fiber loop that contains a FBG sensor. In other words, the loop system functions as an integrator of slight intensity transition. A temperature resolution of at least 0.02 °C was achieved at 20.0 °C. Resolution with this technique is at least five times higher than previous techniques.

A Novel Azimuth Super-Resolution Method by Synthesizing Azimuth Bandwidth of Multiple Tracks of Airborne Stripmap SAR Data

PubMed Central

Wang, Yan; Li, Jingwen; Sun, Bing; Yang, Jian

2016-01-01

Azimuth resolution of airborne stripmap synthetic aperture radar (SAR) is restricted by the azimuth antenna size. Conventionally, a higher azimuth resolution should be achieved by employing alternate modes that steer the beam in azimuth to enlarge the synthetic antenna aperture. However, if a data set of a certain region, consisting of multiple tracks of airborne stripmap SAR data, is available, the azimuth resolution of specific small region of interest (ROI) can be conveniently improved by a novel azimuth super-resolution method as introduced by this paper. The proposed azimuth super-resolution method synthesize the azimuth bandwidth of the data selected from multiple discontinuous tracks and contributes to a magnifier-like function with which the ROI can be further zoomed in with a higher azimuth resolution than that of the original stripmap images. Detailed derivation of the azimuth super-resolution method, including the steps of two-dimensional dechirping, residual video phase (RVP) removal, data stitching and data correction, is provided. The restrictions of the proposed method are also discussed. Lastly, the presented approach is evaluated via both the single- and multi-target computer simulations. PMID:27304959

Investigation of a Multi-Anode Microchannel Plate PMT for Time-of-Flight PET

NASA Astrophysics Data System (ADS)

Choong, Woon-Seng

2010-10-01

We report on an investigation of a mulit-anode microchannel plate PMT for time-of-flight PET detector modules. The primary advantages of an MCP lie in its excellent timing properties (fast rise time and low transit time spread), compact size, and reasonably large active area, thus making it a good candidate for TOF applications. In addition, the anode can be segmented into an array of collection electrodes with fine pitch to attain good position sensitivity. In this paper, we investigate using the Photonis Planacon MCP-PMT with a pore size of 10 μm to construct a PET detector module, specifically for time-of-flight applications. We measure the single electron response by exciting the Planacon with pulsed laser diode. We also measure the performance of the Planacon as a PET detector by coupling a 4 mm×4 mm×10 mm LSO crystal to individual pixel to study its gain uniformity, energy resolution, and timing resolution. The rise time of the Planacon is 440 ps with pulse duration of about 1 ns. A transit time spread of 120 ps FWHM is achieved. The gain is fairly uniform across the central region of the Planacon, but drops off by as much as a factor of 2.5 around the edges. The energy resolution is fairly uniform across the Planacon with an average value of 18.6 ± 0.7% FWHM. While the average timing resolution of 252 ± 7 ps FWHM is achieved in the central region of the Planacon, it degrades to 280 ± 9 ps FWHM for edge pixels and 316 ± 15 ps FWHM for corner pixels. We compare the results with measurements performed with a fast timing conventional PMT (Hamamatsu R-9800). We find that the R9800, which has significantly higher PDE, has a better timing resolution than the Planacon. Furthermore, we perform detector simulations to calculate the improvement that can be achieved with a higher PDE Planacon. The calculation shows that the Planacon can achieve significantly better timing resolution if it can attain the same PDE as the R-9800, while only a 30% improvement is needed to yield a similar timing resolution as the R-9800.

Example-Based Super-Resolution Fluorescence Microscopy.

PubMed

Jia, Shu; Han, Boran; Kutz, J Nathan

2018-04-23

Capturing biological dynamics with high spatiotemporal resolution demands the advancement in imaging technologies. Super-resolution fluorescence microscopy offers spatial resolution surpassing the diffraction limit to resolve near-molecular-level details. While various strategies have been reported to improve the temporal resolution of super-resolution imaging, all super-resolution techniques are still fundamentally limited by the trade-off associated with the longer image acquisition time that is needed to achieve higher spatial information. Here, we demonstrated an example-based, computational method that aims to obtain super-resolution images using conventional imaging without increasing the imaging time. With a low-resolution image input, the method provides an estimate of its super-resolution image based on an example database that contains super- and low-resolution image pairs of biological structures of interest. The computational imaging of cellular microtubules agrees approximately with the experimental super-resolution STORM results. This new approach may offer potential improvements in temporal resolution for experimental super-resolution fluorescence microscopy and provide a new path for large-data aided biomedical imaging.

Developing terahertz imaging equation and enhancement of the resolution of terahertz images using deconvolution

NASA Astrophysics Data System (ADS)

Ahi, Kiarash; Anwar, Mehdi

2016-04-01

This paper introduces a novel reconstruction approach for enhancing the resolution of the terahertz (THz) images. For this purpose the THz imaging equation is derived. According to our best knowledge we are reporting the first THz imaging equation by this paper. This imaging equation is universal for THz far-field imaging systems and can be used for analyzing, describing and modeling of these systems. The geometry and behavior of Gaussian beams in far-field region imply that the FWHM of the THz beams diverge as the frequencies of the beams decrease. Thus, the resolution of the measurement decreases in lower frequencies. On the other hand, the depth of penetration of THz beams decreases as frequency increases. Roughly speaking beams in sub 1.5 THz, are transmitted into integrated circuit (IC) packages and the similar packaged objects. Thus, it is not possible to use the THz pulse with higher frequencies in order to achieve higher resolution inspection of packaged items. In this paper, after developing the 3-D THz point spread function (PSF) of the scanning THz beam and then the THz imaging equation, THz images are enhanced through deconvolution of the THz PSF and THz images. As a result, the resolution has been improved several times beyond the physical limitations of the THz measurement setup in the far-field region and sub-Nyquist images have been achieved. Particularly, MSE and SSIḾ have been increased by 27% and 50% respectively. Details as small as 0.2 mm were made visible in the THz images which originally reveals no details smaller than 2.2 mm. In other words the resolution of the images has been increased by 10 times. The accuracy of the reconstructed images was proved by high resolution X-ray images.

New developments in fabrication of high-energy-resolution analyzers for inelastic x-ray spectroscopy.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Said, A. H.; Sinn, H.; Divan, R.

2011-05-01

In this work new improvements related to the fabrication of spherical bent analyzers for 1 meV energy-resolution inelastic X-ray scattering spectroscopy are presented. The new method includes the use of a two-dimensional bender to achieve the required radius of curvature for X-ray analyzers. The advantage of this method is the ability to monitor the focus during bending, which leads to higher-efficiency analyzers.

The Advanced Gamma-ray Imaging System (AGIS): Focal Plane Detectors

NASA Astrophysics Data System (ADS)

Mukherjee, Reshmi; Byrum, K.; Drake, G.; Falcone, A.; Funk, S.; Horan, D.; Tajima, H.; Wagner, B.; Williams, D.

2008-04-01

Report of the Focal Plane Instrumentation Working Group, AGIS collaboration: The Advanced Gamma-ray Imaging System (AGIS) is a concept for the next generation instrument in ground-based very high energy gamma-ray astronomy. It has the goal of achieving significant improvement in sensitivity over current experiments. One of the main requirements for AGIS will be to achieve higher angular resolution than current imaging atmospheric Cherenkov telescopes (IACTs). Simulations show that a substantial improvement in angular resolution may be achieved if the pixel size is reduced to 0.05 deg, below that of current IACTs. Reducing the cost per channel and improving reliability and modularity are other important considerations. Here we present several alternatives being considered for AGIS, including both silicon photomultipliers (SiPMs) and multi-anode photomultipliers (MAPMTs) and summarize results from feasibility testing by various AGIS photodetector group members.

Ultra-high spatial resolution multi-energy CT using photon counting detector technology

NASA Astrophysics Data System (ADS)

Leng, S.; Gutjahr, R.; Ferrero, A.; Kappler, S.; Henning, A.; Halaweish, A.; Zhou, W.; Montoya, J.; McCollough, C.

2017-03-01

Two ultra-high-resolution (UHR) imaging modes, each with two energy thresholds, were implemented on a research, whole-body photon-counting-detector (PCD) CT scanner, referred to as sharp and UHR, respectively. The UHR mode has a pixel size of 0.25 mm at iso-center for both energy thresholds, with a collimation of 32 × 0.25 mm. The sharp mode has a 0.25 mm pixel for the low-energy threshold and 0.5 mm for the high-energy threshold, with a collimation of 48 × 0.25 mm. Kidney stones with mixed mineral composition and lung nodules with different shapes were scanned using both modes, and with the standard imaging mode, referred to as macro mode (0.5 mm pixel and 32 × 0.5 mm collimation). Evaluation and comparison of the three modes focused on the ability to accurately delineate anatomic structures using the high-spatial resolution capability and the ability to quantify stone composition using the multi-energy capability. The low-energy threshold images of the sharp and UHR modes showed better shape and texture information due to the achieved higher spatial resolution, although noise was also higher. No noticeable benefit was shown in multi-energy analysis using UHR compared to standard resolution (macro mode) when standard doses were used. This was due to excessive noise in the higher resolution images. However, UHR scans at higher dose showed improvement in multi-energy analysis over macro mode with regular dose. To fully take advantage of the higher spatial resolution in multi-energy analysis, either increased radiation dose, or application of noise reduction techniques, is needed.

Recent technologic advances in multi-detector row cardiac CT.

PubMed

Halliburton, Sandra Simon

2009-11-01

Recent technical advances in multi-detector row CT have resulted in lower radiation dose, improved temporal and spatial resolution, decreased scan time, and improved tissue differentiation. Lower radiation doses have resulted from the use of pre-patient z collimators, the availability of thin-slice axial data acquisition, the increased efficiency of ECG-based tube current modulation, and the implementation of iterative reconstruction algorithms. Faster gantry rotation and the simultaneous use of two x-ray sources have led to improvements in temporal resolution, and gains in spatial resolution have been achieved through application of the flying x-ray focal-spot technique in the z-direction. Shorter scan times have resulted from the design of detector arrays with increasing numbers of detector rows and through the simultaneous use of two x-ray sources to allow higher helical pitch. Some improvement in tissue differentiation has been achieved with dual energy CT. This article discusses these recent technical advances in detail.

Fabrication of High-Resolution Gamma-Ray Metallic Magnetic Calorimeters with Ag:Er Sensor and Thick Electroplated Absorbers

NASA Astrophysics Data System (ADS)

Hummatov, Ruslan; Hall, John A.; Kim, Geon-Bo; Friedrich, Stephan; Cantor, Robin; Boyd, S. T. P.

2018-05-01

We are developing metallic magnetic calorimeters for high-resolution gamma-ray spectroscopy for non-destructive assay of nuclear materials. Absorbers for these higher-energy photons can require substantial thickness to achieve adequate stopping power. We developed a new absorber fabrication process using dry-film photoresists to electroform cantilevered, thick absorbers. Gamma detectors with these absorbers have an energy resolution of 38 eV FWHM at 60 keV. In this report, we summarize modifications to STARCryo's "Delta 1000" process for our devices and describe the new absorber fabrication process.

High-resolution low-dose scanning transmission electron microscopy.

PubMed

Buban, James P; Ramasse, Quentin; Gipson, Bryant; Browning, Nigel D; Stahlberg, Henning

2010-01-01

During the past two decades instrumentation in scanning transmission electron microscopy (STEM) has pushed toward higher intensity electron probes to increase the signal-to-noise ratio of recorded images. While this is suitable for robust specimens, biological specimens require a much reduced electron dose for high-resolution imaging. We describe here protocols for low-dose STEM image recording with a conventional field-emission gun STEM, while maintaining the high-resolution capability of the instrument. Our findings show that a combination of reduced pixel dwell time and reduced gun current can achieve radiation doses comparable to low-dose TEM.

High-resolution imaging of magnetic fields using scanning superconducting quantum interference device (SQUID) microscopy

NASA Astrophysics Data System (ADS)

Fong de Los Santos, Luis E.

Development of a scanning superconducting quantum interference device (SQUID) microscope system with interchangeable sensor configurations for imaging magnetic fields of room-temperature (RT) samples with sub-millimeter resolution. The low-critical-temperature (Tc) niobium-based monolithic SQUID sensor is mounted in the tip of a sapphire rod and thermally anchored to the cryostat helium reservoir. A 25 mum sapphire window separates the vacuum space from the RT sample. A positioning mechanism allows adjusting the sample-to-sensor spacing from the top of the Dewar. I have achieved a sensor-to-sample spacing of 100 mum, which could be maintained for periods of up to 4 weeks. Different SQUID sensor configurations are necessary to achieve the best combination of spatial resolution and field sensitivity for a given magnetic source. For imaging thin sections of geological samples, I used a custom-designed monolithic low-Tc niobium bare SQUID sensor, with an effective diameter of 80 mum, and achieved a field sensitivity of 1.5 pT/Hz1/2 and a magnetic moment sensitivity of 5.4 x 10-18 Am2/Hz1/2 at a sensor-to-sample spacing of 100 mum in the white noise region for frequencies above 100 Hz. Imaging action currents in cardiac tissue requires higher field sensitivity, which can only be achieved by compromising spatial resolution. I developed a monolithic low-Tc niobium multiloop SQUID sensor, with sensor sizes ranging from 250 mum to 1 mm, and achieved sensitivities of 480 - 180 fT/Hz1/2 in the white noise region for frequencies above 100 Hz, respectively. For all sensor configurations, the spatial resolution was comparable to the effective diameter and limited by the sensor-to-sample spacing. Spatial registration allowed us to compare high-resolution images of magnetic fields associated with action currents and optical recordings of transmembrane potentials to study the bidomain nature of cardiac tissue or to match petrography to magnetic field maps in thin sections of geological samples.

Interpolation of diffusion weighted imaging datasets.

PubMed

Dyrby, Tim B; Lundell, Henrik; Burke, Mark W; Reislev, Nina L; Paulson, Olaf B; Ptito, Maurice; Siebner, Hartwig R

2014-12-01

Diffusion weighted imaging (DWI) is used to study white-matter fibre organisation, orientation and structural connectivity by means of fibre reconstruction algorithms and tractography. For clinical settings, limited scan time compromises the possibilities to achieve high image resolution for finer anatomical details and signal-to-noise-ratio for reliable fibre reconstruction. We assessed the potential benefits of interpolating DWI datasets to a higher image resolution before fibre reconstruction using a diffusion tensor model. Simulations of straight and curved crossing tracts smaller than or equal to the voxel size showed that conventional higher-order interpolation methods improved the geometrical representation of white-matter tracts with reduced partial-volume-effect (PVE), except at tract boundaries. Simulations and interpolation of ex-vivo monkey brain DWI datasets revealed that conventional interpolation methods fail to disentangle fine anatomical details if PVE is too pronounced in the original data. As for validation we used ex-vivo DWI datasets acquired at various image resolutions as well as Nissl-stained sections. Increasing the image resolution by a factor of eight yielded finer geometrical resolution and more anatomical details in complex regions such as tract boundaries and cortical layers, which are normally only visualized at higher image resolutions. Similar results were found with typical clinical human DWI dataset. However, a possible bias in quantitative values imposed by the interpolation method used should be considered. The results indicate that conventional interpolation methods can be successfully applied to DWI datasets for mining anatomical details that are normally seen only at higher resolutions, which will aid in tractography and microstructural mapping of tissue compartments. Copyright © 2014. Published by Elsevier Inc.

Sub-millimetre DOI detector based on monolithic LYSO and digital SiPM for a dedicated small-animal PET system.

PubMed

Marcinkowski, Radosław; Mollet, Pieter; Van Holen, Roel; Vandenberghe, Stefaan

2016-03-07

The mouse model is widely used in a vast range of biomedical and preclinical studies. Thanks to the ability to detect and quantify biological processes at the molecular level in vivo, PET has become a well-established tool in these investigations. However, the need to visualize and quantify radiopharmaceuticals in anatomic structures of millimetre or less requires good spatial resolution and sensitivity from small-animal PET imaging systems.In previous work we have presented a proof-of-concept of a dedicated high-resolution small-animal PET scanner based on thin monolithic scintillator crystals and Digital Photon Counter photosensor. The combination of thin monolithic crystals and MLE positioning algorithm resulted in an excellent spatial resolution of 0.7 mm uniform in the entire field of view (FOV). However, the limitation of the scanner was its low sensitivity due to small thickness of the lutetium-yttrium oxyorthosilicate (LYSO) crystals (2 mm).Here we present an improved detector design for a small-animal PET system that simultaneously achieves higher sensitivity and sustains a sub-millimetre spatial resolution. The proposed detector consists of a 5 mm thick monolithic LYSO crystal optically coupled to a Digital Photon Counter. Mean nearest neighbour (MNN) positioning combined with depth of interaction (DOI) decoding was employed to achieve sub-millimetre spatial resolution. To evaluate detector performance the intrinsic spatial resolution, energy resolution and coincidence resolving time (CRT) were measured. The average intrinsic spatial resolution of the detector was 0.60 mm full-width-at-half-maximum (FWHM). A DOI resolution of 1.66 mm was achieved. The energy resolution was 23% FWHM at 511 keV and CRT of 529 ps were measured. The improved detector design overcomes the sensitivity limitation of the previous design by increasing the nominal sensitivity of the detector block and retains an excellent intrinsic spatial resolution.

High count-rate study of two TES x-ray microcalorimeters with different transition temperatures

NASA Astrophysics Data System (ADS)

Lee, Sang-Jun; Adams, Joseph S.; Bandler, Simon R.; Betancourt-Martinez, Gabriele L.; Chervenak, James A.; Eckart, Megan E.; Finkbeiner, Fred M.; Kelley, Richard L.; Kilbourne, Caroline A.; Porter, Frederick S.; Sadleir, John E.; Smith, Stephen J.; Wassell, Edward J.

2017-10-01

We have developed transition-edge sensor (TES) microcalorimeter arrays with high count-rate capability and high energy resolution to carry out x-ray imaging spectroscopy observations of various astronomical sources and the Sun. We have studied the dependence of the energy resolution and throughput (fraction of processed pulses) on the count rate for such microcalorimeters with two different transition temperatures (T c). Devices with both transition temperatures were fabricated within a single microcalorimeter array directly on top of a solid substrate where the thermal conductance of the microcalorimeter is dependent upon the thermal boundary resistance between the TES sensor and the dielectric substrate beneath. Because the thermal boundary resistance is highly temperature dependent, the two types of device with different T cs had very different thermal decay times, approximately one order of magnitude different. In our earlier report, we achieved energy resolutions of 1.6 and 2.3 eV at 6 keV from lower and higher T c devices, respectively, using a standard analysis method based on optimal filtering in the low flux limit. We have now measured the same devices at elevated x-ray fluxes ranging from 50 Hz to 1000 Hz per pixel. In the high flux limit, however, the standard optimal filtering scheme nearly breaks down because of x-ray pile-up. To achieve the highest possible energy resolution for a fixed throughput, we have developed an analysis scheme based on the so-called event grade method. Using the new analysis scheme, we achieved 5.0 eV FWHM with 96% throughput for 6 keV x-rays of 1025 Hz per pixel with the higher T c (faster) device, and 5.8 eV FWHM with 97% throughput with the lower T c (slower) device at 722 Hz.

Optimal design of an earth observation optical system with dual spectral and high resolution

NASA Astrophysics Data System (ADS)

Yan, Pei-pei; Jiang, Kai; Liu, Kai; Duan, Jing; Shan, Qiusha

2017-02-01

With the increasing demand of the high-resolution remote sensing images by military and civilians, Countries around the world are optimistic about the prospect of higher resolution remote sensing images. Moreover, design a visible/infrared integrative optic system has important value in earth observation. Because visible system can't identify camouflage and recon at night, so we should associate visible camera with infrared camera. An earth observation optical system with dual spectral and high resolution is designed. The paper mainly researches on the integrative design of visible and infrared optic system, which makes the system lighter and smaller, and achieves one satellite with two uses. The working waveband of the system covers visible, middle infrared (3-5um). Dual waveband clear imaging is achieved with dispersive RC system. The focal length of visible system is 3056mm, F/# is 10.91. And the focal length of middle infrared system is 1120mm, F/# is 4. In order to suppress the middle infrared thermal radiation and stray light, the second imaging system is achieved and the narcissus phenomenon is analyzed. The system characteristic is that the structure is simple. And the especial requirements of the Modulation Transfer Function (MTF), spot, energy concentration, and distortion etc. are all satisfied.

Determination of glucose concentrations using photonic crystal LEDs

NASA Astrophysics Data System (ADS)

Liao, Yu-Yang; Chen, Yung-Tsan; Chang, Cheng-Yu; Lan, Wen-Yi; Huang, Jian-Jang

2016-09-01

As internet of things (IOT) has become a popular topic in current consumer electronics, there is a demand for cost-effective sensors to monitor bio-signals. Traditional optical sensors employ low-dimensional gratings and high-resolution spectrometers to detect the refractive index changes of the solutions. In this work, we develop an alternative approach to correlate the concentration of molecules to the band diagrams of the photonic crystals. A relatively low-resolution spectrum analyzer can be employed, yet achieves higher sensitivity than traditional approaches.

Viking Imaging of Phobos and Deimos: An Overview of the Primary Mission

NASA Technical Reports Server (NTRS)

Duxbury, T. C.; Veverka, J.

1977-01-01

During the Viking primary mission the cameras on the two orbiters acquired about 50 pictures of the two Martian moons. The Viking images of the satellites have a higher surface resolution than those obtained by Mariner 9. The typical surface resolution achieved was 100-200 m, although detail as small as 40 m was imaged on Phobos during a particularly close passage. Attention is given to color sequences obtained for each satellite, aspects of phase angle coverage, and pictures for ephemeris improvement.

High-speed and high-resolution quantitative phase imaging with digital-micromirror device-based illumination (Conference Presentation)

NASA Astrophysics Data System (ADS)

Zhou, Renjie; Jin, Di; Yaqoob, Zahid; So, Peter T. C.

2017-02-01

Due to the large number of available mirrors, the patterning speed, low-cost, and compactness, digital-micromirror devices (DMDs) have been extensively used in biomedical imaging system. Recently, DMDs have been brought to the quantitative phase microscopy (QPM) field to achieve synthetic-aperture imaging and tomographic imaging. Last year, our group demonstrated using DMD for QPM, where the phase-retrieval is based on a recently developed Fourier ptychography algorithm. In our previous system, the illumination angle was varied through coding the aperture plane of the illumination system, which has a low efficiency on utilizing the laser power. In our new DMD-based QPM system, we use the Lee-holograms, which is conjugated to the sample plane, to change the illumination angles for much higher power efficiency. Multiple-angle illumination can also be achieved with this method. With this versatile system, we can achieve FPM-based high-resolution phase imaging with 250 nm lateral resolution using the Rayleigh criteria. Due to the use of a powerful laser, the imaging speed would only be limited by the camera acquisition speed. With a fast camera, we expect to achieve close to 100 fps phase imaging speed that has not been achieved in current FPM imaging systems. By adding reference beam, we also expect to achieve synthetic-aperture imaging while directly measuring the phase of the sample fields. This would reduce the phase-retrieval processing time to allow for real-time imaging applications in the future.

Detector motion method to increase spatial resolution in photon-counting detectors

NASA Astrophysics Data System (ADS)

Lee, Daehee; Park, Kyeongjin; Lim, Kyung Taek; Cho, Gyuseong

2017-03-01

Medical imaging requires high spatial resolution of an image to identify fine lesions. Photon-counting detectors in medical imaging have recently been rapidly replacing energy-integrating detectors due to the former`s high spatial resolution, high efficiency and low noise. Spatial resolution in a photon counting image is determined by the pixel size. Therefore, the smaller the pixel size, the higher the spatial resolution that can be obtained in an image. However, detector redesigning is required to reduce pixel size, and an expensive fine process is required to integrate a signal processing unit with reduced pixel size. Furthermore, as the pixel size decreases, charge sharing severely deteriorates spatial resolution. To increase spatial resolution, we propose a detector motion method using a large pixel detector that is less affected by charge sharing. To verify the proposed method, we utilized a UNO-XRI photon-counting detector (1-mm CdTe, Timepix chip) at the maximum X-ray tube voltage of 80 kVp. A similar spatial resolution of a 55- μm-pixel image was achieved by application of the proposed method to a 110- μm-pixel detector with a higher signal-to-noise ratio. The proposed method could be a way to increase spatial resolution without a pixel redesign when pixels severely suffer from charge sharing as pixel size is reduced.

Operational characteristics of Wedge and Strip image readout systems

NASA Technical Reports Server (NTRS)

Siegmund, O. H. W.; Lampton, M.; Bixler, J.; Bowyer, S.; Malina, R. F.

1986-01-01

Application of the Wedge and Strip readout system in microchannel plate detectors for the Extreme Ultraviolet Explorer and FAUST space astronomy programs is discussed. Anode designs with high resolution (greater than 600 x 600 pixels) in imaging and spectroscopy applications have been developed. Extension of these designs to larger formats (100 mm) with higher resolution (3000 x 3000 pixels) are considered. It is shown that the resolution and imaging are highly stable, and that the flat field performance is essentially limited by photon statistics. Very high speed event response has also been achieved with output pulses having durations of less than 10 nanoseconds.

A commercialized photoacoustic microscopy system with switchable optical and acoustic resolutions

NASA Astrophysics Data System (ADS)

Pu, Yang; Bi, Renzhe; Olivo, Malini; Zhao, Xiaojie

2018-02-01

A focused-scanning photoacoustic microscopy (PAM) is available to help advancing life science research in neuroscience, cell biology, and in vivo imaging. At this early stage, the only one manufacturer of PAM systems, MicroPhotoAcoustics (MPA; Ronkonkoma, NY), MPA has developed a commercial PAM system with switchable optical and acoustic resolution (OR- and AR-PAM), using multiple patents licensed from the lab of Lihong Wang, who pioneered photoacoustics. The system includes different excitation sources. Two kilohertz-tunable, Q-switched, Diode Pumped Solid-State (DPSS) lasers offering a up to 30kHz pulse repetition rate and 9 ns pulse duration with 532 and 559 nm to achieve functional photoacoustic tomography for sO2 (oxygen saturation of hemoglobin) imaging in OR-PAM. A Ti:sapphire laser from 700 to 900 nm to achieve deep-tissue imaging. OR-PAM provides up to 1 mm penetration depth and 5 μm lateral resolution. while AR-PAM offers up to 3 mm imaging depth and 45 μm lateral resolution. The scanning step sizes for OR- and AR-PAM are 0.625 and 6.25 μm, respectively. Researchers have used the system for a range of applications, including preclinical neural imaging; imaging of cell nuclei in intestine, ear, and leg; and preclinical human imaging of finger cuticle. With the continuation of new technological advancements and discoveries, MPA plans to further advance PAM to achieve faster imaging speed, higher spatial resolution at deeper tissue layer, and address a broader range of biomedical applications.

Evaluation and Comparison of High-Resolution (HR) and High-Light (HL) Phosphors in the Micro-Angiographic Fluoroscope (MAF) using Generalized Linear Systems Analyses (GMTF, GDQE) that include the Effect of Scatter, Magnification and Detector Characteristics.

PubMed

Gupta, Sandesh K; Jain, Amit; Bednarek, Daniel R; Rudin, Stephen

2011-01-01

In this study, we evaluated the imaging characteristics of the high-resolution, high-sensitivity micro-angiographic fluoroscope (MAF) with 35-micron pixel-pitch when used with different commercially-available 300 micron thick phosphors: the high resolution (HR) and high light (HL) from Hamamatsu. The purpose of this evaluation was to see if the HL phosphor with its higher screen efficiency could be replaced with the HR phosphor to achieve improved resolution without an increase in noise resulting from the HR's decreased light-photon yield. We designated the detectors MAF-HR and MAF-HL and compared them with a standard flat panel detector (FPD) (194 micron pixel pitch and 600 micron thick CsI(Tl)). For this comparison, we used the generalized linear-system metrics of GMTF, GNNPS and GDQE which are more realistic measures of total system performance since they include the effect of scattered radiation, focal spot distribution, and geometric un-sharpness. Magnifications (1.05-1.15) and scatter fractions (0.28 and 0.33) characteristic of a standard head phantom were used. The MAF-HR performed significantly better than the MAF-HL at high spatial frequencies. The ratio of GMTF and GDQE of the MAF-HR compared to the MAF-HL at 3(6) cycles/mm was 1.45(2.42) and 1.23(2.89), respectively. Despite significant degradation by inclusion of scatter and object magnification, both MAF-HR and MAF-HL provide superior performance over the FPD at higher spatial frequencies with similar performance up to the FPD's Nyquist frequency of 2.5 cycles/mm. Both substantially higher resolution and improved GDQE can be achieved with the MAF using the HR phosphor instead of the HL phosphor.

Determining Extension's Role in Controversial Issues: Content, Process, Neither, or Both?

ERIC Educational Resources Information Center

Goerlich, Dan; Walker, Martha A.

2015-01-01

Controversial issues offer Extension faculty opportunities to facilitate community dialogue and apply conflict resolution strategies to help communities achieve higher ground. Handled appropriately, the long-term benefits to the community, the Extension organization, and the faculty member of facilitating public issues outweigh the costs. This…

High-resolution quantization based on soliton self-frequency shift and spectral compression in a bi-directional comb-fiber architecture

NASA Astrophysics Data System (ADS)

Zhang, Xuyan; Zhang, Zhiyao; Wang, Shubing; Liang, Dong; Li, Heping; Liu, Yong

2018-03-01

We propose and demonstrate an approach that can achieve high-resolution quantization by employing soliton self-frequency shift and spectral compression. Our approach is based on a bi-directional comb-fiber architecture which is composed of a Sagnac-loop-based mirror and a comb-like combination of N sections of interleaved single-mode fibers and high nonlinear fibers. The Sagnac-loop-based mirror placed at the terminal of a bus line reflects the optical pulses back to the bus line to achieve additional N-stage spectral compression, thus single-stage soliton self-frequency shift (SSFS) and (2 N - 1)-stage spectral compression are realized in the bi-directional scheme. The fiber length in the architecture is numerically optimized, and the proposed quantization scheme is evaluated by both simulation and experiment in the case of N = 2. In the experiment, a quantization resolution of 6.2 bits is obtained, which is 1.2-bit higher than that of its uni-directional counterpart.

Design and performance evaluation of a new high energy parallel hole collimator for radioiodine planar imaging by gamma cameras: Monte Carlo simulation study.

PubMed

Moslemi, Vahid; Ashoor, Mansour

2017-05-01

In addition to the trade-off between resolution and sensitivity which is a common problem among all types of parallel hole collimators (PCs), obtained images by high energy PCs (HEPCs) suffer from hole-pattern artifact (HPA) due to further septa thickness. In this study, a new design on the collimator has been proposed to improve the trade-off between resolution and sensitivity and to eliminate the HPA. A novel PC, namely high energy extended PC (HEEPC), is proposed and is compared to HEPCs. In the new PC, trapezoidal denticles were added upon the septa in the detector side. The performance of the HEEPCs were evaluated and compared to that of HEPCs using a Monte Carlo-N-particle version5 (MCNP5) simulation. The point spread functions (PSF) of HEPCs and HEEPCs were obtained as well as the various parameters such as resolution, sensitivity, scattering, and penetration ratios, and the HPA of the collimators was assessed. Furthermore, a Picker phantom study was performed to examine the effects of the collimators on the quality of planar images. It was found that the HEEPC D with an identical resolution to that of HEPC C increased sensitivity by 34.7%, and it improved the trade-off between resolution and sensitivity as well as to eliminate the HPA. In the picker phantom study, the HEEPC D indicated the hot and cold lesions with the higher contrast, lower noise, and higher contrast to noise ratio (CNR). Since the HEEPCs modify the shaping of PSFs, they are able to improve the trade-off between the resolution and sensitivity; consequently, planar images can be achieved with higher contrast resolutions. Furthermore, because the HEEPC S reduce the HPA and produce images with a higher CNR, compared to HEPCs, the obtained images by HEEPCs have a higher quality, which can help physicians to provide better diagnosis.

High Resolution Trichromatic Road Surface Scanning with a Line Scan Camera and Light Emitting Diode Lighting for Road-Kill Detection.

PubMed

Lopes, Gil; Ribeiro, A Fernando; Sillero, Neftalí; Gonçalves-Seco, Luís; Silva, Cristiano; Franch, Marc; Trigueiros, Paulo

2016-04-19

This paper presents a road surface scanning system that operates with a trichromatic line scan camera with light emitting diode (LED) lighting achieving road surface resolution under a millimeter. It was part of a project named Roadkills-Intelligent systems for surveying mortality of amphibians in Portuguese roads, sponsored by the Portuguese Science and Technology Foundation. A trailer was developed in order to accommodate the complete system with standalone power generation, computer image capture and recording, controlled lighting to operate day or night without disturbance, incremental encoder with 5000 pulses per revolution attached to one of the trailer wheels, under a meter Global Positioning System (GPS) localization, easy to utilize with any vehicle with a trailer towing system and focused on a complete low cost solution. The paper describes the system architecture of the developed prototype, its calibration procedure, the performed experimentation and some obtained results, along with a discussion and comparison with existing systems. Sustained operating trailer speeds of up to 30 km/h are achievable without loss of quality at 4096 pixels' image width (1 m width of road surface) with 250 µm/pixel resolution. Higher scanning speeds can be achieved by lowering the image resolution (120 km/h with 1 mm/pixel). Computer vision algorithms are under development to operate on the captured images in order to automatically detect road-kills of amphibians.

High Resolution Trichromatic Road Surface Scanning with a Line Scan Camera and Light Emitting Diode Lighting for Road-Kill Detection

PubMed Central

Lopes, Gil; Ribeiro, A. Fernando; Sillero, Neftalí; Gonçalves-Seco, Luís; Silva, Cristiano; Franch, Marc; Trigueiros, Paulo

2016-01-01

This paper presents a road surface scanning system that operates with a trichromatic line scan camera with light emitting diode (LED) lighting achieving road surface resolution under a millimeter. It was part of a project named Roadkills—Intelligent systems for surveying mortality of amphibians in Portuguese roads, sponsored by the Portuguese Science and Technology Foundation. A trailer was developed in order to accommodate the complete system with standalone power generation, computer image capture and recording, controlled lighting to operate day or night without disturbance, incremental encoder with 5000 pulses per revolution attached to one of the trailer wheels, under a meter Global Positioning System (GPS) localization, easy to utilize with any vehicle with a trailer towing system and focused on a complete low cost solution. The paper describes the system architecture of the developed prototype, its calibration procedure, the performed experimentation and some obtained results, along with a discussion and comparison with existing systems. Sustained operating trailer speeds of up to 30 km/h are achievable without loss of quality at 4096 pixels’ image width (1 m width of road surface) with 250 µm/pixel resolution. Higher scanning speeds can be achieved by lowering the image resolution (120 km/h with 1 mm/pixel). Computer vision algorithms are under development to operate on the captured images in order to automatically detect road-kills of amphibians. PMID:27104535

High-resolution room-temperature sample scanning superconducting quantum interference device microscope configurable for geological and biomagnetic applications

NASA Astrophysics Data System (ADS)

Fong, L. E.; Holzer, J. R.; McBride, K. K.; Lima, E. A.; Baudenbacher, F.; Radparvar, M.

2005-05-01

We have developed a scanning superconducting quantum interference device (SQUID) microscope system with interchangeable sensor configurations for imaging magnetic fields of room-temperature (RT) samples with submillimeter resolution. The low-critical-temperature (Tc) niobium-based monolithic SQUID sensors are mounted on the tip of a sapphire and thermally anchored to the helium reservoir. A 25μm sapphire window separates the vacuum space from the RT sample. A positioning mechanism allows us to adjust the sample-to-sensor spacing from the top of the Dewar. We achieved a sensor-to-sample spacing of 100μm, which could be maintained for periods of up to four weeks. Different SQUID sensor designs are necessary to achieve the best combination of spatial resolution and field sensitivity for a given source configuration. For imaging thin sections of geological samples, we used a custom-designed monolithic low-Tc niobium bare SQUID sensor, with an effective diameter of 80μm, and achieved a field sensitivity of 1.5pT/Hz1/2 and a magnetic moment sensitivity of 5.4×10-18Am2/Hz1/2 at a sensor-to-sample spacing of 100μm in the white noise region for frequencies above 100Hz. Imaging action currents in cardiac tissue requires a higher field sensitivity, which can only be achieved by compromising spatial resolution. We developed a monolithic low-Tc niobium multiloop SQUID sensor, with sensor sizes ranging from 250μm to 1mm, and achieved sensitivities of 480-180fT /Hz1/2 in the white noise region for frequencies above 100Hz, respectively. For all sensor configurations, the spatial resolution was comparable to the effective diameter and limited by the sensor-to-sample spacing. Spatial registration allowed us to compare high-resolution images of magnetic fields associated with action currents and optical recordings of transmembrane potentials to study the bidomain nature of cardiac tissue or to match petrography to magnetic field maps in thin sections of geological samples.

A High-Resolution Demodulation Algorithm for FBG-FP Static-Strain Sensors Based on the Hilbert Transform and Cross Third-Order Cumulant

PubMed Central

Huang, Wenzhu; Zhen, Tengkun; Zhang, Wentao; Zhang, Fusheng; Li, Fang

2015-01-01

Static strain can be detected by measuring a cross-correlation of reflection spectra from two fiber Bragg gratings (FBGs). However, the static-strain measurement resolution is limited by the dominant Gaussian noise source when using this traditional method. This paper presents a novel static-strain demodulation algorithm for FBG-based Fabry-Perot interferometers (FBG-FPs). The Hilbert transform is proposed for changing the Gaussian distribution of the two FBG-FPs’ reflection spectra, and a cross third-order cumulant is used to use the results of the Hilbert transform and get a group of noise-vanished signals which can be used to accurately calculate the wavelength difference of the two FBG-FPs. The benefit by these processes is that Gaussian noise in the spectra can be suppressed completely in theory and a higher resolution can be reached. In order to verify the precision and flexibility of this algorithm, a detailed theory model and a simulation analysis are given, and an experiment is implemented. As a result, a static-strain resolution of 0.9 nε under laboratory environment condition is achieved, showing a higher resolution than the traditional cross-correlation method. PMID:25923938

A High-Resolution Demodulation Algorithm for FBG-FP Static-Strain Sensors Based on the Hilbert Transform and Cross Third-Order Cumulant.

PubMed

Huang, Wenzhu; Zhen, Tengkun; Zhang, Wentao; Zhang, Fusheng; Li, Fang

2015-04-27

Static strain can be detected by measuring a cross-correlation of reflection spectra from two fiber Bragg gratings (FBGs). However, the static-strain measurement resolution is limited by the dominant Gaussian noise source when using this traditional method. This paper presents a novel static-strain demodulation algorithm for FBG-based Fabry-Perot interferometers (FBG-FPs). The Hilbert transform is proposed for changing the Gaussian distribution of the two FBG-FPs' reflection spectra, and a cross third-order cumulant is used to use the results of the Hilbert transform and get a group of noise-vanished signals which can be used to accurately calculate the wavelength difference of the two FBG-FPs. The benefit by these processes is that Gaussian noise in the spectra can be suppressed completely in theory and a higher resolution can be reached. In order to verify the precision and flexibility of this algorithm, a detailed theory model and a simulation analysis are given, and an experiment is implemented. As a result, a static-strain resolution of 0.9 nε under laboratory environment condition is achieved, showing a higher resolution than the traditional cross-correlation method.

Adaptive optics for peripheral vision

NASA Astrophysics Data System (ADS)

Rosén, R.; Lundström, L.; Unsbo, P.

2012-07-01

Understanding peripheral optical errors and their impact on vision is important for various applications, e.g. research on myopia development and optical correction of patients with central visual field loss. In this study, we investigated whether correction of higher order aberrations with adaptive optics (AO) improve resolution beyond what is achieved with best peripheral refractive correction. A laboratory AO system was constructed for correcting peripheral aberrations. The peripheral low contrast grating resolution acuity in the 20° nasal visual field of the right eye was evaluated for 12 subjects using three types of correction: refractive correction of sphere and cylinder, static closed loop AO correction and continuous closed loop AO correction. Running AO in continuous closed loop improved acuity compared to refractive correction for most subjects (maximum benefit 0.15 logMAR). The visual improvement from aberration correction was highly correlated with the subject's initial amount of higher order aberrations (p = 0.001, R 2 = 0.72). There was, however, no acuity improvement from static AO correction. In conclusion, correction of peripheral higher order aberrations can improve low contrast resolution, provided refractive errors are corrected and the system runs in continuous closed loop.

High spatial resolution diffusion weighted imaging on clinical 3 T MRI scanners using multislab spiral acquisitions

PubMed Central

Holtrop, Joseph L.; Sutton, Bradley P.

2016-01-01

Abstract. A diffusion weighted imaging (DWI) approach that is signal-to-noise ratio (SNR) efficient and can be applied to achieve sub-mm resolutions on clinical 3 T systems was developed. The sequence combined a multislab, multishot pulsed gradient spin echo diffusion scheme with spiral readouts for imaging data and navigators. Long data readouts were used to keep the number of shots, and hence total imaging time, for the three-dimensional acquisition short. Image quality was maintained by incorporating a field-inhomogeneity-corrected image reconstruction to remove distortions associated with long data readouts. Additionally, multiple shots were required for the high-resolution images, necessitating motion induced phase correction through the use of efficiently integrated navigator data. The proposed approach is compared with two-dimensional (2-D) acquisitions that use either a spiral or a typical echo-planar imaging (EPI) acquisition to demonstrate the improved SNR efficiency. The proposed technique provided 71% higher SNR efficiency than the standard 2-D EPI approach. The adaptability of the technique to achieve high spatial resolutions is demonstrated by acquiring diffusion tensor imaging data sets with isotropic resolutions of 1.25 and 0.8 mm. The proposed approach allows for SNR-efficient sub-mm acquisitions of DWI data on clinical 3 T systems. PMID:27088107

Object-Based Paddy Rice Mapping Using HJ-1A/B Data and Temporal Features Extracted from Time Series MODIS NDVI Data

PubMed Central

Singha, Mrinal; Wu, Bingfang; Zhang, Miao

2016-01-01

Accurate and timely mapping of paddy rice is vital for food security and environmental sustainability. This study evaluates the utility of temporal features extracted from coarse resolution data for object-based paddy rice classification of fine resolution data. The coarse resolution vegetation index data is first fused with the fine resolution data to generate the time series fine resolution data. Temporal features are extracted from the fused data and added with the multi-spectral data to improve the classification accuracy. Temporal features provided the crop growth information, while multi-spectral data provided the pattern variation of paddy rice. The achieved overall classification accuracy and kappa coefficient were 84.37% and 0.68, respectively. The results indicate that the use of temporal features improved the overall classification accuracy of a single-date multi-spectral image by 18.75% from 65.62% to 84.37%. The minimum sensitivity (MS) of the paddy rice classification has also been improved. The comparison showed that the mapped paddy area was analogous to the agricultural statistics at the district level. This work also highlighted the importance of feature selection to achieve higher classification accuracies. These results demonstrate the potential of the combined use of temporal and spectral features for accurate paddy rice classification. PMID:28025525

Object-Based Paddy Rice Mapping Using HJ-1A/B Data and Temporal Features Extracted from Time Series MODIS NDVI Data.

PubMed

Singha, Mrinal; Wu, Bingfang; Zhang, Miao

2016-12-22

Accurate and timely mapping of paddy rice is vital for food security and environmental sustainability. This study evaluates the utility of temporal features extracted from coarse resolution data for object-based paddy rice classification of fine resolution data. The coarse resolution vegetation index data is first fused with the fine resolution data to generate the time series fine resolution data. Temporal features are extracted from the fused data and added with the multi-spectral data to improve the classification accuracy. Temporal features provided the crop growth information, while multi-spectral data provided the pattern variation of paddy rice. The achieved overall classification accuracy and kappa coefficient were 84.37% and 0.68, respectively. The results indicate that the use of temporal features improved the overall classification accuracy of a single-date multi-spectral image by 18.75% from 65.62% to 84.37%. The minimum sensitivity (MS) of the paddy rice classification has also been improved. The comparison showed that the mapped paddy area was analogous to the agricultural statistics at the district level. This work also highlighted the importance of feature selection to achieve higher classification accuracies. These results demonstrate the potential of the combined use of temporal and spectral features for accurate paddy rice classification.

High spatial resolution upgrade of the electron cyclotron emission radiometer for the DIII-D tokamak.

PubMed

Truong, D D; Austin, M E

2014-11-01

The 40-channel DIII-D electron cyclotron emission (ECE) radiometer provides measurements of Te(r,t) at the tokamak midplane from optically thick, second harmonic X-mode emission over a frequency range of 83-130 GHz. The frequency spacing of the radiometer's channels results in a spatial resolution of ∼1-3 cm, depending on local magnetic field and electron temperature. A new high resolution subsystem has been added to the DIII-D ECE radiometer to make sub-centimeter (0.6-0.8 cm) resolution Te measurements. The high resolution subsystem branches off from the regular channels' IF bands and consists of a microwave switch to toggle between IF bands, a switched filter bank for frequency selectivity, an adjustable local oscillator and mixer for further frequency down-conversion, and a set of eight microwave filters in the 2-4 GHz range. Higher spatial resolution is achieved through the use of a narrower (200 MHz) filter bandwidth and closer spacing between the filters' center frequencies (250 MHz). This configuration allows for full coverage of the 83-130 GHz frequency range in 2 GHz bands. Depending on the local magnetic field, this translates into a "zoomed-in" analysis of a ∼2-4 cm radial region. Expected uses of these channels include mapping the spatial dependence of Alfven eigenmodes, geodesic acoustic modes, and externally applied magnetic perturbations. Initial Te measurements, which demonstrate that the desired resolution is achieved, are presented.

Fundamental limits of reconstruction-based superresolution algorithms under local translation.

PubMed

Lin, Zhouchen; Shum, Heung-Yeung

2004-01-01

Superresolution is a technique that can produce images of a higher resolution than that of the originally captured ones. Nevertheless, improvement in resolution using such a technique is very limited in practice. This makes it significant to study the problem: "Do fundamental limits exist for superresolution?" In this paper, we focus on a major class of superresolution algorithms, called the reconstruction-based algorithms, which compute high-resolution images by simulating the image formation process. Assuming local translation among low-resolution images, this paper is the first attempt to determine the explicit limits of reconstruction-based algorithms, under both real and synthetic conditions. Based on the perturbation theory of linear systems, we obtain the superresolution limits from the conditioning analysis of the coefficient matrix. Moreover, we determine the number of low-resolution images that are sufficient to achieve the limit. Both real and synthetic experiments are carried out to verify our analysis.

High-Resolution Underwater Mapping Using Side-Scan Sonar

PubMed Central

2016-01-01

The goal of this study is to generate high-resolution sea floor maps using a Side-Scan Sonar(SSS). This is achieved by explicitly taking into account the SSS operation as follows. First, the raw sensor data is corrected by means of a physics-based SSS model. Second, the data is projected to the sea-floor. The errors involved in this projection are thoroughfully analysed. Third, a probabilistic SSS model is defined and used to estimate the probability of each sea-floor region to be observed. This probabilistic information is then used to weight the contribution of each SSS measurement to the map. Because of these models, arbitrary map resolutions can be achieved, even beyond the sensor resolution. Finally, a geometric map building method is presented and combined with the probabilistic approach. The resulting map is composed of two layers. The echo intensity layer holds the most likely echo intensities at each point in the sea-floor. The probabilistic layer contains information about how confident can the user or the higher control layers be about the echo intensity layer data. Experimental results have been conducted in a large subsea region. PMID:26821379

Computation of Surface Laplacian for tri-polar ring electrodes on high-density realistic geometry head model.

PubMed

Junwei Ma; Han Yuan; Sunderam, Sridhar; Besio, Walter; Lei Ding

2017-07-01

Neural activity inside the human brain generate electrical signals that can be detected on the scalp. Electroencephalograph (EEG) is one of the most widely utilized techniques helping physicians and researchers to diagnose and understand various brain diseases. Due to its nature, EEG signals have very high temporal resolution but poor spatial resolution. To achieve higher spatial resolution, a novel tri-polar concentric ring electrode (TCRE) has been developed to directly measure Surface Laplacian (SL). The objective of the present study is to accurately calculate SL for TCRE based on a realistic geometry head model. A locally dense mesh was proposed to represent the head surface, where the local dense parts were to match the small structural components in TCRE. Other areas without dense mesh were used for the purpose of reducing computational load. We conducted computer simulations to evaluate the performance of the proposed mesh and evaluated possible numerical errors as compared with a low-density model. Finally, with achieved accuracy, we presented the computed forward lead field of SL for TCRE for the first time in a realistic geometry head model and demonstrated that it has better spatial resolution than computed SL from classic EEG recordings.

Comparing the imaging performance of computed super resolution and magnification tomosynthesis

NASA Astrophysics Data System (ADS)

Maidment, Tristan D.; Vent, Trevor L.; Ferris, William S.; Wurtele, David E.; Acciavatti, Raymond J.; Maidment, Andrew D. A.

2017-03-01

Computed super-resolution (SR) is a method of reconstructing images with pixels that are smaller than the detector element size; superior spatial resolution is achieved through the elimination of aliasing and alteration of the sampling function imposed by the reconstructed pixel aperture. By comparison, magnification mammography is a method of projection imaging that uses geometric magnification to increase spatial resolution. This study explores the development and application of magnification digital breast tomosynthesis (MDBT). Four different acquisition geometries are compared in terms of various image metrics. High-contrast spatial resolution was measured in various axes using a lead star pattern. A modified Defrise phantom was used to determine the low-frequency spatial resolution. An anthropomorphic phantom was used to simulate clinical imaging. Each experiment was conducted at three different magnifications: contact (1.04x), MAG1 (1.3x), and MAG2 (1.6x). All images were taken on our next generation tomosynthesis system, an in-house solution designed to optimize SR. It is demonstrated that both computed SR and MDBT (MAG1 and MAG2) provide improved spatial resolution over non-SR contact imaging. To achieve the highest resolution, SR and MDBT should be combined. However, MDBT is adversely affected by patient motion at higher magnifications. In addition, MDBT requires more radiation dose and delays diagnosis, since MDBT would be conducted upon recall. By comparison, SR can be conducted with the original screening data. In conclusion, this study demonstrates that computed SR and MDBT are both viable methods of imaging the breast.

Coherence and diffraction limited resolution in microscopic OCT by a unified approach for the correction of dispersion and aberrations

NASA Astrophysics Data System (ADS)

Schulz-Hildebrandt, H.; Münter, Michael; Ahrens, M.; Spahr, H.; Hillmann, D.; König, P.; Hüttmann, G.

2018-03-01

Optical coherence tomography (OCT) images scattering tissues with 5 to 15 μm resolution. This is usually not sufficient for a distinction of cellular and subcellular structures. Increasing axial and lateral resolution and compensation of artifacts caused by dispersion and aberrations is required to achieve cellular and subcellular resolution. This includes defocus which limit the usable depth of field at high lateral resolution. OCT gives access the phase of the scattered light and hence correction of dispersion and aberrations is possible by numerical algorithms. Here we present a unified dispersion/aberration correction which is based on a polynomial parameterization of the phase error and an optimization of the image quality using Shannon's entropy. For validation, a supercontinuum light sources and a costume-made spectrometer with 400 nm bandwidth were combined with a high NA microscope objective in a setup for tissue and small animal imaging. Using this setup and computation corrections, volumetric imaging at 1.5 μm resolution is possible. Cellular and near cellular resolution is demonstrated in porcine cornea and the drosophila larva, when computational correction of dispersion and aberrations is used. Due to the excellent correction of the used microscope objective, defocus was the main contribution to the aberrations. In addition, higher aberrations caused by the sample itself were successfully corrected. Dispersion and aberrations are closely related artifacts in microscopic OCT imaging. Hence they can be corrected in the same way by optimization of the image quality. This way microscopic resolution is easily achieved in OCT imaging of static biological tissues.

High Count-Rate Study of Two TES X-Ray Microcalorimeters With Different Transition Temperatures

NASA Technical Reports Server (NTRS)

Lee, Sang-Jun; Adams, Joseph S.; Bandler, Simon R.; Betancourt-Martinez, Gabriele L.; Chervenak, James A.; Eckart, Megan E.; Finkbeiner, Fred M.; Kelley, Richard L.; Kilbourne, Caroline A.; Porter, Frederick S.;

Temporal resolution improvement using PICCS in MDCT cardiac imaging

PubMed Central

Chen, Guang-Hong; Tang, Jie; Hsieh, Jiang

2009-01-01

The current paradigm for temporal resolution improvement is to add more source-detector units and∕or increase the gantry rotation speed. The purpose of this article is to present an innovative alternative method to potentially improve temporal resolution by approximately a factor of 2 for all MDCT scanners without requiring hardware modification. The central enabling technology is a most recently developed image reconstruction method: Prior image constrained compressed sensing (PICCS). Using the method, cardiac CT images can be accurately reconstructed using the projection data acquired in an angular range of about 120°, which is roughly 50% of the standard short-scan angular range (∼240° for an MDCT scanner). As a result, the temporal resolution of MDCT cardiac imaging can be universally improved by approximately a factor of 2. In order to validate the proposed method, two in vivo animal experiments were conducted using a state-of-the-art 64-slice CT scanner (GE Healthcare, Waukesha, WI) at different gantry rotation times and different heart rates. One animal was scanned at heart rate of 83 beats per minute (bpm) using 400 ms gantry rotation time and the second animal was scanned at 94 bpm using 350 ms gantry rotation time, respectively. Cardiac coronary CT imaging can be successfully performed at high heart rates using a single-source MDCT scanner and projection data from a single heart beat with gantry rotation times of 400 and 350 ms. Using the proposed PICCS method, the temporal resolution of cardiac CT imaging can be effectively improved by approximately a factor of 2 without modifying any scanner hardware. This potentially provides a new method for single-source MDCT scanners to achieve reliable coronary CT imaging for patients at higher heart rates than the current heart rate limit of 70 bpm without using the well-known multisegment FBP reconstruction algorithm. This method also enables dual-source MDCT scanner to achieve higher temporal resolution without further hardware modifications. PMID:19610302

Temporal resolution improvement using PICCS in MDCT cardiac imaging.

PubMed

Chen, Guang-Hong; Tang, Jie; Hsieh, Jiang

2009-06-01

The current paradigm for temporal resolution improvement is to add more source-detector units and/or increase the gantry rotation speed. The purpose of this article is to present an innovative alternative method to potentially improve temporal resolution by approximately a factor of 2 for all MDCT scanners without requiring hardware modification. The central enabling technology is a most recently developed image reconstruction method: Prior image constrained compressed sensing (PICCS). Using the method, cardiac CT images can be accurately reconstructed using the projection data acquired in an angular range of about 120 degrees, which is roughly 50% of the standard short-scan angular range (approximately 240 degrees for an MDCT scanner). As a result, the temporal resolution of MDCT cardiac imaging can be universally improved by approximately a factor of 2. In order to validate the proposed method, two in vivo animal experiments were conducted using a state-of-the-art 64-slice CT scanner (GE Healthcare, Waukesha, WI) at different gantry rotation times and different heart rates. One animal was scanned at heart rate of 83 beats per minute (bpm) using 400 ms gantry rotation time and the second animal was scanned at 94 bpm using 350 ms gantry rotation time, respectively. Cardiac coronary CT imaging can be successfully performed at high heart rates using a single-source MDCT scanner and projection data from a single heart beat with gantry rotation times of 400 and 350 ms. Using the proposed PICCS method, the temporal resolution of cardiac CT imaging can be effectively improved by approximately a factor of 2 without modifying any scanner hardware. This potentially provides a new method for single-source MDCT scanners to achieve reliable coronary CT imaging for patients at higher heart rates than the current heart rate limit of 70 bpm without using the well-known multisegment FBP reconstruction algorithm. This method also enables dual-source MDCT scanner to achieve higher temporal resolution without further hardware modifications.

Evaluation of computational endomicroscopy architectures for minimally-invasive optical biopsy

NASA Astrophysics Data System (ADS)

Dumas, John P.; Lodhi, Muhammad A.; Bajwa, Waheed U.; Pierce, Mark C.

2017-02-01

We are investigating compressive sensing architectures for applications in endomicroscopy, where the narrow diameter probes required for tissue access can limit the achievable spatial resolution. We hypothesize that the compressive sensing framework can be used to overcome the fundamental pixel number limitation in fiber-bundle based endomicroscopy by reconstructing images with more resolvable points than fibers in the bundle. An experimental test platform was assembled to evaluate and compare two candidate architectures, based on introducing a coded amplitude mask at either a conjugate image or Fourier plane within the optical system. The benchtop platform consists of a common illumination and object path followed by separate imaging arms for each compressive architecture. The imaging arms contain a digital micromirror device (DMD) as a reprogrammable mask, with a CCD camera for image acquisition. One arm has the DMD positioned at a conjugate image plane ("IP arm"), while the other arm has the DMD positioned at a Fourier plane ("FP arm"). Lenses were selected and positioned within each arm to achieve an element-to-pixel ratio of 16 (230,400 mask elements mapped onto 14,400 camera pixels). We discuss our mathematical model for each system arm and outline the importance of accounting for system non-idealities. Reconstruction of a 1951 USAF resolution target using optimization-based compressive sensing algorithms produced images with higher spatial resolution than bicubic interpolation for both system arms when system non-idealities are included in the model. Furthermore, images generated with image plane coding appear to exhibit higher spatial resolution, but more noise, than images acquired through Fourier plane coding.

Optical Analysis of an Ultra-High resolution Two-Mirror Soft X-Ray Microscope

NASA Technical Reports Server (NTRS)

Shealy, David L.; Wang, Cheng; Hoover, Richard B.

1994-01-01

This work has summarized for a Schwarzschild microscope some relationships between numerical aperture (NA), magnification, diameter of the primary mirror, radius of curvature of the secondary mirror, and the total length of the microscope. To achieve resolutions better than a spherical Schwarzschild microscope of 3.3 Lambda for a perfectly aligned and fabricated system. it is necessary to use aspherical surfaces to control higher-order aberrations. For an NA of 0.35, the aspherical Head microscope provides diffraction limited resolution of 1.4 Lambda where the aspherical surfaces differ from the best fit spherical surface by approximately 1 micrometer. However, the angle of incidence varies significantly over the primary and the secondary mirrors, which will require graded multilayer coatings to operate near peak reflectivities. For higher numerical apertures, the variation of the angle of incidence over the secondary mirror surface becomes a serious problem which must be solved before multilayer coatings can be used for this application. Tolerance analysis of the spherical Schwarzschild microscope has shown that water window operations will require 2-3 times tighter tolerances to achieve a similar performance for operations with 130 A radiation. Surface contour errors have been shown to have a significant impact on the MTF and must be controlled to a peak-to-valley variation of 50-100 A and a frequency of 8 periods over the surface of a mirror.

High angular resolution at LBT

NASA Astrophysics Data System (ADS)

Conrad, A.; Arcidiacono, C.; Bertero, M.; Boccacci, P.; Davies, A. G.; Defrere, D.; de Kleer, K.; De Pater, I.; Hinz, P.; Hofmann, K. H.; La Camera, A.; Leisenring, J.; Kürster, M.; Rathbun, J. A.; Schertl, D.; Skemer, A.; Skrutskie, M.; Spencer, J. R.; Veillet, C.; Weigelt, G.; Woodward, C. E.

2015-12-01

High angular resolution from ground-based observatories stands as a key technology for advancing planetary science. In the window between the angular resolution achievable with 8-10 meter class telescopes, and the 23-to-40 meter giants of the future, LBT provides a glimpse of what the next generation of instruments providing higher angular resolution will provide. We present first ever resolved images of an Io eruption site taken from the ground, images of Io's Loki Patera taken with Fizeau imaging at the 22.8 meter LBT [Conrad, et al., AJ, 2015]. We will also present preliminary analysis of two data sets acquired during the 2015 opposition: L-band fringes at Kurdalagon and an occultation of Loki and Pele by Europa (see figure). The light curves from this occultation will yield an order of magnitude improvement in spatial resolution along the path of ingress and egress. We will conclude by providing an overview of the overall benefit of recent and future advances in angular resolution for planetary science.

Development of High-Resolution Dynamic Dust Source Function - A Case Study with a Strong Dust Storm in a Regional Model

NASA Technical Reports Server (NTRS)

Kim, Dongchul; Chin, Mian; Kemp, Eric M.; Tao, Zhining; Peters-Lidard, Christa D.; Ginoux, Paul

2017-01-01

A high-resolution dynamic dust source has been developed in the NASA Unified-Weather Research and Forecasting (NU-WRF) model to improve the existing coarse static dust source. In the new dust source map, topographic depression is in 1-km resolution and surface bareness is derived using the Normalized Difference Vegetation Index (NDVI) data from Moderate Resolution Imaging Spectroradiometer (MODIS). The new dust source better resolves the complex topographic distribution over the Western United States where its magnitude is higher than the existing, coarser resolution static source. A case study is conducted with an extreme dust storm that occurred in Phoenix, Arizona in 0203 UTC July 6, 2011. The NU-WRF model with the new high-resolution dynamic dust source is able to successfully capture the dust storm, which was not achieved with the old source identification. However the case study also reveals several challenges in reproducing the time evolution of the short-lived, extreme dust storm events.

Development of High-Resolution Dynamic Dust Source Function -A Case Study with a Strong Dust Storm in a Regional Model

PubMed Central

Kim, Dongchul; Chin, Mian; Kemp, Eric M.; Tao, Zhining; Peters-Lidard, Christa D.; Ginoux, Paul

2018-01-01

A high-resolution dynamic dust source has been developed in the NASA Unified-Weather Research and Forecasting (NU-WRF) model to improve the existing coarse static dust source. In the new dust source map, topographic depression is in 1-km resolution and surface bareness is derived using the Normalized Difference Vegetation Index (NDVI) data from Moderate Resolution Imaging Spectroradiometer (MODIS). The new dust source better resolves the complex topographic distribution over the Western United States where its magnitude is higher than the existing, coarser resolution static source. A case study is conducted with an extreme dust storm that occurred in Phoenix, Arizona in 02-03 UTC July 6, 2011. The NU-WRF model with the new high-resolution dynamic dust source is able to successfully capture the dust storm, which was not achieved with the old source identification. However the case study also reveals several challenges in reproducing the time evolution of the short-lived, extreme dust storm events. PMID:29632432

Development of High-Resolution Dynamic Dust Source Function -A Case Study with a Strong Dust Storm in a Regional Model.

PubMed

Kim, Dongchul; Chin, Mian; Kemp, Eric M; Tao, Zhining; Peters-Lidard, Christa D; Ginoux, Paul

2017-06-01

A high-resolution dynamic dust source has been developed in the NASA Unified-Weather Research and Forecasting (NU-WRF) model to improve the existing coarse static dust source. In the new dust source map, topographic depression is in 1-km resolution and surface bareness is derived using the Normalized Difference Vegetation Index (NDVI) data from Moderate Resolution Imaging Spectroradiometer (MODIS). The new dust source better resolves the complex topographic distribution over the Western United States where its magnitude is higher than the existing, coarser resolution static source. A case study is conducted with an extreme dust storm that occurred in Phoenix, Arizona in 02-03 UTC July 6, 2011. The NU-WRF model with the new high-resolution dynamic dust source is able to successfully capture the dust storm, which was not achieved with the old source identification. However the case study also reveals several challenges in reproducing the time evolution of the short-lived, extreme dust storm events.

Bayesian Deconvolution for Angular Super-Resolution in Forward-Looking Scanning Radar

PubMed Central

Zha, Yuebo; Huang, Yulin; Sun, Zhichao; Wang, Yue; Yang, Jianyu

2015-01-01

Scanning radar is of notable importance for ground surveillance, terrain mapping and disaster rescue. However, the angular resolution of a scanning radar image is poor compared to the achievable range resolution. This paper presents a deconvolution algorithm for angular super-resolution in scanning radar based on Bayesian theory, which states that the angular super-resolution can be realized by solving the corresponding deconvolution problem with the maximum a posteriori (MAP) criterion. The algorithm considers that the noise is composed of two mutually independent parts, i.e., a Gaussian signal-independent component and a Poisson signal-dependent component. In addition, the Laplace distribution is used to represent the prior information about the targets under the assumption that the radar image of interest can be represented by the dominant scatters in the scene. Experimental results demonstrate that the proposed deconvolution algorithm has higher precision for angular super-resolution compared with the conventional algorithms, such as the Tikhonov regularization algorithm, the Wiener filter and the Richardson–Lucy algorithm. PMID:25806871

X-ray spectroscopy with silicon pin and avalanche photo diodes

NASA Technical Reports Server (NTRS)

Desai, U. D.

1992-01-01

Results of an evaluation of silicon P-Intrinsic-N (PIN) photodiodes and Avalanche Photodiodes (APD) for the direct detection of soft x rays from 1 to 20 keV and for the detection of scintillation light output from CsI(TI) for higher x ray energies (30 to 1000 keV) are presented. About one keV resolution was achieved at room temperature for both the PIN and APD detectors for soft x rays (1 to 20 keV). Commercially available, low power (18 mV), low noise, hybrid preamplifiers, were used. These photodiodes were also coupled to CsI(TI) scintillator and obtained about 6 resolution at 662 keV. The photodiode frequency response matches well with the emission spectrum of the CsI(TI) scintillator providing good spectral resolution and a higher signal than NaI(TI) when viewed by conventional photomultipliers. A PIN-CsI(TI) combination provides a low energy threshold of around 60 keV while for the APD-CsI(TI) it is 15 keV.

A Universal Intervention Program Increases Ethnic-Racial Identity Exploration and Resolution to Predict Adolescent Psychosocial Functioning One Year Later.

PubMed

Umaña-Taylor, Adriana J; Kornienko, Olga; Douglass Bayless, Sara; Updegraff, Kimberly A

2018-01-01

Ethnic-racial identity formation represents a key developmental task that is especially salient during adolescence and has been associated with many indices of positive adjustment. The Identity Project intervention, which targeted ethnic-racial identity exploration and resolution, was designed based on the theory that program-induced changes in ethnic-racial identity would lead to better psychosocial adjustment (e.g., global identity cohesion, self-esteem, mental health, academic achievement). Adolescents (N =215; Mage =15.02, SD =.68; 50% female) participated in a small-scale randomized control trial with an attention control group. A cascading mediation model was tested using pre-test and three follow-up assessments (12, 18, and 67 weeks after baseline). The program led to increases in exploration, subsequent increases in resolution and, in turn, higher global identity cohesion, higher self-esteem, lower depressive symptoms, and better grades. Results support the notion that increasing adolescents' ethnic-racial identity can promote positive psychosocial functioning among youth.

High resolution laser patterning of ITO on PET substrate

NASA Astrophysics Data System (ADS)

Zhang, Tao; Liu, Di; Park, Hee K.; Yu, Dong X.; Hwang, David J.

2013-03-01

Cost-effective laser patterning of indium tin oxide (ITO) thin film coated on flexible polyethylene terephthalate (PET) film substrate for touch panel was studied. The target scribing width was set to the order of 10 μm in order to examine issues involved with higher feature resolution. Picosecond-pulsed laser and Q-switched nanosecond-pulsed laser at the wavelength of 532nm were applied for the comparison of laser patterning in picosecond and nanosecond regimes. While relatively superior scribing quality was achieved by picosecond laser, 532 nm wavelength showed a limitation due to weaker absorption in ITO film. In order to seek for cost-effective solution for high resolution ITO scribing, nanosecond laser pulses were applied and performance of 532nm and 1064nm wavelengths were compared. 1064nm wavelength shows relatively better scribing quality due to the higher absorption ratio in ITO film, yet at noticeable substrate damage. Through single pulse based scribing experiments, we inspected that reduced pulse overlapping is preferred in order to minimize the substrate damage during line patterning.

Chemically amplified i-line positive resist for next-generation flat panel display

NASA Astrophysics Data System (ADS)

Lee, Hsing-Chieh; Lu, Ying-Hao; Huang, Shin-Yih; Lan, Wei-Jen; Hanabata, Makoto

2017-03-01

Traditional diazonaphthoquinone (DNQ) positive photoresists are widely used for TFT-LCD array process. Current LTPS technology has more than 600ppi resolution is required for small or middle-sized TFT liquid crystal display panels. One of the ways to enhance resolution is to apply i-line single exposure system instead of traditional g/h/ibroadband exposure system. We have been developing i-line chemically amplified photoresist ECA 200 series for the next generation flat panel display (FPD). ECA 200 consists of three components: a phenol resin, a photo acid generator and dissolution enhancer. We applied two different types of dissolution enhancers with two different kinds of protected groups to our resist materials. As a result, we achieved higher sensitivity, higher resolution, less footing of the resist profile and reduced standing wave effect compared with traditional DNQ photoresists. In addition, we have found further property of photoresist that does not need post exposure bake (PEB) process. This resist has a great advantage at most of current panel plants without PEB process.

Real-Time Nanoscopy by Using Blinking Enhanced Quantum Dots

PubMed Central

Watanabe, Tomonobu M.; Fukui, Shingo; Jin, Takashi; Fujii, Fumihiko; Yanagida, Toshio

2010-01-01

Superresolution optical microscopy (nanoscopy) is of current interest in many biological fields. Superresolution optical fluctuation imaging, which utilizes higher-order cumulant of fluorescence temporal fluctuations, is an excellent method for nanoscopy, as it requires neither complicated optics nor illuminations. However, it does need an impractical number of images for real-time observation. Here, we achieved real-time nanoscopy by modifying superresolution optical fluctuation imaging and enhancing the fluctuation of quantum dots. Our developed quantum dots have higher blinking than commercially available ones. The fluctuation of the blinking improved the resolution when using a variance calculation for each pixel instead of a cumulant calculation. This enabled us to obtain microscopic images with 90-nm and 80-ms spatial-temporal resolution by using a conventional fluorescence microscope without any optics or devices. PMID:20923631

Preliminary Assessment of Microwave Readout Multiplexing Factor

DOE Office of Scientific and Technical Information (OSTI.GOV)

Croce, Mark Philip; Koehler, Katrina Elizabeth; Rabin, Michael W.

2017-01-23

Ultra-high resolution microcalorimeter gamma spectroscopy is a new non-destructive assay technology for measurement of plutonium isotopic composition, with the potential to reduce total measurement uncertainty to a level competitive with destructive analysis methods [1-4]. Achieving this level of performance in practical applications requires not only the energy resolution now routinely achieved with transition-edge sensor microcalorimeter arrays (an order of magnitude better than for germanium detectors) but also high throughput. Microcalorimeter gamma spectrometers have not yet achieved detection efficiency and count rate capability that is comparable to germanium detectors, largely because of limits from existing readout technology. Microcalorimeter detectors must bemore » operated at low temperature to achieve their exceptional energy resolution. Although the typical 100 mK operating temperatures can be achieved with reliable, cryogen-free systems, the cryogenic complexity and heat load from individual readout channels for large sensor arrays is prohibitive. Multiplexing is required for practical systems. The most mature multiplexing technology at present is time-division multiplexing (TDM) [3, 5-6]. In TDM, the sensor outputs are switched by applying bias current to one SQUID amplifier at a time. Transition-edge sensor (TES) microcalorimeter arrays as large as 256 pixels have been developed for X-ray and gamma-ray spectroscopy using TDM technology. Due to bandwidth limits and noise scaling, TDM is limited to a maximum multiplexing factor of approximately 32-40 sensors on one readout line [8]. Increasing the size of microcalorimeter arrays above the kilopixel scale, required to match the throughput of germanium detectors, requires the development of a new readout technology with a much higher multiplexing factor.« less

Attofarad resolution capacitance-voltage measurement of nanometer scale field effect transistors utilizing ambient noise.

PubMed

Gokirmak, Ali; Inaltekin, Hazer; Tiwari, Sandip

2009-08-19

A high resolution capacitance-voltage (C-V) characterization technique, enabling direct measurement of electronic properties at the nanoscale in devices such as nanowire field effect transistors (FETs) through the use of random fluctuations, is described. The minimum noise level required for achieving sub-aF (10(-18) F) resolution, the leveraging of stochastic resonance, and the effect of higher levels of noise are illustrated through simulations. The non-linear DeltaC(gate-source/drain)-V(gate) response of FETs is utilized to determine the inversion layer capacitance (C(inv)) and carrier mobility. The technique is demonstrated by extracting the carrier concentration and effective electron mobility in a nanoscale Si FET with C(inv) = 60 aF.

Facial identification in very low-resolution images simulating prosthetic vision.

PubMed

Chang, M H; Kim, H S; Shin, J H; Park, K S

2012-08-01

Familiar facial identification is important to blind or visually impaired patients and can be achieved using a retinal prosthesis. Nevertheless, there are limitations in delivering the facial images with a resolution sufficient to distinguish facial features, such as eyes and nose, through multichannel electrode arrays used in current visual prostheses. This study verifies the feasibility of familiar facial identification under low-resolution prosthetic vision and proposes an edge-enhancement method to deliver more visual information that is of higher quality. We first generated a contrast-enhanced image and an edge image by applying the Sobel edge detector and blocked each of them by averaging. Then, we subtracted the blocked edge image from the blocked contrast-enhanced image and produced a pixelized image imitating an array of phosphenes. Before subtraction, every gray value of the edge images was weighted as 50% (mode 2), 75% (mode 3) and 100% (mode 4). In mode 1, the facial image was blocked and pixelized with no further processing. The most successful identification was achieved with mode 3 at every resolution in terms of identification index, which covers both accuracy and correct response time. We also found that the subjects recognized a distinctive face especially more accurately and faster than the other given facial images even under low-resolution prosthetic vision. Every subject could identify familiar faces even in very low-resolution images. And the proposed edge-enhancement method seemed to contribute to intermediate-stage visual prostheses.

Fast ultra-wideband microwave spectral scanning utilizing photonic wavelength- and time-division multiplexing.

PubMed

Li, Yihan; Kuse, Naoya; Fermann, Martin

2017-08-07

A high-speed ultra-wideband microwave spectral scanning system is proposed and experimentally demonstrated. Utilizing coherent dual electro-optical frequency combs and a recirculating optical frequency shifter, the proposed system realizes wavelength- and time-division multiplexing at the same time, offering flexibility between scan speed and size, weight and power requirements (SWaP). High-speed spectral scanning spanning from ~1 to 8 GHz with ~1.2 MHz spectral resolution is achieved experimentally within 14 µs. The system can be easily scaled to higher bandwidth coverage, faster scanning speed or finer spectral resolution with suitable hardware.

Line spread functions of blazed off-plane gratings operated in the Littrow mounting

NASA Astrophysics Data System (ADS)

DeRoo, Casey T.; McEntaffer, Randall L.; Miles, Drew M.; Peterson, Thomas J.; Marlowe, Hannah; Tutt, James H.; Donovan, Benjamin D.; Menz, Benedikt; Burwitz, Vadim; Hartner, Gisela; Allured, Ryan; Smith, Randall K.; Günther, Ramses; Yanson, Alex; Vacanti, Giuseppe; Ackermann, Marcelo

2016-04-01

Future soft x-ray (10 to 50 Å) spectroscopy missions require higher effective areas and resolutions to perform critical science that cannot be done by instruments on current missions. An x-ray grating spectrometer employing off-plane reflection gratings would be capable of meeting these performance criteria. Off-plane gratings with blazed groove facets operating in the Littrow mounting can be used to achieve excellent throughput into orders achieving high resolutions. We have fabricated two off-plane gratings with blazed groove profiles via a technique that uses commonly available microfabrication processes, is easily scaled for mass production, and yields gratings customized for a given mission architecture. Both fabricated gratings were tested in the Littrow mounting at the Max Planck Institute for Extraterrestrial Physics (MPE) PANTER x-ray test facility to assess their performance. The line spread functions of diffracted orders were measured, and a maximum resolution of 800±20 is reported. In addition, we also observe evidence of a blaze effect from measurements of relative efficiencies of the diffracted orders.

Subpixel target detection and enhancement in hyperspectral images

NASA Astrophysics Data System (ADS)

Tiwari, K. C.; Arora, M.; Singh, D.

2011-06-01

Hyperspectral data due to its higher information content afforded by higher spectral resolution is increasingly being used for various remote sensing applications including information extraction at subpixel level. There is however usually a lack of matching fine spatial resolution data particularly for target detection applications. Thus, there always exists a tradeoff between the spectral and spatial resolutions due to considerations of type of application, its cost and other associated analytical and computational complexities. Typically whenever an object, either manmade, natural or any ground cover class (called target, endmembers, components or class) gets spectrally resolved but not spatially, mixed pixels in the image result. Thus, numerous manmade and/or natural disparate substances may occur inside such mixed pixels giving rise to mixed pixel classification or subpixel target detection problems. Various spectral unmixing models such as Linear Mixture Modeling (LMM) are in vogue to recover components of a mixed pixel. Spectral unmixing outputs both the endmember spectrum and their corresponding abundance fractions inside the pixel. It, however, does not provide spatial distribution of these abundance fractions within a pixel. This limits the applicability of hyperspectral data for subpixel target detection. In this paper, a new inverse Euclidean distance based super-resolution mapping method has been presented that achieves subpixel target detection in hyperspectral images by adjusting spatial distribution of abundance fraction within a pixel. Results obtained at different resolutions indicate that super-resolution mapping may effectively aid subpixel target detection.

Subwavelength resolution Fourier ptychography with hemispherical digital condensers

NASA Astrophysics Data System (ADS)

Pan, An; Zhang, Yan; Li, Maosen; Zhou, Meiling; Lei, Ming; Yao, Baoli

2018-02-01

Fourier ptychography (FP) is a promising computational imaging technique that overcomes the physical space-bandwidth product (SBP) limit of a conventional microscope by applying angular diversity illuminations. However, to date, the effective imaging numerical aperture (NA) achievable with a commercial LED board is still limited to the range of 0.3-0.7 with a 4×/0.1NA objective due to the constraint of planar geometry with weak illumination brightness and attenuated signal-to-noise ratio (SNR). Thus the highest achievable half-pitch resolution is usually constrained between 500-1000 nm, which cannot fulfill some needs of high-resolution biomedical imaging applications. Although it is possible to improve the resolution by using a higher magnification objective with larger NA instead of enlarging the illumination NA, the SBP is suppressed to some extent, making the FP technique less appealing, since the reduction of field-of-view (FOV) is much larger than the improvement of resolution in this FP platform. Herein, in this paper, we initially present a subwavelength resolution Fourier ptychography (SRFP) platform with a hemispherical digital condenser to provide high-angle programmable plane-wave illuminations of 0.95NA, attaining a 4×/0.1NA objective with the final effective imaging performance of 1.05NA at a half-pitch resolution of 244 nm with a wavelength of 465 nm across a wide FOV of 14.60 mm2 , corresponding to an SBP of 245 megapixels. Our work provides an essential step of FP towards high-NA imaging applications without scarfing the FOV, making it more practical and appealing.

Fluorescent scanning laser ophthalmoscopy for cellular resolution in vivo mouse retinal imaging: benefits and drawbacks of implementing adaptive optics (Conference Presentation)

NASA Astrophysics Data System (ADS)

Zhang, Pengfei; Goswami, Mayank; Pugh, Edward N.; Zawadzki, Robert J.

2016-03-01

Scanning Laser Ophthalmoscopy (SLO) is a very important imaging tool in ophthalmology research. By combing with Adaptive Optics (AO) technique, AO-SLO can correct for ocular aberrations resulting in cellular level resolution, allowing longitudinal studies of single cells morphology in the living eyes. The numerical aperture (NA) sets the optical resolution that can be achieve in the "classical" imaging systems. Mouse eye has more than twice NA of the human eye, thus offering theoretically higher resolution. However, in most SLO based imaging systems the imaging beam size at mouse pupil sets the NA of that instrument, while most of the AO-SLO systems use almost the full NA of the mouse eye. In this report, we first simulated the theoretical resolution that can be achieved in vivo for different imaging beam sizes (different NA), assumingtwo cases: no aberrations and aberrations based on published mouse ocular wavefront data. Then we imaged mouse retinas with our custom build SLO system using different beam sizes to compare these results with theory. Further experiments include comparison of the SLO and AO-SLO systems for imaging different type of fluorescently labeled cells (microglia, ganglion, photoreceptors, etc.). By comparing those results and taking into account systems complexity and ease of use, the benefits and drawbacks of two imaging systems will be discussed.

Multi-resolution model-based traffic sign detection and tracking

NASA Astrophysics Data System (ADS)

Marinas, Javier; Salgado, Luis; Camplani, Massimo

2012-06-01

In this paper we propose an innovative approach to tackle the problem of traffic sign detection using a computer vision algorithm and taking into account real-time operation constraints, trying to establish intelligent strategies to simplify as much as possible the algorithm complexity and to speed up the process. Firstly, a set of candidates is generated according to a color segmentation stage, followed by a region analysis strategy, where spatial characteristic of previously detected objects are taken into account. Finally, temporal coherence is introduced by means of a tracking scheme, performed using a Kalman filter for each potential candidate. Taking into consideration time constraints, efficiency is achieved two-fold: on the one side, a multi-resolution strategy is adopted for segmentation, where global operation will be applied only to low-resolution images, increasing the resolution to the maximum only when a potential road sign is being tracked. On the other side, we take advantage of the expected spacing between traffic signs. Namely, the tracking of objects of interest allows to generate inhibition areas, which are those ones where no new traffic signs are expected to appear due to the existence of a TS in the neighborhood. The proposed solution has been tested with real sequences in both urban areas and highways, and proved to achieve higher computational efficiency, especially as a result of the multi-resolution approach.

GPUs benchmarking in subpixel image registration algorithm

NASA Astrophysics Data System (ADS)

Sanz-Sabater, Martin; Picazo-Bueno, Jose Angel; Micó, Vicente; Ferrerira, Carlos; Granero, Luis; Garcia, Javier

2015-05-01

Image registration techniques are used among different scientific fields, like medical imaging or optical metrology. The straightest way to calculate shifting between two images is using the cross correlation, taking the highest value of this correlation image. Shifting resolution is given in whole pixels which cannot be enough for certain applications. Better results can be achieved interpolating both images, as much as the desired resolution we want to get, and applying the same technique described before, but the memory needed by the system is significantly higher. To avoid memory consuming we are implementing a subpixel shifting method based on FFT. With the original images, subpixel shifting can be achieved multiplying its discrete Fourier transform by a linear phase with different slopes. This method is high time consuming method because checking a concrete shifting means new calculations. The algorithm, highly parallelizable, is very suitable for high performance computing systems. GPU (Graphics Processing Unit) accelerated computing became very popular more than ten years ago because they have hundreds of computational cores in a reasonable cheap card. In our case, we are going to register the shifting between two images, doing the first approach by FFT based correlation, and later doing the subpixel approach using the technique described before. We consider it as `brute force' method. So we will present a benchmark of the algorithm consisting on a first approach (pixel resolution) and then do subpixel resolution approaching, decreasing the shifting step in every loop achieving a high resolution in few steps. This program will be executed in three different computers. At the end, we will present the results of the computation, with different kind of CPUs and GPUs, checking the accuracy of the method, and the time consumed in each computer, discussing the advantages, disadvantages of the use of GPUs.

Low cost digital electronics for isotope analysis with microcalorimeters - final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

W. Hennig

2006-09-11

The overall goal of the Phase I research was to demonstrate that the digital readout electronics and filter algorithms developed by XIA for use with HPGe detectors can be adapted to high precision, cryogenic gamma detectors (microcalorimeters) and not only match the current state of the art in terms of energy resolution, but do so at a significantly reduced cost. This would make it economically feasible to instrument large arrays of microcalorimeters and would also allow automation of the setup, calibration and operation of large numbers of channels through software. We expected, and have demonstrated, that this approach would furthermore » allow much higher count rates than the optimum filter algorithms currently used. In particular, in measurements with a microcalorimeter at LLNL, the adapted Pixie-16 spectrometer achieved an energy resolution of 0.062%, significantly better than the targeted resolution of 0.1% in the Phase I proposal and easily matching resolutions obtained with LLNL readout electronics and optimum filtering (0.066%). The theoretical maximum output count rate for the filter settings used to achieve this resolution is about 120cps. If the filter is adjusted for maximum throughput with an energy resolution of 0.1% or better, rates of 260cps are possible. This is 20-50 times higher than the maximum count rates of about 5cps with optimum filters for this detector. While microcalorimeter measurements were limited to count rates of ~1.3cps due to the strength of available sources, pulser measurements demonstrated that measured energy resolutions were independent of counting rate to output counting rates well in excess of 200cps or more.. We also developed a preliminary hardware design of a spectrometer module, consisting of a digital processing core and several input options that can be implemented on daughter boards. Depending upon the daughter board, the total parts cost per channel ranged between $12 and $27, resulting in projected product prices of $80 to $160 per channel. This demonstrates that a price of $100 per channel is economically very feasible for large microcalorimeter arrays.« less

Robust video super-resolution with registration efficiency adaptation

NASA Astrophysics Data System (ADS)

Zhang, Xinfeng; Xiong, Ruiqin; Ma, Siwei; Zhang, Li; Gao, Wen

2010-07-01

Super-Resolution (SR) is a technique to construct a high-resolution (HR) frame by fusing a group of low-resolution (LR) frames describing the same scene. The effectiveness of the conventional super-resolution techniques, when applied on video sequences, strongly relies on the efficiency of motion alignment achieved by image registration. Unfortunately, such efficiency is limited by the motion complexity in the video and the capability of adopted motion model. In image regions with severe registration errors, annoying artifacts usually appear in the produced super-resolution video. This paper proposes a robust video super-resolution technique that adapts itself to the spatially-varying registration efficiency. The reliability of each reference pixel is measured by the corresponding registration error and incorporated into the optimization objective function of SR reconstruction. This makes the SR reconstruction highly immune to the registration errors, as outliers with higher registration errors are assigned lower weights in the objective function. In particular, we carefully design a mechanism to assign weights according to registration errors. The proposed superresolution scheme has been tested with various video sequences and experimental results clearly demonstrate the effectiveness of the proposed method.

Fuzzy Classification of High Resolution Remote Sensing Scenes Using Visual Attention Features.

PubMed

Li, Linyi; Xu, Tingbao; Chen, Yun

2017-01-01

In recent years the spatial resolutions of remote sensing images have been improved greatly. However, a higher spatial resolution image does not always lead to a better result of automatic scene classification. Visual attention is an important characteristic of the human visual system, which can effectively help to classify remote sensing scenes. In this study, a novel visual attention feature extraction algorithm was proposed, which extracted visual attention features through a multiscale process. And a fuzzy classification method using visual attention features (FC-VAF) was developed to perform high resolution remote sensing scene classification. FC-VAF was evaluated by using remote sensing scenes from widely used high resolution remote sensing images, including IKONOS, QuickBird, and ZY-3 images. FC-VAF achieved more accurate classification results than the others according to the quantitative accuracy evaluation indices. We also discussed the role and impacts of different decomposition levels and different wavelets on the classification accuracy. FC-VAF improves the accuracy of high resolution scene classification and therefore advances the research of digital image analysis and the applications of high resolution remote sensing images.

Fuzzy Classification of High Resolution Remote Sensing Scenes Using Visual Attention Features

PubMed Central

Xu, Tingbao; Chen, Yun

2017-01-01

In recent years the spatial resolutions of remote sensing images have been improved greatly. However, a higher spatial resolution image does not always lead to a better result of automatic scene classification. Visual attention is an important characteristic of the human visual system, which can effectively help to classify remote sensing scenes. In this study, a novel visual attention feature extraction algorithm was proposed, which extracted visual attention features through a multiscale process. And a fuzzy classification method using visual attention features (FC-VAF) was developed to perform high resolution remote sensing scene classification. FC-VAF was evaluated by using remote sensing scenes from widely used high resolution remote sensing images, including IKONOS, QuickBird, and ZY-3 images. FC-VAF achieved more accurate classification results than the others according to the quantitative accuracy evaluation indices. We also discussed the role and impacts of different decomposition levels and different wavelets on the classification accuracy. FC-VAF improves the accuracy of high resolution scene classification and therefore advances the research of digital image analysis and the applications of high resolution remote sensing images. PMID:28761440

High spatial resolution upgrade of the electron cyclotron emission radiometer for the DIII-D tokamak

DOE PAGES

Truong, D. D.; Austin, M. E.

2014-11-01

The 40-channel DIII-D electron cyclotron emission (ECE) radiometer provides measurements of Te(r,t) at the tokamak midplane from optically thick, second harmonic X-mode emission over a frequency range of 83-130 GHz. Heterodyning divides this frequency range into three 2-18 GHz intermediate frequency (IF) bands. The frequency spacing of the radiometer’s channels results in a spatial resolution of ~1-3 cm, depending on local magnetic field and electron temperature. A new high resolution subsystem has been added to the DIII-D ECE radiometer to make sub-centimeter (0.6-0.8 cm) resolution Te measurements. The high resolution subsystem branches off from the regular channels’ IF bands andmore » consists of a microwave switch to toggle between IF bands, a switched filter bank for frequency selectivity, an adjustable local oscillator and mixer for further frequency down-conversion, and a set of eight microwave filters in the 2-4 GHz range. We achieved a higher spatial resolution through the use of a narrower (200 MHz) filter bandwidth and closer spacing between the filters’ center frequencies (250 MHz). This configuration allows for full coverage of the 83-130 GHz frequency range in 2 GHz bands. Depending on the local magnetic field, this translates into a “zoomed-in” analysis of a ~2-4 cm radial region. These high resolution channels will be most useful in the low-field side edge region where modest Te values (1-2 keV) result in a minimum of relativistic broadening. Some expected uses of these channels include mapping the spatial dependence of Alfven eigenmodes, geodesic acoustic modes, and externally applied magnetic perturbations. Initial Te measurements, which demonstrate that the desired resolution is achieved, is presented.« less

Micro-Spec: A High Performance Compact Spectrometer for Submillimeter Astronomy

NASA Technical Reports Server (NTRS)

Hsieh, Wen-Ting; Moseley, Harvey; Stevenson, Thomas; Brown, Ari; Patel, Amil; U-Yen, Kongpop; Ehsan, Negar; Caltado, Giuseppe; Wollock, Edward

2012-01-01

We describe the micro-Spec, an extremely compact high performance spectrometer for the submillimeter and millimeter spectral ranges. We have designed a fully integrated submillimeter spectrometer based on superconducting microstrip technology and fabricated its critical elements. Using low loss transmission lines, we can produce a fully integrated high resolution submillimeter spectrometer on a single four inch Si wafer. A resolution of 500 can readily be achieved with standard fabrication tolerance, higher with phase trimming. All functions of the spectrometer are integrated - light is coupled to the micro strip circuit with a planar antenna, the spectra discrimination is achieved using a synthetic grating, orders are separated using a built-in planar filter, and the light is detected using photon counting Microwave Kinetic Inductance Detectors (MKID). We will discus the design principle of the instrument, describe its technical advantages, and report the progress on the development of the instrument.

Mu-Spec: A High Performance Compact Spectrometer for Submillimeter Astronomy

NASA Technical Reports Server (NTRS)

Hsieh, Wen-Ting; Moseley, Harvey; Stevenson, Thomas; Brown, Ari; Patel, Amil; U-yen, Kongpop; Ehsan, Negar; Cataldo, Giuseppe; Wollack, Ed

2012-01-01

We describe the Mu-Spec, an extremely compact high performance spectrometer for the submillimeter and millimeter spectral ranges. We have designed a fully integrated submillimeter spectrometer based on superconducting microstrip technology and fabricated its critical elements. Using low loss transmission lines, we can produce a fully integrated high resolution submillimeter spectrometer on a single four inch Si wafer. A resolution of 500 can readily be achieved with standard fabrication tolerance, higher with phase trimming. All functions of the spectrometer are integrated - light is coupled to the microstrip circuit with a planar antenna, the spectra discrimination is achieved using a synthetic grating, orders are separated using a built-in planar filter, and the light is detected using photon counting Microwave Kinetic Inductance Detectors (MKID). We will discus the design principle of the instrument, describe its technical advantages, and report the progress on the development of the instrument.

Geo-PET: A novel generic organ-pet for small animal organs and tissues

NASA Astrophysics Data System (ADS)

Sensoy, Levent

Reconstructed tomographic image resolution of small animal PET imaging systems is improving with advances in radiation detector development. However the trend towards higher resolution systems has come with an increase in price and system complexity. Recent developments in the area of solid-state photomultiplication devices like silicon photomultiplier arrays (SPMA) are creating opportunities for new high performance tools for PET scanner design. Imaging of excised small animal organs and tissues has been used as part of post-mortem studies in order to gain detailed, high-resolution anatomical information on sacrificed animals. However, this kind of ex-vivo specimen imaging has largely been limited to ultra-high resolution muCT. The inherent limitations to PET resolution have, to date, excluded PET imaging from these ex-vivo imaging studies. In this work, we leverage the diminishing physical size of current generation SPMA designs to create a very small, simple, and high-resolution prototype detector system targeting ex-vivo tomographic imaging of small animal organs and tissues. We investigate sensitivity, spatial resolution, and the reconstructed image quality of a prototype small animal PET scanner designed specifically for imaging of excised murine tissue and organs. We aim to demonstrate that a cost-effective silicon photomultiplier (SiPM) array based design with thin crystals (2 mm) to minimize depth of interaction errors might be able to achieve sub-millimeter resolution. We hypothesize that the substantial decrease in sensitivity associated with the thin crystals can be compensated for with increased solid angle detection, longer acquisitions, higher activity and wider acceptance energy windows (due to minimal scatter from excised organs). The constructed system has a functional field of view (FoV) of 40 mm diameter, which is adequate for most small animal specimen studies. We perform both analytical (3D-FBP) and iterative (ML-EM) methods in order to reconstruct tomographic images. Results demonstrate good agreement between the simulation and the prototype. Our detector system with pixelated crystals is able to separate small objects as close as 1.25 mm apart, whereas spatial resolution converges to the theoretical limit of 1.6 mm (half the size of the smallest detecting element), which is to comparable to the spatial resolution of the existing commercial small animal PET systems. Better system spatial resolution is achievable with new generation SiPM detector boards with 1 mm x 1 mm cell dimensions. We demonstrate through Monte Carlo simulations that it is possible to achieve sub-millimeter spatial image resolution (0.7 mm for our scanner) in complex objects using monolithic crystals and exploiting the light-sharing mechanism among the neighboring detector cells. Results also suggest that scanner (or object) rotation minimizes artifacts arising from poor angular sampling, which is even more significant in smaller PET designs as the gaps between the sensitive regions of the detector have a more exaggerated effect on the overall reconstructed image quality when the design is more compact. Sensitivity of the system, on the other hand, can be doubled by adding two additional detector heads resulting in a, fully closed, 4? geometry.

A novel super-resolution camera model

NASA Astrophysics Data System (ADS)

Shao, Xiaopeng; Wang, Yi; Xu, Jie; Wang, Lin; Liu, Fei; Luo, Qiuhua; Chen, Xiaodong; Bi, Xiangli

2015-05-01

Aiming to realize super resolution(SR) to single image and video reconstruction, a super resolution camera model is proposed for the problem that the resolution of the images obtained by traditional cameras behave comparatively low. To achieve this function we put a certain driving device such as piezoelectric ceramics in the camera. By controlling the driving device, a set of continuous low resolution(LR) images can be obtained and stored instantaneity, which reflect the randomness of the displacements and the real-time performance of the storage very well. The low resolution image sequences have different redundant information and some particular priori information, thus it is possible to restore super resolution image factually and effectively. The sample method is used to derive the reconstruction principle of super resolution, which analyzes the possible improvement degree of the resolution in theory. The super resolution algorithm based on learning is used to reconstruct single image and the variational Bayesian algorithm is simulated to reconstruct the low resolution images with random displacements, which models the unknown high resolution image, motion parameters and unknown model parameters in one hierarchical Bayesian framework. Utilizing sub-pixel registration method, a super resolution image of the scene can be reconstructed. The results of 16 images reconstruction show that this camera model can increase the image resolution to 2 times, obtaining images with higher resolution in currently available hardware levels.

Hybrid method for building extraction in vegetation-rich urban areas from very high-resolution satellite imagery

NASA Astrophysics Data System (ADS)

Jayasekare, Ajith S.; Wickramasuriya, Rohan; Namazi-Rad, Mohammad-Reza; Perez, Pascal; Singh, Gaurav

2017-07-01

A continuous update of building information is necessary in today's urban planning. Digital images acquired by remote sensing platforms at appropriate spatial and temporal resolutions provide an excellent data source to achieve this. In particular, high-resolution satellite images are often used to retrieve objects such as rooftops using feature extraction. However, high-resolution images acquired over built-up areas are associated with noises such as shadows that reduce the accuracy of feature extraction. Feature extraction heavily relies on the reflectance purity of objects, which is difficult to perfect in complex urban landscapes. An attempt was made to increase the reflectance purity of building rooftops affected by shadows. In addition to the multispectral (MS) image, derivatives thereof namely, normalized difference vegetation index and principle component (PC) images were incorporated in generating the probability image. This hybrid probability image generation ensured that the effect of shadows on rooftop extraction, particularly on light-colored roofs, is largely eliminated. The PC image was also used for image segmentation, which further increased the accuracy compared to segmentation performed on an MS image. Results show that the presented method can achieve higher rooftop extraction accuracy (70.4%) in vegetation-rich urban areas compared to traditional methods.

Enhancing resolution and contrast in second-harmonic generation microscopy using an advanced maximum likelihood estimation restoration method

NASA Astrophysics Data System (ADS)

Sivaguru, Mayandi; Kabir, Mohammad M.; Gartia, Manas Ranjan; Biggs, David S. C.; Sivaguru, Barghav S.; Sivaguru, Vignesh A.; Berent, Zachary T.; Wagoner Johnson, Amy J.; Fried, Glenn A.; Liu, Gang Logan; Sadayappan, Sakthivel; Toussaint, Kimani C.

2017-02-01

Second-harmonic generation (SHG) microscopy is a label-free imaging technique to study collagenous materials in extracellular matrix environment with high resolution and contrast. However, like many other microscopy techniques, the actual spatial resolution achievable by SHG microscopy is reduced by out-of-focus blur and optical aberrations that degrade particularly the amplitude of the detectable higher spatial frequencies. Being a two-photon scattering process, it is challenging to define a point spread function (PSF) for the SHG imaging modality. As a result, in comparison with other two-photon imaging systems like two-photon fluorescence, it is difficult to apply any PSF-engineering techniques to enhance the experimental spatial resolution closer to the diffraction limit. Here, we present a method to improve the spatial resolution in SHG microscopy using an advanced maximum likelihood estimation (AdvMLE) algorithm to recover the otherwise degraded higher spatial frequencies in an SHG image. Through adaptation and iteration, the AdvMLE algorithm calculates an improved PSF for an SHG image and enhances the spatial resolution by decreasing the full-width-at-halfmaximum (FWHM) by 20%. Similar results are consistently observed for biological tissues with varying SHG sources, such as gold nanoparticles and collagen in porcine feet tendons. By obtaining an experimental transverse spatial resolution of 400 nm, we show that the AdvMLE algorithm brings the practical spatial resolution closer to the theoretical diffraction limit. Our approach is suitable for adaptation in micro-nano CT and MRI imaging, which has the potential to impact diagnosis and treatment of human diseases.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xu, C. Shan; Hayworth, Kenneth J.; Lu, Zhiyuan

Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) can automatically generate 3D images with superior z-axis resolution, yielding data that needs minimal image registration and related post-processing. Obstacles blocking wider adoption of FIB-SEM include slow imaging speed and lack of long-term system stability, which caps the maximum possible acquisition volume. Here, we present techniques that accelerate image acquisition while greatly improving FIB-SEM reliability, allowing the system to operate for months and generating continuously imaged volumes > 10 6 ?m 3 . These volumes are large enough for connectomics, where the excellent z resolution can help in tracing of small neuronal processesmore » and accelerate the tedious and time-consuming human proofreading effort. Even higher resolution can be achieved on smaller volumes. We present example data sets from mammalian neural tissue, Drosophila brain, and Chlamydomonas reinhardtii to illustrate the power of this novel high-resolution technique to address questions in both connectomics and cell biology.« less

High Spectral Resolution Lidar for atmospheric temperature profiling.

NASA Astrophysics Data System (ADS)

Razenkov, I.; Eloranta, E. W.

2017-12-01

The High Spectral Resolution Lidar (HSRL) designed at the University of Wisconsin-Madison is equipped with two iodine absorption filters with different line widths (1.8 GHz and 2.85 GHz). The filters are implemented to discriminate between Mie and Rayleigh backscattering and to resolve temperature sensitive changes in Rayleigh spectrum for atmospheric temperature profile measurements. This measurement capability makes the instrument intrinsically and absolutely calibrated. HSRL has a shared transmitter-receiver telescope and operates in the eye-safe mode with the product of laser average power and telescope aperture less than 0.025 𝑊𝑚2 at 532 nm. With this low-power prototype instrument we have achieved temperature profile measurements extending above tropopause with a time resolution of several hours. Further instrument optimizations will reduce systematic measurement errors and will improve a signal-to-noise ratio providing temperature data comparable to a standard radiosonde with higher time resolution.

Enhanced FIB-SEM systems for large-volume 3D imaging.

PubMed

Xu, C Shan; Hayworth, Kenneth J; Lu, Zhiyuan; Grob, Patricia; Hassan, Ahmed M; García-Cerdán, José G; Niyogi, Krishna K; Nogales, Eva; Weinberg, Richard J; Hess, Harald F

2017-05-13

Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) can automatically generate 3D images with superior z-axis resolution, yielding data that needs minimal image registration and related post-processing. Obstacles blocking wider adoption of FIB-SEM include slow imaging speed and lack of long-term system stability, which caps the maximum possible acquisition volume. Here, we present techniques that accelerate image acquisition while greatly improving FIB-SEM reliability, allowing the system to operate for months and generating continuously imaged volumes > 10 6 µm 3 . These volumes are large enough for connectomics, where the excellent z resolution can help in tracing of small neuronal processes and accelerate the tedious and time-consuming human proofreading effort. Even higher resolution can be achieved on smaller volumes. We present example data sets from mammalian neural tissue, Drosophila brain, and Chlamydomonas reinhardtii to illustrate the power of this novel high-resolution technique to address questions in both connectomics and cell biology.

New frontiers in H-Beta auroral photometry

NASA Astrophysics Data System (ADS)

Unick, C.; Donovan, E.; Connors, M. G.; Spanswick, E.; Jackel, B. J.; Greffen, M. J.; Wilson, C.; Little, J.; Chaddock, D.; Schofield, I.; MacRae, A.; Chen, S.; Crowther, A.; James, S.; Read, A.; Willis, T.

2013-12-01

The proton aurora provides valuable information about magnetotail structure and dynamics. For example, the location of the equatorward boundary of the proton aurora is a robust indicator of magnetotail stretching. Also, proton auroral luminosities combined with in situ ion measurements provide important information about magnetic mapping between the inner CPS and the auroral ionosphere. In this paper, we present a new and innovative proton-auroral (H-Beta) meridian-scanning photometer (MSP) capable of higher spatial and temporal resolution than has been achieved in the past. This H-Beta MSP is the first of a new dual-wavelength (signal/background) MSP design with a single scanning mirror and no other moving parts. The novel filtering architecture allows for a near 100% duty cycle with a 30-second meridian scan and configurable operating modes. The new design is significantly more sensitive than the legacy CANOPUS MSPs. The increased SNR can be employed in a variety of ways, such as to achieve significantly higher time resolution. Here, we present the new instrument design, test data from a commissioning campaign in Athabasca, and some thoughts on how the enhance proton auroral capability can increase the science value of these measurements.

Characterizing Time to Diagnostic Resolution After an Abnormal Cancer Screening Exam in Older Adult Participants in the Ohio Patient Navigation Research Program.

PubMed

DeSalvo, Jennifer M; Young, Gregory S; Krok-Schoen, Jessica L; Paskett, Electra D

2017-06-01

This study aims to test the effectiveness of a patient navigation (PN) intervention to reduce time to diagnostic resolution among older adults age ≥65 years versus those <65 years with abnormal breast, cervical, or colorectal cancer screening exams participating in the Ohio Patient Navigation Research Program (OPNRP). The OPNRP utilized a nested cohort group-randomized trial design to randomize 862 participants ( n = 67 for ≥65 years; n = 795 for <65 years) to PN or usual care conditions. A shared frailty Cox model tested the effect of PN on time to resolution. Older adult participants randomized to PN achieved a 6-month resolution rate that was 127% higher than those randomized to usual care ( p = .001). This effect was not significantly different from participants <65 years. PN significantly reduced time to diagnostic resolution among older adults beginning 6 months after an abnormal cancer screening exam. Health care systems should include this population in PN programs to reduce cancer disparities.

Continuous flow electrophoresis: The effect of sample concentration on throughput and resolution in an upward flowing system

NASA Technical Reports Server (NTRS)

Jandebeur, T. S.

1980-01-01

The effect of sample concentration on throughput and resolution in a modified continuous particle electrophoresis (CPE) system with flow in an upward direction is investigated. Maximum resolution is achieved at concentrations ranging from 2 x 10 to the 8th power cells/ml to 8 x 10 to the 8th power cells/ml. The widest peak separation is at 2 x 10 to the 8th power cells/ml; however, the sharpest peaks and least overlap between cell populations is at 8 x 10 to the 8th power cells/ml. Apparently as a result of improved electrophoresis cell performance due to coasting the chamber with bovine serum albumin, changing the electrode membranes and rinse, and lowering buffer temperatures, sedimentation effects attending to higher concentrations are diminished. Throughput as measured by recovery of fixed cells is diminished at the concentrations judged most likely to yield satisfactory resolution. The tradeoff appears to be improved recovery/throughput at the expense of resolution.

Pure optical photoacoustic microscopy

PubMed Central

Xie, Zhixing; Chen, Sung-Liang; Ling, Tao; Guo, L. Jay; Carson, Paul L.; Wang, Xueding

2011-01-01

The concept of pure optical photoacoustic microscopy(POPAM) was proposed based on optical rastering of a focused excitation beam and optically sensing the photoacoustic signal using a microring resonator fabricated by a nanoimprinting technique. After the refinements of the microring’s working wavelength and in the resonator structure and mold fabrication, an ultrahigh Q factor of 3.0×105 was achieved which provided high sensitivity with a noise equivalent detectable pressure(NEDP) value of 29Pa. This NEDP is much lower than the hundreds of Pascals achieved with existing optical resonant structures such as etalons, fiber gratings and dielectric multilayer interference filters available for acoustic measurement. The featured high sensitivity allowed the microring resonator to detect the weak photoacoustic signals from micro- or submicroscale objects. The inherent superbroad bandwidth of the optical microring resonator combined with an optically focused scanning beam provided POPAM with high resolution in the axial as well as both lateral directions while the axial resolution of conventional photoacoustic microscopy (PAM) suffers from the limited bandwidth of PZT detectors. Furthermore, the broadband microring resonator showed similar sensitivity to that of our most sensitive PZT detector. The current POPAM system provides a lateral resolution of 5 μm and an axial resolution of 8 μm, comparable to that achieved by optical microscopy while presenting the unique contrast of optical absorption and functional information complementing other optical modalities. The 3D structure of microvasculature, including capillary networks, and even individual red blood cells have been discerned successfully in the proof-of-concept experiments on mouse bladders ex vivo and mouse ears in vivo. The potential of approximately GHz bandwidth of the microring resonator also might allow much higher resolution than shown here in microscopy of optical absorption and acoustic propagation properties at depths in unfrozen tissue specimens or thicker tissue sections, which is not now imageable with current optical or acoustic microscopes of comparable resolution. PMID:21643156

Assessment of the impact of modeling axial compression on PET image reconstruction.

PubMed

Belzunce, Martin A; Reader, Andrew J

2017-10-01

To comprehensively evaluate both the acceleration and image-quality impacts of axial compression and its degree of modeling in fully 3D PET image reconstruction. Despite being used since the very dawn of 3D PET reconstruction, there are still no extensive studies on the impact of axial compression and its degree of modeling during reconstruction on the end-point reconstructed image quality. In this work, an evaluation of the impact of axial compression on the image quality is performed by extensively simulating data with span values from 1 to 121. In addition, two methods for modeling the axial compression in the reconstruction were evaluated. The first method models the axial compression in the system matrix, while the second method uses an unmatched projector/backprojector, where the axial compression is modeled only in the forward projector. The different system matrices were analyzed by computing their singular values and the point response functions for small subregions of the FOV. The two methods were evaluated with simulated and real data for the Biograph mMR scanner. For the simulated data, the axial compression with span values lower than 7 did not show a decrease in the contrast of the reconstructed images. For span 11, the standard sinogram size of the mMR scanner, losses of contrast in the range of 5-10 percentage points were observed when measured for a hot lesion. For higher span values, the spatial resolution was degraded considerably. However, impressively, for all span values of 21 and lower, modeling the axial compression in the system matrix compensated for the spatial resolution degradation and obtained similar contrast values as the span 1 reconstructions. Such approaches have the same processing times as span 1 reconstructions, but they permit significant reduction in storage requirements for the fully 3D sinograms. For higher span values, the system has a large condition number and it is therefore difficult to recover accurately the higher frequencies. Modeling the axial compression also achieved a lower coefficient of variation but with an increase of intervoxel correlations. The unmatched projector/backprojector achieved similar contrast values to the matched version at considerably lower reconstruction times, but at the cost of noisier images. For a line source scan, the reconstructions with modeling of the axial compression achieved similar resolution to the span 1 reconstructions. Axial compression applied to PET sinograms was found to have a negligible impact for span values lower than 7. For span values up to 21, the spatial resolution degradation due to the axial compression can be almost completely compensated for by modeling this effect in the system matrix at the expense of considerably larger processing times and higher intervoxel correlations, while retaining the storage benefit of compressed data. For even higher span values, the resolution loss cannot be completely compensated possibly due to an effective null space in the system. The use of an unmatched projector/backprojector proved to be a practical solution to compensate for the spatial resolution degradation at a reasonable computational cost but can lead to noisier images. © 2017 The Authors. Medical Physics published by Wiley Periodicals, Inc. on behalf of American Association of Physicists in Medicine.

High resolution imaging of a subsonic projectile using automated mirrors with large aperture

NASA Astrophysics Data System (ADS)

Tateno, Y.; Ishii, M.; Oku, H.

2017-02-01

Visual tracking of high-speed projectiles is required for studying the aerodynamics around the objects. One solution to this problem is a tracking method based on the so-called 1 ms Auto Pan-Tilt (1ms-APT) system that we proposed in previous work, which consists of rotational mirrors and a high-speed image processing system. However, the images obtained with that system did not have high enough resolution to realize detailed measurement of the projectiles because of the size of the mirrors. In this study, we propose a new system consisting of enlarged mirrors for tracking a high-speed projectiles so as to achieve higher-resolution imaging, and we confirmed the effectiveness of the system via an experiment in which a projectile flying at subsonic speed tracked.

Rotational spectroscopy of cold and trapped molecular ions in the Lamb-Dicke regime

NASA Astrophysics Data System (ADS)

Alighanbari, S.; Hansen, M. G.; Korobov, V. I.; Schiller, S.

2018-06-01

Sympathetic cooling of trapped ions has been established as a powerful technique for the manipulation of non-laser-coolable ions1-4. For molecular ions, it promises vastly enhanced spectroscopic resolution and accuracy. However, this potential remains untapped so far, with the best resolution achieved being not better than 5 × 10-8 fractionally, due to residual Doppler broadening being present in ion clusters even at the lowest achievable translational temperatures5. Here we introduce a general and accessible approach that enables Doppler-free rotational spectroscopy. It makes use of the strong radial spatial confinement of molecular ions when trapped and crystallized in a linear quadrupole trap, providing the Lamb-Dicke regime for rotational transitions. We achieve a linewidth of 1 × 10-9 fractionally and 1.3 kHz absolute, an improvement of ≃50-fold over the previous highest resolution in rotational spectroscopy. As an application, we demonstrate the most precise test of ab initio molecular theory and the most accurate (1.3 × 10-9) determination of the proton mass using molecular spectroscopy. The results represent the long overdue extension of Doppler-free microwave spectroscopy of laser-cooled atomic ion clusters6 to higher spectroscopy frequencies and to molecules. This approach enables a wide range of high-accuracy measurements on molecules, both on rotational and, as we project, vibrational transitions.

A device to measure the effects of strong magnetic fields on the image resolution of PET scanners

NASA Astrophysics Data System (ADS)

Burdette, D.; Albani, D.; Chesi, E.; Clinthorne, N. H.; Cochran, E.; Honscheid, K.; Huh, S. S.; Kagan, H.; Knopp, M.; Lacasta, C.; Mikuz, M.; Schmalbrock, P.; Studen, A.; Weilhammer, P.

2009-10-01

Very high resolution images can be achieved in small animal PET systems utilizing solid state silicon pad detectors. As these systems approach sub-millimeter resolutions, the range of the positron is becoming the dominant contribution to image blur. The size of the positron range effect depends on the initial positron energy and hence the radioactive tracer used. For higher energy positron emitters, such as Ga68 and Tc94m, which are gaining importance in small animal studies, the width of the annihilation point distribution dominates the spatial resolution. This positron range effect can be reduced by embedding the field of view of the PET scanner in a strong magnetic field. In order to confirm this effect experimentally, we developed a high resolution PET instrument based on silicon pad detectors that can operate in a 7 T magnetic field. In this paper, we describe the instrument and present initial results of a study of the effects of magnetic fields up to 7 T on PET image resolution for Na22 and Ga68 point sources.

Multiple-aperture optical design for micro-level cameras using 3D-printing method

NASA Astrophysics Data System (ADS)

Peng, Wei-Jei; Hsu, Wei-Yao; Cheng, Yuan-Chieh; Lin, Wen-Lung; Yu, Zong-Ru; Chou, Hsiao-Yu; Chen, Fong-Zhi; Fu, Chien-Chung; Wu, Chong-Syuan; Huang, Chao-Tsung

2018-02-01

The design of the ultra miniaturized camera using 3D-printing technology directly printed on to the complementary metal-oxide semiconductor (CMOS) imaging sensor is presented in this paper. The 3D printed micro-optics is manufactured using the femtosecond two-photon direct laser writing, and the figure error which could achieve submicron accuracy is suitable for the optical system. Because the size of the micro-level camera is approximately several hundreds of micrometers, the resolution is reduced much and highly limited by the Nyquist frequency of the pixel pitch. For improving the reduced resolution, one single-lens can be replaced by multiple-aperture lenses with dissimilar field of view (FOV), and then stitching sub-images with different FOV can achieve a high resolution within the central region of the image. The reason is that the angular resolution of the lens with smaller FOV is higher than that with larger FOV, and then the angular resolution of the central area can be several times than that of the outer area after stitching. For the same image circle, the image quality of the central area of the multi-lens system is significantly superior to that of a single-lens. The foveated image using stitching FOV breaks the limitation of the resolution for the ultra miniaturized imaging system, and then it can be applied such as biomedical endoscopy, optical sensing, and machine vision, et al. In this study, the ultra miniaturized camera with multi-aperture optics is designed and simulated for the optimum optical performance.

Enhancing sensitivity of high resolution optical coherence tomography using an optional spectrally encoded extended source (Conference Presentation)

NASA Astrophysics Data System (ADS)

Yu, Xiaojun; Liu, Xinyu; Chen, Si; Wang, Xianghong; Liu, Linbo

2016-03-01

High-resolution optical coherence tomography (OCT) is of critical importance to disease diagnosis because it is capable of providing detailed microstructural information of the biological tissues. However, a compromise usually has to be made between its spatial resolutions and sensitivity due to the suboptimal spectral response of the system components, such as the linear camera, the dispersion grating, and the focusing lenses, etc. In this study, we demonstrate an OCT system that achieves both high spatial resolutions and enhanced sensitivity through utilizing a spectrally encoded source. The system achieves a lateral resolution of 3.1 μm and an axial resolution of 2.3 μm in air; when with a simple dispersive prism placed in the infinity space of the sample arm optics, the illumination beam on the sample is transformed into a line source with a visual angle of 10.3 mrad. Such an extended source technique allows a ~4 times larger maximum permissible exposure (MPE) than its point source counterpart, which thus improves the system sensitivity by ~6dB. In addition, the dispersive prism can be conveniently switched to a reflector. Such flexibility helps increase the penetration depth of the system without increasing the complexity of the current point source devices. We conducted experiments to characterize the system's imaging capability using the human fingertip in vivo and the swine eye optic never disc ex vivo. The higher penetration depth of such a system over the conventional point source OCT system is also demonstrated in these two tissues.

High Spectral Resolution, High Cadence, Imaging X-Ray Microcalorimeters for Solar Physics

NASA Technical Reports Server (NTRS)

Bandler, Simon R.; Bailey, Catherine N.; Bookbinder, Jay A.; DeLuca, Edward E.; Chervenak, Jay A.; Eckart, Megan E.; Finkbeiner, Fred M.; Kelley, Daniel P.; Kelley, Richard L.; Kilbourne, Caroline A.;

Estimation of position resolution for DOI-PET detector using diameter 0.2 mm WLS fibers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kaneko, Naomi; Ito, Hiroshi; Kawai, Hideyuki

We have been developing sub mm resolution andmore » $$ 1 million DOI-PET detector using wavelength shifting fibers (WLSF), scintillation crystals of plate shape and SiPM (MPPC: HAMAMATSU K. K.). Conventional design of DOI-PET detector is obtained about mm{sup 3} of resolution by using some blocks detecting gamma-ray in mm 3 voxel. It requires the production cost of $$ a few ten million or more for high technique of processing crystal and a lot of number of photo-devices, and this technology is reaching the limit of the resolution. Both higher resolution and lower cost of DOI-PET detector production is challenging for PET diagnosis population. We propose two type of detector. One is a whole body PET system, and the other for brain or small animal. Both PET system consist 6 blocks. the former consist of 6 layers 300 mm x 300 mm x 4 mm crystal plate. The latter consist 16 crystal layers, 4 x 4 crystal array. The size of crystal plate is 40 mm x 40 mm x 1 mm.The WLSF sheets connect to upper and lower plane. The whole PET systems connect 8 SiPMs are bonded on each side. For the brain PET, 9 WLSF fibers are bond on the each side. The expected position resolution maybe less than 1 mm at the former. We have estimation experimental performance the system using {sup 22}Na radioactive source. The collection efficiency of WLSF (R-3) sheet was achieved 10% with GAGG at 511 keV. The relation between reconstruction position and incident position is obtained linearity and achieved the resolution of 0.7 mm FWHM for x-axis of DOI by readout WLSF. (authors)« less

A silicon surface barrier telescope for solar particles identification

NASA Technical Reports Server (NTRS)

Sequeiros, J.; Medina, J.

1985-01-01

From the results three conclusions can be made: (1) the detector system described and tested is capable of good charge resolution from He to Al although beyond Ne the statistic is very poor; (2) in the high gain mode, isotopic resolution has been achieved for Li-6/Li-7 Be-7/Be-9; (3) the much higher yield of He over He and of Be-9 over Be-10 in these types of nuclear reactions prevents obtaining experimental evidence of those isotopes, although it is believed that, at least He-3/He-4 can be resolved under other more favorable conditions (i.e., solar He-3-rich events).

Improving X-Ray Optics via Differential Deposition

NASA Technical Reports Server (NTRS)

Kilaru, Kiranmayee; Ramsey, Brian D.; Atkins, Carolyn

2017-01-01

Differential deposition, a post-fabrication figure correction technique, has the potential to significantly improve the imaging quality of grazing-incidence X-ray optics. DC magnetron sputtering is used to selectively coat the mirror in order to minimize the figure deviations. Custom vacuum chambers have been developed at NASA MSFC that will enable the implementation of the deposition on X-ray optics. A factor of two improvement has been achieved in the angular resolution of the full-shell X-ray optics with first stage correction of differential deposition. Current efforts are focused on achieving higher improvements through efficient implementation of differential deposition.

High-resolution separation of neodymium and dysprosium ions utilizing extractant-impregnated graft-type particles.

PubMed

Uchiyama, Shoichiro; Sasaki, Takaaki; Ishihara, Ryo; Fujiwara, Kunio; Sugo, Takanobu; Umeno, Daisuke; Saito, Kyoichi

2018-01-19

An efficient method for rare metal recovery from environmental water and urban mines is in high demand. Toward rapid and high-resolution rare metal ion separation, a novel bis(2-ethylhexyl) phosphate (HDEHP)-impregnated graft-type particle as a filler for a chromatography column is proposed. To achieve rapid and high-resolution separation, a convection-flow-aided elution mode is required. The combination of 35 μm non-porous particles and a polymer-brush-rich particle structure minimizes the distance from metal ion binding sites to the convection flow in the column, resulting in minimized diffusional mass transfer resistance and the convection-flow-aided elution mode. The HDEHP-impregnated graft-type non-porous-particle-packed cartridge developed in this study exhibited a higher separation performance for model rare metals, neodymium (III) and dysprosium (III) ions, and a narrower peak at a higher linear velocity, than those of previous HDEHP-impregnated fiber-packed and commercially available Lewatit ® VP OC 1026-packed cartridges. Copyright © 2017 Elsevier B.V. All rights reserved.

High-resolution whole-brain DCE-MRI using constrained reconstruction: Prospective clinical evaluation in brain tumor patients.

PubMed

Guo, Yi; Lebel, R Marc; Zhu, Yinghua; Lingala, Sajan Goud; Shiroishi, Mark S; Law, Meng; Nayak, Krishna

2016-05-01

To clinically evaluate a highly accelerated T1-weighted dynamic contrast-enhanced (DCE) MRI technique that provides high spatial resolution and whole-brain coverage via undersampling and constrained reconstruction with multiple sparsity constraints. Conventional (rate-2 SENSE) and experimental DCE-MRI (rate-30) scans were performed 20 minutes apart in 15 brain tumor patients. The conventional clinical DCE-MRI had voxel dimensions 0.9 × 1.3 × 7.0 mm(3), FOV 22 × 22 × 4.2 cm(3), and the experimental DCE-MRI had voxel dimensions 0.9 × 0.9 × 1.9 mm(3), and broader coverage 22 × 22 × 19 cm(3). Temporal resolution was 5 s for both protocols. Time-resolved images and blood-brain barrier permeability maps were qualitatively evaluated by two radiologists. The experimental DCE-MRI scans showed no loss of qualitative information in any of the cases, while achieving substantially higher spatial resolution and whole-brain spatial coverage. Average qualitative scores (from 0 to 3) were 2.1 for the experimental scans and 1.1 for the conventional clinical scans. The proposed DCE-MRI approach provides clinically superior image quality with higher spatial resolution and coverage than currently available approaches. These advantages may allow comprehensive permeability mapping in the brain, which is especially valuable in the setting of large lesions or multiple lesions spread throughout the brain.

Wide field video-rate two-photon imaging by using spinning disk beam scanner

NASA Astrophysics Data System (ADS)

Maeda, Yasuhiro; Kurokawa, Kazuo; Ito, Yoko; Wada, Satoshi; Nakano, Akihiko

2018-02-01

The microscope technology with wider view field, deeper penetration depth, higher spatial resolution and higher imaging speed are required to investigate the intercellular dynamics or interactions of molecules and organs in cells or a tissue in more detail. The two-photon microscope with a near infrared (NIR) femtosecond laser is one of the technique to improve the penetration depth and spatial resolution. However, the video-rate or high-speed imaging with wide view field is difficult to perform with the conventional two-photon microscope. Because point-to-point scanning method is used in conventional one, so it's difficult to achieve video-rate imaging. In this study, we developed a two-photon microscope with spinning disk beam scanner and femtosecond NIR fiber laser with around 10 W average power for the microscope system to achieve above requirements. The laser is consisted of an oscillator based on mode-locked Yb fiber laser, a two-stage pre-amplifier, a main amplifier based on a Yb-doped photonic crystal fiber (PCF), and a pulse compressor with a pair of gratings. The laser generates a beam with maximally 10 W average power, 300 fs pulse width and 72 MHz repetition rate. And the beam incident to a spinning beam scanner (Yokogawa Electric) optimized for two-photon imaging. By using this system, we achieved to obtain the 3D images with over 1mm-penetration depth and video-rate image with 350 x 350 um view field from the root of Arabidopsis thaliana.

Comparing high-resolution microscopy techniques for potential intraoperative use in guiding low-grade glioma resections.

PubMed

Meza, Daphne; Wang, Danni; Wang, Yu; Borwege, Sabine; Sanai, Nader; Liu, Jonathan T C

2015-04-01

Fluorescence image-guided surgery (FIGS), with contrast provided by 5-ALA-induced PpIX, has been shown to enable a higher extent of resection of high-grade gliomas. However, conventional FIGS with low-power microscopy lacks the sensitivity to aid in low-grade glioma (LGG) resection because PpIX signal is weak and sparse in such tissues. Intraoperative high-resolution microscopy of PpIX fluorescence has been proposed as a method to guide LGG resection, where sub-cellular resolution allows for the visualization of sparse and punctate mitochondrial PpIX production in tumor cells. Here, we assess the performance of three potentially portable high-resolution microscopy techniques that may be used for the intraoperative imaging of human LGG tissue samples with PpIX contrast: high-resolution fiber-optic microscopy (HRFM), high-resolution wide-field microscopy (WFM), and dual-axis confocal (DAC) microscopy. Thick unsectioned human LGG tissue samples (n = 7) with 5-ALA-induced PpIX contrast were imaged using three imaging techniques (HRFM, WFM, DAC). The average signal-to-background ratio (SBR) was then calculated for each imaging modality (5 images per tissue, per modality). HRFM provides the ease of use and portability of a flexible fiber bundle, and is simple and inexpensive to build. However, in most cases (6/7), HRFM is not capable of detecting PpIX signal from LGGs due to high autofluorescence, generated by the fiber bundle under laser illumination at 405 nm, which overwhelms the PpIX signal and impedes its visualization. WFM is a camera-based method possessing high lateral resolution but poor axial resolution, resulting in sub-optimal image contrast. Consistent successful detection of PpIX signal throughout our human LGG tissue samples (n = 7), with an acceptable image contrast (SBR >2), was only achieved using DAC microscopy, which offers superior image resolution and contrast that is comparable to histology, but requires a laser-scanning mechanism to achieve optical sectioning. © 2015 Wiley Periodicals, Inc.

Use of vancomycin silica stationary phase in packed capillary electrochromatography: III. enantiomeric separation of basic compounds with the polar organic mobile phase.

PubMed

Fanali, Salvatore; Catarcini, Paolo; Quaglia, Maria Giovanna

2002-02-01

The separation of basic compounds into their enantiomers was achieved using capillary electrochromatography in 50 or 75 microm inner diameter (ID) fused-silica capillaries packed with silica a stationary phase derivatized with vancomycin and mobile phases composed of mixtures of polar organic solvents containing 13 mM ammonium acetate. Enantiomer resolution, electroosmotic flow, and the number of theoretical plates were strongly influenced by the type and concentration of the organic solvent. Mobile phases composed of 13 mM ammonium acetate dissolved in mixtures of acetonitrile/methanol, ethanol, n-propanol, or isopropanol were tested and the highest enantioresolutions were achieved using the first mobile phase, allowing the separation of almost all investigated enantiomers (9 from 11 basic compounds). The use of capillaries with different ID (50 and 75 microm ID) packed with the same chiral stationary phase revealed that a higher number of theoretical plates and higher enantioresolution was achieved with the tube with lowest ID.

TU-E-217BCD-06: Cone Beam Breast CT with a High Resolution Flat Panel Detector-Improvement of Calcification Visibility.

PubMed

Shen, Y; Zhong, Y; Lai, C; Wang, T; Shaw, C

2012-06-01

To investigate the advantage of a high resolution flat panel detector for improving the visibility of microcalcifications (MCs) in cone beam breast CT Methods: A paraffin cylinder was used to simulate a 100% adipose breast. Calcium carbonate grains, ranging from 125-140 μm to 224 - 250 μm in size, were used to simulate the MCs. Groups of 25 same size MCs were embedded at the phantom center. The phantom was scanned with a bench-top CBCT system at various exposure levels. A 75μm pitch flat panel detector (Dexela 2923, Perkin Elmer) with 500μm thick CsI scintillator plate was used as the high resolution detector. A 194 μm pitch detector (Paxscan 4030CB, Varian Medical Systems) was used for reference. 300 projection images were acquired over 360° and reconstructed. The images were reviewed by 6 readers. The MC visibility was quantified as the fraction of visible MCs and averaged for comparison. The visibility was plotted as a function of the estimated dose level for various MC sizes and detectors. The MTFs and DQEs were measured and compared. For imaging small (200 μm and smaller) MCs, the visibility achieved with the 75μm pitch detector was found to be significantly higher than those achieved with the 194μm pitch detector. For imaging larger MCs, there was little advantage in using the 75μm pitch detector. Using the 75μm pitch detector, MCs as small as 180 μm could be imaged to achieve a visibility of 78% with an isocenter tissue dose of ∼20 mGys versus 62% achieved with the 194 μm pitch detector at the same dose level. It was found that a high pitch flat panel detector had the advantages of extending its imaging capability to higher frequencies thus helping improve the visibility when used to image small MCs. This work was supported in part by grants CA104759, CA13852 and CA124585 from NIH-NCI, a grant EB00117 from NIH-NIBIB, and a subcontract from NIST-ATP. © 2012 American Association of Physicists in Medicine.

Airborne SAR systems for infrastructures monitoring

NASA Astrophysics Data System (ADS)

Perna, Stefano; Berardino, Paolo; Esposito, Carmen; Natale, Antonio

2017-04-01

The present contribution is aimed at showing the capabilities of Synthetic Aperture Radar (SAR) systems mounted onboard airborne platforms for the monitoring of infrastructures. As well known, airborne SAR systems guarantee narrower spatial coverage than satellite sensors [1]. On the other side, airborne SAR products are characterized by geometric resolution typically higher than that achievable in the satellite case, where larger antennas must be necessarily exploited. More important, airborne SAR platforms guarantee operational flexibility significantly higher than that achievable with satellite systems. Indeed, the revisit time between repeated SAR acquisitions in the satellite case cannot be freely decided, whereas in the airborne case it can be kept very short. This renders the airborne platforms of key interest for the monitoring of infrastructures, especially in case of emergencies. However, due to the platform deviations from a rectilinear, reference flight track, the generation of airborne SAR products is not a turn of the crank procedure as in the satellite case. Notwithstanding proper algorithms exist in order to circumvent this kind of limitations. In this work, we show how the exploitation of airborne SAR sensors, coupled to the use of such algorithms, allows obtaining high resolution monitoring of infrastructures in urban areas. [1] G. Franceschetti, and R.Lanari, Synthetic Aperture Radar Processing, CRC PRESS, New York, 1999.

Texton-based super-resolution for achieving high spatiotemporal resolution in hybrid camera system

NASA Astrophysics Data System (ADS)

Kamimura, Kenji; Tsumura, Norimichi; Nakaguchi, Toshiya; Miyake, Yoichi

2010-05-01

Many super-resolution methods have been proposed to enhance the spatial resolution of images by using iteration and multiple input images. In a previous paper, we proposed the example-based super-resolution method to enhance an image through pixel-based texton substitution to reduce the computational cost. In this method, however, we only considered the enhancement of a texture image. In this study, we modified this texton substitution method for a hybrid camera to reduce the required bandwidth of a high-resolution video camera. We applied our algorithm to pairs of high- and low-spatiotemporal-resolution videos, which were synthesized to simulate a hybrid camera. The result showed that the fine detail of the low-resolution video can be reproduced compared with bicubic interpolation and the required bandwidth could be reduced to about 1/5 in a video camera. It was also shown that the peak signal-to-noise ratios (PSNRs) of the images improved by about 6 dB in a trained frame and by 1.0-1.5 dB in a test frame, as determined by comparison with the processed image using bicubic interpolation, and the average PSNRs were higher than those obtained by the well-known Freeman’s patch-based super-resolution method. Compared with that of the Freeman’s patch-based super-resolution method, the computational time of our method was reduced to almost 1/10.

Application of the correlation constrained multivariate curve resolution alternating least-squares method for analyte quantitation in the presence of unexpected interferences using first-order instrumental data.

PubMed

Goicoechea, Héctor C; Olivieri, Alejandro C; Tauler, Romà

2010-03-01

Correlation constrained multivariate curve resolution-alternating least-squares is shown to be a feasible method for processing first-order instrumental data and achieve analyte quantitation in the presence of unexpected interferences. Both for simulated and experimental data sets, the proposed method could correctly retrieve the analyte and interference spectral profiles and perform accurate estimations of analyte concentrations in test samples. Since no information concerning the interferences was present in calibration samples, the proposed multivariate calibration approach including the correlation constraint facilitates the achievement of the so-called second-order advantage for the analyte of interest, which is known to be present for more complex higher-order richer instrumental data. The proposed method is tested using a simulated data set and two experimental data systems, one for the determination of ascorbic acid in powder juices using UV-visible absorption spectral data, and another for the determination of tetracycline in serum samples using fluorescence emission spectroscopy.

Wavelength-encoded tomography based on optical temporal Fourier transform

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Chi; Wong, Kenneth K. Y., E-mail: kywong@eee.hku.hk

We propose and demonstrate a technique called wavelength-encoded tomography (WET) for non-invasive optical cross-sectional imaging, particularly beneficial in biological system. The WET utilizes time-lens to perform the optical Fourier transform, and the time-to-wavelength conversion generates a wavelength-encoded image of optical scattering from internal microstructures, analogous to the interferometery-based imaging such as optical coherence tomography. Optical Fourier transform, in principle, comes with twice as good axial resolution over the electrical Fourier transform, and will greatly simplify the digital signal processing after the data acquisition. As a proof-of-principle demonstration, a 150 -μm (ideally 36 μm) resolution is achieved based on a 7.5-nm bandwidth swept-pump,more » using a conventional optical spectrum analyzer. This approach can potentially achieve up to 100-MHz or even higher frame rate with some proven ultrafast spectrum analyzer. We believe that this technique is innovative towards the next-generation ultrafast optical tomographic imaging application.« less

Building Practical Apertureless Scanning Near-Field Microscopy

NASA Astrophysics Data System (ADS)

Gungordu, M. Zeki

The fundamental objective of this study is to establish a functional, practical apertureless type scanning near-field optical microscope, and to figure out the working mechanism behind it. Whereas a far-field microscope can measure the propagating field's components, this gives us little information about the features of the sample. The resolution is limited to about half of the wavelength of the illuminating light. On the other hand, the a-SNOM system enables achieving non-propagating components of the field, which provides more details about the sample's features. It is really difficult to measure because the amplitude of this field decays exponentially when the tip is moved away from the sample. The sharpness of the tip is the only limitation for resolution of the a-SNOM system. Consequently, the sharp tips are achieved by using electrochemical etching, and these tips are used to detect near-field signal. Separating the weak a-SNOM system signals from the undesired background signal, the higher demodulation background suppression is utilized by lock-in detection.

Recent advancements in structured-illumination microscopy toward live-cell imaging.

PubMed

Hirano, Yasuhiro; Matsuda, Atsushi; Hiraoka, Yasushi

2015-08-01

Fluorescence microscopy allows us to observe fluorescently labeled molecules in diverse biological processes and organelle structures within living cells. However, the diffraction limit restricts its spatial resolution to about half of its wavelength, limiting the capability of biological observation at the molecular level. Structured-illumination microscopy (SIM), a type of super-resolution microscopy, doubles the spatial resolution in all three dimensions by illuminating the sample with a patterned excitation light, followed by computer reconstruction. SIM uses a relatively low illumination power compared with other methods of super-resolution microscopy and is easily available for multicolor imaging. SIM has great potential for meeting the requirements of live-cell imaging. Recent developments in diverse types of SIM have achieved higher spatial (∼50 nm lateral) and temporal (∼100 Hz) resolutions. Here, we review recent advancements in SIM and discuss its application in noninvasive live-cell imaging. © The Author 2015. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array.

PubMed

Bishara, Waheb; Sikora, Uzair; Mudanyali, Onur; Su, Ting-Wei; Yaglidere, Oguzhan; Luckhart, Shirley; Ozcan, Aydogan

2011-04-07

We report a portable lensless on-chip microscope that can achieve <1 µm resolution over a wide field-of-view of ∼ 24 mm(2) without the use of any mechanical scanning. This compact on-chip microscope weighs ∼ 95 g and is based on partially coherent digital in-line holography. Multiple fiber-optic waveguides are butt-coupled to light emitting diodes, which are controlled by a low-cost micro-controller to sequentially illuminate the sample. The resulting lensfree holograms are then captured by a digital sensor-array and are rapidly processed using a pixel super-resolution algorithm to generate much higher resolution holographic images (both phase and amplitude) of the objects. This wide-field and high-resolution on-chip microscope, being compact and light-weight, would be important for global health problems such as diagnosis of infectious diseases in remote locations. Toward this end, we validate the performance of this field-portable microscope by imaging human malaria parasites (Plasmodium falciparum) in thin blood smears. Our results constitute the first-time that a lensfree on-chip microscope has successfully imaged malaria parasites.

Focal Plane Detectors for the Advanced Gamma-Ray Imaging System (AGIS)

NASA Astrophysics Data System (ADS)

Wagner, Robert G.; AGIS Photodetector Group; Byrum, K.; Drake, G.; Falcone, A.; Funk, S.; Horan, D.; Mukherjee, R.; Tajima, H.; Williams, D.

2008-03-01

The Advanced Gamma-Ray Imaging System (AGIS) is a concept for the next generation observatory in ground-based very high energy gamma-ray astronomy. It is being designed to achieve a significant improvement in sensitivity compared to current Imaging Air Cherenkov Telescope (IACT) Arrays. One of the main requirements in order that AGIS fulfill this goal will be to achieve higher angular resolution than current IACTs. Simulations show that a substantial improvement in angular resolution may be achieved if the pixel size is reduced to less than 0.05 deg, i.e. two to three times smaller than the pixel size of current IACT cameras. With finer pixelation and the plan to deploy on the order of 100 telescopes in the AGIS array, the channel count will exceed 1,000,000 imaging pixels. High uniformity and long mean time-to-failure will be important aspects of a successful photodetector technology choice. Here we present alternatives being considered for AGIS, including both silicon photomultipliers (SiPMs) and multi-anode photomultipliers (MAPMTs). Results from laboratory testing of MAPMTs and SiPMs are presented along with results from the first incorporation of these devices in cameras on test bed Cherenkov telescopes.

Tablet disintegration studied by high-resolution real-time magnetic resonance imaging.

PubMed

Quodbach, Julian; Moussavi, Amir; Tammer, Roland; Frahm, Jens; Kleinebudde, Peter

2014-01-01

The present work employs recent advances in high-resolution real-time magnetic resonance imaging (MRI) to investigate the disintegration process of tablets containing disintegrants. A temporal resolution of 75 ms and a spatial resolution of 80 × 80 µm with a section thickness of only 600 µm were achieved. The histograms of MRI videos were quantitatively analyzed with MATLAB. The mechanisms of action of six commercially available disintegrants, the influence of relative tablet density, and the impact of disintegrant concentration were examined. Crospovidone seems to be the only disintegrant acting by a shape memory effect, whereas the others mainly swell. A higher relative density of tablets containing croscarmellose sodium leads to a more even distribution of water within the tablet matrix but hardly impacts the disintegration kinetics. Increasing the polacrilin potassium disintegrant concentration leads to a quicker and more thorough disintegration process. Real-time MRI emerges as valuable tool to visualize and investigate the process of tablet disintegration.

Enhanced FIB-SEM systems for large-volume 3D imaging

PubMed Central

Xu, C Shan; Hayworth, Kenneth J; Lu, Zhiyuan; Grob, Patricia; Hassan, Ahmed M; García-Cerdán, José G; Niyogi, Krishna K; Nogales, Eva; Weinberg, Richard J; Hess, Harald F

2017-01-01

Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) can automatically generate 3D images with superior z-axis resolution, yielding data that needs minimal image registration and related post-processing. Obstacles blocking wider adoption of FIB-SEM include slow imaging speed and lack of long-term system stability, which caps the maximum possible acquisition volume. Here, we present techniques that accelerate image acquisition while greatly improving FIB-SEM reliability, allowing the system to operate for months and generating continuously imaged volumes > 106 µm3. These volumes are large enough for connectomics, where the excellent z resolution can help in tracing of small neuronal processes and accelerate the tedious and time-consuming human proofreading effort. Even higher resolution can be achieved on smaller volumes. We present example data sets from mammalian neural tissue, Drosophila brain, and Chlamydomonas reinhardtii to illustrate the power of this novel high-resolution technique to address questions in both connectomics and cell biology. DOI: http://dx.doi.org/10.7554/eLife.25916.001 PMID:28500755

A novel method for enhancing the lateral resolution and image SNR in confocal microscopy

NASA Astrophysics Data System (ADS)

Chen, Youhua; Zhu, Dazhao; Fang, Yue; Kuang, Cuifang; Liu, Xu

2017-12-01

There is always a tradeoff between the resolution and the signal-to-noise ratio (SNR) in confocal microscopy. In particular, the pinhole size is very important for maintaining a balance between them. In this paper, we propose a method for improving the lateral resolution and image SNR in confocal microscopy without making any changes to the hardware. By using the fluorescence emission difference (FED) approach, we divide the images acquired by different pinhole sizes into one image acquired by the central pinhole and several images acquired by ring-shaped pinholes. Then, they are added together with the deconvolution method. Simulation and experimental results for fluorescent particles and cells show that our method can achieve a far better resolution than a large pinhole and a higher SNR than a small pinhole. Moreover, our method can improve the performance of classic confocal laser scanning microscopy (CLSM) to a certain extent, especially CLSM with a continuously variable pinhole.

Time-frequency model for echo-delay resolution in wideband biosonar.

PubMed

Neretti, Nicola; Sanderson, Mark I; Intrator, Nathan; Simmons, James A

2003-04-01

A time/frequency model of the bat's auditory system was developed to examine the basis for the fine (approximately 2 micros) echo-delay resolution of big brown bats (Eptesicus fuscus), and its performance at resolving closely spaced FM sonar echoes in the bat's 20-100-kHz band at different signal-to-noise ratios was computed. The model uses parallel bandpass filters spaced over this band to generate envelopes that individually can have much lower bandwidth than the bat's ultrasonic sonar sounds and still achieve fine delay resolution. Because fine delay separations are inside the integration time of the model's filters (approximately 250-300 micros), resolving them means using interference patterns along the frequency dimension (spectral peaks and notches). The low bandwidth content of the filter outputs is suitable for relay of information to higher auditory areas that have intrinsically poor temporal response properties. If implemented in fully parallel analog-digital hardware, the model is computationally extremely efficient and would improve resolution in military and industrial sonar receivers.

Enhanced FIB-SEM systems for large-volume 3D imaging

DOE PAGES

Xu, C. Shan; Hayworth, Kenneth J.; Lu, Zhiyuan; ...

2017-05-13

Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) can automatically generate 3D images with superior z-axis resolution, yielding data that needs minimal image registration and related post-processing. Obstacles blocking wider adoption of FIB-SEM include slow imaging speed and lack of long-term system stability, which caps the maximum possible acquisition volume. Here, we present techniques that accelerate image acquisition while greatly improving FIB-SEM reliability, allowing the system to operate for months and generating continuously imaged volumes > 10 6 ?m 3 . These volumes are large enough for connectomics, where the excellent z resolution can help in tracing of small neuronal processesmore » and accelerate the tedious and time-consuming human proofreading effort. Even higher resolution can be achieved on smaller volumes. We present example data sets from mammalian neural tissue, Drosophila brain, and Chlamydomonas reinhardtii to illustrate the power of this novel high-resolution technique to address questions in both connectomics and cell biology.« less

Molecular dynamics-based refinement and validation for sub-5 Å cryo-electron microscopy maps.

PubMed

Singharoy, Abhishek; Teo, Ivan; McGreevy, Ryan; Stone, John E; Zhao, Jianhua; Schulten, Klaus

2016-07-07

Two structure determination methods, based on the molecular dynamics flexible fitting (MDFF) paradigm, are presented that resolve sub-5 Å cryo-electron microscopy (EM) maps with either single structures or ensembles of such structures. The methods, denoted cascade MDFF and resolution exchange MDFF, sequentially re-refine a search model against a series of maps of progressively higher resolutions, which ends with the original experimental resolution. Application of sequential re-refinement enables MDFF to achieve a radius of convergence of ~25 Å demonstrated with the accurate modeling of β-galactosidase and TRPV1 proteins at 3.2 Å and 3.4 Å resolution, respectively. The MDFF refinements uniquely offer map-model validation and B-factor determination criteria based on the inherent dynamics of the macromolecules studied, captured by means of local root mean square fluctuations. The MDFF tools described are available to researchers through an easy-to-use and cost-effective cloud computing resource on Amazon Web Services.

Resolution limits of ultrafast ultrasound localization microscopy

NASA Astrophysics Data System (ADS)

Desailly, Yann; Pierre, Juliette; Couture, Olivier; Tanter, Mickael

2015-11-01

As in other imaging methods based on waves, the resolution of ultrasound imaging is limited by the wavelength. However, the diffraction-limit can be overcome by super-localizing single events from isolated sources. In recent years, we developed plane-wave ultrasound allowing frame rates up to 20 000 fps. Ultrafast processes such as rapid movement or disruption of ultrasound contrast agents (UCA) can thus be monitored, providing us with distinct punctual sources that could be localized beyond the diffraction limit. We previously showed experimentally that resolutions beyond λ/10 can be reached in ultrafast ultrasound localization microscopy (uULM) using a 128 transducer matrix in reception. Higher resolutions are theoretically achievable and the aim of this study is to predict the maximum resolution in uULM with respect to acquisition parameters (frequency, transducer geometry, sampling electronics). The accuracy of uULM is the error on the localization of a bubble, considered a point-source in a homogeneous medium. The proposed model consists in two steps: determining the timing accuracy of the microbubble echo in radiofrequency data, then transferring this time accuracy into spatial accuracy. The simplified model predicts a maximum resolution of 40 μm for a 1.75 MHz transducer matrix composed of two rows of 64 elements. Experimental confirmation of the model was performed by flowing microbubbles within a 60 μm microfluidic channel and localizing their blinking under ultrafast imaging (500 Hz frame rate). The experimental resolution, determined as the standard deviation in the positioning of the microbubbles, was predicted within 6 μm (13%) of the theoretical values and followed the analytical relationship with respect to the number of elements and depth. Understanding the underlying physical principles determining the resolution of superlocalization will allow the optimization of the imaging setup for each organ. Ultimately, accuracies better than the size of capillaries are achievable at several centimeter depths.

Applications of PET CT in clinical practice: Present and future

NASA Astrophysics Data System (ADS)

Costa, Durval Campos

2007-02-01

Radionuclide imaging and specially positron emission tomography (PET) has already demonstrated its benefits in three major medical subjects, i.e. neurology, cardiology and particularly clinical oncology. More recently the combination of PET and X-ray computed tomography (CT) as PET-CT led to a significant increment of the already large number of clinical applications of this imaging modality. This "anatomy-metabolic fusion" also known as Metabolic Imaging has its future assured if we can: (1) improve resolution reducing partial volume effect, (2) achieve very fast whole body imaging, (3) obtain accurate quantification of specific functions with higher contrast resolution and, if possible, (4) reduce exposure rates due to the unavoidable use of ionizing radiation.

A study of the effects of strong magnetic fields on the image resolution of PET scanners

NASA Astrophysics Data System (ADS)

Burdette, Don J.

Very high resolution images can be achieved in small animal PET systems utilizing solid state silicon pad detectors. In such systems using detectors with sub-millimeter intrinsic resolutions, the range of the positron is the largest contribution to the image blur. The size of the positron range effect depends on the initial positron energy and hence the radioactive tracer used. For higher energy positron emitters, such as 68Ga and 94mTc, the variation of the annihilation point dominates the spatial resolution. In this study two techniques are investigated to improve the image resolution of PET scanners limited by the range of the positron. One, the positron range can be reduced by embedding the PET field of view in a strong magnetic field. We have developed a silicon pad detector based PET instrument that can operate in strong magnetic fields with an image resolution of 0.7 mm FWHM to study this effect. Two, iterative reconstruction methods can be used to statistically correct for the range of the positron. Both strong magnetic fields and iterative reconstruction algorithms that statistically account for the positron range distribution are investigated in this work.

Improving PET spatial resolution and detectability for prostate cancer imaging

NASA Astrophysics Data System (ADS)

Bal, H.; Guerin, L.; Casey, M. E.; Conti, M.; Eriksson, L.; Michel, C.; Fanti, S.; Pettinato, C.; Adler, S.; Choyke, P.

2014-08-01

Prostate cancer, one of the most common forms of cancer among men, can benefit from recent improvements in positron emission tomography (PET) technology. In particular, better spatial resolution, lower noise and higher detectability of small lesions could be greatly beneficial for early diagnosis and could provide a strong support for guiding biopsy and surgery. In this article, the impact of improved PET instrumentation with superior spatial resolution and high sensitivity are discussed, together with the latest development in PET technology: resolution recovery and time-of-flight reconstruction. Using simulated cancer lesions, inserted in clinical PET images obtained with conventional protocols, we show that visual identification of the lesions and detectability via numerical observers can already be improved using state of the art PET reconstruction methods. This was achieved using both resolution recovery and time-of-flight reconstruction, and a high resolution image with 2 mm pixel size. Channelized Hotelling numerical observers showed an increase in the area under the LROC curve from 0.52 to 0.58. In addition, a relationship between the simulated input activity and the area under the LROC curve showed that the minimum detectable activity was reduced by more than 23%.

A Virtual Study of Grid Resolution on Experiments of a Highly-Resolved Turbulent Plume

NASA Astrophysics Data System (ADS)

Maisto, Pietro M. F.; Marshall, Andre W.; Gollner, Michael J.; Fire Protection Engineering Department Collaboration

2017-11-01

An accurate representation of sub-grid scale turbulent mixing is critical for modeling fire plumes and smoke transport. In this study, PLIF and PIV diagnostics are used with the saltwater modeling technique to provide highly-resolved instantaneous field measurements in unconfined turbulent plumes useful for statistical analysis, physical insight, and model validation. The effect of resolution was investigated employing a virtual interrogation window (of varying size) applied to the high-resolution field measurements. Motivated by LES low-pass filtering concepts, the high-resolution experimental data in this study can be analyzed within the interrogation windows (i.e. statistics at the sub-grid scale) and on interrogation windows (i.e. statistics at the resolved scale). A dimensionless resolution threshold (L/D*) criterion was determined to achieve converged statistics on the filtered measurements. Such a criterion was then used to establish the relative importance between large and small-scale turbulence phenomena while investigating specific scales for the turbulent flow. First order data sets start to collapse at a resolution of 0.3D*, while for second and higher order statistical moments the interrogation window size drops down to 0.2D*.

Hyperspectral imagery super-resolution by compressive sensing inspired dictionary learning and spatial-spectral regularization.

PubMed

Huang, Wei; Xiao, Liang; Liu, Hongyi; Wei, Zhihui

2015-01-19

Due to the instrumental and imaging optics limitations, it is difficult to acquire high spatial resolution hyperspectral imagery (HSI). Super-resolution (SR) imagery aims at inferring high quality images of a given scene from degraded versions of the same scene. This paper proposes a novel hyperspectral imagery super-resolution (HSI-SR) method via dictionary learning and spatial-spectral regularization. The main contributions of this paper are twofold. First, inspired by the compressive sensing (CS) framework, for learning the high resolution dictionary, we encourage stronger sparsity on image patches and promote smaller coherence between the learned dictionary and sensing matrix. Thus, a sparsity and incoherence restricted dictionary learning method is proposed to achieve higher efficiency sparse representation. Second, a variational regularization model combing a spatial sparsity regularization term and a new local spectral similarity preserving term is proposed to integrate the spectral and spatial-contextual information of the HSI. Experimental results show that the proposed method can effectively recover spatial information and better preserve spectral information. The high spatial resolution HSI reconstructed by the proposed method outperforms reconstructed results by other well-known methods in terms of both objective measurements and visual evaluation.

Comparison of multi-arm VRX CT scanners through computer models

NASA Astrophysics Data System (ADS)

Rendon, David A.; DiBianca, Frank A.; Keyes, Gary S.

2007-03-01

Variable Resolution X-ray (VRX) CT scanners allow imaging of different sized anatomy at the same level of detail using the same device. This is achieved by tilting the x-ray detectors so that the projected size of the detecting elements is varied producing reconstructions of smaller fields of view with higher spatial resolution.1 The detector can be divided in two or more separate segments, called arms, which can be placed at different angles, allowing some flexibility for the scanner design. In particular, several arms can be set at different angles creating a target region of considerably higher resolution that can be used to track the evolution of a previously diagnosed condition, while keeping the patient completely inside the field of view (FOV).2 This work presents newly-developed computer models of single-slice VRX scanners that allow us to study and compare different configurations (that is, various types of detectors arranged in any number of arms arranged in different geometries) in terms of spatial and contrast resolution. In particular, we are interested in comparing the performance of various geometric configurations that would otherwise be considered equivalent (using the same equipment, imaging FOVs of the same sizes, and having a similar overall scanner size). For this, a VRX simulator was developed, along with mathematical phantoms for spatial resolution and contrast analysis. These tools were used to compare scanner configurations that can be reproduced with materials presently available in our lab.

High-resolution whole-brain DCE-MRI using constrained reconstruction: Prospective clinical evaluation in brain tumor patients

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guo, Yi, E-mail: yiguo@usc.edu; Zhu, Yinghua; Lingala, Sajan Goud

Purpose: To clinically evaluate a highly accelerated T1-weighted dynamic contrast-enhanced (DCE) MRI technique that provides high spatial resolution and whole-brain coverage via undersampling and constrained reconstruction with multiple sparsity constraints. Methods: Conventional (rate-2 SENSE) and experimental DCE-MRI (rate-30) scans were performed 20 minutes apart in 15 brain tumor patients. The conventional clinical DCE-MRI had voxel dimensions 0.9 × 1.3 × 7.0 mm{sup 3}, FOV 22 × 22 × 4.2 cm{sup 3}, and the experimental DCE-MRI had voxel dimensions 0.9 × 0.9 × 1.9 mm{sup 3}, and broader coverage 22 × 22 × 19 cm{sup 3}. Temporal resolution was 5 smore » for both protocols. Time-resolved images and blood–brain barrier permeability maps were qualitatively evaluated by two radiologists. Results: The experimental DCE-MRI scans showed no loss of qualitative information in any of the cases, while achieving substantially higher spatial resolution and whole-brain spatial coverage. Average qualitative scores (from 0 to 3) were 2.1 for the experimental scans and 1.1 for the conventional clinical scans. Conclusions: The proposed DCE-MRI approach provides clinically superior image quality with higher spatial resolution and coverage than currently available approaches. These advantages may allow comprehensive permeability mapping in the brain, which is especially valuable in the setting of large lesions or multiple lesions spread throughout the brain.« less

Functional cardiac magnetic resonance microscopy

NASA Astrophysics Data System (ADS)

Brau, Anja Christina Sophie

2003-07-01

The study of small animal models of human cardiovascular disease is critical to our understanding of the origin, progression, and treatment of this pervasive disease. Complete analysis of disease pathophysiology in these animal models requires measuring structural and functional changes at the level of the whole heart---a task for which an appropriate non-invasive imaging method is needed. The purpose of this work was thus to develop an imaging technique to support in vivo characterization of cardiac structure and function in rat and mouse models of cardiovascular disease. Whereas clinical cardiac magnetic resonance imaging (MRI) provides accurate assessment of the human heart, the extension of cardiac MRI from humans to rodents presents several formidable scaling challenges. Acquiring images of the mouse heart with organ definition and fluidity of contraction comparable to that achieved in humans requires an increase in spatial resolution by a factor of 3000 and an increase in temporal resolution by a factor of ten. No single technical innovation can meet the demanding imaging requirements imposed by the small animal. A functional cardiac magnetic resonance microscopy technique was developed by integrating improvements in physiological control, imaging hardware, biological synchronization of imaging, and pulse sequence design to achieve high-quality images of the murine heart with high spatial and temporal resolution. The specific methods and results from three different sets of imaging experiments are presented: (1) 2D functional imaging in the rat with spatial resolution of 175 mum2 x 1 mm and temporal resolution of 10 ms; (2) 3D functional imaging in the rat with spatial resolution of 100 mum 2 x 500 mum and temporal resolution of 30 ms; and (3) 2D functional imaging in the mouse with spatial resolution down to 100 mum2 x 1 mm and temporal resolution of 10 ms. The cardiac microscopy technique presented here represents a novel collection of technologies capable of acquiring routine high-quality images of murine cardiac structure and function with minimal artifacts and markedly higher spatial resolution compared to conventional techniques. This work is poised to serve a valuable role in the evaluation of cardiovascular disease and should find broad application in studies ranging from basic pathophysiology to drug discovery.

High-Resolution Characterization of UMo Alloy Microstructure

DOE Office of Scientific and Technical Information (OSTI.GOV)

Devaraj, Arun; Kovarik, Libor; Joshi, Vineet V.

2016-11-30

This report highlights the capabilities and procedure for high-resolution characterization of UMo fuels in PNNL. Uranium-molybdenum (UMo) fuel processing steps, from casting to forming final fuel, directly affect the microstructure of the fuel, which in turn dictates the in-reactor performance of the fuel under irradiation. In order to understand the influence of processing on UMo microstructure, microstructure characterization techniques are necessary. Higher-resolution characterization techniques like transmission electron microscopy (TEM) and atom probe tomography (APT) are needed to interrogate the details of the microstructure. The findings from TEM and APT are also directly beneficial for developing predictive multiscale modeling tools thatmore » can predict the microstructure as a function of process parameters. This report provides background on focused-ion-beam–based TEM and APT sample preparation, TEM and APT analysis procedures, and the unique information achievable through such advanced characterization capabilities for UMo fuels, from a fuel fabrication capability viewpoint.« less

[Extraction of buildings three-dimensional information from high-resolution satellite imagery based on Barista software].

PubMed

Zhang, Pei-feng; Hu, Yuan-man; He, Hong-shi

2010-05-01

The demand for accurate and up-to-date spatial information of urban buildings is becoming more and more important for urban planning, environmental protection, and other vocations. Today's commercial high-resolution satellite imagery offers the potential to extract the three-dimensional information of urban buildings. This paper extracted the three-dimensional information of urban buildings from QuickBird imagery, and validated the precision of the extraction based on Barista software. It was shown that the extraction of three-dimensional information of the buildings from high-resolution satellite imagery based on Barista software had the advantages of low professional level demand, powerful universality, simple operation, and high precision. One pixel level of point positioning and height determination accuracy could be achieved if the digital elevation model (DEM) and sensor orientation model had higher precision and the off-Nadir View Angle was relatively perfect.

Endoscopic Full-Field Swept-Source Optical Coherence Tomography Neuroimaging System

NASA Astrophysics Data System (ADS)

Felts Almog, Ilan

Optical Coherence Tomography (OCT) has the capability to differentiate brain elements with intrinsic contrast and at a resolution an order-of-magnitude higher than other imaging modalities. This thesis investigates the feasibility of OCT for neuroimaging applied to neurosurgical guidance. We present, to our knowledge, the first Full-Field Swept-Source OCT system operating near a wavelength of 1310 nm, achieving a transverse imaging resolution of 6.5 mum, an axial resolution of 14 mum in tissue and a field of view of 270 mum x 180 mum x 400 mum. Imaging experiments were performed on rat brain tissues ex vivo, human cortical tissue ex vivo, and rats in vivo. A multi-level threshold metric applied on the intensity of the images led to a plausible correlation between the observed density and location in the brain. The proof-of-concept OCT system can be improved and miniaturized for clinical use.

Precise and fast spatial-frequency analysis using the iterative local Fourier transform.

PubMed

Lee, Sukmock; Choi, Heejoo; Kim, Dae Wook

2016-09-19

The use of the discrete Fourier transform has decreased since the introduction of the fast Fourier transform (fFT), which is a numerically efficient computing process. This paper presents the iterative local Fourier transform (ilFT), a set of new processing algorithms that iteratively apply the discrete Fourier transform within a local and optimal frequency domain. The new technique achieves 2¹⁰ times higher frequency resolution than the fFT within a comparable computation time. The method's superb computing efficiency, high resolution, spectrum zoom-in capability, and overall performance are evaluated and compared to other advanced high-resolution Fourier transform techniques, such as the fFT combined with several fitting methods. The effectiveness of the ilFT is demonstrated through the data analysis of a set of Talbot self-images (1280 × 1024 pixels) obtained with an experimental setup using grating in a diverging beam produced by a coherent point source.

Performance analysis of multiple PRF technique for ambiguity resolution

NASA Technical Reports Server (NTRS)

Chang, C. Y.; Curlander, J. C.

1992-01-01

For short wavelength spaceborne synthetic aperture radar (SAR), ambiguity in Doppler centroid estimation occurs when the azimuth squint angle uncertainty is larger than the azimuth antenna beamwidth. Multiple pulse recurrence frequency (PRF) hopping is a technique developed to resolve the ambiguity by operating the radar in different PRF's in the pre-imaging sequence. Performance analysis results of the multiple PRF technique are presented, given the constraints of the attitude bound, the drift rate uncertainty, and the arbitrary numerical values of PRF's. The algorithm performance is derived in terms of the probability of correct ambiguity resolution. Examples, using the Shuttle Imaging Radar-C (SIR-C) and X-SAR parameters, demonstrate that the probability of correct ambiguity resolution obtained by the multiple PRF technique is greater than 95 percent and 80 percent for the SIR-C and X-SAR applications, respectively. The success rate is significantly higher than that achieved by the range cross correlation technique.

Laser Beam Filtration for High Spatial Resolution MALDI Imaging Mass Spectrometry

NASA Astrophysics Data System (ADS)

Zavalin, Andre; Yang, Junhai; Caprioli, Richard

2013-07-01

We describe an easy and inexpensive way to provide a highly defined Gaussian shaped laser spot on target of 5 μm diameter for imaging mass spectrometry using a commercial MALDI TOF instrument that is designed to produce a 20 μm diameter laser beam on target at its lowest setting. A 25 μm pinhole filter on a swivel arm was installed in the laser beam optics outside the vacuum ion source chamber so it is easily flipped into or out of the beam as desired by the operator. The resulting ion images at 5 μm spatial resolution are sharp since the satellite secondary laser beam maxima have been removed by the filter. Ion images are shown to demonstrate the performance and are compared with the method of oversampling to achieve higher spatial resolution when only a larger laser beam spot on target is available.

Investigating the influence of chromatic aberration and optical illumination bandwidth on fundus imaging in rats

NASA Astrophysics Data System (ADS)

Li, Hao; Liu, Wenzhong; Zhang, Hao F.

2015-10-01

Rodent models are indispensable in studying various retinal diseases. Noninvasive, high-resolution retinal imaging of rodent models is highly desired for longitudinally investigating the pathogenesis and therapeutic strategies. However, due to severe aberrations, the retinal image quality in rodents can be much worse than that in humans. We numerically and experimentally investigated the influence of chromatic aberration and optical illumination bandwidth on retinal imaging. We confirmed that the rat retinal image quality decreased with increasing illumination bandwidth. We achieved the retinal image resolution of 10 μm using a 19 nm illumination bandwidth centered at 580 nm in a home-built fundus camera. Furthermore, we observed higher chromatic aberration in albino rat eyes than in pigmented rat eyes. This study provides a design guide for high-resolution fundus camera for rodents. Our method is also beneficial to dispersion compensation in multiwavelength retinal imaging applications.

Silicone Molding and Lifetime Testing of Peripheral Nerve Interfaces for Neuroprostheses

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gupte, Kimaya; Tolosa, Vanessa

Implantable peripheral nerve cuffs have a large application in neuroprostheses as they can be used to restore sensation to those with upper limb amputations. Modern day prosthetics, while lessening the pain associated with phantom limb syndrome, have limited fine motor control and do not provide sensory feedback to patients. Sensory feedback with prosthetics requires communication between the nervous system and limbs, and is still a challenge to accomplish with amputees. Establishing this communication between the peripheral nerves in the arm and artificial limbs is vital as prosthetics research aims to provide sensory feedback to amputees. Peripheral nerve cuffs restore sensationmore » by electrically stimulating certain parts of the nerve in order to create feeling in the hand. Cuff electrodes have an advantage over standard electrodes as they have high selective stimulation by bringing the electrical interface close to the neural tissue in order to selectively activate targeted regions of a peripheral nerve. In order to further improve the selective stimulation of these nerve cuffs, there is need for finer spatial resolution among electrodes. One method to achieve a higher spatial resolution is to increase the electrode density on the cuff itself. Microfabrication techniques can be used to achieve this higher electrode density. Using L-Edit, a layout editor, microfabricated peripheral nerve cuffs were designed with a higher electrode density than the current model. This increase in electrode density translates to an increase in spatial resolution by at least one order of magnitude. Microfabricated devices also have two separate components that are necessary to understand before implantation: lifetime of the device and assembly to prevent nerve damage. Silicone molding procedures were optimized so that devices do not damage nerves in vivo, and lifetime testing was performed on test microfabricated devices to determine their lifetime in vivo. Future work of this project would include fabricating some of the designed devices and seeing how they compare to the current cuffs in terms of their electrical performance, lifetime, shape, and mechanical properties.« less

JPRS Report, Soviet Union: Political Affairs

DTIC Science & Technology

1988-05-24

Azerbaijani Komsomol Resolution on Increasing Interaction [Editorial; KOMSOMOLETS 7 Apr 88] 47 Appeal to Armenian Komsomol Members for Discipline, Public...area, and certain of them, as V. S. Semenov recently said in VOPROSY FILOSOFII, "once again the ’first’ are already appealing to us to show boldness...and even of almost uncon- cealed appeals to higher administrative echelons to achieve the "brainwashing" of one’s opponents. The methods are still

Detecting personnel around UGVs using stereo vision

NASA Astrophysics Data System (ADS)

Bajracharya, Max; Moghaddam, Baback; Howard, Andrew; Matthies, Larry H.

2008-04-01

Detecting people around unmanned ground vehicles (UGVs) to facilitate safe operation of UGVs is one of the highest priority issues in the development of perception technology for autonomous navigation. Research to date has not achieved the detection ranges or reliability needed in deployed systems to detect upright pedestrians in flat, relatively uncluttered terrain, let alone in more complex environments and with people in postures that are more difficult to detect. Range data is essential to solve this problem. Combining range data with high resolution imagery may enable higher performance than range data alone because image appearance can complement shape information in range data and because cameras may offer higher angular resolution than typical range sensors. This makes stereo vision a promising approach for several reasons: image resolution is high and will continue to increase, the physical size and power dissipation of the cameras and computers will continue to decrease, and stereo cameras provide range data and imagery that are automatically spatially and temporally registered. We describe a stereo vision-based pedestrian detection system, focusing on recent improvements to a shape-based classifier applied to the range data, and present frame-level performance results that show great promise for the overall approach.

Super-Resolution Imaging Strategies for Cell Biologists Using a Spinning Disk Microscope

PubMed Central

Hosny, Neveen A.; Song, Mingying; Connelly, John T.; Ameer-Beg, Simon; Knight, Martin M.; Wheeler, Ann P.

2013-01-01

In this study we use a spinning disk confocal microscope (SD) to generate super-resolution images of multiple cellular features from any plane in the cell. We obtain super-resolution images by using stochastic intensity fluctuations of biological probes, combining Photoactivation Light-Microscopy (PALM)/Stochastic Optical Reconstruction Microscopy (STORM) methodologies. We compared different image analysis algorithms for processing super-resolution data to identify the most suitable for analysis of particular cell structures. SOFI was chosen for X and Y and was able to achieve a resolution of ca. 80 nm; however higher resolution was possible >30 nm, dependant on the super-resolution image analysis algorithm used. Our method uses low laser power and fluorescent probes which are available either commercially or through the scientific community, and therefore it is gentle enough for biological imaging. Through comparative studies with structured illumination microscopy (SIM) and widefield epifluorescence imaging we identified that our methodology was advantageous for imaging cellular structures which are not immediately at the cell-substrate interface, which include the nuclear architecture and mitochondria. We have shown that it was possible to obtain two coloured images, which highlights the potential this technique has for high-content screening, imaging of multiple epitopes and live cell imaging. PMID:24130668

Magnetic resonance imaging of the inner ear by using a hybrid radiofrequency coil at 7 T

NASA Astrophysics Data System (ADS)

Kim, Kyoung-Nam; Heo, Phil; Kim, Young-Bo; Han, Gyu-Cheol

2015-01-01

Visualization of the membranous structures of the inner ear has been limited to the detection of the normal fluid signal intensity within the bony labyrinth by using magnetic resonance imaging (MRI) equipped with a 1.5 Tesla (T) magnet. High-field (HF) MRI has been available for more than a decade, and numerous studies have documented its significant advantages over conventional MRI with regards to its use in basic scientific research and routine clinical assessments. No previous studies of the inner ear by using HF MRI have been reported, in part because high-quality resolution of mastoid pneumatization is challenging due to artifacts generated in the HF environment and insufficient performance of radiofrequency (RF) coils. Therefore, a hybrid RF coil with integrated circuitry was developed at 7 T and was targeted for anatomical imaging to achieve a high resolution image of the structure of the human inner ear, excluding the bony portion. The inner-ear's structure is composed of soft tissues containing hydrogen ions and includes the membranous labyrinth, endolymphatic space, perilymphatic space, and cochlear-vestibular nerves. Visualization of the inner-ear's anatomy was performed in-vivo with a custom-designed hybrid RF coil and a specific imaging protocol based on an interpolated breath-held examination sequence. The comparative signal intensity value at 30-mm away from the phantom side was 88% higher for the hybrid RF coil and 24% higher for the 8-channel transmit/receive (Tx/Rx) coil than for the commercial birdcage coil. The optimized MRI protocol employed a hybrid RF coil because it enabled high-resolution imaging of the inner-ear's anatomy and accurate mapping of structures including the cochlea and the semicircular canals. These results indicate that 7 T MRI achieves high spatial resolution visualization of the inner-ear's anatomy. Therefore, MRI imaging using a hybrid RF coil at 7 T could provide a powerful tool for clinical investigations of petrous pathologies of the inner ear.

40 MHz high-frequency ultrafast ultrasound imaging.

PubMed

Huang, Chih-Chung; Chen, Pei-Yu; Peng, Po-Hsun; Lee, Po-Yang

2017-06-01

Ultrafast high-frame-rate ultrasound imaging based on coherent-plane-wave compounding has been developed for many biomedical applications. Most coherent-plane-wave compounding systems typically operate at 3-15 MHz, and the image resolution for this frequency range is not sufficient for visualizing microstructure tissues. Therefore, the purpose of this study was to implement a high-frequency ultrafast ultrasound imaging operating at 40 MHz. The plane-wave compounding imaging and conventional multifocus B-mode imaging were performed using the Field II toolbox of MATLAB in simulation study. In experiments, plane-wave compounding images were obtained from a 256 channel ultrasound research platform with a 40 MHz array transducer. All images were produced by point-spread functions and cyst phantoms. The in vivo experiment was performed from zebrafish. Since high-frequency ultrasound exhibits a lower penetration, chirp excitation was applied to increase the imaging depth in simulation. The simulation results showed that a lateral resolution of up to 66.93 μm and a contrast of up to 56.41 dB were achieved when using 75-angles plane waves in compounding imaging. The experimental results showed that a lateral resolution of up to 74.83 μm and a contrast of up to 44.62 dB were achieved when using 75-angles plane waves in compounding imaging. The dead zone and compounding noise are about 1.2 mm and 2.0 mm in depth for experimental compounding imaging, respectively. The structure of zebrafish heart was observed clearly using plane-wave compounding imaging. The use of fewer than 23 angles for compounding allowed a frame rate higher than 1000 frames per second. However, the compounding imaging exhibits a similar lateral resolution of about 72 μm as the angle of plane wave is higher than 10 angles. This study shows the highest operational frequency for ultrafast high-frame-rate ultrasound imaging. © 2017 American Association of Physicists in Medicine.

Developing a bright 17 keV x-ray source for probing high-energy-density states of matter at high spatial resolution

NASA Astrophysics Data System (ADS)

Huntington, C. M.; Park, H.-S.; Maddox, B. R.; Barrios, M. A.; Benedetti, R.; Braun, D. G.; Hohenberger, M.; Landen, O. L.; Regan, S. P.; Wehrenberg, C. E.; Remington, B. A.

2015-04-01

A set of experiments were performed on the National Ignition Facility (NIF) to develop and optimize a bright, 17 keV x-ray backlighter probe using laser-irradiated Nb foils. High-resolution one-dimensional imaging was achieved using a 15 μm wide slit in a Ta substrate to aperture the Nb Heα x-rays onto an open-aperture, time integrated camera. To optimize the x-ray source for imaging applications, the effect of laser pulse shape and spatial profile on the target was investigated. Two laser pulse shapes were used—a "prepulse" shape that included a 3 ns, low-intensity laser foot preceding the high-energy 2 ns square main laser drive, and a pulse without the laser foot. The laser spatial profile was varied by the use of continuous phase plates (CPPs) on a pair of shots compared to beams at best focus, without CPPs. A comprehensive set of common diagnostics allowed for a direct comparison of imaging resolution, total x-ray conversion efficiency, and x-ray spectrum between shots. The use of CPPs was seen to reduce the high-energy tail of the x-ray spectrum, whereas the laser pulse shape had little effect on the high-energy tail. The measured imaging resolution was comparably high for all combinations of laser parameters, but a higher x-ray flux was achieved without phase plates. This increased flux was the result of smaller laser spot sizes, which allowed us to arrange the laser focal spots from multiple beams and produce an x-ray source which was more localized behind the slit aperture. Our experiments are a first demonstration of point-projection geometry imaging at NIF at the energies (>10 keV) necessary for imaging denser, higher-Z targets than have previously been investigated.

Image reconstructions from super-sampled data sets with resolution modeling in PET imaging.

PubMed

Li, Yusheng; Matej, Samuel; Metzler, Scott D

2014-12-01

Spatial resolution in positron emission tomography (PET) is still a limiting factor in many imaging applications. To improve the spatial resolution for an existing scanner with fixed crystal sizes, mechanical movements such as scanner wobbling and object shifting have been considered for PET systems. Multiple acquisitions from different positions can provide complementary information and increased spatial sampling. The objective of this paper is to explore an efficient and useful reconstruction framework to reconstruct super-resolution images from super-sampled low-resolution data sets. The authors introduce a super-sampling data acquisition model based on the physical processes with tomographic, downsampling, and shifting matrices as its building blocks. Based on the model, we extend the MLEM and Landweber algorithms to reconstruct images from super-sampled data sets. The authors also derive a backprojection-filtration-like (BPF-like) method for the super-sampling reconstruction. Furthermore, they explore variant methods for super-sampling reconstructions: the separate super-sampling resolution-modeling reconstruction and the reconstruction without downsampling to further improve image quality at the cost of more computation. The authors use simulated reconstruction of a resolution phantom to evaluate the three types of algorithms with different super-samplings at different count levels. Contrast recovery coefficient (CRC) versus background variability, as an image-quality metric, is calculated at each iteration for all reconstructions. The authors observe that all three algorithms can significantly and consistently achieve increased CRCs at fixed background variability and reduce background artifacts with super-sampled data sets at the same count levels. For the same super-sampled data sets, the MLEM method achieves better image quality than the Landweber method, which in turn achieves better image quality than the BPF-like method. The authors also demonstrate that the reconstructions from super-sampled data sets using a fine system matrix yield improved image quality compared to the reconstructions using a coarse system matrix. Super-sampling reconstructions with different count levels showed that the more spatial-resolution improvement can be obtained with higher count at a larger iteration number. The authors developed a super-sampling reconstruction framework that can reconstruct super-resolution images using the super-sampling data sets simultaneously with known acquisition motion. The super-sampling PET acquisition using the proposed algorithms provides an effective and economic way to improve image quality for PET imaging, which has an important implication in preclinical and clinical region-of-interest PET imaging applications.

High-resolution whole-brain diffusion MRI at 7T using radiofrequency parallel transmission.

PubMed

Wu, Xiaoping; Auerbach, Edward J; Vu, An T; Moeller, Steen; Lenglet, Christophe; Schmitter, Sebastian; Van de Moortele, Pierre-François; Yacoub, Essa; Uğurbil, Kâmil

2018-03-30

Investigating the utility of RF parallel transmission (pTx) for Human Connectome Project (HCP)-style whole-brain diffusion MRI (dMRI) data at 7 Tesla (7T). Healthy subjects were scanned in pTx and single-transmit (1Tx) modes. Multiband (MB), single-spoke pTx pulses were designed to image sagittal slices. HCP-style dMRI data (i.e., 1.05-mm resolutions, MB2, b-values = 1000/2000 s/mm 2 , 286 images and 40-min scan) and data with higher accelerations (MB3 and MB4) were acquired with pTx. pTx significantly improved flip-angle detected signal uniformity across the brain, yielding ∼19% increase in temporal SNR (tSNR) averaged over the brain relative to 1Tx. This allowed significantly enhanced estimation of multiple fiber orientations (with ∼21% decrease in dispersion) in HCP-style 7T dMRI datasets. Additionally, pTx pulses achieved substantially lower power deposition, permitting higher accelerations, enabling collection of the same data in 2/3 and 1/2 the scan time or of more data in the same scan time. pTx provides a solution to two major limitations for slice-accelerated high-resolution whole-brain dMRI at 7T; it improves flip-angle uniformity, and enables higher slice acceleration relative to current state-of-the-art. As such, pTx provides significant advantages for rapid acquisition of high-quality, high-resolution truly whole-brain dMRI data. © 2018 International Society for Magnetic Resonance in Medicine.

A Broadband X-Ray Imaging Spectroscopy with High-Angular Resolution: the FORCE Mission

NASA Technical Reports Server (NTRS)

Mori, Koji; Tsuru, Takeshi Go; Nakazawac, Kazuhiro; Ueda, Yoshihiro; Okajima, Takashi; Murakami, Hiroshi; Awaki, Hisamitsu; Matsumoto, Hironori; Fukazawai, Yasushi; Tsunemi, Hiroshi;

A broadband x-ray imaging spectroscopy with high-angular resolution: the FORCE mission

NASA Astrophysics Data System (ADS)

Mori, Koji; Tsuru, Takeshi Go; Nakazawa, Kazuhiro; Ueda, Yoshihiro; Okajima, Takashi; Murakami, Hiroshi; Awaki, Hisamitsu; Matsumoto, Hironori; Fukazawa, Yasushi; Tsunemi, Hiroshi; Takahashi, Tadayuki; Zhang, William W.

2016-07-01

We are proposing FORCE (Focusing On Relativistic universe and Cosmic Evolution) as a future Japan-lead Xray observatory to be launched in the mid 2020s. Hitomi (ASTRO-H) possesses a suite of sensitive instruments enabling the highest energy-resolution spectroscopy in soft X-ray band, a broadband X-ray imaging spectroscopy in soft and hard X-ray bands, and further high energy coverage up to soft gamma-ray band. FORCE is the direct successor to the broadband X-ray imaging spectroscopy aspect of Hitomi (ASTRO-H) with significantly higher angular resolution. The current design of FORCE defines energy band pass of 1-80 keV with angular resolution of < 15 in half-power diameter, achieving a 10 times higher sensitivity above 10 keV compared to any previous missions with simultaneous soft X-ray coverage. Our primary scientific objective is to trace the cosmic formation history by searching for "missing black holes" in various mass-scales: "buried supermassive black holes (SMBHs)" (> 104 M⊙) residing in the center of galaxies in a cosmological distance, "intermediate-mass black holes" (102-104 M⊙) acting as the possible seeds from which SMBHs grow, and "orphan stellar-mass black holes" (< 102 M⊙) without companion in our Galaxy. In addition to these missing BHs, hunting for the nature of relativistic particles at various astrophysical shocks is also in our scope, utilizing the broadband X-ray coverage with high angular-resolution. FORCE are going to open a new era in these fields. The satellite is proposed to be launched with the Epsilon vehicle that is a Japanese current solid-fuel rocket. FORCE carries three identical pairs of Super-mirror and wide-band X-ray detector. The focal length is currently planned to be 10 m. The silicon mirror with multi-layer coating is our primary choice to achieve lightweight, good angular optics. The detector is a descendant of hard X-ray imager onboard Hitomi (ASTRO-H) replacing its silicon strip detector with SOI-CMOS silicon pixel detector, allowing an extension of the low energy threshold down to 1 keV or even less.

Prospects for higher spatial resolution quantitative X-ray analysis using transition element L-lines

NASA Astrophysics Data System (ADS)

Statham, P.; Holland, J.

2014-03-01

Lowering electron beam kV reduces electron scattering and improves spatial resolution of X-ray analysis. However, a previous round robin analysis of steels at 5 - 6 kV using Lα-lines for the first row transition elements gave poor accuracies. Our experiments on SS63 steel using Lα-lines show similar biases in Cr and Ni that cannot be corrected with changes to self-absorption coefficients or carbon coating. The inaccuracy may be caused by different probabilities for emission and anomalous self-absorption for the La-line between specimen and pure element standard. Analysis using Ll(L3-M1)-lines gives more accurate results for SS63 plausibly because the M1-shell is not so vulnerable to the atomic environment as the unfilled M4,5-shell. However, Ll-intensities are very weak and WDS analysis may be impractical for some applications. EDS with large area SDD offers orders of magnitude faster analysis and achieves similar results to WDS analysis with Lα-lines but poorer energy resolution precludes the use of Ll-lines in most situations. EDS analysis of K-lines at low overvoltage is an alternative strategy for improving spatial resolution that could give higher accuracy. The trade-off between low kV versus low overvoltage is explored in terms of sensitivity for element detection for different elements.

Multicontrast reconstruction using compressed sensing with low rank and spatially varying edge-preserving constraints for high-resolution MR characterization of myocardial infarction.

PubMed

Zhang, Li; Athavale, Prashant; Pop, Mihaela; Wright, Graham A

2017-08-01

To enable robust reconstruction for highly accelerated three-dimensional multicontrast late enhancement imaging to provide improved MR characterization of myocardial infarction with isotropic high spatial resolution. A new method using compressed sensing with low rank and spatially varying edge-preserving constraints (CS-LASER) is proposed to improve the reconstruction of fine image details from highly undersampled data. CS-LASER leverages the low rank feature of the multicontrast volume series in MR relaxation and integrates spatially varying edge preservation into the explicit low rank constrained compressed sensing framework using weighted total variation. With an orthogonal temporal basis pre-estimated, a multiscale iterative reconstruction framework is proposed to enable the practice of CS-LASER with spatially varying weights of appropriate accuracy. In in vivo pig studies with both retrospective and prospective undersamplings, CS-LASER preserved fine image details better and presented tissue characteristics with a higher degree of consistency with histopathology, particularly in the peri-infarct region, than an alternative technique for different acceleration rates. An isotropic resolution of 1.5 mm was achieved in vivo within a single breath-hold using the proposed techniques. Accelerated three-dimensional multicontrast late enhancement with CS-LASER can achieve improved MR characterization of myocardial infarction with high spatial resolution. Magn Reson Med 78:598-610, 2017. © 2016 International Society for Magnetic Resonance in Medicine. © 2016 International Society for Magnetic Resonance in Medicine.

Mapping atomic motions with ultrabright electrons: towards fundamental limits in space-time resolution.

PubMed

Manz, Stephanie; Casandruc, Albert; Zhang, Dongfang; Zhong, Yinpeng; Loch, Rolf A; Marx, Alexander; Hasegawa, Taisuke; Liu, Lai Chung; Bayesteh, Shima; Delsim-Hashemi, Hossein; Hoffmann, Matthias; Felber, Matthias; Hachmann, Max; Mayet, Frank; Hirscht, Julian; Keskin, Sercan; Hada, Masaki; Epp, Sascha W; Flöttmann, Klaus; Miller, R J Dwayne

2015-01-01

The long held objective of directly observing atomic motions during the defining moments of chemistry has been achieved based on ultrabright electron sources that have given rise to a new field of atomically resolved structural dynamics. This class of experiments requires not only simultaneous sub-atomic spatial resolution with temporal resolution on the 100 femtosecond time scale but also has brightness requirements approaching single shot atomic resolution conditions. The brightness condition is in recognition that chemistry leads generally to irreversible changes in structure during the experimental conditions and that the nanoscale thin samples needed for electron structural probes pose upper limits to the available sample or "film" for atomic movies. Even in the case of reversible systems, the degree of excitation and thermal effects require the brightest sources possible for a given space-time resolution to observe the structural changes above background. Further progress in the field, particularly to the study of biological systems and solution reaction chemistry, requires increased brightness and spatial coherence, as well as an ability to tune the electron scattering cross-section to meet sample constraints. The electron bunch density or intensity depends directly on the magnitude of the extraction field for photoemitted electron sources and electron energy distribution in the transverse and longitudinal planes of electron propagation. This work examines the fundamental limits to optimizing these parameters based on relativistic electron sources using re-bunching cavity concepts that are now capable of achieving 10 femtosecond time scale resolution to capture the fastest nuclear motions. This analysis is given for both diffraction and real space imaging of structural dynamics in which there are several orders of magnitude higher space-time resolution with diffraction methods. The first experimental results from the Relativistic Electron Gun for Atomic Exploration (REGAE) are given that show the significantly reduced multiple electron scattering problem in this regime, which opens up micron scale systems, notably solution phase chemistry, to atomically resolved structural dynamics.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wu, Pei-Hsin; Chung, Hsiao-Wen; Tsai, Ping-Huei

Purpose: One of the technical advantages of functional magnetic resonance imaging (fMRI) is its precise localization of changes from neuronal activities. While current practice of fMRI acquisition at voxel size around 3 × 3 × 3 mm{sup 3} achieves satisfactory results in studies of basic brain functions, higher spatial resolution is required in order to resolve finer cortical structures. This study investigated spatial resolution effects on brain fMRI experiments using balanced steady-state free precession (bSSFP) imaging with 0.37 mm{sup 3} voxel volume at 3.0 T. Methods: In fMRI experiments, full and unilateral visual field 5 Hz flashing checkerboard stimulations weremore » given to healthy subjects. The bSSFP imaging experiments were performed at three different frequency offsets to widen the coverage, with functional activations in the primary visual cortex analyzed using the general linear model. Variations of the spatial resolution were achieved by removing outerk-space data components. Results: Results show that a reduction in voxel volume from 3.44 × 3.44 × 2 mm{sup 3} to 0.43 × 0.43 × 2 mm{sup 3} has resulted in an increase of the functional activation signals from (7.7 ± 1.7)% to (20.9 ± 2.0)% at 3.0 T, despite of the threefold SNR decreases in the original images, leading to nearly invariant functional contrast-to-noise ratios (fCNR) even at high spatial resolution. Activation signals aligning nicely with gray matter sulci at high spatial resolution would, on the other hand, have possibly been mistaken as noise at low spatial resolution. Conclusions: It is concluded that the bSSFP sequence is a plausible technique for fMRI investigations at submillimeter voxel widths without compromising fCNR. The reduction of partial volume averaging with nonactivated brain tissues to retain fCNR is uniquely suitable for high spatial resolution applications such as the resolving of columnar organization in the brain.« less

Estimation of position resolution for DOI-PET detector using diameter 0.2 mm WLS fibers [ANIMMA--2015-IO-x5

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kaneko, Naomi; Ito, H.; Han, S.

We have been developing a submillimeter resolution and low-cost DOI-PET detector using wavelength shifting fibers (WLSF), scintillating crystal plates and MPPCs (Hamamatsu Photonics). Conventional design of DOI-PET detectors had approximately mm{sup 3} of resolution by using some scintillating blocks with a volume of 1 mm{sup 3}, which detects gamma-ray. They are expensive due to difficulties in processing scintillating crystals and a large number of photo-detectors, and these technologies are likely to reach the limit of the resolution. Development of a lower cost DOI-PET detector with higher resolution is challenging to popularize the PET diagnosis. We propose two type of PETmore » detector. One is a whole body PET system, and the other is a PET system for brain or small animals. Each PET system consists 6 blocks. The former consists of 6 layers of crystal plates with 300 mm x 300 mm x 4 mm. The latter consists of 16 crystal layers, forming 4 x 4 crystal arrays. The size of the crystal plate is 40 mm x 40 mm x 1 mm. Wavelength shifting fiber (WLSF) sheets are attached to above and up and down side of crystal planes. The whole PET system has 8 MPPCs attached on each side. For the brain PET detector, 9 WLSF fibers are attached on the each side. The expected position resolution would be less than 1 mm at the former system. We have performed an experimental performance estimation for the system component using {sup 22}Na radioactive source. We achieved a collection efficiency of 10% using the WLSF sheet and Ce:Gd{sub 3}(Al,Ga){sub 5}O{sub 12} (GAGG) crystals at 511 keV. The linear relationship between reconstruction position and incident position was obtained, and a resolution of 0.7 mm (FWHM) for x-axis of DOI by the WLSF readout was achieved. (authors)« less

UWB Tracking Algorithms: AOA and TDOA

NASA Technical Reports Server (NTRS)

Ni, Jianjun David; Arndt, D.; Ngo, P.; Gross, J.; Refford, Melinda

2006-01-01

Ultra-Wideband (UWB) tracking prototype systems are currently under development at NASA Johnson Space Center for various applications on space exploration. For long range applications, a two-cluster Angle of Arrival (AOA) tracking method is employed for implementation of the tracking system; for close-in applications, a Time Difference of Arrival (TDOA) positioning methodology is exploited. Both AOA and TDOA are chosen to utilize the achievable fine time resolution of UWB signals. This talk presents a brief introduction to AOA and TDOA methodologies. The theoretical analysis of these two algorithms reveal the affecting parameters impact on the tracking resolution. For the AOA algorithm, simulations show that a tracking resolution less than 0.5% of the range can be achieved with the current achievable time resolution of UWB signals. For the TDOA algorithm used in close-in applications, simulations show that the (sub-inch) high tracking resolution is achieved with a chosen tracking baseline configuration. The analytical and simulated results provide insightful guidance for the UWB tracking system design.

Implementation of a pulse coupled neural network in FPGA.

PubMed

Waldemark, J; Millberg, M; Lindblad, T; Waldemark, K; Becanovic, V

2000-06-01

The Pulse Coupled neural network, PCNN, is a biologically inspired neural net and it can be used in various image analysis applications, e.g. time-critical applications in the field of image pre-processing like segmentation, filtering, etc. a VHDL implementation of the PCNN targeting FPGA was undertaken and the results presented here. The implementation contains many interesting features. By pipelining the PCNN structure a very high throughput of 55 million neuron iterations per second could be achieved. By making the coefficients re-configurable during operation, a complete recognition system could be implemented on one, or maybe two, chip(s). Reconsidering the ranges and resolutions of the constants may save a lot of hardware, since the higher resolution requires larger multipliers, adders, memories etc.

Analysis and an image recovery algorithm for ultrasonic tomography system

NASA Technical Reports Server (NTRS)

Jin, Michael Y.

1994-01-01

The problem of an ultrasonic reflectivity tomography is similar to that of a spotlight-mode aircraft Synthetic Aperture Radar (SAR) system. The analysis for a circular path spotlight mode SAR in this paper leads to the insight of the system characteristics. It indicates that such a system when operated in a wide bandwidth is capable of achieving the ultimate resolution; one quarter of the wavelength of the carrier frequency. An efficient processing algorithm based on the exact two dimensional spectrum is presented. The results of simulation indicate that the impulse responses meet the predicted resolution performance. Compared to an algorithm previously developed for the ultrasonic reflectivity tomography, the throughput rate of this algorithm is about ten times higher.

Design and development of a fast ion mass spectrometer

NASA Technical Reports Server (NTRS)

Burch, J. L.

1983-01-01

Two Fast Ion Mass Spectrometers (FIMS A and FIMS B) were developed. The design, development, construction, calibration, integration, and flight of these instruments, along with early results from the data analysis efforts are summarized. A medium energy ion mass spectrometer that covers mass velocity space with significantly higher time resolution, improved mass resolution, (particularly for heavier ions), and wider energy range than existing instruments had achieved was completed. The initial design consisted of a dual channel cylindrical electrostatic analyzer followed by a dual channel cylindrical velocity filter. The gain versus count rate characteristics of the high current channel electron multipliers (CEM's), which were chosen for ion detection, revealed a systematic behavior that can be used as a criterion for selection of CEM's for long counting lifetimes.

High resolution global flood hazard map from physically-based hydrologic and hydraulic models.

NASA Astrophysics Data System (ADS)

Begnudelli, L.; Kaheil, Y.; McCollum, J.

2017-12-01

The global flood map published online at http://www.fmglobal.com/research-and-resources/global-flood-map at 90m resolution is being used worldwide to understand flood risk exposure, exercise certain measures of mitigation, and/or transfer the residual risk financially through flood insurance programs. The modeling system is based on a physically-based hydrologic model to simulate river discharges, and 2D shallow-water hydrodynamic model to simulate inundation. The model can be applied to large-scale flood hazard mapping thanks to several solutions that maximize its efficiency and the use of parallel computing. The hydrologic component of the modeling system is the Hillslope River Routing (HRR) hydrologic model. HRR simulates hydrological processes using a Green-Ampt parameterization, and is calibrated against observed discharge data from several publicly-available datasets. For inundation mapping, we use a 2D Finite-Volume Shallow-Water model with wetting/drying. We introduce here a grid Up-Scaling Technique (UST) for hydraulic modeling to perform simulations at higher resolution at global scale with relatively short computational times. A 30m SRTM is now available worldwide along with higher accuracy and/or resolution local Digital Elevation Models (DEMs) in many countries and regions. UST consists of aggregating computational cells, thus forming a coarser grid, while retaining the topographic information from the original full-resolution mesh. The full-resolution topography is used for building relationships between volume and free surface elevation inside cells and computing inter-cell fluxes. This approach almost achieves computational speed typical of the coarse grids while preserving, to a significant extent, the accuracy offered by the much higher resolution available DEM. The simulations are carried out along each river of the network by forcing the hydraulic model with the streamflow hydrographs generated by HRR. Hydrographs are scaled so that the peak corresponds to the return period corresponding to the hazard map being produced (e.g. 100 years, 500 years). Each numerical simulation models one river reach, except for the longest reaches which are split in smaller parts. Here we show results for selected river basins worldwide.

Toward achieving precision health

PubMed Central

Gambhir, Sanjiv Sam; Ge, T. Jessie; Vermesh, Ophir; Spitler, Ryan

2018-01-01

Health care systems primarily focus on patients after they present with disease, not before. The emerging field of precision health encourages disease prevention and earlier detection by monitoring health and disease based on an individual’s risk. Active participation in health care can be encouraged with continuous health-monitoring devices, providing a higher-resolution picture of human health and disease. However, the development of monitoring technologies must prioritize the collection of actionable data and long-term user engagement. PMID:29491186

Measurements of hot electrons in the Extrap T1 reversed-field pinch

NASA Astrophysics Data System (ADS)

Welander, A.; Bergsåker, H.

1998-02-01

The presence of an anisotropic energetic electron population in the edge region is a characteristic feature of reversed-field pinch (RFP) plasmas. In the Extrap T1 RFP, the anisotropic, parallel heat flux in the edge region measured by calorimetry was typically several hundred 0741-3335/40/2/011/img1. To gain more insight into the origin of the hot electron component and to achieve time resolution of the hot electron flow during the discharge, a target probe with a soft x-ray monitor was designed, calibrated and implemented. The x-ray emission from the target was measured with a surface barrier detector covered with a set of different x-ray filters to achieve energy resolution. A calibration in the range 0.5-2 keV electron energy was performed on the same target and detector assembly using a 0741-3335/40/2/011/img2 cathode electron gun. The calibration data are interpolated and extrapolated numerically. A directional asymmetry of more than a factor of 100 for the higher energy electrons is observed. The hot electrons are estimated to constitute 10% of the total electron density at the edge and their energy distribution is approximated by a half-Maxwellian with a temperature slightly higher than the central electron temperature. Scalings with plasma current, as well as correlations with local 0741-3335/40/2/011/img3 measurements and radial dependences, are presented.

Whole-brain high in-plane resolution fMRI using accelerated EPIK for enhanced characterisation of functional areas at 3T

PubMed Central

Yun, Seong Dae

2017-01-01

The relatively high imaging speed of EPI has led to its widespread use in dynamic MRI studies such as functional MRI. An approach to improve the performance of EPI, EPI with Keyhole (EPIK), has been previously presented and its use in fMRI was verified at 1.5T as well as 3T. The method has been proven to achieve a higher temporal resolution and smaller image distortions when compared to single-shot EPI. Furthermore, the performance of EPIK in the detection of functional signals was shown to be comparable to that of EPI. For these reasons, we were motivated to employ EPIK here for high-resolution imaging. The method was optimised to offer the highest possible in-plane resolution and slice coverage under the given imaging constraints: fixed TR/TE, FOV and acceleration factors for parallel imaging and partial Fourier techniques. The performance of EPIK was evaluated in direct comparison to the optimised protocol obtained from EPI. The two imaging methods were applied to visual fMRI experiments involving sixteen subjects. The results showed that enhanced spatial resolution with a whole-brain coverage was achieved by EPIK (1.00 mm × 1.00 mm; 32 slices) when compared to EPI (1.25 mm × 1.25 mm; 28 slices). As a consequence, enhanced characterisation of functional areas has been demonstrated in EPIK particularly for relatively small brain regions such as the lateral geniculate nucleus (LGN) and superior colliculus (SC); overall, a significantly increased t-value and activation area were observed from EPIK data. Lastly, the use of EPIK for fMRI was validated with the simulation of different types of data reconstruction methods. PMID:28945780

Voice gender identification by cochlear implant users: The role of spectral and temporal resolution

NASA Astrophysics Data System (ADS)

Fu, Qian-Jie; Chinchilla, Sherol; Nogaki, Geraldine; Galvin, John J.

2005-09-01

The present study explored the relative contributions of spectral and temporal information to voice gender identification by cochlear implant users and normal-hearing subjects. Cochlear implant listeners were tested using their everyday speech processors, while normal-hearing subjects were tested under speech processing conditions that simulated various degrees of spectral resolution, temporal resolution, and spectral mismatch. Voice gender identification was tested for two talker sets. In Talker Set 1, the mean fundamental frequency values of the male and female talkers differed by 100 Hz while in Talker Set 2, the mean values differed by 10 Hz. Cochlear implant listeners achieved higher levels of performance with Talker Set 1, while performance was significantly reduced for Talker Set 2. For normal-hearing listeners, performance was significantly affected by the spectral resolution, for both Talker Sets. With matched speech, temporal cues contributed to voice gender identification only for Talker Set 1 while spectral mismatch significantly reduced performance for both Talker Sets. The performance of cochlear implant listeners was similar to that of normal-hearing subjects listening to 4-8 spectral channels. The results suggest that, because of the reduced spectral resolution, cochlear implant patients may attend strongly to periodicity cues to distinguish voice gender.

Compartmentalized Low-Rank Recovery for High-Resolution Lipid Unsuppressed MRSI

PubMed Central

Bhattacharya, Ipshita; Jacob, Mathews

2017-01-01

Purpose To introduce a novel algorithm for the recovery of high-resolution magnetic resonance spectroscopic imaging (MRSI) data with minimal lipid leakage artifacts, from dual-density spiral acquisition. Methods The reconstruction of MRSI data from dual-density spiral data is formulated as a compartmental low-rank recovery problem. The MRSI dataset is modeled as the sum of metabolite and lipid signals, each of which is support limited to the brain and extracranial regions, respectively, in addition to being orthogonal to each other. The reconstruction problem is formulated as an optimization problem, which is solved using iterative reweighted nuclear norm minimization. Results The comparisons of the scheme against dual-resolution reconstruction algorithm on numerical phantom and in vivo datasets demonstrate the ability of the scheme to provide higher spatial resolution and lower lipid leakage artifacts. The experiments demonstrate the ability of the scheme to recover the metabolite maps, from lipid unsuppressed datasets with echo time (TE)=55 ms. Conclusion The proposed reconstruction method and data acquisition strategy provide an efficient way to achieve high-resolution metabolite maps without lipid suppression. This algorithm would be beneficial for fast metabolic mapping and extension to multislice acquisitions. PMID:27851875

Correlative Super-Resolution Microscopy: New Dimensions and New Opportunities.

PubMed

Hauser, Meghan; Wojcik, Michal; Kim, Doory; Mahmoudi, Morteza; Li, Wan; Xu, Ke

2017-06-14

Correlative microscopy, the integration of two or more microscopy techniques performed on the same sample, produces results that emphasize the strengths of each technique while offsetting their individual weaknesses. Light microscopy has historically been a central method in correlative microscopy due to its widespread availability, compatibility with hydrated and live biological samples, and excellent molecular specificity through fluorescence labeling. However, conventional light microscopy can only achieve a resolution of ∼300 nm, undercutting its advantages in correlations with higher-resolution methods. The rise of super-resolution microscopy (SRM) over the past decade has drastically improved the resolution of light microscopy to ∼10 nm, thus creating exciting new opportunities and challenges for correlative microscopy. Here we review how these challenges are addressed to effectively correlate SRM with other microscopy techniques, including light microscopy, electron microscopy, cryomicroscopy, atomic force microscopy, and various forms of spectroscopy. Though we emphasize biological studies, we also discuss the application of correlative SRM to materials characterization and single-molecule reactions. Finally, we point out current limitations and discuss possible future improvements and advances. We thus demonstrate how a correlative approach adds new dimensions of information and provides new opportunities in the fast-growing field of SRM.

The influence of model grid resolution on estimation of national scale nitrogen deposition and exceedance of critical levels

NASA Astrophysics Data System (ADS)

Dore, A. J.; Kryza, M.; Hall, J. R.; Hallsworth, S.; Keller, V. J. D.; Vieno, M.; Sutton, M. A.

2011-12-01

The Fine Resolution Atmospheric Multi-pollutant Exchange model (FRAME) has been applied to model the spatial distribution of nitrogen deposition and air concentration over the UK at a 1 km spatial resolution. The modelled deposition and concentration data were gridded at resolutions of 1 km, 5 km and 50 km to test the sensitivity of calculations of the exceedance of critical loads for nitrogen deposition to the deposition data resolution. The modelled concentrations of NO2 were validated by comparison with measurements from the rural sites in the national monitoring network and were found to achieve better agreement with the high resolution 1 km data. High resolution plots were found to represent a more physically realistic distribution of nitrogen air concentrations and deposition resulting from use of 1 km resolution precipitation and emissions data as compared to 5 km resolution data. Summary statistics for national scale exceedance of the critical load for nitrogen deposition were not highly sensitive to the grid resolution of the deposition data but did show greater area exceedance with coarser grid resolution due to spatial averaging of high nitrogen deposition hot spots. Local scale deposition at individual Sites of Special Scientific Interest and high precipitation upland sites was sensitive to choice of grid resolution of deposition data. Use of high resolution data tended to generate lower deposition values in sink areas for nitrogen dry deposition (Sites of Scientific Interest) and higher values in high precipitation upland areas. In areas with generally low exceedance (Scotland) and for certain vegetation types (montane), the exceedance statistics were more sensitive to model data resolution.

The influence of model grid resolution on estimation of national scale nitrogen deposition and exceedance of critical loads

NASA Astrophysics Data System (ADS)

Dore, A. J.; Kryza, M.; Hall, J. R.; Hallsworth, S.; Keller, V. J. D.; Vieno, M.; Sutton, M. A.

2012-05-01

The Fine Resolution Atmospheric Multi-pollutant Exchange model (FRAME) was applied to model the spatial distribution of reactive nitrogen deposition and air concentration over the United Kingdom at a 1 km spatial resolution. The modelled deposition and concentration data were gridded at resolutions of 1 km, 5 km and 50 km to test the sensitivity of calculations of the exceedance of critical loads for nitrogen deposition to the deposition data resolution. The modelled concentrations of NO2 were validated by comparison with measurements from the rural sites in the national monitoring network and were found to achieve better agreement with the high resolution 1 km data. High resolution plots were found to represent a more physically realistic distribution of reactive nitrogen air concentrations and deposition resulting from use of 1 km resolution precipitation and emissions data as compared to 5 km resolution data. Summary statistics for national scale exceedance of the critical load for nitrogen deposition were not highly sensitive to the grid resolution of the deposition data but did show greater area exceedance with coarser grid resolution due to spatial averaging of high nitrogen deposition hot spots. Local scale deposition at individual Sites of Special Scientific Interest and high precipitation upland sites was sensitive to choice of grid resolution of deposition data. Use of high resolution data tended to generate lower deposition values in sink areas for nitrogen dry deposition (Sites of Scientific Interest) and higher values in high precipitation upland areas. In areas with generally low exceedance (Scotland) and for certain vegetation types (montane), the exceedance statistics were more sensitive to model data resolution.

An analysis of Landsat Thematic Mapper P-Product internal geometry and conformity to earth surface geometry

NASA Technical Reports Server (NTRS)

Bryant, N. A.; Zobrist, A. L.; Walker, R. E.; Gokhman, B.

1985-01-01

Performance requirements regarding geometric accuracy have been defined in terms of end product goals, but until recently no precise details have been given concerning the conditions under which that accuracy is to be achieved. In order to achieve higher spatial and spectral resolutions, the Thematic Mapper (TM) sensor was designed to image in both forward and reverse mirror sweeps in two separate focal planes. Both hardware and software have been augmented and changed during the course of the Landsat TM developments to achieve improved geometric accuracy. An investigation has been conducted to determine if the TM meets the National Map Accuracy Standards for geometric accuracy at larger scales. It was found that TM imagery, in terms of geometry, has come close to, and in some cases exceeded, its stringent specifications.

Gamma-Ray Focusing Optics for Small Animal Imaging

NASA Technical Reports Server (NTRS)

Pivovaroff, M. J.; Barber, W. C.; Craig, W. W.; Hasegawa, B. H.; Ramsey, B. D.; Taylor, C.

2004-01-01

There is a well-established need for high-resolution radionuclide imaging techniques that provide non-invasive measurement of physiological function in small animals. We, therefore, have begun developing a small animal radionuclide imaging system using grazing incidence mirrors to focus low-energy gamma-rays emitted by I-125, and other radionuclides. Our initial prototype optic, fabricated from thermally-formed glass, demonstrated a resolution of 1500 microns, consistent with the performance predicted by detailed simulations. More recently, we have begun constructing mirrors using a replication technique that reduces low spatial frequency errors in the mirror surface, greatly improving the resolution. Each technique offers particular advantages: e.g., multilayer coatings are easily deposited on glass, while superior resolution is possible with replicated optics. Scaling the results from our prototype optics, which only have a few nested shells, to system where the lens has a full complement of several tens of nested shells, a sensitivity of approx. 1 cps/micro Ci is possible, with the exact number dependent on system magnification and radionuclide species. (Higher levels of efficiency can be obtained with multi-optic imaging systems.) The gamma-ray lens will achieve a resolution as good as 100 microns, independent of the final sensitivity. The combination of high spatial resolution and modest sensitivity will enable in vivo single photon emission imaging studies in small animals.

Improved Hardware for Higher Spatial Resolution Strain-ENCoded (SENC) Breast MRI for Strain Measurements

PubMed Central

Harouni, Ahmed A.; Hossain, Jakir; Jacobs, Michael A.; Osman, Nael F.

2012-01-01

Introduction Early detection of breast lesions using mammography has resulted in lower mortality-rates. However, some breast lesions are mammography occult and magnetic resonance imaging (MRI) is recommended, but has lower specificity. It is possible to achieve higher specificity by using Strain-ENCoded (SENC) MRI and/or magnetic resonance elastography(MRE). SENC breast MRI can measure the strain properties of breast tissue. Similarly, MRE is used to measure elasticity (i.e., shear stiffness) of different tissue compositions interrogating the tissue mechanical properties. Reports have shown that malignant tumors are 3–13 times stiffer than normal tissue and benign tumors. Methods We have developed a Strain-ENCoded (SENC) breast hardware device capable of periodically compressing the breast, thus allowing for longer scanning time and measuring the strain characteristics of breast tissue. This hardware enabled us to use SENC MRI with high spatial resolution (1×1×5mm3) instead of Fast SENC(FSENC). Simple controls and multiple safety measures were added to ensure accurate, repeatable and safe in-vivo experiments. Results Phantom experiments showed that SENC breast MRI has higher SNR and CNR than FSENC under different scanning resolutions. Finally, the SENC breast device reproducibility measurements resulted in a difference of less than one mm with a 1% strain difference. Conclusion SENC breast MR images have higher SNR and CNR than FSENC images. Thus, combining SENC breast strain measurements with diagnostic breast MRI to differentiate benign from malignant lesions could potentially increase the specificity of diagnosis in the clinical setting. PMID:21440464

High spatial resolution restoration of IRAS images

NASA Technical Reports Server (NTRS)

Grasdalen, Gary L.; Inguva, R.; Dyck, H. Melvin; Canterna, R.; Hackwell, John A.

1990-01-01

A general technique to improve the spatial resolution of the IRAS AO data was developed at The Aerospace Corporation using the Maximum Entropy algorithm of Skilling and Gull. The technique has been applied to a variety of fields and several individual AO MACROS. With this general technique, resolutions of 15 arcsec were achieved in 12 and 25 micron images and 30 arcsec in 60 and 100 micron images. Results on galactic plane fields show that both photometric and positional accuracy achieved in the general IRAS survey are also achieved in the reconstructed images.

Atmospheric blocking in the Climate SPHINX simulations: the role of orography and resolution

NASA Astrophysics Data System (ADS)

Davini, Paolo; Corti, Susanna; D'Andrea, Fabio; Riviere, Gwendal; von Hardenberg, Jost

2017-04-01

The representation of atmospheric blocking in numerical simulations, especially over the Euro-Atlantic region, still represents a main concern for the climate modelling community. We here discuss the Northern Hemisphere winter atmospheric blocking representation in a set of 30-year simulations which has been performed in the framework of the PRACE project "Climate SPHINX". Simulations were run using the EC-Earth Global Climate Model with several ensemble members at 5 different horizontal resolutions (ranging from 125 km to 16 km). Results show that the negative bias in blocking frequency over Europe becomes negligible at resolutions of about 40 km and finer. However, the blocking duration is still underestimated by 1-2 days, suggesting that the correct blocking frequencies are achieved with an overestimation of the number of blocking onsets. The reasons leading to such improvements are then discussed, highlighting the role of orography in shaping the Atlantic jet stream: at higher resolution the jet is weaker and less penetrating over Europe, favoring the breaking of synoptic Rossby waves over the Atlantic stationary ridge and thus increasing the simulated blocking frequency.

The impact of recent improvements in cryo-electron microscopy technology on the understanding of bacterial ribosome assembly

PubMed Central

Razi, Aida; Britton, Robert A.

2017-01-01

Abstract Cryo-electron microscopy (cryo-EM) had played a central role in the study of ribosome structure and the process of translation in bacteria since the development of this technique in the mid 1980s. Until recently cryo-EM structures were limited to ∼10 Å in the best cases. However, the recent advent of direct electron detectors has greatly improved the resolution of cryo-EM structures to the point where atomic resolution is now achievable. This improved resolution will allow cryo-EM to make groundbreaking contributions in essential aspects of ribosome biology, including the assembly process. In this review, we summarize important insights that cryo-EM, in combination with chemical and genetic approaches, has already brought to our current understanding of the ribosomal assembly process in bacteria using previous detector technology. More importantly, we discuss how the higher resolution structures now attainable with direct electron detectors can be leveraged to propose precise testable models regarding this process. These structures will provide an effective platform to develop new antibiotics that target this fundamental cellular process. PMID:28180306

Improvements in High Speed, High Resolution Dynamic Digital Image Correlation for Experimental Evaluation of Composite Drive System Components

NASA Technical Reports Server (NTRS)

Kohlman, Lee W.; Ruggeri, Charles R.; Roberts, Gary D.; Handschuh, Robert Frederick

2013-01-01

Composite materials have the potential to reduce the weight of rotating drive system components. However, these components are more complex to design and evaluate than static structural components in part because of limited ability to acquire deformation and failure initiation data during dynamic tests. Digital image correlation (DIC) methods have been developed to provide precise measurements of deformation and failure initiation for material test coupons and for structures under quasi-static loading. Attempts to use the same methods for rotating components (presented at the AHS International 68th Annual Forum in 2012) are limited by high speed camera resolution, image blur, and heating of the structure by high intensity lighting. Several improvements have been made to the system resulting in higher spatial resolution, decreased image noise, and elimination of heating effects. These improvements include the use of a high intensity synchronous microsecond pulsed LED lighting system, different lenses, and changes in camera configuration. With these improvements, deformation measurements can be made during rotating component tests with resolution comparable to that which can be achieved in static tests

Improvements in High Speed, High Resolution Dynamic Digital Image Correlation for Experimental Evaluation of Composite Drive System Components

NASA Technical Reports Server (NTRS)

Kohlman, Lee; Ruggeri, Charles; Roberts, Gary; Handshuh, Robert

2013-01-01

Composite materials have the potential to reduce the weight of rotating drive system components. However, these components are more complex to design and evaluate than static structural components in part because of limited ability to acquire deformation and failure initiation data during dynamic tests. Digital image correlation (DIC) methods have been developed to provide precise measurements of deformation and failure initiation for material test coupons and for structures under quasi-static loading. Attempts to use the same methods for rotating components (presented at the AHS International 68th Annual Forum in 2012) are limited by high speed camera resolution, image blur, and heating of the structure by high intensity lighting. Several improvements have been made to the system resulting in higher spatial resolution, decreased image noise, and elimination of heating effects. These improvements include the use of a high intensity synchronous microsecond pulsed LED lighting system, different lenses, and changes in camera configuration. With these improvements, deformation measurements can be made during rotating component tests with resolution comparable to that which can be achieved in static tests.

Molecular dynamics-based refinement and validation for sub-5 Å cryo-electron microscopy maps

PubMed Central

Singharoy, Abhishek; Teo, Ivan; McGreevy, Ryan; Stone, John E; Zhao, Jianhua; Schulten, Klaus

2016-01-01

Two structure determination methods, based on the molecular dynamics flexible fitting (MDFF) paradigm, are presented that resolve sub-5 Å cryo-electron microscopy (EM) maps with either single structures or ensembles of such structures. The methods, denoted cascade MDFF and resolution exchange MDFF, sequentially re-refine a search model against a series of maps of progressively higher resolutions, which ends with the original experimental resolution. Application of sequential re-refinement enables MDFF to achieve a radius of convergence of ~25 Å demonstrated with the accurate modeling of β-galactosidase and TRPV1 proteins at 3.2 Å and 3.4 Å resolution, respectively. The MDFF refinements uniquely offer map-model validation and B-factor determination criteria based on the inherent dynamics of the macromolecules studied, captured by means of local root mean square fluctuations. The MDFF tools described are available to researchers through an easy-to-use and cost-effective cloud computing resource on Amazon Web Services. DOI: http://dx.doi.org/10.7554/eLife.16105.001 PMID:27383269

Improving Secondary Ion Mass Spectrometry Image Quality with Image Fusion

NASA Astrophysics Data System (ADS)

Tarolli, Jay G.; Jackson, Lauren M.; Winograd, Nicholas

2014-12-01

The spatial resolution of chemical images acquired with cluster secondary ion mass spectrometry (SIMS) is limited not only by the size of the probe utilized to create the images but also by detection sensitivity. As the probe size is reduced to below 1 μm, for example, a low signal in each pixel limits lateral resolution because of counting statistics considerations. Although it can be useful to implement numerical methods to mitigate this problem, here we investigate the use of image fusion to combine information from scanning electron microscope (SEM) data with chemically resolved SIMS images. The advantage of this approach is that the higher intensity and, hence, spatial resolution of the electron images can help to improve the quality of the SIMS images without sacrificing chemical specificity. Using a pan-sharpening algorithm, the method is illustrated using synthetic data, experimental data acquired from a metallic grid sample, and experimental data acquired from a lawn of algae cells. The results show that up to an order of magnitude increase in spatial resolution is possible to achieve. A cross-correlation metric is utilized for evaluating the reliability of the procedure.

Pushing the limits of sulfur SAD phasing: de novo structure solution of the N-terminal domain of the ectodomain of HCV E1

DOE Office of Scientific and Technical Information (OSTI.GOV)

El Omari, Kamel; Iourin, Oleg; Kadlec, Jan

2014-08-01

The sulfur SAD phasing method was successfully used to determine the structure of the N-terminal domain of HCV E1 from low-resolution diffracting crystals by combining data from 32 crystals. Single-wavelength anomalous dispersion of S atoms (S-SAD) is an elegant phasing method to determine crystal structures that does not require heavy-atom incorporation or selenomethionine derivatization. Nevertheless, this technique has been limited by the paucity of the signal at the usual X-ray wavelengths, requiring very accurate measurement of the anomalous differences. Here, the data collection and structure solution of the N-terminal domain of the ectodomain of HCV E1 from crystals that diffractedmore » very weakly is reported. By combining the data from 32 crystals, it was possible to solve the sulfur substructure and calculate initial maps at 7 Å resolution, and after density modication and phase extension using a higher resolution native data set to 3.5 Å resolution model building was achievable.« less

Application of Sensor Fusion to Improve Uav Image Classification

NASA Astrophysics Data System (ADS)

Jabari, S.; Fathollahi, F.; Zhang, Y.

2017-08-01

Image classification is one of the most important tasks of remote sensing projects including the ones that are based on using UAV images. Improving the quality of UAV images directly affects the classification results and can save a huge amount of time and effort in this area. In this study, we show that sensor fusion can improve image quality which results in increasing the accuracy of image classification. Here, we tested two sensor fusion configurations by using a Panchromatic (Pan) camera along with either a colour camera or a four-band multi-spectral (MS) camera. We use the Pan camera to benefit from its higher sensitivity and the colour or MS camera to benefit from its spectral properties. The resulting images are then compared to the ones acquired by a high resolution single Bayer-pattern colour camera (here referred to as HRC). We assessed the quality of the output images by performing image classification tests. The outputs prove that the proposed sensor fusion configurations can achieve higher accuracies compared to the images of the single Bayer-pattern colour camera. Therefore, incorporating a Pan camera on-board in the UAV missions and performing image fusion can help achieving higher quality images and accordingly higher accuracy classification results.

LOFAR 150-MHz observations of the Boötes field: catalogue and source counts

NASA Astrophysics Data System (ADS)

Williams, W. L.; van Weeren, R. J.; Röttgering, H. J. A.; Best, P.; Dijkema, T. J.; de Gasperin, F.; Hardcastle, M. J.; Heald, G.; Prandoni, I.; Sabater, J.; Shimwell, T. W.; Tasse, C.; van Bemmel, I. M.; Brüggen, M.; Brunetti, G.; Conway, J. E.; Enßlin, T.; Engels, D.; Falcke, H.; Ferrari, C.; Haverkorn, M.; Jackson, N.; Jarvis, M. J.; Kapińska, A. D.; Mahony, E. K.; Miley, G. K.; Morabito, L. K.; Morganti, R.; Orrú, E.; Retana-Montenegro, E.; Sridhar, S. S.; Toribio, M. C.; White, G. J.; Wise, M. W.; Zwart, J. T. L.

2016-08-01

We present the first wide area (19 deg2), deep (≈120-150 μJy beam-1), high-resolution (5.6 × 7.4 arcsec) LOFAR High Band Antenna image of the Boötes field made at 130-169 MHz. This image is at least an order of magnitude deeper and 3-5 times higher in angular resolution than previously achieved for this field at low frequencies. The observations and data reduction, which includes full direction-dependent calibration, are described here. We present a radio source catalogue containing 6 276 sources detected over an area of 19 deg2, with a peak flux density threshold of 5σ. As the first thorough test of the facet calibration strategy, introduced by van Weeren et al., we investigate the flux and positional accuracy of the catalogue. We present differential source counts that reach an order of magnitude deeper in flux density than previously achieved at these low frequencies, and show flattening at 150-MHz flux densities below 10 mJy associated with the rise of the low flux density star-forming galaxies and radio-quiet AGN.

Femtosecond Electron Wave Packet Propagation and Diffraction: Towards Making the ``Molecular Movie"

NASA Astrophysics Data System (ADS)

Miller, R. J. Dwayne

2003-03-01

Time-resolved electron diffraction harbors great promise for achieving atomic resolution of the fastest chemical processes. The generation of sufficiently short electron pulses to achieve this real time view of a chemical reaction has been limited by problems in maintaining short electron pulses with realistic electron densities to the sample. The propagation dynamics of femtosecond electron packets in the drift region of a photoelectron gun are investigated with an N-body numerical simulation and mean-field model. This analyis shows that the redistribution of electrons inside the packet, arising from space-charge and dispersion contributions, changes the pulse envelope and leads to the development of a spatially linear axial velocity distribution. These results have been used in the design of femtosecond photoelectron guns with higher time resolution and novel electron-optical methods of pulse characterization that are approaching 100 fs timescales. Time-resolved diffraction studies with electron pulses of approximately 500 femtoseconds have focused on solid-liquid phase transitions under far from equilibrium conditions. This work gives a microscopic description of the melting process and illustrates the promise of atomically resolving transition state processes.

Position Sensitive Proximity Charge Sensing Readout of HPGe Detectors

NASA Astrophysics Data System (ADS)

Priest, Anders Peterson

Electrode segmentation is a necessity to achieve position sensitivity in semicon- ductor radiation detectors. Traditional segmentation requires decreasing electrode sizes while increasing channel numbers to achieve very fine position resolution. These electrodes can be complicated to fabricate, and many electrodes with individual electronic channels are required to instrument large detector areas. To simplify the fabrication process, we have moved the readout electrodes onto a printed circuit board that is positioned above the ionization type detection material. In this scheme, charge from radiation interactions will be shared amongst several electrodes, allowing for position interpolation. Because events can be reconstructed in between electrodes, fewer electrodes are needed to instrument large detector areas. The proximity charge sensing method of readout promises to simplify detector fabrication while maintaining the position resolution that is required by fields such as homeland security, astrophysics, environmental remediation, nuclear physics, and medical imaging. We performed scanning measurements on a proof of principle detector that we fabricated at Lawrence Berkeley National Laboratory (LBNL). These measurements showed that position resolution much finer than the strip pitch was achievable using the proximity charge readout method. We performed analytic calculations and Monte Carlo modeling to optimize the readout electrode geometry for a larger detector to test the limits of this technology. We achieved an average position resolution of 288 microm with eight proximity electrodes at a 5 mm pitch and 1 mm strip width, set 100 microm away from the detector surface by a Kapton spacer. To achieve this resolution using standard technologies, 300 microm pitch strips are necessary, and would require 100 channels to instrument the same area. Through our optimization calculations, we found that there is a trade-off between position resolution and energy resolution, and this system provided comparatively poor energy resolution by HPGe standards, with 4.7 keV FWHM at 59.5 keV. With another electrode geometry, we were able to achieve 2.9 keV FWHM at 59.5 keV. This dissertation describes the work we completed to achieve these results.

A novel Fast Gas Chromatography based technique for higher time resolution measurements of speciated monoterpenes in air

NASA Astrophysics Data System (ADS)

Jones, C. E.; Kato, S.; Nakashima, Y.; Kajii, Y.

2013-12-01

Biogenic emissions supply the largest fraction of non-methane volatile organic compounds (VOC) from the biosphere to the atmospheric boundary layer, and typically comprise a complex mixture of reactive terpenes. Due to this chemical complexity, achieving comprehensive measurements of biogenic VOC (BVOC) in air within a satisfactory time resolution is analytically challenging. To address this, we have developed a novel, fully automated Fast Gas Chromatography (Fast-GC) based technique to provide higher time resolution monitoring of monoterpenes (and selected other C9-C15 terpenes) during plant emission studies and in ambient air. To our knowledge, this is the first study to apply a Fast-GC based separation technique to achieve quantification of terpenes in air. Three chromatography methods have been developed for atmospheric terpene analysis under different sampling scenarios. Each method facilitates chromatographic separation of selected BVOC within a significantly reduced analysis time compared to conventional GC methods, whilst maintaining the ability to quantify individual monoterpene structural isomers. Using this approach, the C10-C15 BVOC composition of single plant emissions may be characterised within a ~ 14 min analysis time. Moreover, in situ quantification of 12 monoterpenes in unpolluted ambient air may be achieved within an ~ 11 min chromatographic separation time (increasing to ~ 19 min when simultaneous quantification of multiple oxygenated C9-C10 terpenoids is required, and/or when concentrations of anthropogenic VOC are significant). This corresponds to a two- to fivefold increase in measurement frequency compared to conventional GC methods. Here we outline the technical details and analytical capability of this chromatographic approach, and present the first in situ Fast-GC observations of 6 monoterpenes and the oxygenated BVOC linalool in ambient air. During this field deployment within a suburban forest ~ 30 km west of central Tokyo, Japan, the Fast-GC limit of detection with respect to monoterpenes was 4-5 ppt, and the agreement between Fast-GC and PTR-MS derived total monoterpene mixing ratios was consistent with previous GC/PTR-MS comparisons. The measurement uncertainties associated with the Fast-GC quantification of monoterpenes are ≤ 10%, while larger uncertainties (up to ~ 25%) are associated with the OBVOC and sesquiterpene measurements.

Quantum state atomic force microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Passian, Ali; Siopsis, George

New classical modalities of atomic force microscopy continue to emerge to achieve higher spatial, spectral, and temporal resolution for nanometrology of materials. Here, we introduce the concept of a quantum mechanical modality that capitalizes on squeezed states of probe displacement. We show that such squeezing is enabled nanomechanically when the probe enters the van der Waals regime of interaction with a sample. The effect is studied in the non-contact mode, where we consider the parameter domains characterizing the attractive regime of the probe-sample interaction force.

Quantum state atomic force microscopy

DOE PAGES

Passian, Ali; Siopsis, George

2017-04-10

New classical modalities of atomic force microscopy continue to emerge to achieve higher spatial, spectral, and temporal resolution for nanometrology of materials. Here, we introduce the concept of a quantum mechanical modality that capitalizes on squeezed states of probe displacement. We show that such squeezing is enabled nanomechanically when the probe enters the van der Waals regime of interaction with a sample. The effect is studied in the non-contact mode, where we consider the parameter domains characterizing the attractive regime of the probe-sample interaction force.

Estimating Morning Change in Land Surface Temperature from MODIS Day/Night Observations: Applications for Surface Energy Balance Modeling.

PubMed

Hain, Christopher R; Anderson, Martha C

2017-10-16

Observations of land surface temperature (LST) are crucial for the monitoring of surface energy fluxes from satellite. Methods that require high temporal resolution LST observations (e.g., from geostationary orbit) can be difficult to apply globally because several geostationary sensors are required to attain near-global coverage (60°N to 60°S). While these LST observations are available from polar-orbiting sensors, providing global coverage at higher spatial resolutions, the temporal sampling (twice daily observations) can pose significant limitations. For example, the Atmosphere Land Exchange Inverse (ALEXI) surface energy balance model, used for monitoring evapotranspiration and drought, requires an observation of the morning change in LST - a quantity not directly observable from polar-orbiting sensors. Therefore, we have developed and evaluated a data-mining approach to estimate the mid-morning rise in LST from a single sensor (2 observations per day) of LST from the Moderate Resolution Imaging Spectroradiometer (MODIS) sensor on the Aqua platform. In general, the data-mining approach produced estimates with low relative error (5 to 10%) and statistically significant correlations when compared against geostationary observations. This approach will facilitate global, near real-time applications of ALEXI at higher spatial and temporal coverage from a single sensor than currently achievable with current geostationary datasets.

Nonlinear vibrational microscopy

DOEpatents

Holtom, Gary R.; Xie, Xiaoliang Sunney; Zumbusch, Andreas

2000-01-01

The present invention is a method and apparatus for microscopic vibrational imaging using coherent Anti-Stokes Raman Scattering or Sum Frequency Generation. Microscopic imaging with a vibrational spectroscopic contrast is achieved by generating signals in a nonlinear optical process and spatially resolved detection of the signals. The spatial resolution is attained by minimizing the spot size of the optical interrogation beams on the sample. Minimizing the spot size relies upon a. directing at least two substantially co-axial laser beams (interrogation beams) through a microscope objective providing a focal spot on the sample; b. collecting a signal beam together with a residual beam from the at least two co-axial laser beams after passing through the sample; c. removing the residual beam; and d. detecting the signal beam thereby creating said pixel. The method has significantly higher spatial resolution then IR microscopy and higher sensitivity than spontaneous Raman microscopy with much lower average excitation powers. CARS and SFG microscopy does not rely on the presence of fluorophores, but retains the resolution and three-dimensional sectioning capability of confocal and two-photon fluorescence microscopy. Complementary to these techniques, CARS and SFG microscopy provides a contrast mechanism based on vibrational spectroscopy. This vibrational contrast mechanism, combined with an unprecedented high sensitivity at a tolerable laser power level, provides a new approach for microscopic investigations of chemical and biological samples.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Parkin, E. R.; Bicknell, G. V., E-mail: parkin@mso.anu.edu.au

Global three-dimensional magnetohydrodynamic (MHD) simulations of turbulent accretion disks are presented which start from fully equilibrium initial conditions in which the magnetic forces are accounted for and the induction equation is satisfied. The local linear theory of the magnetorotational instability (MRI) is used as a predictor of the growth of magnetic field perturbations in the global simulations. The linear growth estimates and global simulations diverge when nonlinear motions-perhaps triggered by the onset of turbulence-upset the velocity perturbations used to excite the MRI. The saturated state is found to be independent of the initially excited MRI mode, showing that once themore » disk has expelled the initially net flux field and settled into quasi-periodic oscillations in the toroidal magnetic flux, the dynamo cycle regulates the global saturation stress level. Furthermore, time-averaged measures of converged turbulence, such as the ratio of magnetic energies, are found to be in agreement with previous works. In particular, the globally averaged stress normalized to the gas pressure <{alpha}{sub P}>bar = 0.034, with notably higher values achieved for simulations with higher azimuthal resolution. Supplementary tests are performed using different numerical algorithms and resolutions. Convergence with resolution during the initial linear MRI growth phase is found for 23-35 cells per scale height (in the vertical direction).« less

Silicon Detector System for High Rate EXAFS Applications.

PubMed

Pullia, A; Kraner, H W; Siddons, D P; Furenlid, L R; Bertuccio, G

1995-08-01

A multichannel silicon pad detector for EXAFS (Extended X-ray Absorption Fine Structure) applications has been designed and built. The X-ray spectroscopic measurements demonstrate that an adequate energy resolution of 230 eV FWHM (corresponding to 27 rms electrons in silicon) can be achieved reliably at -35 °C. A resolution of 190 eV FWHM (corresponding to 22 rms electrons) has been obtained from individual pads at -35 °C. At room temperature (25 °C) an average energy resolution of 380 eV FWHM is achieved and a resolution of 350 eV FWHM (41 rms electrons) is the best performance. A simple cooling system constituted of Peltier cells is sufficient to reduce the reverse currents of the pads and their related shot noise contribution, in order to achieve resolutions better than 300 eV FWHM which is adequate for the EXAFS applications.

Silicon Detector System for High Rate EXAFS Applications

PubMed Central

Pullia, A.; Kraner, H. W.; Siddons, D. P.; Furenlid, L. R.; Bertuccio, G.

2015-01-01

A multichannel silicon pad detector for EXAFS (Extended X-ray Absorption Fine Structure) applications has been designed and built. The X-ray spectroscopic measurements demonstrate that an adequate energy resolution of 230 eV FWHM (corresponding to 27 rms electrons in silicon) can be achieved reliably at −35 °C. A resolution of 190 eV FWHM (corresponding to 22 rms electrons) has been obtained from individual pads at −35 °C. At room temperature (25 °C) an average energy resolution of 380 eV FWHM is achieved and a resolution of 350 eV FWHM (41 rms electrons) is the best performance. A simple cooling system constituted of Peltier cells is sufficient to reduce the reverse currents of the pads and their related shot noise contribution, in order to achieve resolutions better than 300 eV FWHM which is adequate for the EXAFS applications. PMID:26538683

High-resolution three-dimensional visualization of the rat spinal cord microvasculature by synchrotron radiation micro-CT

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hu, Jianzhong; Cao, Yong; Wu, Tianding

2014-10-15

Purpose: Understanding the three-dimensional (3D) morphology of the spinal cord microvasculature has been limited by the lack of an effective high-resolution imaging technique. In this study, synchrotron radiation microcomputed tomography (SRµCT), a novel imaging technique based on absorption imaging, was evaluated with regard to the detection of the 3D morphology of the rat spinal cord microvasculature. Methods: Ten Sprague-Dawley rats were used in this ex vivo study. After contrast agent perfusion, their spinal cords were isolated and scanned using conventional x-rays, conventional micro-CT (CµCT), and SRµCT. Results: Based on contrast agent perfusion, the microvasculature of the rat spinal cord wasmore » clearly visualized for the first time ex vivo in 3D by means of SRµCT scanning. Compared to conventional imaging techniques, SRµCT achieved higher resolution 3D vascular imaging, with the smallest vessel that could be distinguished approximately 7.4 μm in diameter. Additionally, a 3D pseudocolored image of the spinal cord microvasculature was generated in a single session of SRµCT imaging, which was conducive to detailed observation of the vessel morphology. Conclusions: The results of this study indicated that SRµCT scanning could provide higher resolution images of the vascular network of the spinal cord. This modality also has the potential to serve as a powerful imaging tool for the investigation of morphology changes in the 3D angioarchitecture of the neurovasculature in preclinical research.« less

High spatial resolution shortwave infrared imaging technology based on time delay and digital accumulation method

NASA Astrophysics Data System (ADS)

Jia, Jianxin; Wang, Yueming; Zhuang, Xiaoqiong; Yao, Yi; Wang, Shengwei; Zhao, Ding; Shu, Rong; Wang, Jianyu

2017-03-01

Shortwave infrared (SWIR) imaging technology attracts more and more attention by its fascinating ability of penetrating haze and smoke. For application of spaceborne remote sensing, spatial resolution of SWIR is lower compared with that of visible light (VIS) wavelength. It is difficult to balance between the spatial resolution and signal to noise ratio (SNR). Some conventional methods, such as enlarging aperture of telescope, image motion compensation, and analog time delay and integration (TDI) technology are used to gain SNR. These techniques bring in higher cost of satellite, complexity of system or other negative factors. In this paper, time delay and digital accumulation (TDDA) method is proposed to achieve higher spatial resolution. The method can enhance the SNR and non-uniformity of system theoretically. A prototype of SWIR imager consists of opto-mechanical, 1024 × 128 InGaAs detector, and electronics is designed and integrated to prove TDDA method. Both of measurements and experimental results indicate TDDA method can promote SNR of system approximated of the square root of accumulative stage. The results exhibit that non-uniformity of system is also improved by this approach to some extent. The experiment results are corresponded with the theoretical analysis. Based on the experiments results, it is proved firstly that the goal of 1 m ground sample distance (GSD) in orbit of 500 km is feasible with the TDDA stage of 30 for SWIR waveband (0.9-1.7 μm).

Development of Compton X-ray spectrometer for high energy resolution single-shot high-flux hard X-ray spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kojima, Sadaoki, E-mail: kojima-s@ile.osaka-u.ac.jp, E-mail: sfujioka@ile.osaka-u.ac.jp; Ikenouchi, Takahito; Arikawa, Yasunobu

Hard X-ray spectroscopy is an essential diagnostics used to understand physical processes that take place in high energy density plasmas produced by intense laser-plasma interactions. A bundle of hard X-ray detectors, of which the responses have different energy thresholds, is used as a conventional single-shot spectrometer for high-flux (>10{sup 13} photons/shot) hard X-rays. However, high energy resolution (Δhv/hv < 0.1) is not achievable with a differential energy threshold (DET) X-ray spectrometer because its energy resolution is limited by energy differences between the response thresholds. Experimental demonstration of a Compton X-ray spectrometer has already been performed for obtaining higher energy resolutionmore » than that of DET spectrometers. In this paper, we describe design details of the Compton X-ray spectrometer, especially dependence of energy resolution and absolute response on photon-electron converter design and its background reduction scheme, and also its application to the laser-plasma interaction experiment. The developed spectrometer was used for spectroscopy of bremsstrahlung X-rays generated by intense laser-plasma interactions using a 200 μm thickness SiO{sub 2} converter. The X-ray spectrum obtained with the Compton X-ray spectrometer is consistent with that obtained with a DET X-ray spectrometer, furthermore higher certainly of a spectral intensity is obtained with the Compton X-ray spectrometer than that with the DET X-ray spectrometer in the photon energy range above 5 MeV.« less

Impact of respiratory motion correction and spatial resolution on lesion detection in PET: a simulation study based on real MR dynamic data

NASA Astrophysics Data System (ADS)

Polycarpou, Irene; Tsoumpas, Charalampos; King, Andrew P.; Marsden, Paul K.

2014-02-01

The aim of this study is to investigate the impact of respiratory motion correction and spatial resolution on lesion detectability in PET as a function of lesion size and tracer uptake. Real respiratory signals describing different breathing types are combined with a motion model formed from real dynamic MR data to simulate multiple dynamic PET datasets acquired from a continuously moving subject. Lung and liver lesions were simulated with diameters ranging from 6 to 12 mm and lesion to background ratio ranging from 3:1 to 6:1. Projection data for 6 and 3 mm PET scanner resolution were generated using analytic simulations and reconstructed without and with motion correction. Motion correction was achieved using motion compensated image reconstruction. The detectability performance was quantified by a receiver operating characteristic (ROC) analysis obtained using a channelized Hotelling observer and the area under the ROC curve (AUC) was calculated as the figure of merit. The results indicate that respiratory motion limits the detectability of lung and liver lesions, depending on the variation of the breathing cycle length and amplitude. Patients with large quiescent periods had a greater AUC than patients with regular breathing cycles and patients with long-term variability in respiratory cycle or higher motion amplitude. In addition, small (less than 10 mm diameter) or low contrast (3:1) lesions showed the greatest improvement in AUC as a result of applying motion correction. In particular, after applying motion correction the AUC is improved by up to 42% with current PET resolution (i.e. 6 mm) and up to 51% for higher PET resolution (i.e. 3 mm). Finally, the benefit of increasing the scanner resolution is small unless motion correction is applied. This investigation indicates high impact of respiratory motion correction on lesion detectability in PET and highlights the importance of motion correction in order to benefit from the increased resolution of future PET scanners.

A design method for high performance seismic data acquisition based on oversampling delta-sigma modulation

NASA Astrophysics Data System (ADS)

Gao, Shanghua; Xue, Bing

2017-04-01

The dynamic range of the currently most widely used 24-bit seismic data acquisition devices is 10-20 dB lower than that of broadband seismometers, and this can affect the completeness of seismic waveform recordings under certain conditions. However, this problem is not easy to solve because of the lack of analog to digital converter (ADC) chips with more than 24 bits in the market. So the key difficulties for higher-resolution data acquisition devices lie in achieving more than 24-bit ADC circuit. In the paper, we propose a method in which an adder, an integrator, a digital to analog converter chip, a field-programmable gate array, and an existing low-resolution ADC chip are used to build a third-order 16-bit oversampling delta-sigma modulator. This modulator is equipped with a digital decimation filter, thus forming a complete analog to digital converting circuit. Experimental results show that, within the 0.1-40 Hz frequency range, the circuit board's dynamic range reaches 158.2 dB, its resolution reaches 25.99 dB, and its linearity error is below 2.5 ppm, which is better than what is achieved by the commercial 24-bit ADC chips ADS1281 and CS5371. This demonstrates that the proposed method may alleviate or even solve the amplitude-limitation problem that broadband observation systems so commonly have to face during strong earthquakes.

Synthetic light-needle photoacoustic microscopy for extended depth of field (Conference Presentation)

NASA Astrophysics Data System (ADS)

Yang, Jiamiao; Gong, Lei; Xu, Xiao; Hai, Pengfei; Suzuki, Yuta; Wang, Lihong V.

2017-03-01

Photoacoustic microscopy (PAM) has been extensively applied in biomedical study because of its ability to visualize tissue morphology and physiology in vivo in three dimensions (3D). However, conventional PAM suffers from a rapidly decreasing resolution away from the focal plane because of the limited depth of focus of an objective lens, which deteriorates the volumetric imaging quality inevitably. Here, we propose a novel method to synthesize an ultra-long light needle to extend a microscope's depth of focus beyond its physical limitations with wavefront engineering method. Furthermore, it enables an improved lateral resolution that exceeds the diffraction limit of the objective lens. The virtual light needle can be flexibly synthesized anywhere throughout the imaging volume without mechanical scanning. Benefiting from these advantages, we developed a synthetic light needle photoacoustic microscopy (SLN-PAM) to achieve an extended depth of field (DOF), sub-diffraction and motionless volumetric imaging. The DOF of our SLN-PAM system is up to 1800 µm, more than 30-fold improvement over that gained by conventional PAM. Our system also achieves the lateral resolution of 1.8 µm (characterized at 532 nm and 0.1 NA objective), about 50% higher than the Rayleigh diffraction limit. Its superior imaging performance was demonstrated by 3D imaging of both non-biological and biological samples. This extended DOF, sub-diffraction and motionless 3D PAM will open up new opportunities for potential biomedical applications.

An Overview of the HST Advanced Camera for Surveys' On-orbit Performance

NASA Astrophysics Data System (ADS)

Hartig, G. F.; Ford, H. C.; Illingworth, G. D.; Clampin, M.; Bohlin, R. C.; Cox, C.; Krist, J.; Sparks, W. B.; De Marchi, G.; Martel, A. R.; McCann, W. J.; Meurer, G. R.; Sirianni, M.; Tsvetanov, Z.; Bartko, F.; Lindler, D. J.

2002-05-01

The Advanced Camera for Surveys (ACS) was installed in the HST on 7 March 2002 during the fourth servicing mission to the observatory, and is now beginning science operations. The ACS provides HST observers with a considerably more sensitive, higher-resolution camera with wider field and polarimetric, coronagraphic, low-resolution spectrographic and solar-blind FUV capabilities. We review selected results of the early verification and calibration program, comparing the achieved performance with the advertised specifications. Emphasis is placed on the optical characteristics of the camera, including image quality, throughput, geometric distortion and stray-light performance. More detailed analyses of various aspects of the ACS performance are presented in other papers at this meeting. This work was supported by a NASA contract and a NASA grant.

Novel Electrochemical Raman Spectroscopy Enabled by Water Immersion Objective.

PubMed

Zeng, Zhi-Cong; Hu, Shu; Huang, Sheng-Chao; Zhang, Yue-Jiao; Zhao, Wei-Xing; Li, Jian-Feng; Jiang, Chaoyang; Ren, Bin

2016-10-04

Electrochemical Raman spectroscopy is a powerful molecular level diagnostic technique for in situ investigation of adsorption and reactions on various material surfaces. However, there is still a big room to improve the optical path to meet the increasing request of higher detection sensitivity and spatial resolution. Herein, we proposed a novel electrochemical Raman setup based on a water immersion objective. It dramatically reduces mismatch of the refractive index in the light path. Consequently, significant improvement in detection sensitivity and spatial resolution has been achieved from both Zemax simulation and the experimental results. Furthermore, the thickness of electrolyte layer could be expanded to 2 mm without any influence on the signal collection. Such a thick electrolyte layer allows a much normal electrochemical response during the spectroelectrochemical investigations of the methanol oxidation.

Optimization of niobium tunnel junctions as X-ray detectors

NASA Technical Reports Server (NTRS)

Saulnier, Gregory G.; Zacher, Robert A.; Van Vechten, Deborah; Boyer, Craig; Lovellette, Michael N.; Fritz, Gilbert G.; Soulen, Robert J.; Kang, Joonhee; Blamire, Mark; Kirk, Eugenie C. G.

1992-01-01

We report on our ongoing work using Nb/Al/AlO(x)/Nb junctions for the detection of X-rays. Detectors based on superconducting tunneling junctions offer the prospect of resolution over an order of magnitude higher than is obtainable with the current generation of semiconductor-based detectors. Results of measurements taken at 1.85 K (a temperature achievable with current space flight technology) include the current-voltage (I-V) curve, subgap current vs temperature, the dependence of the superconducting current on the applied magnetic field (Fraunhofer pattern), X-ray pulses, and the spectra from a 6 keV X-ray source which gave an intrinsic device resolution of approximately 700 eV. The collection of more than 10 exp 5 electrons per 6 keV photon is established.

Dilation x-ray imager a new∕faster gated x-ray imager for the NIF.

PubMed

Nagel, S R; Hilsabeck, T J; Bell, P M; Bradley, D K; Ayers, M J; Barrios, M A; Felker, B; Smith, R F; Collins, G W; Jones, O S; Kilkenny, J D; Chung, T; Piston, K; Raman, K S; Sammuli, B; Hares, J D; Dymoke-Bradshaw, A K L

2012-10-01

As the yield on implosion shots increases it is expected that the peak x-ray emission reduces to a duration with a FWHM as short as 20 ps for ∼7 × 10(18) neutron yield. However, the temporal resolution of currently used gated x-ray imagers on the NIF is 40-100 ps. We discuss the benefits of the higher temporal resolution for the NIF and present performance measurements for dilation x-ray imager, which utilizes pulse-dilation technology [T. J. Hilsabeck et al., Rev. Sci. Instrum. 81, 10E317 (2010)] to achieve x-ray imaging with temporal gate times below 10 ps. The measurements were conducted using the COMET laser, which is part of the Jupiter Laser Facility at the Lawrence Livermore National Laboratory.

Absorption-enhanced imaging through scattering media using carbon black nano-particles: from visible to near infrared wavelengths

NASA Astrophysics Data System (ADS)

Tanzid, Mehbuba; Hogan, Nathaniel J.; Robatjazi, Hossein; Veeraraghavan, Ashok; Halas, Naomi J.

2018-05-01

Imaging through scattering media can be improved with the addition of absorbers, since multiply-scattered photons, with their longer path length, are absorbed with a higher probability than ballistic photons. The image resolution enhancement is substantially greater when imaging through isotropic scatterers than when imaging through an ensemble of strongly forward-scattering particles. However, since the angular scattering distribution is determined by the size of the scatterers with respect to the wavelength of incident light, particles that are forward scatterers at visible wavelengths can be isotropic scatterers at infrared (IR) wavelengths. Here, we show that substantial image resolution enhancement can be achieved in the near-infrared wavelength regime for particles that are forward scattering at visible wavelengths using carbon black nanoparticles as a broadband absorber. This observation provides a new strategy for image enhancement through scattering media: by selecting the appropriate wavelength range for imaging, in this case the near-IR, the addition of absorbers more effectively enhances the image resolution.

High-Resolution Hard X-Ray and Gamma-Ray Spectrometers Based on Superconducting Absorbers Coupled to Superconducting Transition Edge Sensors

DOE Office of Scientific and Technical Information (OSTI.GOV)

van den Berg, M.; Chow, D.; Loshak, A.

2000-09-21

We are developing detectors based on bulk superconducting absorbers coupled to superconducting transition edge sensors (TES) for high-resolution spectroscopy of hard X-rays and soft gamma-rays. We have achieved an energy resolution of 70 eV FWHM at 60 keV using a 1 x 1 x 0.25 mm{sup 3} Sn absorber coupled to a Mo/Cu multilayer TES with a transition temperature of 100 mK. The response of the detector is compared with a simple model using only material properties data and characteristics derived from IV-measurements. We have also manufactured detectors using superconducting absorbers with a higher stopping power, such as Pb andmore » Ta. We present our first measurements of these detectors, including the thermalization characteristics of the bulk superconducting absorbers. The differences in performance between the detectors are discussed and an outline of the future direction of our detector development efforts is given.« less

In vivo correlation mapping microscopy

NASA Astrophysics Data System (ADS)

McGrath, James; Alexandrov, Sergey; Owens, Peter; Subhash, Hrebesh; Leahy, Martin

2016-04-01

To facilitate regular assessment of the microcirculation in vivo, noninvasive imaging techniques such as nailfold capillaroscopy are required in clinics. Recently, a correlation mapping technique has been applied to optical coherence tomography (OCT), which extends the capabilities of OCT to microcirculation morphology imaging. This technique, known as correlation mapping optical coherence tomography, has been shown to extract parameters, such as capillary density and vessel diameter, and key clinical markers associated with early changes in microvascular diseases. However, OCT has limited spatial resolution in both the transverse and depth directions. Here, we extend this correlation mapping technique to other microscopy modalities, including confocal microscopy, and take advantage of the higher spatial resolution offered by these modalities. The technique is achieved as a processing step on microscopy images and does not require any modification to the microscope hardware. Results are presented which show that this correlation mapping microscopy technique can extend the capabilities of conventional microscopy to enable mapping of vascular networks in vivo with high spatial resolution in both the transverse and depth directions.

Achieving High Resolution Timer Events in Virtualized Environment.

PubMed

Adamczyk, Blazej; Chydzinski, Andrzej

2015-01-01

Virtual Machine Monitors (VMM) have become popular in different application areas. Some applications may require to generate the timer events with high resolution and precision. This however may be challenging due to the complexity of VMMs. In this paper we focus on the timer functionality provided by five different VMMs-Xen, KVM, Qemu, VirtualBox and VMWare. Firstly, we evaluate resolutions and precisions of their timer events. Apparently, provided resolutions and precisions are far too low for some applications (e.g. networking applications with the quality of service). Then, using Xen virtualization we demonstrate the improved timer design that greatly enhances both the resolution and precision of achieved timer events.

Room temperature synthesis of rutile nanorods and their applications on cloth

NASA Astrophysics Data System (ADS)

Fei, Bin; Deng, Zhaoxiang; Xin, John H.; Zhang, Yihe; Pang, Geoffrey

2006-04-01

In order to achieve better photocatalytic performance, rutile nanorods dispersed in anatase and brookite phases were synthesized from titanium isopropoxide (TIP) in a concentrated HNO3 solution at room temperature (23 °C). X-ray diffraction results indicated that the percentage of rutile increased with increasing peptization time. Scanning electron microscopy and and high-resolution transmission electron microscopy measurements revealed that the nanosized titania particles mainly consisted of granular anatase and brookite, and rod-like rutile. It was interesting that the stability of the colloid increased with increasing nanoparticle concentration, and the tricrystalline titania showed a photocatalytic activity higher than that of pure anatase. These nanocrystals were applied onto cotton fabrics, and achieved a promising bactericidal photocatalytic activity and excellent protection against UV radiation.

A Spread-Spectrum SQUID Multiplexer

NASA Astrophysics Data System (ADS)

Irwin, K. D.; Chaudhuri, S.; Cho, H.-M.; Dawson, C.; Kuenstner, S.; Li, D.; Titus, C. J.; Young, B. A.

2018-06-01

The transition-edge sensor (TES) is a mature, high-resolution x-ray spectrometer technology that provides a much higher efficiency than dispersive spectrometers such as gratings and crystal spectrometers. As larger arrays are developed, time-division multiplexing schemes operating at MHz frequencies are being replaced by microwave SQUID multiplexers using frequency-division multiplexing at GHz frequencies. However, the multiplexing factor achievable with microwave SQUIDs is limited by the high slew rate on the leading edge of x-ray pulses. In this paper, we propose a new multiplexing scheme for high-slew-rate TES x-ray calorimeters: the spread-spectrum SQUID multiplexer, which has the potential to enable higher multiplexing factors, especially in applications with lower photon-arrival rates.

High Efficiency Multi-shot Interleaved Spiral-In/Out Acquisition for High Resolution BOLD fMRI

PubMed Central

Jung, Youngkyoo; Samsonov, Alexey A.; Liu, Thomas T.; Buracas, Giedrius T.

2012-01-01

Growing demand for high spatial resolution BOLD functional MRI faces a challenge of the spatial resolution vs. coverage or temporal resolution tradeoff, which can be addressed by methods that afford increased acquisition efficiency. Spiral acquisition trajectories have been shown to be superior to currently prevalent echo-planar imaging in terms of acquisition efficiency, and high spatial resolution can be achieved by employing multiple-shot spiral acquisition. The interleaved spiral in-out trajectory is preferred over spiral-in due to increased BOLD signal CNR and higher acquisition efficiency than that of spiral-out or non-interleaved spiral in/out trajectories (1), but to date applicability of the multi-shot interleaved spiral in-out for high spatial resolution imaging has not been studied. Herein we propose multi-shot interleaved spiral in-out acquisition and investigate its applicability for high spatial resolution BOLD fMRI. Images reconstructed from interleaved spiral-in and -out trajectories possess artifacts caused by differences in T2* decay, off-resonance and k-space errors associated with the two trajectories. We analyze the associated errors and demonstrate that application of conjugate phase reconstruction and spectral filtering can substantially mitigate these image artifacts. After applying these processing steps, the multishot interleaved spiral in-out pulse sequence yields high BOLD CNR images at in-plane resolution below 1x1 mm while preserving acceptable temporal resolution (4 s) and brain coverage (15 slices of 2 mm thickness). Moreover, this method yields sufficient BOLD CNR at 1.5 mm isotropic resolution for detection of activation in hippocampus associated with cognitive tasks (Stern memory task). The multi-shot interleaved spiral in-out acquisition is a promising technique for high spatial resolution BOLD fMRI applications. PMID:23023395

High-order multilayer coated blazed gratings for high resolution soft x-ray spectroscopy

DOE PAGES

Voronov, Dmitriy L.; Goray, Leonid I.; Warwick, Tony; ...

2015-02-17

A grand challenge in soft x-ray spectroscopy is to drive the resolving power of monochromators and spectrometers from the 10 4 achieved routinely today to well above 10 5. This need is driven mainly by the requirements of a new technique that is set to have enormous impact in condensed matter physics, Resonant Inelastic X-ray Scattering (RIXS). Unlike x-ray absorption spectroscopy, RIXS is not limited by an energy resolution dictated by the core-hole lifetime in the excitation process. Using much higher resolving power than used for normal x-ray absorption spectroscopy enables access to the energy scale of soft excitations inmore » matter. These excitations such as magnons and phonons drive the collective phenomena seen in correlated electronic materials such as high temperature superconductors. RIXS opens a new path to study these excitations at a level of detail not formerly possible. However, as the process involves resonant excitation at an energy of around 1 keV, and the energy scale of the excitations one would like to see are at the meV level, to fully utilize the technique requires the development of monochromators and spectrometers with one to two orders of magnitude higher energy resolution than has been conventionally possible. Here we investigate the detailed diffraction characteristics of multilayer blazed gratings. These elements offer potentially revolutionary performance as the dispersive element in ultra-high resolution x-ray spectroscopy. In doing so, we have established a roadmap for the complete optimization of the grating design. Traditionally 1st order gratings are used in the soft x-ray region, but we show that as in the optical domain, one can work in very high spectral orders and thus dramatically improve resolution without significant loss in efficiency.« less

Re-assessing Present Day Global Mass Transport and Glacial Isostatic Adjustment From a Data Driven Approach

NASA Astrophysics Data System (ADS)

Wu, X.; Jiang, Y.; Simonsen, S.; van den Broeke, M. R.; Ligtenberg, S.; Kuipers Munneke, P.; van der Wal, W.; Vermeersen, B. L. A.

2017-12-01

Determining present-day mass transport (PDMT) is complicated by the fact that most observations contain signals from both present day ice melting and Glacial Isostatic Adjustment (GIA). Despite decades of progress in geodynamic modeling and new observations, significant uncertainties remain in both. The key to separate present-day ice mass change and signals from GIA is to include data of different physical characteristics. We designed an approach to separate PDMT and GIA signatures by estimating them simultaneously using globally distributed interdisciplinary data with distinct physical information and a dynamically constructed a priori GIA model. We conducted a high-resolution global reappraisal of present-day ice mass balance with focus on Earth's polar regions and its contribution to global sea-level rise using a combination of ICESat, GRACE gravity, surface geodetic velocity data, and an ocean bottom pressure model. Adding ice altimetry supplies critically needed dual data types over the interiors of ice covered regions to enhance separation of PDMT and GIA signatures, and achieve half an order of magnitude expected higher accuracies for GIA and consequently ice mass balance estimates. The global data based approach can adequately address issues of PDMT and GIA induced geocenter motion and long-wavelength signatures important for large areas such as Antarctica and global mean sea level. In conjunction with the dense altimetry data, we solved for PDMT coefficients up to degree and order 180 by using a higher-resolution GRACE data set, and a high-resolution a priori PDMT model that includes detailed geographic boundaries. The high-resolution approach solves the problem of multiple resolutions in various data types, greatly reduces aliased errors from a low-degree truncation, and at the same time, enhances separation of signatures from adjacent regions such as Greenland and Canadian Arctic territories.

Alpha image reconstruction (AIR): A new iterative CT image reconstruction approach using voxel-wise alpha blending

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hofmann, Christian; Sawall, Stefan; Knaup, Michael

2014-06-15

Purpose: Iterative image reconstruction gains more and more interest in clinical routine, as it promises to reduce image noise (and thereby patient dose), to reduce artifacts, or to improve spatial resolution. Among vendors and researchers, however, there is no consensus of how to best achieve these aims. The general approach is to incorporatea priori knowledge into iterative image reconstruction, for example, by adding additional constraints to the cost function, which penalize variations between neighboring voxels. However, this approach to regularization in general poses a resolution noise trade-off because the stronger the regularization, and thus the noise reduction, the stronger themore » loss of spatial resolution and thus loss of anatomical detail. The authors propose a method which tries to improve this trade-off. The proposed reconstruction algorithm is called alpha image reconstruction (AIR). One starts with generating basis images, which emphasize certain desired image properties, like high resolution or low noise. The AIR algorithm reconstructs voxel-specific weighting coefficients that are applied to combine the basis images. By combining the desired properties of each basis image, one can generate an image with lower noise and maintained high contrast resolution thus improving the resolution noise trade-off. Methods: All simulations and reconstructions are performed in native fan-beam geometry. A water phantom with resolution bar patterns and low contrast disks is simulated. A filtered backprojection (FBP) reconstruction with a Ram-Lak kernel is used as a reference reconstruction. The results of AIR are compared against the FBP results and against a penalized weighted least squares reconstruction which uses total variation as regularization. The simulations are based on the geometry of the Siemens Somatom Definition Flash scanner. To quantitatively assess image quality, the authors analyze line profiles through resolution patterns to define a contrast factor for contrast-resolution plots. Furthermore, the authors calculate the contrast-to-noise ratio with the low contrast disks and the authors compare the agreement of the reconstructions with the ground truth by calculating the normalized cross-correlation and the root-mean-square deviation. To evaluate the clinical performance of the proposed method, the authors reconstruct patient data acquired with a Somatom Definition Flash dual source CT scanner (Siemens Healthcare, Forchheim, Germany). Results: The results of the simulation study show that among the compared algorithms AIR achieves the highest resolution and the highest agreement with the ground truth. Compared to the reference FBP reconstruction AIR is able to reduce the relative pixel noise by up to 50% and at the same time achieve a higher resolution by maintaining the edge information from the basis images. These results can be confirmed with the patient data. Conclusions: To evaluate the AIR algorithm simulated and measured patient data of a state-of-the-art clinical CT system were processed. It is shown, that generating CT images through the reconstruction of weighting coefficients has the potential to improve the resolution noise trade-off and thus to improve the dose usage in clinical CT.« less

Mechanisms of time-based figure-ground segregation.

PubMed

Kandil, Farid I; Fahle, Manfred

2003-11-01

Figure-ground segregation can rely on purely temporal information, that is, on short temporal delays between positional changes of elements in figure and ground (Kandil, F.I. & Fahle, M. (2001) Eur. J. Neurosci., 13, 2004-2008). Here, we investigate the underlying mechanisms by measuring temporal segregation thresholds for various kinds of motion cues. Segregation can rely on monocular first-order motion (based on luminance modulation) and second-order motion cues (contrast modulation) with a high temporal resolution of approximately 20 ms. The mechanism can also use isoluminant motion with a reduced temporal resolution of 60 ms. Figure-ground segregation can be achieved even at presentation frequencies too high for human subjects to inspect successive frames individually. In contrast, when stimuli are presented dichoptically, i.e. separately to both eyes, subjects are unable to perceive any segregation, irrespective of temporal frequency. We propose that segregation in these displays is detected by a mechanism consisting of at least two stages. On the first level, standard motion or flicker detectors signal local positional changes (flips). On the second level, a segregation mechanism combines the local activities of the low-level detectors with high temporal precision. Our findings suggest that the segregation mechanism can rely on monocular detectors but not on binocular mechanisms. Moreover, the results oppose the idea that segregation in these displays is achieved by motion detectors of a higher order (motion-from-motion), but favour mechanisms sensitive to short temporal delays even without activation of higher-order motion detectors.

Improving the sensitivity of high-frequency subharmonic imaging with coded excitation: A feasibility study

PubMed Central

Shekhar, Himanshu; Doyley, Marvin M.

2012-01-01

Purpose: Subharmonic intravascular ultrasound imaging (S-IVUS) could visualize the adventitial vasa vasorum, but the high pressure threshold required to incite subharmonic behavior in an ultrasound contrast agent will compromise sensitivity—a trait that has hampered the clinical use of S-IVUS. The purpose of this study was to assess the feasibility of using coded-chirp excitations to improve the sensitivity and axial resolution of S-IVUS. Methods: The subharmonic response of Targestar-pTM, a commercial microbubble ultrasound contrast agent (UCA), to coded-chirp (5%–20% fractional bandwidth) pulses and narrowband sine-burst (4% fractional bandwidth) pulses was assessed, first using computer simulations and then experimentally. Rectangular windowed excitation pulses with pulse durations ranging from 0.25 to 3 μs were used in all studies. All experimental studies were performed with a pair of transducers (20 MHz/10 MHz), both with diameter of 6.35 mm and focal length of 50 mm. The size distribution of the UCA was measured with a CasyTM Cell counter. Results: The simulation predicted a pressure threshold that was an order of magnitude higher than that determined experimentally. However, all other predictions were consistent with the experimental observations. It was predicted that: (1) exciting the agent with chirps would produce stronger subharmonic response relative to those produced by sine-bursts; (2) increasing the fractional bandwidth of coded-chirp excitation would increase the sensitivity of subharmonic imaging; and (3) coded-chirp would increase axial resolution. The experimental results revealed that subharmonic-to-fundamental ratios obtained with chirps were 5.7 dB higher than those produced with sine-bursts of similar duration. The axial resolution achieved with 20% fractional bandwidth chirps was approximately twice that achieved with 4% fractional bandwidth sine-bursts. Conclusions: The coded-chirp method is a suitable excitation strategy for subharmonic IVUS imaging. At the 20 MHz transmission frequency and 20% fractional bandwidth, coded-chirp excitation appears to represent the ideal tradeoff between subharmonic strength and axial resolution. PMID:22482626

Pushing the limits of spatial resolution with the Kuiper Airborne observatory

NASA Technical Reports Server (NTRS)

Lester, Daniel

1994-01-01

The study of astronomical objects at high spatial resolution in the far-IR is one of the most serious limitations to our work at these wavelengths, which carry information about the luminosity of dusty and obscured sources. At IR wavelengths shorter than 30 microns, ground based telescopes with large apertures at superb sites achieve diffraction-limited performance close to the seeing limit in the optical. At millimeter wavelengths, ground based interferometers achieve resolution that is close to this. The inaccessibility of the far-IR from the ground makes it difficult, however, to achieve complementary resolution in the far-IR. The 1983 IRAS survey, while extraordinarily sensitive, provides us with a sky map at a spatial resolution that is limited by detector size on a spatial scale that is far larger than that available in other wavelengths on the ground. The survey resolution is of order 4 min in the 100 micron bandpass, and 2 min at 60 microns (IRAS Explanatory Supplement, 1988). Information on a scale of 1' is available on some sources from the CPC. Deconvolution and image resolution using this database is one of the subjects of this workshop.

Human brain diffusion tensor imaging at submillimeter isotropic resolution on a 3 Tesla clinical MRI scanner

PubMed Central

Chang, Hing-Chiu; Sundman, Mark; Petit, Laurent; Guhaniyogi, Shayan; Chu, Mei-Lan; Petty, Christopher; Song, Allen W.; Chen, Nan-kuei

2015-01-01

The advantages of high-resolution diffusion tensor imaging (DTI) have been demonstrated in a recent post-mortem human brain study (Miller et al., NeuroImage 2011;57(1):167–181), showing that white matter fiber tracts can be much more accurately detected in data at submillimeter isotropic resolution. To our knowledge, in vivo human brain DTI at submillimeter isotropic resolution has not been routinely achieved yet because of the difficulty in simultaneously achieving high resolution and high signal-to-noise ratio (SNR) in DTI scans. Here we report a 3D multi-slab interleaved EPI acquisition integrated with multiplexed sensitivity encoded (MUSE) reconstruction, to achieve high-quality, high-SNR and submillimeter isotropic resolution (0.85 × 0.85 × 0.85 mm3) in vivo human brain DTI on a 3 Tesla clinical MRI scanner. In agreement with the previously reported post-mortem human brain DTI study, our in vivo data show that the structural connectivity networks of human brains can be mapped more accurately and completely with high-resolution DTI as compared with conventional DTI (e.g., 2 × 2 × 2 mm3). PMID:26072250

Swept Field Laser Confocal Microscopy for Enhanced Spatial and Temporal Resolution in Live-Cell Imaging

PubMed Central

Castellano-Muñoz, Manuel; Peng, Anthony Wei; Salles, Felipe T.; Ricci, Anthony J.

2013-01-01

Confocal fluorescence microscopy is a broadly used imaging technique that enhances the signal-to-noise ratio by removing out of focal plane fluorescence. Confocal microscopes come with a variety of modifications depending on the particular experimental goals. Microscopes, illumination pathways, and light collection were originally focused upon obtaining the highest resolution image possible, typically on fixed tissue. More recently, live-cell confocal imaging has gained importance. Since measured signals are often rapid or transient, thus requiring higher sampling rates, specializations are included to enhance spatial and temporal resolution while maintaining tissue viability. Thus, a balance between image quality, temporal resolution, and tissue viability is needed. A subtype of confocal imaging, termed swept field confocal (SFC) microscopy, can image live cells at high rates while maintaining confocality. SFC systems can use a pinhole array to obtain high spatial resolution, similar to spinning disc systems. In addition, SFC imaging can achieve faster rates by using a slit to sweep the light across the entire image plane, thus requiring a single scan to generate an image. Coupled to a high-speed charge-coupled device camera and a laser illumination source, images can be obtained at greater than 1,000 frames per second while maintaining confocality. PMID:22831554

Compliance with recommendations for tympanostomy tube follow-up: patient characteristics.

PubMed

Kao, Richard; Kirse, Daniel J; Evans, Adele K

2014-09-01

(1) To determine the percentage of otherwise healthy patients achieving "graduation," or postoperative compliance achieving complete problem resolution and discharge from the otolaryngologist's care, after tympanostomy tube placement. (2) To analyze follow-up behaviors and patient characteristics influencing the likelihood of graduation. Retrospective cohort study. Tertiary care hospital. Analysis of details of tympanostomy tube placements performed from 2004 to 2011 by 2 pediatric otolaryngologists for children aged 0 to 18 years. Exclusion criteria were clearly defined craniofacial anomalies, cleft palates, and other ongoing postoperative care. The remaining study subjects were categorized into 3 groups. Graduation (GRAD) subjects achieved discharge from care with "follow-up pro re nata" status. LOST<2Y subjects had not attended scheduled follow-up in <2 years. LOST≥2Y subjects had no follow-up in ≥2 years. A total of 1454 pediatric subjects were included. GRADs constituted only 25.6% of the subject pool; 22.1% were LOST<2Y, and 52.3% were LOST≥2Y. Statistically significant factors in achieving graduation were total number of follow-up visits, total duration of follow-up, compliance with first postoperative visit, patient age, insurance type, and distance between home and practice. Rate of graduation, or postoperative compliance achieving complete problem resolution, of otherwise healthy tympanostomy tube patients is low despite perioperative discussions of the importance of proper follow-up. Higher graduation rates are associated with increasing number of follow-up visits and duration, younger patient age, private insurance, and proximity to the practice. Compliance with attending the first postoperative visit may be an early marker for increased likelihood of graduation. © American Academy of Otolaryngology—Head and Neck Surgery Foundation 2014.

Wavy Architecture Thin-Film Transistor for Ultrahigh Resolution Flexible Displays.

PubMed

Hanna, Amir Nabil; Kutbee, Arwa Talal; Subedi, Ram Chandra; Ooi, Boon; Hussain, Muhammad Mustafa

2018-01-01

A novel wavy-shaped thin-film-transistor (TFT) architecture, capable of achieving 70% higher drive current per unit chip area when compared with planar conventional TFT architectures, is reported for flexible display application. The transistor, due to its atypical architecture, does not alter the turn-on voltage or the OFF current values, leading to higher performance without compromising static power consumption. The concept behind this architecture is expanding the transistor's width vertically through grooved trenches in a structural layer deposited on a flexible substrate. Operation of zinc oxide (ZnO)-based TFTs is shown down to a bending radius of 5 mm with no degradation in the electrical performance or cracks in the gate stack. Finally, flexible low-power LEDs driven by the respective currents of the novel wavy, and conventional coplanar architectures are demonstrated, where the novel architecture is able to drive the LED at 2 × the output power, 3 versus 1.5 mW, which demonstrates the potential use for ultrahigh resolution displays in an area efficient manner. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

GRACE time-variable gravity field recovery using an improved energy balance approach

NASA Astrophysics Data System (ADS)

Shang, Kun; Guo, Junyi; Shum, C. K.; Dai, Chunli; Luo, Jia

2015-12-01

A new approach based on energy conservation principle for satellite gravimetry mission has been developed and yields more accurate estimation of in situ geopotential difference observables using K-band ranging (KBR) measurements from the Gravity Recovery and Climate Experiment (GRACE) twin-satellite mission. This new approach preserves more gravity information sensed by KBR range-rate measurements and reduces orbit error as compared to previous energy balance methods. Results from analysis of 11 yr of GRACE data indicated that the resulting geopotential difference estimates agree well with predicted values from official Level 2 solutions: with much higher correlation at 0.9, as compared to 0.5-0.8 reported by previous published energy balance studies. We demonstrate that our approach produced a comparable time-variable gravity solution with the Level 2 solutions. The regional GRACE temporal gravity solutions over Greenland reveals that a substantially higher temporal resolution is achievable at 10-d sampling as compared to the official monthly solutions, but without the compromise of spatial resolution, nor the need to use regularization or post-processing.

Focal Plane Detectors for the Advanced Gamma-Ray Imaging System (AGIS)

NASA Astrophysics Data System (ADS)

Otte, A. N.; Byrum, K.; Drake, G.; Falcone, A.; Funk, S.; Horan, D.; Mukherjee, R.; Smith, A.; Tajima, H.; Wagner, R. G.; Williams, D. A.

2008-12-01

The Advanced Gamma-Ray Imaging System (AGIS) is a concept for the next generation observatory in ground-based very high energy gamma-ray astronomy. Design goals are ten times better sensitivity, higher angular resolution, and a lower energy threshold than existing Cherenkov telescopes. Simulations show that a substantial improvement in angular resolution may be achieved if the pixel diameter is reduced to the order of 0.05 deg, i.e. two to three times smaller than the pixel diameter of current Cherenkov telescope cameras. At these dimensions, photon detectors with smaller physical dimensions can be attractive alternatives to the classical photomultiplier tube (PMT). Furthermore, the operation of an experiment with the size of AGIS requires photon detectors that are among other things more reliable, more durable, and possibly higher efficiency photon detectors. Alternative photon detectors we are considering for AGIS include both silicon photomultipliers (SiPMs) and multi-anode photomultipliers (MAPMTs). Here we present results from laboratory testing of MAPMTs and SiPMs along with results from the first incorporation of these devices into cameras on test bed Cherenkov telescopes.

Achieving High Resolution Timer Events in Virtualized Environment

PubMed Central

Adamczyk, Blazej; Chydzinski, Andrzej

2015-01-01

Virtual Machine Monitors (VMM) have become popular in different application areas. Some applications may require to generate the timer events with high resolution and precision. This however may be challenging due to the complexity of VMMs. In this paper we focus on the timer functionality provided by five different VMMs—Xen, KVM, Qemu, VirtualBox and VMWare. Firstly, we evaluate resolutions and precisions of their timer events. Apparently, provided resolutions and precisions are far too low for some applications (e.g. networking applications with the quality of service). Then, using Xen virtualization we demonstrate the improved timer design that greatly enhances both the resolution and precision of achieved timer events. PMID:26177366

Two-Dimensional Standing Wave Total Internal Reflection Fluorescence Microscopy: Superresolution Imaging of Single Molecular and Biological Specimens

PubMed Central

Chung, Euiheon; Kim, Daekeun; Cui, Yan; Kim, Yang-Hyo; So, Peter T. C.

2007-01-01

The development of high resolution, high speed imaging techniques allows the study of dynamical processes in biological systems. Lateral resolution improvement of up to a factor of 2 has been achieved using structured illumination. In a total internal reflection fluorescence microscope, an evanescence excitation field is formed as light is total internally reflected at an interface between a high and a low index medium. The <100 nm penetration depth of evanescence field ensures a thin excitation region resulting in low background fluorescence. We present even higher resolution wide-field biological imaging by use of standing wave total internal reflection fluorescence (SW-TIRF). Evanescent standing wave (SW) illumination is used to generate a sinusoidal high spatial frequency fringe pattern on specimen for lateral resolution enhancement. To prevent thermal drift of the SW, novel detection and estimation of the SW phase with real-time feedback control is devised for the stabilization and control of the fringe phase. SW-TIRF is a wide-field superresolution technique with resolution better than a fifth of emission wavelength or ∼100 nm lateral resolution. We demonstrate the performance of the SW-TIRF microscopy using one- and two-directional SW illumination with a biological sample of cellular actin cytoskeleton of mouse fibroblast cells as well as single semiconductor nanocrystal molecules. The results confirm the superior resolution of SW-TIRF in addition to the merit of a high signal/background ratio from TIRF microscopy. PMID:17483188

High temperature sensing using higher-order-mode rejected sapphire-crystal fiber gratings

NASA Astrophysics Data System (ADS)

Zhan, Chun; Kim, Jae Hun; Lee, Jon; Yin, Stuart; Ruffin, Paul; Luo, Claire

2007-09-01

In this paper, we report the fabrication of higher-order-mode rejected fiber Bragg gratings (FBGs) in sapphire crystal fiber using infrared (IR) femtosecond laser illumination. The grating is tested in high temperature furnace up to 1600 degree Celsius. As sapphire fiber is only available as highly multimode fiber, a scheme to filter out higher order modes in favor for the fundamental mode is theoretically evaluated and experimentally demonstrated. The approach is to use an ultra thin sapphire crystal fiber (60 micron in diameter) to decrease the number of modes. The small diameter fiber also enables bending the fiber to certain radius which is carefully chosen to provide low loss for the fundamental mode LP01 and high loss for the other high-order modes. After bending, less-than-2-nm resonant peak bandwidth is achieved. The grating spectrum is improved, and higher resolution sensing measurement can be achieved. This mode filtering method is very easy to implement. Furthermore, the sapphire fiber is sealed with hi-purity alumina ceramic cement inside a flexible high temperature titanium tube, and the highly flexible titanium tube offers a robust packaging to sapphire fiber. Our high temperature sapphire grating sensor is very promising in extremely high temperature sensing application.

Improving Secondary Ion Mass Spectrometry Image Quality with Image Fusion

PubMed Central

Tarolli, Jay G.; Jackson, Lauren M.; Winograd, Nicholas

2014-01-01

The spatial resolution of chemical images acquired with cluster secondary ion mass spectrometry (SIMS) is limited not only by the size of the probe utilized to create the images, but also by detection sensitivity. As the probe size is reduced to below 1 µm, for example, a low signal in each pixel limits lateral resolution due to counting statistics considerations. Although it can be useful to implement numerical methods to mitigate this problem, here we investigate the use of image fusion to combine information from scanning electron microscope (SEM) data with chemically resolved SIMS images. The advantage of this approach is that the higher intensity and, hence, spatial resolution of the electron images can help to improve the quality of the SIMS images without sacrificing chemical specificity. Using a pan-sharpening algorithm, the method is illustrated using synthetic data, experimental data acquired from a metallic grid sample, and experimental data acquired from a lawn of algae cells. The results show that up to an order of magnitude increase in spatial resolution is possible to achieve. A cross-correlation metric is utilized for evaluating the reliability of the procedure. PMID:24912432

Development of digital reconstructed radiography software at new treatment facility for carbon-ion beam scanning of National Institute of Radiological Sciences.

PubMed

Mori, Shinichiro; Inaniwa, Taku; Kumagai, Motoki; Kuwae, Tsunekazu; Matsuzaki, Yuka; Furukawa, Takuji; Shirai, Toshiyuki; Noda, Koji

2012-06-01

To increase the accuracy of carbon ion beam scanning therapy, we have developed a graphical user interface-based digitally-reconstructed radiograph (DRR) software system for use in routine clinical practice at our center. The DRR software is used in particular scenarios in the new treatment facility to achieve the same level of geometrical accuracy at the treatment as at the imaging session. DRR calculation is implemented simply as the summation of CT image voxel values along the X-ray projection ray. Since we implemented graphics processing unit-based computation, the DRR images are calculated with a speed sufficient for the particular clinical practice requirements. Since high spatial resolution flat panel detector (FPD) images should be registered to the reference DRR images in patient setup process in any scenarios, the DRR images also needs higher spatial resolution close to that of FPD images. To overcome the limitation of the CT spatial resolution imposed by the CT voxel size, we applied image processing to improve the calculated DRR spatial resolution. The DRR software introduced here enabled patient positioning with sufficient accuracy for the implementation of carbon-ion beam scanning therapy at our center.

Influence of Molecular Resolution on Sequence-Based Discovery of Ecological Diversity among Synechococcus Populations in an Alkaline Siliceous Hot Spring Microbial Mat ▿ †

PubMed Central

Melendrez, Melanie C.; Lange, Rachel K.; Cohan, Frederick M.; Ward, David M.

2011-01-01

Previous research has shown that sequences of 16S rRNA genes and 16S-23S rRNA internal transcribed spacer regions may not have enough genetic resolution to define all ecologically distinct Synechococcus populations (ecotypes) inhabiting alkaline, siliceous hot spring microbial mats. To achieve higher molecular resolution, we studied sequence variation in three protein-encoding loci sampled by PCR from 60°C and 65°C sites in the Mushroom Spring mat (Yellowstone National Park, WY). Sequences were analyzed using the ecotype simulation (ES) and AdaptML algorithms to identify putative ecotypes. Between 4 and 14 times more putative ecotypes were predicted from variation in protein-encoding locus sequences than from variation in 16S rRNA and 16S-23S rRNA internal transcribed spacer sequences. The number of putative ecotypes predicted depended on the number of sequences sampled and the molecular resolution of the locus. Chao estimates of diversity indicated that few rare ecotypes were missed. Many ecotypes hypothesized by sequence analyses were different in their habitat specificities, suggesting different adaptations to temperature or other parameters that vary along the flow channel. PMID:21169433

A robust multi-shot scan strategy for high-resolution diffusion weighted MRI enabled by multiplexed sensitivity-encoding (MUSE)

PubMed Central

Chen, Nan-kuei; Guidon, Arnaud; Chang, Hing-Chiu; Song, Allen W.

2013-01-01

Diffusion weighted magnetic resonance imaging (DWI) data have been mostly acquired with single-shot echo-planar imaging (EPI) to minimize motion induced artifacts. The spatial resolution, however, is inherently limited in single-shot EPI, even when the parallel imaging (usually at an acceleration factor of 2) is incorporated. Multi-shot acquisition strategies could potentially achieve higher spatial resolution and fidelity, but they are generally susceptible to motion-induced phase errors among excitations that are exacerbated by diffusion sensitizing gradients, rendering the reconstructed images unusable. It has been shown that shot-to-shot phase variations may be corrected using navigator echoes, but at the cost of imaging throughput. To address these challenges, a novel and robust multi-shot DWI technique, termed multiplexed sensitivity-encoding (MUSE), is developed here to reliably and inherently correct nonlinear shot-to-shot phase variations without the use of navigator echoes. The performance of the MUSE technique is confirmed experimentally in healthy adult volunteers on 3 Tesla MRI systems. This newly developed technique should prove highly valuable for mapping brain structures and connectivities at high spatial resolution for neuroscience studies. PMID:23370063

On the use of high-resolution topographic data as a proxy for seismic site conditions (VS30)

USGS Publications Warehouse

Allen, T.I.; Wald, D.J.

2009-01-01

An alternative method has recently been proposed for evaluating global seismic site conditions, or the average shear velocity to 30 m depth (VS30), from the Shuttle Radar Topography Mission (SRTM) 30 arcsec digital elevation models (DEMs). The basic premise of the method is that the topographic slope can be used as a reliable proxy for VS30 in the absence of geologically and geotechnically based site-condition maps through correlations between VS30 measurements and topographic gradient. Here we evaluate the use of higher-resolution (3 and 9 arcsec) DEMs to examine whether we are able to resolve VS30 in more detail than can be achieved using the lower-resolution SRTM data. High-quality DEMs at resolutions greater than 30 arcsec are not uniformly available at the global scale. However, in many regions where such data exist, they may be employed to resolve finer-scale variations in topographic gradient, and consequently, VS30. We use the U.S. Geological Survey Earth Resources Observation and Science (EROS) Data Center's National Elevation Dataset (NED) to investigate the use of high-resolution DEMs for estimating VS30 in several regions across the United States, including the San Francisco Bay area in California, Los Angeles, California, and St. Louis, Missouri. We compare these results with an example from Taipei, Taiwan, that uses 9 arcsec SRTM data, which are globally available. The use of higher-resolution NED data recovers finer-scale variations in topographic gradient, which better correlate to geological and geomorphic features, in particular, at the transition between hills and basins, warranting their use over 30 arcsec SRTM data where available. However, statistical analyses indicate little to no improvement over lower-resolution topography when compared to VS30 measurements, suggesting that some topographic smoothing may provide more stable VS30 estimates. Furthermore, we find that elevation variability in canopy-based SRTM measurements at resolutions greater than 30 arcsec are too large to resolve reliable slopes, particularly in low-gradient sedimentary basins.

Near-infrared high-resolution real-time omnidirectional imaging platform for drone detection

NASA Astrophysics Data System (ADS)

Popovic, Vladan; Ott, Beat; Wellig, Peter; Leblebici, Yusuf

2016-10-01

Recent technological advancements in hardware systems have made higher quality cameras. State of the art panoramic systems use them to produce videos with a resolution of 9000 x 2400 pixels at a rate of 30 frames per second (fps).1 Many modern applications use object tracking to determine the speed and the path taken by each object moving through a scene. The detection requires detailed pixel analysis between two frames. In fields like surveillance systems or crowd analysis, this must be achieved in real time.2 In this paper, we focus on the system-level design of multi-camera sensor acquiring near-infrared (NIR) spectrum and its ability to detect mini-UAVs in a representative rural Swiss environment. The presented results show the UAV detection from the trial that we conducted during a field trial in August 2015.

Human iris three-dimensional imaging at micron resolution by a micro-plenoptic camera

PubMed Central

Chen, Hao; Woodward, Maria A.; Burke, David T.; Jeganathan, V. Swetha E.; Demirci, Hakan; Sick, Volker

2017-01-01

A micro-plenoptic system was designed to capture the three-dimensional (3D) topography of the anterior iris surface by simple single-shot imaging. Within a depth-of-field of 2.4 mm, depth resolution of 10 µm can be achieved with accuracy (systematic errors) and precision (random errors) below 20%. We demonstrated the application of our micro-plenoptic imaging system on two healthy irides, an iris with naevi, and an iris with melanoma. The ridges and folds, with height differences of 10~80 µm, on the healthy irides can be effectively captured. The front surface on the iris naevi was flat, and the iris melanoma was 50 ± 10 µm higher than the surrounding iris. The micro-plenoptic imaging system has great potential to be utilized for iris disease diagnosis and continuing, simple monitoring. PMID:29082081

Human iris three-dimensional imaging at micron resolution by a micro-plenoptic camera.

PubMed

Chen, Hao; Woodward, Maria A; Burke, David T; Jeganathan, V Swetha E; Demirci, Hakan; Sick, Volker

2017-10-01

A micro-plenoptic system was designed to capture the three-dimensional (3D) topography of the anterior iris surface by simple single-shot imaging. Within a depth-of-field of 2.4 mm, depth resolution of 10 µm can be achieved with accuracy (systematic errors) and precision (random errors) below 20%. We demonstrated the application of our micro-plenoptic imaging system on two healthy irides, an iris with naevi, and an iris with melanoma. The ridges and folds, with height differences of 10~80 µm, on the healthy irides can be effectively captured. The front surface on the iris naevi was flat, and the iris melanoma was 50 ± 10 µm higher than the surrounding iris. The micro-plenoptic imaging system has great potential to be utilized for iris disease diagnosis and continuing, simple monitoring.

Trapping and spectroscopy of hydrogen

NASA Astrophysics Data System (ADS)

Cesar, Claudio Lenz

1997-08-01

I review the results and techniques used by the MIT H↑ group to achieve a fractional resolution of 2 parts in 1012 in the 1S-2S transition in hydrogen [Cesar, D. Fried, T. Killian, A. Polcyn, J. Sandberg, I.A. Yu, T. Greytak, D. Kleppner and J. Doyle, Two-photon spectroscopy of trapped atomic hydrogen, Phys. Rev. Lett. 77 (1996) 255.] With some improvements, this system should deliver 100 times higher resolution with an improved signal count rate getting us closer to an old advertised goal of a precision of 1 part in 1018. While these developments are very important for the proposed test of the CPT theorem through the comparison with anti-hydrogen, some of the techniques used with hydrogen are not applicable to anti-hydrogen and I discuss some difficulties and alternatives for the trapping and spectroscopy of anti-hydrogen.

Ultra-stable sub-meV monochromator for hard X-rays

DOE PAGES

Toellner, T. S.; Collins, J.; Goetze, K.; ...

2015-07-17

A high-resolution silicon monochromator suitable for 21.541 keV synchrotron radiation is presented that produces a bandwidth of 0.27 meV. The operating energy corresponds to a nuclear transition in 151Eu. The first-of-its-kind, fully cryogenic design achieves an energy-alignment stability of 0.017 meV r.m.s. per day, or a 100-fold improvement over other meV-monochromators, and can tolerate higher X-ray power loads than room-temperature designs of comparable resolution. This offers the potential for significantly more accurate measurements of lattice excitation energies using nuclear resonant vibrational spectroscopy if combined with accurate energy calibration using, for example, high-speed Doppler shifting. The design of the monochromator alongmore » with its performance and impact on transmitted beam properties are presented.« less

Wide field of view spectroscopy using solid Fabry-Perot interferometers

NASA Astrophysics Data System (ADS)

Nikoleyczik, Jonathan; Kutyrev, Alexander; Moseley, Harvey; Veilleux, Sylvain

2016-08-01

We present a high resolution spectrometer consisting of dual solid Fabry-Perot Interferometers (FPI). Each FPI is made of a single piece of L-BBH2 glass which has a high index of refraction n 2.07. Each is then coated with partially reflective mirrors to achieve a spectral resolution of R 30,000. Running the FPIs in tandem reduces the overlapping orders and allows for a much wider free spectral range and higher contrast. Tuning of the FPIs is achieved by adjusting the temperature and thus changing the FPI gap and the refractive index of the material. The spectrometer then moves spatially in order to get spectral information at every point in the field of view. We select spectral lines for further analysis and create maps of the line depths across the field. Using this technique we are able to measure the fluorescence of chlorophyll in plants and observe zodiacal light. In the chlorophyll analysis we are able to detect chlorophyll fluorescence using the line depth in a plant using the sky as a reference solar spectrum. This instrument has possible applications in either a cubesat or aerial observations to measure bulk plant activity over large areas.

Data fusion and photometric restoration

NASA Astrophysics Data System (ADS)

Pirzkal, Norbert; Hook, Richard N.

2001-11-01

The current generation of 8-10m optical ground-based telescopes have a symbiotic relationship with space telescopes. For direct imaging in the optical the former can collect photons relatively cheaply but the latter can still achieve, even in the era of adaptive optics, significantly higher spatial resolution, point-spread function stability and astrometric fidelity over fields of a few arcminutes. The large archives of HST imaging already in place, when combined with the ease of access to ground-based data afforded by the virtual observatory currently under development, will make space-ground data fusion a powerful tool for the future. We describe a photometric image restoration method that we have developed which allows the efficient and accurate use of high-resolution space imaging of crowded fields to extract high quality photometry from very crowded ground-based images. We illustrate the method using HST and ESO VLT/FORS imaging of a globular cluster and demonstrate quantitatively the photometric measurements quality that can achieved using the data fusion approach instead of just using data from just one telescope. This method can handle most of the common difficulties encountered when attempting this problem such as determining the geometric mapping to the requisite precision, deriving the PSF and the background.

SRRF: Universal live-cell super-resolution microscopy.

PubMed

Culley, Siân; Tosheva, Kalina L; Matos Pereira, Pedro; Henriques, Ricardo

2018-08-01

Super-resolution microscopy techniques break the diffraction limit of conventional optical microscopy to achieve resolutions approaching tens of nanometres. The major advantage of such techniques is that they provide resolutions close to those obtainable with electron microscopy while maintaining the benefits of light microscopy such as a wide palette of high specificity molecular labels, straightforward sample preparation and live-cell compatibility. Despite this, the application of super-resolution microscopy to dynamic, living samples has thus far been limited and often requires specialised, complex hardware. Here we demonstrate how a novel analytical approach, Super-Resolution Radial Fluctuations (SRRF), is able to make live-cell super-resolution microscopy accessible to a wider range of researchers. We show its applicability to live samples expressing GFP using commercial confocal as well as laser- and LED-based widefield microscopes, with the latter achieving long-term timelapse imaging with minimal photobleaching. Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.

Second generation stationary digital breast tomosynthesis system with faster scan time and wider angular span.

PubMed

Calliste, Jabari; Wu, Gongting; Laganis, Philip E; Spronk, Derrek; Jafari, Houman; Olson, Kyle; Gao, Bo; Lee, Yueh Z; Zhou, Otto; Lu, Jianping

2017-09-01

The aim of this study was to characterize a new generation stationary digital breast tomosynthesis system with higher tube flux and increased angular span over a first generation system. The linear CNT x-ray source was designed, built, and evaluated to determine its performance parameters. The second generation system was then constructed using the CNT x-ray source and a Hologic gantry. Upon construction, test objects and phantoms were used to characterize system resolution as measured by the modulation transfer function (MTF), and artifact spread function (ASF). The results indicated that the linear CNT x-ray source was capable of stable operation at a tube potential of 49 kVp, and measured focal spot sizes showed source-to-source consistency with a nominal focal spot size of 1.1 mm. After construction, the second generation (Gen 2) system exhibited entrance surface air kerma rates two times greater the previous s-DBT system. System in-plane resolution as measured by the MTF is 7.7 cycles/mm, compared to 6.7 cycles/mm for the Gen 1 system. As expected, an increase in the z-axis depth resolution was observed, with a decrease in the ASF from 4.30 mm to 2.35 mm moving from the Gen 1 system to the Gen 2 system as result of an increased angular span. The results indicate that the Gen 2 stationary digital breast tomosynthesis system, which has a larger angular span, increased entrance surface air kerma, and faster image acquisition time over the Gen 1 s-DBT system, results in higher resolution images. With the detector operating at full resolution, the Gen 2 s-DBT system can achieve an in-plane resolution of 7.7 cycles per mm, which is better than the current commercial DBT systems today, and may potentially result in better patient diagnosis. © 2017 American Association of Physicists in Medicine.

Discrimination of natural and cultivated vegetation using Thematic Mapper spectral data

NASA Technical Reports Server (NTRS)

Degloria, Stephen D.; Bernstein, Ralph; Dizenzo, Silvano

1986-01-01

The availability of high quality spectral data from the current suite of earth observation satellite systems offers significant improvements in the ability to survey and monitor food and fiber production on both a local and global basis. Current research results indicate that Landsat TM data when used in either digital or analog formats achieve higher land-cover classification accuracies than MSS data using either comparable or improved spectral bands and spatial resolution. A review of these quantitative results is presented for both natural and cultivated vegetation.

Design, building and test of one prototype and four final position sensor assemblies: Hall effect position sensors

NASA Technical Reports Server (NTRS)

1976-01-01

This report covers the development of a three channel Hall effect position sensing system for the commutation of a three phase dc torquer motor. The effort consisted of the evaluation, modification and re-packaging of a commercial position sensor and the design of a target configuration unique to this application. The resulting design meets the contract requirements and, furthermore, the test results indicate not only the practicality and versatility of the design, but also that there may be higher limits of resolution and accuracy achievable.

Supercomputer algorithms for efficient linear octree encoding of three-dimensional brain images.

PubMed

Berger, S B; Reis, D J

1995-02-01

We designed and implemented algorithms for three-dimensional (3-D) reconstruction of brain images from serial sections using two important supercomputer architectures, vector and parallel. These architectures were represented by the Cray YMP and Connection Machine CM-2, respectively. The programs operated on linear octree representations of the brain data sets, and achieved 500-800 times acceleration when compared with a conventional laboratory workstation. As the need for higher resolution data sets increases, supercomputer algorithms may offer a means of performing 3-D reconstruction well above current experimental limits.

Recycling isotachophoresis - A novel approach to preparative protein fractionation

NASA Technical Reports Server (NTRS)

Sloan, Jeffrey E.; Thormann, Wolfgang; Bier, Milan; Twitty, Garland E.; Mosher, Richard A.

1986-01-01

The concept of automated recycling isotachophoresis (RITP) as a purification methodology is discussed, in addition to a description of the apparatus. In the present automated RITP, the computer system follows the achievement of steady state using arrays of universal and specific sensors, monitors the position of the front edge of the zone structure, activates the counterflow if the leading boundary passes a specified position along the separation axis, or changes the applied current, accordingly. The system demonstrates high resolution, in addition to higher processing rates than are possible in zone electrophoresis or isoelectric focusing.

Air Pollution Measurements by Citizen Scientists and NASA Satellites: Data Integration and Analysis

NASA Astrophysics Data System (ADS)

Gupta, P.; Maibach, J.; Levy, R. C.; Doraiswamy, P.; Pikelnaya, O.; Feenstra, B.; Polidori, A.

2017-12-01

PM2.5, or fine particulate matter, is a category of air pollutant consisting of solid particles with effective aerodynamic diameter of less than 2.5 microns. These particles are hazardous to human health, as their small size allows them to penetrate deep into the lungs. Since the late 1990's, the US Environmental Protection Agency has been monitoring PM2.5 using a network of ground-level sensors. Due to cost and space restrictions, the EPA monitoring network remains spatially sparse. That is, while the network spans the extent of the US, the distance between sensors is large enough that significant spatial variation in PM concentration can go undetected. To increase the spatial resolution of monitoring, previous studies have used satellite data to estimate ground-level PM concentrations. From imagery, one can create a measure of haziness due to aerosols, called aerosol optical depth (AOD), which then can be used to estimate PM concentrations using statistical and physical modeling. Additionally, previous research has identified a number of meteorological variables, such as relative humidity and mixing height, which aide in estimating PM concentrations from AOD. Although the high spatial resolution of satellite data is valuable alone for forecasting air quality, higher resolution ground-level data is needed to effectively study the relationship between PM2.5 concentrations and AOD. To this end, we discuss a citizen-science PM monitoring network deployed in California. Using low-cost PM sensors, this network achieves higher spatial resolution. We additionally discuss a software pipeline for integrating resulting PM measurements with satellite data, as well as initial data analysis.

Luma-chroma space filter design for subpixel-based monochrome image downsampling.

PubMed

Fang, Lu; Au, Oscar C; Cheung, Ngai-Man; Katsaggelos, Aggelos K; Li, Houqiang; Zou, Feng

2013-10-01

In general, subpixel-based downsampling can achieve higher apparent resolution of the down-sampled images on LCD or OLED displays than pixel-based downsampling. With the frequency domain analysis of subpixel-based downsampling, we discover special characteristics of the luma-chroma color transform choice for monochrome images. With these, we model the anti-aliasing filter design for subpixel-based monochrome image downsampling as a human visual system-based optimization problem with a two-term cost function and obtain a closed-form solution. One cost term measures the luminance distortion and the other term measures the chrominance aliasing in our chosen luma-chroma space. Simulation results suggest that the proposed method can achieve sharper down-sampled gray/font images compared with conventional pixel and subpixel-based methods, without noticeable color fringing artifacts.

A phase-based stereo vision system-on-a-chip.

PubMed

Díaz, Javier; Ros, Eduardo; Sabatini, Silvio P; Solari, Fabio; Mota, Sonia

2007-02-01

A simple and fast technique for depth estimation based on phase measurement has been adopted for the implementation of a real-time stereo system with sub-pixel resolution on an FPGA device. The technique avoids the attendant problem of phase warping. The designed system takes full advantage of the inherent processing parallelism and segmentation capabilities of FPGA devices to achieve a computation speed of 65megapixels/s, which can be arranged with a customized frame-grabber module to process 211frames/s at a size of 640x480 pixels. The processing speed achieved is higher than conventional camera frame rates, thus allowing the system to extract multiple estimations and be used as a platform to evaluate integration schemes of a population of neurons without increasing hardware resource demands.

Optical frequency switching scheme for a high-speed broadband THz measurement system based on the photomixing technique.

PubMed

Song, Hajun; Hwang, Sejin; Song, Jong-In

2017-05-15

This study presents an optical frequency switching scheme for a high-speed broadband terahertz (THz) measurement system based on the photomixing technique. The proposed system can achieve high-speed broadband THz measurements using narrow optical frequency scanning of a tunable laser source combined with a wavelength-switchable laser source. In addition, this scheme can provide a larger output power of an individual THz signal compared with that of a multi-mode THz signal generated by multiple CW laser sources. A swept-source THz tomography system implemented with a two-channel wavelength-switchable laser source achieves a reduced time for acquisition of a point spread function and a higher depth resolution in the same amount of measurement time compared with a system with a single optical source.

Design challenges of EO polymer based leaky waveguide deflector for 40 Gs/s all-optical analog-to-digital converters

NASA Astrophysics Data System (ADS)

Hadjloum, Massinissa; El Gibari, Mohammed; Li, Hongwu; Daryoush, Afshin S.

2016-08-01

Design challenges and performance optimization of an all-optical analog-to-digital converter (AOADC) is presented here. The paper addresses both microwave and optical design of a leaky waveguide optical deflector using electro-optic (E-O) polymer. The optical deflector converts magnitude variation of the applied RF voltage into variation of deflection angle out of a leaky waveguide optical beam using the linear E-O effect (Pockels effect) as part of the E-O polymer based optical waveguide. This variation of deflection angle as result of the applied RF signal is then quantized using optical windows followed by an array of high-speed photodetectors. We optimized the leakage coefficient of the leaky waveguide and its physical length to achieve the best trade-off between bandwidth and the deflected optical beam resolution, by improving the phase velocity matching between lightwave and microwave on one hand and using pre-emphasis technique to compensate for the RF signal attenuation on the other hand. In addition, for ease of access from both optical and RF perspective, a via-hole less broad bandwidth transition is designed between coplanar pads and coupled microstrip (CPW-CMS) driving electrodes. With the best reported E-O coefficient of 350 pm/V, the designed E-O deflector should allow an AOADC operating over 44 giga-samples-per-seconds with an estimated effective resolution of 6.5 bits on RF signals with Nyquist bandwidth of 22 GHz. The overall DC power consumption of all components used in this AOADC is of order of 4 W and is dominated by power consumption in the power amplifier to generate a 20 V RF voltage in 50 Ohm system. A higher sampling rate can be achieved at similar bits of resolution by interleaving a number of this elementary AOADC at the expense of a higher power consumption.

Inter-comparison between AIRS and IASI through Retrieved Parameters

NASA Technical Reports Server (NTRS)

Zhou, Daniel K.; Larar, Allen M.; Smith, William L.; Taylor, Jonathan P.; Schluessel, Peter; Strow, L. Larrabee; Mango, Steve

2008-01-01

A State-of-the-art retrieval algorithm dealing with all-weather conditions has been applied to satellite/aircraft instruments retrieving cloud/surface and atmospheric conditions. High quality retrievals have been achieved from IASI data. Surface, cloud, and atmospheric structure and variation are well captured by IASI measurements and/or retrievals. The same retrieval algorithm is also applied to AIRS for retrieval inter-comparison. Both AIRS and IASI have a similar FOV size but AIRS has a higher horizontal resolution. AIRS data can be interpolated to IASI horizontal resolution for inter-comparison at the same geophysical locations, however a temporal variation between AIRS and IASI observations need to be considered. JAIVEx has employed aircraft to obtain the atmospheric variation filling the temporal gap between two satellites. First results show that both AIRS and IASI have a very similar vertical resolving power, atmospheric conditions are well captured by both instruments, and radiances are well calibrated. AIRS data shown in retrievals (e.g., surface emissivity and moisture) have a relatively higher noise level. Since the this type of retrieval is very sensitive to its radiance quality, retrieval products inter-comparison is an effective way to identify/compare their radiance quality, in terms of a combination of spectral resolution and noise level, and to assess instrument performance. Additional validation analyses are needed to provide more-definitive conclusions.

Optical Super-Resolution by High-Index Liquid-Immersed Microspheres

DTIC Science & Technology

2012-01-01

the BD without liquid can be achieved using microspheres with small-to-moderate index of refraction such as borosilicate glass (n 1.47), soda lime ...titanate glass microspheres with diameters (D) in the range 2–220 lm and with high refractive index (n 1.9–2.1) can be used for super-resolution...achieving optical super-resolution. It has been demonstrated10 that silica spheres with refractive index (n) about 1.46 and with diame- ters (D) in the

RadioAstron Maser Observations: a Record in Angular Resolution

NASA Astrophysics Data System (ADS)

Sobolev, A. M.; Shakhvorostova, N. N.; Alakoz, A. V.; Baan, W. A.; RadioAstron Maser Team

2017-06-01

Extremely long baselines of the space-ground interferometer RadioAstron allow to achieve ultra-high angular resolutions. The possibility of detection of a maser emission with resolutions about tens of micro-arcseconds was arguable before successful experiments reported in this paper. We present the results of the maser survey obtained by RadioAstron during first 5 years of operation. Extremely high angular resolution of 11 microarcseconds have been achieved in observations of the megamaser galaxy NGC 4258. For the galaxy at the distance about 7 Mpc this corresponds to linear resolution around 80 AU. Very compact features with angular sizes about 20 micro-arcseconds have been detected in star-forming regions of our Galaxy. Corresponding linear sizes are about 5-10 millions of kilometers.

High-resolution compact spectrometer based on a custom-printed varied-line-spacing concave blazed grating.

PubMed

Chen, Jianwei; Chen, Wang; Zhang, Guodong; Lin, Hui; Chen, Shih-Chi

2017-05-29

We present the modeling, design and characterization of a compact spectrometer, achieving a resolution better than 1.5 nm throughout the visible spectrum (360-825 nm). The key component in the spectrometer is a custom-printed varied-line-space (VLS) concave blazed grating, where the groove density linearly decreases from the center of the grating (530 g/mm) at a rate of 0.58 nm/mm to the edge (528 g/mm). Parametric models have been established to deterministically link the system performance with the VLS grating design parameters, e.g., groove density, line-space varying rate, and to minimize the system footprint. Simulations have been performed in ZEMAX to confirm the results, indicating a 15% enhancement in system resolution versus common constant line-space (CLS) gratings. Next, the VLS concave blazed grating is fabricated via our vacuum nanoimprinting system, where a polydimethylsiloxane (PDMS) stamp is non-uniformly expanded to form the varied-line-spacing pattern from a planar commercial grating master (600 g/mm) for precision imprinting. The concave blazed grating is measured to have an absolute diffraction efficiency of 43%, higher than typical holographic gratings (~30%) used in the commercial compact spectrometers. The completed compact spectrometer contains only one optical component, i.e., the VLS concave grating, as well as an entrance slit and linear photodetector array, achieving a footprint of 11 × 11 × 3 cm 3 , which makes it the most compact and resolving (1.46 nm) spectrometer of its kind.

All-optical optoacoustic microscopy system based on probe beam deflection technique

NASA Astrophysics Data System (ADS)

Maswadi, Saher M.; Tsyboulskic, Dmitri; Roth, Caleb C.; Glickman, Randolph D.; Beier, Hope T.; Oraevsky, Alexander A.; Ibey, Bennett L.

2016-03-01

It is difficult to achieve sub-micron resolution in backward mode OA microscopy using conventional piezoelectric detectors, because of wavefront distortions caused by components placed in the optical path, between the sample and the objective lens, that are required to separate the acoustic wave from the optical beam. As an alternate approach, an optoacoustic microscope (OAM) was constructed using the probe beam deflection technique (PBDT) to detect laserinduced acoustic signals. The all-optical OAM detects laser-generated pressure waves using a probe beam passing through a coupling medium, such as water, filling the space between the microscope objective lens and sample. The acoustic waves generated in the sample propagate through the coupling medium, causing transient changes in the refractive index that deflect the probe beam. These deflections are measured with a high-speed, balanced photodiode position detector. The deflection amplitude is directly proportional to the magnitude of the acoustic pressure wave, and provides the data required for image reconstruction. The sensitivity of the PBDT detector expressed as noise equivalent pressure was 12 Pa, comparable to that of existing high-performance ultrasound detectors. Because of the unimpeded working distance, a high numerical aperture objective lens, i.e. NA = 1, was employed in the OAM to achieve near diffraction-limited lateral resolution of 0.5 μm at 532nm. The all-optical OAM provides several benefits over current piezoelectric detector-based systems, such as increased lateral and axial resolution, higher sensitivity, robustness, and potentially more compatibility with multimodal instruments.

Generation of a pseudo-2D shear-wave velocity section by inversion of a series of 1D dispersion curves

USGS Publications Warehouse

Luo, Y.; Xia, J.; Liu, J.; Xu, Y.; Liu, Q.

2008-01-01

Multichannel Analysis of Surface Waves utilizes a multichannel recording system to estimate near-surface shear (S)-wave velocities from high-frequency Rayleigh waves. A pseudo-2D S-wave velocity (vS) section is constructed by aligning 1D models at the midpoint of each receiver spread and using a spatial interpolation scheme. The horizontal resolution of the section is therefore most influenced by the receiver spread length and the source interval. The receiver spread length sets the theoretical lower limit and any vS structure with its lateral dimension smaller than this length will not be properly resolved in the final vS section. A source interval smaller than the spread length will not improve the horizontal resolution because spatial smearing has already been introduced by the receiver spread. In this paper, we first analyze the horizontal resolution of a pair of synthetic traces. Resolution analysis shows that (1) a pair of traces with a smaller receiver spacing achieves higher horizontal resolution of inverted S-wave velocities but results in a larger relative error; (2) the relative error of the phase velocity at a high frequency is smaller than at a low frequency; and (3) a relative error of the inverted S-wave velocity is affected by the signal-to-noise ratio of data. These results provide us with a guideline to balance the trade-off between receiver spacing (horizontal resolution) and accuracy of the inverted S-wave velocity. We then present a scheme to generate a pseudo-2D S-wave velocity section with high horizontal resolution using multichannel records by inverting high-frequency surface-wave dispersion curves calculated through cross-correlation combined with a phase-shift scanning method. This method chooses only a pair of consecutive traces within a shot gather to calculate a dispersion curve. We finally invert surface-wave dispersion curves of synthetic and real-world data. Inversion results of both synthetic and real-world data demonstrate that inverting high-frequency surface-wave dispersion curves - by a pair of traces through cross-correlation with phase-shift scanning method and with the damped least-square method and the singular-value decomposition technique - can feasibly achieve a reliable pseudo-2D S-wave velocity section with relatively high horizontal resolution. ?? 2008 Elsevier B.V. All rights reserved.

Recent advances in time series InSAR

NASA Astrophysics Data System (ADS)

Hooper, Andrew; Bekaert, David; Spaans, Karsten

2010-05-01

Despite the multiple successes of InSAR at measuring surface displacement, in many instances the signal over much of an image either decorrelates too quickly to be useful or is swamped by atmospheric noise. Time series InSAR methods seek to address these issues by essentially increasing the signal-to-noise ratio (SNR) through the use of more data. These techniques are particularly useful for applications where the strain rates detected at the surface are low, such as postseismic/interseismic motion, magma/fluid movement, landslides and reservoir exploitation. Our previous developments in this field have included a persistent scatterer algorithm based on spatial correlation, a full resolution small baseline approach based on the same strategy, and procedure for combining the two [Hooper, GRL, 2008]. This combined method works well on small areas (up to one frame) at ERS or Envisat strip-map resolution. However, in applying it to larger areas, such as the Guerrero region of Mexico and western Anatolia in Turkey, or when processing data at higher resolution, e.g. from TerraSAR-X, computer resource problems can arise. We have therefore altered the processing strategy to involve smarter use of computer memory. Further improvement is achieved by the resampling of the selected pixels (whether persistent scatterers or distributed scatterers) to a coarser resolution - usually we do not require a resolution on the scale of individual resolution cells for geophysical applications. Aliasing is avoided by summing the phase of nearby selected pixels, weighted according to their estimated SNR. This is akin to smart multilooking, but note that better results can be achieved than by starting the analysis with low-resolution (multilooked) data. Another development concerns selecting pixels only in images where they appear reliable. This allows for resolution cells that become correlated/decorrelated either in a temporary fashion, e.g., due to snow cover, or in a permanent way due to the appearance or removal of scatterers. The detection algorithm relies on the degree of spatial correlation for the pixel of interest in each image. We have also modified our 3-D phase-unwrapping algorithms to allow for the resulting differing combinations of coherent pixels in every interferogram. We demonstrate our improved techniques on volcanoes in Iceland and the 2006 slow-slip event in Guerrero, Mexico.

Extended-range high-resolution dynamical downscaling over a continental-scale spatial domain with atmospheric and surface nudging

NASA Astrophysics Data System (ADS)

Husain, S. Z.; Separovic, L.; Yu, W.; Fernig, D.

2014-12-01

Extended-range high-resolution mesoscale simulations with limited-area atmospheric models when applied to downscale regional analysis fields over large spatial domains can provide valuable information for many applications including the weather-dependent renewable energy industry. Long-term simulations over a continental-scale spatial domain, however, require mechanisms to control the large-scale deviations in the high-resolution simulated fields from the coarse-resolution driving fields. As enforcement of the lateral boundary conditions is insufficient to restrict such deviations, large scales in the simulated high-resolution meteorological fields are therefore spectrally nudged toward the driving fields. Different spectral nudging approaches, including the appropriate nudging length scales as well as the vertical profiles and temporal relaxations for nudging, have been investigated to propose an optimal nudging strategy. Impacts of time-varying nudging and generation of hourly analysis estimates are explored to circumvent problems arising from the coarse temporal resolution of the regional analysis fields. Although controlling the evolution of the atmospheric large scales generally improves the outputs of high-resolution mesoscale simulations within the surface layer, the prognostically evolving surface fields can nevertheless deviate from their expected values leading to significant inaccuracies in the predicted surface layer meteorology. A forcing strategy based on grid nudging of the different surface fields, including surface temperature, soil moisture, and snow conditions, toward their expected values obtained from a high-resolution offline surface scheme is therefore proposed to limit any considerable deviation. Finally, wind speed and temperature at wind turbine hub height predicted by different spectrally nudged extended-range simulations are compared against observations to demonstrate possible improvements achievable using higher spatiotemporal resolution.

Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations

PubMed Central

Gustafsson, Nils; Culley, Siân; Ashdown, George; Owen, Dylan M.; Pereira, Pedro Matos; Henriques, Ricardo

2016-01-01

Despite significant progress, high-speed live-cell super-resolution studies remain limited to specialized optical setups, generally requiring intense phototoxic illumination. Here, we describe a new analytical approach, super-resolution radial fluctuations (SRRF), provided as a fast graphics processing unit-enabled ImageJ plugin. In the most challenging data sets for super-resolution, such as those obtained in low-illumination live-cell imaging with GFP, we show that SRRF is generally capable of achieving resolutions better than 150 nm. Meanwhile, for data sets similar to those obtained in PALM or STORM imaging, SRRF achieves resolutions approaching those of standard single-molecule localization analysis. The broad applicability of SRRF and its performance at low signal-to-noise ratios allows super-resolution using modern widefield, confocal or TIRF microscopes with illumination orders of magnitude lower than methods such as PALM, STORM or STED. We demonstrate this by super-resolution live-cell imaging over timescales ranging from minutes to hours. PMID:27514992

Imaging X-ray spectrophotometers

NASA Technical Reports Server (NTRS)

Hailey, C. J.; Hamilton, T. T.; Ku, W. H.-M.

1981-01-01

A new instrument which combines the good energy resolution of the gas scintillation proportional counter with the high position resolution of the microchannel plate is proposed. A study of the factors which determine the combined energy and position resolution of the new instrument is discussed. Submillimeter position resolution along with good energy resolution (8% fwhm at 6 keV) should be achievable.

MO-G-17A-01: Innovative High-Performance PET Imaging System for Preclinical Imaging and Translational Researches

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sun, X; Lou, K; Rice University, Houston, TX

Purpose: To develop a practical and compact preclinical PET with innovative technologies for substantially improved imaging performance required for the advanced imaging applications. Methods: Several key components of detector, readout electronics and data acquisition have been developed and evaluated for achieving leapfrogged imaging performance over a prototype animal PET we had developed. The new detector module consists of an 8×8 array of 1.5×1.5×30 mm{sup 3} LYSO scintillators with each end coupled to a latest 4×4 array of 3×3 mm{sup 2} Silicon Photomultipliers (with ∼0.2 mm insensitive gap between pixels) through a 2.0 mm thick transparent light spreader. Scintillator surface andmore » reflector/coupling were designed and fabricated to reserve air-gap to achieve higher depth-of-interaction (DOI) resolution and other detector performance. Front-end readout electronics with upgraded 16-ch ASIC was newly developed and tested, so as the compact and high density FPGA based data acquisition and transfer system targeting 10M/s coincidence counting rate with low power consumption. The new detector module performance of energy, timing and DOI resolutions with the data acquisition system were evaluated. Initial Na-22 point source image was acquired with 2 rotating detectors to assess the system imaging capability. Results: No insensitive gaps at the detector edge and thus it is capable for tiling to a large-scale detector panel. All 64 crystals inside the detector were clearly separated from a flood-source image. Measured energy, timing, and DOI resolutions are around 17%, 2.7 ns and 1.96 mm (mean value). Point source image is acquired successfully without detector/electronics calibration and data correction. Conclusion: Newly developed advanced detector and readout electronics will be enable achieving targeted scalable and compact PET system in stationary configuration with >15% sensitivity, ∼1.3 mm uniform imaging resolution, and fast acquisition counting rate capability for substantially improved imaging and quantification performance for small animal imaging and image-guided radiotherapy applications. This work was supported by a research award RP120326 from Cancer Prevention and Research Institute of Texas.« less

Preparation of the CARMENES Input Catalogue: Low- and High-resolution Spectroscopy of M dwarfs

NASA Astrophysics Data System (ADS)

Alonso-Floriano, F. J.; Montes, D.; Caballero, J. A.; Klutsch, A.; Jeffers, S.; Reiners, A.; Zechmeister, M.; Lamert, A.; Passegger, V. M.; Mundt, R.; Amado, P. J.; Berdinas, Z. M.; Casal, E.; Cortés-Contreras, M.; Morales, J. C.; Ribas, I.; Rodríguez-López, C.; Quirrenbach, A.

2015-01-01

The identification of the most promising targets for exoplanet hunting is a crucial first step to ensure an efficient use of the CARMENES guaranteed time. To achieve this, we obtained low-resolution (R ˜ 1500) spectra of 752 M (and late K) dwarfs mostly fainter than J = 9 mag with CAFOS. For all of them, we derived spectral types with 0.5 subtypes accuracy. We also studied metallicity and surface gravity through spectral indices, and activity from pEW(Hα). Next, we observed over 600 M dwarfs at higher resolution (R = 30 000-48 000) with FEROS, CAFE and HRS. We determined rotational velocities, v sin{i} (±0.2-0.5 km s^{-1}), and radial velocities, V_r (±0.1-0.2 km s^{-1}), of the observed stars. From our observations, we identified high-activity, low-metallicity and low-gravity stars, single- and double-lined spectroscopic binaries and, specially, fast rotators, which should be discarded from any target list for exoplanet searches. Here we present preliminary results.

Direct fusion of geostationary meteorological satellite visible and infrared images based on thermal physical properties.

PubMed

Han, Lei; Wulie, Buzha; Yang, Yiling; Wang, Hongqing

2015-01-05

This study investigated a novel method of fusing visible (VIS) and infrared (IR) images with the major objective of obtaining higher-resolution IR images. Most existing image fusion methods focus only on visual performance and many fail to consider the thermal physical properties of the IR images, leading to spectral distortion in the fused image. In this study, we use the IR thermal physical property to correct the VIS image directly. Specifically, the Stefan-Boltzmann Law is used as a strong constraint to modulate the VIS image, such that the fused result shows a similar level of regional thermal energy as the original IR image, while preserving the high-resolution structural features from the VIS image. This method is an improvement over our previous study, which required VIS-IR multi-wavelet fusion before the same correction method was applied. The results of experiments show that applying this correction to the VIS image directly without multi-resolution analysis (MRA) processing achieves similar results, but is considerably more computationally efficient, thereby providing a new perspective on VIS and IR image fusion.

Direct Fusion of Geostationary Meteorological Satellite Visible and Infrared Images Based on Thermal Physical Properties

PubMed Central

Han, Lei; Wulie, Buzha; Yang, Yiling; Wang, Hongqing

2015-01-01

This study investigated a novel method of fusing visible (VIS) and infrared (IR) images with the major objective of obtaining higher-resolution IR images. Most existing image fusion methods focus only on visual performance and many fail to consider the thermal physical properties of the IR images, leading to spectral distortion in the fused image. In this study, we use the IR thermal physical property to correct the VIS image directly. Specifically, the Stefan-Boltzmann Law is used as a strong constraint to modulate the VIS image, such that the fused result shows a similar level of regional thermal energy as the original IR image, while preserving the high-resolution structural features from the VIS image. This method is an improvement over our previous study, which required VIS-IR multi-wavelet fusion before the same correction method was applied. The results of experiments show that applying this correction to the VIS image directly without multi-resolution analysis (MRA) processing achieves similar results, but is considerably more computationally efficient, thereby providing a new perspective on VIS and IR image fusion. PMID:25569749

A spectral-knowledge-based approach for urban land-cover discrimination

NASA Technical Reports Server (NTRS)

Wharton, Stephen W.

1987-01-01

A prototype expert system was developed to demonstrate the feasibility of classifying multispectral remotely sensed data on the basis of spectral knowledge. The spectral expert was developed and tested with Thematic Mapper Simulator (TMS) data having eight spectral bands and a spatial resolution of 5 m. A knowledge base was developed that describes the target categories in terms of characteristic spectral relationships. The knowledge base was developed under the following assumptions: the data are calibrated to ground reflectance, the area is well illuminated, the pixels are dominated by a single category, and the target categories can be recognized without the use of spatial knowledge. Classification decisions are made on the basis of convergent evidence as derived from applying the spectral rules to a multiple spatial resolution representation of the image. The spectral expert achieved an accuracy of 80-percent correct or higher in recognizing 11 spectral categories in TMS data for the washington, DC, area. Classification performance can be expected to decrease for data that do not satisfy the above assumptions as illustrated by the 63-percent accuracy for 30-m resolution Thematic Mapper data.

Imaging nanoscale spatial modulation of a relativistic electron beam with a MeV ultrafast electron microscope

NASA Astrophysics Data System (ADS)

Lu, Chao; Jiang, Tao; Liu, Shengguang; Wang, Rui; Zhao, Lingrong; Zhu, Pengfei; Liu, Yaqi; Xu, Jun; Yu, Dapeng; Wan, Weishi; Zhu, Yimei; Xiang, Dao; Zhang, Jie

2018-03-01

An accelerator-based MeV ultrafast electron microscope (MUEM) has been proposed as a promising tool to the study structural dynamics at the nanometer spatial scale and the picosecond temporal scale. Here, we report experimental tests of a prototype MUEM where high quality images with nanoscale fine structures were recorded with a pulsed ˜3 MeV picosecond electron beam. The temporal and spatial resolutions of the MUEM operating in the single-shot mode are about 4 ps (FWHM) and 100 nm (FWHM), corresponding to a temporal-spatial resolution of 4 × 10-19 s m, about 2 orders of magnitude higher than that achieved with state-of-the-art single-shot keV UEM. Using this instrument, we offer the demonstration of visualizing the nanoscale periodic spatial modulation of an electron beam, which may be converted into longitudinal density modulation through emittance exchange to enable production of high-power coherent radiation at short wavelengths. Our results mark a great step towards single-shot nanometer-resolution MUEMs and compact intense x-ray sources that may have widespread applications in many areas of science.

High-Resolution Microscopy-Coil MR Imaging of Skin Tumors: Techniques and Novel Clinical Applications.

PubMed

Budak, Matthew J; Weir-McCall, Jonathan R; Yeap, Phey M; White, Richard D; Waugh, Shelley A; Sudarshan, Thiru A P; Zealley, Ian A

2015-01-01

High-resolution magnetic resonance (MR) imaging performed with a microscopy coil is a robust radiologic tool for the evaluation of skin lesions. Microscopy-coil MR imaging uses a small surface coil and a 1.5-T or higher MR imaging system. Simple T1- and T2-weighted imaging protocols can be implemented to yield high-quality, high-spatial-resolution images that provide an excellent depiction of dermal anatomy. The primary application of microscopy-coil MR imaging is to delineate the deep margins of skin tumors, thereby providing a preoperative road map for dermatologic surgeons. This information is particularly useful for surgeons who perform Mohs micrographic surgery and in cases of nasofacial neoplasms, where the underlying anatomy is complex. Basal cell carcinoma is the most common nonmelanocytic skin tumor and has a predilection to manifest on the face, where it can be challenging to achieve complete surgical excision while preserving the cosmetic dignity of the patient. Microscopy-coil MR imaging provides dermatologic surgeons with valuable preoperative anatomic information that is not available at conventional clinical examination. ©RSNA, 2015.

Micromachined PIN-PMN-PT crystal composite transducer for high-frequency intravascular ultrasound (IVUS) imaging.

PubMed

Li, Xiang; Ma, Teng; Tian, Jian; Han, Pengdi; Zhou, Qifa; Shung, K Kirk

2014-07-01

In this paper, we report the use of micromachined PbIn1/2Nb1/2O3-PbMg1/3Nb2/3O3-PbTiO 3 (PIN-PMNPT) single crystal 1-3 composite material for intravascular ultrasound (IVUS) imaging application. The effective electromechanical coupling coefficient kt(eff) of the composite was measured to be 0.75 to 0.78. Acoustic impedance was estimated to be 20 MRayl. Based on the composite, needle-type and flexible-type IVUS transducers were fabricated. The composite transducer achieved an 86% bandwidth at the center frequency of 41 MHz, which resulted in a 43 μm axial resolution. Ex vivo IVUS imaging was conducted to demonstrate the improvement of axial resolution. The composite transducer was capable of identifying the three layers of a cadaver coronary artery specimen with high resolution. The PIN-PMN-PT-based composite has superior piezoelectric properties comparable to PMN-PT-based composite and its thermal stability is higher than PMN-PT. PIN-PMN-PT crystal can be an alternative approach for fabricating high-frequency composite, instead of using PMN-PT.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ting Yuan-Sen; Conroy, Charlie; Cargile, Phillip

Understanding the evolution of the Milky Way calls for the precise abundance determination of many elements in many stars. A common perception is that deriving more than a few elemental abundances ([Fe/H], [ α /Fe], perhaps [C/H], [N/H]) requires medium-to-high spectral resolution, R ≳ 10,000, mostly to overcome the effects of line blending. In a recent work, we presented an efficient and practical way to model the full stellar spectrum, even when fitting a large number of stellar labels simultaneously. In this paper, we quantify to what precision the abundances of many different elements can be recovered, as a functionmore » of spectroscopic resolution and wavelength range. In the limit of perfect spectral models and spectral normalization, we show that the precision of elemental abundances is nearly independent of resolution, for a fixed exposure time and number of detector pixels; low-resolution spectra simply afford much higher S/N per pixel and generally larger wavelength range in a single setting. We also show that estimates of most stellar labels are not strongly correlated with one another once R ≳ 1000. Modest errors in the line-spread function, as well as small radial velocity errors, do not affect these conclusions, and data-driven models indicate that spectral (continuum) normalization can be achieved well enough in practice. These results, to be confirmed with an analysis of observed low-resolution data, open up new possibilities for the design of large spectroscopic stellar surveys and for the reanalysis of archival low-resolution data sets.« less

A multi-emitter fitting algorithm for potential live cell super-resolution imaging over a wide range of molecular densities.

PubMed

Takeshima, T; Takahashi, T; Yamashita, J; Okada, Y; Watanabe, S

2018-05-25

Multi-emitter fitting algorithms have been developed to improve the temporal resolution of single-molecule switching nanoscopy, but the molecular density range they can analyse is narrow and the computation required is intensive, significantly limiting their practical application. Here, we propose a computationally fast method, wedged template matching (WTM), an algorithm that uses a template matching technique to localise molecules at any overlapping molecular density from sparse to ultrahigh density with subdiffraction resolution. WTM achieves the localization of overlapping molecules at densities up to 600 molecules μm -2 with a high detection sensitivity and fast computational speed. WTM also shows localization precision comparable with that of DAOSTORM (an algorithm for high-density super-resolution microscopy), at densities up to 20 molecules μm -2 , and better than DAOSTORM at higher molecular densities. The application of WTM to a high-density biological sample image demonstrated that it resolved protein dynamics from live cell images with subdiffraction resolution and a temporal resolution of several hundred milliseconds or less through a significant reduction in the number of camera images required for a high-density reconstruction. WTM algorithm is a computationally fast, multi-emitter fitting algorithm that can analyse over a wide range of molecular densities. The algorithm is available through the website. https://doi.org/10.17632/bf3z6xpn5j.1. © 2018 The Authors. Journal of Microscopy published by JohnWiley & Sons Ltd on behalf of Royal Microscopical Society.

A novel fast gas chromatography method for higher time resolution measurements of speciated monoterpenes in air

NASA Astrophysics Data System (ADS)

Jones, C. E.; Kato, S.; Nakashima, Y.; Kajii, Y.

2014-05-01

Biogenic emissions supply the largest fraction of non-methane volatile organic compounds (VOC) from the biosphere to the atmospheric boundary layer, and typically comprise a complex mixture of reactive terpenes. Due to this chemical complexity, achieving comprehensive measurements of biogenic VOC (BVOC) in air within a satisfactory time resolution is analytically challenging. To address this, we have developed a novel, fully automated Fast Gas Chromatography (Fast-GC) based technique to provide higher time resolution monitoring of monoterpenes (and selected other C9-C15 terpenes) during plant emission studies and in ambient air. To our knowledge, this is the first study to apply a Fast-GC based separation technique to achieve quantification of terpenes in ambient air. Three chromatography methods have been developed for atmospheric terpene analysis under different sampling scenarios. Each method facilitates chromatographic separation of selected BVOC within a significantly reduced analysis time compared to conventional GC methods, whilst maintaining the ability to quantify individual monoterpene structural isomers. Using this approach, the C9-C15 BVOC composition of single plant emissions may be characterised within a 14.5 min analysis time. Moreover, in-situ quantification of 12 monoterpenes in unpolluted ambient air may be achieved within an 11.7 min chromatographic separation time (increasing to 19.7 min when simultaneous quantification of multiple oxygenated C9-C10 terpenoids is required, and/or when concentrations of anthropogenic VOC are significant). These analysis times potentially allow for a twofold to fivefold increase in measurement frequency compared to conventional GC methods. Here we outline the technical details and analytical capability of this chromatographic approach, and present the first in-situ Fast-GC observations of 6 monoterpenes and the oxygenated BVOC (OBVOC) linalool in ambient air. During this field deployment within a suburban forest ~30 km west of central Tokyo, Japan, the Fast-GC limit of detection with respect to monoterpenes was 4-5 ppt, and the agreement between Fast-GC and PTR-MS derived total monoterpene mixing ratios was consistent with previous GC/PTR-MS comparisons. The measurement uncertainties associated with the Fast-GC quantification of monoterpenes are ≤ 12%, while larger uncertainties (up to ~25%) are associated with the OBVOC and sesquiterpene measurements.

Playing fair: the contribution of high-functioning recess to overall school climate in low-income elementary schools.

PubMed

London, Rebecca A; Westrich, Lisa; Stokes-Guinan, Katie; McLaughlin, Milbrey

2015-01-01

Recess is a part of the elementary school day with strong implications for school climate. Positive school climate has been linked to a host of favorable student outcomes, from attendance to achievement. We examine 6 low-income elementary schools' experiences implementing a recess-based program designed to provide safe, healthy, and inclusive play to study how improving recess functioning can affect school climate. Data from teacher, principal, and recess coach interviews; student focus groups; recess observations; and a teacher survey are triangulated to understand the ways that recess changed during implementation. Comparing schools that achieved higher- and lower-functioning recesses, we link recess functioning with school climate. Recess improved in all schools, but 4 of the 6 achieved a higher-functioning recess. In these schools, teachers and principals agreed that by the end of the year, recess offered opportunities for student engagement, conflict resolution, pro-social skill development, and emotional and physical safety. Respondents in these four schools linked these changes to improved overall school climate. Recess is an important part of the school day for contributing to school climate. Creating a positive recess climate helps students to be engaged in meaningful play and return to class ready to learn. © 2014, American School Health Association.

A Comparison of Spatial and Spectral Image Resolution for Mapping Invasive Plants in Coastal California

NASA Astrophysics Data System (ADS)

Underwood, Emma C.; Ustin, Susan L.; Ramirez, Carlos M.

2007-01-01

We explored the potential of detecting three target invasive species: iceplant ( Carpobrotus edulis), jubata grass ( Cortaderia jubata), and blue gum ( Eucalyptus globulus) at Vandenberg Air Force Base, California. We compared the accuracy of mapping six communities (intact coastal scrub, iceplant invaded coastal scrub, iceplant invaded chaparral, jubata grass invaded chaparral, blue gum invaded chaparral, and intact chaparral) using four images with different combinations of spatial and spectral resolution: hyperspectral AVIRIS imagery (174 wavebands, 4 m spatial resolution), spatially degraded AVIRIS (174 bands, 30 m), spectrally degraded AVIRIS (6 bands, 4 m), and both spatially and spectrally degraded AVIRIS (6 bands, 30 m, i.e., simulated Landsat ETM data). Overall success rates for classifying the six classes was 75% (kappa 0.7) using full resolution AVIRIS, 58% (kappa 0.5) for the spatially degraded AVIRIS, 42% (kappa 0.3) for the spectrally degraded AVIRIS, and 37% (kappa 0.3) for the spatially and spectrally degraded AVIRIS. A true Landsat ETM image was also classified to illustrate that the results from the simulated ETM data were representative, which provided an accuracy of 50% (kappa 0.4). Mapping accuracies using different resolution images are evaluated in the context of community heterogeneity (species richness, diversity, and percent species cover). Findings illustrate that higher mapping accuracies are achieved with images possessing high spectral resolution, thus capturing information across the visible and reflected infrared solar spectrum. Understanding the tradeoffs in spectral and spatial resolution can assist land managers in deciding the most appropriate imagery with respect to target invasives and community characteristics.

Multiscale Exploration of Mouse Brain Microstructures Using the Knife-Edge Scanning Microscope Brain Atlas

PubMed Central

Chung, Ji Ryang; Sung, Chul; Mayerich, David; Kwon, Jaerock; Miller, Daniel E.; Huffman, Todd; Keyser, John; Abbott, Louise C.; Choe, Yoonsuck

2011-01-01

Connectomics is the study of the full connection matrix of the brain. Recent advances in high-throughput, high-resolution 3D microscopy methods have enabled the imaging of whole small animal brains at a sub-micrometer resolution, potentially opening the road to full-blown connectomics research. One of the first such instruments to achieve whole-brain-scale imaging at sub-micrometer resolution is the Knife-Edge Scanning Microscope (KESM). KESM whole-brain data sets now include Golgi (neuronal circuits), Nissl (soma distribution), and India ink (vascular networks). KESM data can contribute greatly to connectomics research, since they fill the gap between lower resolution, large volume imaging methods (such as diffusion MRI) and higher resolution, small volume methods (e.g., serial sectioning electron microscopy). Furthermore, KESM data are by their nature multiscale, ranging from the subcellular to the whole organ scale. Due to this, visualization alone is a huge challenge, before we even start worrying about quantitative connectivity analysis. To solve this issue, we developed a web-based neuroinformatics framework for efficient visualization and analysis of the multiscale KESM data sets. In this paper, we will first provide an overview of KESM, then discuss in detail the KESM data sets and the web-based neuroinformatics framework, which is called the KESM brain atlas (KESMBA). Finally, we will discuss the relevance of the KESMBA to connectomics research, and identify challenges and future directions. PMID:22275895

Mapping under-5 and neonatal mortality in Africa, 2000-15: a baseline analysis for the Sustainable Development Goals.

PubMed

Golding, Nick; Burstein, Roy; Longbottom, Joshua; Browne, Annie J; Fullman, Nancy; Osgood-Zimmerman, Aaron; Earl, Lucas; Bhatt, Samir; Cameron, Ewan; Casey, Daniel C; Dwyer-Lindgren, Laura; Farag, Tamer H; Flaxman, Abraham D; Fraser, Maya S; Gething, Peter W; Gibson, Harry S; Graetz, Nicholas; Krause, L Kendall; Kulikoff, Xie Rachel; Lim, Stephen S; Mappin, Bonnie; Morozoff, Chloe; Reiner, Robert C; Sligar, Amber; Smith, David L; Wang, Haidong; Weiss, Daniel J; Murray, Christopher J L; Moyes, Catherine L; Hay, Simon I

2017-11-11

During the Millennium Development Goal (MDG) era, many countries in Africa achieved marked reductions in under-5 and neonatal mortality. Yet the pace of progress toward these goals substantially varied at the national level, demonstrating an essential need for tracking even more local trends in child mortality. With the adoption of the Sustainable Development Goals (SDGs) in 2015, which established ambitious targets for improving child survival by 2030, optimal intervention planning and targeting will require understanding of trends and rates of progress at a higher spatial resolution. In this study, we aimed to generate high-resolution estimates of under-5 and neonatal all-cause mortality across 46 countries in Africa. We assembled 235 geographically resolved household survey and census data sources on child deaths to produce estimates of under-5 and neonatal mortality at a resolution of 5 × 5 km grid cells across 46 African countries for 2000, 2005, 2010, and 2015. We used a Bayesian geostatistical analytical framework to generate these estimates, and implemented predictive validity tests. In addition to reporting 5 × 5 km estimates, we also aggregated results obtained from these estimates into three different levels-national, and subnational administrative levels 1 and 2-to provide the full range of geospatial resolution that local, national, and global decision makers might require. Amid improving child survival in Africa, there was substantial heterogeneity in absolute levels of under-5 and neonatal mortality in 2015, as well as the annualised rates of decline achieved from 2000 to 2015. Subnational areas in countries such as Botswana, Rwanda, and Ethiopia recorded some of the largest decreases in child mortality rates since 2000, positioning them well to achieve SDG targets by 2030 or earlier. Yet these places were the exception for Africa, since many areas, particularly in central and western Africa, must reduce under-5 mortality rates by at least 8·8% per year, between 2015 and 2030, to achieve the SDG 3.2 target for under-5 mortality by 2030. In the absence of unprecedented political commitment, financial support, and medical advances, the viability of SDG 3.2 achievement in Africa is precarious at best. By producing under-5 and neonatal mortality rates at multiple levels of geospatial resolution over time, this study provides key information for decision makers to target interventions at populations in the greatest need. In an era when precision public health increasingly has the potential to transform the design, implementation, and impact of health programmes, our 5 × 5 km estimates of child mortality in Africa provide a baseline against which local, national, and global stakeholders can map the pathways for ending preventable child deaths by 2030. Bill & Melinda Gates Foundation. Copyright © 2017 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license. Published by Elsevier Ltd.. All rights reserved.

Micro-/nanoscale multi-field coupling in nonlinear photonic devices

NASA Astrophysics Data System (ADS)

Yang, Qing; Wang, Yubo; Tang, Mingwei; Xu, Pengfei; Xu, Yingke; Liu, Xu

2017-08-01

The coupling of mechanics/electronics/photonics may improve the performance of nanophotonic devices not only in the linear region but also in the nonlinear region. This review letter mainly presents the recent advances on multi-field coupling in nonlinear photonic devices. The nonlinear piezoelectric effect and piezo-phototronic effects in quantum wells and fibers show that large second-order nonlinear susceptibilities can be achieved, and second harmonic generation and electro-optic modulation can be enhanced and modulated. Strain engineering can tune the lattice structures and induce second order susceptibilities in central symmetry semiconductors. By combining the absorption-based photoacoustic effect and intensity-dependent photobleaching effect, subdiffraction imaging can be achieved. This review will also discuss possible future applications of these novel effects and the perspective of their research. The review can help us develop a deeper knowledge of the substance of photon-electron-phonon interaction in a micro-/nano- system. Moreover, it can benefit the design of nonlinear optical sensors and imaging devices with a faster response rate, higher efficiency, more sensitivity and higher spatial resolution which could be applied in environmental detection, bio-sensors, medical imaging and so on.

Use of noncrystallographic symmetry for automated model building at medium to low resolution.

PubMed

Wiegels, Tim; Lamzin, Victor S

2012-04-01

A novel method is presented for the automatic detection of noncrystallographic symmetry (NCS) in macromolecular crystal structure determination which does not require the derivation of molecular masks or the segmentation of density. It was found that throughout structure determination the NCS-related parts may be differently pronounced in the electron density. This often results in the modelling of molecular fragments of variable length and accuracy, especially during automated model-building procedures. These fragments were used to identify NCS relations in order to aid automated model building and refinement. In a number of test cases higher completeness and greater accuracy of the obtained structures were achieved, specifically at a crystallographic resolution of 2.3 Å or poorer. In the best case, the method allowed the building of up to 15% more residues automatically and a tripling of the average length of the built fragments.

New Physical Algorithms for Downscaling SMAP Soil Moisture

NASA Astrophysics Data System (ADS)

Sadeghi, M.; Ghafari, E.; Babaeian, E.; Davary, K.; Farid, A.; Jones, S. B.; Tuller, M.

2017-12-01

The NASA Soil Moisture Active Passive (SMAP) mission provides new means for estimation of surface soil moisture at the global scale. However, for many hydrological and agricultural applications the spatial SMAP resolution is too low. To address this scale issue we fused SMAP data with MODIS observations to generate soil moisture maps at 1-km spatial resolution. In course of this study we have improved several existing empirical algorithms and introduced a new physical approach for downscaling SMAP data. The universal triangle/trapezoid model was applied to relate soil moisture to optical/thermal observations such as NDVI, land surface temperature and surface reflectance. These algorithms were evaluated with in situ data measured at 5-cm depth. Our results demonstrate that downscaling SMAP soil moisture data based on physical indicators of soil moisture derived from the MODIS satellite leads to higher accuracy than that achievable with empirical downscaling algorithms. Keywords: Soil moisture, microwave data, downscaling, MODIS, triangle/trapezoid model.

New Frontiers and Challenges for Single-Cell Electrochemical Analysis.

PubMed

Zhang, Jingjing; Zhou, Junyu; Pan, Rongrong; Jiang, Dechen; Burgess, James D; Chen, Hong-Yuan

2018-02-23

Previous measurements of cell populations might obscure many important cellular differences, and new strategies for single-cell analyses are urgently needed to re-examine these fundamental biological principles for better diagnosis and treatment of diseases. Electrochemistry is a robust technique for the analysis of single living cells that has the advantages of minor interruption of cellular activity and provides the capability of high spatiotemporal resolution. The achievements of the past 30 years have revealed significant information about the exocytotic events of single cells to elucidate the mechanisms of cellular activity. Currently, the rapid developments of micro/nanofabrication and optoelectronic technologies drive the development of multifunctional electrodes and novel electrochemical approaches with higher resolution for single cells. In this Perspective, three new frontiers in this field, namely, electrochemical microscopy, intracellular analysis, and single-cell analysis in a biological system (i.e., neocortex and retina), are reviewed. The unique features and remaining challenges of these techniques are discussed.

Performance Evaluation and Analysis for Gravity Matching Aided Navigation.

PubMed

Wu, Lin; Wang, Hubiao; Chai, Hua; Zhang, Lu; Hsu, Houtse; Wang, Yong

2017-04-05

Simulation tests were accomplished in this paper to evaluate the performance of gravity matching aided navigation (GMAN). Four essential factors were focused in this study to quantitatively evaluate the performance: gravity database (DB) resolution, fitting degree of gravity measurements, number of samples in matching, and gravity changes in the matching area. Marine gravity anomaly DB derived from satellite altimetry was employed. Actual dynamic gravimetry accuracy and operating conditions were referenced to design the simulation parameters. The results verified that the improvement of DB resolution, gravimetry accuracy, number of measurement samples, or gravity changes in the matching area generally led to higher positioning accuracies, while the effects of them were different and interrelated. Moreover, three typical positioning accuracy targets of GMAN were proposed, and the conditions to achieve these targets were concluded based on the analysis of several different system requirements. Finally, various approaches were provided to improve the positioning accuracy of GMAN.

Performance Evaluation and Analysis for Gravity Matching Aided Navigation

PubMed Central

Wu, Lin; Wang, Hubiao; Chai, Hua; Zhang, Lu; Hsu, Houtse; Wang, Yong

2017-01-01

Simulation tests were accomplished in this paper to evaluate the performance of gravity matching aided navigation (GMAN). Four essential factors were focused in this study to quantitatively evaluate the performance: gravity database (DB) resolution, fitting degree of gravity measurements, number of samples in matching, and gravity changes in the matching area. Marine gravity anomaly DB derived from satellite altimetry was employed. Actual dynamic gravimetry accuracy and operating conditions were referenced to design the simulation parameters. The results verified that the improvement of DB resolution, gravimetry accuracy, number of measurement samples, or gravity changes in the matching area generally led to higher positioning accuracies, while the effects of them were different and interrelated. Moreover, three typical positioning accuracy targets of GMAN were proposed, and the conditions to achieve these targets were concluded based on the analysis of several different system requirements. Finally, various approaches were provided to improve the positioning accuracy of GMAN. PMID:28379178

The structure of plant photosystem I super-complex at 2.8 Å resolution

PubMed Central

Mazor, Yuval; Borovikova, Anna; Nelson, Nathan

2015-01-01

Most life forms on Earth are supported by solar energy harnessed by oxygenic photosynthesis. In eukaryotes, photosynthesis is achieved by large membrane-embedded super-complexes, containing reaction centers and connected antennae. Here, we report the structure of the higher plant PSI-LHCI super-complex determined at 2.8 Å resolution. The structure includes 16 subunits and more than 200 prosthetic groups, which are mostly light harvesting pigments. The complete structures of the four LhcA subunits of LHCI include 52 chlorophyll a and 9 chlorophyll b molecules, as well as 10 carotenoids and 4 lipids. The structure of PSI-LHCI includes detailed protein pigments and pigment–pigment interactions, essential for the mechanism of excitation energy transfer and its modulation in one of nature's most efficient photochemical machines. DOI: http://dx.doi.org/10.7554/eLife.07433.001 PMID:26076232

Reduced-Scale Transition-Edge Sensor Detectors for Solar and X-Ray Astrophysics

NASA Technical Reports Server (NTRS)

Datesman, Aaron M.; Adams, Joseph S.; Bandler, Simon R.; Betancourt-Martinez, Gabriele L.; Chang, Meng-Ping; Chervenak, James A.; Eckart, Megan E.; Ewin, Audrey E.; Finkbeiner, Fred M.; Ha, Jong Yoon;

High-resolution Crystal Structure of Dimeric VP40 From Sudan ebolavirus.

PubMed

Clifton, Matthew C; Bruhn, Jessica F; Atkins, Kateri; Webb, Terry L; Baydo, Ruth O; Raymond, Amy; Lorimer, Donald D; Edwards, Thomas E; Myler, Peter J; Saphire, Erica Ollmann

2015-10-01

Ebolaviruses cause severe hemorrhagic fever. Central to the Ebola life cycle is the matrix protein VP40, which oligomerizes and drives viral budding. Here we present the crystal structure of the Sudan virus (SUDV) matrix protein. This structure is higher resolution (1.6 Å) than previously achievable. Despite differences in the protein purification, we find that it still forms a stable dimer in solution, as was noted for other Ebola VP40s. Although the N-terminal domain interface by which VP40 dimerizes is conserved between Ebola virus and SUDV, the C-terminal domain interface by which VP40 dimers may further assemble is significantly smaller in this SUDV assembly. © The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Subwavelength optical lithography via classical light: A possible implementation

NASA Astrophysics Data System (ADS)

You, Jieyu; Liao, Zeyang; Hemmer, P. R.; Zubairy, M. Suhail

2018-04-01

The resolution of an interferometric optical lithography system is about the half wavelength of the illumination light. We proposed a method based on Doppleron resonance to achieve a resolution beyond half wavelength [Phys. Rev. Lett. 96, 163603 (2006), 10.1103/PhysRevLett.96.163603]. Here, we analyze a possible experimental demonstration of this method in the negatively charged silicon-vacancy (SiV-) system by considering realistic experimental parameters. Our results show that quarter wavelength resolution and beyond can be achieved in this system even in room temperature without using perturbation theory.

High resolution imaging and precision photometric measurements from a small soft-landed lunar telescope --Abstract only

NASA Technical Reports Server (NTRS)

Genet, R. M.; Hine, B.; Drummond, M.; Patterson-Hine, A.; Borucki, W.; Burns, J.; Genet, D.

1994-01-01

The ultimate imaging resolution in the UV and photometric precision achievable with a small (less than 1-meter) telescope located on the Moon is considered. The imaging resolution and photometric precision that might be practically achieved when the effects of the Lunar environment and equipment limitations are accounted for is then suggested. Finally, the practicality of soft landing such a telescope on the moon is considered, along with suggestions of how it might be directly controlled by using astronomers without any significant permanent staff.

Achieving superresolution with illumination-enhanced sparsity.

PubMed

Yu, Jiun-Yann; Becker, Stephen R; Folberth, James; Wallin, Bruce F; Chen, Simeng; Cogswell, Carol J

2018-04-16

Recent advances in superresolution fluorescence microscopy have been limited by a belief that surpassing two-fold resolution enhancement of the Rayleigh resolution limit requires stimulated emission or the fluorophore to undergo state transitions. Here we demonstrate a new superresolution method that requires only image acquisitions with a focused illumination spot and computational post-processing. The proposed method utilizes the focused illumination spot to effectively reduce the object size and enhance the object sparsity and consequently increases the resolution and accuracy through nonlinear image post-processing. This method clearly resolves 70nm resolution test objects emitting ~530nm light with a 1.4 numerical aperture (NA) objective, and, when imaging through a 0.5NA objective, exhibits high spatial frequencies comparable to a 1.4NA widefield image, both demonstrating a resolution enhancement above two-fold of the Rayleigh resolution limit. More importantly, we examine how the resolution increases with photon numbers, and show that the more-than-two-fold enhancement is achievable with realistic photon budgets.

High efficiency multishot interleaved spiral-in/out: acquisition for high-resolution BOLD fMRI.

PubMed

Jung, Youngkyoo; Samsonov, Alexey A; Liu, Thomas T; Buracas, Giedrius T

2013-08-01

Growing demand for high spatial resolution blood oxygenation level dependent (BOLD) functional magnetic resonance imaging faces a challenge of the spatial resolution versus coverage or temporal resolution tradeoff, which can be addressed by methods that afford increased acquisition efficiency. Spiral acquisition trajectories have been shown to be superior to currently prevalent echo-planar imaging in terms of acquisition efficiency, and high spatial resolution can be achieved by employing multiple-shot spiral acquisition. The interleaved spiral in/out trajectory is preferred over spiral-in due to increased BOLD signal contrast-to-noise ratio (CNR) and higher acquisition efficiency than that of spiral-out or noninterleaved spiral in/out trajectories (Law & Glover. Magn Reson Med 2009; 62:829-834.), but to date applicability of the multishot interleaved spiral in/out for high spatial resolution imaging has not been studied. Herein we propose multishot interleaved spiral in/out acquisition and investigate its applicability for high spatial resolution BOLD functional magnetic resonance imaging. Images reconstructed from interleaved spiral-in and -out trajectories possess artifacts caused by differences in T2 decay, off-resonance, and k-space errors associated with the two trajectories. We analyze the associated errors and demonstrate that application of conjugate phase reconstruction and spectral filtering can substantially mitigate these image artifacts. After applying these processing steps, the multishot interleaved spiral in/out pulse sequence yields high BOLD CNR images at in-plane resolution below 1 × 1 mm while preserving acceptable temporal resolution (4 s) and brain coverage (15 slices of 2 mm thickness). Moreover, this method yields sufficient BOLD CNR at 1.5 mm isotropic resolution for detection of activation in hippocampus associated with cognitive tasks (Stern memory task). The multishot interleaved spiral in/out acquisition is a promising technique for high spatial resolution BOLD functional magnetic resonance imaging applications. © 2012 Wiley Periodicals, Inc.

Side readout of long scintillation crystal elements with digital SiPM for TOF-DOI PET.

PubMed

Yeom, Jung Yeol; Vinke, Ruud; Levin, Craig S

2014-12-01

Side readout of scintillation light from crystal elements in positron emission tomography (PET) is an alternative to conventional end-readout configurations, with the benefit of being able to provide accurate depth-of-interaction (DOI) information and good energy resolution while achieving excellent timing resolution required for time-of-flight PET. This paper explores different readout geometries of scintillation crystal elements with the goal of achieving a detector that simultaneously achieves excellent timing resolution, energy resolution, spatial resolution, and photon sensitivity. The performance of discrete LYSO scintillation elements of different lengths read out from the end/side with digital silicon photomultipliers (dSiPMs) has been assessed. Compared to 3 × 3 × 20 mm(3) LYSO crystals read out from their ends with a coincidence resolving time (CRT) of 162 ± 6 ps FWHM and saturated energy spectra, a side-readout configuration achieved an excellent CRT of 144 ± 2 ps FWHM after correcting for timing skews within the dSiPM and an energy resolution of 11.8% ± 0.2% without requiring energy saturation correction. Using a maximum likelihood estimation method on individual dSiPM pixel response that corresponds to different 511 keV photon interaction positions, the DOI resolution of this 3 × 3 × 20 mm(3) crystal side-readout configuration was computed to be 0.8 mm FWHM with negligible artifacts at the crystal ends. On the other hand, with smaller 3 × 3 × 5 mm(3) LYSO crystals that can also be tiled/stacked to provide DOI information, a timing resolution of 134 ± 6 ps was attained but produced highly saturated energy spectra. The energy, timing, and DOI resolution information extracted from the side of long scintillation crystal elements coupled to dSiPM have been acquired for the first time. The authors conclude in this proof of concept study that such detector configuration has the potential to enable outstanding detector performance in terms of timing, energy, and DOI resolution.

Side readout of long scintillation crystal elements with digital SiPM for TOF-DOI PET

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yeom, Jung Yeol, E-mail: yeomjy@kumoh.ac.kr, E-mail: cslevin@stanford.edu; Vinke, Ruud; Levin, Craig S., E-mail: yeomjy@kumoh.ac.kr, E-mail: cslevin@stanford.edu

Purpose: Side readout of scintillation light from crystal elements in positron emission tomography (PET) is an alternative to conventional end-readout configurations, with the benefit of being able to provide accurate depth-of-interaction (DOI) information and good energy resolution while achieving excellent timing resolution required for time-of-flight PET. This paper explores different readout geometries of scintillation crystal elements with the goal of achieving a detector that simultaneously achieves excellent timing resolution, energy resolution, spatial resolution, and photon sensitivity. Methods: The performance of discrete LYSO scintillation elements of different lengths read out from the end/side with digital silicon photomultipliers (dSiPMs) has been assessed.more » Results: Compared to 3 × 3 × 20 mm{sup 3} LYSO crystals read out from their ends with a coincidence resolving time (CRT) of 162 ± 6 ps FWHM and saturated energy spectra, a side-readout configuration achieved an excellent CRT of 144 ± 2 ps FWHM after correcting for timing skews within the dSiPM and an energy resolution of 11.8% ± 0.2% without requiring energy saturation correction. Using a maximum likelihood estimation method on individual dSiPM pixel response that corresponds to different 511 keV photon interaction positions, the DOI resolution of this 3 × 3 × 20 mm{sup 3} crystal side-readout configuration was computed to be 0.8 mm FWHM with negligible artifacts at the crystal ends. On the other hand, with smaller 3 × 3 × 5 mm{sup 3} LYSO crystals that can also be tiled/stacked to provide DOI information, a timing resolution of 134 ± 6 ps was attained but produced highly saturated energy spectra. Conclusions: The energy, timing, and DOI resolution information extracted from the side of long scintillation crystal elements coupled to dSiPM have been acquired for the first time. The authors conclude in this proof of concept study that such detector configuration has the potential to enable outstanding detector performance in terms of timing, energy, and DOI resolution.« less

Feasibility of high-resolution one-dimensional relaxation imaging at low magnetic field using a single-sided NMR scanner applied to articular cartilage

NASA Astrophysics Data System (ADS)

Rössler, Erik; Mattea, Carlos; Stapf, Siegfried

2015-02-01

Low field Nuclear Magnetic Resonance increases the contrast of the longitudinal relaxation rate in many biological tissues; one prominent example is hyaline articular cartilage. In order to take advantage of this increased contrast and to profile the depth-dependent variations, high resolution parameter measurements are carried out which can be of critical importance in an early diagnosis of cartilage diseases such as osteoarthritis. However, the maximum achievable spatial resolution of parameter profiles is limited by factors such as sensor geometry, sample curvature, and diffusion limitation. In this work, we report on high-resolution single-sided NMR scanner measurements with a commercial device, and quantify these limitations. The highest achievable spatial resolution on the used profiler, and the lateral dimension of the sensitive volume were determined. Since articular cartilage samples are usually bent, we also focus on averaging effects inside the horizontally aligned sensitive volume and their impact on the relaxation profiles. Taking these critical parameters into consideration, depth-dependent relaxation time profiles with the maximum achievable vertical resolution of 20 μm are discussed, and are correlated with diffusion coefficient profiles in hyaline articular cartilage in order to reconstruct T2 maps from the diffusion-weighted CPMG decays of apparent relaxation rates.

Do obese children with diabetic ketoacidosis have type 1 or type 2 diabetes?

PubMed

Low, Joey C; Felner, Eric I; Muir, Andrew B; Brown, Milton; Dorcelet, Margalie; Peng, Limin; Umpierrez, Guillermo E

2012-04-01

Many obese children with unprovoked diabetic ketoacidosis (DKA) display clinical features of type 2 diabetes during follow up. We describe the clinical presentation, autoimmune markers and the long-term course of obese and lean children with DKA. We reviewed the medical records on the initial acute hospitalization and outpatient follow-up care of 21 newly diagnosed obese and 20 lean children with unprovoked DKA at Emory University affiliated children's hospitals between 1/2003 and 12/2006. Obese children with DKA were older and predominantly male, had acanthosis nigricans, and had lower prevalence of autoantibodies to islet cells and glutamic acid decarboxylase than lean children. Half of the obese, but none of the lean children with DKA achieve near-normoglycemia remission and discontinued insulin therapy during follow-up. Time to achieve remission was 2.2±2.3 months. There were no differences on clinical presentation between obese children who achieved near-normoglycemia remission versus those who did not. The addition of metformin to insulin therapy shortly after resolution of DKA resulted in lower hemoglobin A1c (HbA1c) levels, higher rates of near-normoglycemia remission, and lower frequency of DKA recurrence. Near-normoglycemia remission, however, was of short duration and the majority of obese patients required reinstitution of insulin treatment within 15 months of follow-up. In contrast to lean children with DKA, many obese children with unprovoked DKA display clinical and immunologic features of type 2 diabetes during follow-up. The addition of metformin to insulin therapy shortly after resolution of DKA improves glycemic control, facilitates achieving near-normoglycemia remission and prevents DKA recurrence in obese children with DKA. Copyright © 2011 Primary Care Diabetes Europe. Published by Elsevier Ltd. All rights reserved.

Ultrathin high-resolution flexographic printing using nanoporous stamps

PubMed Central

Kim, Sanha; Sojoudi, Hossein; Zhao, Hangbo; Mariappan, Dhanushkodi; McKinley, Gareth H.; Gleason, Karen K.; Hart, A. John

2016-01-01

Since its invention in ancient times, relief printing, commonly called flexography, has been used to mass-produce artifacts ranging from decorative graphics to printed media. Now, higher-resolution flexography is essential to manufacturing low-cost, large-area printed electronics. However, because of contact-mediated liquid instabilities and spreading, the resolution of flexographic printing using elastomeric stamps is limited to tens of micrometers. We introduce engineered nanoporous microstructures, comprising polymer-coated aligned carbon nanotubes (CNTs), as a next-generation stamp material. We design and engineer the highly porous microstructures to be wetted by colloidal inks and to transfer a thin layer to a target substrate upon brief contact. We demonstrate printing of diverse micrometer-scale patterns of a variety of functional nanoparticle inks, including Ag, ZnO, WO3, and CdSe/ZnS, onto both rigid and compliant substrates. The printed patterns have highly uniform nanoscale thickness (5 to 50 nm) and match the stamp features with high fidelity (edge roughness, ~0.2 μm). We derive conditions for uniform printing based on nanoscale contact mechanics, characterize printed Ag lines and transparent conductors, and achieve continuous printing at a speed of 0.2 m/s. The latter represents a combination of resolution and throughput that far surpasses industrial printing technologies. PMID:27957542

Re-engineering the stereoscope for the 21st Century

NASA Astrophysics Data System (ADS)

Kollin, Joel S.; Hollander, Ari J.

2007-02-01

While discussing the current state of stereo head-mounted and 3D projection displays, the authors came to the realization that flat-panel LCD displays offer higher resolution than projection for stereo display at a low (and continually dropping) cost. More specifically, where head-mounted displays of moderate resolution and field-of-view cost tens of thousands of dollars, we can achieve an angular resolution approaching that of the human eye with a field-of-view (FOV) greater than 90° for less than $1500. For many immersive applications head tracking is unnecessary and sometimes even undesirable, and a low cost/high quality wide FOV display may significantly increase the application space for 3D display. After outlining the problem and potential of this solution we describe the initial construction of a simple Wheatstone stereoscope using 24" LCD displays and then show engineering improvements that increase the FOV and usability of the system. The applicability of a high-immersion, high-resolution display for art, entertainment, and simulation is presented along with a content production system that utilizes the capabilities of the system. We then discuss the potential use of the system for VR pain control therapy, treatment of post-traumatic stress disorders and other serious games applications.

High-Throughput Effect-Directed Analysis Using Downscaled in Vitro Reporter Gene Assays To Identify Endocrine Disruptors in Surface Water

PubMed Central

2018-01-01

Effect-directed analysis (EDA) is a commonly used approach for effect-based identification of endocrine disruptive chemicals in complex (environmental) mixtures. However, for routine toxicity assessment of, for example, water samples, current EDA approaches are considered time-consuming and laborious. We achieved faster EDA and identification by downscaling of sensitive cell-based hormone reporter gene assays and increasing fractionation resolution to allow testing of smaller fractions with reduced complexity. The high-resolution EDA approach is demonstrated by analysis of four environmental passive sampler extracts. Downscaling of the assays to a 384-well format allowed analysis of 64 fractions in triplicate (or 192 fractions without technical replicates) without affecting sensitivity compared to the standard 96-well format. Through a parallel exposure method, agonistic and antagonistic androgen and estrogen receptor activity could be measured in a single experiment following a single fractionation. From 16 selected candidate compounds, identified through nontargeted analysis, 13 could be confirmed chemically and 10 were found to be biologically active, of which the most potent nonsteroidal estrogens were identified as oxybenzone and piperine. The increased fractionation resolution and the higher throughput that downscaling provides allow for future application in routine high-resolution screening of large numbers of samples in order to accelerate identification of (emerging) endocrine disruptors. PMID:29547277

Low-temperature high-Z gamma-detectors with very high energy resolution

NASA Astrophysics Data System (ADS)

Pobes, Carlos; Brofferio, Chiara; Bucci, Carlo; Cremonesi, Oliviero; Fiorini, Ettore; Giuliani, Andrea; Nucciotti, Angelo; Pavan, Maura; Pedretti, Marisa; Pessina, Gianluigi; Pirro, Stefano; Previtali, Ezio; Sisti, Monica; Vanzini, Marco; Zanotti, Luigi

2001-12-01

High-Z low-temperature calorimeters are developed by an Italian collaboration (Milano-Como-Gran Sasso Underground Laboratories) in order to search for rare nuclear events and Dark Matter massive candidates. They exhibit an excellent energy resolution, close to that of Ge-diodes, but a much higher efficiency. Different high-Z materials were initially employed . A many-years optimisation work on tellurium oxide (TeO2) lead to impressive results: devices with total masses around 750 g present FWHM energy resolutions on gamma-ray peaks ranging from 1 KeV (close to the 5 KeV energy threshold) to 2.6 KeV at 2615 KeV (208Tl gamma line). A 3.2 KeV FWHM energy resolution was obtained at 5.4 MeV (210Po alpha line), which is by far the best one ever achieved with any alpha detector. These devices, operated at about 10 mK, consist of a TeO2 single crystal thermally coupled to a 50 mg Neutron Transmutation Doped (NTD) Ge crystal working as a temperature sensor. Special care was devoted to methods for response linearization and temporal stabilisation. Devices based on the same principle and specifically optimised could find applications in several fields like gamma-ray astrophysics, nuclear physics searches, environmental monitoring and radiation metrology.

High-energy-resolution monochromator for nuclear resonant scattering of synchrotron radiation by Te-125 at 35.49 keV

NASA Astrophysics Data System (ADS)

Imai, Yasuhiko; Yoda, Yoshitaka; Kitao, Shinji; Masuda, Ryo; Higashitaniguchi, Satoshi; Inaba, Chika; Seto, Makoto

2007-09-01

We have developed a high-resolution monochromator (HRM) for the measurement of nuclear resonant scattering (NRS) of synchrotron radiation by Te-125 at 35.49 keV using the backscattering of sapphire (9 1 -10 68). HRMs for nuclei with excitation energies less than 30 keV have been successfully developed using high angle diffractions by silicon crystals. Nearly perfect silicon crystal, however, is not suitable for high efficient HRMs at higher energy regions because the symmetry of the crystal structure is high and the Debye-temperature is low. Therefore, we used high quality synthetic sapphire crystal, which has low symmetry of crystal structure and high Debye-temperature. The temperature of the crystal was precisely controlled around 218 K to diffract synchrotron radiation with a Bragg angle of π/2 - 0.52 mrad. Energy was tuned by changing the crystal temperature under the condition of constant diffraction angle. Energy resolution was measured by detecting nuclear forward scattering by Te-125 in enriched TeO II. The relative energy resolution of 2.1×10 -7 is achieved, that is 7.5 meV in energy bandwidth. This HRM opens studies on element-specific dynamics and electronic state of substances containing Te-125.

Increased biomagnetic activity in the ventral pathway in mild cognitive impairment.

PubMed

Maestú, F; Campo, P; Del Río, D; Moratti, S; Gil-Gregorio, P; Fernández, A; Capilla, A; Ortiz, T

2008-06-01

Mild cognitive impairment (MCI) patients represent an intermediary state between healthy aging and dementia. MCI activation profiles, recorded during a memory task, have been studied either through high spatial resolution or high temporal resolution techniques. However, little is known about the benefit of combining both dimensions. Here, we investigate, by means of magnetoencephalography (MEG), whether spatio-temporal profiles of neuromagnetic activity could differentiate between MCI and age-matched elderly participants. Taking the advantage of the high temporal resolution and good spatial resolution of MEG, neuromagnetic activity from 15 elderly MCI patients and 20 age-matched controls was recorded during the performance of a modified version of the Sternberg paradigm. Behavioral performance was similar in both groups. A between group analysis revealed that MCI patients showed bilateral higher activity in the ventral pathway, in both the target and the non-target stimuli. A within-group analysis of the target stimuli, indicates a lack of asymmetry through all late latency windows in both groups. MCI patients showed a compensatory mechanism represented by an increased bilateral activity of the ventral pathway in order to achieve a behavioral performance similar to the control group. This spatio-temporal pattern of activity could be another tool to differentiate between healthy aging and MCI patients.

Hybrid-denovo: a de novo OTU-picking pipeline integrating single-end and paired-end 16S sequence tags.

PubMed

Chen, Xianfeng; Johnson, Stephen; Jeraldo, Patricio; Wang, Junwen; Chia, Nicholas; Kocher, Jean-Pierre A; Chen, Jun

2018-03-01

Illumina paired-end sequencing has been increasingly popular for 16S rRNA gene-based microbiota profiling. It provides higher phylogenetic resolution than single-end reads due to a longer read length. However, the reverse read (R2) often has significant low base quality, and a large proportion of R2s will be discarded after quality control, resulting in a mixture of paired-end and single-end reads. A typical 16S analysis pipeline usually processes either paired-end or single-end reads but not a mixture. Thus, the quantification accuracy and statistical power will be reduced due to the loss of a large amount of reads. As a result, rare taxa may not be detectable with the paired-end approach, or low taxonomic resolution will result in a single-end approach. To have both the higher phylogenetic resolution provided by paired-end reads and the higher sequence coverage by single-end reads, we propose a novel OTU-picking pipeline, hybrid-denovo, that can process a hybrid of single-end and paired-end reads. Using high-quality paired-end reads as a gold standard, we show that hybrid-denovo achieved the highest correlation with the gold standard and performed better than the approaches based on paired-end or single-end reads in terms of quantifying the microbial diversity and taxonomic abundances. By applying our method to a rheumatoid arthritis (RA) data set, we demonstrated that hybrid-denovo captured more microbial diversity and identified more RA-associated taxa than a paired-end or single-end approach. Hybrid-denovo utilizes both paired-end and single-end 16S sequencing reads and is recommended for 16S rRNA gene targeted paired-end sequencing data.

Digitally controlled analog proportional-integral-derivative (PID) controller for high-speed scanning probe microscopy

NASA Astrophysics Data System (ADS)

Dukic, Maja; Todorov, Vencislav; Andany, Santiago; Nievergelt, Adrian P.; Yang, Chen; Hosseini, Nahid; Fantner, Georg E.

2017-12-01

Nearly all scanning probe microscopes (SPMs) contain a feedback controller, which is used to move the scanner in the direction of the z-axis in order to maintain a constant setpoint based on the tip-sample interaction. The most frequently used feedback controller in SPMs is the proportional-integral (PI) controller. The bandwidth of the PI controller presents one of the speed limiting factors in high-speed SPMs, where higher bandwidths enable faster scanning speeds and higher imaging resolution. Most SPM systems use digital signal processor-based PI feedback controllers, which require analog-to-digital and digital-to-analog converters. These converters introduce additional feedback delays which limit the achievable imaging speed and resolution. In this paper, we present a digitally controlled analog proportional-integral-derivative (PID) controller. The controller implementation allows tunability of the PID gains over a large amplification and frequency range, while also providing precise control of the system and reproducibility of the gain parameters. By using the analog PID controller, we were able to perform successful atomic force microscopy imaging of a standard silicon calibration grating at line rates up to several kHz.

Digitally controlled analog proportional-integral-derivative (PID) controller for high-speed scanning probe microscopy.

PubMed

Dukic, Maja; Todorov, Vencislav; Andany, Santiago; Nievergelt, Adrian P; Yang, Chen; Hosseini, Nahid; Fantner, Georg E

2017-12-01

Nearly all scanning probe microscopes (SPMs) contain a feedback controller, which is used to move the scanner in the direction of the z-axis in order to maintain a constant setpoint based on the tip-sample interaction. The most frequently used feedback controller in SPMs is the proportional-integral (PI) controller. The bandwidth of the PI controller presents one of the speed limiting factors in high-speed SPMs, where higher bandwidths enable faster scanning speeds and higher imaging resolution. Most SPM systems use digital signal processor-based PI feedback controllers, which require analog-to-digital and digital-to-analog converters. These converters introduce additional feedback delays which limit the achievable imaging speed and resolution. In this paper, we present a digitally controlled analog proportional-integral-derivative (PID) controller. The controller implementation allows tunability of the PID gains over a large amplification and frequency range, while also providing precise control of the system and reproducibility of the gain parameters. By using the analog PID controller, we were able to perform successful atomic force microscopy imaging of a standard silicon calibration grating at line rates up to several kHz.

Optimized survey design for electrical resistivity tomography: combined optimization of measurement configuration and electrode placement

NASA Astrophysics Data System (ADS)

Uhlemann, Sebastian; Wilkinson, Paul B.; Maurer, Hansruedi; Wagner, Florian M.; Johnson, Timothy C.; Chambers, Jonathan E.

2018-07-01

Within geoelectrical imaging, the choice of measurement configurations and electrode locations is known to control the image resolution. Previous work has shown that optimized survey designs can provide a model resolution that is superior to standard survey designs. This paper demonstrates a methodology to optimize resolution within a target area, while limiting the number of required electrodes, thereby selecting optimal electrode locations. This is achieved by extending previous work on the `Compare-R' algorithm, which by calculating updates to the resolution matrix optimizes the model resolution in a target area. Here, an additional weighting factor is introduced that allows to preferentially adding measurement configurations that can be acquired on a given set of electrodes. The performance of the optimization is tested on two synthetic examples and verified with a laboratory study. The effect of the weighting factor is investigated using an acquisition layout comprising a single line of electrodes. The results show that an increasing weight decreases the area of improved resolution, but leads to a smaller number of electrode positions. Imaging results superior to a standard survey design were achieved using 56 per cent fewer electrodes. The performance was also tested on a 3-D acquisition grid, where superior resolution within a target at the base of an embankment was achieved using 22 per cent fewer electrodes than a comparable standard survey. The effect of the underlying resistivity distribution on the performance of the optimization was investigated and it was shown that even strong resistivity contrasts only have minor impact. The synthetic results were verified in a laboratory tank experiment, where notable image improvements were achieved. This work shows that optimized surveys can be designed that have a resolution superior to standard survey designs, while requiring significantly fewer electrodes. This methodology thereby provides a means for improving the efficiency of geoelectrical imaging.

Optimized survey design for Electrical Resistivity Tomography: combined optimization of measurement configuration and electrode placement

NASA Astrophysics Data System (ADS)

Uhlemann, Sebastian; Wilkinson, Paul B.; Maurer, Hansruedi; Wagner, Florian M.; Johnson, Timothy C.; Chambers, Jonathan E.

2018-03-01

Within geoelectrical imaging, the choice of measurement configurations and electrode locations is known to control the image resolution. Previous work has shown that optimized survey designs can provide a model resolution that is superior to standard survey designs. This paper demonstrates a methodology to optimize resolution within a target area, while limiting the number of required electrodes, thereby selecting optimal electrode locations. This is achieved by extending previous work on the `Compare-R' algorithm, which by calculating updates to the resolution matrix optimizes the model resolution in a target area. Here, an additional weighting factor is introduced that allows to preferentially adding measurement configurations that can be acquired on a given set of electrodes. The performance of the optimization is tested on two synthetic examples and verified with a laboratory study. The effect of the weighting factor is investigated using an acquisition layout comprising a single line of electrodes. The results show that an increasing weight decreases the area of improved resolution, but leads to a smaller number of electrode positions. Imaging results superior to a standard survey design were achieved using 56 per cent fewer electrodes. The performance was also tested on a 3D acquisition grid, where superior resolution within a target at the base of an embankment was achieved using 22 per cent fewer electrodes than a comparable standard survey. The effect of the underlying resistivity distribution on the performance of the optimization was investigated and it was shown that even strong resistivity contrasts only have minor impact. The synthetic results were verified in a laboratory tank experiment, where notable image improvements were achieved. This work shows that optimized surveys can be designed that have a resolution superior to standard survey designs, while requiring significantly fewer electrodes. This methodology thereby provides a means for improving the efficiency of geoelectrical imaging.

Enhanced speed in fluorescence imaging using beat frequency multiplexing

NASA Astrophysics Data System (ADS)

Mikami, Hideharu; Kobayashi, Hirofumi; Wang, Yisen; Hamad, Syed; Ozeki, Yasuyuki; Goda, Keisuke

2016-03-01

Fluorescence imaging using radiofrequency-tagged emission (FIRE) is an emerging technique that enables higher imaging speed (namely, temporal resolution) in fluorescence microscopy compared to conventional fluorescence imaging techniques such as confocal microscopy and wide-field microscopy. It works based on the principle that it uses multiple intensity-modulated fields in an interferometric setup as excitation fields and applies frequency-division multiplexing to fluorescence signals. Unfortunately, despite its high potential, FIRE has limited imaging speed due to two practical limitations: signal bandwidth and signal detection efficiency. The signal bandwidth is limited by that of an acousto-optic deflector (AOD) employed in the setup, which is typically 100-200 MHz for the spectral range of fluorescence excitation (400-600 nm). The signal detection efficiency is limited by poor spatial mode-matching between two interfering fields to produce a modulated excitation field. Here we present a method to overcome these limitations and thus to achieve higher imaging speed than the prior version of FIRE. Our method achieves an increase in signal bandwidth by a factor of two and nearly optimal mode matching, which enables the imaging speed limited by the lifetime of the target fluorophore rather than the imaging system itself. The higher bandwidth and better signal detection efficiency work synergistically because higher bandwidth requires higher signal levels to avoid the contribution of shot noise and amplifier noise to the fluorescence signal. Due to its unprecedentedly high-speed performance, our method has a wide variety of applications in cancer detection, drug discovery, and regenerative medicine.

Investigating the performance of reconstruction methods used in structured illumination microscopy as a function of the illumination pattern's modulation frequency

NASA Astrophysics Data System (ADS)

Shabani, H.; Sánchez-Ortiga, E.; Preza, C.

2016-03-01

Surpassing the resolution of optical microscopy defined by the Abbe diffraction limit, while simultaneously achieving optical sectioning, is a challenging problem particularly for live cell imaging of thick samples. Among a few developing techniques, structured illumination microscopy (SIM) addresses this challenge by imposing higher frequency information into the observable frequency band confined by the optical transfer function (OTF) of a conventional microscope either doubling the spatial resolution or filling the missing cone based on the spatial frequency of the pattern when the patterned illumination is two-dimensional. Standard reconstruction methods for SIM decompose the low and high frequency components from the recorded low-resolution images and then combine them to reach a high-resolution image. In contrast, model-based approaches rely on iterative optimization approaches to minimize the error between estimated and forward images. In this paper, we study the performance of both groups of methods by simulating fluorescence microscopy images from different type of objects (ranging from simulated two-point sources to extended objects). These simulations are used to investigate the methods' effectiveness on restoring objects with various types of power spectrum when modulation frequency of the patterned illumination is changing from zero to the incoherent cut-off frequency of the imaging system. Our results show that increasing the amount of imposed information by using a higher modulation frequency of the illumination pattern does not always yield a better restoration performance, which was found to be depended on the underlying object. Results from model-based restoration show performance improvement, quantified by an up to 62% drop in the mean square error compared to standard reconstruction, with increasing modulation frequency. However, we found cases for which results obtained with standard reconstruction methods do not always follow the same trend.

Data Assimilation and Predictability Studies on Typhoon Sinlaku (2008) Using the WRF-LETKF System

NASA Astrophysics Data System (ADS)

Miyoshi, T.; Kunii, M.

2011-12-01

Data assimilation and predictability studies on Tropical Cyclones with a particular focus on intensity forecasts are performed with the newly-developed Local Ensemble Transform Kalman Filter (LETKF) system with the WRF model. Taking advantage of intensive observations of the internationally collaborated T-PARC (THORPEX Pacific Asian Regional Campaign) project, we focus on Typhoon Sinlaku (2008) which intensified rapidly before making landfall to Taiwan. This study includes a number of data assimilation experiments, higher-resolution forecasts, and sensitivity analysis which quantifies impacts of observations on forecasts. This presentation includes latest achievements up to the time of the conference.

Numerical method for high accuracy index of refraction estimation for spectro-angular surface plasmon resonance systems.

PubMed

Alleyne, Colin J; Kirk, Andrew G; Chien, Wei-Yin; Charette, Paul G

2008-11-24

An eigenvector analysis based algorithm is presented for estimating refractive index changes from 2-D reflectance/dispersion images obtained with spectro-angular surface plasmon resonance systems. High resolution over a large dynamic range can be achieved simultaneously. The method performs well in simulations with noisy data maintaining an error of less than 10(-8) refractive index units with up to six bits of noise on 16 bit quantized image data. Experimental measurements show that the method results in a much higher signal to noise ratio than the standard 1-D weighted centroid dip finding algorithm.

Continuous decoding of human grasp kinematics using epidural and subdural signals

NASA Astrophysics Data System (ADS)

Flint, Robert D.; Rosenow, Joshua M.; Tate, Matthew C.; Slutzky, Marc W.

2017-02-01

Objective. Restoring or replacing function in paralyzed individuals will one day be achieved through the use of brain-machine interfaces. Regaining hand function is a major goal for paralyzed patients. Two competing prerequisites for the widespread adoption of any hand neuroprosthesis are accurate control over the fine details of movement, and minimized invasiveness. Here, we explore the interplay between these two goals by comparing our ability to decode hand movements with subdural and epidural field potentials (EFPs). Approach. We measured the accuracy of decoding continuous hand and finger kinematics during naturalistic grasping motions in five human subjects. We recorded subdural surface potentials (electrocorticography; ECoG) as well as with EFPs, with both standard- and high-resolution electrode arrays. Main results. In all five subjects, decoding of continuous kinematics significantly exceeded chance, using either EGoG or EFPs. ECoG decoding accuracy compared favorably with prior investigations of grasp kinematics (mean ± SD grasp aperture variance accounted for was 0.54 ± 0.05 across all subjects, 0.75 ± 0.09 for the best subject). In general, EFP decoding performed comparably to ECoG decoding. The 7-20 Hz and 70-115 Hz spectral bands contained the most information about grasp kinematics, with the 70-115 Hz band containing greater information about more subtle movements. Higher-resolution recording arrays provided clearly superior performance compared to standard-resolution arrays. Significance. To approach the fine motor control achieved by an intact brain-body system, it will be necessary to execute motor intent on a continuous basis with high accuracy. The current results demonstrate that this level of accuracy might be achievable not just with ECoG, but with EFPs as well. Epidural placement of electrodes is less invasive, and therefore may incur less risk of encephalitis or stroke than subdural placement of electrodes. Accurately decoding motor commands at the epidural level may be an important step towards a clinically viable brain-machine interface.

Continuous decoding of human grasp kinematics using epidural and subdural signals

PubMed Central

Flint, Robert D.; Rosenow, Joshua M.; Tate, Matthew C.; Slutzky, Marc W.

2017-01-01

Objective Restoring or replacing function in paralyzed individuals will one day be achieved through the use of brain-machine interfaces (BMIs). Regaining hand function is a major goal for paralyzed patients. Two competing prerequisites for the widespread adoption of any hand neuroprosthesis are: accurate control over the fine details of movement, and minimized invasiveness. Here, we explore the interplay between these two goals by comparing our ability to decode hand movements with subdural and epidural field potentials. Approach We measured the accuracy of decoding continuous hand and finger kinematics during naturalistic grasping motions in five human subjects. We recorded subdural surface potentials (electrocorticography; ECoG) as well as with epidural field potentials (EFPs), with both standard- and high-resolution electrode arrays. Main results In all five subjects, decoding of continuous kinematics significantly exceeded chance, using either EGoG or EFPs. ECoG decoding accuracy compared favorably with prior investigations of grasp kinematics (mean± SD grasp aperture variance accounted for was 0.54± 0.05 across all subjects, 0.75± 0.09 for the best subject). In general, EFP decoding performed comparably to ECoG decoding. The 7–20 Hz and 70–115 Hz spectral bands contained the most information about grasp kinematics, with the 70–115 Hz band containing greater information about more subtle movements. Higher-resolution recording arrays provided clearly superior performance compared to standard-resolution arrays. Significance To approach the fine motor control achieved by an intact brain-body system, it will be necessary to execute motor intent on a continuous basis with high accuracy. The current results demonstrate that this level of accuracy might be achievable not just with ECoG, but with EFPs as well. Epidural placement of electrodes is less invasive, and therefore may incur less risk of encephalitis or stroke than subdural placement of electrodes. Accurately decoding motor commands at the epidural level may be an important step towards a clinically viable brain-machine interface. PMID:27900947

Combining SMOS with visible and near/shortwave/thermal infrared satellite data for high resolution soil moisture estimates

NASA Astrophysics Data System (ADS)

Sánchez-Ruiz, Sergio; Piles, María; Sánchez, Nilda; Martínez-Fernández, José; Vall-llossera, Mercè; Camps, Adriano

2014-08-01

Sensors in the range of visible and near-shortwave-thermal infrared regions can be used in combination with passive microwave observations to provide soil moisture maps at much higher spatial resolution than the original resolution of current radiometers. To do so, a new downscaling algorithm ultimately based on the land surface temperature (LST) - Normalized Difference Vegetation Index (NDVI) - Brightness Temperature (TB) relationship is used, in which shortwave infrared indices are used as vegetation descriptors, instead of the more common near infrared ones. The theoretical basis of those indices, calculated as the normalized ratio of the 1240, 1640 and 2130 nm shortwave infrared (SWIR) bands and the 858 nm near infrared (NIR) band indicate that they are able to provide estimates of the vegetation water content. These so-called water indices extracted from MODIS products, have been used together with MODIS LST, and SMOS TB to improve the spatial resolution of ∼40 km SMOS soil moisture estimates. The aim was to retrieve soil moisture maps with the same accuracy as SMOS, but at the same resolution of the MODIS dataset, i.e., 500 m, which were then compared against in situ measurements from the REMEDHUS network in Spain. Results using two years of SMOS and MODIS data showed a similar performance for the four indices, with slightly better results when using the index derived from the first SWIR band. For the areal-average, a coefficient of correlation (R) of ∼0.61 and ∼0.72 for the morning and afternoon orbits, respectively, and a centered root mean square difference (cRMSD) of ∼0.04 m3 m-3 for both orbits was obtained. A twofold improvement of the current versions of this downscaling approach has been achieved by using more frequent and higher spatial resolution water indexes as vegetation descriptors: (1) the spatial resolution of the resulting soil moisture maps can be enhanced from ∼40 km up to 500 m, and (2) more accurate soil moisture maps (in terms of R and cRMSD) can be obtained, especially in periods of high vegetation activity. The results of this study support the use of high resolution LST and SWIR-based vegetation indices to disaggregate SMOS observations down to 500 m soil moisture maps, meeting the needs of fine-scale hydrological applications.

In vivo High Angular Resolution Diffusion-Weighted Imaging of Mouse Brain at 16.4 Tesla

PubMed Central

Alomair, Othman I.; Brereton, Ian M.; Smith, Maree T.; Galloway, Graham J.; Kurniawan, Nyoman D.

2015-01-01

Magnetic Resonance Imaging (MRI) of the rodent brain at ultra-high magnetic fields (> 9.4 Tesla) offers a higher signal-to-noise ratio that can be exploited to reduce image acquisition time or provide higher spatial resolution. However, significant challenges are presented due to a combination of longer T 1 and shorter T 2/T2* relaxation times and increased sensitivity to magnetic susceptibility resulting in severe local-field inhomogeneity artefacts from air pockets and bone/brain interfaces. The Stejskal-Tanner spin echo diffusion-weighted imaging (DWI) sequence is often used in high-field rodent brain MRI due to its immunity to these artefacts. To accurately determine diffusion-tensor or fibre-orientation distribution, high angular resolution diffusion imaging (HARDI) with strong diffusion weighting (b >3000 s/mm2) and at least 30 diffusion-encoding directions are required. However, this results in long image acquisition times unsuitable for live animal imaging. In this study, we describe the optimization of HARDI acquisition parameters at 16.4T using a Stejskal-Tanner sequence with echo-planar imaging (EPI) readout. EPI segmentation and partial Fourier encoding acceleration were applied to reduce the echo time (TE), thereby minimizing signal decay and distortion artefacts while maintaining a reasonably short acquisition time. The final HARDI acquisition protocol was achieved with the following parameters: 4 shot EPI, b = 3000 s/mm2, 64 diffusion-encoding directions, 125×150 μm2 in-plane resolution, 0.6 mm slice thickness, and 2h acquisition time. This protocol was used to image a cohort of adult C57BL/6 male mice, whereby the quality of the acquired data was assessed and diffusion tensor imaging (DTI) derived parameters were measured. High-quality images with high spatial and angular resolution, low distortion and low variability in DTI-derived parameters were obtained, indicating that EPI-DWI is feasible at 16.4T to study animal models of white matter (WM) diseases. PMID:26110770

Highly accelerated intracranial 4D flow MRI: evaluation of healthy volunteers and patients with intracranial aneurysms.

PubMed

Liu, Jing; Koskas, Louise; Faraji, Farshid; Kao, Evan; Wang, Yan; Haraldsson, Henrik; Kefayati, Sarah; Zhu, Chengcheng; Ahn, Sinyeob; Laub, Gerhard; Saloner, David

2018-04-01

To evaluate an accelerated 4D flow MRI method that provides high temporal resolution in a clinically feasible acquisition time for intracranial velocity imaging. Accelerated 4D flow MRI was developed by using a pseudo-random variable-density Cartesian undersampling strategy (CIRCUS) with the combination of k-t, parallel imaging and compressed sensing image reconstruction techniques (k-t SPARSE-SENSE). Four-dimensional flow data were acquired on five healthy volunteers and eight patients with intracranial aneurysms using CIRCUS (acceleration factor of R = 4, termed CIRCUS4) and GRAPPA (R = 2, termed GRAPPA2) as the reference method. Images with three times higher temporal resolution (R = 12, CIRCUS12) were also reconstructed from the same acquisition as CIRCUS4. Qualitative and quantitative image assessment was performed on the images acquired with different methods, and complex flow patterns in the aneurysms were identified and compared. Four-dimensional flow MRI with CIRCUS was achieved in 5 min and allowed further improved temporal resolution of <30 ms. Volunteer studies showed similar qualitative and quantitative evaluation obtained with the proposed approach compared to the reference (overall image scores: GRAPPA2 3.2 ± 0.6; CIRCUS4 3.1 ± 0.7; CIRCUS12 3.3 ± 0.4; difference of the peak velocities: -3.83 ± 7.72 cm/s between CIRCUS4 and GRAPPA2, -1.72 ± 8.41 cm/s between CIRCUS12 and GRAPPA2). In patients with intracranial aneurysms, the higher temporal resolution improved capturing of the flow features in intracranial aneurysms (pathline visualization scores: GRAPPA2 2.2 ± 0.2; CIRCUS4 2.5 ± 0.5; CIRCUS12 2.7 ± 0.6). The proposed rapid 4D flow MRI with a high temporal resolution is a promising tool for evaluating intracranial aneurysms in a clinically feasible acquisition time.

Replication of Muscle Cell Using Bioimprint

NASA Astrophysics Data System (ADS)

Samsuri, Fahmi; Mitchell, John S.; Alkaisi, Maan M.; Evans, John J.

2009-07-01

In our earlier study a heat-curable PDMS or a UV curable elastomer, was used as the replicating material to introduce Bioimprint methodology to facilitate cell imaging [1-2] But, replicating conditions for thermal polymerization is known to cause cell dehydration during curing. In this study, a new type of polymer was developed for use in living cell replica formation, and it was tested on human muscle cells. The cells were incubated and cultured according to standard biological culturing procedures, and they were grown for about 10 days. The replicas were then separated from the muscle cells and taken for analysis under an Atomic Force Microscope (AFM). The new polymer was designed to be biocompatible with higher resolution and fast curing process compared to other types of silicon-based organic polymers such as polydimethylsiloxane (PDMS). Muscle cell imprints were achieved and higher resolution images were able to show the micro structures of the muscle cells, including the cellular fibers and cell membranes. The AFM is able to image features at nanoscale resolution. This capacity enables a number of characteristics of biological cells to be visualized in a unique manner. Polymer and muscle cells preparations were developed at Hamilton, in collaboration between Plant and Food Research and the Department of Electrical and Computer Engineering, University of Canterbury. Tapping mode was used for the AFM image analysis as it has low tip-sample forces and non-destructive imaging capability. We will be presenting the bioimprinting processes of muscle cells, their AFM imaging and characterization of the newly developed polymer.

Water-perfused manometry vs three-dimensional high-resolution manometry: a comparative study on a large patient population with anorectal disorders.

PubMed

Vitton, V; Ben Hadj Amor, W; Baumstarck, K; Grimaud, J-C; Bouvier, M

2013-12-01

Our aim was to compare for the first time measurements obtained with water-perfused catheter anorectal manometry and three-dimensional (3D) high-resolution manometry in patients with anorectal disorders. Consecutive patients referred to our centre for anorectal manometry (ARM) were recruited to undergo the two procedures successively. Conventional manometry was carried out using a water-perfused catheter (WPAM) and high-resolution manometry was achieved with a 3D probe (3DHRAM). For each procedure, parameters recorded included the following: anal canal length, resting pressure, squeeze pressure and rectal sensitivity. Two hundred and one patients were included in this study. The mean values for resting and squeeze pressures were correlated and found to be significantly higher when measured with 3DHRAM than with WPAM. However, the length of the anal canal was not significantly different when measured by the two techniques without correlation between the two mean values obtained. The presence of the rectoanal inhibitory reflex was systematically assessed by both WPAM and 3DHRAM and anismus was also systematically diagnosed by both WPAM and 3DHRAM. The pressure values obtained with 3DHRAM are correlated with those measured with conventional manometry but are systematically higher. 3DHRAM has the advantage of providing a pressure recording over the entire length and circumference of the anal canal, allowing a more useful physiological assessment of anorectal function. Colorectal Disease © 2013 The Association of Coloproctology of Great Britain and Ireland.

Remote Sensing, GIS, and Vector-Borne Disease

NASA Technical Reports Server (NTRS)

Beck, Louisa R.

2001-01-01

The concept of global climate change encompasses more than merely an alteration in temperature; it also includes spatial and temporal covariations in precipitation and humidity, and more frequent occurrence of extreme weather events. The impact of these variations, which can occur at a variety of temporal and spatial scales, could have a direct impact on disease transmission through their environmental consequences for pathogen, vector, and host survival, as well as indirectly through human demographic and behavioral responses. New and future sensor systems will allow scientists to investigate the relationships between climate change and environmental risk factors at multiple spatial, temporal and spectral scales. Higher spatial resolution will provide better opportunities for mapping urban features previously only possible with high resolution aerial photography. These opportunities include housing quality (e.g., Chagas'disease, leishmaniasis) and urban mosquito habitats (e.g., dengue fever, filariasis, LaCrosse encephalitis). There are or will be many new sensors that have higher spectral resolution, enabling scientists to acquire more information about parameters such as soil moisture, soil type, better vegetation discrimination, and ocean color, to name a few. Although soil moisture content is now detectable using Landsat, the new thermal, shortwave infrared, and radar sensors will be able to provide this information at a variety of scales not achievable using Landsat. Soil moisture could become a key component in transmission risk models for Lyme disease (tick survival), helminthiases (worm habitat), malaria (vector-breeding habitat), and schistosomiasis (snail habitat).

TOT measurement implemented in FPGA TDC

NASA Astrophysics Data System (ADS)

Fan, Huan-Huan; Cao, Ping; Liu, Shu-Bin; An, Qi

2015-11-01

Time measurement plays a crucial role for the purpose of particle identification in high energy physics experiments. With increasingly demanding physics goals and the development of electronics, modern time measurement systems need to meet the requirement of excellent resolution specification as well as high integrity. Based on Field Programmable Gate Arrays (FPGAs), FPGA time-to-digital converters (TDCs) have become one of the most mature and prominent time measurement methods in recent years. For correcting the time-walk effect caused by leading timing, a time-over-threshold (TOT) measurement should be added to the FPGA TDC. TOT can be obtained by measuring the interval between the signal leading and trailing edges. Unfortunately, a traditional TDC can recognize only one kind of signal edge, the leading or the trailing. Generally, to measure the interval, two TDC channels need to be used at the same time, one for leading, the other for trailing. However, this method unavoidably increases the amount of FPGA resources used and reduces the TDC's integrity. This paper presents one method of TOT measurement implemented in a Xilinx Virtex-5 FPGA. In this method, TOT measurement can be achieved using only one TDC input channel. The consumed resources and time resolution can both be guaranteed. Testing shows that this TDC can achieve resolution better than 15ps for leading edge measurement and 37 ps for TOT measurement. Furthermore, the TDC measurement dead time is about two clock cycles, which makes it good for applications with higher physics event rates. Supported by National Natural Science Foundation of China (11079003, 10979003)

Characterization of Harmonic Signal Acquisition with Parallel Dipole and Multipole Detectors

NASA Astrophysics Data System (ADS)

Park, Sung-Gun; Anderson, Gordon A.; Bruce, James E.

2018-04-01

Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) is a powerful instrument for the study of complex biological samples due to its high resolution and mass measurement accuracy. However, the relatively long signal acquisition periods needed to achieve high resolution can serve to limit applications of FTICR-MS. The use of multiple pairs of detector electrodes enables detection of harmonic frequencies present at integer multiples of the fundamental cyclotron frequency, and the obtained resolving power for a given acquisition period increases linearly with the order of harmonic signal. However, harmonic signal detection also increases spectral complexity and presents challenges for interpretation. In the present work, ICR cells with independent dipole and harmonic detection electrodes and preamplifiers are demonstrated. A benefit of this approach is the ability to independently acquire fundamental and multiple harmonic signals in parallel using the same ions under identical conditions, enabling direct comparison of achieved performance as parameters are varied. Spectra from harmonic signals showed generally higher resolving power than spectra acquired with fundamental signals and equal signal duration. In addition, the maximum observed signal to noise (S/N) ratio from harmonic signals exceeded that of fundamental signals by 50 to 100%. Finally, parallel detection of fundamental and harmonic signals enables deconvolution of overlapping harmonic signals since observed fundamental frequencies can be used to unambiguously calculate all possible harmonic frequencies. Thus, the present application of parallel fundamental and harmonic signal acquisition offers a general approach to improve utilization of harmonic signals to yield high-resolution spectra with decreased acquisition time. [Figure not available: see fulltext.

A multi-channel gel electrophoresis and continuous fraction collection apparatus for high throughput protein separation and characterization

DOE Office of Scientific and Technical Information (OSTI.GOV)

Choi, Megan; Nordmeyer, Robert A.; Cornell, Earl

2009-10-02

To facilitate a direct interface between protein separation by PAGE and protein identification by mass spectrometry, we developed a multichannel system that continuously collects fractions as protein bands migrate off the bottom of gel electrophoresis columns. The device was constructed using several short linear gel columns, each of a different percent acrylamide, to achieve a separation power similar to that of a long gradient gel. A Counter Free-Flow elution technique then allows continuous and simultaneous fraction collection from multiple channels at low cost. We demonstrate that rapid, high-resolution separation of a complex protein mixture can be achieved on this systemmore » using SDS-PAGE. In a 2.5 h electrophoresis run, for example, each sample was separated and eluted into 48-96 fractions over a mass range of 10-150 kDa; sample recovery rates were 50percent or higher; each channel was loaded with up to 0.3 mg of protein in 0.4 mL; and a purified band was eluted in two to three fractions (200 L/fraction). Similar results were obtained when running native gel electrophoresis, but protein aggregation limited the loading capacity to about 50 g per channel and reduced resolution.« less

Covered self-expandable metal stents for benign biliary tract diseases.

PubMed

Baron, Todd H

2011-05-01

Benign biliary diseases are often managed endoscopically using plastic stents. Benign biliary strictures (BBS) respond to placement of multiple large-bore plastic stents, though requiring multiple procedures to place stents, and to exchange stents to prevent and/or treat stent occlusion. Bile leaks close using plastic stents, which divert bile away from the leak into the duodenum. Covered self-expandable metal stents (CSEMS), intended for palliation of malignant biliary obstruction, have been used to treat benign biliary diseases. Advantages include small predeployment and large postexpansion diameters. Lack of imbedding of the metal into the bile duct wall enables removability. For strictures, one CSEMS is inserted without need for dilation and remains in place for up to 6 months. Successful removal has been reported in all cases. Long-term stricture resolution is achieved in up to 92%. Adverse events include migration and new stricture formation. For treatment of complex bile leaks, the covering and large diameter allow successful closure in nearly all cases. Other uses of CSEMS include treatment of postsphincterotomy bleeding and closure of perforations. CSEMS show promise for treatment of BBS and complex biliary leaks. Successful resolution can be achieved in the majority of patients with the advantage of fewer procedures, which offsets their higher cost.

Parametric Study for Increasing On-Station Duration via Unconventional Aircraft Launch Approach

NASA Technical Reports Server (NTRS)

Kuhl, Christopher A.; Moses, Robert W.; Croom, Mark A.; Sandford, Stephen P.

2004-01-01

The need for better atmospheric predictions is causing the atmospheric science community to look for new ways to obtain longer, higher-resolution measurements over several diurnal cycles. The high resolution, in-situ measurements required to study many atmospheric phenomena can be achieved by an Autonomous Aerial Observation System (AAOS); however, meeting the long on-station time requirements with an aerial platform poses many challenges. Inspired by the half-scale drop test of the deployable Aerial Regional-scale Environmental Survey (ARES) Mars airplane, a study was conducted at the NASA Langley Research Center to examine the possibility of increasing on-station time by launching an airplane directly at the desired altitude. The ARES Mars airplane concept was used as a baseline for Earth atmospheric flight, and parametric analyses of fundamental configuration elements were performed to study their impact on achieving desired on-station time with this class of airplane. The concept involved lifting the aircraft from the ground to the target altitude by means of an air balloon, thereby unburdening the airplane of ascent requirements. The parameters varied in the study were aircraft wingspan, payload, fuel quantity, and propulsion system. The results show promising trends for further research into aircraft-payload design using this unconventional balloon-based launch approach.

Image scanning fluorescence emission difference microscopy based on a detector array.

PubMed

Li, Y; Liu, S; Liu, D; Sun, S; Kuang, C; Ding, Z; Liu, X

2017-06-01

We propose a novel imaging method that enables the enhancement of three-dimensional resolution of confocal microscopy significantly and achieve experimentally a new fluorescence emission difference method for the first time, based on the parallel detection with a detector array. Following the principles of photon reassignment in image scanning microscopy, images captured by the detector array were arranged. And by selecting appropriate reassign patterns, the imaging result with enhanced resolution can be achieved with the method of fluorescence emission difference. Two specific methods are proposed in this paper, showing that the difference between an image scanning microscopy image and a confocal image will achieve an improvement of transverse resolution by approximately 43% compared with that in confocal microscopy, and the axial resolution can also be enhanced by at least 22% experimentally and 35% theoretically. Moreover, the methods presented in this paper can improve the lateral resolution by around 10% than fluorescence emission difference and 15% than Airyscan. The mechanism of our methods is verified by numerical simulations and experimental results, and it has significant potential in biomedical applications. © 2017 The Authors Journal of Microscopy © 2017 Royal Microscopical Society.

Obtaining high resolution XUV coronal images

NASA Technical Reports Server (NTRS)

Golub, L.; Spiller, E.

1992-01-01

Photographs obtained during three flights of an 11 inch diameter normal incident soft X-ray (wavelength 63.5 A) telescope are analyzed and the data are compared to the results expected from tests of the mirror surfaces. Multilayer coated X ray telescopes have the potential for 0.01 arcsec resolution, and there is optimism that such high quality mirrors can be built. Some of the factors which enter into the performance actually achieved in practice are as follows: quality of the mirror substrate, quality of the multilayer coating, and number of photons collected. Measurements of multilayer mirrors show that the actual performance achieved in the solar X-ray images demonstrates a reduction in the scattering compared to that calculated from the topography of the top surface of the multilayer. In the brief duration of a rocket flight, the resolution is also limited by counting statistics from the number of photons collected. At X-ray Ultraviolet (XUV) wavelengths from 171 to 335 A the photon flux should be greater than 10(exp 10) ph/sec, so that a resolution better than 0.1 arcsec might be achieved, if mirror quality does not provide a limit first. In a satellite, a large collecting area will be needed for the highest resolution.

Developing a CCD camera with high spatial resolution for RIXS in the soft X-ray range

NASA Astrophysics Data System (ADS)

Soman, M. R.; Hall, D. J.; Tutt, J. H.; Murray, N. J.; Holland, A. D.; Schmitt, T.; Raabe, J.; Schmitt, B.

2013-12-01

The Super Advanced X-ray Emission Spectrometer (SAXES) at the Swiss Light Source contains a high resolution Charge-Coupled Device (CCD) camera used for Resonant Inelastic X-ray Scattering (RIXS). Using the current CCD-based camera system, the energy-dispersive spectrometer has an energy resolution (E/ΔE) of approximately 12,000 at 930 eV. A recent study predicted that through an upgrade to the grating and camera system, the energy resolution could be improved by a factor of 2. In order to achieve this goal in the spectral domain, the spatial resolution of the CCD must be improved to better than 5 μm from the current 24 μm spatial resolution (FWHM). The 400 eV-1600 eV energy X-rays detected by this spectrometer primarily interact within the field free region of the CCD, producing electron clouds which will diffuse isotropically until they reach the depleted region and buried channel. This diffusion of the charge leads to events which are split across several pixels. Through the analysis of the charge distribution across the pixels, various centroiding techniques can be used to pinpoint the spatial location of the X-ray interaction to the sub-pixel level, greatly improving the spatial resolution achieved. Using the PolLux soft X-ray microspectroscopy endstation at the Swiss Light Source, a beam of X-rays of energies from 200 eV to 1400 eV can be focused down to a spot size of approximately 20 nm. Scanning this spot across the 16 μm square pixels allows the sub-pixel response to be investigated. Previous work has demonstrated the potential improvement in spatial resolution achievable by centroiding events in a standard CCD. An Electron-Multiplying CCD (EM-CCD) has been used to improve the signal to effective readout noise ratio achieved resulting in a worst-case spatial resolution measurement of 4.5±0.2 μm and 3.9±0.1 μm at 530 eV and 680 eV respectively. A method is described that allows the contribution of the X-ray spot size to be deconvolved from these worst-case resolution measurements, estimating the spatial resolution to be approximately 3.5 μm and 3.0 μm at 530 eV and 680 eV, well below the resolution limit of 5 μm required to improve the spectral resolution by a factor of 2.

High-Resolution Large Field-of-View FUV Compact Camera

NASA Technical Reports Server (NTRS)

Spann, James F.

2006-01-01

The need for a high resolution camera with a large field of view and capable to image dim emissions in the far-ultraviolet is driven by the widely varying intensities of FUV emissions and spatial/temporal scales of phenomena of interest in the Earth% ionosphere. In this paper, the concept of a camera is presented that is designed to achieve these goals in a lightweight package with sufficient visible light rejection to be useful for dayside and nightside emissions. The camera employs the concept of self-filtering to achieve good spectral resolution tuned to specific wavelengths. The large field of view is sufficient to image the Earth's disk at Geosynchronous altitudes and capable of a spatial resolution of >20 km. The optics and filters are emphasized.

ICA-based artefact and accelerated fMRI acquisition for improved Resting State Network imaging

PubMed Central

Griffanti, Ludovica; Salimi-Khorshidi, Gholamreza; Beckmann, Christian F.; Auerbach, Edward J.; Douaud, Gwenaëlle; Sexton, Claire E.; Zsoldos, Enikő; Ebmeier, Klaus P; Filippini, Nicola; Mackay, Clare E.; Moeller, Steen; Xu, Junqian; Yacoub, Essa; Baselli, Giuseppe; Ugurbil, Kamil; Miller, Karla L.; Smith, Stephen M.

2014-01-01

The identification of resting state networks (RSNs) and the quantification of their functional connectivity in resting-state fMRI (rfMRI) are seriously hindered by the presence of artefacts, many of which overlap spatially or spectrally with RSNs. Moreover, recent developments in fMRI acquisition yield data with higher spatial and temporal resolutions, but may increase artefacts both spatially and/or temporally. Hence the correct identification and removal of non-neural fluctuations is crucial, especially in accelerated acquisitions. In this paper we investigate the effectiveness of three data-driven cleaning procedures, compare standard against higher (spatial and temporal) resolution accelerated fMRI acquisitions, and investigate the combined effect of different acquisitions and different cleanup approaches. We applied single-subject independent component analysis (ICA), followed by automatic component classification with FMRIB’s ICA-based X-noiseifier (FIX) to identify artefactual components. We then compared two first-level (within-subject) cleaning approaches for removing those artefacts and motion-related fluctuations from the data. The effectiveness of the cleaning procedures were assessed using timeseries (amplitude and spectra), network matrix and spatial map analyses. For timeseries and network analyses we also tested the effect of a second-level cleaning (informed by group-level analysis). Comparing these approaches, the preferable balance between noise removal and signal loss was achieved by regressing out of the data the full space of motion-related fluctuations and only the unique variance of the artefactual ICA components. Using similar analyses, we also investigated the effects of different cleaning approaches on data from different acquisition sequences. With the optimal cleaning procedures, functional connectivity results from accelerated data were statistically comparable or significantly better than the standard (unaccelerated) acquisition, and, crucially, with higher spatial and temporal resolution. Moreover, we were able to perform higher dimensionality ICA decompositions with the accelerated data, which is very valuable for detailed network analyses. PMID:24657355

ICA-based artefact removal and accelerated fMRI acquisition for improved resting state network imaging.

PubMed

Griffanti, Ludovica; Salimi-Khorshidi, Gholamreza; Beckmann, Christian F; Auerbach, Edward J; Douaud, Gwenaëlle; Sexton, Claire E; Zsoldos, Enikő; Ebmeier, Klaus P; Filippini, Nicola; Mackay, Clare E; Moeller, Steen; Xu, Junqian; Yacoub, Essa; Baselli, Giuseppe; Ugurbil, Kamil; Miller, Karla L; Smith, Stephen M

2014-07-15

The identification of resting state networks (RSNs) and the quantification of their functional connectivity in resting-state fMRI (rfMRI) are seriously hindered by the presence of artefacts, many of which overlap spatially or spectrally with RSNs. Moreover, recent developments in fMRI acquisition yield data with higher spatial and temporal resolutions, but may increase artefacts both spatially and/or temporally. Hence the correct identification and removal of non-neural fluctuations is crucial, especially in accelerated acquisitions. In this paper we investigate the effectiveness of three data-driven cleaning procedures, compare standard against higher (spatial and temporal) resolution accelerated fMRI acquisitions, and investigate the combined effect of different acquisitions and different cleanup approaches. We applied single-subject independent component analysis (ICA), followed by automatic component classification with FMRIB's ICA-based X-noiseifier (FIX) to identify artefactual components. We then compared two first-level (within-subject) cleaning approaches for removing those artefacts and motion-related fluctuations from the data. The effectiveness of the cleaning procedures was assessed using time series (amplitude and spectra), network matrix and spatial map analyses. For time series and network analyses we also tested the effect of a second-level cleaning (informed by group-level analysis). Comparing these approaches, the preferable balance between noise removal and signal loss was achieved by regressing out of the data the full space of motion-related fluctuations and only the unique variance of the artefactual ICA components. Using similar analyses, we also investigated the effects of different cleaning approaches on data from different acquisition sequences. With the optimal cleaning procedures, functional connectivity results from accelerated data were statistically comparable or significantly better than the standard (unaccelerated) acquisition, and, crucially, with higher spatial and temporal resolution. Moreover, we were able to perform higher dimensionality ICA decompositions with the accelerated data, which is very valuable for detailed network analyses. Copyright © 2014 Elsevier Inc. All rights reserved.

CO2-Tea pulse clipping using pulsed high voltage preionization for high spatial resolution I.R. Lidar systems

NASA Astrophysics Data System (ADS)

Gasmi, Taieb

2018-04-01

An extra-cavity CO2-TEA laser pulse clipper for high spatial resolution atmospheric monitoring is presented. The clipper uses pulsed high voltageto facilitate the breakdown of the gas within the clipper cell. Complete extinction of the nitrogen tail, that degrades the range resolution of LIDARS, is obtained at pressures from 375 up to 1500 Torr for nitrogen and argon gases whereas an attenuation coefficient of almost 102 is achieved for helium. Excellent energy stability and pulse width repeatability were achieved using high voltage pre-ionized gas technique.

Measuring true localization accuracy in super resolution microscopy with DNA-origami nanostructures

NASA Astrophysics Data System (ADS)

Reuss, Matthias; Fördős, Ferenc; Blom, Hans; Öktem, Ozan; Högberg, Björn; Brismar, Hjalmar

2017-02-01

A common method to assess the performance of (super resolution) microscopes is to use the localization precision of emitters as an estimate for the achieved resolution. Naturally, this is widely used in super resolution methods based on single molecule stochastic switching. This concept suffers from the fact that it is hard to calibrate measures against a real sample (a phantom), because true absolute positions of emitters are almost always unknown. For this reason, resolution estimates are potentially biased in an image since one is blind to true position accuracy, i.e. deviation in position measurement from true positions. We have solved this issue by imaging nanorods fabricated with DNA-origami. The nanorods used are designed to have emitters attached at each end in a well-defined and highly conserved distance. These structures are widely used to gauge localization precision. Here, we additionally determined the true achievable localization accuracy and compared this figure of merit to localization precision values for two common super resolution microscope methods STED and STORM.

Achieving subpixel resolution with time-correlated transient signals in pixelated CdZnTe gamma-ray sensors using a focused laser beam (Conference Presentation)

NASA Astrophysics Data System (ADS)

Ocampo Giraldo, Luis A.; Bolotnikov, Aleksey E.; Camarda, Giuseppe S.; Cui, Yonggang; De Geronimo, Gianluigi; Gul, Rubi; Fried, Jack; Hossain, Anwar; Unlu, Kenan; Vernon, Emerson; Yang, Ge; James, Ralph B.

2017-05-01

High-resolution position-sensitive detectors have been proposed to correct response non-uniformities in Cadmium Zinc Telluride (CZT) crystals by virtually subdividing the detectors area into small voxels and equalizing responses from each voxel. 3D pixelated detectors coupled with multichannel readout electronics are the most advanced type of CZT devices offering many options in signal processing and enhancing detector performance. One recent innovation proposed for pixelated detectors is to use the induced (transient) signals from neighboring pixels to achieve high sub-pixel position resolution while keeping large pixel sizes. The main hurdle in achieving this goal is the relatively low signal induced on the neighboring pixels because of the electrostatic shielding effect caused by the collecting pixel. In addition, to achieve high position sensitivity one should rely on time-correlated transient signals, which means that digitized output signals must be used. We present the results of our studies to measure the amplitude of the pixel signals so that these can be used to measure positions of the interaction points. This is done with the processing of digitized correlated time signals measured from several adjacent pixels taking into account rise-time and charge-sharing effects. In these measurements we used a focused pulsed laser to generate a 10-micron beam at one milliwatt (650-nm wavelength) over the detector surface while the collecting pixel was moved in cardinal directions. The results include measurements that present the benefits of combining conventional pixel geometry with digital pulse processing for the best approach in achieving sub-pixel position resolution with the pixel dimensions of approximately 2 mm. We also present the sub-pixel resolution measurements at comparable energies from various gamma emitting isotopes.

The CHARIS High-Contrast Integral-Field Spectrograph

NASA Technical Reports Server (NTRS)

Groff, Tyler D.; Chilcote, Jeffrey; Brandt, Timothy; Kasdin, N. Jeremy; Galvin, Michael; Loomis, Craig; Rizzo, Maxime; Knapp, Gillian; Guyon, Olivier; Jovanovic, Nemanja;

Adolescents', mothers', and fathers' gendered coping strategies during conflict: Youth and parent influences on conflict resolution and psychopathology.

PubMed

Marceau, Kristine; Zahn-Waxler, Carolyn; Shirtcliff, Elizabeth A; Schreiber, Jane E; Hastings, Paul; Klimes-Dougan, Bonnie

2015-11-01

We observed gendered coping strategies and conflict resolution outcomes used by adolescents and parents during a conflict discussion task to evaluate associations with current and later adolescent psychopathology. We studied 137 middle- to upper-middle-class, predominantly Caucasian families of adolescents (aged 11-16 years, 65 males) who represented a range of psychological functioning, including normative, subclinical, and clinical levels of problems. Adolescent coping strategies played key roles both in the extent to which parent-adolescent dyads resolved conflict and in the trajectory of psychopathology symptom severity over a 2-year period. Gender-prototypic adaptive coping strategies were observed in parents but not youth, (i.e., more problem solving by fathers than mothers and more regulated emotion-focused coping by mothers than fathers). Youth-mother dyads more often achieved full resolution of conflict than youth-father dyads. There were generally not bidirectional effects among youth and parents' coping across the discussion except boys' initial use of angry/hostile coping predicted fathers' angry/hostile coping. The child was more influential than the parent on conflict resolution. This extended to exacerbation/alleviation of psychopathology over 2 years: higher conflict resolution mediated the association of adolescents' use of problem-focused coping with decreases in symptom severity over time. Lower conflict resolution mediated the association of adolescents' use of angry/hostile emotion coping with increases in symptom severity over time. Implications of findings are considered within a broadened context of the nature of coping and conflict resolution in youth-parent interactions, as well as on how these processes impact youth well-being and dysfunction over time.

Adolescents’, Mothers’, and Fathers’ Gendered Coping Strategies during Conflict: Youth and Parent Influences on Conflict Resolution and Psychopathology

PubMed Central

Marceau, Kristine; Zahn-Waxler, Carolyn; Shirtcliff, Elizabeth A.; Schreiber, Jane E; Hastings, Paul; Klimes-Dougan, Bonnie

2015-01-01

We observed gendered coping strategies and conflict resolution outcomes used by adolescents and parents during a conflict discussion task to evaluate associations with current and later adolescent psychopathology. We studied 137 middle-to-upper-middle class predominantly Caucasian families of adolescents (aged 11–16 years, 65 males) who represented a range of psychological functioning including normative (~1/3) sub-clinical (~1/3) and clinical (~1/3) levels of problems. Adolescent coping strategies played key roles both in the extent to which parent-adolescent dyads resolved conflict and in the trajectory of psychopathology symptom severity over a two-year period. Gender-prototypic adaptive coping strategies were observed in parents but not youth, i.e. more problem-solving by fathers than mothers and more regulated emotion-focused coping by mothers than fathers. Youth-mother dyads more often achieved full resolution of conflict than youth-father dyads. There were generally not bidirectional effects among youth and parents’ coping across the discussion except boys’ initial use of angry/hostile coping predicted fathers’ angry/hostile coping. The child was more influential than the parent on conflict resolution. This extended to exacerbation/alleviation of psychopathology over two years: higher conflict resolution mediated the association of adolescents’ use of problem-focused coping with decreases in symptom severity over time. Lower conflict resolution mediated the association of adolescents’ use of angry/hostile emotion coping with increases in symptom severity over time. Implications of findings are considered within a broadened context of the nature of coping and conflict resolution in youth-parent interactions, as well as how these processes impact on youth well-being and dysfunction over time. PMID:26439060

Differential Deposition Technique for Figure Corrections in Grazing Incidence X-ray Optics

NASA Technical Reports Server (NTRS)

Kilaru, Kiranmayee; Ramsey, Brian D.; Gubarev, Mikhail

2009-01-01

A differential deposition technique is being developed to correct the low- and mid-spatial-frequency deviations in the axial figure profile of Wolter type grazing incidence X-ray optics. These deviations arise due to various factors in the fabrication process and they degrade the performance of the optics by limiting the achievable angular resolution. In the differential deposition technique, material of varying thickness is selectively deposited along the length of the optic to minimize these deviations, thereby improving the overall figure. High resolution focusing optics being developed at MSFC for small animal radionuclide imaging are being coated to test the differential deposition technique. The required spatial resolution for these optics is 100 m. This base resolution is achievable with the regular electroform-nickel-replication fabrication technique used at MSFC. However, by improving the figure quality of the optics through differential deposition, we aim at significantly improving the resolution beyond this value.

Super-Resolution Scanning Laser Microscopy Based on Virtually Structured Detection

PubMed Central

Zhi, Yanan; Wang, Benquan; Yao, Xincheng

2016-01-01

Light microscopy plays a key role in biological studies and medical diagnosis. The spatial resolution of conventional optical microscopes is limited to approximately half the wavelength of the illumination light as a result of the diffraction limit. Several approaches—including confocal microscopy, stimulated emission depletion microscopy, stochastic optical reconstruction microscopy, photoactivated localization microscopy, and structured illumination microscopy—have been established to achieve super-resolution imaging. However, none of these methods is suitable for the super-resolution ophthalmoscopy of retinal structures because of laser safety issues and inevitable eye movements. We recently experimentally validated virtually structured detection (VSD) as an alternative strategy to extend the diffraction limit. Without the complexity of structured illumination, VSD provides an easy, low-cost, and phase artifact–free strategy to achieve super-resolution in scanning laser microscopy. In this article we summarize the basic principles of the VSD method, review our demonstrated single-point and line-scan super-resolution systems, and discuss both technical challenges and the potential of VSD-based instrumentation for super-resolution ophthalmoscopy of the retina. PMID:27480461

DOE Office of Scientific and Technical Information (OSTI.GOV)

Naulleau, Patrick

With demonstrated resist resolution of 20 nm half pitch, the SEMATECH Berkeley BUV microfield exposure tool continues to push crucial advances in the areas of BUY resists and masks. The ever progressing shrink in computer chip feature sizes has been fueled over the years by a continual reduction in the wavelength of light used to pattern the chips. Recently, this trend has been threatened by unavailability of lens materials suitable for wavelengths shorter than 193 nm. To circumvent this roadblock, a reflective technology utilizing a significantly shorter extreme ultraviolet (EUV) wavelength (13.5 nm) has been under development for the pastmore » decade. The dramatic wavelength shrink was required to compensate for optical design limitations intrinsic in mirror-based systems compared to refractive lens systems. With this significant reduction in wavelength comes a variety of new challenges including developing sources of adequate power, photoresists with suitable resolution, sensitivity, and line-edge roughness characteristics, as well as the fabrication of reflection masks with zero defects. While source development can proceed in the absence of available exposure tools, in order for progress to be made in the areas of resists and masks it is crucial to have access to advanced exposure tools with resolutions equal to or better than that expected from initial production tools. These advanced development tools, however, need not be full field tools. Also, implementing such tools at synchrotron facilities allows them to be developed independent of the availability of reliable stand-alone BUY sources. One such tool is the SEMATECH Berkeley microfield exposure tool (MET). The most unique attribute of the SEMA TECH Berkeley MET is its use of a custom-coherence illuminator made possible by its implementation on a synchrotron beamline. With only conventional illumination and conventional binary masks, the resolution limit of the 0.3-NA optic is approximately 25 nm, however, with EUV not expected in production before the 22-nm half pitch node even finer resolution capabilities are now required from development tools. The SEMATECH Berkeley MET's custom-coherence illuminator allows it to be used with aggressive modified illumination enabling kJ factors as low as 0.25. Noting that the lithographic resolution of an exposure tool is defined as k{sub 1}{lambda}/NA, yielding an ultimate resolution limit of 11 nm. To achieve sub-20-nm aerial-image resolution while avoiding forbidden pitches on Manhattan-geometry features with the centrally-obscured MET optic, a 45-degree oriented dipole pupil fill is used. Figure 1 shows the computed aerial-image contrast as a function of half pitch for a dipole pupil fill optimized to print down to the 19-nm half pitch level. This is achieved with relatively uniform performance at larger dimensions. Using this illumination, printing down to the 20-nm half pitch level has been demonstrated in chemically amplified resists as shown in Fig. 2. The SEMATECH Berkeley MET tool plays a crucial role in the advancement of EUV resists. The unique programmable coherence properties of this tool enable it to achieve higher resolution than other EUV projection tools. As presented here, over the past year the tool has been used to demonstrate resist resolutions of 20 half pitch. Although not discussed here, because the Berkeley MET tool is a true projection lithography tool, it also plays a crucial role in advanced EUV mask research. Examples of the work done in this area include defect printability, mask architecture, and phase shift masks.« less

Polystyrene negative resist for high-resolution electron beam lithography

PubMed Central

2011-01-01

We studied the exposure behavior of low molecular weight polystyrene as a negative tone electron beam lithography (EBL) resist, with the goal of finding the ultimate achievable resolution. It demonstrated fairly well-defined patterning of a 20-nm period line array and a 15-nm period dot array, which are the densest patterns ever achieved using organic EBL resists. Such dense patterns can be achieved both at 20 and 5 keV beam energies using different developers. In addition to its ultra-high resolution capability, polystyrene is a simple and low-cost resist with easy process control and practically unlimited shelf life. It is also considerably more resistant to dry etching than PMMA. With a low sensitivity, it would find applications where negative resist is desired and throughput is not a major concern. PMID:21749679

3.0-T functional brain imaging: a 5-year experience.

PubMed

Scarabino, T; Giannatempo, G M; Popolizio, T; Tosetti, M; d'Alesio, V; Esposito, F; Di Salle, F; Di Costanzo, A; Bertolino, A; Maggialetti, A; Salvolini, U

2007-02-01

The aim of this paper is to illustrate the technical, methodological and diagnostic features of functional imaging (comprising spectroscopy, diffusion, perfusion and cortical activation techniques) and its principal neuroradiological applications on the basis of the experience gained by the authors in the 5 years since the installation of a high-field magnetic resonance (MR) magnet. These MR techniques are particularly effective at 3.0 Tesla (T) owing to their high signal, resolution and sensitivity, reduced scanning times and overall improved diagnostic ability. In particular, the high-field strength enhances spectroscopic analysis due to a greater signal-to-noise ratio (SNR) and improved spectral, space and time resolution, resulting in the ability to obtain high-resolution spectroscopic studies not only of the more common metabolites, but also--and especially--of those which, due to their smaller concentrations, are difficult to detect using 1.5-T systems. All of these advantages can be obtained with reduced acquisition times. In diffusion studies, the high-field strength results in greater SNR, because 3.0-T magnets enable increased spatial resolution, which enhances accuracy. They also allow exploration in greater detail of more complex phenomena (such as diffusion tensor and tractography), which are not clearly depicted on 1.5-T systems. The most common perfusion study (with intravenous injection of a contrast agent) benefits from the greater SNR and higher magnetic susceptibility by achieving dramatically improved signal changes, and thus greater reliability, using smaller doses of contrast agent. Functional MR imaging (fMRI) is without doubt the modality in which high-field strength has had the greatest impact. Images acquired with the blood-oxygen-level-dependent (BOLD) technique benefit from the greater SNR afforded by 3.0-T magnets and from their stronger magnetic susceptibility effects, providing higher signal and spatial resolution. This enhances reliability of the localisation of brain functions, making it possible to map additional areas, even in the millimetre and submillimetre scale. The data presented and results obtained to date show that 3.0-T morphofunctional imaging can become the standard for high-resolution investigation of brain disease.

Single-shot spiral imaging at 7 T.

PubMed

Engel, Maria; Kasper, Lars; Barmet, Christoph; Schmid, Thomas; Vionnet, Laetitia; Wilm, Bertram; Pruessmann, Klaas P

2018-03-25

The purpose of this work is to explore the feasibility and performance of single-shot spiral MRI at 7 T, using an expanded signal model for reconstruction. Gradient-echo brain imaging is performed on a 7 T system using high-resolution single-shot spiral readouts and half-shot spirals that perform dual-image acquisition after a single excitation. Image reconstruction is based on an expanded signal model including the encoding effects of coil sensitivity, static off-resonance, and magnetic field dynamics. The latter are recorded concurrently with image acquisition, using NMR field probes. The resulting image resolution is assessed by point spread function analysis. Single-shot spiral imaging is achieved at a nominal resolution of 0.8 mm, using spiral-out readouts of 53-ms duration. High depiction fidelity is achieved without conspicuous blurring or distortion. Effective resolutions are assessed as 0.8, 0.94, and 0.98 mm in CSF, gray matter and white matter, respectively. High image quality is also achieved with half-shot acquisition yielding image pairs at 1.5-mm resolution. Use of an expanded signal model enables single-shot spiral imaging at 7 T with unprecedented image quality. Single-shot and half-shot spiral readouts deploy the sensitivity benefit of high field for rapid high-resolution imaging, particularly for functional MRI and arterial spin labeling. © 2018 International Society for Magnetic Resonance in Medicine.

Feasibility of high-resolution one-dimensional relaxation imaging at low magnetic field using a single-sided NMR scanner applied to articular cartilage.

PubMed

Rössler, Erik; Mattea, Carlos; Stapf, Siegfried

2015-02-01

Low field Nuclear Magnetic Resonance increases the contrast of the longitudinal relaxation rate in many biological tissues; one prominent example is hyaline articular cartilage. In order to take advantage of this increased contrast and to profile the depth-dependent variations, high resolution parameter measurements are carried out which can be of critical importance in an early diagnosis of cartilage diseases such as osteoarthritis. However, the maximum achievable spatial resolution of parameter profiles is limited by factors such as sensor geometry, sample curvature, and diffusion limitation. In this work, we report on high-resolution single-sided NMR scanner measurements with a commercial device, and quantify these limitations. The highest achievable spatial resolution on the used profiler, and the lateral dimension of the sensitive volume were determined. Since articular cartilage samples are usually bent, we also focus on averaging effects inside the horizontally aligned sensitive volume and their impact on the relaxation profiles. Taking these critical parameters into consideration, depth-dependent relaxation time profiles with the maximum achievable vertical resolution of 20 μm are discussed, and are correlated with diffusion coefficient profiles in hyaline articular cartilage in order to reconstruct T(2) maps from the diffusion-weighted CPMG decays of apparent relaxation rates. Copyright © 2014 Elsevier Inc. All rights reserved.

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

PubMed Central

Farokh Payam, Amir; Piantanida, Luca; Cafolla, Clodomiro; Voïtchovsky, Kislon

2016-01-01

Atomic force microscopy (AFM) has become a well-established technique for nanoscale imaging of samples in air and in liquid. Recent studies have shown that when operated in amplitude-modulation (tapping) mode, atomic or molecular-level resolution images can be achieved over a wide range of soft and hard samples in liquid. In these situations, small oscillation amplitudes (SAM-AFM) enhance the resolution by exploiting the solvated liquid at the surface of the sample. Although the technique has been successfully applied across fields as diverse as materials science, biology and biophysics and surface chemistry, obtaining high-resolution images in liquid can still remain challenging for novice users. This is partly due to the large number of variables to control and optimize such as the choice of cantilever, the sample preparation, and the correct manipulation of the imaging parameters. Here, we present a protocol for achieving high-resolution images of hard and soft samples in fluid using SAM-AFM on a commercial instrument. Our goal is to provide a step-by-step practical guide to achieving high-resolution images, including the cleaning and preparation of the apparatus and the sample, the choice of cantilever and optimization of the imaging parameters. For each step, we explain the scientific rationale behind our choices to facilitate the adaptation of the methodology to every user's specific system. PMID:28060262

SU-C-207A-02: Proton Radiography Using Pencil Beam Scanning and a Novel, Low-Cost Range Telescope

DOE Office of Scientific and Technical Information (OSTI.GOV)

Dolney, D; Mayers, G; Newcomer, M

Purpose: While the energy of therapeutic proton beams can be adjusted to penetrate to any given depth in water, range uncertainties arise in patients due in part to imprecise knowledge of the stopping power of protons in human tissues [1]. Proton radiography is one approach to reduce the beam range uncertainty [2], thereby allowing for a reduction in treatment margins and dose escalation. Methods: The authors have adapted a novel detector technology based on Micromesh Gaseous Structure (“Micromegas”) for proton therapy beams and have demonstrated fine spatial and time resolution of magnetically scanned proton pencil beams, as well as widemore » dynamic range for dosimetry [3]. The authors have constructed a prototype imaging system comprised of 5 Micromegas layers. Proton radiographs were obtained downstream of solid water assemblies. The position-sensitive monitor chambers in the IBA proton delivery nozzle provide the beam entrance position. Results: Our technique achieves spatial resolution as low as 300 µm and water-equivalent thickness (WET) resolution as good as 0.02% (60 µm out of 31 cm total thickness). The dose delivered to the patient is kept below 2 cGy. The spatial resolution as a function of sample rate and number of delivered protons is found to be near the theoretical Cramer-Rao lower bound. By extrapolating the CR bound, we argue that the imaging dose could be further lowered to 1 mGy, while still achieving submillimeter spatial resolution, by achievable instrumentation and beam delivery modifications. Conclusion: For proton radiography, high spatial and WET resolution can be achieved, with minimal additional dose to patient, by using magnetically scanned proton pencil beams and Micromegas detectors.« less

Tests of Scintillator+WLS Strips for Muon System at Future Colliders

DOE Office of Scientific and Technical Information (OSTI.GOV)

Denisov, Dmitri; Evdokimov, Valery; Lukić, Strahinja

2015-10-11

Prototype scintilator+WLS strips with SiPM readout for muon system at future colliders were tested for light yield, time resolution and position resolution. Depending on the configuration, light yield of up to 36 photoelectrons per muon per SiPM has been achieved, as well as time resolution of 0.5 ns and position resolution of ~ 7 cm.

Joint denoising, demosaicing, and chromatic aberration correction for UHD video

NASA Astrophysics Data System (ADS)

Jovanov, Ljubomir; Philips, Wilfried; Damstra, Klaas Jan; Ellenbroek, Frank

2017-09-01

High-resolution video capture is crucial for numerous applications such as surveillance, security, industrial inspection, medical imaging and digital entertainment. In the last two decades, we are witnessing a dramatic increase of the spatial resolution and the maximal frame rate of video capturing devices. In order to achieve further resolution increase, numerous challenges will be facing us. Due to the reduced size of the pixel, the amount of light also reduces, leading to the increased noise level. Moreover, the reduced pixel size makes the lens imprecisions more pronounced, which especially applies to chromatic aberrations. Even in the case when high quality lenses are used some chromatic aberration artefacts will remain. Next, noise level additionally increases due to the higher frame rates. To reduce the complexity and the price of the camera, one sensor captures all three colors, by relying on Color Filter Arrays. In order to obtain full resolution color image, missing color components have to be interpolated, i.e. demosaicked, which is more challenging than in the case of lower resolution, due to the increased noise and aberrations. In this paper, we propose a new method, which jointly performs chromatic aberration correction, denoising and demosaicking. By jointly performing the reduction of all artefacts, we are reducing the overall complexity of the system and the introduction of new artefacts. In order to reduce possible flicker we also perform temporal video enhancement. We evaluate the proposed method on a number of publicly available UHD sequences and on sequences recorded in our studio.

Pseudo-differential CMOS analog front-end circuit for wide-bandwidth optical probe current sensor

NASA Astrophysics Data System (ADS)

Uekura, Takaharu; Oyanagi, Kousuke; Sonehara, Makoto; Sato, Toshiro; Miyaji, Kousuke

2018-04-01

In this paper, we present a pseudo-differential analog front-end (AFE) circuit for a novel optical probe current sensor (OPCS) aimed for high-frequency power electronics. It employs a regulated cascode transimpedance amplifier (RGC-TIA) to achieve a high gain and a large bandwidth without using an extremely high performance operational amplifier. The AFE circuit is designed in a 0.18 µm standard CMOS technology achieving a high transimpedance gain of 120 dB Ω and high cut off frequency of 16 MHz. The measured slew rate is 70 V/µs and the input referred current noise is 1.02 pA/\\sqrt{\\text{Hz}} . The magnetic resolution and bandwidth of OPCS are estimated to be 1.29 mTrms and 16 MHz, respectively; the bandwidth is higher than that of the reported Hall effect current sensor.

Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins.

PubMed

Kurdi, Said El; Muaileq, Dina Abu; Alhazmi, Hassan A; Bratty, Mohammed Al; Deeb, Sami El

2017-06-27

HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and second generation monolithic silica RP-18e columns and a fused core pentafluoro-phenyl propyl column were developed. Application of three fused core columns offered comparable separations of retinyl palmitate, DL-α-tocopheryl acetate, cholecalciferol and menadione in terms of elution speed and separation efficiency. Separation was achieved in approx. 5 min with good resolution (Rs > 5) and precision (RSD ≤ 0.6 %). Monolithic columns showed, however, a higher number of theoretical plates, better precision and lower column backpressure than the fused core column. The three developed methods were successfully applied to separate and quantitate fat-soluble vitamins in commercial products.

A flood map based DOI decoding method for block detector: a GATE simulation study.

PubMed

Shi, Han; Du, Dong; Su, Zhihong; Peng, Qiyu

2014-01-01

Positron Emission Tomography (PET) systems using detectors with Depth of Interaction (DOI) capabilities could achieve higher spatial resolution and better image quality than those without DOI. Up till now, most DOI methods developed are not cost-efficient for a whole body PET system. In this paper, we present a DOI decoding method based on flood map for low-cost conventional block detector with four-PMT readout. Using this method, the DOI information can be directly extracted from the DOI-related crystal spot deformation in the flood map. GATE simulations are then carried out to validate the method, confirming a DOI sorting accuracy of 85.27%. Therefore, we conclude that this method has the potential to be applied in conventional detectors to achieve a reasonable DOI measurement without dramatically increasing their complexity and cost of an entire PET system.

Chip-scale hermetic feedthroughs for implantable bionics.

PubMed

Guenther, Thomas; Dodds, Christopher W D; Lovell, Nigel H; Suaning, Gregg J

2011-01-01

Most implantable medical devices such as cochlear implants and visual prostheses require protection of the stimulating electronics. This is achieved by way of a hermetic feedthrough system which typically features three important attributes: biocompatibility with the human body, device hermeticity and density of feedthrough conductors. On the quest for building a visual neuroprosthesis, a high number of stimulating channels is required. This has encouraged new technologies with higher rates of production yield and further miniaturization. An Al(2)O(3) based feedthrough system has been developed comprising up to 20 platinum feedthroughs per square millimeter. Ceramics substrates are shown to have leak rates below 1 × 10(-12) atm × cc/s, thus exceeding the resolution limits of most commercially available leak detectors. A sheet resistance of 0.05 Ω can be achieved. This paper describes the design, fabrication process and hermeticity testing of high density feedthroughs for use in neuroprosthetic implants.

Study of the Vibrational Modes of Subsurface Oxygen on Al (111) Using Diode Laser Infrared Reflection-Absorption Spectroscopy.

DTIC Science & Technology

1987-10-15

apparent shift of this band to higher energy with increasing coverage, observed at lower resolution (but higher sensitivity) in electron energy loss...apparent shift of this band to higher energy with increasing coverage, observed at lower resolution (but higher sen- sitivity) in electron energy ...11 using high-resolution electron energy -loss spectroscopy (EELS), is especially intriguing. 02 dissociates on this surface to populate two types of

Implementation of parallel transmit beamforming using orthogonal frequency division multiplexing--achievable resolution and interbeam interference.

PubMed

Demi, Libertario; Viti, Jacopo; Kusters, Lieneke; Guidi, Francesco; Tortoli, Piero; Mischi, Massimo

2013-11-01

The speed of sound in the human body limits the achievable data acquisition rate of pulsed ultrasound scanners. To overcome this limitation, parallel beamforming techniques are used in ultrasound 2-D and 3-D imaging systems. Different parallel beamforming approaches have been proposed. They may be grouped into two major categories: parallel beamforming in reception and parallel beamforming in transmission. The first category is not optimal for harmonic imaging; the second category may be more easily applied to harmonic imaging. However, inter-beam interference represents an issue. To overcome these shortcomings and exploit the benefit of combining harmonic imaging and high data acquisition rate, a new approach has been recently presented which relies on orthogonal frequency division multiplexing (OFDM) to perform parallel beamforming in transmission. In this paper, parallel transmit beamforming using OFDM is implemented for the first time on an ultrasound scanner. An advanced open platform for ultrasound research is used to investigate the axial resolution and interbeam interference achievable with parallel transmit beamforming using OFDM. Both fundamental and second-harmonic imaging modalities have been considered. Results show that, for fundamental imaging, axial resolution in the order of 2 mm can be achieved in combination with interbeam interference in the order of -30 dB. For second-harmonic imaging, axial resolution in the order of 1 mm can be achieved in combination with interbeam interference in the order of -35 dB.

Power spectral estimation of high-harmonics in echoes of wall resonances to improve resolution in non-invasive measurements of wall mechanical properties in rubber tube and ex-vivo artery.

PubMed

Bazan, I; Ramos, A; Balay, G; Negreira, C

2018-07-01

The aim of this work is to develop a new type of ultrasonic analysis of the mechanical properties of an arterial wall with improved resolution, and to confirm its feasibility under laboratory conditions. it is expected that this would facilitate a non-invasive path for accurate predictive diagnosis that enables an early detection & therapy of vascular pathologies. In particular, the objective is to detect and quantify the small elasticity changes (in Young's modulus E) of arterial walls, which precede pathology. A submicron axial resolution is required for this analysis, as the periodic widening of the wall (under oscillatory arterial pressure) varies between ±10 and 20 μm. This high resolution represents less than 1% of the parietal thickness (e.g., < 7 μm in carotid arteries). The novelty of our proposal is the new technique used to estimate the modulus E of the arterial walls, which achieves the requisite resolution. It calculates the power spectral evolution associated with the temporal dynamics in higher harmonics of the wall internal resonance f 0 . This was attained via the implementation of an autoregressive parametric algorithm that accurately detects parietal echo-dynamics during a heartbeat. Thus, it was possible to measure the punctual elasticity of the wall, with a higher resolution (> an order of magnitude) compared to conventional approaches. The resolution of a typical ultrasonic image is limited to several hundred microns, and thus, such small changes are undetected. The proposed procedure provides a non-invasive and direct measure of elasticity by doing an estimation of changes in the Nf 0 harmonics and wall thickness with a resolution of 0.1%, for first time. The results obtained by using the classic temporal cross-correlation method (TCC) were compared to those obtained with the new procedure. The latter allowed the evaluation of alterations in the elastic properties of arterial walls that are 30 times smaller than those being detectable with TCC; in fact, the depth resolution of the TCC approach is limited to ≈20 μm for typical SNRs. These values were calculated based on echoes obtained using a reference pattern (rubber tube). The application of the proposed procedure was also confirmed via "ex-vivo" measurements in pig carotid segments. Copyright © 2018 Elsevier B.V. All rights reserved.

High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jun, Ji Hyun; Song, Zhihong; Liu, Zhenjiu

High-spatial resolution and high-mass resolution techniques are developed and adopted for the mass spectrometric imaging of epicuticular lipids on the surface of Arabidopsis thaliana. Single cell level spatial resolution of {approx}12 {micro}m was achieved by reducing the laser beam size by using an optical fiber with 25 {micro}m core diameter in a vacuum matrix-assisted laser desorption ionization-linear ion trap (vMALDI-LTQ) mass spectrometer and improved matrix application using an oscillating capillary nebulizer. Fine chemical images of a whole flower were visualized in this high spatial resolution showing substructure of an anther and single pollen grains at the stigma and anthers. Themore » LTQ-Orbitrap with a MALDI ion source was adopted to achieve MS imaging in high mass resolution. Specifically, isobaric silver ion adducts of C29 alkane (m/z 515.3741) and C28 aldehyde (m/z 515.3377), indistinguishable in low-resolution LTQ, can now be clearly distinguished and their chemical images could be separately constructed. In the application to roots, the high spatial resolution allowed molecular MS imaging of secondary roots and the high mass resolution allowed direct identification of lipid metabolites on root surfaces.« less

SU-E-J-88: Deformable Registration Using Multi-Resolution Demons Algorithm for 4DCT.

PubMed

Li, Dengwang; Yin, Yong

2012-06-01

In order to register 4DCT efficiently, we propose an improved deformable registration algorithm based on improved multi-resolution demons strategy to improve the efficiency of the algorithm. 4DCT images of lung cancer patients are collected from a General Electric Discovery ST CT scanner from our cancer hospital. All of the images are sorted into groups and reconstructed according to their phases, and eachrespiratory cycle is divided into 10 phases with the time interval of 10%. Firstly, in our improved demons algorithm we use gradients of both reference and floating images as deformation forces and also redistribute the forces according to the proportion of the two forces. Furthermore, we introduce intermediate variable to cost function for decreasing the noise in registration process. At the same time, Gaussian multi-resolution strategy and BFGS method for optimization are used to improve speed and accuracy of the registration. To validate the performance of the algorithm, we register the previous 10 phase-images. We compared the difference of floating and reference images before and after registered where two landmarks are decided by experienced clinician. We registered 10 phase-images of 4D-CT which is lung cancer patient from cancer hospital and choose images in exhalationas the reference images, and all other images were registered into the reference images. This method has a good accuracy demonstrated by a higher similarity measure for registration of 4D-CT and it can register a large deformation precisely. Finally, we obtain the tumor target achieved by the deformation fields using proposed method, which is more accurately than the internal margin (IM) expanded by the Gross Tumor Volume (GTV). Furthermore, we achieve tumor and normal tissue tracking and dose accumulation using 4DCT data. An efficient deformable registration algorithm was proposed by using multi-resolution demons algorithm for 4DCT. © 2012 American Association of Physicists in Medicine.

Pole Photogrammetry with AN Action Camera for Fast and Accurate Surface Mapping

NASA Astrophysics Data System (ADS)

Gonçalves, J. A.; Moutinho, O. F.; Rodrigues, A. C.

2016-06-01

High resolution and high accuracy terrain mapping can provide height change detection for studies of erosion, subsidence or land slip. A UAV flying at a low altitude above the ground, with a compact camera, acquires images with resolution appropriate for these change detections. However, there may be situations where different approaches may be needed, either because higher resolution is required or the operation of a drone is not possible. Pole photogrammetry, where a camera is mounted on a pole, pointing to the ground, is an alternative. This paper describes a very simple system of this kind, created for topographic change detection, based on an action camera. These cameras have high quality and very flexible image capture. Although radial distortion is normally high, it can be treated in an auto-calibration process. The system is composed by a light aluminium pole, 4 meters long, with a 12 megapixel GoPro camera. Average ground sampling distance at the image centre is 2.3 mm. The user moves along a path, taking successive photos, with a time lapse of 0.5 or 1 second, and adjusting the speed in order to have an appropriate overlap, with enough redundancy for 3D coordinate extraction. Marked ground control points are surveyed with GNSS for precise georeferencing of the DSM and orthoimage that are created by structure from motion processing software. An average vertical accuracy of 1 cm could be achieved, which is enough for many applications, for example for soil erosion. The GNSS survey in RTK mode with permanent stations is now very fast (5 seconds per point), which results, together with the image collection, in a very fast field work. If an improved accuracy is needed, since image resolution is 1/4 cm, it can be achieved using a total station for the control point survey, although the field work time increases.

Long-term efficacy of a tongue tie service in improving breast feeding rates: A prospective study.

PubMed

Billington, Jennifer; Yardley, Iain; Upadhyaya, Manasvi

2018-02-01

Breast feeding rates in England at 3months of age are approximately 17% for exclusive breast-feeding and 55% for breast-feeds supplemented with formula. Tongue-tie (TT) in infants is cited as a significant cause of difficulty with maintaining breast-feeding. Early treatment and support can improve breast-feeding and allow infants to benefit from the many long-term benefits of breast-feeding. Our aim was to determine BF rates in infants 3months after attending our tongue-tie clinic (TTC). Institutional ethical approval and study approval were obtained. Patients attending the TTC from May to July 2016 were included. Telephone contact was made 3months postprocedure to establish current behaviour. Symptom resolution was recorded as complete resolution (CR), moderate resolution (MoR), or minimal resolution (MiR). Feeding pattern was either exclusively BF or combined breast and formula feeds or exclusively formula fed. 100 infants were included with complete follow-up data on 87 (87% response rate). Median age at release of TT was 17 (2-88) days without any recurrence. Status at 3months was CR (n=70, 80%); MoR (n=13, 15%), and MiR (n=4, 5%). 43 (49%) were exclusively BF, 36 (41%) were supplementing BF with some formula (2/3 by choice and 1/3 owing to insufficient milk production), and 8 (10%) were using only formula milk (7 by choice and 2 owing to ongoing feeding difficulties). Of the 17 mothers still experiencing symptoms, 5 were exclusively breast-feeding, and 8 were persisting with combined feeds. Infants attending our tongue-tie clinic achieved both higher exclusive breastfeeding and combined breast and bottle-feeding when compared to national breast-feeding data at 3months of age. This can facilitate the achievement of long-term breastfeeding, exposing infants and mothers to many of the associated benefits. 4. Copyright © 2017 Elsevier Inc. All rights reserved.

The Session of a Special Character of UNEP's Governing Council: Principal Resolutions.

ERIC Educational Resources Information Center

Uniterra, 1982

1982-01-01

Presents principal resolutions adopted by the Session of a Special Character (SSC) of the Governing Council of the United Nations Environment Programme. Resolutions focus on achievements of Action Plan for Human Environment, new perceptions of environmental issues, environmental trends, and planning/implementation of environmental activities.…

Circuit modification in electrical field flow fractionation systems generating higher resolution separation of nanoparticles.

PubMed

Tasci, Tonguc O; Johnson, William P; Fernandez, Diego P; Manangon, Eliana; Gale, Bruce K

2014-10-24

Compared to other sub-techniques of field flow fractionation (FFF), cyclical electrical field flow fractionation (CyElFFF) is a relatively new method with many opportunities remaining for improvement. One of the most important limitations of this method is the separation of particles smaller than 100nm. For such small particles, the diffusion rate becomes very high, resulting in severe reductions in the CyElFFF separation efficiency. To address this limitation, we modified the electrical circuitry of the ElFFF system. In all earlier ElFFF reports, electrical power sources have been directly connected to the ElFFF channel electrodes, and no alteration has been made in the electrical circuitry of the system. In this work, by using discrete electrical components, such as resistors and diodes, we improved the effective electric field in the system to allow high resolution separations. By modifying the electrical circuitry of the ElFFF system, high resolution separations of 15 and 40nm gold nanoparticles were achieved. The effects of applying different frequencies, amplitudes and voltage shapes have been investigated and analyzed through experiments. Copyright © 2014 Elsevier B.V. All rights reserved.

High-performance software-only H.261 video compression on PC

NASA Astrophysics Data System (ADS)

Kasperovich, Leonid

1996-03-01

This paper describes an implementation of a software H.261 codec for PC, that takes an advantage of the fast computational algorithms for DCT-based video compression, which have been presented by the author at the February's 1995 SPIE/IS&T meeting. The motivation for developing the H.261 prototype system is to demonstrate a feasibility of real time software- only videoconferencing solution to operate across a wide range of network bandwidth, frame rate, and resolution of the input video. As the bandwidths of current network technology will be increased, the higher frame rate and resolution of video to be transmitted is allowed, that requires, in turn, a software codec to be able to compress pictures of CIF (352 X 288) resolution at up to 30 frame/sec. Running on Pentium 133 MHz PC the codec presented is capable to compress video in CIF format at 21 - 23 frame/sec. This result is comparable to the known hardware-based H.261 solutions, but it doesn't require any specific hardware. The methods to achieve high performance, the program optimization technique for Pentium microprocessor along with the performance profile, showing the actual contribution of the different encoding/decoding stages to the overall computational process, are presented.

Micromachined PIN-PMN-PT Crystal Composite Transducer for High-Frequency Intravascular Ultrasound (IVUS) Imaging

PubMed Central

Li, Xiang; Ma, Teng; Tian, Jian; Han, Pengdi; Zhou, Qifa; Shung, K. Kirk

2015-01-01

In this paper, we report the use of micromachined PbIn1/2Nb1/2O3–PbMg1/3Nb2/3O3–PbTiO3 (PIN-PMN-PT) single crystal 1–3 composite material for intravascular ultrasound (IVUS) imaging application. The effective electromechanical coupling coefficient kt(eff) of the composite was measured to be 0.75 to 0.78. Acoustic impedance was estimated to be 20 MRayl. Based on the composite, needle-type and flexible-type IVUS transducers were fabricated. The composite transducer achieved an 86% bandwidth at the center frequency of 41 MHz, which resulted in a 43 μm axial resolution. Ex vivo IVUS imaging was conducted to demonstrate the improvement of axial resolution. The composite transducer was capable of identifying the three layers of a cadaver coronary artery specimen with high resolution. The PIN-PMN-PT-based composite has superior piezoelectric properties comparable to PMN-PT-based composite and its thermal stability is higher than PMN-PT. PIN-PMN-PT crystal can be an alternative approach for fabricating high-frequency composite, instead of using PMN-PT. PMID:24960706

Finite element analyses of thin film active grazing incidence x-ray optics

NASA Astrophysics Data System (ADS)

Davis, William N.; Reid, Paul B.; Schwartz, Daniel A.

2010-09-01

The Chandra X-ray Observatory, with its sub-arc second resolution, has revolutionized X-ray astronomy by revealing an extremely complex X-ray sky and demonstrating the power of the X-ray window in exploring fundamental astrophysical problems. Larger area telescopes of still higher angular resolution promise further advances. We are engaged in the development of a mission concept, Generation-X, a 0.1 arc second resolution x-ray telescope with tens of square meters of collecting area, 500 times that of Chandra. To achieve these two requirements of imaging and area, we are developing a grazing incidence telescope comprised of many mirror segments. Each segment is an adjustable mirror that is a section of a paraboloid or hyperboloid, aligned and figure corrected in situ on-orbit. To that end, finite element analyses of thin glass mirrors are performed to determine influence functions for each actuator on the mirrors, in order to develop algorithms for correction of mirror deformations. The effects of several mirror mounting schemes are also studied. The finite element analysis results, combined with measurements made on prototype mirrors, will be used to further refine the correction algorithms.

FPGA-Based Front-End Electronics for Positron Emission Tomography

PubMed Central

Haselman, Michael; DeWitt, Don; McDougald, Wendy; Lewellen, Thomas K.; Miyaoka, Robert; Hauck, Scott

2010-01-01

Modern Field Programmable Gate Arrays (FPGAs) are capable of performing complex discrete signal processing algorithms with clock rates above 100MHz. This combined with FPGA’s low expense, ease of use, and selected dedicated hardware make them an ideal technology for a data acquisition system for positron emission tomography (PET) scanners. Our laboratory is producing a high-resolution, small-animal PET scanner that utilizes FPGAs as the core of the front-end electronics. For this next generation scanner, functions that are typically performed in dedicated circuits, or offline, are being migrated to the FPGA. This will not only simplify the electronics, but the features of modern FPGAs can be utilizes to add significant signal processing power to produce higher resolution images. In this paper two such processes, sub-clock rate pulse timing and event localization, will be discussed in detail. We show that timing performed in the FPGA can achieve a resolution that is suitable for small-animal scanners, and will outperform the analog version given a low enough sampling period for the ADC. We will also show that the position of events in the scanner can be determined in real time using a statistical positioning based algorithm. PMID:21961085

Imaging nanoscale spatial modulation of a relativistic electron beam with a MeV ultrafast electron microscope

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lu, Chao; Jiang, Tao; Liu, Shengguang

Here, an accelerator-based MeV ultrafast electron microscope (MUEM) has been proposed as a promising tool to the study structural dynamics at the nanometer spatial scale and the picosecond temporal scale. Here, we report experimental tests of a prototype MUEM where high quality images with nanoscale fine structures were recorded with a pulsed ~3 MeV picosecond electron beam. The temporal and spatial resolutions of the MUEM operating in the single-shot mode are about 4 ps (FWHM) and 100 nm (FWHM), corresponding to a temporal-spatial resolution of 4 × 10 –19 sm, about 2 orders of magnitude higher than that achieved withmore » state-of-the-art single-shot keV UEM. Using this instrument, we offer the demonstration of visualizing the nanoscale periodic spatial modulation of an electron beam, which may be converted into longitudinal density modulation through emittance exchange to enable production of high-power coherent radiation at short wavelengths. Our results mark a great step towards single-shot nanometer-resolution MUEMs and compact intense x-ray sources that may have widespread applications in many areas of science.« less

Characterization of applied fields for ion mobility separations in traveling wave based structures for lossless ion manipulations (SLIM)

DOE PAGES

Hamid, Ahmed M.; Prabhakaran, Aneesh; Garimella, Sandilya V. B.; ...

2018-03-26

Ion mobility (IM) is rapidly gaining attention for the separation and analysis of biomolecules due to the ability to distinguish the shapes of ions. However, conventional constant electric field drift tube IM separations have limited resolving power, constrained by practical limitations on the path length and maximum applied voltage. The implementation of traveling waves (TW) in IM removes the latter limitation, allowing higher resolution to be achieved using extended path lengths. Both of these can be readily obtained in Structures for Lossless Ion Manipulations (SLIM), which are fabricated from arrays of electrodes patterned on two parallel surfaces where potentials aremore » applied to generate appropriate electric fields between the surfaces. Here we have investigated the relationship between the primary SLIM variables, such as electrode dimensions, inter-surface gap, and the applied TW voltages, that directly impact the fields experienced by ions. Ion trajectory simulations and theoretical calculations have been utilized to understand the dependence of SLIM geometry and effective electric fields on IM resolution. The variables explored impact both ion confinement and the observed IM resolution using SLIM modules.« less

Precise Point Positioning with Partial Ambiguity Fixing.

PubMed

Li, Pan; Zhang, Xiaohong

2015-06-10

Reliable and rapid ambiguity resolution (AR) is the key to fast precise point positioning (PPP). We propose a modified partial ambiguity resolution (PAR) method, in which an elevation and standard deviation criterion are first used to remove the low-precision ambiguity estimates for AR. Subsequently the success rate and ratio-test are simultaneously used in an iterative process to increase the possibility of finding a subset of decorrelated ambiguities which can be fixed with high confidence. One can apply the proposed PAR method to try to achieve an ambiguity-fixed solution when full ambiguity resolution (FAR) fails. We validate this method using data from 450 stations during DOY 021 to 027, 2012. Results demonstrate the proposed PAR method can significantly shorten the time to first fix (TTFF) and increase the fixing rate. Compared with FAR, the average TTFF for PAR is reduced by 14.9% for static PPP and 15.1% for kinematic PPP. Besides, using the PAR method, the average fixing rate can be increased from 83.5% to 98.2% for static PPP, from 80.1% to 95.2% for kinematic PPP respectively. Kinematic PPP accuracy with PAR can also be significantly improved, compared to that with FAR, due to a higher fixing rate.

Precise Point Positioning with Partial Ambiguity Fixing

PubMed Central

Li, Pan; Zhang, Xiaohong

2015-01-01

Reliable and rapid ambiguity resolution (AR) is the key to fast precise point positioning (PPP). We propose a modified partial ambiguity resolution (PAR) method, in which an elevation and standard deviation criterion are first used to remove the low-precision ambiguity estimates for AR. Subsequently the success rate and ratio-test are simultaneously used in an iterative process to increase the possibility of finding a subset of decorrelated ambiguities which can be fixed with high confidence. One can apply the proposed PAR method to try to achieve an ambiguity-fixed solution when full ambiguity resolution (FAR) fails. We validate this method using data from 450 stations during DOY 021 to 027, 2012. Results demonstrate the proposed PAR method can significantly shorten the time to first fix (TTFF) and increase the fixing rate. Compared with FAR, the average TTFF for PAR is reduced by 14.9% for static PPP and 15.1% for kinematic PPP. Besides, using the PAR method, the average fixing rate can be increased from 83.5% to 98.2% for static PPP, from 80.1% to 95.2% for kinematic PPP respectively. Kinematic PPP accuracy with PAR can also be significantly improved, compared to that with FAR, due to a higher fixing rate. PMID:26067196

Imaging nanoscale spatial modulation of a relativistic electron beam with a MeV ultrafast electron microscope

DOE PAGES

Lu, Chao; Jiang, Tao; Liu, Shengguang; ...

2018-03-12

Here, an accelerator-based MeV ultrafast electron microscope (MUEM) has been proposed as a promising tool to the study structural dynamics at the nanometer spatial scale and the picosecond temporal scale. Here, we report experimental tests of a prototype MUEM where high quality images with nanoscale fine structures were recorded with a pulsed ~3 MeV picosecond electron beam. The temporal and spatial resolutions of the MUEM operating in the single-shot mode are about 4 ps (FWHM) and 100 nm (FWHM), corresponding to a temporal-spatial resolution of 4 × 10 –19 sm, about 2 orders of magnitude higher than that achieved withmore » state-of-the-art single-shot keV UEM. Using this instrument, we offer the demonstration of visualizing the nanoscale periodic spatial modulation of an electron beam, which may be converted into longitudinal density modulation through emittance exchange to enable production of high-power coherent radiation at short wavelengths. Our results mark a great step towards single-shot nanometer-resolution MUEMs and compact intense x-ray sources that may have widespread applications in many areas of science.« less

Characterization of applied fields for ion mobility separations in traveling wave based structures for lossless ion manipulations (SLIM)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hamid, Ahmed M.; Prabhakaran, Aneesh; Garimella, Sandilya V. B.

Ion mobility (IM) is rapidly gaining attention for the separation and analysis of biomolecules due to the ability to distinguish the shapes of ions. However, conventional constant electric field drift tube IM separations have limited resolving power, constrained by practical limitations on the path length and maximum applied voltage. The implementation of traveling waves (TW) in IM removes the latter limitation, allowing higher resolution to be achieved using extended path lengths. Both of these can be readily obtained in Structures for Lossless Ion Manipulations (SLIM), which are fabricated from arrays of electrodes patterned on two parallel surfaces where potentials aremore » applied to generate appropriate electric fields between the surfaces. Here we have investigated the relationship between the primary SLIM variables, such as electrode dimensions, inter-surface gap, and the applied TW voltages, that directly impact the fields experienced by ions. Ion trajectory simulations and theoretical calculations have been utilized to understand the dependence of SLIM geometry and effective electric fields on IM resolution. The variables explored impact both ion confinement and the observed IM resolution using SLIM modules.« less

Hybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical Magnifications.

PubMed

Zhang, Yu Shrike; Chang, Jae-Byum; Alvarez, Mario Moisés; Trujillo-de Santiago, Grissel; Aleman, Julio; Batzaya, Byambaa; Krishnadoss, Vaishali; Ramanujam, Aishwarya Aravamudhan; Kazemzadeh-Narbat, Mehdi; Chen, Fei; Tillberg, Paul W; Dokmeci, Mehmet Remzi; Boyden, Edward S; Khademhosseini, Ali

2016-03-15

To date, much effort has been expended on making high-performance microscopes through better instrumentation. Recently, it was discovered that physical magnification of specimens was possible, through a technique called expansion microscopy (ExM), raising the question of whether physical magnification, coupled to inexpensive optics, could together match the performance of high-end optical equipment, at a tiny fraction of the price. Here we show that such "hybrid microscopy" methods--combining physical and optical magnifications--can indeed achieve high performance at low cost. By physically magnifying objects, then imaging them on cheap miniature fluorescence microscopes ("mini-microscopes"), it is possible to image at a resolution comparable to that previously attainable only with benchtop microscopes that present costs orders of magnitude higher. We believe that this unprecedented hybrid technology that combines expansion microscopy, based on physical magnification, and mini-microscopy, relying on conventional optics--a process we refer to as Expansion Mini-Microscopy (ExMM)--is a highly promising alternative method for performing cost-effective, high-resolution imaging of biological samples. With further advancement of the technology, we believe that ExMM will find widespread applications for high-resolution imaging particularly in research and healthcare scenarios in undeveloped countries or remote places.

Probing evolutionary population synthesis models in the near infrared with early-type galaxies

NASA Astrophysics Data System (ADS)

Dahmer-Hahn, Luis Gabriel; Riffel, Rogério; Rodríguez-Ardila, Alberto; Martins, Lucimara P.; Kehrig, Carolina; Heckman, Timothy M.; Pastoriza, Miriani G.; Dametto, Natacha Z.

2018-06-01

We performed a near-infrared (NIR; ˜1.0 -2.4 μm) stellar population study in a sample of early-type galaxies. The synthesis was performed using five different evolutionary population synthesis libraries of models. Our main results can be summarized as follows: low-spectral-resolution libraries are not able to produce reliable results when applied to the NIR alone, with each library finding a different dominant population. The two newest higher resolution models, on the other hand, perform considerably better, finding consistent results to each other and to literature values. We also found that optical results are consistent with each other even for lower resolution models. We also compared optical and NIR results and found out that lower resolution models tend to disagree in the optical and in the NIR, with higher fraction of young populations in the NIR and dust extinction ˜1 mag higher than optical values. For higher resolution models, optical and NIR results tend to agree much better, suggesting that a higher spectral resolution is fundamental to improve the quality of the results.

Fast time-resolved electrostatic force microscopy: Achieving sub-cycle time resolution

DOE Office of Scientific and Technical Information (OSTI.GOV)

Karatay, Durmus U.; Harrison, Jeffrey S.; Glaz, Micah S.

The ability to measure microsecond- and nanosecond-scale local dynamics below the diffraction limit with widely available atomic force microscopy hardware would enable new scientific studies in fields ranging from biology to semiconductor physics. However, commercially available scanning-probe instruments typically offer the ability to measure dynamics only on time scales of milliseconds to seconds. Here, we describe in detail the implementation of fast time-resolved electrostatic force microscopy using an oscillating cantilever as a means to measure fast local dynamics following a perturbation to a sample. We show how the phase of the oscillating cantilever relative to the perturbation event is criticalmore » to achieving reliable sub-cycle time resolution. We explore how noise affects the achievable time resolution and present empirical guidelines for reducing noise and optimizing experimental parameters. Specifically, we show that reducing the noise on the cantilever by using photothermal excitation instead of piezoacoustic excitation further improves time resolution. We demonstrate the discrimination of signal rise times with time constants as fast as 10 ns, and simultaneous data acquisition and analysis for dramatically improved image acquisition times.« less

Achieving the resolution of the spectrograph of the 6m large Azimuthal telescope

NASA Astrophysics Data System (ADS)

Sazonenko, Dmitrii; Kukushkin, Dmitrii; Bakholdin, Alexey; Valyavin, Gennady

2016-08-01

Special Astrophysical Observatory of Russian Academy of Sciences (SAO RAS) creates a spectrograph with high spectral resolution for the 6-meter telescope. The spectrograph consists of a mobile unit located at the focus of the telescope's main mirror, a stationary part located under the telescope and optical fibers which transmit light from the mobile part to the stationary one. The spectral resolution of the stationary part should be R=100000. To achieve such a value, the scheme has two spectral elements, with cross-dispersion. The main spectral element is an echelle grating. The second spectral element is a prism with a diffraction grating on one facet.

Very high resolution aerial films

NASA Astrophysics Data System (ADS)

Becker, Rolf

1986-11-01

The use of very high resolution aerial films in aerial photography is evaluated. Commonly used panchromatic, color, and CIR films and their high resolution equivalents are compared. Based on practical experience and systematic investigations, the very high image quality and improved height accuracy that can be achieved using these films are demonstrated. Advantages to be gained from this improvement and operational restrictions encountered when using high resolution film are discussed.

Evaluation of Large Volume SrI2(Eu) Scintillator Detectors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sturm, B W; Cherepy, N J; Drury, O B

2010-11-18

There is an ever increasing demand for gamma-ray detectors which can achieve good energy resolution, high detection efficiency, and room-temperature operation. We are working to address each of these requirements through the development of large volume SrI{sub 2}(Eu) scintillator detectors. In this work, we have evaluated a variety of SrI{sub 2} crystals with volumes >10 cm{sup 3}. The goal of this research was to examine the causes of energy resolution degradation for larger detectors and to determine what can be done to mitigate these effects. Testing both packaged and unpackaged detectors, we have consistently achieved better resolution with the packagedmore » detectors. Using a collimated gamma-ray source, it was determined that better energy resolution for the packaged detectors is correlated with better light collection uniformity. A number of packaged detectors were fabricated and tested and the best spectroscopic performance was achieved for a 3% Eu doped crystal with an energy resolution of 2.93% FWHM at 662keV. Simulations of SrI{sub 2}(Eu) crystals were also performed to better understand the light transport physics in scintillators and are reported. This study has important implications for the development of SrI{sub 2}(Eu) detectors for national security purposes.« less

Propagation phasor approach for holographic image reconstruction

PubMed Central

Luo, Wei; Zhang, Yibo; Göröcs, Zoltán; Feizi, Alborz; Ozcan, Aydogan

2016-01-01

To achieve high-resolution and wide field-of-view, digital holographic imaging techniques need to tackle two major challenges: phase recovery and spatial undersampling. Previously, these challenges were separately addressed using phase retrieval and pixel super-resolution algorithms, which utilize the diversity of different imaging parameters. Although existing holographic imaging methods can achieve large space-bandwidth-products by performing pixel super-resolution and phase retrieval sequentially, they require large amounts of data, which might be a limitation in high-speed or cost-effective imaging applications. Here we report a propagation phasor approach, which for the first time combines phase retrieval and pixel super-resolution into a unified mathematical framework and enables the synthesis of new holographic image reconstruction methods with significantly improved data efficiency. In this approach, twin image and spatial aliasing signals, along with other digital artifacts, are interpreted as noise terms that are modulated by phasors that analytically depend on the lateral displacement between hologram and sensor planes, sample-to-sensor distance, wavelength, and the illumination angle. Compared to previous holographic reconstruction techniques, this new framework results in five- to seven-fold reduced number of raw measurements, while still achieving a competitive resolution and space-bandwidth-product. We also demonstrated the success of this approach by imaging biological specimens including Papanicolaou and blood smears. PMID:26964671

Portable, Battery Operated Capillary Electrophoresis with Optical Isomer Resolution Integrated with Ionization Source for Mass Spectrometry

NASA Astrophysics Data System (ADS)

Moini, Mehdi; Rollman, Christopher M.

2016-03-01

We introduce a battery operated capillary electrophoresis electrospray ionization (CE/ESI) source for mass spectrometry with optical isomer separation capability. The source fits in front of low or high resolution mass spectrometers similar to a nanospray source with about the same weight and size. The source has two high voltage power supplies (±25 kV HVPS) capable of operating in forward or reverse polarity modes and powered by a 12 V rechargeable lithium ion battery with operation time of ~10 h. In ultrafast CE mode, in which short narrow capillaries (≤15 μm i.d., 15-25 cm long) and field gradients ≥1000 V/cm are used, peak widths at the base are <1 s wide. Under these conditions, the source provides high resolution separation, including optical isomer resolution in ~1 min. Using a low resolution mass spectrometer (LTQ Velos) with a scan time of 0.07 s/scan, baseline separation of amino acids and their optical isomers were achieved in ~1 min. Moreover, bovine serum albumin (BSA) was analyzed in ~1 min with 56% coverage using the data-dependent MS/MS. Using a high resolution mass spectrometer (Thermo Orbitrap Elite) with 15,000 resolution, the fastest scan time achieved was 0.15 s, which was adequate for CE-MS analysis when optical isomer separation is not required or when the optical isomers were well separated. Figures of merit including a detection limit of 2 fmol and linear dynamic range of two orders of magnitude were achieved for amino acids.

Towards breaking the spatial resolution barriers: An optical flow and super-resolution approach for sea ice motion estimation

NASA Astrophysics Data System (ADS)

Petrou, Zisis I.; Xian, Yang; Tian, YingLi

2018-04-01

Estimation of sea ice motion at fine scales is important for a number of regional and local level applications, including modeling of sea ice distribution, ocean-atmosphere and climate dynamics, as well as safe navigation and sea operations. In this study, we propose an optical flow and super-resolution approach to accurately estimate motion from remote sensing images at a higher spatial resolution than the original data. First, an external example learning-based super-resolution method is applied on the original images to generate higher resolution versions. Then, an optical flow approach is applied on the higher resolution images, identifying sparse correspondences and interpolating them to extract a dense motion vector field with continuous values and subpixel accuracies. Our proposed approach is successfully evaluated on passive microwave, optical, and Synthetic Aperture Radar data, proving appropriate for multi-sensor applications and different spatial resolutions. The approach estimates motion with similar or higher accuracy than the original data, while increasing the spatial resolution of up to eight times. In addition, the adopted optical flow component outperforms a state-of-the-art pattern matching method. Overall, the proposed approach results in accurate motion vectors with unprecedented spatial resolutions of up to 1.5 km for passive microwave data covering the entire Arctic and 20 m for radar data, and proves promising for numerous scientific and operational applications.

From structure to structural dynamics: Ahmed Zewail's legacy.

PubMed

Chergui, Majed; Thomas, John Meurig

2017-07-01

In this brief tribute to Ahmed Zewail, we highlight and place in the historical context, several of the major achievements that he and his colleagues have made in Femtochemistry (of which he was the principal instigator) and his introduction of ultrafast electron scattering, diffraction, microscopy and spectroscopy. By achieving a sub-picosecond temporal resolution, coupled with a picometer spatial resolution, he revolutionised our understanding of the corpus of chemical, physical, biological and materials science systems.

Detecting reciprocity at a global scale

PubMed Central

Frank, Morgan R.; Obradovich, Nick; Sun, Lijun; Woon, Wei Lee; LeVeck, Brad L.; Rahwan, Iyad

2018-01-01

Reciprocity stabilizes cooperation from the level of microbes all the way up to humans interacting in small groups, but does reciprocity also underlie stable cooperation between larger human agglomerations, such as nation states? Famously, evolutionary models show that reciprocity could emerge as a widespread strategy for achieving international cooperation. However, existing studies have only detected reciprocity-driven cooperation in a small number of country pairs. We apply a new method for detecting mutual influence in dynamical systems to a new large-scale data set that records state interactions with high temporal resolution. Doing so, we detect reciprocity between many country pairs in the international system and find that these reciprocating country pairs exhibit qualitatively different cooperative dynamics when compared to nonreciprocating pairs. Consistent with evolutionary theories of cooperation, reciprocating country pairs exhibit higher levels of stable cooperation and are more likely to punish instances of noncooperation. However, countries in reciprocity-based relationships are also quicker to forgive single acts of noncooperation by eventually returning to previous levels of mutual cooperation. By contrast, nonreciprocating pairs are more likely to exploit each other’s cooperation via higher rates of defection. Together, these findings provide the strongest evidence to date that reciprocity is a widespread mechanism for achieving international cooperation. PMID:29326983

Detecting reciprocity at a global scale.

PubMed

Frank, Morgan R; Obradovich, Nick; Sun, Lijun; Woon, Wei Lee; LeVeck, Brad L; Rahwan, Iyad

2018-01-01

Reciprocity stabilizes cooperation from the level of microbes all the way up to humans interacting in small groups, but does reciprocity also underlie stable cooperation between larger human agglomerations, such as nation states? Famously, evolutionary models show that reciprocity could emerge as a widespread strategy for achieving international cooperation. However, existing studies have only detected reciprocity-driven cooperation in a small number of country pairs. We apply a new method for detecting mutual influence in dynamical systems to a new large-scale data set that records state interactions with high temporal resolution. Doing so, we detect reciprocity between many country pairs in the international system and find that these reciprocating country pairs exhibit qualitatively different cooperative dynamics when compared to nonreciprocating pairs. Consistent with evolutionary theories of cooperation, reciprocating country pairs exhibit higher levels of stable cooperation and are more likely to punish instances of noncooperation. However, countries in reciprocity-based relationships are also quicker to forgive single acts of noncooperation by eventually returning to previous levels of mutual cooperation. By contrast, nonreciprocating pairs are more likely to exploit each other's cooperation via higher rates of defection. Together, these findings provide the strongest evidence to date that reciprocity is a widespread mechanism for achieving international cooperation.

Latest improvements on long wave p on n HgCdTe technology at Sofradir

NASA Astrophysics Data System (ADS)

Rubaldo, Laurent; Taalat, Rachid; Berthoz, Jocelyn; Maillard, Magalie; Péré-Laperne, Nicolas; Brunner, Alexandre; Guinedor, Pierre; Dargent, L.; Manissadjian, A.; Reibel, Y.; Kerlain, A.

2017-02-01

SOFRADIR is the worldwide leader on the cooled IR detector market for high-performance space, military and security applications thanks to a well mastered Mercury Cadmium Telluride (MCT) technology, and recently thanks to the acquisition of III-V technology: InSb, InGaAs, and QWIP quantum detectors. As a result, strong and continuous development efforts are deployed to deliver cutting edge products with improved performances in terms of spatial and thermal resolution, dark current, quantum efficiency, low excess noise and high operability. The actual trend in quantum IR detector development is the design of very small pixel, with the higher achievable operating temperature whatever the spectral band. Moreover maintaining the detector operability and image quality at higher temperature moreover for long wavelength is a major issue. This paper presents the recent developments achieved at Sofradir to meet this challenge for LW band MCT extrinsic p on n technology with a cut-off wavelength of 9.3μm at 90K. State of the art performances will be presented in terms of dark current, operability and NETD temperature dependency, quantum efficiency, MTF, and RFPN (Residual Fixed Pattern Noise) stability up to 100K.

Simultaneous Multi-Slice fMRI using Spiral Trajectories

PubMed Central

Zahneisen, Benjamin; Poser, Benedikt A.; Ernst, Thomas; Stenger, V. Andrew

2014-01-01

Parallel imaging methods using multi-coil receiver arrays have been shown to be effective for increasing MRI acquisition speed. However parallel imaging methods for fMRI with 2D sequences show only limited improvements in temporal resolution because of the long echo times needed for BOLD contrast. Recently, Simultaneous Multi-Slice (SMS) imaging techniques have been shown to increase fMRI temporal resolution by factors of four and higher. In SMS fMRI multiple slices can be acquired simultaneously using Echo Planar Imaging (EPI) and the overlapping slices are un-aliased using a parallel imaging reconstruction with multiple receivers. The slice separation can be further improved using the “blipped-CAIPI” EPI sequence that provides a more efficient sampling of the SMS 3D k-space. In this paper a blipped-spiral SMS sequence for ultra-fast fMRI is presented. The blipped-spiral sequence combines the sampling efficiency of spiral trajectories with the SMS encoding concept used in blipped-CAIPI EPI. We show that blipped spiral acquisition can achieve almost whole brain coverage at 3 mm isotropic resolution in 168 ms. It is also demonstrated that the high temporal resolution allows for dynamic BOLD lag time measurement using visual/motor and retinotopic mapping paradigms. The local BOLD lag time within the visual cortex following the retinotopic mapping stimulation of expanding flickering rings is directly measured and easily translated into an eccentricity map of the cortex. PMID:24518259

Abdominal applications of 3.0-T MR imaging: comparative review versus a 1.5-T system.

PubMed

Choi, Jin-Young; Kim, Myeong-Jin; Chung, Yong Eun; Kim, Ji Youn; Jones, Alun C; de Becker, Jan; van Cauteren, Marc

2008-01-01

With the development of dedicated receiver coils and increased gradient performance, 3.0-T magnetic resonance (MR) systems are gaining wider acceptance in clinical practice. The expected twofold increase in signal-to-noise ratio (SNR) compared with that of 1.5-T MR systems may help improve spatial resolution or increase temporal resolution when used with parallel acquisition techniques. Several issues must be considered when applying 3.0-T MR in the abdomen, including the alteration of the radiofrequency field and relaxation time, increase in energy deposition and susceptibility effects, and problems associated with motion artifacts. For the evaluation of liver lesions, higher SNR and greater resolution achieved with the 3.0-T system could translate into better detection of malignant lesions on T2-weighted images obtained with adjusted imaging parameters. For the evaluation of pancreatic and biliary diseases, high-resolution T2-weighted imaging using single-shot turbo spin-echo sequences is useful; improvement in SNR was noticeable on two-dimensional MR cholangiopancreatographic images. For the preoperative imaging of rectal cancer, a single-shot sequence is useful for dramatically decreasing imaging time while maintaining image quality. Substantial modification of examination protocols, with optimized imaging parameters and sequence designs along with ongoing development of hardware, could contribute to an increased role of the 3.0-T system for abdominal MR examinations.

Toward acquiring comprehensive radiosurgery field commissioning data using the PRESAGE®/ optical-CT 3D dosimetry system

NASA Astrophysics Data System (ADS)

Clift, Corey; Thomas, Andrew; Adamovics, John; Chang, Zheng; Das, Indra; Oldham, Mark

2010-03-01

Achieving accurate small field dosimetry is challenging. This study investigates the utility of a radiochromic plastic PRESAGE® read with optical-CT for the acquisition of radiosurgery field commissioning data from a Novalis Tx system with a high-definition multileaf collimator (HDMLC). Total scatter factors (Sc, p), beam profiles, and penumbrae were measured for five different radiosurgery fields (5, 10, 20, 30 and 40 mm) using a commercially available optical-CT scanner (OCTOPUS, MGS Research). The percent depth dose (PDD), beam profile and penumbra of the 10 mm field were also measured using a higher resolution in-house prototype CCD-based scanner. Gafchromic EBT® film was used for independent verification. Measurements of Sc, p made with PRESAGE® and film agreed with mini-ion chamber commissioning data to within 4% for every field (range 0.2-3.6% for PRESAGE®, and 1.6-3.6% for EBT). PDD, beam profile and penumbra measurements made with the two PRESAGE®/optical-CT systems and film showed good agreement with the high-resolution diode commissioning measurements with a competitive resolution (0.5 mm pixels). The in-house prototype optical-CT scanner allowed much finer resolution compared with previous applications of PRESAGE®. The advantages of the PRESAGE® system for small field dosimetry include 3D measurements, negligible volume averaging, directional insensitivity, an absence of beam perturbations, energy and dose rate independence.

Force Spectroscopy with 9-μs Resolution and Sub-pN Stability by Tailoring AFM Cantilever Geometry.

PubMed

Edwards, Devin T; Faulk, Jaevyn K; LeBlanc, Marc-André; Perkins, Thomas T

2017-12-19

Atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS) is a powerful yet accessible means to characterize the unfolding/refolding dynamics of individual molecules and resolve closely spaced, transiently occupied folding intermediates. On a modern commercial AFM, these applications and others are now limited by the mechanical properties of the cantilever. Specifically, AFM-based SMFS data quality is degraded by a commercial cantilever's limited combination of temporal resolution, force precision, and force stability. Recently, we modified commercial cantilevers with a focused ion beam to optimize their properties for SMFS. Here, we extend this capability by modifying a 40 × 18 μm 2 cantilever into one terminated with a gold-coated, 4 × 4 μm 2 reflective region connected to an uncoated 2-μm-wide central shaft. This "Warhammer" geometry achieved 8.5-μs resolution coupled with improved force precision and sub-pN stability over 100 s when measured on a commercial AFM. We highlighted this cantilever's biological utility by first resolving a calmodulin unfolding intermediate previously undetected by AFM and then measuring the stabilization of calmodulin by myosin light chain kinase at dramatically higher unfolding velocities than in previous AFM studies. More generally, enhancing data quality via an improved combination of time resolution, force precision, and force stability will broadly benefit biological applications of AFM. Published by Elsevier Inc.

Application of CRAFT (complete reduction to amplitude frequency table) in nonuniformly sampled (NUS) 2D NMR data processing.

PubMed

Krishnamurthy, Krish; Hari, Natarajan

2017-09-15

The recently published CRAFT (complete reduction to amplitude frequency table) technique converts the raw FID data (i.e., time domain data) into a table of frequencies, amplitudes, decay rate constants, and phases. It offers an alternate approach to decimate time-domain data, with minimal preprocessing step. It has been shown that application of CRAFT technique to process the t 1 dimension of the 2D data significantly improved the detectable resolution by its ability to analyze without the use of ubiquitous apodization of extensively zero-filled data. It was noted earlier that CRAFT did not resolve sinusoids that were not already resolvable in time-domain (i.e., t 1 max dependent resolution). We present a combined NUS-IST-CRAFT approach wherein the NUS acquisition technique (sparse sampling technique) increases the intrinsic resolution in time-domain (by increasing t 1 max), IST fills the gap in the sparse sampling, and CRAFT processing extracts the information without loss due to any severe apodization. NUS and CRAFT are thus complementary techniques to improve intrinsic and usable resolution. We show that significant improvement can be achieved with this combination over conventional NUS-IST processing. With reasonable sensitivity, the models can be extended to significantly higher t 1 max to generate an indirect-DEPT spectrum that rivals the direct observe counterpart. Copyright © 2017 John Wiley & Sons, Ltd.

Toward acquiring comprehensive radiosurgery field commissioning data using the PRESAGE®/optical-CT 3D dosimetry system

PubMed Central

Clift, Corey; Thomas, Andrew; Adamovics, John; Chang, Zheng; Das, Indra; Oldham, Mark

2010-01-01

Achieving accurate small field dosimetry is challenging. This study investigates the utility of a radiochromic plastic PRESAGE® read with optical-CT for the acquisition of radiosurgery field commissioning data from a Novalis Tx system with a high-definition multileaf collimator (HDMLC). Total scatter factors (Sc, p), beam profiles, and penumbrae were measured for five different radiosurgery fields (5, 10, 20, 30 and 40 mm) using a commercially available optical-CT scanner (OCTOPUS, MGS Research). The percent depth dose (PDD), beam profile and penumbra of the 10 mm field were also measured using a higher resolution in-house prototype CCD-based scanner. Gafchromic EBT® film was used for independent verification. Measurements of Sc, p made with PRESAGE® and film agreed with mini-ion chamber commissioning data to within 4% for every field (range 0.2–3.6% for PRESAGE®, and 1.6–3.6% for EBT). PDD, beam profile and penumbra measurements made with the two PRESAGE®/optical-CT systems and film showed good agreement with the high-resolution diode commissioning measurements with a competitive resolution (0.5 mm pixels). The in-house prototype optical-CT scanner allowed much finer resolution compared with previous applications of PRESAGE®. The advantages of the PRESAGE® system for small field dosimetry include 3D measurements, negligible volume averaging, directional insensitivity, an absence of beam perturbations, energy and dose rate independence. PMID:20134082

Comparison of fan beam, slit-slat and multi-pinhole collimators for molecular breast tomosynthesis.

PubMed

van Roosmalen, Jarno; Beekman, Freek J; Goorden, Marlies C

2018-05-16

Recently, we proposed and optimized dedicated multi-pinhole molecular breast tomosynthesis (MBT) that images a lightly compressed breast. As MBT may also be performed with other types of collimators, the aim of this paper is to optimize MBT with fan beam and slit-slat collimators and to compare its performance to that of multi-pinhole MBT to arrive at a truly optimized design. Using analytical expressions, we first optimized fan beam and slit-slat collimator parameters to reach maximum sensitivity at a series of given system resolutions. Additionally, we performed full system simulations of a breast phantom containing several tumours for the optimized designs. We found that at equal system resolution the maximum achievable sensitivity increases from pinhole to slit-slat to fan beam collimation with fan beam and slit-slat MBT having on average a 48% and 20% higher sensitivity than multi-pinhole MBT. Furthermore, by inspecting simulated images and applying a tumour-to-background contrast-to-noise (TB-CNR) analysis, we found that slit-slat collimators underperform with respect to the other collimator types. The fan beam collimators obtained a similar TB-CNR as the pinhole collimators, but the optimum was reached at different system resolutions. For fan beam collimators, a 6-8 mm system resolution was optimal in terms of TB-CNR, while with pinhole collimation highest TB-CNR was reached in the 7-10 mm range.

Extraction and labeling high-resolution images from PDF documents

NASA Astrophysics Data System (ADS)

Chachra, Suchet K.; Xue, Zhiyun; Antani, Sameer; Demner-Fushman, Dina; Thoma, George R.

2013-12-01

Accuracy of content-based image retrieval is affected by image resolution among other factors. Higher resolution images enable extraction of image features that more accurately represent the image content. In order to improve the relevance of search results for our biomedical image search engine, Open-I, we have developed techniques to extract and label high-resolution versions of figures from biomedical articles supplied in the PDF format. Open-I uses the open-access subset of biomedical articles from the PubMed Central repository hosted by the National Library of Medicine. Articles are available in XML and in publisher supplied PDF formats. As these PDF documents contain little or no meta-data to identify the embedded images, the task includes labeling images according to their figure number in the article after they have been successfully extracted. For this purpose we use the labeled small size images provided with the XML web version of the article. This paper describes the image extraction process and two alternative approaches to perform image labeling that measure the similarity between two images based upon the image intensity projection on the coordinate axes and similarity based upon the normalized cross-correlation between the intensities of two images. Using image identification based on image intensity projection, we were able to achieve a precision of 92.84% and a recall of 82.18% in labeling of the extracted images.

Silver nanowires for highly reproducible cantilever based AFM-TERS microscopy: towards a universal TERS probe.

PubMed

Walke, Peter; Fujita, Yasuhiko; Peeters, Wannes; Toyouchi, Shuichi; Frederickx, Wout; De Feyter, Steven; Uji-I, Hiroshi

2018-04-26

Tip-enhanced Raman scattering (TERS) microscopy is a unique analytical tool to provide complementary chemical and topographic information of surfaces with nanometric resolution. However, difficulties in reliably producing the necessary metallized scanning probe tips has limited its widespread utilisation, particularly in the case of cantilever-based atomic force microscopy. Attempts to alleviate tip related issues using colloidal or bottom-up engineered tips have so far not reported consistent probes for both Raman and topographic imaging. Here we demonstrate the reproducible fabrication of cantilever-based high-performance TERS probes for both topographic and Raman measurements, based on an approach that utilises noble metal nanowires as the active TERS probe. The tips show 10 times higher TERS contrasts than the most typically used electrochemically-etched tips, and show a reproducibility for TERS greater than 90%, far greater than found with standard methods. We show that TERS can be performed in tapping as well as contact AFM mode, with optical resolutions around or below 15 nm, and with a maximum resolution achieved in tapping-mode of 6 nm. Our work illustrates that superior TERS probes can be produced in a fast and cost-effective manner using simple wet-chemistry methods, leading to reliable and reproducible high-resolution and high-sensitivity TERS, and thus renders the technique applicable for a broad community.

Ultrawidefield microscope for high-speed fluorescence imaging and targeted optogenetic stimulation.

PubMed

Werley, Christopher A; Chien, Miao-Ping; Cohen, Adam E

2017-12-01

The rapid increase in the number and quality of fluorescent reporters and optogenetic actuators has yielded a powerful set of tools for recording and controlling cellular state and function. To achieve the full benefit of these tools requires improved optical systems with high light collection efficiency, high spatial and temporal resolution, and patterned optical stimulation, in a wide field of view (FOV). Here we describe our 'Firefly' microscope, which achieves these goals in a Ø6 mm FOV. The Firefly optical system is optimized for simultaneous photostimulation and fluorescence imaging in cultured cells. All but one of the optical elements are commercially available, yet the microscope achieves 10-fold higher light collection efficiency at its design magnification than the comparable commercially available microscope using the same objective. The Firefly microscope enables all-optical electrophysiology ('Optopatch') in cultured neurons with a throughput and information content unmatched by other neuronal phenotyping systems. This capability opens possibilities in disease modeling and phenotypic drug screening. We also demonstrate applications of the system to voltage and calcium recordings in human induced pluripotent stem cell derived cardiomyocytes.

Ultrawidefield microscope for high-speed fluorescence imaging and targeted optogenetic stimulation

PubMed Central

Werley, Christopher A.; Chien, Miao-Ping; Cohen, Adam E.

2017-01-01

The rapid increase in the number and quality of fluorescent reporters and optogenetic actuators has yielded a powerful set of tools for recording and controlling cellular state and function. To achieve the full benefit of these tools requires improved optical systems with high light collection efficiency, high spatial and temporal resolution, and patterned optical stimulation, in a wide field of view (FOV). Here we describe our ‘Firefly’ microscope, which achieves these goals in a Ø6 mm FOV. The Firefly optical system is optimized for simultaneous photostimulation and fluorescence imaging in cultured cells. All but one of the optical elements are commercially available, yet the microscope achieves 10-fold higher light collection efficiency at its design magnification than the comparable commercially available microscope using the same objective. The Firefly microscope enables all-optical electrophysiology (‘Optopatch’) in cultured neurons with a throughput and information content unmatched by other neuronal phenotyping systems. This capability opens possibilities in disease modeling and phenotypic drug screening. We also demonstrate applications of the system to voltage and calcium recordings in human induced pluripotent stem cell derived cardiomyocytes. PMID:29296505

Microfluidic device having an immobilized pH gradient and PAGE gels for protein separation and analysis

DOEpatents

Sommer, Gregory J.; Hatch, Anson V.; Singh, Anup K.; Wang, Ying-Chih

2012-12-11

Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. Device modifications enable inclusion of sodium dodecyl sulfate (SDS) in the second dimension. The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices.

Concept development for the ITER equatorial port visible∕infrared wide angle viewing system.

PubMed

Reichle, R; Beaumont, B; Boilson, D; Bouhamou, R; Direz, M-F; Encheva, A; Henderson, M; Huxford, R; Kazarian, F; Lamalle, Ph; Lisgo, S; Mitteau, R; Patel, K M; Pitcher, C S; Pitts, R A; Prakash, A; Raffray, R; Schunke, B; Snipes, J; Diaz, A Suarez; Udintsev, V S; Walker, C; Walsh, M

2012-10-01

The ITER equatorial port visible∕infrared wide angle viewing system concept is developed from the measurement requirements. The proposed solution situates 4 viewing systems in the equatorial ports 3, 9, 12, and 17 with 4 views each (looking at the upper target, the inner divertor, and tangentially left and right). This gives sufficient coverage. The spatial resolution of the divertor system is 2 times higher than the other views. For compensation of vacuum-vessel movements, an optical hinge concept is proposed. Compactness and low neutron streaming is achieved by orienting port plug doglegs horizontally. Calibration methods, risks, and R&D topics are outlined.

Microfluidic device having an immobilized pH gradient and page gels for protein separation and analysis

DOEpatents

Sommer, Gregory J; Hatch, Anson V; Singh, Anup K; Wang, Ying-Chih

2014-05-20

Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. Device modifications enable inclusion of sodium dodecyl sulfate (SDS) in the second dimension. The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices.

A pruning algorithm for Meta-blocking based on cumulative weight

NASA Astrophysics Data System (ADS)

Zhang, Fulin; Gao, Zhipeng; Niu, Kun

2017-08-01

Entity Resolution is an important process in data cleaning and data integration. It usually employs a blocking method to avoid the quadratic complexity work when scales to large data sets. Meta-blocking can perform better in the context of highly heterogeneous information spaces. Yet, its precision and efficiency still have room to improve. In this paper, we present a new pruning algorithm for Meta-Blocking. It can achieve a higher precision than the existing WEP algorithm at a small cost of recall. In addition, can reduce the runtime of the blocking process. We evaluate our proposed method over five real-world data sets.

Imaging of cartilage degeneration progression in vivo using ultrahigh-resolution OCT

NASA Astrophysics Data System (ADS)

Herz, Paul R.; Bourquin, Stephane; Hsiung, Pei-lin; Ko, Tony H.; Schneider, Karl; Fujimoto, James G.; Adams, Samuel, Jr.; Roberts, Mark; Patel, Nirlep; Brezinski, Mark

2003-10-01

Ultrahigh resolution OCT is used to visualize experimentally induced osteoarthritis in a rat knee model. Using a Cr4+:Forsterite laser, ultrahigh image resolutions of 5um are achieved. Progression of osteoarthritic remodeling and cartilage degeneration are quantified. The utility of OCT for the assessment of cartilage integrity is demonstrated.

Optimization and Calibration of Slat Position for a SPECT With Slit-Slat Collimator and Pixelated Detector Crystals

NASA Astrophysics Data System (ADS)

Deng, Xiao; Ma, Tianyu; Lecomte, Roger; Yao, Rutao

2011-10-01

To expand the availability of SPECT for biomedical research, we developed a SPECT imaging system on an existing animal PET detector by adding a slit-slat collimator. As the detector crystals are pixelated, the relative slat-to-crystal position (SCP) in the axial direction affects the photon flux distribution onto the crystals. The accurate knowledge of SCP is important to the axial resolution and sensitivity of the system. This work presents a method for optimizing SCP in system design and for determining SCP in system geometrical calibration. The optimization was achieved by finding the SCP that provides higher spatial resolution in terms of average-root-mean-square (R̅M̅S̅) width of the axial point spread function (PSF) without loss of sensitivity. The calibration was based on the least-square-error method that minimizes the difference between the measured and modeled axial point spread projections. The uniqueness and accuracy of the calibration results were validated through a singular value decomposition (SVD) based approach. Both the optimization and calibration techniques were evaluated with Monte Carlo (MC) simulated data. We showed that the [R̅M̅S̅] was improved about 15% with the optimal SCP as compared to the least-optimal SCP, and system sensitivity was not affected by SCP. The SCP error achieved by the proposed calibration method was less than 0.04 mm. The calibrated SCP value was used in MC simulation to generate the system matrix which was used for image reconstruction. The images of simulated phantoms showed the expected resolution performance and were artifact free. We conclude that the proposed optimization and calibration method is effective for the slit-slat collimator based SPECT systems.

High Spatiotemporal Resolution ECoG Recording of Somatosensory Evoked Potentials with Flexible Micro-Electrode Arrays.

PubMed

Kaiju, Taro; Doi, Keiichi; Yokota, Masashi; Watanabe, Kei; Inoue, Masato; Ando, Hiroshi; Takahashi, Kazutaka; Yoshida, Fumiaki; Hirata, Masayuki; Suzuki, Takafumi

2017-01-01

Electrocorticogram (ECoG) has great potential as a source signal, especially for clinical BMI. Until recently, ECoG electrodes were commonly used for identifying epileptogenic foci in clinical situations, and such electrodes were low-density and large. Increasing the number and density of recording channels could enable the collection of richer motor/sensory information, and may enhance the precision of decoding and increase opportunities for controlling external devices. Several reports have aimed to increase the number and density of channels. However, few studies have discussed the actual validity of high-density ECoG arrays. In this study, we developed novel high-density flexible ECoG arrays and conducted decoding analyses with monkey somatosensory evoked potentials (SEPs). Using MEMS technology, we made 96-channel Parylene electrode arrays with an inter-electrode distance of 700 μm and recording site area of 350 μm 2 . The arrays were mainly placed onto the finger representation area in the somatosensory cortex of the macaque, and partially inserted into the central sulcus. With electrical finger stimulation, we successfully recorded and visualized finger SEPs with a high spatiotemporal resolution. We conducted offline analyses in which the stimulated fingers and intensity were predicted from recorded SEPs using a support vector machine. We obtained the following results: (1) Very high accuracy (~98%) was achieved with just a short segment of data (~15 ms from stimulus onset). (2) High accuracy (~96%) was achieved even when only a single channel was used. This result indicated placement optimality for decoding. (3) Higher channel counts generally improved prediction accuracy, but the efficacy was small for predictions with feature vectors that included time-series information. These results suggest that ECoG signals with high spatiotemporal resolution could enable greater decoding precision or external device control.

High Spatiotemporal Resolution ECoG Recording of Somatosensory Evoked Potentials with Flexible Micro-Electrode Arrays

PubMed Central

Kaiju, Taro; Doi, Keiichi; Yokota, Masashi; Watanabe, Kei; Inoue, Masato; Ando, Hiroshi; Takahashi, Kazutaka; Yoshida, Fumiaki; Hirata, Masayuki; Suzuki, Takafumi

2017-01-01

Electrocorticogram (ECoG) has great potential as a source signal, especially for clinical BMI. Until recently, ECoG electrodes were commonly used for identifying epileptogenic foci in clinical situations, and such electrodes were low-density and large. Increasing the number and density of recording channels could enable the collection of richer motor/sensory information, and may enhance the precision of decoding and increase opportunities for controlling external devices. Several reports have aimed to increase the number and density of channels. However, few studies have discussed the actual validity of high-density ECoG arrays. In this study, we developed novel high-density flexible ECoG arrays and conducted decoding analyses with monkey somatosensory evoked potentials (SEPs). Using MEMS technology, we made 96-channel Parylene electrode arrays with an inter-electrode distance of 700 μm and recording site area of 350 μm2. The arrays were mainly placed onto the finger representation area in the somatosensory cortex of the macaque, and partially inserted into the central sulcus. With electrical finger stimulation, we successfully recorded and visualized finger SEPs with a high spatiotemporal resolution. We conducted offline analyses in which the stimulated fingers and intensity were predicted from recorded SEPs using a support vector machine. We obtained the following results: (1) Very high accuracy (~98%) was achieved with just a short segment of data (~15 ms from stimulus onset). (2) High accuracy (~96%) was achieved even when only a single channel was used. This result indicated placement optimality for decoding. (3) Higher channel counts generally improved prediction accuracy, but the efficacy was small for predictions with feature vectors that included time-series information. These results suggest that ECoG signals with high spatiotemporal resolution could enable greater decoding precision or external device control. PMID:28442997

Ultrasonic superlensing jets and acoustic-fork sheets

NASA Astrophysics Data System (ADS)

Mitri, F. G.

2017-05-01

Focusing acoustical (and optical) beams beyond the diffraction limit has remained a major challenge in imaging instruments and systems, until recent advances on ;hyper; or ;super; lensing and higher-resolution imaging techniques have shown the counterintuitive violation of this rule under certain circumstances. Nonetheless, the proposed technologies of super-resolution acoustical focusing beyond the diffraction barrier require complex tools such as artificially engineered metamaterials, and other hardware equipment that may not be easily synthesized or manufactured. The present contribution therefore suggests a simple and reliable method of using a sound-penetrable circular cylinder lens illuminated by a nonparaxial Gaussian acoustical sheet (i.e. finite beam in 2D) to produce non-evanescent ultrasonic superlensing jets (or bullets) and acoustical 'snail-fork' shaped wavefronts with limited diffraction. The generalized (near-field) scattering theory for acoustical sheets of arbitrary wavefronts and incidence is utilized to synthesize the incident beam based upon the angular spectrum decomposition method and the multipole expansion method in cylindrical wave functions to compute the scattered pressure around the cylinder with particular emphasis on its physical properties. The results show that depending on the beam and lens parameters, a tight focusing (with dimensions much smaller than the beam waist) can be achieved. Subwavelength resolution can be also achieved by selecting a lens material with a speed of sound exceeding that of the host fluid medium. The ultrasonic superlensing jets provide the impetus to develop improved subwavelength microscopy and acoustical image-slicing systems, cell lysis and surgery, and photoacoustic imaging to name a few examples. Moreover, an acoustical fork-sheet generation may open innovative avenues in reconfigurable on-chip micro/nanoparticle tweezers and surface acoustic waves devices.

Study of sub-pixel position resolution with time-correlated transient signals in 3D pixelated CdZnTe detectors with varying pixel sizes

NASA Astrophysics Data System (ADS)

Ocampo Giraldo, L.; Bolotnikov, A. E.; Camarda, G. S.; De Geronimo, G.; Fried, J.; Gul, R.; Hodges, D.; Hossain, A.; Ünlü, K.; Vernon, E.; Yang, G.; James, R. B.

2018-03-01

We evaluated the sub-pixel position resolution achievable in large-volume CdZnTe pixelated detectors with conventional pixel patterns and for several different pixel sizes: 2.8 mm, 1.72 mm, 1.4 mm and 0.8 mm. Achieving position resolution below the physical dimensions of pixels (sub-pixel resolution) is a practical path for making high-granularity position-sensitive detectors, <100 μm, using a limited number of pixels dictated by the mechanical constraints and multi-channel readout electronics. High position sensitivity is important for improving the imaging capability of CZT gamma cameras. It also allows for making more accurate corrections of response non-uniformities caused by crystal defects, thus enabling use of standard-grade (unselected) and less expensive CZT crystals for producing large-volume position-sensitive CZT detectors feasible for many practical applications. We analyzed the digitized charge signals from a representative 9 pixels and the cathode, generated using a pulsed-laser light beam focused down to 10 μm (650 nm) to scan over a selected 3 × 3 pixel area. We applied our digital pulse processing technique to the time-correlated signals captured from adjacent pixels to achieve and evaluate the capability for sub-pixel position resolution. As an example, we also demonstrated an application of 3D corrections to improve the energy resolution and positional information of the events for the tested detectors.

Study of sub-pixel position resolution with time-correlated transient signals in 3D pixelated CdZnTe detectors with varying pixel sizes

DOE PAGES

Giraldo, L. Ocampo; Bolotnikov, A. E.; Camarda, G. S.; ...

2017-12-18

Here, we evaluated the sub-pixel position resolution achievable in large-volume CdZnTe pixelated detectors with conventional pixel patterns and for several different pixel sizes: 2.8 mm, 1.72 mm, 1.4 mm and 0.8 mm. Achieving position resolution below the physical dimensions of pixels (sub-pixel resolution) is a practical path for making high-granularity position-sensitive detectors, <100 μμm, using a limited number of pixels dictated by the mechanical constraints and multi-channel readout electronics. High position sensitivity is important for improving the imaging capability of CZT gamma cameras. It also allows for making more accurate corrections of response non-uniformities caused by crystal defects, thus enablingmore » use of standard-grade (unselected) and less expensive CZT crystals for producing large-volume position-sensitive CZT detectors feasible for many practical applications. We analyzed the digitized charge signals from a representative 9 pixels and the cathode, generated using a pulsed-laser light beam focused down to 10 m (650 nm) to scan over a selected 3×3 pixel area. We applied our digital pulse processing technique to the time-correlated signals captured from adjacent pixels to achieve and evaluate the capability for sub-pixel position resolution. As an example, we also demonstrated an application of 3D corrections to improve the energy resolution and positional information of the events for the tested detectors.« less

Fundamental limits to superresolution fluorescence microscopy

NASA Astrophysics Data System (ADS)

Small, Alex

2013-02-01

Superresolution fluorescence microscopy techniques such as PALM, STORM, STED, and Structured Illumination Microscopy (SIM) enable imaging of live cells at nanometer resolution. The common theme in all of these techniques is that the diffraction limit is circumvented by controlling the states of fluorescent molecules. Although the samples are labeled very densely (i.e. with spacing much smaller than the Airy distance), not all of the molecules are emitting at the same time. Consequently, one does not encounter overlapping blurs. In the deterministic techniques (STED, SIM) the achievable resolution scales as the wavelength of light divided by the square root of the intensity of a beam used to control the fluorescent state. In the stochastic techniques (PALM, STORM), the achievable resolution scales as the wavelength of light divided by the square root of the number of photons collected. Although these limits arise from very different mechanisms (parabolic beam profiles for STED and SIM, statistics for PALM and STORM), in all cases the resolution scales inversely with the square root of a measure of the number of photons used in the experiment. We have developed a proof that this relationship between resolution and photon count is universal to techniques that control the states of fluorophores using classical light. Our proof encompasses linear and nonlinear optics, as well as computational post-processing techniques for extracting information beyond the diffraction limit. If there are techniques that can achieve a more efficient relationship between resolution and photon count, those techniques will require light exhibiting non-classical correlations.

CMOS time-to-digital converter based on a pulse-mixing scheme

NASA Astrophysics Data System (ADS)

Chen, Chun-Chi; Hwang, Chorng-Sii; Liu, Keng-Chih; Chen, Guan-Hong

2014-11-01

This paper proposes a new pulse-mixing scheme utilizing both pulse-shrinking and pulse-stretching mechanisms to improve the performance of time-to-digital converters (TDCs). The temporal resolution of the conventional pulse-shrinking mechanism is determined by the size ratio between homogeneous and inhomogeneous elements. The proposed scheme which features double-stage operation derives its resolution according to the time difference between pulse-shrinking and pulse-stretching amounts. Thus, it can achieve greater immunity against temperature and ambient variations than that of the single-stage scheme. The circuit area also can be reduced by the proposed pulse-mixing scheme. In addition, this study proposes an improved cyclic delay line to eliminate the undesirable shift in the temporal resolution successfully. Therefore, the effective resolution can be controlled completely by the pulse-mixing unit to improve accuracy. The proposed TDC composed of only one cyclic delay line and one counter is fabricated in a TSMC CMOS 0.35-μm DPQM process. The chip core occupies an extremely small area of 0.02 mm2, which is the best among the related works. The experimental result shows that an effective resolution of around 53 ps within ±13% variation over a 0-100 °C temperature range is achieved. The power consumption is 90 μW at a sample rate of 1000 samples/s. In addition to the reduced area, the proposed TDC circuit achieves its resolution with less thermal-sensitivity and better fluctuations caused by process variations.

Target-oriented retrieval of subsurface wave fields - Pushing the resolution limits in seismic imaging

NASA Astrophysics Data System (ADS)

Vasconcelos, Ivan; Ozmen, Neslihan; van der Neut, Joost; Cui, Tianci

2017-04-01

Travelling wide-bandwidth seismic waves have long been used as a primary tool in exploration seismology because they can probe the subsurface over large distances, while retaining relatively high spatial resolution. The well-known Born resolution limit often seems to be the lower bound on spatial imaging resolution in real life examples. In practice, data acquisition cost, time constraints and other factors can worsen the resolution achieved by wavefield imaging. Could we obtain images whose resolution beats the Born limits? Would it be practical to achieve it, and what are we missing today to achieve this? In this talk, we will cover aspects of linear and nonlinear seismic imaging to understand elements that play a role in obtaining "super-resolved" seismic images. New redatuming techniques, such as the Marchenko method, enable the retrieval of subsurface fields that include multiple scattering interactions, while requiring relatively little knowledge of model parameters. Together with new concepts in imaging, such as Target-Enclosing Extended Images, these new redatuming methods enable new targeted imaging frameworks. We will make a case as to why target-oriented approaches to reconstructing subsurface-domain wavefields from surface data may help in increasing the resolving power of seismic imaging, and in pushing the limits on parameter estimation. We will illustrate this using a field data example. Finally, we will draw connections between seismic and other imaging modalities, and discuss how this framework could be put to use in other applications

Study of sub-pixel position resolution with time-correlated transient signals in 3D pixelated CdZnTe detectors with varying pixel sizes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Giraldo, L. Ocampo; Bolotnikov, A. E.; Camarda, G. S.

Here, we evaluated the sub-pixel position resolution achievable in large-volume CdZnTe pixelated detectors with conventional pixel patterns and for several different pixel sizes: 2.8 mm, 1.72 mm, 1.4 mm and 0.8 mm. Achieving position resolution below the physical dimensions of pixels (sub-pixel resolution) is a practical path for making high-granularity position-sensitive detectors, <100 μμm, using a limited number of pixels dictated by the mechanical constraints and multi-channel readout electronics. High position sensitivity is important for improving the imaging capability of CZT gamma cameras. It also allows for making more accurate corrections of response non-uniformities caused by crystal defects, thus enablingmore » use of standard-grade (unselected) and less expensive CZT crystals for producing large-volume position-sensitive CZT detectors feasible for many practical applications. We analyzed the digitized charge signals from a representative 9 pixels and the cathode, generated using a pulsed-laser light beam focused down to 10 m (650 nm) to scan over a selected 3×3 pixel area. We applied our digital pulse processing technique to the time-correlated signals captured from adjacent pixels to achieve and evaluate the capability for sub-pixel position resolution. As an example, we also demonstrated an application of 3D corrections to improve the energy resolution and positional information of the events for the tested detectors.« less

A High Resolution Liquid Xenon Imaging Telescope for 0.3-10 MeV Gamma Ray Astrophysics: Construction and Initial Balloon Flights

NASA Technical Reports Server (NTRS)

Aprile, Elena

1993-01-01

The results achieved with a 3.5 liter liquid xenon time projection chamber (LXe-TPC) prototype during the first year include: the efficiency of detecting the primary scintillation light for event triggering has been measured to be higher than 85%; the charge response has been measured to be stable to within 0.1% for a period of time of about 30 hours; the electron lifetime has been measured to be in excess of 1.3 ms; the energy resolution has been measured to be consistent with previous results obtained with small volume chambers; X-Y gamma ray imaging has been demonstrated with a nondestructive orthogonal wires readout; Monte Carlo simulation results on detection efficiency, expected background count rate at balloon altitude, background reduction algorithms, telescope response to point-like and diffuse sources, and polarization sensitivity calculations; and work on a 10 liter LXe-TPC prototype and gas purification/recovery system.

Reliability of Fault Tolerant Control Systems. Part 2

NASA Technical Reports Server (NTRS)

Wu, N. Eva

2000-01-01

This paper reports Part II of a two part effort that is intended to delineate the relationship between reliability and fault tolerant control in a quantitative manner. Reliability properties peculiar to fault-tolerant control systems are emphasized, such as the presence of analytic redundancy in high proportion, the dependence of failures on control performance, and high risks associated with decisions in redundancy management due to multiple sources of uncertainties and sometimes large processing requirements. As a consequence, coverage of failures through redundancy management can be severely limited. The paper proposes to formulate the fault tolerant control problem as an optimization problem that maximizes coverage of failures through redundancy management. Coverage modeling is attempted in a way that captures its dependence on the control performance and on the diagnostic resolution. Under the proposed redundancy management policy, it is shown that an enhanced overall system reliability can be achieved with a control law of a superior robustness, with an estimator of a higher resolution, and with a control performance requirement of a lesser stringency.

Note: An absolute X-Y-Θ position sensor using a two-dimensional phase-encoded binary scale

NASA Astrophysics Data System (ADS)

Kim, Jong-Ahn; Kim, Jae Wan; Kang, Chu-Shik; Jin, Jonghan

2018-04-01

This Note presents a new absolute X-Y-Θ position sensor for measuring planar motion of a precision multi-axis stage system. By analyzing the rotated image of a two-dimensional phase-encoded binary scale (2D), the absolute 2D position values at two separated points were obtained and the absolute X-Y-Θ position could be calculated combining these values. The sensor head was constructed using a board-level camera, a light-emitting diode light source, an imaging lens, and a cube beam-splitter. To obtain the uniform intensity profiles from the vignette scale image, we selected the averaging directions deliberately, and higher resolution in the angle measurement could be achieved by increasing the allowable offset size. The performance of a prototype sensor was evaluated in respect of resolution, nonlinearity, and repeatability. The sensor could resolve 25 nm linear and 0.001° angular displacements clearly, and the standard deviations were less than 18 nm when 2D grid positions were measured repeatedly.

Modeling of charged particles trajectories in order to optimize the design of a new, higher resolution, Time of flight- Positron Annihilation Induced Auger Electron Spectroscopy (TOF PAES) System

NASA Astrophysics Data System (ADS)

Joglekar, Prasad; Lim, L.; Satyal, Suman; Kalaskar, Sushant; Shastry, K.; Weiss, Alex

2011-03-01

Time of Flight Positron Annihilation Induced~Auger Electron Spectroscopy~(TOF PAES) is a surface analytical technique with high surface selectivity. TOF PAES is used to study elemental composition, surface defects, and various energy loss mechanisms. Positrons incident on the sample surface at low energies can be trapped in an image-potential well just above the surface Prior to annihilation. Consequently it is possible to use positron annihilation related signals to selectively probe the top-most atomic layer. This poster presents the results of modeling of the charge particle beam transport system performed in connection with the optimization of the the design of the new TOF-PAES system currently under construction at U T Arlington. The system will incorporate a 2 m long drift tube in order to achieve better energy resolution than our previous TOF-PAES system design which used a 1 m long drift tube NSF DMR 0907679, Welch Foundation Y 1100.

Spectrocopic measurements of water vapor plasmas at high resolution: The optical transition probabilities for OH (A 2 Sigma - X 2 Pi)

NASA Technical Reports Server (NTRS)

Klein, L.

1972-01-01

Emission and absorption spectra of water vapor plasmas generated in a wall-stabilized arc at atmospheric pressure and 4 current, and at 0.03 atm and 15 to 50 A, were measured at high spatial and spectral resolution. The gas temperature was determined from the shape of Doppler-broadened rotational lines of OH. The observed nonequilibrium population distributions over the energy levels of atoms are interpreted in terms of a theoretical state model for diffusion-controlled arc plasmas. Excellent correlation is achieved between measured and predicted occupation of hydrogen energy levels. It is shown that the population distribution over the nonpredissociating rotational-vibrational levels of the A 2 Sigma state of OH is close to an equilibrium distribution at the gas temperature, although the total density of this state is much higher than its equilibrium density. The reduced intensities of the rotational lines originating in these levels yielded Boltzmann plots that were strictly linear.

An Automated Parallel Image Registration Technique Based on the Correlation of Wavelet Features

NASA Technical Reports Server (NTRS)

LeMoigne, Jacqueline; Campbell, William J.; Cromp, Robert F.; Zukor, Dorothy (Technical Monitor)

2001-01-01

With the increasing importance of multiple platform/multiple remote sensing missions, fast and automatic integration of digital data from disparate sources has become critical to the success of these endeavors. Our work utilizes maxima of wavelet coefficients to form the basic features of a correlation-based automatic registration algorithm. Our wavelet-based registration algorithm is tested successfully with data from the National Oceanic and Atmospheric Administration (NOAA) Advanced Very High Resolution Radiometer (AVHRR) and the Landsat/Thematic Mapper(TM), which differ by translation and/or rotation. By the choice of high-frequency wavelet features, this method is similar to an edge-based correlation method, but by exploiting the multi-resolution nature of a wavelet decomposition, our method achieves higher computational speeds for comparable accuracies. This algorithm has been implemented on a Single Instruction Multiple Data (SIMD) massively parallel computer, the MasPar MP-2, as well as on the CrayT3D, the Cray T3E and a Beowulf cluster of Pentium workstations.

Speckle reduction in optical coherence tomography using two-step iteration method (Conference Presentation)

NASA Astrophysics Data System (ADS)

Wang, Xianghong; Liu, Xinyu; Wang, Nanshuo; Yu, Xiaojun; Bo, En; Chen, Si; Liu, Linbo

2017-02-01

Optical coherence tomography (OCT) provides high resolution and cross-sectional images of biological tissue and is widely used for diagnosis of ocular diseases. However, OCT images suffer from speckle noise, which typically considered as multiplicative noise in nature, reducing the image resolution and contrast. In this study, we propose a two-step iteration (TSI) method to suppress those noises. We first utilize augmented Lagrange method to recover a low-rank OCT image and remove additive Gaussian noise, and then employ the simple and efficient split Bregman method to solve the Total-Variation Denoising model. We validated such proposed method using images of swine, rabbit and human retina. Results demonstrate that our TSI method outperforms the other popular methods in achieving higher peak signal-to-noise ratio (PSNR) and structure similarity (SSIM) while preserving important structural details, such as tiny capillaries and thin layers in retinal OCT images. In addition, the results of our TSI method show clearer boundaries and maintains high image contrast, which facilitates better image interpretations and analyses.

Apparatus and method to achieve high-resolution microscopy with non-diffracting or refracting radiation

DOEpatents

Tobin, Jr., Kenneth W.; Bingham, Philip R.; Hawari, Ayman I.

2012-11-06

An imaging system employing a coded aperture mask having multiple pinholes is provided. The coded aperture mask is placed at a radiation source to pass the radiation through. The radiation impinges on, and passes through an object, which alters the radiation by absorption and/or scattering. Upon passing through the object, the radiation is detected at a detector plane to form an encoded image, which includes information on the absorption and/or scattering caused by the material and structural attributes of the object. The encoded image is decoded to provide a reconstructed image of the object. Because the coded aperture mask includes multiple pinholes, the radiation intensity is greater than a comparable system employing a single pinhole, thereby enabling a higher resolution. Further, the decoding of the encoded image can be performed to generate multiple images of the object at different distances from the detector plane. Methods and programs for operating the imaging system are also disclosed.

Approach for discrimination and quantification of electroactive species: kinetics difference revealed by higher harmonics of Fourier transformed sinusoidal voltammetry.

PubMed

Fang, Yishan; Huang, Xinjian; Wang, Lishi

2015-01-06

Discrimination and quantification of electroactive species are traditionally realized by a potential difference which is mainly determined by thermodynamics. However, the resolution of this approach is limited to tens of millivolts. In this paper, we described an application of Fourier transformed sinusoidal voltammetry (FT-SV) that provides a new approach for discrimination and quantitative evaluation of electroactive species, especially thermodynamic similar ones. Numerical simulation indicates that electron transfer kinetics difference between electroactive species can be revealed by the phase angle of higher order harmonics of FT-SV, and the difference can be amplified order by order. Thus, even a very subtle kinetics difference can be amplified to be distinguishable at a certain order of harmonics. This method was verified with structurally similar ferrocene derivatives which were chosen as the model systems. Although these molecules have very close redox potential (<10 mV), discrimination and selective detection were achieved by as high as the thirteenth harmonics. The results demonstrated the feasibility and reliability of the method. It was also implied that the combination of the traditional thermodynamic method and this kinetics method can form a two-dimension resolved detection method, and it has the potential to extend the resolution of voltammetric techniques to a new level.

Detecting Weak Spectral Lines in Interferometric Data through Matched Filtering

NASA Astrophysics Data System (ADS)

Loomis, Ryan A.; Öberg, Karin I.; Andrews, Sean M.; Walsh, Catherine; Czekala, Ian; Huang, Jane; Rosenfeld, Katherine A.

2018-04-01

Modern radio interferometers enable observations of spectral lines with unprecedented spatial resolution and sensitivity. In spite of these technical advances, many lines of interest are still at best weakly detected and therefore necessitate detection and analysis techniques specialized for the low signal-to-noise ratio (S/N) regime. Matched filters can leverage knowledge of the source structure and kinematics to increase sensitivity of spectral line observations. Application of the filter in the native Fourier domain improves S/N while simultaneously avoiding the computational cost and ambiguities associated with imaging, making matched filtering a fast and robust method for weak spectral line detection. We demonstrate how an approximate matched filter can be constructed from a previously observed line or from a model of the source, and we show how this filter can be used to robustly infer a detection significance for weak spectral lines. When applied to ALMA Cycle 2 observations of CH3OH in the protoplanetary disk around TW Hya, the technique yields a ≈53% S/N boost over aperture-based spectral extraction methods, and we show that an even higher boost will be achieved for observations at higher spatial resolution. A Python-based open-source implementation of this technique is available under the MIT license at http://github.com/AstroChem/VISIBLE.

High-Bandwidth Dynamic Full-Field Profilometry for Nano-Scale Characterization of MEMS

NASA Astrophysics Data System (ADS)

Chen, Liang-Chia; Huang, Yao-Ting; Chang, Pi-Bai

2006-10-01

The article describes an innovative optical interferometric methodology to delivery dynamic surface profilometry with a measurement bandwidth up to 10MHz or higher and a vertical resolution up to 1 nm. Previous work using stroboscopic microscopic interferometry for dynamic characterization of micro (opto)electromechanical systems (M(O)EMS) has been limited in measurement bandwidth mainly within a couple of MHz. For high resonant mode analysis, the stroboscopic light pulse is insufficiently short to capture the moving fringes from dynamic motion of the detected structure. In view of this need, a microscopic prototype based on white-light stroboscopic interferometry with an innovative light superposition strategy was developed to achieve dynamic full-field profilometry with a high measurement bandwidth up to 10MHz or higher. The system primarily consists of an optical microscope, on which a Mirau interferometric objective embedded with a piezoelectric vertical translator, a high-power LED light module with dual operation modes and light synchronizing electronics unit are integrated. A micro cantilever beam used in AFM was measured to verify the system capability in accurate characterisation of dynamic behaviours of the device. The full-field seventh-mode vibration at a vibratory frequency of 3.7MHz can be fully characterized and nano-scale vertical measurement resolution as well as tens micrometers of vertical measurement range can be performed.

Using SCIAMACHY and Ground-based FTS Measurements to Test the OCO X(sub CO2) Retrieval and Validation Approach

NASA Technical Reports Server (NTRS)

Boesch, Hartmut; Toon, G.; Sen, B; Li, Q. B.; Salawitch, R.; Miller, C.; Crisp, D.; Washenfelder, R.; Wennberg, P.; Natraj, V.;

Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging

PubMed Central

Shimozawa, Togo; Yamagata, Kazuo; Kondo, Takefumi; Hayashi, Shigeo; Shitamukai, Atsunori; Konno, Daijiro; Matsuzaki, Fumio; Takayama, Jun; Onami, Shuichi; Nakayama, Hiroshi; Kosugi, Yasuhito; Watanabe, Tomonobu M.; Fujita, Katsumasa; Mimori-Kiyosue, Yuko

2013-01-01

A recent key requirement in life sciences is the observation of biological processes in their natural in vivo context. However, imaging techniques that allow fast imaging with higher resolution in 3D thick specimens are still limited. Spinning disk confocal microscopy using a Yokogawa Confocal Scanner Unit, which offers high-speed multipoint confocal live imaging, has been found to have wide utility among cell biologists. A conventional Confocal Scanner Unit configuration, however, is not optimized for thick specimens, for which the background noise attributed to “pinhole cross-talk,” which is unintended pinhole transmission of out-of-focus light, limits overall performance in focal discrimination and reduces confocal capability. Here, we improve spinning disk confocal microscopy by eliminating pinhole cross-talk. First, the amount of pinhole cross-talk is reduced by increasing the interpinhole distance. Second, the generation of out-of-focus light is prevented by two-photon excitation that achieves selective-plane illumination. We evaluate the effect of these modifications and test the applicability to the live imaging of green fluorescent protein-expressing model animals. As demonstrated by visualizing the fine details of the 3D cell shape and submicron-size cytoskeletal structures inside animals, these strategies dramatically improve higher-resolution intravital imaging. PMID:23401517

Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging.

PubMed

Shimozawa, Togo; Yamagata, Kazuo; Kondo, Takefumi; Hayashi, Shigeo; Shitamukai, Atsunori; Konno, Daijiro; Matsuzaki, Fumio; Takayama, Jun; Onami, Shuichi; Nakayama, Hiroshi; Kosugi, Yasuhito; Watanabe, Tomonobu M; Fujita, Katsumasa; Mimori-Kiyosue, Yuko

2013-02-26

A recent key requirement in life sciences is the observation of biological processes in their natural in vivo context. However, imaging techniques that allow fast imaging with higher resolution in 3D thick specimens are still limited. Spinning disk confocal microscopy using a Yokogawa Confocal Scanner Unit, which offers high-speed multipoint confocal live imaging, has been found to have wide utility among cell biologists. A conventional Confocal Scanner Unit configuration, however, is not optimized for thick specimens, for which the background noise attributed to "pinhole cross-talk," which is unintended pinhole transmission of out-of-focus light, limits overall performance in focal discrimination and reduces confocal capability. Here, we improve spinning disk confocal microscopy by eliminating pinhole cross-talk. First, the amount of pinhole cross-talk is reduced by increasing the interpinhole distance. Second, the generation of out-of-focus light is prevented by two-photon excitation that achieves selective-plane illumination. We evaluate the effect of these modifications and test the applicability to the live imaging of green fluorescent protein-expressing model animals. As demonstrated by visualizing the fine details of the 3D cell shape and submicron-size cytoskeletal structures inside animals, these strategies dramatically improve higher-resolution intravital imaging.

Research on the shortwave infrared hyperspectral imaging technology based on Integrated Stepwise filter

NASA Astrophysics Data System (ADS)

Wei, Liqing; Xiao, Xizhong; Wang, Yueming; Zhuang, Xiaoqiong; Wang, Jianyu

2017-11-01

Space-borne hyperspectral imagery is an important tool for earth sciences and industrial applications. Higher spatial and spectral resolutions have been sought persistently, although this results in more power, larger volume and weight during a space-borne spectral imager design. For miniaturization of hyperspectral imager and optimization of spectral splitting methods, several methods are compared in this paper. Spectral time delay integration (TDI) method with high transmittance Integrated Stepwise Filter (ISF) is proposed.With the method, an ISF imaging spectrometer with TDI could achieve higher system sensitivity than the traditional prism/grating imaging spectrometer. In addition, the ISF imaging spectrometer performs well in suppressing infrared background radiation produced by instrument. A compact shortwave infrared (SWIR) hyperspectral imager prototype based on HgCdTe covering the spectral range of 2.0-2.5 μm with 6 TDI stages was designed and integrated. To investigate the performance of ISF spectrometer, a method to derive the optimal blocking band curve of the ISF is introduced, along with known error characteristics. To assess spectral performance of the ISF system, a new spectral calibration based on blackbody radiation with temperature scanning is proposed. The results of the imaging experiment showed the merits of ISF. ISF has great application prospects in the field of high sensitivity and high resolution space-borne hyperspectral imagery.

Time multiplexing super-resolution nanoscopy based on the Brownian motion of gold nanoparticles

NASA Astrophysics Data System (ADS)

Ilovitsh, Tali; Ilovitsh, Asaf; Wagner, Omer; Zalevsky, Zeev

2017-02-01

Super-resolution localization microscopy can overcome the diffraction limit and achieve a tens of order improvement in resolution. It requires labeling the sample with fluorescent probes followed with their repeated cycles of activation and photobleaching. This work presents an alternative approach that is free from direct labeling and does not require the activation and photobleaching cycles. Fluorescently labeled gold nanoparticles in a solution are distributed on top of the sample. The nanoparticles move in a random Brownian motion, and interact with the sample. By obscuring different areas in the sample, the nanoparticles encode the sub-wavelength features. A sequence of images of the sample is captured and decoded by digital post processing to create the super-resolution image. The achievable resolution is limited by the additive noise and the size of the nanoparticles. Regular nanoparticles with diameter smaller than 100nm are barely seen in a conventional bright field microscope, thus fluorescently labeled gold nanoparticles were used, with proper

Resolution of co-eluting compounds of Cannabis Sativa in comprehensive two-dimensional gas chromatography/mass spectrometry detection with Multivariate Curve Resolution-Alternating Least Squares.

PubMed

Omar, Jone; Olivares, Maitane; Amigo, José Manuel; Etxebarria, Nestor

2014-04-01

Comprehensive Two Dimensional Gas Chromatography - Mass Spectrometry (GC × GC/qMS) analysis of Cannabis sativa extracts shows a high complexity due to the large variety of terpenes and cannabinoids and to the fact that the complete resolution of the peaks is not straightforwardly achieved. In order to support the resolution of the co-eluted peaks in the sesquiterpene and the cannabinoid chromatographic region the combination of Multivariate Curve Resolution and Alternating Least Squares algorithms was satisfactorily applied. As a result, four co-eluting areas were totally resolved in the sesquiterpene region and one in the cannabinoid region in different samples of Cannabis sativa. The comparison of the mass spectral profiles obtained for each resolved peak with theoretical mass spectra allowed the identification of some of the co-eluted peaks. Finally, the classification of the studied samples was achieved based on the relative concentrations of the resolved peaks. Copyright © 2014 Elsevier B.V. All rights reserved.

Nanometric depth resolution from multi-focal images in microscopy.

PubMed

Dalgarno, Heather I C; Dalgarno, Paul A; Dada, Adetunmise C; Towers, Catherine E; Gibson, Gavin J; Parton, Richard M; Davis, Ilan; Warburton, Richard J; Greenaway, Alan H

2011-07-06

We describe a method for tracking the position of small features in three dimensions from images recorded on a standard microscope with an inexpensive attachment between the microscope and the camera. The depth-measurement accuracy of this method is tested experimentally on a wide-field, inverted microscope and is shown to give approximately 8 nm depth resolution, over a specimen depth of approximately 6 µm, when using a 12-bit charge-coupled device (CCD) camera and very bright but unresolved particles. To assess low-flux limitations a theoretical model is used to derive an analytical expression for the minimum variance bound. The approximations used in the analytical treatment are tested using numerical simulations. It is concluded that approximately 14 nm depth resolution is achievable with flux levels available when tracking fluorescent sources in three dimensions in live-cell biology and that the method is suitable for three-dimensional photo-activated localization microscopy resolution. Sub-nanometre resolution could be achieved with photon-counting techniques at high flux levels.

Nanometric depth resolution from multi-focal images in microscopy

PubMed Central

Dalgarno, Heather I. C.; Dalgarno, Paul A.; Dada, Adetunmise C.; Towers, Catherine E.; Gibson, Gavin J.; Parton, Richard M.; Davis, Ilan; Warburton, Richard J.; Greenaway, Alan H.

2011-01-01

We describe a method for tracking the position of small features in three dimensions from images recorded on a standard microscope with an inexpensive attachment between the microscope and the camera. The depth-measurement accuracy of this method is tested experimentally on a wide-field, inverted microscope and is shown to give approximately 8 nm depth resolution, over a specimen depth of approximately 6 µm, when using a 12-bit charge-coupled device (CCD) camera and very bright but unresolved particles. To assess low-flux limitations a theoretical model is used to derive an analytical expression for the minimum variance bound. The approximations used in the analytical treatment are tested using numerical simulations. It is concluded that approximately 14 nm depth resolution is achievable with flux levels available when tracking fluorescent sources in three dimensions in live-cell biology and that the method is suitable for three-dimensional photo-activated localization microscopy resolution. Sub-nanometre resolution could be achieved with photon-counting techniques at high flux levels. PMID:21247948

Scale-Resolving simulations (SRS): How much resolution do we really need?

NASA Astrophysics Data System (ADS)

Pereira, Filipe M. S.; Girimaji, Sharath

2017-11-01

Scale-resolving simulations (SRS) are emerging as the computational approach of choice for many engineering flows with coherent structures. The SRS methods seek to resolve only the most important features of the coherent structures and model the remainder of the flow field with canonical closures. With reference to a typical Large-Eddy Simulation (LES), practical SRS methods aim to resolve a considerably narrower range of scales (reduced physical resolution) to achieve an adequate degree of accuracy at reasonable computational effort. While the objective of SRS is well-founded, the criteria for establishing the optimal degree of resolution required to achieve an acceptable level of accuracy are not clear. This study considers the canonical case of the flow around a circular cylinder to address the issue of `optimal' resolution. Two important criteria are developed. The first condition addresses the issue of adequate resolution of the flow field. The second guideline provides an assessment of whether the modeled field is canonical (stochastic) turbulence amenable to closure-based computations.

High sensitivity far infrared laser diagnostics for the C-2U advanced beam-driven field-reversed configuration plasmas

DOE Office of Scientific and Technical Information (OSTI.GOV)

Deng, B. H., E-mail: bdeng@trialphaenergy.com; Beall, M.; Schroeder, J.

2016-11-15

A high sensitivity multi-channel far infrared laser diagnostics with switchable interferometry and polarimetry operation modes for the advanced neutral beam-driven C-2U field-reversed configuration (FRC) plasmas is described. The interferometer achieved superior resolution of 1 × 10{sup 16} m{sup −2} at >1.5 MHz bandwidth, illustrated by measurement of small amplitude high frequency fluctuations. The polarimetry achieved 0.04° instrument resolution and 0.1° actual resolution in the challenging high density gradient environment with >0.5 MHz bandwidth, making it suitable for weak internal magnetic field measurements in the C-2U plasmas, where the maximum Faraday rotation angle is less than 1°. The polarimetry resolution datamore » is analyzed, and high resolution Faraday rotation data in C-2U is presented together with direct evidences of field reversal in FRC magnetic structure obtained for the first time by a non-perturbative method.« less

High-resolution imaging optomechatronics for precise liquid crystal display module bonding automated optical inspection

NASA Astrophysics Data System (ADS)

Ni, Guangming; Liu, Lin; Zhang, Jing; Liu, Juanxiu; Liu, Yong

2018-01-01

With the development of the liquid crystal display (LCD) module industry, LCD modules become more and more precise with larger sizes, which demands harsh imaging requirements for automated optical inspection (AOI). Here, we report a high-resolution and clearly focused imaging optomechatronics for precise LCD module bonding AOI inspection. It first presents and achieves high-resolution imaging for LCD module bonding AOI inspection using a line scan camera (LSC) triggered by a linear optical encoder, self-adaptive focusing for the whole large imaging region using LSC, and a laser displacement sensor, which reduces the requirements of machining, assembly, and motion control of AOI devices. Results show that this system can directly achieve clearly focused imaging for AOI inspection of large LCD module bonding with 0.8 μm image resolution, 2.65-mm scan imaging width, and no limited imaging width theoretically. All of these are significant for AOI inspection in the LCD module industry and other fields that require imaging large regions with high resolution.

Analytical description of high-aperture STED resolution with 0–2π vortex phase modulation

PubMed Central

Xie, Hao; Liu, Yujia; Jin, Dayong; Santangelo, Philip J.; Xi, Peng

2014-01-01

Stimulated emission depletion (STED) can achieve optical superresolution, with the optical diffraction limit broken by the suppression on the periphery of the fluorescent focal spot. Previously, it is generally experimentally accepted that there exists an inverse square root relationship with the STED power and the resolution, but with arbitrary coefficients in expression. In this paper, we have removed the arbitrary coefficients by exploring the relationship between the STED power and the achievable resolution from vector optical theory for the widely used 0–2π vortex phase modulation. Electromagnetic fields of the focal region of a high numerical aperture objective are calculated and approximated into polynomials of radius in the focal plane, and analytical expression of resolution as a function of the STED intensity has been derived. As a result, the resolution can be estimated directly from the measurement of the saturation power of the dye and the STED power applied in the region of high STED power. PMID:24323224

Assessing the quality of rainfall data when aiming to achieve flood resilience

NASA Astrophysics Data System (ADS)

Hoang, C. T.; Tchiguirinskaia, I.; Schertzer, D.; Lovejoy, S.

2012-04-01

A new EU Floods Directive entered into force five years ago. This Directive requires Member States to coordinate adequate measures to reduce flood risk. European flood management systems require reliable rainfall statistics, e.g. the Intensity-duration-Frequency curves for shorter and shorter durations and for a larger and larger range of return periods. Preliminary studies showed that the number of floods was lower when using low time resolution data of high intensity rainfall events, compared to estimates obtained with the help of higher time resolution data. These facts suggest that a particular attention should be paid to the rainfall data quality in order to adequately investigate flood risk aiming to achieve flood resilience. The potential consequences of changes in measuring and recording techniques have been somewhat discussed in the literature with respect to a possible introduction of artificial inhomogeneities in time series. In this paper, we discuss how to detect another artificiality: most of the rainfall time series have a lower recording frequency than that is assumed, furthermore the effective high-frequency limit often depends on the recording year due to algorithm changes. This question is particularly important for operational hydrology, because an error on the effective recording high frequency introduces biases in the corresponding statistics. In this direction, we developed a first version of a SERQUAL procedure to automatically detect the effective time resolution of highly mixed data. Being applied to the 166 rainfall time series in France, the SERQUAL procedure has detected that most of them have an effective hourly resolution, rather than a 5 minutes resolution. Furthermore, series having an overall 5 minute resolution do not have it for all years. These results raise serious concerns on how to benchmark stochastic rainfall models at a sub-hourly resolution, which are particularly desirable for operational hydrology. Therefore, database quality must be checked before use. Due to the fact that the multiple scales and possible scaling behaviour of hydrological data are particularly important for many applications, including flood resilience research, this paper first investigates the sensitivity of the scaling estimates and methods to the deficit of short duration rainfall data, and consequently propose a few simple criteria for a reliable evaluation of the data quality. Then we showed that our procedure SERQUAL enable us to extract high quality sub-series from longer time series that will be much more reliable to calibrate and/or validate short duration quantiles and hydrological models.

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

PubMed Central

Young, Laurence J.; Ströhl, Florian; Kaminski, Clemens F.

2016-01-01

Optical super-resolution imaging with structured illumination microscopy (SIM) is a key technology for the visualization of processes at the molecular level in the chemical and biomedical sciences. Although commercial SIM systems are available, systems that are custom designed in the laboratory can outperform commercial systems, the latter typically designed for ease of use and general purpose applications, both in terms of imaging fidelity and speed. This article presents an in-depth guide to building a SIM system that uses total internal reflection (TIR) illumination and is capable of imaging at up to 10 Hz in three colors at a resolution reaching 100 nm. Due to the combination of SIM and TIRF, the system provides better image contrast than rival technologies. To achieve these specifications, several optical elements are used to enable automated control over the polarization state and spatial structure of the illumination light for all available excitation wavelengths. Full details on hardware implementation and control are given to achieve synchronization between excitation light pattern generation, wavelength, polarization state, and camera control with an emphasis on achieving maximum acquisition frame rate. A step-by-step protocol for system alignment and calibration is presented and the achievable resolution improvement is validated on ideal test samples. The capability for video-rate super-resolution imaging is demonstrated with living cells. PMID:27285848

High spatial resolution mapping of folds and fractures using Unmanned Aerial Vehicle (UAV) photogrammetry

NASA Astrophysics Data System (ADS)

Cruden, A. R.; Vollgger, S.

2016-12-01

The emerging capability of UAV photogrammetry combines a simple and cost-effective method to acquire digital aerial images with advanced computer vision algorithms that compute spatial datasets from a sequence of overlapping digital photographs from various viewpoints. Depending on flight altitude and camera setup, sub-centimeter spatial resolution orthophotographs and textured dense point clouds can be achieved. Orientation data can be collected for detailed structural analysis by digitally mapping such high-resolution spatial datasets in a fraction of time and with higher fidelity compared to traditional mapping techniques. Here we describe a photogrammetric workflow applied to a structural study of folds and fractures within alternating layers of sandstone and mudstone at a coastal outcrop in SE Australia. We surveyed this location using a downward looking digital camera mounted on commercially available multi-rotor UAV that autonomously followed waypoints at a set altitude and speed to ensure sufficient image overlap, minimum motion blur and an appropriate resolution. The use of surveyed ground control points allowed us to produce a geo-referenced 3D point cloud and an orthophotograph from hundreds of digital images at a spatial resolution < 10 mm per pixel, and cm-scale location accuracy. Orientation data of brittle and ductile structures were semi-automatically extracted from these high-resolution datasets using open-source software. This resulted in an extensive and statistically relevant orientation dataset that was used to 1) interpret the progressive development of folds and faults in the region, and 2) to generate a 3D structural model that underlines the complex internal structure of the outcrop and quantifies spatial variations in fold geometries. Overall, our work highlights how UAV photogrammetry can contribute to new insights in structural analysis.

Improving spatial and spectral resolution of TCV Thomson scattering

NASA Astrophysics Data System (ADS)

Hawke, J.; Andrebe, Y.; Bertizzolo, R.; Blanchard, P.; Chavan, R.; Decker, J.; Duval, B.; Lavanchy, P.; Llobet, X.; Marlétaz, B.; Marmillod, P.; Pochon, G.; Toussaint, M.

2017-12-01

The recently completed MST2 upgrade to the Thomson scattering (TS) system on TCV (Tokamak à Configuration Variable) at the Swiss Plasma Center aims to provide an enhanced spatial and spectral resolution while maintaining the high level of diagnostic flexibility for the study of TCV plasmas. The MST2 (Medium Sized Tokamak) is a work program within the Eurofusion ITER physics department, aimed at exploiting Europe's medium sized tokamak programs for a better understanding of ITER physics. This upgrade to the TCV Thomson scattering system involved the installation of 40 new compact 5-channel spectrometers and modifications to the diagnostics fiber optic design. The complete redesign of the fiber optic backplane incorporates fewer larger diameter fibers, allowing for a higher resolution in both the core and edge of TCV plasmas along the laser line, with a slight decrease in the signal to noise ratio of Thomson measurements. The 40 new spectrometers added to the system are designed to cover the full range of temperatures expected in TCV, able to measure electron temperatures (Te) with high precision between (6 eV and 20 keV) . The design of these compact spectrometers stems originally from the design utilized in the MAST (Mega Amp Spherical Tokamak) TS system located in Oxfordshire, United Kingdom. This design was implemented on TCV with an overall layout of optical fibers and spectrometers to achieve an overall increase in the spatial resolution, specifically a resolution of approximately 1% of the minor radius within the plasma pedestal region. These spectrometers also enhance the diagnostic spectral resolution, especially within the plasma edge, due to the low Te measurement capabilities. These additional spectrometers allow for a much greater diagnostic flexibility, allowing for quality full Thomson profiles in 75% of TCV plasma configurations.

Efficient high-resolution hard x-ray imaging with transparent Lu2O3:Eu scintillator thin films

NASA Astrophysics Data System (ADS)

Marton, Zsolt; Miller, Stuart R.; Brecher, Charles; Kenesei, Peter; Moore, Matthew D.; Woods, Russell; Almer, Jonathan D.; Miceli, Antonino; Nagarkar, Vivek V.

2015-09-01

We have developed microstructured Lu2O3:Eu scintillator films that provide spatial resolution on the order of micrometers for hard X-ray imaging. In addition to their outstanding resolution, Lu2O3:Eu films also exhibits both high absorption efficiency for 20 to 100 keV X-rays, and bright 610 nm emission whose intensity rivals that of the brightest known scintillators. At present, high spatial resolution of such a magnitude is achieved using ultra-thin scintillators measuring only about 1 to 5 μm in thickness, which limits absorption efficiency to ~3% for 12 keV X-rays and less than 0.1% for 20 to 100 keV X-rays; this results in excessive measurement time and exposure to the specimen. But the absorption efficiency of Lu2O3:Eu (99.9% @12 keV and 30% @ 70 keV) is much greater, significantly decreasing measurement time and radiation exposure. Our Lu2O3:Eu scintillator material, fabricated by our electron-beam physical vapor deposition (EB-PVD) process, combines superior density of 9.5 g/cm3, a microcolumnar structure for higher spatial resolution, and a bright emission (48000 photons/MeV) whose wavelength is an ideal match for the underlying CCD detector array. We grew thin films of this material on a variety of matching substrates, measuring some 5-10μm in thickness and covering areas up to 1 x 1 cm2, which can be a suitable basis for microtomography, digital radiography as well as CT and hard X-ray Micro-Tomography (XMT). The microstructure and optical transparency of such screens was optimized, and their imaging performance was evaluated in the Argonne National Laboratory's Advanced Photon Source. Spatial resolution and efficiency were also characterized.

Toward a RPC-based muon tomography system for cargo containers.

NASA Astrophysics Data System (ADS)

Baesso, P.; Cussans, D.; Thomay, C.; Velthuis, J.

2014-10-01

A large area scanner for cosmic muon tomography is currently being developed at University of Bristol. Thanks to their abundance and penetrating power, cosmic muons have been suggested as ideal candidates to scan large containers in search of special nuclear materials, which are characterized by high-Z and high density. The feasibility of such a scanner heavily depends on the detectors used to track the muons: for a typical container, the minimum required sensitive area is of the order of 100 2. The spatial resolution required depends on the geometrical configuration of the detectors. For practical purposes, a resolution of the order of 1 mm or better is desirable. A good time resolution can be exploited to provide momentum information: a resolution of the order of nanoseconds can be used to separate sub-GeV muons from muons with higher energies. Resistive plate chambers have a low cost per unit area and good spatial and time resolution; these features make them an excellent choice as detectors for muon tomography. In order to instrument a large area demonstrator we have produced 25 new readout boards and 30 glass RPCs. The RPCs measure 1800 mm× 600 mm and are read out using 1.68 mm pitch copper strips. The chambers were tested with a standardized procedure, i.e. without optimizing the working parameters to take into account differences in the manufacturing process, and the results show that the RPCs have an efficiency between 87% and 95%. The readout electronics show a signal to noise ratio greater than 20 for minimum ionizing particles. Spatial resolution better than 500 μm can easily be achieved using commercial read out ASICs. These results are better than the original minimum requirements to pass the tests and we are now ready to install the detectors.

The Q Exactive HF, a Benchtop Mass Spectrometer with a Pre-filter, High-performance Quadrupole and an Ultra-high-field Orbitrap Analyzer*

PubMed Central

Scheltema, Richard Alexander; Hauschild, Jan-Peter; Lange, Oliver; Hornburg, Daniel; Denisov, Eduard; Damoc, Eugen; Kuehn, Andreas; Makarov, Alexander; Mann, Matthias

2014-01-01

The quadrupole Orbitrap mass spectrometer (Q Exactive) made a powerful proteomics instrument available in a benchtop format. It significantly boosted the number of proteins analyzable per hour and has now evolved into a proteomics analysis workhorse for many laboratories. Here we describe the Q Exactive Plus and Q Exactive HF mass spectrometers, which feature several innovations in comparison to the original Q Exactive instrument. A low-resolution pre-filter has been implemented within the injection flatapole, preventing unwanted ions from entering deep into the system, and thereby increasing its robustness. A new segmented quadrupole, with higher fidelity of isolation efficiency over a wide range of isolation windows, provides an almost 2-fold improvement of transmission at narrow isolation widths. Additionally, the Q Exactive HF has a compact Orbitrap analyzer, leading to higher field strength and almost doubling the resolution at the same transient times. With its very fast isolation and fragmentation capabilities, the instrument achieves overall cycle times of 1 s for a top 15 to 20 higher energy collisional dissociation method. We demonstrate the identification of 5000 proteins in standard 90-min gradients of tryptic digests of mammalian cell lysate, an increase of over 40% for detected peptides and over 20% for detected proteins. Additionally, we tested the instrument on peptide phosphorylation enriched samples, for which an improvement of up to 60% class I sites was observed. PMID:25360005

Neural Imaging Using Single-Photon Avalanche Diodes

PubMed Central

Karami, Mohammad Azim; Ansarian, Misagh

2017-01-01

Introduction: This paper analyses the ability of single-photon avalanche diodes (SPADs) for neural imaging. The current trend in the production of SPADs moves toward the minimum dark count rate (DCR) and maximum photon detection probability (PDP). Moreover, the jitter response which is the main measurement characteristic for the timing uncertainty is progressing. Methods: The neural imaging process using SPADs can be performed by means of florescence lifetime imaging (FLIM), time correlated single-photon counting (TCSPC), positron emission tomography (PET), and single-photon emission computed tomography (SPECT). Results: This trend will result in more precise neural imaging cameras. While achieving low DCR SPADs is difficult in deep submicron technologies because of using higher doping profiles, higher PDPs are reported in green and blue part of light. Furthermore, the number of pixels integrated in the same chip is increasing with the technology progress which can result in the higher resolution of imaging. Conclusion: This study proposes implemented SPADs in Deep-submicron technologies to be used in neural imaging cameras, due to the small size pixels and higher timing accuracies. PMID:28446946

Recent developments in heterodyne laser interferometry at Harbin Institute of Technology

NASA Astrophysics Data System (ADS)

Hu, P. C.; Tan, J. B. B.; Yang, H. X. X.; Fu, H. J. J.; Wang, Q.

2013-01-01

In order to fulfill the requirements for high-resolution and high-precision heterodyne interferometric technologies and instruments, the laser interferometry group of HIT has developed some novel techniques for high-resolution and high-precision heterodyne interferometers, such as high accuracy laser frequency stabilization, dynamic sub-nanometer resolution phase interpolation and dynamic nonlinearity measurement. Based on a novel lock point correction method and an asymmetric thermal structure, the frequency stabilized laser achieves a long term stability of 1.2×10-8, and it can be steadily stabilized even in the air flowing up to 1 m/s. In order to achieve dynamic sub-nanometer resolution of laser heterodyne interferometers, a novel phase interpolation method based on digital delay line is proposed. Experimental results show that, the proposed 0.62 nm, phase interpolator built with a 64 multiple PLL and an 8-tap digital delay line achieves a static accuracy better than 0.31nm and a dynamic accuracy better than 0.62 nm over the velocity ranging from -2 m/s to 2 m/s. Meanwhile, an accuracy beam polarization measuring setup is proposed to check and ensure the light's polarization state of the dual frequency laser head, and a dynamic optical nonlinearity measuring setup is built to measure the optical nonlinearity of the heterodyne system accurately and quickly. Analysis and experimental results show that, the beam polarization measuring setup can achieve an accuracy of 0.03° in ellipticity angles and an accuracy of 0.04° in the non-orthogonality angle respectively, and the optical nonlinearity measuring setup can achieve an accuracy of 0.13°.

Concept for Geostationary Experimental Temperature and Moisture Sounder (GETMS)

NASA Technical Reports Server (NTRS)

Kumer, J. B.; Sterrit, L. W.; Steakley, B. C.; Springer, L. A.; Roche, A. E.; Rosenberg, W. J.; James, T. C.; Shenk, W. E.; Susskind, J.; Chesters, D.

1988-01-01

The concept of the Geostationary Experimental Temperature and Moisture Sounder (GETMS) is described, with special attention given to the system constraints and its performance characteristics. The GETMS concept supports operation in a high-resolution 'nominal experimental mode' that could achieve spectral resolution of the order 0.2/cm in the 4.2-micron region with signal/noise sufficient to achieve temperature profile retrievals with vertical resolution of the order 1 to 2 km and accuracy to 1 K or less. The concept includes a cryogenic module to provide cryogenic cooling of the focal plane. The GETMS functional diagram and diagrams of the GETMS spectrometer and of the cryogenics module are included.

Evaluation of Mass Filtered, Time Dilated, Time-of-Flight Mass Spectrometry

DTIC Science & Technology

2010-01-01

Figure 4.4: Mass resolution dependence on field for selected actinides and surrogates...45 Figure 4.7: Mass resolution dependence on field for selected actinides and actinide surrogates, modeled with no initial...system. A somewhat better mass resolution would need to be achieved in order to separate hydride molecules in the actinide region. However, the

CZT drift strip detectors for high energy astrophysics

NASA Astrophysics Data System (ADS)

Kuvvetli, I.; Budtz-Jørgensen, C.; Caroli, E.; Auricchio, N.

2010-12-01

Requirements for X- and gamma ray detectors for future High Energy Astrophysics missions include high detection efficiency and good energy resolution as well as fine position sensitivity even in three dimensions. We report on experimental investigations on the CZT drift detector developed DTU Space. It is operated in the planar transverse field (PTF) mode, with the purpose of demonstrating that the good energy resolution of the CZT drift detector can be combined with the high efficiency of the PTF configuration. Furthermore, we demonstrated and characterized the 3D sensing capabilities of this detector configuration. The CZT drift strip detector (10 mm×10 mm×2.5 mm) was characterized in both standard illumination geometry, Photon Parallel Field (PPF) configuration and in PTF configuration. The detection efficiency and energy resolution are compared for both configurations . The PTF configuration provided a higher efficiency in agreement with calculations. The detector energy resolution was found to be the same (3 keV FWHM at 122 keV) in both in PPF and PTF . The depth sensing capabilities offered by drift strip detectors was investigated by illuminating the detector using a collimated photon beam of 57Co radiation in PTF configuration. The width (300μm FWHM at 122 keV) of the measured depth distributions was almost equal to the finite beam size. However, the data indicate that the best achievable depth resolution for the CZT drift detector is 90μm FWHM at 122 keV and that it is determined by the electronic noise from the setup.

High Spatiotemporal Resolution Dynamic Contrast-Enhanced MR Enterography in Crohn Disease Terminal Ileitis Using Continuous Golden-Angle Radial Sampling, Compressed Sensing, and Parallel Imaging.

PubMed

Ream, Justin M; Doshi, Ankur; Lala, Shailee V; Kim, Sooah; Rusinek, Henry; Chandarana, Hersh

2015-06-01

The purpose of this article was to assess the feasibility of golden-angle radial acquisition with compress sensing reconstruction (Golden-angle RAdial Sparse Parallel [GRASP]) for acquiring high temporal resolution data for pharmacokinetic modeling while maintaining high image quality in patients with Crohn disease terminal ileitis. Fourteen patients with biopsy-proven Crohn terminal ileitis were scanned using both contrast-enhanced GRASP and Cartesian breath-hold (volume-interpolated breath-hold examination [VIBE]) acquisitions. GRASP data were reconstructed with 2.4-second temporal resolution and fitted to the generalized kinetic model using an individualized arterial input function to derive the volume transfer coefficient (K(trans)) and interstitial volume (v(e)). Reconstructions, including data from the entire GRASP acquisition and Cartesian VIBE acquisitions, were rated for image quality, artifact, and detection of typical Crohn ileitis features. Inflamed loops of ileum had significantly higher K(trans) (3.36 ± 2.49 vs 0.86 ± 0.49 min(-1), p < 0.005) and v(e) (0.53 ± 0.15 vs 0.20 ± 0.11, p < 0.005) compared with normal bowel loops. There were no significant differences between GRASP and Cartesian VIBE for overall image quality (p = 0.180) or detection of Crohn ileitis features, although streak artifact was worse with the GRASP acquisition (p = 0.001). High temporal resolution data for pharmacokinetic modeling and high spatial resolution data for morphologic image analysis can be achieved in the same acquisition using GRASP.

From SPOT 5 to Pleiades HR: evolution of the instrumental specifications

NASA Astrophysics Data System (ADS)

Rosak, A.; Latry, C.; Pascal, V.; Laubier, D.

2017-11-01

Image quality specifications should aimed to fulfil high resolution mission requirements of remote sensing satellites with a minimum cost. The most important trade-off to be taken into account is between Modulation Transfer Function, radiometric noise and sampling scheme. This compromise is the main driver during design optimisation and requirement definition in order to achieve good performances and to minimise the mission cost. For the SPOT 5 satellite, a new compromise had been chosen. The supermode principle of imagery (sampling at 2.5 meter with a pixel size of 5 meter) imp roves the resolution by a factor of four compared with the SPOT 4 satellite (10 meter resolution). This paper presents the image quality specifications of the HRG-SPOT 5 instrument. We introduce all the efforts made on the instrument to achieve good image quality and low radiometric noise, then we compare the results with the SPOT 4 instrument's performances to highlight the improvements achieved. Then, the in-orbit performance will be described. Finally, we will present the new goals of image quality specifications for the new Pleiades-HR satellite for earth observation (0.7 meter resolution) and the instrument concept.

Superconducting thin-film gyroscope readout for Gravity Probe-B

NASA Technical Reports Server (NTRS)

Lockhart, James M.; Cheung, W. Stephen; Gill, Dale K.

1987-01-01

The high-resolution gyroscope readout system for the Stanford Gravity Probe-B experiment, whose purpose is to measure two general relativistic precessions of gyroscopes in earth orbit, is described. In order to achieve the required resolution in angle (0.001 arcsec), the readout system combines high-precision mechanical fabrication and measurement techniques with superconducting thin-film technology, ultralow magnetic fields, and SQUID detectors. The system design, performance limits achievable with current technology, and the results of fabrication and laboratory testing to date are discussed.

Multichannel analyzers at high rates of input

NASA Technical Reports Server (NTRS)

Rudnick, S. J.; Strauss, M. G.

1969-01-01

Multichannel analyzer, used with a gating system incorporating pole-zero compensation, pile-up rejection, and baseline-restoration, achieves good resolution at high rates of input. It improves resolution, reduces tailing and rate-contributed continuum, and eliminates spectral shift.

Adaptive optics stochastic optical reconstruction microscopy (AO-STORM) by particle swarm optimization

PubMed Central

Tehrani, Kayvan F.; Zhang, Yiwen; Shen, Ping; Kner, Peter

2017-01-01

Stochastic optical reconstruction microscopy (STORM) can achieve resolutions of better than 20nm imaging single fluorescently labeled cells. However, when optical aberrations induced by larger biological samples degrade the point spread function (PSF), the localization accuracy and number of localizations are both reduced, destroying the resolution of STORM. Adaptive optics (AO) can be used to correct the wavefront, restoring the high resolution of STORM. A challenge for AO-STORM microscopy is the development of robust optimization algorithms which can efficiently correct the wavefront from stochastic raw STORM images. Here we present the implementation of a particle swarm optimization (PSO) approach with a Fourier metric for real-time correction of wavefront aberrations during STORM acquisition. We apply our approach to imaging boutons 100 μm deep inside the central nervous system (CNS) of Drosophila melanogaster larvae achieving a resolution of 146 nm. PMID:29188105

High Speed Computational Ghost Imaging via Spatial Sweeping

NASA Astrophysics Data System (ADS)

Wang, Yuwang; Liu, Yang; Suo, Jinli; Situ, Guohai; Qiao, Chang; Dai, Qionghai

2017-03-01

Computational ghost imaging (CGI) achieves single-pixel imaging by using a Spatial Light Modulator (SLM) to generate structured illuminations for spatially resolved information encoding. The imaging speed of CGI is limited by the modulation frequency of available SLMs, and sets back its practical applications. This paper proposes to bypass this limitation by trading off SLM’s redundant spatial resolution for multiplication of the modulation frequency. Specifically, a pair of galvanic mirrors sweeping across the high resolution SLM multiply the modulation frequency within the spatial resolution gap between SLM and the final reconstruction. A proof-of-principle setup with two middle end galvanic mirrors achieves ghost imaging as fast as 42 Hz at 80 × 80-pixel resolution, 5 times faster than state-of-the-arts, and holds potential for one magnitude further multiplication by hardware upgrading. Our approach brings a significant improvement in the imaging speed of ghost imaging and pushes ghost imaging towards practical applications.

Achieving high-efficiency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles.

PubMed

Zhan, Qiuqiang; Liu, Haichun; Wang, Baoju; Wu, Qiusheng; Pu, Rui; Zhou, Chao; Huang, Bingru; Peng, Xingyun; Ågren, Hans; He, Sailing

2017-10-20

Stimulated emission depletion microscopy provides a powerful sub-diffraction imaging modality for life science studies. Conventionally, stimulated emission depletion requires a relatively high light intensity to obtain an adequate depletion efficiency through only light-matter interaction. Here we show efficient emission depletion for a class of lanthanide-doped upconversion nanoparticles with the assistance of interionic cross relaxation, which significantly lowers the laser intensity requirements of optical depletion. We demonstrate two-color super-resolution imaging using upconversion nanoparticles (resolution ~ 66 nm) with a single pair of excitation/depletion beams. In addition, we show super-resolution imaging of immunostained cytoskeleton structures of fixed cells (resolution ~ 82 nm) using upconversion nanoparticles. These achievements provide a new perspective for the development of photoswitchable luminescent probes and will broaden the applications of lanthanide-doped nanoparticles for sub-diffraction microscopic imaging.

The Stability of Chandra Telescope Pointing and Spacial Resolution

NASA Astrophysics Data System (ADS)

Zhao, Ping

2018-01-01

Chandra X-ray Observatory revolutionized the X-ray astronomy as being the first, and so far the only, X-ray telescope achieving sub-arcsecond spacial resolution. Chandra is comprised of three principal elements: the High Resolution Mirror Assembly (HRMA), Pointing Control and Aspect Determination (PCAD) system, and the Science Instrument Module (SIM), which is where the X-ray detectors mounted and is connected to the HRMA by a 10-meter long Optical Bench Assembly. To achieve and retain the unprecedented imaging quality, it is critical that these three principal elements to stay rigid and stable for the entire life time of the Chandra operation. I will review the issues of telescope pointing stability, optical Axis, aimpoint and their impacts to the Chandra operation, and evaluate the integrity and stability of the telescope. I will show images taken from all four detectors since launch to demonstrate the quality and stability of the Chandra spacial resolution.

The Quality and Stability of Chandra Telescope Spacial Resolution

NASA Astrophysics Data System (ADS)

Zhao, Ping

2017-08-01

Chandra X-ray Observatory revolutionized the X-ray astronomy as being the first, and so far the only, X-ray telescope achieving sub-arcsecond spacial resolution. Chandra is comprised of three principal elements: the High Resolution Mirror Assembly (HRMA), Pointing Control and Aspect Determination (PCAD) system, and the Science Instrument Module (SIM), which is where the X-ray detectors mounted and is connected to the HRMA by a 10-meter long Optical Bench Assembly. To achieve and retain the unprecedented imaging quality, it is critical that these three principal elements to stay rigid and stable for the entire life time of the Chandra operation. I will review the issues of telescope pointing stability, optical Axis, aimpoint and their impacts to the Chandra operation, and evaluate the integrity and stability of the telescope. I will show images taken from all four detectors since launch to demonstrate the quality and stability of the Chandra spacial resolution.

Theoretical analysis of microring resonator-based biosensor with high resolution and free of temperature influence

NASA Astrophysics Data System (ADS)

Jian, Aoqun; Zou, Lu; Tang, Haiquan; Duan, Qianqian; Ji, Jianlong; Zhang, Qianwu; Zhang, Xuming; Sang, Shengbo

2017-06-01

The issue of thermal effects is inevitable for the ultrahigh refractive index (RI) measurement. A biosensor with parallel-coupled dual-microring resonator configuration is proposed to achieve high resolution and free thermal effects measurement. Based on the coupled-resonator-induced transparency effect, the design and principle of the biosensor are introduced in detail, and the performance of the sensor is deduced by simulations. Compared to the biosensor based on a single-ring configuration, the designed biosensor has a 10-fold increased Q value according to the simulation results, thus the sensor is expected to achieve a particularly high resolution. In addition, the output signal of the mathematical model of the proposed sensor can eliminate the thermal influence by adopting an algorithm. This work is expected to have great application potentials in the areas of high-resolution RI measurement, such as biomedical discoveries, virus screening, and drinking water safety.

Adaptive optics stochastic optical reconstruction microscopy (AO-STORM) by particle swarm optimization.

PubMed

Tehrani, Kayvan F; Zhang, Yiwen; Shen, Ping; Kner, Peter

2017-11-01

Stochastic optical reconstruction microscopy (STORM) can achieve resolutions of better than 20nm imaging single fluorescently labeled cells. However, when optical aberrations induced by larger biological samples degrade the point spread function (PSF), the localization accuracy and number of localizations are both reduced, destroying the resolution of STORM. Adaptive optics (AO) can be used to correct the wavefront, restoring the high resolution of STORM. A challenge for AO-STORM microscopy is the development of robust optimization algorithms which can efficiently correct the wavefront from stochastic raw STORM images. Here we present the implementation of a particle swarm optimization (PSO) approach with a Fourier metric for real-time correction of wavefront aberrations during STORM acquisition. We apply our approach to imaging boutons 100 μm deep inside the central nervous system (CNS) of Drosophila melanogaster larvae achieving a resolution of 146 nm.

Cationic permethylated 6-monoamino-6-monodeoxy-β-cyclodextrin as chiral selector of dansylated amino acids in capillary electrophoresis.

PubMed

Németh, Krisztina; Domonkos, Celesztina; Sarnyai, Virág; Szemán, Julianna; Jicsinszky, László; Szente, Lajos; Visy, Júlia

2014-10-01

The resolution power of permethylated 6-monoamino-6-monodeoxy-βCD (PMMABCD) - a single isomer, cationic CD derivative - developed previously for chiral analyses in capillary electrophoresis was further studied here. Dansylated amino acids (Dns-AA) were chosen as amphoteric chiral model compounds. Changes in the resolutions of Dns-AAs by varying pH and selector concentrations were investigated and correlated with their structures and chemical properties (isoelectric point and lipophilicity). Maximal resolutions could be achieved at pH 6 or pH 4. The separations improved with increasing concentration of the selector. Baseline or substantially better resolution for 8 pairs of these Dns-AAs could be achieved. Low CD concentration was enough for the separation of the most apolar Dns-AAs. Chiral discrimination ability of PMMABCD was demonstrated by the separation of an artificial mixture of 8 Dns-AA pairs. Copyright © 2014 Elsevier B.V. All rights reserved.

Development of Metallic Magnetic Calorimeters for Nuclear Safeguards Applications

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bates, Cameron Russell

2015-03-11

Many nuclear safeguards applications could benefit from high-resolution gamma-ray spectroscopy achievable with metallic magnetic calorimeters. This dissertation covers the development of a system for these applications based on gamma-ray detectors developed at the University of Heidelberg. It demonstrates new calorimeters of this type, which achieved an energy resolution of 45.5 eV full-width at half-maximum at 59.54 keV, roughly ten times better than current state of the art high purity germanium detectors. This is the best energy resolution achieved with a gamma-ray metallic magnetic calorimeter at this energy to date. In addition to demonstrating a new benchmark in energy resolution, anmore » experimental system for measuring samples with metallic magnetic calorimeters was constructed at Lawrence Livermore National Laboratory. This system achieved an energy resolution of 91.3 eV full-width at half-maximum at 59.54 keV under optimal conditions. Using this system it was possible to characterize the linearity of the response, the count-rate limitations, and the energy resolution as a function of temperature of the new calorimeter. With this characterization it was determined that it would be feasible to measure 242Pu in a mixed isotope plutonium sample. A measurement of a mixed isotope plutonium sample was performed over the course of 12 days with a single two-pixel metallic magnetic calorimeter. The relative concentration of 242Pu in comparison to other plutonium isotopes was determined by direct measurement to less than half a percent accuracy. This is comparable with the accuracy of the best-case scenario using traditional indirect methods. The ability to directly measure the relative concentration of 242Pu in a sample could enable more accurate accounting and detection of indications of undeclared activities in nuclear safeguards, a better constraint on source material in forensic samples containing plutonium, and improvements in verification in a future plutonium disposition treaty.« less

Development of a fast multi-line x-ray CT detector for NDT

NASA Astrophysics Data System (ADS)

Hofmann, T.; Nachtrab, F.; Schlechter, T.; Neubauer, H.; Mühlbauer, J.; Schröpfer, S.; Ernst, J.; Firsching, M.; Schweiger, T.; Oberst, M.; Meyer, A.; Uhlmann, N.

2015-04-01

Typical X-ray detectors for non-destructive testing (NDT) are line detectors or area detectors, like e.g. flat panel detectors. Multi-line detectors are currently only available in medical Computed Tomography (CT) scanners. Compared to flat panel detectors, line and multi-line detectors can achieve much higher frame rates. This allows time-resolved 3D CT scans of an object under investigation. Also, an improved image quality can be achieved due to reduced scattered radiation from object and detector themselves. Another benefit of line and multi-line detectors is that very wide detectors can be assembled easily, while flat panel detectors are usually limited to an imaging field with a size of approx. 40 × 40 cm2 at maximum. The big disadvantage of line detectors is the limited number of object slices that can be scanned simultaneously. This leads to long scan times for large objects. Volume scans with a multi-line detector are much faster, but with almost similar image quality. Due to the promising properties of multi-line detectors their application outside of medical CT would also be very interesting for NDT. However, medical CT multi-line detectors are optimized for the scanning of human bodies. Many non-medical applications require higher spatial resolutions and/or higher X-ray energies. For those non-medical applications we are developing a fast multi-line X-ray detector.In the scope of this work, we present the current state of the development of the novel detector, which includes several outstanding properties like an adjustable curved design for variable focus-detector-distances, conserving nearly uniform perpendicular irradiation over the entire detector width. Basis of the detector is a specifically designed, radiation hard CMOS imaging sensor with a pixel pitch of 200 μ m. Each pixel has an automatic in-pixel gain adjustment, which allows for both: a very high sensitivity and a wide dynamic range. The final detector is planned to have 256 lines of pixels. By using a modular assembly of the detector, the width can be chosen as multiples of 512 pixels. With a frame rate of up to 300 frames/s (full resolution) or 1200 frame/s (analog binning to 400 μ m pixel pitch) time-resolved 3D CT applications become possible. Two versions of the detector are in development, one with a high resolution scintillator and one with a thick, structured and very efficient scintillator (pitch 400 μ m). This way the detector can even work with X-ray energies up to 450 kVp.

Aggression, conflict resolution, popularity, and attitude to school in Russian adolescents.

PubMed

Butovskaya, Marina L; Timentschik, Vera M; Burkova, Valentina N

2007-01-01

The objective of the present study was to examine the effects of aggression and conflict-managing skills on popularity and attitude to school in Russian adolescents. Three types of aggression (physical, verbal, and indirect), constructive conflict resolution, third-party intervention, withdrawal, and victimization were examined using the Peer-Estimated Conflict Behavior (PECOBE) inventory [Bjorkquist and Osterman, 1998]. Also, all respondents rated peer and self-popularity with same-sex classmates and personal attitude to school. The sample consisted of 212 Russian adolescents (101 boys, 111 girls) aged between 11 and 15 years. The findings attest to significant sex differences in aggression and conflict resolution patterns. Boys scored higher on physical and verbal aggression, and girls on indirect aggression. Girls were socially more skillful than boys in the use of peaceful means of conflict resolution (they scored higher on constructive conflict resolution and third-party intervention). The attributional discrepancy index (ADI) scores were negative for all three types of aggression in both sexes. Verbal aggression is apparently more condemned in boys than in girls. ADI scores were positive for constructive conflict resolution and third-party intervention in both genders, being higher in boys. In girls, verbal aggression was positively correlated with popularity. In both sexes, popularity showed a positive correlation with constructive conflict resolution and third-party intervention, and a negative correlation with withdrawal and victimization. Boys who liked school were popular with same-sex peers and scored higher on constructive conflict resolution. Girls who liked school were less aggressive according to peer rating. They also rated higher on conflict resolution and third-party intervention. Physical aggression was related to age. The results are discussed in a cross-cultural perspective. Copyright 2007 Wiley-Liss, Inc.

High-Speed Videography Overview

NASA Astrophysics Data System (ADS)

Miller, C. E.

1989-02-01

The field of high-speed videography (HSV) has continued to mature in recent years, due to the introduction of a mixture of new technology and extensions of existing technology. Recent low frame-rate innovations have the potential to dramatically expand the areas of information gathering and motion analysis at all frame-rates. Progress at the 0 - rate is bringing the battle of film versus video to the field of still photography. The pressure to push intermediate frame rates higher continues, although the maximum achievable frame rate has remained stable for several years. Higher maximum recording rates appear technologically practical, but economic factors impose severe limitations to development. The application of diverse photographic techniques to video-based systems is under-exploited. The basics of HSV apply to other fields, such as machine vision and robotics. Present motion analysis systems continue to function mainly as an instant replay replacement for high-speed movie film cameras. The interrelationship among lighting, shuttering and spatial resolution is examined.

Acceleration of high resolution temperature based optimization for hyperthermia treatment planning using element grouping.

PubMed

Kok, H P; de Greef, M; Bel, A; Crezee, J

2009-08-01

In regional hyperthermia, optimization is useful to obtain adequate applicator settings. A speed-up of the previously published method for high resolution temperature based optimization is proposed. Element grouping as described in literature uses selected voxel sets instead of single voxels to reduce computation time. Elements which achieve their maximum heating potential for approximately the same phase/amplitude setting are grouped. To form groups, eigenvalues and eigenvectors of precomputed temperature matrices are used. At high resolution temperature matrices are unknown and temperatures are estimated using low resolution (1 cm) computations and the high resolution (2 mm) temperature distribution computed for low resolution optimized settings using zooming. This technique can be applied to estimate an upper bound for high resolution eigenvalues. The heating potential of elements was estimated using these upper bounds. Correlations between elements were estimated with low resolution eigenvalues and eigenvectors, since high resolution eigenvectors remain unknown. Four different grouping criteria were applied. Constraints were set to the average group temperatures. Element grouping was applied for five patients and optimal settings for the AMC-8 system were determined. Without element grouping the average computation times for five and ten runs were 7.1 and 14.4 h, respectively. Strict grouping criteria were necessary to prevent an unacceptable exceeding of the normal tissue constraints (up to approximately 2 degrees C), caused by constraining average instead of maximum temperatures. When strict criteria were applied, speed-up factors of 1.8-2.1 and 2.6-3.5 were achieved for five and ten runs, respectively, depending on the grouping criteria. When many runs are performed, the speed-up factor will converge to 4.3-8.5, which is the average reduction factor of the constraints and depends on the grouping criteria. Tumor temperatures were comparable. Maximum exceeding of the constraint in a hot spot was 0.24-0.34 degree C; average maximum exceeding over all five patients was 0.09-0.21 degree C, which is acceptable. High resolution temperature based optimization using element grouping can achieve a speed-up factor of 4-8, without large deviations from the conventional method.

Geostationary Operational Environmental Satellites (GOES): R series hyperspectral environmental suite (HES) overview

NASA Astrophysics Data System (ADS)

Martin, Gene; Criscione, Joseph C.; Cauffman, Sandra A.; Davis, Martin A.

2004-11-01

The Hyperspectral Environmental Suite (HES) instrument is currently under development by the NASA GOES-R Project team within the framework of the GOES Program to fulfill the future needs and requirements of the National Environmental Satellite, Data, and Information Service (NESDIS) Office. As part of the GOES-R instrument complement, HES will provide measurements of the traditional temperature and water vapor vertical profiles with higher accuracy and vertical resolution than obtained through current sounder technologies. HES will provide measurements of the properties of the shelf and coastal waters and back up imaging (at in-situ resolution) for the GOES-R Advanced Baseline Imager (ABI). The HES team is forging the future of remote environmental monitoring with the development of an operational instrument with high temporal, spatial and spectral-resolution and broad hemispheric coverage. The HES development vision includes threshold and goal requirements that encompass potential system solutions. The HES team has defined tasks for the instrument(s) that include a threshold functional complement of Disk Sounding (DS), Severe Weather/Mesoscale Sounding (SW/M), and Shelf and Coastal Waters imaging (CW) and a goal functional complement of Open Ocean (OO) imaging, and back up imaging (at in-situ resolution) for the GOES-R Advanced Baseline Imager (ABI). To achieve the best-value procurement, the GOES-R Project has base-lined a two-phase procurement approach to the HES design and development; a Formulation/study phase and an instrument Implementation phase. During Formulation, currently slated for the FY04-05 timeframe, the developing team(s) will perform Systems Requirements Analysis and evaluation, System Trade and Requirements Baseline Studies, Risk Assessment and Mitigation Strategy and complete a Preliminary Conceptual Design of the HES instrument. The results of the formulation phase will be leveraged to achieve an effective and efficient system solution during the Implementation Phase scheduled to begin FY05 for a resultant FY12 launch. The magnitude of complexity of the HES development requires an appreciation of the technologies required to achieve the functional objectives. To this end, the GOES-R project team is making available all NASA developed technologies to potential HES vendors, including, the NASA New Millennium Program"s (NMP) Earth Observing-3, Geostationary Imaging Fourier Transform Spectrometer (GIFTS) instrument developed technologies, as applicable. It is anticipated that the instrument(s) meeting the HES requirements will be either a dispersive spectrometer or an interferometric spectrometer or perhaps a combination. No instrument configuration is preferred or favored by the Government. This paper outlines the HES development plan; including an overview of current requirements, existing partnerships and the GOES-R project methodologies to achieve the advanced functional objectives of the GOES Program partnership.

Musculoskeletal MRI at 3.0 T and 7.0 T: a comparison of relaxation times and image contrast.

PubMed

Jordan, Caroline D; Saranathan, Manojkumar; Bangerter, Neal K; Hargreaves, Brian A; Gold, Garry E

2013-05-01

The purpose of this study was to measure and compare the relaxation times of musculoskeletal tissues at 3.0 T and 7.0 T, and to use these measurements to select appropriate parameters for musculoskeletal protocols at 7.0 T. We measured the T₁ and T₂ relaxation times of cartilage, muscle, synovial fluid, bone marrow and subcutaneous fat at both 3.0 T and 7.0 T in the knees of five healthy volunteers. The T₁ relaxation times were measured using a spin-echo inversion recovery sequence with six inversion times. The T₂ relaxation times were measured using a spin-echo sequence with seven echo times. The accuracy of both the T₁ and T₂ measurement techniques was verified in phantoms at both magnetic field strengths. We used the measured relaxation times to help design 7.0 T musculoskeletal protocols that preserve the favorable contrast characteristics of our 3.0 T protocols, while achieving significantly higher resolution at higher SNR efficiency. The T₁ relaxation times in all tissues at 7.0 T were consistently higher than those measured at 3.0 T, while the T₂ relaxation times at 7.0 T were consistently lower than those measured at 3.0 T. The measured relaxation times were used to help develop high resolution 7.0 T protocols that had similar fluid-to-cartilage contrast to that of the standard clinical 3.0 T protocols for the following sequences: proton-density-weighted fast spin-echo (FSE), T₂-weighted FSE, and 3D-FSE-Cube. The T₁ and T₂ changes were within the expected ranges. Parameters for musculoskeletal protocols at 7.0 T can be optimized based on these values, yielding improved resolution in musculoskeletal imaging with similar contrast to that of standard 3.0 T clinical protocols. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

FSR: feature set reduction for scalable and accurate multi-class cancer subtype classification based on copy number.

PubMed

Wong, Gerard; Leckie, Christopher; Kowalczyk, Adam

2012-01-15

Feature selection is a key concept in machine learning for microarray datasets, where features represented by probesets are typically several orders of magnitude larger than the available sample size. Computational tractability is a key challenge for feature selection algorithms in handling very high-dimensional datasets beyond a hundred thousand features, such as in datasets produced on single nucleotide polymorphism microarrays. In this article, we present a novel feature set reduction approach that enables scalable feature selection on datasets with hundreds of thousands of features and beyond. Our approach enables more efficient handling of higher resolution datasets to achieve better disease subtype classification of samples for potentially more accurate diagnosis and prognosis, which allows clinicians to make more informed decisions in regards to patient treatment options. We applied our feature set reduction approach to several publicly available cancer single nucleotide polymorphism (SNP) array datasets and evaluated its performance in terms of its multiclass predictive classification accuracy over different cancer subtypes, its speedup in execution as well as its scalability with respect to sample size and array resolution. Feature Set Reduction (FSR) was able to reduce the dimensions of an SNP array dataset by more than two orders of magnitude while achieving at least equal, and in most cases superior predictive classification performance over that achieved on features selected by existing feature selection methods alone. An examination of the biological relevance of frequently selected features from FSR-reduced feature sets revealed strong enrichment in association with cancer. FSR was implemented in MATLAB R2010b and is available at http://ww2.cs.mu.oz.au/~gwong/FSR.

A randomized, placebo‐controlled trial of cenicriviroc for treatment of nonalcoholic steatohepatitis with fibrosis

PubMed Central

Ratziu, Vlad; Harrison, Stephen A.; Abdelmalek, Manal F.; Aithal, Guruprasad P.; Caballeria, Juan; Francque, Sven; Farrell, Geoffrey; Kowdley, Kris V.; Craxi, Antonio; Simon, Krzysztof; Fischer, Laurent; Melchor‐Khan, Liza; Vest, Jeffrey; Wiens, Brian L.; Vig, Pamela; Seyedkazemi, Star; Goodman, Zachary; Wong, Vincent Wai‐Sun; Loomba, Rohit; Tacke, Frank; Sanyal, Arun; Lefebvre, Eric

2018-01-01

The aim of this study was to evaluate cenicriviroc (CVC), a dual antagonist of C—C chemokine receptor types 2 and 5, for treatment of nonalcoholic steatohepatitis (NASH) with liver fibrosis (LF). A randomized, double‐blind, multinational phase 2b study enrolled subjects with NASH, a nonalcoholic fatty liver disease activity score (NAS) ≥4, and LF (stages 1‐3, NASH Clinical Research Network) at 81 clinical sites. Subjects (N = 289) were randomly assigned CVC 150 mg or placebo. Primary outcome was ≥2‐point improvement in NAS and no worsening of fibrosis at year 1. Key secondary outcomes were: resolution of steatohepatitis (SH) and no worsening of fibrosis; improvement in fibrosis by ≥1 stage and no worsening of SH. Biomarkers of inflammation and adverse events were assessed. Full study recruitment was achieved. The primary endpoint of NAS improvement in the intent‐to‐treat population and resolution of SH was achieved in a similar proportion of subjects on CVC (N = 145) and placebo (N = 144; 16% vs. 19%, P = 0.52 and 8% vs. 6%, P = 0.49, respectively). However, the fibrosis endpoint was met in significantly more subjects on CVC than placebo (20% vs. 10%; P = 0.02). Treatment benefits were greater in those with higher disease activity and fibrosis stage at baseline. Biomarkers of systemic inflammation were reduced with CVC. Safety and tolerability of CVC were comparable to placebo. Conclusion: After 1 year of CVC treatment, twice as many subjects achieved improvement in fibrosis and no worsening of SH compared with placebo. Given the urgent need to develop antifibrotic therapies in NASH, these findings warrant phase 3 evaluation. (Hepatology 2018;67:1754‐1767). PMID:28833331

Multimode simulations of a wide field of view double-Fourier far-infrared spatio-spectral interferometer

NASA Astrophysics Data System (ADS)

Bracken, Colm P.; Lightfoot, John; O'Sullivan, Creidhe; Murphy, J. Anthony; Donohoe, Anthony; Savini, Giorgio; Juanola-Parramon, Roser; The Fisica Consortium, On Behalf Of

2018-01-01

In the absence of 50-m class space-based observatories, subarcsecond astronomy spanning the full far-infrared wavelength range will require space-based long-baseline interferometry. The long baselines of up to tens of meters are necessary to achieve subarcsecond resolution demanded by science goals. Also, practical observing times command a field of view toward an arcminute (1‧) or so, not achievable with a single on-axis coherent detector. This paper is concerned with an application of an end-to-end instrument simulator PyFIInS, developed as part of the FISICA project under funding from the European Commission's seventh Framework Programme for Research and Technological Development (FP7). Predicted results of wide field of view spatio-spectral interferometry through simulations of a long-baseline, double-Fourier, far-infrared interferometer concept are presented and analyzed. It is shown how such an interferometer, illuminated by a multimode detector can recover a large field of view at subarcsecond angular resolution, resulting in similar image quality as that achieved by illuminating the system with an array of coherent detectors. Through careful analysis, the importance of accounting for the correct number of higher-order optical modes is demonstrated, as well as accounting for both orthogonal polarizations. Given that it is very difficult to manufacture waveguide and feed structures at sub-mm wavelengths, the larger multimode design is recommended over the array of smaller single mode detectors. A brief note is provided in the conclusion of this paper addressing a more elegant solution to modeling far-infrared interferometers, which holds promise for improving the computational efficiency of the simulations presented here.

Using ultrahigh sensitive optical microangiography to achieve comprehensive depth resolved microvasculature mapping for human retina

NASA Astrophysics Data System (ADS)

An, Lin; Shen, Tueng T.; Wang, Ruikang K.

2011-10-01

This paper presents comprehensive and depth-resolved retinal microvasculature images within human retina achieved by a newly developed ultrahigh sensitive optical microangiography (UHS-OMAG) system. Due to its high flow sensitivity, UHS-OMAG is much more sensitive to tissue motion due to the involuntary movement of the human eye and head compared to the traditional OMAG system. To mitigate these motion artifacts on final imaging results, we propose a new phase compensation algorithm in which the traditional phase-compensation algorithm is repeatedly used to efficiently minimize the motion artifacts. Comparatively, this new algorithm demonstrates at least 8 to 25 times higher motion tolerability, critical for the UHS-OMAG system to achieve retinal microvasculature images with high quality. Furthermore, the new UHS-OMAG system employs a high speed line scan CMOS camera (240 kHz A-line scan rate) to capture 500 A-lines for one B-frame at a 400 Hz frame rate. With this system, we performed a series of in vivo experiments to visualize the retinal microvasculature in humans. Two featured imaging protocols are utilized. The first is of the low lateral resolution (16 μm) and a wide field of view (4 × 3 mm2 with single scan and 7 × 8 mm2 for multiple scans), while the second is of the high lateral resolution (5 μm) and a narrow field of view (1.5 × 1.2 mm2 with single scan). The great imaging performance delivered by our system suggests that UHS-OMAG can be a promising noninvasive alternative to the current clinical retinal microvasculature imaging techniques for the diagnosis of eye diseases with significant vascular involvement, such as diabetic retinopathy and age-related macular degeneration.

Optimized light sharing for high-resolution TOF PET detector based on digital silicon photomultipliers.

PubMed

Marcinkowski, R; España, S; Van Holen, R; Vandenberghe, S

2014-12-07

The majority of current whole-body PET scanners are based on pixelated scintillator arrays with a transverse pixel size of 4 mm. However, recent studies have shown that decreasing the pixel size to 2 mm can significantly improve image spatial resolution. In this study, the performance of Digital Photon Counter (DPC) from Philips Digital Photon Counting (PDPC) was evaluated to determine their potential for high-resolution whole-body time of flight (TOF) PET scanners. Two detector configurations were evaluated. First, the DPC3200-44-22 DPC array was coupled to a LYSO block of 15 × 15 2 × 2 × 22 mm(3) pixels through a 1 mm thick light guide. Due to light sharing among the dies neighbour logic of the DPC was used. In a second setup the same DPC was coupled directly to a scalable 4 × 4 LYSO matrix of 1.9 × 1.9 × 22 mm(3) crystals with a dedicated reflector arrangement allowing for controlled light sharing patterns inside the matrix. With the first approach an average energy resolution of 14.5% and an average CRT of 376 ps were achieved. For the second configuration an average energy resolution of 11% and an average CRT of 295 ps were achieved. Our studies show that the DPC is a suitable photosensor for a high-resolution TOF-PET detector. The dedicated reflector arrangement allows one to achieve better performances than the light guide approach. The count loss, caused by dark counts, is overcome by fitting the matrix size to the size of DPC single die.

Prospects for Electron Imaging with Ultrafast Time Resolution

DOE Office of Scientific and Technical Information (OSTI.GOV)

Armstrong, M R; Reed, B W; Torralva, B R

2007-01-26

Many pivotal aspects of material science, biomechanics, and chemistry would benefit from nanometer imaging with ultrafast time resolution. Here we demonstrate the feasibility of short-pulse electron imaging with t10 nanometer/10 picosecond spatio-temporal resolution, sufficient to characterize phenomena that propagate at the speed of sound in materials (1-10 kilometer/second) without smearing. We outline resolution-degrading effects that occur at high current density followed by strategies to mitigate these effects. Finally, we present a model electron imaging system that achieves 10 nanometer/10 picosecond spatio-temporal resolution.

Ultrahigh Frequency (100 MHz–300 MHz) Ultrasonic Transducers for Optical Resolution Medical Imagining

PubMed Central

Fei, Chunlong; Chiu, Chi Tat; Chen, Xiaoyang; Chen, Zeyu; Ma, Jianguo; Zhu, Benpeng; Shung, K. Kirk; Zhou, Qifa

2016-01-01

High resolution ultrasonic imaging requires high frequency wide band ultrasonic transducers, which produce short pulses and highly focused beam. However, currently the frequency of ultrasonic transducers is limited to below 100 MHz, mainly because of the challenge in precise control of fabrication parameters. This paper reports the design, fabrication, and characterization of sensitive broadband lithium niobate (LiNbO3) single element ultrasonic transducers in the range of 100–300 MHz, as well as their applications in high resolution imaging. All transducers were built for an f-number close to 1.0, which was achieved by press-focusing the piezoelectric layer into a spherical curvature. Characterization results demonstrated their high sensitivity and a −6 dB bandwidth greater than 40%. Resolutions better than 6.4 μm in the lateral direction and 6.2 μm in the axial direction were achieved by scanning a 4 μm tungsten wire target. Ultrasonic biomicroscopy images of zebrafish eyes were obtained with these transducers which demonstrate the feasibility of high resolution imaging with a performance comparable to optical resolution. PMID:27329379

Quantum interpolation for high-resolution sensing

PubMed Central

Ajoy, Ashok; Liu, Yi-Xiang; Saha, Kasturi; Marseglia, Luca; Jaskula, Jean-Christophe; Bissbort, Ulf; Cappellaro, Paola

2017-01-01

Recent advances in engineering and control of nanoscale quantum sensors have opened new paradigms in precision metrology. Unfortunately, hardware restrictions often limit the sensor performance. In nanoscale magnetic resonance probes, for instance, finite sampling times greatly limit the achievable sensitivity and spectral resolution. Here we introduce a technique for coherent quantum interpolation that can overcome these problems. Using a quantum sensor associated with the nitrogen vacancy center in diamond, we experimentally demonstrate that quantum interpolation can achieve spectroscopy of classical magnetic fields and individual quantum spins with orders of magnitude finer frequency resolution than conventionally possible. Not only is quantum interpolation an enabling technique to extract structural and chemical information from single biomolecules, but it can be directly applied to other quantum systems for superresolution quantum spectroscopy. PMID:28196889

Quantum interpolation for high-resolution sensing.

PubMed

Ajoy, Ashok; Liu, Yi-Xiang; Saha, Kasturi; Marseglia, Luca; Jaskula, Jean-Christophe; Bissbort, Ulf; Cappellaro, Paola

2017-02-28

Recent advances in engineering and control of nanoscale quantum sensors have opened new paradigms in precision metrology. Unfortunately, hardware restrictions often limit the sensor performance. In nanoscale magnetic resonance probes, for instance, finite sampling times greatly limit the achievable sensitivity and spectral resolution. Here we introduce a technique for coherent quantum interpolation that can overcome these problems. Using a quantum sensor associated with the nitrogen vacancy center in diamond, we experimentally demonstrate that quantum interpolation can achieve spectroscopy of classical magnetic fields and individual quantum spins with orders of magnitude finer frequency resolution than conventionally possible. Not only is quantum interpolation an enabling technique to extract structural and chemical information from single biomolecules, but it can be directly applied to other quantum systems for superresolution quantum spectroscopy.

Conflicts and communication between high-achieving Chinese American adolescents and their parents.

PubMed

Qin, Desiree Baolian; Chang, Tzu-Fen; Han, Eun-Jin; Chee, Grace

2012-01-01

Drawing on in-depth interview data collected on 18 high-achieving Chinese American students, the authors examine domains of acculturation-based conflicts, parent and child internal conflicts, and conflict resolution in their families. Their analyses show that well-established negative communication patterns in educational expectations, divergent attitudes toward other races and country of origin, and cultural and language barriers contributed to parent-child conflicts. Their findings also illustrate important internal conflicts both adolescents and parents had along the cultural tightrope of autonomy and relatedness. Finally, the vertical in-group conflict resolution style that was evidenced in youths' accounts raises questions about cultural differences in constructive versus destructive conflict resolution styles. © 2012 Wiley Periodicals, Inc., A Wiley Company.

Non-ECG-gated unenhanced MRA of the carotids: optimization and clinical feasibility.

PubMed

Raoult, H; Gauvrit, J Y; Schmitt, P; Le Couls, V; Bannier, E

2013-11-01

To optimise and assess the clinical feasibility of a carotid non-ECG-gated unenhanced MRA sequence. Sixteen healthy volunteers and 11 patients presenting with internal carotid artery (ICA) disease underwent large field-of-view balanced steady-state free precession (bSSFP) unenhanced MRA at 3T. Sampling schemes acquiring the k-space centre either early (kCE) or late (kCL) in the acquisition window were evaluated. Signal and image quality was scored in comparison to ECG-gated kCE unenhanced MRA and TOF. For patients, computed tomography angiography was used as the reference. In volunteers, kCE sampling yielded higher image quality than kCL and TOF, with fewer flow artefacts and improved signal homogeneity. kCE unenhanced MRA image quality was higher without ECG-gating. Arterial signal and artery/vein contrast were higher with both bSSFP sampling schemes than with TOF. The kCE sequence allowed correct quantification of ten significant stenoses, and it facilitated the identification of an infrapetrous dysplasia, which was outside of the TOF imaging coverage. Non-ECG-gated bSSFP carotid imaging offers high-quality images and is a promising sequence for carotid disease diagnosis in a short acquisition time with high spatial resolution and a large field of view. • Non-ECG-gated unenhanced bSSFP MRA offers high-quality imaging of the carotid arteries. • Sequences using early acquisition of the k-space centre achieve higher image quality. • Non-ECG-gated unenhanced bSSFP MRA allows quantification of significant carotid stenosis. • Short MR acquisition times and ungated sequences are helpful in clinical practice. • High 3D spatial resolution and a large field of view improve diagnostic performance.

[3 Tesla MRI: successful results with higher field strengths].

PubMed

Schmitt, F; Grosu, D; Mohr, C; Purdy, D; Salem, K; Scott, K T; Stoeckel, B

2004-01-01

The recent development of 3 Tesla MRI (3T MRI) has been fueled by promise of increased signal-to-noise ratio(SNR). Many are excited about the opportunity to not only use the increased SNR for clearer images, but also the chance to exchange it for better resolution or faster scans. These possibilities have caused a rapid increase in the market for 3T MRI, where the faster scanning tips an already advantageous economic outlook in favor of the user. As a result, the global market for 3T has grown from a research only market just a few years ago to an ever-increasing clinically oriented customer base. There are, however, significant obstacles to 3T MRI presented by the physics at higher field strengths. For example, the T1 relaxation times are prolonged with increasing magnet field strength. Further, the increased RF-energy deposition (SAR), the larger the chemical shift and the stronger susceptibility effect have to be considered as challenges. It is critical that one looks at both the advantages and disadvantages of using 3T. While there are many issues to address aand a number of different methods for doing so, to properly tackle each of these concerns will take time and effort on the part od researchers and clinicians. The optimization of 3T MRI scanning will have to be a combined effort, though much of the work to date has been in neuroimaging. Multiple applications have been explored in addition to clinical anatomical imaging, where resolution is improved showing structure in the brain never seen before in human MRI. Body and cardiac imaging provide a great challenge but are also achievable at 3T. As an example, the full range of clinical applications currently achieved on today's state-of-the-art 1.5T cardiac MR scanners has also been demonstrated at 3T. In the body, the full range of contrast is available over large fields of view allowing whole liver studies in the clinic or, as needed, one may choose a smaller field of view for high-resolution imaging of the pancreas. The ability to increase resolution for musculoskeletal imaging has provided previously unseen detail. Bone structure, cartilage, and tendons and ligaments can be clearly visualized and pathology more easily detected due to an increased image quality. As the increase in field strength continues, a push to look at 7T has begun. The design philosophy is to keep the system as similar as possible, while changing only the frequency-dependent components. To date, both animal and human imaging have been performed on a whole body 7T scanner. Results show promise for both detailed imaging and functional MRI, but the road ahead is too long to be able to predict where it will end. The move toward higher field strengths is an exciting adventure in which 3T plays the role of trailblazer.

Optimization schemes for the inversion of Bouguer gravity anomalies

NASA Astrophysics Data System (ADS)

Zamora, Azucena

Data sets obtained from measurable physical properties of the Earth structure have helped advance the understanding of its tectonic and structural processes and constitute key elements for resource prospecting. 2-Dimensional (2-D) and 3-D models obtained from the inversion of geophysical data sets are widely used to represent the structural composition of the Earth based on physical properties such as density, seismic wave velocities, magnetic susceptibility, conductivity, and resistivity. The inversion of each one of these data sets provides structural models whose consistency depends on the data collection process, methodology, and overall assumptions made in their individual mathematical processes. Although sampling the same medium, seismic and non-seismic methods often provide inconsistent final structural models of the Earth with varying accuracy, sensitivity, and resolution. Taking two or more geophysical data sets with complementary characteristics (e.g. having higher resolution at different depths) and combining their individual strengths to create a new improved structural model can help achieve higher accuracy and resolution power with respect to its original components while reducing their ambiguity and uncertainty effects. Gravity surveying constitutes a cheap, non-invasive, and non-destructive passive remote sensing method that helps to delineate variations in the gravity field. These variations can originate from regional anomalies due to deep density variations or from residual anomalies related to shallow density variations [41]. Since gravity anomaly inversions suffer from significant non-uniqueness (allowing two or more distinct density structures to have the same gravity signature) and small changes in parameters can highly impact the resulting model, the inversion of gravity data represents an ill-posed mathematical problem. However, gravity studies have demonstrated the effectiveness of this method to trace shallow subsurface density variations associated with structural changes [16]; therefore, it complements those geophysical methods with the same depth resolution that sample a different physical property (e.g. electromagnetic surveys sampling electric conductivity) or even those with different depth resolution sampling an alternative physical property (e.g. large scale seismic reflection surveys imaging the crust and top upper mantle using seismic velocity fields). In order to improve the resolution of Bouguer gravity anomalies, and reduce their ambiguity and uncertainty for the modeling of the shallow crust, we propose the implementation of primal-dual interior point methods for the optimization of density structure models through the introduction of physical constraints for transitional areas obtained from previously acquired geophysical data sets. This dissertation presents in Chapter 2 an initial forward model implementation for the calculation of Bouguer gravity anomalies in the Porphyry Copper-Molybdenum (Cu-Mo) Copper Flat Mine region located in Sierra County, New Mexico. In Chapter 3, we present a constrained optimization framework (using interior-point methods) for the inversion of 2-D models of Earth structures delineating density contrasts of anomalous bodies in uniform regions and/or boundaries between layers in layered environments. We implement the proposed algorithm using three different synthetic gravitational data sets with varying complexity. Specifically, we improve the 2-dimensional density structure models by getting rid of unacceptable solutions (geologically unfeasible models or those not satisfying the required constraints) given the reduction of the solution space. Chapter 4 shows the results from the implementation of our algorithm for the inversion of gravitational data obtained from the area surrounding the Porphyry Cu-Mo Cooper Flat Mine in Sierra County, NM. Information obtained from previous induced polarization surveys and core samples served as physical constraints for the inversion parameters. Finally, in order to achieve higher resolution, Chapter 5 introduces a 3-D theoretical framework for the joint inversion of Bouguer gravity anomalies and surface wave dispersion using interior-point methods. Through this work, we expect to contribute to the creation of additional tools for the development of 2- and 3-D models depicting the Earth's geological processes and to the widespread use of constrained optimization techniques for the inversion of geophysical data sets.

Blind identification of full-field vibration modes of output-only structures from uniformly-sampled, possibly temporally-aliased (sub-Nyquist), video measurements

NASA Astrophysics Data System (ADS)

Yang, Yongchao; Dorn, Charles; Mancini, Tyler; Talken, Zachary; Nagarajaiah, Satish; Kenyon, Garrett; Farrar, Charles; Mascareñas, David

2017-03-01

Enhancing the spatial and temporal resolution of vibration measurements and modal analysis could significantly benefit dynamic modelling, analysis, and health monitoring of structures. For example, spatially high-density mode shapes are critical for accurate vibration-based damage localization. In experimental or operational modal analysis, higher (frequency) modes, which may be outside the frequency range of the measurement, contain local structural features that can improve damage localization as well as the construction and updating of the modal-based dynamic model of the structure. In general, the resolution of vibration measurements can be increased by enhanced hardware. Traditional vibration measurement sensors such as accelerometers have high-frequency sampling capacity; however, they are discrete point-wise sensors only providing sparse, low spatial sensing resolution measurements, while dense deployment to achieve high spatial resolution is expensive and results in the mass-loading effect and modification of structure's surface. Non-contact measurement methods such as scanning laser vibrometers provide high spatial and temporal resolution sensing capacity; however, they make measurements sequentially that requires considerable acquisition time. As an alternative non-contact method, digital video cameras are relatively low-cost, agile, and provide high spatial resolution, simultaneous, measurements. Combined with vision based algorithms (e.g., image correlation or template matching, optical flow, etc.), video camera based measurements have been successfully used for experimental and operational vibration measurement and subsequent modal analysis. However, the sampling frequency of most affordable digital cameras is limited to 30-60 Hz, while high-speed cameras for higher frequency vibration measurements are extremely costly. This work develops a computational algorithm capable of performing vibration measurement at a uniform sampling frequency lower than what is required by the Shannon-Nyquist sampling theorem for output-only modal analysis. In particular, the spatio-temporal uncoupling property of the modal expansion of structural vibration responses enables a direct modal decoupling of the temporally-aliased vibration measurements by existing output-only modal analysis methods, yielding (full-field) mode shapes estimation directly. Then the signal aliasing properties in modal analysis is exploited to estimate the modal frequencies and damping ratios. The proposed method is validated by laboratory experiments where output-only modal identification is conducted on temporally-aliased acceleration responses and particularly the temporally-aliased video measurements of bench-scale structures, including a three-story building structure and a cantilever beam.

Superresolution imaging of Drosophila tissues using expansion microscopy.

PubMed

Jiang, Nan; Kim, Hyeon-Jin; Chozinski, Tyler J; Azpurua, Jorge E; Eaton, Benjamin A; Vaughan, Joshua C; Parrish, Jay Z

2018-06-15

The limited resolving power of conventional diffraction-limited microscopy hinders analysis of small, densely packed structural elements in cells. Expansion microscopy (ExM) provides an elegant solution to this problem, allowing for increased resolution with standard microscopes via physical expansion of the specimen in a swellable polymer hydrogel. Here, we apply, validate, and optimize ExM protocols that enable the study of Drosophila embryos, larval brains, and larval and adult body walls. We achieve a lateral resolution of ∼70 nm in Drosophila tissues using a standard confocal microscope, and we use ExM to analyze fine intracellular structures and intercellular interactions. First, we find that ExM reveals features of presynaptic active zone (AZ) structure that are observable with other superresolution imaging techniques but not with standard confocal microscopy. We further show that synapses known to exhibit age-dependent changes in activity also exhibit age-dependent changes in AZ structure. Finally, we use the significantly improved axial resolution of ExM to show that dendrites of somatosensory neurons are inserted into epithelial cells at a higher frequency than previously reported in confocal microscopy studies. Altogether, our study provides a foundation for the application of ExM to Drosophila tissues and underscores the importance of tissue-specific optimization of ExM procedures.

MOEMS Fabry-Pérot interferometer with point-anchored Si-air mirrors for middle infrared

NASA Astrophysics Data System (ADS)

Tuohiniemi, Mikko; Näsilä, Antti; Akujärvi, Altti; Blomberg, Martti

2014-09-01

We studied how a micromachined Fabry-Pérot interferometer, realized with wide point-anchored Si/air-gap reflectors, performs at the middle-infrared. A computational analysis of the anchor mechanical behavior is also presented. Compared with solid-film reflectors, this technology features better index contrast, which enables a wider stop band and potentially higher resolution. In this work, we investigate whether the performance is improved according to the index-contrast benefit, or whether the mechanical differences play a role. For comparison, we manufactured and characterized another design that applies solid-film reflectors of Si/SiO2 structure. This data is exploited as a reference for a middle-infrared interferometer and as a template for mapping the performance from the simulation results to the measured data. The novel Si/air-gap device was realized as a non-tunable proof-of-concept version. The measured data is mapped into an estimate of the achievable performance of a tunable version. We present the measured transmission and resolution data and compare the simulation models that reproduce the data. The prediction for the tunable middle-infrared Si/air-gap device is then presented. The results indicate that the interferometer’s resolution is expected to have improved twofold and have a much wider stop band compared with the prior art.